LOCUS       DAZFBR010000001       104301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFBR010000001 DAZFBR010000000
VERSION     DAZFBR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 104301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.001"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            86..888
                     /gene="ydjO"
                     /locus_tag="ACHJZ2_000001"
                     /pseudo
     CDS             86..888
                     /gene="ydjO"
                     /locus_tag="ACHJZ2_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(892..2283)
                     /gene="tcyP"
                     /locus_tag="ACHJZ2_000002"
     CDS             complement(892..2283)
                     /gene="tcyP"
                     /locus_tag="ACHJZ2_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO4566207.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(2416..3006)
                     /gene="ydjM"
                     /locus_tag="ACHJZ2_000003"
     CDS             complement(2416..3006)
                     /gene="ydjM"
                     /locus_tag="ACHJZ2_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4566208.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(3169..3837)
                     /gene="hxpB"
                     /locus_tag="ACHJZ2_000004"
     CDS             complement(3169..3837)
                     /gene="hxpB"
                     /locus_tag="ACHJZ2_000004"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO4566209.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3984..4520
                     /gene="yniB"
                     /locus_tag="ACHJZ2_000005"
     CDS             3984..4520
                     /gene="yniB"
                     /locus_tag="ACHJZ2_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO4566210.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4561..5421)
                     /gene="yniA"
                     /locus_tag="ACHJZ2_000006"
     CDS             complement(4561..5421)
                     /gene="yniA"
                     /locus_tag="ACHJZ2_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO4566211.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5527..5817)
                     /gene="ghoS"
                     /locus_tag="ACHJZ2_000007"
     CDS             complement(5527..5817)
                     /gene="ghoS"
                     /locus_tag="ACHJZ2_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO4566212.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5918..6847)
                     /gene="pfkB"
                     /locus_tag="ACHJZ2_000008"
     CDS             complement(5918..6847)
                     /gene="pfkB"
                     /locus_tag="ACHJZ2_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO4566213.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            7134..7892
                     /gene="ydiY"
                     /locus_tag="ACHJZ2_000009"
     CDS             7134..7892
                     /gene="ydiY"
                     /locus_tag="ACHJZ2_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO4566214.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7945..8052)
                     /gene="yniD"
                     /locus_tag="ACHJZ2_000010"
     CDS             complement(7945..8052)
                     /gene="yniD"
                     /locus_tag="ACHJZ2_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO4566215.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(8227..10125)
                     /gene="espL1"
                     /locus_tag="ACHJZ2_000011"
     CDS             complement(8227..10125)
                     /gene="espL1"
                     /locus_tag="ACHJZ2_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFO4566216.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFAPQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENIFITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSIGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTL
                     GAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSMSFS
                     ADELKTYL"
     gene            10648..12576
                     /gene="thrS"
                     /locus_tag="ACHJZ2_000012"
     CDS             10648..12576
                     /gene="thrS"
                     /locus_tag="ACHJZ2_000012"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO4566217.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            12580..13122
                     /gene="infC"
                     /locus_tag="ACHJZ2_000013"
     CDS             12580..13122
                     /gene="infC"
                     /locus_tag="ACHJZ2_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO4566218.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            13219..13416
                     /gene="rpmI"
                     /locus_tag="ACHJZ2_000014"
     CDS             13219..13416
                     /gene="rpmI"
                     /locus_tag="ACHJZ2_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO4566219.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            13469..13825
                     /gene="rplT"
                     /locus_tag="ACHJZ2_000015"
     CDS             13469..13825
                     /gene="rplT"
                     /locus_tag="ACHJZ2_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO4566220.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13942..14066
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13948..13992
                     /gene="pheM"
                     /locus_tag="ACHJZ2_000016"
     CDS             13948..13992
                     /gene="pheM"
                     /locus_tag="ACHJZ2_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO4566221.1"
                     /translation="MNAAIFRFFFYFST"
     gene            14275..15258
                     /gene="pheS"
                     /locus_tag="ACHJZ2_000017"
     CDS             14275..15258
                     /gene="pheS"
                     /locus_tag="ACHJZ2_000017"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO4566222.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            15273..17660
                     /gene="pheT"
                     /locus_tag="ACHJZ2_000018"
     CDS             15273..17660
                     /gene="pheT"
                     /locus_tag="ACHJZ2_000018"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO4566223.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17665..17964
                     /gene="ihfA"
                     /locus_tag="ACHJZ2_000019"
     CDS             17665..17964
                     /gene="ihfA"
                     /locus_tag="ACHJZ2_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO4566224.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            18065..19045
                     /gene="btuC"
                     /locus_tag="ACHJZ2_000020"
     CDS             18065..19045
                     /gene="btuC"
                     /locus_tag="ACHJZ2_000020"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO4566225.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            19108..19659
                     /gene="btuE"
                     /locus_tag="ACHJZ2_000021"
     CDS             19108..19659
                     /gene="btuE"
                     /locus_tag="ACHJZ2_000021"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO4566226.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            19659..20408
                     /gene="btuD"
                     /locus_tag="ACHJZ2_000022"
     CDS             19659..20408
                     /gene="btuD"
                     /locus_tag="ACHJZ2_000022"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO4566227.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            20486..20950
                     /gene="nlpC"
                     /locus_tag="ACHJZ2_000023"
     CDS             20486..20950
                     /gene="nlpC"
                     /locus_tag="ACHJZ2_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO4566228.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            21197..21910
                     /gene="ydiV"
                     /locus_tag="ACHJZ2_000024"
     CDS             21197..21910
                     /gene="ydiV"
                     /locus_tag="ACHJZ2_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO4566229.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            21973..23409
                     /gene="selO"
                     /locus_tag="ACHJZ2_000025"
     CDS             21973..23409
                     /gene="selO"
                     /locus_tag="ACHJZ2_000025"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO4566230.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(23413..23604)
                     /gene="hemP"
                     /locus_tag="ACHJZ2_000026"
     CDS             complement(23413..23604)
                     /gene="hemP"
                     /locus_tag="ACHJZ2_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO4566231.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(23736..24782)
                     /gene="aroH"
                     /locus_tag="ACHJZ2_000027"
     CDS             complement(23736..24782)
                     /gene="aroH"
                     /locus_tag="ACHJZ2_000027"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO4566232.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(24939..25772)
                     /gene="ppsR"
                     /locus_tag="ACHJZ2_000028"
     CDS             complement(24939..25772)
                     /gene="ppsR"
                     /locus_tag="ACHJZ2_000028"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO4566233.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            26105..28483
                     /gene="ppsA"
                     /locus_tag="ACHJZ2_000029"
     CDS             26105..28483
                     /gene="ppsA"
                     /locus_tag="ACHJZ2_000029"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO4566234.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSAKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(28540..30186)
                     /gene="fadK"
                     /locus_tag="ACHJZ2_000030"
     CDS             complement(28540..30186)
                     /gene="fadK"
                     /locus_tag="ACHJZ2_000030"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO4566235.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFTPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCSCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(30243..30536)
                     /gene="ydiT"
                     /locus_tag="ACHJZ2_000031"
     CDS             complement(30243..30536)
                     /gene="ydiT"
                     /locus_tag="ACHJZ2_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO4566236.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(30533..31822)
                     /gene="ydiS"
                     /locus_tag="ACHJZ2_000032"
     CDS             complement(30533..31822)
                     /gene="ydiS"
                     /locus_tag="ACHJZ2_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4566237.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(31878..32816)
                     /gene="ydiR"
                     /locus_tag="ACHJZ2_000033"
     CDS             complement(31878..32816)
                     /gene="ydiR"
                     /locus_tag="ACHJZ2_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO4566238.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(32836..33600)
                     /gene="ydiQ"
                     /locus_tag="ACHJZ2_000034"
     CDS             complement(32836..33600)
                     /gene="ydiQ"
                     /locus_tag="ACHJZ2_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO4566239.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            33916..34827
                     /gene="ydiP"
                     /locus_tag="ACHJZ2_000035"
     CDS             33916..34827
                     /gene="ydiP"
                     /locus_tag="ACHJZ2_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4566240.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(34870..36021)
                     /gene="ydiO"
                     /locus_tag="ACHJZ2_000036"
     CDS             complement(34870..36021)
                     /gene="ydiO"
                     /locus_tag="ACHJZ2_000036"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO4566241.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(36035..37630)
                     /gene="ydiF"
                     /locus_tag="ACHJZ2_000037"
     CDS             complement(36035..37630)
                     /gene="ydiF"
                     /locus_tag="ACHJZ2_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO4566242.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(37775..38533)
                     /gene="aroD"
                     /locus_tag="ACHJZ2_000038"
     CDS             complement(37775..38533)
                     /gene="aroD"
                     /locus_tag="ACHJZ2_000038"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO4566243.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(38564..39430)
                     /gene="ydiB"
                     /locus_tag="ACHJZ2_000039"
     CDS             complement(38564..39430)
                     /gene="ydiB"
                     /locus_tag="ACHJZ2_000039"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO4566244.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(39442..40707)
                     /gene="ydiN"
                     /locus_tag="ACHJZ2_000040"
     CDS             complement(39442..40707)
                     /gene="ydiN"
                     /locus_tag="ACHJZ2_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4566245.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(40934..42148)
                     /gene="ydiM"
                     /locus_tag="ACHJZ2_000041"
     CDS             complement(40934..42148)
                     /gene="ydiM"
                     /locus_tag="ACHJZ2_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4566246.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(42248..42604)
                     /gene="ydiL"
                     /locus_tag="ACHJZ2_000042"
     CDS             complement(42248..42604)
                     /gene="ydiL"
                     /locus_tag="ACHJZ2_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO4566247.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(42740..42847)
                     /gene="rprA"
                     /locus_tag="ACHJZ2_000043"
     ncRNA           complement(42740..42847)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHJZ2_000043"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(43033..44145)
                     /gene="ydiK"
                     /locus_tag="ACHJZ2_000044"
     CDS             complement(43033..44145)
                     /gene="ydiK"
                     /locus_tag="ACHJZ2_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO4566248.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIQKPNK"
     gene            44534..47590
                     /gene="ydiJ"
                     /locus_tag="ACHJZ2_000045"
     CDS             44534..47590
                     /gene="ydiJ"
                     /locus_tag="ACHJZ2_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO4566249.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            47587..47997
                     /gene="menI"
                     /locus_tag="ACHJZ2_000046"
     CDS             47587..47997
                     /gene="menI"
                     /locus_tag="ACHJZ2_000046"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO4566250.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            48097..48285
                     /gene="ydiH"
                     /locus_tag="ACHJZ2_000047"
     CDS             48097..48285
                     /gene="ydiH"
                     /locus_tag="ACHJZ2_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO4566251.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            48834..49202
                     /gene="sufA"
                     /locus_tag="ACHJZ2_000048"
     CDS             48834..49202
                     /gene="sufA"
                     /locus_tag="ACHJZ2_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO4566252.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            49211..50698
                     /gene="sufB"
                     /locus_tag="ACHJZ2_000049"
     CDS             49211..50698
                     /gene="sufB"
                     /locus_tag="ACHJZ2_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO4566253.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            50708..51454
                     /gene="sufC"
                     /locus_tag="ACHJZ2_000050"
     CDS             50708..51454
                     /gene="sufC"
                     /locus_tag="ACHJZ2_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO4566254.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREYYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            51429..52700
                     /gene="sufD"
                     /locus_tag="ACHJZ2_000051"
     CDS             51429..52700
                     /gene="sufD"
                     /locus_tag="ACHJZ2_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO4566255.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            52697..53917
                     /gene="sufS"
                     /locus_tag="ACHJZ2_000052"
     CDS             52697..53917
                     /gene="sufS"
                     /locus_tag="ACHJZ2_000052"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO4566256.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            53930..54346
                     /gene="sufE"
                     /locus_tag="ACHJZ2_000053"
     CDS             53930..54346
                     /gene="sufE"
                     /locus_tag="ACHJZ2_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO4566257.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            54495..55499
                     /gene="ldtE"
                     /locus_tag="ACHJZ2_000054"
     CDS             54495..55499
                     /gene="ldtE"
                     /locus_tag="ACHJZ2_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO4566258.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHDNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(55562..55798)
                     /gene="lpp"
                     /locus_tag="ACHJZ2_000055"
     CDS             complement(55562..55798)
                     /gene="lpp"
                     /locus_tag="ACHJZ2_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO4566259.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(56109..57521)
                     /gene="pykF"
                     /locus_tag="ACHJZ2_000056"
     CDS             complement(56109..57521)
                     /gene="pykF"
                     /locus_tag="ACHJZ2_000056"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO4566260.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(57529..57723)
                     /locus_tag="ACHJZ2_000057"
     CDS             complement(57529..57723)
                     /locus_tag="ACHJZ2_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566261.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            58078..58287
                     /gene="fumD"
                     /locus_tag="ACHJZ2_000058"
     CDS             58078..58287
                     /gene="fumD"
                     /locus_tag="ACHJZ2_000058"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO4566262.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            58743..59369
                     /gene="ydhY"
                     /locus_tag="ACHJZ2_000059"
     CDS             58743..59369
                     /gene="ydhY"
                     /locus_tag="ACHJZ2_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO4566263.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            59390..61486
                     /gene="ydhV"
                     /locus_tag="ACHJZ2_000060"
     CDS             59390..61486
                     /gene="ydhV"
                     /locus_tag="ACHJZ2_000060"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO4566264.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIANRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYSKG
                     VFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWNLG
                     EEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPLKL
                     QREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTVSP
                     LKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRNEH
                     DLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQRLG
                     LEDIAADLAAHNLLPA"
     gene            61499..62137
                     /gene="ydhW"
                     /locus_tag="ACHJZ2_000061"
     CDS             61499..62137
                     /gene="ydhW"
                     /locus_tag="ACHJZ2_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO4566265.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            62201..62869
                     /gene="ydhX"
                     /locus_tag="ACHJZ2_000062"
     CDS             62201..62869
                     /gene="ydhX"
                     /locus_tag="ACHJZ2_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4566266.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            62866..63651
                     /gene="phsC"
                     /locus_tag="ACHJZ2_000063"
     CDS             62866..63651
                     /gene="phsC"
                     /locus_tag="ACHJZ2_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO4566267.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            63655..64467
                     /gene="ydhT"
                     /locus_tag="ACHJZ2_000064"
     CDS             63655..64467
                     /gene="ydhT"
                     /locus_tag="ACHJZ2_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO4566268.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(64479..66083)
                     /gene="ydhS"
                     /locus_tag="ACHJZ2_000065"
     CDS             complement(64479..66083)
                     /gene="ydhS"
                     /locus_tag="ACHJZ2_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO4566269.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(66209..66514)
                     /gene="ydhR"
                     /locus_tag="ACHJZ2_000066"
     CDS             complement(66209..66514)
                     /gene="ydhR"
                     /locus_tag="ACHJZ2_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO4566270.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(66622..66698)
                     /locus_tag="ACHJZ2_000067"
     tRNA            complement(66622..66698)
                     /locus_tag="ACHJZ2_000067"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66662..66664),aa:Val,seq:gac)
     gene            complement(66703..66779)
                     /locus_tag="ACHJZ2_000068"
     tRNA            complement(66703..66779)
                     /locus_tag="ACHJZ2_000068"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66743..66745),aa:Val,seq:gac)
     gene            67087..68343
                     /gene="ydhQ"
                     /locus_tag="ACHJZ2_000069"
     CDS             67087..68343
                     /gene="ydhQ"
                     /locus_tag="ACHJZ2_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO4566271.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(68384..69757)
                     /gene="mdtK"
                     /locus_tag="ACHJZ2_000070"
     CDS             complement(68384..69757)
                     /gene="mdtK"
                     /locus_tag="ACHJZ2_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO4566272.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            69972..70613
                     /gene="ribE"
                     /locus_tag="ACHJZ2_000071"
     CDS             69972..70613
                     /gene="ribE"
                     /locus_tag="ACHJZ2_000071"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO4566273.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(70653..71801)
                     /gene="cfa"
                     /locus_tag="ACHJZ2_000072"
     CDS             complement(70653..71801)
                     /gene="cfa"
                     /locus_tag="ACHJZ2_000072"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO4566274.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(72092..73303)
                     /gene="punC"
                     /locus_tag="ACHJZ2_000073"
     CDS             complement(72092..73303)
                     /gene="punC"
                     /locus_tag="ACHJZ2_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO4566275.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            73416..74348
                     /gene="punR"
                     /locus_tag="ACHJZ2_000074"
     CDS             73416..74348
                     /gene="punR"
                     /locus_tag="ACHJZ2_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO4566276.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(74345..75370)
                     /gene="purR"
                     /locus_tag="ACHJZ2_000075"
     CDS             complement(74345..75370)
                     /gene="purR"
                     /locus_tag="ACHJZ2_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO4566277.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            75669..75758
                     /gene="cydH"
                     /locus_tag="ACHJZ2_000076"
                     /gene_synonym="ynhF"
     CDS             75669..75758
                     /gene="cydH"
                     /locus_tag="ACHJZ2_000076"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO4566278.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            75924..77093
                     /gene="ydhP"
                     /locus_tag="ACHJZ2_000077"
     CDS             75924..77093
                     /gene="ydhP"
                     /locus_tag="ACHJZ2_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4566279.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(77239..77820)
                     /gene="sodB"
                     /locus_tag="ACHJZ2_000078"
     CDS             complement(77239..77820)
                     /gene="sodB"
                     /locus_tag="ACHJZ2_000078"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO4566280.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(77948..78763)
                     /gene="mepH"
                     /locus_tag="ACHJZ2_000079"
     CDS             complement(77948..78763)
                     /gene="mepH"
                     /locus_tag="ACHJZ2_000079"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO4566281.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            79098..79445
                     /gene="grxD"
                     /locus_tag="ACHJZ2_000080"
     CDS             79098..79445
                     /gene="grxD"
                     /locus_tag="ACHJZ2_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO4566282.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(79496..84112)
                     /gene="lhr"
                     /locus_tag="ACHJZ2_000081"
     CDS             complement(79496..84112)
                     /gene="lhr"
                     /locus_tag="ACHJZ2_000081"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO4566283.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVELVIQVATPLSVASGLQRIGRAGHQIGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPAILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVHDYSPEVLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVWQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(84205..84852)
                     /gene="rnt"
                     /locus_tag="ACHJZ2_000082"
     CDS             complement(84205..84852)
                     /gene="rnt"
                     /locus_tag="ACHJZ2_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO4566284.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(84955..85362)
                     /gene="gloA"
                     /locus_tag="ACHJZ2_000083"
     CDS             complement(84955..85362)
                     /gene="gloA"
                     /locus_tag="ACHJZ2_000083"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO4566285.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(85443..86540)
                     /gene="nemA"
                     /locus_tag="ACHJZ2_000084"
     CDS             complement(85443..86540)
                     /gene="nemA"
                     /locus_tag="ACHJZ2_000084"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO4566286.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(86577..87176)
                     /gene="nemR"
                     /locus_tag="ACHJZ2_000085"
     CDS             complement(86577..87176)
                     /gene="nemR"
                     /locus_tag="ACHJZ2_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO4566287.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            87279..87518
                     /gene="ydhL"
                     /locus_tag="ACHJZ2_000086"
     CDS             87279..87518
                     /gene="ydhL"
                     /locus_tag="ACHJZ2_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO4566288.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            87567..88463
                     /gene="ydhF"
                     /locus_tag="ACHJZ2_000087"
                     /pseudo
     CDS             87567..88463
                     /gene="ydhF"
                     /locus_tag="ACHJZ2_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            88544..89065
                     /gene="sodC"
                     /locus_tag="ACHJZ2_000088"
     CDS             88544..89065
                     /gene="sodC"
                     /locus_tag="ACHJZ2_000088"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO4566289.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(89066..91078)
                     /gene="ydhK"
                     /locus_tag="ACHJZ2_000089"
     CDS             complement(89066..91078)
                     /gene="ydhK"
                     /locus_tag="ACHJZ2_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO4566290.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSDVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYNFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGIVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(91078..91935)
                     /gene="ydhJ"
                     /locus_tag="ACHJZ2_000090"
     CDS             complement(91078..91935)
                     /gene="ydhJ"
                     /locus_tag="ACHJZ2_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO4566291.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(91938..92174)
                     /gene="ydhI"
                     /locus_tag="ACHJZ2_000091"
     CDS             complement(91938..92174)
                     /gene="ydhI"
                     /locus_tag="ACHJZ2_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO4566292.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            92375..92809
                     /gene="slyA"
                     /locus_tag="ACHJZ2_000092"
     CDS             92375..92809
                     /gene="slyA"
                     /locus_tag="ACHJZ2_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO4566293.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(92856..93323)
                     /gene="slyB"
                     /locus_tag="ACHJZ2_000093"
     CDS             complement(92856..93323)
                     /gene="slyB"
                     /locus_tag="ACHJZ2_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO4566294.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            93596..94705
                     /gene="anmK"
                     /locus_tag="ACHJZ2_000094"
     CDS             93596..94705
                     /gene="anmK"
                     /locus_tag="ACHJZ2_000094"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO4566295.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            94803..95132
                     /gene="mliC"
                     /locus_tag="ACHJZ2_000095"
     CDS             94803..95132
                     /gene="mliC"
                     /locus_tag="ACHJZ2_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO4566296.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            95191..95847
                     /gene="pdxH"
                     /locus_tag="ACHJZ2_000096"
     CDS             95191..95847
                     /gene="pdxH"
                     /locus_tag="ACHJZ2_000096"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO4566297.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            95976..97250
                     /gene="tyrS"
                     /locus_tag="ACHJZ2_000097"
     CDS             95976..97250
                     /gene="tyrS"
                     /locus_tag="ACHJZ2_000097"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO4566298.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            97312..98172
                     /gene="pdxY"
                     /locus_tag="ACHJZ2_000098"
     CDS             97312..98172
                     /gene="pdxY"
                     /locus_tag="ACHJZ2_000098"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFO4566299.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(98216..98821)
                     /gene="gstA"
                     /locus_tag="ACHJZ2_000099"
     CDS             complement(98216..98821)
                     /gene="gstA"
                     /locus_tag="ACHJZ2_000099"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO4566300.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(98927..100429)
                     /gene="dtpA"
                     /locus_tag="ACHJZ2_000100"
     CDS             complement(98927..100429)
                     /gene="dtpA"
                     /locus_tag="ACHJZ2_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO4566301.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(101040..101675)
                     /gene="nth"
                     /locus_tag="ACHJZ2_000101"
     CDS             complement(101040..101675)
                     /gene="nth"
                     /locus_tag="ACHJZ2_000101"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO4566302.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(101675..102370)
                     /gene="rsxE"
                     /locus_tag="ACHJZ2_000102"
     CDS             complement(101675..102370)
                     /gene="rsxE"
                     /locus_tag="ACHJZ2_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO4566303.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(102374..102994)
                     /gene="rsxG"
                     /locus_tag="ACHJZ2_000103"
     CDS             complement(102374..102994)
                     /gene="rsxG"
                     /locus_tag="ACHJZ2_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO4566304.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            complement(102998..104056)
                     /gene="rsxD"
                     /locus_tag="ACHJZ2_000104"
     CDS             complement(102998..104056)
                     /gene="rsxD"
                     /locus_tag="ACHJZ2_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO4566305.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(104057..>104301)
                     /locus_tag="ACHJZ2_000105"
     CDS             complement(104057..>104301)
                     /locus_tag="ACHJZ2_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO4566306.1"
                     /translation="RAKARKLEQQQANAEPEEQVDPRKAAVAAAIARAKARKLEQQQA
                     NAEPEEQVDPRKAAVAAAIARVQAKKAVQQKVVNED"
BASE COUNT        26537 a        25288 c        26710 g        25766 t
ORIGIN      
        1 tcctgtggcg tcgctcttaa acttatattg taaatgactt agatttaaat gagataataa
       61 ttttgttaac aatatgaggc gggagatgtt ttctacaaac ttaatacaga gtaattatgg
      121 tgatttaaat attaaaagcc tggctttcga ctcttttaaa gaaaggctgc agtctactat
      181 gacagcactc actttcttta tatcaacggg tcaatgtgac tgcgatgaaa tagcagaatc
      241 aaactttaat tatatgattg cctatatgtc taacattaac tatgatgcct caaagccagg
      301 agcgccagca cttagttttg atacatatct gcaggacaat gttaaatata gagtaataat
      361 taataatctt tatggtagtg aaattcggat aagaggtata aataaagatt ttattggaat
      421 ggatgtcact tctgtatttc gcccagaaaa aatgaccaat ttgatcagta taaaaaacag
      481 gttggttatt cactatttaa ggactatata ctatgaacaa tattatatac atcctgtcgg
      541 gagcattttt gctgcgattc aaaaaaatga gagtctccta aagtttcctt cgataatatc
      601 aatgcttaat atcaatcttc tttttaatcc actaaatctt ccgggaatgg gaagtggtat
      661 acttgaagat attatgagta ttccagatag ttcacttaga aaacgtctcg ggtatgaggt
      721 tttaagcttc tcgctacagg cacattcatt gagccaggaa tgtattgata agttggacat
      781 tttttcgctg acgatctgtt taaatatgag tcagtatgca tcgccgcgat ggaacattta
      841 aaaagtaagg caacggcccc tttacaaaac ggaccgttgc cagcataaga attaatggtg
      901 tgccagttcg gcgtcgtctt cgctatccag aatggcttta tcggtttgct tcagccactg
      961 gctggtcagc gtgccagctg tcatcgagcc actaacgttt agcgccgtac ggcccatgtc
     1021 gataagcggt tcaacggaga ttaacagcgc caccagggtt actggcaggc ccatcgcagg
     1081 cagcacaatc agtgcggcga aagttgcacc accaccgaca ccggcaacgc ctgcggagct
     1141 aacggtaaca ataccgacca gcgtcgcaat ccacatcggg tccagcgggt taatgccaac
     1201 cgtaggcgca accatcaccg ccagcattgc cggatacaaa ccggcgcagc cgttctgacc
     1261 aatggtcgca ccgaaagagg cggcgaaact ggcgatggat tcaggaacac cgagtcgacg
     1321 cgtttgtgct tccacattca gtgggataga cgcagcactg gaacggctgg taaaggcaaa
     1381 cgtcagcaca ggccatacct tacggaagta cttcagcgga ctcacgccat taatgcccag
     1441 cagaatgcca tgcactgcaa acataatcag cagaccgagg taggacgcga caacgaaact
     1501 tcccagtttg atgatgtctt gcaggttaga acctgcaacc actttggtca ttagtgccag
     1561 aacgccgtaa ggggtcaact gcatgaccag gcgaactagt ttcatcaccc agctttgcag
     1621 ggtatcgata gcggttaaga cgcgttcacc tttcggcgca tcatccttca gcagtttcag
     1681 cgcagctaca ccgaggaatg cggcaaaaat taccacgctg ataattgacg tcggattggc
     1741 tccggtcaga tcggcaaacg ggtttttcgg gataaaggac aagaccagct gcggaacgct
     1801 caggtcagag actttaccaa catagttact ttcgatggcg ttcagacgtg cagtttctgc
     1861 accaccctga accagacctt cagccgtcaa accaaacagg ttggttacca gcacaccgac
     1921 cagcgccgca atcagcgtgg taaacaaaag cgtaccaatg gtcagaaaac tgattttgcc
     1981 taactgagat gcgttatgca gacgggcaac cgcgctcaga atagaggcga agactaacgg
     2041 cataacgatc atttgcagca gttgaacata gccgttacca acaatgttaa accactgtac
     2101 agaatcttta agtacctggc tgtcagaacc ataaatggta tgcagggcaa ggccaaaaac
     2161 cacacccatc accagaccca ccagcacttt tttcgccaga ctccactgtt tatggcgggt
     2221 ctgagccagc gcaaacagca gtacaacgaa caccacgatg ttcgcaatta atggaaagtt
     2281 catccccgtt ctcctgatct tattatttct atcactgcgt agcagtcata gtgttggcgc
     2341 aagattaaca gaactgtgag ctggctctta tattcaaatg gaattgctta ttacagaaag
     2401 gcgatttttg cctttttagt attcaacctg atgcttaata agccgatgaa actggatctg
     2461 caaggtattg atcatttgcg atgaccaacg aacagcgctg ttttcgggta atgaatgagg
     2521 catccatacc gaccagacaa ataatgccat gcagataaaa cgttccagtt ggttaccctt
     2581 ttggggaacc aggataggca agcggaaacg ccagcggcat ggccagagca ggggaacacc
     2641 ggcgggtgtc agcatatcgg caagtatgtg gcttaaataa cccagcacca ttccttgtag
     2701 cgcatcagcc ggaatgaacc agctttcagg aaccttaagg taaaaggttg ccagcagcgc
     2761 aaataccgcc agcagactgt gggtaaaacc acgatgccca aaagcgcggg cgatcggttt
     2821 tgatatccac tttaatcgct gcccaagaaa cgactttggg tgatcgatgt ccggtaacaa
     2881 acacgtcagg attgcggaag ggacaatatg ccaccagtca ccctgtgcca gcacgggcgt
     2941 cagctcggca tttttggcaa ataccgcaca agcaatagaa aagagaaggt gaccttccgc
     3001 cgtcatgata aaactcacta aactgtcgat acgtacagta taggattttt atacagtgca
     3061 cggaagtggt gacggtgtaa ctctttctct taaattgacg attaatccgt cagttgaatg
     3121 caaattcaga taaagaacca ggggcagcgt gatcactgcc cctggtcatt aaccgagaag
     3181 gtcttttgcg gtgagttctg tcagcgatga gagtttgacg tttgctaata caaaacgtgg
     3241 atcattttgc gcttctggcg caggaacgac gatggaacgc atgcgggctg ctttagaggc
     3301 gatcatgcca tttaccgaat cctccagcgc tacgcaggtc agtgggtcaa cgcccagttt
     3361 tgctgcgcag tcgagatata cttgcggatg cggcttgctg taaggcagtt tttcggccga
     3421 ggcgagggca tcgaaactgt cgcgtaagtc aaacatggtc aacacttttt ccagcatatg
     3481 tagtggtgac gcggaggcca gtcccaccaa taaaccttgt tctttgcata acgcaacggc
     3541 ttcgcgcacg cctggtaata atggacgtgt ctcttcaacc agtgaaatgg cacgggcaat
     3601 aacccgttct actacttcct gacggcttgg cccattccac ggttgccggg cgtaccaaag
     3661 atcgaccacc atatcgatgc gtaaacctaa ggtgtccggc agctcgttac gacgggagat
     3721 atccaccccc aggcttgcca tcacatccag ttcggctcga tcccataaag gttctgagtc
     3781 gataagtaat ccatccatat caaaaattgc agcaagaatc tgacgcgggg ttgacattac
     3841 aacctctcct ttagagcatt atgtgtaagg tattaagccg tgcttatgat taagcgccta
     3901 tcatatcgcc tttaaagaat gggcgaaaac ggatatctac aggtaaacta agtgaaacga
     3961 cgcgtcttaa tcaaggagaa ctcatgacgt atcaacaagc tggacgcatt gctgttttga
     4021 aaaggattat gggttgggtg atttttatac ctgcattgat ctctacattg atttctttgc
     4081 tgaaatttat gaatacccgg caggaaaacc aggaaggcat taatgcggtc atgctcgact
     4141 ttactcatgt catgatcgat atgatgcagg cgaatacgcc ttttttgaat ctgttctggt
     4201 ataactcccc gacgcccaat tttaatggtg gcgtgaacgt tatgttctgg gtgattttca
     4261 tcctgatttt tgttggactg gcgttgcagg attccggtac ccggatgagt cgccaggcgc
     4321 gcttcttgcg ggaaggtgtt gaagatcaac tcattctgga aaaagccaaa ggggaagagg
     4381 ggctaacgcg tgaacaaatt gagtcacgta ttgttgttcc gcaccatact attttcctgc
     4441 agtttttctc actgtatatc ctgccggtta tttgtattgc tgcaggttat gtgttctttt
     4501 ctctgcttgg gtttatttaa tcaaaaggcc gcacggagcg gccattatct tcaacccata
     4561 tcacgcagct aataatctat ccagtgactg ctgagcaata accaaatgct gaccgccaaa
     4621 taatcttgca cgatttaaca gcgtgtagag ttggtaaacc ggttgacgtt caaggaaatc
     4681 ggcaggtagc ggggatactg actgatagcc gtcatagatt tgtggcggct gttcagtatg
     4741 cagcggtaac atcgccaggt cgcactctcg gtcaccccag tagcaggccg ggtcgaaaat
     4801 gtacgggcca tccggaccca gcgcacagtt gccggaccat aaatcgccgt gcaacagaga
     4861 aggctgcggt tgatgtgagg cgagacgctg ctgaatatgc tcgacgatgg catcgatatt
     4921 gccgaaagcg atcccttttt ctgctgccag ttccaactgc cagccaatcc gttgttcagc
     4981 aaaaaacgtt gaccagcgac gttgccaggt gttgggctgt ggagttgtgg agagcgagtt
     5041 atcaaaatcg agtccaaatt gtggttggtc gctccattga tgtaaacgcg caatttgctg
     5101 accaagaata aatgcgctat gcgcatccag cggacgaggt gggagataat ccatcaccag
     5161 aaaactgtag tcacggtcag cgccaactgc ccaaacctta ggcacggtga cggttttact
     5221 acgcgacagt aactccagtt ggtcggcttc ggcggtaaaa ccgggaagca gttccctttc
     5281 atcacatttg acgaaaaagt catgtcctgc atagcgcaaa tgccatgcgg catggacttc
     5341 tccgccaggc agttcattac gcagttcgat ttcgccttca cctaactgct cgctcaaaag
     5401 acgactgatt gcctgccaca tgatgtctct cccatgttgt ctgccagatc ataaggttag
     5461 cgcaagaatg cggtgtaaaa acacgagcta acgcacacca cagactgatt attgcccaaa
     5521 aaccatttat tttctgttgc tattccattc ctcccaggtc gtgatggtga tttcaggatc
     5581 tttgccggtt gcactttggg taagccccga aaccagttct ctaatttctt cttcactttg
     5641 ggtactgata agtccaaaag tgttagtccc cagctcatgg atatttccct catcatccgt
     5701 catggtgagc aaaaaaccgt cgcgagtcag gtaattattc aactcgttaa tttcagtcaa
     5761 tgtatcctca tgcaacatga cggttatgac ataacggaca agatcgccac tggccatagt
     5821 tcaccccttt gttatcacat gacttttgag catagtcgga gaaacgcgtt gccgacaggt
     5881 tggtgatgat tcccccaatg ctgggggaat gtttttgtta gcgggaaagg taagcgtaaa
     5941 ttttttgcgt atcgtcatgg gagcacagac gtgttccctg attgagtgtg gctgcactcc
     6001 ccgcagctac gccaaaacga accatctctt caagagaggc attttctgcc agtttcagtg
     6061 tcatcgcgcc gaccatgcta tcaccagcgc caacggtact ctggcttttc accggtggtg
     6121 gcaccacctg aatgcagttt tcactatcaa cacccagcgc tccttgtgga cccagggaaa
     6181 cgacaacccg tttggccttg ccgctattaa cgatttcctg cgcggctttg cggacatcgt
     6241 ctggctgggt gagttcgcga ttcaccagcg cactgagttc tttttggtta ggcttaacca
     6301 actcgatgtt accaatcgcc agtgctgcac ttaacgcttc gccagaactg tcgatgatgc
     6361 agcggatccc ttggttttgc gcagcggaaa tcagttgggt taatttttcc agcttcacac
     6421 ctggcggcag gcttccgctt atgaccagga tggccccgga ttcaatttcc agaacttgct
     6481 cttcaagctg gcgaaactca tcttcattta atgccgcgcc tggcataaca aaacgatact
     6541 gctcaccgct tgcttccaca tgtacgtgta aattctgccg ggtccagtct ttggcttcta
     6601 cagtagcgac ggggacattt tcatccgcca acagtgaaac caggtgttcg ccggtcgcgc
     6661 cacccgccgg gaagatcgct gtggcactgc ctccaagatg ggcaatggcg cgggcgacgt
     6721 tgatgccgcc gcccccgggt tcgaacaccg gtgcggtaca gcgcagtttt ccttcgggat
     6781 aaatttgcgg ggtaattgtt gcgctatcga gagagggcgc aagtgtcaac gtatagatac
     6841 gtaccatcat ttcctcctat aggctgattt cagtctggca ccgaatcagc ggaaagtgaa
     6901 gaaattaaca atatgattta ttggagcttt atttaaaatt ttgcagataa atatatataa
     6961 ataaaaatct gttttcgcca catgcttatt caaaaaatga aataagaata cattgataag
     7021 ttattcgcgc ctttttataa ttcgttacct ttccagggac gctaatcttt gatccttaag
     7081 aaagtttccg ggaccgcgat ggtctctttt tttgttgtac ggactcttga taaatgaagc
     7141 ttttgaagac agttcctgcc atcgttatgt tggcgggggg catgtttgcg tcactgaatg
     7201 cagctgccga tgattccgtt tttactgtca tggacgatcc tgccagcgct aaaaaacctt
     7261 tcgaaggtaa tctgaacgca gggtatcttg cacaatcagg caatacgaaa agctcctcac
     7321 ttacggctga caccaccatg acctggtatg gacaaaccac tgcctggtcg ctgtggggga
     7381 acgccagtaa tacttcttct aatgatgagc gttcttcaga aaaatatgcg gcgggcgggc
     7441 gtagccgttt taatttaact gattatgact atttatttgg tcaggcaagc tggctgacag
     7501 accgttataa cggctatcga gagcgcgatg tgttgaccgc gggttatggt cgtcagtttt
     7561 tgaatggccc ggtacacagt ttccgctttg aattcggtcc aggtgtgcgt tacgacaaat
     7621 ataccgacaa tgccagtgaa actcagccgc tgggctatgc ctccggtgct tatgcatggc
     7681 agcttactga caacgctaaa tttacgcagg gtgtttccgt ctttggcgcg gaagacacaa
     7741 cgctgaactc cgaaagcgca ttaaatgttg ctattaatga gcattttgga ctgaaggtgg
     7801 cttacaacgt cacatggaac tctgaacctc cagagtctgc accggaacat accgaccgtc
     7861 ggacaacctt atcgctgggc tacagcatgt aaaattatct gccggaccgt gttatgcggt
     7921 ctggctgtct tcgtttgtca ctgctcagcc ccaaatcatt gctgcccaac gtagcaacaa
     7981 catagcgcca cagattgcca gtagcaccac caccatcttc cagtgttttt tatcaaagcg
     8041 tttagtcggc ataccagtgt cctgcatatc tcttgtgcgt gataacggag tgtaacaaaa
     8101 gggacaggca tcaagggatt ttttgttatt acagccgtcg ggtggcgttg tacacctgat
     8161 cactgtttct gcgttttatg aaaaaataaa attatgtatc gatatataaa tatcgtaaat
     8221 agctggttat aaatacgtct ttaattcatc agcactaaaa ctcatgcttt ggctgattac
     8281 atttatagca gcataatatg tttctacagt tttataatga ttatgatgaa tggctatatg
     8341 aacagctgac atgttgtcat gatttttagc agccaacagt gtaaataact gatgttgact
     8401 aaaagaatgt ttttttgcaa aagcacccag cgtcgatata taagataaca cgacgtcttc
     8461 attacccttg ctcatagcga tgtataatgc aggggttccc tgtgctgtag cgccttttaa
     8521 taaatccatg atgttagctt tgctcaactt gtatttaaag gcgatgccat taattttaga
     8581 gaggaaccgt gtgacacaca aagggtgatt attttccatt gcggcgtata atcctggctc
     8641 accatcatgt attttcatgg ctaaaatact catcaccagt tttttgttta aaagctcttg
     8701 tgctatagga gtggttaatg cattcagaaa gatatcgatt atctcatcga atccatacaa
     8761 catggagata taaagtccag gtgtttcgtt agcactttta gtttgcaaca atttaaccag
     8821 attatcttca tgaatgatgt gattatctac cagtgactga atttcttgca taaatatttt
     8881 cacgttgtca gtatgtccgt tcatcatagc caaaaataaa gcaggtgtac caatgctatt
     8941 ctgcgcagtt attatttcaa tgatttcttt ttcagggagt atgggcaact gtctgaataa
     9001 tgtaataagc tgttgagtct ggcctgtggc cattattaac aacattgccg atggatgtat
     9061 aggggcaaat ggcattcgtg tcgcgatacc cgtgaaaata gcccctggaa ctccctcagg
     9121 taataggggc agaatagcaa ttgcacagtt gcgaacatac ctatcagccc aggtctgagc
     9181 atcgggacct gaactgatga acttatcaag cgagagaaag ccccgtgctg tcctgtgggt
     9241 gcctctgacg gcaacgttgg tatcgttcgg gtcgtaaacg gacacaacat aatttaattg
     9301 cccatacgcc gactgcttta ttcgtagcct tactgacaaa gtatgattgt ctatatataa
     9361 aatcgcagac atatgactga cattgtttgc catcattcga cagaagaaaa tatctaaagc
     9421 gaagccaaaa tccttactcg cgataacgcg actccctgca ggaggatggc gaagtaatgc
     9481 tctgaactcc gcgtcccgga ctaaggggac atgctgctgt atggattcaa cggaggagta
     9541 atgtttaatc aggttgacct tgccttttcc atttctcaat gaatcaagtg cgtattgcaa
     9601 tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc gaaaaaaatg cactaccatt
     9661 gagatttact gttggttgta tagttttttc tctatggatt tttttagaca gtttcataca
     9721 atggttacga ttttcggcag ttaactgttc ctgttcaata taatccagta tgcttcgcaa
     9781 atggttagct ttaataggcg tggtttccag taataaggaa atgatttctt ttttatagtg
     9841 taatgtaatg aatatatttt ctgcatggat gggtgaagtg gcaaagttaa tggtaaaaag
     9901 ggcagtggaa taacaatttt gtgatatttt tcgacagttt gcggtaaatt cctgagtaac
     9961 acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga gttggggcgc
    10021 aaattgatta ataggctctg atgaaggcgt tggtatgtcg ctatcgatgt gcattctatg
    10081 aggagactta taatgagttc tgataataat gtcgttttga ctcataatct attcgcctta
    10141 tatatgtctg gaggttttga gtatgatgat taattattat cgccagaacg aaatgtgcac
    10201 gaaaaattgc tataagaatt gtgtcacttt tatcattgtg atatgaccac ttcgtcatta
    10261 atcttatgat cgtttaaata gtaccgccaa atcacgcaac gaactttgct ggattcagtt
    10321 tatgctgctg taaattcgct cgcagtaaac ctttcagacg ctccgtgatg ttatcagttg
    10381 ttctttaagc gttttgctgg tgtactcact acaaacgaat tgcgaatcga atcaatgtga
    10441 aacggaaagg tacaatctcc ctttttttag tgttaacatc gctcaaccgg gtggaaggtg
    10501 agaggttaat gtctttcatt tgtgtacctg attgcgaacc aatttagcat ttgttggcta
    10561 gatggtttcg caatgaactg ttaataaaca aatttttctt tgtatgtgat ctttcgtgtg
    10621 ggtcaccact gcaaataagg atataaaatg cctgttataa ctcttcctga tggcagtcaa
    10681 cgccattacg accacgctgt aagccccatg gatgttgcgc tggacattgg tccaggtctg
    10741 gcgaaagcct gtatcgcagg gcgcgttaat ggcgaactgg ttgatgcttg cgatctgatt
    10801 gaaaacgacg cacaactgtc gatcattacc gccaaagacg aagaaggtct ggagatcatt
    10861 cgtcactcct gtgcgcacct gttagggcac gcgattaaac aactttggcc gcataccaaa
    10921 atggcaatcg gcccggttat tgacaacggt ttttattacg acgttgatct tgaccgcacg
    10981 ttaacccagg aagatgtcga agcactcgag aagcggatgc atgagcttgc tgagaaaaac
    11041 tacgacgtca ttaagaagaa agtcagctgg cacgaagcgc gtgaaacttt cgccaaccgt
    11101 ggggagagct acaaagtctc cattcttgac gaaaacatcg cccatgatga caagccaggt
    11161 ctgtacttcc atgaagaata tgtcgatatg tgccgcggtc cgcacgtacc gaacatgcgt
    11221 ttctgccatc atttcaaact aatgaaaacg gcaggggctt actggcgtgg cgacagcaac
    11281 aacaaaatgt tgcaacgtat ttacggtacg gcgtgggcag acaaaaaagc gcttaacgct
    11341 tacctgcagc gcctggaaga agccgcgaaa cgcgaccacc gtaaaatcgg taaacagctc
    11401 gacctgtacc atatgcagga agaagcgccg ggtatggtat tctggcacaa cgacggctgg
    11461 accatcttcc gtgaactgga agtgtttgtt cgttctaaac tgaaagagta ccagtatcag
    11521 gaagttaaag gtccgttcat gatggaccgt gtcctgtggg aaaaaaccgg tcactgggac
    11581 aactacaaag atgcaatgtt caccacgtct tctgagaacc gtgaatactg cattaagccg
    11641 atgaactgcc cgggtcacgt acaaattttc aaccaggggc tgaagtctta tcgcgatctg
    11701 ccgctgcgta tggccgagtt tggtagctgc caccgtaacg agccgtcagg ttctctgcat
    11761 ggcctgatgc gcgtgcgtgg attcacccag gatgacgcgc atatcttctg tactgaagaa
    11821 caaattcgcg atgaagttaa cggatgtatc cgtttagtct atgatatgta cagcactttt
    11881 ggcttcgaga agatcgtcgt caaactctcc actcgtcctg aaaagcgtat tggcagcgac
    11941 gaaatgtggg atcgtgctga ggcggacctg gcggttgcgc tggaagaaaa caacatcccg
    12001 tttgaatatc aactgggtga aggcgctttc tacggtccga aaattgaatt taccctgtat
    12061 gactgcctcg atcgtgcatg gcagtgcggt acagtacagc tggacttctc cttgccgtct
    12121 cgtctgagcg cctcctatgt gggcgaagac aacgagcgta aggtaccggt aatgattcac
    12181 cgcgcaattc tggggtcgat ggaacgtttc atcggtatcc tgaccgaaga attcgctggt
    12241 ttcttcccga cctggcttgc gccagttcag gttgttatca tgaatattac cgattcacag
    12301 tctgaatacg ttaacgaatt gacgcaaaaa ctatcaaatg cgggcattcg tgttaaagca
    12361 gacttgagaa atgagaagat tggctttaaa atccgcgagc acactttgcg tcgcgtcccg
    12421 tatatgctgg tctgtggtga taaagaggtg gaatcaggca aaattgccgt tcgcacccgc
    12481 cgtggtaaag acctgggaag catggacgta aatgaagtga tcgagaagct gcaacaagag
    12541 attcgcagcc gcagtcttaa acaattggag gaataaggta ttaaaggcgg aaaacgagtt
    12601 caaacggcgc gccctaaccg tatcaatggc gaaattcgcg cccaggaagt tcgcttaaca
    12661 ggtctggaag gcgagcagct tggtattgtg agtctgagag aagctctgga gaaagcagaa
    12721 gaagccggag tagacttagt cgagatcagc cctaacgccg agccgccggt ttgtcgtata
    12781 atggattacg gcaaattcct ctatgaaaag agcaagtctt ctaaggaaca gaagaaaaag
    12841 caaaaagtta tccaggttaa ggaaattaaa ttccgtcctg gtacagatga aggcgactat
    12901 caggtaaaac tccgcagcct gattcgcttt ctcgaagagg gtgataaagc caaaatcacg
    12961 ctgcgtttcc gcggtcgtga gatggcgcac cagcaaatcg gtatggaagt gcttaatcgc
    13021 gtgaaagacg atttgcaaga actggcagtg gtcgaatcct tcccaacgaa gatcgaaggc
    13081 cgccagatga tcatggtgct cgctcctaag aagaaacagt aaggccttca agtagcactg
    13141 tctgtggagc cttcgggctt cacaggcttt gttcgccttt gtttcgttta ttaacaatgc
    13201 gaagtggaag ttattaaaat gccaaaaatt aagaccgtac gcggtgctgc taagcgcttc
    13261 aaaaaaaccg gtaaaggtgg ttttaagcac aagcacgcta acctgcgtca cattctgacc
    13321 aaaaaagcga ccaaacgtaa acgtcacctg cgtccgaaag ccatggtttc caaaggcgat
    13381 ctgggcctgg taatcgcgtg cctgccgtac gcataagccg ttaacgtttt taacttttta
    13441 attagaatat agatacagga gagcacatat ggctcgcgta aaacgtggtg ttattgcacg
    13501 cgcacgtcac aagaaaattt tgaaacaagc taaaggctac tacggtgcgc gttctcgcgt
    13561 ataccgcgtt gccttccagg ctgttatcaa agctggtcag tatgcttacc gtgaccgtcg
    13621 tcaacgtaag cgtcagttcc gtcaactgtg gattgcgcgt atcaacgcag cagcacgtca
    13681 gaacggtatt tcttacagca aattcatcaa tggcctgaaa aaagcctctg ttgaaatcga
    13741 ccgtaagatc ctggctgata tcgcagtatt cgacaaagta gcgttcaccg ctctggttga
    13801 aaaagcgaaa gcagctctgg cataagccag ttgaaagagg gagctagtct ccctcttttc
    13861 gtttcaacgc catcaaaaca ttgactttta tcgccgtagc cttttcaata aaggtctttt
    13921 gaagagtacc aaaaggtaac gcaagcaatg aatgctgcta ttttccgctt ctttttttac
    13981 tttagcacct gaatccagga ggctagcgcg tgagaagaga aacggaaaac agcgcctgaa
    14041 agcctcccag tggaggcttt ttttgtatgc gcgttttgaa aaataaaaag ccagtctatc
    14101 aggcgtaatt gtcgcagcca gtctggatac ggccagtgcg gagaaaccgg agcgtactta
    14161 agtacgtgag gatttcgagc actgcccggg accaaaatgg caagtaaaat agcctgatgg
    14221 gataggctct aagtccaacg aaccagtgtc accactgaca caatgaggaa aaccatgtca
    14281 catctcgcag aactggttgc cagtgcgaag gcggccatta gccaggcgtc agatgttgcc
    14341 gcgttagaca atgtgcgcgt cgaatatttg ggtaaaaaag ggcacttaac ccttcagatg
    14401 acgaccctgc gtgagctgcc gccagaagag cgtccggcag ccggtgcggt tatcaacgaa
    14461 gcgaaagagc aggttcagca ggcgctgaat gcgcgtaaag cggaactgga aagcgctgca
    14521 ctgaatgcgc gtctggcggc ggaaacgatt gatgtctctc tgccaggtcg tcgcattgaa
    14581 aacggcggtc tgcatccggt tacccgtacc atcgaccgta tcgaaagttt cttcggtgag
    14641 cttggcttta ccgtggcaac cgggccggaa atcgaagacg attatcataa cttcgatgct
    14701 ctgaacattc ctggtcacca cccggcgcgc gctgaccacg acactttctg gtttgacgct
    14761 acccgcctgc tgcgtaccca gacctctggc gtacagatcc gcaccatgaa agcccagcag
    14821 ccaccgattc gtatcatcgc gcctggccgt gtttatcgta acgactacga ccagactcac
    14881 acgccgatgt tccatcagat ggaaggtctg attgttgata ccaacatcag ctttaccaac
    14941 ctgaaaggca cgctgcacga cttcctgcgt aacttctttg aggaagattt gcagattcgc
    15001 ttccgtcctt cctacttccc gtttaccgaa ccttctgcag aagtggatgt catgggtaaa
    15061 aacggtaaat ggctggaagt actgggctgc gggatggtgc atccgaacgt gctgcgtaac
    15121 gttggcatcg acccggaagt ttactctggt ttcgccttcg ggatggggat ggagcgtctg
    15181 actatgttgc gttacggcgt caccgacctg cgttcattct tcgaaaacga tctgcgtttc
    15241 ctcaaacagt ttaaataagg caggaataga ttatgaaatt cagtgaactg tggttacgcg
    15301 aatgggtgaa cccggcgatt gatagcgatg cgctggcgaa tcaaatcact atggcgggcc
    15361 tggaagttga cggtgtagaa ccggttgccg gtagcttcca cggcgtggtc gttggtgaag
    15421 tggttgagtg tgcgcagcat ccgaacgctg acaaactgcg tgtgacaaaa gtgaatgtcg
    15481 gcggcgatcg cctgctggac atcgtctgcg gtgcgccaaa ctgccgtcag ggcctgcgtg
    15541 tggcggtagc gaccattggt gctgttctgc cgggtgattt caaaattaaa gcggcgaaac
    15601 tgcgcggcga accgtctgaa gggatgctgt gctccttttc tgagctggga atttctgacg
    15661 accataacgg cattatcgaa ctgcctgcag atgcgccgat tggcactgac atccgcgaat
    15721 acctgaaact cgatgacaac accatcgaaa tcagcgtaac gccaaaccgt gccgactgtt
    15781 taggtatcat tggtgttgcg cgtgacgttg ccgtgctgaa tcagctgccg ctggttgaac
    15841 cggaaatcgt tccggttggt gcgaccatcg acgacacgct gccgattaca gtcgaagcgc
    15901 cggaagcctg cccgcgttat cttggccgtg tggtaaaagg cattaacgtt aaagcgccaa
    15961 ctccgctgtg gatgaaagaa aaactgcgtc gttgcgggat ccgttctatc gatgcagttg
    16021 ttgacgtcac caactatgtg ctgctcgaat tgggccagcc gatgcacgct ttcgataaag
    16081 atcgcattga aggcggcatt gtggtgcgga tggcgaaaga gggcgaaacg ctggtgctgc
    16141 tcgacggtac tgaagcgaag ctgaatgctg acactctggt catcgccgac cacaacaagg
    16201 cgctggcgat gggcggcatc ttcggtggcg aacactctgg cgtgaatgac gaaacacaaa
    16261 acgtgctgct ggaatgcgct ttctttagcc cgctgtctat caccggtcgt gctcgtcgtc
    16321 atggcctgca tactgatgcg tctcaccgtt atgagcgtgg cgttgatccg gcactgcagt
    16381 acaaagcgat ggaacgtgcg acccgtctgc tgattgacat ctgcggtggt gaggctggtc
    16441 cggtaattga tatcaccaac gaagcaacgc tgccgaagcg tgcaaccatc actttacgtc
    16501 gtagcaaact ggatcgcctg atcggccatc atattgcgga tgagcaggta actgacattc
    16561 tgcgtcgtct cggctgcgaa gtgaccgaag gcaaagacga gtggcaggca gttgcgccga
    16621 gctggcgttt cgacatggag attgaagaag atctggtcga agaagtcgcg cgtgtttacg
    16681 gctacaacaa catcccggat gagccggtac aggcaagcct gattatgggt actcaccgtg
    16741 aagctgacct gtcgctcaag cgcgtgaaaa cgctgctcaa cgataaaggc tatcaggaag
    16801 tgatcaccta tagcttcgtt gatccgaaag tgcagcagat gatccatcca ggcgttgaag
    16861 ccttactgct gccaagcccg atctctgttg aaatgtcagc aatgcgtctt tctctgtgga
    16921 ccggcctgct ggcaaccgtg gtgtacaacc agaaccgtca gcagaaccgt gtgcgcattt
    16981 tcgaaagcgg tctgcgtttc gtaccagata ctcaggcacc gttgggcatt cgtcaggatc
    17041 tgatgttagc cggtgtgatt tgcggtaacc gttacgaaga gcactggaac ctggcaaaag
    17101 agaccgttga tttctatgat ttgaaaggcg atcttgaatc cgttctcgac ctgaccggta
    17161 aactgaatga ggttgagttc cgtgcagaag cgaatccggc actgcatccg gggcaatccg
    17221 cagcgattta tctgaaaggt gaacgtattg gttttgttgg ggttgttcat cctgaactgg
    17281 aacgtaaact ggatcttaac ggtcgcactc tggtgttcga actggagtgg aacaagctcg
    17341 cagaccgcgt ggtgcctcag gcgcgcgaga tttctcgctt cccggcgaac cgtcgtgaca
    17401 tcgcggtggt ggtcgcagaa aacgttcccg cagcggatat tttatccgaa tgtaagaaag
    17461 ttggcgtaaa tcaggtagtt ggcgtaaact tatttgacgt gtaccgcggt aagggtgttg
    17521 cggaggggta taagagcctc gccataagcc tgatcctgca agataccagc cgtacactcg
    17581 aagaagagga gattgccgct accgtcgcca aatgtgtaga ggcattaaaa gagcgattcc
    17641 aggcatcatt gagggattga acctatggcg cttacaaaag ctgaaatgtc agaatatctg
    17701 tttgataagc ttgggcttag caagcgggat gccaaagaac tggttgaact gtttttcgaa
    17761 gagatccgtc gcgctctgga aaacggcgaa caggtgaaac tctctggttt tggtaacttc
    17821 gatctgcgtg ataagaatca acgcccggga cgtaacccga aaacgggcga ggatattccc
    17881 attacagcac ggcgcgtggt gaccttcaga cccgggcaga agttaaaaag ccgggtcgaa
    17941 aacgcttcgc ccaaagacga gtaatctgat ctaactaaaa aggccgctct gcggcctttt
    18001 ttcttttcac tgtcgaagag tcaccgtaaa atcaacgcca tgacacttca gcagaacgga
    18061 taccatgctg acacttgccc gccaacaaca gcgacaaaat attcgctggt tattatgcct
    18121 gtcagttttg atgctgctgg cgcttctctt aagcctttgc gccggtgaac aatggatctc
    18181 gccaggtgac tggttttctc ctcgtggcga actgttcgtc tggcaaattc gcctgccacg
    18241 tacgctggct gtattgctgg ttggtgcggc gctggctata tccggcgctg taatgcaggc
    18301 gttgtttgaa aatcctctgg cagaacctgg actacttggc gtctctaacg gcgcaggcgt
    18361 ggggcttatc gccgcggtat tgcttgggca agggcaactc cccaactggg cgctagggct
    18421 gtgtgcgatt gctggggcgc ttatcatcac tttaatactc ttacgtttcg cccgtcgtca
    18481 tctttcgacc agtcggttat tgctggctgg cgttgcatta gggattatct gtagcgcact
    18541 aatgacgtgg gctatctact tttccacctc tgttgattta cgtcagctga tgtactggat
    18601 gatgggcggt tttggcggcg tagactggcg gcaaagctgg ctgatgctgg cattgatccc
    18661 catgttgttg tggatctgtt gtcagtccag gccgatgaat atgttagcac ttggcgagat
    18721 ctcggcgcgg caactgggtt tacccctgtg gttctggcgc aatgtgctgg tggcagcgac
    18781 cggctggatg gttggcgtca gtgtagcgct ggcgggtgct atcggcttta ttggtctggt
    18841 gatcccacat attcttcggt tgtgtggttt aaccgatcat cgcgcattac ttcccggctg
    18901 cgcgctggca ggggcgagcg cattgctgct ggccgatatt gtagcgcgcc tggcattagc
    18961 tgccgcagag ctgcctattg gcgtggtcac cgcaacgttg ggtgcgccgg tgtttatctg
    19021 gttattgtta aaagcaggac gttagccgca aaaagacggt ctatgattaa aagctaagat
    19081 ttttactgac cacacccagg agaaacgatg caagattcca ttctgacgac cgtagtgaaa
    19141 gatatcgacg gtgaagtgac cacgctggag aagtacgccg gtaatgtgct gttgattgtc
    19201 aatgtcgcct caaagtgtgg cttaacgccg caatatgagc agttggagaa tattcagaaa
    19261 gcctgggccg atcgaggttt tgtggtgctg ggattcccgt gcaaccagtt tctggaacaa
    19321 gaaccgggca gcgatgaaga gattaaaact tactgtacca ccacatgggg ggtgacgttc
    19381 ccgatgttca gtaagattga agttaatggc gaaggacgcc atccgctgta tcaaaaattg
    19441 atttccgcag cgccgaccgc agtcgcgccg gaagagagcg gattctatgc ccgtatggtc
    19501 agcaaaggtc gtgcaccgct gtaccctgat gatattttat ggaattttga gaaattttta
    19561 gttggcaggg acggattggt catccagcgt ttttccccgg atatgacgcc ggaagatccc
    19621 attgtgatgg aaagcattaa actggcgttg gcaaaataat gtctattgtg atgcagttac
    19681 aggatgttgc ggaatctacc cgcctggggc cgctttctgg cgaggttcgg gctggggaga
    19741 tcctgcacct ggtggggccg aatggcgcgg gtaagagtac cttactggcg cgaatggccg
    19801 gaatgaccag cggtaaggga agcattcagt tcgcggggca accactggaa gcatggtccg
    19861 caacaaaact cgcgctgcat cgtgcctatc tttcacaaca gcagacgccg ccgtttgcaa
    19921 tgccggtctg gcactacctg acactgcatc agcacgataa aacgcgtacc gaactactga
    19981 atgatgtcgc aggggcgctg gctcttgatg acaaactcgg tcgtagcacc aatcaacttt
    20041 ccggcggtga atggcaacgc gtacgtcttg ctgcggtggt gctgcaaatc acaccacaag
    20101 ccaatcccgc aggccaattg ctgcttcttg atgagccgat gaacagtctt gatgttgcgc
    20161 aacaaagtgc gttagacaaa attctgagcg cgctgtgtca gcaaggactg gcgattgtga
    20221 tgagcagtca cgatctcaac cacacattgc gtcatgcgca tcgggcgtgg ttgctaaaag
    20281 gtggaaaaat gctggccagt ggacgcagag aagatgtgct cacgccgcca aatctggcgc
    20341 aggcctatgg gatgaatttt cgccgtctgg atatcgaagg tcacagaatg ctgatttcga
    20401 ccatctgatt tcattagtat cttgaaaatc gggtaattac gacgctaaat taatgcaaga
    20461 ataaaaaaca gaggaaccgt acggaatgcg tttctgcctt attttgatca cagcactgtt
    20521 tctggccggg tgtagccacc ataaagcacc gccgccaaat gccagacttt ctgattcgat
    20581 taccgttatt gccggtttga acgaccagct acaaagctgg catggcacgc cgtatcgtta
    20641 tggtggcatg acgcggcgcg gtgtggactg ttcgggattt gtggttgtga cgatgcgcga
    20701 tcgtttcgat ttgcagctgc cccgagaaac caaagaacaa gcctctatcg gcacgcaaat
    20761 tgataaagac gagttgctgc ctggtgacct ggtctttttc aaaacgggtt ccggacaaaa
    20821 tggtttgcat gtaggtattt acgataccaa caaccaattt atccacgcct ctaccagcaa
    20881 gggagtgatg cgttcctcac ttgataatgt ctattggcag aaaaatttct ggcaggcgag
    20941 acgaatctag tatgtcgaaa attggacgtc gatgatacat cgccgtccat ttactccaga
    21001 atacagtagt attttgcaga caaaaattcc gtaaatggat tattcgttta taatcaggat
    21061 aatctaaaaa aaggacccct gaaaaagtgg ttaacatgga acaaatgaaa gttactcctt
    21121 ctgtaattgt tatgtcttat ggcctcatcc cttttagccg gatactgaaa aacatccttc
    21181 gagagggacg gttaccatga agattttttt ggagaatctt tatcattctg attgctattt
    21241 cctgccgata agagataacc agcaagtcct tgtcggcgtt gagttaatta cccatttctc
    21301 cagtgaagat ggcacggtgc gtatcccgac gagtcgggtt atagcgcaac ttaccgagga
    21361 gcaacattgg caattatttt cagagcagtt ggaattacta aaatcatgcc aacatttttt
    21421 tattcaacat aaactctttg cctggcttaa tttaacgcca caagtggcta cattattatt
    21481 agagagggat aattacgcag gcgaactatt aaaatatccg tttattgaac tgttgattaa
    21541 tgagaattac ccgcatctca atgaaggaaa agataatcgc ggtcttttat ccttatcgca
    21601 agtgtatccc cttgtgttag gtaatttggg agcaggcaac agcacaatga aagcggtttt
    21661 tgatggtctt ttcacccgcg taatgctgga taaaagtttt attcagcaac aaatcacgca
    21721 tcgctccttc gaaccgttta ttcgtgcgat tcaggcgcaa atttcccctt gttgcaattg
    21781 catcatcgcg gggggcattg atacgccaga aatattagca cagattaccc ccttcgattt
    21841 ccatgctctc cagggatgcc tgtggcctgc cgttcccata aatcagataa cgacgttggt
    21901 tcagcgataa ccctcctgtt tgctggtgtt taagacgaga gtaaccgtct acactatcaa
    21961 acaggaggct ctatgaccct gtcttttatt acccgctggc gcgatgaatt gccagaaacc
    22021 tatacagcac tttcccctac gcctttaaat aatgcccggc tgatttggca taataccgaa
    22081 ctcgctaaca cgctgagtat tccatcgtcg ctgtttaaaa atggcgcagg tgtctggggc
    22141 ggcgaaaccc tactgcctgg tatgtcacca ctggcgcagg tttacagtgg tcatcagttc
    22201 ggcgtctggg cgggccaact gggtgatggg cgcggcattt tacttggcga acaactgctt
    22261 gctgatggca ctacaatgga ctggcatctg aaaggtgctg gcctgacgcc ttattcgcga
    22321 atgggtgatg gacgggcggt attacgttcg acgatacgag aaagtctcgc cagtgaggcg
    22381 atgcattatc tgggcattcc gacgacccgc gcgttaagta tcgtcaccag cgattcgcca
    22441 gtgtatcggg aaacggtgga gccaggcgcg atgctgatgc gtgtggcacc aagtcatctg
    22501 cgctttggtc atttcgaaca tttttactat cgccgcgagc cggataaggt tcgtcagttg
    22561 gctgactttg ccattcgtca ttactggtca catcttgaag atgatgagga caaataccgt
    22621 ctctggttta acgatgttgt cgcacgtacc gcatcgttaa ttgcccaatg gcagacggtc
    22681 ggctttgcac atggggtgat gaataccgac aacatgtcgc tgctgggact gacgcttgat
    22741 tacgggccgt ttggttttct taatgattac gaacccggtt ttatttgtaa tcactcggat
    22801 catcaaggac gttacagctt tgacaatcaa cctgctgtcg cgttgtggat tttacagcgt
    22861 ctggcgcaga cattgtcacc atttgttgcc gtagatgccc tgaatgaggc cctggacagc
    22921 tatcagcagg ttttgttgac gcattatgga caacggatgc ggcagaaact gggctttatg
    22981 acggagcaaa aagaggataa cgcgctactg aatgaattat tcagtctgat ggcgcgagag
    23041 cgcagcgatt atacccgcac atttcgcatg ctgagtctga ccgagcagca cagcgcggcg
    23101 tcaccgctac gtgatgagtt tattgatcgt gcggcatttg atgactggtt tgcccgttat
    23161 cgagggcgtt tgcaacaaga cgaggttagc gatagtgagc gtcagcaact gatgcaaagc
    23221 gttaaccccg ctctggtatt gcgcaactgg ttggcgcaac gggcgattga agcggcagaa
    23281 aagggtgata tgacggaatt gcaccgcctg catgaggcgt tgcgaaatcc tttcagcgac
    23341 agagatgatg actatgtcag tcgtccacct gactggggta aacggctgga agtcagctgc
    23401 tcgagttaaa ggctacttgg tcaacagcag cttgccagcc tgagttttac ggagcagata
    23461 ttcttgcccg tcatgatcga taatcagttt gccatccgga cctaacagtg tctggctgga
    23521 aattcgccga ataggctgcg gggaagcggt cttgtgattg gcatccgttt caggcgtgag
    23581 ttttctgcta tccgtataac gcataaaaat taataataat tgatataaca atgattctta
    23641 ttatcaatat tatggctgac cagagcaagc agtaatgtgt tgagaagaca acaagttgcg
    23701 cagaaatgcc catcccatca ggaatgggca cgcattcaga agcgggtatc taccgcagag
    23761 gctagttttt cgaccaggcg ttcggtatct tcccagccca gacacgggtc ggtaatggat
    23821 tgaccgtaag tgagcggctg accgccgacg attttttgcg ttccttcgcg caggaaactt
    23881 tccgccataa ttccagcaat cgccgtagag ccattgcgga tttgctgaca aatatcctca
    23941 caaacttcta actggcgacg gtgctgcttc tggcagttac cgtggctgaa atccaccacc
    24001 agatgttcag gtaaatcaaa ctcgtgcagc gtatcgcagg ctgcggcgat atcatcggca
    24061 tgataattcg gttttttgcc gccacgcata ataatgtggc catacgggtt gccgctggtc
    24121 tgatagatgg tcatctgacc atttttgtct ggcgagagga acatatggct ggcgcgggct
    24181 gcgcggatag catccacagc aatccgcgta ttgccatcgg taccattttt aaaacctacc
    24241 ggacaggaga gtgccgaagc catttcacgg tggatctgac tttcggtagt acgtgcgcca
    24301 atcgcgcccc aactgattaa atcagcaata aactgaccgg tcaccatatc gaggaactca
    24361 gtcgcggttg ggacgcccag ctcatttacc tgtaaaagta atttgcgcgc cagctccaga
    24421 ccgtgattta tccgatagct gccgtttaaa tctggatcgg agattagtcc tttccagccg
    24481 acaacagttc gtggtttttc aaaataggtg cgcattacga tttccagccg tgactggtac
    24541 tggttgcgca gcgactgcag acgggtggcg tactccattg cagcggtgag atcgtggatc
    24601 gagcaggggc caataatgac caacagtcgc ttatcttcac cattgagtat tttttcaatt
    24661 ctgcggcggg agtcggtgac atgggtggcg acgccaggcg ttacgggata ccgtagcgcg
    24721 agttcggcgg gcgttaccag gctctcaata cgcgcagtac ggagttcgtc agttctgttc
    24781 attacaagtc tcagaaattt gttgcttcta cggcaggact gccgggaagt gatgcgcatc
    24841 acgataaacc aatcgctgct gatttcaagt gcgaggtgtg tcacacctcg ccgggtggtt
    24901 agcatgataa caaaaaaata agctaatgca ctagttctct agtacattcg gcgactaagg
    24961 cccatgatat cgaggatctt ggtggcaatc tcttctaccg aataattggt actgttaatc
    25021 cacgggatct gatttttacg gtacaaggct tccacttccg cgacttccat cctgcactga
    25081 cgaagcgagg catagcgact gttctcccga cgttcctcgc gaatcgccgc cagacgttcc
    25141 gggtcgatag tcaggccgaa caatttatgc tgaagcggtt tgagcgacgc gggtagcacc
    25201 agattatcca tatcgtcggc aataaagggg tagtttgccg cgcggatacc aaattgcatt
    25261 gccagataca gactggtggg ggttttacca cagcgagaaa caccgagcag gatcacctga
    25321 gcctgatcca gattgcgcaa cgaaatgccg tcatcgtggg cgagggtgta atcaatcgcc
    25381 gcaatgcgcg catcatattt attgagatta ttagggttaa ggccatgggt acgatgagca
    25441 atcggcgttg gatccagttt catctcttgt tgtagcgggg caaccagcgc ctgaacgata
    25501 tcctggcaaa aaccttcact ttgcaagatg atggcgcgaa tttccggcaa cacgatggag
    25561 tagaagacca gcggacgcac gcctgtctgg tgataaattg cgtcaatctg atccttcact
    25621 gcccgtgcac ggctctcatt ttcgacaaac ggcagcgtga tgctgctgat agtgacggga
    25681 aattgtgaca ttactgcgtg tcctaatacc tccgcagtta ttgccgtacc atcagaaata
    25741 taaaaaacgt ggcgatcaac agcattatcc attttgttct tcccgtgatg cagacataat
    25801 tctatgaaag cataaattaa aaacgcacag aagcgtagaa cgttatgtct ggtttataaa
    25861 atgaaccttc aattttattt tttatgaaaa cagcatttca tttttatggt ttcgtttata
    25921 ccgatggttt atgtggaaat tgtcgaaaag agcagatttg cgcaacgctg ggatcagtct
    25981 taacaagtaa aaaaatatat ttgcttgaac gattcaccgt ttttttcatc cggttaaata
    26041 tgcaaagata aatgcgcaga aatgtgtttc tcaaaccgtt catttatcac aaaaggattg
    26101 ttcgatgtcc aacaatggct cgtcaccgct ggtgctttgg tataaccaac tcggcatgaa
    26161 ggatgtagac agggttgggg gcaaaaatgc ctccctgggt gaaatgatta ctaacctttc
    26221 cggaatgggt gtttccgttc cgaatggttt cgccacaacc gccgacgcgt ttaaccagtt
    26281 tctggaccaa agcggcgtaa accagcgcat ttatgaactg ctggataaaa cggatattga
    26341 cgatgttact cagcttgcga aagcgggcgc gcaaatccgc cagtggatta tcgacactcc
    26401 cttccagcct gagctggaaa acgccatccg cgaagcctat gcacagcttt ccgccgatga
    26461 cgaaaacgcc tcttttgcgg tgcgctcctc cgccaccgca gaagatatgc cggacgcttc
    26521 ttttgccggt cagcaggaaa ccttcctcaa cgttcagggt tttgacgccg ttctcgtggc
    26581 agtgaaacat gtatttgctt ctctgtttaa cgatcgcgcc atctcttatc gtgtgcacca
    26641 gggttacgat caccgtggtg tggcgctctc cgccggtgtt caacggatgg tgcgctctga
    26701 cctcgcatct tctggcgtga tgttctccat tgataccgaa tccggctttg accaggtggt
    26761 gtttatcact tccgcatggg gccttggtga gatggtcgtg cagggtgcgg ttaacccgga
    26821 tgagttttac gtgcataaac cgacactggc ggcgaatcgc ccggctatcg tgcgccgcac
    26881 catggggtcg aaaaaaatcc gcatggttta cgcgccgacc caggagcatg gcaagcaggt
    26941 taaaatcgaa gacgtaccgc aggaacagcg tgacatcttc tcgctgacca acgaagaagt
    27001 gcaggaactg gcaaaacagg ccgtacaaat tgagaaacac tacggtcgcc cgatggatat
    27061 tgagtgggcg aaagatggcc acactggtaa actgttcatt gtgcaggcgc gtccggaaac
    27121 cgtgcgctca cgcggtcagg tcatggagcg ttatacgctg cattcacagg gtaagattat
    27181 cgccgaaggc cgtgctatcg gtcatcgcat cggtgcgggt ccggtgaaag tcatccatga
    27241 tatcagcgaa atgaaccgca tcgaacctgg tgacgtgctg gtcactgaca tgaccgaccc
    27301 ggactgggaa ccgatcatga agaaagcatc tgccatcgtc accaaccgtg gcggtcgtac
    27361 ctgtcacgcg gcgatcatcg ctcgtgaact gggcattccg gcggtagtgg gctgtggtga
    27421 tgcaacagaa cggatgaaag acggtgagaa cgtcactgtt tcttgtgccg aaggtgatac
    27481 cggttacgtc tatgcggagt tgctggaatt tagcgcgaaa agctccagcg tagaaacgat
    27541 gccggatctg ccgttgaaag tgatgatgaa cgtcggtaac ccggaccgtg ctttcgactt
    27601 cgcctgcctg ccgaacgaag gcgtgggcct tgcacgtctg gaatttatca tcaaccgtat
    27661 gattggcgtc cacccacgcg cactgcttga gtttgacgat caggaaccgc agttgcaaaa
    27721 cgaaatccgc gagatgatga aaggttttga ttctccgcgt gaattttacg ttggtcgtct
    27781 gactgaaggg atcgcgacgc tgggtgccgc gttttatccg aagcgcgtca ttgtccgtct
    27841 ctctgatttt aaatcgaacg aatatgccaa cctggtcggt ggtgagcgtt atgagccaga
    27901 tgaagagaac ccgatgctcg gcttccgtgg cgcgggacgc tatgtttccg acagcttccg
    27961 cgactgtttc gcgctggagt gtgaagcagt gaaacgtgtg cgcaacgaca tggggctgac
    28021 caacgttgag atcatgatcc cgttcgtgcg taccgtagat caggcgaaag cggtggttga
    28081 ggaactggcg cgtcaggggc tgaagcgtgg tgagaacggg ctgaaaatca tcatgatgtg
    28141 tgaaatcccg tccaacgcct tgctggccga gcagttcctc gaatatttcg acggcttctc
    28201 aattggctca aacgacatga cgcagctggc gctcggtctg gatcgtgact ccggcgtggt
    28261 gtctgaactg ttcgatgagc gcaacgatgc ggtgaaagca ctgctgtcga tggcgattcg
    28321 tgccgcgaag aaacagggca aatatgtcgg gatttgcggt cagggtccgt ccgaccacga
    28381 agactttgcc gcatggttga tggaagaggg gatcgatagc ctgtctctga acccggacac
    28441 cgtggtgcaa acctggttaa gcctggctga actgaagaaa taaaataaat ccccggcggc
    28501 gttttgtcgc cgggtttatg tgatccccga agatgaaact tattcaatct cttcacagac
    28561 atcctgcgtt aatcgccgca taatatcttt ccgtaacaaa aacttttgta ttttacctga
    28621 ggcagtgcgc ggtagttttt cgattaccac gatatgttca ggatatttat attttgcgac
    28681 ccgtttacgg ctaaaaaaag ccaccacctc ttccagcgat aatgaatgat gcggcgcttt
    28741 cagcacgaca taagcgcatg atcgttcacc taaacgttca tcgggcattg caaccacaca
    28801 ggcatcgtga attttaggat gctgcaataa aatatcttcc acttcacggc tgctaatatt
    28861 ttcgccgccg cggacaataa tatctttttt gcgtccggta atttttatat agccagcctc
    28921 atccatacgg cagagatcgc cgctgtaata ccagccttct tcatccaggg cacgggcggt
    28981 taattcaggt tcatcaaaat atcccataaa cacattgggg ccacgcgagg cttcttcacc
    29041 ttcgcaacct ggcggtaagg tcttgcgtgc gtcatcgacc actttaatct ctacacctgc
    29101 ggcagcgtaa ccatcggtgt gcataaagcg cgacaaagga tcatcgagat tcaccaccgc
    29161 atgcggcgaa ctttctgtgg aaccataaac acttaataat ttaatgccgc gctgctggca
    29221 ttcacgcgcc acttttttgg ggattgtggt accgccgcaa agaaagaagc gcagcgctga
    29281 aaggtctgcg ggttgtttct ctagtaaatt caaaagatca tagacaaacg gcgttgcgcc
    29341 gagcatacag gagcaacgct gctgctcaag cagcgcgaga cacgcatcag gagtgaaaat
    29401 atctaacaac acgctgcgag cgccaattaa gaatggtgcc gttacgccat gcagaaagcc
    29461 cgttgcgtga ccaagtggcg caggcatcat aaagacatcc tgccaggtta gattcagccg
    29521 cgcgcaataa gcccgctcac tggcgagaat attgttatgc gtaagcatca cgccctttgg
    29581 cagaccctcg gttccggagg taaacagcac cgcagctaat tcatcgccgt gggtcgttat
    29641 cgccgtggtc agtggggtat tgtcggcgat aatctgactt aatgagaggg aagaggtggc
    29701 gggagccagt ttgtccacgc cgacaatttg ttgtagttgt ggaagctgat tttgcagcgg
    29761 caggattaaa tctaccggac gcgtttgttt aaacaacgtc ggtgtaaaga acatttttgc
    29821 ctgacactta ttaagcaccc ataccagttc tgcttcccgc caggaaggca acagcggtac
    29881 ggaaaccgca ccgattttca ggcaggcaag atagataacg gtaaattcac accagcctgg
    29941 cagttgaaat gctatgcgat cgcctgattc aatacccttc gctaacatcc agtttgccag
    30001 acagctcgcg gcgtgatcga gcgcgctata ggtgtacgat gctccatgat tatcgaccac
    30061 ggcaattttg tctggcatcg cacgagcggt ctgctgccag taatcggcca gcgaagcatc
    30121 gccccatagc ccttgctgac gatacgccgc acgacgttgt tcgttaaacg ttaatgtcac
    30181 tttcattttg gactctcgaa acagaacatc aggcccgaga tgcgggcctg tgggatgcat
    30241 gattagccgt agcgaaaatc aataccgaag gtgcctgcgg gatattgcca ctgttcgaga
    30301 atagtgttac cgcacagcac ccgacaggtg ccgcactcca gacaaccggc ggaatcaaaa
    30361 tgaatgtttc ctgcgtcatc ctgcttataa agtccggcag ggcaggcttt cattaattta
    30421 cggaattcat tgatatcggg atttgccgcc aaaatgatat gcgggtggcc ctcatcaaca
    30481 tggaatttat tgacgcctaa tttgatgtcg acgttaaccg tagcgttctg gctcatagcg
    30541 cggttgctcc cttaatgcca tctttcagca agttgatcag cccaattttc ttcgcgtgtc
    30601 ccatgatcat tttgcgtacc ggctggttag gcttaccgtc aatggtgaac atctcgttca
    30661 tgatgtcggc taccattcgt gggtattggc taaacaggcg cgggttttcc atcagcgccg
    30721 ggatcttgcg aaaatgctgc atatcacgca tgacgcagct ttgttccagc tcacgtttgt
    30781 attgcgccag actgctggcg gagaaatccg cgcgttcttt ggcggcgatc actgttgtgg
    30841 cggcagcctg agccgatgca atggctaaat ccatgccgcg gaccgtaaaa cccaaattca
    30901 ggcagaagcc tgcggcgtca ccaacgatca tcacgccatc gttaaccagt tgcggtacca
    30961 tcgccagacc gccttctggc accatatgcg cggaatattc aagcagtttg ccgccgctaa
    31021 tcagcgggcg aatggcgggg tgttgtttaa aatcttccag catttgcggc acgctttttt
    31081 gcgcatgggc gatatcagcc aatccacaaa ccagccccaa ggatatggaa tccttgttgg
    31141 tatagagaaa tcccccgccc atcaggccgt cagaagggga accggcaaac agccaggcgg
    31201 caccttcctc gcccgtaata ttaaagcgat cgttgatctg ttctggtgtg aggccaataa
    31261 cctctttaac accaacagcg taatgatgcg gatcggaagc gggaaccatt cccagcgagc
    31321 ggccaagcat cgagttaacg ccatcggcta aaatcaccac attggcttcg agaatatcat
    31381 ccccggcctg gactccagtg accttgtttc cttcacgaac caacgcatcg acgcgaactc
    31441 ccgggataaa ctgtgcgccc gcctgctcgg cttgttccat caaccacggg tccagacgat
    31501 tacgcaatac ggtataagat gcgtgttgcg gaacatctgg ttgctcgcgg tgaaaatcga
    31561 gggtaacggc gctctcttcg gttaagaagg agattttctc gcgtgtgacc ttgcgttcta
    31621 ccggcgctga tgctgcaaag cctggaatga ttgcttcaag tgtgtgggca taaagacgcc
    31681 cgccggtcat gtttttacat ccggcactgt cgccgcgttc tatcaccagg atatccagcc
    31741 cggctcgcgc catgaccagt gcggcaacgc taccagcaac gcccgcaccg accacaatgg
    31801 catcaaattt gtcatccgac atgcgttatg ctcccttccc gctgcgttta cagcgggaga
    31861 tgtggttgaa aggggaatta gcggctcaac tggctaatca gggcagggac gactttgtag
    31921 atatcgccca ccagaccgta gtcggcatag ttgaagattg gcgcattttt atctttattg
    31981 atggcgacaa tcacttttgc gccgttgccg ccaaccatat gctggatctg cccggagatc
    32041 cctagcgtca ggtagagatc ggatttcagc aacacgccgg agacaccgat ataacgttca
    32101 cgctccatcc agttctcgcc ttcggcaatt ggacgtgaac agccgacttc agcattcagc
    32161 accgccgcca gttcgtggac cattttcagg tcatcctgcg ctgccagacc acgaccgacg
    32221 ccaaccacac gttttgcttt gctcaggtcc acgctgcttg cggctttcgc acggcgttcg
    32281 cgacagagaa tttcatgacg cggagcaacg taaggtaccg tttctgtcgg gcactgatga
    32341 gaggtatcac tggtgcacgg ttcctgaaca ccgggtgcaa gggtaatgat cgccagcgag
    32401 ctgttgatct tttcctgagc gaacgctaac ccgccataca tccggtgttc ggcgcaaatg
    32461 tgaccatcga caatatccac cgccgtggca tcgttcacca gcgccgcatt cagttgcaca
    32521 cttaaccgtg ctgccagcgc tttaccacgt ttcgtcgcgg ccagtagcaa catagcgggg
    32581 tgtttatctt tcagcagagc agcaatgctt tcggcgtaat tttcagtgcg ttgcagcgcg
    32641 tcgttttgct caagaacata aatacatttt ggaccataag gcataactgc ctgcgcctgg
    32701 tcggtatttt gtacaatggc atacacctgt tggccccatt gctgagcgcc gccaaacagt
    32761 tcagcataac gttcaggatt atcgctaaag acccagacgc tgtttaattg actcatcgtt
    32821 tcttctccgt aggcttcagt tcagggcttt cttcagatgt tccgccagct cggcaatggc
    32881 ctccggcgaa tcgttatcga gaatgatgtg cttacgttct gtttgcggcg gtacgcgaat
    32941 gccggtcagt tcagcaagtg gtgcgctctg gctccagcca atatcacttg cctgccactg
    33001 atttaccggt tttttacccg cgccgagaat ggctttcatc gaaggaatgc gtggcacgtt
    33061 aatatcggag gtgacgcaga gcacggctgg aacagagagt tcaataactt caacgtcatc
    33121 ttcaagcgtg cgttcaatca ccagtgtatt gccctgacgc tgaatagcac tcactgcatt
    33181 aatcaccgga agttgcagaa tttctccgac cagcaagcca acctgctggg cataaaggtc
    33241 gccggaacct ttaccaaaga tcagtaaatc gaagccgatc ttttcaactg ctgccgccag
    33301 cgcttttgcg gtatcgagag gcagtgcatg ctcaagttgt gcatcctgca ccatatacag
    33361 gctgtgcggc ccgcgggata gcacgtcttt gcgcactttc gagttctgca acaatgagcc
    33421 gccaacggtc agcgcggcta tctcatcgtc atctgttgca agctggcttg cagcttcaat
    33481 ggcattgaga tcgaactggc tgattttggc gtcggcattg tcgaaattca gggtgtattc
    33541 tggagtgaca acaatgtcct gttcttcagg caccagctta aagcaggtta ttattttcat
    33601 gatttctcct gtgagtcgca tacggaacca tctccgatgg aaggtaaaaa tggcctgaaa
    33661 atagtgtgcg atagcggtgc ggggttttcg acggagcaag ctgttttcct tgccaacaat
    33721 cttgcaaaaa aaatgcgatc gctaaaacaa aagtgcaatt tgcggcaata tagttattcc
    33781 atgaccgtac aatcttgcat tcagtgtaaa taatcttgtt tagctctcgc taaacgcggt
    33841 tgtataaccg caatacacgc cgtattttat ccggcatata ttgtttttgc ttgctcatgg
    33901 aatagggagg ttgtcatgta tcaacgctgt tttgataatg ccagtgaaac gctgtttgtc
    33961 gctggtaaaa cgccacggct ttcacgtttt gcatttagcg atgatccaaa atgggagtct
    34021 ggacatcacg ttcatgacaa tgaaaccgag ctgatttacg tcaagaaagg ggtggcaagg
    34081 tttaccatcg attcttcgtt atatgtcgcg catgcagatg atatagtggt gatagaacgc
    34141 ggcaggctgc atgcggtggc ctctgacgtt aacgatccgg caacgacgtg tacttgtgcg
    34201 ctgtacggct ttcagtttca gggggctgag gaaaatcatc tactgcaacc gcattcttgt
    34261 ccggtaattg ccgcggggca gggaaaagaa gtcattaaaa ccttatttaa tgagctaagt
    34321 gtgattttgc cgcaaagtaa aaatagccaa acatcttcgt tatgggatgc atttgcctat
    34381 acattagcaa ttctttacta cgaaaacttt aaaaatgctt atcgttcgga gcaggggtat
    34441 attaaaaaag atgttctgat aaaagatatt cttttctatc tgaataataa ttatcgcgaa
    34501 aaaatcactt tagaacagtt atcgaaaaaa tttcgtgcca gcgtcagtta tatttgccat
    34561 gaatttacca aagagtatcg tatttcccct attaactatg ttattcaacg gcgtatgacg
    34621 gaagcaaaat ggtcactcac taatactgaa ttatcacagg cagagatctc ctggcgtgtg
    34681 ggttatgaaa atgtcgatca ctttgccaaa ctgtttttgc gccatgtcgg ctgttcgccc
    34741 agcgattacc gcaggcaatt taaaaactgt tttgcggaac aagaaatcct atctgaattt
    34801 cctcaaccgg taagtcttgc cggataaatc tggcggcgca gtttgttaag aagctgcgcc
    34861 gcctgcagat tatttgttct gatagtcttt caggatctgc cgacctgcta cgtaaatcat
    34921 aatttcgtct gtaccgccgc caatacgttc acaacggaca tcacgccaga agcgggagac
    34981 gcgcgcctca tcggtatagc ccagaccgcc catgatttga atcgcatcat caatgacttc
    35041 cattgcggta cgtgcacagt acagttttgc cagcgccgcg ctggtgcgca gtgattgatg
    35101 ctgatcggct tgccatgcca ctttcagcac catgttgcgc atgttgtcaa tcttaatcgc
    35161 catcagcgcc agtttttcct ggatcatctg gttatgacca atgggtttac caaaagcgat
    35221 acgctggttg gcatagcgag cggcatcttc aaaggcacac tcggcaaagc cggtgctgcg
    35281 cgcggcgttg atcaggcgct ccatctcaaa gttgtacatc acattgagga aacccattcc
    35341 ttcttcgccc accatgtcgc tctcttcaac ttccacgttg tcgagataga cttcgcaggt
    35401 gctgagcata tgccagccga ttttatgcag cgggttaatc ttaatgccgg gcttactgga
    35461 gtcgacccac cacagggtga aggctttttt gggatctttc ggttgcggat cgcgcgccag
    35521 caccagcata tacgggtact ctttcgcacc ggtaataaag gttttctgtc cgttgatgta
    35581 aaccttgccg tttttacgcg tgtaagtggt agtggcactg ttgttatctg agccagcacc
    35641 tggttccgtc aacgccaggg cataggcggg atcacccgtt tccagggtgc tttctgccgt
    35701 tttacgtagc tgctctgcag aaccgaaacg gcgcatactg tgaatacatt gaccgttggt
    35761 aattaaaaat gccggagcac cgcattttga cacttccatc agcgccagca tttgggtgac
    35821 gtaatcagca gggataccac caaattcttc cggcacgcca agcatggaaa taccgttatc
    35881 cgccagtgcc cgcataaact cacgcggata tgtcccgttt tgatcgcagg tgcggaaata
    35941 ctcttccgga aagttagtcg taatcagttc gcgaatactg gccagcagca gttcttgttc
    36001 ttcagttaaa gaaaaatcca tcgtgatact cctgttaatg agccgcttca ggcaggacaa
    36061 aacccatcgc cgcatcgata aataatcttt cgtccatcag tttgagttct ggcgaaatca
    36121 ctggggtgaa atccattttg tcgagaatat ctttttgtaa atcgacgcca ggggcgattt
    36181 cgattaaatg cagtccgtct tctttcagcg tgaatacggc gcgctcagtg atataacgaa
    36241 catccagccc tcgctcgaga gcgatttttc cgctaaaagt aatttccggt agttcccgaa
    36301 taaatttctt cacccgtcct tcctggacga tattcaattt gccatcggca atttctgttt
    36361 ttaaactgcc cgcagttaac gtgccgcaga aaataatttt cttcgaggtg gcactgatat
    36421 caataaatcc accggtaccc atgattttgc cattgaattt atgcacgccg acgttaccgt
    36481 gctggtcgac ttcagcaaaa ctcaaataac aaacatccag accaccaccg tgataaaaat
    36541 caaactggga cgtcatatcc agaatggcac gggtattcac gttcgcgcca aaggcgatac
    36601 cctgtgaagt aattccacca atcggacctg tttctaccgt cagaataaag tcatcagcac
    36661 aaccttcttc ccgggcgacc aggccaatgc cgtcagcaat accgacgccg acattcccca
    36721 ccgcgccttt acgcatttcg aataatgcac gccgtgcaac taatttacgt tggtttaggg
    36781 gcaggctaag tttggtgctg tcatcaaggg tgaagtcacc agaaataaag cggttaaccg
    36841 gcgcaccgcc atatagttgg ctttgatccg gatcgaccac cacaatatcc accagataac
    36901 ccggaatacg gacagattta ggatgcagtg tggctttctt aaccattttc tgcacctgca
    36961 tcatcacaat accgccgtta ttgtgcaccg cctgggcaat aaccaatgcg tcgagataca
    37021 tcacctcatc ttcaaaagtg gcgtagcctt cactgtcgca ggtggtagcg cgaatgaaag
    37081 caatatctgg cgcaatcgct ttgtaataga gatattcttt gttatcaaac tcgaccagtt
    37141 taatcaggtc ttctttagtg acttcattca gtttgccgcc ttgctggcgt ggatcgacaa
    37201 atgtcccgat gccaatatca ctaataatac caggctggtg ggctgcggcg gcgcgtaagg
    37261 tttgtgtaag tacaccttgt ggatagttat aagcaataat tttattttgt tctgcgagat
    37321 cagaaatacg cggcgattgt ccccagtgac cgcataatgc ccatttcacc agaccttctt
    37381 gcgccagcgg actaataccg cggtcggcgc gatcgccaag ccctgttgga ctaataatcg
    37441 ataaattacg tggcgtttga gtctgtttat atttatcagc aagagcagta attaacgtgg
    37501 tggcttccag aataccgccg ccagcgccta acacacaaag tgttgcttcg tcgggaatat
    37561 aattcaccgc ttcctgtgcc gacaggaccg gcactcgtcc attaatacga ggtggtttta
    37621 caggtttcat tgttaattcc tgattcaggt gtgattttcc ctacgccaga tggataatta
    37681 gcgcacagag actcacgaaa tatttttatt aaatacggtt gaatatattt ttttagttcg
    37741 gcgggggagg atattcccgc cgaaatatta ttgcttatgc ctgatgtaaa atagttaata
    37801 ctgtgcgcaa atcatttacc gagatttgcc ctggcgcaga ggcttttttt accgcaccaa
    37861 aagttgccgc cgagccaaat acttcaccag ccagacgaga aattacgcca gtttttgcca
    37921 ttgacatcgt gatgattgga cgatcggcat actgctcctg catctccagg gtcgcggcaa
    37981 gcaacgccag cacatcgctg gtactttgcg gcatcagcgc aatcttagga atatcggcgt
    38041 cgaaggactg cattttgcgc agacgggcaa tgatttcttc ggcttccggc gttttatgga
    38101 agtcatggtt ggacatgaca actttcacat catgcgcgtg ggcgtaggcg acggtttctt
    38161 taacctgatc atcgccggta aataactcca gatcgatcat atcaaccagg ccgctgtcga
    38221 tggctgcacg attgagagca atataagcct cggtggaaat cgcctgctcg ccgccttctt
    38281 tggcactgcg gaaggtaaac agcagcggtt tttctggcat ggtttcacgg agaatttttg
    38341 ccgccgccat gacagactcc acattggaga ggtcggcaaa gtggtccaca cgccattcca
    38401 gaatatcaaa gtccgcttca cgataggcga gagcttcgga tttcactcgg gcgatatctt
    38461 tcgccatcag cgagacgatg attttaggtg cgcccgtacc aatgacgaga tcttttacag
    38521 ttacggtttt cattttttac cctttctgca cgcggtcagc ctgtcaggca ccgaacccca
    38581 tgacctgttt aacatattcc agagggaaat ctttgccagt ccataacgtg aactgttcag
    38641 ccccttgcca caacaacatg ccgtatccat caatcgtttt gcaaccagct tgttgcgcct
    38701 gctgcaataa cttcgtcata tgcgggttat acacgcattc agtgaccagc agtcccggat
    38761 gtaacagact gatatcatta accaatgatt tattctcaag gggtttcata cccacttttg
    38821 tgccattggt taaaatgtcg gcggaagcca gggcttcagc aaaggcttgc tgatcggcga
    38881 gatcggtaac cgtgacgaca caatcggtgt tttcgttaac ccgctgcgcg aaggcgaggg
    38941 ctttatcgaa gaacttatcc cgacggttaa agagtttaat ttcttttaaa ccttcaattg
    39001 ccccctgcgc gccaatcgcc gttgaggcac ctccggcccc taacagcacc attgttttgc
    39061 ctttgatatc aaaaccgctc tctttaatgg cgcgaatatg gcccgtgccg tcggtgttat
    39121 agccgcgcag gtagccatca tcattaacga tggtgttgat ggcacccacc agtttggctg
    39181 ctggcgttaa ttcatcaaca tattcacacg ccagttgttt gttcggcatt gatacaccag
    39241 ttccgcgcat ttttagagct tttaatcctt caattgctcc gggaaagcta tcgttatcca
    39301 cttcgaaggc catataggta aatggcaatc ccgctttttc taaggcttta ttctgcattt
    39361 caggcgataa actgtggcgg ataggataag ccatcaaccc aatcaattcg tattttgcgg
    39421 taacatccat aattaactcc tttaacctct atgcttaatt gtgtttaacc gggtgctaaa
    39481 tttgcgctcg ccaaaccgca catcattctc aggaataata aaaacgcggt aatagcggat
    39541 aaaaacaata attgcggtaa tcagggccag cagcgcgaaa gtaaaatcga gaacaatgat
    39601 atattgcagg ccgatgttcg acagataacc ggtaatcagc ggaataacaa agttagccag
    39661 tccacccatc atcatataaa tactggtgac tttggctttg ctttttggga aaaactctga
    39721 catgaccgaa acaccgagct gtaaaatgcc gccagctgct gagaaaccga taacaaaggc
    39781 gccggcatta cacaccagtg gagaagggta caggtagata atggctgctg ttattgttgc
    39841 cagtgcagag ttaaatacat tagcccagat gggccggacc atttttttca gtagtgcggc
    39901 aaaaataaag acacagacca gcgagcccat actgtaataa gagatggttt ttaatgcctc
    39961 agcttctgac ataccagcaa aagccatcgc atatttgggc atccacacca caatcacata
    40021 aaaggtcgag aatgcggcta caccgaacag taccgatgaa acaccttcca gccagactaa
    40081 cggtttgctg ttcatttgcg gtaattcatt agctacgctg gcgtccacca actggctggg
    40141 gaatttgctt ttcaacagca tcagcgtgat cagtacaaac agaatacccg gaataatcag
    40201 cccatagccg taccagatat tattgagcaa catatagctc accagcattg ggtagaacat
    40261 ttgcccaaat gacaccatcg ctttaaccag tatgaccgcc gaaccagagg ctttcggaaa
    40321 gcattccatg agcgcggggt agccacccgt atccagcgct gagttagcga tacctacgca
    40381 cactgccaga ccgtaggcga gagttaaatt cgggcaagcg ggaataccaa agaagaatag
    40441 cagatacatt attactgcca ttaatatcac cgcccgacga ccaaacttat cggagatcac
    40501 accgaagaat aaaatactga tcaatcgccc caaaccgata ccggaaatta agtaggcaat
    40561 gcccgcgttg tcagtggaaa acttttccgc cagagatgac atattttggg caagcgtaat
    40621 aacactaatg ccgtgcagga agtagctgaa gtaaatacaa agaacagcca ggataaatgg
    40681 cgtgctgaaa gccttatttt gagacatttt cacagctcct tgctgaaaga taatttcgtt
    40741 accgtacgta ttgttctccg acggtttaca taattactat actgaagatg attttccctg
    40801 ggtacatggc ataaagttta tgtttccagg aatattgcat gtcatagttt gtttttgatc
    40861 gcgattttcc ttagttagcg cagaaagagt tatttatccg taagatcttg catgacctga
    40921 aaagaatatt gcattaccca cccggagcga cattttcctt tagcgagtga tgccgcgttt
    40981 ttttgcggct gcgtaagccg ataaacagtg ccagtaaaaa accgatggca gcgatggcgg
    41041 tatcgaacca cataatatcg gcaatacttc tttgggacag atgagccgta atcaacggaa
    41101 tagtaaaggt cgcaatactg cccgcactgt aatagatccc tgtagcttta cctttagcgt
    41161 aagggaaacg ttcagccatt aacgtcaggc caatttgcac aacacctcca gcagaagtaa
    41221 aaccaatgac aaaagaaaat attatcacca cataaaatgt gggatgcagg cagacggtaa
    41281 acagagcaat aaatgagata aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat
    41341 tacgaatgag tggagcggta ataaatacac aaagcagcga acccacggtg tagatactga
    41401 gtagtttgat cgacatagtg tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc
    41461 actggctaac cagataaaac gttgccattg agatatagcc atataaggta taactggcta
    41521 aatcgataat tgaacagcga tgttccgtag agctggtggt tttctttatg acaggtaagc
    41581 gacgacccgg atggggtggg aacgtacaac ggtataaaaa cagagcgtta ataaacataa
    41641 tgcctgcagc aatcataaag gaccaaccga accacagttc agcccacact aacaggctaa
    41701 tgattagcgg taataaaaat tgtccgctgg aaacaaatgc tttaattaaa atattggctg
    41761 tcccaggtga gcgtggaaaa gcttccatca aactgggata agtgcctgca tcgagaaaac
    41821 tgtttgccat tcccgccaga aagccaaaaa cataagcgat aatgatgtta ttggtctgca
    41881 ggatgccaaa aaagaaggcc atatagcagc acatcccgag catgataaaa gggcggcgac
    41941 caaagcgatc ggataataat cctgcaaaaa gcaagacact taatcgacca atgcccagcg
    42001 atgagataac tatcgagaca cccgcggcat tagactgcca aagtgtctcc agcgaggcca
    42061 tattcaggct catcaaaatg acgcccatac catgcaccag gtaattaaaa tacaacccaa
    42121 gtgcggtagg gtaataggga tttttcatag gttctactta gcaaaggtgc tttaaaaagt
    42181 gtagcgatgt tgtgtcagta tccgttcatt attgttgtaa caacgacaat tcctttttgt
    42241 ggagaaatta acaaagccgc tccagatcgt gtgcatataa ttcagcggct accgattgat
    42301 agatcttcca gtcaataaaa ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg
    42361 ggaaaaaacg catggtgttg ttgccgatgc ggttatttat tttctctatg atcgcatgaa
    42421 gatgtttttt tctttgctga cgcatagcca acagttgctc gactacacaa tcaggaatgg
    42481 cacacctgcc attttcccat tgacgccagc tttcactgtc accggtctgg gcaatccagg
    42541 tcgcgcattc atcaatagtc atggcaaaaa tatggcggag tgcctggagt tcataagcgt
    42601 tcatcgtgta atggctgcat gaaataaaca ataaaatata aataacatta tcagcctgct
    42661 gacggcttga agagagtcac agtatcttgt gcaacattat tggaaagagt gaggggcgag
    42721 gtagcgaagc ggaaaaatgt taaaaaaaag cccatcgtgg gagatgggca aagactacac
    42781 acagcaattc gttgtttcac tcaggggatt tccatgctta taaatcaata tgttgattta
    42841 taaccgtgag ctaatagtag gcatacggac tttttgcgtc gatcagattc gtctcattag
    42901 tttaaaaact gtaaagaatt tgcgacaggt actaccagct gaatggcggt aagcaggaaa
    42961 atatggggaa gagcaagcaa atatcaatta gcggtagtta acaatcaggc ggcagagccg
    43021 cctgatgaga aattacttat tcggtttctg gatttcgcca agttcttcga gaatttcttc
    43081 cggttgatcc gtcggcggtg ggacctcttc cacccacgcg gcaaacagac gccaggaaac
    43141 ggctaacaga accggaccaa taaacagacc gatcatcccg aaagcaatca aaccaccaat
    43201 aacgccagag aggatcagga tcagcggtaa atcggcaccc atgcgaatta acattgggcg
    43261 gatgacgtta tccagcgtgc caaccacacc gctccacact aacaataccg ttccccaggt
    43321 ggtatcgcca gtccagtaga gccagataat cgccggaatc agtaccggca acgggccaag
    43381 ctggacaagg caggaaagga tcattaacac cgttagcaaa gttgcataag gtacgccgga
    43441 tacggcgagg ccgataccgc caagcactgc ctgtactaac gccgtcacca ccacacccag
    43501 cgccaccgcg cggatagcct gtgccgccag cagtacggcg gcatctccgc gaacgcctgc
    43561 cagacgggtc gcaaaatggc gaatgccttg tgccacctgt tcaccgcgcc agtacagcag
    43621 ggcactgaag agaagcatca gcgcacaatg caccataaag cgcccgatat gcgccgcttg
    43681 cccaacgaac caggtagtgg tggtgccaat ataagggcgg actttcgcca tgatcgccgt
    43741 cccccccata tccagcaagt tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat
    43801 ggtattaagc cacgccagat cgggtaacgt catgtcaccg ctggaaatgg ctttaattag
    43861 cgggccgctg ccgtcgacga tactgttaac cagcaaagca atagggatga taaacaccat
    43921 cactaataac agcgtcatca ccagaacggc gagagagcgg cggccaaaca tgatcttttg
    43981 caaacgtaac aataccggcc aggtggcgat aaccaccgta ccggcccatg caaagccgag
    44041 aataaagggt tgaacaatcc acagacatgc cacaatcatg atggctaaaa acagcaccga
    44101 aagcagaatt tgtgcgacat ccctgggctg acgaacattt accataacta cttttcacct
    44161 tttttgtgcg tcaaatcgtc gacgcgacgt gaacacgcga aactcacctg cataatgatg
    44221 agcaatttca gcggttttta acaggccgat tctgccctta attctgatgg gcgtgttaga
    44281 aaaaaatgtg gtacaacaac tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt
    44341 tgcatatgct gcggctacgt tcgttcaccc cagttactta ctgttgtaag ctcctggagt
    44401 tcactcgctt gccgccttcc tgcaactcga aatccataga gtaaataaac atcacctttc
    44461 tgtgcgctat tgcagccgcc tgaaagaaca gtgtatcgac ctacatcaca gaccgcagga
    44521 aagggtcaat ataatgattc cacagatttc ccaggcaccc ggcgtcgttc aactggtgct
    44581 taattttttg caagagctgg agcaacaagg ttttaccggc gatacggcga caagttatgc
    44641 tgatcgtctg acaatgtcga ccgacaacag tatttaccaa cttctccccg atgcagtggt
    44701 atttccgcgt tcaaccgcag atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta
    44761 ttcatcgctg atctttaccc cccgcggcgg cggcaccggc actaacggtc aggcgctcaa
    44821 ccagggtatt attgttgata tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga
    44881 agagggctgg gtgcgcgttg aggccggggt gataaaagac caactcaatc agtacctgaa
    44941 accgttcggc tacttttttg caccagaact ttcgaccagc aaccgggcaa cgctcggcgg
    45001 gatgatcaat accgatgcat ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca
    45061 cgtacttggc ctacgtgcgg tattgttggg gggcgatatt ctcgatacgc aacctttacc
    45121 cgtcgagctg gcggaaacgc tgggtaaatc caataccaca atcgggcgaa tttataacac
    45181 ggtttatcaa cgttgccgtc agcaacgcca gttaatcatc gacaacttcc ccaaacttaa
    45241 ccgctttctt accggttacg atctgcgtca tgtctttaac gatgagatga ccgagttcga
    45301 tctgacgcgc attctgacgg gttcagaagg gacgctggcc tttattaccg aagcgcggct
    45361 ggatattacg cgcttgccta aagtgcgccg tctggtgaat gtcaaatatg actcttttga
    45421 ctctgcgctg cgtaacgcgc cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt
    45481 ggactcaaaa gtgctgaatc tggcgcggga agatattgtc tggcattccg tcagcgagtt
    45541 gattaccgat gtgcctgaca aagagatgct cgggctgaac attgtggaat ttgctggtga
    45601 tgatgaggcg ctgattgacg agcgggtaaa tgcactctgt gcgcggttgg atgagctgat
    45661 cgccagtcat caagcaggtg tcatcggctg gcagatgtgc cgtgagctgg cgggggttga
    45721 acgtatctat gcaatgcgca aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc
    45781 taagccaatt ccgtttgctg aggatacctg cgtaccgccg gaacatctgg ccgattatat
    45841 tgctgaattt cgcgcgctgc tcgacagcca cggcttaagc tacggtatgt tcggtcacgt
    45901 cgacgcaggt gtcttgcatg tccgtccggc actggatatg tgcgatccgc aacaagagat
    45961 tttgatgaag caaatctctg atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt
    46021 gtggggcgag cacggcaaag gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga
    46081 actttttgca gaactgcgca aagtgaaagc ggcatttgac ccgcataacc gactcaaccc
    46141 agggaagatt tgcccgccag aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa
    46201 gcgcggtaca ttcgatcggc agatccccat tgcggtacgc cagcagtggc gcggtgcgat
    46261 ggagtgtaac ggcaacggtt tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc
    46321 gatgaagatc acccagaacc ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga
    46381 atggctgcgt ttgttggcgg atcgcggcgt tgatccactc aaactggaac aagaactgcc
    46441 tgaatccggc gtcagcttgc ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa
    46501 taaaggcgaa tatgacttct cacacgaagt caaagaggcg atgtcgggct gtctggcctg
    46561 taaagcttgt tcgacccagt gccccatcaa aattgatgtg ccggagtttc gctctcgttt
    46621 tctgcagctc tatcacaccc gttatttacg cccgctgcgc gaccacctcg tcgctacggt
    46681 cgagagctac gcgccgctga tggcacgcgc gccgaaaacc tttaacttct tcattaacca
    46741 gccgctggtg cgcaaactct cggaaaaaca tatcggcatg gttgatttgc cgctgctgtc
    46801 ggtcccctcg ctacaacaac aaatggtggg gcatcgctcg gcaaacatga cgctggaaca
    46861 gcttgaatct ctcaatgcag agcagaaagc gcgcacagtg ttggtggtgc aggacccctt
    46921 taccagctat tacgatgcgc aagtggtggc ggattttgtc cgtctggtcg aaaaattagg
    46981 tttccagcct gtgttactgc cattttcgcc aaatggcaaa gcccagcata ttaaaggttt
    47041 tcttaatcgt tttgcgaaga cggcgaaaaa gacggcggat ttcctcaacc gtatggcgaa
    47101 gctgggtatg ccaatggtgg gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata
    47161 taaactggcg ctgggcgagg agcgtggcga gtttaacgtc ttactggcga atgaatggct
    47221 ggcaagcgca cttgagtcac agccagtggc tacagtcagc ggtgaatcat ggtatttctt
    47281 tggtcactgt accgaagtta ccgccttgcc gggtgcgcca gcacaatggg ccgcgatatt
    47341 tgcccgtttt ggcgcgaaac tggaaaatgt cagcgtgggt tgctgcggca tggcagggac
    47401 ttacggacat gaagcgaaaa accataaaaa ttcgctcggg atctatgagt tatcctggca
    47461 tcaggctatg cagcgactgc cgcgtaaccg ctgtctggcg accggatatt cctgccgtag
    47521 tcaggtcaaa cgggttgaag gcacgggggt acgccatcct gtgcaggctt tactggagat
    47581 tattaaatga tatggaaacg aaaaatcccc ctggaagcac tgaatgccat gggtgaagga
    47641 aacatggtgg ggttactgga tattcgcttt gaacatattg gtgatgacac ccttgaagcg
    47701 acaatgccag tagactcgcg gacaaagcag cctttcgggt tgctacatgg aggtgcatcc
    47761 gtggtactgg ccgaaagtat cggttccgtt gccggttatt tatgtaccga aggtgagcaa
    47821 aaagtggttg gtctggaaat caatgctaac cacgtccgct cggcacgaga agggcgggtg
    47881 cgcggcgtat gcaaaccgtt gcatctcggt tcgcgtcacc aggtctggca gattgaaatc
    47941 tccgatgaga aagggcgttt gtgctgttcg tcacgattga cgaccgccat tttgtgacgt
    48001 tgtcaccaga aaagtgtgac gcatattgct tttggtcatt atctgcacag taaacggtgg
    48061 tatactgtgc aggtcttgtc taaaagcgag gatgctatgt ctactcaact tgatcccacc
    48121 cagctggcca ttgaattttt acgccgtgac cagagcaatc tttcacctgc ccaatatctt
    48181 aaacgtctga aacaactaga acttgaattt gctgacctgc ttactctctc ttctgctgaa
    48241 ttaaaagaag aaatctactt tgcctggcgt ctgggcgtgc attgatcctg acttaatgcc
    48301 gcaagttcgc caaatcgacc tggattaacc tccgtccagg ttcgatgacc ttcttaatac
    48361 tgcttatcta cccgcctgat attgttcaaa aataatacta atcgcagttt gtgttaaaac
    48421 ggcggcttag ctttatgaga ttattcttat cgccccttca agagctaagc cactgagagt
    48481 gccggagata agcgccggat ggggtagaaa cccttaagcc tgtgtcgcac agacttaagg
    48541 gttttcttat ttctgaacgc tgcacttttt tacctatctc ttaaatagtc attttcaata
    48601 caactttttc tttttcatca cagccttaat gctccttaga taataatcat tatctaacaa
    48661 tgagatacct aattcttagc gtgcctgtta acccacacat cagggtctat gcttattaaa
    48721 agcaggcaaa aagcatgttt tacggtaaag cccctgcctt tgctgggttg aactgataat
    48781 cattatcact aacatgctgt tatacgctga aagcgatgaa gtgaggtaaa tcgatggaca
    48841 tgcattcagg aacctttaat ccacaggatt tcgcctggca aggcttaacg ctgacacccg
    48901 cagccgctgt ccacatccgc gagctggtgg caaagcagcc gggtatggtc ggcgtgcgct
    48961 taggtgtgaa gcaaacgggc tgcgcgggct ttggctatgt gctcgacagt gttagcgagc
    49021 cggacaaaga cgatctgctg tttgaacacg acggcgcgaa gctgtttgtc ccgctgcaag
    49081 cgatgccgtt tattgatggc acggaagtcg atttcgttcg tgaaggactt aatcagatat
    49141 tcaaatttca caaccctaaa gcccagaatg aatgtggctg tggcgaaagc tttggggtat
    49201 aggcggtact atgtctcgta atactgaagc aactgacgat gtcaaaacct ggaccggcgg
    49261 cccgctgaat tataaagaag gattcttcac ccagttagcc accgatgagc tggcaaaggg
    49321 gataaacgaa gaggtggtgc gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct
    49381 ggagtttcgt ctaaacgcct atcgcgcatg gctggagatg gaagaaccgc actggttgaa
    49441 agcgcactac gacaagctga attatcagga ttacagctac tactcggcac catcgtgcgg
    49501 taattgtgac gacacttgcg cgtctgaacc tggcgcggtg cagcaaactg gcgcgaacgc
    49561 ctttttaagt aaagaggtgg aggcggcgtt tgagcagttg ggtgttcccg tgcgggaagg
    49621 caaagaggtg gcggtggatg ccattttcga ctcagtttcg gttgccacta cttatcgcga
    49681 aaaactggcg gagcagggaa ttattttctg ttcctttggt gaggcgatcc acgatcaccc
    49741 ggaactggtg cgtaaatatc tcggcaccgt ggtgccgggg aatgacaact tctttgccgc
    49801 gcttaatgcg gcggtagcct ctgacggtac gtttatttat gtgcctaaag gcgtgcgctg
    49861 cccgatggaa ctttccacct attttcgcat taacgcggaa aaaaccggac agtttgagcg
    49921 caccattctg gtggccgacg aagacagcta cgtcagctac attgaaggct gttccgctcc
    49981 ggtgcgtgac agctatcagt tgcacgcggc agtggtggaa gtcatcatcc ataaaaacgc
    50041 cgaggtgaaa tattccacgg tacaaaactg gttccctggc gataacaaca ccggcggtat
    50101 tctcaacttc gtcaccaagc gtgctttgtg cgaaggcgaa aacagcaaaa tgtcatggac
    50161 gcaatcagaa accgggtcag cgattacgtg gaaatatccc agctgcattt tgcgcggcga
    50221 taactccatt ggtgagtttt actcagtggc gctgaccagc ggtcatcagc aagcggatac
    50281 cggcaccaag atgatccaca tcggtaaaaa caccaaatcg accattatct cgaaagggat
    50341 ctctgccgga catagccaga acagttatcg cggcttagtg aaaatcatgc cgacggcaac
    50401 caatgcgcgc aatttcactc agtgcgactc aatgctgatt ggcgctaatt gtggggcgca
    50461 taccttcccg tatgtcgaat gtcgcaataa cagcgcacaa ctggagcacg aggcaacgac
    50521 atcacgtatt ggtgaagatc aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga
    50581 cgccatctcg atgattgtta acggtttctg caaagacgtg ttctcagaac tgccgctgga
    50641 atttgccgtt gaagcacaaa aactcctcgc catcagtctt gaacacagcg tcggataagg
    50701 aataaacatg ttaagtatta aagatttaca cgtcagcgtg gaagataaag ctatcctgcg
    50761 tggattaagc ctcgacgttc gtcccggcga agttcacgcc attatggggc caaacggttc
    50821 gggcaaaagt accttatcgg caacgcttgc cgggcgagaa tattatgaag tgacgggcgg
    50881 cacggttgag ttcaaaggca aagatttgct tgcactgtcg ccggaagatc gtgcaggcga
    50941 aggcatcttt atggccttcc agtatccggt ggagattcca ggtgtcagta accagttttt
    51001 cctgcaaacg gcacttaatg cggtgcgcag ctatcgcggc caggaaacgc tcgaccgctt
    51061 tgattttcag gatttgatgg aagagaaaat cgctctcctg aagatgccgg aagatttatt
    51121 aacccgttcg gtaaacgttg gtttttccgg cggcgagaaa aagcgcaacg atattttgca
    51181 aatggcggtg ctggaaccgg agttatgcat tcttgatgaa tcggactccg ggctggatat
    51241 cgacgcatta aaagtggtcg ccgatggcgt gaactcgctg cgtgatggca agcgctcatt
    51301 catcattgtt acgcactacc aacgcattct cgactacatc aagcctgatt acgttcatgt
    51361 gctatatcag ggacgaattg tgaaatccgg cgatttcacg ttggtcaaac aactggagga
    51421 gcagggttat ggctggctta ccgaacagca gtaacgcgct gcaacagtgg catcacttgt
    51481 ttgaagctga agggacaaaa cgctccccgc aagcacagca gcatttacaa caattgctgc
    51541 gtaccggact gccgacacgt aaacatgaaa actggaaata tacgccgctg gaagggctga
    51601 ccaatagtca gtttgtcagc attgcgggag agatatcccc acagcagcga gatgccttag
    51661 cgttaacgtt agacgctgtg cggctggtat ttgtcgatgg acgttacgtg cctgcgctga
    51721 gcgatgcgac tgaaggcagc gggtatgaag tgagcattaa cgacgaccgt caggggttac
    51781 ccgatgctat tcaggcggaa gtgtttctgc atttgacgga aagcctggca caaagcgtga
    51841 cgcatatcgc cgtgaagcgc ggtcaacggc cggcaaagcc attgctgtta atgcatatca
    51901 cccagggcgt ggcaggtgaa gaggtgaaca ctgcccatta ccgacatcat ctggagctgg
    51961 cggaaggtgc cgaagcaacg gtgatcgaac attttgtcag ccttaatgat gctcgtcatt
    52021 ttaccggggc acggttctct atcaacgtcg cagcgaatgc ccacttgcag catatcaagc
    52081 tggcgtttga aaacccgctc agtcaccact ttgcccataa cgatttgttg ctggctgacg
    52141 atgccaccgc atttagccac agtttcctgc tgggtggcgc agtgttacga cacaacacca
    52201 gtacgcaact caatggcgaa aactgcacgc tgcggatcaa tagcctggcg atgccggtga
    52261 aaaacgaggt gtgtgatacc cgtacctggc tggaacacaa taaaggtttt tgtaacagcc
    52321 gacagttgca caaaactatc gtcagcgaca aaggccgcgc ggtatttaac ggtttgatca
    52381 acgtcgcgca gcacgccatc aaaacggatg gtcagatgac caacaataat ctgctgatgg
    52441 gcaaactggc ggaagtggat acgaaaccgc agctggaaat ctatgcagat gatgtgaaat
    52501 gcagccacgg cgcgacggtg gggcgtattg atgatgaaca gatgttctat ctgcgctcgc
    52561 gcgggatcaa tcagcaggat gcccagcaga tgatcattta cgcctttgct gccgaactga
    52621 cggaagcact gcgtgatgag gggcttaaac agcaggtgct gacccgaatc ggtcaacggc
    52681 tgccaggagg tgcaagatga ctttttccgt cgacaaagtg cgggccgact ttccggtgct
    52741 ttcgcgtgag gtaaacggtt tgccgctggc ttatctcgac agcgccgcca gtgcgcagaa
    52801 accgagccag gtgattgacg ccgaggccga gttttatcgt catggctacg cggcggtgca
    52861 tcgcggtatt cataccttaa gcgcccaggc gaccgagaaa atggagaacg tgcgcaagcg
    52921 ggcatcgctg tttattaatg cccgttcggc ggaagagctg gtgttcgtcc gcggcacgac
    52981 ggaagggatc aatctggtcg ccaatagctg gggcaacagc aacgtgcggg cgggcgataa
    53041 catcatcata agtcagatgg agcaccacgc taacattgtt ccctggcaga tgctttgcgc
    53101 acgcgttggc gcagagctgc gtgtgatccc gctcaatccc gacggtacgt tgcaactgga
    53161 gacgctgcct acgctgtttg atgagaaaac tcgcctgctg gcaattactc atgtctccaa
    53221 cgtgcttggc acagaaaatc cactggcgga aatgatcacg cttgcgcacc agcatggcgc
    53281 aaaagtgctg gtggatggcg ctcaggcggt gatgcatcat ccggtggatg ttcaggcgct
    53341 ggattgcgat ttttacgtgt tttccgggca taaactgtat ggccccaccg ggattggcat
    53401 tctttatgtc aaagaagcct tgctacagga gatgccgccg tgggaagggg gcggttctat
    53461 gatcgccacc gtcagcctga gtgaaggcac tacctggacc aaagcaccgt ggcggtttga
    53521 agccggtaca cccaataccg ggggcatcat tggtcttggc gcggcgctgg agtatgtttc
    53581 ggcgcagggg cttaataaca tagccgagta tgaacagaat ctgatgcatt acgcgctatc
    53641 acagctggaa tctgtaccgg atctcactct ctatggccca caaaacaggc ttggcgttat
    53701 tgcttttaat ctcggtaaac accacgccta tgatgttggc agttttctcg ataattacgg
    53761 cattgctgtg cgtaccggac atcactgcgc tatgccatta atggcctatt ataacgtccc
    53821 tgctatgtgt cgggcgtcgc tggccatgta taacacccat gaagaagtgg atcgtctggt
    53881 gaccggactg caacgtattc accgtctgct gggataacag ggaggcacta tggctttatt
    53941 gccggataaa gaaaagttgc tgcgtaattt tttacgctgc gccaactggg aagagaaata
    54001 tctctacatt attgagctgg gccagcgtct gccagaatta cgcgacgaag acagaagccc
    54061 acaaaatagc attcagggct gccagagtca ggtgtggatt gtcatgcgtc agaatgcgca
    54121 gggaattatt gaattgcagg gcgacagcga tgcggcgatt gtgaaagggc ttattgcggt
    54181 cgtctttatt ctctacgatc agatgacgcc gcaggatgtt gtcaatttcg atgtgcgtcc
    54241 gtggtttgaa aaaatggcac tcacccaaca tctcacccca tctcgttcac aaggtctgga
    54301 agcgatgatt cgcgcaattc gcgccaaagc cgctgcactt agctaaacta aagggacagc
    54361 tttcatccgg ttggctcaca ttgccggatg gtcaatgttt acactgacct gcacaggagt
    54421 gtttcggttt caggtgaaca taagcgccat ccgacgtttc agcgtgagtc tccggcaaat
    54481 acaggaggtt tacaatgaaa cgcgcgtctt tgcttacact cacgcttatc ggcgctttta
    54541 gtgccatcca ggctgcctgg gcggttgatt atccgctacc accaaccgga agccgactgg
    54601 ttgggcaaaa tcaaacgtat acggtgcagg aaggggataa aaaccttcag gccatcgccc
    54661 gacgttttga tactgcggca atgttgatcc ttgaagccaa taacactatc gccccggtgc
    54721 caaaacccgg tacgacgata actattcctt cgcaactgtt attacctgat gcgccgcgtc
    54781 aggggattat cgttaacctt gcagagctgc gcctttatta ttatccgccg ggagaaaata
    54841 tcgtgcaggt ttatccgata ggtattggct tgcaggggct ggaaacgcca gtgatggaaa
    54901 cgcgtgtggg gcagaaaatc cctaacccaa cctggacgcc tacggcaggc attcgtcaac
    54961 gttcgctgga gcgtggcatt aaattaccgc cagtcgttcc tgccggacca aataacccgc
    55021 taggacgtta cgcactgcgc ctcgcgcatg ataatggcga atacctcatt catggcacca
    55081 gtgcgccgga cagcgtcggt ttgcgcgtca gttcagggtg tattcgcatg aatgcaccgg
    55141 atattaaagc cttgttctcc agcgtgcgga cgggaacgcc ggtgaaagtg atcaacgaac
    55201 cagtgaaata ttccgtggag cctaacggga tgcgttatgt tgaagtacat cgaccactat
    55261 cggcagaaga acagcagaac gttcagacaa tgccatacac actgccagca ggctttacgc
    55321 aatttaaaga caataaggct gtagatcaga agttagtcga taaagcgttg tatcgtcggg
    55381 cagggtatcc ggttgcggtg agcagtggag caactcccac agccagcaat gcgccttcag
    55441 tagagtcagc gcagaatggt gaaccagagc aagggaatat gttacgcgcg acgcagtagt
    55501 ggtaaatagc agacaaaaaa atggcgcaca atgtgcgcca tttttcactt cacaggtact
    55561 attacttgcg gtatttagta gccatgttgt ccagacgctg gttagcacga gctgcgtcat
    55621 ctttagcagc ctgaacgtcg gaacgcattg cgttcacgtc gttgctcagc tggtcaactt
    55681 tagcgttcag agtctgaacg tcagaagaca gctgatcgat tttagcgttg ctggagcaac
    55741 ctgccagcag agtagaaccc aggattaccg cgcccagtac cagtttagta gctttcatta
    55801 ttaataccct ctagattgag ttaatctcca tgtagcgtta caagtattac acaaagtttt
    55861 ttatgttgag aatatttttt tgatgggaag gcacttattt ttgatcgttc gctcaaagaa
    55921 gcatcgaacg ctgggtttta gataaaaaaa ttgagagaaa acaggatgct tccatcggat
    55981 tcatcttaga taaagtgaga ttatatagtg aaatccgatt tgatttttta ttgcttctgg
    56041 ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg atatacaaat taattcacaa
    56101 aagcaatatt acaggacgtg aacagatgcg gtgttagtag tgccgctcgg taccagtgca
    56161 ccagaaacca taactacaac gtcacctttg tgtgccagac cgctctgcag agccagttct
    56221 ttacccagac ggtagaaatc atcagtagaa gtgatctctt taaccagctg cggcacaacg
    56281 cctttgctca gtaccaactg atgagccgtt ttttcgttgg tggtcagtgc caggatggtg
    56341 gcatccggga agtatttacg tactgcgcga gcagatttac cgccctgggt agcaaccacg
    56401 atcagcggag catccagttt ttcagcagtt tcaacggcac cacggcatac cgcttcggta
    56461 atgcgcagtt tacggttgtc attgttgaac tcgagacggc tgttcatcac gcggtcggta
    56521 cgttcgcaga tggtcgccat gatagaaacc gcttccagcg ggtatttacc ttttgcggat
    56581 tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg cgtttgcaac gtcaccggct
    56641 tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca gcatctgggt cgcagtgata
    56701 acgactttac gtgcacggat acatttttcg atcatcatct tctgggcgaa gataacttct
    56761 tctaccggga tttctacacc caggtcgcca cgcgcaacca tgatgccgtc agaggcttcg
    56821 aggatttcgt cgaagttgtt gaggccttcc tggttttcga ttttggagat gatgtggatg
    56881 ttttcgccgc cgtgcgcttt caggtgctca cggatttcga taacgtcaga acgcttacga
    56941 ataaaggaag cagcgacaaa gtctacgcct tgttcgcaac caaagatcag gtcctgtttg
    57001 tctttttcag ccagtgctgg cagggcaatg gaaacgccag gcaggttcac acctttgttt
    57061 tcacccaggt cgccgttgtt cagcacttta cagataactt tgttaccttc aatggcagta
    57121 acttccatac cgatcagacc atcgtcaacc agtacggtgt tgccaacaga caggtcagtt
    57181 gtgaaacctt catacgttac cgcaaccatt tcgctgttgc cgataacaga tttatcagta
    57241 gtgaaagtaa aggtctgacc agctttcaga gaaacgtcgt taccgccttc cagtttcatg
    57301 gtgcggattt ccggaccttt ggtatcaagc aggatagcgg cggttttacc agttttgctc
    57361 atcacgttgc gcagattctg aatgcgctga ccgtgttctg catagtcacc atgagagaag
    57421 ttcagacgca taacgttcat gccagcgtcc agcattttag ctaacatctc ttcagattcg
    57481 gttttcggtc cgatggtgca aacaattttg gtctttttca tgacagtctt agtctttaag
    57541 ttgagaagga tgggagaaac ttgctttctg ggcgcactgc cggaaagcga atccggtatt
    57601 acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc gaaaaagcgt gcagaggaat
    57661 gtgtgcttgt gtctcagccc aacgtgagtg cctgtttttg agtcgttgag ttctacagtc
    57721 caaagtgctg aaaccattca agagtcaatt ggcgcgcatt atacgctgaa attaatccaa
    57781 aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg ttagacaata aattgttatc
    57841 aaagatttaa cgttccaggg aaaaggttac gcaaaaactt cgctgtttgc gcaacgtctg
    57901 gtgccggggt tgtcactgtg aagataagtg aaagttcatc cggtgtataa atacatcatc
    57961 gctcaattgc tacagttaaa ataatttatc gcgttatttg tctgattttg tttgattttt
    58021 gtttaattgg ttagattgat atatgctgat agttggcatg ataaataggt ataaagaatg
    58081 ggaaatcgga caaaagagga tgaactgtac cgagaaatgt gcagagttgt cggtaaagtt
    58141 gtgctggaaa tgcgcgatct ggggcaggaa ccaaagcata ttgttatcgc aggcgtatta
    58201 cgtacagcat tagccaataa acgcattcag cgcagtgagc tggaaaagca ggcgatggaa
    58261 accgtcatta acgcactggt aaaatgacca ttcaggatga ggcgataagc ttcatcctga
    58321 tattcttgct tcagttgcgg tccagttaat cattctaaat aacaactgct gaaattatac
    58381 gaatttcgat atgtgtttta atttataacg ataatattat ttttattttc tgcaatgacc
    58441 gtcacaaata attgtcgctt aagttaaatt catttcaaat gataataaat atcagttcac
    58501 ttataatttc ttgctttgta attttaattg ataatgatca atgttaagag cccagaataa
    58561 ccacattatt ttttctccta accataaagg attagtttat cggcgttaaa tattttctca
    58621 tacactatcg ggttagggaa aatatctggt gttggggaca gagtcagcag cgtattagtt
    58681 gaatgcttaa tatcaggctg ctgaattgtc agcactgtgt ttgcgaagag gatgcgaaat
    58741 aaatgaaccc ggttgatcgt ccactattag atattggctt aacgcgatta gaattcttgc
    58801 gtatatcagg aaaaggcctt gctggtctaa caattgcccc tgcgttactg tcacttttgg
    58861 gttgcaaaca ggaagatatt gatagcggca cagtagggtt gataaataca cccaaagggg
    58921 tgctggtgac ccagcgagcg cgatgcacgg gttgccaccg ctgtgaaatc tcttgtacca
    58981 acttcaacga tggctcagta gggacattct tctcccgtat caaaatccat cgcaattatt
    59041 tctttggcga caacggggtt ggctctggcg gcggcctgta tggcgatctc aactataccg
    59101 cggacacctg ccgtcaatgc aaagagccgc aatgcatgaa cgtctgcccg attggtgcga
    59161 ttacctggca gcagaaagaa ggctgtatta ccgtcgatca taaacgttgt attggctgta
    59221 gcgcctgtac tacggcatgc ccgtggatga tggccaccgt aaataccgaa agtaaaaaat
    59281 cctcgaaatg tgtgttatgc ggtgaatgcg caaacgcctg cccaacaggg gcgttaaaaa
    59341 ttatcgagtg gaaagatatt actgtgtgaa tcttgcaaag gaaaaaatca tgactaacgg
    59401 ttggacaggt aatatattaa gagtcaatct cacgacagga aatattaccc tcgaagattc
    59461 cagtaagttt aaaagttttg tcggtggcat gggcttcggc tacaaaatta tgtatgacga
    59521 agtaccgcca ggcacgaaac ctttcgatga agcgaataaa ttagtctttg ctaccggccc
    59581 attaactgga tctggtgccc cctgtagttc tcgcgtaaat atcacctcac tttctacttt
    59641 taccaaagga aatttagtcg tcgatgccca tatgggtggc ttttttgcag cgcaaatgaa
    59701 attcgctgga tacgacgtca ttattatcga agggaaggcg aaatcaccgg tatggctgaa
    59761 gattaaagat gacaaagtta gcctggaaaa agcagatttc ttatggggaa aagggacgcg
    59821 cgcaacgacg gaagaaattt gtcgcttgac cagtccggaa acctgtgtgg cggctattgg
    59881 tcaggctggg gaaaaccttg ttcctctctc tggcatgttg aatagccgta accacagcgg
    59941 cggtgcggga actggcgcaa taatgggttc gaaaaacctg aaagcgattg cggttgaagg
    60001 gacgaaaggg gtcaacattg ccaatcgtca ggagatgaag cgtctcaatg attacatgat
    60061 gactgaactt atcggtgcga ataacaacca tgtcgtgcca agtacgccgc aatcgtgggc
    60121 agagtattca gatcccaagt cacgctggac agcacgtaaa gggctgtttt ggggcgcggc
    60181 tgaaggcggt ccaattgaaa cgggtgaaat tccgccaggc aatcagaata cggtcggctt
    60241 tcgtacctat aaatccgttt ttgacttagg tccggcagca gagaaataca cagtaaaaat
    60301 gagcggctgc cactcttgcc cgatccgttg tatgacccaa atgaatattc ctcgggtgaa
    60361 agagtttggc gtgcccagca caggtggtaa cacttgtgta gcaaactttg tccataccac
    60421 catcttcccg aacgggccga aagattttga agacgatggt cgtgtgattg gtaacctggt
    60481 tggtctgaat ttgttcgatg actacggcct atggtgtaac tacgggcagt tgcatcgcga
    60541 ctttacttat tgttacagca aaggtgtgtt caagcgtgtt ctgcctgctg aagagtatgc
    60601 agaaattcgc tgggatcaac tggaagcggg agacgttaac ttcattaaag atttttacta
    60661 ccgtctggcg catcgtgtgg gtgagctgag tcacctggct gatggttcat atgccatcgc
    60721 agaatgctgg aatttgggtg aagagtactg gggctacgcg aaaaataaac tctggtcgcc
    60781 gtttggctat ccggttcacc atgccaatga agcgtcagcg caggtcggtt ccattgttaa
    60841 ctgtatgttc aaccgtgact gcatgacgca tacccatatc aactttattg gttccggctt
    60901 gccattgaaa ctgcaacgtg aagtggcgaa agaacttttt ggttctgaag atgcttacga
    60961 tgaaaccaaa aactacacgc caatcaatga cgcaaaaatt aaatatgcta agtggtcgct
    61021 gttgcgggtc tgtttgcata acgccgtcac cctgtgcaac tgggtctggc caatgaccgt
    61081 ttcgccgctg aaaagccgta attaccgggg cgatctggcg cttgaagcca aattcttcaa
    61141 agcgatcact ggcgaagaga tgactcagga aaaattagat ttagctgcag agcgtatctt
    61201 tacattgcat cgtgcctaca cggtgaaact gatgcaaacc aaagatatgc gtaatgaaca
    61261 cgatcttatc tgttcctggg tattcgacaa ggatccgcag atcccggtct ttactgaagg
    61321 tactgacaaa atggatcgtg atgatatgca tgcctcgctc acgatgttct acaaagaaat
    61381 gggctgggac ccacagcttg gttgtccgac ccgcgaaaca ttgcagcgtc tggggctgga
    61441 agatattgcc gccgatctgg cagcacacaa tctactgcct gcgtaaggaa tgggtaagat
    61501 gaaccatcag gatgaattgc cgttagcgaa agtcagcgaa gttgacgagg cgaagcggca
    61561 atggttgcag gggatgcgtc accccgttga tacggtaacc gaaccagaac cggctgaaat
    61621 cctggcagag ttcattcgcc agcattctgt ggctgggcag ctggtggcgc gcgccgtgtt
    61681 tctctcgcca ccctattcag ttgcagaaga ggaactgtca gttttgctgg aaaacatcaa
    61741 gcagaacgga gactacgcgg atatcgcttg tatgacggga agtcaggatg attactatta
    61801 ctcaacgcag gccatgagtg aaaactacgc ggcgatgtct ttgcaagttg tggagcagga
    61861 catttgtcgc gccattgcac acgccgttcg ttttgagtgc cagacgtatc ctcgtcctta
    61921 caaagtcgcc atgctgatgc aagcgccata ttacttccag gaagcgcaga ttgaagccgc
    61981 gatcgccgca atggatgttg cacctgaata tgccgatata cggcaggtag agtcatcgac
    62041 ggcggtgttg tacttgttta gcgagcgatt tatgacctat ggcaaggctt atggcttatg
    62101 cgaatggttc gaggtagagc agtttcagaa cccctgaaat ttcagggaga tgtcatggat
    62161 agggtggaca gttgcagcca ctgcattagg ggataatcag atgtccttca ctcgacgcaa
    62221 atttgttctg gggatgggaa cagtaatctt ttttactggt tctgcgtctt ctctgttagc
    62281 gaacacgagg caagaaaagg aagttcggta tgccatgatt catgacgagt cacggtgcaa
    62341 tggctgtaat atttgcgccc gcgcatgccg taaaactaac cacgcccccg ctcagggaag
    62401 ccgtttatcg atagcacaca ttccagtcac cgataacgac aacgaaacgc agtatcactt
    62461 ttttcgccag tcatgtcagc actgcgaaga tgctccgtgc atcgacgtat gcccgacagg
    62521 ggcgtcgtgg cgtgatgagc aggggatcgt gcgggtagaa aaatcgcagt gcatcggctg
    62581 cagttattgc attggcgctt gtccgtacca ggttcgctac cttaaccctg tcactaaagt
    62641 ggcagataaa tgtgatttct gcgctgagtc ccgactggca aaaggctttc cgcccatttg
    62701 cgtcagcgct tgcccggaac acgcattgat ctttggtcgc gaagatagcc ctgaaattca
    62761 ggcgtggttg caggataata aatactatca atatcagcta cctggtgccg ggaaaccgca
    62821 tctgtatcgc cggttcggtc aacatttgat taaaaaggaa aatgtatgaa cccgtcgcaa
    62881 catgctgaac agtttcagag ccagttagcg aactatgtgc cacagttcac tcccgaattt
    62941 tggccggtgt ggttgatcat tgccggagta ttgctggttg ggatgtggtt ggtgctgggg
    63001 ctgcacgcct tgcttcgtgc tcgtggcgtg aagaaatcag ccaccgatca tggtgagagg
    63061 atttatcttt acagcaaagc ggtcagatta tggcactggt cgaatgcgtt actctttgta
    63121 ttgttgctgg caagtgggct gataaatcac tttgcgctgg tgggcgcaac tgcggttaaa
    63181 agtctggttg cggtgcatga agtttgcgga tttttgttac tggcatgctg gctcggcttt
    63241 gttctgatca atggcgttgg gggtaatggc caccactatc gcattcgtcg tcaggggtgg
    63301 ctggagcggg cggcaaaaca aacgcgattt tatttgtttg gcattatgca gggggaagaa
    63361 catcctttcc cggcaacaac ccagtctaaa tttaatccct tacagcaggt tgcctatgtt
    63421 ggtgtcatgt atggattgct gccgttgtta ctattgacgg ggctgctgtg tctctatccg
    63481 caagccgtgg gagatgtgtt tcctggcgta agatactggt tattgcaggc gcattttgct
    63541 ctggcattta taagcctctt ttttatcttc ggtcatcttt atctttgcac cacggggcgt
    63601 acgccacacg aaacctttaa aagcatggtc gatggctatc accggcacta acgaatgatc
    63661 attacacgag cagacctgcg agaatggcgt atcggagcgg tgatgtatcg ctggttcctg
    63721 cgccatttcc cacgcggcgg tagttatgcc gatattcatc atgcactgat tgaggaagga
    63781 tatacggact gggcggagag tctggttgag tatgcgtgga aaaaatggct ggcggatgaa
    63841 aacttcgcgc atcaggaagt ttcctctatg caaaaactgg ccacagaccc cggagagaga
    63901 gcattttgca gtcagtttgc ccgcagcgac gatcatgctc gcatcggctg ttgtgaggat
    63961 aacgccagaa tcgcaaccgc tgggtatgct gcacaaattg ccagtatggg ttatagcgtg
    64021 cggattggaa gtgtcggctt taacagtcac attggcagct ccggtgagcg cgcacgggtt
    64081 gctgtaaccg gcaattcttc gcggataagc agcgctggag atagcagtcg gattgcgaat
    64141 accggaatgc gagtacgcgt ttgtacattg ggtaaacggt gtcatgtcgc cagtaatggt
    64201 gacctggtgc aaatcgccag ctttggcgca aacgccagaa ttgccaacag cggcgacaat
    64261 gtacacatca ttgccagcgg tgaaaattca acggtagtga gcacgggcgt ggtcgactca
    64321 attattctcg ggccaggggg cagcgccgca ctggcatatc atgacggcga aagagttcgc
    64381 ttcgccgtcg ccattgaagg tgaaaacaat attcgagccg gagtccgcta tcgtcttaat
    64441 gaacagcatc agtttgttga gtgctgatag ctttaccttt aatcaaacga aagacgccga
    64501 cgagcacggg atgcaggctt tacgaccgcc cgagccatcg cctcaccaat ttctgcacat
    64561 gccgtaagtc cctgaacgaa aggctggtcg tgcatcaacc agggcaacgc gccgaacgct
    64621 acgcgcccac gaatatcttc gggttgctgt aacgtataat cttcaccaac atcagggatt
    64681 tcatcccctg ttttctgtaa ttgttcgcgt agtccaggga aaggaatatc tttcacttta
    64741 agcggacgct gcccgcgggc atcaataaaa acgtcaaacg agtagctgtt gtcttccgtt
    64801 ttcaggacgg tgcgcgactc gttaatttcc attttgtagt cttcaccgag agcgagaata
    64861 tgaatgattc ccgcttcacg taaggcaagt aggcgacgaa tagactctga agggattgcc
    64921 gcataattat cgatgaaaac ccgggcaagg cctttactga accgtttatg gtcgtgttca
    64981 ttcagatgtg gaacaatttc ctgtacggct tcatgcaggc gcagaatgac ataacgccag
    65041 ggaacagtat gtttttctcg tttattgcgt tcgacttcct ggagattttt ttctgcccag
    65101 tcaaattggt cgcgttgttt gcgctcggca aaccaggctt gagcaaagga atcgacattc
    65161 aggctctcta aggctatgcg ttgactccag tctggatcag caaacttgat ttcctctact
    65221 atcaatctaa atacccgatc caaaaggcca tattcgcctt tttgaatctc agcatttaat
    65281 gcctgatcag tgacgatgtg taagggctcg tagggaatag ggcaatagaa atcggcttcg
    65341 ggtaaaatac ccgtgcgcga cattaacgta atatttagct tttcacttgc gttatcgcgg
    65401 tgaaaaacga cgtgttgttt atcatcttca atgaacgaac catgctgaat agccactgcc
    65461 attgccgcat ccagtccgct caaggatgtt cccataatac ccacgttaca cgcatcgacc
    65521 tttgcttcca tcaagcctga ccacgggctt ggaaaatacg ttcgggttgc ttcttcttca
    65581 tcaggccaga cgtgacccgt ggcgatcacc gctaaatcaa acgtctcgct gggtaaatcc
    65641 tgatttgtag cgagcatgac gccagcattt gtaatttgca gatcggtaac ctggcatgat
    65701 tcataaacag ccactgcaaa tttttgcttt cgtgcctggt ctactaatcg taaaaattga
    65761 tcgcggaaat attcgcccag cagaattcgc ggtaaaaact gacgatcgtg caaggtttct
    65821 tttttaacgc cataacgctg gagatgactg gcttcttgct tttgtagcca ttcgagatac
    65881 gtacaataaa tcggcggtat ttcaatactg gcaatatttg ccagcatcat ttttgagttt
    65941 tcctcatcac tgtatggcat cccgacaccg gcctcgtcag cctgctcgaa gatagaaata
    66001 gaaagtggag tttgttgctg tagaagcgag aataaggtgt agatccccgt aggcccggca
    66061 cccacaattg caattttttt cattgttact tacctgtacg cattctttcg aatttagtgt
    66121 tatcagataa gtttggttga gatttttaga atttgagaaa tttcagacag ggatgaaaag
    66181 ggcgctaacc gcccttgatt aaattctgtc aggcgagttt tgcctgattg atttgactca
    66241 gtggctcatt gacatcgaaa actttggcga caacttcctc aacgccgagt tttttcagtt
    66301 gggcagtgtg tttttccaga taggcaaggg cgcttttttc atcagtgaaa aggtagatcc
    66361 caccggcttc gtggttcttt tcactttctg tccatacctt ccacagaaaa ccaggttcct
    66421 gattaatcga ctctgcaagt ggtttaagct gctcagccat tgcgtcgcca aatgggccat
    66481 taaaagcaaa atgaagttgt aacaaagttg ccatagcgat tctccttctg ataacgcata
    66541 cgaagacagt gtaaatcttt taacgtattg gatacagaga agaagcagag ggaaaaagga
    66601 gaagaccaga ttaagaaaat ctggtgcgtc cgagtggact cgaaccaccg acccccacca
    66661 tgtcaaggtg gtgctctaac caactgagct acggacgcag gatggtgcgt tcaattggac
    66721 tcgaaccaac gacccccacc atgtcaaggt ggtgctctaa ccaactgagc tatgaacgca
    66781 acgttgtagg tgacaacggg gacgaatatt agcggcagag tgggaaggtg gcaagaggca
    66841 aaacgtaatt ttctgcgcta tttcgaccgt ttgcagagct tttaagcaaa ttggctatat
    66901 tttgttgatt tgcaagggcg atttttattc aggatcgcat ttacatctga tacaacccaa
    66961 taattcgtgt atcagagttt aaacagagtt gtttgccacc atctcaaatc tgtcgtggat
    67021 aaatcctccg tagcctttac acttaggtag caataaaggc acacagatgc agcaaaggaa
    67081 taaatcatgg gatctgatgc gaaaaacttg atgagcgacg ggaatgtgca aattgttaag
    67141 accggcgagg tcattggcgc gacgcaactt actgaaggcg agttgattgt tgaagctggc
    67201 ggaagagccg aaaataccgt ggtcacgggg gctggctggt tgaaagtggc aaccggtggg
    67261 atcgccaaat gcacacagta cggtaacaat ggcacgctat cggtcagcga cggtgccatt
    67321 gccacagata ttgttcagtc cgagggaggc gcaattagtc tctctacgct cgctacggtt
    67381 aatggccgcc atcccgaagg tgaattcagc gttgatcagg gttatgcctg cggtttgttg
    67441 ctggaaaatg gcggtaacct gcgtgtactg gaaggacatc gcgcggaaaa aatcattctc
    67501 gatcaagagg gcggcctgtt ggttaatggg acaacctcag cggtcgtggt agatgaaggt
    67561 ggtgaattgt tggtgtatcc aggtggggaa gccagcaatt gtgagattaa tcagggcggc
    67621 gtttttatgc tggcggggaa agccaatgat acgttgcttg ctgatggcac catgaataat
    67681 ctcggtggtg aagactctga cactattgtt gagaatggag ccatctatcg tctggggacg
    67741 gatggtcttc agctctacag ttccggtaag acgcaaaacc tgtccgttaa tgtgggtggt
    67801 cgggctgaag tgcatgccgg tacgctggaa aatgcggtaa tacaaggtgg aacagtgatc
    67861 ctgttgtcac ccaccagcgc ggacgaaaat tttgtcgtag aggaagatcg cgcaccggtt
    67921 gaactgaccg ggagtgttgc attactggac ggcgcttcaa tgattattgg ctatggcgca
    67981 gatctgcaac aatcaacgat tactgtacag cagggcggtg tattgattct cgacggcagt
    68041 acggtaaaag gtgacagtgt cactttcagt attggtaaca tcaatctgaa tggcggaaaa
    68101 ctgtggctga tcactggtgc ggcaacgcat gtgcagctga aagtgaaacg cctgcgcgga
    68161 gagggagcga tttgcctgca aaccagtgcg aaagaaattt cacctgactt catcaatgtg
    68221 aaaggggaag ttaacgggga tatacgcgtt cagataacag atgccagtcg gcaaactctg
    68281 tgtaacgcac tgaaactaca gccagacgaa gacgggattg gcgcaacgct ccagcctgcg
    68341 taaaaacaca ggcgcacccc gtgaaggttg cgccttgtct ttattagcgg gatgctcgtt
    68401 gcagaatgat ggctgacggc agacgttgca ggtaccgcat acgcagcatc atcataatgg
    68461 ctgccgacgt caggccaata ataaagccta tccagaagcc tgctggcccc ataggttcaa
    68521 cgaccagatc ggtcagtgcc agaatatagc cgcttggcaa gcccagcacc cagtaagccg
    68581 taaaggtgat atagaaaatg gaacgcgtat ctttataacc acgcaaaatc ccactgccaa
    68641 tcacctggat tgagtcagaa atctgatata ccgccgccag caacatcaaa tgcgccgcca
    68701 gcgttacaac ctcgggattg tcgttgtaca acagggcgat ttgctcccgc agtgaaaccg
    68761 tgaatatggc cgtcagggtt gccatacaga cacccaccat aagcccggtc cgcgcagcgg
    68821 tttgcgcatc cagcgttgag ccctgaccca gacgataacc tacgcggata gttaccgctg
    68881 ccgccagcga cattggaagc acgaacatta gtgaactaaa gttcagggca atctggtgtc
    68941 ctgcgacatc aacaataccg agcggagaca ctaacagagc cacgacggca aacagtgtca
    69001 cttcaaagaa tagtgccagc gcaatcggca aaccgagttg aatcagtcgt ttcataaccg
    69061 caggatcggg tttcgcggtg cctttttcgt tacgaatatc gcgcatcgag cgggcgcgtt
    69121 taatgtaaga aaccatggca aggaacatga cccaatacac cgccgcagta gccacgccac
    69181 aaccaacgcc accgagctca ggcataccga aatgaccata aataaagata tagttcaccg
    69241 gaatgttcac cagcaggccg ataaagccca ttaccatacc cggcttggtt tttgccagac
    69301 cttcacattg gttacgggca acctggaaga aaagatagcc cggcgcgccc cacagcaacg
    69361 cacgcagata acccacggct ttgtccgcca gagccggatc gatgttttcc atggagcgga
    69421 taatgtaacc tgcattccac agcaccagca taatgagaac ggaaacaaaa cccgccagcc
    69481 agaagccctg tcgcacctga tgcgcaatgc gctcacgtcg accggaacca tttaattgcg
    69541 cgataaccgg cgttaatgcc agcagcagtc cgtgaccaaa gaggatcgcc ggaagccaga
    69601 tagaagtacc gatagcgacc gccgccatgt cggtggcact atagccgccc gccatcacgg
    69661 tatcgacaaa acccatcgca gtttgggcga tttgcgcgag aatcaccggg attgctaatg
    69721 ctaataacag acgcgcttca ctgatatact tctgcacgtg aacacctttt atttgtagtt
    69781 atatgagaga ctaaaaagcc gcctgaatag gcagcaaaaa gaagaagcag gggaaattca
    69841 gtctattgta gcgaggattt attaattctc cagtgaaaaa atagccattc ttaccccttc
    69901 actggtaagg gcgttttcca cctgctattg tgctgaacag aatgtcttaa ctgatttcag
    69961 gagttgtaag tatgtttacg gggattgtac agggcaccgc aaaactggtg tcgattgacg
    70021 agaaaccaaa ttttcgcaca catgtggtgg agttacccga ccacatgctg gacggtctgg
    70081 aaaccggtgc ctccgtggcg cataacggtt gctgcctgac cgtgacggaa attaacggca
    70141 accatgtcag ttttgacctg atgaaagaaa cgttgcgcat taccaatctt ggcgatttaa
    70201 aagtggggga ttgggtaaac gttgagcgtg cggcgaaatt cagtgatgaa attggcggac
    70261 acttaatgtc aggtcatatt atgaccaccg ctgaagtggc gaaaatatta acctcggaaa
    70321 ataatcgcca gatctggttt aaagtccagg atagtcagtt gatgaaatat atcctgtaca
    70381 aaggatttat tggcatcgac ggtattagcc tgaccgtcgg cgaagtcacg ccaacgcgtt
    70441 tttgcgtcca tttaattccg gaaactctgg aacgcacgac tcttgggaag aaaaaacttg
    70501 gcgcacgcgt caacattgaa atcgatccac aaactcaggc agtggtagat acggtagaac
    70561 gtgtgctggc ggcacgagaa aatgccatga atcaaccagg tacagaagcc tgatctgaaa
    70621 tcgcccccgg agagggggcg atagaatagc ctttagcgag cgactcgaag gccgttttca
    70681 acaccgcgtg agaacaccac ctgccagagc tgaatatcac gggcgcggaa agcacctgcg
    70741 caggcattca gataataggt aaacatccgt ttaaagcgtt cactgtagtt atccgcaatt
    70801 tctggccatg cggcgacgaa tcgttcatac cacgccatca acgtagtatc gtaatcagca
    70861 ccgaagttat gccagtcttc catcacaaag tggggttcgc tggactgagc aatctggcgt
    70921 acagagggca ggcaaccgtt cgggaaaata tatttattaa tccagggatc aacattcaga
    70981 tcggtttttt tcgaaccgat agtatggagc aggaatatgc cttccggttt caaattacga
    71041 tccaccaccg taaaataggt atcgtaattt ttcggtccga cgtgctcgaa catccccaca
    71101 gaaacaatac gatcaaactg gtcgttcagg tcacgataat cttgcagcaa aatggtgaca
    71161 tccaggcctt cacagcgttc ctgagccatt ttttgctgtt cggcagaaat ggtgacgccc
    71221 accacgctta cgtcataatt agatgccatg tagtgtgcca gtccgcccca gccgcagcca
    71281 atatccagta cgcgcatccc aggttttaac tgcaattttt cacaaatcat tttgagcttc
    71341 gcctgctggg cagattccag attatcggca tctttccagt aagcgcagga atattgcatg
    71401 aagggatcaa gcatgcggct gaacaagtca ttacccaaat cgtaatgctc tttgccgact
    71461 atccaggcac gttttttact ctgcagattg aagagacggg cgccggcaat acgcagcgtg
    71521 tctttgaaat gatgggggag ttggttctcg agacctgcgc gtaagacttt gctaaaaaac
    71581 atatccagtc ggtcacattc ccaccagcca tccatataac tttcgcctaa ccccaaagag
    71641 ccttcttgca gaacgcgttt aaaaaaatcg gggtttttca cacgaatatc cgccggggca
    71701 gaaccgttaa tggctatacc ggcacggcta agtaattcgt tggcgatacg gtaccagttg
    71761 tcatccggta cactgacttc ttctatacac gatgaactca tagtttctcc atcactggtc
    71821 ggtgatcaga accttaaaac agcgtagacg ctttttgggc tttgtgagaa atctcgcaga
    71881 aaaaaccgcg agtctataat ccgacgtaga acagagggaa aggaggaacc cttgccgaaa
    71941 tgaccatcca tagtgagtcg ggaacgatcc tgtccccgta aaaacataat atttatcgta
    72001 gctttaacaa ctcgaaatta gtataggccg ctaatttcaa tcatacaaca aaattttgtt
    72061 agcagcctaa gtataagtat atcgatatag atcagtgtga ttcgctatga gcgacttcgg
    72121 cattgccatg attctggcag ccaacttctt cacaacgttg catcatgtaa cccagcgcga
    72181 ccagcactac tgttgataac atcacgctgg tggtggtgag caatggcgtg ctgatactga
    72241 tcagccagga aactaccaga cttgcgagga agcacagacc cagttgcaga gtgttctgca
    72301 acgctgcggc gcgaccagtt gcgtgtggga agggacgcag agcctgggcg acaacaatag
    72361 ggtagatcgc gccattagca atcgccatca cacagaatgg gatcaggatt tcgaccagcg
    72421 acacatggct aatgaagccc gcagcccagg tcgcaatgac gctgacagca aacagcacca
    72481 gcaaccacgg taataactgc ttgccttgcc atttctgcag cgcggcgcga cagccataac
    72541 caccaatcag aaacgcgata gtttgcggga cataacttaa accaataact gccgggctgt
    72601 agcccatttc actaaggatg aacggtgaac cggtcagcca tgcaaaaaaa ctggctgaac
    72661 aggccgcgta tatcagcaca ttgccgcgat aggttttaga acgtagcagg tcggtaaagg
    72721 tcagaccatc ctgactattg ttacgggcct tcgtcgtggg tttgagccag aaaataggca
    72781 gaatcagcac cacggtaatg gcaaaaaggg tggcgaaaat cgcctgccag gaaaaatgga
    72841 ccagcagcca gcttcctaac agaggagcca gggccggaga tagacccacc agcggcatga
    72901 tggtcgcaaa aattcggtta actttctgtg aaggataata atccgtcact aacgcttgcc
    72961 agataaccgc cgcggcgcag acacccacag cctgtacaaa acgcaatacc agcagcatag
    73021 cggcgttttc tacccacagc atccccagac tacctaacgc aaaaattgtc aggccgatta
    73081 ataataccgg tttacgacca taacgatcgg agagcggccc ccacagaagc tgggctgcgg
    73141 caaaaccggc aaggaacaga ctaaggctgg cactgacagc agacgcaggc gtttgcaggt
    73201 cggcctgtat ggcggcgaaa gcaggcagat acatatcggt tgccagaaaa ccgagtacgc
    73261 tcaaacccgc cagccagact aaaaatcttt tcccaggttg catgtatatt ctctaaatct
    73321 ctcaattaaa caccgccgca aagtgtaggg agtgaaatta gtgttgtgaa acggtaatat
    73381 ttgatgactg gtttcaaaaa ttttgcaggc agactatgtg gtcagaatat tcactcgaag
    73441 ttgttgatgc ggtagcgcgt aatggtagtt ttagcgctgc ggcacaggag ctgcatcgcg
    73501 ttccttctgc ggtcagctat accgtgcgtc agctggaaga gtggctggcg gtgccgctgt
    73561 ttgaacgacg gcaccgtgat gtggagctga ccgctgccgg agcgtggttt ctcaaagaag
    73621 ggcgctctgt tgtcaaaaaa atgcagatca cccgccagca atgtcagcag atagcgaacg
    73681 gctggcgcgg tcagttagct atcgcagtgg ataatattgt caggccagaa cgtacacggc
    73741 agatgatcgt tgatttttat cgccattttg atgatgtcga acttcttgtc tttcaggaag
    73801 tgttcaacgg tgtctgggat gcgctttccg acgggcgcgt ggaactggcg attggcgcaa
    73861 cacgcgcgat tccggtaggc ggtcgttatg ccttccggga tatggggatg ctaagctgga
    73921 gttgcgttgt tgccagccat cacccgctgg cgttgatgga tggcccgttc agcgatgata
    73981 cgttgcgcaa ctggccgtcg ttggtgcgcg aagacacctc gcgaacgcta cctaagcgta
    74041 ttacctggtt gctggataac caaaaaagag ttgtcgtgcc tgactgggaa tcatcggcaa
    74101 cctgtatctc ggcaggatta tgcataggga tggtgccaac acatttcgcc aaaccgtggc
    74161 ttaatgaggg gaagtgggta gcattagaac tggaaaaccc ctttccggat tcggcatgtt
    74221 gtctgacatg gcagcaaaat gatatgtcgc ctgcgttaac ctggttgctt gagtatctcg
    74281 gcgatagtga gacgctgaat aaggagtggc tgcgggagcc ggaagagact cccgcaacgg
    74341 gtgattaacg acgatagtcg cggaacgggc cgtcagccac ggagcggcgt tcaatcaagc
    74401 gcggatgcac ttcaatggac tgcggttctt cacgtttgtt gactatacga tccaacaaca
    74461 tgttgaacgc tgtttcaccc aacgaatctt ttggctgatg gattgttgtc agcgccggcg
    74521 taaaatagcg ggcgttgcgc acgttatcat aaccgatcag cgaaacatcc tgcgggacgc
    74581 gcagccccat ttcatcagca gcacaaagtg cgcccattgc catgatatcg ccaccacaga
    74641 agacggcagt agggcgatgc ggctgcgaca ggatttgctg catggcgcga taaccggatt
    74701 caggttcaaa gtcaccctgc acaatccagc tttccggcac cttgatcatt gcttcttcca
    74761 tcgccttcat aaaaccggca aggcggcctg cgccagtgtt acgttccagc gggccgggga
    74821 taacgccgat ttcgcggtga ccgcgttcaa tcagataacg cccggccatg tagccgcctt
    74881 cgaacgcgtt atcaatgacc gcatcggtga agtcagcttt tgcttcaccc cagtccatca
    74941 ccaccattgg gatatggcga tactcttcca gcatcgccag caacggctct gggtactcag
    75001 aacacatcac cagcagacca tcgacgcgtt tttgcgccat catcgacaga taagcccgct
    75061 gtttctcaag attgttccac gcattaccca gaatcagggt gtaacctttc tggaagcaat
    75121 ttttttcaac tgcttcaatg atctcggcaa aataggccgc ttcgctgctg gtcgctagca
    75181 aaccgataga cttggtgtgg ttaaccttca ggctacgcgc caccgcgcta ggggagtagt
    75241 gtaattcttt gatcgctgcc cacacggcgt tgcgcgtttc ttcagcgacg aaacgtgttt
    75301 tgttgatcac gtgtgacaca gttgtagtgg aaacgtttgc tcgtttcgct acatctttta
    75361 ttgttgccat tccatttcac tccagaccct aacttcagct cctgcaaaat cgcaaggtaa
    75421 acgtttgcct ttacacacct tacgcacaac aaaagtgttg cggtacgccg gaaaaacgac
    75481 acggagcgtc aggaaggggt caatggccag tacgcaaata gtgtaagcgt ggaattttgg
    75541 ccgatcttga cgaaaagggg aagtggtaaa aacacttatc attataaatg acgggagatt
    75601 ttttcatcac agtgtgtaaa aatgcgtaat atcacttttt gtgtggtaat aaaaaaggag
    75661 aaaacttgat gagcaccgac cttaaatttt cactggtaac aacgattatc gtcctcggtt
    75721 tgatcgtggc cgtgggtttg actgccgcgc tgcactgatt tcctctcgag ggagcgcgtt
    75781 ctccctcgca tctccttcct gtttgttagc aaaatggcaa aagtgttttc tctttttgtt
    75841 attttcattt ttttgtgatt gctgtcatgc tttcgccact ttaatgaata tcgcgtaatg
    75901 acgcgaagag gtggagataa ggcatgaaaa ttaactatcc gttgctggcg ctggcgattg
    75961 gcgcgtttgg tatcgggaca acggagttct cgccaatggg cttgttgccc gtcattgcgc
    76021 gcggtgtgga tgtctcgatt cccgctgccg gaatgttaat cagtgcctat gcagttggcg
    76081 taatggttgg cgcgccgctg atgacgcttc tactttctca tcgtgcccgc cgcagtgcgt
    76141 tgattttcct gatggcaatt ttcacgctcg gcaacgtact ttccgccatc gcgccggatt
    76201 atatgaccct gatgctttca cgcattttga ccagcctgaa tcacggagca ttttttggtt
    76261 tgggttcagt cgtggccgca agcgtggtgc caaaacataa acaggccagc gcagttgcca
    76321 ctatgtttat ggggttaacc ctggcaaata tcggtggcgt gccggcggcg acctggttgg
    76381 gtgaaaccat cggctggcgg atgtcatttc tggcaacggc ggggctggga gtgatttcaa
    76441 tggtaagtct gttcttctca ttacctaaag gtggtgcagg ggcacgacct gaagtgaaaa
    76501 aagagctggc ggtattaatg cgtccgcagg tgctgtctgc attgctgacg acggtactgg
    76561 gagctggtgc aatgtttact ctctacacct atatctctcc ggtactgcaa agtattaccc
    76621 acgcaacacc ggtgttcgtc acggcaatgc tggtgctgat tggtgtcgga ttctctatcg
    76681 gtaactatct cggcggcaaa ctggcagatc gttcagttaa cggcacgttg aaaggctttt
    76741 tgttgctgct gatggtgatt atgctggcaa tcccgttcct ggcccgcaat aagttcggcg
    76801 cagctattag catggcggtg tggggcgctg caacctttgc ggtcgtaccg ccgttacaga
    76861 tgcgcgtgat gcgtgtcgcc agtgaagcgc caggtctgtc ttcatcagtc aatattggtg
    76921 cctttaatct tggaaatgcg ctgggagcag ctgctggtgg tgcggtaatt tccgctgggc
    76981 tgggatacag ctttgtgccg gtgatggggg cgattgtcgc gggactggca ttattgctgg
    77041 tgtttatgtc agccagaaaa caacctgaaa cagtttgcgt tgctaacagc taattaaaca
    77101 aaaacgcaga aggataacct tctgcgttac agttgctgac aacgtgcgat ttatcttgcc
    77161 gaatgcgctg ttaacgcctc atccgcgttg gcatggtaag cgaagcgcat caggcaatac
    77221 tgcatttgcc atcagttatt atgcagcgag atttttcgct acgaattccc agttcaccag
    77281 cgcccagaag tgctcaagat agccaggacg tgcattgcga tagtcgatgt aatatgcgtg
    77341 ttcccagaca tcaacggtta gcagcggagt cgcatcggtg gtcagcggag tacccgcgtt
    77401 agaggttgaa acgatagcca gtttgccatc gctgtttttc accagccagg tccagccaga
    77461 accaaagttt ttgatcgctg catcagtaaa ctgagctttg aaatcggcaa agctgccaaa
    77521 agatgcggcg atagcttcag cgacttttcc agtcggttcg ccaccggcgt tcggtgccag
    77581 gcagttccag tagaaagtat ggttccagac ctgagctgcg ttgttgaata cgccaccttc
    77641 agagctgcga ataatctctt ccagtgattt accttcaaac gcggtacctt taatcaggtt
    77701 gttcaggtta gtgacataag tctgatgatg cttgccgtag tgatactcga tagtttccgc
    77761 agaaatgtgc ggtgccagag catctttagc atatggtagt gcaggtaatt cgaatgacat
    77821 tgctactctc ctttattatt aatttgcata cgtacaatag ccttattgtg cgtatggata
    77881 gggtagcaaa ttaaaaggtg agacaaaaga tgaacttacc ctgttgccgt aacaacaggg
    77941 taaagtttta gcgaagtgtt tttggggtca ttacccggcg agcgccaaca tagtggcgct
    78001 gccagtagtc ttcactgaga gaagtgattt gaatttcctg acctgtgcgc ggtgactgaa
    78061 taaatttgcc gttgccgaca tacacgccga catgatcggc tgtaccgcgt ccctgagtac
    78121 ggaaaaagac caggtcgccg tttttcagtt cactacgttc gattggcgct gcatcgcgca
    78181 ggtgatacat ttcattcgcc gtacgcggaa tacgaatttt caccaaatct ttataagcgt
    78241 aataaaccag gccgctgcaa tcaaaaccgg tacgcggtga gctgccaccc caacgatatg
    78301 gcttaccaat ttgctgcatc agtttattca ttgccacttt tgtggctttt tgcaccttcg
    78361 ctttgtgtgc gtcggcaagg gttcctgacg ctgcattttt gactttcaca caatgcgatt
    78421 tatagccctt acgtttggta cacttttcgg taaagctggc tgaggccgtt ttattgacgg
    78481 tatgtttgct gcgagttttg gcgtttttag aagcggtaat ggaagatttt ttaacggaag
    78541 aagcggtttt gcttttggtc gtacttttac tggcggcttt tttcgctgtt ttctgggtct
    78601 ttttggtggt tgccgtgctt ttctttttat ctgctttttt ggtgatatga gtggtagcag
    78661 aactctccct ggcttgcttt gaagcatggg ccataggcgt taaaggcagg gtggtaaaaa
    78721 gtaaagcaca gagcgtgatc gaaatacggt ttatccgcgc cactgagcgt ccctctgtta
    78781 aggcaggtct atcatgcctg cgtctgattt acaaaacttg atgattctaa tgcatagaag
    78841 aagagaaagt taatagcttt tattgttaaa aaatacgttt cgttggcgag cgcaaaaaaa
    78901 tgcagcagtg aaagctaaaa atggtcataa atacgccagt ttgccgtgca tcagggaaga
    78961 aacgaatatt tgaaatgcaa caaatagagc aaagggataa aatagcaaaa gcgtgatgaa
    79021 aatgttattt tccgatgccg cactgacccg ctacaatgtc agattactgc cgtaaagaag
    79081 tttgaggaag caagacaatg agcaccacta tcgaaaaaat ccaacgccag attgctgaaa
    79141 acccgatcct gctgtacatg aaaggttcac cgaaactgcc gagctgcggt ttctctgccc
    79201 aggcagtcca ggcgcttgcc gcatgtggcg aacgttttgc ctatgttgat attctgcaga
    79261 atccggacat tcgtgcggag ctgccgaaat atgctaactg gccgaccttc ccgcaactgt
    79321 gggttgacgg cgagctggta ggcggttgtg atatcgtgat cgaaatgtat cagcgtggcg
    79381 aactgcagca gctgatcaaa gaaactgccg ctaaatacaa gtctgaagag ccggacgcgg
    79441 aataatccgt cctgcaatac tgacggatgc gaacgggcat ccgtcagtcc tttctctatc
    79501 cccaatccag cccttgtggc gaacttgaaa atcccgcttc acgcagcagc gtaaacatcg
    79561 gcgtttgcca gaccggtaga tcattcactt ctgttagcgt aaagcgcagc cgtggttcgc
    79621 gacgcagtgc ggtagtcagc gcgtggaaaa cctctggggc gagtaattcc tctttttcct
    79681 gccagaccag catttttttg ccaccttgcg ccagatagag taacaatttg ccgccagaaa
    79741 cgaccaccag cgcacccgcc cgacgcgttg gtaccagcgt tgccggatgt gcaggccagg
    79801 gcagaagatt tccccacaca ttagccggat cgttggcgga gagcgccact ggtgtatagt
    79861 ggcgcgtttg cgcggcttgt gtcgccaggt cgcgcaagcg gtcaatagtc agacgttcag
    79921 cgaattgcgc gccacccaga ccttctacaa aacgacctcg cataattcgc ccggaatctt
    79981 ccatacttcg acaaagcgtt tgcatcgatg gaaacccgcc agggatattt tcggctatca
    80041 ccgcctgacg actgatgatg ccgtagcggt cgagcatatt ttccgccagc gccagcatcc
    80101 tttcggtatc gtttagtggc tccacctgca ataacgacca gcgtccagcc agatttggtg
    80161 tgttgtaaga tacccgcggc gagacgggtt gtgcatagac aggacgccca cgacgagccc
    80221 ggtgactgcg gcgagttgag gtgcgtgcgt tggaactgct gcgggtgagg gcgcgtaacg
    80281 gtgcccaaat atcgctggtg atgacgcctt gccagaccag cgcccataac gcctcttgca
    80341 gtgccgaggg atcaaccgat tcgccgattt tgtcgcgtat ccgttggctg atttgttgtg
    80401 caaaccaggc tcctccgtca gccagaacag cgactatcgc ttgttgcagc gccgaacgat
    80461 tcgcctgatc cgcttcggcg ggagtgaacg attctgcagc atattcctgt agatgcagtg
    80521 ccaccaggcc gtcatcttca cccagttttt tttgccccga ccagataacc gcaccggttg
    80581 ccagtaattc atcgagcact tctggcgaat agtcgtgtac gcgggcaggc aagatctggc
    80641 tttcccagag tgaggcgggt aagccgactc cggcaagctg ttcgatcacc cgcatcacgc
    80701 catccacccc ctcataaacg cctggcgatg ttgaggcaaa gagcgccggg ctaccatcgg
    80761 tggcgggtaa tacgccctga cgttccagca gcaatcgcgc ataggtcgtg gctgcaacgg
    80821 gacgcgtcgc ttctctggcg gcttgcagcg agcgcaaacg cagacgacga aatacttcat
    80881 cgctcaccca gatgtcttgt tgcagattca tcaccagacc ctgttcgcgc agttgctgaa
    80941 gctgctcttc gacaatggca ataccgagac taaactcatg agccagttgt tcagccgtga
    81001 ccagagcatg agcccggaga tagcgtagaa agaggtcgcg aagcgggtaa ctgactctat
    81061 gcaaataaat ctctggcagt gactctggta atcgtacgcc gagggcatcg cgcagcctgg
    81121 cggcatcatc catacaggca agacgctcct gtccactaat catcgtcggg aagatgcgtt
    81181 ttactgtaag aagattttcc agatacgagg caatctcttc actgctgcct gtatgccgtt
    81241 gcgccaggtc ttcaacggtc attggcccca gttcgcgcag caggtcgaac aatccttctt
    81301 cacctttcgc tcttctgcca ggagccagtc gttgcaactc ttcttccacc tggcgaatga
    81361 cctgcgggtc gagtaattcc cccggatcga cctgtccgag tagattgcgc agtaactcgc
    81421 tgtccagcga cagtacggat gcccggcgtt ctgccagcgg ggcgtcgctc tggtacataa
    81481 attccgcgac atagccgaac aataaacttg tggcgaaagg cgagggcgta gtagtcgtta
    81541 catcggatat ttgaatttcg cctccgttca ggcgacgcat caaacgttcc agtgcgggaa
    81601 gatcataaac atcttgcaga cattcgcgta gggtttcgag aatggccgga aaatccggat
    81661 atccctgagc gatttccagc aactgactgg cgcgcagccg ttgttgccaa agcggggtgc
    81721 gatggcccgg cgtgcggccc ggcattaata atgcccgcgc ggcgcattcg cgaaaacggg
    81781 cggcgaaaag tgccgaactg cctaccgcct cgcggacaat ttgcagcaac ttttctggtt
    81841 caaacaaaaa aatcgcggcg tcgggcaatt taccatcagt gtcaggaatg cgcgcaacaa
    81901 tgccgtcatc actggcgacc acagacgcgt cagcgcccca aagcgcatgt attcgcccgg
    81961 caatcgccag cgcccagggt tcatgcaccc gtcttccata gggagagtgc aaaataatac
    82021 gccagtcgcc aatttcatca cggcaccgtt cgaggaccag atgacgactc cccggaacaa
    82081 tacccgtcgc gttgcgctgc tcgtcaatca acccctgaat attggcgatc gtattttctt
    82141 cagccagcca cgggggaata gtcccggaaa agaacgctcc atccgccagc aaatgaagaa
    82201 aatcgccgat catctcgcct aattcagccg gacgtccgtt accttcacca cgccagaagg
    82261 ggagccgggc agaacgaccc ggagcaggag tcacaatcac ctgatcgcgg gtgatttgct
    82321 ggatccgcca tgaggttgcg ccgagagtga taatatcgtt cacccgcgac tcatatacca
    82381 tctcctcatc cagttcaccc acccgtcgcg aaccggcctg ttcttcccct tcgggtaata
    82441 acacgctata catgccacga tccggaatgg tgccgccgct ggtaaccgcc aacagctgag
    82501 cgccaggtcg ggcggtcaat atcccggtct ccctgttcca gaccagtttg gggcgaaaag
    82561 cagaaaaatc gccagaggga tagcgtccgg aaagcatatc cagcgtggcg tcaaaaacac
    82621 gtcttggcag atctttccac ggtgcggcac ggcgtacgcg ggagtaccat tcgtctacct
    82681 gtaatgcatc catcgccgcg gcggcaacgg tgtgctgcgc aaggacgtcg agtggattat
    82741 gcggaggtgt caggttttcc agcctgcctg cgaacataca ctctacaatg actgcggaat
    82801 cgactaagtc acgcctggtt cgggggaaaa aaagcccttt ggagatacct cctatctggt
    82861 gtccggcacg tccaatgcgt tgtaacccac tggcaacaga aagcggcgtt gccacctgaa
    82921 tcaccagctc cacagcgccc atatcaatcc ccagttcgag actggaagtt gcgaccacgc
    82981 agcgtaactc acccgatttc agcgcctgct cggtgattgc tcgttgttct ttagagacgg
    83041 agccgtggtg tgagcgggca ataaaaacgt cgctactttg tacacggtta gaggttgcgc
    83101 cagaggtcga ctcgaaatgg gccgcatcag cggcgataga cggggaacgc tgtaagcgtg
    83161 cggcgtaaag ctcatttaat cgtgccgtca gtttttccgc cagcccccgc gaattagtaa
    83221 agacaatggt cgagcgatgg cgcaacactt catcaaggat acccgtttca atatatggcc
    83281 agatggagcc ttcccggccg gcatggctgt cttcgccagt gccgctggcg accgatgaga
    83341 catcatccat attggcgact ggtacgacaa tccgtatctg cggatgacgc attgcgggcg
    83401 ggttgactac cgtaaccggg cgatcgccac caagaaatgc tgccacatcg ctggctgagc
    83461 gcacagtggc agaaaggcca attcgctgtg ctgaggtgtg gagcagcgca tcgagccgct
    83521 ccagacttaa cgccagatgc gcaccacgtt tacttcccgc taccgcgtgg acttcatcaa
    83581 taattaccgt ttcgacgccg cgtagcgttt cgcgcgcgcg ggaggtcagc atcagataga
    83641 gtgattcggg tgtggtgatc agaatatccg gcggattacg ggtgagtttg ctgcgttcct
    83701 gtgcaggcgt atcgccagta cggatcccca cgcgaagatt gacttccgtt tcgccgcgcc
    83761 gccgccgttc atcggcaata cccttcaacg ggatctgcaa gttgcgctga acgtcggtgc
    83821 ccagggcttt tatcggtgaa atatagagga tgcgtgaggt tttacgttta tgcgcctcgc
    83881 gggtatcttc gccgccttcg cggaagagtc gatcgagggc gtagagaaat gctgccagcg
    83941 ttttcccgga gccagtcggt gcaatcacca gcgcatgttc gcctcgcgcc gccacatgcc
    84001 aggtttgcga ctggacagcg gtcggctgtt taaaggcgcg aagaaaccag tcgcgggtcg
    84061 ccggtgaaaa cacgtccggc aggggcgatg aagggtttgg attatctgcc attaacgcta
    84121 ttccccgtga atatcatgca tgatgcgtgg aatagcctca ctatagatgc ctgaatcatt
    84181 gcatgtcatc aggcatcgac tcgattacac ctcttcggcg gcaggtagcg gccagcctcc
    84241 cagacgtttc cagcggttga cgatttcaca aaacagcacc gcagtgcgtt cggtgtcgta
    84301 cagcgcggag tgcgcctggg tgctgtcgaa gtccatgcca gcggtctggc aagcctttga
    84361 caatacggtt tgtccgagtg ccagcccggc cagtgcagca gtgtcaaaag tggcgaaagg
    84421 gtggaacggg ttacgtttca gtgaggcgcg ttctgcggcg gccatcataa agctgtgatc
    84481 gaaattggcg ttgtgcgcca ccataatggc gcggttacag ccgctcgctt taataccttt
    84541 acgtacaact ttaaaaattt cgtgcagcgc ctcgtattcg ctgaccgcgc cgcgatcggg
    84601 atcgttcggg tcaatgccgt tgaaagcaag ggcttctggt tgcaaatttg cgccgacaaa
    84661 tggttcgacg tggaaatgta atgtggtgtc cggcatcagc cagccttgtt catccatttt
    84721 cagggtgatg gcggcaatct caagcagcgc atcggttttg gcgttaaatc cagctgtttc
    84781 aacatcgatc acaacaggat aaaagccacg aaaacggtcg cacagaccgg taagttgagc
    84841 gttatcggac atctgattct cttaatacgg ataaattgca gcgcgcatta tgacaaatat
    84901 tacttgtcga tgcagtaaag atgcgggcgc gatgagttcg cgcccggcag gagattagtt
    84961 gcccagaccg cgaccggcgt ctttctcttc gattaactca attttgtaac cgtccggatc
    85021 ttccacaaac gcgataaccg tagtaccgcc ttttaccgga cccgcttcac gggtcacgtt
    85081 acccccgttt tgacggattt tttcgcacgc ttcagcggca ttatctacgc taagcgcgat
    85141 gtgaccataa gcagtgccga gttcgtattt atccacgccc cagttgtagg tcagttcaat
    85201 caccgcttct tcggtttccg ggccgtagcc aacaaacgcc agtgagtatt tgtattccgg
    85261 gttttcgctg gtacgcagca gtttcatgcc cagcactttg gtataaaaat cgatggagcg
    85321 ttgcagatcg ccaacgcgca gcatggtatg aagaagacgc atctttttat cctcaaaatg
    85381 gctaacagat tcaaaatgtt gttttagtat agcggcggct ttacgccgct cgcaatgttg
    85441 gattacaacg tcgggtaatc agtgtagcct tccgcaccgc caccatagaa actttcagca
    85501 cgctgtgggt taagctcagc tttgcgctgc aagcgggcga ccagatccgg gttcgcaatc
    85561 cagtcacgac caaatgccac cgcatcaatt aaccctttgc cgatcagcgt ttcagctttt
    85621 tctactgtgt atgcacctgc gccgataatc ggaccgtgga aacgggcgcg tactttttcg
    85681 cggaacgcat cagtatacgg ttcacccccc gcccaatctg gttctgacat atgcagataa
    85741 gcaatgccgc gtttacccag ttgttcaatc agatacagtg catcggcttc ttcattcggg
    85801 ccgttatccg tgttctggaa agtaccgatt ggcgaaacgc gaatgccaat gcgatcggca
    85861 ccccattctt caatcccggc atcgaccact tccagtacca aacgtgcgcg attttccacg
    85921 ctgccgccgt actgatcggt acgatggttt gaagaaggag aaaggaactg atgcagcaaa
    85981 taaccatgag cagagtggag ctctaccaga tcaaaaccgg cttcacgcgc gttagcaata
    86041 gcctgacgga aatcattgac gatacttgga atctcttcca gttcaagcgc acgcggcatg
    86101 gatgtttcaa cacggatcgc ctgaccattt tcatcgcgca gagaagtacg tgttcccgcg
    86161 ctaagtgctg aaggcgctac tggtgcctgg ccgccaggtt gcaggctggc gtgagaaatg
    86221 cgtccggtgt gccacagctg tacggccata tgcccatttt cagcatgaac gccagcggta
    86281 atttttttcc atgcggcaat ttgttccgga ctatggatgc caggtgcacc agcataccct
    86341 tttgcctggg cagaaatttg cgtggcttcg ctaataatca aaccggcact ggcacgttgg
    86401 cgatagtatt ccgccatcaa cggggtaggg atgtcccccg gttcaatact gcgcagacgc
    86461 gtcagcggtg ccataaaaat acggtttgcc gcagttattg cgcccacttt cagtggggaa
    86521 tacagttttt cagatgacat aaaggctccc ggtcgtctgg tgataaaaaa tgcctgctaa
    86581 acggcaggcg tcgcaataat ggtttttaca tgtgccagcg cgttttccag tggctcggaa
    86641 ttgcgcgaaa ttttggcctg cagattcgca cccagccata gtgcgtaaag cacttgtgcc
    86701 tgttgcagcg gttcgccaca aaaggttaaa caatggctat cacggccatt ttccagagcc
    86761 tgcgagagca gggcgatcac gccgcgagcg cctttatcca tcgcgctacg catatcttct
    86821 gacagatcgc acacttcggc agagagtttt actgtcaggc aaccactgat ggttccatgt
    86881 tggcaaaact ggttcagtgt ttgctggtaa taagccagta tgcggtcgcg gtagttacct
    86941 tcgccggatt gcagcaactc agtcagtcgt tggtgatatg tggcgtaatg gcgctcaagc
    87001 atggcaacgc caaacgcttc tttagagcga aagtagtgat agaaggaccc tttcggcact
    87061 tcagcggttt ttagtaattc gcttagcccc atcccggtga atccacgttg caggcaaagt
    87121 tgctcgcccg tcgccaggag atgttcgcga gtatcatgtt cggtgtgttt gttcatgcgt
    87181 tggagtgtag tagaccagtc ggtctagtgc aagcaaagtt gctactttgg gcattaacag
    87241 cttcaataat ggtaataatc ttatttgagg agggcgaagt ggcggagcaa ttagagttct
    87301 ttcccgtcca gagcccgtgc cggggaattt gccagtctga cgaacgcggg ttttgtcgcg
    87361 gctgttttcg aagccgggat gagcgtttta actggaataa aatgagcgat ggcgaaaaac
    87421 aagaggtgct gcgcctttgt cggcagcgat taatgcgcaa attacgtgca aataagccag
    87481 cgtcatcaga cgaaccggag caaccatcac tcttttgagc ctaatcttgc gtatactcct
    87541 ctcatatctg ataagaggaa gcgattatgg ttcagcgtat tactattgcg ccgcaaggcc
    87601 cggagttttc ccgttttgtg atgggctact ggcgattgat ggactggaac atgtccgccc
    87661 gccagctggt cagttttatt gaagagcatc tggatctcgg cgtgaccacc gtggaccatg
    87721 ctgatattta tggtggctat cagtgcgaag cggcgtttgg cgaggcgctg aaactggcac
    87781 ctcacctgcg tgagcggatg gaaatcgtca gtaaatgcgg tatcgcgacg accgcgcgtg
    87841 aagaaaacgt cattggtcat tacatcactg accgcgatca catcattaag agcgccgaac
    87901 agtcgctaat taatcttgcg accgatcatc tggatctgct gttaatccac cgcccggacc
    87961 cgttaatgga tgccgatgaa gtggcggacg cgttcaaaca tctgcatcag agcggcaaag
    88021 tgcgtcattt tggcgtatcc aacttcacgc ctgcgcaatt tgccctgttg caatcacgtc
    88081 tgccgtttac ccttgccact aatcaggtgg aaatatcccc ggtacatcag ccgttactgc
    88141 tggatggcac gctcgaccaa ctgcaacaac tgcgtgttcg tccgatggcg tggtcctgcc
    88201 ttggtggtgg tcgtctgttt aatgatgatt atttccagcc gctgcgtgat gaactggctg
    88261 tggtggcgga ggagttaaac gcgggctcga ttgaacaggt ggtttacgcc tgggtattac
    88321 gtttaccgtc gcagccgctg ccaattatcg gctgaggtaa aattgagcgc gtacgggcag
    88381 ctgtcgaagc agaaacactg aaaatgaccc gtcaacaatg gtttcgtatc cgtaaagcgg
    88441 cactggggta cgacgtaccg taagcacttt taggaatagc cgccgttcaa aaatgtgtca
    88501 ctggtttaca cttattcagg aatgcacaat gaacggaggt cctatgaaac gttttagtct
    88561 ggccattctg gcgctggttg ttgcgaccgg cgcacaagct gccagtgaaa aagtcgagat
    88621 gaacctcgtc acgtcgcaag gggttgggca gtcaattggt agcgtcacca ttactgaaac
    88681 cgataaaggt ctggagtttt cgcccgatct gaaagcatta cctcccggtg aacatggctt
    88741 ccatattcat gccaaaggaa gctgccagcc agccaccaaa gatggcaaag ccagcgccgc
    88801 ggaatccgca ggcgggcatc ttgatccaca aaataccggt aaacatgaag ggccagaagg
    88861 tgccgggcat ttaggcgatc tgcctgcact ggtcgtcaat aatgacggca aagctaccga
    88921 tgccgtcatc gcgcctcgtc tgaaatcact ggatgaaatc aaagacaaag cgctgatggt
    88981 ccacgttggc ggcgataata tgtccgatca acctaaaccg ctgggcggtg gcggtgaacg
    89041 ctatgcctgt ggtgtaatta agtaattaag aggccagcgt accttgcggt ggtgcttgct
    89101 caagttgcga aagcgagcag tacagccgcc agacaattgc cgccagctca cgggcggcag
    89161 gttgatgatg gcgggcaagg ctgtcgcaaa tccgctgtaa ttcttcaagt gtggccgcca
    89221 gtgatttttg ctgaacgcca cgctcactca tcactccccg caacagtgaa atacagttat
    89281 cccgtactcg cgataacgga tcggaacgcg attcccagtc gcgtaattgc cagacaacat
    89341 gagaacagtt cagcagcact ataccccagc gtaataacca acggcgagcc agcgcatcct
    89401 ggctgttact caactgactg acgtgatgat aagtgagcga ttcaaattcg ctttcactca
    89461 gtgttggatg gcggcttagt tgatcgacaa aatcccggcg cagcgcgcga atatggcggc
    89521 ggcttttgcg agcatccgat cctggacgca gaatggcgaa cgctaaccac gccaacgcga
    89581 cgccaacgat ttttgccaga ttatcgttaa gaaaatcagc aaaattatac accgggggat
    89641 tagtgacggc gataaaagaa cccataaaaa caatcagttg cccccacaat gcggcaaatt
    89701 tgggcatctg caatttaaga agctgcattg tcgccagcag tggaaagaga aacagtaaaa
    89761 attgccacag atcgctaatc tggaccatca gaccaaattt gaccacaaag ctgaatagcg
    89821 aaagtaacac cagcgtgcgc atcagcagtg acaacgactt aaacggtgct gcgacggcgg
    89881 agtagagtac gcagctaatc gctgccagcg ttaatgcatt ggcaccggca tcccattgcg
    89941 aagcaatact ccatgcgcca atcatcatca acgtacaaaa tgtacgcagt ccgctccaca
    90001 tagcttcggc gttatcggta tgacgagcca ggccggatgt ccgtgggagt ctggcatgat
    90061 catctacatc gctttgcaga cgatgtaatt cctgactact ttgcagataa aggcggcaaa
    90121 aataacgtag tcgctgccag aaggcgacgt gccgatagtc ggcgacgttg gtcgggcgta
    90181 gcggggcgat aatacgtgcg acggtgtaaa catctgtttg cgaactggcg agcgccgtca
    90241 gcaactgttc gagaatttct cgcgtggcac ctggcggtga gggccagttg agcaacatac
    90301 gtcgcaggct ggagatgaca ctggtcatac gtaattgctg gtggagcagc gcattaaggc
    90361 gcgcgttttg ctggcgaaaa cgatagtggc tccagaaagc ctggatacgc agcaaattca
    90421 tggtcagtat ctgcccaatc accccttcat gtgctgcacg aatggcatcg gttgtttcag
    90481 gctgccagag taaactggca tgttccagta atcgggcgtg catgtttttc aatgcggtta
    90541 aaagggctgt agcatcggaa ctgctaggca ggatcatcat catcatgccg ccgcacaaaa
    90601 taccgacaat cacctcgcaa acgcgcgcct gagcgatatc ccacagctgg ctggcctcag
    90661 taatattaac catcggaaag gcgatgatgg cagccgtgta gcccgccagt tgaaatgcat
    90721 acgcgacgtt attcgtgaag tgcgcacagg cccaggtaca aaagccaagc cacgccgaca
    90781 tgctcaatag aaaaaaccac ggctcattga gcgtatgccc ggcaagaagc agtgccgcaa
    90841 tggctcccag taaactgcca gcgatgcgtc cgaggctttt gctgatgaca ccgccaacgg
    90901 tgggaaagct aaccactgca gccgaggtca tcgcccaata gggttcatcc agattcaaat
    90961 aataggcaac cgtcagcgcc agacacatgg caatggtatt gcgtaacgca taacgccatt
    91021 gcgccagcgt ggccctgaac cagggcaaat tgcgcaagga ccatgacgat gcgttcatta
    91081 ttgacctacg gcaatgctac aggtggtgcc cgacaccagc gtgacatcgc ctggaacttt
    91141 atccagcgca aagcggacgg gaacgcgttg ggcgagacga acccagggga cgttaggttt
    91201 cacatccgga ataaggcttg agtcactttc aacgctctga tcataaatcg cccgaccgat
    91261 actggaaacg tgaccctgta acgttttgtt atcactatag agggtaattt gtgctggtgc
    91321 accttcgcgg atatggcgca acttggtttc ttcaaaataa ccaatgacgt aaaacgagtg
    91381 gctgtcgaca agggcaaaca gcggtttccc ggtgtcagcg taatcgccga tgcgggtagt
    91441 gagattagtc acccatccac tcaccggggc gcgaatttcc gtttgcgcca gttgccattg
    91501 tgtctgtttc agcgtcgcct gtgcggcatc aacactggcc tgcatcgctt taacattgag
    91561 gttagcggta tcgagttctt cggcagaaat aaaattttgt gagagatgac gacgacgatt
    91621 cgcttcgtta ttggcttttg cgaggtcaga ttgcgctttc gccagctgag cctgtgcatt
    91681 cagctcggcg atctgaaagg gcgttttatc gatcgtgagc agaagatccc ccgcgttaac
    91741 cagctggttg tcttttatat tcagctcaac aatacgtccc gacacctgtg gggtgataga
    91801 aacctgttct gcgcgtattt tcccgtcgcg tgtccacgga gattgcatgt aataattcca
    91861 cagccaccat ccggcaagaa ccgcaactac cgctacaatg attgttgaga aatacttaat
    91921 tgttttaatt gacatagtta ccacgcaatc agtattgcca gagcaaggca aacgcaaatc
    91981 gcaaacagcg ataaatccat taacaaggga tgccagatgt caccggcgta gatccagcca
    92041 cgaagcaggc ggtgtacgac aagccagatg acgaatccaa acgcgaaggc tttgaaaaac
    92101 ggaggaaagt agacggacgc accgaacacc agatcttgta agggcaatcc tgttgcattg
    92161 agcataaact tcacaaagta gattccttta cgaccgaata tattgcgtta gattaataaa
    92221 tattctttaa ctgcgaaaaa tttacgcgca atttctgaaa aatatgtctg acggtaacca
    92281 aatgcagcaa tacatttgtt ttagcaatac aattgctgca cactattcta aaagccgcat
    92341 aatatcttag caagctaatt ataaggagat gaaattggaa tcgccactag gttctgatct
    92401 ggcacggttg gtgcgcatat ggcgtgctct gatagaccat cgcctgaaac cgctggagtt
    92461 aacacaaacc cattgggtta cgttacacaa tatccatcag ttacctccag accagtcgca
    92521 aattcaactg gcaaaagcga ttggcatcga gcagccatca ctggtccgta ctctggacca
    92581 actggaagaa aaagggttaa tttcgcgtca aacttgtgcc agcgatcgtc gggctaaacg
    92641 tattaaactg acggaaaagg cagagccgct gatcagcgaa atggaagctg ttattaacaa
    92701 aacccgcgcg gaaattttac atggcatctc cgcagaggaa ctggaacaac tgattacgct
    92761 catcgcaaaa cttgagcata atatcattga gttacaggcc aaagggtgaa atgaaggggg
    92821 cgtgtggtca gtaacctgac cacacgcaaa cttatttagc gcggagaaac ggtcacctga
    92881 ctgccattgc tggccagtac gacacgttgg cccggagaga aacgagtgtt gccttgtttc
    92941 tgtaccacca tgatggtatt accatcgtct ttacgaattt ccagctcgac accttgcgtt
    93001 ttgttcattg cactctgtac gccctgacca gctacgccac ctgcaacagc gcctgctgca
    93061 gtagccagag aacgcccggt tccgccacca acagtatttc ccaggaaacc accaagaaca
    93121 gcaccgccaa ttgcaccgat aacgttggaa tcatcaccgc cctgaatctg taccggacgt
    93181 acgttaacga tggttccata gctgacattc tgtacttgtt tcgcttcaga agcggtataa
    93241 acatcccctg acagggtgtc gttattaaca caaccgacaa gagacagacc taccattgaa
    93301 acaaccaata cgcgtttaat cattgaaaaa tctcctgttc accatgaaac gccacgcaag
    93361 catccctcat ggtcaaagta tatggcatat gtgaatccat ggttacatgt tctaccaaat
    93421 atgagcaaat cataatgaaa gtaggatcaa ccaattataa acaaaacatt catgatgatt
    93481 attgatttga agataatgtt ttactaccaa cggtaaaaat cggtcattcc gcatggtatt
    93541 cacgaaaagt ttatgtttga gtgtgcgcaa acgtcagatt gaaaaaggat gaactatgaa
    93601 atcgggccgc tttattggcg ttatgtcagg caccagcctt gatggtgttg atgttgtgtt
    93661 ggcgacaatt gatgaacacc gggtcgcaca gctggcaagt ttgagctggc cgatcccggt
    93721 gtctctgaaa caggctgtac tggatatttg ccagggccag cagcttacac tttcgcagtt
    93781 tggacagctt gatactcaac tcgggcgact ttttgctgat gcggtcaagg ccttgcttaa
    93841 ggaacaaaac ctgcaggcga gagatatagt tgcgatcggc tgtcacggtc aaaccgtctg
    93901 gcatgaaccg acgggcgtgg caccacacac tttacagatt ggcgataaca atcaaattgt
    93961 ggcacgcacc ggaattacgg ttgtcggtga ttttcgccgt cgcgatattg ccttgggagg
    94021 acaaggcgca ccgctggtac ctgcgttcca tcatgccttg ctggctcacc caaccgagcg
    94081 acgaatggtg ctcaatattg gcggcatcgc caatctgtca ctgctcattc ctgggcagcc
    94141 ggttggcggc tacgataccg gtcctggtaa catgctgatg gatgcctgga tctggcgtca
    94201 ggccggtaaa ccttacgata aagatgccga gtgggcacgg gcgggtaaag ttattctccc
    94261 actgctgcaa aatatgctca gcgacccata tttctcgcaa cctgcaccga aaagcaccgg
    94321 acgcgaatac tttaactacg gctggctgga gcgccattta cgccattttc cgggtgttga
    94381 tccccgtgat gttcaggcca cactggcaga actcaccgcc gtgaccattt ctgaacaagt
    94441 tttgttgagc ggtggctgcg aacgattgat ggtatgtggt ggaggtagtc gtaatccgct
    94501 actcatggcg cgtctggcgg cattactgcc aggcacagaa gtcaccacca ccgatgccgt
    94561 tggcattagt ggcgatgaca tggaagcatt ggctttcgct tggcttgcct ggcggacgct
    94621 ggcgggatta ccaggaaatc tgccttccgt cactggcgca agccaggaga cggttctggg
    94681 ggctattttc cccgccaact cgtgatatca gaattaactg aattttttct tatcgtgtct
    94741 gccgttacac ttggaagatt aaggaggata tttgccctcc ggaccaggaa agtcttcggg
    94801 atatgactat gaaaaaactg ttaattatca ttttgccggt ccttctctcc ggctgtagcg
    94861 cctttaatca actggttgag cgaatgcaaa ccgatacgct ggaataccag tgtgatgaaa
    94921 aaccgttgac ggtcaaactg aataatccgc gccaggaagt cagttttgtt tacgataatc
    94981 aactactgca tctcaaacag ggcctttcag cctctggcgc gcgttactcc gacgggatct
    95041 atgttttctg gtcgaaaggt gaggaagcga ctgtctataa acgcgaccgc atcgtcttga
    95101 ataactgtca gttacaaaat ccacagcgtt gagatttttc caggggcggc gcacaatagc
    95161 gtcacccaca gacaatccgt aaagaaaacc atgtctgata acgacgaatt gcagcaaatc
    95221 gcgcatctgc gccgtgaata caccaaaggc gggttacgcc gccgcgatct tcccgccgat
    95281 ccattaaccc tttttgaacg ttggctctct caggcttgtg aagccaaact ggcggaccct
    95341 accgcgatgg tggtcgctac cgtggatgaa catggtcagc cttatcagcg catcgtttta
    95401 ctcaaacatt acgacgaaaa aggcatggtg ttttacacca acctcggtag ccgtaaagca
    95461 catcaaattg aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac ccttgaacgc
    95521 caggtgatgg tgatcggtaa agcagaacga ctttcgactc tcgaagtgat gaaatatttt
    95581 catagccgcc cgcgtgatag ccagattggt tcatgggttt cgaagcagtc cagtcgcatt
    95641 tctgcccgcg gtatccttga aagtaaattc ctggagctga agcagaagtt tcaacaaggc
    95701 gaagtgccat tgccgagctt ttggggcggt tttcgcgtca gccttgaaca gattgagttc
    95761 tggcagggcg gtgagcatcg cctgcatgac cgctttttgt accagcgtga aaatgatgcg
    95821 tggaagattg atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta atcgctggta
    95881 ctcctgattc tggcacttta ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg
    95941 gcaaaggtgc agtcgttata tacatggaga ttttgatggc aagcagtaac ttgattaaac
    96001 aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgtta gcagagcgac
    96061 tggcgcaagg cccgatcgcg ctctattgcg gcttcgatcc taccgctgac agcttgcatt
    96121 tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc cacaagccgg
    96181 ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa gctgccgagc
    96241 gtaagctgaa caccgaagaa actgttcagg agtgggtgga caaaatccgc aagcaggttg
    96301 ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggcgaac aactatgact
    96361 ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac ttctccgtta
    96421 accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat caggggattt
    96481 cgttcactga gttttcctac aacctgttgc agggttatga cttcgcctgt ctgaacaaac
    96541 agtacggtgt ggtgctgcaa attggtggtt ctgatcagtg gggtaacatc acttctggta
    96601 tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt ccgctgatca
    96661 ccaaagcaga tggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga
    96721 agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cactgcggat gccgacgttt
    96781 accgcttcct gaagttcttc acctttatga gcattgaaga gatcaacgcc ctggaagaag
    96841 aagataaaaa cagcggtaaa gcaccgcgcg cccagtatgt actggcggag caggtgactc
    96901 gtctggttca cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa tgcctgttca
    96961 gcggttcttt gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag gacggcgtac
    97021 cgatggttga gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat tctgaactgc
    97081 aaccttcccg tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc attaacggtg
    97141 aaaaacagtc cgatcctgaa tacttcttta aagaagaaga tcgtctgttt ggtcgtttta
    97201 ccttactgcg tcgcggcaaa aagaattact gtctgatttg ctggaaataa tgcattaagt
    97261 ggaaaggggg agtgagaaat cactccccct ggtttttata cagggaacat gatgaaaaat
    97321 attctcgcta tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc ggcagagttt
    97381 ccgatgcgcc gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt ttctaatcac
    97441 acccaatacg gcaaatggac tggctgcgtg atgccgccca gccatttaac cgaaattgtg
    97501 caaggcattg ccgccattga taaattacac acctgtgatg ccgtattaag tggctatctg
    97561 ggatcggcgg agcagggtga acatatcctc ggtatcgtcc gtcaggtgaa agcagcgaat
    97621 ccgcaggcga aatatttttg cgatccggta atgggtcatc cagaaaaagg ctgtatcgtt
    97681 gcaccgggtg tcgcagagtt tcatgtgcgg cacggtttgc ctgccagcga tatcattgcg
    97741 ccgaatcttg ttgagctgga aatactctgt gagcatccgg taaataacgt cgaagaagcg
    97801 gttctggcag cgcgcgaact cattgcgcaa gggccacaaa ttgtattggt taaacacctg
    97861 gcgcgagctg gctacagccg tgaccgtttt gaaatgttgc tggtcaccgc cgatgaagcc
    97921 tggcatatca gccgtccact ggtggatttt ggtatgcgcc agccggtagg tgttggtgat
    97981 gtgacgagcg gtttactgct ggtgaaactg cttcaggggg caacgctgca ggaggccctg
    98041 gaacatgtga ccgctgcagt ctacgaaatc atggtgacca ccaaagcaat gcaggaatat
    98101 gagttgcaag tggttgctgc tcaggatcgt attgccaaac cagaacatta cttcagcgca
    98161 acaaagctct gaaatatctc atttcggccc acaacgaact gtgggccgac ggctattact
    98221 ttaagccttc cgctgacagc gcgtcttgta cttccggacg ttcagccata cgttgcataa
    98281 atgccgcaat gtgctctaac ccttccagat tcagtttcac cgcgtatgcc cagcgcagta
    98341 ccgtaaacag ataggcatca gcaattgtaa atctttgccc gcagatccag tgctcatcct
    98401 tcagtgcctc gttcacatat tgcagtttct tctccagctg cgcgcgaact gtcggtttgt
    98461 actcttccgg tgtatcaggg cgaaacagag gtgtgaaacc tttatgcagc tcggtggcga
    98521 tgtaattcag ccattcgatg gttttatagc gggaaatact gtttaccggt gccagcaact
    98581 ggcggtcggg gacgctgtcg gcaagatact gcataatcgc tacaccttcc gtcagcaaag
    98641 taccgtcatc cagcagcaat gcaggtacct gtcccttagg gttaacggca aagtaatcgt
    98701 caccgttttc gagacgtttt ttcattaaat ccacactgac gagcgtaaaa tcctttccgc
    98761 tctcacgcag ggtgatatgg gaagcgagag agcaggcgcc cggtttgtag aacaatttca
    98821 tcggtaactc cttttgtctg tggtttaaag tatggtagtg cgttgctgaa caaaaaaaaa
    98881 gccgctaacg aaaagttagc ggcttgtaaa agagtttccc tgaattttac gctacggctg
    98941 ctttcgccgc tttgtctgca gcgtcatcct gcgtcatgcg gtgcagtttc ggcgcggtca
    99001 gcagcatcag tactgcaata acggcagtag cgacaccaat ctgcaagaat acgcgaccat
    99061 agacttccag tgacatcagc ggatcggtaa cgttatccgg cacagccatc atacccgcaa
    99121 cataaccacc aatcaggttt gcaccggcag tggtcaggaa ccagctaccc ataatgaagc
    99181 ccatcagacg ctgcggaacg agttgtgcaa ccattgccag acccagacca gagatcatca
    99241 gttccccgat actctgcagg ccatagctcg cgaccagcca gcttacagac acgatacctg
    99301 cgtcagacgc gaatttcgca cccagcggca gaatcaggaa cgcaccagaa cacatcacca
    99361 taccgattgc aaacttggtt ggcatcggca gggtatcgcc catcttgtta tagatagcgg
    99421 ccagaatcgg gctaccaatg atgatccaga acgggttcag tgcctgatac tgctcaggtt
    99481 ctacggccag gcccagaatg gtatgttcaa cgttacgaat cgcaaagaag ttcagtgacg
    99541 ttggcatctg gctgtacagc acgaagaaga taatggcttc gagcatcagg atgaaggcaa
    99601 cgatcatttt acgacgcgca gcacctttca tcgcgaaggc ttctttaccg aagataacca
    99661 cgataccgaa ggcaacaacg cccagcgcca tacgcgcaac ttcctgattg tgcagcagcc
    99721 aggtggcgat agcgatcagt gccacaacac caataatggt cagcagcagg ttacggtagt
    99781 tgataggctc gaagtctggt tttgaaccgt actgtttaac ccagcgttgg cagaaggcga
    99841 agttaacgat agtgatcagc aggcctacaa cgctcaacgc aaacgcaaca ctccagccgt
    99901 atttcgcggc cagccacggg gtggcgatca tagagaagaa agagccgatg ttgacggaca
    99961 tgtagtacat ggtgaatgca ccgtccagac gcgggtcgtt tttctcatag catgtagaaa
   100021 gcagagaaga cgggttagct ttaaacaggc cgttaccgac cgcaatagcc gccataccca
   100081 tataaacgat accggcgtcg tgaccagacc aggcaaccag agcataacca atcgccagca
   100141 caatagcgcc gagcataatt acgcgtttag tacccagtac cttgtcacct aaccagccgc
   100201 cgatagcgac cagaccataa accagggcac taaaggaaga gaaaagggtg attgaatccg
   100261 cttcagacat acccagttgt ttaaccaggt aaacagccat aattccttgt aggccgtaat
   100321 aaccaaaacg ttcccataac tcaatcgaga agatgagata gaacgccttc ggttgtttga
   100381 aagcgttcaa actgacgctt tcagttggtt tttggtttgc agtggacact tttacctctt
   100441 tttttacatc ccatattaac gggggtgctt tctcgcgtta tgtctaaaaa acatacgcgc
   100501 aattgttata gtgggagggg aaacggcagg taatgttcac tatcttgcac tttcagacaa
   100561 gtcctttgta ataatctgtt acatataact gaggcgggga attcaaccat tccgggaaat
   100621 gttatccagc gttaaatttt tcaaaaaacg taacggcaga ttttttcact gcattgagag
   100681 ggggatgatg gcttgacggc gatatgttct gctatttagc gtttttccta gtgagatagt
   100741 ccagtttctt aaaaatagcc agtgtaatgt tttgtaggtc aatatttgtt gcgtgtgtaa
   100801 atttaaatga ttttaacctg attcttgcaa tgtttttaca ttgccacagc atcgtgatct
   100861 tgatcactaa tgaatagaaa atttcgctat taaaaaaaca attaacctgc atttttggtt
   100921 atccgcaaac ggattacctg atgataacgc taagcaatgg catcattgac gttaaaaaga
   100981 tgttttaaaa gaggataaag aaaggttatc aatggggtaa tcggtgttac cccttttctt
   101041 cagatgtcaa ctttctcttt gtattcacaa agatcttcaa taatacaaga gccacagcgg
   101101 ggcttgcggg caatgcaggt ataacgcccg tgcaggatca accaatggtg gcagtcgact
   101161 ttaaactctg ccggaaccac tttcagtagc ttttcttcta cctgttcgac gtttttcccc
   101221 ggcgcaaatt gagtacgatt acaaacgcgg aaaatgtgcg tgtcgacagc aatagtcggc
   101281 cagccgaatg cagtgtttaa tacgacgttg gctgttttac gacctacgcc gggcagggct
   101341 tcaagcgcag cacgatcttc cggaacctcg ctattatgct gctccagtaa gatacggcag
   101401 gttttgatga tattttctgc tttgctgtta taaagcccaa tcgttttgat ataggttttc
   101461 accccttcaa cgcccagttc aagcatcgct gcaggcgtat tcgccaccgg gtagagtttc
   101521 gccgtcgcct tattaacact gacatcggtt gcctgagcgg aaagcagtac ggcaatcagc
   101581 aattcaaaag gcgaactgaa attaagctca gtggtgggat gaggattgtt ctcacgcagg
   101641 cgagtgagga tctccaggcg ttttgctttg ttcatcagac attccctgtt tcaccgtttg
   101701 gcaatgcacg ttcggcagct gcttcagtac ggcgcttttt cattctttca tcaatcaggt
   101761 attttcctgc cagcatcagt cccaggccaa taaatgcacc tggtggcagc atcgccagca
   101821 ggaaagggga gtcggtgtgg aaaatctcca cgcgtaatac ttttgcccag ctacctaaca
   101881 gcgcatctgc accgtcaaac aatgtgccat tgccgataat ttcgcgtagt gaacccagca
   101941 cgaacatggc gcaggttgcg cccataccaa ttgaaaagcc gtccagtgcc gaaagcgccg
   102001 gacctttttt ggcagcgaag gcttcagcgc ggcccacaac gatacagtta gtgacaatca
   102061 gcggaataaa aatccctaat gattgataca ggccaaaggc gtaggcgttg atcagcatct
   102121 gtacagcgct gaccaccgag gcgatgatca tcacgtaaat gggaatgcgg atctcggctg
   102181 gcgtccagtg acgcagcgtc gaaatggtca ggttggtcag cgtcagtacc agcgtagtcg
   102241 caagtcctaa acccagagcg ttagtggcag tagacgtgac agccaacaga ggacaaaggc
   102301 cgagcaactg gaccagcgca gagttgtttt tccacaaccc ctgaacaata acgtctttaa
   102361 tttcgctcac gctttattct ccacaggcag gaagttgaga aagttgttcc ggtaacgtct
   102421 gagcgtacaa tccggcgcgt tttaccgcat taaccaccgc gcggggagta atcgtcgcgc
   102481 cggtgaactg gtcgaaatta ccaccatctt tcttcaccgc ccagtgcgca tcatccgcac
   102541 cactgatttt tttacccgca aaatgggtga tccagtcaga aaggcgcagt tcgattttat
   102601 cgccaagccc tggcgtttcg tggtgctctg tcacgcgcgt gccaagtacc gtgccgttaa
   102661 aatcggctcc caccagcagc tgaatcgcac cggaatagcc atctggcgcg gttgcttcca
   102721 gaacggcggc taccggtttg tcatcctgtt tggcgatgta aacccgatgc tcacctttac
   102781 ctaactctgg cgcagttacc agatagcaac tctgtgccag cgcattgtta tagcgttcgg
   102841 ctggcagcac ctgatcaaat aaagcctttt gttgcagact ggcctgttca gcaatcgtcg
   102901 ttttggtcat ctggttgatg gccgcagtta accctgttga acccgctgca aatagcgcca
   102961 acgtaatgcc gtgttttcgg atagttttca gcatggttta ccctttgcga tggccgtaga
   103021 cgcgcggacg cgtgtagtaa tcgatcagag gaaccgtgat gttcgccagc aggacggcaa
   103081 aagccacgcc gtcaggatag ccgccgaaac tgcggatcaa ccagactaat aagcccgcaa
   103141 gcgcgccgaa aataagacga ccacgattgg tcgtagaagc ggtaaccggg tcagtcaaaa
   103201 taaagaatgc gccgagcatg gtcgctccag acagcagatg aatttgcggt gctgccagtg
   103261 tttctggtga gaacaaccag cccaacgttg cgcataacgc cagcgttact aagaagctga
   103321 ggggaatatg ccagcgaatc gctttctgcc atagcaacca tacgccgcca gccagccagg
   103381 cgagatttac ccattgccaa ccagcgcccg ccagaatacc gctgtagatc ggatattgca
   103441 taatctgttc aaccgaatga ccggcacgga cagaggtttt aaatgtatcc agcggtgtcg
   103501 cctgactaat gccatcaata cctaagcgta gtgtgttcat atcaccacca ctggcggtat
   103561 gcccgctgaa aataacctgt atggcgtcga taaaaccagg gatgttgacc gcaatttcat
   103621 gtggcggtaa ccagctggtc atctgcaccg ggaaggagat cagtaagacc acataaccaa
   103681 tcattgccgg attaaacggg ttttgcccca gaccgccata caactgttta gcgatgataa
   103741 ccgcaaacac cgtacccagc acgaccatcc accatggcgc gaggggggga atacttaccg
   103801 ccagcaataa gcctgtcagc aatgctgagt tatctttcaa cgtggcggct accgactgct
   103861 tgcgtagttt gagtacgaga gcttcggcta acagagcact aaccgatgcc aacaggatct
   103921 gaacgagagt accccaacca aaaaaccaca gttgcgctgc gattcctggc acggctgcga
   103981 gcaacaccaa cagcataatg cgcgatgtct ggcgctggtt atgggtataa ggggagctag
   104041 ctattctgaa taccatttag tcctcgttta caaccttttg ctggacggct tttttggcct
   104101 gaacgcgggc aatagccgcg gcaactgccg ctttgcgcgg atcaacctgt tcttctggtt
   104161 ctgcattagc ctgttgctgt tccagcttgc gtgctttggc gcgtgcaata gccgctgcga
   104221 cggcagcttt gcgcggatca acctgttctt ctggctccgc attagcctgt tgctgttcca
   104281 gtttgcgcgc tttggcacgg g
//
LOCUS       DAZFBR010000002        98390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFBR010000002 DAZFBR010000000
VERSION     DAZFBR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 98390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.002"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(153..1061)
                     /gene="abgR"
                     /locus_tag="ACHJZ2_000106"
     CDS             complement(153..1061)
                     /gene="abgR"
                     /locus_tag="ACHJZ2_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4566307.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            1237..2547
                     /gene="abgA"
                     /locus_tag="ACHJZ2_000107"
     CDS             1237..2547
                     /gene="abgA"
                     /locus_tag="ACHJZ2_000107"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO4566308.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            2547..3992
                     /gene="abgB"
                     /locus_tag="ACHJZ2_000108"
     CDS             2547..3992
                     /gene="abgB"
                     /locus_tag="ACHJZ2_000108"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO4566309.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            4029..5555
                     /gene="abgT"
                     /locus_tag="ACHJZ2_000109"
     CDS             4029..5555
                     /gene="abgT"
                     /locus_tag="ACHJZ2_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO4566310.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            5566..6081
                     /gene="ogt"
                     /locus_tag="ACHJZ2_000110"
     CDS             5566..6081
                     /gene="ogt"
                     /locus_tag="ACHJZ2_000110"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO4566311.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            6276..7028
                     /gene="fnr"
                     /locus_tag="ACHJZ2_000111"
     CDS             6276..7028
                     /gene="fnr"
                     /locus_tag="ACHJZ2_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO4566312.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKNGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            7180..8130
                     /gene="uspE"
                     /locus_tag="ACHJZ2_000112"
     CDS             7180..8130
                     /gene="uspE"
                     /locus_tag="ACHJZ2_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO4566313.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(8180..8437)
                     /gene="ynaJ"
                     /locus_tag="ACHJZ2_000113"
     CDS             complement(8180..8437)
                     /gene="ynaJ"
                     /locus_tag="ACHJZ2_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO4566314.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            8681..9712
                     /gene="ynaI"
                     /locus_tag="ACHJZ2_000114"
     CDS             8681..9712
                     /gene="ynaI"
                     /locus_tag="ACHJZ2_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO4566315.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIVISAVGGIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(9763..11376)
                     /gene="mppA"
                     /locus_tag="ACHJZ2_000115"
     CDS             complement(9763..11376)
                     /gene="mppA"
                     /locus_tag="ACHJZ2_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO4566316.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDVHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(11712..12611)
                     /gene="pgrR"
                     /locus_tag="ACHJZ2_000116"
     CDS             complement(11712..12611)
                     /gene="pgrR"
                     /locus_tag="ACHJZ2_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO4566317.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            12749..13669
                     /locus_tag="ACHJZ2_000117"
     CDS             12749..13669
                     /locus_tag="ACHJZ2_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4566318.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            13669..14599
                     /locus_tag="ACHJZ2_000118"
                     /pseudo
     CDS             13669..14599
                     /locus_tag="ACHJZ2_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            14620..15258
                     /locus_tag="ACHJZ2_000119"
     CDS             14620..15258
                     /locus_tag="ACHJZ2_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4566319.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            15549..16277
                     /gene="mpaA"
                     /locus_tag="ACHJZ2_000120"
     CDS             15549..16277
                     /gene="mpaA"
                     /locus_tag="ACHJZ2_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO4566320.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(16252..17217)
                     /gene="ycjG"
                     /locus_tag="ACHJZ2_000121"
     CDS             complement(16252..17217)
                     /gene="ycjG"
                     /locus_tag="ACHJZ2_000121"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO4566321.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            17336..17842
                     /gene="tpx"
                     /locus_tag="ACHJZ2_000122"
     CDS             17336..17842
                     /gene="tpx"
                     /locus_tag="ACHJZ2_000122"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO4566322.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(17886..19427)
                     /gene="tyrR"
                     /locus_tag="ACHJZ2_000123"
     CDS             complement(17886..19427)
                     /gene="tyrR"
                     /locus_tag="ACHJZ2_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO4566323.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(19575..20636)
                     /gene="ycjF"
                     /locus_tag="ACHJZ2_000124"
     CDS             complement(19575..20636)
                     /gene="ycjF"
                     /locus_tag="ACHJZ2_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO4566324.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(20633..22030)
                     /gene="ycjX"
                     /locus_tag="ACHJZ2_000125"
     CDS             complement(20633..22030)
                     /gene="ycjX"
                     /locus_tag="ACHJZ2_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO4566325.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQSAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            22185..23183
                     /gene="ycjW"
                     /locus_tag="ACHJZ2_000126"
     CDS             22185..23183
                     /gene="ycjW"
                     /locus_tag="ACHJZ2_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4566326.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            complement(23294..24199)
                     /gene="ompG"
                     /locus_tag="ACHJZ2_000127"
     CDS             complement(23294..24199)
                     /gene="ompG"
                     /locus_tag="ACHJZ2_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO4566327.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(24244..25326)
                     /locus_tag="ACHJZ2_000128"
     CDS             complement(24244..25326)
                     /locus_tag="ACHJZ2_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4566328.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(25340..25999)
                     /gene="pgmB"
                     /locus_tag="ACHJZ2_000129"
     CDS             complement(25340..25999)
                     /gene="pgmB"
                     /locus_tag="ACHJZ2_000129"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO4566329.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(25996..28263)
                     /gene="ycjT"
                     /locus_tag="ACHJZ2_000130"
     CDS             complement(25996..28263)
                     /gene="ycjT"
                     /locus_tag="ACHJZ2_000130"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO4566330.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(28260..29315)
                     /gene="ycjS"
                     /locus_tag="ACHJZ2_000131"
     CDS             complement(28260..29315)
                     /gene="ycjS"
                     /locus_tag="ACHJZ2_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4566331.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(29325..30113)
                     /gene="ycjR"
                     /locus_tag="ACHJZ2_000132"
     CDS             complement(29325..30113)
                     /gene="ycjR"
                     /locus_tag="ACHJZ2_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO4566332.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            complement(30131..31183)
                     /gene="ycjQ"
                     /locus_tag="ACHJZ2_000133"
     CDS             complement(30131..31183)
                     /gene="ycjQ"
                     /locus_tag="ACHJZ2_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO4566333.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(31214..32056)
                     /gene="ycjP"
                     /locus_tag="ACHJZ2_000134"
     CDS             complement(31214..32056)
                     /gene="ycjP"
                     /locus_tag="ACHJZ2_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4566334.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(32043..32924)
                     /gene="ycjO"
                     /locus_tag="ACHJZ2_000135"
     CDS             complement(32043..32924)
                     /gene="ycjO"
                     /locus_tag="ACHJZ2_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4566335.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(32945..34237)
                     /gene="ycjN"
                     /locus_tag="ACHJZ2_000136"
                     /pseudo
     CDS             complement(32945..34237)
                     /gene="ycjN"
                     /locus_tag="ACHJZ2_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(34251..35929)
                     /gene="ycjM"
                     /locus_tag="ACHJZ2_000137"
                     /pseudo
     CDS             complement(34251..35929)
                     /gene="ycjM"
                     /locus_tag="ACHJZ2_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(36139..36453)
                     /gene="pspE"
                     /locus_tag="ACHJZ2_000138"
     CDS             complement(36139..36453)
                     /gene="pspE"
                     /locus_tag="ACHJZ2_000138"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO4566336.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(36528..36749)
                     /gene="pspD"
                     /locus_tag="ACHJZ2_000139"
     CDS             complement(36528..36749)
                     /gene="pspD"
                     /locus_tag="ACHJZ2_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO4566337.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(36758..37117)
                     /gene="pspC"
                     /locus_tag="ACHJZ2_000140"
     CDS             complement(36758..37117)
                     /gene="pspC"
                     /locus_tag="ACHJZ2_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO4566338.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(37117..37341)
                     /gene="pspB"
                     /locus_tag="ACHJZ2_000141"
     CDS             complement(37117..37341)
                     /gene="pspB"
                     /locus_tag="ACHJZ2_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO4566339.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(37395..38063)
                     /gene="pspA"
                     /locus_tag="ACHJZ2_000142"
     CDS             complement(37395..38063)
                     /gene="pspA"
                     /locus_tag="ACHJZ2_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO4566340.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            38230..39207
                     /gene="pspF"
                     /locus_tag="ACHJZ2_000143"
     CDS             38230..39207
                     /gene="pspF"
                     /locus_tag="ACHJZ2_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO4566341.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(39338..40603)
                     /gene="puuE"
                     /locus_tag="ACHJZ2_000144"
     CDS             complement(39338..40603)
                     /gene="puuE"
                     /locus_tag="ACHJZ2_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO4566342.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(40641..41921)
                     /gene="puuB"
                     /locus_tag="ACHJZ2_000145"
     CDS             complement(40641..41921)
                     /gene="puuB"
                     /locus_tag="ACHJZ2_000145"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO4566343.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(41923..43410)
                     /gene="puuC"
                     /locus_tag="ACHJZ2_000146"
     CDS             complement(41923..43410)
                     /gene="puuC"
                     /locus_tag="ACHJZ2_000146"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO4566344.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(43560..44117)
                     /gene="puuR"
                     /locus_tag="ACHJZ2_000147"
     CDS             complement(43560..44117)
                     /gene="puuR"
                     /locus_tag="ACHJZ2_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO4566345.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(44144..44908)
                     /gene="puuD"
                     /locus_tag="ACHJZ2_000148"
     CDS             complement(44144..44908)
                     /gene="puuD"
                     /locus_tag="ACHJZ2_000148"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO4566346.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            45120..46538
                     /gene="puuA"
                     /locus_tag="ACHJZ2_000149"
     CDS             45120..46538
                     /gene="puuA"
                     /locus_tag="ACHJZ2_000149"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO4566347.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            46687..46851
                     /locus_tag="ACHJZ2_000150"
     CDS             46687..46851
                     /locus_tag="ACHJZ2_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566348.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            46841..48226
                     /gene="puuP"
                     /locus_tag="ACHJZ2_000151"
     CDS             46841..48226
                     /gene="puuP"
                     /locus_tag="ACHJZ2_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO4566349.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            48360..48605
                     /gene="ymjA"
                     /locus_tag="ACHJZ2_000152"
     CDS             48360..48605
                     /gene="ymjA"
                     /locus_tag="ACHJZ2_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO4566350.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            48918..50561
                     /gene="sapA"
                     /locus_tag="ACHJZ2_000153"
     CDS             48918..50561
                     /gene="sapA"
                     /locus_tag="ACHJZ2_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO4566351.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            50558..51523
                     /gene="sapB"
                     /locus_tag="ACHJZ2_000154"
     CDS             50558..51523
                     /gene="sapB"
                     /locus_tag="ACHJZ2_000154"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO4566352.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            51510..52400
                     /gene="sapC"
                     /locus_tag="ACHJZ2_000155"
     CDS             51510..52400
                     /gene="sapC"
                     /locus_tag="ACHJZ2_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO4566353.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            52400..53392
                     /gene="sapD"
                     /locus_tag="ACHJZ2_000156"
     CDS             52400..53392
                     /gene="sapD"
                     /locus_tag="ACHJZ2_000156"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO4566354.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            53394..54200
                     /gene="sapF"
                     /locus_tag="ACHJZ2_000157"
     CDS             53394..54200
                     /gene="sapF"
                     /locus_tag="ACHJZ2_000157"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO4566355.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(54252..55415)
                     /locus_tag="ACHJZ2_000158"
     CDS             complement(54252..55415)
                     /locus_tag="ACHJZ2_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO4566356.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(55424..56797)
                     /locus_tag="ACHJZ2_000159"
     CDS             complement(55424..56797)
                     /locus_tag="ACHJZ2_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO4566357.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQRAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(56801..59909)
                     /locus_tag="ACHJZ2_000160"
                     /pseudo
     CDS             complement(56801..59909)
                     /locus_tag="ACHJZ2_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(59909..61030)
                     /locus_tag="ACHJZ2_000161"
     CDS             complement(59909..61030)
                     /locus_tag="ACHJZ2_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO4566358.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHSGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(61179..61745)
                     /locus_tag="ACHJZ2_000162"
     CDS             complement(61179..61745)
                     /locus_tag="ACHJZ2_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO4566359.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            62223..63011
                     /gene="fabI"
                     /locus_tag="ACHJZ2_000163"
     CDS             62223..63011
                     /gene="fabI"
                     /locus_tag="ACHJZ2_000163"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO4566360.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            63155..64282
                     /gene="yciW"
                     /locus_tag="ACHJZ2_000164"
     CDS             63155..64282
                     /gene="yciW"
                     /locus_tag="ACHJZ2_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO4566361.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            64350..66284
                     /gene="rnb"
                     /locus_tag="ACHJZ2_000165"
     CDS             64350..66284
                     /gene="rnb"
                     /locus_tag="ACHJZ2_000165"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO4566362.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(66403..66465)
                     /locus_tag="ACHJZ2_000166"
                     /pseudo
     CDS             complement(66403..66465)
                     /locus_tag="ACHJZ2_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            66519..68504
                     /gene="pdeR"
                     /locus_tag="ACHJZ2_000167"
     CDS             66519..68504
                     /gene="pdeR"
                     /locus_tag="ACHJZ2_000167"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4566363.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDILRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTAY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(68486..68614)
                     /gene="ymiD"
                     /locus_tag="ACHJZ2_000168"
     CDS             complement(68486..68614)
                     /gene="ymiD"
                     /locus_tag="ACHJZ2_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO4566364.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            68652..68825
                     /gene="yciZ"
                     /locus_tag="ACHJZ2_000169"
     CDS             68652..68825
                     /gene="yciZ"
                     /locus_tag="ACHJZ2_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO4566365.1"
                     /translation="MSEFDAQRVAERIDIVLDILVADDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            68915..69664
                     /gene="yciT"
                     /locus_tag="ACHJZ2_000170"
     CDS             68915..69664
                     /gene="yciT"
                     /locus_tag="ACHJZ2_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO4566366.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            69933..70151
                     /gene="osmB"
                     /locus_tag="ACHJZ2_000171"
     CDS             69933..70151
                     /gene="osmB"
                     /locus_tag="ACHJZ2_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO4566367.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(70277..70603)
                     /gene="yciH"
                     /locus_tag="ACHJZ2_000172"
     CDS             complement(70277..70603)
                     /gene="yciH"
                     /locus_tag="ACHJZ2_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO4566368.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(70603..71340)
                     /gene="pyrF"
                     /locus_tag="ACHJZ2_000173"
     CDS             complement(70603..71340)
                     /gene="pyrF"
                     /locus_tag="ACHJZ2_000173"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO4566369.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(71533..72702)
                     /gene="lapB"
                     /locus_tag="ACHJZ2_000174"
     CDS             complement(71533..72702)
                     /gene="lapB"
                     /locus_tag="ACHJZ2_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO4566370.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(72709..73017)
                     /gene="lapA"
                     /locus_tag="ACHJZ2_000175"
     CDS             complement(72709..73017)
                     /gene="lapA"
                     /locus_tag="ACHJZ2_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO4566371.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            complement(73166..73930)
                     /gene="pgpB"
                     /locus_tag="ACHJZ2_000176"
     CDS             complement(73166..73930)
                     /gene="pgpB"
                     /locus_tag="ACHJZ2_000176"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO4566372.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            74100..74690
                     /gene="ribA"
                     /locus_tag="ACHJZ2_000177"
     CDS             74100..74690
                     /gene="ribA"
                     /locus_tag="ACHJZ2_000177"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO4566373.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(74754..77429)
                     /gene="acnA"
                     /locus_tag="ACHJZ2_000178"
     CDS             complement(74754..77429)
                     /gene="acnA"
                     /locus_tag="ACHJZ2_000178"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO4566374.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(77593..77688)
                     /gene="ymiC"
                     /locus_tag="ACHJZ2_000179"
     CDS             complement(77593..77688)
                     /gene="ymiC"
                     /locus_tag="ACHJZ2_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO4566375.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(77802..77969)
                     /gene="yciX"
                     /locus_tag="ACHJZ2_000180"
     CDS             complement(77802..77969)
                     /gene="yciX"
                     /locus_tag="ACHJZ2_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO4566376.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(77972..78112)
                     /gene="ymiA"
                     /locus_tag="ACHJZ2_000181"
     CDS             complement(77972..78112)
                     /gene="ymiA"
                     /locus_tag="ACHJZ2_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO4566377.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(78431..79405)
                     /gene="cysB"
                     /locus_tag="ACHJZ2_000182"
     CDS             complement(78431..79405)
                     /gene="cysB"
                     /locus_tag="ACHJZ2_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO4566378.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(79615..82212)
                     /gene="topA"
                     /locus_tag="ACHJZ2_000183"
     CDS             complement(79615..82212)
                     /gene="topA"
                     /locus_tag="ACHJZ2_000183"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO4566379.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            82592..82843
                     /gene="yciN"
                     /locus_tag="ACHJZ2_000184"
     CDS             82592..82843
                     /gene="yciN"
                     /locus_tag="ACHJZ2_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO4566380.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(82879..83928)
                     /gene="sohB"
                     /locus_tag="ACHJZ2_000185"
     CDS             complement(82879..83928)
                     /gene="sohB"
                     /locus_tag="ACHJZ2_000185"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO4566381.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            84148..84906
                     /gene="yciK"
                     /locus_tag="ACHJZ2_000186"
     CDS             84148..84906
                     /gene="yciK"
                     /locus_tag="ACHJZ2_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO4566382.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            84903..85493
                     /gene="cobO"
                     /locus_tag="ACHJZ2_000187"
     CDS             84903..85493
                     /gene="cobO"
                     /locus_tag="ACHJZ2_000187"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO4566383.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(85533..86405)
                     /gene="rluB"
                     /locus_tag="ACHJZ2_000188"
     CDS             complement(85533..86405)
                     /gene="rluB"
                     /locus_tag="ACHJZ2_000188"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO4566384.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(86618..88201)
                     /locus_tag="ACHJZ2_000189"
     CDS             complement(86618..88201)
                     /locus_tag="ACHJZ2_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO4566385.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTSVD
                     VLRMQKFYLPRKKSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMIC
                     WLLPFLFCSLELLFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMP
                     AGYFSAVFLTGYLTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGE
                     TRKLDYALLGWAVSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTS
                     LLGRSYSGGAAGGGGGGGW"
     gene            complement(88229..88849)
                     /gene="yciO"
                     /locus_tag="ACHJZ2_000190"
     CDS             complement(88229..88849)
                     /gene="yciO"
                     /locus_tag="ACHJZ2_000190"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO4566386.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(88846..89727)
                     /gene="rnm"
                     /locus_tag="ACHJZ2_000191"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(88846..89727)
                     /gene="rnm"
                     /locus_tag="ACHJZ2_000191"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO4566387.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKVVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            89865..89909
                     /gene="trpL"
                     /locus_tag="ACHJZ2_000192"
     CDS             89865..89909
                     /gene="trpL"
                     /locus_tag="ACHJZ2_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO4566388.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    89886..89979
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            90001..91563
                     /gene="trpE"
                     /locus_tag="ACHJZ2_000193"
     CDS             90001..91563
                     /gene="trpE"
                     /locus_tag="ACHJZ2_000193"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO4566389.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            91563..93158
                     /gene="trpD"
                     /locus_tag="ACHJZ2_000194"
     CDS             91563..93158
                     /gene="trpD"
                     /locus_tag="ACHJZ2_000194"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO4566390.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            93159..94520
                     /gene="trpCF"
                     /locus_tag="ACHJZ2_000195"
     CDS             93159..94520
                     /gene="trpCF"
                     /locus_tag="ACHJZ2_000195"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO4566391.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTREIAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLDKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            94532..95725
                     /gene="trpB"
                     /locus_tag="ACHJZ2_000196"
     CDS             94532..95725
                     /gene="trpB"
                     /locus_tag="ACHJZ2_000196"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO4566392.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            95725..96531
                     /gene="trpA"
                     /locus_tag="ACHJZ2_000197"
     CDS             95725..96531
                     /gene="trpA"
                     /locus_tag="ACHJZ2_000197"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO4566393.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            96912..97091
                     /gene="yciG"
                     /locus_tag="ACHJZ2_000198"
     CDS             96912..97091
                     /gene="yciG"
                     /locus_tag="ACHJZ2_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO4566394.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            97177..97677
                     /gene="yciF"
                     /locus_tag="ACHJZ2_000199"
     CDS             97177..97677
                     /gene="yciF"
                     /locus_tag="ACHJZ2_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4566395.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            97723..98229
                     /gene="yciE"
                     /locus_tag="ACHJZ2_000200"
     CDS             97723..98229
                     /gene="yciE"
                     /locus_tag="ACHJZ2_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4566396.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(98289..>98390)
                     /locus_tag="ACHJZ2_000201"
     CDS             complement(98289..>98390)
                     /locus_tag="ACHJZ2_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HFO4566397.1"
                     /translation="IDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT        24153 a        25737 c        24138 g        24362 t
ORIGIN      
        1 gtcactgaag aaaattggca actaaaggtt aaaaccgtta taacacagtc accggcgcag
       61 aggagacaat gccggattta agacgcggat gcactgctgt gtgtactgta gagtctggcg
      121 gatgtcgaca gactctattt ttttatgcag ttttaacttt gcagatagcc gcattctcgc
      181 cggaattgcg tgattaatga cgcggtcagt ggtgtctggc gactatcacg ccgctgaatc
      241 aaataatagg ccgctttcgg taaaatttcg ctaaccggca acatcaccag cccctgtccg
      301 tgcaagggat cgcagcccat ttcttcaggc agtatgctga gaaaatcgct ttttgccacc
      361 agactgatac aggctgagaa cgtctcgcag actacaccga cctgtggcgt ttgcgcctga
      421 tcgtcaagca attcactcaa ctgtttgtag tagctgccgt gtggcgtcgg cattgtccag
      481 ctgtaatcca gtaactgttt gatcgaacgg gcaccaatgg cggggtgtcc cgggcggcaa
      541 aagatcgcga attgcttttc cagtaatttc tcaaaagtaa attcgtggtc gtacggtccc
      601 tgataatagg tattgatggt gaaatccaat tctccctgac gcaattcatt aatcatcgac
      661 accagttgcc cttccataat gcgtactttt acctgcggat gctgctgatg aaaacgagat
      721 attacagctg gcatcagact gcgggaaata ctggccccca tgccgatatt aatctgccct
      781 gccagttgcc cttgtcgttg gcgaatatcc tcttgggctg cgcgcagttc ttcaagaata
      841 agactggcgt gctgataaaa actttcaccg gcatcagtta acgtcacgcc tttactacgg
      901 cgaaaaaaga gttgcgccgc taacccttct tccagctcct gaatagattt actcagtgcc
      961 ggttgcgaca tattcaacat tcggctcgct ccgcgaatgc tgccctgacg agccacttca
     1021 acaaaagccc gaatttgatg aatttttacc tgaaaagcca tgacgccacc gataaccgtt
     1081 atttatcaga ccaaagaaac tggcatctac tttaatgcag atgattgtgt cagggtaatt
     1141 tatgaacggt taaaactgtg aaaaatcagt tagtgataag taaaaactat cgctacgtga
     1201 accgggtcac actttttact gatgacggga aaggttatgg agtctttgaa tcaatttgtt
     1261 aattcgcttg ccccaaaatt atcgcactgg cgacgtgatt ttcatcacta tgcagagtct
     1321 ggctgggtgg aattccgcac tgccaccctt gttgcggaag aattgcagca gctcggctac
     1381 tcactggcgc tgggccgcga agtagttaat gaaaatagcc ggatgggact acctgatgaa
     1441 ctcactctac aacgcgaatt cgagcgcgct cgtcaacagg gtgcgctagc acaatggatt
     1501 gcggcttttg aaggtggttt cacgggtatc gtcgccaccc tggataccgg tcgccccggt
     1561 ccggtgatgg ctttccgtgt cgatatggac gcgctggatc tcagtgaaga gcaggatgtc
     1621 agccatcgcc cctaccgcga cggttttgcg tcatgtaacg ccggaatgat gcatgcctgt
     1681 ggtcatgatg ggcataccac cattgggctt gggctggcgc atacccttaa acaattcgag
     1741 tccggactac atggcgtcat caaactgatt tttcagcctg cagaggaagg tacgcgtggc
     1801 gcgcgggcga tggtcgatgc aggtgtcgta gatgatgttg attattttac tgccgtgcac
     1861 attggcactg gcgtacctgc gggcaccgtg gtgtgcggca gtgataattt tatggcaacc
     1921 accaaatttg acgcgcactt caccggtacc gccgctcacg caggcgcaaa accagaagac
     1981 ggttacaatg ccttgttggc ggcagcacaa gccactcttg cactgcatgc aatcgccccg
     2041 cacagcgaag gagcttccag agtaaacgtg ggcgttatgc aggcaggaag cggtcgtaac
     2101 gttgttcctg cctcggcgtt gctgaaagtg gaaacacgcg gggccagcga cgtcattaat
     2161 caatatgttt ttgaccgtgc tcagcaagcg attcagggcg cagcaaccat gtatggtgtc
     2221 ggcgttgaaa ctcgtctgat gggtgcagct accgccagtt ctccttcgcc gcaatgggtc
     2281 gcatggttgc aaagtcaggc ggctcaggtc gcgggggtca atcaggccat tgaacgtgtt
     2341 gaagcgcctg cgggttccga agatgccaca ttaatgatgg cccgcgtgca gcaacatcaa
     2401 gggcaagcct cctacgtggt gtttggcaca cagctggcgg caggtcatca caacgaaaaa
     2461 ttcgattttg acgagcaggt tctcgctatt gccgtcgaaa cgctggcgcg caccgcgctc
     2521 aattttccct ggacgcgagg tatctgatgc aggaaatcta tcgttttatc gacgatgcga
     2581 ttgaagccga tcgccaacgt tataccgata ttgccgatca aatctgggat catccagaaa
     2641 cacgttttga agagttctgg tcagcggagc atctggcttc ggcgctggaa tctgcaggct
     2701 tcaccgttac ccgcaacgta ggcaatatcc caaatgcctt tattgcttcg tttggtcaag
     2761 gcaaaccggt tatcgccctg ctgggagaat atgacgccct ggcaggttta agtcagcaag
     2821 caggttgcgc gcaacctaca tccgtgacgc ccggtgaaaa tggtcacggt tgcggacaca
     2881 atttgctggg aaccgccgcc tttgccgctg caatagccat caagaaatgg ctggaacaat
     2941 atgggcaagg cggcacagtg cgcttttatg gttgtcctgg cgaagaaggc ggctcgggta
     3001 aaacgttcat ggtccgcgag ggggtatttg atgatgtgga tgcggcactc acctggcacc
     3061 cggaagcctt tgccggtatg ttcaataccc gtacgctggc aaacattcag gcatcatggc
     3121 gctttaaagg gatcgcagca catgccgcga attcccctca tttgggacgc agcgcccttg
     3181 atgccgtaac gttgatgacc actggcacca acttcctcaa cgaacatatt attgaaaaag
     3241 cgcgcgtaca ctatgccatc acaaatagtg gcgggatctc gcccaacgtg gtccaggcgc
     3301 aggcagaagt gctttatctt atccgcgccc ccgaaatgac cgacgtgcag catatttatg
     3361 atcgggtcgc caaaatcgcc gaaggtgcgg cattgatgac cgaaaccacg gttgaatgcc
     3421 gcttcgacaa agcctgttcc agttatctcc cgaatcgcac cttagaaaat gccatgtacc
     3481 aggccctatc ccattttggt accccggaat ggaactccga agaactggct tttgcgaaac
     3541 aaattcaggc tacgcttacc tccaacgatc ggcaaaacag tctgaataat atcgccgcaa
     3601 ccggtggcga aaacggcaag gtttttgcac tacgtcatcg tgaaacggta ctggcgaatg
     3661 aagtcgctcc atatgccgcc accgataacg tgcttgcggc atcgactgat gtcggcgacg
     3721 tcagttggaa actgcctgtt gcccagtgtt tcagcccctg ttttgccgtc ggtacacccc
     3781 tacatacgtg gcaactggtt agccaggggc gaacatctat tgctcataaa ggaatgctgc
     3841 tggcggcgaa aactatggca gcaaccacac tcaatctctt cattgattca gggctattgc
     3901 aagaatgcca acaagagcat cagcaagtta cggacacgca accgtatcac tgccctatcc
     3961 cgaaaaacgt gacaccgtca cctttaaaat aacaacaaca acgcaaacac aacaaccgag
     4021 gaatgcccat gagtatgtca tccataccgt cgtcctccca atccgggaag ctctatggct
     4081 gggtcgaaag aattggtaac aaggttcccc atcctttttt gctctttatc tatttgatta
     4141 tcgtactcat ggtgacgacg gcaattttgt cggcctttgg cgtcagtgcg aaaaacccga
     4201 ccgatggtac gccggtggtg gtgaaaaacc tgctcagtgt ggaaggatta cactggtttt
     4261 tacccaatgt tattaaaaac tttagcggtt ttgctccact tggtgcgatc ctggcgctgg
     4321 ttttaggtgc cggtctggcg gagcgcgtcg gcttactgcc agcactaatg gttaaaatgg
     4381 catcgcatgt taatgcccgc tacgccagtt atatggtgct gtttattgct tttttcagcc
     4441 acatttcttc cgatgcggcg ttagtgatca tgccaccgat gggtgcgctg atttttctgg
     4501 cggtgggcag gcatccagtt gcaggtttac tggctgccat tgcaggcgta ggttgcggct
     4561 tcacggctaa tttactgatt gtcacaaccg acgtgttgct gtcggggatc agcacggaag
     4621 cggcagctgc gttcaatccg caaatgcacg tcagtgtaat tgataactgg tattttatgg
     4681 ccagctccgt agtcgtactg acgattgttg gcggcctgat aaccgacaaa atcatcgagc
     4741 cacggttagg tcaatggcag ggaaacagcg atgagaaact gcagacattg accgaaagtc
     4801 agcgttttgg tttacgcata gcaggtgtcg tatcgctact ttttattgct gcgattgcgc
     4861 tgatggtgat cccggaaaac gggatattgc gcgatccgat taatcacacc gtgatgccat
     4921 caccctttat taaaggtatc gtgccactga tcattctttt tttctttgtt gtctcgctgg
     4981 cttatggcat cgctacccgc acaattcgac gtcaggcgga tttaccgcat ttaatgattg
     5041 aaccgatgaa agagatggcg ggatttatcg tgatggtttt tcccctcgcc cagtttgtcg
     5101 ccatgtttaa ctggagcaac atggggaaat tcatcgccgt ggggctgacc gatatcctgg
     5161 aaagttcagg gcttagcggc atcccggcgt ttgtcggtct ggcgttgctt tcctctttct
     5221 tatgcatgtt tatcgccagc ggttccgcaa tctggtcgat tctggccccc attttcgtac
     5281 caatgtttat gctacttggc tttcacccgg catttgcgca aatcctcttt cgtattgccg
     5341 actcatccgt attgccttta gcgccagtat ctccttttgt tccactgttt cttggattcc
     5401 tgcaacgcta caaaccagac gcgaaactgg gtacttacta ttcgttagtc ttgccctatc
     5461 cgcttatctt tttggtggta tggctgctga tgttgctggc gtggtatctt gtgggcctgc
     5521 cgataggtcc gggtatttac ccacgtttgt cttaagagag aacggatgct gagattactt
     5581 gaagaaaaaa ttgccacacc actgggtcca ctgtgggtga tttgcgatga gcaatttcgc
     5641 ctgcgggcgg ttgaatggga agagtacagc gaacgcatgg tgcagctgct ggacatccat
     5701 tatcgcaaag aaggctatga gcgcatttct gccaccaatc caggcggttt aagcgacaag
     5761 cttcgtgaat attttgccgg taatcttagc attattgata cgcttcccac tgctacgggg
     5821 gggacgccat ttcagcgcga agtctggaaa acactacgca ctatcccctg cgggcaggta
     5881 atgcattacg gcgaactggc tgagcaattg ggccgtcctg gcgcggcgcg tgccgttggt
     5941 gcggcaaacg gatcgaatcc catcagcatc gtcgtacctt gccatcgggt tattggccga
     6001 aacggcacca tgaccggata tgcaggcgga gttcagcgaa aagagtggtt attgcgccac
     6061 gaaggttatc ttttgctgta aacattaaac aatttgtgcc agcttgttca cacttttatg
     6121 taaagttacc cttaacaact taagggtttt caaatagata gacatatatt tacatctaat
     6181 atcggaattc tctgctgtta aggtttgctt agacttactt gctccctaaa aagatgttaa
     6241 aattgacaaa tatcaattac ggcttgagca gacctatgat cccggaaaag cgaattatac
     6301 ggcgcattca gtctggcggt tgtgctatcc attgccagga ttgcagcatc agccagcttt
     6361 gcatcccgtt cacactcaac gaacatgagc ttgatcagct tgataatatc attgagcgga
     6421 agaagcctat tcagaaaggc cagacgctgt ttaaggctgg tgatgaactt aaatcgcttt
     6481 atgccatccg ctccggtacg attaaaagtt ataccatcac tgagcaaggc gacgagcaaa
     6541 tcactggttt ccatttagca ggcgacctgg tgggatttga cgccatcggc agcggccatc
     6601 acccgagctt cgcgcaggcg ctggaaacct cgatggtatg tgaaatcccg ttcgaaacgc
     6661 tggacgattt gtccggtaaa atgccgaatc tgcgtcagca gatgatgcgt ctgatgagcg
     6721 gtgaaatcaa aggcgatcag gacatgatcc tgctgttgtc aaagaaaaat gccgaggaac
     6781 gtctggctgc attcatctac aacctgtccc gtcgttttgc ccaacgcggc ttctcccctc
     6841 gtgaattccg cctgacgatg actcgtggcg atatcggtaa ctatctgggc ctgacggtag
     6901 aaaccatcag ccgtctgctg ggtcgcttcc agaaaaacgg tatgctggca gtcaaaggta
     6961 aatacatcac catcgaaaat aacgatgcgc tggcccagct tgctggtcat acgcgtaacg
     7021 ttgcctgatt tttccgcata actcactatc cttctgtcat atcattaaat ttttctgatt
     7081 tattgatctg gcagaaggtt catcactgtt tcattcacca gatatgggtt aatcttttaa
     7141 ttacaaactg cgttgacagt tgttgtaagg agaccctgta tggctatgta tcagaacatg
     7201 ctcgttgtta tcgatcctaa ccaggacgac caaccagcat tgcggcgagc tgtttattta
     7261 catcaacgga ttggtggcaa aattaaagcc tttttgccga tctatgactt ctcatacgaa
     7321 atgaccaccc tgctctcccc ggacgaacgt accgctatgc gtcagggcgt catcagccag
     7381 cgtacagcct ggatccacga gcaggcaaaa tattatctca atgctggcgt tcccattgaa
     7441 attaaagtgg tctggcataa ccgtcctttc gaagccatca ttcaggaagt gatcagcggc
     7501 gggcacgatt tggtgctaaa aatggcgcac caacatgacc gtctggaagc ggtgattttt
     7561 acgccaacgg actggcacct gttacgcaaa tgcccaagcc cggtgtggat ggtgaaagac
     7621 cagccgtggc cggaaggagg taaggcgctg gtggcggtga atctcgccag tgaagagccg
     7681 tatcataatg cgctcaatga aaaactggtc aaagagacga tcgaactggc agaacaagtc
     7741 aaccataccg aagttcatct ggttggcgct tatccggtaa cgccaattaa tatcgcgatt
     7801 gaactgccgg aatttgatcc gagcgtttat aacgatgcca ttcgtgggca acatttgctg
     7861 gcaatgaaag ccctgcggca gaaattcggc attaatgaaa acatgacgca cgtagaaaaa
     7921 ggtctgccag aagaggtgat tcctgatttg gcggagcact tacaggcggg gattgtggtt
     7981 ctgggcacgg taggacgcac cggtatttca gcagcattcc tcggcaacac ggcggaacag
     8041 gtgattgatc atcttcgctg cgatctgttg gtcattaaac ctgaccagta tcagacaccc
     8101 gttgaactgg atgacgaaga agacgattaa cgactcctca ataactgaca aggcccgagt
     8161 tatcgggcct attttttact tactgctctt cgccgccaag gaaataaatt cccaacggga
     8221 ttgccagcaa cacagtaaag accaggctat aaacaaaaat catcgatgac tgaatcacat
     8281 acattgatgt cgtccactcc gacagcggaa tattgtactg ttctataacg ccgccgatag
     8341 tcgcgcgagt cacaaccgac gccgcaataa tgaaaaccgc caacaaacca aagagaaatt
     8401 tcttcccttt cgctgacttc agtttcgcca taatcatgat gtaatccttg tgcagacaaa
     8461 tttcttgtta cctaaacgta acaataccat gacatttcac cattgtctgc tctgaaaacc
     8521 tcacctctta gctggattta caatcaacgc attgattagt tagctaatgt tgctacttcc
     8581 ttccctgact attgatgatc tgaatgaatt gtcgcggtaa aatagccccg ccaggggttt
     8641 ccggcatgac gacaattatc tggaggagcc tgataaaaca atgatcgctg aactgtttac
     8701 aaataatgcg cttaatctgg tcattatttt cggtagctgc gcagcattga ttctgatgag
     8761 cttttggttt cgccgcggaa atcgtaaaag aaaaggcttt ttattccatg cggtgcaatt
     8821 tttaatctac accatagtta tcagtgctgt tggtggcatt attaattatg ttatagaaaa
     8881 ctataaactc aaatttatca ctccaggcgt tatcgatttt atctgtacgt ccctgattgc
     8941 ggttattctg acgattaagc tatttttgct gattaatcag tttgaaaaac agcagattaa
     9001 aaagggtcgc gatatcacca gtgcgcggat tatgtcgcgc attatcaaaa tcaccattat
     9061 tgtggtactt gttctgcttt atggcgaaca tttcggcatg agcctttctg gcttgctgac
     9121 ctttggtggt attggtggtc tggctgtcgg tatggctggc aaagatattc tgagtaattt
     9181 cttttccggg atcatgctct atttcgaccg tcctttcagt attggcgact ggatccgttc
     9241 accggacaga aatatcgaag gtacagtagc ggaaattggc tggcgaatta ccaaaattac
     9301 gacctttgat aatcgtccat tgtacgtacc gaactcgctg ttttcatcga tcagcgtaga
     9361 aaacccagga cgaatgacca accgccgcat taccacgacc attggtttac gttatgagga
     9421 tgcggcaaaa gtgggcgtta tcgtcgaagc cgtacgtgag atgctgaaaa atcacccggc
     9481 catcgaccag cgacaaacct tactggttta tttcaaccag tttgctgact cgtcattgaa
     9541 tattatggtt tattgcttta ccaaaaccac ggtatgggct gagtggcttg ctgcacagca
     9601 agacgtttat ttgaagatta tcgatattgt acagtcacac ggcgcggact ttgccttccc
     9661 cagccagacg ctgtatatgg ataacattac accgccggaa cagggtcgct aaatcagaca
     9721 tcactggcgt tatccacaag ggtaacgcca gttcccatca catcaatgct tcacaatata
     9781 catagtccga ctgtacgcca catcctcagg gttattaatg ggataacctt tcagccacgg
     9841 tttgattaat cgtccattgg tatattgata aattggtgca atcggtgctt gctccatgag
     9901 gattttttct gccgcgttgt aatcggcatt acgcgcttta acggtatttt ccgtcgatgc
     9961 ctgggccaga actttgtcgt atgccggatt gttaaagcgt gaaatatttc ctgaatgcgt
    10021 tgacgttaat aatgtcagga aagtggaggg ttcattataa tcccccaccc atgaggcgcg
    10081 gataacatca aaattgccgg tgttacggct atcgatatag gttttccatt cctgattttg
    10141 caatttaaca tctacgccaa ggttcttttt ccacatcgat gctacagcaa tcgcaatttt
    10201 ttgatggttt tctgaagtgt tatacaaaag cgtcagcttc agcggttttt gcggaccata
    10261 accagctgcg ctcaacaaag tttttgcctg cgcattcagt tcttcctgac tcatttgttc
    10321 aaacggcgaa ggttccggcg taaatccggc ggtaacatct ggtgtaaaat gccacgctgg
    10381 cttttcgccc gtccctaata ctttttcggt catcaggcgg cgatctatcg tcatacttaa
    10441 tgccagacga acgcgctgat ctgccgtcgg ccctttttgc gtgttaaacg cataataata
    10501 ggtcccgagc tgcggcggcg tataaacctg ccccggaata tccttcaaca gcttctgata
    10561 catatttttc gggaaggatt cggtgatatc aatatccccc gcgaggtaac gcttagtggc
    10621 tgcggattcc tgattaattg gcaggaaggt cactttttgc agtaccgttt tggcgttatc
    10681 ccaataatgg gtattcggta ccacgaccag tttttcattg actacgcgct ctttaagaac
    10741 ataagcgcca ttgccgatca gatttccggg tttcgtccac tctttaccgc tttctacgtt
    10801 ggctttttgc accgggaaga aggcaaagtt agcggttaaa ttcacaaacc acggcaacgg
    10861 tttatcaagc tgaattttca aagtatggac atcaactgcg gtgacgccaa gctggtcagg
    10921 tgtagcttta ccatcaataa tcgcctgtgc gttgttgatt cccgccagcg cggcaaacca
    10981 tgcaaatggc gatagcgttt tcgggtccac cagacgctgc cagctgtaga caaaatcttg
    11041 cgccgttacc ggtgtgccat ccgcccattt tgcgttatcg cgcagggtaa aagtccagat
    11101 acggttgtca ttacttttcc actgagtcgc aacgccgggg acaatctccc ctttctcgtt
    11161 ctgattcacc agtccttcaa acagatcgcg aatgacctga atctctggca ggcccacggc
    11221 tttagcggga tccagcgacg caggctcatc tttaatgtgg cgcaccagct cctgcttctc
    11281 tgccagtact gtgccgctcg gaacttctgc agcatacgaa agagaaatgc tgctgaccaa
    11341 cagcgcacaa cacgtgactg aaacagagtg cttcataagt ttccctctaa ccgacattaa
    11401 aattgatgcg taattatttg tttcgcctta aagaaatgca aacaacttcc atcagaaagt
    11461 gacacaggaa gcataaaatg ttttatgtat caatatatta aatcagattt agtgtaaata
    11521 atagacgcca aaatgcggca aagcacatag aacaccgaac actacgttcg ggcttcacac
    11581 gttcaaacca gttttcggta tcataatcag catctgtcgc cattgtacga agaataaaaa
    11641 ataaccaaaa cgttgaaagg aactgagaat acgttaagag aagaattaaa ttaatgtatc
    11701 agcaagtagt gctaaacagc acctttatac ttcagcctat ctataaataa tgagaatgcc
    11761 gagccagcat gtcgacggtg tggatagtac aggtgataac cgggtaaatc tggtgtgaat
    11821 ttatccaaaa cacgtatcag ttttttttct ttaatcgccc gctcaacctg atcataaggt
    11881 agatacgcca atccatgccc atcaattgca gcatcaatga tcaggtctat cgtattcagt
    11941 aaaagctgac cttccatgcg aacacgaact tcacgtccac cgcgtattaa tctccagcga
    12001 tttgctgtac ccgatgtggg aagatacaaa ttaattgcct gatgatctat taattgtgac
    12061 actgacgttg gaacacttcg gcgagaaaaa taatccggtg agccaacaat agccattggt
    12121 atatctggcc cgattcgaat ggcgatcata tctttatcca tctccccacc cagacggacg
    12181 cctgcatcaa aacgttcaga aacgacatca gtcaaaccat aatcaatgat aagctgaata
    12241 tcaatttcag ggtgtgattt caggaatgtg cgcatggctg gtaacaatat cgtttttgct
    12301 gcatgttcta cagtagtaat acgtattgtc ccggatgggc ggttctgcag atcgctcagg
    12361 gatgccaggg cagaatctat gtcatgcagc atagggccaa gaacagacaa aagatgctcg
    12421 cccgcttcag tgggtacaac actgcgcgtg gttcgcgtca gaagccgcaa tcccaatcgt
    12481 tcttctatac ggcgcactat ctggcttaaa gctgactgcg ccatgctcag gcgggctgct
    12541 gcgcgagtga agctacgctc ctctgcaacg acgacaaacg ccatcagatc agcgatctct
    12601 tcacgcttca ttcagtatct ccatacaatc tattaatcat ttaaatgata ggtctaatca
    12661 tgatttaggg tctaatcaac ttaatgatca atatctatag tgatatcacc aaccgttaaa
    12721 acgaaccaac aactccatga ggaacggaat gatgaataat aaagtcagct tcactaacag
    12781 caataatcca accatctctt tgtctgcagt gatctatttt cctccaaaat ttgatgaaac
    12841 ccgtcaatat ccggcaattg tggtatctca cccaggtggg ggcgttaaag aacaaacggc
    12901 cgggacctat gccgaaaaac tggcggaaaa gggatttgtc actattgctt atgacgcatc
    12961 ttatcagggt gaaagtggcg gcgagccgcg tcagttagaa aacccataca ttcgtactga
    13021 agacgtcagt gccgtgattg attacctgac aacgctttct tatgtcgaca atacccgaat
    13081 tggtgcgatg ggaatttgtg ccggtgcagg atatacagcc aatgctgcaa ttcaggatcg
    13141 ccgcatcaag gccatcggta ccgttagtgc ggtcaatatt ggttcgatgt tccgtaatgg
    13201 ttgggaaaac aatgtgaaat ctattgatgc cctgccatac gttgaagcgg gttcaaacgc
    13261 cagaaccagc gatattagca gcggagaata cgccattatg ccactggcac caatgaagga
    13321 gtccgatgcg ccgaacgaag aactgcgtca ggcctgggag tattaccaca cccctcgcgc
    13381 gcagtatcca acagcaccgg gttacgctac tttgcgcagc cttaaccaga ttattaccta
    13441 tgatgcttac catatggcgg aagtgtacct gactcagccg acgcagattg tggcgggcag
    13501 ccaggcaggg agcaaatgga tgagtgacga cctgtacgat cgggcatcaa gccaggataa
    13561 acgctatcac attgttgaag gcgcaaacca tatggatctt tatgacggca aagcctatgt
    13621 ggctgaagca atttcagtat tagctccgtt ctttgaggaa actctgtaat gaaacatatt
    13681 caaatccgca atagtgatat ggcctggcat attgctgcca atattcagtt tccaccaaat
    13741 tttgatgagt caaaacagta tccggcgatt atcagcgtcc atccttttgg cagttgtaag
    13801 gagcagacgt ctggcaatat ctatggcaag gcattggctg aaaaaggcta tgtcgttctc
    13861 gcttatgacg ccagtttcca gggagaatct ggtggggagc ctcgttggat agaagatccg
    13921 acacagcgcg tagaggatat tagccgtgtt atcgattatg cagtaactct gccatatgtt
    13981 gatgctgaac gcattggcgt gctgggtgtg tggggggggg ggtggctatg ccattaatgc
    14041 cgcgctgaca gaaaaacgaa ttaaagctgt agtgagtatt acgggtgtta atatcgggcg
    14101 tctttttcgg gaaggtttca gcaattacga tccgattggc gcattgaatg caatggcgtc
    14161 gcaacgaacc aaagaggctc gtggtggaga attacagatc aacgaattac ttcccgccag
    14221 cctggatgca gcaaaagccc atggactgac cgaacgcgat gtttatgaag caactgacta
    14281 ctataaaaca ccacgcgggc aacaaccggg aggtgcgaca aaaatgttgt tctcacatgc
    14341 acagaaaacg ctggcctggg atgccttcgc atttactgaa gtgttactga ctcagccggt
    14401 gatggtagta gttggtgaaa aagtcggtgc cttcggtgct tatcgtgatg gactggaagt
    14461 ctatggccgg gcgatggttt cgcaggatcg tcagttagtt tcattacccg atttctcaca
    14521 ttatgaactg tatgacaaac cggaagcagt tcaggaggcg ctggaaaaag ttattccttt
    14581 tttcaacacc catctgtgat aagtttcatg agaaatatta tgaaaaatgt actcattctt
    14641 ggtgctggcg gtcagatagc ccgccatgtg attaatcaac tggcagataa acaaaccatt
    14701 aaacaaactt tatttgctcg tcagccagca aaaatacaca aaccctatcc aactaatagc
    14761 cagattatta tgggggatgt actgaatcat gcagcactga agcaggccat gcagggacag
    14821 gatattgtgt atgccaacct tacgggggaa gatttagata ttcaggcaaa ttcagtgatc
    14881 gctgcgatga aagcctgtga tgttaaacgt ctgatctttg ttctttccct cggcatttat
    14941 gatgaagttc caggaaaatt tggcgaatgg aataatgcag ttattggtga gcccctaaaa
    15001 ccattccgca gggctgcgga tgcaattgag gcttctggac tggaatatac aattcttcgc
    15061 cctgcctggc tgacggatga agatattatt gattatgagc ttacctcacg caatgaacca
    15121 ttcaaaggta cgatcgtatc acctaagagt gtcgcagcat taattacaga tatcatcgat
    15181 aaacctgaga aacacattgg tgagaatata ggcatcaatc aacccgggac tgatggcgac
    15241 aagccattct tcatgtaaat caattatata accaccatcg tactgtacta tgtgcagtac
    15301 gatttattaa acttattaag gtgaactgat caatcagatg cgattaacat gtgtagaacc
    15361 agtaagaata aaatacgcca tgtatataac attacgacaa ttctaatatc tgcacatcaa
    15421 tgaaaataca aacaacttcc ggcaaaaaag tgatacaaga tcgatgacat agcgttgaag
    15481 aaaaggattt gataatccgc aaaatttgca caacactgcc tgtaatactt cctgaaccag
    15541 agacgatcat gaccgtaacc cgcccacgcg ccgaacgcgg cgcatttccg cccggaacag
    15601 aacattacgg acgttcatta ttgggcgcgc cgttaatctg gtttccggcc cctgcagcca
    15661 gtcgcgaaag tggtttgatt ctggctggca ctcacggtga tgaaaactct tcggtcgtca
    15721 cgctctcctg tgctctgcgg actttgacac cttctttgcg ccgccatcat gtggtgctgt
    15781 gtgtgaatcc tgacggctgc cagctggggt tacgggccaa tgctaatggt gtggatttaa
    15841 acagaaactt tccggcggcg aactggaagg aaggtgaaac ggtttatcgc tggaacagcg
    15901 ccgctgaaga acgtgatgtc gtattactga caggtgacaa acctggctcc gaacctgaaa
    15961 cccaggcgct gtgccagctt atacaccgca ttcaacctgc ctgggtggtc tcattccacg
    16021 atccactggc ctgtattgaa gatcccagac acagcgaatt aggcgaatgg ctggctcagg
    16081 cgtttgaatt gcctctggta accagcgtcg gttatgaaac gcctggctct ttcggcagtt
    16141 ggtgtgcaga tctgaacttg cattgcatca ccgcagagtt tccgcccatc tcctccgacg
    16201 aagccagcga aaaatatctc tttgcgatgg caaacttgct gcgctggcat cctaaagatg
    16261 caattcgccc gtcgtgaact gaagcgccgg ttccacatct accgccagcc aggtcggtcc
    16321 gtcaagatcg gcgaaactga cctgcggcac cagcggtaaa gcggcgctaa tggcccgaga
    16381 ggtacacaac atgcagccca gcatcagact gaaaccttgt gcacgcgctt cagtcgccag
    16441 cgccagcgct tccgtcagac ccccggtttt atcgagctta atattaacca tctcatagcg
    16501 ccctttcagc gccttcaaat tgctacgagt atgacaactt tcatcagcac aaatcggcaa
    16561 cggatgaata aaattctcca gcgccgcatc gtcctgcgca ggaagcggtt gttcaagcat
    16621 cgcaacgcct aaatccgcca atagctggca acgtgccgcc aacccttctg catgccagga
    16681 ttcatttgca tcaacgatca gcgtggtatc gggcacagct gtgcgaattg ccaccatccg
    16741 ctcactgata agatggttat ccagcttcac tttcagtaat ttcgcgccgg cctgccagag
    16801 tgttgatgca ctattggcca tctgatcagg cgtaccgatg actaccgtct gtgcagtgat
    16861 aactgtctcg ggaagcgtta tgccgatcaa atcagccagc gattgctgct gttttcgcgc
    16921 ggccagatcc cacaatgcac aatccagcgc attacgtgct gcgccggcag ggagaatttt
    16981 ttgcaactcc tcccgtgtca gccctttctc taattgcggc acgacgctca taatttgcgc
    17041 cattaccgag gcatcacttt ctccataacg cggatacggc gtgcattcgc cggtgccttt
    17101 aataccctct tcttccagtt caaccaccac cacgcgcgct tcactgcgac ttccccgggc
    17161 aatcacaaac ggggtatgta agggccaggc ttcctcaaat accttaacgg ttctcatctt
    17221 tactccttgg tgtcgcggtg gtctgcggat taagcgcaaa tagcgtttgc tgtgttattg
    17281 acagttagca taaactaggt gtgacgttaa ctatatgtaa acaggaaaga taatcatgtc
    17341 acaaaccgtt catttccagg gcaacccggt tacagtcgcc aattccatcc cgcaggcggg
    17401 tagcaaagcg cagactttta ctctcgtggc aaaagatctg tctgacgtca ccctcggtca
    17461 gtttgcgggt aaacgcaaag tgctgaacat tttcccgagt attgataccg gtgtttgcgc
    17521 cgcatcagta cgtaagttta accaactggc aaccgagatc gacaacaccg ttgtgctgtg
    17581 tatctctgcc gatctgccgt tcgcccagtc tcgtttctgc ggcgcagaag gtctgaacaa
    17641 cgttatcacc ctctccactt tccgtaacgc tgaatttctg caagcctacg gtgtggcaat
    17701 tgctgatggc ccactgaaag gtctggcagc gcgtgccgtt gtggttattg atgaaaatga
    17761 caatgtgatt ttcagccagc tggtggatga aatcaccacc gagccggatt acgaagcagc
    17821 tctggctgta ctgaaagcat aagttaatat gcctgatggt gttgcaccat caggcatatt
    17881 cgcgcttact cttcgttctt cttctggctt aagccatatt cccgcaactt attggcaatc
    17941 gcggtatgtg aaacgccgag acgttttgcc agtttgcgcg tgctgggata attgcgataa
    18001 agctgggtta ataccgagcg ttcaaaacgg ctggtgattt cgtccagcga accttccatc
    18061 gcatcttcgc ccacggctac cgttgcggcg tcataatccg gcaacaaaat atcctgtgga
    18121 cgcagctcat aaccgtccag ttgtgtcagt gcgcgataga tagcgttctt taactgccgc
    18181 acatttcccg gccacgcata acgcgtaagt acagtattca ggtcagcggc cagtttcgga
    18241 cgcggcacgc cctgctcgtc ggcaaagcgg gcgacgaaca gctcagttaa cggcatgatg
    18301 tcctgcggac agtcacgtag cggcggcagg ttgagcgtca acacgttcag acgataatag
    18361 agatcttcac ggaacatgcc tttttgcacc agttcgacca gattcttctg cgtagcgcaa
    18421 atcacccgca catcgacatg cacctcatgg tcttcgccaa cccgacggaa agtgccatca
    18481 ttaaggaaac gcagtaattt cgcctgcatc cgtggtgaca tctcacctat ttcatccaac
    18541 agcaccgaac caccgttcgc ctgctcaaag aatcctttct tcccttccgg agcatgacca
    18601 aacagctcac tctcgaccgc atcttccggt atagacgcac agttcagcgc caggtaaggt
    18661 ttgctcgctc ttgggcttgc ctgatggcag gcgtaggcaa aaagatcttt acctgtacct
    18721 gtgtcacccg taatcagcag cggcgcgctt agcatcgcca gtttctgcgc ctgttcgaca
    18781 acatgcttca ttttcgggct gacagcgaca atttgactga aggcgctgac gtcctgggcg
    18841 gcgacatttt gcaactgacg gcccatacga atcgttgatc gcaacatcac caccgcacct
    18901 gtcaggacgt gttgatcatt ttcatcctga agataaacag gcgtaatctc catcaggaag
    18961 ttctgtccat taataacaac atgctcgtta tgggaatctt gcggttcgct ttccagccaa
    19021 cgtaaaaaat taaagccgtt aatcaattgt gcggcggtat ggttgcgcag tcgatccaat
    19081 ttttgcccaa aaagctgaca gctcgccggg ttcgccatat ccactttgct tttcatatcg
    19141 acagagagca caggttcagg caacgcctcc agtaacgcgc tcaacgccag atgctcacgt
    19201 tcggaaggca tccacgggac agtacgcaca tcggtaacac ctgcaatacg gcgtatttcg
    19261 gccatcagac tgctgaaact ctcaaactcc agttcagcaa aattgaggta gattcgccca
    19321 atgggatcaa tctcaatacc gcgtaaatca atgcctctta gcacgagtag atcgagtaat
    19381 tcgcgggtca gaccgagtcg gtcttcacaa aagacttcca gacgcatggg aaccttcacc
    19441 tgaaaaaaga acaattaata tgatgatatg acactatttg aatagcagga aggtttctgt
    19501 caacaatcat tgacacaaag acggaaaaac gggcacttcg gcgtaaagat atccggtcag
    19561 cacccgatat tgcattattt ttcgctgggc gttttgcctt tttgcagcgt ttctttcacc
    19621 tgaccgataa gctgacgacg gaaatccccg aggcgaggtt tgtcatcgtc aatccacggc
    19681 agcgggcggc aaagctccat agctttaatc cccagtcgtg ccgtcagaag tcctgcacca
    19741 atcccctgag ctgcgcgggt agacaaacga gcagcgagat cttgcgacat ccagtccatt
    19801 cccacttcgc gcaccaattc gctggctccg gcgaaagcga tattcagcaa taccagcttg
    19861 aacaggcgca aacggctgta atatcccagt tcaatgccat acagcgtggc gatgcgatta
    19921 atcaaacgca gattgcgcca ggcgataaat gccatatcca ccagcgccag cgggctgacc
    19981 gcaatcatca acgttgattc agctgctgag cggctgattt cgcgccgcgc ctgggcatct
    20041 aaaaccggct ggaccagatg agcatacagg ctgaccactt cacgatcgtt ctgcgtttca
    20101 tggattgagg cataccagcg ttgcagcgct ggatgagact gatcaatacc cgcctgctgc
    20161 gccagttttt cgcaaaatgc gcggcctttg cccgtgccgt ggctgtgcaa caaatcgcgc
    20221 gcttcgtcgc gttcatgggc gcgctgtcgc aagcgccata agcgccgcca ctctgttacc
    20281 acagaaccta cgccagcgcc gataatcaat gccccagcag cacatccacc cagcgccacc
    20341 cagtcctgag tttgccaggc attcattgtc cactgtacac cctgcccgac aacgcttgcg
    20401 ccaaacagag ccagcccgcc catcaccatt ttgcgccaca ggctgcgttt cggacgtaac
    20461 gctgcgtcca ttaccgcttc gacttgcccc tcttcttcct gcgcttcgtc gagcgtggcc
    20521 ggggcaaaat tttgcgcctg attttcgtca aaggtctgct gcgccctgaa ttttggattc
    20581 tgttcgacct ccagcggacc gtcgaaatca atacgtggtt ttaacggttc ggtcatcgca
    20641 atttatctcc tattaaaaat tccagcgcgg catcaagacg aatatgcggc agcggtttgt
    20701 cgacatccat cacctgcggg cgaaacgctt caaactggaa cccttgctta tcccagaacg
    20761 cctgaccagg caaacgtgcg ggaacttcgc caggataaac agtgagcggt gcgccatcgc
    20821 taagtcgatt accgcgcagc gccgggattt tctcaccgtt aacatcaata atgccactgg
    20881 tggtcgcctg aactgacgcc agccccaggc aatccatgct gatcccttcg aacgccgcac
    20941 tttgccaggc atcctgaatt agttgttgca gcaatgaaac catattggcg tgctgatcga
    21001 tggtcacatg gtccgcttta gtggcagcaa acaatagctt atcgataacc ggcgaaaaca
    21061 aacgcctgaa cagggtacgc tgcccatagt ggaaactttg catcagctgc gtcagcgcca
    21121 gacgcatatc attaaatgcc tgtggcccac tgttgagagg ttgcaggcaa tccaccagca
    21181 caatctggcg gtcaaagcgc agaaaatgat tcttatagaa ccccttcacc accttctcgc
    21241 agtaataatt aaaccgctcg cgcagcattc cggcattggt gtgcttatcg gcctgcgcca
    21301 gtttggactc gccccaggca tcgacatccg gccacgggaa gaattgcagc gcgggcgcac
    21361 ctgccatatc ccccggcaag acaaagcgcc caggctgaat aaagtgcagc ccctgctgtt
    21421 tacagtggtg gagataatcg gtccacgcgg cggcaatgtc tgccagccgg ttttcgtcgg
    21481 caggcgctag cgggtccagc ccttcgcaca tcattcgcca tttcgccgac cattcgccgc
    21541 gctgaccatt gagtaagccc gtcatctggc gcgaccagct taaatagtcc tgcgccagca
    21601 tcggcaggtc gagcaaccat tcgccagggt aatccacaat ctccagatac agcgtggagg
    21661 tatccttaaa gtggcgcagc agcgaatcgt tcgatttata gcgtagtgcc aggcgaattt
    21721 cgctgacgcc gcgcgttggc gtcggccagg cgggaggatc gccatacagc tgcgccaacc
    21781 cttcgtcgta ggtaaaacgc ggaatgccaa agtcacgctg aggaatgcgt tttacgccca
    21841 gcagacgctc ttcacgcacc gcacttaata gcggcaaacg tgctccggca tgaatattga
    21901 gcaactgatt gaccatcgca gtgataaacg ccgttttgcc gctgcggcta agtccggtta
    21961 cggcgaggcg cagatgtctg tcgacacccc gattcaccag cgcattaagt tcatttttaa
    22021 gtcgcttcat tgctgtcctg tgctgctctg aagaccagaa aaaaatcatt cataaggata
    22081 tgaatgtggg ggccaggcca ttgattatca actacccgtg ttgtcatcga cgggcgaact
    22141 ccataagatg tgcagcattc acgttcggac ggagttcctg acctatgtcc cctactattt
    22201 atgatattgc cagggttgca ggcgtatcaa aatccaccgt atcacgcgtg ctgaataagc
    22261 aaaccaatat ctccccggaa gcgcgcgaaa aagtgttacg ggccattgaa gaattacagt
    22321 atcaaccaaa caagctggcc cgcgcgctga cctcttcggg ttttgatgcc attatggtga
    22381 tttctacccg ttcgaccaaa actacggcgg gtaatccgtt tttctctgaa gttttgcatg
    22441 ccatcaccgc caaagctgaa gaagaaggtt tcgacgtgat attgcagacg tcgcacaccc
    22501 cggcagaaga cttacaaaaa tgtgaaagca aaattaagca gaaaatgatt aaaggcatta
    22561 ttatgctaag ttcgccagcg gatgagtcat tttttgccca acttgataaa tacgatattc
    22621 ctgtggtggt gattggcaaa gttgaaggtc aatatgccca tgtttattct gtcgataccg
    22681 ataattttgg tgacagcatt gcgttgaccg atgcgttaat tgaaagtgga catcaaaata
    22741 ttgcctgtct gcatgcaccg cttgatgtcc atgtttcagt ggatcgggta aatggttata
    22801 agcagagtct ggaagatcat aatattgcag tgcgtgatga atggattgtt gatggcggtt
    22861 atacccatga aacagcactg caagcagcac ggcaattatt aagtcagtca ccgttgcctg
    22921 aggcagtgtt tgccactgac agcctgaaat taatgagcat ttatcgtgcg gcggcagaga
    22981 aaaacattgc tattccgcag cagttagcgg tggtgggtta tagcaatgaa acgctgtcat
    23041 ttattttaac gcctgcaccg ggcggcatcg atgttccgac gcaggagtta ggtcgacaaa
    23101 gctgtgagtt attattccgc ttaattgccg gaaaaccgtc accacaaaat attaccgttg
    23161 ccacgcatat gacgctgaaa taaataccag cgaccttgcc tgaatgccgg atgcgatgct
    23221 ggcgcatctt atccggccta catttataga tcggataaag tactcgcgcc gcatccggca
    23281 atttagccca ttatcagaac gagtaattta cgccgacacc tgcataatgg aatttatcgc
    23341 tgtcgccttc gtcgtgatcc tgccactcaa acgcgtattc cagcgaaatg gaaagaccat
    23401 tctggaaatc ataaccgtaa aataaaccaa cacggttaaa atcatggcct tcacgttcaa
    23461 tatcatcctg ccagtcccag ttactccagc gatccagccc aatgcgcgta tacggcgtca
    23521 ccgagtggtt gccgagcgtc agcggcaaat aggcgcgaat ttcttgcgtg gaaaactcac
    23581 cgttattacg gctgtcgtcc atattgaagc cgcgctcgag ataatagttc actcgcaagg
    23641 caaccgtttc gttgaaggta tattgcagac ctgtttccgt ttcgacacgg gtatcagcgt
    23701 aaccggtcgt gttcaaatcg ttggcaaatt tatacatcga caaccaaccg ttgaaacgta
    23761 aatcgtcagt cagtttcaca tcccagtctg gcgcgatttt ccagcgctgc atattcgccg
    23821 tgtctttacc cggttcatca acgtagtgat aaccataatt acggaaaccg ccggtcaggc
    23881 cgaaactgaa atcatcgctt tcgaggaact gataatgtac ctccagctcc gggcgatcaa
    23941 accacgttcc acgtttaccc gcgctataat ctaccggccc ttcctgataa taggccagag
    24001 aaattctcca cggcccgttg gcggcattaa aatagacaga aggctccgcc agcccatcca
    24061 tatcttcgcc ataaccctcg acgttttcta tttcgtacat cgcgccgata ttaaagtgcc
    24121 agtcgttctt ttcctcggcc tgtgcgcagg ccattcccgc acacatcacc agtgcggtac
    24181 agggtaataa ctttttcatt attgttcctt gtattatcct gggggaatgc cttccccctg
    24241 tatttagcga attgctattt ccgtttctgc atcaaagaaa tgacatttag tcatatcaaa
    24301 atgaatagtg atattttctc ctgcatgata atcatttaac gcaccagcac ggaccactaa
    24361 ctcgtgcccc ccaaccgtgg tgtagagcat aaattccgca ccggttaatt ctaccacgct
    24421 aattttggcg gaaatattat tttcctcttg cgcgtccgga tgaatatctt ccgggcgcat
    24481 tcccatcacg atgggcttat gcaaactttc ctgtgttttc agaaccgcta atttctcttc
    24541 gggaatggtt aatttaagcg tttccgtaac gaatttatcg ccatcgatcg tgccgcgaat
    24601 aaaattcatc gctggtgatc caataaatcc ggcaacaaac atattcgcgg gttggttata
    24661 aacggttttc ggcgcaccca cttgctgaac aatcccgtct ttcataatca caatccgcgt
    24721 cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tgttcagttt
    24781 ctgatgcagc ttgctgatct ctgcgcgcat ttgtacgcgc agcttggcat caaggttaga
    24841 gagcggttca tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac
    24901 acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgc ttcaggtact cacgcaggcc
    24961 gagaatttgc gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat
    25021 cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg
    25081 gaacaccatt gcgatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat
    25141 caacagatcg ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgattt
    25201 accgcagccc gacgggccga caaatacgat gaactcttta tcggcaattt ccaggttgaa
    25261 gtccttcacc acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg
    25321 agccatgttg attcctttgc tatacgtttt gccagaaggc cgataaccgc ggccaggtca
    25381 gggagtccgt tgagggcaac aataattgcg ccccggttaa gcctgcgccg atccccaccg
    25441 agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac
    25501 atgcctgcgg cggcacgccc agcccggcac aggcagcgag aaagatttcc ggatccggtt
    25561 tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa aaactcgcgc agctccagcg
    25621 ccgctaaaat cgtcggcgca ttcagggaga cagaagccag ccctaccggg atctgctgtg
    25681 cgcggagctc tgccagcaaa ttgcgaatac cgggcagcac agcattaacc gtcagctcgc
    25741 gcagtgagtg gacgtaaagc agatttttgc gatacgccag ttgcgtccgc tcctgcgggt
    25801 taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga
    25861 tcccttttag ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct
    25921 gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa
    25981 ttaccccttg cagtttcatt catcctcctg atgcgtggta gcggtcccat tgaagggcaa
    26041 aataaaatca cccaaacaga aaacagattc ttcagccacg gatataagct gcccgttcaa
    26101 acgcagtgaa acgggcgcag aagttcgaat cgcaatacgc tgcgcgtcga gcgtgacctg
    26161 taattcgcag ccctgccaga acagagggaa agacaactgt tgccactgct ccggtaacgc
    26221 cggattgaga tgtaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc
    26281 cagccagata gcgccagttg cggcagcgtg gataccatca tcacaactat gcggatcagc
    26341 accaagatcg atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca
    26401 gcgtgcggca acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg
    26461 ttcataaaat tgcagattgg caagacacga taccgctgag aactgctccg gcagcatgta
    26521 attgagcatc accacatcag cttgtttgag gatctgcatc tcgttcactt ctgcgcgtga
    26581 ataatccagc agaatggttt gcttccccgc cgccgctttg tatttagcca gattaatcgc
    26641 cggcttagcc ataaacgaat catcctgcgg caaaacgcca tcgggctgcg tttctggcat
    26701 ccatagctct ttgaggaaca tttcggcgcg atggataaac gcatcgtcgc tacagccgaa
    26761 ctggcgggca atattcagcg cctgttgaac gttgtagcgg gccatatagc tggtgaaggc
    26821 gttattattg acatgttcgg tatattcgtc tggcccaata acatcatgaa tttccagacg
    26881 gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat
    26941 gccttcatga gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca
    27001 ggcgatatcg gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc
    27061 ggtacgaatg ttaatggcgg caaattctgg cgtctcttct tcgccgctgc gcgcgctttc
    27121 ccacggaaat agcgcgccct gccagccgct gcgccgtgct ttctcctgcg cgcctggcaa
    27181 gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatggaa
    27241 cggcagcaaa aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa
    27301 gccttttgcc gcaatactgc tgcgctcgtc gtgagccggc gtcatgatgc gcagatgata
    27361 aagcgcataa tctaacgctt gctgatcgtg cgcatcgccg ccatttaccg tgatacgacg
    27421 tttctgccac cattgacgcc agttttctgt tgatgctgca agaagttggt cgtaactctg
    27481 ctgcgcgcat atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg
    27541 cctgtcatcc cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg
    27601 aagctgcgca ctggtatgtt gcagcaaacg gcgctctttg gcggtatagc attgctgcac
    27661 atcaccgctc accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt
    27721 gtagatcccc tgcatcagat gctgaccaaa cacccgcacc tgggtttcgt cgagatgttg
    27781 gcgaccgtgg ttggtttgcg tggcgtcgat acctgttgaa atcagcactg acgcgtcggc
    27841 gtccagtggc gtaatagtga tttccagcgc aatgagcggc agttggtctg ccgaaacaaa
    27901 gcgacggctg gcgatggtgt aacctgcgcc gttgctggta cgccagacaa cgttgcggcg
    27961 taattcgcca ctggcaaagt caagctcacg ctgccaggct tcgtgggata acgagaaaac
    28021 ctcaccattt atggcaatct ccatccccaa gatatcaggc aggttcacca gttcgttgat
    28081 ttcacctttt cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt
    28141 gtaatcttct tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga
    28201 tgcatacttg ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt
    28261 cataattcca cacgcgtccc tgtttctgcg gattgatata acgccgccac cagttgctgg
    28321 atgatgtacc cctgctcggc gtcggctatc atcacgggct tgccctgtac gtgattgata
    28381 aaggcttcca tgctgcccaa atggcggttg tcgtctgcca tttcccgttg catcagcgtc
    28441 attaattcac cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accggcttta
    28501 tcaccacaaa agctgacgtt cataatcgac tgttcgcgga tgttgagcgc aaatgacgtt
    28561 tccagccaca gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg
    28621 ctgtaagttg ccggatccca ctcgccaaat tgaccacagc tcttttgcgt gccgatcttt
    28681 tgaaagctat gcgcattcac gcttttcacc gcaggatagc ccagcacata catcgcagca
    28741 tccagcatat gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta
    28801 aagacacccc agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc
    28861 tctcccaaaa cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga
    28921 tggtgaaagt cgtaggccag caccttgccc tgtttgcgcg cggtatcgca catttcccgc
    28981 gcctgttctg gcgtcatggc gggcggtttt tcgcacatca catggcagcc cgcttccagc
    29041 gccatcaggg tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt
    29101 ttcaccgcca gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct
    29161 gccagcgcct gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg
    29221 cgggtgcagt agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg
    29281 acccgcagtg gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac
    29341 caggtcaacg aatcccggta cgcctgggca ggatcttctg cccggatacg cccttcatac
    29401 accacataac cctgatagtt atcggcgcgc agctgttcaa acagcgcgtg gaaatcgagg
    29461 gtgccgctgc ccggctggta acgatggtta tcggcaatat gcacatgacc gagcaagtcg
    29521 cggttgtcat gcagcgcctg cgccaggtta tcttcttcga tgttcatgtg atagaaatcg
    29581 ccgataatct gcacatgttt aagattgttt tcgacgctgt aacgacgagc atcggcgagg
    29641 gtgttgatca tatgatcctg atagcggttt aacggttcga gatacaccac ggttccggta
    29701 cgcgcggcga cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc
    29761 aggctacgcg gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga
    29821 acgacgatac ctttaccccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt
    29881 aagccattaa gacgacgctc ttcgataaag tcaccaatcc agccgtcata gccgccgcag
    29941 gcggtggtca ccggtaaacc ggtttctttg atcgccgatt tgacttcttc gatgttgttg
    30001 accagcagtt tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat
    30061 ttctccagaa tattttccgg gaagaacgcc tgattctgtg tgcctatttt cattttttca
    30121 ttcctttgaa ttaaaacgtg acgcccattt tgatgctctg ttccggatgc tgatcgacat
    30181 actgcatgta gctttccggg ctgttggcaa aggtcactac cgggtcgatt aaatcttcgc
    30241 aattgagata accgttcatc agcagtttcc agcaggtttc ttcaatacgc ttacggctcc
    30301 agcgcggata atccgggttc ggttcactgc acgcgcgaga gaagacaatt ttggcgttat
    30361 tgaaatgcgc ttcgcgtccg aggttaaaac cttcagcaaa cggtttagca aacgcgacat
    30421 aggagatggt gccaccgtaa gccaggccgc gcagcgccga ctgcagcgcg tcggcgtaac
    30481 cgctggtttc gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc
    30541 ctacgtcagt gccaatggga ttaaggcaga aatccgcacc gtgacgacga gcaatatcac
    30601 aacgatgggc aataggatcg acgccaatta ccaccgacgc gccagcgcgt ttagccagtt
    30661 ggatggcaat ttgaccgatc gcgccaagcc ctaccaccac tacaaaatcc cctacgcgta
    30721 cgttggcatc gcgaacgcca ctcatggcaa actgcgccgg gtcgtagcag acagcgtttt
    30781 tccaggagct gccttccggc attttgcgca gcttgtagtt attcactgcg ttaatgatga
    30841 tcgtctcgga gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt
    30901 cgctgccgca ctcgataatg tcgccaacca ccatgttgcc aagctggaat ttgccaaact
    30961 caatgccgcg cggcgcatct gccggacgcg gggtgaacat ctgccattcg ccgttaaagt
    31021 cttcatcgat aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg
    31081 caccgaaacg cacgcggatc ttcacttcat tggctaaaat cgcccgatct tcatactcaa
    31141 ccagcgcagc aacgcgcggt gctgtggcta ctaacttttt catgtaaaac tccttgtaaa
    31201 gaaactggcc gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt
    31261 ccgaaagcgc atacataatc accaccggca atgcggtcac cagcgaggcc gccatcatcc
    31321 gtccccagat gtaatctggc gtactgaaca gcgcgttcag gccgactggt aaggtgaaat
    31381 tgctggcgct ggagaggaaa atcgacgcaa acagatagtc gttccatgcc accataaagc
    31441 agtagacgaa tacggatatc agcccggaca tcgccagggg cacggtgata cggaagataa
    31501 tttgcagacg gttgaggcca tccatcatcg ccgcttcttc gatctcatca gggatggtgt
    31561 cgaagtaact tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtaatga
    31621 tcagcgccat ctcggtgtcg taaatgccaa gtgcggtgat aattttgaac agcggcacca
    31681 ccagcaaaat gccggagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccggc
    31741 ctttaaagcg caggcgagaa agcgcgtaag ctccgagaat gccgagaaat accgccacca
    31801 cggatgaaac caccgacacc accatgctgt tacggaagta gtcgacaaac gggaaaatca
    31861 ccgggttaaa aatgtcgaca taatgctcca gcgtccactg ttgcggcagc agcgtaggat
    31921 gcagcgagat cgcctctttt gcgctcttga acgaggtcat cagcatcaca aaaaacggaa
    31981 acagcgtgat gatgagaaac agcgccagcc cgcggtaaaa accgatacga ctgagtgtgc
    32041 gtttatttgt tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata
    32101 atgatgaaga gcaccaccga gatcgccgcc gctttcccta agtcattaaa ggcaaaggcg
    32161 gttttgtaga gatagacacc aagaatatcg accttggttg tcagcagata aacatccgcg
    32221 aacatgtaga acatccagat ggtgcgaagg gtcaccaccg tcgccaggac cggcataatc
    32281 gcgggcagcg tgacgatacg aaaccgttgc caggcattag cgccatccat ctctgccgct
    32341 tcgtacagcg atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg
    32401 aagtagcgcc agatggcgaa gatcaccacc agcacaaaac tactgcccgg attatcgaac
    32461 cacagcggtg cctgctcata aagatgcaga agatcgacgc cgaggtagtt cacaatgccg
    32521 tagccgttgt tgaacatgta tttccaggcg aacaccagcg aaatagacgg cgttacgtag
    32581 gagaggatca ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc
    32641 gccactgcca gcccgagcac ggtgctgccc gccaccacca acgcggtata ccagaccgtc
    32701 atccacagcg agtgccagaa gccgggatcg gagagaatac gcacatagtt gcttacccca
    32761 acaaacgttg actcgatgtt gggattgagc ggcagacgta aaaaactaat ttcgatattc
    32821 gacaccattg gccacgcaac cagaccgccc agcagtaata agctgggtgc gagaagcaac
    32881 agcgcaaagg gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct
    32941 gggtttagtg ctgttcgatc agttcatcca gctttttctg gctcgtttgc atcgtgccag
    33001 ggagatcggc tttacctacg gtgacgttat gcaccatgga gctcaccacc ccagaccccg
    33061 tcacatcgcc catgcgggta aattttttat cccctactgc gccaaagacc tgaatattcg
    33121 gcagttcacc gattaactga tttggcagtt cacccagcgc cttaataacg tcgttgtctt
    33181 tccaggtgga agtagtgaca accgctttgt tcaccggcag cgccgcgcct ggcgacatca
    33241 tcacccagtc ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tcagccgctt
    33301 ccgtttcttc agtcttttgt ccggcggtaa tggtcagcga ggtcaacatg ccgtagacgg
    33361 cagagttttt ctcggttggc accacgaaac cgacgttttt cggatcgcct tctttaatca
    33421 cagccggaag gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt
    33481 ctttcacttc catgatgtcg ttagaacccg gcatggtgtt ggcagcaagg tcgcggtaat
    33541 aggtcagcgc ctgcatcatc tctggtgaat caagggtgat tttgccttcg gcgttaaaga
    33601 cgttggccta gttggataac gcaaactggg agaaggattg ttccgtcagc acgctttctg
    33661 ccgtaggcag cgcaataccg tattttttat tcgccgggtc attcagtttc tgtgcaacgt
    33721 ccagcagctg ttgccagttt ttcggctcct ccagccccgc ttttgccagc acatctttgc
    33781 gataccagat gccgccaatc caggcgctga caggaacacc ggtccatgcg ctaccatctt
    33841 cggtacgcac aatacgcagt acgccatcgt aaaacgcgcc ttcaccaacg ttgctgatga
    33901 ccgtggcaac cgccttgcga tcgataagct gctctttgtc cataactttg gcgtagtcat
    33961 gactggtttc aatcacttcc ggcagcgagc cgctacgtga aagagtgatg actttggtgt
    34021 tataagcgtc ctcttcgacg ggcacttgtt taacggtgat acctgggttt tctttttcaa
    34081 aacgggcaat caatttgctg ataactgcct gacgctcctg ctccaccgaa gaatgcataa
    34141 attcgatgga tacatggggt ttattttctt ctttacagcc tgaaatcagg gcgcaggaaa
    34201 ccagtgctga taacagcacg atttttgatt taatcatgag atgttccttt ttatttaatc
    34261 cacataacct gccaggggcg cagcgttatt ttattaccca atatatcaac ttcactaatc
    34321 agatcccgac cgtgcaggcc agtaatattt acatgctgaa tatttttact gacattaaac
    34381 aatccggtca gacaattacc ctctgcgtta cttctttgaa tacgcattac ggatgaatta
    34441 accgtatcga tggtaaaatt attatccgga tgaaactcgt tatggctgcg acgaagtgta
    34501 attaaacgcg acaactcatg atataccgca tgccttaatg tagcttcatc gttcagttct
    34561 cgggttatct cttcactgtg atatttttta cggttaatcg cacggttata tccgagtttt
    34621 tcgacacctg cgtaatcatt acgcgagccc agaatacttt gaatatatat cgctggcaca
    34681 ccggggaaac ttaacaaaat cgcatgggca aggataaacc tggcgcaacg ttcttcatcg
    34741 ctactctcgc ggcggcttaa cgcatccata taggtgacat ttatttcata gggactgcgc
    34801 gtaccgtcgg gattattttt ccagtttact aatgcacctt cctgctgtaa tgcctcgacc
    34861 agcgctaata tttcgctttc tggtaacaag ccccgcagtg ggttcagccc aatgccatcg
    34921 tgagaggcga ggaagttaaa ccaggtcgtt ttaccggagg gcaagctcag actttgcgcc
    34981 cactggcaca gcgcctcgac gttctgtttt tgcaccgcat gtaacaccag cggcggcagc
    35041 gagaactggt acaccatatg cgcttcgtca tcgccttcgc caaagtaagc aatgttatct
    35101 ttatgcggaa cattcgtctc agtaataatc accgtacctg gcgcgatgtc atcaataatc
    35161 gaccgtaaca gtttgataat cagatgtgtt ttttccagat ggatgcagct tgttcccggc
    35221 tctttccaca taaagccaac ggcatccaga cggacatatt ctgcgccctt ttccaggtaa
    35281 caaagcaaaa catccaccat cgccagcaac acgtcagggc tacggtaatt caggtcaatt
    35341 tgatcgtcgc taaaggtggt ccataaatgg cgcgtggaat tatcatccat ttgaaacggc
    35401 gttaatagcg gtaatgcacg cggacgggtg acagcgctga gatcggtttg cggatcaacc
    35461 gcaataaaaa aatcttcaaa acctggctgc tgttgtaaat agtttttaaa ccattcactt
    35521 tttgccgaca tatggttgca gacaaaatca aacattaaat gactgcattc accgagctgc
    35581 tgaatatcct gccactaccc gcttcactgg cgacctgatg ataatcaatt accgaaaatc
    35641 catcatcaga tgaccatgga taaaaaggta ataaatgaac atgtgaaaaa atgctttgca
    35701 gccattgatg gtaaaactga ttaaatgtgg gtagtggttt taaatcatta ctgtgaaatt
    35761 gatcggcata ggtaattaac acgacatcac tttcatccca gtgtttttta cgtcgctgtg
    35821 taattaatcg tttcgcactc tcaagacgcg ttacaagttt ctgtaaatga gttgcggtaa
    35881 atgttccacc gtagatttcg tccaggtaat ccgtaatttt ctgtttcatg ttgcctttcc
    35941 tggtagcggt cccatgaaat gagagactac tccgcagaaa ataaactgtc aattgagcac
    36001 aagggtttta gtcacgcagg aaatcatttt ttaccaggga aaaagcgtat ttgtgagctg
    36061 acgcaaataa tctataggga tgtaaataag gtgtgacaga aaaaaacggc gcataagcgc
    36121 cgctcatggt gaattctttt aacctttgac cttcggcatt gcgatgtctt tcaggccacc
    36181 ggcgttctca acgtgggtat atcccatctc gctaaggatc tcttttgcct gccctgattg
    36241 gcgtccggca ttgcaataca ctttcacggt gtcgttttta tccggaacga cggtggcaat
    36301 gcgctctttc acttccttca ggggaatatt gatggccccc tgaacgtgct cttgctgata
    36361 ctgctctgga acacgaacat cgatccagtg ttcagcggcg aaaacgggca gtgaaaacac
    36421 cagtgccaga gcaagtaagc cttttttaaa catgtaagcg tccttctatg gatgaatatt
    36481 gctatccatt atacgcctga gatttaacaa tttttgcgaa actcccctca ccttttataa
    36541 cgctgcgcca gtttattggc agcccgactt aacagcggtt ccagcgccac agccagcaac
    36601 attttcagcg gtcggcgagc aactgatttt atcgcccagc ccgccacacc cgctgggcca
    36661 tagcgcagtg cggtaagaag taccagcttg cctgctaatt tgaaaccagg ctttaccttt
    36721 tgcccggcct gttgccagcg agtattcata actttcctca cagttgacgg aaacggctac
    36781 gtaacgtgaa tgtatcggaa gtgacataac gttccatctc gcgtaaacgc gtttcacttg
    36841 ccgccagttc acggtcgact tcatccagca attcactgct ggaaggtagc tgctcaccaa
    36901 aggccatgtt gtccggcatt ggatcaagcg caaatgacaa aatgatgtaa gcaaccaggg
    36961 taaacagcgc cagaccgaag aaaatcgaca acaccaccag gatacgcacc agttttaccg
    37021 gtacatcaaa atagttggca atcccggcgc agacgccacg gaccatgccc tgctgtggaa
    37081 tacgccataa ttttttattg agattaatgc ccgccattag cgatccctcc agttcggatg
    37141 ttctgcatca agaatagatt ccagcgcctg aatacgttcg cgcatccgtt ttgcttcatc
    37201 agccagttgc gctaatcgct gctgctcact ttgcgacaat tcactgcgac cagaacgatt
    37261 gctgtaatgc agccataacc agatcggtaa aacaaacagc acaaaaatgg ttaatggaat
    37321 agccagaaat agcgcgctca taagtactcc ttagacgatg agcggcggcg cgttcggacg
    37381 ccgcctggat gttattattg attgtcttgc ttcattttgg cttttaattg tgccagttgc
    37441 tcgctgattg catcatcggc tttcagttcg gcaaactgat cgtccagcga tttttgttta
    37501 ccgaagctgt ggctttctgc ttccgcttcc atctggtcaa tacgacgttc gaaagattcg
    37561 aaacgagcca ttgcttcatc cagtttgcca ctgtccagct gacgacgcac atcgcgcgac
    37621 gagtttgccg cctgatggcg taacatcaat gcctgctggc gagcgcgtgt ttcgctcaat
    37681 ttgttttcca gctcgccaat ctctttcttc atgcgtgcca gcgtatcgtc caccagcgtc
    37741 acttcatgtt ccagggactt aatcagatcg gtcagtttct gtttttcaat taacgccgca
    37801 cgcgccaaat cctctctctc tttcagcagc gccagttcgg ctttttcctg ccattcaact
    37861 tcacgcgccg acgcttgttc aatacggcga gtcagctgtt tcttttctgc cagcgcacgc
    37921 gccgaagtag aacgtacttc aaccaatgta tcttccatct cctggatcat cagacgcacc
    37981 agtttctgtg gatcttccgc tttctctaac agagcgttga tgttggcatt cacgatgtcg
    38041 gcaaagcgag aaaaaatacc cataatgttg tcctcttgat ttctgcattc aggattgtcc
    38101 tgctgaactg ttaatacaat ttgcgtgcca attttttatc tttttgattt ataaagatct
    38161 gattgaagaa tcaacagcaa catgccagga tgaattagct aattacacta acaagtggcg
    38221 aatttcatca tggcagaata caaagataat ttactcggtg aggcgaacag ctttctcgaa
    38281 gtgctggaac aggtttcgca tctcgcaccg ctggacaaac cggtgctcat catcggcgaa
    38341 cgcggcaccg gtaaagagct gatcgccagc cgcctgcatt atctctcctc ccgttggcaa
    38401 gggccgttta tttcccttaa ctgcgcggcg ttaaatgaaa atctgctgga ttccgaactg
    38461 tttggtcacg aagcgggggc gtttaccggt gcgcaaaaac gtcatccagg gagatttgaa
    38521 cgtgccgatg gcggtacgct atttcttgat gaactcgcta cggcaccgat gatggtgcag
    38581 gagaaattat tgcgcgtgat tgagtacggt gaactggagc gcgttggcgg cagccagccg
    38641 ttgcaggtga atgtgcggtt ggtatgcgcg acgaatgccg atctcccggc gatggtcaat
    38701 gaaggtactt ttcgcgctga cctgctcgac cggctggctt tcgatgtagt gcaactgccg
    38761 ccactacgcg aacgtgaaag cgacattatg ttaatggcgg aacactttgc catccagatg
    38821 tgtcgggaaa tcaagctgcc tctgttcccg gggtttacgg agcgcgccag agaaacattg
    38881 ctgaattatc gttggccagg aaatattcgt gaattgaaaa acgtggtgga acgttcagtg
    38941 tatcgccacg gcaccagcga ttatccgctt gatgacatca ttattgatcc ctttaaacgg
    39001 cgtccgcctg aagacgctat cgccgtttca gaaaccacct cgcttccaac actgccgctg
    39061 gatttacgtg agtttcagat gcagcaggaa aaagagttgc tgcaactcag tttgcaacaa
    39121 gggaaatata accagaagcg cgcagctgaa ttgctgggct taacctatca tcagttccgc
    39181 gcgttgttga aaaagcacca gatttagcga agttgggcaa tacaacttgc cggatgcggc
    39241 gtgaacgcct tatccggcat acataatcaa tacgtcgttt gcaggtctga taagcgcagc
    39301 atatcaggca ttattgcgtt gcccctgctt ctgcgtatta atctctcagc gcatcctgca
    39361 aaattttcat tgccgcatcg aattgcgcat ccgggatggt cagcggatac aggaaacgaa
    39421 tcacgttgcc gtatgcgcca caggtcagta gaagcagtcc ctgcgccagc gcgcgttgct
    39481 gaattttttg cgcaatggcg gctgacggct cgcccgtttg cgggtcgtta aattctgccg
    39541 caatcatcga gcccagacca cgtaccgccg cgatggccgg aacgttttct ttggcatcaa
    39601 tcagggtttt cgtcagacgc tgccccaact gcatggcgcg ctcacagagt gactctttgt
    39661 cgataatgtt gagcaccgcg tgcgcggcag ccaccgccag cggattcccg gcgtaggtgc
    39721 cgccaagccc gcccggcgcg ggcgcgtcca taatattcgc gttaccgacc acacccgaaa
    39781 gcggcatccc gcccgcgagg cttttcgcca tcgtcattaa gtccggctta tcggcgtaat
    39841 gatccatcgc aaacagtttg ccggtacgcg caaagccgct ttgcacttca tcggcaatca
    39901 tcacaatacc gtgctcgtcg cacaggcggc gaatggcggc aaccagctct tttggcgcaa
    39961 cgttgaaacc gccctcgccc tgcaccggtt cgaaaataat ggccgcgacc tgcttcgctt
    40021 cgatgtctga tttaaacaag cgttcgatgg cgtcgaggga gtcctgtgtt gaaatgccgt
    40081 gtaaatctga cggataaggt acgtgataca ccgaaccagg gaacgggccg aagccgattt
    40141 tgtacggcgc gacctttcca gttaacgcca tagtcatata cgtacgaccg tggaagccgc
    40201 cgctaaacgc aatcacgcca gggcgtccgg tatgggcgcg ggcgattttc accgcgtttt
    40261 ccaccgcttc cgcaccggtg gtgaagaacg cggttttggc ctgcccgctc accggggcaa
    40321 gggcgttgat tttctccgcc agggtgacgt agctttcata tggcacaatc tgatacgcgg
    40381 tgtgggtaaa ctgttgcagc tgctgctcca ccgccgcgac cagatcaggg tggcgatgtc
    40441 cggtattcag caccgcaatg cctgcggcga aatcgatgta ctcgttgccc tcaacatcct
    40501 tcagcgtggc gttttcagcc gactgggcga agaagttaca catcacgcca accccgcgcg
    40561 gggtggcaga aagacgacgc tgatggaatt cattgttgct catgtttcac accttttgac
    40621 tcttcactgt taatcggaaa ttaaacgccc agacgatcgc gcaggctgta atacgccgcg
    40681 cccatcgcgg taaacggtac gcgcagcgtg cgcccgccgg ggaacgggta atgcggcaga
    40741 ttggcgaagg catcgaaacg ttcggcgtcg ccgcgcagca gttcggcaat caaacgtccg
    40801 gcgagatgag tacaggtgac gccgtggccg ctgtaaccct gcatgtaata gatgttggta
    40861 tcgaggcgac cgaactgcgg catacgcgac agggtcagca ggaagttgcc cgtccagcga
    40921 tagtcgattt tcacgccctt caactgcggg aaggttttca gcagtttcgg caccacaaga
    40981 cgctcaacgt catccgggtc gcgcgcgccg tacaccacgc cgccgccgta cagcaaacgg
    41041 ttgtcggcgg taagacggta gtaatcaagc agataattac agtcttccac gcagtagttt
    41101 ttcgggatca gcgaacgggc taaatcatcg gaaagccgct cggtggtgat cacctgcgta
    41161 ccgcacggca tgctgcgttt cgccagttcc ggctccactt tatcgcccag gtacgcattc
    41221 ccggcgacaa tcacatactt cgccgtcacc tgacctgtgg ctgtacgcac tacggcgggc
    41281 gtggtgtgct ggatctgcgt cactgcggaa agctcataca cgcgcccgcc gttcaggcgg
    41341 atagcgtccg cttcgccaat ggcgaggttc agcgggtgaa tatgcccgcc gctgtgatcc
    41401 agcagcgcac cagtgtagcg atcgctggcg acctcgcggc gaatggcgtt ggcgtccagc
    41461 atctccagct gtttattgcc gtagcgttcc cagttctctt tctgctcttc aagtgtggcg
    41521 agctgtttat cattcatcgc cacaaacaga ccgccggggc ggtagtcgca gtcaatctga
    41581 taacgtttga tacgttcgcg gatgatctca ccaccttcga acatcatgct gccgagcata
    41641 cgggcggtgt ccatgccgta gcttttttcg ataacgtcga tgtcgcggct gtaggagttc
    41701 acaagctgcc cgccgttgcg cccgctggcg ccaaagccga tgcgtgaggc ttccagaacc
    41761 accacgtcaa agcccgcttc cgccagatgc agtgcggaag acagcccggt atagccgccg
    41821 ccaaccacgc aaacgtcgca ggaaattgac tcattcagcg tgtcgaatgg cgcgtattta
    41881 ttcgcactgg cggcgtagta actgctggta tgttctgtca tttcaggcct ccaggcttat
    41941 ccagatggtt ttcagttcgg tgaatttttc aagggcatgc agggatttgt cgcgaccgtt
    42001 gccgctctgc ttatagccgc caaacggcac ggtcatatcg ccgtcgttgt agttattgac
    42061 gaagacggaa ccggctttca ggcgtcggct catgcggtgc gcgcgggaga ggtcgcgcgt
    42121 ccataccgcc gcgccaaggc cgtactggct gtcgttggca agctgtagcg cctgttcttc
    42181 tgatgtgaaa cgcgtgacca ccagcaccgg accgaaaatc tcttcgcggc ttaaggacgc
    42241 attcgggtct acatccacaa tgatggtcgg gccgatggcg acagccagcc cggcgttacg
    42301 gccatccaac aacagttgcc ctttgctttc gccttcctga ataaagctat ggaccgagtc
    42361 ggcgtgggcg cagtcgatta aggtgcccat ggtggttgcg ggatcaagtg gatggcccgg
    42421 ctgccagttt tgcgcctgct gttttaacag ggctaagaat tcatcggcta tgctctcttc
    42481 cagcaacagg cgcgttccgg cgatgcacac ctgcccctgg ttgtagaaga tgcctgctgc
    42541 ggtggcgctt gccgcctgtt gcaaatccgg gcagtcagcg aaaacgatgt tggcgctttt
    42601 gccgcccgct tccagccaga cgcgtttcat gttgctgtcg cctgcatctt tcagcagctg
    42661 tttcccggta cgggtcgaac cggtaaaggc aatggcgtcg atatcgttat gacgcgacag
    42721 cgcctgcccc gcttcatgac caaaacccgt caccacgttc aacacaccat ccggcaagcc
    42781 tgcttctttc gccagccccg cgagacgaat cgcactgagc ggtgattttt cagacggttt
    42841 caggacaacg ctgttccctg ctgccagcgc cgggccaagc ttccagcaag tcagcagcag
    42901 cgggaagttc cacggcacga tggcggcaat cacgccgacc ggttcacgca cgatcatcgc
    42961 cagctcatgg ctactggtgg tcgccacttc gccatacact ttgtcgatcg cttcggcgta
    43021 ccagcgaatg gcgcgcgccg cgccgggtat atcatcacgc agactgtgac gaatcggttt
    43081 gccggtgtcg agagtttcca gcagtgccag ctcttcggcg ttggcttcta ttaaatcggc
    43141 gagtttattc agcaccgctt tacgttttgc cggagaagag agtgaccagt cgccgcgttc
    43201 aaatacgccg cgtgctgcgc tcaccgcacg gtcgatatcg acgctcttgc cgcgcgcaat
    43261 attcgccagc ggtgcctggg taaccggatc aacggtttca aaggtttcat tttccgccgc
    43321 agcagtatat tcaccgttaa taaataagcg gttttcaatg gcgagactta acgctttatc
    43381 ctgccagtaa gccagatgat gaaaattcat tatgactcct gtttcacgtc tatcagatat
    43441 atgcagtttg tgaacgtcag ggcgcagaga tccccgggcc gtatgcccgt ttatttctgg
    43501 cgacattctt tatggattgc cgggcgcatt gcgcttaccc ggcttacaga acaaaaagat
    43561 taaaacgtgg tgggcgtatg ggcgctgata attcggcaaa tacctgccga cgtattactg
    43621 aaactgtgcg ggatgccggt attaatggca taactttgcc ccgcgacgag gtggtaatcc
    43681 tgaccattaa tcgtcagaat aatttcacct tccagtacag tgcctatttc ctcaccctga
    43741 tgcttaattc tttccccagt ggttgtgccc ggctggtacg tttcaaagat catcgccagc
    43801 gtgcgattcg ggttaccgtt atgaaccagc ttcattgaca caccctgact gcccatctca
    43861 attaaatcgt cctgattaat gacgacctgc ggctcatcag gtttttccgg ctcggaaaag
    43921 aattccgaga gtgacagacc atacaccttc agcagctttt gcagcgtact gatggcaggg
    43981 ctgactttat cttgttctat cgtactgata gcactgtgag tcagcccgga gagttcggcg
    44041 gcacgacgtt gtgaaagccc ctgctgctgg cggatttccg acaagcgttt tcctggcgcc
    44101 agtccctcat cactcatatt tgcatttcct taaactgtag tggtcagagt cgctgttttt
    44161 cagcgatatg gtgctgacaa gcggtgataa agccctcgaa caatatacgc gaaagcgcgt
    44221 actcgctact gttccattcc gggtgccact gtacgcccag cgcaaaagga tgattgatga
    44281 cactaaccgc ctcaactaag ccatccggcg agcgagcttc aacacgcaac cgtgggctaa
    44341 cgaccttcgc cccttgtcca tgcagagagt ttacccaaaa gttgctacat tcaggtaaca
    44401 atgcagacag caatcccccc tcttcaacct gaacttcatg cgacggtgca tattgctgtt
    44461 ccaccggcag ttcgggatct tcccgatgtt ccagcaattc aggctgttcg cacagcttgc
    44521 gatgcaacga accaccggtt gccaccacca gttcctgtaa accccggcag atggcgaaaa
    44581 tggggatgcg cctttcgagt gcggcattaa ttaacgccat gctcagaaga tcacgctcgg
    44641 gatcggcgtc aggctcatcg ccgttttcac catataggtg cggctgcaca ttgctgggac
    44701 taccaggaag gtaaatgcca tcgagtttcg gcaaaagttg ttcaagtaac gacggttccg
    44761 ccagcgcatg tggcagcgca attggcaagc cgcctgcgtg gatgatggca ttcaggtact
    44821 tttcttgcag agtctgggtc gcatgcccct taagcctgtt cctgcacatt acgacaccga
    44881 taaccggatt gttcattata ttttccattc tcaatctcac aaagtggact aaattatcgc
    44941 cattactgcg gtttaaatga cctttatgtt caatattttt tcaatctagc agtgggtttt
    45001 catttttgca aactcaattt aacatttgac aaacatttag tttgcataca gattcgaatg
    45061 gtggtcatta tattttacgc tttgataacg agcggaaaac aaaccaaagg cgaagaatca
    45121 tggaaaccaa tatcgttgaa gtagagaact ttgttcagca gtcagaagag aggcggggta
    45181 gcgcctttac gcaggaagtg aaacgctacc tggagcgcta cccgaatacg caatatgttg
    45241 atgttttact taccgatttg aatggttgct tccgtggaaa acgtattcct gtttcaagcc
    45301 tgaagaagct cgagaaaggg tgttatttcc cggcctcggt atttgcaatg gatattctgg
    45361 gcaacgtagt agaagaagca ggtctgggtc aggaaatggg cgagccggat cgtacctgtg
    45421 ttcctgttct cggttcctta accccttctg ccgccgatcc agagtttatc ggtcagatgc
    45481 tcctgaccat ggtcgatgaa gatggcgctc cctttgacgt tgagccgcgg aacgttctta
    45541 accgcctgtg gcagcagctg cgccagcgcg gattgttccc ggtcgtagcg gtagagctgg
    45601 agttctattt actggatcgc cagcgcgacg ctgaagggta tctgcaaccg ccctgcgcgc
    45661 ccggcactga tgaccgcaat acacaaagcc aggtttactc cgttgataac ctcaaccact
    45721 tcgccgacgt gctcaatgat attgatgaac tggcgcagtt acagctgatt ccggcagatg
    45781 gcgcggtcgc tgaggcctcg ccgggtcagt ttgaaatcaa cctttaccat actgataacg
    45841 tgctggaagc ctgcgatgat gcgctggcac taaaacgcct ggtgcgtctg atggcagaaa
    45901 agcataagat gcacgccact tttatggcga agccgtatga agagcacgcg ggcagcggaa
    45961 tgcatatcca tatcagtatg caaaataatc gtggcgagaa cgtgctttct gacacagaag
    46021 gcgaagattc gccgctgctg aaaaagatgc tcgccgggat gattgacctg atgccgtcat
    46081 caatggcgtt gctggcacca aacgtgaact cgtatcgccg cttccagccg gggatgtatg
    46141 taccgacgca ggcgtcgtgg ggccataata accgtaccgt cgccctgcgt attccatgcg
    46201 gcgatcgtca taatcaccgc gtggaatatc gcgtggcggg tgccgatgcc aacccatatc
    46261 tggtgatggc agcgattttt gccggtattt tgcatggcct tgataacgag ctgccgttgc
    46321 aggaagaagt cgaaggtaac gggctggaac aggaaggctt acccttcccg attcgccaga
    46381 gcgatgccct gggtgagttt atcgagaatg atcacctgcg ccgctattta ggcgaacggt
    46441 tttgccatgt gtatcacgcc tgtaaaaatg atgagctgtt gcagtttgag cgcctcatca
    46501 cagaaacaga aattgagtgg atgttgaaaa acgcatagta ccccttgcgg ggttgtttca
    46561 tcggcctgag cggagccagg cctgacattt gctggtaatc cagacatttc ccggatgtcg
    46621 cgttatggag cgcaggcagc aacgggcttc cgttaacgat taagactgtg acgaggaaat
    46681 gagatgatgc cgatgattaa atccccgcat ggcgcgggag gctgcgtatg cgcccctcct
    46741 gcgactgact ggactccgcc ccctttgctt cccttgctaa acaggtttga tttcaggtcg
    46801 acacgaccgc aaaccttatt acgcagggga ggcagcaatt atggctatta attcaccact
    46861 gaatattgct gcgcaacccg gcaaaacccg tctgcgaaaa tcactgaaat tgtggcaggt
    46921 tgtgatgatg ggtctggcct atctcacgcc gatgactgtg ttcgatacct ttggcattgt
    46981 atctggcatt agcgacggtc acgtccctgc gtcctatttg ctggcgctgg cgggcgtgct
    47041 gtttaccgct atcagctacg gcaaactggt tcgccagttt ccggaggccg gttcggccta
    47101 tacctacgcg caaaagtcga ttaacccgca cgtcggattt atggtcggct ggtcatcact
    47161 gctggattat ctctttttgc cgatgatcaa cgttttgttg gcgaaaatct atctctccgc
    47221 cctcttcccg gaagtgccgc cgtgggtgtg ggtggtaacc ttcgtcgcca ttttaaccgc
    47281 cgcgaatctg aagagcgtca acctggtcgc taacttcaat accctgtttg tactggtgca
    47341 aatctccatc atggtggtgt ttatcttcct ggtggttcag ggactgcata aaggagaagg
    47401 cgttggcacc gtctggtcac ttcagccgtt tatcagcgag aacgcgcacc tgatcccgat
    47461 tattaccggg gcgacgattg tctgtttctc gttcctcggt ttcgatgcgg tgaccacact
    47521 ttcggaagag acaccagacg ccgcacgcgt gatcccgaaa gccatcttcc tgacggcggt
    47581 ctatggtggc gttatcttta tcgcggcgtc gttctttatg cagctgttct tccccgatat
    47641 cagccgcttt aaagacccgg acgccgcact gcctgaaatt gcactctacg tcggcggtaa
    47701 gctgttccag tcgattttcc tctgcaccac gtttgtgaac acgttagcgt ctggcctggc
    47761 ctcacatgcc agcgtgtcac gtctgctgta tgtgatgggg cgcgacaatg tgtttccgga
    47821 gcgcgtgttt ggctatgtgc acccaaaatg gcggactccg gcactgaacg tcattatggt
    47881 cggaattgtc gcgatgtcgg cattgttctt tgatttagtc accgcgacag cattgattaa
    47941 cttcggtgct ctggtggcgt ttaccttcgt gaatctgtcg gtgtttaatc atttctggcg
    48001 gcgtaaagga atgaataaaa gctggaagga tcacttccac tatttgctga tgccgctggt
    48061 tggcgcgctg acggtgggtg tgctgtgggt taacctcgag tcaacgtcac tgacactcgg
    48121 tctggtatgg gcttcgttag gcggcgcata tttgtggtat ttgatccgcc gctatcgcaa
    48181 agtgccgctg tacgaaggtg acagaacgcc agtgagcgaa acgtaatagt cggatgcgcg
    48241 ccgcattatc ctgcctgagg gtaatgcgcc gcgccattaa ctataaaaag atgtatgacc
    48301 cggatcacga gtgctatcat ccgcccttat gcttagcgca ttaaccccag gggcaaataa
    48361 tgaaccacga tatcccgcta aaatattttg atattgccga tgagtacgcg accgaatgcg
    48421 cagagcctgt cgcagacgct gaacgcacac cgctggccca ctacttccag ttactgctca
    48481 cccgtttgat gaacaatgaa gagatcagcg aagaagccca gcatgaaatg gctgctgaag
    48541 cggggattaa tcccgtacgc attgatgaga tcgcagagtt tttgaatcaa tggggtaatg
    48601 agtaattatt taattgcaga cgatcggtca gtatcgttgt ccggataaag cgttcacgcc
    48661 gcatccggct aacaatgaca cgcactgcag cgtgctttaa tgggtttctc acgatcgtac
    48721 cgctctgctc ctcgctaagt tgttctgtga gttatagtta tagagaaatc acgggtacga
    48781 atgttgaaca gatggcgacg ccatctgttc aacgaccaac cacacggccc tcattacgac
    48841 ccctgaacac aaacggtaat ggaaaatgtc attaaatatt agtaagatag cctctttaca
    48901 taaaatccct taatattatg cgccaggtat tatcgtctct tttggtgatt gctggacttg
    48961 tgagtggtca ggcaatcgcc gcgcctgaat cccccccgca tgctgatatc cgcgacagcg
    49021 gttttgtcta ttgcgtcagc gggcaagtca acacctttaa cccatccaaa gcgagcagtg
    49081 ggttaattgt cgataccctt gccgcccagt tttatgatcg actgctggat gtcgatccct
    49141 atacctatcg cctgatgcca gaacttgccg aaagctggga agtactcgac aacggcgcga
    49201 cctatcgctt ccacctgcgt cgcgatgtcc cgtttcaaaa aaccgcctgg tttactccca
    49261 ctcgcaaaat gaatgccgac gatgtggtgt ttaccttcca gcgaattttt gaccgcaaca
    49321 acccgtggca taacgtcaac ggcagcaact tcccctactt cgacagcctg caatttgccg
    49381 ataacgtgaa aagcgtccgt aaactggata atcataccgt tgagttccgt ctggctcagc
    49441 cggatgcttc ttttttgtgg cacctcgcaa cccattatgc ttcggtcatg tcggcagaat
    49501 atgcccggaa gttagagaaa gaagatcgcc aggagcaact cgaccgtcaa ccggtcggca
    49561 ctggaccgta tcagttgtcg gaataccgcg ccgggcaatt tattcgccta caacgtcatg
    49621 atgacttctg gcgcggtaaa ccgttaatgc cgcaggtggt ggtggattta ggctccggcg
    49681 gcaccggacg tctgtcgaaa ctcctgaccg gggaatgcga cgttctggcc tggcctgctg
    49741 ccagccagct atccattttg cgtgacgacc cgcgcttgcg tttaacgctg cgtcctggga
    49801 tgaacgtcgc ctatctggca tttaacaccg ccaaaccgcc gctaaataat cccgctgtcc
    49861 gccatgcgct ggcactggcg attaataacc agcgcctgat gcaatccatc tattatggta
    49921 cggctgaaac ggcggcctct attttaccgc gcgcctcgtg ggcctatgac aacgaggcta
    49981 aaattactga atacaatccg gcgaaatcgc gcgaacagtt gaagttgttg gggctggaaa
    50041 atttaacgct gaaactgtgg gtgcccacac gttcgcaggc gtggaacccc agtccactga
    50101 aaactgccga actgattcag gcggatatgg cgcaggttgg cgtaaaagtg gtgattgtgc
    50161 cggtagaagg tcgctttcag gaggcgcggt tgatggatat gagccatgat ctgacgttat
    50221 ccggttgggc gacggacagt aacgacccgg acagtttctt ccgtccgtta ctgagctgcg
    50281 cggcaattca ttcacagacc aacctcgccc actggtgcga tccgaaattc gacagcgtgt
    50341 tgcgtaagac gctctcctcg cagcagctgg cggcgcgtat tgaagcctat gacgaagcgc
    50401 agagtattct ggcgcaggaa ttgcccattt tgccgctggc gtcgtcattg cgtttgcagg
    50461 cctatcggta cgatatcaaa ggtctggtac ttagcccgtt tggtaacgcc tcctttgctg
    50521 gggtgtatcg cgagaaacag gatgaggtga aaaaaccatg attatcttca ccttacgccg
    50581 cattttgtta ttgattgtca ccttgttcct gctgaccttc gttggcttca gcttgagcta
    50641 tttcactcca cacgcgccgt tacaaggcgc gtcactgtgg aatgcctggg tgttctggtt
    50701 taacggcctg atccactggg attttggtgt ttccagcatt aacggtcaac caattgccga
    50761 acagttaaaa gaggtcttcc cggcgacgat ggaactgtgc atcctcgcct tcggttttgc
    50821 gctgattgtt ggcatccccg tggggatgat tgccgggatt acgcgccata agtggcagga
    50881 taacttgatc aacgccatcg ccttactggg cttttcaatc ccggtgttct ggctggcgct
    50941 tctgttgaca ctgttttgtt cactcacgct aggttggttg cccgtttcag ggcgtttcga
    51001 tctgctctac gaagtgaaac cgattaccgg ttttgcgttg attgatgcct ggctttcgga
    51061 ctcaccatgg cgggatgaaa tgatcatgag cgcaatccgc catatgatat tgcccgtgat
    51121 caccctgtcg gttgcgccaa caactgaagt gatccgcctg atgcgtatca gcactatcga
    51181 agtgtatgac cagaactatg taaaagcggc ggcgacccgc ggtttgtcgc gctttactat
    51241 tttgcgtcgc cacgttctgc ataacgcgtt accgccggtt attcctcgcc tgggcttaca
    51301 gttttccacc atgctgacgc tggcgatgat caccgaaatg gtttttagct ggccgggttt
    51361 agggcgctgg ttaattaacg ccatccgcca gcaggattac gcagccattt ccgccggagt
    51421 gatggtgtgt ggctcactgg tcattattgt taacgtgatt tctgatattt tgggtgccat
    51481 ggctaaccct ctgaaacata aggaatggta tgccttacga tagcgtatac agcgaaaagc
    51541 gcccgccggg cacgctgcgt accgcctggc gcaaatttta tagtgacgcc tctgcgatgg
    51601 tcggcctgta cggctgcgcg gggctggctg tactgtgtat gtttggcggc tggtttgcgc
    51661 cttacggcat cgaccagcaa tttctcggtt accaattatt gccgccatca tggtcgcgct
    51721 atggcgaagt ttctttcttc ctggggactg acgacctcgg acgcgatgtt ttaagccgtt
    51781 tactgagcgg agctgcgcca accgtgggtg gcgcatttgt ggtgacactt gccgcgacga
    51841 tctgtggcct ggtactgggc accttcgccg gggcgacgca cggcctccgc tcagcggtgc
    51901 ttaaccatat tctggatacc ttactggcga tcccttccct gctgttggca attatcgttg
    51961 tagcgtttgc cggaccgagt ttgtcccacg ctatgtttgc tgtctggctg gcgctgctgc
    52021 cgcgtatggt acgttcgatt tacagcatgg tgcatgacga actggaaaaa gagtacgtta
    52081 tcgccgcccg tctggatggc gcatcaacgc tgaatattct ctggtttgct gtgatgccta
    52141 acatcaccgc cgggctggtc accgagatca cccgcgcact gtcgatggca attctcgata
    52201 tcgccgcgct gggctttctc gatcttggcg cacaactccc ctcgcctgaa tggggagcga
    52261 tgctcggtga tgcgctggaa ctgatttatg tcgccccgtg gactgtcatg ctgccaggtg
    52321 cggcaattat gattagcgtg ctgttggtta acctccttgg cgacggtgtc cgtcgtgcaa
    52381 ttattgcggg ggtggaataa tgccattact cgatattcgt aacctgacta ttgaatttaa
    52441 aaccggtgat gagtgggtta aagccgttga ccgggtaagt atgaccttaa ccgaaggtga
    52501 aatccgcggt cttgttggtg aatccggttc cggcaaaagt ttgattgcga aagcgatttg
    52561 tggggtgaat aaagataact ggcgtgttac tgcagaccgt atgcgttttg atgatatcga
    52621 tttgctgcgt ctctccgcac gcgaacggcg caaactggtt ggccacaacg tgtcgatgat
    52681 tttccaggaa ccgcagtcgt gtctagatcc ttcagaacgt gtgggtcgcc agttgatgca
    52741 aaacatccca gcctggacct ataaaggccg ttggtggcag cgttttggct ggcgcaaacg
    52801 ccgtgccatt gaactgctgc accgcgtggg aattaaagat cataaagatg cgatgcgcag
    52861 ttttccctat gagttgaccg aaggtgaatg tcagaaagtg atgatagcca ttgcactggc
    52921 gaatcaaccg cgtctgctga ttgctgacga accgacaaac tcaatggagc caacaaccca
    52981 ggcgcaaatc tttcgcctgc tgacgcgtct aaaccaaaac agcaatacca ctattttgct
    53041 tatcagccat gacttacaaa tgcttagcca atgggcggat aaaattaacg tgctttactg
    53101 cggtcaaacg gtggaaaccg cgccgagtaa ggagttagtg acgatgccac atcatcctta
    53161 tacccaggcg ctgatccgcg cgataccaga cttcggaagc gcgatgccgc ataaaagtcg
    53221 cctcaatacg ctacccggcg ctatcccact gcttgaacag ttaccgattg gttgtcgtct
    53281 ggggccacgt tgcccgtatg cacaacgaga atgcattgtg acgccacgtt tgacgggggc
    53341 gaaaaatcat ctctatgcct gtcatttccc gctgaacatg gagaaagagt gagatgatcg
    53401 aaacgctgct tgaagtgcgt aatttaagta aaacgttccg ctaccggacc ggttggtttc
    53461 gtcgtcagac cgtagaagcg gtaaaaccct tgagctttac gctacgtgaa ggccagacac
    53521 tggcgattat tggcgagaat ggttcgggta aatccacgct ggcaaaaatg ctggcgggaa
    53581 tgatagaacc taccagcggc gagttattga ttgacgatca tccactgcat tttggcgatt
    53641 attccttccg cagtcagcgt attcgcatga tttttcagga tccttcgacc tcgttgaatc
    53701 cccgtcaacg tatctcgcaa attctggatt ttccactgcg cctgaacacc aacctggaac
    53761 cggaacagcg tcgcaaacag attattgaaa cgatgcgtat ggttgggctg ctaccggatc
    53821 acgtcagtta ctatccgcat atgttggccc ccgggcaaaa acagcgtctg ggtctggcgc
    53881 gcgcgctgat attgcgccca aaagtcatta ttgccgatga agccctcgct tcactggata
    53941 tgtcgatgcg ttcgcagttg attaatctga tgctggaatt acaggaaaaa cagggcattt
    54001 cgtatattta tgttacccag catatcggaa tgatgaagca cattagcgac caggtgctgg
    54061 tgatgcatca gggcgaggtt gtcgagcgag gcagcaccgc ggatgtgctg gcatcgccgc
    54121 tgcatgaact caccaaacgg ctgattgccg ggcactttgg cgaagcattg acggcggatg
    54181 cgtggcgtaa ggaccgttag tgttctgcac ctcatcctga caggggaacc cccctcagga
    54241 tgaggtattc attacttttg attgtcgatt ctggtgagag caaccaggaa tgccagtgta
    54301 taacacaccg taattgccag actcattttt aacagtgttt ctccgccaaa tcccgacaat
    54361 ggcgcggcta taccgccgaa gacaaacatg ctcatcccca tcaacgcaga cgccgttcca
    54421 gattcctgcg tccctactgc actcatagcc tctgccccgc caacagtgcc gatgccgcta
    54481 caaaatgcaa tagtgaaaaa taatgccacc agtgccggaa tgggcaattg tgtccatgcg
    54541 cataagaccg tgagcaatgc acacaaaatt gccgctatca ggccacctcg caacaatgtc
    54601 atcgcattaa tccgtcgcgc cagacgcgaa aagatccatg aagcaatgat aagtccgatg
    54661 ccgttaaggc caaacaccag gctaaattgc attggactaa agccaaattc cttctgcaac
    54721 acgaacgacg aagagccgat atacgcaaac aagcctgcca gcataaaact ttgaatcagg
    54781 caaaagcgca taaaacggcg gttctgcacc acgcttcgtc cggttaatag caatgatgag
    54841 ccccttttat tttctggcaa ggtctcatta ataaataaca cgcagcccag caacaatacg
    54901 gtgctaattt cagccattac ccagaataaa gtgcgccagt caaaagtgct aacaatgtac
    54961 ccgcccagca ctggcgacaa caccggtgcc aggccattca ccgtcattaa cagcgcaaaa
    55021 aactgggtca acgttactcc ctgatatttg tcacgagcaa tagaacgaga gagcaccgaa
    55081 ccacccgccc ccgcgatccc ttgaataaaa cgccagacca ccagccagta aatattgttc
    55141 gttgtcgcgc acaaaatgga agaaacaata aacaatagca acgacaagat taacggacgt
    55201 ttacgcccta ttttgtcgct cagagggcca aataacagtt gtccgacgcc aagaccgatc
    55261 agtgatgcag tcagcgttaa ttgcgttatg gtcgtggttg ccgccagttg ctcagacatc
    55321 tccggcaaag ccggcaaata aagatcggta cacatcgggc cgataccggc taaaagacca
    55381 agaatcaatg cccatgaaag agagactcta gccatattat tccttacttg agtgatgaca
    55441 tgccgccgcc cagcgactgc cagagcgtga ttcggtttgt aaaatcagtc agttgcagtg
    55501 ccagcagctc ctgctgtgcg ctccacaagc tgcgctgcgc cgtcaacacg gtcaggtaat
    55561 caccaacacc cgtctgatag cgacgcaagc caacatcgac cgtttgttgc tcagctttaa
    55621 catactggcg ctgtgcatcc agttgctctt caagcgtggt ccgccgcgcc agtgcgttag
    55681 cgacatcttt aaatgctctc tgaacggttt tttcataggt ggcaatgagt ccacgctttt
    55741 gtgcttctgc gtaacgaagc tgcgccagat tacttccgcc agtgaacagc ggtaatgtga
    55801 ccgatggcgt aaatgaccag atctgcattc cgtggctgaa caacgacgac aatgcgtcac
    55861 tgccaacacc agcgctggca gtcagcgaaa tcgtcgggaa aaagttagcc cgcgccgcgc
    55921 caatatcggc attggcgctc ttcaaattat gttccgcttc ctggatatcc gggcgacgca
    55981 gcagaacgtc agatgacact cccgcaggaa ccagacttat catctgttcc ggcaggcttt
    56041 ccagtgttcc cggtagcaaa ttttcctcaa gcgttgtacc tgccagtaag ttcagcgcat
    56101 ttttatcctg catcacttgt gtctgatagc tggcaacgct ggcgcgcgct tgctgataaa
    56161 cactcatcgc ctcactgaca tctgtcgctg ccgccgtgcc aacctgttgc tggcgctgga
    56221 taattttcaa tgagttttct gcactggtca tcgtttcttt tgccagggcc aggttgctgt
    56281 tatcggccgc cagtgtcagc caggcggtgc tgatttcagc aatcagcgtt aaacgggtgt
    56341 tctgggctgt aaattcactg gcaagccagg tttctcgcgc cgcccgagat aaactctgat
    56401 tacgaccaaa cagatctaac gtaaaactgg acaccgtgcc gtcagcctcg gcagtggttt
    56461 ccgtaccatt cgccagcgaa cgactgcggg ttgaacttaa ggccgcattc accgtcggga
    56521 ataacgacgc attagtttgc ccatagagcg cccgtgctga gtcaatatca gcaatcgctt
    56581 tttgcacatc gcggttactg ttcagtgcaa tagtcaccac ctgctgcaat cggggatcgt
    56641 ggatcacctg ctgccagtca tggctgattg cctttccctg gccctgtgct cccggcaagg
    56701 tcgccgggat tggggattca ggcgtgctgt agtggggatc gagcgagaca catcccgcac
    56761 tcaacagcac cagaaaaata agagaacgac gcaacataac ttactcctga cggcgcgatt
    56821 taccggcaaa caaacgcttc accagtacaa aaaacagagg aacaaagaaa atagccagca
    56881 acgtagcggt cagcgtaccg ccaataatgc ccgtaccaat ggcgatgcgg ctgttcgccc
    56941 ctgagccggt tgctatcgcc agcggcatta ccccccgcaa taaacgccag cgaggtcatg
    57001 atgattgggc gtaaacgagt ctgcgccgcg cgtaatgccg cacgactcaa ggaataaccc
    57061 tctgcaaccg ccgcttcagc aaattccaca atcagaatgg cgtttttcga cgacaagccg
    57121 atagtggtta atagcgccac ctggaagtaa acatcgttgt ttaaatcacg catccaggcc
    57181 gccagcgccg cgccaagaag ccccagaggg atcaccagaa tgaccgagaa cgggactgac
    57241 cagctttcgt acagtgctgc aaggcagagg aaaaccacca gaatagaaac tgcatacaga
    57301 ctcaatgcct gaccgctggc cagtttctcc tgcaatgaca aaccactcca ggcccaggtt
    57361 gttcccgcag gaagactgtt tgccagttcc tccatttttg tcattgcatc gccggaacta
    57421 aagccagtcg cattttcccc ctgaatttca taggctgcag aaccgttgta gcgcaccagt
    57481 ctttccggtc catacagcca gcgggtggtc gcaaaagcag agaatggtgt catggcgtta
    57541 tcactaccgc gcacaaacca tttacctaag tctgacggcg cggaacgaaa ttcactgtcg
    57601 ccctgaatgt agactttttt cacacgacca cggtcaataa agtcattcac ataagtaccg
    57661 ccccacgcac tggacagggt gtcggtgaca tcattcaaac tcaaccctaa tgacaccgct
    57721 ttattactat caatatctac ctgcaattgc ggcatttgtg gcaaatcatt ggcacgaaca
    57781 gaatgtaact ccggactttg attcgctttt tctatcaact gattacgcat ttgcagtagt
    57841 gtttcgcgat cggttccacc gttagctaac aattcaaacg taaaaccatt gctttgcccc
    57901 agcccatcaa cggctggcgg cgtcatcgca aataccgtgg catcacgaat tgtgcccagc
    57961 tctttggttg cccgtagggc gatagcctgg gcggtgtttt ctgccccttt acgttgagac
    58021 cagtttttca acgaaacaaa cgccatcccg gtgttctgtc cgctgccgct aaaactgaaa
    58081 ccatcaacgg taaaaatgac atcggtattt gctttctcgt taatcaggaa ccagtcaaca
    58141 atctggcgat tcacttctgc tgtacgggca gcggtcgccc cggccggcag cgtgtactgc
    58201 accatgattt cgccctggtc ttcagtgggt aagaaactgc ccggcagttt ccacatcatc
    58261 agggccattc caccacccag tacgagataa agccccatcg ttcgagcagc acggcgcagg
    58321 acataaatta cgcctcgctg atatttatct tcagtacggc ggtaaaagcg gtcaaatgcg
    58381 ccgaaaaagc cttttttatg tggcggaaca tgctggagga cggaaccgca cagggcggga
    58441 gtcaaggtca atgccaccac cacggaaagc agcattgcgg agataatggt tatcgaaaac
    58501 tgacgataaa ttacccccgt agatccccca aagaaggcca taggcaggaa tacggctgac
    58561 aacaccagcg caatggcaac cagtgcgcca gaaatctcgc ccattgattt ttccgtggct
    58621 tcacgcgcgg gcaacccttt gtcacgcata atgcgttcga cgttctccac taccacaatg
    58681 gcatcgtcca ccagcaagcc gatcgccagc accatagcaa acaacgtcag ggtattaatc
    58741 gaatagccaa acaacgcaag aacgccgaat gtgcccagca aaacgacggg gacagctaac
    58801 gccggaatca gtgtggcacg caggttttgc aggaataaat acatcacgca aaccaccaat
    58861 acgatggctt caaacagcgt ctgaattacg tcctctacag agattttaat aaattcagtg
    58921 ctgtctttcg ggtaagcgat gtcgtatccc tgcggcatgt tccgctggaa ttcggcaatc
    58981 ttatccttga ccagcgtcgc cgtattcagc gcattcgcac cgggcgaaag cattacagca
    59041 accccggcag acgggtgacc gttaagtttg ccgatagcgg tataatcttc actgcccatc
    59101 tcaacgcgag ccacatcttt tatacgaaca actgcgctgt ctgactggct tttcacgata
    59161 atatttttga actgatccac cgtctgcaag cgagactgcg cacgaaccgt tgcggtcagt
    59221 tgctgagtat tcggtgaagg caatgcccct attttcccgg cagtaacctg cacgttttgc
    59281 gcttcaatag cactttgcac gtctgaaggc attagcgagt aagacgccag tttggccggg
    59341 tcaagccaga tgcgcatagc gtattccgcg ccaaagactt gcagactccc cacaccttca
    59401 acacgcgcca gcgggtcctg aacgttacta accaaccagt cggcgatatc cgaactggag
    59461 gctttgtcag tggtgtcata cacggcagca atcagcaaaa agttgctttg tgatttctcc
    59521 accgtaacac ccgtttgctg cacttcgctg ggtaggcgcg actccgcctg ctgaatttta
    59581 ttctgcacct gaacctgtgc agtatctgga tcggtacctt gttcaaaggt cacattaata
    59641 ctgaccgaac catcagagct gctggttgag ctgaaataga gtaaattatc aagcccagtg
    59701 agttgttgct cgatgacctg agtcacgctg ttctccagcg tttcggcaga agcaccagta
    59761 taagtggctg aaattttaat ggtcggtggc gcaacgtcag gatattgcgc gacgggcaat
    59821 gtgcgaatag ccagaatccc cgccaacata atcaaaatgg cgataaccca ggcaaagacc
    59881 gggcggcgaa caaaaaagcg cgaaaacatc aggcgttgcc tccattagcc tgaacttcaa
    59941 cagccttgac cgtctgccct gaagtgactt tggcagaacc ttcaacaatc agtcggtcgc
    60001 cgctgtgcag gccgtttagc accagccatt tatcaccata tgtttctccc gtttcgagcg
    60061 ttcgctgctc tactttattg tctttattca ccaccagcgc agttgcattg cctttagcat
    60121 cgcgcgtgac gccctgttgc ggcgcgagaa tagcgtcttc catcacgcct tcatcgacac
    60181 gagcgcgaac aaacattccc ggtaataatt gctgttgtgg attggggaaa atcgcccgta
    60241 atgtcaccga accggtagac tcatctacag cgacttcggt gagttccaga cgcccttttt
    60301 cgctgtaggt tgtgccatct tccagaataa gtgagacgct catggtgtca ctgttggtcg
    60361 ccagtgactg tttacgcaga cgtaataaat cgacactgga gcgagtgagg tcgacataca
    60421 ttgtatccag accacgaata gtcgttaacg ctgtatcttg cgacgcggtc accagtgcgc
    60481 caggggttac cgacgaaatg ccaatgcgcc ctgaaatcgg tgcggttacc gtggtccagt
    60541 caagattaat gcgcgcagtt tccagtgcgg cttttttcgc cgctacactg gctttatctt
    60601 gtgcacaggt agactgagca tcatcagcat cctgttgtga aacaccgttc tctttcacca
    60661 gtcgggcata acgctgcgct ttctggcaat cggcttttac cagtgcctgt gcttgttgta
    60721 atgctgcccg agcttcattc catgcagcct gataactggc cgcatcaatc tggtagagcg
    60781 gctgtccagc cttgaccaga tcaccttcct taaataagcg tttctggata attcccccaa
    60841 cctgcggacg tacttcggca ctgagcgcag cactggtgcg tccggttaat tcactgacta
    60901 ccgagaccgg ttgagatttg actgtaacaa cgccgacttc tgtttctgac ggggatgaat
    60961 tgttactttg cgtattatca caaccagata agagcagcat tgccactaca gatgttgcta
    61021 tatacttcat tattttctcc agggtgagaa ataccctcct cattaaaatt aatgaggata
    61081 tgtcagggat attcagttat aacgaacccg catcgcgaaa tgaatttcgc aatgaaataa
    61141 gggggattaa tatcccccgc tattggttaa ttctgttctt atttatttat caacatacta
    61201 acaatatcct gatatatttc ctgcagatgt tgtggatcgc ttttttgcgg tgttaaacga
    61261 cgataaaccg taccttccat catggtagca aacacttcaa cgcagcaacg aatgtgctcc
    61321 tcgctaagat ggggatgcat tcttttcata tgtgcacaac cgttagcgaa cattctggcg
    61381 tcagcttctt caagcattgc cataacccgc gggttgcgtg tggcttctgc agccacttcc
    61441 agcatcagcg cgtcgtcgtc ctcatttaag gatttacgca aggcaaggac ttccgggaag
    61501 tgatcggtac gcgcgtcaat gtccatttgg gcaatacgaa agtcgatgat acggcgaacc
    61561 atctcttcaa taatggcgtc tttgttggcg aagtaacgat atatctgacc cacgctaagc
    61621 tgagcttcgc tggcaatttg cgacatactg gcggcatgaa acccgcagcg acgaaaacac
    61681 cgacgggcgg cgttaatgat ttcatcctga cgctgtttgt ggcgtatttc cagcttgctg
    61741 gtcatttctg tctcctggcc tatcacttgg tgagaatgat cgttctcatt tttgctgatt
    61801 atacgtttct ccaaagtaat ggaaagtatt tttatcttat tcatggtgca tattctgata
    61861 atgaagagat tgtggagatt ctgtgaagat agttgacaat attaagttgg ggtcaattca
    61921 tcagaaaggc tgctacttgt tgtcgccagt tctgttttca tcatcaaact tatcggagct
    61981 aatacgaata gtttcatagt cagattctat aagatatttc cccaacctac cccttgcagg
    62041 cgtcaagagt agataacgca aaatgctctg taccgcagct tctctccggt acagaaagcg
    62101 caactatagc cacccacagc caggttgatt ataataaccg tttatctgtt cgtactgttt
    62161 actaaaacga cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag
    62221 ctatgggttt tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca
    62281 tcgcctacgg tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc
    62341 agaacgacaa actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg
    62401 ttctgcaatg cgatgttgcc gaagatgcca gcatcgacac catgttcgct gaactgggga
    62461 aagtttggcc gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc
    62521 tggatggtga ctatgttaac gccgttactc gtgaaggctt caaaattgcc cacgacatca
    62581 gctcctacag cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg
    62641 ccctgctgac cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg
    62701 gtctggcaaa agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg
    62761 aaggtgtgcg tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta
    62821 tcaaagactt ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtaccg
    62881 ttactattga agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta
    62941 tctccggtga agtggtccac gttgacggcg gtttcagcat tgctgcaatg aacgaactcg
    63001 aactgaaata atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt
    63061 tatacctttc tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca
    63121 ggataacgat ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa
    63181 aagccactgg tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt
    63241 cccggaagcc gcaaaggtca gcgatccctt tctactcgac gtgatccttg aaaaagaaac
    63301 gctggccccc ttcctttcat ggctggaccc tgcgcgtgtt cttgcagtgg agttgttccc
    63361 tgaccagctt accgtgaccc gttcacagac cttcaccgct tatgaacgct tgtcgacggc
    63421 cctgacggtt gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact
    63481 tacgccgctc cccgggcctg attccaccag ggaaagtaat catcggttgg cacaaatcac
    63541 gcaatatgcc cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca
    63601 tctgaatgac gtcggtctta ctgcctggga ctgtgtgatc attaaccaaa tcattggttt
    63661 tattggcttt caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg
    63721 ctggttaccc gggctggaga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc
    63781 attacgctgg cgaagcagct atgaggtgga aaaactacct gaagaacaca caaaaagttc
    63841 aactgcagaa ctttgccaac tggccgaaat actctctctc caccctattt cactttccct
    63901 tctcgaaagg ttgttaaaca gcacacgggt taatacacag ccggataatc agcttgcggc
    63961 gttgttatgc gcgcggataa atggcagtcc tgcttgtttt gccgcctgta tggattcatc
    64021 aaatgaatat aaaaaaatca gcccccttct gcgcaagggc gaaaatgaaa ttaaccaatg
    64081 ggctgaccgt cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc
    64141 acccgatcgc tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac
    64201 gcagattatt aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat
    64261 cgcgttgggt gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc
    64321 cgctctaatg gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc
    64381 ttaaacagca actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa
    64441 aaggttttgg cttcctggaa gtcgacgcgc aaaaaagtta tttcattccg ccgccgcaga
    64501 tgaaaaaagt catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg
    64561 aatccgcaga gccagaagaa ctggttgaac cgtttctgac tcgtttcgtg ggtaaagttc
    64621 agggcaaaaa tgaccgtctg gccatcgttc ctgatcatcc actcttaaaa gacgccattc
    64681 cttgccgcgc agcccgtggt ctgaaccacg agtttaaaga aggcgactgg gcagttgccg
    64741 aaatgcgccg tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca
    64801 tcacttttgg tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg
    64861 aaaaagaagc accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtaaag
    64921 atctgaccgc gctggatttt gtcaccatcg acagtgccag cacagaagat atggatgacg
    64981 cccttttcgc taaggcgttg ccggatgaca aacttcagct gattgtggcg attgccgatc
    65041 caaccgcgtg gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca
    65101 ccaactatct gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct
    65161 gctcactgcg cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg
    65221 atggcaccat tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc
    65281 tggtgtatga ccaggtttct gactggctgg agaataccgg tgactggcag cctgaaagtg
    65341 aagcaattgc cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc
    65401 gtcataacca cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa
    65461 aaggtgaagt gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag
    65521 aagcgatgat tgccgctaac atttgtgcag ctcgcgtact gcgcgataag ctcggttttg
    65581 gtatctataa cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc
    65641 tgaaaacgca cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca
    65701 aactgcgtcg tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct
    65761 tccagtcatt tgctgaaatt agcactgaac ccggtcctca ctttggcctc ggtctggaag
    65821 catacgccac ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc
    65881 tgaaagcggt tatcaaaggc gaaactgcga cgcgtccaca ggatgaaatc actgtccaaa
    65941 tggccgagcg tcgccgtctc aatcggatgg cagaacgtga tgttggtgac tggttatacg
    66001 cacgcttcct gaaagacaaa gccgggaccg acacccgttt cgcagcggaa attgtcgata
    66061 tcagccgtgg cggcatgcgt gttcgtttgg ttgataacgg cgctatcgcc tttattcctg
    66121 cacctttctt acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcaccgtac
    66181 aaattaaagg tgaaacggtt tataaagtaa ctgacgttat tgacgtcacc attgccgaag
    66241 tccgcatgga aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc
    66301 attccttatg aatgggccgt ttatttcccc gctctacctt catcatatcc cggcagtttt
    66361 ttaactgact ttcgtttgaa aactggcgtt tttccaaaac tagattacaa ataaatatat
    66421 aaaaaaaaca atgtgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt
    66481 taaccgcgga ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca
    66541 caacgttgta caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg
    66601 atattttgcg cttttctacc accgaaacca cagaacctga acgtacgttg cagttatctg
    66661 ccgaacaggc tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc
    66721 tgaccgtcga tgagagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg
    66781 aatgggctgg caacgcgtct gcatggcatg acacaccagc agttgctcgt gatttatcac
    66841 acggactttc ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg
    66901 acagccgggg gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaacg
    66961 aacacgacgt cattggccaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg
    67021 catccaggcg caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt
    67081 ggataccaac acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg
    67141 gcagtggcaa aaacgagatt tttttaatct gttccggcac cgacattacc gaagagcgcc
    67201 gcgctcagga acgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc
    67261 gtaacgcaat gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg
    67321 gggtcgtgta tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttat
    67381 ttggtgacca gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc
    67441 aggtgttggc gcgtccaggt ggcgatgagt ttctggtact ggcatccaac acctcacaaa
    67501 gcgcgctgga agcaatggca tcacgaattt tgacccgctt acggctcccc tttcgcattg
    67561 gtttaattga agtttatacc agctgttcag taggtattgc actctctccc gaacatggtt
    67621 cggacagcgc ggctattatt cgtcacgccg acacagcaat gtacacagca aaggaaggcg
    67681 gacgaggaca attttgcgtt tttaccccag aaatgaatca acgggtattt gaatatctct
    67741 ggctggatac caacttgcgt aaagcactgg aaaacgatca gttggttatt cactatcaac
    67801 ccaaaatcac ctggcgtggc gaagtgcgca gtctggaagc actagtacgt tggcagtcac
    67861 ctgaacgtgg gttgattcca ccgttggact tcatttccta cgcagaagag tcagggctaa
    67921 ttgtgccttt aggccgttgg gtgattctcg atgtcgtacg ccaggtggcg aagtggcggg
    67981 ataaaggcat taacctgcga gtggcggtaa acatttctgc acgtcagctc gccgatcaaa
    68041 ccattttcac cgccctgaaa caggttctcc aggaactcaa ttttgaatac tgccctattg
    68101 atgttgaatt gacagagagt tgtctgattg agaatgatga actggcactg tctgttattc
    68161 aacaatttag ccaactaggt gcgcaagtgc atctggatga ttttggtacc gcctactctt
    68221 cactttcgca actggcgcgc tttccgatcg atgccatcaa acttgaccag gtttttgttc
    68281 gagatattca caaacaacct gtctcgcagt cactggttcg ggcaatcgtc gctgtggccc
    68341 aggcattgaa tcttcaggtg atcgccgaag gtgtagagag tgcaaaggaa gatgcttttt
    68401 taaccaagaa tgggatcaat gagcggcaag gatttttgtt tgccaaaccg atgcccgccg
    68461 tcgccttcga acgctggtat aaacgctatc tgaagcgcgc ataagtagat gaatatatat
    68521 acttagcgct atattccgtc attatatatc gtattcccgg atgataatta gccgttgctg
    68581 ttaaacactt ctcttttagc ggcaattttt gcattattca ttttccccct tttgctataa
    68641 ggatgtcctt gatgtctgaa tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt
    68701 tagatatcct tgtggcagac gattaccact ctgcgatcca taatctcgag atacttaaag
    68761 cagaactctt gcgccaggtt gctgaatcta cgccagacat cccgaaggca ccgtgggaga
    68821 tttaacaatc gtgccttcag gttttcctat ttgaggatgc ccggcgcgtc agtatttttt
    68881 tatttagtat tataacgtta taagaattac agcgatgaat tcccgacaac aaactattct
    68941 acagatggtc attgaccagg gtcaggttag cgtaaccgat ctggcaaaag ccactggagt
    69001 ttctgaagtt accattcgcc aggatctcaa caccctcgaa aaactgagtt acctccgccg
    69061 tgcacatggc tttgcagttt cgcttgatag tgatgacgtc gaaacccgta tgatgagcaa
    69121 ctatacgctg aagcgtgaac tcgccgagtt tgccgcgtca ctggttcaac cgggcgaaac
    69181 catctttatc gaaaatggca gcagcaatgc cctgctcgcc cggactctgg gggagcagaa
    69241 gaaaaatgtc actatcatca cggtcagcag ctacatcgcg catttgctga aagacgcgcc
    69301 ttgtgaagtt attttgctcg gtggcgtgta ccagaaaaaa agcgaaagta tggttggccc
    69361 gttgacacgc cagtgcatcc aacaggtaca tttcagcaaa gcatttattg gtattgatgg
    69421 ctggcaacct gaaactggat tcaccggtcg cgatatgatg cgtaccgatg tggtgaatgc
    69481 cgtgctggaa aaagagtgcg aagcgatagt cctgactgac agctcgaaat ttggtgctgt
    69541 acattcatac tccatcggtc ccgttgagcg attcaatcgc gtgattaccg attcgaaaat
    69601 acgcgccagc gatctgatgc atcttgagca cagtaaactc accgttcacg tcgttgacat
    69661 ttaaccattt cccttctcta cggatgattt gcagtttgac aaatcatccg ctctaagatg
    69721 attcctggtt gataattaag actatttacc tgttattaac actctcaaga tataaaatta
    69781 ttatcagcga tataacagga agtcattatc acctgcgtga tataaccctg cgcgcgagcg
    69841 gatttcacgg aataatttca ccaggcttat tcttagctat tatagttata gagagcttac
    69901 ttccgtgaat cataaattca ggagagagta ttatgtttgt aacgagcaaa aaaatgaccg
    69961 cggctgttct ggcaattacc ctggcaatgt ctctgagtgc ctgttctaac tggtctaaac
    70021 gggaccgcaa caccgcaatc ggcgcgggtg caggggcatt aggcggtgca gtactgaccg
    70081 atggcagtac gttgggtaca ttaggtggcg cagctgtcgg tggtgttatt ggtcaccagg
    70141 tcggtaaata atcgtaaata aatgtcgatt atcgactcgt aattataata cgcttgattc
    70201 tgttcagtac gaaataactg aataatacac acagtaaaat attcgaaagc cacggatata
    70261 tccgtggctt tttatgttaa ccgcctgcga gttttacctt catccctttc gcttcaagta
    70321 gtgattttaa taaatcacgc ttatcgccct ggatttcaat aactccatct ttaactgctc
    70381 cgccgcagcc gcattttttc ttcagttccg ctgcaagttt tgtcagttcg gcatcatcga
    70441 gatcgatacc ggtaatcagg caaacgccct tacctttacg tccactggtc tgacgctgaa
    70501 tacgcaccac accatcgcct ttagagcgta ctggggccgc tttgggctca tcaatacgcc
    70561 cggtttctgt tgagtagacc agacggctgt tggaatcact catcatgcac tccgctgtaa
    70621 agaggcgttg atcgctttca gcgtctgcgc tggatctacc gattgcgtta ccgggcgacc
    70681 aatcaccata taatcaacac cagccgccaa cgcctgttct ggcgtcataa tgcggcgctg
    70741 gtcaccagct tcactcccct gcggacgaat gcccggcgta accagtttga actcctgacc
    70801 gaatacctgt ttaaagcgca cagcttcctg agcagaacac accacaccat caaggccaca
    70861 tttttgcgtc agtgctgcca gacgttctgc ataatctgca ggtgacagtg tcacgccaag
    70921 atcggccagg tcgctggctt ccatgctggt caacactgtc acagcaatca aaagcggtgc
    70981 atctttgcca aacggaacca gtgcctcacg cgctgcggtc atcatacgcg ccccaccaga
    71041 ggcatgaaca ttcaccatcc acacgcctaa gtcagctgca gcagcgacag cgtgcgctgc
    71101 agtgttgggg atatcgtgga atttcaggtc aagaaagata tcaaaaccac gctgttgaag
    71161 ttcgcgcaca aactgtggcc caaacaatgt aaacatctct ttgccgacct tcagacgaca
    71221 atcgcgtggg tcgatcttgt cgacaaaggc cagcgcggca tcacgattat gataatcaag
    71281 ggcaacaacc acaggagaat tcgtaacagc gcgggaagaa gatgaagcag ttaacgtcat
    71341 gaccagacct tcttgatgat gggcaccgga agtggcgcga aacaggtaaa cggcgagcat
    71401 tctacccgtg gaaataaaaa attaacagtt gcgatttctc ccctggcagg ctttttctgc
    71461 gccgccgttg ccggagcaca atgggacata aataactagt atgttgtaac taaagtcggt
    71521 ttttttaaaa aattacaggc catcaagacc gcgaatcggt ttaatagttg accaggcacg
    71581 acaagacgga caatgccagt agagggtgta tgcggtaaaa ccacatttct ggcagcgata
    71641 acgaggctta ctacgtacct tctcgccaac catgtcacgc agcaccatca ggctctcttt
    71701 ggcacgccct tcttccgctt catttaagtg gtaatccatt aacttatgga acacacgcat
    71761 ggtcggatga cgctgaagct ggcgcgtaat atagacctgt gcggcctcac taccgtcgcg
    71821 cgcttcgatg atatcagcaa gcatcaattc agcatcggca ccggtgttct cttccaccgc
    71881 gcgctgcagg aattctgccc attcggcagt tttacccaac tgctgatagc aggtttgcag
    71941 catttccagc gtttcgctga ccagttctct gtcctgggat atcacgcgtt gcaaactttc
    72001 gacggctttg gcgtattctc ctttcgccat aaacacgcgt cccatcatga tggatacgcg
    72061 ggcgctgttt ttatctgccg ccgccccttt ttttagcaag gtcatggcac gatcgagatc
    72121 gtcgctggcc atatgctgca gggctaactc acagtagaaa tgggcaattt cgacgcgctg
    72181 tttatcttta cccagcttca ccaggcgttc ggcaacatca attgctttct gccactcact
    72241 ggtagcctgg tagatttgta gcaactgttg cagcgcgcca atgcggaagt cagtttcatc
    72301 ggtcagctga ttgaacatgt cttccgcgcg gtcatataac ccggcggcca tgtaatcacg
    72361 ccccagttgt tgaatcgcca acagacgctg ttcataggtc agcgaggcgc tttccattag
    72421 ggtctgatgg atgcgaatag cgcgatcaac ttcgccacgc gaacggaaca ggtttccgag
    72481 cgtaaggtgg gcttcaacgg tgcctgtatc ctctttaagc atatcgagaa acaggtctac
    72541 cgctttatcc tgttgattac taagcaggaa gttaaccccc gctacgtaat cacgcgacaa
    72601 gcggttggct tcatcttgct tgttttgttg cgcacttctg cggcccatat accagccata
    72661 ggcagcggct acaggcaaaa gcagaaacaa caactccagc atagaaagtt attccttcgc
    72721 cgctgacgag tgctgcacta cagccacgtc agtcgcgggt gaaagctggt tttccagtcg
    72781 ctttatttta cgttcagcgc gcaccaggga aacacgaact cgcagccaga acaggccaca
    72841 aatcaaccaa ccgatagcaa aacccgcagc aaacaatacc gccagcaatg tggaaatacg
    72901 gtactcccct tgcgctaaca gataattaaa cgtcacctgt tgatcgttct gcgcacccaa
    72961 cgtgaccgaa atcacgaaga tcgctaacac cagtaagaaa atgagtaaat atttcacatt
    73021 acttcccgtt atgtggttag agcgaataaa ttatggtcaa cttaccgcaa tagcccttta
    73081 aagtaccatt ttcgcgccgt ctgcgaaatg gcatccacgc gcattgttga tgatttaagg
    73141 gctaaattcg gaaaatcaac cagcgttaac tttcttgttc tcgttgcgct atttcgcgat
    73201 tttcttccgc aggtggtgtt aatggcccgc aaattcgttg cgcaagccac gttgccaccg
    73261 ccaccagtgc ccacgaaatc aacgtagcta ccaccagatc gcgtggccaa tgcatcccga
    73321 gcagcaggcg gcttcccatg actcccgttg cccagaccag caagatagca atggttaacg
    73381 ttcgccgacg cggccacagc aaaccaacgg ccagcagtgc ccaactggca gcaaacatcg
    73441 tgtgaccgga aggaaaggca aaccccgtct ctttctgcca gtgtgaacgc aaatattgtg
    73501 gaatattttt ctcttcagcc aactgttctt tcactagatt tccgcgttct gctcgcttta
    73561 aagtgtagaa ctcatcaacc ggaatatgat gtgttttttc cagccagata acaaaaggtc
    73621 gtggttcctg gactttgtct ttgatccagg atttaacgcc ttgtcccaca aggattgcgg
    73681 ccgccagaat ggcaaataat acaaaggcag ccttaatgcg aaaacgcaga caccagagaa
    73741 accagccgaa taaaatcaaa tgtgtaatga cgccccaggg ctgggtgaca gtttcagtaa
    73801 cccaaaaagc cgcttttagt agccaacttt gttctccagg ttgccaacgc cagccagaaa
    73861 tccatacggc tactggcata acaagcaata gtgcagctcc cactgcggta cgtcttgcaa
    73921 tcgaacgcat ggcctctcct ttttgataag tcccacaatc ataactgaaa atgccagttc
    73981 caggaaaaat tgacagattt gtgccattcc gtgaacgatc gacgcgtcgt gattaggtga
    74041 accccttctc gttatggcaa aataagccaa tacagaacca gcattatctg gagaatttca
    74101 tgcagcttaa acgtgtggca gaagccaaac tgccaacccc atggggcgat ttcctgatgg
    74161 tgggatttga agaactggca accggacacg atcatgtcgc gctagtctat ggcgatattt
    74221 ccgggcatac cccggtactt gcgcgcgtcc attccgaatg tctgaccggt gacgccctgt
    74281 tcagcttgcg ctgcgattgt ggcttccagc tcgaagcggc attgacgcaa attgccgagg
    74341 aaggccgtgg tattttgctg tatcaccgtc aggaaggtcg taacattggt ctgctgaata
    74401 aaatccgcgc ttacgcactg caggatcaag gttacgatac tgtagaggct aaccaccagt
    74461 taggcttcgc cgctgatgag cgcgacttca ctctttgcgc tgatatgttc aaactccttg
    74521 gcgtcaatga agtccgcttg ttaaccaata acccgaaaaa agtcgaaatt ctgaccgaag
    74581 cggggattaa tattgttgaa cgcgtaccat tgattgtagg tcgtaacccc aataacgaac
    74641 attatctcga taccaaagcc gagaaaatgg gccatttgct gaacaaataa ccctcttgca
    74701 ttgtgtaatt aatttgcttg ccggaagcaa aataaccggc aagcaaatac ttgttacttc
    74761 aacatattac gaatgacata atgcaaaatg ccgtcgttct ggtagtaagt caactccgtc
    74821 gcggtgtcga tacgacaacg gcagggtacg acttcctggc taccatccgc gcgcgtaagc
    74881 gtcaccggaa ccgtcgcgcc gggttgtaga ttttgcagat cgccaatatc aatcttctct
    74941 tccccggtta gccctaacgt tttacgcgtt acgccttgcg gaaattccag cggcaggatg
    75001 cccatgccaa ttaaattcga acggtgaatt cgttcaaacg attcggcaat caccacacga
    75061 ataccaagca gacgcggacc ttttgccgcc cagtcacggc tggagcctga tccatactct
    75121 ttcccggcaa tcaccgccag cggcgtttgc tcctgcttat agcgcatcgc agcatcataa
    75181 atagagacta cgtcgctgtc aggtaaatgc cgcgtcatcc ccccttcaac gccaggcacc
    75241 atttcattac ggatgcgaat attggcgaag gtgccgcgca tcatcacttc atggttacca
    75301 cgccgcgaac cgtaggagtt aaagtctttt cgctcaacac cccgaccttg taggtatcga
    75361 cccgctgggc tgtcgggctt aatactgccc gccggagaga tatggtcagt ggtgactgaa
    75421 tcccccagca ttgcgaggat ccgcgcaccg tgaatatctt ccacaggtgc tggcgttgcc
    75481 tgcatttcat caaagaaagg cgataaacga atataggttg aatcctcctg ccatccgtag
    75541 gtatcggatc gtgcgacgtt aatttctttc cactctgcag tgccttcaaa gacttctgcg
    75601 tactctttgc ggaacatttc tgtggagact tgttctaccg cacgggcaat ttcttgtgcc
    75661 gatggccaga tatctttcag atatattgga tcgcctttac gatcatgacc aataggctca
    75721 gaagccaggt tgatattcat atttcccgcc agcgcatagg caaccaccag cggcggcgag
    75781 gccagccagt tagttttaac cagcggatgg atacggcctt caaagttacg gttgccggac
    75841 agcactgcac cgacggttaa atcgcctttt ttgattgccg tttcgatagg atcgggcagc
    75901 ggcccagagt taccaataca ggtggtacaa ccgtatccca caaggttaaa ccccagttcg
    75961 tcgagatacg gtgtcagttt cgcttttgcc agatagtcag aaacgacttt cgaacccggt
    76021 gccagcgacg ctttgaccca cggttgccgc ttgaggccaa gagttacggc ttttttcgcc
    76081 agcaagcctg cggccatcag cacacttggg ttagaagtgt tggtgcacga ggttatcgca
    76141 gcaatgacca cagcgccatc aggtaactga tactgatgtc cgttcataac ataatcgacc
    76201 ggctggcgat ctttatgcgt ggcattcact tccagttcgt tactggcggc aaatgctttt
    76261 ggtacatcgg gcagtgcaac gcgatcctgt gggcgtttag gccctgccag gctcgcttca
    76321 acgtcattca tatccagatc taacgtactg gtaaaaatcg gttcatcgcc cgggttacgc
    76381 cacatgccct gcgctttggc atatttttcg accaactcga cctgatcttc gctgcgcccg
    76441 cttaaacgca tgtaatcgag ggttacagca tcgattggga agaagccaca ggtggcacca
    76501 tattctggcg acatattggc aatggtggcg cgatccgcca acggtagtga atccagacca
    76561 tcaccataaa attcgacgaa tttccccacc acgccatgtt tgcgcagcat ttgggtaaca
    76621 gtgagaacca ggtctgtggc ggtaatacct tcacgtaatt ttccggtaag tttaaagccc
    76681 actacgtccg ggataagcat ggaaaccggc tggcctaaca ttgcggcttc tgcttcgatg
    76741 ccacccacgc cccaccccag cacgccaagg ccgttgatca tagtggtgtg cgagtcagta
    76801 ccaacgagtg tatccggata agcaatccat tcaccgtcct gcaattcgct ccacactgct
    76861 ttgccgagat attcgaggtt aacctgatgg caaatgcctg tgcctggcgg cacgacgcta
    76921 aaccgactga acgcttgctt tccccatttc aggaacacat aacgttcgtg gttgcgctcc
    76981 atttccaggc gtacgttttc ttcaaatgcc tcatcatcac caaaacgatc gacggtcacc
    77041 gagtggtcaa tgaccaggtc gaccggtgag agcgggttaa cctttgcagt atcgccaccg
    77101 aggcgtttaa ccgcttcgcg cattgccgcc agatcaacaa cggcaggtac gccggtaaag
    77161 tcctgcatca gcacccttgc cgggcggtag gcaatttcac ggtcagcatg ggcatttttc
    77221 agccatcccg ccagcgcgtg gatatcctct tcggtaaccg agttaccatc ctgccagcgc
    77281 agcagatttt cgagcaaaac tttgagtgac ttgggtagac gggtgatatc gcccagtgat
    77341 ttggcagcaa gcggcaggct gtagtagtgg taagttttat ctttggcctg caacgcgtcc
    77401 ttactggctt ctcgtagggt tgacgacata gctcctcctt aatgacaggg ttgcgaagcc
    77461 ctgattctct tcagggtgaa tattaaagat aacacaaaca tcgcgtaacg atttgataac
    77521 aacccaaatt gataaaagag gagaaacctt gtggcaaagc aaatgacaac cccgccgcag
    77581 cggggtcaag gatcagtgag taatgatcca gaggagttcg gcccagaaga gcatcgacag
    77641 agaaaatgca cccatccagg accaatattt catatttgtg ttgatcatag gctacaccct
    77701 taacggttat tactttgtgt tgagataact caacctgtta ttgctgcggt gtattaaaag
    77761 ccctttaatt cattaaaaat gggtaataat aatgatgata attattgttt cggctgatca
    77821 tcttcagcaa acagttctat aaaggcttgc tgctgcgatg ttagttgcca gtcgtctgga
    77881 ataacgacgg aacattcccg tttcgtttcg ggttcattgt cactatgctg gcataatggt
    77941 gacgactcca gttgctcctg accaaccata gttatcccca cactttccaa atcagcagtg
    78001 cgacaaccac ccagaaaagt gcagaaccaa gaaaaaccgc cagccaggct ttacgtttta
    78061 attcaggatc gcggcggggt tcctgatttc cagaaggcat tgctaacctc atataatcgc
    78121 catcacttat cagcaagacg ttgaacgatt ggtgtaatta atcatacgat gagacaaatc
    78181 ctaaagaaac tttagtcaaa aatccagcga atttacgaat cgtctccagt gaataattca
    78241 atcttttgac ctgaaataga tatttgaaaa ctggaatttg cgaggcgggt aattagacac
    78301 tactttacag gtaatttacc ctataaatat gagggataaa aatattgtgc acggtataca
    78361 aataagtaac aaaagagaat atattccggc accttcgcta cataaaaggt gccgaaaata
    78421 acgcaagaaa ttatttttcc ggcagtttta tatctttaaa catgacctca atttcttcat
    78481 tggagcgcaa tgcgacagcc gcatcaacga catcacgcgt taaatgcggt gcaaaacgct
    78541 gaatgaaatc atacatataa ctgcgcaaaa aagtactacg gcgaaaacca attttggttg
    78601 tactgtggct gaagatatcg tgagcatcaa cacgcacaag gtccggatcg gcgaccggat
    78661 ccaccgccat gctggcaatg acccctaccc ccagccctaa ccggacgtaa gttttaatga
    78721 cgtcagcatc cgttgccgtg aaaacgatac gcggcgttaa ccctgcgcga ttaaaggcag
    78781 tatccagttc tgaacgtcca gtaaagccga aggtatatgt caccaacgga tattgcgcca
    78841 gttcttcaat ggtaatggct tttttgcctg ccagcgggtg atccggagtg actacaatag
    78901 cccgattcca gtggtagcac ggtaacatca ctaaatcttc atacagatgc agcgcttctg
    78961 tggcgatagc gaaatctgca ttacctttag agacggcatc agcaatttgt gtcggcgaac
    79021 cctggtgcat atgcaaagaa acgcggggat aacgctcaat aaagcctttg atgacgtttg
    79081 gtaacgcgta gcgtgcctgg gtatgcgtgg tggcgatata cagcgagcct ttatccggcc
    79141 aggtgtgctc accggcaacc gattttatgg catcaacttt cgacaggact tcgcgagcga
    79201 tacgaattat ttcttgtcct gctggcgtta cctgcgtcag gtgcttgccg cttcgggaaa
    79261 aaatttgaat gcctagctcg tcttccagca ttctgacttg tttactgatc ccgggttgtg
    79321 atgtgtaaag tccttccgct gttgatgaga cattcaggtt atgattgacc acctcaacaa
    79381 tatagcgaag ttgttgtaat ttcatgttaa accatccact tagacgccat cagaacatcg
    79441 ttttttcgtc acacctggcg ggtgactaat acgatttaat aataaaaaag gtgctaacta
    79501 taaccactat atcatttata gccttagtgt ataggtgtga agaaaaaata atatgcaata
    79561 aaaaagggcc gctttcgcga ccctttgttt ataaaaacct gacagaatta aaggttattt
    79621 tttcccttca acccatttgc catcaacata aaatgctgac cagccagtcg cctttccgtc
    79681 tttttccgaa gagacatatt gctgtttggt tttacggcta aagcgaacca tagtcttatt
    79741 accttccgga tcctgctgcg gcgcatcggc cagataacgc agtttttccg gcagacgatc
    79801 gcggaagcga tacagctctt ccaccagcgg cgcacgcgtt tcacgcgatt tcgggaaggt
    79861 attggccgcc aggaacacac cggcagcacc gtcacgcagc acgaaatagg catctgattt
    79921 ttcgcacggc agctccggta atggtaccgg atcttctttc ggtggagcca cttcgccgtt
    79981 acgtaaaatc ttacgcgtgt ttttacactc ttcgttggtg caggccatat atttaccgaa
    80041 acgccccatt ttcaggtgca tttcagaacc acatttttca cactcaacga tcgggccgtc
    80101 ataaccttta atgcggaatt cgccctcttc gatctcgtaa ccgtcgcagg ttgggttatt
    80161 accacagaca tgcaacttac gtttcggatc gatgagatag ctgtccatcg ccgtgccgca
    80221 tttggggcaa cgacgttttg cgcgcagcgc gttggtttca gcgtcttcgc cttccagcac
    80281 gttcagcact tcgttttccg gcaccaggtt aatggttgtt ttgcaacgct ctttcggcgg
    80341 cagcgcatag ccagaacagc caaggaatac cccggtgctc gctgtgcgaa tccccatttt
    80401 gcgaccacag gtcgggcagt cgatgctggt cagaaccatc tggttcggac gcataccccc
    80461 ctcttccgga tctttttcag ctttatctaa ctgctgagtg aaatccgaga agaagtgatc
    80521 cagtacagct ttccactctg cttcgtgatt tgccacctgg tcaaggctgt tttccatctg
    80581 cgcggtgaag tcgtagttca ttaactcgcg gaaattctct tccaggcgat cggtgacgat
    80641 ttcgcccatt ttttccgcat agaaacgacg attttctact cgcacatagc cacgatcctg
    80701 aatggtcgaa atgatcgacg cataggtaga cggacgaccg ataccacgtt tttccagttc
    80761 tttaaccagc gatgcttcac tgaaacgggc tggcggcttg gtaaagtgct gggctggtgt
    80821 cagttcaacg agcgtcagag catcgccttt atcgactgca ggtaagatac gatcttcatc
    80881 gcctttacgc agtgcaggca tcactttcgt ccagccatca aagcgcaaaa tacgaccgcg
    80941 tgctttcagg cggaaatcgc ccgcaccaac ggtcagcgtc gtggagtcat atttcgctgg
    81001 ggtcatctgg caggcaacga actgacgcca gattaactgg tacagtttct gcgcatctgc
    81061 ttccatatcc ttcagcgatt ccgccatcac attgacgtca gaaggacgaa tcgcttcgtg
    81121 cgcttcctgt gagttttctt tgctggcgta ctgattcgga ctttccggca gatatttctt
    81181 accaaaatta tcgctgatat aaccgcgaac catatttacc gcgtcctgac tcaggttagt
    81241 ggagtcggta cgcatgtaag tgatatagcc tgcttcatac aaacgctgcg ccatcatcat
    81301 ggtttttttc acgccaaatc caagacgggt gctggcagct tgttgcagcg tagaggtaat
    81361 aaaaggagcg ccaggtttac tggttgtcgg tttgtcttca cgttccagca cgctgtagcg
    81421 cgctttttcc agcagactta ccgcagcctg agtttgttct ttgttgaccg gacggaacgg
    81481 tttgtcgttc tgatgagtca cctgcaacgc caacgcttca ccagatggcg tggtcgtgct
    81541 ggcatcgact tcccagaact cttccggcac gaacgcttta atttcacgct cacgctcgac
    81601 caccaggcgg actgccaccg actgcacacg accggcagac agaccacgag cgatcttttt
    81661 ccatagcagc ggcgaaacca tataccccac cacgcggtcc ataaagcgac gcgcctgctg
    81721 ggcattaaca cgatcaatat tcagctcacc cggtttgtta aatgcctggc ggatcgcgtt
    81781 tttagtaatt tcgttaaaca ccactcggct atagcgcgca tcatcaccac caatcacttc
    81841 ccgcaggtgc catgcaatgg cttccccttc gcggtcaagg tcggttgcga gatagatgtg
    81901 gtcggctttt tcagccagtt gtttcagttc agagacgacc ttctctttac caggcaacac
    81961 ttcatagtgc gcctcccaat tgtgccacgg gtcaaccccc atacggttga cgagagcgcc
    82021 acgttcatcc tttttaggct ttttagccgt cttggtggag gtagagtcgg cactcttttt
    82081 ggcagctgag ccactggtcg gcaaatcgcg gatgtgaccg acgctggatt tcaccacgta
    82141 gtcactaccc agatacttgt tgatcgtttt ggcttttgcc ggggactcaa cgatgacaag
    82201 agctttaccc atattcacct tacctaattt gattcttcca ggaatgcgtc gcacgttgat
    82261 aaaccttcca ctggcaacga gttactgata tggactcgct ctctgcggat atcaacccct
    82321 tgtcgtgact ctgttcacga aaattcaggt gactgagtta acaggtcttg gcctacaggc
    82381 gctatagcac gacgaaattt tggtcgaatg tcaagcaaat ctattgccag attgacgaaa
    82441 gcgtcacact gtacctgata aaatccgata cgcaacttta ttagcatgca aaatccaaag
    82501 ttgccagtaa ggcgctcttg ccaccagcgt ggtatatttg tctgcaagcc atgcctggcg
    82561 tagactaatg tccttgttta aggagtaaat aatgaacaaa gagacacaac ccatcgaccg
    82621 cgaaacgctg ttgaaagagg ccaacaaaat cattcgtgag catgaggata cgctcgcagg
    82681 gattgaagcc accggcgtca cacagcgtaa tggcgtgctg gtatttaccg gcgactactt
    82741 tttagatgaa caaggattgc caacagccaa aagcaccgcg gtgtttaata tgttcaaaca
    82801 cctggcccat gtgctttcag aaaaatatca tctggttgat taaagcaaaa cgcgaggtct
    82861 taccctcgcg tttgtctttt acatcaatgg cttttgccca cgctgccacc agcgtagcaa
    82921 taatcgatcg gcgctctctg ccgcgctgcc ggtgaatcgg tcaatgagtc gtttacgctg
    82981 catatagcgt acattgacca cttctcggcc ttccatcagg ctaagaataa cttcatcact
    83041 ggtgttgatt tcatcaacca ggcccttctc taccgcctgt tgtccgtacc agtgttcacc
    83101 cgttgccacc tgttcaatat ccagagacgg acgcatacgc ttcacaaaat ctttaaataa
    83161 ctgatgcgtt tcgttcagct cttcgcggaa tttctcccgc ccttcttcgg tattttcacc
    83221 cagcaaggtc agcgtacgct tatactgccc ggcggtgtgc agttcgatat caatatcttt
    83281 gcttttcagg aagcggttaa agttgggcat ttgcgccacc accccaatgg aacccacaat
    83341 agcaaacggt gcggaaacaa ttttgtccgc cacacaggcc atcatgtaac caccgctggc
    83401 agcaactttg tctaccgtaa cagttaatgg aatgttttta tcacgcagac gctgcagctg
    83461 cgaagccgcc aacccgtaac catgcaccat gccaccaggg ctttccagac ggagcacaac
    83521 ctgatcctgc ggtttgaatg ctgcaagtac cgccgttatc tcttcacgta gcgagttcac
    83581 ttcatgggcg tccatgctgc ctttaaagtc cagcacccag acgcggggtt tactgtcagt
    83641 tgacacctcg cccagtttgg ctttcgcttt tgctgctttc gcttcttgct tgtgcttttt
    83701 cttctgcgct ttgtgccact gtttttgctg atgtgagtcc atcagcgccg cggccagttc
    83761 ttctttcatc tccttatact gttcgctgag attgttgacc cgtaactcgc cacgctggcg
    83821 tttattacgt tgagcaacat tgacaataat ggcggcaatc gccgcaatcg ctagcacaac
    83881 ggtaacgatt ttcgccaaaa acaaaccata ttcagacaac aattccacga gtccccacct
    83941 tggttaaaca acgctttggc tcacagttta caacaggcat gttaagccgt ctcgcagaga
    84001 tgttattgca gcgaaagtat gacaaagtat ccattttgcg gtgttatcgc ccgaggctga
    84061 ttgaaatgcc cttctgtttc aggcataaag ccccaaagtc ataaagtaca ctggcagcgc
    84121 gacagacaac gtcgttgagg agtagccatg cattaccagc caaaacaaga tttactcaat
    84181 gatcgcatta tcctggtgac gggagccagt gatggtattg gtcgtgaagc cgcgatgacg
    84241 tatgcacgct atggtgcgac agtgattctg ttgggccgta atgaagaaaa attacgtcag
    84301 gtagccagcc acataaacga agaaactggg cgtcagccac agtggtttat tctcgatttg
    84361 ctgacctgca ggtccgaaga ttgccaacaa ctggcacagc gcattgccgt taattatccg
    84421 cgtctggatg gtgttttgca taatgccgga ttactcggcg atgtttgtcc aatgagcgaa
    84481 caaaatccac aggtctggca ggacgtcata caggtcaacg ttaatgccac ctttatgctc
    84541 acccaggcac tacttccttt attactcaaa tcggacgccg gttcactggt ctttacttca
    84601 tcaagcgttg gacgtcaggg acgagccaac tggggtgcat atgcagcgtc gaaatttgcc
    84661 accgaaggga tgatgcaggt actggccgat gaatatcagc agcgcctgcg tgtcaactgc
    84721 attaacccag gcggtacgcg caccgcaatg cgtgccagcg ccttcccgac cgaagatcca
    84781 cagaaactta aaacacccgc tgatatcatg ccgctctacc tctggctgat gggcgatgac
    84841 agccgccgta aaaccggcat gacctttgac gcccaaccgg gccgtaaacc aggaatttcc
    84901 caatgagtga tgaacgctac caacagcgtc agcagcgagt gaaagaaaaa gtagatgctc
    84961 gtatggccca ggcccaggat gaacgcggta ttatcatcgt ctttaccggc aatggaaaag
    85021 gcaaaaccac cgcggcattt ggtacggcaa cacgcgcagt tggtcacgga aaaaaagtgg
    85081 gcgtcgtgca gtttattaaa ggcacctggc ctaatggcga acgcaatctg ctggagccac
    85141 atggcgttga gtttcaggtg atggcaacgg gctttacctg ggatacacaa aaccgcgagt
    85201 ctgataccgc cgcctgccgc gaagtctggc aacatgcaaa gcggatgctt gctgattcct
    85261 cactggattt ggttttgctt gatgagctga cttatatggt ggcgtatgac tatttgccac
    85321 tggaagaagt gttgcaggcg ttaaatgaac gtccacatca gcagacggtg attatcacgg
    85381 gtcggggctg tcatcgggat atactcgaac tggcggatac ggtaagtgag ttacgccccg
    85441 tgaaacatgc gtttgatgct ggcgtaaaag cgcagatagg gattgattac taatctgtaa
    85501 aaatgccgga tatgacgtcc ggcatacgtt gattaaccgt tattacgccc accagaacga
    85561 cgtccgccgc tcacctgact gtgacgtttc accgcacgac gaatctgatt cgccttcatg
    85621 cgacgacggt ctttttctac agcgacttta gagctggttt ctggaggcaa ctccaccagt
    85681 tcgcgcagat agttagtctg ggcaagatcc agctccgtcc agccgccgcg tggcagacct
    85741 ttcggcagtg ggatatcacc gtaacgaaca cggatcaggc ggcttacctg cacgccaacc
    85801 gcttcccaca gacgacgaac ttcacggtta cggccttcgg tcagagtcac gttgtaccac
    85861 tggttgatcc cttcgccgcc gctgaacttg atggttttaa aggctgccgg accatcttcc
    85921 agctgcacac cacggcttaa atcacgcagt ttcgcgtcgt caacctgacc aaatacacgc
    85981 acggcatatt cacgttcaac ttcacggctt ggatgcatta aacggttagc cagttcaccg
    86041 tcggtggtga acagcaacag accacaggtg ttaacgtcca gacgacctac ggcaatccag
    86101 cgtgcaccgc gcagctttgg cagacggtca aacaccgttg gacgcccttc cggatcgtta
    86161 cgggtacaca actcaccttc cggtttataa taggccagaa cgcgacaaat ttgttcagcg
    86221 gactcacgta ccgaaatcag gtgaccatcg atacggattt tcaatccagg ggtaacttca
    86281 acgcgatcgc cgagtttagc aattttgcca tcaacactca cacgaccggc ttcaataata
    86341 gattcgattt cacggcgaga accgtggccg gcacgcgcca gcactttctg tagcttttcg
    86401 ctcatagagc ttccttttgg tgtcgccttc ccaggcgtct gatatgcttt tgtgcaacag
    86461 tgagtcagaa cttacatctg actcactgat agtaaagtct gtttgaactc ttcaggcctt
    86521 aagtaaacga ctcgacgttt taactggcct ggctttgagg cgcaacatac tacactatct
    86581 ttatggttaa agcttgtttc gattaacatc agcttaatta ccagccacca ccgcctccgc
    86641 cacccgcagc accaccggaa taacttcgcc ccaataaact ggtattggcc cccagactca
    86701 ggtgcatctg taatgagaac aaaacgccac tacgggcaat ttccggggcg cgaacagccg
    86761 ctgtgagtga tggggtgata cgtgctaccc attctcttcc aaggtttgct gagacagccc
    86821 aacctagcaa cgcgtaatcc agtttccgtg tttccccttt ccgccgacgc ccactgtgtg
    86881 ttgctgcctc tttacgcgcg agatagttaa cgatagcttc accgtgggca taacgttgct
    86941 gcccagtttg ggtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca
    87001 ggaaaacagc gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat
    87061 tgatatagag agaataaaag gccacgcctg aacaaatcag cggtaaaaac aaagtgatga
    87121 ttacacgatt aacgcaaggc ttgtcatcat cgcgggcaaa caaaagctca agggaacaaa
    87181 ataaaaaagg aagaagccag cagatcataa taaacatatc gcccaccaga ctcattcctg
    87241 cccagcctac aggactcagg ttaccgtaaa gaatgaccgc caatattgcc agtccacccc
    87301 attgcaaaaa agtatcaggc cggtaaaacg aagacttttt acgcggcaga taaaatttct
    87361 gcatccgtaa aacatctacc gacgtaaatt gcggcctgcg cttccagaga tataaccatc
    87421 cacaaaccag gaatagacac ggaaaccaaa ttaacaacga tgacgtggag ggtaaaagaa
    87481 gatggctgaa aatattcgtc gtttgtggag ccggggcatt gctaagtaac gagtgaggcc
    87541 aacggtagag taccgtgaaa tcttcacgat aaaacggatc gctggattca attcttccgt
    87601 cctcaaggat gcggccattt cgatatgtgt cgccctcttc tccagtaaag agatcgattt
    87661 cgctaaatgg attacccgcg atatcaggga acttgagtga aaatcgggtc ttatagattt
    87721 caaacggcca gtggttacca gtcacgttcc agattaacag atctgaatct ccctcacgca
    87781 ggaaagcatt tttaacctga taatgaattt gataatgata gtcgccttga ggcaagaaac
    87841 gttgtttatc gcccacgaca atactcatta acccgatatg ccagtcaaga tgccagggtt
    87901 caggaatacc atcacgggtg acgccaacaa tatgatagtt cagcagtgca gcgtccccat
    87961 caggctgcat ccatagtcgt ggtaaatcac gataaaatcc gtgcttaatc tgctgatttc
    88021 gcgcctgaaa atgaatattt tctttgatgt cgagcacccc atccggttgc agagtggctt
    88081 caacatcaaa gagcgagatc ccttcttcat aagaaggcat ctccgtgttt ttcgcataac
    88141 ttaacgaaga gaaaaaaagc ccaactatca acagcaaaat gcagcgaaac ttccctgcca
    88201 tagtcttcca tccgtggaac gcaatttgtt ataagaaagg cttcacatca cctacgcctt
    88261 cacgcaccac gaccggcgta tcatcggtaa gatcaataac cgttgttggt ttctgcccga
    88321 gatagccgcc gtgaataatc aaatcgacct gtttttccag acggtctttg atttcttcgg
    88381 gatcggattc ggtaaattcg ctgcctggca gcattagcga agtggaaagc atcggttcgc
    88441 ccagtgcttc aagcaacgcc tgcgcaatcg ggttagaagg cacacgcata ccgatggttt
    88501 tgcgtttttc ctgcaacaga cggcgtggca cctccttcgt ccccttcagg atgaaggtat
    88561 agttgcccgg cgtgttgttt ttcattaaac gaaacgcaac gttatcgaca aatgaatagg
    88621 tcgagagttc agaaagatcg cgacacatca gggtaaagtt gtgaccgtcc ggcagctggc
    88681 gaatacgaca aatacgctcc atggcgtttt tatcttcaat tttacagccg agcgcatagc
    88741 cggaatcagt tggataaaca atcaccccgc ctttacgcac gatctccacc gcctggttga
    88801 ttagacgttg ctgcgggtta tcaggatgaa tataaaaaaa ctggctcata attccctctc
    88861 tgtggtgttc tgcggctgtt cccatagctg ccagacgcct tcaacgcctg cgggcagcca
    88921 gagtttacga cccagttcga tccacgggca tggctgatga aaatcagatc cttgtgatgc
    88981 ccataaatgg tgctggcggg caagcgtcgc cagctgggta cgttcattgg gcgattgctg
    89041 gcactgcgcg acttccatcg cgtcgccgtc gtgttcggca aaatgcgcaa ccaatctttt
    89101 cagccattta gcactaagat tgtaccgccc aggatgagct agcaccacct taccgccaga
    89161 atgatgaatg acatcaatag cttgttctat tgtacaccac tgtggcggaa cgtatccggt
    89221 tttcccgcgc gccagatact ttttaaagac atccgccatt gaactggctt tgccgcactc
    89281 aacaaggaaa cgcgcaaaat gaccgcgcgt cactgcgccg ccctgcgcca gtcgttgtgc
    89341 gccttccagc gccccaggta tatgcgcttt ttcaagtctt tcggcaattt gctgcgcccg
    89401 ctgattgcgc cgttctgtct gctgcgcgag gaactcacac attagcggat gagtaatatc
    89461 aatattcagc ccgacaatat gaatttcatg attttcccag accgtggaaa tttccacgcc
    89521 gggaataaga ttcagcgcca gtcccgaacg tgaaatttcc tctcttgctg gcgcgattgc
    89581 agctgtggtg tcatggtcgg tgatcgccag ggtgccgacg cgcatctcga ctgcacggtg
    89641 caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta
    89701 aatcactgcg taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg
    89761 ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat caatcatcga
    89821 actagttaac tagtacgcaa gttcacgtaa aaagggtatc gacaatgaaa gcaattttcg
    89881 tactgaaagg ttggtggcgc acttcctgaa acgggcagtg tattcaccat gcgtaaagca
    89941 atcagatacc cagcccgcct aatgagcggg cttttttttg aacaaaatta gagaataaca
    90001 atgcaaacac aaaaaccgac tctcgaactg ctaacctgcg aaggcgctta tcgcgacaat
    90061 cccaccgcgc tttttcacca attgtgtggg gatcgtccgg caacgctgct gctggaatcc
    90121 gcagatatcg acagcaaaga tgatttaaaa agcctgctgc tggtagacag tgcgctgcgc
    90181 attacagctt taggtgacac tgtcacaatc cagtcccttt ccggcaacgg cgaagccctg
    90241 ctgacactac tggataacgc cctgcctgcg ggtgtggaaa atgaacaatt accaaactgc
    90301 cgtgtgctgc gcttcccccc tgttagtcca ctgctggatg aagacgctcg cttatgctcc
    90361 ctttcggttt ttgacgcttt ccgcttattg cagaatctgt tgaatgtacc gaaggaagaa
    90421 cgagaagcca tgttcttcgg cggcctgttc tcttatgacc ttgtggcggg gtttgaagat
    90481 ttaccgcaac tgtcagcgga aaataactgc cctgatttct gtttttatct cgctgaaact
    90541 ctgatggtga ttgaccatca gaaaaaaagc acccgcattc aggccagcct gtttgctccg
    90601 aatgaagaag aaaaacaacg tctcactgct cgcctgaacg atcttcgcca gcaactgacc
    90661 gaaaccgcgc caccgctgcc ggtggtttcc gtgccgcata tgcgttgtga atgtaaccag
    90721 agcgatgaag agttcggtgg cgtagtgcgt ttgttgcaaa aagcgattcg cgccggagaa
    90781 attttccagg tggtgccatc tcgccgtttc tctctgccct gcccgtcacc gctggcggcc
    90841 tattacgtgc tgaaaaagag taatcccagc ccgtacatgt tttttatgca ggataatgat
    90901 ttcaccctgt ttggcgcgtc gccggaaagt tcgctcaagt atgacgccac cagccgccag
    90961 attgagatct acccgattgc cggaacacgt ccacgcggtc gtcgcgccga tggctcgctc
    91021 gacagggacc ttgaaagccg tattgaactg gaaatgcgta ccgatcataa agagctttct
    91081 gaacatctga tgttggtgga tctcgcccgt aatgacctgg cacgcatttg cacccccggc
    91141 agccgctacg tcgccgacct caccaaagtt gaccgttact cttacgtgat gcacctggtc
    91201 tcccgcgtgg tcggtgagct gcgccacgat ctcgacgccc tgcacgctta ccgcgcctgt
    91261 atgaatatgg ggacgttaag cggtgcgccg aaagtacgcg ctatgcagtt aattgccgag
    91321 gctgaaggtc gtcgccgcgg cagctacggc ggcgcggtag gttatttcac cgcgcacggc
    91381 gatctcgaca cctgcattgt gatccgctcg gcgctggtgg aaaacggtat cgctaccgtg
    91441 caagccggtg ctggcgtagt ccttgattct gttccgcagt cggaagccga cgaaacccgt
    91501 aataaagccc gcgctgtact gcgcgctatt gccaccgcgc atcatgcaca ggagacgttc
    91561 taatggctga cattctgctg ctcgataata tcgactcttt tacttacaac ctggcagatc
    91621 agttgcgcag caatggtcat aacgtggtga tttaccgcaa ccatattccg gcgcagacct
    91681 taattgaacg ccttgcgacg atgagcaatc cggtgctgat gctttctcct ggacccggtg
    91741 tgccgagcga agctggttgt atgcctgaac tgcttacccg cctgcgcggt aagctgccta
    91801 ttattggcat ttgccttggt catcaggcga ttgtcgaagc ttacgggggc tatgtcggtc
    91861 aggcgggcga aattcttcac ggtaaagcgt cgagcattga acatgacggt caggcgatgt
    91921 ttgccggatt aacaaacccg ctgccggtgg cgcgttatca ctcgctggtt ggcagtaata
    91981 ttccggccgg tttaaccatc aacgcccatt ttaatggcat ggtgatggcg gtgcgtcacg
    92041 atgcagatcg cgtttgcgga ttccagttcc acccggaatc cattctcacc acccagggcg
    92101 ctcgcctgct ggaacaaacg ctggcctggg cgcagcagaa actagagcca accaacacgc
    92161 tgcaaccgat tctggaaaaa ctgtatcagg cgcagaccct tagccagcag gaaagtcatc
    92221 agctattttc agcggtggta cgtggtgaac tgaaaccgga acacctggcg gcggcgctgg
    92281 tgagcatgaa aattcgcggc gagcacccga acgagatcgc cggagcagca accgcgctac
    92341 tggaaaacgc cgcgccgttc ccgcgtccgg attatctgtt tgccgatatc gtcggcaccg
    92401 gcggtgacgg cagcaacagc atcaatattt ccaccgccag tgcgtttgtc gccgcggcct
    92461 gtgggctgaa agtggcgaaa cacggcaacc gtagcgtctc cagtaaatct ggctcgtcgg
    92521 atctgctggc ggcgttcggt attaatcttg atatgaacgc cgataaatcg cgccaggcgc
    92581 tggatgagtt aggtgtatgt ttcctctttg caccgaaata tcacaccgga tttcgccatg
    92641 caatgccggt tcgccagcaa ctaaaaaccc gcaccctgtt caatgtgctg gggccattga
    92701 ttaacccggc gcatccgccg ctggcgttaa ttggtgttta tagtccggaa ctggtgctgc
    92761 cgattgccga aaccttacgc gtgctggggt atcaacgcgc ggcggtggtg cacagcggcg
    92821 ggatggatga agtttcatta cacgcgccga caatcgttgc cgagctgcat gacggcgaaa
    92881 ttaagagcta tcaattgacc gctgaagatt ttggcctgac tccctaccac caggagcaac
    92941 tggcaggcgg aacaccggaa gaaaaccgtg acattttaac acgcttgtta caaggtaaag
    93001 gcgacgccgc ccatgaagca gccgtcgcgg cgaatgtcgc catgttaatg cgcctgcatg
    93061 gccatgaaga tctgcaagcc aatgcgcaaa ccgttcttga ggtactgcgc agtggttccg
    93121 cttacgacag agtcaccgca ctggcggcac gagggtaaat gatgcaaacc gttttagcga
    93181 aaatcgtcgc agacaaggcg atttgggtag aagcccgcaa acagcagcaa ccgctggcca
    93241 gttttcagaa tgaggttcag ccgagcacgc gacattttta tgatgcgcta cagggtgcgc
    93301 gcacggcgtt tattctggag tgcaagaaag cgtcgccgtc aaaaggcgtg atctgtgatg
    93361 atttcgatcc agcacgcatt gccgccattt ataaacatta cgcttcggca atttcggtgc
    93421 tgactgatga gaaatatttt caggggagct ttgatttcct ccccatcgtc agccaaatcg
    93481 ccccgcagcc gattttatgt aaagacttca ttatcgaccc ttaccagatc tatctggcgc
    93541 gctattacca ggccgatgcc tgcttattaa tgctttcagt actggatgac gaacaatatc
    93601 gccagcttgc cgccgtcgcc cacagtctga agatgggtgt gctgaccgaa gtcagtaatg
    93661 aagaggaact ggagcgcgcc attgcattgg gggcaaaggt cgttggcatc aacaaccgcg
    93721 atctgcgtga tttgtcgatt gatctcaacc gtacccgcga gattgcgccg aaactggggc
    93781 acaacgtgac ggtaatcagc gaatcaggca tcaatactta cgctcaggtg cgcgagttaa
    93841 gccacttcgc tgacggtttt ctaattggtt cggcgttgat ggcccatgac gatttgcacg
    93901 ccgccgtgcg tcgggtgttg ctgggtgaga ataaagtatg tggcctgacg cgtgggcaag
    93961 atgctaaagc agcgtacgac gcgggcgcga tttacggtgg gttgattttt gttgcgacat
    94021 caccgcgttg cgtcaacgtt gaacaggcgc aggaagtgat ggctgcggca ccgttgcagt
    94081 atgttggcgt gttccgcaat cacgatattg ccgatgtgct ggacaaagct aaggtgttat
    94141 cactggtggc agtgcaactg catggtaatg aagatcagct gtatatcgat acgctgcgtg
    94201 aagctctgcc agcacacgtt gccatctgga aagcattaag cgtcggtgaa accctgcccg
    94261 cccgcgagct tcagcacgtt gataaatatg ttttagacaa cggccagggt ggaagcgggc
    94321 aacgtttcga ctggtcacta ttaaatggtc aatcgcttgg caacgttctg ctggcggggg
    94381 gcttaggcgc agataactgc gtggaagcgg cacaaaccgg ctgcgccgga cttgatttta
    94441 attctgctgt agagtcgcaa ccgggcatca aagacgcacg tcttttggcc tcggttttcc
    94501 agacgctgcg cgcatattaa ggaaaggaac aatgacaaca ttacttaacc cctattttgg
    94561 tgagtttggc ggcatgtacg tgccacaaat cctgatgcct gctctgcgcc agctggaaga
    94621 agcttttgtc agcgcgcaaa aagatcctga atttcaggct cagttcaacg acctgctgaa
    94681 aaactatgcc gggcgtccaa ccgcgctgac caaatgccag aacattacgg ctggcacgaa
    94741 caccacgctg tatctgaagc gcgaagattt gctgcacggc ggcgcgcata aaactaacca
    94801 ggtgctcggt caggctttac tggcgaagcg gatgggtaaa accgaaatca tcgccgaaac
    94861 cggggccggt cagcatggcg tggcgtcggc ccttgccagc gccctgctcg gcctgaaatg
    94921 ccgtatttat atgggggcca aagacgttga acgccagtcg cctaacgttt ttcgtatgcg
    94981 tttaatgggt gcggaagtga tcccggtgca tagcggttcc gcgacgctga aagatgcctg
    95041 taacgaggcg ctgcgcgact ggtccggcag ttatgaaacc gcgcactata tgctgggcac
    95101 cgcagctggc ccacatcctt atccgaccat tgtccgtgag tttcagcgga tgattggcga
    95161 agaaaccaaa gcacagattc tggaaagaga aggtcgcctg ccggatgccg ttatcgcctg
    95221 tgttggcggc ggttcgaatg ccatcggcat gtttgcagat ttcatcaacg aaaccaacgt
    95281 cggcctgatt ggtgtggagc ctggtggtca cggtatcgaa actggcgagc acggcgcacc
    95341 gctaaaacat ggtcgcgtgg gtatctattt cggtatgaaa gcgccgatga tgcaaaccga
    95401 agacgggcag attgaagaat cttactccat ctccgccgga ctcgattttc cgtccgtcgg
    95461 cccacaacac gcgtatctta acagcactgg acgcgctgat tatgtgtcaa ttaccgatga
    95521 tgaagcactg gaagccttca aaacgctgtg cctgcacgaa gggattatcc cggcgctgga
    95581 atcctcccac gccctggccc atgcgctgaa aatgatgcgc gaaaacccgg aaaaagagca
    95641 gctactggtg gttaaccttt ccggtcgcgg cgataaagac atcttcaccg ttcacgatat
    95701 tttgaaagca cgaggggaaa tctgatggaa cgctacgaat ctctgtttgc ccagttgaag
    95761 gagcgcaaag aaggcgcatt cgttcctttc gtcacgctcg gtgatccggg cattgagcag
    95821 tcgctgaaaa ttatcgatac gctaattgaa gccggtgctg acacgctgga gttaggcatc
    95881 cccttctccg acccactggc agatggcccg acgattcaaa acgccacact gcgtgccttt
    95941 gcggcaggtg tgaccccggc gcagtgcttt gagatgctgg cactcattcg ccagaaacac
    96001 ccgaccattc ccattggcct gctgatgtat gccaacctgg tgtttaacaa aggcattgat
    96061 gagttttatg cccagtgcga gaaagtcggc gtcgattcgg tgctggttgc cgatgtgcca
    96121 gttgaagagt ccgcgccctt ccgccaggcc gcgttgcgtc ataatgtcgc acctatcttc
    96181 atctgcccgc caaatgccga tgacgacctg ctgcgccaga tagcctctta cggtcgtggt
    96241 tacacatatt tgctgtcacg agcgggcgtg accggcgcag aaaaccgcgc cgcgttaccc
    96301 ctcaatcatc tggttgcgaa gctgaaagag tacaacgcgg cacctccatt gcagggattt
    96361 ggtatttccg ccccggatca ggtaaaagca gcgattgatg caggagctgc gggcgcgatt
    96421 tctggttctg cgattgttaa aatcatcgag caacatatta atgagccaaa gaaaatgctg
    96481 gcggcactga aagcttttgt acaaccgatg aaagcggcga cgcgcagtta atcccacagc
    96541 cgccagttcc gctggcggca ttttaacttt ctttaacgaa gccggaaaaa tcctaaattc
    96601 atttaatatt tatcttttta ccgtttcgct tacccctgtc gaacgtcaac ttacgtcatt
    96661 tttccgccca acagtaatat aatcaaacaa tttaatcccg caacataaca ccagtaaaat
    96721 caataatttt ctctaagtca cttattccgc aggcaattgt taatatatca agaacgttcg
    96781 tcataatata ttttcctact ttcttctcgt tacgcttaat ttgactaatc ctcattagcg
    96841 actaatttta atgagtatcg acacacaaca ctcatattaa tgaaacaatg caacgcaacg
    96901 ggagaaataa catggccgaa catcgtggtg gttcaggaaa tttcgccgaa gaccgtgaga
    96961 aggcatccga cgcaggccgt aaaggcggtc agcatagcgg cggtaatttt aaaaatgatc
    97021 cgcaacgcgc atctgaagcg ggtaaaaaag gcggtcaaca aagcggtggt aataaatcag
    97081 gaaaatcctg attcaccgtt aattattaat atcagctgat aaagataatt gcatctgcgg
    97141 gccgtatggc tcgcagtgat ttcactggag aaaaatatga atatgaagac cattgaagat
    97201 gtatttattc acctgctttc agatacctac agcgcagaaa aacaattaac ccgggcactg
    97261 gcaaaactcg caagagcaac atcaaacgaa aaattaagtc aggcttttca tgcgcacctc
    97321 gaggaaacac atggacagat tgaacgtatt gatcaagttg tggaatcaga atcgaatctg
    97381 aaaattaagc gcatgaaatg tgtggcaatg gaaggtctta ttgaagaagc taatgaggtc
    97441 atcgaaagta ccgagaaaaa cgaagtgcgt gatgccgcac tgattgccgc agcacagaaa
    97501 gtcgagcatt atgagattgc cagttacggg acattagcga cgctggctga acaattaggt
    97561 taccgtaaag cagcgaagct tctgaaagag accctggaag aagaaaaggc caccgacatc
    97621 aaactgactg atctggccct taataacgta aataagaaag ccgaaaataa agcctgaaat
    97681 atgaacttta acttttggtc attttataaa gaggacattt tcatgaatcg tattgaacat
    97741 tatcatgact ggttacgtga cgcccatgca atggaaaagc aagccgaatc tatgcttgaa
    97801 tccatggcca gccgtataga taattatcct gaactacgtg ctcgtattga acaacatctt
    97861 agtgaaacca aaaaccagat tgttcaactg gaaactattc ttgatcgtaa tgacatttca
    97921 cgttcagtca ttaaagattc catgagtaaa atggctgcgc ttgggcagtc aatcggtggt
    97981 atattccctt ctgatgaaat agtcaaaggc tctattagcg gatatgtctt cgagcaattt
    98041 gaaatcgcct gttacacctc tctattagca gcagcaaaaa atgccggtga tacagcctca
    98101 attccgatca tcgaagcgat tttaaatgag gaaaagcaaa tggccgactg gctgattcag
    98161 catattccgc aaacaactga gaaattttta attcgctctg aaactgatgg cgtagaagcg
    98221 aagaaataat accctttatg ccatgtcctt attgaccccg tatattacgg ggtcgttttt
    98281 gtgcggaatt aaaaacgata tcctgctgag aacataaaca cccacggatc gaggcgtacg
    98341 ctatcatgtt gctgtgcacc gcccagttta taattggcgg tggtatcgat
//
LOCUS       DAZFBR010000003        96214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFBR010000003 DAZFBR010000000
VERSION     DAZFBR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.003"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..283)
                     /gene="stfP"
                     /locus_tag="ACHJZ2_000202"
     CDS             complement(<1..283)
                     /gene="stfP"
                     /locus_tag="ACHJZ2_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein StfP"
                     /protein_id="HFO4566398.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVQTAL"
     gene            complement(287..871)
                     /gene="ymfQ"
                     /locus_tag="ACHJZ2_000203"
     CDS             complement(287..871)
                     /gene="ymfQ"
                     /locus_tag="ACHJZ2_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFO4566399.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPATTNSTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSQTYVIFKYPE"
     gene            complement(862..1920)
                     /locus_tag="ACHJZ2_000204"
     CDS             complement(862..1920)
                     /locus_tag="ACHJZ2_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO4566400.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIEPLAPVAGSDLYVFRPVAHKVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPVD
                     NISIAKNELAVLGTISWT"
     gene            complement(1907..2332)
                     /locus_tag="ACHJZ2_000205"
     CDS             complement(1907..2332)
                     /locus_tag="ACHJZ2_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO4566401.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(2332..2880)
                     /locus_tag="ACHJZ2_000206"
     CDS             complement(2332..2880)
                     /locus_tag="ACHJZ2_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4566402.1"
                     /translation="MRTIEAMQRQLLGLIARAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNSPKARFEMDLEVTGQVKDLCDSSGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(2880..3959)
                     /locus_tag="ACHJZ2_000207"
     CDS             complement(2880..3959)
                     /locus_tag="ACHJZ2_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO4566403.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEVTTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(3956..5284)
                     /locus_tag="ACHJZ2_000208"
     CDS             complement(3956..5284)
                     /locus_tag="ACHJZ2_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO4566404.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(5345..7180)
                     /locus_tag="ACHJZ2_000209"
     CDS             complement(5345..7180)
                     /locus_tag="ACHJZ2_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4566405.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYQAVDPAQSNNASGATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            complement(7322..7591)
                     /locus_tag="ACHJZ2_000210"
     CDS             complement(7322..7591)
                     /locus_tag="ACHJZ2_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO4566406.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(7591..7947)
                     /locus_tag="ACHJZ2_000211"
     CDS             complement(7591..7947)
                     /locus_tag="ACHJZ2_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4566407.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNKTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(7947..9443)
                     /locus_tag="ACHJZ2_000212"
     CDS             complement(7947..9443)
                     /locus_tag="ACHJZ2_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4566408.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMSSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPESTGAAATV
                     TLTVTGAATETGTVNVYVGRTRVQAPVTNGDNVTMIASSIQDAINAVPTLPFTASSLA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(9427..9597)
                     /locus_tag="ACHJZ2_000213"
     CDS             complement(9427..9597)
                     /locus_tag="ACHJZ2_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO4566409.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(9606..10166)
                     /locus_tag="ACHJZ2_000214"
     CDS             complement(9606..10166)
                     /locus_tag="ACHJZ2_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566410.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDELGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            complement(10163..10669)
                     /locus_tag="ACHJZ2_000215"
     CDS             complement(10163..10669)
                     /locus_tag="ACHJZ2_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566411.1"
                     /translation="MATPFFHVDVQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKKRAGFMTRIAPNQRNGKGNRMISGDFYP
                     AFLFFGVRGGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRRR"
     gene            complement(10644..10880)
                     /locus_tag="ACHJZ2_000216"
                     /pseudo
     CDS             complement(10644..10880)
                     /locus_tag="ACHJZ2_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
     gene            complement(11010..11747)
                     /locus_tag="ACHJZ2_000217"
     CDS             complement(11010..11747)
                     /locus_tag="ACHJZ2_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO4566412.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTRNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESTFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            complement(11701..11901)
                     /locus_tag="ACHJZ2_000218"
     CDS             complement(11701..11901)
                     /locus_tag="ACHJZ2_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4566413.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPYHQHA"
     gene            12016..12481
                     /locus_tag="ACHJZ2_000219"
                     /pseudo
     CDS             12016..12481
                     /locus_tag="ACHJZ2_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12520..12765)
                     /locus_tag="ACHJZ2_000220"
     CDS             complement(12520..12765)
                     /locus_tag="ACHJZ2_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4566414.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENKPQPVEQPEPVQPGTDGAMM"
     gene            complement(12801..12983)
                     /locus_tag="ACHJZ2_000221"
     CDS             complement(12801..12983)
                     /locus_tag="ACHJZ2_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4566415.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            complement(13130..15169)
                     /locus_tag="ACHJZ2_000222"
     CDS             complement(13130..15169)
                     /locus_tag="ACHJZ2_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4566416.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTVGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENGVNVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSSWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGSELLKNGGLISCRFKVPGDLVANQYVMALYWPVSSLPQGVTLTGDA
                     GNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     IPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            complement(15269..15829)
                     /locus_tag="ACHJZ2_000223"
     CDS             complement(15269..15829)
                     /locus_tag="ACHJZ2_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4566417.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            complement(15843..16028)
                     /locus_tag="ACHJZ2_000224"
     CDS             complement(15843..16028)
                     /locus_tag="ACHJZ2_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566418.1"
                     /translation="MFISCVPSKATPLILQAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISALKGFSTSF"
     gene            16385..16906
                     /locus_tag="ACHJZ2_000225"
     CDS             16385..16906
                     /locus_tag="ACHJZ2_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4566419.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            complement(16941..17852)
                     /locus_tag="ACHJZ2_000226"
     CDS             complement(16941..17852)
                     /locus_tag="ACHJZ2_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4566420.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENAETARNNLDVYSRSEGDNRYLRR
                     DQNGADIQDKGTFIDNLGLRETVNKAADALPSAGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGIDVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(17852..18412)
                     /locus_tag="ACHJZ2_000227"
     CDS             complement(17852..18412)
                     /locus_tag="ACHJZ2_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO4566421.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEENI"
     gene            complement(18403..19485)
                     /locus_tag="ACHJZ2_000228"
     CDS             complement(18403..19485)
                     /locus_tag="ACHJZ2_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO4566422.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            complement(19485..19922)
                     /locus_tag="ACHJZ2_000229"
     CDS             complement(19485..19922)
                     /locus_tag="ACHJZ2_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO4566423.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GVATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            complement(19915..20529)
                     /locus_tag="ACHJZ2_000230"
     CDS             complement(19915..20529)
                     /locus_tag="ACHJZ2_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4566424.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNITVDKNLHVKGNV
                     SIDGTGRSQGAFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            complement(20519..21643)
                     /locus_tag="ACHJZ2_000231"
     CDS             complement(20519..21643)
                     /locus_tag="ACHJZ2_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO4566425.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            complement(21627..22976)
                     /locus_tag="ACHJZ2_000232"
     CDS             complement(21627..22976)
                     /locus_tag="ACHJZ2_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFO4566426.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            complement(22963..25038)
                     /locus_tag="ACHJZ2_000233"
     CDS             complement(22963..25038)
                     /locus_tag="ACHJZ2_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4566427.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSQAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGMGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPSSGGAGFWGRMMGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGAL
                     WAGAMAAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNY
                     LGGWLTEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNV
                     WNGGNYGLW"
     gene            complement(25165..25641)
                     /locus_tag="ACHJZ2_000234"
     CDS             complement(25165..25641)
                     /locus_tag="ACHJZ2_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO4566428.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            complement(25656..26021)
                     /locus_tag="ACHJZ2_000235"
     CDS             complement(25656..26021)
                     /locus_tag="ACHJZ2_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4566429.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            complement(26030..27529)
                     /locus_tag="ACHJZ2_000236"
     CDS             complement(26030..27529)
                     /locus_tag="ACHJZ2_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4566430.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATEFWGRGSMIALMVAEFIAINPDAELYAIAQGSGT
                     GQAQACLMNIIGTASQDGVLHIYIGGRRYLLAVTKGQKGKELIDALVSVINADADAPF
                     SAYAGQVADNASGLKGSMGINARFTGECSVHDIRVNYHDGETTPGGLTIELGYPKTKA
                     TNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWMAHTGT
                     FGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALPS
                     RMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLDV
                     ETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWVE
                     LGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            complement(27526..27771)
                     /locus_tag="ACHJZ2_000237"
     CDS             complement(27526..27771)
                     /locus_tag="ACHJZ2_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO4566431.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRLLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKSAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            complement(27772..28332)
                     /locus_tag="ACHJZ2_000238"
     CDS             complement(27772..28332)
                     /locus_tag="ACHJZ2_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO4566432.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            complement(28329..28748)
                     /locus_tag="ACHJZ2_000239"
     CDS             complement(28329..28748)
                     /locus_tag="ACHJZ2_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO4566433.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            complement(28745..29098)
                     /locus_tag="ACHJZ2_000240"
     CDS             complement(28745..29098)
                     /locus_tag="ACHJZ2_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO4566434.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVSPEQLARLEAEPCLTVRILQTPADDAGSVAGVVHAVAGTDLAEAGQDTPR
                     KKTGNKAKQAKKARA"
     gene            complement(29142..30089)
                     /locus_tag="ACHJZ2_000241"
     CDS             complement(29142..30089)
                     /locus_tag="ACHJZ2_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO4566435.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            complement(30089..31213)
                     /locus_tag="ACHJZ2_000242"
     CDS             complement(30089..31213)
                     /locus_tag="ACHJZ2_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO4566436.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTSLQTD
                     TVTVPDKKTATAALSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            complement(31390..31863)
                     /locus_tag="ACHJZ2_000243"
     CDS             complement(31390..31863)
                     /locus_tag="ACHJZ2_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO4566437.1"
                     /translation="MSSIDAAVVVDVARLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            31902..31982
                     /locus_tag="ACHJZ2_000244"
     tRNA            31902..31982
                     /locus_tag="ACHJZ2_000244"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(31982..33307)
                     /locus_tag="ACHJZ2_000245"
     CDS             complement(31982..33307)
                     /locus_tag="ACHJZ2_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFO4566438.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGQVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSVKHQNDGIALTPEDFAQLPAMLAAPDAVLWDHVHQNLLYITETRDG
                     TAKIAVNAPYGVKRQPDKLDVVINAYRVNKFDIEKAIEGGKLELLEGKL"
     gene            complement(33291..34880)
                     /locus_tag="ACHJZ2_000246"
     CDS             complement(33291..34880)
                     /locus_tag="ACHJZ2_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO4566439.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            complement(34880..36544)
                     /locus_tag="ACHJZ2_000247"
     CDS             complement(34880..36544)
                     /locus_tag="ACHJZ2_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566440.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMLRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAIEGGYRDDDEAYHEFDRGCW"
     gene            complement(36544..37125)
                     /locus_tag="ACHJZ2_000248"
     CDS             complement(36544..37125)
                     /locus_tag="ACHJZ2_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO4566441.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            complement(37128..37418)
                     /locus_tag="ACHJZ2_000249"
     CDS             complement(37128..37418)
                     /locus_tag="ACHJZ2_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO4566442.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            complement(37415..37723)
                     /locus_tag="ACHJZ2_000250"
     CDS             complement(37415..37723)
                     /locus_tag="ACHJZ2_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO4566443.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            complement(37704..37931)
                     /locus_tag="ACHJZ2_000251"
     CDS             complement(37704..37931)
                     /locus_tag="ACHJZ2_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4566444.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            complement(37942..38160)
                     /locus_tag="ACHJZ2_000252"
     CDS             complement(37942..38160)
                     /locus_tag="ACHJZ2_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566445.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            complement(38144..38572)
                     /locus_tag="ACHJZ2_000253"
     CDS             complement(38144..38572)
                     /locus_tag="ACHJZ2_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566446.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            complement(38607..39107)
                     /locus_tag="ACHJZ2_000254"
     CDS             complement(38607..39107)
                     /locus_tag="ACHJZ2_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4566447.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            complement(39179..39604)
                     /locus_tag="ACHJZ2_000255"
     CDS             complement(39179..39604)
                     /locus_tag="ACHJZ2_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO4566448.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            complement(39674..40183)
                     /locus_tag="ACHJZ2_000256"
     CDS             complement(39674..40183)
                     /locus_tag="ACHJZ2_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO4566449.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSVLLAVTGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMHRQGFITDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            complement(40180..40476)
                     /locus_tag="ACHJZ2_000257"
     CDS             complement(40180..40476)
                     /locus_tag="ACHJZ2_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001509933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566450.1"
                     /translation="MDSSSQLFWYLYITSFFGAFVITRWLCRKVISIFHKKYLIGQAA
                     TSLINALARYHKGECYSRIKTDDGFNIFVIPPEYRVQLDRENNTYHIRRSVDTA"
     gene            complement(40466..40663)
                     /locus_tag="ACHJZ2_000258"
                     /pseudo
     CDS             complement(40466..40663)
                     /locus_tag="ACHJZ2_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40656..40988)
                     /locus_tag="ACHJZ2_000259"
     CDS             complement(40656..40988)
                     /locus_tag="ACHJZ2_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566451.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDMPEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            complement(41004..41354)
                     /locus_tag="ACHJZ2_000260"
     CDS             complement(41004..41354)
                     /locus_tag="ACHJZ2_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566452.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYEPCGDDTW
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            complement(41369..41680)
                     /locus_tag="ACHJZ2_000261"
     CDS             complement(41369..41680)
                     /locus_tag="ACHJZ2_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566453.1"
                     /translation="MITTLFVESDEPLVCAVGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISALP
                     E"
     gene            complement(41677..42228)
                     /locus_tag="ACHJZ2_000262"
     CDS             complement(41677..42228)
                     /locus_tag="ACHJZ2_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO4566454.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRI
                     KRSFWGVHNRIVLLRGTYPELLKCKRLRFKPDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVRLIRELRDAESPRRLTFREIGEKFELSE
                     GTVSFIYHRRRTAEDVVLRELMP"
     gene            complement(42232..42747)
                     /locus_tag="ACHJZ2_000263"
     CDS             complement(42232..42747)
                     /locus_tag="ACHJZ2_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566455.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVSCRSVFE
                     FSISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVEWFCVDDVIYLECGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            complement(42747..43280)
                     /locus_tag="ACHJZ2_000264"
     CDS             complement(42747..43280)
                     /locus_tag="ACHJZ2_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566456.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEYVLRY
                     LIVDRTAAPATISAEVA"
     gene            complement(43284..43826)
                     /locus_tag="ACHJZ2_000265"
     CDS             complement(43284..43826)
                     /locus_tag="ACHJZ2_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566457.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDRISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDELCPEGPAIAAMVEA
                     AERDLRKHPDFLVWLCEKLGVMRIPSMFNTDSWWTPSFSRDALCVVFKVGAYGREING
                     CIPEECQEIKHSEYVALTEE"
     gene            complement(43924..44454)
                     /locus_tag="ACHJZ2_000266"
     CDS             complement(43924..44454)
                     /locus_tag="ACHJZ2_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4566458.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            complement(44466..44759)
                     /locus_tag="ACHJZ2_000267"
     CDS             complement(44466..44759)
                     /locus_tag="ACHJZ2_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566459.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            complement(44764..45036)
                     /locus_tag="ACHJZ2_000268"
     CDS             complement(44764..45036)
                     /locus_tag="ACHJZ2_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566460.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            complement(45033..45314)
                     /locus_tag="ACHJZ2_000269"
     CDS             complement(45033..45314)
                     /locus_tag="ACHJZ2_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO4566461.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            complement(45316..45570)
                     /locus_tag="ACHJZ2_000270"
     CDS             complement(45316..45570)
                     /locus_tag="ACHJZ2_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566462.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            complement(45583..45804)
                     /locus_tag="ACHJZ2_000271"
     CDS             complement(45583..45804)
                     /locus_tag="ACHJZ2_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566463.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            complement(45807..46739)
                     /locus_tag="ACHJZ2_000272"
     CDS             complement(45807..46739)
                     /locus_tag="ACHJZ2_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4566464.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRTFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            complement(46811..48901)
                     /locus_tag="ACHJZ2_000273"
     CDS             complement(46811..48901)
                     /locus_tag="ACHJZ2_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO4566465.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVAVQQPAVDDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            complement(48912..49178)
                     /locus_tag="ACHJZ2_000274"
     CDS             complement(48912..49178)
                     /locus_tag="ACHJZ2_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4566466.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            49335..49946
                     /locus_tag="ACHJZ2_000275"
     CDS             49335..49946
                     /locus_tag="ACHJZ2_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4566467.1"
                     /translation="MDKEEKTVSSAERFIDMEGINRFSERLAMAMGNMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLEWLATGKNEDECGKIENNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
     gene            complement(50314..50490)
                     /locus_tag="ACHJZ2_000276"
                     /pseudo
     CDS             complement(50314..50490)
                     /locus_tag="ACHJZ2_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
     gene            complement(50487..50810)
                     /locus_tag="ACHJZ2_000277"
     CDS             complement(50487..50810)
                     /locus_tag="ACHJZ2_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4566468.1"
                     /translation="MLLKMEEIKLQLRLDDDFSDEDELLELLGKAAQSRTENFLNRKL
                     YATADDRPADDPDGLVISDDVKLALLLLVSHFYENRSTVTDVEKMELPMSFNWLVAPY
                     RLIPL"
     gene            complement(50889..52118)
                     /locus_tag="ACHJZ2_000278"
     CDS             complement(50889..52118)
                     /locus_tag="ACHJZ2_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO4566469.1"
                     /translation="MKLHELKQKRNTIATDMRALNEKIGDNAWTEEQRTEWNKAKSEL
                     EALDERIAREEELRRQDQAYIESNEEEQRQNLDPENNPQQDEKRAQVFDKWMRHGASE
                     LTSEERKALRELRAQGVAQDEKGGYTVPETFLAKVVEKMKSYGGIASVAQILTTSDGR
                     TMEWATADGTSEVGVLLGENEEAGEEDTDFGMGSLGALKMTSKIIRVSNELLQDSAID
                     MEAYLARRIAERIGRGEARYLIQGTGAGTPKQPKGLVASVTGTTQTAAANTVKWQEIL
                     ALKHSIDPAYRRGPKFRLAFNDNTLKLISEMEDGQGRPLWLPDIVGVAPASVLNVPYV
                     IDQEIDDIGAGKKFMFCGDFDRFIIRRVRYMILKRLVERYAEYDQTGFLAFHRFDCIL
                     EDTSAIKALVGKGSVGG"
     gene            complement(52129..52731)
                     /locus_tag="ACHJZ2_000279"
     CDS             complement(52129..52731)
                     /locus_tag="ACHJZ2_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO4566470.1"
                     /translation="MNDREIRCYSGEVRAERHDDNPAHIIGYGSVFDCRSELIFGSFR
                     EIIRPGAFDDVLGDDVRALFNHDPNFILGRSAAGTLNLSVDERGLRYDIQAPETQTIR
                     DLVLAPMQRGDINQSSFAFRVARDGEEWYQDEDGVVIREITRFSRLLDVSPVTYPAYQ
                     EADSAVRSMKAWQEARNSGALQKAINQRMARERVLTLLNA"
     gene            complement(52724..53950)
                     /locus_tag="ACHJZ2_000280"
     CDS             complement(52724..53950)
                     /locus_tag="ACHJZ2_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4566471.1"
                     /translation="MLLDALFRSKSLENPSTPITGDAVDTDGLFRTDVYVSPETAMKL
                     AAVYSCIYVLSSSLAQMPLHVMRRHNGKVEPARDHPAFYLVHDEPNTWQTSYKWRELK
                     QRHILGWGNGYTWVKRNRRGEVISLDCCMPWETTLMNTGGRYTYGLYNEYGAFAISPD
                     DMIHIRALGNNQKMGLSPIMQHAETIGMGMSGQKYTESFFSGNARPAGIVSVKSGLNK
                     DSWGWLKDQWQKASQALRSQENKTMLLPADLDYKALTVSPVDAQIIDMMKLNRSMIAG
                     IFNIPAHMINDLEKATFSNISAQAIQFVRYTMMPWVTNWEQELNRRLFTRAELAAGYY
                     VRFNLTGLLRGTPQERAQFYHFAITDGWMSRNEARAFEDMNPVEGLDEMLVSVNAANP
                     AGDFKPPKNDEGKTNE"
     gene            complement(53940..54101)
                     /locus_tag="ACHJZ2_000281"
     CDS             complement(53940..54101)
                     /locus_tag="ACHJZ2_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566472.1"
                     /translation="MKSLIADVIGLAGFGLLTCGFYLQFGMAPALMLSGGLLLVGALA
                     MARRGTRAA"
     gene            complement(54098..55831)
                     /locus_tag="ACHJZ2_000282"
     CDS             complement(54098..55831)
                     /locus_tag="ACHJZ2_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4566473.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     SRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWIDPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTAGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPITIIQNYTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKTIPGNDDVVKPVKEQAENKIDGAVALIMAIGRAMLKEPDDFLS
                     SLDPDDALLIL"
     gene            complement(55828..56322)
                     /locus_tag="ACHJZ2_000283"
     CDS             complement(55828..56322)
                     /locus_tag="ACHJZ2_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO4566474.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(56448..56798)
                     /locus_tag="ACHJZ2_000284"
     CDS             complement(56448..56798)
                     /locus_tag="ACHJZ2_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO4566475.1"
                     /translation="MPPRTPKACRVRGCRHTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRGRVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERLK"
     gene            57324..57617
                     /gene="iss"
                     /locus_tag="ACHJZ2_000285"
     CDS             57324..57617
                     /gene="iss"
                     /locus_tag="ACHJZ2_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO4566476.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(57708..57890)
                     /gene="rzoD"
                     /locus_tag="ACHJZ2_000286"
     CDS             complement(57708..57890)
                     /gene="rzoD"
                     /locus_tag="ACHJZ2_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO4566477.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(58107..58583)
                     /locus_tag="ACHJZ2_000287"
     CDS             complement(58107..58583)
                     /locus_tag="ACHJZ2_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO4566478.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(58587..58922)
                     /locus_tag="ACHJZ2_000288"
     CDS             complement(58587..58922)
                     /locus_tag="ACHJZ2_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO4566479.1"
                     /translation="MPHNPNTWPDWLELFQSWWRGDTPLGAVIMSIVMAGLRIAYFGG
                     GGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVI
                     GNKFGGSKE"
     gene            complement(59059..59361)
                     /locus_tag="ACHJZ2_000289"
                     /pseudo
     CDS             complement(59059..59361)
                     /locus_tag="ACHJZ2_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            complement(59631..59816)
                     /locus_tag="ACHJZ2_000290"
     CDS             complement(59631..59816)
                     /locus_tag="ACHJZ2_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566480.1"
                     /translation="MKHHEQIEIEAAKVVAELFAGNASPMETFGITWSQTQMLERKNP
                     GVVIKLVPDDGKKLAYC"
     gene            complement(60008..60550)
                     /locus_tag="ACHJZ2_000291"
     CDS             complement(60008..60550)
                     /locus_tag="ACHJZ2_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566481.1"
                     /translation="MMFQAFQTAVFKNVAEYLKGRFPHKSLGLVYADELVLKDMEFLK
                     ANNKLRWDPGLKSRVFMDMMEEHPIKLVVYYRGEPIGFAFGCYCKPKNAVHICWMEKR
                     NDAHEDLDHQMLGIVLDCFSAYARFLNLQGESIDSIALVSPVDGAIRYYTESGFEYIA
                     DYERGGCAMVLRSVLPNQSV"
     gene            complement(60762..61514)
                     /gene="ydfT"
                     /locus_tag="ACHJZ2_000292"
     CDS             complement(60762..61514)
                     /gene="ydfT"
                     /locus_tag="ACHJZ2_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4566482.1"
                     /translation="MNFEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHDVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSRLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTR"
     gene            complement(61528..62517)
                     /locus_tag="ACHJZ2_000293"
     CDS             complement(61528..62517)
                     /locus_tag="ACHJZ2_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4566483.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(62525..63334)
                     /locus_tag="ACHJZ2_000294"
     CDS             complement(62525..63334)
                     /locus_tag="ACHJZ2_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO4566484.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(63354..63743)
                     /locus_tag="ACHJZ2_000295"
     CDS             complement(63354..63743)
                     /locus_tag="ACHJZ2_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4566485.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(63740..64066)
                     /locus_tag="ACHJZ2_000296"
     CDS             complement(63740..64066)
                     /locus_tag="ACHJZ2_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO4566486.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHIAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            complement(64063..64716)
                     /locus_tag="ACHJZ2_000297"
     CDS             complement(64063..64716)
                     /locus_tag="ACHJZ2_000297"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO4566487.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRYELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(64716..65210)
                     /gene="yfdN"
                     /locus_tag="ACHJZ2_000298"
     CDS             complement(64716..65210)
                     /gene="yfdN"
                     /locus_tag="ACHJZ2_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4566488.1"
                     /translation="MSMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAVSGEEWYLSGN
                     YVGA"
     gene            complement(65207..66148)
                     /locus_tag="ACHJZ2_000299"
     CDS             complement(65207..66148)
                     /locus_tag="ACHJZ2_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4566489.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTDEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEHAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(66138..66317)
                     /locus_tag="ACHJZ2_000300"
     CDS             complement(66138..66317)
                     /locus_tag="ACHJZ2_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4566490.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(66493..67044)
                     /gene="ymfL"
                     /locus_tag="ACHJZ2_000301"
     CDS             complement(66493..67044)
                     /gene="ymfL"
                     /locus_tag="ACHJZ2_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO4566491.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(67037..67297)
                     /locus_tag="ACHJZ2_000302"
     CDS             complement(67037..67297)
                     /locus_tag="ACHJZ2_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4566492.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            67395..68087
                     /locus_tag="ACHJZ2_000303"
     CDS             67395..68087
                     /locus_tag="ACHJZ2_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4566493.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            complement(68578..68823)
                     /locus_tag="ACHJZ2_000304"
     CDS             complement(68578..68823)
                     /locus_tag="ACHJZ2_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566494.1"
                     /translation="MTFDEKELDNAINKIIVTSLFSCLSDTQQKQFYESAFNMIERCC
                     FCDADELPEKIRKQLADALRVRLSDQFSEMYSPNLDK"
     gene            68936..69255
                     /locus_tag="ACHJZ2_000305"
                     /pseudo
     CDS             68936..69255
                     /locus_tag="ACHJZ2_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            69337..69873
                     /gene="yfdR"
                     /locus_tag="ACHJZ2_000306"
     CDS             69337..69873
                     /gene="yfdR"
                     /locus_tag="ACHJZ2_000306"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4566495.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPQAPGHA
                     YGMFMERFNELSELRKCA"
     gene            69864..70226
                     /locus_tag="ACHJZ2_000307"
     CDS             69864..70226
                     /locus_tag="ACHJZ2_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566496.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            70226..70531
                     /gene="yfdT"
                     /locus_tag="ACHJZ2_000308"
     CDS             70226..70531
                     /gene="yfdT"
                     /locus_tag="ACHJZ2_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFO4566497.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            70447..70881
                     /locus_tag="ACHJZ2_000309"
     CDS             70447..70881
                     /locus_tag="ACHJZ2_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4566498.1"
                     /translation="MGYDSRLDRLAATSRYPFFNNVTTRGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            70803..71921
                     /locus_tag="ACHJZ2_000310"
     CDS             70803..71921
                     /locus_tag="ACHJZ2_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4566499.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRV
                     AWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKM
                     RYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRY
                     AHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(71936..72011)
                     /locus_tag="ACHJZ2_000311"
     tRNA            complement(71936..72011)
                     /locus_tag="ACHJZ2_000311"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71976..71978),aa:Thr,seq:cgt)
     gene            complement(72126..73379)
                     /gene="proA"
                     /locus_tag="ACHJZ2_000312"
     CDS             complement(72126..73379)
                     /gene="proA"
                     /locus_tag="ACHJZ2_000312"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO4566500.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(73391..74494)
                     /gene="proB"
                     /locus_tag="ACHJZ2_000313"
     CDS             complement(73391..74494)
                     /gene="proB"
                     /locus_tag="ACHJZ2_000313"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO4566501.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            74782..75837
                     /gene="phoE"
                     /locus_tag="ACHJZ2_000314"
     CDS             74782..75837
                     /gene="phoE"
                     /locus_tag="ACHJZ2_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO4566502.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(75876..76277)
                     /gene="crl"
                     /locus_tag="ACHJZ2_000315"
     CDS             complement(75876..76277)
                     /gene="crl"
                     /locus_tag="ACHJZ2_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO4566503.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(76335..77579)
                     /gene="frsA"
                     /locus_tag="ACHJZ2_000316"
     CDS             complement(76335..77579)
                     /gene="frsA"
                     /locus_tag="ACHJZ2_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO4566504.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(77671..78129)
                     /gene="gpt"
                     /locus_tag="ACHJZ2_000317"
     CDS             complement(77671..78129)
                     /gene="gpt"
                     /locus_tag="ACHJZ2_000317"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO4566505.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            78390..79847
                     /gene="pepD"
                     /locus_tag="ACHJZ2_000318"
     CDS             78390..79847
                     /gene="pepD"
                     /locus_tag="ACHJZ2_000318"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO4566506.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(79904..80425)
                     /gene="prfH"
                     /locus_tag="ACHJZ2_000319"
                     /pseudo
     CDS             complement(79904..80425)
                     /gene="prfH"
                     /locus_tag="ACHJZ2_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(80424..80627)
                     /locus_tag="ACHJZ2_000320"
                     /pseudo
     CDS             complement(80424..80627)
                     /locus_tag="ACHJZ2_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(80946..81398)
                     /gene="yafP"
                     /locus_tag="ACHJZ2_000321"
     CDS             complement(80946..81398)
                     /gene="yafP"
                     /locus_tag="ACHJZ2_000321"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4566507.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(81408..81806)
                     /gene="yafO"
                     /locus_tag="ACHJZ2_000322"
     CDS             complement(81408..81806)
                     /gene="yafO"
                     /locus_tag="ACHJZ2_000322"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO4566508.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(81809..82102)
                     /gene="yafN"
                     /locus_tag="ACHJZ2_000323"
     CDS             complement(81809..82102)
                     /gene="yafN"
                     /locus_tag="ACHJZ2_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO4566509.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(82154..83209)
                     /gene="dinB"
                     /locus_tag="ACHJZ2_000324"
     CDS             complement(82154..83209)
                     /gene="dinB"
                     /locus_tag="ACHJZ2_000324"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO4566510.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(83280..84065)
                     /gene="lafU"
                     /locus_tag="ACHJZ2_000325"
     CDS             complement(83280..84065)
                     /gene="lafU"
                     /locus_tag="ACHJZ2_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO4566511.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            84037..85749
                     /locus_tag="ACHJZ2_000326"
                     /pseudo
     CDS             84037..85749
                     /locus_tag="ACHJZ2_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(85894..86391)
                     /gene="rayT"
                     /locus_tag="ACHJZ2_000327"
                     /pseudo
     CDS             complement(85894..86391)
                     /gene="rayT"
                     /locus_tag="ACHJZ2_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(86567..87316)
                     /gene="yafL"
                     /locus_tag="ACHJZ2_000328"
     CDS             complement(86567..87316)
                     /gene="yafL"
                     /locus_tag="ACHJZ2_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4566512.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            87526..87786
                     /gene="dinJ"
                     /locus_tag="ACHJZ2_000329"
     CDS             87526..87786
                     /gene="dinJ"
                     /locus_tag="ACHJZ2_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO4566513.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGV"
     gene            87789..88065
                     /gene="yafQ"
                     /locus_tag="ACHJZ2_000330"
                     /pseudo
     CDS             87789..88065
                     /gene="yafQ"
                     /locus_tag="ACHJZ2_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
     gene            88221..88961
                     /gene="dpaA"
                     /locus_tag="ACHJZ2_000331"
     CDS             88221..88961
                     /gene="dpaA"
                     /locus_tag="ACHJZ2_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO4566514.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(88932..89699)
                     /gene="yafJ"
                     /locus_tag="ACHJZ2_000332"
     CDS             complement(88932..89699)
                     /gene="yafJ"
                     /locus_tag="ACHJZ2_000332"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO4566515.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(89804..90382)
                     /gene="lpcA"
                     /locus_tag="ACHJZ2_000333"
     CDS             complement(89804..90382)
                     /gene="lpcA"
                     /locus_tag="ACHJZ2_000333"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO4566516.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            90622..93066
                     /gene="fadE"
                     /locus_tag="ACHJZ2_000334"
     CDS             90622..93066
                     /gene="fadE"
                     /locus_tag="ACHJZ2_000334"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO4566517.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(93109..93555)
                     /gene="ivy"
                     /locus_tag="ACHJZ2_000335"
     CDS             complement(93109..93555)
                     /gene="ivy"
                     /locus_tag="ACHJZ2_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO4566518.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            93736..94506
                     /gene="yafV"
                     /locus_tag="ACHJZ2_000336"
     CDS             93736..94506
                     /gene="yafV"
                     /locus_tag="ACHJZ2_000336"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO4566519.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
     gene            complement(94624..95796)
                     /locus_tag="ACHJZ2_000337"
     CDS             complement(94624..95796)
                     /locus_tag="ACHJZ2_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HFO4566520.1"
                     /translation="MSIQSLLDYISVIPDIRQQGKVKHKLSDILFLTVCAVIAGADEW
                     QEIEDFGHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKIFIEWMQECHEITD
                     GEIIAIDGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLN
                     LLDLKKNLITIDAMGCQKDIASKIKDKKADYLLAVKGNQGKLHHAFEEKFPVNVFSNY
                     KGDSFSTQEISHGRKETRLHIVSNVTPEFCDFEFEWKGLKKLCVALSFRQKKEDKSAE
                     GVSIRYYISSKDMDAKEFAHAIRAHWLIEHSLHWVLDVKMNEDASRIRRGNAAEIISG
                     IKKMALNLLRDCKDIKGGVKRKRKKVALNTCYIEEVLASCSELGFRTDKMKNLTQI"
     gene            95877..96062
                     /locus_tag="ACHJZ2_000338"
     CDS             95877..96062
                     /locus_tag="ACHJZ2_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566521.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFSNCYHNNQHHNSLHN"
     gene            complement(95977..>96214)
                     /gene="ydcD"
                     /locus_tag="ACHJZ2_000339"
     CDS             complement(95977..>96214)
                     /gene="ydcD"
                     /locus_tag="ACHJZ2_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HFO4566522.1"
                     /translation="GVVDINLNFFNDILYSVRLKNISKLENMEFCATKQRVYFSDKNK
                     KASYKIINYGDYYDVDYYDNNLKNEVFDWIGKWS"
BASE COUNT        22705 a        26519 c        23492 g        23498 t
ORIGIN      
        1 cgagagccgt ttgcacagtg ccatccgatt tgatatcgcc aaacggattc ttgcggctta
       61 acagcagcgc gcgaagtgcg gtaagtaact ggtcgtgccg ccccttctcc aggctggcac
      121 cggatgcctc caccacgctg caaagttcct cctgcaacat gtcaaagtag tcatcatcca
      181 gatcggtggc aggcgtgccg gtctgggggt taccacgggt aaaaccgttc ttacccgcgc
      241 cgaacttatc cttctgcgcg gttttcgtgt ctatacgatg catggattac tccggatatt
      301 taaaaattac gtaggtttgc gacgggcaga gtttgttaag cacacactcg acaacggtgt
      361 caccccagat acgcagtgcg gaatcacagg gatcgccaca tgtcatccag gtggagttgg
      421 tggtggctgg catgttgacc tgccagtaat accgccattc cggcgcattc accgcgtcag
      481 tacaggccga tgagcaggtg aaagtgcttt tgtcgtatcg cgtgatggtg gcatctggtc
      541 tgcccagggc agcaagctgt gcaagataaa aatcctcgtt gatgccgccc gccaggttaa
      601 ccttcgcatc cagccgttgc tgacgctggc gaagggtctg cgttcccgcc ggaatacatt
      661 catccggcag accgcacaga cgctcccagc ggtttatcag ttcagtggtg gtgcgaggat
      721 ccagctcccg catcagggca tccgcacgct gatgaacacg ggttaatgac ggtgccgcac
      781 cggcaatcgc cggatcgctg gctgaccacg ccggaccggg cggcaacagt gccgataaca
      841 ggcggatgta atcatcgttt gtcacgtcca tgaaatcgtc cccagtaccg ccagttcgtt
      901 tttcgcaatg gagatattgt ccaccggtgc aagcaactga tggctgtatt ccccgttcgc
      961 accggaaatc gcctcactga tacgcgatac cttgagttct ccctgcggat aaccatcacg
     1021 cagcaggaac gaacgcaact ccgcggtaat ggcagcccgt atttccggtg tgtccggcgt
     1081 cacgcggata tgaaaatcca ctttatgcgc caccggcctg aatacataca aatcagagcc
     1141 tgccaccggg gccagtggct caatgtgttg tcttgccgcc gtttccgttg attcttccgg
     1201 aatgggatta atcaggtcac tgctggcaat catcacaccg acagttcccg ttcccatcca
     1261 gtgacggtat gtccatgcgc gggtaatgcc gggcacttct ttagcccaga caacatagtc
     1321 cccgtcagcc ccgccctgag gcgtccagta ataccgctca atgacgcggg cgcgccacgt
     1381 ttccagatct tcagtatcga atccgccagt cagggtatct gcaacaccgg aagacggcag
     1441 accattcacc ggcgtgacca ggattaatgc cgtaccgtcg tcagcgttac cgaccgcgcc
     1501 tgcacttgag caggcgatcg gcacgcgcag gacaccaccg gagctggttg catcagcagt
     1561 tgccgtgtac tgaaccaggt catcgcgctg aatcacgctc ccggcggtca ccttcaggcc
     1621 atcgctgaca ccttcccagc gcatataccc gctggcagcc gtggccccct tgcgcggaca
     1681 ccgtttcatc gcagcatgtc gcgccagcca ggactcatcg cacaggtcag gcagcatgtt
     1741 cattgccaga taatcgatgt aaccgtaaac cgtatgcagc gccgccgcat acacctttgc
     1801 ccgcacgtct tcatccatgc gccggagcgt gtcgctgacg tccagcctgg cgaataaatc
     1861 gttacggagc atactgatat tttctgccag cgtcgggcgc tgaaattcac tgtccgccat
     1921 gcgttatcgc actccacaga tcatcaaaag aaatcattac cggtccgtca cgacgccaga
     1981 gggtgatact gttacccagc tcattaatcc cggtgcggcg gatatccaga tcaatacggg
     2041 acaccacgcc gtcatcaatc atccattgca ggcattcgcg gatatacccc cttactgtct
     2101 gcaccagctg attggtcagt ttgctgcgct gaagcagcca cagtcgtgag ccgtaacggt
     2161 cattctgtac cgcaggccag gtatcccccc accatcccat cgggacgtcg gcgttgtcat
     2221 caggctccgc ccgccgccag gtaaacaggg aaatcaccac ggcgcgggtc agcggatcca
     2281 gcggtgcgct ggcgcaggtg cgtttaccgt tcaccgtcag ccacagttcc atcatgcctc
     2341 catcgcttta tccggtttgt cggtgttact gccctgaccg ttctctctgt gacgatgccc
     2401 gttataggca agccgcatcg ctgacatggt agtgccgctg gagtcgcaca ggtctttcac
     2461 ctgtcctgtc acttccaggt ccatttcaaa acgtgcttta ggtgaattgc gaaacgtgat
     2521 cgttttacct gcaccgtcca ccacgatccc ctcccgggtc agcgtcacag actgcccctg
     2581 atcgtcatag acagccacct cacccgtctg caaccctttc aggcggtagc gccggtccga
     2641 caccgtaaca accaccgcat gagaacggtc gccatccgga aacagcacca ccgcttccgc
     2701 gccgctgttt gcccttgcgg taaaaccgta gggttcaaga tgttcaaccc cggctttggg
     2761 ttcaccggca atcagggaca catccacggt ctgacatttc gtggcggcac tgatgctttt
     2821 caccactgcc cgcgcaatca ggccgagaag ttgtcgctgc atggcttcaa tcgtcctcat
     2881 cagaacgggt cctcctgtac tctggctttt ttctttttcc gcgcgccggg ggcttcgggt
     2941 tcaggcagat aagcatcagg cgggccgaca cggatttccg tcagggtgcc gttctggtcc
     3001 tgagtaaacg tgacttccga gacaagcagt tcggtattgt cgaaaccaca gactggatcg
     3061 aagacaatca cccgctggtt gggctgccac agcgtaccgt taccctgtcg ccagccctgc
     3121 accacatagg tggtttcatc cgtccgcgcc gcccgttgtc gggcttcaaa gtcagcacgc
     3181 gcaatacagc ctgcccccgt ggcctgccct gtctgcctga tatacatcgg acggtaacgg
     3241 gcaataaatg cgtcctctgt gcgggcccgc agcgcggtgg tggtgacctc accgaaatca
     3301 tcgtcgtttc cggcacgctg ccccgccacc tggtaaacag aaaaccgctc ccggatactc
     3361 ttctccgtat cacaggaaag gatgttttcc ccaagtacca gcgcggtatg tgcccgcgtt
     3421 gagccaatac cgccaatcac cagcctgccg tgcggatcgt cgtaagccag cgcctgctgc
     3481 tgaccgagta ttttgttgat aacctcaatc accgtttcac cgtgatcagg ctgaacatca
     3541 ggaataacac ccgacggcgc accgctgttc accacctcaa tgccgaaagg cgcagcaagc
     3601 gcctgcgcaa tctgcaccag cgagcgtccg ttaaactgtg tcggttcggc tgcacagtca
     3661 atcaggtcag ccgtcagact acgtccggca ataccggtgc tgaccgaacg ggcatcgtaa
     3721 cgaacgggag tcgcctccac ccagccggtg atcaccagct catcaccaat cagcacttcc
     3781 acttttgaac cgtttttaat gcgcggctga agcgtggtga taccctcatc tcccggccac
     3841 tggcgagtga tctccacact gaaatcccgc gccagccgtt caataccggc accgatgcgc
     3901 accgatgtcc agccattcca ctcccggcca tttacccgta gcgtgacatt gtcgttcatt
     3961 gcactggcac cttcagaggg atcaccggca caaagccggg atgcgtaatg gcattacgcc
     4021 ggataatgtc cgcgtcacgc gccgcgttat caaaccaggt cgccgccagc accagcgcgg
     4081 gtaaaacctc atccggcgtg cgctgaatga tccgtgcaga ctgttcaagg cgcgtgttga
     4141 tatccgcatt cagatctgct ttcacccggc gcagcgccag aaacagcgca tcactggttg
     4201 tacgggacaa ctccttatca attgccgtat tcagtgtgtc gcgaatgtcg gtcagttctt
     4261 cccacgttgg caggtcaacc gtgtttttca ccgccggtgc attgttcagt gccggatgcg
     4321 tgacagaagg ccagccggtg ctctgcgcgg gtgttgttga ctgccctact gtggcattct
     4381 gcatcaccgc ggaagttgtg ggcgcaggca atcgtgtgac ggcatacgcc gcttcgctga
     4441 ttgcggtcgt acgaagggtg ctggcaacca cgttacgctg ctgcgtcgcc gtggcggtag
     4501 ttttactgtc cgttttccag acgccgcgcg gttgcagatc gctgccgagg ctgacaccgg
     4561 aaagcgtttt gatcatggtg accaggtcgc tggcgttacc ataaaggcgt ttcccggtac
     4621 gccacatttt ctgcacctgc tcaacgaaat ttttgcctga cgatggtggc ggcagaagta
     4681 ccgagatatc cccctgcaac agcctggcgg catccgatac ggcagaatcc accactttca
     4741 tcgcatcaga aacataccca agcattgtgc tggcattacc gacgacgtcg ttctgcacaa
     4801 aatctgccac gccatcgata ctgaaaccac tgaagctgtc actgatgcag tcatccagtg
     4861 cagaacagga tgacatcagc gtctgcgccg tcgccgcacc tgaagtgggg taagagagtt
     4921 ctcccgcttc gacaaacttc aggtcaaagc ggacaatacg cccttcactc ttcgatgtgc
     4981 tgacccgaac ttccccgtca acacagactt tcagctcacc gtaagtcgga tggacaagcg
     5041 tgccgggacc gggtttattc agcgcgtcaa tcaggcgatc gcgctggtca aagcagtcat
     5101 ctcccaccac ataagctgtg atggacgggc ggaaagtgat tttccccagg tcttcggtat
     5161 agggtttgtc gcggttcggg tattcgtgcg tttccacacg acgaccggtt cccgcacttt
     5221 cttcttcaac cttaaacggc acaccgcgaa atgacgcgtc ctgaagtctg tctttccacg
     5281 tcatataaac tccgtacata aaaaatccca ccggagtggg actcattaac agattaattt
     5341 ttcattacct gccaaagcgc gtatagccaa catcatggct gacatcaaaa ccgctggatc
     5401 gcgtttccat aacccgcata cccggaggcg aattcacaaa agagaccttg atctcaccat
     5461 caacttttgg cgcagaagct ttgttaatca tgaagggatt cgggcctgtg gcaccggagg
     5521 cgttgtttga ctgagccgga tctaccgcct gataaggtgt gtatccccgt gccggtattc
     5581 ccgtcccata agcatcataa gcacccgcgc cccactgcgc agagttaatg gcatcgaccg
     5641 tgtcaccgga actgtcggta aaccactcaa taattggctt cagtttgtcc cacatatcct
     5701 gaaaccactt aacaaccggt ccccagttat tgattaccat ccccagcggc gaccaggcaa
     5761 aaaccttctt cagaagttcc cagccagcct caaaataagg accaatggtt tcccagagct
     5821 tcttgaaata aggtccgaca acatcccagt tagtgataat taatcccgca gccagggcta
     5881 tcgccgtcgc aatcattcca atcggcgtca tcgacatgat cctgctgaca atactgatgg
     5941 caccgccaac gcccatcaat cccagtttca gaatcgcaag accggcagca agcccgacga
     6001 cgccgcgaat aacccgggga ttttcatccg caaacttcgt gaatttttcc cctaactccc
     6061 ccagccattg cgtgatattt ttggcgtcac cagaaaatgc gccgccaata gccgcaaggc
     6121 cgttagttgc ggtccccgtc attgcctccc acaggttgga cagcgtacca agctgggcct
     6181 gaacacgttt attcaggctg gcctgtttat tcatcttctg ctggatctga tcgtagccat
     6241 cctttccttt atcgattagt gcattgacca cctgaagggt ttcggcatca tcaccaaata
     6301 ttgccttaag tacacctgtt cgcttaacgt cggtcagttt tcgcagcttt gccagttgcc
     6361 tgaacatgtt atcaagaccg ccaaaacttc ctttgccgtc agtaaaatcg agctgtaccc
     6421 cgagtttctg gcgggccatg attttattga cgtccctgat tttcttaacg cttaatccgg
     6481 actggataac ttttcgcagg gcgttacctg ccgactcccc gttcatcccc atctgatcca
     6541 tcatgacgct gatgggggca aggctctgtg cagcctgaag accgtccttg ttcaccatct
     6601 tcagaacaga gctggtttta gtgaagaagg acaacatgtt ggtatcgtca acacccagat
     6661 aaaacgcctt ctggatagtg tcgaacagcc ccatcatgtc ttctgacgcc gttccggtag
     6721 catcctgcat ctttgcagca aactcagcag ccgcttccgg tgtttttttc agttgtaccg
     6781 caagataagc tgtcgcttta cccacaccgc caagaatgtt ttctgccggg atcccctgac
     6841 gcaccagcat ctgcatcatg ttctggaaat cagccgttgt accgggtagc tggttaccca
     6901 ggccaatagc cagtttattg atgtcctgaa agctctttcc aacctcgccg ttcgcatcca
     6961 tcatggcgac tttcagcccg gtggcggcgt tttcctgatc ggcataagat ttcagggaaa
     7021 gcgtcagacc cgctgccagt ccgccaccaa gcgccagccc accctgtgac gcttcttccg
     7081 cctggcgttt aaatccccgg attttctttt gcattttcga cagcgcggga gaaagcctgt
     7141 cgacaccggt gatcaacgcc ttaagctcaa attccgccat gtgtgcgttt ctcctgctct
     7201 atcctgtttg cctgactgac cagcaaggga atttcactga tcggcatatt cagcaattcg
     7261 aaaggattaa tgcgccagta gctggcgcag tcaaagaagc gatcagtgag gtattcagcc
     7321 gtcaggcctg gaggaaaaaa ccagccacaa gccacgccgc tgcattcagg tctgccggag
     7381 acatctggtc gacagagctt tgcggcactt tcgccagccg cacaatgtat ttcgacacca
     7441 catgcgccag aagtctgact gactcatcct gattcatctg gtagggatac cccagctcgc
     7501 ggacatcctt cccggtgggt tcatcaaact ccagtacgga gagtgtctcg ccatgagcag
     7561 taatcggttt ctttaactca agctctttca ttactggtaa tccccttctt caccgtggaa
     7621 ctcaagatca accgtgcctt cttcggcatt atggttcgct tcgccgtgca gccaggcaga
     7681 cgacagtaca tagacctgac cgttcgccag ctcggcagtg atggtcatct catcagacga
     7741 ggtgattttg cttaccggaa aattcttcgg caccttgaag gtccctttga cataaggcgc
     7801 acggtgagtt tccttgcggt ccactgaacc gtccaggccg atgatgtcat cgttaaccgt
     7861 tttgttcatg ggcacctcaa tgccgccggt cagcgatagc tgctgaccgt caattttgaa
     7921 ataacaggtt cccccgatac gggccattat gcagactcct ctgaatactg aagacggaac
     7981 tgattaacca cggcaaagac acgcagctgg ttaacatagt caggcgggaa cagcgtgttc
     8041 aggcggttcg gatcgctggc atcacgctcc acaaccaggt actgcttaaa cagttcgtag
     8101 ttttccacga tccccgcacg ctcaagctga cggtaggttg ccagcagttc ccctttgatc
     8161 accgccgggg tgacaatcgc ctgaccggga ccaaagcggg taccgtcact ggcaagcttg
     8221 tgacgcccgt acttactggt aatgacggat ttcagtttgc gcagtacata cgcgctggta
     8281 tgcagcgtct cactgtcgag gtaactgtta tccgcaaccc cgtaagcgtt tttcctgtac
     8341 gtggtgacat cacgctgaat gcgtagtacc ccgctttcga catacgccgt tgccacgcca
     8401 tgagacagca gggtctgttg ttcggtcatc gtgaaccgtt tccccttcgg cgcaggcagc
     8461 atacccacca gctcaccggt ctgcgtggga cgtgccggat cgttgcggat aaacaccgct
     8521 gcgcgggcgg tacggcttgc cgccagctcg tcggcaggcg tctgggtctc tttttcgtac
     8581 cccgccaggg taatgtgctg ctggttaaac tggtcacctg cggtcaccag ttctgacagc
     8641 gtgccgatct ttgccgtata cacatgacca tacagctgac gcgcatagct ccagcgaccg
     8701 ctggtatcgt tcatctcggt caccagcgtg ttaacggagg ccgtgtcgtt gaacggcagg
     8761 ccgatataat caaacggctc atccgccatt gcagccaccg cgccggtgag aaccggagca
     8821 cccgttccgg cggtacccgt cgccacggca atctgtacgc ccgctggcag cacttcgccc
     8881 ccaccaaagc cgtagtaatt gaggctgaca ggaatttcat tcccacaaag ccccttatga
     8941 cgcgcggtca gtgtgaccac gcctgccaaa gatgaagccg taaacggcag ggtcggaacg
     9001 gcattgatgg catcctggat actgctggca atcatcgtga cgttatcgcc gttagtcacc
     9061 ggtgcctgca cgcgggtacg tcctacatac acattcaccg tgccggtttc ggttgccgcc
     9121 ccggtcaccg tcagcgtaac tgttgccgcc gcacctgtgg attcaggaac ggcaatcaca
     9181 tacagctcgc caaacgggtc agtctggcga taagcctcga ccatacgcgc cagctgactt
     9241 cccgcaccac aaatctggcg tgcatagtct gccgacgaca tcagtaccag actgttggca
     9301 acaatctctg caccgttatt ggcatgacca atcagcagcg atgctccgct gtcctgtgca
     9361 gtattcgccg cctggttatc catttccgca taaaacaacg gaaccagcgt attcgacgga
     9421 atggtgttaa agcttatcgt catcggtgtt caccttttta ttcacgcgcc ggatatcacc
     9481 cgctgcttca cggcgcagcc agtagttgtt ctcgtcaaca tttcgccctt cggcgggcaa
     9541 aaggtcgccg cgggcagggt caggcactga ccgcccttta acaggtttga caaacatgag
     9601 gatcctcagg aaggaagggt tatttcggtg tgatgttcga tatcgccgtc aggcccgtta
     9661 ccgggctcga gataatcaac atcaatcgcc agcgtttgca gttcatccag actgttcaga
     9721 tcatcctgct ggcgggtatc gtcttcagtc agctcgctga tgaccgaaaa atcgaactga
     9781 taaatcagct catgacgatt cagatccagc agcgtgccgc cgtcataggt aatcgggtta
     9841 ccgcacgctt ccgggttcca gcccagcaag gccttaaaga gcatctgccg gacatcgtcc
     9901 accacatcat acgaagcaaa ctgaccgagc tcatcacgcc cgttactcag tatgacaacc
     9961 acggagaagc cctctttcag ctcctgccag tagtcggtct ggcttttgtt ttctcccgga
    10021 gagtcatcac ccggtaccac atacgccgcc gggagtctca gctttccgac ctccggcaga
    10081 tttttgaact gtgccgcgcc tgccacccgg ttttcaaaat acgggcagcg ggcacgcagc
    10141 gcagcaataa caggcgtcag tttcatctgc gtcgtcgctc aggcttcagt gatttacgta
    10201 attctcgcgc cagaaaatag cgtgtccagc tgcggttctt ttcaagcgtt tccaccataa
    10261 agttattacg tggagccagc cgccagccgc tgccaccgga tgcaccacga tgatgactac
    10321 gacgacgttt tgctcctccc cggacaccaa aaaacagaaa cgccggatag aagtcaccag
    10381 agatcatccg gttccccttc ccgttgcgct ggttaggggc aatgcgtgtc ataaaaccgg
    10441 ctcgcttttt actggctctc ggcaccatgt aaccaatcga acgagccagg cgtccggtct
    10501 gataaccggg gttttcaccc ggtgccgacc gcgcacggcg catcaccagc cgacgggcat
    10561 cacgcatatg acgctgccca atcgtgacaa acgcccgccg gacacgggcg cggttaaagc
    10621 gcatctcggc gggctgctga acatcaacgt gaaaaaaggg agtcgccatt gctgcctccg
    10681 tgactctgcg taaattcgcc cagttccgta cactccagca gcagaaagcg ccgcgccccg
    10741 ttcagatcgc gctgacgttt cacccggtac acactgtcat cacagaccac ctcataatca
    10801 gcagtgatcc cgcggcggta gcgaatggtg atgtaatggg tgatggcgtc cccggtctgc
    10861 gcggtttcct gccaggtggt gtatagcgcg gcgatttttt tgataccgat ttgcatttca
    10921 tttgataccg ctgaaaaacg ccgatacgca acttatttga tactaaatcg gcgcttttct
    10981 tttgggtttt agaatgagtt tttaagcgtt cactcactct cttttaaacc agttttaact
    11041 gtttgtggtt ttgggtaagg ctggactttg aatagctttg tgttgaggcg ttttctggcc
    11101 ctcttgttca gaaaccggat gtaacgaaac tgacggaatt tatgaacact ggctctgtcg
    11161 atattagccc tcagatgttc acctcgctgt ccgcctcgtt taatggcatt cctgcaaatc
    11221 tcgtgatacc actcgccatc aagttcatag aatgttgatt cgtggctgcc tacataatca
    11281 aaattgcttg cctgatacac gacaccaaga cagccacaac gctcatctgc aaacgactga
    11341 acccactgta cctgcggata aagctgcctg attagcttga gcgcgtaact gattgccctt
    11401 gattcggagt ttctcggcat acagtcatgc agccataacc ggttaagctc catatattca
    11461 cggttccgcg tgcccgttac gacgcgtgca ccactgtttg gattaagggc atagccccat
    11521 tgcattacgc caaccagctc ccgttctgaa aatatgccca gatgaaggta ggagttattt
    11581 acgaaacggt ggctgtaatg tttattgacg atgaccagtc tggccagcca gcaacttatt
    11641 gtctcaaccc gcaactcacg ggagccataa ccaacgatat tgtcgttata gcgaataagt
    11701 tcaggcgtgc tgatgatacg ggatgtgact tgctttctgt tccccacgac aggatttcct
    11761 tgtgtatgtg gggtgctctg tggcgctcgg agatgtgatt tgattgaggg ttttacagcg
    11821 cggacatttt acttccagat aactgaaact ggcccgtgcc agtagcttat tgcagtgtcg
    11881 gcaccgtatg tttcgataca tggccgcata acctcctgct ttgttgttga tgcctttctc
    11941 atgcaatcga tcgacaaaaa cgatcgattc gatttgttta attgatacaa caaatagatt
    12001 gaaaagtcat caacaatgat tcagggaggt gattatttac ttaaagaaaa cgaagagtta
    12061 ggtggtgaaa acgatcataa aatgatcatt ttattgactt tttatacagt gaaggtagga
    12121 tcgcgcgtat atgtagtatg tttcaaatca acaggatact acatacataa agggcgaccc
    12181 agatagcgcg aactattctg ggccgattta gacaattttg catcaatgct ttacttcgag
    12241 ctcgtggcgt atcctatgat attccccggt ctcctgtaaa gcagagatgc ataaaaagga
    12301 gatctttatc atggataaag agcctgtttg ccctgcctgt aagactccct tacagctcat
    12361 ctatcgccgt tctcgatctc aaaatggcaa gacatacaaa ccaaaaaatg cttcctgttt
    12421 caggatgctg atatgtcctg cctgttctcc ccggagggaa aatacacaaa acgtgcatta
    12481 acaaattcac ccggcaggga aacctgccgg tcggtattgt tacatcatag caccgtcagt
    12541 tccgggctga accggctctg gttgctctac gggttgaggc ttattttccg tgctgttttc
    12601 agccggagaa cggccaacca ttcgggccag cacttcatca acatatgcgt cgatttgtgc
    12661 ctcaaaatcc ttgcgaacct gtgccttcag cacgcggttc acttcttcag aataaagtgc
    12721 ctgtttgaca agctcttcag taaccgtgcc tttaatctct ggcatgaata ttctcctttt
    12781 gcaggtatat tgccccgtta tcagagcggg gtcgggtcgt ttggggatga cggtgcctgt
    12841 gctgttttac tgagtttttc agctgccgca tcatcaattt tacggcgaat ataatttctg
    12901 atgaccttat aaccgccgct caccagatat aacgcactga ccactgcgca gaaataaagc
    12961 agcattgtat gaacgaatgt catataacct tacctttgat attgactaca taattcgtta
    13021 tcgggtaaaa acatcgaagc gactgataac cactccttcg ggagtatttt tattttcctt
    13081 tatgggttag gtagctcccg ccagccatat catgaccggc gggatttttt tattctgtta
    13141 ccgctttttt cacttccacc acaatgctgt caatcagcac cgggtaagtt gcattttttg
    13201 tgatatccgt tacgcggagt ttgtccaccg ggaaagttcc tactggtgac tgcgtcaggg
    13261 taaacgcggc accatcctga ccatcaataa ccggaataac ctgaaggctg ttatttcctg
    13321 caaaacggaa ggccagcgta tgccattcat tatcaaatgc accaaaggtt cccagtttct
    13381 ggttatttgt tgccactttt gcattgtggt acatcacatt caggtctttt gcgtctgtct
    13441 ggatgtagaa cgatgccagc agattattcc ctgcatcccc tgtcagggtg acaccctgcg
    13501 gcagagagga aaccggccag taaagcgcca tgacatactg gtttgccacc aggtcgcccg
    13561 gtactttaaa acgacaactg attaaaccac catttttcag cagttcagaa ccagtaccgg
    13621 catcatgatc cagataccag gatgatttac ctgtttcctt ggtcagtttc attgccttac
    13681 cacctgtgcc tcctgcatca ctgataattt cagctttacc gccagccggc tcccagccct
    13741 gggctttcag atttccttct gactcactga cacggtaaga cagcagtgtt gtggttactg
    13801 cttcagtttc tgaaggtgac ggcactggtg cttcagtttc agaagatgga acatcggctt
    13861 ttactgtggt tcgccccgca tgagtcagaa ctgccgtggc cagacggtcg gaaataatcc
    13921 cacgacgtgc ccatgaactg aaatggctgg cccggtccgc agatgtccag gtggcggcac
    13981 tgttacgcca cgttgagccg taataaccaa tggattcaat atccgggtct tctgccggat
    14041 tgttggtggg tacattcacg ccattttcat ctgtcattaa tggaacgaaa tgaatgtttt
    14101 tatctgtttt gtttttgtag ccgccataga ttgcctggta tgccggttca tttttctgtt
    14161 tccagaaata cgtggtatcg ccacaaatcc acggaacgcc agcagcactg ccaccggtac
    14221 actgttccgc ctgttcagcc aggtctgcgc ggaatttttc caccatcgcc agaaacagcg
    14281 cagaatgctc cgtcggcttc gcatcaatat caaactcgcc ctgcatccac accacagcaa
    14341 gcaggcggtt tttcgggttc ttcgccagtg ccgcttttgt ccggctgatt aagtccttat
    14401 acagcggctt atccacaccc cagcgggctg agtcctctga agcacccgtg ctgtcactgt
    14461 acgtgccgtc agcacccacc gtgaatgcag aaccaccacg gcagcaggga accagcagaa
    14521 tccccgcatc tgccggaata aacggcagca gttttttggc aatgtgaagc ccctgaccca
    14581 cggtgccata ttgcccttta gccacatccg ctttcgggtg attttttcca ctcatatcct
    14641 gcacatcatg cagacagtgg tcggccagaa tgatgtcgtt atactgacaa gctttacctc
    14701 ccggcgtgac tgtactgcga cgggctaact gcataatacg cggttcgggg cggtcatatg
    14761 tctccggcag tggcagacct tcaccgtaag ccataccgtt tgactgacca gcaagaacca
    14821 ccacgtaata ccaggaaggg gagctggaag cagtttcatt tcctgacgga cgggtatgtg
    14881 tttcagcatt atcccagtct gacggttcag cagtaccggc aacatcagca cttaacggaa
    14941 tgaccgcacc aatacccgct ccggtaattt ccacactcgg atcaccggat actggcctta
    15001 cccacaggca atcccgggta ttaaaactga tgagacagtc agcaaatgtc attcctcctt
    15061 ttgtttcggc cggaggaaag ggagcctcaa gaaatgccgc tgtaccaccg gaaactttaa
    15121 cagtaaagtt tccgggcatt gatgatagtt gaacccattt ggacgtcata ataatcagcc
    15181 tgttcctgaa taaactgaat tatcatatcc acagaccgat aaacgccatt ttgcgttaat
    15241 tcagtaaagg cccaacaaag agagtgtctt atttttccga aacgctcgca ggataataac
    15301 gataaagggt tgaaagaccg atatcaaaaa taagtgccac ctgtcttcga ctgacacccc
    15361 cggccagtaa acgcccgacc tgctcgtatt gttcctgcgt gagacggcgg ggtctgccgc
    15421 cggttcgtcc cctttcacgg gcagctgcaa gaccagcccg tgtccgttcg acaattaatt
    15481 cacgctccat ttctgccagc gcacccatta tgtgaaaaaa gaagcgcccc atgggggttg
    15541 acgtatcaat gttgtcagta agactacgaa aatttatccc ctgttgctgt aattcatctg
    15601 tcagcgcaac aaggtggcgc atactgcgcc ccagcctgtc cagtttccag accatcagcg
    15661 tgtcaccggg ttttagtttc ttcagtgccc gcttcagtcc cggccgctgt gtcgttttac
    15721 cactcatttt gtcttcaaaa atctgctcac atcctgcaca ttccagcgca tttctttgta
    15781 aaccggtgtt ctggtcattt gttgatacgc gtatatagcc aatcagcatg tatttacctt
    15841 ccttaaaaag aggtggaaaa gccttttaaa gcagatatac ggcatccctg aaacctcggt
    15901 ttacgggata cggtaaacag ggccgcgaat gccctgccat caaacggtac ggctgttgca
    15961 gcaagcaggc tggcaaatgc ctgcagaatc aatggtgtcg cctttgacgg cacgcaggat
    16021 ataaacatta cgtcaggaat gccggaatca acggccaatc agcgttatgt tacaggggta
    16081 agactgggag cgcaggcatt aagcggcgga ctggaatata attactccct gtcatcagga
    16141 aatgtgatta ccggttttaa gactgacgga gactgggaaa tgcgtggtgg tgatgaccgt
    16201 gtttattaca gacagataca atattgtatt aatgggcatt gggtatccgc tgcaagtatt
    16261 taattaacgg gggcatagca cacagcagcc agcatctgaa atatgcgtta tttcagacaa
    16321 gcattgcctg tatatggcga tagtattatt cctgttttta tcattgattt acatggtgat
    16381 gtttatgcaa ctgattaatg tcaaacgata ttacccgaaa gacaaaccct atggtgagga
    16441 tgttcagtat ttccagagtg aggacggaag ggatttttat gaatctatcc cgctttttac
    16501 aaagaaatac aaactctgta tttcgcctgt aacgggtatt atctgctctg ttgctgaaga
    16561 tgtctctgct ttatatccgg caggttttac tgttgttgaa gtggacgaac tgccggaagg
    16621 tgtgaatatt gacggtaact ggcagttttc tgatggcctg atatcaaagg tgccggtaaa
    16681 ctggaagacg gttgctgaaa agcgtcgctc atcgctgctc caggaagcca acgaaaccgt
    16741 ggatgactgg aaaacagaat taaaactgga catgattagc gatgaaaata aattacagct
    16801 gacgcgctgg atggcgtata tccgacagct taaagaaatg cactttaatg atattgccag
    16861 tgaggggcat taccaggcga ttccctggcc ggaaaaaccg gaataattca gagggtgcat
    16921 tcagcaccct tttctgtggt ttacactgaa cttaccgttc tccaggcatt attaatataa
    16981 atctgaaccg ggcgataata gaccccaccg atattgtccg cagaccggga gccggtttcc
    17041 tgcacgtcaa taccggaaag catacaaccg gaaggtgcgc tgtaattcca tgacatttca
    17101 ttgcctccgg gtgaatgata gctctgtgct ccaagctgaa cattctgtac ataccggcca
    17161 tctgcggttg actgcgtcat tcctgacgtg atgttgatat ccagcgttcc gtcaaaagga
    17221 acaccattaa tatttcttgg ggtggcgagt ctgtttgctg ccacggcggt tccagccgat
    17281 ggcagggcat cagcagcctt atttaccgtt tcccgtaaac cgaggttatc gataaaggtt
    17341 cctttgtcct ggatatccgc gccattctgg tccctgcgca ggtaacggtt atcaccttcg
    17401 ctcctgctgt aaacatccag attattacgg gcggtttccg cattctccag gtcggcaagg
    17461 ttctgatcct tcgccagggc attaacgtca gcgggaccaa gactgttttt tgtggcaagc
    17521 gtccccagac caagataacc acgggccaga cgctgggctt ccgcaccggc atcagcaatt
    17581 tctttcaggt tgtttttccg ggaaaaggca ttcgcagcga taatggcttt aatggacagc
    17641 gtaagctggt ttaactgcgc cttatccggc tgaatactgg cttcagccag aatattcagc
    17701 agttcggcct ggagaatatt cagccagtcc tgaccaatcc agctgatacc tttttgtccg
    17761 tcaccttcgg taaaccaggt ggtggtatta ctctgcgccg gagccagttc aggcatattc
    17821 gccacgccgg aattattatc aacatggaac attaaatatt ctcctcttcg tgactgtttg
    17881 catatacgta aataaacgtc tgccatgcag gcttataacg gttcagaata cattccagcg
    17941 caccaccctc ataaatacgc agcggggtga gaatatcatc cagcacattc atattccggt
    18001 agccggtttc actgtttatc gtgacaatac tgacccactg tgaatccggt gatggctgaa
    18061 tatctatttc ataaccaaac tctgccgcaa accggatata aaattcacgg ttaagagagg
    18121 gtttcatccg gtatttattc cctgcataac gctgacgttc ctgaatggtt gcgccggtca
    18181 tatcacattc cggtaacccc agataacgct cccagtcttc cagcagcaga cgggatgaat
    18241 caggaaaacg ttctgccagc atctggttac cggtccagga aacacgctcc gtggaatgac
    18301 tgaggcccag gcacaaagct gcaagaacgg atgacggccg cttatcccat gccagcccgt
    18361 caggcagcaa ctgcaacagg gcgcgctgat gcggggtcag ggttacagcc atgtgatttc
    18421 ccccacagtc agcaactctg tatctccggc catcacggac ttcagcggac tgcgaacttc
    18481 aaaatcctcc agtcctttca cccctgcaac agcccgccag aatgatgaag gcaatacaag
    18541 tccacccggc cgcgattcgt tataaagcag gtccgttaat gcctgtttta cggcagcctg
    18601 attttccggc gttttgggta caatcctgat ctcaaacgcc accggcttat tggtcagctt
    18661 aaacacgctg attgtcggcc caagtggctg accaacgggc tggcccgtcg ccggatcatc
    18721 atgactgcgg atataatccg ccacccgctt cacatcacct tctccgggga aaatgtcagg
    18781 gttattatcc tgaacaaaag tcacaccaac actacccgcc tcaggccatt caggcttaca
    18841 ccatgcccgc gtcacgcccg gtacttcccg tgcccagcgt tcaaaatcat actgtgttcc
    18901 ccctgatggc gggttctgta cccggaatac cagcctggac agcaactccg gcaccgtttc
    18961 cacatccgca ccaccggtga gtcccgtgcc ggtaaccgtg gctgtctggt tgatgcccgc
    19021 ctgtggcgtg ataaaggaca ggcgggttcc ggtcggggta tttccggcgc gccccgcact
    19081 ttccgcctcc acattaacat tcagtgttcc ggcttttccg gtcgcagagc cggtgatgcg
    19141 gtacacaaca ccatcgcttc gctgaaggag tacgccttcc gtgatccctg catccgtggt
    19201 cagcatcagt tgtaccggtc cgtcagcgcg ggaagccggt ttacggatga caccccagaa
    19261 tgcgcagtgt ttcaggagtt cggcttcatc agcttctgtc ggaattatct gccgggagat
    19321 ccaggccaga tgttcatgct cctgtgctga cagacccgcc agtgcatacg caatggcatt
    19381 cagggtggtt tcattcacgc ccggctgcga accgggcagg cgctggctga tatcctgttg
    19441 tgtctgtgtg atcagttgcg ccagtggtaa gggctgataa ggcatcattc ccccttcata
    19501 tcagcataaa aaatcatcgg atgtctgctt ccgtccggca gggtgatttt cacggaaagc
    19561 gaaaggcgat catgccccac acgcatggca tgacatacca cggatgtggc aacaccgctt
    19621 ttatgcagcc acgccagcgc ttcatcagca taggcctgtg cacggctgac cacggaggac
    19681 agcgtctttt cacggcttaa cagccagaga cgggagccaa tggggcgctc ccggaaactg
    19741 tctccccacc atccccgacg gtcacgggtg ccgtcaggga tttcatcgga atccagcgcc
    19801 cgcctgtccg taaacagtga aataatgacc gcagtcagca ggctgttatc cgtcagaaga
    19861 tctgcgccgt tcagttgcag ggaaccacaa ccctgatccc agacgacagc aatatcagcc
    19921 attctctggt cctcccgttt tgctgccttc accgttatcg tgatgcacat gacctgaata
    19981 ggtgatcccg gcaataaccg cttcggacat cgtgaacgcc ccctgtgatc ttccggtgcc
    20041 gtcaatggac acattgcctt tgacatgcag gttcttatcc actgtgatat taccggtaaa
    20101 gtgagcctcc ggtgtatcca cctgaacacc ttcatcggcg taaatctcca gcgtcttaca
    20161 ggtcacaatg atccggccgt tctttgtcag acgtatacgg tgcccctcat ggtgatacac
    20221 gccggtatct ccggcggtaa gtccggtcgg gcgactgcgg cggtcttcca ccacaagcac
    20281 cacggcctga tcccgttgtc cgccagcaca ggccagaaaa acttccgcac caggcagggg
    20341 gacgctgatt tgcccgtact gctgggggcg ttcaacgtca tcaaaggttt caccatccat
    20401 accggtgatt tgcacattct ggattttcag cgtgtccgtt gtgccggtca gtacaccacg
    20461 cccgaacagc agacgaacac cccgcatcac gggggccaga aggcggctga tgacttcgtc
    20521 attcatcatt gaatttaatc ccctgttttt tcatctgtgc ccgcacgaag gcgtccacgt
    20581 cacccgcatt tccgcttccg ctgtttcccg gctctgccgg aacaataaag ccgtcacggg
    20641 gtgccagaat cagacgggtg gtttcgccgt gatttgcatc cagcgaaaac tccacctgac
    20701 agacaagaag atcacgctct tccactccgg tgcggggagc cgtgacagcc gtcagcaggt
    20761 tgatatccca caattggccg ttctcccgaa accagtgccg cacggtggct gtcaggcgca
    20821 cagagcgggc aaccgcccgg cgcatttccc ggacagccct ctggcgtgca ccgtctgcat
    20881 caacagcatg atccgccagc acaattttcg gtctgtaacg gtggatggca ctgtctgtca
    20941 ccgtaccaac tggtgcggcc agcgttccgg ctgtcagtgc atccccggca tgaccaccac
    21001 cacgcccatg cccccgtaca cggtattcac tgtggcgcag acggtgatcc acgttgtgat
    21061 ccaaatccag cagattttcg cccagcgtca gcgtgtcgcc acgctgactg ccggcctgag
    21121 tgaaaaccag ttcaccggcg gcattactgg ttaccaggac gcctctgtgt ctcgcggccc
    21181 gtgtcagcgc atctgccacg gtttctgaat tttccagtgt gaaggtggaa aagggccggg
    21241 ctgccgttgc atcatttacc tgccagcgca ccgtgacccc gaacggagca cacaaatctg
    21301 aagcaatgtg ctccagcgtg cggttcctcc actggctgcc cggatgaatg gctgaacagt
    21361 ccaccaggtc accggtttta tcccgtccgc tgagcgtgat ctgaaaacgc gtggcgctaa
    21421 tgcgctgccg gacctgatcc agccagccag tgatcacagg ctgaccgtca atgcgcaatt
    21481 ccagagactg accggcacgg acggatgacg gaaggcgtac accgggcatc attaccccca
    21541 gttcaaagga gcccgccaga tgttcaagag agcgacgaac actcaccgtc agccagccgg
    21601 aaaaaatctc accgcccaga tacagttcaa ccctgctgct cactaatcac ctccactgaa
    21661 tggcctccgg gaatgaacag cggatcaaca ataccgttac ggcgcacaaa acgttgccag
    21721 ccggtactgt tcccggtggc acggtacagg gtcaccagtg caggctcggt ggtacgtaca
    21781 gtcactactg ttgcaccggg tagctgaact ccccgtgtat tcagatcctc tgtcagtgcc
    21841 agtctggcat cacgaagcgt cagcgccgtg gcggtatacc cctgcccgga cagtgtcatc
    21901 acctgccgtt cgagcgcttc gcttaagtca cggttaatgc gctgaagatc aactgaactt
    21961 tccagccaga caggccaggt acggtgggct gcgtcactgt ccggcagggt ttcctgactg
    22021 agaacctgat ccagcagttt tcccacggtc tgtgcctggg ccaccacggc agcactctgc
    22081 attaccgccc gtatcagacg aatattcttt tgtgctgccg gtgtcagacc gatgagagtc
    22141 tgcggattat cctgctcatc cagcacgtaa cgcagggaag ataatgtcct gtacagttgt
    22201 ggcattccct gagagacggc ggatgtcgtc cggccgatac tgccaccggg ccgggtattc
    22261 aggcctcctg aagacagtga cggcagcgat ggcagggtga tcagggcact gaaaagcccc
    22321 gcaaaagatg aggccatgcg ggaaggtgct gtcagtaatg ccgtggcatt ccctttcagc
    22381 gccgtgaacg tggcagtaaa ggcactgata tcctgtacaa tccccatgcc ggaaacggca
    22441 ttttccaggg cggacacctt atcactgatg gtttcggtca ttgcctggac atcgtgcatt
    22501 ccgtcagaga tcaccgtcca gcccgcttcc agggtttcaa aaacctgccc gagcgcggtg
    22561 gtactcttct gctccagaat ggcggccgtg tcctgtgtga cagcaggtgc ggtatcatca
    22621 cttaccggcg tgacattaat ggtgaactca accacgccct gttcagcggc gttataacga
    22681 ctttcaaagc tgtttatcag cacatttaac gtgccgtaat ccgggtgaag cagctccccg
    22741 gcaccgggag cacgaagggc atcacgcaga cgatttcgct gcgtctgaac atcatccccc
    22801 atcacaagta cactgaacgt aaattcagcc agtttaggtc ccagatcgtc tgccccaccg
    22861 gtttcccgta acggatattc ccgacggaca atgtttcgcc cgccacgttc gcgctgctca
    22921 cgccagacga ggaacggcac accacggaac gcaccacgac cgtcaccaga ggccataatt
    22981 ccctccgttc cagacattca catccagtcc gtgaccggac gtgtcatcaa tatcaatact
    23041 gcgagcctgc cacccttccg gggcaaccag ctccacacgg gcagaggctt tttgtaccgc
    23101 ctgtccactg ctttcatcat cactgccccg taatttctgc caggcttcag tcagccatcc
    23161 ccccagatag ttaccgagat agctgcccac cgtagaacca atggcaatcc ccacaggtcc
    23221 ggctgccgcc cccagcgcac caccggcaat actgcctgca agagagccga ccgcttctcc
    23281 cttgtctgtg gcactggcgc tgccatccag cagaaccggg gctgccatcg cgccagccca
    23341 cagggcaccg ccacccagcc ggccggccag tccaccggca ctgcggaaaa agccggagaa
    23401 acgccccagc cccatgcggc taccgacaga cgaaagtaac ccaccggcgc gccccatcat
    23461 gcgcccccag aaccctgccc caccagaaga gggagacggt ggcactgggg acggtggcag
    23521 gggaggcgtg accggtcttc cgcgccccgg tccgcgtttc ctgcggcccc gcccctgacg
    23581 tccgcctgag gaagtatcgg tcgcgccggt gccggacagt cctcctgtgg gccagtttgt
    23641 tacaagtact ggctgaacgg ctgccggatt taccccggta agaaagttta taaaacgttg
    23701 ccctcgtccc tgagatgtgc ctggtggtgt tccggccgga ggagagcgcc ggaaaggtga
    23761 agtcaggaag gccgccgctg ataatccgta tcccagcaat gcagcacccg gacgtataat
    23821 ccccattccg acagcccgcg ccattttcag ggcacgggtg gcgagataca tgtacagcag
    23881 gtatttcgcg gcggtctgtg ccccctgacc aatgcgatcc agcgcatcac catagcccgc
    23941 atcccgcaga gcctgaagcg tgtcgcgtac ctttctgatc gcctgataaa accctgtcac
    24001 tgcatcacgg gcatactcaa agccctggtt catggctgat gccgtgcttt ccgcctggga
    24061 atggtaactg ccatcagatt tggcactgtc aacccatgaa agaaatcccc tgagattacc
    24121 cttcagtttt tcaaatggcc cactgtccat cacatcccgg gcaaactgat cccagacgtc
    24181 acccatcatg gccgtcaggc cactccagga attcattgcg tccttttgtg ctcccctggc
    24241 ctgttccgcc agcgtctgaa ataacaggcg aatactgtcc ggcccaagca gccctttttc
    24301 accaagtttt ctgaccactt tctggtccac acccagacga tcagccagca cgcggtaggc
    24361 attaatgccg taggtggcaa gaatatttgc atccgccgcc tgaatactgc ctcttgcata
    24421 catctgtttc agttgcaggg atgccccctg tgcatcggac agtgaccagc cgcccacggc
    24481 accctgatcc tgaagcatgg tgacaaaatt tcgggcctcc ctgtcactca taccaaagcc
    24541 cagactggac gtatattcct gcatgacgcc agccagcccc caggtggttt ccttcgcatt
    24601 ctgtattgcc cacctgcgga catcatcagt tttcgcccga tcccctttgt aaagggagtt
    24661 caggcgaatc atataggttt ccatctccgc tgccgggcga ataaatcccc ggttaaagcc
    24721 atatacagca gcaccaccgg ccagcattcc gtaaagattg ctgatccgcc cgatggtgcc
    24781 ggtaatactg ccctgaagac ggtcaaaatc agaagtgaca tggcggatac tgccccgcac
    24841 cccggccagc gtgcgctgca tcctgcttcc gagtacatct gtttcctgcc cggcacggcg
    24901 ggccgcattt cccagaccac ccagccccgc ctggccggag cgtgagaatg cccccagctc
    24961 ctgagaccac tggcgggatt tggctgagat attgccgagc aaatcaacta tcagagatgc
    25021 tctgagattt cgggccatac ttactgcttc gttctgatga ttttttctgt ctgtcggcag
    25081 tgccggtaaa gttgcgacag ggggaggtta agcgcccatt ccgggccact ttttgtcacc
    25141 atccccagga taattgccgc ttcttcaatc tgatcccgac actgcgcctg atcgcccccg
    25201 gtcagctgcc agctttccgg caagcgcggt atccagcagg ctgaccgctg ccatcaggcg
    25261 ggagagatcg cgctcgctta actgtccaat ctgacggggt gacaacggac cttcaatgtc
    25321 tcccactgcg gcaatctgac ggcgcagcag tgcaacaccg cgtaacgacg gagacgctat
    25381 cagcatcggg ccattgtttg tctccaccac gcgctcggct tctgcttctg cgtcaatact
    25441 gtctttcgct gtcagttccc ggaacgtgac acgatattca cgggtctcac caaacggcag
    25501 accatcaagc agatccacat atccctttgc cagttgctct gtcagcccgg cagtgcgtgg
    25561 atcagccccc tcaagtgctt cccggattgc ctccatcacg gctgcctcgg aaactgccgt
    25621 ttttttctta cgtgtggtca ttttattttt cctgatcact gaacacgggt actggtggcg
    25681 ctggcgaact tcacggagat ctccccgcca ccgtcaagcg aggccggttc actgctccag
    25741 gccttcgtca tcatgtggac ttcgccggta tccgccacaa actcgatagt gacagcagtc
    25801 cagttgttga tttcatcggc ggctggtgaa ccttctccgc ctgccggaaa tttgcagtcc
    25861 agcgtcgctt cacgtggttt ctggcggtaa ccatagactt tcgcgccttt caccacttca
    25921 cgttcaaaac ctgacggaga aaatgtagcc ccctcaagcg tctcgtattc ctgaccgttc
    25981 acacggatag tggccgtgcc ctgatactgt tttccgctca tgccttacct cacaaaatga
    26041 aacggatctg tgctgccagg aagcggaact ggttaaccag atccggcgtg cataacacgt
    26101 caagacggtt tcgatctgac gcattacgct ccacaagcag attcttttta aaggtgtcca
    26161 gattttcgac cagcccgagt tccacccact cttcacccag cgaaattaac tgaagcttca
    26221 tgatctccgg cgtcacgata ttctgaccgg cacgaacggg cgtcccgtca tcagccagtt
    26281 tatggcgcgg aaaacgttgt gtgataaaag tacgcaggga ataacgcagc caggagaggg
    26341 tgtagatggt ttccacatcc agatagctgg gatcactttc gccgtacacg ttctggcgat
    26401 acatggtcac ctgacgttcg atctgaacca catcaccggc agccacactg accgtggcaa
    26461 tccctcccca cagcagactg ttacgctctt cacgcgtcag acgatcggcc ggggccgggg
    26521 ccatgcgtga cggaagcgcc agcgtctgaa gcggacgcgc aggatctgcc cccagagacg
    26581 gcgcacaggt ggcgcagacc gccgctgccc agaggtaatc cggttcaggg gctttgggaa
    26641 tgaatgtgca ggtcgccaga aaatcattgc gttttgcccc gaatgcctgc acctcaccaa
    26701 acgtgccggt atgcgccatc cacagcacac cgtcagacat tttggccggt ccccagcgtt
    26761 tcagaagctc atcactgatg attttcaggt ttgcatcatc cttatagggc atgacgacat
    26821 agttgtactg gcgatcgccc atacccgcca cgcttcgggt gatatccggg ttagtggctt
    26881 ttgttttggg atacccaagt tcaatggtca gtccacccgg tgtggtttct ccgtcatgat
    26941 aattaacacg gatatcatgt acggaacatt caccggtaaa acgggcatta attcccatac
    27001 ttcccttgag tccgctggcg ttatctgcta cctgacctgc ataagcactg aagggcgcat
    27061 cagcatcggc attaataaca gagaccagag cgtcaataag ctccttaccc ttctgccctt
    27121 tcgtgacagc cagcagataa cgccgaccgc cgatataaat gtgcagaaca ccatcctgag
    27181 aggcggtgcc aattatattc atgagacatg cctgagcctg accggtgccg ctcccctgag
    27241 caatggcata cagttcagca tcaggattga tggcgataaa ttccgccacc atcagggcaa
    27301 tcatggagcc gcgcccccag aattctgttg cctgagacgc acgggtgatc ctgaccggaa
    27361 catccagaac accggcaccg tccacggcat tctctttcat tctcgcctga ccaaacagca
    27421 gcacacactg acgctgtgcc ggagtgccgg tgacagccat ggagttatta aactcaacct
    27481 caatcagcgg tatacggcgg tcattaccga tcccgtcaaa attaatcatt gcggtttctc
    27541 cttatcggat gccggttggg cgaccggttt ttctgtcgca gtcgttgcat ccgcagatac
    27601 agccggagcc tcttcagcgg atttttcagc ctgaatgaca tcgccgtcat caagacgccg
    27661 acaccagaac ggcgtaaagg gcttttcctc cccctcctgt gccagcaggc gcattgtgtc
    27721 gggatcacga atcaggcgtc ccggcgcggg tttgataaag atggttttca tttacagttc
    27781 ctccggcggt tcgaccgccg ttgtcgtgtt ccccggcaaa tcaatatgtg cctcaaacgg
    27841 cggcgtaccg ggcggctcca caaaggtttc gtaatgacgc aggaagtcgt ccagcgagct
    27901 gatatcggtc agcggtgcga tcatttcctc gcaggagaaa taaagcgcat acatcaccgc
    27961 accactgtcc gcctgggttt cggtgtaacc gttgaccgcc ttttcaaagt aaagcggtga
    28021 ggttttttct gttctgaagc cgttaagtac ggcgatcagc cgggccacaa tctgatacag
    28081 tccgggacgg ctggcctcac gcccgttgag catgtcgccg atgacgtaga acacccagtg
    28141 actgaccagg cgaccacggg tacggccttc accggcaccc agccaggcaa cgtagatcgc
    28201 cggggcgtta atcagcatgg tgcgcagtac gctgtcgctc cagtcgccgg gatgcgtgtc
    28261 aacagacaca agttcattcc cgaaatactc acggatacgg gcgatgtatg cctgttcggt
    28321 ttccgtaatc atatgaagcc cttctggttg cgcccgaata ccgctgcatc agactgaacc
    28381 tgtggtaaat ccccggattc aggggccgca ccgttcgtat ccacaccaac cggcacattg
    28441 ccgttcatga catctttcag ccaggccagc gcttcacggt aacggtcacg cgcctgatcg
    28501 gaggctcgct gatcgcacag gtaataaaag gcaatcgcgc aacagtgctg aacaagaacg
    28561 gccggaataa cctccagcgg cagcgtgtaa cgggcggaca gatagctgtc gataagggct
    28621 gacgcatctg tcagcgcccg gttcagcttg cgcgtgtccg gctcatccga acggggcaca
    28681 gccagtaacg gcctgagcaa atcctcgcgg taacgcgccc gcatatcggt ttcagtggcg
    28741 taattcatgc gcgggccttt ttcgcctgtt tcgctttgtt accggtcttt ttccggggag
    28801 tatcctgtcc ggcttcggct aaatctgtac cggccactgc atgaaccaca cccgccacgc
    28861 tccccgcatc atcagcaggc gtctgtagta ttcgcacagt gaggcatggc tccgcctcca
    28921 gtcgggcaag ctgctccggg gagacctcaa cctgctgacg tccacgcgtg aacaggaacc
    28981 ccgcacgacg aaacgccgaa cgactgcaac gcacttctgc cagcactgtg acagaattgt
    29041 caccactgac ctgaaggcct gaatcatctg ttgctgccac agtgtgatgt tcattcatat
    29101 ttgccctccg caggggccgg gccgtaagcc cggctcacca tttacagata atccgcgaca
    29161 accagctcca gcttgccctt catctcgtta gaaacggtgg cgtttccgtc tgcgaacagt
    29221 tcacgctcca gcagctgcac cgcctgtttt tccagcgagg tggggacaac aatatgggtg
    29281 ggtttgatgc cgagtttgcg gccaccgtca gcggtaaatt cgcgcatggc tgaccagccg
    29341 tgccacagtg catccagcgt cagcggtgac tgcatcatgt aggccatctg ccagaagccg
    29401 tagcccacat tgcgacgggc ggatgcaccg aacacaaatt cgttatcggt gaatgcgcga
    29461 ccttcatcga ctttggtctg ggcaaccagt tcagccttac ggcgatcctg ataaatcagc
    29521 ggttttaccg cgcgggagca gtcaagcaga taccaggccg gaccgctgta atcagcctgt
    29581 gcgccgacag agccggtttt tgccacaaac atattgctga ccatctgcgc atccccggag
    29641 ccatccactt tggggtaaac cggatgttcg gtatcaaaga agttctgacc gtcatagcag
    29701 gccgcactga tcccgtcacg cagggcagca aagaccagct catccggttg taccgccgca
    29761 gcgcgcccca tctcctggaa caacggggaa taaataccga ggttgtcgtc ttcgaagtca
    29821 tcgcggctaa tggcgacagt gccttcaaaa gttttgttca cgatggcata gccataggct
    29881 ttcatcttct cgatgacgcg ggagccgatc cactcacgga actgcgggaa ctgccccagc
    29941 cagccgtagg tgttggattt cgatgtggag ggcacggtca tcgcaatttt tttatactgc
    30001 gacggggcca tggacatccc ggcctgaaaa tcagaacggt agcccgtcat cagggcggtg
    30061 atcatcgccg gtgtaatcgg ggtaggcatt attgcatttc ctctttcatt ttcaggaact
    30121 cggcttcggt tttacccagc agtttggcgg ccgcgatatc ttcagccgac agcgcagcgg
    30181 tggcagtctt tttatccggc acggtcacgg tgtcggtctg aagagaggtc agggccgcaa
    30241 ccggctggcg ggcgtcaagc tgtgcagaaa gtgccgcaac gccaatctgc ccgcccagtt
    30301 gttccagata gccgcgttcg cttttgaaga tgcgcccttc ggattcagcc ttgtccagca
    30361 cctgctccag cgtggtgctg ccgtgctgcg ccgacagcgc aacgtattcg gtacgcaggg
    30421 cgttatacgt ttcaacgggc acgtatttcg tcagatcaat actgccgcca gccggtgtgc
    30481 cttttgcggt ttccagctct gccgacagac tggcgacctg tgttttcagg ttgtcgtgtg
    30541 catccgcacg ggttttgatc ccggtgaggg cagacagcgc cgccgtgccc agctccggcg
    30601 taaattcgtc accgtcagcc acggtcagac cgagcgccgt cagcaactgg cgtaattgct
    30661 cattcatgga ggtatccttt aaaggagggt ttaaggcgtt ataaaggtca tccgccgaca
    30721 gcgcggcgac aggattcatg ccggtcagac cggggtcacc ggtaatagcc agcatccgaa
    30781 gttcgacagg ttcgccggtg ttctcgtcat agccaatcac cggtgaaaac caggggaatt
    30841 cgttattacg cagatgttca agggccggag ggttccactt cgggcgcacc tcgaagccac
    30901 gctcttcact gaagcgaaaa ttctccggtg aggccatcac aaaaccggca gccggtgcct
    30961 gatgcccctt aatcagggtc tggtggttgt agtcaatttt caccggctga ttgagcgcca
    31021 caacccggga gaccatgcgc tcaacggctg catggttgat cagccagccc tcggccggtt
    31081 tttccgggcg accgtcacgg gcctttaccc gaccggctgg catgatctga caccagtcac
    31141 cgtccccgtc tgcggacagg ctgatggcat tcagaatggc ataagcaagt ttgggcgtgt
    31201 ttttcgtctt cattccggca gcataagccg ggaattttca gggcgggttt tgcgggattt
    31261 caggaagtgc ttccagggga aaaatacaaa ggcgcttcac gcagatttta aagcccgtta
    31321 aatacaggtt agaaaatcac gacacgcgcc tgaagggggt aaggtaatgc atttgcatac
    31381 cttaaacaat taaggcgttt ctgtggcctt tttaaagcgt tttctgattt cgtccatgat
    31441 ctcctgctct gccacctgat caaagcccat atacggacgt gcaccaatgg ccgccggtcc
    31501 cggcggcata cctggcaggc caccccactg atgaatggcc gcataaggct catttgatcc
    31561 aatcagcgcc caggtgtcgc cataatccgt ggtcaggcgt cgggccagat cgccgttcag
    31621 cgtcaggatt ttgccgggca tgtatccctt acgggtacgc cacttgcggt aaggatctga
    31681 ccagtcatgc cagcgttcgc cgtccggctc tttttcctgt tcaaacgcca tttctgacga
    31741 tgacagaaga ctcgccgcca cactgcgggc caggtctttt ccaccaccca caaactgaag
    31801 ccgggcaaag acccgctgga gacgcgcaac gtccaccacc acggctgcat cgatggatga
    31861 catgttgcct ccttaaaact atctgattaa aataaataca ggccggtgta ctcttaactg
    31921 gtaaagcggc ataaaccctc aggggtcata tgtttatgcg ggttcgaccc ccgtcaccgg
    31981 cttacaattt accttccagt agttccagct ttccgccctc tattgctttt tcaatatcaa
    32041 atttatttac ccggtaagca ttaattacca catccagctt atccggctgg cgcttcacac
    32101 cataaggcgc attgacggca atctttgccg ttccgtcacg ggtttcagtg atgtaaagca
    32161 ggttctgatg gacatgatcc cacagcacgg catcgggggc agccagcatg gcgggaagct
    32221 gcgcgaaatc ctccggcgtc agggcgatac cgtcattctg atgtttcacg ctgtccgcat
    32281 ggagcacatt tttaccgctc atcgccagca ggcgggccgg tggcgttccc gtccggcttt
    32341 ccaccgcctg cgcaatactt tcggtcataa agcccagcgt gcggatatcg ttaccgcctc
    32401 gccgggtttt catgatgttt tttgcccaga tgcggaacgc cagctgccgc tccgggctgt
    32461 tgttcatctc ctgcaccacc atttcccgta acgccgggct tttcacctcg atcagtttac
    32521 ggatcagcgc ctggtctgtg ccgaacgccg ccgaaccggg gttatatgac cagcccacat
    32581 ccggtgtcat ggttctggtg ccatctgaat atgtggtcac cggcatttct ctgacttctc
    32641 cggtctgctt atccacgcca gcctcaacat tgcgggtgga gagatgatcc tgaccggatg
    32701 aaacagacag ccccatcgca tccagacggg cctgagacaa tggccggaca cggcagcgac
    32761 agttccagcc attgggtggg tagtgtgtat tccagaacgg atcgtcatag cggaatacct
    32821 gcccgttaag ggcggaatgc gccgggcggg tgcggctgtc catcaccgcc acatactgcc
    32881 agaacggatg cgtgtccgtg ttgttcatca tctgcgtgta acgcccggca ttgtaagcca
    32941 cgcgggtgtt cacgttgtaa atcagcgcca gacggcgggg actgcccagt tgcacttctt
    33001 ctgcgttacc ggcgctgtcc accacaatct gctttcccca ccatcccagc ttttgcaggc
    33061 ggggtgtcag tgtgcggata aatgctttct gtgaaatccc ctcatcaaca gcacgctgca
    33121 cttccgcctg tagtgtggtc agtacgtcca ggcgtgccac ttttgccgcc gtgaatgaac
    33181 gggcatgaac atccgcgtct gtttcgaacc agttccagct gatatgcgcc cctttggcgc
    33241 ggaagtatgc caccgcctct tttgagggaa gcgtggcggc ataacctaaa tcaatcccct
    33301 gtgccatcca gcatcccctt catttccgca gcaaacatgg catcactgag cagcgtcatc
    33361 aggcgggaat catccatttc acggtaaagg gcaggcaggt ctgccagtgc ctccgccagc
    33421 ccccgcgttc tgatggcttc aatgaccggc tccagtaccg ggtcgatggc ctcctgtaac
    33481 cggcgggcag gcaccgcatc ccccatgtcg tccagttcat ctcgggggct gctcttcgct
    33541 tccggcagtc tggcagccag cgctgtctgc tctgtcttct cctgtttttc cggtggcatc
    33601 tctttgtctg tctgagcagg ttcattgcca ctctgacgga cacggaagat ggcttcaccg
    33661 ggggcaggct gtggaatacc ggtctgctcc cgtacccagg gatcaggaat atccatcccc
    33721 gtactgagct gcatcaccgc actggtgatt ttggtgatat cccccggctc ttttgtctgg
    33781 aaacagatac gcggcaggcg acggatatca atggcgtggg cggtgttcag ggcatacagc
    33841 ggatacacca gatcgcggtt cagcgtggcg gccagctgac gtaaatcaga atccctgatt
    33901 tcccggcgca cctcgttatg cacttcaccc agcgaacgtg cgcccttgtc tccggcttcc
    33961 gtggtcagcg tgccgccgag gatagctttg gagatggaac gctcccccca cgaaatcatg
    34021 gtttcaaacg gatcggcctg accgttcgct gccgcctgaa actccagcga catcccggcc
    34081 gggatgatcc cgcctgtacg tcgcccgata tccatcaccg cccgcatcag ggcgcttttc
    34141 tgctccggtg ttgccccgga cgggtattta ccgaccttca tcggcaggcc gtacacctcc
    34201 agaaattcag ccagatcgcg cacggaatag tttttgaaaa tgaacggcca gataagcgtt
    34261 ctgacaagcc ccgtcgcacc accgtagcca gtgcgtgaac gcgactgatg cactatccag
    34321 ccaaagggct gaaacgccac cccggcatgg ctgccgtcac gcagccgcag ttcgctcaaa
    34381 tcatccgggt taaggcagaa atgcccgctg tcacgccagc ggatggcgcg gataatgtgc
    34441 attttaccga gcatcccgtg ctcaatctcc atacaggaat agcccttcag gatggcatcg
    34501 gtggcatcaa acagcatggc atcaaaccag tcggcggaat gcagatattc gtcgagcatt
    34561 tccgcgtcct ttttctcatt cgcgctggcg ttcgggggcg gctcaatact ccatggcacc
    34621 ccctgaatgg caaggcgtcg cttgcccagc tctgcaaaaa ggtgggtatc cttttcttca
    34681 atgtcagcgg ccagatcgga ctgggcaatc agatcgccac gttcagctcc gcgaaggcac
    34741 tgtgccgccc ggttcggggt gatacccgag gccggatgtt caatataacg gctggcaatc
    34801 tgcggaatat ccagcacggc actctgcatc tccgggtcaa aggagaaagg ttttccgtca
    34861 agatcaatta tgcgtcccac taccagcacc cccgatcaaa ttcatgatat gcctcatcgt
    34921 catcacgata accgccctct atggcccgcg accgctccgg cagcgcctgg caggctgatt
    34981 catccaggat aaagccctcc atgtatgacg cccggtttgc catacagagc gccacggcaa
    35041 aatcaccatg gcggcgggcg ttcacagccg ttgcgttctg gtccttcgta cgccccttgt
    35101 cgatttgcgg gataccgttc accaccttca catggcgcaa atcatcaagc gtggtctgat
    35161 ggcgggcgac gaggatattc tgatcctcaa actcggcctt cagttttggc atccactcgc
    35221 cataccactt cggcgataac atcacgcagt cgatgatgtc cgggccaaag gccagcagcg
    35281 cggcttcggc cagatagccg ccgttaccgg tggcgtcgaa cgccgcaccg accagcgccg
    35341 gaacgcgggt caggatgtac atcatcacct gctcctgctg ggcatacggc aggttgcgca
    35401 gctccacgcg aaacacctcg cgttttgcca gggattcagt gatttccagc agcacaaagc
    35461 aggacaggtc tccggtgcgg gcaaagtctt ccccgaagct gaagcgggaa cggggattta
    35521 acgcctctaa aagcggtttt aaatgttctt cacaccaggt taaaacctcc gattcacgca
    35581 gccatgcggc acggctgatg aagtcgtccg gggcttcaaa cgtcagaatc gggatgtcgc
    35641 ggagcatcgc catttcaata agcgcgtgag gaatataggc accgccggat tttttcggga
    35701 tacagccgta ttcctcgtcg gcatcctcac gggtcggggc gtttttgtag agatcatcac
    35761 gccacttctg ctcgctttcc ggtgaccatt cgcggccggt gacataacag atacgacggt
    35821 acagcccgtc cgcaatggca tcatccaggg taatgcggtg gacgctgtaa tccttgcgcc
    35881 cctcgcgggc ttcctgaatg tactgattaa acagattatc gacgccgtta tgcgtggaga
    35941 taatacgcac gcgcgcgccc cacatggtga gcgccattgc cgccttgaga agctcatcca
    36001 gtgactcgtg gaacgcggct tcatcaatca ccacatcccc ctgaaggccg cgcaggtttg
    36061 acggacggga agacagtgcc tgaattttga atccactgtt cggaaagcgg atcatgtagg
    36121 tcagaatttc ttctttttta tcccgatccc agaaggtctg ctcatacaca tcagcatctg
    36181 ccagctggtt aaaggcacgg gagaacagtg cgcaggcaga aatatattcc agcgccatct
    36241 cctgctttga ccccacataa aacacattgc ggccaccacg ccgcttaggc tttgcggcgg
    36301 taatgacgtt acgcccggct tccgcccagg taagcccggt gcggcgggat ttttccgcga
    36361 tgcagacctc gctttcatcc tcaaaccagc gggcctgata atccagaaat accggcatat
    36421 caccgggcaa atcaaggctg tccggcacat ccacgcccag cagggctttt tcaccggcca
    36481 gatcaatttt gcgggtggag acggtattcg tcagtaaggg ggagagttct tcggcgttat
    36541 tcatcaggct ttacccaaca gaatcccctt aatacgggac tcaagctgtt cactcatccc
    36601 gtccacgccg cgcagctcgt cggtgacggc gtttgccatc tcttcagcaa atgcggcccg
    36661 gatttctttt tcacgcttaa tgctgcgctc ggcggcactt tccgcacgct gtgctgacag
    36721 gaggatgtcc ttaataagac gcatatccac gtcgctgtca ctgttcaggg attcggtcgc
    36781 ggcacgcaga cggcggtaca tcagggcgcg ggacatctcc agaatcagcg ctgtggtttc
    36841 accggttggc ttgtctccca gttcggccat catggctttt gtctgctcgc gtaaatcacg
    36901 caggttacgg gcaatcagct cattacggga ggcttcccgg ctgatggccg ccggtgaaag
    36961 ctgctgctct tccggcaggc ccgcctcacg gatcagacgg ttgatttcct cgcgtatctg
    37021 gacctgcgtc agacgttttt cacgcagcat ttccagcagt ggcttacgga tgctgtcggg
    37081 gagcaaatcc accttgcgca cgcgtccacg cgtcggcttg tccatcgtta cccccttgcg
    37141 cgtggtttct tcacacccgg aacgctggcg cgaccttccg ccacatcctg accccggccg
    37201 gtcagttcgg cgatgaaata accgttaacg agcgtgcgct tacgcaccag tccctgctcg
    37261 gcaagccagg caatgtgggt gtgaacggtg tcgcgggaca cgcggtggcc gtaatcatcc
    37321 agacagtcct ggagcacgga ttcacccagt tcaccgttgt aatctgccag cgaccgcaga
    37381 atgaccagac gctgatcttc agtaatgaaa tcactcattg cgtttttttc cttacagcct
    37441 gctccagcag cagttcgttc tgataggaga cagagcggag cgtggcgttc gtggttttca
    37501 gttcaccccg cagggtcacg atttccacat tcagacggtt gacctcggct tctgtcggca
    37561 gtgacgacag gcgggtttcc accttctcaa cgcggtcgga gagtttttca aaggcctcgc
    37621 gggggacaaa cgttttgcgc atcagcgcca tgaatatccc gccagcggtt gccgtcgccg
    37681 aaaggatcgg cacaacataa tctttaacga tgctgaccca catgacgggc ctccatgatg
    37741 ttctggcagt tcacacaacg gatggcatcc ggcacagcaa caagacgtgc ggcggggatc
    37801 tctccgccac agtccgcgca gacacgtctg cccggagcat ccattgcccg gcgcttacgt
    37861 gtcagccggt tacaaagggc gcgttctgat ccccgctcca tcacagcctg agcacgatct
    37921 gaatcatcca tacctttttc cttacttcgt ctttcctgcc ggaacagcag gcgttccggc
    37981 catatcagta aattcatgtt gtcgccgcgc ctccagacga cgaatactgg ccttgtccac
    38041 gttgcagttc ttgatcaccg ccagcagttg caggttgtaa gtgaccgatg ccccaaacgt
    38101 gaacggctcc ggcgcaggag ggaccaggca gtcagccagc cattcagcgg ggatcggcac
    38161 cggcttcaca ggcacgtatt tcacagacgg cccggcgcac ccgatcagcc acatcatcag
    38221 gaacaggggc agaggccgca ggcactgccg ccagcgaccg gcggacaacg ctctgctgcc
    38281 cctcgctgtg ctgaatgttc tgttgttttt cattcgtggc cgcctcactg atttgtgaaa
    38341 tgagttgcag ggtgtgctgc tgatttgcca gcacctgacg cgcctcatcc cgctcacgca
    38401 caagcacctg attattgtct tcaaggcgct gcgtcttctg gtgcattccc catatagcgg
    38461 caaccagtac agcgataatc cccagcgccg ccatgcacgc cccggcaatc agggccggta
    38521 aggcattatt caccgcagcc acaggacacc ccgtaacagc agtaccgcac acagcagcgc
    38581 tatcatgagc ggtcgccaga agcggttcag actttcggca agtcgtagcg acataaccat
    38641 tcatccgccg aacggcgggc ctcaaggccg gggagtttca ccccctttga ataaatccag
    38701 cggatatact gcttacagga cgcgggcatc tgcccggcat tgatcaggcg cagaagcgtg
    38761 gagttgcgaa agttcgtttc accggcccag aagatccacg aagccagcgc caccgtctga
    38821 ccacgggtaa gcggcacctg aacacgccga tcaatggccg caaaggccca tttcatgtcc
    38881 ttttccagta gttccaggca ttcttcatct gtcagggtca tacccggttt cacatccggg
    38941 ccggtatggc cgtaacagat ggtgggtgta ccggtgggat cgatatacgt ggagttttct
    39001 ttcccttccc agtaaccggt gtaatgcgtg gcaatcgtga acgtcccggc accggccaga
    39061 acaagggcaa tcagcttttt acgcagtggt gcggggagtt ttggcattgt tacgctgtct
    39121 catgatgttc tctgagtcag cgtaacggtg aaggagaggc ggcaggcttt gcggaatatc
    39181 agaaaagtgg cggttgcgtt cttgccagat gcagacggcg ctgttcgcgt atgacggtat
    39241 aaatctgggt ttctgacatc cggtaatggc ggcgaaggga ttcaattttc tcgccccggt
    39301 cccagcgcga aaatatctca ttgtttcgca gctcggcaaa gagagattcc ccgaccggca
    39361 ggtaataacc ccgtccgccc atataggctg cctgagccgc agccaccttg cgtgccagtt
    39421 taccggccag taccggctca atcccctgac gctgaagttc tgcgccggtg acttcaacca
    39481 gttcagacag ggtccgcggc cagttctttt tgagaacatc atcagggatg tcatcaaggc
    39541 ggtcgataag ggcgtgcagt tgctcactgt caccaccaaa catgctcatc tgagtttctg
    39601 ccatatcagc ctccggttct cgcgttcccg gtaagtttaa aataaaaatc ccgcgttgtg
    39661 gcgggatttg gagttaacgg ggaagtgatt tttcatactg cctgcaaacg gcgtcatacc
    39721 ccatcagcgt gcgttcgggc attcccatcg cggccagcat tttgcggcgg tgccatcgct
    39781 tgagacgctc cagcacatct gaggccaggg cggggtgttg ctccagccac tgccagtttg
    39841 ccacaccttc gccgccgttc tgcgcggccg tctgcgattt cacccagcgg ttaagggcgg
    39901 tttccgcacc atcggtgata aagccctgcc ggtgcatcac cttccagatg gcgcgaattt
    39961 tggcggtcac cgtacccggt tttaaggccc ggttaaccgg gttctgacgc actttaaaac
    40021 cgcgtttttt gaaaacatcc agcacgcggg ataactcatc cggtgacata tcccggcagc
    40081 tggttttgcc ggtgaccgcc agtagtacgg atgtgtaggt ttcatcatcc agggcaagct
    40141 cgcgttttgc cacatgaatg gcagatatca gtttccctct catgctgtat caacactcct
    40201 gcggatatga taggtattat tttcccgatc gagctgaacc cgatactccg gaggaatcac
    40261 aaaaatatta aaaccatcat cagtttttat tcttgagtag cattccccct tatgatatct
    40321 ggccagtgca ttaatcagag aagtcgcagc ctgcccaatc aggtattttt tatgaaaaat
    40381 actgataact ttccggcaca gccatcgtgt aatgacgaat gcaccgaaaa aggatgtgat
    40441 atataaatac cagaacaact gcgaactact gtccattatc tttctcctta ttcagtcttc
    40501 tgcggcattc ggcacagtca tcagggcttt caaatgtatc cggctcgcga ttatgagcca
    40561 gatataacca ccgtccacat atactgatat tttcgcctgt ttaaaaaaaa tgatgcttcc
    40621 tggccattag tggccgcgcc catccagcgg gtagactatt catttgcttt atcctccatg
    40681 ctggacagaa tcatatgtat taatgtcggg cgtggagata tacgcatttc attaagcgtc
    40741 tgaagggcag caacagctgc ctgtttatcg gtgcctttgg caatttcagc aagagaatgc
    40801 acaatccata atgggcttaa cgctacggct gaatgcagaa tcatgtcatc cataataatt
    40861 tctggcatat cccgtttgtc acattcttta tccataaaag aaacgatttt ttctgcatct
    40921 tccggtttca tgcctccggc gatgagaatt ttacgatatt cataagacga tcttaattca
    40981 atactcatca ttcacctgcc tgtttaatca gtttccagtg ctttgctacg ggcaacagcc
    41041 tcacactcaa aaaataccga ctccggcaca cgctccttta attcacgaac aagaaaatca
    41101 ttaacgagtt catgacgtct tatatttctt tccttttcct cctggcgcaa tacagcaagg
    41161 cggtattcaa cagtccggcg ttgtttctga aagacagcca gtgcttttct ggcagagtta
    41221 cgccatgtat catcgccaca gggttcgtat tcaagctgaa atttaatctc ttcaatggtt
    41281 tcttttattt ttgccagcaa gctaagacag tcgctgattg tcttcagttt ttcattatca
    41341 atataacttc tcacgcattc acctcccgtc attccggtaa agctgaaata tggcaccagt
    41401 gcgtcacctg tccgtcctca tcttcaaggc aggctccggt ttcttcatca ataaaaaagc
    41461 cttcataccc catatggccg atgcgcagtt cctgttggtc accatactga acaatcagca
    41521 cgacagcctc catatcaggc gggaaagcat cattcagtga atgccaggga taaccgcgca
    41581 aatccccgaa ataaacaccg gtcagcgttc cgccacagac cggcattccg acggcacaca
    41641 caaggggttc atctgattca acaaataatg tcgttatcat ggcattaatt cccgtaatac
    41701 aacatcttcg gcagtccgac gacggtgata aataaaactc accgtgccct cggataattc
    41761 aaatttctcg ccgatttccc taaaggtaag acgccgtggt gattcagcat cccgtaattc
    41821 acgaataaga cgaacatcat cgtcaggtat gcgggtgaag ggcagcaact ctccgtattt
    41881 tttcatactt acgccaatat atcgcgcccg attgtggaca gaatcccggt ctcgccccag
    41941 atattctcct atttgcttac cggtcattgt tctggcattt tttcggataa atttatcttc
    42001 atcaggttta aatctgaggc gtttgcattt gagcaactcc gggtaagtac cgcgtaataa
    42061 aacaatccgg ttatgtacac cccagaaact tctttttatc ctgacagcaa tatccttaac
    42121 cggggtggat ggataaagcg caatcagcag cgcatcttct tcaggtgtcc atgcgcgaac
    42181 atgagcgggt gcacggcctt ttctccccat gggttgtaat atcatcatgc atcaccccct
    42241 gactcgcttt tcagcagcat aaaactcatc agacagaatt tcggtcattt tctgttttgc
    42301 gggttttacg ccgcactcaa ggtaaatcac atcgtcaacg cagaaccact ccacagggcc
    42361 aaacaggatt gccgagaaat caatgcccag ccagaacaac agtgcatcgg ttctggcata
    42421 cgtgacgggc gaatattcac gccacagact gttaagttcg tcagaggcaa cgcgcagggc
    42481 tttgggaata cgtgatgtgc gcggtgtgca gctccagcca ttcgacgcag tcggttttcg
    42541 ccataagtcg cgatgaaaag gatatttgtc atccataaag cgcagccctt taaaacaaaa
    42601 accactgata ctgaattcaa ataccgaccg gcacgaaaca ttcagcacgg cctcgaggcg
    42661 ttttgcctca tccttaactt tctggcagtc ctgctggtat ttttgccacg cagccagcgc
    42721 ggatgggttt gatgttttaa agaacattac gccacctccg cagaaatcgt ggccggtgcc
    42781 gctgtccggt caacaatcag gtaacgcaga acatattcag tgatattcca gccgcttaaa
    42841 ccgcagatga tgacgcccag gcggatgtcg atatacgcca tcacaaactg gggcacgcct
    42901 tctttcatgg cctgctcatc aacttcagcc accacataaa cggtttcaca ggtcagaacg
    42961 ccctgagcaa attcccacgc catcgacgga atatcatgcc cttcaatcca cggcagggat
    43021 tttctgaagc tgcaccactg aacatcatca gccgcagact cctggctgca tttgcgacga
    43081 accggctgca ccgggcgggg acgcggaata tcataaaaac cgttacattc cgtcagcacc
    43141 ccggcatcaa ccgcatcacg cagaaaataa accatggagg atggcggcat attcattttt
    43201 tcagccagaa cgccgcaggt cagacgcccg taaatactca gccagttttt aaccccttca
    43261 agcacttttg catcaatcat gaattattcc tccgtcagtg ccacatattc agaatgttta
    43321 atctcctggc attcttcagg aatacacccg tttatttccc tgccataagc gcccacttta
    43381 aacaccacgc acagggcatc acgggagaac gaaggggtcc accaggagtc cgtgttaaac
    43441 atggagggga ttctcattac ccccagcttt tcacagagcc agacaaggaa atcaggatgc
    43501 tttcttaaat cgcgttcagc ggcttcaacc attgcggcaa tcgccggtcc ttcagggcat
    43561 aactcatcag gtctggcaat aaagcacggt ccgtccccga taaaagccac atcatcgcgc
    43621 ttacgccagc ccgcaggcag agcaccggaa aaataaaact cactgattct gtccatggcg
    43681 aacggcttta agcgcaatcc ggcagcgcct gtatttttca gtaatgcccg aagaatactg
    43741 ttgcgtagcg gtaaaacagt ctgttgatat ttggtgaaat atttatttgt ctgcacacgg
    43801 tctaatttga aataaaaata tgccatattt cctgttcctt attttcagtt tacaggcgca
    43861 ggcagtcccc tgacgcgagc gccataatta aaacgatgtg atatttaaag atttaatgcg
    43921 gtgttattta ttcagtccgg cgtcctgttc aaaaggctcg acataaaaac tttcagcgcc
    43981 tttattcacc ttaataccgg caatcccttt caccgcatcc ggctccgcca ggaccgcttc
    44041 cttgttcact tcctctttcg tgcggataaa acgctcaagg cccatacggc gcagcatttc
    44101 aatcacacct tccacatcac gactgacgct gcatgatgga ttccccagtc gccatgacac
    44161 cgttccggtg gtcagattgg cagttttggt tttgccgccg ttcgtcagct catcacggtt
    44221 ggttttgcac cagtcctgaa tccccttaaa aagcactttg atttcttttt taaggttttc
    44281 aatctgcggc gtataacggg cggtgatttc tgccacttca tcattcatcg ctgtttccag
    44341 gcgcagcgcc tctcgctgaa tatcgcccag ggtgcggata tcgcggctga cctcttcacg
    44401 ggtctgcggt gccgcctctg ctgcggcctt taattttgta acgcgttttg ccattttgtt
    44461 tttccttatt gcagtgtgcc tgatgcttta tgcacgcggt ggcgtttaat gatggcatcc
    44521 gccccaaaag cctgtacaga tgcctgaaat tccgctgaga gaaactgaat aatttcagga
    44581 ctgtgatact ggagaatact tgcataaacg tcacagagtg acgtcggcct gttattcgta
    44641 tcacgttctg tttttacatc cacaccaatc ttttcatgca cttcaacaca gtcattgcct
    44701 gccggttttt cgtcatgcga aacatgctta aattcaaaaa taacgcgtac tttgctcatg
    44761 gaattacccc tgctgattaa attcattacc tttaataacc ccggaacgca tgctcatatc
    44821 tttcatgagc gcactggctt tcattgccac aagttcagca aagtctctgc tttccgcgag
    44881 cataaccacc ccaaaaatca tggcgggtcc atcacctttc tgctttatcc cggttgaacg
    44941 tatgtccaga cttaccccca tgcgcactgt tccatcagcg tttttatgga taacgtgctc
    45001 cttgtattcg aaaataatgc gtacgacttt actcatcctc tggtcctcgt tctgtttgga
    45061 taactgaccc ggcgtctgac ctcacggcag aaccgaatca tggcattaaa ggcttcttcc
    45121 tccgtcaccg cttccggtat ccccggaacc agtaactgct cctgttcatc acgtccatga
    45181 cgcgccataa cctcaatcac atgccgtacc gtggcaggct ttccggcaac tatcggcaat
    45241 gcaccttccg gcaggacgta accaaactca ataagaccac tcgaccacgc ccatgcaatg
    45301 agattttttt tcacctcaca cctcccagta aaccgtgcag ccgctgatgc gggtggcctt
    45361 cacacggcgg cgcaggccgt tggtctgtat ggtgatttca atttcaccgg ttccctgcac
    45421 gttgcctacc ggcggcgtgg ttttcagtgc aaaacgttgc ggatagcgct tactgcgttc
    45481 cagtaccgca ccggcaagtt cggtcagacg gcgcgcgctg ttcagggagt cgaataaatt
    45541 cattttgtta ccgcaggatt gcatattcat ataacacctc atttagctga cttgtttcgc
    45601 accaacagaa cggcatattc cgctgtaaac gtctgtcgca ccttctcgat caagtccggc
    45661 agatatcacc attcgtgtgc ggtcattaaa ttcaaaaagt aaatcgccac attctttatt
    45721 ttcggccagt gaaatcacct tcacgttatc gaaattaacc aggtaaaagc gtccgtaaat
    45781 atcaggaata ttaaataccg ccataatcac acctgtgaga gtaattcagg gttggtataa
    45841 acctctttaa aggccgcatt aatatgtttt tctgtcagcg ccgcgccttc accgctggcg
    45901 gtgagccacg cctggttaag cgtatgtgtc agaacgcgta acgctcccgg cttttcagcg
    45961 atagcctgca tgacggccag ctcggcctca ccactgatcc cccacgccct ggcaatggcc
    46021 agcacatccg cctttttggc cttgcgaagt tgttttgtac gggcaagacg gctgaacagg
    46081 cgcgataaat catcaaaggt gcggcgtcca cctttaaaca atccgcgcgg gttaccaata
    46141 agcaccatcc cgatccccgt ggcgtcctga attgcccgga gttgctccag accgtcaata
    46201 ccaagatgat ccgcctcatc cacaatcacc agtccacgcg ttcccatcag gcgacggcgg
    46261 atagcgcggg ataatgcccc tttgttcgcg cgggtgtaat caatccccag cgcatcggcc
    46321 agctccagca gacactccgt gacgctggag tgcgcgggtg acagggtgat catccaggtg
    46381 tttggttgct cctggcagta attacgggcg gtggccgttt tacccacccc gggcacgccc
    46441 actatgacgt taatacagcc catcagacga accgcctgaa acagtgcgcg cagctcctgg
    46501 actgtctgcg tttccacaaa ctgcggcggt tccggcagtg cgctttgttt attccagttc
    46561 tcataccagg aacgcaggga agcagccaca gcggcgttat cgcctttata ttttccctta
    46621 cggaaagccg ataatgtgcc gtcggaaatt cccgcctctc tggcgatggc atactgcgtc
    46681 agtacgccgc catcaataag ttcatcaatg gtcttgatta catcgttaat atcagtcata
    46741 ttattaacct ctcatttgat accttgttta atcaaataac ctgagtcgcc gctcgggtta
    46801 tttttttatt tcaggccagc gggtcatttt cttttaattt cgcttcgagc aactgcaatc
    46861 cccgctggaa attacgctcg tattcttcat caggctcatc atcaacggca ggttgctgaa
    46921 cggccaccgt attacctacc gggcgataga tgttttccag ccagggctcc tgcggcttgt
    46981 gctccagcac gttgaccacc tcatcctcgg catcacggat tttttcctct gcgcgtttac
    47041 gcatgccttt aaggcgttgc tgctgtttgt agtattccgc gctgaccggg aaggcttcgc
    47101 gtttattgcc gtcccatacc gcctcgcaaa tcacgctgcc atccaggcga cgtacggtaa
    47161 ttcgttcggc atcatgaatg tcatagctga taagcacctt acggccatgc tcgtcacgca
    47221 gctcgggcgc gtagtaaata ttattcagcc agcgtatttc acagcgtctt acagggcgtt
    47281 ccaccatcgg ccggaacata tcccgcagct caacatcgga cagccattca atttccgtgt
    47341 cctcttccgc caggcgtttt tttctgaact ccgccgggct gtaatgtttc ccgttcggct
    47401 tcatgggtaa ttcatcgtgc ggccggttgt tgtaccactc aacaccgtca cgaatggcat
    47461 caatcagttc agaccaggac ggtaaatcac gcatcgctga ctgctgccgg gcgttcagcc
    47521 gtttgccctg ttgcagggca gtaaatgccg agcgtaaatc gcggttggtt ttgcgtaacg
    47581 tctcgcgatc tgcacctttc ccgaaatagg tgcggtattt acgggctatg cgcatcggta
    47641 atgtgcggtt aagccgttcg ataatgcctc gtccctgcgg attaccggca atcccggtcg
    47701 ggtgattaat ccccagtcgc ggcagtatcc ccacaacctc cttatccagg atgtcggcgg
    47761 tttccccgga gccgttatcc gagtaataca gaaacggttt gccgtgatgg cgaatgccgt
    47821 gctgtatggc accggctacg gcgaaaacat tttcagccag gtcaaggctc cagcccacca
    47881 caaagcgcgt gccaccgtcg ataacaaagg tcacttccgg tgcaaatggc cgaccgtgaa
    47941 ccgggtgtgc gcatttcagc ttcatgccgt gaccgtcacc aatccagaca taattcaccg
    48001 gcattctgga ccagtcgcgg cgcgtgaatc cctcaagctg gcggtattca ctgccggtca
    48061 cccggccttt ttgtttcacc acttccggca gtttcttcat tgcgcggcga atggtgtcat
    48121 aagacggcat gatatcgagc atataaggct catcagcgtg ccggtgctgc cattcagcga
    48181 caaaatcctc gtaagcctcg gtcattggtc ggccgtttga ctggcgatac tgcgccagaa
    48241 attcgggcag ccagttaata tcttcggctt ttatttcctg gcgcttaccc ggtgccagta
    48301 aaagcaggcg ttcagcggcg ttctgcgcct tgttaaaggc cgcaatccag cgtttcagcg
    48361 ttatttcact caacgcacgg ctgtttccct ttttggcgtt cgccgtctca accattgcca
    48421 caatgcgctc gtccagttgc gaacgtgcca ggttgtcaac gataaaccgg atagctttcg
    48481 cacagctgaa accgggttgt tgcgcgactt tcatcacttc gctgacgatc gcaattcgtg
    48541 catcagccac ctggcgctgg ttttcagtca gggcattaag gcgttcaacc atcagttgtg
    48601 gtgatccgcg atatgcctcc accgcatcaa ccacggcagg cgagcgtctg gcctttgtca
    48661 ccaccggagc cggtgattca tcagcttttt gcgtcatcag ttgaagggca taacgttcac
    48721 gcaatacttg ttgcgcgcca gaaggcaaac aatcgatatt aaattcgatt gcttttgtcc
    48781 ccttacgttt gcgtaacatg gctgcatttc ccgcagctgc ttttgtgagt cgatggcgaa
    48841 tgttatgttc catcattggt aaatcaggaa gaccaacaca ttcttttgct gttacccaca
    48901 tacatccccc atcaagccac ttctctggcg tccacctggt atctgctggg ccagattaca
    48961 gcaggttcaa cccctatttt ttcggcgatg gcggcttcgt atttacggca ctggcggtaa
    49021 aacacattgc gcattgatcc gctttttaat cctaactccc tttccagttc agggaggtct
    49081 gtgcctttgt tttcaagggc tgcatatacc gctttagcag accagtcaca cccctctcga
    49141 acaaacaaat tattcgatgc ttgtctggat accctcattg tgtgatcctc tcatgtttac
    49201 ctgaaccgat aatcaaaacc ggttatcgat ttaggtaaat cataatcgca aaataattcg
    49261 atatcaacgg ttttttgcga gcatatcgaa attaaatgcg atgtcgataa tttctttgtt
    49321 ttataggggg ttatatggat aaggaagaaa aaaccgtatc cagtgcggaa cggtttattg
    49381 atatggaagg aataaaccga ttttcagaac gcttagctat ggctatggga aacatgacaa
    49441 acgtcgaact ggcgaccctt tgtggactat ctgaagccac agtaagaaat tacaggaagg
    49501 ggaaaaactt ccctcctttg gataaactgg ccttaatagc taaagcctgt aattgttcac
    49561 tcgaatggtt ggccacaggc aagaacgaag atgaatgcgg aaaaatcgaa aacaattgcg
    49621 attcaggttc gggtattgat caggcgctta gctctctaag cccccaagag aagcaattaa
    49681 tccttacatt catcaggcga gaaggtacta acaaccttgt caggctggca gcaatgaata
    49741 catctacaat cagccaggat gcggtagaaa gcatcattga tgccttgcca ttacgtcccg
    49801 tactaaaaaa tgcaataaag ataggaatgg cgggaagtga agaaacagac aaagagattt
    49861 tgcgcgttat tgagaagtac aactcgagca ataacgccac ccaggaatca ggggcagtga
    49921 cagtaaagaa aaatgttgtt ggttaagcgc catcgctatc atgaggtggt gctgtaaata
    49981 catatagaga tcatatgact catcccaaga taatcacgct attgaatagt aattcatgat
    50041 gattatcata ctgattatag ttagatcgat tatgtgagca ttttggcagt ttgaagatgt
    50101 tttgaagatg ttttgaagaa gattttaagg gtaaattagc agtatcaaat gtgtcgccgt
    50161 tttttgatct tttgactatt tgttgagcag tcggtatcaa aaagagtttt ccgtccgaag
    50221 tatacatcac ctgttttatt tcagcatctt acaggtgatt tcacctcctt tcactaaaac
    50281 ccttgtcggt atcaaatgat tcacctggtt acaggtggca ctggtctgga taaccttcgc
    50341 ccatgcccgg aacgcaaccg ggtattgagg ctccacgcca aagttatccg cgggcatatc
    50401 cacccgctgg cggatcagga cgcgtttatt cagttcgccg gggtccggca gtatgtaggt
    50461 tgcgctggtc tgcgcctgac gaattttcat agtggtataa ggcgataagg agcaaccaac
    50521 cagttaaaac tcattggcaa ctccattttc tcaacgtctg taaccgttga gcggttttcg
    50581 tagaaatggc tgacaagtag cagaagtgcc agcttcacat catcagatat cacaagccca
    50641 tcaggatcat ccgcaggcct gtcatctgcg gttgcataca acttacggtt aaggaagttt
    50701 tccgtacgac tctgagcggc cttaccaagc agttcaagca actcatcttc atcagagaaa
    50761 tcatcatcca gacggagctg aagcttaatc tcttccattt ttaacagcat aaaacctcct
    50821 gtgcccgcca gaacgcgggc acaaaaaaac cgcattacgc ggcgtgctgt attacgtaaa
    50881 aagactaatc aaccaccaac gctacctttc cccaccagcg ctttaatggc agaggtgtct
    50941 tccaggatac agtcaaaacg atggaaggcc agaaaaccgg tctgatcata ttccgcgtaa
    51001 cgctcaacca gacgtttaag aatcatgtat cgcacacgac ggataatgaa gcgatcaaag
    51061 tcaccacaga acatgaattt tttacccgcc ccgatatcat caatttcctg atcaatgaca
    51121 tacggtacat tcaacactga agcaggtgcc acaccaacaa tatccggcaa ccataaaggg
    51181 cgtccctgac cgtcttccat ctcactgatc agtttcagcg tattatcgtt aaacgccagg
    51241 cggaatttcg gtccgcgacg atatgcagga tcaatgctgt gtttcagagc cagaatttcc
    51301 tgccacttca ccgtatttgc cgcggcagtc tgtgttgtgc cggtcacaga tgcgaccagc
    51361 cctttgggtt gtttaggcgt accagcacca gttccctgaa tcagataacg ggcttcacca
    51421 cgaccaatac gttcagcaat gcgacgggca agataagctt ccatatcgat cgcactgtcc
    51481 tgcagcaact cattagacac acgaattatt ttcgatgtca ttttgagcgc cccaaggctt
    51541 cccataccga aatcggtgtc ttcttcaccg gcttcttcat tttcgcccag cagaacacca
    51601 acttcggaag taccatcagc tgttgcccac tccatagtgc gaccgtcaga agtggtaaga
    51661 atctgcgcca cactggcgat gccaccgtag gatttcatct tctcaacaac tttcgccagg
    51721 aatgtttctg gtacggtata tccgcccttt tcatcctgag ctacgccctg ggcacgaagt
    51781 tcacgcaacg cctttcgttc ttctgatgtc agctcactgg caccgtgacg catccactta
    51841 tcaaaaacct gagctcgttt ctcatcctgt tgtggattgt tttccggatc aagattctga
    51901 cgctgctctt cctcattgct ttcaatgtac gcctgatcct gacgacgcag ttcttcttcg
    51961 cgtgcaattc gttcatcaag cgcttccagt tcggattttg ctttgttcca ctcagtgcgc
    52021 tgctcttccg tccatgcgtt atcaccaatt ttttcattca gggcgcgcat gtcagttgcg
    52081 atagtattac gtttctgttt cagttcatgc agtttcatga tgtttccttt acgcgttaag
    52141 aagggtcagg acgcgttcac gcgccatacg ttgattaatg gctttctgta gcgcgccgct
    52201 gttgcgcgcc tcctgccatg ctttcatgga gcgaacagcc gagtcagcct cctgataggc
    52261 aggatatgtc acaggactga catccagcag acgggaaaag cgggttatct cgcgaataac
    52321 aaccccgtcc tcatcctgat accactcctc gccgtcacgg gcgacacgga aagcaaaaga
    52381 tgactggtta atatctccac gttgcatcgg ggccagcacc agatcacgaa tggtctgtgt
    52441 ctccggagcc tggatgtcat agcgcaatcc gcgctcatca actgaaagat tcagcgtgcc
    52501 tgctgcacta cgcccaagaa taaaattagg atcgtggtta aacagtgcgc gtacatcatc
    52561 accaagcaca tcgtcaaaag cgccgggccg gatgatttcg cggaatgaac caaatatcag
    52621 ctcagaacga cagtcaaaca ccgatccata accgataatg tgcgcagggt tatcgtcatg
    52681 ccgctcagca cgcacctcac cgctgtaaca acggatttca cggtcattca ttggtttttc
    52741 cctcatcatt ttttgggggc ttaaaatctc ctgccgggtt agcagcattc acgcttacca
    52801 gcatctcatc cagcccttca accggattca tatcctcgaa tgcgcgggct tcattacggc
    52861 tcatccatcc atcggtaata gcgaagtgat agaattgcgc gcgctcctgc ggagttccgc
    52921 gtaaaagccc cgtcagattg aacctaacgt aatacccggc ggctaactca gcgcgggtaa
    52981 acaagcgacg gttaagctcc tgctcccagt tcgttaccca cggcatcatc gtgtagcgga
    53041 caaactgaat cgcctgcgca gaaatattgg agaaggtggc tttttcgagg tcattaatca
    53101 tgtgcgcagg aatattgaaa ataccggcaa tcattgaacg gttcagtttc atcatgtcaa
    53161 tgatctgagc gtcaactggc gacacagtca gtgccttgta atccagatcg gctggcagca
    53221 gcatggtttt gttttcctgg ctgcgtaacg cctgcgatgc cttctgccac tgatctttaa
    53281 gccagcccca gctgtcctta ttgagtccgc ttttaacgga tactatcccc gccggacggg
    53341 cattaccgct gaagaagctt tctgtgtatt tctgaccgct catccccatg cctattgttt
    53401 cggcatgttg cataatcgga ctcagcccca tcttctgatt attacccagc gcacggatgt
    53461 ggatcatatc gtcggggctg atcgcaaacg ccccatattc gttgtacaaa ccgtaggtat
    53521 atcggccacc agtattcatc agcgtcgttt cccacggcat acagcaatcc agggatatga
    53581 cttcaccgcg acgattacgt ttcacccagg tatacccatt cccccagcca aggatgtgac
    53641 gttgcttcag ttcgcgccat ttgtaactgg tttgccaggt attgggctca tcatgaacca
    53701 gataaaacgc cggatgatcg cgtgcgggct caaccttccc attgtgcctg cgcataacat
    53761 gcaacggcat ctgggcaagg ctggaagaca ggacatagat acaggaatac accgcagcca
    53821 gtttcatcgc agtttcagga ctgacataaa cgtctgtccg gaacagccca tcagtatcaa
    53881 cggcatcccc ggttatcggg gtggaaggat tctccagtga tttacttctg aacagagcat
    53941 caagcagcac gcgtccccct tctggccata gccagtgcgc ccaccagcag taaaccgccg
    54001 gacagcatca gagccggagc cataccaaac tgcaggtaaa acccgcacgt aagcaggcca
    54061 aaaccagcca gcccgataac atcagcaatt agtgatttca tagaattaag agagcatcgt
    54121 ccggatcaag agatgagagg aaatcgtcgg gttctttgag cattgcccga ccgatcgcca
    54181 taatcagcgc aaccgcacca tcgattttgt tttccgcctg ctccttgaca ggcttcacca
    54241 catcatcgtt acccggaatg gttttgccga ccacgttgcc gatacaccag gtcatgatgg
    54301 gattgccgtc atgatgaaag cgccccgatt caatcgccgc ttccagctct ttcatcggat
    54361 cggacatgtt ggtgtagttc tgaatgatgg tgatggggtt caggtcttca tcagcaaggt
    54421 catgcgacag cccggtcgcc ccgaaggggt cgatgggtga ctcactgacc gggctgattt
    54481 tgttcgccgc tttggcctcc tcgaggatgt accgataatc cacctccgca ccagcggtaa
    54541 cagtcagaac gcccatttcc acccatttct gaaagcgttc ggctgtccgg cgatcttcat
    54601 ttttctcgac gctgtacacc gtgtcatacg gtacccagaa acgcggggcc acactgtagt
    54661 aatgcgtttt accgtcaatc tcgcgggtat aaagtcgcgc catgctgttc atatccagct
    54721 tacgcgccag gtcaaaggcc agaatgcacg gctgcccctc gaactgctca agggtcagtg
    54781 atttatcctc gcagctctgc cagctcacca ggttgaaata cgccgaacgc gccgacaccc
    54841 agatattgag gtgttttgtt ttaaagacgt ttgccagacg ggcgttattt ttcgcacgct
    54901 gctgctgact taacaaaaat tcgcgataaa ccgacacgcc aatatttgga ttggcttttt
    54961 ccagcacctg cgggtcgatc cagtcgtcac cttcatcaac ggtatagatg atcccgaaca
    55021 gttcatcgtt gggtaccgaa ccgttgagca tctcgataac ttcccgccgc ttgtcgtagc
    55081 acggcccctc aatgttgtac ccggcggtgg tgatggccca catcagtggc tgacgtcgcg
    55141 cccccatccc ggtaagcatc gtggtataaa gcgcatcggt ggcgtgctcg tgatattcat
    55201 ccaccaccgc acagtggggt gatgaaccat caccagggtt accgatcagc ggttcaaacc
    55261 gcgcaccatc ctccggacgg ttcatgtttg aggcgttaac ctcaatcccg aacgcttccg
    55321 tcagcatggg tgtgcgttta cacatcagtc gtgccggacg aaagacttcc catgcctgtt
    55381 tctccgtcgt ggcaccggaa tacacttccg cgccgaactc gttatcacag gcaaaacaat
    55441 acagggcgac accggcagag attgccgatt tgccgttctt acgggggatt tcggtataca
    55501 cctcccggaa gcggcgcagc cgggagcctt tattgaccca gccaaacgcg cagcagatca
    55561 caaagagctg ccacggctcc agcgtgatgg gcatccgttt gaatgcccac tcacccttgg
    55621 tgtgcggcaa cagctgaata aatttcgcgg cccgttcagc caggtccttg tcgaagcggt
    55681 aacgaaacga cttacttttt tccgccatca ggtcatcaag atggcgctgg caggcctgaa
    55741 tcacaaactg gcaggccaca atctttccgc gcacgacatc acgggcatac tgatttgcag
    55801 catttacgtt ggggtaagat ttccggctca tgattcgata attttcagaa acgggttagt
    55861 ggctttcttc tgccccgcca ggccaatcag acgctggcgg ctgctggggt cgagtccgag
    55921 cattgccccc gtactgctca tctcggactc ctgttctttt ttggcggtca gctccggatt
    55981 tttgaccatg ccgcccattg caccggtgat ggtgttgccc tgtctggcaa tatttttcac
    56041 ggcacgtcgc cagaactcat aggccacgca ccaccgctca agcaccgcga ggtcagtcac
    56101 gcacagcagg ccctgaccgc agagttcttt ggttgtcagt tgccacatga tcgtggcgag
    56161 agggagatct tcttcagcga accactccgg tggctcaaca cctttgatgg gcgtaaaaac
    56221 aggttcatct ttgttcaggg ctcgcttgcc ggggtttccg gccagcgcct tgcgcgccgt
    56281 tggcttgggg cgacgcccgg aacgccccgc cgttccagcc atatgcggca ctcctggtta
    56341 aatttcattt ttcgcgggta taaaaaaacg atggggcggg cagtccggaa gacgtcaggt
    56401 cacagggatt tgacccgccc ctcccctctg gcagtgggaa ctggttctta cttcagccgt
    56461 tcacgggccg tcttcgcctt atgacacggc cagcacaggc tctgcagatt gctgtcggca
    56521 tcagtgccgc catgcgcttt agggatgatg tggtcaacgg ttttcgcctc gcgcaccaca
    56581 ccggcacgca aacacaactg acacaggcct ttgtcacgct ttagtacacg cccccggata
    56641 acatcccatt tcgaaccata accgcgctgg tgtcgggact ggcctggctt gtattgcttc
    56701 cagccctcgc ttttgtggct ttcgcaataa cctgacgggt cagtcgtggt atggcggcag
    56761 ccgcgaactc ggcaggcttt tggggttcgt ggtggcatat gtactccaat gaagaagcta
    56821 ccgacatagc ctcctccatt cgtcgtgaaa ctattttcat ctacccagta atgaattctt
    56881 tgaagagttg taatcaatac aactcgctaa tggagaggct tgtctcacac gtgagacaag
    56941 cttcctgttt gatttactgg acactataga aggacagaat gccttcctca ctcgaatcac
    57001 atcaattaag gaggttcaac atgtttcatt ccacaagtca tcaggctgta attatggcag
    57061 catcagtttg tgccacagac cttttccgct tcactttgag cctgattcat ttctacctga
    57121 ccggctcgcc tctatctttt taatccccgc tttatccaaa ttgcattgcc agaatgccga
    57181 caacagactg acattcaaat cctgactacc tccaatagtc tgaccgtaca cctatatagt
    57241 tttaattttc atcaatccat ttaactatcg tttaattgtt gtcacatagg attctgccgt
    57301 ttttaacaat gcaggataat aagatgaaaa aaatgttatt ttctgccgct ctggcaatgc
    57361 ttattacagg atgtgctcaa cagacgttta ctgttggaaa caaaccgaca gcagtaacac
    57421 caaaggaaac catcacccat catttcttcg tttcgggaat tggacaggag aaaactgttg
    57481 atgcagccaa aatttgtggc ggcgcagaaa atgtggttaa aacagaaacc cagcaaacat
    57541 tcgtaaatgg attgctcggt tttattactt taggcattta tactccgctg gaagcgcggg
    57601 tgtattgctc acaataattg catgagttgc ccatcgatat gggcagcgct atctgcactg
    57661 ctcattaata tacttctggg ttccttccag ttgtttttgc atagtgatca gcctctctct
    57721 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg ggggaggctg cattatccac
    57781 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
    57841 acgaccagcg gcaacatcat cacgcagagc atcattttca gctttcgcat cagctaactc
    57901 cttcgtgtat tttgcatcga gcgcagcaac atcacgctga cgcatctgca tgtcagtaat
    57961 tgtcgagttc gccagcttca gttctctggc atttttgtcg cgctgggctt tgtaggtaat
    58021 ggcgttatcg cggtaatgat taacagccca tgacaagcaa acgatgatgc agataaccag
    58081 agcggaaata atcgcggtta ctctgctcat acctcaatct ctctgaccgt tccgcctgct
    58141 tctttgaatt ttgcaatcag gctgtcagcc ttatgctcga actgaccgta accagcgccc
    58201 ggcaacgaag cccagatatt gctgcaacgg tcgatagcct gacgaatatc accacgatca
    58261 atcatcggta aagcgccacg ttctttaatc tgctgcaatg ccacagcgtc ctggcttttg
    58321 ggggagaagt ctttcaaacc aagctgttta cggtaagcat cccaccaacg tgaaagaagc
    58381 tggtaacgtc cggcggctgt tgatttgagt ttggggttta gcgtgacaag tttgcgaggg
    58441 tgatcggagt aatcagtgaa gagttcacca ccgacaataa cgtcataacc gtggttacgt
    58501 gtcggttgtc gtccgttatc cgttccttct gaccaagcca ccatatcaag gaaagcttta
    58561 cgctgggaat ttagtacctg cataaattac tccttagagc caccaaattt gttaccgatt
    58621 actcgcattg cagccccacg aatagcatcg acaccgatca gccccacccc accaccaatg
    58681 gcaacagaaa gtgatttagg ccatccgaca tactcaagag cggatgcaaa ggtcagcgtc
    58741 agagcgccac atagcaaaat ctcgagcgtt tttcgcttcc agccaccacc accgccaaaa
    58801 taggcgatgc gcaaaccagc cataacgatc gacataatca ctgcacccag cggtgtgtct
    58861 ccacgccacc agctctgaaa caactccagc cagtccggcc aggtatttgg gttatgaggc
    58921 atttcgtcat ctctcacctc gcgatatttg cgggtgctgt gttggaaata aaaaggccac
    58981 gcaacgtggc caccagaatt atttccccac cagttcactt acctctttca ccgtctgatt
    59041 aaaccgctct gactcaagtt aaacacctaa cgcccgacgc cccagcgcca ttgctgcttt
    59101 tattgtggaa ccgaatccca taaagaaatc agcaaccaga tcaccaggtc gactactggc
    59161 attgattatt tgcctgagca tatccgccgg tttctcgcac ggatgtttac ccgggtagaa
    59221 ctgaacgggc ttatgcgtcc tgacatcggt ataaggcacg gagactgata cggagaaata
    59281 gcgccggtga gatttaaact catccagcaa ttcagaagat ttgcaattca gtgaatcatg
    59341 agataccacc aactggtggt aggattgtct caactagtga gctttataca tttaagtgcg
    59401 atgcacagat aataaccacg ccaatggaat atcacacaca aaattgatag aacactaaca
    59461 ataagccaca aaataattca caatttattt ttacagtgga taagtagcaa agtagtgaat
    59521 gtgaataacg gctaactaag atgaaacaaa aagccccaca atatgtgggg ctccttcgcg
    59581 atttatagta aaataatcga ttctctagct gaaaaaatct ttaaaaatta tcagcaatac
    59641 gcaagttttt taccgtcatc cggtactagt ttgatcacta caccaggatt cttacgctct
    59701 aacatctgag tttgactcca tgtaatacca aaggtttcca ttggagatgc attgccagca
    59761 aagagttcgg cgacgacttt agctgcctca atctctatct gctcatgatg tttcataaaa
    59821 atccccttgt tcagcactaa gctgtagttg cttctttgaa gcataaaggc aacaaagttt
    59881 gttacctgaa gatgtataaa gtaaagcgta gtacattttg tgtgtgcttt gtgtggatcc
    59941 agattgcatc aactaaaaga ggttttcaat acttttgtaa tgattctgga gaaattaaga
    60001 atctaactca cactgattgg ttaggtaaaa cgctcctaag caccattgcg caaccacctc
    60061 gttcgtaatc agcaatgtac tcaaaaccac tttcagtata gtatcttatt gccccatcaa
    60121 ctggactaac taaagctatc gaatcaatag attcaccctg aaggttcaga aatcgtgcat
    60181 atgcagagaa gcaatccaaa acgataccaa gcatctgatg gtctaggtct tcatgtgcat
    60241 cattacgttt ttccatccag caaatatgca ccgcattttt cggcttacag taacacccaa
    60301 atgcaaatcc aataggctcc ccacgataat aaacaactag ttttatcggg tgttcttcca
    60361 tcatatccat aaacactcgc gactttagtc cgggatccca ccgcagttta ttgttagcct
    60421 tgaggaactc catatctttt agtaccagct cgtcggcata tacgagccct aacgacttat
    60481 gagggaagcg cccttttaaa tactctgcaa catttttaaa tactgctgtt tggaaggctt
    60541 gaaacatcat gtcgatccag acgagacgaa agtttaaatt ctacctactc gccgaatacc
    60601 atttcaatgc cacacatcta aaattccgaa aaaaaacccg cacatcagcg ggttttctac
    60661 tttttattaa agccggacac acaatgccta tcattgagaa aatattatcc attttttttg
    60721 aaaaatgcaa gcatcatgtc gccattttcg tcgaaaatca ttcatctcgt cactttcctc
    60781 aattgtgtct cagcatacgc ttcttcctgc cagcactttg taaccagttt atcaatgaca
    60841 tctgcatatc ctttgtacca ctgataatcc gtcaggtctg gtaccagctt ctggacatga
    60901 agccgcgcca gtgtggttgg taaacggcta aaccggtttc cattgcaacg cccacaaacc
    60961 ttataaacag gcgtgccatg aagcctggtt cttttttcat ccaggacaat acctttaccc
    61021 ttacaccctc tgcacgctgt gctgacttct cccttaccat gacaatgctg acacagttcc
    61081 ttcacccact cttccttgat aacagattcc ccgcttctgg agtgtttcac cacctcgcgc
    61141 aatacatcat gaaatccagt accagcacaa tgctcacagc gagccttact tgccgcagac
    61201 ctggaataat cagcaaaggc aaaattcaca aggtaaggaa tgatctgtag ccgggtttct
    61261 tcactcaatt tgttcaatgt cgggttatcc agtgccatcg cgtaattgag cagaccttca
    61321 atcgcaaact gaggatcctg aacaccaact tttgccagga ataaggcaaa cccaagcggt
    61381 gctttcgact gcaccatccc ctgcgcagcc attacatccg taattgttaa accaccagag
    61441 cctgtcgccg gtgcgtcatc gctcaatttt ggagattttg gggagtaata tttcggtaag
    61501 gcttcaaagt tcatgctcgt tctccactta cgccagtacg cctattgcca gcgcacgatc
    61561 gataaaacga aatatcagct ccagctggga gccatacttc tcttcaaatg ccacggtatc
    61621 cgcatgcagc tcgtcgtgat gctttctgca caaaggcaac acaaagaggt catgcgcttt
    61681 tgtacccatt cctccctgac cgtgacctat caggtggtgg ggatcatcag caggctttcc
    61741 acaacatgca cacggctgtg tcttaaccca gcgcgtgtac ttttcattaa cccagcggcg
    61801 acgttttggg cgtaacataa aagactccgg cgactcagga tccactttca gcgccagcac
    61861 ctttttcgct ttatcctgga tgatgctggt ggcaggaacc gaaggcacaa ggtcactttc
    61921 ccgggtaaca gacggcacaa caggcttcgg taatctaagt gccttacggg ctgcactttc
    61981 cggtaaggca tccgccaggt cattacgaat cagccaccag cacagttccg gcattgtcac
    62041 aacgtgactg tcatcaaaac cgagatcccg acgcacaaca gacaacaccc agcgggcaca
    62101 gttatccgtt gccattgatt ccagccgttc cgtgaactga tcgcgcagct ggttatcgca
    62161 gtgccagcac agacggattg cgcccggagc gtgtcgcatt gtggtcatgt tctcgctgtg
    62221 ccagtcggaa tgaggccact ggcagccttt ttcacgaagt aaccagcttt caagacattc
    62281 cacgccacca gcacgacgga tcactgcctc attgcggaac acggcccgaa cggcaggatc
    62341 atccgccagc ggttgtgatg ccgccggaac ggcaccactg gcgaaagatg aataacgttc
    62401 cggctcaggc tccagcagga cacgcccctg cataaacagg ggcatcagct ctgaacctgg
    62461 cctgaacaat acgatcccca tacgcggggc aatttcagga gtcagtagtg ctctcacggt
    62521 cacctcaatg aacggtatcg agcagcttta acagctcagg gaatcgggat tcgaagaaat
    62581 gcggctgcgt ctcgcgcgga tttgcgggac tggtgatgtt cttgccgaac atgcagcctt
    62641 tcgctgtcag cgaccagaat tttttgatgt tgttaatcgc agtgcggctg tatcgttcac
    62701 gttgttcaac gatccccagc ttcgccatct ggtgatatgc ctgattagct gtcaggcgga
    62761 taccatactg cttcagcagt gcactcaatg atagcgtggg gcggcttgag ccatcaggcg
    62821 cgtcagcagg agcatcaatg gcatagcgcg gtgccagatt cggtaagcca acagcctcct
    62881 ggagtttctg acaggcacca agcactgaag agttagacag gtttaactcc cggcgcataa
    62941 agtccagcag gatcacgcca gcctgcatct tgtcagcagc ctgtccggat aatttttccg
    63001 gtgcgctggt taccatatcg aaagtacgga tcaccttcag atggaatgac gggctgatcc
    63061 acattgcata ggcatacacc agttccttgc agacatacgt tccccgttca tttcccccat
    63121 gaatcacact caccgggtca acacccaaat tctgggtgtt ggtcaattca tgaacaagct
    63181 caacagtttg ttggctggaa agaaactttc ccggctcctt ggttctggca tttgcaccag
    63241 atgctactgc tgcgcgatgc agatcgttca ggctgtaacg cccataagca tcacgacgaa
    63301 cttcaatacc atcaatgacc atcagattat tcatacttcg tttctcctct taatcaggcg
    63361 gctgcacccg ccgttttctc gtacttactg atagtgatct cgaccttccc ttccgggata
    63421 accggtcccc actccaccag cattcttttc acctgactgt cgtcttccca cacacccgcg
    63481 tgggtcaggg cgtcaaacag cgccttgtta tagttgtcca gatcgcggat ccggttatcc
    63541 ggaggaaaca acacgatctc cactgaagca ggtgccgacg ttggtttcgg cagacgacgt
    63601 aactgctcaa ctattgctgc gcacgccgcg ctctggaatt ttcgccccgc cgcgcttatc
    63661 aggctcttac cagcaaacgc ccctttgttg gggtgtcgcc agtacgtgtt cacgctgggc
    63721 ggaaaaggca ggatcagctt catactttca ggtccctctc atgtaaccag tgggttgcac
    63781 gcagccttgc gttttcctca ccggcaagca gtgcgcggat aatcccgacc gcctcgctgt
    63841 cgtcgtcctt caccgcgata tgaagcgtta tcccccgggc cacgccacgc tttatcgtga
    63901 tgacgccttt tttctccagt gcgcgaagat gctccaccgc tgcattcact gaacggtatc
    63961 ccagcatggt tgccacctcc tgattggttg gcgggaagcc acgttctttc tgataagaaa
    64021 tcagcatatc cagcacctgc tgctggcatt gagttaacgt cgtcatgccg ccatctccct
    64081 gaccagtttt tctgcctgct ggcgaacctg cgccagaaag gcctcaccac atgcctcaag
    64141 ttcatagcgc ccgatgtagc tgattgccgg tcccttccag gtcttgtcga aaacagcaat
    64201 agcaccagcg aagaaagcgc ctgtcggcac ctgcttctca tccttcggga taaaccaggc
    64261 aggcagttca aaaccaatac gcccgcgaat aaaagcaata tgatctgcat cttccggcca
    64321 ccacacttcg ctggtggcag ctttgatcag gaaaacatag cgcccgcctt tatcacgcat
    64381 ggcactggca tgcttcatga tgtaacgcat gccggtgatg tattgcccct catgctgact
    64441 ggcgcggctg tatgggggat taccaaaggc agcaccttta agctccgcaa gacgttctga
    64501 ccagtcatgc gccagcgcgt tgtcttccgc agtgtaataa gcggcacatt tggcgttatc
    64561 accgtcagtg aacagatcca gaacaaacgg gccaaacagg gtgttaattc cccagaaaat
    64621 gttgtccggc gtgcgccact gatcgcccac ttccttcagt tcatgggctg gtttgttccg
    64681 cagctccacc agcgcctggc aatatttatt actcattaag cccccacgta attccctgac
    64741 agataccact cttcacccga tacagcgcgc ttgctgcttt tccgtaagca ccgctcacga
    64801 cgcgccagaa aattgtttcg ttctggctgg gagtggcttt cacggaatgc cgccatccac
    64861 accgttgcag cacgacggta taagcccctg gactccagtt cttcagcctg gcgggtcagg
    64921 cacaaaatca cccggggatc gttagtgccg acatagaaat tgcgcacagg tctggtttca
    64981 cgaactggtt gtggttccgg ctcctgcgct ctctcagtca ggcgcgggaa atgtctgcgt
    65041 gtatcccctt cacaacggtg agccacacgc ccactctgac gtaacttgct tgcagactgc
    65101 agaacgcgct gccgtgagta acctgcaaaa gcatccgcaa tgtctccgga agtacagccc
    65161 ggatgggctt caatgaattt ctgaacgtca ttcaaaagac tcatgctcac cccctgaatc
    65221 ctgccgggat ctggctgtag tccacgttgt cgtaactggc tttgaagtac gggtcctcgc
    65281 gtttttcggt gtacgtgctg acggacggcg ataagcgcag ggaaagctca tcccattttt
    65341 cccgcagctt cgacgggctg agcacgttac ggcaccagaa cggatcgcga ctgacgcggc
    65401 tgtacatctc gcagatttgt ttatgagtac gaccatcctg cacacacatc aggcgaattt
    65461 cgtttgccca ggctgtccag ttcggttctt tgggacgaac cacctcgccg tcacattcgg
    65521 cggcatgctc gtacagggcg atgatttttt tccagagcca ctgtgcacag gtcaaatcat
    65581 cctgcgtccc ccactggcgc tttttagggc tgaatacaac cgcatcagga tggcgagtta
    65641 aaaaatcctg ttcatccgtc tgcgtgtccg gttgcgaagc gtccggacga gaaggttttt
    65701 tatctgacgg atcatgtttt gattttactg acggatcccc gccagattct gacgggtgaa
    65761 aacccgcttt tttgccagat ttcgacgcat caaattttga cgggtcagat tttgatgcgt
    65821 cagattttga cgggtcagaa tctgacagtt gagaaaatgc cgctgcctga agcttcgcaa
    65881 cgttaagctg ataaacattc gacgcattgc ggttaccctg gcgacgcgcc ttacgcgtta
    65941 accagccttc tgcttccagc cgtgcgatag ccgttctgac ggtgctcatc cccgcgccaa
    66001 tctggcgggc aatggtttca attgatggcc agcacacacc ttcgtcatta ctgaaatcag
    66061 ccaggcgggc cataattgcc acgctggata acttcatgcc tgacgcagcg caaccatccc
    66121 atacatagcc ggttaattta gtgctcatga ccgacctcta tttccctgaa tttacgacga
    66181 aactgttcga gcgggctgaa gcactcatgc tcatagcctt cgcggaggta gataacccgt
    66241 tgtgtttccg gttcccaacg aatgactctg acgggcactc cgtagtgatc tttgaaccag
    66301 cggttaactt gtcgcaaagg actgtctcct tttgcctgtt gaaatcaccc acagcccact
    66361 ctgcaaagct gtgggttaca atttccctgt cacctggtac attcactgca tagcaatact
    66421 ccaccttcgc ttttccaccc ggtacaggaa gtgcaatcag ttgcgagcga cggtagtgtg
    66481 ttgttaaact gttcatgcgt tagtttctcc acagtcacga cacgccacgg cgcccggagc
    66541 tgcacactcg cgggcgtcat tactttctga aatgcaaaaa attttgtaga ccagtgctgc
    66601 atgctcctgc agcttcgaaa ttgagaggta cagctcgtcg ttaattgctg tcttctcatg
    66661 cggttccact acaccgtctt caattgctga acgaatctgt tttgaataac tgccgatctg
    66721 ttcaatgacc tccagcaggc gttggttgat atcggcgttg tccacatcct cgacatcagg
    66781 aagagacaca aagacgccat ttgcagactg cgccacagcg tcagcaatga agtgagttcc
    66841 accagcacgt tgcaaaatca ttgcccatcc cagcgggaaa atctgatcgc catcggcacg
    66901 aaggcggtta aataatgcgt tctctgttac atccagccag tcagcagctt cagcgtaccc
    66961 ccccggcaac gctgcgatag tttttctgac agctttcacg taccactcag gctgtttttc
    67021 tactttccag tgatgcttac ccacggttca cctcctgttc ctgtggttta aacccattct
    67081 ggttttggct agattgaaaa cgtgccggat aaagaatctg catttcgctg acttcaccct
    67141 taaaaaaatt ggctaaacgt tctgcaagct cgatagatgg aatctgctcc agcctctcaa
    67201 tacgactcaa cgtcgctgga ttgacttgaa cacccgcagc aacatgctgc aaagtgaaac
    67261 catgcgcctt acgcacattt cgtaatggtg attgcatacg ccctccaaat attgcgcgtt
    67321 atgcatgtta tttcacgcaa ttattttgcg caagttgatt tgcttatcac gcaataaaga
    67381 aatgtaataa acgcatgaac ataggaaacc gagtcagaca acttcgccaa gcgaagaaca
    67441 tgaaaatcgc cgatctcgct gaagcgatag gagtagatgc ggcgaacatc tcgcgcttag
    67501 aaacgggtaa gcaaaaacaa tttaccgaac aaacactgag taatattgcc aagagcttag
    67561 gtgttgatat tgctgatctc tttacctctg cccacaaaag taatactgta tataaaaaca
    67621 gtaataatga ggatgttgcg caggtgaagg atgtgttccg tattgaaatg ctggatatca
    67681 gtgccagtgc gggaaatggc cttatccagg gcggtgatgt cattgatgtg attcatgcca
    67741 tcgaatacag aactgataat gctgtatcaa tgttcggcgg acgaccagcc aatcacatca
    67801 aagttatcaa cgttcgtggg gacagtatgt gtccaaccat tgagccagga gatctcatct
    67861 tcgttgatgt cagcatcaat cagtttgatg gtgatggtat atatgtcttt ggttttgatg
    67921 acaaaatata cgttaaaaga cttcaaatga ttcctgacaa actgctggtg atttctgata
    67981 accagattta ccgtgaatgg ggaattacta gcgaaaacga acaccgattc atggtctttg
    68041 gaaaggtctt aatcagtcag tcgcaaaccc ttaagagaca taattaacct caatatccca
    68101 tccatcggcc accgaaaggt ggctttttat tacctataaa tttgcatatc tcgcaaatac
    68161 cacttgcata tctcgcaatt taattttatc ttttattcca gaccaactac aggattacaa
    68221 caaaatctgg ttgcaacacg gtgcatgtgt cgtaagcagt cagtaaatgt caaaaacgaa
    68281 caggcaggac gcccacgaag tagccgcctg gggcatatga agtccaggat gattcgttga
    68341 gtcatgttgt gccactaggc actcatgtta aagcaggtgt atgaaatgaa agtccagatt
    68401 ttaaacaata actgtgaagt cgtttggtca tacgacatag ccgcccctgt agatcagagc
    68461 ggcgatagct ggaccaatgg gaacatcaga ttatggctgg agttgtgttc tctttacgcc
    68521 gtgctttaga acaggctgaa gtctttccat cagaccctga atggaaatgg cctttttcta
    68581 tttgtccaaa ttcggagagt acatttcaga aaattggtca gaaagtcgca ctcgaagagc
    68641 atcagccaac tgtttcctga ttttttcagg taactcgtcg gcatcgcaga aacaacaacg
    68701 ctcgatcatg ttgaaagccg attcgtagaa ctgtttttgc tgagtgtcgc tgagacagga
    68761 aaagagcgac gttacgatga ttttattaat tgcattatca agttcttttt catcaaaagt
    68821 catttgattt tccttttatg tatacgggct taaaaggata ccaccgagcc tgaagtggtg
    68881 aaaagacagg cacataacag ctaagtattt tcaaccagag agaatcctta gcgttgtggt
    68941 gaatgcggct cagcgcacgc gggttaaggt tgaggctgac agtcgacctt ctgtggatac
    69001 ccacccgcct ggtgtgcaac cttcgccagg caccgggagg cacccggcac cacaacttta
    69061 tgctgtgtgt agtcttagcg gtaccagctt gtacccttgc ttccggctgg taccgtcctt
    69121 tttacaaaac agagaagagc atcaccggac gacgggctca taacccaatc catccgggcg
    69181 gctgccaccg caggtgttct tctctgtttt gtggagaaac taaccgcccc tacgggggca
    69241 tttatggaaa tgtaattgac tcaataatcg ccggacggtg agggcttcct tttacccgaa
    69301 ttcagcgcgg tgcagcgcat atacgtggag aacaaaatgt catttattaa aactttttcc
    69361 gggaagcatt tttattatga caggataaat aaagacgaca tcgttattaa cgatatcgcg
    69421 gtttcccttt caaatatctg tcgctttgca ggacatcttt cacacttcta cagtgtcgcc
    69481 caacatgcgg tgctttgcag ccagctggtg ccacaggaat ttgcttttga agctttaatg
    69541 catgatgcaa cagaagcata ttgccaggac atccccgcac cactgaaacg acttcttcct
    69601 gactataaac ggatggaaga aaaaatagac gccgtaatac gtgagaaata cgggttacct
    69661 cctgttatga gcacgccagt gaaatatgcc gatctcatta tgctggcaac cgaacgccgc
    69721 gatctcgggc ttgatgatgg ctctttctgg cctgtactgg aaggtatccc ggcaacagag
    69781 atgttcaaag tgattccaca ggcaccgggc catgcctacg ggatgtttat ggaacgtttt
    69841 aacgagttat cggagttacg caaatgcgca tgaatgtttt cgaaatggaa gggtttcttc
    69901 gtgggagatg tgtaccgcga gatctgaaag tgaatgaaac agatgctgaa tacctggtgc
    69961 gtaaattcga tgcgcttgaa gctaaatgtg cagcacagga aaacaaagta ataccagtgt
    70021 caactgaact gccaccagca aatgaaagtg ttttgttatt cgatgctaac ggagaaggct
    70081 ggctaattgg ctggcgttct ctctggtaca cctggggaca aaaagaaacc ggagaatggc
    70141 agtggacatt tcaggtcggg gaccttgaaa acgtcaatat cactcactgg gcagtaatgc
    70201 caaaagcacc ggaggctgga gcataatgac cacttttacc gacaaagaac tgattaaaga
    70261 aattaaagag cgtatcagca gccttgacgt gcgagacgat attgagcgcc gtgcttatga
    70321 aatcgcactc ctatctctgg aagtagaacc agatgaacgc gaagcttatg aattattcat
    70381 ggaaaagcgt ttcggtgact tagtagatcg tcggagagca aaaaacggcg ataacgaata
    70441 catggcatgg gatatgactc tcggttggat cgtctggcag caacgagcag gtatccattt
    70501 ttcaacaatg tcacaacaag aggtgaaata atggagccat acagcctcac actcgatgag
    70561 gcctgtcatt ttctcaagat atccagaccg actgccatta actggatacg cacagggcgt
    70621 cttcaggcaa cacgcaaaga tcccactaag aataaatctc cttacctcac aacacgacaa
    70681 gcctgcattg cggctcttca gtctccgctg catactgtcc aggtgagcgc gggtgatggc
    70741 ataacagagg aaagaaaatg tcactcttcc gcagaggtga aatatggtac gccagtttca
    70801 cattgccgaa cggtaaaaga tttaaacagt ctcttggaac aaaggacaaa aggcaggcga
    70861 cagaactcca tgacaagcta aaggctgaag catggcgggt cagcaaactt ggtgaaatac
    70921 ctgatataac gttcgaggaa gcgtgtgtca ggtggcttga agagaaagca cataaaaaat
    70981 cgctggacga tgacaaaagc cggatcggat tctggcttca acatttcgca ggaatgcaac
    71041 taagagacat tactgaatca aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc
    71101 atgaggaaaa ctggaaactc agggcagaag catgcagaaa aaaagggaaa cctgttccag
    71161 aatacacgcc aaaaccagcg tccgttgcaa cgaaggctac gcatctttca tttataaagg
    71221 ccctactaag agccgcagag cgtgaatgga aaatgctgga taaggcacca attattaaag
    71281 tgcctcaacc aaagaataaa cggatccgct ggctggagcc ccatgaagca caaaggctga
    71341 ttgatgaatg tccggagcca ttaaagtctg ttgttgaatt tgcactggca acaggcttaa
    71401 gacgctcgaa catcatcaac cttgaatggc aacaaataga tatgcagcgc cgggtggcat
    71461 ggataaaccc ggaagagagt aaatcaaacc gcgcaattgg cgttgcgctg aatgatactg
    71521 catgtcgcgt attgaaaaaa caaatcggga atcatcaccg ttgggtattt gtgtacaagg
    71581 aaagctgtac caaaccagac ggaacgaaag cgccaacagt aaggaagatg cggtatgacg
    71641 caaacacagc ctggaaagcg gcgctgagac gggctggtat tgatgatttc agatttcacg
    71701 acttgagaca cacctgggca agttggctgg ttcaagccgg agtcccgttg tcagtgttac
    71761 aggaaatggg aggctgggag tctatcgaaa tggttcgtcg atatgctcac cttgcaccta
    71821 atcaccttac cgaacacgca cggcaaatag actcgatcct gaacccatcg gtcccaaatt
    71881 tgtcccagtc aaaaaataag gaaggtacta atgatgtgta acttattgat tttaatggtg
    71941 ccgataatag gagtcgaacc tacgaccttc gcattacgaa tgcgctgctc taccaactga
    72001 gctatatcgg ccctgaaagg acatgttcac gaacgtgaat cacggtggac aaggttaaaa
    72061 ctaaccgggc gatgcgtcaa tggccttgtg aatcaaacgg ctacttttgc atcacccggt
    72121 tttatttacg cacgaatggt gtaatcacca atgccgatcc acttgtaagt ggtcagtgct
    72181 tccagcccca ttgggccacg cgcgtggagt ttttgtgtgc ttaccgccac ttccgcaccc
    72241 agtccaaact ggccgccgtc ggtaaaacgc gtagaggcgt taacgtaaac agcggacgaa
    72301 tccacttcgt taacaaaacg ctgggcgttg cgcatatcgc gggtcaggat cgcatcggag
    72361 tgttgcgtgc cgtgttcacg aatatgggcg atggcatcgt caagatcgct gacgattttg
    72421 acgttcaaat ctaatgacag aaactcatcg tcatactctt cggctttaac cgccaccacc
    72481 ttcgcagggc ctgtctgcaa ctgcgccagc gcagatgcat ctgcgtgtaa cgtcacgccg
    72541 ctttccgcca tttgtttgct taatgcgggc aggaagctat aggcgatgtt tttattcacc
    72601 agcaacgttt ctaccgtatt acatgtgctc ggacgctgag ttttcgcatt gacgattact
    72661 ttcagggctt cagcaatctc tgcactttca tcaacataaa tatggcatac gcctatacca
    72721 cctgtgatca ccgggatcgt cgactgctcg cggcacagtt tatgcaaacc agccccgccg
    72781 cgcgggatca gcatgtcgat gtatttatcc atacgcagca tttcactgac cagcgcacgg
    72841 tcaggattat caatcgcctg cacggcaccc gccggtaagc cacaggattt cagggcgtcc
    72901 tgaatcacca ccaccgttgc cgcgttagtg cgacaggttt ctttgccacc acgcaggatc
    72961 accgcgttac cggttttcag gcacagcgaa gcgacatcaa ccgtcacgtt cgggcgcgct
    73021 tcataaatca cgccaataac ccccagcggt acgcgacgac gctcaagacg caggccgctg
    73081 tccagtacgc cgccatcgat tacctgcccc actggatcgg cgaggttgca cacctgacgt
    73141 acatcgtcgg caatgccttt cagccgtgcg ggcgtcagtg ccagacggtc aagcatcgct
    73201 tcgctaaggc cattggctcg cgcgtcagca acatcctggg cgttagcgtt gaggatgatt
    73261 tcgctttgtg cttccagttc atcggcgatt ttttccagca cgcgattttt ttcgcggctg
    73321 gagagttgcg ctaatttata cgaggcttgc ttcgcggcaa tgcccatttg ttccagcatc
    73381 agcctgctcc ttaacgggtg atcatgtcat cacggtgaac ggcaaccggg ccgtattcat
    73441 atcccagtat tgcatcaatt tcttgcgagt ggtgtccggc aatacggcgt aatgcatcgc
    73501 tgttgtaacg actgacgccg tgggcgatat cgcgaccttc gaggttacaa atgcggatga
    73561 cttcaccacg cgagaagttg ccagtcacgc ttttaatgcc tttcggcaac agggagctgc
    73621 cgcgttcaag aatggcggcg gttgcccctt catctaccgt gatttcaccc gcaggcggcg
    73681 caccgaaaat ccagcgttta cggttttcaa gcggagtcgc ctgggcatgg aacagcgtac
    73741 cgacggaaat gccttccatc acatcaccaa taacgcccgg cttgctgccc gcggcaataa
    73801 tggtgtcgat acccgcacgg caagccacgt cagcggcctg caatttggta ctcatgccgc
    73861 cagttccgag gcctgaaacg ctgtcaccag caatcgcgcg cagtgcgtca tcaatgccgt
    73921 aaacatcttt aatcagttct gcctgcggat tgctgcgcgg gtcagcggta tacaaacctt
    73981 tttgatcggt cagtaacaac agtttatcgg cacccgccag aatcgccgcc agtgcagaaa
    74041 ggttgtcgtt atcgccgact ttaatctctg ccgtagcgac agcatcgttc tcattgatta
    74101 ccggaacgat attgttatcg agcaacgcac gcagggtgtc gcgggcgttc aggaagcgtt
    74161 cacggtcttc catatcagca cgagtcagca gcatttgccc gacgtgaatg ccataaatcg
    74221 aaaacagctg ttcccacagt tgaatcagtc gactctgccc taccgccgcc agcagttgtt
    74281 tcgaggcgat agtcgctggc agttccgggt aacccaggtg ctcacgtccg gcggcgatcg
    74341 cgcccgacgt cacaataaca atccgatgcc cggcggcatg taactgcgcg cactggcgaa
    74401 caagttcaac gatatgggca cggttcaggc ggcgcgatcc gcctgttagc acactggtgc
    74461 cgagttttac caccagcgtc tggctgtcac tcatgattct ctgccattca attttaggaa
    74521 aaatgatatc aaacgaacgt tttagcagga ctgtcgtcgg ttgccaacca tctgcgagca
    74581 aagcatggcg ttttgttgcg cgggatcagc aagcctagcg gcagttgttt acgcttttat
    74641 tacagattta ataaattacc acattttaag aatatcatta atctgtaata tatctttaac
    74701 aatctcaggt taaaaacttt cctgttttca acggggctct cccgcagaat attcgcgcgt
    74761 taattaaaat caggaatgaa aatgaaaaag agcactctgg cattagtggt gatgggcatt
    74821 gtggcatctg catccgtaca ggccgcagaa atatataaca aagacggtaa taaactggat
    74881 gtctatggca aagttaaagc catgcattat atgagtgata acgacagtaa agatggcgac
    74941 cagagttata tccgttttgg ttttaaaggc gaaacacaaa ttaacgatca actgactggt
    75001 tatggtcgtt gggaagcgga gtttgccgga aataaagcgg agagtgatac tgcacagcaa
    75061 aaaacgcgtc tcgcttttgc cggattgaag tataaagatt tgggttcttt cgactatggc
    75121 cgtaacctgg gcgcgttgta tgacgtggaa gcctggaccg atatgttccc ggaatttggt
    75181 ggcgactcct cggcgcagac cgacaacttt atgaccaaac gcgccagcgg tctggcgacg
    75241 tatcggaaca ccgacttctt cggcgttatc gatggcctga acttaaccct gcaatatcaa
    75301 gggaaaaacg aaaaccgcga cgttaaaaag caaaacggcg atggcttcgg cacgtcattg
    75361 acatatgact ttggcggcag cgatttcgcc attagtgggg cctataccaa ctcagatcgc
    75421 accaacgagc agaacctgca aagccgtggc acaggcaagc gtgcagaagc ttgggctaca
    75481 ggtctgaaat acgatgccaa taatatttat ctggcaactt tttattctga aacacgcaaa
    75541 atgacgccaa taactggcgg ctttgccaat aagacacaga actttgaagc ggtcgctcaa
    75601 taccagtttg actttggtct gcgtccatcg ctgggttatg tcttatcgaa agggaaagat
    75661 attgaaggta tcggtgatga agatctggtc aattatatcg atgtcggggc tacatattat
    75721 ttcaacaaaa atatgtcagc gtttgttgat tataaaatca accaactgga tagcgataac
    75781 aaattgaata ttaataatga tgatattgtc gcggttggca tgacctatca gttttaatga
    75841 ttattgccgg atgtgatgca tccggcacat ttcactcacg ccgttaactt caccggctcg
    75901 tcacgaaaat catccgccgg ttccaacttc agattcagcg tcgtcagcag ttcacgcagc
    75961 ttctcgtgaa actcacgcag ggtgtgctcc agtcgttcaa ccacttcagt atcttttacc
    76021 ggaacactct tccagtcgcc tgctttatcg aacagaccaa actggtaact gtaggtaaaa
    76081 cgggattcct gcgcttcaag ctccatccac cagccccaga actcacgcac ttccggtgcc
    76141 ggtttcacgt tgacgcatac agccagacaa tcgaaaaaga atcgattatc ttcgcactta
    76201 ccttcacgaa tatacgggcc tagtacggta aattttttga tcaatctgct cttcgggtgt
    76261 ccactcggta acgtcattgc tatctcctgt tgtgatgcaa ctgttttacc aaattggcaa
    76321 aatttagcaa atttttaaca caagcgtttt tcgatccagt cggtgatttc ctgaagacct
    76381 ttgtcaaaat tccgatacac cgggttaaat gggatctcta ataatttacc gtcagcagat
    76441 gatgaggtga ttaagcgtga gtcctcttcc gggctgaacg gatcgttctt ccagtagcct
    76501 gataacattg gcgttgggca tcgacgtccc agcaatcctt gcacttttaa tgaatagcga
    76561 ttcagctcca cgcgcaacgc ttcgtcggaa gcatcatgca tccccaaacg actcgccaga
    76621 acgtcaagat acatttccgg cacctgttgc tggcacttaa aatcactcaa cagggtatga
    76681 actaccggac caagacaggc aaccgctttc agacgcggcg attcaagata tgccagacgc
    76741 acggcaacgt tagcgccgaa acggaaacca aaggccgcga cgcgagtgtg atccacccac
    76801 ggtacgttag gcagagcctt taagacgcgc tgatgcaaca ggcaggagtc ctgggtgagc
    76861 ttccattttg aagaaaagcc caccgacggc atatcaatag tcagcatcgc aatgccgcgc
    76921 ggcgcaaaat aacgttcata caggctgtaa tagtccgtct gcatcgcatc cagaccacca
    76981 cacattaata ccgtcgggaa cgggccatcg cctttcggca tatgcaaaaa gccggtgatg
    77041 ggcgcaccgc cgggtacggt aaactccatc tgtcgcatcg tgcccggtag acgctgagcg
    77101 gcctcttcat aggcgcggtt tgacaaagcc tgcgcttgct cggccaggtc atctcctttc
    77161 agatgaggat aggcggcaat gttgtacaac gtagccgcat gcagccagtg acgaccactg
    77221 agttgcggat cttcttccgc acaggctttt tgttgccaca ccatcgcctg ggtggcccac
    77281 tcataaatcc agttgccgcc acggtagcca atcaccgtat cgtataaatc atcgtcggta
    77341 cgttcggcat cgctcatcac aatacgtgcc tggacgtcga ggatttcgcg tgggtcaatg
    77401 ccgcgccaga tccacatcag acggttaatc atgcgatacc agtgagggat ggttttacca
    77461 tcaagggccg actgcacagg cggttgtgcg ccgtgattaa agcggcggac tagcgtcgag
    77521 gtttcaggat gtttaaagcg gggtttgaac agggtttcgc tcaggtttgc ctgtgtcatg
    77581 gatgcagcct ccagaatact tactggaaac tattgtaact cgcctgaagt taaaaagaac
    77641 aacgcccggc agtgccaggc gttgaaaaga ttagcgaccg gagattggcg ggacgaatac
    77701 gacgcccata tcccacggct gttcgatcca ggtatcttgc gggatatcaa caacataatc
    77761 atcaaccagc ggacgaccag ccggttttgc aaagatggtg acaaagtgcg cttttggata
    77821 catttcacga atcgcaaccg cagtaccacc ggtatccacc aggtcatcaa taacgatgaa
    77881 gccttcgcca tcgccttctg cgcgtttcag cactttaagc tcgcgctggt tgtcgtgatc
    77941 gtagctggaa atacaaacgg tatcgacatg acgaataccc agttcacgcg ccagtaacgc
    78001 acccggtacc agaccgccac ggcttacggc aataatgcct ttccattgtt cagaaggcat
    78061 cagtcggctt gcgagtttac gtgcatggat ctgcaacatg tcccaggtga cgatgtattt
    78121 ttcgctcatg tgaagtgtcc cagcctgttt atctacggct taaaaagtgt tcgaggggaa
    78181 aataggttgc gcgagattat agagatctgg cgcactaaaa accagtattt cacatgagtc
    78241 cgcgtctttt tacgcactgc ctctccctga cgcgggataa agtggtattc tcaaacatat
    78301 ctcgcaagcc tgtcttgtgt tgacaacatt ttctgctaac cctgtgacct gcaatactgt
    78361 tttgcgggtg atcgacaagg agacttaacg tgtctgaact gtctcaatta tctccacagc
    78421 cgctgtggga tatttttgcc aaaatctgtt ctattcctca cccgtcctat catgaagagc
    78481 aactcgctga atacattgtt ggttgggcaa aagagaaagg tttccatgtc gaacgcgatc
    78541 aggtaggtaa tatcctgatt cgtaaacctg ctaccgcagg tatggaaaat cgtaaaccgg
    78601 tcgtcttgca ggcccacctc gatatggtgc cgcagaaaaa taacgacacc gtgcatgact
    78661 tcacgaaaga tcctatccag ccatatattg atggcgaatg ggttaaagcg cgcggcacca
    78721 cgctgggtgc ggataacggc attggtatgg cctctgcact ggcggttctg gctgacgaaa
    78781 acgtggttca cggcccgctg gaagtgctgc tgaccatgac cgaagaagcc ggtatggacg
    78841 gtgcgttcgg cttacagagc aactggttgc aggctgatat tctgattaac accgactccg
    78901 aagaagaagg tgaaatctac atgggttgcg cggggggtat cgacttcacc tccaacctgc
    78961 atttagatcg tgaagcggtt ccagctggtt ttgaaacctt caagttaacc ttaaaaggtc
    79021 tgaaaggcgg tcactccggc ggggaaatcc acgttggcct gggtaatgcc aacaaactgc
    79081 tggtgcgctt cctggcgggt catgcagaag aactggacct gcgtcttatt gatttcaacg
    79141 gcggcacact gcgtaacgcc atcccgcgtg aagcctttgc gaccattgct gtcgcagctg
    79201 ataaagtcga cgccctgaaa tctctggtga atacctatca ggagatcctg aaaaacgagc
    79261 tggcagagaa agagaaaaat ctggccttgt tgctggactc tgtagcgaac gataaagctg
    79321 ccctgattgc gaaatctcgc gataccttta ttcgtctgct gaacgccacc ccgaacggtg
    79381 tgatccgcaa ctccgacgtg gcaaaaggtg tggtcgaaac ctccctgaac gtcggtgtgg
    79441 tgaccatgac tgacaataac gtagaaattc actgtctgat ccgttctctg atcgacagcg
    79501 gtaaagacta cgtggtgagc atgctggatt cgctgggtaa actggctggc gcgaaaaccg
    79561 aagcgaaagg cgcatatcct ggctggcagc cggacgctaa ttctccggtg atgcatctgg
    79621 tacgtgaaac ctatcagcgc ctgttcaaca agacgccgaa catccagatt atccacgcgg
    79681 gcctggaatg tggtctgttc aaaaaaccgt atccggaaat ggacatggtt tctatcgggc
    79741 caacaatcac cggtccacac tctccggatg agcaagttca catcgaaagc gtaggtcatt
    79801 actggacact gctgactgaa ctgctgaaag aaattccggc gaagtaatta tttgatttgc
    79861 tgccggatgg cgtttaatcg ccttccggca gtttcatcct tcattatcct tcgataaaag
    79921 ccatccctgt gaacgttcgt cgcgggttgc cacgttcaat ctggtgatgg aacattcgcc
    79981 gctgcgattt cagcactgcg ctattttcct gttgctgctg ctccagcttc caggcaatca
    80041 gcaatcgcgc cagccgtttg ttagcatgct ggctgcgttc tgactgaacc ttcacgctaa
    80101 tcccggttgc cagatgcgtg gcgcgtaccg ccgagtcggt tttattgaca tgttgaccgc
    80161 ccggccccga cggacgcagc gtctcataac ggattgcatc cgattgttcc tgctcatcag
    80221 cggtaaaacg cccaatgccc agaaaccagt ttttgcgccc atgatgaggc cgatacggac
    80281 tcagacaaat ccactgaata gtgccgcacc aactttcgct taacgcccat gcgttgtcgc
    80341 catccagaga aatcagcgcc gaacgcagcg tgtcagagta gcggcccgtt tctgtttcca
    80401 gcaccgttac cgcgacgtct tgtcgggtaa aaacgaccaa cagagaagaa cgctgcgcca
    80461 atcgggtagc cgcgtccggg gtgtaaatct ggcatcccca ctacgcgctg catgttgggt
    80521 aaatttgccg tggtgtgtaa ttgttggatc gctaaactct cgatccacag gtcatcagat
    80581 gcgatggtaa ataccgcatc ggataaggga cgaatatatt tgcccatgta ccattccatt
    80641 tatactcgtc atacttcaag ttgcatgtac tgcgactgcg ttcgttcacc ccagtcactt
    80701 acttatgtaa gctcctgggg attcactcgc ttgtcgcctt cctgcaactc gaattattta
    80761 gagtatataa atataaaaat caggaaatgg cagacgaatc cagggcaaga aaaaaaagaa
    80821 ttaatcctgg aaaggtctgc aaagagtcat tgataaagga tttgtcatga tttacctcct
    80881 tgcagtttgt ggaataagaa aacgcgcaca taccaggcgg gcgttatttt cattgcaagc
    80941 tggatttaat gttgcggttt atatcgcata gaaaaattag taaaccacgc tccccggcat
    81001 tcaacgcgct gctgcttaac tgtctgaaaa ccataacgtt caaaaaaggg ttttgcggtt
    81061 atgctggcgt ccaccgtaag ttcggattca gacttaatcc aaggttttaa caaagcgctg
    81121 gcaaccccac ggcgggtgta ttcagggtca acaaataaca tatcgatata atgttcaatg
    81181 cgggaaataa aaccaaccgg ttgtgcatta atgactgcaa cccgcacttg tgatttcgcg
    81241 agtttctcct tccagcgaga ttcgtcaatc tgcgcccagg cggcaatttg ttgtggtgaa
    81301 taatgctgac tggcggtcat cataaccgct ctaatgaaaa tagcgcatag ttgctgaaaa
    81361 tcgccaggct gatagtttct tatttgtatg ttattcataa tataaattca aaaacgcatg
    81421 cgaaacgctt ctgccatttt cccaatttta tgcatttggt tgttatctcg agccagttta
    81481 tgaggttcag gtttcagaat ggcaatgagc aaccatgctt gctcatcaaa cgcaccctga
    81541 caatatacca aatgcgattc gtcattcgtt ctgctgaatt ggcgcaactg tggcggaaat
    81601 ggattattca catttgccag atgaatatga gcaactcgct caaatttgat taatggccaa
    81661 gtaaacgagt cgtcgtagag tgcatcgcga ccaaatatat ctggcaaaac accgtcacgc
    81721 ttataggaaa taaaatccgc cgttaacgca tcaagctcct ctgctgtaag ttgcaggcga
    81781 ataagttttg ttttgaatac ccgcatcctt attccttaaa gtcattgaaa tcatcatccg
    81841 tcatatcatt aagatatttt tcagctcggc gtgtaatttc ctctaatctt gcagcacttg
    81901 gacggaagcg cgcctttatg ggctttttct cctcttgttc agcaagcatg tccattaacg
    81961 cttcgaatgc gctggcactt aagagatatc ctgcggggcg attattagaa agaaccgcaa
    82021 ccggttgatc aataaagtat ttagctgggt ttttacgtaa ttcagtgata ttgaccgatt
    82081 tttcagcgag aattcgatgc atacagtgat cccctcataa taatgcacac cagaatatac
    82141 ataatagtat acatcataat cccagcacca gttgtctttc catttgaggg tcaagcaacg
    82201 tcacatgcag ccccaccagt cgcacaccgc gcccgccgcg gcgttcatcc caggttttac
    82261 gcgcggtggc gattaagtca gctttattca gccgcggcca gacgtgctcc tgggtggttt
    82321 gctgaaaatc gtcgaacttt aatttcaccc cctggcgagc aatcagtaaa tcaggtttta
    82381 cctttgccag acggcgttca agttccggat acagccgctc gataatcgct tcacattcag
    82441 accagtgatg aatatcttcc gccatcgtgc gttccacgcc gacggatttt cgtaaccgtt
    82501 cgctgttaac gtcgcgctcg tcaatcccct gactacgctc ccacaaaatg cggccaaatt
    82561 tgccaaagcg tttaagcaga atcaccagat cacacttttg tacatcaccg caggtccgca
    82621 gccccatcgc ttccagcttt gccgctgaga ctttgccaac accgggaatt tttgccagcg
    82681 gtaaggtttg cagaaatgcc gaaacttctg ccggcgtaat cacaaactgg ccgttgggtt
    82741 tattcatgtc ggaggcgatt ttggcgagaa actttactgg tgccacgccc gcagacgccg
    82801 tcagttgcag ctcgctgaaa attgtctggc ggatttcctg ggcgatgagg gtcgcagaac
    82861 cgtggcaatg gacgctatca gtgacatcga ggtaggcttc atccagtgac aacggttcaa
    82921 tacgcgaggt gtagcgcgag aagatttcac ggatatgatt tgaggcttct ttgtaggcgt
    82981 caaagcgccc cggaagcaag gtgagatgtg ggcataattt gagcgccatc cctgtcggca
    83041 tagcgctgcg tacgccaaat ttacgcgcgg gataattggc ggtgctgatc accccccgac
    83101 gttcgcggct gccgccaata gcaataggga tatcgcgcag ggcgggattg tcgcgcatct
    83161 ccactgcggc gaaaaagcag tccatatcca catgaatgat tttacgcatt actcacctct
    83221 caacactggt aaagtataca gtgatttcag ggtttgagaa atgcgtaaag attcagcatt
    83281 tagcgcgttc gctgcgaaag cacctgctgc ttatccagct tttgcaccag cggctgcacc
    83341 actttatccc catgctgacc aaagtattga tacagcgtat cggacgcact tttggtcagc
    83401 accataatct caatgcgtcg gttgcccgcg ctttgcggat ttttcgcgtc cagcagcatc
    83461 tggtccgcca ttgcgcttac ctgcatcact ttatcttccg gcattccggc ctcttccagc
    83521 acccgacgag ctgaaagcgc gcgatcgccc gaaaggttcc agttgttgta gatattgttt
    83581 ttgtaggcca tcgcatcggt atgcccggta ataataattt tgttatcgag cgagtcgaac
    83641 actggcgcga gctccaccag caaagtttta aagaacggca taatctgcgc gctgccgcgt
    83701 tcgaacatat tgcgattctg gtcgtcttta atcagcacgc gtaatccctg cgggacaatc
    83761 tccatctcca gattcgcttc catatgcgca tcacgggcaa tggtgttgat actggttgcc
    83821 agctcgccaa gctcggtggc ggatttcttc ttcagcagcg ccgttttctc attcacatca
    83881 cgcgcttttt tctccgtctc ttccggcacc gccacggtgg caggttttgg cggatgagac
    83941 gggctgattt tatttaacgg cgacagccca ccaccgttaa aaatcgactg cccgtgcagc
    84001 gcggcgataa tgctttcacg ctcggattta ctgacgctgt tgacaatcaa ctttatcgtc
    84061 gtcaccaaag gggccgagcg tatttccgag gtttctgccc gctttaccct ggatgcgatg
    84121 cccggcaaac agatggcgat tgacgccgat cttaacgccg gattgatcaa ccaggcacag
    84181 gcgcaaaccc ggcgtaaaga tgttgccagc gaggccgatt tctacggcgc gatggacggg
    84241 gcatcgaagt ttgtgcgcgg ggatgccatc gccgggatga tgattctggc gattaacctg
    84301 atcggcggcg tctgtatcgg gatatttaaa tacaacctga gcgccgacgc cgccttccag
    84361 caatatgtgc tgatgaccat cggcgatggg ctggtggcgc agatcccttc cctcctgctc
    84421 tccaccgcag cggcgattat cgtcacccgc gtcagcgaca acggcgatat cgcccatgac
    84481 gtgcgccacc agctgctggc aagcccgtcg gtgctctaca ccgccaccgg gattatgttt
    84541 gtgctggcgg tggtgccggg aatgccgcac ctgccgtttt tgctgttcag cgccctgctg
    84601 ggttttaccg gctggcggat gagcaaacag ccgcaggcgg cggaggcgga agagaaaagc
    84661 ctcgaaacgc tgacccgcac catcactgaa accagcgagc aacaggtcag ttgggaaacc
    84721 attccgctta tcgagcctat cagtttaagc ctcggctaca agctggtggc gctggtcgat
    84781 aaagcgcagg gcaacccgct cacccagcgt attcgcggcg tacggcaggt gatatccgac
    84841 ggtaacggcg tgctgctgcc ggagatccgc attcgggaaa acttccgcct caagcccagc
    84901 cagtacgcca ttttcatcaa cggcattaag gccgatgaag cggatattcc ggcggataaa
    84961 ctgatggcgc tgccctccag cgaaacctac ggcgagattg acggcgtgct ggggaacgac
    85021 ccggcgtacg ggatgccggt gacctggatc cagcccgcgc agaaggcgaa ggcgctgaat
    85081 atggggtatc aggtgatcga cagcgccagc gtgattgcca cgcatgtgaa caagattgtg
    85141 cgcagctata ttcctgattt gtttaattat gatgacatca cgcagctgca taaccgcctg
    85201 tcgtcgatgg ccccgcgcct ggcggaagat ttgagcgcgg cgctgaatta cagccagttg
    85261 ctgaaagtgt accgtgcgct gctgaccgaa ggcgtttccc tgcgcgatat cgtcaccatc
    85321 gccaccgtgc tggtcgccag tagcgcggtg actaaagatc atattctgct ggcggccgat
    85381 gtgcgcctgg cgctgcggcg cagcattacc catccgttcg ttcgcaagca ggagctgacg
    85441 gtgtatacgc tgaataatga gctggaaaat ctgctgacta acgtagtgaa tcaggcgcaa
    85501 cagggcggta aagtgatgct cgacagcgtg ccagtggacc cgaatatgct caaccagttc
    85561 cagagcacga tgccgcaggt gaaagagcag atgaaagcgg cggggaaaga cccggtgctg
    85621 ctggtgccgc cgcagctgcg ccctttgctg gcgcgctatg caaggttgtt tgcgccgggg
    85681 ctgcatgtgc tgtcgtataa cgaagtgccg gatgagctgg agttgaagat aatgggggcg
    85741 ttgagttgat tggcctttaa ggggcgcatc cggcattggt gcacaattgc cggatgcggc
    85801 gtgaacgcct tatccggcct acggttgagt gcagttttgt aggcatgata agacgcgcca
    85861 gcgtcgcatc aggcatccgg caccagcgca caattatgaa atgatacgct ccccggcact
    85921 aaaatccgtt atgtctcccg cccaatcaat gggatataac cctcgcgcca catcgcgatg
    85981 gaacgttgag aatggccaat cactcacttg ctttacccag ccatgtttta ctggatttat
    86041 ataaatataa tcaacatgat ggcgatagtc tttggtattg cggatggtgt gctcctaaaa
    86101 acgtggttgc cagatatttt tcaatccaca agcatgggtg aactgctttt taatctcccg
    86161 ccagcgggag gaaaaatcat catcgctttc gggtaatgtc catatgcaat gcatatgctc
    86221 cggcaaaacg acccaggcgt taatttcaaa aggtctgtct cgcttaacgt taataatggc
    86281 gttacggagc atctggaact gagcggtcag aaggtggctt cgacgatttc gtaggttaac
    86341 cgtgaaaaac caggttcccc ctttgatgta ataccgacga tattcagaca tggcaactcc
    86401 tttttctcaa cggtaagaag aaaaggaaag gtcggcaatg aggtgatgga ataacgggaa
    86461 agcggctcgc aaaaacgcac agatttgtag gcatgataag acgcgtcagc gtcgtatcag
    86521 gcatctgcgc acaactgccg gatgcggcgt gaatgcctta tccgtcctac aaaatcgtct
    86581 cttccgtcaa aatccggcgc gcgcctaaaa agtgatcctg ccagaaaggt tcagctaacc
    86641 gacttacccg aatggtttcc ccggtgcgcg gtgactcaat aaactgaccg tcgcccagat
    86701 acacccccat atggtcggca atctcgcggc tgtggatatg gaaaaacagc aaatctcccc
    86761 ggcgcaggtc gttattcgcc acaatcgttg cccgatggta gtgatacatc tcattagccg
    86821 tacgcgggag cttcgcctcg aggatcttgt tgtaggcata aaaaaccaac ccgctacagt
    86881 caaagccttt atcaggccgc gtgccgcccc agacgtaagg cttgccaagc tgctgctcca
    86941 ggcggtgaat cgccacttcg gtaatgttat gcagatggtc gctgcttaaa aagtggttgt
    87001 tttccgccag cgcctgccag cgtctgtcac tcgccttgag cggtgcagga aaccattctg
    87061 gatgctgttt tatctgttcg cggttgtgct ggcgtagcgc ccttctgctt tcggcattgc
    87121 cttccacaat atagctgacc tgctttttca gatgggtttg gtactgtttt aataaacgcg
    87181 cacggttttg catccgctgg cgggcggcgt agctgaaaga gatatgtgat gtggtggcgc
    87241 tggcaaatga agaaaacggc aaacaaatca acagtaatcc cgacaataca aaggatggaa
    87301 tcgacggcaa ggacatactc aataatggct ggtcatgcat tgatgttaca gtgtatcccg
    87361 gcccgtacct gaacaaactt aataaaccat tattataaaa ttaataaata tatttcagtt
    87421 aattatgtaa aaacatccgt ttattaaatt actcatagat tgtcgctgtt gctcatttga
    87481 gctacaattc aagctgaata aatatacagc ataggagata tcccaatggc tgctaacgca
    87541 tttgttcgcg cccgaatcga tgaagatctg aagaatcagg cggcggacgt actggccggg
    87601 atggggctga ccatctctga cctggttcgc ataaccctca caaaggtcgc gcgtgaaaag
    87661 gcattgccgt ttgatttacg cgagcctaat caattaacca ttcaatcaat caaaaacagc
    87721 gaagctggcg ttgatgttca taaggccaaa gacgccgatg atttatttga taaattagga
    87781 gtttaaatat gattcaaagg gatattgaac tcgggacaat tttcaaagga tgtaaaactt
    87841 gcacaaaagc gtcataagga tatgaataaa ttgaaatatc ttatgacgct tcttatcaat
    87901 aatactttac cgcttccagc tgtttataaa gaccacccgc tgcaaggttc atggaaaggt
    87961 tatcgcgatg ctcatgtcga accggactgg atcctgattt acaaacttac cgataaactt
    88021 ttacgatttg agagaactgg aactcacgcg gcgctctttg ggtaaagccc gctgccgatg
    88081 aaatttgtaa accctgcgcc gacaggcttg cgtaaaactc ttttggtgat aatgtgagcc
    88141 ttcccaatcc agactattcc gatttgggat ccattttgcc gtcctggcgt gtgtaaccgt
    88201 tttatcaagg aataagcagt atgcgtaaaa tcgcattaat tcttgcgatg ttgttgatcc
    88261 cgtgcgtttc gtttgccggt ttgctgggca gcagtagctc gaccacgcca gtgagcaaag
    88321 agtataagca gcagttgatg ggatcccctg tctacatcca gatcttcaag gaagaacgta
    88381 cgctcgatct ctacgtcaaa atgggcgagc aatatcaact gctcgacagc tataaaatct
    88441 gtaaatattc cggcggctta gggccaaaac agcgtcaggg cgatttcaaa agcccggaag
    88501 ggttttatag cgtccagcgt aatcagttaa aaccagacag ccgttactac aaagcgatta
    88561 atattggttt ccccaatgcc tatgaccgtg cgcatggtta tgaagggaaa tacctgatga
    88621 tccacggcga ttgtgtttcc atcggctgct acgcgatgac caatcagggc attgacgaga
    88681 ttttccagtt cgttactggc gcactggtat ttggtcagcc gagcgtgcaa gtgagcattt
    88741 acccgttccg catgaccgac gccaatatga agcgccataa atattccaac tttaaggact
    88801 tctgggagca actgaagcca ggctacgact actttgagca aacccgtaag ccaccgaccg
    88861 tttctgtggt caatggccgt tacgtggtca gcaagccgtt aagccacgaa gttgtgcaac
    88921 cacaactggc atcaaactac acgctccccg aggcaaaata agcgccattc gcctggcata
    88981 atcttttgcc aggtttcatt gcccgtcagc ggctgtgtcg caatcaccgt gaccacatca
    89041 ttcggtgtgg tctgcgagct gaagtcgatt tccacatcct gatccagcaa cgttgccacg
    89101 ccaaacggcg cgcggcgggt gatccagtgc agattggtcg agcaatacgc cattacatag
    89161 cgcccatccg aaagcagcat gttgaaaacg cccttctgcc gcagttcatc cgccagtgag
    89221 gcgatatatt taaataccgc cgccatattg cccggcgtac gcggataacg ctgcgttaat
    89281 ttatacagca gccagcaaaa ggctttttcg ctgtcggttt tacctaccgg gcggaagttg
    89341 ccggtttcca gtgatttata gcccgtcagt tgtccgttat gggcataagt ccagttacgt
    89401 ccccataact cgcgggtaaa cgggtgagta ttttccaacg ccacctcgcc ccgatttgcc
    89461 tggcgaatat gagccacaac cgaacaggat tttatcgggt agtcctgaac aagtttggca
    89521 atgggggaat taaagctggg ttgtggatct ttaaatgtgc gacagccttt accttcgtaa
    89581 aaggtaatgc cccagccatc tttatgtggc ccggttccac caccacgctg caccagcccg
    89641 gtgaaactaa agcaaatatc ggtagggacg ttggcgctca tcccgagcaa ttcgcacatc
    89701 ggtaaccccc acaacagcgg gagcgcatgg cccccggcga agattgactt aagaattttc
    89761 aggccggatg cggcgtaaac gcattattcg gcctacgcca gacttactta accatctctt
    89821 tttcaattaa ctggatcagg atatggatca ctttaatgtg aatttcctga atgcggtcgg
    89881 cataaccaaa atgtggaaca cgaatttcaa tatccgccgt accagccatt ttgccgccat
    89941 ctttgccggt cagggtaatc actttcattc ccttctcacg cgccgccgcg atcgctttga
    90001 tcacgtttgc agagttacca gacgtggaga tccccagcag tacatcgcct tcgcggccta
    90061 ccgcttcaac atagcgggag aaaatatcat tgaaaccaaa atcgttaccg acgcaggaaa
    90121 tatggctgac gtcagagatg gcaatcgccg ggtagcccgg acggttttca cggtagcgac
    90181 cggtcagctc ttcggcaaag tgcatggcgt cgcaatggga gccgccgttg ccgcaggaaa
    90241 gcaccttgcc accggcttta aagctgtctg ctaacaggac cgccgctcgc tgaatggcgt
    90301 gaatattggc gtcatctttt aaaaagttag ccagcgtttc cgccgcttcg ttcagttcgt
    90361 tacgaataag atcctggtac atgaggatat ccttcagcat aaatgtaata gacaaaatgc
    90421 agtgtaccgg ataccgccaa aagcgagaag tacgggcagg tgctatgacc aggacttttt
    90481 gccctgaagt gtagataaaa acagcaacaa tgtgagcttc gttgtaatta tattgtaaac
    90541 acattgctaa atgtttttac atccactaca accatatcat cacaagtggt cagacctcct
    90601 acaagtaagg ggcttttcgt tatgatgatt ttgagtattc tcgctacagt tgtcctgctc
    90661 ggcgcgttgt tctatcaccg cgtgagctta tttatcagca gtctgatttt gctcgcctgg
    90721 acagctgccc tcggcgtcgc tggtctgtgg tcggcgtggg tactggtgcc tctggccatt
    90781 atcctcgtgc cgtttaactt tgcacctatg cgtaagtcga tgatttccgc gccggtattt
    90841 cgcggtttcc gtaaggtgat gccgccgatg tcgcgcactg agaaagaagc gattgatgcg
    90901 ggtaccacct ggtgggaagg cgacttgttc cagggcaagc cggactggaa aaagctgcat
    90961 aactatccgc agccgcgcct gaccgcagaa gagcaagcgt ttctcgacgg cccggtagaa
    91021 gaagcctgcc ggatggcgaa tgatttccag atcacccatg aactggcgga tctgccgccg
    91081 gagttgtggg cgtatctgaa agagcatcgc ttcttcgcga tgatcatcaa aaaagagtac
    91141 ggcggcctgg agttttcagc ttatgcccag tctcgtgtgc tgcaaaaact ctccggcgtc
    91201 agcgggatcc tggcgattac cgtcggcgtg ccgaactcat taggccccgg cgagctgctg
    91261 caacattacg gcactgacga gcagaaagat cactatctgc cgcgtctggc gcgtggtcag
    91321 gagatccctt gctttgcact gaccagcccg gaagcgggtt ccgatgcggg cgcgattccg
    91381 gacaccggga ttgtctgcat gggcgaatgg cagggccagc aggtgctggg gatgcgtctg
    91441 acctggaaca aacgctacat tacgctggca ccgattgcga ctgtgcttgg gctggcgttt
    91501 aaactctccg acccggagaa attactcggc ggtgcagaag atttaggtat tacctgtgcg
    91561 ctgatcccga ccaccacgcc gggcgtggaa attggtcgtc gccacttccc gctgaatgtg
    91621 ccgttccaga acggaccgac gctcggtaaa gatgtcttcg taccgatcga ctacattatc
    91681 ggcgggccga aaatggccgg gcaaggctgg cggatgctgg tggagtgcct ctcggtaggc
    91741 cgcggcatca ccctgccttc caactcaacc ggcggcgtga aatcggtagc gctggcaacg
    91801 ggcgcgtatg ctcacattcg ccgtcagttc aaaatctcta tcggtaagat ggaagggatt
    91861 gaagagccgc tggcgcgtat tgccgggaac gcttatgtaa tggacgctgc ggcatcgctg
    91921 attacctacg gcattatgct cggcgaaaaa cctgccgtgc tgtcggctat cgttaagtat
    91981 cactgtaccc accgcgggca gcagtcgatt attgatgcga tggatattac cggcggtaaa
    92041 ggcattatgc tcgggcaaag caacttcctg gcgcgtgctt accagggcgc accgattgcc
    92101 atcaccgttg aaggggctaa cattctgacc cgcagcatga tgatcttcgg acaaggagcg
    92161 attcgttgcc atccgtacgt gctggaagag atggaagcgg cgaagaacaa tgacgtcaac
    92221 gtgttcgata aactgttgtt caaacatatc ggtcacgtcg gtagcaacaa agttcgcagc
    92281 ttctggctgg gcctgacgcg cggtttaacc agcagcacgc caaccggcga tgccactaaa
    92341 cgctactatc agcacctgaa ccgcctgagc gccaacctcg ccctgctttc tgatgtctcg
    92401 atggcagtgc tgggcggcag cctgaaacgt cgtgagcgta tctcggcacg tctgggtgat
    92461 attttaagcc agctttacct cgcctctgcc gttctgaagc gttatgacga cgaaggccgt
    92521 aatgaagccg atctgccgct ggtgcactgg ggcgtacagg atgcgctgta tcaggcagaa
    92581 caggcgatgg acgatttact gcaaaacttc ccgaaccgcg tggttgccgg gctgctgaat
    92641 gtggtgatct tcccgaccgg acgtcattat ctggcaccgt ctgacaagct ggatcataaa
    92701 gtggcgaaga ttttacaagt gccgaacgcc actcgctcac gtattggtcg cggtcagtac
    92761 ctgacgccaa gcgagcataa cccggttggt ttactggaag aggcgttggt ggatgtgatt
    92821 gccgccgacc cgattcatca gcggatctgt aaagagctcg gtaaaaatct gccatttacc
    92881 cgtctggatg aactggcgca caacgcgctg gcgaaggggc tgattgataa agatgaagcc
    92941 gctattctgg tgaaagcgga agaaagccgt ctgcgcagta ttaacgttga tgactttgat
    93001 ccggaagagc tggcgacgaa gccggtaaag ttgccggaga aagtgcggaa agttgaagcc
    93061 gcgtaattga ataacggagc cgaaaggctc cgtttcttta tccgctaatt atttaaaatt
    93121 aaagccatcc ggatggtttt ccaggctgcc ggtcaacgcc gcgaacagca ccgttttacc
    93181 atcaatcgaa agcgcatcgt tcacattcaa ccaggtcagt ttctcttgcg acgttttctc
    93241 atcaatagtc gagaacagcc ccgtcatctg attagatttc tcggaccaca tcacagcgat
    93301 acgttgcgag ccacagtcat gcggtttgca cgcgctcatc acctgatacg tctcatctcc
    93361 caacgttacg gtttgtgcag gggtataagt accgcctttc atcacccagg caggcagctt
    93421 atgcccttgc accatctgat taaatgcagc tttggtggtt tcgccctttg caaggctgct
    93481 aatggttaaa tcatcctgcg ccattgcact ggtggcgatg accagtgcgg cgactgtcgt
    93541 tattgcctta aacatcattc ctcccgagct tatcctgccc atgttattaa cctcctaaag
    93601 cctggcgata acacgataat ttgcaaatca gatatttcca atatcagcat ttgttatcaa
    93661 tttattctgc gctaaggcaa ctgcctgata aggtgttaaa gttataaaac ccttcgcata
    93721 aaggagttgt acaccgtgcc tggtttgaag attacgcttt tgcagcaacc actggtgtgg
    93781 atggatggtc ctgccaacct gcgtcatttt gatcgtcaac tggaaggtat taccgggcgc
    93841 gatgtgatcg ttctaccgga gatgtttacc agcggctttg ccatggaagc ggctgcttcg
    93901 tcgctagcac aagatgacgt agtgaactgg atgacagcca aagcgcagca gtgcaatgcg
    93961 ctgattgcag gcagtgttgc attacaaacg gagtctggtt cggttaaccg ctttttgctg
    94021 gttgagccgg gcggcacggt acatttttat gataagcgtc atctgttccg catggcagat
    94081 gagcatctgc attataaagc gggcaatgcg cgagtgattg tggaatggcg cggctggcgt
    94141 attttgccgt tggtgtgcta cgacttacgt tttccggtgt ggtcgcgcaa tctcaacgat
    94201 tatgacctcg ccatctacgt tgccaactgg cctgctccgc gctctctgca ctggcaggca
    94261 ttgctgacgg cccgcgcgat tgagaatcag gcgtatgtgg cgggatgcaa tcgcgtcggc
    94321 agcgatggca acggctgcca ttatcgcggt gacagccggg tgattaatcc acaaggcgag
    94381 attatcgcta ctgccgacgc gcatcaggca acgcgcattg atgcggagct gtcgatggtg
    94441 gcattgcggg aatatagaga aaagtttccg gcatggcggg atgcggatga gtttaggttg
    94501 cggtgagtgg gggctgtatt tagggcgggg aattcagaaa gggatgttta gtgtgctgag
    94561 cgagagttaa ataataaaaa actgggaaga tttaataatc cccccgcagg gcaagagcat
    94621 gaattaaatc tgtgttaaat ttttcatttt gtcagttcga aacccaagct ctgagcagga
    94681 tgcaagcact tcttctatat aacatgtgtt taacgcaacc ttctttcttt tcctcttcac
    94741 cccaccctta atgtctttgc aatctcttaa taaattcagt gccatcttct ttattccaga
    94801 tattatttcg gctgcgtttc ctcttcttat ccggctggca tcttcattca tttttacatc
    94861 taacacccaa tgaagactgt gctcgatcag ccagtgcgct ctgatagcat gtgcaaattc
    94921 tttagcatcc atatcctttg atgaaatata atatcggatg cttacacctt ctgctgattt
    94981 atcttctttc ttctgcctga atgacaatgc tacacaaagc ttttttaatc ccttccattc
    95041 gaattcaaaa tcacaaaatt caggcgttac gttactgaca atatgcaaac gtgtttcttt
    95101 tcttccatga cttatctcct gcgtactaaa cgaatcgcct ttataattag aaaacacatt
    95161 tacaggaaat ttttcctcga atgcatgatg taatttcccc tgattgcctt ttactgccag
    95221 aagataatct gcttttttat ctttgatctt cgaagcgata tctttctgac agcccatagc
    95281 atcaatggtt atcaaatttt tctttaaatc cagtaggtta agcaactctg gaatggctgt
    95341 aatctcatta cttttggctt ccgtttttac ctgccccagt acaacaccat tttcgttcga
    95401 gaatgcactc accatatgga ttgctccttt tctttttccc ttatcaaagg agcctcttat
    95461 ggtctttcca tctattgcta taatttcacc atcagtgatt tcatggcact cctgcatcca
    95521 ttcaataaat atcttttcaa aggccaaact gtcaatgtta ctcacaacgc gtgcaatggt
    95581 gtcatcgacc ggaatgccat tatcaaaatc accatatttc tttagccatt caagtctttc
    95641 atgtccaaaa tcttcaattt cctgccactc atcggcacct gcaattactg cacatacggt
    95701 gagaaacaaa atatcagata atttatgttt aacctttcct tgttgtcgta tatcagggat
    95761 cactgaaata taatcaagca aactttgaat actcacctgg cgtccttttt atctttttag
    95821 ttattccaaa ggttaaattt tgatcacctg taggcgttaa agccaggatg aaaatcgtga
    95881 tttacatcac gattttcatg atcttgccct gccccgtccc ttgctctcat gtctttctgt
    95941 atgtatttta tatttttctc tttctggtgt tgttcattat gaccatttac caatccagtc
    96001 aaaaacttca tttttcaaat tgttatcata ataatcaaca tcataatagt ctccataatt
    96061 aattatttta taactagcct tcttgttttt atcactaaaa tacacccgtt gttttgtagc
    96121 gcagaactcc atattctcca gtttactaat gttttttaat cgcacgctat aaagaatatc
    96181 attgaaaaaa ttaagattta tatcaaccac tcca
//
LOCUS       DAZFBR010000004        95589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFBR010000004 DAZFBR010000000
VERSION     DAZFBR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.004"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            60..791
                     /locus_tag="ACHJZ2_000340"
     CDS             60..791
                     /locus_tag="ACHJZ2_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4566523.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            927..1847
                     /gene="speB"
                     /locus_tag="ACHJZ2_000341"
     CDS             927..1847
                     /gene="speB"
                     /locus_tag="ACHJZ2_000341"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO4566524.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(2053..2811)
                     /gene="loiP"
                     /locus_tag="ACHJZ2_000342"
                     /gene_synonym="yggG"
     CDS             complement(2053..2811)
                     /gene="loiP"
                     /locus_tag="ACHJZ2_000342"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO4566525.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            3089..5080
                     /gene="tkt"
                     /locus_tag="ACHJZ2_000343"
     CDS             3089..5080
                     /gene="tkt"
                     /locus_tag="ACHJZ2_000343"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4566526.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            5394..5837
                     /gene="cmtB"
                     /locus_tag="ACHJZ2_000344"
     CDS             5394..5837
                     /gene="cmtB"
                     /locus_tag="ACHJZ2_000344"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO4566527.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            5865..7253
                     /gene="cmtA"
                     /locus_tag="ACHJZ2_000345"
     CDS             5865..7253
                     /gene="cmtA"
                     /locus_tag="ACHJZ2_000345"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO4566528.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            7268..8545
                     /gene="yggP"
                     /locus_tag="ACHJZ2_000346"
     CDS             7268..8545
                     /gene="yggP"
                     /locus_tag="ACHJZ2_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4566529.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            8542..9507
                     /gene="glpX"
                     /locus_tag="ACHJZ2_000347"
     CDS             8542..9507
                     /gene="glpX"
                     /locus_tag="ACHJZ2_000347"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO4566530.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            9529..10038
                     /gene="fumE"
                     /locus_tag="ACHJZ2_000348"
     CDS             9529..10038
                     /gene="fumE"
                     /locus_tag="ACHJZ2_000348"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO4566531.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            10035..10748
                     /locus_tag="ACHJZ2_000349"
     CDS             10035..10748
                     /locus_tag="ACHJZ2_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO4566532.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10720..11397)
                     /locus_tag="ACHJZ2_000350"
     CDS             complement(10720..11397)
                     /locus_tag="ACHJZ2_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4566533.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(11391..12068)
                     /locus_tag="ACHJZ2_000351"
     CDS             complement(11391..12068)
                     /locus_tag="ACHJZ2_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4566534.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(12056..12763)
                     /locus_tag="ACHJZ2_000352"
     CDS             complement(12056..12763)
                     /locus_tag="ACHJZ2_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO4566535.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(12764..13342)
                     /locus_tag="ACHJZ2_000353"
     CDS             complement(12764..13342)
                     /locus_tag="ACHJZ2_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO4566536.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(13365..13796)
                     /locus_tag="ACHJZ2_000354"
     CDS             complement(13365..13796)
                     /locus_tag="ACHJZ2_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO4566537.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            14168..15187
                     /gene="epd"
                     /locus_tag="ACHJZ2_000355"
     CDS             14168..15187
                     /gene="epd"
                     /locus_tag="ACHJZ2_000355"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO4566538.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            15237..16400
                     /gene="pgk"
                     /locus_tag="ACHJZ2_000356"
     CDS             15237..16400
                     /gene="pgk"
                     /locus_tag="ACHJZ2_000356"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO4566539.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            16615..17694
                     /gene="fbaA"
                     /locus_tag="ACHJZ2_000357"
     CDS             16615..17694
                     /gene="fbaA"
                     /locus_tag="ACHJZ2_000357"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO4566540.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(17758..17856)
                     /locus_tag="ACHJZ2_000358"
                     /pseudo
     CDS             complement(17758..17856)
                     /locus_tag="ACHJZ2_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18052..18912
                     /gene="mscS"
                     /locus_tag="ACHJZ2_000359"
     CDS             18052..18912
                     /gene="mscS"
                     /locus_tag="ACHJZ2_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO4566541.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            19051..19686
                     /gene="argO"
                     /locus_tag="ACHJZ2_000360"
     CDS             19051..19686
                     /gene="argO"
                     /locus_tag="ACHJZ2_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO4566542.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            19779..20519
                     /gene="yggE"
                     /locus_tag="ACHJZ2_000361"
     CDS             19779..20519
                     /gene="yggE"
                     /locus_tag="ACHJZ2_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO4566543.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            20686..21582
                     /gene="ygfI"
                     /locus_tag="ACHJZ2_000362"
                     /pseudo
     CDS             20686..21582
                     /gene="ygfI"
                     /locus_tag="ACHJZ2_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(21579..23085)
                     /gene="scpC"
                     /locus_tag="ACHJZ2_000363"
                     /pseudo
     CDS             complement(21579..23085)
                     /gene="scpC"
                     /locus_tag="ACHJZ2_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(23109..23894)
                     /gene="scpB"
                     /locus_tag="ACHJZ2_000364"
     CDS             complement(23109..23894)
                     /gene="scpB"
                     /locus_tag="ACHJZ2_000364"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO4566544.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(23905..24900)
                     /gene="meaB"
                     /locus_tag="ACHJZ2_000365"
     CDS             complement(23905..24900)
                     /gene="meaB"
                     /locus_tag="ACHJZ2_000365"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO4566545.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(24893..27037)
                     /gene="scpA"
                     /locus_tag="ACHJZ2_000366"
     CDS             complement(24893..27037)
                     /gene="scpA"
                     /locus_tag="ACHJZ2_000366"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO4566546.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(27241..28134)
                     /gene="argP"
                     /locus_tag="ACHJZ2_000367"
     CDS             complement(27241..28134)
                     /gene="argP"
                     /locus_tag="ACHJZ2_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO4566547.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            28517..29176
                     /gene="rpiA"
                     /locus_tag="ACHJZ2_000368"
     CDS             28517..29176
                     /gene="rpiA"
                     /locus_tag="ACHJZ2_000368"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO4566548.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29431..30663
                     /gene="serA"
                     /locus_tag="ACHJZ2_000369"
     CDS             29431..30663
                     /gene="serA"
                     /locus_tag="ACHJZ2_000369"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO4566549.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            30892..30951
                     /gene="ibsC"
                     /locus_tag="ACHJZ2_000370"
     CDS             30892..30951
                     /gene="ibsC"
                     /locus_tag="ACHJZ2_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO4566550.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            31285..31344
                     /locus_tag="ACHJZ2_000371"
     CDS             31285..31344
                     /locus_tag="ACHJZ2_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4566551.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(31445..32047)
                     /gene="fau"
                     /locus_tag="ACHJZ2_000372"
     CDS             complement(31445..32047)
                     /gene="fau"
                     /locus_tag="ACHJZ2_000372"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO4566552.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(32068..32251)
                     /gene="ssrS"
                     /locus_tag="ACHJZ2_000373"
     ncRNA           complement(32068..32251)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHJZ2_000373"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(32293..32622)
                     /gene="zapA"
                     /locus_tag="ACHJZ2_000374"
     CDS             complement(32293..32622)
                     /gene="zapA"
                     /locus_tag="ACHJZ2_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4566553.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            32790..33368
                     /gene="ygfB"
                     /locus_tag="ACHJZ2_000375"
     CDS             32790..33368
                     /gene="ygfB"
                     /locus_tag="ACHJZ2_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO4566554.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            33394..34719
                     /gene="pepP"
                     /locus_tag="ACHJZ2_000376"
     CDS             33394..34719
                     /gene="pepP"
                     /locus_tag="ACHJZ2_000376"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO4566555.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGKIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            34716..35894
                     /gene="ubiH"
                     /locus_tag="ACHJZ2_000377"
                     /gene_synonym="visB"
     CDS             34716..35894
                     /gene="ubiH"
                     /locus_tag="ACHJZ2_000377"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO4566556.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            35918..37120
                     /gene="ubiI"
                     /locus_tag="ACHJZ2_000378"
                     /gene_synonym="visC"
     CDS             35918..37120
                     /gene="ubiI"
                     /locus_tag="ACHJZ2_000378"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO4566557.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(37194..37277)
                     /locus_tag="ACHJZ2_000379"
     CDS             complement(37194..37277)
                     /locus_tag="ACHJZ2_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566558.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            37568..38662
                     /gene="gcvT"
                     /locus_tag="ACHJZ2_000380"
     CDS             37568..38662
                     /gene="gcvT"
                     /locus_tag="ACHJZ2_000380"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO4566559.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            38686..39075
                     /gene="gcvH"
                     /locus_tag="ACHJZ2_000381"
     CDS             38686..39075
                     /gene="gcvH"
                     /locus_tag="ACHJZ2_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO4566560.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            39193..42066
                     /gene="gcvP"
                     /locus_tag="ACHJZ2_000382"
     CDS             39193..42066
                     /gene="gcvP"
                     /locus_tag="ACHJZ2_000382"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO4566561.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(42232..43665)
                     /gene="bglA"
                     /locus_tag="ACHJZ2_000383"
     CDS             complement(42232..43665)
                     /gene="bglA"
                     /locus_tag="ACHJZ2_000383"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO4566562.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            43710..44021
                     /gene="yqfB"
                     /locus_tag="ACHJZ2_000384"
     CDS             43710..44021
                     /gene="yqfB"
                     /locus_tag="ACHJZ2_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO4566563.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            44185..44844
                     /gene="yqfA"
                     /locus_tag="ACHJZ2_000385"
     CDS             44185..44844
                     /gene="yqfA"
                     /locus_tag="ACHJZ2_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO4566564.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(44921..45901)
                     /gene="ygfZ"
                     /locus_tag="ACHJZ2_000386"
     CDS             complement(44921..45901)
                     /gene="ygfZ"
                     /locus_tag="ACHJZ2_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO4566565.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            46144..46410
                     /gene="sdhE"
                     /locus_tag="ACHJZ2_000387"
     CDS             46144..46410
                     /gene="sdhE"
                     /locus_tag="ACHJZ2_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO4566566.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            46391..46798
                     /gene="ygfX"
                     /locus_tag="ACHJZ2_000388"
     CDS             46391..46798
                     /gene="ygfX"
                     /locus_tag="ACHJZ2_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO4566567.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(46838..47359)
                     /gene="fldB"
                     /locus_tag="ACHJZ2_000389"
     CDS             complement(46838..47359)
                     /gene="fldB"
                     /locus_tag="ACHJZ2_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO4566568.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            47471..48367
                     /gene="xerD"
                     /locus_tag="ACHJZ2_000390"
     CDS             47471..48367
                     /gene="xerD"
                     /locus_tag="ACHJZ2_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO4566569.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            48392..49102
                     /gene="dsbC"
                     /locus_tag="ACHJZ2_000391"
     CDS             48392..49102
                     /gene="dsbC"
                     /locus_tag="ACHJZ2_000391"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO4566570.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            49108..50841
                     /gene="recJ"
                     /locus_tag="ACHJZ2_000392"
     CDS             49108..50841
                     /gene="recJ"
                     /locus_tag="ACHJZ2_000392"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO4566571.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            50932..52030
                     /gene="prfB"
                     /locus_tag="ACHJZ2_000393"
     CDS             join(50932..51006,51008..52030)
                     /gene="prfB"
                     /locus_tag="ACHJZ2_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO4566572.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            52040..53557
                     /gene="lysS"
                     /locus_tag="ACHJZ2_000394"
     CDS             52040..53557
                     /gene="lysS"
                     /locus_tag="ACHJZ2_000394"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4566573.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQLLSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(53600..54148)
                     /gene="idi"
                     /locus_tag="ACHJZ2_000395"
     CDS             complement(53600..54148)
                     /gene="idi"
                     /locus_tag="ACHJZ2_000395"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO4566574.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            54203..54274
                     /locus_tag="ACHJZ2_000396"
     CDS             54203..54274
                     /locus_tag="ACHJZ2_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566575.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(54271..54396)
                     /gene="yqfG"
                     /locus_tag="ACHJZ2_000397"
     CDS             complement(54271..54396)
                     /gene="yqfG"
                     /locus_tag="ACHJZ2_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO4566576.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(54398..55846)
                     /gene="uacT"
                     /locus_tag="ACHJZ2_000398"
     CDS             complement(54398..55846)
                     /gene="uacT"
                     /locus_tag="ACHJZ2_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO4566577.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAQLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            56282..58201
                     /gene="ygfT"
                     /locus_tag="ACHJZ2_000399"
     CDS             56282..58201
                     /gene="ygfT"
                     /locus_tag="ACHJZ2_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO4566578.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            58201..58689
                     /gene="ygfS"
                     /locus_tag="ACHJZ2_000400"
                     /pseudo
     CDS             58201..58689
                     /gene="ygfS"
                     /locus_tag="ACHJZ2_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(58725..60092)
                     /gene="ghxQ"
                     /locus_tag="ACHJZ2_000401"
                     /gene_synonym="ygfQ"
     CDS             complement(58725..60092)
                     /gene="ghxQ"
                     /locus_tag="ACHJZ2_000401"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO4566579.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(60128..61444)
                     /gene="guaD"
                     /locus_tag="ACHJZ2_000402"
     CDS             complement(60128..61444)
                     /gene="guaD"
                     /locus_tag="ACHJZ2_000402"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO4566580.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(61462..62862)
                     /gene="xanQ"
                     /locus_tag="ACHJZ2_000403"
     CDS             complement(61462..62862)
                     /gene="xanQ"
                     /locus_tag="ACHJZ2_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO4566581.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(63027..65897)
                     /gene="xdhD"
                     /locus_tag="ACHJZ2_000404"
     CDS             complement(63027..65897)
                     /gene="xdhD"
                     /locus_tag="ACHJZ2_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO4566582.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(65894..66673)
                     /gene="ygfM"
                     /locus_tag="ACHJZ2_000405"
     CDS             complement(65894..66673)
                     /gene="ygfM"
                     /locus_tag="ACHJZ2_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO4566583.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(66723..68051)
                     /gene="ssnA"
                     /locus_tag="ACHJZ2_000406"
     CDS             complement(66723..68051)
                     /gene="ssnA"
                     /locus_tag="ACHJZ2_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO4566584.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPSPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(68054..71152)
                     /gene="ygfK"
                     /locus_tag="ACHJZ2_000407"
     CDS             complement(68054..71152)
                     /gene="ygfK"
                     /locus_tag="ACHJZ2_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO4566585.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(71474..72052)
                     /gene="mocA"
                     /locus_tag="ACHJZ2_000408"
     CDS             complement(71474..72052)
                     /gene="mocA"
                     /locus_tag="ACHJZ2_000408"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO4566586.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            72155..72925
                     /gene="yqeC"
                     /locus_tag="ACHJZ2_000409"
     CDS             72155..72925
                     /gene="yqeC"
                     /locus_tag="ACHJZ2_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO4566587.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            72973..74598
                     /gene="yqeB"
                     /locus_tag="ACHJZ2_000410"
     CDS             72973..74598
                     /gene="yqeB"
                     /locus_tag="ACHJZ2_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO4566588.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(74639..75571)
                     /gene="arcC"
                     /locus_tag="ACHJZ2_000411"
     CDS             complement(74639..75571)
                     /gene="arcC"
                     /locus_tag="ACHJZ2_000411"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4566589.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(75619..77004)
                     /gene="hyuA"
                     /locus_tag="ACHJZ2_000412"
     CDS             complement(75619..77004)
                     /gene="hyuA"
                     /locus_tag="ACHJZ2_000412"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO4566590.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(77057..78268)
                     /gene="ygeY"
                     /locus_tag="ACHJZ2_000413"
     CDS             complement(77057..78268)
                     /gene="ygeY"
                     /locus_tag="ACHJZ2_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO4566591.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(78326..79522)
                     /gene="dpaL"
                     /locus_tag="ACHJZ2_000414"
     CDS             complement(78326..79522)
                     /gene="dpaL"
                     /locus_tag="ACHJZ2_000414"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO4566592.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(79580..80767)
                     /gene="ygeW"
                     /locus_tag="ACHJZ2_000415"
     CDS             complement(79580..80767)
                     /gene="ygeW"
                     /locus_tag="ACHJZ2_000415"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO4566593.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            81246..83024
                     /gene="ygeV"
                     /locus_tag="ACHJZ2_000416"
     CDS             81246..83024
                     /gene="ygeV"
                     /locus_tag="ACHJZ2_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO4566594.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(83064..83543)
                     /gene="xdhC"
                     /locus_tag="ACHJZ2_000417"
     CDS             complement(83064..83543)
                     /gene="xdhC"
                     /locus_tag="ACHJZ2_000417"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO4566595.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(83540..84418)
                     /gene="xdhB"
                     /locus_tag="ACHJZ2_000418"
     CDS             complement(83540..84418)
                     /gene="xdhB"
                     /locus_tag="ACHJZ2_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO4566596.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(84429..86726)
                     /gene="xdhA"
                     /locus_tag="ACHJZ2_000419"
     CDS             complement(84429..86726)
                     /gene="xdhA"
                     /locus_tag="ACHJZ2_000419"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO4566597.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            87142..87897
                     /gene="actS"
                     /locus_tag="ACHJZ2_000420"
     CDS             87142..87897
                     /gene="actS"
                     /locus_tag="ACHJZ2_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO4566598.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            87976..88049
                     /locus_tag="ACHJZ2_000421"
     tRNA            87976..88049
                     /locus_tag="ACHJZ2_000421"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88008..88010,aa:Gly,seq:ccc)
     gene            88283..89888
                     /locus_tag="ACHJZ2_000422"
                     /pseudo
     CDS             88283..89888
                     /locus_tag="ACHJZ2_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
     gene            89955..90134
                     /locus_tag="ACHJZ2_000423"
     CDS             89955..90134
                     /locus_tag="ACHJZ2_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFO4566599.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
     gene            90480..91229
                     /locus_tag="ACHJZ2_000424"
     CDS             90480..91229
                     /locus_tag="ACHJZ2_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4566600.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
     gene            91226..92929
                     /gene="sctC"
                     /locus_tag="ACHJZ2_000425"
     CDS             91226..92929
                     /gene="sctC"
                     /locus_tag="ACHJZ2_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFO4566601.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGDGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            92934..94079
                     /locus_tag="ACHJZ2_000426"
     CDS             92934..94079
                     /locus_tag="ACHJZ2_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN/LcrE/InvE/MxiC type III secretion system
                     gatekeeper"
                     /protein_id="HFO4566602.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMRDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQSAEALFLKTLMNNPIIKKFIS
                     AEDYWIFFLISLIKFPETAEELLNNALVTLPADANYKDKTLLLKAIYSGCTNLPFSLF
                     INNEQLLEIRECCKQAIKVTFAAELFDTQNCNKKQNKKPWKKVMFNV"
     gene            94072..>95589
                     /locus_tag="ACHJZ2_000427"
     CDS             94072..>95589
                     /locus_tag="ACHJZ2_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4566603.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIVVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLAHNINEYFGIQETKYI"
BASE COUNT        23710 a        23246 c        24882 g        23751 t
ORIGIN      
        1 tttcttatta aataatctcc ctacaatcgc tgcattaatt aaatgcagag ggaacggtta
       61 tgaaaaaatg gaaagtacgc agcgcgttgg tggcattaat cgtgctgctg gccggttgtt
      121 ccagtaatgc gcagtataat agcagtgcat cgggcaatgt cggcaccgcc tggggtgggg
      181 atgtgcactc aacggttcag ggcgtatctg ctgagcgagc ctggcgcgat ccggcggaga
      241 tgatcgttat tagttattcg actaatgttc cttccggcta cgatcgcgtt tatagcattc
      301 gtattaacga actggaatat gctattcgcg atggtaattt taattctcta cctattacgc
      361 gtgtttatga ttcatctaat aatgaaccac gctatattgt ccatgccaga gtcggaatga
      421 attatcaatt atatgtccgt aattacagtc gtaacaccaa ctacgagatc gttgctacgg
      481 ttgatggcct ggatgtgctg aacggcaagc aaggttcgct gaataacaac ggctatattg
      541 tgaatgccgg agattcgtta gcgattaaag gattccgcaa agataaacat accgaagcgg
      601 cttttcagtt cgccaacgtt gctgattcct acgcagccaa ttcggcgcaa ggcgatgtgc
      661 gtaataccgg tgtaatcggc tttgccgcct ttgaattaca aggccaggcg caaaacgcgc
      721 tgccaccgtg ctctggacaa gcctttccgg cagataacaa tggctatgcg ccgccaccgt
      781 gtcggaaata gcaacctcgc ttgatgcctc gcccatttaa ggcgataatt agcacaaact
      841 tgtgtttcgc aaatccaacc cttcctcgtc gggcctaacg acgcggaagg gtttttttat
      901 atcgactttg taataggagt ccatccatga gcaccttagg tcatcaatac gataactcac
      961 tggtttccaa cgcctttggt tttttgcgcc tgccgatgaa cttccagccg tatgacagcg
     1021 atgctgactg ggtgattact ggtgtgccgt tcgatatggc cacttctggt cgtgcggggg
     1081 gacgccacgg tccggcagct atccgtcagg tttcgacgaa tctggcctgg gaacacaacc
     1141 gcttcccgtg gaatttcgac atgcgtgagc gtctgaacgt cgtggactgt ggtgatctgg
     1201 tatatgcttt cggcgatgcc cgtgagatga gcgagaagct gcaggcgcac gccgagaagc
     1261 tgctggctgc cggtaagcgt atgctctcct tcggtggtga ccactttgtt acgctgccgc
     1321 tgctgcgtgc tcatgcgaag catttcggca aaatggcgct ggtacacttt gacgcccaca
     1381 ccgataccta cgcgaacggt tgtgaatttg accacggcac catgttctat accgcgccga
     1441 aagaaggtct gatcgacccg aatcattccg tgcagattgg tattcgtacc gaatttgata
     1501 aagacaacgg ctttaccgtg ctggacgcct gccaggtgaa cgatcgcagc gtggatgacg
     1561 ttatcgccca ggtgaaacaa attgtgggtg atatgccggt ttacctgacc tttgatatcg
     1621 actgcctgga tcctgcattt gcgccgggaa ccggtacgcc ggtaattggc ggtctgacct
     1681 ccgatcgcgc tattaaactg gtacgcggcc tgaaagatct caacatcgtt gggatggacg
     1741 tagtggaagt ggctccggca tacgatcagt cggaaatcac cgctctggca gcggcaacgc
     1801 tggcgctgga aatgctgtat attcaggcgg cgaaaaaggg cgagtaagca ccagatgcga
     1861 tgcgcacggg taaaacgtgc cattaatgtc ggatgcggcg tgaacgcctt atccgaccta
     1921 cgttcggcac ccgtaggccg gataagatgc gccagcatcg catccggcaa tgcgcacaag
     1981 gtaacaaatg tgccattcat gtcagatgcg gcgtgaacgc cttatctgac ctacgttcga
     2041 caccaccagg ctttacttaa tcccatccgc gctcatgcga tcgcgaatat gctgggcgcg
     2101 ttcggcggat gcaggatggt cgtcaaacat tgagctttgg cgaccttctt ccagttttgc
     2161 cagtttttca aagctggtgg caagacctgc cgggctgatg ccgcgttggc gcagaagatc
     2221 gtaagaataa tcatcggctt ctgcttcctg gcgctgggag aattgcgaat taactaattt
     2281 ctcgcccaga tcaccgagtt gtgattgaga taaactgccg acaatcccgc ccgccgaggc
     2341 cgcagctact cgtacggcat ttgtaccaag tgccacctgc attccttttt tcacatggcc
     2401 taatgccacg tgccccattt cgtgaccgat caccgcttcg acttcgttat ccgtcatcat
     2461 atccatcagc ccgctataga cacggataca gccgttagcc attgcaaagg cgttcacatc
     2521 cttcgccata tacactttgt aatttaccgg ctgaccgttg atattgttgc ctagcgcatt
     2581 ggcaatagtt gtcagacgtt tagcgtattc gctattggct ggcgcaatcg tcgccttgct
     2641 gtccatctcc tgacatgcct gatcgctcag ggttttcacc tgcgcatcac tcaaactgta
     2701 agcctgaaaa gcttccgctc ctgatgagag cagtccgttg gagtccatat tctggcaacc
     2761 ggtcagtacc gttgccacgc tcattgctac caataaggcg cgaattttca tgttttttct
     2821 tccgcacaag gtcaggatta ttctaaaaat gcgccgtttg caggtgaacc gacgctcagt
     2881 ctcagtataa ggaaaagcgc agcggacggg cgagtagatt gcgcaacatg cgagcatgat
     2941 ccagagattt ctgaagcggc aaaaggatgt tccatgtaca tgacgcgcgg cttgcggtaa
     3001 attgttggca aattttccgg cgtagcccaa aacgcgctgt cgtcaagtcg ttaagggcgt
     3061 gcccttcatc atccgatctg gagtcaaaat gtcctcacgt aaagagcttg ccaatgctat
     3121 tcgtgcgctg agcatggacg cagtacagaa agccaaatcc ggtcacccgg gtgcccctat
     3181 gggtatggct gacattgccg aagtcctgtg gcgtgatttc ctgaaacata acccgcagaa
     3241 tccgtcctgg gctgaccgtg accgcttcgt gctgtccaac ggccacggct ccatgctgat
     3301 ctacagcctg ctgcacctca ccggttacga tctgccgatg gaagaactga aaaacttccg
     3361 tcagctgcac tctaaaactc cgggccaccc ggaagtgggt tacaccgctg gtgtggaaac
     3421 caccaccggt ccgctggggc agggtattgc caacgcagta ggtatggcga ttgcagaaaa
     3481 aacgctggcg gcgcagttta accgtccggg ccacgacatt gtcgaccact acacctacgc
     3541 cttcatgggc gacggctgca tgatggaagg catctcccac gaagtttgct ctctggcggg
     3601 tacgctgaag ctgggtaaac tgattgcgtt ctacgatgac aacggtattt ctatcgatgg
     3661 tcacgttgaa ggctggttca ccgacgacac cgcaatgcgt ttcgaagctt acggctggca
     3721 cgttattcgc gacatcgacg gtcatgacgc agcatccatc aaacgcgcag tagaagaagc
     3781 gcgcgcagtg actgacaaac cgtccctgct gatgtgcaaa accatcatcg gtttcggttc
     3841 cccgaacaaa gccggtactc acgactccca cggtgcgccg ctgggtgatg cagaaatcgc
     3901 tctgacccgc gaacagctgg gctggaaata cgcaccgttc gaaatcccgt ctgaaatcta
     3961 tgcgcagtgg gatgcgaaag aagcaggcca ggcgaaagaa tctgcatgga atgagaagtt
     4021 tgcggcttac gcgaaagctt atccgcagga agcggctgaa tttacccgcc gtatgaaagg
     4081 cgaaatgccg tctgacttcg acgccaaagc gaaagagttt atcgctaaac tgcaggctaa
     4141 tccggcgaaa atcgccagcc gtaaagcgtc tcagaatgct atcgaagcgt tcggcccgct
     4201 gttgccggaa ttcctcggcg gctccgctga cctggcaccg tctaacctga ccctgtggtc
     4261 tggttctaaa gcaatcaacg aagatgctgc gggtaactac atccactacg gtgttcgcga
     4321 gttcggtatg accgcgattg ctaacggtat ctccctgcac ggtggcttcc tgccgtacac
     4381 ctccaccttc ctgatgtttg tcgaatacgc acgtaacgcc gtacgtatgg ctgcgctgat
     4441 gaaacagcgt caggttatgg tttacaccca cgactccatc ggtctgggcg aagatggccc
     4501 gactcaccag ccggttgagc aggttgcttc tctgcgcgtg accccgaaca tgtctacatg
     4561 gcgtccgtgt gaccaggttg aatccgcggt cgcgtggaaa tacggtgttg agcgtcagga
     4621 cggtccgacc gcgcttatcc tctcccgtca gaacctggcg cagcaggaac gtactgaaga
     4681 gcaactggca aacatcgcgc gcggtggtta tgtgctgaaa gactgtgctg gtcagccgga
     4741 actgatcttc atcgcgaccg gttcggaagt tgaactggct gttgccgcct acgaaaaact
     4801 gactgccgaa ggcgtgaagg cgcgcgtggt ttccatgccg tctaccgatg cattcgacaa
     4861 gcaggatgcg gcttaccgtg aatccgtact gccgaaagcg gttactgcac gcgttgcggt
     4921 agaagcgggt attgctgact actggtacaa gtatgttggc ctgaatggcg ctatcatcgg
     4981 tatgaccacc ttcggtgagt cagctccggc agagttgctg ttcgaagagt tcggcttcac
     5041 cgtcgacaac gtcgttgcga aagcaaaaga actgctgtaa ctagcatttc gggtaaaaag
     5101 gtcgcttcgg cgaccttttt tattaccttg atatgtccgt ttgcggacaa gcaatagata
     5161 aagcgtgttg tagatcgcaa atatttatat gcaataaata tcaattatgt aatatgcatc
     5221 acgatatgcg tgttgacatt tgttgttata tctataactc aatgttatat aagaaattaa
     5281 taattcactg ttttcaaaac accggtttcc ctgctcaatt gctttcatta aaccgcacga
     5341 gtattttatt tttaccgccg gacggtaccc taaaaataaa ataaggataa tttatgcggc
     5401 ttagtgacta ttttccagaa tcatcaatct cagttataca ttcagcaaaa gattggcagg
     5461 aagctatcga tttctcgatg gtatcattgc tggataaaaa ctatatcagc gagaattaca
     5521 ttcaggcaat taaagattcc accattaaca atggccctta ttatattctc gcaccaggcg
     5581 tggcaatgcc tcatgcgcga ccggaatgtg gggcgcttaa aaccgggatg tctttgacat
     5641 tacttgaaca aggtgtttat tttccgggga atgacgaacc aattaaatta ctcatcggac
     5701 tctctgctgc cgatgccgat tcgcacattg gcgctattca ggcgttaagt gaattactgt
     5761 gcgaagaaga aatactcgaa caactcttaa cagcatcatc agaaaaacaa ttagcggaca
     5821 ttatcagccg cggataatta ctctctcctc tacaggataa tattatggaa aacaagtctg
     5881 ctcgtgcaaa ggtccaggct tttgggggct ttttgactgc aatggtcatc cccaatattg
     5941 gtgcttttat tgcctggggt tttattactg cgttatttat tcccaccggt tggctgccta
     6001 acgaacattt cgccaaaatt gtcggcccga tgattaccta tttattgccc gtgatgattg
     6061 gttctaccgg tggtcatctg gtcggcggta aacgcggcgc ggtcatgggc ggaataggta
     6121 ctattggtgt gatcgtcggc gcagagatcc cgatgttcct tggctcaatg attatggggc
     6181 cgctcggtgg tctggtcata aaatatgttg ataaggcact ggaaaaacgc atacctgccg
     6241 gttttgagat ggttatcaat aacttctcat taggtatcgc ggggatgctc ctttgcctgc
     6301 tgggttttga agtgatcggc ccggcggtgt taattgccaa tactttcgtc aaagagtgta
     6361 ttgaggcgct ggtacatgcg ggttatctgc ctctgctgtc agtcatcaat gaaccggcga
     6421 aagtgctttt ccttaataat gcgatcgatc agggcgtcta ttatccgctg ggaatgcaac
     6481 aggcttcggt taacggtaaa tccatcttct ttatggtggc ctctaaccca ggtccgggcc
     6541 tggggctgct gctggcgttt accttgtttg gtaaagggat gagtaaacgt tctgcgcccg
     6601 gggcgatgat tattcacttc ctcggtggga tccacgaact gtatttccca tatgtgctga
     6661 tgaagccgct gactattatt gccatgattg cgggcggtat gtctggcacc tggatgttta
     6721 acttactgga cggtggtctg gtggctggcc caagtccggg gtctatcttt gcttacctgg
     6781 cgctgacgcc gaaaggttcg ttcctggcga caattgccgg tgttacggta ggtaccctgg
     6841 tgtcctttgc tatcacttcg ctgatactga agatggaaaa aacggtggaa acggagagcg
     6901 aagatgagtt tgctcagtca gccaatgcgg ttaaggcgat gaaacaagag ggtgcattct
     6961 cgttaagcag ggttaagcgt atcgcctttg tttgcgatgc ggggatgggc tccagtgcga
     7021 tgggcgcgac caccttccgt aaacgcctgg aaaaagcggg gctggcaatt gaagtaaaac
     7081 attacgccat agaaaacgtg cctgcggatg cggatatcgt cgttactcat gccagtctgg
     7141 aagggcgcgt gaaacgtgtg acggataaac cactgatatt gattaataac tatattggcg
     7201 atccaaaact cgacacttta tttaatcaat taaccgccga acataaacac tgattacaga
     7261 ggtaaaaatg aaaaccaaag ttgctgctat ttatggcaag cgggatgtcc gtctgcgcga
     7321 atttgaactg ccagaaatta ccgataatga attactggtg agtgtgattt ctgacagcgt
     7381 ctgtttatcc acctggaaag cggcgttact cggtagtgaa cataaacgcg tacccgacga
     7441 tttagaaaat catccggtca ttaccgggca tgaatgtgcc ggggttattg tcgaagtggg
     7501 taaaaatctc actggcaaat ataaaaaagg ccagcgtttt gtattgcaac cggcgatggg
     7561 gttaccaagc ggatattcag cgggctacag ctacgaatat tttggcggca atgccactta
     7621 tatgattatt cccgaaatcg ccattaattt gggctgcgta ttaccgtatc acggctctta
     7681 ttttgctgcg gcgtcgctgg cagagcctat gtgctgcatt attggcgctt atcatgccaa
     7741 ttatcacacc acgcaatatg tttatgagca tcgcatgggc gtcaaacctg gcggcaatat
     7801 tgcactgctg gcgtgtgcag gtccgatggg cattggcgct atcgattacg ccattaacgg
     7861 cggcatacaa ccgtcgcggg tggtggtggt cgatatcgac gacaaacgtc tggcgcaggt
     7921 acagaagctg ctgccggtgg aactggcggc cagtaaaggc attgagctgg tgtatgtgaa
     7981 taccaaaggg atgagcgatc ctgtccagat gctgcgggcg ctgacaggag atgccgggtt
     8041 cgatgacatt tttgtttatg cggcggtgtc tgctgtcgtt gagatggctg atgaattact
     8101 ggcggaagat ggctgtctga acttctttgc cgggccgacg gataaaaact tcaaagtgcc
     8161 gtttaatttc tacaacgttc attacaacag cacgcacgtc gtcggtacat ccggcggttc
     8221 aacggacgac atgaaagagg cgattgccct tagcgccaca gggcagttac agccctcgtt
     8281 tatggtgacc catatcggtg gcctggatgc ggtgccagaa accgtgctca atctgccgga
     8341 tatccctggc ggtaaaaaac tcatttataa cggcgtgacc atgccgctca ctgccattgc
     8401 cgattttgcc gaaaaaggca aaaccgatcc gctgtttaaa gagttggcgc ggctggttga
     8461 ggaaacgcac ggcatctgga atgaacaggc cgagaaatat ctgctggcac aatttggcgt
     8521 tgatatcggg gaggccgcgc aatgatgtcc ctggcgtggc cattatttcg cgttacggaa
     8581 caggcagcgt tggctgcctg gccgcaaacc ggatgtggcg acaaaaataa aattgatggc
     8641 ctggcggtca ccgcgatgcg ccaggcatta aacgacgtcg cttttcgtgg acgagtggtt
     8701 atcggcgaag gagaaattga ccatgcgccg atgctgtgga ttggcgaaga ggtgggcaaa
     8761 ggcgatgggc cagaagtcga tatcgcggtt gatcccattg aaggcacgcg gatggtggcg
     8821 atggggcaga gcaatgcgct ggcggtcatg gctttcgccc ctcgagatag cctgttacat
     8881 gcgcccgata tgtatatgaa aaagctggtt gttaatcgac tggctgccgg ggcgattgat
     8941 ctgtctttgc ctctggcgga caacttgcgt gacgtggcgc gggcgttagg taaaccgctg
     9001 gacaagctgc gcatggttac gctggataaa ccgcgtctga gtgccgcgat tgaagaagca
     9061 acacagctgg gcgtgaaggt ttttgccctg ccggatggcg atgtcgccgc cagcgtgctg
     9121 acctgctggc aggataatcc ttacgatgtg atgtacacca tcggcggtgc gccggaaggc
     9181 gtgatttctg cctgtgccgt taaagcatta ggcgggaata tgcaggccga gctgattgac
     9241 ttttgtcagg ccaaagggga ttacacggaa aatcggcaga ttgcagagca ggagcgcaag
     9301 cgttgcaagg caatgggtgt tgacgttaac cgtgtgtact cgctcgatgt actggtaagg
     9361 gggaacgata tcctctttag cgctacgggc gtcacgggcg gcgagttggt gaacggtatc
     9421 caacagacgg cgaatggagt gcggacgcag acattactga tcggcggcgc ggaccaaacg
     9481 tgtaatataa tagactccct gtattgatgg tgatctatcg gaagaccaat ggcgacgctg
     9541 tcagaagatg atgtgcttga gcaactggat gcacaggaca atttattttc atttatgaaa
     9601 actgcgcatt ctattttgct ccaggggata cgccagtttc tgccgtcgct gtttgtcgat
     9661 aacgatgaag agagcgtcga atatgcagtg aagccgttac tcgcccaaag cggcccgctt
     9721 gacgatattg atgttgcact gcgtttgatt tatgcgctgg ggaaaatgga taaatggctg
     9781 tacgccgata tcacgcattt ttcccagtac tggcattacc tgaaagaaca ggatgaaacg
     9841 ctcggatttg ccgatgacat gacttgggat tttatcagca acgtcaatag cataacctgc
     9901 aatgcgacgc tctatgacgc gttgaaagcg atgaagttcg ccgatttcgc cgtctggtca
     9961 gaggcgcgtt ttagcggaat ggtcaaaacg gcgctgacgc tggcagtaac gaccacctta
    10021 aaggaattaa cgccgtgaaa attgaattaa cggtgaatgg gctgaacgtg caagcgcagt
    10081 accatgatga agagatagaa cgcgtgcata aaccgttgct gcgtatgctg gcggccttac
    10141 agacggtaaa tccgcagcgc cgaaccgtgg tttttctttg cgctccgccg gggacgggta
    10201 aatctaccct aaccaccttc tgggcatatc tcgcgcaaca agacccggag ctgcccgcta
    10261 ttcagacgct cccgatggat ggttttcacc attacaatag ctggctggat gcgcatcaat
    10321 tgcgcccctt caaaggcgca ccagagacat ttgacgttgc gaaactggcg gaaaatctgc
    10381 gccaggtcgt ggaaggggat tgtacgtggc cgcagtacga tcgacaaaag catgatcctg
    10441 ttgaagatgc gttgcacgtt accgcaccaa taatcatcgt cgaaggaaac tggctattgc
    10501 tggatgacga taactggcgc cagctagcgc aattttgtga tttctcgatc tttatcaatg
    10561 cgccagctac tgcgttgcgg gaacgactgg tggggcgcaa gctggcggga gggttatcgc
    10621 tggcagacgc agaagctttt tatgaccgta ccgacgggcc gaatgttcgc cgggtgctgg
    10681 aagagagtct gcctgcaaat ttgaccttaa tgatgaccgc tacgggtgaa tatcgtctgg
    10741 tggattaatg ttcctgatta gaccatcttc cagtcgcacc acacgagaga aatgacgccg
    10801 ggtaaatgcg gcgtcatgac tgattgctac gacgctcgtg cctcgctggc ggcacttctc
    10861 cagccagctt tcaaacacgc tcaaccaatt ttcgtcaaaa tcccgactcg gctcgtccag
    10921 cagcaatagt ggcggagaga gtgcttccag gcaggcgacg gcgaccatcc gtcgctgcgc
    10981 actatgcaga tccaacgggt gcgcgctggc agtgtctgct aactgacaac attgcagggc
    11041 agcatgggta cgttgagtga tttcatccgc cgggcatttt tgcagtttca aaccaaatgc
    11101 gatctcgtca gcgacggtgc tgtgaaaaag ttggttttcc gcttcctgaa acagtacgcc
    11161 aatctttgcc gcgcgctggc ggtttttcag gtttttcatc gcctgttgtt gcagcataac
    11221 cgtaccggct gtgggcgtaa gaagacctgc cattactcgt aacagcgtcg atttccctgc
    11281 gccattgtcg ccggtcaacg ccaaccattc accttgtttg agttgcagag aaatgtcgca
    11341 aagacaatcc gttgccgcgc caggccagcg ataagatatc tgatttaacg ttaacatagt
    11401 ggctggagcg ctccgttttg caattgccac gcttgctggc agtagttcag gaaaggtgtg
    11461 cgatgacgtt caaaaagaat aatcagcgaa ccacgttcaa acgcccagtg ttgtaagcgc
    11521 tgcaacagca tttcgctggc ctgcggcgtc aggcggctaa aggcttcatc aagaattaat
    11581 agctttggct gcatcgcgat ggcgcaggcg atgaccacgc gctgtgtctc tccaccggag
    11641 agtgttgccg ggtgacgatg gcgcaacggc tggcactcgg tcagagtcag ggcggcatca
    11701 atccgcgcca taatctctgc ttcagcaaga cacagatttt ctggcccaaa ggcaacttct
    11761 tcttccacgc taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg
    11821 atggtggcgg attgttcatt aagcgggaga tgcccaatgg gcgtaccgag gagcgtcccc
    11881 gtgcctgtta tctctcctgg cagaaagtcc ggataccagc ccgccatcag ctgtgccagc
    11941 gtacttttgc cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga
    12001 aagtcataac agaacggagg atgcgttgag tgtgtcgggc aataacggaa ctgttctaac
    12061 gtaaccatat ccacgctccg aattctgtca gcatcagcaa gatcatggtg taacgcgcta
    12121 cgcgttgtag ggtgctatcg gctggtgccc acaacgttgt tcgtcggttg tggatccgaa
    12181 atgcccgcat atccagcgcc gcaccacgga cagcaagatc gtttagcgca ttgtgtgtta
    12241 gcggaatagt taacgcgggc atcgcacgta aacgctgata ccagccctca tccagcggca
    12301 cgccgcgggc acgctgcgct tcatgaataa tcgctaattg ccgttttaac tgctcaacga
    12361 ccaataacgg cccggcaaac agataagcga cgcccggcgg caggcgagaa gcaaacagag
    12421 cgcggataaa acattgcacg gggacgtact gcatccacaa ctgtgaggtt gaaacaatcg
    12481 ccagaatccg caaccagagc gtaatagcat gcgtccaacg ctccggcgaa cgcggcgtgc
    12541 cgcttagcca ttctgttagc cagccaccgt gtaccagcca cagcccggct cccagcgaga
    12601 acatcagcca gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaaa
    12661 aaaaggtcgc cgcgctatag atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc
    12721 aggccgccaa tgcccatagt gttaatgagg taaatggatg catttagcgc acggcagcca
    12781 ttgccggaaa atggcgcgtg gtgcgaatcg gcagctggcg cagtaaaagc cagacaatca
    12841 ccgctgtaag agttttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca
    12901 ccaggttttg ccccatcgag tgcatccagg caacgaacaa atcggctccg ctaccggtga
    12961 caccgccgaa cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa
    13021 tcacgccgct gacaacgact tttggcaggg tgcgaaacca acccgctcgc gccagccaac
    13081 cggcgaccag gccgatgacc atcgcgacgg gggcaaaagc ggcagcgatc gggtcggtca
    13141 gtaatcccca gagtaaattg gtcagtaagc cggtcagcat cccgatcacc ggaccaagca
    13201 gtaccgcgct aataagtgtg ccgatggagt cgagaaatat aggtaatttg accatgctgg
    13261 ccagttgccc gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac
    13321 tggaaaagtg gctacgcgcc ataagaaaac ctctctgaaa gtagttattt aacgggggaa
    13381 atatgcaact cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca
    13441 aggctaccga tcaccagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg
    13501 ccaagcatcg tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc
    13561 aacgtgccgt ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc
    13621 ccggcatagc gcaaggcgat gtcagtcagg ctaccggtac agcctgcaat ccaggcggca
    13681 tgaagttgct gctgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagg
    13741 aggcgcagtg cgtaaaagcg tgcggaggaa gaatatgggt agggcaatgt catcattgga
    13801 gtactccgaa actcggacgt attacggata atatcgacgc tgcatcagtc atattctgat
    13861 ataagtcagg gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg
    13921 caaagtggaa taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agataattgt
    13981 tttctttgac tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt
    14041 ttcagtcgat taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt
    14101 tccccggacg atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag
    14161 gagatctatg accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt
    14221 tcgtgctttg tatgaatccg gacgccgggc ggaaattacc gtggtggcaa tcaacgaact
    14281 ggcggatgct gcgggcatgg cgcatttgtt gaaatacgac accagccatg gccgttttgc
    14341 atgggaagta cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt
    14401 gcatgaacgt tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga
    14461 ctgcaccggc gtatatggct cccgcgagca tggcgaagcg cacattgccg ccggggctaa
    14521 aaaagtgctc ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt
    14581 caatcaggat caacttcgtg cggagcaccg catcgtttct aacgcttcct gtaccacgaa
    14641 ttgcataatt cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt
    14701 gaccacaatt cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct
    14761 gcgtcgcacc cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgccgg
    14821 tatcacacga ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc
    14881 aaccataaat gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa
    14941 tgaagtcaac ctgttgctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta
    15001 tacggaattg ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga
    15061 tggcacccaa acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa
    15121 cgaatggggc tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt
    15181 caggtaagac gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt
    15241 ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc
    15301 tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc
    15361 cgaccatcga actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc
    15421 gtcctaccga aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga
    15481 aagacaaact gtctaacccg gttcgtctgg ttaaagatta cctcgacggc gttgacgttg
    15541 ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg
    15601 acgaaatcct gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg
    15661 gtactgctca ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt
    15721 gcgcaggccc gctgctggca gctgaactgg acgcgctggg taaggcactg aaagaacctg
    15781 ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg
    15841 actccctgtc taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta
    15901 tcgcggcaca aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag
    15961 caaaacgtct gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg
    16021 agttctctga aaccgctccg gctaccctga aatctgttaa cgacgtgaaa gctgacgagc
    16081 agatcctgga tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga
    16141 aaaccattct gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta
    16201 ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg
    16261 acactctggc agcaatcgac ctgttcggca ttgctgacaa aatctcctac atctccactg
    16321 gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg
    16381 aagagcgcgc taagaagtaa aaaatcacag ggcagggaaa cctgcccttg tttcagcgcg
    16441 cttttagagc acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga
    16501 gagtgaatcc ccaggagctt acataagtaa gtgactgggg tgagcgaacg cagacgcagc
    16561 acatgcaact tgaagtatga cgagtataag gcccgacgat acaggacaag agacatgtct
    16621 aagatttttg atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc
    16681 caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc
    16741 atcaacgccg tactggaaac tgctgctaaa gttaaagcgc cggttatcgt tcagttctcc
    16801 aacggtggtg cttcctttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct
    16861 gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt
    16921 gttccggtta tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt
    16981 ctgctggacg cgggtgaaaa acacttcgca gctaccggta agccgctgtt ctcttctcac
    17041 atgatcgacc tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg
    17101 gagcgcatgt ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa
    17161 gaagacggcg tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac
    17221 gttgattacg catacaccga actgagcaaa atcagcccgc gtttcaccat cgcagcgtcc
    17281 ttcggtaacg tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg
    17341 cgtgattctc aggaatatgt ttctaagaaa cacaacctgc cgcacaacag cctgaacttc
    17401 gtattccacg gtggttccgg ttctactgct caggaaatca aagactccgt aagctacggc
    17461 gtagtgaaaa tgaacatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac
    17521 tactacaaag cgaacgaagc ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat
    17581 cagccgaaca agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg
    17641 atcgctcgtc tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagtcatt
    17701 cctttctgct tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaaattgc
    17761 ctgatgcgct atgcttatca ggcctacacg tatcctgcaa tatattgaat ttgcaggttt
    17821 ttgtaggccg gataaggcgt tcacgccgca tccggcatga caaagcgcac attgatatct
    17881 gtctgagccc cgctctgcgg ggattttttt gccaaaagca aggtgattca gatgagaaat
    17941 ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc ttgtcagact gcccgtcata
    18001 aggcggcgga gtgtatttct ccattttgag tcagttgaaa aggaatattg aatggaagat
    18061 ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc tggtagctaa ccaggcgctg
    18121 ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga tcatcatcgt tggtttgatt
    18181 atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga tctcccgtaa aatcgatgcc
    18241 actgttgctg attttctttc tgcattagtc cgttacggta ttatcgcctt tacgctaatc
    18301 gcagccctgg gacgcgtggg tgtacaaaca gcgtcagtca ttgctgtact cggtgccgca
    18361 ggcttagctg ttggtctggc tttgcagggg tcactttcta acctggccgc tggcgtgtta
    18421 cttgtcatgt tccgcccgtt ccgtgccgga gaatatgttg acctcggcgg cgtagccggt
    18481 actgtgctga gtgtgcagat tttctccacc accatgcgta ctgcagacgg taaaattatc
    18541 gttattccga acggtaaaat tattgccgga aatattatta acttctcccg cgagccagtt
    18601 cgccgtaacg aatttattat tggcgtggcg tatgattccg atatcgatca ggttaagcag
    18661 atcctgacca atattatcca gtctgaagat cgcattttga aagatcgcga aatgactgtg
    18721 cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg tccgcgtctg gagcaacagc
    18781 ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta ttaaacgtga atttgatgcc
    18841 gccggtatca gcttcccgta cccgcaaatg gatgtgaact ttaagcgggt gaaagaagac
    18901 aaagctgcgt aatcaacgct gaggccagat aatactctct ggcctctctc ttattagtta
    18961 ttctgattgc caattaatat tatcaatttc cgctaataac aatcccgcga tatagtctct
    19021 gcatcagata cttaattcgg aatatccaac gtgttttctt attactttca aggtcttgct
    19081 cttggggcag ctatgattct gccgctcggc ccacaaaatg cttttgtgat gaatcagggc
    19141 attcgtcgtc agtaccacat tatgattgcc ttactttgcg ctatcagcga tttggtcctg
    19201 atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc agtcgccgtg gttgctggcg
    19261 ctggtcacct ggggcggcgt agtcttcttg ctgtggtatg gttttggcgc ttttaaaaca
    19321 gcgatgagta gtaatattga gttagctagt gccgaagtcc tgaagcaagg cagatggaaa
    19381 attatcgcca ccatgttggc agtgacctgg ctgaatccgc atgtttacct agatactttt
    19441 gttgtactgg gcagccttgg cgggcagctt gacgtcgaac caaaacgttg gtttgcgctc
    19501 gggacaatta gcgcctcttt cctgtggttc tttggtctgg ctattctcgc agcctggctg
    19561 gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca atctggtagt gggatgtgtc
    19621 atgtggttta tagccttgca gctggcgaga gacggtattg ctcatgcaca agccttgttc
    19681 agttaggcgc tatctgatgg aaaaataaaa cagaggcgct aagcttgcct ccagagatcc
    19741 tgaattttgg gcaagtcaat taacacggag agactaacgt gaagttcaaa gttatcgccc
    19801 tggcggcatt aatgggtatt agcgggatgg cagcgcaggc taacgaattg ccggatggac
    19861 cgcatattgt cacctccggt acggcaagtg tggatgcggt gccagacatt gccactcttg
    19921 cgattgaagt taacgtggcc gcgaaggatg ccgctacagc caagaaacag gcagatgagc
    19981 gcgtcgcaca atacatttcc ttccttgaac tcaatcagat cgcgaaaaaa gatatcagct
    20041 cagcgaattt acgcacccag ccagattatg attatcagga tggtaaaagt atcctgaaag
    20101 gctaccgcgc tgtgagaacg gtggaagtca cgctccgtca gttagacaaa ctgaattcct
    20161 tgctggatgg cgcgctgaag gcgggtctta acgaaattcg ttcagtgtcg ctgggcgtgg
    20221 cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc gattgataac gcgattcatc
    20281 aggcgcagga actggcgaac ggctttcatc gtaaactggg gccggtatat agcgtgcgct
    20341 accatgtttc caactatcag cccagcccaa tggtgcggat gatgaaagcc gatgccgcgc
    20401 cggtgtctgc ccaggaaact tacgagcagg ccgctattca gtttgatgat caggtcgatg
    20461 tggtcttcca gttagaacct gtggatcaac aacccgctaa aacacctgca gcacaataat
    20521 acttgtagcc cgacgtattc gtcgggctaa tgcctttacc cgatattgcc tcctttttct
    20581 gtaattttct gtaaaaccat gacgtaatca cctatctttg ctcgcggttt catcaaggat
    20641 aggctgactg aaacggttaa gcggacaagg atgcggggag gggggatgga catttttatt
    20701 tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca ataatattgc ccgggcggca
    20761 gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca ttgccgcgct ggaagaacaa
    20821 attggctata cgctatttac ccgcaaagat aataatatca gcctcaataa agccggtcag
    20881 gaactgtatc aaaaactgtt cccggtttat caacgacttt ctgcgattga taatgaaatc
    20941 cataactcag ggcgtcgttc acgagaaatt gtgattggca tagataatac ttatccgaca
    21001 attattttcg atcagttgat tagtctcgga gataagtatg aaggcgtgac cgcccagcca
    21061 gttgagttca gcgaaaacgg cgttatcgat aatctttttg accgtcagtt agattttatt
    21121 atctccccac agcatgtatc tgcacgagtt caggaactgg aaaacctgac tatcagtgaa
    21181 ttaccaccgc tgcgattgaa ctttctcgtt tctcgccgtt atgaagagcg tcaagagcaa
    21241 gagctgcttc aggagctgcc ctagctacaa atgcgctttc aaaaccgggc taacttcgaa
    21301 gcaatgattg atgcgaatat gcgaccttgt gggatcaatc ccaccattat ttatcgtccc
    21361 tacagtttta tggccaaaat cagcgccgtg gagcgcggac attttctgac ggtgatcccg
    21421 cattttgcct ggcgactggt gaatccggca acgctgaaat attttgatgc gccgcacagg
    21481 ccgatgtata tgcaggaata tctttattca atcagaaatc atcgttatac cgccacgatg
    21541 cttcagcata ttgctgaaga tcgtgacggg acaaaccatt aacccagcat cgagccggtt
    21601 gcaattaaat tacggtgtaa gtcgaagacg tggctaagat cgtggtgaat atgtccgccg
    21661 ggcgcatttt ccagatagcg atgcagataa tcccgataca taggatgtgc acaattatca
    21721 atgatagtgc gggcgcgttg aagcggggaa agaccgcgca gatcggcgat cccttgttcg
    21781 gtgatgatca ctttgacgct gtgttcgttg tgatcaacat ggctgcacat tggcacgacg
    21841 gttgagatct tgccttcttt agcaatcgac ggagccataa agatcgacag atacgcgttg
    21901 cgttcaaaat caccgctgcc gccgatgccg ttcatcagat cgaccccggc tacgtgcgtt
    21961 gagttggcat gcccgtaaat atcaaactcc aggccgacgt tcagagcgat gacgcccaga
    22021 cgacggatga tttccgggtt attggaaatc tcctgcggac gcaacacaat gcggctggca
    22081 aagtaatcca tattgtcgta aatcttgcgc agggaatcgg ccgagattgt caggctggag
    22141 gcgctggccc cgctgatttt gccggtttcc agtaaatgca ccaccgattc ctgtagcact
    22201 tccgaataca tcataaacgg aggaatttcc gggttttccc ccagacgcgc cattaccgca
    22261 ttattgatat tgcccacgcc actttgcagc ggcagaaatt ccggcggaat acgcccatgc
    22321 gccatttcct gcaataagaa cgtgaccacg ttatcggcaa tctgctggca catgggattt
    22381 tgcttatcca gcatattacc ggcgtcgggc aagttggttt ccacgacggc gacaatcttt
    22441 ttcggatcga tttgcacata gcgggtaccg acgcgatcca ttgcatggaa gatcgacacg
    22501 ctattgcgcc gcggtggcgc gccaggaatc acaatatccg ccagttctgc aacgcgcgga
    22561 tcgtgatagt gattgagttc aatgatcact ttcttcgctc gcagcagcca ggtcggcgca
    22621 ttaccgatcc cgctggttaa ccagactcga ccatctggtg ccagtgccga tgcttcaatg
    22681 acggcaacat caatgtcgcc gaggaaaccg taattgacca tttgcgccac ttcgctcaaa
    22741 tgcaggtcaa cgaaactcac cgcgccctga ttgatctttt tacgtaaacc ggacgatgtt
    22801 tgatatggcg cacgccagga aacagcatcg gcgtcagaaa gtacatcgtc agcggcggcg
    22861 ctgattgacg cccccgtcag cggcggcgct gattgacgcc cccgtcagca ggcgaatttg
    22921 atacggcttt ttggcctcat gctgttcgtt agctctgcgg gcaatcgcgg tgggtagggc
    22981 tttcggcgaa cccgctgggg taaagccgct aaatgccacc atgtcgttat gctggataat
    23041 ttctgccgct tcatcggcgg tcatccttgt ccactgagtt tccattttta ctccttcgtt
    23101 cgcagggatt aatgaccaac gaaattaggt ttacgttttt cgaggaaagc gttcatccct
    23161 tcctggtaat cttcgctgtc ataaaccgcg cggcgcatcc cctgaatacg ttcaaattca
    23221 tcggagttca tggtgtgtgc ttcgcccagt acacgcagct cttctttgat aacggcaatg
    23281 gctaacggcg ctttctcaga gatgtggtgc gccatttgta aggtgaaatc ttccagttct
    23341 tccacttcca caacatggtt gaggatgccg acagccagcg cgcgctgggc ggtgatgggc
    23401 gaagcggtaa aaatcagctc tttgacaatg tggaagcccg cgtcgcgggt caggttgtga
    23461 atgccgacca ggttatacgg gacgccgagg tttacaggcg tcattgagaa ggttgaggta
    23521 ctggcggcga taatcagatc ggaactcatg atcatttcaa atgcgccacc ccaaacacta
    23581 ccttccacca tcgaaataat cggtttcggg aatttttgga tcatgcgggt gatttgacgc
    23641 aatggatcat cataggagag cggatcgcga ccgccagacg gcagttcgtg aatatcgtga
    23701 cctgcggaga agactttgga tccactcggc gcgcgcaaaa tgatacagcg aatttccggc
    23761 cggttgagat cgcttaacgc ctgcataaga tcatcaataa agactttact taaggcatta
    23821 agttttcggc catagttaaa ctcaatgacc gccactttgt tgatagtgac aacgttaaca
    23881 tactgataag acataaaaat tcctttaatc aaaatattgc gtctggataa attcactgag
    23941 ctgccgcagg ccggtgcgcg gtgagagcgt attgtttttg accgctaaaa gcgtctggcg
    24001 gtaatagcga tcgaaatctt cattcgcgaa caggtgattc agtacttctt cttcggtctg
    24061 cttacgcagc cattccaccg attgttgttg ccgcacttgt tgtaaacgac cactggcagt
    24121 tagcgcggtt ttgaagtcga tgatggcgtg ccagatctca tcgattccac gtttttccag
    24181 tgcgctacaa gtcagacccc gtggctgcca ttcgtcgtat ttacgtcgca gaatatgcag
    24241 ggcactctcg tacatatgcc gggcaatggc gacattggta tggttatcgc catcgtcttt
    24301 gttgataacg atcagatcag ccacttccat cagccctttt ttaatgccct gcagatcatc
    24361 gccgccaccg gcaatttgca acgagataaa acagtccacc atgcgggcga cttctgtttc
    24421 cgactgcccg acgccaaccg tttcgacaat cactacgtca taacccgctg cttcgcataa
    24481 cagcattaat tcccgcgctc gctgactggc accgcccaga tgaccggagg atggtaccgg
    24541 gcgaataaac gccgcttcgg cacgcgccag gtcattcatg cgggttttat ccccgagaat
    24601 gctaccgcca gtgaccgggc tgctgggatc gaccgcaata accgcgacct ttaatccctc
    24661 tcgaatcaac aacatgccaa aggcctcaag aaaggtactt ttccccgcgc cgggggtgcc
    24721 ggtaacgccc agtcgcaggg tgttaccgca gtacggcata atggcatcaa gcagctgcgt
    24781 acttagtgcc tgatgacgcg ggtgacggct ttccaccagc gtcatggcct gggcgagtgt
    24841 ggcatgctca ccctgacgta agcggcgaat actttctgcc agcgtggctt cattaatcat
    24901 gatgctggct tatcagattc agtacgtcgc gcacactgtc gagcataggt gtacctggac
    24961 cataaatcgc cgccacgccg cgctcttgca ggaaggcgta atcctgcggt ggaatgacgc
    25021 caccggcgac tacgcagata tcttcgcgtc cccatttttt cagcgcttcg accagttccg
    25081 ggatcagcgt tttatgaccg gcagccagtg aggatgcgcc cactacgtga acgtcatttt
    25141 ctactgccag gcgggcgatc tcttcaggtg tagagaacat cgggcttaaa tctacgtcga
    25201 aaccgagatc ggaataggcg ctggcgatca ctttcgcgcc gcgatcgtgt ccatccaggc
    25261 ccattttggc gatcagaatg cgcgggcgac gaccattgtc ggcaaggaac tgctccgttt
    25321 gcgcaacaat ggcatcgaac tcggaggccg atttctcaga ttgatgatag ctttgcgcaa
    25381 tcacgccggt aacacactgg cttggcacca gataacggtc gaaagcggct tccagcgcat
    25441 cggaaatttc acccagggtg gcgcgaacgc gagcggcatt aacagcggca gccagcaggt
    25501 tttcgttatg ctgtgcggcg tgagtcaggg cgttcaacgc ggcggttacg gcggcatcat
    25561 cacgggtggc gcgaatgcgt tccagcgaag caatttgctc gttacgcacc atcacgttgt
    25621 cgatctcaag tacatcggtt tcgtcttcgt gatccagttt gtacttgttg acaccaacga
    25681 tgacacgctt gccctggtcg atcagcgact gttcgcgcgc tgaggcctct tcgatcattc
    25741 gttttggcag acctgcttcg atcgctttcg ccatgccacc ggcttcgtcg atctgttgga
    25801 taatagctct ggcttgtttg acgatttgat cggtcagcga ctcaatgtaa taggatccgg
    25861 ccagtggatc gacggtgcgg cagagttctg attcttcctg gatgatgatc tgggtgttgc
    25921 gggcaatgcg tgctgagaaa tcggtaggca aaccaagcgc ttcgtcaaag gcgttggtat
    25981 gcagtgactg agtaccgccc agcgtcgcag ccagcgcttc aatggtggtg cggataacgt
    26041 tgttatacgg atcctgttca gtcaggctcc agcctgaggt ctggcagtgg gtacgcagcg
    26101 ccagtgattt cgggtcctgt gcgccaaatc cactgaccgc ttcgctccat aaataacgtg
    26161 ccgcacgcaa catggcgacg ttcataaaca gatccatgcc gatgccgaag aagaacgaca
    26221 ggcgaggagc gaagtcatca attttcagtc cggcagagat tgctgctttg atgtactcaa
    26281 tcccatcagc gagcgtaaat gctacctgct gcacgcagtt ggcacccgct tcacccatgt
    26341 ggtaaccgct gatactgatg gtattaaatc gcggcatgtt gccggaacac caggcgatga
    26401 tgtcggcgat aatgcgcatt gacggttttg gcgggtaaat ataggtgttg cggcagaggt
    26461 actctttgag aatatcgttt tgaatggtgc cggtcagttt atcaggtgta acaccttgct
    26521 cttccgcggc gatgatataa aacgccagta ctggtagcac tgcgccattc atggtcatcg
    26581 aaaccgacat tttatccagc gggatctggt cgaacaggac tttcatatct tccacggtgt
    26641 cgatagcgac gcccgttttg ccgacgtcac ccgccacgcg tgggttatcg gagtcgtagc
    26701 cacggtgggt ggcaaggtca aacgcaacgg aaagaccttt ttgcccggcg gccaggttac
    26761 ggcgataaaa agcgttggac tcttttgctg ttgaaaaacc agcatactga cggatggtcc
    26821 acggttgggc ggtatacata gtggcacgcg ggccacgaac gtagggcggc aaaccaggaa
    26881 gggtacctgt cacctccaga ttatcgagat cggcttcggt atacagcggc ttgatggcga
    26941 tcccttccgc ggtttgctga accagcgagt cgacagtttt ctcccgacgg ctcaattcct
    27001 tgttggcaag ctgttgccac tcctgctcgt tagacattat ttgctccgta aaacgcaatc
    27061 aaaatgaggg tatttgcgct tacggtgagc tttttacgtg ggtttgtcgc cagcaaaaaa
    27121 aatgcattca gacgtcgttt tttgccgcct gagatttgtc tattttgtga tgtgattcaa
    27181 ggaatacaaa aagaagtgag gataacgcct gatatgtgct atcaggcatt atttgatgga
    27241 ttaatcctga cgaaggactt tgtgaccata atcgagcaac gcatcagtga ctttacgcat
    27301 catgcggctt tcaggagcaa agcggtgcca gtagagcatc cggcgttgaa atagccctgg
    27361 cgttaagtca atcagttcac cgctggctag ttctttctcg atttgcaggt gcgggatcat
    27421 acagcaggtg gtgccctggc gagcaagttg cacgaacgct tctgaagaat taacgatatg
    27481 gcagggcacg ctgcctggag gcagatcgaa gttttgctgc aaaaaggcct ggtgcatatc
    27541 gtcaagatgg tcaaacgcga ccactggcgc tttcagtaat gccgaacgcg ttacgccgtt
    27601 agggaaatat ttttcggcaa agggttttga gctgacgaac agatagtcga gcgcaccaag
    27661 tttatcgaca agacaactcg gcagcgcctg atgttgaata ctcaccgcgc cgaccacttc
    27721 gccgcgacgc agacgttcct gagtgcgggt ttcatcttct acctgcaagt tgaggcggat
    27781 aggcgaatca gccaatacag gagccagtgc cggaagcaac cacgtcgcca gactgtcggc
    27841 attgaccgcc agtgaaagca gcagcggagt cgaaccggtt tgttcatcgc ccagccactc
    27901 ttcttccagc aactccacct ggcgcagcag tgccagcagt ttttgccctt gttccgtcgg
    27961 gcgcggcggt acggtacgca ccaacagcgg ctgcccgaac atattttcca gttgcttaat
    28021 gcgctgtgag acggctgatt gtgtaatgca cagcttttgt gccgcgcgct caaatcctcg
    28081 ttcacgtatc accgcatcca gtgcctgtaa tgttctgtag tccgggcgtt tcattgctgt
    28141 tgtctgctcc tggtaaatgc tttttcctgc actatgacac aattttgtgt caggttgcat
    28201 ataccgcagc cgtcggcggg taacatttgt atcaatggtt aaacgcgcag cttaaaagat
    28261 cagggatttg cgctattgca tttcgcttag caacttcgcg ccctggttat gttgaatggc
    28321 gtggcgttat tgcctctgtt cgcaccgtgg agcggcatac ccagaccgtt gtatgcttta
    28381 tttgcggggc gaaaggggat gcccgccatt gcgcgatatt ttatggatga gttaaccacg
    28441 cggcttgcca acggggtctg aatcgctttt tttgtatata atgcgtgtga aatttcatac
    28501 cacaggcgaa acgatcatga cgcaggatga attgaaaaaa gcagtaggat gggcggcact
    28561 tcagtatgtt cagcccggca ccattgttgg tgtaggtaca ggttccaccg ccgcacactt
    28621 tattgacgcg ctcggtacaa tgaaaggcca gattgaaggg gccgtttcca gttcagatgc
    28681 ttccactgaa aaactgaaaa gcctcggcat tcacgttttt gatctcaacg aagtcgacag
    28741 ccttggcatc tacgttgatg gcgcagatga aatcaacggc cacatgcaaa tgatcaaagg
    28801 cggcggcgcg gcgctgaccc gtgaaaaaat cattgcttcg gttgcagaaa aatttatctg
    28861 tattgcagac gcttccaagc aggttgatat tctgggtaaa ttcccgctgc cagtagaagt
    28921 tatcccgatg gcacgtagtg cagtggcgcg ccagctggtg aaactgggcg gtcgtccgga
    28981 ataccgtcag ggcgtagtga ccgataacgg caacgtgatc ctcgacgtcc acggcatgga
    29041 aatccttgac ccgatagcga tggaaaacgc cataaatgcg attcctggcg tggtgactgt
    29101 tggcttgttt gctaaccgtg gcgcggacgt tgcgctgatt ggcacacctg acggtgtcaa
    29161 aaccattgtg aaatgatctg acgggggaac ctcccccgtt aaaaaaattc tcttcattaa
    29221 atttggtgac atgtgtcacg tttttacctg gatattgtcg attgctctaa ataaatcctc
    29281 taaaccagca tattcaacca agaaatacct ttgcgtgata tttcctcaac atcgcgacgc
    29341 aaacgttcat attgccgcaa tattattttt tgatatgttg aaaggcggat gcaaatccgc
    29401 acacaacatt tcaaagacag gattgggtaa atggcaaagg tatcgctgga gaaagacaag
    29461 attaagtttc tgctggtaga aggcgtgcac caaaaggcgc tggaaagcct tcgtgcagct
    29521 ggttacacca acatcgaatt tcacaaaggc gcgctggatg atgaacaatt aaaagaatcc
    29581 atccgcgatg cccacttcat cggcctgcga tcccgtaccc atctgactga agacgtgatc
    29641 aacgccgcag aaaagctggt cgctattggc tgtttctgta taggaacaaa ccaggttgat
    29701 ctggatgcgg cggcaaagcg cgggatcccg gtatttaacg caccgttctc aaatacgcgc
    29761 tctgttgcgg agctggtgat tggcgaactg ctgctgctat tgcgcggcgt gccggaagcc
    29821 aatgctaaag cgcaccgtgg cgtgtggaac aaactggcgg cgggttcttt tgaagcgcgc
    29881 ggcaaaaagc tgggtatcat cggctacggt catattggta cgcaattggg cattctggct
    29941 gaatcgctgg gaatgtatgt ttacttttat gatattgaaa acaaactgcc gctgggcaac
    30001 gccactcagg tacagcatct ttctgacctg ctgaatatga gcgatgtggt gagtctgcat
    30061 gtaccagaga atccgtccac caaaaatatg atgggcgcga aagagatttc gctaatgaag
    30121 cccggctcgc tgctgattaa tgcctcgcgc ggtactgtgg tggatattcc ggcgctgtgt
    30181 gatgcgctgg cgagcaaaca tctggcgggg gcggcaatcg acgtattccc gacggaaccg
    30241 gcgaccaata gcgatccatt tacctctcca ctgtgtgaat tcgacaacgt ccttctgacg
    30301 ccacacattg gcggttcgac tcaggaagcg caggagaata tcggcctgga agttgcgggt
    30361 aaattgatca agtattctga caatggctca acgctctctg cggtgaactt cccggaagtc
    30421 tcgctgccac tgcatggtgg tcgtcgtctg atgcacatcc acgaaaaccg tccgggcgtg
    30481 ctaactgcgc tgaacaaaat cttcgccgag cagggcgtca acatcgccgc gcaatatcta
    30541 caaacttccg cccagatggg ttatgtagtt attgatattg aagctgacga agacgttgcc
    30601 gaaaaagcgc tgcaggcaat gaaagctatt ccgggtacca ttcgcgcccg tctgctgtac
    30661 taattcccct tctctgaaaa ttaacgggca ggtcactgac ttgcccgttt ttttatccct
    30721 tctccacacc gttgccgcgt tgcgcattct actttgcgag tctgctccag aaaataaatc
    30781 aaagtcagat caatgcgttg tgtttattta atggggattt acgatggcgg gacagcatgg
    30841 ggctgtaacg gtgaagccct caccgaagcg agggcttaaa ggagaagggt tatgatgcga
    30901 cttgtcatca tactgattgt actgttactg ataagtttcg gagcttatta acagtcagtc
    30961 tcaggggagg agtcatcctc ccttaccctt actcactaaa ttaggtcaaa gaatcaacga
    31021 tgtcaatcag ggcgatgcgg ggtgcatcgc cctgaaccga actttttgat agccacttca
    31081 tcgcccacgt ttactgccgt aattaatccc ttattcctgc aatgtatgtc tgccttacat
    31141 gacgttttac gagtgcgctc gaagaaaaga tttaaaggta gatcaatgcg ttgtggttat
    31201 ttaaagagag tttacgatgg cagggcagca tggggctgta acggtaaagc cctcaccgaa
    31261 gcgagggctt gaaggagaag ggttatgatg cgacttgtca tcatactgat tgtactgtta
    31321 ctgataagtt tgccagctta ttaacagtca gtctcagggg aggagtcatc ctcccttacc
    31381 cttactcact aaattaggtt aaagaatcaa cgatgtcaat cagggcgatg caggtgtatc
    31441 gcccttacca ctcccagact ttcgacggtg taaccaccgc aggaagaggg atatcccact
    31501 cttcaacggg gagtttttcc accaactgac aatcatgcgc ataacccacc ggttgcgttt
    31561 tatagtgctg ccagttttgt aaggtccgat cataaaaacc accgcccatt cccaggcgct
    31621 gaccgtactc atcaaaggcg accagcggtg tgatcagcac gtctaatcgg gaaaggggga
    31681 gcacgtcacg cacatccagt tttggctcat ggattttcaa cctgttcatc accagttcgc
    31741 tttgcggatg gtaattcagg aacagcaaat taccggcact aaagggatgc aaaactggaa
    31801 gatatacgcg cttaccggcg cgccagagtt gttctataag cggctgggtg tcgagttcac
    31861 catcaaaaga gaggaataca gcgaccgtat gcgccatcac caccggggga taagtcatca
    31921 tccgggtagc ggcttgttga cccatttcct gctgttgttc cggcgttaac gcacgacgac
    31981 gttgccgaat cattttgcgg atttcttgtc gggataatgt cagtgggagt tctggtagtt
    32041 gcgtcatggc tggtgccaga taagaaggga atctccgaga tgccggcgca ggctgtaacc
    32101 cttgaaccct tggttcaagg tgaatgtgtc gtcgcagttt taaggcttct cggacggacc
    32161 gagcatgctc accaaccgcg gagcgccaca ttcttgtggt atgaaatatc ggctcagggg
    32221 actggcccgc ttgcgaacat ctcagagaaa ttttgtcttc acggttactc taccacagta
    32281 aaccgaaaag tgtcattcaa agttttggtt agttttttcg gtgatgcgac cttgttcaag
    32341 taacgcttgt tctatggtct gctgcagcat ccgaatacgc tgttccatac ttgccgcgta
    32401 gtcacgagtc ttcgcttttt cttgcgctaa ctcatagctg atattcaatg cggcaatgaa
    32461 gaccaactgt tcagtatttg tgactctagt gcgttctttc agatcttgca accgttggtt
    32521 cagatcgtcc gctgcctgat tcaacgcatc cctttggtca ggcgggcagt tcacacgcag
    32581 tgaacggcca aaaatttgga tatcgacggg ttgtgcagac atgccacctt cctgctgatt
    32641 gactgcgctg ccttcgtctt tagacccatg gtctgcgaag gggcgacact atagctaccc
    32701 tgatgagaag agacaagccc ttttctggtc caccagggcc caaagtggta gcatatcatg
    32761 aatattcctc cctttgacga cgaatgctta tgtctataca gaacgaaatg cctggttaca
    32821 acgaaatgaa ccagtatctg aaccaacaag ggacgggtct gacccctgct gagatgcatg
    32881 gtttaatcag tgggatgata tgtggcggta acgatgacag ctcatggcta ccgctacttc
    32941 acgacctgac gaacgaaggc atggctttcg gtcatgagct ggcacaggca ttgcgtaaaa
    33001 tgcactctgc caccagcgat gccctgcagg atgacggctt cctttttcag ctttatctgc
    33061 ctgatggcga tgatgtcagc gttttcgatc gggctgatgc attggcaggt tgggtcaatc
    33121 acttcctgct tggtcttggt gttacgcaac cgaagctgga taaagtgacc ggcgaaaccg
    33181 gtgaagctat cgacgatctg cgtaacattg cgcaactggg ttacgacgaa gacgaagatc
    33241 aggaagagct tgaaatgtcg cttgaagaga tcatcgaata cgttcgtgtt gccgcgctgt
    33301 tatgccacga cacctttact catccgcaac cgaccgcgcc agaagtacaa aaaccgactc
    33361 tacactaaaa acaaaaaacg taaggagagt gttatgagtg agatatcccg gcaagagttt
    33421 cagcgtcgcc gtcaggccct ggtggagcaa atgcaacccg gcagcgccgc gctgattttt
    33481 gctgcaccag aagtaacacg tagcgccgac agcgaatacc cctatcgtca gaacagtgac
    33541 ttctggtact tcaccggctt taacgaaccg gaagcggtgt tggtgctgat taaaagcgat
    33601 gacactcata accacagcgt tctgtttaac cgcgttcgcg acctgacggc ggagatctgg
    33661 tttggccgtc gcttaggcca ggatgccgcg ccagagaaac tgggcgttga ccgcgcactg
    33721 gcattcagcg aaattaatca gcaactttat caactactta atggtctgga tgtggtttac
    33781 catgctcagg gcgaatatgc atatgctgat gaaatcgtga acagtgcgct ggaaaaactg
    33841 cgtaaaggtt cgcggcaaaa tctcaccgca ccggcaacga tgatcgactg gcgtcctgtt
    33901 gttcatgaaa tgcgcctgtt caaatcgcca gaagagattg ccgtactccg ccgcgcggga
    33961 gaaatcaccg ccatggcaca tacacgggcg atggaaaaat gccgtccggg aatgttcgag
    34021 taccatctgg aaggcaaaat tcaccacgaa tttaaccgcc acggtgcgcg ctatccgtcc
    34081 tataacacca ttgtaggcag cggtgaaaac ggctgcattt tacactacac cgaaaacgag
    34141 tgtgaaatgc gcgacggcga cctggtgttg attgacgcag gctgcgaata caaaggttac
    34201 gcaggcgata tcacccgtac cttcccggtc aacggcaaat tcacccaggc ccagcgcgaa
    34261 atctacgaca ttgtgctgga gtctcttgaa accagcctgc gcctgtatcg tccgggaacc
    34321 tccattctgg aagtcaccgg tgaagtggtg cgcattatgg ttagcggcct ggtaaaactc
    34381 ggcatcctga aaggtgatat tgatgaactg atcgctcaga acgcccatcg tcctttcttt
    34441 atgcatggcc ttagccactg gttaggactg gatgtccatg acgttggcgt ttatggtcag
    34501 gatcgctcgc gcattctgga accggggatg gtactgaccg tagagcctgg tctgtatatc
    34561 gcgccggatg cggatgtgcc agaacaatat cgcggtatcg gcattcgtat tgaagacgac
    34621 attgtgatta ccgaaaccgg caacgaaaac ctcaccgcca gcgtggtgaa aaagccggaa
    34681 gaaatcgaag cgttgatggc tgctgcgaga aagcaatgag cgtaatcatc gtcggtggcg
    34741 gcatggcggg cgcgacgctg gcgctggcta tttcccggtt aagtcacggg gcgctgccgg
    34801 tacatttgat tgaagcgact gcgccagagt cacatgctca tccgggcttt gatggacgag
    34861 cgatagcgct ggcggcgggt acctgtcagc aactggcgcg catcggcgtt tggcaatctc
    34921 tggcggattg cgcaactgcc atcaccaccg tgcatgtcag cgatcgaggt cacgctggat
    34981 ttgtcaccct cgccgcagaa gattaccaac tggcggcgct gggacaggtt gtcgaattgc
    35041 acaatgtcgg gcaacggctg tttgcattgc tgcgtaaagc acctggcgta acgctgcatt
    35101 gccctgatcg cgtggctaac gttgcccgta ctcagagtca cgttgaagtg acgctggagg
    35161 gtggcgagac gctgacgggc cgcgtgctgg tagccgctga tggcacccat tcagcgttag
    35221 ccactgtgtg cggcgttgac tggcagcagg agccttacga acaactggct gtgattgcca
    35281 acgttgcgac ttccgttgcg catgaagggc gcgcttttga acgctttacg caacatggcc
    35341 cgctggcgat gttgccgatg tctgacggac gctgttcgct ggtctggtgt catccactgg
    35401 aacggcgcga agaggtgctg tcgtggagcg acgagaagtt ttgccgtgaa ctccagtcgg
    35461 cctttggctg gcgacttggg aaaattaccc acgctggtaa acgcagtgct tatccgctgg
    35521 cgttaaccca cgccgccaga tctattaccc atcgtaccgt gctggtgggc aatgcggcgc
    35581 aaaccctgca cccgattgcc gggcaagggt tcaacctcgg tatgcgagat gtcatgagtc
    35641 ttgcggaaac cctgactcag gcgcaggagc gcggagaaga catgggtgat tacggcgtat
    35701 tgtgtcgtta tcagcagcgt cgacagagcg atcgcgaagc aaccattggc gtcacggaca
    35761 gccttgtaca tctttttgcc aaccgttgga cgccactggt tgtcgggcgc aacatcgggc
    35821 tgatgacgat ggaattattc accccggcac gcgatgtgct ggcgcagcgc accctcggtt
    35881 gggtggcgcg ttgaaggcgg ttattaagga ataaacaatg caaagtgttg atgtagccat
    35941 tgttggcggc ggcatggtgg ggctggcggt tgcctgcggc ttacagggta gcggcttacg
    36001 cgttgccgta ctggagcagc gcgtaccgga acctctggcg gcggatgcac caccacaact
    36061 gcgcgtttcg gctatcaatg ccgccagcga aaaattactc acccgtcttg gcgtctggca
    36121 ggacattctc tctcgccgag ccagctgtta tcacggtatg gaagtgtggg acaaagacag
    36181 ttttggtcac atctcgtttg acgatcaaag catgggctat agccatcttg ggcacatcgt
    36241 tgaaaactca gtgattcact acgcgctgtg gaacaaagcg cagcagtcgt cagatatcac
    36301 tcttttggcc cccgcagaat tacagcaggt cgcctgggga gaaaatgaaa ccttcctgac
    36361 gctgaaagat ggcagtatgt taacggcgcg tctggtgatt ggcgcggacg gcgctaattc
    36421 ctggttgcgc aacaaagccg atattccgct gactttctgg gattatcagc atcacgcgct
    36481 ggtagcgacc atacgcacgg aagaaccgca tgatgcggtg gcgcggcagg ttttccatgg
    36541 cgaaggcatt ctggcctttt taccgcttag cgatccgcat ctttgctcga ttgtctggtc
    36601 actgtcgcca gaggaagcgc agcggatgca gcaggcaagt gaagacgaat ttaatcgcgc
    36661 gttaaatatc gcttttgata atcgcctggg cttatgcaag gttgagagcg cgcgtcaggt
    36721 gttcccactg acggggcgtt atgcgcgcca gtttgccgcg caccgtctgg cgctggtggg
    36781 cgacgccgcg cataccattc acccgctggc ggggcagggg gtaaatcttg gctttatgga
    36841 tgctgcagag ctgattgctg aactgaaacg gttgcatcgt cagggtaaag acatcgggca
    36901 gtacatttat ctgcgtcgct atgagcgtag ccgcaagcac agtgcggcgc tgatgctggc
    36961 tggtatgcag ggattccgcg atctgttttc tggtaccaat ccggtgaaaa aactgctgcg
    37021 tgatattggt ctgaaactgg ccgacacgct tcctggcgtt aaaccacaac ttatccgcca
    37081 ggcaatggga ttaaacgatt tgcctgaatg gctgcgttaa aaatttctct tctgctgttt
    37141 atttgatacc catcacactt tcatctcccg gttttttcgc cgggagattt tcctcatttg
    37201 aaataaacta atttcacctc cgttttcgca ttatattttc taatgtcacg aatttttgat
    37261 ttagtgtttt ttgacatttt ttcagctctt aatattgcct tattcaaatt aactttctca
    37321 tcacatcatc tttatataga aacttgtgta ttttttggtt ttttattctg ttgcgatttt
    37381 ggcgtttttt aaccataagc taatgtgatg atcaatttta ccttatggtt aacagtctgt
    37441 ttcggtggta agttcaggca aaagagaacg attgcgttgg ggaccgggag tggctccgat
    37501 gctgggtttc gtggtgataa tttcaccatg aaaaagttgt cagccccgct tattcaatga
    37561 ggacaagatg gcacaacaga ctcctttgta cgaacaacac acgctttgcg gcgctcgcat
    37621 ggtggatttc cacggctgga tgatgccgct gcattacggt tcgcaaatcg acgaacatca
    37681 tgaggtacgt accgatgccg gaatgtttga tgtgtcacat atgaccatcg tcgatcttcg
    37741 cggcagccgc acccgggagt ttctgcgtta tctgctggcg aacgatgtgg cgaagctcac
    37801 caaaagcggc aaagcccttt actcggggat gttgaatgcc tctggcggtg tgatagatga
    37861 cctcatcgtc tactacttta ctgaagattt cttccgcctc gttgttaact ccgccacccg
    37921 cgaaaaagac ctctcctgga ttacccaaca cgctgaacct ttcggcatcg aaattaccgt
    37981 tcgtgatgac ctttccatga tcgccgtaca agggccgaat gcgcaggcaa aagctgccac
    38041 actgtttaat gatgctcagc gtcaggcggt ggaagggatg aaaccgttct ttggcgtgca
    38101 ggcgggcgat ctgtttattg ccaccactgg ttataccggt gaagcgggct atgaaattgc
    38161 gctgcccaat gaaaaagcgg ccgatttctg gcgtgcgctg gtggaagggg gcgttaagcc
    38221 atgcggtctg ggcgcgcgtg acacgctgcg tctggaagcg ggcatgaatc tttatggtca
    38281 ggagatggac gaaaccatct ctccgttagc cgccaacatg ggctggacta tcgcctggga
    38341 accggcagat cgtgacttca tcggtcgtga agccctggaa gtgcagcgtg agcatggcac
    38401 agaaaaactg gttggtctgg tgatgaccga aaaaggcgtg ctgcgtaatg aactgccggt
    38461 acgttttacc gatgcgcagg gcaaccagca tgaaggcatt atcaccagcg ggactttctc
    38521 cccgacgctg ggttacagca ttgcgctggc acgcgtgccg aaaggtattg gcgaaacggc
    38581 gattgtgcaa attcgcaacc gtgaaatgcc ggttaaagtg acaaaacctg tttttgtgcg
    38641 taacggcaaa gccgtcgcgt gatttacttt tttggagatt gattgatgag caacgtacca
    38701 gcagaactga aatacagcaa agaacacgaa tggctgcgta aagaagccga cggcacttac
    38761 accgttggta ttaccgaaca tgctcaggag ctgttaggcg atatggtgtt tgttgacctg
    38821 ccggaagtgg gcgcaacggt tagcgcgggc gatgactgcg cggttgccga atcagtaaaa
    38881 gcggcgtcag acatttatgc gccagtaagc ggtgaaatcg tggcggtaaa cgacgcactg
    38941 agcgactccc cggaactggt gaacagcgaa ccgtatgcag gtggctggat ctttaaaatc
    39001 aaagccagcg atgaaagcga actggaatca ctgctggatg cgaccgcata cgaagcattg
    39061 ttagaagacg agtaacggct ttattcctct tctgcgggag aggatcaggg tgaggaaatt
    39121 tacacctcac cctcactctc ttcgcaagga gagaggttca caattcactg cacgtttcag
    39181 gaaccatcgc tcatgacaca gacgttaagc cagcttgaaa atagcggcgc ttttattgaa
    39241 cgccatatcg gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt tggcgcacaa
    39301 tcgttaaacg cgctgaccgg ccagattgta ccgaaagata ttcagcttgc gaccccgccg
    39361 caggttggcg caccggcgac cgaatacgcc gcgctggcgg aactcaaggc tattgccagt
    39421 cgcaataaac gcttcacgtc ttacatcggc atgggttaca ccgccgtgca gttaccgccg
    39481 gttatcctgc gtaacatgct ggaaaatccg ggctggtata ccgcgtatac tccgtatcaa
    39541 cctgaagtct cgcagggccg ccttgaggca ctgctcaatt tccagcaggt aacgctggat
    39601 ttaaccgggc tggatatggc ctctgcttct cttcttgacg aagccaccgc tgccgccgaa
    39661 gcaatggcga tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt cttcgtggct
    39721 tccgatgtgc atccgcaaac gctggatgtg gtccgtactc gtgccgaaac ctttggtttt
    39781 gaagtgattg tcgatgatgc gcaaaaagtg ctcgaccatc aggacatctt cggcgtgctg
    39841 ttacagcagg ttggcactac cggtgaaatt cacgactaca ctgcgcttat cagcgaactg
    39901 aaatcacgca aaattgtggt cagcgttgcc gctgatatta tggcgctggt gctgttaact
    39961 gcgccgggta aacagggcgc ggatattgtt tttggttcgg cgcaacgctt tggcgtaccg
    40021 atgggttacg gtggcccaca cgcggcattc tttgcggcga aagacgaata caaacgctca
    40081 atgccaggcc gtattatcgg tgtatcgaaa gatgcagctg gcaataccgc gctgcgcatg
    40141 gcgatgcaga ctcgcgagca acatattcgt cgtgagaaag cgaactccaa catttgtact
    40201 tctcaggtgc tgctggcaaa catcgccagc ctgtatgccg tttatcacgg cccgattggc
    40261 ctgaaacgta tcgctaaccg cattcaccgt ctgaccgata tcctggcggc aggcctgcaa
    40321 caaaaaggtc tgaagctgcg ccatgcgcac tatttcgaca ctctgtgtgt ggaagtggcc
    40381 gacaaggcgg gcgtactggc gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatc
    40441 ctgaacgcgg ttgggatcac ccttgatgaa accaccacgc gcgaaaacgt gatgcagctt
    40501 ttcagcgtac tactggggga taaccacggg ctggacatcg acacgctgga caaagacgtg
    40561 gctcacgaca gccgctctat ccagcctgcg atgctgcgcg acgacgaaat cctcacccat
    40621 ccggtgttta atcgctacca cagcgaaacc gaaatgatgc gctatatgca ctcgctggag
    40681 cgtaaagatc tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac catgaaactg
    40741 aacgccgccg ccgagatgat cccaatcacc tggccggaat ttgccgaact gcacccgttc
    40801 tgcccgccgg agcaggccga aggttatcag cagatgatcg cgcagctggc tgactggctg
    40861 gtgaaactga ccggttacga cgccgtttgt atgcagccga actctggcgc acagggcgaa
    40921 tacgcgggcc tgctggcgat tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat
    40981 atctgcctga tcccggcttc tgcgcacggc actaaccccg cttctgcaca tatggcagga
    41041 atgcaggtgg tggttgtggc gtgtgataaa aacggaaata tcgatctgac tgatctgcgc
    41101 gcgaaagccg aacaggcagg tgataatctc tcctgcatca tggtgaccta tccttccacc
    41161 cacggtgtgt atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt
    41221 caggtttacc ttgatggcgc gaacatgaac gcccaggttg gcatcacctc gccgggcttt
    41281 attggcgcag acgtttcaca ccttaacctg cataaaactt tctgcattcc gcacggcggt
    41341 ggtggtccgg gtatgggacc gatcggcgtg aaagcgcatc tggctccgtt tgtaccgggt
    41401 catagcgtgg tgcaaatcga aggcatgtta acccgtcagg gcgcggtttc tgcggcaccg
    41461 ttcggtagcg cctctatcct gccaatcagc tggatgtaca tccgcatgat gggcgcagaa
    41521 gggctgaaaa aagcaagcca ggtggcaatc ctcaacgcca actatattgc cagccgcctg
    41581 caggatgcct tcccggtgct gtataccggt cgtgacggtc gcgtggcgca cgaatgtatt
    41641 ctcgatattc gtccgttgaa agaagaaacc ggcatcagcg agctggatat tgccaagcgc
    41701 ctgatcgact acggtttcca cgcgccgacg atgtcgttcc cggtggcagg tacgctgatg
    41761 gttgaaccga ctgaatctga aagcaaagtg gaactggatc gctttatcga cgcgatgctg
    41821 gctatccgcg cagaaattga ccaggtgaaa gccggtgtct ggccgctgga agataacccg
    41881 ctggtgaacg cgccgcacat tcagagtgaa ctggtcgccg agtgggcgca tccgtacagc
    41941 cgtgaagttg cggtattccc ggcaggcgtg gcagacaaat actggccgac agtgaaacgt
    42001 ttggatgatg tttacggcga ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac
    42061 cagtaattca ctgattcgac tatcttctaa aggcgcttcg gcgccttttt agattgttaa
    42121 taaagtgcgc tttgtgtgtt tcggctgagg cgtaatataa aatattgcga gctcaatata
    42181 ttaaaagagt gccaggcctg atgagcgtag cgcatcaggc acatcggcta attacagatt
    42241 ctcgccgttg ctggcaatca cttctttgta ccagttaaag ctcttcttac gtgaacgcga
    42301 catatcgcca gtaccgtcgt catgtttatt cacatagata aagccgtagc gtttgctgta
    42361 ctgcccggta gtgaacgaca cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc
    42421 cacgccatcg taagttactg cttttttcat ctcttcaata tgggcgcgca ggtagtcaat
    42481 gcggtagtcg tcgttgatgc tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt
    42541 ttcgacaata aacagcggct tctggtaacg ctcatacagt tcgcaaagtg cgtaacgcag
    42601 acccaccgga tcaatctgcc agccccagtc agatgcttta acatacgggt ttggtacgct
    42661 gccttcaaaa ccagagatcg catcgccggt gccgccttcg gccttcactg cgttggtcat
    42721 gtaatagctg aaaccaagat aatcgcaggt gccttcgcgc agtacatcga gatcgctatc
    42781 ttccattttg atgttaaatc cgcgacgctc ccactcgttc aacacatacg acgggtaata
    42841 gccgcgtagc tgcacatcgg taaagacgta gcgttcgcgc atcgactcct gagcgaacat
    42901 cacatcgtcc gggttacatg agtagggata gagcggcacc atcgccagca tacagccgac
    42961 tttcatctcc aggttaatgc gacgcgcggc tttcactgcc agggcgctgg cgacaaactg
    43021 gtgatgcagc acctgataca tcgtctcttc cgggttttca tgctcggtat acaccacgcc
    43081 ggagcagcag taaccgaaca gcggtgcacg ccagttacgc tggttgttaa tttcgttgaa
    43141 ggtcatccag tatttgactt tgtgcttata gcgttcaaat accacttccg cgaaacgtac
    43201 aaagaaatca accactttac ggttggtcca gctaccgtat tgctgcacca gatgcagcgg
    43261 catttcaaag tgggagaggg tgatcaccgg ttcgatgttg tatttcagca gttcatcgaa
    43321 cagagagtcg tagaacttca gcccttcttc gtttggctga gcttcatcgc cttttggaaa
    43381 aatgcgcgtc caggcaatgg atgtacgaaa acatttgaag cccatttcgg caaatagctt
    43441 gatgtcttcc ttatagtgac cataaaaatc aacggcttca tggtttggat agtatttgcc
    43501 tggcaagact tctttggtta tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc
    43561 acaaatgctc ggcccttttc cgcctttgtt ccagccgcct tcgacctgat gagcggcaac
    43621 tgcgccgccc cataagaaat ctttcggtaa ggtgagtttt ttcactatca taggctccag
    43681 atgagtcaat tcttacagga aatagtacca tgcagccaaa cgacatcact ttttttcaac
    43741 gtttccagga tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat
    43801 cgcacttcaa aacgggtgat gtgcttcgtg tcggacgttt tgaagatgac ggttattttt
    43861 gcacgattga agtcaccgca acctcaaccg taacgctgga tacgctgacg gaaaaacatg
    43921 cagagcagga aaatatgacc ctgactgaac tgaaaaaggt cattgccgac atctatcccg
    43981 gccagacaca gttttatgtg attgaattta aatgccttta aattgaactc acgcctgtta
    44041 gttgaaattt cgcatttaat ttcatgattt ttaggagatt agtctcaaat cagaatttat
    44101 tttagctaac aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc
    44161 gtgtacgttt ctccggagta agttatggtt cagaagcccc tcattaagca gggatattcg
    44221 ctggcagagg aaattgccaa cagcgtcagt cacggcattg ggttggtgtt tggtatcgtt
    44281 gggctggtgt tgctactggt tcaggcggtg gatctcaatg ccagcgccac agcgataacc
    44341 agctacagcc tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac
    44401 gccattcctc atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac
    44461 ctgttgattg ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg
    44521 cgcgggttga tgattgttat ctggagcctg gcattgctgg ggattctgtt caaactgacc
    44581 atcgcgcacc gattcaaaat tttatctctg gtgacttatc tggcgatggg ctggctgtcg
    44641 ctggtggtaa tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac cttactggcg
    44701 gtaggcggcg tggtttattc gctcggggtg attttctacg tctgcaaacg cattccatac
    44761 aaccatgcca tctggcacgg cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc
    44821 tatttgtata ttgggcaggc gtaatgggta tgcctttgta ggcctgataa gacgcgccag
    44881 cgtcgcatca ggcgataaat gcctgatgcg acacagagat ttactcttcg agcgaatacg
    44941 gcagtggctc gatacacaat gtattcgcat cgtcgcgcac gcggaagatg ctgtccggtt
    45001 ccatatcgtt attcatcact acctgcacca cgacctgacc atcttccagt tttaccgcag
    45061 ccagcaccgt accagtacga cgccagttct cgcccatttt cagctctaag tcttcaccag
    45121 cttccggcag tcggctggca ctacctgtca gcaaccagag cgcgcgtttg ttggcaccac
    45181 ggaattttgc tcgcgccacc atctcttgtc cggtatagca gcctttctta aagctgatac
    45241 cgcccagcgc ctggaggttg gtcgcctgtg ggataaactg cccgctgttc gcggcatcaa
    45301 tcaccgggaa acccgcttca atgtttaatg ccagccactg ttggctattg ttcagttccg
    45361 cttcaccgcg cagtttatcg gtcagcatat tggcagtagc ttcatcggtt acgatcagga
    45421 aacgttctgc cgggtgttca aaccatagca aagtggtcgc gccttcttta actacctgtt
    45481 tttctttcga aggcagttca ctaaagatat ttgccagcgc ggcgcgcgcc tgaaaaccgg
    45541 caacaccaag cagcacacgc tcgtcgtctg gcgcgatggt cactttagag aacaccgcat
    45601 attttttcag ttcagccagc tgcggttcac gcacgctgcg ccgttcaatc catgcaaagc
    45661 catcgccgtc gcggaacaga cgtaaattgc tccacatttt acctttggcg tcgcaatggg
    45721 cggcgagcag gtgctgatca tctgtcatct ggctgacatc tgctgtcacc tggccctgca
    45781 aatatttttc gctgtccgcg ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca
    45841 gcgtcagtgg caaacgggca gaagccgtag gctgacgggg aggaaaaggt gtaaaagcca
    45901 taatagagtc ctgagttgct taacggcgta ttaataacta atggtaaaag agctatgagg
    45961 caatgcaagt gctttcacta cgcgatctgt gccttctgcg aggcgacttc ccaaaaaatg
    46021 aagcccttaa attgttgtga attaatttcg cgatcgcgat tgtgtttact gatattcccg
    46081 taaaagaagt cgaaaaacac gttacaatag ccgggtactg gattttcgtg agacaggaag
    46141 aagatggaca ttaacaacaa agcccgcatt cattgggcat gccgccgtgg tatgcgcgaa
    46201 ctcgatattt caatcatgcc gtttttcgaa catgagtatg acagcttaag cgatgacgaa
    46261 aaacgcatct ttattcgtct gctggaatgt gacgatcccg atctgtttaa ctggctgatg
    46321 aatcacggta aaccggccga tgcagaactg gaaatgatgg tccgactcat ccagacacgg
    46381 aaccgggaac gtggtcctgt ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct
    46441 ttccttgctg attcatgggc tggttgccgc tgttatttta ctcatgccct ggccgctcag
    46501 ttacaccccg ttatggatgg tgttactttc gctggtggtg tttgattgcg ttcgcagcca
    46561 gcggcgtatt aatgctcgcc agggggaaat tcgcttgttg atggacgggc gtttgcgttg
    46621 gcaagggcag gagtggagca tcgtcaaagc gccgtggatg attaagagcg gcatgatgct
    46681 gcgtttacgt tctgatagcg gtaaacggca acatttatgg ctggcagccg acagcatgga
    46741 cgaagccgaa tggcgggatt tacggcggat tttgttgcaa caagagacgc aaagataaag
    46801 atgacggcaa agcatatgcg ctaagccgtc agtgatatca ggcgtaatgc tctgccattt
    46861 cgttgaggat ttgctcgcac cagctctgaa tacgctcgtc gctaaggtca tactggttag
    46921 tttcgtccag cgccagcccc acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg
    46981 taaattcata cccttccgtt ggccagtagc cgacgaactt cacgcctttg gtcgagagtt
    47041 tgtcatgcag cataccgagc gcatcgagga accactcgcc gtatcccagt tgatcgccaa
    47101 gcccatacag cgcaacaatt ttaccttcga ggttcaggtc gtcgagctga tcccagacgg
    47161 cttcccagtc ttcctggatt tcgccaaaat cccaggtcgg gatacccaga atgagcacat
    47221 cgtactgctc cattaatttc ggggagtcgt ccttgaggtt atgtaaggtc accagttctg
    47281 ggccgataat atcgcggatt ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt
    47341 aaaaaagacc catattcata acgtaagctt ctcgattctg ctgtggcttt tgcggctagt
    47401 gtatcagaat cgctttagtc acggcacaat aaggcataat ggatcaaatg agtgaccata
    47461 aggggcgcaa gtgaaacagg atctggcacg catcgagcag tttcttgatg ctctgtggct
    47521 ggaaaaaaat ctggctgaaa atacgttgaa cgcttaccgt cgcgatctgt caatgatggt
    47581 ggagtggttg catcaccgcg ggttgacgct ggcgacggcg caaagtgacg atttacaggc
    47641 attactggca gaacggctgg agggcgggta taaagccacc agctcagcgc gtttgctgag
    47701 tgcagtgcgc cgattgttcc agtatcttta tcgcgaaaag tttcgtgaag atgatcccag
    47761 tgcgcatctc gcttcaccga aattgcccca gcgtttgcca aaagatttaa gcgaagcgca
    47821 ggtcgaacgt ttattacagg caccattaat tgatcagcca ctggagctac gcgataaagc
    47881 tatgcttgaa gtgttgtatg ctaccgggct gcgtgtctct gaactggtcg ggctgacaat
    47941 gagtgatatc agcctgcgtc agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg
    48001 cctggtgccg ttaggtgaag aggcggttta ctggctggaa acctatctgg aacatgggcg
    48061 tccgtggcta ctgaatggtg tgtcaattga cgtgttgttt cccagccagc gtgcgcagca
    48121 gatgacgcga cagaccttct ggcaccgcat taaacattat gccgtgctgg cgggtatcga
    48181 cagcgaaaag ctgtcaccgc atgtgttgcg tcacgctttt gccactcact tattaaatca
    48241 tggtgcggat ttacgcgtgg tgcagatgct actgggccac agcgatctct ccaccacgca
    48301 aatttatacg catgtcgcta ccgagcgtct gcggcaactt catcaacagc atcacccgcg
    48361 ggcgtgatgt ctgaaaagaa cgggaagatt tatgaagaaa ggttttatgt tgtttacttt
    48421 gttagcggcg ttttcaggct tcgctcaggc tgatgacgcg gcaattcaac aaacattagc
    48481 caaaatgggc atcaaaagca gcgatattca gcccgcgccc gtagctggca tgaagactgt
    48541 gctgactaac agcggtgtgt tgtacatcac cgatgatggt aaacatatca ttcaggggcc
    48601 aatgtatgac gttagcggca cggctccggt caatgtcacc aataagatgc tgttaaagca
    48661 gttgaatgcg cttgaaaaag agatgatcgt ttataaagca ccgcaggaaa aacacgtcat
    48721 caccgtgttt actgatatta cctgtggtta ctgccacaaa ctgcatgaac aaatggcaga
    48781 ctataacgcg ctggggatca ccgtgcgtta tcttgctttc ccacgccagg ggctggacag
    48841 cgatgctgag aaagaaatga aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga
    48901 tgatgtgatg gcaggtaaaa gcgtcgcacc agccagctgc gacgtggata ttgccgacca
    48961 ttacgcactt ggcgtccagc ttggcgttag cggtactccg gcagttgtgc tgagcaatgg
    49021 cacacttgtt ccgggttacc agccgccgaa agagatgaaa gaattcctcg acgaacacca
    49081 aaaaatgacc agcggtaaat aattcgcgtg aaacaacaga tacaacttcg tcgccgtgaa
    49141 gtcgatgaaa cggcagactt gcccgcggaa ttgcctccct tgctgcgccg tttatacgcc
    49201 agccggggag tacgcagtgc gcaagaactg gaacgcagtg ttaaaggtat gctgccctgg
    49261 cagcaactga gcggcgtcga aaaggccgtt gagatccttt acaacgcttt tcgcgaagga
    49321 acgcggatta ttgtggtcgg tgatttcgac gccgacggtg ccaccagcac ggctctaagc
    49381 gtgctggcga tgcgctcgct tggttgcagc aatatcgact acctggtacc aaaccgtttc
    49441 gaagacggtt acggcttaag cccggaagtg gtcgatcagg cccatgcccg tggcgcgcag
    49501 ttaattgtca cggtggataa cggtatttcc tcccatgcgg gagttgagca cgctcgctcg
    49561 ttgggcatcc cggttattgt taccgatcac catttgccgg gcgacacatt acccgcagcg
    49621 gaagcgatca ttaaccctaa cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc
    49681 gtgggtgtgg cgttttatct gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg
    49741 tttgatgagc gcggcatcgc aattcctaac ctggcagaac tgctggatct ggtcgcgctg
    49801 gggacagtgg cggacgtcgt gccgctggac gctaataatc gcattctgac ctggcagggg
    49861 atgagtcgca tccgtgccgg aaagtgccgt ccagggatta aagcgctgct ggaagtggca
    49921 aaccgtgatg cacaaaaact cgccgccagc gatttaggtt ttgcgctggg gccacgtctc
    49981 aatgctgccg ggcgactgga cgatatgtcc gtcggtgtgg cgctgttgct gtgcgacaac
    50041 atcggcgaag cgcgcgtgct ggcaaatgaa ctcgatgcgc taaatcagac gcgaaaagag
    50101 atcgaacaag gaatgcagat tgaagccttg accctgtgcg agaaactgga gcgaagtcgc
    50161 gacacgctac ccggcgggct ggcaatgtat caccccgaat ggcatcaggg cgttgtcggt
    50221 attctggctt cgcgcatcaa agagcgtttt caccgtccgg ttatcgcctt tgcgccagca
    50281 ggtgatggta cgctgaaagg ttccggtcgc tccattcagg ggctgcatat gcgtgatgcg
    50341 ctggagcgat tagacacact ctaccccggt atgatgctga agtttggcgg tcatgcgatg
    50401 gcggcgggtt tgtcgctgga agaggataaa ttcgaactct ttcaacaacg gtttggcgag
    50461 ttggttaccg agtggctgga cccttcgcta ttgcaaggcg aagtggtgtc agacggcccg
    50521 ttaagcccgg ccgaaatgac catggaagtg gcgcagctgc tgcgcgatgc tggcccgtgg
    50581 gggcagatgt tcccggagcc gctgtttgat ggtcatttcc gtctgctgca acagcggctg
    50641 gtgggcgaac gtcatttgaa agtcatggtc gaaccggtcg gcggcggtcc gctgctggat
    50701 ggtattgctt ttaatgtcga taccgccctc tggccggata acggcgtgcg cgaagtgcaa
    50761 ctggcttaca agctcgatat caacgagttt cgcggcaacc gcagcctgca aattatcatc
    50821 gacaatatct ggccaattta gcgtcatctt ctctataaaa aagagcgtgg attgggtaca
    50881 atcccgctct tatcaccgca ttttgactag ctcaataaaa gaaatcagac catgtttgaa
    50941 attaatccgg taaataatcg cattcaggac ctcacggaac gctccgacgt tcttaggggg
    51001 tatctttgac tacgacgcca agaaagagcg tctggaagaa gtaaacgccg agctggaaca
    51061 gccggatgtc tggaacgaac ccgaacgcgc acaggcgctg ggtaaagagc gttcctccct
    51121 cgaagccgtt gtcgacaccc tcgaccaaat gaaacagggg ctggaagatg tttctggtct
    51181 gctggaactg gctgtagaag ctgacgacga agaaaccttt aacgaagccg ttgctgaact
    51241 cgacgccctg gaagaaaaac tggcgcagct tgagttccgc cgtatgttct ctggcgaata
    51301 tgacagcgcc gactgctacc tcgatattca ggcggggtct ggcggtacgg aagcacagga
    51361 ctgggcgagc atgcttgagc gtatgtattt gcgctgggca gaatcgcgtg gtttcaaaac
    51421 tgaaatcatc gaagagtcgg aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat
    51481 ctccggcgat tacgcttacg gctggctgcg tacagaaact ggcgttcacc gcctggtgcg
    51541 taagagcccg tttgactccg gcggtcgtcg ccacacgtcg ttcagctccg cgtttgttta
    51601 tccggaagtt gatgatgata ttgatatcga aatcaacccg gcggatctgc gcattgacgt
    51661 ttatcgcgcg tccggcgcgg gcggtcagca cgttaaccgt accgaatctg cggtgcgtat
    51721 tacccacatc ccgaccggga tcgtgaccca gtgccagaac gaccgttccc agcacaagaa
    51781 caaagaccag gccatgaagc agatgaaagc gaagctttat gaactggaga tgcagaagaa
    51841 aaatgccgag aaacaggcga tggaagataa caaatccgac atcggctggg gcagccagat
    51901 tcgttcttat gtccttgatg actcccgcat taaagatctg cgcaccgggg ttgaaacccg
    51961 caatacgcag gccgtgctgg acggcagcct ggatcaattt atcgaagcaa gtttgaaagc
    52021 agggttatga ggaaccaaca tgtctgaaca acacgcacag ggcgctgacg cggtagtcga
    52081 tcttaacaat gaactgaaaa cgcgtcgtga gaagctggcg aacctgcgtg agcagggaat
    52141 tgccttcccg aacgatttcc gtcgcgatca tacctctgac caattgcacg cagaattcga
    52201 cggtaaagag aacgaagaac ttgaagcgct gaacatcgaa gtcgccgttg ctggccgcat
    52261 gatgacccgc cgtattatgg gtaaagcctc ttttgttacc ctgcaggacg ttggcggtcg
    52321 tattcagctg tacgttgccc gtgacgatct tccggaaggc gtttacaacg agcagttcaa
    52381 aaaatgggac ctcggcgaca tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg
    52441 cgaactgtct atccactgta ccgagttgcg tctgctgacc aaagcactgc gtccgctgcc
    52501 ggataaattc cacggcttgc aggatcagga agcgcgctat cgtcagcgtt atctcgatct
    52561 catctccaac gatgaatccc gcaacacctt taaagtgcgc tcgcagctcc tctctggtat
    52621 tcgccagttc atggtgaacc gcggctttat ggaagttgaa acgccgatga tgcaggtgat
    52681 ccctggcggt gccgctgcgc gtccgtttat cacccaccat aacgcgctgg atctcgacat
    52741 gtacctgcgt atcgcgccgg aactgtacct caagcgtctg gtggttggtg gcttcgagcg
    52801 tgtattcgaa atcaaccgta acttccgtaa cgaaggtatt tccgtacgtc ataacccaga
    52861 gttcaccatg atggaactct atatggctta cgcagattac aaagatctga tcgagctgac
    52921 cgaatcgctg ttccgtactc tggcacagga tattctcggt aagacggaag tgacctacgg
    52981 cgacgtgacg ctggatttcg gtaaaccgtt cgaaaaactg accatgcgtg aagcgattaa
    53041 gaaatatcgc ccggaaaccg acatggcgga tctggacaac ttcgactctg caaaagcgat
    53101 tgttgaatct atcggtatcc acgttgagaa gagctggggt ctgggccgta tcgttaccga
    53161 gatcttcgaa gaagtggcag aagcacatct gattcagccg accttcatta ctgaatatcc
    53221 ggcagaagtt tctccgctgg cgcgtcgtaa cgacgttaac ccggaaatca cagaccgctt
    53281 tgagttcttc attggtggtc gtgaaatcgg taacggcttt agcgagctga atgatgcgga
    53341 agatcaggcg caacgcttcc tggatcaggt tgccgcgaaa gatgcaggtg acgacgaagc
    53401 gatgttctac gacgaagatt acgtcaccgc actggaacat ggcttaccgc cgacagcagg
    53461 tctggggatt ggtatcgacc gtatggtaat gctgttcacc aacagccata ccatccgcga
    53521 cgttattctg ttcccggcga tgcgtccggt gaaataagca ttacgttatg cgcataaccc
    53581 cggcagatgt cggggttttt taattgtgct gcgcgaaagc agacaacaat tttcttgctt
    53641 cactattggc tgcctgcatc accatccacg gactgaacgc ccacggcgtg gcatcaatac
    53701 cgtgtaatac atctgctaaa tcacaccatt gataatccat cacttcatca tcgttgatct
    53761 gtaacgcact ggtggtgcgt gcggcaaata ccggacacac ttcattttcc acaatgccat
    53821 tcggatcggt ggcgcgatag cgaaagtcag gatagataga ttcaggaggc gtaatttcca
    53881 cgccaagctc ataacggcaa cggcggatca ccgcgtcttc gttgctttct cccagttgtg
    53941 ggtgcccaca aaccgagtta gtccacacgc caggccatgc ttttttgctc agtgcgcggc
    54001 gggtaactaa taattgtcct ttggcattaa acagccaact ggagaacgcg agatgtaagc
    54061 gggtgtctgc cgtgtgtgcg gcatactttt ccagcgtacc cgtgggaact ccctgtgcat
    54121 tcaataaaat gacgtgttcc gtttgcataa tttctcacat gtaattctga tcactcatca
    54181 ttatagataa ttgaagagtg aaatgattaa ccaagtgagc gtttatcgac aaccgcccgt
    54241 tttgagcgga tgccgacagg taaaaaccat ttaaaaaagt agcatcatat atttgaagcg
    54301 actttcaatg gccattgcaa cacagtcaga aatgacagga atagagagag taaaaagcaa
    54361 cagcagactg aatatagcgc gcattaaaaa attcatatta ttctccatgc tcatttttct
    54421 tcagcttgcg catcagaagc cacatacgta ctgtcctgac ttttaaatct ttatcagaga
    54481 cggattcctt caccaggtca gcatgatgct gatagccatt aaagaagacg ttcagcacaa
    54541 cggcgctaag ggttgcgagc ataatgccgc tatgtagcag cggttgcagt acggccggta
    54601 acttagaaaa gaaatcgtga gagagcgttg gagtcatgcc aacgccgaga ctgatcgcca
    54661 caatatagag gttgtaacgg ttggtggtgt agttacagcg cgacagaatt cgaatccctg
    54721 tcgccagtac catgccgaac atcaccagac cagcgccgcc cagcacaaat tgcggaatgg
    54781 aggctaccag caccgccatt tttggcacca tgccaaacag gattaatata attcccgaag
    54841 aaatacacac ccagcggcta tgaacgcgcg tcacgctaac caggccaacg ttttgagaaa
    54901 atgacgtgtg ggggaagctg ttaaacgtgc caccaatcat tgtccctacg ccatcgacac
    54961 gcagcccgcg aataatatcg tgcgaagaga gtttacgacc gactatttca cccagtgcca
    55021 ggaacatccc catcgactcg ataaacacga tgattaacac ggcagtcatg gtcagaatgg
    55081 aaacgggatc gaaaatcggc ataccaaacg acatcggggt gacaatcgca aaccacgagg
    55141 catcatgtag cccggataaa ttgacttcat tcatcatcca cgaaagtaaa aagccaaata
    55201 caatccccag taatacggcg acgttggaca taaatccttt cgcatagcga gtaatgagca
    55261 agataaaaat taagacggca aaggagatac ctaaataaac gggattacca tattgcggat
    55321 tccctttacc tccggcggcc cagtcaatac ccacctgaat gatgctaagc ccgatagaag
    55381 taataaccac accggtaacc agtggcggga ataaaggcat caagcgaccg ataagttgcg
    55441 ctaataatgt ggtgataaaa cccgcggcga tagtggcacc aaatataccc agcaggccga
    55501 tatccgggtt catcccaatg gctatcattg gtgttacagc agcaaaggtc accgacataa
    55561 tcaccggcag gcggatcccc ataaagcggc cgataccgat acattgcaat aatgtgacga
    55621 tcccgcagca aaagagatcc gagctaatga gcatcgcaat agcttctttt gagaggccca
    55681 gtcggtcacc aatcataaga ggaacagcga ctgcacctgc gtacatgaca aggacgtgtt
    55741 gcagaccgag tatgatcagc tttcctggtg ataatatgcg gtcaacctca tcagttgggc
    55801 ggtcttgccc agaagatgag ggaagttggg aatctatggc gctcatcagt gtttctcctt
    55861 tcgcccgcca gagtaaaaag caaccgttta cgttaatgaa actgtctaaa cacatgctcg
    55921 tggagtgcca gtttttgcga ggaaggggag ttagagacag gaattgcgga ggcaatcaca
    55981 taatcgtgct taaactacga aaatgacaag agaacctgac aatggaatta tctgtaattt
    56041 tctcatattg atatttcatt aatcattctg gcatgtcgca ctctcgcatt taatcgtttt
    56101 tatctggata gcgctatttg gggcggcaat gttgaaataa tgaaagcggc atagattgag
    56161 gggcaataat ttgaactcag tcgcaaaatt ataattaaaa cggtcatgct acctaatata
    56221 tgcccgttta ttgcatcgcg aaatttgaga agacataact cattaaatga aagggatgca
    56281 aatgaataag tttatcgctg ctgaagccgc ggaatgtata ggctgtcatg cttgtgaaat
    56341 tgcctgtgcg gtggcacaca atcaagaaaa ctggccgctg agtcacagtg actttcgacc
    56401 acgtatccac gttgtaggga aaggcctggc tgcgaatccg gtggcctgcc atcactgcaa
    56461 caatgcccct tgcgttacgg cttgtccggt taatgctctg actttccagt ccgatagcgt
    56521 acaactggac gagcaaaaat gtattggttg taaaagatgc gcaatcgctt gcccctttgg
    56581 cgtcgttgag atggtcgata cgattgcgca gaaatgcgac ctttgtaacc agcgcagttc
    56641 cggcacgcaa gcctgtattg aagtttgccc aacacaggcg ttacgactga tggacgataa
    56701 agggttacag cagataaagg tggcccgcca gcgcaaaacg gcagcaggaa aagcgtcatc
    56761 agacgctcag ccatctcgca gtgcagcgtt gctccccgtt aactcgcgta aaggcgcaga
    56821 taaaatttca gcaagtgaac ggaaaaacca ctttggcgaa atctattgcg ggctggatcc
    56881 acaacaagcg acttatgaga gtgaccgctg tgtttattgt gccgaaaaag ctaactgcaa
    56941 ctggcattgc ccgctgcata acgctattcc ggattacatc cgtctggtac aggagggaaa
    57001 gattattgaa gcggcagaac tttgccacca gaccagttcc ttaccagaaa tctgcggcag
    57061 ggtatgtcca caggaccgtc tttgtgaagg cgcatgtact ttgaaagatc actctggcgc
    57121 agtctctatc ggtaatctgg aacgctacat caccgatacc gcgctggcga tgggctggcg
    57181 tcccgatgtc agcaaagttg ttccccgtat cgagaaagtg gcggtgattg gcgctgggcc
    57241 tgcagggtta gggtgtgctg atattctggc gcgcgcagga gttcaggtcg atgtctttga
    57301 tcgccatcca gaaattggcg gtatgctgac ttttggcatt cctcctttca aactcgataa
    57361 aacggtatta agccagcggc gagagatatt caccgcaatg ggaatcgact ttcatcttaa
    57421 ctgtgaaatt ggccgcgata ttacctttag cgatttaact tctgaatatg atgcagtttt
    57481 catcggcgtg gggacttacg ggatgatgcg agcagatctg ccgcatgaag atgcgcccgg
    57541 tgtcattcag gctctaccgt tcctgactgc ccatacccgc cagctcatgg gattgccgga
    57601 gtctgaagag tatccgctga cggacgtgga aggtaagcga gtcgtggtat tgggcggtgg
    57661 cgatacgaca atggattgtt tgcggacttc cattcgcctc aatgctgcca gcgtgacctg
    57721 cgcgtatcgt cgtgatgaag tcagtatgcc gggctcgcgc aaagaggtgg tcaatgcgcg
    57781 cgaggaaggt gtcgagtttc aattcaatgt tcagccgcaa tatatcgctt gtgacgaaga
    57841 tgggcgttta actgcggtgg gcctgattcg taccgccatg ggtgagccgg ggccggatgg
    57901 tcgccgtcgt cctcgtccgg ttgcgggttc agagtttgaa ttgcccgccg atgttctcat
    57961 tatggccttt ggtttccagg cgcatgccat gccgtggttg cagggcagcg gaattaaact
    58021 cgataaatgg ggcctgattc aaaccggtga cgtcgggtat ttacctaccc agacgcatct
    58081 gaaaaaagtc tttgctggtg gtgatgcagt tcatggcgcg gatctggttg tcactgcaat
    58141 ggccgcagga aggcaggcgg cgcgcgatat gttaactctg tttgatacga aggcatcgtg
    58201 atgaaatcgt taattatcgt taatccggct gactgtattg gctgccgtac ttgtgaagtg
    58261 gcctgtgtgg tcgctcaccc ttcataacag gagttgaatg ccgatatctt tttaccccgg
    58321 ctgaaggtac agcgactgga ctgcatcagt gcgccggtga tgtgccatca gtgtgaaaac
    58381 gccccttgtg ttggcgcttg ccccgtgggg gcgctgacga tgggcgagca ggtggtgcag
    58441 accaattctg cccgttgtat tggctgtcag agttgcgtca gtgcgtgccc gtttggaatg
    58501 atcaccattc agtcgttgcc gggggatacc cggcaacaaa tcgtgaaatg cgatctctgt
    58561 gaacagcggg aagaggggcc tgcctgcgtt gaatcttgcc caacacaggc gctgcagttg
    58621 cttactgaaa gagaactcag gcgagtccgc cagcagcgta ttgttgccag cggtgagaat
    58681 cctctgtgaa agaaatctcc gcgctgaaag gcgcggagaa tctattagat tgcccaacca
    58741 cccgcgtaaa atgcgaccag tgcggcagta atgatcaccg taccaatatt cagcttttgc
    58801 cattcgcgtg caaagacgcg gcctacgacc agtgtcacaa agcccagcat aataccggta
    58861 acgatattac aagtcagaac gatgaacacg gcacacacca ggccagccat agcgtcaata
    58921 aaatcattga aatccagctt cgagacgtta cttaacatca gcaaacctac gtacatcaga
    58981 gcgggtgcag tggcgtaacc agggatcaaa aatgacagcg gtgataagaa cagaatcagc
    59041 aggaataacg cccccactac ggttgcggtt aaccctgttt tacccccggc ggcggttcct
    59101 gccgctgatt cgatataaac cgctgcgggc gctgcgccca ccaggccgga gaatattgaa
    59161 cttactgagt cactggtcag ggctttgccg ccgttgatga tctggttgtc tttatccaac
    59221 aaattcgcct gaccggcgac ggcacggatg gtgccagtag cgtcgaacac tgcggtcatc
    59281 accaatgcca gtacactcgg aagtacagtt ggctggagtg cgcccataat atcgaggctg
    59341 aaaatcagag acttaccatc ttcgccagtc aggcttggca tcgccaccag accgtggtat
    59401 ttcaccgctg ggtcaaagat taagccgatg atcgaaattg caataatcac caacaagata
    59461 ccgccgggta cgcgacactt ctccagaccg aagatgaccg ccagccccag caagctcatc
    59521 atcaccggga aggaggtaaa cgcaccgagc gccactggca agccttcaat cgggtttttg
    59581 ataaccatac ccacaccgtt agcagcaatc agcagcagaa acagcccgat accgatacct
    59641 gtaccgtgag cgatacccat cggcaaatta cgtaagatcc aggtacgcac accggttacg
    59701 gaaatggcgg tgaagatgac gcccatcaga aataccgcgc ccagtgcgac aggaacgcta
    59761 atttgttgcc cgagtaccag actgaatgcg gtaaacgccg tcaaggaaat cgcgcaacca
    59821 atcgccattg gcaaattagc ccataacccc atcagcaacg agccgaagcc cgcgaccaga
    59881 caggtggcaa caaacacagc tgcgggagga aaacctgctt tgcccagcat tcccggaacg
    59941 acgataacgg aataaaccat ggccagaaag gtcgttaagc cagccagtac ttcctgacga
    60001 acggtactgc cacgagcggt aattttaaaa taattatcca gcgcgccctg tggctttggt
    60061 gcgtccggtg tttgtaggat gtctccagac ataatgatgt cctcacgagg atgtaaaagt
    60121 tttattatta gttgcgttcg tacaccagac gaccatcaac gtaggtgcgg tagatcgaac
    60181 ggtcatcgcc caacgtcatc atcacgaaca atttgtcgac taaagaaaca gagttgtcat
    60241 agcgcagctg ctgtagcgga gtggcggtgg gttccatcac cacgaaatca gcctctttgc
    60301 caggtaaaaa gttgccaatc aaatcgtcaa ggcccagaga tttcgctccg ccgagcgtgg
    60361 ccaggtaaaa cgcttcatat gccgagaggc gatagccttg taattgcaac actttgtagg
    60421 cttcgttcag cgtttgcagc atgttgaaag tggttccggc accgatatcc gttcccatgc
    60481 ccactttaac tttcttctgc catgcttttt tcaagttgaa taacccgctg ccgaggtaaa
    60541 ggttggaggt cggacagaaa gcaatgctgg atttggtttc gctgagacga tcccactctt
    60601 tttctttgag atggacgcag tgagcaaaga cacagttttt accggtcagg ccgtactgat
    60661 ggtaaacatc cagataacca tcatggtcag gataaagcga tttcacccag gcaatttcat
    60721 ctttgttttc acagagatgg gtatgtaccc acgtatccgg atattcctct ttcaggcgtt
    60781 gcgccatcgc catctgttca ggagaagaag taggggcgaa gcgtggcgta atcgcatata
    60841 gcagacgacc atttttgtgc cagcgttcaa tcagttcttt gctttggtga tagctgcttt
    60901 cggcagtgtc tagcagataa tccggtgcgt tgcggtccat catcacctta ccggcaatca
    60961 tacgcatatt gatatgactg gcggcttcaa acagcgcatc aacggattgc ggatgaacag
    61021 tgccaaacac cagcgccgtg gtggttccgt tacgtaaaag ctgcttgatg aagaacgccg
    61081 acatttcgcg ggcgtactct aaatcctcat aacgacgttc agtagggaag gtgtgtttat
    61141 tcaaccactc cagcaattgt tccccatagg cccccaccat ttcactttgc ggataatgga
    61201 tatgtgtatc gacaaagccc ggtactatca gtttgccgcg atagtcgcgc acgcgaatag
    61261 tgtcaggaat ttgatgcttt ccgtcttccc attcgccaaa ccattccact tttccctgtt
    61321 taatgagtaa taaaccatcc tcaataaacc gcagcgcaga ggcaatctct tccgggttat
    61381 cgaccgtacg ggtgacatca ataaaactgc ctcgtaccgc ttttaacgtg tgttctcctg
    61441 acatcattga ctcctttact gttaatccat ctcttccgct gaggtaatac caggcagaac
    61501 gttttcctgt cggtagccac cagggaggat aatatttaat aaaatcgcag ttaacccgcc
    61561 agcacaaata gggttttcaa ctaatacata aatagaggct ggcaatattt taaaaatttc
    61621 gggatcgtag gagacgccaa gtcctaaacc taaagaagtg gcgacaataa gtgtttctcg
    61681 gcgctttaaa ccgttggtga tgatgatgcg aatcccagcg atggcaatca tggaaaacat
    61741 caacgtcatt gcgcctccca gaactgccga gggaatggtc gtgaagaagc cgccaatcat
    61801 cggaaacaaa ccgaggataa ccagcattac cgcgatggtt cgcccgacat aacgtgaagc
    61861 gacgccagtc atctgaataa ccccattatt ttgcgcaaac gtggttaagg gtaatgaccc
    61921 gacagcggag gcgataacag aaaccagacc gtctgccagc acgccgcctt tcagtcggga
    61981 ctgatactct tctccctgaa tgggtcggcg ggaaaccatt gccgtggcgg tgatatcgcc
    62041 gacagcttcc agcacgctaa gcagataaat cgtgccaacc accaggaact gatggaagct
    62101 aaaactaaag ccgtatttga acggatgtgg gatggtgatt aacggcaaat tgcgcatact
    62161 gctgaaatcc accatgccca ggcataacga cgcgatataa ccgacgcata gcccaatggc
    62221 gatccctccc atgcgtagca acggactgcg acagcagtta aagccgatca ccacaatcaa
    62281 aaccaataaa ccaacgccga gatgttcgta attgccgaag gtaccgctgc ttttggcagc
    62341 aaatcctcca ccaaaatcga taatgccgac tttaatcagg cttaagccga tcatcagtac
    62401 cacaataccg ctgacagtag gcgtaataac ccgacgtaaa tagggcaaga taaatgaaga
    62461 tccgacaacc agaaatgcgc caacgaagga gacgccgagg agcgacgaca tgattaactc
    62521 ttcgtgaaaa ccgtcgcttt tcatgctgct gcccagcgca atcatgaccg taacaaatga
    62581 aaaattgact gactgaattg agagtaggcc agaaccgacg atgccatagc ggtttacttg
    62641 taaccaggta ccaataccag aggcgatcat cgccattgaa acaagatagg cagttgtttc
    62701 agcggaaagc tgtaaggccg cccccacgat taacgcgggg gttaccatcg gaacgaaaat
    62761 tgccaacaga tgggtaatgg caccaacgag agcctgatga aagggagggc gatcctccag
    62821 ttcaaatata aggtcagaac ctgcatggtt tatatcagac atcccttcac tccttttaaa
    62881 gtaaatttga tcatctgaat tagtaaaata aattcccacg aatttggggc gcaggacgcc
    62941 ttgagaacgg cttatctcat tttcgcagtc actatttagt catcgtccaa acagagaaac
    63001 aggccgccga agcggccatt tattatttat atcttttcca gcgcggtgag tattttctcc
    63061 ggtgtgaaat gccattcgcg taaccagatg ccgcatgcat cgtgaattgc ggtagcaatc
    63121 gccggagctg cgccatttac accgatttcc gagatcgatt tcgccccgaa cgggccgact
    63181 ttatcgtcgc tcggcaccag cactgcgcgg aaatcacgcg gaatgtcacc aattttcggt
    63241 gcgccgtagc tgcgtaaatc acgcgttaac gggtgacctt cggcgtcata aatgatctct
    63301 tcgctcatac tgtggccgat agctcgcagg gtagcaccgt agatttgtcc caacgctaac
    63361 tctggattga ccggtgtacc gcagtccagc aaggcgtaga atttatccag gcggatttca
    63421 cccgtacgcg tgttgacggc aacttcagcg aagtttgcgc catacgggaa ggcgaaatca
    63481 ggcgtgatat aactgccagt tcccaccagt gaaccaaagc cggtgccggt ttcgccttta
    63541 tgggcaatat ccccgaaact aacttcgcct ttcttgccgc gcacgacgcc cggcgttgct
    63601 agttgaacat ctgccactgg ctcacccaac atttgcgcgc cgtggaacag aattttctcc
    63661 cgcagatttt ccgctgccaa acgcgccgcg ttacccgaga agcaagtacc ggacgaggca
    63721 tatgcgcctt tatcaaacaa cgcatgatcg gtatcaccgg agataacatg cacgtcctgc
    63781 ggtgggcagt gcagcacttc tgctgccagt ttcgtcacca cggtatccag accagtacca
    63841 atatccgcac cgccagaatg aacgataaag gtaccgtctg attccagttt gatcatgcag
    63901 ttagcctgat cgatatccgg gatccctgat ttctgcatga taatggcgac accacgaccg
    63961 atgtgccagt caccattttg tggttttggt gaagaccatt ggatcatctc gcgcccctga
    64021 cgcaggatct cttccagtgc gcagctggcg gcggaaggaa cggaggtcgg cgctttacct
    64081 tcaccgattg caccgagaat tttcagctct tgcccttcgt gtacccggtt acgttcgata
    64141 atttccagtt ggtcgatctg taactgttca gccagttccg ctaatgccat ggtgatagcg
    64201 aagttacctt tcggtgcgcc ataaccctga taagcaccat ttgggcaaat gttgctgtag
    64261 taggtggtga cctggaaatc gacgttatcg cacggatata acggcagcga cagcgccggt
    64321 ccgttacacg gtacggtgag tgagtggttg ccgtaagggc cagtgttggc gcggaaatcc
    64381 atcttcactg ccgtcaggcg accatctttt ttcgctccca gtttgacggt gactttcgcg
    64441 acgtgacgag aggtgttagc aataaactct tcttcacggg tgtagcggaa cagtaccgga
    64501 cgcccggtca cgcaggttgc ccaggcgcac acttcttcca gcaggatgtc ctgtttggaa
    64561 ccaaaaccgc cgccaactcg ctctttaatg acatgaactt tatgctgttt catgtccacg
    64621 aggcgcgcga cctggcggcg taagtgccat ggtacctggg tggaggcgtg gataaccaga
    64681 cgatcgccgt ccatccgggt aaagcagata tgtgtttcag tcgggcactg ctgcgcctgc
    64741 gttgagttat aggttcgctc aatgatcaca tcggcatcgg caaagccttt gtccatatcg
    64801 ccaatatgac catgaatact agcggcgata tttttgcgag ggcgagaacc gatcgggaag
    64861 ttgatgatca tatgctcgcc gcgctgggct gcatggctgt tatcgtcttc cagagtatct
    64921 ggcgcaccag caacatacac caccggttca tcgtgcacga caggcgcatc ttccgccatt
    64981 gcttcgtcga tcgacattac cggcttaagc acttcatatt caacctcgat gagtttcaat
    65041 gcttcgagcg caatttcttc actttcagcg accaccgcag caacgcgatc gccgacgtga
    65101 cgcatttttt tgccgaacat acggcggtca agcggtgacg gttccggtgc gctctgaccc
    65161 cccggggtat agtagatatc agggcaattc aggtgagtaa taacgtgaac gacgcccggt
    65221 aaggcttcag ctttgctgac atccagatga gtaatcagtg cgtgagcgtg tgggctacgt
    65281 aacattttaa tgacgcaggc gtcagccgtt acgcggtctt caacatagca gggtttcgcc
    65341 tgcaccattt tcgcggcatc agttttagga taatgcttgc caatgacttc aaggtcgtca
    65401 cggaaagtcg gagcgatatc aatggtggcc tgcggattat ttttacgtgc aaccgccagt
    65461 tcaatgacct gatagtattg ctgccagcca gcatcgcggc tgaacaaacc agaaagcgcg
    65521 tcgtcgatct cttcgcgggt aggtgcggcg atgcgatcga gaagatcggt gataatcaga
    65581 gctgcagctg gatcgttata accagactgc accacgccaa catcaaccat tgcctgttga
    65641 accagactta actcgttcca tttgcccaga gattctgcgg tacgaatatc tgccttctct
    65701 aactgtgcgg caataagcaa ggacgcgtta acgatattac cgttaaagat gattgcgtca
    65761 gaaccggcaa acccgaaacc atcatcactg ttgcgtacag agtgcattcc catgttaaac
    65821 aacagctttt gcacgttttc gcctggatta acggttagct cctgaggcgc gccatttaaa
    65881 gtaaagtgga tgatcatacg gcttcctccc cagcctgttg gcagtcggca tacaggtcgg
    65941 ctaccagaac tcccgtgata tagcgtttat aggccacgct gccccgcaaa tcttcctgcg
    66001 ggaaaatggc gttggcgaca gcctgttcaa gtgcattgcc ttccagattt tgtttttcga
    66061 catcatgcag acgcagtgct ttactggcga cgccatccag cgcaattcgc ataccgtcgt
    66121 ggtctgtcat tgcaacggct gccgtcacga cggttaaacc tgcctgagaa cggctaattt
    66181 tgcgggtcgc acaggtgcga tacggatctt taatgataat ttcggttagc aggcgatcgc
    66241 atgggcaggc caggtagtcc tcgattgaca gcgtttcgcc gttgccaaaa accagttcag
    66301 catccagtgc cagcaggacg ggaagcagca ctgactcttc ctgccgggcg gcgatttcac
    66361 caccaatggt cgactgatta cgaacatggc gtgagtaaac aaaaccgagg gcttcacgca
    66421 gtgctgcagg aataaatcgc gcatcacgca gtggctgcaa gcgagacatt gcgccaatcc
    66481 gcagtgcacc gttatcccag tcaacccagt ccagttccag atcctgtaag gaaatggcaa
    66541 tctttttatc ggtacgggtt ggtgtagcat tgagtttgct gcccccggcg aaccagacgg
    66601 cttcatcctg gtagcggcgc ttcagttcca gcgcctgttc gacagagtcg ggcctgaaaa
    66661 attgttcaat catcttcttt ccttatttaa ccctcttcct gcgggaagag ggcatctgtc
    66721 atttatgcca gcgcatccat ccgacgccac atactggcag cggcttttct ggcttgtgca
    66781 taaatggaat cgcaatcgaa gttaaactga cggtcttcat agaccatcac accattgacc
    66841 atcacgctgt gaacgctgcc tgagcccata ccgaaagcga tatgcccggc gatattgtct
    66901 gccagcagcg gcgtcggcga gttgtaatcg caaatggtta aatcagcttt gtaaccggct
    66961 tccagaagcc caaatttcgc gccaaagttg cggctcatca gttcgttacc gttagtcagg
    67021 gctttggcaa aactgtcagg ccacagcgga ccacccgcat cgcgatgttt aaagaaggca
    67081 aatttcatct cttcaaacat gtccgaacca atgccgtccg ttcccaacgc cagattgcgg
    67141 atgtcgctaa ggtgatggtt gtagccgaca tggttgttca tgtttgaacg ggcgttatgc
    67201 accaggaacg catcgcgctg attgagtagg gcgatgtcat ctttcgacaa gtacagccca
    67261 tgagcgacca gcgttttgct atcgatgaga tcgaattgcg ccagtcgtgc cagcaggtct
    67321 ttgccgtacc agtggtgact gtaggaaacg tcgtaaaggt cttccgcagc gtgaatatgc
    67381 aaaccacggc ctgtggcgtt cacggcttca cgtagcatct ccagaccggc atctggcacg
    67441 gtaaacggcg cgtgagcacc gatatgtgct tccaccagat acggctcggt tgctgctttc
    67501 ttcgcctcat caatctgacg ggcgaagcgg atgttttctt ctacaccttc ctgcaactct
    67561 ttgatgccgt tgttacggtc agtagtttca aaacaggtca tcgcgcgcag gccaactttt
    67621 aaaaatgcgt cgcgcaatgt ggagagcgac ccgccgatat acgccggaga ggcatggtga
    67681 tcgataaccg atgtacatcc gctcttaatc gcttccaggg aacaaatcag tccgctgtaa
    67741 tagagcgact cttcatcaag ggcgcgatcg agccgccacc agagattttt cagcgttgag
    67801 atgaaatctg ggctgggggc gatgtttgcc ataattccgc gggaaagccc cgagtaaaaa
    67861 tggttgtgcg agcagacaat tcccggcatc acaatccgac catgcatctc tttgtagctg
    67921 gcatcggggt agcgttgcgt cagggcatcg ccgatagcga caatcacatc attttcgata
    67981 gcgatatcaa cgccttcctg cactttcgcc gggtgtaact gcactgcggt gacattcttc
    68041 agaatcaaca tgattacacc tccacgcggc ccagcagata atgatgatgc tggtggacat
    68101 ggctgatgat gcggcacata tcgttcagct ccggtggtac gttgttgaac tgaccttcgc
    68161 tgtcgatgtt taacacccag ctttggttat tcagtcgtac tcgtacccgg caatcttcca
    68221 caaggaagcc tgggttgctg ctgttatcaa agtcttgcgc caggctgaaa acggtgattt
    68281 tgtctttgta tggtttaccg ttccacggac agaactgagc gcagttgccg cattcgttac
    68341 agtaagcgtc gaggtgcagc gtctggaatc tattctggaa gcctgggacc gcaatggaga
    68401 cgttggcgcg gttcgggcag acatccacac acttgctgca aacgtagtta cattcaaggc
    68461 agcgagcagc ttcctgcgcg acaaacgcat cacgatcgtc actgttcacc agcgtgatag
    68521 agatatcgcc tttacgttga tagatttccg ccggattgac gttgttccag tatttatcgt
    68581 tctggtggga acggatattt tcccgactaa ggatggcatc ggtcgcccga cgcgcggttc
    68641 cgacagcagc gacaatggag gatggtccgc gctgtacgtc gccgatcata aagacgtcag
    68701 tcagacgagt ttcgccatta tggtcgacgt ctggccagcc gtttttgtcc agcggcacgc
    68761 ccatcgcatt cagggcttca gtatcctgct gttcaccaat ggcggtgatc aggctgtcta
    68821 catgcagtgt tactgtttcg ttggtttcaa ccggacgacg acgacctttc tcatcgggtt
    68881 cgccaagcga cataacgcgc aaggttaagg tgccatcagc atcgaaacgt tccggattat
    68941 tcaggaaacg gaactccacg ccgtcgtgca acgcttcttc atactcttcg cgccatgcgg
    69001 gcatctcttg cagtgaacga cggtaaacga ccgttgcttt ttctacgcct ggaacgcgta
    69061 acgccgcacg agcgcagtcc attgcggtgt tacccgcccc gacaacgacc acatgtttgc
    69121 ccagcttgag cgctgtaccc ttgttgtatt cacgcaggaa ggggagtgat ttcaagacat
    69181 tttggttgtc gcccgccagt ttcacaccgc tatttttatc tgtgccggtg gcaatcagaa
    69241 catagtggaa gtcctgattt tttaactgct caacggttaa atcgggtgag cagccatact
    69301 caaatttcac gccgtgagcg gcaacaaaat cgatatcgtg ctgaattaac tcagccggaa
    69361 ttcggaactg aggaatgata tttttcacca cgccgcccgc attggcttcg cgttcaaaca
    69421 gcgtaaccgg atggcccgct ctggcaagga agtaacctgc tgccagaccc gccggacctg
    69481 caccaatcac ggcaaccgga tggcgtgaac cagaaccggc tggtttgtgc cagcgttgct
    69541 tatattcatc ccaacctttt tccagcgcga cttttttcag ttcgcggata ttcagcgcac
    69601 tgtcgtaatc caggcgggta cagttgtatt ggcactggtg atcgcaaata tgaccggtaa
    69661 tggcgggcag agcgttgcgt tggtagatga gttccagcgc gtcggcatag cggtgttcgc
    69721 caagcagacg gatgtattcc ggaatatctt gcttgatagc gcaggcagta acacaggggg
    69781 caacgtagca gtcggtcagc ggcaggtctt ctgccacttc aatacgctct tctggcttcc
    69841 agtgtttctg ggtgtattcc atggttaacg catctgctgc cagtctgttc agtcgttcga
    69901 cgtcaacatg gtcaagtccc caggcgtcgg agccttccag ctcgcgcatg caggcactta
    69961 agcgcaaata gccgccaggt ttcagcaggt cggttgccat agtaataggg cgaatacctg
    70021 tatcaaaaat atcgcggata gtcagctgac tggcaccacc ggaataagaa atgggcagtt
    70081 tgccgtcaaa ggcgcgagag agaactgctg caacattgat ggagagcggg aacagcgcac
    70141 ggcctgacat atacatctct tcaccaggca gtgcgccttt attgttgatg gtgccgagag
    70201 tgttagtcag ttttacgcca aagccgagtg atttttcttt tgccagtgcc atcaggcgtt
    70261 ccagcatttc cagtgcttgc gtcagcttga ggtcgtgatc aaatgactct tcttttaagc
    70321 ctatgtaacc gaaaccgcag acatcgagga tctcacgaac acgcgcgtac cccagtaagg
    70381 tcgggttaag tttcacaaag gtgttgagcc ctttttcttc cagcatgtag cggcaaatgg
    70441 cttcgatttc atgcggagga cagccgtgca tggtggagag ggtgacgcca tgcaccatac
    70501 tggtggggat gcgagcgggt aaggcttgca agctttcgcg tttttcctgc aatccgtggc
    70561 gggctaaaaa tgcgtcatcc tggagtaatt tattcagcgt atcgcgatat tgcgcgaatt
    70621 tcggatggtc agatgcgtcc atcatattgt cgatgaactg ttgcattggc ggttgcttaa
    70681 taccttcgag gttgtaaccg acgctcatat taaagatgaa cgatttaccg gaatcagaag
    70741 gttggaacat cgcttcgaga aggtgcaggg caaaccaggc tttgaggtat tcatcccagg
    70801 ctttaagcag ggtaaattcg gtagaccatt cggtgttaaa gcactcgtct tcggcatcaa
    70861 tacagggctt ttccagctcc aggcggtcaa gaatttggac ggtttttagt tcgatgaaac
    70921 gtccgccagt cagccaggaa gtgacaatat tttgcgcgag ctgcgtgtgc gggccagcgg
    70981 cagggccgac gggagtggca caggtttcac cgaagacgct aacagtttta ccttttacag
    71041 gtgagtaaaa ctgttgctcg ggaataccaa agattgagcg ttgttgttgg tattcatcaa
    71101 atatgcgcgt caaaagttcc tcaaacggaa tgggacgcat aatatccccc ataaccctct
    71161 ccttagctta tacagatatc attgagtgac cggtgttgag aaatccggtg gtttaggggg
    71221 atagagcaac aatcgcacca ctcttgccag gtttggcggg aaatataaaa aaatgtgagg
    71281 cggctcgaaa cttaagggct ttttgtgaat gaactcacag gtgctaaatg agaattaaaa
    71341 tcactcctga aaattattat tgccatccca tatatcactt tttctctttt ctcaaaatca
    71401 gtgattcctt atcattatga taaagagtgc tggagggatt aaatatttga acgcaatcgt
    71461 ttaactacgt tttttaaatt ttagtatacc ttttttgcgc tgtaataaag tcgtccggag
    71521 tatcaatatc taaaattatt tctgcatttt ctacttcgac ggaataatga gctcccataa
    71581 ggagtgcctg acgcatattg gtgatattgg ggcgcttgat tgcctgcatc aggcatggtt
    71641 ttgacactaa aatcggatgg ccggggatgc cattatggag tggcagtatt gcgccatcat
    71701 ttcgtaacga ccagattttt ctgaaaatat cgatggtgag ggttggcatg tcaccgtggg
    71761 tgagaaaaca atgttctgtt tgcactgcgg gtactgcggc ctttactgat gttagtaaac
    71821 cctgcgcata atctgggttg tggataatag tgatattgct ctggttcgca taacgttcgt
    71881 gcagttcatt accacgatag ccggtgacta aaataattcg gctacaaaac tgcaacgcat
    71941 ttttgatact tgtatcaaga attgtcccct gttgccaggg taacatcatt ttccattgcc
    72001 ccattcttga tgataatccg gcagcggtaa ttatacagtc gatggctgac atagtggttc
    72061 cattatttat aggggctaat aacgcctgat aattgcgtta cttcctgatg gattaagttg
    72121 gggataattt atcccagaga ggtcataaag actcgtgaaa agtatagttg acccatcggc
    72181 attattcatt gatttaggtg cgcagaaacg tcccactgtg atttctgttg ttggtgctgg
    72241 ggggaaaacc agcttgcttt tttggctggc agaactgctc caggctagcg gcaggcgtgt
    72301 gttaatcacg acgacaacac atatgtttat gccaacgtct cactggcccg tggttttctg
    72361 tcgtgatccc gccatgcttc ctcatgcgtc tcttacatcc cccatttcat tttgttttca
    72421 cagctggaaa gcgaacctgg gaaaagtgca gggatttacg ccagaagcga ttgatgcact
    72481 ggtgcaacga ccagagtgtg acgtaattct cattgaagca gatggctcgc gtgcaatgcc
    72541 gttaaaagcg cctgatgagc acgaaccttg catacccaaa agcagttgct gcgtgattgc
    72601 tgtgatggga ggacatattt tgggcgcgaa agtgagcacg gaaaatgtcc atcgctggtc
    72661 gcagtttgct gatattaccg ggttaacacc tgatgcaacc ttgcaactga gcgatctcgt
    72721 tgcgctggtt cgccaccctc agggggcgtt taaaaacgta ccgcaaggtt gtcggcgagt
    72781 ctggttcatt aaccgttttt ctcaatgtga gaatgcgatt gcgcaaagcg agctccttca
    72841 accgctgcaa caacacgacg tagaggcaat ctggctgggc gatatacaag aacatcctgc
    72901 aatcgcgcgc agatttgtga attagcggcc cgggtattcc gggttactga aaggatatcc
    72961 attgaggttg gtatgaatat tttcacagag gctgcaaaac tcgaagagca aaattgtccg
    73021 tttgctatgg cgcaaattat tgatagccga ggctcgactc cccgccattc tgcacaaatg
    73081 ttagtgcgcg ccgatggctc tatcgtcggt acaattggtg gcggaatggt tgagcggaag
    73141 gtgattgaag agtcgcttca ggcattgcag gaacgtaagc cgcgattatt ccatggacgt
    73201 atggctcgta acggtgcgga tgctgtcggg tcagattgtg gaggtgcgat gtcagtgttt
    73261 atcagcgtcc atggtatgcg tccacgtctg gtgttgattg gcgcggggca tgtcaaccgg
    73321 gcgatagccc agagtgcggc gctattagga tttgatattg ccgttgccga tatttatcgc
    73381 gaaagcctca atcctgaact attccctcca tcaaccacgc ttctccatgc tgagtcgttt
    73441 ggtgcggcag tggaagcact ggatattcgc cctgataatt ttgtcctgat tgccacgaat
    73501 aatcaggatc gtgaagccct cgacaaactc attgaacagc ccattgcatg gttggggttg
    73561 ctggcaagtc gtcgcaaggt tcagcttttc ctgcgtcaat tgcgtgagaa aggcgtggct
    73621 gaagaacata ttgcccgttt acatgcgccc gttggttaca acataggtgc ggaaacgccg
    73681 caggagatcg ccatcagcgt gctggcagaa atattacagg tgaaaaataa cgcgccgggt
    73741 gggctgatga tgaaaccttc tcatccttcc ggacaccagc tggtggtgat tcgcggtgcg
    73801 ggagatatcg ccagtggtgt ggcgctacgt ctgtatcatg cgggttttaa agtgatcatg
    73861 ctggaagtgg aaaaaccgac agtgattcgt tgtaccgtgg cgtttgccca ggccgtgttc
    73921 gatggcgaaa tgacggtcga aggcgtcact gctcgcctgg caaccagctc tgcggaagcg
    73981 atgaaactta ccgaacgcgg attcatccct gtgatggtag atcccgcctg ttcattgctt
    74041 gatgaactga aaccgctttg cgtggtggac gctattctgg cgaaacagaa tttgggaaca
    74101 cgggcagata tggcaccagt aacaatcgcg cttgggccgg gctttgctgc agggaaggat
    74161 tgtcatgcgg taattgaaac aaatcgcggg cactggctcg gtcaggtgat ttactgtggt
    74221 tgtgcgcagg agaataccgg tgttcctggc aatattatgg ggcataccac ccgacgggtg
    74281 atccgtgctc ctgctgcagg cattatgcga tccaacgtga aattaggcga tctggtgaaa
    74341 gagggcgatg tgattgcctg gattggtgag catgaaatta aagcaccgtt gacggggatg
    74401 gtgcgtggct tgttgaacga cggcctggca gtggttggtg gttttaaaat tggtgatatc
    74461 gatcctcgtg gtgaaaccgc tgatttcacc agcgtttctg ataaagcccg ggcgattggc
    74521 ggcggcgtac ttgaggcgtt aatgatgttg atgcatcagg gcgtgaaagc gacaaaagaa
    74581 gtgctggaag tggcttaaat gaaaatggcc ggatggtatt acccacccgg ccttaaaatt
    74641 actgacaaat caccgttccg gttttgcctt caatcccttc tttcgctttg ctcaatacgg
    74701 taatcagtgc ttcacgacct gcgcgagagc gagcaaacga ggcggcagct tccactttag
    74761 gcagcataga acctttcgca aaatgacctt cttcgataaa gcgttcagca tcacttaatg
    74821 acaggcgatc gagccactgt tcattctctt taccgaagtt aatggccact ttttctaccg
    74881 cagtcagaat gatcagcata tcggcatcaa tcatttctgc taaacgggcg ctggcccagt
    74941 ctttatcgat aaccgcgctg gcaccacgca gatggttacc ttcacgaata acaggaatac
    75001 caccaccgcc aacggtgatc accacctggc ctgcttctac cagcgctttt accgtctctt
    75061 tttcaatgat atcaaccggt tttggcgagg caacaacacg gcgatagccg cgaccagcat
    75121 cttctttcag agtgtaaccc tgttttgtca gttgctctgc ttcttgctcg gtaaagaacg
    75181 aaccgatcgg cttggtcggg ttgaggaatg ccggatcgtt agcgtcgact tcgacctgag
    75241 taaccagcgt tactacaggt ttattaatgc cccgagaaag cagttcttct cttaaggcgt
    75301 tttgtagatc gtaaccaata taaccctggc ttaaggcaac acagacagac atcggcagca
    75361 ttggcgaatg cgcttcagtt tttgcggcag cttcaaaggc ctggttaatc atgcccacct
    75421 gcggcccgtt accatgagtg acgataactt catgtccctg ggcaattaaa tcaacaattg
    75481 cctgagaagt aatttttacc gctttcattt gtccggccag gtcatcgccc agcgcattcc
    75541 cgcccagggc gagaacaatt ttcttactca tttttcctca ctcatcattc ttgattgtgt
    75601 atagatccct ggcagtggtt agagcacggg agggacaaac ggtttgcgtc gcaggaaacg
    75661 acctcgcccg gctttgcctg taaaggtgcc gtcacagaaa atcgtttcac cacgggataa
    75721 ggttctgaca atcgcgccct gacaggtaaa accctcccat ggcgagtagt cggcgttgtc
    75781 gtggagatgg cgatgctgaa tttgttggct ctgacgtggg tcgataatca ccacgtcgcc
    75841 atcggaaccg ggcgctaata atcctttttg cggccacagg ccaaacagcc tggcgggcat
    75901 tgcgctggtt aattcaacaa agcgttccgg tgttatacgt cccgtcatca cgccactgga
    75961 aaacagtaac tgcatgcggt tttccacacc gggtaagcca tttgggcagc gactgaaatc
    76021 gcctttagaa atttgcaggc gttgagccat cgagaaggtg cagtgatcgg tcgccaccac
    76081 gtcaatcgca ccatcgctga tgccacacca cagtttgtcc tgctcgcgta cgttacgcag
    76141 cggtgggcta agaatgaact tcatgccgtc ttctgtatcg taactgcgtt cgtccaacag
    76201 gagatattgt gggcaggttt caacccagac tggctggtga ttcgcacggg caagacgcag
    76261 ataatccaga cctaagccgt tagacaggtg cacgatatag agcggggcgt taccggcaat
    76321 ttgtgccagg ttaatcatgc gggcgatggc ttccgcttcg cattccagag ggcgactcaa
    76381 ggcatgatag cgcggcgcgg ttaaccctgc ggcgataaat tccgcccgct tgctggcgat
    76441 agccgcatca ttttccgggt gcacggtggt cagcgcgccg gattcatgca gacggcgtaa
    76501 tgcctgcaaa acctcgtcat cgttgagttt gtattgatag gttaaataga gtttaaaact
    76561 gctcagccct tcctcgacca tcatcgggat ttcgtcgagg atggcgtgat taatgtgctg
    76621 gatcacaccg tgaaagctgt aatcgatgac cgctttatgg gcggcataac cacgataaac
    76681 ctccagttga tggcgtaacc gacagccgtt tgggccaaat cccatatggt caataatggt
    76741 tgttgtaccg ccacacgcag ctgcgcgggt accggtaaaa aaatcatcac aactgcgcgc
    76801 gatgccgaca tcaatattga aatgcgtatg gacatccacg ccgccaggga aaacgtaaca
    76861 gccagtggca tcaatttctt catacgggag ctgcggcgaa atattgttgc ccaactggcg
    76921 aacaatcccg ctttcaatca gcaaatcctg tttggcttgt ccatctgcgt taacgacagt
    76981 gccgtttttg atcaatacgc gcatagcaaa ctccaaaaac cctccggcga accggagggg
    77041 aataagtgaa gagtaattat tcggttgcca gccagcttaa cgggattgca gcgtacatcg
    77101 cggcacaggt caccaggtga gatttccagg ttttttcgtt aggtgcatgc gcttcaggtt
    77161 ctttacccgg gccaaagccg atcaccggaa tgccgtgacg gcccatgata gatacgccgt
    77221 tagttgagaa ggtccactta tcaacaaccg gcgctttgcc aaacagccct tcgtaggcat
    77281 tcaccagtgc tttaacggtg aagtgatctt cttccacttt ccaggtcggg aagtagcatt
    77341 cggttgggta aaccaggcca gtccaggacg gacggtcgta gttgtacata gaaacaaccg
    77401 cgttagcttt ctgtactgca ggcagggcgc ggatttcgtc cagcgcgcct tcccaggttt
    77461 cgccccaggt cagacggcgg tcaatagaga cggcgcagct atctgctaca gcgcaacggc
    77521 ttggggatgt gaagaagatt tcagaaacgg tgagggtgcc tttgccgagg aattcgtcat
    77581 aacccagacg ttgggagagt tcttgtaatt cgccaagaat cggacccatt ttgaaaatgg
    77641 cgttgtcacc gcgttctggc gcagaaccgt ggcagctaac accctgaaca tcaatacgaa
    77701 tttccatacg accgcgctga ccacggtata cctggcagtc ggttggttcg gtactgacca
    77761 caaattccgg gcgaatgccg gattgttcaa taatgtactg ccagcacaga ccgtcgcagt
    77821 cttcttcctg cacagtaccg gtaaccagca gggtatattc atcttccaga ccgaggtctt
    77881 taatgatttt accggcataa accatagatg ccatgccgcc ttcctggtcg gaagtaccgc
    77941 gaccaccgat cagctcatca gtttccatgc cttcgtacgg atcgaagtcc cagtttttga
    78001 tgttgccaat gccgacggta tcgatatgag cgtccattgc caccagacgc gggccgtggc
    78061 cgatataacc gagaacgtta cccatcgggt cgatttcaac tttatcgaag ccgacttttt
    78121 ccatctcttc tttaatacga tgtactaccc gtttctcgtc gcagctttca ctgggaatag
    78181 caaccatgtc gcgcaggaag cgagtcatat ccgcctggta atcttttgct ttttcaagaa
    78241 tcagtttgaa tggaatattc ttagccatta tttttctcca gattttttac tccccgtttt
    78301 gcagggagca gttccaatag ggtgattaag gtgctacagc gtgtttgcct tcccagacaa
    78361 cttcgcggta gtgcttcacg tcggtgtcgc cttcagtgct gataaccagc actacggcat
    78421 ctttgttcag cgccagtttt tccatcaggc tttgacgttg cgggtgataa tgaaccgctg
    78481 cgagaacgcc caaaccgaca gcgccggatt caccggagat gatgcgcggg tcgttgccgt
    78541 acggattacc cagcacgcgc atacctaatg cggcaacgct gtcctggcag gagatgaatt
    78601 gggtggcgca gttacgtagg atttcccagc ccagcgggtt aggttcgcca caggccaggc
    78661 ctgccatgat ggtggccata tcaccgccaa cgttgacgat gtcgcctttg acgccggagc
    78721 gataaataca gtcagctttg tcaggttcaa caataatgct gtgcagattt tgcgggctat
    78781 agacgtcgac cagataaccc agcacaccac cggccattgc tccgacaccg gcttgcagca
    78841 gaacgtgcgt cggggttacg cccatttcac gcatttgctc gacggcttca tctgccaggg
    78901 ttgcgtagcc ttgcatgatc caggttggga ttttggtgta accttcccat gccgtgtcct
    78961 gtaccacttc ccagccgtgc tgctgcgcgt gttgcatggt caggcgaacg gtatcgtcat
    79021 agttcatatc tgtgacgatg cactcggcac cgaggttcag aatggcgtca acgcgttcct
    79081 gagcagaacc tttcggcatg taaatcaccg cattctgtcc gagttgctgc gctgcccacg
    79141 ccacaccgcg cccgtggttg ccgtcggtgg tggtcgcgaa agtcattttt tcgccgatgg
    79201 catttttcag gtgctcaaat gacagcgttt cgatatcaag atgatatttt tcacacaata
    79261 attgagcgat agcgtacgca ccgccgagca ttttgaaggc gttcagaccg aatcgttttg
    79321 attcgtcttt gacaagaatt ttcttcaccc caaaaaggtt tgcgagatcg tccagcgcac
    79381 aaagcggtgt tgggcgataa ccagctattt tctggtggaa ctggcgcgcc agtttggcct
    79441 ggctttgcga aaagagcggt gatgtttcgc cgttgaaaaa tttgttatcg gcgatatcaa
    79501 tcttcaatga gaaaacggac atatcctatc cttttggcct ctggaaatag agggccgacc
    79561 agctgatcgg cccgaggggt tatttcacgc gttcttgcgc ttctttcagc agttgctcga
    79621 gcagtgcacc tggtttggcg tatttacggg acaggatcat cgcggcgatg atataaggct
    79681 tccagctggc ttctttgtag gtagcgatac ggtatttttc gaatacgcct tcagtcactt
    79741 ccccttcttt gcaggatacg ccgctgatat cagctggcag gcagtgcatg tacagggctt
    79801 cgccatcacg ggtcagttcc atcatctctt cagtacaatg ccagtctttg tgttgcgcgt
    79861 tctgtgccag acactgtttt tccagtgctt ttaagccttc gtgatcgtta gcgcgcagca
    79921 attcggtacg ctgttccatc actttgtaag gtgcccatga cttcggataa acgatgtctg
    79981 catctttgaa cgcttcttcc atgctggtga cctgacggaa gctaccgcca gatgctttag
    80041 cattgttttt cgcgacttct actacatccg ggatcaggtc gtagccttcc ggatgggcca
    80101 gggtgacatc cataccgaag cgagtcatca gaccgatgat gccttgtggt acagagagcg
    80161 gtttgccata gcttggagag taggcccagg tcatggcgat ttttttacct ttcaggtttt
    80221 ccagtgaacc aaagtgttca cgtaaccatg ccaggtcagc cattgactga gtcgggtggt
    80281 caatatcgca ttgcaggttc actaaagccg gacgctgtgg cagtacaccc tgcttgtaac
    80341 cgtcatcaag tgcagcgcca acttcacgca tataggcgtt gcctgcgccc agatacatat
    80401 cgtcgcgaat accaatagcg tcggcgcaga aggagatcat attggcggtt tcacgcacgg
    80461 tttcgccgtg agcgatttgt gatttgcctt catcgagatc ttgctgtgca aggccgagca
    80521 ggttaagcgc ggaagcataa gagaagcggg tacgagtgga gttgtcgcgg aatacggaaa
    80581 tacctaatcc actattaaag actttggttg agatgttttc agcacgcagt gcttttaatg
    80641 ctgcggcaac gtccagtact tgtttcagtt catctggagt ctgttcccac gttaacaaaa
    80701 aatctttctc gtgaaggtga gaggtcagcg aattgatatc cttaatcagc tcattaacag
    80761 ttttcatcat aaagtcccgg tagtggaatg acaggcaaag tggtttattt cactgtgact
    80821 tgccaaagtg attggtgttt tcacagctat acctgggact aacaataagt gtgccagaat
    80881 tatccacggg gctgcaaaag actaatttga cggataatgt gtggggttga tcacaaattg
    80941 atgccagctt ccggttgttt gtaaaatacg aaaacgtttg gcttttttta tcgcgttatt
    81001 tatttgctgc taagtatcag aaataagata aagggacagg ctatccctcg tcagtttgat
    81061 aaactcaccg ccataattct catattgata tagaaatgtg atatctcttc cataatttca
    81121 ttagaaagtg ataaaaatct tatgttttta tcaaagccaa tttcgcctga gttaatatga
    81181 tcatgatctg tgaaccatca acgtcttcaa tgatcgactt ttaagtaagt ctggaaggta
    81241 aagctatgga gcttgctact acgcagtcag tattgatgca aattcaaccg acaattcagc
    81301 gttttgccag aatgcttgcc agcgttttgc agcttgaggt tgagatcgtt gatgaaaact
    81361 tgtatcgcgt cgccggaacg ggcgcgtatg ggaagtttct tggtcgccag ttgagcggca
    81421 actcacgcct gctctgccac gtcctggaaa cgaaaactga aaaagttgtg acacagtctc
    81481 gcttcgatcc cctttgcgaa ggttgcgata gtaaagaaaa ttgccgcgaa aaagcatttc
    81541 tgggtacgcc tgtcatttta caggatcgtt gtgttggggt gataagtttg attgccgtta
    81601 cccacgagca acaagagcat atcagtgata atttacgcga attttctgat tatgttcgcc
    81661 atatatccac catttttgtt tcgaaacttc tggaggatca ggggccagga gataacatca
    81721 gtaaaatatt cgcgaccatg atcgataata tggatcaggg cgtattagtt gttgatgatg
    81781 aaagtcgggt tcagtttgtt aatcagactg ccttaaaaac acttggtgtt gtgcaaaata
    81841 atattattgg gaaacctatc cgtttcagac cattaacatt tgagagtaat tttacccatg
    81901 gacatatgca gcatattgtt tcgtgggacg ataaaagtga attaatcatt ggtcaattgc
    81961 ataacattca gggccgacaa ttatttttaa tggcgtttca ccaatcgcat accagttttt
    82021 ctgtagcaaa tgcacctgat gaaccgcata ttgaacaatt ggttggcgag tgccgtgtta
    82081 tgcggcaatt aaaacgactc attagccgta ttgcacccag cccatccagc gttatggtgg
    82141 ttggtgaaag cggcacgggt aaagaagttg tcgcccgtgc aatccataag ttgagcggaa
    82201 gacggaataa accctttatt gctatcaact gtgccgcgat tccggagcag cttctggaga
    82261 gcgaactgtt cggttatgtt aaaggcgcat ttactggcgc ttctgccaac ggtaaaacag
    82321 ggttgattca ggcggcgaat acgggcacgc tgtttctcga tgaaataggt gatatgccat
    82381 taatgttgca ggctaaatta ctgcgcgcta ttgaggcacg tgaaattctg ccgattggtg
    82441 ccagtagccc aatacaagtc gacattcgca tcatttctgc aactaaccag aatttggccc
    82501 agttcattgc cgaaggtaaa ttccgcgaag atctcttcta ccgacttaat gttatcccga
    82561 taactctgcc accgctgcgt gaacgtcagg aagatattga actactggtg cattactttt
    82621 tacatctgca tacccgtcgt ctgggatcgg tttatcctgg cattgctccc gatgtcgtcg
    82681 aaatattgcg taagcatcgt tggcccggaa acctgcgcga gttaagcaat ttgatggaat
    82741 atctggttaa cgttgttcct tcaggtgaag ttatcgacag cacgctattg ccgccaaatc
    82801 tgctgaataa tggcacaacg gagcaaagtg atgtaacaga ggttactgag gcacacctgt
    82861 cactcgatga tgcgggcggc acggcgctgg aggagatgga aaagcaaatg atccgcgagg
    82921 cgctttcacg tcataacagc aagaagcaag ttgctgatga actgggcatc ggcattgcta
    82981 cgctctatcg caagattaag aaatatgagt tgttaaacac ataacatcag tgaatccggc
    83041 ctgaattcag gccggatatc cttttacttc gttttctcgc aatccagaac tgtatttaca
    83101 atcatctgat accccgtgca gcgacaaaga tttcccgcca gtccgcgacg aatttccgta
    83161 atggttaatg gcttctcgcg tggtttcgcc agcattgccg tggtagccat aatcaggcca
    83221 ggcgtacaaa acccgcactg cactgcgccg gatttcgcat aagcctgctg aacatgagaa
    83281 agttttccgc ctttcgcttc accttccagc gtgcggatct cttttccttc agcccaggcg
    83341 gcaaggtata agcaactgtc tattgctgtg ccgtcgacca gcaccgtaca ggcaccacat
    83401 tcacccacgc agcacccttg tttgacactt agcagccctt gttcgcggag taattccgag
    83461 agcggcgtgc ctggtacggc atgaagctga aaaggcatcc cgttaatggt gcattcgatg
    83521 gtaattgttt cgctgtgatt cattgcaatt ttccccccgc cgcggcgacg gcttcgctaa
    83581 tcactttttt ggtcatcgtc tggatgagat gcagacgaaa ctctttactg gcccgccatg
    83641 aagaacgcgg ggcgacatct tgcaggacag attcgctgat agcttccagc gtttgcaggt
    83701 ttaatggcgc attttgtgca gtctgttcgg catgttggca gcgaatcggc gttggcgcgg
    83761 caacaccaaa tgccaggcgt aattcgctga aattgccgtt atccagtcgg caatgtgcgg
    83821 cacagccaat cgttgaaata tccattgcgt cgcgcatggc atatttaaaa tgcgcgctgc
    83881 ccacgtgttc tttcggctgg ggcggaaaat gaaaggcgac aaggatttcg tcatgctcaa
    83941 gagacacttt gcccggcccg gtgtgaaagc cattaatcgg gacgaaacga acaccgcgtg
    84001 gggagtggat ctccagtttc gcgtcataaa tgagcgttgg cgtggcagaa tctgcgctgg
    84061 tggcaccgtt gcaaatattt ccaccgtagg tagcgacgtt acggatctgc ggtccagcaa
    84121 tggacgaggc cgcagcacat aacgccggga gatgacgttg agttatagaa tcttctatta
    84181 gctgggtaaa tgtcgttgca gagccgatac gtagcgagcc atcttccgcc agcgtaattc
    84241 cccgcagctc cgccagatta tgaatatcaa caatatggcg ataacggtca ttgtgatggt
    84301 ggagctgaat cagtacgtca gtgccaccgg cgagcagttt ggcctgcggg ttgtcagcca
    84361 gcaggttgat ggcatcggca agggttgctg cgcgatggta agaagcaaaa tcaaacatga
    84421 tgttatcctc aaatcaatcc tgccagatgg aactcttcat ataaccgttt tggcgtcagc
    84481 ggcagtgtat tgattgcaac accggtagcc atcttcaccg cgttacgaat agcagcggca
    84541 acaggaatta ttggtggctc acccagtgac ttatgtccgt atgcggattg cggctcattg
    84601 atttcgacga acgcgctttc cagttgtggc agatccggca tggtcggcat tttgtaatcc
    84661 agcagattgg ggttacggac cacgccgctt ttcgcatcga tgatcatctc ttcaaatagc
    84721 gcccagccaa tgcccattcc cattccgccg tgtacctgac cttctgccag tagcggatta
    84781 agaatatggc ccgaatcatg aacgttgagg atgcggttga tggtgacttt gcacagcgca
    84841 atatcgaccg tcagatcaac aaaggtacag ccgaacgccg gtgggttagt ggtggttttg
    84901 atggagcttt cagcagagag ctgcccgccg cgttcagggt ggtagaaagc gtccatcgcc
    84961 aaatctttta acgacattaa cggctcttcc ggtcgttcaa ccagcacgat atggcctttt
    85021 atcagggtca gattcatcgc tgactgatgt agcatgactg cggcgtgagc gatgattttc
    85081 tcttttaata acagtgccgc actgcgcagc gcaggcgcgg caacatagct ctggcgtgag
    85141 gcaaatgcgc cgggatcgaa cggcgtaacg tcggtatctt gtgttgaaat aacgcgaacg
    85201 tcgctgaccg gaacccccac ggtttctgcc accatttgcg agaagacggt gtcggcaccc
    85261 tgaccgattt ccgtcgcgcc gctttgcacg ttgatggttc catcctgatt catcagaagg
    85321 cgcgcgcctg ctatttctac gccgacaggc caggtgttag aggtgtagct aaaacaggcg
    85381 acgccaacgc cgcggcgtaa attgccttgc tggttctggc actctgcacg gcgtttttcc
    85441 cattcaaaga ttttccggcc tttttcaaga cactccggca accctgcgct gtaaatacgt
    85501 ttgcccgtga gcggattagc atctccttcg cgtgaggcgt tgcgtaaacg aatttcaaca
    85561 ggatcaatac ctaacgctgt cgcggcgtca tcaagcatag actcaacggc aaatacgact
    85621 tgtggcgcgc cataaccacg catcgcacca gccgagggga ggttggtata gcaggtcttt
    85681 gaactgtaag cgtaggcaca acgaggataa aggtaagcga ctttatttcc ccctgcagaa
    85741 gcgatggagt gcccgtgaga tacataagcg ccggtgttag acagaacatc cagactataa
    85801 cctttcaatg ttccgtcgcg gttcacgccc atttgcccgt caatagtaaa agcgtggcgg
    85861 gtacgggttg cgaggaaaca ctcttcacgg ctaagggaaa ctttcaccgg aatgccgcca
    85921 agcttgctgg tcaggaatgc cgccattggc tcttccagta catcctgttt attaccaaaa
    85981 ccgccaccga caaatggttt gatgactcgt acgcatgacc agggaatatc cagcgcctga
    86041 ccaaccacgc ggcgaacaat gtgcgggatc tgggtgctgg aaacgatggt aattcgcgag
    86101 tcatcctcca tccacgccag cgatgttacg ctttccatgt gacaatgttg aataacgggg
    86161 gtctgatagt gcccctgtac ctggtagtcg gcggcatcga ttgtttgttg gacattgccc
    86221 gtcgacatcg tgctttgttt cagtaaattg ccgccgttat ggattggtgc agcgtcctct
    86281 gccagcgccg cttctggcgt ggtgataacg ggtaattctt cccactcaat gctgaccaat
    86341 tgcgccgctt tttctgccgt gagttcatcg cgggccacga cgatggcaac ggcgtcacca
    86401 tgatgacgaa catggcgagt tagcagtgcg cgatcggcgg tatcgcgctt gttttcgtca
    86461 agtgtccatg catgcccagc tgtagcgaat gggatatcag gcacatcttc ccaggtaaaa
    86521 atcgccagta cgcctggtaa acttctggct tgttcatcat taatacttac ggcataacca
    86581 tgtgcgatag ggctacgtac atatttcgca taacacatgc ccgccataac ataatcgtca
    86641 gtatatcgtg cccgcccggt gaccttagca atggcatcaa cgcgcatgca tgattcaccc
    86701 gtagcggttg cttcccgcgc ttccatagaa atcccctcga tttgaacatt tggttcttat
    86761 ggcaaagatt tagtctaagc aggcaagatt tacgccagaa acggcgataa gcgttaaggg
    86821 aggaaggaag tgtgatgatg ttaaaaaaac acagatatga tacgaaacag gaccctgaat
    86881 aggggtagtg atacgtatca atatgagtgg cgataaaatc aatgtgataa attaggatta
    86941 ttacgcatca ggatgggaaa tagggtaaaa aagtcaggaa cttcgatacc tctacagtga
    87001 atattttgct gatgatactt ccctttgcca aatgaaatta acctctttct ctattacatc
    87061 gtttttccag aagcaagaga ttgggtatca agaggctggc tgctatgata tggcgtcttg
    87121 ttttttaagc gaggaaagat tttgagtgcg ggacgcctga ataaaaaatc tctgggtatc
    87181 gtgatgttgt tatcggttgg actgcttttg gcgggctgtt cgggtagcaa atcatccgat
    87241 acaggaacgt attccggctc cgtttacacc gtgaaacggg gggatacgct atatcgtatt
    87301 tcgcgcacca cgggaaccag cgtaaaagag ctggcgcgac tgaacggcat ttccccccct
    87361 tacaccattg aagttggtca gaaactaaaa ctgggtgggg cgaaaagtag cagtagtaca
    87421 cgtaaatcaa ccgccaaatc aacgaccaaa accgcatcgg ttacaccgtc atcagcggta
    87481 ccgaaatctt cctggccgcc agtagggcaa cgttgttggt tatggccaac gacagggaaa
    87541 gttatcatgc cgtattcgac agcagatggc ggcaataaag ggattgatat ctcagctcca
    87601 cggggtacac ctatttacgc cgcgggtgca ggaaaggtgg tgtatgtggg caaccagctg
    87661 cgtggctacg gtaatctcat catgattaaa cacagtgaag attacattac ggcttacgcc
    87721 cataatgaca cgatgctggt aaataatggg caaagcgtga aggctgggca aaaaatcgcc
    87781 accatgggga gcacggatgc ggcatctgtt cgcctgcatt tccagattcg ttaccgtgca
    87841 acggcaattg atccgctacg ttacttgccg cctcagggca gcaagccaaa atgctgatgg
    87901 cgaattaatc agcagtcagc agcgcggcac ttgctaagaa gagcgtaagg tttataatgc
    87961 cttacgcatc tcgaagcggg cgtagttcaa tggtagaacg agagcttccc aagctctata
    88021 cgagggttcg attcccttcg cccgctccaa tttatcttca cctcatcaat ctgccatttt
    88081 tgctgtatct gattttctat attatcttaa ttcaatcgct tcagactttt cgcaatctct
    88141 gtaacactcc cccatttttt tgtaaataaa aagcgtttac gcaatgcaat ccttttgtaa
    88201 caacccaggt aaaatacctt attttcaagt tgttaaataa gtggttatct acgcttttaa
    88261 caaacgattg atggaatatt ttatgaaagg gaaatctgca ttaactcttc tgctcgctgg
    88321 tatattttcg tgcggcacat gccaggcgac aggggcagaa gtgacgagtg aatctgtatt
    88381 taacattctt aattccacgg gggcagcaac agataaaagt tatttatcgt taaatccaga
    88441 taagtatcct aattatcgtc ttctgattca ctcagccaaa ttacaaaatg aaataaagag
    88501 ccactatacc aaagacgaaa ttcaaggatt gctaacatta acagaaaaca cacgaaagtt
    88561 aacgttgacg gagaaaccct ggggaacgtt tattcttgct tctactttcg aagatgacaa
    88621 aacagcggca gaaccccact atgatgctgt ctggctgagg gatagtttat ggggctatat
    88681 ggcattagta tcagaccagg gaaatagtgt agccgcaaaa aaggttctgc tcaccctgtg
    88741 ggattatatg tctacgcccg accagattaa gcggatgcag gacatcatta gcaacccaaa
    88801 acgactggat ggtattcccg ggcaaatgaa tgctgtgcat atttgacagc aactcccccg
    88861 tgatggcaga tgtgcaggaa gaaggcaagc cgcagctgtg gaaccataaa caaaatgatg
    88921 cgttaggtct ttatcttgat ctccttatac aagcaatcaa tactggtact attaacgctg
    88981 aagattggca aaaaggtgac cgactaaaat cggttgcgct gctgattgct tatctggata
    89041 aagccaattt ctatgttatg gaagattccg gagcgtggga ggaagatgcc cgattaaaca
    89101 catcttccgt tgcactggtg acctctggac tggaacgttt gtcaaaccta ctctcgaaga
    89161 aagactctgt ttttgtctct gatttactac gcgaagctaa agtcaatgaa ctggatgaaa
    89221 ctctttcaac aacgaggttg aaccacctca tagataaagg ttatgaaaga ataacgttac
    89281 agcttgattt agggggagaa tccccaggtt atctggaaaa agataaacat tacagagaag
    89341 ccgatgcggc tttacttaat gttatttatc ctgcaaatct ttcaaagata aacaccagaa
    89401 gaaaagagca agttctaaaa atagttaaaa aactagcagg tccgtatggt ataaaaagat
    89461 acgagaagga taattatcag tcggcaaatt tttggttcaa tgatattaaa accgataccg
    89521 atcaaaattc gcatgcaaaa agagaaaaga gcttcattcc atcaacagaa gcagaatggt
    89581 tttttgactc atggtatgca aaatcagcag caattgttta taaagaatca agaaaagaag
    89641 agtatttgaa tgactcagta caatttatga atagatcatt ggcccaaata acaggcgaga
    89701 atatgattgg tgcaaatggc aggagcgtac cagaaatggc acttcccgaa agctataact
    89761 atatccacaa gtcgggaact ttgcatgaag cgccaagccc tatcattccg ctaaattggt
    89821 cgaaagcttc aatgactctc atgttgaaag agatgtccaa ccttatcaat gatgagggaa
    89881 ttaaataatt ttcataatga ggcggtcctg gattaaaagg accgctcaaa gagaaatcga
    89941 aaggggaggt aaaagtgatc aataaattac tgttagctta cctgataggt ctggttgtga
    90001 catcgacttt aatatttatc ttctcggaag aaaaagtaac atatcggttg tttgctgcaa
    90061 taattacagg tttaacctgg ccgcttagtc tcattccttc cattataagt ctcatgattc
    90121 gtaaatccga ctaacaagaa aattatatga tcgattaaga aaaatagaat cgaacaagag
    90181 aataaaatat atgcggggag taacaaaaca ctcgcagtgt gttaatgaag aactaacaaa
    90241 tatgatttgt attgaaaata gttaccaaac aagtgttata agtaatacga ggtgtgttta
    90301 agtaaaacat atcaacagtt acaaatctgt atatggacag gatgacgtac tagtgtaagt
    90361 ggctgatagc tattcgctca taacaaaatc attattttat aaatagtaat catcactttt
    90421 taatcatgtt tttatagcaa gtttatggag aatgagaata tggaataagg agatgaaaca
    90481 tgattgaaga agggctgtta cttccaatga atgttttgct tcaaggacag aagttaaccc
    90541 tactggaccc tgaaatgtgg ttcttacggg gaaatgaaaa caaggatgta acccttgtta
    90601 ttacgattgg taataaccat caaaaattaa tggtagtgga agatatatta ctgctcattg
    90661 accgaagcca aattgaggta actgcaggga aagtaattta tcatccactt cgcattgata
    90721 tcctgagtaa attattagct tttatagatg aatcaacggg gagtgtggaa agggaacacc
    90781 atgaattgtt tgctgatgcc ttgccctttg catcagaagt tgtgctattc agtaaagttg
    90841 caagtgaagc ctggttttta gccacttatc tgagcagcga taatattaat gaaatcctgt
    90901 ttcagcatct aagaaaaaca gaatgctata aactggttcg ttatttatta tcgcaatcgc
    90961 tcatacagac ttcactctat gatcttggtg agctttacgg cgtatcgtat tcacattttc
    91021 gccgtttatg tagttatgca ttgggtggta aagttaaaac ggaattatgt ggctggagag
    91081 ttgccagagc cgtgttggaa atcattgaag gaaacagtga tatgacaact attgcgcata
    91141 aatatggtta ctcgtcctca tcacactttt cagccgaagt aaaaagccgg ttaggaaaaa
    91201 caccgagaga actatgtaaa aagttatgaa aataaaatta cgcattacta ttatattaat
    91261 ttcagtctta tgcattttta atggattatt gactcctggt gcatatgccg cagcagcgaa
    91321 tggatacgta gcaaataaag aaaatctacg tagttttttt gaaacggttt catcatatgc
    91381 gggtaagcct acaatagtta gtaagcttgc catgaagaaa cagatcagcg gaaattttga
    91441 tttaacagaa ccctatgcct tgattgaacg cctgtcggca cagatggggc ttatttggta
    91501 tgatgatggc aaagctattt atatctatga ctcatcagag atgcgtaatg cattgattaa
    91561 tctgagaaag gtatcgacga atgagtttaa taatttctta aaaaaatcgg gtctgtataa
    91621 ctctcgttat gaaattaaag gggacggcaa tggtactttc tatgtatcgg ggccaccggt
    91681 ttatgttgac ctggtcgtta atgctgcgaa attaatggag caaaacagcg atggcattga
    91741 gatcggtcga aataaagtag gaataattca tctggtcaac acattcgtta atgaccggac
    91801 ttatgagtta cgtggcgaaa agatagttat tcctggcatg gcaaaagtac tatcgacgtt
    91861 gttaaataat aacattaagc aaagcacagg ggtgaacgta ctttctgaaa tttcaagtcg
    91921 tcaacaacta aaaaacgtat cacgtatgcc tccttttcct ggggctgaag aagatgatga
    91981 cctacaagta gagaaaataa tatcgacagc aggagcgcca gagactgacg atatacaaat
    92041 tattgcatat cctgatacca acagtttact tgttaaagga acagtatctc aggttgattt
    92101 tatcgagaag ttagtagcta cgcttgatat tccaaagcga cacattgaat tatctttatg
    92161 gataatagat attgataaga ctgatctaga acagctaggt gctgactggt cgggcacgat
    92221 aaaaattgga tccagcttaa gtgcatcatt taataattca ggttcaatta gtacccttga
    92281 tgggacacag tttattgcaa caatacaggc acttgcacaa aaaagaagag ccgccgttgt
    92341 tgctcgtccc gtcgttttaa cacaagagaa tattcctgct atatttgata ataaccgcac
    92401 tttctatact aaattagtcg gcgagcgtac tgcggaattg gatgaagtaa cctatggtac
    92461 gatgattagc gtgctaccac gatttgctgc gcgaaatcaa attgaattac tcttgaatat
    92521 tgaagatgga aatgaaatca attccgacaa gaccaatgtt gatgatctgc ctcaggttgg
    92581 tagaacactt ataagtacta ttgcacgcgt gccccaaggg aaaagtcttt tgattggggg
    92641 atatacacgc gatacgaata cctatgaaag tcgcaagatc ccaatattag gcagcattcc
    92701 atttattggt aaattatttg gttatgaagg aacaaatgcg aataacatcg ttcgtgtctt
    92761 tcttattgag ccaagagaga tcgatgaacg catgatgaat aatgcgaatg aggctgcggt
    92821 tgatgccaga gcgattacac agcaaatggc aaaaaataaa gaaatcaacg atgaattact
    92881 gcagaaatgg ataaaaactt acctgaatcg tgaagtcgtc ggaggatagt gaaatggcga
    92941 ttcatgtaga gcatgtcggt gtattagaaa gagccaggga agtttctcgc ttagaggaca
    93001 ttatcacaga agataatgaa gacattgaag ctgaaatgcc taaaatgagg gatgatcccg
    93061 caggtaaaga agctcggttc ttacaagcta ctgacgagat gtcagcagcg ttaacgcagt
    93121 ttatgaagaa aaaaatttac gaagaacaac tggccaattt tctggacgga gaagaatatg
    93181 tcctggaaga ccagccaata gaaaaaaccg acaaagttat ggaggcgctt aaggctgcaa
    93241 ctacccatga ctatgaagtg tatagctttg ctaaaaaatt attcccagat gaaagcgatc
    93301 tggtagttgt gttacgtgca attttacgta aaaaacaaat atcagaaaat gtccggctaa
    93361 acgctgaagc attacttaga aaagttaatc aagagactac taaaaaattc attaatagcg
    93421 gaattaactc ggcattaaaa gcaaagttat ttggccaggc gctgtcatta aatccaaaat
    93481 tactaagggc gagttatcgg caatttttaa tggcagaaga cgatgctgta gatacttatg
    93541 tcgaatggat tggtagttat ggctatcaaa atagaatgtt ggtgacaaaa tttatcaaag
    93601 agacgctttt ctcagatata aatgcacttg atgcgagttg ttcatcattg gaatttggta
    93661 tgtttctcaa taagttgtca caactattgt ctcttcagtc ggcagaggct ttatttctga
    93721 aaactttaat gaataaccca ataattaaaa aatttatcag cgcagaagat tactggatat
    93781 tttttctgat atcgttaata aaatttcctg agactgcaga ggaactcctg aacaatgcac
    93841 tggtgaccct tcccgcagat gctaattata aagacaaaac gttactcctg aaggcgattt
    93901 acagtggatg tacaaatctt ccattttcac tttttattaa taatgaacaa ttattagaga
    93961 ttagggagtg ttgtaaacag gcaataaaag taacatttgc agcagagctc tttgatacac
    94021 aaaactgtaa caaaaaacaa aataaaaaac catggaagaa ggtgatgttc aatgtttaac
    94081 aaagttttgg tagggttaag gagtcatcca gaattaataa ttcttggatt aatggtgatg
    94141 attatcgcca tgctcataat tccattgccc acctatttaa tcgacttttt gattgggctt
    94201 aacttaacat tggcaatact tgtattttta ggatcattct atgttgatag gatcttaagc
    94261 ttttcttcat ttccatcaat cctccttatc acaacgttat ttcgtttggc gctggcgatc
    94321 agtaccagtc gacttatact tcttgaggcg gatgctggag aaattataac gagttttggt
    94381 gagttcgtta ttggagatag cctggtagtt ggttttgtta ttttctccat tgttactatt
    94441 gttcagttta tcgtgattac taaaggttcg gaacgtgtgg ctgaagttgc tgcccgcttt
    94501 tcgcttgacg gtatgccagg taaacaaatg agcattgacg ccgatctacg cgccgggatc
    94561 attgatgcag atttagcaaa agaacgtcgt agtgtattag aacgagagag ccagttgtat
    94621 ggttcctttg atggtgcgat gaaatttatc aaaggtgatg ctattgcaaa cattattatt
    94681 atctttgtta atattatcgg tgggctatca gtaggagtag gccaaaatgg aatggatttc
    94741 tcaacagcgt tgactgtcta cacaattctt actgtcggtg atggtcttgt ttctcaaatt
    94801 ccagctttat taattgctat tagcgcagga tttatcgtta cgcgcgtaaa tggcgatagt
    94861 gataatatgg ggcaaaatat aatgtcccag ttattaagta attcttttgt cattgttgtg
    94921 acatgtgtcc tcgcgctaag tattgggttg cttccaggtt ttccccttct agtgttttta
    94981 tgtttggcag taatcttagg aatttacttt tactttaaat tcaaaaagaa agggactgaa
    95041 gaaacggttg ttgagggaga tattaccgtt gggttggaac cctataatca agatgatgat
    95101 atctcattag gtatcattaa taaacttgat caggttatta ccgaaacggt acccttggtc
    95161 ttgattatga atagtgtaca ggctaaaaaa tacactgaaa tcaatcttgc tgataggatt
    95221 cgtagtcagt tttttattga gtatggtatc cgcattcccg gtattgtcat tcgtgagggg
    95281 gaaggtctta atgatgaaga tgtcatatta atgctaaatg aagtcagagc ttcacaattt
    95341 aaaatttatc atgatcttgt cttgttggtt gaatattcag atgaagtggt ttcgactttg
    95401 atcaagaaac ctgtcattgt aaatagtaat ggtgaacaat attattgggt tacgaaaagt
    95461 gatgctcaaa aattaacaaa aattggctgt tatactcgca cggccatgga tgaaatgtat
    95521 aatcatttat ctgtctgtct ggctcataat attaatgagt actttggtat acaagaaaca
    95581 aaatatatt
//
LOCUS       DAZFBR010000005        90289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFBR010000005 DAZFBR010000000
VERSION     DAZFBR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.005"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            130..1728
                     /gene="rtcR"
                     /locus_tag="ACHJZ2_000428"
     CDS             130..1728
                     /gene="rtcR"
                     /locus_tag="ACHJZ2_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO4566604.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1710..2468)
                     /locus_tag="ACHJZ2_000429"
     CDS             complement(1710..2468)
                     /locus_tag="ACHJZ2_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO4566605.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(2485..3315)
                     /gene="glpG"
                     /locus_tag="ACHJZ2_000430"
     CDS             complement(2485..3315)
                     /gene="glpG"
                     /locus_tag="ACHJZ2_000430"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO4566606.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3360..3686)
                     /gene="glpE"
                     /locus_tag="ACHJZ2_000431"
     CDS             complement(3360..3686)
                     /gene="glpE"
                     /locus_tag="ACHJZ2_000431"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO4566607.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3876..5381
                     /gene="glpD"
                     /locus_tag="ACHJZ2_000432"
     CDS             3876..5381
                     /gene="glpD"
                     /locus_tag="ACHJZ2_000432"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO4566608.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5596..6198)
                     /locus_tag="ACHJZ2_000433"
     CDS             complement(5596..6198)
                     /locus_tag="ACHJZ2_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566609.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(6198..7190)
                     /locus_tag="ACHJZ2_000434"
     CDS             complement(6198..7190)
                     /locus_tag="ACHJZ2_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4566610.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(7216..8724)
                     /locus_tag="ACHJZ2_000435"
     CDS             complement(7216..8724)
                     /locus_tag="ACHJZ2_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4566611.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(8849..11296)
                     /gene="glgP"
                     /locus_tag="ACHJZ2_000436"
     CDS             complement(8849..11296)
                     /gene="glgP"
                     /locus_tag="ACHJZ2_000436"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO4566612.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11315..12748)
                     /gene="glgA"
                     /locus_tag="ACHJZ2_000437"
     CDS             complement(11315..12748)
                     /gene="glgA"
                     /locus_tag="ACHJZ2_000437"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO4566613.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(12748..14043)
                     /gene="glgC"
                     /locus_tag="ACHJZ2_000438"
     CDS             complement(12748..14043)
                     /gene="glgC"
                     /locus_tag="ACHJZ2_000438"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO4566614.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14061..16034)
                     /gene="glgX"
                     /locus_tag="ACHJZ2_000439"
     CDS             complement(14061..16034)
                     /gene="glgX"
                     /locus_tag="ACHJZ2_000439"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO4566615.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16031..18217)
                     /gene="glgB"
                     /locus_tag="ACHJZ2_000440"
     CDS             complement(16031..18217)
                     /gene="glgB"
                     /locus_tag="ACHJZ2_000440"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO4566616.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNAQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18490..19593)
                     /gene="asd"
                     /locus_tag="ACHJZ2_000441"
     CDS             complement(18490..19593)
                     /gene="asd"
                     /locus_tag="ACHJZ2_000441"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4566617.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            19785..20378
                     /gene="yhgN"
                     /locus_tag="ACHJZ2_000442"
     CDS             19785..20378
                     /gene="yhgN"
                     /locus_tag="ACHJZ2_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO4566618.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20424..21719)
                     /locus_tag="ACHJZ2_000443"
     CDS             complement(20424..21719)
                     /locus_tag="ACHJZ2_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO4566619.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(21626..23750)
                     /locus_tag="ACHJZ2_000444"
                     /pseudo
     CDS             complement(21626..23750)
                     /locus_tag="ACHJZ2_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(23890..25230)
                     /gene="gntU"
                     /locus_tag="ACHJZ2_000445"
     CDS             complement(23890..25230)
                     /gene="gntU"
                     /locus_tag="ACHJZ2_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4566620.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(25234..25761)
                     /gene="gntK"
                     /locus_tag="ACHJZ2_000446"
     CDS             complement(25234..25761)
                     /gene="gntK"
                     /locus_tag="ACHJZ2_000446"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO4566621.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(25900..26895)
                     /gene="gntR"
                     /locus_tag="ACHJZ2_000447"
     CDS             complement(25900..26895)
                     /gene="gntR"
                     /locus_tag="ACHJZ2_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO4566622.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(27119..27814)
                     /gene="yhhW"
                     /locus_tag="ACHJZ2_000448"
     CDS             complement(27119..27814)
                     /gene="yhhW"
                     /locus_tag="ACHJZ2_000448"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO4566623.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(27937..28974)
                     /gene="yhhX"
                     /locus_tag="ACHJZ2_000449"
     CDS             complement(27937..28974)
                     /gene="yhhX"
                     /locus_tag="ACHJZ2_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4566624.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            29308..29796
                     /gene="yhhY"
                     /locus_tag="ACHJZ2_000450"
     CDS             29308..29796
                     /gene="yhhY"
                     /locus_tag="ACHJZ2_000450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4566625.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
     gene            30007..30918
                     /locus_tag="ACHJZ2_000451"
     CDS             30007..30918
                     /locus_tag="ACHJZ2_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HFO4566626.1"
                     /translation="MGTAAISHLRYDLNKYALSLRKTATLASTFFIESPLVRFEYLQE
                     IENTINDITHRFNSSYDINEKARLINELKMESETARKEYQLFRQGNYDKYITTDIFEE
                     HGLIKYVNLSFDIVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLVAHGANNFYESLS
                     PLFFNEYDSGPIRELYRVIAKKMGGDINSADYAYSIVDFSITAYGGYSGMKIVPKYNR
                     LIRPSLGNRPGTGRLFHYTSVDFKNKFSLKPTPLKIIQISSTVKKFKVTFYDENTNLR
                     IKHGLNNHMPHQLLMNISGTTTGAGIQ"
     gene            complement(31652..33397)
                     /gene="ggt"
                     /locus_tag="ACHJZ2_000452"
     CDS             complement(31652..33397)
                     /gene="ggt"
                     /locus_tag="ACHJZ2_000452"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO4566627.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
     gene            33517..33957
                     /gene="yhhA"
                     /locus_tag="ACHJZ2_000453"
     CDS             33517..33957
                     /gene="yhhA"
                     /locus_tag="ACHJZ2_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO4566628.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            complement(33944..34687)
                     /gene="ugpQ"
                     /locus_tag="ACHJZ2_000454"
     CDS             complement(33944..34687)
                     /gene="ugpQ"
                     /locus_tag="ACHJZ2_000454"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4566629.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(34684..35754)
                     /gene="ugpC"
                     /locus_tag="ACHJZ2_000455"
     CDS             complement(34684..35754)
                     /gene="ugpC"
                     /locus_tag="ACHJZ2_000455"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO4566630.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(35756..36601)
                     /gene="ugpE"
                     /locus_tag="ACHJZ2_000456"
     CDS             complement(35756..36601)
                     /gene="ugpE"
                     /locus_tag="ACHJZ2_000456"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO4566631.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(36598..37485)
                     /gene="ugpA"
                     /locus_tag="ACHJZ2_000457"
     CDS             complement(36598..37485)
                     /gene="ugpA"
                     /locus_tag="ACHJZ2_000457"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO4566632.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            complement(37583..38899)
                     /gene="ugpB"
                     /locus_tag="ACHJZ2_000458"
     CDS             complement(37583..38899)
                     /gene="ugpB"
                     /locus_tag="ACHJZ2_000458"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO4566633.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(39259..40005)
                     /locus_tag="ACHJZ2_000459"
     CDS             complement(39259..40005)
                     /locus_tag="ACHJZ2_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4566634.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            complement(40124..40837)
                     /gene="livF"
                     /locus_tag="ACHJZ2_000460"
     CDS             complement(40124..40837)
                     /gene="livF"
                     /locus_tag="ACHJZ2_000460"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO4566635.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(40839..41606)
                     /gene="livG"
                     /locus_tag="ACHJZ2_000461"
     CDS             complement(40839..41606)
                     /gene="livG"
                     /locus_tag="ACHJZ2_000461"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO4566636.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(41603..42880)
                     /gene="livM"
                     /locus_tag="ACHJZ2_000462"
     CDS             complement(41603..42880)
                     /gene="livM"
                     /locus_tag="ACHJZ2_000462"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO4566637.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(42877..43803)
                     /gene="livH"
                     /locus_tag="ACHJZ2_000463"
     CDS             complement(42877..43803)
                     /gene="livH"
                     /locus_tag="ACHJZ2_000463"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO4566638.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(43851..44960)
                     /gene="livK"
                     /locus_tag="ACHJZ2_000464"
     CDS             complement(43851..44960)
                     /gene="livK"
                     /locus_tag="ACHJZ2_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO4566639.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            45384..45767
                     /gene="panM"
                     /locus_tag="ACHJZ2_000465"
     CDS             45384..45767
                     /gene="panM"
                     /locus_tag="ACHJZ2_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO4566640.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(45764..46291)
                     /gene="ataT"
                     /locus_tag="ACHJZ2_000466"
     CDS             complement(45764..46291)
                     /gene="ataT"
                     /locus_tag="ACHJZ2_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFO4566641.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(46298..46564)
                     /gene="ataR"
                     /locus_tag="ACHJZ2_000467"
     CDS             complement(46298..46564)
                     /gene="ataR"
                     /locus_tag="ACHJZ2_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFO4566642.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            complement(46714..47817)
                     /gene="livJ"
                     /locus_tag="ACHJZ2_000468"
     CDS             complement(46714..47817)
                     /gene="livJ"
                     /locus_tag="ACHJZ2_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO4566643.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(48088..48942)
                     /gene="rpoH"
                     /locus_tag="ACHJZ2_000469"
     CDS             complement(48088..48942)
                     /gene="rpoH"
                     /locus_tag="ACHJZ2_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO4566644.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(49187..50245)
                     /gene="ftsX"
                     /locus_tag="ACHJZ2_000470"
     CDS             complement(49187..50245)
                     /gene="ftsX"
                     /locus_tag="ACHJZ2_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO4566645.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(50238..50906)
                     /gene="ftsE"
                     /locus_tag="ACHJZ2_000471"
     CDS             complement(50238..50906)
                     /gene="ftsE"
                     /locus_tag="ACHJZ2_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO4566646.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(50909..52405)
                     /gene="ftsY"
                     /locus_tag="ACHJZ2_000472"
     CDS             complement(50909..52405)
                     /gene="ftsY"
                     /locus_tag="ACHJZ2_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO4566647.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            52555..53151
                     /gene="rsmD"
                     /locus_tag="ACHJZ2_000473"
     CDS             52555..53151
                     /gene="rsmD"
                     /locus_tag="ACHJZ2_000473"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO4566648.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            53141..53410
                     /gene="yhhL"
                     /locus_tag="ACHJZ2_000474"
     CDS             53141..53410
                     /gene="yhhL"
                     /locus_tag="ACHJZ2_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO4566649.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(53413..53772)
                     /gene="yhhM"
                     /locus_tag="ACHJZ2_000475"
     CDS             complement(53413..53772)
                     /gene="yhhM"
                     /locus_tag="ACHJZ2_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO4566650.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            53913..54539
                     /gene="yhhN"
                     /locus_tag="ACHJZ2_000476"
     CDS             53913..54539
                     /gene="yhhN"
                     /locus_tag="ACHJZ2_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO4566651.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            54613..56811
                     /gene="zntA"
                     /locus_tag="ACHJZ2_000477"
     CDS             54613..56811
                     /gene="zntA"
                     /locus_tag="ACHJZ2_000477"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO4566652.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(56913..57158)
                     /gene="tusA"
                     /locus_tag="ACHJZ2_000478"
     CDS             complement(56913..57158)
                     /gene="tusA"
                     /locus_tag="ACHJZ2_000478"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO4566653.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            57379..58044
                     /gene="yhhQ"
                     /locus_tag="ACHJZ2_000479"
     CDS             57379..58044
                     /gene="yhhQ"
                     /locus_tag="ACHJZ2_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO4566654.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            58117..58674
                     /gene="dcrB"
                     /locus_tag="ACHJZ2_000480"
     CDS             58117..58674
                     /gene="dcrB"
                     /locus_tag="ACHJZ2_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO4566655.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(58678..59895)
                     /gene="yhhS"
                     /locus_tag="ACHJZ2_000481"
     CDS             complement(58678..59895)
                     /gene="yhhS"
                     /locus_tag="ACHJZ2_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4566656.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            60027..61076
                     /gene="yhhT"
                     /locus_tag="ACHJZ2_000482"
     CDS             60027..61076
                     /gene="yhhT"
                     /locus_tag="ACHJZ2_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4566657.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            61468..61845
                     /locus_tag="ACHJZ2_000483"
     CDS             61468..61845
                     /locus_tag="ACHJZ2_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4566658.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            61914..62972
                     /locus_tag="ACHJZ2_000484"
     CDS             61914..62972
                     /locus_tag="ACHJZ2_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO4566659.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYATSVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            63013..63735
                     /locus_tag="ACHJZ2_000485"
     CDS             63013..63735
                     /locus_tag="ACHJZ2_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO4566660.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            63732..64553
                     /locus_tag="ACHJZ2_000486"
     CDS             63732..64553
                     /locus_tag="ACHJZ2_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO4566661.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILHQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRATDYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            64528..64785
                     /locus_tag="ACHJZ2_000487"
     CDS             64528..64785
                     /locus_tag="ACHJZ2_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO4566662.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            64797..65048
                     /locus_tag="ACHJZ2_000488"
     CDS             64797..65048
                     /locus_tag="ACHJZ2_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4566663.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            65053..65634
                     /locus_tag="ACHJZ2_000489"
     CDS             65053..65634
                     /locus_tag="ACHJZ2_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566664.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            65631..66380
                     /locus_tag="ACHJZ2_000490"
                     /pseudo
     CDS             65631..66380
                     /locus_tag="ACHJZ2_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
     gene            66367..66627
                     /locus_tag="ACHJZ2_000491"
                     /pseudo
     CDS             66367..66627
                     /locus_tag="ACHJZ2_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            66624..67208
                     /locus_tag="ACHJZ2_000492"
     CDS             66624..67208
                     /locus_tag="ACHJZ2_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO4566665.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            67210..68379
                     /locus_tag="ACHJZ2_000493"
     CDS             67210..68379
                     /locus_tag="ACHJZ2_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO4566666.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            68376..68840
                     /locus_tag="ACHJZ2_000494"
     CDS             68376..68840
                     /locus_tag="ACHJZ2_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO4566667.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            68840..69571
                     /locus_tag="ACHJZ2_000495"
     CDS             68840..69571
                     /locus_tag="ACHJZ2_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO4566668.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            69568..70797
                     /locus_tag="ACHJZ2_000496"
     CDS             69568..70797
                     /locus_tag="ACHJZ2_000496"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO4566669.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            70799..71386
                     /gene="acpT"
                     /locus_tag="ACHJZ2_000497"
     CDS             70799..71386
                     /gene="acpT"
                     /locus_tag="ACHJZ2_000497"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO4566670.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            71497..73071
                     /gene="nikA"
                     /locus_tag="ACHJZ2_000498"
     CDS             71497..73071
                     /gene="nikA"
                     /locus_tag="ACHJZ2_000498"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4566671.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLHDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            73071..74015
                     /gene="nikB"
                     /locus_tag="ACHJZ2_000499"
     CDS             73071..74015
                     /gene="nikB"
                     /locus_tag="ACHJZ2_000499"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO4566672.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            74012..74845
                     /gene="nikC"
                     /locus_tag="ACHJZ2_000500"
     CDS             74012..74845
                     /gene="nikC"
                     /locus_tag="ACHJZ2_000500"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO4566673.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            74845..75609
                     /gene="nikD"
                     /locus_tag="ACHJZ2_000501"
     CDS             74845..75609
                     /gene="nikD"
                     /locus_tag="ACHJZ2_000501"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO4566674.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            75606..76412
                     /gene="nikE"
                     /locus_tag="ACHJZ2_000502"
     CDS             75606..76412
                     /gene="nikE"
                     /locus_tag="ACHJZ2_000502"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO4566675.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            76418..76819
                     /gene="nikR"
                     /locus_tag="ACHJZ2_000503"
     CDS             76418..76819
                     /gene="nikR"
                     /locus_tag="ACHJZ2_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO4566676.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            77018..77764
                     /locus_tag="ACHJZ2_000504"
     CDS             77018..77764
                     /locus_tag="ACHJZ2_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4566677.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            77789..78262
                     /locus_tag="ACHJZ2_000505"
     CDS             77789..78262
                     /locus_tag="ACHJZ2_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4566678.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            78259..78540
                     /locus_tag="ACHJZ2_000506"
     CDS             78259..78540
                     /locus_tag="ACHJZ2_000506"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4566679.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            78617..79975
                     /locus_tag="ACHJZ2_000507"
     CDS             78617..79975
                     /locus_tag="ACHJZ2_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO4566680.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            79968..81476
                     /locus_tag="ACHJZ2_000508"
     CDS             79968..81476
                     /locus_tag="ACHJZ2_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO4566681.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            81466..81735
                     /locus_tag="ACHJZ2_000509"
     CDS             81466..81735
                     /locus_tag="ACHJZ2_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO4566682.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            81767..82627
                     /locus_tag="ACHJZ2_000510"
     CDS             81767..82627
                     /locus_tag="ACHJZ2_000510"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFO4566683.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(82677..83036)
                     /locus_tag="ACHJZ2_000511"
     CDS             complement(82677..83036)
                     /locus_tag="ACHJZ2_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO4566684.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(83033..83308)
                     /locus_tag="ACHJZ2_000512"
     CDS             complement(83033..83308)
                     /locus_tag="ACHJZ2_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO4566685.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(83381..84505)
                     /gene="yhhJ"
                     /locus_tag="ACHJZ2_000513"
     CDS             complement(83381..84505)
                     /gene="yhhJ"
                     /locus_tag="ACHJZ2_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4566686.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(84505..87240)
                     /gene="rbbA"
                     /locus_tag="ACHJZ2_000514"
     CDS             complement(84505..87240)
                     /gene="rbbA"
                     /locus_tag="ACHJZ2_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO4566687.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(87237..88304)
                     /gene="yhiI"
                     /locus_tag="ACHJZ2_000515"
     CDS             complement(87237..88304)
                     /gene="yhiI"
                     /locus_tag="ACHJZ2_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4566688.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(88367..88480)
                     /gene="yhiY"
                     /locus_tag="ACHJZ2_000516"
     CDS             complement(88367..88480)
                     /gene="yhiY"
                     /locus_tag="ACHJZ2_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO4566689.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(88670..>90289)
                     /gene="yhiJ"
                     /locus_tag="ACHJZ2_000517"
     CDS             complement(88670..>90289)
                     /gene="yhiJ"
                     /locus_tag="ACHJZ2_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4566690.1"
                     /translation="KIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDDI
                     SSIARTMLGEVLEKVDDWQIERFQQSIQDDKISFTIQTDYSEKYSMLSGMRAHILRRN
                     NHYQFIVTINSKNYGCPLDNTDINWCGIVYLLNNMTVNDNANDVAVTESYKPIWNWKI
                     SQYNVSDIKFETIIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATG
                     HLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQVDTNVNTIISILNRRDIILESLQP
                     YLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINDNVHVLAGNHETNCN
                     GNYMQNFTRMKPLDEDTYDGIKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPITV
                     SIDEMTNALDPVELAAIINKKHHTIINSKKFKTSRAISCRSFNRYFSVSTDYRPKLEA
                     LLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHTGRMFSMHNCQINPSAG
                     PEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
BASE COUNT        21792 a        24317 c        22650 g        21530 t
ORIGIN      
        1 attaaataag cagattaatt ttttaattca ttgttttaaa aagattatat ctttacgtcc
       61 gtaaacggag atttcccgca aagccaattt accgataatg aaatatcgtc ttttataagg
      121 atatctaaga tgcgtaaaac agtggctttt ggctttgtcg gaacagtgct ggattatgcc
      181 gggcgcggca gtcagcgctg gtcgaaatgg cgtccgtcac tctgtatatg ccagcaagaa
      241 tcgttagtca tcgatcgact ggaattattg catgacgccc gctcgcgctc gctatttgaa
      301 acgcttaaac gcgatatcgc cagcgtttcg ccagaaaccg aagtggtgag cgttgagatt
      361 gaactgcata acccgtggga tttcgaagag gtttacgcct gcctgcatga tttcgcccgt
      421 ggttacgagt ttcagccaga aaaagaagac tatttaattc acatcaccac cggcacccac
      481 gtcgcgcaga tttgctggtt tctgctggct gaagcgcgtt acctgcccgc ccggttgata
      541 caatcttcac ctccgcgcaa aaaagaacag ccgcgcggcg caggtgaagt gacgattatc
      601 gatctcgatt taagccttta taacgccatc gccagccgct ttgccgagga acgccagcaa
      661 acgcttgatt ttcttaagtc cggtattgcc acgcgtaacc cccacttcaa ccgcatgatt
      721 gagcagattg aaaaagtagc gattaaatcc cgcgcaccga tcctgctaaa cggcccaacc
      781 ggtgcgggca agtcatttct ggcgcgacgt atatttgaat taaaacaggc gcggcatcag
      841 tttagcggcg cgtttgtgga agtgaactgc gcaaccctgc gcggcgatac cgccatgtcg
      901 acgctgtttg gtcatgtgaa aggcgcgttt accggggcac gggaatctcg tgaaggatta
      961 ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga ttggcgaact gggcgcagac
     1021 gaacaggcaa tgctgctgaa agccattgaa gaaaaaacct tttatccgtt tggcagcgat
     1081 cgccaggtga gcagtgattt tcagcttatc gccggaacgg tgcgtgattt gcgccagctg
     1141 gttgccgatg gcaaattccg cgaagatcta tacgcgcgga tcaatctctg gaccttcacc
     1201 ctgccggggc tacgccagcg tcaggaagat attgaaccta acctggatta tgaagtggag
     1261 cgccacgctt cactcaccgg cgacagcgtg cgttttaaca ccgaagcgcg gcgcgcctgg
     1321 ctggcctttg cgacatctcc ccaggcaaca tggcgcggta actttcgcga gctttctgcc
     1381 agcgtcacgc gaatggcaac ctttgcctct agcggacgca ttactctgga cgtggttgaa
     1441 gatgagataa accgtctgcg ctataactgg caggagagcc gcccctccgc gcttacggcg
     1501 ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc gtatgcaact ggaacacgtt
     1561 atcgcaatct gccgccaggc aaagtcgctt tccgcagccg gacgccagct ttttgacgtt
     1621 tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc tacgcaaata cctggcgcgt
     1681 tttggtctga cgtgggaagc cgtgcaggat cagcacagct ccagttgaat atggtggtcc
     1741 gtcagcacct gcatcacgct tactggcggc ggggtgtcgg tgtagacggc atctaccatg
     1801 ctgatgctac ccatattgac catcgcgtta cggccaaatt tcgagtgatc gacaaccagc
     1861 ataacgtggc gcgagttctc aataatggcg cgtttggtgc gaacttcgtg gtaatcgaac
     1921 tccagcagcg agccgtcgct atcgatgccg cttatcccca gaatgccgaa atcaaggcgg
     1981 aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc cgccatcgcg gctgcgtaat
     2041 tcgccaccgg cgagaataat acggaaatcc tcttttacca tcagcgtgtt agcaacgtta
     2101 agattgttgg tgacaatgcg caaattgctg tgattgagca gcgcgtgcgc caccgcttcc
     2161 ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg ggatttgctc cgccactttg
     2221 cgggcgatgc gctctttttc ttcggtctgg gtggctttac gatcgtgcca cggcgtgtta
     2281 accgaactgg aaggcagcgc cgcaccgcca tgatggcgca ggatcaggtt ttgctccgcc
     2341 agctcattga ggtcgcggcg aatagtctgc gggctgacgg agaagtgctc caccagctcc
     2401 tcggtactga cataaccctg ctgtttaacc agttcgataa taccgttgtg acgttgtgtt
     2461 tgtttcattt ctaaatccct ggaattattt tcgttttcgc gcattgagcg aatcaacaaa
     2521 agccatcgct aaacccacgg ctaacccggc gatgtgtgct ccgttcgcca tcgacatccc
     2581 aaacaaatca aaccatccgg cgacaatcca gatcagcgca aagataatta acccacgttg
     2641 caggtaaatg ccactttgcg gatcgcgttc gccacgtagc cagacgtagc ccatcagcgc
     2701 atacaccacg ccagaaagcc cgccaaacca cggcccgctg aatttttgct gcacatagcc
     2761 gcttaacagg gcgctgataa gcgtaatgac aattagctta ccgctaccga ggcgtttttc
     2821 caccgcaccg ccgagatacc accaccagag caggttaaag aggatatgca tcagcgagaa
     2881 gtgcattaac gcgtgggtga agtaacgcca gaactcaaat ttcagtgctg gatcgaatgg
     2941 ccaagccagc cataacatca cttcctgatc gccgagaatt tgcatggcaa taaacaccac
     3001 cacgcaggcg atcatcatca cccaggtcac cggacctgcg cgttcacgca aggcggcaaa
     3061 gaaaggataa cggcgataat gcaggccact gccggtatgg cctgcctgcc agctcgccgc
     3121 cagataacgc ggatctgccg ggttttcgag aaaacgcgcc agctccgccc gtacgcgctc
     3181 ggcctgggac tcatccgcca gccagacatc gctttggtta tgttgttgaa tcgtgaggat
     3241 aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc acgcgggggt tagcaaaaga
     3301 ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa aaggggacag tataaagcgt
     3361 tacgcgccgt acgccacctc tgcgggaaac tgacgttgcc aggcttcaaa gccgccgtca
     3421 atgctataga ccacatcgta gccctgttgc agcagatact gcgccgcgcc tttgctgcta
     3481 ttgccgtgat aacacatcac catcaccgga gtgtcaaagt cgttattacg cataaaagcg
     3541 cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat gtcccattgc gaaactctgt
     3601 ggatcgcgaa tatcgaccag caccgcctct ttttcctgca acttctggtg cgcgtcggca
     3661 acgttaatac attcgaactg atccatgcgt ctctctttct ttacaaacaa gtgggcaaat
     3721 ttaccgcaca gtttacgtcg aagcggcaga taaacgccat aatgttatac atatcactct
     3781 aaaatgtttt ttcaatgtta cctaaagcgc gattctttgc taatatgttc gataacgaac
     3841 atttatgaac tttaacgaaa gtgaatgagg gcagcatgga aaccaaagat ctgattgtga
     3901 taggtggcgg catcaatggt gctggtatcg cggcagacgc cgctggacgc ggtttatccg
     3961 tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc ttccgccagt tcaaaactca
     4021 ttcacggtgg cctgcgctac cttgagcact atgaattccg cctggtcagc gaggcgctgg
     4081 ctgaacgtga agtgctgctg aaaatggccc cgcatatcgc cttcccgatg cgttttcgcc
     4141 tgccacatcg tccgcatctg cgcccggcgt ggatgattcg cattggtctg tttatgtacg
     4201 atcatctggg taaacgcacc agcctgccgg gatcaactgg tttgcgtttt ggcgcaaatt
     4261 cagtgttaaa accggaaatt aagcgcggat tcgaatattc cgactgttgg gtagacgatg
     4321 cccgtctggt gctcgccaac gcccagatgg tggtgcgtaa aggcggcgaa gtgcttactc
     4381 gtactcgtgc tacttctgct cgtcgcgaaa acggcctgtg gattgtggaa gcggaagata
     4441 tcgataccgg caaaaaatat agctggcaag cgcgcggcct ggttaacgcc accggcccgt
     4501 gggtgaaaca gttcttcgac gacgggatgc atctgccttc gccttatggc attcgcctga
     4561 tcaaaggcag ccatattgtg gtgccgcgcg tgcataccca aaagcaggcc tacattctgc
     4621 aaaacgaaga taaacgtatt gtgttcgtga tcccgtggat ggacgagttt tccatcatcg
     4681 gcaccaccga tgtggaatac aaaggcgatc cgaaagcagt gaagattgaa gagagtgaaa
     4741 tcaattacct gctgaaagtg tataacgcgc actttaaaaa acagcttagc cgtgacgata
     4801 tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga tgagtccgac tcgccgcagg
     4861 ctattacccg tgattacacc cttgatattc atgatgaaaa tggcaaagca ccgctgctgt
     4921 cggtattcgg cggtaagctg accacctacc gaaaactggc ggaacatgcg ctggaaaaac
     4981 taacgccgta ttatcagggt attggcccgg catggacgaa agagagcgtg ctaccgggtg
     5041 gcgccatcga gggcgaccgc gacgattatg ccgctcgctt acgccgccgt tatccgttcc
     5101 tgaccgaatc gctggcacgt cattacgcgc gtacttatgg cagcaacagc gagctgctgc
     5161 tcggcaatgc gggagcggta agcgatcttg gggaagattt cggtcatgag ttctacgaaa
     5221 cggagctgaa atatctggtc gaccacgagt gggtccgccg cgccgacgat gccctgtggc
     5281 gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca atctcgtgtg agtcagtggc
     5341 tggtggaata tacgcagcag aagttgtcgc tggcgtcgta aattaacgta aggtgatcag
     5401 gtcagatttc aatctggcct gagactgatg acaaacacaa aattgcctga tgcgctacgc
     5461 ttatcaggcc tacgtggttt atgcaatatg ttgaatttgc atgattttgt aggcctgata
     5521 aggcgttcac gccgcatcag gcatgaacaa agcgccacgt tgtcaacaat ctgacctact
     5581 ttttgattca gggaattacc gcaaagccca cgccatcata cccaacgtaa caaaacttaa
     5641 gcacgttccc cactcactcc acttccgtat ctggcttcta tttttgctac gccactggca
     5701 ataatatccc aacgcaaaac caagctgtgc acttatcgga ccgacaaagt cgatccactg
     5761 ttgagaccaa aatggcgaaa aattcagtgg gagccagaca actgtcagaa agaaactgcg
     5821 catattttcc agcaaagcaa tttgcgctgg cgcgacataa aaaatgatat tccacaggag
     5881 caaccagaaa taaactggtg caaataccag ccaacaatgc agtctgcttt tcaattcagg
     5941 tggcgttctg cgcgcaaatt gccaaccaac gcccaccatt gcacagaaga aaagcgcata
     6001 ttcagccgat aaccatagtt gtaattgcag aagggaaata cgcctgtgca tgagcgggat
     6061 tgagaacagt atttctccca gaaaactcag cgtgattaca caggcagcca gcgcaaccac
     6121 cgtcggccac accaacaacg gtacaataac aaagccgaga aataacggat aaatataaag
     6181 tctgattata ttcatcatta ttgtcctgat gattcggggg atgcttcata cagcggcaca
     6241 taccccacat cctctaccaa ctgttgcccc tgctgactta taaaccagtc aacaaatttc
     6301 tgcgtttccg gcgtgggatt ttcacgcgtc accatatagg catccacaat gtatggataa
     6361 gtgccgttgc ggatattttc ctcggtaggc gcaacgccat ttatagccaa aagtttaatc
     6421 tgcttgcgca ttttgagcat attgtcattc atacgagtga cgtaatagtg gaaggtgtac
     6481 ccaatggaag atggcgtgtt ttgatagtcg gcaacttcag taattaaacc gcccataaga
     6541 tcaatcacag tggatttttt agctggcagc attggggtgt cttgcataac caaccccttc
     6601 ataatcgttt ggcttccaga atcttcaggg cgctgccaga cttttatatg ttcgtcacta
     6661 ccacctactt tattccagcg gctagttttg ccgctaaaaa tatctttaac ctggtgctca
     6721 gagagagaat ttaccgggtt attaatatca acgataaaga caaaggcttc gcgggcgaaa
     6781 gcggtatatt tcagtttaac acccgcttct tctgcacgtt ttttctgtcc ttccgacggt
     6841 tgtgcaacaa aaataaccgt cgcggaacct ttaatcagac tatcgtaggc ttcagctgtt
     6901 cgggtttgct gcaaaccata cgcctgccag gccagcgagg ttcgatcctt atcttttggc
     6961 tcgggtacta atttataata cgcggaggca tacagcgggt ataaggctgt agcgccgtac
     7021 actctcggcc atcttgacca atcatcgctg taacgtaatg caggttgccc ccgcagagac
     7081 gtcagtccat ggctctcttc ttccctgacg ctgttatagt acccttcccg gtcgacacat
     7141 cccgccagta acgccgtgcc taatagtaaa acaccggtga atttattcat ctcgctgatt
     7201 cctttctctc gagcgttatt ccattgttag atacagcggc acatatccca catcttctac
     7261 cagactctgc ccctgcggcg ttaaaaacca ttcgaccagt ttttgtgttt ctgacgttgt
     7321 attttctctc gtcaccataa atgcatcgac gatgtacgca tatttgccgt tgcgaatgtt
     7381 ttccgccgtc ggtgtaatac cgttaatcgc tagcaatctt atatttttat cagcattcat
     7441 ttgcgtcgcg taatagcgga aggtatagcc tattgcgttg tttgtattac ggtattcggc
     7501 aacgacttta atcattccct ccatcacgct tgccacttcc gtttcctgcg gcgagatcat
     7561 gcggactttt ttcatgacct gtgattgcat cactgtctgg ctgccagagt cttccgggcg
     7621 ctgccaggtc tggatctcct gatcgttacc gccaacagta cgccaattgg taattgcacc
     7681 actgaaaatg tcacgcacct gttgttcagt cagggaatta accggattat ccgcattgac
     7741 gatgaaaaca aatgcttcac gggcaaatgg cgtgtatagc aaagtgacgc ccgattcctc
     7801 agcgcgtttt ttctgcccgc cggagggttg cgccacaaaa ataatatcgg catcaccttt
     7861 aacaatccgg ttatatgcat cgggggtacg agagttctcc agatattccc aaacgtgaaa
     7921 atcctctggt attacactta atgcataaaa tgcagaagca taaattgggt acgcagccgt
     7981 ggcgccatcg attcgcggcc agttttgcct aaactgaatt tgcggtttgc cacgcagcgg
     8041 cgtcagtcga ttattgagtt tatcgggtcg ccaggccctg atatcaatat tttcgcgtac
     8101 accgacacca ggattttgct gcttatataa atgggcctga tacccggcga caataccaag
     8161 taagagaagt agaaacagga gtaagttacg gcaacgtagt ccggacgtgc cggataaagg
     8221 atatctggtc ggccaggcgt atcccagcga aaaactgata tgcgccccaa cgggaataat
     8281 gaccatccac cagtactcac cacccactaa tgagactaaa aaaacgccca accagggaag
     8341 cataaggttt gccaaccaac cagctcccat ctggcgaaat tgataatctg ccagatgtag
     8401 cgtgattatc cagccaacca gggttaatag cgcgggtaag agaaaaaagc attgccgcca
     8461 gacggctatt tctctgtttt ctggcgcgct atcaccggcc cacacgcgac cagagaaaaa
     8521 tattattatc gcccccagcg tcagcataaa gacgttgagt agaagataaa ttgttggcgt
     8581 gataaggata ttacaaactt caataatacc ggtaagcccg acacccagca tggcaacaac
     8641 cgctgccaaa aattgcgcca gtatggggat gccgaaaaaa gtcattatca gcgaggtcaa
     8701 aatccatttt ctatttggca tcattctgtc cattcttcct gaatgacgga attatactcc
     8761 ccgagaaccc tcgccccctc tggacacttt tccgaaataa aggcgaaaaa aacgggacct
     8821 tgtggccccg ttctgttttt tagcgaactt acaatctcac cggatcgata tgccagatat
     8881 gatcagcgta ctctttgata gtacggtcag aagagaagta gcccatattg gcaatgttca
     8941 gcatcgcttt tgcggtccac tcttcctgaa gctcgtagag ttcatcgact ttatcctgac
     9001 aatcgacata gctgcgataa tccgccagta cctggtagtg atcgccgaag ttgatcagcg
     9061 aatcaaccag atcgcgatag cgacccggat cttccggact gaatacaccg ctgccgattt
     9121 gcgtcagcac ctgatgcagc tcctcatctt tctcgtagta ttcacgcggt ttgtagccct
     9181 gacgacgcag ttcttccact tcttccgctg tgttaccaaa aataaagata ttgtcagcac
     9241 cgacatgatc cagcatctcg acattcgcac cgtccaacgt accgatagtc agcgcaccgt
     9301 taagcgcaaa cttcatgtta ctagtgccgg aagcttccgt ccctgccagc gaaatctgtt
     9361 cagacagatc tgccgccgga atgatcaact gcgccaggct aacgctgtag ttcgggatga
     9421 acacgacttt cagcttatcg ccaatctgcg gatcgttgtt gatcactttc gctacgtcat
     9481 tgatcaaatg gataatatgc ttcgccatgt aataggccga agccgcctta ccgccaaaaa
     9541 tattcacgcg cggtacccac ttcgcatcca ggtcggcctt gatgcggtta tagcgggtaa
     9601 tcacatgcaa cacattcatc aattgacgtt tgtattcgtg aatacgtttg atttgcacat
     9661 cgaacaacgc ctttggattc accaccacat tcagctgctg ggcgatatac tccgccagac
     9721 gctttttatt ctccagcttc gcctgatgca cagcgtgatt aaccattggg aaatcacagt
     9781 gttgttgcag ctcattaagc aggctaaggt cggtgcgcca gttacggccc aggtgttcgt
     9841 ccagcacggc tgaaagcgat gggttcgcta ccgccagcca gcgacgcggc gtcacaccgt
     9901 tggtgacgtt ggtgaaacga cccgggaaga ttttcgcaaa gtcggcaaac aacgattgca
     9961 ccatcagatt agagtgcagt tccgatacac cgttaacttt gtggctcaca acaaccgcca
    10021 gccaggccat acgcacacga cgaccgttgg attcatcaat gatcgacgcc cgtcccagca
    10081 gatcggtatc gttcggatac tgttcctgca aggttttcag gaaatagtcg ttgatttcaa
    10141 agatgatctg caggtgacgc ggcagaattt tacccagcat atcaaccggc caggtttcca
    10201 gcgcctcgct catcagcgtg tggttagtgt aggagaagac ctgacaacac acctcaaacg
    10261 cgtcgtccca gctaaattgg tgctcatcga tcagcagacg catcatctca ggaatcgaca
    10321 gtaccggatg ggtatcattg agatgaatcg cgattttatc cgccaggtta tcgtaggttt
    10381 tatgcaactg ataatggcgg cttaaaatgt cctgaatggt cgaggaaacc aggaagtatt
    10441 cctgacgcag gcgcagctca cgcccggagt aggtggagtc atccggatac agtacgcgag
    10501 atacgttctc ggagtggttt ttatcttcca ctgccgcgaa gtagtcaccc tggttgaatt
    10561 taccgaggtt aatttcgcta ctggcttgcg cactccacaa acgcagcgtg ttggtcgcgt
    10621 cggtgtcgta accagggatt atctgatcgt aagcgactcc cagaatctct tcggtttcaa
    10681 tccagcgcgt ttttttacct tcctgctgaa tgcgaccacc aaaacggact ttatagcgcg
    10741 tgttgtggcg tttgaattcc cacgggttac catattccag ccagtagtca ggcgactctt
    10801 tctggctacc gttaacgatg ttctgcttga acataccgta gtcatagcgg atgccgtaac
    10861 cgcgccccgg caaccctaac gtcgccagag aatcgaggaa gcaagccgcc agacgtccca
    10921 ggccaccgtt accgaggccc gggtcatttt cttcatcaat cagctcttcg agatttaacc
    10981 ccatcgcttc cagtgcgccc tgtacatctt cgtaaattcc tagcgacaac atggcgttgg
    11041 agagcgtacg gccaatcaaa aactccatcg acaggtagta aacctgacga gtttcttgcg
    11101 acaactgggc acggtttgaa cgtaaccagc gctccacgag acgatcgcgc acagcaaata
    11161 acgttgcgtt cagccattca tgtttattgg cgacgaccgg gtcctttcca atcgtaaaca
    11221 tcagcttgta agcgatagag tgcttaagag cttctacgct aagcgtgggc gatgaatatg
    11281 taaacggagc attcatatag gcgtttcctg aaaactattt caagcgatag taaagctcac
    11341 ggtacgactt cgccgcgacc tgccagctaa aatccattgc catagcctga cgttgcacaa
    11401 accgccacag agaaggacgg gaccacagta caaaagcacg tcgaatagcc cgtaacagcg
    11461 accaggcatt actatcttca aagacaaacc cactggcgac gccatctgca aggttctcaa
    11521 gagaacagtc agaaaccgta tcagcaagcc caccggtgcg ccgcactaac ggcagcgtac
    11581 cgtacttcaa tccataaagt tgcgttaagc cgcacggttc aaaacggctg ggcaccagaa
    11641 tgacgtccgc accgcccata atgcgatgcg aaaatgcttc gtgatagcca atctgaacgc
    11701 ccacctggcc ggggtattcc gctgccgccg caaggaaacc ttcctgcagc accggatcgc
    11761 ccgcgccgag tagcgccagc tgcccgccct gctccagaag acccggtaag gcctccagca
    11821 ccaggtcgag gcctttctgg ctggtcagac ggctcaccac tgcaaaaagc ggcactttat
    11881 cgtcaacctt aagccccatt gcgatttgta actggcgctt attttccgct ttatcttcca
    11941 acgtatcgcg ggtgtaacgc gaggccaata gtaagtccgt ctctggactc cagattttct
    12001 cgtccacgcc gttcagtacg ccggaaagac gcccttcacg atgacgctgt tgcaacaaac
    12061 cttccatacc gtaggcaaac tgcggttcgg tgatctcgcg agcgtaggtt ggactgaccg
    12121 ccgtaatgtg atcggcatag tacagaccgg ccttcaggaa agagatttgt ccgttgaatt
    12181 ccagcccatg aatattaaag aatgaccatg gcaattggat gtcattcatg tgatgtgcat
    12241 aaaacatgcc ttgataggcc aggttgtgca cagtaaacac cgacttcgcc ggacgcccgc
    12301 gcgccgccag atacgcaggc gcaaggcctg catgccagtc gtgcgcatgc accacatcag
    12361 gacgccagaa tgggtcaagc ccgctggcca tttctgcccc aacccacccc agcagcgcaa
    12421 aacgcaatac gttgtcggta taggcaaata agttggtatc gtgatacggg cttcccggac
    12481 gatcatagag atgcggcgcg tcaatcaggt atatgccaac cccgttgtaa tgaccgaaca
    12541 acagcgtgat atgcccggcg aaggtatcac gacgggatac tacctgcgca tcggtcacgc
    12601 cacggcgaat atcgggaaat gcaggcaaca gtacgcgagc gtcaacgccg tctgcgattt
    12661 gtgctgcggg taatgcccca ataacatcag ccagaccgcc ggttttaagc agcgggaaca
    12721 tctctgaaca tacatgtaaa acctgcatta tcgctcctgt ttatgcccta acttccgtag
    12781 catttcgcgc gttaccagca cgatgccttc ttctgaacga tagaaacgac gtgcatcttc
    12841 ctctgcgttt tcaccaatca ccatgccttc cggaataaca caagcacgat cgatgacgca
    12901 gcggcgcaga cggcacgagc gacctaccca tacttccggt aacaatacgg cggaatcaat
    12961 gttgcagaat gaattcacgc gaacgcgcga gaacagaacg gactgcacca ccaccgaacc
    13021 ggagatcaca caaccgccgg aaaccagtga gttaagggtc atcccgtggc taccggagcg
    13081 atcctgcacg aatttcgctg gcggtaatga ttcattgtag gtgcgaattg gccaattgcg
    13141 atcgtacata tccagttccg gcaccacaga ggccagatcg aggttcgctt tccagtaagc
    13201 ttccagcgta cccacatcgc gccagtacgg ctcggcatcc ggatcggatt gtacgcaaga
    13261 gagcgggaac gggtgcgcat aggccagacc ggcttcggtg atcttgggaa tcaaatcttt
    13321 gccaaagtcg tggctggagt tctcatcgcg atcgtcttct tccagcagtt catacagata
    13381 gtcggcgtca aagacgtaga tacccatact cgccagagat ttgctcggat cgttcggcat
    13441 tgacggcggg ttagcaggtt tttcaacgaa ttcgataatt ttatcgttct catcaaccgc
    13501 cataacgcca aatgcggagg cttcttcaat cggtactggc atacaagcaa cggtgcaacg
    13561 tgcgcctttt tcgacgtgat cgataagcat acgcgagtag tcttgcttgt agatatggtc
    13621 gcccgccagg atcaccacgt attccgcttt ataacggcga ataatatcga ggttttgggt
    13681 gaccgcatct gcggtgccgc gataccagtt ttcccctttc attctctgct gtgctggcag
    13741 cagatcgaca aactcgttca tttcttcatt gaagaatgac cagccgcgct gaatgtgctg
    13801 caccagagtg tgggactggt actgggtgat cacgcccata cgacggatcc cggagttgat
    13861 gcagttagac agcgcaaagt cgataatgcg gaacttaccg ccgaagtgta cggccggttt
    13921 tgctcgctta ttggttaaat ccttcaggcg ggtaccacgt cctcccgcca gtatcagggc
    13981 aacagatttc aatggcagct ggcgcgccaa cattaagtga tcgttcttct ctaaactaac
    14041 catgactaac tcctttttta tcatctctgg aacacacaca atccgtgtgc aggtccctgc
    14101 cagacagccg taatcactgg gttatcctct ccagcgaatg ggggaatggc gtgccactcc
    14161 ccagcaggta aaacaatctc tgttacctca agcgtggcgt taattgcgat caaaaagcga
    14221 tccgagagca gaatttgcag ctgtttcggc ccgttttgcc actcatccgt gcttaaaggt
    14281 tgagcatatc gatttagcca acggacattg ccgtcgcctt cttcccacca gcgattctcc
    14341 accaaagcgg gaatacgctt gcgcagatgg attaacgcgg cggtaaatgc ggttaaacca
    14401 ctgcttgcct gcgaccagtc caaccaggtt aattggttat cctgacagta ggcattgtta
    14461 ttgccatgct ggctgtgacc atgttcgtca ccggccagta acatcggcgt accctgggag
    14521 agcaacaacg ttgttaacag ggcgtgaatg ctgtcgcgcc gccgttcaac caggtcaagg
    14581 gtaccgccta ccccttcttt accatgattg ttactgtaat tgttgttggt cccgtcgcga
    14641 ttttcttctc cgtttgcttc attgtgttta tggttgaaac aaacgcagtc gcgaagcgta
    14701 aaaccgtcat gcgcggtgac gagattaatc gcggcactcg gcagacgacc attacgttta
    14761 aaaacatcgc tggaggcagc aaaacgcccg gcaaacgccc ccagaggcaa atcataatgt
    14821 agccagaaac gacgggcagc atcgcggaaa tgatcgttcc actcggcaaa cagcggcggg
    14881 aaatttccca cctgataacc accaggagcg atatcccacg gttcagcaat taacttcacc
    14941 tgcgagagca ccgggcagtt ctggatagcg gtaaacaacg gcgcatcctg acggaactct
    15001 ggcgtacggc ccatgactgc cgccagatca aagcggaaac catcgacgtg gcaggtttct
    15061 acccaataac gcaggcaggc gctggcataa tccaccaccg ccgggtgact caaattgagc
    15121 gtgttgccac aaccggtcca gttgtgataa tcgccgtctt ctcttatcca ataatagcta
    15181 cggttgtcga tcccacgcag cgagaataac gggccgtcga ggtccagttc cgcactatgg
    15241 ttgagcacga tatcaagaat gacttcgata cccgctttat gcagtgcttt gattgcatcg
    15301 cgaaactcat gcagcgccgt ttctggcgag caggcatacg ccggatgcag cgcaaacatc
    15361 gccaccgggt tgtaacccca gtagttactt agccccatgc gttgcagacg tggttcactg
    15421 gcaaactgcg ccactggcag cagttccagc gcggtaatgc ccaattgttt caaatagttg
    15481 atcatcaccg gatgcccgag ggctttataa gtgccacgga tctcgaccgg gatctccggg
    15541 tgcaggtacg ttaatccttt aacatgggct tcataaatga tggtgctgcc ccacggcgtg
    15601 cgcggcgggg catcatcttc ccagtcatag tgatcaacca ccactacgca tttcggcgca
    15661 atggcggcgt tgtcgcgata gtcaggttca ttatgaccgg cgtgcagcag cgggttatct
    15721 ttaaactccc cgtcaatttg ccgcgcgcaa ggatcaatca acaacttcgc cgggttaaag
    15781 cgatgcccct cggcgggttg ccaggggcca tgaacgcgat aaccataacg caaacccggg
    15841 cgcgcatccg gcagataacc gtgccaaatg tcgccactgt gccctggcaa gtcatagcga
    15901 tgttcctggc cattggcgtc aaagacacac agttctaccc gctcggcatg agcggagaaa
    15961 agtgtgaagt tgacgccctg accgtcgtaa tgcgcgccga ggggagcggg tttgccaatg
    16021 gcgagttgtg tcattctgcc tcccgaacca gccagatagt ggccagcggt ggtagcgtca
    16081 ggcttagtga atgctgacga ccgtggctgg caatctcatc gctgtgtacc gtgccgccat
    16141 tacctgcatt actgccgtga tagtgcatgg aatcggtatt gaggatctca cgccatttgc
    16201 ctggctgatt aatgccgaag cgataatcat gacgcggcac cggcgtaaag ttactggcaa
    16261 cgatgatttc gttaccctct ttatcgcgac gcacaaagat cagcaccgag cgttctttgt
    16321 catccaccac cagccattca aagccgtacg ggtcaaaatc cagttcatgc atcgctttat
    16381 ggtggcggta ggtgaggttc agatcgcgca ccagacgctg gacaccgtgg tgccagttat
    16441 cgccgccttc caacagatgc cagtcgaggc tggcgtcatg gttccactcg cggccctggg
    16501 caaattcgtt ccccatgaac agcagtttct tgcccggaaa tgcccacatc cagccatagt
    16561 aagcgcgcag gttagcgaat ttctgccatg cgtcgcccgg catgcggtcg agaatcgatt
    16621 ttttaccgtg gaccacttca tcatgcgaca acggcaggac gaagttttca gtgtagttgt
    16681 agagcatccc gaaggtcagt ttatcgtgat gatactgacg ataaatcggg tcgagcttca
    16741 tgtagtccag ggtgtcatgc atccagccga ggttccactt gtaccagaag cccagaccgc
    16801 ccatatcctg cggacgagaa acgccaggga aatcggtaga ctcctcagcc attgtcaccg
    16861 caccggaaac ctgctcacca agaatacggt tggtattacg caagaattca atcgcttcaa
    16921 gattctcgcg cccgccaaat tcgttcggga tccactcccc ctctttacgg ctgtagtcac
    16981 gataaatcat cgacgccacc gcatcgacgc gcagcgcatc aataccaaaa cgttcaatcc
    17041 agtaaagcgc gttaccgacg aggaagttac tgacttcacg gcgaccatag ttgtaaatca
    17101 gcgtgttcca gtcctgatga tagccttcgc gcggatcgct gtgttcatac aagttcgtac
    17161 catcaaattc ggcaagcgca aagtcatcag tcgggaagtg gcctggcacc cagtcgagaa
    17221 tcacgttcag accagctgcg tgtgcagcat caatgaaata acggaagtcg tcgcgagtac
    17281 caaaacggcg ggttggtgca tacaggccgg ttggctgata accccaactg ccatcgaagg
    17341 gatgctcgtt aaggggcagt agttcgaggt gggtaaagcc catccattta gcataaggca
    17401 ccagttgatc ggccagctcg cggtagctca accagaaatt gttgtcggtg tggcgacgcc
    17461 aggagcccag gtgaacttca taaatagaga ttggcgcatc aaactgattc gcttttttgc
    17521 gctcttcagt ctgtacaacc ttttccggca gcccgcaaat aagagacgcg gtttccgggc
    17581 gcatttgcgc ttcgaaggca taagggtcgg acttcagacg caagttgcca ttggcatcaa
    17641 tcatctcgta tttatagagc tgagcgttat gcgccccagg gataaacagt tcccagatgc
    17701 cgctctcttt acgcaggcgc atcgggtgac ggcgaccgtc ccagtagttg aattgcccaa
    17761 ccaccgagac ccgacgggcg tttggagccc agacagagaa acgcgtacct gtgacgccat
    17821 ccatagtatc tgcatgcgcg cctaaagttt catacgggcg caggtgagta ccttcagata
    17881 atagccaggc atccatttcc tggattagcg gaccaaaacg gtaaggatca tcaatcaggt
    17941 tttgctgccc atgccagaca acagccaact gatagcggaa aaaattctta cgtcgcggaa
    18001 tgacgccact aaagaatccc cgtgagtcga gacactccag ttttgcgagt ttgcgcccgg
    18061 ttttcggttc aatcacccac acatcggtag cgtcgggtaa aagggcacgg acttccagtc
    18121 ccgcggtggt tttatgcatt cccagtacgg aaaaaggatc cgcaaaatgg cctgcaatta
    18181 gcgcgttaat cacgtctcta tcgatacgat cggacatgct tgtcttcctg ttttattgtg
    18241 tcaccccatc caattggatt tttgacttct ggttgtgaca tttttttgac ctgaacggcg
    18301 cagcactctg tgcatcctct ctgcgtcgtc ctcacttcag gtaaggctgt gaatactcat
    18361 gtattcagcc acccttaaag aatagccaat gctctattta actcccggta aatcatgaaa
    18421 catctgcgct tactcctgta ttacgcacta acaggggcgg catcgcgccc cagatttaat
    18481 gaataaagat tacgccagtt gacgaagcat ccgacgcagc ggctccgcgg ccccccacag
    18541 cagctggtcg cccacggtaa aggctgacag gaactctggt cccatattca gcttacgcag
    18601 gcggcctacc ggcgtggtca gcgtgccggt aacggcagct ggggttagct cacgcatagt
    18661 gatttcccga tcgttcggaa cgactttcgc ccacggattg tgcgcagcca gcagttcttc
    18721 cacggtcgga atcgacacat cttttttcaa tttaatagtg aatgcctggc tgtggcagcg
    18781 caatgccccg acacgcacac ataaaccatc taccggaatt acggaagatg tgttgaggat
    18841 cttgttggtt tccgcctgcc ctttccactc ttcgcggctc tgaccgttat cgagctgttt
    18901 gtcgatccac ggaatcaggc tacccgccag cggcacgcca aagttatcca ccggcagctc
    18961 accgctacgg gttaaggttg tgactttgcg ttcgatatcg agaatagcag aggacggggt
    19021 cgcgagttca tctgctacat ggccatacag atggcccatc tgggttaata actcacgcat
    19081 atgccgcgca ccaccgccgg aagcggcctg gtaggttgca acggacaccc aatcaacaag
    19141 atcattggcg aataaaccac ccaacgacat caacatcagg cttacggtac agttaccgcc
    19201 aacaaaagtc ctgatgccat tatttaatcc gtcggtaatg acgtcctgat tgacggggtc
    19261 aagaatgatg atggcgtcat ctttcatgcg cagagacgat gctgcgtcaa tccagtaacc
    19321 ctgccatccg ctttcacgaa gctttggata gatttcgttg gtataatcgc cgccctgaca
    19381 ggtcacaatg atatcgaggg cctttagcgc ctccagatca aaggcatcct gaagtgtgcc
    19441 agtggttccg ccaaaagacg gcgcagcctg accaagctga gaagtagaaa agaagacagg
    19501 gcgaatggcg tcgaagtcgc gctcttcaac catgcgttgc atgagaacgg agccgaccat
    19561 accgcgccag ccgataaaac caacattttt cataagcgtt ttttcctgca aagatgtgtg
    19621 ctgtataaat gtgccggtct cctcttggca catctttcac catacaaaaa gcagccaaag
    19681 tcgcaagtga aattaatcaa tgatagcgaa gccatcagta atgcgactta tcctgctttg
    19741 ttagcacgca gaaagtccgc ggcaattatc agggaatttg agttatgaat gaaatcattt
    19801 ctgcagcagt tttattgatc ctgattatgg atccgctcgg aaacctacct attttcatgt
    19861 ccgtactgaa acatactgaa ccgaaaagac ggcgggcaat catggtgcga gagttgctta
    19921 ttgctctcct ggtgatgctg gtgttcctgt ttgcgggtga gaaaattctg gcatttctca
    19981 gcctgcgagc agaaaccgtc tccatttctg gcggcatcat tctgtttcta attgccatta
    20041 agatgatttt ccccagcgct tcaggaaata gcagcgggct tccggcaggt gaagagccat
    20101 ttatcgtgcc gctggcaatt ccgctggtcg ccgggccaac tattctcgcc acgctgatgt
    20161 tgttgtctca tcagtacccg aatcagatgg gacatctggt gattgctctg ctgctggcct
    20221 ggggcggcac ctttgtcatc ctgctacagt cttcgctatt tttacgtctg ctgggcgaga
    20281 aaggggtgaa cgcacttgaa cgcctgatgg gattgattct ggtgatgatg gcaacccaga
    20341 tgttcctcga cggcattcga atgtggatga aggggtaagg ttcaaacagg tgctgcacac
    20401 aaccagacgc ggcacctgtt ttattacctg aactgataat gaccttcgcc caatcggata
    20461 acccttccct cttcttctgc gcggttcagc cacacactaa tttgttcgat ggttaaacca
    20521 gtacaagacg ccagcctttc aatacttatc gattcctttg caaggatcgc cagttccgcc
    20581 acaaagagtt gataaaaatc gacaggcgta tacgcgggca ttactgcgta atttgcgtca
    20641 tcttcatgca gcgaaaacag ttctggttga gcgttacgga gtgctctgtc atacatcagc
    20701 ccaggaacgt ctggcagtct gatcgtcaca ttctcagcct gttcagcact ccatgacgcc
    20761 ccattttcta ctaatcgtga attggctgat tgcatatcct gatttggttt aacctgcaca
    20821 ggcagccact gatgttttaa tgcctccatc gccccggtta tcgttccacc ggtcattccc
    20881 gcacgtacaa ccattgcgct ttcagcaagg caataaatat atttatttcg cgccatcgca
    20941 ttgccgacgg tgaaacgtac ctctgggtaa aacggcgaaa tcaacaccag gttgcctgct
    21001 ataagccctt cacgccattt cactaacgta ctcgttttta acaagctatc agctaatacg
    21061 ccaacggcag ttcccccggc ctccagtgcc gacgccattg cacattcatc aatacctcgc
    21121 gcaccaccag agataacgca aatcccctgt tgggccagtt tcgcggccag ttgttgggta
    21181 tagcgcaaat cgtcagtcgg agcatctcgc gagccaacaa tcgccatacc ttccgcttgc
    21241 agtaatgctt tattcccgca gccaaataaa acgggaggtg catccgttcg caatcggttt
    21301 ttcaagcgaa cgggataatc agcatctccg cgggttaaga tccataaacc ggcgcgttgc
    21361 cacttatcta ccgccaacgc cagactatga ccacgcgcca gcaagccaag aatacgctcc
    21421 tgagaaatac gcggatcgct ccagcctgta agtttctctt gcggttgcgg caccagaagc
    21481 tcggcgggac tgatacgttg gtgcttcagc cataatgcaa acttccccca ttcactatta
    21541 ctaagaggtt tatacttact cgccgccgcg cgagaaaaat cgctggttag caggagagtt
    21601 gcttgtgcat tggctgaaag attcatcaat tttttaccga ggtagaagca agggcaagag
    21661 gataaaccgt cgggcaacct gcctggcgta acagtgctgt tagaactgtc agcgtccatg
    21721 cagaatccac gatatcgtca accagcaacg ccggaccagg catcaaagga ggggtaatca
    21781 caaacgcccc gtcgagattt tgacactggt ggaaacggtt ttgctgcatt ttctgcggtg
    21841 gattgtccac gactttttca atggcatcaa taaaaggtaa gccaagtttc gccgccagtc
    21901 gccgggcaaa atcaggaacc aggtcgaggt gctttcaaaa tcaggaacca ggtcgaggtg
    21961 ctttaatgaa ggaacgcagc atacccaggt tggttcagga tgcggattcc agcgttggcg
    22021 aaccatttcc gcacatgcct ccaccagttc atcactgaat cgccccgttt ttttctcctg
    22081 tgctacctgc tttccccacc cggaatatcc ccaacgagaa aggattcgcc ccgtagatcc
    22141 ttgtaaactc gcaggcaagt tccctttaaa tccgtattgc gtaaaagccc cagctgccac
    22201 ctgtttattg agatttaatg acaggtcagc gtgtttaata aataagtttg ccgcatggag
    22261 taagccgcta tcaatgtcgg gcgacaacaa cggatgctga agacaactgc tacatttacc
    22321 acaatgttcg gcacctggct catcgagtgc gcggcgcaga aattgcattt tgcactcctt
    22381 ggtcgtgata tagctttcaa cgtctgccag ctcacttttt ctctgattca tcaaatggtt
    22441 aattcgttca tggtcaggag aaaaactgac aattgttctg cgccataatt tctcggtata
    22501 caccacaggc gatggatttt cagctaccaa taatttcagt gctttttcta tttgcccata
    22561 acggagattc gtccgttgtt caatgcctcg taaggtaaga ccgtcattca cgctaagtac
    22621 gttgagtatt tcatgaattt gcgcctccgc aggaaaggcg ctttcacgga agaatttatg
    22681 aatagcacgg tcttcactgc cgcaaagcag tatgccaaca gcagaatcga taccgcgacc
    22741 ggcacgcccc acctgttagt agtaaccaac tatcgaacct ggcatctggt aatgaataac
    22801 aaaccccaaa tccggtttgt caaagcccat tcccagcgcc gttgtcgcaa ccagcacttt
    22861 aattttattt gccagcaacg cctgctccag atattccctg gcagtattag aatcttccgc
    22921 gccttcacaa gtcacgccgc tgtagtaagc aaatgcactg ataccatttt tccttaacca
    22981 ctcggcgaca agttcggcat cgcgagtagt cagggtataa acaattcccg atttggaaaa
    23041 ctgagggata accgttgcta accatgccag acgggatgag tgttcaccta agactaaggc
    23101 atcaagcgcc aaactttcgc gagccagcgt tccacgctga atcacaatgt cacccaattg
    23161 ctgacggata tcctcaacga cacggttatt cgctgtcgcg gttgtaccca gaacaggggt
    23221 attcgcaggt agttgcgcaa aatatctaat atacgtcggt aatccgggcg aaaatcgtgg
    23281 ccccaatcag agatgcagtg cgcctcgtcg accacaagca agccaatatg atcggctata
    23341 ggatataaaa ccgtttcgat aaagtcctgg tttgccaaac gttccgggga aaccagcaag
    23401 caatcgacct caccttgcag caacttatcg ctaatgcgct gccattcttc tctgtttgta
    23461 gaattcaacg tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc
    23521 agggcaagca aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta
    23581 ctggcgatga agtacacagc acttttcccc cagccagtgc gttggacaac cagcagtttt
    23641 cgccgttggt tcaccaccgc gtctatggct tcccattgcc cttccctgaa agtagcggtg
    23701 gtatttccta acatccgctg taaaagcaat tcagctgcgt gcttttccat ctttatctcc
    23761 tgcgatcttt aggcagtgat tagaacggat tatttgcagg taaatttgcg agagagatca
    23821 aaaagatgaa gagaggagaa aagagaaatg aattactgcg agcaagctcg cagtaatttg
    23881 tcgcagtaat taactcaaca gctggaacgc aatcatccca acgatggcac cgacagtgcc
    23941 gaggatggtt tccatcatgg tccaggtttt cagcgtttcg gcttcggtcg cgccggtaaa
    24001 tttaccgaac aaccagaaac cagcgtcgtt aacgtggctg acaacaatcg aaccaccggc
    24061 gatacaaatc gacagcgccg ccatttgcgc accggagtag ttcagttgtt caataaccgg
    24121 catcaccagt cccaccgccg ttaaacaggc aacggttgca gaaccctgaa tgatgcgcac
    24181 tgcagcggcc agcacgaagc aggtgatggc aatcggcagg cccatgccgg ttaacgcttc
    24241 gcccagtgcc ggacctacgc cagagtcaac cagcacctgt ttgaacacgc cgcccgcacc
    24301 aatcaccagc agaatgatcc ccgccggttg cagcgcgtga ccgcaaatct ccatcacttt
    24361 gtcttttggc atgccctgac gcattgccag gccataaatc gctaccagac aagcaaccag
    24421 aatcgcggta aacggatgac caataaactc gaaccattcg taagcggtag agccttccgg
    24481 cacaaaacgc gcggcaatgg ttttcagccc taccagcacc agcggcagca ggatcagcga
    24541 caggctgaat ccgaaggatg gcattttgcc ttcgccgaga tgcggttcgc tgatgtcgtc
    24601 aggaatatgc agctcaacgt aacggctgat gaagttaccc cacagcggcc cggcaataat
    24661 cattcccgga attgccgcac acaggccaat caggatcatc cagccaaaat cggcgttcat
    24721 ctgcgatgcc agcagcattg gcgctggccc tggcaccagg aacgccgccg cggccgccac
    24781 gcctgcgaat aatgggatta ccagcttcac caggttcgta ccggtgtggc gcgccattga
    24841 gaaagcaacg ctaatcagca gaacaatcgc cacttcaaag aacagcggta gcgcacagac
    24901 cagccccgca aggccgatgg cataatgcgc gcggctgtga ccgaaggatt taagcatttt
    24961 gacggcaatc tgatcgactg cgccggtttc atgtaagatc ttgccaaaca tagctcccag
    25021 ggcgacaacc accgccagga agccgagggt gcctcccatc cctttttcca tcgtcgctgc
    25081 gattttatcg agcggcatac cggaaaaaag gccagccccc atggacacca ccattaaagc
    25141 caggaaagcg tgcatacgcg ccttcatgac taaaaacagc agcagtaaaa cagaccctac
    25201 tgctgttaaa acaagcgtta atgtagtcac tacttatttg ccttttttaa taacctcaat
    25261 ggtgcttgcc acaacacctt ccagcggttg atcgatatcc accaccagta catcggtttc
    25321 gtccgcaccc ggctcctgca gcgtttcaaa ctgcgtcacc aacatttggg ttttaaagaa
    25381 atggcctttg cgcgctttca ggcggctttc aatcacatca aaatcgcctt tcaggtagat
    25441 gaaagagaga ttcggattac cttcacgcag caagtcacga tagtgttttt tcaatgcaga
    25501 acagacgatc agcgacactt tattagtgcg ctgcatagca aacgcggcgt cgttcagcgc
    25561 ctgcaaccac ggtttgcgat cgtcgtcatt cagcggttcg ccagacgcca ttttttcgat
    25621 attgcgccgt ggatggagga aatcgccatc aagaaacgcg gcatgaagtt gatgcgccac
    25681 ttcacttgcg accgcagatt tgccgctgcc cgatacgccc atcaagacgt aaatgtggtg
    25741 atcatggtta gtcgtgctca aagtggtgcc cccacaatac aagaattaaa aatgttacgg
    25801 gtaactgtta taggtaacat tgtccagccg gacaatatca gaagcaatat ccatccgtgc
    25861 cttaagtgta taagtgtgag ctacttcaaa tttgtgggct taaatagatc cgcccggtga
    25921 caaggtgaaa cctaaatcta acattttcgg tgtcacagat tcgccacgaa tacgcgccag
    25981 caggcgttca gcgccaatac tgcccatccg ctcacgcggc gtcagcacgc tcgcaagtcg
    26041 tggctccatc acctgaccaa tgtcatgacc gtggaaaccg gcaatcgcca tatcgtcagg
    26101 aacttttaac cccagacgct gacattcaaa cgccgcgccg accgccagat catcattagt
    26161 acagaacacg ccatccagct gcggatattc ccgccgtgcc tggcgaatca gttcaatacc
    26221 ggaagagtaa gaagaagatt gctcaaccat cacgctatat ggcaccaggc ctgcatccag
    26281 catcgcctgt tcgtatccct tctgtttgat gatagtacgt tcgtcgagac gtgcgccgag
    26341 ataggcaatg tggcgatgcc cgcgagcaat aatggctgtg gtcatctggc gtgccgcttc
    26401 aaagttatca aaaccgacgg cgatatcgag acacggcgac tggctgtcca tcaactccac
    26461 caccgggatc cccgccactt caatcatctt taaggtgcgc ggcgtgtggg tacgttcggt
    26521 gaggatcagt ccgtcgatat tccatgagag cattgattcg agacgttctt gctccatttc
    26581 cggtttataa ccgtagtggg ccagcatggt ctgataaccg tgcgcgtcgg tgacgctttc
    26641 gattccgcgt aatacttccg cgaaaacctg gttggtgaga gaaggtaaca ggacgccaat
    26701 cgcccggctg gtggcgttag agaggatatc gggcgcacga ttgggaatat agcccagttc
    26761 atcaagagcc gcggcaatct tgccgcgtag agcgacggaa acctgctccg ggttgcgtaa
    26821 aaaacggctg accgtcattt tggtcacgcc tacacggtca gccacatcct gaagtacggg
    26881 tcttttcttt ttcatcgtcc tgaaggtaca aaagagatag attctcttag tttaacacgg
    26941 acgcatcaga accttcccgg ggaaaacagt gcttgtttac aattattaga tatacactaa
    27001 atcaattgca tcgcattgtg ctaacgacgc tgcaaaaccc cggaagcgta catcagcacg
    27061 cgaccggagc gagcgaggaa gcctgcaaaa aggcaggctt caaagatgcc gttgagcttt
    27121 aaaccggcgg cagatcgaac agtaacactt cgctgtcgct atccgcatgg atggagattg
    27181 cctgctcatc ccagattgcc agaccatcgc tgatcgaggc tttcacgccg ttaatggtga
    27241 cattgccttt caccacctgg atccagacgc ggcgttcagc ggcaatctga tgcaccgact
    27301 gctcatcttt cagcaacgcc cagcggtaca gttccatatc ctgatgcact ttcaatgaac
    27361 catcacgcgc atctggcgaa agcaccagtt gtttgccctg cactgcatcg aagcgacgct
    27421 gttcataacg cggcgtaatg ccgttttctt ccggcatgat ccagatctga tacaggtgca
    27481 gacgctcggt gctgcttggg ttgtactctg agtgacgaat acccgtaccc gcactcataa
    27541 tctggaactc gcccgccgga acctgctctt tattgcccat gctgtcctgg tgctcaacag
    27601 taccttccag cacgtaggtc aaaatttcca tatctttatg cgggtgagtg ccgaagccct
    27661 gccctgcttc aatcacgtcg tcgttaatca cgcgcagcgc ggagaagccc ataaagttcg
    27721 gatcgtaata gttggcaaaa gagaaagtat gccaggagtc cagccagcca tgatttgcat
    27781 gaccacgttc atttgctttg cgtaagtaga tcatttgtat tcgccccctg aatgatttcg
    27841 atgggcttag tgtggacccg atctgcctgg gatgatagag ggtgaaaatt gacccctctg
    27901 ttcaaaaaaa ttgaactatt cgaggggcca tcaaacttac ttcgcgagag tcactgtgga
    27961 gggagaggct tgctcgaatc cacgttcaag tatttccaga ttcgtaagaa cttcagattc
    28021 cttgacgtaa tttggcgcac cgtgggtgat ggtttgatac aacgcatcat aaacgcgccc
    28081 gtaatcgccc atctccggct tcatctcttc tctgaccgtc acgccctcgt cattgacata
    28141 ctccagcaca ccgaccgaat catccgctgc gaatcccggt tcgcccggca taatattagc
    28201 cttcaggctg gtttcctgct ggtcgatacc atatttaata aacgaacctt tcttaccgtg
    28261 aacgataaat ttcggataat cgattttcac cagatggctg gttttgacga tggcttttag
    28321 atcgccataa aacagttgcg cttcgaaagt atcgtcagga ttggctttat tgcgcaggct
    28381 gcggatgtca taagcgacgt gatccgggcg accgaacaga gaaataatct ggtccatcgt
    28441 atgcacacca aggccataga acgcgccatc ctgcggcagc ccaggtttgg tttctgccac
    28501 cgggcggtaa tagtcaaaat ggctttccac ttcgacaatc tctcccagct tgccgctttc
    28561 aatcgctttt ttcgccgtca ggaagcagga atcaaagcga cgattctgat atggcgtgac
    28621 ggtcagccct ttgcttttcg ccagtgcaaa cagctctttc gcctgcgcaa gtgtcggagt
    28681 gaacggtttt tcgaccagca catttttccc ggcttccatc gcgcgcttcg cgtactcgaa
    28741 gtggctgtcc gcatgggtgc agacaacaac cagcttaaca tcgggatcat ttagcacttc
    28801 gttgagatcg ctggtgaaat ggatatgaga gtaaatggga gcttgttctt ccggctttgc
    28861 atggcggcga aaaatatgcg cgacatgcca gctatccttg cggttaagta catacggcag
    28921 atggtaacgg gtggtgcttt tgccgaagcc aataaaggcg cagttgatga ccatgatttc
    28981 gtccttttta aggtggttat ttacacctta gcgcaaagcg gacgtggttc ctactggagt
    29041 gcgcataacg aacacaagca cttcccgagg ataaattgag aacgaaagat caaaaaaaaa
    29101 gccagcaccc ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg
    29161 ttctccgcga gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc
    29221 caacactttt tgcaaaaaaa tgcatttgac tcgcattcga aagtcaatga tgttgaaagg
    29281 gacatttacc ccaaagagga caaaggaatg agtgagatag tgatacgcca tgctgaaaca
    29341 cgggattacg aggccatcag gcagattcac gcccagccgg aggtgtatta caacacacta
    29401 caggtgcctc atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc
    29461 aagcaactcg tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa
    29521 cagcgcccac gccgcagtca tgttgccgat tttggtatct gtgtcgacgc ccgctggaag
    29581 aatcgcggcg tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg
    29641 cgggtagatc gtattgagct aacggtgttc gtcgataacg cgccagcaat taaggtctat
    29701 aaaaaattcg gctttgagat tgaagggact ggcaagaagt acgcattacg taatggtgaa
    29761 tatgttgatg cgtattttat ggcgcgggtg aagtaaagaa tattctcaaa ttgcctgtta
    29821 ccaatgtgaa taattagttt tgttcatcta atttgagttt ttccttatat taccaccatc
    29881 attttgaaac aatttgtaac atcaagagac ctttgaatca ttactcgtta atatttctta
    29941 ttaaagagaa catattttcg caagcaatag ttattactgt ttgtttatgg atagagagtc
    30001 tacttaatgg gtacagcagc tataagtcat ttacgatatg atcttaacaa atatgccctt
    30061 tctttacgca aaaccgcaac acttgcttca acatttttta ttgagtcccc attagttaga
    30121 ttcgagtatc tgcaagaaat agaaaatact attaatgata ttactcacag attcaattct
    30181 tcatatgaca tcaatgaaaa agcacgactt atcaatgaat taaaaatgga gtcagaaaca
    30241 gcaagaaaag aatatcaact tttcagacaa ggaaattatg ataaatatat cactactgat
    30301 attttcgaag aacatggttt gataaaatat gtgaacttaa gtttcgatat tgttgcatct
    30361 gtaggacagg tcgtaggcgg ggttggcgcg ttgaaatttg gtaaggtagt gcattcaaac
    30421 cgaataaaag gtataggcgt cacattagtt gctcatgggg cgaataattt ttatgaaagc
    30481 ttatcacctt tattttttaa cgagtatgat tcaggtccaa tacgtgaact ctacagagta
    30541 atagcaaaaa aaatgggagg tgatattaat tctgctgatt atgcatacag catcgtggac
    30601 ttttcgatta ctgcatatgg tggatattca ggcatgaaaa tcgtacctaa gtataataga
    30661 ttgattcgtc cttcattagg aaatagacct ggtacaggac gattatttca ttacacatca
    30721 gttgatttta aaaataaatt ctcattaaaa cctacccctt taaaaatcat acaaattagt
    30781 tctactgtaa aaaaattcaa ggttacattt tatgatgaaa atacaaattt gaggataaaa
    30841 catggcctca ataatcatat gccacatcaa ttattaatga atatatcagg aactacaact
    30901 ggtgcaggaa tacaataaca gaataactta attttaagat aaaacatttt gaggcataat
    30961 tggtaagaga atttagtatc cagttttctg tgtatgataa gaatagcgaa catggcaata
    31021 ctaacgattg aagacagaaa aaaacttttc aacatattat cctgaaaaaa tgagaataca
    31081 agcaatatcc cattaactat acttaccaac ccaaacccca aagagcaata acccaccatt
    31141 gaaataatag catcaatgaa attttgttct acgagagaga cttcatgctt attagcaagt
    31201 ttcttcattt ctttgtatct tgattttaaa tagaagagtt cttgtttatt aagtacagag
    31261 agaatcacgt attttgcttt cattattccc ccttaaaaac actttacaaa atcgatcaaa
    31321 gtcatgatga atgccattat gcacataaac aacacaaagc gtgatattta aatcaataaa
    31381 ttacactcta aaatgaacta taactacagt ttatttaata gcatagttaa ttcctttcct
    31441 gaatatcagg aagtaattat cgcacgtcct attttcacgg taaatgaaag actattctgc
    31501 atactcttga aaagaaagca taacctaagc cccttaaaag cattcaggat aattatgatt
    31561 atatattacg gagcaatagt aaaagcaatc cagacactaa tttatcttgt tcaagaaaac
    31621 agccctcttc ccacgaagag ggccgctaac cttagtaccc cgccgttaaa tcatccaccg
    31681 agcgcgggtc ggatgcgccg tacaactcac cgtccggccc aaccataatg ctttgtgtac
    31741 tgcccatcgc ctctttcagc gccactttct gaccttttgc ttccagcagc ttgagcgtat
    31801 ccgggctaaa ccctttttcg acacgcagct cgtccggcaa ccactgatgg tggaaacgcg
    31861 gcgcattggt cgcttcggcg acgttcatgc cataatcgat gctattcacc accatttgaa
    31921 gcactgtagt gatgatcagg ctcccgccag ggctaccggt gaccagccag gttttaccgt
    31981 ctttcaccac aatggttggt gacatcgatg acagcgggcg tttgttcggc ccgacggcgt
    32041 tggcatcacc gcccaccagc ccgtaaacgt tcggtacgcc cggtttggca gagaaatcat
    32101 ccatttggtt attgagcaaa ataccgctct cgcccgcgac aatacccgta ccgaaggtgg
    32161 tgttcagcgt ataggtcacc gccaccgcgt taccgtcttt atccaccacc gaataatggg
    32221 tagtttgatt actctcataa ggcgcaagct tgccggggcg aatctcgctg gacggtttcg
    32281 ctttattgat atcgatttgc tcggcaatag atttggcata ggctttattg gtcagcgcct
    32341 gccacgggac tttcacaaaa tccgggtcgc caagatattc cgagcggtca gcgtaggcat
    32401 atttctccgc ttctgccatg atctgcatcg cgtcggcgct gccaaaaccg tatttcttca
    32461 tatcgaagtt ttccagaata ttgaggattt gtacgatatg gatcccgccg gaggatggcg
    32521 gtggcatgga gtaaacctga tacccgcgat aatcgccgct tatcggtgtg cgttcgaccg
    32581 ctttatacgc cgctaaatct tctttagtga tcaggccacc gtttttctgc atctcctggg
    32641 caatctgttc cgctatcgtg cctttgtaga attcgtccgg gccgttttcg gcaatcatct
    32701 ccaggctctt tgccaggttc gcctgcacca gcttgtcgcc ctttttcagc ggctcgccct
    32761 ctttccagaa gatagccttg ctgttttcgt gattcggcag cacttcgctc ccgtaggttt
    32821 tgagatcgtc agccagctcg tcgttaacga taaaaccatc gcgtgccagt ttaaacgctg
    32881 gctgcaccac tttgttcaac ggcattgtgc cgtatttatc caacgccagc gagaaaccag
    32941 cgaccgtacc gggcgtgccg gaagccagat gcgaagtgag tgattttttg ctgtccgggt
    33001 tgccctggtc atcaaggaac atatcgcggg tcgctttggc gggtgccatt tcgcggaaat
    33061 cgatagcggt ggtattgcca tttttcgagc ggatcagcat aaaaccacca ccgcccagat
    33121 tccctgcctg cggatgcgtt accgccagcg cgtagcccac agccacggcg gcatcaacgg
    33181 cattcccgcc ctccttgagg atatccaccc ccacctgagt ggcagtagca tctacagatg
    33241 ccaccatccc ctgtttcgcg cgtaccggat gaaagacatc ttcctccacg ccgtaagaaa
    33301 caggagacgg cgcaggtggc gcggctaccg tactaaaaca acttcctgtg aacagagcag
    33361 caatggccac ccggcgtaaa aacgtcggtt ttatcatcgt tattctccag agattaaggg
    33421 gcaaccccag ctaagcctgg tatataactc tgaattaatc ctgatgattg caggaaagga
    33481 gtaaacttaa agaatatctt cagaggagga tacgcgatga aacgacttct gattcttacg
    33541 gcactcctgc cgtttgtcgg ctttgcacag cccattaata ctctgaacaa ccctaaccag
    33601 ccggggtatc agatccccag tcagcagcgg atgcaaaccc agatgcaaac gcagcaaatc
    33661 cagcaaaaag ggatgctgaa tcagcaactg aaaacgcaaa ctcagttgca acaacagcat
    33721 ttagaaaacc agataaacaa taattctcag cgggtgttgc agtcacagcc gggggagcga
    33781 aatcccgccc ggcagcaaat gctgcccaac accaatggcg ggatgttaaa cagcaaccgt
    33841 aatccggata gttcgttgaa tcagcagcat atgttgccgg agaggagaaa cggcgacatg
    33901 ctgaatcagc ccggcacgcc gcagcctgat ataccgttga aaactattgg gccgtaaagt
    33961 tcggaccaat cacatcaatc gcatcggtac agatgcaatc cacgccccag cgcagcaact
    34021 ctgccgcgcg ctggggttta ttgacggtat aaaccagaat ccgtaatccg gcgtctttca
    34081 actgcatcac tcgcgcttta tcgagcaact tatgattgag atgaatagag acgcagccca
    34141 gccgcgcggt cagttctcgc cagtcgtcgc gccactcatc cagcaacaaa ccgcgcggca
    34201 gttctggtac tgcctgttgt gcggcttcta aagcatcaat ctcaaacgat gacagtagcg
    34261 gcggcgtcat accggcccac agctcgcgtg ccgccagcgc caccattttg cccgttaatg
    34321 gcccagtgcc agtggtgggt ttgatttcga tattcgccat catcccatgt tcgcgacagc
    34381 gttccgccac ctgcgaaagc aacggcagcg gctcaccttt aaacgctttg ccgtaccaac
    34441 tgcctgcatc cacacgtagt aaatcctgcc agttcagttc acccgcgacg ccccagccgt
    34501 tgctggtacg ttcgagattg tcgtcatgga gcaggaagat ttcgccatct ttcgacaact
    34561 tcgcgtcaaa ttcgatcatc ttatgaccgt attttgcccc gacgtcgatt gccgccaggg
    34621 tgttttccgg ggccagttta ccgccgccac ggtgggcgac gatgcgggga taaggccagt
    34681 tactcatact cgttgtcctg tttcaccatc aaaaagatgt agctgatttt ccggcagatg
    34741 cagccacagc gtgctgcctg ccgtcgggcg ctcctgatgc gccagccgta ccaccagctt
    34801 ctgttcgccc cagcgtccgt gcgccaggtt atctgcgccg aggatctcca gcgtgtccat
    34861 caccagcggc acgccgcctt ctgcctgcga gcttagcgcg atatgttccg ggcgaatgcc
    34921 gagagtcatt ttacgccctg catactgacg gtagccaccg tttagcggca gcgcaatgcc
    34981 gccgtccagc tcgaagtgcg tgccttcgtt attcacgcgg cctgtcagca gattcatcgc
    35041 cgggctgcca ataaaactcg ccacaaacag gctggcgggc ttttcgtaga cttcaactgg
    35101 tgtgccaatc tgttcggcaa cgccgccgtt catcaccatt actcgctggg cgagggtcat
    35161 cgcctcaacc tgatcgtgag taacgtagag tgaagtcgtt ttcaggcgac ggtgcagctg
    35221 ttgcagttca agacgcatct gtacgcgcag cttggcatcg aggttagaga gtggctcatc
    35281 aaacaggaac accgccggat cgcgcacaat cgcccggccc atcgccacac gctgacgctg
    35341 accgccggaa agctcgcgcg ggcggcgctt aagcagaccg tccagttcca gaatgcgcgc
    35401 cgcctcttta acgcgctcgg caatttgctg cttgcccatg ccgcgaattt tcagccccca
    35461 cgccatgttt tcttcaacac tcatatgcgg ataaagcgcg tagttctgga acaccatcgc
    35521 aatcccgcga tctttcggct ccatttcggt cacccgctgg tcgttgatac agatatcgcc
    35581 ttccgtcacc cgctccagcc cggcaaccat gcgcagcagc gtcgatttcc cgcagcctga
    35641 cggcccgacc atcacgataa attcgccatc cgccacatca agggtcagcg gtttaatcac
    35701 ctgggttttg ccatcccagc ttttggttac tgcctgtaat ttcagtcctg ccatcttatt
    35761 tctcactatc gaccaggccg cgcacgaagg cacgctgcat cactaaaaca atcactactg
    35821 gtgggatcag ggttaacaac atcgccgcca tcactgagtt ccattctgtg gtgccttcgc
    35881 cagtggcgat catccctttg atccctgcca cggtggtgcc gagatccacg tcggtaataa
    35941 tcaacaacgg ccacaaatac tggttccagc cgtaaataaa ggtgatcaca aacaacgccg
    36001 ccagattagt tttggacaac ggaaaaacga tgtcgcaaaa gaagcgcatt ggcgacgcgc
    36061 cgtcgatccg cgcggcttcc accagttcat ccggtagggt cataaagaac tggcggaaaa
    36121 gaaacgtcgc ggtcgccgag gccatcagcg gcagcgttaa acctgcgtag ctgtcgagca
    36181 tcttcaggtt ggcgatgact tccaccgtcg ggaagatacg tacttcaacc ggcaacatca
    36241 gggtgataaa aatcatccag aaaaagaggt tacgcagcgg aaaacgaaac cagacaatgg
    36301 cgaatgccga gagcatcgag acggtaattt tgccgagcgt aatgctgaac gccatcacga
    36361 agctgttaag caacatccgc cagaacggcg cactatttgt gcctaccccg tttacccaga
    36421 tgttgtggat gttttccagc agatgcgtgc cggggatgag cgtcatcggc gcggcgtaga
    36481 cggcctgttt atccagcgtc gccgcgacaa acgccacgta cagcgggaat aggatcaccg
    36541 cgatcccgag gatcagcatg gtatggctga atatcgtcag ccacggacgg ttctcaatca
    36601 ttggtaacgc accttgcttt caacatagcg gaactgcatc accgtcagca cgatgacgag
    36661 gaacatcaac accaccgact gtgcggcaga cgaagccaga tccagtccgg taaagccttc
    36721 gcggtagatc ttataaatca gcgtagtggt ggcctgaacc ggcccgccgg acgtggcggc
    36781 gtcgatcacc gggaaggtgt cgaagaaggc atacaccagg ttcactacca gcaggaagaa
    36841 actcaccggg gcgataagcg gcagcgcaat cttaaagaag cggcgaatcg gccctgcacc
    36901 gtcgatggct gcggcttcga tcaacgaacg gggaatggat tgcagcgcgg catagaagaa
    36961 caggaagttg tagctgattt gcttccatac tgaggcaaac accaccagaa acattgcctg
    37021 accgctgttt tgcgcgtggt tccagtcgta gccgaactca gcgagaaaat gggtgatcag
    37081 cccgcgaccg gggttaaaca ggaagatcca caatacggcg gcaacggcgg gagccacggc
    37141 atacggcagc agcatcaagg tttgatagaa acgactgccg cgcacgatgt actccaccag
    37201 cgccgcaaag aacagcgata ccagcaaacc gctgacggtg acaaaggtgc tgaattttat
    37261 cgtcgtccag aaggagtcga tatagtagct gtcatgaaac agcgcgacga agttatccag
    37321 gccgacaaac cggctggaga aaccaaacgg atcgacgctt tgcagcgagt accacaacgc
    37381 ttcgcccgca ggccagataa aaaagataac ggtgatgatg agctgcggcg cgaccagcag
    37441 ataaggcagc cagcgcgagc ggaacaccgg acgggatgat gacattacgg ttaattcctg
    37501 aactgtgccg gatgcgcttc gcttatccgg cctacacgcg atgccaggtc agataagcga
    37561 aacgcatccg acattacact gattaagact tcgtcgattt ctcaaagcgg cgcagcaact
    37621 gatttccacg ctcaacggcg gtatccagtg cctgctgtgg tgtcttctta ccggtccaca
    37681 cgctctccag ctcttcatcc acaatcacgc ggatctgcgg catgttgccc agacgcagcc
    37741 ctttggtgaa cggcaacggc ggcttattca gcatctgacg cgtcgcggta tccgcccctg
    37801 ggtttttctc ataaaagccc tgctcacggg tcaggtcata cgctgctttg gtgattggca
    37861 gataaccggt tttctgatgc cactcggcag cgttttctgg cttcgcgagg aagtcgagga
    37921 acttcgccac gccggtatac gtttctttat ctttgccctg catcacccac aggctggccc
    37981 cgccgataat ggcgttttgc ggtgcatctt tcgcatcggc gtcgtaaggc atcatgccta
    38041 cgccatagtt gaatttggcg tactcgcgaa tattagcgag agaaccggaa gaggcggtgg
    38101 tcatagcgca atcaccgtta tagaacttct cggtggattc atccttacgc ccgacgtagc
    38161 tgaaatcgcc cttcttgttc atctcttcga gcatggcgat gtgtttcacc tgctccggct
    38221 tgttgaactc cagcaccgca tccgtgccgt caaagccgtt gtttttgctg gcaaacggca
    38281 gaccgttcca ggcgctaaag ttttccagtt ggatccagcc ctgccagccg ctggcgtagc
    38341 cgcacttcat gccggaggct tttagttttg cggcatagtc cgccagatcc tgccaggttt
    38401 tcggcggttg ttccgggtct aaccccgctt tcttgaaggc gtctttgttg taatagagaa
    38461 cgggagtcga gctgttgaat ggctgggaga gtaagtggcc cgttttgctg tcggagtagt
    38521 aaccggaaac cgtcggcaca aactgcgact catcgaattg aatccccgcc tctttaaaca
    38581 cgtcataaac cggtttaatg gctttcgacg ccatcatggt ggcggtgcca acttcataaa
    38641 cctgcaaaat agccggagca ttgccggtac gaaatgcggc aatccccgcg cttaaattct
    38701 gttcgtaatt gcctttatag gtcggtacaa ttttgtaatc cgggttttcg gcgttaaaac
    38761 gttgggccag agaatccacc tctttaccca gttccccttc catagaatgc cagaacggaa
    38821 tggtcgtcac tgcctgtgca ttccccatta acgccagtcc gagcgccagt gctgaagctg
    38881 tataatgtaa cggtttcatc gtttatctct cttgttgtac cgaatgcgcg aattcacgcg
    38941 ttttatgctc gcggggtaac atgacatgct cgaattacag aaaaataact tttttgttac
    39001 atttgtaaga tagtaaggtg tcagaaagat gacaaggcgg tgacggtacg ggtgagggaa
    39061 aatgggagat ggggcacgga tatcccgaga atgcactgaa aagaggcgat taacggccag
    39121 ctaatcgcca gaactcctta ccatgaagag caaaaatggc agcaaattct gcgtccaatt
    39181 ttgctgcccg atgagagcta catttttcaa ttttaacaaa agattgccgc gacactggtc
    39241 taacggcaaa gaagattttc attgcaccac actctgcttc agccgctcat tggcggtctt
    39301 aatccattcc cggtggtgtt ttctataatg aggtcttgag cgtttagccg tatcaaccac
    39361 ggcaacatac tgctcgttgg cttcacgtaa ccgactcatt ttcgccaaca tttccgcata
    39421 gtaaatacgc gcctgcgggc cggggtaata agaaatcaat acttcaaact cgctttccgc
    39481 atcagcgtat ttttcttgtg cggctaacgt tctggcaaat aacaaatgcc cgtctgccga
    39541 ctgaaaatca gggttataac gcatcacatc ttccagcgtc tgctgacagg cggcgaactc
    39601 ctgaatagca aactgtgcct gcgccaggct tagcatcatt gccgcttcat gggcgaatat
    39661 cccgcttaac gcctgctgat aatggggaac agcttcgtga taacgcccca gacgcgccag
    39721 ttcattagcc aacgcataat ggttttgatt cgtctcggca atcgccagtt cttcggttaa
    39781 tttgcgcagg tggcgctcag ggttgagttt gtcctgcagg cgcatcagca aagtatgccc
    39841 gtgacggtca gcgcccgttt caggcagcat cactatgacg aaatacgcca cgcagccaat
    39901 caccggaaaa cagaaaatga taaaaatcca gtaccgctct tgtccggtac ggtaagcgtg
    39961 aatacagcaa agcagttcaa gcatgacaac aacggcataa tgcatgggag ggaacctttc
    40021 cgtggcaaat aagtggtctg gttttaatcc tcccggtcag ttgaaacaag tctgtatacg
    40081 gacgaataac ccggcagaac ttagctgccg ggttatcgat aatttacccg cctaaatacg
    40141 cacttctcac tgcttcgttc gccagcagcg catcaccagt atcggagagc acaacatggc
    40201 cgttttccag cacgtagccg cgatcagcga gcttcagcgc ctggttggcg ttctgctcga
    40261 cgaggaagat agtcatcccc tgctcgcgca gctgctcgat ggtgtcgaaa atttgctgga
    40321 tgataatcgg cgcaagaccg agcgatggct catcaagcaa caacaaacgc ggattgctca
    40381 tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc ggacatggtg cctgcccgct
    40441 gaacacgacg ctcatgaaga cgtggaaaca gctcatacac ccactttatg cgctcctgaa
    40501 actgatcacg ttcggcaaaa aagccgccca tcgccaggtt ctcttccacc gtcatccgcg
    40561 agaagacgcg acgcccttcc gggacaatcg ctaccgcttc gcgcatgatt ttcgccgtct
    40621 gccagtcggt aatgtcttta tcatcaaaca caattcgccc gctggtggcg cgcggatcgc
    40681 cgcataacgt gccgagcaag gtggttttcc ccgccccgtt cgcgccaatc agcgtgacaa
    40741 tctcgccctg attgatatgc aggctcacct catgcagcgc ctggattttg ccgtagtggg
    40801 cgctgacttt gtcaaaggac aacatgactt tttccatctt atgcctcacc taaataggca
    40861 cggatcacgt ccgggttatt acggatttgc tccggcgtac cgtttgccag cggcgtcccc
    40921 tgattgacca cgtaaattcg gtccgaaatt cccatcacca gcttcatatc gtgttcaatc
    40981 aacaagatag tggtgttgtg atgattgcgc agttcggcaa tcagctcatc cagctctttc
    41041 gtttctttcg ggttaagacc tgccgcaggt tcgtcgagca ttaaaatctc cggctgcgtc
    41101 accatgcagc gggcaatctc cagacggcgc tggtcaccgt aggccaggtt actcgcctga
    41161 cggttggcgt gttccagcaa accaatgcgc tcaagccagg tggcggcgcg gtcgagcgct
    41221 tcactttggg cgcgacggaa agaaggcgtt ttcaacaggc cagagaacag cccggttttc
    41281 agttgctgat gctgcgccac cagcaggttt tcaattaccg tcatttcgcg gaacagacgc
    41341 acatgctgga aggtacgcac cacgcccatg cgggcgattt gctgccccgg caaaccctcc
    41401 agatgctgat cgcgcagtaa aatagtgccg ccggtgggtt tgtagaatcc ggtcaggcag
    41461 ttaaagaccg tggtttttcc ggcaccgtta ggaccgatta acgagacgat ctcctgcggg
    41521 tacaattcaa gattgacgtt attcacagcc agcaggccgc cgaagcgcat catcaggccg
    41581 ttaacagata ataatggctg actcatgcct gctctccttt cgctgcgccg tttttcagct
    41641 tcagttgcgg gcgcgtcatg ggcagcaagc cctgcggacg ccagatcatc atcagcacca
    41701 tcaaaccacc gagcattaac atgctgtatt cgttgaaatc acgcatcaac tcgcgcgaca
    41761 ccaccagcaa aattgccgcc agaattaccg caaattgcga gcccataccg ccgagcacca
    41821 ctatcgccag cacaaacgcc gattcggcaa aggtgaagga ttccgggctg acaaagccct
    41881 gacgcgcagc aaacagcgtt ccggcaaaac cggcaaacgc ggcgctgatg gtaaaggcag
    41941 tcagcttgat acgacgcggg cttaagccca gcgaacggca ggcgatttca tcttcacgca
    42001 acgcttccca ggcgcgaccc agcggcatcc gcagcaggcg gttaatgaca aacaggctta
    42061 gcaccaccag cagcaacgcc accaggtaga ggaaaatgac acgatcggag ggatcgtatt
    42121 tcaggccaaa gaaattactg aacgtgtccc agccgccttc acgagcggta cggctgaact
    42181 caaggccgaa aaaagtcggt ttcgggatct gactgatacc gttcggaccg ccggtaattt
    42241 cggtgttatt gagtagcaat atgcgcacaa tttcgccgaa accgagggta acgatcgcca
    42301 gatagtcacc gcgcaagcga agcaccggga agccgagcag gaagcccgcc gccgccgcca
    42361 tcaacccggc tatcggcaga caggtccaga agcccaagcc gtaatagtga ttgagcagcg
    42421 caaaggtata agcgccgatg gcgtaaaagc cgccgtaccc cagcaccagc agaccagaaa
    42481 gccctaccac cacgttcagc ccgagtccga ggataatgta gatcatggtc agggtggcga
    42541 tatccaccgt cccgcgtgag accataaacg gccacgccac cgcaagcacc agcagcgcca
    42601 cgagaaacag cttttgcttc accgtggagc catcaatggc gggcagaata aacttcggtc
    42661 cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa aagctggaag aaaaagacca
    42721 ccgccgtgcc gataaacacc cactgccaac ggatgtctga agccgtgtcg accaccagtt
    42781 tggtgccatc cagctccagt tgtacgccca taaagacgcc cgccaggaca aaaaacatcg
    42841 cggcagagag caacgccatt gcaatatgca tcggtttcat actttctcta cctccgggcg
    42901 acccagaata ccggtcggca tcaccagcag caccagaatc agcagggcga atgagaccac
    42961 atctttatat tccgtactca ggtaggcaga agagagcgcc tccgcaatcc ccagaatcag
    43021 cccgccaatc atcgctcccg gaatgctgcc aatcccaccg agcaccgcag cggtaaaggc
    43081 tttcatcccg gccataaagc cgatataggg gttaatgacg ccgtagaact gaccgagcag
    43141 tacgcccgcc accgccgcca tcgccgcgcc aatcacaaag gtcaaagcaa tcacccggtc
    43201 ggtgttaatg ccaagcaggc tcgccatttt cagatcttcg gcgcaggcac ggcaggcgcg
    43261 ccccatacgg gaataacgaa tgaaaatcgt cagcgccagc atggcgagga aggtgacaat
    43321 ccagatcacc gcctgcatgg tggtaataga ggcagagaag ttttcgctat gacccactac
    43381 ccactgacca ttaaacaagc tgggcagcgc cacgtcgcgc gaaccttcag tcaggctgac
    43441 gtagttttgc aggaagatgg acataccgat tgccgagatg agtgcaatca ggcgcttaga
    43501 gttacgcacc gggcggtaag ccacccgttc gatactccag ccgtaggcgc tggcaatgac
    43561 gattgcgccg acgaatcccg ccgctaccag cagccagccg gtatcaatgc ccatcatcat
    43621 cagtgcggcg atgatcataa atgagacgta gctgccgatc atgtaaacct cgccgtgggc
    43681 gaagttgatc atgccgataa tgccgtaaac catggtgtag ccgatggcaa tcagcgcgta
    43741 agtactgccc agcgtgacgc cgttaaacat ctgctgcaag aaatacaaaa actgctcaga
    43801 cataaggtaa cctttctaaa cccgcccgca tttcacgggc ggtgggattt tcacttggct
    43861 gccgtggatg aaccgtcggc gtgccactgg aagacaccaa aatcaaatcc cttaagatcg
    43921 cctttttcat cccagttcag cggcccaatc acggtgtttg caccgttagc ttttaaatct
    43981 ttcaccagcg ccagcggctc atcgctgccg gtacgttcaa gggcagttgc cagagattgc
    44041 accgccgcgt aggtgatcca gacataaggc ccggacggat ctttcttgtc tgctttcagc
    44101 gcatcaacga tgccctggtt tgccggatcc tggtcatagc gttttggcat agtgaccaac
    44161 atgccttcgg cggcatcacc ggcaatgttc gacaacgacg cattacccac accttccggc
    44221 cccataaact gggttttcag gccaacggaa cgggcctggc gcagcatctg ccccatttcc
    44281 gggtagtaac cgccgtagta aacgaagtcg atgttttctt ttttcaggcg ggcgatcagc
    44341 gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga agacgacgtt ggcgttagcc
    44401 gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc catactgttg tttgtcgtga
    44461 atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt attttgccgc cgttggcccc
    44521 tgggaagagt ccagcccggc agtacgcata atgtgttgat aaccgcgttg ggtcagctcc
    44581 gggttggtcg ctcccggcga gatcataaga ataccttcgt cttcatagat atctgacgca
    44641 ggctgggtag aagaagaaca cagatgacca ataacgtatt taatgccgtc attaacgatt
    44701 ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt cgtcatattc tacgccaacc
    44761 agcttatcgc ctttaattcc ccctttggca ttaatgtctt taatcgcctg acgcgcgccg
    44821 ttaaattcca tatcgcccca ctgggcaatc gggccggaca tcgcgccgac aacggcaact
    44881 ttaatatcgt cggctaaagc ggtgtgtgaa attgccagtg caatcatccc tgcgatgata
    44941 gttttcgcat tccgtttcat agtcaaaaat ccccattcgt gatgttgtgt tgctttgttt
    45001 ttatgtgtta acaaatcaga ctgttctttt tttatactgc actgtttttg cctgtctgat
    45061 taagggttta gcgcagtatt ttgtgataat agcgattaaa gtccctattt ttcagtcgat
    45121 taagaacaga taatattctg aatttattga tagataaaca gaaaaaagtg cctttgtcag
    45181 cataaaataa cggcacaaag ggcggaataa ttcactacca ttcaggggat tatgctgggt
    45241 atttttcatt ctctaatgtt ttaactttgt aattattgct gttaagaaat taatcacctg
    45301 ccaaaagaaa taaaaaagag aaagcctccg attaaattat ttcgctacac tggttccact
    45361 tttgtgattt acacgggtta cccatgaagc tgaccatcat tcgattagaa aaatttagcg
    45421 accaggaccg gattgacctg caaaagatct ggccggagta ttccccttcc tctttacagg
    45481 ttgacgataa ccaccgcatc tacgccgcgc gttttaacga gcgcctgctt gctgccgtgc
    45541 gggtaacctt aagcggcacc gagggggcac tggattccct gcgcgtgcgg gaagtcactc
    45601 gacgtcgcgg tgtggggcaa tatctgctgg aagaggtttt gcgtaacaat cctggcgttt
    45661 catgctggtg gatggcggat gcaggcgtgg aagatcgcgg tgtgatgacg gcgtttatgc
    45721 aggcactggg atttacggca cagcagggcg gctgggagaa gcgttaatcg ctctgtgtaa
    45781 aaagcagttc aatggatttg gttgggaaaa ataacgcatt ttcgttttcc ccgactaaag
    45841 ggataaagcc cagcgattta taaaacgtat gggctttttc attcagcgcc tcgacaaaaa
    45901 gaccgtgaat acctaccgct aaagaggctg accagacgac tttcatggca tgagcaacca
    45961 gtgttgctcc ccatccctgc ccctgtaatg aacgatcaat tgccagacgc ccaagagtaa
    46021 cgctgggaat atttttgtag ggaatttttt tctgtttcga tttcgagggc aatgcggctc
    46081 gttcaaaaca actgccgcat aatgtgtaat atcccagcac ctgacgttct ggagtgttgc
    46141 gacaaaggat atacgctcgc agaattttgt tgcgatgttg acgaacgaga tgtgtcgtca
    46201 gaaagagatt taacgcttcc tcgccgcagt cgaatcgctg tagatcataa tctgcatcat
    46261 cggtcagaat ctctatcgtc agatcatcca taaattatta cattccctga agacgttttg
    46321 ccgcacgttt tagcttttca cctggtgacg gtggattact cagcgcatcc atcacccgcg
    46381 tccaggattc ctcattgagg atcacccgcc gatgctgttc aatcacttcc gcagcccgct
    46441 gcgaagcgct gttcaacata aattggctga cggactgatt agatatcgct gccgcttcct
    46501 caatcatact tttgtcatcg tcagttaaac gcagatcgat acgctgcttt ttaacagcag
    46561 acatgtgaac tctcctgctc cgtataatat gtacggtatg tcaccgtaca ttaagcatat
    46621 cctggattgt ttaatctttc tacaaccatt tcgacaaaca caaaaaaacc cgatggcagt
    46681 tcagcgcatc gggcattttc atttatctat ctgttacttc gcatcggttg ccgtgccgtt
    46741 ggcgtgccag tcaaacacac cgaactcaaa gcctttcaga tcgcctttct catcccaggt
    46801 cagcggtccc attacggtat ccacggagtt cgctttcagg tatttggcga tttcagccgg
    46861 atcgtcagac tgattcaggc ccgcctgcaa agattgcagc gcggcgtagg tggtccaaac
    46921 gaatgcgccg cttgggtcct gttttttcgc tttgatcgca tcaacaatgg gtttgttcgc
    46981 cggaacctga tcgtagttct tcggcttggt caccagcagc ccttccgctg attcgcccgc
    47041 aatgttagac agcgagacgt tagccacgcc ttccggcccc ataaactgag ttttcaaccc
    47101 tgccgcgcgt gcctgacgca ggatttgccc catttccggg tgataaccgc cgtagtaaac
    47161 gaagtcgata ttctcttttt tcagacgcgc caccagcgtt gagaaatctt tttccccggc
    47221 ggtgatacca tcaaagaaca ccacgttagc attgcctttc ttcaggccgt cctgcaccgc
    47281 tcgtgccaga ccttcgccgt attgctgttt gtcgtgaacg atagcaatac gctgcggttt
    47341 cactttctca agaatatatt tcgccgccgt cggcccctgg tcggagtcca ggccggtggt
    47401 gcgcaggatc agctgatagc cacgggcggt cagctccggc gcggttgccg ctggggtgat
    47461 cattaaaatg ccttcgtctt cgtagatgtc agacgcaggc tgcgttgatg aagaacagag
    47521 gtgaccaatc acgtatttaa tgccgtcgtt aacgactttg ttcgccaccg caaccgcctg
    47581 tttcgggtca caggcatcgt catattttac gatttgcagt ttgttgcctt taatgccgcc
    47641 tttagcgttg atatccgcaa ccgcctgctc tgcgccggta aactcctggt caccgtactg
    47701 cgcaaccgga ccggacattg cgcccacgac cgcgacttta atatcttctg ccagagccat
    47761 attgctgaat gccagcgcga tacatcctgc cagtaacgct ttacccttta tgttcatcct
    47821 gagaatcccc attcttctgg ttattacgtg tgttgtgatg ttgttgttca gcactttatt
    47881 tcgttttatg catgactacc cgtgctttag cagcatactc tgctaaaaca tacccgattt
    47941 ttatgatatt ggaatagcta ttttgacagt ttattaacaa tctgcgtggg gattggcgtt
    48001 ttgccggagg ggaaattgat tattgtagag gcccaaaaaa caaaaacccc ggactcgcat
    48061 ccagggttct ctgcttaata gcggaaatta cgcttctata gcagcgcgca gttttttcat
    48121 cgcgttcttt tccagctggc gcacacgctc agcggaaaca ccgtaacggt cagccagttc
    48181 ctgcaacgtg gacttgttgt cttcgtccag ccagcgcgca cggatgatgt cctggctgcg
    48241 ttcgtccaga ccctgcatcg cgtcggtcag acggtttgct gcctgctctt cccagttatc
    48301 atcttcaatg ccgtcggcaa agttagatga tttatcctgc agatagagca ccggtgccat
    48361 cggttggctg tcggaatcgt cgtcggaaga caggtcaaag gtcatgtcct gtgccgccat
    48421 acgtgattcc atctcacgta cgtctttgct ggttacgccc agttcacggg ccaccatttc
    48481 gacttcatcc tggttaaacc agcccagacg ctgcttggtt ttacgcaggt tgaagaacag
    48541 tttgcgctgc gctttggtgg tcgcaacttt gacgatacgc cagttacgca gaacgtattc
    48601 gtggatctct gctttgatcc agtggacggc gaaggagacc aggcgcacac ccacttccgg
    48661 gttaaaacgg cgcactgctt tcatcaggcc gatgttacct tcctgaatca aatccgcctg
    48721 tggcaggcca tagcccgcat aattacgagc aatatgaaca acaaaccgca ggtgagacag
    48781 gatcagcgtt ttagctgctt ccagatcgcc atggtaatgc agcttttcag ccagcgcccg
    48841 ctcctcgtca gccgacaaca tcggccacgc gttagctgcc cggatgtagg aatccaggtt
    48901 gccaactggg gctaaagcta aactttgcat tttgtcagtc attcaaatcc tctcaatcga
    48961 tatcttctgg cgcttcagtg gtagcaacaa ctgtgccaga gcttaagagc aacgaggtta
    49021 tcattcactg ttttatcaga ccgtgatttt atccacaagt tcaatgcagg cttgtgaata
    49081 aattacgcac aaaatgtgac atagagatga aataccggga agagacaacg gggtctcttt
    49141 ccctgctacg gaacccattg cagggaaaga gtataacacg cttttattat tcaggcgtaa
    49201 agtggcgtaa atgttgtacc gtggcaagcc acgctgccac ccagccaatc atcgagcata
    49261 ccagcagcaa cagcaggcat tcatcgaacg ataaaccatt gatatcaaac ttcgttccga
    49321 aaacctgtgc cacttccgca accgccgatg acaatcgcag taccagaatt tctgataaaa
    49381 ttaatgacaa caatgcgcca gaaaacccca gcaatgcgcc accgtacagg aacgggcgca
    49441 ggatgaaacc atccgttgca ccaatcagtt tctgcacgtt aatggagtca cgacgagcaa
    49501 agatactcag gcgtacactg ttaccgatga cgaggaacac tgccgccacc atcaacacgc
    49561 cgatcatcgc cgaaacgcgc ccgaccagcc cggtcaacgc cgccagacgg gcaaaccagc
    49621 tgtcatccat ccgcacttcg tcaatgccgt taatctgcgt gatacgatca cgcagcgtat
    49681 tcagagactc cgtcccctga aagtcgagct tcgggatcac caccgccact gccggaagcg
    49741 ggttttcttc cagcatatcc agcgcgccac caaaaccaga ccagttacgg aactcgccca
    49801 gtgcgtcttc acgagaaaga tagttcactt tctctacgcc ttgctcggcc tgcaactgtg
    49861 ccaccacgcc cgcagcggcg tcatcgtcca gcgttttttg cagataaaca gtgatttgcg
    49921 gtgacggata atactgcgtc gccgcctggt taacgttttt gtacaccata taacagacgc
    49981 tgggcagcgt cagggagatg gcgataacca tcaccgttaa aaatgtggcg aacggtttgc
    50041 ttttcagatc ctgcaatgcg ccgtggaagg catagcgcac ctgttcgttg aaaacgttgg
    50101 ttttgcgatt taccggtttt ggcgaggatt tcgcgcgttt cggtgcgtta cgaccgccgt
    50161 cgcctgagcc gccgaccgat ttacggaagc gatcaagacg cccgccaaac tgccgaatat
    50221 gattgattgc atcgcgctta ttcatggccc acgcctccat gcaagtgacc atcgctcagg
    50281 gtgagcatgc gataggaacg ccgtgaaatc aggttgatgt cgtgcgttgc catcaatacg
    50341 gttaccccta cgcggttaaa ctcttcaaac agacgtagaa tcccttccga caacgcgtcg
    50401 tccaggttac cagtcggttc gtccgccagc agtaccgcgg gcttgttcac caccgcgcgg
    50461 gcaatgccaa cacgctgttg ttcaccgcca gaaagctgaa tagggaagtt cttcgctttg
    50521 tccagtagcc cgactttatc cagtgccgcc gacacccggc ggcgaatatc gtcaccgctg
    50581 gcaccggcga taatcagcgg gatcgccacg ttatcgtaga cagtacggtc catcagtaga
    50641 tggtgatcct ggaaaatcat gccaatctgg cggcgcagaa acggaacttc acggtttttc
    50701 agacgcgtga tgtcatggcc gctaaaccag attttcccgg cgctgggccg ctcaatccca
    50761 cagatcagct tcaggagggt acttttccct gcgccggaat gaccggtcag aaacgccatc
    50821 tcacccggct gcatatggaa cgtaacgccc tgcagcgcct gtctcccacc gagataagcc
    50881 ttgctgacat gttcaaagcg aatcattgtt aatcctctcg ggcaaaaagt gcctctataa
    50941 agtcgtccgc cttaaacgga cgcaaatcct caatacgttc gccgacacca atgtagcgga
    51001 tagggatacc aaactggtca gccaccgaga aaattacccc gcctttcgcc gtgccgtcca
    51061 gtttcgttag cgtgatgccg gttaagccaa cggcttcatg gaacagtttg gcctggctta
    51121 ccgcgttctg cccggtgctg gcatcgatag tcagcataac ttcatgcggc gcttcaacgt
    51181 cgagtttctt catcacgcgg acgattttct tcaactcttc catcaggtgc gatttgttct
    51241 gcaggcgtcc ggcggtatcg gcaatcagga cgtcgatatt acgcgcttta gccgcctgaa
    51301 tggcgtcgaa gataacagag gcggaatccg ccccggtatg ctgggcaatc accggaatat
    51361 tgttgcgctg cccccagacc tgaagctgtt caaccgctgc tgcacggaaa gtatcgcccg
    51421 ccgccagcat caccgattta ccctgctgct caaactgacg tgccagctta ccaatcgtcg
    51481 tggttttacc cacaccgttg acgcccacca tcaggatcac gaacggcgtt ttaccttcaa
    51541 cattcagcgg ctcatcgact ttcgccagaa tctcacccat ctcttctttc agcaggccat
    51601 agagcgcctc ggcatcacga agctgcttgc gggatgcgcc ttccgtcaga ttggtgataa
    51661 ttttacgtgt ggtttccacg cccacatcgg cgatcaaaag ctgctcttcc agctcctcaa
    51721 acagatcatc gtcgattttt ttaccgcgga acaggctgat aaatccggaa ccgagatttt
    51781 ctttggtttt taacaggctg cgtttcaggc gcgcgaaaaa accttctttg gtcggttttt
    51841 cctgctcctg agcgatttct tccaccggct gctcttcttc tgccggagga accaccatca
    51901 ccgcctcttc tgctgcttcg gcagccagcg cctgtgcttc cagctcttcg tcggtgattt
    51961 cttctttagc cgcttcttct tccgccgctt cgacaatctc tacggtttcc gcttcagcct
    52021 gccactcttc tggcgaaacc tcttcggcgt tgacgtcttc cggcaacggc agctcttcac
    52081 gttcgatagc cactggctcc ggcgtttctt ctacgaccgg ttccggttgt gcaacgactt
    52141 ccgcttcagg ctgcgctttt tcattttcag caacctgttc agtgacttcc acaacgtcgg
    52201 cagcaaaagt ttccgcctcg gcctcagtat gcgcctgcgg ctgctcttcg acggcttgtt
    52261 cagaagcctt cacaggctct tgcgcctgaa cgatttcttc tacaaccgtt tgttcattct
    52321 gaacttctgt ctctttttcc ggggtctgct ctttttgacc aaagcccagc caggaaaaaa
    52381 agccacgttt tttttctttc gccatttgcg actacactcc tcgctgttgc ttcatggcac
    52441 agcgttaacg ctatgtacat agcagctaaa aaaatgatga aatagtctat cacttaactt
    52501 aattcacatc accgcctgca agtatgtgtt atctggcgga ttgagcaatt tatcatgaaa
    52561 aaaccgaatc attccggcag cggccaaatc cgcattattg gcgggcagtg gcgaggccgt
    52621 aaactcccgg ttcctgatag cccaggtctg cgccccacca ccgaccgcgt acgcgaaacg
    52681 ttgtttaact ggctggctcc ggtcattgtt gacgcccaat gtctggattg cttcgccggg
    52741 agcggcgcgc tggggctgga agcgttatcg cgctacgctg cgggggccac gttaattgag
    52801 atggatcgtg cagtttctca gcagttaatt aagaatctgg cgacactaaa agcaggcaat
    52861 gcacgcgtgg tgaacagcaa cgcaatgtca ttcctggcgc aaaaaggcac accgcataat
    52921 atcgtgtttg tcgatccacc gttccgccgt ggtttgttag aagagacgat aaatttactg
    52981 gaagataacg gctggctggc tgacgaaacc ctgatttatg tcgaaagcga agtcgaaaac
    53041 ggtctgccca ccgttccggc aaactggtcg ttacatcggg aaaaagtggc gggtcaggtg
    53101 gcttatcggc tgtatcaacg cgaagcacaa ggagaaagtg atgctgatta atattggtcg
    53161 tttgttaatg ctctgtgttt ggggattttt aatcctcaac ctggtgcatc ccttcccgcg
    53221 cccgctgaat attttcgtta acgtggcgct gatttttacc gtgctgatgc atggtatgca
    53281 actggcgcta ttgaaatcca ctttaccgaa agatggcccg cagatgacca ccgccgaaaa
    53341 ggtgcggatt ttccttttcg gcgtgtttga actgctggtc tggcagaaga aatttaaagt
    53401 taaaaaataa ccttattgtt cgccaacaaa gctaacaaag cgcgttcctt tatagctcag
    53461 cgtaccttta tcgcccactg tcagggcgtg gtactgctgg gcgtcgaggc gaaacgtctg
    53521 ctccattcct ccgctttgcg gtttgaagct tgcctcatag cgcatactgg tgcctgccgg
    53581 agtcacttcc tgctggcgcg aacggcgatc gttaatcggt ttttcccgct tgttgctcac
    53641 caacaccagc ttttgctgga gtggtgccat atcattatca gctttttccc gtcgctgttg
    53701 cataaaacga aacgatgcgg cgacgatgat taagccaatg ataacaataa agaaaagagg
    53761 tggtttgctc atctttatcc ctcatcggaa aatgcggaaa taagcatacc ctgccagtta
    53821 tggtgttgtc atccgtccac cctcgccact aaactggaag caagaccgta ggcattccgc
    53881 ttacgaaaaa ataacgaatt caaggaacta agatgctttg gtcgtttatc gctgtctgtc
    53941 tttccgcatg gctatctgtg gatgcatcgt atcgtgggcc aacctggcaa cgctgggtgt
    54001 ttaaaccgtt aacccttctt ctcctgctgt tactggcctg gcaagcgccg atgttcgacg
    54061 ccattagcta tctggtgctg gcagggctgt gcgcctcact gctgggcgat gcgctaaccc
    54121 tgttgccacg tcaacgtctg atgtacgcca tcggcgcgtt tttcctctcg cacctgctgt
    54181 acaccatcta tttcgccagt cagatgacgc tctctttctt ctggcctctg ccactggtgt
    54241 tgctggttct gggtgcgctg ttactggcga ttatctggac gcggctggaa gagtatcgtt
    54301 ggcctatctg cacgtttatc ggcatgacgc tggtgatggt gtggctggca ggtgaactgt
    54361 ggttcttccg tccgaccgct ccggcactct ctgcgtttgt cggcgcttcg ttgctgttta
    54421 tcagtaactt tgtctggctg ggtagtcact atcgccgacg cttccgtgcg gacaacgcga
    54481 ttgctgcggc ctgctacttt gccggtcact tcctcatcgt ccgctcgctg tatctctgat
    54541 aaaacttgac tctggagtcg actccagagt gtatccttcg gttaatgaga aaaaacttaa
    54601 ccggaggatg ccatgtcgac tcctgacaat cacggcaaga aagcccctca atttgctgcg
    54661 ttcaaaccgc taaccacggt acagaacgcc aacgactgtt gctgcgacgg cgcatgttcc
    54721 agcacgccaa ctctctctga aaacgtctcc ggcacccgct atagctggaa agtcagcggc
    54781 atggactgcg ccgcctgtgc gcgcaaggta gaaaatgccg tgcgccagct tgcaggcgtg
    54841 aatcaggtac aggtgttgtt cgccaccgaa aaactggtgg ttgatgccga caatgacatc
    54901 cgtgcacaag ttgaatctgc ggtgcaaaaa gcgggctatt ccctgcgcga tgagcaggcc
    54961 gccgacgagc cacaagcatc gcgcctgaaa gagaatctgc cgctgattac gcttatcgtg
    55021 atgatggcaa tcagctgggg tttggagcaa tttaatcatc cgttcgggca actggcgttt
    55081 atcgcgacca cgctggttgg gctgtacccg attgctcgtc aggcattacg gctgatcaaa
    55141 tccggcagct acttcgccat tgaaacctta atgagcgtag ccgctattgg tgcactgttt
    55201 attggcgcaa cggctgaagc tgcgatggtg ttgctgctgt ttttgattgg tgaacgactg
    55261 gaaggctggg ccgccagccg cgcgcgtcaa ggggtcagcg cgttaatggc gctgaaacca
    55321 gaaaccgcca cgcgcctgcg taacggtgag cgggaagagg tggcgattaa cagcctgcgc
    55381 cctggcgatg tgattgaagt cgccgcaggt ggacgtttgc ctgccgacgg taaactgctc
    55441 tcaccgtttg ccagttttga tgaaagcgcc ctgaccggcg aatctattcc ggtggagcgc
    55501 gcaacgggcg ataaagttcc tgcaggagcc accagcgtag accgtctggt aacgctggaa
    55561 gtgctgtcag aaccgggtgc cagcgccatt gaccggattc tgaaactgat cgaagaagcc
    55621 gaagagcgtc gcgcacccat tgagcggttt atcgaccgtt tcagccgtat ttacacgccc
    55681 gcgattatgg ccgtcgctct gctggtgacg ctggtgccac cgctgctgtt tgccaccagc
    55741 tggcaggagt ggatttataa agggctgacg ctgctgctga ttggctgccc gtgtgcgtta
    55801 gttatctcaa cgcctgcggc gattacctcc gggctggcgg cggcagcgcg tcgtggggcg
    55861 ttgattaaag gcggagcggc gctggaacag ctgggtcgtg ttacccaggt ggcgtttgat
    55921 aaaaccggta cgctgaccgt cggtaaaccg cgcgttaccg cgattcatcc ggcaacgggt
    55981 attagtgaat ctgaactgct gacactggcg gcggcggtcg agcaaggcgc gacgcatcca
    56041 ctggcgcagg ccatcgtgcg cgaagcacag gttgctgcac tcgccattcc cgccgccgaa
    56101 tcacagcggg cgctggtcgg gtctggcatt gaagcgcagg ttaacggtga gcgcgtgttg
    56161 atatgcgctg ccgggaaaca tcccgctgat gcatttgctg gtttgattaa tgaactggaa
    56221 agcgccgggc aaacggttgt gctggtagta cgtaatgatg acgtgctggg tatcattgca
    56281 ttgcaggata ccctgcgcgc cgatgctgca actgccatca gtgaactgaa cgcgctgggc
    56341 gtcaaagggg tgatcctcac cggcgataat ccacgcgcag cggcggcaat tgccggggag
    56401 ctggggctgg agtttaaagc gggcctgttg ccggaagata aagtcaaagc ggtgaccgag
    56461 ctgaatcaac atgcgccgct ggcgatggtc ggtgacggta ttaacgacgc gccagcgatg
    56521 aaagctgccg ccatcgggat tgcaatgggc agcggcacag acgtggcgct ggaaaccgcc
    56581 gacgcagcat taacccataa ccacctgcgc ggtctggtgc aaatgattga actggcacgc
    56641 gccactcacg ccaatatccg ccagaacatc actattgcgc tggggctgaa agggatcttc
    56701 ctcgtcacca cgctgttagg gatgaccgga ctatggctgg cagtgctggc agatacgggt
    56761 gcgacggtgc tggtgacagc gaatgcgtta agattgttgc gcaggagata aggcaaaccg
    56821 atcgcaacat tgagcgcgat cggtcccctc gctcctctgg ggagagggtt agggtgaggg
    56881 gaaaaggcgg aatcgaagcc aatctgcccc cgtcaaccgc ctttacgaat caaataacga
    56941 taaggcagtc catccgtctc tttggcaacc agttcgtgtt ccataaaggt acaaaaccca
    57001 gggatatcgc gggtcgtcgc cggatcgtca gcaataatca gcaatgtttc acctggctgc
    57061 atattgcgca ctgttttgcg caccatcatg actggttccg ggcagcgcag gccaagtgcg
    57121 tcgagtgtgt ggtcagggct ggaaaagaga tcggtcattt tcttctcatc acataaaaaa
    57181 acggcgctag tttacgccct gtgagtccgt aagcaaacca ggttaacgat tgcgtgaaaa
    57241 ttaaccattg cattgtcaac gtaaagcagt atcatgcggc ggctcgaaaa aagggtaagc
    57301 acgttattat gttaaggtaa cagacgtgtc gtacgtattg ggttccctca ccccaatggt
    57361 taatcaaaaa ggtacaatat gaacgttttc tcgcaaactc aacgctataa ggcgttgttc
    57421 tggttatcgt tatttcatct gctggtgatc acctccagta actatctggt tcagcttccc
    57481 gtctccattt ttggtttcca taccacctgg ggcgcgttta gctttccgtt tatctttctc
    57541 gctaccgacc tgaccgtacg tatttttggc gcaccgctgg cccgtcgtat catctttgcg
    57601 gtgatgatcc cggcattgct gatctcctac gtcatctcgt cgctattcta tatgggttca
    57661 tggcagggat tcggcgcact cgcccacttc aacctgtttg tcgcccgtat cgccaccgcc
    57721 agtttcatgg cctacgcgct gggacagatc ctcgacgtgc acgtttttaa ccgcctgcgt
    57781 cagagtcgcc gctggtggct ggcaccgaca gcgtccacac tgttcggtaa cgtcagcgac
    57841 acgctggcct ttttcttcat tgccttctgg cgtagcccgg atgcctttat ggctgaacac
    57901 tggatggaaa tcgcgctggt cgattactgt ttcaaagtgt taatcagtat cgttttcttc
    57961 ctgccaatgt atggcgtatt actcaatatg ctgttgaaaa gactggcaga taaatccgaa
    58021 atcaacgctt tgcaggcgag ttaaaggttc gttatcagag ttgtgataag atggatgaat
    58081 gagccgttat ggccgtttat cgaaaggaag aagtcaatgc gcaatctggt taaatatgtc
    58141 ggaattggcc tgctggttat ggggcttgcg gcctgtgatg ataaagacac taacgctacg
    58201 gcgcagggtt cggtcgcgga aagtaacgct accgggaatc ccgtcaacct gcttgatggc
    58261 aagttaagtt tctcgctgcc agcggatatg accgaccaga gcggtaagct gggaacgcag
    58321 gccaataaca tgcatgtctg gtccgacgcc accgggcaga aagcagtcat cgtcatcatg
    58381 ggcgatgatc cgaaagaaga tctggcggtg ctggcgaagc gtctggaaga tcagcaacgt
    58441 agccgcgatc cacagctgca agtggtcacc aataaagcca ttgagctgaa aggtcacaaa
    58501 atgcagcagt tagacagtat tatctccgcg aaaggccaga cggcgtactc ttccgttatt
    58561 ctgggtaacg tgggtaatca actgctgacc atgcaaatta ctctgccagc tgacgatcag
    58621 caaaaagcgc agaccaccgc agaaaacatc attaatacgc tggttattca gtaagtttta
    58681 agatgatgag gcggcctcag ggacgtgttc cggaggccgt ttttttaatc gccacgtcag
    58741 taataacgcg attgcgacca gtcccgccgc cgccagataa atcaccggta cgcccgccca
    58801 actcatcacc agcccagcca gtggtccggt cacgccaagc gataaatcca taaatacggt
    58861 gtaggttgcc agcgccgccc cctgattttg ctgcggaacc gcttttaccg ccactacacc
    58921 caatgccggg aacaccagcg aaaacccggc ccccgccagt aagacgccga ttttcgccat
    58981 ccacggcata gtcgccacgc caaccagtag caggccgatt atctcaacgc taaagcaaat
    59041 catcgctacg tttaagccgc cgatacggtt aatgccgtta gggaataaca aacgcgtacc
    59101 gacaaacgca cagctaaaca gcgtcagcgc gaaagccgca ccgtcccaac ctttaacgtc
    59161 ataaaacagc gtgataaagg tggcgatgac gccaaatccg gcggaagcca gtgccagcgc
    59221 cataccgtac agccagacgc gcccaagcac cgcgcgaaac ggcagcggtt tgcctttact
    59281 ggcttttacc gtcggacgcg ggatcgccaa caaaatggcc accagcgcca cgcccataat
    59341 aattaacgct aacgcctgca agccgcccca gtgataaaac acgacgccta acggcgcacc
    59401 catcgccatc gccccgtaag tgacaatgcc gttccacgaa atcacccgcc cgatatgcag
    59461 cgagccaacc acgccaacac cccacagggt cgatcccgtt ccggcaaaac tttgcccaat
    59521 cccaaggatg acgcgtccca ggcaaagtaa taacaggctg atgacgggca gactggcggt
    59581 taatcctgcc gtcagatatc ccagaccgct caaaaagcag ccgcataaac cgaagacgac
    59641 aatctttttg gggcccagca aatcggcgta acgtccggca tgaggacggc tcagcaaggt
    59701 ggcgaaatat tgcaggctga taaccaatcc tgcccagaaa gcgctaaagc ccatcacatc
    59761 atggacatag cccggtaata cagcgagcgg caacccgatg gtgaggtagc tggcgaagtt
    59821 aaacatgaca atggagagaa tgcgcaaatt caggcgcaat ccgtttagcg cgggttcggc
    59881 tacgggttcg ggcatgagga tcaccacatt tttacaacag tgtttcattt ttaccacgtg
    59941 ctgacgtgaa aatcagcagt aagaatcaga atattgccgt cgtgactccc gctacactta
    60001 atacaaaaag tcacaaggaa gccccaatgg aaacccctca acccgataaa acgggcatgc
    60061 acattctgct caagctggcc tcgctggtag tgatcctcgc gggcattcac gcagcggcag
    60121 atatcattgt gcagctgtta ctggcgctgt tttttgccat cgtcctcaac ccgctcgtca
    60181 cctggtttat tcgtcgggga gtacaacgcc ccgttgccat tacgattgta gtggtggtga
    60241 tgctgatcgc actaaccgcg ctggtcggcg tactggcggc atcgtttaac gaatttatct
    60301 ctatgctgcc gaagtttaat aaggagctga cgcgcaaact ttttaaattg caggagatgt
    60361 tgccttttct taatttgcat atgtcgccgg agcgaatgct gcagcggatg gactcggaaa
    60421 aagtggttac cttcaccaca gcgctaatga ccgggctttc cggggcaatg gcgagcgtgc
    60481 ttttgctggt gatgaccgta gtttttatgc tgtttgaagt gcgccacgtc ccttacaaaa
    60541 tgcgttttgc gctgaataac ccacagattc acatcgcggg attacaccgc gcacttaaag
    60601 gcgtttcgca ttatctggcg ttaaagacgc tactcagttt atggacgggt gtcatcgtct
    60661 ggctggggct ggagctgatg ggggtacagt ttgcgctgat gtgggcagta ctggcgtttt
    60721 tgcttaacta cgtgcccaat atcggcgcgg taatttccgc cgtaccgcca atgattcagg
    60781 tgctgctgtt taatggtgtt tacgaatgta ttctggtcgg cgcattgttt ttagtggtcc
    60841 atatggtcat cggcaatatt ttagaaccac ggatgatggg ccatcgcctg gggatgtcca
    60901 ccatggtggt atttctttca ttgttaattt ggggatggct gctcggcccg gtagggatgc
    60961 tactttcggt gccattaacc agcgtgtgta aaatctggat ggaaacgacg aaaggcggca
    61021 gcaaactggc gattttactg gggccgggca gaccgaaaag tcggttaccg ggttaatagc
    61081 acctcctgag caggcaatgg ccaccgttta tctaacagtg gccatttgat ttgaggaaat
    61141 gatgaaacat tattcagaaa attaatttaa aaaaaccatt ccttaagtca aatatatata
    61201 aacgccgaat agcgattaaa tccttctcaa agaatgcact gataacattg tgttattttt
    61261 attgcctcat tttcctcact cacttatagt tggtccctgc tggcagtaat ttagtcattt
    61321 cagagatttt tactctgatg tgcacttact gacttatctc acaagtaatt ttttctctaa
    61381 ataaagaata gtgatgccag caattacagt taaaacttgc tttactcgta aaaacaatac
    61441 tttaatgggg ttctaatgag acattacatg aaatggtttg cggctattgc tgttgtgggt
    61501 gctttagcag gttgcgcgcg tactgcgcca attgatcagg ttcactcaac cgtcagcgct
    61561 ggtcataccc aggaacaagt gaaaaaagcg attctgaaag ctggcgttga acgcaaatgg
    61621 attatgtccg aagccggaca aggcataatt aaagcacgtc aacagtcacg cgatcattca
    61681 gcggaaatac gcattaacta cacggcatcc agctacgata tcaattatga aaatagccag
    61741 aacctacaag cgtcaggcgg acaaattcat aaaaactaca atcgctgggt ccgcaatctc
    61801 gacaaagata ttcaactgaa tttgtcagcg ggcgcagatt tataattctt cctaaagtcg
    61861 ggccgttgcg gtccgataat aatttaattc cctcttaaca ggttgtgccc aacatgtacg
    61921 aacaggattc tcttagcgca ctggatgcga ttaccgaagc tcaacgcatt gcctttgccc
    61981 ctatgctttt tcaaacagca ctctgcctac gtaatgcggg tgtactttca tatctggatc
    62041 ggcaaggcaa acatggcgca acgctggctg atatcaccga acatagccat atcaacgaat
    62101 atgctaccag cgttctgtta gatatgggct taagcgggcg aattattacc tgtaaagagg
    62161 gaatttatta cctggcgaaa atcggtcatt acctcctgca tgacactatg actcgcgtga
    62221 atatggattt cacccaggat gtctgttacc aggggctctt cttcctcgcc gattcactca
    62281 atgaaggaaa accttccggg ttaaaagtat ttggcgactg gtcgacgata tatccggcac
    62341 tttcgcaatt accagacgcc gctcgtgaaa gctggtttgc gtttgatcat tactattcag
    62401 atggcgcatt taacgccgca ctgccttatg tattcgccaa caaccccaca acattgtacg
    62461 atgtaggcgg caatacgggt aagtgggctt tgcgttgctg caaatacaat gaaaacatcg
    62521 ccgttacgtt attagatctg ccacaacaaa tcgtactagc gaaagaaaat atcgcgaatg
    62581 caggtttttc tgatcgcata gatttccatg cggtggatat gctaagcgat gccccattac
    62641 cgggcgaggc tgatatctgg tggatgagcc aatttttaga ctgtttttca cccgaacaaa
    62701 taattagcat actcagcaaa attgccagcg taatgaagcc tggcgcaaaa gtttgcatca
    62761 tggagttgtt ctgggatgcg cagcggtttg aagcagcatc gtttagcctg aacgcgtcct
    62821 cactttattt tacctgcatg gcaaacggta acagccgttt ctatagtgca gagaaatttt
    62881 acgactatct gaataaagca ggattccagg tagcagagcg ccacgataat ttaggcgttg
    62941 ggcatacact tttgatatgc caaaagaaat aatcgtcact taataatttt ggtttaccgc
    63001 gggcgcgcgt tgatgaaatt tgcattaaac attctcgact ggcaggcaag agcgcctgga
    63061 cttagtgaaa cgctgcaatg gcaggagtgg tcacgtcagc ctcaggcaat tgacccgaca
    63121 gcaccgttgg caaaattaag tgaactgcca atgatgaccg cccgccgcct cagctccggg
    63181 agcaaactgg ctgttgaatg cggtctggca atgctacgcc gccatcaacc agacgccgtt
    63241 ttgtatacca gtcgtcatgg cgaactggag cgcaattacc gcattgttca tgcactggca
    63301 accgaacagg cgctttctcc gacggacttc gcgctctccg tacacaactc ttccgtgggt
    63361 aacctgacca ttgcggccaa acagccgagc gtttcgtcat cgctttcggc tggtcgcgat
    63421 agtttccagc aaggtttgtg tgaagtgctt agcctgctac aagcgggata ccagcgcgtg
    63481 ctgatggtcg attttgacgg tttcctgccc gagttttatc acccacaact gccatcagaa
    63541 atgccgacct ggccgtacgc cgtcgcgctg gtgattgaag ctggcgatga ctggcaatgt
    63601 gaaacgcagt cagccattgc gggcaatgaa acaacactgc cgcaaagcat gttgttttta
    63661 cagcactatt tacaaaatgt agacacattt tcactgcctg gcgagcgcgt gcaatggcgc
    63721 tggagccaca gatgagtaag ctgatgtcac gtctggagtg gacctggcga ctggtcatga
    63781 ccgggttatg ttttgccctg tttggtctcg gcggcctgct gctttctgtc gtgtggttta
    63841 acatattgtt agttttggtg tgggatactt cccgccgccg tcgtttggcg cgtcgcagca
    63901 ttgccgccag ttttcgcctg tttttaactg tcgcgaaagg gcttggcgta ctggattatc
    63961 gtattgatgg cgctgaaatc ttacatcagg aacgcggttg cctcgtcgta gccaatcatc
    64021 ccacgctgat cgattacgtg ctgctggcgt cagtcatgcc agaaactgac tgcctggtga
    64081 aaagcgcgct gttaaaaaat ccttttcttg gcggcgtcgt gcgggcgaca gattatttaa
    64141 tcaacagcga agctgaaact ctgttacccc gctgccaaca gcgactggca cagggtgaca
    64201 ctatcctgat tttccccgaa ggcacacgaa cgaagcccgg tgaaaaaatg acgctgcagc
    64261 gcggggcggc gaatattgcc gtgcgctgcg ccagcgattt acgcatcgtg acaatcggct
    64321 gtagcgagca tctgctggat aaacaaagta aatggtatga cgttccacct gaccggccgt
    64381 tttttacggt tgcagtccat ggtcgggttg aaatcaataa attttacgat gcaacttcac
    64441 aagaaccggc gctggcagca aggcagttaa accggcatct tttgcttcaa ttacaacaag
    64501 gttgtttacc tctctcagga ataaataatg caagcgcttt atctggaaat taaaaacctc
    64561 atcatcagca ccctcaatct ggacgagttg acgcctgatg atatcgatac cgacgcgccg
    64621 ctgtttggcg acggtttagg gctggactct atcgatgccc tggaactggg actggcagta
    64681 aaaaatgagt acggcatcgt gctctctgcg gaaagcgaag agatgcgtca gcactttttc
    64741 tccgtcgcca ctctggcctc tttcattgct gcgcaacgtg cctgaggaaa gccatcatga
    64801 cagaacaaca aaccgtctat caggaagtct cagccctgct ggttaagctg tttgaaatcg
    64861 atccgcagga catcaaacct gaaacgcgcc tgtacgaaga tctggaactg gacagcatcg
    64921 acgccgttga catgattgtg cacctgcaaa agaaaaccgg taagaaaatc aagccggaag
    64981 agtttaaagc agtgcgtaca gtccaggatg tcgtcgaggc tgtagaacgc ctgctacaag
    65041 aagcctgatc tgatgtcggg cattcgctcg ctaccgatga ttaagctgct gactggatta
    65101 ttgctgctcg cctggccgtt tgtgatttgg tttggcttag cgcataacgg cctgcactgg
    65161 ctgttaccgc taatggcgtt gctgcttttg ttgcgtctgc gccagacccg tcgccaggcg
    65221 gggcctttac aagccgtcac gcaactcgtc gctgtcgtgg gcatcgcatt atgtgtcgcc
    65281 agctttatgt tgaaaacgca ccagctactg ctgttttacc cggtagtggt taacgcggtc
    65341 atgctggcgg tttttggcgg ttcgctgtgg tcggcaatgc cgatagttga gcggctggcg
    65401 cggttgcagg agccagacct cccggaaaag ggcgtgcgct atacccgcca cgtcacgcaa
    65461 atctggtgcg gctttttcat cattaacggt ggaattgccc tctttaccgc gctttatgcc
    65521 gatatgtcgt tatggaccgc ctggaacggc atgattgctt atttgttgat ggggacgtta
    65581 atggctggtg aatggctgct gcgccgtcag gtgatgaaaa gagatcgcgc atgaagcaga
    65641 ccttaccgct tgcacgctgg ctgactgcgc cacgccctga tgacacaccc attgcgtggc
    65701 tgaacgaaag tacctggacg ctgggcgatt tgcgtcacga tgtcgcgcaa cttatctgcc
    65761 gcttgcagca acagccgggt gaacgctggg cgctgtgctt tgagaacagc tatctgttta
    65821 tcgttgccct gctcgccacg ctacacgccg gaaaaacgcc ggtgcttccg gggcataacc
    65881 gtgttatcca gcttaacgag caacgtgaac tttttgatgg tgtgcttagc gacagcgaac
    65941 ttaactggca aggttcattg ctgttggtag caagttctcc acaaatcgcg acacaatcct
    66001 tcacctttgc cgctatagcg ccagaggctt ttatcgagct gtttacctcc ggttccacgg
    66061 gacagccgaa acgggcgatt aaacctgttc atttgctgga tcgtgaagca gaactgctgg
    66121 ctgaacgatt aggcgcacgt ctggcgggtt gtcgtgtcgt cggttccgta ttgccgcagc
    66181 atttgtacgg cctgactttt cgcgttttcc tgcccatggc gttgggatta ccgctgcacg
    66241 ccgccatgct ctggtatgtc gaacagtttg ccgcgttgag tcatcagcat cgctatatct
    66301 tcatcagcag cccggcattt ttgaagcgcc tggataccca gctttctccg ccacccgttc
    66361 aggtgcttct ctctgctggc gctggtgctg gtgcttggca tcggtatcaa ctacacgctg
    66421 tttttcagta atccgcgcgg tacaccgtta acttcgctac tggcgatcgc gctggcaatg
    66481 ctcaccacct tgctgacgct gggtatgctg gtattcagcg ccacccaggc catcagcagt
    66541 tttggcattg tgctggtgag cggtattttc accgccttcc tgctttcgcc gctggctatg
    66601 cccgataaaa agagaacaaa aaaatgatca aatccacatt ctggcgagcg ttcgccctga
    66661 ccgctacgct tatcctcacc ggctgtagcc actcgcaacc agaacaggaa ggccgcccgc
    66721 aggcgtggct gcaacctggc acgcgcatca cgctgccggc accagggatt tcacccgcag
    66781 tcaattcgca gcaactgttg accggcagct tcaacggcaa aacccagtcg ctgttagtga
    66841 tgcttaacgc cgacgatcag aaaatcaccc ttgccgggct gtcgtcggtc ggcattcgcc
    66901 tgtttctggt gacctacgat gcgcaagggc tacgcgccga gcaatccatc gtcgtcccac
    66961 agttaccgcc cgcaagtcag gtactggctg acgtgatgct cagccactgg ccgattagcg
    67021 cctggcaacc gcaacttccc gcaggatgga cacttcgcga caacggtgac aaacgcgagc
    67081 tgcgtaacgc cagcggcaaa ctggtcacgg aaatcaccta tctgaatcgc aagggcaaac
    67141 gcgtgccaat cagcattgag cagcatgtct ttaaatacca catcaccatt caatacttag
    67201 gtgactgata tgatttatat ttccgccgtc ggcatgatca acgccctggg aaataacctt
    67261 gacgaaatcg caaccaacct gactcgcggt gttgctcccg gcatgcgccc gcgcgcaggc
    67321 tggttgcagg ggcatccgca ggcggtcctg gctggcgtgg atggcgagct tccgcttatc
    67381 ccggaaaaat tcgctgcgca ccgctcgcgc aacaatcaga tcctgctggc ggcgctggcg
    67441 cagatccagc cgcaggttga tgacgccatt gcgaaatatg gccgcgagcg cattgcgatt
    67501 gtgcttggta ccagcacctc cggcctgcat gaaggcgaca cgcacgttaa cctgcgcacc
    67561 cacgggcagc cgagcaccac atggcactac gcgcaacagg aactcggcga tccgtcgcgt
    67621 tttctcagcc actggctggc gctcgacggc ccggcctata ccctttcaac cgcctgctct
    67681 tccagcgcca gagcgataat cagcgggcgc aggcttatcg aagcggggct ggtcgatgcc
    67741 gccattgtgg gtggcgcaga caccctgagc cggatgccga ttaacggttt tcacagcctc
    67801 gaatcgctgt cgccaacctt atgtcagccg tttggtcgtg accgcgcagg tatcaccatt
    67861 ggtgaagggg cggggctaat gctgctgacc cgggaaccgc agcccatcgc gttgctgggc
    67921 gttggtgaat cgagcgacgc ttaccatatt tcagccccgc atccgcaggg cgaaggagcg
    67981 atccgcgcta ttaatcaggc gctgaccgat gcgcagctta cgccagatga cgtcggctat
    68041 atcaacctgc acggcaccgc cacacaactc aacgatcaga ttgaatcaat ggtggttaac
    68101 gccctgtttg gcgaacgcgt accgtgcagc tccaccaaac atttgaccgg acacacgctg
    68161 ggcacagcgg gcattaccga ggcggcaatt agtatgttga ttttacagcg tgatttaccg
    68221 ctgcccgctc aggacttcag cctgtcgccg cgcgatccca cgctgccacc gtgtggcatt
    68281 atcgaaaaac cgcagccgct ggcacgtccg gtgatcctat ccaattcctt cgcctttggc
    68341 ggcaataacg ccagcattct gctcgggagg gtttcgtgag ccactattta tcccccggcg
    68401 cttatctgcc ccacgacgcg cccatgttac tgctggaaga cgtggtgagc gtcagcgatg
    68461 acagcgcggt gtgccgcgtg acggtttcgc ccagtggcgt actggcaccg tttctcgacc
    68521 cggacggcaa tctccccggc tggtttaccc ttgaactgat ggcgcaaacc gtcggcgtct
    68581 ggtctggctg gcatcgccac cagcagggca aaaacagtat tgagttaggg atgatactgg
    68641 gcgcgcgtga attgctttgc accactggca ttctgcccgc aggccagaca ctgaccatta
    68701 ccgtcaaact gctgatgcaa gacgatcgct tcggcagttt tgagtgcagc atcaacgacg
    68761 gcgaggcgac gggccgcatt aacaccttcc agccgaccgc ggaagaactt accaccctgt
    68821 ttcaacaagg agcgtcctga tgagtcgttc agttctggtt actggtgcca gcaaaggcat
    68881 tggtcgcgcc atcgcctgcc aactggcggc agacggcttt aacattggcg ttcactatca
    68941 ccgtgacgcc acaggcgctc aggaaacgct gaatgccatt gtcgccaatg ggggtaacgg
    69001 gcgtttgctc agtttcgacg tcgcaaaccg cgaacagtgt cgggaagtgc tggagcacga
    69061 aatcgcgcaa cacggtgcct ggtacggcgt ggtcagtaac gccgggatcg cccgcgatgc
    69121 cgcttttccg gctttaagcg atgacgactg ggatgcggtg atccacacca acctcgacag
    69181 tttctacaac gtcattcagc cgtgcattat gccgatgatc ggcgcacgcc agggtgggcg
    69241 catcatcacg ctatcctcgg tttccggcgt aatgggcaat cgcggccagg tgaattacag
    69301 cgctgccaaa gccggaatta tcggcgcaac aaaagcgctg gctatcgaac tcgcgaagcg
    69361 caaaatcacc gtcaactgca tcgcgccggg gcttattgat accgggatga tcgaaatgga
    69421 agagagcgcc ctgaaagagg caatgtcgat gatccccatg aaacgcatgg gccaggcgga
    69481 agaagttgcc gggcttgcca gctatttaat gtctgatatt gcgggttacg tcacccgcca
    69541 ggttatttcc atcaacggag ggatgttatg acacgtcgcg tagtgattac aggaatgggc
    69601 ggcgtgactg cctttggtga aaactggcag gacgtttctg cacgcttact ggcatatgaa
    69661 aatgcggtgc gcaaaatgcc ggagtggcag gtctacgatg ggctgcacac cctgcttggc
    69721 gcgcctgttg atgatttcac gctaccggag cactacaccc gcaagcgtat ccgtgcgatg
    69781 ggccgcgtgt cgcaaatgtc gacccgtgcc agcgagctgg cgttagagca ggcagggttg
    69841 attggcgatc cgatattaac cagcggcgaa acgggcattg cgtacggttc atcgaccggc
    69901 agtaccggcc cggtaagcga gttcgccacc atgctgacgg aaaagcacac caataacatc
    69961 actggcacca cctatgtgca gatgatgccg cacaccaccg ccgttaatac tggcctcttt
    70021 ttcggcctgc gcgggcgggt gatcccaacc tccagcgcct gtacttccgg cagccaggca
    70081 atcggttatg cgtgggaagc cattcgccac ggttatcaaa cggtgatggt tgctggtggt
    70141 gccgaagagt tatgtccttc agaagcagcg gtatttgata cgcttttcgc caccagccag
    70201 cacaatgatg cgccgaaaac cacaccttca ccgttcgatg aaaaccgcga cgggctggtg
    70261 attggtgaag gcgcaggtac gctcattctc gaagaactgg agcacgccaa agcgcgcggc
    70321 gcgaccattt acggtgaaat cgtcggcttt gcgactaact gcgacgcggc acatattacc
    70381 cagccgcagc gcgaaaccat gcagtattgt atggagcaat cgctgaaaat cgccgggtta
    70441 agcgcacaag atatcggcta tatttcagcg catggtacgg cgaccgatcg cggtgatatg
    70501 gcagaaagtc tggcgacagc aaccatttac ggtgataatg tgccgctctc ctcgctgaaa
    70561 agttattttg gtcatacgct tggtgcctgt ggcgcgctcg aagcctggat gagtctgcaa
    70621 atgatgcgcg aaggttggtt tgcgcctacg ctaaacctga ataaaccaga cccgaattgt
    70681 ggcgcgctgg attacatcat gcatgaagcc cgcaaggttg attgtgagtt tttgcagagt
    70741 aacaactttg cctttggcgg cattaataca tccatcatca ttaaacgttg gccctgatat
    70801 gtatcggata gttctgggga aagtttcgac cttaagcgca gctccactgc caccgggttt
    70861 acgcgagcaa gcaccgcaag gtccacgacg cgaacgctgg ctggcggggc gtgcattgct
    70921 ttcgcacacg ctttccccgc taccggagat catctatggc gaacaaggca aacctgcatt
    70981 tgcgccggaa acgccgctat ggttcaactt aagccatagc ggtgacgata tcgccctgct
    71041 gttgagtgat gaaggcgaag tcggctgcga tatcgaagtg attcgcccgc gcgccaactg
    71101 gcgctggctg gcgaacgccg tgttcagcct cggggaacat gctgagatgg acgccgtgca
    71161 tcctgatcag caactggaaa tgttctggcg catctggacg cgcaaagaag ccatcgttaa
    71221 acagcgtggt ggcagcgcct ggcaaatcgt cagcgtagac agcacctatc actcctcgct
    71281 gtcagtcagc cattgtcagc tagaaaattt aagcctggcg atctgcaccc ctactccctt
    71341 tacgctcacc gccgacagtg tgcaatggat cgattcagtt aactgatccg cccacccgac
    71401 tgcccatcta ttgatccaga acaggtaatc agtatgacga atacttaaaa tcgtcatact
    71461 tatttccgcc atctatttta atccattggg gttaccatgc tctccacact ccgccgcact
    71521 ctatttgcgc tgctggcttg tgcgtctttt atcgtccatg ccgctgcacc agatgaaatc
    71581 accaccgcct ggccggtgaa tgtcgggcca ctaaacccgc acctttacac gcctaaccag
    71641 atgttcgccc agagcatggt ttatgaacca ttggtgaaat atcaggcaga cggttcggtg
    71701 atcccgtggc tggcaaaaag ctggactcat tcagaagatg gtaaaacctg gaccttcacc
    71761 ctgcatgatg acgtgaaatt ctctaacggc gaaccgtttg atgcagaggc ggcggcagaa
    71821 aacttccgcg cagtgctcga taaccgtcaa cgtcacgcct ggctggagct ggcaaaccag
    71881 attgttgatg ttaaagcact cagtaaaaca gagctgcaaa tcaccctgaa aagcgcctac
    71941 tatcctttcc tgcaagaact ggccctgccg cgccctttcc gctttatcgc cccctcgcag
    72001 tttaaaaacc atgaaaccat gaacgggatt aaagcgccga ttggcaccgg accgtgggtt
    72061 ttgcaggaat cgaaactgaa tcagtacgat gtcttcgtcc gtaacgaaaa ctactggggc
    72121 gaaaagccgg caattaagaa gatcactttt aacgtcatcc cagacccgac tacccgcgcg
    72181 gtggcgtttg aaactggcga tatcgacctg ctgtacggaa acgaagggtt attaccgctc
    72241 gataccttcg cccgctttag ccagaacccg gcttaccata cccaactgtc acagccgatc
    72301 gaaaccgtga tgctggcgct taataccgcc aaagccccca ccaatgagct ggcagtacgt
    72361 gaagctctta attacgcggt aaacaaaaaa tcgctgattg ataacgcgtt gtatggcacc
    72421 cagcaggtcg ccgacaccct gtttgcccct tctgtgccct acgccaacct cggcctgaaa
    72481 ccgcgccagt acgatccgca gaaagcgaaa gcgttgctgg aaaaagccgg ttggacgctg
    72541 cctgcgggca aagacatccg cgagaaaaat ggtcagccgc tgcacattga actttcgttc
    72601 atcggcaccg atgcgttaag caaatcgatg gcggaaatca ttcaggctga tatgcgccag
    72661 attggcgcag atgtctcgct gattggcgaa gaagagagca gtatctatgc gcgtcagcgc
    72721 gacggtcgtt ttggcatgat tttccaccgc acctggggcg cgccatacga tccacacgcc
    72781 ttcctgagtt caatgcgcgt accgtcgcac gctgacttcc aggcacagca aggattagcc
    72841 gacaagccgc tgattgataa agagatcggc gaagtgctgg cgacccatga cgaagcacaa
    72901 cgtcaggcac tgtatcgcga cattctgacc cgtctgcatg acgaggcggt ttatctgcct
    72961 atcagttaca tctcaatgat ggtggtatca aaaccggagc tgggtaacat cccctacgcg
    73021 ccgatcgcca ccgaaattcc gttcgaacag attaaaccgg tgaaacctta atgttgcgtt
    73081 acgtattacg ccgctttctg ctgctgatcc cgatggtgct cgccgcctcg gtgatcattt
    73141 ttctgatgct gcgcctcggt accggcgacc cggcgctcga ttatttgcgt ctgtctaacc
    73201 tgccgccgac gccggagatg ctggcctcta cccgcaccat gctgggactg gatcagccgc
    73261 tgtatgtgca gtacggcacc tggttgtgga aggcgctgca tcttgatttc ggcatctcat
    73321 ttgccacgca gcgcccggtg ctggacgata tgctgaactt cctgcccgcc acgctggaac
    73381 tggcgggtgc ggcgctggta ttaattctgc tcacttccgt accgctcggt atctgggcgg
    73441 cgcgccatcg tgaccgtctg ccggatttcg ccgtacgttt catcgcgttt ctcggcgtgt
    73501 cgatgcccaa cttctggctg gcatttttac tggtgatggc gttttcggtg tatctgcaat
    73561 ggctacccgc gatgggctac ggcggctggc agcacatcat tttgcctgcg gtttccattg
    73621 cctttatgtc gctggcgatt aacgcacggc tgctgcgcgc cagtatgctg gacgtcgccg
    73681 gtcagcgtca cgtcacctgg gcgcggctac gcggcctgaa tgacaaacag accgagcgtc
    73741 gtcatattct gcgcaatgcc tcgctgccga tgatcaccgc cgtggggatg cacattggcg
    73801 aactgattgg cgggacgatg attatcgaaa acatctttac ctggcccggc gtcgggcgct
    73861 atgcggtgtc ggcgattttt aaccgtgact atccggtgat ccagtgcttt acgctgatga
    73921 tggtggtggt ttttgtggtc tgtaatttga ttgtcgattt gctcaacgcc gcactggacc
    73981 cacgcattcg tcgtcatgaa ggagcgcacg cgtgaacttt ttcctctctt cccgctggtc
    74041 ggtacgcctg gcgctgatca ttatcgccct gctggcgctg attgcgctca ccagccagtg
    74101 gtggctgccg tatgaccccc aggcgattga tttgccgtcg cgcctgcttt cgccggatgc
    74161 gcagcactgg ttgggcaccg atcacttagg gcgcgatatt ttctcgcggc tgatggcagc
    74221 gacccgtgtg tcgctcggtt cggtaatggc ctgcctgctg ctggtgctga cattagggct
    74281 ggttattggc ggcagcgccg ggctgattgg cgggcgcgtt gatcaggcta ccatgcgcgt
    74341 cgccgatatg tttatgacct tcccgacctc gattctgtca ttctttatgg ttggcgtgct
    74401 cggcactggg ctgaccaacg taattatcgc catcgccctg tcgcactggg cgtggtatgc
    74461 acgcatggtg cgcagcctgg tgatttcact gcgccagcgc gagtttgtgc tggcatcacg
    74521 gctttctggc gcgggccatg tgcgagtgtt tgttgatcat ctggcaggtg cggtgatccc
    74581 ttcgctgctg gtgctggcaa cgctggatat cggccatatg atgctgcacg ttgccgggat
    74641 gtccttcctc ggcctcggcg tgaccgcgcc gaccgccgaa tggggcgtga tgattaacga
    74701 cgcgcgccag tatatctgga cccagccgct gcaaatgttc tggccggggc tggcgctgtt
    74761 tatcagcgtg atggccttta acctggtggg tgacgcactg cgcgatcatc tggatcctca
    74821 tctggtgacg gagcacgcac actaatgccg caacagattg aactacgtaa tatcgcgcta
    74881 caggccgcgc agccgctggt gcacggcgtg tcgttaaccc tgaaacgcgg gcgcgtgctg
    74941 gcgttagtcg gcggtagcgg cagcgggaaa tcattaacct gcgccgcgac gctgggaatt
    75001 ctgcccgcag gcgttcgcca gacggcgggg gaaattttag ccgatggcaa accggtttct
    75061 ccctgcgccc tgcgcggcat caaaattgcc accatcatgc agaacccgcg cagcgccttt
    75121 aatccactgc acaccatgca cacccacgcg cgggagacgt gcctggcgct ggggaaaccc
    75181 gccgatgacg ctacgcttac cgctgccata gaagcggtgg ggctggaaaa cgccgcacgc
    75241 gtgctgaagc tgtacccgtt cgagatgagc ggcggcatgt tgcagcgcat gatgattgcg
    75301 atggcggtgc tttgcgaatc accgtttatc atcgccgatg aaccaaccac cgacctcgac
    75361 gtggtagcac aggcacgcat tctcgatctg ctggaaagca ttatgcaaaa acaggcgccg
    75421 gggatgctgc tggtcaccca tgatatgggc gtggtggcgc gtctggcgga tgacgtagcg
    75481 gtaatgtctg acggtaagat tgtcgaacag ggcgatgtcg aaacgctgtt taacgccccc
    75541 aaacatgcgg taacgcgcag cctggtttcc gctcatctcg ccctctacgg tatggagctg
    75601 gcatcatgac tttacttaac gtctccgacc tttcccatca ctatgcccac ggtgggttta
    75661 gcggaaaaca tcagcaccag gcggtgctga ataacgtttc cctgaccctg aaaagcggtg
    75721 aaaccgtcgc cctgctggga cgcagcggct gcggaaaaag cactctctcg cggctactgg
    75781 taggcttaga atcgccctca cagggaaata ttagctggcg tggcgaatcg ttggcaaagc
    75841 tcaatcgcgc ccagcgtaaa gcgttccgcc gcgatattca gatggtattt caggactcca
    75901 tcagcgccgt gaatccgcgc aaaaccgtgc gcgagatcct gcgggaaccg atgcgccacc
    75961 tgctatcgct gaaaaaatcc gaacaactgg cgcgtgccag cgaaatgctg cacgcggtcg
    76021 atctcgacga cagcgtgctc gacaagcgcc caccgcagtt aagcggcggc cagctccagc
    76081 gcgtctgcct ggcacgcgcg ctggcggtcg aaccgaaatt gctgattctg gatgaagccg
    76141 tttctaacct tgatctcgtg ttacaggcgg gcgtcattcg cctgctgaaa aagctacaac
    76201 aacagtttgg cactgcctgc ctgttcatca ctcatgactt acgcctggtg gaacgctttt
    76261 gccagcgagt gatggtgatg gacaacggac aaatcgtcga aacccaggcg gtgggagaga
    76321 aattaacctt ttcctctgac gccggacgtg tgctacaaaa cgcgatatta cccgcattcc
    76381 ccgtgcgccg tcgcgccaca gaaaaggttt aacgcaaatg caacgagtca ccatcacgct
    76441 tgatgacgat ttactggaga cgctggacag cctgagccag cgtcgtggtt ataacaaccg
    76501 ttccgaagct atccgcgata ttctgcgtag cgccctggcg caggaagcta cccagcagca
    76561 cggcacacaa ggtttcgcgg tgctgtcgta tgtgtatgaa cacgaaaaac gcgacttagc
    76621 cagccgtatt gtttccaccc agcatcatca ccacgacctc tccgtcgcca cgctacatgt
    76681 gcacatcaac cacgacgact gtctggaaat cgccgtgttg aaaggtgaca tgggtgacgt
    76741 gcagcatttt gccgatgacg ttatcgccca gcgcggcgtg cggcacgggc atttgcagtg
    76801 cctgccgaag gaagattaac acctaccaac gcgccgttat ttccttacgg cgcagtaaac
    76861 ttctgacaaa tatcgactca cgtcacattt ttttgatcac gatttggtcc ctcaaaatgt
    76921 gagacgcttc gatcctcttt ttctcccgtt cacctactat acgcttgaag ttgtccatga
    76981 ttgtccatag acaactgtaa atgttcaggt gcgcgccatg aaatcattaa gtaagtcgtc
    77041 acaaataccg ctctatcaac aagtggtgga gtggataaga gaaagtattt ataccggaga
    77101 tctggtggaa gacgatcgca ttccttcgga ataccagatt atggatatgc tggacgtgag
    77161 tcggggaacc gttaaaaaag cggtcgccca actggtaaaa gaaggcgtgt taatacaggt
    77221 ccagggtaag ggaacatttg tcaaaaaaga gaacgtggca tatccgttag gtgaaggatt
    77281 attgtcattc gcggaatcgc tggaaagcca gaaaatacat tttaccactg aagttattac
    77341 gtcacggatt gaatcggcta atcgttatgt ggcagagaaa ttaagaataa cgcccggtca
    77401 ggatattctt taccttgaac gcttacgctc aattggtgat gaaaaagcga tgctgataga
    77461 gaaccgtatc aatattgagc tatgccccgg tattgctgaa atcgatttta atcaacacaa
    77521 tttatttcca acaatagaaa gtttgtcgca aagaaaaatt cgttactcgg aaagtcgcta
    77581 tgccgcgcga ttaattggta atgaacgtgg tcatttttta gatatcagtg aagacgcacc
    77641 cgttttgcat ctggagcagt tagtcttttt ctcccgagga ttacccattg agtttggcaa
    77701 cgtctggtta aaaggaaata aatattatct tggcaccgta ctgcaacggc gggaagtgag
    77761 ttaagtacac ttttttaaga ggaataaaat gcaaggcata cattttcagg aaaattatat
    77821 tcaacgactt ccggcagggt taagcgttga acagatcatc cgccagttag cgcaaccgtt
    77881 ggttgccgcc gagctggtcg tccctgattt tgccgatcac gtactgaaac gtgaggcgac
    77941 gtacccaacg gggctgccta ccgaaccacc gtgcgtcgct attccgcaca cggaccataa
    78001 acatgtcagg cacaatgcaa ttgccgtcgg catcctgccg gaaccggtag agtttgccga
    78061 tatgggcggc gatcctgacc ccgtacctgt cagggtgatt tttttgctcg ccttaagcga
    78121 aagcaataaa caattaaatg ccctgggctg gattatggaa atgattcagg atacgccctt
    78181 tatgcaagcc ctgcttgcga tggaaacaac agaaatacac acagtaatct taaacaaaat
    78241 gaaagaacga ggtgaaatat gagtcaaacc attttatttg tctgcgccac gggtattgcc
    78301 acatccacgg cggtaacaga aaaagtcatg gaatattgca aagagcaagg tcttaacgtc
    78361 aattattccc aaaccaacgt tgcctcttta cctggaaata ctgacggcgt agcactggta
    78421 gtttcaacaa ctaaagtgcc ttatgaactc gacgtcccgg tggtgaacgg cttgccgatt
    78481 attaccggta ttggcgaaga aaaagtactg gcgcaaattg tttctatcct taaaaagtaa
    78541 ttaataataa ttaatttcaa cccactccat caggcgctaa cgcgaggtgc gctctcgcct
    78601 gaaaaagagg tttattatga acgatatcgc gcataccctc tataatattg tgcaatatat
    78661 attgggattt ggcccgacgg taatgttgcc gttggtgtta tttattcttg ctctctgttt
    78721 taaagtaaaa cccgctaaag ccttacgttc gtcattaaca gtcggcattg gttttgtcgg
    78781 tatttatgcc attttcgata ttctgaccag caatgtcggg ccagcggccc aggcgatggt
    78841 tgaacgcacc ggaattaatt taccggtggt agatttaggc tggccgccgc tttccgctat
    78901 tacatggggt tcgccaattg ccccgtttgt tattcccctg accattctga ttaacgtggc
    78961 gatgctggcg ttaaataaaa cccgtaccgt tgacgtagat atgtggaact actggcattt
    79021 tgcccttgct ggtacgttgg tttattacag caccggcagc ctgttctttg gtttgctggc
    79081 ggcggcgatt gctgcggtgg tagtacttaa actcgccgac tggtctgcgc cactggtaca
    79141 aaaatacttt ggactggaag ggatctcatt gccgacgctc tcttcggtgg tgttcttccc
    79201 ggtcggtctg ctggtcgaca aaatcatcga ccatatccct ggcctcaatc gtattcatat
    79261 cgacccggaa accgtacaga aaaagtttgg catcttcggc gaaccgatga tggttggcac
    79321 tattctgggc attctgctcg gcgtaattgc cggatacgat ttcaaaaaag tattgctgct
    79381 tggcatcagc attggcggtg tgatgttcat cctgccacgc atggtacgca tcctgatgga
    79441 aggtttatta ccgctgtctg aagctattaa aaagtatctc aatgccaaat accctgaccg
    79501 tgacgatctc tatatcggcc tggatatcgc cgttgccgta ggtaatccgg cgattatctc
    79561 caccgccctg ctgctaacgc caatctcggt ctttatcgcg tttgtccttc cgggtaatga
    79621 agtcctgccg cttggcgacc ttgccaacct ggcggtaatg gcgtcgatga ttgctttagc
    79681 cagccgtggc aatattttcc gcaccgttct ggcggcgatc ccggtgatta ttgccgacct
    79741 atggattgct accaaaatcg cgccgtttat taccggaatg gcgaaagacg ttaacttcaa
    79801 atttgccgaa ggctccagcg gccaggtttc cagttttctt gatggcggta acccgttccg
    79861 cttctggctg ctggaaatct tcaacggcaa tctcatcgcc attggtctgg tgccggttat
    79921 cgccctggta ctgtatggca ttttccgaat gacgcggagc acggtttatg cctgataaca
    79981 gcgcagctat cgttatcgat attggcacca ccaattgcaa agtcacctgc ttttcctgcc
    80041 tggacgcaac gacgttgggc gcgcataaat tcgtgacggc aaagcagatc tccccacagg
    80101 gcgatgtcga tttcgatatc gacgccctct ggcaggaggt ccgccaggcg atagcgcaac
    80161 tgaacgccgc ttcgccgctg ccagtcagac ggatcagcat tgccagtttt ggcgaatcag
    80221 gcgtgttcct tgacgagcat ggcgagatcc tgacgccaat gctggcatgg tatgaccgtc
    80281 gcggtgaaga gtatctggca acgcttagcg aggcagacag tgcggcactt tatgacatct
    80341 gcggtctacc actacacagc aattactctg ccttcaaaat gcgctggttg ctggaacatt
    80401 acccgctgcg taatcgccgc ggcctgcgct ggctacatgc gccggaagtg ctgctctggc
    80461 ggctgactgg cgaacagcac acggatatca ccttagccag ccgcacgctg tgtctggacg
    80521 tgcgcaaagg cgaatggtca gcgaaagcgg cggcgttgtt acacgttccc tgtacggcat
    80581 ttgcgccatt ggtgcagcca ggcgagcacg ccggatgggt cagcgagtca ctttgcaaga
    80641 cgcttgggtt ctcgcaaccg gtcagcgtga cgctggccgg acatgaccat atggtgggtg
    80701 cgcgagcgtt gcagatgatg ccaggcgata tccttaactc gacggggacc acggaaggca
    80761 ttctgcaact ggatacacaa ccgacgctgg atgaacaggc caaacgtgac aagctggcaa
    80821 acggctgtta ctcacttgcc aaccagttca ccctgtttgc gtcgctgccc gtgggcggtt
    80881 tcgcgctgga gtggctgcgc aacacgttcc ggctaaccga tgaggagatc gccgcatcac
    80941 ttactcgcgg acatgctgat tatctggcgg ggaattggtt gctcgatgac attcccgtct
    81001 ttattccaca tcttcgcggt tcgggttcgc cctataaaaa tcgccatacc cgtggattat
    81061 tttatgggct tggcgatacg ttaagtatcg acatgttaat tgccagcgta tcactgggat
    81121 taaccatgga atttgccaac tgcttcgcct gttttaacgt gcctggcacc agcgcgttaa
    81181 aagtgatcgg tccggcaacc cataatcctc tttggctgca attaaaggcg gatattttac
    81241 agcgtccggt tgaagcaatt gcatttaacg aggcggtttc tgtcggagca ttattaaccg
    81301 ccgcaccgga tattccaccg ccgccagtcg ctatagccca acgtttgtta ccgaatcggg
    81361 cgagatacca tcaattacag cgttatcagc acaaatggaa aagctggtat cagttgaaat
    81421 tacaacaaga aggcgtgatg ccattacatc atcgagagga acactatgtt gaataaaacc
    81481 gtggaggtca gaaacagtac tggcctgcat gcacgtccgg cggcctgtct ggcgaaagcc
    81541 gccaaaaaat atagctgtaa agtgacgctg cactatgagg gtaatgatat taatgccacc
    81601 agcatgatga atattatgcg cgccggaata aaaggcggaa aaacggtgga aatacgctgc
    81661 gacggtgatg atgaaaacga ggccatacag actctcaccg acttatttcg cgaccgcttc
    81721 ggtgaagctg aataattaac tgatattaaa agatgaggtt ttcaatatgc cattagttaa
    81781 tggacggata ctgctcgacc gcattcagga aaaacgggta ttagcgggcg catttaatac
    81841 caccaatctg gagacgacaa tttcaatttt aaacgctatt gagcgttcgg gattacccaa
    81901 cttcattcag attgcgccaa ccaatgcaca actctcggga tacgattaca tttatgaaat
    81961 cgtgaaacgt cacgccgata aaatggatgt tccggtcagt cttcatctgg atcacgggaa
    82021 aacccaggag gatgttaaac aggcggtgcg cgcgggcttt acctcggtca tgattgacgg
    82081 tgccgcattc tcttttgaag agaatatcgc tttcacccag gaagctgtcg atttctgtaa
    82141 atcctatggc gtaccggttg aagcggagct gggggcgatt ctcggcaaag aagatgatca
    82201 cgtcagcgaa gccgattgca aaacggaacc ggaaaaggtg aagacctttg tcgagcgtac
    82261 cggttgcgac atgctggcag tttctattgg caacgttcat ggcctggacg atataccgcg
    82321 tatcgatatc cccctgctga agcgcattgc cgaagtctgt ccggtgccgt tggttattca
    82381 cggcggctcc ggtatcgccc cggagatatt acgcagcttt gttaattatc gcgtcgctaa
    82441 agtaaatatc gccagcgatc tgcgcaaagc gtttatcacc gccgtcggca aagcgtatgt
    82501 gaataatcac aacgaggcga atctggcacg agtcatggca tcagcaaaaa acgccgtcga
    82561 ggaagatgtg tatagcaaaa tcctgatgat gaatgagggg catcggttgg ttagaaaggc
    82621 atcttaagag ccacaaaaat atagcggcgc aacagaaatt gttgggccgc tataagttat
    82681 aaatgattca aacgctcatt cagtgtttca ataatatagg tattaacaga gacttgttgc
    82741 tgcgcagccg cattattaag gcgttcactc agcgattctg gatagcgtaa cgtaaaggtc
    82801 ttaatctttt ctgtacgcgc atacggctca atacccgcag ctttacaatc ctcaagatac
    82861 tctcgcaggg aaagttcccc ttctttttgc agcccctgaa tgctgtcaga gacaaaatca
    82921 caataacctg acaagcccag gaatttgcca cgaaatgcat tcagttctgg cacgtaagtg
    82981 ataacagcag gttgcccggc aatctccata gagtttggcg tcttcagttt gatcatggtt
    83041 ttacccctat acttagtaac cagtcacgca cgctttctac agcgccttta tcggtatctg
    83101 gcgacggatg aggccgatgg aaacacgcaa cgctcatatt tagtatgaac ttacaacgcg
    83161 aacctcttcc ttccttaatt tctccgccta atgctttaac cagtgactct atatcagacc
    83221 acttgatccc ttgaggaaca ggagttttaa atatttgatc caatgtattt ttttgtttct
    83281 tccgtaaagc ctgtaccttg gttctcatgg catgtcctta tgacttcact ttatgacatc
    83341 aatagtatga catcacagta tgaagtcaag tttcccttcc ttacgccatt gtcccaatcg
    83401 tcttcctgaa tcgcagcagc gcaatggtga aaaacgcgcc gccaattgcc atcagcgtca
    83461 ggaactgcgg ccagacgatt tcgaatccgg caccacggta gagaatggcc tgcgcgaggc
    83521 taacaaagtg tgtcgtcggc atgatcagca taatgtcctg caccatctgc ggcatacttt
    83581 cgcgcggcgt ggaaccaccg gaaagcattt gcagcggcag cagcaccaga atcaccagca
    83641 gccccagttg cggcattgaa cgcgctattg tccccataaa aatgccgatt gacgtggtgg
    83701 caaacagact aagcgccacg cccagcataa acagcgggat cgagccttca atcggtacgc
    83761 ccaacacgcc ttttaccatc agcaccagtg ataatcccga taccaccagc accaccaacc
    83821 ccatcgacca gatcttcgcc atcatgatct caaacggcgt tatcggcatc accagtaagt
    83881 gttccaccgt gccgtgttca cgctcgcgga tcagcgccga tccggtcaat acaatcgcca
    83941 gcatggtaat gttgttgatg atcgccatca ccccgccaaa ccacgcggga tcaaggttcg
    84001 ggttaaagcg catccgggtt tccagcgata ccaacggttc gctgttatca cggtagcgcg
    84061 cgacaaagct gttcacttca ccgttgataa tattctggat atacccattg ccggtaaatg
    84121 cctggctcat gcgcgtggca tcgacgttca cctgaatatc tggctggcgt ccggcgagga
    84181 catcacgctg aaaattaggc ggaatattta tcgcaaaggt ataacgcccg gcgtccagtc
    84241 cggcatccat ctcatcggcg gtgatcatct ccggtggcaa aaaccacggg cgatagaagc
    84301 tgttaacgat ccggttcgat aactgcgatt gatccatatc ggcaatggcg atcggtgcca
    84361 ggttcaacga tcctggcgtg acggtcgctg acgaatacac cgacaccgta aacgagaaga
    84421 caatcaatgt cagcatcgct ttatcaccga gtagactgcg caactctttg atacccagat
    84481 taaaaatatt gcgtaaatgg cgcatcatcc ctcctgtttt ttcagcagca ggatgcttaa
    84541 gcccatcacc agcggtatgg ctatcagcaa cgggataaaa agttgccaca aatcagtcag
    84601 atccagcgct ttcgagaacg tcccgcgggc gatagtcaga aaatgactgg tcgggtaaac
    84661 ctcgccgatc caacgtcccg gcccttccag cgaagctacc ggatcgatca tcccggaaaa
    84721 ctgtgtcgcc gggatcaacg tgataatcgc cgtaccgaaa atggcggcaa tctggctttt
    84781 cataaaggtg gagatcagca gccccattcc ggtggcgatg atgatataca gcagcgccgc
    84841 cagggtgagc gtcaggaaac tgcctttatg cggtacgcca aacacaaaca ccgacaggcc
    84901 gcagagcagg aaaaagttca gcatccccag cgcgatgtac ggcagctgtt tgccaagcaa
    84961 aaattcgcta cgggtggtgg gggtcacgta aaggttgata atcgacccta gctctttttc
    85021 ccgcaccacg ctaagggcgc ttagcattga cgggatcatc atcagcagaa gcgggatcac
    85081 cgccggaaca atcgctggca ggctttttac gtccgggtta tagcgataac gcgtctcaat
    85141 attcatcagc ccgctttggc tggagggtgt ggattgtcgg ctcgccacat cctgtagcca
    85201 gctctggtgc atggcctgca cgtaaccttt tacagtttcg gcgcggctcg gcatcgcacc
    85261 gtcgatccag acaccgagtt ccacaggcgt accacgcgcg atatcgcgcc cgaaattggg
    85321 cgggatctca attgccaccg tgatatcgcc cgcacgcatc cgacgatcaa gctcgtcata
    85381 actggtaagc agcggctgtt cgataaagta acgggaaccg gagagattga gcgtccacgc
    85441 ctggctactg acggtctggt cgcggtcaag caccgcaaag cgcaggtttt ccacatccat
    85501 actgatgccg taacccatta tcagcatcag gatcactgtt cccatcagcg ccagcgtcga
    85561 acgtaccgga tcgcgtcgca gttccagcgc ttcgcggcgg ctgtagctaa acagacggcg
    85621 caggctaaat ccctggcgcg gcgcgtgtgt ggtgtcgtgt atcacgggag gcgcttcggc
    85681 ttcgttgctc tgtcctgccg cttcctgcaa ataggcgata aatgcctctt ccagactggc
    85741 ggctccgcgt ttctcaacca gttcctgcgg tgtaccgctg gcgagcactt ttccggcgtg
    85801 catcagagag atacggtcgc agcgttccgc ttcgttcata aagtgggtgg agatgaagat
    85861 agtcactttg tcctggcgcg agagatcgac catcaactgc cagaacatat ccctcgccac
    85921 cggatcgacg ccagaagtag gttcatcgag gattaacatt tccgggcgat gaatcaccgc
    85981 caccgccagc gaaagccgct ggcgaatgcc gagcggcaat gactccggca gaacatcttc
    86041 aacgtcgttg agcttaaaac gctcgctcat ttcagccact cttgcgggaa tttccgcttc
    86101 cgggatgtga aacaaacggg catgtaactc aaggttttgc cgcacggtga gctcgttata
    86161 gagcgaaaac gcctgcgaca tatagcccac ccgacggcga gtatcgatat cttttggatc
    86221 aaccggttgc ccgaacagcc acgcctcacc ttcgctggcg ggcagcaatc cggtaagcat
    86281 tttcatggtg gtggatttac cgcagccgtt cgaaccaaga aaaccaaaaa tctccccgcg
    86341 tggaatgcgg aaattgacgt gatcaacggc aacgaaggaa ccaaaacgca tggtcagatc
    86401 gcgcgcttcg atggcaatct ctgcgttttc aggttgatat ggtgggatca ctaccgcctg
    86461 atgcgcctgg cgttgcgctt gcggtaacag atttataaat gcttcttcca gcgtggcgct
    86521 ttgcgtttgc tgccgcagct cttcggcgct gccagttgcc aacacttctc cggcattcat
    86581 cgccaccaac cagtcgaagc gttccgcctc ttccatatag gccgtggcga ccagaacgct
    86641 catattgctc tgccgctggc gaatactgtc gatcagatcc cagaactggg cgcgggagag
    86701 cgggtcaacc cccgtcgtag gctcatcaag aatcaacagt tccgggtcgt ggattaacgc
    86761 acagcacagc cccagttttt gcttcatccc gccggagagt ttccctgccg gacgatcgcg
    86821 aaacggtgct aacccggtgc tggtcagcag ctcattgatt cgcacttccc gctccgcttt
    86881 gtcgtgaccg aacaggcgag cgaaaaaatc gacgttttca tacaccgaca aggtgtggta
    86941 gaggtttttg cccagcccct gcggcatcca ggcgatgcgc gggcagacgt cgcggcgatg
    87001 cttcgggtcg cgcatatcgc cgcccagcac gatcacattg ccctgctcaa tgacacgagc
    87061 accggaaatc aatgacaata aactcgactt cccgacgcca tccggaccaa tcagcccgac
    87121 catacagcgg gccggaatat cgagtgtgat attgttcagc gcaacggttt ttccataatg
    87181 ctggctcacg cccgccagtt gcgcgacagg cgggacggga accagttcca gatgcgtcat
    87241 tgcggcaacc tcaccacgag gtcgtcaggc cacggaagtt cttcattcac ccgcacccac
    87301 gctacgcccg gcaagcccgt tttgacatat tccagatgct gctggagtaa ttccggtggg
    87361 atacgcgctt tgacgcggaa catcagtttc agccgttcat cgctggtttc gacggttttt
    87421 ggcgtgaact gggcgacact ggcgacaaaa ctgatggttg caggaatacg cagatctggc
    87481 gcggcatcga ggatcagccg ggcttcaccg ccaagtttca gcgtgcccgc ctgttcggtt
    87541 ggcaggaaga aggtcatata gacgtcgctg agatcgacca tattcagcac ccgaccgcct
    87601 gccgccagca cttcccctgg ctcggcaacc cgatactgca cgcgtccgtc acgcggggct
    87661 ttcagttcgc tgtcatcgat atctgcggca atgcgccgtt cagtggcttg tgcggcttcg
    87721 acgcgggttt gcgcctgaat gatattggtg cgtgccgctt ctatagcggc tttagaagcc
    87781 gaaacctggg cttttgctga ttccagcgca gctcgggcgc tctcagcggc ggcgcgatcg
    87841 tcatccagct gttgcgcaga aatagcccct cgttgggcga gcgagcggga acgcgtatga
    87901 cgtttggcaa cggagtccag ttcggcctgg cgttgattaa ccagcgactg tgcggcgcga
    87961 gtttcgcttt gtcgttgctc cagcaaagcc tgcgcggcgg caacggcgct ttgtgcctct
    88021 ttgatttgcg cgatggcttc cagtcgctgc tcctgcaaca cgcgagtatc catcttcgcc
    88081 agcacttcgc cttcgcgaac aaactggcct tctttcacca gaatggtgtc gatacgcccg
    88141 gcaattttgc tggcgatatc cacttccgtc gcttcaatgc gcccattgct gacggcaaag
    88201 ccttccggca cacctgccgg gcgcaacagc caccacgcta cgatagcagc caccgccagt
    88261 aacccgacac cccaccacgc cagatggcgc ttactcttat ccataatcga cccgccataa
    88321 tccctgtaag caaacgacgc gtgttacaac tgccgccgaa cgacgttcag cggattcttc
    88381 catccgggat aagaaggaaa cgacaaatac gaccagcgcg cagagcattg agcgccaacg
    88441 gggaaggctt agtgaaaacg ggtagtaatg ttgtcatcat cagcattcct ggccgtaaat
    88501 gaaacacgca tactcataag tgtgatctat aaaactggct gacaaaacgg tatccatcct
    88561 gttggatagc gtttcacgcc tgttcagttg caggaacaga gcattcctta ttacgtttta
    88621 tcaacagtct caaccagata gtaacattaa tattcatgtt gtctatggtt cagagctgta
    88681 acattgtgcg ccttctaatc aaaggcataa gtccatttct gtttttttca tgttggtaag
    88741 acttccaggg agtggttatc tcaggccccg cactagggtt aatttggcaa ttatgcatgc
    88801 tgaacattct tcccgtatgt ttactgtcac gggcgttaag acctaaaact gtccccgttt
    88861 cacctatgca ttctcttcca ccattaccgt tatgtgcaac aacttgattt atacctaaca
    88921 tttgtgagca tgctaatagt gcttcaagtt taggtctgta atctgtcgaa acagaaaaat
    88981 aacgattaaa ggatcgacaa gagatagccc ggctagtttt aaacttttta ctattaatta
    89041 tagtatgatg ctttttatta ataattgccg ctaattcgac cggatcgaga gcattggtca
    89101 tttcatcaat agagaccgtt atcggtccta taatgtaacg ttttcgctga tcatcaaaag
    89161 ttatcccatg atgatttgcc attattttgt atttggagtc atagaaacat acaggataat
    89221 cttttatccc gtcgtaggta tcctcatcaa gtggtttcat acgcgtgaag ttctgcatat
    89281 agttaccatt gcaattcgtt tcatgatttc cagctaatac atgaacgtta tcattgatgc
    89341 tcattatgcg ttttaacaaa tcgatgataa attgatcgcc acagatatta ctaaatcgat
    89401 cgccggtttg gtcacccaaa aacgtacatg gcgtcactgc gtctttatta ataactaaat
    89461 aaggctgaag actttctaaa ataatgtctc ttctgttcag gatactgatt attgtattaa
    89521 cattagtatc aacttgctca ttaaagttct cccttagcaa tacattgaga acttcatctt
    89581 ctatattaag caactggcta aggaaatttt ctccttcagc catgattccc aaatgtcccg
    89641 ttgcaaaaag cgcgtacaaa actgcaccaa ctgagccatc ggtatcacca aaataggttg
    89701 gtctggtact tgtgggatca acaggtgaac aattggagaa atagattctg tcagcgaact
    89761 ggggttttat aattgtttcg aatttaatat cagatacatt gtattgcgat attttccagt
    89821 tccagatggg tttgtacgac tcagtaactg caacatcatt agcattatca tttactgtca
    89881 tattattaag taaataaaca atgccgcacc aattaatatc ggtattatca agagggcaac
    89941 cgtaattttt gctattaatc gtcacaataa actgataatg gttattccta cgtagaatat
    90001 gagcacgcat gcccgatagc atactatatt tttctgaata atccgtttgt atggtaaatg
    90061 aaattttgtc atcttgaata gattgttgga accgttctat ttgccaatca tctacttttt
    90121 ccagaacctc accgagcatt gtcctggcaa tacttgatat atcatcacct ataccatttg
    90181 caatataccc aaacagttct ttagtgaagt tagtaacatg ctcttgcacc atatcatttg
    90241 ttgcgattgt cgttgtactg tcgttagtac ctgccacagt tccaatttt
//
LOCUS       DAZFBR010000006        86484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFBR010000006 DAZFBR010000000
VERSION     DAZFBR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.006"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(557..1228)
                     /gene="queE"
                     /locus_tag="ACHJZ2_000518"
     CDS             complement(557..1228)
                     /gene="queE"
                     /locus_tag="ACHJZ2_000518"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO4566691.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1383..2003
                     /locus_tag="ACHJZ2_000519"
     CDS             1383..2003
                     /locus_tag="ACHJZ2_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO4566692.1"
                     /translation="MKRDLNTLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLN
                     QYQRRADLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQ
                     QAQAQVTRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARERYIKAIEQYN
                     VTIRKFPAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            2017..2691
                     /locus_tag="ACHJZ2_000520"
     CDS             2017..2691
                     /locus_tag="ACHJZ2_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4566693.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            2925..4073
                     /locus_tag="ACHJZ2_000521"
     CDS             2925..4073
                     /locus_tag="ACHJZ2_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4566694.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWNPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            4070..4984
                     /locus_tag="ACHJZ2_000522"
     CDS             4070..4984
                     /locus_tag="ACHJZ2_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4566695.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(5044..6342)
                     /gene="eno"
                     /locus_tag="ACHJZ2_000523"
     CDS             complement(5044..6342)
                     /gene="eno"
                     /locus_tag="ACHJZ2_000523"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO4566696.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(6430..8067)
                     /gene="pyrG"
                     /locus_tag="ACHJZ2_000524"
     CDS             complement(6430..8067)
                     /gene="pyrG"
                     /locus_tag="ACHJZ2_000524"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO4566697.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(8295..9086)
                     /gene="mazG"
                     /locus_tag="ACHJZ2_000525"
     CDS             complement(8295..9086)
                     /gene="mazG"
                     /locus_tag="ACHJZ2_000525"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO4566698.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(9157..9492)
                     /gene="mazF"
                     /locus_tag="ACHJZ2_000526"
     CDS             complement(9157..9492)
                     /gene="mazF"
                     /locus_tag="ACHJZ2_000526"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO4566699.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(9492..9740)
                     /gene="mazE"
                     /locus_tag="ACHJZ2_000527"
     CDS             complement(9492..9740)
                     /gene="mazE"
                     /locus_tag="ACHJZ2_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO4566700.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(9818..12052)
                     /gene="relA"
                     /locus_tag="ACHJZ2_000528"
     CDS             complement(9818..12052)
                     /gene="relA"
                     /locus_tag="ACHJZ2_000528"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO4566701.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(12100..13401)
                     /gene="rlmD"
                     /locus_tag="ACHJZ2_000529"
     CDS             complement(12100..13401)
                     /gene="rlmD"
                     /locus_tag="ACHJZ2_000529"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO4566702.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            13458..16214
                     /gene="barA"
                     /locus_tag="ACHJZ2_000530"
     CDS             13458..16214
                     /gene="barA"
                     /locus_tag="ACHJZ2_000530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO4566703.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(16447..17787)
                     /gene="gudD"
                     /locus_tag="ACHJZ2_000531"
     CDS             complement(16447..17787)
                     /gene="gudD"
                     /locus_tag="ACHJZ2_000531"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO4566704.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(17808..19148)
                     /gene="gudX"
                     /locus_tag="ACHJZ2_000532"
                     /pseudo
     CDS             complement(17808..19148)
                     /gene="gudX"
                     /locus_tag="ACHJZ2_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(19150..20502)
                     /gene="gudP"
                     /locus_tag="ACHJZ2_000533"
     CDS             complement(19150..20502)
                     /gene="gudP"
                     /locus_tag="ACHJZ2_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO4566705.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(20937..21386)
                     /gene="yqcA"
                     /locus_tag="ACHJZ2_000534"
     CDS             complement(20937..21386)
                     /gene="yqcA"
                     /locus_tag="ACHJZ2_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO4566706.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(21404..22186)
                     /gene="truC"
                     /locus_tag="ACHJZ2_000535"
     CDS             complement(21404..22186)
                     /gene="truC"
                     /locus_tag="ACHJZ2_000535"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO4566707.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSGEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(22186..22515)
                     /gene="yqcC"
                     /locus_tag="ACHJZ2_000536"
     CDS             complement(22186..22515)
                     /gene="yqcC"
                     /locus_tag="ACHJZ2_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO4566708.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(23137..23682)
                     /gene="syd"
                     /locus_tag="ACHJZ2_000537"
     CDS             complement(23137..23682)
                     /gene="syd"
                     /locus_tag="ACHJZ2_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO4566709.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            23750..24598
                     /gene="queF"
                     /locus_tag="ACHJZ2_000538"
     CDS             23750..24598
                     /gene="queF"
                     /locus_tag="ACHJZ2_000538"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO4566710.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            24710..26074
                     /gene="ppnN"
                     /locus_tag="ACHJZ2_000539"
     CDS             24710..26074
                     /gene="ppnN"
                     /locus_tag="ACHJZ2_000539"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO4566711.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            26631..27920
                     /gene="sdaC"
                     /locus_tag="ACHJZ2_000540"
     CDS             26631..27920
                     /gene="sdaC"
                     /locus_tag="ACHJZ2_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO4566712.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            27978..29345
                     /gene="sdaB"
                     /locus_tag="ACHJZ2_000541"
     CDS             27978..29345
                     /gene="sdaB"
                     /locus_tag="ACHJZ2_000541"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO4566713.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            29457..30212
                     /gene="xni"
                     /locus_tag="ACHJZ2_000542"
     CDS             29457..30212
                     /gene="xni"
                     /locus_tag="ACHJZ2_000542"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO4566714.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(30267..31415)
                     /gene="fucO"
                     /locus_tag="ACHJZ2_000543"
     CDS             complement(30267..31415)
                     /gene="fucO"
                     /locus_tag="ACHJZ2_000543"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO4566715.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(31443..32090)
                     /gene="fucA"
                     /locus_tag="ACHJZ2_000544"
     CDS             complement(31443..32090)
                     /gene="fucA"
                     /locus_tag="ACHJZ2_000544"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO4566716.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            32637..33953
                     /gene="fucP"
                     /locus_tag="ACHJZ2_000545"
     CDS             32637..33953
                     /gene="fucP"
                     /locus_tag="ACHJZ2_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO4566717.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGSMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            33986..35761
                     /gene="fucI"
                     /locus_tag="ACHJZ2_000546"
     CDS             33986..35761
                     /gene="fucI"
                     /locus_tag="ACHJZ2_000546"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO4566718.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRDPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            35870..37288
                     /gene="fucK"
                     /locus_tag="ACHJZ2_000547"
     CDS             35870..37288
                     /gene="fucK"
                     /locus_tag="ACHJZ2_000547"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO4566719.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            37290..37712
                     /gene="fucU"
                     /locus_tag="ACHJZ2_000548"
                     /pseudo
     CDS             37290..37712
                     /gene="fucU"
                     /locus_tag="ACHJZ2_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
     gene            37770..38501
                     /gene="fucR"
                     /locus_tag="ACHJZ2_000549"
     CDS             37770..38501
                     /gene="fucR"
                     /locus_tag="ACHJZ2_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO4566720.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(38545..39645)
                     /gene="rlmM"
                     /locus_tag="ACHJZ2_000550"
     CDS             complement(38545..39645)
                     /gene="rlmM"
                     /locus_tag="ACHJZ2_000550"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO4566721.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(39638..40033)
                     /gene="ygdD"
                     /locus_tag="ACHJZ2_000551"
     CDS             complement(39638..40033)
                     /gene="ygdD"
                     /locus_tag="ACHJZ2_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO4566722.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(40052..40969)
                     /gene="gcvA"
                     /locus_tag="ACHJZ2_000552"
     CDS             complement(40052..40969)
                     /gene="gcvA"
                     /locus_tag="ACHJZ2_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO4566723.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(41320..41547)
                     /gene="ygdI"
                     /locus_tag="ACHJZ2_000553"
     CDS             complement(41320..41547)
                     /gene="ygdI"
                     /locus_tag="ACHJZ2_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO4566724.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            41739..42944
                     /gene="csdA"
                     /locus_tag="ACHJZ2_000554"
     CDS             41739..42944
                     /gene="csdA"
                     /locus_tag="ACHJZ2_000554"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO4566725.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            42944..43387
                     /gene="csdE"
                     /locus_tag="ACHJZ2_000555"
     CDS             42944..43387
                     /gene="csdE"
                     /locus_tag="ACHJZ2_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO4566726.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(43438..44244)
                     /gene="tcdA"
                     /locus_tag="ACHJZ2_000556"
     CDS             complement(43438..44244)
                     /gene="tcdA"
                     /locus_tag="ACHJZ2_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO4566727.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINSECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(44483..45580)
                     /gene="mltA"
                     /locus_tag="ACHJZ2_000557"
     CDS             complement(44483..45580)
                     /gene="mltA"
                     /locus_tag="ACHJZ2_000557"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO4566728.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            45789..45865
                     /locus_tag="ACHJZ2_000558"
     tRNA            45789..45865
                     /locus_tag="ACHJZ2_000558"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45823..45825,aa:Met,seq:cat)
     gene            complement(45939..47192)
                     /gene="amiC"
                     /locus_tag="ACHJZ2_000559"
     CDS             complement(45939..47192)
                     /gene="amiC"
                     /locus_tag="ACHJZ2_000559"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO4566729.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            47424..48755
                     /gene="argA"
                     /locus_tag="ACHJZ2_000560"
     CDS             47424..48755
                     /gene="argA"
                     /locus_tag="ACHJZ2_000560"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO4566730.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(48817..50643)
                     /gene="recD"
                     /locus_tag="ACHJZ2_000561"
     CDS             complement(48817..50643)
                     /gene="recD"
                     /locus_tag="ACHJZ2_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO4566731.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(50643..54185)
                     /gene="recB"
                     /locus_tag="ACHJZ2_000562"
     CDS             complement(50643..54185)
                     /gene="recB"
                     /locus_tag="ACHJZ2_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO4566732.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(54178..57066)
                     /gene="ptrA"
                     /locus_tag="ACHJZ2_000563"
     CDS             complement(54178..57066)
                     /gene="ptrA"
                     /locus_tag="ACHJZ2_000563"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO4566733.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(57242..60610)
                     /gene="recC"
                     /locus_tag="ACHJZ2_000564"
     CDS             complement(57242..60610)
                     /gene="recC"
                     /locus_tag="ACHJZ2_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO4566734.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(60623..60946)
                     /gene="ppdC"
                     /locus_tag="ACHJZ2_000565"
     CDS             complement(60623..60946)
                     /gene="ppdC"
                     /locus_tag="ACHJZ2_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO4566735.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(60931..61338)
                     /gene="ygdB"
                     /locus_tag="ACHJZ2_000566"
     CDS             complement(60931..61338)
                     /gene="ygdB"
                     /locus_tag="ACHJZ2_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO4566736.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(61335..61898)
                     /gene="ppdB"
                     /locus_tag="ACHJZ2_000567"
     CDS             complement(61335..61898)
                     /gene="ppdB"
                     /locus_tag="ACHJZ2_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4566737.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(61889..62359)
                     /gene="ppdA"
                     /locus_tag="ACHJZ2_000568"
     CDS             complement(61889..62359)
                     /gene="ppdA"
                     /locus_tag="ACHJZ2_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4566738.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(62543..63337)
                     /gene="thyA"
                     /locus_tag="ACHJZ2_000569"
     CDS             complement(62543..63337)
                     /gene="thyA"
                     /locus_tag="ACHJZ2_000569"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO4566739.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(63344..64219)
                     /gene="lgt"
                     /locus_tag="ACHJZ2_000570"
     CDS             complement(63344..64219)
                     /gene="lgt"
                     /locus_tag="ACHJZ2_000570"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO4566740.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(64370..66616)
                     /gene="ptsP"
                     /locus_tag="ACHJZ2_000571"
     CDS             complement(64370..66616)
                     /gene="ptsP"
                     /locus_tag="ACHJZ2_000571"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4566741.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(66629..67159)
                     /gene="rppH"
                     /locus_tag="ACHJZ2_000572"
     CDS             complement(66629..67159)
                     /gene="rppH"
                     /locus_tag="ACHJZ2_000572"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO4566742.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(67512..67658)
                     /gene="ygdT"
                     /locus_tag="ACHJZ2_000573"
     CDS             complement(67512..67658)
                     /gene="ygdT"
                     /locus_tag="ACHJZ2_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO4566743.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            67844..68533
                     /gene="mutH"
                     /locus_tag="ACHJZ2_000574"
     CDS             67844..68533
                     /gene="mutH"
                     /locus_tag="ACHJZ2_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO4566744.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            68602..69315
                     /gene="ygdQ"
                     /locus_tag="ACHJZ2_000575"
     CDS             68602..69315
                     /gene="ygdQ"
                     /locus_tag="ACHJZ2_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4566745.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            69453..69671
                     /gene="ygdR"
                     /locus_tag="ACHJZ2_000576"
     CDS             69453..69671
                     /gene="ygdR"
                     /locus_tag="ACHJZ2_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO4566746.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            69779..70819
                     /gene="tas"
                     /locus_tag="ACHJZ2_000577"
     CDS             69779..70819
                     /gene="tas"
                     /locus_tag="ACHJZ2_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO4566747.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(70851..72044)
                     /gene="lplT"
                     /locus_tag="ACHJZ2_000578"
     CDS             complement(70851..72044)
                     /gene="lplT"
                     /locus_tag="ACHJZ2_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO4566748.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(72037..74196)
                     /gene="aas"
                     /locus_tag="ACHJZ2_000579"
     CDS             complement(72037..74196)
                     /gene="aas"
                     /locus_tag="ACHJZ2_000579"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO4566749.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            74782..75813
                     /gene="galR"
                     /locus_tag="ACHJZ2_000580"
     CDS             74782..75813
                     /gene="galR"
                     /locus_tag="ACHJZ2_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO4566750.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(75820..77082)
                     /gene="lysA"
                     /locus_tag="ACHJZ2_000581"
     CDS             complement(75820..77082)
                     /gene="lysA"
                     /locus_tag="ACHJZ2_000581"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO4566751.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMFDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            77204..78139
                     /gene="lysR"
                     /locus_tag="ACHJZ2_000582"
     CDS             77204..78139
                     /gene="lysR"
                     /locus_tag="ACHJZ2_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO4566752.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(78126..78818)
                     /gene="ygeA"
                     /locus_tag="ACHJZ2_000583"
     CDS             complement(78126..78818)
                     /gene="ygeA"
                     /locus_tag="ACHJZ2_000583"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO4566753.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFKLS"
     gene            complement(78947..80365)
                     /gene="araE"
                     /locus_tag="ACHJZ2_000584"
     CDS             complement(78947..80365)
                     /gene="araE"
                     /locus_tag="ACHJZ2_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO4566754.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(80680..81441)
                     /gene="kduD"
                     /locus_tag="ACHJZ2_000585"
     CDS             complement(80680..81441)
                     /gene="kduD"
                     /locus_tag="ACHJZ2_000585"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO4566755.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(81471..82307)
                     /gene="kduI"
                     /locus_tag="ACHJZ2_000586"
     CDS             complement(81471..82307)
                     /gene="kduI"
                     /locus_tag="ACHJZ2_000586"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO4566756.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(82594..83775)
                     /gene="yqeF"
                     /locus_tag="ACHJZ2_000587"
     CDS             complement(82594..83775)
                     /gene="yqeF"
                     /locus_tag="ACHJZ2_000587"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO4566757.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            84030..85259
                     /gene="yqeG"
                     /locus_tag="ACHJZ2_000588"
     CDS             84030..85259
                     /gene="yqeG"
                     /locus_tag="ACHJZ2_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4566758.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            85719..86351
                     /gene="yqeH"
                     /locus_tag="ACHJZ2_000589"
     CDS             85719..86351
                     /gene="yqeH"
                     /locus_tag="ACHJZ2_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4566759.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWTEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT        21454 a        22949 c        20895 g        21186 t
ORIGIN      
        1 tgtatcgata attgtgaact tttcatatat ggagagctat tttgaacatg aggggttacg
       61 tggatatgtt gcttattacg agtactgcta atataaaaac ttgagaaaga gataacgggt
      121 tatatggtgg tttatcccca ctggcgcggg gaactcctcg actttaattt tttcgctgcc
      181 cgcttttgcg gtttatcccc gctggcgcgg ggaactctct aaacataacc tattattaat
      241 taatgatttt ttaagccagt cacaatctac caactttaca gtatcacaca aacaacacat
      301 ccattatgtt aaagagcact taacccattg attaaaaagg taaatattta aaataactct
      361 atacaaacta aaatctacca aactttaccg caataatttt cactccagcg aaaaattaac
      421 gccacagaat ttgtagagaa tgataatgat tagtccggga taggcaagat ggatgagttt
      481 tcagagacga caaagaatat aaatgagtag aagtctttac aggttccacc catttataac
      541 gcttataaat gtttaatcag gcaatattta aatatttatg ggtttgcatc gacaaacgcc
      601 aattacgcgc aatgcaggtt tcaatgcaca aacgtgtggc atcatccttt tggctaatcg
      661 gctgcagtgc aatgactcgc ggtttatcat cggtcagcgt cgccaaaagt tcatccagtg
      721 cttcaatatc acgtacgcgt cccactggat gcttgatttc gttggctcgc tccagtgcct
      781 gtgacaacac ttcatagccg ccgcgcatgt tcagctttgg cgatacggta acccaggtat
      841 tcggtgtaca gcgtacttca tgagtaccac tggtttcgat ctggcagcta aaaccgttct
      901 tttcgagcag gtcagtcagt ggcagcaaat catgaatgca aggctcacca cccgtaatca
      961 ccacatgccg cgcggtatat ccctggcgac caatgacagc cagcaagtct tcactgctcg
     1021 ccgcccccca cttatcactc tctttggtct tcgccagaat gctgaaaagg gagacttccc
     1081 gatcctcaag cttttcccag gtgtgtttgg tgtcgcacca ggcacagcca accgggcatc
     1141 cctgtaaacg aataaaaatg gcggggacgc cggtaaagta accctcacct tgcagggtct
     1201 ggaacatctc gttaatcggg tactgcatag cattctctgt gaagtggata attgttaatt
     1261 attgcagatc ctgccacaac aatcatgtct tattaacatt ctgttacagg caggtttatg
     1321 gcaaaaagtg gattagatca taaagaataa aatgaagcag agtattatgt tttaaggaat
     1381 taattaaaag ggatttaaac acgctcagaa tattcatcgt aattatattt attttcaatt
     1441 tatcaggctg tggatataac gatattcaaa cctatgatga gcaggttaac gcctcctggt
     1501 ctgaggtatt gaatcaatat caacgtcgcg ccgatcttat tcctaatctt gtcgccagca
     1561 ttaaaggtta ttccagccat gaacgagatg tactggaagc cgtaacattg gctcgtagcc
     1621 aggctaatcg cgccagtagc gatcttcagc aaactccggg agatgaacaa aaattacaag
     1681 cctggcaaca ggctcaagcg caggtgactc gcactctcgg tcagttaacg ataattagtg
     1741 agcgttaccc tgagttaaaa tctcaggaac tctatcaaaa tttaatggta cagcttgaag
     1801 gaagtgaaaa tcggattgcc gttgcgcgag aaagatatat taaagccatt gaacaatata
     1861 atgtcactat ccgcaaattc cccgctgtat taaccgcaaa ggtcatggat tacacaccaa
     1921 agaaaaatta tttgccggat gatgtcgccg cagtaagtaa agccccaaca atagatttta
     1981 gccagaatgc taatgctcat taagcgggga agttctatgc gaaattttat cttccttatg
     2041 gctttcttct gttcatctgt gtttgccaca caaattccag tacctgaatc acccaagtat
     2101 gtgaatgact taaccggcac attaacaaac agcgaagtaa acaccctgac caaccagatt
     2161 aaagccctga cgcaaaaaaa ctacgcacag ttggtcgtgt tagtggttga aacaacaggc
     2221 gatgaaacca tcgagcagta cgcgacgcgg gtattcgata gctggaaacc gggtgataaa
     2281 gaccgcgatg atggcgttct actgttagtc gcctggcagg atcataccgt gcgtattgaa
     2341 atcggttacg gactggaagg tattattacg gatgcgcagt ccgggaaaat tattcgcaac
     2401 agtattattc ctgcatttaa aaaaggcgac ctggctggtg gtttgcaaaa gggaatcagc
     2461 gatattgaaa gtcgtctgac ggggaataat ttagcaacga taacaccgac cgatcaccct
     2521 ttgcccttta gcggttggtg ggcattgtta gtctgggcga tagtactgac gtttatttct
     2581 gccagaggat atatcaaaac gctgggagta atttgcttcg cagctattgt actggccttt
     2641 gttttaccta tagcaggctt cagcggaagc tggggagtac tcgcaacatg actctgcttc
     2701 gccacaccgt tcctggctgt cgccatcgct tttaccccct tcggcaaaaa agttcgggat
     2761 ttgatgcgtg atgccaacca gccatcacgc catacaagaa gcaattcttc ctcccactat
     2821 tcatcatcgt catcctcttc attcgacaat gacaatttta gcggtggtgg cggttcatct
     2881 ggtggcggtg gcgcttccgg gcgctggtaa caaaaggaat tgatatgcgt atttttctct
     2941 tacttctgat gctatgcggt tttcaggtat tcgctgaacg attgccttca gtgactgact
     3001 taaccgggac attaacgaca gaagaacaaa ctgctttaac tcaacaatta cagactctcg
     3061 agcagcaaaa ccacttccag gtggccgtgt tggttacgcc gaccacaggg ggcaaaaaca
     3121 ttaaacttgc agcctcgcag agatacgggg tatatcactg gaaccccgtt ggagagaagc
     3181 gatatggcga aggtatctta attctcgtcg tatggccgga agggctggcg tcaatgaaga
     3241 ttggacatgg actggaagag atgttgccgc cggaacaagc tgcgcaaatt gttcgttacc
     3301 atatgcagcc agaattcgaa aaaaacaatc tctttgctgg attaacgggc ggaattgaaa
     3361 gcatcgcaca gtttacccat atcaaagcga accttagccc tttggatgct ttagccaatc
     3421 atctgtttgc taatccgcaa cgttcgctgc catgtcttgc ctggacactg ctgatgatcg
     3481 ttgccatcgt tgtcctctgg ctatttgcca gccgcccacg gcgtggcata tggatgatat
     3541 cgatgattac ccctatggtc tggtttttct cctttcagga cgatgtcatc attcggcggg
     3601 tatcggtcgt ttgttttagt ttattgttag caacaacctg caagcagcgt ctcgcggtag
     3661 tttttaatat gtgccgcgta tttcctatga tgctgacccc aaaaaataaa aacgctaaag
     3721 tgaaaaaaca aaagaagact ccgcaggttc gtgaatcagg acgaaattca ttgtttgtcg
     3781 tgatgctgat aattattgtg ggatggtgcc tggtgttcgc cagtaataaa gatattgcat
     3841 tcatctcaac aattattggg ttaattgatc attttatcgg cccaataacc attgctggca
     3901 tcgttctgct gatcattgtg gccaaattta ctggcaatct taagctgggg tcaggagaaa
     3961 aaagcaacag gaaaacgggg aatcgtaatg ctcattctcg ctcttcgtcg agaaattctt
     4021 tccgcggtgg tggcggttct tcaggaggcg gcggttcatc cgtccgttgg tgatcgtaat
     4081 gcgcttaatc tttattctat tcactttgtg gtgccttcct gggcttgccc agcaaattgc
     4141 tgtgccggaa ctgcgccaac aggtgaccga tattactggt acgttaagca cttctgaaca
     4201 acaatcactg acacagcaac tgcaggatat tacacataaa acacgggcgc aggtcgctgt
     4261 attagtagtg ccgtccaccg gagacgattc cattgaacaa tatgctacaa gggtttttga
     4321 tagctggaaa ctgggagata agcagagaaa cgatggtatt ttacttctgg ttgcctggga
     4381 ggatcatgct gttcgtattg aggttggcta cgggctggaa ggggtggtta ccgacctgca
     4441 agcggcgaag attatcaggg atatattgat ccctgccttc aaaagtgatg acctgatggg
     4501 tggattaaca ctggcaagcg aaaatatcgg cgcgcttctg ttgaatggtg aattaccgga
     4561 agacaggggg gattattaca gtatcaatcc ccctattcca ttatcgcttg ctgtaattat
     4621 attgctggcg gtactttcat attttattgt ttttaccgat ccgtcgaatt taccctggat
     4681 cactctcact ggcgccattt acgggatggt attcctctat gttgccgagc caggaccatg
     4741 gaccaattta atcgttgcct gcggtatgtt aacgcctttt gcgatcgtac cactggttat
     4801 tttttggctt atcgtcaata aaaagctacg cgccaaatac aaaaaactca gtaaagatag
     4861 agcttcaaga aaaggttctt cttcatcctc ttccggtggc ggctcaagtg gcggaggctc
     4921 aggcggcgga tttagcggcg ggggcggttc ttctggcggt ggcggcgcgt ccggtcgctg
     4981 gtaactcaac cataaaaaac gccagcccga aggctggcat ttttaaatca gataaagtca
     5041 gacttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt cgcccagagc
     5101 ttcttcgata cgaatcagct ggttgtattt agcaacgcgg tcagaacggc tcatagaacc
     5161 agttttgatc tggcctgcag cagtaccaac agccaggtca gcgatggtag cgtcttcagt
     5221 ttcgccagaa cggtgagaga taactgcagt gtagccagcg tctttcgcca tcttgattgc
     5281 agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg agttagcgat
     5341 acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt cgtcaccaac
     5401 cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccatccc agtcagattc
     5461 gtccagaccg tcttcgatgg aaacgatcgg gtactgtttg gtcagttctt ccaggaagtg
     5521 agtgaattct tcagaggtga atgctttgtt gccttcgcca gccagaacgt atttaccgtc
     5581 tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc ccagttcata
     5641 accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg aacccaggtt
     5701 cggcgcatag ccaccttcgt caccaacagc agtgttcatg cctttcgctt tcagaacttt
     5761 tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag ttttcgcgcc
     5821 aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct caccaccgtt
     5881 gatgatgttc atcatcggaa ccggcatgga gtatttgccc ggagtgccgt tcagttcagc
     5941 gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt tagccagaga
     6001 tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt ccaggtcgat
     6061 catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg cctgagcgat
     6121 cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac gggatttgtc
     6181 gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg gagcagctgc
     6241 cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt taccacggga
     6301 gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt cctcaagtca
     6361 ctagttaaac tgaaactcca gacaaacaac gcgtacccag ggtacgcgtt gccgttctaa
     6421 cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca caaagcctgc
     6481 aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact ggcaagccac
     6541 gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc cggaacggcc
     6601 cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga cttcgtaacg
     6661 gtgacgatga cgctcaacaa ttgtcggcgc attgtacagc tggcgaacca ggctatcgtc
     6721 aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc tcttctcgct
     6781 acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca caaccgggta
     6841 cttacagtct ggcacaaatt ccgtagagtt ggcgttctcc atgttggcaa catggcgagc
     6901 gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa tattgttctc
     6961 acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga aaccgccagg
     7021 tacgaggatt gcatccagac ctttaaggat ttcaacgccg cgcgtttcaa catcttgtga
     7081 atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt tcagtgcttc
     7141 aatcacggat ttgtaagcgt ccggcagttc aatgtacttg ccgaccatac caatggtgac
     7201 ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca gatttgcttc
     7261 cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag atttcaacag
     7321 gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt tttccggaac
     7381 attacagaac aatgcaatct tcgcacgttc gttcgccgga acggcgcgat ctgaacgaca
     7441 aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt gctgagtcgg
     7501 tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt gcataaacag
     7561 agtgtgctca cggccaattt caacagccat ctggcgaatc gcttcgagga acggcaggga
     7621 ttcgatatca cctactgtac cgccgatttc gaccagtact acgtcatgac cttcgccacc
     7681 ttccagcacg cgctctttga ttgcgttagt gatgtgcgga ataacctgta cggttgcgcc
     7741 gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac ccgtggtgaa
     7801 gttgttgcgg cggctcattt tggtacggat gaaacgctcg tagtgcccca ggtccaggtc
     7861 ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc tcatagtacc
     7921 tggatcgacg ttgatgtacg gatccagttt catgatggtc acattgaggc cacgggcttc
     7981 aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata cgaccccgcc
     8041 ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg aaaagacgat
     8101 ggaataacca ggacgggaaa gcagtatacc cgaacatgac ctgtgccaca aactttcatt
     8161 atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc ccctttgggg
     8221 taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa atggcgcttg
     8281 accgcgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca gacttcttcc
     8341 attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat acgctccact
     8401 tcgcgaaaac gacgctcgaa tttttcgttc gctttttgca atgcgatttc cgcttttgtc
     8461 cctaaatgac gagccagatt gaccgtggca aacagcaggt cccccatttc ctcctccagt
     8521 ttagcctggt cgacaacagc ctgccgcgct tcgtacatca cctcgtcgat ctcttcgtag
     8581 actttatcga ctaccggacc aagcgtcgtc caatcgaagc caacgttggc gcaacgtttc
     8641 tggatttttt gcgcacgcat taaagccggt aaactacgag gaatatcgtc cagcgccgaa
     8701 tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc aagcacttca
     8761 ctactgtttt cggcagaact gtcagcaaaa acatgcggat ggcgacgctc taatttatcg
     8821 ctaatcgcag cgcaaatatc attaaagtca aagcgccctt cttcctgagc catttgggcg
     8881 taaaacacca cctggaacag cagatcgccg agttcaccgc gcagatcgtc aaaatcttca
     8941 cgggcgatgg cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc aatggtggca
     9001 aatgtctgct ctttatccca cgggcagccg ttttccgggt cgcgcaggcg ctgcataata
     9061 gtgagcaaac ggtcgatttg attcattaga atgtcctgaa aattgcgggt ccgtcaggtg
     9121 gaaacctgtg accagaatag aagtgagtta gtaacactac ccaatcagta cgttaatttt
     9181 ggctttaatg agttgtaatt cctctggggc aaccgttcct ttcttcgttg ctcctcttgc
     9241 ccgccaggcg atacttttta cctgatcagc taacgctacg ccatcacgtt cctgaccgga
     9301 taaaacaact tcgaacggat atccttttga ttgcgttgta caaggaacac acagacacat
     9361 acctgttttg ttgttgtaca tgaacggact caggacaaca gccggacgat gtccggcttg
     9421 ctcgctacct tttgtcgggt caaaatcaac ccaaatcaga tcgcccatat cgggtacgta
     9481 tcggcttacc attaccagac ttccttatct ttcggctctc cccagtcgat attctcgtgg
     9541 aggttttccg gcgtgatgtc gttgaccagt tcagcaagcg taaatacggg ctctttacgc
     9601 actggctcaa taattaattt gccatccacc aggtcaatct tcacttcatc atcaatattc
     9661 agattgagcg cctgcattaa cgtagccggg atccgcaccg ccggtgaatt tccccaacgc
     9721 tttacgctac tgtggatcat aaccctttcc tcaaaccact atcatatgta gatacagtat
     9781 atatcaatct acattgtaga tacgagcaaa tttcggccta actcccgtgc aaccgacgcg
     9841 cgtcgataac atccggcacc tggttgagtt tacccagcac gcgccccagc acttgcaggt
     9901 tgtaaatctc aatggtcatg tcgatggtcg ccagttgctg tttggtgtcg ctacggctgg
     9961 caacgccaag cacgttcacc ttctcgttgg cgagaatggt cgtgatatca cgtaacaacc
    10021 cactacgatc attagccacc acgcggacca ccagcgaata tccggcggag tagctctcac
    10081 cccataccgc gtcaacaatg cgttctggcg catgggagcg cagttccgcc agttgttcgc
    10141 aatcggcgcg gtgtactgaa ataccgcgtc cctgggtaat gaagccgaca atctcatctc
    10201 caggaatcgg ctggcagcag cgcgcgatgt ggtgcatcag gttaccaaca ccttcaacca
    10261 ctacacgacc gttatcttta ctgcggtttt gcggcgtgta gcttttttgc tgaagctgtt
    10321 tcagcgcggc ggcgtcctgc tcttcggcac tcggcttatt aaattgcgat tgcaggaagt
    10381 tcaccatctg attgagacgg atatccccgc caccaatcgc cgccagcaac tcgtcgacat
    10441 cattgaagtt gtaacgcggc agcagatgtt tttctgcttc tttcaggctg atccccagat
    10501 gttccagctc gtcgtcaagg atttgccgcc cagccagaat gtttttgtca cggtcctgtt
    10561 tacggaacca ggcgtgaatt ttcgaacgcc cacggctggt tgtgacgtaa ccgaggtttg
    10621 ggtttaacca gtcacggctg gggttcggct gtttctgggt gataatttca atctggtcgc
    10681 ccatttgcag ctggtaggtg aacggcacaa tgcgcccgcc aatttttgcc ccgatacagc
    10741 ggtgtccgac atcactgtgg atgtggtaag cgaagtccag cggcgttgat cccgcaggca
    10801 aatcaacgac atcaccttta ggcgtaaaga cgtacacccg gtcgtcaaag acctggctgc
    10861 gtacttcgtc gagcatttcg ccggaatcag ccatctcttc ctgccacgca atcagtttac
    10921 gcagccaggc aatccggtct tcatgtcccg aacgtgcgcc gccagcagcc gcgccctctt
    10981 tatatttcca gtgcgcagca acacccaact ctgcatcttc atgcatctgt ttggtgcgga
    11041 tttggatctc aacggttttt ccacccggcc ccagaaccac ggtatgaata gactgataac
    11101 cgtttggttt cgggttagcg acgtaatcgt caaactcatc cggcaggtgg cgatagtgag
    11161 tgtgcactat ccccagtgcg gcataacaat cctgtaaacg ctcggcgaca atacgtaccg
    11221 cacgcacatc aaacagctca tcgaaggcga ggttcttttt ctgcattttg cgccagatgc
    11281 tgtagatgtg tttcggacga ccatacactt cagctttaac gccttcagct ttcatctcag
    11341 cgcgcagatg accgacaaac tcttcgatat agtgttcgcg gtcgagacgg cgttcatgca
    11401 acagttttgc gatgcgtttg tattcggtcg ggtggagata acggaagcag taatcttcca
    11461 gctcccattt cagttgccca atcccaagac ggtttgccaa cggcgcgtag atattggtgc
    11521 actcttttgc cgccagtacg cgttcatctt ccggcgcatc ttttacttca cgcagatgag
    11581 caatacgctc cgccagtttg atgaccacgc agcgaaaatc atcgaccatc gccaataaca
    11641 tccggcgaac gttatcgacc tgttcggagg aaacagaatc agtgtgcgtc gctttcagct
    11701 ggcggatcgc cgccatatca cgcacgccgt gaataaggtt aacgaccgac ttaccgacgc
    11761 tctcacgcag cacatcttcg ctgactacgt tggcatcagc cagagggaac agcagcgccg
    11821 cccgcagcgt gtcaatgtcc atacttaatg tcgagaggat ctccaccatc tcaacaccac
    11881 gccacaataa cagactggca tccggatgcc cctgcgtctg ttgcagacaa tacgcccagg
    11941 tttcggctaa gcactcacac gacttctggc tggtaatacc cagacttgcg atccattttt
    12001 ccggatcaaa ttcaccagcc ttattgatat gtgcacttct taccgcaacc atcgtcctct
    12061 cctttaggga ccagacctgc cgaaatcggc aaatcgcaac tatttaacgc gcgagaaaag
    12121 taccatcgat tccagatgtc ccgtgtgtgg gaacatatcc agcatcgcca gtcgcgcaat
    12181 ggtatatcct gcttttaata acgcttcgct atcccgagcc agcgttgcag ggttacagga
    12241 tacataaact atacgaatag gttccagttt tataatttgc tgcataacac ctgcggcacc
    12301 tgctcgcgcc gggtccagca acactttatc gaagccgttt ttcgcccacg gctgctttgt
    12361 gacatcttct tcaagatttt cgtgataaaa cgtcacattc tgtaagcagt taagacgcgc
    12421 attctgctgg cctttttcca ccagcgccgg aacaccttct acacccacca cactggcagc
    12481 ttgtgtcgcc aatggtagtg taaagttgcc cataccgcag aacagatcca gtacgcgatc
    12541 ttcaggttcc acctccagcc attccaacgc acgcgctacc attttttggt tcacacccgc
    12601 attaacctga ataaaatcgc gcgggctaaa agttaagcgc aacccgtttg agtcatacca
    12661 gggcatctca ccagagacgg tttcgagtat ctcactatcg ggggcgagat acagatccag
    12721 gccttcagaa tgcgaaaagc gttccagttt ttcgcgatct gccgaactta gcggtgcggt
    12781 atggcgcaaa atcatcagcg tgccgctggt tgcctgtacc agttcaacat gaccaagatg
    12841 gcgcatagct tgtaagctgc ccaggcatgc cctgactttg ggcagcaatg cttcaagttg
    12901 gggcactaaa atgggacatt gtttaacgtc gacaatgtca ctggagcccg ctttgcgaaa
    12961 ccccatctga agttgctgtg ttttcggtaa gtagttcaga cttaaacgcg cgcgacggcg
    13021 atagccccag ggaacatcgg cgatcacttc agagacttcg tgtttcatta atcgggcgag
    13081 tgccgcactt ttgcttcgct gctgtaaatc cacgctggcg tgttgttgct gacagccgcc
    13141 gcatacgcca aaatgaggac agcgtggcgt ttcgcgttcc gggctatcgc ttaaccggcg
    13201 tacgacttta gcgcgggcat actgtttttt atcctcagta acagtaactt ccgcgttttc
    13261 ctgcggcaat aatccgggga taaatagcgt tttgccgtta tgtcgcgcca cgccctgacc
    13321 aaaagagtcg aggtcgttga ctgaaacggt tatgatctga cgcgtcgtcg tgcgtcgttt
    13381 tgcagagtag aattgcgcca ttggcgagac tttctcaatt taacagtgtg accttaattg
    13441 tcccataacg gaactccatg accaactaca gcctgcgcgc acgcatgatg attctgatcc
    13501 tggcaccgac cgtccttatt ggtttattgc tgagtatctt tttcgtcgtg catcgctata
    13561 acgacttgca gcgtcaactg gaagatgccg gtgccagcat tattgagccg cttgcagttt
    13621 ctactgaata tggcatgagc ctgcaaaatc gcgaatctat cggtcagtta ataagcgtac
    13681 tgcatcgtcg ccattccgat attgttcgcg cgatttcggt ttatgatgaa aataaccgac
    13741 tctttgtcac ctccaatttt catcttgatc cctcatcaat gcagctcggc agcaacgtgc
    13801 cgtttcctcg ccagctcact gtcactcgtg acggcgatat tatgatcctc cgcacgccga
    13861 ttatttctga gagttactcc cccgacgaat cgcccagtag cgatgccaaa aatagtcaaa
    13921 atatgttggg atatattgcg cttgagctgg atcttaaatc ggttcgcttg cagcaatata
    13981 aagagatctt tatttccagc gtgatgatgc tgttttgtat cggtattgcg cttatttttg
    14041 gctggcgctt aatgcgcgat gtaaccggtc cgattcgcaa catggtgaat actgtcgacc
    14101 gcatccgtcg cgggcaactc gacagccgag tggaaggatt tatgctcggc gagctggata
    14161 tgctgaaaaa cggtatcaac tcgatggcaa tgtcgctggc tgcttatcac gaagagatgc
    14221 agcacaatat cgaccaggcg acgtccgatc tgcgtgaaac gctggagcag atggaaattc
    14281 agaacgttga gttggatctg gcgaaaaagc gcgcccagga agcggcgcgt attaaatccg
    14341 agtttctggc aaatatgtca cacgagctgc gtacaccact gaatggtgtt attggtttta
    14401 cccgcctgac gctgaaaaca gaattaacac caacgcagcg cgatcacctg aatacgattg
    14461 aacgttcggc aaataatttg ctggcaatta ttaatgatgt tctcgacttc tcgaaactgg
    14521 aagcaggtaa gctgattctg gaaagtattc cattcccact acgcagcacg ctggatgaag
    14581 tcgttactct gctggcacat tcttctcacg ataaagggtt ggaactgacg ctcaatatta
    14641 aaagcgacgt gcctgataac gtgatcggcg acccactgcg attacagcaa atcatcacta
    14701 acctggtggg gaatgcaatt aaattcaccg agaatggcaa cattgatatt ctggtagaaa
    14761 aacgtgcgct gagtaatacc aaagtgcaga ttgaagtgca gattcgggat accggcattg
    14821 gtattcctga acgcgatcaa tcgcgcttat tccaggcctt ccgacaggct gatgccagta
    14881 tttcccgccg tcatggtggc accgggctgg ggctggtgat tacacaaaaa ctggttaatg
    14941 aaatgggcgg cgatatttcg ttccatagcc agccgaatcg cggttcaact ttctggttcc
    15001 acattaatct cgatctgaac ccgaacatta ttattgaagg gccatccacc cagtgcctcg
    15061 ccggtaaacg cctggcctat gtcgaaccaa actccgcagc agcgcaatgc acgctggata
    15121 ttttaagtga aacgccgctg gaagtggttt atagcccaac gttctccgcg ctgcctcccg
    15181 cgcattacga catgatgttg ttaggcatcg cggtgacctt ccgcgagccg ctaacaatgc
    15241 aacatgagcg attagcgaaa gcggtatcga tgaccgattt cctgatgctg gcacttcctt
    15301 gccatgcaca agtcaatgct gaaaaactca agcaagatgg tatcggcgcg tgtctgctga
    15361 aaccattaac acctacgcgc ctgttgcctg ccctgacgga attttgtcat cacaaacaaa
    15421 acacgctttt gcctgtaacc gatgaaagta agctggcaat gacagtcatg gcggttgatg
    15481 acaaccccgc taacctgaaa cttatcggcg cattgctgga agatatggtg caacatgtgg
    15541 aactttgcga tagcgggcat caggcggttg aacgggcgaa acagatgccg ttcgatttga
    15601 tcttaatgga tattcaaatg cctgacatgg atggcattcg ggcctgcgag ctcatccacc
    15661 agctccccca tcagcaacaa acgccggtta tcgcggtaac ggcgcatgca atggccgggc
    15721 aaaaagagaa gctgcttggc gcagggatga gcgattatct ggcgaaaccg attgaagaag
    15781 agcgattgca taatttgttg ttgcgctaca agcctggcag cggtatttcg tctcgcgtcg
    15841 tgacgcccga agtcaacgaa attgtggtga acccgaatgc gaccctcgac tggcaactgg
    15901 cactacgcca ggcagcagga aaaaccgatt tagcgcgcga tatgctgcaa atgttactcg
    15961 atttcctgcc tgaagttcgc aacaaagttg aggaacagct ggttggagaa aacccggaag
    16021 gcctggttga tttgattcat aaactgcatg gcagttgcgg ctatagcggt gtgccgcgta
    16081 tgaagaatct ctgccaactt atcgaacaac agctacgtag cggtactaaa gaagaagatt
    16141 tggaaccgga gctgctggaa ctgttggacg agatggataa tgtcgcgcgc gaagccagca
    16201 aaattctcgg gtaacggacg tcttgtcgag tagcaatcgg ctttttatga cgctgttttc
    16261 agattggtga caaagtgcct gtgtttatgc cggatgcggc gttaacgcct tatccggcct
    16321 acaaaaccaa ttaaattcaa taaattaaaa agcatgtagg cctgataagc gtagcgcatc
    16381 aggcaatttt gtgtttggca tcagtctcaa accggctcca gatagagccg gttttggttt
    16441 tttgtcttaa cgcaccatgc acgggcgctt gttatcgaac gtccagccag gaatcagata
    16501 ctgcattccc atcgcatcgt cacgcgcgcc aagcccgtgt ttctgataca gctcatgggc
    16561 tttcatcact tgatccatat cgatttctac acccagcccc ggtttttctg gcacctgtac
    16621 cagcccgcct ttgatctcaa acggttcttt ggtcaggcgc tgattacctt cctgccagat
    16681 ccagtgcgta tcaatagcag taattttacc cggtgcagcg gcggcaacat gggtaaacat
    16741 cgccagggaa atatcgaagt ggttgttaga gtgtgaaccc caggtcaggc caaattcatg
    16801 gcacatttgc gccacacgta ccgaaccttg cattgtccag aaatgcggat ccgccagcgg
    16861 gatatcaacg gattgtaacg agagcgtatg gcccatctgc cgccagtcgg tggcgatcat
    16921 attggttgca gtcggcaggc cagtcgcgcg acggaactct gccatcactt cacgcccgga
    16981 gaaaccttgc tccgcaccac acggatcttc tgcataagcc agcgaacctt tcaggtattt
    17041 gccgatttta atcgcttcgt tcagcgacca ggcaccgtta ggatcgagcg taatacgcgc
    17101 ctgcgggaag cgttgcgcca gtgccacaat agactcggcc tcttcttccc cggccagtac
    17161 accgcctttc agtttgaaat cgttgaagcc atatttttca tacgccgctt ccgccaggcg
    17221 caccaccgca tccggcgtca tcgcttcttc atgacgcagg cgataccagt cgcatgagtc
    17281 atccggctgg ctttgatacg gcagaggtgt ggctttgcga ttaccgacga agaacagata
    17341 accgagcatt tcgacttcgc tacgctgttg accatcgccc agcagggatg ccacgttaac
    17401 ccccagatgc tgccccagca gatccagcat tgccgcttct atcccggtaa ctacatgaat
    17461 agtggtacgc aaatcaaatg tctgcaaacc gcgcccgccg gcatcacgat cggcaaaagt
    17521 attacgcacc agcgtcagaa cgtttttgta ttcacccagc gttttaccca ctaccagcgg
    17581 aattgcatct tccagcgttt tacggatttt ctcgccgccg ggaatttccc ctacgccagt
    17641 gtgaccagaa ttatctttga taatcacaat attacgcgtg aagaacggtg cgtgtgcacc
    17701 actcagattc atcagcatac tgtcatggcc cgccaccggg atgacctgca tttcagtaac
    17761 aacaggcgtc gtaaattgag aactcatatt tatgtccttt ttcagaatca atgacggccg
    17821 aaaacgggac gtttacggtc aaaggtccag ccggggatca ggtactgcat cgggcctgcg
    17881 tcgttacgcg caccgccagg cagacgttta taggcctcat gtgccttttg tacctgttcc
    17941 cagtccagtt ccacgcccag accgggcgca tcaggaacgg caatttttcc gtttttaatc
    18001 tccagcggat tttgggtcag gcgacaatcg ccctcctgcc aaatccagtg ggtatcgata
    18061 gcggtaggat tacccggtgc cgccgcgccc acatgggtaa acatcgccag agagatatcg
    18121 aaatggttgt tggaatggca gccccaggtc agcccccagt cgtcgcaaag ctgcgccact
    18181 cggactgcac cggaaagcgt ccagaagtgc ggatcggcaa gtggaatatc taccgcattg
    18241 agcatcaccg catgacccat ttcgcgccag ttggtggcga tcatattagt cgcgacgggc
    18301 aagccggtcg cccgccgaaa ctccgccatc acttcacgtc cggagaagcc ctgttctgcg
    18361 ccgcatggat cttcggcata ggtaagaaca tcattcagcc ctttgcacag agaaatggct
    18421 tcatcaagca gccatgcacc gttgggatca acggtaatcc gcgcatccgg gaagcgtttc
    18481 ttcaatgcac gaacagtgtc gatttcttgc tcgccaggta acacgccgcc cttaagtttg
    18541 aaatctttaa agccgtagcg atcctgtgag gcttccgcca gacgcacaac ggcttcgctg
    18601 ttcatcgctt tctgatggcg caactgatac cactcatggt tgcccggcgt attttccaga
    18661 taaggaagat cggttttggt ccgatcaccg atataaaaca gataaccgag gacggtaata
    18721 gcatcgcgtt gcttgcctgg ccctaacagt tcgcagaccg gaacattcag cgccttacct
    18781 agcaggtcaa gcaaggcggc ttccagcgcc gccacggcgt taacgcgcaa ttcaaaggtc
    18841 caggcccctt taccgaaggt atcaaaatcg gctgcttgat tacctttatg cacctgctga
    18901 accactttat tcaggcgcgc aacttcctag cccagcacca tcggaatagc atcgaccaat
    18961 gtctgataaa tcacctctcc gcctggcgct tcaccaatgc cggtatgccc ggcgttatcg
    19021 gtgagtacca caatattgcg agtgaaatat gcgttatgcg ccccaccaat attaagcaac
    19081 atgctgtcgt acccggcgac cggaatgact ttcatatcag taataacagg actggattgt
    19141 gtcgtcatct tattgcccca cgacaggttt cagctcgata cgcttgatat cgcccaccag
    19201 caccaggtag ctcagtaccg cgattaaggc atgaacacca acataaatca gcgccccatt
    19261 aaacgagcca gtcgtgccaa cgatataacc aattgcgatt ggcgtgacga tgccagaaat
    19321 gttaccgaac atgttgaaca ggccaccgga aagaccgctg atctcttttg gcgcggtatc
    19381 tgccattact gcccaaccca gcgccccaat gcccttaccg aagaaggcca gcgccataaa
    19441 gccgatgatc atccactcaa cgttgacgta gttgcagaac accatcacca tcgacagcaa
    19501 catgcccatt acgatcggtg ttttacgcgc aatgttcagc gatcccgtgc ggcgcatcag
    19561 ccagtcggaa ataatcccac ccagcacacc accgataaaa ccacaaaccg ccggaacgga
    19621 agccacaaag cccgctttca gaatcgacat cccacgcgcc tgcaccagat aaaccggaaa
    19681 ccaggtaatg aaaaagtaag tcagggcgtt gatacagtac tgaccgatat aaacgccgat
    19741 catcatccgt gacccgagca gttgtttgat ctgcccccac ttcacgctga acggcacttt
    19801 aactttggtg ttttgctgat ccatattgat cagcgcgcca cccgcggcga tgtactccag
    19861 ctctttctga tttacccccg gatgttgatt tggctcgtgg atgactttca accagataaa
    19921 gctgatggca atgcccagac cgcccataaa gaagaagacg tgtgaccagc ccacttcatg
    19981 cgtcagccag cccataatcg gcgcgaagat caccgttgcg aagtattgag cggagttaaa
    20041 aatcgacacc gccgttcccc tttcctgcgc cggaaaccag gccgcaacaa tgcgactgtt
    20101 gccggggaaa gatggcgctt cagcaagccc gaccaggaag cgcagagtaa acagggcaac
    20161 gataatgccg aatccactaa agatatcgac gaagccttgc agcaaggtaa acatcgacca
    20221 gataaagatc gaccagaagt agacgcgttt tgaaccaaaa cgatccagca accaaccacc
    20281 agggatctgc ccgataacat aagcccatga gaaggcagag aacacatagc ccattcccac
    20341 gggatcaagg ccgatatctt tggccatttc cgaaccggca atagaaagcg tagcgcggtc
    20401 gccgtagttg aaggatgtga cgataaacaa catcaccact atccagtaac gagcattcgt
    20461 gcgtttttcc acactgctcg cagcctgact taaagaactc attgttgcac tcctgaaaat
    20521 tcgcgttagc cacgctcact ctggactgcg acatcgccag gaaatcagag gtgacgtagg
    20581 gtgttttttg ccgtttttat aggtcgttcg ccgaatacgg cgcgtgttta tatctggcaa
    20641 tagcagtata aaaagcgcgc catagcggct caccgtgcaa caacacaaca ttaatgcgtt
    20701 caatgaggcc cgattttggc attagccctg gacggtggaa tccacttcac ggaaatgaaa
    20761 acaagaacaa gaaaggaagg gttaaaacga agaaataaag aagagtatga aatggatcgc
    20821 ttgactccag gcaaacgcca gtaaaaatcc gcgctatgaa gcagttttta ctggcatttg
    20881 cctgaaaaga ttcgattcag caccgctaaa acgacattta ccgctcgctg aacatatcag
    20941 gacaacagcg tgccccactg ttcgacccac ggatttgatt ccgtttccgg ttccgggttc
    21001 tcactggcgt caatcaacag catttcgcca acccgctgag cgctctgttc ctgcagcaag
    21061 gcatcgaact gtttgccgcc attgcagaaa ttcacataac tactgtcgcc gagcgcaatc
    21121 acgccataac gcagattcgg ctggaagccc agactatctt tgattccctg aaagagcggc
    21181 acaatgctat caggaaggtc gccctgcccg gtcgtggacg taaccaccag aacatactta
    21241 tcctgatagg gcagccagtc gcttaattca ggatcttcaa ataccgttgc tttgtggccc
    21301 tgtgcggtca gaattgcttc tgcttcttcc gccactaaca gcgaatttcc gtacatggtg
    21361 ccgacaaaaa taccaatttc cgccataccg ttttctccct ggattaggaa cttctctctc
    21421 catcctgacc cgatggcgca ctgaactcaa ccctttcatt ttcaggaagc agaccgcgcc
    21481 agccaaattg tgataacgcc tgcatccagg tgtcgtccag gcccgcgtga atagtcagcg
    21541 gctcaccagt aaaaggatgc gtcaatgaca actgactggc atgcagcatt aaccgctgaa
    21601 gaccaaaatg ctcagcaccg ctgcgattct ggcgtaaatc gccatgtttg ctatcgccaa
    21661 taatcggatg acgcaaatgg gcaagatgtc ggcgaagctg atgtttgcgt ccggttttcg
    21721 gctccagttc caccaggccg tagcgcgtgg tcgggtaacg tccggtcgct accggcattt
    21781 ctacggtcgc cagaccgcga taatgcgtca ctgctggctg cgggccttta tcttcgcggg
    21841 caaatttatc agcgattttg tccagttctt ccaccagtgg ataatccagc accgcttctt
    21901 ccatcaacca gccgcgcaca atcgcatggt aacgtttctg gatttggtgc tgttcaaact
    21961 gttgtgccag cagccgtccg gcctcgccgg ataatcccat caacaacaca ccagaagtgg
    22021 gtcggtccag acgatgagca gtaaaaacat gctggcctat ctggtcacgc acggtttgca
    22081 tgaccactac tttctcgtcg cgatccagcc agctacggtg aaccagccag ccggagggtt
    22141 tatttaccgc aaccagccat tcatcctgat agagtatttc cagcattagc tcgcatcatc
    22201 cgcaaaaaga gcatccagtt tttccaactc agccagaata agcgcgcgtt gcggatggtc
    22261 cgtcgccagc gccatttcat aatagggtgc aacggcaaaa gcgcccggta acggctggtt
    22321 gttatttaac aaatcgtgca ttcgcgggat cagcacccac tgcaaccact ccagtggttc
    22381 catggtgtcc ataaagaacg gttgggtact attaaattga tgcggctggg gttcatcgtt
    22441 tcgccagtgc tgatgttcac gcagtaacgc ttcaagcgcc tgcaactgga gacgaacgcg
    22501 gtcatgagtg gtcataaagc aacctcaata agaaaaactg ccgcgaagga tagcaggaat
    22561 aaaaaaaggg agcactgtat tcacagtgct cccggttcgt ttcgcagcat tccagctact
    22621 tttgttgctc cctgctcatc cttgacaact tttcctctgg ccatgcggcc aatcgttcat
    22681 cctgaactat tgcttcctgc tcacaccacc ccgatgtgat acttcatcct gaagtgtccc
    22741 aggccttcct gacccaccga atcatcctga ccggttctca ttctccatcc tggaggtgtc
    22801 ctttaacgcg tcctgcgtca tcctcttcgc ttcatccaga agcctttccc tgaaacacca
    22861 tcctggtgtg tccttcagaa gtgtcatcat cctgatgttc acttcgttgt ctgactccct
    22921 gtcgacgaag atagaatcgt ctttttcgcg aactcttaca aggcgcttac agacaatgcc
    22981 ttaaggaaaa tttcatatga aagttaggaa ttaacaagca acatgttgtt tttaaatgaa
    23041 aataacgatt cttatctttt taagtatacg cttatttcct ggcgatctcc tacagagtct
    23101 gtaagagatc tctcacaacc agatggggta tttggattac agaagaggct taagttgatt
    23161 aaggaattcc gcaagatttg gggctaaatg cgtccgttta cgtgttccca gcgtctcttt
    23221 acacacttca ccggagagat tacacaccga gatgacctct aactcttctt ccagcgtcgc
    23281 gataaatagc gtgggtggca gttttagcct tttctgagtc accagatgac caatcagatt
    23341 ttcctgcacc cgacggaagt cgtcttcact ccaggtttgc agcaatgtca gtttgatatc
    23401 gccaaactgc gcgtgcatat ccccggcaaa ctgagtcgta taaaaggtat gaattgtggg
    23461 ttgtatcaca atatcaaaag cgcgttcaac cgcgtttaca ttttgttccc cggtaaacgg
    23521 ctggggttgc cagtatacgg catcttcagt agttgaaata atgcacggtg aaggaacgcc
    23581 gtacagttcc tcacttaacg gccagctttt atgctcttca tgccacgcat cgcagtagcg
    23641 tgcagtaaaa tctttcaggg cttgtgcggt caaatcgtcc actaatttct ctcttcactt
    23701 aaaccagata cacttgtcgt ttagtttatc tggtttatga cggtgaaaca tgtcttctta
    23761 tgcaaaccat caggcacttg cgggcctgac tcttggaaaa tcaaccgatt accgggatac
    23821 ctatgacgcc agcctactgc aaggcgttcc acgcagcctg aatcgcgacc cgctgggtct
    23881 gaaagcggat aacctgcctt ttcaaggtac ggatatctgg acgctgtatg aactttcctg
    23941 gctgaatgcg aaaggtttgc cgcaggtcgc tgtcggtcat gttgaacttg attacaccag
    24001 cgtaaatctg attgagtcga agagttttaa gctctatctc aacagtttta accagacgcg
    24061 ttttaataac tgggatgagg tgcgccagac gctggagcgc gacttaagca cttgcgctca
    24121 gggtgaggtt agcgtggcgt tatatcgtct tgatgaactg gaaggccagc cgataggtca
    24181 tttcaatggc acttgcattg atgaccagga tatcactatc gataactatg aattcactac
    24241 tgactatctg gagaatgcca ccagtggtga aaaagtagtg gaagagacgc tcgtcagcca
    24301 cctgctgaaa tcaaactgcc tgatcaccca tcaaccagat tggggttcga tccaaattca
    24361 gtatcgtgga cgccaaattg acagagaaaa actgctgcgt tacctggtct cattccgtca
    24421 tcacaacgag ttccacgaac agtgcgtgga acgcatcttt aatgacctgt tacgcttctg
    24481 ccagccagag aaattgagcg tttacgcacg ttatacccgt cgtggcggtc tggacattaa
    24541 cccgtggcgc agtaatagcg attttgtccc atcgaccaca agactggttc ggcaataaat
    24601 tttttctcaa ttttgcgtgc tggattcacg cagaaggttg tgaaaggtca tcaggcaggg
    24661 ctattgtaat caaagggaat gacgatattc gtcccataag gagtttttct tgattacaca
    24721 tattagcccg cttggctcca tggatatgtt gtcgcagctg gaagtggata tgcttaaacg
    24781 caccgccagc agcgacctct atcaactgtt tcgcaactgt tcacttgccg tactgaactc
    24841 cggtagtttg accgataaca gcaaagaatt gctgtctcgt tttgaaaatt tcgatattaa
    24901 cgtcttgcgc cgtgaacgcg gcgtaaagct ggaactgatt aatcccccgg aagaggcttt
    24961 tgtcgatggg cgaattattc gcgctttgca ggccaacttg ttcgcggttc tgcgagacat
    25021 tctcttcgtt tacgggcaaa tccataatac cgttcgtttt cccaacctga atctcgacaa
    25081 ctccgtccac atcactaacc tggtcttttc catcttgcgt aacgctcgcg cgctgcatgt
    25141 gggtgaagcg ccaaatatgg tggtctgctg gggcggtcac tcaattaacg aaaatgagta
    25201 tttgtatgcc cgtcgcgtcg gaaaccagct gggcctgcgt gagctgaata tctgcaccgg
    25261 ctgtggtccg ggagcgatgg aagcgccgat gaaaggtgct gcggtcggac acgcgcagca
    25321 gcgttacaaa gacagtcgtt ttattggtat gacagagccg tcgattatcg ccgctgaacc
    25381 gcctaacccg ctggtcaacg aattgatcat catgccggat atcgaaaaac gtctggaagc
    25441 gtttgtccgt atcgctcacg gcatcattat cttccctggc ggtgtgggta cggcagaaga
    25501 gttgctgtat ttgctgggaa ttttaatgaa cccggccaac aaagatcagg ttttaccatt
    25561 gatcctcacc ggcccgaaag agagcgccga ctacttccgc gtactggacg agtttgtcgt
    25621 acatacgctg ggcgaaaacg cgcgccgcca ttaccgcata atcattgatg acgccgctga
    25681 agtcgcccgt cagatgaaaa aatcgatgcc gctggtgaaa gaaaatcgcc gtgatacagg
    25741 cgatgcctac agctttaact ggtcaatgcg cattgcgcca gatttgcaaa tgccatttga
    25801 gccgtctcac gagaatatgg ctaatctgaa gctttacccg gatcaacctg ttgaagtgct
    25861 ggctgccgat ctgcgccgtg cgttctccgg tattgtggcg ggtaacgtaa aagaagtcgg
    25921 tattcgcgcc attgaagagt ttggtcctta caaaatcaac ggcgataaag agattatgcg
    25981 tcgtatggac gacctgctac agggttttgt tgcccagcat cgtatgaagt tgccaggctc
    26041 agcctacatc ccttgctacg aaatctgcac gtaatctccc ctcccccggt gacgtttgtt
    26101 gccgggtttt ctcgtttttg gtcacttact catcaactca tttcatttgt tatatgaatg
    26161 tttcttacca ccctcacgcg acaaatatca tcacagttaa tatgtcatac aatttatatt
    26221 gcaacgcaaa cgtttcccta ttttcataaa ccgtatttta tcgctatgaa aaagaaatta
    26281 tcgccatgat taactaaaag tattgatttt ttcagttcaa cctacatata ttgcgcgccc
    26341 cggaagaagt cagatgtcgt ttaatgggca aatattgccc ttaaattctc ttttactttt
    26401 gatttacaga gtaaagcgtt gggataatct atcttccaag tagattattg tatttgggat
    26461 caagatcact gatagataca taacttgtgt gtatctttcc gccctcaaat tattacggcg
    26521 gtaaatgatt aagccatcgc cgatagacag atttcatttt tacggtcagg caccttcccg
    26581 ggctgaactg gctaaaagct gaattatttg cattcctcca ggagaaatag atggaaacga
    26641 ctcaaaccag cacgattgcg tcgaaagact ctcgtagtgc ctggcgcaag acagacacca
    26701 tgtggatgct gggcctttac ggcacggcaa tcggcgcggg cgtgctgttc ctgccaatca
    26761 acgccggtgt tggcggtatg atcccgctga tcatcatggc tatccttgcg ttcccgatga
    26821 cgttttttgc tcaccgcggc ctgactcgct tcgtactgtc tggtaaaaac ccgggcgaag
    26881 acattaccga ggttgtagaa gaacactttg gtattggcgc aggtaaactg attaccctgc
    26941 tctacttctt cgctatctac ccgatcctgc tggtttatag cgtggcaatc accaatacag
    27001 ttgaaagctt catgtctcac cagctgggta tgacaccacc accgcgtgcg attctgtcgc
    27061 tgatcctgat cgtgggtatg atgaccatcg ttcgctttgg tgaacagatg atcgttaaag
    27121 cgatgagtat tctggtattc ccgtttgttg gcgtactgat gctgctggct ctgtacctga
    27181 tcccgcagtg gaacggcgca gcactggaaa cgctgtctct ggacactgca tctgcaaccg
    27241 gaaacggtct gtggatgacc ctgtggctgg caattccggt aatggtgttc tcgttcaacc
    27301 actctccgat catctcttct ttcgccgttg cgaagcgtga agagtacggc gatatggcag
    27361 aacagaaatg ctcgaagatc ctggcattcg cacacatcat gatggtgctg accgtaatgt
    27421 tcttcgtctt cagctgcgta ctgagcctga ctccggcaga cctggctgcg gctaaagagc
    27481 agaacatctc gattctgtct tacctggcta accactttaa cgcaccggtt atcgcgtgga
    27541 tggctccgat tatcgcgatt atcgctatca ccaaatcctt cctcggccac tacctgggcg
    27601 cacgtgaagg cttcaacggt atggtgatta aatctctgcg tggtaaaggt aagtctatcg
    27661 aaatcaacaa gctgaaccgt atcactgcgc tgttcatgct ggtaacgacc tggattgttg
    27721 ccaccctgaa cccgagcatc ctgggtatga ttgaaaccct gggcggccca atcatcgcga
    27781 tgatcctgtt cctgatgccg atgtacgcaa ttcagaaagt accggcaatg cgtaagtaca
    27841 gcggtcacat cagcaacgta ttcgttgtcg tgatgggtct gattgcaatc tccgcaatct
    27901 tctactctct gttcagctaa gtcctttcgc gccgctttcg ggcggcgctt cctccgtttt
    27961 aacgcgatgt atttcctatg attagcgtat tcgatatttt caaaatcggc attggccctt
    28021 ccagttctca taccgttgga ccaatgaaag cgggtaaaca atttaccgac gatctgattg
    28081 cccgtaacct gcttaaagac gtgacccgcg tggtggttga cgtgtacggc tcgctctctc
    28141 tgactggtaa aggccaccac actgatatcg ccattattat gggcctggcg ggtaacctgc
    28201 cggataccgt ggatatcgat tccatccctg gttttattca ggatgtgaat actcatggtc
    28261 gcctgatgct ggcaaacggt cagcatgaag tggagttccc ggttgatcag tgcatgaact
    28321 tccatgccga caacctttct ctgcatgaaa acggtatgcg cattaccgcg ctggcgggtg
    28381 ataaagtcgt ttacagtcag acttactact ctattggcgg tggctttatc gttgatgaag
    28441 agcattttgg ccagcaggat agcgcaccgg ttgaagttcc ttatccgtac agttcagcag
    28501 ccgatctgca aaaacattgt caggaaaccg ggctgtcact ctctggcctg atgatgaaaa
    28561 acgagctggc gctgcacagc aaagaagagc tggaacagca cctggcgaac gtctgggaag
    28621 tgatgtgtgg cggtattgag cgcggtattt ccaccgaagg tgtgttacct ggcaaactgc
    28681 gtgttccacg ccgtgctgcg gcactacgcc ggatgctggt cagccaggat aaaaccacca
    28741 ctgacccgat ggcggttgtt gactggatca acatgtttgc actggcagtg aacgaagaga
    28801 acgctgctgg cggtcgcgtg gtgactgcgc cgactaacgg tgcgtgcggg attatcccgg
    28861 cagtgctggc gtactacgat aagtttatcc gcgaagtgaa cgctaactca ctggctcgtt
    28921 acctgctggt agccagcgcc attggttctc tttataagat gaacgcgtcg atttctggtg
    28981 ccgaagtggg ttgccagggt gaagttggtg tggcgtgctc aatggcggcg gctggtctgg
    29041 cagaactgtt aggtgcaagc ccggcgcagg tgtgcatcgc ggcggaaatc gccatggagc
    29101 acaacctcgg tctgacgtgt gacccggtcg ccggacaggt acaggtgcca tgcatcgagc
    29161 gtaacgccat tgcggcagta aaagcggtga acgccgcacg tatggcgctg cgccgtacca
    29221 gcgagccgcg cgtctgcctc gataaagtta tcgaaaccat gtacgaaaca ggtaaagata
    29281 tgaacgccaa gtaccgcgaa acctctcgcg gcggcctggc aatgaagatc gttgcctgcg
    29341 attaatcgct ctccagaggc ctcgttttgc gaggcctctt cccgatttct catccagcca
    29401 tagcctgttc cagtatcgaa tgttaccctt atcgcctgat ctttaagggg gttatcgtgg
    29461 ctgttcattt gcttattgtc gatgcactga atcttattcg tcgcattcat gccgttcagg
    29521 ggtcgccctg tgtcgaaacc tgccagcatg cgctcgatca gctcattatg cacagccagc
    29581 caacccacgc ggtcgccgtt tttgacgatg aaaaccgcag tagcggctgg cgtcatcagc
    29641 gtttaccaga ttacaaagcg ggtcgaccgc caatgccgga agagttgcac gacgagatgc
    29701 ctgcattacg cgccgccttt gagcaacgcg gcgtcccgtg ctggtcaacc agcggcaacg
    29761 aagccgatga cttagccgcc acgctggcgg tcaaagtgac acaggccggg catcaggcaa
    29821 cgattgtttc gacagataaa ggctactgtc agttactttc accgacatta cgtattcgtg
    29881 attacttcca gaaacgttgg ctggatgcgc catttatcga taaagaattt ggcgttcaac
    29941 cgcagcagtt gcccgattac tggggacttg cggggatcag cagttcaaag gtaccgggtg
    30001 ttgcgggaat cggaccaaaa agcgccacgc agctgctggt cgagtttcag agtctggaag
    30061 ggatatatga gaatctggat gcggttgccg aaaagtggcg caaaaaatta gaaacccata
    30121 aagagatggc gtttctgtgc cgcgatattg cccgcttaca aaccgatttg catatcgacg
    30181 gcaatttaca gcaattgcgg ttggtacggt aacggcgtgc cggatacgcc gcaaacgtcg
    30241 catccggcat tatcacatca gcgcatttac caggcggtat ggtaaagctc tacaatatcc
    30301 tcaagcgttg cttcacgcgg gttgccaccg gtacaaacat catccagtgc cgcctgcgcc
    30361 agtgccggaa tgtcttcctt gcgtacacca acatcacgca aatgtggcgg aataccgaca
    30421 tcacggttga gagcaaacac cgcttcaaca gcggcattac gcgcctcttc caggctcata
    30481 ccttccactt tcacgcccat aacgcgcgcg atatcgcggt acttctcacc ggtaaagtca
    30541 gcgttataac gcatgacatg cggtaacagg atggcgtttg caacaccgtg tggagtgtta
    30601 taaaacgcgc ccagtggatg cgccatacca tgcaccaacc ctaaccccac attcgagaag
    30661 cccatacccg caacatactg cccgagcgcc atttcttctc cggcatcctt atcaccagca
    30721 accgatcctc gcagcgcccc agcaatgatt tcaatcgctt taatgtgcag tgcatcggtt
    30781 agcgcccacg cgccacgggt aatatacccc tcaatagcat gagtgagcgc atcgacaccc
    30841 gtcgcagctt tcagcgctgg aggcatacca tccatcatgt cagcgtcaat aaacgccacc
    30901 tgcgggatat catgcggatc aacgcaaaca aacttgcgcc gtttttcttc gtcagtgatc
    30961 acgtagttaa tggtcacttc tgccgcagtg cctgctgtgg tggggattgc cagaatcggt
    31021 acactgggtt tattggtcgg ggaaagtcct tccaggctac gcacatcggc aaactccggg
    31081 ttgttgctga taatgccaat cgctttacaa gtatcctgtg gagaaccacc accaatagcg
    31141 atcaggtaat ccgcgccgct attctggaat acaccgagcc cttctttgac gacagtaatt
    31201 gttgggttgg gcactacgcc gtcgtaaatc gcccatgcca gccctgcagc atccatctta
    31261 tcggtcactt tcgccaccac gccgcattgc accagcgttt tatcggtgac gatcagcgcc
    31321 ttctgataac cacggcgttt cacctcatcg gttaaagccc caacagcacc ccgaccaaac
    31381 catgccgttt cgttcagaat cattctgtta gccatcatcc ttctccttgt tgctttacga
    31441 aattactctt caatccgtaa cccataggtt ttgaatttct ccagcactac ggcaatctct
    31501 tcatcgctca gcactggcac cgggtccgta atcgccaggg tcgtcaggta aagttgcgcc
    31561 agcacttcaa cttcatgcgc cagccataac gctttttcca gattcacctc acaagcgata
    31621 agcccatgat gttgtaacaa agttgcctta cgatttttga gagccagcgc aacatgttca
    31681 gaaagttcgc gtgttccaaa ggtcgcataa ggcgcgcaag gaatagaatt accgccagcc
    31741 gccgcaatca tgtagtgaat agcggggatc ggtcggttaa gaatggaaac tgctgtgcaa
    31801 tgaacggcat gattgtgaac aaccgcgttg gcatccggtc tgctttgata ggccgccata
    31861 tggaaacgcc actcgcttga ggggagcttt ccttcctcat gtttaccgtt gccatcaata
    31921 aagacaatat gcgactccgt cagtttttca tatggaatgc ctgtaggcgt aatcagcatc
    31981 ccatcctgat aacgtacact gacgttcccc gctgtcccct ggttcagtcc caggcgggtc
    32041 atttccaggc aagtgtcaat aatctgacga gcaagtttat ttcgttccat tagctacctc
    32101 tctctgattc aaaacagggc aataatgttg ttcctttcac actattgaat tagccgttta
    32161 attacccacc atcttcttcc tgattaacaa gaaagaaatt cacaagctta tattttgtga
    32221 cctagttcaa ctaatcacag taaataactg caagttctct ttttataacc ccattaaaaa
    32281 tgaccgctct taaaaatatt tatcaaaacg gtcatttttc tattcaccca agccaggaat
    32341 gcccgttttc ggcataaaca attaatacgg tcatctggtt tgtgtttttt atgatttatt
    32401 ttctgaaacg ggcatgaaat ttcgattatt aaagtgatgg tagtcacata aagtcacctt
    32461 ccagctaata agtgtgaccg ccgtcatatt acagagcgtt ttttatttga aaatgaatcc
    32521 atgagttcat ttcagacagg caaatattca ctgatatgaa gcccgaactc gctagttttg
    32581 cacttttgaa aacataaccg attacgtgct taagcttctg aacctaagag gatgctatgg
    32641 gaaacacatc aatacaaacg cagagttacc gtgcggtaga taaagatgca gggcaaagca
    32701 gaagttacat tattccattc gcgctgctgt gctcactgtt ttttctttgg gcggtagcca
    32761 ataaccttaa cgacatttta ttacctcaat tccagcaggc ttttacactg acaaatttcc
    32821 aggctggcct gatccaatcg gccttttact ttggttattt cattatccca attcctgctg
    32881 ggatattgat gaaaaaactc agttataaag caggaattat taccggatta tttttatatg
    32941 ccttgggtgc tgcattattc tggcccgccg cagaaataat gaactacacc ttatttttag
    33001 ttggcctatt tattattgca gccggattag gttgtctgga aactgccgca aacccttttg
    33061 ttacggtatt agggccggaa agcagtggtc acttccgctt aaatcttgcg caaacattta
    33121 actcatttgg cgcaattatc gcggttgtct ttgggcaaag tcttattttg tctaacgtgc
    33181 cacatcaatc gcaagacgtt ctcgataaaa tgtctccaga gcaattgagt gcgtataaac
    33241 acagcctggt attatcggta cagacacctt atatgatcat cgtggctatc gtgttactgg
    33301 tcgccctgct gatcatgctg acgaaattcc cggcattgca gagtgataat cacagtgacg
    33361 ccaaacaagg atcgttctcc gcatcgcttt ctcgcctggc gcgtattcgc cactggcgct
    33421 gggcagtatt agcgcaattc tgctacgttg gcgcacaaac ggcctgctgg agctatttga
    33481 ttcgctacgc tgtagaagaa attccaggca tgactgcagg ctttgccgct aactatttaa
    33541 ccggaagcat ggtgtgcttc tttattggtc gtttcaccgg tacctggctc atcagtcgct
    33601 tcgcaccaca caaagtcctg gccgcctacg cattaatcgc tatggcactg tgcctgatct
    33661 cagccttcgc tggcagtcat gtgggcttaa tagccctgac tttatgcagc gcctttatgt
    33721 cgattcagta cccaacaatc ttctcgctgg gcattaagaa tctcggccag gacaccaaat
    33781 acggttcgtc cttcatcgtt atgaccatca ttggcggcgg tattgtcact ccggtcatgg
    33841 gttttgtcag tgacgcggcg ggcaacatcc ccactgctga actgatcccc gcactctgct
    33901 tcgcggtcat ctttatcttt gcccgtttcc gttctcaaac ggcaactaac tgaacatatt
    33961 ttccgaataa agtgaggaat ctgtaatgaa caaaatcagc ttaccgaaaa ttggtatccg
    34021 cccggttatt gacggtcgtc gcatgggtgt tcgtgagtcg cttgaagaac aaacaatgaa
    34081 tatggcgaaa gctacggccg cactgctgac cgagaaactg cgccatgcct gcggagctgc
    34141 cgtcgagtgt gtcatttccg atacctgtat cgcgggtatg gctgaagccg ctgcttgcga
    34201 agaaaaattc agcagccaga atgtaggcct caccattacg gtaacgcctt gctggtgcta
    34261 tggcagtgaa accatcgaca tggatccaac ccgcccgaaa gccatttggg gctttaacgg
    34321 cactgaacgt cccggcgctg tttacctggc agcggctctg gcagctcaca gccagaaagg
    34381 catcccggca ttctccattt acggtcatga cgttcaggat gccgatgaca catcgattcc
    34441 tgccgatgtt gaagaaaaac tgctgcgctt tgcccgcgcc ggtttggccg tcgccagcat
    34501 gaaaggtaaa agctatctgt cgctgggcgg cgtttcgatg ggtatcgccg gttctattgt
    34561 tgatcacaac ttctttgaat cctggctggg aatgaaagtc caggcggtgg atatgaccga
    34621 actgcgtcgc cgtatcgatc agaagattta cgacgaagcc gaactggaaa tggcactggc
    34681 ctgggctgat aaaaacttcc gctatggcga agatgaaaat aacaaacagt atcaacgcaa
    34741 tgccgagcaa agccgcgcag ttctgcgcga aagtttactg atggcgatgt gtatccgcga
    34801 catgatgcaa ggcaacagca aactggccga tattggtcgc gtggaagaat cacttggcta
    34861 caacgccatc gctgcgggct tccaggggca acgtcactgg accgatcaat atcccaatgg
    34921 tgacaccgcc gaagcgatcc tcaacagttc atttgactgg aatggcgtgc gcgacccctt
    34981 tgtcgtggcg accgaaaacg acagtcttaa cggcgtggca atgctaatgg gtcaccagct
    35041 caccggcacc gctcaggtat ttgccgatgt gcgtacctac tggtcaccag aagcaattga
    35101 gcgtgtaacg gggcataaac tggatggact ggcagaacac ggcatcatcc atttgatcaa
    35161 ctccggttct gctgcgctgg acggttcctg taaacaacgc gacagcgaag gtaacccgac
    35221 gatgaagccg cactgggaaa tctctcagca agaggctgac gcttgcctcg ccgctaccga
    35281 atggtgcccg gcgatccacg aatacttccg tggcggcggt tactcttccc gcttccttac
    35341 cgaaggcggc gtcccgttca ccatgactcg tgtcaacatc atcaaaggcc tgggaccggt
    35401 actgcaaatc gcggaaggct ggagcgtgga attgccgaag gatgtgcatg acatcctcaa
    35461 caaacgcacc aactcaacct ggccaaccac ctggtttgca ccgcgcctca ccggtaaagg
    35521 gccgtttacg gatgtgtact cggtaatggc gaactggggc gctaaccatg gggttctgac
    35581 catcggccac gttggcgcag actttatcac tctcgcctcc atgctgcgta tcccggtatg
    35641 tatgcacaac gttgaagaga ccaaagtgta tcgtccttct gcctgggctg cgcacggcat
    35701 ggatattgaa ggccaggatt accgcgcttg ccagaactac ggtccgttgt acaagcgtta
    35761 atacattact ccctgatgtg atgcccggtc gctccggctg ccgggcctga acaagcaaga
    35821 gtggttagcc ggataagcaa tgttatccgg ctatattgca ggagcgatca tgaaacaaga
    35881 agtgatcctg gtactcgact gtggcgcgac caatgtcagg gccatcgcgg ttaatcggca
    35941 gggaaaaatt gttgcccgcg cctcaacgcc taatgccagc gatatcgcga tggaaaacaa
    36001 cacctggcac cagtggtctt tagacgccat tttgcaacgc tttgctgatt gctgtcggca
    36061 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttggtgt
    36121 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    36181 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    36241 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    36301 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctctttattt cgtcgctgat
    36361 taaccaccgt ttaaccggcg aattcactac tgatatcacg atggccggaa ccagccagat
    36421 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    36481 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacagcgc
    36541 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    36601 gttcgccctt tttggcgctg gtgctgaaca aaatgaaccc gtgctctctt ccggtacatg
    36661 ggaaatttta atggttcgca gcgcccaggt tgatacttcg ctgttaagtc agtacgccgg
    36721 ttccacctgc gaactggata gccaggcagg gttgtataac ccaggtatgc aatggctggc
    36781 atccggtgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    36841 gttgattgaa gaggctcgtc tgatcgcgcc tggcgcggat ggcgtgaaaa tgcagtgtga
    36901 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    36961 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    37021 ggaaaaaatc gggcacttta aggcctctga attattgtta gtcggtggag gaagtcgcaa
    37081 cacattgtgg aatcagatta aagccaatat gcttgatatt ccgttaaaag ttctcgacga
    37141 cgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    37201 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    37261 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    37321 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    37381 ctcactttcc cgcccattcg atgggaccgc aggtgatccg cgctgatggc ctgttggtga
    37441 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgca ccgccgctgg
    37501 tgatgatggc agcggtagaa ggtgacactc tcgatcctga agtagaacga cgttaccgta
    37561 atgcgctttc actataagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    37621 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    37681 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    37741 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    37801 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    37861 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    37921 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    37981 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    38041 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    38101 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    38161 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    38221 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    38281 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgct atcaacgctg
    38341 attacaaatc aatgctatta aaacgcgccg cgcaatcgtt gttattgatt gataaaagta
    38401 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    38461 ctgatgaacg ccaggttgca acttctttgg taacaggctg acggaaaggg tagcaggccg
    38521 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    38581 gatacggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    38641 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgacac
    38701 ttcttcgtag cgttttttca tcggcagttt gaggttgaaa atggtttcac gacaccagcc
    38761 attaaccagc cactgcgcca tcaatgccgc aactttcgcc ggtttttcaa ccatatcgca
    38821 taccatccag gagatattgc tgcgcgtcgg acggaatttg aaaccgtctt cccgcagcca
    38881 cgtcacctgt ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    38941 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    39001 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    39061 tgcctcttcc agtttgagcg tggaacgact cggcgcatct gccggaaatt tcaggcgcgg
    39121 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcagcc
    39181 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    39241 cccggcatcg cgcaaggcag cgcgtagcgg cacggtgaat ttacggcaga atttcagcaa
    39301 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    39361 gccctgcaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaagag
    39421 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    39481 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    39541 cacgcgggca aaaccgaaaa tctcccgctg gccggcttta tcggtaattt ctgcggcgca
    39601 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    39661 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    39721 caaccggagt gacaaacgcc cacaaacgca gatgggacag tgccaggcaa taaaggctgc
    39781 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    39841 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    39901 cgaggccggt ctggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    39961 cgccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    40021 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    40081 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    40141 ctgcctggct gtcatgacaa accagataaa aagcgttttt actgaccaga acatcattaa
    40201 acgggcaaac aagacgtccg gcttcaattt cagattgcgc catcacgtta tttgccagcg
    40261 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatggcta aaaattggcc
    40321 cttgctgaac gttgatatga tttaacccca attgacgagt atatgtctgc cagtcacggc
    40381 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    40441 cgccagtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    40501 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    40561 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    40621 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    40681 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    40741 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    40801 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    40861 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt gaaacttaaa tggcgtgctg
    40921 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    40981 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    41041 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    41101 tcctgagccg gaacgaaaag ttttatcgga atgcgtgttc tggtgaactt ttggcttacg
    41161 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    41221 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    41281 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgatcca gttcgaccat
    41341 ctctttcacg tcggtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    41401 cataccggta tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    41461 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    41521 agaaataatt gcggcagtct ttttcatcat cttctcctta aagcgattaa taaatcttaa
    41581 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttactgacgc
    41641 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    41701 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    41761 agtttcgcgc ccagtttccc gctctacagg atgcgggcgt ctatctcgac agcgccgcga
    41821 ccgcgcttaa acctgaagcc gtggttgaag ccacccgaca gttttacagt ctgagcgccg
    41881 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    41941 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    42001 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    42061 cgggcgatga gattattgtc agcgtggcag aacaccacgc caacctcgtc ccctggctga
    42121 tggtcgccca acaaactgga gccaaagtgg tcaaattgcc gcttaatgcg cagcgactgc
    42181 cagatgtcga tttgctgccg gaactgatta ctccccgtag tcggattctg gcgttgggtc
    42241 agatgtcgaa cgtaactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    42301 cagccgggat agtggtgata gttgatggtg ctcagggggc ggtgcatttc cccgcggatg
    42361 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    42421 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggcg
    42481 gcggcaaaat ggttcacgaa gtgagttttg acggcttcac gactcaatct gcgccgtgga
    42541 aactggaagc aggaacgcca aatgtggctg gtgtcatagg attaagcgcg gcgctggaat
    42601 ggctggcaga ttacgatatc aaccaggccg aaaactggag ccgtagctta gcaacgctgg
    42661 cggaagatgc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    42721 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgctg ctggcggagt
    42781 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    42841 tgaccggcac actgcgcgcc tcttttgcgc catataatac aaagagtgat gtggatgcgc
    42901 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacga acccgcaatt
    42961 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    43021 gttgacgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    43081 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    43141 gctgggatat accgtggctg aaaacggtaa aatgcacttc tttggcgaca gcgaaggacg
    43201 tatagtgcgc ggcctcctgg cggtgctgtt gactgccgtt gaggggaaaa ccgccgccga
    43261 attgcaggca cagtcgccgc tggcattgtt tgatgagctg ggattgcgtg cgcaacttag
    43321 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    43381 ggtttaagca cagaaccggg tcggataaga cgtttgcgtc gcatccgacc tgattgttta
    43441 cccctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaacc
    43501 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgctttgg
    43561 tccttccgcc gtggctttca tcgcacacac cgtaccgtct gactgcggat acaccaatgc
    43621 ttcggtagag aacacgcaat ccacaccgag cttaccttta ctgtttttca ctacgccaaa
    43681 atcgcttttc aggcgctcac gcaacttcgc cgccagcggg tcctgaatcg ttttcgccag
    43741 atcggtaacc tgaatctgcg ttgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    43801 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    43861 ggcatcaatc acgtacgaat aacccacgct catatgctgc gctacgttat ccggcgtcac
    43921 gaaatcatcc accaccgtta cacagcactc cgagttaatc tggcgaatac gttccgccat
    43981 gacttccgct tttgccagcc caacgttatc gcgcagggca tgaatttgcc gattggtatt
    44041 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    44101 cgcttccgcc gcccaggaac cgacaccgcc aataccgacc acacaaatat gcgcgtcagc
    44161 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    44221 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    44281 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    44341 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
    44401 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcaccctgtc atatccgtaa
    44461 aaacggcata cagaatatca catcagccgc taaagacgtt acctgcgccc ggggcggttt
    44521 tcagcaccca gacacgtcca tagtggttgt accaacctgc gcggtgtccg gcttccggcc
    44581 cgatcccttg ataaatatcg aagtgttggc ctttgattgc gccaccgaca tccaacgcca
    44641 ccatcagacg caattcgtac tgaccgttaa atttgccgtt attatccagc agcggcactt
    44701 ctgccagcaa ggttgtacct ggtgggataa tggaacgatc agaggcaaca gaagcacggc
    44761 caaccagcgg caccgcactt gcccctttca ccggtgcaaa agattgcggt ttaaagaaaa
    44821 cgaaagacgg gttctgttcc agcagctcgc gaacctcggc ttcactgtgt gtttcgcccc
    44881 aatgacgaat cgcctgcatc gacatatctt cttttttcac ttcgccacgg tcgatcagca
    44941 ccttaccaat gctgcgatag gcatgaccgt ttttccctgc atagctgaaa aagttaagcg
    45001 gactgccatc accaaagtcg atatacccac taccctgcac atccataatg aaattatcca
    45061 tcagagagtt gctgtaagcg aggatgtact tatcgctcaa tgcacccgcg tagatctccg
    45121 cacgagacgg cagacgacca cgttttggcg gcatacggta aataggatac tggaactcgc
    45181 cctggcgggt atggcgcgcc tgaattaccg gcgtgtaata gcctgtaaac tgcacgttac
    45241 catagttgtc ggcaccttcc atctgccagg catcaatgcc gaactggcgc atattgcggg
    45301 tatcaccgcc tgcgcgcagc cactcttgca ccgcgttata aacattactc tggttgccat
    45361 acagacgcgg tgacgaatta cggatatggt taatttgctc ggcaaaatca ccggcgttaa
    45421 tcggcgcgcc aacggcatct ggctggttca ccagagagaa aggctgggta aatttcccgt
    45481 ctttatattg ctgtccgcga tcggttggtt tggaagagca ggcggcaagc attgccacaa
    45541 ccgtgcccat aagaaggtac tttacccaac gtcctttcat tgttctcttc ttcaggttaa
    45601 aaaataaggc gcaacgaaga taacaaacca ccgcgtttaa tgaaatgagc agcctctccc
    45661 tgacgcaaat tttgcacaaa aaataggctt tagtgatttg tttttgttca aaatcatgcc
    45721 agatccgtga tcggggtaaa aaaaaggttg catgaaaacg cgagcggagt atagtgcgca
    45781 tccacggacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc
    45841 ggttcaaatc cggcccccgc aaccaattat tgaacaccct aacgggtgtt tttttgtttc
    45901 tggtctccta taaaaaagcg ccattcagcg cctttttatc atccccttct cgccagggtc
    45961 gccccatcgg caaaatacgc tttaattccc gcaagaatag actccgcaac ttcctgctgg
    46021 aaggtcgccg ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc
    46081 agaatggagg gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt
    46141 ttatgcagct tgttgatttt accgagctta ttcagcaccg ctttaccaaa cttaaggctg
    46201 tcagcaatgg tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg
    46261 cttttgctca cgccaccaat caagtcggag gcgttctggg tttgtgccag atattttgcc
    46321 gcagtactgg ttgcaccttt ggttgagagc gcaaacaccg aggaaccgct cggctggcga
    46381 ctggtaaagg cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgagctttt
    46441 gctacgcgca cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata
    46501 ttgccctctt tctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt
    46561 tcgcgcgttt tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc
    46621 ataatgacaa tcggacgatc gcgccctgct ttacctggtt gtggaccact ttgcgctggc
    46681 ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc
    46741 tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg
    46801 accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatccgt
    46861 acggtttgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga
    46921 atttgtgccg ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc
    46981 acgcgttccg ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc
    47041 gtcacgcggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctgaccgca
    47101 gccagactga cctgacttac gctcaatagc cacatggcac ccgcgccttg cagtaaacga
    47161 cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc
    47221 tgtgtaacaa gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc
    47281 atctaataaa cggtaaacat tctttttata ttcacgccat tactgataaa aaagtcgctc
    47341 tcgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca ataaattcga
    47401 ataataatgc aaagaggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat
    47461 tccgccattc ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg
    47521 gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc
    47581 acagcctcgg catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc
    47641 tggctgcgca tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa
    47701 cactggaact ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt
    47761 cgatgagtct caataacacg ccgctacagg gcgcgcatat caacgtcgtc agtggcaatt
    47821 ttattattgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta
    47881 tccggcggat tgatgaagac gcgatccatc gtcaactgga cagcggtgca atagtgctaa
    47941 tggggccggt cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg
    48001 ccactcaact ggccatcaaa ctgaaagctg agaagatgat tggtttttgc tcttcccagg
    48061 gcgtcactaa tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcgc
    48121 gggtagaagc ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg
    48181 gcgcagtgaa agcctgccgc agcggcgtgc gtcgctgtca tttaatcagt tatcaggaag
    48241 atggcgcgct gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg
    48301 aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat cggcggtatt ctggagttga
    48361 ttcgcccgct ggagcagcaa ggtattctgg tacgccgttc tcgcgagcag ctggagatgg
    48421 aaatcgacaa attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct
    48481 atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc
    48541 gcagctcatc acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg
    48601 gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat
    48661 ttaccccggt ggatattgat ttattgcccg aaagcaaaaa gcagttgtac aactaccagc
    48721 gtaaatccaa agtgttgatg gcggatttag ggtaagcgat gaaaatcgtc ggatgcgaca
    48781 tgcgtaacac tcgtacgtcg catccggcaa ttacgtttat tcccgtgaac taaacaacgc
    48841 cgccagacca ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tgcgctcatc
    48901 ggcatacagc gacagacggc gacgcgcgcg ggtcaccgcg gtataaacca gctctcgcgt
    48961 tactaccggc gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg
    49021 cgatttatgt accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac
    49081 agacttaata ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag
    49141 cgcaataccg atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac
    49201 cggtcggcct tcgtaccaac gagagtgcgg attacgatga attttgcgct tctgttgcat
    49261 aaactgctca attcgctcat tcagtccagc cacgccaaat ggcccttccc gcagggcgca
    49321 caaaagttgg tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag
    49381 cagatccaga taacgtccat aacccgcaag agcttcctca agcatcgcaa tataatcttc
    49441 gccgctctgt aaaagccgtt tttcaatatc agtaaaatcc tgctgaaaaa cggttttcac
    49501 tgccgtttta tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc
    49561 gaaacgatag cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc
    49621 agttcctgcc ggaacgtgag ttcccgtcag gcggcttagc tgcccggcac gctcggcggt
    49681 aaagccggca ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc
    49741 cagttgatca cgatcgccga gaaagatcac tcgcgcatga tcgggcaagg cgtcgatcag
    49801 tctcgacatc ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg
    49861 cagcgggtta ccggcatgat gacgtaaacg ctggctaccc ggctgcgcac ccagcaatcg
    49921 gtgcaaagta ctggcatctt ccggaatacg tttcttttgt tcatcggtca gcggtaactg
    49981 tcgcaaagcc ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc
    50041 cagacggata cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc
    50101 tacggtggtc gttttaccgg tgccagggcc gccggaaatc accgagatcc gccgcgtcag
    50161 cgccactgct gccgccactt tttgccagtt aatttcatca ccagtcggaa agagtttatc
    50221 cagggtttgc gccaacaagg cttcatccac ctcgatggca tgattcacct cattgaagaa
    50281 gcgcgctacc gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag
    50341 gatcatcggc gtgggttcat ctccccggct gaccgcttgt gaagccagca agcattcttc
    50401 ccaattttgt agctcaccga tttcactgac acaggtcgcc aacagcggat gcgactcctc
    50461 attattttcc agtcgtgaaa gcggcaaaca aacgtgcccc tctccggcat catgacttaa
    50521 cagtgccgcc gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaactg
    50581 cacgtccagc gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt
    50641 cattacgcct cctccagagt cataccggca aacatctcat ccatcaggtc aatcaacccg
    50701 gcgttggggc gggttgtgta aatcccttgt tgcggatgtt ctttatcaac accacgcagg
    50761 aacagataaa taacgccgcc aaagtggcgc tcatagtcgt aatcagcaat gcgatggcgc
    50821 agataacggt gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc
    50881 attgccgctg ccatagcctg ttgggtgtaa gccgaactat cttcacccaa ccagttggat
    50941 ttatagtcga gcaggtaata acgcccttcg tggcggaaca ccaggtcgat aaagcctttt
    51001 aacatgccac gtgcctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac
    51061 tggcgaatta gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac
    51121 tccatctcca cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg
    51181 agaggtgcct ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca
    51241 aagccgccga gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaattg
    51301 aggtcttcaa acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc
    51361 ggtgttaacg tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt
    51421 ggcatcagat cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca
    51481 cgccagttat cgccgggcaa tcgttgtagc gtcctcgcat tcagttctgc agttaaagca
    51541 tcattaacct gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca
    51601 tcgcataacg cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc
    51661 agcaaacgcc cgagggcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg
    51721 cgcaccagcg gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa
    51781 agcaaacgca gatcttccgc cagacgttcg acctccgcaa gatcgacgct ttctggcgca
    51841 gcgttaagat ccagaacagc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg
    51901 acgcggaaat tggtaataaa tggcaaccag accagcggat attccagccc ttttgattta
    51961 tggatcgtga caatctgtac cagatgttta tcactttcga ggcgcatttg ttggctggag
    52021 gcattactgt ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt
    52081 tccagctgcg ttccggcttc ttgtagcagt tcgctgatat gcaagatatc ggtaagacgc
    52141 cgctcaccgc ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc
    52201 cgcatcatcg gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg
    52261 accaccgcgt cccacgcatg ttcgtcatta ttcagcgttt cgatatccag cgcgttcagc
    52321 cccatcattg acgttgccag cgcactacgc agggtgttct cacgttcggg cgtcatcacc
    52381 gcctgcaaca accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc
    52441 gaaaggtaaa cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc
    52501 tggcggctgc gcaccagcac actgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg
    52561 ttcatcagca acgcttcgcc ccgctgtccg gcttgtagcc agtcgcgaat ttgcgcagca
    52621 cttacctgcg ccatggtact ttgatagtcg ccaacgccgc aactttcgcc ttccatcagc
    52681 cacattttca tcgcaggctg cgtttcacct ttaaagacaa aacgtaacgc ctgatttttc
    52741 ccggctgatt tcactggaat aaacggtatt tcgcgaaaca tgaacgcgtc atcagtctgg
    52801 ctgaaaagct tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa
    52861 gtgtagtggg cgtgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg
    52921 aatgcatata tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg
    52981 tgccagatac ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc
    53041 attgccaccg ggaatcgcgt acggatcgcc gctgccaaca cctcaccgct ttcgctacgc
    53101 agcgcggaat cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt
    53161 tcacgcgcta ctgtttcgcg gatctcagcc aatgcgcggg tgatcaccag atcgcggatc
    53221 gacaatggtt ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc
    53281 cccccggcct tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc
    53341 aactgataac tgtttctctc ttcttctgcc caggcgctga tcttgtcgat ccatttagcc
    53401 tgattgctac ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt
    53461 tcacccactg cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg
    53521 tgacgggaag ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cgcttcacct
    53581 tgcagataac gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg
    53641 acctgggcta tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc
    53701 tggtaacgta gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca
    53761 aaggcattca ggttgagcat gcgctggcaa aaaccgtgaa tagtaaagac tgccgcttca
    53821 tccatctgcc gttcggctaa caacaaccac ttcgcggctt gcgctttatc gtcgatctct
    53881 tccagcagac gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac
    53941 tcatggatat tgctacggat acgaccacgc aattctgccg tggcagcctc ggtaaaggtg
    54001 accaccagca gttcttcaac ggtcagcggg cggggaaagg cggcggaacc gcctagtcca
    54061 agtaacaggc gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct
    54121 tcaatcaggc gctcgccctg aaagggcaag cgcaaaggat ctagtgtctc ggcgacatca
    54181 ctcattcttt tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac
    54241 tttccagcct tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga
    54301 cagaatagcc atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg
    54361 cggcgtcagc agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc
    54421 gcgatcgaaa tctttactta acttcaatgc ttcttcgccg agcgtttgcg gtgcctgcag
    54481 catctgggta attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa
    54541 ttttgcctct gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt
    54601 atcattgctt tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc
    54661 aaacacggca tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac
    54721 gatctgcccc aacagagagc tataggctga gcttgtgtat tcatcgtagc cagtcggtac
    54781 aaataccgct gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg
    54841 ttttttatcg accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg
    54901 tttttgcaca tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat
    54961 aaactctggt cgagcccctg attttaaggc gtcacgataa gccagcacct ctttcaacgt
    55021 aatggagggc aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat
    55081 ctgcgcgggc ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg
    55141 gttataccag gacttcgcct gctcaagctg atcttccgta gcggtatagc taaagtatcc
    55201 ctcgagcaat gcctggaaca gctgcggcag acgctgggtg taaccattag cattaaccat
    55261 aaggccgttg ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa
    55321 ctgatcaagc gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc
    55381 gctgtccatg gctttcggat tacgcaaaat caggctgaca tcagctttgg gttcgctgga
    55441 aaaataacgg cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg
    55501 atggtcatat ttcttctctg acttaatcag cgagaaatcg tcaggaatat aggggttaag
    55561 ctccggtaag gagagcgcaa tattggcagc tttttgctgc cagtcagcga aagtttgttc
    55621 gctgatttta tcgacctgat acggcgcatc gacaaaataa gccgttttat tgtgcggctc
    55681 tttcgggctg atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcttt
    55741 tactgcttta gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg
    55801 aacgcgaatc atggtatctg ccagccattc gacgtaatcc atatcacggg tgattgacgg
    55861 ataacggaag tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcgatccc
    55921 tttttcacgt aacaaattga ggtagctaaa aatagccgcc acaacctgat cgcgattcgc
    55981 cagaccttta tcggttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat
    56041 aggatcggag ttggcgctaa tgccctcaac taacccctgc ttttgcagcc agtcagacag
    56101 tgtacctggg ctgcgattac caatcagata ggtaatcaat tcatccgttt tactgcggaa
    56161 cttcgctgag ttgttatcga tgcgaaactc aacgcgcaga actttacgcg gcagcgctgg
    56221 gacgtaatga atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg
    56281 ttttttgctc tctttgttcg gcacgcgacc aaaagtgtcc gccgccattt ttgccaactc
    56341 cggcagcggt ttattactgt aaataaccgc cttcatcaaa ttggcggaat agtacttctc
    56401 gtggaaatct ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag
    56461 gttaccacca gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg
    56521 tgccatgcgc atcccgtcac gtgtacgcgc catggttaat tcagcgttca ctgcattacg
    56581 ctcacgttcg gcgtatttct tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc
    56641 taccgcaccg ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc
    56701 agtgctggca ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg
    56761 cgggtacttt ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta
    56821 cgcctcggga tcttccagcg acccaacggg caccaccagc gccgatagcg atttaactgc
    56881 ctgcggatca gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg
    56941 gcggttatct ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc
    57001 ctgacttaag ggtgcccaaa gggcaactaa caacaataat gctttgaacc aggtgctgcg
    57061 gggcattcac ggacctcata atcaacttta ttttctggcc agattcaaca ggttaacgca
    57121 aatcatctgc cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc
    57181 agtccgtgac tggtgcgcat cataaagtaa gcggatagat tgcgcaattt ttatacagcg
    57241 ctcatgactg attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgattg
    57301 cctccattgt ctctggtgtt aattgccgcc agagcctttg ataccagata tcatcacctt
    57361 cgccactcac catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca
    57421 acgtggaatc gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg
    57481 cgccgccact ttcaggtaac accagcaatg gcgcggacat cccttcacga tacccctcaa
    57541 tcagttgtga gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcgc
    57601 cgtctttgcg tagaaaaagg cgactttcac cattaccacc gctggcacag tagacaaggt
    57661 gttccagcca aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca
    57721 agccatccgg ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caagcgagat
    57781 caatttccat actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca
    57841 tctcctggca ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg
    57901 ccgcccggaa gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata
    57961 actgctgatt gatttgataa cggctaagtc cttccagaat aaatggctcg gtgtcgggga
    58021 tttcgctgtc ttcagtacgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg
    58081 gatgtgccca gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca
    58141 gcggctgaac aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac
    58201 gagcatagct ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat
    58261 ggaggcaagt aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat
    58321 cgcccggtag ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg
    58381 ggaaacgttc actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct
    58441 gcgcggaaat taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc
    58501 cacgcttcgg tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa
    58561 cgccgtcgtt cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca
    58621 gagtacaaat gttaaccggc ccggcgagaa aacgctggct gatacgttct tgatccaggc
    58681 gctgtgccag ttcatcacgc aatagtgaca gcggcaccgc atcgccatac tgcgcaccta
    58741 aaccttcggc gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt
    58801 ccgcatccgg caggaagagg gcgttgagca tatcgcgaca aaccggcaac cactcttcca
    58861 gcggacgctc ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca
    58921 gatgccccac cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc
    58981 actcgccctg cgcgctctcc atcgcgtagc ccaacaacat acgcgtcagg ccaaatcgcc
    59041 aggtgtgttg tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc
    59101 aacgaatgcc ggattcgttg acccactgac gtaaataacg cagcccttct tcggtgatgt
    59161 caaaccgcgc cgccagcacc ggcacatcca gtaatgccag cacgtcttcc gacacaaagc
    59221 ggctgtctgg caacgataac aggctgataa acgcttcaag tacaggatgc gactgccgcg
    59281 cccgacggtc ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacacag
    59341 cctgaataaa cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag
    59401 taagtgtcgg gtcttcttcc agcatcgcca gcaggcgatc gtgtaaaact tcaacttcac
    59461 gctgcgggct atggcaaacg tggaaggtga tactgctatc cagtggatca agcggacgtt
    59521 tgttatcgct acgggaaaac tcttcgatgt tcacaccagc aacggcgcgg ttttccagtt
    59581 ccagaatgtc agactgaatg ttatgcagca ggttatctgg cgtcacatcg acaaaggcgt
    59641 ccagctcctg gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttgc
    59701 cccatgaggc cagcagcggg ttgccgacat cctgttcacc atcgctgtta aagagctgcc
    59761 cggcattttc gctgtcgcga aatagcggta attcgcgatc ttcaaaactg tgtcggcgct
    59821 ggcgagtcag tagtttcgcc agataagcag gatctttaat gtcgccccag taataacggc
    59881 aggggttggt aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga
    59941 gataaacagg cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg
    60001 ggcaggtcgt cgcggactcc agcgtttcga taaagcgctg atagagattg gcgcggtgcc
    60061 agcgcggttg tccgagttca tgggtatatt ccaccagcgc cttccacaac ggcgcttgcc
    60121 aggcctgtgc ctctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt
    60181 ccggacgata gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca
    60241 gctttcgctt gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct
    60301 ccagcaattg cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc
    60361 tctctttggg gatctccggt aacacccgga cgaacatatc ccagataaag ctcgcaggca
    60421 gcggaaaatc aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact
    60481 gtgccatacc ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt
    60541 cgcgttcgac aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga
    60601 cccttaacat agcggctcct gactactgac gattcgggca atgcaggcgg gtcatctcgc
    60661 ctgctctgcc cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg
    60721 tctgcattcg gttgacctgc cagttggcag gtggcgaaat cgcgcgcaat tgcgtttgct
    60781 gccaaccatg ccgccagagc tgttggtact ggtttctgct ggtaagactg ttcattaatg
    60841 ttcgctggaa acccgataac gcagtgacaa tcatcaccat caacaccatc gccaacatta
    60901 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataacg ccccctcttt
    60961 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc
    61021 gcctgttcgc cacaacgaaa cgccttcata accggcaatt aataaggctt cattatcttc
    61081 cagtaaacgc aaacaaaccc gggcaccagt ggcagcgtac agtaagcact gaactgcggg
    61141 ctgcgtctgc caggagtgca ttttccccca cgccaacgcc gactgaacga tggcctggcg
    61201 gcgcagtgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctggctcat
    61261 tccttgtaat agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac
    61321 acccttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt
    61381 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    61441 acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    61501 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccaacac ttgctccttc
    61561 agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat accgttactg
    61621 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    61681 gtacagatgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    61741 agccagattt catcttccag tgccagctta cgggtgctcg ttaaactttc acgctgtaac
    61801 gcaggcagaa agcgtgcagc ccccaacaac aatacgctac tgatcgccat agcaatcaac
    61861 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgac
    61921 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    61981 agccaatatg cccggcccat gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca
    62041 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    62101 aggtattcgc cccagcaacg gaactcacaa ggcaccataa cgtcccctcc ctgataaaac
    62161 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    62221 gcgcctggct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga
    62281 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    62341 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    62401 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    62461 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    62521 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaatgccc ggatgtggat
    62581 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    62641 tgataatcaa cttcggcagc ggacgcggtt cacggcttaa ttgcagatga gtttgatcca
    62701 tatgattact gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat
    62761 cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc
    62821 cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    62881 agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    62941 acgctgaaac aataatgcgg cgggaatccg ggtcgttttt cagctggttc agtaccgtag
    63001 tgatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    63061 ctggtccgag gtcgccgttt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    63121 gtagataagc aatgttagtg tcgccctgca gaaaccacag cagttcatgg atgatggaac
    63181 gcaggtggca acgtttagtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    63241 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    63301 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    63361 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    63421 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    63481 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagccagaca
    63541 cggctcccat cgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    63601 cttccagcag cagctcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    63661 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    63721 ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa
    63781 agttgcccaa acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaaa
    63841 cctggaagaa ggaacgttta gtacggcggg caaagataat catcaccacg ataacgccaa
    63901 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    63961 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    64021 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagccg ctgcccggac
    64081 gattcgcccg tcgtgttgcc agccacattg caaaaatgaa acccaccaga tacatcaggc
    64141 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    64201 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    64261 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    64321 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    64381 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tggcgaactt
    64441 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcataatcaa
    64501 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    64561 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    64621 ggagatcgat tccatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    64681 caggatgaag actgtcataa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    64741 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gacccgcttt gccagatgcg
    64801 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt
    64861 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt
    64921 caacttcatc gaggcttgtg accatcggca acagaatatt caggttgccc gtagcggcat
    64981 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    65041 gaatcccacg ccaacccagg catggattct cttcgctaat cggcatgtaa ggcagctgct
    65101 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    65161 tcccctgata ctgcgccacc tgttcttctt ccgacggaaa accactttgc agcatgaatg
    65221 ggatttcagt gcgataaaga ccgatgccat caatacggct gcccagtttt tcttcatgtt
    65281 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    65341 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    65401 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcga
    65461 cgatcagcgt ccgacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    65521 tccccagcgc acgtaccatg atcgcagcat gggagttggc tgcgccatct cgcacgacaa
    65581 caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    65641 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    65701 gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc tttgagatag ttatcgctta
    65761 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccat tctgccaccg
    65821 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    65881 agtaaagatc gaaaatagcc gccgtttctt tttgtgcacc ggcggcaaag cgtttgctgt
    65941 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    66001 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    66061 agccttcggc aatcgctaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    66121 attgcccaaa caaggcagtc aactgcgact gagaaagaat cgctgccatc tgggtggcaa
    66181 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    66241 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    66301 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    66361 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaag gcgagcgtta
    66421 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    66481 catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    66541 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    66601 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    66661 tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta
    66721 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    66781 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc
    66841 cgctcaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    66901 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    66961 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    67021 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    67081 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    67141 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    67201 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta acaacaattg
    67261 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    67321 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    67381 cattctgaat aagcccgata tagacatttt aaaatattca ttattagtca atgcattaca
    67441 tgtaatttaa gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    67501 ttggcgaaag atcaaccaat gccgtattta tccacagaat gtgccactaa gttaagcact
    67561 gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga
    67621 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    67681 cagtgaccag atttggaaaa cccgttgcag tgttgcgcaa ctcgattacc ggcaacctaa
    67741 aaagcgatat aaatcagata attgaacaac gcatgtggaa aaagttacac tgcgaatatt
    67801 cggcacataa ttgctgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc
    67861 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    67921 ttatacattg ggagaactgg cggcacttgc cgggctggtt acgccagaga atttaaaacg
    67981 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    68041 acctgaacaa gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    68101 tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg ttaacgggca atagcggagt
    68161 gacctgggaa accagccacg tgcgccacaa actcaaacgc gtactgtgga taccggttga
    68221 aggcgagcgc agcatcccgc tggcgcagcg tcgcgttgga tcaccgttgc tgtggagccc
    68281 gaatgaagag gaagaccggc agctacgcga agactgggaa gaattaatgg atatgattgt
    68341 tctcggtcag gttgagcgga ttaccgctcg tcacggggag tatttacaga tacgaccgaa
    68401 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgca cggggcgaac ggattctgac
    68461 gctgccgcgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    68521 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    68581 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcctggctt
    68641 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    68701 tctctggtgg tggcaaagct ccccacagca caacgtgctc atgcgcgccg tctggggttg
    68761 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg
    68821 acgaatccgc tttttacaat attcagtcag gaaatttctg cccgtgactt gattctgctt
    68881 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    68941 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    69001 ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac
    69061 ctgtttatta tgatggccgc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    69121 tcgattggtg attttgtcga gcgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    69181 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    69241 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacctgat tcgcaacaaa
    69301 aagaacccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca cttctgttac
    69361 cctcacagtt taagattttc ctgctttcaa gatatataac gtcagtttat aaacagacta
    69421 ttatcatagg tgggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    69481 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    69541 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    69601 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaataa
    69661 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    69721 tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat
    69781 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    69841 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    69901 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    69961 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    70021 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    70081 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgtct
    70141 gcagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccaa ccaactgctt
    70201 cggcaaactc ggttatagct ggacagattc tgcgcctgcg gtttcgctgc tggatacgct
    70261 ggacgcactg gcagagtacc aacgagcggg aaaaattcgt tatatcggcg tgtcgaacga
    70321 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    70381 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    70441 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    70501 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    70561 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    70621 cagacgtcat ggcctggccc ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt
    70681 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    70741 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtgc atcaggttta
    70801 tacttatccg gcaccataag attaacgccg gttaaaaccg gcgttaaata ttaatgacgg
    70861 cgctgccaga tccacagcgc cgttattgcc agcgcaaata gcgcaccgaa gccaatgcca
    70921 atgggcacga ccgggatgcc taccattacc gccagcgagt aaatgcccag catcaacaac
    70981 atggcgctgt tttcgccaag gttttgtact gcaatcgcat tccccgcccc gacgcttttt
    71041 ttaccccgct cctgtagcaa cgcattgagc ggaacgacaa aaaagccccc caacacgcca
    71101 atcagcatca acaaggcata ggctggcagc tgctcgtgtt gcagggagaa aatcagtacc
    71161 accacgccaa tcaaaattcc ggctggcata cagcgtgaca cggtttccag cgtaaccagc
    71221 ttcgctgccg cacctgcgcc aaccacgata ccaatcgcta ccatcgcgtt gagataggtc
    71281 ggcgtagcgt tatcggtaat gcccagcgcc accggtaccc acagcaccaa caggaaacgc
    71341 agcgtgacac ccgctcccca gaataaactg gtgcccacca gcgaaaaacg cgtttcacca
    71401 ttgcgccata gcgaggtgca ggcattcagg aaactgcggg tcatgttgat gagattccag
    71461 gactgccccg gacgcgccgc cgccagtttg ggaatgtaga tattggcaac gaccgcacca
    71521 ccgtaggcca gtgcgcatgc ggccagggcg accaggacat gccagtcagc cagcacacca
    71581 ccggctacgg aaccgagcaa aatcgccgct atggtagaag cttccattaa accgttagct
    71641 tttactaact tactacccgt ggttaattcg ccgagaatac cgtatttcgc cggtgaatag
    71701 gctgcagcac caacacccac cagcgtatag ccgagaaacg gattgatacc aaagcagata
    71761 ctggctgcgc ccagcagctt caggccgttg gcaaacatca tcacccggcc tttggcgaag
    71821 ctatccgcca cctgcccgac aaacggcgca aaaagaatgt aagcacctac aaacaccatt
    71881 tgcaggatgg gctggctcca ctccggatag aactgcgctt tcagtaacgc cagagtggca
    71941 aacagtaggg cattatcgcc aaacgcagag agaaactgcg ccacgataac cgctttcatc
    72001 cccttcgacc acaacgaagt gttagtgtgc actgactcac tcatcgtgtt gttccacttc
    72061 gtctacccag cttttcaacg tgacaaagtc aggtttgccg ctgccaagta atggcatctg
    72121 tttcaaatag cgaatatcac gcggtacagc aagctccggc acgccgtgct cacgggcata
    72181 ctgttgcaac ttatcgcgcg tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc
    72241 tttgctggca tcgctcttaa tcgcagtggc atggacttta tctggcgaaa caccaagtgc
    72301 cagttgttcc accatttcca gcgacaccat ttcgcctgca attttggcaa agcgttttgc
    72361 gcggccctga atctgcacaa agccctgctc gtcaaaacgc acaatatcgc cagtgtcata
    72421 ccagccgcgc tccatttcgc cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg
    72481 cttctccacc cgcagatagc cgttcattat gttcggccct ttcagttgca ggcgtccgcc
    72541 ctcttcgata ccagggaccg acaacaggcg cgcatccatt cctggtagaa tacgccctac
    72601 cgtaccgggt ttcgccgcca tcggtacgtt gatagaaacg acaggcgcgc attcggtcac
    72661 accgtagcct tcaaggatgc gcaggccaaa tttatcctgc caaagctgtt tggtactttc
    72721 ttgtagtttt tctgcgcctg ccaccacata gcgtagacga tagaagtcat acgggttggc
    72781 gaaacgcgcg tagtgaccga ggaaagtcga agtgccgaac aacacggtgc aactgcggtc
    72841 atacaccaac tccggcacaa tacggtaatg cagcgggctt ggataaagaa acacttctgc
    72901 acctgtgagc agtggcgtaa acaggcctac cgtcagccca aaggagtgaa acagcggtaa
    72961 cgccgacata aagcgatcgt tggtggtgaa gtcggcaatc gttttaatct gctcgacatt
    73021 cgccagaatg cttttatggc tatggacgac gccttttgga tggccttcag aaccggaggt
    73081 aaaaaggatc agcgcctctt cttccggctg ctgtttaacc tgtgccagac gcggcatcag
    73141 caaatgagcg aagatccata ctttgtcggc agtggtgaca tctgctttta aatcttccag
    73201 atagacccag cgcacctgag taagttgctc cggcagatgc cagagtttgc ctttatcgag
    73261 aaactggcgg gaagtgaaga tggttttgat ttcagccgcc gtaatagcac tggtcagccc
    73321 ttttacccca gcagtgtagt tcatcattgc gggaatgcgg cgacgggcga tggccccaaa
    73381 aatcactgcc gcactgatgc ctgcattggg cagcattaag ccgatgcgtt cgccttcaac
    73441 actgtatttt tcaaggatgc gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga
    73501 gtctggggta aaattgacgt cttcgacaca tttcttccct gctccgaagc ggtacattgc
    73561 actcagtaaa gattcgtaca gcgtttcacg cgggcgcacc gccattcgcg cttccatcat
    73621 tatttgatgc agcatttcgc cagcgatttt gcgacggtca cgggcacgcg gcgcatccgg
    73681 catctccacc tgcgttggtg gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt
    73741 aaccagacct ttcaggcggc tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat
    73801 aaccgttgca ccagacttcg ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga
    73861 gcctgtggtg gtgatgcgtc cttcagggaa aatcaccact ggtcggccct gttccaccag
    73921 acgtaccaga tgtttaatcg ccataggttg cgtcgggtcg agaggaacaa agtcgataaa
    73981 tgatttcagc caacgcatat accactgttg gcttattgag gtgtaaacgg caaacactgg
    74041 acgcacaggt aaaaacagtc caagcaaaat gccatcaata aaagagacgt gattaggcgt
    74101 aattagaacg cgctcgccct tcagtgcctg ggggtcaccc gtaacgcgaa cgcgatacaa
    74161 aacacggcac aaatttcgaa aaaagctaaa aagcattcca actccctttg ctctgattca
    74221 gtaaaagcga atggagggag attacacgag ataaagaacg cgagcgacag taaattaggt
    74281 gcgaaaaaaa acctgcgcat ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta
    74341 ccgaataatc tcaccaatca atacctctgg gatcttgatt gtggtctgca cgacgactct
    74401 tcgccagcga gaaaacgcaa aggaatgaag ggaaatgcaa cgaggtgtgt aaattgtcgg
    74461 ttactgttac agattgatga ccggcaaaaa aaaacctgcg catctgcgca ggctggtgta
    74521 attcatgtgc tcaacccgaa gttgacttca cctatcaata cctctgggat caccacttta
    74581 gcaacctgaa gccaaacgcc accagcggtc aatcgcaaca gcgtttcgca aagtgtaacc
    74641 aaaggtttgc aatctctttt tttgtcttac ttatttgcgt ttggctcaca ttcccacgat
    74701 gaaaacacgc cgccccttga accaacgggc gttttccgta acactgaaac aatgtaagcg
    74761 tttacccact aaggtatttt catggcgacc ataaaggatg tagcccgact ggcaggcgtt
    74821 tcagtcgcca ccgtttcccg cgtcattaat aattcaccca aagccagcga agcttcccgg
    74881 ctggctgtgc atagtgcaat ggagtctctt agctatcacc cgaacgccaa cgcccgtgcg
    74941 ctggcgcagc agaccactga aacggtcggt ctggtcgttg gtgatgtttc cgatccgttt
    75001 ttcggtgcaa tggtgaaagc ggtcgaacag gtggcttatc acaccggtaa tttcttattg
    75061 attggcaatg gttaccacaa cgaacaaaaa gagcgtcagg ccattgagca actgatccgc
    75121 catcgctgtg ctgcgttggt cgtccatgcc aaaatgatcc cggatgccga tttagcctca
    75181 ttattgaaac aaatgcccgg tatggtgctg atcaaccgta tcctgcctgg ctttgaaaac
    75241 cgttgtattg ctctggacga tcgttacggt gcctggctgg ctacgcgtca tttaattcag
    75301 caaggtcata cccgcattgg ttatctgtgc tctaaccact ctatttctga cgccgaagat
    75361 cgtctgcaag ggtattacga tgcccttgct gaaagtggta ttgcggccaa tgaccggctg
    75421 gtgacatttg gcgaaccaga cgaaagcggc ggcgaacagg caatgaccga gcttttggga
    75481 cgaggaagaa atttcactgc ggtagcctgt tataacgatt caatggcggc gggtgcgatg
    75541 ggcgttctca atgataatgg tattgatgta ccgggtgaga tttcgttaat tggctttgat
    75601 gatgtgctgg tgtcacgcta tgtgcgtccg cgcctgacca ccgtgcgtta cccaatcgtg
    75661 acgatggcga cccaggctgc cgaactggct ttggcgctgg cggataatcg ccctctcccg
    75721 gaaatcacta atgtctttag tccgacgctg gtacgtcgtc attcagtgtc aactccgtcg
    75781 ctggaggcaa gtcatcatgc aaccagcgac taaccgcagt taaagcaatt ccagcgccag
    75841 taattcttcg atggtctggc gacggcgaat caaccgcgcc tgaccattat caaacagaac
    75901 ttctggtaac agcggacggc tattgtagtt ggatgacatt gatgcgccat atgcccctgt
    75961 atcatgcagt accagataat cacctgcctt cacttccggc aaggcgcggg gttcaacatt
    76021 tcccccttcc tgctgggtaa agacatcgcc cgattcacat aacggtccgg cgacgacggt
    76081 ttccaccgtt ggtgcgtgtt ccagagaacg accatcagct gccagggcac tgatatggtg
    76141 gtaactaccg tacattgccg ggcgcatcag atcgttaaac ccggcatcaa ccagcacaaa
    76201 gtggcggcta cccatttgtt tgacgctccg cacctgagta attaacacgc cagactgcgc
    76261 taccaggaag cgacccggtt caatttccag tttcacaggg tggcccaaat ggcgggcgat
    76321 ttgctcacgc gcggcattcc acagaccata ataatgttcg gtatcaaccg cctcttcacc
    76381 ctgttgataa ggaatagaaa gcccaccgcc cgcagaaata gcctgtaaat cctgaccgaa
    76441 ttcgatgacc tgacgcacca tagcaccaca cacctgttcc agatgggcat aatcaacgcc
    76501 agaaccaatg tgcatgtgaa tgccgaccag ctgcagatga tgacgttgta tcacgtccag
    76561 tgcggcgggc agatcggtgt accagatacc gtgcttgctg ttttcgccac cggtattggt
    76621 tttttggcta tgtccgtgac caaaccccgg attaacgcgc agccataccc gatgccctgg
    76681 cgaaacctgg cccagttggt cgaacatatc aacagaaccc gcattcaccg gaatttgcaa
    76741 ttcactgacg cgttcaagcg tcgcctgatc gataacatct gccgtaaaaa caatatcatc
    76801 ggggtgcgtt tgcggattgt aacccgccgc caacgcacgc tctatttcgc ctaacgagac
    76861 ggaatccact ttcacgccct gctcacgcat taagcgcaaa atatgaatat tggaacaggc
    76921 tttctgtgca aagcgcacca catcaaactg tttcagcgct gcaatctgcc gacgaataat
    76981 ttgcgcatcg tagacccaca ccgggcagcc aaattcagcg ggcaaacgca gcagattttc
    77041 ggcggtgaga tcggtatcgg tgctgaacag tgaatgtggc ataacaaact ccagatgagt
    77101 gcttttttat gattacgcca catcataaaa agaataaaaa atatcgattt atgtcgagtc
    77161 tatgcaaaaa tgatatggat taccggattg cgagagagcg ctaatggccg ccgttaactt
    77221 acgtcatatt gaaatttttc atgcggtaat gaccgccgga agcctgactg aggcagcaca
    77281 cctgctacac acctcacagc caaccgtcag ccgcgaactg gcgcgctttg agaaggtgat
    77341 cgggctgaaa ttgtttgagc gcgtacgtgg acgattacat cctaccgtgc aaggactgcg
    77401 tctgtttgaa gaagtgcaac gatcctggta cggactggat cgcattgtca gcgccgcaga
    77461 aagtctgcgc gagtttcgcc agggagaact gtctattgcc tgcctgccgg tcttttcgca
    77521 atctttttta ccgcagctcc tgcaaccctt tctggtacgt tatcccgatg tcagcttaaa
    77581 tatcgtgccc caggaatcac cgctacttga agagtggctc tcggcccagc gtcatgattt
    77641 aggactcact gagacactcc atacgcctgc gggaacagaa cgtaccgaat tactctcttt
    77701 agatgaattg tgtgtgttac ctccgggtca tccgctggcg gtaaaaaagg tattaacgcc
    77761 ggatgatttt cagggtgaga actacatcag cctttcccgt actgacagct atcgccagtt
    77821 gctggatcag ctatttactg aacatcaggt taaacgacgc atgatcgtag aaacccacag
    77881 cgccgcgtca gtctgcgcaa tggtacgggc gggggtaggt gtttcggtgg ttaacccgct
    77941 caccgcactg gattatgcgg caagcggttt agtggtgcgg cggttcagta ttgcggttcc
    78001 gttcaccgtc agcctgatcc gccccctgca ccgcccgtca tcagcgctgg tgcaggcgtt
    78061 tagtgagcat ttacaagcgg ggttaccgaa actggtcact tctcttgacg ctattttgtc
    78121 gtcagctacg acagcttaaa agcgacagca tcctcggcat ggatcgccgc agtatcaaac
    78181 acaggcaaaa cactgcgctc ttctggcacc agtaaaccaa tttctgtgca gccaaaaatg
    78241 acgccctgtg cgccctgttc tgcaaggcga gcaatcactt gcgcataata agcgcgtgac
    78301 gcttcggtaa attgccccag acacagttct tcaaaaataa tctgattaat tttcgcccgt
    78361 tcatccgctt caggaataag acaattgatg gaaaattgtt ccgtcagccg cccgcgataa
    78421 aaatcctgtt ccatggtgta acgcgtaccc agcagcgcca cacgagtcat tccggccccg
    78481 gtaattgcac gtccggtggc atccgcaatg tgtaagaaag gcagagtgca acgtgactca
    78541 atggcatccg ccactttatg catcgtattg gtacataaca caataccttc tgcgcccgcc
    78601 cgctgtaagc caagcgccgc ctcagccaga atgtccccgg ttttatccca ttccccgcga
    78661 cgctggcact cttctatttc atgaaaatcg acgctatgta gcagcacttg cgcagagtga
    78721 agcccaccaa gccgctgttt aatgccttca tttatcagac gatagtaagg aatggtggat
    78781 tcccagctca ttcctcccag caaaccaatt gttttcattc ctctctcctg atgtgtgtta
    78841 ccgcaacaag gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccgatctct
    78901 attaacgaaa aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat
    78961 ttctcaactt ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg
    79021 tttccggaat gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc
    79081 agaacgtacc ggcagcgcca atgctatcaa gcagtgtcag gaaggtcgcg ccgataatca
    79141 tattcgacac ccagtttgtc gtcgtcgaac aggtaatacc gaaatcgcgg catttcagcg
    79201 gctgaatttc agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa
    79261 tacacatcat cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat
    79321 caaactgcat caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa
    79381 ttttcagagc cggtttacga cctgctttat ctaccgtaaa gaccgcaatg aaggtcgcga
    79441 acataaaggt cagtcccacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc
    79501 ccgccatttt gaagatacgc ggcgcatagt acatgatgat gttcataccg gtaaactgct
    79561 gcatcgcctg caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa
    79621 acagtgccca accgccttgt tttaacttca ggctttcacg aatttcgttg agttcttctc
    79681 gcgctttttc cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc
    79741 ccttttccgc cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga
    79801 ctgctggtaa agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg
    79861 tatcggataa aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca
    79921 tcttaccgcg aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg
    79981 cgatcccgac agcaatgccc agcaccacac gagcggcgat taacatctct acgctggtcg
    80041 caaaagcgga ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt
    80101 tacgccccag gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca
    80161 tcatgctact aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga
    80221 acggcaacgc tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag
    80281 cagctaccga aacaaacata ttcatacgcc gcgtatcacg caaagaacgt ggcgttaaag
    80341 cagattccgt attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa
    80401 aaatacgtga acaattcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt
    80461 gctgcgacat gccgttatgt gatggatatt ccaattttaa aattaagttg aattattgag
    80521 attattatta accacctaat tttaaagcag ataaaattca taaagttcat taattgataa
    80581 ttgatatgga ttatttcata accatgatat ggattatgat gatctagaga tataaaaaac
    80641 cctgccatgc ggcagggtca taaaagtaag aggaatgaat taacgcgcca gccaaccgcc
    80701 atccacagca atggtataac catttacata atctgaagcg ctggaggcaa gaaacactac
    80761 cggccccatc aggtcactcg gcagtcccca acggccagct ggaatgcggt cgagaatttc
    80821 cgcgctacgt tgttcatctg cccgcagttg ttgggtattg ttggtcgcca tgtaacccgg
    80881 agctatcgca ttaacattaa tgttgtgttt agcccattca ttcgccatca atcgcgtcac
    80941 gcccatcacg ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga
    81001 gagcattgac gcgatattga taatcttgcc gccattgcct tgcgcgatga agtgtttcgc
    81061 cgctgcctga gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc
    81121 cttttcgctg aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat
    81181 atcaatatga ccaaactcag ctaccgcgcg atccagcagt ccaggaatac catcaatctt
    81241 tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gcttgatggt
    81301 ttcagtcggt tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa
    81361 cgccatcccc tggcccagcc cagtatcaca accagtgacg accgcaactt taccttcgag
    81421 agaaaatgca cttaaaatca taacaatacc ttattcgttt atgcccacaa ctagcgcaaa
    81481 tctttaacgg ccacatggtc catatcatca aagacctggt tttcaccgac catgccccag
    81541 ataaaggtat aagctttggt tccgacgccg gaatgaatcg accagctcgg ggagatcacc
    81601 gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat catgtggaaa
    81661 acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg ctcgtgggtg
    81721 tgacacggca tggtgttcca caagttaccc ggagccagct ccgtcagccc catactcaat
    81781 tggcaggttt ccagcacatc cgggacaaaa tatttgttaa tcgtgcgacg gttactggtg
    81841 aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt ggtaggatac
    81901 gtcgtatgcg cgggtgcgca attgtaataa aacttcgccg gagtgccggt atcaatacta
    81961 gcaaagacaa cttcttttgc gcctttaccc acatacaggg cgtcgcggtg accgatttca
    82021 tagcattgac catcgacagt aatcgtaccg gcaccgccaa tattgataac acctaactcg
    82081 cgacgttcaa ggaaataact tacgccgagt tgtttaccca cttccccgcc aacggaaacc
    82141 gttttagtta ccggcataat gccgccaata ataatacggt caatgtggct gtaaaccatg
    82201 gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag cccctgggtt
    82261 tccagcgttt ttgcgtgcgc actgtggatg ctctgtctta cgtccacatc aacctccgat
    82321 aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt tctctataag
    82381 gggatgatat gcccatggga tcgggttttc aattaaaaat aaaacaatgt ttcattttta
    82441 agttaaggat taaaaaaagt gcagtgtcga tcacgaatct gtcatgagaa tggaaaaatc
    82501 agggagcccg gaaaatgtat gtcgacttgc cagccatcat tattcaacag attaatgacg
    82561 gcagatattt atcggtaagg aggaaatgca gagctattcg tcacgttcaa tggtcaatgc
    82621 cacaccctgg cccccgccga tacaaagcgt tgccagtcct ttgcgggcat tacgtttcac
    82681 catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag
    82741 tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca tcttgccaac
    82801 cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata agatcaacat cagccaactg
    82861 ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc
    82921 cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta aattcaacgc
    82981 tcgtgctttg gcttcgctca tcatcattac cgcagctgcg ccatcgttta tggatgatgc
    83041 attacccgct gtcacagaac cgagactatc aaatgaagga tttaaacggg ctaaaccttc
    83101 tgcactggcg tcagtgtgtg gctgttcatc ggtatcaaca accaacgtct gaccgttact
    83161 ttgggtcatt accgggacga tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc
    83221 tttttgttgc gagctaagtg cgtaagcatc ctgcaattga cggctgatgc catattcgcg
    83281 agccagattt tcggcggtga caccaatatg ataatcattg aaggcatccc ataacccatc
    83341 atgcacaaga ctgtcaacca gctggctatt gccaagctgt gcaccggtgc ggctatcagt
    83401 cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg gcgatgacaa tatcagcctc
    83461 gccacactgt atcgcctgag tagccagatg cagtgcttta agcccggaac cgcagacgtc
    83521 attaatcgtg attgcagaaa cgctattagg cagaccacct ttaatagccg attgccttgc
    83581 cggattctgc cctgcccctg cagtcaacac ctgaccaaga attacttcat ccaccgcata
    83641 tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc agactaccaa gttccacggc
    83701 ggaatgaccc gctaacgcac cgcgaaagca gccgataggt gtccgtaacg ccccgacaat
    83761 cacaacgtct ttcatcacaa cctcgcacca attaacagtg caatagtaaa tgattgttaa
    83821 ttacagttat cttaattgtt taaaaaaagt gatttttatc acaaaggaaa tatacctgag
    83881 cagtagtcag agtcataact ggcacgtaag gtttgcaacc actaccccac caatagaggg
    83941 gtagataggg cgttaatctc ccatacttaa cctggtttat ggtaaattgc cctccatttt
    84001 gtttaatttg tagatgatac gttcagataa tgtctaatat ttggtctaaa gaagaaactc
    84061 tgtggagttt cgcgctctac ggcacagccg ttggtgcagg cacgctcttc cttcctattc
    84121 agttaggttc ggcaggggct gtggtcctgt ttattactgc tctggtcgcc tggcctttaa
    84181 catactggcc acataaagct ttatgccagt tcatcctctc atcgaaaaca tcagcaggtg
    84241 aagggataac gggcgcggta acacactact atggcaagaa gattggtaat ctgattacca
    84301 cgctgtactt catcgccttt tttgtcgtcg tgttgatata tgcagtggca attaccaact
    84361 cacttacgga acagctggca aagcatatgg ttattgatct tcgcatccgt atgttggtga
    84421 gtctgggtgt tgtattaatt ctgaatctca tttttctgat gggacgtcat gccactattc
    84481 gggtaatggg atttttggta ttcccattga ttgcctattt cttatttctt tccatttacc
    84541 ttgtaggtag ttggcaacct gatctattaa caacccaggt agagttcaat cagaataccc
    84601 ttcaccagat atggatatcg attcccgtga tggttttcgc ctttagccat acgcccatta
    84661 tttctacgtt tgccatagac agacgtgaaa aatatggcga acacgctatg gataaatgca
    84721 aaaaaattat gaaagtcgct tatctcatca tctgcataag tgtactgttc tttgtcttta
    84781 gctgcctgct ttctattcca ccttcgtata ttgaagcggc taaagaagaa ggggtcacca
    84841 ttttatcggc gctttctatg ctgccgaacg ccccagcatg gttgtcaatt tccgggatta
    84901 ttgtcgcagt agttgcgatg tcgaaatcat tcctgggtac gtactttggc gttattgaag
    84961 gtgccacgga ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa agtcgtgcat
    85021 ttaaccgcgc actatcaatt atgttggtat cgctgattac cttcattgtt tgttgcatta
    85081 acccgaacgc gatttcgatg atttatgcga tcagcggccc gctcattgcc atgatacttt
    85141 tcatcatgcc tacgctatca acatatctca tcccggcgct taaaccctgg cgctccatcg
    85201 gaaatctgat tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc tttagctaat
    85261 atccattgtg aatggttcaa aattgctaca attttgaacc attccagttt tcataatgtg
    85321 catttgttat tattccgctc tatcacttct ctcgtcatat cctagtatta tcagttagtt
    85381 aaacacacaa agagatctga acatcttaaa taagactatt taagatgcat aacttagatt
    85441 cgcaagatat cttctggtca ttcaaaaaca atttgcgata tagatattct cataccatga
    85501 gatagttatc tgaaccaccc tcaaccaggt aagattaaat cagtgaaaca taatcatatt
    85561 taataacgag aaatgcattt taaaatgtct taatcagaat tatagcaaat acagattaat
    85621 cgcaattttt cccagcaaaa ttacaaaatc atggtgtatt gacatcatca atcaccaatg
    85681 aaattatgca attatatacg gatagggagg ttcttaacat ggggctttgt agtcgttata
    85741 aaagtcttac atgcaatagt tgctcaatgc attgccaaat aatgccagaa gagtcaccgc
    85801 gtttgcagta ctgcgcaaac tcgtgttttt gtatgtggac cgaagaaagt tcatatttta
    85861 atcgtggtgt ggtagaaggt atcttaacaa aaaaccacaa tgcgagatta agcggatata
    85921 tttttgtcga tttttcagta agttttttac gtctattcct tgaaaaagac tggattgact
    85981 atcttgcaag tactgacatg gggattgttt tagtcagcga ccgcaatatg caatcgttag
    86041 ctaattactg gcgaaaacac aactctgcaa tatctgctgt catatataat gatgatggtc
    86101 ttgatgttgc caatgaaaag atcagacaac tatttattgg tcgttattta tcatttacca
    86161 gaggtaacac attaactcag atggaattca ccataatggg gtatatggtt tctggttata
    86221 atccatatca gattgctgaa gttctggata tggatatccg tagcatctac gcgtacaagc
    86281 aacgaatcga aaagagaatg ggtggtaaaa taaacgaatt atttattcgt tcacattcgg
    86341 tccaacattg atactcaaca atctgccaga atcatactct gtttatacgg gcatttattg
    86401 aaatgcccgt attttataaa taggccgtcc cctcaagagt aaacaccata gtatttaaaa
    86461 ccatcacacg aaaaattcag aagc
//
LOCUS       DAZFBR010000007        81604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFBR010000007 DAZFBR010000000
VERSION     DAZFBR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.007"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(165..1115)
                     /gene="dusC"
                     /locus_tag="ACHJZ2_000590"
     CDS             complement(165..1115)
                     /gene="dusC"
                     /locus_tag="ACHJZ2_000590"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO4566760.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
     gene            complement(1275..2468)
                     /locus_tag="ACHJZ2_000591"
     CDS             complement(1275..2468)
                     /locus_tag="ACHJZ2_000591"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO4566761.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(2483..3127)
                     /gene="maiA"
                     /locus_tag="ACHJZ2_000592"
     CDS             complement(2483..3127)
                     /gene="maiA"
                     /locus_tag="ACHJZ2_000592"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO4566762.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(3136..3837)
                     /locus_tag="ACHJZ2_000593"
     CDS             complement(3136..3837)
                     /locus_tag="ACHJZ2_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO4566763.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(3852..4880)
                     /gene="gtdA"
                     /locus_tag="ACHJZ2_000594"
     CDS             complement(3852..4880)
                     /gene="gtdA"
                     /locus_tag="ACHJZ2_000594"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO4566764.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(4892..6250)
                     /locus_tag="ACHJZ2_000595"
     CDS             complement(4892..6250)
                     /locus_tag="ACHJZ2_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4566765.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            6377..7285
                     /locus_tag="ACHJZ2_000596"
     CDS             6377..7285
                     /locus_tag="ACHJZ2_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4566766.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            7416..7814
                     /gene="yohJ"
                     /locus_tag="ACHJZ2_000597"
     CDS             7416..7814
                     /gene="yohJ"
                     /locus_tag="ACHJZ2_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO4566767.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            7811..8506
                     /gene="yohK"
                     /locus_tag="ACHJZ2_000598"
     CDS             7811..8506
                     /gene="yohK"
                     /locus_tag="ACHJZ2_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO4566768.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            8636..9520
                     /gene="cdd"
                     /locus_tag="ACHJZ2_000599"
     CDS             8636..9520
                     /gene="cdd"
                     /locus_tag="ACHJZ2_000599"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO4566769.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            9670..10389
                     /gene="sanA"
                     /locus_tag="ACHJZ2_000600"
     CDS             9670..10389
                     /gene="sanA"
                     /locus_tag="ACHJZ2_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO4566770.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            10392..10631
                     /gene="yeiS"
                     /locus_tag="ACHJZ2_000601"
     CDS             10392..10631
                     /gene="yeiS"
                     /locus_tag="ACHJZ2_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO4566771.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            10825..12063
                     /gene="preT"
                     /locus_tag="ACHJZ2_000602"
     CDS             10825..12063
                     /gene="preT"
                     /locus_tag="ACHJZ2_000602"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO4566772.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            12057..13292
                     /gene="preA"
                     /locus_tag="ACHJZ2_000603"
     CDS             12057..13292
                     /gene="preA"
                     /locus_tag="ACHJZ2_000603"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO4566773.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(13535..14545)
                     /gene="mglC"
                     /locus_tag="ACHJZ2_000604"
     CDS             complement(13535..14545)
                     /gene="mglC"
                     /locus_tag="ACHJZ2_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO4566774.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(14561..16081)
                     /gene="mglA"
                     /locus_tag="ACHJZ2_000605"
     CDS             complement(14561..16081)
                     /gene="mglA"
                     /locus_tag="ACHJZ2_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO4566775.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(16142..17140)
                     /gene="mglB"
                     /locus_tag="ACHJZ2_000606"
     CDS             complement(16142..17140)
                     /gene="mglB"
                     /locus_tag="ACHJZ2_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO4566776.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(17419..18459)
                     /gene="galS"
                     /locus_tag="ACHJZ2_000607"
     CDS             complement(17419..18459)
                     /gene="galS"
                     /locus_tag="ACHJZ2_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO4566777.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(18601..19758)
                     /gene="yeiB"
                     /locus_tag="ACHJZ2_000608"
     CDS             complement(18601..19758)
                     /gene="yeiB"
                     /locus_tag="ACHJZ2_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO4566778.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(19775..20443)
                     /gene="folE"
                     /locus_tag="ACHJZ2_000609"
     CDS             complement(19775..20443)
                     /gene="folE"
                     /locus_tag="ACHJZ2_000609"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO4566779.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            20701..21537
                     /gene="yeiG"
                     /locus_tag="ACHJZ2_000610"
     CDS             20701..21537
                     /gene="yeiG"
                     /locus_tag="ACHJZ2_000610"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4566780.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(21569..23548)
                     /gene="cirA"
                     /locus_tag="ACHJZ2_000611"
     CDS             complement(21569..23548)
                     /gene="cirA"
                     /locus_tag="ACHJZ2_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO4566781.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(23840..25309)
                     /gene="lysP"
                     /locus_tag="ACHJZ2_000612"
     CDS             complement(23840..25309)
                     /gene="lysP"
                     /locus_tag="ACHJZ2_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO4566782.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(25514..26395)
                     /gene="yieE"
                     /locus_tag="ACHJZ2_000613"
     CDS             complement(25514..26395)
                     /gene="yieE"
                     /locus_tag="ACHJZ2_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO4566783.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            26494..27543
                     /gene="yeiH"
                     /locus_tag="ACHJZ2_000614"
     CDS             26494..27543
                     /gene="yeiH"
                     /locus_tag="ACHJZ2_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO4566784.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            27617..28474
                     /gene="nfo"
                     /locus_tag="ACHJZ2_000615"
     CDS             27617..28474
                     /gene="nfo"
                     /locus_tag="ACHJZ2_000615"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO4566785.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            28477..29565
                     /gene="yeiI"
                     /locus_tag="ACHJZ2_000616"
     CDS             28477..29565
                     /gene="yeiI"
                     /locus_tag="ACHJZ2_000616"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4566786.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(29621..30871)
                     /gene="nupX"
                     /locus_tag="ACHJZ2_000617"
     CDS             complement(29621..30871)
                     /gene="nupX"
                     /locus_tag="ACHJZ2_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4566787.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(30971..31912)
                     /gene="rihB"
                     /locus_tag="ACHJZ2_000618"
     CDS             complement(30971..31912)
                     /gene="rihB"
                     /locus_tag="ACHJZ2_000618"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO4566788.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLALTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            32042..32740
                     /gene="yeiL"
                     /locus_tag="ACHJZ2_000619"
     CDS             32042..32740
                     /gene="yeiL"
                     /locus_tag="ACHJZ2_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO4566789.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(32811..34061)
                     /locus_tag="ACHJZ2_000620"
     CDS             complement(32811..34061)
                     /locus_tag="ACHJZ2_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4566790.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(34155..35093)
                     /gene="psuG"
                     /locus_tag="ACHJZ2_000621"
     CDS             complement(34155..35093)
                     /gene="psuG"
                     /locus_tag="ACHJZ2_000621"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO4566791.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(35081..36022)
                     /gene="psuK"
                     /locus_tag="ACHJZ2_000622"
     CDS             complement(35081..36022)
                     /gene="psuK"
                     /locus_tag="ACHJZ2_000622"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO4566792.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(36446..38137)
                     /gene="fruA"
                     /locus_tag="ACHJZ2_000623"
     CDS             complement(36446..38137)
                     /gene="fruA"
                     /locus_tag="ACHJZ2_000623"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO4566793.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(38154..39092)
                     /gene="fruK"
                     /locus_tag="ACHJZ2_000624"
     CDS             complement(38154..39092)
                     /gene="fruK"
                     /locus_tag="ACHJZ2_000624"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO4566794.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(39092..40222)
                     /gene="fruB"
                     /locus_tag="ACHJZ2_000625"
     CDS             complement(39092..40222)
                     /gene="fruB"
                     /locus_tag="ACHJZ2_000625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO4566795.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            40590..41771
                     /gene="setB"
                     /locus_tag="ACHJZ2_000626"
     CDS             40590..41771
                     /gene="setB"
                     /locus_tag="ACHJZ2_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO4566796.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(41768..42022)
                     /gene="yeiW"
                     /locus_tag="ACHJZ2_000627"
     CDS             complement(41768..42022)
                     /gene="yeiW"
                     /locus_tag="ACHJZ2_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4566797.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            42177..42749
                     /gene="yeiP"
                     /locus_tag="ACHJZ2_000628"
     CDS             42177..42749
                     /gene="yeiP"
                     /locus_tag="ACHJZ2_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO4566798.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            42972..44438
                     /gene="yeiQ"
                     /locus_tag="ACHJZ2_000629"
     CDS             42972..44438
                     /gene="yeiQ"
                     /locus_tag="ACHJZ2_000629"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO4566799.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQPPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            44556..45542
                     /gene="yeiR"
                     /locus_tag="ACHJZ2_000630"
     CDS             44556..45542
                     /gene="yeiR"
                     /locus_tag="ACHJZ2_000630"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO4566800.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            45581..46294
                     /gene="lpxT"
                     /locus_tag="ACHJZ2_000631"
     CDS             45581..46294
                     /gene="lpxT"
                     /locus_tag="ACHJZ2_000631"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO4566801.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            46707..47273
                     /gene="mepS"
                     /locus_tag="ACHJZ2_000632"
     CDS             46707..47273
                     /gene="mepS"
                     /locus_tag="ACHJZ2_000632"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO4566802.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            47454..49010
                     /gene="pdeN"
                     /locus_tag="ACHJZ2_000633"
     CDS             47454..49010
                     /gene="pdeN"
                     /locus_tag="ACHJZ2_000633"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4566803.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            49092..50906
                     /gene="yejA"
                     /locus_tag="ACHJZ2_000634"
     CDS             49092..50906
                     /gene="yejA"
                     /locus_tag="ACHJZ2_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO4566804.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMQWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            50907..52001
                     /gene="yejB"
                     /locus_tag="ACHJZ2_000635"
     CDS             50907..52001
                     /gene="yejB"
                     /locus_tag="ACHJZ2_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO4566805.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            52001..53026
                     /gene="yejE"
                     /locus_tag="ACHJZ2_000636"
     CDS             52001..53026
                     /gene="yejE"
                     /locus_tag="ACHJZ2_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4566806.1"
                     /translation="MSRLNPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            53028..54617
                     /gene="yejF"
                     /locus_tag="ACHJZ2_000637"
     CDS             53028..54617
                     /gene="yejF"
                     /locus_tag="ACHJZ2_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO4566807.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(54621..54965)
                     /gene="yejG"
                     /locus_tag="ACHJZ2_000638"
     CDS             complement(54621..54965)
                     /gene="yejG"
                     /locus_tag="ACHJZ2_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO4566808.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(55298..56488)
                     /gene="bcr"
                     /locus_tag="ACHJZ2_000639"
     CDS             complement(55298..56488)
                     /gene="bcr"
                     /locus_tag="ACHJZ2_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO4566809.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(56516..57211)
                     /gene="rsuA"
                     /locus_tag="ACHJZ2_000640"
     CDS             complement(56516..57211)
                     /gene="rsuA"
                     /locus_tag="ACHJZ2_000640"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO4566810.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            57360..59120
                     /gene="radD"
                     /locus_tag="ACHJZ2_000641"
     CDS             57360..59120
                     /gene="radD"
                     /locus_tag="ACHJZ2_000641"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4566811.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            59245..59529
                     /gene="rplY"
                     /locus_tag="ACHJZ2_000642"
     CDS             59245..59529
                     /gene="rplY"
                     /locus_tag="ACHJZ2_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO4566812.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNIQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(59668..60675)
                     /gene="yejK"
                     /locus_tag="ACHJZ2_000643"
     CDS             complement(59668..60675)
                     /gene="yejK"
                     /locus_tag="ACHJZ2_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO4566813.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDFTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            60857..61084
                     /gene="yejL"
                     /locus_tag="ACHJZ2_000644"
     CDS             60857..61084
                     /gene="yejL"
                     /locus_tag="ACHJZ2_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO4566814.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            61104..62864
                     /gene="yejM"
                     /locus_tag="ACHJZ2_000645"
     CDS             61104..62864
                     /gene="yejM"
                     /locus_tag="ACHJZ2_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO4566815.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            62939..63015
                     /locus_tag="ACHJZ2_000646"
     tRNA            62939..63015
                     /locus_tag="ACHJZ2_000646"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62973..62975,aa:Pro,seq:ggg)
     gene            complement(63116..65707)
                     /gene="yejO"
                     /locus_tag="ACHJZ2_000647"
     CDS             complement(63116..65707)
                     /gene="yejO"
                     /locus_tag="ACHJZ2_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFO4566816.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDESVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            66027..66674
                     /gene="narP"
                     /locus_tag="ACHJZ2_000648"
     CDS             66027..66674
                     /gene="narP"
                     /locus_tag="ACHJZ2_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO4566817.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(66709..67761)
                     /gene="ccmH"
                     /locus_tag="ACHJZ2_000649"
     CDS             complement(66709..67761)
                     /gene="ccmH"
                     /locus_tag="ACHJZ2_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO4566818.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(67758..68315)
                     /gene="dsbE"
                     /locus_tag="ACHJZ2_000650"
     CDS             complement(67758..68315)
                     /gene="dsbE"
                     /locus_tag="ACHJZ2_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO4566819.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(68312..70255)
                     /gene="ccmF"
                     /locus_tag="ACHJZ2_000651"
     CDS             complement(68312..70255)
                     /gene="ccmF"
                     /locus_tag="ACHJZ2_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO4566820.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(70252..70731)
                     /gene="ccmE"
                     /locus_tag="ACHJZ2_000652"
     CDS             complement(70252..70731)
                     /gene="ccmE"
                     /locus_tag="ACHJZ2_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO4566821.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(70728..70937)
                     /gene="ccmD"
                     /locus_tag="ACHJZ2_000653"
     CDS             complement(70728..70937)
                     /gene="ccmD"
                     /locus_tag="ACHJZ2_000653"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO4566822.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(70934..71671)
                     /gene="ccmC"
                     /locus_tag="ACHJZ2_000654"
     CDS             complement(70934..71671)
                     /gene="ccmC"
                     /locus_tag="ACHJZ2_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO4566823.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(71713..72375)
                     /gene="ccmB"
                     /locus_tag="ACHJZ2_000655"
     CDS             complement(71713..72375)
                     /gene="ccmB"
                     /locus_tag="ACHJZ2_000655"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO4566824.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(72372..72995)
                     /gene="ccmA"
                     /locus_tag="ACHJZ2_000656"
     CDS             complement(72372..72995)
                     /gene="ccmA"
                     /locus_tag="ACHJZ2_000656"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO4566825.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(73008..73610)
                     /gene="napC"
                     /locus_tag="ACHJZ2_000657"
     CDS             complement(73008..73610)
                     /gene="napC"
                     /locus_tag="ACHJZ2_000657"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO4566826.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(73620..74069)
                     /gene="napB"
                     /locus_tag="ACHJZ2_000658"
     CDS             complement(73620..74069)
                     /gene="napB"
                     /locus_tag="ACHJZ2_000658"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO4566827.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(74066..74929)
                     /gene="napH"
                     /locus_tag="ACHJZ2_000659"
     CDS             complement(74066..74929)
                     /gene="napH"
                     /locus_tag="ACHJZ2_000659"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO4566828.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(74916..75611)
                     /gene="napG"
                     /locus_tag="ACHJZ2_000660"
     CDS             complement(74916..75611)
                     /gene="napG"
                     /locus_tag="ACHJZ2_000660"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO4566829.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(75618..78104)
                     /gene="napA"
                     /locus_tag="ACHJZ2_000661"
     CDS             complement(75618..78104)
                     /gene="napA"
                     /locus_tag="ACHJZ2_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO4566830.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(78101..78364)
                     /gene="napD"
                     /locus_tag="ACHJZ2_000662"
     CDS             complement(78101..78364)
                     /gene="napD"
                     /locus_tag="ACHJZ2_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO4566831.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(78354..78848)
                     /gene="napF"
                     /locus_tag="ACHJZ2_000663"
     CDS             complement(78354..78848)
                     /gene="napF"
                     /locus_tag="ACHJZ2_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO4566832.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            78957..79121
                     /gene="yojO"
                     /locus_tag="ACHJZ2_000664"
     CDS             78957..79121
                     /gene="yojO"
                     /locus_tag="ACHJZ2_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO4566833.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLVCSLIRI"
     gene            79255..79743
                     /gene="eco"
                     /locus_tag="ACHJZ2_000665"
     CDS             79255..79743
                     /gene="eco"
                     /locus_tag="ACHJZ2_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO4566834.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(79893..81539)
                     /gene="mqo"
                     /locus_tag="ACHJZ2_000666"
     CDS             complement(79893..81539)
                     /gene="mqo"
                     /locus_tag="ACHJZ2_000666"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO4566835.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT        19879 a        22030 c        20169 g        19526 t
ORIGIN      
        1 tttgttcgcc agagcccggg ggtgcagggg gaggaggcga ttgtcctccc cctgcgcgct
       61 ccttgcgcca gtggcaatat gtggcttagc tcatgaaagg agcgcaacaa gatgatgaag
      121 ctgaataagc gattcattgt ttctgataaa ctgcgattcc actattatcg cagcttaccg
      181 atatcaattg cctgaatagc ccttgcaata tcaggggaat tattcaacac acgaacatgc
      241 tgaaataatt ctgttgcttc atcgtattct ttacgcaaat aactcaacca ctgtttaatc
      301 cgcgctacgt gatataaccc ggtatcgccc tgcttttcca gacgggtata tttttgcagc
      361 aaagcaacca cctccggcca cggcattcgc ggttcgttat attttactac ccggctcagg
      421 ttgggaatat tgagcgcccc gcgaccaatc atcactgagt cgcagccgct gatcgccatg
      481 cattcttgcg cgctctgcca gtcccagatt tcgccgttgg caatcaccgg aatattcagc
      541 cgctggcgaa tctcgccaat cgcctgccag tcaatatgct ccgcgcggta accctgctct
      601 tttgtccgtc catgcaccac cagctccgta gcaccagcct gttgaaccgc atcggcgatt
      661 tcaaatttct tctcaccgct gtcccagccc agacgcactt tcacgctgac gggcaaatgc
      721 gccggtacag cttcacgcat cgcttttgca ccctggtaga tgagttcagg atctttgagt
      781 aacgtcgccc caccaccgct accgttaacc gttttcgacg ggcagccgca attgagatcc
      841 acgccccagg aacctaattc caccgcacgg gcggcgttct ctgccagcca ttgtgggaac
      901 tgacccaaca actgcacgcg caccagcgta ccggatggtg tccggctggc gttttgtagc
      961 tcagggcaaa tgcgatgaaa gactttcacc ggcagcagtt gatccaccac gcggacgaac
     1021 tcggtgatgc acagatcgta gtcgttaact tcggtcagca attcacgcac cagagagtca
     1081 agcactccct ccatcggagc cagtaacaca cgcatatcat cacccgcaaa aaaatgaggc
     1141 gctatgttag cgcctcctgt gtttgcagac aaactgactt actgattaat tcgggcaaga
     1201 tttttaccct caccctaacc ctctcccgaa ggggcgaggg gaccgcccgt gcgcctgatt
     1261 gggtgaagaa gtgcttagcg tggttgcaaa cagttatctt ctttccagcc gtacaaccac
     1321 tccatgccgc gataaaattc tgcctgcgat ttccctttcc acagcagatt acgtacctgg
     1381 cgttctaccc ctgcggcgtg gtaaacccgc cccatttcac gggtcgacca gacgatgcgc
     1441 gcggtgcgcg gaattcgtac cgactcatac agcgcaaagg cttgcgccgc atcaccctca
     1501 cactgcgcca gcgccttacc cagcgttact gcatcttcca gcgccataca ggctccctgc
     1561 gccatatatt gcgccaccgg atgggccgaa tcgcccacaa gcgttatgcg cttcgttccc
     1621 cacttcgcca ccggttcacg gtcagcggtt gaccagcgtc gccatgaagt cggtttatcc
     1681 aacatctggc gcggacgggg gtgaatccct tcaaagtaag agagcacttc ctctttacta
     1741 ccatccttca cgccccactc ttcctgctgg cggctgtgga atgttaccac caggttatac
     1801 tgcttgccgc cgcgcaaagg gtaatgcacc aagtgacaat gcggccccgc ccacagtacc
     1861 ggtgcgttaa tgcgcaaatc ttccggcata tcggcacgat cgataaccgc acggtagaca
     1921 acatgtccgg tgacacgcgg cgcatcgccc aataagctct ggcgcaccac tgacttcacg
     1981 ccgtcacagc caatcagaat atcgccggtc cagctattgc cgcgatcgtc aaacacggtg
     2041 acatcgtctt ccgtctcgcg aatatccacg acgttggtgg aggtgtgata ttccacgtcc
     2101 ggatgcgtca gtaccgcttc ccacaccgag gcgtgaatat caacccggtg gatcaccgcg
     2161 taagggccgc cgaaatggtc acgaaaggcc tgcccggttt caatgcacac cacttcttcg
     2221 gcgttcaccg catccatcat ggtaatgtga tcggtaaaca ccgcgcgctg gcgggcaact
     2281 tcgccgacgc cgaggctgtc cagcgccgaa aacgcatttg gccccaactg gatgcccgcg
     2341 ccaatctcac cgatctcatg cgccttttcc agcagcacca ctttgatccc caggcgggcc
     2401 agtgacagcg cagttgccgc accgccgatc ccgcctccca caattactgc gcgtgtcact
     2461 ttcgtcatta tagttctcct ggtcaggctg aaattttatc ttgttgattt tctggcgctg
     2521 cggcaatgaa cgctggcagc tgtgtgcagg cgtcgtacac cgctttgcag cgtggatacc
     2581 cgctcaaatc gcagttcatg cgtagcgcgt tcgcccactg cgggacaagg cagcaatctg
     2641 ccagcgtcgg cgtctcgccc acacaaaatt gccccgactg gttctggcgc aacagttgtt
     2701 ccaccgcgct taacccttgc tggatccagt gcgcatacca acgttttttt tcttcttcac
     2761 tgacgttcag ttcttcggtc aggtagcgca gcacgcgcaa gttattcaat ggatgaatat
     2821 cacaagcgat ggcatagaca atttccagca ccttgttgcg ggccggctct tcctgcggca
     2881 ccagcggaac ttgcgggtag tgcctctcca gccagtcaat aatcgccagc gattgtccga
     2941 gtgattcgcc ctcgtcagtc agcagcgtcg gcaccagccc gaccggattc atccgccgat
     3001 aggccagttc attttgctga ccaatacgga tattcacccc ttcggtctgg taattaatgc
     3061 ctttcagcgc cagcgcaatg cgtacccggt acgacgctga actattaaaa aaactgtaca
     3121 gcttcatcat gcacctcaga caatcttcac cgcgataggc gttaagccct cgacgttgcc
     3181 tgtgatcaca tcacctttca ctaccgggcc aaccccttcc ggcgtaccgg tgaagatcag
     3241 atcgcccggt tgcagctcaa agaagcctga cagatagcag atggtttcat tcaccgacca
     3301 gatgagatgg cgaatatcgc tgcgctgatg atcttcgccg ttaacctgta accagattgg
     3361 cgcattatca ataccgccga tatcgcgggc tttgtgcagc ggcgcgatgg gcgctgagag
     3421 atcaaacgct ttaccgattt cccacgggcg gcccatctgg cgcatttcca tttgacggtc
     3481 acgccgagtc atatcaagac ccgtggcata accccagaca tattcatggg cgtgttccag
     3541 cgggatatcg ctgccttttt taccaatcgc caccaccagt tcaatttcgt aatggtagtt
     3601 atcggtctgt gccggatacg gcagcgccag cgtttcacca gcggcaaccg gaacaacggc
     3661 atccgccggt ttgcagaaga agaatggcgg ttcacgatcc ggatcaaagc ccatttcgcg
     3721 ggcgtgagcg gcatagttac gtccgacgca gtaaacgcgg cgcacgggga attgttcatc
     3781 actgccaaca accggtacgg taacgggcgc ttgcggttcg aatacgtatt gagtcatttt
     3841 tctctcctaa attaataacg cgcttcgcgg aacagcccca gggcttcttg taccggtctg
     3901 tccgaaaaac taaataaaac ggaatcttgc gtggtctgga acgacacgcc atgccaggtc
     3961 ggcaccacga agatatcttt tgcactaaag ctgaaggttt cattgccgat gatgacctgc
     4021 ccgctacctt ccaccacatg gtagatggtg ctgtcagtgg tacgcgcaac gcgtgaggca
     4081 aaccctttcg gcagcagttg caggaaagcg cccatcgacg gcatcggata gccgccggtg
     4141 accgggttga cgtagcgcat tttgtagcca tcccactcgt cggcatcgcc cagtcgggtt
     4201 aaatcgtgca gcacttcgcg gctgcggtca taacgatagt taaagatcgg cgaggagttc
     4261 cccgtctgat gccgcagcgg caacatattg gcagcgtaac gcggcagata atctccctct
     4321 ttacgcgtta ccggttgttg ctcttccgga taatcttcgg caaaaccgca gcccagaata
     4381 ttcactaatg gcagatccag cccatcgagc cagataaccg gttcgtcgcc agggttaccg
     4441 tgatcgtgcc agcgccactg cggggtcagg ataaaatcgc cttcattcat tggcgtgcgt
     4501 tcgccatcaa cggcggtaaa tgcccctttg ccttcaacaa taaaacgcag cgccgactga
     4561 ttatgacgat gactcggtgc cacttcgccc ggcatgatca gttgcaaacc ggcatataaa
     4621 gtcgcggtaa tggaagactg cccgcgcagc gccgggtttt ccagcaccag cacgcggcgc
     4681 acggcctctt tcgcaccaat caggccgccg ctctccagta gcagcgggcg gatctcctga
     4741 taattccagt acgctggcgc acagttagcg ttgggcgttt tcggcaccag atggtgcagt
     4801 gactcccaca gcggggtcag gttttgcccg gaaatatgct gataaaattg ctcgcggcta
     4861 ttttgattgt tatcagtcat tgagatgctc cttattcgtt cacaacgcct gcgcgaggca
     4921 gtgagtcagg gacagaggtc gactggcgaa ccacggtaat cagcagggtt aacatcaccg
     4981 cactgatcgc cgcaggcaca gcgataatga aaaacagggt gtcgaaagag aaattcatcg
     5041 ccatcatcac gccgccagac agcgaaccaa caatcgcgcc gcagcggccg atagcatttg
     5101 accagctcac gcccgtggct cgactttgtg ttggataaag tgttgcggtc agcgcattca
     5161 gccccacctg cgatccgcta atgccaatgc cagtgccgaa aatcgccagc gccatcaacc
     5221 ataatccatc ctggcttaag ccaatcatca cgatgcatat agcgcctatg gcatagctca
     5281 gcgtcagcac gcggaaagga ttgaacttat ccatcagcac acccagcgcc agcgcgccta
     5341 acgtaccacc aatctggaac gcggcggtga cccaggacgc gtgttgcagg tcgatgccgc
     5401 gatggttcag tagcgtcggc atccagcttg agagcaggta aataatcagc aggctcataa
     5461 agaacactac ccacagcatc aacgtgatgg gtaactgacg tcccataaac agttgacgaa
     5521 tgctgccttt tgtaacggag gctgattcaa tgagataaaa gtgggtatcg acataacgct
     5581 cgcgggtgat ggcgctgacg gttttcgcaa tcaccgcctg cggtagctgg cggcgcacct
     5641 gccagcgtgg cgattccggc agaactacca gcagtgcgac aaacagcatc agcggtaaca
     5701 cgccccccaa caccagaatg ccgtgccagc caatcaccgg caccagctgc gcactgacaa
     5761 tcccgccaaa cgccgaaccc agcgtaaagc cgcaaaacat cagcgtcacc agcgcgccgc
     5821 ggcggcgggc aggcagatac tccgaggtca tggtgatggt gttcggcatc gcgccgccca
     5881 gacccaggcc ggtgagaaag cgcaggaaga ccagcgtttg cagatccggt gagaaagccg
     5941 aggcgaggct taacgcgcca aacaaaaaga cgcacagctc aatcacccgc ttgcgaccaa
     6001 agcgatcgga caacgggcca caaagcagtg cgcctgcggt taatcctagc agacccgcgc
     6061 caaaaagcgg ggcgagatcg cctgcggtga gttgccagtg agtgcgaata tcaggggcga
     6121 taaatccgat agccgcggtg tcaaaaccat cgagcattac caccagaaaa cagcagatga
     6181 taacgcgcca ctgcatcttg ccgaccggag cagcatcgat aagtgcttgt aaatcacgtc
     6241 gttgagtcat agtaaatgcc tcagtgcagg taaggtagat gtttcttttg ttttgtatat
     6301 gttgcgatat tgtgattaaa gagatgagat gtacaatggc atttcgggca gagagataac
     6361 cttgaggtta tggcaaatgg cgaactgggc gcaaaaatta aaactacatc accttgaaat
     6421 gctggtggcg ctgggtgaac agggcaatct aacccatgtc gcacgaatga tgaatatcac
     6481 ccaacctgcg ctttcgaaat ggttgtcaca actcgaagat gagattggaa tcacactttt
     6541 tgagcggcac agcaaaggat tacgtccgac tgaaggcggc aaattgttat tgcaacatgc
     6601 ccaacggtta atcaatgaca tggagcgatc gcaacatgaa ctggcccgtt ttaaacaagg
     6661 tggactggtc ggcagtctga aaattggctg ttctcccgtc gccactgact gcgtatccca
     6721 ggcgattttg catttgctta cgaagatgcc aactctacac ctgaatatcg aagagaaggt
     6781 gatgacccca ttgctgcaag atctattggc cggttctgtc gatgtggtcg ttgggcgaat
     6841 tggcggtcgc gcattgcagc tgccactgaa ttatcaggtg ctgtacaccg agccagtatg
     6901 ctttgtcgcc cgccctgagc acccactggc gaaatacgcc accctcagct ggaatgatct
     6961 tgccaactgg cgctggatcg tctggccgac gggtaccccc attcgcatta gtattgataa
     7021 tgcgctggta gaccacggcg taatgctgcc agaaaacacg attgagtcgg cgtcgatgaa
     7081 cgtcagtacc aacctgttac aaagcagcga tatgatctct attttgtcat tacgcctggc
     7141 gcaacgttat gccagccagg gacaactggt gatactcaac ctgccaaaga tagaacagaa
     7201 aggtagcgtc ggtgtgttct ggcgcaataa cgaaacgcct tcttatgctt taagtcagtt
     7261 tctgcacttt ttacagcaga attagtgtgc tgaacggggc gctcgcccga ggttacctcc
     7321 ctaaaattca actatgctga gaattcttaa tgtgatcggt agcacgtttt acgaattaat
     7381 tgtatgatga atccatctca tctggggtgt tgattatgag taagacactg aacattatct
     7441 ggcaatattt acgcgctttc gtcctgattt atgcctgcct gtatgcaggc attttcattg
     7501 cttccctgct accggtaacc attccgggca gcatcatcgg gatgctgatc ctgtttgtcc
     7561 tgctggcctt gcaaattctt ccggcgaaat gggtcaatcc ggggtgctac gtactgattc
     7621 gctatatggc gctattgttt gtgccgattg gcgtaggcgt catgcaatat tttgatttgc
     7681 tccgcgcaca gtttggcccg gtagtggttt cctgtgcaat cagtacgctg gtggtttttc
     7741 tggtggtgag ctggagttcg caactggtac acggtgaacg taaagtcgta ggtcagaaag
     7801 gatcagaaga atgatggcga atatctggtg gtcattaccg ctgacgttga ttgtcttttt
     7861 tgccgcccgc aaactggcgg cacggtataa gtttcccctg cttaacccgt tgctggtagc
     7921 aatggtggtg atcattcctt ttttaatgct gaccggcatc tcttacgaca gttactttaa
     7981 aggtagcgaa gtgctaaacg atctgctgca accggcggtc gtcgcgctgg cctatccttt
     8041 atatgagcag ctacaccaga tccgcgcgcg ctggaaatcg atcatcacca tctgttttat
     8101 cggcagcgtg gttgcgatgg tgacggggac ttccgtggca ttgttgatgg gcgcttcacc
     8161 ggaaatcgcc gcgtcaatcc tgccgaaatc agtcaccacg cctattgcaa tggcggttgg
     8221 cggcagtatt ggcggtattc cggcaatcag cgcagtttgc gtgattttcg tcggcatcct
     8281 cggcgcggta tttggccata cattgcttaa tgcgatgcgt attcgtacca aagctgcgcg
     8341 cggtctggca atggggactg cctcgcacgc cctcggtacg gcgcgctgcg ccgagctgga
     8401 ttatcaggaa ggtgcattta gttcgctagc gctggtgtta tgcgggataa ttacttcgct
     8461 gatcgcaccg atccttttcc cgattattct ggcagtaatg ggctaaaatt tgcgatgcgt
     8521 cgcgcatttt tgatgtaagt ttcacgcgtt gcataattaa tgagattcag atcacatata
     8581 aagccataac gggttcgtaa actgttatcc cattacatga ttatgaggca acgccatgca
     8641 tccacgtttt caaaccgctt ttgcccaact tgcggataac ttgcaatctg cactggaacc
     8701 tattctggca gacaagtact tccccgcttt gttgaccggg gagcaagtct catcgctgaa
     8761 gagcgcaacg gggctggacg aagacgcgct ggcattcgca ctacttccgc tggcggcggc
     8821 ctgtgcgcgt acgccattgt cgaattttaa tgttggcgca attgcgcgcg gtgtgagcgg
     8881 aacctggtat tttggtgcca atatggaatt tattggtgcg acaatgcagc aaaccgttca
     8941 tgccgaacaa agcgcgatca gccacgcctg gttgagtggt gaaaaagcgc ttgcagccat
     9001 caccgttaac tacacgcctt gtggtcactg ccgtcagttt atgaatgaac tgaacagcgg
     9061 tctggatctg cgtattcatc tgccgggccg cgaggcacac gcgctgcgtg actatctggc
     9121 ggatgccttt gggccgaaag atctggagat taaaacgctg ctgatggacg agcaggatca
     9181 cggctatgcg ctgactggtg atgcgctttc tcaggcggcg attgcggcgg caaaccgttc
     9241 gcacatgcct tacagtaagt cgccaagcgg tgtcgcgctg gaatgtaaag acggtcgtat
     9301 tttcagcggc agctacgctg aaaacgccgc attcaacccg actctgccac cgttgcaggg
     9361 agcgttaatt ctattgaatc tcaagggtta tgattacccg gatatccagc gcgcagttct
     9421 ggcagagaaa gccgatgcgc cgttgattca gtgggatgcc acctccgcaa cactgaaagc
     9481 tctcggctgt cacaatatcg accgcgtgct tctcgcttaa gcctggtgcc ggatgcggcg
     9541 tgaacgcctt gtccggcttg ccagccctct cctggtgtcg aaaatcccgg caaacagttt
     9601 gtcgtttctt gcgcaaaacc agcgggtaaa gtagcctgat ggaaattttc cttagatcga
     9661 gtctcctgca tgttaaagcg cgtgttcctc agcctgttag tcctgatcgg cttgctgctg
     9721 ttgactgtgc tcggcctcga tcgctggatg agctggaaaa ccgcgcctta tatctacgac
     9781 gaattgcagg atctccccta ccgccaggtc ggtgtggtgc tcggaacagc aaaatattat
     9841 cgtactggcg taattaatca gtattatcgc taccgcattc aaggagcgat taatgcctat
     9901 aacagcggta aggtaaatta tctattactg agcggcgata acgcattgca aagttataat
     9961 gagccgatga ccatgcgcaa agatttaatc gctgctggtg tcgacccatc agatattgtt
    10021 ctcgattacg caggctttcg tacgctggat tccatcgtgc gtacacgcaa agttttcgat
    10081 actaatgatt tcattattat cacccaacgt ttccactgtg agcgggcatt atttattgcg
    10141 ctgcatatgg ggattcaggc tcagtgttat gccgtaccgt caccgaaaga tatgctgtca
    10201 gtacgtattc gtgaatttgc cgcccgtttc ggtgcgctgg ctgaccttta tatttttaaa
    10261 cgtgaaccgc gttttttagg gccgctggtc cctattccgg ctatgcacca ggtgccggaa
    10321 gatgcgcagg ggtatcccgc cgtcacaccc gaacagttac ttgaattaca aaagaaacaa
    10381 ggaaagtagt tatggatgta cagcagtttt ttgtcgttgc cgtttttttc cttatcccga
    10441 tattttgttt ccgcgaagca tggaaaggct ggcgcgcagg cgcgattgat aaacgggtta
    10501 aaaatgcacc ggaaccggtg tatgtctggc gagcaaaaaa tcccggactc tttttcgctt
    10561 atatggtggc atatatcggc ttcggaattt tatctatcgg catgattgtt tatcttattt
    10621 tctatcgtta atattttctc ttttctgtta attattgaga ataattatta cttcactcaa
    10681 taagctgcgg atataacatt ctgaaccgca gctatttgtg aatcctttca cagtttaaat
    10741 ttccccacgc gcatagcctt aatatcagca cgcaatattt atttagcgtt cgccttaatt
    10801 actcatagca ttaaggaaga tcacatgccg caacaaaatt atctggatga actcactccg
    10861 gcttttacgc cgttactggc gattaaagaa gcctctcgct gtttattatg tcacgacgct
    10921 ccctgtagtc aggcttgccc ggcacagacc gatccgggga aatttattcg ctcaatctac
    10981 tttcgtaatt ttaaaggcgc tgccgagaca attcgcgaaa ataatgccct cggtgccgtt
    11041 tgtgccagag tgtgcccgac ggagaaatta tgccaaagcg gttgtacccg tgccggtgtt
    11101 gatgctccca ttgatatcgg ccgcttacag cgttttgtta ctgattttga acaacaaacc
    11161 gggatggaaa tttatcagcc cggtactaaa acgctcggca aagtcgcgat tattggcgca
    11221 ggtcctgccg gattgcaggc cagtgtgacg ctaacaaacc agggttatga cgtcacgatt
    11281 tatgagaaag aagcgcaccc cggtggctgg ttgcgtaacg gtattccgca atttcggtta
    11341 ccgcaatcag tgctggatgc agaaatcgcc cgtattgaaa aaatgggcgt gaccattaag
    11401 tgcaataacg aaatcggtaa aacactcacc ctggagcaac tgaaagcgga aaaccgcgcg
    11461 gtactggtca ccgtggggtt atcaagcggt tccgggctac cgctgtttga gcatagcgac
    11521 gttgagattg ccgtcgactt cctgctacgt gcacgacagg cgcaaggcga tatcagcatt
    11581 ccacaaagcg cattaattat cggcggcggt gatgtcgcga tggacgtagc cagcacgctg
    11641 aaagttctcg gctgtcaggc ggtaacttgc gtagcgcgtg aagagttaga tgagtttccg
    11701 gcaagcgaaa aagagtttgc cagcgcccgg gagctgggcg tttcgatcat cgatggattc
    11761 acgccagtag ccgtggaagg caataaagtc acgtttaagc atgtacggtt atcgggcgaa
    11821 ctgacgatgg cggcagataa aattattctc gccgtcggtc agcatgccag actggatgac
    11881 tttgcgaagt tagagccgca gcgtaacacc atcaaaacac aaaattatca gacccgcgat
    11941 ccgcaagtct ttgctgctgg cgatattgtt gagggtgaca aaaccgtggt ctatgccgtg
    12001 aaaaccggga aagaagccgc cgaggcgatt catcactatt tagagggagc ttgctcatgt
    12061 taacgaaaga tctttcgatt actttttgcg gcgtgaagtt tcccaacccg ttctgcctct
    12121 cttcttcgcc ggtaggcaac tgctatgaga tgtgtgccaa agcctacgac acaggttggg
    12181 gcggcgtggt gtttaaaacg atcggctttt ttatcgccaa cgaagtctcg ccgcgttttg
    12241 atcatctggt gaaagaagat accggtttta tcggcttcaa aaatatggaa cagattgctg
    12301 aacatccgtt ggaagagaat ctggccgccc tgcgtcggct gaaggaagat tacccggaca
    12361 aagtattgat cgcttcgatc atgggggaaa atgagcagca atgggaggag ctggcgcgcc
    12421 tggtgcaaga agctggcgcg gatatgatcg agtgtaactt ctcctgtccg caaatgactt
    12481 ctcatgcgat gggtagcgat gtcgggcaaa gcccggagct ggtagaaaaa tattgtcggg
    12541 cagtgaaacg gggttccacg ctgccaatgc tggcgaagat gacgccgaat atcggtgata
    12601 tgtgcgaagt ggcgctggcg gcgaagcgcg gcggcgcaga tggcattgcg gcgattaaca
    12661 ccgttaaatc catcaccaat atcgatctta atcagaaaat cggtatgccg atcgttaacg
    12721 gaaaatcgag tatttccgga tattccggta aagcggtaaa accgatcgcc ctgcgcttca
    12781 ttcagcaaat gcgcacccat ccagaactgc gcgatttccc aatcagcggt atcggcggca
    12841 ttgaaacctg ggaggatgcg gcggagtttt tattgctcgg cgcagcaacg ttacaggtca
    12901 ccaccggcat tatgcagtac ggctatcgca tcgtagaaga tatggcgagc gggttatcgc
    12961 attatctcgc cgatcaggga tttgattcgt tgcaggagat ggtaggtctg gcgaataaca
    13021 atattgtccc ggcggaagat ttagaccgca gttatattgt ctatccccgt atcaatcttg
    13081 ataaatgtgt tggctgtgga cgctgttata tttcctgtta cgacggcggt caccaggcga
    13141 tggaatggag cgagaaaacc cgcacaccgc attgtaatac cgagaaatgt gtgggttgtc
    13201 tgctttgtgg tcacgtctgc ccggtgggtt gtattgagct cggggaagtg aagtttaaga
    13261 aaggcgagaa agaacacccg gtaacgttgt aaaaccagag acgcatccgg catttggttc
    13321 ctgtcgccgg atgcggcttg aacgccttat ccggcctaca aaaccataca aatcagtatg
    13381 ttgagctcat tgcaggccgg ataaaacgcg ccagcgttgc atccggcatt tggctcctgt
    13441 cgccggatgc ggcgtgaacg ccttatccgg cctacaaaac cacacagatc atgatgttat
    13501 gtgcgtttat gaccgaatgc ggaccacaat catttcactt cttacgcgca tacttcagtg
    13561 aatccagcgc tacggcgaag ataataatcg cccctttgat gatgtactgc cagtatgggt
    13621 ttacgccgat gtacgtcagg ccatagttga tgacggtaaa aataattacc ccggtcacca
    13681 cgccaatcac cgtccccaca ccgccgctga acgatacacc gcctaccacg cacgccgcga
    13741 tagcatccag ttcatacata aagccgaggt tgttggtggc agagccgata cgtccggctt
    13801 ctaacatccc gccaaaggca tagaacacgc cagacaacgc gtagatcatc agcaggttca
    13861 ggccgacgtt gacaccagat acttttgccg cttccgggtt accgccaatg gcaaaaatgt
    13921 tcttaccgaa gcgggttttg ttccacaaca cccagacgaa cgccaccgca atcaacgcgt
    13981 agaaggtgat gtaagagaga cggaaactcc ccagcgcgac aaagccctga gcaaaggtag
    14041 agaagccact gtcaaaacca gaaattggcg acgccccgac aaagtcgtaa taaagcgagt
    14101 tgatgccata gacgatgatc atcgtgccga gcgtggtaat gaacggcgtc acgttgagat
    14161 aagcgataat cagaccgttg atcaaaccga tcaccgcacc aatggcacag acaatcagaa
    14221 taaccagcgc aatcggcatc gtcgccattt ccgggaacac tttgttggcg ttatccatgg
    14281 actgcaataa tgtcgctgcc accactgccg ccagccctac ttgacgcccc gcagaaaggt
    14341 cggtcccctg ggtgacaatt aaccctgcca caccgagcgc gataataata cgcaccgatg
    14401 actgggtgag aatattactt aagttcaaca gacttaaaaa tgttggggcc tggaaaataa
    14461 taatcgcaag caaaactaaa agaacgacgt aaataccgcc ctctttcagg taagtaagaa
    14521 aacttttctt atttaacgca ctcatgggaa gcccctaatc ttaaaggtgc aaagacgcaa
    14581 gacgtagaat ttcgttttgc gttgttgttt ttgtatcgac aattccggaa acgaggccat
    14641 tgctcataac cagaatacgg tctgttatcc ctaacaactc tggcatttca gaggagataa
    14701 taataatccc cttgcctttc ttcgccagtt cggcaattaa ctgataaatc tcaaacttcg
    14761 ccccaacatc aataccgcgc gtcggttcat cgagcattaa tatttctggt tgcgttaaca
    14821 gccagcgccc aataatcacc ttttgctgat taccacctga gagcgaacca atttgcgtcc
    14881 gatgccccgg tgtttttacc cgcatcgaat caatcaccca ctgggtatcg cttttcatcc
    14941 gcgagttatc cagtaaacca actttatttt tgtagttgcg aatattggaa attaacgagt
    15001 taaaaccaat atccagatag gcataaattc ccgttgagcg gcgctcctca gttaccagtg
    15061 caaatccgtg gtttatggct tcgttggcat tatggttatt gatctttttg ccgtgcaacg
    15121 taatggtgcc agccgatttc tcgcgaatac caaataacgt ctcaacaata tcggtacgtt
    15181 tcgcccccac cagaccggca ataccgagga tctccccttt atgcagatca aacgagacat
    15241 cgcgaatcga cggctggcgc agtgacgtca ggttacgtac ctcgaggatg acttcgcccg
    15301 gcttgttttc tttgtcagga aaacgctggt taagagaacg cccaaccatc atggcgataa
    15361 tcttgtccat cgtcagccct gccagcggtt cggtggcgat ccactgaccg tcgcgcaata
    15421 cggtaacttc atcacataac tggaagattt cctccatttt atgcgagata taaacaatac
    15481 cgcagccgcg ctcttttaat ttacgaataa tagtgaacag atgattgacc tctttttcgg
    15541 ttaacgaaga agtcggttca tccataatca caattttcgc gttataggaa aacgctttgg
    15601 cgatttcgat catctgcatt tgcgaaacag ataatgtgcc gacgcgcgca cgcggatcga
    15661 tatcaatatc cagttcatca aaaatcgctt tggtttcgcg gtacattttg tcctgatcga
    15721 caaacatgcc tttggtgggg tatcgcccaa gccacatgtt gtccatcacc gaacgttgta
    15781 ataccaggtt taactcctgg tgtaccatcg aaataccatt ttccagtgct tctttggccg
    15841 aatggaaatc gatctcttta ccctggaata aaatggtgcc ggagtctttt ttataaatac
    15901 caaacaggca ttttaataat gttgattttc ctgcgccgtt ttcccccatt aatgcatgga
    15961 tagaatgtgg ccggactttt aaattaacgt tatcaagtgc cttaacacca ggaaaagact
    16021 tgttgatacc gctcatttcc aacaagtatt ccccggaaga cggagtcgtt gagctgacca
    16081 tataatttta ccttgttggc catacaataa gggcgcagta atagactgcg cccaatcagt
    16141 cttatttctt gctgaattca gccaggttgt ctttatctac gccaacataa ggtacgcgga
    16201 ccactttgtt gtcgattttc cagttggtgc catcagccgc acctttacca tcggccaggt
    16261 ttttcgccag atcaaaggtc gctttcgcct ggttgttagc atcgttcagt acggtgcccg
    16321 ccagtgcacc ggatttcacc agcgccagcg cttctggcag agcatcgacg ccaaacaccg
    16381 gaatgctgga cttgttgtgt gctttcagtg cttctaccgc gcccattgcc atcgcatcgt
    16441 tgttggcgat aaccacttcg attttgttgg cgttcgggcc agacagccag gcgtccatct
    16501 tatctttcgc ctgagcggta tcccacatag cggtatctaa ctgtaactgt tcagttttaa
    16561 tgcctttgtc gttcagctct ttaatcacgt aagtggtacg tgcttctgca tccggatggc
    16621 ccggttcacc tttcagcagt acgaattgaa tctgaccgtc tttattcaga tcccaaccct
    16681 gattcgccgc ccagtgttta gcaatcaaat cgccttgaat aatgccggac tctttggagt
    16741 cagtgccaac gtagtaggct ttgtcgtagc tatccagcgc cttacgagac ggttctttgt
    16801 taaagaatac caccggcaca ttttgcccac gcgctttctc aatcaccgta cccgcagctg
    16861 ccgggtcaac caggttgatt gccagtgcct tcaccccttt cgccagcaat acgtcgatct
    16921 gatcgttctg cttggactgg tcattctgtg aatcattcat cagcagctga acatctggcg
    16981 cggctttcgc atcttgctca atagccttgc gcactacaga cataaagtta tcgtcgtact
    17041 tatagattgt tacaccaatg cgagtatcag cagcgtgtgc agtggcaccg aataacatgc
    17101 tggccatcac agcagacagg gttaacacct tcttattcat ggtatctccg gtttttctta
    17161 tgcagggtag tgcttgagat aaatgctcgg cggggcagta gagttaatga agtgttactg
    17221 aacgccgaag ctcacttttt aaaattcgtt cttccatgcc cggtaacgct ccagaaaacg
    17281 gctttaattg ttgttatgac gcattacctc ggtgaaagtg attattcact gttacatacg
    17341 ggttacaacg ttaaaacggt gcaatcatag ctatcacatt gttaagatac tgtgaaatca
    17401 ctcacagatt gaaagcggtt acatcgcctg atttgttgag ttagtgatcg ccgccgcatt
    17461 ctggcgtgtt gcgacagaat ggcgacgcac taacgtcggc ataaaacagt ggctggcacg
    17521 aggatcaata ttgcctgctg ccccctgcaa ggccagttcg gtggctaatt tcgccattga
    17581 agcaatggga taacgcacgg tcgttaattg cgggtcggtg taacgggcga tgggaatatc
    17641 atcgaaaccg atgattgaga gatgtaacgg aatcgcaatg ccattatctt ttaatgctgt
    17701 cagtgcgcca gcagccatat tgtcgttata agcaaataca gcggtaagtt gtagattgcg
    17761 ccccagcagt tcaaccatcg ccgcctcacc gcccggcatg tccggcgtac cagtgccaat
    17821 ccagctttcc ggcggaataa tatcctgctc tttcaacgca ctcatccagc ctgctttacg
    17881 catggcgtca tcttcaatgc cgtggctgga agaaagataa ccaatacgtt gatgaccgtt
    17941 attcagcaac atgcgcgtcg ccattcgggc accgctgaga ttatccaggc aaacgcaacg
    18001 atgggtgtac cccggcacaa cgcggttgat taacaccata ccgggaatgt tatccataaa
    18061 ttgcgccagt tcatcgtcac tcaatgcttt tgagtgaaca atcaacgcat tacaacgctg
    18121 gcgaattaac acctcaatgg cgtgacgctc tttttccgct tcatgatagc tattgccgat
    18181 tagcacgtat ttctgatgct gctgagcgac cagatccacc gcttttacca gcgcgccgaa
    18241 aaacgcatca gaaacgtcca tcaccaccac gccaatggtg tcgctaacct gagttgccag
    18301 cgcctgggca ttggcgtttg gccgataatc cagctcactc acggctttca ttactgcttc
    18361 acgcgtgtcg gcactgacca gcgtgctgtt attgagcacc cgggaaaccg ttgccacaga
    18421 gacgccagcc tgacgcgcta catcacgaat ggtgatcata ttcactacct gttatgagat
    18481 tgcagtaaat gactgcttgc tgccggctat tttgtcagca ctgaatacag gacttcgtga
    18541 atcgagtcac agcaatggaa acggttacag ccgttttgtt aacgaatgtg atccagatcg
    18601 ttatcttgat gttttagata ttgccggtcc ggcagcacgc aaagttaact gacgccagag
    18661 ccattccacc ggcccctggc ggaagtaacg cagccagata acagagaaga gaatattcgc
    18721 cagccatacc ggaataacaa acgccagcaa ttccagacgg tcaaactgca taaacagacc
    18781 gaggtggtaa aaaagcgtgg tacaaatcag cgtttgcaat agatagttgg tcagcgccat
    18841 ccgtccgacg caggcgatcg ccagcaccag cttaaagcgg ctcaattgcg gccagaagcc
    18901 ataaaacagc gacgcatagc caatcgcctg aaacggcgca ctcagttccc gcggcatttg
    18961 aagtaagaag gcacaccagc gatacgccca gtccagctgc cattgcaggg cgatggcagg
    19021 aaggttaatg atcaccccaa tcgccactag cacaaaacca gtacgacgat agtgacgtaa
    19081 gctgaactgc cctttcagcc agccactgcg catcaatgcg gcaccaatga gcatcatccc
    19141 agccagttgc cagccatact gtgcgcccag tgccaataaa ctgttcccaa caccatcggc
    19201 acgattactg atcgcttcaa cgccgccgtg aagcttccag tatttttcat ataaaatagc
    19261 cgatgcatcc ggcgtccagg cgcggctagt ctggctatcg gaaatcaacc ccaataacag
    19321 caaaacgcca agccccacca gataaagcat gacgccggta ttaaaaaggc tttttaccga
    19381 tggcgcatcg cgcaccagcc gccagcagat taagcccacc agcccgtaag ccagcagaat
    19441 atcgccgtcc cagaacaata aaccgtgaat aaagcccagc aagactaaca gcgttaaccg
    19501 cgactggatc cagcgtctgc cacggggcag caacatttgc aggccagcac caaacagcag
    19561 cgcaaaaagc gtgaggaatt tcacctggcc gatgagatcg agaaatgccc aggtccatgc
    19621 atcctgcggc gtaatagcgc cgtaccaggc gggattaaga taagccgcct ttggtagccc
    19681 aaaggcgctg atgtttaata gcaggatccc cagaatggcg acgccgcgaa caaagtcgag
    19741 cgtgacgttg cgctccatgg ttcctgcctt taaatcagtt gtgatgacgc acagcgcgca
    19801 gaaactcgtg gcgcgtattc tgactggatt tgaacaatcc accaagagag gtcgttgtcg
    19861 tggcactggt tgcatcgcgg atgccacgcg ccttcacgca ataatgcacc gcgtcgatag
    19921 agacagccac gttattggtg cccagcagcg tttgtagcgc aatgagaatt tgctgcgtca
    19981 gacgttcctg cacctgcgga cgctgggcaa agaactgcac aatacggtta atttttgaca
    20041 gaccgatcac cgaatctttc gggatataag ccaccgtcgc tttgccatcg atggtaacaa
    20101 aatggtgttc acaggtactg gtcagagtga tatcgcgcac ggtgaccatt tcatcgacct
    20161 tcattttgtt ttcaatgagg gtgattttcg ggaagttggc gtaatccaga ccggagaaaa
    20221 tttcatcgac atacatttta gcgatgcgat gcggcgtttc catcaaactg tcatcagcca
    20281 ggtcgagatt cagcagctgc atgatttcgg tcatatgacc agcaataagg cttttgcgcg
    20341 tttcgttatc catttcatgc acgggcgggc gcagcggtgt ttccagtcct cgcgcaacta
    20401 acgcttcatg aaccagggcc gcttctttac tgagtgatgg catttatgat ttctcctgca
    20461 ggtgtgacgc ctccgccctg cgtgggggca aagttattaa gctgatttac agcctgatta
    20521 ttgtgcgtga ggcggcgcac ataatccagt attcacagcg ataattattg taattgccgc
    20581 tgcctttcat cagcagatgt taaaacatcg ttatgcaaat acggaagtga aagttactca
    20641 cagcacattg aataaacggt atgatgaaga aattgcaaac aacacaacaa ggagccacgc
    20701 atggaaatgc tcgaagagca ccgctgtttt gaaggctggc agcaacgctg gcgacacgac
    20761 tccagtacct taaactgccc gatgacgttc agtatctttc tcccaccccc tcgtgatcac
    20821 accccgccac cagtgctgta ctggctttcc ggattaacct gcaatgacga gaacttcacc
    20881 accaaggcgg gtgcccagcg cgtggcggcg gaactgggga ttgtactggt tatgccagac
    20941 accagcccgc gcggcgaaaa ggttgccaac gacgatggct acgatttagg ccagggcgca
    21001 ggcttttatc ttaatgccac gcaaccgccg tgggcgacgc attaccggat gtatgattat
    21061 ctgcgtgatg aattaccggc gctggttcag tcgcaattta atgtcagcga ccgctgcgct
    21121 attagcggtc attcaatggg tggtcacggt gcgctgatta tggcgctgaa aaacccgggc
    21181 aaatacacca gcgtttcggc ctttgcgcca attgtgaatc cgtgcagcgt cccgtgggga
    21241 atcaaagcgt ttagccgcta tttaggtgag gacaaaaatg catggctgga atgggacagt
    21301 tgcgcgctga tgtatgccag taacgcgcag gatgcgatcc cgacgcttat cgatcagggt
    21361 gataacgatc agtttcttgc tgaccaactg caacctgcag tacttgcaga agccgcgcgc
    21421 cagaaagcat ggccgatgac gctgcgtatt cagccgggat atgatcacag ttactacttc
    21481 atcgcctctt ttatagagga tcacctgcgc ttccatgcgc agtatttact gaagtgaaag
    21541 tccgcccggt tcgccgggca tcttctcatc agaagcgata atccactgcc ataaagtaac
    21601 gacgtccgtc ttcgttatag ctgtagtcgt cacgactgag atccttgtcg ccaaggttca
    21661 gcacgcctgc gcgcagtttg acgtctttag tcacctgcca ggccgcgccg gtattccaga
    21721 tggtataacc gcccggtgtt ttagccgtcg cgctgtcggc tcgtttctgc ccggtatagt
    21781 gaccagaaac atagaatgac cagtcttcca gcgccagcgg tttccagtcc agcgtaccgt
    21841 tagcggtatg gaacggcaga tcggatagcg gtttgttttc gccgttgctg acatcacgac
    21901 catcgttgta ggtgtagttg agtgacagtt tccattcatc gttgaacgga attttcagct
    21961 cggtttccac gccctgaata cgtgctttgt taacgttgta gtagctaaat accggcacac
    22021 cgttcgctgt aaaaccaaca aagttttggt agcccggtgc agcattgacg tcagacgtac
    22081 ggctgatgct gatacgatct ttcacatcgt tacggaaaac ggtaacgctg gattcaaccc
    22141 cttccagcca gccttcttca cccatgtagt aaagccccag ctcccaactt tcgctggttt
    22201 ctggtttcag atccgggcta cccacaatct tacatgcgcc acggcaggaa ttgctcgtcc
    22261 agtcagggct aagttgcaac agagaaggtg ctttaaatgc cgtcgcccag ccccctttca
    22321 ccgttacggt gtcggtggcg ttataaacca ggtaggcacg cggactccag tgttcaccgt
    22381 aggtttcgtg atcgtccata cgcacgccgg tcgtcagcgc cagcggctcg aagatccgcc
    22441 attcatcttc cacaaacagc gcgtactggc tggcagacgt tttggagctg gttcccccgg
    22501 tcaggttcac cgcatcgcta agtttgtcgt gacgccattc accgccaacc gtgagaaact
    22561 gattaatcgc cgtcagcggc aacgtgtatt tgccgtcgac cgtattgctt tcggaagtta
    22621 tcgggctgct gttgccaggg tttttgttct cgactttctc accgtagtat ttcagttcgc
    22681 tggtgccgta atcccaacgc ccattatggc tgacggagta gttctggcgt tccaggcggt
    22741 ttttgtccag cgagtcggaa tcacgatcct gacggtcgaa accgtatccg gcagtaaaat
    22801 cgtgattttg attcggtgtc caggcaaatt cgacattgcc gtcgcggctg gagaatcctt
    22861 caatacgcgg cgtttctccg gtatcggtgg tcgttgagtt ttgcgggtca tccttttcac
    22921 gttttgccag gctgccgtaa gctttcattc ccagcacacc atcaattaat ggtccactgg
    22981 taaagaactg accgttatag gtatcaccgc gatcgcgatg ttcctgaatg gtggtatcga
    23041 cggtaacggt acccgaccat ttctgaccga tttttttggt gatgatattc actacaccgc
    23101 cgagcgcatc ggaaccgtac agcgacgaca tcgggccacg gaccacttca atacgttcga
    23161 tggaatcgac cgggatccag ttcagatcga aatcattgtg gcggaagacg gcattgcggg
    23221 agttaacgcg tttaccgtcg acaagaatca gggtgtagct gctgtccaga ccacgaatac
    23281 taacgccctt acggttatcc ccttcgttcg tcagttgtac gccaggcact tctttgagga
    23341 catccttcag attctgtacc ggttttcgct gcaggtcttc ctgggtaatg acgctgatac
    23401 tggcgggtgc atctttaagg ttttgttcaa cggaagatgc agtgacaacc atcgtttcgc
    23461 catcgtcatc gaccgctaac acaggccatg cacaagaaat agcggacaaa cacagcccga
    23521 cccgtacgaa agggttcaac ctaaacattc catatctcca tgaggtaact acgaaaataa
    23581 aatgggttat cgctcacatc ttcttcacgt ccccttgcgt acggcagcat cgcggtggac
    23641 ttatttgtat gcaggtgatc atcccagaaa gccggaccag cctctgattt gtcagccttt
    23701 tttgattgcg gcgtaacgat aatgcaaacg ataacaatta tcaatccaaa tgttaaattt
    23761 tatatccgca tgatttgtca gggaaatgga attgaaaaag ccctctcggt tgagagggct
    23821 tagcaaggaa gggagaaact tatttcttat cgttctgcgg gaacttcatt tcgctgtagc
    23881 gtacgaagtg ggttccttta atcagcttgt agccgaacca aataatcagg aacagcggga
    23941 taccaatata cgttgccgct acgccgcccc agtcaatggt atctttcagg aacgcttcgt
    24001 agttctggcc caaagtgata atcagacaca gaatgaatgc gaagatcggc cccaatggga
    24061 agaaacctga acggtacggc agatcgttaa tgtcgtgtcc ctgcaatacg taaccgcgac
    24121 ggaagcgata gtggctaatg gcaatcccca gccaggcgat aaaaccggtc atcccggagg
    24181 tattcagcag ccacaggtat accgtctggt tgccaaacat ggaggtcagg aagcacagac
    24241 cggcaatcac cgtcgtcgca tacagcgcat tacgcggcac gccaccacgc gacagtttag
    24301 cgaaaatgcg cggcgcttta ccgtcacacg ccagggtgta cagcatacga gtagacgcat
    24361 acataccgga gttacccgct gacagcaccg ccgtcagaat aactgcgttc atcaccgccg
    24421 cagcagagag cagacccgcg tgctggaaca ccagggtgaa cggactaacg ctgatgtctt
    24481 taacatcgtt acgcagcagg ctcggatcgg tgtacggaat aatcaggctg ataatcagga
    24541 tcgcgaacac atagaacaac aggattcgcc agaacacctg acgtaccgcg cgtggaatgt
    24601 ttttccccgg atcttcggac tcgcctgcag caataccgat cagctcggtt ccctggaaag
    24661 agaagccgac aatcatagct acgccgatca tcgccgcaaa accaccagca aacggcgctt
    24721 cgccgattgt ccagttgctc cagcccgcag gctgcgcgcc tttgaagata ccgataatca
    24781 tcagcacgcc aacgatgata aagacaataa ctgtcgtgac tttgatcagt gagaaccagt
    24841 attccgcttc accaaagccg cgaacagaga tgtagttcag caggaagata acgccgagga
    24901 acaacgcact ccagatccag cccggtgtat ccgggaacca ccagctcatg accagctgag
    24961 ctgcaaccag gtcaacggca atagtcaccg cccagttgta ccagtagttc cagcccagcg
    25021 cgaagccaaa gccctcttca acatagttct gaccgtaagt ggcaaacgaa ccggaaaccg
    25081 gcatatatgc agccagttca ccgagactgg tcatcaggaa gtaaaccatc aggccaatca
    25141 acatatacga gagcaatgcc ccgcccggac ctgcctgaga aatcgttgcg ccagaggcaa
    25201 caaaaagacc tgtaccgatg gaaccgccaa tggcaatcat cgtcaggtga cgcgccttta
    25261 attcacggcg taagcccggc gcttctgtgg ttttagtttc ggaaaccata caaaaatgct
    25321 atccatctaa aaaatgaggc gcgattgtag cagacgattg gcaatccttc cggcagaaat
    25381 acacggttat aagacacctt catgatcgcc cagggattat aagtaaagca gccaatctct
    25441 tttctggcga atgcatgctg aaggcgctgc gtcacgaaat cgacacagca ccagcatgtt
    25501 cttgtacagc aacttaacgc ggcacatttg cgggatcgca atagtccaga aagcgccgta
    25561 gcgcgttgga aaggtgtttt tgccgatgat gtatacgcca caacgtacgc atcaggcgcg
    25621 gcagagggac cgcaacttca cttaatgtgc ctgcctgcaa ttgatcttca atcacacgtc
    25681 gcgacaggca actaattccc aacccatgac gcaccgcatg tttgattgcc tcggagttac
    25741 ctaattccat cgccatctca aacttcggta aatgtgacag caacagataa tcgacaatct
    25801 cccgcgtgcc ggaaccgcgt tcacgcagga tccacggcgc agcggccagc tgttctaagg
    25861 tgaccggacc acgggccaac ggcgaagtcg gcgcggcgaa aaccaccagc tcgtcttcca
    25921 gccacggttc agaaatgatt tcagtgctgt ggcacggtcc ttcaataaag ccaatatcaa
    25981 cgcggaaatc cagcaccgct tgcatcacgt cctggctatt cccaacgcta agttcaatcg
    26041 gcaactgcgg ataatcatgg cgataacggg cgataactgc aggcagaatg tagttaccga
    26101 tggtactact ggcatagata cgaatcgcgc cgttgtcttc gcgaaacagt tgttcgattt
    26161 caaccgcctg ttcaagcaat gccaacgcac gcggatagag cagccgcccg tgttcattaa
    26221 caaccagtct tttccccacg cgatcaaaca gttgcacgcc aagctgccct tccaggtcgg
    26281 tcaaggctgc gctcactgct gattgcgaca acgccagcat caccgacgct tgggtggttg
    26341 atccactttt caatacttct gcaaaaactt ccaactgccg gagggtgatg tgcatagtcg
    26401 cttaccactt ataaagatta attataaata tataatcaat tttattttta aaccagttag
    26461 tcgtaacctt atgcccggtt aaaggagagg gttatgacga atatcacctt acagaaacaa
    26521 catcgtacac tgtggcattt tattccgggg ttagccctga gtgcagttat caccggggtc
    26581 gccctgtggg gtggttccat tcccgcggtt gcgggtgccg ggtttagtgc cctcaccctc
    26641 gcaatcttgt tggggatggt tttaggcaac accatctatc cgcacatctg gaaaagctgt
    26701 gacggtggtg tgctgtttgc caagcaatat ttattacgtc tgggtatcat tctttatggc
    26761 ttccgtctga cgttctcgca aattgccgat gtcggtatca gtgggatcat cattgacgtg
    26821 ctgacgctgt ccagtacctt cctgctggct tgcttcctgg ggcagaaagt gtttggtctg
    26881 gataagcaca ccagctggtt gatcggtgcc ggtagcagta tctgtggtgc tgccgcggta
    26941 ctggcgactg agccggtagt gaaagcggaa gccagtaaag taaccgtggc tgttgcaacc
    27001 gttgttatct tcgggaccgt cgcgattttc ctctacccgg cgatatatcc gctgatgtct
    27061 caatggttta gtccggaaac tttcggtatc tacattggtt ctactgtgca cgaagtggcg
    27121 caggtggtgg cggcaggtca tgccatcagc ccggatgcgg aaaacgcagc agttatttcc
    27181 aaaatgctgc gcgtgatgat gctggctccg ttcctcatcc tgctggcggc gcgtgttaaa
    27241 cagctgtctg gggcgaacag cggcgagaaa agcaaaatca ctattccgtg gtttgccatc
    27301 ttgttcatcg tagttgccat ctttaactcg ttccacctgt taccgcagag cgtggtgaac
    27361 atgctggtaa cgctggatac cttcctgctg gcaatggcga tggcggcgct gggtctgacc
    27421 actcacgtca gcgcgctgaa aaaagctggg gcgaaacctc tgctgatggc actggtactg
    27481 tttgcctggc tgattgttgg tggtggtgcg attaactatg tgattcaaag cgtcatcgca
    27541 taaaccacta catcttgctc ctgttaaccc gctatcatta ccgttttcct ccagcgggtt
    27601 taacaggagt cctcgcatga aatacattgg agcgcacgtt agtgctgctg gcggtctggc
    27661 aaatgccgca attcgcgccg ccgaaatcga cgcaaccgcg tttgccttgt tcaccaaaaa
    27721 ccaacgtcag tggcgtgccg caccgctcac gacgcaaacc atcgatgaat tcaaagccgc
    27781 ctgtgaaaaa tatcactaca catcggcgca aattcttccc cacgacagtt atctgattaa
    27841 cctcggacat ccggtcactg aagctctgga aaaatcgcgc gatgccttta tagatgaaat
    27901 gcagcgttgc gaacagctgg ggctttcttt gctcaacttc caccctggca gccatctgat
    27961 gcagatttca gaagaggatt gccttgcgcg tattgccgaa tccatcaaca ttgcgctgga
    28021 taaaactcaa ggtgtgacag cggtgataga aaacaccgcc ggtcagggca gtaacttagg
    28081 gtttaaattc gaacatctcg cggcgattat cgacggcgtg gaagataaat cccgcgtcgg
    28141 cgtctgcatt gatacctgcc atgctttcgc tgccgggtat gatttgcgta ctccagccga
    28201 atgcgagaaa acattcgcgg attttgcccg tactgtcggc tttaagtatc tgcgcgggat
    28261 gcaccttaac gatgcgaaaa gcacctttgg cagccgcgtt gaccgccatc atagcctcgg
    28321 tgaaggcaat atcggtcatg atgcgttccg ctggatcatg caggacgacc gtttcgacgg
    28381 cattccgctg atcctcgaaa ccatcaaccc ggatatctgg gcagaagaga tcgcctggct
    28441 gaaagcgcaa caaactgaaa aagcggtagc ctgaagatga ataaccggga aaaggagatc
    28501 cttgcaattt tacggcgtaa cccgctgatt cagcagaacg aaattgcgga catgctgcaa
    28561 atcagccgtt cgcgcgttgc ggcgcatatt atggatttaa tgcgcaaagg ccggattaaa
    28621 ggcaaaggtt acattctcac cgagcaggaa tactgcgtag tggtggggac aatcaatatg
    28681 gatattcgcg ggatggcgga tatccgttac ccgcaatcgg cttctcatcc cggtacaatt
    28741 cattgctcag cgggcggcgt gggacgcaac atcgcccaca atctggcgct gttaggccgt
    28801 gacgtccatt tgctttcagt gattggcgat gacttttatg gcgaaatgct cctggaagaa
    28861 acgcgccgcg ccggcgtgaa tgtctccggc tgcgttcgtt tacatggtca aagcacatcg
    28921 acgtatctgg caattgccaa tcgagacgat gaaaccgtgc tggcgattaa cgatacccat
    28981 ctgctggaac agttatcgcc gcaattattg aacgggtcgc gcgatttact tcgtcatgcg
    29041 ggcgtggtac tggctgattg caacctgaca gccgaggcgc tggaatgggt ctttaccctc
    29101 gctggtgaga tcccggtgtt tgtcgatacc gtttcagaat tcaaagcggg caaaatcaaa
    29161 cactggctgg cgcatattca caccctgaaa cccactttac cggagctgga aattttatgg
    29221 ggacaggcga tcaccagcga tgctgaccgt aatgctgcag tgaatgcgtt gcatcagcaa
    29281 ggtgttcagc aactgtttgt ttatttgccc gatgagtcag tttattgcag cgaaaaggat
    29341 ggagaacaat ttttgctgac cgcaccagcg catacgacag tagacagttt tggtgctgac
    29401 gatggtttta tggcgggcct ggtatatagc tttctggaag gaaacaattt ccgtgacagc
    29461 gcccgttttg cgatggcctg cgcggcaatt tcacgcgcca gcggcagctt aaacaaccct
    29521 accctgtctg ccgataacgc gctttcatta gtgccaatgg tgtaacaatg ttgccggatt
    29581 cgacgcttga ccgcatctta tccggctcat aaatcgcaca ctacgctaaa ccaataaaga
    29641 acccggcaat ggtggcactc atcaggttag aaagtgtcgc cgccgccagc gcgcgtaaac
    29701 caagctgggc gatttccggc gcacggtgtg gcgcaaccgc agaaaacgcc cccaccacca
    29761 ccccgataga accaaagtta gcgaaaccgc acaacgcgaa ggaaataatc gccacggttt
    29821 tagcatccag agtgccagcc gtttgcagat agggtgagaa attgagataa gcgacaaatt
    29881 catttattgc cagtttctgt ccaatcaaac tcccggcaag attcgcatca ctccagtcaa
    29941 cacccattac ccacgccagt ggggccagca gataacctac aatggactcc agcgaggcat
    30001 gttcaaaacc aaaccagccg ccaacgccgc cgataatacc gttaattaac gcaatgatgg
    30061 cgacgaatgc catcactact gtcgccacac ctgcggcgat tttcagcccg gtcattgccc
    30121 ctgtcgcggc ggcttcaata atgctttttg gcggtgtttc ggtgaaagag aggttattaa
    30181 aagaaacctg cgaagattcc gtagccgggc ttagcaggcg ggcaaacaag atccccccgg
    30241 ggatcgccat taacgatgcc gccagcaaat attccacagg cacgcccagt gcggcataac
    30301 caatcattgt cgaaccagca atcgaggcca tgccgctaca aatcgctgta aataattcat
    30361 tgcgattcag ccgatcgata aagggcttca caatcgccgg aatttcgttt tgcccgagga
    30421 aaatggtagt gaccgcgacg aatgactcaa tcttgctgat atttaatgct ttctggaata
    30481 taccgccgag aatgcgaatt aaaatcccca tcacaccgat gtagtagaga atactcacca
    30541 gtgcagtgac gaagataatt gccggtaata ccctgaaacc aaagataaat cctgcgccat
    30601 caaataaggt gtccattttc ggtccgacca gagaaccgaa gataaatgcg ctacccgcgt
    30661 cgctgtacgc catcacttta tgcacaccaa aagcgacttt ttcagcgacc caacgccctg
    30721 gcggtaacca aagcataatg ccgccaatca cgacctgtaa cactaacgcc gcgccaacgg
    30781 tacgcaggct gatcttcttc ttgtttactg acagtaaaaa cgcaatcgtc agcaatacca
    30841 ccattcccag aacacttctc atgacatcca tagtgattat ctcttcatgt catattgggc
    30901 gacggctttc tgcccatcag aaatagccag gcctgcatct tgcgataccg gcccggcttg
    30961 ttatcaacac ttaatgggtt ttgatgtagc cgcgcacgca ctcttcgact aagccccaga
    31021 accagtcgct atcaatagtg atgccgactt tggtattagc gggcttgcca agaacgccca
    31081 gctcgtcgca gacggtacgc ccataacaag ggccactgtt gacgtccact tcgacgtaca
    31141 tctcctgggt tttaatgcca tcagggttaa tcagataacc gatgcaggtg gcgtcgtgca
    31201 ccgggccgcc agccaggccg tagttttcga actgggtttt gagagtgaag ttcatgatgt
    31261 cgctgaacag ctctccggca gggccgcctg ccctttccat tcgtgcaatc acgtccgggg
    31321 tgcaaacggt ctggttggtg agagcgaggc ccatcatcac taatggaacg ccagaggtga
    31381 acaccacgcg tgcggcttcc gggtcagcaa agatgttgaa ctcggcagat ggcgtgaagt
    31441 tgcctgtacc gtaagcgccg cccattagca caatttcacg gattttgggc aggatcgcgg
    31501 gttgcatacg cattgccacc gcgatatttg aaagcggacc aaccggcacc agagtgatat
    31561 cgccatcgct tgccatcagg gtatcaatga tgtatttcac cgcatgagtg ctttctgcct
    31621 ggcgggtcag cggctcgaat accgggccat ccagtccggt atcaccgtga atattatcgg
    31681 caacgatttg ttgccgcata atgggttgtg gcatccccgc ataaaccgga acattaatct
    31741 ccagtttctg gcaaacattc aggccattaa ttaatgtttt atcaagcgtc tgattacccg
    31801 ctacaatagt gatgcctaat aaatctattg ccggatgttt cgccgccatc attatagcaa
    31861 tagcatcatc atgacccgga tcacaatcca gaataatttt tctcttttcc attgtttatt
    31921 tcctctgttt ccagttgcgt tattttttct acagcaaaga aatttcgatc gggccgatga
    31981 ttgaatctta acaacagcgt acgtatgcta aatatgagaa atctcatagc ggataaacat
    32041 cgtgaaagaa atccacaata atgatcttaa gcagcaattg atgagtgaat ccgcgtttaa
    32101 ggattgcttt ttaacggatg tttcagccga tacgcggctg tttcattttt tagcgcgtga
    32161 ctacattgtg caggaagggc aacagccgtc ctggctgttt tacctgacgc gaggccgcgc
    32221 caggctttac gccacgctag ctaatggtcg cgtgtcgctg atcgatttct ttgccgcccc
    32281 ctgttttatt ggtgagattg agttaatcga taaagaccat gaaccgcgtg cggtacaggc
    32341 tattgaagag tgttggtgcc ttgcgctccc tatgaaacat taccgtccgc tgttattaaa
    32401 cgacacccta tttttacgaa aactttgcgt caccttaagt cataaaaatt atcgtaatat
    32461 tgtttcttta actcagaatc aatcatttcc gttagtgaat cgcctggcag catttatatt
    32521 actctcacaa gaaggtgatc tttatcacga aaagcatacg caagcggcag agtatttagg
    32581 cgtttcttat cgacatcttt tatatgttct cgcgcaattc attcacgacg gtttattaat
    32641 taaaagcaag aaaggatatc taattaaaaa cagaaagcag ttgtcagggc tggcactgga
    32701 gatggacccg gagaataaat tctccgggat gatgcagtaa aaattatttg caatagcgcg
    32761 attgccggat gcaacgcttg atgcgtttta tccggtctac aaattgagca ttacgccaga
    32821 ccaataaaga acccggcaat ggtcgcgctc atcaggttgg aaagcgttgc tgctgccagc
    32881 gcccgtaaac caagctgagc gatttccggt gcacgttttg gcgaaatagc cgaaaatgcc
    32941 ccaacgacaa caccgataga accaaagtta gcaaaaccac aaagcgcaaa ggagataatc
    33001 gcaatggttt tcacttccag cgtgccgccc gtttgcaggt atggggagaa actcaggtaa
    33061 gcgacgaatt cgttaatcgc cagtttctgc ccaatcaggc tacccgcaag gttggcatca
    33121 ctccagtcca cacccatgat ccacgccagc ggtgccagca catagccaaa aatactttcc
    33181 agagaagcat tggcgaagcc aaaccagccg ccaattccgc cgataatgcc gttgatcagc
    33241 gcaataattg ccacaaatgc cattaccacc gtcgccacac cagcggcgat tttcagcccg
    33301 gtcatcgcac cgctcgccgc tgcttcgata aagctttttg gcggcgtttc gctgaacgac
    33361 agattttcaa acgtgacctg cgaaagctcg gttgctggac taagaatacg tgcaaacaaa
    33421 ataccgccgg ggatcgccat cagcgatgcc gccaacaggt agtcaattgg tacgcccatt
    33481 ccggcataac caatcatcat cgaaccagca atggacgcca tcccgctaca aatagccgta
    33541 aacaactcgt ttcgattcat gcgatcgata aacggtttaa cgatcgccgg gatctcattt
    33601 tgcccgagga aaatagtggt gactgcgaca aaagattcga ttttgctgat gttgagggct
    33661 ttctggaaaa tgctgccaag gatgcgaatc agcagcccca tcacgccaat gtagtacagc
    33721 agactaatga gcgcagtgac gaagataatc gccggaagta cgcgaaaggc gaagataaaa
    33781 cccgcaccgt caaagaggac atccattttc ggcccgacca gcgaaccaaa aatgaaggcg
    33841 ctaccagcat cactgtaaga catcacttta tgaacgccta atgccgcctg ttctacagcc
    33901 cactttcccg gcggaaaata gagcatgatg ccaccaagag caatttgcag cagtagtgcg
    33961 gctgcaaccg tacgcaaact gatgctcttt ttattcactg acaacagaaa tgctattgcc
    34021 agtaacacca ccatccccac aacacttctc attatgtcca taatgatttt cccttcatgc
    34081 cagtaaaccc ggcgtcagcg ccaggtttcg gtatgtttga tgagtacggg cgacggcttt
    34141 ctgcccgtca gaaattaacc cgcgagacgc tgatattctt tggcaatttc gctcgccaga
    34201 atggcgttgt tgaacacaag ctggatgttg gatttcaggc tgtcaccgcc ggtcagctca
    34261 gcaacgcgcg ccagcaggaa tggcgtactt tctttaccaa taacaccctg cgcttcagct
    34321 tcagctaccg cctgatcgat caccgcattg atagtgtgtt ctggcatcgc aaactgttcc
    34381 gggatcgggt tcgctaccac gaggccaccg ttcagcccgc tttgccattt caccgccatt
    34441 gcacgggcaa tttcactggc gctgtcgaga cgaatgctga cgtcaaacgg gctggtacgg
    34501 cagaaaaacg caggcagcgc tttagtctga tagccaatta acggcacacc gaaggtttct
    34561 aaatactcag tggttaatcc taaatcgaga atagatttcg ccccggcaca aacaacggtg
    34621 acattagtat ttgccagttc ttgcaaatcg gcagaaatat cgaaggtatg ttccgcccct
    34681 cgatgaacac caccaattcc gccggtggca aatactttaa ttccggcaag cgccgcaata
    34741 atcatcgttg aagccacggt ggttgcacca ttttttcctg cggcaacgac aaaaggtaaa
    34801 tcgcgacgac taactttagt cacgttatgc ccttcacgac ccagtaattc aatttcttct
    34861 ttgcttaaac ccactttcat cacgccgcca ataatggcga tagtggcagg tactgcgccc
    34921 tgtttacgaa tagtttcttc tacttcaatt gcggtctggg cattttgtgg gaacggcatc
    34981 ccgtgagaaa taatggtcga ttccagcgcc acaaccggtt ttttgttttt taaagcgtcc
    35041 tgcacttccg gggaaatttg taataattca ggggaaattt ttaattcaga cattctgcgt
    35101 tctccactaa cgatataacg ttggcaatcg ataaatcggg gttattggtg tattcacagg
    35161 agagcgccat tgacgaacat ccctgtgcga aacgaacaga ttcggcaaac ggcattccgt
    35221 ctacccaaca cgaagcaagt cccgccatca tggcatcgcc cgctccggta acattaataa
    35281 cattggtttt gatcggcgca gaccagccac tttcaccgct gatatcgctg taataaacgc
    35341 cgtcgccgcc catgctcaat accagtcggt tcaggccatg ttgatggaac caggcagcaa
    35401 cttttgccac atcttcacgc cctgacagcg caatcccact cagggtttcc gcttcaaggc
    35461 ggtttggctt gagagtatgg atttgactta atcgctcgcg tactttgacg catttccacg
    35521 cggaaaccgg atcgacaaat acgggtacgt tggcagcatt atccagaatc catgccagtg
    35581 cctcttcact gatattacag tccgcgacaa tgacctttgc cctctgaata aattcacggt
    35641 gctgtgcgag atattcagct gtaatagcgt tgctaatatt catgtcattt atagcaacca
    35701 gcatttcacc ggtattatcg agtaatgata aatagctcga cgtattttct cccggcacaa
    35761 tcaggcattt atcgacataa acgccagatt gattggtttg cgttagcagc gattggccat
    35821 aaaaatcact gcctacggcg ctcagtagcc aggctttatt acccagcaac gccaggtttt
    35881 gtgcaatatt gcgccctacc ccaccaggcg taaattttat tttacctgga tttgaatccg
    35941 cataatttaa tgattcatgt gaatatccgg cgacatcaat attcgccgag cctataatta
    36001 cgacataatc cttttcgcgc atagcacgtc ctctggtaaa aaattaccaa ccctgtcaat
    36061 ccacaaggat atagatactg caaagacggt ttaaacatgt gttcatattt aaacatgtgc
    36121 tcatagtatg catgttttac gtaaagtaaa ttgccgccag agaggcaaag cgggcagatt
    36181 tgagcaggct cacaaaatgc gattaaatgc ctgaattaca aaagcgagga atttgtgacg
    36241 ggatgcacag aacatgaaca ctaaaaaagg gcagaaaaga atctgccctt gaggaaataa
    36301 ccttagtgtt tgatgaacgt ttcaggtttc tactgaaaga taaaaccatt taaattcata
    36361 aaattacatt cacaaaccgg tcattctctt tgcgctcatc caatgatacg gggcagtaaa
    36421 tttctgccct gtaacacacc ttttattacg cggctttcgc tactgcgtcc acttccggac
    36481 gtttcaggaa ggcataggcc aaacccgcca ccagcgtacc ggcaataatt gctaccaggt
    36541 aacccagtac cggcgtaata gcgccaggga tcagcagaac aaacagacca ccgtgcggtg
    36601 ccatcagttt cgcaccaatc gccattgaga ttgcgccagt cagcgcccca ccaacgatac
    36661 aacacggcag cacacgcatc ggatcacgag cagcaaaagg aattgcacct tccgaaataa
    36721 agcacagacc aagaaccaga gcggctttgc caccttcctg ctgcgctttg tcaaatttgc
    36781 gacgcgccac cattgttgcc agacccattg ccagcggtgg caccatacct gccgccataa
    36841 tcgccgccat cgggccatag gtttgagtac tcagcagacc cacaccgaat gcgtacgctg
    36901 ctttatttac cggaccgccc atgtcagtac acatcatgcc accgaggatc gcccccagca
    36961 gaaccgcatt cgcagtcccc atggtctgca gccagtgagt caacccttcg agaatgccag
    37021 caaccggttt accgatcagg tagatcatcg ccagaccgac caccagactg gaaattagcg
    37081 gaatgatcag gatcggtttc agcgcctcca tactctgtgg cagtttcagt tgcgtactga
    37141 ttaacttcgc aatgtaacca gccaggaagc ccgcaataat accgccaatg aagccagaac
    37201 cggtgctgac cgccagcata ccgccaatca gacccggcgt gagacccgga cgatcggcaa
    37261 tggagaaggc aatataacct gccagtaccg gcaccatcag cgcaaaggct gaaccaccac
    37321 caatctgcat cagcgccgct gccaacgtac ccggctcttt aaacgcttcg ataccaaaag
    37381 caaaagaaag cgcgatacac agaccacctg caacgaccat cggcagcatg taagagacgc
    37441 ccgtcagcaa gtgacggtat gcgcctgcac tctcgttctt accttcagag gtcgccgttt
    37501 gagctttgcc cgccggttca tacggcgttg cttcagcaac cgctttatcc agttcctgcg
    37561 cggttttctt cagcgccaga ccggtagagg tgcgatacat cggtttacca gcaaatttcg
    37621 ccagatccac ttcgatatct gccgccacaa tcaccagatc cgctgcggct acttcttcgg
    37681 gagtgattgc attacccgcg ccaacagaac cacgggtttc aactttcacc caccagccac
    37741 gttttttcgc ttcggtttca atggcttcag ccgccataaa ggtgtgtgct acgccagtcg
    37801 ggcaagcagt caccgcaact acgcgtttcg gaccgctggc ggcaaccggt gctgtcgcag
    37861 cgaccggcgc agtgtaaggt ttcgcatggc ctttggcttc actcaggaac agctcagggt
    37921 gcgcaactgc ccgggaaata tcgcccagcc agacattttt accgttcagc gcgctgtcat
    37981 tcgggatgga atcaccgaga acaatcgcca tttctgcgtc gttcggattg tcgatgattt
    38041 ccagttttgc ttttcgcgcc gccgcgccca gcagggtctt cgccatatag gcgcgtgcct
    38101 gaccgagatt agcgtcaata atcagcagcg ttttcattat gcctctcctg ctgtcagtta
    38161 aaaggttgta agtcgacgcg cgccatcatt gcggccaact gcggacgatc ggtaataccc
    38221 acattgcttt gacttaccgc cagggctgca acagcagtcg ccagacgcag tgtgtgttca
    38281 ctggattcac gcattagcaa gccataaatc aggccaccaa ccatagaatc ccctgcgcca
    38341 acggtgctta cgacatcgac tgacggtggt ttggcgatcc attcgccgga ggcattaacc
    38401 caaagcgcgc cttcggcacc cagtgaaata acgacatgcg caataccttg ttcacgcagc
    38461 gcatgcgcag cttcaatcac atctttcatt tcaggcagtt tacggcctgc ccagatttcc
    38521 agctcgcggc ggttaggttt caccagccac ggtgccgctt tcaaacctgc tactaacgct
    38581 tcacggctac tatcaaagat aatgcaaggg cactgactac gcaggcgagt catccagtcg
    38641 gtgaacgctt ccgggctgac gcctgacggt aagcttccgc tgacacagac catatcgaac
    38701 tgaccgagcc agctcagaga atcagtcaca aagcgttccc agtcggcggg ggtgacttca
    38761 aaacccgaga agttgaagtc ggtcacttcg ccgtcttttt ccgtcagctt aacgttaatt
    38821 cgggtgcgcc cctgtacaac ctggaaacgg ttggcaatgc ccagctcgct gaacagttgc
    38881 tgaaaaccat cctgattgtc tttaccgagg aagccgccaa cggtgacatc aattcccagg
    38941 tcttttaata ctttggccac gttgatgcct ttacccgccg catgcagacc ggtggttttc
    39001 accaggttca cttcgccgcg ttcaatttcc gggcagaaac caacaaggtc ataagccgga
    39061 ttaagggtga tagtagcaac acgtctgctc attatgcgcc ctccccaaga ccagcagcga
    39121 tagcgtcgcc gattgctttc agcgcctgtt cagcatctgc accctgggcg gtaaagcgta
    39181 ggcgatgacc tttcttaacg ccaagtgcca caactttcat cagactacgt ccgtttgccg
    39241 gtttgccggt gccatcaagg tttgtcacgg taatatcact gttaaattgt ttaatggtgt
    39301 tgaccagcat ggtacctgga cgagcatgca ggccgtgttc attgcgcacc acaaactccg
    39361 cgcttaacac gtcgtcggtc ggcgcatcat cgctggtcag cagcgccagc aacgttgccg
    39421 catccgcttt cagcaagcgg tcagctttat tgtcgagcaa caaatcagcg agacgcttaa
    39481 gaaccgcaat gggctgatcg tcattcatcg ccacactcac cagcatggct gccgtttcgc
    39541 cgtccacatc aaaagcattt gccgcacggc ttaccgcaat tgcgctacgc agattgcctt
    39601 cggcgctatc gctcagccag ataccctgtc cgaggttcag cggttgttca ttgatggctt
    39661 tggtgacgaa agtggcgtca actgcccccg cctctttcag acgcgcagcg ttcagcgcct
    39721 gaagagtcag cagatcgctg gcgacgatat ccagcgtcag catttcgttg tcgagcttca
    39781 gctgctcact ctgcttttcg cccatcagca atgcgcgaag ttcttctgct gttgttgctg
    39841 acttcagttg ttcagcaacg gaatcatcgc tcagtacgtg ggtcagctgg cgtagcaggc
    39901 ccagatgctc atccgagctg gcagcaatac cgatcgctac gtacgctacc tgaccgtcac
    39961 cccaggtgac gccttccggg aactgaaata cctgaacgcc ggttttcagc acctgatcgc
    40021 gggtgtcggt agtgccgtgt ggaatagcaa taccattgcc gaggaacgtt gaggtttgct
    40081 gttcgcgcgc cagcatgcca ttgacgtagc cttctgctac attaccggcc tgcaccagcg
    40141 ccgcagcgac ctggcgaatc gcctcttctt tgtctccggc cttttcgccc ggatggatgt
    40201 cctgtacgga taactggaac atagttctcc tctcttgctg aattgaaacg attcagcttc
    40261 tatgagaaaa aaagcgccaa cctggcttag ggttaaagac aagatcgcgc tgaaacgttt
    40321 caagaaagca taatacttct gtttcagcac gcaaggaaag ccgtaaagtt atgagcaaaa
    40381 gtttgatgtg ctgcacaatt ttttgtcatt tttcctaatt gctgatggga aaatccgtta
    40441 tcagcaattt catttcagca gcgtcagcag ccaatcagca acagtgaaat ggcattttga
    40501 ttattcgtgg cgcttttgat ttcgctgtct gtttattttc tgacaagcag cgtaaactcc
    40561 gcgtcttcct cttccagtga tcgaccagca tgcataactc ccccgcagtc tccagcgcga
    40621 aatcgtttga cctgacctcg acggcgtttt taatcgttgc ctttctcacc ggtattgcgg
    40681 gcgctctgca aaccccgaca ctcagtattt ttcttaccga tgaagtacat gcccgtccgg
    40741 cgatggtggg attcttcttt accggcagcg ctgtcattgg ggttctggtg agtcagtttc
    40801 tcgccgggcg ctctgataag cgcggcgatc gcaaatcgct gattgtcttt tgctgcctgt
    40861 taggcgtgct ggcctgcacc ctttttgcct ggaatcgcaa ctactttgtt ttgctattcg
    40921 ttggcgtctt tcttagcagc tttggctcga ccgctaaccc gcaaatgttt gcccttgccc
    40981 gtgaacatgc cgacaaaacc ggacgtgagg cggtgatgtt cagctctttt ttacgcgctc
    41041 aggtttcact ggcatgggtc attggcccac cgctggctta tgccttagcg atgggtttca
    41101 gctttacggt aatgtatctg agcgcagcgg tagcatttat tgtttgcggc gtgatggtgt
    41161 ggctgttttt accgtcgatg caaaaagagc ttccgctggc gaccggcacg gttgaagcgc
    41221 cgcgccgtaa ccgtcgcgat acgctgctgc tgtttgtcat ttgtacattg atgtggggct
    41281 cgaacagcct gtacatcatc aacatgccgc tatttattat caacgaactc catcttcccg
    41341 agaaactggc cggtgtgatg atggggaccg ccgccgggct ggaaatcccg accatgttga
    41401 ttgccggata tttcgccaaa cgtctgggta agcgtttctt aatgcgcgtt gctgccgtgg
    41461 gtggcgtctg tttttacgca ggaatgctga tggcgcattc tcctgtcatt ctgttgggct
    41521 tgcagctgct aaatgctatt tttattggca ttctgggcgg tatcgggatg ctctattttc
    41581 aggatctgat gcccggtcag gcaggttcag ccaccacgct ctataccaac acgtcgcgcg
    41641 tgggctggat catcgcggga tcagtggcgg gcatcgtcgc cgagatctgg aattatcacg
    41701 ctgtgttctg gtttgcgatg gtgatgatta tcgccactct gttttgctta ctgcggatta
    41761 aagatgttta aggcgcggtc agcatttcca gatcgataag ccaggtcatt gcctgctgac
    41821 gcgagttacc gcacatttca gcgctggctt gtaagcctgc acagactttc ggccgcagtg
    41881 gcgaggtgaa aattttacag cgttgctgtt catcaagctg aatgcagggc gtattggcgg
    41941 gcttgccatc tggcataccg ggaatagggc tggaaattga aggggcagtg caacacgccc
    42001 cgcaacccgg acggcattcc atacgttgtt ctcttaaggt taaccaccgg attgatgagc
    42061 gcgcacagta ccaccttttt tgcaccagca aaagtgcgaa taccacttgc ccgaaaggct
    42121 cgtcgcgagt actttgtcgc gatatttttg acattttcga ctacaggaat ttttcgatgc
    42181 caagagcgaa cgaaattaaa aaaggtatgg tactgaatta caacggcaaa ctgctgctgg
    42241 ttaaggacat tgatattcag tcgcccactg cccgcggtgc cgctacgctg tacaaaatgc
    42301 gtttttctga tgtccgtacc gggctgaaag tagaagagcg tttcaaagga gatgatatcg
    42361 ttgacaccgt gacgctgacc cgccgttacg ttgacttctc ctatgtcgat ggcaacgaat
    42421 atgtctttat ggataaagaa gactataccc cgtatacctt caccaaagat cagattgaag
    42481 aagagttgct gtttatgcca gagggcggca tgccggacat gcaggtgctg acctgggacg
    42541 gtcaactgct ggcgcttgag ctgccgcaga ccgttgatct ggaaatcgtt gaaaccgcac
    42601 caggcatcaa aggggcatct gccagcgccc gtaacaaacc ggcgacattg agcactggtc
    42661 tggtgattca ggtaccagaa tacttaagcc cgggcgaaaa aattcgtatc catatcgaag
    42721 aacgccgtta tatggggcgt gctgactaac ttcagccgca tgcagaaaag ggatagctca
    42781 ggctgtccct tttttaattt attataccaa tcttctattt tgcgcttcct gaacacctta
    42841 cgccctcaat tttcactcgt tgattgatcg ccctcacact tcatcgcatt aacaatccag
    42901 accaatttca attgctgtca tataacttta cactgtcatt gttaattaat cgttactaag
    42961 acatgactcc tatgaataca atcgcctccg ttacgctccc gcatcatgta cacgctccac
    43021 gctacgatcg ccagcagttg caatcacgta tcgttcattt tggctttgga gcctttcacc
    43081 gcgcacatca ggcgttactg accgatcgtg tgctgaatgc ccagggcggc gactggggga
    43141 tctgtgaaat cagcttgttc agcggtgatc aactgatgag ccagctccgc gcacagaacc
    43201 atttatatac cgtgctggag aaaggggcgg acggcaatca ggcgataatt gtcggtgccg
    43261 ttcacgaatg cctgaatgca aaactggatt ccttagcggc aattattgag aaattttgcg
    43321 agccacaggt ggcaattgtt tccctgacga ttaccgaaaa aggctattgt attgacccgg
    43381 ccaccggtgc actcgacacc agcaatccgc ggattattca cgatctacaa ccccctgaag
    43441 aacctcactc cgcaccgggt attctcgtcg aagcactgaa acgccgccgt gagcgcggcc
    43501 ttacaccgtt taccgtgctc tcctgcgaca atattcccga caatggccat gtggtgaaaa
    43561 acgcggtgct gggaatggca gaaaaacgtt cgccagaact cgccgggtgg ataaaagaac
    43621 acgtcagttt tccgggaacc atggtcgacc gcattgttcc ggctgcaacc gacgaatcac
    43681 tggcagaaat cagccagcat ctgggggtaa atgatccctg cgcgattagc tgcgaaccgt
    43741 ttatccagtg ggtggtggaa gataacttcg tcgctgggcg tcctgcctgg gaagtcgcag
    43801 gtgtacaaat ggtgaatgat gtcctgccat gggaagagat gaaactgcgg atgcttaatg
    43861 gcagccactc ttttctcgct tatctgggtt acctctcagg attcgcccat atcagtgatt
    43921 gcatgcagga tcgcgcattt cgccatgccg ccagaacatt aatgctggat gagcaagcgc
    43981 cgacactgcg gattaaagat gtcgatttaa cacaatatgc ggataagtta attgcacgtt
    44041 ttgctaatcc ggcgctgaaa cataagacct ggcaaatcgc gatggatggc agccagaaat
    44101 taccgcaacg catgctggca ggtattcgca tacatctggg gcgcgaaacg gactggtcgt
    44161 tgctggcatt aggcgttgca ggctggatgc gttacgtcag cggcgttgat gatgccggaa
    44221 atgccattga tgttcgcgat ccgcttagcg ataaaattcg cgatcttgtt gcgggcagca
    44281 tcagtgagca acgcgtcacc gccctgcttt ccctgcgtga agttttcggt gatgatctgc
    44341 cagataaccc gcattttgtg caggccatcg aacaagcccg gcaacaaatc gcacaattcg
    44401 gcgcgcatca ggcgctatta aacaccctca aaatttaacg atttctgcgg ttaaagcgga
    44461 tgaagctcgc cttcgtccgc tctccccttc tcttttctgc cgtttttagc caggattaac
    44521 gctcagttaa cttaccagag tattgcggag ctgttgtgac caggaccaac ctcatcaccg
    44581 gttttctcgg cagcgggaaa accacgtcga ttcttcatct gttagcccat aaagatccca
    44641 acgaaaaatg ggcggtactg gttaatgaat ttggggaagt cggaattgat ggtgctttgc
    44701 tcgccgatag cggcgcactg ctgaaagaga tccccggcgg ctgcatgtgc tgcgttaatg
    44761 gtttacccat gcaggtaggg ttgaatacct tactgcgtca gggaaaacca gaccgcttgt
    44821 tgatagagcc gactgggctg ggccatccga aacagatcct cgatctgtta accgcaccag
    44881 tctatgaacc gtggatagat ctgcgcgcca cgttgtgcat tctcgatcca cgcctgctgc
    44941 tggacgaaaa aagcgccagc aatgaaaact tccgtgacca gctggctgcc gcagacatca
    45001 ttgtcgccaa taaatccgac cgtgcgacac ctgaaagcga gcaagcgcta cagcgctggt
    45061 ggcagcaaaa tggtggcgat cggcaattaa ttcacagtga gcatgggaaa gttgccggtc
    45121 atcttctgga tttgccgcgt cacaatttag ccgagttgcc cgccagcgcc gcgcattctc
    45181 atcagcatgt cgtgaaaaaa gggttagcag cgttaagcct gccagagcat caacgctggc
    45241 gtcgcagtct gaacagtgga caaggacatc aggcctgcgg ctggatattc gacgctgata
    45301 cggtattcga caccattggc attctggaat gggcgcgact tgcaccggtg gaacgcgtca
    45361 aaggcgtgct gcgtattccc gaagggctgg tgcgaatcaa ccgtcagggc gatgacctgc
    45421 acattgaaac gcaaaacgtt gcgccaccgg acagccgtat tgagctgatt tccagcagcg
    45481 aagctgactg gaatgccttg cagagcgcgc tgttgaagct tcgtttagcg actaccgcgt
    45541 aaggttgcct gcgtttttca gtaagataat tagagaaaat atgattaaaa atttgccgca
    45601 aatagtgttg ttgaatattg tcggcctcgc gctgtttctt tcctggtata ttcccgttaa
    45661 tcatggattc tggttgccga ttgatgcgga tattttttat ttctttaatc agaaactggt
    45721 cgaaagtaag gcctttttgt ggctggttgc attgaccaac aatcgcgctt tcgacggttg
    45781 ttcactgctg gcaatgggaa tgttgatgct gagtttctgg ctgaaagaaa acgccccagg
    45841 cagacgacgt atcgtgatta ttggtctggt catgctatta actgcagtgg tattaaacca
    45901 gctgggtcag gcattaattc ctgtaaaacg ggccagccca acattgactt ttaccgatat
    45961 taaccgcgtc agcgaactgc tctctgtccc cacgaaagat gcctcacgag atagctttcc
    46021 cggcgatcac ggcatgatgc tgcttatttt ttcggcattc atgtggcgtt atttcggcaa
    46081 agttgcaggt cttatcgccc ttattatttt tgtggttttt gcgtttccca gagtaatgat
    46141 tggcgcacac tggtttactg acatcattgt cggttcgatg accgtgatat tgatcggttt
    46201 gccctgggtg ttgctgacgc cattaagtga tcgattaatc accttttttg acaaatcact
    46261 accgggaaaa aacaaacatt tccaaaacaa ataactcaca gtacttaaca tcatcaggtt
    46321 tatttttata gtgaggataa tcctgatgat gcgcaccgtg ctttcatcca tcgaaagcaa
    46381 aaatcattct ctaagtaaat gaatggattg catgcatttc actcaatcgt actttaattg
    46441 accaatcccg cttattaact ttctgtatca ctttttctta taaaaaatca tgtaaaaccg
    46501 ctcgccaaga ccgcaccaat cgggtaatct cgaactcgtt ttgcctcggc ggtagattat
    46561 cctcacagca tataattttg tgcgttagtc cacagatttg gccttaagga attgtttcaa
    46621 catgccctgg taattagtct cgtgtcgctt ggcatttttt ttataacgat atttgtcgtt
    46681 aaggacttca agggaaaaca aacaacatgg tcaaatctca accgattttg agatatatct
    46741 tgcgcgggat ccccgcgatt gcagtagcgg ttctgctttc tgcatgtagt gcaaataaca
    46801 ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag tgaaacatca tcactgcaag
    46861 cttctcagga tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg cgaattatgg
    46921 atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg cggcagcact aaaaaaggta
    46981 tcgattgttc tggtttcgta cagcgtacat tccgtgagca atttggctta gaacttccgc
    47041 gttcgactta cgaacagcag gaaatgggta aatctgtttc ccgcagtaat ttgcgtacgg
    47101 gtgatttagt tctgttccgt gccggttcaa cgggacgcca tgtcggtatt tatatcggca
    47161 acaatcagtt tgtccatgct tccaccagca gtggtgttat tatttccagc atgaatgaac
    47221 cgtactggaa gaagcgttac aacgaagcac gccgggttct cagccgcagc taataaaccg
    47281 tttggatgca atcccttggc tatcctgacg agttaactga aagcactgtt taggcagtgc
    47341 ttttttgttt tcattcatca gagaaaatga tgtttccgcg tcttgatcca ggctatagtc
    47401 cggtcattgt tatcttttaa atgttgtcgt aatttcagga aattaacgga atcatgttca
    47461 tacgcgctcc caattctgga cgtaagctcc tgcttacctg cattgttgca ggcgtaatga
    47521 ttgcgatact ggtgagctgc cttcagtttt tagtggcctg gcataagcac gaagtcaaat
    47581 acgacacact gattaccgac gtacaaaagt atctcgatac ctattttgcc gacctgaaat
    47641 ccactactga ccggctccag ccgctgacct tagatacatg caagcaagct aaccccgaac
    47701 tgaccgcccg cgcggcgttt agcatgaatg tccgaacgtt tgtgctggtg aaagataaaa
    47761 aaacattctg ttcatctgcg accggtgaga tggacattcc actcaatgaa ttgattcctg
    47821 cgctcgacat taataaaaat gtcgatatgg cgatcttacc cggcacgccg atggtgccga
    47881 acaaacccgc aatcgtcatc tggtatcgca accctttgct gaaaaatagc ggcgtctttg
    47941 ccgctctgaa tctcaacctg acgccttctc tcttttatag ctcacggcag gaagattacg
    48001 atggcgtcgc cctcattatt ggcaatactg cgctatctac cttttcttca cgtttgatga
    48061 acgttaacga attaaccgac atgccagtcc gtgaaactaa aattgcgggc attcctctga
    48121 ccgttcggct ttatgcagat gactggacat ggaacgatgt gtggtacgca tttttactgg
    48181 gcggcatgag tggaactgtc gttggcctgc tctgctatta cctgatgagc gtacgtatgc
    48241 gccccggcag agaaatcatg accgccatca agcgcgaaca attttacgtg gcgtatcaac
    48301 cggtggtgga tacacaagct ttgcgagtaa cgggcctgga agtactgcta cgctggcggc
    48361 atcctgtcgc gggagaaatt cccccggatg ccttcattaa ctttgccgaa tcgcaaaaga
    48421 tgattgtgcc gctgactcag cacctgtttg agttaattgc ccgcgatgcc gcagaattag
    48481 aaaaagtgct gccggtaggc gtcaaatttg gcattaacat tgcgccggac catctgcaca
    48541 gcgaaagctt taaagcaaat atccagaaac tgctcacttc cctacccgca caccatttcc
    48601 agattgtgct ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc acacaactct
    48661 tcgcctggct gcactcggtc ggcgtagaaa ttgctattga tgacttcggc accgggcaca
    48721 gcgcgcttat ctatcttgag cgttttacgc tcgattatct gaaaattgac cgtggattta
    48781 tcaacgccat cggtacggaa acgatcactt cacccgtact tgacgcggtg ctgacgctgg
    48841 cgaaacgcct caatatgctg acggttgctg agggggtcga aacgccggaa caggcgcgat
    48901 ggctaagcga acgcggcgtt aatttcatgc aaggctactg gattagtcgc ccgttaccgc
    48961 tggacgattt tgtccgctgg ctgaagaaac cgtatacgcc gcagtggtaa ggtgtgctta
    49021 cgtcccttat tattcatagt gaaagcatgc cggattgcgg ctaatgatga gtaaaaggaa
    49081 atccgttgca gatgattgtg cgcatactgc tgctgtttat cgctctgttc acctttggtg
    49141 cgcaggcgca ggctatcaag gaaagctatg cctttgccgt gctgggcgaa ccccggtacg
    49201 cgtttaattt caaccatttt gattatgtga accccgccgc gccaaaaggt gggcagataa
    49261 cgttgtcagc cctcggtacc ttcgataatt tcaaccgcta tgcactgcgc ggcaacccgg
    49321 gcgcacgcac cgagcagctg tacgacacgc tatttacgac ttccgatgac gaaccaggca
    49381 gttattaccc gctgattgct gaaagcgcac gctatgctga cgattattcc tgggtggagg
    49441 tcgctattaa tcctcgcgcc cgttttcatg atggttcgcc cattactgcc cgcgatgtag
    49501 agtttacttt tcaaaaattt atgactgaag gcgtgccgca atttcgtctg gtctacaaag
    49561 gcaccaccgt caaagccatt gcgccgttaa ccgtgcgcat tgagttagct aaacccggca
    49621 aagaagatat gttgagtctg ttttcgctgc cggtatttcc agaaaagtac tggaaggatc
    49681 acaaacttag cgatccgctc gccacgcctc cgcttgccag tggtccgtac cgaattacgt
    49741 cctggaaaat ggggcaaaat attgtctatt cccgcgtgaa agattactgg gcagcaaact
    49801 taccggtaaa ccgtggacgc tggaatttcg acaccattcg ctacgattat tacctcgatg
    49861 ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg gaaaacgacg
    49921 ccaaaaactg ggccacacgc tatagcggta aaaatttcga taaaaaatac atcatcaaag
    49981 atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat atccaacgtc
    50041 cggtattcag cgatcgccgg gtccgggaag caatcaccct cgcctttgac tttgaatgga
    50101 tgaacaaggc gttgttttac aatgcctgga gtcgcacgaa cagttatttt cagaataccg
    50161 aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca ccaatgaaaa
    50221 aagatttacc gccagaagtc ttcacacaaa tctaccagcc gccggtatcg aaaggcgatg
    50281 gctacgatcg tgacaacctg ttaaaagccg acaaacttct caacgaagcg ggctgggtgc
    50341 tgaaaggtca gcaacgcgtt aatgccacta cgggtcagcc gctcagcttt gaattattgc
    50401 ttcctgcaag cagcaatagt cagtgggtat tgccgttcca gcacagcctg caacggctgg
    50461 gtatcaacat ggacattcgc aaggtggatc actcgcaaat caccaaccgc atgcgcagtc
    50521 gcgactatga catgatgccg cgcctatggc gggcgatgca gtggcccagt tccgatttac
    50581 agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc gtgcaaagcc
    50641 cggttatcga ctcgctgatc aaccaaatta ttgccgcgca gggaaataaa gaaaaattac
    50701 tgccgttagg gcgagcactg gatcgcgtat taacgtggaa ttattacatg ctgccaatgt
    50761 ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccaaccc gctgtgcgcc
    50821 ctgtttacag tctgggtatc gatacctggt ggtatgatgt taataaagcg gccaaactgc
    50881 cgtcagccag acaacaggga gagtagatgg gcgcttatct gattcgccgt ctgttgctgg
    50941 tgatcccaac gttatgggcg attatcacta tcaacttttt catcgtgcaa attgcgcctg
    51001 gcggtccggt cgaccaggcc atcgccgcca ttgagtttgg taatgccgga gtattacccg
    51061 gcgcaggcgg tgaaggcgtt cgcgccagcc atgcgcaaac gggcgtcggt aatatcagcg
    51121 acagtaatta ccgtggcgga cgcggattag atccagaagt gatcgccgag atcactcatc
    51181 gctacggttt cgataagccg atccacgaac gttacttcaa aatgctctgg gactacatcc
    51241 gctttgattt tggcgatagc ctgtttcgca gcgcctcggt gctgacgctg attaaagaca
    51301 gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat ctggtgtcga
    51361 ttccgttagg cattcgcaaa gctgtttata atgggagccg ctttgacgtc tggagtagcg
    51421 catttatcat catcggctac gccattccgg cctttttgtt tgccatcctg ctgattgtct
    51481 tcttcgcagg cggcagctat ttcgacctgt tccctctacg cggcctggtt tccgctaact
    51541 ttgattcgct gccgtggtat cagaaaatca ccgattatct gtggcatatc acgctgccgg
    51601 tgctggcgac ggtgattggt ggctttgcgg cgctgactat gctgacaaaa aactcattcc
    51661 ttgatgaagt acgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta agtgaaaaaa
    51721 atattctctg gaaacatgtg ttccgcaacg ccatgctgct ggtgattgcc ggttttccgg
    51781 cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaggtgatg ttttcactca
    51841 atggtcttgg cttactgggc tacgaagcga ccgtctcgcg cgattatcct gtaatgtttg
    51901 gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt gatatcagct
    51961 atacgctggt cgatccgcgt atagattttg agggacgtta atgtcgcgac tcaaccccgt
    52021 caatcaggcc cgttgggcgc gttttcgcca taaccgtcgc ggctactggt cgttatggat
    52081 tttcctcgtt ttgtttggtt tgagtttgtg ttctgaactt atcgccaacg ataaaccgtt
    52141 gctggtgcgt tatgacggca gttggtattt cccgttgttg aaaaactaca gcgaaagcga
    52201 ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga aacaacggct
    52261 ggaaaataat ggctgggtac tgtgggcacc gattcgcttt ggtgctacca gtatcaactt
    52321 tgctaccgat aagcccttcc cttctccccc ctcccggcaa aactggctgg gaacggatgc
    52381 caacggcggc gatgtgctgg cacgtattct ctatggcacg cggatctcgg ttctgtttgg
    52441 cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc tacaaggcta
    52501 ttacggcggt aaggtcgatc tctgggggca gcgctttatt gaagtatggt cggggatgcc
    52561 aacgctgttt ttgattattc tgctttccag cgttgtgcaa cctaacttct ggtggctgct
    52621 ggcaattact gtcttatttg gctggatgag tctggtcggc gtggtgcggg cggagttttt
    52681 acgtacccgc aatttcgact acatccgcgc ggcacaggcg cttggcgtca gcgatcgcag
    52741 tattattctg cgtcatatgt tgccgaatgc aatggtcgct accctcacct ttttaccgtt
    52801 tattttatgt agttcgatca ccaccctgac ctcactcgat ttcctcggct tcggtctgcc
    52861 gctcggttca ccgtcactcg gtgaactgct gttacaaggg aaaaataacc ttcaggctcc
    52921 gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc tgatctttat
    52981 tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg acgcaaactc
    53041 tgttagcgat tgaagatttg tcggtgggtt ttcgccatca gcaaaccgta cgtacagtag
    53101 tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg ggtgagtcag
    53161 gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc ccgccggttg
    53221 aatatctctc cggcgatatt cgttttcatg gcgaatcgct gcttcatgcc agcgaccaaa
    53281 cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaagagccg atggtgtcgt
    53341 taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc caccgcggga
    53401 tgcgtcggga agcggctcgt ggcgaaattc ttaactgcct tgatcgcgtt ggtatccgcc
    53461 aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa cgccagcggg
    53521 tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat gaaccgacca
    53581 ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa ctgcaaggcg
    53641 agttgaatat gggcatgctg tttattactc ataacctcag cattgtcaga aaactggccc
    53701 accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca aaattacgcc gctacgctat
    53761 ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg tcaggcgatc
    53821 cagtgccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt caggttgcct
    53881 tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg gtgaaaaaca
    53941 tcagttttac gctacgagcg ggtgaaacac tgggtttagt gggcgagtcc ggttccggga
    54001 aaagcacgac gggactggcg ctgctgcgac tgattaattc tcagggcagc atcatctttg
    54061 acggtcagcc actgcaaaat ttaaatcgcc gccagctgtt acctattcgt catcgcattc
    54121 aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt ttgcagatta
    54181 ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc gaacaacaag
    54241 tgatagacgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt tatccggcgg
    54301 agttctctgg tggtcagcga caacgtattg cgattgccag ggcattaatt cttaagccct
    54361 cgctgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag gcgcaaatat
    54421 tgacgctatt gaaatcattg caacaaaagc atcaactggc ctatttgttt atcagccacg
    54481 atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa ggggaagtag
    54541 tggaacaagg accgtgcgcg cgcgtgtttg ccacaccgca gcaggagtat acgcgtcagc
    54601 tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc ggcaattgcc
    54661 acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt tacaaacagg
    54721 cacggctcac cttcacactc cagcaccgaa catggaactt cgatgtcgcc taacgcctgg
    54781 cttagtttat acatcaccga ccatgcatta tcaccatccg ggctaagcaa tttaagcgcc
    54841 accaggctgt tgtcaccgca cggtccattt tgcggaatcg gttcaacctt cgaacctgcg
    54901 aaaccagcag accagcgata gttctgcggc agtcgatgga cgattgagag ttgtaatgaa
    54961 gtcacttatt ttccccggaa gcacatttac ttcacaattt gtttacatca attttaacac
    55021 atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct gcgtggcctc
    55081 tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc tatatgtacc
    55141 cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt acataaattg
    55201 attttacata aaataaacat atatcggggg aatgatagat ttgtggttga catgcatcaa
    55261 caaaggaagc cttttagctt cctcgttgtg caatagatca ccgttttttc ggccgactgg
    55321 cgtacagaca gaagagaatg gagctggttg cgcagaatgc aattgaccaa atcatcggcc
    55381 atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct atgccaaaac
    55441 ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca tcaagaatga
    55501 ccgccatcgc attggatgac accatcgaca cgcagcccac aaacgccgca acgccaacca
    55561 ccagcgacca aaatcccaga cccagcagcg cactgatgac catccacgct gccataataa
    55621 attgtatcca caaccccgag cggaacatat ttaacgcgcc aatacggcgg acgaagcggc
    55681 tattaaagat ggtcatcacg aacaggaaaa caatgtttag tgcaaagtaa taaccaaagt
    55741 tttccggcgc gacgtggtta atttcaatgt agacaaacgg tccggcgctt aagaacgaga
    55801 acatcccggc aaagctgaaa ccactggcca gcatataact caggacacgc ttatgacgga
    55861 acagcgccgc aaagttacca atagtggtac gaatgtgaaa aggctggcga cgctccggtg
    55921 gtaaggtttc tttgatcaga aagaagatca ttgccgaagc cagaatcgcc gccacggcga
    55981 ggatccagaa gatgtaatgc cagctcagcc acaccagcac ccagccgcca actatcggtg
    56041 ccatcagcgg tgcaatggtc gtcaccagca tgacaaacga catcatccgc gagaactctt
    56101 ctttcgggta gatatcacgc atcaaagcgt tgataaccac gctggccgca gccgccgcca
    56161 gcccgtggaa gaaacgcatc acaatcagct gatcgatggt ttgtgccaac gcacacgcca
    56221 ccgcggcggc ggcaaacacc agcgtaccgc caagaaccac cggcttacgc ccgaagctgt
    56281 ctgccatcgg cccgtagatt aactgcccca acgcaaagcc cagaatataa gtactgaggg
    56341 tcatctgcgt actgcccgcc ggtacgccaa actgcgctga aattaccggt agcgcgggca
    56401 gatacatatc aatcgacagc ggcatcaaca tggccagcag gccaaggata aaaacaatag
    56461 caaacgacga atgctgtcgg gtggtcacaa cgggctcctg aaagtcattg aaaagttaga
    56521 cgacgctggc aatttcttct tcagttaacg gacgatattc accgggggct aaatcagcat
    56581 ccagcgtaat gccgccaata cgttcacgat gcagctcaac cacatggtta cccacggcgg
    56641 cgaacatgcg tttcacctga tgataacgcc cttcgctgat ggtcagacga acctgcgttg
    56701 gggtaatcac ttcaagcacc gctggcttag tgagatcttt ttcgttatgc agctgcacgc
    56761 ctttagcaaa ttgctctgcc gtatcgtcag ctacgggtga ttccagcgtc accagatagg
    56821 tcttctcgca atgatggcgc ggagaagtaa tgcggtgcga ccactgacca tcatctgtca
    56881 ttagtaccag accggtggta tcgatatcca gccgccctgc ggcgtgcagt ttccacgcca
    56941 ctggttcatc tagaaaatag agcaccgttg ggtgatcagg gtcgtccgtg gagcaaacat
    57001 agccctgagg cttattgagc atgaagtaac gtggaccgtg ttgctgcgcc agcgggttgc
    57061 catcgtaagc gacatcatgt tcaggaagca gtttgaacgc tgcattacgg acgatttcgc
    57121 catcgacggt gacacgattg ccgcggattt cacgcccggc aatagcacgg ctaacgccga
    57181 gttgctgtgc gataaattta tcaagtcgca tgtgtgtgat tttgcctgta aaaacggagg
    57241 tcgggcattg acccgaaaat ctgaactgtt gtctgcccag tatagcggtc taataacttc
    57301 cctcaaggga aaaagaaccg tggcatacta ttagcagaat aatctaccta cgcgagacca
    57361 tgatttttac actccgccca tatcagcaag aagccgtgga tgccacgctc aaccattttc
    57421 gtcgtcataa aacccctgcc gttatcgtgc tgcccaccgg tgcaggtaaa agcctggtga
    57481 tagcggaact tgcgcggctg gctcgtggtc gcgtgctggt gctggcacac gttaaagaac
    57541 tggtggcgca aaaccatgca aagtatcagg cgctggggct ggaagccgat atttttgccg
    57601 ccgggctaaa gcgcaaagag agccacggta aagtggtatt tggcagcgtg cagtcggtcg
    57661 cccgtaatct tgatgccttt cagggtgaat tttcgctgtt gattgtcgat gaatgtcacc
    57721 gtattggtga cgatgaagag agccagtatc agcaaatcct cactcacctg acaaaagtga
    57781 atccccactt acgcctgctg gggctgactg ccacgccttt tcgactgggc aaaggctgga
    57841 tctaccagtt ccattatcac ggcatggtac gcggcgatga gaaagccctt ttccgtgact
    57901 gcatttatga actgccgctg cgttatatga ttaaacacgg ctatctgacg ccgccagaac
    57961 gactggatat gccagtagtg caatacgatt tcagccgctt gcaggcacag agtaacgggc
    58021 tattcagcga agccgatctc aaccgcgagc taaaaaaaca acaacgtatt accccgcaca
    58081 tcatcagcca gattatggag tttgctgcaa cgcgcaaagg ggtgatgatt ttcgccgcca
    58141 ccgttgaaca cgcaaaagag attgtgggat tactacccgc cgaagatgct gcactgatta
    58201 ctggcgacac ccccggcgct gagcgcgatg tgttaattga agattttaaa gcccagcgtt
    58261 ttcgctatct ggtcaacgtc gcggtactga ccaccggatt tgacgccccg cacgtcgatc
    58321 ttatcgccat tctgcgccct accgaatcag tgagtcttta ccaacaaatt gtcgggcgcg
    58381 gtctgcgtct cgctcctggc aagactgatt gcttaattct tgattatgcg ggtaatcctc
    58441 acgatctcta cgcgccggaa gttggtacac cgaaaggcaa aagtgacaac gttccggtac
    58501 aggttttctg ccctgcctgc ggttttgcca acaccttttg ggggaaaacg accgccgacg
    58561 ggacattgat tgaacacttt ggtcgccgct gtcagggatg gtttgaagat gacgacggtc
    58621 atcgcgaaca gtgtgacttc cgtttccgtt ttaaaaattg cccgcaatgt aacgcagaaa
    58681 atgatattgc cgctcgccgc tgccgcgaat gtgacacggt actggttgat ccggatgata
    58741 tgttaaaagc ggcgctacga ctgaaagatg cgctggtatt acgctgtagc ggcatgtctt
    58801 tgcagcatgg gcacgacgag aaaggcgaat ggttgaaaat cacctattac gatgaagacg
    58861 gcgcggatgt gagtgagcgt ttccgtctgc aaacgcccgc ccagcgaact gccttcgagc
    58921 agctttttat ccgcccgcat acgcgcacac cgggcatccc tctgcgctgg atcaccgccg
    58981 ccgatatcct cgcccagcaa gccttattgc gacacccgga ttttgtcgtt gcccgcatga
    59041 aaggtcagta ctggcaagtg cgtgaaaaag tgttcgatta cgaaggtcgt tttcgtcggg
    59101 cgcacgaatt acgcggttaa tggtagtttt gattgatgta taaggcgatt gagtatagaa
    59161 tctcgcccgc ttttgcatac gcaaagcaga tcacttacct gttgctgggt cgcctgtagc
    59221 aggattaatt taagagagaa agaaatgttt actatcaacg cagaagtacg taaagagcag
    59281 ggtaagggtg cgagccgccg cctgcgtgcc gctaacaagt tcccggcaat catctacggt
    59341 ggcaaagaag caccgctggc tatcgagctg gatcacgaca aagtcatgaa cattcaagct
    59401 aaagctgaat tctacagcga agttctgacc atcgttgttg acggtaaaga aatcaaagtt
    59461 aaagctcagg acgtacagcg tcacccgtac aaaccgaagc tgcagcacat cgacttcgtt
    59521 cgcgcttaat tgctgaataa gttgtaaaaa accccgctcc ggcggggttt tttgtatctg
    59581 cagattatgc ctgattacga tattgctatt ttttgcaggc cagataaggc gttcacgccg
    59641 catctggcat tcataacaca aaaccgatta attgccgccg gacgtccggc gctgcaattg
    59701 atcgcgcaaa ttcggcggcg tgcctttaat agtcagggta tcggtcgccg gatcccagaa
    59761 aatccgttcg cccaacagca tcgcatcaaa attaatagtc aagccaccgc cgctaccggc
    59821 aaacttcgtc aactgacgca gtgtgctacg gtctgccggg aagctctctt ccagttcgta
    59881 gcctttttca gccgcaaact ccgtgaagct cacttcgctg acgcctgcca gctctttcga
    59941 cagcgattcc agttcgatct cttcgcctgc ctgcagctgc tcattacagt agctataaac
    60001 ctgctggcgc acgttctgtc gctctgcttt atccagttgt gcttcggcag tgaaatcatc
    60061 aaccgcttgc aacagcccac ggttttgcgc tttcgcgttc aatccttcgc tggccccgag
    60121 gaaatccata aagaaatccg ccactttgcg ccctactcgc cctttcagga aagtgagata
    60181 gcgggtcgac tctggattgg tttcccattc ggtaaaatcg atacgcgcaa caatatccgc
    60241 atgattgata tcaagataat gggttgggtt gatgtcgaga ttttcgttaa cgcgcatact
    60301 gctcaggttg ctcaggactg ccaccagcag atactccacc gccagataac ggtagtggca
    60361 aaacagcacg aatccaccgt cagcaaaagg atatttcgct aattcgtcac gcaggcgacc
    60421 ggttgccgcg cggctaaatg ccagaaagtc ctcttcaccc tgacgctgta agcgcagcgt
    60481 ctgcgccagt tcactctctt cgctaaacaa gccgtaagct ttatttttgg cgctatagac
    60541 ccgatgcagt tctgctacca tctcaacgac ggtttctgtc ggttccagca atgaatcgcg
    60601 caacaccagc tcaaggttct gctcatcacg cttgataagc tggtgcagag caatctggtt
    60661 gatatccaga ctcatgataa actctccttt aagaccgggc ggtattcaac caccgccggt
    60721 taagcgacgc aataaaagat ggcggtcatt ttacagaagg cggcgtaacg agaatgattt
    60781 aacgcataaa aaagcagaaa aaaaaccgtt gctacggtaa tatgttgccc tttcatcaac
    60841 taactgattt cgatttatgc cacaaatttc ccgctacagt gatgaacagg ttgaacaatt
    60901 gctcgcagag ctgctcaacg tactggaaaa acataaggct ccgaccgacc tttccctgat
    60961 ggtgttaggc aacatggtca ctaaccttat caacaccagc attgccccgg cccaacgcca
    61021 ggcaattgcc aactcttttg cccgcgcctt acagtcctct atcaacgaag acaaagcgca
    61081 ctaagggaaa cagataacag gttatggtaa ctcatcgtca gcgctaccgt gaaaaagtct
    61141 cccagatggt cagttggggg cactggtttg cactgttcaa tattctgctt tcgctcgtca
    61201 ttggcagccg ttacctgttt atcgccgact ggccgaccac gcttgctggt cgcatttatt
    61261 cctacgtaag cattatcggc catttcagct tcctggtgtt cgccacctac ttgctgattc
    61321 tcttcccgct gacctttatc gtcggctccc agaggctgat gaggtttttg tccgtcattc
    61381 tggcaacggc gggaatgacg ctattactga tcgatagcga agtctttact cgtttccatc
    61441 tccatcttaa tcccatcgtc tggcaactgg ttattaaccc agacgaaaat gagatggcgc
    61501 gcgactggca gctgatgttc atcagcgtgc cggttatttt attgcttgaa ctggtgtttg
    61561 cgacgtggag ctggcaaaag ctgcgcagcc tgacgcgtcg tcggcgcttc gcgcgtccgc
    61621 tggccgcatt cttatttatc gcctttatcg cctcgcatgt ggtgtatatc tgggccgatg
    61681 ccaacttcta tcgccctatc accatgcagc gcgctaacct gccgctttcg tacccgatga
    61741 cggcgcgacg ttttcttgag aagcatggcc tgcttgatgc gcaggagtat caacgccgtc
    61801 ttattgagca aggtaatcca gacgccgttt ccgttcagta tccgttaagc gaactgcgct
    61861 atcgcgatat gggcaccggg cagaatgtct tgttgattac tgtcgatggc ctgaactact
    61921 cacgcttcga gaagcagatg cctgcgctgg caggttttgc tgagcaaaat atttcgttca
    61981 cgcgccatat gagctccggc aacactacag acaacggcat ctttggcctg ttctatggca
    62041 tctcgccgag ctatatggac ggcattctgt cgacccgtac gcctgcggca ttaattactg
    62101 cgcttaatca gcaaggctat cagctggggt tattctcatc agatggcttt accagcccgc
    62161 tgtatcgcca ggcattgttg tcagatttct cgatgccgag cgtacgcacc caatccgacg
    62221 agcagaccgc cacgcagtgg atcaactggc tgggacgcta cgcacaagaa gataaccgct
    62281 ggttctcgtg ggtctctttc aatggtacta acattgacga cagcaatcag caggcatttg
    62341 cacggaaata tagccgggcg gcaggcaatg tcgatgacca gatcaaccgc gtgctcaatg
    62401 cactgcgtga ttctggcaaa ctggacaata cggtggtgat tatcactgcc ggtcggggta
    62461 ttccactgag cgaagaggaa gaaacctttg actggtccca cggtcatctg caggtgccat
    62521 tagtgattca ctggccaggc acgccggcgc agcgtattaa tgcgctgact gatcataccg
    62581 atctgatgac gacgctgatg caacgcctgc tacatgtcag cacacctgcc agcgaatatt
    62641 cgcaaggtca ggatttgttc aaccctcaac gccgtcatta ctgggttacc gcagcggata
    62701 acgatacgct ggcaattacc accccgaaaa agacgctggt gctgaacaat aacggtaaat
    62761 accgcactta caacttacgt ggtgaaagag tgaaagatga aaaaccacag ttaagtttgt
    62821 tattgcaagt actgacagac gagaagcgtt ttatcgctaa ctgattaatt ataaatcagt
    62881 tagcgaaata tcttacttgc aatcggtgtg gaaaacggta gtattaacaa ccacgagtcg
    62941 gcacgtagcg cagcctggta gcgcaccgtc atggggtgtc gggggtcgga ggttcaaatc
    63001 ctctcgtgcc gaccaaaaat cccaaaaaaa ccaaccgtaa aggttggttt ttttatatct
    63061 gcatttaatt cgataaacag accgcgacac atcagggcct gtttattgtc tgttatcaga
    63121 acgtccagac cacacccgcc tgggtattcc acggcgattc tacccctgcg ccattgccat
    63181 agctgactga caaatgaccg cttaacgtcg gggtaaacga tgaccttatc ccagcctgat
    63241 aaacgccacg ggttccagcc acatcattat taaatttgcc atcgtcatta actttcacct
    63301 ggttagaatc ggcgtattcc tgacgcacgg cggctttcag ccagggttcc agcgtcgtac
    63361 cgttttgcag gtccatgtga tagcttaccg ccgttcccgc ttcagcgcgt aatatccggg
    63421 tatttcccac atccgcgcgc atgccgtttg ataacgtgta gtcctgacca tctgtggtaa
    63481 agccggtaaa ggccagatag ggtctaacac tccacaatcc gtcaacccaa cggaacccgc
    63541 tctcgacatg agcacccgcg ccgttactat tgtaatcgcc aaacgctgtt gccccattac
    63601 tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt caccactcca tcaacatagg
    63661 caccgttctg atgctcccaa ccggcataag cccccagggt atagctatcg acattgcctt
    63721 tgccgccgcg atcaaaacca atatcagaat gagagtaacc aaagaacaag ccgcgaattg
    63781 tgctgctttc ttcacgggag aaacggctat cgataccgag cgtcaggccc gtcaatgttt
    63841 gctcaaaacc agctcccgca tcagtggtca cgttgttgcg ggtgttaatt gccgaactcc
    63901 acatcgccgt atcgtaacta acgcctttta cgctaccaag acgctcacgc acggtgtcca
    63961 gttctgcatc aaataccagc ggttgtgcgg ccgccatatt cagcacatca gtggttgaag
    64021 gggtaatttg cgcgcgattc tctgccagac tccagctatg gttaccatta tccagcaagg
    64081 tatattcata cgtaccgata tcaacaacgc ctccggcatt gcccaacgta aatgcagcat
    64141 caccgccgcc cgttgttacc agtgtaaggc tatctcctgc tgccgggctg gcaccggtgt
    64201 ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt gacgttgagc tgatcgctct
    64261 gatgattcgc catatcggta cgcatataaa aattgccgtt tcccgacagg gtattggtgg
    64321 tcaacgtagt gaaattttcc gcacttgccg tcacagatga acgagtcact gactcatcca
    64381 tcataatcac atttgcgcca gacatcgcaa ggttggctaa gttaaggcgg gtgttttggt
    64441 tcgtcgtagt gatatcggca atgatggtcc atgcgctatt ttcgagcgaa acatccgctt
    64501 tgctggtatc cacggcacca tgcgttctta aagaagcgcc ctcagaaacc actaccttac
    64561 ccaccaacat tgcgggtgca taatttattt gcggcttcat aacctcaaga atgccatcat
    64621 ttcctctgac tgacacgtta accattgtgc cagcccagac gttagcgcgg ccattgttga
    64681 taaccatatt ttcagagatg gctttagagg aatacgttat acttccattc tgatctgatc
    64741 gaccgagatc atataccgca tttgcctgca ctctcgtacc agtatccttt cccagcgatt
    64801 gcatagtggc atgctcatcg aggatagtgt caatggcctg cgtgtcctcc ataacaataa
    64861 gcccggaacc atcatccagt tcataatttt ttgacacacc atcttttata ctaaattggc
    64921 ctttactgtt tgtaccactc acttccagcg cattcgttga gacaattaat tttccgcctg
    64981 cttttttatc aacgccagtt gcagtcccgc catccataac ctccagtaag ccgccactat
    65041 ctacagtagt attataagcg aagtcatttt cttcaactcg caaacttccg ccgttttcca
    65101 acagcatatt gttagcaata ccattcttga tttcgaactg accgagacgg tttgttccga
    65161 atacctccat gacccgcgtg cttgctttaa tagcaccgcc tgctttctga tctaccgcaa
    65221 tcgctaatcc cccctcttta acctccagta atcctccctt ttcgatagcg gcaaggacgg
    65281 taatcccatc tttttcaacg attaagcgcc cgcctttaat cgtgggatca ttcgctgcgc
    65341 cataaacctg tagcacacca ttattgagcg tagtctggtt tgccgcgcca cccatttgaa
    65401 caatttgata accatcattt aatactgagt attctgcggt gccattcatt tcaatgtact
    65461 gatacccgcc gttaatttca gtattactac ttataccaaa ttctttaata tgctgttctc
    65521 cgccaggatt gattttcgta ttattggcgg tcccataaac cagctggata tctttttcca
    65581 ggacaacacc atcaactgtc tcaccatact caactgttga tgccaatgct atgggtgaat
    65641 ataacgctgt agctaccaga acagatattg cggaacgaca aagagaaaca gaaccagatt
    65701 gatgcattga gctttcatcc tatgaaatta attgcagtta aaagcattgg gtacagaaaa
    65761 tacccatagc tccatacccg gagtcagttt ttaaaaactg tttaaagaaa tgcacaagta
    65821 ttgtgattga ttttttagtt gtttttcttg atgagaagct gatgcaaaat tccgtcttta
    65881 taatgaaaat gatgccaaag cgaacgacaa ggttgtagtt ttcgctacat gtccatacat
    65941 aaaatggggt aacattcacg cgcctggtag cgttaccaac gctacgctca aacataatga
    66001 ttctaataaa acctcaggag actactatgc ctgaagcaac gccttttcag gtgatgattg
    66061 tggatgatca tccacttatg cgacgcggtg ttcgtcagtt actggagctt gatcctggct
    66121 ttgaagtggt cgccgaagcg ggcgacggcg cgagtgctat cgatctggcg aatagactgg
    66181 atatcgacgt gatcttgctg gatctcaata tgaaaggtat gagtggcctg gataccctca
    66241 atgccttgcg cagggatggc gttaccgcgc aaattattat cctgaccgta tcagatgcct
    66301 ccagcgatgt ctttgcactg atagacgcag gcgcagacgg ttatctgttg aaagacagcg
    66361 acccggaagt gttgctggaa gcgattcgtg ccggagcgaa aggcagcaaa gtctttagcg
    66421 aacgcgtcaa tcagtactta catgaacgtg aaatgtttgg cgcggaagaa gatcccttca
    66481 gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct ggcacagggg ctgtcaaata
    66541 aacagattgc ctcggtgttg aatatttccg agcagacagt aaaagtacat attcgcaatc
    66601 tactgcgcaa actcaatgtc cgctcacgcg tggcagccac cattctgttc ctgcaacaac
    66661 gcggggcaca ataaaaatag cccgatggca tcaccatcgg gcctcttttt atttactctc
    66721 ctgcggcgac aaatgttgca tcgcctgcgc gatactacgt tcaatcaccg cacggcgagt
    66781 atcgttggca ggtaagagtt tcaacatcat ctcccacgcg gcaacggctt cgccaaatcg
    66841 ctgctgctca aaggcattaa acgcatacat gcttaacaca cgaatattgc tatggtccgt
    66901 tctcaccaac tggcgcagca gttcgccgcc gaggcggttg tcgttgggat cagatgaacg
    66961 tgtcaacgct tcagcataac ccagtgtggc atcgctgttt ttcggatcga ggcgatacgc
    67021 agtagcgtat gcatcggtgg cgatactggc gttacccagc gccatgccaa cgcgacccaa
    67081 cataatccaa ccttctatat ctcccggatt tttttgcagt tgagtacgca tccccagcgc
    67141 aaggcgcgac atctcttctt cgttgagcgg atcggctttc ggatccagcg ccctgtccag
    67201 caacgccgga gcctgtgccg tggcctgctg ccagattttc acctgctgat aattgccagt
    67261 ctggtagtag ctgacgccag ccacaattaa cgccaccaca atacccggca gataaacaac
    67321 atatccggca cgcttacctt ccggcacgct ttgttcagga aacgcttccg gtaccacgcg
    67381 tacccgacgc cgcgaacggg cgtatatgac ccaaccgcca ataccaatag ccactaccgg
    67441 aagcacccac agcagcacgg tcagcggcgt taacggcgga tcgtaagtga cgaagttgcc
    67501 gtaacgcgcc accatataat cgacaatctc tttcttactt ttgccttcct gcatcagttc
    67561 atacactttc tggcgcaggt cggtggcaat catcgagttg gaatcggcaa tgctgttatt
    67621 ctggcatttc gggcagcgca gttcttcagt gagctgacgg aactgttgtt cctgtgcttc
    67681 atctttaaac tgcaacacgt cgatggtcgc cagcgctgag ccggagatca tcagcatcag
    67741 cacgcccaat aaaaacctca ttgtgcggcc tccttactgt atttctccca cagcggcttg
    67801 atctcttctt cccagacgcg aggattgaga tcgcccgcat ggcgatagcg aatgatgccg
    67861 ttgccgtcaa taagaaacgt ttctggcgcg ccatagacac cgagatccag ccctaacatg
    67921 ccatcgccat caaaaaggct tagcgcgtaa ggattgccca gctctttcag ccagctgatt
    67981 gccttctggc gatcgtcttt atagttcatg ccgaccacgc ggatgccctg cgcagaaagc
    68041 tgattcagat attgatgttc cgcacggcag gtcggacacc aggtcgccca gacgttaagc
    68101 agcactggtt tgccctgagt cagcacatcc gcctgataaa actgccccgg attgtccagt
    68161 gattcgagac gaaacttcgg cacaggcttg ccaatgagcg ccgattccag attggtcgga
    68221 tcatcccctt cggcattacg cgccagctgc cacagcagcg ccgcggcaat cgccaggaag
    68281 ataatcaacg gaattaacaa tactttgcgc ttcatacagc ctccggcgca gttttttgcg
    68341 ggctcacgcg cttacgatag cgaggatcaa acagacacag cagtccgccc aacgccatca
    68401 tcagcccgcc cgcccaaatc cagcgaacaa atggtttgta gtaaagacgc acagcccacg
    68461 cgccgttttc cagctcttca ccgagcgcgg cgtacaggtc acgcgtgatg ccgccgtcaa
    68521 ttgccgcttc ggtcatcatc gacccggcag tgttgtaata acgtttttcc gcatacagca
    68581 ccgtttccgg tttaccctct cgcgttacgc cgatagtcgc cacaccgcca cgccagttcg
    68641 ggccagtcac ctctttgaca tcacggaagg tgaagcgata ttcatgaata tcgacgctat
    68701 cgccggactt catgcgcaca tcacgctcaa cgctatagtt ctggctaaag gcaatgccaa
    68761 caattgtcac tgccagcccc aggtgagccg ccaccatccc ccaataactg aaggtggttt
    68821 tcgtgccgcg tgaaatacgt agcgcagctt ccgcaattgc cagcaccgca atccagcagg
    68881 ccattgccag gccgagcacc gtcatcgcca caactttgct ttcgaacagc cacggcaaca
    68941 gcagagacag caccagcgta gagatgaagg cgataatcaa taaattgcgg attttacgtg
    69001 ggcgatcgcg cccccagcgc accagaggac cgacacccag cagtagcgca aacggcacca
    69061 tcagccaggt aaacatggtg ttgaagaacg gttcgccaat cgaaatactg cccagtccca
    69121 gttgcttgtg caccagcggc agcagcgtcc ccagcaacac caccagcatc gcggcgacca
    69181 gcaaaacgtt gttcgctaac agcaaagatt cccgcgacca cagcgcattg tttacgcgtg
    69241 agcgaacttt gtgtccacgc gcggcaaaca gcagcagcga accgccaatc accagcacca
    69301 taaaggcgag gataaacata ccgcgcgccg gatcagacgc aaacgcgtgt accgatacca
    69361 gcacgccgga acgcacgagg aaagtcccca acagacacaa cgagaaggca ctgattgcca
    69421 gcagtaatgt ccacgctttg aagctggcgc gttgttcagt gaccgccagt gagtgcatca
    69481 gcgcagtccc caccagccac ggcataaacg aggcgttttc taccggatcc cagaaccacc
    69541 agccacccca gccgagttcg taataggccc atgcggaacc gagcacgatg ccgagcgtca
    69601 ggaagatcca cgctgccagc gtccacggac gagtaaaacg cgcataagtg ctgtccagac
    69661 gcccgctcag caaagaagca atggcaaaag caaacgccac cgagaaaccc acgtacccca
    69721 tataaagcag aggcggatgg aagatcagcc ccggatcctg taacagcggg ttaagatcgc
    69781 gcccttcaat cgggaagttc ggcagcgtgc gtgagaatgg gttagaggta aagagaatga
    69841 acagcaaaaa gccgacactg accatcccca ttatcgccag tacgcgggcc acaatatcca
    69901 gcggaatacg ctgactaaaa atcgccaccg caaaggtcca gccgctcatc agcagcaccc
    69961 acagcagcag tgagccttca tgtgcgcccc aggtagccgc cacgcgatac cacaccggaa
    70021 gctgggtatt ggagttgctg gcaacatagg tgacggtaaa gtcgttgacc acgaaagcat
    70081 tgaccagcac cagaaatgcg ccagccacag acataaacag cagccaggca aacaagcggg
    70141 aagacgccat catgcgcgca tctccgcgcg ccacgcccca tagcggatac acggacagca
    70201 gcagcgcaat tcccagcgcc aggcacagca gcccgttacc aatttccggc atcatgatgc
    70261 tgggtcctta taaacactcg ccgggcgacg gtggttagct tccatcgctt tctcaacttc
    70321 tggcggcgtg tagttttcat catgtttcgc cagcacttct tttgcgagga tatgattgcc
    70381 tttttccagc tcgccctgca ccacaacgcc ctgcccttca cggaacagat ccggcaaaat
    70441 gccttcgtaa gagacatcca ctgagccttc agcgtcgtaa atggtgaagg tcactttcag
    70501 cgaattggga tcgcgctgca cactacccgg catcaccatc ccgccaacgc gcagacgctg
    70561 accgacttcc ggcatctgct gagtttcacg cttgccgtag agaatttccc ccggcgtata
    70621 aaagagatcg atattcgagc gcagcgcata tagcaccaga ccgatagtca gcgccagccc
    70681 tgccaacacg gcacaggcaa tccacaagcg gtttttacgg cgaatattca tgcagcctcc
    70741 tgctgttgcg cagcacgtaa acgcgcctca cgcgcccgct gttgcgccac accacgcaga
    70801 attgcgcgat gctgcatcac cgagtgcacg accaaaacca ccagcggaat aacggtcatc
    70861 accaccgcca gccagacaaa aaaggcgtaa ccgcccattg cgaaaaattc attccaggaa
    70921 gcaaatgcag gggtcattta cggcctcttt tcagtatcag ttcactcacc cacggacggc
    70981 gtttttccat cagcaaaatc aaattacgca tccgcatcag cgtcagcgtg gcagagagga
    71041 gcaggaagcc aaaaatcgac cagcgcagcg gcgaacgcat cgccggatcg atactttgct
    71101 gcatccgcgt tgatccctga tgcagggtgt tccaccactc cacggagtaa tgaataatcg
    71161 gcagattcac cacgccaatc agcaccagga tacctgccgc acggcccgcc agacggcggt
    71221 cgtcgaaggc gtgccacagg gcaatcacac ccacatacaa aaacagcagc accagttcag
    71281 aagtcagacg tgcatcccat acccaccagg tgccccacat cggttttccc catgcagagc
    71341 cggtaaccag ggcaataaag gtaaacacgg caccaatggg ggccatcgcc gccaccgcca
    71401 ggttggccat tttcatctgc cagacaaggc caataaacgc cgccaccgcc attgatgcat
    71461 aaatgcccat cgaccagatc gccgcaggca catgcaggta gataatgcgg tagctatttc
    71521 cctgctgata atcagccgga gcaaagccga atccccagat ccagccgacg gtaagcacga
    71581 ctacactggc aattgccagc cacggtataa accagccaca gatttgatac agccgtggtg
    71641 ggatcgccag ttgatgcagt gttttccaca tagtttcgat accagactcg aacaaaaatc
    71701 agtaatccag cgttattgaa tgctgattcg taacgctgcc gccgtcgcaa aaggacttaa
    71761 tgtcgcggtg cctgccagca acgcgcctaa aattgccaga tacccgtcaa cgggcaaatg
    71821 catagaagcc gcgtccatcg cggcggtggc aaagatgagt aatgggatag tcagcggtaa
    71881 caccagtatg ctgagcagca caccaccgcg cttaagtccc actgtcagcg ccacgcccgg
    71941 tgcaccgaga aagccaagcg taggcgttcc cagcagtagc gttaacgcca tcacttgcca
    72001 gccataaaca tccattccca gtagcattgc taccagtggc gaaaggatga gtaacggcag
    72061 accggttacc atccagtgcg ccatcacctt cgccagcaca acggcgggca agggtaacgg
    72121 caacaacatc aattgttcaa gactgccgtc ctgcaaatcg tcacggaaca gtcgttccag
    72181 cgccagcaag gatgaaagca gcgcggcaac ccagataatg cccggtgcaa tacgtgccag
    72241 cagttgcggc tccggaccga tactgagcgg aaaaagggta attacaatca ggaagaacca
    72301 cagcgggttg gcgatttcag cgctatggcg aaacgctacg cgcagctcaa gacggaaaat
    72361 gcgccagaac atcatacggc cctcgtttgc gtcagtgaaa tgcggcgaat tttactttca
    72421 gcaacgttga gcggctggtg ggtagtgaga atcacaatcc ccccctgctc tgtatgctgc
    72481 gccatacgct gggtcagacg atcgacgccg ttaacgtcaa tcgcggtaaa aggctcgtcg
    72541 aggatccata acgtggcacg ggtcagccac agacgcgcta aagcgacgcg gcgttgttgc
    72601 ccggcagaaa gttgatttac cggaatatct tcgaatccgg caagcccggc ctgcgccagg
    72661 gcttccagac attgtgcggt atcgccatcg cgatgataaa agtgcagatt ttctaacgcc
    72721 gtcagccggg ttttgatccc cggctgatgg cctatccata acaggttttg atggtagctg
    72781 tcgcgtacct gatgcaaggg ctgcccttgc cagagaacct cgcctgcgtc agggcgagac
    72841 aaccccgtca gcaaacggag aagcgttgtc ttccccgcgc cgttgctacc ggtgatttgt
    72901 acccactctc ctgcgttcag cgtaaatgac aagccactaa ataaggttcg ttcatcccgc
    72961 tcacaaagta actctctgac ttcaagcata cccacgcaat aaccctgtta aaaacctggc
    73021 tcgacttcac gcatatcggg cagcttgtgc gctatccctt tatggcaatc aatacaggtt
    73081 tgcccatctt tcaccgcctg gtcatgcatc ttcgcggcaa ccgatttctg ggcggttgta
    73141 tccatatact cgaagttgtg acagttacgg cactcctgcg agttattgtc cttcatgcgc
    73201 cgccactcat tctgtgccat cgtcagacga tgagcttcaa atttctgcgg cgtgtcaata
    73261 acgccaaaaa ttttaccata cagctcttta cttgctttga gcttgcgtat catcttcggc
    73321 acaaactcgt gcggaacgtg acaatccgga caggtcgcac ggacgccgct acggttgttg
    73381 tagtgcacgg aatccatgta ttcctgatac accgtgttac gcatttcgtg gcagctaatg
    73441 cagaactctt cggtattggc tttttccatc ccagtgttaa agccgcccca gaagacgatg
    73501 ccgccgacaa aaccgatcaa cagcagcgtc cccagcgcca gacggctggg ggtacgccac
    73561 catttccaca ggcgcttaat cagaccaggc ttacggtcag aatttcccat aataacctct
    73621 tatttcccgt aaccttttga tggggtaaag gtattcccca cgattggcgc ggtatcggcc
    73681 tgcggtacgt gacattgcag acagaaataa cgacgcggag ccacttccgc gcccactttg
    73741 ccgtcgctgt ccataaagtg agtaggactg atacgcggcg cgccagtggt gcgatagctt
    73801 tcgacaccgt ggcactgcaa gcagcggttg gtattggtcg ttacctgata accttcaacg
    73861 ctatgcggga tcattggcgg ctgattcaca tagttcagcg gcatccgatc ctgctctttt
    73921 ggcatccgaa tggccccttc ctgcgtcccg gatacttccg gcgactggct aaaatcgacg
    73981 ccattagccg cccaaaccgc gccgcttacc accagggcca gcatcgccgt ccattgacac
    74041 agcgctttct tcaggtcatg gcttttcatg atttcgctcc cgaactccat cgtgtagtta
    74101 ttgtaaatac atcctcagaa cagacatcca cgcagcgacc gcacgtcatg caatcgcggc
    74161 tggtgacctg caccgggctt tgttcatcca gcaccggggc acgtagcaca tgcggttccg
    74221 ggcaaacatg aaaacaatcc atacagcggt tacatttctg ccgatcgctg gccgcaacgg
    74281 taatcacacc tttgctaccc agcacgccat acagcgcgcc aaccggacaa atgtgcccgc
    74341 accagccgtg ttcaacgacc agtagatcaa ataaaaacag cgcgagaata agcagcgcgc
    74401 cgctgccgaa gcccattacc aggctacgcc ccatcaggga aacggggttg atccattccc
    74461 agatgagcgt gccagttaag gctgatccca ccagaatgac caccaacagc acgtagcgaa
    74521 tgtggcgagg gatcgtcgca gactgattga ggtcaaagcg cctgcgtaac cagttcgcta
    74581 agtcggtaat cgggttcagc gggcagaccc agctgcaaaa taatcgcttt cctgccaggg
    74641 cgtagagcac ggtgataatc accgcaccgg tcaacgccac cgtggcaggc agatgaccac
    74701 tggcaagact ttgcagcgtc atcagcggat cggtcagcgg aacggtgtcg aataacaggc
    74761 tactgctata gttgccgtgc aagatccaca caccaaacca cggaccgctc aaaaacatcc
    74821 ccagcacgaa gaactgacaa aggcgacgta acaccagcca acggtgactg cgccaccagc
    74881 cttttttctc cagcgcctcg cgcccggcgt cacgtttacg atttgccatt gttcccctcc
    74941 agccagccga agcggtaatg gtgacctaac tcccctttcg ccagtgacag cggtaacacc
    75001 ttgattgccg gttgttccag cacgcatact ttttcgcact taccgcaacc ggtacaggcg
    75061 tcgctgtgaa ccgtcggcag aaagcgggcg tgcttaccgg tacgcgtgtt gcgctccagc
    75121 tccagggtga tggcctcatc aattttcggg cattcgcgat aacaaacatc gcagcgcagc
    75181 ccctgaaagt tgagacagtt ttcctggtcc accagtacca ccagccccat ccgcgcgtcg
    75241 tcgatcgatt caatctcacg atccagcgca ccgctcgggc acactttggc gcacggaatg
    75301 tcctcacaca tttcgcaagg aatatcccgt gcgacaaaat atggcgtgcc cgccgacagg
    75361 ccagaggcca gcgtcgccag ttttaaggtg tcgtaagggc aagcctgaac acactgaccg
    75421 caacgcacac aggcactggc aaaggcgttc tcgtttatgg ctccgggcgg gcgcaaccgc
    75481 acgccagatg cgcgtgcggt ttgctgttgt aaccccagcg ccacacccac ggcagccagc
    75541 ccgcctgctg tgcgaacgac atcgcgcaga aagcggcggc gaccattttg aggtttcgct
    75601 gaccgggaca taatggctta caccttctcc agtttgaccg cgcacttctt gaagtccgtc
    75661 tctttcgaga gcggatcggt cgcgtccagc gttagtttgt taaccagctg tgcggcgtcg
    75721 aagaacggca tgtacaccag gccctgcggc ggacggttac gaccgcgcgt ttcgacaatc
    75781 gagatcactt cgccacggcg agaaaccact ttcactttgt cgccacggcg cagatcgcgc
    75841 gctttcgcat ccagcgggtg aataaacagg accgcttccg ggaaggcacg gtgcagttcc
    75901 ggcacacggc gagtcatact gccggtgtgc cagtgctcca ggacgcgtcc ggtagagagc
    75961 cacaggtcgt actcttcatc cggtgcttcc gccgccggtt cgaacggcag cgcgaagatc
    76021 accgctttac catccggttt accgtagaac ttatagcctt cgcccgcttt cacgtacggg
    76081 tcgttacctt cgctgtaacg ccactgcgtt tctttaccgt tcaccaccgg ccagcgcaga
    76141 ccgcgcgctt tgtggtagtc atcgaacggc gcgagatcgt gaccgtgacc gcgaccaaac
    76201 catgcgtact cttcgaacag ccctttttgc agatagaagc ccagctcgcg ggattcatcg
    76261 ttcagctgat cttccgccag ttcggatacc gggaatttgc tcacttcggg ggtggcatac
    76321 agaacttcgt acagcgtttt gccacgcagt tccggtttct tcgccagcag ctcttccggc
    76381 catacttctt cagttttgaa gcggcgggag aactggacta actgccagag gtccgatttc
    76441 gcttcgcctg gcgcctgtac ctgctgacgc cagaactgag tacggcgttc ggcgttaccg
    76501 taagccccct ctttttctac ccacattgcg gtcggcagga tcaagtcggc ggccagcgca
    76561 ctgactgtcg gatacggatc ggagacgatg atgaagttgc gcggatcgcg ccagcccggc
    76621 atacgctctt cattaatgtt cggcccggcc tgcatgttgt tggtacacat ggtccagtaa
    76681 acattcagct tgccgtcttt cagcgcacgg tcttgtgcta ccgcatgcag accgattttc
    76741 gccggaatgg tgccgctcgg gatattccac ttcttctcgc agatatcacg atgtttctcg
    76801 ttagttacca ccatgtccgc aggcagacgg tgagcaaagg tgcccacttc acgcgcagta
    76861 ccacacgcgg aaggctgccc ggtcagggag aacggaccgc aacccggctg ggaaattttg
    76921 ccggtcagca ggtgcaggtt gtagaccagg ttgttggccc acacgccacg agtatgctgg
    76981 ttgaagccca tcgtccagta ggagatgact ttcttgttcg gatcggcata cagctgcgcc
    77041 agttgttcca gctgatcttt tggtacgcca gtcatttcag cggttttttc cagcgtatac
    77101 tcagcaacga aggctttgta atcttcaaag ctcatcggtt cggaggcgtc agaacccgga
    77161 ttcttcgctg ctttttccag cggatgggtc ggacgtaaac cgtagccgat gtccgtcgcc
    77221 cctttgcgca ggttaacgtg cttgctgaag aagtcctgat ttatcgcatt gttttgaatg
    77281 atatagttgg cgatgtagtt caggatcacc aggtcagatt gcggcgtaaa gatgatgccg
    77341 ttatccgcca gctcgaagct acgatgctgg taggttgaaa gcaccgccac ggtgacgtcc
    77401 tggttagaga gacgacggtt agtgatgcgt gaccagagga tcgggtgcat ctccgccatg
    77461 tttgcgcccc acagcacaaa cgcgtcagcc tgctcgatgt cgtcatagca gcccatcggc
    77521 tcatccatac caaaggttcg cataaagcca actactgccg acgccataca gtgacgcgca
    77581 ttcgggtcga tgttgttcga acggaagccc gctttaaaca gcttggacgc ggcataacct
    77641 tcccagatag tccactgacc agaaccgaac ataccgatcg attccggccc tttttctttc
    77701 agggcggttt tgaatttctc ttccatcaca tcgaaggcct gatcccaggt gattggggta
    77761 aattcgcctt ctttgtcata tttaccgttt ttcatacgca gcagcggctg cgtcaaacgg
    77821 tctttaccgt acatgatctt gggcaggaaa tagcccttaa tgcagttcag gccacggtta
    77881 accggtgcgt ccgggtcgcc ctgacaggcc accacacgcc cctgctgcgt tccgaccaga
    77941 acgccgcaac cagtaccgca gaaacggcac ggcgctttat cccatttaat ggcttcctgc
    78001 tgaccaacaa cggcgcgggc aacgcccggc acgctgagac cggcagccgc cgcagcggcc
    78061 gcaacggcgt tagctttcat aaagctacga cgactgagtt tcatggtgtt tcctcacctt
    78121 gctcttcctg ctggtgataa accagcgaca ccgccagcac gccctctacg ttgcgtactg
    78181 actcaatggt ttggatcagc gtttcgctgt cttctgcttc caccaccaca atcaactgac
    78241 cgctcggcgc gtcgctgaca gcaacttcac agccgggaaa ggcgttcagt tgggtgctga
    78301 tgtctgaaat tcgttcgctt ttggcctgca caaccaggct gcaaacttgc cagttagtgt
    78361 gcatggagat actccgcagt tatggctgat accggacagc tggctgcaca tgctccacat
    78421 ccgttgcaaa gttggctatt aagttgcggc tggtagatcc cggagagcgt cgggcgaaag
    78481 ataattgcca ttggctcaca gctatcctga cagcggcggc attcaactga ctgatacgcc
    78541 aggcacgcgt ccccaatggt gaactgcaaa tcccaggccc tggtgtggcg cggagaaaat
    78601 agcgattcgg ggcaagcctg cgcgcaggcg taacagaagc tgcactcgtt atttttgaaa
    78661 ttaacgctcg gatagccgcc cgcgccgcgt tgcagaatgt tgttttcaca agcattaata
    78721 caggcgtcgc agcgggtgca atgggtcaga aaatgagatt catcaccgga ccagggcgga
    78781 cggataccgt tactggcttt gcgccagcga ccagtgagta tgccccgacg ggatgcatca
    78841 atcttcacat tgaccttcca tcattaacgc gctctgaaaa ttgagagcga ccaaataaac
    78901 cgcataatta ataagccatt tttatagccg ctaagatatt aaaggatgtg tcaaagatgc
    78961 ataccccgat cgaggtaaaa cctgtagcag gatcaaaaga gtggcgggaa gcgtggcaaa
    79021 aacgggcttt tgctcacatt tcaaatggtt ataaatatat ttatatagcg attaattcac
    79081 cagagatatt tctgctggtt tgctctctca tcagaattta acactaaaag agcaggtaaa
    79141 attgtctgaa tattctttaa gttattcata aagcaaatta ataaatctga tgaatatgtt
    79201 aaccttcagc gacatcatcg gtgcaaacct ataaatgaag aaggaaagca aaaaatgaag
    79261 accattctac ctgcagtatt gtttgccgct ttcgccacca cttccgcctg ggcggcagag
    79321 agcgtccagc cactggaaaa aatcgcgcct tatccacaag ctgaaaaagg catgaagcgt
    79381 caggtgattc agttaacccc gcaagaagat gaatctaccc tgaaagtaga actgttaatc
    79441 ggtcagacgc tggaagtcga ttgcaatttg catcgtctcg gcgggaagct ggaaagcaaa
    79501 acgctggaag gctggggcta tgattattat gtctttgata aagttagctc gccggtttcc
    79561 acgatgatgg cctgcccgga tggcaagaaa gagaagaaat ttgtcaccgc gtatctgggc
    79621 gatactggaa tgctgcgtta caacagcaag ctgccgatcg tggtgtatac gccagacaat
    79681 gtagatgtga agtaccgcgt ctggaaggcg gaagagaaaa ttgacaacgc ggtagttcgc
    79741 taaactgccg tgaagtgcgg caccccgtag gttggataaa gcggtcacgc cgcatccgac
    79801 atctaacgcc cgagccggtt gcctgatgcg acgctggcgc gtcttatcag catacgccac
    79861 atccggcata ccatgccgga tgtggcacat cattacaacg caatatccgc cacttctttt
    79921 tgaacgggtt gcggtttcaa ctgcggtttc ggcgtactat ctgctgcttg cggtttgtcg
    79981 tatttcagcc ccagcacttc gctggtgtac tgcaactcgc gttctgttgc cgctacatca
    80041 ccgttcagct tgcgtccata agatggaacg atcgctttca acgtagcctg ccattgcggg
    80101 ctggaaacgc gatcgccaaa tactttttcc agcagattca acataatcgg cgcggcggtt
    80161 gacgcccctg gcgatgcccc caggagcgcg gcaatggttc cttgctggtc actgaccact
    80221 tcagtaccca gacgcagtac accaccttta tcggcatcac gcttgataat ctgcacacgc
    80281 tgccccgctt gccacaaacg ccagtcctct tttttcgctt gcggatagta ctctttcaac
    80341 gcttcaaaac gatcctcttc actcaacatc acctgactca ccagatattt caccagatcg
    80401 aaattatcca gcccgacgtg catcatcggc atcacgttag aggtggtggt ggaactcatc
    80461 agatcccaca atgaaccgtt tttgaggaat ttggttgaga aggtggcaaa tggcccaaac
    80521 agcactacgc gtttaccgtc cagaacgcgg gtatcgatat gcggaaccga catcggtggt
    80581 gcgccaacgg atgctttacc gtaaaccttc gccagatggt gattaaccac gtccgggttt
    80641 tccgaaacaa ggaactgtcc gcccaccggg aaaccggcgt agtctttcgc ttccggaatc
    80701 cccgattcct gtaacagctt cagtgccgca ccgcccgcgc cgataaagac aaatttcgca
    80761 cggatgttct gtgcagtgcc atttttcaga tcggcaacgg taacggtcca ggtgttatcg
    80821 tcattacgct ttagggcgcg gacttcgctg ctgagttgca gcgagaagtt agatttcttc
    80881 tgcaaggaag caattaactg gcgggtgatc tcgccgtagt tcacatcggt accaatttcc
    80941 gtacgcgtgg ctgccacttt ctgttgcgga tcgcgccctt ccatcactaa cggtgcccac
    81001 tctttgatct gcgcgtgatc ttcagagtaa cgcataccgc gaaacagcga gctttgttgc
    81061 aacgcggcgt aacgggcgcg caggaaattg acgttatcct cgccccagac aaagctcata
    81121 tgcggaacgg tattgataaa tgaacgcgga gtacgcagca cgccgcgctc gacctggtgc
    81181 gcccagaact ggcgggaaat ctgaaatgct tcgttaatgg cgactgcttt ttcaatactg
    81241 atgctgccat cggcgttttg cggggtgtag ttcagttcca tcagtgcaga atgcccggtt
    81301 ccggcgttat tccagccgtt cgaactctcc tgcgcgacac cctccaggcg ctccaccatg
    81361 gtcatcgacc attcaggctc cagctcgcgt aaataggtcc ccaacgtggc gctcataatg
    81421 ccgccgccaa tcaacagtac atcagtttcc tgctcctcgg acgctttcgc ttttgccgcc
    81481 atcgaaacgg cattaagccc cacggccatc gagaagagca tggcagtcac ttttttcatc
    81541 ttgttaatgc cttactttta gtcgctttat tgcaggtgag atttgcgcgg catcaacggt
    81601 aaca
//
LOCUS       DAZFBR010000008        80234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFBR010000008 DAZFBR010000000
VERSION     DAZFBR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.008"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(62..2491)
                     /gene="hrpB"
                     /locus_tag="ACHJZ2_000667"
     CDS             complement(62..2491)
                     /gene="hrpB"
                     /locus_tag="ACHJZ2_000667"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO4566836.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            2565..3095
                     /gene="thpR"
                     /locus_tag="ACHJZ2_000668"
     CDS             2565..3095
                     /gene="thpR"
                     /locus_tag="ACHJZ2_000668"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO4566837.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            3110..3814
                     /gene="sfsA"
                     /locus_tag="ACHJZ2_000669"
     CDS             3110..3814
                     /gene="sfsA"
                     /locus_tag="ACHJZ2_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO4566838.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            3992..4447
                     /gene="dksA"
                     /locus_tag="ACHJZ2_000670"
     CDS             3992..4447
                     /gene="dksA"
                     /locus_tag="ACHJZ2_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO4566839.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            4514..5410
                     /gene="gluQRS"
                     /locus_tag="ACHJZ2_000671"
     CDS             4514..5410
                     /gene="gluQRS"
                     /locus_tag="ACHJZ2_000671"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO4566840.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            5470..6867
                     /gene="pcnB"
                     /locus_tag="ACHJZ2_000672"
     CDS             5470..6867
                     /gene="pcnB"
                     /locus_tag="ACHJZ2_000672"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO4566841.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            6864..7346
                     /gene="folK"
                     /locus_tag="ACHJZ2_000673"
     CDS             6864..7346
                     /gene="folK"
                     /locus_tag="ACHJZ2_000673"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO4566842.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIWY
                     "
     gene            7627..8223
                     /locus_tag="ACHJZ2_000674"
     CDS             7627..8223
                     /locus_tag="ACHJZ2_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4566843.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            8310..9035
                     /locus_tag="ACHJZ2_000675"
     CDS             8310..9035
                     /locus_tag="ACHJZ2_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO4566844.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            9077..11677
                     /locus_tag="ACHJZ2_000676"
     CDS             9077..11677
                     /locus_tag="ACHJZ2_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO4566845.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            11691..12239
                     /locus_tag="ACHJZ2_000677"
     CDS             11691..12239
                     /locus_tag="ACHJZ2_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4566846.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            12254..12859
                     /locus_tag="ACHJZ2_000678"
     CDS             12254..12859
                     /locus_tag="ACHJZ2_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4566847.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            12880..13470
                     /locus_tag="ACHJZ2_000679"
     CDS             12880..13470
                     /locus_tag="ACHJZ2_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO4566848.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            13482..14591
                     /locus_tag="ACHJZ2_000680"
     CDS             13482..14591
                     /locus_tag="ACHJZ2_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4566849.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            14726..15520
                     /gene="panB"
                     /locus_tag="ACHJZ2_000681"
     CDS             14726..15520
                     /gene="panB"
                     /locus_tag="ACHJZ2_000681"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO4566850.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            15532..16383
                     /gene="panC"
                     /locus_tag="ACHJZ2_000682"
     CDS             15532..16383
                     /gene="panC"
                     /locus_tag="ACHJZ2_000682"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO4566851.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(16682..17578)
                     /gene="rpnC"
                     /locus_tag="ACHJZ2_000683"
     CDS             complement(16682..17578)
                     /gene="rpnC"
                     /locus_tag="ACHJZ2_000683"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO4566852.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            17852..18232
                     /gene="panD"
                     /locus_tag="ACHJZ2_000684"
     CDS             17852..18232
                     /gene="panD"
                     /locus_tag="ACHJZ2_000684"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO4566853.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(18236..19465)
                     /gene="yadE"
                     /locus_tag="ACHJZ2_000685"
     CDS             complement(18236..19465)
                     /gene="yadE"
                     /locus_tag="ACHJZ2_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO4566854.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(19529..19969)
                     /gene="yadI"
                     /locus_tag="ACHJZ2_000686"
     CDS             complement(19529..19969)
                     /gene="yadI"
                     /locus_tag="ACHJZ2_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4566855.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(20074..20844)
                     /gene="yadH"
                     /locus_tag="ACHJZ2_000687"
     CDS             complement(20074..20844)
                     /gene="yadH"
                     /locus_tag="ACHJZ2_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4566856.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(20841..21767)
                     /gene="yadG"
                     /locus_tag="ACHJZ2_000688"
     CDS             complement(20841..21767)
                     /gene="yadG"
                     /locus_tag="ACHJZ2_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4566857.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            21876..22538
                     /gene="can"
                     /locus_tag="ACHJZ2_000689"
     CDS             21876..22538
                     /gene="can"
                     /locus_tag="ACHJZ2_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO4566858.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(22579..23115)
                     /gene="hpt"
                     /locus_tag="ACHJZ2_000690"
     CDS             complement(22579..23115)
                     /gene="hpt"
                     /locus_tag="ACHJZ2_000690"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO4566859.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            23321..25711
                     /gene="gcd"
                     /locus_tag="ACHJZ2_000691"
     CDS             23321..25711
                     /gene="gcd"
                     /locus_tag="ACHJZ2_000691"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO4566860.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(25913..27463)
                     /gene="cueO"
                     /locus_tag="ACHJZ2_000692"
     CDS             complement(25913..27463)
                     /gene="cueO"
                     /locus_tag="ACHJZ2_000692"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO4566861.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            27629..27976
                     /gene="yacC"
                     /locus_tag="ACHJZ2_000693"
     CDS             27629..27976
                     /gene="yacC"
                     /locus_tag="ACHJZ2_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO4566862.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            28082..28948
                     /gene="speE"
                     /locus_tag="ACHJZ2_000694"
     CDS             28082..28948
                     /gene="speE"
                     /locus_tag="ACHJZ2_000694"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO4566863.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLNHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            28964..29758
                     /gene="speD"
                     /locus_tag="ACHJZ2_000695"
     CDS             28964..29758
                     /gene="speD"
                     /locus_tag="ACHJZ2_000695"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO4566864.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(29796..30158)
                     /gene="yacL"
                     /locus_tag="ACHJZ2_000696"
     CDS             complement(29796..30158)
                     /gene="yacL"
                     /locus_tag="ACHJZ2_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO4566865.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(30334..32931)
                     /gene="acnB"
                     /locus_tag="ACHJZ2_000697"
     CDS             complement(30334..32931)
                     /gene="acnB"
                     /locus_tag="ACHJZ2_000697"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO4566866.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            33286..35139
                     /gene="yacH"
                     /locus_tag="ACHJZ2_000698"
     CDS             33286..35139
                     /gene="yacH"
                     /locus_tag="ACHJZ2_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO4566867.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(35210..36634)
                     /gene="lpdA"
                     /locus_tag="ACHJZ2_000699"
     CDS             complement(35210..36634)
                     /gene="lpdA"
                     /locus_tag="ACHJZ2_000699"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO4566868.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(36842..38734)
                     /gene="aceF"
                     /locus_tag="ACHJZ2_000700"
     CDS             complement(36842..38734)
                     /gene="aceF"
                     /locus_tag="ACHJZ2_000700"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO4566869.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(38749..41412)
                     /gene="aceE"
                     /locus_tag="ACHJZ2_000701"
     CDS             complement(38749..41412)
                     /gene="aceE"
                     /locus_tag="ACHJZ2_000701"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO4566870.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(41573..42337)
                     /gene="pdhR"
                     /locus_tag="ACHJZ2_000702"
     CDS             complement(41573..42337)
                     /gene="pdhR"
                     /locus_tag="ACHJZ2_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO4566871.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            42878..44251
                     /gene="aroP"
                     /locus_tag="ACHJZ2_000703"
     CDS             42878..44251
                     /gene="aroP"
                     /locus_tag="ACHJZ2_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO4566872.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(44294..45148)
                     /gene="ampE"
                     /locus_tag="ACHJZ2_000704"
     CDS             complement(44294..45148)
                     /gene="ampE"
                     /locus_tag="ACHJZ2_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO4566873.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(45145..45696)
                     /gene="ampD"
                     /locus_tag="ACHJZ2_000705"
     CDS             complement(45145..45696)
                     /gene="ampD"
                     /locus_tag="ACHJZ2_000705"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO4566874.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            45784..46677
                     /gene="nadC"
                     /locus_tag="ACHJZ2_000706"
     CDS             45784..46677
                     /gene="nadC"
                     /locus_tag="ACHJZ2_000706"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO4566875.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            46880..47320
                     /gene="ppdD"
                     /locus_tag="ACHJZ2_000707"
     CDS             46880..47320
                     /gene="ppdD"
                     /locus_tag="ACHJZ2_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO4566876.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            47330..48715
                     /gene="gspE"
                     /locus_tag="ACHJZ2_000708"
     CDS             47330..48715
                     /gene="gspE"
                     /locus_tag="ACHJZ2_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO4566877.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            48705..49907
                     /gene="hofC"
                     /locus_tag="ACHJZ2_000709"
     CDS             48705..49907
                     /gene="hofC"
                     /locus_tag="ACHJZ2_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO4566878.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(49942..50985)
                     /gene="guaC"
                     /locus_tag="ACHJZ2_000710"
     CDS             complement(49942..50985)
                     /gene="guaC"
                     /locus_tag="ACHJZ2_000710"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO4566879.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(51141..51185)
                     /locus_tag="ACHJZ2_000711"
     CDS             complement(51141..51185)
                     /locus_tag="ACHJZ2_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566880.1"
                     /translation="MIIKTLKMSARKRN"
     gene            51210..51830
                     /gene="coaE"
                     /locus_tag="ACHJZ2_000712"
     CDS             51210..51830
                     /gene="coaE"
                     /locus_tag="ACHJZ2_000712"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO4566881.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            51830..52573
                     /gene="zapD"
                     /locus_tag="ACHJZ2_000713"
     CDS             51830..52573
                     /gene="zapD"
                     /locus_tag="ACHJZ2_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO4566882.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            52583..52780
                     /gene="yacG"
                     /locus_tag="ACHJZ2_000714"
     CDS             52583..52780
                     /gene="yacG"
                     /locus_tag="ACHJZ2_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO4566883.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(52871..53269)
                     /gene="mutT"
                     /locus_tag="ACHJZ2_000715"
     CDS             complement(52871..53269)
                     /gene="mutT"
                     /locus_tag="ACHJZ2_000715"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO4566884.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(53329..56034)
                     /gene="secA"
                     /locus_tag="ACHJZ2_000716"
     CDS             complement(53329..56034)
                     /gene="secA"
                     /locus_tag="ACHJZ2_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO4566885.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(56096..56608)
                     /gene="secM"
                     /locus_tag="ACHJZ2_000717"
     CDS             complement(56096..56608)
                     /gene="secM"
                     /locus_tag="ACHJZ2_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO4566886.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(56839..57756)
                     /gene="lpxC"
                     /locus_tag="ACHJZ2_000718"
     CDS             complement(56839..57756)
                     /gene="lpxC"
                     /locus_tag="ACHJZ2_000718"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO4566887.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(57857..59008)
                     /gene="ftsZ"
                     /locus_tag="ACHJZ2_000719"
     CDS             complement(57857..59008)
                     /gene="ftsZ"
                     /locus_tag="ACHJZ2_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO4566888.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(59069..60331)
                     /gene="ftsA"
                     /locus_tag="ACHJZ2_000720"
     CDS             complement(59069..60331)
                     /gene="ftsA"
                     /locus_tag="ACHJZ2_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO4566889.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(60328..61158)
                     /gene="ftsQ"
                     /locus_tag="ACHJZ2_000721"
     CDS             complement(60328..61158)
                     /gene="ftsQ"
                     /locus_tag="ACHJZ2_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO4566890.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(61160..62080)
                     /gene="ddlB"
                     /locus_tag="ACHJZ2_000722"
     CDS             complement(61160..62080)
                     /gene="ddlB"
                     /locus_tag="ACHJZ2_000722"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4566891.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(62073..63548)
                     /gene="murC"
                     /locus_tag="ACHJZ2_000723"
     CDS             complement(62073..63548)
                     /gene="murC"
                     /locus_tag="ACHJZ2_000723"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO4566892.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(63602..64669)
                     /gene="murG"
                     /locus_tag="ACHJZ2_000724"
     CDS             complement(63602..64669)
                     /gene="murG"
                     /locus_tag="ACHJZ2_000724"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO4566893.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(64666..65910)
                     /gene="ftsW"
                     /locus_tag="ACHJZ2_000725"
     CDS             complement(64666..65910)
                     /gene="ftsW"
                     /locus_tag="ACHJZ2_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO4566894.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(65910..67226)
                     /gene="murD"
                     /locus_tag="ACHJZ2_000726"
     CDS             complement(65910..67226)
                     /gene="murD"
                     /locus_tag="ACHJZ2_000726"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO4566895.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKFSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(67229..68311)
                     /gene="mraY"
                     /locus_tag="ACHJZ2_000727"
     CDS             complement(67229..68311)
                     /gene="mraY"
                     /locus_tag="ACHJZ2_000727"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO4566896.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(68305..69663)
                     /gene="murF"
                     /locus_tag="ACHJZ2_000728"
     CDS             complement(68305..69663)
                     /gene="murF"
                     /locus_tag="ACHJZ2_000728"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO4566897.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(69660..71147)
                     /gene="murE"
                     /locus_tag="ACHJZ2_000729"
     CDS             complement(69660..71147)
                     /gene="murE"
                     /locus_tag="ACHJZ2_000729"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO4566898.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(71134..72900)
                     /gene="ftsI"
                     /locus_tag="ACHJZ2_000730"
     CDS             complement(71134..72900)
                     /gene="ftsI"
                     /locus_tag="ACHJZ2_000730"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO4566899.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(72916..73281)
                     /gene="ftsL"
                     /locus_tag="ACHJZ2_000731"
     CDS             complement(72916..73281)
                     /gene="ftsL"
                     /locus_tag="ACHJZ2_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO4566900.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(73278..74219)
                     /gene="rsmH"
                     /locus_tag="ACHJZ2_000732"
     CDS             complement(73278..74219)
                     /gene="rsmH"
                     /locus_tag="ACHJZ2_000732"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO4566901.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(74221..74679)
                     /gene="mraZ"
                     /locus_tag="ACHJZ2_000733"
     CDS             complement(74221..74679)
                     /gene="mraZ"
                     /locus_tag="ACHJZ2_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO4566902.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(75281..76285)
                     /gene="cra"
                     /locus_tag="ACHJZ2_000734"
     CDS             complement(75281..76285)
                     /gene="cra"
                     /locus_tag="ACHJZ2_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO4566903.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(76367..76453)
                     /locus_tag="ACHJZ2_000735"
     CDS             complement(76367..76453)
                     /locus_tag="ACHJZ2_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO4566904.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(76465..76956)
                     /gene="ilvN"
                     /locus_tag="ACHJZ2_000736"
     CDS             complement(76465..76956)
                     /gene="ilvN"
                     /locus_tag="ACHJZ2_000736"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4566905.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(76959..78683)
                     /gene="ilvI"
                     /locus_tag="ACHJZ2_000737"
     CDS             complement(76959..78683)
                     /gene="ilvI"
                     /locus_tag="ACHJZ2_000737"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO4566906.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIG
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(79001..79945)
                     /gene="leuO"
                     /locus_tag="ACHJZ2_000738"
     CDS             complement(79001..79945)
                     /gene="leuO"
                     /locus_tag="ACHJZ2_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO4566907.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT        19566 a        21827 c        19659 g        19182 t
ORIGIN      
        1 aacgtgcgcg ctggtgccct caccccggcc ctctcccaca gggagaggga gaaaaccaac
       61 cttacgaata ctttttcgtc cgtcgcgtcg gtgccgtgtt tgccgggtcg tccggccaga
      121 cgtgtttggg ataacgccct ttcatctcct tttgcacctc acgatacgcc cctttccaga
      181 aggcccccaa atcacgcgtg atttgcagcg gcctttgggc gggtgaaagc aattccagca
      241 ccagcggcac gcgcccctgg gcgatcgtcg gattagtggc ctcaccaaac atctcctgca
      301 ttctcaccgc cagcgcgggc gggttatctt catgataacg aatggcgatc cggcttcccg
      361 tcggcacagt gtaatgcgca ggcaattcac tatccagacg ttgctgcatt ccccaatcaa
      421 gtaatccgcg tagtgcctga taaatgtcga gtgatttcag gccgcgtagt gaatgtacac
      481 cggccatatg tggcagcagc cacgtttcca gcgtcgccaa caaactttca tcatcaaccg
      541 ctggccagtc atattctggc aaccactttc cggcgcataa caaacgcaag cgtagctgtt
      601 ccgcttccgc cgtccagttg agcacgctta aacctttatc acggatgccg ttaagcatcg
      661 cctgatgcaa ctcgtcttct gacggtttcg ccaacggctg cactttcacc gtcaactgac
      721 cgatttgcag ccgacgccag gctttcagcg taccttgcgc gtcatcccat tccacggtgt
      781 cagattgctg caccagctgc ggacagcgtt gtactaattc atcaatatcg accggtaacg
      841 ccagtaaaat ccgcgcatcc ggcgaggcgc tgccctgcaa taataacggc gcgatcaacc
      901 attcgtggcg acttagcgcg tcgtcggcat caagcatcgc ccccatgccg tttgccagtt
      961 gatagcgtcc atcttgccca cggcggcgag caatgcgatc ggcaaacgcc ccggcaagta
     1021 gcggcgcgat aagcgaactg tctgcctcac cgccgcgtac gtttaagcgt tttaacagtt
     1081 gctgactacg ttgctgccag gctggttgat tgcgcgaaaa cgccacgccc aggtcactat
     1141 tgcccatccg tggcggctct tcgagaatgg cggcaatttt tgccgcggta gcagcttcgt
     1201 cgtcgctctt tgcgctaacc agcattgccg ctaaacgcgg atcgttaccc agcgctgcca
     1261 ttttttgccc ttgcgcacta agccgttcac cgtccagcgc ccccagcatt tgtaacagac
     1321 gtttcgcggc cagtagattc accgttggcg gttgatccag ccagctcatc tgcgccggat
     1381 cgctgcatcc ccattgcagt aattccatca gcaaaccgga aagatcgctt tgtaagatct
     1441 ccggttcact ttgcgcggta gcgcgttctg cttgttcttt ggcgattaaa tgcaggcaga
     1501 tacccggctc cagacgcccg gcgcgcccgg cacgctgcgt catcgacgcc tgactgattc
     1561 gctgagtaat caaccgcgta agccctgttc gcggatcaaa acgcgccacc ctttcctggg
     1621 cacaatccac caccagacga ataccttcaa tggttaaact ggtttcagca atattggtcg
     1681 ccagcaccac tttgcgcatc ccttgcggtg ccgggaggat cgcttttcgc tgatcgttca
     1741 gcgacaacgc gccatacagc gggcagagca atacatcact gccgatgcgc gaagccagtt
     1801 gttcctgcac acgctgaatt tctccgacgc caggtaaaaa taacagtaat gatccgcttt
     1861 cctgacgcag catttcagcg gtggctaccg caacggcatc gtcaaaacgc tgatgcgtgg
     1921 gcagcggtaa ataacggcgt tcaaccggaa acgagcgccc ttctgagatg acgacgggcg
     1981 cttctggcag catttgctgc aagcggtcgt tgtccagcgt agccgacata atcagcagtt
     2041 taaggtcatc acgcagacct tgttgcacat cgagtaacag cgccaacgcc aaatccgcct
     2101 gcaagctacg ctcatgaaat tcatcaagga tcaccaatcc aacaccgctc agttccgggt
     2161 cacgctggat catccgcgtc agcacgcctt cggtaactac ttccaggcgg gtattcggcc
     2221 cgacgcagtt ttgcgcacgc atccggtagc caacggtatc gcctggcttt tcgttaagca
     2281 gctccgccag ccgttgcgcg acgttacgcg ccgccagacg ccgtggctcc aggaggataa
     2341 ttttcccgtt aatgccggga tgcgccagca gttgcagcgg cagccaggtt gatttcccgg
     2401 ccccggtcgg cgcacttaat aatacctgcg gcgcataatc gagagcggta agtaattcag
     2461 gtaacacggc agcaacgggc aacgacgaca caaaacgctc cagagggtta acattcttcg
     2521 cgccacattg tagcatcgcg gtaattcata accgagtgcc tcacatgtct gacccgcaac
     2581 gtctgttctt tgctatcgac ttacctgcag aaatccgcga acagattatc cactggcgcg
     2641 ccacacactt cccacttgag gcgggacgtc cggtcgccgc cgataatttg catctgactc
     2701 tggcattttt aggcgaagtg agcgcagaga aagagaaggc gctttctctt ttagccggac
     2761 ggattcgtca acctggtttc acactcacgc ttgatgacgc cggccaatgg ctgcgttcgc
     2821 gtgtggtgtg gttagggatg cgtcagccgc cacgcggctt aatccagctg gcgaatatgc
     2881 tccgttcaca ggctgcccgc agcggttgtt ttcaaagcaa tcgtccgttt catccacata
     2941 ttaccttatt gcgcgacgcc agcgaggcgg tgacaatccc gccgccaggt tttaactggt
     3001 catatgcggt gacggagttc accctttacg cctcctcgtt tgcccgtgga cgcacacgct
     3061 acacgccgct aaaacgctgg gtgctaacgc aataacaagg attgtcgcaa tggaattttc
     3121 tccccctcta cagcgcgcga cgctaattca gcgttacaaa cgttttttag ccgatgtgat
     3181 cacacccgat ggtcgcgaat taacgctaca ctgcccgaat acgggtgcga tgaccggttg
     3241 tgcaacgcct ggcgataccg tctggtattc gacttcagac aacaccaaac ggaaataccc
     3301 acacacctgg gaattaactc aaagccagag cggcgcattt atttgcgtca acacgctttg
     3361 ggctaacagg ttgacgaaag aggctatcct taatgaatca atttcagaac tgtcaggcta
     3421 tagctcgctg aaaagcgaag taaaatacgg cgcagaacgc agccgtattg actttatgtt
     3481 gcaggcggat tcgcgtccag actgctatat tgaagtgaaa tcggttacgt tagcggagaa
     3541 cgaacaggga tattttcccg atgcggtcac tgaacgaggt cagaaacacc ttcgggagtt
     3601 gatgagcgta gcggctgaag gccagcgtgc ggttatcttt ttcgccgtgc tgcattcagc
     3661 cattacacgg ttttcacccg cgcgccacat cgatgagaaa tacgcgcaac tattgtcaga
     3721 agctcaacag aggggggtag aaattctggc ttacaaagcg gaaatttctg ctgaaggcat
     3781 ggctcttaaa aaatcactgc cggttacatt gtagtaaagt aagtaactgg ttaatttaca
     3841 ttctggtcgc gtgcgcaaat acgcttttcc tcacacagtt gtcaagtgtt acgtttagat
     3901 aattgctatc cggaaaagca tctgctattt atagcggcct catttttccc ccgaacatgg
     3961 ggatcgatag tgcgtgttaa ggagaagcaa catgcaagaa gggcaaaacc gtaaaacatc
     4021 gtccctgagt attctcgcca tcgctggggt ggaaccatat caggagaagc cgggcgaaga
     4081 gtatatgaat gaagcccagc tggcgcactt ccgtcgtatt ctggaagcat ggcgtaatca
     4141 actcagggat gaagtcgatc gcaccgttac acatatgcag gatgaagcag ccaatttccc
     4201 ggacccggta gaccgtgcag cccaggaaga agagttcagc ctcgaactgc gtaaccgcga
     4261 tcgcgagcgt aagctgatca aaaagatcga gaagacgctg aaaaaagtgg aagacgaaga
     4321 tttcggctac tgtgaatcct gcggtgttga aattggtatt cgccgtctgg aagcgcgccc
     4381 gacagccgat ctgtgcatcg actgcaaaac gctggctgaa attcgcgaaa aacagatggc
     4441 tggctaatta cagccgttcc atcacgttta ccacaggcgg gaaatgctcc cgccttattt
     4501 tttgttcaaa gagatgacag acacacagta tattggccgc ttcgccccct ctccttccgg
     4561 cgagcttcat tttggttctc tgattgctgc gcttggcagc tatttgcagg ctcgcgcccg
     4621 gcacggtcgc tggctggtac gcattgaaga tatcgacccg cctcgtgaag ttcccggtgc
     4681 cgcagaaact atcctgcgcc agctggaaca ttacggtctg cactgggacg gcgatgttct
     4741 ctggcaatcg caacgtcacc acgcctatcg tgaagcactc gcctggttac atgaacaagg
     4801 actaagttat tactgcacct gcacgcgtgc gcgtattcaa agcattggcg gtatttacga
     4861 cggtcattgc cgggtgttgc atcatggacc agacaacgcc gcagtgcgta tccgccagca
     4921 gcatccggtc acgcaattta ctgaccagct gcgcggcatt attcacgccg acgaaaaact
     4981 ggcacgggaa gattttatta ttcatcgccg tgatggtttg tttgcctata acctggcggt
     5041 ggtggtggat gatcacttcc agggcgtgag cgaaattgtc cgcggggcag atttgatcga
     5101 accgacagtg cggcaaatct cgctgtacca gctttttggc tggaaagtgc cagattacat
     5161 tcatctgccg ctggcgctta atccacaagg cgctaaactt tccaagcaga atcatgcgcc
     5221 tgcgttgccg aaaggcgatc cacgcccggt actaatcgcg gcacttcaat ttctggggca
     5281 gcaggcagaa gcacactggc aggatttcag cgtcgagcaa atccttcagt cagccgtcaa
     5341 aaactggcgg cttaccgcag tgcctgagtc ggcaattgta aattcaacat tctcaaatgc
     5401 gtcatgctga gctatgatta gccgctattt ttttgtcctg aatgatgttt gacactaccg
     5461 aggtgtacta tttttacccg agtcgctaat ttttgccgca aggtgctaag ccgcgaggaa
     5521 agcgaggctg aacaggcagt cgcccgtcca caggtgacgg tgatcccgcg tgagcagcat
     5581 gctatttccc gcaaagatat cagtgaaaat gccctgaagg taatgtacag gctcaataaa
     5641 gcgggatacg aagcctggct ggttggcggc ggcgtgcgcg acctgttact cggcaaaaag
     5701 ccgaaagatt ttgacgtaac cactaacgcc acgcctgagc aggtgcgcaa actgttccgt
     5761 aactgccgcc tggtgggtcg ccgtttccgt ctggctcatg tgatgtttgg cccggagatt
     5821 atcgaagttg cgaccttccg tggacaccac gaaggtaacg tcagcgaccg cacgacctcc
     5881 caacgcgggc aaaacggcat gttgctgcgc gacaacattt tcggctccat cgaagaagac
     5941 gcccagcgcc gcgatttcac tatcaacagc ctgtattaca gcgtagcgga ttttaccgtc
     6001 cgtgattacg ttggcggtat gaaggatctg aaggacggtg ttatccgtct gattggtaac
     6061 ccggaaacgc gctaccgtga agatccggta cgtatgctgc gcgcagtacg ttttgccgcc
     6121 aaattgggta tgcgcatcag cccggaaacc gcagaaccga tccctcgcct cgctaccctg
     6181 ctgaacgata tcccaccggc acgcctgttt gaagaatcgc ttaaactgct acaagcgggc
     6241 tacggttacg aaacctataa gctgttgtgt gaatatcatc tgttccagcc gctgttcccg
     6301 accattaccc gctacttcac agaaaatggc gacagcccga tggagcggat cattgaacag
     6361 gtgctgaaga ataccgatac gcgtatccat aacgatatgc gcgtgaaccc ggcgttcctg
     6421 tttgccgcca tgttctggta cccactgctg gagacggcac agaagatcgc ccaggaaagc
     6481 ggcctgacct atcacgacgc tttcgcgctg gcgatgaacg acgtgctgga cgaagcctgc
     6541 cgttcactgg caatcccgaa acgtctgacg acgttaaccc gcgatatctg gcagttgcag
     6601 ttgcgtatgt cccgacgtca gggtaaacgc gcatggaaat tgctggagca tcctaagttc
     6661 cgtgcggctt atgacctgtt ggccttgcga gctgaagttg agcgtaacgc tgaactgcag
     6721 cgtctggtga aatggtgggg tgagttccag gtttccgcgc caccagacca aaaagggatg
     6781 ctcaacgagc tggatgaaga accgtcaccg cgtcgtcgta ctcgtcgtcc acgcaaacgc
     6841 gcaccacgtc gtgagggtac cgcatgacag tggcgtatat tgccataggc agcaatctgg
     6901 cctctccgct ggagcaggtc aatgctgccc tgaaagcatt aggcgatatc cctgaaagcc
     6961 acattcttac cgtttcttcg ttttaccgca ccccaccgct ggggccgcaa gatcaacccg
     7021 attacttaaa cgcagccgtg gcgctggaaa caacacttgc gcctgaagaa ttacttaatc
     7081 acacacagca aatagagttg cagcaaggtc gcgttcgtaa agcagaacgc tggggaccgc
     7141 gcacactcga tcttgatatc atgttgtttg gtaatgaaac cattaatacc gaacgactca
     7201 ccgtcccgca ttacgatatg aagaatcgcg gctttatgct gtggccgctg tttgagattg
     7261 ccccggaact cgtgtttcct gatggggaga cattacgtga aatactcact tcgctagcag
     7321 tactacaacc tgccatctgg tattagctgt attattttat tttcttatct tactcctcac
     7381 ttcccctctt acagattaaa tatacttaat ttacatttta gtttttttta attaattcat
     7441 gaaatagcac ttcatgcaaa tagattaggc attttttgtt taaaaaacct agaatagcaa
     7501 caattcaata caccacgtaa atatattcac acccgtgata attcacccct attacatcct
     7561 ccaaaaactt tgagtaatat ccgctcgcaa tattaatttc atataatttt tgtaaggatg
     7621 catgtaatga aaaaagcact tctcgcagcc gctctggtta tggcttctgg ttccgccctg
     7681 gctgtagatg gtggtcatat cgactttaac ggtatggtac agtccggtac ctgtaaagtg
     7741 ggtgtggtag atactggtat gcatagcgtt accactgatg gcgtggttac cctggatact
     7801 gcgaatgtta ctgatacttt tgctgaagtt agcgcaactg ctgtcggttt actgccgaaa
     7861 gagttcatga tttctgttga gtgtgatcca ggtgctccga agaatgctga gttaactatg
     7921 ggttctgcaa gttacgcgaa caccagcggt accctgaata acaatatgaa catcactgtt
     7981 aacggtattg caccggctca gaacgtaaac attgcagttc ataacatgaa aaacaaagct
     8041 ggcgctgctg aaattaagca ggtccatatg aacaactctt ctgaagttca ggaactgaca
     8101 ttagacgcag aaggtaaagg ccagtacgta tttaacgcat cttacgttaa agcaccgaac
     8161 agcccggctg taactgctgg tcatgtaacc actaacgcgc tgtacaccgt tgcttataag
     8221 taattgatgg tgtgttgagc ataaggtttg ccagcaggca aaccttattt tcgcgaattt
     8281 aatctttcta cagacagcaa ggagtgctta tgtttccctt ggttaaaaaa acggttagtg
     8341 cattatttgt gagtactctg ttagccagtg caccagcatt cgctgacatt attatttccg
     8401 gaacacgtat catttataac gctgataaaa aagatgttaa cgttcgtctg gaaaataaag
     8461 ggaatcgccc cttactgatt cagaactggt tggatacggg cgatgacaat gccgatccca
     8521 gccagatcaa agtgccgttt accgcgaccc caccagtttc acgcgtggag ccaaaacgcg
     8581 gtcaaaccgt aaaagtgatg tataccggtg caacagccct gccgaaagac cgtgaaagcg
     8641 tctactggtt taacgtgctg gaagtgccgc ctaagccgaa agatgctgag gctgacaaaa
     8701 acttgttaca gctggctttc cgcacacgca tcaaactctt ttatcgcccg agtgggttac
     8761 agggagaacc ggctgaagca ccggcgaaaa tcacctggaa attaaacaac gctcagttac
     8821 aggcaaataa cccaacgcca tattacgtgt cctttaatga ggtcaagctg gaaagcggcg
     8881 gcaaaaccta caacgtcaat tccagcatgg tcacgccgtt cagccaggca agctttggcg
     8941 taaccagtct gccaggaagc gtgtcatccg gaaaagtggt ctttaaagcg atcaacgact
     9001 ttggcggcaa cattgatggt tccgcaacat tctaaaaaat aaacggattc tttttaacat
     9061 cgccaggaat gcaaccttgt accagtttac tcatcaaaaa agccgtatcc cgaaaaaaac
     9121 gctacttgcg gcctgttgtg ccctgtttta tagcagcaac ggtgctgcgg cggacaccgt
     9181 ggaatatgac agttcctttt taatgggaac tggcgcatca acgattgatg ttaaacgtta
     9241 tgctcaaggc aacccgacac cgccgggtct ctataatgtc cgcgtatttg taaacggtca
     9301 ggcgacttcc agcttagaaa ttccgtttgt ggatattggc gaaaacagtg cggcggcctg
     9361 tcttacccat aaaaacctgg cgcaacttca cattaagcaa cctgaacagc ctgtcacttt
     9421 actcgccaga gaaggtgaag aagaggattg tctggatctg gcaaagtcat acgaaaaggc
     9481 ggatgtgtgc tttgacggta gtgaccagtt tctcgatctg acgatccctc aggcctatgt
     9541 tctgaaaagc tatggcggct acgttgaccc ttctttatgg gaatcgggaa ttaacgctgc
     9601 cacactggca tataccctga acgcgtatca cacaagttca gataacgaca atagtgacag
     9661 cgtctatggc gcgttcaact caggtatcaa tttaggagcc tggcactttc gtgcgcgcgg
     9721 taactataac tggacaacag ataacggcag cgatttcgat ttccaggatc gttacttaca
     9781 gcgtgacatt ccggcaatcc gttcccagat aattatgggt gatgcctata ccaccggtga
     9841 aacgtttgac tctgtcaacg tccgtggtgt tcgcctgtac agcgacagcc gtatgctgcc
     9901 ttcggcgctg gccagttacg ctccgaccat ccgcggtgta gcaaactcca acgccaaagt
     9961 caccgtgacg caaagcggat ataaaattta tgaaaccacc gttccgcccg gtgaatttgt
    10021 tatagacgac attagccctt ccggctttgg tagcgaactg gtcgtgacca ttgaagaagc
    10081 ggatggttcc aaacgcacct ttacgcaacc cttctcgtcg gttgtacaaa tgcaacgtcc
    10141 tggtgtgggc cgttgggatt tcagcgcggg taaagtcatt gatgacagtc tgcgatccga
    10201 acccaatatg gggcaagcct cttattacta tggtctgaat aacctcttca cgggttatac
    10261 cggcattcag ttcaccgata ataactatct tgccgggctg ttaggtgtgg gtatcaacac
    10321 cagcatcggc gcctttgcgg tagacgttac ccattcccgt gctgaaattc cggatgataa
    10381 aacctaccag gggcaaagtt atcgcgtgac ctggaacaaa cttttccagg ataccgggac
    10441 atcatttaac ctcgcggcgt accgctattc cacccaggat tacctgggcc tgcatgatgc
    10501 gttagtcctc attgacgacg ccaagcattt gtctgccgat gaagacaaaa acaccatgca
    10561 gacgtactca cgtatgaaaa accagtttac cgtcagcatt aaccagccat tgaatatcgc
    10621 ctatgaagat tacggttcgc tgtttatttc cggtagctgg acgtattact gggcggcgaa
    10681 caatagccgc actgaatata atgttggtta cagtaaaagc gtttcgtggg gcagtttcag
    10741 cgtcaaccta caacgtagct ggaatgaaga cggcgagaaa gatgacgcga tgtacgtcag
    10801 cgttagcgta cctattgaga atattttagg tggcaaacgt aagtcttctg gtttccgcaa
    10861 tttaaatact cagctcaata ccgatttcga tggttcacat cagttgaatg ttaacagttc
    10921 cggtaacact gaaaacaatc tggtgaacta cagtgtcaac gcaggttata gcctcgataa
    10981 aaacgccggc gatttagcct ctgttggtgg ttatctcaac tatgaatctg ggttaggcgg
    11041 tatttccgct tcggcctcgg ccacttctga taacagccaa cagtactcca tctcaaccga
    11101 tggcggcttt gtattacaca gtggtggttt aacgttcact aacaacagtt tcagcagtaa
    11161 cgacacgctg gtgttaatca acgccctagg tgctaaaggc gcacgaatca ataacagtaa
    11221 taacgaaatc gatcgctggg gatatgccgt gacgtcctct gtcagcccat atcgtgaaaa
    11281 ccgggtaggt ctgaacattg aaacactgga aaacgatgtt gaactgaaaa gtaccagcgc
    11341 caccaccgta ccacgtagcg gctccgttgt tttgacccgt ttcgaaactg acgaggggcg
    11401 ttctgccgtg ctgaatatta ctgccgccaa tggcaaatcc attccgtttg ctgcggaggt
    11461 ttaccagggt gaggtgatga tcggcagcat gggccagggt ggccaggcat ttgtacgcgg
    11521 tattaacgac agcggggaat taatcgtgcg ctggtatgaa aacaaccaaa ccattgactg
    11581 taagttgcac taccagttcc cggcgcagcc acaaacgcag ggaagcacca acaccttatt
    11641 acttaacaat cttacctgtc aggtagcaaa tcactaatat ggaaaattct atgaaaagga
    11701 tactactaac atccgcgtta ataggcctgg gtttaccggc ggttggctct gcaacagatc
    11761 ttaatgtcga ttttacggcc accgtattag ccaccacctg cacgataact atagtggaag
    11821 atggtggtcc agcagtgacc ggtagtaacg atgaatacag cctgacaatt cctgatgttg
    11881 gtctggataa agtcgctacc gctgcgcccg aagctcaggc caactttaaa ttaaaggcct
    11941 ccgattgcag caatggttac tcaaagattt ttaccacact tacaggaaca acggtttccg
    12001 gtaagttaat tgttaatgaa gcaacctctg gcgctgctgg ggtggggatg gggattaagc
    12061 gtagagatac cgcagactcg actttcttca cacccaataa tactgacaaa tttgaatgga
    12121 gcgccgatga gaaagccagc ggtgtgcctc tgaccgtagc actgcgcgaa acaacggcgg
    12181 gagcaggacg caccggtgcg tttcaggcaa aagccacctt taacttcacc tacgaataac
    12241 agggacagcc accatgaaat taaaagtcat cgctacactg attgctactg ttgccgtggg
    12301 tgtaagcttt aacagcaatt ttgcttctgc gagtacaacg tccgcttctt taaccgtaaa
    12361 cagtaacctg actatgggta cctgcagtgc tcagataatg gataatagta ataaagtgat
    12421 caatgaagtg gtctttggca atgtttatat ttctgaactc ggtgcaaaaa gcaaagtgca
    12481 acagtttaaa attcgcttta gcaattgctc tggccttccc caaaacagcg cccaaatagt
    12541 gctggcacct aatggtatat cctgtgctgg ttctcaatcg tcatcggcgg gtttttctaa
    12601 caagtttact gacgctagcg cagcaaccag aacggctgtg gaagtatgga ctacagatac
    12661 accggaaagc aatggcagta cgcaattcca ttgtgctcaa aagataccag tgcctgtgac
    12721 gcttcccgcc gacaccacaa ctcagcctta cgattacccg ttaagtgcac ggatgaccgt
    12781 tgcggaaggt agattggtaa ccgatgtaag accgggtaat ttccgctctc ccacgacttt
    12841 cacgatcact tatcagtaat agcggataag gatattagaa tgctatgcag gcatcataaa
    12901 atcgtacact ttttagggct ggcaacagcc cttatcacgc cttttgccta ttccggccag
    12961 gatgtggatc tcaccgcaaa gatcgtgccc agcacctgtc aggttgaagt cagtaataat
    13021 ggcgttgtcg atctcggcac agtgacgctg gactattttg ccgacaatgt aaccccgaca
    13081 acggattatg cgggtggcaa aacgtttaat gtgaatgtgg tctcttgcga taacattcaa
    13141 acgacacagt cgcaaatgaa attagatttt cagccgcagg cgggatcgct tgcacaggcg
    13201 aataatcaaa tttttagtaa tgaatatgaa cagcaggcta ccggggcgaa aaacgtcggc
    13261 attgtgattt tttcggccca accgaatcag cagacattta acgtgcgagg aacggatggt
    13321 tcctcaacag caatttattc ggtagcacct ggcaatgctg ttccctcaac ctggaccttt
    13381 tattcccgta tgcagcgggt gaataatgcc cttcctcctg aatcgggaat ggtgagaagc
    13441 caggttattg ttaacgtatc ttacgaataa taaggataat tatgcaaagg aaaggcaata
    13501 aactgttgat tcagttatgc agtgtgatac tgctattttt taccacatcc tggtatgcat
    13561 tggcgaatga atgttatata gagagaaatg ctgaagggga ttatcacatg aagataagct
    13621 ctactcagct tagtctggcg tcacaaatgg tcgaggttcc gacagaaata gccgaagcta
    13681 catgggatgt aaatattcaa ctaagaggcg atgccatagg gtgtaaatct cttggggata
    13741 gtaaggcagt tcactttctt aatacagctg acccaagttt aatatccacg tacaccacaa
    13801 cgaatggcgc agcgttatta aaaacaactg ttccaggcat tgtgtattct gtcgagttat
    13861 tatgccttag ttgtggtgcc gcagatgaac ttgatttatg gctacctgca caaagtggcg
    13921 cagataactt cataccaagc acccagacga aatgggccta tgagtacagt gatcaaagtt
    13981 ggtatttacg ttttcgctta ttcataactc ctgaatttaa acccaagaat ggtgtttcca
    14041 gcggaacaac gatagcagga aagattgcgt catggtatat aggtaccaat gaccagccgt
    14101 ggatcaactt ttacattgac aatgactctt taaagttttt cgtcgatgaa ccgacctgtg
    14161 caacagttgc cctggcacaa gatcagggca acgtcagtgg caatcaggta acgcttggga
    14221 acagctatgt ttcggaagtg aaaaatgggc ttacgcggga aatccctttt tctatccgtg
    14281 ctgaatactg ttatgccagt aaaattacgg ttaagttgaa agcggcaaat aaacccagcg
    14341 atgccacact ggtgggtaaa acgactggct cggcttcagg cgtggctgta aaagtaaatt
    14401 caacttatga caatagcaaa gtattgttaa aagcagatgg tagcaacacg gttgactaca
    14461 acttcgccgc ctggtcaaac aacctgctgt ttttaccttt tacggcgcag ctggtaccgg
    14521 atggtagcgg taatgctgtc ggtgttggaa cattttcagg taacgcgacc ttctccttta
    14581 cctacgaata agtaaaacgt cattccctcc tcatcgcacc agagttctgc tctggtgcga
    14641 acatttcccc acactaataa ccattgcctc cctgttttcg gctgttagaa tgcgatcaat
    14701 ctgactttag ccatcaggat acgttatgaa accgaccacc attgcttcat tgcagaaatg
    14761 taagcaggaa aaaaaacgct tcgccaccat caccgcctac gactacagct tcgcaaaact
    14821 gtttgccgag gaagggctga acgtgatgct ggtgggcgac tcgctgggga tgaccgttca
    14881 ggggcatgaa tctacccttc ccgttaccgt tgaagatatc gcctaccaca ctaccgcagt
    14941 acgtcgtggc gcgccaaact gcctgctgtt ggctgacctg ccgtttatgg cgtatgccac
    15001 gccggaacaa gcctttgaaa acgccgcaac ggttatgcgt gccggtgcta acatggtcaa
    15061 aattgaaggc ggtgagtggc tggtcgaaac cgtaaaaatg ctgaccgaac gtgccgttcc
    15121 tgtatgtggt cacttaggtt taaccccaca gtcagtgaat attttcggtg gctacaaagt
    15181 tcaggggcgc ggcgatgaag cgagcgatcg actgcttagc gatgcattag ccttagaagc
    15241 cgctggtgca cagttgctgg tgctggaatg cgtgccggtt gaactggcaa aacgtattac
    15301 cgaagcgctg gcgatcccgg ttatcggtat tggcgcaggc aacgttaccg atgggcagat
    15361 cctcgtgatg cacgatgcgt tcggcattac cggcggtcat atccctaaat ttgccaaaaa
    15421 tttcctcgct gaaacgggcg acatccgcgc tgcggtgcgg cagtatatgg ctgaagtgga
    15481 gtccggcgtt tatccgggcg aagaacacag tttccattaa ggagtgacgt tgtgttaatt
    15541 atcgaaaccc tgccactgct gcgccagcaa attcgccgcc tgcgtatgga aggcaagcgc
    15601 gtggcgctgg tacctaccat gggcaacctg catgatggcc acatgaagct ggtcgacgaa
    15661 gccaaagccc gcgccgatgt ggtcgtcgtc agtattttcg ttaacccgat gcagttcgac
    15721 cgcccggagg acctggctcg gtacccacgc accttgcagg aagactgcga aaagctgaac
    15781 aagcgcaaag tggatctggt tttcgcgccc tcggtaaaag agatctaccc gaacggtact
    15841 gaaacccata cttacgttga cgttccaggg ctttcgacca tgctggaagg cgccagccgg
    15901 ccggggcatt ttcgcggcgt gtcgactatc gtcagcaagc tgtttaacct ggtccagccg
    15961 gacatcgcct gcttcggtga aaaggacttt cagcaactgg cgctgatccg caaaatggtt
    16021 gctgatatgg gctttgatat tgagattgtc ggtgtgccaa ttatgcgcgc caaagacggt
    16081 ctggcactga gttcccgtaa cggttatctg acggcagaac aacgcaaaat tgcgcccggt
    16141 ctgtacaaag ttttaagttc gattgccgac aaattgcagg ctggcgaacg ggatctcgat
    16201 gaaattattg ctattgccgg gcaagaactg aatgaaaaag gcttccgcgc cgatgatatt
    16261 cagattcgcg atgccgacac attgctggaa gtctctgaaa ccagcaaacg ggcagtaatt
    16321 ctggtagccg cctggcttgg cgatgctcgc ctgatcgaca acaaaatggt cgagctggcg
    16381 taatacttat actctaaata attcaagttg caagttgcag gaaggcggca agcgagtgac
    16441 aaattcgtca ggaacgaatt tgcccagccg caggctggcc ttcggtgaga gacatggatg
    16501 tctctcatta atccccagga gcttacataa gtaagtgact ggggttagcg aacgcagacg
    16561 cagcacatgc aatttgaagt atgacgagta taactggcgc tacggctgat ggcgccaatt
    16621 attaatttgc cccacgtcat ggcgatggcg tctgtaagca attattcata acgcagacaa
    16681 cttagttaat cagcctatca atctcagcaa gagataaatt tgtcatctct gcaacgtctt
    16741 cccgagacat ccctttactc agaaaacgct gggcgaattc ttgcctctcc tcctgtcttc
    16801 cctgctgaat ccccttctca atccctttct cttcaaacca ctgtgccagc gtcatcatag
    16861 acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct tgttcagtat
    16921 gaccgcgttg cagcatatag ttttgcatgg caactaactg actttcgcta gtgtaccctt
    16981 cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg cgaatatgtt
    17041 tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca tccggtgtga
    17101 tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc tccggcgagt
    17161 aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc tgataaaaga
    17221 gaatcggcac taccagcggt agtttgtcgt gatccgcctc cagatgccga tgcatggcgg
    17281 ctatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt tggtgttcaa
    17341 tcacaacatg cagataaccg ggattgccct gcatttgcac ggaatagagc acgtccgtgc
    17401 tgtgtccttt aaggctctct tcaatgaaac tccccgactc taaatgaagc gtgttgaggt
    17461 cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga gccgtctccg
    17521 catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt gcatccatct
    17581 tccctgtctt ccgtgttgtc ttgcgtgaag gcagtatcca gtcaaggcgt gctatcgtca
    17641 gccacctctt tattgtttac cgagcagcgt tcagagaaat atcaattcgt tacaggcgat
    17701 acatggcacg cttcggcgcg gtacgcaaac tgctgatgca aatgccgtca gatgagtaga
    17761 cactaaacaa aaatcgggca atactgcgtg agaatttcct caaagcaggc accgcctgct
    17821 tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc agggcaaact
    17881 ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg ccattgacca
    17941 ggattttctt gacgcagccg gtattctcga aaacgaagct attgatatct ggaatgtcac
    18001 caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga gaattatttc
    18061 tgttaacggt gcggcggccc actgcgccag tgtcggcgat attgtcatca tcgccagctt
    18121 cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aacgtcgcct attttgaagg
    18181 cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt gattgttatc
    18241 cctgcggctg gttactcacc agccgcgaca tcgtctccag cgaatccgtt cttaagatat
    18301 aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc atggttgtca
    18361 ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta aagccgccaa
    18421 acggatacga aagataccag acatgcggat taaattgcgc cagagcgcga cgtgaacgtg
    18481 caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg cgataaccat
    18541 ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa tacatcgcga atttcgttaa
    18601 gctcagaaac gctcataaat tgcagcgatt ttggttccca cttctgcggg tgacgtttga
    18661 tgcgcgaggt aacaataaac gccgtcgcct tcatgccata ttgtttcaac acaggatacg
    18721 catagcggct cactgacttg aggccatcat caaaggtaat caccaccgct cgcgcaggaa
    18781 gattgatctt attcttcacg taaccttcca gctgcaccat gctcagtgtc gcgtatcccc
    18841 tgtcgcgcag ccaggccatc tggttattga aagcgcgtac cgaggtggtc gtcgaagtat
    18901 ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataggttagc accggaaggc
    18961 cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca atacggatct
    19021 gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag cgcaaattgt
    19081 ccgccagtac cccaaatggc gcactgccca cactcggcgc gttatagacc ggcgtatctt
    19141 tccaggtaac taagttctga ttactcagcg gcttgttgag gtcacccaaa ccgtcttcaa
    19201 ctttttgtcg cccctgaacc ggctcaagat gacctttatc gataaaccct ttatcaaagc
    19261 caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt tgtccggccc
    19321 gaatattccc cacggtcacc atcttgtcac caatttgcgc ccagaccgca gcattttcga
    19381 tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac agcatcagca
    19441 ggagaataac agcttgtttg tacatgaaaa aatcaccgag ggtgaaacaa cgcttttatt
    19501 gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt ttgaggacga
    19561 aaggcggatt tttttgttgc tggtgccaaa tactactcac ctccggcgca cccagttcga
    19621 caatacgctc gcgaaactct gaactggtca tggtttcacg gcaagccatc atctgttcaa
    19681 ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg cttaataatg
    19741 aagccacgcg gtacggtggc gctccggcta tatcggttaa gaagatgaca ccctcaccag
    19801 agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagaa cttaatccgc
    19861 gccagaaatt cacggcccgg cactgaagaa gcgccccatg ttttttctcc agcacatcca
    19921 gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc gccttcctcc
    19981 ttagccgaaa atcagtctac cctgaccatt gttgataaag ggtgatagcc gtcaaaactc
    20041 ctctccccca aatccagagg agaggaaata gccttagcta cgcaaaccac gtccacgttg
    20101 gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta cgccaaaggt
    20161 agtgaccagc ggaacatcat tgataccgag gaagccgtag cggaaaccac tgatcatata
    20221 aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag tcagtgagta
    20281 aaagacccca cccaaatacg tgagtggcgt taacacaaag gttggcacca ggctgatgtc
    20341 atcgaacgtt ttggcaaaca caccgttcag caaacccgca agggagaaca acaccgccgt
    20401 gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca caaaaaacag
    20461 tgaaattgcc gtcaccagaa tgccaacaaa cagaccacgc gccacgccac cgccgacata
    20521 tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa tattacgctg
    20581 gaacttggca ccaaaaaatg atgacgcgac gttggcgtag gcgttggtga tcaccgacat
    20641 catgatcagc cccggtacga tgaactgcat gtagctgaag ccatgcatat cgccaatacg
    20701 cgaaccaatc aggttaccga aaataataaa gtaaagggtc atggtgatga ctggcggcac
    20761 cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga tgctttttag
    20821 cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac cagtgaaaca
    20881 aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat gccctgctca
    20941 cttaactgcg taaatacgct gttgatcccc tgctcacgca gcacttcaac ttccagcgtc
    21001 gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact tttcggtgcg
    21061 agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat cgaggtattt
    21121 tccaccagct caccgtgttg aataatgccg atattgcggc acagcatttc tgcttcttcc
    21181 aggtagtgtg tggtgagaat gatggtggtg cctttgtcgt ttaaatcctt caaaaagccc
    21241 cacattgagc ggcgaagttc aatatccacg cctgcagtcg gctcgtcgag aatcagtagt
    21301 ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc gccagataac
    21361 atgcgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata tttttcgctg
    21421 cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac cacaatttgc
    21481 tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa ctgacgttta
    21541 gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc ggaggtttta
    21601 tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt cggcccgaga
    21661 agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc ctgaacgccg
    21721 cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa tttttactta
    21781 ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc tatattagcc
    21841 aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat acactcatca
    21901 gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt tttgagaaac
    21961 tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt cgcgttcctg
    22021 cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat gttgctaacc
    22081 tggtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg gatgtactcg
    22141 aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa gccgcagttg
    22201 aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat atctggttca
    22261 aacatagctc attgctcgac gaaatgccgc aagagcgccg tctggatacc ttgtgtgaac
    22321 tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa tcagcgtgga
    22381 aacgcgggca gaaagttacc attcacggct gggcttatgg cattcacgac ggcttgctgc
    22441 gtgatctgga tgttactgcc accaaccgcg aaacccttga gcaacgttac cgtcacggga
    22501 tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc ggatgcaaca
    22561 catccggcaa cttcacactt actcgtccag cagaatcact ttgccgatat acggcagatg
    22621 acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg ggatcgagaa
    22681 accgataaat tctaccggga cgttcacttc acgacgggac ggtttatcca gcagcgtaca
    22741 aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg acagtgtatt
    22801 ccccgagtcg atgatatctt caacaatcag cacatccttg ccacggatat cttcatccag
    22861 atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc tggaggcggt
    22921 cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca taaacataaa
    22981 tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt aacgctcagt
    23041 aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga tcattacttc
    23101 tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc gcacgggcag
    23161 gttttggcac cgcaacaaag cgcaagacac gcagtatacc agcaaaacga ttcattcgcg
    23221 gaatctgtta ataaagctca aattgtgatg acgatcacac atgttaaaac ctataattaa
    23281 ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta acaatacagg
    23341 ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg ggctgtatct
    23401 actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc ctatcgctgg
    23461 ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgtgccg cgctttggct
    23521 atacgccgcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg gtttcgactt
    23581 ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc tgatcctgcc
    23641 gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac tggtggtggc
    23701 actgctgatt agcggtagta tcctgacctg ggccggattt aacgatccgc aggaaatcaa
    23761 cggcacctta agcgccgatg ccacacctgc ggaagctatc tcacccgttg ccgatcagga
    23821 ctggcctgcc tatggtcgca atcaggaagg tcaacgcttt tcacctctga aacaaattaa
    23881 cgccgataac gtccataatc tgaaagaagc ctgggtgttc cgtactggcg atgtgaagca
    23941 gccgaacgat ccgggcgaaa tcaccaatga agtgacgccg attaaagtgg gcgacaccct
    24001 ttacctgtgt accgctcacc agcgcctgtt tgcgctcgat gccgccagcg gcaaagagaa
    24061 atggcattac gatcctgagc tgaaaactaa cgagtctttc cagcacgtaa cctgccgtgg
    24121 cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg cggattgccc
    24181 gcgtcgtatc attcttccgg tcaatgatgg tcgcctgatt gcgattaacg ctgaaaacgg
    24241 caagctgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca atatgccaga
    24301 caccaaaccg ggtctgtatg aaccgacttc gccaccgatt atcaccgata aaaccatcgt
    24361 gatggcaggt tcagttaccg ataacttctc aacccgcgaa acgtctggcg tgatccgtgg
    24421 ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga aagatccgaa
    24481 cgcaatcccg tctgacgaac acacctttac ctttaactcg ccaaactcgt gggcaccagc
    24541 ggcctatgac gcgaagctgg atctggtgta tctgccgatg ggcgtgacca cgccggatat
    24601 ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc tggcgctgaa
    24661 tgccacaacc gggaaattgg cgtggagcta ccagaccgtt caccacgacc tgtgggatat
    24721 ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga aagtgccagt
    24781 tatttacgct ccggcgaaaa ccggcaacat ttttgtgctc gatcgtcgta atggcgaact
    24841 ggtggttccg gcaccggaaa aaccggttcc ccaaggtgct gccaaaggcg attacgttac
    24901 ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga gcggtgcgga
    24961 tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc accagatgcg
    25021 ctatgaaggc attttcacgc cgccatctga acagggcacg ctggtcttcc cgggtaacct
    25081 gggaatgttt gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg caattgccaa
    25141 cccaatggca ctgccgtttg tttcgaaact gatcccacgc ggtccgggta acccgatgga
    25201 gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac agtacggtgt
    25261 accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat gtaaacagcc
    25321 agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt ggaagaaacg
    25381 tattggtacg ccgcaggaca gtatgccgtt cccgatgcct gtaccggtgc cgttcaatat
    25441 gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt ttatcgccgc
    25501 tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac tgtggcaggg
    25561 tcgtttacca gcgggtggtc aggcgacgcc aatgacctat gaagtgaatg gtaagcagta
    25621 tgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggtg actatattgt
    25681 ggcttatgcg ctgccggatg atgtgaagta agacttgctc agattgctga caacgtgcgc
    25741 gttgttcatg ccggatgcgg cgtgaacgcc ttatccagcc tacaaaattg tgcaaaatca
    25801 atggattgca caggtaacgt aggcctgata agcgtagcgc atcaggcaga tttgcgtttg
    25861 tcatcagttt aatgcccgga gagatccggg catattcccg aatacggtct ttttataccg
    25921 taaaccctaa catcatcccc gtatcttcat gctccagcag atggcagtgc gccatataag
    25981 cacgttcttt cggtgcgtcg tgattaaact tcaccagcac ttcactgaca ttgccttcta
    26041 ccttaacggt atctttccag cccgcgcgat gagccgctgg cggtttgcca ttttctgaca
    26101 agatacggaa ctgcgtgccg tgaatatgga acggatgcag catcatgtcg cccacgccag
    26161 agataaccca acgttcgtac tgccctttcg ccgccgcaaa catcggctta ttcatatcaa
    26221 acgcctgacc gttgatttta ttggcatggt ggaaatcgaa cttcccgccg tggttcatat
    26281 gattcatatt gccgtgcccc atatggccca tcatctggct gtgatccatt ccggccatcg
    26341 cctgatcgcc atatttctcc attagcatct gcatccccat catatcgagc atcgggtcca
    26401 tagaaagttg cagcttgcgt accgtcagcc cttccagcga aggtaacgca ggcaggctac
    26461 ttaatgtgtc tggcaaagca ccggaagcac taatagcaat cggctgaatc cgcattaccg
    26521 gatgaggctt atcaaacggc gcaatcgcca tccccatctg gctgaccggc agcgtcacca
    26581 ggtcaaaggg tttgttgtcg ttaacctcca ccagcacttc aaaacgctcg cccatcagca
    26641 ccggcagctc gttcaccttc accggttcag gtagcagacc accgtcgctg gcaatcacat
    26701 aaagcgggcg attgtcgctg gtggcgaaat tgagcgagcg ggcattacag ccattgagca
    26761 aacgcaggcg cagccaacca cgcggggcag cgtgttgcgg gtagattgca ccgttggtca
    26821 gcaacgtatc gccaaaccag cccacggcgg cggtcatcac atccagttga taatcaatct
    26881 gcccgtcggc gctaaatttc ttatcctgaa cgatcaccgg aacatcatcg ataccccact
    26941 gttttggcag cattaatttc aggatctcgt catcttcaat caccaccaga ccagccagcc
    27001 ccatcgccac ctgtcgcccg gtcttgccat gttgatgtgg atggaaccag caggtagcgg
    27061 caggttgatc aacgttcaac gtcaccgagc gcttgccacc tggcggaata attccctgtg
    27121 ggccgccgtc gacttcaccc ggtacttcca gcccgtgcca gtgcaacgtc gtctcttccg
    27181 tcagttggtt atagatatca accgttaccg ctttgccgcg ctgtaatttc accgccggcc
    27241 ccagcagatt gccgttatag ccccaggtag ttgcagtttt ctcgccaaag gtagactgac
    27301 ctgcgccaat agttaactga atgcgattac gggcatcggt cgtgagcaaa tcagggatcg
    27361 gtaacgttgg gcgttccgcc gcaaagactg cacggctcca cagtggcaag gctgaagcca
    27421 cacccagtgc gacggaatat ttcaagaaat cacgacgttg catagttatt tccttattct
    27481 taagcaggcg aaacaaaatc aaacgttgag catagtcctt ccccttacgg gaaggtcaag
    27541 ccgcagacac aatttaatcg acaataatgg tcgtcgcctc gctcgcagtg tgctaacgtt
    27601 tatcttcttt aagcccctgg tagaagcaat gaagacgttt ttcagaacag tgttattcgg
    27661 cagcctgatg gccgtttgcg caaacagtta cgcgctcagt gagtctgaag ccgaagatat
    27721 ggccgattta acggcagttt ttgtctttct gaagaacgat tgtggttacc agaacttacc
    27781 taacgggcaa attcgtcgcg cactggtctt tttcgcccag caaaaccagt gggatctcag
    27841 taattacgac accttcgaca tgaaagctct cggtgaagac agctaccgcg atctcagcgg
    27901 cattggcatt cccgtcgcta aaaagtgcaa ggccctggct cgcgattcct taagcctgct
    27961 tgcctacgtc aaataatccc tgataccttt ttgtagaaat attgaccgtg catccgcggt
    28021 caatgttagc tattatgttg cgcccttttt ttacgggtgt taacaaagga ggtatcaacc
    28081 catggccgaa aaaaaacagt ggcatgaaac gctacacgac cagtttgggc agtactttgc
    28141 ggtagataac gttctgtatc atgaaaagac cgatcaccag gatctgatca tttttgagaa
    28201 cgctgcattt ggtcgcgtaa tggcgctgga tggcgtagta caaaccaccg agcgcgacga
    28261 gtttatctat catgagatga tgacccatgt tccgctactg gcccacggtc acgcgaaaca
    28321 tgtgctgatt atcggcggcg gcgacggtgc catgctgcgt gaagtaaccc gacataaaaa
    28381 cgttgagtca atcacgatgg tggaaatcga tgcgggtgtc gtgtcgttct gccgtcagta
    28441 tctacccaac cataacgccg gtagctacga cgatccgcgc tttaagctgg tgatcgacga
    28501 tggcgtcaat ttcgttaatc aaaccagcca gacctttgat gtcattatct ccgactgcac
    28561 cgatcctatc ggtcccggcg aaagcctttt cacttcggca ttttatgaag gctgcaaacg
    28621 ttgcctgaat cctggcggta tcttcgtcgc acaaaacggc gtctgctttt tacagcagga
    28681 agaagccatc gacagccatc gcaaactcaa ccattacttc agcgacgttg gcttttatca
    28741 ggcggcgatc ccgacctatt acggcggtat catgactttt gcatgggcga cagataacga
    28801 cgccttacgc catctctcaa ccgaaattat tcaggcgcgt tttctcgcct ctggcctgaa
    28861 atgccgttat tacaatccgg cagtccatac ggcagctttt gccttacccc agtatctgca
    28921 agacgcactg gcttcacagc cgtcctaagg agaagataag aaattgaaaa aactgaaact
    28981 gcatggcttt aataatctga ccaaaagtct gagtttttgt atttacgata tctgctacgc
    29041 caaaactgcc gaagagcgcg acggttatat tgcttatatc gatgaactct ataatgccaa
    29101 ccgtctgacc gaaatcctgt cagaaacctg ttccattatc ggggctaata ttcttaacat
    29161 cgcccgccag gattacgaac cacagggtgc cagcgtcact attctggtga gtgaagaacc
    29221 ggttgacccg aaactcatcg acaaaacaga acaccccggc ccactgccag aaacggtcgt
    29281 tgcccatctc gataaaagtc atatttgcgt acatacctac ccggaaagtc atcctgaagg
    29341 cggtttatgt accttccgcg ccgatattga agtctctacc tgcggcgtga tttctccgct
    29401 gaaggcgctg aattacctga tccaccagct tgagtccgat atcgtaacca ttgattatcg
    29461 cgtgcgcggt tttacccgcg acattaacgg tatgaagcac tttatcgacc atgagattaa
    29521 ttcgattcag aactttatgt ctgacgatat gaaggcgctg tatgacatgg tggatgtaaa
    29581 cgtctatcag gaaaatatct tccataccaa gatgttgctt aaagagttcg accttaagca
    29641 ctacatgttc cacaccaaac cggaagattt aaccgacagc gagcgccagg aaattaccgc
    29701 tgcgctgtgg aaagaaatgc gcgagattta ttacgggcgc aatatgccag ctgtttaacg
    29761 gctctggcgg agctcccagg ctccgccaga tttatttact tctgctgcac gaaattgcgg
    29821 taagccgcca cgacctgcag aaaatcctca acgccgcata gcgacaggct ttcttcgtcg
    29881 tagtagttca tcccctcttc catttcatcg ccagcgaatt ccagttgatt ggcgcgaacc
    29941 atcacctctt cgccgtccat ccatagcgta tactcatgcc ctgcccgctg ccaggaacgt
    30001 tcgctacctt tcagtgcgtg cgctgcttgt tccacttcat caagcaaggc caggttttct
    30061 ttcacctctt cattaaacca gtgcccgacc acttcatgcc ccatggacat acgcaccttt
    30121 accactccgg taatatcgcg cagaaattcg taatccatag tgtgttcctc tgctcccggc
    30181 aatgcaccga aatcgcattg cgccattgat gtaattatcg cagccttacc ggagaaaaac
    30241 agcggaacgg cgatggttta gaaataaaaa aatgcccggg aatctcattc accgggcatt
    30301 gtgtcgttta tgcgcagcgc gtgcgctgac tttttaaacc gcagtctgga aaatcacccc
    30361 atcggctttc tcggtgtact gagaaagctg gttgaagttc agataacggt aagtatcaac
    30421 ggctgtttta tctacctgcg ccacgtaggt ctggtactct tccggcgtcg gcagtttgcc
    30481 aatcagcgcc gcaacagcag ccagttccgc agaagccagg aagacattcg cgccagtacc
    30541 cagacggttc gggaagttac gggtagaggt ggaaaccacc gttgcaccgt ccgccacacg
    30601 cgcctggtta cccatacaca gggaacagcc agggatctcg atacgcgcac cactcttacc
    30661 gaagacgctg tagtagcctt cttcggtcaa ctgtgcggcg tccatacggg ttggcggtgc
    30721 cacccacagg cgggtcggca actgaccttt gtgcgcatcc agcagtttac ctgcggcacg
    30781 gaagtgaccg atgttagtca tgcaagaacc gataaacact tcgtcgatct tctcgccctg
    30841 taccgcagac agcggacgtg cgtcgtccgg gtcgttcgga gcacagagga ttggctcttt
    30901 aatatccgcc agatcgatgt cgatcactgc cgcgtattcc gcatctgcat cggcttccag
    30961 cagctcagga ttcgccagcc atttttccat gccctgaata cgacgttcca gggtacgacg
    31021 atcgccgtaa ccttccgcga tcatccactt cagcaggacg atgttggagt tcaggtattc
    31081 gatgattggt tctttgttca gcttgatggt acaaccggca gcagaacgct cggcggacgc
    31141 atcggtcagc tcaaaggcct gctcaacttt cagatccggc agaccttcaa tttctaggat
    31201 gcggccagag aagatgtttt tcttgccttt cttctcaacg gtcagcagac cttgtttgat
    31261 cgcatacagc ggaatggcgt gtaccagatc gcgcagggtg atgcccggct gcattttgcc
    31321 tttgaagcgc accagaacgg attccggcat atccagcggc attacgcctg ttgcggctgc
    31381 aaatgccacc agaccagaac ccgccgggaa agagataccg atcgggaaac gggtatggga
    31441 gtcaccaccg gtaccgacgg tatccggcag cagcatacgg ttcagccagg agtggattac
    31501 gccgtcaccc ggacgcagcg acacaccgcc acggttcata atgaagtccg gcagcgtgtg
    31561 gtgcgtgttc acgtcaactg gcttcggata cgccgcggtg tggcagaaag actgcatcac
    31621 caggtcagcc gagaagccca ggcacgccag gtctttcagt tcatcacggg tcatcgggcc
    31681 tgtggtgtcc tgagagccta cagaagtcat tttcggttca cagtacgcgc ccggacgaat
    31741 gcctttcacg ccacaggcac ggcctaccat tttctgcgcc agcgagaagc cgcgatcgct
    31801 ctcagcgaca tctttcgcct gacggaacac atcactgtgc ggcagaccaa gtgcttcacg
    31861 cgctttggtg gtcaggccac gaccgataat cagcggaata cggccgccag cacgcacttc
    31921 atcaatcagc acgtcggttt tcagttcgaa ggtcgccagc agttcgccgg tttcgtggtt
    31981 acgtacttca cctttgtacg ggtaaacgtc aattacgtcg cccatgttca ggttagagac
    32041 atccacttcg attggcagcg cacccgcatc ttccatcgtg ttaaagaaga tgggtgcaat
    32101 tttaccgccg aggcacaaac caccgccgcg tttgttcggc acatgtggaa tatcatcgcc
    32161 cataaaccac agaacggagt tcgtggcgga tttacgcgat gaacccgtac ccacaacgtc
    32221 accgacgtac gccagcggga aacctttctg ttgcagagct tcaatctgtt tgatcgggcc
    32281 aacaacacct ggctggtctg gctcaatacc ttcacgggcg tttttcagca tcgccagcgc
    32341 gtgcaatggg atatccgggc gtgaccacgc atccggtgcc ggagaaaggt catcggtgtt
    32401 ggtttcgcca gtgactttga agacggtaac ggtcagtttt tcagccagcg ccgggcgatt
    32461 caggaaccat tcggcatcag cccaggactg cataacctgc ttcgcatatt cgttgcctgc
    32521 tttcgctttc tcttctacgt catagaagtt atcgaacatc agtagtgtgt gagaaagtgc
    32581 tttggcagcg atcggtgcca gtttggcatc atccagcgca tcgatcagcg gatgaatgtt
    32641 gtaaccaccc tgcatagtac ccagcagttc gatggctttt tccggagtca gcagtgggga
    32701 tttggcttcg cctttggcga cagcagccag gaagcctgct ttaacatagg cggcttcatc
    32761 gacgccagga ggaacacggt tggttaacag atctaacagg aattcttctt cgcccgcggg
    32821 cgggtttttc agcagctcta caagcgcggc catttggttt gcatccaggg gtttgggcgc
    32881 aatcccctca gcggcacgct cagctacgtg cttacggtat tcttctagca cgacggttct
    32941 cctcgctctc attgtcatag tgcggcaggc gattctcttc acgctcctgt gagacagcag
    33001 tttgtagggt aaatgcccag taccgcaacg ggcagaatag caggattttg acgacgtgtt
    33061 aatctgttta caaaaaagca acataaaaaa gtggctgaat cgttaaggat ggtctagaga
    33121 ttgtttccct ccgtaaaaac ttcgttaatt cgcatggtga taatcacctt tctcaacgaa
    33181 tcccaagatt agtcaaaatt taaactaccg cctctttata ctcggattca cagcacctgc
    33241 gggtggcagt tcgccgacca ttgcgatttc cttgagatcc gaattatgaa aatgactttg
    33301 ccgtttaaac cccatgtgct ggcgctaatt tgcagtgccg ggctttgtgc cgcctctgcc
    33361 gggctatata taaaaagccg cacagtggaa gcgcctgtgg aaacgcaatc gacacaactg
    33421 gctgtgtctg acgctgccgc agttacgctt cctgcaacgg tttccgcacc tcccgtaaca
    33481 cccgccgtcg tcaaatccgc attcagcact gcacaaatag atcaatgggt cgcgcccgtc
    33541 gcgctgtatc ccgacgccct actttcgcag gtgctgatgg catcaaccta tccgacaaac
    33601 gttgctcaag cagtgcaatg gtcgcacgat aatccactta aacaaggcga tgctgctatt
    33661 caggcggtat ctgaccagcc gtgggacgcc agcgttaaat cactggtggc ctttccacaa
    33721 ttgatggcat tgatgggcga aaacccgcaa tgggtgcaaa acctgggcga tgcttttctg
    33781 gcccagccgc aggacgtgat ggactcggta caacgattgc ggcaactggc acaacaaacc
    33841 ggctcgctga agtcatcaac cgaacagaaa gttattacca caacgaagaa aactgtaccg
    33901 gtaacacaga cagtcacggc tcccgtcata ccatccaata ccgtttcaac tgccaaccct
    33961 gtcattacag agcctgcaac aaccgtcatt tccattgagc ccggcaatcc tgatgtggtc
    34021 tatattccca actacaaccc aaccgtggtt tacgggaact gggccaatac tgcgtatccg
    34081 ccggtttatc tgccaccacc agccggagaa ccgtttgttg acagctttgt acgcggattc
    34141 ggctatagca tgggcgttgc taccacgtac gcactattca gcagcatcga ctgggatgac
    34201 gacgatcatg accatcatca tcatgacgat gataattatc atcaccacga tggcggtcat
    34261 cgtgacggta atggctggca acataacggc gacaacatca atatcgacgt caacaatttc
    34321 aaccgtatca ccggtgagca tcttactgat aagaatatgg catggcggca caatccaaac
    34381 taccgtaatg gtgtgcccta tcatgatcag gatatggcaa agcggtttca tcaaactgat
    34441 gtcaacggcg gaatgagtgc cacgcagcta cctgctccaa cacgcgacag ccagcgtcag
    34501 gcggcagcaa gtcagtttca gcaacgaaca cacgccgccc ccgtcattac acgagatacc
    34561 caacgtcagg cagcggcaca gcggtttaat gaagctgaac actatgggag ctatgacgac
    34621 ttccgcgact tcagccgtcg ccaaccactg acccagcaac aaaaggacgc cgctcgtcag
    34681 cgttatcagt cagcttctcc tgagcagcgc caggcagttc acgagaaaat gcagactaac
    34741 ccgcagaacc agcagcgaag agaggcagcg cgtgagcgca ttcagcccgc ctcgcctgag
    34801 cagcgccagg cagtccgcga gaaaatgcag actaacccac agatccagca gcgaagagac
    34861 gcagcgcgtg agcgtattca gtcagcctcg cctgagcagc gccaggtgtt taaggaaaaa
    34921 gtacagcagc gcccactgaa ccaacagcaa cgtgataacg cccgccagcg tgttcaatca
    34981 gcatcacctg aacaacgtca ggtttttcgg gagaaagttc aggagagccg cccacaacgt
    35041 ctaaacgaca gtaaccatac tgccaggctg aataacgagc aacggtcagc agtacgcgaa
    35101 cgtctctctg agcgcggagc aaggcgactg gaaaggtaaa ttacaggcgt aaaaaaagcg
    35161 gcgtggttag ccgctttttt aattgccgga tgttccggca aacgaaaaat tacttcttct
    35221 tcgctttcgg gttcggcagg tcagtaatgc taccttcgaa cacttctgcc gccaggccca
    35281 cagactcgtg cagagtcggg tgcgcgtgga tggtcagtgc gatgtcttca gcatcacaac
    35341 ccatttcaat tgccaggccg atttcaccca gcagctcgcc gccgttagta ccgacaatcg
    35401 caccaccgat cacacggtga gattctttgt cgaaaatcag cttggtcata ccgtctgcgc
    35461 agtcggaagc gatagcacga ccagaagcag cccacgggaa ggtggcggtt tcatagctga
    35521 tgcctttctc tttcgcttct ttctcagtca gacctaccca tgcaacttct ggttcggtat
    35581 aggcgatgga cgggataact ttcggatcga agtagtgttt cttaccggcg ataacttcag
    35641 cggcaacgtg accttcgtga acacctttgt gtgccagcat cggctgaccg acgatatcgc
    35701 cgatagcaaa gatgtgcggt acgttggtac gcagctgttt gtcaacgcgg atgaaaccac
    35761 ggtcgtcaac ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc ggcacacgac
    35821 caatcgctac cagcacggcg tcgtaacgct gcggttcagc tggtgctttt ttgccttcca
    35881 tcgtcacata aataccgtct tctttcgctt caacggcggt aactttggtt tccagcatca
    35941 ggttgaattt cttgctgata cgcttggtga agactttaac gatgtctttg tcagctgccg
    36001 ggataacctg gtcgaacatt tcaaccacgt caatctgtga acccagcgcg tggtaaacgg
    36061 tgcccatttc cagaccgatg ataccgccac ccattaccag caggcgttct ggtacttctt
    36121 tcagttccag cgcgtcagta gagtcccaga tacgcggatc ttcatgcgga ataaacggca
    36181 gttgaatcgg gcgagaaccc gctgcaatga tcgcgttgtc gaagttgatc acggttttac
    36241 cgttctcacc ttcaacttcc agggtgttag ccccggtaaa tttacccaga ccgttgacca
    36301 ctttgacttt gcggcctttc gccataccag ccagaccacc ggtcagctga ttgatcactt
    36361 tctctttcca ggtacgaatc ttgtcgatat cggttttcgg ttcgccgaag acgataccgt
    36421 gttcagccag cgctttggct tcttcgataa cttttgctac gtgcagcagt gctttagaag
    36481 ggatacagcc gacgttcagg caaacaccgc caagggtgtt gtaacgttct acgattacgg
    36541 tttccagacc taaatcagcg caacggaagg cagcggagta acctgcgggg cctgccccaa
    36601 gtaccacgac ctgagttttg atttcagtac tcatcatgac ctctatatat ttatctccgg
    36661 cgggtctgac gtttttttta tgacgcccat tatccaccgg gtcgttctat ccgtcggtat
    36721 tttacaaaat tgttaacaat ttttaaacaa caaacggcaa ccgatttgtc tattcgctaa
    36781 taacctcaat acattcatga gattaccaga aaaaagccgg ccgttgggcc ggctctttta
    36841 cttacatcac cagacggcga atgtcagaca gcgtgttgtt aatgatggta atgaaacggg
    36901 caccatcagc accgtcgatc acgcggtggt cgaaggagag agaaatcggc agcatcagac
    36961 gcggcacgaa ctctttacca ttccacaccg gctccatcgc ggacttggaa acgccgagga
    37021 tagccacttc cggcgcgttc acaatcggcg cgaagtgggt agtacccagg ccgccgatgc
    37081 tggagatggt gaagcaaccg ccctgcattt cgcccgcagt cagcttaccg tcacgcgctt
    37141 tcttagaaat agtcatcagc tcgcgagaca gttcgatgat gcctttcttg ttgacgtctt
    37201 tgaataccgg aacaaccaga ccgttcgggg tatccaccgc cacaccgatg ttgatgtatt
    37261 tcttcagggt cagacgctga ccgtcttccg acagcgaact gttgaagcga ggcatctgct
    37321 caagcgctgc agcaacggct ttcatgatga agacaaccgg ggtgatcttc acatccagct
    37381 tacgtttcgc cgcttcttcg ttctgctgtt tacggaacgc ttccagctcg gtgatatcgg
    37441 ttttgtcgaa gtgagtaaca tgcgggatca tcacccagtt acggctcagg ttcgcaccag
    37501 aaattttctg gatacggccc agttccactt cttcgatttc accaaacttg ctgaagtcca
    37561 ccttcggcca cggcagcatg ccagggatac caccgccagt cgctgccgga gctgcttctg
    37621 cacgtttgat agcttctttc acgtaagcct gaacgtcttc gcgcaggata cgacctttac
    37681 ggccagtgcc cttcactttc gcaaggttaa cgccaaactc gcgtgccaga cggcggatca
    37741 gcggggtcgc gtgaacgtaa gcgtcatttt cagcaaattc agatttgcct tccgctttcg
    37801 cagccggtgc tgctgccggg gcttcagctt ttgctgccgg agccggcgct gctgcttcct
    37861 gtttcgcagg agctgccgca ggcgctgcgc cttcaacttc gaagatcata atcagcgagc
    37921 cagttttcac tttatcgcca acgttgactt tcagttcctt cacgacgcct gcaaacggag
    37981 ccggaacttc catagaagct ttatcgcctt ctacggtaat cagtgactgt tcagcggcaa
    38041 ctttgtcgcc cactttcacc atcacttcgg tcacttcaac ttcgtcaccg ccgatatccg
    38101 gaacgttaac ttctttcacg ccagccgctg gtgcaggggc cgctgccgga gccgcttcct
    38161 gtttagccgc cggagctgcc gcgcctgctt cacccgccac ttcgaagacc ataatcagcg
    38221 agccggtaga cactttgtcg ccggtgttca ctttgatttc tttcacggtg cctgcaaacg
    38281 gagccggaac ttccatagaa gctttatcgc cttctacggt gatcagcgac tgctcagctt
    38341 caactttgtc gccaactttc accaggattt cggtcacttc aacttcgtcg ctgccgatat
    38401 ccggaacgtt aacgtctttt gccgcggcag ccgcggggac tgctgccgga gctgcttctt
    38461 tcttctcttc tgcctgagca ggtgcagcgt ctgctgcacc gtcggcggaa tcgaaaatca
    38521 taatcagtgc gccggtctga gttttatcgc caacagagac tttgatctct ttaacgatac
    38581 ccgcctgcgg agacggaact tccatagaag ctttgtcgcc ttctacggtg atcagcgact
    38641 gttcggcttc aactttgtcg cccactttga ccaggatctc ggtgatttca acttcatcag
    38701 ccccgatgtc cggtactttg atttcgatag ccattattct tttacctctt acgccagacg
    38761 cgggttaact ttatctgcat cgatgttgaa tttggcgatt gcgtcagcaa ccactttctt
    38821 atcgatttcg ccacgtttag ccagttcgcc cagcgccgca accacgacat aagaagcatc
    38881 aacttcgaag tggtgacgca ggttctcacg gctgtcggaa cgaccgaagc catcagtacc
    38941 cagtacgcgg tagtcgtcag ccggtacgta agtacggacc tgctcagcga acagtttcat
    39001 atagtcggta gatgccacag ccggagcgtc gttcatcacc tgagcgatat acggaacgcg
    39061 cggagtttcc agcggatgca gcatgttcca gcgttcacaa tcctgaccat cacgcgccag
    39121 ttcggtgaag gaggtcacgc tataaacgtc agaacctacg ccgtaatctt tcgccaggat
    39181 ctctgccgct tcacggacgt gacgcaggat agaaccggag cccagcagct gaactttacc
    39241 tttgctacct tcaatggttt cgagtttgta gataccttta cggatacctt cctcagcacc
    39301 ttccggcatt gccggcatgt ggtagttttc gttcagcgtg gtgatgtagt agtaaacgtt
    39361 ctcttgtttt tcaccgtaca tacgctccag accgtcatgc ataatgacag caacttcgta
    39421 agcgtaagcc gggtcgtaag agatacagtt cggaatagtc agcgactgaa tgtggctgtg
    39481 accatcttcg tgctgcagac cttcaccgtt cagggtggta cgaccggaag taccgccgat
    39541 caggaagcca cgcgcttgct ggtcgccagc cgcccagcac agatcgccga tacgctggaa
    39601 gccgaacatc gagtaataga tgtagaacgg aatcatcggc agattgttgg tgctgtaaga
    39661 ggtcgccgct gccagccagg aacaacctgc gcccagctcg ttgatccctt cctgcagaat
    39721 ctgacctttc tcgtcttctt tatagtaagc aacctgctcg cggtcctgcg gggtgtactg
    39781 ctgaccgttc gggctgtaaa taccaatctg acggaacaga ccttccatac cgaaagtacg
    39841 cgcttcgtcg gcgatgatcg gtaccagacg atctttgatc gacttgttct tcagcatcac
    39901 gttcagggca cgaacgaaag cgatagtggt agagatctct ttgctctgct cttccagcag
    39961 cgcgccgaag tcttgcaggc ttggcagctc aagcttctcg gtgaagttcg gctgacggct
    40021 tggcagataa ccgtgcagtt tctgacgctg tgcgtgcaga taggtatgct cttcagaacc
    40081 ttccgggaag gtgatgtacg gcagtttttc gatatctgca tcagacaccg gcacattgaa
    40141 acggtcgcgg atgtgacgca caccgtccat gttcattttc ttaacctggt gcgcgatgtt
    40201 tttaccttca gccgcgtcgc ccatgccgta acctttaatg gtatgagcaa ggattaccgt
    40261 cgctttgcct ttggtttcct gcgctttctt gaatgcagcg tagattttct tcggatcgtg
    40321 accaccacgg ttcagtgccc agatctgctc gtcagtccag tctgcaacca gtgctgcggt
    40381 ttcaggatat ttaccgaaga agtgttcacg aacgtacgca ccatctttcg atttgaaggt
    40441 ctggtagtcg ccgtcaacgg tttcgttcat cagctggatc agtttaccgc tggtatcttt
    40501 acgcagcagt tcatcccaac ggctacccca catcactttg atcacgttcc agccagcacc
    40561 ttcgaagata ccttccagtt cgttgatgat cttgccgtta ccggtgaccg ggccgtcaag
    40621 acgctgcagg ttacagttga taacgaagac caggttatcc agtttttcac gggtagcgat
    40681 ggtgatcgca cctttggatt ccggttcgtc catttcaccg tcgccgagga acgcgtaaac
    40741 ggtctgttta gaggtatctt tcaggccacg gtgttccaga tatttcagga atttagcctg
    40801 gtaaatagca ccaatcggac ccagacccat agatacggtc gggaactgcc agaattccgg
    40861 catcaatttc gggtgcggat aggaagagag gccattgccg tgaacttcct gacggaagtt
    40921 atccagctgc tcttgagtca gacgaccttc caggaaagca cgagcgtaca cgcccgggga
    40981 gatgtggccc tggaagtaaa ccaggtcgcc gccatcctgc tcgttgcgtg cacggaagaa
    41041 gtggttaaag cacacatcat aaatggttgc ggaagactgg aaggacgcca tgtggccgcc
    41101 cagttcgagg tcttttttcg acgcacgcag aaccgtcatg atggcgttcc agcggatagc
    41161 tgaacgaata cggcgttcca gttccagatt acccggatac tccggttgtt cttcaacggg
    41221 gatggtgttg atgtagttgc tgataccggt gcctgcggct acgttgacac cgcctttgcg
    41281 ggcttcagca agcagttggt cgatcagata ctgagcacgc tcaacacctt cttcacggat
    41341 gaccgattcg atcgcctgga gccagtcgcg agtttcgatc ggatccacgt catttgggaa
    41401 acgttctgac atgggttatt ccttatctat ctaataacgt tgagttttct ggaacctgtc
    41461 ccattgaact ctcgccggaa agctcaataa gacaggttct acgtttagtt gccgcgcttt
    41521 tatatgcgct tgatttacaa catcttctgg ataattttta ccagaaaaat cactaattct
    41581 ttcgttgctc cagacgacgc agagaacgct cacggcggct ttcttcacgg cttctgtcga
    41641 gcaaaatttc ttcgataaag gccagatggc gatgcgatgc ttcgcgcgct tcttccggct
    41701 taccggccat aatcgcttca aatatgcggg tgcggtgact actcaccagc ggcagcatct
    41761 cgcgacgcga atagagcaat tcgaagttct ggcggacatt ctgggccaac atcggctcca
    41821 tacaccttag cagatgaagc agaaccacat tgtgggccgc ttcggtgacg gcaatctgat
    41881 actggagtac ggcgtttgat tccgcgtcca gatcgccaga ctgctgcgcc agttctatgg
    41941 cgtggtggag ttcacggatg cgttccttgt cttcatcggt actacgcagc gcggcgtaat
    42001 aagcggcgat accttccagg gcgtgtcgtg tttcgagcaa gtcatactgt gattcaggat
    42061 ggtcggagag cagctccacc agcggatcgc tgaagctttg ccataggctg ctctggacaa
    42121 aagtgccgcc accctggcga cgaagcaaca agcccttcgc ttcgagacgt tgaatcgcct
    42181 cacgcaatga gggacgggag acgtcaaact gttttgccag ttcgcgttcc ggtgggagtt
    42241 tttcgcccgg gcggagagtg ccttcgagga tcaaaaactc cagttgctgc tcaatcacat
    42301 cggagagttt tggttggcgg attttgctgt aggccatgag ttcctgtctt aagccacttg
    42361 ccgaagtcaa ttggtcttac caatttcatg tctgtgacgc taaagtaaca aagtattcac
    42421 cttatgtcca tacaggtttt gattgaaatc atgaaactgt gcacatttta acaacttgac
    42481 atatataacg tttcaaagtt gtaactatgc acaaatgtag actttacgta ggaaagggat
    42541 tttttaacga ttattaaact ataaaaatcc gaattgaacc gattcactta ccaattttgt
    42601 gatttttaat tcaattaaaa cgaatttaaa ttcattctgc atattgagag gggttgaggc
    42661 tgagctttac aaacggtttc tttttaagca actcatcttc aaccatgcat aaagcgggtg
    42721 cattcgctgc cgcataccat tattcttgat ctgacggaag tctttttgta actattcaaa
    42781 cttctttgat gtaaacaaat taatacaaca aacggaattg caaacttaca cacgcatcac
    42841 tgcgtagatc aaaaaaacaa tcaccgcacg aggtttcatg atggaaggtc aacagcacgg
    42901 cgagcagcta aagcgcggcc ttaaaaaccg ccatattcag cttatcgcgc tgggtggcgc
    42961 gatagggaca gggttattcc tgggtagcgc ctccgtaata cagtccgcag ggccagggat
    43021 tatcctgggt tacgccattg ctggttttat cgcctttctg atcatgcgtc agctgggtga
    43081 aatggtggtc gaagaacctg tcgcaggctc ctttagccac tttgcttata aatactgggg
    43141 cagctttgct ggcttcgctt ctggctggaa ctactgggta ctgtacgttt tagttgccat
    43201 ggcagagctg actgctgtgg gtaaatacat tcagttctgg tatccggaaa tcccaacctg
    43261 ggtttctgcc gccgtgttct ttgtggtgat taacgccatc aacctgacca acgtaacagt
    43321 gtttggtgag atggagttct ggtttgccat tatcaaagtt attgcggtag tagcgatgat
    43381 catcttcggc ggctggctgc tgttcagtgg taacggcggt ccgcaggcaa gcgttagcaa
    43441 cctgtgggat cagggcggtt tcctgccgca cggcttcacc gggctggtga tgatgatggc
    43501 gattatcatg ttctcgttcg gtggtctgga actggtgggg atcaccgcag cagaagctga
    43561 taacccggag caaagtatcc cgaaagcaac taaccaggtt atctaccgca tcctgatttt
    43621 ctatattggt tcgttagccg ttctgctctc actgatgccg tggacccgcg ttaccgccga
    43681 taccagtccg tttgtgctga tcttccacga gttaggcgat acctttgtgg cgaatgcgct
    43741 gaacatcgtg gtactgactg cggcgctctc cgtgtacaac agctgcgtat attgcaacag
    43801 ccgtatgctg tttggtctgg cacaacaggg taacgcgcca aaagcgctgg cgtctgtcga
    43861 taaacgcggc gtaccggtta acaccattct ggtgtctgcg ctggttacag cattgtgcgt
    43921 attgattaac tatcttgctc cggaatccgc atttggcctg ttaatggcac tggtggtatc
    43981 cgcactggtg atcaactggg cgatgatcag tctggcgcat atgaagttcc gtcgcgccaa
    44041 gcaggaacaa ggcgtggtaa ctcgcttccc tgctctgctt tatccgctgg gtaactggat
    44101 ctgcctgctg tttatggcgg tggtactggt gattatgctg atgaccccag gaatggcgat
    44161 ttcggtatac ctgatcccgg tatggctggt ggtgttaggt atcggctatc tgtttaaaga
    44221 gaaaaccgca aaagccgtaa aagcacatta atctctctac gccctcaccc gtacagggtg
    44281 agggcaataa tctttacacc aacgccccgt aaatcgtcag tagcgcaatc accaccacga
    44341 ccacgaacga ggttttcttc gccattgaaa ccgctgcctt cggcgtctcc accttatcga
    44401 catgcggttc acgcgccaga gagaactgcg ccagacgcgt taatacctga tattgcgaag
    44461 tacggaaatc acccagcgaa gcaaaccagg ccggtaacgc tttctcacca tgaccgatca
    44521 aggcatatac cacacctgca agacgaaccg gcacccaatc cagtacatga agcactgcat
    44581 caatgccgga ctgtaaacga tgatgcggcg tctggtatcg cgccagccag tattgccatg
    44641 cacgcaaaaa cgcatacccc atcagcgtaa cgggtcccca ggttcccccc acaatcagcc
    44701 agaacagcgg tgcaagataa aaacgaaagt taatccacag caatgcattt tgcagctcac
    44761 gcaaatactc acgttcgtcg cagcctgccg ggacgccgtg aatcatggtg agttcgccag
    44821 ccatcgtggc acgggcatgg ctatcattac gtgaagcagc tgtcagataa gcatgataat
    44881 gaagacgaac tttacctgcg ccaatacaca gcaaaccaat cagcagccac accagcagcg
    44941 tgggaacgtt gaacaatact ccctgcaatg cgcgtaacag taaaaaagtc acgcccatcg
    45001 caataatggt catgcctaac gtgcgcccga gagaaaaatg tttcacccgc cgaaagaacg
    45061 cttcaagacg atgatcaagc tgccagtgct cgcccaactt aaacaggcgc tcgaaaatta
    45121 acaccagtaa ggttgtaaat agcgtcatgt tgtctccttg ctgaccagcg cacgaaaccg
    45181 tgcccaatca aatgcaggac cgggatcggt tttccgatcc ggcgcaatat cacaatggcc
    45241 cgtcatgtta ttagcgatat ccggatagca atcaatcagt gcccgcgtaa ccgccgcaag
    45301 ctgttgatac tgcgcatcgg tatacgccag cgtatcggtg ccttcaagct caatcccaat
    45361 agaaaaatca ttgcagcgtt cgcgcccctg atactgagag actcccgcat gccatgcacg
    45421 tttatcgaaa ggaacatact ggactatttc accatcacgg cgaatcaaac agtgagcgga
    45481 gacgcgcaaa tgggcgatct cagcaaagaa aggatgtgcc tgcggatcaa tagttccagt
    45541 gaataatgcg tcgatccacg gaccgccaaa ctcgcctggc ggcaggctaa tattgtgcac
    45601 caccagcagg gtgggtgttt cgtcatccgg gcggcaatcg taatgtggtg agggaacgcg
    45661 gcgcgcgcca accagccacc cctgttctaa caacatgcag gatctcctta tatgtggtgc
    45721 taatacccgg ttcagagtag catgtttcta ccttatgatt cgttagctat ctggagtttt
    45781 aacatgccgc ctcgccgcta taaccctgac acccgacgtg acgagctgct ggaacgcatt
    45841 aatctcgata tccccggcgc ggtggcccag gcgctgcggg aagatttagg cggaacagtc
    45901 gatgccaaca atgacattac ggcaaaactt ttaccggaaa attctcgctc tcatgccacg
    45961 gtgatcaccc gcgagaatgg cgtattttgc ggcaaacgct gggttgaaga ggtgtttatt
    46021 caactggcag gcgacgatgt caccataatc tggcatgtgg atgacggcga tgtcatcaac
    46081 gccaatcaac ccttgttcga acttgaaggc ccatcccgcg tgctgttaac gggcgaacgc
    46141 actgcgctta attttgtgca aaccctttca ggagttgcca gtaaggtacg ccactatgtc
    46201 gaattactgg aaggcaccaa cacgcagttg ttggatacgc gtaaaacctt acccggtctg
    46261 cgttcagcgc tgaaatacgc ggtactttgc ggcggcggag cgaatcaccg tctggggctt
    46321 tctgatgcct tcctgatcaa agaaaaccat attattgcct ccggctcagt gcgccaggcg
    46381 gtcgaaaaag cgtcctggct gcacccggat gcgccagtag aagtcgaagt agagaatctg
    46441 gaagaacttg atgaagccct gaaagcagga gccgatatca tcatgctgga taacttcgaa
    46501 acagaacaga tgcgcgaagc cgtcaaacgc accaacggca aggcgctact ggaagtgtct
    46561 ggcaacgtca ctgacaaaac actgcgcgaa tttgccgaaa cgggcgtgga ctttatctcc
    46621 gtcggtgcgc taactaagca cgtacaggca ctcgaccttt caatgcgttt tcgctagttg
    46681 ctgacagatt tatccgctcc atagtgggcg gataatctcc ccttccccaa ccttcttcgt
    46741 aacgcctcgc aaatttcttg tctttcgctg caacgaaata aaaaacctcg gaagcagctt
    46801 tcccgtttcg ttttttgccc ctcgcctgtg ctgatggcgt ttgccaaagt agcaccaacc
    46861 aaatcaagga gcgaaacaga tggacaagca acgcggtttt acacttatcg aactgatggt
    46921 ggttattggc atcattgcca ttttaagcgc cattggtatt cccgcttatc aaaactacct
    46981 gcgcaaagcc gcactcaccg acatgctaca aacctttgtg ccttaccgta ccgccgtaga
    47041 gttgtgcgcg ctggaacatg gtggattaga tacctgcgac ggtggcagca atggcattcc
    47101 ctcgcctacc accacccgct atgtttcagc catgagtgtg gcaaagggcg tggtgtcgct
    47161 gaccgggcaa gaaagtctca atgggctaag cgtcgtcatg acgccaggtt gggataacgc
    47221 aaacggcgtc accggctggg cgcgcaactg caatattcaa agtgacagcg cattgcagca
    47281 agcctgcgaa gatgtcttcc gctttgatga cgccaactaa ggagcggcaa tgaatattcc
    47341 acagctcacg gccctgtgcc tgcgttatca gggagtcttg ctggatgcca gcgaagaagt
    47401 ggttcatgtt gcggtggtcg atgccccctc acatgagttg ctggacgcat tgcatttcgc
    47461 taccaccaaa cgtattgaga tcacctgctg gacgcgccaa caaatggaag gtcacgccag
    47521 tcgcacacaa cagacattgc ccgtagctgt tcaggagaag catcagccca aagcagagtt
    47581 gctgactcga acgttacaat ctgcgctgga acaacgcgcg tctgatattc atatcgaacc
    47641 agcggacaat gcctaccgca tccgcttgcg tatcgacggc gtattgcatc ctttaccgga
    47701 tgtttcaccg gatgccggag tcgcattaac cgccagatta aaagtgctgg gaaacctgga
    47761 tattgcggaa catcgcctgc cgcaggacgg gcaattcact gtcgaactgg caggaaacgc
    47821 cgtctcattt cgtattgcga ccttaccatg tcggggtggt gaaaaggtgg tattaaggtt
    47881 gttacagcag gtgagccagg cactggatgt taacacgctg ggaatgcagc cgttacaact
    47941 ggcgggcttt gctcatgcct tgcaacaacc acagggactg gtgctggtaa ctggccctac
    48001 cggcagcggc aaaacggtca cgctttatag tgccctgcaa aagctgaata ccgctgacat
    48061 taatatttgt agcgtcgaag atccggttga gatccccata gccggactaa accagacgca
    48121 aatccatccg cgtgccggac tcacctttca gggcgttttg cgtgcgttat tgcgccagga
    48181 tcctgacgtc atcatgatcg gagagatccg cgatggcgaa acggcagaaa ttgccattaa
    48241 agccgcgcaa accggtcacc tggtgttgtc taccctacac actaattcca cctgcgaaac
    48301 gctggtacgt ttacagcaaa tgggggtcgc ccgctggatg ctatcatcgg cgcttacgct
    48361 ggtaatagcc cagcgtctgg tacgcaaact ttgcccacat tgtcgccggc agcaagggga
    48421 gcccatccac attccagtca atgtatggcc gtcgccgctg ccccactggc aagcacccgg
    48481 ttgtgtacat tgctaccacg gtttttatgg tcgcactgcc ttatttgaag ttctgcccat
    48541 aacaccggtc atacgtcagc ttatttccgc taataccgac gttgaatcgc tggaaacgca
    48601 cgcccgacag gcgggtatgc gaacgctttt tgaaaacggc tgcctggccg tggagcaagg
    48661 cttaaccacc tttgaagagt taatccgcgt attggggatg ccgcatggcg agtaagcaac
    48721 tctggcgctg gcatggcata accggcgacg gcaatgcgca agatgggatg ctatgggcag
    48781 agagccgtac tttactgctt atggcactac agcaacagat ggttacccca ctaagcctga
    48841 agcgaattgc tatcaattct gcgcagtggc gaggagataa aagcgcggaa gtcattcatc
    48901 aactggcgac gctacttaaa gccgggttaa cgctttctga agggctggca ctgctggcgg
    48961 aacagcatcc cagtaagcaa tggcaagcgt tgctgcaatc tctggcacac gatctcgaac
    49021 agggcattgc cttttccaat gccttattac cctggtcaga ggtatttccg ccgctctatc
    49081 aggcgatgat ccgcacgggt gaactgaccg gtaagctgga tgaatgctgc tttgaactgg
    49141 cgcgtcagca aagagcccag cgtcagttga ccgacaaagt aaaatcagcg ttacgttatc
    49201 ccatcatcat tttagcgatg gcaatcatgg tggttgtggc aatgctgcat tttgttctgc
    49261 cggagtttgc cgctatctat aagaccttca acaccccact accggcacta acgcagggga
    49321 tcatgacgct ggcagacttt agtggcgaat ggggctggct gctggtgttg ttcggcatta
    49381 tgctgacgat agccaataag ttgctgatgc gccgcccgac ctggcttatc gcgcggcaga
    49441 aattgctgtt acgcatcccg attatggggt cactgatgcg gggacaaaaa ctcacgcaga
    49501 tctttacgat tctggcgctg actcaaagtg caggcattac ttttttacag ggcgtagaga
    49561 gcgtcagaga aacaatgcgc tgcccgtact gggtgcaact tctgacacaa atccagcacg
    49621 atatcagtaa cggtcatccc atctggctgg cgttaaaaaa tgccggtgag tttagcccgc
    49681 tctgtttgca attagtgaga acgggagagg cctccggctc gctggatctc atgttagaca
    49741 acatcgccca tcatcatcgg gataacacaa tggcgctggc ggataaccta gcagccttac
    49801 tggaaccggc gttgctgatc ataacgggag gaattatcgg tacgctggtg gtggcgatgt
    49861 atctgccaat tttccattta ggcgatgcga tgagtgggat gggataacgc tggcgtgttg
    49921 ctccacacca gcgttggaag attacaggtt gttgaagatg cggttttctt gttcctgcac
    49981 acggataaat gtggtgcgct tggtcagttc tttcaggcgt gaagccccaa cgtatgtaca
    50041 agctgaacgc aggccgccca aaatatcacg cgcggtattt tcaaccgggc ctcgcagcgg
    50101 cagcttaacg gttttacctt ctgctgcgcg atattcagca acgccgccaa cgtgacgttt
    50161 catcgcagac tcggagctca tgccgtagaa cagcataaat ttctcgccgt tctcctcaac
    50221 gatgcgaccg ccgctctctt cgtggcccgc cagcatgccg ccaagcatga cgaaatcggc
    50281 accgccacca aaggctttcg ccacatcgcc cggcgtggtg cagccaccat cgctgatgat
    50341 cattccgccc agaccgtgcg cagcatcggc acattcgatt accgcagaaa gttgcggata
    50401 accgacgcct gttttgacgc gagttgtaca aacagaacct gggccaatgc caactttaac
    50461 gatatcggca cctgagagga taagctcctc acacatttca ccagtcacta cgttaccagc
    50521 acaaatggtt ttggtcggcc acgcttcacg cgctttcgca acgaactgca cgaagtgttc
    50581 ggaataacca ttcgccacgt caatacaaac gaagtttaat gccgggttca ggtcgagaat
    50641 ctgtttagtt ttttcgaaat ccgcatcaga cgtaccggta gaaaccatca catgtttcag
    50701 cacatcagcg gaagaattgt tgataaacgc ttgccactct tcgacagaat agtgtttatg
    50761 cacagcagtc aaaatatcaa aagaagccag cgcagaggcc atagaaaatg tgcctacggt
    50821 gtccatattt gcggcgataa tcggcacgcc ggaccagctc tgacctgaat gtttgaaggt
    50881 gaattgacgt tccagttcaa catcggaacg gcttttaaga gtggagcgtt tagggcggat
    50941 gagaacgtct ttaaaaccta acttcagatc ttcttcaata cgcatgtgcg gattcctggg
    51001 gttaatggct ataagggtaa gagcgcaact ccagtgacgc tatcatacgc actaatcaat
    51061 gcggcgcaag actgcgaaat tgtctttttt tacgctataa tcccataaat ttacctggta
    51121 aatagtgagc gaacgtttca tcaatttcgc tttcgtgcgc tcatttttaa cgttttgata
    51181 atcatgatga attcccggga atataaatta tgaggtatat agttgcctta acgggaggca
    51241 ttggcagtgg caagagtacc gttgccaatg cgtttgctga tctcggaatt aacgtcattg
    51301 atgccgatat tattgcgcgt caggtggttg aaccaggtgc acctgcgcta catgccattg
    51361 ctgatcactt tggcgctaat atgattgctg ctgatggaac attgcagcgc cgggccttgc
    51421 gcgagcggat cttcgccaac ccggaagaga aaaactggct taacgccctg ctgcatccgc
    51481 tgattcagca agagacgcaa caccagatcc agcaagctac ttccccctat gtactgtggg
    51541 ttgtgccatt gctggtagaa aactcactgt ataaaaaagc gaatcgagtg ctggtggtgg
    51601 atgtcagccc agaaacgcaa cttaagcgca ccatgcagcg cgatgatgta actcgcgagc
    51661 atgtcgaaca aatccttgct gctcaggcaa cgcgcgaagc ccgccttgcc gtggcagatg
    51721 acgtcattga taataacggc gcaccggatg ctatcgcatc ggatgttgcc cgcctgcacg
    51781 cacactattt gcagcttgcg tcgcagtttg tctcacagga aaaaccgtaa tgcagaccca
    51841 ggtccttttt gaacatccac taaatgaaaa aatgcgtaca tggctgcgca ttgagttttt
    51901 gattcagcaa ctcaccgtta atttacccat cgttgaccac gctggcgcgc tgcatttctt
    51961 ccgtaatgtt agtgaattac tggatgtttt cgagcgcggc gaagtccgca ctgagctgtt
    52021 gaaagaactt gaccggcagc aacgtaaact ccagacctgg attggcgtgc ctggcgtgga
    52081 ccagagccgt attgaagcat taattcagca gttaaaagcg gcggggagcg tattaatttc
    52141 cgcgccgcgt atcgggcaat ttctgcgtga agatcgtttg attgctctgg tgcgtcagcg
    52201 actgagcatc ccaggcggct gttgcagctt tgatttacct acattgcaca tttggctgca
    52261 tctaccccag gcgcagcgcg actgccaggt agaaacctgg attgccagcc tgaacccgct
    52321 cacccaggca cttaccatgg tgctggattt aattcgccag tcggccccct tccgtaaaca
    52381 aaccagcctg aatggttttt atcaggataa cggtggcgat gccgacttgc tgcgcctgaa
    52441 tctgtcgctc gattcacagc tttatccgca aatttccgga cataagagcc gttttgccat
    52501 tcgttttatg ccgctggaca ctgaaaacgg acaggtaccg gaacgtctgg atttcgaact
    52561 ggcctgttgc taaggagtaa aaatgtcaga aactattacg gtgaattgcc caacctgcgg
    52621 gaaaacggtg gtgtggggtg aaatcagccc gtttcggcca ttttgctcca aacgttgtca
    52681 gctgatcgac ctcggagaat gggctgctga agaaaaacga atccccagca gtggcgatct
    52741 ttccgaaagc gatgactgga gcgaagagcc aaagcagtga catttgtctg atgccgcacg
    52801 taggcctgat aagacgcgga cagcgtcgca tcaggcatct tgtgcaaatg tcggatgcgg
    52861 cgtgaacgcc ttatccgaca tacacacgct taagcttcgc aattaccggt tcattggctg
    52921 gcggaaaatc atcggcatta agaccgacta gcgacatcca cttaccgggt tgcccttctt
    52981 taccccacgg ctccccttcc cagctttcga ccagccagaa ccacagagtt atatgcctgt
    53041 ccgggaattc atattccagt ttttcaaata gcgaaaaatg ttgtggggta atcccgactt
    53101 cttcctgaag ttcacgcacc accgcctgtt ccggcgtttc acccatttca attttaccgc
    53161 cgggaaactc cagtttattc gccatgtgcg catctgctgc gcgacgcgtt ataaagattt
    53221 cattgttctc gttgcgaata atacctaccg caatttgcag ctttttcatt gtcttaaacc
    53281 tataaaaaag gcgcagaatc ctgcgccttt tacttcaaca gttagctttt attgcaggcg
    53341 gccatggcac tgcttgtatt ttttaccaga accgcacggg caaggatcgt tacgtcctac
    53401 tttgcgctca ccggtttgcg ccgccagtgc agctgcggct gcagagtcgt catcctgatg
    53461 gctaagctgc tgcatttgcg ctaaacgctc ggcttccata cgacgctgtt gttccagctc
    53521 ctcaacctct tcaggcatac gtacctgaac tttgctcagc gtactgataa cttcatattt
    53581 caacgactcc agcatcgctg caaacatgga gaacgattca cgtttgtatt cctgcttcgg
    53641 atctttctgt gcatagccac gcaggtggat accctgacgc agatagtcca tcgctgccag
    53701 gtgctctttc cacagggagt caagcgtttg cagcatgacg cctttctcga agtgacgcat
    53761 catctcagca ccaaccactt cttctttacg ctgatacact tcgatggact gcgccagaat
    53821 gcgctcacgc agcgtctctt catgcagttc tggttcttta tccagccact cagcaattgg
    53881 taaatcgagg tcgaaatcgt tcttcagacg ttcctgcagc cccggaatat cccacatttc
    53941 ttccagcgac tgtggcggaa tgtaggcatc aatggtcgct ttgaacacat cttcacgaat
    54001 gctgttaatg gtttcgctca catcgctgac atccagcaat tcgttacgct gggagtaaat
    54061 ggcgcgacgc tgatcgttag ccacgtcatc atattccagc agttgcttac gaatgtcgaa
    54121 gttacgactt tcaactttac gctgggcgtt ggcaatcgct ttagtcaccc acgggtgttc
    54181 aatggcttcg cctggcttca tacccagttt acgcatcatg ccggatactc ggtcggaagc
    54241 aaaaatacgc atcagcgcat cttccatcga caggtagaaa cgggaagaac cagcatcccc
    54301 ctgacgacca gaacgaccgc gcaactggtt atcgatacga cgggattcgt gacgctcggt
    54361 accgatgata tgcaggccac ctgctgccag taccgcatcg tgacgtacct gccagtcggc
    54421 tttaattttt tcaatttgct ctgcggtcgg attttccagc gcggcaactt ctgcctgcca
    54481 gctaccaccg agcacaatat ctgtaccacg acccgccatg ttggtcgcga tagtcaccgc
    54541 agccggataa cctgcctgag caacaatcgc cgcttcgttg gcgtggaatt tggcgttcag
    54601 gacattgtgc ttaataccgg ctttggtcag ttcgtttgac accagctccg atttttcgat
    54661 ggagatagta cccaccagca ccggctggcc tttcgcagta cgttctttga tatcttcaat
    54721 gatcgcctga attttttccg cttcagtcat gtagaccagg tccggcagat ctttacgaat
    54781 cattggacgg ttggtcggaa caacgacggt atccagtttg tagatggagc taaattcgaa
    54841 agcttcggta tcagcagtac cggtcatccc cgccagtttt tcatacagac ggaagtagtt
    54901 ctggaaggtg atcgaagcca gcgtttggtt ttcgttctgg atctgcacac cttctttcgc
    54961 ttccacagcc tggtgcagac catcggacca gcgacggccc tgcatggtac gaccggtgtg
    55021 ttcgtcaacg atgataactt caccatcttt aacgatgtag tcgacgtcac gggtaaacag
    55081 cgcatgagcg cgcagcgccg ccgttacgtg gtgcatcagc atgatgttgg ccggagagta
    55141 cagagactcc ccttcatcca tgatgccctc tttcaccagc agttcttcaa tcagcaccag
    55201 accacgttcg gtcaggttca cctggcgaga tttttcgtcc accgagaagt ggccttcgcc
    55261 ctggaaggtt tcggagtctt ctttttcctg acggatcagg tgcggaataa ttttattcac
    55321 gcgtttatac atttccgagc tgtcttctgc cgggccggaa atgatcagcg gtgtacgcgc
    55381 ttcatcgatc aggatggagt ccacttcgtc caccagcgca tagtgcagtt tacgctgtac
    55441 acgttcttca gggctgaacg ccatattgtc gcgcaggtag tcaaagccgt attcgttgtt
    55501 cgtaccgtaa gtgatgtcag ctgcgtaagc ttcgcgcttt gccggtgctg gcatgcccgg
    55561 caggttgata ccgacagtca ggccaaggaa ttcaaacagc ggacggttgt tttcggcgtc
    55621 acgttgcgcc aggtagtcgt tgacggtaac tacatgcacg cctttaccgg ttagtgcgtt
    55681 cagataagca ggcagcgttg cggtcagggt tttaccttca ccggtacgca tttcggcgat
    55741 gcagcgttcg ttaagaacca taccgccgag taactgaacg tcgaagtgac gcataccaaa
    55801 gacgcgctta cttgcctcac gtaccacggc gaaagcttcc gggatcagat tttccagcac
    55861 ttcgcctttt tccagacgtg cacgaaactc tgcggttttc cctttcagtt cttcgtcgga
    55921 gagtttttcc atctccggtt ccatggcatt gatgatgttg accactttgc gcatccggcg
    55981 cagggtgcga tcgttacgac taccgaaaac tttagttaat aatttgatta gcataataaa
    56041 atctcaaacg ccccgcgttg cggagttaat aaaatgaagt aaaggtttat tgttgttagc
    56101 tgaggcgttg agggccagca cggatgcctt gcgcctggct tatccagacg ggcgtgctga
    56161 attttgcttg tggggtaaaa tgcgcataat caatgcgata acccttttca gacggcgtgc
    56221 cttcctgggt cagcagcgcg ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag
    56281 gcaaagattc ttcagcaacg ggcagcgttt gcggtgccat tgcgaaggaa agatgacgga
    56341 ttaccgtgcg aatggcatgt tgatgccagt aatctacgga atagttcgaa ttcgggcggc
    56401 gcgtgttcgc ttccagcaag gccaattgac caaagttaac tttggctgaa ggctcgtggt
    56461 tgcgggttgt cgcttttgcg ggcgcgtttg gttcggcggc gttgctgagc gcaggcaaac
    56521 ctaaactcgc cgcaaccatc cctaataaga gatgcggcca gaagtagcgt ttaccaaact
    56581 gtcgccagcg cgtcagtatt ccactcacgt tattgccatc ccgatcgtat tcaaacgtat
    56641 ttagcgcaca aatcttatca ttaaagcctg aagtccacag cacgaatgaa gataagttgt
    56701 ccgtaaaagt gcttaagaaa acagcgttcg caccgttttt tatagctctt ataaaattca
    56761 aaaaaaaacg actggttcac ctggccggag agagtgccag atttgccagt cgaattttat
    56821 acgacagtat aaatgtcgtt atgccagtac ggctgaaggc gctttgaagg ccaacggcag
    56881 ttctgcgtcg tcctggaagg tcacatattc ccaggcttcc tgtttcgcca ggacagcctg
    56941 cagcagtttg ttattcagtg catgaccgga tttataagcg gtaaatgcac caataatatt
    57001 gtgaccacac atgaacaagt caccgatcgc atcgagcatt ttgtgacgca caaattcgtc
    57061 ttcaaaacgc aggccgtctt cgttcagtac gcgataatcg tcaacaacga tggcacaatc
    57121 gaagctgccg cccaggcaca aaccacggga ctgcagatat tcgatatcac gcatgaaacc
    57181 gaacgtacgc gcacggctga tctggcgcat aaacgcatca gcggagaagt tcatcgcata
    57241 gcgctggttg ctggaatcaa tagccggatg gttaaaatcg atggtgaaat ccagcgaaaa
    57301 accattgtac ggcttaaatt cagcccactt atcgccatct tcgacacgaa cagtctcttt
    57361 gatgcgaaca aattttttgg cgcagttcaa ctcgtcgata ccggcgtcaa gcagcaggta
    57421 tacaaacgga gcggcgctgc cgtccatgat cgggatttcc ggcgcgttaa cttcgataac
    57481 aatgttatcg atgcccaagc ccgcgagagc agcattgagg tgctctacgg ttgaaatccg
    57541 tacatcatgc tcgttgacca gacacgtaca gagcatggta tcacgcacag atttggcatc
    57601 ggccgggaaa tctaccggtg gattcaagtc ggtgcgacga tagatgaccc cggtgttggc
    57661 cggcgcaggg cgtaacgtca gggtgacttt cttgccggta tgtaaaccga cacccgtcgc
    57721 ctgaacgata cgtttaagtg tcctttgttt gatcatcgta ttatctcgcc aaattaccta
    57781 tccaaccgaa gtgtactata cattcggcgg gccagtttag cacaaagagc ctcgaaaccc
    57841 aaattccagt caattcttaa tcagcttgct tacgcaggaa tgctgggata tccagataat
    57901 ccggctcttt cgcagtttgc ggcgcattgt cattcacgac tttagcaacc ggcttctgct
    57961 cctgggtcag cggagccatc ccatgctgct ggtagcgatc catcactggc tgctgaacct
    58021 gcttattggt caccagagtg atttcaggac gtttgtccat gccgatacct gtcgcaacaa
    58081 cggttacgcg caactcgtca ttcatatccg ggtcaagaga agtaccgata accacagtcg
    58141 cgttgtcgga agcaaatgca cggatggtgt tacctaccgt ttcgaactca tccagacgca
    58201 ggtcgaagcc cgctgtgatg ttaaccagca cgccgcgtgc gccagacagg tcgatatctt
    58261 ccagcagcgg agaagagata gccatttcag cagcttcttc cgcacggtct tcaccgctcg
    58321 ccacgccaga acccatcatt gcgtagccca tttcagacat tacggtgcgt acgtctgcaa
    58381 agtccacgtt catcaaaccc ggacgagtaa tcagttcggc gatgccttgc acagcgcctt
    58441 tgagtacatc gttcgctgcg ccaaacgcat ccagcaggga gataccgcgg cccagaactt
    58501 tcagcagttt gtcgttcggg atagtgatca gagagtccac atgcttggac agttcagtga
    58561 tcccctgctc cgcgaatgcc atacgcttct tgccttcaaa gttgaagggc ttagtgacga
    58621 cagcaacggt caggataccc aaatcttttg ccacttcagc gacgactggt gctgcacctg
    58681 taccggtacc accacccata cccgcagcaa taaagaccat gtctgcacct tccagcgccg
    58741 cacgcagtgc atcgcgatcc tcatctgccg cattgcggcc aacttctgga ttagcgccag
    58801 cgcccagtcc tttggtgata ccgctaccga tttgaatcgt ctgtccaacc gctgttttac
    58861 gcagcgcttg tgcatcggta tttaccgcga agaattcaac accttcaatg cgctcgcgca
    58921 ccatgtgttc aacagcatta ccgccgccgc cgccgacgcc gatgacttta atcaccgcgt
    58981 cattggtaag ttccattggt tcaaacatag tttctctccg atttgtgcct gtcgcctgag
    59041 gccgtaatca tcgtcggcct cataaaaatt aaaactcttt tcgcagccaa ctattgagtc
    59101 gcttgatcca cgagccaact gatgctgtaa cacgtttttc tacttcagct tcaccgttaa
    59161 gatgtgactc tttcccatag tgaagcaatc ccaccgccgt cgaataatac ggctcctgag
    59221 cataatccgt taaaccggta atgttcagcg gcgcgccgat acgcacttgc gtatgaaaca
    59281 cgcgctgagc acaggctgca agaccttcga tctgcgctgc gccaccggtt aatacaatgc
    59341 ctgccgccag gtgatgttta accccttgtt ggcgaagctt ttcctgcaac tgcaatatct
    59401 cttcgttgac caggttgagc agctcggtat agcgcggctc gatcacctct gccagggtct
    59461 gacgttgcag actccgcggc ggacgaccgc ctacgctcgg cacttccacg ctctcatctt
    59521 ttccaacgat ggaacccagc gcacaaccgt ggcgaacttt aatcgcttcg gcgtcgcttg
    59581 gcggcgtgcc aaaggcgtaa gcgatatcac tggtcacgac attgccagca taaggaatta
    59641 ccttagtgtg gcgcaatgcc ccaccggtat aaacggcgat atccattgta ccaccaccga
    59701 tatcgacgac gcagacaccc agttcacgtt catcttccgt caataccgaa taacttgatg
    59761 ccagaccggc aaatatcagt tggtcaactt tcagcccaca acgttcaacc gctttgacga
    59821 tgtttttcgc catatcgttg tggcatgtga tcaggtgcac ttttgcctgc atccgcacgc
    59881 ccgaaagccc taccggattc ttgatccctt cctgatagtc aatcgcatac tcttgcggga
    59941 tcacatgcag cacacgatgc tcatcgcgta cacgcaccga tttcgcggta tggacgacgt
    60001 tttccacatc ttcttgcgtt acttcttctt cagaaatagg caccatacca atttcattct
    60061 ggcagctgat gtgcttacca gaaagcgcca gatataccga agagatctga caatctgcca
    60121 tcaattctgc ctggtcaatg gcacgttgta cgcacttgac cacggattcg aggtcgttca
    60181 ccccgccttt atccatacca cgcgacgggc agctgcccac gccaatgata ttgaccatac
    60241 cgtcgggcag aacttcccct actaaagcgg caaccttcgc ggtaccaatc tccagtccta
    60301 ctaccagttt tctgtccgtc gccttgatca ttgttgttct gcctgtgcct gattttgttg
    60361 ctgagtagat tcctctggcg gcaagggcgc ccagcctact gccgctccag agtcataacg
    60421 caaatcaacg tagctaatcc gtttgccatc ggtttgcgcc tgctgctgta gaaccggata
    60481 aagttctaca aagcgagcca aacgtttcat cgtatcgccc cggccaagat tgagcttaat
    60541 atcgttattc agcgtcaact gccaggaacg ccgcgcggtc atcgccgctt ccttcagagt
    60601 aaatctgtcc tttgccagca tctgccccat ttcgcgatag ccctgcaaca cttcattggc
    60661 gctgccttcc gggccataca gcattggaag cacctgcttg ctggtgcgat ctggcggcac
    60721 gctgaaggta tttccttccg cgtctaccat gtgttgatca ttccaccgcg caatcggcac
    60781 atattcaacc agatgaatct tcaattcatc aggccactgc tttctgacgc tcacctgctt
    60841 aatccacggc agacgttgtt ctatttgcgt ctggatgatg ttgacatcct gggtcataaa
    60901 ggtacccggc tcacccaatg ccaggatcga ctgccggata tcgtcattac gcgtgtaatg
    60961 gcgttcaccg gtcaacacca gctttgagag cggcaggcgt tgcgcatctt ccatccagcc
    61021 caacacgacc cagccgctca ccaacactgt cgttaaaacg gtcagcagga aaaggatccc
    61081 cgccagacgc gttccattat tgcggcgaga agaaacctct tcttcgctgt ttcgcgtgtt
    61141 cagagcagcc tgcgacatat tagtccgcca gttccagaat tcgtactacc aactgcgaga
    61201 agctcatacc tgcctgacgt gccgccatcg gcaccaggct gtggctggtc atacccggtg
    61261 aggtattggc ttccagcaga taaaactgtc catcgctgtc cagcataacg tcaatacgtc
    61321 cccatccttt gcaacctaac gtcgtccatg ctttcagcac taatgcctgc aaattggcct
    61381 cttgtgacgc ttccagacct gcggggcaga aatactgtgt ctcatcagag agatacttcg
    61441 cctcataatc atagaaggtt ccggacggtt gaatacgtac tgacggtaaa atttcttcac
    61501 cgagtatcgc aaccgtgaac tccggcccac ttagccattt ttcaatcaat acttcttcat
    61561 cgtgctgaaa tgccaatctt aatgcatctt gtagagcatt ttctgctact acttttgaca
    61621 ttcccacact ggaaccttcg cggctcggct taacgataac cggcaaaccc agagcagaaa
    61681 tttctgctaa ctgcttatca ctcaggcctt tttcaaactc tacgcgggtt aacgctaccc
    61741 acggcgcgac cggtaaaccg gcaccttgcc acagaagttt gctgcgtagt ttatccattg
    61801 aaagcgcaga tgccatcact ccgctgccgg tataaggcaa gcccatcagc tcgagcatcc
    61861 cctgcagcgt accatcttca ccgccgcggc cgtgtagcgc gataaacact ttctgaaagc
    61921 ccatcgactt cagttgcgtc acgtcaactt ctttcgggtc gacaggatat gcgtcaatac
    61981 cgccttcacg cagcccggct aacaccgctg cgccagaatt cagagaaact tcccgctcag
    62041 cggaagtccc gcccaacagg accgcgattt tatcagtcat gttgttcttc ctccggagtt
    62101 tgcggcttca gtttgatttc agctaaagaa cgggcaattt ttccgatatt accagccccc
    62161 tgaacgagaa tcaggtcgtt accggttaat accggtgcca gcatctcggc tacctgcgca
    62221 gggtccggca ccagaatggg atctattttc ccacgtccac gaattgtgcg acacagcgaa
    62281 cggctgtccg cacccggaat tggcgcttcg ccagccggat acacttccag catcaacagg
    62341 gtatcaacct gcgtcagcac attggcgaaa tcatcataca ggtcgcgtgt acgggtaaaa
    62401 cggtgcggct gaaacagcat taccaggttt ttatccggcc agcctgcgcg cgccgcttta
    62461 atggtggcgt ccacttccgt cgggtggtgg ccgtagtcat cgaccagcat tgccgtaccg
    62521 cttttaccat tcactggctc cagcgggaat tcaccgagga aatcaaaacg gcgaccagtc
    62581 ccctggaagc tctccagcgc acgcaaaata gcctcgtcgt caatgccctc ttccgtagca
    62641 accgcaaccg cagctgcggc gttcagcgcg ttatgacggc ctggcgcatt cagggtgacg
    62701 cgcatcggct ctttgtcctg gcgcagcagc gtaaagtgcc cctgcgggcc aatctgctga
    62761 taatcttcta cacgcacgtc ggcatcttcg ctgaagccgt aagttgtggt ctgacgtccc
    62821 actcgcggta acaattcgcg gatcaccgga tcatcaacac acatcaccgc acgaccgtaa
    62881 aacggcaggt tgtgcagaaa attaataaaa gtctgtttta aattctcaaa gtcgccctgg
    62941 taggtatcca tgtggtcggc ttcgatattg gtgacaatcg ccaccatcgg ttgcagatgc
    63001 aggaacgatg catcactctc atctgcttcg gcaatcaggt accgaccatg ccccaaacgc
    63061 gcatgaaccc ccgccgcttt taccagcccg ccgttaacga aggttgggtc gagccccgct
    63121 tctgcgtaga tgctggagac catcgcggta gttgtcgttt tgccgtgcgt tcctgcaatg
    63181 gcgatgccat gacgaaaacg cattaactca gccagcattt cggcacgacg gatcaccgga
    63241 atacgcgctt catgagctgc gacaatttcc gggttatcgg cagaaatggc gctggaaaca
    63301 acgaccacgc tggcatcacg tacgttttcc gggcgatggt tgaaataaat cgtcgctccc
    63361 agattcatta actgctgcgt gaccggattt ggcgctaaat cggaaccact gatctgataa
    63421 ccttcattgg ccagaacttc ggcaataccg cccataccgg caccaccgat gccgacaaaa
    63481 tgtatgtgcc gaacgcgacg catttcgggc acgatggaac gcagttttgc caattgttgt
    63541 gtattcattc tttacgccat taacttctta aattcatacg atgcaaaagg catcgctaca
    63601 attacgcccg ggcagcccga ctcacttcat ttgccactcg ctcggtggca tccggaatgg
    63661 atgcagcgcg ggcgcgttct gccatggtta ataaggtttc tcgcgaccac ccggccaggg
    63721 tgttggcgac agcatccacg ctaagctgtg gctgctcgat aattttggct gcgcccgctt
    63781 tttccagcgg tagcgcattc cagtattgct ggcggtcttt atgttgaaac ggcacaaaca
    63841 acgccggtag tcctgccgcg gcgatttcac tcaccgttaa agcaccggag cggcaaacga
    63901 cgacatccgc ccacgcatac gccgccgcca tatcatcaat aaattcagtc actttatgct
    63961 gcggttgccc cgcttcggca tacgcctgtt caacggattg ttgcgaacct ttgccgctct
    64021 gatgccagat agtgactgaa tcacccagtt tcgcagcaac ctgcggcatt gtctggttaa
    64081 gaatgcgtgc gccctgggaa ccacccacta ccagcacacg aaccggacct tcacgtccag
    64141 ccaaacgttg ctgcggcaac ggtagcgcca acacatcggt acgcaccggg ttacccacca
    64201 cttccgcatt ggggaaagca cctggaaacg cctgcatcac tttggtggca atcttcgcca
    64261 gccatttatt ggttaagccc gcaataccgt tttgttcatg aagtacaacc ggaatgccta
    64321 acgaccacgc ggccagacca ccaggacctg atacgtagcc gcccataccg agcaccacgt
    64381 caggtttgta cgctttcata atcgcccgcg cctgacgcca ggcattgaag atacgcagcg
    64441 gcgcagctat cagtgctttt ataccttttc cacgcagacc agagatacga atgaaatcaa
    64501 tttcgatgcc atgttttggc actaagtccg cttccatacg gtcggcagtc cccagccagc
    64561 gaacttgcca accctgagcc attagatggt gcgcaaccgc cagtcccggg aatacatgtc
    64621 caccggttcc gcctgccatc accattaatc gctttccttg agcactcatc gtgaacctcg
    64681 tacaaacgcc tgcgctttct ccagacgcgt ttcataatca atacgcaaca gcatcatgat
    64741 ggctgtcgac ataatcagta agctcgaacc accgtaactg atcagcggca atgtcagacc
    64801 tttggtcggt aacatccccg ccgccgcgcc tacgttaacc agcgcctgga agctaaacca
    64861 gatgccaata gaacaggcga gaaaaccgga aaaacggtgg tcaatttcta atgctttacg
    64921 gccaatcgac atcgcgcgaa aagcgacgaa gaataccatt aaaagtgcca gcaccacacc
    64981 gacatacccc agttcttcgc cgataatggc gaaaataaag tcagtgtgcg cttccggcag
    65041 atactccagt ttttgtacag agttacctaa accttgtccc cacagttcgc cgcgaccaaa
    65101 cgccatcagc gattgcgtta actgatagcc gctgccaaag ggatcttccc acgggttcca
    65161 gaatgcggta acacggcgga tacggtacgg ttcggcgagt atcagcaaca caaccgctga
    65221 aatgcccata ccgataatgg caatgaactg ccacaatttc gctcccgcca ggaacaacat
    65281 cgccagcgta gtcacaaaca acaccaccac cgtaccaagg tctggctgtg ccagcagtaa
    65341 cactgccaac accagaatca cgcccatcgg tttcaggaag ccgcgcaggt tattacgtac
    65401 ttcgtcacct ttacgcacca gatagttggc gatatagcaa aacagcgaca gttttgtcag
    65461 ctcagcaggc tggatacgca gcaaaccgag atcgatccaa cgcgatgccc ctttaaccga
    65521 gctccccact accagcacga tcatcagcag gatgatagat ccgagcagca tcgtggcact
    65581 gtagcgttgc cagaactcca tcggcagacg cagcgtaatg atcgccagaa taaacgccaa
    65641 aatcagatag acaccatcac gcttcgcgaa gaagaacgga tcgttggtta agcgttgccc
    65701 tatgggcatt gacgccgagg tcaccatgat aaagccaatc gccgcgaggc cgaaggtcag
    65761 ccacagtaag gtgcgatcgt acatgatcag gctgtcggta tctttttccc gcgagcccat
    65821 cacccagccc tttagcgccg tggagatcca gaccaggata ctgaatcctg gcaggcgcgg
    65881 cattttcagg cgagggagag ataaacgcat caacctaact ccttcgccag acgggcaaac
    65941 tcattgcctc gttgttcaaa gttcttgaac tgatcaaggc tggcacaggc cggggagagt
    66001 agaaccatat cgcccggctg aacacgcgga gccagcaagc gcatcgcctg ttccatagtt
    66061 tcggtttgtt ctgccacttc cgggcgtagc gccgccagct gcacgccgtc acgaccgaaa
    66121 caatacagac gtacgttatc gccattcagg taacgcgcca gtgggctaaa gtccgccgat
    66181 ttaccatcgc cgcccagcaa caaatgcagc gtgccgtcta cgtgcaggcc attcagcgcc
    66241 gcttccgtac tgccgacgtt ggtcgctttc gaatcgttaa tccaacgtac gccgttatgc
    66301 tccagcacaa cttcaaagcg atgcggcaga ccagtgaatg tggttaacgc tttcaggctg
    66361 ctggcacgcg gtaaccctgc ggcatctgcc agcgccagcg ccgccagcgc attggtgtag
    66421 ttatgctgcc cggaaaattt catttctttc acgttcagca ctttctcgcc tttcacccgc
    66481 agccaggttt cgccctgctg atgattcagg tgatagtcac ccatgttgac gccaaagctg
    66541 acgcagcgtt catccgcacc gcgaatcggc attgttaagg catcatcagc attgacgaca
    66601 caaactttcg cattttcgta aatacgcaat tttgccgcac ggtattgctg taaaccaaac
    66661 ggatagcgat ccatatgatc ttcagtcacg ttcagaatgg tcgctgctac cgcctgtaag
    66721 ctggaggtgg tttccagctg gaagctcgac agttccagta cgtacagttc gcactcatca
    66781 tccagcagca tcaacgcggg caggccaata ttgccaccca caccaacgtt aacccccgcc
    66841 gctttcgcca tttcacccac tagcgtggtg accgtgcttt tgccgttaga accggtaatc
    66901 gccacaatcg gtgcttgtgc ttcgcgacag aacagctcga tatcgccaac gatttcgatt
    66961 ccggcatcag cggcagcgct taaggatgga tgcgccagtg caataccggg actggcgaca
    67021 atcagatctg ccgccatcag ccattcatca ttcagactgc ccgtgtggcg ttctacggct
    67081 tcgggtaatt tatccaggcc aggcggtgtc atacgcgtat ccataacgcg cggcgtcaca
    67141 ccgcgagcga ggaaaaagtc cacgcaggaa agcccggtga ggcccaggcc gataatgaca
    67201 acatttttac cctggtaatc agccatgatt aacgtacctt cagcgttgcc agaccaatca
    67261 ggaccagcat cagcgaaata atccagaaac gcacaatgac gcgcggttcc ggccagcctt
    67321 tcagttcata gtggtgatga atcggtgcca tgcggaaaat acgttgtccg cgcagtttaa
    67381 aggagccgac ctgcaggatg acggaaagcg tttctaccac gaacacgccc cccataatca
    67441 ccagcaggaa ttcctgacgc agcagtacgg cgataatgcc taacgcacca cctaacgcca
    67501 gcgaacctac atcgcccata aagacctgcg ccggataggt gttaaaccac aggaagccca
    67561 gccctgcccc gactatcgcg gtacagacaa taaccagttc cccggcgtgt cgcagatatg
    67621 gtatgtgtaa atagctggca aagttcatgt tgccggtcgc ccacgccacc agcgcaaaac
    67681 caccggcgac aaatacggtc ggcataattg ccagaccgtc aagaccatcg gtcaggttta
    67741 ccgcgttgcc agtacccaca atgacgaagt aagccagcag aatgtagaac agccccagtt
    67801 gcggcatcac atctttaaag aatgggacca ccagctgcgt tgcgggcgtg tctttgccaa
    67861 caaggtacag cgcgaaggcg acacccagcg caatgaccga catccagaaa tacttccaac
    67921 gagcgatcaa ccctttggtg tctttacgca ctactttgcg ataatcatca acaaagccaa
    67981 taacaccgta acctaccagc accaccaaca cgcaccagac gtacggattg gacgggtaag
    68041 cccacagcag tacggagatc acaatcgccg tcaggatcat aatcccgccc atggtcggcg
    68101 taccgcgctt gctgaagtgt gattcaggac cgtcgttacg caccacctga ccaaaggaaa
    68161 gtttttgcaa atgagcaatc atacgcgggc ccatccacaa tgagatgaac agcgcggtca
    68221 gcaggctgac gatggcgcga aacgtcagat aggaaaagac gttaaagccg gaataatatt
    68281 tgaccaaatg ttcggccagc caaactaaca tgtcccattc tcctgtaaag cgcgtactac
    68341 ctcttccatg gcggcactac gtgaaccctt aactaaaatc gtaattacct gttgctcagc
    68401 aatcagtgat ttaagacgcg taattaacgc agttttctca gcaaaatgtt cgccaacgcc
    68461 gctggcggtg ctgatagcat ggctttgttt acccacgctt aacacgcggt caataccagc
    68521 agcttttgcc gcctcgccca cctgtacatg gcaggcttcg ctttcagcgc ccagttccgc
    68581 catatcgccc accaccagca cgcggtagcc cggcatttca gccagtacct gaacagcggc
    68641 ggtcattgaa ccgacattgg cgttgtagga gtcgtcgagc agcaactggt tttctgccag
    68701 ttgaatgggg aacaaacggc ctggaacagc tttcagattt gccagccccg ctttgatagc
    68761 atcaagcgtt gcgcccacgg acatggagag cgcagcggct gccagcgcat tcgcaatatt
    68821 gtgacgcccc ggcaacggca gcagaacatc cacgctaccg gttggggttt gcagggtaaa
    68881 ttccgtaccg tgcgaagtca catggatatt ggtggcggtg aaatcgctgt tggcggcatt
    68941 aggtgagaaa cgccacactt tgcgtgagcc aattacgctc tgccagttca gccagtcgtt
    69001 gttgtcggcg ttcataatgg cgataccgtt ttccggcagg ccgctaaaga tttcaccttt
    69061 cgctttcgcg acacccgcaa gcgagccaaa accttccaga tgcgccgctg ccaggttgtt
    69121 gaccagcgca gcttccgggc gagtcagact cacagtccag acaatttcgc cctgatggtt
    69181 cgcgccaagt tcaataactg cgtaatcgta ttccggcgtt aagcgcaaca gcgtcatcgg
    69241 tacaccgatg tcgttgttga gattgcctgc cgtataaagc gtgttgccgc actggcttaa
    69301 aatcgccgcc gtcatctctt taacggaggt tttgccggag gaacctgtca gagcaaccac
    69361 gcgcgccgga acttgctggc gaacccatgc agccagttca ccaaacgcca gacgcgtatc
    69421 cttgacgatt aactgcggca ggtcgatatc cagcggacgg ctaaccagta gtgcgcctgc
    69481 gccgccagct ttcgcctggt cggcaaaatc atgagcatcg aaacgttcgc ctttcagggc
    69541 aacaaacagg cagcccggcg tcagttttcg ggtatcagtg gttacagcat caagggtgat
    69601 atctgcacct tgcagttcac cgttgagaat gtcggtaagt tggctaaggg ttacgctaat
    69661 catgcaatca cccccagcag acgcgccacc gtgacgcgat cggagtagtc cagacgctga
    69721 ttgccaacaa tctggtaatc ttcatggcct ttgcccgcga ccagcaccac atcgttctct
    69781 ttagcctgca taacggcgca agtcaccgct tcagcacggt cttccatcac tttggcatgt
    69841 ccggcatcta acattcccgc cagaatatcg ttgatgatgg cacgcggctc ttcggtacgc
    69901 ggattatcgt ccgtcaccac cgccacatca gcaaactctt cggcaattgc gcccatcagt
    69961 ggacgcttac ctttatcgcg atcgccacca cagccaaaga cacaccacag cttgcccgca
    70021 cagtgcagac gcgccgcctg taaggctttt tccagtgcat ccggcgtatg cgcgtaatcc
    70081 accaccaccg tcggtttgcc tggcgcagtg aacacttcca tacgtccgca aaccggttgc
    70141 agacgagcag cggttttcag cagatcagcc agtggatagc ccagcgccag cagcgtcgcc
    70201 agcgcgagca gcaggttgct gacgttaaaa gcgcccatca gatggctttc aatttcgcca
    70261 tcgccccaac ttgagctaaa gcgaatagtc gcaccgctgt cgtgatagtt cacttcggtc
    70321 gctttcaacc agcgtccgtg acagttcgga ttgatatgat cttccattga taccgcaact
    70381 gcatccggca gttttgccag ccagcggcgg cctacttcat cgtcggcatt aataatcgcc
    70441 tgaccgcaat gatgttcaga ataaagcagc catttcgcag cttcgtagtg ttccatatca
    70501 ccatgataat caaggtgatc gcggcttaag ttagtaaaga ccgacgccgc aaatttcaat
    70561 gccgccacac ggtgctgtac cagcccgtgg gaggaaactt ccattgcgca aaaagtcgcg
    70621 tcctgatcca ccagccccgc cagctcatgc tgaacatcaa ctgccgaacc ggtggtattt
    70681 tctgtcggga tcactttccc cagcagaccg ttaccaacgg tgcccattac cgcgctggtt
    70741 tcgccaagca gttggctcca ctgcgccagc agctgggtcg tcgtggtttt gccgttggtg
    70801 cccgttacgc ccacgagacg taaattgtca gagggttcat ggtaaaagcg gcccgccagt
    70861 gcagataaac gctcgttgag ctggctgaga tagatgaccg gtacgccgtg catttcacgg
    70921 atttcaccgt cggtcgcctc acctttcgcc tctgcaataa tggcagccac accttgcgct
    70981 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
    71041 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
    71101 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
    71161 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
    71221 ggttcgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
    71281 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
    71341 aggcgcaaca cctgcggtgt aagcaatata tttattgatg tagcgaccgt ccggcccgac
    71401 ctttttcgcg gtaccggttt taatggcaat acgatagcct ttaatcgccg ccttcacgcc
    71461 gccgccaccc ggtagcgcca cgctttccat catatgcacc acagtgcgga caatagattc
    71521 cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
    71581 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggag ttaccattag
    71641 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
    71701 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
    71761 gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt
    71821 actcgacttc tgtaatactc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
    71881 ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc
    71941 acgttgcaac gcggtcatca ccaccatcgg tttaaccgtt gaacccggtt caaacacgtc
    72001 ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta
    72061 tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct
    72121 accggactca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg
    72181 caggcgttca tcaatactca gcgccaggtt gtgcgcagcc tggctgtcag tagaagaaat
    72241 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
    72301 aagccattta tcgaaactct tctcaacgcc ctcaatacct tgactatcga cgttagtaaa
    72361 gccgatgagg tgagcagtca cttcgccgga tggataataa cggcgagatt cttcacgcag
    72421 atgaattccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt ttacctgacg
    72481 cgccagataa ataaagcgcc ctttcgggtt ggcgttaatg cgggcagaaa gctgatccag
    72541 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
    72601 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
    72661 gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
    72721 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
    72781 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
    72841 gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
    72901 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
    72961 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
    73021 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
    73081 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacggtca ccgccgtcaa
    73141 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
    73201 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
    73261 ttctgtcact ctgctgatca tgcgttcgtc ctctctgcaa tacgcagaac tgaactacgg
    73321 gcacgaggat tctcagccac ctcttcttcg cccggcatta atttgcctag tgctcgcagc
    73381 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
    73441 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
    73501 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
    73561 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg
    73621 ggatgtttaa acttatcttt caccggcgtt gcagcagcca cgacttccgc cagttctttg
    73681 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg ctcgggcaat gcgtttggca
    73741 aaacgctctt caccataggt tttcaatacc caggcgatat cggcttcttc tgcggtttgt
    73801 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
    73861 cgcataaagg aaaagccgcg ttcagcatca tcaagttgcg gtgaagagac accaagatcg
    73921 aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg
    73981 gaaaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
    74041 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
    74101 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
    74161 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
    74221 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
    74281 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
    74341 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
    74401 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
    74461 cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc
    74521 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
    74581 ttgaccggca gcgttctcaa gcagctgttc ccgataacgg gtaggcactg ataagcgccc
    74641 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
    74701 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
    74761 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
    74821 ttattttacg tccaacgtgc gaagcaaatt aacgttatga atagctgtaa gaaaaaaacc
    74881 ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
    74941 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt
    75001 ctgtgtagcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc
    75061 ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
    75121 tattgtgaat ttcagctggt gagttaatta actggcgggt ttaaaagcat ttatcaataa
    75181 tagtcaggaa tagtcttatt tactttaagg atattgatgt ccagtcccgt actctacgcg
    75241 ccagagtgaa attcacctgg cgcgtatttt tgttcgcagc ttagctacgg ctgagcacac
    75301 cgcggcgata gagattacgt ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt
    75361 ccaggcttgc caggacaatc tccagcacac gctctgcgac atcgcggtga cgttgagcca
    75421 ctgccagcac cgggcactgt aagaagtcga gcagttcgtt atcgccaaag gtggcaattg
    75481 ccaggtcaga aggcagttta ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca
    75541 acgcaaacga cgttgtgaac agcgcctgcg gcatcggatg cgtttccagc catttttcga
    75601 ataactgggc agccgcctcc cgctcatagc tgttggcata caggaaatgc acttcgcgcg
    75661 gatcatcttt ccaggcagta cggaaacctt gttcacgcag gaagctgaca gaaagctccg
    75721 gcagcgcgcc aagataaagc accgtctcgg cgggaaactt acgtaactct tccgccagca
    75781 tttcggcatc atcctgatcg gcaccaacca cgctggtaaa gtgttcacga tcgagggcgc
    75841 ggtccagcgc gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag
    75901 gaggcaacga cgtcgaaaca ataatggcat cgacctgacg ctgtaaaagg tgctcaatgc
    75961 agcgcatttc gttgtctggc tgatcttctg agcaggcaat cagcagttga taaccccgtt
    76021 gccgcgcctg gcgttcaaga tagttagcga tgcgggtata gctggtgttc tccagatcgg
    76081 ggatcacaag accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg
    76141 ggtggtaatt gtgctcacgc accacagcca tgactttttc aacggttttg tcactcacac
    76201 ggtattgctt cgctttgccg ttaataacat agcttgcagt ggtccgcgac actcccgcca
    76261 gccgagcgat ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc
    76321 attgtactgg tatgggttaa attcattaac atctaagcgc agaaaattaa ctgcggcaac
    76381 tgcttttatt ccaggttccc actgatttcg caaaaaaagc ccaacgtgac atgttgggct
    76441 gtaaattgcg cattgagatc attctcaacg cattatttta tcgccgcgcg aaagtccgac
    76501 cacaccagag cgagcaactt ccacaatttt cgccacatcg cgaatcgatg ctaaaaatgc
    76561 atcaagctta tcgctggtgc ctgctaattg aacggtataa agcgagggtg tgacatcaat
    76621 aatttgccca cggaatattt ccgtattacg tttaacttcg tcacgcccgt aaccgctggc
    76681 ctgaattttc accagcatga tttccctctc aacatgcgcg ccctgcccca actcactcac
    76741 gcgcaagaca tcgaccagct tgtataattg cttttcgatc tgctcaagca ctttttcatc
    76801 gcccacggtc tggatggtca tacgcgataa tgtcggatcg tcggttggcg caacggtcag
    76861 gctttcaatg ttgtagccac gctgggaaaa aaggccaatc acgcgggata acgcgcctga
    76921 ttcattttcg agtaagactg ataatatccg gcgcataatc aggttctctc cgttttgctt
    76981 aaccacattt catccattcc gcccccgcga atctgcatcg ggtagacgtg ctcactgcca
    77041 tcgacggtga catcaacaaa taccaggcga ttattacgca cctgttcaag cgcctcgcta
    77101 agtttgcttt ccagctcttg cggatgagag atctggatcc cgacgtgacc ataggcttcc
    77161 gccagacgga cgaagtctgg taaagactgc atataagatt gtgaatgacg accggaatag
    77221 atcatgtcct gccactgctt caccatcccc aaatagcggt tattaagatt aaccaccagc
    77281 accggcagtt catattgcaa cgctgtagac agttcctgaa tgttcatctg aatactgccg
    77341 tcgccagtga cgcaaaccac ggtttcttct ggcagcgcca ttttgacgcc cagtgccgca
    77401 ggtaaaccaa agcccatagt gccgaggcca ccggagttga tccaacgacg gggtttgtcg
    77461 aatggataat aaagcgcagc aaacatctgg tgctgcccga catcggacgt aacgtaagcg
    77521 ccccccttcg tcaaccgcca aagagtctcg atcaccgcct gcggtttaat cttttcactg
    77581 tgagtgtcat atttcaggca ctgacgagcg cgccactgtt caatctgctg ccaccagtcg
    77641 cggatctcat ccagcggttg atgggcggat tcttgcgaca agagttcaag catttgttcg
    77701 aggacctggc gagcatcccc cacaatcgga atatccgcag tcacggtttt agaaatggaa
    77761 gtaggatcaa tatcgatatg cagaacagtg gcatttgggc agtactttgc cagattgtta
    77821 gtggtgcgat cgtcaaagcg gacaccgaca gcaaaaatca catccgcgtt atgcatcgtc
    77881 atattggctt cgtaggtacc gtgcattccc agcatgccca gtgcctgacg atgcgttgcc
    77941 ggaaacgctc ccagccccat caatgaggaa acaacgggca gatttaacgc ctccaccgtt
    78001 tctttcagct gttgatggca gcccgccgtg attgccccac cgcctacgta gacaaccggt
    78061 ttttttgccg ccaccagcgt ttgcagagcg cgcttaattt gccctttatg tccggtagta
    78121 gtgggattgt aagaacgcat actgaccgac tccggccaga catagggtaa tttgttcgcc
    78181 ggattaagaa tatctttcgg taaatcaacg accactggtc ctgggcgacc acttgccgcc
    78241 agccagaaag cctttttcag cacctgcgga atgtcttccg tttgcttaac cagaaaactg
    78301 tgtttaacca ccggtcgcga aatccccacc atgtcgcact cctgaaaggc atcgtaacct
    78361 atcaacgagg tcgctacctg cccggaaagg acaactaatg gaatggaatc catataagcg
    78421 gtggcgatgc cggtaatcgc attggtcgcc cctggacccg acgttaccag cacgacgccg
    78481 acttccccgg tcgcgcgcgc cagaccatcg gccatatgca ctgccgcctg ctcatgacga
    78541 actaaaacat gaccgatacc acccacggta tgcaatgcat cgtaaatatc aaggactgcg
    78601 cctccgggat aaccgaatac ttgtttaacg ccctgatcga taagcgatcg gacgaccatc
    78661 tcggctccag acaacatctc catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa
    78721 cacattaacc gtccgatctt gcttaagcaa ttggcagcca gagacataaa cgccgggtaa
    78781 taagaataaa caggaggaaa ggtgaaaaga aacgataaaa tcctccatta cgaaggataa
    78841 aaatgataat aaatcggcta aatcccacca cttacagcaa tttctctatt tttttaatta
    78901 atcctaaaat agagagccca cacattcaga ataaaattct acactcaata aaaaatgcag
    78961 aataaaccag acattcatgt ctgacctatt ctgcattcag ttagcgtttg caaattgaga
    79021 ctaattgctc ttccatccac tgatggcctt tatcacgccc ggcagcttca tgccaggaga
    79081 gataacaggt tctgctattt agttttaacg gcagcggtaa tacctgtaat tctaaggatt
    79141 cagcgaactc ttcagccagc caacgcggcg caatagcgac caaatgcgtt tgcgacacca
    79201 cgctaagtac gctcatcatt gccatgccct gatacgcgat actggcttgc ttatctaccg
    79261 tgtcatacca aggttgacta aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt
    79321 cgttataaac atcatgtttc agtaacgggc ccttaattgt tgggtgattt ttgctggcta
    79381 ccagcaccat ttcatcttta aataatggca cgctggtaaa ttcaggacga tggaagtctt
    79441 cataactaat cacaaactcc gtttcctgat aacgcagctg atgttcagtg ttctgattta
    79501 atgaagactt gaacataaca tgtatatttg gcgcaatctg ctcaatgtga ttataaatct
    79561 gcgaggtcag aatactgtct aacgggctgc aaacacaaag atgaaataca cgttcactgc
    79621 tcgcgggttc aaaacctgaa ccaggcaatt cattttgtac tagttgcaat gcctgacgaa
    79681 ctgaaccaaa aagttgaaat gcgcgagcag tcggttgaat accacggcca taacgaacaa
    79741 aaagctcgtc attaaacatc accttcaggc gtgcaacagc gttactgacc gcaggttgcg
    79801 acattcccag aacatgagca gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa
    79861 cggttaataa gttgagatcg accatgcgta gctgtggctt gctgaactcc gccgtctctg
    79921 gatgatctgt ttgtacctct ggcatactta actccactgt cacacttaac tccctttccc
    79981 ttattggaat gcagagaaat aatcctcgag aatatgattt atcatgcata taaaataaga
    80041 aaaaggaaaa tgagtaaaat taggaaaaca taaagatacg ggttcttata aaaacagaat
    80101 ccattgccgt ggaaagcgtc atttaccaca gcataataat ccataatgtt cttgttgcgt
    80161 aaacaatcta atcataacta ccgcgaatac tcaatcatcc acaaaatgga ttaaatgtgc
    80221 tttttaataa ttta
//
LOCUS       DAZFBR010000009        80062 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFBR010000009 DAZFBR010000000
VERSION     DAZFBR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.009"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..589)
                     /locus_tag="ACHJZ2_000739"
     CDS             complement(<1..589)
                     /locus_tag="ACHJZ2_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFO4566908.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSV"
     gene            complement(753..3065)
                     /gene="pbpC"
                     /locus_tag="ACHJZ2_000740"
     CDS             complement(753..3065)
                     /gene="pbpC"
                     /locus_tag="ACHJZ2_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO4566909.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFSFQ"
     gene            complement(3066..8027)
                     /gene="yfhM"
                     /locus_tag="ACHJZ2_000741"
     CDS             complement(3066..8027)
                     /gene="yfhM"
                     /locus_tag="ACHJZ2_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO4566910.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            8234..9079
                     /gene="sseA"
                     /locus_tag="ACHJZ2_000742"
     CDS             8234..9079
                     /gene="sseA"
                     /locus_tag="ACHJZ2_000742"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO4566911.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            9422..9547
                     /gene="timP"
                     /locus_tag="ACHJZ2_000743"
     CDS             9422..9547
                     /gene="timP"
                     /locus_tag="ACHJZ2_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO4566912.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVINNLQSSR"
     gene            complement(9579..10355)
                     /gene="sseB"
                     /locus_tag="ACHJZ2_000744"
     CDS             complement(9579..10355)
                     /gene="sseB"
                     /locus_tag="ACHJZ2_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO4566913.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(10498..11781)
                     /gene="pepB"
                     /locus_tag="ACHJZ2_000745"
     CDS             complement(10498..11781)
                     /gene="pepB"
                     /locus_tag="ACHJZ2_000745"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO4566914.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(11959..12159)
                     /gene="iscX"
                     /locus_tag="ACHJZ2_000746"
     CDS             complement(11959..12159)
                     /gene="iscX"
                     /locus_tag="ACHJZ2_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO4566915.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(12171..12506)
                     /gene="fdx"
                     /locus_tag="ACHJZ2_000747"
     CDS             complement(12171..12506)
                     /gene="fdx"
                     /locus_tag="ACHJZ2_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO4566916.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(12508..14358)
                     /gene="hscA"
                     /locus_tag="ACHJZ2_000748"
     CDS             complement(12508..14358)
                     /gene="hscA"
                     /locus_tag="ACHJZ2_000748"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO4566917.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(14375..14890)
                     /gene="hscB"
                     /locus_tag="ACHJZ2_000749"
     CDS             complement(14375..14890)
                     /gene="hscB"
                     /locus_tag="ACHJZ2_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO4566918.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(14986..15309)
                     /gene="iscA"
                     /locus_tag="ACHJZ2_000750"
     CDS             complement(14986..15309)
                     /gene="iscA"
                     /locus_tag="ACHJZ2_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO4566919.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(15326..15712)
                     /gene="iscU"
                     /locus_tag="ACHJZ2_000751"
     CDS             complement(15326..15712)
                     /gene="iscU"
                     /locus_tag="ACHJZ2_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO4566920.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(15740..16954)
                     /gene="iscS"
                     /locus_tag="ACHJZ2_000752"
     CDS             complement(15740..16954)
                     /gene="iscS"
                     /locus_tag="ACHJZ2_000752"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO4566921.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(17066..17554)
                     /gene="iscR"
                     /locus_tag="ACHJZ2_000753"
     CDS             complement(17066..17554)
                     /gene="iscR"
                     /locus_tag="ACHJZ2_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO4566922.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(17855..18592)
                     /gene="trmJ"
                     /locus_tag="ACHJZ2_000754"
     CDS             complement(17855..18592)
                     /gene="trmJ"
                     /locus_tag="ACHJZ2_000754"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO4566923.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            18711..19514
                     /gene="suhB"
                     /locus_tag="ACHJZ2_000755"
     CDS             18711..19514
                     /gene="suhB"
                     /locus_tag="ACHJZ2_000755"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO4566924.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            19659..20513
                     /gene="yfhR"
                     /locus_tag="ACHJZ2_000756"
     CDS             19659..20513
                     /gene="yfhR"
                     /locus_tag="ACHJZ2_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4566925.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            20704..21984
                     /gene="csiE"
                     /locus_tag="ACHJZ2_000757"
     CDS             20704..21984
                     /gene="csiE"
                     /locus_tag="ACHJZ2_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO4566926.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(21976..23115)
                     /gene="hcaT"
                     /locus_tag="ACHJZ2_000758"
     CDS             complement(21976..23115)
                     /gene="hcaT"
                     /locus_tag="ACHJZ2_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO4566927.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(23275..24165)
                     /gene="hcaR"
                     /locus_tag="ACHJZ2_000759"
     CDS             complement(23275..24165)
                     /gene="hcaR"
                     /locus_tag="ACHJZ2_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO4566928.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            24301..25662
                     /gene="hcaE"
                     /locus_tag="ACHJZ2_000760"
     CDS             24301..25662
                     /gene="hcaE"
                     /locus_tag="ACHJZ2_000760"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO4566929.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSNKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            25659..26177
                     /gene="hcaF"
                     /locus_tag="ACHJZ2_000761"
     CDS             25659..26177
                     /gene="hcaF"
                     /locus_tag="ACHJZ2_000761"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO4566930.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            26177..26497
                     /gene="hcaC"
                     /locus_tag="ACHJZ2_000762"
     CDS             26177..26497
                     /gene="hcaC"
                     /locus_tag="ACHJZ2_000762"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO4566931.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            26494..27306
                     /gene="hcaB"
                     /locus_tag="ACHJZ2_000763"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             26494..27306
                     /gene="hcaB"
                     /locus_tag="ACHJZ2_000763"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO4566932.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            27316..28528
                     /gene="hcaD"
                     /locus_tag="ACHJZ2_000764"
                     /pseudo
     CDS             27316..28528
                     /gene="hcaD"
                     /locus_tag="ACHJZ2_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            28625..29047
                     /gene="yphA"
                     /locus_tag="ACHJZ2_000765"
     CDS             28625..29047
                     /gene="yphA"
                     /locus_tag="ACHJZ2_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4566933.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(29095..29967)
                     /gene="yphB"
                     /locus_tag="ACHJZ2_000766"
     CDS             complement(29095..29967)
                     /gene="yphB"
                     /locus_tag="ACHJZ2_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO4566934.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(29979..31136)
                     /locus_tag="ACHJZ2_000767"
     CDS             complement(29979..31136)
                     /locus_tag="ACHJZ2_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4566935.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALSYEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPV
                     GMMGMMLAKGRGAKRIIGVDMLPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGA
                     DVALDCSGNAAGRLLALQSTADWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSL
                     FHMEKCAHDLTDWKLWPRNAITHRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(31161..32672)
                     /gene="yphE"
                     /locus_tag="ACHJZ2_000768"
     CDS             complement(31161..32672)
                     /gene="yphE"
                     /locus_tag="ACHJZ2_000768"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4566936.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(32695..33678)
                     /gene="yphF"
                     /locus_tag="ACHJZ2_000769"
     CDS             complement(32695..33678)
                     /gene="yphF"
                     /locus_tag="ACHJZ2_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO4566937.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(33775..37056)
                     /gene="yphG"
                     /locus_tag="ACHJZ2_000770"
     CDS             complement(33775..37056)
                     /gene="yphG"
                     /locus_tag="ACHJZ2_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO4566938.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            37174..38367
                     /gene="yphH"
                     /locus_tag="ACHJZ2_000771"
     CDS             37174..38367
                     /gene="yphH"
                     /locus_tag="ACHJZ2_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO4566939.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(38430..39683)
                     /gene="glyA"
                     /locus_tag="ACHJZ2_000772"
     CDS             complement(38430..39683)
                     /gene="glyA"
                     /locus_tag="ACHJZ2_000772"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO4566940.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            40011..41201
                     /gene="hmpA"
                     /locus_tag="ACHJZ2_000773"
     CDS             40011..41201
                     /gene="hmpA"
                     /locus_tag="ACHJZ2_000773"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO4566941.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(41246..41584)
                     /gene="glnB"
                     /locus_tag="ACHJZ2_000774"
     CDS             complement(41246..41584)
                     /gene="glnB"
                     /locus_tag="ACHJZ2_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO4566942.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(41645..42979)
                     /gene="glrR"
                     /locus_tag="ACHJZ2_000775"
     CDS             complement(41645..42979)
                     /gene="glrR"
                     /locus_tag="ACHJZ2_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO4566943.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(42969..43682)
                     /gene="qseG"
                     /locus_tag="ACHJZ2_000776"
     CDS             complement(42969..43682)
                     /gene="qseG"
                     /locus_tag="ACHJZ2_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO4566944.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(43847..45274)
                     /gene="qseE"
                     /locus_tag="ACHJZ2_000777"
     CDS             complement(43847..45274)
                     /gene="qseE"
                     /locus_tag="ACHJZ2_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO4566945.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(45850..49737)
                     /gene="purL"
                     /locus_tag="ACHJZ2_000778"
                     /gene_synonym="purI"
     CDS             complement(45850..49737)
                     /gene="purL"
                     /locus_tag="ACHJZ2_000778"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO4566946.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            49994..51550
                     /gene="mltF"
                     /locus_tag="ACHJZ2_000779"
     CDS             49994..51550
                     /gene="mltF"
                     /locus_tag="ACHJZ2_000779"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO4566947.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQ
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(51547..52050)
                     /gene="tadA"
                     /locus_tag="ACHJZ2_000780"
     CDS             complement(51547..52050)
                     /gene="tadA"
                     /locus_tag="ACHJZ2_000780"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO4566948.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(52108..52743)
                     /gene="yfhb"
                     /locus_tag="ACHJZ2_000781"
     CDS             complement(52108..52743)
                     /gene="yfhb"
                     /locus_tag="ACHJZ2_000781"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO4566949.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            52952..53800
                     /gene="yfhH"
                     /locus_tag="ACHJZ2_000782"
     CDS             52952..53800
                     /gene="yfhH"
                     /locus_tag="ACHJZ2_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO4566950.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            53856..54116
                     /gene="yfhL"
                     /locus_tag="ACHJZ2_000783"
     CDS             53856..54116
                     /gene="yfhL"
                     /locus_tag="ACHJZ2_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO4566951.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(54310..54390)
                     /gene="shoB"
                     /locus_tag="ACHJZ2_000784"
     CDS             complement(54310..54390)
                     /gene="shoB"
                     /locus_tag="ACHJZ2_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO4566952.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(54812..55192)
                     /gene="acpS"
                     /locus_tag="ACHJZ2_000785"
     CDS             complement(54812..55192)
                     /gene="acpS"
                     /locus_tag="ACHJZ2_000785"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO4566953.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(55192..55923)
                     /gene="pdxJ"
                     /locus_tag="ACHJZ2_000786"
     CDS             complement(55192..55923)
                     /gene="pdxJ"
                     /locus_tag="ACHJZ2_000786"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO4566954.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(55935..56663)
                     /gene="recO"
                     /locus_tag="ACHJZ2_000787"
     CDS             complement(55935..56663)
                     /gene="recO"
                     /locus_tag="ACHJZ2_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO4566955.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(56675..57580)
                     /gene="era"
                     /locus_tag="ACHJZ2_000788"
     CDS             complement(56675..57580)
                     /gene="era"
                     /locus_tag="ACHJZ2_000788"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO4566956.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(57577..58257)
                     /gene="rnc"
                     /locus_tag="ACHJZ2_000789"
     CDS             complement(57577..58257)
                     /gene="rnc"
                     /locus_tag="ACHJZ2_000789"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO4566957.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(58530..59504)
                     /gene="lepB"
                     /locus_tag="ACHJZ2_000790"
     CDS             complement(58530..59504)
                     /gene="lepB"
                     /locus_tag="ACHJZ2_000790"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO4566958.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(59520..61319)
                     /gene="lepA"
                     /locus_tag="ACHJZ2_000791"
     CDS             complement(59520..61319)
                     /gene="lepA"
                     /locus_tag="ACHJZ2_000791"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO4566959.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(61517..61996)
                     /gene="rseC"
                     /locus_tag="ACHJZ2_000792"
     CDS             complement(61517..61996)
                     /gene="rseC"
                     /locus_tag="ACHJZ2_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO4566960.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(61993..62949)
                     /gene="rseB"
                     /locus_tag="ACHJZ2_000793"
     CDS             complement(61993..62949)
                     /gene="rseB"
                     /locus_tag="ACHJZ2_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO4566961.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(62949..63587)
                     /gene="rseA"
                     /locus_tag="ACHJZ2_000794"
     CDS             complement(62949..63587)
                     /gene="rseA"
                     /locus_tag="ACHJZ2_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO4566962.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(63620..64195)
                     /gene="rpoE"
                     /locus_tag="ACHJZ2_000795"
     CDS             complement(63620..64195)
                     /gene="rpoE"
                     /locus_tag="ACHJZ2_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO4566963.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVLSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(64192..64347)
                     /gene="rseD"
                     /locus_tag="ACHJZ2_000796"
     CDS             complement(64192..64347)
                     /gene="rseD"
                     /locus_tag="ACHJZ2_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO4566964.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            64603..66225
                     /gene="nadB"
                     /locus_tag="ACHJZ2_000797"
     CDS             64603..66225
                     /gene="nadB"
                     /locus_tag="ACHJZ2_000797"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO4566965.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(66210..66947)
                     /gene="trmN"
                     /locus_tag="ACHJZ2_000798"
     CDS             complement(66210..66947)
                     /gene="trmN"
                     /locus_tag="ACHJZ2_000798"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO4566966.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            67079..68413
                     /gene="srmB"
                     /locus_tag="ACHJZ2_000799"
     CDS             67079..68413
                     /gene="srmB"
                     /locus_tag="ACHJZ2_000799"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO4566967.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(68446..69327)
                     /gene="yfiE"
                     /locus_tag="ACHJZ2_000800"
     CDS             complement(68446..69327)
                     /gene="yfiE"
                     /locus_tag="ACHJZ2_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4566968.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            69430..70017
                     /gene="eamB"
                     /locus_tag="ACHJZ2_000801"
     CDS             69430..70017
                     /gene="eamB"
                     /locus_tag="ACHJZ2_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO4566969.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(70073..70456)
                     /gene="grcA"
                     /locus_tag="ACHJZ2_000802"
     CDS             complement(70073..70456)
                     /gene="grcA"
                     /locus_tag="ACHJZ2_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO4566970.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            70761..71450
                     /gene="ung"
                     /locus_tag="ACHJZ2_000803"
     CDS             70761..71450
                     /gene="ung"
                     /locus_tag="ACHJZ2_000803"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO4566971.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(71498..72535)
                     /gene="yfiF"
                     /locus_tag="ACHJZ2_000804"
     CDS             complement(71498..72535)
                     /gene="yfiF"
                     /locus_tag="ACHJZ2_000804"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4566972.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            72742..73161
                     /gene="trxC"
                     /locus_tag="ACHJZ2_000805"
     CDS             72742..73161
                     /gene="trxC"
                     /locus_tag="ACHJZ2_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO4566973.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            73230..73928
                     /gene="tapT"
                     /locus_tag="ACHJZ2_000806"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             73230..73928
                     /gene="tapT"
                     /locus_tag="ACHJZ2_000806"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO4566974.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            73960..76620
                     /gene="pat"
                     /locus_tag="ACHJZ2_000807"
     CDS             73960..76620
                     /gene="pat"
                     /locus_tag="ACHJZ2_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO4566975.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            76734..78089
                     /gene="pssA"
                     /locus_tag="ACHJZ2_000808"
     CDS             76734..78089
                     /gene="pssA"
                     /locus_tag="ACHJZ2_000808"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO4566976.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            78135..78458
                     /gene="yfiM"
                     /locus_tag="ACHJZ2_000809"
     CDS             78135..78458
                     /gene="yfiM"
                     /locus_tag="ACHJZ2_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO4566977.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(78455..79753)
                     /gene="kgtP"
                     /locus_tag="ACHJZ2_000810"
     CDS             complement(78455..79753)
                     /gene="kgtP"
                     /locus_tag="ACHJZ2_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO4566978.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(79870..>80062)
                     /locus_tag="ACHJZ2_000811"
     CDS             complement(79870..>80062)
                     /locus_tag="ACHJZ2_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566979.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRFPQLAYFHT
                     CPPYQKFILKLLNNHFPAN"
BASE COUNT        19071 a        21741 c        20142 g        19108 t
ORIGIN      
        1 taactgagtg ataatattca agataaccac cggttaaatt taacagccga cgcgccatcg
       61 catcgccaga aaatgttcca ccagaaacag ccgtcccgac atggacataa cgtgaagctg
      121 ggccatattg ttgcgtgatc cttttcaggt tatcggcaat taaggtcgtc gcctcatccc
      181 atgagatacg ctcaaattta ccctcgcctc gtttacctac ccgcttcatc ggatatttca
      241 aacggtctgg atgataaaca aatttacgat agccgcgacc acgaacacag gcgcgcataa
      301 tcggcatttg tggatccaat tcgttatcgg gaagcgtggt aatttgcgtc accacgccgt
      361 cgcgcatatg cgcccgaata tcgcacttac caccgcagtc aaatgtgcta caggtgggca
      421 cgactttatc agctactgga gctgtaactt ctggcgacgc atctggggat tgcgcctttg
      481 cggtagaaga aataaaaggc aaagtgataa gcgcggaagt cgcttgtaaa aaactacgtc
      541 gtgatacttg cggaaatgta atgcgttcct tttcgtctgg cgtatccatc ttgcgttccc
      601 gaaaatgaaa aaataaatat tcacgtattt attcgagaac aatgctgata acaatcaaat
      661 aattagtcgt ttaaaaaaac gtgagattat taaattatta atgcattcca aatataaata
      721 ccataattag attaataaat attttgtata tttcactgaa atgaaaaatg tactgacgcg
      781 atttgcccta cgtcatccat caccagcaat tgataatcac ctttatccgt caaatgcagg
      841 gtcacgttgc gcccgcgttc agttaacggt tcgccattca gaaaccacca gcgttcacct
      901 gcccctccac ttgattgcaa cggcaaagtc gcttccgctg cgcccggtaa acgtttaata
      961 atctcgccat cgcgaacgcc tgtcagctgc agcgggagct gggcatcgtg accgtatggc
     1021 gggcaaattg tcgaggccgg tggtaagcgc acagcgcgac gttctgatgc gggcaaccag
     1081 ggttccagcg gtagcggcca gacatttatc atttcttgtc gcgcttgcgg gcaatcagcg
     1141 gcaacacgtt tgccattttc atccagccag atggggaaac gaatgccatt aatgccttcc
     1201 tgctccggca gtaatagagt tggcggctga ctcccgtcca gcagccaggt tgccaggcgg
     1261 cggcgacagt taccgtcacc ttccggcaaa gactgtccgc ccggccagca aataacgcca
     1321 cgagtgactg agttcgggcg cgggtcttcc ggcagattag cactgcgcga cagtaagata
     1381 ttattgacct gattcaacaa tggtacggcg ctggcaaaac caaactgacc aacaacgggc
     1441 gtgccgtccg gtctgccagt ccagatccca atgacatagc gagcgttaac cccaatcgcc
     1501 caggcgtcac gatagccata gctggtgccc gttttccatg ccagtggggc gacgcgcggc
     1561 aaggcaccat ccggcaaggg tagcgcttca tcagccataa tccggcgaat gatccacgcc
     1621 gctcccgacg acattaaagg ccgttcaagc agcggatcgt caggctgtaa gcgcaatttg
     1681 cctgccttgc cgtggcgagc aaacgcggta tacgctgccg ccatatcttc cagtttcgca
     1741 ccagcgccgc cgagaatgag cgaaagattc ggcgcagcac cgttgggcaa atataacggc
     1801 aatccaacat tgcgtaactt tgccgcaaac cgtttcggtc cataggcttc cagcacctgc
     1861 acagcaggta aattcagcga gcgcaccagc gcctcgctca tgctgatcgg gccatgaaaa
     1921 ccgctgtcaa agttgccagg tcggtaatca ccggtacgcc gggggacgtc ttgcagcagt
     1981 gatgccgggt ggatcaatcc ttcatccagc gccagaccgt aaacaaacgg tttgagcacc
     2041 gatcctggcg atcggatcgc attgaccata tcaacatgac caaagcgtga atcatcgttg
     2101 agatcaaccg aacccaccca gccgcgaaca cgcatatcgg tatgatcaac cacgatcatc
     2161 gccagtgagc tgcgcggtgg taatcgtcct ttccagtttt gcgccagttc ttccagacgt
     2221 cgttgaagac cggcatccag cgtagtaacg attttgtcgc ttttgctttt accgagcatc
     2281 atgcgcgaaa acagcggtgc cagttgcggc atttgtcggg gtgccagcca gatgggttct
     2341 tcccttgact cttttacctg ctcacgggac cacacacctt gcacggccat ccgttcgagc
     2401 actttattac gcgcgacttc ggcacgctcc ggccaacgat ccgggcgaag acggctgggc
     2461 gcttgcggca aaaccgccag cattgccgcc tcggaatagc ttaaattcgc aggcgacttt
     2521 ccgagataag cccaacttgc cgcaccgatc ccctgcaacg taccgccaaa cggagcgcgg
     2581 ttaagataca aggtcagaat ttcacgctta gacagatgcc attccagttg caacgcgcgc
     2641 cagagctggc gaattttgcc gccaaatgtt ttggggtgag gatcaagcag acgagcaacc
     2701 tgcatagtga gcgtgctgcc accggaaata acccgtcccg aagtgagatc ttgccatgct
     2761 gcgcgcgcca ccgagaatgg attcacccca ggatgcttcc agaaccagcg atcttcataa
     2821 ttgatcagcg cttcaaggta acgtggagaa acatcttcga ttgttacagg ataacgccag
     2881 atgccgtcag catcggcgaa gcgccagagc ggcgtaccat cctgcgccac gaccactcgt
     2941 gcgggattga cttcatgcag cggtagaggc cagagtttat ctgctcccca cgctgcaaga
     3001 aaaaggagaa atggcgcagc tgccagcgtt atccagcagc cgcgtttggt taacaagcga
     3061 ggcatttacg gtctgacaat cagcaaatct tcagccgcgc cggtcgcccg ccattgggga
     3121 acatacattg attccaccat cggttgcggt acctgatacg ttcccggcgt caccgcccgc
     3181 gccagataca ccagcgttac cggttggtat tcatcaacgg caaccgccgc cacaaagcga
     3241 tcgtcacgga actcaatgtg cttaatactc gcctgctgca tctggttcag taagttctgc
     3301 acttcgccac cactttgctc caggctggcg ctaccgttcg ccagattctg gttttccagt
     3361 tccagacccg caggcagcag atccacgact aacgcatccg gcacgctgtt actagctttt
     3421 acctgcaacc agaccagcac cagatcgccg ctacgtaacg agtccagcga tttgctctta
     3481 ccatcagtac caagaatatg acgctcaatt tgcagcacat tgttcgccgg taaaggtgcg
     3541 gattgcggat aaccgctggc atccacacgc agccataacg gctgctcgcc actgttggtc
     3601 acctgcaagg tggcaagttg atcgctattc agattgctgt tttgcgcttt ctcgcctgtc
     3661 agcggctcag ctgagaaaga ggtttgcgcc tgccatttac ctggtaaatc ctgaatcgta
     3721 cgggcagcca ggaacaacgc gttactttcc tgcgtcgata gccagcgttc accaaacgcc
     3781 tgctgcgaaa gtgtgttcag cagggagtac tgctcatcgg gtagcagttt gttctcttcc
     3841 agcaaggaga gcatcaacgc gttgtcgcgc agtggactac cgtaatcacc cagccatatc
     3901 cgctcatcac tattacgcgg cgtttttagc gccagcgcaa tcgcttcttc atcacgcgtc
     3961 gcatcaccca tggttttcag cgcaacgcca agttgcagca gcggtaaacc agaagcggca
     4021 tctgcacgat gctcccagat ttcacgcagc gcacccagcg gagccttttg ctgacgggcc
     4081 aacaccagcg cagcgtaaga ctgtacggcg aatttactgg ctttgagatt atccgcgtac
     4141 gggatcgaca tcatgcccgg atcttgtaaa tagcgcagca gacgctcatt accccggtta
     4201 atggcgtctg tcggcacgct gtaaccctgt tcgcctgcgc ggaccaggaa atccatcacg
     4261 taagccgtca gccagtactc ttcgtcaccg tttttatccc acagcgcaaa gccgccgtta
     4321 tcacgttgca tttgcagcaa acgggaaatg ccgatatcga ccgatgcacg gcgtttctca
     4381 tcactgtcgc ctttgatgcc caacgcctgc agttgggctg cgttggtata aagtgacggg
     4441 aacaggccgc tggcggtttg ctcaagacag ccgtacggat acgcttttaa ctctttgata
     4501 taacgtgcga tgttcagcgg tggtttgccg ctcaacaaca attgcccttc cagcgtaaca
     4561 ggcgagaagt tttgcaatcc atccgccgga atcgcccatg tctcaccagg ctgtaacgcc
     4621 gtaccgtaat taaccgtttg tgccgggaac gccggacgga cgccgatttt ccactgctta
     4681 tgctgatcgg caacggtttc acccggtaac gctaacccgc taatggtggc ctgaatttct
     4741 ccatcgccat aacccggcaa tgctcgcacc gggataaaca gcgtagtacg cacacctggc
     4801 gctaattcaa cggctgcggg tgaatcgctg accagttcaa gcaaaccact ggcggtcagg
     4861 gcaacgttca gtttttgcgg tttatcggta agattagtga tatccagcgt cagacgcgag
     4921 ttatcgccac tcgccataaa gcgcggcatg ttcagttcag caatcaccgg tgcggcaact
     4981 atcactttgc tttcgttgct accgaattca tctgccgtcc aggcttgcgc catgacgcgc
     5041 aattcaccgt taaaatcgcc aatcggcagt gtaaccgagc cttcgccctg ttcgttgagc
     5101 gttaccggca gcgcctgctg cgcgacaata ttgacgtgat tgaccggcgg tttaccacca
     5161 cgtttcagct catcaccatc gccaccgaaa cgcagagctg ccagacgccc ctgaccttca
     5221 ataacctgac cgtaaatatc gtaaatgtct gcgccatagc gtttctgacc aaagaacgcc
     5281 tgccacggat ctggcgtgac gtagtcagta atattcagca caccactatc aacggcagac
     5341 accagcacat tcacctgttt aggcttctcg ccatttttag tgctggcttt aattttcacg
     5401 gttaatggtt gattgggacg catttttgct ggtgtttcca gcgccagatc gaggcgacgg
     5461 ttttcatcac caagcggcag atgcaacaca ccaaccgcgc gttttggcgt cgcggagcga
     5521 gatttatcgc caggacgcac caccagcgta ctcaaataaa gatcatgacg attccaggtt
     5581 ttatcgaccg gaatcgtcag atccagccct tgagccggaa catcaatctc ttgccaccac
     5641 agcggccctt cactggactc gaccatcgca taacctttac ccgccgttgg cgcggcgata
     5701 tgcaacttaa tggtatcgcc agggcgataa ccggctttat ccagtttcag cgtgacacgg
     5761 tcgggtcgca ctgcgccgct accgtcgcta ttatcctgcc agctatagcc agcccaaaaa
     5821 cgaacactac tgaccgcttc attcggcgct ttgacttcca gacgataagc gccccactct
     5881 accgggaaac tgaccttgcc ggtttcgtcc gctttcagat ccagagtttg ttcattttca
     5941 atcagatctt tttgatcaaa ctgtgattgc cagccttcat cttctgacca gttccagtag
     6001 taatcgcggc gttcgcgaat caggcgcacc tgcaagcccg acacggcttt tttcacgcct
     6061 tgcgcatcgc tataaacgat gtcaaaagcg gcgttactgc cttcatcaac aatgggctgt
     6121 ttcaccgtgc tgtcagtgcg ataatcgtaa accgatttcg aggcgaactg cggacggatc
     6181 cccggcaatg catcggcagg ccagatagcc tgctcagcgc ggcgcgtcac cgggcgaccg
     6241 cccgattcca gcaaactgcc ctggaaaata acctgcaatg gggaatgcgt ttccttccac
     6301 tggctttctg tagaaacttc gccgcgccct ttatcatcca gcgtcaactg aacttcatcc
     6361 agcgtgcggg aaaggttttc ggcagctata tcgccgaatt caaaaccagg taaggctgac
     6421 acagcctcac gcagtgggcg caggaaaagt tgcccttgca aagtattacc attagcaggc
     6481 gcaccgtaca ggtagtatcc caccacggag aatttcactt catcattcgg cgtaagcggg
     6541 gttttctcac cggtcagatt cagcgccata cgctctggca taaaatcttc gacgtggaaa
     6601 tcccacatcc gatactggtt atcgcccgtg ttagcgcgaa tatgccacat accggttgcc
     6661 gcattgctat cgagtggcca ggtaaagtgg tagaggccat tttccggctg actaacgacg
     6721 ctcctgagta cctgcccatc gggtttaatc acgtctaact tgatgggttg attgggcaac
     6781 gctttaccgt ctgcatcacg cagcaaacca ttgaggatta ccgtttcacc cgggcgataa
     6841 agatcgcgcg ggccaaacat gaaaaactgt ttgctatagc ctggcgcgcc agcaatgtta
     6901 aattctgcta agtccagcgc cggaagtttt aaatcgagta gcgttgtctg accgtcttta
     6961 cgtgccagca ataatgcggc gtttttatca ttttccagct gcacatgccc ctgagcgtca
     7021 ctggttgcct gagtcagagt ctgccctttc tcatttaata aagagacttc aattccttgc
     7081 tgggccgcgc cgttttccag actttgggta aagatatcca gacgattgtg ataacggtga
     7141 gctgaaacgc cgatatcact taacgtaaac agcgtcgcgg gattactgta atcgtaacgt
     7201 ccagcctgat tcatcacagc cagatacacg cccgcctgct gaagcggttt gatatcgccc
     7261 agcggcagca ataatttttc acgggtgtta cgcgcaggat tgagatcaaa ccgtccggtg
     7321 tagaccagat ccgccatctg cagcagtttg tctgactgcc agttcgccag cgaattgcgg
     7381 tattcccatt ggctaatgaa tgctggcaga gattctggct taacgcggaa gaagttaaca
     7441 tcgacattat taacgttgag cgccattacc ggcagccctt caacgacttt accaggcagc
     7501 agcgaaccac ggctggcaaa accgacactg ggttggatgt cgcgggtagt tatagttttt
     7561 tcgtaatctt tactgaaggt tgcgttgttg agtgctttga cctccttgcc aattgtaacg
     7621 atcaaatcac gtttcggttc gaggtggcgt aaacgcagct ctttaagatt atctgatagc
     7681 tcccaggcac catccacttt gccgcttttt ttatcgacga catgaataac gcgtgagaaa
     7741 tcctgatccg ggtcgagagg gatggagaac gtcagcacca gcgtggctgc accatcaagt
     7801 tggacttcag agagatccag caatgtcagc gccttaccgg cactctgttg ggccagtttt
     7861 tgtcgctctg gcgcggagag ttttgctgaa ctcgcgtttt ctgaagaggc cgctttagta
     7921 acttcagaag gcgcatcttt tttcaccgct gttggcgcgt tatcgttgtt gtcgcaccct
     7981 gccagcgcca gcattagcat gcaggcggct acgcgtaact ttttcatttt tcatccctgc
     8041 cacaatggcc cgttagcaac gtcgaataat tattatgcgt gagaatttac gttatgtaat
     8101 tcagtatcac gcccatacaa tccgaatttt agtgagaatt gtggctcaga cagccaaatc
     8161 agcctctttt gcttgtcgct tttcactaaa catccgacaa tttagcctaa ccccggcaaa
     8221 aatggagatg cctatgtcca cgacatggtt tgtaggagcc gactggctcg ccgaacatat
     8281 tgatgacccg gaaattcaga ttatcgatgc ccgcatggcg tcgcctggac aggaagatcg
     8341 taacgttgct caggagtatc tgaatggaca tattcccggc gcagtgtttt ttgatatcga
     8401 agcgctttct gatcacactt ccccgcttcc gcacatgctg ccgcgcccgg aaacgttcgc
     8461 cgtggcgatg cgtgaattag gcgttaacca ggataagcac ctgattgtct atgacgaagg
     8521 taatcttttc tcagccccac gagcatggtg gatgctgcgc acctttggtg tagagaaagt
     8581 gtcgattctg gggggtggac ttgcaggctg gcagcgcgat gatctgttgt tagaagaagg
     8641 tgcagtagag ctgccggaag gagagtttaa cgccgcgttt aatcctgaag cagtggtgaa
     8701 agtaaccgat gtgttattgg caagccatga aaatacggcg caaattattg atgcccgccc
     8761 ggctgcacgt tttaacgcag aagttgatga acctcgccca ggtttacgtc gcggacatat
     8821 tcccggtgca ttgaacgttc cgtggacgga actggtgcgc gaaggcgaac taaaaacgac
     8881 cgatgaactg gatgcgatat tttttggtcg cggcgtcagc tatgacaaac caattatcgt
     8941 cagctgcggc tctggtgtaa cagcagccgt ggttttgttg gcactcgcga cgctggatgt
     9001 gccaaacgtg aaactgtacg acggcgcatg gagtgaatgg ggcgcgcggg cagatttacc
     9061 ggttgagcca ttgaaataag ctttttacag gcaataaaaa accgccgaat ttggcggttt
     9121 tttattgctg gtctggttcg cggcctttcc agcaggttga cttgtgttgc cggagcaacg
     9181 caggcacccc acagtgttga aatactctct tttatgcctg gctgtgtcaa gataataaac
     9241 agagtaatta ttcaaacatg agagtattat tcttaatggc ccttttcgcc gtctcgcaaa
     9301 cgggcgctgg ctttaggaaa gaatgttcca tgaccgtaaa tacaggcacc ccacagtgct
     9361 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
     9421 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt taaccgaggt
     9481 aaataataac cggagtctct ccggcgacaa tttactggtg attaacaacc ttcagagcag
     9541 caggtaagcc cgaatgccgc cctttggggc ggcatatttt agattatccg attctgttta
     9601 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata
     9661 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
     9721 ggtaatgtat cggtcgctac acttcccgcc acctgaataa tttcttcgat atcaccatcg
     9781 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
     9841 aaagcacgct tcacaggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
     9901 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
     9961 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
    10021 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
    10081 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagtgcttct
    10141 aacgaggtaa aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
    10201 agcgcgctat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
    10261 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
    10321 agcaggtctt caagttcatt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
    10381 ggggcacaag tgcctgatgc gccatactta tcaggcctac aagggattca caatttgttg
    10441 aatttgcaat actttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta
    10501 cgccgttaac agattagcaa tcgtgcgcac accaagtccc gtagcacccg cagaccactg
    10561 ttcaaccggc gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata
    10621 gttctcaaca aagtgcgaca ggaagcccgc tgccgtgctc gcgcctgccg gatacgccgc
    10681 gcttccggta ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc
    10741 cagcggcaga cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc
    10801 aagcgcatcg tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc
    10861 cccggtgagg gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag
    10921 accatcggca agcaccaggc gcccttcggc atcagtgttc atcacttcaa cttttttacc
    10981 gttgcgataa gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc
    11041 acagcagagg aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc
    11101 cccggtaacc gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg
    11161 tttgatgctg tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac
    11221 tggcgcttct ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc
    11281 tgaaccgcgt ccgacggtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt
    11341 ggtgatccga taagtcacac gatcgctcgc gacgttgctg atcagatcaa cagcacgctg
    11401 tgccagttgc gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc
    11461 gatgatcatc aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc
    11521 cactttacgc gtgcctttcg gggctttgta accttgccag aatgcccagc atcgatccgc
    11581 atcccagcct tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc
    11641 cgcacgctgg atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt
    11701 attaatgctg taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt
    11761 aatcttcatc gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca
    11821 gccccaggat ttttccgcta aatgattcgc gttgcaggaa ggcggcaagt gagtgaagcc
    11881 ccaggagcat agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg
    11941 tgaagcatga cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag
    12001 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
    12061 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc
    12121 ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca
    12181 cgcgcatggt tgatcgtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc
    12241 gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct
    12301 tcctgctctg agctttctgg aagcgagtca aaaccttcac gaacgatgca gtggcaggtg
    12361 gtgcaagcac aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg
    12421 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca gagatcctga
    12481 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
    12541 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
    12601 tttaatcgct tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc
    12661 ggcagcatcg tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag
    12721 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg
    12781 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
    12841 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
    12901 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
    12961 accgccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
    13021 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
    13081 aaaggtggtg aaatcctgag cgcgggccac tggaatagtg gtattacgcg gaatcacttt
    13141 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcaacag
    13201 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
    13261 gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg
    13321 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcatc
    13381 agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc aataaggttc gttttaccag
    13441 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
    13501 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag
    13561 ttcacgctga acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc
    13621 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
    13681 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
    13741 ataaacggcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt
    13801 cggttcgtta agtaagcgca ggacgtgaag gcccgccaga cgcgccgcgt ctttggtgcc
    13861 ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
    13921 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
    13981 cgggttcagc agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
    14041 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgtc ccatcaggcg
    14101 tttaacagaa ctaattgtgt tggcggtatc gagcgctgca ttagtacgcg cgtcataacc
    14161 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
    14221 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
    14281 caggtcaata ccggccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
    14341 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
    14401 gttgttcggc actgcttcgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg
    14461 ccgcgtccca ggtctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
    14521 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
    14581 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
    14641 gctgctcgct ggcgagatca aagccgtgca aagaaagcaa atattccgcg cgcattaacg
    14701 gatgacgcag cgtttgccag gcctgattaa tggttgcaga ttgctgtacg gcggcgagtt
    14761 gttccgcctg gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
    14821 aacgcaggct cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga
    14881 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
    14941 aacccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
    15001 ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcgttcag
    15061 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
    15121 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
    15181 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gtaggccaaa
    15241 ccctttgcca cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctaagtgt
    15301 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
    15361 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
    15421 caagttcttc agcaatatcg gtgtttttga tagcctgcgc ttcgtcgaga gacttccctt
    15481 tcacccattc ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa
    15541 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc
    15601 cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac
    15661 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
    15721 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
    15781 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
    15841 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
    15901 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
    15961 ttgctgacgt acaggcggaa ccggaagaaa ccgcgagatc tttcagcgcc ataatcagcg
    16021 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
    16081 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
    16141 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca
    16201 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
    16261 cgtgcatttg cgcttcgatg cgtacgcgcg gtttacgacg cacatacagc gcaccgatac
    16321 ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
    16381 ggtcgatagg cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag
    16441 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
    16501 gcatgataga aacaaggatg gtgtcgtcac gcatcgctgc ttcgagttct ttcaggtcga
    16561 taataccgtt acgctgcggt gcgaggtagg tgacttcgaa accttcgcgc tccagctgac
    16621 ggcaggtatc caataccgct ttgtgttcgg ttttgctggt gattatgtgc ttgccttttt
    16681 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agactcggtt gcaccagagg
    16741 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
    16801 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg
    16861 ttccgtccat cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
    16921 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
    16981 aatgcaagga accaggctac cggctggatg tacgaccgtg tttacggagc gcgatacgcc
    17041 ccggcctgat tctgaatact ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg
    17101 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
    17161 tgttaaccag ttcgcctaaa gtaatgttat tgagaaaacc ggtaagacgg tcgctcaaat
    17221 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac
    17281 aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
    17341 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
    17401 ttttacgcag acgagaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
    17461 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
    17521 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
    17581 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
    17641 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
    17701 ccattttttt atcgtggcgt ttgtacctgt tatccttcca gaccatgggc gggttgaggg
    17761 caattattcg cattagcgca ggcctgttcc cctcacccta accctctccc cgaaggggcg
    17821 aggggaccgt ccgtgcactt atctggctta gccctcaggc cttattacct ttattctgct
    17881 gctctataga agccagaatc ccgcgcagga tattcaattc ctggctttcc gggcgcgcac
    17941 gggtaaacag acggcgcaat ttattcatca cctgccccgg atggttttca cggataaaac
    18001 cggttgccag cagcgtttgt tccagatgac cataaaaacg ctccagatca tcgaccagcg
    18061 gatacggcgt ctcttcatgc tcgacctgct cgccattttc ctgagttgcc agccaggcca
    18121 tacgcacttc ataggcaata acctgaaccg ccatcgccag gttcagcgag ctgtattcag
    18181 ggttagcggc aatcgcgaca tgataatgac acttctgcaa ctcttcattg gtcaggccga
    18241 cacgttcgcg accaaacacc agcgccaccg gggtatttgc cgcttcagca acgcttttca
    18301 gaccgcattc gcgcgggtcg agcatcggcc acggcagcgt gcgggaacgt gcgctggtgc
    18361 ccaccaccag gctacaaccg gctaacgctt cgtcgagcgt atcgacgatg tgagcattac
    18421 caatcacatc gctggccccc gccgccaggg caatcgcctg ggagtcaggt ttcaccagtg
    18481 gattaaccag ccacagattg gttaatccca ttgttttcat ggcacgggca acagaaccca
    18541 tattgccggt gtgtgacgtc tccaccagca caattcgaat attttgcagc attgtctttc
    18601 ttcgtctaaa gattattcgc gcatcttatc ataaaacgaa gacagatgcc gatctcgctg
    18661 ctatactctg cgccgttttc ccgttcttta acatccagtg agagagaccg atgcatccga
    18721 tgctgaacat cgccgtgcgc gcagcgcgca aggcgggtaa tttgattgcc aaaaactatg
    18781 aaaccccgga cgctgtagaa gcgagccaga aaggcagtaa cgacttcgtg accaacgtag
    18841 ataaagctgc cgaagcggtg attatcgaca cgattcgtaa atcttaccca cagcacacca
    18901 tcatcaccga agaaagcggt gaacttgaag gtactgatca ggatgttcaa tgggttatcg
    18961 atccactgga tggcactacc aactttatca aacgtctgcc gcacttcgcg gtatctatcg
    19021 ccgttcgtat caaaggccgc accgaagttg ctgtggtata cgatcctatg cgtaacgaac
    19081 tgttcaccgc cactcgcggt cagggcgcac agctgaacgg ctaccgtctg cgcggcagca
    19141 ccgctcgcga tctcgacggt actattctgg cgaccggctt cccgttcaaa gcaaaacagt
    19201 acgccactac ctacatcaac atcgtcggca aactgttcaa cgaatgtgca gacttccgtc
    19261 gtaccggttc tgcggcgctg gatctggctt acgtcgctgc gggtcgtgtt gacggtttct
    19321 ttgaaatcgg tctgcgcccg tgggacttcg ctgcaggcga gctgcttgtt cgtgaagcgg
    19381 gcggcatcgt cagcgacttc accggtggtc ataactatat gctgaccggt aacatcgttg
    19441 ctggtaaccc gcgtgttgtt aaagccatgc tggcgaacat gcgtgacgag ttaagcgacg
    19501 ctctgaagcg ttaatgactc aggcgggtga tatcactcac ccgccctcgc ctttcaggcg
    19561 ctattccgaa atacttcctc accgctttac tttctttcgt cactctccca ccatctttcc
    19621 ccgattaatg gatgataaag aagtcgcaag gaaaggttat ggcactgcca gtgaacaaac
    19681 gcgttcccaa aattctgttt attctctttg ttgttgcctt ctgcgtttat ttagtgccgc
    19741 gcgttgccat caacttcttc tattatcctg acgataaaat ttacggtccc gacccctggt
    19801 cggcggaatc cgtcgaattt acggctaagg acggtactcg tctgcaaggc tggtttattc
    19861 cttcttcgac gggccctgct gacaacgcca tcgcaaccat cattcatgct cacggcaatg
    19921 ccggaaatat gtccgcccac tggccgctgg tcagttggtt acccgagcgt aatttcaacg
    19981 tttttatgtt tgattatcgc gggtttggta aatcaaaagg cacgccgtcc caggccggat
    20041 tgctggacga tacgcaaagc gccatcaatg tggtgcgcca tcgcagtgat gtaaacccac
    20101 aacggctggt gctgttcggg cagagtattg gcggggcgaa tattcttgct gttattggtc
    20161 agggtgatcg tgaaggcata cgtgcagtga tcctcgactc cacatttgcc tcttatgcaa
    20221 ccatcgccaa ccagatgatc cccggcagtg gctacttact tgatgagagt tacagcggcg
    20281 aaaattatat cgccagcgtc agcccgatcc cgcttttact cattcacggt aaagctgatc
    20341 acgttatccc atggcagcac agcgaaaagt tgtatagcct ggcaaaagag ccaaaacggt
    20401 tgatcctaat cccggatggc gaacacattg atgctttttc cgatcgtcac ggcgacgttt
    20461 atcgcgaaca gatggtgaac tttatcctta gcgcgttgaa tccgcagaac taacccatga
    20521 tcgctagcac gataatcatt cacaaaatca ccttaagaca tgctaatcca ctggtcagga
    20581 cagtttaaga tgagaaaaat tctgtgacgc ttgccaacat ttctgatgat tagcattccc
    20641 ttcgccattt ccctgagcaa actttagcta ttcttatcaa ttatgcttat gggagatcta
    20701 cagatgatgc ctacgcttgc tccaccatct gtcctttcgg ctccccagcg ccgctgccag
    20761 atcttgctga cgctctttca gccggggttg accgccacca tggcaacctt cagcgagctt
    20821 aatggtgtgg atgatgatat tgccagtctt gatatcagcg aaacaggacg ggagatcctg
    20881 cgctatcatc aactcacact gacgactggt tatgacggta gctaccgggt tgaaggtaca
    20941 gtgcttaacc aacgtttgtg tttatttcac tggctacgac gtggtttccg tctgtgtccg
    21001 tcatttatta ccagccaatt cacccccgcc ctgaagagtg aactgaagcg gcgcggaatt
    21061 gcgcgtaact tttacgacga taccaatcta caagcgttag tgaatctctg ctcccgacgg
    21121 ctgcaaaaac gctttgaatc gcgcgatatt catttcctgt gtctgtatct gcaatattgt
    21181 ttgctgcagc accacgctgg aattacgcct cagtttaatc cgctccaacg tcgctgggcg
    21241 gaatcctgcc ttgaatttca ggtagcgcag gaaattggac gccactggca gcgtcgggcg
    21301 ctccagcctg taccacctga tgagccactg tttatggcac tacttttttc catgttgcgg
    21361 gttcccgatc cattgcggga tgcgcatcag cgggacagac aattgcgtca gtctatcaaa
    21421 cgtctggtaa accattttcg tgagctggga aatgttcgtt tttatgatga acaggggtta
    21481 tgtgatcagc tttataccca cctcgcccag gcgttaaatc gcagtttgtt tgccatcggt
    21541 attgataata ccctgccgga agagttcgcc agactgtatc cacgcctggt acgcaccacc
    21601 cgcgcggcgc tggccggatt tgaaagtgaa tacggcgtcc atctttctga tgaggaaagt
    21661 ggtctggtcg cggtgatttt cggtgcctgg ctaatgcagg aaaacgatct gcatgaaaaa
    21721 cagattattc tactgaccgg gaatgatagc gagcgagaag cgcagattga gcaacagtta
    21781 cgcgaactaa cgttactgcc gctcaacatt aaacatatgt cggtaaaggc atttttgcag
    21841 acaggcgctc cgcgtggcgc ggcactgatt attgctcctt ataccatgcc gttaccgctc
    21901 ttttcaccac cgctgatcta tacggacctg acactgacaa cacatcaaca ggagcagatc
    21961 cgcaaaatgc tcgaatcagc atgagggaac aactttcggg cgcaaaaaca ttgccggaag
    22021 cgccaccagc gccatcaccc agaacacgcc gtggcccaga tattgataca ggaaaccggc
    22081 gaaaacggtc atgatagcga tactgccgcc catcgcgacg gcagagtaaa ccgcctgtaa
    22141 acggatgact tcgctaccct ggcgagcagc aatatagcgc atggcggcca ggtggcagac
    22201 cgtgaaggtg ccgcaatgca gaatttgcac cactatcaac cacggcaacg ccgtagttgc
    22261 tcccataatg ccccagcgca ctacgccgca aatcgctgag atcaacagca tatcgcgtgc
    22321 actacaacgg cggaaaagtt tattactcag tgcaaagata atgacttccg ccaccacgcc
    22381 cagcgaccac aaatacccca ccgccgaggc cgagtagcca gctgcctgcc agtaaatggc
    22441 gctaaaaccg taataggccg catgtgcccc ctgcaataaa caaacgcagg ccagaaagcg
    22501 ccagttctgg cgaaccagcg ccaaccacgc tgaccaaccg gtgctctcct gctggcggct
    22561 tgccccttgt ggctgaatcg tcggacggat gagaaagccg agcagcatgg atgccacgcc
    22621 caacgtcaac agcacgagga tcacccgata atcaaacata gtgaccagtt tgcccgtcag
    22681 cgccgagcca atgacaaacg ccaccgagcc ccacagtcgc actttgccgt aatcaagcgg
    22741 gaactgcttt tgccacgtat tcgccagtgc atcggtcaac ggtaccagcg gtgagaaaaa
    22801 gaggttaaag ccaatcatca ccagcatcag ccacgctacg tgcgcccccg cccagaaggc
    22861 gacagcaaag agaagtgtca gcagtgccag cacgcgcaag gcggaaatca ggcgagaagg
    22921 atcgctgacg cggggcgcga tgagcaaact cccgagaaaa cgggcaacca gacctgcccc
    22981 caataacagg ccgatggttt ctggcgttaa accaatccct ttaagccaga cgctccagaa
    23041 aggtagaaaa atgccgtaac taaaaaagta tgtgaaatag ccgagcgcca accagcgcgt
    23101 ggattgcaaa accatgagtc cctcccgtca ggaggcgtta gtctggagtt aatctaatgt
    23161 tggtgcaagt tgaaaaataa gaatattcga gcctgcttcc cgatgtctgt aacgccagtt
    23221 cacaagcact caaaaactgc gatccgtgac gcatgttgca aagataatgc gtaattatgc
    23281 cgttacgctt gccaaacgtt cctgcacaat ggcgatgaaa tcgcgtaatg ccggcttcat
    23341 ctcccctttc ttccacgcca tcagtaaagc aatggaaggc acatttccgg caatgggacg
    23401 aaaaacaacc tgcccggtat taaaattatt catataaccg ggtatcaaag tgacgcccag
    23461 ccccatgccc accagattca tggtcaccag aatattcgtt gccacctgga cgatatttgg
    23521 ctggctgttt tcttgtgcaa accacgcttt aacgatcggc gcaagcgaac cggaatacgc
    23581 cggatcggta ctgacgaaat tcacgccatc cagttgcgcg gcggtgatct ctttttcatg
    23641 tgctaaaggg tgatcaaccg gtaacaccac cactaatggt tcgtcaaaaa gctccagata
    23701 atcaatctcc gggctataaa caggatggcg cattaagccg acgtcgagtt caccacggcg
    23761 aattttttcc tcctgttgcg tggtgattaa actcaccagc tcaatcaagg tgtctggctg
    23821 tctgagacga aacatcggta atacttttgg cagtaaattc acttccgccg atggcacaaa
    23881 gccaatggtt aattgtctgt cttcctgaac aattttccgc gcccgtaatt tggcattttc
    23941 cgcttgttcg aggattgcca gcgcgtcctg gagaaaacat tctcccgccg ccgtcaacgc
    24001 gactttgcgc ttatccctca ccagtaacgg aacaccgaca cagttttcaa gatcgcggat
    24061 ctggctgctt aacgaaggct gtgaggtatg cagtttttcc gccgcacggg taaagttcag
    24121 tgcctgcgcc actgcgacga aatagcgtaa atgccgtagt tccatcacct tccccttgtt
    24181 atcgaaaaaa cgtctcagct ggtagaaatt aactatttca catattagca accaaccagc
    24241 aacatcctta tgacacaaaa atagaaggtc aatacatctt atctttcagg attaaaaaat
    24301 atgaccacac cctcagattt gaacatttac caactgattg acacccaaaa cggtcgggtc
    24361 actccgcgta tttataccga cccggacatt taccaactgg agcttgagcg tattttcggt
    24421 cgttgctggc tatttctcgc ccacgaaagc cagatcccaa aacccggtga tttctttaac
    24481 acctacatgg gagaagatgc agttgtcgtg gtgcgccaga aagacggcag catcaaggcg
    24541 tttctcaacc aatgccgcca ccgggccatg cgtgtgagtt atgcagattg cggcaacact
    24601 cgcgccttta cctgcccgta tcacggctgg tcttatggca ttaacggcga gttgatcgat
    24661 gtaccgctgg aacctcgcgc ctacccacaa gggttgtgta aatcccactg ggggctaaac
    24721 gaagttcctt gtgtggagag ttataaaggg ctgatttttg gcaactggga taccagcgca
    24781 ccgggcctgc gtgattacct gggtgacatt gcctggtatc tggatggcat gctggatcgt
    24841 cgcgaaggcg gcaccgaaat tgtcggcggc gtacaaaagt gggtgatcaa ctgcaactgg
    24901 aaattcccgg cagagcagtt cgccagtgac cagtatcatg ctctgttcag ccatgcttct
    24961 gccgttcagg tattaggggc gaaagatgat ggcagcaata agcgcctcgg tgatggacaa
    25021 accgcccgcc cggtgtggga aaccgccaaa gatgccctgc aatttggtca ggatggtcac
    25081 ggcagcggct tcttctttac tgaaaaacca gatgctaatg tctgggtcga tggcgcagtt
    25141 tccagctatt accgcgaaac ctatgccgaa gctgaacaac gtttaggtga agttcgcgcc
    25201 ctgcgcctgg cgggtcataa caatattttc ccgacacttt cctggctcaa cggcactgcc
    25261 acactccgcg tctggcatcc gcgcggccct gatcaagttg aagtgtgggc gttctgtatt
    25321 actgacaaag ccgcctccga tgaagttaaa gccgcttttg aaaacagcgc cactcgtgct
    25381 tttggtcctg ctggttttct cgagcaggat gactcggaga actggtgtga aatccagaaa
    25441 ttgcttaaag gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg gcttggtcag
    25501 gaaaagcgcc gcgacgacgg cattcctggc attactaact atatcttttc agaaactgct
    25561 gctcgtggaa tgtaccaacg ctgggccgat cttctgagta gcgaaagctg gcaggaagtg
    25621 ctcgataaaa ccgccgctta ccagcaggag gtgatgaaat gagtgcgcaa gtttcactag
    25681 agttacatca ccgcattagc cagtttctct ttcacgaagc cagcttactg gacgactgga
    25741 aatttcgtga ctggctggca cagctcgacg aagagatttg ttacaccatg cgcaccacgg
    25801 tgaacgcgca aacgcgcgac cgccgcaaag gtgttcagcc gccgacaacc tggattttta
    25861 atgacaccaa agaccagctg gagcggcgaa tcgcccgtct ggaaacgggc atggcatggg
    25921 cagaagagcc gccgtcacgc acccgccact taatcagcaa ctgccagata agcgaaaccg
    25981 acatcccaaa cgtatttgct gttcgggtga attatctgct ttatcgggca caaaaagagc
    26041 gcgatgaaac gttctatgtc gggactcgtt ttgacaaagt tcgccgtctg gaagatgaca
    26101 actggcgctt gctggaacgg gatatcgtcc tggatcaagc ggtaatcact tcccataacc
    26161 tgagtgtact gttctgatga atcgaattta tgcgtgtccc gttgcggatg tgccggaggg
    26221 tgaggctctc cggatcgata cctcgcccgt catcgccttg ttcaacgttg gcggcgagtt
    26281 ttatgccatt aacgatcgtt gcagccatgg taatgcgtcg atgtcagaag ggtatctgga
    26341 agatgacgcc acggtggagt gcccgctaca cgccgccagt ttttgcctga aaacggggaa
    26401 agcgttatgc ctgcccgcca ccgatccgct caccacttat ccagtacacg ttgaaggtgg
    26461 tgacattttc atcgacttac cggaggcgca gccatgagcg atctgcataa cgagtccatt
    26521 tttattaccg gcggcggatc gggattaggg ctggcgctgg tcgagcgatt tatcgaagaa
    26581 ggcgcgcagg ttgccacgct ggaactgtcg gcggcgaaag tcgccagtct gcgtcagcga
    26641 tttggtgaac atattctggc ggtggaaggc aacgtgacct gttatgccga ttatcaacgc
    26701 gcggtcgatc agatcctgac tcgttccggc aagctggatt gttttatcgg caatgcgggc
    26761 atctgggatc acaatgcctc actggttaat actcccgcag agacgctcga aaccggcttc
    26821 cacgagctgt ttaacgtcaa cgtactcggt tacctgctgg gtgcaaaagc ctgcgctccg
    26881 gcgttaatcg ccggtgaagg cagcatgatt ttcacactat caaatgccgc ctggtatccc
    26941 ggcggcggtg gcccgctgta caccgccagt aaacatgccg caaccgggct tattcgccaa
    27001 ctggcttatg aactggctcc gaaagtacgg gtgaatggcg tcggcccttg tggtatggcc
    27061 agcgacctgc gcggcccaca ggcgctcggg caaagtgaaa cctcaataat gcagtctctg
    27121 acgccggaga aaattgccgc cattttaccg ctgcaatttt tcccgcaacc ggctgatttt
    27181 acgggtccgt atgtgatgtt ggcatcgcgg cgcaataatc gcgcattaag cggtgtgatg
    27241 atcaacgctg atgcgggttt agcaattcgc ggcattcgcc acgtagcggc tgggttggat
    27301 ctttaaggaa gtacgatgaa agaaaaaacg atcattattg tcgggggcgg gcaagcggcg
    27361 gcaatggctg cggcctcgct acgccagcaa gggttcaccg gtgagctgca tctgttttcc
    27421 gatgagcaac atcttcctta tgaacgccct ccgctctcga aatccatgtt gctggaagat
    27481 tccccacagt tgcagtctgt gttacccgct cactggtggc aggaaaacaa tgttcatctg
    27541 cattccggtg taaccatcaa aacattgggc cgcgacacac gagagttagt gttagccaac
    27601 ggcgaaagct ggcactggga tcagcttttt atagcaaccg gcgcggcagc tcgaccgctg
    27661 ccgttgcttg atgcactggg agaacgctgc tttaccctgc gccatgccgg tgatgccgcc
    27721 agactgcgag aagttctgca gcccgaacgg tcagtcgtga ttgtcggtgc cggaactatt
    27781 ggtctggaac tggctgccag cgccacgcag cgcagcgcag cgcagatgta aggtgacagt
    27841 gattgaactg gcggcaaccg tcatgggccg taatgcacca ccgcccgtgc aacgctatct
    27901 tttacagcgc catcagcagg ctggtgtgcg cattctgctc aataatgcca ttgaacatgt
    27961 ggtcgatggt gaaaaagtag aactgacgct gcaaagtggc gagacgcttc aggccgatgt
    28021 ggtgatttac ggtattggta tcagcgccaa cgaccaactg gctcgcgagg ccaaccttga
    28081 tactaccaat ggcattgtca ttgatgaggc ttgccgcacc tgcgatcccg cgatctttgc
    28141 cggtggcgat gtggcaatca ctcgtcttga taatggtgca ctacaccgct gcgaaagctg
    28201 ggaaaacgcc aataaccatg cgcaaattgc cgctgccgca atgttagggc taccgcttcc
    28261 gctactgccg ccgccgtggt tctggagtga tcagtacagt gataacttac agtttattgg
    28321 cgatatgcgt ggcgatgact ggctttgtcg tggcaacccg gaaacccaga aagcgatttg
    28381 gtttaatctg caaaacggcg tgcttatcgg tgcggtaacg ctgaatcagg gacgtgagat
    28441 tcgctcaatc cgcaaatgga tccagagcgg caaaacgttt gatgcgaaac agctgacaga
    28501 tgagaacatc gcgcttaaat cactgtaacc aggataatta gcgaatatct caacgcctgg
    28561 ggcgtggcga ggtaccagag tgtgtattac gtttaaatca cattatcttg caaagggatt
    28621 ggttatgaac tcactacgtt attttgattt tggtgctgcc cgccccgttt tgttattaat
    28681 tgcccgtatc gccgtggtcc taattttcat tatttttggt tttcccaaaa tgatgggctt
    28741 tgacggtacg gtccaatata tggcctcgct gggcgcaccg atgccgatgc tggcagcgat
    28801 tattgcggta gttatggaag tgcccgccgc gatattaatc gtgcttggct ttttcacccg
    28861 tccgctggcg gtactgttta ttttctacac gctgggtacg gcggtgattg gtcaccatta
    28921 ctgggatatg accggcgatg ccgtcgggcc aaatatgatt aatttctgga agaatgtcag
    28981 tatcgctggc gcgttcttgc tattggcaat taccgggccg ggggcaattt ctctcgatcg
    29041 ccgttaggaa agatgccgga tgcggcgtga acgccttatc cggcatttta taaattacag
    29101 ccactctacc cgcaacgaca tctctgaggt tgttgattcc cccggcgcaa gcgcaatgag
    29161 gtcaccccct tccgggcgat gatggtcatc cggcgcatgg ctcatcggct ccagacagaa
    29221 gaaatcaaac gcatatcctt tatcaaacgc cgggtcggaa acaaagatga aataacacgg
    29281 tgcaggtggc gtcgtttcta tgatgatggc ataaccctcc tgcggctgct cgatgcgggc
    29341 ctgaccattc catccggcaa aaccattgtt cacccactgg cgcggcaacg gcgctagctg
    29401 gttaaaatcc agttcttgcg gtagctgctc gcaaaactca cccgccagcc actgctcccg
    29461 ctccagccag taaccgctcg cctgtgcctg aatccgcgtt tgcggcgaca acggaaaata
    29521 aggatgccag ccggtaccaa atggcagcgt ctctgcccct tgattggtga cagagagcgt
    29581 caccgtcagc gtatccgccg ttaaatgaaa cgcctgactt acccgatagt gatagacacc
    29641 gctgcgatgt tcatacacca gacacaaact atcatcgctg tgcgagacac attgccactc
    29701 gcccagccag ccatcgccgt gtagatagtg cgcatcccac tcaacattcg gttgcagttg
    29761 atactcacgc ccctgccaga caaaacggtt gccgctcacc cggtttgcaa atggcaccag
    29821 cggaaaacaa gaggcatcgg ttgccacacc gcttttttta ccaggacgta agagcggcgt
    29881 cgtatcgcgc cagaagcctt cgattacgcc gccctggtca gaaacgccca gctttagcga
    29941 cccgtgggat aaggtataga tggtcatccg ctaactcctt aatccgggaa gttaatcaca
    30001 actttcccgc atttgccgct cgccatcagc gcataggcat cacctgcctg ttccagcgag
    30061 aagcgatggg taatggcgtt acgcggccac agcttccagt cagtcaaatc gtgggcgcat
    30121 ttttccatat ggaacagact ggtcacccag gagccaataa tccgccgttg atggtgcatc
    30181 agatcggcgc tgacctcgaa ttccactttt ccggtttcac caatgtaaac cacccgcccc
    30241 cagtcagcgg tggattgcag tgccagtaag cgacctgcgg cattaccgga acaatcaagc
    30301 gcaacatccg cgccgccgtg agtgagttcg gcgattatct gcggcagacc ttcagtggtc
    30361 gctaaatagc cgtgatccat cactcctaac tgttttgcca tcgccagacg ttccggcagc
    30421 atatcaacgc cgataatccg ttttgcaccg cgacctttcg ccagcatcat ccccatcatg
    30481 ccgactggcc ccagaccgac caccagcacg ttatcactgc cggaaacttc gccgcgcaaa
    30541 atcccttcat aggcagtgcc aacgccgcaa ctgataaacg caccatcttc gtagctcagc
    30601 gcggataccg gcacaccgag aaattgtccg cccagccagt ccagcacctc gttttcgtca
    30661 atcggcactg gcagagcgtt ggtcataaac aagcccattc cgcgcagggc gctccacagc
    30721 cccagcgtgg ccacgaaact tggcacgtta aacacgccac gcagcacccc ggcgagcgtt
    30781 cccatcagcg cgcctaacag caacaccagc agacaagcaa tcgccaacgg aacttcaaat
    30841 tgcagcaaaa atgccaggca taccgagaca aaagccacca tcggcccaac gctgacatca
    30901 atttcaccgg agataataat cagcgtcatc gcccaggcgg caatcccaat ggtggcggca
    30961 tcgcgcagca cgttcatctg gttattcaat gagataaagc caggcgcgtt cagggagaag
    31021 accagataaa gaatggcaat caccaccagc aaaccgatct cattaatatg gtgactgaca
    31081 aattgtttga gcgagacgct cttgccctgc ggcaatggta atgacgaagc agacatcttc
    31141 aggttcctcg gtaaaattca tcagtgcaca gacagaatgg cggacatcag ctcatccaca
    31201 ttgaccggag cgtgaaactc ctgcgagaac gtgccgtgct gtaataacag gatgcggtca
    31261 cacaccaacg gcaactcctc cacttcactg gagataaaca ccacgctttt tccttcggca
    31321 gccagctcac ggacaatacg gtaaatctgc tgtttggctt cgatatcgac gccgcgcgtt
    31381 ggctcgtcga gcaacaaaat ctggctggcg gcatagaccc aacgaccgat cactactttt
    31441 tgctgattgc cgccagaaag cgtgccgatg ggcgtttcgc tactggcggc cttgaccgtc
    31501 atccgctgca tcacttcttc ggtcaggcgg cggatggtgg accattgcag cactccgttg
    31561 gcgctgattt tttgccgatt ggtcagcact gtattttcgt caacgcccaa ccagggaatg
    31621 atccccgctt ctttgcggtt ttctggcgta tagccaatgc cgcgtttcag catgtcgccg
    31681 taatcggggc gcgtgatttt ctcgccgttg ataacaattt cgccctgttc atacgtctcc
    31741 agcccaacaa tcgccttcag caattcactg cgccctgccc ccagcagacc agcaatgccg
    31801 agcacttcgc cacgacgtag cgtaaagctg atatcctcca gcttgggctt atggcgtaac
    31861 gcacggactt ccagcacggc ctgatccaca atttcctgag gtgcaaccgg cgcaatatca
    31921 acgtgatcgc gccctagcat tagcgacaca atatgatgcg tggaagtgtt ttcgagcatc
    31981 acatcgccag ccacctgccc gtcgcgcata acggtggcac tggaggcaat gcggcgaatt
    32041 tcttccatcc ggtggctgac ataaatcacc gccacgccca gtgctgacat ctttttcacc
    32101 gctctgatca ccagttcaac ttccgcactc gcaagcgaac tggtaggttc atcaagaatg
    32161 accacgcgcg gctcgccctt catcacccgc gcaatttcca ccagctgctt atgcgccggg
    32221 cttagtgttg aaacaagttg ttcaggacta acgtcaacgc ccagcgcctg taagcaacgt
    32281 tgggcatcct gcgccatttg caggtaatca atcatgccgt tgcggcgggg ccactgaccg
    32341 aggcagaggt tttccgccac cgtcagccct tccaccagac ttaactcctg ataaaccgcg
    32401 cgtaccccca gttcagcggc acggcgagtc agcgtagctt cgtcaccttc cagtcgcgtc
    32461 tcgccaatcc agatttcacc gctatccgga cgttcactgc cggtaagcat tcgaatgagg
    32521 gtcgatttgc ccgcgccgtt tttgcctaac agcgcacgaa cctcgccttt attgagcgtg
    32581 aagttaacgt tatccaacgc aacgacgccg ggataacgct tatttcctgc caccactttt
    32641 gctaccggga ctgcctctgt tgccgtgaac atagggacct ctgcgaatca gcgattaggg
    32701 cagaccatca acgtgcgttg ccagccactg tttaccgtct tccgttttgg tatagagatc
    32761 gataggcacc tgaatcactt tttcaccgtc ggcttgttta ttgataacct tcaatgtttg
    32821 cgcgaaaaca gcattgccca ttttcttacc ggaaatatcc actaccgctt tcagcacctg
    32881 attgttttcc agctcctgag caatttcggt tgtcatatcc gaaccgaaaa cagcaatttt
    32941 tccggcctga ttttgattac gtaccgcttt taccgcgccg agtgtcgcac cgcccgattc
    33001 ccccataatg gcgttgagat ccggcgtgga gataatcagt ttttcaccaa cggaaatcgc
    33061 tttatctaaa acagtccctt cctgattagc gacaatttgc gcgccgggaa cgcgggattt
    33121 taatacttct tcaaatcctt tacgtcgctg cacacaaact tcaaaggctt cgcaattgat
    33181 gacggcaatt ttcggctggt caattttatt ggcaataaaa taatcggcgg cagcgttacc
    33241 cagtttttta ccaaattcca gcgggtcacc caccagatac gccgagacat atttatcgac
    33301 ccccttttga ttaatacagg tgttgtagca aatcaccgga atacccgctt cactggcgcg
    33361 acgaacggta cggctactgc cattttcaga cactgccgat aaaataatag catcgacatt
    33421 acgcgccacg agggtatcaa caaaggtact ttctttcgaa atatcaccct gggcgttagt
    33481 ttcaattaac tgtacctgta ctgaagaatc ttttgccgca tcctgaactc cctgacgcac
    33541 tccagcgtaa tatccctgag tatcaaggta tattgcgcca atggtcattt ctttatccgc
    33601 agctctggca aatagtgcgc tacctaatag cgtagccatt aataatagat tacgggttgt
    33661 tctcattttt ttaggcatag agcctcctgt agggttttta ttaacaacgg cttattctaa
    33721 ttattttgtg atgagcggca gcgcgtgccg ctcaaatatt acagttaata gggtttagtg
    33781 aacgtagtta aaaataaaag gcatcacggt ggtgaataat gccgctttcg gccaggccgg
    33841 attaatcgcc gtcaattcag cgcgagcaga ttcgtattgc gcaatctcgc ctaatcccgc
    33901 ggccgccagc atcgccacca tcaggcattt ttctttatgc tgctgttgta aatcgccata
    33961 cagcgacaac aagtcaggct gcgagacggc aaagaaatcc gcttcgatac tggttttcgc
    34021 catctcttgc gcccactgtt tcatttcact aaacagttgc tgtgcggtgt gttgttcgcc
    34081 cagtaatcgc agcgccattc cttgccagaa gaggtaatca accggctgat cgttgtaata
    34141 gctgtggatg ttaatggtgc gatcgccggt cgccgccaga tgtaaacagc gcgtcgcttc
    34201 agtttcatcg ccctgggctt tggcgcatat cgcctgccag aaccagatgt cgttatcagt
    34261 ttgccccggt aaacggcctt cgcttaaatt ctccggataa tgcagcgcgg catgaagcag
    34321 ttcgctggcc tgctgcggct ctctggcatc aagatgctgc caggcgcgta ataactggtt
    34381 gaggataaac tgactggtga ccttcccttc cccgccttcc cacgggtgga atttgcgcgt
    34441 ggcgagaatg tccgccgctt tgtctgcctg ccccgtgaga tgccacaaat tgagcagttc
    34501 tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta ttttccagac gcgccagtcg
    34561 tttctccggt gtggttccac ttagcttatc aagcaaatcc cgttcgaaaa gcagacgtgc
    34621 atcctgcggc gcaagctgat aagcattatc aagataacgc gcggccagct cataatcgtg
    34681 ttgcttattc cacgcatgga tcgctaaccc gcgccagccg tcggcaaact ccggcgacat
    34741 ctctacgcaa cgttgccata aggcaatggc ttcgttgtag ctacgtttgt tgtagtagaa
    34801 gcaggccagt aaatggcgag caaaccagca ctcttcaata ctctccagcg ccgccacttc
    34861 ttccagcgta ttcgggaaac ggacaaactg cgggaagaca tcaatggctt ttgcgaccag
    34921 ttcgccacgt tcggctttcg gcagcaagct ggcttgcagg taaagcggca gcgtgcgctg
    34981 gcagtccagt gcattcagca tctctgccgc gagggtgggc attccccagt taatcagttg
    35041 cccggcggtc atcagggcgt taacgtcgcg tccctgacac agaccgcgcc actgcgcgag
    35101 agctgattcg ctacgaccat cgaaccagtt caggcaccac agagtggcgt tcagcggata
    35161 atcgcgcagc agtttctcgc gctgcaaacg cgcgttgtcc tgacgaccac ttaacaccag
    35221 cagcagatta tgcaggcaaa gcacttcctg attgattggg caggcgcgaa gactttgttg
    35281 gcaaaaatcc agacccgcgt cgaagttacc attacgcgcc gccagtcgtg ccagaccata
    35341 atagcctccg gctttactgt tgccgctcca gaccgcacgc cagaaatcct cttcggcttg
    35401 ttgatattgt ccctgacgtt cgtaagcact ggcgcgaatc aaactcgcct gtccgcactg
    35461 cggatttttg ttcagcgcat gtgcgcgttt cagagcctga gtggcatacg ccaccgcttg
    35521 cgggaaatct gcgcggttat actccagcat cgccagcgcc agattacagc ggtaatccag
    35581 cggatccagc gccacgccgc gcaggtagta atcgaacggt gaacggctgg cgtgatgata
    35641 ttgctccaga tgctgaccga taaaccaggc ttcatctgta ctggtaatgt cttgtgctga
    35701 cagtggcgct ttggcgacgt ccggcaacgg caactcttgc ggctgatgtt cctgataact
    35761 cagtacaata ttgccgtcgg catcagagag ctcaatggtc agtctttcag gattgatacc
    35821 gtgtaacacg ccctggatgg cggtcgctgg tgtcagggcc acggcatcat cgagcaacgc
    35881 gtcgcatttg ccgatttcgc ggatcgccag gcggtatccg ttcaacggag agatggcata
    35941 cagcccccac tcaatccccc gctcactacg ctggagtttg attaccgcat cgcgggaggc
    36001 gttttgcacc atgcccagag aatggtaagg caggaaaaac tgctcaaaac gcttctcttc
    36061 gtaagcatca agccgggtaa aatcaggctg gttatcggca aaaataccgg tcatcagttc
    36121 gatatacggg ccgttattat cagttaggct cttatcccac gcctggccaa attcactgtg
    36181 tccccagctc cactgctttt tacctggcgc aatatggtgg ttggcaacgt gtagcaaacc
    36241 gccatcttca tcgtgacacc acgcgccaac aaaatcgtac tgtgattttt cagccatata
    36301 tgaggttgga acgggcacat ttttatagcg agaaatgtcc actccagcgg agtagtccac
    36361 tttgtagtaa gtgccggtgg cgatagggaa agcggagacg gcccgtttgc cgtgatcaaa
    36421 caccgccgtt acatccggcg gaaagacgct ctgatgccct tcaccccctt tcactgccgg
    36481 gttggcccac cacaagaaat gacgcggcgt ggcgttgccg ttatagacgc ggctggcgat
    36541 ttccagcgcc gcccggtcag ggcgcagggt gaaacctgtc atcacctgta aaccatgcat
    36601 cggctccgtt tcgcctaccc acaccgtctg tgcgccgtct tcatgggctt cgagggtgaa
    36661 atcaacgggc ataaaggtgg tcgggcgatg gtgttgcggc cagttaaact caatcccgcc
    36721 ggagatccac ggtcccagca gccccaccag cgcaggttta atgacttcat tgtgatagac
    36781 aaaatcgcgc tgtttcactt tatcccatgc gcgatgcacc cgaccgccca attccggcag
    36841 gatcatcact ttgatgtagt cgttttccag ccacaccgcc tgccaggatt tcagggtttt
    36901 ctgctcgctc agcgtatcgg tcacgccgta gggataaacc gcgccggacg atccctgata
    36961 aacgcgattt tccaggaaca tgggatgtat atcctgcggc ccggtttcat aggtcgggat
    37021 ctcaacgcgc tcttgccaca cttttactgg agtcatggtg ccctcaatat taacaagaca
    37081 tactgaatta aaagatttgt ggcagtgtat tgaacaatct ggcaatgttt tcgcggaata
    37141 atcacgcaat taactaaaca aggtttagtg aagatgagag cctgcattaa caatcaacag
    37201 attcgccacc ataacaaatg cgtgattctg gaactgctgt accggcaaaa gcgcgccaat
    37261 aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg cagtcagtaa tattttgcag
    37321 gaactggaaa gcgaaaaacg ggtggtgaat atcgacgatg aaagccagac gcgcgggcat
    37381 agtagcggta catggctgat tgcgccggaa ggtgactgga cgctgtgtct gaacgtgacg
    37441 cccaccagta ttgagtgtca ggttgctaat gcttgtttaa gtccgaaagg tgaatttgag
    37501 tatttacaga ttgatgcacc gacgccgcag gcgctgctgt ccgaaatcga aaaatgctgg
    37561 catcgccacc gtaaattgtg gccggaccgc accatcaatc tggcgctggc aatccacggt
    37621 caggttgatc cagtgactgg agtgtcgcaa accatgccgc aagcgccgtg gacaacgccg
    37681 gttgaggtga agtatctgct ggaagagaag ctgggcattc gggtgatggt cgataatgac
    37741 tgcgtgatgc tggcgctggc ggaaaaatgg caaaataatt cgcaggaacg ggatttctgc
    37801 gtgatcaacg ttgattacgg cattggctcg tcgttcgtga ttaacgagca aatttatcgc
    37861 ggcagtttgt atggtagcgg acagattggt cacaccatcg ttaatccgga tggcgtcgtc
    37921 tgcgactgtg gacgttacgg ctgcctggaa accgtcgcct cgttaagtgc attaaaaaaa
    37981 caggcgcggg tatggctaaa atcacaaccg gttaatactc agcttgatcc tgaaaaactg
    38041 actacagcgc agttaatcgc tgcctggcaa agtggagaac cgtggatcac cagctgggtt
    38101 gatcgctctg ccaatgccat tggtttgagt ctgtataact tcctcaacat cctcaatatt
    38161 aatcagattt ggttgtacgg tcgcagttgt gcctttggtg agcactggct taatactatt
    38221 attcgccaga caggatttaa cccgttcgac cgcgacgaag gaccgagcgt gaaagcgacg
    38281 caaattggct tcgggcaatt aagccgcgca caacaggtgc tgggaattgg ctatttgtat
    38341 gttgagacgc agttacgaca gatttgatgg cgcgataacg tagaaaggct tcccgaagga
    38401 agcctttttc aacagattaa ccgtttcgct tatgcgtaaa ccgggtaacg tgcgcagatg
    38461 tcgagaactt tacctttgat gcgctcgata acggcttcat cattgatgct gtccagcacg
    38521 tcacacatcc agccagccag ttctttcgct tctacttctt tgaagccgcg acgcgtaatt
    38581 gccggagtac ccacgcgaat accggaggtc acaaacgggc tcttcggatc gttcggtacg
    38641 ctgtttttgt tgacggtgat gttagcacgg cccagagcgg cgtctgcttc tttaccggtc
    38701 aggtttttat cgaccagatc aaccaggaac agatggttat cagtaccgcc ggaaaccact
    38761 ttgtagccgc gttccaggaa cacttctacc atcgctttag cgtttttagc gacctgctgc
    38821 tggtaagttt tgaactcagg ctccatcgct tctttcagag caaccgcttt accggcgatt
    38881 acgtgcatca acggaccgcc ctgaccacca gggaaaacgg cagagttcag ttttttgtac
    38941 agctcttcgc taccaccttt cgccaggatc agaccgccgc gcggacccgc cagggttttg
    39001 tgagtggtgg tagtaacaac gtgagcatga ggaaccgggt tcgggtagac gccagcagca
    39061 accaggcccg caacgtgcgc catatcaacg aacaggtaag caccgatgct gtcagcgatt
    39121 tcacgcattt tcgcccagtc caccacgccg gaatatgcag agaagccacc gataatcatt
    39181 ttcggtttgt gttctttggc ttgtttttcc agatcggcgt agtcgatatg accggtagca
    39241 tcaataccgt aaggaacaat gttgtacagt ttaccggaga agttaaccgg agaaccgtga
    39301 gtcaggtgac cgccatgcgc caggttcata cccagaacgg tatcacctgg ttccagcagc
    39361 gcggtgtaga ccgcaaagtt agcctgggag ccggagtgcg gctggacgtt agcgtagtca
    39421 gcgccgaaca gttctttcgc acgatcgatc gccagttgtt caacgatatc aacatactcg
    39481 caaccaccgt agtagcgttt gcccggataa ccttcagcat atttgttggt cagctgagaa
    39541 ccctgcgcct gcattacgcg cgggctggtg tagttttcgg aggcgatcag ttcgatgtgc
    39601 tcttcctgac gtactttttc ctgctccata gcctgccaca gttcggcatc ataatcggca
    39661 atgttcattt cacgctttaa catccgcatc tcctgactca gctaacaata aaatttttgg
    39721 cctttatagg cggtcctgtt ggacaacggc gaacagtata accgaatcat tgtgcgataa
    39781 caggtcttga caaaggaatt tacgcaaacg attaccttca ggctacgcaa ggctttggag
    39841 aataaagagc ttgcaaccgg aaacggattt cttttcaggt ttgtgatgca aatttttcac
    39901 ttcatcacat tctttctgaa aaacaccaaa gaaccattta cattgcaggg ctatttttta
    39961 taagatgcat ttgagataca tcaattaaga tgcaaaaaaa ggaagaccat atgcttgacg
    40021 ctcaaaccat cgctacagta aaagccacca tccctttact ggtggaaacg gggccaaagt
    40081 taaccgccca tttctacgac cgtatgttta ctcataaccc agaactcaaa gaaattttta
    40141 acatgagtaa ccagcgtaat ggcgatcaac gtgaagccct gtttaacgct attgccgcct
    40201 acgccagtaa tattgaaaac ctgcctgcgc tgctgccagc ggtagaaaaa atcgcgcaga
    40261 agcacaccag tttccagatc aaaccggaac agtacaacat cgtcggtgaa cacctgttgg
    40321 caacgctgga cgaaatgttc agcccggggc aggaagtgct ggacgcgtgg ggtaaagcct
    40381 atggtgtact ggctaatgta tttatcaatc gcgaggcgga aatctataac gaaaacgcca
    40441 gcaaagccgg tggctgggaa ggtactcgcg atttccgcat tgtggctaaa acacctcgca
    40501 gcgcgcttat caccagcttc gaactggagc cggtcgacgg tggcgcagtg gcagaatacc
    40561 gtccggggca atatctcggc gtctggctga agccggaagg tttcccgcat caggaaattc
    40621 gtcagtactc tttgactcgc aaaccggatg gcaaaggcta tcgtattgcg gtgaaacgcg
    40681 aagagggtgg gcaggtatcc aactggttgc acaatcacgc caatgttggc gatgtcgtga
    40741 aactggtcgc tccggcaggt gatttcttta tggctgtcgc agatgacaca ccagtgacgt
    40801 taatctctgc cggtgttggt caaacgccaa tgctggcaat gctcgacacg ctggcaaaag
    40861 caggccacac agcacaagtg aactggttcc atgcggcaga aaatggcgat gttcacgcct
    40921 ttgccgatga agttaaggaa ctggggcagt cactgccgcg ctttaccgcg cacacctggt
    40981 atcgtcagcc gagcgaagcc gatcgcgcta aaggtcagtt tgatagcgaa ggtctgatgg
    41041 atttgagcaa actggaaggt gcgttcagcg atccgacaat gcagttctat ctctgcggcc
    41101 cggttggctt catgcagttt gccgcgaaac agttagtgga tctgggcgtg aagcaggaaa
    41161 acattcatta cgaatgcttt ggcccgcata aggtgctgtg atttgatgtt gccggatgga
    41221 aacatccggc aacccttgac gcggtttaaa ttgccgcgtc gtcctcttca ccggtacgga
    41281 tgcgaatgac ccgtgccacg tcaaagacga agattttacc gtcaccgatt ttgccggttt
    41341 gcgccgtgcg aataatggta tcgacacagg tatcgacaat gtcgtccggt acgacaatct
    41401 caattttcac tttcggcaga aaatccacca tatactccgc gccgcggtac agctcggtat
    41461 ggcctttctg gcggccaaaa cctttcacct cagtgaccgt catgccggta atgccgactt
    41521 cagccagtgc ttcgcggaca tcgtccagct tgaagggttt aataatcgca tcaatctttt
    41581 tcatgctatt ccttgaaaag gtcgcctgtc ttttgatctg ctaaacgtaa cacataacgc
    41641 caattcattc cttgaaatcg tttgcatcca gctcgtgtcg ggaaagcagt ttataaaatt
    41701 ctgtccggtt gcgccccgcc attctcgccg cgtgggtgac gttgcctttg gtaatttgca
    41761 gcagcttacg caaatagttg agttcaaact gattacgtgc ctcaacaaag gttggcagcg
    41821 ccgtattttc accctccagc gcctgctcta ctaatgcatc gctaatcacc ggagatgagg
    41881 tcagcgccac gcactgttca atcacgttga ccaactggcg cacattaccc ggccagctcg
    41941 cggtcatcag gcgtttcatc gcatcggtgg aaaacgcgcg gacaaacggt ttatgtcgct
    42001 ctgccgcctg gcgcaacagg tgatttgcca gtagcggaat gtcttctgtg cgctccgcca
    42061 gtgccggaat tttcaggctg acaacgttga ggcggtaata gagatcttcg cggaattccc
    42121 cgcgcgccat cgcttttggc agatcacggt gagtggcaga aataatccgc acattgatat
    42181 caatatcgcg gttactgccc agcgggcgca ctttacgctc ctgcaacacg cgcaacagtt
    42241 tgacctgcaa cggcgcaggc atatcgccaa tctcatcgag aaatagcgtt ccgccttccg
    42301 ccgcctggaa taaaccttcg cgattgctga cagcgccagt aaacgcgcca cgcgcatgac
    42361 caaacagctc cgactccagc aattgctcag gcaatgcacc acagttaata gcaataaatg
    42421 gtttgctatt gcgcgggctg gcgttgtgga tagcctgggc gaaaatctct ttcccggtgc
    42481 cgctctggcc attaatcaaa acgctgacgt ctgattgcgc caccagccgc gcctgttcca
    42541 gcaaacgcag catcagcggg ctgcgggtga caatcgcctc gcgccagcgt tcgtcggtgg
    42601 ctggcgcgga ttgttccagc gcatcatcaa ttgcctgata tagcgcgtct ttatcaacag
    42661 gcttggtgag gaaactaaaa acgccctgct gtgttgcagc aacggcatcg ggaatagaac
    42721 catgcgcggt aagaataatt accggcattc ccggctgcac tttctggatt tcagcaaaca
    42781 gctgcatacc gtccatttca tccatccgca ggtcgctgat gactaaatct actttttcgc
    42841 gattcagtac ccgtaatcct tcagcgccac tttccgccgt gaccacgctg tagccttcgc
    42901 tggtcaggcg caggccaagc agtttcagca atcccggatc gtcatcgacc aataataaat
    42961 gcgcaggttt atggctcatc aggagtgacc tcatgggtgg atggcgcggg cttttcactc
    43021 tcgtgtggcg tatccggcga gaaatttcca gccggtttgc gggtcgagag ctggcgttca
    43081 atatcagtca ggttttccag cttgcgggtg gtgagttcca gttgctgctg tagaacgtga
    43141 tgttgctggc gcaatgtatc cagctcgctg tcgctggact gctggagttt actgtagcgt
    43201 tggcgctctt ccgccagttg tagttgcagc gcctgaccgt cgcgccagag ttgatacagt
    43261 gggcgaacct gtgccgggat ctcggtactt aacgcttcaa tacgcgcgac cagctggcgg
    43321 cgctcatatg gcgtaatttt ggcgtcggcg agcaaaatcc cttgtttaaa ggtattttgc
    43381 cagctgccgt cgtcatattg gcgagcttgc tgacgcgact gcgcaggcat taaacgatca
    43441 gcacaatcca tcgcccgcag ccagtaaagc ggattggttt cggttgattt accttgcagc
    43501 gcccagatgt cgctacattc agtagaaaga tagtcagcca gttgataaac cggaattttt
    43561 tcttctgctg gcgtatcaat ggctggctta ttgtgattct gcacgcaacc tagcaatgcc
    43621 agacatggca gccctgccag ccacagccgt cggggcaata atcgttgaaa aatgtgtcgc
    43681 atattcacca gacttaaagc ctatcccagt gggcgtaatt gttgcagaca gtttggacat
    43741 ggacagcgcg gagaaaccgg agcgtacatg tcgtacgtga ggatttcgag cactgcccgg
    43801 ggccgaactg acaaataaaa tagcctgatg ggataggctg ttagcattat ttcgtgtttt
    43861 tcgacgacgg taattcaatg cggaaacaaa cgtcttgccc gctctcgtcg accagataca
    43921 gttccccttg catacggcga atacaatccc tggcaatgct taatcccaga ccgctgccct
    43981 tcaccgcccc ttttcgctgg tggcttccct gaaaaaaggg ttcgaagatc atggcgcgtt
    44041 cctcttgcgg aatgggcgtg cctgtattga tgacatcaat ataaacccgc gcaccatgta
    44101 aactgctgcg aaggcaaatg ttaccggatt cagccccgta gtgcaccgca ttggagtaaa
    44161 gattatccag tacgctcatc agcagcattg gctccgccag gcaagctgtt gctttgagat
    44221 cgacgtcggt atgcatcatt ttagcccgtg cgggcaggct atgagcagaa actactgtct
    44281 ccaccagcgg tgctaactca acattatcta gttccaccgc actgtccgcc tgtttacggt
    44341 tgtaatcaag cagttgttcg atcagttttt gcaaattgcg gctgctgcta tcaagaatgc
    44401 tcaccacctc tttttgctct ggcgtaagcg gcccgacaac ctgatcagcc agtaattcag
    44461 tgccctcgcg catactcgcc agtggcgttt ttaattcatg agataaatgt cttaaaaatt
    44521 gatggcgttg ggattccagc catgacaggc gctcacttaa ccaaagaata cgttgcccaa
    44581 ccgagcgtaa ctcgctcggt ccactgaacg aaacgctatt gcccagagaa cgcccttccc
    44641 ccaaccgatt gatcatccgt tcaatatttt tcaccggccc gataatcatc cgcgtgaaaa
    44701 gcagtaccat taccagactc accagaaata gcaccagcga ttgccaacca aaatattgcc
    44761 cacgttcggc gatttcacgc tgaagttgct gcccacgaga gaacactact gtgcgcgtgg
    44821 cctgtaccat ttcggtattg gcactggcaa aggcttccag acgcgcggca gcagcagcat
    44881 cgggaccact gttgttgcac tgaagttggg ccagattgtt caagtcctga cgcaatgcct
    44941 ggtaaagttt atcgtctggc agtacgcctg cgtgggcatc gagcatttcg ctgtaacgct
    45001 tgcgctggct ttgataaacc ttcgcaagcg tagggtcatc cagcacgcaa tactgacggt
    45061 agctacgctc catctccagc gccgcgttgg tcattgcttc gctgcgccgg gcatcgataa
    45121 gcgtggtgcg gttgaccagc gccgcctggt cgctaagcgc attcaggctt tgccaggctt
    45181 gccatgccag caccaacagg ggcagcagaa tcagcaaaaa tgccagcatt accagttgtc
    45241 gtaatgagcg gggaaaaacg ggccagcgtt tcaaggtgtt actctcgtca gacgcgaata
    45301 gcctgatgct aaccgagggg aagttcagat acaacaaagc cgggaattac ccggctttgt
    45361 tatggaataa ggcggtgcct aactcgacgt ttcgcccgat ggttgatata gctacgctga
    45421 tatcagaagt tggacggcag gcaccttgtt gtgcgtcatt cgaattttat gtagcacgtc
    45481 ccgaaggggc tgacataagt cggtgaatga gccactgatt actattatgc agtaactgtg
    45541 ccaataaaga aaatagtttg gtaacgtact gattatacgt tgctttgagg ggtttatggc
    45601 ttccccgcta tttgaatgta aatcagccac tgtgtcgcta aaaagagaca acttaagaaa
    45661 aacttattag attacaataa aatcaatcat ttaagtgtcg ccagaaagcg acacggtaaa
    45721 ccacccattg tcgtgattta cagacacaaa aaagctcccg aagttgggag cttataatag
    45781 cggttgtatt taatgccgga tgcggcgtga atgcctcatc cgggctgcaa aaccaatgag
    45841 tttgacggct taccccaact gcttacgtgc attgcggaaa atgcgcatcc atgggccatc
    45901 ctcgccccag ttttcaggat gccaggagtt gctgacagta cggaaaacac gttccgggtg
    45961 cggcatcata atggtgactc gaccgctttc agtcgtgacc gccgtaatac cgttcggaga
    46021 accgttcggg ttagccgggt aggtttcagt gactttgccg aagttatcga catagcgcag
    46081 tgccaccagc cctttgcttt ccagtgccgc cagatgcgcc gcatcacgca cttccacgcg
    46141 cccttcacca tgagagacag caatcggcat ttgcgagccc accatcccct gcaacagcag
    46201 cgacgggctt tgggtcactt caaccaagct gaaacgcgct tcaaagcgat cggaggtatt
    46261 gcgcacaaaa cgtggccaca actcactacc tgggatcagt tcacgcagat tagacatcat
    46321 ctggcaaccg ttacataccc ccagcgccag cgtttgcgga cggtggaaga aggttgcaaa
    46381 ctcatcgcgt acacggtcat tgaacaggat agacttcgcc caaccttcac cggcacccag
    46441 cacatcaccg taggagaaac caccgcacgc gaccagggcg tggaaatcct ccaggcccgt
    46501 gcgtccagcc agcaggtcgc tcatatgcac gtcgatagca tcgaagcctg cacggtggaa
    46561 agctgccgcc atttcaacat gcgagttcac gccctgctca cgcagcacag caactttcgg
    46621 acgtgcgcca gtggcaatat acggtgctgc cacatcttcg ttgatatcaa acgacagttt
    46681 cacattcagg cccggatcgg cgtcgtttga tttcgcctga tgctcctgat cggcacactc
    46741 cgggttgtca cgcaggcgct gcatctgcca ggtagtttct gcccaccaga cacgcaacgt
    46801 agtgcggctt tcgctgaata cagtctgccc gttggcggta atcacaaaac ggtcaccgga
    46861 aaccgcctgc ccaacataat ggacgcaatc agcaagccca tgctgtgcca gtacggactc
    46921 gaccgcttca cggtcagcgg cacgaacctg aatgaccgca cccagttctt cgttaaacaa
    46981 cgccgccagg cgatcgtcac ccagagtggc gatatccgcg tcaatgccgc aatgaccagc
    47041 aaaggccatt tccgccagcg ttaccagcag accgccatca gagcggtcgt gatacgccag
    47101 caacttacgc tgtgcaacca gcgcctgaat cgcgtcatag aagcctttca gttgcgcgac
    47161 atcgcgtaca tctgccggtt tgtcgccaag ctgacgataa acctgcgcca gcgccgttgc
    47221 gcccagcgcg ttattgcctt tgcccaaatc aatcagcagc agtgcgttat cttcggtaga
    47281 aagctgcggc gtgatggtgt gacgtacatc ttccacgcgg gcaaatgcag aaatcaccag
    47341 cgacagcggc gacgtcattt cgcgctcttc gttaccttcc tgccagcggg ttttcatcga
    47401 catagagtct ttacccaccg ggatcgtcag gcccagcgcc ggacaaagct cttcgcccac
    47461 ggctttaacg gcttcataca ggcccgcatc ttcaccaggg tggcctgccg ccgccatcca
    47521 gttggcggaa agtttaatgc gtttgatatc gccaatctgt gttgcggcaa tattggttaa
    47581 cgcttcaccg accgccagac gggcagaggc ggcgaaatcc agcagcgcaa ccggcgcacg
    47641 ctcgccaatc gccatcgctt caccgtagta gctgtcgagg ctggcggtag tgaccgcgca
    47701 gttagcgacc ggcacctgcc acggccccac catctgatcg cgcgccacca tgccggttac
    47761 gctgcggtca ccgatggtca ccaggaaagt tttctccgcc acggtcggca gatgcagtac
    47821 gcgtttcacc gcgtctgcaa tggtgatccc ttcacggacc agcgcatcgc ccttcgcttt
    47881 cagcgtttgt acatcgcggg tcatcttcgg cgttttacca agcaggacgt ccagcggcag
    47941 atcgatcggc tgattatcaa aatgacgatc gtgcagagaa agatgcagtt cttcggtcgc
    48001 ttcaccaatc accgcgtagg gtgcgcgctc acgcttacac agttcgtcaa acagcggtaa
    48061 ttgatcggca gcaaccgcca gcacgtagcg ttcctgggat tcgttacacc agatttccag
    48121 cgggctcatg cccggttcgt cgcttagaat atcgcgcagt tcaaatttac cgccgcgccc
    48181 gccgtcgctc accagttccg gcatcgcgtt agaaagaccg ccagcgccga cgtcgtggat
    48241 aaacaggatt gggttggcat caccaagctg ccagcaacgg tcgatcactt cctggcagcg
    48301 acgctccatc tccgggttgt cgcgctgtac ggaagcaaag tcgaggtcgg catcagactg
    48361 acctgacgcc atggaagacg ccgctccgcc gccaagaccg atgttcattg ccgggccgcc
    48421 gagaacgacc agtttcgcac cgacgttgat ctcgcctttt tgtacgtgat cggcgcgaat
    48481 gttgccgatc ccgcccgcca gcatgatcgg tttgtgataa ccgcgcagct cttcgccgtt
    48541 gtggctgttc actttttctt cataagtacg gaagtagcca ttcagtgccg gacgaccaaa
    48601 ttcgttgtta aacgccgcgc cgcccagcgg gccttcggtc atgatgtcca gcgcggtgac
    48661 aatgcgctca ggcttaccga aatcttcttc ccacggctgt tcgaagccag gaattcgcag
    48721 gttggaaacg gagaaaccaa ccaaacccgc tttcggcttc gcgccgcgcc cggtggcacc
    48781 ttcatcgcgg atttcaccgc cggaaccggt cgccgcgccc ggccacggag aaatcgccgt
    48841 cgggtggttg tgagtttcga ctttcatcag aatatgcgcc ggttcctgat ggaaatcgta
    48901 gcggcccgtt tcgtggtcag caaagtagcg gcccacttca gaaccttcca ttacggcggc
    48961 gttatcttta taagcagaga gaacgtgatc tggcgtggtt tcgaaagtat ttttgatcat
    49021 cttgaacagc gatttcggct gctgttcacc gtcgataatc cagtcggcat taaaaatttt
    49081 gtggcggcag tgctcagagt tcgcctgagc gaacatatac agctcgatgt cgttcgggtt
    49141 acgaccaagc tttgtgaaag catcctgcag atagtcaatt tcatcttccg ccagagccag
    49201 gccaagacgc aggttagcgt cgatcagcgc ctgacggccc tgccccagca aatcaacgct
    49261 ggtaaccgga gtcggttgat ggtgggcaaa caactgctct gcatcatcta aagcaaaaaa
    49321 gaccgtttcc atcatgcggt cgtgcagttc agcggtaacc tgctgccatt gttcattggt
    49381 cagcgtaccg gcttctatat agtaagcaac gccgcgctca aggcggttta cctgttgtag
    49441 cccgcagtta tgagcaatat cggtcgcttt cgaagaccag ggagagatgg tgccaggacg
    49501 cggggtcacc agcaggagtt tgccttgcgg ggcgtggctg gcgagtgccg ggccatattt
    49561 cagcaggcgt tcaagttgtg cgtgctcatc atcgtttaac ggcgcattga ggtcagcaaa
    49621 atggacatac tcggcgtaaa tattgtgaac cgggagcctg gcagcctgaa aacgtgccag
    49681 cagtttgttg attcggaatg ccgacagtgc aggcgaacca cgcagaattt ccatcataag
    49741 tctctcgtct tctaagcttt cggtgtaccc aaggggggaa acgggcgtca ttataaagaa
    49801 tctgatgcgc tgacgaaacc gtttgcgtgg aaataaaatc accatcgtga attagcaacg
    49861 cgtgccgcca atggctgtaa taagttgcca tctggcgcag gtttacgcaa aatgccgctc
    49921 atttatgagt aaacctttca ctattattac attttttcaa gctgggacgc gcacgacaca
    49981 gagaattaac taattgaaaa aattaaagat taattatctg ttcatcggca ttctggcact
    50041 gctgctcgcg gtcgctctct ggccatccat tccctggttt ggtaaagccg acaaccgtat
    50101 cgccgccatt caagcgcggg gagagttgcg tgtgagcacc attcatactc ccctgacgta
    50161 taacgaaatc aacgggaaac cttttggcct ggattacgaa ctggcgaaac agtttgccga
    50221 ttacctcggc gtaaaactga aagtgaccgt gcggcagaat atcagccagc tgtttgacga
    50281 cctcgataat ggtaacgccg acctgctggc ggcaggactt gtctataaca gtgagcgggt
    50341 aaaaaattat cagcctggcc ctacctatta ttccgtgtca caacaactgg tttataaagt
    50401 gggtcagtat cgcccacgta cgctgggcaa cctgacggcg gagcaactca ccgttgcacc
    50461 gggtcatgtg gtggttaacg atctccagac cctgaaagaa acaaaattcc cggaattaag
    50521 ctggaaggta gacgacaaaa aaggctctgc ggaattaatg gaagatgtca tcgaaggaaa
    50581 actcgattac accattgctg attctgtcgc catcagcctg tttcagcgcg ttcacccgga
    50641 gctcgccgta gcgctcgata tcaccgatga acaaccggtg acctggttta gcccgttaga
    50701 tggcgataat accctttccg ccgccctgct cgacttcttc aacgaaatga atgaagacgg
    50761 tacgctggca cgcattgaag agaaatacct ggggcatggc gatgattttg attacgtcga
    50821 tacgcgcaca tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc ccctgtttga
    50881 gaaatacgcc gaagaaattg actggcgttt gctggccgct attgcttatc aggaatcgca
    50941 ctgggatgca caggccactt caccgacggg tgtgcgcggc atgatgatgt taaccaaaaa
    51001 taccgcgcaa agcctcggca ttacggatcg taccgatgcc gaacagagca tcagcggtgg
    51061 cgtgcgttat ttgcaggata tgatgagtaa agtgccggaa agtgtgccgg agaacgagcg
    51121 gatctggttt gccctcgctg cgtacaatat gggctatgcg catatgcagg atgcccgcgc
    51181 cctgacggca aaaaccaaag ggaatcctga cagttgggct gacgtaaaac agcgtctgcc
    51241 tttacttagc cagaaaccct attacagcaa gctgacttac ggctacgctc gtgggcatga
    51301 agcctacgct tatgtcgaaa atattcgtaa gtatcagatt agcctggtgg gttatctgca
    51361 agagaaagag aagcaggcta cagaagcggc gatgcaactg gcgcaggatt atccggcggt
    51421 atcgcctacg gagttgggca aagagaaatt tccttttctc tcgtttcttt cccagtcgtc
    51481 atcaaactat ttgacccatt ctccctctct gctgttttcc aggaaaggga gtgaagagaa
    51541 acaaaattaa tccgtcgagg attgcgcttt tttctgcgct ttaatttcct ggcggcgcat
    51601 gcgaaagaag tcactgagca acgccgcgca ctcatccgcc agtattcctt ccgtaatttc
    51661 cactcggtga ttcatacccg gatgatgcag cacatccatt aaagatcccg cagcgccagt
    51721 tttcgcgtca cgcgcaccaa agaccacgcg accaatgcga ctgtggatca tcgctccggc
    51781 acacattaca catggttcaa gcgtgacata caacgtggcg tcgatcagac gataattttg
    51841 catcaccaga ccgccctgcc tcagggccat gatttctgca tgtgcggtgg gatcatgtcg
    51901 accaatcgga cggttccagc cttcgccaat aacacgattg ttatgcacta ataccgcgcc
    51961 gaccggtact tcccgctcat cccaggcacg cttcgccagc gtcattgcat gacgcatcca
    52021 gtattcgtgg ctaaattcga cttcagacaa aaagaaaact ccggttataa aagcgcggcg
    52081 cattatacac ggacgctatg ctttactcta ttccagttgc tggagttcac cgcgcggggt
    52141 tactcgccag cgatgctggc aaaagtaaag cagcgggttg tcctgattac tgtcgctata
    52201 gccactgtac agccgcagcg gcgtgccgat tttgcgctcc agttgcgcga ccttttcatg
    52261 cccgaggcaa cgcatcgtca atacccaacc accatagcca cgctgaattt ggctggcgat
    52321 aagattcacc cgcggcagcc agggtgtatc gaaatagacc gcttcaacca gcggctgtgg
    52381 agaaccggta atcaaccaga tatcagcatc ggaacttaac aggtaggtgg ttaatcgctc
    52441 ctgaaccagc ggaaaggcgg taacattgtc acgaaaccag cgtacgaaat cggcctgcaa
    52501 cgcctgtaaa cgtgcttcgc tgtgaccaaa ggtgcatccc cacagaagca gactcatcgg
    52561 ccagcgtgcc gcacgacctt ttatcaataa cgcaatggct ataatcggca acaacggcag
    52621 gacaagtaac gcattcagcg gttggcgacg cagtaaatag cgcagaaaac tgccgaacat
    52681 atcctgctga tgcaatgttc catctaagtc aaaaaacacc acacgacgct cgtgagttgc
    52741 caaaccatac tcctctggac tgaaacatcc tgattaattt tcgctactac atagcctaac
    52801 agatagatca tcacttttcc ggcaacaatc gaccgcatca tggctaactg gaatttttaa
    52861 ttcatggcaa ttagcggcaa tggaatataa aattcactcg cgtgtgtctc atattgcctg
    52921 ttgtcgccac tatttcatcg cgaggaaaga tatgaacggc ttacttcgta tccgtcagcg
    52981 ttaccagggg cttgcccaga gtgataaaaa actggcggat tatctgctgc tacaacctga
    53041 tacggcgcgc catttaagct ctcagcaact ggccaacgaa gccggagtca gtcagtccag
    53101 cgtcgtgaag ttcgcgcaaa aactcggcta taaaggtttt ccggcgctta agttggcgtt
    53161 gagtgaagcg ctggcaagcc agccggaatc gccctcagtg cccattcata accaaatccg
    53221 cggtgatgat ccgttacggc tggtcggcga aaaactgatt aaagaaaata ctgccgctat
    53281 gtacgcaacg ctaaacgtta atagtgaaga gaaactgcat gaatgcgtaa caatgttgcg
    53341 ctctgcgcgg cggataattc tgaccggtat tggcgcttcg ggtctggtgg cgcaaaactt
    53401 tgcctggaag ctgatgaaga ttggcttcaa tgctgccgca gtgcgcgata tgcatgcgct
    53461 actcgctacc gtacaggcgt cgtcccctga cgatctgtta ttagccattt cttacaccgg
    53521 tgtacgacgc gagttaaacc tggcggcaga tgagatgctg cgagtgggcg gaaaagtgct
    53581 ggcgattacc ggctttactc ccaatgccct gcaacagcgt gcttctcatt gcctgtatac
    53641 cattgccgaa gaacaagcga caaacagtgc ttcaatctct gcttgtcacg cgcagggaat
    53701 gttaacggat ttgctgttca ttgcgctgat tcagcaggat ctggaactgg caccagaacg
    53761 tattcgtcat agtgaagcgc tggtgaaaaa actggtctga gtaaagaatg cgcgtataat
    53821 gcccgcccgg tttgtgttgt tttgagagtt tccttatggc gctgttaatc actaaaaaat
    53881 gcatcaattg tgatatgtgt gaacccgaat gcccgaatga ggcgatttca atgggagatc
    53941 atatctatga gattaacagc gataagtgta ccgaatgcgt agggcactac gagacaccaa
    54001 cctgccagaa ggtgtgcccg atccccaata ctattgtgaa agatccgcaa catgtcgaga
    54061 cagaagaaca gttgtgggat aaatttgtgc tgatgcacca cgcggataaa atttaatatt
    54121 ctactctgga agtagagtat taattatttt actgggaagc cttaacgcca ttatatttat
    54181 ttaattgatg acattagcat aataattcac taagttaatt tatatagtat ctgcccagac
    54241 acttatttat agttattaaa ggcgcgtccg attggttcac cggacgcacc ttaagtacgt
    54301 ttccttgtgt tataagaaca gaaggatcag ctgtaaaaca gcaatgatga ttttgatgac
    54361 ccgtttaatc aggtatcggc aatcagtcat tcgtttttcc ttaaacaagg aatgcagccg
    54421 ttcagattag cccttacatc tccccaaaac tgaacgtgcg agttattgga atcgagtgct
    54481 gcactccaca ccagagcttt gacgacacca ctcgtttcaa tgggggaatt cagtggcatg
    54541 gtgtaaagca cagcaaaatt gccaataacg aaaccgattt taatgtactt atcaaaaagc
    54601 ggtcacgtta atcttttcat caatgtcgat ttcttcgtaa aggctcgcgt tttttatgtc
    54661 agagattgca agttgcttgt acaggtcaac gaaattgatc cataatcatt ttcgttggaa
    54721 tcgtgtgctg cacaaaatta aagttaaaaa gtaaaacccc cgttccttac cagttcgggg
    54781 gttttacttt ataaagagaa cggtattatt tttaactttc aataattacc gtggcacaag
    54841 catagtggcg ctcatcggcc agcgtcacat gcatatttgc aacgcccagc ttttccgcca
    54901 gttttaatgc ctcgccccac agtcgcagcc gtggcttacc aagctcatcg ttaaatactt
    54961 caaactgatt aaacgccaga ccattgcgga tcccggtgcc aaacgctttt gctgcggctt
    55021 cttttacggc aaagcgtttc gccagaaaac gcaccggctg gtggtgcgtt ttccaaattt
    55081 cccattcgtt atcgcttaat acgcggcgtg ccaggcgctc accggatcgg gcgatcactg
    55141 cttctatgcg agcgatctct acgatatccg tgcctaaacc taatattgcc attagccacg
    55201 cgcttccagc atcaggcgct tcatttctgc caccgcatct ttcagtccgg tcatcactgc
    55261 acgaccaata atggcatgac cgatattcag ttcgtgcatc tcagggatgg cggcaatggc
    55321 ttttacgttg tgataggtca gaccgtgtcc ggcgttaact ttcaaaccga gactagcggc
    55381 aaaggtcgcg gctttggcga tacgcgccag ctcttgcgcc tgttcggcgt cagttttggc
    55441 atcagcatag caaccggtgt ggatctcgat aaacggtgcg ccaacctctg ccgcagcttt
    55501 gatctgctct tcatcggcgt cgataaacag agaaacctga atcccggcat ccgccagacg
    55561 tttgcaggca tcgcgcattt tgtcacgctg ccctgcaacg tccaggccgc cttcggttgt
    55621 tacttcctga cgcttttctg gtaccaggca gcaaaaatgc ggcttcgtct caaccgcgat
    55681 cgccagcatc tcttcggtca ccgccatctc cagattcatg cgggtatcca gcgtctgacg
    55741 caggatgcgc acatcgcggt cagtaatgtg gcggcgatct tcacgtaaat gcacggtaat
    55801 gccgtccgcc cccgcctgct cggcaataaa cgcggcctgc accggatccg ggtaagcggt
    55861 accgcgcgcg ttgcgcagcg tagcgatatg gtcaatgttg acgcctaaca gtaattcagc
    55921 catgacaatc ctcatcattc ataatgtgtt ttcaccgttc gcttaggcat aaattgccgg
    55981 aacagttccc tgctctttaa aggtttaccg ccaagatacg gcttaagcgc catgcgggta
    56041 aagcgtttcg cggcgcgcag tgtgtctgcg tcaggaaatt cccgtgcgtt taacgctttt
    56101 aactgccttc cggtgaacgt tttattgtcg ataacgacgc ttgcgataaa ccctttttct
    56161 tcgcgataac gatacgtcat ggtgtcatct accggctcgc cgctacccgc acaatgggta
    56221 aaattgacgc cataacccag atgcccgagc agtgccagtt caaagcggcg cagcgcgggt
    56281 tctggcgtac cagtgacccc tgcaagagac tgaatgcagt gcaagtaatc gaaaaagagt
    56341 tcagagaagc gcgtctcgta ttccagtacg cgggagagaa gttcgttgat gtacagaccg
    56401 ctgtaaagcg tgataccgct taatggcagc gccagcgaga cagcttcagc actgcgcagc
    56461 gttttgactt cgccacgccc gccaaaacgt agcaagagag gggtgaaagg ctgtaatgca
    56521 cctttcaggg tagagcgttt agagcgtgcg cctttggcaa ccagacgcac gcgacccgat
    56581 tcctccgtga agacgtccag catcaggctg gtttcgctcc acgggcgact atgcaggaca
    56641 aatgcgcgct gccagccttc catcggagtt actcttaaag atcgtcaacg taaccgagac
    56701 tgcgcagtgc gcgttcgtcg tcggcccaac cggatttcac ttttacccac agctcaaggt
    56761 gaacaggcgc ttcaaacatt tcctgcatgt ctttacgcgc ttcaatcccg atggttttga
    56821 tcttggcccc tttgttgcca atgaccatct tcttctgccc ttcacgctca acgagaatca
    56881 aaccgttgat gtcataacca ccgcgttcgt tagagacgaa acgttcgatc tccacggtca
    56941 cggagtacgg cagttcagcg ccgaggaaac gcatcagttt ttcgcggatg atttcagacg
    57001 ccataaaacg ctgtgagcga tcggtgatgt aatcttccgg gaagtgatga gtcgcttcag
    57061 gtagatgctt acgcacgatt gccgcaatag tggcaacatt cagcccggtt tcggcagaga
    57121 ttggcacgat atcgaggaag ttcatctggc ttgccaggaa ctgcaggtgc ggcagcagat
    57181 cggctttctc ctgcacgttg tccactttgt tcaccgcgag gattaccggc gctttgcctt
    57241 cgcgcagttt gttgagcacc atttcgtcgt ccggcgtcca gcgggtgcct tcaacgacaa
    57301 aaatcaccag ctcaacatcg ccaatagagc tgctcgccgc tttgttcatc aggcggttaa
    57361 tagcgcgttt ttcttccata tgcaggcccg gtgtatcgac gtagatcgcc tgatacgcgc
    57421 cttcagtatg gatccccaca atgcggtgac gagttgtctg cgccttgcgg gaggtgatgg
    57481 agattttctg ccccagcagt ttgttcaaca atgtggattt gccaacattc ggacgtccga
    57541 cgatggcaat aaatccgcag taacttttat cgatgctcat tccagctcca gttttttcaa
    57601 cgcctgttcg gcggcagcct gctcagcctt acgacggctt gaacctgtgc caaccaccgg
    57661 ttcactcagg ccgctgacct ggcagtggat tgtaaattcc tgatcgtgcg cttcgccacg
    57721 gacctgaact accagataag tcggcagcgg cagatgacga ccctgcaaat attcttgcaa
    57781 gcgcgttttc ggatcttttt gtttatcgcc tgggctaatt tcgtccagac gagtttgata
    57841 ccagttgagg attaatttct cgacggtttg aatatcactg tcgaggaata cgccaccaat
    57901 taatgcttcg acggtgtcgg cgagaattga ctcacgacga aatccaccgc ttttaagttc
    57961 acctggtcct aaacgtaagc actcgcctaa ctcaaattcg cgcgccagtt ccgccagcgt
    58021 attgccacgg accagcgtgg cgcgcatccg gctcatatcg ccttcatcca cacgagggaa
    58081 acggtgataa agcgcattgg cgataacgta gctcagaata gagtcgccta aaaattctaa
    58141 acgctcgtta tgtttgctgc tggcactacg atgagttaat gcctgctgca acagttcctg
    58201 atgattaaaa gtgtagccca gcttccgttg aagccgatta attacgatgg ggttcatgcg
    58261 ataccaataa ataaatgcgt caacaattca gcacacgaaa cagacctgat atacatggct
    58321 ctgctaactg cttcgctgca gtttcagttt agcggtatat ggggaccaac gctgtttcgt
    58381 gtgccgtggc aacctggagg tgccaacctt aaacttcggg ggaatattct atacacaacg
    58441 acgggggatg tcgttagcca cgggagattt atctcataaa taattcacgt tgtcgccata
    58501 acggcgacaa cgtgaacgaa gatgagctat taatggatgc cgccaatgcg acttaagcgc
    58561 acacctgtcg gccattcgcc ttcttgctta tcgaagctca tccagatagc cgttgcccga
    58621 ccgaccagat tcgcttcagg cacaaagccc cagtaacggc tgtccgcgct gttgtcgcgg
    58681 ttgtcgccca tcatgaagta ttgtcccgga ggaacaatcc aggttgccag ttgttgccct
    58741 ggctgctggt aatacatccc cacctgatcc tgcgcaatcg gcactgtcag aatgcggtgc
    58801 gtcacatcac ccagtgtctc tttacgctcg gaaagacgaa ttccattttc tttggtttcg
    58861 tttttcggca cttcaaagaa tccgctggtc gcttccccac cattacggcg tgagaaggtc
    58921 tgaacgaaat cgctcggttc cacgtttgag taggtgaccg gcagcgcgtt ttcacacgcc
    58981 tggccggaac tgcatcccgg ttgaatcgtc agctcttttg agaccggatc gtaagtgact
    59041 ttatcgcccg gtaaacccac cgcgcgcttg atataatcaa gctttggatc ttccggatat
    59101 ttaaagacca cgatatcgcc gcgtttcgga tgaccggttt cgatcagcgt tttctggtag
    59161 ataggatctt taatgccata agcaaacttc tctaccagaa taaaatcacc gattaacaga
    59221 gtcggcatca tcgaacctga cggaatctgg aacggttcat aaataaacga acgcacaatc
    59281 aatacgatag ccagtaccgg aaaaacagaa gcaccggttt ccagccagcc aggcttcggc
    59341 gcaacctttt tcaacgttgc tttatccagt gagtcaccgg cagccgcctg cgccgctgcc
    59401 tgacgttccc gccgtttagg tgcgaaaaag aatttatcca cgcaccataa aatgcccgtc
    59461 accagtgtgg caatcaccag aatcagggca aacatattcg ccatgccaac tcctaagggt
    59521 tatttgttgt ctttgccgac gtgcagaatg gcgaggaacg cttcctgcgg cagctcgacg
    59581 ttaccgatct gcttcatgcg tttcttacct tctttctgct tctgcagcag ctttttctta
    59641 cggctgatat cgccgccata acatttagcc agtacgtttt tacgcagctg tttcacggtg
    59701 gatcgcgcaa taatgtgcgt accaatcgct gcctgaatgg cgatatcaaa ctgctggcgt
    59761 gggatcagat ctttcatctt ctccaccaac tcgcgaccgc ggttttgcga attatcacgg
    59821 tgggtgatca acgccagcgc atcaacacgt tcaccgttga ttaatacgtc tacacgtacc
    59881 atgtcggacg cctggaagcg tttgaagttg taatccagag acgcataacc acgcgaggta
    59941 gatttcaggc gatcgaagaa gtcgagcacc acttccgcca tcgggatctc gtacgtcagc
    60001 gccacctgat taccgtggta aaccatattg gtctgcacgc cgcgtttttc tacgcacagc
    60061 gtaataacgt tgccgagata tgcctgcggc agcagcatgt gacactctgc aatcggctcg
    60121 cgcagttcgt aaatgttatt taccgcaggc agcttggatg ggctgtcgac gtagataact
    60181 tcccttgacg tggtttcaac ttcatacact acggtcggcg cagtggtgat cagatccaga
    60241 tcgtattcac gttccagacg ctcctggatg atctccatgt gcagcaggcc aaggaagccg
    60301 cagcggaaac caaagcccag cgcgctggag ctttccggct catagaacag tgaggcatcg
    60361 ttcaggctga gtttacccag cgcgtcacgg aaggcttcat agtcgtcgga acttaccggg
    60421 aacagaccgg cgtatacctg cggtttgact ttcttaaagc caggcagcgc cttttctgcc
    60481 ggattacgcg ccagcgttaa ggtatcgccg actggagcgc cgtggatatc tttaatcgca
    60541 catacgagcc agcctacttc gccacatttc agttcagtgc ggtcaacctg tttcggcgtg
    60601 aagatgccca gacggtcggc gttataggtc tgcccggtac tcatgacttt cactttgtcg
    60661 cccttacgca gggtgccgtt tttaatacgg ataagtgaaa caacgcccag gtagttgtcg
    60721 aaccatgagt cgataattag tgcctgcaac gggccttccg gatcgccttc cggcggcgga
    60781 atgtcgcgca ccagacgttc gagaacgtcc tgcacgccaa cgccggtttt cgctgaacag
    60841 cgcaccgcgt cagtggcgtc gatgccgacg atatcttcaa tttcttccgc cacgcgttca
    60901 ggatcggctg ccggcaggtc aatcttgttc agtaccggca ccacttcgag atccatttcc
    60961 atggcggtgt agcagtttgc cagggtttgc gcttccacgc cctgcccggc gtcgaccacc
    61021 agcaatgcac cttcacaggc agccagcgaa cgggaaactt cataggagaa gtctacgtgg
    61081 cccggggtgt cgataaagtt aagctgatag gtttcgccgt cagacgcttt gtagtccagc
    61141 gtcacgcttt gcgctttgat ggtaatgcca cgctcacgct caagatccat ggaatcgaga
    61201 acctgcgcct ccatttcacg gtcagacagg ccaccgcaga tctggataat acggtcagac
    61261 agcgtcgatt taccgtggtc aatgtgagct atgatcgaaa agttacgtat attcttcata
    61321 aagtatgatt attgtgcctt aatgcccggt taaccaggct tttagaagtc gctgttctga
    61381 gcttaacgtc tgtattaata gaaacgccgc attctacact acaacattga ggcgaggaaa
    61441 tgttcatacc gtatggattg tggtatctgg aaacgtcctc gcatttgtta tgcaaaatgc
    61501 aacaaagcca gtgaaatcac tggctcgcgt cttccgaaga tgtttcaaat cgcacaaggc
    61561 caggcggcaa tgccacgctt aagatgatcg gttgccattc tgcccgggca gcaaacttac
    61621 gcgagtagcc acgcgcaatc aggaacccgc caataccgcc gaggaccgca ccacataatg
    61681 ctgcgacgtc agaagcaaag agtagctgaa atagcgaagc gatgaggaat aatcccacca
    61741 gcggcgacat ataaaccagt aatgcggagc taagcaggct gccttcggcg atccccaatt
    61801 ccactttttg ccccggcact aacggctcat cacagggtac gacaatggta tgcgtggttt
    61861 gcgggccaag tttattcaac acgcggctac cgcaaccggc gcgtgaagcg cagctgctgc
    61921 atgaggcttt aacatcacaa ctgaccagcg cctgcccgtt ttgccaggag acgacggtag
    61981 cccactcttt gatcattgcg ctgccccgaa cttaatattc tcggcaatgc gtttcgccgt
    62041 ttgcggcggc agttcaccga caatggtgat ttcggcgtta tcacgtacgc ttgtactgac
    62101 ggttctgcgt ccggtgcgca acatctgatc ggtgctcgat ggcgtagcgc ggttaacgtt
    62161 taccgagaag ctgaataatc cgtcggaata gagacgtgat tcgataggca tgttgtccat
    62221 cgtcggtagc ggacgtcgac tactggaaac ttcgctaaaa ccctgtggca accaggttgg
    62281 cgtccagctg aatttagctt tttcacctac aggaacagaa agcaacggcg gcaaatttgc
    62341 cttcgccagc gtctgcatac tgctgctgat atcctgattg acgttaaaag caatcacgcg
    62401 aaattgttcc agcgtttcac catcgcgatc aagaagatca acccgcatcg gtaatttcga
    62461 ttcggtgtcc atccacacga tgtagctgta gcgtgtacca tctcgggcaa ccacgcgaat
    62521 gacttcgcaa agacgatcag caatacgcgt gcgcccgacg gagataaagt cgtagtaagg
    62581 agaaaggcgt ttgaaatcgg tatagatgag cgatggcaga gaatcaacaa tgtaatcgcc
    62641 attaagcgtg aacggttcaa gtcccggttc aaaatagctg atttcattgc cgcgctgtac
    62701 cacttcccgg cgcgggccat ccatttgcaa caattgtgca agaggacggt tatcgaggcg
    62761 tgcatgtcga taacgcagag actcaacacc ctgtttattg atgctgatga atgacagctc
    62821 gtaattcagt gactgactgg ccaggttcat ctgctgtaat aacgccccgg acgcgggagt
    62881 ggccgaggcg ttagcagaga ataacaggct acctgtcact aatgacatgg caaaccaaag
    62941 ttgcttcatt actgcgattg cgttcctaaa gtttgaattc ctggcacctg tacagcggct
    63001 tgctgggttt gcgcctgctc aaactgaagc tgttcagagt ggagtcggcg ttgcagttcg
    63061 taatcctgca acattgcatt aatgcgacga cgctgctgct gttgaccatt atttgcggtc
    63121 gcttcagaag gtactcccag gctaaccggg ctggctttac ccatcatcgg cagtgtatta
    63181 aataccggcg tttcgggctg ctgggacgtt tcagattgtc cattatagtg ctggacgcca
    63241 acgataactg caagcgatac gcatgcggct acgcccattt gggtaagctg tgccgcccac
    63301 ggacgtactt tctgccagaa tggcattttc tgccattcat gcggcgcagg ctgggcttcc
    63361 gggatcaatg tcgccggttg acgtactggc tcttcttcaa tggcggccat cacgcgtgaa
    63421 gagatatcga aatggagcac ctcgggagta tcaccccgca ttgagtcacg gattaagtga
    63481 tagctttccc aggttttctg catttctggg ttatgagcca gttcgttaag cagctcacta
    63541 tccagcgttt cgccatccat taaagcggaa agttgttctt tctgcatgcc taataccctt
    63601 atccagtatc ccgctatcgt caacgcctga taagcggttg aactttgtta tcaatagctt
    63661 ccctcgctcg gaagatacgt gaacgcaccg tacctaccgg acaatccatg atagcggcta
    63721 tctcttcata gctcaggcca tccagctccc gcaaggttat tgccatgcgt aaatcttccg
    63781 ggagggactc aatagttcgg aaaactatct gtctcagttc ttctgacaac attaagttct
    63841 cagggttcga aatttctttc aacgcgccgc cactttcgaa gttttcagct tcaatggcat
    63901 ccacatcact ggaaggtgga cgacgcccct gagcaaccag gtaatttttc gctgtattta
    63961 cagcaatccg atacagccat gtataaaaag cgctatctcc ccggaacgaa tccagcgcac
    64021 gataggcttt aataaaagct tcttgtacca catcgggaac atcacccgac agcacatagc
    64081 gggaaaccag actcgccact ttatgctgat agcgcactac cagtaagtta aaggctttct
    64141 gatctccctt ctggacccgt tcaaccagga cctggtccgt taactgctcg ctcatccgag
    64201 gtaaagtctc cccaaaccaa atttccacgc gctatcgaaa cgccactcca ttagctgcaa
    64261 tttgagcaag caaagggtta gagtgtctcg tttttgtaaa gttccgtaac gcatctgttt
    64321 ttgtttgtca tgctgtagac ggatcattat ctatcattat aagtctacag aatctgaaca
    64381 tcgcattatc tgtgtagaaa tgcccattta actgcctgaa gagtaaccca acggcctttt
    64441 tatttcacca cctaatcctc caccagccag taacttctct ttttctcgcc gccctgcgtc
    64501 agcgtgttta gtcactgtaa caaatattaa aatagcaggt gtttattcgc acaacatgat
    64561 gctatgctga ccaaaccgtg tttagtaaat taaacaaaga aaatgaatac tctccttgaa
    64621 cattcatgtg acgtgttgat tatcggtagc ggcgcagccg ggctttcact ggcgctacgc
    64681 ctggctgacc agcatcaggt catcgttcta agtaaaggcc cggtaacgga aggttcaaca
    64741 ttttatgccc agggcggtat tgccgccgtg tttgatgaaa ctgacagcat tgactcgcat
    64801 gtggaagaca cattgattgc cggggctggt atttgcgatc gccatgcagt tgaatttgtc
    64861 gccagcaatg cacgatcctg tgtgcaatgg ctaatcgacc agggggtgtt atttgatacc
    64921 cacattcagc cgaatggcga agaaagctac catctgaccc gtgaaggtgg acatagtcac
    64981 cgtcgtattc ttcatgccgc cgacgcaact ggtagagaag tagaaaccac gctggtgagc
    65041 aaggcgctga accatccgaa tattcgcgtg ctggagcgca gcaacgcggt tgatctgatt
    65101 atttctgaca aaattggcct gccgggcacg cgacgggttg ttggcgcgtg ggtatggaac
    65161 cgaaataaag aaaaggtgga aacctgccac gcaaaagcgg tggtgctggc aaccggcggt
    65221 gcgtcgaagg tttatcagta caccaccaat ccggatattt cttctggcga tggcattgct
    65281 atggcgtggc gcgcaggctg ccgggttgcc aatctcgaat ttaatcagtt ccaccctacc
    65341 gcgctgtatc acccacaggc acgcaatttc ctgttaacgg aagcactgcg cggcgaaggc
    65401 gcttatctca agcgcccgga tggcacgcgt tttatgcccg attttgatgt gcgcggcgaa
    65461 ctggccccgc gcgatattgt cgcccgcgcc attgaccatg aaatgaaacg cctcggcgca
    65521 gattgtatgt tccttgatat cagccataag cccgccgatt ttattcgcca gcattttccg
    65581 atgatttatg aaaagctgct cgggctgggg attgatctca cacaagaacc ggtaccgatt
    65641 gtgcctgctg cacattatac ctgcggtggt gtaatggttg atgatcatgg gcgtacggac
    65701 gtcgagggct tgtatgccat tggcgaggtg agttataccg gcttacacgg cgctaaccgc
    65761 atggcctcga attcattgct ggagtgtctg gtctatggct ggtcggcggc ggaagatatc
    65821 accagacgta tgccttatgc ccacgacatc agtacgttac cgccgtggga tgaaagccgc
    65881 gttgagaacc ctgacgaacg ggtaataatt cagcataact ggcacgagct acgtctgttt
    65941 atgtgggatt acgttggcat tgtgcgcaca acgaagcgcc tggaacgcgc cctgcggcgg
    66001 ataaccatgc tccaacaaga aatagacgaa tattacgccc atttccgcgt ctcaaataat
    66061 ttgctggagc tgcgtaatct ggtacaggtt gccgagttga ttgttcgctg tgcaatgatg
    66121 cgtaaagaga gtcgggggtt gcatttcacg ctggattatc cggaactgct cacccattcc
    66181 ggtccgtcga tcctttctcc cggcaatcat tacataaaca gataaaaagc ctgggttagc
    66241 gccgtatacg cttcggaata gttctggtct ggcccacgaa tgactaagcg atcgctaaag
    66301 cattctcccg cctgcgggga gaatgccagc agcacccgat gcggcagtcg cgcttcgttt
    66361 tccgccacat ccgtccgcaa acgtaaatgc cagcccatac ttaatgccag ctccgtaaaa
    66421 ctattaccaa tctgctctgg cagcaccacg cagaaaaatc cctcttcggt aatgcactcc
    66481 gccgcgcaag taagtaaagt ctggtgatca agtgaggtgg tgtagcgcgc ctgttcccgc
    66541 tgcggcgtcg cgcactccac tccttgctca taatacggcg ggttgctgat gattaaatca
    66601 aagcgcgcgg tctgctgtgt tacccactgc tgaatatccg ccgtatggac gttaattctc
    66661 tctaaccacg gggactgggc gacattttcc tgcgcctgag tggcagcttc actttccagc
    66721 tcaacggcat caattatcac gctgtcactg gttcgctgcg ccagcatcag tgccagcaag
    66781 ccactacccg caccgatatc aaggcaacgt tttaccccag ccaccggtgc ccatgcgccc
    66841 agcaatatgc catccgttcc cactttcatc gcacagcgat cgtgagcaac aaaaaactgt
    66901 ttaaaagtaa atccattacg acgaagcacg gatgtagact gtgacatgaa aataaaacct
    66961 tgcaggaaaa acggcgatag caccgggtga gaacaatacc cgagaagcga tatccataca
    67021 aacagatgaa gattgcagcc gtaacgtcta taatcagcgc cccacacaga ggtagaacat
    67081 gactgtaacg actttttccg aacttgaact cgacgaaagc ctgctggaag ccctccagga
    67141 taaaggtttc actcgcccga ccgccattca ggctgccgcc attccgcctg cgctcgatgg
    67201 ccgtgatgta ctcggttctg cgccgacagg caccggtaaa acggcggcgt atctgctgcc
    67261 agcgttgcag cacctgctcg atttcccgcg taagaaatcc ggtccgccgc gtattttgat
    67321 cctcacccca actcgcgagc tggcgatgca ggtggccgat catgcccgcg aactggcgaa
    67381 acatacgcat ctggatatcg ccaccatcac cggcggcgta gcctatatga accacgcgga
    67441 agtgttcagc gaaaatcagg acatcgtggt cgccacgacc ggacgtctgc tgcaatacat
    67501 aaaagaagag aacttcgatt gccgcgcggt tgaaacgctg atcctcgacg aagcagaccg
    67561 tatgctggat atgggcttcg ctcaggatat cgaacatatt gctggcgaaa cgcgctggcg
    67621 taaacagacc ctgctctttt cggcaacgct ggaaggcgat gcgattcagg actttgccga
    67681 gcgtctgctg gaagatccgg tggaggtttc tgccaatccg tccacccgtg agcgcaaaaa
    67741 aattcatcag tggtattacc gcgccgatga cctggaacat aaaaccgcat tgctggtgca
    67801 tctgttaaaa cagccggaag cgacccgctc aattgtgttt gtgcgtaagc gtgagcgtgt
    67861 gcatgagctg gcaaactggc tgcgcgaagc gggcatcaac aactgctatc tcgaaggtga
    67921 gatggtacag ggcaagcgta acgaagcgat caaacgtttg accgaaggtc gcgtaaacgt
    67981 actggttgca accgatgttg ccgcgcgcgg tatcgacatt cctgacgtca gccacgtctt
    68041 taacttcgat atgccgcgca gtggcgatac ttatttgcac cgtatcggac gtaccgcgcg
    68101 cgccggtcgt aaaggcaccg caatttcgct ggtagaagcc catgaccatc tgctgctggg
    68161 caaagttggt cgctatattg aagagccaat taaagctcgc gtaattgatg agttacgccc
    68221 gaaaacgcgt gcaccaagcg agaagcagac cggcaagcca tcgaagaaag tactggcaaa
    68281 acgcgccgag aagaaaaaag ctaaagagaa agagaagccg cgggtgaaaa aacgccatcg
    68341 cgataccaaa aatattggta agcgccgtaa accaagcgga acgggcgtgc caccgcaaac
    68401 gacagaagag taatctcaat gccgggttta agcccggcat tatttttatc ccggcaaaaa
    68461 acaatcttca acacactgaa taaaagtgtg catcgccggg ctaaccgctt ttccggcatg
    68521 gtgagcgcac attgccgtaa tggtctgcga gtgttcgcca aaaggcaatt caattaattc
    68581 tccgctctca agctcttttt ccaccgcaaa gcggggcaag taactgacac cgatattcgc
    68641 cgcgacacaa cgtttgatac tttcaatgct taacagctca atggtgtttt ccaccgtgat
    68701 ccgccgctgg cgcagcgtgc tctcaaatat ctgccggaat acacattgcg gttcgttgat
    68761 aataaagcta caggcgttat gtcttcccgg ctcagtaaaa tcgacatctg caatttgcgg
    68821 tgaagctacc agcaccagcg attgctcacc caattctcgt cggttcaggg catcatcatt
    68881 ccctacgcga taaaaaaccc cgacatcggc ctcatcattc aatagcgcat cacggatcac
    68941 gtaacagttc agagactgca acgataaacg cacttttggg gcgcgttgcc gaaaacgctg
    69001 caacacctgc ggcattcggt aagagagcaa cgtttcgccc gaaacaacgc gtagttcccc
    69061 gtccggatcc gactcctttt tggcagcctc acgaagggta tccatcactc tggtgagttc
    69121 ataaatgtgc ggcagcagct ttttaccttc gcgagtgagg cacatccgtc ggccaatttt
    69181 ctcaaataac tgtaccgaga actcctgctc aagctgctga atatgaaaag tcaccgtcga
    69241 ttgtgtacag cacaattttt gcgaagctcg caaaaaggaa ccctcttcca ccacggtttt
    69301 aagcgtaata aaacggcgca gatccataac cccaaaccta tcgaaaatat cgaatttaga
    69361 atgtaaaaac attcattttt ttaaatattc tgtgtcgggt actgtctacc aaaacagagg
    69421 agataacaag tgacaccgac ccttttaagt gctttttgga cttacaccct gattaccgcc
    69481 atgacgccag gaccgaacaa tattctcgcc cttagctcag ccacaacgca cggatttcat
    69541 cagagtaccc gcgtgctggc agggatgagt ctgggatttt taatcgtcat gttgctgtgt
    69601 gcgggcattt cgttttcact ggctgttatc gacccggcag ctgtgcatct gttgagttgg
    69661 gcgggggcgg catatattgt ctggctggcg tggaaaatcg ctaccagtcc aacaaaggaa
    69721 gacgggcttc agacaaaacc aatcagtttt tgggccagct ttgctttgca gtttgtgaac
    69781 gtcaaaatca ttttgtacgg tgttacggca ctgtcgacat ttgttctgcc gcaaacacag
    69841 gcgttaagct ggatagttgg cgtcagcgtt ttgctggcaa tgattgggac gtttggtaat
    69901 gtgtgctggg cgctggcggg gcatctgttt cagcgattgt ttcgccagta tggtcgccag
    69961 ttaaatatcg tgctggcgct gctgctgatc tattgcgcgg tacgcatttt ctattaatga
    70021 aaaaaagcgg aagaggtcgc cctcttccgc ttagtaacat gctacttaag ccttacaggc
    70081 tttcagtaaa ggtacgagcg ataacgtcgc gctgctgttc cggagtcaga gagttaaagc
    70141 gaactgcata accggataca cggatggtca gctgcggata tttttccgga tgcttaactg
    70201 catcttccag agtttcacga cgcagaacgt taacgttcag gtgctgacca ccttcaacgc
    70261 gaacttctgg tttcacttct actggaactt cacggtactc aatgtcaccc agtttgctta
    70321 ctgcaaccac ttcgtcttct gcataacctg ctttcgcaac gatgcaacgc gcttcgcctt
    70381 tttcgctgtc cagcagccag aaagagttca gcagatcgtc gttagcggct ttagtaatct
    70441 ggatacctgt aatcatgtga tgcctccccg gcaaaattat ttgatttgtt cagcctgtcg
    70501 cggccaattg gtaaaaccat tgttgcttga gtgtatatat actcctcaaa cacccttgaa
    70561 tctttgattt aaatcaataa aaaccacaca tcaagtgtgg tcgcaaatag attttattgt
    70621 tttacatcaa cttatgcggg tgtgaaattt taccaattta catttttttg cactcgttta
    70681 agtctaaaaa atgagcatga ttttgttctg tagaaagaag cagttaagct aggcggattg
    70741 aagattcgca ggagagcgag atggctaacg aattaacctg gcatgacgtg ctggctgaag
    70801 agaagcagca accctatttt cttaataccc ttcagaccgt cgccagcgag cggcagtccg
    70861 gcgtcactat ctatccacca caaaaagatg tctttaacgc gttccgcttt accgagttgg
    70921 gtgacgttaa agtggtgatt ctcggccagg atccttatca cggaccggga caggcgcatg
    70981 ggctggcatt ttccgttcgc cccggaatcg ccactcctcc gtcactgctg aatatgtata
    71041 aagagctgga aaatactatt ccgggcttca cccgccctaa tcatggttat cttgaaagct
    71101 gggcgcgtca gggcgttctg ctactcaata ctgtgttgac ggtacgcgca ggtcaggcgc
    71161 attcccacgc cagcctcggc tgggaaacct tcaccgataa agtgatcagc ctgattaacc
    71221 agcatcgcga aggcgtggtg tttttgttgt ggggatcgca tgcgcaaaag aaaggggcga
    71281 ttatagataa gcaacgccat catgtactga aagcaccgca tccgtcgccg ctttcggctc
    71341 atcgtggatt ctttggctgc aaccattttg tgctggcaaa tcagtggctg gaacaacatg
    71401 gcgagacgcc gattgactgg atgccagtat taccggcaga gagtgagtaa atttgcggga
    71461 aaatgccgga tggcagagtt gccacccggc tgatttatca ggctttattc tgacgccacc
    71521 attcgccaag aagaacgccg gttgcgacag agatattcag cccggcaacg ttgccagtac
    71581 catcaatctt cacgcgcaga tcgttcggat cgcgtgcggc atccggtaac ccttcatatt
    71641 cctgacccag caccagtacc attttcgctg gcagactggt tttgaacagc ggtttaccct
    71701 gctcgctgga agttgtcact acggtgtaac ccgcctgacg gaaatcatcc agcacgttaa
    71761 caatgttgtc gccagtaatc ggctgaacgt gctctgcgcc gccttctgcg gtacggatag
    71821 ccgcccccga ctccagcagt gccgcatcct gcaccacaac acctttcacg ccaaagtgcg
    71881 cgcagctgcg catcatgcca cccaggttat gcgggttaga ttcgttttcc agtgccagaa
    71941 cacaatcctg cgcgcctgcc tggcttaccc actgctgcac ggttgtgcca ttacgctttt
    72001 tgatcaggaa gcaaacgcca ccgtgatgtt ccgtgcctga cgcttttgtc aattccgctt
    72061 catctaccac atggtacgct ttgcggtttg ctgccatcca gcgcaaggct tctttaaaac
    72121 gcggcgttac actctggata aaccaggcgc gaacaatcgc ttccggacgg ctctggaaca
    72181 gtgcctgaca ggcgttttcg ccgtagacgc gggtttcttc cgcacgctga cgacgcaaca
    72241 cttccggatc aataaaactt ttaccactga tgccaccgtg atcggccttt tccggcgtct
    72301 catcacccgg cgcgcgggaa accgtgcgcc acggcgaatc ttcccacttg cggtcacggg
    72361 gctgactctg tttgtcatcg cgggcggggc gacggccacc gtcagcacga gattttcctg
    72421 gacgcccgcc ccctttcccg gtacgcgggt tgtgggtacg tttatcagaa tcatcatcac
    72481 tgcggacata catcactttg accttgccgc ttttaccttt catttcatcg ttcatgcttt
    72541 tctccaccag cgctgcgcga agcgcgcaga ttacccgaag tccgcgcggt tcgccatgat
    72601 ttcgtaccaa agcctgcgac tatcatacct attgaataaa acagattgtt gtctggaaca
    72661 atgtccccga taatatgtaa catattagaa acataccggc gttgttgccg ataagtctcc
    72721 ttactcatcc cgaggttagt tatgaatacc gtttgtaccc attgtcaggc catcaatcgc
    72781 attcccgacg atcggatcga agatgcggca aaatgcggac gctgcggtca cgacttgttt
    72841 gacggagagg tgattaatgc gaccggtgaa acgctcgaca aattgctgaa agatgatcta
    72901 ccagtggtga tcgacttctg ggcaccgtgg tgcggcccct gccgtaattt cgcaccaatt
    72961 tttgaagatg tcgcgcaaga gcgtagcggt aaagtgcgct ttgtgaaagt gaataccgaa
    73021 gctgaacgtg aattgagcag tcgctttgga attcgtagta taccgacgat catgattttc
    73081 aaaaacggtc aggttgtcga catgcttaat ggcgcagtac cgaaagcgcc gttcgatagc
    73141 tggctgaacg aatctcttta atcttaccgg ggcgcatctt gtgccccgtt ttctcctctg
    73201 cgacaatggc gttttttcga cgctctctta tgaccgaaaa cgctgttctc cagttacgcg
    73261 ccgagcgtat tgcgcgcgca acacgtcctt ttcttgcccg cggtaatcgc gttcgtcgct
    73321 gccaacgctg tcttttgcca gagaaattat gtctctgttc gacaattacc ccagcacaag
    73381 caaaaagtcg cttctgtttg ctgatgttcg acaccgagcc aatgaagccc agtaataccg
    73441 ggcgtctgat tgctgatatt ttgcctgata ccgttgcgtt tcaatggtcg cgtaccgaac
    73501 cctcgcagga tttgctggat ctggtacaaa acccggatta tcagccaatg gtggtctttc
    73561 ccgcctcgta tgctgatgag caacgggaag tgatcttcac accacctgcc ggtaagccac
    73621 cgctgtttat catgctcgat ggtacctggc cggaagctcg caagatgttt cgtaaaagtc
    73681 cgtatctgga taatcttccc gtcatttccg tcgatctttc ccggctttct gcctatcgcc
    73741 tgcgtgaagc ccaggctgaa ggccaatatt gtactgccga ggtagccatc gcactgttag
    73801 atatggccgg cgataccggg gcggcagcag ggttaggcga gcacttcacc cgctttaaaa
    73861 cgcgctatct ggcaggaaaa acgcaacatc tgggtagcat cacagcagaa cagttagaaa
    73921 gcgtttaaaa tcattcggtc acttctgcgg gagaccggta tgagtcagcg aggactggaa
    73981 gcactactgc gaccaaaatc gatagcggta attggcgcgt cgatgaaacc caatcgcgca
    74041 ggttacctga tgatgcgtaa cctgctggcg ggaggcttta acggaccggt actcccggtg
    74101 acgccagcct ggaaagcggt gttgggtgtg ttggcctggc cggatattgc cagcttgccc
    74161 tttacacccg accttgcggt tttatgtacc aatgccagcc gtaatcttgc tcttctggaa
    74221 gagctcggcg agaaaggctg taaaacctgc attattcttt ccgccccggc atcgcaacac
    74281 gaagatctcc gcgcctgcgc cctgcgccat aacatgcgcc tgcttggacc aaacagtctg
    74341 ggtttactgg ctccctggca aggtctgaat gccagctttt cgcctgtgcc gattaaacgc
    74401 ggcaagctgg cgtttatttc acagtcggct gccgtctcca acaccatcct cgactgggcg
    74461 caacagcgtg agatgggctt ttcctacttt attgcgctcg gcgacagcct agatatcgac
    74521 gttgatgaat tgcttgacta tctggcacgc gacagtaaaa ccagcgccat cctgctctat
    74581 ctcgaacagt taagcgacgc gcgacgcttt gtttcggcgg cccgtagtgc ctcgcgtaat
    74641 aaaccgattc tggtgattaa aagcggacgt agcccggcag cacagcgact gctcaacacg
    74701 acggcaggaa tggatccggc gtgggatgcg gcgattcagc gtgccggttt gttgcgggtg
    74761 caggataccc acgagctgtt ttcggcggtg gaaaccctca gccatatgcg cccgctgcgt
    74821 ggcgaccggt tgatgattat cagcaacggt gctgcgcctg ccgcgctggc gctggatgcc
    74881 ttatggtcac gcaatggcaa gctggcaacg ctaagcgaag aaacctgcca gaaactgcgc
    74941 gatgcactgc caggacatgt ggcaatctct aacccgctcg atctgcgcga tgacgccagc
    75001 agcgagcatt atgtcaaaac gctggacatt ctgctccaca gccaggattt tgatgcgctg
    75061 atggttattc attcgcccag cgccgctgct cccgcaacag aaatcgcgca agtattaatt
    75121 gaagcggtaa agcatcatcc ccgcagcaag tatgtttctc tgctgacgaa ctggtgcggc
    75181 gagcactcct cgcaagaggc acgacgttta ttcagcgaag ccgggctgcc gacctaccgt
    75241 accccggaag gaaccatcac tgcttttatg catatggtgg agtaccggcg taatcagaag
    75301 caactacgcg aaacgccggc gttgcccagc aatctgactt ccaataccgc aaaagcgcat
    75361 cttctgttgc aacaggcgat tgccgaaggg gctacgtcgc tcgataccca tgaagttcag
    75421 cccatcctgc aatcgtatgg gatgaacacg ctccctacct ggattgccag cgatagcacc
    75481 gaagcggtgc atattgccga acagattggt tatccggtgg cgctgaaatt gcgttcgccg
    75541 gatattccac ataaatcgga agttcagggc gtcatgctgt acctgcgtac agccaatgaa
    75601 gtccagcaag cggcaaacgc tattttcgat cgcgtaaaaa tgacctggcc gcaggcgcgg
    75661 gtccacggcc tgttggtgca aagtatggct aaccgtgctg gcgctcagga gttgcgggtt
    75721 gtggttgagc acgatccggt ttttgggccg ttgatcatgc tgggtgaagg cggtgtggag
    75781 tggcgtcctg aagatcaagc cgtcgtcgca ctgccgccgc tgaatatgaa cctggcccgc
    75841 tatctggtta ttcaggggat caaaagtaaa aagattcgtg cgcgcagtgc gctacgccca
    75901 ttggatgttg caggcttgag ccagcttctg gtgcaggttt ccaacttgat tgtcgattgc
    75961 ccggaaattc agcgtctgga tattcatcct ttgctggctt ctggcagtga atttaccgcg
    76021 ctggatgtca ctctggatat cgcgccgttt gaaggcgata acgagagtcg gctggcagtg
    76081 cgcccttatc cgcatcagct ggaagaatgg gtagaattga aaaacggtga acgctgcttg
    76141 ttccgcccga ttttgccaga agatgagcca caacttcagc aattcatttc gcgggtcacc
    76201 aaagaagatc tttattaccg ctactttagc gagatcaacg aatttaccca tgaagattta
    76261 gccaacatga cgcagatcga ctacgatcgg gaaatggcgt ttgtagcggt acgacgaatt
    76321 gatcaaacgg aagagatcct cggcgtcacg cgtgcgatct ccgaccctga taacatcgat
    76381 gccgaatttg ccgtgctggt tcgctcggat ctcaaagggt taggcttggg tcgacgctta
    76441 atggaaaagt tgattaccta tacgcgagat cacggactac aacgtctgaa tggtattacg
    76501 atgccaaaca atcgtggcat ggtggcgctg gcccgcaagc tcgggtttaa cgttgatatc
    76561 cagctcgaag aggggatcgt tgggcttacg ctaaatcttg cccaacgcga ggaatcatga
    76621 gtaaggtact ggaaatgttg accacttaat cgggactggt gttattattg cacgcttatg
    76681 tcgtctgcat tgcacagagg acccttcaat gaacagagaa gaaatgcact gtgatgttgt
    76741 caaaatttaa gcgtaataaa catcaacaac accttgccca actacccaag atttctcaat
    76801 cagttgatga tgtcgatttc ttttacgctc ccgccgactt ccgggagacg ctgctggaaa
    76861 aaatagccag cgcgaagcag cgcatttgca ttgtcgccct gtatctcgaa caggatgacg
    76921 gtggcaaagg cattctgaac gcgttgtatg aagctaaaag gcagcgtccg gaactggatg
    76981 tgcgggtgct ggtcgactgg catcgtgcac aacgtggacg cattggcgct gcggcatcta
    77041 acactaacgc tgactggtac tgtcgcatgg cgcaggaaaa tccgggcgta gatgttccgg
    77101 tttatggcgt tccaatcaat acccgtgaag cccttggtgt tctgcacttt aaaggcttta
    77161 tcatcgacga tagcgtactt tatagcggtg ccagcctgaa cgatgtttac ctgcatcagc
    77221 acgataaata tcgctacgac cgttatcatc ttatccgtaa ccgtaagatg tcagacatta
    77281 tgtttgaatg ggttacacag aatattatga atggccgcgg cgttaatcgt ctggatgatg
    77341 ttaatcggcc aaaaagcccg gaaatcaaga acgatattcg tctgttccgc caggagctgc
    77401 gtgatgccgc ttatcatttc cagggcgatg ccgacaacga tcagctttcc gtaacgccgc
    77461 tagtggggct ggggaaatcg agtctgttga acaagaccat tttccatctt atgccttgtg
    77521 ccgagcagaa actaaccatc tgtacgccat acttcaacct gccagcaatc cttgtgcgca
    77581 atattatcca gttgctgcgc gaagggaaaa aggtcgaaat tattgttggt gataaaaccg
    77641 cgaacgactt ctacattccg gaagatgaac ctttcaagat aattggcgca ttgccttatc
    77701 tctatgagat caatctgcgt cgtttcctga gccgtttgca gtattacgtc aatactgacc
    77761 agctagtggt tcggttatgg aaagatgacg acaacaccta tcacctaaaa gggatgtggg
    77821 ttgatgataa gtggatgttg atcaccggta ataacctgaa cccgcgcgcc tggcgtctgg
    77881 atctggaaaa cgccattttg atccacgatc cacaactgga gcttgcgccg cagcgagaga
    77941 aagaactgga gctgatccgc gagcatacca ccatcgttaa gcactatcgc gatctgcaaa
    78001 gtattgccga ttatccggtg aaggttcgta aactcatccg ccgtttgcgc cgtatccgca
    78061 tcgaccgatt aattagccgc atcctgtaat cacaaccccg tcctgtacgg ggtttgtttt
    78121 ttggaggcca cgttttgcgt attctttttg tctgttcgct attgttactt tctggatgca
    78181 gccatatggc taacgatagc tggagcgggc aggataaagc tcaacacttt atcgcctcgg
    78241 cgatgctttc cgccgccgga aatgaatatt cacagcatca ggggatgagc cgggatcgca
    78301 gtgccatgtt tggtttgatg ttctctgtca gtttgggggc gtcaaaagag ttttgggata
    78361 gccgccccga agggagcggc tggagctgga aggatttggc ctgggatgtc gccggtgcaa
    78421 gcaccggcta taccgtctgg caactgaccc gtcactaaag acgcatcccc ttccctttgc
    78481 gatgtagcat caaagaaacc agaaacgcca ccacggccat caaggtcaca taccagaaga
    78541 aggctgtttc cattcctatt gatttcagcg acaacgctac gtactccgcc gaaccaccaa
    78601 atatagcatt agcgaccgca tatgacagac caacgcctaa tgcgcgaacc tgtgccggga
    78661 acatctcagc cttcagtatt ccactgattg atgtataaaa actcactatc agcagggcac
    78721 acatcaccag accaaaagcg gcataaggcg aggaaacgtt ttgcaatgct gagagaatag
    78781 gaacggtaaa aatggctgcc agcgaaccga aacataacat tgaggtacgg cgaccaatct
    78841 tatccgacag cgcgccaatg agtggttgaa taagcatgaa tacaaacaat gcggcagtca
    78901 taatgccact cgccacgttg gcatgcattc ccgcagtatt taccagatac ttctgcatat
    78961 aagtggtgaa ggtatagaaa caaagggagc ccgcagcggt aaaaccgaga accatgatga
    79021 atgcacggcg attgcgccat aatcctttca gagatccagc ttcttttaaa gcgcgcgttt
    79081 cttgttgcga agtttcatct aactgacgac gtaaccacaa cgccacaaca gctaacacag
    79141 ctcctaacgc gaaaggaata cgccatcccc actctctgag tgcagagtct tccatggtgt
    79201 gttgtaaaac cacgacaacc agtagggcta gcagttgtcc gccgatcaac gtcacatact
    79261 gaaatgatgc gtaaaaacct ttgcgccctt caacggcaac ttcactcata taggtggcgc
    79321 tggtgccata ttctccgcca acggataatc cctgaaataa acgcgcgaga agcaataatg
    79381 ccggagccca cgtaccgatt gtttcataac caggcaaaca ggcgattacc agtgagccga
    79441 aacacatcat acacaccgat agcagcatcg attttttgcg accatgttta tcggctatgc
    79501 ggccaaatag ccaaccgcct attgggcgca tcaggaatcc cgcagcaaaa acacctgctg
    79561 tttgtagtag ttgagtcgtc gtgttcccgg aagggaagaa gatgtgggca aagtagagtg
    79621 aacagaacga gtagacatag aaatcgaacc actcgaccag attacctgaa gaggccccca
    79681 caatcgccca aatgcggcga cgagtatcac tacttgtcag tttgctgtct gccgttacag
    79741 tactttcagc catgccatta tgtctcctgc cgtaatccga tgcttttgtc ggtcgctttt
    79801 gtttatttct tttggcaaag aaaatgttat acatttgtta cgtatcatta tgcaacctta
    79861 accatgaatt tagttagcag gaaaatggtt attgaggagc ttaaggataa atttctggta
    79921 aggaggacac gtatggaagt aggcaagttg gggaaaccgt atccgttgct gaatctggca
    79981 tatgtgggag tataagacgc gcagcgtcgc atcaggcatt tttttcacca acgcaaaaag
    80041 gccatcctga cggatggcct tt
//
LOCUS       DAZFBR010000010        66436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFBR010000010 DAZFBR010000000
VERSION     DAZFBR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.010"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..1607)
                     /locus_tag="ACHJZ2_000812"
     CDS             complement(<1..1607)
                     /locus_tag="ACHJZ2_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4566980.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFTEGPAI
                     ISGVNNNDRLYMLKKDFNSAISQASLTVTTPMISLYPYVISNVFYILPLSILLSFILY
                     FLWQRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGKNI
                     SPVVFIRAAEEENLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVAD
                     VLHIKNAISDKFNIVLEITERNLVEDTDHALQKINELRSHGCEFAVDDFGTGYCSLGL
                     LQKLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGVSTSHQAEYLI
                     HNNVTLVQGYLYAKPMKATEFYQWYVN"
     gene            complement(1705..3063)
                     /gene="dgcT"
                     /locus_tag="ACHJZ2_000813"
     CDS             complement(1705..3063)
                     /gene="dgcT"
                     /locus_tag="ACHJZ2_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO4566981.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            3650..6073
                     /gene="pgaA"
                     /locus_tag="ACHJZ2_000814"
     CDS             3650..6073
                     /gene="pgaA"
                     /locus_tag="ACHJZ2_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO4566982.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            6082..8100
                     /gene="pgaB"
                     /locus_tag="ACHJZ2_000815"
     CDS             6082..8100
                     /gene="pgaB"
                     /locus_tag="ACHJZ2_000815"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO4566983.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            8093..9418
                     /gene="pgaC"
                     /locus_tag="ACHJZ2_000816"
     CDS             8093..9418
                     /gene="pgaC"
                     /locus_tag="ACHJZ2_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO4566984.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            9420..9833
                     /gene="pgaD"
                     /locus_tag="ACHJZ2_000817"
     CDS             9420..9833
                     /gene="pgaD"
                     /locus_tag="ACHJZ2_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO4566985.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(9883..10671)
                     /gene="phoH"
                     /locus_tag="ACHJZ2_000818"
     CDS             complement(9883..10671)
                     /gene="phoH"
                     /locus_tag="ACHJZ2_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO4566986.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(11291..12562)
                     /gene="efeB"
                     /locus_tag="ACHJZ2_000819"
     CDS             complement(11291..12562)
                     /gene="efeB"
                     /locus_tag="ACHJZ2_000819"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO4566987.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(12568..13695)
                     /gene="efeO"
                     /locus_tag="ACHJZ2_000820"
     CDS             complement(12568..13695)
                     /gene="efeO"
                     /locus_tag="ACHJZ2_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO4566988.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(13753..14583)
                     /gene="efeU"
                     /locus_tag="ACHJZ2_000821"
     CDS             complement(13753..14583)
                     /gene="efeU"
                     /locus_tag="ACHJZ2_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO4566989.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            15765..17108
                     /locus_tag="ACHJZ2_000822"
     CDS             15765..17108
                     /locus_tag="ACHJZ2_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566990.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(17257..18765)
                     /gene="putP"
                     /locus_tag="ACHJZ2_000823"
     CDS             complement(17257..18765)
                     /gene="putP"
                     /locus_tag="ACHJZ2_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO4566991.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(18924..19133)
                     /locus_tag="ACHJZ2_000824"
     CDS             complement(18924..19133)
                     /locus_tag="ACHJZ2_000824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4566992.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            19188..23150
                     /gene="putA"
                     /locus_tag="ACHJZ2_000825"
     CDS             19188..23150
                     /gene="putA"
                     /locus_tag="ACHJZ2_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4566993.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(23190..23828)
                     /gene="rutR"
                     /locus_tag="ACHJZ2_000826"
     CDS             complement(23190..23828)
                     /gene="rutR"
                     /locus_tag="ACHJZ2_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO4566994.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            24116..25207
                     /gene="rutA"
                     /locus_tag="ACHJZ2_000827"
     CDS             24116..25207
                     /gene="rutA"
                     /locus_tag="ACHJZ2_000827"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO4566995.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            25207..25899
                     /gene="rutB"
                     /locus_tag="ACHJZ2_000828"
     CDS             25207..25899
                     /gene="rutB"
                     /locus_tag="ACHJZ2_000828"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO4566996.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            25911..26297
                     /gene="rutC"
                     /locus_tag="ACHJZ2_000829"
     CDS             25911..26297
                     /gene="rutC"
                     /locus_tag="ACHJZ2_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO4566997.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINETYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            26305..27105
                     /gene="rutD"
                     /locus_tag="ACHJZ2_000830"
     CDS             26305..27105
                     /gene="rutD"
                     /locus_tag="ACHJZ2_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO4566998.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            27115..27705
                     /gene="rutE"
                     /locus_tag="ACHJZ2_000831"
     CDS             27115..27705
                     /gene="rutE"
                     /locus_tag="ACHJZ2_000831"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO4566999.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            27716..28210
                     /gene="rutF"
                     /locus_tag="ACHJZ2_000832"
     CDS             27716..28210
                     /gene="rutF"
                     /locus_tag="ACHJZ2_000832"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO4567000.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            28231..29559
                     /gene="rutG"
                     /locus_tag="ACHJZ2_000833"
     CDS             28231..29559
                     /gene="rutG"
                     /locus_tag="ACHJZ2_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO4567001.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(29642..29749)
                     /locus_tag="ACHJZ2_000834"
     CDS             complement(29642..29749)
                     /locus_tag="ACHJZ2_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFO4567002.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            30121..30717
                     /gene="wrbA"
                     /locus_tag="ACHJZ2_000835"
     CDS             30121..30717
                     /gene="wrbA"
                     /locus_tag="ACHJZ2_000835"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO4567003.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMS
                     PQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            30738..30965
                     /gene="yccJ"
                     /locus_tag="ACHJZ2_000836"
     CDS             30738..30965
                     /gene="yccJ"
                     /locus_tag="ACHJZ2_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO4567004.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(31003..32244)
                     /gene="agp"
                     /locus_tag="ACHJZ2_000837"
     CDS             complement(31003..32244)
                     /gene="agp"
                     /locus_tag="ACHJZ2_000837"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO4567005.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(32535..33792)
                     /locus_tag="ACHJZ2_000838"
                     /pseudo
     CDS             complement(32535..33792)
                     /locus_tag="ACHJZ2_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
     gene            34052..34972
                     /gene="cbpA"
                     /locus_tag="ACHJZ2_000839"
     CDS             34052..34972
                     /gene="cbpA"
                     /locus_tag="ACHJZ2_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO4567006.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            34972..35277
                     /gene="cbpM"
                     /locus_tag="ACHJZ2_000840"
     CDS             34972..35277
                     /gene="cbpM"
                     /locus_tag="ACHJZ2_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO4567007.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(35431..36030)
                     /gene="torD"
                     /locus_tag="ACHJZ2_000841"
     CDS             complement(35431..36030)
                     /gene="torD"
                     /locus_tag="ACHJZ2_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO4567008.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(36027..38573)
                     /gene="torA"
                     /locus_tag="ACHJZ2_000842"
     CDS             complement(36027..38573)
                     /gene="torA"
                     /locus_tag="ACHJZ2_000842"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO4567009.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(38573..39745)
                     /gene="torC"
                     /locus_tag="ACHJZ2_000843"
     CDS             complement(38573..39745)
                     /gene="torC"
                     /locus_tag="ACHJZ2_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO4567010.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            39875..40567
                     /gene="torR"
                     /locus_tag="ACHJZ2_000844"
     CDS             39875..40567
                     /gene="torR"
                     /locus_tag="ACHJZ2_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO4567011.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(40540..41568)
                     /gene="torT"
                     /locus_tag="ACHJZ2_000845"
     CDS             complement(40540..41568)
                     /gene="torT"
                     /locus_tag="ACHJZ2_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO4567012.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            41651..44395
                     /gene="torS"
                     /locus_tag="ACHJZ2_000846"
     CDS             41651..44395
                     /gene="torS"
                     /locus_tag="ACHJZ2_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO4567013.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            44467..45540
                     /gene="yccM"
                     /locus_tag="ACHJZ2_000847"
     CDS             44467..45540
                     /gene="yccM"
                     /locus_tag="ACHJZ2_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO4567014.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(45589..45723)
                     /locus_tag="ACHJZ2_000848"
     CDS             complement(45589..45723)
                     /locus_tag="ACHJZ2_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO4567015.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(45751..45981)
                     /gene="ymcE"
                     /locus_tag="ACHJZ2_000849"
     CDS             complement(45751..45981)
                     /gene="ymcE"
                     /locus_tag="ACHJZ2_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO4567016.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(45956..46144)
                     /gene="ymcF"
                     /locus_tag="ACHJZ2_000850"
     CDS             complement(45956..46144)
                     /gene="ymcF"
                     /locus_tag="ACHJZ2_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO4567017.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(46155..46367)
                     /gene="cspG"
                     /locus_tag="ACHJZ2_000851"
     CDS             complement(46155..46367)
                     /gene="cspG"
                     /locus_tag="ACHJZ2_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO4567018.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            46653..46865
                     /gene="cspH"
                     /locus_tag="ACHJZ2_000852"
     CDS             46653..46865
                     /gene="cspH"
                     /locus_tag="ACHJZ2_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO4567019.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            47307..47612
                     /locus_tag="ACHJZ2_000853"
     CDS             47307..47612
                     /locus_tag="ACHJZ2_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567020.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            47719..48363
                     /gene="gfcB"
                     /locus_tag="ACHJZ2_000854"
     CDS             47719..48363
                     /gene="gfcB"
                     /locus_tag="ACHJZ2_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO4567021.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            48360..49106
                     /gene="gfcC"
                     /locus_tag="ACHJZ2_000855"
     CDS             48360..49106
                     /gene="gfcC"
                     /locus_tag="ACHJZ2_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO4567022.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            49106..51202
                     /gene="gfcD"
                     /locus_tag="ACHJZ2_000856"
     CDS             49106..51202
                     /gene="gfcD"
                     /locus_tag="ACHJZ2_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4567023.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            51248..52387
                     /gene="gfcE"
                     /locus_tag="ACHJZ2_000857"
     CDS             51248..52387
                     /gene="gfcE"
                     /locus_tag="ACHJZ2_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO4567024.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            52375..52821
                     /gene="etp"
                     /locus_tag="ACHJZ2_000858"
                     /gene_synonym="yccY"
     CDS             52375..52821
                     /gene="etp"
                     /locus_tag="ACHJZ2_000858"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO4567025.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            52841..55021
                     /gene="etk"
                     /locus_tag="ACHJZ2_000859"
     CDS             52841..55021
                     /gene="etk"
                     /locus_tag="ACHJZ2_000859"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO4567026.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(55141..56445)
                     /gene="appA"
                     /locus_tag="ACHJZ2_000860"
     CDS             complement(55141..56445)
                     /gene="appA"
                     /locus_tag="ACHJZ2_000860"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO4567027.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(56525..56617)
                     /gene="cbdX"
                     /locus_tag="ACHJZ2_000861"
     CDS             complement(56525..56617)
                     /gene="cbdX"
                     /locus_tag="ACHJZ2_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO4567028.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(56630..57766)
                     /gene="appB"
                     /locus_tag="ACHJZ2_000862"
     CDS             complement(56630..57766)
                     /gene="appB"
                     /locus_tag="ACHJZ2_000862"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4567029.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(57778..59322)
                     /gene="appC"
                     /locus_tag="ACHJZ2_000863"
     CDS             complement(57778..59322)
                     /gene="appC"
                     /locus_tag="ACHJZ2_000863"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO4567030.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(59456..60313)
                     /gene="hyaF"
                     /locus_tag="ACHJZ2_000864"
     CDS             complement(59456..60313)
                     /gene="hyaF"
                     /locus_tag="ACHJZ2_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO4567031.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(60310..60708)
                     /gene="hyaE"
                     /locus_tag="ACHJZ2_000865"
     CDS             complement(60310..60708)
                     /gene="hyaE"
                     /locus_tag="ACHJZ2_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO4567032.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(60705..61292)
                     /gene="hyaD"
                     /locus_tag="ACHJZ2_000866"
     CDS             complement(60705..61292)
                     /gene="hyaD"
                     /locus_tag="ACHJZ2_000866"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO4567033.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(61289..61996)
                     /gene="hyaC"
                     /locus_tag="ACHJZ2_000867"
     CDS             complement(61289..61996)
                     /gene="hyaC"
                     /locus_tag="ACHJZ2_000867"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO4567034.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(62015..63808)
                     /gene="hyaB"
                     /locus_tag="ACHJZ2_000868"
     CDS             complement(62015..63808)
                     /gene="hyaB"
                     /locus_tag="ACHJZ2_000868"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO4567035.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(63805..64923)
                     /gene="hyaA"
                     /locus_tag="ACHJZ2_000869"
     CDS             complement(63805..64923)
                     /gene="hyaA"
                     /locus_tag="ACHJZ2_000869"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO4567036.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            65349..65436
                     /locus_tag="ACHJZ2_000870"
     tRNA            65349..65436
                     /locus_tag="ACHJZ2_000870"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:65383..65385,aa:Ser,seq:tga)
     gene            66039..66436
                     /gene="tccP2"
                     /locus_tag="ACHJZ2_000871"
                     /pseudo
     CDS             66039..66436
                     /gene="tccP2"
                     /locus_tag="ACHJZ2_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
BASE COUNT        16812 a        17053 c        16329 g        16242 t
ORIGIN      
        1 ctgtttacat accattgata aaactctgtc gctttcattg gttttgcata taagtatccc
       61 tgaactaatg tcacgttatt atgaataaga tattccgcct ggtgagaggt actgacacct
      121 tctgcaatcg ttatcaaatt gagtcgatta ctcagcttaa taataatatc taaaacaggc
      181 gtttcgtctt ccgctgtagt gagcgtgtct ataaagcttt tatctatttt aagataatca
      241 acagacaact tttgcagaag gccaagggaa caatatcccg tgccaaaatc atcaactgca
      301 aattcacagc catggctcct taattcattt attttctgta atgcatgatc agtatcttca
      361 accagattgc gttcggtaat ctcaaggaca atgttaaatt tatcagatat tgcatttttt
      421 atatgtaaaa catcagcaac aaaatcagga tgtgcaagat gttcagcggc aatatttatc
      481 ccaaggtgaa acggcttttt cactttccag ctttgtacat cctgaataat cagttcaaag
      541 agatgcttag ttaacggtat aattaaattt tcctcttctg cagcgcggat aaaaactacc
      601 ggagatatat ttttaccatc ttcgcgttgc caacgcatta acgcctcaac ccccagacac
      661 gctttggttg tcgtgtcaca tactggttga taatgcacag aaaattctcc ggatgacatt
      721 cctttcttaa tttcttctgc cagggacatt ttccgagata tccagcgctg ccataagaag
      781 tacaggataa aagaaagaag aatagataat ggaagaatgt aaaatacatt actaatgaca
      841 tagggatata atgaaatcat cggcgtagta actgtaagac tagcctgact gatcgcagaa
      901 ttaaaatctt tttttaacat atataacctg tcattattat ttacaccact aataattgca
      961 ggcccttcag taaagcgaag agaaaatgaa tcatcattct catcatcaag aacatccatc
     1021 agatcataga tatatcgtga ctctacaata ctaaaagctg tcatatcatt ccctattggc
     1081 ataaggaaca ctatggctgt tttacctggt acaaaacttg taccgctaat tgatactatg
     1141 cgtgattctt tatttttatt gtgaaagctc gtaccgtaaa catcgttaac attcattttt
     1201 ttgaaaccgc taactgaaga acatatttgg aaattatttt ctataagacc cgttgttcga
     1261 acataggggg tcaatgctcc gttagcaatc aattgttcca ggatatcttt gcagggtttc
     1321 atcaccagaa tagaagtaag ctttgtcgta tgtttgacaa tttgcgtaac attatcaagc
     1381 tgttttaaca gaataccggc ggtaattgtc gtatctcgat tcatttgaaa tactgacata
     1441 gcaaacgaca gtaacacccc cagaatgcct ggtattataa tgagtacaaa catttttttg
     1501 ttgaatacca tacatgtttc cccacgcatc agggtgatgc aacctaagaa attatagtac
     1561 aattgccagt aaaagcgggt gatagcagac tgatcaatga taagcattta agatcatata
     1621 gtactatgat atttctgaaa attgttacgc atgtctgcga atagctattt agcgcttacc
     1681 aaaatcaccc gctaaaatgc cgctttatgg tgactcacaa aaattcacca catccctcac
     1741 aaccacctta ttacgcccgg tctctttcgc ctcgtatagt gcgttatcag ccagtcgata
     1801 gatctcattc gggtttaacg catttccctg agtcacaaca gcgccaatac taatcgtcac
     1861 tttttgtggt atagcgtatt caggattatc gccagttaaa cgctcaacat tttcccgaat
     1921 cctttccgct aaagctttcg cacgttcagt atcgatgtcc gtcagcaaga cgccaaactc
     1981 ttcgccgccg acgcgcgcta aaaggtcatc tggtcgtatg cttttcccaa tgatattcac
     2041 cactgttttt atcacctgat cgccaaccgg atgcccccag gtgtcgttga cttttttgaa
     2101 gtggtcgata tccataatca tgacgcaata aggcgttttt ttggctgatg ctgattgaac
     2161 tgtcagttca ttaaaaaaat aatttctgtt aaatatattg gttaagggat cgcgatgtgc
     2221 aatgttcttt gttactcgta gcgcactgaa tatatggcac ataaatatta ccataacggt
     2281 taacttactg actacttcaa tagtgcgact aatataccaa gtggacacgc tatacctact
     2341 cataaataac agagagatat tgtacagtac ggcacaaaaa gcaatcaccg tcactccatt
     2401 ccagatatca tagcgtaaac cattaaagta tagattaacc gaaagcaagg ttaaccacat
     2461 gaaaatcatt atggtaacat atgaggcttt ccaaaccacc tgaccatttt cattggttaa
     2521 ctcggcaatg tgtaaattgt agctctcata atgactagaa agtatgtgag caactatcgc
     2581 ccccccaaaa acaaaaaaca aacttatgca caaagtcatt tttttggaaa ataaattgcg
     2641 ctgttttgtg ttttcactaa ctttattcac caatgccaga aatattaata tgcacaaact
     2701 catctgacga aacaaataat agattgcaat gtcattttgg acaacactgc ttgtcaaacg
     2761 cccctcaata atttgctgaa tgataatgac ggttttaaca aagtaaatca aacaactcag
     2821 aaaagccaaa ctaagtatac agtttgaaaa ttgcctacgg tcataaatga aatacaactg
     2881 cataaaaata aatgcaacag tatcgataaa aaataaagca atcattacca tcagataggt
     2941 tgatcgtgga acaacttcct caacgcctgg ctgattaaac caactattca ccaaaaccaa
     3001 agcaagccca aacaataagc tcactaatac agtactacta attctcaaat agtctttttc
     3061 cataaagcta caccaatctg taggttgtag atccctttct attattaata gatataagaa
     3121 ctgtactgtt gtcaatatga aattccgccc acaaacaata ccgatgatga tagttgctga
     3181 atgtaattat tattgcctaa gtgctttata cattgtttgc aacaagccag gcttgaaaat
     3241 acttataaat ggttatagaa aatccttata aaacattaat tcatagatac attaggaata
     3301 acaattaaat ccgtgagtgc cgtagcgcag cctttcatca ggactttcga aaaatccgaa
     3361 atcatgcatc ggaatttact gatttaatta ttttaatcct aatttatttt gaaaaaggca
     3421 tcgggattta tgccgtattc ctgaagatcc tcatcattgg aatggatttt cgggcgagaa
     3481 aaggatttta tatggacact ctgctcatca tttcttcttc tcatcatcaa caattcacgt
     3541 ctctcttccg cgtttaataa cggattatga ggtgcaaaaa tatctttctt ttcagttacc
     3601 tgtaattaga tacagagaga gattttggca atacatggag taatacagga tgtattcaag
     3661 tagcagaaaa aggtgcccga aaaccaaatg ggctttgaaa cttcttactg ccgcattttt
     3721 agcagcgagt cccgcggcga agagtgctgt taataacgcc tatgatgcat tgattattga
     3781 agctcgcaag ggtaatactc agccagcttt gtcatggttt gcactaaaat cagcactcag
     3841 caataaccaa attgctgact ggttacagat tgccttatgg gccgggcaag ataaacaggt
     3901 tattaccgtt tacaaccgct accgtcatca gcaattacca gcgcgtggtt atgcagctgt
     3961 cgccgtcgct tatcgtaacc tgcaacaatg gcaaaactcg cttacactgt ggcaaaaggc
     4021 gctctctctg gagccgcaaa ataaggatta tcaacgggga caaattttaa ccctggcaga
     4081 tgctggtcac tatgatactg cgctggttaa acttaagcag cttaactctg gagcaccgga
     4141 caaagccaat ttactcgcag aagcctatat ctataaactg gcggggcgtc atcaggatga
     4201 attacgggcg atgacagagt cattacctga aaatgcatct acgcaacaat atcccacaga
     4261 atacgtgcag gcattacgta ataatcaact tgctgccgcg attgacgatg ccaatttaac
     4321 gccagatatt cgcgctgata ttcatgccga actggtcaga ctgtcgttta tgcctacgcg
     4381 cagtgaaagt gaacgttatg ccattgccga tcgcgccctc gcccaatacg ctgcattaga
     4441 aattctgtgg cacgataacc cagaccgtac tgcccagtac cagcgtattc aggttgatca
     4501 tcttggcgcg ttattaactc gcgatcgtta taaagacgtt atttctcact atcagcgatt
     4561 aaaaaagacg gggcaaatta ttccgccctg ggggcaatat tgggttgcat cggcttatct
     4621 caaagatcat cagccgaaaa aagcacagtc aataatgacc gagctctttt atcacaagga
     4681 gaccattgcc ccggatttat ccgatgaaga acttgcggat ctcttttaca gccacctgga
     4741 gagtgaaaat tatccgggcg cgctaactgt cacccaacat accattaata cttcgccgcc
     4801 tttccttcgg ttaatgggca cgcctacgag catcccgaat gatacctggt tacaggggca
     4861 ttcgtttctc tcaaccgtag caaaatatag taatgatctt cctcaggctg aaatgacagc
     4921 cagagagctt gcttataacg caccaggaaa tcagggactg cgcattgatt acgcgagtgt
     4981 gttacaagcc cgcggttggc ctcgtgcagc agaaaatgaa ttaaaaaaag cagaagtgat
     5041 cgagccacgt aatattaatc tggaggttga acaagcctgg acagcattaa cgttacaaga
     5101 atggcagcag gcagctgtct taacgcacga tgttgtcgaa cgtgaaccgc aagatcccgg
     5161 cgttgtacga ttaaaacgtg cggttgatgt acataatctt gcagagcttc gtatcgctgg
     5221 ctcaacagga attgatgccg aaggcccgga tagtggtaaa catgatgtcg acttaaccac
     5281 catcgtttat tcaccaccgc tgaaggataa ctggcgcggt tttgctggat tcggttatgc
     5341 cgatggacaa tttagcgaag gaaaagggat tgttcgcgac tggcttgcgg gtgttgagtg
     5401 gcggtcacgt aatatctggc tcgaggcaga gtacgctgaa cgcgttttca atcatgagca
     5461 taaacccggc gcgcgcctgt ctggctggta tgattttaat gataactggc gtattggttc
     5521 gcaactggaa cgcctctctc accgcgttcc attacgggca atgaaaaatg gtgttacagg
     5581 caacagtgct caggcttatg ttcgctggta tcaaaatgag cggcgtaagt acggtgtctc
     5641 ctgggctttc actgattttt ccgacagtaa ccagcgtcat gaagtctcac ttgagggtca
     5701 ggaacgcatc tggtcttcac catatttgat tgtcgatttc ctacccagtc tgtattacga
     5761 acaaaataca gaacacgata ccccatacta caaccctata aaaacgttcg atattgttcc
     5821 ggcatttgag gcaagccatt tgttatggcg aagctatgaa aatagctggg agcaaatatt
     5881 cagcgcaggt gttggtgcct cctggcaaaa acattatggc acggatgtcg tcacccaact
     5941 cggctacggg caacgcatta gctggaatga cgtgattgat gctggcgcaa cgctacgctg
     6001 ggaaaaacga ccttatgacg gtgacagaga acacaactta tacgttgaat tcgatatgac
     6061 attcagattt taaggataaa tatgttacgt aatggaaata aatatctcct gatgctggtg
     6121 agtataatta tgctcaccgc gtgcattagc cagtcaagaa catcatttat accgccacag
     6181 gatcgcaaat ctttactcgc cgagcaaccg tggccgcata atggttttgt agcgatttca
     6241 tggcataacg ttgaagacga agctgccgac cagcgtttta tgtcagtgcg gacatcagca
     6301 ctgcgtgaac aatttgcctg gctgcgcgag aacggttatc aaccggtcag tattgctcaa
     6361 attcgtgaag cacatcgagg aggaaaaccg ctaccggaaa aagctgtagt gctgactttt
     6421 gatgacggct accagagttt ttatacccgc gtcttcccaa ttcttcaggc cttccagtgg
     6481 cctgctgtat gggcccccgt cggcagttgg gtcgatacgc cagcggataa acaagtaaaa
     6541 tttggcgatg agttggtcga tcgagaatat tttgccacgt ggcaacaagt gcgagaagtt
     6601 gcgcgttccc ggctcgttga gctcgcttct catacatgga attctcacta cggtattcag
     6661 gctaatgcca ccggcagctt attgcctgta tatgtaaatc gtgcatattt tactgaccac
     6721 gcacggtatg aaaccgcagc agaataccgg gaaagaattc gtctggatgc tgtaaaaatg
     6781 acggaatacc tgcgtacaaa ggttgaggta aatccacacg tttttatttg gccttatggc
     6841 gaagcgaatg gcatagcgat agaggaatta aaaaaactcg gttatgacat gttcttcacc
     6901 cttgaatcag gtttggcaaa tgcgtcgcaa ttggattcca ttccgcgggt attaatcgcc
     6961 aataatccct cattaaaaga gtttgcccag caaattatta ccgtacagga aaaatcacca
     7021 caacggataa tgcatatcga tcttgattac gtttatgacg aaaacctcca gcaaatggat
     7081 cgcaatattg atgtgctaat tcagcgggtg aaagatatgc aaatatcaac cgtgtatttg
     7141 caggcatttg ctgatcccga tggtgatggg ctggtcaaag aggtctggtt tccaaatcgt
     7201 ttgctaccaa tgaaagcaga tatttttagt cgggttgcct ggcaattacg tacccgctca
     7261 ggtgtaaaca tctatgcgtg gatgccggta ttaagctggg atttagatcc cacattaacg
     7321 cgagtaaaat acttaccaac aggggagaaa aaagcacaaa ttcatcctga acaatatcac
     7381 cgtctctctc ctttcgatga cagagtcaga gcacaagttg gcatgttata tgaagatctt
     7441 gccggacatg ctgcttttga tggcatattg ttccacgatg atgctttgct ttcagattat
     7501 gaagatgcca gtgcaccggc tatcacggct tatcagcaag caggctttag cgggagtctg
     7561 agcgaaattc gacaaaaccc ggagcaattt aaacagtggg cccgctttaa aagtcgtgcg
     7621 ttaactgact tcactttaga acttagtgcg cgcgtaaaag ccattcgcgg tccacatatt
     7681 aaaactgcac gaaatatttt tgcacttccg gtaatacaac ctgaaagtga agcctggttt
     7741 gcacagaatt atgctgattt cctaaaaagc tatgactgga ccgctattat ggctatgcct
     7801 tatctggaag gtgtcgcaga aaaatcggct gaccaatggt taatacaatt gaccaatcaa
     7861 attaaaaaca tccctcaggc taaagacaaa tctattttag aattacaggc acaaaactgg
     7921 cagaaaaatg gtcagcatca ggctatttct tcgcaacaac tcgctcactg gatgagccta
     7981 ttacaactga atggagtgaa aaactatggt tattatcccg acaattttct gcataaccaa
     8041 cctgaaatag accttattcg tcctgagttt tcaacagcct ggtatccgaa aaatgattaa
     8101 tcgcatcgta tcgtttttta tattatgtct ggtgttatgc atacccctat gcgtagcgta
     8161 ctttcactct ggtgaactga tgatgaggtt cgttttcttc tggccgtttt ttatgtccat
     8221 tatgtggatt gttggcggcg tctatttctg ggtctatcgt gaacgccgct ggccgtgggg
     8281 agaaaacgca ccagctcccc agttgaaaga taatccgtct atctccatta tcattccctg
     8341 ttttaatgag gagaaaaacg ttgaggaaac catacacgcc gctttagcac agcgttatga
     8401 gaacattgaa gttattgccg taaatgacgg ttcaacagat aaaacccgtg ccatcctgga
     8461 tcgcatggct gcacaaattc cccatttgcg ggtcattcat ctggcgcaaa accaggggaa
     8521 agccattgcg cttaaaaccg gagctgccgc ggcgaaaagt gaatatctgg tgtgcattga
     8581 tggcgatgcg ttattagacc gcgatgcggc ggcatatatt gtggaaccga tgttgtacaa
     8641 cccgcgtgtg ggtgccgtaa ccggtaatcc tcgtattcga acacgttcta ccctggtggg
     8701 taaaattcag gttggcgagt attcctcaat tattggtttg atcaagcgaa cccagcgtat
     8761 ctatggaaac gtatttaccg tttccggtgt tattgccgca tttcgtcgca gcgccctggc
     8821 agaagtgggt tactggagtg acgatatgat caccgaagat attgatatta gctggaagct
     8881 gcagttgaat cagtggacga ttttttacga gccacgggca ctgtgctgga tattaatgcc
     8941 tgaaacgtta aaagggctgt ggaaacagcg cctgcgctgg gctcagggcg gtgcagaagt
     9001 attcctcaaa aatatgacaa ggttgtggcg caaagaaaac tttcgaatgt ggccgctgtt
     9061 ttttgaatac agcctgacga caatatgggc cttcacctgc ctggtcggtt tcattattta
     9121 cgcagtccaa cttgccggtg taccgttaaa tattgaattg acacatatcg ctgcgacaca
     9181 tactgccgga atattattgt gtacgttatg tttactgcaa tttattgtca gcctgatgat
     9241 cgagaatcgc tatgagcata atctgacttc atcgcttttc tggattattt ggttcccggt
     9301 tattttctgg atgctgagcc tggcaacaac attggtatca tttacacgag tcatgttgat
     9361 gcctaaaaag caacgcgccc gttgggtaag tcccgatcgc gggattctga gaggttaata
     9421 tgaacaattt aattattacg acccgacaat caccagtacg tttactggtt gattatgttg
     9481 ccacaaccat cttgtggaca ttatttgcgt tgttcatatt cttattcgcc atggatctgc
     9541 tgacgggtta ttactggcaa agcgaggcca gaagccgact tcagttctat tttttgctgg
     9601 cagtggcgaa tgccgtcgtg ttaattgtct gggcgctgta caataagctg cgttttcaaa
     9661 aacagcagca tcatgcagcc taccaatata cgccgcaaga atatgcagag agcttagcaa
     9721 tacctgatga gctctatcag caactacaaa aaagccacag gatgagcgta cacttcacca
     9781 gccaggggca aataaaaatg gttgtttcag aaaaagcgct agtcagggca taaacaccca
     9841 aaacaaagcc cggttcgccc gggctctgca ccgataatac acttaactgt atgcatgcag
     9901 cgtacgttgg caaagtgccg aacgtacgca atcctctttt ccgaaccgga cgatcccaac
     9961 catttcatct tcttcgaaac gttccagcgc gtcacttaat ccggagcaca cgccgcgagg
    10021 caaatcgcat tgcgtgatat caccgttgac gataaccgtc acgttctccc cgaggcgggt
    10081 taaaaacatt ttcatttgcg cggcagtcac attctgcgcc tcgtcaagaa tgacgactgc
    10141 attttcaaag gtacgtccac gcatataggc gaacggcgca atttccacct tccctatttc
    10201 cggtcgcagg cagtactgca taaaggaagc ccctaagcgc cggaccagca cgtcgtagac
    10261 cgggcgaaaa tagggagcaa acttttctgc gatatctcca ggtaagaagc caagatcttc
    10321 atcggcttgc agaactggac gggtgacgat aatcctgtcg acatccttat gtatcagggc
    10381 ctctgccgct tttgctgcgc tgatccaggt ttttccgcac ccggcttcgc ccgtggcgaa
    10441 tatcagctgc ttactctcaa tagccttcag atagtgcaat tgcgcttcat ttcgcgcgag
    10501 gatgggcgaa gtatcgcgac tgtcgcgggc cataccaatg gcttctacgc cgcccatctg
    10561 cacaagcgag gtgaccgatt cttcttcacg ctgcttatgg ctgcgcgaat cccgtctcag
    10621 cacacgtttt gcctcgcgac gagctttgat cactgctttt tgtcttccca tggagagcac
    10681 cttgagttgt ttgtattcat cacacgcgcc gttggcagcg cgattatgcg cacgaacatc
    10741 agagggttgg cttccttgta agccatagtt tgcttttgga taaaatgccg aaaaacggct
    10801 acgcgcaccg tttacggcgt cggtaacaca tgaaaagaaa ggatgaggtt gaaaatgcaa
    10861 agtgacgaga tgactaccgg aggagaaaac tccgcgagtg gtggcgcgtt gattatctaa
    10921 aacatgtcca gtacaggacg ttaccatccg cgatctccat agtgactgac tatcactgcc
    10981 gggaacttcc gctgctactt aataagtaca acagttctcg catttattgc aacaatatat
    11041 ttacttatat ttaactataa aacaccattt cagtgacatt agtttctact ggaaagatga
    11101 cagagtgatg acagatatga aaaaagctgt gtgctttcag caggatttgc atcaggcatc
    11161 agtgcacgat cgccgtatgc ggcgtgaacg ccttatccgc ctaccgaacc cgcaccgatt
    11221 gtaggcataa gaagacgcgc aagcatcgca tcaggcatct gcgcaagaac gccttatccg
    11281 tctaaaaaca ttaaacccgc aataacgcgc ttccgagata atcgttcgcg tccttcactc
    11341 ccggcagcgc aaaaaaataa ccgccgccga taggtttaac gtattcctcc agcgcttcgc
    11401 cattgagcct tttttgtact gtcaggaagc ctttttccag atcgtgttgg taacagacaa
    11461 acagtagccc catatccagt tgcccggagt tggtgacgcc cagtgaataa ctgtagccac
    11521 gacgcagcat caggctggac tcactctccg ccgtgcgggg attcgccagc cggatatggc
    11581 tgtccagcgc aatccccttc ccttccgggt cgctggcgta atcaggcaca tcatgctcat
    11641 gctgcattcc cagcggcgca ccggtttgct tatcacggcc aaaaatcgtc tgctgttctt
    11701 tcagcggcgt tctgtcccaa aattccactc gaaactgaat caagcgtact gcctgatagc
    11761 tgccaccgat tgtccacgca ggctcctgct gatctgccgt tacccacacc actttttgca
    11821 tcaacttatc attctggcta tcgggattgg cagtgccgtc tttgaaaccc agcaaattaa
    11881 tcggcgtctc tttgccttta ctacgcgccg cgtgatcgga aataaaccct tcccgcttcc
    11941 agcgcacgct gagcaaatcc ggcgtatgtt tgatgatatc gcgcagtgca tggatcaccg
    12001 tgtcctgggt gttggcgcaa atttgtagca acacatcacc atgacataac gccgcatcca
    12061 gcgagtcgtt gggaaaacgc gtcatcttct gcaacttttt cggcatctgt ggcgcaaggc
    12121 caaagcgctc atcaaacaat gagtgaccca ccgataacgt gatggtgaga ttatcgggcg
    12181 caatgtagcc gccaagaatg ccggaatcga gtggtggcag gcgcggattt ggcgtttctg
    12241 gtgccgctcc gccctgagtc agaaaagcaa aacgctgagt caacaagcga aacaaccgtt
    12301 caagatcggc tttatcgctg gcaagcacat caaacgccac cagcatcatt gcggcctgtt
    12361 gtggcgtcag gatccctgcc tgatgctcac cataaaacgg ctgtttctca ttgcgtgcaa
    12421 ccggtgaaag cgtacccggc gcactttgcg ttttttgtgc atgagcgacc ggacaacttc
    12481 ccgccagcgc cagcgcacca atgcctttca gtaaacgtcg gcgtgacggt tcattcacgc
    12541 cgtttttatc ttcatactgc ataatgctta atccaatccc agcacaccgc gaagttgcgc
    12601 cagatcttcc gccagcgcag taatcggtcc tttcagtgca ttccggtcgg catcggtcaa
    12661 tttgtcgtag gtttcaaaac cgtctttagt acggtatttc gccagaatgg tatcgacctt
    12721 tttaaagttg gcatcgactt ttgccaacag ttccgggtta gctttttgca gttgtggacg
    12781 cagcagatcg acaattttct gcgagccttc aacgttagcc tggaaatccc acagatcggt
    12841 gtggctgtag cgatcttctt caccgctgat tttgctggct gccacttcct caatcagtcc
    12901 ggctgcaccg ccgaccactt ttgaaggtgg gaaagccagt tcactgatgc gtttttgcaa
    12961 atcgaccaca tcggtataaa gctggtcagc gtactgatcc atgcctttgg tggtgttgtc
    13021 gccaaacaat gctttttcca gacggtggaa accggtgaat tttggatcag cggctttttg
    13081 ctcgtaatca tcttcacggg cgtcaatgct gccatccaga tcggagaaca gttcagcaat
    13141 cggttcaatg cgctcatagt gctggcgcgt cggcgcatac agtgctttcg ctttttcgat
    13201 atcgcctgct ttaatcgcgt cggtaaaggc tttggtgtcg gtcaccagct gtgtggtttc
    13261 cgccatgaca tacgctttat atgcagtaat tgcaccacca agacttaaca gcgcatcact
    13321 ttgcgccgca tccgccgttg cctcaccttt gacgatcaac ttccctttcg ggttagtcag
    13381 cagaccgcag gtcatatcgt attcgccagg ctgtaaattc gccgtcattt tctggctaaa
    13441 gccaggggcg atattttccc gctcttccac caccatcacg cctttgagga tctcccattc
    13501 cagcgccttc tggctgtggt tctgaataat gaactgtgtt ttcccggcgt taaccgtaat
    13561 ggtcatcggt tcgcactgct tatccgtcac ggtcactttg acctgcggca catcagcggc
    13621 gttagccata aaagcagaag aaaacagcgc agccacgctc aactgcaatg cgttacggcg
    13681 gaagttaatg gtcatgacga gactatccct ttaaagtatg ttgtaactaa gcaagagttt
    13741 gcgtcgtatt tgttacgcgg agcgagacgc tgtcgcccct gcgcgtggtg gcagagcaaa
    13801 tgccaccagc gccgggatga gataaataaa ccagacggcg acttcgctga cgctcggcgc
    13861 ttcctgatag ccaaaaatcc cttccatcag cgtgccaaac agcgagtgag ttgagagcac
    13921 cgcactcata tcgaaggcga tttcctgaaa gtggttccac aatccggctt catgaaatgc
    13981 gcgaatggca ccagctgcca gccctgcggc gacgaagaga ataaacaggc tggtccattt
    14041 aaaaaatgca ccaagattga ggcgaatacc gccccagtag agcaggaagc ctagcaccac
    14101 ggcagtagca agaccgagca ttgcacccag cggcggccag atcccgacat cttgttgaaa
    14161 tgccgccagc aggaaaaaga ccgactccag cccttccctt gcaacggcaa aaaagaccat
    14221 catcaccagc gcccagccat gatgatttcc acgctgcaat gcgctatcga cagcctgctc
    14281 cagttgtact ttgacgttgc gcgacacttt gcgcatccag aaaaccatcc aggtaaggat
    14341 caccacggcg atcaccgcca cgataccttc aaacagttcc tgctcttttt gcggaaattc
    14401 gccggtggtt tcgttaatga agatccccaa gcccaggcac aacgcagcgg caagcaacac
    14461 gccaatccac atcacaccaa tccatcggcc tcgctgggta cgcttaagat agctggcaat
    14521 caaactgaca atcagcgcgg cttcaagtcc ttcgcgcaac ataatgagaa acggaacaaa
    14581 catgccaccc acccttaaac gtttttctga gtcaatctat gcaaagaaaa gtaaatcgca
    14641 gtgatagcga ttatcattac gctaaagaaa aatacaagcg gaatatttat aaaatgatgt
    14701 tggaatgtaa aaatgaggag atcaaatccg gagaaaaaag cgattatttt aaggtattta
    14761 gctgacgttt cgcccggcag atgaagcttg tacgaatgtt ggtggcgaca aactcgctgc
    14821 agcgtgcccg aagggttgaa ttttcagtta gtcgtttcac aaaagaacgt tgttttttgc
    14881 ttattaatag atagtgataa ggttgtgaat gaaattatac ctcatctcct catcgaattt
    14941 tttgcccaca acctaaacag gagaaaacgt gtatttaagt ctggaatgag cgatttttga
    15001 tagtgcctaa ttatccccca aaattataat tccccttatt atattcccag tgatatcatt
    15061 ttatggctaa tgctacatac aattctaaat cacgataaaa cgaagcagga tgcgcattaa
    15121 taaagtcaca cataataaaa atagaaatct taaccagaag taatagatca tcgtgaaaat
    15181 caatcaccaa aaacaccaac acacaaaaat caataattta ataattgttc agggcttttt
    15241 tctcacgatt tttctactat gacattatca caaacccact agcatgaata cagaaagaga
    15301 agtaataaga tgagacgcca ctgctttcag atgaaccctg taattagggt cgagaatata
    15361 atttacccat tcccctaacg ataaattaaa aaatttgcga gttaattttt ctctggctga
    15421 aggctaacgt cgagttatcg ataaaattca cgagtaattg taactatata ctttatgaaa
    15481 cacaggaaga aattattatt taacgcgatg ataaaaggcg taaaaaaaaa taataatctt
    15541 catgaaactc accccgatgg cagcattgta aattcgccaa taactggcat tcctcctgct
    15601 ttttacgggt gtttccatta acaccagaat gagcggattg tttactacat caagaacgca
    15661 tccgaagtgt acatcctggt gcgccgactg aatcgcccgg agccatgcgg atggctacga
    15721 cttaatacga atcattaacc ctgtaaggat acggagagca ttacatgaga ccaatatcca
    15781 acctcaatac gagcgcagca acctatattc caccgcaaca actcccgagt agttgtctgg
    15841 aaactttgac gcttctggtg cagatgaata attatatcaa aagggatgcg gactacagca
    15901 cgggcatggc tgtagctcca ctggttcctg aagaggttca cttgctggct gctgcgatga
    15961 caaccgaact gcatcgacat cagttccaac cggtggtaag tgcaaatgat ctgcaacttc
    16021 ctgaaccgtt caccttcgat gttgccggtt tcaccatcac cttcacgaag acgcaagagc
    16081 atgacaacac gggcaatctc atgaaaattg cagtcagcaa gagcggtgtg tgcaccagta
    16141 caaatattac tcttgagctg tttcactcca ttgtcacaac cctgatgtca cgcagtcaat
    16201 atggaacctt tgatctctgg tccgtacggc caatactcac ggatgaaagt caaaacaggg
    16261 tgcatgaagc agctcgctac tccccggccc agcaatacgg aagagaagaa acacacttta
    16321 ccaacaatgg catgcgagaa tttggaaata tgtctccgct ggcgtggcgt aacgatgtgg
    16381 aattacagca atcgtgtaat acaagcaaca tcccaccaaa tgctgcaaat gacgacataa
    16441 acaacaccag gcagaccatc gaggaccagc cagaggcgga cgagccacag caatatgtta
    16501 aattgactgt agacgacatg cgaaaatggg cggctatgga ccagcaggcg cgaaacgctc
    16561 tacagggagt ttcaggatgg tgtacgcgca atcattttaa tatcaaaaat gccagaaatt
    16621 acctgaccga tcacggcctg aactacgcgg gacaggtaaa ggtaaacagg ccgcatgaat
    16681 atgccaaatt cacccttgaa catatccgcc agtgggcagc cttaaacaag catgtgcgaa
    16741 aaccggttgg ttatttagaa aagtggtgta aggaaagaaa cctcgcgcca actactgcca
    16801 ggaattacct gaagaacgac gggcttaccg ccctgggtga gttgaagctg acagggccgc
    16861 agaaatgggt cacgttcacc ttcggagaca tcgtacaatg ggcgaatatg acccaggagg
    16921 agcgtaacag cgcaggggga gccgaaaagt ggagtaaaaa acgcggcttc cagtggtcta
    16981 ctgccagatc ctacctgaaa tcgagcggag tgaccaagca aggtgcgaga aaactggcgt
    17041 ggttaaaaaa cagcgggaat atgtcgaatc cattctatct cgcccaacca accagccgca
    17101 ggacataagc tgaaggtacc gatagcggca attttgttca tctgtgccgg gtgggcaaat
    17161 agcaattgcc cccgaacact ccctgccagg caacttatac cgcatggcag ggaacattcg
    17221 ggttaaaaag caaaagataa ctaattactc ttcagcttag ccttcctgca accgtgacgg
    17281 cggagccgaa tgatagtgcg catcggcctc ggcaaagcgt ttttgcatcg ccgctgacgg
    17341 tgctttaccc agcaaactaa acactacaat cccaatactg ccaaagataa agcccggaat
    17401 aatttcgtac agtcccagcc agccgaactg tttccagacg atcaccgtca gcgcaccgat
    17461 gatcatcccc gccagcgcac cgttgcgcgt catgcgtgac cacatcaccg aaaacagcac
    17521 caccggacca aacgccgcgc caaagcctgc ccacgcgtag ctcactaagc ccagcacgcg
    17581 gttttccggg ttcgctgcca acgcaatcgc caccagcgcc accaccagca ccatcacacg
    17641 ccctacccac accagttctt tctggctggc atgtttacgc agaaacgctt tgtacaaatc
    17701 ttcggtaatc gcactggaac ataccagcag ctggcaactt aacgttgaca ttaccgccgc
    17761 cagaatcgcc gacagcagaa tcccggcaat ccacgggtta aacagaattt gcgccagttc
    17821 gataaacacg cgctcggcgt tctggtttac cgcaccagct accgacgggt gctcgttaaa
    17881 gtaagcgata ccaaagaagc cgacagccac cgcccctgcc aggcagagga tcatccaggt
    17941 catactgata cgacgcgcat ggacgatgct gtggtgagaa tccgccgcca taaaacgcgc
    18001 caggatgtgc ggttgcccga agtaacccag cccccacccc atcagcgaga taatggcgac
    18061 aaagttcagc cctttgagca tatccacgtt ttcgatgctc ttttgtttga tcacttccag
    18121 cgagtcacca aagccaccga cactgataat gacgataacc ggcgtcagga tcagggcaaa
    18181 aatcatcagg ctggcctgta cagtgtcagt ccagctcacc gcgaggaaac cgccaataaa
    18241 ggtgtaaagg atcgtcgccg cagctcccgc ccacagagcc gtttcgtagc tcatgccaaa
    18301 ggtactttca aacagacgcg cgcctgccac aatgcccgaa gcgcaataaa tggtgaagaa
    18361 cagcaaaatc accagcgcgg agataatgcg caaaatgcgg cttttatctt caaaacgccc
    18421 ggtgaaataa tccggtaatg ttaaggcgtt attgttgtat tcggtatgca cacgcaaccg
    18481 cccggccacc agcttccagt taatccacgc gcctaatgtc aggccaatgg cgatccaact
    18541 ttcggaaatc ccggaaagaa aaaccgcgcc cggcaacccc attaacagcc agccgctcat
    18601 atccgatgca cccgccgata atgccgtcac gaatggccca aggctacgtc cgcccagaat
    18661 atagtcgtca aagtttttcg ttgatcgcca ggcgataaac ccaatcaata tcatgccaaa
    18721 gatatagaca caaaatgtca ccaacatcgg tgtgctaata gccatctaaa gtctccaaaa
    18781 aattattatc ggcaatgtcg aaacttgccg ttatatctgc caccggaacg gggtaacaga
    18841 gtttatgttt taccagggcg accgtatcct gccggaagcg ctggttattc acaatcgatt
    18901 taacacacca tttacattaa attttagtgc tcagcgacac tatttttcat caggttgcac
    18961 tctctcacat tttttgcggt tgcacctttc aaaaatgtta actgccgcag agaaaaagtc
    19021 tgagttattt ttttaatccc tgtcatatcg atttctttta ttaacatttc attcattttt
    19081 aagcttgcta cgcaggtcac atttaacatg gttgcacaaa gttgcaacat catggatatt
    19141 tcacgataac gttaagttgc acctttcaga acaacaggag taatggcatg ggaaccacca
    19201 ccatgggggt taagctggac gacgcgacgc gtgagcgtat taagtctgcc gcgacacgta
    19261 tcgatcgcac gccacactgg ttaattaagc aggcgatttt ttcttatctc gaacaactgg
    19321 aaaacagcga tactctgcca gagctacctg cgctgctttc tggcgctgcc aatgagagcg
    19381 atgaagcacc gactccggca gaggaaccac accagccatt cctcgacttt gccgagcaaa
    19441 ttttgcccca gtcggtttcc cgcgctgcga tcaccgcggc ctatcgccgc ccggaaaccg
    19501 aagcggtttc tatgctgctg gaacaagccc gcctgccgca accagttgct gaacaggcac
    19561 acaaactggc gtatcaactg gccgataaac tgcgtaatca aaaaaatgcc agtggccgcg
    19621 caggtatggt ccaggggtta ttgcaggagt tttcgctgtc atcgcaggaa ggcgtggcgc
    19681 tgatgtgtct ggcagaagcg ttgttgcgta ttcccgacaa agccacccgt gatgcgttaa
    19741 ttcgcgacaa aatcagcaac ggtaactggc agtcacacat tggtcgtagc ccgtcactgt
    19801 ttgttaatgc cgctacctgg gggctgttgt ttactggcaa actggtttcc acccataacg
    19861 aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa gagcggtgaa ccgctgatcc
    19921 gcaaaggtgt ggatatggca atgcgcctga tgggcgagca gttcgtcacg ggcgaaacca
    19981 tcgcggaagc gttagccaat gcccgcaagc tggaagagaa aggtttccgt tactcttacg
    20041 atatgctcgg cgaagctgca ctgactgccg cagacgcaca ggcgtacatg gtttcctatc
    20101 agcaggcgat tcacgccatt ggtaaagcgt ctaacggtcg tggcatctat gaaggtccgg
    20161 gcatttcaat caagctgtcg gcgctgcatc cgcgttacag ccgcgcccag tatgaccggg
    20221 taatggaaga gctttacccg cgtctgaaat cactcaccct gctggcgcgt cagtacgata
    20281 ttggtatcaa cattgacgcc gaagaggccg atcgtctgga gatctccctc gatctgctgg
    20341 aaaaactctg tttcgagcca gagctggcag gctggaacgg catcggtttt gttattcagg
    20401 cttatcaaaa acgctgcccg ctggtgattg attacctgat tgatctcgcc acccgcagcc
    20461 gtcgccgtct tatgattcgc ctggtgaaag gcgcgtactg ggatagtgaa atcaagcgcg
    20521 cgcagatgga cggccttgaa ggttatccgg tttatacccg caaggtatat accgacgttt
    20581 cttatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa tctaatctac ccgcagttcg
    20641 caacgcacaa cgcccacacg ctggcggcga tttatcaact ggcaggtcag aactactatc
    20701 cgggtcagta cgagttccag tgcctgcatg gtatgggcga gccgctgtat gagcaggtca
    20761 ccgggaaagt tgccgacggc aaacttaacc gtccgtgtcg tatttatgcc ccggtcggca
    20821 cacatgaaac gctgctggcg tatctggtgc gccgcctgct ggaaaacggt gctaacacct
    20881 cgtttgttaa ccgtattgcc gatacctctt tgccactgga tgaactggtc gccgatccgg
    20941 tcactgctgt agaaaaactg gcacaacagg aagggcaaac tggattaccg catccgaaaa
    21001 ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa ctcggcaggg ctggatctcg
    21061 ctaacgaaca ccgtctggca tccctctctt ctgccctgct caatagtgca ctgcaaaaat
    21121 ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg tgagatgtcg cccgttatta
    21181 acccggcgga accgaaagat attgtgggct atgtacgtga agccacgccg cgtgaagtag
    21241 agcaggcgct ggaaagtgcg gttaataacg cgccaatctg gtttgccact cctccggctg
    21301 aacgcgcggc gattttgcat cgcgctgccg tgctgatgga aagccagatg cagcaactga
    21361 ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa cgccattgcc gaagtgcgtg
    21421 aagcggtcga ttttctccac tactacgccg gacaggtgcg ggatgatttc gctaacgaaa
    21481 cccatcgtcc attagggcct gtggtatgta tcagcccgtg gaacttcccg ctggcgattt
    21541 tcaccgggca gatcgctgcc gcactggcgg caggtaacag cgtactggca aaaccggcag
    21601 aacaaacgcc actgattgcc gcgcaaggga tcgccatttt gctggaagcg ggcgtaccgc
    21661 caggcgtggt gcaattgctg ccaggtcggg gtgaaaccgt gggcgcgcaa ctgacgggtg
    21721 atgatcgcgt acgcggggtg atgtttaccg gttcaaccga agtcgctacg ttactgcagc
    21781 gcaatatcgc cagccgcctc gacgctcagg gtcgccctat tccgctcatc gctgaaaccg
    21841 gcggcatgaa cgcgatgatt gtcgattctt cagcactgac cgaacaagtc gtaatagatg
    21901 tgctggcctc ggcgttcgac agtgcgggtc agcgttgttc ggcgctgcgc gtgctgtgcc
    21961 tgcaagatga gattgccgac cacacgttga aaatgctgcg cggcgcaatg gccgaatgcc
    22021 ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc ggtgattgat agcgaagcga
    22081 aagccaatat tgagcgccat attcagacca tgcgtagcaa aggccgtccg gtgttccagg
    22141 cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag cggcaccttt gtcgccccga
    22201 cgctgatcga actggatgac tttgccgaat tgcaaaaaga ggtctttggt ccggtgctgc
    22261 atgtggtgcg ttataaccgt aatcagctgc cggagctgat cgagcagatt aacgcttccg
    22321 gctatggtct gacgcttggc gttcatacgc gtattgatga aaccatcgcc caggtcactg
    22381 gctcagccca tgttggtaac ctgtatgtta accgtaatat ggtgggcgca gtggttgggg
    22441 tgcagccatt cggcggcgaa gggttgtccg gtaccgggcc gaaagcaggc ggtccgctct
    22501 atctctaccg tctgctggcg aatcgcccgg aaagtgcgct ggcggtgaca ctcgcgcgtc
    22561 aggatgcaga gtatccggtc gatgcgcagt tgaaagccgc attgactcag ccgctaaatg
    22621 cactgcggga atgggcagca aatcgtccag aattgcaggc gttatgtacg caatatggcg
    22681 agctggcgca ggcaggaaca caacgattgc tgccagggcc gacgggtgag cgcaacacct
    22741 ggacgctgct gccgcgtgag cgcgtattgt gtatcgcgga tgatgaacag gatgcgctga
    22801 ctcagctcgc cgccgtgctg gcggtgggca gccaggtgct gtggccagat gacgcgctgc
    22861 atcgtcagtt agtgaaggca ttgccatcga ctgtcagcga gcgtattcaa ctggcgaaag
    22921 cggaaaatat taccgctcaa ccgtttgatg cggtgatctt ccacggtgat tcggatcagc
    22981 ttcgcgcatt gtgtgaagca gttgccgcgc gggatggcgc aattgtttcg gtgcagggtt
    23041 ttgcccgtgg cgaaagcaat atcctgctgg aacggctgta tatcgagcgt tcgctgagtg
    23101 tgaataccgc tgccgctggc ggtaacgcca gcttaatgac gatcggttaa acaggtagcc
    23161 ggaggatgtt acaccctcct ccggcatcat tagcgtggtc gaatcccctc aataataatc
    23221 cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt tgcgcctgtc
    23281 accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca aatcataaaa
    23341 atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc ggcaatcagc
    23401 gccgatttct catcaattaa tgacttcaaa tcgcccgtca gttcatccat taacagcggc
    23461 gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc gcgtgagact
    23521 tccagcttca gacggatgta ctctttgatc gccgccagcg gggcgaaatc ttcacgaaac
    23581 gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat atacagcgcc
    23641 tcttttgacg gaaagtaata cagcagattg gtttttgaaa cacccgccaa ctctgcaatt
    23701 tgctccagcc gtgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc tgcgctaaga
    23761 atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt cactgcgcct
    23821 tgcgtcatgc gctctcccct cttgtagatt ggcaacagga tagcaaaacc cccgcccggc
    23881 ctcgaccttc tgcactctca tcgcgcacag atgcatgttt tatgtgcaac tgttttgacc
    23941 gtttagtcca ctttttacca gacattttag ataacgcgat gttaacacat taataacact
    24001 ccctttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg aaaaggatat
    24061 gcagaatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt tgatgatgaa
    24121 aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc acgcgccgca
    24181 gtacatgccg acctttgaac tgaataaagc catcgtgcaa aaagcggagc actaccattt
    24241 cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg agttctggga
    24301 tcacaacctt gagtcgttca ccttgatggc ggggctggcg gccgtgacct cgcgcattca
    24361 gatttacgcc accgccgcca ccttaacgtt acctccggca atcgtcgccc gtatggccgc
    24421 aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg gctggcaaaa
    24481 gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc gtcgttacga
    24541 ctatctcacc gagtatgttc aggtgctgcg cgacctgtgg ggcacgggaa aaagcgattt
    24601 taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga gtgtccccat
    24661 gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgctc agtatgccga
    24721 tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc cgaccgctgc
    24781 gcggatgaaa caggccgcag agcaaaccgg gcgcgacgtt ggctcttatg tattgtttat
    24841 ggtgattgcc gatgaaaccg acgatgccgc tcgcgccaaa tgggaacact acaaagcggg
    24901 cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata cccgctccgg
    24961 tactgacacc aacgtccgtc agatggccga tcccacttcg gcggtaaaca tcaatatggg
    25021 gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg caagcgtgcc
    25081 tggtgccgaa ggcgtgctgt taaccttcga cgattttctg tcgggaatcg aaaccttcgg
    25141 cgagcgcatt caaccactga tgcagtgccg cgcccatctc cctgtgctga ctcaggaggt
    25201 ggcatgatga cgacattaac cgctcgaccg gaagccatta ccttcgatcc gcagcaaagt
    25261 gcgctgatcg tggtggatat gcaaaacgcc tatgccacgc caggcggcta cttagatctc
    25321 gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac cgccgtgacc
    25381 gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga tgaacagtat
    25441 gtcgaggctg gcggccctgg ctcaccgaat tttcataaat cgaacgccct caaaaccatg
    25501 cgtaaacagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga ttatcaactg
    25561 gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg ctacagcggt
    25621 ttcttcaata cgccgctgga cagcattttg cgcagccgcg gaatacgcca tctggttttc
    25681 accagcatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt ttttctggag
    25741 tatttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt tgtacagaaa
    25801 gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga aacgttctgc
    25861 gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttaacg atgccaaaat
    25921 ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc ggcacgctgg
    25981 ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacacaat aacgtgctgt
    26041 ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc aaggtaatcg
    26101 agacagcggg tggcacgatg gcggatgtga ccttcaacag catctttatt accgactgga
    26161 aaaattacgc cgcgattaac gaaacctacg ccgagttttt tccaggtgat aaaccggcgc
    26221 gattctgcat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc gccacaattg
    26281 cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc ttatgctgat
    26341 gcgcccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg gttaccgcaa
    26401 ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg caccggcaat
    26461 aatcccgaca cgctggcaga agattacagt atcacccaga tggcagcgga actgcatcag
    26521 gcgctggtag ccgcagggat tgagcattac gcagtggtcg gccatgcgct cggtgcgctg
    26581 gtgggaatgc agctggcgct ggatcatccc gcgtcggtaa ctgtgctggt ctgcgttaac
    26641 ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga acgattactg
    26701 tatagcggtg gcgcgcaggc atgggtggaa gcgcagccgt tgttcctcta tcccgccgac
    26761 tggatggcgg cccgcgcacc tcgccttgag gcagaagacg cgctggcact ggcgcatttt
    26821 cagggcaaaa ataatttact gcgtcgactt aacgccctca aacgcgctga ctttagtcac
    26881 catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga tctgctggtg
    26941 ccatcagcat gttccagtga acttcatgcc gccctgcccg atagccagaa aatggtgatg
    27001 cgctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc tctgttactc
    27061 aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat tgctatgaac
    27121 gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac tcacaacggc
    27181 tggcgggaga cacccgtcag cgatgagacg ttacgggagc tttatgccct gatgaaatgg
    27241 gggccgacat cagctaactg ttctccggca cgtatcgtgt ttatccgcac tgcagaggga
    27301 aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct gaccgcgccc
    27361 gtcaccgcta tcgtcgcatg ggacagtgaa ttttatgaac ggttaccgca actgtttcct
    27421 cacggcgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga aacagcgttt
    27481 cgcaacagtt ccatgcaggc ggcttatctg atcatcgcct gccgtgcgct ggggctggat
    27541 accggcccga tgtcgggttt tgatcgtcaa cacgtagacg acgctttttt tgcaggcagc
    27601 acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa actttttgcg
    27661 cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc aaactatgaa
    27721 cattgtcgat caacaaactt ttcgcgatgc gatgtcctgc atgggcgcgg cggtcaatat
    27781 catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg tctgcagcgt
    27841 gaccgacacg cctcccacat tactggtgtg cctgaatcgt ggggcgtccg tctggccggt
    27901 attcaatgaa aatcgaacgc tgtgtgtaaa tacacttagc gccgggcagg agccgctttc
    27961 aaaccttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc gctggcagac
    28021 tggtgtgacc ggatgcccgc aactggaaga ggcgctggtt tcgtttgact gccgtatcag
    28081 ccaggtggtg agcgtcggca cccacgacat tttgttttgc gccatcgaag cgattcatcg
    28141 tcacgccacc ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc tgatgcgccc
    28201 tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggtcttcc tcactggcag
    28261 ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt gccgtttgct
    28321 cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac ggtgttaatg
    28381 ccgattctga tggggctgga tcccaatctt tccattttaa tgtcagggat cggcacgtta
    28441 ctgtttttct ttattaccgg cgggcgcgtg cccagttatc tcggctccag cgcggctttt
    28501 gtcggcgtgg tgatcgccgc aacgggcttt aacggtcagg gcattaaccc gaatatcagc
    28561 attgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg cctggtggtg
    28621 atgaaaatcg gtacgcgctg gattgaacgg ctaatgccgc cggtggtgac gggtgcggtg
    28681 gtgatggcga ttggcctgaa cttagcgcct atcgcggtaa aaagtgtatc ggcttcaggc
    28741 tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc ggtgtttacc
    28801 cgtggcatga tccagcggtt attgattctt gtaggattaa ttgtcgcctg tctgctgtat
    28861 ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct cgtcagccat
    28921 gctgcctggt tcggtctgcc acatttttcc actcctgctt ttaatggtca ggcaatgatg
    28981 ctgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct caaggcggtc
    29041 gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt aggcgatggt
    29101 ctggcaacga tgctttctgg ttctgtcggc agtagcggcg tcaccaccta tgctgaaaat
    29161 atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc tgctgccgtc
    29221 atcgccatgc tgttgggctt ttcaccgaaa tttggcgcgc tgattcatac cattccggcc
    29281 gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc aggcgcaagg
    29341 atatgggtac aaaaccgtgt cgatttaagc cagaacggta atttgattat ggtcgcagta
    29401 accttagtgc tgggcgcggg tgattttgct ctcacgctgg gcggttttac gttgggagga
    29461 atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag aaggttggtt
    29521 gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg ccggggttac
    29581 cggcggtgat ggctttttga cgagcttaag aaaaaaatgc attcgtcatt gattagcccg
    29641 gctagttgtc gcttttgccg tgactgctct tgccaccttt tttacctgct tctgatgctc
    29701 tttcgcggtc ttctgcaaaa ttgccggaac cgcctcgatg gtttgccatt actgacctcc
    29761 gtctgggtat agacatcata ttgctagaat taacgcggcg tatggcggct gccatatttt
    29821 cgctacgtgg gactaagctt agtgaactga cgtgtttcta cccgcgtttg gtaatatcct
    29881 gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa gccgctctta
    29941 tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt gcgtgcttgt
    30001 gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa ggacgtctta
    30061 tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga gtggttagaa
    30121 atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac gatggcacgc
    30181 gcagtcgctg agggtgcaag caaagtggat ggcgctgaag ttgtcgttaa gcgtgtaccg
    30241 gaaaccatgt cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac tgcaccggtt
    30301 gcaaccccgc aagaactggc caattacgac gccattattt ttggtacacc tacccgcttt
    30361 ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct gtgggcttcc
    30421 ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg cggcggtcag
    30481 gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt gattgtcccc
    30541 attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg tacgccgtac
    30601 ggcgcaacca ccatcgcagg cggtgacggt tcacgccagc ccagccagga agaactgtct
    30661 attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa tggctaatct
    30721 tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc ggtgaatggg
    30781 catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc gccgggtacg
    30841 acgaaaaaat ggcggaaaag atttgggaag aaggtagcga tgaagtgtta gtcaaagcct
    30901 ttgctaagac cgacaaagat tcgctttttt ggggcgaaca gaccatcgaa cgtaaaaacg
    30961 tttaaccagc gactcccccg tagcgaacgg gggagttttc tgttatttca ccgcttcatt
    31021 caacacgcta tcaaacttat ccatcgggca gaaaccatca gcgtctatcg ggcaaccgct
    31081 taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg cattacgtaa
    31141 ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg ctttgctgtc
    31201 acgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct ggtcatgcaa
    31261 ctgatacggt ttgaaatcca gcgccgttaa cagagaggca atgttggagt catgcccaac
    31321 taacaccgtg atttttggag cactggtgcg atcggtgacc agggctttgt cgatataact
    31381 gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacaggc tgtcctggta
    31441 accgtttttc agcttcgaca acaccttcca ctgctggtca gatttgattt ctccccaggc
    31501 cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta ccagcgagtt
    31561 gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc taaaggtatt
    31621 tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat ctttatagtt
    31681 aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga gctctttctc
    31741 cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca ccgggttaaa
    31801 ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac accccgggaa
    31861 tgcgccggta ataaagaact gtgcggtcgc aacggtacgt tgcagactat tggcataggc
    31921 ataaacggtg tccggcggcg ggcattcccc cgattttacc atcccctgct ctgccagcca
    31981 ttcacgcatg taatggccca tatacacttc gagtacgcca cctttggtgg tgagttgccc
    32041 accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca cactgccatt
    32101 gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct gtagctgata
    32161 gccttccggt acggtttgtg cctgagcgtt tgaagcgagt aaaactaccc ctgccacagc
    32221 tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc actaaagagt
    32281 gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt tgaacggctg
    32341 tttttatatt catctttaaa aacaggcaag gtttgacgca aactatgcct gattagcccc
    32401 tgcctcgcgt acgccgctga tgattgcacc tgtggctaaa cagaatatcg gcggcgttac
    32461 caatacagag gcaatattta ccaataaaaa agtgtgagac taataacaat ggtttttagc
    32521 atatgcctct gtcattaaat tgcataacga gttaaaaaac caatttgcgc attcctttcc
    32581 ttattgttta actcgtaagc ctgagcaaaa agttcaggag ggaggtaaac gtcataatga
    32641 ttgccagtgt ttattaaata accatactca agatcttcat ctgttgaaaa ggattcattc
    32701 cctaaggaga ttgatgccac attactatta acaataaata atgagcaaat gctatctgaa
    32761 agagcagtga ggaaattttt cacctcatta atcctcttct ctgggtcggt gtctaaaaca
    32821 aggcgcgcct tgttatcatt aaatataaac tcgggtaaat gcagtatgca ttttttgtca
    32881 ttcaagcaac tatcaataaa ttcttttttt aaaaaacaga aagttccatg acataagact
    32941 accctgcccc tgaattttgt tgtcagattg tagatcaacg ccaaataacg tctttacatc
    33001 ttccctgaga ttatagaatg ccgtatcaac aagagcctcg atcgtttgtt tatcaacaat
    33061 tttaataaac tcttcgtata cttgcttttg cgtcttatgg ctgcccagcg cccatgaata
    33121 ctcatgttct tttaaaacca gtatggccct taacagacaa tagccatcgc catctactgg
    33181 agcgtatttt aatccataag ttttattaat ttcactctga ataaaagata taccagacaa
    33241 atcaatacca gaatctaaat cagatattcc cggcgtattt atccattctg gctggatact
    33301 catattttca tcattatcaa aacaataatc agaataaaca tcatcacacg cattaatgtt
    33361 ttcaatatat tccataaagc cataccaata ataaccttta aaatctgtga tagactctga
    33421 acctctaccc ggcaaggata ctctaacaat atcaacatct tcaacatatt gtatagtata
    33481 tgtaatacct cctgaaaccc ctgaataaat ttgtttatca ttagtacgca atgttttcat
    33541 aaataatgat aaatcattat cattctgtat ttcctcaaat tctttttcta gtgaatcgca
    33601 ttccaggcat ggaatgtagt cttcagcttg ttgacgtctg atttctttat acaaccgcat
    33661 aaaacacacc agtgtgtttt caaaaagcgt gattgaatat gttttatttt tttcgtactc
    33721 attttttata tcgttccagg cttgcttcaa ataattattt gcagtgcaac taattccatg
    33781 tatattaccc atatatagcg cctataaaat ttaataaata ataacgcact agtaaaactt
    33841 aaagtgcctg tttcaactat caaaaatcgc tcaccctttt tcacctgttt aaaatatgtt
    33901 cagcgcccca ccttgaggcg acctcctctc cgcgatgatt tcaataacat attctgtgtt
    33961 ggcatatgaa attttgagga ttaccctaca cttataggag ttaccttaca ggggttcctt
    34021 caatttgtgt tgatttacgc gagataacgc tatggaatta aaggattatt acgccatcat
    34081 gggcgtgaaa ccgacggacg atctcaagac aatcaagacc gcctatcgtc gacttgcccg
    34141 caaataccat cctgatgtca gcaaagaacc ggatgccgaa gcccgcttca aagaggtcgc
    34201 tgaagcctgg gaagtgttaa gtgatgaaca acgtcgcgct gagtatgatc agatgtggca
    34261 acatcgcaac gatccgcaat ttagccgtca gttccagcat ggcgacggtc agagttttaa
    34321 cgccgaagat tttgacgata tcttctcgtc aattttcggt cagcatgccc gccagagccg
    34381 tcaacgcccc gccgcacgcg gccacgatat tgaaatcgaa gtggcggtat tcctcgaaga
    34441 aacgcttact gagcataagc gtaccatcag ctataacctg ccggtttata acgcctttgg
    34501 catgatcgaa caggaaatcc cgaaaacgct gaatgtgaag atcccggcag gcgtcggcaa
    34561 tggtcaacgc atccgcctga aaggccaggg gacgccgggc gaaaacggcg gtccaaatgg
    34621 cgatttgtgg ctggtgattc atattgcgcc acatccgctg tttgatattg tcggccagga
    34681 tctggaaatt gtggtgccgg ttagcccgtg ggaagcggcg ctgggtgcta aagtcaccgt
    34741 tccaacactg aaagaaagca ttttgctgac tatcccgcct ggcagccagg ccgggcaacg
    34801 attgcgcgtt aaaggcaaag gtctggtgag caaaaaacag accggcgatc tgtatgcggt
    34861 actgaaaatc gtgatgccgc cgaaaccgga tgaaaacact gccgcgctgt ggcagcaact
    34921 ggcagacgcc cagtcgtctt ttgatccacg taaagattgg gggaaagcat aatggctaat
    34981 gttacggtga cttttactat taccgaattt tgcctgcata ccggcatctc tgaagaggaa
    35041 ttgaatgaaa ttgtcggttt gggggtggtt gaaccgcgtg agatccagga aacaacctgg
    35101 gtatttgacg accatgccgc catcgtggtg caacgcgcgg tgcgcctgcg tcatgaactg
    35161 gctctggact ggccggggat cgcggtggcg ctgacgttaa tggatgatat tgcgcacctg
    35221 aagcaggaaa accgcctgct gcgccagcgg ctttcccggt ttgtagctca tccgtgatgt
    35281 gtgtttgccg gatgtgatga cgcatcttag cctgcctgca aagagtactt ttcctgtggg
    35341 ccggataagg cgtgttcacg ccgcatccgg cggcggtgcc ctaatgcctg atgcgacgct
    35401 aaacgcgtct tatcaggcta acaaacgacg ttatctgttt tggtggtcgc cctcaactaa
    35461 caccagcaat aactggctta gtgccgcgta aaaaccaaag ctgtcatact gatggcaacg
    35521 cgcaacaaac tctggtaacc attgccgcaa cgccgtgagt gttttttgcc gtaaactgtc
    35581 gattcttcgc gcaggaaccg tcccctctcc cagcgaaaaa tgcagatgac tcaacaactc
    35641 gagatagatc gccagatgat ctgtcggttc gttgaaattg ccgctggttt ccatccctgc
    35701 ctcaactaac aagcgtttaa tctcttgctc gtcctgtttg taggccgatg catacggcag
    35761 cgccgcttgt ttgtcagtca tcagaaacag gccgcaaaag tcagcggcca gttccagacg
    35821 ggcatcgtca cgtactgtca gagtggcaac acggttttcc agctcatcca ccgccgcagt
    35881 gagcggcggt tcgcttttca gcaacgaaaa ccattcagcc atctgcgcac tggcgatttg
    35941 cgtcagttgt tcatcgtcca gttcacggga gaacaactgc gccagccagg cgtaaacaca
    36001 ggctatctgt tgtgctgtca gcgtggtcat aatttcacct gcgacgcggg aacatattcg
    36061 cactgcgcca ccatctccac ggggccgtta aacgccgtta cctgctccac tgttccgttg
    36121 cacttctcaa tttccaccag cgtagtgtgc gcactggtcg cctgcgccag ctgcgaagta
    36181 ccgatgtcaa tggtcaacac gttggggtta ccgtatttgc acagcgcacc aggctcgccg
    36241 cctttatctg gatcgtgcca tgccccttcg tgaattcgcg ccacgccggg tgcatagcgg
    36301 tcagaaacta ctgcccctgc caacacctga ccgcgagcgt taaagacgcg taccacatca
    36361 ccgttacgaa taccgcgcgc gctggcatcc tgcgggttaa tgaacactgg ctctttaccc
    36421 gctaccgtat attgctgacg cagagtttcc gactcacata actgcgagtg aagtcggaaa
    36481 tccggatgca cagattgcag atgcaacgga tacgtctgcg agccgggccc accgtgggag
    36541 cgttcgattt tctcaaacca catcggatgc ccctgacaat cgtcgtagtt catatcggcg
    36601 atggttttcg aatagatctc aatcaggcca ctcggcgtgc ccagcggttc gagatctgga
    36661 tcttcgcgga atgcctggtg gcgaacaaac atctgcggat ggtcaaactc gacgtactct
    36721 ttgttattcc agaagtcatc aaacgctggc aaatgaacgc cgcgtccttt gccttgctga
    36781 acaccttcct gccagatgcg tttcagccag cccatttcgt ccagcccttc ggtaaaggct
    36841 tcttcgcgat taaagcggcg gcacagctcg cggaaaatat caaagtcgtt gcgcgcctcg
    36901 aactgcggcg gcacgacctg tttcatggcg ataatgccac ggttggagtg gttgccgtac
    36961 tggtcgagat cgttacgctc aaactgcgtg gtcgcaggca gcacgatatc ggcaaagcgg
    37021 caggttgagg tccactggtt atctatggcg ataaccgttt ccagtttgcg ccagccttca
    37081 ataatgcggt tgatctgctg atggcgatgg aaggggttag ttccggcaaa aatacacatt
    37141 ttcagcggcg gcagttttac cgatttaccg ttccagttga ttactttccc cggttcgagg
    37201 atcgcatcga taaaacgggc aatcggaatg gtgctgctgt agcctttgta atcactgttg
    37261 tcgtgaacag gcggaatcga cgtagagccg gagaaaccac tcagaataac gcctttacgc
    37321 cccggcgtgc ctgcgccgtt atagtgccag ccaaaaccaa agccaccacc tggcaggcca
    37381 atttgcccca gcatcgccgc cagaaccaca atcatccacg cccactgttc accgtgctgc
    37441 atacgctgta cgcaccagcc agcaataatc tgcgttctgt tcgccgccat ctgccgcgcc
    37501 agtccacgaa tggtttcggc atcaatgccg gtcagttttt cagcccatgc ggcatctttc
    37561 ggctgaccgt ctttctcacc cagcagatac ggcaggaact gctcaaaacc cacacagtag
    37621 ttagcgagga agtttttgtc gtacaggttt tcactgtaca acgtatgcgc cagcgccagt
    37681 tgcagcggca cgtcagtttg cgggttaacc gcaatgtgct tcacatgctc gcgccccaga
    37741 tactcatggg tggatgtaac aaccggatcg atgctaatga cctcaatttc accggcggcg
    37801 actttcgctt tcaactgcgc gtaatattca taaacatcgt gatccgggca ccaccagttc
    37861 gcttgctggt ttttcagcaa atcggagccc cacagcacaa tggttttgct gttctgcaat
    37921 accagcggcc aggaggtttg ctgttcatac acttccatcg aaccgactac gcgcggcagg
    37981 atcacctgcg cagcaccagt agagtaatct ccgcccgtac caacgctatt accatgcaag
    38041 gcaatagctt tcgccagcat ccccgaagcg ttatggaaca tccccgtcga ttgccaacca
    38101 ctggcggtca gcaaggcact cggcccgtga gttttctgta cgcgttccag ttcttcatag
    38161 aacatgtcga gggcttcatc ccagctcaca cgcacaaaac ggttatcacc gcgctgggag
    38221 gtgtcgctca gatggcgctt acgcagccag tccacgcgta ccatcggata acgaatacgc
    38281 gccgcgttgt gtacatgatc cggcaatccg gcaatcattt tcgacggata tttatccagt
    38341 tcgaacggtt ttgccgccac aaagcgacca tccttcaccg tcgcgcggat agccccccag
    38401 tgcgacccgg taagaatgcc ctctttcgag atgacagcct cagtcgccgc ttgcgccgca
    38461 gtcgcacggc gcgacgttaa caatgacggc cccagcatcc cggcgacggt taagccgccg
    38521 agttgtgcca gaaaacgccg acgtgatgcc tgaaagagat cgttattgtt cattattttt
    38581 cttccttctt atcgccgtga gccttacctg cggtgtcaga cgcattcatt tgcagatatt
    38641 tcaacaaggt gcgttcttca cgtttatcga ggctggtaaa gccgatcatg ccgttgagcg
    38701 tgccaatcca gccgttagcg tcaaagtgag agatttccgg tgcgccgtgg cactggttac
    38761 aggtgccgtt gtacaacgaa tccgcataag cccagatcgg tttgatatcg ttcaccatgt
    38821 cacctttctt catccacgcg gtggcctgca acttgctcca ctcggtattg gtgtcggcaa
    38881 cggtggtttt ctccagcgtt tttacctgct gctgcacatc accacgaatc gaggcaacaa
    38941 agatgcgttt acctgggaat tgggtgagta cacgctgacg tccggcgctt tctgtccagc
    39001 cggtaatttc aatttgcagc cagtcgccgt cacgtttaag gactttcact tccgaggcag
    39061 gcagcagaga accagaggct tctttatcgc ctttcgccgc ataaattggc ttaatatcaa
    39121 tagagtacag cgtgtcacca ctgtcattag caccggcgcg cagctcatcg aactgcttac
    39181 ggaagccgct actcatatcc ggtaactggt gggcaatacc tttatgacag tcgatgcagg
    39241 attgattatc tttcgctgcc accttcatct gacgtgccgc ttcaggatgc tgcttcgcat
    39301 gatccatcgc atcgtagtta tggcaggagc gacaggttgc cgagttgttt tctttcattc
    39361 gcgcccattc acgctcggca agttccgcgc gtttggcttc aaatttttca ggtgtatcaa
    39421 tggagtgggc aataaaggtc tggtagatat cgttgctcgc ttccagtttg cgcttcacca
    39481 tgcctggaat atccggcggg atatgacagt catggcattc agctcgcacg ccggaggcgt
    39541 tctggaaatg caccgactgt ttatattctt catacactgg ttgcatactg tggcaactga
    39601 cacaaaattc ggttgtgctg gtgactttga tcccaacgtg tggcaataca atcagcgcaa
    39661 tgccaatcac aatcccaata gcgaccagcg ccagtaccga ccaacgagca ctgggtcggc
    39721 gtagcgcgtt ccagagtttc cgcataatag cccctgtaaa attatggttt agtgaagcga
    39781 tcttaatgag taaatatgaa cagcggcact ggtcaggatg aacggcttac ggcagaatat
    39841 gaacagatat gaacagaatg agtaaaaccc tctgatgcca catcacattg ttattgttga
    39901 agatgagccg gttacccagg cgcgattaca atcctacttc actcaggagg ggtataccgt
    39961 ttccgttacg gcgagcggtg ccgggctacg ggaaattatg cagaatcagc cggtggattt
    40021 aattctgctg gatatcaact tgcccgatga aaatggcctg atgttaaccc gcgccctgcg
    40081 agaacgctca acggtgggga ttattctggt taccggacgc agcgatcgga ttgaccgtat
    40141 tgttgggctg gaaatgggcg cagacgatta cgtcaccaaa ccgctggaac tgcgcgaact
    40201 ggtagtacgg gtaaaaaatc tgctctggcg aatcgacctc gcgcgacaag ctcaaccgta
    40261 cactcaggac aactgctatc gctttgcggg ttattgcctg aatgtgtcgc gccatacgct
    40321 ggagcgggat ggtgagccga ttaaactgac ccgcgcagag tatgaaatgt tggtggcatt
    40381 tgtgaccaat ccgggcgaaa ttctcagccg tgaacgtctg ctgcgtatgc tctctgcgcg
    40441 tcgggtggaa aaccctgacc tgcgcaccgt cgatgtgtta attcgtcgtt tacgccataa
    40501 actcagcgcg gatttactgg tgacgcaaca tggcgaaggt tatttcttag ccgctgatgt
    40561 gtgctgataa aaatagaccg gacgaaatcc cccaggcgac agcgagcggc ggatatgttc
    40621 gcggtcggca tttttcggcg tcagaactaa aatcggtggg ctgacattat cagacaccga
    40681 ttgcccctgt aattgcctga tggcctgctc aactgccagt tccccctgcc agaccatttg
    40741 atcgctggca gccataatca ctcttccccg cttcagcccg cgatacacct gatgtgaaag
    40801 ataaaacgac accacggtaa gcggcgtttt caggttacgc ccttccccca ttgccgcctc
    40861 tgccgcaatg gccgttccgg caacgacgtc gatttctgga tggcgttcca gcatctcctg
    40921 caacaggtta cgctggattt caatatcgtt atcaccaagc gcaatatcaa caatacgcac
    40981 cgggcttccg gcaatggctg cgcgaaaacc ctcgaccatc tccttactgc ccccggcgtt
    41041 atcgggtccg ggcatcaaca gcacattcag tggtttaccg tgcgcccatt gcaccagata
    41101 tcgcccaggt tgatagccca tctgaaacca gggcacacca acgcggcttt tcacctgggg
    41161 agcatcaata gcatttacca gctcgatcac cggcagattt gctacttgct tttgcagatc
    41221 gggaaatgac gtcgtgctac taccgagcaa aatggcctct gcgccccact gtttacactg
    41281 gtcgatttgt gcttgctggg tagccaactg gctgtagccg cctgcctcca gcacttttaa
    41341 atccacaccg tagcggcgag cagcctcctg cataccatag ttcaacgata accaatatga
    41401 atctttcagg ctgggataaa gcgcgcacag tttccatacg cgtttggctt ttagcggcat
    41461 agaggcttgc accgtgaaat gctgcgcatc atgccagcgc aacaggttat cagccgaaaa
    41521 tgccggcaac atgaaaaggg aaagaagtaa aaatagcagc acgcgcatga tagcctcatc
    41581 aataataagg ctttatgcta gatgcattcc gctttgcgac tcaacatttt tcaccttaag
    41641 tgcaccgacc gtgaatttaa ccctgacccg aagactctgg atgggctttg ccctgatggc
    41701 gctgttaacc ctgaccagta ccctggtggg atggtacaac ctgcgcttta tcagccaggt
    41761 ggaaaaagac aatactcagg cattgattcc taccatgaat atggcgcgcc agttgagcga
    41821 agccagcgcc tgggaacttt tcgccgcgca gaacctgacc agtgccgata acgaaaagat
    41881 gtggcaggcg caggggcgaa tgctcaccgc acaaagcctg aagattaatg cgttgctgca
    41941 agcgttacgg gaacaaggtt ttgataccac cgctattgaa caacaggagc aggagatctc
    42001 ccgttcgtta cgtcagcaag gggaactggt ggggcggcgt ctgcaactgc gccagcaaca
    42061 acagcgactc agtcagcaga tagtcgctgc cgccgatgag atcgcacgcc tggcgcaagg
    42121 tcaggcgaat aatgcggcaa cctccgccgg agcgacccag gccgggattt acgatttgat
    42181 cgaacaagat cagcgtcagg ctgctgaaag tgcactcgat cggctgattg atatcgatct
    42241 tgagtatgtt aaccagatga atgaactgcg ccttagcgct ctgcgggtgc agcaaatggt
    42301 gatgaatctg gggctggagc agatccagaa aaatgcgcca acgctggaaa agcagctcaa
    42361 taatgcggtg aaaattctgc aacgtcggca aatacgcatt gaagatccgg gagttcgtgc
    42421 tcaggtcgca acaacgttaa ctaccgttag ccaatatagc gatttgctgg cgctgttcca
    42481 gcaggacagt gaaatcagca atcacctaca aactctcgca caaaataaca tcgcccagtt
    42541 cgcgcagttt agcagcgaag tcagtcagct ggtcgacacc attgagctgc gtaatcagca
    42601 cggactggcg catctggaaa aagccagtgc acgcgggcaa tacagcctgt tattgctggg
    42661 gatggtttca ctttgcgcac tgattctgat cctctggcgc gtggtttatc gctcagtcac
    42721 gcgtccactt gccgaacaaa cgcaggcgct gcaacggctg ctggacggtg atattgactc
    42781 ccctttcccg gaaaccgctg gcgtacggga gctggacacc atcgggcggc tgatggatgc
    42841 gtttcgcagc agtgttcatg cactgaatcg ccaccgcgaa cagctggcgg cgcaggtcaa
    42901 agcgcgtaca gctgaattgc aggaactggt gatagaacac cgacaagcac gggcggaagc
    42961 agaaaaagcc agccaggcaa aatcggcgtt tctggcggcg atgagccatg agatccgcac
    43021 accgctgtac ggtattctcg gcaccgcaca gctactggca gataaccccg cacttaacgc
    43081 ccagcgtgat gatttgcggg caattaccga ctccggcgaa tcgttgctga ccatcctcaa
    43141 cgatattctc gattattccg ctatcgaagc aggtggcaag aatgtttcgg tcagcgatga
    43201 accctttgaa ccgcgaccgc tgctggaaag taccctgcaa ttaatgagtg gacgggtgaa
    43261 aggtcgcccg attcgcctgg caacggcaat tgccgacgat gtaccgactg cgttaatggg
    43321 cgatccgcgg cgtattcgtc aggtcattac caacctgttg agcaacgccc tgcgttttac
    43381 tgacgaaggg cagatcgttt tacgtagccg tactgatggt gagcaatggc tggtcgaagt
    43441 ggaagacagc ggctgcggta ttgatcccgc gaaactggca gaaatcttcc agccatttat
    43501 tcaggtaagt ggcaaacgcg gcggcaccgg gctggggctg actatcagta gccgtctggc
    43561 ccaggcgatg ggcggcgaac tgagcgccac cagcacgccg gaggttggaa gctgtttttg
    43621 tttacgcttg ccgttacgtg ttgccacggc acccgtgcca aaaacagtca atcaggcggt
    43681 gcgtcttgac ggtttacgtt tgctgttaat tgaagataac ccgctaaccc agcgaattac
    43741 cgttgagatg ctgaacacca gtggtgcgca ggttgttgct attggcaatg ccgcgcaggc
    43801 tttagagaca ctgcaaaata gcgaaccgtt tgctgccgca ctggtggatt ttgatctgcc
    43861 ggatgtcaac ggcattacgc ttgcccgaca actggcacga caatatccgt cgctggtttt
    43921 gattggcttt agcgcccatg tcattgacga aacgctgcgc cagcgtacca gttcactatt
    43981 tcgcgggatt atccctaaac cggtgccgcg tgaagtgctc ggtcagttac tggcgcacta
    44041 tctccaactg caagccaata acgatctacc actggatgta tcgcaactca atgaagacgc
    44101 tcagttaatg gggacggaga agatccacga atggctggca ttatttacac aacatgccct
    44161 gccgcttctc gatgaaatcg acattgcccg cgccactcag gatagcgaga aaataaagcg
    44221 tgccgcgcat cagctaaaaa gcagttgctc gagtctggga atgcgtagcg ccagccagct
    44281 ctgcgcacaa ctggagcagc agccattatc tgcccccctt ccacacgaag aaattacacg
    44341 cagtgttgcc gctctggaag catggttgaa taagaaagac ctgaacgcga tttgaatcaa
    44401 tatcagccag ttaagaataa ctagaatagt ggcatctggt atgcaactta acggactggc
    44461 aaaatcatgg cagagaaaaa aagaacccgc tggcagcggc ggccaggcac gacgggcggc
    44521 aaattaccgt ggaatgactg gcacaatgcc acgacctggc gtaaagcgac gcaattattg
    44581 ctgttggcaa tgaatattta tattgccatc acgttctggt attgggtgcg ctattacgaa
    44641 acggcaggta gtacgatatt tgtcgccaga ccgggaggta tcgaaggctg gctaccgatt
    44701 gccggtctga tgaatctgaa atatagcctt gcaacaggtc agttaccgtc cgtccacgcc
    44761 gccgcgatgc tgttattggt cgcttttatc gtcatcagtc tattactcaa aaaggccttt
    44821 tgctcatggt tatgcccggt tggtacgctt tctgaattaa tcggcgctct cggtaacaaa
    44881 ctgtttggtc ggcaatgtgt ccttccccgc tggctggata ttcctctgcg cggtgtaaag
    44941 tatttgctgt tgagtttttt tctctatatc gcgttattga tgcctgctca ggcgattcac
    45001 tattttatgt tgtcgcccta cagcgtggtg atggacgtta aaatgctcga tttctttcgt
    45061 catatgggga ccgcgacatt aatcagcgtg accgttttgc tgattgccag cctgtttatt
    45121 cgccatgcct ggtgtcgtta tctttgccca tatggcgcgc tgatgggcgt ggtttcgcta
    45181 ttatcgccgt ttaagattcg tcgcaatgcc gaaagttgta tcgattgtgg caaatgcgca
    45241 aaaaattgcc catcacggat cccggtcgat aaattaattc aggtacgaac agtggaatgt
    45301 accggctgta tgacttgcgt agagtcatgt ccggtagcct caacattgac cttttcactg
    45361 caaaaacctg cggcaaataa aaaagccttt gcgttgtctg gctggttaat gacgctactg
    45421 gttctgggga ttatgtttac ggtgattggt tacgcaatgt atgcaggagt atggcaaagc
    45481 ccggtgccgg aggaattgta ccgacgctta attccgcaag cgccaatgat tggtcactaa
    45541 attagcaaca agcggaacat cacctcgcac ggggtgatgt tcacaaaatc acattatttt
    45601 gattttgaca tcataacttt caagcccggt cattttttct cgcgcggtga agcgaatttc
    45661 agagacttct ttccctgtat tcttgttcag ctcggcgatt ttttgcgcga taaagtcggc
    45721 gatttccgtt tcggcttgtt ttttaactct tcaatattca ctgttaacct cttctggtct
    45781 taaaagacag caacacgcca gtaaggtgtt ggttcccggt accctggtca ggtaagaaat
    45841 gttataagcc atctccccga aagattcaat aatcaacagg ttatcgctta ctatacatac
    45901 agcgttgaaa aaaaataaga cagatataaa aaaggctcca caagggagcc tgatattatt
    45961 ttgtgaaatc cagcggtgca tggtcagtgc gtatttgcag cgcgacgtta tcaaaggtga
    46021 tcatcgtcga tttacaaaaa atacacttag cgcccaaagg gtttcgctcc gtcacatcga
    46081 atgccgatgt gcggtactgt gaaccatgac aacatggaca gcgaaaatgg agatgttgag
    46141 taataacggc aaccttagag cgtaactacg ttcgctgccg cggggccacg ttgaccctgc
    46201 tcaatagaaa attcaacttt ctgattttcg ttcagcgtgc ggaattcatt gctctggatg
    46261 gcggtgaaat ggacgaaaac gtctttgctg ccatcatcag gagtgataaa gccaaaacct
    46321 ttatctgcgt taaaccattt tactaaacca gtcattttat tagacatatt ttacttcctt
    46381 cattttgggc ctttcggcaa acagggcttt aatacagaaa ctacttagtg ttcaggagag
    46441 agactcaaaa gaaggggata atgcctgata atgagaactg ctttagtaaa ctactttgta
    46501 tgctgtctgt ctttccaacc gacgcagcta ttaacgcatg ataattgaga ttaagcaaat
    46561 tttattttag ccgtccggcg gtcttaaata tcgctggctt tattttatta accccgtata
    46621 gtgcagcgca gtattttcag taaggaattt gtttgtcccg taaaatgaca ggaattgtca
    46681 aaacctttga tcgcaaaagc ggcaaaggat tcattatccc ctccgacggt cgtaaagaag
    46741 ttcaggtcca tatttccgca ttcactcccc gcgacgcaga agtgcttata ccaggattac
    46801 gcgtggaatt ttgccgcgta aatggcctgc gaggaccaac agcggcaaat gtttatctct
    46861 cttaatatga cgctgcttgt ttaaagctga ctggttttat tggcatagtt caatagtcaa
    46921 caccatgtat tataacacaa gttacttttt caccggaaaa ctaatccatt taaccgaata
    46981 tctctacaac taatagttat aaatgccact taacagcgag ttatagtacc acttgaaact
    47041 atattgccaa caatagttaa tagctttctt aacaattttc ttatctttaa tttgtatttt
    47101 gctaagattt atcttatgct cacaggtcgt atttccagag gggaatagtt aacaatctta
    47161 tatttccctc tgcgcgcctt tctcacccgc tgtatatagt gcaagtggta tgaggaaagg
    47221 gagatttcac aaggatgtta tactcaaagt agacaatcct gatctcaagc gtacgtattg
    47281 tccatgcgat gaataaagga aaagttatga aacacaagct ttctgcaatc cttatggcct
    47341 tcatgttgac gacgccagct gcgtttgctg cccctgaagc agcaaacggg accgaggcaa
    47401 cgacgggtac tactggcacc acaacgacca ctaccggtgc aaccacgacg gctgctacca
    47461 ctggtggcgt ggctgctggc gctgtcggca cggcgacagt tgtcggtgtg gcaacggctg
    47521 ttggtgtggc gacgttggca gtagttgcag caaatgacag tggcgatggc ggttctcata
    47581 acacctcaac caccacgtcc actacgcgct aattctgctt aaacgcgtcg aaattaaagt
    47641 taaataatac ccggttgcga tattcgtacc gggctttaga gatttgcatt tttttaatta
    47701 acctttcaag gattaaaggt gcgccctctt attttatcga ttttcgcact atttcttgcg
    47761 ggatgtacgc acagccagca aagtatggtc gatacatttc gcgccagcct tttcgataat
    47821 caggatatca ccgtagcgga tcagcagatc caggcgttgc cttattccac gatgtattta
    47881 cgccttaatg aagggcaacg aatctttgtg gtactgggat atatagaaca agaacaaagc
    47941 aaatggttat cccaggataa cgccatgctg gttacccaca atggacgtct tttaaagacc
    48001 gtcaaactta ataataatct gctggaagtg acaaattccg ggcaggaccc tctgcgtaac
    48061 gcgctggcaa taaaagacgg cagccgctgg acgcgcgata ttctctggag tgaagacaac
    48121 cattttcgct ctgcgaccct gagttccact ttttcctttg ctggattaga gacgctgcat
    48181 attgcgggtc gcgatgtgct gtgtaatgtc tggcaggaag aggtgacttc cacgctacca
    48241 gaaaaacagt ggcaaaacac attctgggtc gattcggcta ctggccaggt tcgtcaaagt
    48301 cggcaaatgt taggcgcagg ggttattccc gtagaaatga cgtttcttaa acccgcacca
    48361 tgaataaatt acagtcgtat ttcattgcca gcgtacttta cgtaatgaca ccccatgcct
    48421 ttgcgcaagg aacggtgact atttatctgc ctggcgaaca acagacactt tccgttggcc
    48481 ccgtggagaa tgttgcccag ttagtgacac aaccgcaact gcgcgatcgc ctctggtggc
    48541 ctggtgccct gctgaccgac tccgcggtga aagctaaagc gcttaaagac taccaacatg
    48601 tcatggcaca gcttgcttcc tgggaagcag aagctgacga cgatgtggcg gcaacgatta
    48661 aatccgtgcg ccagcagtta ctcaatctca atattactgg tcgcttaccg gtcaaactgg
    48721 accctgattt tgtacgagtg gatgagaaca gcaatccccc gctggtcggg aactatacgc
    48781 tctataccgt acagcgcccg gtgactatta ctcttctggg tgcggtttct ggggctgggc
    48841 aattgccctg gcaggcaggc cgtagcgtta ccgattatct gcaagatcac ccccgtcttg
    48901 caggcgcgga caagaataac gttatcgtga ttacgcctga gggtgagact gtcgtcgccc
    48961 ctgttgccct gtggaataaa agacatgtgg aaccaccgcc tggcagccag ctttggttag
    49021 gcttttctgc gcatgtactg cccgaaaaat atgctgattt aaatgaccag attgtgtctg
    49081 ttttaacgca acgtgttcct gactgatgaa gaagaattct tatcttttaa gctgcctggc
    49141 cattgccgtc tccagtgcct gtcatgctga agtattaacc tacccggatc cgctgggttc
    49201 gtcgcaatcg gactttggcg gcacaggatt gttgcagatg ccaaatgcgc gcatcgcacc
    49261 ggaaggtgaa ttcagcgtca actaccggga taacgatcaa taccgtttct actccacctc
    49321 cgtggcgctg ttcccatggc tggaaggcac cattcgttat acggatgtgc gcacacgtaa
    49381 atatagccag tgggaagatt tcagcggcga tcagtcatac aaagacaaat cattcgattt
    49441 taaacttcgc ctgtgggaag aaggttactg gctaccgcaa gtggcgtttg gtaaacgtga
    49501 tattgctggt acgggtctgt ttgacggtga gtatctggtg gccagcaagc aagcggggcc
    49561 atttgatttc accctcggga tggcatgggg ctacgccggt aatgcgggca atattaccaa
    49621 cccgttttgc cgggtgagcg ataaatattg tcatcgcgca gagtctcacg atgcgggcga
    49681 tatcagcttt agcgatatct ttcgtggccc ggcttccatc tttggcggca ttgagtatca
    49741 aacgccgtgg aatcccctgc gtctgaaact cgaatacgac ggcaacaatt accagaatga
    49801 tttcgctggc aaactgcctc aggcaagcca tttcaacgtc ggtgcagttt atcgcgctgc
    49861 cagctgggca gacctcaacc tgagttatga acgcggtaac acgttgatgt ttggtttcac
    49921 tttacggacc aatttcaacg atctgcgccc tgccctgcgc gatacgccaa aaccggcata
    49981 tcaacctgcg cctgaatctg aaggattgca gtacaccacg gtagcaaacc aacttaccgc
    50041 cctgaagtat aacgcgggct ttgacgcgcc agaaattcag ctacgcgata agacgctgta
    50101 tatgtctggt cagcaataca aataccgtga ctctcgtgaa gcggtcgatc gtgccaaccg
    50161 gattctggtg aataacctgc cgcaaggcgt tgagaagatt agcgtgacgc aaaagcgcga
    50221 gcatatggcg atggtgacta ccgaaaccga cgtagccagc ctgcgcaaac agctggcagg
    50281 tacagcgcct ggtcaatcag agccactgca acaacaacgt gttgaagctg aagatctttc
    50341 tgcctttggt cggggctacc gtattcgtga agatcgcttt agctactctt tcaacccaac
    50401 actttcacag tcgctgggcg gcccggaaga tttctatatg ttccagctgg ggctgatgtc
    50461 cagcgcccgc tactggttta ccgaccacct gctgcttgat ggcggtattt tcaccaatat
    50521 ttacaacaac tacgacaagt ttaagtcttc gctgttgccc gcggactcta ccctgccccg
    50581 cgtgcgcacg catatccgtg attacgttcg caatgacgtt tatctcaaca acttgcaagc
    50641 gaattacttt gccgacttag gcaatggttt ctatggccag gtgtatggcg gttatctgga
    50701 aacgatgtat gccggtgtcg gttccgagct gctttatcgc ccgctagatg ccagctgggc
    50761 gctgggtgtg gacgttaact acgtgaagca gcgtgactgg gacaacatga tgcgcttcac
    50821 cgattattcc acgccaactg gtttcgtgac ggcttactgg aacccgccga cgctcaatgg
    50881 cgtactgatg aaacttagcg ttgggcaata tctggcaaaa gataaagggg caacgatcga
    50941 cgtcgccaaa cgctttgaca gcggcgtggc ggtaggggta tgggcggcaa tcagtaacgt
    51001 atctaaagat gactacggcg aaggcggctt tagtaaaggt ttttatatct cgattccgtt
    51061 cgacttgatg accattggac ctaaccgcaa ccgcgcggtg gtttcgtgga caccattgac
    51121 gcgtgatggt ggacaaatgc tgtcacgcaa ataccagctc tatccaatga cggcagagcg
    51181 agaagtaccg gttggacaat aacctgacgt aattcataac attacattga ctgataaata
    51241 taaaccaatg aaaaagaaca tttttaagtt ttcggtactg acattggctg ttctgtcttt
    51301 gacagcatgt acccttgttc ctggtcagaa tttatcgacc agtaacaagg atgtcattga
    51361 actacctgac aatcagtatg acctggacaa aatggtcaat atctatcccg tcacgccagg
    51421 attgattgat cagttgcgcg ccaaaccgat tatgtcgcag gctaacccgg agctggagca
    51481 gcaaatcgcg aactatgaat atcgtatcgg cattggtgat gtgttgatgg ttacggtctg
    51541 ggatcacccg gaactaacca cccccgcagg gcaataccgt agcgccagtg ataccggcaa
    51601 ctgggttaac gccgatggtg cgattttcta cccctatatt ggtcgcctga aagtggccgg
    51661 gaaaacactt acccaggtac gcaacgagat tacagcgcgt ctggattcag tcattgaaag
    51721 cccgcaagtc gatgtcagcg tcgcggcgtt ccgctcgcaa aaagcgtacg tcaccggtga
    51781 agtcagcaaa tcgggtcagc agccgattac caatattcct ctgaccatca tggatgcgat
    51841 taacgccgcg ggcggtctga ccgcagatgc agactggcgc aatgtggtgc tcacccagaa
    51901 tggcgtaaag accaaagtga acctctatgc gctgatgcag cgtggcgact tgcgccagaa
    51961 caaactgctg cacccgggag atattttatt tattccgcgc aacgatgatc tgaaagtctt
    52021 cgtgatgggt gaagtgggta aacagagcac gctgaaaatg gaccgcagcg gtatgaccct
    52081 ggcggaagcg ctgggtaacg ctgagggtat gaatcaggat gtcgctgatg cgacggggat
    52141 ttttgttatt cgtgcaacgc aaaacaagca gaatggcaaa atcgccaata tttatcagct
    52201 gaatgcgaag gacgcatcgg caatgatact cggaacggaa ttccagcttg aaccttacga
    52261 cattgtttat gtcactaccg cgcctctggc tcgttggaac cgtgtgattt ccctgctggt
    52321 accgacgatt tccggggtgc atgacctgac agagacttca cggtggattc aaacatggcc
    52381 caactaaaat ttaactcaat cctggtggtt tgtaccggca acatttgccg ttcgcccatt
    52441 ggcgagcgct tgctgcgtaa acgtctgccg ggcgtaaaag tcaaatcggc aggtgttcat
    52501 ggtctggtaa aacaccctgc agatgcgaca gcggcagatg ttgccgctaa tcatggcgta
    52561 tctcttgaag gacatgccgg acgtaagctc actgctgaga tggcacgaaa ttacgatctg
    52621 attctggcaa tggagtcgga acatattgct caggttaccg caatcgctcc cgaggtccgc
    52681 gggaagacaa tgctgtttgg gcaatggctg gaacagaaag agatcccgga tccctatcgt
    52741 aaaagtcagg atgcatttga acatgtctac ggaatgttgg agcgcgccag tcaggaatgg
    52801 gcgaagcgcc tcagccggta atttgagttt ataaaatacg atgacaacta aaaatatgaa
    52861 tacgccacca ggcagcactc aggaaaatga gatcgatctg cttcgtctgg tcggcgagtt
    52921 atgggatcac cgtaagttta ttatcagcgt gaccgcgtta ttcacgctga tcgctgtcgc
    52981 ttactcgctg ttaagcacac caatttatca ggcagatact ctggtccagg ttgagcaaaa
    53041 acagggcaac gccattctca gcggcctgag tgatatgatc cctaactcat cgcccgagtc
    53101 tgcaccggag atccaactgc tgcaatcgcg catgattctc ggtaaaacca ttgctgaact
    53161 gaatctgcgc gacatggttg agcagaagta ttttccgatt gtgggtcgcg gctgggcgag
    53221 attaaccaaa gagaaaccag gtgagctggc gatcagctgg atgcatattc cacaactgaa
    53281 tggtcaggat cagcaactga cactcacggt tggggaaaac ggccactata cgctggaagg
    53341 tgaagagttc accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg gcgttgcgct
    53401 gactatcgcg gatattaagg ccaaaccagg aacacagttt gtcctgagcc aacgtaccga
    53461 actggaagcg attaacgcgt tgcaggaaac ctttaccgtt agcgaacgca gtaaagaaag
    53521 cgggatgctg gaacttacca tgactggtga tgatccccag ttgattactc gtattctgaa
    53581 cagcatcgct aacaactatt tgcaacagaa tatcgctcgc caggcggcgc aggattcaca
    53641 aagccttgaa ttcttacagc gccagttgcc agaagtgcgc agcgagctgg accaggcgga
    53701 agaaaaactc aacgtttatc gccagcagcg cgattcggtt gaccttaacc tggaagccaa
    53761 agccgttctt gagcagattg tgaacgttga taatcaactc aatgagctga ccttccgcga
    53821 ggcagagatc tcccagctgt ataagaaaga tcacccaact tatcgtgcgc tgctggaaaa
    53881 acgccagacg ctggagcaag aacgcaaacg cctgaataag cgggtatcgg ctatgccttc
    53941 cacccaacag gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg cggtatatct
    54001 gcaattactt aaccgccagc aggagttgag tatttcgaaa tccagtgcca ttggtaacgt
    54061 gcggattatc gacccggcag tcactcagcc gcagccagtg aaaccgaaaa aagcgttgaa
    54121 tgtggtgctt ggttttattc ttggcctgtt tatttcggtg ggtgccgtgc tggcgcgtgc
    54181 gatgttgcgt cgtggtgtag aagccccgga acaactggaa gagcacggca tcagcgttta
    54241 tgccaccatc ccgatgtccg agtggctgga taaacgtacc cgtctgcgta agaaaaattt
    54301 attttctaat cagcagcgcc atcgtactaa aaatatcccc ttcctggcgg tggataaccc
    54361 ggcggattct gctgtggaag ccgtacgtgc gctacgaacc agtctgcatt tcgctatgat
    54421 ggagactgag aataacattc tgatgatcac cggtgcgacg ccagacagtg gtaaaacgtt
    54481 tgtcagttca actctggcag cggtgatcgc ccagtccgat caaaaagtgt tatttattga
    54541 tgccgactta cgccgtggtt attcgcataa cctgtttacc gtgagtaatg aacatggctt
    54601 gtcggaatat ctggcaggta aagatgagct caacaaagtg atccagcatt ttggcaaagg
    54661 aggctttgat gtgattactc gcggtcaggt gccacctaac ccatctgaac tgctgatgcg
    54721 cgatcggatg cgtcaattac tggaatgggc gaacgaccat tacgatctgg tgattgttga
    54781 tacgccgccg atgctggcgg tgagcgatgc tgcggtcgtg gggcgctctg tcggcaccag
    54841 cctgctggtt gcgcgttttg gcttgaacac cgccaaagag gtgagcttgt caatgcagcg
    54901 tctggaacag gctggcgtta atattaaagg cgcaatcctc aatggtgtga ttaaacgcgc
    54961 cagcaccgct tacagttacg gctataacta ttacggttat agttactccg agaaagagta
    55021 aaactgaagg taaagtgacc ggatgcggcg tgaacgcctt ttccggtcta cgcattagac
    55081 agttcttcgt ttgtcatcag cctcagagca ttcaggtagc tgaatgctct tttttataca
    55141 ttacaaactg cacgccggta tgcgtgcttc attcacgatt tgcgtaaaac cggccaacga
    55201 acacatgccc tgcgcatttc gctcttcaca tcctgccagg gtcagtttca cctctccggg
    55261 cggcgtattt aatgacagcg gagttttatc acgcatctgc tgtaaagtct ggaagaccag
    55321 cgaaacctga atccactggc tgttatcgct tagccgacgc cagcgttcaa acaccagttc
    55381 accacctggc ggcgtgttat ccggctgacc tggaagcgtc cagttgagct ccagtgcgcc
    55441 gccgagattt gccagattag tatcgtgtcc ggcgataaac agcactgaag tgggtaatgt
    55501 cacaccatac gcctgttttt gcggtggatg gggagtcaac gctatcatga tcaaatccaa
    55561 caacggggtg gcgcggctgc gggcaacctc tggcgtgcgt tgtagtaaat aaaattgcgc
    55621 gttatgcaaa cttagcaacg tgttccactg gtgtgaatca gtgatccttc cccaccccgg
    55681 ctccggcatt ccctgtgcct gttgcaggag aaatatttcc gtcagcattg atgcgaggct
    55741 taccgcaccg gttaatgaga cattgtcggc gctcaccttg agttccgatg gtaatgcctg
    55801 cgttaatgaa cagctttcgt cctgtttctc acggttaagg cacaagtttg attgcggaaa
    55861 attaagcacc cgttccagtt cgcgaaacgc cgtttgccga tgcccggtaa agtcagcaat
    55921 tgaccctcct gccctgctga ggatcgcgtc agtcacgttc gcgttatcca gttggcaaac
    55981 gccagttttt agaggattaa ataacggatc gggactggac gtatctgcct gggtatgtac
    56041 ggttattgca cagtcaggtg ccagcccggc ggcgaaggct tcgcctgttt tacgggtacg
    56101 ctcgtcgaca tcagcaataa tcgcgacctg accaggctgc gggcagccct ttttcgtcag
    56161 caatccgtcg gctaccagac gctggcgttg gtaatgtccg agataagcga ttagctcacc
    56221 accgcgcggt gtcagccaac ccagttttac cggccagttt ggccatgcgt ctggggtgac
    56281 atcctgcatc agttgcgtgg ccttggttgg ggcacgcaca ccatgacgac tgacaatcac
    56341 cacactttcc agcttcagct ccggctccgg ctcactctga gcgaatgcag attgcggggt
    56401 taacggaatc agaagagata aaaatgggat caagatcgct ttcatcgata tgtttccgct
    56461 tttcatatcc gacattattg attgcctgat acgacgctaa tgcgccgcat cagacttacg
    56521 ttcgttaact tttcaatcgc gggtccagcc ataccaacac aagggtggag agcgaacaca
    56581 tcaacaaaat gccgacgaac caaagtaaat accacattgt tcctgctcct tagtacagct
    56641 cgttttcgtt acggcggaga gtttctgtcg tcatgcgccc ccacattttg tagtagctcc
    56701 agagagtgta gagcaacaca atgggcaaaa atatcagcac gattaccaac ataatactca
    56761 gcgtcagctg actggaagtg ctgtcccaca acgtcaggct ggagattgga ctcacgcttg
    56821 acggcatgac aaaggggaac agcgtgatgc ctgccgtgaa aatcacgcca aattgcatca
    56881 atgacgccat caaaaatccc caacccgggc ggccacgata aatcgccatc accgtcagca
    56941 atgggcagaa gaaccccagc agcgggaaga tccacaaaac gggcgactcg acaaaattat
    57001 tcatccaggc accaggtagc actgccacca gtttcattaa cggattggaa ggaccgttag
    57061 catcctgggc gagcagtaca aagccatcaa taccgaccca cagccagtaa cccgccagca
    57121 aaaagcacag catcaccaac agtgcagcgc gtttagtcga cagctgtgaa cgcagatgaa
    57181 tcacaccaac agttttcagt tgtaaccaga cgccaccttg caaaatcacc atcccaaggc
    57241 tgagcaatcc gcacaataaa gggaatggcg tcagcagttg ccagaagctg ccgagatact
    57301 ccacgcgtaa ttgcggagtg aaggcaaacg gcacgccgag caacaagttg ccgaaggcga
    57361 taccgaagac taccggcggc accagactgc cgatgaccag accggcatcc cacattttac
    57421 gccagcgtgc atcggcgatt tttccgcgat aatcaaaggc cagcgggcgg aagaacagcg
    57481 agcacagcac caggatcatc gccacataaa agccggaaaa cgccgctgca tacactctgg
    57541 gccaggcggc aaataatgcc ccacctgcga ggatcaacca gacctgattg ccttcccagt
    57601 gcgcaccaac gctattaatc actatccggc gttcatcatc atcacgcgcc accagcggca
    57661 gcaaacagcc aatccccatg tcaaatccgt cggagatcat aaagaccacc aggatcacgc
    57721 caatcagcag ccaccagatg aagcgcaatg tttcataatc aaacatgatt ttctccttta
    57781 cccctgttgc tgcgtcggtt gttcactctg catcgcgctc ggccccagac gggcatattt
    57841 ctgcatcagg tagacttcgg cgattaagaa cagggtgtaa agccctacga tcatgatcag
    57901 tgagaaagcc agttgtcctg tggttaaagc ggagtgcgcg gagtatgtcg gtaagatgtc
    57961 ctgtatcgcc cacggctgac gaccaaactc ggtcataaac cacccggctt caatcgcaat
    58021 ccacggcaac ggcaaactcc agagcgccat tttcagcacc cagcgatgct ggtcgatttt
    58081 gccacgcagc gtctggacaa gcgcaatcag catcaccagt agcagcaggg aaccacagcc
    58141 caccatgatg cggaaactcc agaataccgg cgcaacctga ggtatcgcgc cacgcatcgc
    58201 cgcctggtac tgtgcggctg tgacatgatt catatccggc gcatagcggg agagcaacat
    58261 gccgtagccc aggtcgcctt ccagctcccg gaatgcctgc aacacatgcg gctcacgatt
    58321 gccttgcgat atttcctgca ttaacagcca ggccatacgt ccgcgttgca agcgtgggta
    58381 ggtttcagcc atcaaattct tcagacccgg cacgggttta tctaatgagt gagtggcgag
    58441 gatccctaac agcacgggaa ttttgatggc aaaggcgtta cgctcttgat cctgttccgg
    58501 ccaggcaacc acatggaacg gtgcaggagc aggttccgtt tgccactccc cttccatcgc
    58561 cgccagtttt accggttgta cttgcgcgac ttcatacgca gaactgtctc cgagttgcag
    58621 ggtaccgata atcgccagag taccgaagac ggaaccgatg gcaaacgagc gtaatgcgac
    58681 atcgcgctcc cgtccgcgca gtaaatacca ggcgctgatc gccataataa acatggcccc
    58741 ggtcacgtag cccgccatta cggtgtgcac aaatttcacc tggctgaccg ggttaaagac
    58801 cagctcgctg aagctggtca tctccatacg cagggtgtcg atatcaaaat gcgcaccggt
    58861 cgggtattgc atccaaccgt tggcattcaa tatccacaat gcagagagat ttgaaccgaa
    58921 cgccaccagc cacgtcacca gcaggtgctg gtatttattc agccgctgcc agccgaagaa
    58981 gaaaagcccg acaaaggtgg attcgaggaa gaaggccatt aatgcttcca tcgccagcgg
    59041 tgcgccaaaa atatcgccca catagttgga atagaatgac cagttagtac caaactgaaa
    59101 ctccatggtc aggccggtag ccacgccaag agcaaagttg ataccgaaga gcttacccca
    59161 gaagcgcgtc atatcgcggt agattgtttt gccggtgacc acgtaaatgg tttccatgat
    59221 agccagcaaa aaaatcagcc ccagggtaag gggtacaaat aaaaagtgat acagcgcggt
    59281 cagagcaaac tgccagcgcg ataaatcaat gacatcccac atgcgcactc ctgtaggcga
    59341 agagagaaaa ataacccgtt aatggtagat ctccctcttt atcctgaaac tctacgacca
    59401 gaggtattcg ccgattgcga tattcataag cctgaaagcg atacttaccg tctttttacg
    59461 tcggtgcagc ttccgccagc cactgacata cctcactaag ccgctgcgca gagtcgacca
    59521 aatcttctgg cgctgccagc accacttccg gtattgggca gatttcataa ctttccagta
    59581 acgggccttt taaggtgtcc gtacagcgta aatgccagac atggcgtaac cccgtggcgt
    59641 tgatatagct ctcgccatag ccaatggtac gaatctgaat atttcccggc ccacagagac
    59701 gtgagagaaa aagccgatca gcctcgctga tgggtaattg cgtcagatta atgctgtgcg
    59761 gctgcgcgtc ggggttacgt acatgcgcca gtaactcatg cgccaacggt aggccattca
    59821 tcaggccatc aatgggcggc ggtaacagcg aatctgtcgg caagagattt tgcgttgccg
    59881 cctgccacaa cgccagcggc gcgcagccag cctccagttt gtcctccagc aacttttcgc
    59941 cgcgacgtct gcgcacccgc cataatccgc agaagatcgc ctcctgtatt tcactttcgc
    60001 tgtcgtcagc ctgctgaatg cgcactgaca cttcaccttc cccgagaagc gtattgataa
    60061 agtgacgatc gtcggcgttg agatttaaca gatcgactgc cagcacctcg cccaacggcg
    60121 gctggcgttg acttagttga tgttgtaact cgtttaacag cgcaatcacc tgcgggctgt
    60181 gagcacattg ctccagggcc gccatactcg gttcatcatt cacctgacag gtgatcggca
    60241 gtggattcat gctgaaactg tcatcgttcg gttgcgttct tggccccagc agatggaaaa
    60301 aagtttcgct catgaggcac gctcctgctg tggttcgaca agcccacgca tcaggtttat
    60361 cagttccgcc cacgggtgaa taccattcag cacgccgcga tagtttccgc cggtaaacac
    60421 taatgtggcg ggaaagcgaa agacgccaaa gcgatctccg atggcttcgc tctgttcaag
    60481 gtcagcaatc gccacctgcc atgtgtagtc gggaaactcg cgcagtaatt cgccaatcat
    60541 taccggatta tcgctgacct ctggcgtgcg tttcgggtca ctgcttaata acaccacgcc
    60601 gtctggcgct tgcgtaagcc agtcgtcaag acggaattca ctaaccggcg tccagccgcg
    60661 cgccagcatt cgttgccaca acgcatcaaa tggcgtgtcg ttgctcatcc ctgctcctcc
    60721 agtgtcatcc tgtactggcg caagcgaacg ccttcgtaat tttccatcga cagacagtca
    60781 taattgagac agcgcgattc attagccggt tgcggcacaa ttccccatgc agccagctgc
    60841 gccagcgccg cctgttccgc tgcgggcagt tgctcccgtg ccagttcgct caggctgccg
    60901 ccgtagtcgt cgagcattgc gggttgcaga ccgacgaggg caatatgtgc tggcagatgt
    60961 ccgcggatat ctgccagcgc cagcacttcg gagaaactgt tctggtgcag gctcattttc
    61021 ttcgcgctga gataagccgg aatacgttct ccggcatagg ttcgcagcgt tcctggttcc
    61081 agcccgtagt caatggcatc gagaatcaac agatggctgg cgctttcgac ataccccagc
    61141 aagttcagtc cctgagtacc gccatcgaca atctccacag actcgggcca gtggtaatgg
    61201 gcatacaacc gttccgccac ccgcacgccg aaaccttcat cggcccacag caggttgccc
    61261 agccccatta ccaccacgcg ttgctcgctc atgaacgctc cttgttactt attttgccaa
    61321 atttgtggct acggtagccg ttgaccatgg tggagatcac cgtgtcgtcg gacatgatgt
    61381 cttcacgcag cgccatatag acatgaccga tcacaaacgc gccaatcagc cacatcccca
    61441 gccgatgcca gctgtgaatg tccattgagt tgcctcccgt ccagtagaaa aattccacca
    61501 cataacggaa cggcgcaaaa atagcgtact ggctgtgttc gctgtacagc gcaaagccgg
    61561 tgatgatcat aaagaccgac atcaggaaat agccaaacat cgccgcctgg gcgatggggt
    61621 tatggcctat atcggcactc ggacgttttg ccagaaacag ataccagcgg atttcatacc
    61681 acacgccctg ccaccagctt ttacgccata ctggcacgat aaacagctcg cgggagtatc
    61741 gattgccaac aaaagcccag tagatccgca tcagcaagac cacggtaaaa accatcccgg
    61801 cgctgaagtg aattaacctg atgtagccca tatagaacag atacgtcgcc tcgccgctga
    61861 cggaaggtag cggtttgccg ataaagtaac cggtgaccat caacaccgcc atgcataaca
    61921 ccgtcagcca gtgccagata cgcactggcg cttcaaagac atagtggctg acaacgttgt
    61981 cgcttttctg ttgcatgatg attctccttc gctgttaacg cacctgcacg gagatcagct
    62041 cgctaccgtc gtcgcccagc acgtgtgttg aacaggcgag gcacggatca aagctgtgca
    62101 gagtacgcag gatctccagc ggttgctcgg ggatcgccat tttggtgttc atcagcgccg
    62161 cttcataagc gccaatctgc cctttgggat cgcgcgggct ggcgttccag gtggtcggca
    62221 ccacgcactg gtagagatca atcttgccat cgcgaatggc ggcccagtgg cctaacgccc
    62281 cgcgcggcgc ttcggtaaaa ccgacaccac ggcactctgt cggccaggtt gtgggttccc
    62341 atttttccgt ggaggcggtg gcaagattgc cgtttttcag gttagtcatc agcttgttga
    62401 agaaatactg caacttacct gcggcccact gtgcttcgtg cgcgcggcac aaaatgcggc
    62461 ccaacgttga ctggataccg gaaagcggca ggttcagcgc cgacatcatg cgatcgaccg
    62521 actcaacggt cgcagcatcg cctttgtgat aagcgattaa cgtgcgtgcc agcggcccca
    62581 cttccatcgc gttaccgcgc cagcgcggcg ctttgatcca cgagtagcgt tcctgttcat
    62641 tcagctgctg aatgttggta tcgctgcctt tgacatcgcc ggggttgtac cacgggtcgg
    62701 tgatgccatc gaacggatga cgcccgacct gatcgttggg ataacgatac caggcgtgat
    62761 cgacaaactc ctgcacctgc tgcggatcaa ccaaatccac tggcagcaca ttgttgaagt
    62821 cgccgttaat caccgcgccg ccaggcatca gcagactttt ctcaccaaag tcgttggcaa
    62881 tatccgggaa tgcgccgtag ctgaggacac atttatcaga aagacccgtg ccgatttcgc
    62941 tccacggttt gttgaactga ccgatggcta aggcgtcggg gatcatcacg ttgttaatga
    63001 agtctgccgt gcgggtgata atcgactgca ccaggttcag gcgttccata ttgaccgccc
    63061 cgaccgcgcc gctttcgtca atgttaatgg cgcaaggcat cccgccgaca atccagtttg
    63121 gatgcgggtt tttaccgcca aagaccgcgt ggattttgac aatttcacgc tggaaatcga
    63181 gagcttcgag atagtgggca aagcccatca ggttggcttc tggcggcaac ttgtactgcg
    63241 ggtgccccca gtagccattg cggaagatcc ccaactgccc gccttcaacg aatttcttca
    63301 ggcggttttg tacgtcgaag aaatagccag gggatgattt cggccaagag gagagacttt
    63361 gcgccagttc ggaggttttc cgcgggtcgg ctttcagcgc atccaacaca tcgatccagt
    63421 ccatcccggc aagctgatag aagtgcacca gatgatcgtg gcaccagagc gttgccagca
    63481 taatgttgcg gatgatattg gcgttgtccg gcactttaat accgatggca tcttcgatgg
    63541 cgtaaaccga agccagagcg tgtacgccag tacagacgcc gcagatacgt tcaacgaacg
    63601 cccacgcatc gcgcgggtcg cgcccttgca ggatgatctc cagcccgcga aacatggtgc
    63661 cgcaggagac ggcattggtg attacattct gatcgttaat attcacttcg cagcgcatgt
    63721 ggccttcaat gcgcgtaatc gggtcgacca ccaggcggcg tccggcatta ttgatggtgt
    63781 atccctgagt ttcgtactga gtgctcatgc ctgtttatcc tcattgcctg gctgatgttc
    63841 ggtttctgta ggttgctgat tatgacgtct gcgctggtca acggcgctgg cgactgcgtg
    63901 cacaccaaca gccgctgcca ccacaccaag cgcggttaaa ccgacggtat cggcggtgga
    63961 atgagtaccc atttgtggaa tatcgaccac gcggctgtag aacgaaccgc gatcccagaa
    64021 accattttcc gcacagccca ggcagccgtg accagactgg attgggaaag aaacgccatc
    64081 attccagcgt gtggaggaac aggcgttata ggtggtaggc cctttgcagc ccattttgta
    64141 caggcaataa cctttgcggg cagcgtcatc atcccaactc tggacgaact ctccggcgtc
    64201 gaagtgagcg cggcgatagc atttatcgtg gatacgctga ccataaaaca tcagtggacg
    64261 acccattctg tcgacatctg gcaagcgatc aaaggtcacc atgtaagtaa tgatggcgct
    64321 catcacatcc gggatcggcg ggcagccagg tactttgata atgggtttgt cggtgatgac
    64381 tttgtcgata gacgttgcct gtgtcggatt aggtcgcgcg gcctgcacgc agccccagga
    64441 cgcacaggtt ccccaggcga taatcgcgct ggctccggca gcggcacgtt tgagtttctc
    64501 aataaacggt cgaccgctgc tgatacagaa catcccctgc tcgcccagcg gcggattacc
    64561 ttctactgcg aggatatatt tgccattgta ttgcgtgatg atgtcttcaa agacttcttc
    64621 cgcctgggtt ccggcggcag ccatcaaagt atcgtcgtaa tcgagggaaa tcagggaaag
    64681 gatgacgtcc tttgccagcg ggtgagcgga gcggataaaa gattcggtac agcaggtgca
    64741 ttccagaccg tggatccata ccaccggaat gcgcggtttg ttctccagcg cccaggcaat
    64801 ctttggtgcc attcccgcgc ctaatcccag cgacgtggca gccagactac aatatttgag
    64861 aaagctgcgc cgggtaacgc cctgacgccg catggcctgg taaaatgttt cctcgttatt
    64921 catatcgcac gtctcttctc cttgcgacac cggcaggaca ccgggcgctt atggtcggca
    64981 agcgacgtcc ctgtagcaag gcaaaccatc gcttcacgtg agatgctgaa aacgaaagct
    65041 catccttctg cactggcgca cgtcgccaga aagtattgtt aataaagcgt agtgaaactt
    65101 ttgcacaaaa caatacaaac tgtgtggatt tatcttttag cgataaaaat ggacctattt
    65161 ttctttttgc cgggcggtgg gatgtttagc cagttgctaa acgagtaaaa gagaaggaat
    65221 ttatggtgat gaaatgatca atctgctttt atttcacgct atcaatgcac ttttggctgt
    65281 ttttcaggca aacaaacaga ttaggggttt acacgccgca tcgggatgtt tatagtgcgc
    65341 gtcattccgg aagtgtggcc gagcggttga aggcaccggt cttgaaaacc ggcgacccga
    65401 aagggttcca gagttcgaat ctctgcgctt ccgccaaata atcaaggggt taccaaatgg
    65461 tagccccttt gcttttttgg ctcttggtat aaacttggta taccaattgg tatattttta
    65521 ttcggatgag attatcggtc atgagcggaa cctcaggttc ctcgtctgat gccgctcttg
    65581 caacacgtta cgctgccgag tatttttgta aaacatggac tgctccaggc ctgaaccagg
    65641 cagaaggata caaagccatc tcagatctgt cgcatcacta ttttcgtgca gaaggctcta
    65701 gccctcccca atcttttttg tgacaccctg tttcactgaa catgaagaca ctcaccttgt
    65761 ccctgatatt cattagcatc atcctgatta tttaacttct ctatttaggg aagcccgtct
    65821 gatacaaagc ttctattcct cgttaaatat tctccattct ggcgccaagc cctatctata
    65881 gctgattgaa aacaactcta aaaacgtaat aatatcaaat agtttattcc tacagccgat
    65941 cattttgagt aaaatattga cgagaatttc acaaaaggta gatttcatgt aaacggataa
    66001 ataagactat ccaataaagc tcttataagg ttatctatat gataaatagc attaattctt
    66061 tttttccggc cttcctcgca gtatatcaag tgctatacgc agcagtacct tcactgtctc
    66121 gcagcacaaa agcacaccta acacggtaaa aaccagctca cctttttctc ccagcaacag
    66181 tcctgccagt gcaacaacta tttttaaggt aaaaaattct tatacagaat caggacttca
    66241 acgtcccaca tcttatactc agtcaagtat agaaaaaaac gctttacatc ggcctctacc
    66301 tgatgtagct cagcgtctgg tgcagcatct tgcagagcat ggcattcaac cagcccggag
    66361 tatggctgaa catattcctc cggcacctaa ctggcctgcg ccaccaccgc cagtacaaaa
    66421 tgaacaatca agacct
//
LOCUS       DAZFBR010000011        65227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFBR010000011 DAZFBR010000000
VERSION     DAZFBR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.011"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            60..692
                     /gene="comR"
                     /locus_tag="ACHJZ2_000872"
     CDS             60..692
                     /gene="comR"
                     /locus_tag="ACHJZ2_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO4567037.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(754..1293)
                     /gene="ycfJ"
                     /locus_tag="ACHJZ2_000873"
     CDS             complement(754..1293)
                     /gene="ycfJ"
                     /locus_tag="ACHJZ2_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4567038.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1520..2824)
                     /gene="ndh"
                     /locus_tag="ACHJZ2_000874"
     CDS             complement(1520..2824)
                     /gene="ndh"
                     /locus_tag="ACHJZ2_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO4567039.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3224..3766)
                     /gene="ycfP"
                     /locus_tag="ACHJZ2_000875"
     CDS             complement(3224..3766)
                     /gene="ycfP"
                     /locus_tag="ACHJZ2_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO4567040.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3789..4814)
                     /gene="nagZ"
                     /locus_tag="ACHJZ2_000876"
     CDS             complement(3789..4814)
                     /gene="nagZ"
                     /locus_tag="ACHJZ2_000876"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO4567041.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4825..5649)
                     /gene="thiK"
                     /locus_tag="ACHJZ2_000877"
     CDS             complement(4825..5649)
                     /gene="thiK"
                     /locus_tag="ACHJZ2_000877"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO4567042.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5630..6271)
                     /gene="lpoB"
                     /locus_tag="ACHJZ2_000878"
     CDS             complement(5630..6271)
                     /gene="lpoB"
                     /locus_tag="ACHJZ2_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO4567043.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6285..6662)
                     /gene="ycfL"
                     /locus_tag="ACHJZ2_000879"
     CDS             complement(6285..6662)
                     /gene="ycfL"
                     /locus_tag="ACHJZ2_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO4567044.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6665..7024)
                     /gene="hinT"
                     /locus_tag="ACHJZ2_000880"
     CDS             complement(6665..7024)
                     /gene="hinT"
                     /locus_tag="ACHJZ2_000880"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO4567045.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7364..9553
                     /gene="fhuE"
                     /locus_tag="ACHJZ2_000881"
     CDS             7364..9553
                     /gene="fhuE"
                     /locus_tag="ACHJZ2_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO4567046.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9613..11046)
                     /gene="ptsG"
                     /locus_tag="ACHJZ2_000882"
     CDS             complement(9613..11046)
                     /gene="ptsG"
                     /locus_tag="ACHJZ2_000882"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO4567047.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11341..12138)
                     /gene="ycfH"
                     /locus_tag="ACHJZ2_000883"
     CDS             complement(11341..12138)
                     /gene="ycfH"
                     /locus_tag="ACHJZ2_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4567048.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12149..13153)
                     /gene="holB"
                     /locus_tag="ACHJZ2_000884"
     CDS             complement(12149..13153)
                     /gene="holB"
                     /locus_tag="ACHJZ2_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO4567049.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13150..13791)
                     /gene="tmk"
                     /locus_tag="ACHJZ2_000885"
     CDS             complement(13150..13791)
                     /gene="tmk"
                     /locus_tag="ACHJZ2_000885"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4567050.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(13781..14803)
                     /gene="yceG"
                     /locus_tag="ACHJZ2_000886"
     CDS             complement(13781..14803)
                     /gene="yceG"
                     /locus_tag="ACHJZ2_000886"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO4567051.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14806..15615)
                     /gene="pabC"
                     /locus_tag="ACHJZ2_000887"
     CDS             complement(14806..15615)
                     /gene="pabC"
                     /locus_tag="ACHJZ2_000887"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO4567052.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15735..16976)
                     /gene="fabF"
                     /locus_tag="ACHJZ2_000888"
     CDS             complement(15735..16976)
                     /gene="fabF"
                     /locus_tag="ACHJZ2_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO4567053.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17064..17300)
                     /gene="acpP"
                     /locus_tag="ACHJZ2_000889"
     CDS             complement(17064..17300)
                     /gene="acpP"
                     /locus_tag="ACHJZ2_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4567054.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17511..18245)
                     /gene="fabG"
                     /locus_tag="ACHJZ2_000890"
     CDS             complement(17511..18245)
                     /gene="fabG"
                     /locus_tag="ACHJZ2_000890"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO4567055.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18258..19187)
                     /gene="fabD"
                     /locus_tag="ACHJZ2_000891"
     CDS             complement(18258..19187)
                     /gene="fabD"
                     /locus_tag="ACHJZ2_000891"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO4567056.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19203..20156)
                     /gene="fabH"
                     /locus_tag="ACHJZ2_000892"
     CDS             complement(19203..20156)
                     /gene="fabH"
                     /locus_tag="ACHJZ2_000892"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO4567057.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20224..21294)
                     /gene="plsX"
                     /locus_tag="ACHJZ2_000893"
     CDS             complement(20224..21294)
                     /gene="plsX"
                     /locus_tag="ACHJZ2_000893"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO4567058.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21375..21548)
                     /gene="rpmF"
                     /locus_tag="ACHJZ2_000894"
     CDS             complement(21375..21548)
                     /gene="rpmF"
                     /locus_tag="ACHJZ2_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO4567059.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21600..22121)
                     /gene="yceD"
                     /locus_tag="ACHJZ2_000895"
     CDS             complement(21600..22121)
                     /gene="yceD"
                     /locus_tag="ACHJZ2_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO4567060.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22320..22904
                     /gene="yceF"
                     /locus_tag="ACHJZ2_000896"
     CDS             22320..22904
                     /gene="yceF"
                     /locus_tag="ACHJZ2_000896"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO4567061.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23005..23964)
                     /gene="rluC"
                     /locus_tag="ACHJZ2_000897"
     CDS             complement(23005..23964)
                     /gene="rluC"
                     /locus_tag="ACHJZ2_000897"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO4567062.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24537..27722
                     /gene="rne"
                     /locus_tag="ACHJZ2_000898"
     CDS             24537..27722
                     /gene="rne"
                     /locus_tag="ACHJZ2_000898"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO4567063.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27917..28870)
                     /gene="flgL"
                     /locus_tag="ACHJZ2_000899"
     CDS             complement(27917..28870)
                     /gene="flgL"
                     /locus_tag="ACHJZ2_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO4567064.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28882..30525)
                     /gene="flgK"
                     /locus_tag="ACHJZ2_000900"
     CDS             complement(28882..30525)
                     /gene="flgK"
                     /locus_tag="ACHJZ2_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO4567065.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30591..31532)
                     /gene="flgJ"
                     /locus_tag="ACHJZ2_000901"
     CDS             complement(30591..31532)
                     /gene="flgJ"
                     /locus_tag="ACHJZ2_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO4567066.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31532..32629)
                     /gene="flgI"
                     /locus_tag="ACHJZ2_000902"
     CDS             complement(31532..32629)
                     /gene="flgI"
                     /locus_tag="ACHJZ2_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO4567067.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32641..33339)
                     /gene="flgH"
                     /locus_tag="ACHJZ2_000903"
     CDS             complement(32641..33339)
                     /gene="flgH"
                     /locus_tag="ACHJZ2_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO4567068.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33392..34174)
                     /gene="flgG"
                     /locus_tag="ACHJZ2_000904"
     CDS             complement(33392..34174)
                     /gene="flgG"
                     /locus_tag="ACHJZ2_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO4567069.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34312..35067)
                     /gene="flgF"
                     /locus_tag="ACHJZ2_000905"
     CDS             complement(34312..35067)
                     /gene="flgF"
                     /locus_tag="ACHJZ2_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO4567070.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35087..36292)
                     /gene="flgE"
                     /locus_tag="ACHJZ2_000906"
     CDS             complement(35087..36292)
                     /gene="flgE"
                     /locus_tag="ACHJZ2_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO4567071.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36317..37012)
                     /gene="flgD"
                     /locus_tag="ACHJZ2_000907"
     CDS             complement(36317..37012)
                     /gene="flgD"
                     /locus_tag="ACHJZ2_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO4567072.1"
                     /translation="MSIVVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37024..37428)
                     /gene="flgC"
                     /locus_tag="ACHJZ2_000908"
     CDS             complement(37024..37428)
                     /gene="flgC"
                     /locus_tag="ACHJZ2_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO4567073.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37432..37848)
                     /gene="flgB"
                     /locus_tag="ACHJZ2_000909"
     CDS             complement(37432..37848)
                     /gene="flgB"
                     /locus_tag="ACHJZ2_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO4567074.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38003..38662
                     /gene="flgA"
                     /locus_tag="ACHJZ2_000910"
     CDS             38003..38662
                     /gene="flgA"
                     /locus_tag="ACHJZ2_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO4567075.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38738..39031
                     /gene="flgM"
                     /locus_tag="ACHJZ2_000911"
     CDS             38738..39031
                     /gene="flgM"
                     /locus_tag="ACHJZ2_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO4567076.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39036..39452
                     /gene="flgN"
                     /locus_tag="ACHJZ2_000912"
     CDS             39036..39452
                     /gene="flgN"
                     /locus_tag="ACHJZ2_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO4567077.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39492..41027)
                     /gene="murJ"
                     /locus_tag="ACHJZ2_000913"
     CDS             complement(39492..41027)
                     /gene="murJ"
                     /locus_tag="ACHJZ2_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO4567078.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41137..42060)
                     /gene="yceM"
                     /locus_tag="ACHJZ2_000914"
     CDS             complement(41137..42060)
                     /gene="yceM"
                     /locus_tag="ACHJZ2_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4567079.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42062..42709)
                     /gene="yceH"
                     /locus_tag="ACHJZ2_000915"
     CDS             complement(42062..42709)
                     /gene="yceH"
                     /locus_tag="ACHJZ2_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO4567080.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42720..43304)
                     /gene="rimJ"
                     /locus_tag="ACHJZ2_000916"
     CDS             complement(42720..43304)
                     /gene="rimJ"
                     /locus_tag="ACHJZ2_000916"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4567081.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43540..44748
                     /gene="mdtH"
                     /locus_tag="ACHJZ2_000917"
     CDS             43540..44748
                     /gene="mdtH"
                     /locus_tag="ACHJZ2_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO4567082.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44812..45459
                     /gene="grxB"
                     /locus_tag="ACHJZ2_000918"
     CDS             44812..45459
                     /gene="grxB"
                     /locus_tag="ACHJZ2_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO4567083.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45593..46153
                     /gene="yceB"
                     /locus_tag="ACHJZ2_000919"
     CDS             45593..46153
                     /gene="yceB"
                     /locus_tag="ACHJZ2_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4567084.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46259..47305
                     /gene="pyrC"
                     /locus_tag="ACHJZ2_000920"
     CDS             46259..47305
                     /gene="pyrC"
                     /locus_tag="ACHJZ2_000920"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO4567085.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPARHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47379..47624
                     /gene="dinI"
                     /locus_tag="ACHJZ2_000921"
     CDS             47379..47624
                     /gene="dinI"
                     /locus_tag="ACHJZ2_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO4567086.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47914..48168
                     /gene="bssS"
                     /locus_tag="ACHJZ2_000922"
     CDS             47914..48168
                     /gene="bssS"
                     /locus_tag="ACHJZ2_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO4567087.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48283..49401
                     /gene="solA"
                     /locus_tag="ACHJZ2_000923"
     CDS             48283..49401
                     /gene="solA"
                     /locus_tag="ACHJZ2_000923"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO4567088.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            49449..49562
                     /locus_tag="ACHJZ2_000924"
     CDS             49449..49562
                     /locus_tag="ACHJZ2_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO4567089.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49823..50389
                     /gene="yceJ"
                     /locus_tag="ACHJZ2_000925"
     CDS             49823..50389
                     /gene="yceJ"
                     /locus_tag="ACHJZ2_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO4567090.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50393..50968
                     /gene="yceI"
                     /locus_tag="ACHJZ2_000926"
     CDS             50393..50968
                     /gene="yceI"
                     /locus_tag="ACHJZ2_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO4567091.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(51010..52062)
                     /gene="trhO"
                     /locus_tag="ACHJZ2_000927"
     CDS             complement(51010..52062)
                     /gene="trhO"
                     /locus_tag="ACHJZ2_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO4567092.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52287..53207
                     /gene="lpxL"
                     /locus_tag="ACHJZ2_000928"
     CDS             52287..53207
                     /gene="lpxL"
                     /locus_tag="ACHJZ2_000928"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO4567093.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53379..54605
                     /gene="mdtG"
                     /locus_tag="ACHJZ2_000929"
                     /pseudo
     CDS             53379..54605
                     /gene="mdtG"
                     /locus_tag="ACHJZ2_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            54688..55062
                     /gene="msyB"
                     /locus_tag="ACHJZ2_000930"
     CDS             54688..55062
                     /gene="msyB"
                     /locus_tag="ACHJZ2_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4567094.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(55063..55290)
                     /gene="yceK"
                     /locus_tag="ACHJZ2_000931"
     CDS             complement(55063..55290)
                     /gene="yceK"
                     /locus_tag="ACHJZ2_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4567095.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(55463..58006)
                     /gene="mdoH"
                     /locus_tag="ACHJZ2_000932"
     CDS             complement(55463..58006)
                     /gene="mdoH"
                     /locus_tag="ACHJZ2_000932"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO4567096.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(57999..59534)
                     /gene="mdoG"
                     /locus_tag="ACHJZ2_000933"
     CDS             complement(57999..59534)
                     /gene="mdoG"
                     /locus_tag="ACHJZ2_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO4567097.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            59927..61084
                     /gene="mdoC"
                     /locus_tag="ACHJZ2_000934"
     CDS             59927..61084
                     /gene="mdoC"
                     /locus_tag="ACHJZ2_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO4567098.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGTFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(61092..62573)
                     /gene="clsC"
                     /locus_tag="ACHJZ2_000935"
     CDS             complement(61092..62573)
                     /gene="clsC"
                     /locus_tag="ACHJZ2_000935"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO4567099.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(62515..63048)
                     /gene="ymdB"
                     /locus_tag="ACHJZ2_000936"
     CDS             complement(62515..63048)
                     /gene="ymdB"
                     /locus_tag="ACHJZ2_000936"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO4567100.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(63143..63454)
                     /gene="ymdA"
                     /locus_tag="ACHJZ2_000937"
     CDS             complement(63143..63454)
                     /gene="ymdA"
                     /locus_tag="ACHJZ2_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO4567101.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(63575..63907)
                     /gene="csgC"
                     /locus_tag="ACHJZ2_000938"
     CDS             complement(63575..63907)
                     /gene="csgC"
                     /locus_tag="ACHJZ2_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO4567102.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(63966..64424)
                     /gene="csgA"
                     /locus_tag="ACHJZ2_000939"
     CDS             complement(63966..64424)
                     /gene="csgA"
                     /locus_tag="ACHJZ2_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO4567103.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(64465..64920)
                     /gene="csgB"
                     /locus_tag="ACHJZ2_000940"
     CDS             complement(64465..64920)
                     /gene="csgB"
                     /locus_tag="ACHJZ2_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO4567104.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT        15915 a        17327 c        16010 g        15975 t
ORIGIN      
        1 acggaggtga ttatttttat aataaccttt cattcacagt caccaggtac aacggcatca
       61 tggcaactga ctcaacacaa tgtgtaaaaa aaagccgtgg ccgcccaaaa gtgttcgaca
      121 gggatgccgc gcttgataag gccatgaaat tgttctggca acacggttat gaagcgactt
      181 ctcttgcgga cctcgtcgaa gcgaccggag ccaaagcgcc cacgctatac gcggaattta
      241 ccaacaaaga ggggttattt cgtgccgttc tcgaccgcta tatcgatcgt tttgccgcta
      301 agcatgaagc acagctgttt tgtgaagaga aaagcgtgga gtctgcgctg gctgactatt
      361 ttgctgccat cgccaactgc tttaccggca aagacacccc ggcaggctgc ttcatgatca
      421 acaactgcac caccctctcc ccagattcag gagatatcgc caatacgttg aaatcacgcc
      481 atgcgatgca agagcgcact ttgcagcagt ttttatgtca acgacaagcg cgcggggaaa
      541 tcccgaccca ctgtgacgtg acacatctgg cagaattcct taattgtatt attcagggga
      601 tgtcgatcag cgcacgcgaa ggtgcatcgt tggaaaaact gatgcagatt gccggaacga
      661 ctttgcgttt atggcccgaa ctggtgaaat aagtcgcaaa aaaagcccct cagcctgagc
      721 gaatgagggg ccaaattgca gagtacagcc ttgttatact ttgttattca aaatcagttg
      781 cccgttacta tctagcggga tctgcgtacc cggatcgcgg tccatgcgga ttttgccctg
      841 ctgatcgcca atcttatagg tcacatcata accgagcatt ttttctgact tgtcatacac
      901 cgttttacaa cgctgttgcg tagtcgtgta agtatcgctt tcctggagag agccctggat
      961 ctggttaccg gcatatccac cacccagcgc ccccacaaca gtggcgacat ctttaccacg
     1021 accaccacca aactgatgcc ctatcacgcc gccagcaaca gcgccgagca ccgacccggt
     1081 aatgcgatgt tcatcctgca ccggtcgacg atgggtcact gtgacgttgc gacactcctg
     1141 acgcggtgtt ttaaccgttt ccttgattgg ggttgcagaa acaacctgag cgtattgcgg
     1201 gccacgttca aacacgttca gactggccac tgccgctacg ccaagcgcag ctgcgacacc
     1261 aatcccgata cccgccaaca ttgatttatt cacgggaata cctccttctt tgcagttatg
     1321 ccaatcatgc aagcgaagga gaaaagcagc tatcagacag aagatgaaaa gcgcgggaat
     1381 ggctggaaat acttaaggat tcggaggagt ctgaaggaat gcctgatgcg acgcttaacg
     1441 cgtcttatca ggcctaccgc tcgtaccatt tgtccagggc ggatgaggcg tttatgccac
     1501 atccgccagt gtacgtcgat taatgcaact tcaaacgcgg acggataacg cggttaatac
     1561 tccccaccag catcattaat ccggttttaa agtaaccatg cagcgcaatc tgatgcattc
     1621 ggtatagcga gatatataca aagcgcgcaa ttcgtccttc aatcatcatt gagccgcgcg
     1681 tcaggttacc catcaggcta ccaacggtgg agaagttcga cagcgatacc agcgaaccat
     1741 gatctttata ctgataattt ttcagcagct taccgttcat ctgcgccaga atgttgttca
     1801 ttgcgcaagt cgccatctgg tgtgcagcct gagcacgcgg cggaacaaag cccccttccg
     1861 gacgcgggca tgacgcgcag tcgccaatag cgtaaatgtc tggatcgcgg gtggtttgca
     1921 gcgtcagttc caccaccagc tggttgatac ggttagtttc aagaccaccg atatctttca
     1981 ggaagtctgg cgctttgatc ccggctgccc ataccatcag atcagcctca atatattcgc
     2041 catctttagt gtgcaggccg ccttcatcag cactggtgac catggtttgc gtcagaacgc
     2101 gaacgccaag tttcgttagc tcgttgtggg ccgcagcaga gatacgtggc ggtaatgcag
     2161 gcaaaatacg ttctcccgct tctaccagcg ttacgttcag ggcttcgttg gtcaggcctt
     2221 tgtaaccgta gctgtgcagt tgcttgaccg cgttgtgcaa ttcagcggag agttctacac
     2281 ccgtcgcgcc gccgccgaca atcgcaatgt tcactttgcc atttgcgccc aggttggcgg
     2341 agtatttcag gaagagattc agcatctcct ggtgaaagcg acgcgcctgg tgcgggttat
     2401 cgaggaaaat gcagttctct ttgacacctg gtgtattgaa atcgttagag gtgctaccca
     2461 gcgccattac cagggtgtca taggcgattt tacgttccgg aaccagcagt tcacctttct
     2521 catcgcgcag ttctgcaata gtgattgttt tcgcgtcacg atcaatatca atgacggaac
     2581 ccagctggaa ctggaaacca tgattgcgcg catgggccag atagctcaac gcatcgacgc
     2641 cttcatcaag cgagccagtc gctacttcgt gcagcagcgg tttccacagg tggctgtggt
     2701 tacgatcgac cagcgtaatt ttggcttttt tcttgcgtcc cagcttatgc cccagctgtg
     2761 ttgccatttc cagcccacca gcaccgccgc cgacaatcac aatttttttc aatggcgtag
     2821 tcaacgtgac ccccttaaat ttattaacca attgttaatt aatggttatt aacatagctc
     2881 ttaattaaca acaggttaca gcaatgaaaa tgttcttcgc tattgagaat aacatgaatg
     2941 gtgcattggt cataccaaaa ttgatgttaa tcaagttttg ttgctgaaaa gataggcgac
     3001 tttaccgggt gagatcaaaa aacagagttt attgtttatt atttgagcga atttgtgaga
     3061 gtgatatagc gtgaggggtg tatgtagggc gttcggtagg tctgataaga ggcgcatcag
     3121 gcattgatgc tcgactgtcg gatgcggcgt gaacgcctta tccgacttac agaattcgga
     3181 gcgcatcagg taccggcaag gttacctgat gcgacgaggg catttacccg agggttttga
     3241 acgctttaat gcgctgcaaa tgcggggaga tattcttgaa tttgtgcgtc tgctcttcgt
     3301 cccagacaat ctcgtaataa tgatgcaact cttcagatgt ccgctggctg ttaagcgctt
     3361 catcattacg cgacaaaatc accaggcaac gatcgcgatt cttctcgcgg aagttggtca
     3421 cacacttagt ggcaatatcg gcatactctt ccgggcgatc aatcttgcct tccatgttct
     3481 cataagggaa caaattaggg ttgaagatca cctggcggat atcgcaaaga aaaccaatcc
     3541 gttccgccca gtatccgccc agaccaacgc cgcaaatcag gggacgctcg tcgacgttca
     3601 gttgcaacat tttgtccact tctttgagca gatgctgcat atcatgtttc ggatgccgcg
     3661 tactgtagct tatcaagcgt acatccgggt caataaactg caattgtaag actttctcgt
     3721 ggttacccgg actgttagag tcaaaaccgt gtaaatagat aatcatcgct tcctcacata
     3781 agccagggtt agtgacctgc tttctcttcc tgccagcgtt catgtaactg attcagacgg
     3841 gcgctgatcg ctttccagcg agccgagtcc atcagttcct gtcgcgaaaa tgaaccttta
     3901 tgatacaaac gtgtaacacg ttctgccttg atcggtgaca gattatctaa cacgctgacg
     3961 gcccctttac gattattgca gaccaggatc atatcgcaac ccgcatccag tgatacctgc
     4021 ccgcgttcgg cataactgcc cataatcgcg gcaccttcca tcgataaatc gtcagagaaa
     4081 atcacgccgt caaaacccag ttcctgacgc aaaacggttt tcagccagta ggaagaaccg
     4141 ctcgccggac gcggatcaac atcactgtag atcacatgcg caggcataat ggcgtcgagt
     4201 ttattttcgc ggattaacgt gctgaaaacc gacatatctt tggcacgaat ttccgcttgc
     4261 gggcgtgggt cgcacggcgt ctctttgtgt gaatctgcag ttactgcacc gtgtcctggg
     4321 aagtgtttcc cggtcgtttt cattccggct tcatgcatcc catcaataaa ccgactggcg
     4381 attgccaggg ctttttctgg gtcggcatga taagaacgct cgccaatcgc cgcgctgata
     4441 tgtcctacat ccagcactgg cgcaaagctg atatcaatat ccatagcgat catttcgctg
     4501 gccatcagcc aacccgcctc ttgcgccagt ttgccgccct cttccattcc caacagcgca
     4561 gcaaaggatt gtgctgccgg taagcgggta aaaccttcgc gaaaacgctg gacgcgtcca
     4621 ccttcctgat ctaccgccac caccagatga ttgcgcgatg ctgcgcggat ctggcgcacc
     4681 agttcacgca actgggcagg atcatgatag tttcgcgtaa agagaatcag ccctcccacc
     4741 agcggatgcg ccagtatttc acgctcttcc gcgtccagtt cgtaaccttc gacatccaac
     4801 attactggac ccacactgct ctccttattg ttttattaat agctgccgcc aggtgtcatc
     4861 ggccagcctg ataaattgtt gatcgccggt ttgtcgccag cggtactcaa accaccctgc
     4921 tttgagcatc agcagccagg gaaaccatcg cctgacctga cgccataatt gcgccggata
     4981 aatcttcgcg cgagtggcat agtcattgac caattgccgg tgctgttcag tattttccac
     5041 ccacaccgcc gccagttcca gcgcgatatc accatctcct gcatactccc agtcgatgag
     5101 ttttaaccct gacgcgctat gcactaaatt tccggcgtgg acatccatat gcaatggact
     5161 taagcgtaaa ggccgtggtt cccgcgcttt gcgcagacgt tttaacattc gcagccaacc
     5221 cactgtccgc cgcgccggat cgctttgctg ccagtacagt tccagtaacg gcaacagcgt
     5281 tattcgccag ccaaaacgtg gttgttgatg tagataatac agcaagcctg ccagttcgtt
     5341 ggtatccggc aaatacgttt ttacctcgcc gggcagatag tcgactacca tccagtcacg
     5401 gagatataaa tgcggcttcg gtgcaatgca tgcgggtagt tgtgataaag cccgatactg
     5461 gcgcaagaac gcggactgcg gcgcatcagg atcgtgcggc tgacgcacaa caaaacgctg
     5521 gccctgatgt tcaatgagaa aactaccgcc actaagccca ctattaaacg tcgtgacggg
     5581 atgaaactgc gggaaaaagc gcgacagcaa ttcgtcgcgc gtgatgggat tattgctgcg
     5641 aaacggcacc tttacctgac cagataattt cgcccgtctg caccagcatc agctgcattt
     5701 gtagggtcgg agcgttaacg ttgccagagg cgctggagta cagcacgtaa tgagcgccga
     5761 cattgcgggc aatgcctatg gctttactac gggtgcctaa actatcctgc ggcgacaaac
     5821 ctaactgttg cttagccatc gacagctgct gggcggaaac cagggtaaat ttcccgttat
     5881 tagccagcgc atttcgcagc gtttcggtcg cttctgcggc attcagcgaa ccgttagtac
     5941 ggttgttaac gctatcaacc agcaggacgc tacccgcagt caccccgtca gccccaagca
     6001 tcttactgac catcggctgc attgcgccat tccagtcata atggcgaata tgcggcgcag
     6061 gcggtgcagt ttgatcttcg tgctcaattg ggcctggctg ctgcgggatc gtcggcaccg
     6121 agggcactgt ggggacaggc tgttgtggct cggctggttg ttccggcgct ggtttcactt
     6181 cttctaccgg tgcaggttca cgttgcccca cacacccggc gagaaacatc gccagcgcgg
     6241 taatcaaggc gtagcgactc atttttgtca tcaagattca ccccttacaa atatagataa
     6301 agtctgactt tgtgcgcccc cagaaaattg gcgctgccgt acagcgttac cgccgaatgt
     6361 gcgggaatgg taacgctgcg tggcctttcc agaggatgca tctccagccc tctggcgtca
     6421 taccagtaaa aacgataatg aacggtaacg ggttcttgcc tttcgttata aagcgttgag
     6481 gatgctgaag gttgaatatc agacgtcgaa aggacgggct tttctgcact gatgcccgca
     6541 gccaataaag atgactccat caccagcgat tgctcatcat tcaccggaat ttccggatgt
     6601 gaacgacagc ccaccagcag taacaacgcc agagacacca gcccaaagca tccttttctc
     6661 atcgttacag acctttatgc gccagcattg gtcccagcgg acggccaccc aacaagtgca
     6721 tatggatgtg gtaaacctct tgtccgccat ggcggttggt gttcatgatc agacgatagc
     6781 catcttcggc aataccttct tgctcagcaa tttttgccgc tacggtgatc atgcgtccca
     6841 gcgcctgctc atgctcagct gagacgtcgt tcacagtcgg aatgaggata ttcggaatga
     6901 tcagaatatg cgttggcgct tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct
     6961 ggtagacgat atcggagggg atctcacgac gaataatttt gctgaatata gtttcttctg
     7021 ccacgacatt ttcctttttc aaatagccat gcgttgcgcc atgctacgcc gcgccacact
     7081 gcgagtatag agtatggtcg agttaaacct gctctttcaa ccttaactca cgatttctta
     7141 agcaaaaaat cgttttgctg ccgtatatat ctccattatt cccatttctg cctgcgaatg
     7201 cgctgaaaag acattctctg tttggtttac acgacaaata tgaatgcgta tatttctcat
     7261 ttgcatttgc atttgcaggc aaaattattc gcacactaaa tataaccgct catcaccctc
     7321 ctccggcatg agcctgacga caacataaac caagagattt caaatgcttt caacacaatt
     7381 taacagggat aatcaatatc aagccatcac caaaccgtca ctacttgccg gttgcatagc
     7441 actggcacta ttaccttctg ccgcttttgc tgcaccagcc actgaagaaa cggtgattgt
     7501 tgagggttca gccacagctc cagatgatgg cgaaaatgat tacagcgtaa cgtctacctc
     7561 tgcgggtacc aaaatgcaga tgactcaacg tgatattcct cagtcggtca ctattgttag
     7621 ccagcagcgg atggaagatc agcagttaca aacgctgggc gaagtgatgg aaaacacgct
     7681 ggggatcagc aaaagtcagg cggattccga tcgtgctctt tattattccc gcggattcca
     7741 gatcgataac tatatggttg atggtatccc cacctatttt gaatcgcgct ggaatctggg
     7801 cgacgcactt tctgatatgg cactgtttga acgcgtagaa gtagtgcgtg gcgcgacagg
     7861 actcatgacc gggacgggta atccatctgc ggcaattaat atggttcgaa aacacgcgac
     7921 cagtcgtgaa tttaaaggcg atgtctcggc ggaatacggt agctggaaca aagaacggta
     7981 tgtggcggat ttacaaagcc cactcaccga agacggtaaa atccgcgcgc gaattgtcgg
     8041 cggctaccag aataacgact catggctgga ccgctacaat agtgaaaaga ccttcttctc
     8101 cggcattgtc gatgctgatt taggcgatct tacgacgctt tcagccggtt acgaatatca
     8161 gcgcattgat gttaatagcc caacctgggg cggtttaccg cgctggaata ctgatggcag
     8221 cagcaacagt tacgatcgcg cacgcagtac cgcacctgac tgggcgtaca acgataaaga
     8281 gatcaacaag gtctttatga ccctgaagca gcggtttgct gatacctggc aagcgacact
     8341 gaatgccacc cactctgaag tcgaatttga cagcaaaatg atgtatgtcg atgcctatgt
     8401 aaacaaagcg gatggtatgc tggttgggcc atacagtaat tatggacctg gctttgatta
     8461 tgtcggcggc accggttgga acagtggcaa acgtaaagtt gatgcgctgg atttgttcgc
     8521 tgacggtagt tatgaattgt ttggtcgtca gcacaatcta atgtttggtg gcagttacag
     8581 caaacaaaac aatcgttact tcagttcatg ggccaacatc ttcccggatg aaattggcag
     8641 tttctacaac tttaatggca atttcccaca aaccgactgg tcaccacaga gcctggcgca
     8701 ggacgatacc acacatatga aatcgttata tgctgccact cgtgtcaccc ttgccgatcc
     8761 gctgcatctg atcctcggcg cacgttatac caactggcgg gttgatacgc tgacttacag
     8821 catggagaaa aaccacacca cgccttacgc tggtctggtg tttgacatca atgacaactg
     8881 gtcgacctac gccagctata cctctatttt ccagccgcaa aatgatcgtg acagttcagg
     8941 caaatatctg gctccaatca ccggtaacaa ctacgagctg ggtctgaaat cggactggat
     9001 gaatagccgt ctgaccacca cgttagccat cttccgtatt gagcaggata atgtcgctca
     9061 gtccaccggt acacctatcc ccggcagcaa cggcgaaacc gcctataaag cggtggatgg
     9121 gacagtcagt aaaggggtgg aatttgaact caacggcgca attaccgaca actggcagct
     9181 gacatttggc gcaacgcgct atattgcaga ggataacgaa ggaaacgccg ttaatcctaa
     9241 tctgccacgc accacggtta aaatgttcac cagctatcgg ttgcctgtca tgccagagtt
     9301 gacagtcggc ggtggtgtta actggcaaaa tcgcgtgtat accgacaccg tgacaccgta
     9361 tggcaccttc cgcgccgagc aaggtagcta cgcgttggtg gatctcttca cccgctacca
     9421 ggtgacgaaa aacttctcgt tacaggggaa cgtcaataac ctgttcgaca aaacctacga
     9481 taccaacgtg gaaggttcta tcgtctacgg cgcaccgcgt aatttcagca ttaccggcac
     9541 gtatcaattc tgaataacac ctgtaaaaaa ggcagccatc tggctgcctt agtctcccca
     9601 acgtcttacg gattagtggt tacggatgta ctcatccatc tcggttttca ggttatcgga
     9661 tttagtaccg aaaatcgcct gaacaccaga accagcaacc actacgcccg ctgcgcccag
     9721 tttcttcagg ccagcctgat ccactttaga cacatcagca acgctgacgc gcagacgggt
     9781 aatacatgcg tcgaggttag taatgttttc tttaccacca aatgcagcaa ccagagccgg
     9841 tgccatttcg ctggtacctg tcgcttttgc atcttcagtc gcgtcttcac gacccggcgt
     9901 tttcagatcc agtgctttaa tcagcacgcg gaagatggtg tagtaaacaa tcgcataacc
     9961 gataccgacg atcgggaaca gccacagttt gctgctgtta ccagacagaa cgatgaagtc
    10021 gatcagaccg tgcgaaaacg acgtaccgtc acgcatcccc agaagaatac agattgggaa
    10081 tgccaggcct gccagaatcg cgtggatgat gtacaggatc ggcgcaacga acatgaagga
    10141 gaactcgatc ggctcggtga taccggtcag gaacgaggtc agcgccgcgg agatcataat
    10201 accgcccact ttcgcgcggt tttctggttt agcagagtgc cagatagcaa ttgcggcagc
    10261 tggcagaccg tacattttga acaggaagcc accagacagt ttacccgcag tcgggtcacc
    10321 cgccatataa cgcggaatgt cgccgtggaa aacctgacct gctgcgttgg tgtattcacc
    10381 aatctgcatc tggaaaggta cgttccagat gtggtgcaga ccaaacggta ccaggcaacg
    10441 ttcgatgaaa ccgtaaatgc caaacgcaac taccgggttc tggtaagcag cccactgaga
    10501 gaaggtctgg attgcagaac caatcggcgg ccaaatgaag gacagcacaa cgccagtaaa
    10561 gatggcagcc aggccagaaa tgatcggcac aaagcgttta ccggcaaaga agccaagata
    10621 ctcaggcagc ttaatacggt agaaacggtt aaacatgtac gctgcgatcg caccggagat
    10681 aatccctccg agtacgccag tatccgccag gtgtttagag gcgatttctt cagcaggtaa
    10741 atgcagtacc agtggcgcaa ccacggccat ggttttaacc atgatgccat aggcaacaac
    10801 tgcagccagc gcggatacgc catcgttatt ggtaaagccg agggcgacac cgatcgcaaa
    10861 aatcagtggc atgtttgcaa agacggaacc gcctgcttct gccataacat gcgatacaac
    10921 ggcgggcagc cagctgaaat tcgcggaacc gacgcccagc agaatacctg cgataggcag
    10981 tacggatacc ggcagcatca gcgatttacc gaccttttgc aggttagcaa atgcattctt
    11041 aaacataatt gagagtgctc ctgagtatgg gtgctttttt tacgttctca cgcgtggcaa
    11101 ggggggagag cctcgccgtg tacagggcat ctaagcgccc tttatttatt acacagagta
    11161 aaataattca gtgccaattt gtttgacggc tatcacattt caacttcaga tcattggaga
    11221 agttgcacat tttgacaaaa catatgtcac aatatttcaa acgtggttat tcaccgccca
    11281 attttcggcg gtgaacttta ctcgttttag tcattaatta cgagctttaa aaaaactcat
    11341 tcaacggatg gattgaaggc gggaagcgtc gatgtgaaac agacgggcga agttatcggt
    11401 ggttacctgc gccagttctt caacggcaac acctttcaac acagccatgt attctgcaac
    11461 gtcacgaacc atcgcaggtt gattctcttt tcctcgatgc ggcaccggcg caaggtaagg
    11521 tgagtcagtt tccaccagta accgatccag ggggacataa cgcgcagcat cgcgcagttg
    11581 ctccgcatta cggaaggtca caatgccgga aaaggagatg taaaatccga gatccagtaa
    11641 tttgcccgcc gtttctctgt cctctgtaaa acagtgtagt acgccaccgc aatccgtcac
    11701 tttttcttcg cgcagaattg ccagcgtatc ggcgcgggcg tcacgggtat ggacgataac
    11761 cggcttgttc agttcacgac caatctggat atgatggatg aaggactctt gctgacgtac
    11821 tttagtttcc ggtgtgtaat aataatccag cccggtttca cccagcgcta caacaccctc
    11881 ttctgccgcc agacggcgta aatcttctac atcgtaggga tcattctggt tgagtggatg
    11941 cacgccacaa gaaaatacga cgttgtcacg ttcgcctacc agatcccgca tgtgtaaata
    12001 acccggtaat gttgtggcga ctgccagaca aaatttcaca tcgcgtgcgg cggctttcgc
    12061 cagaacgtca tccacgtcct tatgcaaaga ttcataatcc agaccatcga gatggcagtg
    12121 tgagtcgact aaaaacatga tgtctctctt aaagatgagg aaccggtagc acaacgcccg
    12181 gttgcaggta atgctcaata cgcagtaaaa gatcggtgat gagaagctcg cggttgatgc
    12241 ctgtgacaga cattaactgt tcacgaatgt ggcaaacatc ccccagtata gcctgcaggc
    12301 gcgagggaga aagatggttt gccagttcgg cgaccaggcc cggcacatca acattggtca
    12361 cctgcgcagc gccatgatgg cgttttagcg catccatcag caacgttgcc agccagtgta
    12421 aacgcgccgg agcttgttca tgattaaggg ccgccagcag cgaataccag tcgcccgatg
    12481 gcacgctata tgccaacgcc tgacacaatg tttcacgagc ctgccagtta tctccctgaa
    12541 acaacgccag tgccgcgcca ggcgaaccgg cgcttaagcg caatgcggca agtaatgcat
    12601 cctgtgacat tgtcacttcg cgtgaaagcc aggtcacggc gtactgttcc ggcggcggcg
    12661 caaggtaatg taaccgacaa cgactacgta acgttgccag taaacgttca ggctcgcggg
    12721 tagccaggaa aaaccaggtt tctgctggtg gctcttcaag cgttttcagc aatgcgttag
    12781 ccgcggcgtc ggtcagtaag gcagcatcgg ttacccagac aactttcgca ccacctaagc
    12841 gtgcgtgctc attcagcttt tcggtgacct cacgtaccgc atcaatgccc agcgtatttt
    12901 tccctttttc gggagccagg gtgtagtaat cgggatgcgt gccagcctgc atcaactgac
    12961 atccacgaca gtgaccgcaa cttttgtggc cctgcggttg ttggcagagt aaataacggc
    13021 tcagggcgta gattaaagca tcatcgccca tgcccggtaa cgcctgaatg agtagcgcat
    13081 ggtgacctct tccggcctga tagctggcta ccagtttttc gaaatcaggt cgtaaccatg
    13141 gataccatct catgcgtcca actccttcac ccagcgggtc acggtagtgc ggattgcatc
    13201 catcacggcc tccagcggct gggtggcatc aatggtatga atacttttat cttgtgcagc
    13261 cagttccaga tagcgggcgc gggtgcgatt aaagaaatcg aaagactctt gctcaatacg
    13321 atccagctcg ccgcgcgcac gcgcgcgttt taagccaact tccggggtaa catcgagata
    13381 gagcgttaag tcggggcgaa aatccccgag aacagcatca cgcagtgttg ccagcatatg
    13441 ttggtcaata ccacgtcctc cgccctgata cgcctgagtg gagagatcgt ggcgatcgcc
    13501 aataacccag gtgccgttag ccagcgcagg tttgatgacc gtttctacca gttgaacgcg
    13561 cgcggcataa aacatcagaa cttcggcttt atcggtaatg atttcatcgc ctaccgattt
    13621 gatatccagc accaggcttc ttaacttttc ggcaagttgc gtaccaccag gttcccgagt
    13681 gaaaaccatg tcgcggatac ccagttgctc gagcgtctca accaccacat tacgcgcggt
    13741 agttttgcct gcgccttcca gcccctcaat gacgatatac ttactgcgca tttttttcct
    13801 taagcacttt cagataatcc tgcacagact tgttatgact ggcaagattg gtattaaacg
    13861 tgtgaccacc tttaccatcg gccacaaaat agagatacgg cgtttttgcc ggatgcgcag
    13921 cagccttcag cgaatccgcc cccggcgtag ctatcgcacc cggcggcaaa ccggtaatgg
    13981 tataggtgtt atacgctgtc ggcgtttcca ggtctgcacg agaaagtttg ccattataac
    14041 gctctcccat cccgtaaatc acggttgggt cggtctgcag gcgcatacca atgcgtaaac
    14101 ggttgataaa taccgaggca acctgatcgc gttcactggc aatggcggtt tctttttcga
    14161 taattgatgc catcgtcacc agctgatttt tatctttata aggcagaccg tccgcacgcc
    14221 cttcccaggc gctatcgacc gctttcacca ttttcttgtg cgctcgcttg agtaacgcga
    14281 catcggtggt attggcggta tacatccagg tgtctggcca gaaccaacct tcaatccact
    14341 ccgggttttc cagttcaagt gcctgcgcta cggtgatgta cttatcatcg ctcagcgtgt
    14401 gcttgatata cggggcctca cgcaattgct tgaggtagtc gctcagacgc atcccttcta
    14461 ccagtcgcag agggaactgt gcttctttac cgctttccaa caatttcagc atctcgcgca
    14521 cggtcatctg cggtgtaaag cggtaagtcc cggctttaaa gtgagaaaga tccggttcga
    14581 tacgcagcaa ccattgaaaa acccgtgggc gattgatgat cttatcggca taaagctgtt
    14641 caccaagcgc cagacgtccg gtccctggct tcagggtaaa tatcgtctct tctttgataa
    14701 gcaatttgct gtcggcaaga tagcgaacct tccagacgcc cacaccagcg gcgataccca
    14761 gtaccaccag caataacaag attatcaata acactttttt catgactaat tcgggcgctc
    14821 acaaagtggg gctaaatatt catataacgt tgctgacgaa aaggagacat cgccacaggc
    14881 acgtacgggc atcactggca ttaacgcatt acaaataacc atctcatctg cctgcaacgc
    14941 ctcttccaga gaggcttgca cttcgacaag ctgataagag gattgtgcca gcaaacggat
    15001 acagaattgt cgcataatgc cgttaacacc tgcctgatcc agtcgcggcg tataaactac
    15061 gttgcccttc cgccagaaca aattagctgc acagcattcc gtaacccccc cttcgctgtc
    15121 aaggaccagc gcctcatcag cgtttgtctg ctcaagatga gagcggatca atacttgctc
    15181 aagccgatta agatgtttaa tacctgcaag atgaggattg cgccccagcc gcaccgggct
    15241 tagcgccaac gtgatccctt cgttacgcaa acggtcgtaa tgtgcaggat aagccgtaac
    15301 ggagagaatc cgcgttgccg gtccactgtt caatgtgctg taccctcgcc cgccactacc
    15361 gcgactgatc acgactttca gtacaccatt ttgctgttct gctgccagcg ttttcatctc
    15421 ttgttcaagc tgaggccaga agtcacagga aatcatcaac cgctgacaag catcctgtag
    15481 tcgctggata tgcgccgata acaaactgac tttaccgtcg ataactctgg cggtggtaaa
    15541 acaaccatca ccaaactgcg ttgcccgatc gcttactgac agcgattcct gcttataacc
    15601 gttaattaag aacataccgg ctccttatca gagaattgtt agtgtggcag catgttcact
    15661 acggaacaag tcggaataaa agctaagaaa aaaggcccgc aagcggacct tttatatggg
    15721 tggaaaatga caacttagat ctttttaaag atcaaagaac cattagtgcc accgaagccg
    15781 aaggagttac acagcgtgta ttccattccg ctgacctgac gtgcttcatg cggtacgaaa
    15841 tccagatcgc aaccttcatc cgggttatcc aggttgatgg ttggcggaac agcctgatcg
    15901 cgcagcgcca ggatggagta gatagattct actgcacccg ccgcacctaa caggtgaccg
    15961 gtcatagatt tcgtggagct taccaacaca cggcttgcag cttcaccgaa gatggttttc
    16021 accgcctgcg cttcagcttt atcgccagcc ggcgtagaag taccgtgcgc gttaacgtag
    16081 ccaatctgac tcgcttcaat gcctgcatca cgcagagcat ttgccattgc cagagctgcg
    16141 cctgcgccat tttctggcgg tgacgtcata tgataagcat cgctgctcat accaaagccg
    16201 acgagttcag cgtaaatttt cgcaccgcgt tttttcgcgt gttcgtactc ttcaagtacc
    16261 agcataccgg caccatcgcc cagtacgaaa ccatcacgct ctttatccca cgggcggctc
    16321 gccgcttgcg ggttatcatt gcgggtagat aatgcacgtg ccgcgccaaa accaccaaca
    16381 cccagcggcg tactggcttt ctctgcgcca cctgcaacca tcacgtcagc atcgccatac
    16441 gcgataatac gcgcagcatg gccaatgttg tgcacgccgg aagtacaggc ggtcgcgata
    16501 gagatgcttg ggccacgcag gccatacatg atagtcagat gacctgccac catgttcaca
    16561 atcgttgacg gaacgaagaa tgggctgatc ttacgtggac caccgttcat cagagatgtg
    16621 tggttttctt cgatcagtcc gaggccgcca atcccggagc caattgcggc accaatgcgg
    16681 gttgcgttct cttccgttat ttcaaggcca gaatcctgca tggcctgaac gccagcgaca
    16741 attccatatt gaatgaaggc atccatcttg cgctgttctt tgcgcgagat aatgtcctca
    16801 cagttaaaat cctttactaa gccagcaaat ttcgttgcat aggcgctagt atcgaaatgg
    16861 tcgattaggc tgatgccact ctgaccggca agcagagctt tccaggtaga ctctacggta
    16921 ttgccgacag gagacaacat gcccagtccg gtcacaacta cacgacgctt agacacgttt
    16981 gtcctccagg gagggaaaaa atgattctag tgggacaaaa agataaaact caggcggtcg
    17041 aacgaccgcc tggagatgtt cacttacgcc tggtggccgt tgatgtaatc aatggcagcc
    17101 tgaacggtgg tgattttctc agcttcttcg tccggaatct cagtatcaaa ctcttcttcc
    17161 agagccatta ccagctcaac ggtgtcaaga gaatccgcgc ctaggtcttc aacgaaagaa
    17221 gcattgttgg taacttcttc ctgcttaacg cccagctgtt cgccgataat tttcttaacg
    17281 cgttcttcga tagtgctcat actcttaaat ttcctatcaa aactcgcttt cgcgatggtt
    17341 ttcgtagtgt ataaaatgtt gaaaaatttg caactaaatc ccggcaggtc ttaccacgat
    17401 tttacgttat tttgaggcct taccccccaa taacgcaaat cattttgcac taattattac
    17461 tcataaccac gcagactgcg cgcaacatga gcattttgtg caaatcgcgg tcagaccatg
    17521 tacatcccgc cgttcacatg caaagtttca cccgtgatgt aagctgcttc gtcggatgcc
    17581 aggaatgcaa ccgcgttggc gatttcctgt gcgccgccga ggcgacccgc aggaacctgc
    17641 accaggatac ccgcacgctg gtcatcgctc agcgcacgtg tcatgtccgt ttcaataaag
    17701 cccggagcaa caacgtttac agtaataccg cgtgacgcaa cttcgcgcgc cagtgattta
    17761 ctgaagccga tcaagcccgc tttcgccgca gcgtagttgg cctgaccgcc atttcccatg
    17821 gtaccaacca cagaaccgat agtgataata cgaccatgac gctttttcat catagcgcgc
    17881 attaccgctt ttgacagacg gaaaacagat gaaaggttgg tttcgataat atcgttccac
    17941 tcttcatctt tcattcgcat taacaggtta tcacgagtga taccggcatt attgaccagg
    18001 atatccactt caccaaattc tgcgcgaatt ttttccagaa cagattcgat agatgccggg
    18061 tcggtcacat tcaacatcag acctttgccg ttagcaccta aataatcact gatcgcctga
    18121 gcgccatttt cactggtcgc agtgccaata actttcgcgc cacgggctgc gagcgtttca
    18181 gcaattgcgc ggccaattcc gcggcttgca ccggttacca gtgcgatttt tccttcaaaa
    18241 ttcatgatgt tcctctttta aagctcgagc gccgctgcca tcgctgaagg ttcgttcagc
    18301 gccgaggcgg tcagggtgtc gacaatgcgt ttcgtcaggc cagtaagcac tttgcccggg
    18361 ccgacttcat agagatgttc tacgccttgc gctgccatgt actcaacaga cttcgtccac
    18421 tgaaccgggt tatacaactg acgtaccagt gcgtcacgaa tggcatcacc attggtttcg
    18481 catttcacat caacgttatt cacaacagga actgttggtg cgttaaaggt gattttcgct
    18541 aattctactg ccagtttgtc ggctgctggt ttcatcagcg cacagtgaga cggtacgctc
    18601 actggtaacg gcagagcgcg ttttgcgccc gcagctttac aggcagcgcc agcacgctca
    18661 accgcttctt tatgaccggc aataaccacc tgtcccggag agttaaagtt taccggagaa
    18721 acgacctgac cttctgcagc ttcttcacac gcttttccaa tagacgcatc atccagaccg
    18781 atgattgccg ccatagcgcc cgttccttcc ggtacggctt cttgcatgaa cttgccgcgc
    18841 atctcaacca gacgcaccgc atcagcgaaa tcaatcacac cagcgcaaac cagcgcggag
    18901 tattccccca ggctgtgacc ggccatcatt gccggtgctt taccgccatg ctgctgccat
    18961 acgcggtaca gcgcaacaga ggcagtcaac agcgctggct gggtttgcca ggttttattc
    19021 agttcttcag ctggcccctg ctgggtcagc gcccacaggt cgtagcccag cgccgcagaa
    19081 gcttcagcaa acgtttcttc gacaattgga tagctcgccg ccatatcagc cagcattcca
    19141 acggtttgag aaccctgtcc agggaacaca aatgcaaatt gcgtcatgtt ttaatcctta
    19201 tcctagaaac gaaccagcgc ggagccccag gtgaatccac cgccaaaggc ttcaagcaga
    19261 accaactgcc ccggcttgat gcgcccgtcg cgtacagctt catccagcgc gcacgggaca
    19321 gaggccgcag aggtattacc gtggcgatcc agcgtcacca cgacattgtc catagacata
    19381 ccgagttttt tcgccgttgc actgataata cgcaggttag cctgatgcgg aaccagccag
    19441 tccagttgag aacggtcaag attattcgcc gccagcgtct catcaacgat gtgcgccagt
    19501 tccgttaccg caaccttgaa gacttcgttg cccgccatcg tcagatgaat tgaattctct
    19561 ggattcacgc ggtcggcgtt tggcagcgtc agcaattcac cataactacc gtcggcatgc
    19621 agatgggtgg aaatgattcc cggctcttca gaggcagcca gcaccgcagc gcccgcgcca
    19681 tcgccaaaaa taataatagt cccacgatct gttggatcgc aggtgcgcgc cagtacatcg
    19741 gaaccgacga ccagagcata cttcaccgcc ccagatttca cgtattgatc ggctacgctt
    19801 aatgcatagg tgaaacctgc gcaggctgct gcaacgtcaa atgccgggca acctttaatg
    19861 cccaacatgc tttgaatctg acaagctgcg ctcgggaaag cgtgcgtagc agaagtcgtt
    19921 gccacaacga tcaggccaat ctggtctttc tcaatgcccg ccatctcaat tgcgcgtgtc
    19981 gccgcttcaa agcccatggt tgaaacggtt tcgtttggcg cggcaatgtg gcgttcgcgg
    20041 ataccggtac gagtgacaat ccactcgtca gaggtgtcca ccattttttc caaatcggcg
    20101 tttgtccgca cttgttcggg cagatagctg ccagtaccaa taatcttcgt atacatgtac
    20161 gctcagtcac ttttcggtta tataccgtca cttgcaaact gcgagttcgc tggcagcgtc
    20221 ctgctaccgc agagttccgc ttttgccacc gtccagcagc tcaaaaccag ctgggtatac
    20281 agattccagg cgagcggcaa ttcgctgagg aacttgtcgc tgcaccgcct gcactgcctg
    20341 ttcaatcgcg accgcaaaag ctcgctgatt ggctgcacca tgacttttta tcaccgtgcc
    20401 gcgcaatcct aacagacagg cgccgttata ctggtcgggg ttgaggtgac tgaatcgcct
    20461 cgtcaggctc ttttgtagcc aacgctttaa taacagtagc caccacgacc gttttttccc
    20521 ttcaccctga gatttcagca gagaaaggaa catcctgaca acaccttcca tcgtctttaa
    20581 tgtgacattt cctgtaaagc cgtcacaaac cagcacatct gtcttgccag ttaacaactc
    20641 attggcttca agatagccga tataattgat agaagggatt gttttaagca ccgctgaggc
    20701 atcccgaata ctatcgagac cctttacttc ttcttcacca atattgagca acgccacgcg
    20761 aggattggga atttccacca cctcttcagc caggactgag cccataatgg caaattgcac
    20821 caacattgtg ctgtcacaat cgacgttggc ccctaagtca aggaccaccg ttttgccctt
    20881 ttgctgatgt ggtaataccg tcaccagcgc cggacgctca atcccctcca ggggcttgag
    20941 taataatttt gccagcccca tcagtgcccc ggtattaccg gcactgacac aggcttgcgc
    21001 tcgaccttct ttcaccagct ccagggccac gcgcattgaa ctcccacgac tggcgcggat
    21061 agcttgcgaa ggccgggcat cactggcgat aactgactgc gcaggaataa tctgcagacg
    21121 cgaacgttgt tcaaagtcag ctttagcaag taatggcgtg atggcgtcgg gattgccgac
    21181 taaaagaaga gtgagttgcg aattagaatt cagtgcctgc aatgctgcag gcactgtcac
    21241 ggaagggcca aaatcccctc ccatgacatc taacgccagg gttagacgtg tcaaggtatc
    21301 gtcgccgccc ggtttggtct ttccccagtt gcctggggaa aatcctcact aagcttcatc
    21361 acgcagacgc gtgattactt agcgatgacc ttgcggccgc ggtagtaacc gtcggcagtg
    21421 atgtggtgac gcaggtgttt ttcaccagaa gttttgtcta cagacaggct ggtgactgcg
    21481 gtcagcgcgt catgggaacg acgcatgcca cgtttggaac gggttggttt attctgttgt
    21541 acggccatgg accttactcc tcaattacgg tttatccccg atccaacggg gagcaccaat
    21601 tacttacgct ttaagctggc taatacggca aatgggtttg gcttttgcgc ttcttcaggc
    21661 agttcaccaa agaccatgtc cgcttcggac acttcacagt gttcagaatc atgcaccgga
    21721 actaccggca aggcgaggat gatttcatct tcaaccattg caagcagatc gatttcaccg
    21781 aattcgttaa cctcaatcgg ttcatacgct tccggcagtg cttcagcctg ttcgtctgaa
    21841 cgcacaggac taaaacaata cgttgtgtag acctgatgag taaacggctt cccgcaacgc
    21901 tgacactcga gcgttaccgt caccttcgca tcgccgttta acactgcgag acgttggtta
    21961 tcgatagcga acgacatgga gcattccaca tcactgtcca cactgactac ggattcggcg
    22021 acgcgctcaa cctgatcagg agtatagata ccctggtaat caaggcgttt ttgagccgta
    22081 cgaaccggat cgagagtcag gggtaatttt accttttgca tagggcgcgc atattaactt
    22141 tgtaacgtca tagagtcaaa gaaaaaggca gcctgtggtt gccttttgcc aataattcgc
    22201 acacattgcg ggttactgct ttattttcgt tcagtgctga taacgaacat caacctgcaa
    22261 aacgaagcgc atagtttaaa atggctatca tcaatacgct atatctgatg gaaaaaaata
    22321 tgcctaaact tattttagcc tccacctcgc cctggcgtcg cgctcttctg gaaaaactgc
    22381 aaatctcttt tgaatgtgca gcacccgagg tcgacgaaac tccacacagc gacgaatcac
    22441 cgcgacagtt ggtgcttcga ctggcacaag aaaaagcgca atctctggcg tcacgttatc
    22501 cggatcattt aattattggt tcagaccagg tatgtgttct tgatggcgaa atcaccggta
    22561 aaccgttaac ggaagaaaat gcccgtctgc aattacgcaa agccagcggc aatatcgtca
    22621 ctttctatac tggactggcg ttgtttaatt cggcgaatgg gcatctgcaa acagaagtgg
    22681 agccttttga cgtccatttc cgtcacctga gcgaggcgga gattgataat tacgtgcgta
    22741 aagagcatcc cctgcactgc gcgggtagct ttaagagtga aggatttggc attactctgt
    22801 ttgagcgttt agaggggcgt gatcctaaca cgctggttgg tttaccgcta atcgcgctgt
    22861 gtcagatgtt acgtcgggaa gggaaaaacc cgctgatggg atagtttttt gcgccggatg
    22921 cggttacgtc gcatccggca atcggtgctg gcggcctgat gcggcgtaaa cgccttatcc
    22981 cattatcatg cttatatgct cgtattagcg cgcgttacgc agcttttgca aacaacgctt
    23041 caaaccatca tccatcggcg cttcgatacg catcacctca ccggtccccg gatgggtaaa
    23101 cttcaacgct gcggcgtgca ggaacagacg atttaatccc gtgcctgctt cagtgagctg
    23161 tctgtcaaat tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ctgcatactg
    23221 tgtatgcaca cggatctgat gagtgcgccc tgttaccgga ctacaacgca ccagggtggc
    23281 aaatgcatag cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactcac
    23341 acgcacgata cgttcgccgc tttgcagaat atttttcaat aacggcgcct gaacgctctt
    23401 cacatgcgac tgccactgac cgcgcaccag cgccaggtaa tctttttgca tccctttttc
    23461 acgtaattgc tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc
    23521 tgaggtgtcc cggtcaagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc
    23581 ccgcaaacct tcaataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga
    23641 aggtttattc agcaccagga tgtgatcatc ttcatataag atgacgtccg ccagcgccgc
    23701 caccttttgc agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg
    23761 aatacgcacc tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac
    23821 ccgcacttcg cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg
    23881 ggtacgcaaa aagttgtcga tacgttgccc cgcttcgtcg gcggtgatag caacaatttt
    23941 tacggatgga gtctctgttt tcatggtggg cgattctaat tagccaacag gattcgcgcc
    24001 actcattttt ctatgcttat atttagtttt gcaccttatt acttcactac gtgatcactt
    24061 tattgatggt tattaaacca atcaccagca agaagtgaaa aaactgtgag taagcgggtg
    24121 ataaatggta aaagtcatct tgctataaca aggcttgcag tggaataatg aggccgtttc
    24181 cgtgtccatc cttgttaaaa caagaaattt tacggaataa cccattttgc ccgaccgatc
    24241 atccacgcag caatggcgta agacgtattg atctttcagg cagttagcgg gctgcgggtt
    24301 gcagtcctta ccggtagatg gaaatatttc tggagagtaa tacccagtct gtttctttga
    24361 taattgcgct gtttttccgc atgaaaaacg ggcaaccgac actctgcgcc tctttgagct
    24421 gacgataacc gtgaggttgg cgacgcgact agacacgagg ccatcggttc acacccggaa
    24481 aggcgttact ttgcccgcag cttagtcgtc aatgtaagaa taatgagtaa gttacgatga
    24541 aaagaatgtt aatcaacgca actcagcagg aagagttgcg cgttgccctt gtagatgggc
    24601 agcgtctgta tgacctggat atcgaaagtc cagggcacga gcagaaaaag gcaaacatct
    24661 acaaaggtaa aatcacccgc attgaaccga gtctggaagc tgcttttgtt gattacggcg
    24721 ctgaacgtca cggtttcctc ccactaaaag aaattgcccg cgaatatttc cctgctaact
    24781 acagtgctca tggtcgtccc aacattaaag atgtgttgcg tgaaggtcag gaagtcattg
    24841 ttcagatcga taaagaagag cgcggcaaca aaggcgcggc attaaccacc tttatcagtc
    24901 tggcgggtag ctatctggtt ctgatgccga acaacccgcg cgcgggtggc atttctcgcc
    24961 gtatcgaagg cgacgaccgt accgaattaa aagaagcact ggcaagcctt gaactgccgg
    25021 aaggcatggg gcttatcgtg cgcaccgctg gcgtcggcaa atctgctgag gcgctgcaat
    25081 gggatttaag cttccgtctg aaacactggg aagccatcaa aaaagccgct gaaagccgcc
    25141 cggccccgtt cctgattcat caggagagca acgtaatcgt tcgcgcattc cgcgattact
    25201 tacgtcagga catcggcgaa atccttatcg ataacccgaa agtgctcgaa ctggcacgtc
    25261 agcatatcgc tgcattaggt cgcccggatt tcagcagcaa aatcaaactg tacaccggcg
    25321 agatcccgct gttcagccac taccagatcg agtcgcagat cgagtccgcc ttccagcgtg
    25381 aagtccgtct gccatccggt ggttcgattg ttatcgacag caccgaagcg ttaacggcca
    25441 tcgacatcaa ctccgcacgc gcgacccgcg gcggcgatat cgaagaaacc gcgtttaaca
    25501 ctaacctcga agctgccgat gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc
    25561 tgattgttat cgacttcatc gacatgacgc cagtacgcca ccagcgtgcg gtagaaaacc
    25621 gtctgcgtga agcggtgcgt caggaccgtg cgcgtattca aatcagccat atttctcgct
    25681 ttggcctgct ggaaatgtcc cgccagcgcc tgagcccatc actgggtgaa tccagtcatc
    25741 acgtttgtcc gcgttgttct ggtactggca ccgtgcgtga caacgaatcg ctgtcgctct
    25801 ctattctgcg tctgatcgaa gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca
    25861 ttgttcctgt gccaatcgct tcttacctgc tgaatgaaaa acgttctgcg gtaaatgcca
    25921 ttgaaactcg tcaggacggt gtgcgctgtg taattgtgcc aaacgatcag atggaaaccc
    25981 cgcactacca cgtgctgcgc gtgcgtaaag gggaagaaac cccaacctta agctacatgc
    26041 tgccgaagct gcatgaagaa gcgatggcgc tgccgtctga agaagagttc gctgaacgta
    26101 agcgtccgga acaacctgcg ctggcaacct ttgccatgcc ggatgtgccg ccagcgccaa
    26161 cgccagctga acctgccgcg cctgttgtag ccccagcacc gaaagctgca ccggcaacac
    26221 cagcaactcc tgcacaacct gggctgttga gccgcttctt cggcgcactg aaagcgctgt
    26281 tcagcggtgg tgaagaaacc aaaccgaccg agcaaccagc accgaaagca gaagcgaaac
    26341 cggaacgtca acaggatcgt cgcaagcctc gtcagaacaa ccgccgtgac cgtaatgagc
    26401 gccgcgacac ccgtagtgaa cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta
    26461 atcgtcgcca agcacagcag cagactgccg agacgcgtga gggccgtcag caggctgagg
    26521 taacggaaaa agcgcgtacc gccgacgagc agcaagcgcc gcgtcgtgaa cgtagccgcc
    26581 gccgtaacga tgataaacgt caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc
    26641 aatctgttca ggaaaccgaa caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac
    26701 agcgtcagct caatcagaaa gtgcgttacg agcaaagcgt agccgaagaa acggtagtcg
    26761 caccggtagc tgaagaaact gtcgctgccg aaccaattgt tcaggaagcg ccagctccac
    26821 gcacagaact ggtgaaagtc ccgctgccag tcgtagcgca aactgcacca gaacagcaag
    26881 aagagaacaa tgctgataac cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc
    26941 ctcgtcacct gcgcgtgagt ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa
    27001 cccaatcgcc aatgccgttg accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag
    27061 tctggatccg ctatccgatt gtacgtccac aagatgtaca ggttgaagag cagcgcgaac
    27121 aggaagaagt gcaagtgcag ccgatggtga ctgaggtccc tgtcgccgcc gctgtcgaac
    27181 cggttgttag cgcgccagtt gttgaagaaa tggctgaagt cgtagaagcc cccgtaccgg
    27241 ttgccgaacc gcaaccggaa gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcgg
    27301 taactgaaca gccgcaggtg attaccgagt ctgatgttgc cgtagcccag gaagttgcag
    27361 aacacgcaga accggtggtt gaaccgcagg aagagacggc agatattgaa gaagttgccg
    27421 aaactgctga ggttgtggtt gctgaacctg aagttgttgc tcaacctgcc gcgccagtcg
    27481 tcgctgaagt cgcagcagaa gttgaaacgg taaccgcggt taaaccagag atcaccgttg
    27541 agcataacca cgctaccgcg ccaatgacgc gcgctccggc accggaatat gttccggagg
    27601 caccgcgtca cagtgactgg cagcgcccta cttttgcctt cgaaggtaaa ggtgccgcag
    27661 gtggtcatac ggcaacgcat catgcctctg ccgctcctgc gcgtccgcaa cctgttgagt
    27721 aataattagc tcaagtaatc aagccctggt aactgccagg gcttttttat ttcatctttg
    27781 aatcaacata ttaaaattca tcgaatggca tccttgctaa ccaacaatgc aaaataggca
    27841 aaaaaatgcc cgccccggcg atccctgagc gggcagacaa aacataccca gtttcatgat
    27901 atgttttaaa gcgagtttat ttgttgagct ggaagagcga caatccctgc atatcggtaa
    27961 atgctttata cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat
    28021 tccagtccac atcaaccaga tcgctcatct gctgcgtttg ccctaatgcg cgatcgctac
    28081 ctaatgaatc cagcgactcc agttcgttca gctgcgtgcc taattccgcg cgaacggtca
    28141 ggacattgtt cagcgagttt ttcagtccgc ggttggtttt atctaacgcc gcagcggcgg
    28201 tttctttatc cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca
    28261 gcatggcaaa aagattggtt tcagaagcgc taccgtctgg ttccgctacc gcgttactgg
    28321 taatactgtc gaaaattttg tcgcccgtat gtcctatcac catcgaacgc gaagcatcga
    28381 cctgttgctt aatactttct gttccgccga cataatcacc gttaacctca ctaaacggcg
    28441 cagtctctgt tttataaccg gcaaaaatgt agcgcccgtt accgtcagtg gtgttcgcca
    28501 gattcagcaa ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcattgt
    28561 cactcaaggt gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcggtgg
    28621 tgacctggct aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgtgcca
    28681 gcgtgtactg gctgttttgc gcctgtgcct gggagagaac aacagcttgt gatgcagcaa
    28741 tgggatcgtc tgaagggtta acgactcgct tacccgtcga catctgctcg ccgtacttca
    28801 tccattctgc ctgagaattg gtgatgccgc gcatgttttg ctggtacatc atctgggtac
    28861 tgaaacgcat tttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc
    28921 cgtctgcaga acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta
    28981 ctcctcatcg agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac
    29041 attaccttgc gtggtgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac
    29101 taacgaggca taagcgtcgt taaaggattt cgctccgccc accgttttac tgttgctttg
    29161 cagatccagc agggcctgac cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc
    29221 catcgctatt ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg cgtcacttac
    29281 tggtttcagc gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc
    29341 atcaaatgcc actttaccgt tggcatctgg cgtcaccgta aaagtggtat tgctggcaag
    29401 gcgggtgacc tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccgc
    29461 ggaggcatca gttaccgtgg caccgatcgc aacgttaccg ttgtttttag tgttttgcag
    29521 aaccgcgggc ttaccgatag caaagaaatc ttcaccctca tcgccgttgg catcaaatcc
    29581 ggctttgtgt tggctgttga aagcctcagc aaatgccagc gccagttgtc caagcgtatt
    29641 acgcgtctgg tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt
    29701 cagtaatttc tccgggatct caatattgcc tgccgtccca tcaacataag cgacagtcgt
    29761 acgagaaggg tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac
    29821 cagtgagtaa ccattggcca tcgtgatgtt ataagtgccg ccatcctgaa cgctgacttc
    29881 tacaccaaca atctggttta attcgctcac cagttgatcg cgttgatcca gcagattgtt
    29941 aggtgacgcc cctgccccca cgcctgtcag gcgggagatt tgatcgttca ggctggcgat
    30001 ttgtttagcg taattgttga tctgatcaac gctggcacct atcgcgatat tgacctgttt
    30061 gtcctggtcg cgaagatact gatcggtggt tttaaactga ttcaccaacc cttctgattt
    30121 cccaatcagc gcctggcgcg ctgccgggtc ttccgcgtta ctcaccagcg tttgcaggct
    30181 ggtgaagaaa tcctgcatct gtgttgccag cgaagaggta ctggtggaga gcatattgtc
    30241 gattttcgac atctgctcat agcgggcagt caggccgcta ctttgcgtct gcgccgcacg
    30301 taactggttg gtaataaacg catcatactc acgctgcaca ccagaaacgt agacaccatt
    30361 accaacccag ccgccagcgc ccaacgtgct attggcctgc gccataatag tggtttggcg
    30421 ggtatatccg gcaacgttat agctggagat attattactt gccgtattta acgccgcctg
    30481 ggccgcgttc agtccgctca tggcgttatt gatcaagctg gacatggagg ttccttataa
    30541 gccttcaatt acagagtatt atcggcagcg acccgccgga cttgagttat tcagaacaga
    30601 ttatcaatgt tcatgctgta ggttttgctc accttgtcgc ttatcgattt catctgctga
    30661 atcatgttgg tgagtttacg ggcatagtga ggatcggtgg catagcccgc gtcctgtagg
    30721 gcctgcgccc cctgctccgc actcgcggcg gtcgtcacgg cggcgtagcg cgggttacgt
    30781 gttaacagcc caacgtaatc cgacaatgct tccagatacg agctgtagac ccgaaacttc
    30841 gcttttactt tcttcgcttc gccattttca tattcagtcg tggtgatttc agtgactggc
    30901 cctttccagt tgccagaggc tttgacacca aacaggttat agctcggctc gccgttttcg
    30961 cggcggattt gccgttgccc ccagccagat tccagcgccg cctgagcgag gatcaaatga
    31021 tgtggcacac cgctttgctg gcttgccagt tgggcgggca gcgagagttg cgcgaggaat
    31081 gctttactgt cacctggcag cgaatcatcg tagttacgtg gcacggcctt ttgcaccagc
    31141 tgcgaaagcg cctgattttg ataacgcacc acggtttcga gcgggaattt catcggtgct
    31201 gctggcgtgg actcctctgg caatggttgt tctggcgtca tctgcttaac catcatctct
    31261 gcaagcccca gacctttgcc cgtcgtcatc tgttgggcaa tctgctggtc atacatactg
    31321 gtatacaggc gagtgtgctc gctgctgaac aggccatctt ttggtaacgc gtcgcgcatg
    31381 cttttcaaca tcatctgcac gaacatccct tccacctgac gggccaccgg acggatattt
    31441 gccgccggat cttcgctcgc tttcgccttt agttcgttga gtgattgcgc atcccaggcc
    31501 gcacttgcca gtagtttgct gtcgctgatc atcagatgat ttccagtttt gcccgcagac
    31561 atcccgcact ttgcattgat tgcaaaatag acatcagatc catcggcgta gcgcccagcg
    31621 cattgagcgc gcgcaccacg ttattgaggc tggcgctgga acgtacgctt tgcagcgaac
    31681 cgccgctctg gcgtaagtcg atctgcgttt gtggcgttac cacggtctgt ccgccaccaa
    31741 acggtgtatc tggttggctg acattggcct gacgattgac tgttactgag agatttccct
    31801 gcgctatcgc gcagctgtcg agagtcactt cgcgattcat caccaccgaa ccggtgcgcg
    31861 agttaatcac tactttagcg tcctgcgggg tgacattaac ctgcatattc tggatatcgg
    31921 caaggaaacg gacctgggaa ctgttgccac tcggtacgcg cacctgaata gtccgcgcat
    31981 ccaacgcggt ggcgctgcca tatccacgca cgcggttgat ggtgtcagcg atttgctgcg
    32041 ccatgctgaa atcttcgtcg ttaagttgca aattaagggt attcccgacg ccaaactggc
    32101 tgggtaattc acgttcaata actgcaccat tggtgatccg tccaccgttc agttggttca
    32161 cctgaacact gctaccgcca gcggaggctc ctgcgccgcc aaccagaata ttgccctgcg
    32221 ccagcgcata cacctgactg tcaacgccct taagcggagt catcaacaat gtgccgccac
    32281 gcaggctttt ggcatttccc atggaagaaa ccaccacgtc aatggtttgc ccctgacgtc
    32341 caaacggtgg aagtgacgcc gtcaccatta ccgcagcaac gttttttagc tgcatattgg
    32401 tgcccgtcgg aacggtaatt cccagctgtg agagcatgtt attaagcgtt tgtgtggtaa
    32461 acggcgtctg ggttgtctgg tcaccggtgc catccagccc caccaccaga ccatagccaa
    32521 tcagtgagtt ttgccttacc ccctgaacac tggtgagatc gcgaatacgc tcagcctgag
    32581 ccgccgtcgt aaccagtaga agaattaatg cagagagaaa tttaatcacc acagcctcac
    32641 ttacattggc gacaggttaa ggaagaaacg ctgcaaccag cccatatttt gcgcttcgtt
    32701 aatgtagcca ttgcctacgt attcaatgcg cgcatccgcc acctgagtag acggtacggt
    32761 attgctgccg ctgatagtgc gtggattaac cacgccagag aagcgaataa attcggtacc
    32821 ctgattaatg gcaatctgtt tttcacccac cacatgcagg ttgccgttga ccagtacctg
    32881 gtcaaccgtc accgtcaacg tgccgctaaa ggtattactg gcattggccc cgccttttcc
    32941 gttgaacgtg ttaccaccgg aggcttcgac atcggcacga gcgttaccaa acagcccctg
    33001 caaatagcgc ggcacagtat caaagccaaa attagtttta ccgtcacggc tggcattcgc
    33061 agaggagctt ttgctggcgc taacgttctc ctgcaacacg atggtcagcg tatcgccaat
    33121 attgcgtggt cgacgatctt caaacagcgg ttgatagcca tagttaatcg gctgagcaga
    33181 ctggaaaata gaaccgttgg cgacgggcgt cggaccggga accggttgtg cactggtcgc
    33241 cccctgcacc agcggcgtgg agggtatcca ggcgcagccg gttagtgaaa gcaccaacaa
    33301 gctggaaatg gcataagtat gcgcagcgtt tttttgcatg gctatcatct tcaaaaatca
    33361 ataaaccggt gaacctgcca ccggttatgc cttagagttg cgtcagtttt tgcagcatct
    33421 gatcggtggt ggacaccgct ttactgttga tttcgtaagc gcgttgcacc tgaatcatat
    33481 tgaccagttc ttccgccacg ttgacgttag acgtttcaac atacccttga tacagcagtc
    33541 ccgcgccgtt caggcccggc gtgctttcgt tcggtgcacc ggaggattgc gtttcggtgt
    33601 agaggttttc gccaatgctc tccagcccgg tgtcattcat aaaggtggtg agattgagct
    33661 gcccaacctg aaccggagct gcctggcctt gttgggttac gctgaccacg ccatcacgac
    33721 cgatggtgat acttaacgca ttcgccggaa tggtgatcgc tggctgcacc tgaaaaccac
    33781 cagccgtcac cagctgcccg ttctgatcca cctggaaaga gccgtcacgg gtataggctg
    33841 atgagccatc tggcaacatc acctggaaaa agccctgccc tttaatcgcg acatctttac
    33901 tgttgttggt ctgcgacagg ttgccttggc tgtgtaagcg ttcagtggcg accgggcgta
    33961 cccccgtgcc gatttgtaat ccggagggta aggtggtttg ttcggaagac tgtgcccccg
    34021 gctggcgaat ggtttgataa agcagatctt caaacaccgc gcgctgacgc ttaaaaccgt
    34081 tagtactgac gtttgccagg ttgttggcaa tgacgtccat attggtttgc tgggcgtcaa
    34141 ggcccgtttt ggcgatccat aatgaactga tcataaaatg tcctgtagtg gattgctgac
    34201 aagcagagca gaagcacgcg gcggtgcgaa tttcccctca ccctaaccct ctccccaaag
    34261 gggcgaggga acagtccgct cgcttttatt gcaatgtcat caatcgttca attaactcat
    34321 cgacagcagt tggttggcac ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg
    34381 catttcaaaa cgccgcgcgc tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt
    34441 actgccttcc agaacccccg acatcacacg caaggttgga tctgcctgca gtaccggtcc
    34501 acgcgtggcc tgggtttctg cgcttaaacg aaaaatgccg tcgtcaccgc gctgcacttc
    34561 gctgcccgtg gctttcacca gtttcagacg ccccactggc gcaaccgtat ttgccggatc
    34621 gcccggattc agcgccgaga ttgttccatc ggcagctatc gtgatttccg ccccttccgg
    34681 cacagcaatt ggccccgcct cgcctatcac cggatgcccc tgaattgtca gttgcccggt
    34741 gggatcaacc tgaatgctgc cattacgcgt atacccttcg ctgccgtcag cggtctgcac
    34801 ggccaaccag ccatcctgct gcaacgcaac gtccagcggg cgcgaggtgt aatccatctt
    34861 gccgggcgtt atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga
    34921 aagcccttcc accggcactg cgcgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga
    34981 ggcattggcc agattactgg cggttaccgc ctgttgattc agcgtctggc tggctgctcc
    35041 catcgcggta taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa
    35101 ccagcgtgtt gaggatctgg tcctgggttt tgatggtctg ggcgttagac tgatagttac
    35161 gctgggcaac gatcatattg accagttctt tactgagatc gacgttggac gcttccaacg
    35221 caccgttggt cagggtgcca aagttgcccg tcccggctgt ccccaacagc gccacgccag
    35281 aagattgcgt cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa
    35341 agttcgccag tacaatctgc cccagcagtt gggtttgttc gttggaatag ttgccgacaa
    35401 ccgtaccgtc atcattgatt tgataactca ccagatcgcc cggtttgtag ccattctggg
    35461 tggttgccac aatgttgtta gcgccggtat tttgctgcat ggagttgagg aagctcaggc
    35521 taaacgtggc gggttctgcg ccgttaattg cgccggtggt aatattctct gttggatttg
    35581 aggtcagaac gccattggca ttaaacacca gcttcattgc aggctcggct gtacctgttg
    35641 gatcactgct atcctgggtg tagacctgcc agttattatc cccggtcttc acaaagtaga
    35701 cgctcatgtc atgagcatta ccctgactgt cgaaaacagt caccgaacct tttttgttat
    35761 agctatccgc attgctggca tcaaatgcgt taacagaggg aagcggatcg ctggaattca
    35821 ggttgatctg catcgacgcc gtggtggtag ttttcgctgc catcagggta ttcgggatcg
    35881 aaatgttagt cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac
    35941 ccgtcagctg taaaccttgc atattcacca ggttacgatt ttcatccagc ttaaattgtc
    36001 cgttacggct gtagaacacc gaaccgttgc tatctaccag acggaaaaaa ccgttctggc
    36061 tgatagcaac gtccagacca cgcccggtgt tggtggtcgt gccatcggta aagtcctgag
    36121 tgataccggc aacttttacc cccagtccca ctttcgaacc ggcaaacata tcggcaaaag
    36181 aggccgtgcc tgatttaaag ccgtaggtgg cggagttggc gatattgttg ccaataacat
    36241 cgaggttggt ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc atgactgact
    36301 cctgaaatgt gaaggcttaa attatctgcc gtacttcgtc gagggtggtg gtgccgtaag
    36361 tgccgagatc cagcgtatta ccgttgttgc cgcggatcac accctgcacc agagcaaact
    36421 gcagcggctg ggcaaccagt tgtgtaccac cgttactggc gctaatcgct acgttgtaag
    36481 aaccgttcgg cgcagtgctg ccgtcagtca acgtaccgtc ccaggtgaaa ctgtgaactc
    36541 cggcggtcag ttcaccaata tcaatggtgc gcacaaccgc gccattttta tcggtgatgg
    36601 tggccgtaac tttgtctgcc gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca
    36661 cagccccttc ttcactgccg gttcccgcaa gaacagtggt gccggggatc atcacgccgt
    36721 gaccgatcag gttactggcc tgtaacgact ggctgttatc aatctgtccg gaaatagatc
    36781 cgagcgtggt attgagtttt tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg
    36841 tcagctcgtt gttttccatt ggattggtcg ggtcctggtt tttcagctgc gccaccaaca
    36901 aagtcagaaa actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg
    36961 tggtactgac gccggtattt gtcggatcgg tggtggttac cacaatggac atagctttct
    37021 cctttattga ccgagcgtaa gggttttcag catcatgctt ttcaccgtgt tgagcacttc
    37081 aacattggcc tgatagctgc gtgacgccga catggtgtta accatctctc cgacaacatc
    37141 aacgttcggc atttttacgt agccctttgc atccgccagc ggattacccg gttcataaac
    37201 cagtttgtcc ggggcctgac tttctataac atcggcaacc tttacgccgc ctgtcgcagc
    37261 acctggtgca gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcagg
    37321 accggtcacg ctatcagcat tcgccagatt actggccgcc acgttcaggc gctgggactg
    37381 ggcagttaac gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc
    37441 gctctgtaaa acgttcatca tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg
    37501 gtattgcagg ctgttatcgg caaactgggt gcgttcgcga tccatatcga cggtattacc
    37561 gtcaagcgaa ggctggtccg gaatacggta ttgcagttct gcggtaggag gcgtcagcgc
    37621 ctgcgccgga atgtgttgcg ttgaggtcat cgtcagagca accacactgg ttgcattccg
    37681 tccacgttgc atgacttttt taagttcact ggcaaaatcg atatcgcgcg cctgataacc
    37741 aggggtatcg gcattggcga tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt
    37801 gagcgcctct tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca
    37861 ggtatcaaaa ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa
    37921 cgcaaaatgg gtcgctattt atgccgttga tggtcattgt ggacaggtac aattcacgtt
    37981 gtagaaatgg ctgggggcga aaatgctggc aataaaacgt agcgtggcga tcatcgcgat
    38041 actgttcagt ccgttaagtg cggcgagcaa tctcacatcg caattgcaaa ccttttttag
    38101 ctcccagctc gcagggataa gtgatgaggt tcgtgtttct attcgtacag cgcccaatct
    38161 actaccgccg tgcgagcagc cattgctttc gatgagcaat aattcccgct tgtggggcaa
    38221 tgtgaatgtg ttggcacgct gcggtaacga caaacgatat ttacaggtta atgtgcaggc
    38281 cacaggaaat tatgtggttg ccgcgatgcc cattgtgcga ggaggaaagc tgggagctgg
    38341 caatgtcaaa ctgaaacgcg gacggctgga taccttgcca ccgcgtacgg tgctggatat
    38401 caatcaactt gttgatgccg ttagcctgcg cgatctatca cccgatcaac ctatccagtt
    38461 aacccagttt cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaacg tgatcgccag
    38521 cggtgatggg tttagcgcca acgcagaagg tcaggcgcta aacaatgcag ccgtcgcaca
    38581 gaatgcgcgg gtgcgcatgg tatctggaca ggtagtcagc ggcgttgttg atgcagatgg
    38641 gaatattctt ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc
    38701 aacacatgat aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc
    38761 tctgaagccc gtaagcaccg ttcaaccgcg cgaaaccact gacgcgccgg taacgaacac
    38821 ccgagcggca aaaacaacag cctccaccag caccagtgtg acgttaagcg acgcgcaagc
    38881 aaaactgatg caacccggca gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact
    38941 ggcgattcgt aacggtgaac taaaaatgga caccggcaaa attgccgatg cgctgatcaa
    39001 cgaagcgcag caagacttgc agagtaatta atcgtatgac gcgtcttgca gagatcctcg
    39061 accagatgtc cgctgtgctt aacgatctca aaacggtaat ggatcaagag cagcaacatc
    39121 tctctatggg gcagatcaac ggcagccagt tgcaatggat tacagaacaa aaaagctcac
    39181 tgctggcaac gttggattac ctcgaacagt tacgcaggaa agaacccaat acagcaaata
    39241 gcgttgatat tagtcaacgc tggcaggaaa ttgctgtgaa aacgcagcaa ctacgccaaa
    39301 tgaatcaaca taacggctgg ttactggaag gacagattga gcgcaatcaa caggcgctgg
    39361 aaatgttgaa accgcatcag gaaccgacgc tatatggggc gaacggtcag acctcaacaa
    39421 cccatcgcgg cagtaaaaag atttcgatct gaagattatc tccggcctgc actgcaggcc
    39481 ggaatgcatt gttacaccgt ccggcgggca aattctttaa ctttgaagcc cagtaccgcc
    39541 agtgcagcga agtacgcggc aatccccgcc agcacgaccg ccattaaacg cagcaaacgc
    39601 cagggcatgg tacccaatga ccactccggc atgatatgta acatacctaa aagcacgcca
    39661 gacatcacca gtaccgccac caccagacgc aacagaaacg ccatccagcc aggttgcggg
    39721 gtaaagattt tctgcttacg caactgccag taaagcagcg aagcattcag acacgccgcc
    39781 agaccaatag aaagtgacag cccggcatgt ttcaacggac cgataaacgc caggttcatc
    39841 aattgcgtta aaatcagcgt aacgatggca attttcactg gcgttttaat gtcctggcgg
    39901 gaataaaagc caggagccaa cactttcact acaatcaggc caatcaaacc caccgagtag
    39961 gcaattaacg cccgctgggt catcagcgca tcaaaggcgg taaatttacc gtactggaac
    40021 agcgaaacgg ttaacggacc ggaaagaatg cccaacgcaa ccgcactcgg caacgccaac
    40081 aggaaacaaa gacgcagtcc ccagtccatc aaacggttgt attcatcatg attgccactg
    40141 gcaaaacttt tcgacagcga cggtagcaaa atggtgccaa gcgccacgcc cagcacaccg
    40201 gacggaaact ccattaagcg gtcggcgtaa tacatccatg acaccgaacc ggaagcaaga
    40261 aacgaggcaa aaatggtgtt gatgattaag gagatctggc tcacagagac gccaaggatt
    40321 gccggtccca tctgtttcac cacacgcatt gctccggcat cgtggaagtt aatgcgtggc
    40381 aaaaccagca tgccgatctt cttcagatgc ggtagctgat acaccagctg caggacgccg
    40441 ccgaccgtca cagcccacgc cagcgccagc accggtgggt taaagtacgg tgcggcaaac
    40501 agcgcgaaac caatcatgct gatgttaagc agtgttggag caaacgccgg aatcgagaag
    40561 cggttccacg tattcagaat cgctcccacc agcgacgcca gggagatcag caagatatag
    40621 ggaaaggtaa tctttagtag ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag
    40681 cctggcgcgg tcaccatgat cacccacggt gcggcgagca tgccagcgac cgtcacaacc
    40741 gccagcgcaa gtgtcagcag gccagaaaca taagagacaa agacccgcgt ggcgtcttca
    40801 ccctgcttac ttttatattc cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg
    40861 gcaaagatac ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc
    40921 atccctgcgc caaagattct ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa
    40981 aacatggtca tcgagctgac ggcggccagc gattttaata aattcatcgg tgttctaatc
    41041 cagacccacg ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg
    41101 attatggtga attgctaccg ccagatgtta cagggtttat tcactcatcg catcgcgcca
    41161 gatcttgtca acgatacgtt gcgccagcac tgcctgttcg ccggcggttt gcggaactgt
    41221 ctgattttgc acgcattcaa tgaagtgccg cgcacagccg acaaatccac gttgctcaag
    41281 cgtgctctgc caaccaggaa tcggtttatt cactacaccc tgcccacgct cctcacgcca
    41341 ttcgcgcatt tccgtaatgt cgatgagcgc accgtcagtc acggcctgca cggtttcacg
    41401 ctgacttccg gcacggcgat gcatacaagt ggtgatctgc aaaggaccgg ccgaaaaatg
    41461 gtgctcggca aacagcattt cgccagcgtc gttagtcagt agcgtaccgc catccagaga
    41521 ggctttgccg cccgacaacc acagcgcggt atccaccaca tgcagataat catccagcaa
    41581 cgtgaaataa agatcgtgtg gcccgacgct attgctacga tgtttatcca ttcttagcga
    41641 ggctgcggtg gcgagttgcg tttttaactc acagtagagt ggtgcgaaac gacggttaaa
    41701 accgaccatc aacgtcagtt ttttccgcgc cgccagttcc accagccgtt cagcatcgcg
    41761 cagattttct gccagcggtt tatcgacaca gacatgtacc cccgcattga gtaacgtact
    41821 gaccacgtca aagtgactgg cggtgctgga atgtacaaaa accgcatcgc aactggcggc
    41881 aaggctggat aacgaatcgg cataaggaat gcgccagctt tcacaaattg gcagggcttt
    41941 cgcgcgcgta ggcgaccagg ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg
    42001 taaccacgct ttttgcgcaa tgccgcctaa tcccactacg ccgatacgta attttttcac
    42061 tttaatctcc caggtgggcc agcaacgaat caagacgctg tttcagttct gccacttcga
    42121 tttccagggc ttcgacgcgg gcctgtaaat caccgtcaac cgcgttcgac atatccgtta
    42181 ccgccggctg atcctctacc tcaccactga aaagatgcat gtagcggctt tcgcgtttac
    42241 ccggttcgcg ggccagacgc accacaaaag gaccatcttc gcgattcgcc agttgttcca
    42301 gcgtcaactc cacttccgcc atatcgctga attcatacat tcgcgcggcg cggctgcgca
    42361 gttcacccgg cgtctgggca ccacgcaata acaacgtggt gattaacgcc acttctgcgg
    42421 cgctcagttt cagatcgcca aattctgaat tacaaaaacg ttgctcatat ttggtgaccc
    42481 gattaccaaa accgctcact gtgcgtagat aatgacgttt gaccagatta tccagctgtt
    42541 cctgcacttc agattcactc agattcatga ccggttcacg gttcgttttc tgattacagg
    42601 ccgtgactac accattgact gagagcgggt attgctccgg cgtggtcacc tgcttttcca
    42661 gcaagcaacc aatcacacga gcttccaggg cagtaagttg atatttcatg agattctcct
    42721 tagcggccgg gcgtccagtc tggggtagtt aatgccgtca gtacgtgatc gcgccattgt
    42781 ccatcaatca acagatagtc tttcgcatag ccttcttttt caaaacccag tcgcgccagt
    42841 aaatcaccgc tgcgtttatt gtgcggcata taattagcca taatgcgatg aatatgttgg
    42901 gtgcgctgca tataacgaat ggctgcggtc agggcttcaa acatgagtcc tttgccctgc
    42961 catttttgcc caatcgaata accgagatag caggcatgaa aagagccgcg aacaacattg
    43021 gaaaaattgg caacgccaat aatctctttt tcgtccgggt cgaataagcc aaagtagaaa
    43081 gctgaacctt gtttatgaaa ttcgttaatc atccccagcc tggcctgcca gcctgatgga
    43141 taacagtggc tttcgtcgcg cactggctcc cagggcttga ggaaatggcg attctctgcg
    43201 taataatccg ccagacgcca ggcatcacga tcatgcacca gacgcacgac cagtcggtct
    43261 gtggttaagc gcacttttgg cacgttactg cgatagccaa acattctata cctactcctt
    43321 cccgtaacgt ctttaatacg ctgtaagtat tactatacct gcgcaaccag accctgtgaa
    43381 aagtccaaaa tgccattttc tgcattattc acatatttga taaaaactct ctatcaaagc
    43441 gacgttttga taaaaaaata ttgtccccga agtgatggga aaagtcttag cggcaatgca
    43501 gaatagaaag tgctcatctt ttatttcccg ggaggggaaa tgtcccgcgt gtcgcaggcg
    43561 aggaacctgg gtaaatattt cctgctcatc gataatatgc tggtcgtgct ggggttcttt
    43621 gttgtcttcc cgctgatctc tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg
    43681 gtcggtattg ccctcggcct acgccagttt attcagcaag gtctgggtat tttcggcggc
    43741 gcaattgccg accgctttgg tgccaaaccg atgattgtta ccggtatgct gatgcgtgcc
    43801 gccggattcg ccacaatggg tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg
    43861 ctctcgggac tcggtggcac gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta
    43921 atccgtccac aacagcgtgg tcgttttttc tcgctgttga tgatgcagga cagtgccggt
    43981 gcggtcattg gcgcattgtt ggggagctgg ctgttgcaat acgactttcg cctggtctgc
    44041 gccacagggg ccgttttgtt tgtgctgtgt gcagcgttca atgcgtggtt gttaccggca
    44101 tggaaactct ccaccgtacg cacgcccgtt cgcgaaggca tgacccgcgt gatgcgcgac
    44161 aagcgttttg tcacctatgt cctgacgctg gcgggttact acatgctggc tgtacaagtg
    44221 atgctgatgc tgccaattat ggtcaacgat gtggctggcg cgccctctgc cgttaaatgg
    44281 atgtatgcca ttgaagcgtg tctgtcgtta acgttgctct accctatcgc ccgctggagt
    44341 gaaaagcatt ttcgtctgga acaccggttg atggctgggc tgttgataat gtcattaagc
    44401 atgatgccgg tgggcatggt cagcggcctg caacaacttt tcaccctgat ttgtctgttt
    44461 tatatcgggt cgatcattgc cgagcctgcg cgtgaaacct taagtgcttc gctggcggac
    44521 gcaagagctc gcggcagcta tatggggttt agccgtctgg gtctggcgat tggcggcgct
    44581 attggttata tcggtggcgg ctggctgttt gacctgggca aatcggtgca ccagccagag
    44641 cttccgtgga tgatgctggg cattattggc atcttcactt tccttgcgct gggttggcag
    44701 tttagccaga aacgcgccgc gcgtcgtttg cttgaacgcg acgcctgatt tgatactccc
    44761 ccctgactcc tccatactga aagtgaactg acagccaaat ggaggagtca cgtgaagcta
    44821 tacatttacg atcactgccc ttactgcctc aaagcccgca tgattttcgg cctgaaaaat
    44881 atccccgtcg aattacatgt tctgctcaac gacgacgcag aaacacccac ccggatggtc
    44941 ggtcaaaaac aggttcccat tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg
    45001 gatatcgttc actatgtcga taaactcgac ggcaaaccgt tactgaccgg caaacgttcc
    45061 cctgccattg aagagtggct gcgcaaggtc aatggctacg ccaacaaact gctgttgccg
    45121 cgttttgcca aatcggcatt tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc
    45181 gacaagaaag aggccagcgc gggtaatttt gccgacctgc tggcccactc tgacggtctg
    45241 attaagaata tcagcgatga tttacgtgcg ctggacaaac tgatcgtcaa accgaacgcc
    45301 gtgaatggcg aactttcgga agatgatatt cagctattcc cgctactgcg taatctgacg
    45361 ctggtagccg gaattaactg gccaagccgc gttgctgatt accgcgataa tatggcgaaa
    45421 cagacacaaa tcaatttgtt atcatcaatg gcgatttaat cctgactcgc cgcggcagcc
    45481 tgtattcgcg ggttgccgcc gttttcagtt ttttctgctg gcgagtttat cgctggtgac
    45541 acatgctgcg ttgtgtcagg atattgacgc agatttcatg tcgaggacta ccatgaacaa
    45601 gtttttattt gccgctgcat tgatcgtcag tggcctgctc gttggctgta atcaactcac
    45661 ccaatacacc atcaccgaac aagaaattaa ccagtcgctt gcgaaacata ataatttctc
    45721 aaaagatatt ggtttacccg gtgtggctga cgcccatatt gttctgacaa acctgaccag
    45781 ccaaattggt cgcgaagagc cgaataaggt tgccctaacc ggagacgcca atctggacat
    45841 gaactccctg ttcggtagcc agaaagcgac catgaaactg aagctgaaag cgctgcccgt
    45901 gtttgataaa gaaaaaggcg cgatcttcct gaaagagatg gaggtggtcg atgcgacggt
    45961 acaaccggaa aaaatgcaaa cggtgatgca aacgttgctt ccctatctga accaggcatt
    46021 acgcaattac tttaaccagc aacctgctta cgtcctgcgc gaagatggca gccagggcga
    46081 agcaatggcg aaaaaactgg cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc
    46141 atttactgat taatcacgag ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg
    46201 tttccgctta tcctttgtgt ccggcaaaaa catcccttca gccggagcat agagattaat
    46261 gactgcacca tcccaggtat taaagatccg ccgcccagac gactggcacc ttcacctccg
    46321 cgatggcgac atgttaaaaa ctgtcgtgcc gtataccagc gaaatttatg gacgggctat
    46381 cgtaatgccc aatctggctc cgcccgtgac caccgttgag gctgccgtgg cgtatcgcca
    46441 acgtattctt cacgccgtac ctgccaggca cgatttcacc ccattgatga cctgttattt
    46501 aacagattcg ctggatccta atgagctgga gcgcggattt aacgaaggcg tgttcaccgc
    46561 tgcaaaactt tacccggcaa acgcaaccac taactccagc cacggcgtga cgtcagttga
    46621 cgcaatcatg ccggtattag agcgcatgga aaaaatcggt atgccgctac tggtgcatgg
    46681 tgaagtgaca catgcagata tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt
    46741 gatggaacct ctgcgccagc gcctgaccgc gctgaaagtc gtttttgagc acatcaccac
    46801 caaagacgca gccgactatg tccgtgacgg aaatgaacgg ctggctgcta ccatcactcc
    46861 gcaacatctg atgtttaacc gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta
    46921 ttgtctaccc atcctcaaac gcaatattca ccaacaggca ttgcgtgaac tagtcgccag
    46981 cggttttaat cgagtattcc tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga
    47041 gagcagctgc ggctgcgcgg gctgcttcaa cgccccaacc gcgctgggaa gttacgctac
    47101 cgtctttgaa gagatgaatg ctctgcagca ctttgaagca ttctgttctg taaacggccc
    47161 gcagttctat gggttaccgg tcaacgacac attcatcgaa ctggtacgtg aagagcatca
    47221 ggttgctgaa agcatcgcac tgactgatga cacgctggtg ccattcctcg ccggggaaac
    47281 ggtacgctgg tctgttaaac aataaaaatc gtagcttcct gttgtcatta ggttatttta
    47341 cctgtataaa taaccagtat attcaacagg gggctattat gcgaattgaa gtcaccatag
    47401 cgaaaacttc tccattgcca gctggggcta ttgacgccct ggctggcgaa ctttcccgcc
    47461 gtattcagta tgcgtttcct gataatgaag gccacgtatc agtacgttat gccgcagcga
    47521 ataatttatc ggttattggc gcgacaaaag aagataaaca gcgcattagc gaaattctcc
    47581 aggaaacgtg ggaaagcgcc gatgactggt ttgtcagcga ataatatgca gtgatttttg
    47641 tttttgccgg gtcgccccgg cttttttatt ttacttgcga attatgagta caaaatttct
    47701 acacttcctc aaaataattg cacaatatgc taaaaaaata tgaatgacat gctgttttat
    47761 tgttcatata aatcttaaaa gcagaaattt atctgttccc ccctttaaaa attcggtgat
    47821 tacccttatt tattggtata ctgaagttgc ttcagataaa aacgcattga acctcgaata
    47881 acgttgtcta gtaacacgaa ttagggggcc atgatggaaa aaaataatga agtcattcag
    47941 actcatccgc tcgtagggtg ggacatcagc accgttgata gctatgatgc gctgatgttg
    48001 cgtttgcact accagacccc aaataagtcc gagcaggaag ggactgaagt tggtcagacg
    48061 ctctggttaa ccactgatgt tgccagacaa tttatttcga tattagaagc aggaatcgcc
    48121 aaaattgaat ccggtgattt cccggtaaac gagtatcggc gtcattaaca tgacatgacc
    48181 atccacccaa aaggcacctc accggtgcct ttaccatttc tgggttgtat atctgcattc
    48241 agttaattac tctgcttaaa ccatgagagc agagagaaaa aaatgaaata cgatctcatc
    48301 attattggca gcggttccgt aggcgctgcc gccgggtatt atgcaacccg cgccggttta
    48361 aacgtgctta tgaccgacgc ccatatgcca ccacatcaac acggcagcca ccacggcgat
    48421 acgcgattaa ttcgacatgc ttatggtgaa ggcgaaaagt atgtcccgct ggtcctccgc
    48481 gcacaaacgc tgtgggatga cctctcccgc cacaacgaag atgatcccat ttttgtacgc
    48541 tctggtgtca ttaaccttgg cccggctgac tccgcatttc tcgccaacgt cgcccacagc
    48601 gccgaacagt ggcaactcaa cgttgaaaag ctcgatgcgc aagggattat ggcccgctgg
    48661 ccagaaatac gcgtcccgga caactacatc ggcttatttg agactgattc cggttttttg
    48721 cgcagcgaac tggcgattaa aacctggatc caactggcga aggaagcggg ctgtgcgcaa
    48781 ctgttcaact gcccggtcac cgcaattcgt catgacgatg atggcgtaac tattgaaacg
    48841 gctgacggtg agtatcaggc gaaaaaagcg attgtctgcg cgggaacatg ggtaaaagac
    48901 ctgctcccgg agctgcctgt ccagcccgta cgtaaagtat ttgcctggta tcaggccgat
    48961 ggccgctata gcgtgaagaa taaattcccg gcgtttaccg gtgaactgcc caatggcgat
    49021 caatattatg gttttccggc agaaaacgac gcgttgaaga ttggcaaaca taacggaggc
    49081 caggttatcc attcagcgga tgaacgtgtt ccgtttgcgg aagtggtcag cgatggttcg
    49141 gaagccttcc cgttcttgcg caatgtattg ccgggtatcg gttgctgcct gtacggcgct
    49201 gcctgcacct atgataattc gcctgacgaa gattttatta tcgataccct acccggccac
    49261 gataatacac tgctcattac cggcctgagt gggcacggtt ttaaatttgc gtcagtttta
    49321 ggggaaatag ctgccgattt tgcgcaagac aaaaaaagcg attttgattt gacgccattc
    49381 agcctttccc gcttccaata atcatcatta cggcctccag aatgaggccg tttttacagg
    49441 agtatttaat gcgtcggctg ttgcactatc tcatcaataa tattcgcgag catctgatgc
    49501 tatatctttt tctctgggga ttgttggcaa ttatggatct gatctatgtg ttttattttt
    49561 agttttccgc actgacataa actgacaaat atttaatctc cctttcattt agcgtgaaaa
    49621 attgaatgag attattcctg cttctacagc actcaccaga catctctgat gctgaaccca
    49681 ctaaacttat gaagattctt tcattattaa ttcaaaatta ttgaaataaa aataaatatc
    49741 attcaatttg ttatttttgt gttgcaattc attctccgac acgccatttt ctttctctca
    49801 caaattcgtt ttggatgcac ttatgtcatt cacaaatacc cctgaacgct atggtgttat
    49861 atctgcggcc ttccattggc taagcgcaat tattgtctac ggcatgtttg ctttaggtct
    49921 gtggatggtc acgctcagtt attacgatgg ctggtatcac aaagcacccg aactacataa
    49981 aagcatcggt attttgttaa tgatggggct ggttattcgc gttctgtggc gtgtcatatc
    50041 tccaccgccg ggtccgctgc cgagttattc gcccatgact cgtcttgccg caaaagctgg
    50101 acatctcgca ttgtacctat tgctgttcgc catcggcatc agcggctatc tgatctcaac
    50161 tgccgatggt aaaccgatca gcgtttttgg ctggtttgac gtccccgcga cccttgccga
    50221 cgctggcgca caggcagact ttgccggtgc cctgcatttt tggctcgcgt ggagcgtcgt
    50281 agtactgtcc gttatgcacg gatttatggc cctgaagcat catttcatcg ataaagacga
    50341 cactctgaag cgcatgctgg gaaagtcgtc atctgactat ggagtataaa aaatgaaaaa
    50401 aagcctgctt ggtttaacct tcgcgtccct gatgttctct gccggttcag cggttgccgc
    50461 cgattacaaa attgacaaag aaggtcagca cgcctttgtt aactttcgca tccagcatct
    50521 tggctatagc tggttatatg gcacctttaa agatttcgac ggtactttta cctttgatga
    50581 aaaaaatcca gctgccgata aagtgaatgt gacaattaac accaccagcg tcgatactaa
    50641 tcacgccgaa cgtgataaac atcttcgcag tgcagatttc ctcaataccg caaaatatcc
    50701 acaggcaaca ttcacctcca ccagcgtgaa gaaagacggt gacgaactgg atattaccgg
    50761 cgatctgacg ctgaatggtg taaccaaacc cgtcacgctg gaagcgaaat taattggtca
    50821 gggcgacgac ccatggggtg gtaaacgtgc aggcttcgag gccgaaggca aaattaagct
    50881 caaggacttc aatatcaaga cagatttagg tccagcttct caggaagtgg atctgattat
    50941 ttcagtggaa ggcgtacagc agaagtaaga gtgaaatgtt gccggatggc acgcatccgg
    51001 caatgatatt tattctgttg gatcaggaat gcccagtgtt gtattcagac gtccacgaga
    51061 cttattaaag atcttattgc cattttcacg tcctgcccga cggcgtcgct gttcttctgg
    51121 cggtaacgcg ctttcttcgc agcaaatttc actacaacaa cctttgtatt tttccgcaca
    51181 tactggacac tgaataaaca gcaggtggca gccatcattt ttacagttgg tatggctgtc
    51241 gcacggcgca ccgcactggt ggcaatgcgc gataatctca tcagatatac gttcgcccat
    51301 ccgctcgtca aaaacaaaat ttttgccaat aaaacgcacc ggcaagccct gctcgcgcgc
    51361 cttacgggcg tattcaataa ttccaccctc gatatgccag actttattga atccgttatg
    51421 tttcatccag gcactggcct tttcacaacg aatgccgccg gtgcagtaca tgacgatttt
    51481 tttatcttta tgtgcctgca tcatctcgac tgcttttggc agctgctcac ggaaggtatc
    51541 tgccggaatt tccagcgcgt tttcaaagtg ccccacttca tactcatagt ggttacgcat
    51601 gtcgataaac agtgcatcgg gatcgtcaag catggcgttc acttccgccg cttgcagata
    51661 ctcaccaaca ttgctggcat caaagtgagg atcgtcaata ccgtcggcaa cgatacgatc
    51721 gcggaccttc atgcgcagta cccagaagga tttcccgtca tcatccaacg cgatattcag
    51781 gcgtaagcca tctaaagccg agtcgaaggc atagagctgc gcgcgaaatg ttttaacatt
    51841 gctcgcaggt acgctgattt gcgcgttaat gccctcatgc gccagataca ctcgcccaaa
    51901 aacattcagc gcggtaaaca gctgatataa agcgtcacgg gtcgccttag gatcggcgat
    51961 gtggaaatac ttgtaaaacg aaatggtggt tcgcggttcg ctctcagcca acattttggc
    52021 ttttaacgcg tcgttggaaa tgcggttgtg taacactggc atggtgtacg gttcctgcga
    52081 gatgggaaag taaaaatccg cggcatgata tagcaattat cgataattaa catccacaca
    52141 ttttacgcta catttgcgca ttaaaaatta tttgttattt acaagcgcgg caatttcgcc
    52201 cagtcttcag ccacaaattt tggttgcggg cgaaaaaatg cgacaataca tacaattgcc
    52261 cgaataggtt gaaaaacagg attgatatga cgaatctacc caagttctcc accgcactgc
    52321 ttcatccgcg ttattggtta acctggttgg gtattggcgt actttggtta gtcgtgcaat
    52381 tgccctaccc ggttatctac cgcctcggtt gtggattagg aaaactggcg ttacgtttta
    52441 tgaaacgacg cgcaaaaatt gtgcatcgca acctggaact gtgcttcccg gaaatgagcg
    52501 aacaagaacg ccgtaaaatg gtggtgaaga atttcgaatc cgttggcatg ggactgatgg
    52561 aaactggcat ggcgtggttc tggccggacc gccgaatcgc tcgctggacg gaagtgatcg
    52621 gcatggaaca cattcgtgac gtgcaggcgc aaaaacgcgg catcctgtta gttggcatcc
    52681 attttctgac actggagctg ggtgcgcggc agtttggtat gcaggaaccg gggattggcg
    52741 tttaccgccc gaacgataat ccactgattg actggctaca aacctggggc cgtttgcgct
    52801 caaataaatc gatgctcgac cgcaaagatt taaaaggcat gattaaagcc ctgaaaaaag
    52861 gcgaagtggt ctggtacgca ccggatcatg attacggccc gcgctcaagc gttttcgtcc
    52921 ctttatttgc cgttgagcag gctgcgacca cgaccggaac ctggatgctg gcacggatgt
    52981 ccggcgcatg tctggtgccc ttcgttccac gccgtaagcc agatggcaaa gggtatcaat
    53041 tgattatgct gccgccagag tgttctccgc cgctggatga tgccgaaact accgccgcgt
    53101 ggatgaacaa agtggtcgaa aaatgcatca tgatggcacc agagcagtat atgtggttac
    53161 accgtcgctt taaaacacgc ccggaaggcg ttccttcacg ctattaaatc tcccatgccg
    53221 gatgcttcag aatggcatcc ggcattacca cagcaaatcc ccctgattta gcgataaaag
    53281 ctctctggat tgcgccccct ggaagtcggg cgcataatta gtgtgcttat cttttcttct
    53341 tatgttcacc gcgcctggcg caccaacagc ggattgctat gtcaccctgt gaaaatgaca
    53401 cccctataaa ctggaaacga aacctgatcg tcgcctggct aggctgtttt cttaccggtg
    53461 ccgccttcag tctggtaatg cccttctaac ccctctacgt tgagcagctt ggcgttaccg
    53521 gccactccgc cctgaatatg tggtccggta ttgtcttcag cattacattt ttattttcgg
    53581 ccatcgcctc accgttttgg ggtggactcg ccgaccgtaa aggccgaaaa ctcatgctat
    53641 tacgctctgc tctcggcatg ggcatcgtga tggtgttgat ggggctggca caaaatatct
    53701 ggcagttttt gatcctgcgg gcgcttcttg ggttacttgg cggatttgtc cccaacgcta
    53761 atgctcttat cgccacacaa gtaccgcgta ataaaagcgg ctgggcactg ggtacgctct
    53821 ccacaggcgg cgttagcggt gcgttgctcg gcccaatggc tggcggcctg ctcgccgata
    53881 gctacggctt acgtccggta ttctttatta ccgccagtgt gctcatactc tgctttttcg
    53941 tcaccctgtt ttgcatcaga gaaaaattcc agccggtcag caaaaaagag atgctgcaca
    54001 tgcgggaagt ggtgacatca cttaaaaacc cgaaactggt actcagcctg tttgtcacca
    54061 cgttaatcat ccaggtggcg acgggctcaa ttgcccccat tctgacgctg tatgtccgcg
    54121 aactggcggg taacgtcagt aacgtcgcct ttatcagtgg catgatcgcc tcggtgccag
    54181 gcgtagctgc tctgctgagt gcaccacgac tcggcaaact tggcgatcga atcggacccg
    54241 aaaagatcct gattacggcg ctgatctttt ctgtactgct gttgatccca atgtcttacg
    54301 ttcagacgcc attgcaactt gggattttac gttttttgct cggtgccgcc gatggtgcac
    54361 tactccccgc cgtacagaca ctgttggttt acaactcgag caaccaaatc gccggacgta
    54421 tcttcagcta taaccaatcg tttcgtgata ttggcaacgt taccggacca ttgatgggag
    54481 cagcgatttc agcgaactac ggtttcagag cggtatttct cgtcaccgct ggcgtagtgt
    54541 tattcaacgc agtctattca tggaacagtc tacgtcgtcg tcgaataccc caggtatcga
    54601 actgattttt cgcctttcat acttgcaaaa gcggagaatc agctatcctt ttccctgaaa
    54661 cctcatcaac tcaaagggag aatcgtgatg accatgtacg caacgcttga agaagccatt
    54721 gacgctgcac gcgaagaatt tcttgcagac aaccccggca tcgacgccga agatgcgaat
    54781 gtgcaacagt tcaatgccca aaaatacgtt ttgcaggacg gcgacatcat gtggcaagtt
    54841 gagttttttg ccgacgaagg ggaagaaggt gaatgtttac ctatgcttag cggtgaagcc
    54901 gcgcaaagtg tttttgatgg cgactatgat gagatagaga tccgccagga gtggcaggaa
    54961 gagaatacat tacatgaatg ggacgagggg gaatttcagc ttgagccacc gctggatacc
    55021 gaggaaggac gcgcagcagc tgatgagtgg gatgaacgtt aattactcat acgggccatg
    55081 atgaatgtcg atcggcagca gtaaagtatc aaaaaccagg gagaatggca gatcaaggat
    55141 tgtgacataa cgccaggcgg agtcacgcac atcccattgc acaccgggat aatattggtt
    55201 gccatgcccc tgccccggta tagtgcggct aatgatgctg ccacagccac tcagcaaggt
    55261 caccatgatg ctcaccacaa ttaaacgcat ttgatacctc ttccgtaaaa aaataccggc
    55321 ataacgccgg tatttttatt ggctcgttgt ttttgccaga tgtggcgtga acgccttatc
    55381 cgacctacaa aatcttgcaa aatcaacaca ttgcaggagc gatgtaggcc tgataagcat
    55441 agcgcatcag gcaactacgt ttttattgcg aagccgcatc cggtttacgc aatgccagtg
    55501 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
    55561 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
    55621 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacatggcgg tcacgggcga
    55681 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
    55741 atgacgggtt aaacactgcg tgcataaaac catcatcaag ggagcgttga cggttcatct
    55801 caaggaaccg atcggtatca accagtacct gcggcggtga atactcttcc gggatcagga
    55861 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
    55921 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
    55981 accacgccat cccgacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
    56041 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
    56101 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
    56161 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccatatt
    56221 ctttcgttcc tttacaccaa ataagcaaaa tgctcaatag ctttggcaga aacaacagca
    56281 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
    56341 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgcact acctgcaatg
    56401 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
    56461 tcaggaatac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
    56521 tcaggttacc gtggcaccag cggcggtcac gttttagctc atcaagcaag ttaggcggta
    56581 attcttcata agaacccggg agatcgtaag caatccagac tccccaacct gcacggcgca
    56641 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
    56701 gcagcggggc cagtgcacag tgctcgataa acggtttcac gcggataatc gcgttatgcc
    56761 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat
    56821 acacgcgggt cgcgaactgc tgacagcgtg catacagcgt atccataccg gacgctttcg
    56881 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc aggccgcaca
    56941 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catatagctg tactggctgc
    57001 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcga cggcggcggc
    57061 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
    57121 gctctgcgac gcagatatcc gggttgtaac tgtcactcag aatgtagaca tcaaagtgtt
    57181 tggcattccc ggtggctttt actgattccc acgttgcacg caagccagca aaaacacggt
    57241 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
    57301 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagt agttgcagga
    57361 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
    57421 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
    57481 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
    57541 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
    57601 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
    57661 gatagcgcgg tgttacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
    57721 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttaagctgat
    57781 cgcgcccttc gtcatcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
    57841 ccagacgcgc ctttaccgag ccttgcgggg agtcatcctc ccgcgcccag gtgcggtgtt
    57901 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
    57961 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
    58021 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcgcg catttcagtg
    58081 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtgaccggg
    58141 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
    58201 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
    58261 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
    58321 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
    58381 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
    58441 gcgacgatgt tatcgttagt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
    58501 ttaccccact cccctttcgg agtcacccat gcgcttggac ggagatcgta acgatcatcg
    58561 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccaaagcc ttgcgggttt
    58621 tccatggaga agctgctgac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
    58681 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
    58741 tttgctggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
    58801 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
    58861 cctggcataa ctacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
    58921 atggttaaac gtttatcagt tggttttgga cgctcgatcc agaactcttt gaagcgtgga
    58981 aattcttcac ccgatggcaa ggcggtatca attgccagac cgcgggcaga aaggccataa
    59041 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
    59101 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
    59161 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
    59221 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
    59281 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
    59341 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
    59401 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
    59461 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
    59521 cgcattttca tcatttgtag tttatgtttc ataattagta agcacttccc cctttgtgtg
    59581 cttatatcga tccgatctat tttaatggat aaccagccat tccgacaaca gaatcccggt
    59641 atcgttcagc acgctggctc atggattaag cacctgattg caaccgtttt cactttccgt
    59701 gcttctccta aagacagaag catcgacttc gtgtagggtt tgccagcaaa tttatttatc
    59761 aaccgtctta cagacgcttc cattctggtt agtggtagcg atgatgctat gcataaaaac
    59821 ttgaagcctg cgcctggcgc ggtcaggtcg gtgtcgctat ggcaacaagc ctaaaggcat
    59881 aacccgacag ggataagacg aaaaatccga gattagttaa ccatatatga atccagtacc
    59941 cgcgcaacgt gaatatttcc tcgactccat ccgcgcctgg ctgatgttgt tagggatccc
    60001 ttttcatatt tctttaatct attcgagcca tacatggcat gtgaatagcg ccgaaccgtc
    60061 attgtggctg acccttttta atgacttcat ccactcgttc cgcatgcagg tatttttcgt
    60121 tatatccggg tacttttcct acatgctttt tttacgctat cccttgaaaa aatggtggaa
    60181 agtacgtgtc gaacgtgtag ggatcccgat gttaacagcc atccccctac tgacattgcc
    60241 gcaatttatt atgctgcaat acgtcaaagg gaaagcggaa agttggcctg ggctgtcatt
    60301 gtatgacaaa tataatacgt tggcctggga attaatatca catctgtggt ttttactggt
    60361 gttagtggtc atgacgacgc tgtgcgtatg gatatttaaa cgcatcagaa ataatttaga
    60421 aaattctgat aaaacgaata aaaaattctc gatggtaaaa ctatcggtga tttttttgtg
    60481 cctcggcatc ggttatgcgg taataagaag aacgattttt attgtgtatc cacccattct
    60541 gagtaatggc acgttcaatt ttattgtcat gcaaacgcta ttttatttac cgttctttat
    60601 cctcggcgca ctggctttca ttttccctca tcttaaagcc ttgtttacca cgccgtctcg
    60661 tggctgtacc ctcgctgcag cattggcgtt tgtcgcttat ttactcaacc agcgctatgg
    60721 cagtggcgat gcctggatgt acgaaaccga gtcggtgatc accatggtcc tcggtctgtg
    60781 gatggtgaat gtggtcttct cctttggcca ccgtttgctt aacttccagt cagcgcgggt
    60841 gacttatttt gttaacgcat cgctgtttat ctatctggtt caccacccgt taacgctgtt
    60901 tttcggcgca tacattacac cgcacatcac ctccaactgg cttggttttc tctgtggcct
    60961 gatattcgta gtagggattg cgataattct gtatgaaatt catttgcgca tcccgttact
    61021 gaagtttttg ttctctggta aaccggttgt taagcgtgag aacgataaag caccagcccg
    61081 ttaagccaca tttacaataa ccattccacg ggcaatatcg acgccagtct gaccataacc
    61141 cgcttccaga aactggtagc gggttctttt ttgagaacaa tctctttctt tgcatgacga
    61201 tcaacccagt tgatccgtcc ccacctgtcc agacggagct gccaggccgc atcatactgg
    61261 ctctgaataa agcgtttatc aattaactga gccagcgttt cgctctctat cacgaagccc
    61321 atttcagtat tgagcaatgt tgaacgcgga tcgaaattga aagaaccgat aaacaccgtt
    61381 ttaccatcga tgctaaaggt tttagcatgc aggctggcac cggaattacc ggttatgccg
    61441 cgatcgtgta acgtactact ttgttcacgc gtcggcttga gttcatataa ttccacgcca
    61501 tagcggagca attttttgcg ccagcgcgcg tatccggcat ggacgacagc aacatcgtta
    61561 gcggcaagag aattggttag gatcgcaatc tttacccctt ttctcaccat ccgtaagagt
    61621 tgcgccacac ctgcgcgtgt cggtacaaaa taggaagaga taatatcgat gcgttcactg
    61681 ggtgagccca tgatatcgaa caggcgctgc ggtaacagtg aatgccgttt tgccttgccc
    61741 tcccctttcg ccggatcatc acttaataaa cgtgttttcg cccagataag cggcaacgtt
    61801 ccatcaacca gatgatttat aaacggactg gattccattt tgcgtaagta acgatgcgtc
    61861 atggcatcgt tatgccagga ggcgggtaac tcgatgcgat ccgccatttc accttccggg
    61921 acatccagca cctgctgtaa gggtgaaacc gatttgcaat accagtagcg ggcgaaatca
    61981 tcggcaacgt cctctactac gggtcctatt gccatgacat ctaaatccga aaaaagtggc
    62041 tcttcccctg ccccaaaata ggcatcacca atatttcgcc cccctaccaa cgtcaccacg
    62101 ccatcgacag tgaaactttt attgtgcata cggcgattga gacgggaaaa gtcggtgata
    62161 taaccaagcg gacgcagcaa gcgaaacgag aaaggattaa aaagccggac ttcaatgcgg
    62221 ggatggctgt caagcaagcg taaaatgtcg tcaagtccgg gcgtattgtt gtcgtccagc
    62281 aacaaacgga cgcgaacgcc acgctttgct gcggctaaca gggcggaaaa cagtaaccga
    62341 cccgacatat cgtcctgcca gatgtaatac tgaacatcca gcgtatgctc tgacatttcg
    62401 gccagacgat accgggcggc aaacgcatcc agacttttct ccagagggaa aagaccacac
    62461 tgaccgggat gttgcgaaca cagtggcagc accgcgctcg ccagccgggg caaatcattc
    62521 atctccttgt tgggtaagga gtctttcgta gaggtgggcg ttttcttcat cataacagac
    62581 aaagtatacc tgttcgggta aagcgtgacg ggtaataaat tctgaaacgg tttttactgc
    62641 gatttcagcc gctgccgcac gagggtaacc ataaacccca gtactgattg caggaaaagc
    62701 cactgacgta tagctgtttg ccgccaccag tcgtaggcta ttgagatagg catcctgcaa
    62761 aagctggtct tcgttttgtt caccaccacg ccagactggc ccgacggtgt gcactacggc
    62821 tttagcagga agatcgcctg caagcgtaat aacggcatgc cccgtagggc aatcgccctg
    62881 ctgttgcctg acttttaaac aagcatccag cagggccgga cccgctgcgc gatgaatggc
    62941 cccatcgacg ccgccacctc ccattaatga aggattagcc gcattcacaa tcacatcaac
    63001 ggccagtttg gtaatatcac cctgcacaac atgaatacgc gttttcataa cgcctcctta
    63061 caccttcttg tttcttaagt gtacagcagg ctttttggtt aaatcgcatt tttctgctca
    63121 gcaacgtgag ttgttcagaa acttagttat attcgatgtt cattacagcc agctttttct
    63181 gtttatcaag atactgcact tttactgagg ctatttgcgc gtttttgaca ttgccgctca
    63241 ttaacgcttt gctggagtag gttttacccg gaacagaacc attctgtggg cagttcattt
    63301 ctacgggtga tgactgggtg ctgacgtcac atgccggttc cacaatttgg ccataaaaat
    63361 ggatcacgcc cccttgcgcg gtgcttcctg cgatggcaat aaaaggaaaa aacaaactgc
    63421 cgagcataag agagtttaaa aaaggtctga acatttattc tatttcctca atgattagtc
    63481 atccttgagg gttgtgttat ccatactttc aggttggctg attcgcagca gaccattctc
    63541 tccagattca tcttatgctc gatatttcaa caaattaaga cttttctgaa gagggcggcc
    63601 attgttgtga taaatgaagt gactgtccat cagaaacagt aacaactatt ttcacccgat
    63661 cgtccgggga aatatttaaa ctcaacttcg tcaaagcaat gggttgatta gcaggcaatg
    63721 aaagggtctt ttcttgcttc gtctgacttt gccctgaact gccttcgcgc agggacaata
    63781 tttgtactct gcacagacaa gattgagtaa gagtgacttc aggaataatg gtgtacatat
    63841 ccccttgctg ggtcgtatta aaggttatct gactggaaag tgccgcaagg agtaataacg
    63901 cattcatatt cttctcccga aaaaaaacag ggcttgcgcc ctgtttcttt aatacagatg
    63961 atgtattagt actgatgagc ggtcgcgttg ttaccaaagc caacctgagt tacgttgacg
    64021 gtggaattag atgcagtctg gtcaaccgct gcaccgttgc cgccaccgaa ttgtttaact
    64081 gtcatatgag agtctttacc gttccactga tcaagagtgg cgctgttacc aaagccacgt
    64141 tgggtcagat cgattgagct gtcatctgag ccctgaccaa catctgcacc gttaccacca
    64201 ccatgctggg taatagtaag atcagagtta cgagcatcag cttgcagagc aagtgcagag
    64261 ttaccaccac cgtactgata aatattcagc tctgaattcg ggccgctgtt attaccgcca
    64321 ccaccgtggt taccgccacc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
    64381 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
    64441 attgatttaa aagtcgaatg gaaattaacg ttgtgtcacg cgaatagcca tttgcgactg
    64501 tctctgcact acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc
    64561 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
    64621 attggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
    64681 tgcccggttg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
    64741 taactgagca ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga
    64801 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
    64861 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
    64921 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
    64981 acgcaaccta tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
    65041 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
    65101 cacgtaaata cgctgttatc tacgcaaaaa atattttgtt ttcttttaaa tctccgtttt
    65161 ccgctagcaa aaaacaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
    65221 tcttgcg
//
LOCUS       DAZFBR010000012        65108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFBR010000012 DAZFBR010000000
VERSION     DAZFBR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.012"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(113..189)
                     /locus_tag="ACHJZ2_000941"
     tRNA            complement(113..189)
                     /locus_tag="ACHJZ2_000941"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Arg,seq:acg)
     gene            complement(254..330)
                     /locus_tag="ACHJZ2_000942"
     tRNA            complement(254..330)
                     /locus_tag="ACHJZ2_000942"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(294..296),aa:Arg,seq:acg)
     gene            complement(334..426)
                     /locus_tag="ACHJZ2_000943"
     tRNA            complement(334..426)
                     /locus_tag="ACHJZ2_000943"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(390..392),aa:Ser,seq:gct)
     gene            complement(742..927)
                     /gene="csrA"
                     /locus_tag="ACHJZ2_000944"
     CDS             complement(742..927)
                     /gene="csrA"
                     /locus_tag="ACHJZ2_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO4567105.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1162..3792)
                     /gene="alaS"
                     /locus_tag="ACHJZ2_000945"
     CDS             complement(1162..3792)
                     /gene="alaS"
                     /locus_tag="ACHJZ2_000945"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO4567106.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLMNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3920..4420)
                     /gene="recX"
                     /locus_tag="ACHJZ2_000946"
     CDS             complement(3920..4420)
                     /gene="recX"
                     /locus_tag="ACHJZ2_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO4567107.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4488..5549)
                     /gene="recA"
                     /locus_tag="ACHJZ2_000947"
     CDS             complement(4488..5549)
                     /gene="recA"
                     /locus_tag="ACHJZ2_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO4567108.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5629..6126)
                     /gene="pncC"
                     /locus_tag="ACHJZ2_000948"
     CDS             complement(5629..6126)
                     /gene="pncC"
                     /locus_tag="ACHJZ2_000948"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO4567109.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6271..7356)
                     /gene="mltB"
                     /locus_tag="ACHJZ2_000949"
     CDS             complement(6271..7356)
                     /gene="mltB"
                     /locus_tag="ACHJZ2_000949"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO4567110.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7611..8173
                     /gene="srlA"
                     /locus_tag="ACHJZ2_000950"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7611..8173
                     /gene="srlA"
                     /locus_tag="ACHJZ2_000950"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8170..9131
                     /gene="srlE"
                     /locus_tag="ACHJZ2_000951"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             8170..9131
                     /gene="srlE"
                     /locus_tag="ACHJZ2_000951"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            9142..9513
                     /gene="srlB"
                     /locus_tag="ACHJZ2_000952"
                     /gene_synonym="gutB"
     CDS             9142..9513
                     /gene="srlB"
                     /locus_tag="ACHJZ2_000952"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO4567111.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9517..10296
                     /gene="srlD"
                     /locus_tag="ACHJZ2_000953"
     CDS             9517..10296
                     /gene="srlD"
                     /locus_tag="ACHJZ2_000953"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO4567112.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10402..10761
                     /gene="gutM"
                     /locus_tag="ACHJZ2_000954"
     CDS             10402..10761
                     /gene="gutM"
                     /locus_tag="ACHJZ2_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO4567113.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10828..11601
                     /gene="srlR"
                     /locus_tag="ACHJZ2_000955"
                     /gene_synonym="gutR"
     CDS             10828..11601
                     /gene="srlR"
                     /locus_tag="ACHJZ2_000955"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO4567114.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            11594..12559
                     /gene="gutQ"
                     /locus_tag="ACHJZ2_000956"
     CDS             11594..12559
                     /gene="gutQ"
                     /locus_tag="ACHJZ2_000956"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO4567115.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12556..14070)
                     /gene="norR"
                     /locus_tag="ACHJZ2_000957"
     CDS             complement(12556..14070)
                     /gene="norR"
                     /locus_tag="ACHJZ2_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO4567116.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14257..15696
                     /gene="norV"
                     /locus_tag="ACHJZ2_000958"
     CDS             14257..15696
                     /gene="norV"
                     /locus_tag="ACHJZ2_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO4567117.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15693..16826
                     /gene="norW"
                     /locus_tag="ACHJZ2_000959"
     CDS             15693..16826
                     /gene="norW"
                     /locus_tag="ACHJZ2_000959"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO4567118.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDTEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(16954..19206)
                     /gene="hypF"
                     /locus_tag="ACHJZ2_000960"
     CDS             complement(16954..19206)
                     /gene="hypF"
                     /locus_tag="ACHJZ2_000960"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO4567119.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19359..19886)
                     /gene="hydN"
                     /locus_tag="ACHJZ2_000961"
     CDS             complement(19359..19886)
                     /gene="hydN"
                     /locus_tag="ACHJZ2_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO4567120.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20035..21045)
                     /gene="ascG"
                     /locus_tag="ACHJZ2_000962"
     CDS             complement(20035..21045)
                     /gene="ascG"
                     /locus_tag="ACHJZ2_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO4567121.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21305..22762
                     /gene="ascF"
                     /locus_tag="ACHJZ2_000963"
     CDS             21305..22762
                     /gene="ascF"
                     /locus_tag="ACHJZ2_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO4567122.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            22771..24195
                     /gene="ascB"
                     /locus_tag="ACHJZ2_000964"
     CDS             22771..24195
                     /gene="ascB"
                     /locus_tag="ACHJZ2_000964"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO4567123.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24319..24789)
                     /gene="hycI"
                     /locus_tag="ACHJZ2_000965"
     CDS             complement(24319..24789)
                     /gene="hycI"
                     /locus_tag="ACHJZ2_000965"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO4567124.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(24782..25192)
                     /gene="hycH"
                     /locus_tag="ACHJZ2_000966"
     CDS             complement(24782..25192)
                     /gene="hycH"
                     /locus_tag="ACHJZ2_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO4567125.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25189..25956)
                     /gene="hycG"
                     /locus_tag="ACHJZ2_000967"
     CDS             complement(25189..25956)
                     /gene="hycG"
                     /locus_tag="ACHJZ2_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO4567126.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(25956..26498)
                     /gene="hycF"
                     /locus_tag="ACHJZ2_000968"
     CDS             complement(25956..26498)
                     /gene="hycF"
                     /locus_tag="ACHJZ2_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO4567127.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(26508..28217)
                     /gene="hycE"
                     /locus_tag="ACHJZ2_000969"
     CDS             complement(26508..28217)
                     /gene="hycE"
                     /locus_tag="ACHJZ2_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO4567128.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28235..29158)
                     /gene="hycD"
                     /locus_tag="ACHJZ2_000970"
     CDS             complement(28235..29158)
                     /gene="hycD"
                     /locus_tag="ACHJZ2_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO4567129.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29161..30987)
                     /gene="hycC"
                     /locus_tag="ACHJZ2_000971"
     CDS             complement(29161..30987)
                     /gene="hycC"
                     /locus_tag="ACHJZ2_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO4567130.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(30984..31595)
                     /gene="hycB"
                     /locus_tag="ACHJZ2_000972"
     CDS             complement(30984..31595)
                     /gene="hycB"
                     /locus_tag="ACHJZ2_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO4567131.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(31720..32181)
                     /gene="hycA"
                     /locus_tag="ACHJZ2_000973"
     CDS             complement(31720..32181)
                     /gene="hycA"
                     /locus_tag="ACHJZ2_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO4567132.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32393..32743
                     /gene="hypA"
                     /locus_tag="ACHJZ2_000974"
     CDS             32393..32743
                     /gene="hypA"
                     /locus_tag="ACHJZ2_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4567133.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            32747..33619
                     /gene="hypB"
                     /locus_tag="ACHJZ2_000975"
     CDS             32747..33619
                     /gene="hypB"
                     /locus_tag="ACHJZ2_000975"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO4567134.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            33610..33882
                     /gene="hypC"
                     /locus_tag="ACHJZ2_000976"
     CDS             33610..33882
                     /gene="hypC"
                     /locus_tag="ACHJZ2_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO4567135.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            33882..35003
                     /gene="hypD"
                     /locus_tag="ACHJZ2_000977"
     CDS             33882..35003
                     /gene="hypD"
                     /locus_tag="ACHJZ2_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO4567136.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35000..36010
                     /gene="hypE"
                     /locus_tag="ACHJZ2_000978"
     CDS             35000..36010
                     /gene="hypE"
                     /locus_tag="ACHJZ2_000978"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO4567137.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36084..38162
                     /gene="flhA"
                     /locus_tag="ACHJZ2_000979"
     CDS             36084..38162
                     /gene="flhA"
                     /locus_tag="ACHJZ2_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO4567138.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(38199..38552)
                     /gene="ygbA"
                     /locus_tag="ACHJZ2_000980"
     CDS             complement(38199..38552)
                     /gene="ygbA"
                     /locus_tag="ACHJZ2_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO4567139.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(38629..38763)
                     /locus_tag="ACHJZ2_000981"
     CDS             complement(38629..38763)
                     /locus_tag="ACHJZ2_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567140.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            38839..41400
                     /gene="mutS"
                     /locus_tag="ACHJZ2_000982"
     CDS             38839..41400
                     /gene="mutS"
                     /locus_tag="ACHJZ2_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO4567141.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            41506..42162
                     /gene="pphB"
                     /locus_tag="ACHJZ2_000983"
     CDS             41506..42162
                     /gene="pphB"
                     /locus_tag="ACHJZ2_000983"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO4567142.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(42203..42439)
                     /locus_tag="ACHJZ2_000984"
     CDS             complement(42203..42439)
                     /locus_tag="ACHJZ2_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO4567143.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(42450..43877)
                     /locus_tag="ACHJZ2_000985"
     CDS             complement(42450..43877)
                     /locus_tag="ACHJZ2_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO4567144.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(43877..44470)
                     /locus_tag="ACHJZ2_000986"
     CDS             complement(43877..44470)
                     /locus_tag="ACHJZ2_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO4567145.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            44617..45024
                     /locus_tag="ACHJZ2_000987"
     CDS             44617..45024
                     /locus_tag="ACHJZ2_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO4567146.1"
                     /translation="MTLRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(45144..46136)
                     /gene="rpoS"
                     /locus_tag="ACHJZ2_000988"
     CDS             complement(45144..46136)
                     /gene="rpoS"
                     /locus_tag="ACHJZ2_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO4567147.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(46199..47293)
                     /gene="nlpD"
                     /locus_tag="ACHJZ2_000989"
     CDS             complement(46199..47293)
                     /gene="nlpD"
                     /locus_tag="ACHJZ2_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO4567148.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPAHTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(47478..48104)
                     /gene="pcm"
                     /locus_tag="ACHJZ2_000990"
     CDS             complement(47478..48104)
                     /gene="pcm"
                     /locus_tag="ACHJZ2_000990"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO4567149.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(48098..48859)
                     /gene="surE"
                     /locus_tag="ACHJZ2_000991"
     CDS             complement(48098..48859)
                     /gene="surE"
                     /locus_tag="ACHJZ2_000991"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO4567150.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(48840..49889)
                     /gene="truD"
                     /locus_tag="ACHJZ2_000992"
     CDS             complement(48840..49889)
                     /gene="truD"
                     /locus_tag="ACHJZ2_000992"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO4567151.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(49886..50365)
                     /gene="ispF"
                     /locus_tag="ACHJZ2_000993"
     CDS             complement(49886..50365)
                     /gene="ispF"
                     /locus_tag="ACHJZ2_000993"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO4567152.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(50365..51075)
                     /gene="ispD"
                     /locus_tag="ACHJZ2_000994"
     CDS             complement(50365..51075)
                     /gene="ispD"
                     /locus_tag="ACHJZ2_000994"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO4567153.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(51094..51405)
                     /gene="ftsB"
                     /locus_tag="ACHJZ2_000995"
     CDS             complement(51094..51405)
                     /gene="ftsB"
                     /locus_tag="ACHJZ2_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO4567154.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(51599..51922)
                     /gene="ygbE"
                     /locus_tag="ACHJZ2_000996"
     CDS             complement(51599..51922)
                     /gene="ygbE"
                     /locus_tag="ACHJZ2_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO4567155.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(51972..52577)
                     /gene="cysC"
                     /locus_tag="ACHJZ2_000997"
     CDS             complement(51972..52577)
                     /gene="cysC"
                     /locus_tag="ACHJZ2_000997"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO4567156.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(52577..54004)
                     /gene="cysN"
                     /locus_tag="ACHJZ2_000998"
     CDS             complement(52577..54004)
                     /gene="cysN"
                     /locus_tag="ACHJZ2_000998"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO4567157.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            complement(54006..54914)
                     /gene="cysD"
                     /locus_tag="ACHJZ2_000999"
     CDS             complement(54006..54914)
                     /gene="cysD"
                     /locus_tag="ACHJZ2_000999"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO4567158.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            55166..56203
                     /gene="iap"
                     /locus_tag="ACHJZ2_001000"
     CDS             55166..56203
                     /gene="iap"
                     /locus_tag="ACHJZ2_001000"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO4567159.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   56285..56497
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=56346..56375
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacaca"
     gene            complement(56593..56886)
                     /gene="cas2e"
                     /locus_tag="ACHJZ2_001001"
     CDS             complement(56593..56886)
                     /gene="cas2e"
                     /locus_tag="ACHJZ2_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO4567160.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(56883..57806)
                     /gene="cas1e"
                     /locus_tag="ACHJZ2_001002"
     CDS             complement(56883..57806)
                     /gene="cas1e"
                     /locus_tag="ACHJZ2_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO4567161.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(57803..58453)
                     /gene="cas6e"
                     /locus_tag="ACHJZ2_001003"
     CDS             complement(57803..58453)
                     /gene="cas6e"
                     /locus_tag="ACHJZ2_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO4567162.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(58435..59181)
                     /gene="cas5e"
                     /locus_tag="ACHJZ2_001004"
     CDS             complement(58435..59181)
                     /gene="cas5e"
                     /locus_tag="ACHJZ2_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO4567163.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(59192..60247)
                     /gene="cas7e"
                     /locus_tag="ACHJZ2_001005"
     CDS             complement(59192..60247)
                     /gene="cas7e"
                     /locus_tag="ACHJZ2_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO4567164.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(60259..60795)
                     /gene="casB"
                     /locus_tag="ACHJZ2_001006"
                     /gene_synonym="cse2"
     CDS             complement(60259..60795)
                     /gene="casB"
                     /locus_tag="ACHJZ2_001006"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO4567165.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            complement(60792..62354)
                     /gene="casA"
                     /locus_tag="ACHJZ2_001007"
                     /gene_synonym="cse1"
     CDS             complement(60792..62354)
                     /gene="casA"
                     /locus_tag="ACHJZ2_001007"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO4567166.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(62452..>65108)
                     /gene="cas3"
                     /locus_tag="ACHJZ2_001008"
     CDS             complement(62452..>65108)
                     /gene="cas3"
                     /locus_tag="ACHJZ2_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO4567167.1"
                     /translation="TFFDFWGKTRRGEKEGGDGYHLLCWHSLDVAAMGYLMVKRNCFG
                     LADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAPELKIPEGSRKNYEKI
                     SHSTLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITTGHHGRPPDRIDELDN
                     FLPEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSWYISATVVLADWTGSS
                     TRFFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGINTLFPFIEHPTPLQQ
                     KVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAARKAQGLFFGLPTMATANAM
                     YDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCA
                     AWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMS
                     CILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLT
                     HVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQGKCIAWIRNSVDDAI
                     KVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQV
                     LEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAP
                     VWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGED
                     VVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDYLPEKLSTRLAEESVS
                     LWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRQWCIEQRQD
                     PEMANVILVTDDESCGYSAREGLIGKVD"
BASE COUNT        15380 a        17930 c        16723 g        15075 t
ORIGIN      
        1 cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc atcgggaaat
       61 ttattttact gctgatattg ataccgctac taaagctgta tcaagtaaga gatggtgcat
      121 ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg agtactctat ccagctgagc
      181 tacggatgca tcgggaaact tactttactg cagatttttt gataccgcta ctaaagccgt
      241 atcaagtaag agatggtgca tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc
      301 gagtactcta tccagctgag ctacggatgc aaatggcggt gaggcgggga ttcgaacccc
      361 ggatgcagct tttgaccgca tactccctta gcaggggagc gccttcagcc tctcggccac
      421 ctcaccacac gcctcttacg agtgcttcga agaacttgtt tatcgctcat cgtcgctgcg
      481 tggcgcacat attactttct gagacttata agtcaaacaa tttttcccac acttttatcg
      541 tttgcacact tcacgctcaa ttagtctgta aaaacgtcaa aaagagtgtt ttatctacag
      601 aaaaacggag gtctggcaga tagcagtaat gcaaaaaaat ggagacttaa gttgaatgaa
      661 cgggagtaaa gcgaaaagac tatagagtga aggagaaatt ttgagggtgc gtctcaccga
      721 taaagatgag acgcggaaag attagtaact ggactgctgg gatttttcag cctggatacg
      781 ctggtagatc tcttcacggt gaacagaaac ttccttcggg gcatttacgc caatacgtac
      841 ctggttgccc tttaccccta aaactgtcac ggtgacctca tccccaatca tgagggtctc
      901 accaactcga cgagtcagaa tcagcattct ttgctccttg aaagattaaa agagtcgggt
      961 ctctctgtat cccggcatta tccatcatat aacgccaaaa agtaagcaat gacaaacaca
     1021 ttacatgtaa gcagtcatgg cattacattc tgttaaacct aagtttagcc gatatacaca
     1081 acttcaacct gactttatcg ttgtcgatag cgttaatgcg aatgccgtga agcgagtcca
     1141 cggcattgcc tgacgcttat attattgcaa tttcgcgctg acccagcctt tcacactggc
     1201 taacgctgca ggtaaggccg cagcatccgt accaccggct tgcgccatgt caggacgtcc
     1261 accacccttg ccgcccacct gctgagcgac cataccaatc agttcccctg ctttcacacg
     1321 atctgtgacg tccttagata cgcctgcaat cagagaaacc ttaccttcgg ctaccgttgc
     1381 cagcacgata attgtcgacc ccagctgatt ttttaaatcg tcaaccatgg tacgcaacat
     1441 tttcggctca acaccgctaa gctcgctaac caacagctta acaccattaa catcaattgc
     1501 cttactggaa agatttgcgc tctcctgtgc ggcagcttgt tctttaagct gttgtagttc
     1561 tttttctaac tgacgcgtac gttccagtac tgagcgcact ttatccgcca gattattgct
     1621 atcgcctttc agcagatgcg cgacttcgct taagcgatcg ctgtctgcat gaacggtggt
     1681 gatagcgcct tctccggtta ccgcttcgat acgacgaacg cctgcagcag tacccgattc
     1741 agagatgatg cggaacagac caatatcacc agtgcggctg gcgtgagtac cgccacacag
     1801 ctcggtggag aaatcgccca tgctcagcac gcgtacacgc tcatcatact tctcgccgaa
     1861 cagcgccatc gcacctttcg ctttcgccgc ttcgagatcc atgatgttgg tttcgatcgg
     1921 caaattgcga cgaatctgtg cgttcatcag gtcttcgacc gcacgaatct cttccggttt
     1981 catcgcttcg ttgtgtgaga agtcgaagcg cagcaccttg tcgttaacca gtgaaccttt
     2041 ctgcgataca tgagtcccca gaacctggcg cagcgcagcg tgcatcaggt gcgttgcgga
     2101 gtgattcaaa cgaatacggg cgcgacgagc ctcatcaaca tcagcctgca cagcgtcgcc
     2161 cactttcaga gaacccgcag caagtttacc gatgtgacca atcgcctggc cgtatttctg
     2221 cgtatcttcc accacgaagg agaagttagc gcctttcagt tcgcctttat caccaacctg
     2281 accgccggat tccgcataga atggcgtttg atccagcacg accacagctt cctggcctgc
     2341 attgatggca tcaaccgctt taccatcaac aaacagcgcg gtcactttgc cgttcagttc
     2401 cagatggtca tagcctttaa attcagatgc actgtcaaca cggatcattg cgttgtaatc
     2461 ggcaccaaag ccgctggctt cgcgcgcccg acgacgctgc tcttccattg ctgcttcaaa
     2521 accagcttcg tcaactttga tgttgcgctc acgacaaaca tcagccgtca ggtcaaccgg
     2581 gaagccatag gtgtcgtaca gacggaaagc agtttcacca tccagcgtat caccagaaag
     2641 ttttgccagc tcttcatcca gcaacgccag accgcgctcc agagtacgag caaactgctc
     2701 ttcttcagtc ttcagcacct gctcaacctg cgcctgctgg cgtttcaggt cttcacccgc
     2761 agagcccata acgtcgatca gcggaccaac cagtttgtag aagaaggttt ctttcgcgcc
     2821 gagcatatta ccgtgacgca ctgcgcgacg aatgatacga cgcagtacat aaccacggtt
     2881 ttcattggac ggcattacgc catccgcgat caggaacgca caagaacgaa tgtggtcagc
     2941 gattacgcgc agcgatttat tgctcagatc ggttgcgcca gtgactttcg ctaccgcctg
     3001 gatcaacgtg cggaacaggt cgatgtcata gttagagtta acgtgttgca gcaccgcagc
     3061 aatacgctcc agacccatac cggtatctac agacggcttc ggcagcggtt ccatcgtgcc
     3121 atcggcctgg cggttgaact gcatgaagac gatgttccag atctcaatgt agcggtcgcc
     3181 gtcttcttcc ggacttcccg gagggccacc ccaaatgtgg tcgccgtgat cgtagaagat
     3241 ttcggtgcac gggccgcacg gaccagtgtc acccatctgc cagaagttgt cagatgcgta
     3301 tggcgcacct ttgttatcgc cgatgcgaat aatacgttcg cgcgggatcc ctacttcttt
     3361 ttcccagatt tcgtaggctt cgtcgtcgct ttcatagacg gtaacccaca gacgctcttt
     3421 cggcagggca aaccattttt cgctggtcag cagttcccat gcaaactgaa tggcatcgtg
     3481 tttgaaatag tcgccgaagc tgaagttgcc cagcatttcg aagaaggtat ggtgacgcgc
     3541 ggtgtaaccg acgttttcca ggtcgttgtg tttaccaccc gcacgcacgc agcgttggga
     3601 agtggtagcg cgggaataat tacgcttgtc gagcccaagg aacacatcct tgaactggtt
     3661 catcccggcg ttggtaaaca acaaagttgg gtcgttatgg ggtaccaggg agctgctggc
     3721 aactacctga tgtcccttac tatggaaaaa gtcgagaaac gcctgacgga tctcagcggt
     3781 gctcttgctc ataattatcc tgaaatcaag ctaacgaaat atcgccacca gctccagcgt
     3841 gtcttaaccg ccgggctggt aactgaaaag tgggaataag ataagttttc ttgactggga
     3901 agtaaaatac cgtatgcgtt cagtcagcaa aatttcgcca aatatcctgg atatcttcca
     3961 tcagatagcc acgatagagc agaaaacgct ggatcttaac tttttctgaa aagacagttg
     4021 gcaaaggttc accatatttt cgcgtcgcct gatcgcgcgc cagtgcgcac cagtcgatgt
     4081 cacattcacg catcgctttt tctgtcgctt cgcgggaaat acctttctga ttcagttcct
     4141 ggcgaatacg cgcaggtcca taacctttgc ggctacggct ggcgataaag cgcgcaacaa
     4201 atcggctgtc atcgagatag ccatgttcat ggcaccaggc aataacgcgc tcgtaatctt
     4261 ctgccgtagc atcaatctct tctgggccat ttttgcccat aatcggtgcc gcgagtttac
     4321 gtcgcagttc ttgctcactg tgatcgcgca ccgccagaat gcgtaccgca cgatccaaca
     4381 ggcgagcata tgccgggcga cgggacgttg attctgtcat ggcatatcct tacaacttaa
     4441 aaagcaaaag ggccgcagat gcgacccttg tgtatcaaac aagacgatta aaaatcttcg
     4501 ttagtttctg ctacgccttc gctatcatct acagagaaat ccggcgttga gttcgggttg
     4561 ctcagcagca actcacgtac tttcttctcg atctctttcg cggtttccgg gttatctttc
     4621 agccaggcag ttgcattcgc tttaccctga ccgatcttct cacctttgta gctgtaccac
     4681 gcgcctgctt tctcgatcag cttctctttt acgcccaggt caaccagttc gccgtagaag
     4741 ttgatacctt cgccgtagag gatctggaat tcagcctgtt taaacggcgc agcgattttg
     4801 ttcttcacca ctttcacgcg ggtttcgcta cccaccacgt tttcgccctc tttcaccgcg
     4861 ccgatacgac ggatgtcgag acgaacagag gcgtagaatt tcagcgcgtt accaccggta
     4921 gtggtttccg ggttaccgaa catcacacca attttcatac ggatctggtt gatgaagatc
     4981 agcagcgtgt tggactgctt caggttaccc gccagcttac gcatcgcctg gctcatcata
     5041 cgtgccgcaa ggcccatgtg agagtcgccg atttcgcctt cgatttccgc tttcggcgtc
     5101 agtgccgcca cggagtcaac gacgataacg tctactgcgc cagaacgcgc cagggcgtca
     5161 cagatttcca gtgcctgctc gccggtgtcc ggctgggagc acagcaggtt gtcgatatcg
     5221 acgcccagtt tacgtgcgta gattgggtcc agcgcgtgtt cagcatcgat aaacgcacag
     5281 gttttacctt cacgctgcgc tgcggcaatc acctgcaacg tcagcgtggt tttaccggaa
     5341 gattccggtc cgtagatttc gacgatacgg cccatcggca gaccacctgc cccaagcgcg
     5401 atatccagtg aaagcgaacc ggtagagatg gtttccacat ccatggaacg gtcttcaccc
     5461 aggcgcatga tggagccttt accaaattgt ttctcaatct ggcccagtgc tgccgccaac
     5521 gctttctgtt tgttttcgtc gatagccatt tttactcctg tcatgccggg taataccgga
     5581 tagtcaatat gttctgttga agcaattata ctgtatgctc atacagtatc aagtgttttg
     5641 tagaaattgt tgccacaagg tctgcaatgc atacgcagta gcctgacgac gcaccgcatc
     5701 acggtcgccg ctgaagcatt cccgcagggt aatgccttca ccgcgggcag tggcaaaagc
     5761 aaaccagacg gtgccgacag gcttctcttc actgccgcca tccggcccgg cgataccact
     5821 aatagacacg gcataatcag cacgagccgc tttcagtgca cctatcgcca tttccaccac
     5881 gacgggttca ctcaccgcgc catgctgcgc cagcgtctct tcgcgtacgc cgatcatttg
     5941 cgctttggct tcgttactgt aggtgacaaa tccgcgttca aaccaggcgg aactgccggc
     6001 aatatcggta atcactttcg ctacccaacc gccggtacaa gactcagcag ttgttacggt
     6061 tgcgccacgg gctttcagcg cctgcccaac ctgttcgctt aactgcatca gttcactgtc
     6121 agtcatcaca acctctgtca gtcaaaaatt tatgccggac aagatagcac tttcgtcagg
     6181 aagatggtgt tggcgttgca attttacgag gaggattcag aaaaaagctg attagccaga
     6241 gggaagctca cgcccccctc ttgcaaatag ttactgcacg cgcgccagcg ccacggcttg
     6301 tcctaactgc cagaccgcca ttgcgtaatg ggtactgtgg ttataacggg taatggtgta
     6361 gaagttcggc agaccgtacc agtactggta acctgttcca acatccagac gcagcaggct
     6421 ggcttgttga tggttgccca gcggctgctg tggcgttaaa cctgccgcgg caagctgcga
     6481 aatgctgtac ctggttttga agccatttgg caagcctgga gcctgaccgt ttgccattac
     6541 cgcgacctga tcgcctttca cccagccgtg cgctttgaaa taattcgcca cgctaccgat
     6601 cgcatcaacc ggatcccaca ggttgatatg cccgtcgccg ctgaaatcta ccgcatattg
     6661 tttgtaagac gacggcataa actgtccgta gcccatcgcc ccggcaaagg aacctttcag
     6721 attgagcgga tcgtcctgct cgtcgcgcgc catcagcagg aaggtttcca gctcgccaga
     6781 gaaatactcc gcgcggcgtg ggtagttaaa tgacagcgtt gccagcgcat cgaggatgcg
     6841 agttttcccc atcacgcgcc cccagcgggt ttcaacgccg ataatcccga cgataatttc
     6901 cggcggtacg ccatacacct gccacgcgcg attcaacgca tcttcatact gattccagaa
     6961 aaccacaccg ttctgcacat tgtccggcgt aataaatttt ttgcgataac gcagccatgc
     7021 gccgttcgga cctgatggcg gtttcaccga tgtggttggt gcctggttat ccatcagccg
     7081 cagtaccgaa tccagacgtt tcgcctggga gagaatttcc tgcaactgct gacgatcgaa
     7141 accgtgtttg ttcaccattc tgtcgatgaa ctgctgggca ttcgggttat tagcgaaatc
     7201 gccgcccatc tgcatcacat tgtgctgcgg ctcaagcagg aagccgccag acggcgttcc
     7261 ggtggtcgtt tcagtctcag taggttttgg cttgctgcta caggcggcaa gcaacacaaa
     7321 aaggggaagc aatgttacat aacgacgctt gaacatgagg ggtccattta acagattcaa
     7381 ccaggggcaa gtatggtaaa gcatcacgcc ccgcacaagg aagcggtagt cactgcccga
     7441 tacggacttt acataactca actcattctc ctcgctatcc ttttactcaa actttcaaat
     7501 taaaatattt atctttcatt ttgcgatcaa aataacactt taaatctttc aatctgatta
     7561 gattaggttg ccgtttggta ataaaacaat aaatcctgaa ggagagaaca atgatagaaa
     7621 ccattactca tggtgcagag tggtttatcg ggctgttcca aaagggcgga gaggtgttta
     7681 ccgggatggt gaccggcatt cttccgctgt tgattagcct gctggttatc atgaacgcac
     7741 tgattaattt tatcggtcag catcgtattg aacgttttgc tcaacgttgc gccggtaacc
     7801 ctgtttcccg ttacctactg ttaccgtgca ttggcacgtt tgtcttttgc aatccgatga
     7861 ccttaagcct gggtcgcttt atgccggaaa agtacaaacc cagctactac gcggcggcct
     7921 cttatagctg ccactcaatg aatggcctct tcccccatat caaccctggc gaactgtttg
     7981 ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc cactggcggt
     8041 gagctatctg ctggttggtc tggtcaccaa cttcttccgc ggctgggtga ccgatctgac
     8101 caccgctatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc atctggcagg
     8161 agcaacatca tgacgcatat tcggatcgaa aaaggaacgg ggggctgggg cggcccgctt
     8221 gagctggaaa ccacgccggg caaaaaaatc gtctatatca ccccgccggt acccggcctg
     8281 cgattgtcga caaactggca cagcttactg gctggcaggc tattgacgga tttaaagaag
     8341 gtgaacccgc ggaggcggaa attggtgtcg cggtaatcga ctgtggcggc acattacgct
     8401 gcggcattta tccgaaacgg cgtattccca ccattaatat ccactcgacg ggcaagtccg
     8461 gtccactggc gcagtacatt gtggaagata tttatgtctc tggcgtaaaa gaagaaaaca
     8521 tcactgtagt gggtgatgcg acaccacaac cctcttccgt gggccgtgac tatgacacca
     8581 gcaagaaaat caccgaacaa agcgatggtt tactggcgaa ggtgggaatg ggtatgggtt
     8641 ctgccgttgc cgtgttgttt caatctggtc gtgacaccat cgacactgta ttaaaaacca
     8701 ttcttccgtt tatggcattc gtctcggcgc tcattggcat cattatggct tctggccttg
     8761 gtgactggat tgcccacggc cttgctccgc tggcgagcca tccactgggt ctggtcatgc
     8821 tggcgctcat ctgctccttc ccgctgcttt cacctttcct cggcccaggc gcagttatcg
     8881 cacaggttat cggcgtattg attggcgtgc agattggtct cggcaatatt ccgccgcatc
     8941 tggctttacc tgcactgttt gccatcaacg cgcaggcggc ctgcgacttc attccggtcg
     9001 gtttgtcgct ggcggaagcc cgtcaggaca cggttcgcgt cggtgtccct tctgtactgg
     9061 tgagccgctt tttaaccggc gcgccgactg tactgatcgc ctggtttgtc tccggtttta
     9121 tctatcaata gaggctgaaa catgaccgtt atttatcaga ccaccatcac ccgtatcggc
     9181 gcgagtgcca ctgacgccct cagcgaccag atgctcatca cctttcgtga aggcgcgcca
     9241 gcggacctcg aagagtattg cttcattcat tgccacggcg agttgaaagg tgcactccat
     9301 cccggtttgc aatttacgct cgggcagcat cgctatccgg tgaccgctgt tggcagcgtg
     9361 gcggaagaca accttcgcga actgggtcat gtcaccctgc gcttcgatgg tttaaacgaa
     9421 gcggaatttc cgggcactgt ccatgtggca ggccctgtac ccgacgatat cgcgccggga
     9481 tcggttttga agtttgaatc tgttaaggag taaaaaatga atcaggttgc cgttgtcatc
     9541 ggtggtgggc aaaccttagg cgcgttcctg tgccacggtc tggctgccga ggggtatcgc
     9601 gtcgcggttg tcgatattca gagcgacaaa gccgcaaatg tggcacaaga aattaacgcc
     9661 gaatatggtg aaggaacagc gtatggtttt ggtgctgacg ccactagcga gcaaagcgtt
     9721 ctggcgctct ctcgtggggt agatgaaatc tttgttcgtg tggatttact ggtctacagc
     9781 gccggaattg caaaagcagc ctttatcagc gacttccagc tcggcgattt tgaccgttcg
     9841 ctacaggtga atctggtggg ttatttcctg tgtgcgcgtg aattttcgcg tttgatgatc
     9901 cgcgatggga ttcagggacg cattattcag atcaactcga aatccggcaa agtgggcagc
     9961 aaacacaatt ctggttatag cgccgcgaaa tttggtggcg tagggctgac tcaatcactg
    10021 gcgctggatc tggcggagta cggcattacg gtgcattcac tgatgctcgg taacctgctg
    10081 aaatcgccga tgttccagtc gctgttgcca caatatgcca ccaagctggg tatcaaaccg
    10141 gatcaagtcg agcagtatta catcgacaaa gtaccgctca aacgcggctg cgattatcag
    10201 gatgtgctga atatgctgct gttctacgcc agtcctaagg cgtcgtactg taccggacag
    10261 tcgatcaatg tcaccggcgg tcaggtgatg ttctgatcaa cagcggagat ccgttaagga
    10321 tctccgtgag actaatagaa tgcctgatgc gctacgctca tcaggcatac aggacttccg
    10381 ccactacatt aaggaaaagt tatggtatcc gcactcatca ctgtcgccgt tatcgcctgg
    10441 tgtgcgcaac tggccttagg cggctggcaa atttctcgtt ttaaccgtgc cttcgacaca
    10501 ctatgccagc aagggcgggt tggcgtgggc cgttccagcg ggcgctttaa accgcgggtc
    10561 gtggtcgcca tcgccctgga cgatcagcag cgcatcgccg acaccttgtt tatgaaagga
    10621 ctgaccgtct tcgcccgacc gcaaaaaatt cccgcaatta ccggtatgca tgcgggtgat
    10681 ttacagcccg atgtgatctt tccccatgat ccactatcac agaatgctct atcattggcg
    10741 cttaaactga aacgtggata atttcgttgt gaatgttact tgcttgcgaa gttatcattt
    10801 tgaaacctaa atcaggtaat cacgcccatg aaacctcgtc agcgtcaagc cgccattctg
    10861 gagtatctgc aaaagcaggg aaaatgctcg gttgaagaat tggcgcaata ttttgacacc
    10921 acaggcacaa ctattcgcaa agatctggtc attctggaac atgccggaac cgtcattcgt
    10981 acttatggcg gagtggtgtt gaataaagag gaatccgatc cgcctatcga tcataaaaca
    11041 ctcatcaaca cccacaagaa agagctgata gcagaagctg ccgttggttt tattcatgat
    11101 ggcgattcga tcattcttga tgctggcagt accgttttgc agatggtgcc tctgctctcg
    11161 cgctttaata acatcacggt gatgaccaac agcctgcata tcgtcaatgc gttatctgaa
    11221 ctggataatg aacaaactat cctgatgcca ggcggaacgt ttcgtaaaaa atcggcctca
    11281 tttcacgggc agctggcaga gaatgccttc gagcatttca ccttcgacaa attattcatg
    11341 ggtaccgacg gcatcgatct caatgcgggc gtaaccacct tcaacgaggt ctataccgtc
    11401 agtaaggcaa tgtgcaatgc cgcgcgcgaa gtgattttga tggcggactc atcaaagttt
    11461 ggccgtaaaa gccccaacgt agtttgcagt cttgaaagcg tcgataagct gattaccgac
    11521 gcagatatcg atccggcgtt tcgtcaggcg ctggaagaga aagggatcga tgtgatcata
    11581 accggagaga gcaatgagtg aagcactact gaacgcggga cgtcagacgt taatgctgga
    11641 attgcaggaa gcaagccgtt taccggaacg tctgggcgat gattttgttc gcgccgccaa
    11701 tatcatcctg cactgcgaag gcaaagtggt ggtttcggga attggcaaat cgggccacat
    11761 tggtaagaaa atcgccgcaa cgcttgccag taccggcaca ccggcttttt ttgtccatcc
    11821 ggcagaagcg ctgcatggcg atctggggat gatcgaaagc cgcgatgtga tgctgtttat
    11881 ctcttactcc ggtggggcga aggaactgga tctgattatt ccgcgtctgg aagacaaatc
    11941 tatcgcgctg ctggcgatga ccggcaaacc gacgtcaccg ctgggcctgg cggcgaaagc
    12001 ggtgctggat atctccgtag aacgcgaagc ctgcccgatg caccttgcgc cgacctccag
    12061 caccgtcaat accctgatga tgggtgacgc gctggcgatg gcggtcatgc aggcgcgcgg
    12121 atttaatgaa gaagattttg cccgctccca ccctgctgga gcactgggcg ctcgcttgct
    12181 gaataaagtg catcatctga tgcgccgtga cgatgccatc ccacaggtgg cgttaaccgc
    12241 cagcgtgatg gatgctatgc tggaactcag ccgcaccggt ctggggctgg tggcggtatg
    12301 tgacgctcaa caacaggtac aaggcgtctt taccgacggc gatttacgtc gctggctggt
    12361 tggcggcggc gcactcacca cgccagtcaa tgaagcgatg acggtcggcg gcaccacgtt
    12421 gcaatcgcaa agtcgcgcca tcgacgccaa agagatcctg atgaagcgca aaatcactgc
    12481 cgcaccggtg gtggatgaaa acggcaaact caccggcgca ataaacctgc aggatttcta
    12541 tcaggccggg attatttaat ccttcaatcc cagacgtttc gccagccgat gcaggttggc
    12601 gacgtcggtt tccagcatcc gcgcgcaggc agcccagttg tgatgatttt gtgccagtgc
    12661 ctgacgaata gtttcacgct ggaacgcttc tgtcgcttca cgcaggtttt gcttaacaac
    12721 gggcaccgcc gccacttctg gcggcggcaa cgtcacctca ggaaaagcaa aatgttgcgc
    12781 ctcaagaatc acttcatcgc cgctgcgggt ggctctcgcc agcactaccg cccgatgaat
    12841 agcatgttcc agttcgcgca cgtttcccgg aaaactgtag tgttgcagta aatttcgcgc
    12901 cccggcactt aataccacgc gggagagccc ctgccgcaaa cgacactgct cgcagaaata
    12961 ccccgccagc agaatgacat catcgccccg ctcacgcagc ggcggcaccg aaagtggaaa
    13021 cacactcagg cgatgaaaca aatcggcgcg gaatcgccct gccagcacct cttcgcgtaa
    13081 gtcgcggtta gtcgccgcca gcacgcgcac atcgacccgc aaactacggt catcgccaac
    13141 acgctgaata tcgccatact gcaacaccct cagcagcttg gcctgcaatg ccaacgacaa
    13201 ctcgccgatc tcatcgagaa acagcgtgcc gttatccgcc atttcaaact tcccactgcg
    13261 gttactgata gcgccagtaa acgctccttt cacatgcccg aacaactcac tttccgccac
    13321 actttccggc agtgcggcac agttgagata gaccagcgga ttcaccgccc gtggcgaggc
    13381 ctcatgaatc gctttcgcca ccagctcctt accggttccc gtctcaccgc tgatcaggac
    13441 gttgagatcg gacgccgcca caatctcaat ctcttttttc agttgcgtca tgcctggcga
    13501 cagaccgatc atctgcgtct gtttcaccgc ttcaaatgga gcggcatcgc ccggcagcat
    13561 attctggctt tccagttgtt caatcagcaa cgcattgctt aacgctcccg ccgccagcgc
    13621 ggcaatcagg cgtaactctt cgtcgctgaa aacatcgaac tgatcgggct gcatcccgtc
    13681 gagcgtcaat gcgccgatca ggttttgccc ggcaaacagc ggcagaccaa cgcaggcgtg
    13741 aaccttcaga ctctcctgcc ctggaatcaa accgtcatag ggatcgggca attcgctgtc
    13801 tgcgggaaag cgcaccacat cacccgcgcg ggcaatcgct tccagccgtg gatgaccttc
    13861 cagcgcaaag cgtctaccga gtacatcctt cgccagcccg tcgatggcaa gcggaataaa
    13921 ctgccgcgag tcgtaacgta gcaacgcaga cgcatcgcac tccagcacct gacgcagcgt
    13981 ggtgatcagg cgctgaaaac gatcctgatg accaatccca cgctgcaatt cgatggcgat
    14041 attcgccagc acatcaacgg aaaaactcat ctttgcctca ctgtcaattt gactatagat
    14101 attgtcatat cgaccattgg attgatagtc attttgacta ctcattaatg agcataattt
    14161 tatttataga gtaaaaacaa tcagataaaa aactggcacg caatctgcaa ttagcaagac
    14221 atctttttag aacacgctga ataaattgag gttgctatgt ctattgtggt gaaaaataac
    14281 attcattggg ttggtcaacg tgactgggaa gtacgagatt ttcacggcac ggaatataaa
    14341 acgctgcgcg gcagcagcta caacagctac ctcatccgcg aagaaaaaaa cgtgctgatc
    14401 gacaccgtcg accataaatt cagccgcgaa tttgtgcaga acctgcgtaa tgaaatcgat
    14461 ctggcggata tcgattacat cgtgattaac catgcagaag aggaccacgc cggggcgctg
    14521 accgaactga tggcacaaat tcccgatacg ccgatctact gtacagccaa cgctatcgac
    14581 tcgataaatg gtcatcacca tcatccggag tggaatttta atgtggtgaa aactggcgac
    14641 acgctggata tcggcaacgg caaacagctc atttttgtcg aaacaccaat gctgcactgg
    14701 ccggacagca tgatgactta cctgacaggc gacgcggtgc tgttcagtaa cgatgctttc
    14761 ggtcagcact actgcgacga gcatctgttc aacgatgaag tggatcagac ggagcttttt
    14821 gagcagtgcc agcgttacta cgccaatatc cttacgccgt tcagccgtct ggtaacgccg
    14881 aaaattaccg agatcctggg ctttaactta ccagtcgata tgatagcgac ctctcacggc
    14941 gtggtatggc gcgataaccc gacgcaaatt gtcgagctgt acctgaaatg ggcggctgat
    15001 tatcaggaag acagaatcac cattttctac gacaccatgt cgaataacac ccgcatgatg
    15061 gctgacgcta tcgcccaggg gattgcggaa accgacccac gcgtggcggt gaaaattttc
    15121 aacgtcgccc gaagcgataa aaacgaaatc ctgaccaatg tcttccgctc aaaaggcgtg
    15181 ctggtcggca cttcgacgat gaataacgtg atgatgccga aaatcgccgg gctggtggag
    15241 gagatgaccg gattacgctt ccgtaacaaa cgcgccagtg ctttcggctc tcacggctgg
    15301 agcggcggtg cggtggatcg tctttccacg cgcctgcagg atgcgggttt cgaaatgtcg
    15361 cttagcctga aagcgaaatg gcgaccagac caggacgctc tggagttatg ccgtgaacac
    15421 ggtcgcgaaa tcgcccgtca gtgggcgctc gcgccgctgc cgcagagcac ggtgaatacg
    15481 gtagttaaag aagaaacctc tgccaccacg acggctgacc tcggcccacg gatgcagtgc
    15541 agcgtctgcc agtggattta cgatccggca aaaggcgagc caatgcagga cgttgcgcca
    15601 ggaacgccgt ggagtgaagt cccggataac ttcctgtgcc cggaatgctc cctcggcaaa
    15661 gacgtctttg acgaactggc atcggaggca aaatgagcaa cggcattgtg atcattggtt
    15721 cgggcttcgc cgcccgccaa ctggtgaaaa atattcgcaa acaggacgcc agtattccat
    15781 taaccctgat tgccgccgac agtatggatg agtacaacaa acctgacctc agccatgtta
    15841 tcagtcaggg gcaacgtgcc gacgacctta cccgccagac ggcgggtgaa tttgccgagc
    15901 agtttaatct gctcctgttt ccgcaaacct gggtgacgga tatcgatacc gaagcccgtg
    15961 tggtgaaaag ccagaataat cagtggcaat acgacaagct ggtgctggca accggtgcca
    16021 gcgcctttgt cccgccagtg cctgggcgtg agttaatgct gacgttaaat agtcagcaag
    16081 agtatcgcgc ctgtgaaacg caactgcggg atgcccgacg cgtgttgatt gttggcggtg
    16141 gtttgattgg tagcgaactg gcgatggatt tttgtcgtgc aggcaaaatg gttacgctaa
    16201 tcgacaacgc tgccagtatt ctggcgtcgt taatgccacc ggaagtaagc agtcgcttgc
    16261 agcatcggtt gacggagatg ggcgttcatc tgctgttgaa atctcagttg caggggctgg
    16321 aaaaaacgga ttctggcatt ctggcaacgc tggaccacca gcgcagcatc gaagtggatg
    16381 cggtaattgc cgccaccgga ctgcgcccgg aaaccgccct ggcacgacgc gccgggctga
    16441 cgattaatcg cggcgtttgc gtcgatagtt atctgcaaac cagtaatgcc gatatttacg
    16501 cgctgggcga ttgcgcggaa attaacggtc aggtattgcc gttcctgcag ccgattcaac
    16561 ttagcgcgat ggtgctggca aaaaatcttc tcggcaataa cacgccgctg aaactaccgg
    16621 cgatgctggt gaaaatcaaa acgccggaat taccgctgca tctggcaggc gaaacccagc
    16681 gtcaggattt acgctggcag attaataccg aacgccaggg aatggtggcg cgcggtgttg
    16741 acgatgcaga ccaacttcgc gcctttgtgg tcagtgagga tcggatgaaa gaggcatttg
    16801 gattgttgaa aacgttgccg gtgtaggtgg gctactgtgc ctaaaatgtc ggatgcgacg
    16861 ctggcgcgtc ttatccgacc tacggggacg catgtgtagg ccggataagg cgtttacgcc
    16921 gcatccggca atggtgtcca aatgcaacac gttttatccg ttctggactt cacccgctaa
    16981 ccaacgcgcc gcagcaataa ccccctgccc cagagacaaa ccgccatcac ccgccggtaa
    17041 actctgtgga aacagcaatg tgaaatcagc gagataatgc gccagacgtg cacgcagcaa
    17101 acggttatga ataaccccgc cgctaaatac cagcgtagtg ataccacgca tcgtggcctg
    17161 ctcacgcatc aacgcggcaa aaccctgcgc cagcgcatca tgaaacgccc acgcgcgttg
    17221 attaaccggt gcctgccagt tcagccactg ctgccagaaa gtggcgagat ccagttgatt
    17281 gtccacccgc ggcatcgtca ccggatgcgt cactccgtcg catgaggctg ctagcgcctc
    17341 cagagcacaa gccgcttcac cttcataact taacgtggct ggcgcacagc ccagtgccgc
    17401 cgccacagca tcgaacaaac gcccacacga tgacgccagc ggcgcgttaa ttccacgctc
    17461 aatggcccgc gccagtacgc tccagttttg ctgttgcaca cttgccgttt cagggtaatt
    17521 ctgccactcc ggcacaaagc gcaggcactg cgccagcagg ttacgccacg gctgcttcgc
    17581 cgccaaatcg ccacccggaa gcgccactgc aggcaagccg cccaggtgct cacattcgcg
    17641 atagttcacc cgcaggcact cgccgcccca caaagcgccg ttctccccca taccgatacc
    17701 gtcgagcgtc aaagcaatga catcaccgcc atccagcggc cactgatgct ctgccagaca
    17761 cgccgctgcg tgggcatgat gatgcagcac cgtttgcgtc ggcagattca tttcgctcgc
    17821 ccactggcag gagacatagc ccggatgtgc gtcatgcaca acgtattgcg gagtgaaatt
    17881 gtagatgttt tgcatcaggc gtaacgcttc gcgccactgc gtctggatgc catcgtcact
    17941 taaatcgccc agatgctggc tcaacaccac ttgttcaccg cgcaccaggc agaaggtatt
    18001 tttcagatcc gcgccgagac acagtacagg cggaacattt ttaaagcccg gaggcaaagc
    18061 cagcgcatcc ggcacatacc cccgcgaacg gcgcagcatt tcgccgcttt cgcgcaccac
    18121 cgaatcatcc atccgctgca caatgtcgcg gttgtgtatt aagaatccgt cggcaatgcc
    18181 ctgcaaatcc tccagcgcct gttcgttgct gatagccggt ggtttaccgc tcaggttgcc
    18241 agaggtcatc accagcgggc attgtagttc ctgtaacagc aaatgctgaa gcggattcgc
    18301 aggcaacatt accccgactt cgttaaggcc aggggcgata tcatcacaaa gctcaggaac
    18361 gtatttttta tccaccagca caatcggcgc ggcgggcgtg gtaagcaact ggcgcgcagc
    18421 gtctggtaaa ccgtcagcca ctggcaacat gactgccagc ggtttcgccg ggcgatgttt
    18481 gcgcgcccga agtgtcgcca ccgcgttact gttacgtgca tcgcaggcaa gatgaaatcc
    18541 gccaatccct ttgatggcga caatgttgcc catttttaac tgtgcgatag ccgcctgtaa
    18601 tgccgcctct tgttctgcat gttcaccatg acttacccat tcaagatacg ggccacactc
    18661 cgggcaggcc accggctggg cgtggaagcg acgatcgagc gggtcgcggt actctttatc
    18721 acaggctgga cacagcggaa acgccgccat cacggtaaac gggcgatcgt aaggcatggc
    18781 gcgaataatg gtgaagcgcg ggccgcagtg ggtacagttg ataaatggat aacgataacg
    18841 ccgttcgcct ggggtattca tttcggcaag gcaagcaggg caagtggcgg catcgggaac
    18901 aatttgcgta ttcatggcgc cgcccgcgct ctggcggatg gtgaactcag tgggcagttg
    18961 tgaccagata aacggttcac gctcaacgct atcaatacgc gccagcggcg ggcagtgctg
    19021 atgcaattga acaagaaacg tttccgggtc ttccagcagc cggacttcta cgccatcgcc
    19081 gtcattacag acatcgccgt gaagatttaa ttgctgtgcc agctgccaga caaacggacg
    19141 aaaaccgacg ccctgcactt tgcctcgaat acgcagttgg acaccgcaag atgtgttttt
    19201 tgccattgag ttattcccgc catcatgaat tgcgtaaccc gtccagccgg acacgacagc
    19261 gtcgcatccg gcagtcacag gtcggcgata ccgcccgctc cgtattctac gaatatttcc
    19321 gggaattcct ttgatgccag aacagttctg taagattttt agaacatcag cgccgtacgg
    19381 cggcgttttt ctgcgctcag ttgttcaagt ttattgcgat cgacacaaat tagcgcatgg
    19441 gtcgggcaag ccgccataca cgccgggccg tcttcacgat ggttgcacag gtcgcattta
    19501 ttggcttcgg ctttgtcagc ccgtacattc agccctgcgc cgctgttgcg aatcaccgga
    19561 cgtaccacca cttccatcgc gccatacggg caagccacaa cgcaggtttt gcaaccaatg
    19621 caacgttcct gcatcacatg aacaaaccct ttatcacggc tgatagcacc attcgggcag
    19681 acgttagcgc acggtgcatc ttcacactga cggcaaactg tcgccgtgga aatgttcaca
    19741 cctttaatga catggatacg cggtaaaaat gtttccgggg tcagcgatgc acagtcctga
    19801 ttttcctgat gagaaaccac gcacgccact tcacaggtac ggcaaccaat acatttactc
    19861 gcgtcagcaa tgatgaaacg gttcatcaaa ttctccagca atgacagtta atgcgccgat
    19921 acattcacaa atcatgccag tttttaattt gctgttattt aaggaaatta atttctgtaa
    19981 tgcaggaaaa aagatgtcat cgacactagt gacgatgaca tgtgatgaca atgtttatcg
    20041 cgaaagagca atgagtgagt cgcggcggat cagttttccg ctgaaggttt tcggcggtga
    20101 gaaatccccg ccatcgagca taaaaatcag ccgtccaata atttcctgaa tcatctcagt
    20161 caccggaatt tttacgctgg agagcgccgg aacgatatag ggggcaattg cgatatcatc
    20221 gaatccgata actgacacct gctctggcac cgctacgccg cgcttgtgta acgctttcat
    20281 cgcacctatc gccatatcgt cgttactggc aactaacgcg ctaaatttag ccccacgttc
    20341 gagcaacgtt tctacccctt cggccccgct ggcgggcgtc catttaccgt tagcgataag
    20401 tttttcattg agcgcaatac catgctgcgc cagcgcgtct ttatatccgg caagacgttc
    20461 aacgctggtg ggggaatcca tcgagccggt aagaaaagca atctcctgat gcccggcgtt
    20521 tatcaactct gccacggcgt taaagctggt ctgtttatga tcgcaccaga cgctatggct
    20581 gctatttttg cgcaggcggc gattaagcac cattatcggc tgactgtgcg cgtcaatgat
    20641 gtcatcgatc tcatccacac ttaaaaaacg cgggtaaatc ataatggcgt cgcagcgcaa
    20701 atccagcaga tactgaatcg cctggcgctc ttcttcggcg ctgtgtttac catctgccaa
    20761 tagcaactgc cgccctttct cttccgccat tcgcgcggca tgaaacagta attcactaaa
    20821 ataaatgccg tggtaaagcg tgttagtcac taccagcccc agcgtctggg tacttttcgc
    20881 cgacagattg cgcgccagca agtttggacg gtaaccgctc tcttctaccg cctgaaacac
    20941 gcgatcttta gtctcctggc tgacgtagcc attacctgaa agcacgcggg aaacggtcgc
    21001 ttttgaaacc ccggcgcgct tcgccacttc cagcatcgtc gtcatcattt tcatcccttt
    21061 acacgcaatc aacgcagtgt actgcaccgt ttaccgattg tccttgcgca atcggcggga
    21121 aaaatattca ggtgaccggt ttcacaaata taaaaaatga acaattaact ctcttgctta
    21181 tttggttaca actattcatg attttgtgaa accggtttct taattccgtt tcagcatcgg
    21241 catttttccg tcacgtcgac tgataacaac tacatcttcc ctactgataa caggataaaa
    21301 tccgatggcc aaaaattatg cggcgctggc acgctcggtg atagcggcac tgggcggcgt
    21361 tgataacatc tcggcggtca cgcactgtat gacgcgtttg cgctttgtta tcaaagatga
    21421 tgcgcttatc gacagcccga cgttaaaaac catccccggc gtgctcggcg tggtacgtag
    21481 tgacaaccag tgtcaggtga ttattggcaa taccgtttca caagcctttc aggaagtcgt
    21541 cagcctgctg ccgggagata tgcagcccgc cctgcccgtg ggtaaaccca aactcacgct
    21601 gcgtcgcatt ggtgcgggga tcctcgatgc gctgatcggc accatgtcac cgctgatccc
    21661 ggcgattatc ggcggatcga tggtcaaact gctggcgatg atcctcgaga tgagcggcgt
    21721 gctgacaaaa ggatcgccga ccttaaccat tctgaatgtg attggtgacg gtgctttctt
    21781 cttcctgccg ctgatggtcg cggcttcggc cgccatcaaa tttaaaacca atatgtcgct
    21841 ggcgattgcc atcgcgggtg tgctggtaca tccgagtttt attgaactga tggcgaaagc
    21901 ggcccagggt gaacatgtcg aatttgccct gattccagtc accgcggtga aatacaccta
    21961 cacagtgatc ccggcgctgg tgatgacctg gtgcctgtca tatatcgaac gctgggtgga
    22021 tcgcattaca ccggcggtga ccaaaaactt cctcaagcca atgctgattg tgttgattgc
    22081 cgcaccgctg gcaatcctgc tgattggccc gattggtatc tggatcggta gcgccatttc
    22141 ggcgctggtt tacaccattc atggttatct gggctggctt tcagtcgcca ttatgggcgc
    22201 gctgtggcct ctgctggtaa tgaccgggat gcaccgcgtc tttacgctaa ccatcattca
    22261 gaccattgcc gaaaccggca aagaagggat ggtcatgccg tcagagatcg gcgctaacct
    22321 gtcgctgggc ggttcatcac tggcggtggc gtggaaaacg aaaaacccgg aactgcgcca
    22381 gactgcgctg gctgcggcgg catcagccat tatggcgggg atttccgaac cggcgttata
    22441 cggcgtggcg atccgcctga aacgtccgct tatcgccagt cttatcagcg gttttatttg
    22501 cggcgcggtt gccggtatgg cggggcttgc cagccactca atggcagcgc cggggctatt
    22561 taccagcgtg cagttcttcg atccggcgaa tccaatgagc atcgtctggg tgttcgcggt
    22621 catggcgctg gcggtggtgc tgtcgtttat cctcacactg ttgctcggct ttgaggatat
    22681 tcctgttgag gaagcggctg ccgaggcgcg aaagcatcag agcgcacaac cgaccgtcgc
    22741 caaagaagta agtcttaatt gaggatgaaa atgtcagtat ttccagaagg ttttttatgg
    22801 ggcggcgcgc ttgccgccaa ccagtctgaa ggtgcgttcc gtgaaggtgg caaaggactg
    22861 acaacggtcg atatgatccc acacggcgag catcgaatgg cggtgaaact ggggctggaa
    22921 aaacgttttc agttgcgcga tgacgagttt tatcccagcc atgaggcgac gggtttttat
    22981 catcgttata aagaagatat cgccctgatg gcagagatgg gattcaaagt gttccgtacc
    23041 tcaattgcct ggagccgccc cttcccgcag ggtgatgaac tgacgcccaa ccagcagggc
    23101 attgcttttt accgttctgt ctttgaagag tgtaaaaagt acggtatcga accgctggtc
    23161 acattgtgcc acttcgatgt gccgatgcag ctggtcaccg aatatggctc ctggcgtaac
    23221 cgcaagctgg tggagttttt cagccgctac gccaggacct gctttgaagc atttgatggt
    23281 ctggtgaaat actggctaac cttcaatgaa atcaacatta tgttgcatag cccgttctcc
    23341 ggcgcggggc tggtgtttga agaaggtgaa aatcaggatc aggtgaaata tcaggccgcg
    23401 catcaccagc tggttgccag tgcgctagcc accaaaatcg cccatgaggt taacccgcaa
    23461 aatcaggtgg gctgtatgct ggcgggcggt aacttctacc cttacagttg caagccggaa
    23521 gatgtctggg cggcgctgga gaaagatcgg gaaaacctgt tttttatcga tgtgcaggcg
    23581 cggggcgcgt atccggctta ctctgctcgc gtattccgcg aaaaaggggt aaccatcgac
    23641 aaagcaccgg gcgatgatga gatcctgaaa aacaccgtcg attttgtctc tttcagctat
    23701 tacgcctcgc gctgcgcctc ggcggagatg aacaccaaca atagcagtgc ggcgaacgta
    23761 gtgaaatcgc tgcgtaaccc gtatctacag gtgagcgact ggggctgggg aattgatcca
    23821 ctcggtctgc gtatcaccat gaacatgatg tacgaccgct atcagaagcc gctgtttctg
    23881 gtggaaaacg gcctcggcgc aaaagatgaa cttgctgcca atggcgagat taacgatgac
    23941 tatcgcatca gctatttacg cgaacatatc cgcgcaatgg gcgaagcgat tgcagatggc
    24001 attccgctga tgggctacac cacatggggc tgtattgatt tagtttccgc ctctacgggt
    24061 gaaatgagca aacgctacgg ttttgtcttt gttgaccgtg acgacgcagg caacggcacg
    24121 ctgacgcgca cacgtaagaa atcgttctgg tggtataaaa aagtgattgc cagtaatggg
    24181 gaagatttag agtaggtaac ctactgtcgc actaaccagg aaggcccgat aagcgttgcg
    24241 catcgggcaa tctggggttt gctatcagtt ctgattccct catcaagaac atccctgtcc
    24301 tgattactta ttgaaaaact actcttcttc caccgctaac tgcgcgaagc cgccgttccc
    24361 ttcccagcct tccagtcgtt gataaacggt ttctaccgca tctttaatcg gctgggtcat
    24421 cgggtagtaa aagccgacga tatccggctg gatgccgagg aaaatcactt cgccaatatc
    24481 ttctttcaac tgatcgataa ggtagttcaa cggcatgtta tgggtagtca tcataaacat
    24541 ctcggcgata tcatccgggt cgatgatgcg gatttcgccg gggtttagcc ccatatccgt
    24601 ggcgtcgaca atcagcagcc gtgtcgggcg cagttcgcgg atagcgacaa tgtcgttttc
    24661 cggtgcgcta ccgccgtcaa tcaccaccca gttacctttc ggcgcggcgg cgcacttttc
    24721 cgccagcagc ggacctgcgc catcatcacc catcatgcta ttgccaacac agagtaaaac
    24781 gtcagtcacg cagtctcctc accatcaaat agatggcgtt ttcctgatga atatcatgca
    24841 gcatacttaa cagcattgta ctccattcct gctgctgcga agtttgcgct gcccgggctt
    24901 tatcgaatgc attggccagc atcggcacat ggttaatgtc gatgacgatc tcaccatatt
    24961 tcggcacacc ttccattttg cggcgggctt cgctgcccgc ctccagagtg gcaatccatg
    25021 ccagatattc atcccacggg caggtgagcg ccgcttccag gcaatcgata accccaaggt
    25081 ggtgaccaat cgccaggctg taatagacca cctgctgcgc ctcggcgggc gtggcatcgt
    25141 tctcatcaat aaatttacgg ctcagttgac tgaacaccac cttttcactc atcggatacg
    25201 cgcctcttca acaacatgat tcagatggct gacaatctcg ttcagacgcg gatcgttttc
    25261 cgcttccagc cagcgtgcaa cctgttcttc gccctgccct aactgtgtaa ggtaatcatc
    25321 ggcaatctga cgaccgtaac gataacccgc cagtcgacgt gcttcgcgat ccactttcac
    25381 acgcagcggc tgcaccatat cgccatgcag gatctccgcc ggttgttcat cctgctcacc
    25441 cggcccacgg gcgtgaattt tctgctccag caggccgagc gccattgcaa agccgtacag
    25501 cgtggcggct ggcgttggcg ggcagccagg gatataaaca tccaccggga cgattttatc
    25561 cgtaccgccc cacacgcagt agagatcgtg gaagatcccg ccgctgttac cgcaggcacc
    25621 gtaggagata caaattttcg ggtccggcgc ggactgccac gcacgcagcg caggggatcg
    25681 cattgcacgg gtgaccgcgc cggtaaacag taaaatatcc gcatgacgcg gtgaaggaac
    25741 gactttaatg ccgaagcgtt ctgcatcgaa cagcggcgac aaagtggcga aaatttcgat
    25801 ttcgcagccg ttgcagccgc cgcagtccac gcggtaaacg taggcagaac gtttgatttt
    25861 tttcagtaac gacgccttca tgctggcgat ggattcatcc accgtcatcg ggaccggaat
    25921 gccgttggcg tcacgggggc ctaataaatt gctcatcaga tggcctcttt catatggcga
    25981 gtcagttcaa tacggtcgga cggcaccagg catttctggc gcttacattc cgggcaagtc
    26041 tcaaagcttt cgcggtggtt ttccgcgcgg ctgtcgccat tgtgcttaag cagcgcaatg
    26101 gcgtagtcga tctctttctg gacggcgaaa ggacgattgc agacgcggca gttgcacagc
    26161 gcgaagcggg actgttgcag gaagtcttct ttcttccaca ccgccagttc gtactcttgc
    26221 gacagtttga ttgccgccgt cgggcagact tcttcgcagc gtccgcagaa gatgcagcgc
    26281 ccgagattga actcccacgc cagctcaccg gtggcgaggt cagtttcaac cgttaaggcg
    26341 tttgacgggc aggcattgac gcaggccgcg cagccgatgc actgctgcgg gttctgctct
    26401 ggcttaccac ggaagttttt atcaaccgca atcggttcca gcggataaga cgaggtcgcc
    26461 gtgccggttt tgatgacttt tttgataaag gtaaacatgg cgattcctta tttcagcggc
    26521 gagtttttac gctcaatgct gtaacgctcg agttctttgt acggcaccac tttgctcttt
    26581 ttcttacgca catcgaccac ggtcatgcgg tcggtacagg agtagcaagg gtccagacta
    26641 ccgataatca gcggcgcatc agaaacggtg ttgccgcgca gcatgtagcg cagggtcggc
    26701 cagttcgcgt aggtcgcggc acggcagcgc cagcggtaca gcttctggtt gtcgccggtc
    26761 atgctccagt ggatatcatc gccgcgcggc gcttcggcaa agcccagcgc aaaacggtgc
    26821 ggaatgtagg taaagccttc caccatcagc gggccgcccg gcaggttatc cagaccgtag
    26881 tcgatcatgt tcagcgcggt atagacttcg ttaatacgca ctttcagacg cgaaataacg
    26941 tcgcagccct gctcgctgtg gacttccatt ggcagcaggc cataaccaac aaacgggtga
    27001 tcggcgcggg tatcgcgggc gtgaccgctg gcacggacca tcgggccgac gttactgaag
    27061 tcgcgagcga tttccgggtc cagacgacca atgccgacag tgcgctgttc catgttcggc
    27121 gtactcagca gcacatccac cagctcctgc acttcacgac gcatctgttg cgccagttga
    27181 cgggtctgga tcatgtcatc tttcagcaga tcgcgacgaa tcccgccgat caggttcagg
    27241 ccgtaggttt tacgcgcacc ggtaaggatc tctgccattt tcatggaggt ttcacgcacg
    27301 cggaagaact gcataaagcc ggagtcaaag ccggtaaagt gacaggcgag gccgaggttg
    27361 agcagatgcg agtgcagacg ttctacctcc agcagaatgg cgcggatcat ctgcgcacgt
    27421 tctggcacct gaatacccat cgcgttttcc accgacgtgg tgtaggcggt gctgtgagca
    27481 aaaccgcaga tcccgcacac acggtcagag aggaaggtca cttcgttata acccatgcgg
    27541 gtttctgcca gtttttccat gccgcgatgg acatagaaca ggcggtagtc ggcgtcgata
    27601 atgttttcgc catcgacgaa cagacggaag tggcccggtt cgtcagaagt gacgtgcagc
    27661 ggaccaatcg gcacgacgtt gtttttcttg tcgcccagtt cgttgatgaa ctcgtaggtt
    27721 tcagcatcgg tggtcggtgc cggacgctga cgataatcca tgctgtcttt acgcagcgga
    27781 taaagttcat ccggccagtc atccggcagc accagacgac gttcatccgg caaaccaacc
    27841 ggaatcaaac cgtacatatc acgtacttca cgctcgcccc acaccgcagc cggaacgcgc
    27901 ggcgtcacgg aaggatactc cggtttgttg gcatcaactt cgacgcgaac cgttacccag
    27961 cacttagtcc ccttctccat cgacagcacg tagtaaacgg cgtaatgacc attcagtttg
    28021 cgttcgtcgt taccaaaaag caccgacagc cagcccccct gcttgtagta gagaaactcc
    28081 accacttccg gcagatagtt cacctttacg gtgacagtca gctgatcttt ggtctgccag
    28141 gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct
    28201 aatttttctt cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg
    28261 ctaaaaatgc aaagccaaac ccagcccagg taatgcgcgg agtaatatca agacgcagac
    28321 gggccatgct gttttcgaac agcgcgataa ccaggacgcc gaccaccagt ttgacgatgg
    28381 caatcaccaa cgccagcagc agcccaccga cggtgaaggt ttccatttgt ccccacggaa
    28441 taaacacccc gacgaacatc tgcagcacca ccaactgttt caggctgata ccccatttca
    28501 tgacgccaaa gccgctgccg ctgtactcag agagcggacc ttcctgcaac tcctgctcgg
    28561 cttccgccag gtcgaacggc agtttgccca tttcgataaa agtggcgaac gcacaggcac
    28621 aaagcgccag caccagcggg atgctctggc tcagcggcca gtgataaacg gtgtcggtga
    28681 tgttgctgat gttggtggaa ctggcaacct gtgcggcaac ccacagacca agcagcagca
    28741 tcggttcgac cagcacgcca agcatcgctt cacggctcgc gccgatagcg gtaaacggac
    28801 taccggtatc cagaccagaa atggcaaaga agaaacgcgc gatggcaaag agatacagta
    28861 aggtgatcaa atcacccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag
    28921 tagcgatagt cagcatgacg cccaccatca catacggcgt caggcggaac acccagccgg
    28981 aggcatccgg gccaacgctc tggcgaccca gcagtttgat aatgtcgcga tactcctgca
    29041 acacgcccgg cccgcgacgg ttatgcaagc gggcgcgcgc cacgcgggta ataccggaga
    29101 gcagcggcgc aacggcaaat agcaccagcg cctgaattaa cggatataaa acactcattc
    29161 tcaggctcct cgtgaaacaa taatcaccac cagcaccgcc agttcaacca gcgccatccg
    29221 gcggaacagc aacgcactgc cttcgcactg ccagcccggc accagcgaca ccggattcag
    29281 ccatttgcgc agtttcagca ccggcgcaaa cgcctgtttc accggcatgg caaaaccgtg
    29341 ggcggtaatt accattgatt tttcatggtc gtagccgcac acccaggccg cgccgcggga
    29401 acgcgatggc aaacgatcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca
    29461 ggcaatcagc agcaacgtga tcatcggttg agaaacggtg gtgttagcag gttccagcgg
    29521 cagaggtaca gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac
    29581 gcagcaaatc gccagtgcca ctacgcttac gctcatcagg agcggtgcac aggtggcgtt
    29641 ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca
    29701 catcaccgcc agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg
    29761 ggcaacaaac gcgccactat tgctcagttt gaagaaggat tgatagataa cccattcccc
    29821 ggcaaaaccg ttcagcggcg gcagtgcagc cattgccatc agcccgacta acatagcgat
    29881 ggagataacc ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt
    29941 acggaaccag acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag
    30001 cagatggtac agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat
    30061 acccgttacg ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta
    30121 agccagtaga cgctggatat tatgttccat caacgcatac agaccgccga caaacgcggt
    30181 gatcataccg agcaccagca gcgcgatccc ccaccacagc ggtgcattac cgcccagcag
    30241 tgacagggtt aaaatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc
    30301 agctggcgca gaggcgttcg catgtgcctg cggcacccag ccgtgcagcg gaataatccc
    30361 ggccagcagg ccaaagccaa tcacactgag cagccagata tcggaaccga gcggcagctg
    30421 ttgcatacgc atatccagca ggcgcagatc cagcgtgccg taacgctgcc acagcagcca
    30481 gcaagcaatc gccagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc
    30541 tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa
    30601 catgccgagg ttgctggcaa tgacggcgca gacggcggca gccatcaaca tattgatctg
    30661 caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa
    30721 cagaccgcac agaccgagcg taatcagcca aatcgcgtta agcggagaga tttgcacatc
    30781 gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagta cagtgaagcc
    30841 cgcggctgcc gtatacagac taccaaccgc gccgccaatt ccggctatcc agccgcttag
    30901 cgccttttga aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc acgccacgcc
    30961 gctattgatc agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag
    31021 gtgagatcgc caaagtccgt gttaaaggtc agctcacgct tacgtttgct gacgcgggcg
    31081 atatcggtgt tatccaccag atgcagggct ttagtcgggc acatccgcac gcaggccgga
    31141 ccttgttcat caaagctaca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc
    31201 cagtcaagca gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta
    31261 ttggcatttg caggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg
    31321 caggcgatgc cgcacagctt gcagcttacg cacaggcttt cattcaactg cacggcccca
    31381 tcaacgcggg tgatggcgtt aaccgggcag accaccgcgc agggtgcatc ttcacagtgg
    31441 tgacagagct gcggcgcaga ttctttttca tttagcatca ctctcaggcg cggcattgat
    31501 tgcaggccgt gctggcgatg cgtctctgaa caggcggcct cacaagtgtg gcaaccgata
    31561 cagagcgtgg agtcagcaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat
    31621 ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc
    31681 aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc
    31741 ctttaccagt tggcttaaat tcaccggcac attgttttca atagcggtgt ataagctgtc
    31801 gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt
    31861 gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc
    31921 ttcgccatct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag
    31981 ggtgacgtaa atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca
    32041 gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca
    32101 gtagatgtgc cactggtcct gtaggcgacg atgccgctgt gcgatgtaat cggctttctc
    32161 gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc
    32221 attttttgtg ccaactttta atttattatt attaaagcga tttttaagat tttggtgaat
    32281 ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcactg cagggataat
    32341 caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga
    32401 aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc
    32461 aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc
    32521 tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca
    32581 cctcgaagaa caagaggccg aatgctggtg tgaaacatgc caacagtatg tgacgctact
    32641 gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga
    32701 cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg
    32761 cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt
    32821 tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc
    32881 gcctgaattt acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc
    32941 gggcactcac gcaccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct
    33001 ggacaaaaat aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgcct gcaagcaact
    33061 ggtgctcaac ctggtttcca gcccaggttc cggtaaaacc accctgctga cggaaacctt
    33121 aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa
    33181 cgatgccgca cgcattcgcg ctaccggcac accagcgatt caggtgaaca ccggtaaagg
    33241 ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa
    33301 cggtattctg tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg
    33361 tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata
    33421 tccgcatatg tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta
    33481 tctcaacttt gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga
    33541 aatcatcctt atttccgcca ccagcggcga agggatggac cagtggctga actggctgga
    33601 gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg taaccaggcg
    33661 aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat
    33721 gaaaacggtc agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc
    33781 gtaattaatg aagccgaagc acgcgacact ctcgacgcct tgcaaaacat gtttgacgtt
    33841 gagccggatg tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat
    33901 atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc
    33961 tctcttacac cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg
    34021 ccatttttaa attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc
    34081 cgggttgccc ggtgtgcgta ctgccgatgg gcagaatcga cacctgcgtg gagattgcca
    34141 gccatccgga agtcatcttc tgtacctttg gcgacgccat gcgcgtgccg gggaaacagg
    34201 gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtctat tcgccgatgg
    34261 atgcgttgaa actggcgcag gagaatccaa cccgcaaagt ggtgttcttc ggcttaggtt
    34321 ttgaaaccac catgccgacc accgccatca ctctgcaaca ggcgaaagcg cgtgatgtgc
    34381 agaattttta cttcttctgc cagcatatta cgcttatccc gacgctgcgc agtttgctgg
    34441 aacagccgga taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg
    34501 gcactgatgc ctataatttt atcgccagcg attttcagcg tccgctggtg gtggctggtt
    34561 tcgaacccct tgatctactg caaggcgtgg tcatgctggt ggagcagaaa atagcggccc
    34621 acagcaaggt agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc
    34681 aacaggcgat tgccgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg
    34741 tgattgaatc ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac
    34801 atttccgccc ggcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggcgaagtct
    34861 tgacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa
    34921 ccgcgtttgg tgcgctgatg gtttcctccg aaggagcgtg cgccgcgtgg tatcagtatc
    34981 gtcagcagga gagtgaagcg tgaataatat ccaactcgcc cacggtagcg gcggccaggc
    35041 gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga
    35101 gcaggaagat caggcacgtc ttgatctggc gcagctggta gcggaaggcg accgtctggc
    35161 gttctccacc gacagttacg ttattgaccc gctgttcttc cctggcggta atatcggcaa
    35221 gctggcgatt tgcggcaccg cgaatgacgt tgcggtcagt ggcgctattc cgcgctatct
    35281 ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac
    35341 cagcatggca gaaaccgccc gcacggcagg cattgccatc gttactggcg atactaaagt
    35401 ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc gcgggcatgg gcgcaattcc
    35461 gacgaatatt cactggggcg cacagacgct aaccgcaggc gatatattgc tggttagcgg
    35521 tacactcggc gaccacgggg cgactatcct taacctgcgt gagcagctgg ggctggatgg
    35581 cgaactggtc agcgactgcg cggtgctgac gccgcttatt cagacgctgc gtgacattcc
    35641 cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgta aacgcggtgg ttcatgagtt
    35701 cgcggcagcc tgcggttgcg gtattgaaat ttctgaatca gcgctgccgg ttaaacctgc
    35761 cgtgcgcggc gtttgcgaat tgctgggact ggacgccctg aactttgcca acgaaggcaa
    35821 actggtgatc gccgttgaac gcaacgcggc agagcaagtg ctggcagcgt tacattccca
    35881 tccactgggg aaagacgcgg cgctgattgg tgaagtggtg gaacgtaaag gtgttcgtct
    35941 tgccggtctg tatggcgtga aacgaaccct cgatttacca cacgccgaac cgcttccgcg
    36001 tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg
    36061 cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag
    36121 ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg
    36181 ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc
    36241 aagcgcagac tcaacgtgcg tcttattacg catcgcgtga aaaagacacc cccattaaat
    36301 atgaagacga aactgttctg gcacacggtc cggtacgcag cattttgtcg cgccctgata
    36361 cgttgcattg cagttacgaa gaattttgtg aaacctggcc gcagctggcc gcaggtgggc
    36421 tatacccaaa atttggtcac tattgcctga tgccactggc ggcggaaggg catatttttg
    36481 gtggctgtga atttattcgt tatgacgatc gcccctggag cgaaaaagag ttcaatcgtc
    36541 tgcaaacatt tacgcagatc gtttctgtcg tcaccgaaca aatccagagt cgcgtcgtta
    36601 acaatgtcga ctatgagttg ttatgccggg aacgcgataa cttccgcatc ctggtcgcca
    36661 tcaccaacgc ggtgctttcc cgcctggata tggacgaact ggtcagcgaa gtcgccaaag
    36721 aaatccatta ctatttcgat attgacgata tcagtatcgt cttacgcagc caccgtaaaa
    36781 acaaactcaa catctactcc actcactatc ttgataaaca gcatcccgcc cacgaacaga
    36841 gcgaagtcga tgaagccgga accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc
    36901 tgattaatct ccacgagcgg gatgatttag ccccctatga acgcatgttg ttcgatacct
    36961 ggggcaacca gattcaaacc ttgtgcctgt taccgctgat gtctggcgac accatgctgg
    37021 gcgtgctgaa actggcgcaa tgtgaagaga aagtgtttac cactaccaat ctgaatttac
    37081 tgcgccagat tgccgaacgt gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa
    37141 tccatcgtct gaaagaacgg ctggttgatg aaaacctcgc cctgaccgag cagctcaaca
    37201 atgttgatag tgaatttggc gagattattg gccgcagcga agccatgtac agcgtgctta
    37261 aacaagttga aatggtggcg caaagtgaca gtaccgtgct gatcctcggt gaaactggca
    37321 cgggtaaaga gctgattgcc cgtgctatcc ataatctcag tgggcgtaat aatcgccgca
    37381 tggtcaaaat gaactgcgcg gcgatgcctg ccggattgct ggagagcgat ctgtttggtc
    37441 atgagcgtgg ggcttttacc ggtgccagcg cccagcgtat cggtcgtttt gaactggcgg
    37501 ataaaagctc cctgttcctc gacgaagtgg gcgatatgcc actggagtta cagccgaagt
    37561 tgctgcgtgt attgcaggaa caggagtttg aacgcctcgg cagcaacaaa atcattcaga
    37621 cggacgtgcg tctaatcgcc gcgactaacc gcgatctgaa aaaaatggtc gccgaccgtg
    37681 agttccgtag cgatctctat taccgcctga acgtattccc gattcacctg ccgccactac
    37741 gcgagcgtcc ggaagatatt ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc
    37801 gtctggggcg caatatcgac agcattcctg ccgagacgtt gcgcaccttg agcaatatgg
    37861 agtggccggg taacgtacgc gaactggaaa acgtcattga gcgcgcggta ttgctaacac
    37921 gcggcaacgt gctgcagctg tcattgccag atattgcttt accggagcct gaaacgccgc
    37981 ctgccgcaac ggttgtcgct caggagggcg aagatgaata tcagttgatt gttcgcgtgc
    38041 tgaaagaaac taacggcgtg gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga
    38101 aacgcacgac cctgctgtca cggatgaagc gactgggaat tgataaatcg gcattgattt
    38161 aactgcaaat tgccggacag atctgcctgt ccggcatact attcacgagg ttttttcgga
    38221 cgatattttt ccggcagttc tggcaccgga cacttgtcat cgatgagatg acgcacggtt
    38281 aagatcggat gacgccacag cattctcggc ccggcccaac gcataatctg tttcatctct
    38341 tcacgctttg caggctggta acagtgcacc ggacactgct tacaggctgg tttctcttcg
    38401 ccgaacacac atttatccag ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc
    38461 ggctccgctg acgcctgcgg gcatttcgct tgataaagat cgatcatttt tttaatcgtc
    38521 agtttttcac gagagatacg cttgccggac atactgcctc cacctcatta agatgcattt
    38581 atattacaac ttaatcttaa agggcactat gactctaaag aagaagggtt agccaaccga
    38641 tacaattttg cgtacttgct tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc
    38701 cggaaccagc atcaagaact cgccgttcgc ttcttcccct gaaatgatta actccggtat
    38761 catgtgcgcc ttatgtgatt acaacgaaaa taaaaaccat cacaccccat ttaatatcag
    38821 ggaaccggac ataactccat gagtgcaata gaaaatttcg acgcccatac gcccatgatg
    38881 cagcagtatc tcaagctgaa agcccagcat cccgagatcc tgctgtttta ccggatgggt
    38941 gatttttatg aactgtttta tgacgacgca aaacgcgcgt cgcaactgct ggatatttca
    39001 ctgaccaaac gcggtgcttc ggcgggagag ccgatcccga tggcggggat tccctaccat
    39061 gcggtggaaa actacctcgc caaactggtg aatcagggcg agtccgttgc catctgcgaa
    39121 caaattggcg atccggcgac cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt
    39181 acgccaggca ccatcagcga tgaagccctg ttgcaggagc gtcaggacaa cctgctggcg
    39241 gctatctggc aggacagcaa aggtttcggc tacgcgacgc tggatatcag ttccggtcgt
    39301 tttcgcctga gcgaaccggc tgaccgggaa acgatggcgg cagaactgca acgcactaat
    39361 cctgcggaac tgctgtatgc agaagatttt gctgaaatgt cgttaattga aggccgtcgc
    39421 ggcctgcgcc gtcgcccgct gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat
    39481 ctgcaatttg ggacccgcga tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt
    39541 tgtgctgccg gttgtctgtt gcagtatgcg aaagataccc aacgtacgac tctgccgcat
    39601 attcgttcca tcaccatgga acgtgagcag gacagcatca ttatggatgc cgcgacgcgt
    39661 cgtaatctgg aaatcaccca gaacctggcg ggtggtgcgg aaaatacgct ggcttctgtg
    39721 ctcgactgca ccgtcacgcc gatgggcagc cgtatgctga aacgctggct gcatatgcca
    39781 gtgcgcgata cccgcgtgtt gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc
    39841 accgccgagt tgcagccggt actacgtcag gtcggcgacc tggaacgtat tctggcgcgt
    39901 ctggcgttgc gtaccgctcg cccacgcgat ctggcccgta tgcgtcacgc tttccagcaa
    39961 ctgccggagc tgcgtgcgca gttagaaact gttgatagtg caccagtaca ggcgctacgt
    40021 gagaagatgg gcgagtttgc cgagctgcgc gatctgctgg agcgagcaat catcgacaca
    40081 ccgccggtgc tggtacgcga cggtggtgtt atcgcatcag gctataacga agagctggat
    40141 gagtggcgcg cgctggctga cggcgcgacc gattatctgg agcgtctgga agtccgcgag
    40201 cgtgaacgta ccggcctgga cacgctaaaa gttggcttta atgcggtgca cggctactac
    40261 attcaaatca gccgtgggca aagccatctg gcacctatca actatatgcg tcgccagacg
    40321 ctgaaaaacg ccgagcgcta catcattcca gagctaaaag agtacgaaga taaagtcctc
    40381 acttcaaaag gcaaagcact ggctctggaa aaacagcttt atgaagagct gttcgacctg
    40441 ctgttgccgc atctggaagc gttgcaacag agcgcgagcg cgctggcgga actcgacgtg
    40501 ctggtgaacc tggcggaacg ggcctatacc ctgaactaca cctgcccgac cttcattgat
    40561 aaaccgggca ttcgcattac cgaaggccgc catccggtgg ttgaacaggt gctgaacgag
    40621 ccatttatcg ccaacccgct gaatctgtca ccgcagcgcc ggatgttgat tattaccggt
    40681 ccgaacatgg gcggtaaaag tacctatatg cgccagaccg cactgattgc gctgatggcc
    40741 tatatcggca gctacgtacc ggcgcaaaaa gtcgagattg gcccgattga ccgtatcttt
    40801 acccgcgtag gggcagcgga tgatctggct tccgggcgtt caacctttat ggtggagatg
    40861 accgaaaccg ctaatattct gcataacgcc accgagtaca gtctggtgct gatggatgag
    40921 attgggcgcg gaacgtccac ttacgatggt ctgtcgctgg cgtgggcgtg cgcggaaaat
    40981 ctggcgaata agattaaggc gttgacgctg tttgccaccc actatttcga gctgacccag
    41041 ttaccggaga aaatggaagg cgtcgccaac gtgcatctcg atgcactgga gcacggcgac
    41101 accattgcct ttatgcatag cgtgcaggat ggcgcggcga gcaaaagcta cggcctggcg
    41161 gttgcagctc tggccggcgt gccaaaagag gttattaagc gcgcacggca aaaactgcgt
    41221 gagctggaaa gcatttcgcc gaacgccgcc gctacgcaag tggatggtac gcaaatgtct
    41281 ttgctgtccg taccggaaga aacctcgcct gcagtcgagg cactggaaaa cctcgatccg
    41341 gattcactca ccccgcgtca ggcgctggaa tggatttatc gcctgaagag tctggtgtaa
    41401 taataattcc cgatagtctt ttgctatcgg gaatattaac gataactgac gaatcaaata
    41461 aaaataccct gtataatagg aaagcttatt ttacagggta aaaccatgcc atctacacgc
    41521 tatcaaaaaa tcaataccca tcactatcgc catatatggg tcgttggtga tattcatggt
    41581 gaatatcagt tattacaatc ccgcttacat caactctctt ttttccccga aaccgactta
    41641 cttatttctg tcggcgataa tattgatcgt gggccggaga gtcttaacgt cctgcgcctg
    41701 ctaaaccagc cctggtttat ctccgttaaa ggcaaccacg aagcaatggc gctggatgcg
    41761 ttcgcgaccg gcgatggcaa tatgtggctt gccagcggtg gtgactggtt tttcgattta
    41821 aatgattcag agcaaaaaga agctacagat ctgttgctga aattccatca ccttccacat
    41881 attattgaaa tcactaacga caacataaaa tatgtcatcg cacatgcaga ttatccgggg
    41941 agtgaatatc tctttggtaa agaaatagcg gagagcgaat tactctggcc tgttgatcgt
    42001 gtgcagaaat cgcttaatgg cgagttacaa caaataaacg gcgctgatta ttttatattt
    42061 ggacatatga tgtttgataa cattcagacg ttcgctaacc agatttatat tgataccgga
    42121 tcgccgaaaa gcgggcggct gtcattttat aaaataaagt aatgaggccg ggtaaagcaa
    42181 agccactacc cggcactttt tattagcgct taccttccgc cagcagcggc gggatgctcg
    42241 gcaccattgg cgcgtcatca atatctttct gcgtcatgcg gaacgcttcg ggataatgtt
    42301 cgcggctggt acggcgcagc ggttcggtat cgcgccaggt ataaaggcaa tgctggcact
    42361 gatataccgt ccagacatct ttcaccggcg atttcgccat cacttcaatc tgttcatcgg
    42421 cacaacgtgg acaaatcatc ttgttctcct tatttacgtg cggccagcat agcggtcagt
    42481 ttttcagccc aggctttggt ttccggcaaa tccaccaccg gctggctgta gtgaccacgg
    42541 ttgtccgggg cgacaggcgt ggtggcgtcg ataatcagct tgtcggtgat ccccgccggg
    42601 cttgagccag ggtcgagttc cagcacggac atattcggca actgcaccaa atcccctgcc
    42661 ggatttactt tcgaggagag cgcccacatc acctgcggca ggttgaacgg gtcaacgtct
    42721 tcatcgacca taatcaccat ctttacgtag cccagaccgt gcggcgtggt catcgcacgc
    42781 aggcccaccg cgcgggcaaa gccgccgtag cgttttttgg tggagataat cgccagcagg
    42841 ccgtgggtgt acatggcgtt taccgcctgc acttccggga actcggcttt cagttgttga
    42901 tacagcggca cacaggtggc tggccccatc aggtagtcga tttcggtcca cggcatgccg
    42961 aggtacagcg attcgaaaat cggcctggtg cggtaagaga ctttatcgat acgcaccacg
    43021 gtcatgttac gcccgccgga gtagtgcccg gtaaactcac cgaacggccc ttcgatttcg
    43081 cgtttgcggc tttcgataac cccttcgagg atcacttctg aaccccacgg cacatcaaaa
    43141 ccagtcaatg gcgcggtggc gatcgggtac gggctttcgc gcagcgcgcc tgccatttca
    43201 tactcagact gatcgtattt cagcggcgtg gcgcccataa gggtgatgat cggatcgtta
    43261 cccagggtga tggcaatcgg cagatcttca ccgcgctctt ccgctttatg cagatgcagg
    43321 gcgatatcgt gcatcggcac cggttgcagg ccgagcttac gcttgccctt cacttccatg
    43381 cggtagatac cgacgttctg cttgccgaag ttatccgggt cgagcggatc gcgggaaacc
    43441 acgcacgctt tgtcgagata gaaaccgcca tcaccgtcgt ttaaacgaaa cagcggcagg
    43501 atatcgaaca gattaatctc gtcaccatcg acggtgttct gcgcccaggc tggattggcg
    43561 cggcgctccg gggcgatcgg gaagttatcc cagcggcgga taaactcatc aatctgtttt
    43621 ttaaccgggg tgtttggcgg caggcccaag gaaatcgcgt ggttctgcca ggaaccgatg
    43681 gtattcatcg ccacgcgggc atcggtaaaa ccgcgaatat tatcaaacca cagcgccggt
    43741 gcaccatcgc cgatacgccc ggtggcgttg gcagcggcag ccagatccgg ttccgcgttc
    43801 acctcttcgc tgattttcag taactggccg tggtcatcaa gcgcctgtaa aaagctgcgt
    43861 aaatcatcaa atgccattat tcattctcct gagaaaaatt ccgggcctgc ggcaatcctt
    43921 gccagcgcct ggcgtaggga tgttcaaggc caaattgatc cagcacgcgg gctaccacgt
    43981 ggtggacaat gtcatctacc gtttcgggat ggttataaaa ggcaggcatc ggcggcacca
    44041 tcgccacgcc catgcgtgaa agtgcgagca tattttcgag atggatggtg ctaagcggca
    44101 tttcacgcgg caccagcacc agtttgcggc cttctttaag cacgacgtcc gccgcgcgcc
    44161 ctaccaggcc atcagcgtaa ccagcgcgga taccggcgag cgttttcata ctgcacggaa
    44221 taacgatcat gccgtctgta cgaaaggaac ctgaggagat ggtcgccgcc tgatccgccg
    44281 ggttatggct gaagtcagcg agggcagcaa catcgcgggc gctgtaaggc gtttccagtt
    44341 caatggtggt tttcgcccac ttcgacatca ccagatgagt ctcgacattc ggcatctccc
    44401 gcagcgcttg cagtaatgcc acaccaagag gcgcaccggt agcccctgtc atcccgacga
    44461 tcagtttcat tgcttactct cttaattgtt cgcatacgaa cattattatt aaaatacgcc
    44521 tctgttctca ttgcggcaag cactgaaaag taacaacatc cccttcgtca ctgaaaaacc
    44581 gctatgatag cggcagatag tttgcccgga gtttacatga cgttacgaaa taaagcgttc
    44641 catcagttac gacagctttt tcagcagcac acggctcgct ggcagcacga gttacctgac
    44701 ctgaccaaac cacagtatgc ggtgatgcgc gccattgctg ataagcctgg tatcgaacag
    44761 gtggcactga tagaagcagc agtcagcacc aaagcaacgt tggcagaaat gttggcaaga
    44821 atggagaatc gtggcttagt cagacgtgaa catgatgccg ctgacaagcg gcgtcgcttt
    44881 gtctggctga ctgccgaagg agaaaagata cttgctgcgg cgataccgat tggtgacagc
    44941 gtggatgagg aatttttggg gcgtttgagt gcagaagagc aggagctgtt tgtgcagctg
    45001 gtgcgcaaga tgatgaacac ataggatgca aattgccggg taggacgctg acgtgtctta
    45061 tccaggcgac aaaaacacaa gcttcccaaa caaaagaaaa aggccagcct cgcttgagac
    45121 tggcctttct gacagatgct tacttactcg cggaacagcg cttcgatatt cagcccctgc
    45181 gtttgcagga tttcgcgcaa acggcgcagg ccttcaacct gaatctggcg aacacgttca
    45241 cgggtgaggc caatttcacg acctacatct tccagtgttg ccgcttcgta ccccagcaaa
    45301 ccgaatcgac gtgccagtac ttcacgctgt ttggcgttca gctcgaacag ccatttgacg
    45361 atgctctgct tcatatcgtc atcttgcgtg gtatcttccg gaccgttctc tttttcatcg
    45421 gccaggatgt ccagcaacgc tttttcggaa tcaccaccca gcggggtgtc taccgaggta
    45481 atgcgctcgt taagacgaag catacggctg acgtcatcaa ctggcttatc cagttgctct
    45541 gcgatctctt ccgcacttgg ttcatggtcc agcttatggg acaactcacg tgcggttcgc
    45601 aggtaaacgt tcagctcctt tacgatgtga atcggcaaac gaatagtacg ggtttggttc
    45661 ataatcgccc gttcaatcgt ctggcgaatc caccaggttg cgtatgttga gaagcggaaa
    45721 ccacgttccg ggtcaaactt ctctaccgca cggatcagcc ccaggttgcc ctcttcgata
    45781 aggtccagca acgccagacc acgattgcca taacggcggg caatttttac caccagacgc
    45841 aagttactct cgatcatccg gcggcgagag gcgacatctc cacgcagtgc gcgacgcgca
    45901 aaataaactt cttcttcggc cgttaacagt ggtgaataac caatctcacc aaggtaaagc
    45961 tgagtcgcgt ccaacacacg ctgtgtggct ccctgcgata acagttcctc ttcggccaaa
    46021 tcgttatcac tgggttcctc ttctactaag gccttttcgt caaaaacctc aactccgttc
    46081 tcatcaaatt ccgcatcttc atttaaatca tgaactttca gcgtattctg actcataagg
    46141 tggctcctac ccgtgatccc ttgacggaac attcaagcaa aagcctggtt ccgccgattt
    46201 atcgctgcgg caaataacgc agcgggttta cggatttccc cttgtaacga atttcaaaat
    46261 gcaagcgtgt tgaactggtt ccggtgctac ccatggttgc tattttttgc cccgccttca
    46321 cttcttgttg ttcccggacc agcattgtgt cgttatgggc gtaggcactc aggtaatcat
    46381 cattatgttt gatgataatc agattaccgt agccgcgcag cgcgttaccg gcataaacaa
    46441 cacggccatc tgcggtcgcg ataattgcct gtcctttgct gcctgcgata tcaatcccct
    46501 tgttgccccc ctcagaagcg ccaaaggttt cgatcacttt gccctcagtc ggccagcgcc
    46561 aggtggagat aggcgtactg gttgatgtac tgctgacagt cggctcggtt gtgcttgctg
    46621 ttggtaccgt tacaggcgct gtgaccgtgg tcgcagttgg cttgttgttc ggcaacattt
    46681 tgttagcact ctgttcaccc gaagactcag aatacgtaat tgtcggttgc gacgcaacag
    46741 caacggtgga attttgtgca ggcttgatca caactccttg ctctgctgcg tcggcctggg
    46801 taatggcatt tccgccagtg attggcgtac cggaagcatt acccacctgt aaggtctgac
    46861 cgacgttcag cgcgtatggt gcctgaatat tgttgcgctg agcaaggtca cggaaatcgt
    46921 tgccagtaat ccaggcgata tagaaaagcg tgtcgccttt tttcacggta taggtactgc
    46981 cgctataact gcctttcgga atgttcccat actgacggtt atagacgatg cgtccgtttt
    47041 ccatctgtac cggctgctga gctactggct gcactggctg gatttgcggt tgttgagtag
    47101 cctgaatttg tggctgctgc accggctgaa tttgcggttg ctgcgctgta gacgtcgtcc
    47161 ccattttcgg cggcggcgta atcaacatac cagaattggt atgtgcaggc gcattgccat
    47221 taacggagct gaccggtgcc ggtggatttg aagtgtcaga acagcctgcc agccatagcg
    47281 aaaccagtga caaagccgca atgcggcgaa cggtgaattt tgggcttccc gcgctcattt
    47341 atcccccagg aaaaattggt taataaccag tgacataatt accgtgcaag gcaccatact
    47401 gaacactgga aaagatgttc acgatacgct gacctgcggc aaaataacca ggaaaaatcc
    47461 aggtatttcc tcacgtttta agccagctca cccttcacta aagggacaaa gcgcacggcc
    47521 tccacggtat cgataataaa ttcgcctccc cgacgacgca cccgtttcaa atactggtgc
    47581 tcctccccta cgggtaagac gagaatcccg ccttcgtcca gctgcgtcat tagcgcagtt
    47641 ggaatttccg gcggtgccgc cgtaacaatg atagcgtcaa acggcgcacg tgcctgccaa
    47701 ccttgccatc catcgccatg acgggttgaa acattatgta aatcaagatt tttcaggcgg
    47761 cgacgcgcct gccactgcaa gcctttaatc cgttcaaccg agcaaacatg ctggacaaga
    47821 tgcgccagga ttgccgtttg atatcccgaa ccggtgccaa tttccagcac ccgcgactgc
    47881 ggcgtcagct cgagtaattc ggtcattcgc gccaccatat acggctgcga aatcgtctgc
    47941 ccctgaccta tcggcaaggc gatattgtcc caggcttttt gttcaaacgc ttcatcaacg
    48001 aatttttcac gcggcacggc ggcaagtgca ttcagcacct gctcatcctg aataccttgc
    48061 gcacgtaatt gatccagaag tgcttgtacg cgtctgctta ccattgcgtg ccaactccca
    48121 cgctgtttaa ccagtctgaa accacatctt gcgcgctatg cgcagttaaa tccacatgca
    48181 gcggcgtgat ggagacatag ccctcatcta ccgcagcaaa atcggtcccc ggaccggcat
    48241 cacatttacc gcccggcggg ccaatccagt acagcgtatt gccgcgcgga tcttgctgcg
    48301 ggatcacctg atctgccgga tgtcgtgtac cgcagcgcgt cacgcgaata cctttgattt
    48361 gatccaaggg taaatccgga acattaatat taagaatacg cccggtgcgc agcggctctt
    48421 tacacagtgc gcgcaaaatt gaacaggtta ccgccgcggc agtgtcgtaa tgtttatgcc
    48481 cgtcaagcga gacggcaagc gccggaaaac ctaaatgacg gccttccatc gcggcggcta
    48541 ccgtaccgga ataaataaca tcatccccca gattcggccc ggcgttaatt ccggacacaa
    48601 caatgtccgg gcgcggacgc atcagagcat tcacgccaag atagacgcaa tcggtcgggg
    48661 ttcccatttg cacagcaata tcaccatttt caaaggtaaa cgtgcgcagg gaggattcca
    48721 gtgtcagaga atttgaagcg ccgctgcggt tacgatcggg ggcgaccacc tgaacgtcag
    48781 caaactcacg caaggctttc gccagcgttt gtataccggg tgcatgtacc ccgtcatcat
    48841 tactcagcaa tatgcgcata atcacctgtt gtgttgataa gttccctgac aacgctggtt
    48901 gcaaaactac ccgccggaag ccagaaacgg atttctacgg tgacgtcatc ccaccaattc
    48961 cagcttaatt gttgcggata cagcagcatc gctctgcgcg cggcttcaac tttttcgcgc
    49021 accagtaaag cttgtaattc agtttctgcg gcgacagctg cctgttcgaa tgccagcgct
    49081 tcacgctgag ttccccattc accactgccc ggcaatgcgg cggttatcat cagctcttta
    49141 tcgttgacgc gacgctgtaa ttccaccagt tcttcggtgg ttgcaacaaa ccagctacca
    49201 cgtccggcta attgtagcgc atcgccgtca acaacttgat taacgtctgc ttttttgagg
    49261 cgctcagcaa caatctgatt aaacaacgca ctgcgggctg ccgacaacca aaaactccgt
    49321 ttattgcgat cgcgcaccgg agtattggtt tgcgcccagc gcagcgcgcc ctgcaagttg
    49381 ctaccgccaa tcccaaaacg ttgggcaccg aagtagttcg gtacaccttt tacgcaaata
    49441 tcgatcagac gttgttcaac gtcatcgcga ttgctcactt cgcgcagaac cagggtaaag
    49501 gcgttacctt tcagcgcccc taaacgcagc ttgcgcttgt gccgcgcata ctccagcacc
    49561 tggcagcctt ccaattgaaa ggcgctcaga tcgggcattt ccttgcccgg cacgcgagcg
    49621 cataaccact gttctgtaac agcatgtttg tctttttgcc cagcaaagct gacttcacgg
    49681 gcatgaattt tcaggaattt cgccagtgca tccgccacaa aacgggtatt gcagccgttt
    49741 ttgaggattc taaccagaat atgctcacct tcaccatcag gctcaaagcc caaatcttcc
    49801 accaccacaa agtcttccgg attggctttc agcagcccgg tgccttgcgg tttaccgtgg
    49861 aggtaagtga gattatcaaa ctcaatcatt ttgttgcctt aatgagtagc gccaccgctt
    49921 cacaggcaat cccttcccca cgtccggtaa atccaagttt ttccgtagta gtggctttca
    49981 cgttaacatc atccatatgg cagccgagat cttcggcaat aaacacgcgc atttgtggaa
    50041 tgtgcggcaa catcttcggt gcctgagcga tgatagtgac atcgacgttg ccaagggtat
    50101 aacccttcgc ctgaatacga cgccaggctt cgcgtagcag ctcgcggcta tcggcacctt
    50161 taaatgccgg atcggtatcc gggaacagtt tgccgatatc ccccagcgcc gccgcgccaa
    50221 gcaatgcatc ggtcaacgca tggagcgcca cgtcgccatc agaatgcgcc agcaatcctt
    50281 tttcgtaagg aatgcgtacg ccaccaatga taattgggcc ttcaccgcca aaggcgtgta
    50341 cgtcaaaacc gtgtccaatt cgcattatgt attctcctga tggatggttc gggtgaggta
    50401 aaactcggcc agtgccaaat cctccgggcg cgtgacttta atgttatccg cacggccttc
    50461 gaccaactga ggatggaatc cgcaatattc cagcgccgag gcttcgtcgg taatagccgc
    50521 gccttcattt agagcgcgcg tcagacagtc atgtaacagc tcacgaggga aaaattgcgg
    50581 cgtcagcgcg tgccataagc cgttgcgatc aacggtatga gcaatggcat ttttgcccgg
    50641 ttcggcacgt ttcatagtat cgcgcactgg tgcggctagg atccctcccg tgcggctggt
    50701 ttcgctcaac gccaacaatc gcgcgaggtc atcctgatgc agacaaggac gagcggcgtc
    50761 atgcaccaat acccactgcg cgtcgccagc ggctttcaaa cctgccagca cggaatcggc
    50821 acgctcatca ccgccatcta caacggtgat ttgcggatga ttcgccagag gaagttgtgc
    50881 aaaacggcta tcgccaggac ttatggcaat gacgacacgt ttcacccggg gatgcgccag
    50941 cagcgcatac accgagtgtt caagaatggt ttgattaccg attgagagat attgcttagg
    51001 acattccgtt tgcattcgac ggccaaatcc ggccgccgga accacggcgc aaacatccaa
    51061 atgagtggtt gccatgttaa ttcccgggct gatttatcga ttgttttgcc ccgcagactg
    51121 tgcgcgcttc gacgcgtcag gcaccagacg ataaaaagtt tcgcccggcc tggtcatgct
    51181 gagttcatta cgcgcacgct cttcgagcgc ctcctggccg ccattgagat cgtcaatttc
    51241 ggcaaaaagt tgatcgtttc gcgctttaag tttcgcgttt gtagcttgct gtgccgccac
    51301 atcatcattg acgcgggtat agtcatgtat accgttctta ccgaaccaca gcgaatactg
    51361 tagccagacc agaatagcca gcaacagcag cgttagttta cccatcctgc cccctgaaaa
    51421 acggcatcat catcccatgc atccgaagac gactctacat cctctgttgg ggataccgcg
    51481 acaacgcggg caaatgtacc acatttgtcc attgttacgt atacccaggg cgtgcagaac
    51541 ataatctcat tattagttac ggtttgaatt atgaacagag gagacaagaa agtacaaatt
    51601 agcccagtag ccacataaac agtgcgccaa acataatgcc tactgtcatc agggtgaaaa
    51661 caatactgta gcgtagcttt ccgtccatca atgaatgcag cgcaatcccc accactaccg
    51721 caacgggcat cagcgccaga aagaaaggcc aggtgtagat aaagaagaac agcgtgttag
    51781 agccataaat caacatcggc atcgccagcg caaataacca ggagataaaa ccgaccacgg
    51841 caccaggcag tgaccatgtg gtttcttcat cctcagtaag gctgtcgtta tttgttagtg
    51901 taatgttatg gctattacgc atatttgatc ctgttacttt gacgaaccgg gcatggaaac
    51961 ccggtggtgt ctcaggatct gataatatcg ttctgtctca acagatctaa taattgctgt
    52021 accaaatttg ttactaattg ttcaccattg agatgaattt ctgccgattc aggcgcttcg
    52081 taaacggaat ctattcccgt aaagttgcgc agttcaccgg cacgcgcttt cttatataag
    52141 ccttttggat cgcgggcttc gcaaatcgcc agcggcgtat cgacaaacac ttcgataaag
    52201 cgcccttctc ctacgcgttc gcgaaccatc tggcgttcgg cgcggtgtgg cgagataaat
    52261 gcggtcagca ccaccagtcc ggcttcaacc atcaaattcg ccacttcacc gacgcgacgg
    52321 atattctctt tacgatcggc atcgctaaaa ccgagatcgc tgcataatcc gtggcgaaca
    52381 ttgtcgccat ccagcagata cgtactgacg ccgagtttat gtaacgcctc ctccagcgcc
    52441 ccggcgaccg ttgatttacc ggacccggag aggccggtaa accacagcac tacaccacga
    52501 tgaccgtggt gtagctcgcg ttgttgcaca gtgaccggat ggctatgcca gacgacgttt
    52561 tcgtcatgca gcgccattat ttctccccca gcaaatcgcg cgcgccccag tgtggaaagt
    52621 ggcggcgaac cagggcattc aattccagtt cgaatgcact aaattcagat ggcgcagcag
    52681 ttgcctggct aactggctca tgcaccatac cggcacctac ggtcacattg ctcaggcgat
    52741 cgataaaaat cagcccaccg gtaaccgggt tttgctgata acgatctaac accagtggct
    52801 cgtcaaaagt gagatccacc aggccgatgc cgttcagcgg caggttttca acttcgcgtt
    52861 gggtaaggtt attaatatca acctgataac gaatgccatc aacacgagaa cgcgtcttct
    52921 taccggcaat tttgatgtcg taactctggc ccggggaaag cggctgttcc gccatccata
    52981 ccacatccac cgacgcgctc tgcacagctg gtaacgcttc gtctgccgcc agcagcagat
    53041 cgccacggct gatgtcgatc tcatccgtca gcaccagggt gatagcttct ccggcaaagg
    53101 cttcttcgcg atcaccatca aaagtcacga tccgcgcgac gtttgattcc acaccagagg
    53161 gcagcacttt tacacgttgc ccgacttcca cgcgaccgga tgccagcgtt ccggcgtagc
    53221 cacgaaaatc gagatttggg cggttaacgt actgcaccgg gaagcgcatt ggctgggcat
    53281 ccaccactcg ctgaatctcc acggtttcca gcacttcgag cagtgtcgga ccgctgtacc
    53341 acggcatact ttcactttgc gaagccacgt tgtcaccttc cagtgcggag agcggcacaa
    53401 agcggatatc cagattaccc ggcagctgcc cggcaaaggt cagataatct tcacgaatac
    53461 gggtgaacgt ctcttcactg taatccacca gatccatttt gttgatcgcc acgaccagat
    53521 gtttgatccc caacagtgtg gagataaaac tgtgacgacg ggtttgatcg agcacgcctt
    53581 tacgggcatc gatcagtaag atcgccagtt cacatgtcga tgcgccagtc gccatattgc
    53641 gggtgtactg ctcgtgccct ggagtgtcgg cgataataaa tttacgcttc tcggtagaga
    53701 aatagcggta ggccacgtca atggtgatgc cctgttcacg ctcagcttgc aggccgtcca
    53761 ccagcagagc cagatccagc ttttcgccct gggtgccgtg acgcttactg tcattatgca
    53821 gcgatgagag ctgatcttca tagatttggc gggtatcgtg cagcagacga ccaatcaggg
    53881 tacttttgcc gtcatcgacg ctaccacagg tcagaaaacg cagcaggctt ttatgttgtt
    53941 gcgcaatcat ccaggcttcg acgccgcctt cattggcgat ttgttgtgca agtgcggtgt
    54001 tcatcttaaa aatacccctg acgttttttc agctccatag aaccagcttg gtcgcggtca
    54061 atcacgcgcc cctgacgttc actggtggtg gaaaccagca tctcttcgat gatctccggc
    54121 agcgtttgtg catttgactc caccgcaccg gtcagcggcc agcagcccag cgtacggaaa
    54181 cgcaccatcc gttttttaat cacttcgccc ggttgcaggt cgatacggtt gtcatcaatc
    54241 atcatcaaca taccgtcgcg ttccagaacc ggacgttccg cagcgagata cagcggcaca
    54301 atgtcgatat tttccagcca gatgtattgc cagatatcct gctcggtcca gttagagagc
    54361 gggaaaacgc ggatgctttc gcctttgtta atctgcccgt tatagttgtg ccacagctcc
    54421 gggcgctgat ttttcgggtc ccagcgatgg aagcgatcac ggaaagagta gatacgctct
    54481 ttagcacggg atttctcttc gtcgcggcgc gcaccaccga aggcggcatc aaaaccgtat
    54541 ttattcagcg cctgcttcag gccttcggtc ttcataatat cggtatgttt cgcgctgccg
    54601 tgcacgaatg gattaatccc catcgccacg ccttccgggt tttgatgcac cagcagctcg
    54661 cagccgtagg ctttcgccgt acgatcgcgg aactcataca tctcacggaa tttccagccg
    54721 gtatcgacat gcagcaacgg gaaaggcagc gtacctggat aaaacgcctt gcgcgccaga
    54781 tgcagcatga cgctggaatc tttaccgata gagtagagca tcaccggatt tgagaattct
    54841 gccgccacct cgcgaataat gtggatgctt tccgcctcca gttgccgcag gtgagtaagt
    54901 cgtatttgat ccataaccgt tcctttgcaa taccgctatt ttcttgccat cagatgtttc
    54961 gactataggg agcgtaagag aacgaatgaa attaccaatt agaatgagta gttccttaac
    55021 ggaataacga tttggcaaag ctaatatcaa aaagtgctta aggcaccgga tttcgggcgt
    55081 ttaggaagat ttgaaattgt tttagcgcag cggcagtttc atactatggc ggtaaaaaaa
    55141 tttgcatggt atttaaggac tcactatgtt ttccgcattg cgccaccgta ccgctgccct
    55201 ggcgctcggc gtatgcttta ttctccccgt acacgcctcg tcacctaaac ctggcgattt
    55261 tgccaataca caggcgcgac atattgccac tttctttccg ggacgaatga ccggaacacc
    55321 cgcagaaatg ttatctgccg attatattcg gcaacagttt cagcaaatgg gttaccgcag
    55381 tgatattcgt acgtttaata gccgatatat ttataccgcc cgcgataacc gcaaaaactg
    55441 gcacaacgtg acgggaagta cggtgattgc cgctcatgaa ggcaaagcgc cgcagcagat
    55501 cattattatg gcgcatctgg atacctatgc cccgcagagc gacgcagatg cagatgccaa
    55561 tctcggcggg ctgacgttac aaggaatgga tgataacgcc gcaggtttag gtgtcatgct
    55621 ggaactggca gaacgcctga aaaatacgcc taccgagtat ggtattcgat ttgtggcgac
    55681 cagtggagaa gaggaaggga aattaggcgc tgagaattta ctcaagcgga tgagtgacac
    55741 cgaaaagaaa aatacgctgc tggtgattaa tctcgataac ttaattgttg gcgataaatt
    55801 gtatttcaac agcggtgtaa aaacccctga agcagtaagg aaattaacgc gcgacagggc
    55861 gctggcaatt gcgcgtagtc atggaattgc cgcaacgacc aatccgggtt tgaataaaaa
    55921 ttatccgaaa ggcactggat gttgtaatga cgcagaaata ttcgacaaag cgggcattgc
    55981 tgtactttcg gtggaagcga caaactggaa tcttgggaat aaagatggtt atcagcaacg
    56041 cgcaaaaaca gccgcattcc ctgcgggaaa tagctggcat gacgtaagac tggataatca
    56101 gcaacatatt gataaagcac ttcctggaag aatagaacgt cgctgccgtg acgttatgcg
    56161 gataatgcta ccgctggtga aggagttggc gaaggcgtct tgatgggttg gaaaatggga
    56221 gctgggtgtt ctaccgcagg ggcggggaat tctaagtgat atccatcatc gcatccagtg
    56281 cgcccggttt atccccgctg atgcggggaa cacagcggca cgctggattg aacaaatccc
    56341 tgggccggtt tatccccgct ggcgcgggga acacaaaccg aaacacacga tcaatccgaa
    56401 tatgagcggt ttatccccgc tggcgcgggg aacactttgg tgacagtttt tgtcactgtt
    56461 ttggtgacgg tttatccccg ctggcgcggg gaacacacta agcatacata tctgttttta
    56521 aacaaattta ttccacatca acaatctacc aactaaattc aaacatttcc ttatttttaa
    56581 agaacacata acctattgat tatcaacagg aagaaaagaa accaaacgta acccatccaa
    56641 atccaccgga atacgtctgt tttctcccca ggtctgaaat tcaaaacccg actcggtatt
    56701 ggtcgcccag gccatcacca catttccgca accagccagt tgggtaattt gctgccagat
    56761 catctcccga atacgttttg atgtatcacc aacatacaca ccggcacgca cttccagtag
    56821 ccagattgcg agccgtccac gtaagcgcgg agggacattt tctgtaacaa ccacgaccat
    56881 actcatccgc cgcgcccccg gtgaccacta tcacccagcg tttcaggttc agggatggca
    56941 ggcggtaaca tatccggcgc gggttgtggt ggttcaattt cacctgcagc aaggactttc
    57001 tcaattaacg gtattaattt gcccgttaac ttagtgctac ggaaaatatc gcgacaggct
    57061 aatctgactt ctttatcagg ttctgcgggt tgcctcgctg ctatttcaaa tgcctttggc
    57121 acaaccgaat caaatttaat gatatcggct atgtcataaa caaatgaaag cggtttgcca
    57181 ctatgaataa atccaatagc gggcgcatat cccgcggcta atactgccgc ttcagaaata
    57241 ccgtacagac atgatgtggc agcactgatg cagcgattca caacatcgcc tttttcccag
    57301 tctttaggat cgtatttgcg accattccat ttcacaccat attgtttcgc cagtaatgca
    57361 taggtctggc gaacgcggga tccctcaatt ccccgtagct gatccactga acggcgagct
    57421 ggcggtggct cacgaaaacg taattcatac attttgcgca ccaccttcag gcgtagatct
    57481 tccgttaaag ccagctttgc ctggtagagt aatttatctg cccgcgcccc tccgggttgt
    57541 ccggaagagt aaacgcgaac gcccgcttca ccgacccaga ccagcagtgt tcccaccgtg
    57601 gcggccagat gcaccgccgc gtgggaaact ctcgttcccg gttcgagcat aatgcaggcg
    57661 accgatccca ccggaatgtg cgtgcggatc ccggttttgt cgatcagcac gaaagcgccg
    57721 tccagtacgt cgatttgacc gtactggagg aagatcatag aggtgcgatc ttttaacggg
    57781 atcggactca gtggtacaaa cgtcacacct ctgctccggg tttgatcagc atcagaccac
    57841 agccgaacgc gcggcttttg ccatacccct gactgagacg ttgcagaaat aacccgggat
    57901 cggtgaccgt aagcatcccc gtataatcca cgctactgaa ctggatcagt tgccgggagt
    57961 tttcccgccg cagttgctgt tgtcggtagg catcaaccga ggtatccagc agtgtaaaac
    58021 cgcttctctc cccctgcgct gccagccagt ccagcgccgc ctgttgttga tgcaaccaga
    58081 catcacttcc ttccgcctgc cccctcacct gccgtttcgc ctccatcagc agatcgtggc
    58141 gcttgcccgc tttacagatt gttggattcg cccgcaggtt gaaacaaagt tgttgtccgg
    58201 tacgcagctc gggcacaaat gaccggcatt cgatggtgaa cgtttcactt tccgccgggc
    58261 gttcctgcga cagtacaaaa aagcgaaacg cgccctggag ttcttcccga cgataaagaa
    58321 attgcctttc tttgccgcca gggaagagat cccacagcca ctgatgcatc acatattccc
    58381 cgcgatccac caaatgcagc aactgcgcag gcgaaagctg gccggtatgt aaggttattc
    58441 ttgagaggta catggttcct ccttgctgag ccacggcccc tgattgatgg tgcgttcccc
    58501 aaacagccac tgctgacgat ttaaaggaac atcccggcga cgtaatattt tgctcgcgac
    58561 caggccgtcg tgttcccctt cccaccagca ttcatcctga agtttcggga gtgagacttt
    58621 cagttcgcgg aaactatcct gatactgttg gtatgcgtta cgtaagacat cagacgcgtt
    58681 gccttcgagc agtaacggcg caagtggtaa cgccagcgga tgactttttc gccccagata
    58741 aagcggaaaa accggatgac gtaaaccgtc ctgcaactgt tcaaggctgt aaggcgcatc
    58801 gggggttgtt gccaccgcca ccatccacca ggcatcggtg tagtagtcgc gccgggagat
    58861 aatcgcgctc agaagatcag gggcgctcaa ctcttcgcga cggctgaaat aacgcgcttt
    58921 acgcacctct tttggcatct ggaccgtgtg ataatcccgt gcccagcgcg ggttacggct
    58981 ggcgcaaacc accagtgaat agtggcggtt aaacgcgttt aatcgttcgg tatcatcacg
    59041 ccgaatccct accccggcag ccagcagccc cagcaatgct gagcgcgaag gcagttcatg
    59101 ggtatgacgc acttcgccgg gggcatcgac gccccaggat gccattggcc catgaagctg
    59161 aaaaatcaaa tattggctca ttagccgccc cttacgcgca gataaagtcc agcacgtcct
    59221 tcatgcttcc ctgtttgttc atcacgtcaa agcttgcgca ttcggtcttc tgttcataga
    59281 ccgtattcat attttcgcga agcgttgtaa tacgctgcac cgccacatct aactgccggg
    59341 tgccattaat gggttcatag aaagccgccg ccagagaacg tggttgttcg gtgccttttt
    59401 ctgccagcgc ccaggaggcg taggcacggc tggcaaagct gttctgtttg ccggttgggg
    59461 agactttaag tgcggcttcc gtaaaggcgc gcaaggtctg attagctaac gcttcgtcac
    59521 cgccgaggtt ttcgaccagc agatctttat cgatgcagat ataggtgtag aacagcgcag
    59581 aaccgaatcc ggtttcacca agatgcccgg caccggcatc ttcagaagcc tggcgcaaat
    59641 catcaacggc ggtgaaaaaa tcatcttcga caatcgtttc actgacacca aatgcatgcg
    59701 cgacctggca ggcggcttca acattaaatt cgggtttatt cgccagcata cgaccaaaca
    59761 tagcgatatc tactgccatg cgatctttac gtaacaaagc gagatcttcc tcttttggcg
    59821 cgcgcttttc ttcggccagt tgatgggcca gcgcttttac ggcgtcaaat tctgccgggc
    59881 tgatatggac taattgttca gtttcggcgt tagtgagcgg atcttttggt tttttgtcgt
    59941 ttttagcttt cccaagataa tccgcaattt ttgccgccca ttcgatggct tttttctctt
    60001 cgatgccttt ctcaatcagg atagttgccg cctcacgcgc aatacgccca ctgcgaatac
    60061 caatatggcc cgccagtgcc tgttcaaaaa gtgcagaagt gcgccacgca cgtttcagac
    60121 tttgcgagga aacgcgcagt cgcgttgctc cacccaggac cacggttttc ggcgctccgg
    60181 tgtcatcacg gttaaggttg gctgcagggt aagcggttaa caaatgaagc tgaataaatg
    60241 tcgtcatgag atagtccttt attgattttg ttcgttatcg acgtcgccag cctggtaata
    60301 ttccagcgcc cagcggatac ggataaattc ggttggccgc tgatgacggc ggtgatgatt
    60361 gagcagatcg tcgctctcct ggcaccagcg gaagacaccc tctgccagag agtcaagatt
    60421 aacggaaccg tttaataatc tgactacgcg gcgtaactgg cgcagtaatt catccggtgt
    60481 ttttactgcc gacaggcgag taaaacgccc ctttgacatt accgccgcca gctgcgcagc
    60541 aaaaggcagc cgttcatcaa tggctttaac attagcgcta agtgcggcta tcagcgccag
    60601 tgcggtaata cgccattcag gttcatcctg ccactttatt tgtctgttct tcagaaacag
    60661 gcgaaatcca tccgtcagac aaacatcatt aaccgtcgta ctacgccgca gactggcacg
    60721 ttcgccgcgt ttctcctgca attcctcatg ccatttgcgc agcgtggctt tgtgctcctc
    60781 ttttacaata ctcattcagc agcctcctgc tttttctccc tggcggcttt agcactttgc
    60841 ttctccgccg atgttgtaaa atatttcttg cgcgcggtca tgacgcgttc caaatcaacg
    60901 ggctcataag gattggtgaa tacacgctcg tcaaaatcct gacgtgcgaa taaccaaatt
    60961 tccttttgcc atttgccgag taattcatcc gcatcctgac cttcttcaat ttggcgcact
    61021 aacctcagga agcgatgctg agttttgttc cagaagtcga tatccacaaa actgaaatca
    61081 ccccttgcac cttttggatc ggagaaccat gcttctttca atgcactccg taacagactc
    61141 agaatccgtg aagccgtttg cgcagccagc cgcagcttcg gtatctggcc ttctttttta
    61201 ttgagcagca gcgggaaatg gtgttcgtac caacagcgcg ctttcatgtt gtcgaaatca
    61261 taaccaaatc cccacaggcc cacttttgcc tgtttcagac tgctggcatt aaagagtttc
    61321 accaccagcg cgggaagttc cgtattgttt tctgacttac ccgtttcgat aaggcctaac
    61381 cagtcgcgcc agattaaacc gcccggttgt ggtttaacgg agtaaaactc accgccctct
    61441 ttaagtggta cacggtaagg cgttaaggga tgctgccaca tggcataatt cgcaccgtaa
    61501 tttttggtag tcatcaaact cagaagcgcg tcactctgct caccgcaaat atcgcagttg
    61561 ccgactgtcg tggtattaaa atcaatacga atacgccgcg gcattcccca gtacgcctgg
    61621 agtttattga cctgatcatc ggttaccacc gcaccggcca gttcgctggt acgcgtcggg
    61681 ccaagccagg ggaaaaccag atcgtcaaat tttttgggta gcggtaagtc ggcttcatcc
    61741 tgcggcatca cgttgagcca cagtttgcgc cacaaggggg cttgttgatt gccctgatac
    61801 tcctgcaatt caatcagagt cgtcatcggc ccaccgccgc gtaaaccggt gcgatagcct
    61861 ttgccacctg acggcgcatt taactgtagg gagaacagag ctaacgcaga acaatgagag
    61921 catacgtgtt cagtcacgcc acgcttaata aagtggtctt tattaaactt cgttgtttga
    61981 gcgccgggaa tctcaggcag tagcgaagcg acctgaactt tatcgcccat gagcacctcg
    62041 aaatcctgca taaatgaagg tgaatctggg ccaaactgga aagggtgttc taatgacagc
    62101 aatgcttccc gtagcttttc agcttccagc ccgtcttccc agatatcatc ccaacgacga
    62161 taatcttttg gcgcgaaact gctttgtagt aaccccagca aaaactgcca tgccgccccc
    62221 tggagatctg cccgcggcgc agcgatatcg acaacatttt catccgccag atcgactggc
    62281 gccagcttgc ctgttgttcc gtctttaaaa cgaacgggca accacggggt tgtcagaagt
    62341 gaaaacgagt tcatatgtat cctctcgtaa aaaagaccat ccctggtcgg ttgtccatca
    62401 ttccatcctt ttcggtgtat caactattca gagagtcctg tcgcaggtat ctcaatcaac
    62461 cttgccaatc aatccctccc tggccgaata cccgcaactt tcgtcatcag tgactaaaat
    62521 cacgtttgcc atttccggat cttgccgctg ttcaatgcac cactgcctga acgcttcccc
    62581 ttccagtaaa gaaaactcat cccgatgttt tttccaccag cttcgacgca ctctgacaac
    62641 gctcatttcc catgcgtgag caccggtggc ataaggcttc accacaccgg caatacaggt
    62701 agccagccac agggaaacag attcctcagc cagacgtgtc gacagctttt ccggaaggta
    62761 atcgttgata ttggcggcat agccaggctt gaagttcagg acaaactttt tagccattgc
    62821 gcgatcgcag taatatttgc ccacttgctc ctgctcgctg cgggcaaatc cttccggcat
    62881 taccacgtcc tcaccgtaga ctgattcaat aagaaggcgg gctgcgtgtg gcatttgaat
    62941 agcgccttgc tcacgcagta cacgctgcgt cagccagatt cgtccatgat cgggatagac
    63001 atatgcactg ttacgcatgg cactgccgaa ccattcgtca ccaggagcgt cgtcccagac
    63061 gggggccaga atcagcaatt caggagggga acgctcgtct tttccgtcac gctttaactg
    63121 accattaata tcgcggatat gccgctgtaa tcgccccgct cgctgaatca gcaaatcaac
    63181 aggggccagg tcggagatca tttcgtccag gtcacaatca acgctctgct ctaagacctg
    63241 agtacaaatg aggacttttc cggcacgctg tgaaccgtct tctttaccaa agcgtgccag
    63301 cgtctccatt tcaattcgct ggcgatcgct aaaagcaaag cggctatgaa agagtgaaag
    63361 gctggaagcg ggaatgacgc cgcgggcaag cagctgacga tgaaccttaa tagcgtcatc
    63421 gacagaattc cggatccagg cgatgcattt tccctgactt accgccgatt cgatacgcgc
    63481 aatactctct tgttcactat gaagccaacc cacgctgacg ctacgctcaa cgtctttgcg
    63541 cgtcgctacc cggtgtgagt tcacatcgga tttcgtgaca tgcgtcagcc aggggtaatc
    63601 atccttttca aggaacggag cttcttgctg gccctctgtg ccacgcgcaa aggcggcgac
    63661 gagtttgtcg cgctgctgtt gggataacgt agcagaaagc aaaatgacgc tgtttccgcc
    63721 acgcgcctgc cgctcgatca gcccttcaag aatgcacgac atgtaagcat cacaggcatg
    63781 gatctcatca gccagcagga ttttgttact caaccccaga agccgcagat tattatgttt
    63841 aaacggcatc actgccatca tcgcctgatc cagcgtgccg acgccaattt cagccagtag
    63901 cgccttcttg ttactgttgg caaaccaggc cgcacatccc tgactgaatg tttgttcatc
    63961 cggttcttct gacccgacta aatcaccgga ccagagtgat tcattgaagc ggtccattaa
    64021 tgtgcgggca ctgtgtgcca gcaccaagct ggggcgggac tctggcgaat agaaagcaag
    64081 ccaggttttg accagccgat cgtacatggc attggccgtt gccattgttg gcaggccaaa
    64141 aaacaaaccc tgtgctttcc tcgcagccat caacctgtgc gccaggataa gcgccgcttc
    64201 tgttttacct gcgccagtca cgtcttccag aataaataac tgtggccctg gctggctgat
    64261 atccagatcc agtacctttt gctgtaatgg tgtcgggtgc tcaataaaag gaaacagcgt
    64321 attaattccg gtgaaaggtg cggtttctgc ttttggagga aagacggtta aggcgttttg
    64381 agcctgaact aaagttttct gccagtaatc tttaatatcc attgggtgtg cgacgcgtgg
    64441 aaaaaatcgc gttgacgaac ccgtccagtc tgcgagtacg actgttgcag agatatacca
    64501 ggaaagttgt tttaaaagtt caacgccctc gtcatcatcc cagaatgtgg gaatctctat
    64561 gagcggaaac agtgccttga tttcaaggag aaaatctcgc gcggcagctt tgtcttcagg
    64621 cagaaaatta tccagctcat caatacggtc aggtggtcga ccatgatgcc cggtagttat
    64681 ggacatccac atctctatta cacgtgtaag tttacgagaa gagagtgaag atgaaggaag
    64741 caactcctca cattcactta aataataatt ccacagccag taacccagcg ttgaatgaga
    64801 gatcttttcg taattctttc tggaaccttc cggaatcttg agttcagggg ccaggtaaag
    64861 ttgctgaaaa gagcgggcaa attttccaat atcgtgccag cacagcaacc aagcgaaaaa
    64921 ttgagccgcc tgttccttgt cagaaatccc taattgacga aagtaatcag ccagcccgaa
    64981 gcaatttctt ttaaccatta aatagcccat tgcggccaca tccagcgaat gccagcaaag
    65041 aaggtgatag ccgtcgccac cctctttctc gccacgtcgg gtttttcccc agaaatcaaa
    65101 gaaagtca
//
LOCUS       DAZFBR010000013        63202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFBR010000013 DAZFBR010000000
VERSION     DAZFBR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.013"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(26..436)
                     /locus_tag="ACHJZ2_001009"
     CDS             complement(26..436)
                     /locus_tag="ACHJZ2_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4567168.1"
                     /translation="MPVTRYLCIFINVGLGEAAKRNVGTGENQIPDMASFASGDGWMK
                     LPNGKILQYGRGEAMPKLSTQTMRITFPIPFPKKADIAILTHSGDGGAPFGAGRGFVM
                     SVEGPTLTGFNSAYRTASTSDKVSMTYSWWAVGE"
     gene            467..1060
                     /locus_tag="ACHJZ2_001010"
     CDS             467..1060
                     /locus_tag="ACHJZ2_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4567169.1"
                     /translation="MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTASEWEQVGRLLAAGES
                     RQRVALIFDIGLSTLYKKFPSSATKNKLCHPLANRDK"
     gene            1120..2310
                     /locus_tag="ACHJZ2_001011"
     CDS             1120..2310
                     /locus_tag="ACHJZ2_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFO4567170.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNEAGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITSRYLASLVTS
                     VNSN"
     gene            2323..2841
                     /locus_tag="ACHJZ2_001012"
     CDS             2323..2841
                     /locus_tag="ACHJZ2_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO4567171.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            2898..3173
                     /locus_tag="ACHJZ2_001013"
     CDS             2898..3173
                     /locus_tag="ACHJZ2_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO4567172.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            3170..3325
                     /locus_tag="ACHJZ2_001014"
     CDS             3170..3325
                     /locus_tag="ACHJZ2_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO4567173.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            3318..5765
                     /locus_tag="ACHJZ2_001015"
     CDS             3318..5765
                     /locus_tag="ACHJZ2_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4567174.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRDTQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGQELKKARQEAAALAVQFKNTERPTNAQAKAMEAARK
                     NASELQAKYNSLRLSVQRQRQELSQAGINTRNLAHDERGLKNRITETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSTEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDAAAIQAA
                     TPVTLNMALANQRSMEENAQLLLGTKASFQLSNDDVSHVGDVLSATMNKSAADFQGLS
                     DALTYLGPVAKTAGVSLEQAAAMTGVLHDNNIRGSMAGTGSSAVVTRLQAPTGKAWDA
                     LKELGVKTSDKKGNMRPLFTILKEIQASFDKHKLGTSQKGEYLKTIFGEEALKSANVL
                     LAAAASGKLDKLTATLKASDGKTEELVKIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRNLTQTATKYVLKLDGWIQKNKSLASTIGLIVGGALALTGVIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHATTYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            5780..6259
                     /locus_tag="ACHJZ2_001016"
     CDS             5780..6259
                     /locus_tag="ACHJZ2_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4567175.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENMTLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            6259..7422
                     /locus_tag="ACHJZ2_001017"
     CDS             6259..7422
                     /locus_tag="ACHJZ2_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO4567176.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIATRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGRGVTASGKKIPQVIITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDEE"
     gene            7504..7722
                     /gene="ogrK"
                     /locus_tag="ACHJZ2_001018"
     CDS             7504..7722
                     /gene="ogrK"
                     /locus_tag="ACHJZ2_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFO4567177.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(7997..9358)
                     /gene="yegQ"
                     /locus_tag="ACHJZ2_001019"
     CDS             complement(7997..9358)
                     /gene="yegQ"
                     /locus_tag="ACHJZ2_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO4567178.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(9506..9838)
                     /gene="yegP"
                     /locus_tag="ACHJZ2_001020"
     CDS             complement(9506..9838)
                     /gene="yegP"
                     /locus_tag="ACHJZ2_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO4567179.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(10029..10751)
                     /gene="baeR"
                     /locus_tag="ACHJZ2_001021"
     CDS             complement(10029..10751)
                     /gene="baeR"
                     /locus_tag="ACHJZ2_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO4567180.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(10748..12151)
                     /gene="baeS"
                     /locus_tag="ACHJZ2_001022"
     CDS             complement(10748..12151)
                     /gene="baeS"
                     /locus_tag="ACHJZ2_001022"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO4567181.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(12148..13563)
                     /gene="mdtD"
                     /locus_tag="ACHJZ2_001023"
     CDS             complement(12148..13563)
                     /gene="mdtD"
                     /locus_tag="ACHJZ2_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4567182.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGSYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(13564..16641)
                     /gene="mdtC"
                     /locus_tag="ACHJZ2_001024"
     CDS             complement(13564..16641)
                     /gene="mdtC"
                     /locus_tag="ACHJZ2_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO4567183.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            complement(16642..19764)
                     /gene="mdtB"
                     /locus_tag="ACHJZ2_001025"
     CDS             complement(16642..19764)
                     /gene="mdtB"
                     /locus_tag="ACHJZ2_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO4567184.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(19764..21011)
                     /gene="mdtA"
                     /locus_tag="ACHJZ2_001026"
     CDS             complement(19764..21011)
                     /gene="mdtA"
                     /locus_tag="ACHJZ2_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO4567185.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            21291..21347
                     /locus_tag="ACHJZ2_001027"
     CDS             21291..21347
                     /locus_tag="ACHJZ2_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4567186.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            21619..21675
                     /locus_tag="ACHJZ2_001028"
     CDS             21619..21675
                     /locus_tag="ACHJZ2_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4567187.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            21947..22006
                     /gene="ibsA"
                     /locus_tag="ACHJZ2_001029"
     CDS             21947..22006
                     /gene="ibsA"
                     /locus_tag="ACHJZ2_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO4567188.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            22227..22886
                     /gene="yegL"
                     /locus_tag="ACHJZ2_001030"
     CDS             22227..22886
                     /gene="yegL"
                     /locus_tag="ACHJZ2_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4567189.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            22883..23644
                     /gene="pphC"
                     /locus_tag="ACHJZ2_001031"
     CDS             22883..23644
                     /gene="pphC"
                     /locus_tag="ACHJZ2_001031"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO4567190.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDVVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            23641..25581
                     /gene="yegI"
                     /locus_tag="ACHJZ2_001032"
     CDS             23641..25581
                     /gene="yegI"
                     /locus_tag="ACHJZ2_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO4567191.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(25594..26946)
                     /gene="yegD"
                     /locus_tag="ACHJZ2_001033"
     CDS             complement(25594..26946)
                     /gene="yegD"
                     /locus_tag="ACHJZ2_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4567192.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            27080..27928
                     /gene="alkA"
                     /locus_tag="ACHJZ2_001034"
     CDS             27080..27928
                     /gene="alkA"
                     /locus_tag="ACHJZ2_001034"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO4567193.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(28029..31347)
                     /gene="dgcE"
                     /locus_tag="ACHJZ2_001035"
                     /pseudo
     CDS             complement(28029..31347)
                     /gene="dgcE"
                     /locus_tag="ACHJZ2_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            31665..32306
                     /gene="udk"
                     /locus_tag="ACHJZ2_001036"
     CDS             31665..32306
                     /gene="udk"
                     /locus_tag="ACHJZ2_001036"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO4567194.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            32398..32979
                     /gene="dcd"
                     /locus_tag="ACHJZ2_001037"
     CDS             32398..32979
                     /gene="dcd"
                     /locus_tag="ACHJZ2_001037"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO4567195.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            33001..34854
                     /gene="asmA"
                     /locus_tag="ACHJZ2_001038"
     CDS             33001..34854
                     /gene="asmA"
                     /locus_tag="ACHJZ2_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO4567196.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(34907..35197)
                     /locus_tag="ACHJZ2_001039"
     CDS             complement(34907..35197)
                     /locus_tag="ACHJZ2_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4567197.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(35187..35525)
                     /locus_tag="ACHJZ2_001040"
     CDS             complement(35187..35525)
                     /locus_tag="ACHJZ2_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4567198.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            complement(35600..37183)
                     /gene="yegH"
                     /locus_tag="ACHJZ2_001041"
     CDS             complement(35600..37183)
                     /gene="yegH"
                     /locus_tag="ACHJZ2_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4567199.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNELEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(37384..37533)
                     /locus_tag="ACHJZ2_001042"
     CDS             complement(37384..37533)
                     /locus_tag="ACHJZ2_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567200.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            37842..38981
                     /gene="wza"
                     /locus_tag="ACHJZ2_001043"
     CDS             37842..38981
                     /gene="wza"
                     /locus_tag="ACHJZ2_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO4567201.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            38987..39430
                     /gene="wzb"
                     /locus_tag="ACHJZ2_001044"
     CDS             38987..39430
                     /gene="wzb"
                     /locus_tag="ACHJZ2_001044"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO4567202.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            39433..41595
                     /gene="wzc"
                     /locus_tag="ACHJZ2_001045"
     CDS             39433..41595
                     /gene="wzc"
                     /locus_tag="ACHJZ2_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO4567203.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            41688..42527
                     /gene="wcaA"
                     /locus_tag="ACHJZ2_001046"
     CDS             41688..42527
                     /gene="wcaA"
                     /locus_tag="ACHJZ2_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO4567204.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            42530..43018
                     /gene="wcaB"
                     /locus_tag="ACHJZ2_001047"
     CDS             42530..43018
                     /gene="wcaB"
                     /locus_tag="ACHJZ2_001047"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO4567205.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            43015..44232
                     /gene="wcaC"
                     /locus_tag="ACHJZ2_001048"
     CDS             43015..44232
                     /gene="wcaC"
                     /locus_tag="ACHJZ2_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO4567206.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            44207..45424
                     /gene="wcaD"
                     /locus_tag="ACHJZ2_001049"
     CDS             44207..45424
                     /gene="wcaD"
                     /locus_tag="ACHJZ2_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO4567207.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            45435..46181
                     /gene="wcaE"
                     /locus_tag="ACHJZ2_001050"
     CDS             45435..46181
                     /gene="wcaE"
                     /locus_tag="ACHJZ2_001050"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO4567208.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            46197..46745
                     /gene="wcaF"
                     /locus_tag="ACHJZ2_001051"
     CDS             46197..46745
                     /gene="wcaF"
                     /locus_tag="ACHJZ2_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO4567209.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            46774..47895
                     /gene="gmd"
                     /locus_tag="ACHJZ2_001052"
     CDS             46774..47895
                     /gene="gmd"
                     /locus_tag="ACHJZ2_001052"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO4567210.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            47898..48863
                     /gene="fcl"
                     /locus_tag="ACHJZ2_001053"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             47898..48863
                     /gene="fcl"
                     /locus_tag="ACHJZ2_001053"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO4567211.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            48866..49345
                     /gene="gmm"
                     /locus_tag="ACHJZ2_001054"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             48866..49345
                     /gene="gmm"
                     /locus_tag="ACHJZ2_001054"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO4567212.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            49342..50565
                     /gene="wcaI"
                     /locus_tag="ACHJZ2_001055"
     CDS             49342..50565
                     /gene="wcaI"
                     /locus_tag="ACHJZ2_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO4567213.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVISAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMEQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            50568..52004
                     /gene="cpsB"
                     /locus_tag="ACHJZ2_001056"
     CDS             50568..52004
                     /gene="cpsB"
                     /locus_tag="ACHJZ2_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO4567214.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            52197..53567
                     /gene="cpsG"
                     /locus_tag="ACHJZ2_001057"
                     /gene_synonym="manB"
     CDS             52197..53567
                     /gene="cpsG"
                     /locus_tag="ACHJZ2_001057"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO4567215.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            53622..55016
                     /gene="wcaJ"
                     /locus_tag="ACHJZ2_001058"
     CDS             53622..55016
                     /gene="wcaJ"
                     /locus_tag="ACHJZ2_001058"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO4567216.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            55018..56496
                     /gene="wzxC"
                     /locus_tag="ACHJZ2_001059"
     CDS             55018..56496
                     /gene="wzxC"
                     /locus_tag="ACHJZ2_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO4567217.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            56676..57956
                     /gene="wcaK"
                     /locus_tag="ACHJZ2_001060"
     CDS             56676..57956
                     /gene="wcaK"
                     /locus_tag="ACHJZ2_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO4567218.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            57953..59173
                     /gene="wcaL"
                     /locus_tag="ACHJZ2_001061"
     CDS             57953..59173
                     /gene="wcaL"
                     /locus_tag="ACHJZ2_001061"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO4567219.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            59184..60578
                     /gene="wcaM"
                     /locus_tag="ACHJZ2_001062"
     CDS             59184..60578
                     /gene="wcaM"
                     /locus_tag="ACHJZ2_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO4567220.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            60736..61731
                     /locus_tag="ACHJZ2_001063"
     CDS             60736..61731
                     /locus_tag="ACHJZ2_001063"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO4567221.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            61974..62867
                     /gene="galF"
                     /locus_tag="ACHJZ2_001064"
     CDS             61974..62867
                     /gene="galF"
                     /locus_tag="ACHJZ2_001064"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO4567222.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT        15379 a        16196 c        17098 g        14529 t
ORIGIN      
        1 ttcatataaa tcatcctgaa taaaattatt caccaacagc ccaccaactg taagtcattg
       61 acaccttgtc gctggttgat gcagttctgt aagcagaatt aaagccggtt aatgttgggc
      121 cttctacgga cataacgaat ccacgccctg caccaaaagg cgcaccgcca tcgccagaat
      181 gggtaagaat ggcgatatcc gcttttttag ggaaagggat aggaaatgta atcctcattg
      241 tttgcgtcga taatttcggc atagcctcac ctcgaccata ttgcaggatt ttcccgttgg
      301 gtaatttcat ccatccatca ccactggcaa acgaggccat gtccggtatc tgattttccc
      361 ctgtccccac attccgtttt gccgcttctc ccaaaccaac gtttatgaaa atgcagagat
      421 aacgggtaac tggcatcatc tccggttttt attcaggggg atgctcatgc ttattggcta
      481 tgtacgcgtg tcaacaaatg accagaacac ggaattgcag cgtaatgcgc tggagtgcgc
      541 aggatgtgag ctgatttttg aggacaaaat cagcggcacg aagtccgaca ggccgggact
      601 gaaaaaactg ctcaggacat tatcagcagg tgacactctg gtagtctgga agctggacag
      661 gctggggcgt agtatgcggc atctggtcat tctggttgag gaactgcgcg aacgcggcgt
      721 taattttcgc agcctgacag atgctattga taccagcacg ccgatggggc gttttttctt
      781 tcatgtgatg ggtgccctgg ctgaaatgga acgagaactc attgtcgagc ggactcgggc
      841 cgggctggaa gcggccagag ccaaaggtcg tattggtggc agacgaccga aactcaccgc
      901 gagtgagtgg gagcaggtcg gacggttgct ggctgcgggg gaatctcgtc aacgtgtggc
      961 gctgattttt gatattggcc tgtccacgct ctataaaaaa ttcccctcat cagcgacaaa
     1021 gaataaattg tgtcatccct tagccaaccg ggacaaatag cctgacatct ccggcacaac
     1081 tgaaaatacc actcacccat taaccacgga gttaaacgga tgagtgacta tcatcacggc
     1141 gtgcaggtgc tggagattaa cgacggcacc cgcgtcattt ccaccgtatc cactgccatt
     1201 gtcggcatgg tctgcacggc cagcgatgcg gatgcggaaa ccttccccct caataaaccg
     1261 gtgctgatta ccaatgtgca aagcgcaatt gcaaaggccg gtaaaaaagg cacgctggcg
     1321 gcgtcgttgc aggccatcgc tgaccagtca aaaccggtca ccgtagtcgt gcgtgtggaa
     1381 gacggcaccg gcgacgacga agaaacaaaa ctcgcgcaga ccgtttccaa tatcatcggt
     1441 accaccgacg aaaacggtca gtacaccgga ctgaaagccc tgctggcggc ggagtcggta
     1501 accggtgtta aaccgcgtat tctcggtgtg ccgggactgg acaccaaaga ggtggctgtt
     1561 gcactggcat cagtctgtca gaagctgcgc gctttcgggt atatcagcgc atggggctgt
     1621 aaaaccattt ccgaggtgaa agcctaccgc cagaatttca gccagcgtga gctgatggtc
     1681 atctggccgg atttcctcgc atgggatacg gtcaccagta ccaccgccac cgcgtatgcc
     1741 accgcccgtg cgctgggtct gcgcgctaaa atcgaccagg agcagggctg gcataaaacg
     1801 ctgtccaacg tcggggtgaa cggtgttacc ggcatcagcg catctgtatt ctgggatttg
     1861 caggagtccg gcaccgatgc tgacctgctg aacgaggcag gcgtcacaac gctgattcgc
     1921 cgcgacggtt tccgcttctg gggtaaccgt acctgctctg atgacccgct gttcctcttt
     1981 gaaaactaca cccgcaccgc gcaggtgctg gccgacacga tggctgaggc gcacatgtgg
     2041 gcggtggaca agcccatcac cgcaacgctg attcgcgaca tcgttgacgg catcaatgcc
     2101 aaattccgtg agctgaaaac aaacggctat atcgtggatg cgacctgctg gttcagcgaa
     2161 gaatccaacg atgcggaaac cctcaaggcc ggaaaactgt atatcgacta cgactatacc
     2221 ccggtgcctc ctctcgaaaa cctgaccctg cgccagcgta ttacttccag atacctggca
     2281 agtctggtta cctcggttaa cagcaattaa ggagcctgac cgatggcaat gccgcgcaaa
     2341 ctcaagttaa tgaacgtctt tctgaacggc tacagctatc agggcgttgc aaaatccgtc
     2401 acgctgccaa aactgacccg taagctcgaa aactatcgcg gtgcggggat gaacggcagc
     2461 gcaccggtag acctcggcct tgatgacgat gcgctgtcaa tggagtggtc gctcgggggc
     2521 ttcccggatt cggttatctg ggagctttac gccgcaaccg gtgtggatgc cgtgccgatt
     2581 cgttttgcag gctcttacca gcgcgacgat accggcgaaa cggtggccgt cgaggtggtc
     2641 atgcgtggcc gtcagaaaga aatcgacacc ggcgagggga aacagggaga agacaccgag
     2701 tcgaaaatct ccgtggtatg cacctatttc cggctgacga tggacggtaa ggagctggtc
     2761 gaaattgaca ccatcaacat gattgagaag gtgaacggcg tcgaccggct ggagcaacac
     2821 cgccgcaata tcggcctgtg attttcatcc ggtcagcccg gctgaccggt taacaccgat
     2881 tcagaagtga gaaaaccatg aacaaagaaa atgtgattac cctggacaat ccggtcaagc
     2941 gtggtgagca ggttatcgaa caggtcacgc tgatgaaacc cagtgcgggg acgctgcgcg
     3001 gtgtcagtct ggctgcggtc gcaaactccg aagtcgatgc actgattaag gtgctgccgc
     3061 gcatgaccgc accgatgctg actgagcagg aagtcgccgc gctggaactg cctgaccttg
     3121 tggcgctggc aggtaaggtg gtcggttttt tgtcgccgaa ctcggtgcag tgacgtttcc
     3181 gaaaaatctc tcggtcgatg acctgatggc ggatgtggca gtgatatttc actggccgcc
     3241 atcagaactg tatcccatga gcctgaccga actcatcaca tggcgcgaaa aggcgctccg
     3301 gcgaagcgga aacacgaatg agtaacaatg taaaattaca ggtattgctc agggctgttg
     3361 accaggcatc ccgcccgttt aaatccatcc gtacagcgag caagtcgctg tcgggggata
     3421 tccgggacac acaaaaatca ctgcgcgagc tgaacggtca ggcatcccgt attgagggat
     3481 ttcgcaagac cagtgcacag ctcgccgtga ctggtcagga actgaaaaaa gccagacagg
     3541 aagccgcagc tctggctgtc cagtttaaaa atactgaacg accgacaaat gcacaggcaa
     3601 aggcaatgga agccgcgcgt aaaaatgcgt ctgagttaca ggcgaaatat aacagcctga
     3661 gactgtcggt acagcgccag cgtcaggaat tgagtcaggc gggtattaat acccgtaatc
     3721 tggcgcatga tgaacgaggg ctgaaaaacc gtatcactga aaccaccgcc cagcttaacc
     3781 gtcagcgtga cgcgctggcg cgtgtcagtg cacaacaggc aaaacttaac gcagtcaaac
     3841 agcgttatca ggcaggaaag gaactggccg gaaatatggc ctcagtaggc gctgccggtg
     3901 tggggattgc ggcggcggga acgatggccg gagttaagct gctgatgccc ggttatgagt
     3961 ttgcgcagaa aaactcagaa ttacaggccg tgctcggtgt ggcaaaagac tccaccgaaa
     4021 tggccgcatt acgcaaacag gcgcgccagc tcggcgacaa taccgccgcc tcggcggatg
     4081 atgcggccgg tgcgcagatt attattgcga aagcgggtgg agatgctgcg gctattcagg
     4141 cggcaacgcc ggtcacgctg aatatggcac tggcgaatca gcggtcgatg gaagaaaacg
     4201 cgcaactgtt gctggggact aaggcatcgt ttcaactgtc aaatgatgat gtcagccatg
     4261 tgggcgacgt gttgtcggca acgatgaata agtcggcggc tgattttcag ggactcagtg
     4321 atgcactgac ttacctcggc ccggttgcga agacggcagg tgtaagtctt gaacaggcag
     4381 cagccatgac aggtgtgctg catgacaata acatcagggg gtcaatggcg gggacgggta
     4441 gcagcgccgt tgtcacccga ttacaggcac cgactggaaa agcatgggat gcactcaaag
     4501 agcttggcgt taaaacctcg gacaaaaagg gaaatatgcg tccgttgttc accattctga
     4561 aagagattca ggccagcttt gataaacaca agctgggaac gtctcagaag ggggaatacc
     4621 ttaaaaccat ttttggtgag gaagccctga aatcagcgaa cgttttactg gcagcggcag
     4681 caagcggaaa actggataag ctgaccgcca cgctgaaagc ctcggacggt aaaacggaag
     4741 agctggttaa aatcatgcag gataacctcg gcggtgactt taaggagttt cagtccgctt
     4801 atgaggcggt ggggactgac ctgtttgacc agcaggaagg cgcactgcgt aatctcacgc
     4861 agacggccac aaagtatgtg ttaaaactcg acggctggat ccagaaaaac aaatcactgg
     4921 cgtcaaccat cggcctcatt gtcggtggtg cactggcgct gactggtgtc atcggtgcca
     4981 ttggcctcgt agcctggccg gttatcaccg gcatcaatgc catcatcgcg gcagcaggcg
     5041 caatgggggc aatcttcacg acggttggta gtgccgttat gacggccatc ggggcgatta
     5101 gctggccggt tgtggccgtg gtggctgcaa ttgtcgccgg ggcgttgctt atccgtaaat
     5161 actgggagcc tgtcagcgca ttctttggcg gtgtggtgga agggctgaaa gcggcatttg
     5221 cgccggtggg agaactgttc acgccactta agccggtgtt tgactggctg ggcgaaaagt
     5281 tacaggccgc gtggcagtgg tttaaaaacc tgattgcccc ggtcaaagcc acacaggaca
     5341 ccctgaaccg ttgccgtgac acgggcgtca tgttcgggca ggcactggct gacgcgctga
     5401 tgctgccgct taatgcgttc aacaaactgc gcagcggtat tgactgggta ctggaaaaac
     5461 tcggggtcat caacaaagag tcagacacac ttgaccagac cgccgccaga actcatgcca
     5521 ccacgtatgg caccggtggt tatattccgg cgaccagctc ttatgcaggc tatcaggctt
     5581 atcagccggt cacggcaccg gctggccgct cttatgtgga ccagagtaaa aacgaatatc
     5641 acatcagcct gacgggtggt actgcgccgg ggacacagct tgaccgccag ttacaggatg
     5701 cgctcgaaaa atacgagcgg gataaacgtg cgcgcgcccg tgccagcatg atgcatgacg
     5761 gttaaggagg tgacgaaaaa tgatgctcgc gctaggtatg tttgttttta tgcgccagac
     5821 gctgccacac cagaccatgc agcgtgaatc agattatcgc tggccgtcaa attcccgtat
     5881 cggtaaacgg gatgcctacc agtttctcgg cgttggcgag gaaaacatga cgctggccgg
     5941 tgtgctttat cccgaactga ccggcggcaa gctgacgatg accacgctca ggctgatggc
     6001 agaggaaggt cgggcgtggc cgttgctgga tggcaccggc atgatttacg gcatgtatgt
     6061 catcagcagg gtgagtgaaa cagggagtat tttctttgca gacggcacac cccgaaaaat
     6121 tgattttacg ctgtcgctca cccgcgttga cgaatcactg gccgcgcttt atggcgatat
     6181 cggtaaacag gcggaatcgc tcatcggtaa ggctggcagt atggcgacta aattcacagg
     6241 tatgacgggg gcgggataat gctggatgca ctgacatttg atgcaggcag tacgctgacg
     6301 ccggattaca tgctgatgct cgacagcagg gatattaccg gcaatatcag cgaccgtctg
     6361 atgagcatga ctctgacgga taaccggggc tttgaggctg accagcttga tattgaactg
     6421 aacgatgccg acgggcaggt cggactgccg gttcgtggcg ctgtcctgac ggtgtatatc
     6481 ggctggaaag gttttgccct ggtatgcaaa gggaaattta ccgttgatga ggttgaacac
     6541 cggggcgcgc cggatgtggt caccatccgc gcccggagtg cagattttcg cgggacgctc
     6601 aattcccgcc gggaaggctc ctggcatgac accacgcttg gtgcgattgt tgaggcgata
     6661 gccacccgta acaggctgga agccagtgtc gctccgtcgc tggccggaat taaaatcccg
     6721 cacatcgacc agtcgcagga gtctgatgca aaattcctga cccgccttgc tgaacgcaac
     6781 ggcggtgagg tgtcggtaaa aatgggaaaa ctgttatttc ttaaggcggg gcggggggtg
     6841 acggccagcg gtaagaaaat cccgcaggtc atcatcaccc gcagcgacgg cgaccgtcat
     6901 cattttgcga ttgctgaccg tggagcctac accggtgtaa cggcaaaatg gctacacact
     6961 aaagacccga agcctcaaaa gcagaaggta aaactgaaac gcaaaaagaa agaaaaacac
     7021 ctgcgcgcac tggagcaccc caaagcgaaa ccggtcacgc agaagaaagc gccaaaagta
     7081 ccggaagcgc gcgaaggtga atacatggcc ggtgaggctg acaacgtttt tgccctgacc
     7141 acggtatatg ccacgaaagc gcaggccatg cgcgccgctc aggcgaagtg ggataagctg
     7201 caacggggcg ttgcggagtt ctccatcagc ctggctaccg gtcgtgcaga tatttacacg
     7261 gaaacgccgg tcaaagtgtc aggctttaag cgcgtcatag acgagcagga ctggacaatc
     7321 actaaggtga cacattttct gaataatagc ggcttcacga cgtccttgga gcttgaggtc
     7381 aggctttctg atgtggagta cgaaacagaa gatgaagaat gatgtgtttt atttatctgt
     7441 ttgttttgta aggataaatt aactaaaatg gcaccatcaa caaaaccgga agaggtgctc
     7501 gcgatgtttc attgtccttt atgccagcat gccgcacatg cgcgtacaag tcgctatatc
     7561 actgacacga caaaagagcg ttatcaccag tgtcagaacg tgaattgcag cgccacgttc
     7621 atcacttatg agtcggtaca gcgatacatc gtgaagccgg gagaagtcca cgccgtaagg
     7681 ccgcacccgt tgccgtcagg gcagcaaatt atgtggatgt aattaacaaa caggaagccc
     7741 ctcagtcgag gggctttttt tgtcgatgtg gtcaatgtgt ggacgtgacc agaaataaat
     7801 ccttttattt cattgtatta cgcgtaaaaa ataagcccgt gtaagggaga ttacacaggc
     7861 taaggaggtg gttcctggta cagctagcat tttatgggtt atgtttttca gcgaaacgga
     7921 tgataacctt aataaatgca gctgtatgtg atcggtttct aagaattttc catccgggaa
     7981 aaataatcga aattaatcac ttaccgtggg ggttacgcgt agtttccccg gagaaattac
     8041 gcatcagcag cgcgtaattt agctcaagat cctgcggcac cgggagccac acagtataac
     8101 catcgcctgg tgctaccggc atagcttcgc ctttggcgtt ttccatgtgc tcaagggtaa
     8161 agttaatgtt gccttgcggc gtcatcagct caaggctgtc gccaacggag aatttatttt
     8221 tcaccgctac cgccgcgagg tcccccttgc gctcaccggt aaactcacca acaaactgct
     8281 ggcggtcaga aactgaataa ccgtattcgt agttctgata atcgtcgtga gtatgacgac
     8341 gcaggaaacc ttcggtatag ccacgatgcg ccagaccttc cagagtttcc agcaggctgg
     8401 tatcgaacgg tttgcccgca gcggcatcat cgatagcttt acgataaacc tgtgcggtgc
     8461 gtgcgcaata gtagaaagat ttagtacggc cttcgatttt cagcgaatgc acgcccattt
     8521 tggtcaggcg ttctacatgg gcgatggcgc gcagatcttt cgagttcatg atgtaagtgc
     8581 cgtgctcatc ttcaaacgcg gtcatatact cgcccggacg ctgggcttct tcgatcataa
     8641 acactttgtc ggttggcgcg ccgataccca gcgtcggctc aacattttgc accggaatcg
     8701 gctcgtactt gtgtacgatg ttgccaacgt catcttcttt cccttcctgg acgttgtact
     8761 cccagcggca ggcgttggtg caggtaccct ggttcggatc gcgcttgttg atatagccag
     8821 agagcaggca gcgaccggag taggccatgc acagtgcgcc gtgaacgaag atctcgatct
     8881 ccatatccgg cacctgattg cggatctctt caatctcttc cagcgacagc tcgcgagaga
     8941 ggatcacgcg ggtcagtccc atttgctgcc agaatttcac cgtcgcccag ttcacggcgt
     9001 tagcctgtac cgagagatgg atcggcattt cagggaagtg ctcacgcacc agcataatca
     9061 gccctggatc ggacataatc agcgcatccg gccccatttc caccaccggt ttcaggtcac
     9121 ggataaaggt tttcagcttg gcgttgtgcg gtgcaatgtt gaccacgaca taaaactttt
     9181 tccccagcgc gtgggcttca ttgatgccga gctgaaggtt ttcgtggttg aattcgttgt
     9241 tgcgcacacg cagggagtaa cgcggctggc ccgcataaac agcatctgcg ccataagcga
     9301 aagcgtaacg catatttttc agcgttcccg ccggggaaag gagttccggt ttaaacataa
     9361 ttctctcgtt ctgatgacag gtcagatccg cttcacctga tgaagcggtt aggggagtgc
     9421 ccccacttta agggcgggca ttgtagcgct gtagggggga tctggtaaag cgccggacgc
     9481 caacaaccca cgcccggcat cgtaattacg tattgtcttt caccgtctgg cttgtgccat
     9541 tggctttaac ggaggcaatt ccggtttcac gagattgcgc ggtggcatac atctggctgg
     9601 agccgataat ttgatgattg gcggctttca gattgaaata gaatttgccg ttgcttgccg
     9661 tttttttctc atagcgttct tcttgcgggc tgttgctacg caccgacgcg atgccctttt
     9721 ccgcagaggc ttttgaggta tacagctcgc tggtgaggat cgtctcacca ttgcccgctt
     9781 ttagcacaaa ccggaactga ctatcactgc tcttactgag ttcaaaccaa ccagccataa
     9841 tgactccttg attgaaattg cgtatcgaaa acagcatcta aaggtgctgt ttaccgatta
     9901 aagtatgagc gattttctga tgcctgccag ggcggagaga taatgtcagt ttgctgacca
     9961 tgcgtgggat aatgttgccg gatctggtct gaatgccatc tccggctaac aaagtaatgt
    10021 cgctaaaact aaacgatgcg gcaggcgtcg gcttcccagc ggtaaccgac gccgtaaacg
    10081 gcgcggataa atgactgttc ggcgtcgaga gattccagct tgcggcgcag gtttttaatg
    10141 tggctgtcga tggtacggtc ggttactacg cggtagtcgt cataaagatg attgagcaat
    10201 tgctcgcggg agaacacttt tcctggttca tgagagagcg ttttcagcag acgaaattcc
    10261 gcaggcgtca ggtcaagcat tttaccgcgc catgaagcct gaaaacgacc ttcgtcgata
    10321 atcaacgggc tttcagcatc ctgttgctgc aactcacgct gcggtttgca acggcgtaaa
    10381 atggttttga cacgcgctac cacttcccgt gggctgtacg gcttgcaaat gtagtcatct
    10441 gcgccaatct ccagccctag caggcggtcg atctcttcga tttttgctgt caccatcacg
    10501 ataggaatgt cagaaaaacg acgaatttcc cggcacagcg tcaggccatc ggtgccaggg
    10561 agcatcagat ccaacaggat cagatccggc ggcgtctggc gcacgtacga cagtacctga
    10621 tcgccgtggc tgataagcgt cggcgcatag ctcgcagcac gcagataatc aatgagcaac
    10681 tgtcccagct tcggttcatc ttccacgatc aaaatacgcg gtgtgttttc gtcgattggt
    10741 aactcggtca tacttctctc tgtaaatccc gttccagcgg taactctact gtaatgctta
    10801 ccccgccaaa aggcgaatgg gcggcaataa tgcggccatt atgtgcttca acaatgttca
    10861 ggcaaatcgc cagccccagc ccggaaccgc cgctggcgcg gttgcgggaa ccttcggtgc
    10921 gataaaaacg ttcaaacaat ttttgtagct gatcgtcact gacacctggc gcactgtcgg
    10981 caaaggtcag gcgcaccgtt ttgtcgtgct gctcggcaga gattttcagg ctgccgccgc
    11041 tgtcagtgta gcgcaggctg ttttccagta agttattgaa taactgcatt aaacggtcgc
    11101 gatcgccaaa tacggtgata ctgtctggca gggaaaattg cagtttcagg ccacggctgg
    11161 caaatcgttc gcgaaatgcg ccgcctgcca cttccagcag tgggatcaaa tctaccggtg
    11221 ctttttgata ggcgagagcg ccttcatcag acatcgacaa ctgatgaaga tcgtcaacca
    11281 gtttggtcag tgtaccgacc tccgcctgta aagacgccac cgtctccggc gtgaatttgc
    11341 gcacgccatc ctgaatggct tccagttcac cgcgcagcac cgctaatggc gtacgcagtt
    11401 cgtgagaaat atcggccata aaatcgcgcc gcatttgctg gtttttctcc agcgtgctgg
    11461 cgagctggtt gaagtcttgc gccagtttgc ccagttcatc ttcactggtg ggcgttacgc
    11521 gggtagtgaa atcgcccgcc gccagtttgt gcgtgccatc gacaagtcgt tttaccggtg
    11581 ccagcaaacc gcgcgccagc agaaaagtag cgagtgccgc gagtaacgtt gccagggcga
    11641 caatcagcca gctggtttgc cgctgttgtt tatcgaaatt gatatcagta ttgcgggtca
    11701 gccgctcaac aggggaggca atcaccgcgc ccacttccgc accgttgacc agaatgggtc
    11761 gccgtgtacc gtcaggtgga atcggcgctc gcggaccaac cagcaccttg ttgttttgat
    11821 caaccaccca gaactgagta cgccagccgt gtggtggcat acccgggccg ggtttatctt
    11881 ccgaattatc gtgttcaaat gaacgcagga tctgaaagac aaagcgatca ttgttgcgca
    11941 ggaagcgcca gttgccatgc tgcgcatact gctcgccaag cgcatcactt aacagttgta
    12001 atcgctgttc attaccatgc ttgatgtaat caataaagcc acgctcaaaa ctgatacgca
    12061 ccgcccagtg catactgatc agcaagacaa tgcaggtggc gaaaatcgcc agaaacagtt
    12121 tgccggtaat accaggtcgc cagaacttca ttgcgcgctc ctttttcgcc gcgaaatagc
    12181 tacattttga tgcgtatcgt tcggcactct ggcaaagatg aacgccggaa gggcgatgat
    12241 aaacgccatg ctaagccagg tgtacataaa gacggtttgt gtggtgctgc tgtcgacgct
    12301 gacatgctgc gaaccaaaaa gtcccagcaa cagcccggcg atggtgacgc cgatactcat
    12361 cgacaattgc ataatcatcg acagcagact gttgccgctg ctcgccagat tgtccgggag
    12421 atctttcagc gtcagggtgt tcatggagga gaaacgggtc gagttgacca tcccttgtaa
    12481 aaacaggacg aacggcaaaa cgtagtacga gcccaacaga gcggtagtca taaacaacag
    12541 ggtgaccagc gacagaccca gcgtggtcgc taccagtacc cgacgataac caaagcgatt
    12601 caccacctgt acaacaatcc gcttcatccc catgctgcca agcaccatcg ggatcatcat
    12661 cagtccggca tgaaacggtg agaagccaag gccaatttgc aggaaaaccg gcgtcataaa
    12721 gggcaacatg ccgctaccga tacgtccggc aaagctcccc gccaggccca gcgaaaaggt
    12781 acgagtgcgg aacagtttca gactgaaaag agcgcggttg ttatttctgg cgtgcagcag
    12841 ataaagcacc agtgccacca caccaactgc gaccaggcca gtaatcgcca gcggcgataa
    12901 acctgtacct ttactgccgt ccagcgccag ggttaatacc gccatgccaa ccgccagcaa
    12961 taaaaagccg gaaagatcaa agcgccgcgt ctgcatggtg tagttcggca ttaacatcaa
    13021 tgtggcgatc gcaccgataa tccccaccgg aatgttgatc aaaaagatcc agtgccacga
    13081 cgcgtactcc accagcagac cgccgagcgc cggaccgagc agcggaccga cctgaccggg
    13141 taacgtgaca aaggtcatcg ccgccatata ttgctcgcgc ggtacgattt tcatcaccgt
    13201 caacctgccg accggcacca tcatcgcacc gccaacgccc tgtaacgcgc gtgccagcaa
    13261 cagttcgttc agcgtgccgg aaagcgcgca aaacagtgaa ccgagagtaa acagcacgat
    13321 agcggtaaag aaaatattgc gcacgccgac tttgtccgcc agccagccgc tggcgggcag
    13381 catcaccgcc acggtcagca cataagagac aatgaccatg tgcatatgca acggactttc
    13441 cccgaggctt tgcgccattg agggaagggc ggtgtttacg atggtggtgt ccagcgactg
    13501 cataaagaag ccgaaagcca caatccacaa ttgccagcgg gtgctgtcgg gaagatctgt
    13561 catttactcg gttaccgttt gtttagattt acgcgaaaaa cgcagccgca gacggtcgaa
    13621 aaagagatat accaccggcg tggtatacag cgtcaggagc tgactcatca ccagaccgcc
    13681 gacaatggtg atccccaagg gttgccgcag ctccgagccg tcaccgcccg acaacaccag
    13741 cggcagcgcg ccaaacagcg ccgccagggt agtcatcata atcgggcgaa aacgcagcag
    13801 acaggcctgg aaaatagctt cctgcggcgt caggttaccg tgccgttggg cttcaagcgc
    13861 aaaatcgacc atcataatgg cgtttttctt cacaatgccg attaatagca tgatccctat
    13921 cagggcgatt aggctgaacg gggcattgaa cagctccagc gccagcagcg ctccaacgcc
    13981 cgccgagggc agggtggaga gaatcgtcag cggatgtacg taactctcgt aaaggatacc
    14041 cagcacgata tacaccgtgg cgatggcggc gataatcagg atcacctgtg agttcatcgt
    14101 ctcctggaac acctgcgccg tgccggcaaa actgccgcgc accgtcgaag gcacaccaag
    14161 ctgggtcatt gcgcgatcga tcgccgcact ggcgtccgag agcgattttc cggtcggcag
    14221 gttgaacgag atggtcgagg ctgccgataa tccctgatga ttcaccgata gtggggcatt
    14281 cgccggttgc catttagcga aatacgacag cgggatcgct tttccttcgt tattgattac
    14341 gaacattttt tccagcgcgc tgatgtcctg ggtatagcgc ggatccactt ccatcaccac
    14401 tttatactgg ttcatcggct ggtaaatggt cgagatttgc cgctgaccga aggcgttatt
    14461 taacaggctg ttggcggcct gtacgtcgat tcccagccgt gccatggtgt cgcgatcgta
    14521 aaccagattc atctccgcgc cgttatcctg ctgatcggag ttcacgtccg ccagttccgg
    14581 caatgtcgcc agttttttgc ggattttcgg ctcccattcg cgcagtgccg ccaggtcgtc
    14641 ggataacaac gtgtactggt agctggcgtt cgactgacgt ccgccaacgc gaatatcctg
    14701 taccgccatc aggaacagat tcgcccccgg ttctttcgcc agcttcacgc gtagacggtc
    14761 aataatttgc tgcgctgttt cgctgcgttc gtcgcgtggc ttgagggtga taaacatcat
    14821 cccgctgttc actcgcgaac cgccggtaaa gccggtgaca ttatccactg ccggatcgtc
    14881 acggataatt ttcatgaaat cctgcaactt accgcgcatc gcctgaaacg aaatactctg
    14941 atccgcctgt atcccgccca tcaacacgcc agtgtcctgc tccgggaaga aggttttcgg
    15001 aatcgagata tatagccaga tattcaaagc aatggtgcca agcagcacca tgcccaccag
    15061 acgggtatga ttgagcaccc atttcagtga cttaccatag ccttgttgca gggctaccaa
    15121 catgcgacca aaaccacgca gtcgcttttg ctcgcgcggc ttgctggctt tcagcatcca
    15181 gccacacatc attggcgtta atgtcagaga aaccagcaac gaaataccaa tggcgacaga
    15241 aagcgtcacg gcaaactcgc gtaacagtcg gcccggcaat ccgcccatca acagcagcgg
    15301 caggaacacc gccaccagcg acagactcat cgacagcacc gtaaaaccga cttcgcgagt
    15361 accttgcagt gcggcttgca acggtttcat tcccgcttcc agatgacgtg caatgttttc
    15421 cagcaccacg atggcgtcat ccaccacgaa accagtagcg atggtgagcg ccattaacga
    15481 aaggttattg agactgaatc cgcacaggta catcgccgca aacgtaccaa tcagcgaaac
    15541 cggcaccgca acggcgggaa taatagtggc gcgacccgag cgcaggaata aaaagaccac
    15601 cagaatcacc agcgccaccg agataatcag cgtttgctcg acttcttcca gcgaggcgcg
    15661 aatggtgggg gagcgatcct gggcaatttg cagatcaatc gccgccggaa tggtttcctg
    15721 caactccggt aattttgccc ggatgctgtc taccgtctgg ataatattgg cttccggcaa
    15781 tttgcggatc atcagtaaaa tagccggttt ggcgttggtc atcccggcgt tgcgcacatc
    15841 ctgcactgag tcggtcaccg tcgccacatc gcccagacga accgcgccgc cgttgttgta
    15901 gtgaataatc agcggctgat attcagcggc ggttttcagt tcatcgttgg tctggatctg
    15961 ccagcggtga gtgccatctt ccagcgcccc ctgcggttta cgcacgttgg cattgctgat
    16021 ggcagtgcgt acgtcgtcca gcgacacgcc ctgattaaac agcgcctgcg ggttcagccc
    16081 gacgcgcacg gcgggcagcg agctgccgcc gacatcgaca tcaccgacac cgtcgatttg
    16141 cgaaatcgtc ggagccagct gcgtcgaggc gaaatcgtac agttcaccct gcgaataagt
    16201 atcggaggtt agcgtgagga tcataattgg cgcatccgac gggttcgctt tgcgataagt
    16261 cgggcggctg ggcatcccac tgggcagcag actttgtgcg gcgttgatcg ccgcctgcac
    16321 atcacgcgcc gcaccgttga tatcccggtc aaaatcaaac tgcaaaataa tacgcgtgct
    16381 gccgagcgaa ctgctggagg tcatttcact gaccccggca atgcgcccaa gtgagcgctc
    16441 cagcggcgtg gcaacggaag acgccattgt ctccggcgac gcaccgggca gcgaggcgct
    16501 gaccataatc accggaaaat cgacctgcgg cagcggggcg accggcagca tacggaaacc
    16561 cagtatgccg cacagggtaa tggcgaccga cagtaaaatc gtcgccaccg ggcggtaaat
    16621 gaagagggca aaaaacttca cttacgcctc ctcttcatgg cgagcaaagc gacttttggt
    16681 ccacaatgcc aggcggtcaa acagcagata aatcaccggc gtggtgaaca gcgtcagcac
    16741 ctggctgaca atcaggccgc cgaccatgcc gatacctaac ggacgacgta gttccgcgcc
    16801 gaccccggta ctcaacatca gcggcagcgc gccaagcaga gccgccaggg tggtcatcag
    16861 gatcggacga aaacgcaaca gacaagcctg gtagattgca tcgcgcggcg acatgccttg
    16921 ctcgcgctca gcagccagcg cgaagtcgat catcatgatg gcgttcttct tcacgatacc
    16981 gatcagcaaa ataatgccga taatcgcaat cacatccagt tcgctaccgg caatcattaa
    17041 cgccagcagg gcgccaacac ctgccgtggg tagcgtcgag agaatggtga tcgggtgaat
    17101 aaagctctcg tacagaatgc cgagcacgat atacatcgcc accaccgccg cgacaatcag
    17161 ccagacagtg ctgcccagcg ccgactggaa ggcgagggtg ctgccctgga attgcgtggt
    17221 gatatccacc ggcagattca gcgtcttctc ggtgtccata attgcctgca ccgcatcgcc
    17281 cagcgaatag ttatccggca cgttaaagga gatggtcgtt accgggaact gatccagatg
    17341 gttgatggaa agcggcgcaa aacgctgctc aattttggca attgagctta gcggcaccac
    17401 gccgccgtcg ctgctggtca ggcgaatggt atccagcgcc gcgaggcctg gggtattttc
    17461 ggtattgtgc tccagcacta cacgatactg gttagcctga gtataaatag tggaaatcag
    17521 tcgctgacca aacgcgttgt acagggcgtt atcgacatcc gccatgctga tccccagacg
    17581 gctggcgctg tcgcgatcaa cattgacata cgccaccagc cctttgtcct gccagtcgct
    17641 ggagacatca gaaatctgtg gcagctgctg gagtttttcc atcaactgtg gcacccaggt
    17701 actgagcgca tccagtgacg tggcctgcaa ggtaaactgg tactgggtgc ggctgacctg
    17761 agtatcgatg gtcaggtcct gcgttggttg caggaagaga tcgacgcccg gcactttatc
    17821 caccgccgtt tgcagacggg cgatgacttt ttgcacccga tcatcacgtt catccaacgg
    17881 tttgaggttg atctgcaaac gcgcactgtt cagcgacggg ttagtgccat caacgccaac
    17941 aaatgaggtc agactttgca ctgccggatc ctgcaaaatc acgtccgcga cctggcgttg
    18001 ccgctgggcc atattggcaa aggagctgga ttgcggtgcc tgcaaagtgc cctgaataat
    18061 gccgttatcc tgtaccggga agaaaccttt cggaatgaac acccacaata gaacgctaag
    18121 cagcagcgtg ctgagcgcca cgcttaaggt cagccacgga tgattcagca ctttcgccag
    18181 tccacgacca taggcggcga ttatcctgtc gaacattttt tccgaggcac gggagaagcg
    18241 gttctgttta cgcaacgact cctggctgag catccgtgcg cacatcatcg gtgtcagggt
    18301 tagcgacacc acggctgaga ttaaaatcgc taccgccagg gtaatagcaa attcgcggaa
    18361 cagtcgcccg acgatatcgc ccataaacag cagtgggatc aacaccgcaa tcagcgagaa
    18421 ggtcagcgag ataatggtaa agccgatttc acctgcgcct ttgagcgccg ccgccaacgg
    18481 tttttcgcct ttttcgatat agcgggaaat attttcgatc accacgatgg cgtcatcgac
    18541 cacgaacccg gtggcgatag ttaacgccat cagcgtcagg ttattgattg aaaaatcgag
    18601 aaacaccatg accgcgaagg tgccgattaa cgacagcggc actgcaacgc cgggaatgat
    18661 ggtcgccgga atattgcgca aaaacaggta gataatcatg actaccagcg cgatagccat
    18721 catcaattca aactgagtat catcgacgga tgcgcggata ttggtggtac ggtcagaaag
    18781 taccgtcacc ttcaccgatt tcggcagact ctcggtgagc tgtggcagca tctgccggat
    18841 gctgtcggcg gtggagataa tgttagcacc gggctggcgc tgaacattca tcacaatggc
    18901 ctgttcttta ttcgcccatg cgccgagcca gctgttttct gcaccttgct ctacagttgc
    18961 gacatcgccc agacgaatcg gcgcgccgtt ctggtaggcg atgattagct ggcgatactc
    19021 ttcggcggat tgcatctggt cgttagcgga aagcgtaacc gcacgggaag ggccgtcaag
    19081 gctaccttta gccgagttaa cgttagcgcc ggtaatggcg gtgcgcacgg tttcgctggt
    19141 caggccgagg gcggcaatcg cctgagcgtt aagtttgacg cgaacagccg gacgctggcc
    19201 gccggaaagc gtcaccaggc cgacgccgga aatctgcgag attttctgcg cgacgcgggt
    19261 ttccaccata tcttccactt gcgtcatcgg catggctgtt gaggtgacgg cgagcgtcat
    19321 gatcggcgga tctgccgggt tcactttgct gtaaaccggc gggttaggca gatcgctcgg
    19381 caacaagttg gtcgcagcgt taatcgcggc ctgcacttcc tgctcggcga catccagcgg
    19441 taatgttagc tggaactgta aagtgataac tgacgcaccg ccggaacttt gcgacgacat
    19501 ctgtttcagg ccagacatct gcccgaactg gcgttcgagc ggcgcggtaa cggcagaggt
    19561 catgacatcc gggctcgcac ctgggtagag cgtgaccacc tgaatggtcg gatagtccac
    19621 ttccggcagc gccgaaacgg gcagggcgcg ataaccgata atcccggcga gtaagatcgc
    19681 caccatcagc agcgtggtgg ccacaggacg cataataaac aggcgcgacg ggccgcctgt
    19741 gctgctcggg ggtaacactt gcatcaggag cgagctcctt ttttcgcgta ttcgcggctg
    19801 gtggctttct cttccggagt ggtggcgctc tgggcttcca ccacttccac tttcgcccct
    19861 tcggtcaggc gatcaatgcc gtctgtcacc acgcgatcgc ccgcagaaat acctgcgcgg
    19921 atcaccactt tctgactgtc ctgaatgccc ggcgtcacca gatgtttgct gaccttgttt
    19981 tcgctattca gcacccagac aaaatggcct tcattgccca tttgcagggc ggctgttggg
    20041 atcactacgg cgttttgttc ggtgtcgact aacatgcgcg cgttaacaaa ctgattggga
    20101 aacagcgcat catcctgatt attaaagcgt gctttcactt taatcgtacc ggtagtggca
    20161 tcgatttggt tatcgagact taacagcgtg ccttcactta atttcttcga gttggtgcga
    20221 tcccaggctt ctaccaccag cggttttccg gctttttgcg cctgcacgac ggtagcgata
    20281 tcgctttccg gcagggtaaa gagcaaatcg ataggatgcg tctgggtgat caccacgatc
    20341 ccggtggtat caccactgga gatttggtta ccaacatcaa cctgcttgag accaacgcga
    20401 ccatcgactg gtgcggtaat acggctccag tcgagttgca gctgcgcgct ggcgacgctt
    20461 gcttcatcag ccttaatggt gccttcggtt tcactgacca gcgcctgttg ggcatccagc
    20521 tcctggcggg agacgagatt ggtttttgcc agttgctgat aacgcgccag atcacggcgg
    20581 gcgttggtaa gcgtggcttt atcttttgcc agttggccct gggcttgtgc taatgcaact
    20641 ttgaactggc tggggtcaat ttctgccagt aaatcgccag ctttgacctg ctggccttcc
    20701 tggaaatgta acgccatcag ttgaccgtcc acgcggctac gcaccgtaac agtattagca
    20761 gcggtaatgg tgccaagccc ggtgaggtaa cgcggaactg cctgttctac ggcggtcgcc
    20821 gcctgaaccg gggctaatgg gccggaacgc ataccacggc gaccacccgc tggcgattgc
    20881 tgcgcttgtt tcgtcgcccc tggggctgca ctctgggagt cattgcggcc ttgccagaac
    20941 cagaatgcgg cgatggcggc gataaccacc acgattacga ttacccaacg ggatttataa
    21001 ctgcctttca tcgttaagag tttctcttcc tgaaacgttt cgcggaatga tactagtgta
    21061 gccagccaat aagggagaaa aatggaggaa ttatgaaaca ctatcgggaa tcgtctgaat
    21121 ggcgggcaca tttgcgagca cgcatccagt aataacacag gaaactattt tatctacgcg
    21181 ttagcgatag actgcttgca tggcgaaagg aggtaagccg acgatttcag cgggacgctg
    21241 aaacgggaaa gccccctccc gaggaagggg ccataaataa ggaaagggtc atgatgaaga
    21301 tactcatcat cgtggtgctc ttagtgatta gctacccagc atactaagac taccagggcg
    21361 ggggaaaccc cgctctaccc tcactcctga aagtatgcct tcacgttcag aatgtcaatc
    21421 cgcaggcttt gcagtctgcg atcctgccag caaatattct ttgcgagacg ttacgcaata
    21481 atcacagagg aaactatttt atccgcaagt cagcgataga ctgaattcag ggcgaaagga
    21541 ggtaagccga cgatttcagc gggacgctga aacgggaaag cccctcccga ggaaggggcc
    21601 ttaaataagg aaagggtcat gatgaagtgg atcatcatcg tggtgctctt agtcataagc
    21661 ttcccggctt actaagacta ccagggcggg ggaaaccccg ctctaccctc actcctgaaa
    21721 gtatgccttc acgataagat tgtcaatctg caggctttgc agtctgcgat cctgccagca
    21781 aatattcttt gcgagacgtt acgcaataat cacactggaa actattttat ccgcaagtca
    21841 gcgatagact gcatgcatgg cgaaaggagg taagccgacg atttcagcgg gatgctgaaa
    21901 cgggaaagcc cctcccgagg aaggggcctt aaataagaaa agggttatga tgaagcacgt
    21961 catcatactg gtgatactct tagtgattag cttccaggct tactaagaac accaggggga
    22021 gggggaaacc tttccctaac cctcactcct gaaattatgt cactccgccc ttagtgtcaa
    22081 tcgacggctc tccgttcttg cgaggcgaat agcaaaattt tctgcgccgc cgggcctaat
    22141 tctgaaagtt tcttcgtttg ctcttatttc attattgcaa tcggtatgat accggacctc
    22201 aataattctc taagaaagga tttcttatga gcgaacaaat cacgtttgcc accagcgatt
    22261 ttgccagtaa cccggaacca cgttgcccct gcattttgct gctggatgtt tctggttcga
    22321 tgaacggtag acccattaat gaacttaacg ccggattggt tacctttcgc gatgagctgc
    22381 ttgccgaccc gctggcctta aaaagagtgg aacttggtat tgtgacgttc ggcccggtgc
    22441 atgtggaaca accctttacc agcgccgcta attttttccc gcccatcctg tttgcccagg
    22501 gggatacacc aatgggtgcc gccattacca aagccctgga tatggtagag gagcggaaac
    22561 gtgagtatcg tgcaaatggt atttcgtatt atcgcccgtg gattttcctg atcaccgatg
    22621 gtgcaccaac cgatgagtgg caggctgccg ccaacaaagt gtttcagggg gaagaagata
    22681 agaagtttgc cttcttttcc atcggtgttc agggggcgga tattaagact ctggcacaaa
    22741 tcagcgttcg tcagcctttg ccactgcagg gattacaatt ccgtgaactg tttagctggt
    22801 tatccagttc gctgcgttcg gtttcccgct ccacgccggg aacggaagtt gtgctggaag
    22861 cgccaaaagg ctggacgtca gtgtgagctg gcgtctggtt tatgcatccg ctgttggaac
    22921 gtcgcacatt agcgctgatt taccttgtca ggatgcctgc cagatgcagg ttgcctggct
    22981 aaatgaccag cagccattac tggtgatgtt ccttgctgat ggcgcaggca gtgtctcgca
    23041 gggtggcgaa ggcgcgatgc ttgccatcaa tgaagcgatg gcttatgtgt cgcaaaaagt
    23101 gcagcacggt gaatttggac tcaatgatat tctcgccacg gatattgtgc tgaccgttcg
    23161 tcagcgactt tttgccgagg cggaagctaa agaattagca gttcgcgatt ttgcctgcac
    23221 gtttctggga ttgatttcat cggctaacgg caccttgatt atgcagattg gcgatggcgg
    23281 cgtggtggtg gattttggtc acggtctgca attaccgctt acgccgatgg tgggtgaata
    23341 tgccaatatg acgcatttca ttaccgatga agatgtcgtt tccagactgg aaactttcac
    23401 cagcactgag cgtgcgcata aagttgcagc atttacggat ggtattcagc gactggcgtt
    23461 aaatatgctg gataattctc cgcatgtgcc tttctttacc ccgtttttca atggactggc
    23521 ggcagcaacg caggaacaac tcgatttatt acctgaattg ttaaagcagt ttttgtcgag
    23581 cccagcggta aacgagcgta ccgatgatga taaaacgttg acactggcgc tgtggttacc
    23641 atgaaaccca ctttatatac tgctactggt gagtgcgtta cgccaggccg tgaactgggc
    23701 aaaggtggcg aaggcgcggt ttatgatatc aatgagtttg tcgatagcgt cgccaagatt
    23761 tatcacacgc cgccacccgc cttaaaacag gacaaacttg cctttatggc tgcgacagct
    23821 gacgcgcagt tgttgaatta tgtcgcctgg ccgcaggcaa cgcttcacgg tggacgaggc
    23881 ggaaaagtta tcggttttat gatgccaaaa gtttctggta aagaaccgat tcatatgatc
    23941 tatagcccgg cacatcgtcg ccagagttat cctcattgtg cgtgggattt tctactctat
    24001 gttgcgcgca atattgcttc atcttttgct acggttcacg agcacgggca cgtcgtgggg
    24061 gacgtaaacc agaacagctt tatggtaggt cgcgacagca aagtggtgtt gatcgatagt
    24121 gactcctttc agattaacgc caatggcaca ctgcatttat gcgaagtcgg cgtgtcgcat
    24181 tttacgccgc cagagctaca aaccttgcca tcatttgtcg gttttgaacg taccgcgaat
    24241 cacgataatt ttggccttgc gttgctgatt tttcacgtct tgtttggtgg gcggcatcct
    24301 tattctggtg tgccgcttat ctctgatgcg ggtaatgcgc tggagacgga tattgcccat
    24361 ttccgttatg cctacgcgtc agacaaccag cgacgtggtt taaaaccgcc gccacgatcg
    24421 attccgctgt cgatgttacc gggcgatgtt gaagccatgt ttcagcaggc attcacggaa
    24481 agtggcgtgg caaccgcgcg tccgacggca aaagcgtggg tagcggcact ggatttacta
    24541 cgccaacagt taaagaaatg taccgtttcg gcaatgcatg tttaccccgg tcatttgact
    24601 gactgcccgt ggtgtgcgct ggataatcaa ggcgttatct attttattga tctcggcgaa
    24661 gaggtcatta ccaccagtgg tgattttgtg ctggcgaaag tctgggcgat ggtgatggcg
    24721 tcagtagcac cgccagcatt gcaattgcca ttacccgatc atttccaacc gactggcagg
    24781 ccgcttcctt taggcctgtt acggcgcgaa tacatcattc tgattgagat cgcactgtca
    24841 gcgttatcgc tgttgctttg cggccttcag gcagaaccgc gttatattat tttggttcct
    24901 gtgctggcgg ctatctggat tattggcagt ctgacaagca aagtttataa agcagaaatc
    24961 cagcaacgcc gtgaggcatt taatcgcgcg aaaatggact atgaccattt agtcagccag
    25021 atccaacagt tgggcgggct ggaaggtttt atcgccaaac gggcgatgct cgaaaaaatg
    25081 aaggacgaaa ttctcgggtt accggaagag gagaaacgcg ctctggcagc acttcatgac
    25141 actgcaaggg aacggcagaa gcagaagttt ctggagggat tttttattga tgctgcctct
    25201 attcccggcg ttggccctgc gcgtaaagcg gcgttacggt cttttggtat tgaaacagcc
    25261 gcggatgtta cccgtcgtgg ggttaagcaa gttaaagggt ttggtgatca tctgacccag
    25321 gcggttatcg actggaaagc gagctgtgaa cgccgttttg tgttcaggcc gaacgaagcg
    25381 gtaacgcctg cagaaagaca agcggtaatg gcgaaaatgg ccgccaaacg acatcggctg
    25441 gaatcggcgt tgactgtcgg cgcgacagag ttgcagcgat tccgccttca tgctccagca
    25501 cggaccatgc cgttgatgga accgttacgt caggcggcag aaaaactggc tcaggcgcag
    25561 gcagatttaa gccgctgctg agccattttt caattaacga aacaccactt ccgcccagcg
    25621 tgccagcccg gcggtgacgg agccaaagtc atcgccgcct gcaatcggaa tgcccggcaa
    25681 ctgttctgcc agcgcttttt taatcagcgg agaacgggcg cttccgccgg tcaggtagat
    25741 aacgtccggt ttttcctggg cgttatccag cgccagttgc acctgttcct gaatccgcgc
    25801 cagtggctgg ctgagggcgc tttccagccc ttgctggcta atcagcgtag ccagttcatc
    25861 gctgataaac ggcagtgagg cgcgggtttc cgctacgctt gaaagcgcaa ttttgcactc
    25921 ttctgcgcta cgcaccaggc gatagcttaa acgctgacgc cagactttct gtagcagggc
    25981 cactttttcc ggttcgcggg catcgcgtac cagatcgtta agcagacgac cgttggcact
    26041 actgtagaaa tcactctgcg caggtacgtc gttgatggca accgcattcc accacggcag
    26101 aatcggcagg gcgatgcctt tttcggtttc gccacccata cccagcagtg gcatcaggtt
    26161 tttaaacgcc agtgcgatat ccagatcgtt accgccaata cggcaaccac tgtgacccag
    26221 caggctggct tcacgatcga gacgcgaacg ccactgcggc cccatcagca gcaatgaaca
    26281 gtcagtcgta ccaccgccga tatccaccac cagtacccgt ttttcttcct gtaaggtggc
    26341 ttcgtaatcc agcccagccg ctaccggctc gtactggaat accacgtccc tgaatccggc
    26401 acgcttcgcc gcgcgttcca gaatcccttg cgcctgggcg tttgcttcat cgccgcccag
    26461 cccctggaag ttgatcgggc gaccaatcac cgcctgagta atcgcttctg gcagctgcgc
    26521 ttgcgcctgc tggcgaatgt gcaacatcat tgcgcagacc agatcctcaa acagcgctac
    26581 ctgctgcggt tttaagccgc tggcaccgag gaacgatttt ggtgatttca caaaccacac
    26641 ttcttctgga tcatcaatgt actgtgccag tgaggaaaga ccgaactgca cgcttttcgc
    26701 cgtaacatcg atatcttctt cgcgattata acgaatcgcc cgacgcaaca gtgcctgcgt
    26761 ttcatcgtcg tctgccggaa catcatgatg gcggtacagc cattcgctta ccgcttcacg
    26821 cgttggcgcg caaagcattg aaggcagcag cgtgctgtcg ttttccattt ttagcaagtg
    26881 cggtttaccg tcacgcatga ccgctactga acagtttgct gtaccgtaat caaaaccaat
    26941 aaacacgaaa taatccccat gccggtgaag aaggggcgtg actttagcga aatgttgccg
    27001 tcgcgacaac cggaatatga aagcaaagcg cagcgtctga ataacgttta tgctgaaagc
    27061 ggatgaataa ggagatgcga tgtataccct gaactggcag ccgccgtatg actggtcgtg
    27121 gatgttggga tttctcgccg cccgtgcagt gagtggtgtg gaaacggtcg ctgacgatta
    27181 ttatgcccgt agtctggcgg tgggcgaata tcgcggcgtg gtgactgcta ttccggatat
    27241 agcccgccat actctgcaca taaatttaag tgcagattta gaacccgttg ccgctgagtg
    27301 tctggcgaaa atgagccgcc tgcttgatct gcaatgtaac ccgcagattg ttaacggtgc
    27361 gttgggcaag ttaggcgcgg cgcggcccgg attgcgttta cccggcagtg ttgatgcttt
    27421 tgagcaaggc gtgcgggcga ttttaggcca actggtgagc gtggcgatgg cggcaaaatt
    27481 gactgccaaa gtggtacagc tttatggcga acggctggat gattttccgg agtatatctg
    27541 cttccccacg cctcagcggc tggcagcagc agacccgcag gcattaaaag cgttaggtat
    27601 gccgttgaaa cgggcagagg cgctgattca tctggcaaat gcggcgctgg agggcacctt
    27661 accaatgaca ataccgggcg atgtggagca ggcgatgaaa acgctgcaaa cttttccggg
    27721 tatcgggcgc tggacggcga attattttgc tttgcgtggc tggcaggcga aagatgtttt
    27781 cctgccggat gattatctga ttaaacagcg atttccggga atgacaccgg cgcaaatccg
    27841 ccgttatgcc gagcgctgga agccctggcg ttcttatgcg ctgttgcata tctggtatac
    27901 ggaaggctgg caaccagacg aagcatgacg ttggtcgccg gatgcggtgc gagcgccttg
    27961 cggaatgagc gatttgtagg cctgataaga cgcgttagcg tcgcatcagg caatgggcaa
    28021 acgctccctc agttaatcgc gaaataacta ctattcatca gcaaatccag cggttgggca
    28081 tcggcaatca cctcaccata aatcagatcg acgccgatgc cagaaagcgt atccatcact
    28141 aagggtaaaa cgactggccc ggcgatggtt ttcatcccga gtcgctgagc gtgcccctga
    28201 ataatcgtaa tcagcatctc atccatcaaa ttaccctgca cgttggcgca taactcacca
    28261 tcaagtagca ggtaatctgc catattcgct ttcagcgagt tgaagatttg cagatcgcgg
    28321 cccacctgac tgaatacgat ccggcatccc cccagccgca gtttttgcac gctttctgcg
    28381 tgatcgaaaa tcgcttctgc cggaataatc agatgtaata accgtggtgg tagagggcta
    28441 ttttccagct gctcaagcag atcattcacc agcgtggcgc tactcaaacc ggcaacggaa
    28501 agggggaggg cgatgcttat gcctttactg gcaaccgctt ttgcggcctg ctggagaaat
    28561 tcgtggaata cccggcggtc aagagcatgg ctaagcgccg gatcgctgaa gctacgacga
    28621 aatgtttgtt catcaataat ctcgccttcg caactccaga gcttaagtga aatcagccac
    28681 aaattacgcg cttccgggat ccgtggcgaa gcgacaccgt gggcgagcat catcaactga
    28741 ttctctttaa tcatccgcca ctgttcatca agcgacatcg ccgcccgttc gctatgtgcg
    28801 gcagcttgct gcggttcgta aaccgtcacc cggccccggc caccattttt ggaggcataa
    28861 caggcgatat cagcctgcga catcacttct gccgcctgat gattgttgtc atcgatcaag
    28921 gtaatcccgg cgctggcacc tacccgatgt acccggcctt cccatataaa gtgatagtca
    28981 ttcacagcac tgataatacg tgtagcgata aaacgagcgc tttcaacatt gcagtctggc
    29041 agcagcagac caaattcatc accgccgagc cgcgccagca cgtcactgga gcgcagcata
    29101 ctcagcatca acgacgccag ttcgcgcagc aaagcgtcgc ccgccgcatg cccggcgctg
    29161 tcattcaccg ctttaaagcg atcaagatcg ataaacacca gtgcatgtcg ctgatgcgta
    29221 ctgtttaccg tttgcagcag gatacgcagt tgtttctcaa aactggcgcg gttggcgaga
    29281 tgcgtcagtg catcatggga ggcgctgtag ctcagctggc gcagcatttt gcgtgattcg
    29341 gtaacgtcct gaatcaccag taccgagcct atattgctgc cgtccagagt acttaacggc
    29401 gtaatactgt aatgcacgtc gtagctgccg ccgctccgac agtgcaacac cacatcttgt
    29461 tcgatcgcgg aacgtgaggt gtcggcactg taaatgttct ccattaatgg tccgttgtcg
    29521 ccaaaagtaa tatgcaacac cgtcaggagc ggaacaccta acgcttcttc ctgcgtccag
    29581 ccgctcatct tctccgccac tggattcata aaggtaattt tcatcgccat atcaatacag
    29641 accacggctt cgccgatgga gtccagcgta atgtgcaggc gctctttttc ctgaaacaat
    29701 gcctcgttaa gctgtttaac ctcagtcata tccatattaa tgccgagcag gcgttcgact
    29761 tcgccttctt tattcagtac ccggttggcg agggcgcgga tatggcggat gccatctttc
    29821 acggcaatgc gaaattccag tttaaagggc gagcgtgatt gcaacgaatc acgaatcact
    29881 ttttcggcgt gttggcgatc ttccggcagc acgcactcgt accacacctg ccagttcggt
    29941 ttgatatgcg gaggaatttc atacagctcg aacatccgct tatcccagct aaaaatattc
    30001 ggcttcagtt cccactccca gatgccaatc ccgcccgctt cgttagccag cgtgatgcgt
    30061 tccatcagtt gctgattcac ctgttcggtg cgttttagct cgttaatgtc ttcaatctga
    30121 gcgataaaat agagcggcgt gccatccgtg tggcgcacca gagagacggc aagcaacgcc
    30181 cagacaacat cgccattgcg gttgtagtaa cgtttttcca ttgaataggt gtttatttca
    30241 ccgcttatca gcttttcaac ctgttggaga tctttattga gatcctccgg ccaggtcagt
    30301 tgctgaaagg tgagtccgcg cagctcttcc tgactgtaac cgagaaactg gcagagtgct
    30361 ttgttggatt gcagccattg tccctcggtg cccactaatg ccatgccgat ggcggaatat
    30421 tccatggcgt tccgaaaacg ggtttcgctt tcggaaatgt gtttgcgttc cgcacgaaag
    30481 gcatacatca ccatcgtcat gatgttggcg ggcagcagga tcagcaaaaa cggtagccac
    30541 ggcatatggc tcatcaggta cgtacgcggc gtagcaagca gggagggatc cgcggccatc
    30601 atcagcgaca ccatcatcac cgtggtaagg aagatcaaaa aggcttccat tcgtggcagg
    30661 cgcacggcgc tccacatcaa cagcacaata atgaaagtaa aaggccacgg cagatacagc
    30721 atcgaaagcc agcttaacgt cagtgtgatg gctaacgtga gcagcgactc aaaaagcaac
    30781 cgtgggttgc gatggcgcag cagatagtgt ggtttaaata acaatcccag cggcaccagt
    30841 gccagagcgc cgatggattc tgacagtacc catatcaaaa atgccctgat gaggatcgtc
    30901 tccgggcgtc agcaggacaa ccagaacacc ccccaacaga ggtggaacaa tggcgctgcc
    30961 gagtgccaga cgcagccagt cagccagatt ttgcaagggg ttataccacg gcagcaattt
    31021 acgtagcagc actgccccga ccacggcttc aacaatattg atggtcgtgc aggtcatgtt
    31081 cagcgagctg gtggaaaaaa gcaggatgga tgcggcgata tttcccagcg agcagctcag
    31141 cgcaatccct ggccacatgc gcccggcatg gcgataaaac gccaccatca tgatggacgt
    31201 cgggaaccac agtggggcga gttgggtgcc aaactgcgaa agctcaagcg agaaaagggt
    31261 gaagataaac gagactaaac ctaaactgac caggtgaagc agcgggtggg gcagggcaat
    31321 taatacatgc tgtgattgtt tgctcattac cgctttatcc gcaaggccag gtcgccagta
    31381 ttcgtttctg tgtttatgct agtacaaaag ttttacattt tatatgcgaa ttgctcataa
    31441 agtgacgtaa aggcggataa tttgcgcaac tgcgtataac aatttttacc ttgtataaaa
    31501 ctgatcaacg taatttgcca ccgggaattc ttcgtcaaca attaactctg agcgcgcggc
    31561 aacgctattc gactggtatc aggcggatga aatccctata attgccgcgt ttggcgcttc
    31621 gtcgccccct tcctaacatc caggttaatc aggtcgctaa atatatgact gatcagtctc
    31681 atcagtgcgt cattatcggt atcgctggcg catcggcttc cggcaagagt cttattgcca
    31741 gtacccttta tcgtgaatta cgtgagcaag tcggtgatga acacatcggc gtaattcccg
    31801 aagactgcta ttacaaagat caaagccatc tgtcgatgga agaacgcgtt aagaccaact
    31861 acgaccatcc cagcgcgatg gatcacagcc tgctgcttga gcatttacaa gcgttgaaac
    31921 gcggctcggc aattgacctg ccggtttaca gctatgttga acatacgcgt atgaaagaaa
    31981 cggtgacggt tgagccgaag aaggtcatca ttctcgaagg cattttgttg ctgacggatg
    32041 cacgtttgcg tgacgaactt aacttctcca ttttcgttga taccccgctg gatatctgcc
    32101 tgatgcgccg catcaagcgt gacgttaacg agcgtgggcg ttcaatggat tccgtgatgg
    32161 cgcaatatca aaaaaccgtg cgcccgatgt tcctgcaatt cattgagcct tctaaacaat
    32221 atgcggacat tatcgtgccg cgcggcggga aaaaccgcat cgcgatcgat atattgaaag
    32281 cgaaaataag tcagttcttt gaataagctt gataaattgt gtaccgttca gtgataacct
    32341 ggtatgccct tgacgtaatt ggcgttaagg gagtgatgcg cgaaaggaga aaatgccatg
    32401 cgtctgtgtg accgagatat tgaagcctgg cttgatgaag gccgtttgtc gatcaaccca
    32461 cgtccgccag tggagcgtat taacggcgcg acggtggatg tacgcctggg caataaattt
    32521 cgtaccttcc gtggtcacac ggcagcgttt atcgatctga gcggtcccaa agatgaagtg
    32581 agcgccgcgc ttgaccgcgt gatgagcgat gagatcgttc tcgatgaaag cgaggcgttc
    32641 tatcttcacc caggagagct ggcgctggcg gtgacgctgg agtcggtgac gctgccagcc
    32701 gatctggtgg gctggctgga cgggcgttcc tcactggcgc gtctggggct gatggtgcac
    32761 gtcaccgcgc accgtatcga tccgggctgg tctggttgca ttgtgctgga gttctacaac
    32821 tccggtaagc tgccgctggc gctgcgtccg ggcatgttaa ttggtgcgct gagctttgag
    32881 ccgctttccg gcccggcggc gcgaccttac aaccgccgtg aagatgcgaa gtatcgcaac
    32941 cagcagggcg cggtagccag ccgaatcgat aaagactaat tcaggcccat tgaggatacc
    33001 atgagacgat ttctgacgac gctgatgata ctcctggtcg tgctggtggc cgggttatct
    33061 gcgttagtgt tgctggtgaa tccgaatgat ttccgcgact atatggtcaa gcaagttgct
    33121 gcacgtagcg gttatcaatt gcagctcgac gggccactgc gttggcacgt ctggccgcag
    33181 cttagtatcc tctccgggcg aatgtctctc accgcccagg gcgcgagcca gccactggtt
    33241 cgcgccgaca acatgcgcct ggacgtggcg cttttaccac tactgagtca tcaactgagc
    33301 gttaagcagg tgatgctaaa aggggcagtg atccaactga cgccgcagac ggaagcggtg
    33361 cgcagtgaag acgctccggt tgcaccgcgc gacaatacct tgccggatct gtcagacgat
    33421 cgcggatggt cgtttgatat atccagtctt aaggtggcgg acagcgtgct ggtgttccag
    33481 catgaagatg acgagcaggt gacaatccgc aatatccgcc tgcaaatgga acaagatccc
    33541 caacatcgtg gctcatttga gttctccggg cgggttaatc gcgatcagcg cgatctcacg
    33601 atatccctta acggtacggt agatgcttct gattatccgc atgatttaac ggcggctatt
    33661 gaacaaatta actggcagtt gcagggtgcc gatttaccaa aacaaggtat tcaggggcag
    33721 gggagtttcc aggcccagtg gcaggagtca cgtaaacgcc tttcatttaa ccaaattagt
    33781 ttgaccgcca atgatagtac gctgagcggg caagcacagg tcacgctgac agagaaaccg
    33841 gaatggcagc tgaggctgca attcccgcaa ctgaatcttg acaacctcat cccgcttaat
    33901 gaaaccgcga atggtgaaaa cggtgccgcg cagcaggggc agagccaatc aacgttgccg
    33961 cgcccggtta tttcttcgcg tattgatgaa ccggcctatc agggactgca aggctttacg
    34021 gctgatattt tgttgcaggc cagtaacgtg cgctggcgcg gaatgaattt tacagatgtt
    34081 gccacgcaaa tgaccaacaa gtcgggtttg ctggaaatta ctcaactaca gggcaagctt
    34141 aacggtggac aggtttcact gccgggcacg ctggacgcga catcaataaa tccgcggata
    34201 aacttccagc cacggctgga aaacgttgag attggcacca ttctgaaggc gtttaactat
    34261 ccgatttcgt tgaccggaaa aatgtcattg gctggtgatt tctccggtgc tgacatagat
    34321 gccgacgcat tccgccacaa ctggcaagga caggcacatg tcgaaatggc cgatacgcgc
    34381 atggaaggga tgaacttcca gcagatgatt cagcaagcgg tagaacgtaa tggtggtgat
    34441 gtgaaggccg ctgaaaactt cgataacgta acgcgtcttg accgctttac caccgatttg
    34501 acgttgaagg atggcgtcgt gacgttaaac gacatgcaag gtcaatcgcc tgtgctggcg
    34561 cttacagggg aaggcatgtt gaatctggca gatcaaacct gcgacaccca gtttgatatt
    34621 cgggtcgtgg gtggctggaa cggggaaagc aaactgattg atttcctcaa agaaacgcca
    34681 gtaccgctgc gggtttatgg caactggcag caactcaatt acagtctgca agtggatcag
    34741 ttactgcgca aacatctaca ggacgaagcg aaacgtcgcc tgaatgactg ggccgagcgg
    34801 aataaagatt cccgcaatgg caaagatgtg aagaagttgc tggagaagat gtaagacaag
    34861 ttatatagat ggcggcatca atttgtcgcc atctgtattt atagcactaa gcagcatgtg
    34921 gatattgaac ttcaatacgt tgaatatgta gtcctgcatg agccatcatg gtgatcaatt
    34981 tatcgatact aaagatctgg attttacctc tggcaagttc agagatgcga ggttgagtga
    35041 cccctaaagc agaagctgcc tgagcctggc taaacccttg ttgttcaatc cagttattaa
    35101 tgatcgttac aagctcggca cggatccgca tattttccgc ttgttcaggg gtgtcactca
    35161 ccgcatccca cacactatca aaggtttcaa ttttcatttt tccgctcctg aacaagttct
    35221 ttatagcgtc gtttggctaa atcaatatcc gattgactgg tagtctgtgt tttcttttga
    35281 aaacaatgca atacgtaaac cgcttcttca aattttgcga catacatgac gcgatagatc
    35341 ccatcagcat cgcgagtacg aatctctcgc acaccagggc cgatagttga aaaaggtttc
    35401 cagtcgtatg ggtttaaccc ttgttggatc ctgtcgagtt gatagccaat ttcctgtcga
    35461 gacgattctg gaaatgcgcg taaatcgtca agactggtat caagaaaagc cagctttttt
    35521 ctcattactc catccttaga ttatactaaa ttttgtataa atcaaatgcg cagtttgaac
    35581 ctgcgcattt gatgttttat cacacctcgt actccatctc gccatcctta cgcagcggta
    35641 taatctgcac cttctgcacg cgatggcttt ccacctgcaa cgttttaagc aaataatccc
    35701 ccacctgaac ttcttcgcca ggttttggaa tacgctgcaa atattccatc aatagcccgg
    35761 caatggtgtg atattcacgt ttttcatcca gcggcagcgg cacatattgc accagatctt
    35821 ccagcggcat atgaccattc gccgtccagg aaccgtccgc attcttctga atatcatggc
    35881 gggcgtcgat ctcttccaat tcgttcggta agttaccggc aatggtttca gtgacgtcac
    35941 ttaatgtcac aatcccttcc accgagccaa actcatccac cacaaaagca aagtgtgtgc
    36001 gggcattacg gaactgctcc agggcaggta acaacggcaa ggtttccggg aacaccagcg
    36061 gctggcgaat taacacccgc aggttgagcg gttcgccgcg cagtgattgt tgtaacaggt
    36121 cgataacgtg aacaacaccg agcaaatctt ccgcgtcatc gccatcagta accaccagcc
    36181 gcgtatgctg atttcgctcc agcaactggc ggatctcgtc ttccggcgcg ttgagatcga
    36241 tatgctcaat atcgtggcgc gacgtcataa tgctgctgac ggtacgctgg ttaagattaa
    36301 gtacccgctc aatcatccgc cgttcctgcg gattaaagat ctgctggtta ccatgatcca
    36361 ccagcatgga cgcggtttcg gcgtccagtt ccgcatcctc tttttgcccg ctaagcaggc
    36421 gcattaccgc ttcggtagtc cgctggcgca gtgtctggtt agcggaaaga aaacgtcgcc
    36481 ggttaaagat agccaactga ttgagcgcct cgatcatcac cgagaaacca atggcagcgt
    36541 acaggtagcc tttcggaatg acgaagccga aaccttctgc caccaggcta aagccaatca
    36601 tcaacaggaa gcttaagcag agaataacga tggtcgggtg actgttaaca aattgcgtta
    36661 acggcttgct ggccatcaac atcaggctga tagcgataac cacggcggcc atcatgacca
    36721 gtaaatggtc gaccatcccg acggcggtaa tcactgagtc gagtgagaag atggcgtcca
    36781 gtaccacaat ttgtgtcacc accccccaga acttcgcacc tttgcgttgt gtgggattat
    36841 tgctgtcttt cccttccagc cgttcgttca gctccatcgt ggctttgaac aacaggaaga
    36901 aaccaccaaa cagcatgatt aagtcgcggg cgctaaaggt aaacgagcgg aagctgaaca
    36961 gcggttgagt cagggtgacc agccaggaga ttgacgccag cagtaacagg cgcattaaca
    37021 tcgccagcaa cagcccggta acccgtgcgc ggtcgcgctg cttcggcggt agtttttcgg
    37081 cgaggatggc aataaagacc aggttatcaa tgccgaggac cagttcgatc acaatcagcg
    37141 tgattaaccc ggcccagata gacggatcgg caatccattc catagtaagt acaaaaccta
    37201 ttcgttgtat gacaaatgtc acaattcgat catggataaa tcggggcaaa aatgcaatgt
    37261 tgcagcacta tttatctcat attatttgag ggtaataacc tcacattatc cctgaattaa
    37321 aagtggtaat aataaaacat tatttaagaa atatcgcatg tggagacgca atattaaata
    37381 ttgtcaacct aaagaaactc ctaaaaacca tattgaatga cacttaatat aattcttaaa
    37441 aatagccaat tgccgaattg ttaccttgcc tgctattccg ttagctgtaa cacttcctcc
    37501 tgcattattg gagagccaat attcaattac cataatgtca ccttatattc ggaagttaat
    37561 ttccgcaata accctttctc atcaacgatt gtatgcagag agggtttgaa aaattaagct
    37621 aaatcagtgt attggtagct aaaaagccag gggcggtagc gtgtctggat gcctgaaaga
    37681 ccagtctgaa ttattctgtc aatagcctgc ggatgaaatc aattttttta ggactgatgc
    37741 cagttaaatt tttattcaat taactgcaag gtaaatgtgc atggcgacat tgctattaat
    37801 agtgcacagg ataattactc tgccaaagtg ataaataaac aatgatgaaa tccaaaatga
    37861 aattgatgcc attattggtg tcagtaacct tgataagcgg ttgcacagta cttccgggca
    37921 gcaatatgtc gacgatgggc aaagacgtca tcaaacagca ggacgctgat ttcgatctcg
    37981 acaaaatggt gaatgtttat ccgctgaccc cgcgcctgat tgaccaatta cgcccacgcc
    38041 cgaatgtagc gcgccccaat atgacgctgg aaagtgagat cgcgaattac cagtatcgcg
    38101 tcgggccggg ggacgttctt aatgtcaccg tctgggatca cccggaactc accacgccag
    38161 ccggtcagta ccgcagctcc agcgacaccg gcaactgggt acagcctgac ggcactatgt
    38221 tttacccgta tatcggcaag gttcacgtag tcgggaaaac gctcgctgaa atccgcagtg
    38281 atattaccgg gcgcttagcg acgtacatcg ctgacccgca ggtggacgtt aatatcgccg
    38341 ccttccgctc acaaaaggcc tatatctcag gtcaggtgaa taaatctggt caacaggcga
    38401 tcaccaacgt accactgacc attctcgacg ccatcaacgc cgcaggtggc ctgaccgaca
    38461 ccgctgactg gcgcaacgtg gtgctaacac acaatggtcg tgaagagcga atttctttgc
    38521 aggcgctgat gcaaaacggc gacctcaatc agaaccgact gctttacccc ggcgatattc
    38581 tctacgtgcc gcgtaatgat gatctgaaag tgtttgtgat gggtgaagtg aagaaacaga
    38641 gcaccctgaa aatggacttt agcggcatga ccctgaccga agccctgggc aatgctgaag
    38701 gcatcgacat gaccacctcc aacgccagcg gcatctttgt cattcgtccg ctgaaaggcg
    38761 agggcgggcg taacggcaaa attgccaata tctaccagct ggatatgtcc gatgccacgt
    38821 cgctggtgat ggcgacagaa ttccgcctgc aaccttatga cgtggtgtat gtcaccaccg
    38881 ccccggtttc ccgctggaac cgtctgatca atcagttgct gccaactatt agcggtgtcc
    38941 gttacatgac ggatacagcc agcgacattc ataactggta atcgtcatgt ttaacaacat
    39001 cttagttgtc tgtgtcggca atatttgccg ttccccgacg gcggaacgct tactgcaacg
    39061 ttatcacccg gagctgaaag tggagtccgc tggactcggc gcgctggtcg gtaagggcgc
    39121 tgatcctacc gctatcagcg tcgccgcaga acatcaactg tctctggaag gccactgtgc
    39181 acgacaaatc agccgccgcc tgtgtcgcaa ctatgacctg attttgacca tggaaaagcg
    39241 ccatatcgaa cgcttatgcg agatggcacc ggagatgcgc ggcaaagtga tgctgtttgg
    39301 tcactgggat aacgaatgtg aaatccccga tccgtatcgc aaaagccggg aaacgtttgc
    39361 agcggtgtac acattacttg aacggtctgc ccgccagtgg gcgcaggcat tgaacgcaga
    39421 gcaggtataa gaatgacaga aaaagtaaaa caacatgccg ctccggtaac gggcagtgat
    39481 gaaatcgata ttggtcgcct ggtcggcacc gtcattgaag cgcgctggtg ggtgattggc
    39541 atcaccgctg tattcgccct ttgtgccgtg gtttacacct tcttcgccac gccgatttat
    39601 agtgccgacg cactggtaca aatcgagcaa agcagcggca attcgttagt gcaggatatc
    39661 ggatcggcgt tagccaacaa accgcctgca tcggacgccg agatccagtt gattcgttcg
    39721 cgcctggtgc ttggtaaaac ggtggatgat ctcgacctcg atattgcggt gagcaaaaac
    39781 acgttcccga ttttcggtgc gggctgggat cgcctgatgg gacgccagaa cgagacggtg
    39841 aaagtgacta cctttaaccg tccgaaagag atggaggatc aggtgtttac gcttaatgtg
    39901 ctggacaaca aaaactacac cctgagcagc gatggcggct ttagcgcccg tgggcaagcg
    39961 ggccagatac tgaaaaaaga aggcgtcacg ctgatggttg aagccattca cgcccgcccg
    40021 ggcagtgagt ttaccgtcac caaatactcc acgctgggga tgatcaatca actgcaaaac
    40081 agcctgacgg taacggagaa cggcaaagac gcaggcgtac tgagcctgac ttataccggt
    40141 gaagatcgcg aacagatccg cgacattctt aacagcatcg cccgtaacta tcaggaacaa
    40201 aatattgagc gcaaatcggc ggaagcgtcg aaaagcctcg ctttcctcgc gcaacagtta
    40261 ccggaagtac gtagccgcct tgatgttgcc gaaaacaaac tgaatgcctt ccgtcaggat
    40321 aaagattctg ttgatctgcc gctggaagcg aaagcggtgc tcgattcgat ggtgaacatc
    40381 gacgcccagt tgaacgaact gacctttaaa gaggcggaaa tctccaagct gtacaccaaa
    40441 gttcaccccg cgtaccgcac gctgctggag aaacgtcagg cgctggaaga cgaaaaagcc
    40501 aaacttaatg gtcgcgtaac ggcgatgccg aaaacccagc aggaaattgt ccgtctgacc
    40561 cgcgatgtcg agtctggtca gcaggtctat atgcaactgc tgaataaaga gcaggagctg
    40621 aaaatcaccg aggccagcac cgtcggcgat gtgcgcattg ttgacccggc aatcactcag
    40681 cctggtgtgc taaaaccgaa gaaagggctg attatccttg gggcgattat ccttggcctg
    40741 atgctctcta tcgtgggggt gctgctgcgc tcgttgttta atcgcggcat cgaaagcccg
    40801 caggtgctgg aagaacacgg tatcagcgtc tatgccagca tcccgctgtc ggagtggcag
    40861 aaagcgcgcg atagcgtcaa aaccatcaaa gggattaaac gctataaaca gagccagcta
    40921 ctggcggtgg ggaatccaac cgatctggcg attgaagcca tccgcagcct tcgtaccagt
    40981 ttgcacttcg cgatgatgca ggcgcagaac aatgtgttga tgatgaccgg ggttagcccg
    41041 tcaatcggta aaacctttgt ctgcgccaac ctggcggcgg tgatcagcca gaccaataaa
    41101 cgcgtgttgt tgatcgactg cgatatgcgc aaaggctaca cccatgagct gttgggcact
    41161 aataacgtta atggcctgtc ggaaattctg attggtcagg gcgatattac tacagctgct
    41221 aaaccgacct ctattgccaa atttgacctg atcccgcgcg gtcaggtacc gccaaatcct
    41281 tctgaactgt tgatgagcga acgctttgcc gaactggtga actgggcgag taaaaactac
    41341 gacctggtgt tgattgatac gccgccgatt ctggcagtga ccgatgcggc aattgttggt
    41401 cgtcatgtcg gaaccacgtt aatggtggcg cgttatgcgg tcaacacatt gaaagaagtg
    41461 gaaaccagtc tgagccgctt tgagcaaaac ggtattccgg tgaaaggggt gattctgaac
    41521 tccatcttcc gccgcgccag cgcgtatcag gattatggct attacgaata cgaatataag
    41581 tcggatgcga aataacgagg cctgcattcg caccgccccg taggccggat aaggcgctca
    41641 cgccgcatcc ggcaagcaaa ccagctcata agccgggagt acaacctatg aaagacaatc
    41701 cgctgatctc aatctatatg ccgacctgga accgccaaca actggcgatt cgggcgataa
    41761 aatcggtctt gcgccaggac tacagcaact gggagatgat catcgtggat gattgctcca
    41821 cctcctggga acaactgcaa cagtacgtca ccgccctcaa cgatccgcgt attacctaca
    41881 ttcacaacga cattaacagc ggggcgtgcg cggtacgtaa ccaggcgatt atgctggcgc
    41941 aaggggaata cattaccggg atcgatgacg atgatgaatg gacgcccaac cgcctgagcg
    42001 tcttcctcgc ccataaacag caactggtca ctcacgcctt tttgtacgct aacgattacg
    42061 tttgccaggg cgaagtttat tcccaaccgg caagcttacc actgtatccc aaatcgccgt
    42121 actcacgccg cctgttctat aaacgcaata tcattggcaa tcaggtcttt acctgggcat
    42181 ggcgtttcaa agagtgtttg ttcgataccg aactgaaagc ggcacaggat tacgacatct
    42241 tcctgcggat ggtggtggag tatggcgaac cgtggaaagt ggaagaggcg acgcagatcc
    42301 tgcatatcaa tcacggtgaa atgcagatca cctcgtcacc gaaaaaattc tccggatact
    42361 tccactttta ccgcaagcac aaagacaaat tcgaccgtgc cagtaaaaaa tatcagctgt
    42421 ttaccctcta ccagatccgc aataaacgta tgacctggcg cactttgctg acgctgctgt
    42481 cggtgcgtaa cggcaaacgt ctggctgatg gtattcgggg gcgctaaata tgctggaaga
    42541 tctacgcgcc aacagctgga gtttacgccc gtgctgcatg gttcttgcct atcgcgtcgc
    42601 tcatttttgt tcggtgtggc gcaaaaagaa cgttctcaac aatctttggg cggccccgct
    42661 gctggtgctg tatcgcatta tcaccgaatg ctttttcggt tatgaaatcc aggctgccgc
    42721 gaccattggc cgccgcttta ctatccatca cggttacgcc gtagtgatca ataaaaacgt
    42781 ggtagcgggg gatgatttca ccattcgcca cggcgtcact atcggcaatc gtggtgccga
    42841 taacatggca tgtccacaca ttggcaacgg cgtcgaactc ggtgccaacg tcattattct
    42901 tggtgatatc acgcttggta acaacgtcac cgtgggcgcg ggcagcgtgg tgctcgactc
    42961 tgtcccggac aacgcgctgg tggtgggaga aaaagcgcga gtgaaggtaa ttaaatgaat
    43021 attctgcaat ttaatgtgcg actggcggaa ggcggggcgg caggtgtggc gttagatctc
    43081 caccagcgtg cgctgcaaca ggggctggcg tcacattttg tctacggcta cggcaaaggc
    43141 ggcaaagaga gcgtcagcca tcagaactat ccgcaggtca tcaaacatac gccgcggatg
    43201 accgcaatgg cgaatattgc cctgtttcgt ctgtttaatc gcgatctgtt tggcaatttc
    43261 aatgagttat atcgcaccat tactcgtaca ccgggtccgg tggtcctgca ttttcatgtg
    43321 ctgcacagct actggctaaa tcttaagagc gtggtgcgct tttgcgaaaa ggtgaaaaac
    43381 cacaaaccgg atgtcactct ggtctggacg ctgcacgacc actggagcgt taccgggcgc
    43441 tgcgccttta ccgacggttg tgaaggctgg aaaacgggct gccagaaatg cccgacctta
    43501 aataactatc cgccggtgaa gattgatcgg gcgcaccagc tggtggcggg caaacgccag
    43561 ttattccgtg agatgctggc gctgggctgt cagtttattt cccccagcca gcatgtggct
    43621 gacgctttca atagcctgta cggtccaggg cgttgccgga ttatcaataa cggtattgat
    43681 atggcaaccg aggcgattct ggcggatttg cctccggtgc gcgaaactca gggcaagccg
    43741 aaaatcgcgg tggtagcgca tgacctgcgt tacgacggca aaactaacca gcaactggta
    43801 cgcgagatga tggcgctggg cgacaaaatc gaactgcata cctttggtaa gttctcgccg
    43861 ttcaccgctg gcaacgtggt taatcacggc tttgaaactg acaagcgcaa gttgatgagc
    43921 gcgctcaatc agatggatgc gctggtgttc agttctcgcg tcgataacta cccgctgatt
    43981 ttgtgtgagg cgttatcaat tggcgtgccg gtgattgcca cccatagcga cgcggcgcgt
    44041 gaagtgttgc aaaaatccgg cggtaaaacc gtcagcgaag aagaggtgct gcaactggtc
    44101 cagttaagca aaccggaaat cgcgcaggcg ttatttggta ccacgctggc tgagttcagc
    44161 caacgcagcc gcgccgccta cagtggacaa cagatgctgg aggagtatgt caacttctat
    44221 cagaatctgt agctacctgc tgctgccgct gatctacctg ctggttaacg tcaaaatcac
    44281 ccagcttggc gaaagtttcc ccatcaccat cgtcactttt ttacctgtct tgttactgct
    44341 gtttttagaa cgcatcagcg ttaaaaaatt gatgattgcc ttagggattt gcgcgggact
    44401 cacggcgttt aactacctgt ttggtcagtc gctggatgcc agtaaatacg tcacttcaac
    44461 tatgctgttt gtctatatcg tgatcattat tggtatggtg tggagtattc gctttaaaac
    44521 gatttcgcca cacaaccatc gcaagatatt acgtttcttt tatctggtgg tcgggctggt
    44581 ggtggcgctg gcggcggtgg agatggcgca aattatcctc accggcggca gcagtattat
    44641 ggagtcgatt tcgaaatatc tgatttacag caacagctat gtgctgaatt tcatcaaatt
    44701 cggcggcaag cgcacgacag cactttattt cgaaccggca tttttcgctc tggcattaat
    44761 ctcaatttgg ctcagcatca aacagtttgg tatcaaaacg cctaaaacag atgctatgat
    44821 tctcgcaggg ataatattat ccggatcgtt ttcaggggtt atgaccttta tcctgtttta
    44881 tttgctggag tgggcatttc aatatctgaa taaagaggcg attaagaaaa agttaccgtt
    44941 agcattgatt tctctggctg tattcctggt tggtgtggta attgcatttc cttatatttc
    45001 cacccgtctg ggcgatttag gtacggaagg atcgtcatcg tattatcgta ttgtcggtcc
    45061 gctggtgatg gtcggttatt ctttgaccca tattgacggt gtagtcagat ttggctcact
    45121 ttatgaatat gtcgcatcat tcggaatatt taacggtgcg gatgtcggaa aaaccataga
    45181 caatggtttg tatctgctga ttatttattt ttcttggttc gcggtgtttt tatcgctgtg
    45241 gtacatgggg aaagtgataa aaatgatgat caacgctttt ggtgataacc gcaattttcg
    45301 cgtgcaatta tatcttttta ctccggtatc gctgtttttt accggttcga tatttagccc
    45361 ggaatatgca tttttaatcg tctgtccgtt tattttgcga aaagcgttaa atattacgag
    45421 gtaagaataa gaacatgttg cttagcataa tcactgtcgc gtttcgtaac ctcgaaggga
    45481 tagtcaaaac acatgcctcg ctggcgcatc tggcgcaggc ggaagatatc agcttcgaat
    45541 ggattgttgt cgatggcggt tccaatgacg gcactcgtga gtacctggaa aatctcaatg
    45601 gtatctataa cctacgcttt gtcagcgagc cagataacgg tatctacgac gccatgaaca
    45661 agggcattgc gatggcacaa ggcaagttcg cgttgtttct caactcgggc gatatttttc
    45721 atcaggatgc cgcatatttt gtccgtaagt taaaaatgca aaaagataac gtgatgatca
    45781 ccggcgatgc gctgctggat tttggcgacg ggcataaaat taaacgtagc gccaaaccgg
    45841 gctggtatat ttatcacagc ctgcccgcca gtcatcaggc gatatttttc ccggtatccg
    45901 gtttgaaaaa atggcgttat gacctggaat ataaagtttc ttctgattac gcgctggcag
    45961 ccaaaatgta taaagcaggt tatgcattta aaaaactcaa tggcctggtg tctgaatttt
    46021 ctatgggtgg ggtatctacc accaataata tggaattgtg tgctgacgcg aaaaaagtcc
    46081 aacggcaaat attacatgtg cctggctttt gggctgaatt atcctggcat ttacgccagc
    46141 gtactacctc aaagacgaaa gccttatata acaaaagctg aatataaggg aaaacgatgc
    46201 aggatttaag cggtttctcg gtgccgaaag ggttccgggg cggcaacgct attaaagtgc
    46261 aattgtggtg ggcagtacag gcaacaatat ttgcctggtc gccacaagta ttgtatcgct
    46321 ggcgggcatt tttattacgt ttattcggcg caaaaatagg aaaaaacgta gttattcgtc
    46381 cgtcagtaaa aattacctat ccgtggaaat taaccttagg tgattacgtg tgggtcggcg
    46441 atgacgtcaa tttatatacc ctcggtgaaa taaccattgg cgcacattcg gtgatatcgc
    46501 aaaaaagtta tttatgcacc gggagccacg accatgcaag tcaacatttc accattaacg
    46561 ccacgcctat tgtgattggc gagaaatgct ggctggcaac cgatgttttt gttgccccag
    46621 gcgtcacgat tggcgacggc accgtcgtgg gtgcgcgaag cagtgttttt aaatcgcttc
    46681 cggcaaatgt ggtttgccgg gggaatcccg cagtggtgat acgcaaacgc gttgaaactg
    46741 aataaattca aaaaaaatac agaggaataa gacatgtcaa aagtcgctct catcaccggt
    46801 gtaaccggac aagacggttc ttacctggca gagtttctgc tggaaaaagg ttacgaggtg
    46861 catggtatta agcgtcgcgc atcgtcattc aacaccgagc gcgtggatca catttatcag
    46921 gatccgcaca cctgcaaccc gaaattccat ctgcattatg gcgacctgag tgatacctcc
    46981 aacctgacgc gtattttgcg tgaagtgcag ccggatgaag tgtacaacct gggcgcaatg
    47041 agccacgttg cggtttcttt tgagtcaccg gaatataccg ctgacgtcga cgcgatgggt
    47101 acgctgcgcc tgctggaggc gatccgcttc ctcggtctgg aaaagaaaac ccgtttctat
    47161 caggcttcca cctctgaact gtatggtctg gtgcaggaaa ttccgcagaa agagaccacg
    47221 ccgttctatc cgcgctctcc gtatgcggtc gccaaactgt atgcctactg gatcaccgtc
    47281 aactaccgtg aatcctacgg catgtatgcc tgtaacggta ttctcttcaa ccatgaatcc
    47341 ccgcgccgcg gcgaaacctt cgttacccgc aaaatcaccc gcgcaatcgc caacatcgcc
    47401 caggggctgg agtcgtgcct gtacctcggc aatatggatt ccctgcgtga ctggggccat
    47461 gccaaagact acgtaaaaat gcagtggatg atgctgcaac aggaacagcc agaagatttc
    47521 gttatcgcga ccggcgttca gtactccgta cgtcagttcg tagaaatggc ggcagcacag
    47581 ctgggcatca aactgcgctt tgaaggcacg ggcgttgaag agaagggcat tgtggtttcc
    47641 gtcaccgggc atgacgcgcc gggtgttaaa ccgggtgatg tgattatcgc cgttgatccg
    47701 cgttacttcc gtccggctga agttgaaacg ctgctcggcg acccgaccaa agcgcacgaa
    47761 aaactgggct ggaaaccgga aatcaccctc agagagatgg tgtctgaaat ggtggctaat
    47821 gacctcgaag cggcgaaaaa acactctctg ctgaaatctc acggctacga cgtggcgatc
    47881 gcgctggagt cataagtatg agtagacaac gcatttttat tgctggccat cgcgggatgg
    47941 tcggttccgc catcaggcgg cagctcgaac agcgcggtga tgtggaactg gtattacgca
    48001 cccgcgacga gctgaacctg ctggacagcc gcgccgtgca tgatttcttt gccagcgaac
    48061 gtattgacca ggtctatctg gcggccgcga aagtgggcgg cattgttgcc aacaacacgt
    48121 atccggcgga tttcatctat cagaacatga tgattgagag caacatcatt cacgccgcgc
    48181 atcagaacga cgtgaacaaa ctgctgtttc tcggatcgtc ctgcatctac ccgaaactgg
    48241 caaaacagcc gatggcagaa agcgagttat tgcagggcac gctggagccg accaacgagc
    48301 cttacgccat tgccaaaatc gccgggatca aactgtgcga atcatacaac cgccagtacg
    48361 gacgcgatta ccgctcagtc atgccgacca acctgtatgg cccgcatgac aacttccacc
    48421 cgagtaattc gcatgtgatc cctgcattgc tgcgtcgctt ccacgaggcg acggcacaga
    48481 atgcaccgga cgtggtggtg tggggcagcg gtacgccgat gcgagaattt ctgcacgtcg
    48541 atgatatggc ggcggcgagc attcatgtca tggagctggc gcatgaagtc tggctggaga
    48601 acacccagcc gatgctgtcg cacattaacg tcggcacggg cgttgactgc actatccgcg
    48661 agctggcgca aaccatcgcc aaagtggtgg gttacaaagg tcgggtggtt tttgatgcca
    48721 gcaaaccgga tggtacgccg cgcaaactgc tggatgtgac gcgcctgcat cagcttggct
    48781 ggtatcacga aatctcactg gaagcggggc ttgccagcac ttaccagtgg ttccttgaga
    48841 atcaagaccg ctttcggggg taatgatgtt tttacgtcag gaagactttg ccacggtagt
    48901 gcgctccaca ccgcttgtct ctctcgactt tattgtcgag aacagtcgcg gcgagtttct
    48961 gcttggcaaa agaaccaacc gcccggcgca gggttactgg tttgtgccgg gagggcgcgt
    49021 gcagaaagac gaaacgctgg aagccgcatt tgagcggctg acgatggcgg aactggggct
    49081 gcgtctgccg ataacagcag gccagtttta cggtgtctgg cagcactttt atgacgataa
    49141 cttctccggc acggatttca ccactcacta tgtggtgctc ggttttcgct tcagagtagc
    49201 ggaagaagaa ctgttactgc cggatgagca gcatgacgat taccgctggc tgacgccgga
    49261 cgcgctgctc gccagtaacg atgttcacgc taacagccgc gcctattttc tcgctgagaa
    49321 gcgtgccgga gtacccggat tatgaaaata ctggtctacg gcattaacta ctcgccggag
    49381 ttaaccggca tcggcaaata caccggcgag atggtggaat ggctggcggc gcaaggtcat
    49441 gaggtgcggg ttatttccgc accgccttac tacccgcagt ggcaagtggg cgagaactat
    49501 tccgcctggc ggtacaaacg agaagagggg gccgctacgg tgtggcgctg cccgctgtac
    49561 gtgccaaaac agccgagcac cctgaaacgc ctgttgcatc tcggcagttt tgccgtcagc
    49621 agttttttcc cgttgatgga gcaacgtcgc tggaagccgg atcgcattat cggcgtagtg
    49681 ccaacgctgt tttgcacgcc gggaatgcgc ctgctggcga aactctctgg cgcgcgtacc
    49741 gtgctgcata ttcaggatta cgaagtagat gccatgctgg ggctgggcct tgccggaaaa
    49801 ggcaaaggcg gcaaagtggc acagctggca acggcgttcg aacgtagcgg actgcataac
    49861 gtcgataacg tttccacgat ttcgcgttcg atgatgaata aagccatcga aaaaggcgtg
    49921 gcggcggaaa acgtcatctt cttccccaac tggtcggaaa tcgcccgttt tcagcatgtt
    49981 gcagacgccg atgttgatgc ccttcgtaac cagcttggcc tgccggataa caaaaaaatc
    50041 attctttact ccggcaatat tggtgaaaag caggggctgg aaaacgttat tgaagcagcc
    50101 gatcgcctgc gcgatgaacc gctgattttt gccattgtcg ggcagggcgg cggcaaagcg
    50161 cggctggaaa aaatggcgca gcagcgtgga ctgcgcaaca tgcaattttt cccgctgcaa
    50221 tcgtatgacg ctttacccgc actgctgaag atgggcgatt gccatctggt ggtgcaaaaa
    50281 cgcggcgcgg cagatgccgt attgccgtcg aaactgacca atattctggc agtaggcggt
    50341 aacgcggtga ttactgctga agcccacaca gaactgggac agctttgcga aacctttccg
    50401 ggcattgcgg tttgcgtaga accggaatcg gttgaggcgc tggtggcggg gattcgtcag
    50461 gcgctcctgc tgcccaaaca caacacggtg gcacgtgaat atgccgaacg cacgctcgat
    50521 aaagagaacg tgttacgtca atttataaat gatattcggg gataattatg gcgcagtcga
    50581 aactctatcc agttgtgatg gcaggtggct ccggtagccg cttatggccg ctttcccgcg
    50641 tactttatcc caagcagttt ttatgcctga aaggcgatct caccatgctg caaaccacca
    50701 tctgccgcct gaacggcgtg gagtgcgaaa gccctgtggt gatttgcaat gagcagcacc
    50761 gctttattgt tgcggaacaa ctgcgtcaac tgaacaaact cactgagaac attattctcg
    50821 aaccggcagg gcgtaacaca gcaccggcca ttgcgctggc ggcgctggcg gcaaaacgtc
    50881 atagcccgga gaacgaccca ttaatgctgg tgctggcggc ggatcatgtg attgccgatg
    50941 aagacgcatt ccgcgccgcc gtgcgtaacg ctatgccgta tgccgaagcg ggcaagctgg
    51001 tgaccttcgg cattgtgccg gatctacctg aaaccggata tggctatatt cgtcgcggtg
    51061 aagtgtcggc gggtgagcag gatacggtgg cctttgaagt ggcgcagttt gtcgaaaaac
    51121 caaatctgga aaccgcgcag gcctatgtgg caagcggcga atattactgg aacagcggta
    51181 tgttcctgtt ccgtgccgga cgctatctcg aagaactgaa aaaatatcgc ccggatattc
    51241 tcgacgcctg tgaaaaagcg atgagcgccg tcgatccgga tctcgatttt attcgcgtgg
    51301 atgaagacgc gtttctggcc tgcccggaag agtcggtgga ttacgcggtc atggaacgta
    51361 cggcagatgc cgttgtggtg ccgatggatg cgggctggag cgatgtcggc tcctggtcct
    51421 cattatggga gatcagcgcc cacaccgccg agggcaacgt ttgccacggc gatgtgatta
    51481 atcacaaaac tgaaaacagc tatgtgtatg ccgaatctgg cctggtcacc accgtcgggg
    51541 tgaaagattt ggtggtagtg cagaccaaag atgcggtgct gattgccgac cgtaacgcgg
    51601 tgcaggatgt gaaaaaagtg gtcgagcaga tcaaagccga tggtcgccat gagcatcggg
    51661 tgcatcgcga agtctatcgc ccgtggggca aatatgactc tatcgacgcg ggcgaccgct
    51721 accaggtgaa acgcatcacc gtgaaaccgg gcgaaggctt gtcggtacag atgcaccatc
    51781 accgcgcgga acactgggtg gttgtcgcgg gaacggcaaa agtcaccatt gacggtgata
    51841 tcaaactgct tggtgaaaac gagtcgattt atattccgct gggggcgacg cactgcctgg
    51901 aaaacccggg gaaaattccg ctcgatttaa ttgaagtgcg ctccggctct tatctcgaag
    51961 aggatgatgt ggtgcgcttc gcggatcgct acggacgggt gtaaacgtcg catcaggcgc
    52021 tgattgtcgg atgcggcgtg aacgccttat ccgacctacg gttcggtttt gtaggcctga
    52081 taagacgcgg cagcgtcgca tcaggcaatg aatgcgaaac cgcggtgtaa ataacgacaa
    52141 aaacaaaatt ggccgtttcg gtcagggcca actattgcct gaaaaagggt aacgatatga
    52201 aaaaattaac ctgctttaaa gcctatgata ttcgcgggaa attaggcgaa gaactgaatg
    52261 aagatattgc ctggcgcatt ggtcgtgcct atggcgaatt tctcaaaccg aaaaccattg
    52321 tgttaggcgg tgatgcccgt ctcaccagcg aaaccttaaa actggcgctg gcaaaaggtt
    52381 tacaggatgc gggcgttgac gtgctggata ttggtatgtc cggcaccgaa gagatctatt
    52441 tcgccacgtt ccatctcggc gtggatggcg gcattgaagt tactgccagc cataacccga
    52501 tggattacaa cggcatgaaa ctggtgcgcg aaggggctcg cccgatcagc ggcgataccg
    52561 gactgcgcga tgtccagcgt ctggcagaag ccaacgactt tcctcccgtt gatgaaacca
    52621 aacgcggtcg ctatcagcaa atcaatctgc gtgacgctta cgttgatcac ctgttcggtt
    52681 atatcaacgt caaaaacctt acgccgctca agctggtgat caactccggg aacggcgcgg
    52741 cgggtccggt ggtggacgcc atcgaagccc gctttaaagc cctcggcgca ccggtggaat
    52801 tgatcaaagt gcacaacacg cccgacggca atttccccaa cggtattcct aacccgctgc
    52861 tgccggaatg tcgcgacgac acccgcaatg cggtcattaa acacggcgcg gatatgggca
    52921 ttgcttttga tggcgatttt gaccgctgtt tcctgtttga cgaaaaaggg cagtttatcg
    52981 agggctacta cattgtcggt ctgctggcag aagcgttcct cgaaaaaaat cccggcgcga
    53041 agatcatcca cgatccacgt ctctcctgga ataccgttga tgtggtgact gccgcaggcg
    53101 gcaccccggt aatgtcgaaa accggacacg cctttattaa agaacggatg cgcaaggaag
    53161 acgccatcta cggtggcgaa atgagcgccc accattactt ccgtgatttt gcttactgcg
    53221 acagcggcat gatcccgtgg ctgctggtcg ccgaactggt gtgcctgaaa ggaaagacgc
    53281 tgggcgaact ggtgcgcgac cggatggcag cgtttccgtc aagcggtgag atcaacagca
    53341 aactggcgca acccgttgag gcgattaacc gcgtggaaca gcattttagc cgtgaggcgc
    53401 tggcggtgga tcgcaccgat ggcatcagca tgacctttgc cgactggcgc tttaatctgc
    53461 gctcctccaa caccgaaccg gtggtgcggt tgaatgtgga atcacgcggt gatgtgccgc
    53521 tgatggaagc gcgaacgcga actctgctga cgttgctgaa cgagtaatgt cggatcttcc
    53581 cttaccccat tgcgggtaag gggctaataa caggaacaac gatgacaaat ctaaaaaagc
    53641 gcgagcgagc gaaaaccaat gcatcgttaa tctctatggt gcaacgcttt tcagatatca
    53701 ccatcatgtt tgccggacta tggctggttt gcgaagtgag cggactgtca ttcctctaca
    53761 tgcacctgtt ggtggcgctg attacgctgg tggtgttcca gatgctgggc ggcatcaccg
    53821 atttttatcg ctcatggcgc ggtgttcggg cagcgacaga atttgccctg ttgctacaaa
    53881 actggacctt aagcgtgatt ttcagcgccg gactggtggc gttcaacaat gatttcgaca
    53941 cgcaactgaa aatctggctg gcgtggtatg ggctgaccag tatcggactg gtggtttgcc
    54001 gttcatgtat tcgcattggg gcgggctggc tgcgtaatca tggctataac aagcgcatgg
    54061 tcgcggtggc gggggattta gccgccgggc aaatgctgat ggagagcttc cgtaaccagc
    54121 cgtggttagg gtttgaagtg gtgggcgttt accacgaccc gaaactgggc ggcgtttcta
    54181 acgactgggc gggtaacctg caacagctgg tcgaggatgc taaagcaggc aagattcata
    54241 acgtctatat cgcgatgcaa atgtgcgacg gcgcgcgagt gaaaaaactg gtccatcaac
    54301 tggcggacac cacctgttcg gtgctgctga tccccgatgt ctttaccttc aacattctcc
    54361 attcacgtct tgaagagatg aacggcgttc cggtggtgcc gctttacgac acgccgcttt
    54421 ccggggttaa ccgcctgctc aaacgtgcgg aagacattgt gctggcgacg cttatcctgc
    54481 tgctgatctc cccggtgctg tgctgcattg cgctggcggt gaaactcagt tcacctgggc
    54541 cggttatttt ccgccagact cgctacggca tggatggcaa gccgatcaaa gtgtggaagt
    54601 tccgttccat gaaagtgatg gagaacgaca aagtggtgac ccaggcgacg cagaacgatc
    54661 cgcgcgtcac caaagtgggg aactttctgc gccgcacctc gctggatgaa ttgccgcagt
    54721 ttatcaatgt gctgaccggg gggatgtcga ttgtcggtcc acgtccgcac gcggtggcgc
    54781 ataacgaaca gtatcgacag ctcattgaag gctacatgct gcgtcataag gtgaaaccgg
    54841 gcattaccgg ctgggcgcag attaacggct ggcgcggcga aaccgacacg ctggagaaaa
    54901 tggaaaaacg cgtcgagttc gaccttgagt acatccgcga atggagcgtc tggttcgata
    54961 tcaaaatcgt tttcctgacg gtattcaaag gattcgttaa caaagcggca tattgatatg
    55021 agcttacgtg aaaaaaccat cagcggcgcg aagtggtcgg cgattgccac ggtgatcatc
    55081 atcggcctcg ggctggtgca gatgaccgtg ctggcgcgga ttatcgacaa ccaccagttc
    55141 ggtttgctta ccgtgtcgct ggtgatcatc gcgctggcag atacgctttc tgacttcggt
    55201 attgctaact cgattattca gcgaaaagaa atcagccacc ttgaactcac cacgctgtac
    55261 tggctgaacg ttgggctggg gcttgtggtg tgtgtggcgg tgtttttgtt gagtgatctc
    55321 attggtgacg tgctgaacaa cccggacctg gcaccgttga ttaaaacatt gtcgctggcg
    55381 tttgtggtta tcccccacgg gcaacagttc cgcgcgttga tgcaaaagga gctggagttc
    55441 aacaaaatcg gcatgatcga aaccagcgcg gtactggcgg gcttcacttt tacggtggtt
    55501 agcgcccatt tctggccgct ggcgatgacc gcgatcctcg gttatctggt caatagtgcg
    55561 gtgagaacgc tgctgtttgg ctactttggc cgcaaaattt atcgccccgg tctgcatttc
    55621 tcgctggcgt ctgttgcgcc gaacttacgc tttggtgcct ggctgacggc ggacagcatc
    55681 atcaactatc tcaataccaa cctttcaacg ctggtgctgg cgcgtattct cggcgcgggc
    55741 gtggcagggg gatacaacct ggcgtacaac gtggccgttg tgccgccgat gaagctgaac
    55801 ccgatcatca cccgtgtgtt gtttccggca ttcgccaaaa ttcaggacga taccgaaaag
    55861 ctgcgcgtca acttctacaa gctgctgtcg gtggtgggga ttatcaactt tccggcgctg
    55921 ctcgggctga tggtggtgtc aaataacttt gtaccgctgg tatttggcga aaagtggaac
    55981 agcattattc cggtgctgca attgctgtgt gtggtgggtc tgctgcgctc cgtaggtaac
    56041 ccgattggtt cgctgctgat ggcgaaagcg cgggtcgata tcagctttaa attcaacgta
    56101 ttcaaaacat ttctgtttat tccggcgatt gttattggtg ggcagatggc gggcgcaatc
    56161 ggcgtcacgc tcggtttcct gctggtgcag attatcaaca ccattctgag ttacttcgtg
    56221 atgattaaac cggttcttgg ttccagttat cgccagtaca tcctgagttt gtggctgccg
    56281 ttttatctct cgctgccgac gctggtggtc agttatgtgc tgggcattgt gctgaaaggg
    56341 caactggcgc tggggatgct gctggcggtg caaatagccg cgggcgtgct ggcgtttgtg
    56401 gtgatgattg tgctgtcgcg ccatccgctg gtggtggaag tgaagcgcca gttttgtcgc
    56461 agcgaaaaaa tgaaaatgct tttacgggcg gggtgagttg ttgttttcgg ttccgacaac
    56521 ggttgccggg tgcagcgtaa aacaccttat ccatcctacg gttctgtgct cgggtcttgt
    56581 aggcctgata agacgcgcca gcgtcgcatc aggcaaaaaa cggcaacgaa cacaacaaac
    56641 acagcctcac ctggcatcaa atttatgagg aaaaaatgaa attacttatt ctgggcaacc
    56701 acacttgcgg caatcgtggc gacagcgcca tcttgcgcgg cttacttgat gccatcaaca
    56761 tcctcaatcc acataccgaa gtggacgtga tgagccgcta tccggtcagt tcttcctggc
    56821 tgctcaaccg cccggtaatg ggtgatccgc tgttccttca aatgaaacaa cacaacagcg
    56881 cggcgggcgt tgtcgggcgc gttaaaaaag tcctccgtcg ccgctatcag caccaggtat
    56941 tgctctcacg cgtcaccgac actggcaagc tgcgcaatat cgccatcgcc cagggattca
    57001 ccgacttcgt gcgcctactg tcaggttacg acgccattat tcaggtcggc ggatcgtttt
    57061 ttgtcgatct ctacggcgtg ccgcagtttg aacatgcact ttgcacattt atggcgaaaa
    57121 agccgctgtt tatgattggt cacagcgtcg gtcccttcca ggatgagcaa tttaaccaac
    57181 tggcgaacta cgtttttggt cactgcgacg cgctgatctt gcgcgaatcg gtcagcctcg
    57241 atctaatgaa acgcagcaat atcaccactg caaaagttga acatggcgtc gataccgcgt
    57301 ggctggtcga tcaccacaca gaagacttca ccgccagcta tgccgtccaa cactggctgg
    57361 acgttgccgc acaacagaaa acggtggcaa ttaccctgcg cgaactggca ccgttcgaca
    57421 aacgcctcgg caccactcag caagtgtatg aaaaagcctt tgccggggtg gtcaatcgca
    57481 ttctcgacga aggctatcag gtcattgcgc tctccacctg tacgggtatc gacagctata
    57541 acaaagacga ccgcatggtg gcgctcaacc tgcgccagca catcagcgat cctgcccgtt
    57601 atcacgtagt gatggatgaa cttaacgatc tggaaatggg caaaattctc ggtgcctgtg
    57661 aactcaccgt cggtacgcgc ctgcactccg ccattatctc aatgaacttt gctactccgg
    57721 cgattgccat caactatgaa cataaatccg ccggggttat gcagcagctg ggactaccgg
    57781 agatggcaat tgatatccgt catttattag acggcagcct gcaagcgatg gttgcggata
    57841 ccttaggcca gcttccggcg ctgaacgcac gacttagcga agccgtcagt cgtgagcgtc
    57901 agacgggaat gcagatggtg cagtccgtac tggaacgcat cggggaggtg aaatgaaggt
    57961 cggcttcttc ttattgaaat ttccgctgtc gtcagaaacc ttcgttctta accaaatcac
    58021 tgcgtttatt gatatgggct ttgaagtgga gattgtcgcg ctgcaaaaag gcgacacaca
    58081 aaacacccac gtggcatgga ctaaatacaa ccttgccgcc agaacccgct ggttacagga
    58141 cgaacctacg ggcaaagtgg cgaaactgcg ccaccgagcc agtcagacct tgcgcggcat
    58201 tcatcgtaaa aatacctggc aggcgctcaa cctcaaacgc tatggtgccg agtcgtggaa
    58261 cctgattttg tctgccattt gcggccaggt cgcaacaccg tttcgcgccg atgtgttcat
    58321 cgctcatttt ggccctgcgg gggtaaccgc agcaaaacta cgcgaactgg gtgtcattca
    58381 cggcaaaatt gccactattt tccacggcat tgatatttcc agtcgggaag tgctcaacca
    58441 ttacactccc gaatatcaac aactgtttcg acgtggcgac ctgatgctac cgataagcga
    58501 tctgtgggcc ggaaggctgc aaaaaatggg ctgcccgagg gaaaaaatcg ccgtatcgcg
    58561 catgggcgta gatatgacgc gctttagccc gcgtcccgtg aaagcgcccg caacaccgct
    58621 ggagattatt tccgtcgcac gcttaaccga gaaaaaaggt ctgcatgtgg cgattgaagc
    58681 ctgccggcag ttgaaagagc agggcgtggc atttcgctat cgcatcctcg gcattggccc
    58741 gtgggaacga cgcctgcgca cgctcatcga acaatatcaa ctggaagatg tggtggagat
    58801 gccgggcttt aaaccgagcc atgaagtgaa agcgatgctc gacgacgcgg atgtcttcct
    58861 gttgccatcg gttacaggtg cggatggcga tatggaaggc attccggtgg cgctgatgga
    58921 ggcgatggcg gtcggcattc cggtggtttc tactctgcat agcggaatac cggaactggt
    58981 ggaggctgac aaatccggct ggctggtgcc tgagaacgat gctcgcgcac tggcgcaacg
    59041 actggcggcg tttagccaac tggacaccga cgaattggct ccggtcgtca aacgcgcgcg
    59101 cgaaaaagtt gaacacgatt ttaaccagca ggtgatcaat cgagaactcg ccagcttgct
    59161 gcaggcttta tagaggagaa cgtatgccat ttaaaaaact ctcccgacgc accttcctga
    59221 cggcaagctc ggcgcttgcc ttccttcata cccctttcgc tcgcgcactt cccgcccgac
    59281 aaagcgttaa tatcaacgac tacaaccctc acgactggat cgcctcattt aaacaagcct
    59341 tcagcgaagg gcaaacggtt gtcgtgcctg ctggattcgt ttgtgacaat atcaacaccg
    59401 gcatcttcat tcctcctggc aaaacgttac acatccttgg aagcctgcgc ggcaacggca
    59461 gagggcgatt tgtcttacag gacggcagcc aggtgacagg ggaggagggc ggcagtatgc
    59521 ataacatcac cctggatgtg cgtggttctg actgcaccat caaagggctg gcgatgagcg
    59581 gctttggtcc ggtaacgcag atttatatcg gcggcaaaaa caaacgggtc atgcgcaacc
    59641 tgaccatcga taacctgacc attagccacg ctaattacgc catcttacgc cagggatttc
    59701 ataaccagat tatcggtgcc aacatcacca attgtaagtt cagcgactta cagggcgatg
    59761 ccattgaatg gaacgtggcg attaacgacc gcgatatttt gatctccgac catgtcatcg
    59821 agcgcatcaa ctgtactaac ggcaaaatca actggggcat tggcataggc cttgcgggaa
    59881 gcacttacga taataattac ccggaagatc aggcagtgaa aaactttgtc gtggcgaata
    59941 tcacgggatc ggattgtcgg cagttgatac atgttgaaaa tggtaaacat tttgttattc
    60001 gtaatatcaa agcccgcaat atcacgcagg atttcagtaa gaaagcaggt attgataacg
    60061 ccacagtcgc tatttacggt tgtgacaatt tcgtgattga taatattgaa atgattaata
    60121 gcgccgggat gttaatcggc tatggggtaa ttaaaggcaa ttatctctcg attccgcaaa
    60181 atttccgagt gaataatatt caactggata acacccatct tgcttataaa ttgcgcggcc
    60241 tccaaatctc tgccgggaat gccgtctcct ttgtggcact aactaacatt gagatgaagc
    60301 gtgcgtcgct ggagttacac aacaaaccgc aacatctttt tctgcgtaat atcaaagtga
    60361 tgcaggaatc ctctgttgga cccgcattga ttatgaactt cgacatgcgc aaagacgttc
    60421 gaggcgtctt tatggcgaaa gaagaaacac tgctgtctct tgcaaatgtt catgcggtga
    60481 atgagaaagg acaaagctcc gtcgatatcg acaggattaa tcaccatatt gttaatgtgg
    60541 aaaagattaa ctttagattg ccggaacgga gagagtagat ttgcgaccat tcataaactt
    60601 actggatata atgttctaaa agattataat cctcacacta actgttaaag ctggggctgg
    60661 gcagcattga ctactcaggg aaggtactca tctttatctt gtgtttctta cgtaacaacc
    60721 agactaaagt gatttatgaa cgataacgtt ttgctcatag gagcttccgg attcgtagga
    60781 acccgactac ttgaaacggc aattgctgac tttaatatca agaacctgga caaacagcag
    60841 agccactttt atccagaaat cacacagatt ggtgatgttc gtgatcaaca ggcactcgac
    60901 caggcgttag ccggttttga cactgttgta ctactggcag cggaacaccg cgatgacgtc
    60961 agccctactt ctctctatta tgatgtcaac gttcagggta cccgcaatgt gctggcggcc
    61021 atggaaaaaa atggcgttaa aaatatcatc tttaccagtt ccgttgctgt ttatggtttg
    61081 aacaaacaca accctgacga aaaccatcca cacgaccctt tcaaccacta cggcaaaagc
    61141 aagtggcagg cggaggaagt gctgcgtgaa tggtataaca aagcaccaac agaacgttca
    61201 ttaactatca tccgtcctac cgttatcttc ggtgaacgca accgcggtaa cgtctataac
    61261 ttgctgaaac agatcgctgg cggcaagttt atgatggtgg gcgcagggac taactataag
    61321 tccatggctt atgttggaaa cattgttgag tttatcaagt acaaactgaa gaatgttgcc
    61381 gcaggttacg aggtttataa ctacgttgat aagccagacc tgaacatgaa ccagttggtt
    61441 gctgaagttg aacaaagcct gaacaaaaag atcccttcta tgcacttgcc ttacccacta
    61501 ggaatgctgg gtggatattg ctttgatatc ctgagcaaaa ttacgggcaa aaaatacgct
    61561 gtcagctctg tgcgcgtgaa aaaattctgc gcaacaacac agtttgacgc aacgaaagtg
    61621 cattcttcag gttttgtggc accgtatacg ctgtcgcaag gtctggatcg aactctgcag
    61681 tatgaattcg tccatgccaa aaaagatgac ataacgtttg tttctgagta atgcagacga
    61741 gtcagagaaa aaaggaccag gttggactac gattattcgg aaagaggtac tgattaaatt
    61801 atcagtatgt ggatttccga gtaatccgag taacccagat gctgatggtg tgtagtgtta
    61861 tcgtaaggtt taatcaaagt tatgcggaac taagctttgg tatatagcca taacgtttgg
    61921 ctaacctatg gcaataattt acgcactatc agccacttag aggtaggaaa agaatgacga
    61981 atttaaaagc agttataccg gtagcaggtc ttgggatgca tatgttgcct gccactaagg
    62041 cgattcccaa agagatgcta ccgatcgtcg acaagccaat gattcagtac attgttgacg
    62101 agattgtggc tgcagggatc aaagaaatcc tcctggtaac tcacgcgtcc aagaacgcag
    62161 tcgaaaacca cttcgacacc tcttatgaat tagaatctct ccttgaacag cgcgtgaagc
    62221 gtcaactact ggcggaagta caatctatct gtccgccggg cgtgaccatt atgaacgtgc
    62281 gtcagggtga acctttaggt ttaggccact ccattttatg tgcgcgacct gccattggtg
    62341 acaatccatt tgtcgtggtg ctgccagacg ttgtgatcga cgacgccagc gccgacccgc
    62401 tacgctacaa ccttgctgcc atgattgcgc gcttcaatga aacgggacgc agccaggtgc
    62461 tggcaaaacg tatgccgggc gacctctctg aatactccgt catccagacc aaagaaccgc
    62521 tggatcgtga aggcaaagtc agccgcattg ttgaatttat cgaaaagccg gatcagccgc
    62581 agacgctgga ctcagacatc atggccgttg gtcgatatgt gctttctgcc gatatttggc
    62641 cggaactgga acgtactcag cctggtgcat ggggacgtat tcagctgact gatgccattg
    62701 ctgagctggc aaaaaaacaa tccgttgatg caatgctgat gactggcgac agttacgact
    62761 gcggtaaaaa aatgggctat atgcaggcgt ttgtgaagta tggactacgc aacttgaaag
    62821 aaggggcgaa gttccgcaaa ggtatcgaga agctgttaag cgaataatga aaatctgacc
    62881 gaatgtaacg gttgataagg aaataatatc ggcagtgaaa aactatagat caataaatat
    62941 cactgctgtt gagcttttat aattaactat gctaccaatg aataaatagt gtgcagatat
    63001 gtattttttg tttggtagtc ttgtttctcc ctctgaataa taagataata aacgcttgga
    63061 gatgattttt ttaacatgga ttatcatttc gtacactggt agcgattaag ccaggggcgg
    63121 tagcgtgcat aaattttaat gcttatcaaa actattagca ttaaaaatat ataagaaatt
    63181 ctcaaatgaa caaagaaacc gt
//
LOCUS       DAZFBR010000014        58669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFBR010000014 DAZFBR010000000
VERSION     DAZFBR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.014"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            102..1118
                     /locus_tag="ACHJZ2_001065"
     CDS             102..1118
                     /locus_tag="ACHJZ2_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO4567223.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            1203..1625
                     /locus_tag="ACHJZ2_001066"
     CDS             1203..1625
                     /locus_tag="ACHJZ2_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4567224.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            1622..1825
                     /locus_tag="ACHJZ2_001067"
     CDS             1622..1825
                     /locus_tag="ACHJZ2_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567225.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(1829..2236)
                     /gene="cueR"
                     /locus_tag="ACHJZ2_001068"
     CDS             complement(1829..2236)
                     /gene="cueR"
                     /locus_tag="ACHJZ2_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO4567226.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(2233..3408)
                     /locus_tag="ACHJZ2_001069"
     CDS             complement(2233..3408)
                     /locus_tag="ACHJZ2_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO4567227.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            complement(3405..5567)
                     /locus_tag="ACHJZ2_001070"
     CDS             complement(3405..5567)
                     /locus_tag="ACHJZ2_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO4567228.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            complement(5571..20111)
                     /locus_tag="ACHJZ2_001071"
     CDS             complement(5571..20111)
                     /locus_tag="ACHJZ2_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO4567229.1"
                     /translation="MDINTIATDDIINASEKGADLQISGTSNQPAGTTITLALNGQNY
                     TATTDAAGNWSTTVPASAVGALGEASYTVTANVTDSAGNSNSASHNVQVNTALPGVTI
                     NPVATDDIINAAESGNAQTISGQVTGAAAGDTVTVTLGGKTYTATVQGNLSWSVDVPA
                     ADIQAIGNGNLTVNASVTNGVGNTGSGSRDITIDANLPGLRVDTVAGDDVVNSIEHAQ
                     ALVITGSSSGLAAGAALTVVINTVTYAATVLADGTWSVGVPAADVSNWPAGTVNITVS
                     GTNTAGTTSTITHPVTVDLAAVAISINTVSGDDVINAAEKGADLTLSGSTSGVEVGQT
                     VTVTFGGKTYTATVAGDGSWTTTVPAADLSVLRDGDATVQASVSTINGNTASATHAYS
                     VDATAPTLAINTIATDDILNAAEAGNPLTISGSSTAEAGQTVTVTLNGVTYSGSVQAD
                     GSWSVSLPTADLSNLTASQYTVSASVSDKAGNPASANHGLAVDLTVPVLTINTVSGDD
                     IINAAEHGQALVISGSSTGGEAGDVITVTLNSKTYTTMLDASGNWSVGVPAADVTALG
                     SGPQTITAAITDAAGNSDDASRTVTVNLAAPTIGINTIATDDVIKATEKGADLQITGT
                     SNQPAGTTITVTLNGQNYTATTDSNGNWSATVPASAVSALGEANYTVTANVTDTAGNS
                     NSASHNVLVNSALPAVTINAVATDDIINAAESGNAQTISGQVTGAAQGDTVTVTLGGN
                     TYTATVQSNLSWSVDVPAADIQALGNGDLTVNASVTNGVGNTGSGSRDITIDANLPSL
                     RVDTVAGDDVINSIEHNQALVITGSSSGLTAGTALTVEINNVTYGATVLADGTWSLGV
                     PAVDVSNWPAGTVNITVSGTNSAGTTSTITHPVTVDLAGVAITINTLSGDDVINAVEK
                     GETLVVSGSTSGVEAGQTVTVTFGGKNYTTTVEANGSWTVNVPPADLAALPDGAGNVQ
                     ASVSNINGNSAQADRAYSVDATAPLVTINTIASDDILNVSEAGAGITISGTTTAQAGQ
                     TLTVTLNNNTYQTTVLADGTWSVNVPAADLSGLTASSYTVTATVSDKAGNPASADHAL
                     VVDITAPDLTINTVAGDDIINAIEHGQALVVSGTSTGAAAGDVVTVTLNGKNYTTTLD
                     ASGNWSVGIPAADVTALATGSQTITASLSDRAGNSDSTTHDVTVDLSGPTLTINTVSG
                     DDIINAAEIVVAQTISGQVTGTAVAGNTVIVTIGGNQYNATVQSDLSWSVSVPANVLQ
                     ALGNGELTISASLTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSIEHTQALVI
                     TGSSSGLAAGAALTVVINSVTYGATVLADGSWSVGVPVADVTNWPAGTVNIAVSGTNT
                     AGTTTSISHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSGVEAGQTITVI
                     FGGKSYTTTVAADNTWGLTIPAVDVATLPDGAANVQASVSNVAGNSTQATHAYSVDAT
                     APSVTINTIATDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGNVQADGSWS
                     VSVPTGDLASLTASSYTVNASVSDKARNSASATHNLTVDLAAPVVTINTVAGDDIINA
                     TEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVISALAQGDV
                     TITATVTDSAGNSGTASHTVTVALGAPVLAINTIAVDDIINAAEKGADLAITGTSNQP
                     AGTQITVTLNGQNYTTTADASGNWSVTVPASRVSALGEATYTVTAAATDADGNSGSAS
                     HNVQVNTALPGVTINVVATDDIINAAEAGVEQTISGQVTGAAAGDTVTVTLGGATYTA
                     TVQANLSWSVDVPASALQELGNGELTISASVTNSVGNTGNGTREITIDANLPGLRVDT
                     VAGDDVVNIIEHGQALVITGSSSGLAAGSNVTLTINGQTYVAAVLADGTWSVGVPAVD
                     VSAWPAGSVTIAASGSTSAGNPVSVTHPVTVDLSAVAVSINAITADDVINAAEKGAAL
                     TLSGSTSGVEAGQTVTVTFGGKTYSATVAANGSWSTSVPAADMAALRDGDASAQASVS
                     NVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTAEAGQTVTV
                     TLNGTNYTGTVQTDGSWSVSVPSADLSTLTASNYTVNAAVSDKAGNPASVNHNLTVDT
                     SVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFTTVLDASGN
                     WSVGVPASVVSALANGTVTINASVTDAGGNSGSATHQVTVNTGLPTITFNAISGDNIL
                     NADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVSDLAALGQA
                     NYTVSASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAGADQTISGVVT
                     RAAAGDTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITASVTNANGNTG
                     SGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVLTVTINSVA
                     YSATVQADGSWSVGIPAANVSAWPAGPLTVEVDGQSSANNPVSVSHPFTVDLTAVAIS
                     INTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAANGSWSVNVP
                     AADLATLPEGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDDILNAAEAG
                     SPLTISGTSTAETGQTVTVTLNGATYTGTVQADGSWSVSVPTSALGALNASNYTVSAT
                     VNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTSSGGEAGDV
                     VSVVLNGKTYTTTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEYS
                     VDSAAPTVTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQTD
                     GSWSLTLPASDLTALANNGYTLTATVSDLAGNLGSASKGVTVDTTAPVISFNTVAGDD
                     VINNVEHIQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGLA
                     DGTVTISATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNLINAAEAGSALTLSGT
                     GTNFATGTVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSAG
                     NSATASRSVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFGG
                     KTYTATVAANGTWALNVPAVDLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAPT
                     VTIATVAGDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQTWSATVGSGGSWSVFI
                     PAQQFAGLSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAEH
                     GSSLVISGTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVIN
                     ASVSNAIGNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSLT
                     AALASNETAQISIDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGAT
                     DSQNVVIDTTAPDPAVKTIAISAITTDMGLITNDFVTSDTTLAVSGTLGATLSAGEFA
                     QISLDGGVTWTTLTVVGTSWSYADGHTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDT
                     TSPEAAKSITITGISDDTGTSSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATW
                     VNVTVAADSLNWSYVDGRTLTNGTTTWQVRVVDLAGNVGATSSQSALIDTVNPAQVLT
                     IASISTDTGSSATDFITSDTMLTLTGSLGAGLASGEVAQISLDSGATWTTLTTNGTQW
                     TYTDSRTLTDGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQ
                     GTLSSSQATDDTTPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLV
                     SGAYTYSARVVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAAL
                     ASGQYVEVVINGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSS
                     AGNGNNANISNGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQS
                     TDPLTWSKTALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGT
                     FSSAIQVTVGTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTT
                     SNSGGSATVGGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGT
                     FVWGQNTTNTFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTN
                     GVLGSPVAVGATATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVSNASNWTQS
                     ALGGSQSGTTSGVAAMDYDWDGAVDVLVSKQSGSVFLSRNTNTVSYGTSLHLRITDPN
                     GINVYYGNTVKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKS
                     TGTTASNIDQTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGT
                     DTYDGSGGWVYSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNI
                     EGISGSNFNDILTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDV
                     VNGFTVGTWEGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVT
                     QSGSDTIVQIDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            complement(20148..24533)
                     /locus_tag="ACHJZ2_001072"
     CDS             complement(20148..24533)
                     /locus_tag="ACHJZ2_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO4567230.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            complement(24636..25991)
                     /locus_tag="ACHJZ2_001073"
     CDS             complement(24636..25991)
                     /locus_tag="ACHJZ2_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFO4567231.1"
                     /translation="MARFQFKNRKNNGLIFFISFMVMGEAAIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPGVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
     gene            complement(26329..27621)
                     /gene="ybaT"
                     /locus_tag="ACHJZ2_001074"
     CDS             complement(26329..27621)
                     /gene="ybaT"
                     /locus_tag="ACHJZ2_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO4567232.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(27624..28556)
                     /gene="glsA"
                     /locus_tag="ACHJZ2_001075"
     CDS             complement(27624..28556)
                     /gene="glsA"
                     /locus_tag="ACHJZ2_001075"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO4567233.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            28818..31322
                     /gene="copA"
                     /locus_tag="ACHJZ2_001076"
     CDS             28818..31322
                     /gene="copA"
                     /locus_tag="ACHJZ2_001076"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO4567234.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(31537..31878)
                     /gene="ybaQ"
                     /locus_tag="ACHJZ2_001077"
     CDS             complement(31537..31878)
                     /gene="ybaQ"
                     /locus_tag="ACHJZ2_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO4567235.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            32016..32810
                     /gene="ybaP"
                     /locus_tag="ACHJZ2_001078"
     CDS             32016..32810
                     /gene="ybaP"
                     /locus_tag="ACHJZ2_001078"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO4567236.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            33014..33493
                     /gene="ybaK"
                     /locus_tag="ACHJZ2_001079"
     CDS             33014..33493
                     /gene="ybaK"
                     /locus_tag="ACHJZ2_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO4567237.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(33530..35182)
                     /gene="ushA"
                     /locus_tag="ACHJZ2_001080"
     CDS             complement(33530..35182)
                     /gene="ushA"
                     /locus_tag="ACHJZ2_001080"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO4567238.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            35400..36620
                     /gene="fsr"
                     /locus_tag="ACHJZ2_001081"
     CDS             35400..36620
                     /gene="fsr"
                     /locus_tag="ACHJZ2_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO4567239.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            36857..38533
                     /gene="ybaL"
                     /locus_tag="ACHJZ2_001082"
     CDS             36857..38533
                     /gene="ybaL"
                     /locus_tag="ACHJZ2_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO4567240.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(38666..39970)
                     /gene="gsk"
                     /locus_tag="ACHJZ2_001083"
     CDS             complement(38666..39970)
                     /gene="gsk"
                     /locus_tag="ACHJZ2_001083"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO4567241.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            40122..41081
                     /gene="aes"
                     /locus_tag="ACHJZ2_001084"
     CDS             40122..41081
                     /gene="aes"
                     /locus_tag="ACHJZ2_001084"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO4567242.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(41078..42040)
                     /gene="hemH"
                     /locus_tag="ACHJZ2_001085"
     CDS             complement(41078..42040)
                     /gene="hemH"
                     /locus_tag="ACHJZ2_001085"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO4567243.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(42172..42816)
                     /gene="adk"
                     /locus_tag="ACHJZ2_001086"
     CDS             complement(42172..42816)
                     /gene="adk"
                     /locus_tag="ACHJZ2_001086"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO4567244.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(42997..44871)
                     /gene="htpG"
                     /locus_tag="ACHJZ2_001087"
     CDS             complement(42997..44871)
                     /gene="htpG"
                     /locus_tag="ACHJZ2_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO4567245.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(44981..45586)
                     /gene="recR"
                     /locus_tag="ACHJZ2_001088"
     CDS             complement(44981..45586)
                     /gene="recR"
                     /locus_tag="ACHJZ2_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO4567246.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(45586..45915)
                     /gene="ybaB"
                     /locus_tag="ACHJZ2_001089"
     CDS             complement(45586..45915)
                     /gene="ybaB"
                     /locus_tag="ACHJZ2_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO4567247.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(45968..47899)
                     /gene="dnaX"
                     /locus_tag="ACHJZ2_001090"
     CDS             complement(45968..47899)
                     /gene="dnaX"
                     /locus_tag="ACHJZ2_001090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO4567248.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    complement(46571..46635)
                     /gene="dnaX"
                     /locus_tag="ACHJZ2_001090"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(48028..48579)
                     /gene="apt"
                     /locus_tag="ACHJZ2_001091"
     CDS             complement(48028..48579)
                     /gene="apt"
                     /locus_tag="ACHJZ2_001091"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO4567249.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(48732..49109)
                     /gene="ybaN"
                     /locus_tag="ACHJZ2_001092"
     CDS             complement(48732..49109)
                     /gene="ybaN"
                     /locus_tag="ACHJZ2_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO4567250.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            49179..49706
                     /gene="priC"
                     /locus_tag="ACHJZ2_001093"
     CDS             49179..49706
                     /gene="priC"
                     /locus_tag="ACHJZ2_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO4567251.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            49720..49884
                     /gene="rsmS"
                     /locus_tag="ACHJZ2_001094"
     CDS             49720..49884
                     /gene="rsmS"
                     /locus_tag="ACHJZ2_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO4567252.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(49923..53285)
                     /gene="mscK"
                     /locus_tag="ACHJZ2_001095"
     CDS             complement(49923..53285)
                     /gene="mscK"
                     /locus_tag="ACHJZ2_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO4567253.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(53413..54060)
                     /gene="acrR"
                     /locus_tag="ACHJZ2_001096"
     CDS             complement(53413..54060)
                     /gene="acrR"
                     /locus_tag="ACHJZ2_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO4567254.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            54202..55395
                     /gene="acrA"
                     /locus_tag="ACHJZ2_001097"
     CDS             54202..55395
                     /gene="acrA"
                     /locus_tag="ACHJZ2_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO4567255.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            55418..58567
                     /gene="acrB"
                     /locus_tag="ACHJZ2_001098"
     CDS             55418..58567
                     /gene="acrB"
                     /locus_tag="ACHJZ2_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO4567256.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
BASE COUNT        12426 a        16210 c        16126 g        13907 t
ORIGIN      
        1 gtagatttcg attaaattgc aacgcagttt atttcttaaa acaatattat ttgtttctta
       61 tagaaacatt aatacgactt attttgaaca agagaaaatg aatgaaaact gtaaacgtag
      121 ctttactggc actcataatt tcagcaacat ccagccctgt tgttttagct ggtgatacca
      181 ttgaagcggc ggcaacagag ctttcagcca ttaactctgg catgtcgcaa tcggagattg
      241 agcagaagat tacccgcttt ttagaacgca cagacaacag ccccgctgcg tatacctatt
      301 tgactgaaca tcactacatc ccttctgaaa cacctgatac cactcagact cccactgtcc
      361 agacagatcc tgacgcagga caaaaaaccg ttgccgctac aggtgatgta cagacaactg
      421 cccgttatca gagcatgatc aacgcccgac agtctgcggt aactgacgcc cagcaaacgc
      481 aaattacaga gcaacaggcg cagatcgtag ccacacaaaa aacgctcgcc gcgactggag
      541 atacgcaaaa taccgcgcat tatcaggaaa tgattaatgc cagactggcg gctcaaaatg
      601 aggctaatca gcgcaccgcc actgaacaag ggcagaaaat gaatgcgctg acaaccgatg
      661 tggcagtaca acagcaaaat gaaaggactc aatacgataa acaaatgcaa agtctggcgc
      721 aggagtctgc ccaggcacat gaacaaattg acagcctgtc acaagacgta acccaaacgc
      781 accaacagtt aaccaacacc caaaaacggg ttgcagataa cagccagcaa attaacacgc
      841 tcaataacca tttcagttcg ctaaaaaacg aagttgatga caatcgtaaa gaagccaatg
      901 cgggaactgc atctgccatc gctatcgcct cacaaccaca ggttaaaacc ggtgacgtga
      961 tgatggtgtc agcgggagcg ggaaccttca acggtgaatc tgcggtgtct gtcggaacat
     1021 catttaatgc cggaacgcat acggtactta aagccggtat ttctgcggat acacaatctg
     1081 atttcggcgc aggtgtcggc gtgggatatt cgttctaata tttcaatcct caatataaat
     1141 aagagcaagg aagcttgccg ggttcacctc ttcattaatt tgtacattat ttaaggttaa
     1201 caatgatgaa tagctccatt aaatcgtttt ccctgctggc ggttatatta ctggctggct
     1261 gtagttcacc cacttcccgc atcgcagatt gccaggcgca gggcgtcagt catgacacct
     1321 gttacctcgc agaacagcag cgtcaggcgg ctattttaag tgcatccgag gcacaggcat
     1381 ttaaaaatgc agaagccgca caacacgccc aggcggcaaa gaaagccatt tataaaggat
     1441 ttggcatgac ctttagaatg agcagtaaaa actttgctta tctcaatgat tcattatgtg
     1501 caattgatga agacaataaa gatgccactg tttatcagtc aggtctatat aacgtcattg
     1561 tttatcatca cacaggaaaa gtcgccttaa tgaaagaagg ccagtttgtg ggttatttaa
     1621 aatgaaggag caaaggaaaa tacccctgac gcatattatg attatcggtg cgtttatttt
     1681 tgccttcttg caagtagtat tattagcctc cctggttcac gctgtgaatg ttaacaacga
     1741 aatccaggaa ggcttatttc agtcggggcg cattatggta gaaagtttgc agcatattct
     1801 ttcggtgcaa acggggattc actgattttc accccgcccg atgatgacag cagccggaga
     1861 gattttcgat aatcgggcag tcggcgctgt catcaccggg gcaggcattc gccagcgcca
     1921 gtagctggtt gcgcatagat tgcagttctt cgatatgccg ttcaatctcc gccaccttct
     1981 ccagcgtgcg acgtttgacg tcggcactgt gacgctgcgg gtcgttaaac agattcacca
     2041 gctcgccgct ctcttccagg ttaaagccca cctggcgcgc ctggcgcagt aaggtcaatt
     2101 cgttgagatg ctgctgcgtg taggttcgat aaccattttc gctgcgcatc ggcggcgtca
     2161 ccagcccctt ctcttcatag aagcgaatgg ctttgctggt caggccggta atttttgcta
     2221 catcgctaat gttcatcgtt cgcgcaacgc ctcctgtgct ttgttaaagg gtttcaacag
     2281 ataatccagc acgctttttt tgccggtgcg gatatcgacc gtcgccacca taccggggaa
     2341 tatcggaaac tgctgtttgc ttttgttttc cagatggttg ctgaaagtgc gaatgtacac
     2401 ccgatagtaa taaacatcgc gccgcacttc atcctgcacg gtgtctggtg atatcaccgc
     2461 cacttctccc ggcaggccgc cgtagatgga gtaatcgtag gcagttattt tcaccagcga
     2521 tttttgcccc gggtggataa acgccacatc gcgcggggag atttttgcct caatcagcag
     2581 ttgctcatcc agcggcacga tggtcatcag tttaccaccg ggggcgatga cgccgccaac
     2641 cgtggtgaca tcaatatcct gaacaatgcc tcgcaccggc gcggtaaagt tgagcctggt
     2701 gagagagtct tcccgtccac ggatcaccga acgctgggtt tccacttccg cattggcttt
     2761 tgctaactct tcgcgcgcct gaacgtagta ttgcgtgcgc acgtcgctga gtttgttttc
     2821 cagttcattg gcctggcgtt gcaggcgcag tacttcgacg ctgctcgctg ccccctgttt
     2881 taccagcggc tgggtcatcg ccagttcgcg attgaccagg ctaatcccct gcgtcagccc
     2941 ggtggtggct ttgttcaacc cgtcgcggcg agtgttgtac aacgccgttt ccgactcgat
     3001 aagttcgggg aatttattca gttcgtcagg gaagaccagc ggtttattgc tcacttccgc
     3061 ccgcagccgc gccgaggttg ccagcgccgc gtacagccgc gacatcgctt cctggacgtc
     3121 ggattccgtt ttggtgcggt taagctgcgc cagcggctgg ccctgttcga caatatcgcc
     3181 aactttcacg tcgagatgat acagaattcc gccttccagc gactgcacca cctgttcgcg
     3241 cccggaaggt tctaccgtgc cgctgccggt ggtgacttca tcaagctgga agtaccacgc
     3301 ccagccgaga aaacagagca gcatcacagc ggtaacgcgt accgccagcg ccgaacgcgg
     3361 caggcggggt tcagtcagac cacggttata ttgcagttgt ccgttcatgc cgcagacccc
     3421 ttattcttct cctgcaacac cacccgtcgc gctgtgggct cgccctgttc acgcaaaatg
     3481 gcctctttcg cgccgtccat cacaatccgc ccctgatcca tcacgattat cctatccacc
     3541 agttgcagca tcgccgtgcg gtgagtggcg ataatcagcg tgcgatgccc aatccactgt
     3601 ttcagttgtt cgattaaata cgcttctgcc atttcgtcca gtgaggcgct gggttcgtcg
     3661 agcaggacga tattcggctg agagatgagt agccgcgcca gcagcagcgt ttgccgctga
     3721 ccaccagaga ggccaaatcc cccttcctga atcatgtagt tgaggccgtt tttctgcttc
     3781 tgcacaaagg gcagcgctcc gctgagggtt aacgcgcgtt cgatatcttc atcgctggct
     3841 tccggcatcc ccatggtgag gttttcccgc agcgaaccaa agaacagtcg cccggtctgg
     3901 ctgagtagcc ccatgtctcg ccgcagatct gccggatcaa gctgcccgat actgatgtta
     3961 tccagcaaca cctgcccctg ctgggcgata cgcattccgg cgagcatttg cagtagcgtc
     4021 gatttacccg cgccgttgcg cccgagaatg gcgattttct caccagcctg aatgttgagt
     4081 ttgccaatcg ctacatcggc aatcttctct tcttcgtcgt aataaaacac cgcgttgctg
     4141 aactgataat taccgtgcag caccgcttta tgcaccagtt tgccgtgttc cggctgatcc
     4201 accgggcgct tcatcagttc atcaaggccg ttacgtgcta ctttcgcctg ctgccagcgc
     4261 gagagcacgc cggatatctg cgacagcggc gcaatggtgc gcgaagccag gatcgtcgtc
     4321 ccgaccagcg cgccggtggt catgtcgccg ttcatcacca taaagcagcc caccagcagc
     4381 accaccacgt agacgatgga ctgcacttcc tgggtccagg tgagcagcag cccggtgaga
     4441 aaacgttgct tcatgctgat ggacgaggcc acgtcgttgg tgtggttcca ctgattctga
     4501 aaacgctgtt cggcgcgcag cagtttaata tcttcaatcg actgcacggc ttccaccagc
     4561 gtagcgttgc gtaccgctga ctcgcgcatt ccttcgttcg ccagccgcgc cagcgggcgt
     4621 tgcaccagca ggccggggat caccagcaac ggcagcgcca gcaaaaccac cagcaccagc
     4681 cagccgccga tcatccacaa aataaagaca aacagcagga agaatggcag gtccgccacc
     4741 gcgccgatgg tggtggacgt aataagctcc cgcactgatt ccagttcgcg gatctgcgag
     4801 ataaacgatc cggtggattt cgatcgcacg ttgtttttca gccgtagcgc atgaccaaaa
     4861 acgcgatccg aaatacgcag gtctgcccgc tttccgatca catcagatag atgcgtacgc
     4921 accatgcgca tacagaactc aaacaggatc gccatcatca ccccggcgaa aagcacccat
     4981 agcgtcgggt acgattgcgc cggaaccacc cgatcgtaaa cctgcatcga gaaaatcatc
     5041 gccgccagcg ccagcacgtt cgccaccagt gacgccagca taatatcgcc gtagcgccgc
     5101 cagtctttta gcgaaagact ccagaaccag ttcgcctgat acgggcgaat atagtcatcc
     5161 acccgcgcgt cagggatggc gctgagaggg cgcagcagcg ccagttcgac gattttatct
     5221 tcaatatccg cgacgttaag tgtagtttcc agcccttcgt caccgctgaa ctgaacgctg
     5281 atattgtcat gattgtcgcg acgggtgatc acgccaatct gctgattatc aaacaccgcg
     5341 atcagcggca gacgccaggg gttaagcgaa tcggcggaaa actccaccaa ccgcaacccc
     5401 atccctaact gccgcgccat gtcggtgagt aactcctccc gcttactgtc gcgctcccag
     5461 ttgacggtcg cgcgcacatt ctcctgagaa aaatcgagcc gataatagcg cgccacggcg
     5521 atcatcgcct ccagccaggc ttccacggac tgggcatttt ttttcattat ctacaccacc
     5581 atcaactgat gattcgccag caaggtggcg aggtcggtgt gcacgcccgt cagcgtgacc
     5641 acgttagttg tcgcaaaagt gccgcccgtg ccgtcgcggt cgatctgcac gatggtgtcg
     5701 ctgccgctct gggtgacttt gacaaagtca ccgatgtttc cggcctgcgc atccagcgtt
     5761 gccacgccgt tgacgtagct ggctttgccg ttgccggtgt agccgctgcc ctgtaacagt
     5821 tcacgaatat caatgcgatc ggtgtcggcg gtgccttccc atgtccctac cgtaaagcca
     5881 ttcaccacgt ctgagccgtt gccgccagtg gcgtcgctgg cgttgagcag tttatagagc
     5941 aaggtgtcgt ggccgccgtt gccgatgttg agcgtgtcgt tgccgccgcg cccttccagt
     6001 tggttatcgc cgctgctgcc ggtcagaatg tcgttaaaat tcgagccgga aatgccttcg
     6061 atattggtaa atgtcgaggt gttaaaaccg gtggcctgcg cggcagtgct gcttaagtta
     6121 gccgtcacgc ccaccgtcga aagccggaaa tcaaccacgt ccatcccgcc gttcgccagc
     6181 cacgttccgg tgccggagct gtacacccag ccgccggaac cgtcgtaagt gtcggtgcct
     6241 gcggtggcga agaaggtgtc gttatagccg gtgccgacga acttgccgtt gttgctcgcg
     6301 gtacccgctt cagcgctgag atcgtaagcg tgagtggcat cggtggcctg taaaccgccc
     6361 cagctggtgt tgaccgtctg gtcgatattg ctggcggtgg tgccggtgga tttgatcagc
     6421 accgcgttgt aggtttctcc ggcattcagc ccgtagaagt tgaccagcgc cgaggtgtcg
     6481 ttaaccccca tacccgactg cgggttgatg atttgcgtgg cgaccagcac tcccgccgag
     6541 ttgtacagct tcacggtatt gccgtaatag acgttaatgc cgttgggatc ggtgatgcgc
     6601 aggtgtagcg aagtgccgta gctcaccgtg ttggtgttgc ggctcaggaa cacgctgccc
     6661 gactgtttgg acaccagcac atccaccgcg ccgtcccagt cgtagtccat tgccgccacg
     6721 ccgctggtgg taccgctctg gctgccgccg agggccgatt gcgtccagtt gctggcgttg
     6781 ctgacgttag tataaagata cgactgcccg gtctgggcga tacgggcgat atccatcagg
     6841 ccgtcgtggt tccagtccac cgccaggcta aactggctgg cgtaggtggt tgccgttgcg
     6901 cccaccgcca ccgggctacc cagtacgccg ttggtgttga ataacagcga gccgtagtta
     6961 gctcttccct ggctggcggg caggaagaga tccatatcgc cgtcaccatc gaaatcggcc
     7021 caggtcatgg aaacgctgct gctcatagcg ccactgcccg ccccgctcag gaaggtattg
     7081 gtggtgttct gcccccagac aaacgtccca ttcccctggt tgatgagcga agacagcgtg
     7141 taatagttgt tcaggttata ggtgtgctga accaggtcga tcctgccgtc attgttcaga
     7201 tcgacgccag aaccttcgtt gagcgaaaga taccccgtca ccgccccgcc caccgtggcg
     7261 ctaccgccgc tgttcgacgt ggtggagttg cctaccagcg tgcctgcgtt gttccacagg
     7321 aaggtgttgg agtccggccc gccagcgtca ccaatccaga agtcgagata gccgtcgccc
     7381 tctttatcaa atgccacaat cgagccgtac cacgtcaggg tgccgacggt gacctgaata
     7441 gcgctggaaa aggtgccatc gccgttattg gtaaagccgt taatatagcc cgtaccgtag
     7501 tcatcacggg tgataaacag atcgcccgtg ccgttacggt cgtaatcggc aatggagctg
     7561 gtggcgtagt tgttgccgct ctgatacagc gtcagcgcgg tcttcgacca ggtgagtggg
     7621 tcagtcgatt gcattacctg ctggtttgcc agcaccgtcc acatcccgtg cgagtcgagg
     7681 ccgtaggtca gcccccaggc ggtggttttg ctggcggtag tccaggtcgg tgtgtaatca
     7741 atcgccgcgt taaccgtcac cgtgccgttg ctaatattgg cgttattgcc gttacccgcc
     7801 gaacttttta cctgcgcagt aacggtatag gcggtcgcgg aaactgtcag cgcatcggta
     7861 tccggcaact gtacatacca ggtgttgtgc gccggatcga ccactaccgc gccgcccggt
     7921 tcagaggtgt aggttttgcc gttgatcacc acttcaacat actgcccact ggcaagcgcg
     7981 gcggtgatca ccccgctaat aatcggcgta gtatcgcgcg tggtctggct ggtgatttgc
     8041 gccagcgtgg tcggaataga ggtatcgacc gtcaggacaa aatcactgga ggaggtgata
     8101 ttccccgcca aatccaccac tcgcgcgctg taggtatagg caccgctcac cagcccgctg
     8161 tcgctgtagg tccagttcag agcgccgacc atcgtcaccg cccctaacag cagcccgtta
     8221 cggtagaggt aaaccacttc accgctggca agcggcgcgg aaagtacacc gttcagcagc
     8281 ggcgtagtgt cgtcggtggc ctgcgaactg cttaatgtcc cctgccgctg cccgacatca
     8341 tcggtatacg acacaatcgt tggtgtggcg gtggggttaa tcgtatcgac caccaccgtt
     8401 ttcgacacca ccgggccggt gttccccgcc agatccagca cccgcacctg ataaacgtag
     8461 ctgccgtcgg tcagcgtgcg gctgtcggtg taagtccact gtgtaccgtt ggtggtgagc
     8521 gttgtccagg tcgcgccgct atcaaggcta atctgcgcca cttcgccgct ggcaagcccc
     8581 gcccccagcg aaccggtcag cgtgagcatg gtgtcgctgg tgataaagtc agttgccgaa
     8641 ctccccgtgt cggtgctgat gctggcgatg gtgagcacct gcgccgggtt aacggtatcg
     8701 atcagcgccg actggctgct cgttgcgcca acgttgcccg ccagatcgac cacccgcacc
     8761 tgccaggtgg tggtgccgtt ggtgagggtt cgtccgtcaa cgtaactcca gttcaggctg
     8821 tctgcggcga cggtcacgtt cacccaggtt gcgccgttgt cggtactgat ttgcgcgaac
     8881 tcattagcgc cgagcgccgc gcccaatacg ccgcgcacgg taagcgtggt gtcgctggta
     8941 ataaaatcgc tgctgctggt tccggtgtca tcgctgatac cggtaatggt gatacttttc
     9001 gccgcttccg ggctggtggt gtcgaccacc acgttttgcg ttgcggtctg cccaacgttc
     9061 cccgccagat ccaccacccg caccgtgtag ttccaggtgc cgtcagtaag cgtgtgacca
     9121 tcggcatagc tccagctggt gccaacgacg gttaacgtag tccaggtgac gccgccgtcg
     9181 aggctgattt gcgcgaactc accggcagaa agcgtcgccc ccagcgtgcc gctcaccgca
     9241 agcgtcgtgt cgctggtgac aaaatcgtta gtgatcaagc ccatatcggt ggtgatcgcg
     9301 ctgatggcga tggttttcac cgcgggatct ggcgcggtag tgtcgatcac cacattctgg
     9361 ctgtcggtcg cgccaacgtt gcccgctgcg tcaatcaccc gcacctgata gagatagttg
     9421 ccatcggtca gcgtgcggct gtcgttatag cgccaggtcg tgccggtaac ggtaagcgtg
     9481 gtccaggttg tgccgccgtc gatacttatt tgcgccgtct cgttactggc aagcgcggcg
     9541 gtgagcgagc cgttgaccac taccggactg acgctggtga taaagtcgct ggcgctaagg
     9601 ccagtgtcgg cttgcaggga atcgatatta atgcccatcg ccggagcgct gagatcgacg
     9661 gtaatggtgt gattactgct accggtgttg ccaatggcat tgctcaccga ggcgttaatc
     9721 acgtaggcgt tgccgtccgc cagcgcggtg acatcggcgc tgccgagggt gtagctccag
     9781 ctaccgcccg tctgcaccgt ggtggtgtag gttttgccgt ttaaggtcag ggtcagcgtc
     9841 tgcccgacgg gcgcggtggt ggtgccgctg atcaccagcg atgagccgtg ttccgctgca
     9901 ttcaccacat cgtcgcccgc aaaggtgttg atggtgacag taggtacatc gccgttcagc
     9961 gtcacgccac ggctggcgct gccggggttt ccggcctgat cgctaaccgt cgcgctaatg
    10021 gtgtagctgc cgtcgcttaa tccggcaaac tgctgcgccg gaataaacac cgaccagctt
    10081 ccgccgctgc caaccgttgc gctccaggtt tgcccgttaa aggtaacggt caccgtctgg
    10141 cccacttcgg cggtggtggt gccgctaatg gtctgcccgg caagctgctc ggcgttgttg
    10201 ataatatcgt cacccgccac cgtggcgatg gtgaccgttg gcgcaacggt atcgacggtc
    10261 agggcgtgcg tggcctgtcc ggggttaccg gcgcgatcgt tcacgctggc ggtaatggtc
    10321 tgcgcgccct gcccgagcgc ggccagatcc accgcaggca cgttcagcgc ccaggtacca
    10381 ttggcggcaa ccgtggcggt ataggttttg ccgccaaagg tgacggtgac cgtctgcccc
    10441 acttccgcgc tggtcgagcc gttaagggtt aacggctgct gctgttcggc ggcgttgagg
    10501 cggtcgtccg tcgaaacggt gttaatgctg ataaccggcg cggtgagatc caccgccacg
    10561 ctgcgcgagg ccgtggcgct gtttccggca ctgtcctgag cggaggcgct aaccgtgtag
    10621 ctggtgccat cactgagtgc cgcaacatcc gccgcaggca cgttcacgct ccagctcccg
    10681 ttgctctgaa tggtggcgct gtagcctttg ccgttaagca acacggtcac cactgtacct
    10741 gtcgcgaaat tagtgccagt gccgctcagg gtcagcgcac tgcccgcttc ggcggcgtta
    10801 ataaggttat cgccgctgat agtgctgacc gagagcgaca ctgccgccgt gttcacctgt
    10861 acgttgtgcg tctgcgtgct gctgttgcct gcgctatcgg taatggtggc gctgatggtc
    10921 accgtgccat ccgcaaggcc ggaaatcacg ctggcaggca cgccgacgct ccagttgccg
    10981 ctggcatcgg tgctggtgac atactgctgc ccggcgatgg tcaccaccag gcggtcgccc
    11041 gccaccgcgc ccgtggcggt gccgctgata atctgcgcct gaatgtgttc gacgttgtta
    11101 atcacatcat cgcccgccac ggtgttaaaa ctgatcaccg gcgcagtggt atcgacggtc
    11161 acgcctttgc tggcgctgcc aaggttaccc gccagatcgc tgaccgtggc ggtcagggtg
    11221 tagccgttat tagcaagtgc ggttaagtcg ctggcgggca gcgttaaact ccagctgccg
    11281 tctgtctgca ccgtggtctg gtagttagtg ccgttaagcg tgacggtgag cgtctgcccg
    11341 gtttgcgcgg tgctggtgcc ggacaccgtt acgcccgccg ccgcttcgct ggcgttgatg
    11401 atgttatcgc tggcaatggt gttgatggtc accgttggcg cggcgctatc gacgctgtat
    11461 tcgtgcgttg ctgtggcgct gttgccgttg acgttagtca cccgcacctg ggcgctggca
    11521 tcgccatcac gcagggccgc caaatccgcc gccggaacgg tggtgctcca gctaccgttt
    11581 gccgccaccg tggtggtgta ggttttgccg ttgagcacaa cgctcaccac atcgcccgct
    11641 tccccgccgc tactggtgcc ggagatcacc agcgcctgcg catgttcggc atcgttgatg
    11701 atgtcatcgc ccgccacggt gttaatggtg agaaccggcg cggtggtgtc taccgccaga
    11761 ttatggctgg cgctgccggg gttgcccgct ttgtcattga ccgtggcgct gacggtgtaa
    11821 ttgcttgcgt tgagcgcgcc cagggctgaa gtgggaacgc tgacgctcca gctaccgtcc
    11881 gcctgcacag tgccggtgta ggttgcgccg ttaagggtga cggtcaccgt ctgcccggtt
    11941 tcggcggtgc tggtgccgct gatggtgagc gggcttccgg cttctgcggc gttaaggata
    12001 tcgtcgctgg cgatggtgtt aatggtgagc gtcggcgcgc tggcgtcaac gctatacgca
    12061 tgggtcgccg aggcactgtt accgctcgcg ctgctaacgc tggcctgcac attcgccgcg
    12121 ccctctggca gagttgccag atctgccgcc ggaacgttta cactccagct cccgttggcg
    12181 gcgacgcttg cagtgtaggt tttaccgcca aaagtgacgg tgacggtttg cccgctctca
    12241 atcccgctgg tactgccgga aagagtcaga ttggtgcctt tttctgcggc gttaatcacg
    12301 tcgtcgctgg caacggtgtt gatgctgatt gccaccgccg ttaaatcgac ggtgaacgga
    12361 tggctgacgc tgactgggtt attggcgctg ctttgcccgt ctacctccac ggttaacggc
    12421 cccgcaggcc aggcgctgac gtttgccgcc ggaatgccaa cgctccagct tccgtccgcc
    12481 tgcaccgtgg cggaatacgc cacactgttg atggtaaccg tcagcacagc acctgcattc
    12541 aggccgctac tgccgccggt gatcaccagc gcctgcccgt gctcgatgct gttgacgata
    12601 tcatcgcccg ccacggtatc tacgcgcagc cccggcaggt tggcatcaat ggtgatatcc
    12661 cgcgtgccgc tcccggtgtt gccattagcg ttggtgaccg aggcggtaat ggtcaaatca
    12721 ccattgccca acgcctggag atcggctgtc ggaacgctga cgctccagct taagttgctc
    12781 tgtaccgtag cggtgtaagt gttcccgccc agcgtcacgg tgaccgtatc gccagcggcg
    12841 gcgcgagtca ccaccccgct gatggtttga tcggcccccg cttcggcggc gttgataata
    12901 tcgtcgcctg ccacggtgtt gatggtgacg tccggcaggc cgctgtcgac cagtaaattc
    12961 gcctggctgc tggcggtgtt gcctgctgca ctggtggcgc tggcgctgac cgtatagttg
    13021 gcctgaccta atgccgccag atcgctcacc ggcacggtta aggtccagtt gcccgatgcg
    13081 tcggtggtgg cgctgtagtt gtgaccgttg agcgtgacgg tgacctgcgc gcccgtcgcc
    13141 agccccgtac tgccgccgct gatggtcaac ggctggcctt tttcatcggc gttcaggatg
    13201 ttatcgccgc tgatggcgtt aaaggtaatg gtcggcagcc cggtattgac cgtcacctga
    13261 tgggtagcgc tgccgctgtt tcctccggca tcggtgacgc tggcattgat ggtcaccgtg
    13321 ccattcgcca gtgccgagac gacgcttgcc ggaacgccga cgctccagtt gccgctggca
    13381 tccagcaccg tggtaaaggt atttgtgccg atagtcaccg tcacggtgct accggttgcc
    13441 gctccagtgg cggagccgct gatgatctgc gcctgggcgt gttccgtcgc gttgatcaca
    13501 tcatcgcctg ccaccgtgtt gatggtgacg accggaacgg acgtatccac cgtcaggttg
    13561 tgattaaccg aggccgggtt tccggctttg tcgctcaccg ccgcgttcac ggtgtagttg
    13621 ctggcggtca gggtgcttaa gtcggctgac ggtacgctga cgctccagct gccgtccgtc
    13681 tgtacggtgc cggtgtagtt tgtgccattg agcgtgacgg tcaccgtctg ccccgcttcc
    13741 gccgtgctgc tgccggtgat ggtcagagcc gctccggctt cggcggcgtt aagaatatca
    13801 tcgcccgcaa tggtattaat ggtcaccgtt ggcgcgctgg catcaacgct gtaagcgtgg
    13861 gtcgtggtgg cgctgttgcc gttaacattg ctgacgctgg cctgtgcgct ggcatcgcca
    13921 tcacgcagag ccgccatatc tgccgccgga accgaggtgc tccaggaacc attcgcagcc
    13981 accgtggcgc tgtaagtttt gccgccaaag gtgacggtaa ccgtttgtcc ggcttcaacg
    14041 ccagaggtgc tgccggagag cgttaacgcc gcgccttttt cggcagcgtt gatcacatca
    14101 tcggcggtaa tggcgttgat gctcaccgcc accgccgaga gatcgaccgt caccggatgc
    14161 gtaacgctta ccggatttcc ggcagaggtg ctaccgctcg ccgcaatcgt caccgatccc
    14221 gcaggccagg cgctgacatc caccgccgga acgccgacgc tccaggtgcc atccgccagc
    14281 accgccgcaa cataggtttg cccgttaatg gtcagcgtga cgttgctgcc cgccgccaga
    14341 ccggagctgc tgccggtaat caccagcgcc tgcccgtgct cgataatatt aaccacatca
    14401 tcgcccgcca cggtgtcgac ccgcagaccg gggagattcg cgtctatggt gatttcgcgc
    14461 gtgccgttac cagtattgcc tacgctgttc gtcaccgaag ccgaaatggt cagttcgccg
    14521 ttgcccaact cctgtagcgc ggaggccgga acatcgacgc tccagcttaa attcgcctgc
    14581 accgtggcag tgtaagtcgc cccgccgagc gtgacggtca ccgtgtcgcc tgccgccgca
    14641 cccgtcacct gcccgctgat ggtctgttcc acgcccgctt cggcggcgtt aataatatcg
    14701 tccgttgcca ccacgttaat ggtgacgccc ggcagcgcgg tattaacctg tacgttatgg
    14761 ctggcggaac cgctgttgcc atcggcgtca gtggcggctg cggtcaccgt gtaggtggct
    14821 tcaccgaggg cgctcacccg tgacgccgga acggtcacgc tccagttacc ggaagcatcg
    14881 gcagtggtgg tgtaattttg cccgttgagc gtaacggtaa tctgcgtgcc cgcaggctgg
    14941 ttgctggtgc cggtaatcgc cagatccgcg cctttctccg cggcgttgat gatgtcatcg
    15001 acggcaatgg tgttaatggc gagcaccgga gcgccgagcg ccacggtgac agtgtgcgag
    15061 gccgtgccgc tgttgcctgc ggagtcggtg accgtagcgg taatggtcac atcgccctgc
    15121 gccagcgcgg aaatcacgct ggcaggcacg ccgatgctcc agttgccgtt ggcgtccagc
    15181 acggtggtat aggtggtcgt gccaatcgtc acggaaaccg tattaccggt agtcgcgccc
    15241 gttgccgagc cgctgatgat ctgcgcctgt ccgtgttccg tggcgttaat gatgtcatcg
    15301 cccgccaccg tgttgatggt gacgaccgga gcggcaaggt ccaccgtcag attatgcgtt
    15361 gccgaagccg aatttctggc tttgtcgctg accgaggcgt taacggtata cgagctggcg
    15421 gtaaggctag ctaaatcgcc ggttggtacg ctgacgctcc agctcccgtc tgcctgcaca
    15481 ttgccgctgt aattaacgcc gtttagcgtt acggtcaccg tctgcccggc ttccgccgtg
    15541 ctggtgccgc tgatggtcag cgccgatccc gcttcggcgg cgttaagaat atcgtccgtg
    15601 gcgatggtgt tgatggtaac ggagggcgca gtggcatcaa cgctgtaagc atgcgttgcc
    15661 tgggtgctgt tccctgcgac attgctgacg ctggcctgga cgtttgctgc gccgtctggc
    15721 aaggttgcaa catcgacggc agggatcgtc agcccccagg tattatccgc ggcaaccgtg
    15781 gtggtgtagc ttttgccgcc gaagataacg gtgatggttt gccccgcttc cacgccggag
    15841 gtggtgccgg agagctgcaa atcagagcct ttctcggcgg cgttaatcac atcgtcagtg
    15901 gaaagggtgt tgatggtgat cgccacggca gcgagatcga ccgtgaccgg atggctaatg
    15961 ctggttgtgg ttccggcggt gttggtgcca gagacggcaa tattgaccgt ccccgcaggc
    16021 cagtttgtga catctgccac cggaacacca acgctccagc taccatccgc taaaaccgtt
    16081 gcgccgtagg tgacgctatt gataaccacc gtcaacgccg cgcccgccgc cagtccgcta
    16141 ctgctaccgg tgatcaccaa cgcctgagtg tgctcgatgc tgttaatcac atcatcgcct
    16201 gccacggtat cgacgcgcag acctggcagg ttggcatcta tcacgatatc gtgcgtcgcg
    16261 gtgccggtat tatttgcgga attggtcaac gaggcgctga tggtcagttc accgttaccc
    16321 agcgcctgca aaacgttcgc cggtacgctg acgctccagc ttaaatctga ctgcacggtg
    16381 gcgttatatt gattgccgcc aatggtgaca atcaccgtat tcccggcaac cgccgttccc
    16441 gtgacctgac cgctgatggt ctgcgccaca acgatttcag cggcgttgat aatgtcatcg
    16501 ccggagacgg tgttaatggt cagcgtcggg ccgctaagat caacggtcac atcgtgagtc
    16561 gtgctgtcgc tgttgcccgc gcgatcgctc aggctggcgg tgatggtctg gctaccggtc
    16621 gccagcgccg tgacatccgc cgccggaatg cccacgctcc agttaccgga ggcatccagc
    16681 gtggtggtgt agtttttacc gttcagcgtg acggttacca catcacccgc cgccgcgccc
    16741 gtactggtgc cgctgaccac caacgcctga ccatgttcga tggcgttgat aatgtcatcg
    16801 cccgcgacgg tgttaatggt gagatccggc gcagtgatat ctaccaccag cgcgtggtcg
    16861 gcgcttgccg ggttacccgc tttgtcgctc accgtggcgg tcacggtgta actgctggcg
    16921 gttaatccgc ttaaatccgc tgccggaaca ttcacgctcc aggtgccatc cgccagaacg
    16981 gtggtctggt acgtgttgtt attgagcgtg acggtgagcg tctgcccggc ctgcgccgtg
    17041 gtagtgccgc tgatggtgat ccccgcgcca gcttcgctca cgttaaggat atcgtcgctg
    17101 gcgatggtgt tgatggtcac aagcggcgcg gtggcatcaa cgctatacgc gcgatcggcc
    17161 tgggcgctgt tgccgttaat attactgaca ctcgcctgca cgttgcccgc gccgtccggt
    17221 agcgcagcga gatcggcagg cggcacattc accgtccagc taccgttagc ttccactgtg
    17281 gtggtgtaat ttttgccgcc aaaggtgacg gtcaccgtct gcccggcttc gacaccgctg
    17341 gtgctgccgc ttacgaccag cgtttcgcct ttttcgacgg cgttgatcac atcatcacca
    17401 gagagagtgt taatggtgat ggcgacccca gccagatcga cggtaaccgg atgggtaatg
    17461 gtggaggttg ttccggcact gttggtgccg cttaccgtga tattcaccgt acccgctggc
    17521 cagttgctga cgtctaccgc cggaacaccg aggctccacg tgccgtcggc taataccgtc
    17581 gcgccataag taacgttgtt aatctcgacc gttaacgccg ttcccgccgt taatccgctg
    17641 ctgctgccgg tgatcaccag cgcctgattg tgctcaatgc tgttaataac atcatcgccc
    17701 gccacggtat cgacccgcag gctaggcaga ttggcgtcga tcgtgatatc gcgcgagccg
    17761 ctgccggtgt tgccgacgcc attggtgacc gaggcattaa ccgtcaggtc gccgttgccc
    17821 agcgcctgaa tatccgccgc cggaacgtcc acactccagc ttaaattaga ctgcaccgtg
    17881 gcggtgtagg tgttgccgcc cagcgtaacg gtaaccgtat ccccctgcgc tgcgcccgtc
    17941 acctgcccgc tgatggtttg cgcatttccc gattcggcag cgttaataat atcgtcagtc
    18001 gccaccgcgt taatggtaac ggcaggcaag gcgctgttga ccagcacatt atgactggcg
    18061 gaattactgt tgcctgccgt atcagtgacg tttgccgtta ccgtgtagtt ggcttcaccc
    18121 aatgcgctaa ccgctgacgc tggcaccgtg gcgctccagt tgccgttgct atcggtagta
    18181 gcggtgtaat tttgcccgtt cagcgtcacc gtaatggtgg tgcccgcagg ctgattactg
    18241 gtgccggtga tttgcaggtc cgcgcctttt tccgtggctt taatcacgtc atcggtggcg
    18301 atggtgttga taccaatggt tggcgcggcg agattcacgg tgaccgtgcg gctggcgtca
    18361 tcgctgttgc ctgccgcgtc ggtaattgcc gcagtgatag tttgcgggcc gctgccaagc
    18421 gcagtgacgt cagccgccgg aacgccgacg ctccagttgc cggaagcgtc cagcatggtg
    18481 gtgtaggttt tactgtttag tgtgacggtg atgacatcac ccgcttcgcc gccagtgctg
    18541 gagccggaga tcaccagcgc ctgtccgtgt tcggcggcgt taataatgtc atcgccggag
    18601 acggtgttga tggtcagcac cggcacggtg agatccaccg ccagcccgtg gttagcggac
    18661 gccgggttac ccgctttatc gcttaccgag gcactaacgg tgtactggct ggcggtcaga
    18721 ttgctgagat ccgccgtcgg taagctgacg ctccagctgc cgtccgcctg gacggagccg
    18781 ctgtaagtca caccattaag cgtgacggtt accgtctgcc ccgcttcggc ggtgctgcta
    18841 ccgctgatgg ttaacggatt gcccgcctcg gcagcgttca gaatatcgtc ggtggcgatg
    18901 gtgttaatgg caagcgtcgg ggccgtggca tcgacgctgt aggcgtgggt tgccgaagcc
    18961 gtgttgccgt taatagtgct gacgctggcc tgcacggtgg cgtcgccgtc gcgtaacacg
    19021 ctgagatcgg cggcgggtac ggtggttgtc cagctaccat cgcccgctac ggtagcggtg
    19081 taggttttcc cgccaaaggt aacggtgacc gtttgcccca cttccacgcc ggaggtgctg
    19141 ccggaaaggg ttaaatctgc ccctttttcg gcggcgttaa tcacatcgtc gccggaaacg
    19201 gtgttaatgg aaatcgccac cgccgccaga tcgacggtga ccggatgggt gatggtggat
    19261 gttgttccgg ctgtgttagt gccacttacc gtgatattca ccgtacccgc aggccagtta
    19321 ctcacgtctg ccgccggaac accaacgctc catgtgccat cggctaatac tgttgcagcg
    19381 taagtgaccg tgttaatcac caccgtcagc gccgcgcccg ccgccagccc gctgctgcta
    19441 ccagttatca ccagcgcctg agcgtgctcg atgctattga ccacatcatc gcccgccacg
    19501 gtgtcaacgc gcagacctgg caggttggcg tcgatagtaa tatctcgcga accgctgcca
    19561 gtattgccaa cgccgttggt caccgaagcg ttaaccgtca gattgccatt gccgatggcc
    19621 tgaatatccg ccgccggaac gtccacgctc cagcttaaat tcccctgcac ggtagcggtg
    19681 taagttttcc cgccaagcgt tacggtaacc gtatcgcccg ccgccgcccc cgtcacctgg
    19741 ccgctgatgg tttgcgcatt gcccgattcg gcggcgttaa taatatcgtc ggtcgcaact
    19801 gggttaatgg tgacgccagg cagcgcggta ttgacctgca cgttatggct ggcggaattg
    19861 ctgttgcctg cgctgtcggt gacgttcgcc gtcaccgtgt agctggcttc gcccagcgca
    19921 cctactgcgg aagctggcac cgtagtgctc cagttgcctg cagcgtccgt cgtcgcggtg
    19981 taattttgcc cgttaagcgc cagcgtaatc gtggtgcctg ctggctgatt actggtgccg
    20041 gaaatttgca gatccgcgcc tttttcgctg gcgttaatta tgtcatcggt ggcgatggtg
    20101 ttgatgtcaa tggttggtgc ggtgaggtta acggtgaccg tgtgtgtcta attgtcgctg
    20161 ttgcctgccg catcggtaac cgtggcggtg acagtttgcg ggccgctgcc aagcgccgtg
    20221 acatccgccg ccggaacgcc gacgctccag ttgccggagg cgtccagggt ggtggtgtag
    20281 gttttactgt ttagcgtgac ggtgacgaca tcccccgctt ctccgccggt gctggaaccg
    20341 gaaatcacca gcgcctgccc gtgttcggtg gcgttaataa tgtcatcgcc cgcaatggtg
    20401 ttgatggtca gcaccggaac ggtgagatcc accgccagat tatgcgtggt gctcgccgga
    20461 ttgcccgctt tgtcattgac cgtggcggtc agggtgtggc tgccatcggt caatgcctca
    20521 agatctgccg ccggaatatt gatgctccag ctgccgtccg cctgcaccgt tgcctgatag
    20581 gtctgcacgg tcgggctgtt aagcgttatc gtaacaatct gccccgcttc ggcggtggta
    20641 ctgccgctca ccgtcacgcc cgcatcggct tcgctggcgt tgacgatatt gtcgctggcg
    20701 acggtattga taatgatggt tggcgcgctg ctgtcgacgc tgtagttgtg caccgccgag
    20761 gcgctattgc cgttgatatt gctgacgctg gccagtgcgg aggcgctgcc ctcgggtaat
    20821 gacgccagat cggcggcggg tacggtggca gtccagctac catcgctcgc tacgctggca
    20881 gtgtaatttt tcccgccaaa ggtgacggtg acggtttgac cgggttctac gttagtggtg
    20941 gtgccggaaa gggttaaatc agcgcctttt tctgcggcgt taatcacatc gtccgtggcg
    21001 atggtgttga tggtgatcgc tgccggagtg agatccaccg tcaccggatg cgtaatgctc
    21061 accgagtttc cggcgctact ttcccctgaa acggcaatat taaccgtccc cgcaggccag
    21121 gcgctaacct gcgccgccgt gacgcccacg ctccagctac catcggcctg caccgcagtg
    21181 gtgtattcca cattattaat cgtaacggta agcggcgtgc tttcagccag ccccgagctg
    21241 ctgccggtga ccaccagcgc ctgcccgtgc tcgatgatat tgaccacatc atcgcccgcc
    21301 accgtatcga cccgcaggcc gggcagattg gcgtcgatgg tgatatcccg cgtgccgctg
    21361 ccggtgttgc cgttttgatt ggtgactgag gcattaaccg ttaaatcgcc atttcccagc
    21421 gcctgaatat ctgccgctgg aacgtccacg ctccaggtga gattgctgcc caccgtcgcc
    21481 gtataagtat taccacccaa cgtaatagtt accgtgtcgc catcttccgc gccagtcacc
    21541 tgcccgctga tggtttgcgc cacgcccgct tcggcggcgt taataatatc gtcggttgcc
    21601 accggattaa tggtcacacc gggcagcgcg ctgtcgacca gcacgttatg gctggcagtg
    21661 gcgctgttgc cgatatcgct ggtcaccgcc gccgttaccg tatagttggc ctggcctaat
    21721 gctgtaaccg ccgatgccgg aacggtgacg ctccagttgc cggaggcgtc ggtcgtggtg
    21781 gtgtaatttt gcccgttcag cgtcacggtg atggcggtgt taacaggctg atcgctggtg
    21841 ccggtaatct ggagatcggc ccctttttcg ctggcgttaa tcacatcatc gcccgcaatg
    21901 ctgttaatgc cgataagggg cgcggcggta ttgaccgtga cggtcaatga ctggctgccc
    21961 gtgtttccgg ctttgtcggt taccgagacg ctgacaggat aactgccgtc cgccagccca
    22021 ctgaccaccg aggccggaac gcccagactc cagttgccgg aaccatccac caccgtggtg
    22081 taatccacat tattcagcgt cacggtgacg atatcgcccg cctgagcgcc agtaaccgtg
    22141 ccgctgatga tttgcgcctg agcatgttcc ggcgtgttga tgacgttatc ggtcgccacc
    22201 ggattaatgg ttaccaccgg cggcgtggca tccaccagcg ccacgcgtga aacgctgctc
    22261 ggattaccgg cgacatcgtt cacgctggcg gtgaccgtag cgttgccgtc agccagtgcg
    22321 cccacgttgg cggcgggcac agtcacgctc caggtgccgt ctggctgcac gacgccctga
    22381 tacgtctgcc catttaacgt aacggtcacc gtctgccccg gctgagcgtc ggtggtgccg
    22441 gtgatgggca gatcctgccc gctttcggcg gcgttaatga tgttatcagc ggtcagtgga
    22501 tcaatgctta aggccggggc ctggctatcg acggtaatgg tgcgggaagt gttgccggta
    22561 ttgccgctga gatccgtcac agaagcggta atcgccaccg cgccgtcagg cagatttccc
    22621 atcgcggcgg caggtaccgt cagactccag gagccatcgg cctgcacggt agtggtaaaa
    22681 ctctgtccag ccagggttac tgtgacggtt tgtcctgtct cagcctgttg agtgctaccg
    22741 gtaagggtca gtggcgcacc tttttctgcg ccactgataa tgtcatcgcc ggagacggtg
    22801 ttaatggtga tcaacggaga aacaccgacc agcgtgattg ctcgagacgc cgcgccagta
    22861 tttcctgcag catctgtcac ggtgacgaaa atcgtattgg ctccgcgatc cagcgtgcgg
    22921 gttaccgctg ggtccagcgc cacattccag ctgccatctg ccagcacgat gcccgtcagg
    22981 acatgagtgc ccaatttcac cgttaccgta tcgcctggag aagcaccgct gacttgtccg
    23041 gagatgatct gcgcgacggc ctgctcggca ttattaagaa tatcgtcgcc cgccacggta
    23101 ttaacactga ccacgggtgc tgaggtgtct accgtgaaat tcgcgctgtg gctggtggtg
    23161 ttccctgccc gatcgctgac actggcattg acagcgtaat taccctccgc cagtgcctgg
    23221 acttccgtgg caggcagcgt cacttgccag gttccgtcgc taccgacggt tgctgtatgg
    23281 cttttcccgt tgacggtcag tgttacggtt tgccccgctt ctgcattcga cgtgccgctc
    23341 agtaccagcg cgacgttatg ttccgcagca ctgataatgt tgtcctgagc gatggtgttg
    23401 atggcaagcg ttggcggttg ggtatcgacc gttagtagct gcgcgccggt gaccgtattg
    23461 cctgtggcgt ctttcccact gaccagcacc gcctgattcc cttcgcctaa cgcctgtgca
    23521 tcggcggtcg gcacctgcac gctccaggca ccgttagcgc ctacgctggt cgtgtaggtt
    23581 ttgccgttta gcgtaacggt gagttctgtt ccctgcgcca gatggctact ggtgccagaa
    23641 agcgtaaacc cggcggcctg ttcgctggcg ttaataatgt tatcgcctgc ggtggtggca
    23701 acctgcacgc tggcctgact attgtcgatg gttaacgtta tcgaaccggc gtcagtgatg
    23761 gtactgccga caatttgcac cactgaccag gtatgttcac ctgcactttg cgtgggtaac
    23821 gagacactcc agtgaccgtt gttgtctacc tgagtactgg caatagtatt gccgttactg
    23881 tctttaatct gaatagtcgc ccccgcctgc ccgctgccgc taaaggttgg cgtggtgtca
    23941 tcggtgatgt cattggtggc tagaatgccg cgctgatcgc cctgattatc ggtaactaaa
    24001 aatgatggtg tggcgttgcc aggctcagca ggtggcgtag ggttattaat cacttctgtt
    24061 ttcgagtcgc catcgccacc gcttgccagc agtgcaccaa gcgcgccacc acctaccgcc
    24121 gcccccgcca gccaacccca cgggaaggcg ctggtctgag caacggtatc aagaaatggc
    24181 gcaatgcttt caatcgcagt ggtctgggca gtcagttcta cgggggctaa tccacccgca
    24241 gcagtatcgg caaaggtgat atgggttagc tgttgaccat cggcaaacac cagttccgat
    24301 tgttcagctg tattcgccgc ttgcaggaaa taaccgttgc agcggatcac cgtgccgtcc
    24361 tgcatataga tcagcaggtc atttccttca cggacgtaac gcgcaacggc agaggcctga
    24421 gcatgaacct gcaccacgga aggctcataa ataacaaccg tgacgtcgcc aggattaatc
    24481 agcgtttgtt tgacggatgt tttacgcgaa ataacatcaa taatcaggct cataatggca
    24541 ttcctgataa atggcgcaca tccttagcga gtccatttat ctaaataaga caaaggtaat
    24601 cacttaacaa cggcccacta ttaataatgg caggattatt tcaagtcggg taagtcgttg
    24661 tataagccct gtttaattcc cgctaatgga ataaggttat tcaccgcagc ggcataattt
    24721 acggcggcga cccagccatc ataattggca tttatttcag ccgattgcgc ctgaaagaca
    24781 tcttgttcga cggttaatag atcattcaga ctgcgtttac ccaatttata ttcattttgg
    24841 tacacatctc gcgttttgtg cgcactttcg gattgcgcta aaccggcttc ttcacgaccc
    24901 cgtgcgccgg tccagttcgc atatgccaca gacgctcgtt gcagcacatc cagttttgcc
    24961 tgttcgacct gcgaggcaga gattttttgt tgcccttcgg cctgttgcac ctgggcagaa
    25021 accgcgccgc cctgatacag cggcgcgtta acgttcagtt gtagctgatc atcccaatac
    25081 gagcggtcgc tggtctggta gcgcgtttta cccccctgaa tactgagcgt tggccagtat
    25141 tgcgctttcg ttttttccac gccgtactgt gctgactggc gtaagttttc tgccgccagc
    25201 accagcggga tggactggta atcaatattc ttcagcgata ccggctgctc ggctaattca
    25261 gcaggtggcg cggctatcgc ctccggctgt acgccagtga gcaccgccag ttgcgctttg
    25321 gcgctcgcca tctgcgcctg atattgctcc agcgttgagc gcattccggc aatacgcgtc
    25381 tgggcctgta attcatccga cgatgagttc aggcctgcgt tagcgcgcaa tgccgccatg
    25441 ttgtagacgt tttccagcga gtgaatattg cgttgagccg catcgcacaa agcctgataa
    25501 cgactgactt ccatgtaggc agtcgccgtt ttctctgcaa catcggttaa ggttgccatc
    25561 aatttaaagc ggtagctgtc acgggcggca gtttgcagat tgatatcgtt gttagtttta
    25621 ccaaagtcat acaccagttg tgtcagggtt atgccatacg aaacgttgtt atcaagcctg
    25681 ccgctggagt ccgtttgtcg ggacggcccc gcgttgccgg ttaatccaac gtgcggatac
    25741 caggcacttt tggcttcatc aatttgcgct tcgccaatac ctatctgcgc cgcctgttga
    25801 gtgacaccgg gatttcgcgc aaaggcgcgt aatatacttt cctgtaaaga taattgggca
    25861 actggcgtta ccgctggggc attcgcccat tgtggcagcg gtgcagcaat tgcagcttct
    25921 cccatcacca taaaacttat aaaaaaaata agtccattat ttttacggtt tttaaactgg
    25981 aatcgcgcca tagataagtc acttccttcc aggtcattat tttaactggt ttttataacc
    26041 agtctcgttt tattaagaag ggtaataact tgtgcatctc ttttgtcgat cattgatatg
    26101 ggcaaagaaa tgcagctaaa aagcttatac gttatttatt tagaatacac tacgactttt
    26161 agattaccat catacacatt aaaaataatc cacggtaata agtgattagc atcacagatt
    26221 tatcgcgtat atatcatttt tttgtgactt atttattaaa ggaataagaa aggtaatttg
    26281 aattaaagac aaaggcgcag tacaggcagt actgcgcgca gagaggtgtt atacggtttt
    26341 attgcgcttc atgaccattg ccacaataag gctgagtaag attgcaccaa taatccatat
    26401 caacgcacgg ctgccctggg accacaggct gtagataaag ccaacgatca ccaacaacat
    26461 caccaacgta cctacgataa gaatcggcaa cgaggcgtga atatcatgac gcaggcggat
    26521 cgccaccaca aacaccgcca gatagcaaat caaaaaggtg gcgctggcaa cgctggcgag
    26581 tgagcctaaa tttagtgccg ccgtcatcag cataatcaac accacgacaa tgatgttgcc
    26641 ccaggtactc tgccgccaca gggatttatt cattagcttc ggcagttcgc gttcgctgcc
    26701 catgttgtcc atgatgttaa acacggcgaa caggttcgcg ttgatggctg aagccgtcgc
    26761 cagtaaagcg ccgatgacga cgatcacata acccacatgc ccgagcagcg gagaagcagc
    26821 ctgcgctacg gcggtatcgg catatttttc taactctaat gccgatacat cgctaagcaa
    26881 aaccagcgcc agcgagatat aaagcaacgt ggtaacgcca attgccacca gaaacgcccg
    26941 tggcataatg acctgcggat ctttcacttt atccgccgcg ttcgccatca tgccaaagcc
    27001 cgcataggca aggaaggtta tcccgataca ggagaagaac gcaccggagc tggggggcgc
    27061 agagacggaa atatgcgccg gttgcagcga ccagacaccg gcaataatca gcaataacag
    27121 gatcatcatt ttaatgccga cgaggatcac ctccagccgc cctaccgcat ggttgcttaa
    27181 ggagttgaaa agcgtcatca ccgcaatgat ccccaacgcg tagagcaaaa taaggtgctc
    27241 ctcctggctg ccttcatgca aaaactgcac ggcataagcg ccaaaagcac gggcgaccat
    27301 ggcgatgctc accgccagcg tcaacaggta cagtaacgag agcgccagcg aaaagacgcc
    27361 gttgcctaat ccgcgacgaa agaagtcgat aatgccgcca ttgctgggat agctcgcccc
    27421 cagacgcgca taggcataac cggaaaacat cgccacaata ccgccaaaag caaaggcgac
    27481 ccaggtcgag gcttccatta gcaacgcagc ctgccccagc agcgcgaaga tccccgcccc
    27541 caccattgcc ccaatgccga tggaaacgac gttccataga ccgaggggtt tgttaccgtt
    27601 attaccttcc gtgttcatca tgatcagccc ttaaacacgt tatagccgag ttgcttagcg
    27661 accgatgcca ccattttttg accgcgaaca ctgttgcctt cttcgtccag cggtggtgag
    27721 aacgcggcaa ttcccatcac tccagggacg accgccagaa taccgccacc tacaccgctt
    27781 ttgcccggta aaccaacacg atacgcccag tcaccggagc gaccatacag cccttccatc
    27841 atcatttcgg ccagaatgta cggcacgttg tcggcctgaa gaacgcgttt atgcgtcaac
    27901 ggattcacac cacccgccgc cagcgtcgcg ccaagcgttg ccagttcaac agtattgatg
    27961 agcgtggagc actgacgggt gtacacgtca caggcttcca ttgcatcaca atagagatat
    28021 ccggcggagt acagcagcca ggcaatggcc cggttatgga agttggttgt ttgttccgac
    28081 tggttgactt cgtcagagag cgctaccagc tcaccagcca gttgctgttg gatatgtaaa
    28141 attcgctgcc agcgttgttc ggcattttca gcgttaatca ggctggtggt ggcaatagcg
    28201 ccagcattta ccagtggcga cagcggtttg ccgccatgca actctaaggc gataactgag
    28261 ttaaagggca atccggtcgg gtcagcgcca attttgtcct gtaccgcctg cgggccgaca
    28321 tcttctaacg caagggctaa cgtacagact ttcgagatgg attccagcgc aaagcggtaa
    28381 tcactgtcac ccgcactata gacgttgcca tcgctggtca cgatagccac tgctgccagt
    28441 tgaccaggca cattcgccag aaagggaatg taatcggcat tttgtccgcc gttaagtgag
    28501 tgaaattggg tgtaagcctg atccactgcc tgctgtaatt tgtttgcatc taacatcttt
    28561 tgttaactcc tttttataga tgcgggaggt aattcctcac cccggtgccg attttcaggc
    28621 atcctgattt aacttagcac ccgcaactta actacaggaa aacaaagaga taaatgtcta
    28681 atcctgatgc aaatcgagcc gattttttaa tctttacgga cttttactcg cctgggttat
    28741 taatttcttg accttcccct tgctggaagg tttaaccttt atcacagcca gtcaaaactg
    28801 tcttaaagga gtgttttatg tcacaaacta tcgacctgac cctggacggc ctgtcctgcg
    28861 gtcactgcgt taaacgcgtg aaagaaagtc tcgaacagcg cccggatgtg gaacaagcgg
    28921 atgtgtctat cactgaagcg cacgttaccg ggactgccag tgcagaacag ctgatcgaaa
    28981 ccatcaaaca agcgggttat gacgcatctg taagccaccc aaaggctaaa ccgctggcgg
    29041 agtcatcaat cccgtcggaa gcactgacag cggtttctga ggcgcttccg gcagcgaccg
    29101 ccgatgacga tgacagccag cagttgctgc tgagcggcat gagctgcgcc agctgtgtca
    29161 cccgcgtaca aaatgcgctg caaagcgtac cgggcgtcac tcaggcacgg gtaaacctgg
    29221 cggagcgtac tgcgctggtg atgggcagtg cctccccaca agatttagtg caggcggtgg
    29281 aaaaagcggg ctacggcgcg gaagcgattg aagatgacgc gaaacgccgc gagcgccagc
    29341 aagaaaccgc cgtcgctacg atgaagcgct tccgctggca ggcaattgtt gcactggctg
    29401 tgggtatccc ggtaatggtc tgggggatga tcggcgataa catgatggtc accgctgaca
    29461 accgcagcct gtggttggtt atcggcctga taaccctggc agtgatggtt ttcgccggcg
    29521 gccattttta ccgcagtgca tggaaaagcc tgctgaacgg tgcggcgacg atggatacgc
    29581 tggtggcgct gggtacgggt gtggcgtggc tctattcgat gagcgtcaac ctgtggccgc
    29641 agtggttccc gatggaagcg cggcatcttt actatgaagc cagtgcgatg attatcggtc
    29701 tgattaatct cggtcatatg ctggaagcgc gcgcacgtca gcgttcttca aaggcgctgg
    29761 agaaattact cgatttaact ccgccgacgg cgcgcctggt tactgacgaa ggtgaaaaaa
    29821 gcgtgccgct ggcggaagtt cagccaggca tgctactgcg cctgacgacg ggcgaccgcg
    29881 tgccagtaga tggcgagatt acccagggcg aagcatggct ggatgaagcg atgctgacgg
    29941 gcgaacctat cccgcagcaa aaaggcgaag gcgatagcgt ccatgccggg acagtggtac
    30001 aggacggtag cgttctgttt cgcgccagtg cggttggcag ccataccacg ctatcaagga
    30061 ttattcgtat ggtgcgccag gcccagagca gcaagccaga aatcggtcag ctggcggata
    30121 aaatctcagc cgtatttgtg ccggtagtag tggttattgc gcttgtcagt gcggcaatct
    30181 ggtatttctt tggtccggca ccgcagattg tctataccct ggtgattgcc accacggtgc
    30241 tgattattgc ctgtccgtgt gcgctggggc tggcgacgcc gatgtcgatt atttccggcg
    30301 tcgggcgggc ggctgagttt ggcgtgctgg tgcgggacgc cgacgcgctg caacgcgcta
    30361 gtacactcga taccgtagtg ttcgataaaa ccgggacgct gaccgaaggg aagccgcagg
    30421 ttgtcgcagt gaaaacattt gctgattttg acgaagcgca ggcgctgcgt ctggcggcgg
    30481 cactggaaca aggttccagc catccgctgg cgcgggcgat cctcgataaa gcaagcgata
    30541 tgcagctgcc gcaggtcaac ggtttccgca cattgcgcgg gctgggcgtg agcggtgaag
    30601 ctgaaggtca tgcgttattg ctgggcaatc aggcgctgtt aaatgaccag caagttgata
    30661 ctaaagcgat tgaagcagat atttcagctc aggcatcgca aggggcaacg cctgtgctgc
    30721 tggcggttga cgggaaagcg gtagccctgc tggctgtacg cgatccgttg cgtagtgata
    30781 gcgtggcggc gctgcaacgt ctgcataaag cgggatatcg tctggtgatg ttgaccgggg
    30841 ataacccaac caccgccaat gcgatcgcca aagaagcagg gattgatgag gtgatcgccg
    30901 gggtgctgcc ggacggtaaa gccgaagcga ttaaacgcct gcaaagtgaa ggacgtcagg
    30961 tggcaatggt gggcgacggc attaacgatg cgccagcgct ggcccaggcg gatgtcggca
    31021 ttgcaatggg cggcggcagt gatgttgcca ttgaaaccgc ggcgattacc ctgatgcgcc
    31081 atagcctgat gggcgttgcg gatgcgctcg ctatttcccg cgcaacgctg cgcaacatga
    31141 agcagaacct gctcggcgcg tttatctaca acagtatcgg tattccggtc gccgccggta
    31201 ttttgtggcc gtttactgga acactgctta acccggtagt tgccggagcg gcaatggcgc
    31261 tctcgtcgat taccgtagtg agtaacgcca accggttgct gcggtttaaa ccgaaggaat
    31321 aagtacgtca ttgcggatgc gctgctttag ggtggcgcat ttggcatcga cgcatgactg
    31381 ccggatgcgg cgtaaacgcc ttatccggcc tacggttcag gcacaggttt gtaggcctga
    31441 taagacgcgt aagcgtcgca tcaggcaatg gtgctcggat gccggatgcg gcgtgaacgc
    31501 cttatccggc ctacgagatg aagcgatgtt ttggccttac gcgacctttt ttgcacgctc
    31561 ttcacggcgt gccaaatatt cagccactgt ttcaatccgt cccaattctt cctgggtgcg
    31621 catgttgttt tcaacttccc aaagatcaac tgccgcctgg aggtttagcc aaaaatcgac
    31681 tgtggtatca aaaactttcg ccagacggaa tgccatctca gtggtgagtt tacgattgtt
    31741 attgatcagt gcactgacgc tattacgatg aacatgcagc aactctgcta actcattgat
    31801 tttcaaatct agcggttcca gatattcata gagaagaata tctccgggtg tcgtcggttt
    31861 tcttgttgcc tgtttcatag tttttaactg ttgcttccct gtaaatagcg atacaagaac
    31921 atactgaatc atacaaacac attaaacaag tccccaactc catttgcgcc atccctcccc
    31981 gcgcgctagc atgatatttc acaaagggag gtcgtatgga tctgttgtac cgggtaaaaa
    32041 cgctttgggc cgcgctgcgc ggtaatcatt acacctggcc tgccattgat atctctcttc
    32101 ccggcaatcg ccattttcat ctgattggca gtattcatat gggtagccac gatatggctc
    32161 ccctacccac ccgtttgctc aaaaagctca aaaacgccga tgcgctgatc gtcgaggcgg
    32221 atgtttccac cagcgatacg ccttttgcta atttgcctgc ctgcgaggcg ctggaagagc
    32281 gcattagcga ggagcaactc caaaaactgc agcacattag ccaggagatg ggcatttctc
    32341 cctcactctt ttctacccaa ccgctgtggc aaatcgcgat ggttcttcag gcgacgcagg
    32401 cacaaaaact ggggctgcgg gcagaatacg gtatcgatta tcagctattg caggcggcga
    32461 agcaacaaca taaacccgtg attgaactgg aaggggcgga aaaccagatt gccatgttgc
    32521 tccagctccc tgacaaagga ctggcgctgc tggacgatac gctgacccac tggcatacca
    32581 acgcacggtt gctgcaacaa atgatgagct ggtggctgaa tgcgccgccg caaaataatg
    32641 aaataacgct gcccaatacg ttcagtcagt cgctgtacga tgtgctgatg catcagcgaa
    32701 atctcgcctg gcgggataaa ttacgcgcca tgccgccggg gcgatatgtg gtcgcggtcg
    32761 gtgcactaca cctgtatgga gaagggaatt taccgcaaat gttgcgctaa aaaaatggcc
    32821 aatatcgcta ttggcccgtc aaagaggaat ttcatttttt tattattatg ccgtcacttt
    32881 aagcgacggt gtaactcgat tgtcgctcaa tctcaccatc ctgccaatac tcttaggcaa
    32941 gatgactctt ttttttggac cgccatcagg cgtatgcttg cttcgttttt ttgtttaagg
    33001 caggaaaatt accatgacac ccgcagttaa atcactcgaa aaaaacaaga tttcgtttca
    33061 aatccatacc tacgagcacg atccggctga aaccaatttt ggcgatgaag tcgtcaaaaa
    33121 attaggtttg aatccggatc aggtctacaa aacgctgctg gtcgcagtga acggtgatat
    33181 gaaacacctt gccgtggccg ttacgccggt cgcaggtcaa ctggacctta aaaaagtagc
    33241 aaaagcgctg ggtgccaaga aagttgagat ggccgatccg atggtcgcgc agcgttcgac
    33301 gggatacctg gttgggggga ttagcccact ggggcagaaa aaacgtctgc caacgattat
    33361 cgacgccccc gcacaagaat ttgccactat ttatgtttcc ggcggcaagc gcggactgga
    33421 tatcgaactg gcggcaggcg atctggcgaa gatcctcgat gccaaatttg ctgatatcgc
    33481 ccgccgcgat taagcattgt gccggatgcg aacatccggc actttcggat tactgccagc
    33541 tcacctcacc tttcggttca taaacactca catccagcgg cgagcttttc tggatatacg
    33601 ctttcagcac ttcggcatca ataaagccgg tattcacata gcccggtttg ttatcaaggc
    33661 gcggatatcc atcaccgccg gtggcattga agtttaatgt cgccatacgg taagttttcg
    33721 ctggatcgac cggttcgcct ttgattttaa ggtcgttcag tttgccgtct ttcgccacaa
    33781 agctaacgtt ggcaaattgc gggtaggcac ctgaatctgg cttcatctgc gcgacggcgg
    33841 tcaggtaatc aatcacctct ttaccggtca tgtcggcata caccaccaca ttgccgaatg
    33901 gctgcacttt cagcacgttt ttatagctga tatcgcctgc ttcgatagaa tcacgaattc
    33961 cgcctccgct catcaccgca aagtcggcac cagtgcgatc catttgtgct gccagaatca
    34021 accgccccat attggtctgt acaaaacgca ctttgtcacg atcgccttcc agacgaccat
    34081 tggtttcgcc tattttcact tccagctgcg ctttgccttt gttctggaac ggcgataaca
    34141 gcgagatcat ttgctggttt tcagcgattt caggggtgta cagcacgcgc tcgcttttcc
    34201 cgtcttccca ggtcactttc ttcttcaggt tcaccggaat cagctggtag ttaaccattt
    34261 tcatttcgcc gttgcgaaac tcaaagtcgg cccgaccaac gtacttgccc cactcatgcg
    34321 cctgtacaat ccagatgccg ttttgttgat ccggtttgca tggcgtacct ggaacgtaat
    34381 ctacctgctt tttattttcc gctgccatac agactggatc ttgcgagtga ccgccgacaa
    34441 tcatcgccag cgatccggca ggcaacgcgc gtgccatctc cacatcgccc ggtgcgttag
    34501 aaccgtgatc gccgttatcg taatgcccca tatgtgtcgc cgcgataata atgtctggct
    34561 tttctgtctg ttgcagctcc tgaatcacca gcttcgcttc atcggcgggc ttacggaact
    34621 caatatcggt gaagttttct ggattgccta acttcgctgt gtcatccgtc gtcagaccaa
    34681 taacggcaat tttcagatcc tgacgcttaa acagcgccca tggtttaaac aggcgctcgc
    34741 cggtactttt ctggtagata ttggcagaaa gtaacgggaa ctttgcccac ttttcctgct
    34801 ggcgtaatac ggtgagcggg ttatcaaatt catgattacc gatcgccatc gcgtcatagc
    34861 ccaccagatt cataccgcga aaatcaggtt cggcatcctg taagtcagac tcgggcacgc
    34921 cagtgttaat gtcgccaccg gaaagtagca gcacgctacc gccttcagcc gcaacctctt
    34981 tgcggatacc atccaccagc gttttttgcg ccgccagacc atattcgcca tattcattgc
    35041 gccaaaaatg cccatgatga tcattggtat gcagaactgt aattttgtag gttttatcct
    35101 gctcatacgc cagagcagct tcactcgcca gtgtaaatgt ggttaacagt gctaacgcca
    35161 cgccccgctg caataatttc atacttctct ccctgacctg atttcaacgt gttgaaaaat
    35221 agtatgacgc gacatcatag acaagtctgc gtccggtgaa gataaatctg catccagatt
    35281 agaaattctg tattcagcgt tgagacttcc ttcaggcggc gcatacaggt atgttagtcg
    35341 ggtaataatc acaaataaga tcattcttaa catcgcaacc ctgaaaaagt gactttccta
    35401 tggcaatgag tgaacaaacc cagcctgtgg cgggcgcggc tgcgtcaacg accaaggccc
    35461 gaacatcgtt tggtatttta ggtgctatca gcctctcaca tctgctgaac gacatgatcc
    35521 aatcgctgat tctggcgatt tatcctctgc ttcagtcaga attttctctg acatttatgc
    35581 agattggcat gataaccctc accttccagc tcgcctcttc gctactgcaa ccagtggtcg
    35641 gctactggac cgataaatat ccgatgccgt ggtcgttgcc aattggcatg tgctttacct
    35701 taagtggtct ggtgctgctt gcgctggcgg gcagttttgg cgcagttctg ctggcggcgg
    35761 cgctggtcgg taccggttca tcggtctttc atccggaatc ttctcgcgtg gcccgtatgg
    35821 cttccggcgg gcggcatggc ctggcacaat ctatctttca ggtcggcggc aactttggca
    35881 gttccctggg acccttgctg gcggcggtga ttatcgcgcc ttatggcaaa ggcaacgttg
    35941 cctggtttgt gcttgcggca ctgctggcga tcgtggtgtt ggcgcaaatc agccgttggt
    36001 actcggcaca gcaccgaatg aataaaggaa aacccaaagc gacgattatc aatccactgc
    36061 cgcgcaacaa agtggtactg gcggtcagca ttctgttaat cctcattttc tcgaaatatt
    36121 tctatatggc gagcatcagc agctattaca ccttttatct gatgcaaaaa ttcggattat
    36181 ctatccagaa tgctcagctt catctgtttg ccttcctgtt tgccgttgcg gcaggtacgg
    36241 tgatcggcgg gcctgtaggg gataaaattg ggcggaaata tgtgatttgg ggctctatcc
    36301 tcggcgttgc gccgtttacg ctgattttac cctacgccag cctgcactgg acgggggttt
    36361 taacggtgat tattggattt atcctcgctt cggcattctc tgccattctg gtctacgctc
    36421 aggagctgct tccaggacgt atcggtatgg tttctggact ctttttcggt tttgctttcg
    36481 gcatgggagg tctgggagcg gcagttctgg ggcttatcgc cgatcacacc agcatcgagt
    36541 tagtctataa aatatgtgct ttcctgccac tattggggat gttgaccata ttcctgcctg
    36601 ataaccggca taaagactga tttcattgca gccaaaggca aactttggct gcatcgtttt
    36661 acagtcgcca taagcctttc ctctgttaaa ccgccttctg ttttccgatc cgtgcttttt
    36721 tgcagctaat tccatttttc tgcaaaattc aataattatt catgaacatc atcaatgaaa
    36781 ttgtcataaa ctattacctg taattttggt tttcccggcc aaaaatggac aataaccacc
    36841 aggaaaagga gacggaatgc atcacgccac cccgcttatc accaccattg ttggcggcct
    36901 tgtgctcgcc tttatcctcg gcatgctggc caataaacta cgtatttctc ctctggtggg
    36961 atatctgtta gcgggtgtgc tggcaggacc attcactccg ggctttgttg ccgataccaa
    37021 gcttgcgccg gaactggctg aactgggcgt cattctgttg atgtttggcg tgggtctgca
    37081 cttttcgctc aaggatttga tggcggtaaa ggccatcgcc attcccggtg cgatcgccca
    37141 gatagccgtg gcgacgctgc tgggtatggc gctctctgcc gtgctgggct ggtcgttaat
    37201 gaccggtatc gtgttcggtt tatgtctttc caccgccagt accgtggtgt tactgcgcgc
    37261 acttgaagaa cggcaattaa ttgacagtca gcgtgggcaa atcgccatcg gttggttgat
    37321 tgtggaagac ctggtaatgg ttctgacgct ggtgttgctg cccgcagtgg caggaatgat
    37381 ggaacagggc gatgtgggct ttgccactct tgcagtcgat atggggatca ccatcggcaa
    37441 agtgatcgcg tttatcgcca ttatgatgct ggtaggtcgc cgtctggtgc cgtggattat
    37501 ggcacgcagc gcggcaaccg gttctcgcga gctgtttacc ctgtcggtgc tggcgctggc
    37561 gttagggatt gcctttggtg cggtagagct gtttgatgtc tcctttgcac tcggtgcgtt
    37621 ctttgccggg atggtactga acgagtctga actgagtcac cgtgccgccc acgatacgct
    37681 gccattgcgc gacgcgtttg cggtgctgtt ttttgtctcc gtcgggatgt tgtttgatcc
    37741 gttaattctg attcagcaac cgctggcagt gctggcgacg ctggcgatta ttctgtttgg
    37801 taagtcgtta gccgcgtttt tcctggtgcg actgtttggt cactcccaac gtacggcatt
    37861 aactatcgcc gccagcctgg cgcagattgg tgagttcgcg tttatcctgg cgggactggg
    37921 aatggcattg aatttactgc cgcaggccgg acaaaacctg gtactggcag gggcgatcct
    37981 gtcgattatg ctcaacccag tactgttcgc actactggag aaatatctgg cgaagaccga
    38041 aacgctggaa gagcagacgc tggaagaggc aatcgaagaa gagaagcaga tcccagtgga
    38101 tatttgcaac catgcgctat tggtgggtta cggtcgtgta ggcagcctgc tgggggagaa
    38161 attgctcgcc tctgatattc cgctggtggt gattgagacg tcacgaaccc gtgttgatga
    38221 gctgcgagag cgcggggtcc gcgcagtatt gggcaatgcg gcgaacgaag aaattatgca
    38281 actggcgcat ctggaatgtg caaaatggct gatcctgacg atccccaacg gttatgaagc
    38341 gggtgagatt gtggcatctg cccgcgcgaa aaatccggat attgagatta ttgcccgcgc
    38401 ccattatgac gatgaagtgg cgtatatcac cgaacgtggt gcgaatcaag tagtgatggg
    38461 cgagcgtgaa attgcccgta ctatgctgga actgctggaa acgccaccgg cgggtgaagt
    38521 ggtgacgggg taaacggtgg ttgccggatg taaaatatga aaaatatgta ggcatgataa
    38581 gacgcgccag cgtcgcatca ggcattgaga gcaaaatgcc agatgcggcg tgaacgcctt
    38641 atctggccta cgaaccgacg ataacttaac gatcccagta agactcttcc aggctgtctt
    38701 cacgctccgg caagccgcgc gttaaacgag gtgaatgctg gttcagtacc tgatagctca
    38761 cacggttagc atatttacac acctgcgcta acgatgaata agttaaccag gtgaatttat
    38821 gtttgctgga gtttggtacg ttgctacgat ggtagctgtt ggcggtaatg tcatgcagca
    38881 acgctgccag tgcgccatcc cctgctccgt tggtgttcat gattttttcc ggcccgccca
    38941 tgtacggcgc aatgtgcgaa tagacacgca gcggattctg acaatccttg tggcgcatgg
    39001 cgcggctaaa ctcatactgg ttgaattcgg ctatagcgcc cggcagcaac ggatgctggg
    39061 ttttacgttt cgcttcgtct tcggtaaagc ccgccatata caagccgatt ggcccggcgg
    39121 tgcacagcac cagatctacc cagtccagcg ccttgtcaga tgccaacaac ggatcgcttt
    39181 ctccggtcaa cgcttcggct tcatcttcgt tcatcgcaag gatagagacg tggtctttga
    39241 ggaattgctg ccaccactgt ggattctcgg caatgacaaa cttagttccc agcgtcagca
    39301 ccaccggtac gttatatttc ttcgcgtact caatggcttt catggttgct tccggcatgg
    39361 gttcacccgg cttgcaacgc accagataag aggtgagaac cagagccgag gctccggcaa
    39421 tcacatcttc cggaatactt tcagcccgca gctggttcat gtggccaggg ctgatagcaa
    39481 aggtacgttc cccggactcg ccaatcagcg taaagcaacg accaatcggg ccatccacgc
    39541 cttgtagata gttaagatcg gtacggctgg aggtgttaca caggtaacga taggcatagc
    39601 tgccaatttc aatattgctg cacatgacgc ccagcagcac cgaacggtcg tccgcgagca
    39661 ccgagtagtt atgcatggtg ttaccaatgg tgccacccgc aaactgatgg gtaatcaggt
    39721 ttttctgttt tagttcctga taaagcgctt cggctacatc atcctcaatc accagtgaat
    39781 gcccggcgct taatccataa cgctcaatga attcatcatc cactttcgct tcaatatcga
    39841 ccagcgtttg atcgataccc actacccagg cggcgctggt ttcgttttct ggctggaatt
    39901 gctgaagcag cggatcgcgt gcatttacgg ggaagtaatg tttggattta cgtttaccgg
    39961 gaaatttcat ggttgttttt acgggtggtt aactgagcgg cgaatgttaa cacaatctga
    40021 cgggagcgcg cgagacgcag ccagtttcaa tatcagcagg cgggatcgca ttttcatggc
    40081 agtggttcct tacaatgacg taatttgaaa ggagtttttg tatgaagccg gaaaacaaat
    40141 tacctgttct ggaccttatt tctgctgaaa tgaagaccgt tgtgaatact cttcagccgg
    40201 atttaccgcc ctggcccgca acgggaacga ttgctgagca acgacagtat tacacgcttg
    40261 agcgccgatt ctggaatgcg ggcgctccag aaatggcaac caaagcttac atggttccaa
    40321 caaaatatgg gcaggtggaa acacgtctct tttgtccgca gccagatagc ccggcaacgc
    40381 tattttattt gcatggaggc ggttttattc tcggcaatct cgatacccac gatcgcatca
    40441 tgcgcctgct ggcaagctac agccaatgta cggtgattgg tattgattac accctttcac
    40501 ctgaagcgcg ttttccgcaa gtgatagagg aaattgtggc tgcttgttgt tatttccacc
    40561 agcaggcgga ggattatcaa atcaatatgt cccgcattgg ctttgccggt gattccgcag
    40621 gtgccatgct ggcgctcgcc agtgcgttgt ggttgcgtga taaacagatc gattgcggta
    40681 aagttgcggg cgttttgctg tggtatgggc tttacggatt acgggattcc gtgactcgtc
    40741 gtctgttggg cggtgtctgg gatggcttaa cgcaacagga tttgcagatg tatgaagagg
    40801 cgtatttaag caacgacgcg gaccgcgagt cgccgtatta ctgtctgttt aataatgatc
    40861 tcactcgcga agttccgccc tgttttattg ccggggcgga gttcgatccg ctgctggatg
    40921 acagccgtct gctttaccag acgttagcgg cgcatcagca gccctgtgag ttcaaactct
    40981 acccaggcac gctgcacgcc tttttgcatt attcacggat gatgaaaacc gccgacgagg
    41041 ctcttcgcga cggcgctcag ttctttaccg ctcagcttta gcgatacgcg gcaacaagat
    41101 tagccatcat ttcgatatgt tccggtgtgg cattaagcgc cggaatatat tcatattttt
    41161 tcccgccggc accgaggaag acctcacggt tttgctcggc aatctcttcc agcgtctcca
    41221 gacaatccgc agcaaagccc gggcacatca cctgaatatg acctacgcct ttttctccga
    41281 gcattttcag cgtttcgtcg gtataaggca tcagccaggg ttcccgacca aagcgcgact
    41341 gaaaggtcat catcactttt tccggtgcca tccctaatgc ggaagccagt tcacgagtcg
    41401 ttgtgcggca acgttgcggg taatcatcgc cttcatctgc ataacgctgg ggaatgccat
    41461 gataagagag cagcagcaga tccggttcgc catgtttggc aaaagaagcg cgtacgctgt
    41521 tcgccagtgc attaatgtaa tcgtggttat cggcgtaatc acgtataaac gatatccccg
    41581 gaatgctacg tttgcgcgcc agaatgcgtg ccagttcatc ccataccgca ccgaccgtag
    41641 aacaggagaa ttgcggataa agcggcagca ccacaatatg atctacatgc tctgccagga
    41701 gttcatctac ggcgctttcc agtgatggcg agccgtagct cattcccagc gctacgggcg
    41761 tctccggtaa acgttgtgcc agcgcctgct gttgctggcg gctgtaaacc atcagcggcg
    41821 agccaccttc catccagaca gaggcataca gcttcgccac acgcggcgag cgcagcggca
    41881 aaatcacgcc gcgcagcaat ggccaccata acaaccgtga ggtatcaacc acgcgtctgt
    41941 cgcttaaaaa ttgtttcaga tagcgtttta ccgcttcagg tgtgggggca tcgggcgtac
    42001 ccaggtttgc cagcaggata ccggttttag tctgacgcat taccgcctct tatcgattca
    42061 acttgttgat aattgtagcg gaaaagtgag gaagaagaac taattgctgt gagaggtatt
    42121 gcctgaggtg gatgagcaga cctgcccgaa agcagatctg ctgcggcttg attagccgag
    42181 gattttttcc agatcagcgc gaacttcagc aaccggcttg gtgccgtcaa ctttcgcgta
    42241 tttggtgtta cccgcttccg cttctttgga gtagtagccg atcagcggtg ctgtcatctg
    42301 atggtattca accagacgtt tacgtacggt ttcttcctga tcgtctttac gggtagtcag
    42361 ttcttcaccg gtaacgtcgt ctttaccttc tactttcggc ggattgaatt taacgtgata
    42421 aacacgacca gacggcgcgt gaacgcggcg accgacgata cggtcaacga tcagttcgtc
    42481 cggtacgtcg aattccagaa cgtaatcaac attgatgccc gcttctttca tcgcgtctgc
    42541 ctgcggaatg gtacgcggga agccgtccaa caggaaacca ttacggcagt cttcctgagc
    42601 aatgcgctct ttaaccagcg cgatcaccag ttcgtcggtg accagtttgc cagcatccat
    42661 aatgtctttt gcttgtttac ccagctcgga gccagatttg accgcagcac gcagcatatc
    42721 gccagtggag atttgcggaa taccatattt ctccatgatg aactgagcct gagtcccttt
    42781 ccccgcaccc ggagcgccaa gcagaatgat acgcattgcg aaaatcccct taaaatgtgt
    42841 cgaatttttt gaaaaagcga taaacgatac caccatcagg cgattgcctc aagaaaggca
    42901 ccaggggctg aaacggttaa tggcgagata atttgcggaa agtgcagata atgaaaagaa
    42961 aaatgccgga tgacacgaag gtcatccggc attacatcag gaaaccagca gctggttcat
    43021 acgacgaata aacaggttcg gatcttccag cgtgccgcgt tctgccagca gtgcctgatc
    43081 cagcagcagt tctacccact cgctgaactt cgcttcatct tcagtatctg ccgcacgttt
    43141 caccagtacg tgatccgggt tcagttcgaa gatgtatttc acttctggca ctttctggcc
    43201 cgccgcagcg aacagtttcg ccatctgagt gctcatttcg tccgcgtcgg tcgaaacgat
    43261 cgctggcgta tcggtcagac ggtgagtcag acggacatct ttcacgcgct cgccgagcag
    43321 ggctttcaca cggtcgatga acggagtcag tgctttctcc gcttctttcg cgctctcatc
    43381 aacttcgtca gccagttttt ccagcgactc gtcaacttta gacaccgact ggaacggttt
    43441 accgtcgaac tcagtcagat agttcatcat ccactcatcg atgcggtcgg aaagcagcag
    43501 aacttcgatg cctttcttac gcagcagttc caggtgcggg ctgctcttcg ctgccgcata
    43561 gctgtctgca gtgatgtagt agattttctc ctgcccttct ttcatgcggg aaacgtagtc
    43621 ttccagagac acggtctgcg cagaagaatc ggtatgggta gaagcaaaac gcagcaattt
    43681 ggcgatcgct tcctggttag cgaaatcttc cgccggacct tctttcagta ccaggccaaa
    43741 ctgttgccag aaggtctggt atttttccgc gtcgtctttc gccagttttt ccagcatttg
    43801 cagcacacgc ttggtcagcg cattgcgcag gttacgcgtt accgtgctgt cctggaggat
    43861 ttcacgggaa acgttcagcg gcagatcgct ggagtcaatc agaccacgca cgaagcgcag
    43921 atagttcggc atgaactgtt ctgcgtcgtc catgatgaac acacgctgaa catacagttt
    43981 caggccgtgt ttatgatcgc ggttccacat atcccacgga gcctgggacg gaatgtacag
    44041 caggctggtg tactcctgct taccttcaac acggttgtgg ctccaggtca gcggatcgtt
    44101 aaaatcgtgg gcgatgtgtt tgtagaactc tttgtactct tcatcggtga tttccgactt
    44161 gttacgagtc cacagcgcct gcgctttgtt aattttctcc caggagataa cggtttcgcc
    44221 gtctttctct tcgcgttttt cgatctctac cggcagcgcg atatggtcgg agtatttgct
    44281 gatgatggaa cgcacgcgcc agtcatcgag gaactcgtct tcgccttcac gcagatgcag
    44341 ggtgatttca gtaccacgat cttctttggt gatgtcggca acggtatatt caccttcgcc
    44401 agccgattcc cagaagacgc cgttttctgg tttttcgcct gccgcgcgag tacgcacggt
    44461 cactttgtcg gccacgataa acgcagagta gaaaccaaca ccaaactgac cgatcagctg
    44521 gctgtctttc gcctggtcag aacccaggga ttcgaggaat gatttggtac cggatttagc
    44581 gatagtcccc agatggtcaa tcacttcgtc gcgggtcatc cccacgccgt tatcggagat
    44641 ggtcagcgtg cgcttgtctt tatcgaaaga gacacgaacg cgcagttcgc catcaccttc
    44701 gtacaggtcc gggttagaga gcgcacggaa acgcaacttg tccgccgcat cggaggcgtt
    44761 agagataagc tcacgcagga agatttcttt attggaatag agagaatgga tcatcaggtg
    44821 cagaagctgt ttcacttctg actgaaaacc acgagtttct tgtcctttca tgtaggtcta
    44881 cctcaataat gccattttaa ggttaaaaac aggatgaggg agagatgggg acaagggaga
    44941 ataatttcaa gcgagagcag gtgatcctgc tctcgtttgc ttaaaaacga atcttatgac
    45001 gcccggcaag ggagtgtgac agcgtggtgc catcgaccat ttccagttcg ccgccaaccg
    45061 gtacgccatg agcgattcgg ctggcttcca cgtcatattg cgcgcaaagc tcggcaatgt
    45121 agttagcggt agcttcacct tcaaccgtag ggttggtggc gaggatcact tcagtgattt
    45181 tttcctctgc cagacgctgt tccagacgat caaggccgat atcatccgga ccgatgccgt
    45241 ccagcggtga cagatgtccc atcaacacaa aataacgacc tgaaaactgc cccgtctgct
    45301 caatggcgta aatgtccgcc ggactctcca ccacgcagat ttgaccgttt tcctgacgac
    45361 gcggattcga acagatgtta cagacttcct gttcggtgaa ggtgcggcaa tcggcgcagt
    45421 ggccgatttc cgacatcgcc cgggtgagcg cctgcgccag acgcatcccg ccgctacgat
    45481 cgcgctgaag cagcgtgaac gccatacgct gcgccgactt cgggccaacg cccggcagac
    45541 agcgcagtgc ttccataagc tgtgttaaca gcgggctggt ttgcatcaga acggcatctt
    45601 aaagccaggc ggcagctgca ttccggagga tacagaagcc attttttctt tctgcgtttc
    45661 ttcaatacga cgtgctgcgt cgttgaatgc tgcagccacc aggtcttcca gcatctcttt
    45721 gtcgtcttcc agcaggctcg ggtcgatctc tacgcgacgg cagttatgtg caccgttgat
    45781 ggtcactttt accagacctg cgccagattc gccggtaact tccagctgcg cgatctcttc
    45841 ctgcattttc tgcatttttt cttgcatctg ctgggcttgc ttcatcaggt tacccagacc
    45901 gcctttacca aacataggtt tctctctcaa tcacgttaag gatgacgaac gtaagctgtg
    45961 cttacgatca aatggggcgg atactgtctt catccagctc cgcatcgaag aaccgacgca
    46021 gggtctgaat attattatcc gcaataatgg actcgcgcgc ctgcgcaagt ttttcttcgt
    46081 atatcgcctg acgccactcc agcggcgtac gcaccgcggg attatcatct tcaactatag
    46141 tcagttcaac cgttgaacct tttaacatgc tcaacgcttc agccagtttt tgctgtgcac
    46201 cgcggttgtt caaatgccgc tgagaggagc gcaaatgcag acatactgcg ttgtcgctct
    46261 cctctttcca ggcatttaac gccacctgtt cgaccagttt tggtagcgaa agttgactca
    46321 cctgagccgc ccacgcgtcg cgctcaatgg cttctgccgc cagcttcgcc gccagttccg
    46381 gcgttttttc atgttccagc gcttttttca gcgccttcgg tgtggcgacc acttcttttt
    46441 gctgcatcac tggagtggtc gccttccagc gatacgcttc ttttttagct ggcgcttttt
    46501 ccagcgccga tggcaccgga cgcgcctgaa cgcgatcggt gaccgaagcc agtctttcca
    46561 gcgcagcgtt attcaccggc cgcgcgcggg tagcggctgc cggttcactc ttttttgctt
    46621 tggttgctcc ctgcacgcgc tgcaactgct ggcgcgccgc cagcacctgg ctggtggttt
    46681 ccgggagcgg tacagtcggt gcctgctgcg gcgctgattg cggttgcggc ggcacctggg
    46741 ttggcgtcat tactgccgtt ggcgcgacgg gtgcaaagga ctggcgtggc acttctggct
    46801 caggcagcgg catacgcgga tggaacgcca gcgcgcgcag cagcgtcatc tcaacgccca
    46861 tgcggcggtc cggcgcatac ggtaattctt tgcgaccaat caacagcgtc tgatagtaaa
    46921 gctgaatatc cgtcggcggt atggtgcgcg ccagttcacg catccgcagc tcgatggcgg
    46981 ccatgtcgtt gccaagtgca gcaggcgaaa gttgtaccat cgcaatacga tgcaacaggc
    47041 cgagcatttc caccagcaac gcttcccact cgataccacg ggcagcggct tcattaatca
    47101 gcgccattac gcgctcgccg ttggcctcga ccatcgcttc aaccagcgac agcgcctgat
    47161 cgtcgtcaag cgtacccagc atcgcactga ccgcctgggt tgaaacctgg ccgtcaccgc
    47221 tggcaatcgc ctggtcggtc agacttaagg catctcgcag gctgccttca gcggcgcgcg
    47281 ccagcaattg cagcgcccgc ggctcgtgag cgatatgttc ttcattgagg atgtgctcaa
    47341 gttgatgacg aatttgctcg acatccagcg ccttgagatg aaattgcaaa cagcgcgaca
    47401 aaatcgtcac cggcaatttc tgtggatcgg tcgtcgccag cagaaactta acgtgctccg
    47461 gcggctcttc aagggttttt aacagtgcgt taaagctgtg gcgcgacagc atatgcactt
    47521 cgtcgatgag ataaactttg aaacgaccac gcgccggagc gtactggacg ttatccagca
    47581 ggtcgcgggt atcttcaact ttggtgcgcg aggcggcgtc gatttcaatc agatcgacaa
    47641 agcgcccctg ctcgatttca cgacagttat cgcacacgcc gcacggcgtc gcggtaatgc
    47701 cggtttcgca gtttagcccc ttcgccagca gtcgggcgat agaggttttt ccgacgccac
    47761 gggtgccgga aaaaagataa gcatgatgaa tacgccctaa cgacaagccg ttcgccagtg
    47821 cggtcagcac atgttcctgg ccgacgacgt cagcaaaggt ttgtgggcgc cattttcggg
    47881 ctaagacctg ataactcatt ggcaggctct gaaacgctgg aaggtggatt ctcgaagggg
    47941 taatgctaac acagccccgt cagaacggcg aggctgaatg cacatcttgt gctgtccgta
    48001 gcgtgggcag cacaagactg gcgataatta atggcccggg aacgggacaa ggctgtagct
    48061 ggtaatgccc tgtttttcga gacgctgttc gccgccgaga tcgaacaggt tgataatgaa
    48121 cgcagcgtca gccacttcac cacccagacg acggatcagt ttaacggtcg cttcgatagt
    48181 gccgccggtt gccagcagat cgtccaccac cagaactttg tcgcccggtt tgatggcatc
    48241 aacgtggatc tccagctgat cggtgccgta ttccagatcg taagtttcac tgatggtttc
    48301 acgcggcagt ttgcccggtt tacggaccgg tacaaagcca acgcccagac ccagagctac
    48361 cggagcgcca aacaagaagc cacgcgcttc ggtgccgaca actttgttaa tgcccgcatt
    48421 tttgtaacgc tcaaccagca agtcgatgct gagagcgtaa gctttcgggt cttccagtaa
    48481 gctggtgaca tcgcggaaaa gaatgccggg ttttgggtag tcctgaatgc ttttgatgct
    48541 atttttgaga tactcaagct gctgtgcagt cgcggtcata agtgtatgcc tgcttgttac
    48601 ggtggtactc acggcgcgtt ttaaacgtat caaaagtaac agttgtttaa cctttgaccg
    48661 cctgcgcctg tgctcgaaaa cgccagaatg tactggctgt acgcaataat tgcaactgcg
    48721 attgttgtgc ttcagtgctt ttcttgcttt tcatcaatca ccggaattcg ccacatatag
    48781 aaaagcaaac aggcgagaat taccagcaac atgatgcgca cccatggcat ctggacgaac
    48841 cacagagaaa tggcaaacgt gagcaaaata agcaaaatcg cccgcggttt gacgccgcgc
    48901 ggcatcgcat gatgtttctg ccagaaacgt agatagctgc caaaccatga gcggtacagc
    48961 aaccaggcgt gaaagcgcgg ggaagaacgg gcaaagcacc aggccgccag caggataaac
    49021 ggcgtcgtcg gtaataccgg taataccacg cccagcgtgc ccagcactac cgccagccag
    49081 ccaatgatga ttaaaatgat tcgttgcata ttgagtgttg tcagcttaca gagtggctac
    49141 tttagcataa caattatcat tttcattgag gtcttatcgt gaaaaccgcc ctgctgctgg
    49201 aaaaactgga aggacagctc gctacgctgc gtcagcgttg tgccccggtg tcacagttcg
    49261 ccacgctaag tgctcgtttc aacaggcatc tttttcagac tcgtgcgaca acactacagg
    49321 cttgtctcga caaggcgggc gataatctgg ctgcgcttcg tcatgcagtt gagcaacagc
    49381 aactgccgca agtcgcctgg ctggcagaac atctggcggc gcaactggag gctatagcgc
    49441 gtgaagccac agcctggtcg ctacgcgagt gggatagcgc accaccccaa attgcccgct
    49501 ggcagcgtaa acgtattcag catcaggatt ttgagcggcg gctacgtgag atggttgccg
    49561 aacgtagagc ccgcctggcg cgggtgaccg atctcgtgga acagcaaacg ctgcatcgtg
    49621 aagtggaagc ctatgaagcg cgcctggcac gctgccgcca tgcgctggaa aaaatcgaaa
    49681 acaggttagc gcgtttaacc cgctagcaat ggagagagta tgtcactgga aaatgcccct
    49741 gacgatgtca aactggctgt cgatttgatt gtgctgctgg aagaaaatca gatcccagcc
    49801 cgcaccgtgc tgcgcgcgct ggatattgta aagcgtgatt atgaaaagaa gttacagagc
    49861 gatgaggctt cacaaagcga gtaaaaaata aagggcggca tcactgtcgc cctttattgt
    49921 ccttacccta ccgctggcgt cggatcgtcg cctttgtagt cgcgttttac ttccgtcacc
    49981 tcatcgccct tctcgttgtg cagatgcact tcaagctggt taaaggcaat gttgatgtcg
    50041 ttttcacggc acagctgatc gatagtacgg ttcagctcat cgacagtacg actacggtca
    50101 cgcagttcac gcacatacag acgcagctca tgatccaacg tgctggcacc aaatgccgta
    50161 aagaagactt ccggcattgg ttcgtgcatc acccttgggt gctcagtcgc cgccttcagt
    50221 aacactttac gcactttttc cagatcggag ccataggcca cgccgagacg gatcaccaga
    50281 cgcgtagtag tgtcagtcaa cgaccagttg atcagacgct cggtaacaaa cgctttgttc
    50341 gggatgatca cttctttgcg atcgaaatcg gtaatcgttg tcgcacgaat acggatttta
    50401 cttaccgtcc ccgagaagct accaatggtt accgtatcgc caatacgcac cggacgttcg
    50461 aacagaatga tcaaaccgga gacgaagtta ccgaaaattt cttgtaaacc aaaaccaaga
    50521 cctacggata atgctgcggc cagccactgg agtttatccc aagagacgcc cagcgatccg
    50581 aacaccgtca tcgcaccaac agcaataatg atgtagttaa ggatggtagt aatggcatac
    50641 gacgcgccct ggcgcatatt cagtcgcgag agcaccagca cttccagcaa accaggcagg
    50701 ttgcgaatca acgcccaggc caccattgag gcgataatcg caaacaacag actacccatg
    50761 gtgacgtttt tcaccaccgc agcaccagct tcagtaccgt tgtaatgcca cagcgtaatg
    50821 ctgtcgagat agctgaacac ggtgatcaaa tcggaccaaa ttgcccagaa catgacaccg
    50881 aacagcgcaa acatcagcaa catggtaata cgcagcgtct gctggttaac ctgttccagt
    50941 gcaatggtgg gttcttccgg cggttcggca ccttctgcgc cctctttcac cagattctgc
    51001 cgacgcgcca gcgcacgacg ccaggcgata cgccgcgccg ctacgcttaa gccgcgcaat
    51061 accgtctggt agagcaagtt ccagatgatc accagataaa cggtttcaat ccaacgcccg
    51121 gagaggcgca gcgtggtgta gaaataaccc gtggcagtca gcaccatcaa tgcaatcggg
    51181 ataatcgaca gcacggtaat ggtgaccagt cgcatggtgt gcgactcttt atcacgccag
    51241 ctttcgcggc acattggcca taccaggaag gcaatcagca gcaggttgaa gaaaatcatc
    51301 gcctgcccca gcacatcatc catcagatgc agcggggaaa gttctgccac cacagaccag
    51361 aaatggatag gcagcaacgc gagactgatg cggacaattt gccgacgcca gtggctggtc
    51421 tgctgttccg gcatgccgaa gtgacgcacg gcaacgccgt tcttctccag cactttccag
    51481 cacaggccaa acaccagcca gaaaatcgcc agttttttgc tgaacgacca tagcagttcg
    51541 ctgatgttga gctgcatggt caacagaatc aggccaaccg cgagaataat caggcacacc
    51601 ggcagtgcac ggatcaggtc gataaggatc gcttttggtg tgttgagctg gctgtcgtta
    51661 cgcagggaac ccacagcgga agccagtttt tgttgatacg ctttcagcca ccccagacgc
    51721 cagtggatca gcccggcaat caacagcagc ggcaaaccag cgaggaaagc gataaaaacg
    51781 gcgggccagg ctttttccca gttaaccgta attttcatcg acttaaattc atctttcagg
    51841 ctttgcggga acgctttgat ccagtcccag tccattggac ggttactgtt cacccaaaag
    51901 atttgctgag tcaggatgga tttcaggttt ttcgacacac tcattaactg ctgctggttg
    51961 atttgcaggt taatggccat catcagctgg ttacccaact gtttgttgag ttgatccagc
    52021 aattcgcgac gcatatcaac cacttgcaat aacgcatcgt gaacttcgct gttgacttcg
    52081 ttggtgtgac cttcttccag tttgttgacg aacgcatcgc tctggaagag tgcatcacgc
    52141 tgctggttaa cttcaaactg ttcgagacgc aaatccgcga tgcggttggt catgttttcc
    52201 agttcatccg ccgagggcag cgtttgttgt tgctggtaaa ggatacgaga caacagcagg
    52261 ctgcccttca ggacggcaat ctgctcttta atattgcgtt ccgattgcag cgcccgctcc
    52321 agccagtttt tgactttaat gttttgctgc atcaactgat taccgttttc agtcgcggta
    52381 atcagacgct gacttaactg ctggttaatt tccagttcct gcttcaccag cggattagcc
    52441 tgaatacgcg cggcttcatc cggggagacg gcttcctgcg ccgttttttc ggttaaagtc
    52501 aggcgcttgc tgtttaccgc ttcttgcaac agctgcaact ggtgctccag acgggcgctg
    52561 ttcgccgtca cgtaatcacg ttgcttttgc aaggtatcct gcaagacggt attcccttcc
    52621 aggcttttac gctgctggtc aatctccgca ttcagcaacg cctgctgagc ctgcattaac
    52681 actttctggc tgggacgtaa ggccgtctcg ccgacatcag tcccatccag acgactgcga
    52741 atttgttgca gctgctgcga agcgttatac atcgcatttt gcacgcgttc gggctgcgtc
    52801 tgtaacgaaa ccagctggct gttataagac gccagatcgt tttgtgcgtt ttgcaaatcg
    52861 tccagcgcct gggcaacgcg agtttccagc tggcgcaacg aaagcgtgct cagaattttg
    52921 cgcgtttctt cgtcgttatc gacatcgcta agtgctgtta acgccgcggt cgcctggcgc
    52981 attttttccg gcgcttcagc gactttttgc cgtagctgaa cggtctcttc ttttacgcga
    53041 tcgattttat cgagggtggc taatgtatct gtcagatcct gctgcaccag tttgtcctga
    53101 gcagaaagat ctttttgttt atttagtgag tcaagttgcg cctgcaggtc cgcttttgtc
    53161 ggcagatcac cattcgatga cgcccgcgca aacgccgtgt tctggcataa caagacaaaa
    53221 acaaaagcaa taaatgctga aaaaacaaaa tgccgtgatc gtttgtaata ctggaacata
    53281 gtcatgatga atgaaggttt ctgaacctga agaacgacct gaaaaagtca aaccgcaaga
    53341 atatcacgac gcagtgaacc agaatagcaa cgacgaaaat gtccaggaaa aatcctggag
    53401 tcagattcag ggttattcgt tagtggcagg attacgaagc gtggggcaca ggagatacat
    53461 ctccagtaag atggcgacgt aatcgcgggc ttctttttta agatcaaaag attgcggggc
    53521 aaagagccag ttttccatca ggccggaaat atagccgcgc ataataattg ctgcccgacg
    53581 cgtcattaaa tccgcaggca acattttcgc ttcaatacaa tgttttaacg tttgttctat
    53641 acggtcataa ctttccaaac agagattacg ttgtgcctgt tgcacaacag ccatttctcc
    53701 gacaaattcg catttgtgga atataatctc catcaataat cgacgccgtt cttctgtcac
    53761 cgtggattca agaacatgaa ttaatatctc tcttaatact gagagtggat cgccagggaa
    53821 ttttgcctga tactcaagct ctagttcacc aatattggat tctgatagtt cccagatctc
    53881 actgaacaaa tccgacttgt ctttaaaatg ccagtagatt gcaccgcgcg taacgccagc
    53941 tgcttttgca atctcaccca gcgaggtgga tgataccccc tgctgtgaga aaagacgtag
    54001 agccacatcg aggatgtgtt ggcgcgtttc ttgcgcttct tgtttggttt ttcgtgccat
    54061 atgttcgtga atttacaggc gttagattta catacatttg tgaatgtatg taccatagca
    54121 cgacgataat ataaacgcag caatgggttt attaactttt gaccattgac caatttgaaa
    54181 tcggacactc gaggtttaca tatgaacaaa aacagagggt ttacgcctct ggcggtcgtt
    54241 ctgatgctct caggcagctt agccctaaca ggatgtgacg acaaacaggc ccaacaaggt
    54301 ggccagcaga tgcccgccgt tggcgtagta acagtcaaaa ctgaacctct gcagatcaca
    54361 accgagcttc cgggtcgcac cagtgcctac cggatcgcag aagttcgtcc tcaagttagc
    54421 gggattatcc tgaagcgtaa tttcaaagaa ggtagcgaca tcgaagcagg tgtctctctc
    54481 tatcagattg atcctgcgac ctatcaggcg acatacgaca gtgcgaaagg tgatctggcg
    54541 aaagcccagg ctgcagccaa tatcgcgcaa ttgacggtga atcgttatca gaaactgctc
    54601 ggtactcagt acatcagtaa gcaagagtac gatcaggctc tggctgatgc gcaacaggcg
    54661 aatgctgcgg taactgcggc gaaagctgcc gttgaaactg cgcggatcaa tctggcttac
    54721 accaaagtca cctctccgat tagcggtcgc attggtaagt cgaacgtgac ggaaggcgca
    54781 ttggtacaga acggtcaggc gactgcgctg gcaaccgtgc agcaacttga tccgatctac
    54841 gttgatgtga cccagtccag caacgacttc ctgcgcctga aacaggaact ggcgaatggc
    54901 acgctgaaac aagagaacgg caaagccaaa gtgtcgctga tcaccagtga cggcattaag
    54961 ttcccgcagg acggtacgct ggaattctct gacgttaccg ttgatcagac cactgggtct
    55021 atcaccctac gcgctatctt cccgaacccg gatcacactc tgctgccggg tatgttcgtg
    55081 cgcgcacgtc tggaagaagg gcttaatcca aacgctattt tagtcccgca acagggcgta
    55141 acccgtacgc cgcgtggcga tgccaccgta ctggtggttg gcgcggatga caaagtggaa
    55201 acccgtccga tcgttgcaag ccaggctatt ggcgataagt ggctggtgac agaaggtctg
    55261 aaagcaggcg atcgcgtagt aataagtggg ctgcagaaag tgcgtcctgg tgtccaggta
    55321 aaagcacaag aagttaccgc tgataataac cagcaagccg caagcggtgc tcagcctgaa
    55381 cagtccaagt cttaacttaa acaggagccg ttaagacatg cctaatttct ttatcgatcg
    55441 cccgattttt gcgtgggtga tcgccattat catcatgttg gcaggggggc tggcgatcct
    55501 caaactgccg gtggcgcaat atcctacgat tgcaccgccg gcagtaacga tctccgcctc
    55561 ctaccccggc gctgatgcga aaacagtgca ggacacggtg acacaggtta tcgaacagaa
    55621 tatgaacggt atcgataacc tgatgtacat gtcctctaac agtgactcca cgggtaccgt
    55681 acagatcacc ctgacctttg agtctggtac tgatgcggat atcgcgcagg ttcaggtgca
    55741 gaacaaactg cagctggcga tgccgttgct gccgcaagaa gttcagcagc aaggggtgag
    55801 cattgagaaa tcatccagca gcttcctgat ggttgtcggc gttatcaaca ccgatggcac
    55861 catgacgcag gaggatatct ccgactacgt ggcggcgaat atgaaagatg ccatcagccg
    55921 tacgtctggc gtgggtgacg ttcagttgtt cggttcacag tacgcgatgc gtatctggat
    55981 gaacccgaat gaactgaaca aattccagct aacgccggtt gatgttatta ccgccatcaa
    56041 agcgcagaac gcccaggttg cggcgggcca gctcggtggt acaccgccgg tgaaaggcca
    56101 acagcttaac gcctctatta ttgctcagac gcgtctgacc tctactgaag agttcggcaa
    56161 aatcctgctg aaagtgaatc aggatggttc ccgcgtactg ctgcgtgatg tggcgaagat
    56221 tgagctgggt ggtgagaact acgacatcat cgcagagttt aacggccaac cggcttccgg
    56281 tctggggatc aagctggcga ccggtgcaaa cgcgctggat accgctgcgg caatccgtgc
    56341 tgaactggcg aagatggaac cgttcttccc gtcgggtctg aaaattgttt acccgtacga
    56401 caccacgccg ttcgtgaaaa tctctattca cgaagtggtt aaaacgctgg tcgaagcgat
    56461 catcctcgtg ttcctggtta tgtatctgtt cctgcagaac ttccgcgcga cgttgattcc
    56521 gaccattgcc gtaccggtgg tattgctcgg gacctttgcc gtccttgccg cctttggctt
    56581 ctcgataaac acgctaacaa tgttcgggat ggtgctcgcc atcggcctgt tggtggatga
    56641 cgctatcgtt gtggtagaaa acgttgagcg tgtgatggcg gaagaaggtt tgccgccaaa
    56701 agaagccacc cgtaagtcga tggggcagat tcagggcgct ctggtcggta tcgcgatggt
    56761 actgtcggcg gtattcgtac cgatggcctt ctttggcggt tctactgggg caatttatcg
    56821 tcagttctct attaccattg tttcagcaat ggcgctgtcg gtactggtgg cgttgatcct
    56881 gactccagct ctttgtgcca ccatgctgaa accgattgcc aaaggcgatc acggggaagg
    56941 taaaaaaggc ttcttcggct ggtttaaccg catgttcgag aagagcacgc accactacac
    57001 cgacagcgta ggcggtattc tgcgcagtac ggggcgttat ctggtgctgt atctgatcat
    57061 cgtggtcggc atggcctatc tgtttgtgcg tctgccaagc tccttcttgc cagatgagga
    57121 ccagggcgta tttatgacca tggttcagct gccagcaggt gcaacgcagg aacgtacgca
    57181 gaaagtgctc aatgaggtaa cgcattacta tctgaccaaa gaaaagaaca acgttgagtc
    57241 ggtgttcgcc gttaacggct tcggctttgc gggacgtggt cagaataccg gtattgcgtt
    57301 cgtttccttg aaggactggg ccgatcgtcc gggtgaagaa aacaaagttg aagcgattac
    57361 catgcgtgca acacgcgctt tctcgcaaat caaagatgcg atggttttcg cctttaacct
    57421 gcccgcaatc gtggaactgg gtaccgcaac cggctttgac tttgagctga ttgaccaggc
    57481 tggccttggt cacgaaaaac tgactcaggc gcgtaaccag ttgcttgcag aagcagcgaa
    57541 gcaccctgat atgttgacca gcgtacgtcc aaacggtctg gaagataccc cgcagtttaa
    57601 gattgatatc gaccaggaaa aagcgcaggc gctgggtgtt tctatcaacg acattaacac
    57661 cactctgggc gctgcatggg gcggcagcta tgtgaacgac tttatcgacc gcggtcgtgt
    57721 gaagaaagtt tacgtcatgt cagaagcgaa ataccgtatg ctgccggatg atatcggcga
    57781 ctggtatgtt cgtgctgctg atggtcagat ggtgccattc tcggcgttct cctcttctcg
    57841 ttgggagtac ggttcgccgc gtctggaacg ttacaacggc ctgccatcca tggaaatctt
    57901 aggccaggcg gcaccgggta aaagtaccgg tgaagcaatg gagctgatgg aacaactggc
    57961 gagcaaactg cctaccggtg ttggctatga ctggacgggg atgtcctatc aggaacgtct
    58021 ctccggcaac caggcacctt cactgtacgc gatttcgttg attgtcgtgt tcctgtgtct
    58081 ggcggcgctg tacgagagct ggtcgattcc gttctccgtt atgctggtcg ttccgctggg
    58141 ggttatcggt gcgttgctgg ctgccacctt ccgtggcctg accaatgacg tttacttcca
    58201 ggtaggcctg ctcacaacca ttgggttgtc ggcgaagaac gcgatactta tcgtcgaatt
    58261 cgccaaagac ttgatggata aagaaggtaa aggtctgatt gaagcgacgc ttgatgcggt
    58321 gcggatgcgt ttacgtccga tcctgatgac ctcgctggcg tttatcctcg gcgttatgcc
    58381 gctggttatc agtactggtg ctggttccgg cgcgcagaac gcagtaggta ccggtgtaat
    58441 gggcgggatg gtgaccgcaa cggtactggc aatcttcttc gttccggtat tctttgtggt
    58501 ggttcgccgc cgctttagcc gcaagaatga agatatcgag cacaaccata ctgtcgatca
    58561 tcattgatac aacgtgtaat cactaaggcc gcgtaagcgg ccttttttat gcataaccta
    58621 cgaacattaa ggagtaattg aaccaccaac tcaggatctc atacgaaaa
//
LOCUS       DAZFBR010000015        57946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFBR010000015 DAZFBR010000000
VERSION     DAZFBR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.015"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            123..2657
                     /gene="mrcB"
                     /locus_tag="ACHJZ2_001099"
     CDS             123..2657
                     /gene="mrcB"
                     /locus_tag="ACHJZ2_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO4567257.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2877..5120
                     /gene="fhuA"
                     /locus_tag="ACHJZ2_001100"
     CDS             2877..5120
                     /gene="fhuA"
                     /locus_tag="ACHJZ2_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO4567258.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5171..5968
                     /gene="fhuC"
                     /locus_tag="ACHJZ2_001101"
     CDS             5171..5968
                     /gene="fhuC"
                     /locus_tag="ACHJZ2_001101"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO4567259.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5968..6858
                     /gene="fhuD"
                     /locus_tag="ACHJZ2_001102"
     CDS             5968..6858
                     /gene="fhuD"
                     /locus_tag="ACHJZ2_001102"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO4567260.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6855..8837
                     /gene="fhuB"
                     /locus_tag="ACHJZ2_001103"
     CDS             6855..8837
                     /gene="fhuB"
                     /locus_tag="ACHJZ2_001103"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO4567261.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8872..10152)
                     /gene="hemL"
                     /locus_tag="ACHJZ2_001104"
     CDS             complement(8872..10152)
                     /gene="hemL"
                     /locus_tag="ACHJZ2_001104"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO4567262.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10318..10365
                     /gene="yadX"
                     /locus_tag="ACHJZ2_001105"
     CDS             10318..10365
                     /gene="yadX"
                     /locus_tag="ACHJZ2_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO4567263.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10377..11798
                     /gene="clcA"
                     /locus_tag="ACHJZ2_001106"
     CDS             10377..11798
                     /gene="clcA"
                     /locus_tag="ACHJZ2_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO4567264.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MALDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11822..11887
                     /gene="yadW"
                     /locus_tag="ACHJZ2_001107"
     CDS             11822..11887
                     /gene="yadW"
                     /locus_tag="ACHJZ2_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO4567265.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11880..12224
                     /gene="erpA"
                     /locus_tag="ACHJZ2_001108"
     CDS             11880..12224
                     /gene="erpA"
                     /locus_tag="ACHJZ2_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO4567266.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12271..12894)
                     /gene="yadS"
                     /locus_tag="ACHJZ2_001109"
     CDS             complement(12271..12894)
                     /gene="yadS"
                     /locus_tag="ACHJZ2_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO4567267.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRHLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12932..13732)
                     /gene="btuF"
                     /locus_tag="ACHJZ2_001110"
     CDS             complement(12932..13732)
                     /gene="btuF"
                     /locus_tag="ACHJZ2_001110"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO4567268.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13725..14423)
                     /gene="mtnN"
                     /locus_tag="ACHJZ2_001111"
     CDS             complement(13725..14423)
                     /gene="mtnN"
                     /locus_tag="ACHJZ2_001111"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO4567269.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14507..16024
                     /gene="dgt"
                     /locus_tag="ACHJZ2_001112"
     CDS             14507..16024
                     /gene="dgt"
                     /locus_tag="ACHJZ2_001112"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO4567270.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16154..17578
                     /gene="degP"
                     /locus_tag="ACHJZ2_001113"
     CDS             16154..17578
                     /gene="degP"
                     /locus_tag="ACHJZ2_001113"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO4567271.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17733..18890
                     /gene="cdaR"
                     /locus_tag="ACHJZ2_001114"
     CDS             17733..18890
                     /gene="cdaR"
                     /locus_tag="ACHJZ2_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO4567272.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(18979..19365)
                     /gene="yaeH"
                     /locus_tag="ACHJZ2_001115"
     CDS             complement(18979..19365)
                     /gene="yaeH"
                     /locus_tag="ACHJZ2_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO4567273.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19680..20504)
                     /gene="dapD"
                     /locus_tag="ACHJZ2_001116"
     CDS             complement(19680..20504)
                     /gene="dapD"
                     /locus_tag="ACHJZ2_001116"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO4567274.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20535..23207)
                     /gene="glnD"
                     /locus_tag="ACHJZ2_001117"
     CDS             complement(20535..23207)
                     /gene="glnD"
                     /locus_tag="ACHJZ2_001117"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO4567275.1"
                     /translation="MNPLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23269..24063)
                     /gene="map"
                     /locus_tag="ACHJZ2_001118"
     CDS             complement(23269..24063)
                     /gene="map"
                     /locus_tag="ACHJZ2_001118"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO4567276.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24431..25156
                     /gene="rpsB"
                     /locus_tag="ACHJZ2_001119"
     CDS             24431..25156
                     /gene="rpsB"
                     /locus_tag="ACHJZ2_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO4567277.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25414..26265
                     /gene="tsf"
                     /locus_tag="ACHJZ2_001120"
     CDS             25414..26265
                     /gene="tsf"
                     /locus_tag="ACHJZ2_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO4567278.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26412..27137
                     /gene="pyrH"
                     /locus_tag="ACHJZ2_001121"
     CDS             26412..27137
                     /gene="pyrH"
                     /locus_tag="ACHJZ2_001121"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO4567279.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27287..27844
                     /gene="frr"
                     /locus_tag="ACHJZ2_001122"
     CDS             27287..27844
                     /gene="frr"
                     /locus_tag="ACHJZ2_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO4567280.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            27936..29132
                     /gene="ispC"
                     /locus_tag="ACHJZ2_001123"
     CDS             27936..29132
                     /gene="ispC"
                     /locus_tag="ACHJZ2_001123"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO4567281.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            29321..30079
                     /gene="ispU"
                     /locus_tag="ACHJZ2_001124"
                     /gene_synonym="uppS"
     CDS             29321..30079
                     /gene="ispU"
                     /locus_tag="ACHJZ2_001124"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO4567282.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30092..30949
                     /gene="cdsA"
                     /locus_tag="ACHJZ2_001125"
     CDS             30092..30949
                     /gene="cdsA"
                     /locus_tag="ACHJZ2_001125"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4567283.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            30961..32313
                     /gene="rseP"
                     /locus_tag="ACHJZ2_001126"
     CDS             30961..32313
                     /gene="rseP"
                     /locus_tag="ACHJZ2_001126"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO4567284.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32343..34775
                     /gene="bamA"
                     /locus_tag="ACHJZ2_001127"
     CDS             32343..34775
                     /gene="bamA"
                     /locus_tag="ACHJZ2_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO4567285.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            34896..35381
                     /gene="skp"
                     /locus_tag="ACHJZ2_001128"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             34896..35381
                     /gene="skp"
                     /locus_tag="ACHJZ2_001128"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO4567286.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35385..36410
                     /gene="lpxD"
                     /locus_tag="ACHJZ2_001129"
     CDS             35385..36410
                     /gene="lpxD"
                     /locus_tag="ACHJZ2_001129"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO4567287.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36515..36970
                     /gene="fabZ"
                     /locus_tag="ACHJZ2_001130"
     CDS             36515..36970
                     /gene="fabZ"
                     /locus_tag="ACHJZ2_001130"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO4567288.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            36974..37762
                     /gene="lpxA"
                     /locus_tag="ACHJZ2_001131"
     CDS             36974..37762
                     /gene="lpxA"
                     /locus_tag="ACHJZ2_001131"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO4567289.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37762..38910
                     /gene="lpxB"
                     /locus_tag="ACHJZ2_001132"
     CDS             37762..38910
                     /gene="lpxB"
                     /locus_tag="ACHJZ2_001132"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO4567290.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            38907..39503
                     /gene="rnhB"
                     /locus_tag="ACHJZ2_001133"
     CDS             38907..39503
                     /gene="rnhB"
                     /locus_tag="ACHJZ2_001133"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO4567291.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            39540..43022
                     /gene="dnaE"
                     /locus_tag="ACHJZ2_001134"
     CDS             39540..43022
                     /gene="dnaE"
                     /locus_tag="ACHJZ2_001134"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO4567292.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43035..43994
                     /gene="accA"
                     /locus_tag="ACHJZ2_001135"
     CDS             43035..43994
                     /gene="accA"
                     /locus_tag="ACHJZ2_001135"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO4567293.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44093..46234
                     /gene="ldcC"
                     /locus_tag="ACHJZ2_001136"
     CDS             44093..46234
                     /gene="ldcC"
                     /locus_tag="ACHJZ2_001136"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO4567294.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46291..46680
                     /gene="yaeR"
                     /locus_tag="ACHJZ2_001137"
     CDS             46291..46680
                     /gene="yaeR"
                     /locus_tag="ACHJZ2_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4567295.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46745..48040
                     /gene="tilS"
                     /locus_tag="ACHJZ2_001138"
     CDS             46745..48040
                     /gene="tilS"
                     /locus_tag="ACHJZ2_001138"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO4567296.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(48093..48353)
                     /gene="rof"
                     /locus_tag="ACHJZ2_001139"
     CDS             complement(48093..48353)
                     /gene="rof"
                     /locus_tag="ACHJZ2_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO4567297.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(48340..48540)
                     /gene="yaeP"
                     /locus_tag="ACHJZ2_001140"
     CDS             complement(48340..48540)
                     /gene="yaeP"
                     /locus_tag="ACHJZ2_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO4567298.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48706..49251
                     /gene="yaeQ"
                     /locus_tag="ACHJZ2_001141"
     CDS             48706..49251
                     /gene="yaeQ"
                     /locus_tag="ACHJZ2_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO4567299.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49248..49658
                     /gene="arfB"
                     /locus_tag="ACHJZ2_001142"
     CDS             49248..49658
                     /gene="arfB"
                     /locus_tag="ACHJZ2_001142"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO4567300.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            49672..50382
                     /gene="nlpE"
                     /locus_tag="ACHJZ2_001143"
                     /gene_synonym="cutF"
     CDS             49672..50382
                     /gene="nlpE"
                     /locus_tag="ACHJZ2_001143"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO4567301.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(50582..51406)
                     /gene="yaeF"
                     /locus_tag="ACHJZ2_001144"
     CDS             complement(50582..51406)
                     /gene="yaeF"
                     /locus_tag="ACHJZ2_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO4567302.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(51459..53177)
                     /gene="proS"
                     /locus_tag="ACHJZ2_001145"
     CDS             complement(51459..53177)
                     /gene="proS"
                     /locus_tag="ACHJZ2_001145"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO4567303.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(53288..53995)
                     /gene="tsaA"
                     /locus_tag="ACHJZ2_001146"
     CDS             complement(53288..53995)
                     /gene="tsaA"
                     /locus_tag="ACHJZ2_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO4567304.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(53992..54396)
                     /gene="rcsF"
                     /locus_tag="ACHJZ2_001147"
     CDS             complement(53992..54396)
                     /gene="rcsF"
                     /locus_tag="ACHJZ2_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO4567305.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            complement(54514..55329)
                     /gene="metQ"
                     /locus_tag="ACHJZ2_001148"
     CDS             complement(54514..55329)
                     /gene="metQ"
                     /locus_tag="ACHJZ2_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO4567306.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(55369..56022)
                     /gene="metI"
                     /locus_tag="ACHJZ2_001149"
     CDS             complement(55369..56022)
                     /gene="metI"
                     /locus_tag="ACHJZ2_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO4567307.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(56015..57046)
                     /gene="metN"
                     /locus_tag="ACHJZ2_001150"
     CDS             complement(56015..57046)
                     /gene="metN"
                     /locus_tag="ACHJZ2_001150"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO4567308.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            57234..57809
                     /gene="gmhB"
                     /locus_tag="ACHJZ2_001151"
     CDS             57234..57809
                     /gene="gmhB"
                     /locus_tag="ACHJZ2_001151"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO4567309.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        13718 a        14335 c        15961 g        13932 t
ORIGIN      
        1 aaccctctcc cttaagggag aggggaccga tcgagctcaa attttgagag atatcttctt
       61 ctgtcttgta acagaagaac agaaaatcgg gcttttgcgc ctgaatattg cggagaaaaa
      121 gcatggccgg gaatgaccgc gagccaattg gacgcaaagg gaaaccgacg cgtccggtca
      181 aacaaaaggt aagccgtcgt cgttacgaag atgacgatga ttacgacgat tatgatgact
      241 atgaggatga agaaccgatg ccgcgcaaag gtaagggcaa aggcaaaggg cgtaagcctc
      301 gtggcaaacg cggctggcta tggctgctgt taaaactggc tatcgttttt gccgtgctga
      361 tcgccattta cggcgtttat ctcgatcaaa aaattcgtag ccgtattgat ggcaaggtct
      421 ggcaactgcc tgcggcagtt tatggtcgaa tggtcaacct tgagccagac atgaccatca
      481 gcaagaacga gatggtgaag ctgctggagg caacacagta tcgtcaggtg tcgaaaatga
      541 cccgtcctgg cgaatttacc gtgcaggcca acagcattga gatgattcgc cgtccgtttg
      601 atttcccgga cagtaaagaa gggcaggtgc gcgcgcgtct gacctttgat ggcgatcatc
      661 tggcgaccat cgtcaatatg gagaacaacc gtcagttcgg tttcttccgt cttgatccgc
      721 gtctgatcac catgatctcc tcaccaaacg gtgagcagcg tttgtttgtg ccgcgcagtg
      781 gtttcccgga tttgctggtg gatactttgc tggcgacaga agaccgccat ttctacgagc
      841 atgatggaat cagtctctac tccatcggac gtgcggtgct ggcaaacctg accgcaggtc
      901 gcacggtgca gggggcgagt acgctgacgc aacagctggt gaaaaacctg ttcctctcca
      961 gcgagcgctc ttattggcgt aaagcgaatg aagcctacat ggcgctgatc atggacgcgc
     1021 gttacagcaa agaccgtatt cttgagctgt atatgaacga ggtgtatctc ggtcagagcg
     1081 gcgacaacga aatccgcggc ttcccgctgg cgagcttgta ttactttggt cgcccggtag
     1141 aagaattgag cctcgatcag caggcgctgt tagtcggtat ggtgaaagga gcgtccatct
     1201 acaacccgtg gcgtaacccg aaactggcgc tggagcgacg taatctggta ctgcgtctgc
     1261 tgcaacagca acagattatt gatcaagaac tctatgacat gttgagtgcg cgtccgctgg
     1321 gggttcagcc gcgcggtggg gtgatctctc ctcagccagc ctttatgcaa ctggtgcgtc
     1381 aggagcttca ggctaaatta ggcgataagg taaaagatct ctccggcgtg aagatcttca
     1441 ctacctttga ctcggtggcc caggacgcgg cagaaaaagc cgccgtggaa ggtattccgg
     1501 cactgaagaa acagcgtaag ttgagcgatc ttgaaactgc gattgtggtc gtcgaccgct
     1561 ttagtggtga agttcgtgcg atggtcggag gttctgagcc gcagtttgcg ggctacaacc
     1621 gtgcgatgca ggcgcgtcgt tcgattggtt cccttgcaaa accagcgact tatctgacgg
     1681 ccttaagcca gccgaaaatc tatcgtctga atacgtggat tgcggatgcg ccaattgcgc
     1741 tgcgtcagcc gaatggccag gtctggtcac cgcagaatga tgaccgtcgt tatagcgaaa
     1801 gcggcagagt gatgctggtg gatgcgttga cccgttcgat gaacgtgccg acggtaaatc
     1861 tggggatggc gctggggctg cctgcggtta cggatacctg gattaaactg ggcgtaccga
     1921 aagatcagtt gcatccggtt ccggcaatgc tgctgggggc gttgaactta acgccaatcg
     1981 aagtggcgca ggcattccag accatcgcca gcggtggtaa ccgtgcaccg ctttctgcgc
     2041 tgcgttcggt aatcgcggaa gatggcaaag tgctgtatca gagcttcccg caggcggaac
     2101 gcgctgttcc ggcgcaggcg gcgtatctga cactatggac catgcagcag gtggtacaac
     2161 gcggtacggg tcgtcagctt ggggcgaaat acccgaacct gcatctggca gggaaaacag
     2221 ggactaccaa caataacgta gatacctggt ttgcgggcat tgacggcagc acggtgacca
     2281 tcacctgggt cggccgtgat aacaaccagc cgaccaaact gtatggagcc agcggggcaa
     2341 tgtcgattta tcagcgttat ctggctaacc agacgccaac gccgctgaat cttgttccgc
     2401 cagaagatat tgcagatatg ggcgtggact acgacggcaa ctttgtttgc agcggtggca
     2461 tgcgtgtctt gccggtctgg accagcgatc cgcaatcgct gtgccagcag agtgagatgc
     2521 agcagcagcc gtcaggcaat ccgtttgatc agtcttctca gccgcagcaa cagccgcaac
     2581 agcaacctgc tcagcaagag cagaaagaca gcgacggtgt agccggttgg attaaggata
     2641 tgtttggtag taattaacat ctaagcgtga aataccggat ggcgagttgc catccggtaa
     2701 aataacatcc cacccaaagt attaaccctt tcttttcatc tggttgttta ttaacccttc
     2761 aggaacgctc agattgcgta ccgcttgcga acccgccaga gtttcgaata ttatcttatc
     2821 tttataataa tcattctcgt ttacgttatc attcacttta catcagagat ataccaatgg
     2881 cgcgttccaa aactgctcag ccaaaacact cactgcgtaa aatcgcagtt gtagtagcca
     2941 cagcggttag cggcatgtct gtttatgcac aggcagcggt tgaaccgaaa gaagacacta
     3001 tcaccgttac cgctgcacct gcgccgcaag aaagcgcatg ggggcctgct gcaactattg
     3061 cggcgcgaca gtcagctacc ggcactaaaa ccgatacgcc gattcaaaaa gtgccacagt
     3121 ctatttctgt tgtgaccgcc gaagagatgg cgctgcatca gccgaagtcg gtaaaagaag
     3181 cgcttagcta cacgccgggt gtctctgttg gtacgcgtgg cgcatccaac acctatgacc
     3241 acctgatcat tcgcggtttt gcggcagaag gccaaagcca gaataactat ctgaatggcc
     3301 tgaagttgca gggcaacttc tataacgatg cggtcattga tccgtatatg ctggaacgcg
     3361 ctgaaattat gcgtggcccg gtttccgtgc tttacggtaa aagcagtcct ggcggcctgt
     3421 tgaatatggt cagcaagcgt ccgaccaccg aaccgctgaa agaagttcag tttaaagccg
     3481 gtactgacag cctgttccag actggttttg actttagcga tgcgctggat gatgacggcg
     3541 tttactctta tcgcctgacc ggtcttgcgc gttctgccaa tgcccagcag aaagggtcag
     3601 aagagcagcg ttatgctatt gcaccggcgt tcacctggcg tccggatgat aaaaccaatt
     3661 tcaccttcct ttcttacttc cagaacgagc cggaaaccgg ttattacggc tggttgccga
     3721 aagagggaac cgttgagccg ctgccgaacg gtaagcgtct gccgacagac tttaacgaag
     3781 gggcgaagaa caacacctat tctcgtaatg agaagatggt gggctacagc ttcgatcacg
     3841 aatttaacga cacctttact gtgcgtcaga acctgcgctt tgctgaaaac aaaacctcgc
     3901 aaaacagcgt ttatggttac ggcgtctgct ccgatccggc gaatgcttac agcaaacagt
     3961 gtgcggcatt agcgccagcg gataaaggcc attatctggc acgtaaatac gtcgttgatg
     4021 atgagaagct gcaaaacttc tccgttgata cccagttgca gagcaagttt gccactggcg
     4081 atatcgacca caccctgctg accggtgtcg actttatgcg tatgcgtaat gacatcaacg
     4141 cctggtttgg ttacgacgac tccgtaccgc tgctcgatct gtacaatccg gtgaataccg
     4201 atttcgactt caatgccaaa gatccggcaa actccggccc ttaccgcatt ctgaataagc
     4261 agaaacaaac gggcgtttat gttcaggatc aggcgcagtg ggataaagtg ctggtcaccc
     4321 tgggcggtcg ttatgactgg gcagatcaag aatctcttaa ccgcgttgcc gggacgaccg
     4381 ataaacgtga tgacaaacag tttacctggc gtggtggtgt taactacctg tttgataatg
     4441 gcgtaacacc ttactttagc tatagcgaat cgtttgaacc ttcttcgcaa gttgggaagg
     4501 atggtaatat tttcgcaccg tctaaaggta agcagtatga agtcggcgtg aaatatgtac
     4561 cggaagatcg tccgattgta gttactggtg ccgtgtataa tctcactaaa accaacaacc
     4621 tgatggcgga ccctgagggt tccttcttct cggttgaagg tggcgagatc cgcgcacgtg
     4681 gcgtagaaat cgaagcgaaa gcggcgctgt cggcgagtgt taacgtagtc ggttcttata
     4741 cttacaccga tgcggaatac accaccgata ctacctataa aggcaatacg cctgcacagg
     4801 tgccaaaaca catggcttcg ctgtgggctg actatacctt ctttgacggt ccgctttcag
     4861 gtctgacgct gggcaccggt ggtcgttata ctggctccag ctatggtgat ccggctaact
     4921 cctttaaagt gggaagttat acggtcgtgg atgcgttagt gcgttatgat ctggcgcgag
     4981 tcggcatggc gggctccaac gtggcgctgc atgttaacaa cctgttcgat cgtgaatacg
     5041 tcgccagctg ctttaacact tatggctgct tctggggcgc agaacgtcag gtcgttgcaa
     5101 ccgcaacctt ccgtttctaa tttctctttt ggggcacgga tttccgtgcc tgtttcacaa
     5161 gttggctgtt atgcaggaat acacgaatca ttccgatacc acttttgcac tgcgtaatat
     5221 ctcctttcgt gtgcccgggc gcacgctttt gcatccgctg tcgttaacct ttcctgccgg
     5281 gaaagtgacc ggtctgattg gtcacaacgg ttctggtaaa tccactctgc tcaaaatgct
     5341 tggccgtcat cagccgccgt cggaagggga gattcttctt gatgcccagc cgctggaaag
     5401 ctggagcagc aaagcgttcg cccgcaaagt ggcttatttg ccgcagcagc ttcctccggc
     5461 agaagggatg accgtgcgtg aactggtggc gattggtcgt tacccgtggc atggcgcact
     5521 agggcgcttt ggtgcggcag atcgcgaaaa agttgaggaa gctatctcgc tggttggctt
     5581 aaaaccgctg gcgcatcggc tggtagacag cctctctggc ggcgaacgtc agcgggcgtg
     5641 gatcgccatg ctggtggcgc aggatagccg ttgtctgttg ctcgacgaac cgacttcggc
     5701 gttggatatc gcccaccagg ttgatgtgct ggcgctggtg caccgtttaa gtcaggagcg
     5761 tggcctgacg gtcattgccg tgctgcacga tatcaatatg gctgcgcgtt actgtgatta
     5821 tctggtcgcc ctgcgcggcg gtgaaatgat tgctcaggga acgcctgcgg aaattatgcg
     5881 cggcgaaacc ctcgaaatga tttatggcat cccgatgggt attctgccgc atccggcggg
     5941 tgctgcacct gtgagttttg tttattgatg agcggcttac ctcttatttc gcgccgtcga
     6001 ctgttaacgg cgatggcgct ttctccgttg ttatggcaga tgaataccgc ccacgcggcg
     6061 gctattgacc caaatcgtat tgtggcgctg gagtggctgc cggtggaatt actactggcg
     6121 ctcggcatcg tgccttatgg cgtggcagac accatcaact atcgactgtg ggtcagcgaa
     6181 ccaccactac ctgaatcggt gattgacgtc ggtttgcgta cagaacctaa cctcgaactg
     6241 ctgaccgaaa tgaaaccgtc gtttatggtc tggtcggcgg gatatggtcc ttcgccagaa
     6301 atgctggctc gtattgcgcc ggggcgtgga tttaacttca gcgacggcaa acagccgctg
     6361 gcgatggcgc gtaaatcgct gacggaaatg gcggatttac ttaacctgca aagcgcagca
     6421 gaaacgcatt tagcgcaata tgaagacttt atccgcagca tgaacccccg ctttgtgaag
     6481 cgtggtgcgc gtccgttatt gctgacgacg cttatcgatc cgcgccatat gctggtcttc
     6541 ggtccaaaca gcttgttcca ggaaattctt gatgagtacg gcatcccaaa tgcctggcaa
     6601 ggggaaacca acttctgggg cagtaccgcc gtcagcatcg atcgtctggc agcgtataaa
     6661 gacgttgatg tgctctgttt tgatcacgac aacagcaaag acatggatgc gctaatggca
     6721 acgccgctgt ggcaggccat gccgtttgtc cgcgccggac gctttcagcg cgtacccgca
     6781 gtctggtttt atggtgcaac tctctcggca atgcactttg tgcgcgttct ggataacgcc
     6841 attggaggta aagcgtgagt aaacgtattg cgcttttccc ggcattattg ctggcgttgc
     6901 tggtgattgc cgctacggcg ctgacctgga tgaacttctc gcaggcgctg ccgcgtagcc
     6961 agtgggcgca ggctgcctgg tcgccggata ttgacgtcat cgagcagatg atttttcact
     7021 acagcttgct gccgcgtctg gcgatttcgc tgctggtggg cgcaggcctg gggctggtgg
     7081 gcgtgctgtt tcagcaagtg ctgcgtaatc cgctggcgga accgacgaca cttggcgttg
     7141 ctacaggcgc gcaactgggg attaccgtca ctacgctctg ggcgatccca ggggcgatgg
     7201 cgagtcagtt tgctgcgctg gcgggcgctt gcgttgttgg cttaatcgtc tttggcgtcg
     7261 cgtgggggaa acggctttcg ccggtaacgc tgatcctcgc ggggctggta gtgagccttt
     7321 attgtggggc aatcaatcag ttactggtta tcttccacca tgaccaactg caaagcatgt
     7381 tcctgtggag caccggaacg ctgacgcaaa ccgactgggg cggtgttgag cgtttatggc
     7441 cgcagttgct gggcggtgtg atgctgacgt tgctgctact tcgtccgtta acactgatgg
     7501 ggcttgatga tggcgtggcg cgcaatctcg ggctagcctt gtcgctggcg cgtctggcgg
     7561 cgctgtcgct ggctattgtc atcagtgcgc tgctggtgaa cgcggtgggg attatcggct
     7621 ttatcgggtt gttcgcgccg ttgctggcaa aaatgctggg ggcgcggcgt ctgctgccgc
     7681 gactgatgct ggcgtcgttg attggtgcgc tgatcctctg gctttccgat caaatcatcc
     7741 tctggctgac tcgcgtgtgg atggaagtgt ccaccggttc ggtcactgcg ttgatcggtg
     7801 cgccgctgct actgtggctg ttgccgcgtt tacgcagcat tagcgcgccg gatatgaagg
     7861 tcaacgatcg tgtcgcggct gaacgccaac atgtgctggc gtttgccctc gcgggcggcg
     7921 tgctgctgtt gatggctgtg gtggtggcgc tgtcgtttgg tcgtgatgcg cacggctgga
     7981 cgtgggcgag cggggcgttg ctcgaggatt tgatgccctg gcgctggccg cgaattatgg
     8041 cggcgctgtt tgcgggcgtc atgctggcgg tggcgggctg tattattcag cggctgaccg
     8101 gaaacccgat ggcaagcccg gaagtgctgg ggattagctc tggcgcggcg tttggcgtgg
     8161 tactgatgct gtttctggta ccgggtaatg cttttggctg gctgttaccc gcgggaagta
     8221 tcggtgcggc ggtgacgctg ttgattatta tgatcgccgc cgggcgcggt ggattttccc
     8281 cacaccgcat gttactggcg gggatggcgt taagtaccgc attcaccatg cttttaatga
     8341 tgttgcaggc gagtggtgac ccgcgaatgg cgcaagtgct gacctggatc tctggttcga
     8401 cctacaacgc caccgatgcg caggtctggc gcaccggaat tgtgatggtg attttgctgg
     8461 cgattacccc gctgtgccgc cgctggctga ccattttacc gctgggtggt gataccgcgc
     8521 gggccgtggg ggtggcgtta acgccgacgc gaattgcgct gctgctgtta gcggcttgcc
     8581 tgacggcgac cgcgacgatg accattggac cgttgagttt tgttggttta atggcaccgc
     8641 atatcgcgcg gatgatgggc tttcgacgga cgatgccgca catcgtgatt tcggcgctgg
     8701 tgggtggttt actgctggtg ttcgctgact ggtgtgggcg gatggtgctg tttccgttcc
     8761 agatcccggc ggggctgctg tcgaccttta tcggcgcgcc atattttatc tatttgttga
     8821 gaaagcagag ccgttaataa aatgcctcgc ccggtgggcg aggcaaaatc atcacaactt
     8881 cgcaaacacc cgacgtgcag catcgatggt gttattgata tcttccatgc tgtgcgccac
     8941 agacataaag cccgcttcaa acgctgacgg tgccaggtaa acaccttcat ccagcatcat
     9001 atggaagaaa cgcttaaaac gctcgacgtc acaggccatc acatcctgat agcacgtcac
     9061 ggactcggcg tcggtaaaga aaataccgaa catgccgcca acgtggttta ccaccagcgg
     9121 aattccggct tcttctgccg cttccagcag accttctgcc agacgtgttg tcagctcatc
     9181 cagcgtttcg tgaacgcccg gctgcgcgac ttcattcaga caggcgaaac cggctgccat
     9241 cgcaatcggg ttaccggaaa gcgtacccgc ctgatagacc ggacccgtcg gggccagcgc
     9301 atccattaca tcacgacgac caccgaatgc gcctaccggc attccaccgc cgatgatttt
     9361 gcccaggcaa gtgagatccg gttccacacc gtaataatcc tgtgcgccag ctagcgctac
     9421 gcggaaaccg gtcattactt catcgatgat cagcaacgcg ccaaattcgt cgcacagcgc
     9481 gcgcagacct ggcaggaagt ctggcagcgg tggaacacag ttcatattgc ctgccaccgg
     9541 ctcgacgata atacaggcaa tctcttgcgg gtattgctca aacgcggcgc gtacagaagc
     9601 cagatcgtta taagtacagg ttaaggtatg tttggcgaaa tctgccggaa cgcccggcga
     9661 gtttggctgg cctaacgtga gtgcgccaga gccggctttc accagcaggc agtcagcgtg
     9721 accgtggtaa caaccttcaa atttaataat tttgtcgcga ccggtaaaac cacgggccag
     9781 gcggatggcg ctcattgtcg cttcagtgcc ggagttcacc atgcgcacca tatccatggt
     9841 cgggaccagt tcggtcacca gttgcgccat tttcacttcc atttcggttg gtgcaccaaa
     9901 gcttaaacca cgctcggcgg cttcaatcac ggcattgcgg atcgccggat ggttatggcc
     9961 cagcaccatc ggcccccagg aaccgacata atcgatatag gctttgccat caacatcgta
    10021 cagataagcg ccgtccgctt tttcgataaa cagtggagtg ccgcccacgc cagtaaaggc
    10081 gcgaacaggg gagttcacac cgccagggat cagctcgcgc gctgcgctgt aaagattttc
    10141 agacttactc atggagggtt cctgattcgt agaaaaagtg aatggctgct attctatgtt
    10201 attcataaca agttaaatac tcgtcaaaca tcaggctgct tgtactggtg caatcctgat
    10261 ttcttagagt ataaaagttt tgtgcatttg aaacattacg ctttgcaaag gattttcatg
    10321 gaacgtgcga gtaaaatgcc gtcatcttat ttgtatgacc aataagtgat cattggatga
    10381 aaactgatac tccctcttta gaaacaccgc aggccgcgcg cctgcgacgc agacaactga
    10441 ttcgccaact tcttgagcgc gataaaaccc cgttagccat tttgtttatg gcggcagtcg
    10501 tcggcacgct tgtcgggctg gcagcggttg cttttgacaa aggtgtcgcc tggttgcaga
    10561 accaacgtat gggggcgctg gtacatactg ctgataatta tccgctactg ttaaccgttg
    10621 cttttctctg ttcggcggtg ctggcgatgt tcggctactt tttggtgcgc aaatacgcgc
    10681 ccgaagcagg ggggtcgggg atcccggaaa ttgaaggggc gctggaagat caacgtcctg
    10741 ttcgctggtg gcgtgtattg ccggtgaaat tctttggcgg gctggggaca ctcggcggag
    10801 gcatggtgtt ggggcgcgaa gggccaaccg tgcagatcgg cggtaatatc gggcggatgg
    10861 cactcgatgt tttccgcctg aaaggtgatg aagctcgcca tacgctgttg gcaaccggtg
    10921 cggcggcggg tctggcggcg gcctttaacg cgccgctggc gggtattttg tttattatcg
    10981 aagagatgcg tccgcagttt cgctatacgt taatttcgat taaagcggta tttattggcg
    11041 tcattatgtc gaccattatg taccggattt ttaatcatga agttgcgttg attgacgtcg
    11101 gtaaactttc tgacgcgccg cttaatacgc tgtggcttta tctgatcctc ggtattattt
    11161 ttggcatttt cggccctatt tttaataaat gggtgctggg gatgcaggat ttgctgcacc
    11221 gtgtgcacgg cggcaatatt accaaatggg tactgatggg tggtgcgatt ggcggtctgt
    11281 gtggattgct ggggtttgtg gcaccagcaa cgtcgggcgg cggttttaac ctgattccta
    11341 tcgctaccgc ggggaatttc agcatgggaa tgctggtgtt tatcttcgtc gcgcgggtca
    11401 ttaccacctt actctgcttc tcttccggcg cgccgggcgg tatttttgcc ccgatgctgg
    11461 cgctgggtac tgtgctggga accgctttcg gaatggttgc cgttgagctg tttccgcaat
    11521 atcaccttga ggcggggacg tttgctattg ccggaatggg ggcgttactg gcggcatcta
    11581 ttcgcgcgcc gttaacgggg atcattctgg ttctggagat gaccgataac taccagctca
    11641 ttttgccaat gattattacc ggtcttggcg caacactatt agcgcaattt accggcggga
    11701 aaccgctata ctcggcgatt cttgcgcgca cgctggcaaa acaggaagct gagcaactgg
    11761 cgcgaagcaa ggccgcatca gccagcgaga atacttgaac gaaataccag ggtattagat
    11821 aatggcgatt attattgggt tagaatttgc ccaattgccg atgtcgtttg gagcaaaata
    11881 tgagtgatga cgtagcactg ccgctggagt ttaccgacgc agcagccaac aaagttaaaa
    11941 gcctgatcgc tgacgaagat aacccgaatc tgaaattacg cgtgtatatc accggtggcg
    12001 gttgcagcgg cttccagtat ggtttcacct ttgatgatca ggtgaacgaa ggcgatatga
    12061 ccatcgaaaa acagggcgtt ggcctggtgg ttgatccgat gagcctgcaa tatctggtcg
    12121 gcggttccgt tgactatacc gaaggactgg aaggttctcg tttcatcgtg accaacccga
    12181 acgcgaaaag cacctgcggt tgcggttctt cctttagtat ctaatctgtt gttcatgtgc
    12241 cggataaact atccggcaca tcttctctct tcaacgccca ttctcatcca gcgcaaacgt
    12301 cggtagctta agatgccaac gaatagccgc cagccgaatc aatagcgtca cgaccatgcc
    12361 catcatactg gctgtttcca gtggtacgga aaatgtgtaa taagccgtag cgtggacaat
    12421 accgccgata atacaggcag ttgcgtagat ttctgtacgt aaaatcatgg ggatttcgcg
    12481 ggccagaaca tcacgaatga tcccgccgcc aacgccagta atgacgccca tacaaaccgc
    12541 gattaacgga ccggcttccg cattaaaggc tttattcacg ccaatgccga caaacaccgc
    12601 cagaccaacg gcgtccagca ccggcaacat ccattttggt aagtgtcttg gctggcgcac
    12661 cagcacgatg gtcagcatgc tggttaccat tgcaacgacc agatcggtgg gatctttcac
    12721 ccaaaatacc gggccgtgat ccagcgccat gtcgcgaatt gtcccgccgc ctacagcggt
    12781 aaccacgccc agtaccagaa caccaaaagg gtccatacgc aattttccgg ctaacaaaac
    12841 gccggagatg gcaaataccg ctgtgccgac tatatccagc caatagacga gcattgttca
    12901 atccccactg agcacctaaa aaggtcaggc gctaatctac ctgtgaaagc gcattacaga
    12961 gctgttgtgc agcgaggata atacgtgggc ttgcacgttc aaaccagtca ctcgtgagag
    13021 gaataacggg aattttgagc tgttcacccc agtattgttt gattttagga atttggtccg
    13081 gtccgcctgt aatgacaatc gcctgtggcg agcgtgctaa cacctgttcg cggctaactt
    13141 gcggccaggg aacccggctg tctttaaaga tgttttctcc gccacaaact tcgagtacct
    13201 ggttttgaat cgactctttt ccactggtaa atggcggatt aatgccgaat tgcagaaaaa
    13261 cacgtttttt aggtttatca gcatattgcg ctttcaattg cgcgtattga tccagcaggg
    13321 attgcgcggc ttgttcggcc ttatccggtt gcggactcca gggagccagt tgacgtaacg
    13381 cattggcaat ttgttcaatg tttgtcgcat cgacccacat cacttttatt cccagcgaag
    13441 ccagctggtc aacctgccgc tcggcattac ctccacgcca ggcaatcacc agatcgggtt
    13501 tcagcgcgac aatgcgttcc agattcatcc cctgccaggt ggaaacctgc tcaatctttt
    13561 gcgcttgtgg aggatagtcg gaatagctgc tgaccccaac cggcgtgatc ccggcggcaa
    13621 aggcaagttc agtgttggcg ggagaaagcg tgatgacgcg cggcgcggcg ttgagccaca
    13681 gtggcgcaag aaaagacagg gcgaccagcg ccctgaacag tgacttagcc atgtgccagt
    13741 ttctgcacta gtgactcaac catcaggctg gactgtttag cggcaacagc caggaactca
    13801 tcgaagctaa gatgagactg ttgatcggcc acgtcggaga tggcgcgtac gacaacaaac
    13861 gggacgttga aattgtggca gacatgggcg attgccgtcg cttccatctc tacagcaatg
    13921 gcctgtggga agttgtggcg gattttcgcc agaccaacag aaccgttgat gaaagcgtcg
    13981 ccgctaacaa tcaggccacg tacagcgtta agattcagtt cggcaatgca ggcctcagcg
    14041 gcagcgatca gtttatcgtc agctttaaag cctgccggac agcctggtaa ctgaccgtat
    14101 tcataaccaa atgccgtgac atccgcgtcg tgataacgcg cttcgtccga gacaacgata
    14161 tcgcccactt tcagcgttgg tgccaggcca ccggcagaac cggtgttaat aatcacatct
    14221 ggcttgcagt gttccaacag cagagtggca cccagcgccg cagcgacttt accgatgccc
    14281 gatttcagaa gcgcaacctc ggttccattc agttggccgg tatagatttc gcaaccgccc
    14341 agactgatag tttgacggtt ttcgattttg tcacgcagca gcgtaacttc ttcttccatt
    14401 gcaccaatga tgccgatttt catagattta ctcgcgataa gcccgatttg aaggcatagt
    14461 ttaccatgcg cttacgggga agcgtatttc tcacgcggga gaggacatgg cacagattga
    14521 tttccgaaaa aaaataaact ggcatcgtcg ttaccgttca ccgcagggcg ttaaaaccga
    14581 acatgagatc ctgcggatct tcgagagcga tcgcgggcgt atcatcaact ctccggcaat
    14641 tcgtcgtctg caacaaaaga cccaggtttt tccactggag cgcaatgccg ccgtgcgcac
    14701 gcgtcttacc cactcgatgg aagtccagca ggtggggcgc tacatcgcca aagaaatttt
    14761 aagccgtctg aaagagctta aattgctgga agcatacggc ctggatgaac tgaccggacc
    14821 ttttgaaagc attgttgaga tgtcatgcct gatgcacgat atcggcaatc cgccgtttgg
    14881 tcattttggc gaagcggcga taaatgactg gtttcgccag cgtttgcacc cggaagatgc
    14941 cgaaagccag cctctgactg acgatcgctg cagcgtggcg gtactacgtt tacgggacgg
    15001 ggaagaaccg cttaacgagc tgcggcgcaa gattcgtcag gacttatgtc attttgaggg
    15061 gaatgcacaa ggcattcgtc tggtgcatac attgatgcgg atgaatctca cctgggcgca
    15121 ggttggcggt attttaaaat atacccgtcc agcgtggtgg cgtggcgaaa cgcctgagac
    15181 acatcactat ttaatgaaaa agccgggtta ttatctttct gaagaagcct atattgcccg
    15241 gttgcgtaaa gaacttaatt tggcgcttta cagtcgtttt ccattaacgt ggattatgga
    15301 agctgccgac gacatctcct attgtgtggc agatcttgaa gatgcggtag agaaaagaat
    15361 atttaccgtt gagcagcttt atcatcattt gcacgaggcg tggggccagc atgagaaagg
    15421 ttcgctcttt tcgctggtgg ttgaaaatgc ctgggaaaaa tcacgctcaa atagtttaag
    15481 ccgcagtacg gaagatcagt tttttatgta tttacgggta aacaccctaa ataaactggt
    15541 accctacgcg gcacaacgat ttattgataa tctgcctgcg attttcgccg gaacgtttaa
    15601 tcatgcatta ttggaagatg ccagcgaatg cagcgatctt cttaagctat ataaaaatgt
    15661 cgctgtaaaa catgtgttta gccatccaga tgtcgagcag cttgaattgc agggctatcg
    15721 ggtcattagc ggattattag agatttatcg ccctttatta agcctgtcat tatcagactt
    15781 tactgaactg gtagaaaaag aacgggtgaa acgttcccct attgaatctc gcttattcca
    15841 caaactctcg acgcgccatc ggctggccta tgtcgaggct gtcagtaaat taccgtcaga
    15901 ttctcctgag tttccgctat gggaatatta ttaccgttgc cgcctgctgc aggattatat
    15961 cagcggtatg accgacctct atgcctggga tgaataccga cgtctgatgg ccgtagaaca
    16021 ataaccaggc ttttgtaaag acgaacaata aatttttacc ttttgcagaa actttagttc
    16081 ggaacttcgc gctataaaac gaatctgaag aacacagcaa ttttgcgtta tctgttaatc
    16141 gagactgaaa tacatgaaaa aaaccacatt agcactgagt gcactggctc tgagtttagg
    16201 tttggcgtta tctccgctct ctgcaacggc ggctgagact tcttcagcaa cgacagccca
    16261 gcagatgcca agccttgcac cgatgctcga aaaggtgatg ccttcagtgg tcagcattaa
    16321 cgtagaaggt agcacaaccg ttaatacgcc gcgtatgccg cgtaatttcc agcagttctt
    16381 cggtgatgat tctccgttct gccaggaagg ttctccgttc cagagctctc cgttctgcca
    16441 gggtggccag ggcggtaatg gtggcggcca gcaacagaaa ttcatggcgc tgggttccgg
    16501 cgtcatcatt gatgccgata aaggctatgt cgtcaccaac aaccacgttg ttgataacgc
    16561 gacggtcatt aaagttcaac tgagcgatgg ccgtaagttc gacgcgaaga tggttggcaa
    16621 agatccgcgc tctgatatcg cgctgatcca aatccagaat ccgaaaaacc tgaccgcaat
    16681 taagatggcg gattctgatg cactgcgcgt gggtgattac accgtagcga ttggtaaccc
    16741 gtttggtctg ggcgagacgg taacttccgg gattgtctct gcgctggggc gtagcggcct
    16801 gaatgccgaa aactacgaaa acttcatcca gaccgatgca gcgatcaacc gtggtaactc
    16861 cggtggtgcg ctggttaacc tgaacggcga actgatcggt atcaacaccg cgatcctagc
    16921 accggacggc ggtaacatcg gtatcggttt tgctatcccg agtaacatgg tgaaaaacct
    16981 gacctcgcag atggtggaat acggccaggt gaaacgcggt gagctgggta ttatggggac
    17041 tgagctgaac tccgaactgg cgaaagcgat gaaagttgac gcccagcgcg gtgctttcgt
    17101 aagccaggtt ctgcctaatt cctccgctgc aaaagcgggc attaaagcgg gtgatgtgat
    17161 cacctcactg aacggtaagc cgatcagcag ctttgccgca ctgcgtgctc aagtgggtac
    17221 tatgccggtg ggcagcaaac tgaccctggg cttgctgcgc gacggtaagc aggtcaacgt
    17281 gaatctggaa ctgcagcaga gcagccagaa tcaggttgat tccagctcca tcttcaacgg
    17341 cattgaaggc gctgagatga gcaacaaagg caaagatcag ggcgtggtag tgaacaacgt
    17401 gaaaacgggc actccggctg cgcagatcgg cctgaagaaa ggtgatgtga ttattggcgc
    17461 gaaccagcag gctgtgaaaa acatcgctga actgcgtaaa gttctcgaca gcaaaccgtc
    17521 tgtgctggca ctgaacattc agcgcggcga cagcaccatc tacctgttaa tgcagtaatc
    17581 tccctcaacc ccttcctgaa aacgggaagg gggtctcctt acaatctgtg aacttcacca
    17641 caactccata catcttcatc atcctttagg catttgcaca atgccgtacg ttacgtactt
    17701 ccttatgcta agccgtgcat aacggaggac ttatggctgg ctggcatctt gataccaaaa
    17761 tggcgcagga tatcgtggca cgtaccatgc gcatcatcga taccaatatc aacgtaatgg
    17821 atgcccgtgg gcgaattatc ggcagcggcg atcgtgagcg tattggtgaa ttgcacgaag
    17881 gtgcattgct ggtactttca cagggacgag tcgtcgatat cgatgacgcg gtggcacgtc
    17941 atctgcacgg tgtgcggcaa gggattaatc taccgttacg gctggaaggt gaaattgtcg
    18001 gcgtaattgg cctgacaggt gaaccagaga atctgcgtaa atatggcgaa ctggtctgca
    18061 tgacggctga aatgatgctg gaacagtcgc ggttgatgca cttgttggcg caggatagcc
    18121 gtttgcggga ggaactggtg atgaacctga ttcaggcaga ggagaatact cccgcactta
    18181 ctgaatgggc gcaacggctg gggatcgatc tcaatcaacc gcgagtggtg gctattgttg
    18241 aggtcgacag cggtcagctt ggtgtggaca gcgcaatggc ggagttacaa caactgcaaa
    18301 acgcgctgac tacgcccgag cgtaataatc tggtggcgat tgtctcgcta accgaaatgg
    18361 tggtgttgaa accggcgttg aactcttttg ggcgctggga tgtagaagat catcgtaagc
    18421 gagttgaaca actgattacc cgcatgaaag agtacggcca gctgcgtttt cgcgtttcac
    18481 tgggcaacta ttttaccggt cctggcagta ttgcccgatc ctatcgtacg gcgaaaacga
    18541 cgatggtggt gggtaaacag cggatgccag aaagtcgctg ctatttttat caggatctga
    18601 tgttacctgt gttactcgac agtttgcgtg gcgactggca ggccaacgaa ctggcgcgac
    18661 cgctggcgcg gctgaaagcg atggacaata acggcttgct gcgacgaacg ctggcggcgt
    18721 ggtttcgtca caatgtgcaa ccgctggcaa cgtcaaaggc gttgtttatt catcgtaata
    18781 ccctggagta tcggcttaat cgtatatcgg aactgaccgg gcttgatttg ggcaattttg
    18841 atgacaggtt gctgctgtat gtggcgttac aactggatga agagcggtag gttatgcgtt
    18901 aaggttgagg cggcgctggc tcattcccct cagcccgaag gggagagggg aatgcccgag
    18961 ttaaccgtca attcgggatt atttacgcgt tagtttttcc agatcggctt caatctcgct
    19021 gatcttattg gttacgactg actccaggtg acgtaagtcg tcgaggatct tacgcttaag
    19081 atcaacttcg ctgcggtcac gctggcagat ttgatcaagc tcatcaatga tataccgtag
    19141 attcgggctg atttcctgga cttctttata accctgaccc acaccatcag cgacgaccgt
    19201 tttacgctga cgcggatatt taaacttaac gctcttggcg aaaaactcgc ctttgtcttt
    19261 ctggaaatag attttcagaa tatcgttgtt ggcttcctgc cggaggctgt aacgatcaat
    19321 ttcttcagga ttggtaatac ccagactttt cagattgtcg tacatagcgt tacctcaaaa
    19381 tgagtcagta aaatgtgctt attttagcat ttggcctggc ccgcccggct cgactgtgat
    19441 cgcagaaagc ctttttcttc cagacttcta tcatggcgta aaaatcaaaa aattacctgc
    19501 tttattgtgg cgatattcca tgggatggat tagcacattt atcttcgttt tgcgaattac
    19561 atgacagtcc gaaacaagat gaaaaagtat ttaaaaaagg ggggaggaag aatgagtagg
    19621 acgaattatt ctgaaccaga aagcaatcgg gcagcgtcgt gctgcccgtg tgcatacttt
    19681 tagtcgatgg tacgcagcag ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact
    19741 ttcttaacga taaccgcaca gtagaggctg tatttgccat ctttcgacgg cagattacct
    19801 gaaacaacca cagaccccgc cggaacgcga ccgtagtgga tgtcgccggt ttcacggtcg
    19861 taaatacggg tgctctgacc aatgtatacg cccatggaaa tgacggaacc ttcttcgaca
    19921 atcacccctt caactacttc agagcgcgcg ccgatgaagc aattatcttc aatgatggtt
    19981 gggttagcct gcagcggttc cagcacaccg ccgatgccca cgccaccgga aaggtggacg
    20041 tttttaccaa tctgcgcaca agaaccgacg gtcgcccagg tatcgaccat ggtgccttca
    20101 tcaacatatg cgccgatgtt gacgtaagac ggcatcagca cggtgttacg ggcaataaac
    20161 gcaccctgac gtaccgccgc aggtggtaca acgcggaagc cttctttctg gaaacgtgct
    20221 tcgtcgtagt cggcgaattt catcggcact ttgtcgaagt agcggctttc tgccccttcg
    20281 atcacctgat tatcattaat acggaaagag agcagcaccg cttttttcaa ccactgatgc
    20341 gtcacccact gaccgtcaat tttttccgct acacgcagtg cgccggaatc cagcagggcg
    20401 atcacctgat ttaccgcttc gcgggtaacg gtgtctgcat tggctggcgt gatctcggca
    20461 cggcgttcaa aagcggtttc aataatgttc tgtaactgct gcattgttaa actcttttca
    20521 tatcagtaaa cacatcaccc tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc
    20581 acttcctgct gcaactcgtt attaagtgca cgccggtcag cggtggcaat tatgaataaa
    20641 tcttctactc gctcgccaat ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg
    20701 gcaaaaattt tcccgactct cgccagcagt cctggctggt cgagcgcgat aagctcaagg
    20761 aacgatttgc ggtcggtatg ggttggcaaa aacgtcactt cggtttcaac agtaaagtga
    20821 cgtagtttag ccggttgacg acgcggctgt ggtggctgcc agctactttg tgtcagtact
    20881 tgctccagac caaaccgaat aacctcatga cgatctgcgg atagcgggct accatcgggt
    20941 tccagcacga taaaggtatc catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg
    21001 tgaacgctta agttgcgtct atctaactcg gcacaaacag cagcaaacaa gtaagggcgg
    21061 tccgggctcc agataaaaat ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc
    21121 agcggtttgc ttaaatcatg ctgtaataaa tggcgcgcat gccaggccag ttgatttggg
    21181 ctatgacgga caaaatagtt cgcgcgacag cgtgaccaaa tttggtgcag cacctcttcg
    21241 tcgatgttat ccatgcgcag tagtgccagc gcctggagtt ggtgatggcg aacccgttcg
    21301 cgcatatccg gcgtgttttg cattccgcgt cgtagctgct tttcggtggc gaaatagagt
    21361 tcgcgcaaca gactttgctt ccagctattc cacagcgttt cgttggtggc gcaaatgtca
    21421 gccactgtca ggcataccag atagcgcaga cgattttccg tttgaacttc ttcggcaaac
    21481 tgcttgatga cttccgggtc ctgaatatcg cgacgctgag ctgtcaccga catcagcaag
    21541 tgctggcgaa ccagccaggc gaccagctgt gtttcgcgtg agttcagccc gtggagttcg
    21601 gcaaaatgca ctacatcctg agcaccgaga atagagtggt cgccgccgcg tcctttggcg
    21661 atatcgtgaa acagcgcggc gatgaaaatc agctcagttg acggtaggcg cggccagacg
    21721 ttcacacaca acggatggcg ctggcgcgtt tcttcactgg caaaactctc cagtttcagc
    21781 atcacgcgga tagtatgttc atccaccgtg taggcgtgga acagatcaaa ctgcatctgc
    21841 ccgacgatat gtgaccattg cggcatatac gcgccgagta cgctatggcg atgcattggc
    21901 aatagcccgc gccgcaccgc tccggggtga cgtagaatgc tcaaaaacag ttttcgtgct
    21961 tgcggaatat tacacagcgg ttgttgcaga tggcgacggg catggcgtaa ctggcgcagt
    22021 gtggtggagt aaatgccggt gatcgcactg ttgcgcacca tggtgtagaa catacgcaag
    22081 atggcttccg gctggcgcat aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt
    22141 agctgaaact catcgtcgat tggacgtggt ttttcgtcgg cgggaagggc gaggatggct
    22201 tcatcgaaca gttgcagcag catctggttg agttcactga cgcggcgtgt cacgcggaag
    22261 taatccttca tcatccgctc gaccggttcg ttaccttcgc cactgtaatt cagacgctgg
    22321 gcgacgctaa gctggcgatc gaataacagg cgattatcgt aacggctgac gaccagatgc
    22381 agggcaaagc gaatacgcca caatatatgc agacattcgt ttaattccgc ccgctccgct
    22441 gaggttaaga agccaaaccc aaccatttca tccagcgatg ttgcgccaaa atgacggcgg
    22501 gccacccatt gcagagtgtg gatatcgcgc aagccgccag ggctactttt gatgtctggt
    22561 tcaagattgt agctggtgcc gtggtaacgc tgatggcgct ggttctgttc ttcaactttc
    22621 gccgcgtaga acttgtcgga aggccagaat ccttcgctga aaatatgttt ttgcagttca
    22681 agaaatagcg caacatcgcc aattaataag cgggattcga tcaaattggt ggcgacggtt
    22741 aaatccgata acccttccag catgcactct tcaagcgtgc gcacactatg accgacttcc
    22801 agctttacat cccagagcag cgttaacagc tcgcccactt tttgcgcctg atcgtccggg
    22861 agctttttac ggcttaaaat cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga
    22921 ccgtagccac cgacggcgac caatgccagg tcggcaatct ggctgaatcc cgcttcaatc
    22981 cataatcgtt gcaggagctg gtcgataaac tcggtgcgcg cctcaatcaa ctgttctgca
    23041 gagatcccat tgtcaaaggc atcacccagc caacgctgga aagtatcgat atgggctttt
    23101 atcccaccga cggttaattc atcacggggc cagacgcatg gattttgcgg ttgaccgggc
    23161 agggtgggga gagcggtgtt tgcgtactgt tctggaaggg gattcattgt gcgccaccca
    23221 taagattaaa ttatcgcatt aaaaaagccg gcattcgccg gcttcatttt attcgtcgtg
    23281 cgagattatc gccgggatgg tgtcatcctt gcgtagcgtc agaatttcgc agccgttatc
    23341 agtcaccaca atagtatgct catattgtgc agacaagctg cgatctttgg tttttaccgt
    23401 ccagccatct ttcatggtgc ggatctcttt tttacccgcg ttgaccattg gctcgatggt
    23461 gaacgtcatc ccaggtttca gtacgacgtt ggtttcacgg gagtcatagt gcagcacctg
    23521 cggttcttca tggaagccgc gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa
    23581 gccttctgct tcgacaaatt tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg
    23641 ttttaccatg cgtagcgcta ggtacaggct ttcttgcgtg atacggcaca gacgttcgcc
    23701 catgatggtc ggcttaccga cgataaacat tttcgaggta tcgccgtgga aaccatcttt
    23761 gattacggtg acatcaatgt taacgatatc gccatctttc agcagcttag catcgtccgg
    23821 aataccgtgg cacaccactt cattaataga aatgcaaacg gatttcggat aaccgtgata
    23881 gccgaggcag gctgaaaccg cgtgttgttc attaacaatg taatcattac agatgcgatc
    23941 cagctcgccg gtgctgacgc ccggtttaac atatggttcg atcatctcca gcacttcggc
    24001 agccagtcgg ccagcgacgc gcattttttc gatatcttct ggggtcttga ttgagatagc
    24061 cattaattct gtccatcagc gtcggtgata ccgacaatat atatgtaagt gccgtcaatg
    24121 gtatcacacc cgggcaaatt gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt
    24181 tttctataat agaaaattcg acgtctgatg ctgtacacag cgccaacaat tattggtgtc
    24241 cacggcgtat ttgtggtata aagcgcgccg gacttccgat ccatttcgta tacacagact
    24301 ggacggaagc gacaatctca ctttgtgtaa caacacacac gtatcggcac atattccggg
    24361 gtgccctttg gggtcggtaa tatgggatac gtggaggcat aaccccaact tttatataga
    24421 ggttttaatc atggcaactg tttccatgcg cgacatgctc aaggctggtg ttcacttcgg
    24481 tcaccagacc cgttactgga acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa
    24541 agttcacatc atcaaccttg agaaaactgt accgatgttc aacgaagctc tggctgaact
    24601 gaacaagatt gcttctcgca aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag
    24661 cgaagcggtg aaagacgctg ctctgagctg cgaccagttc ttcgtgaacc atcgctggct
    24721 gggcggtatg ctgactaact ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct
    24781 ggaaactcag tctcaggacg gtactttcga caagctgacc aagaaagaag cgctgatgcg
    24841 cactcgtgag ctggagaaac tggaaaacag cctgggcggt atcaaagaca tgggcggtct
    24901 gccggacgct ctgtttgtaa tcgatgctga ccacgaacac attgctatca aagaagcaaa
    24961 caacctgggt attccggtat ttgctatcgt tgataccaac tctgatccgg acggtgttga
    25021 cttcgttatc ccgggtaacg acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt
    25081 tgctgcaacc gtacgtgaag gccgttctca ggatctggct tcccaggcgg aagaaagctt
    25141 cgtagaagct gagtaataag gcttgataac tcccccaaaa tagttcgagt tgcagaaagg
    25201 cggcaagctc gagaattccc gggagcttac atcagtaagt gaccgggatg agcgagcgaa
    25261 gatgacgcat ctgcggcgcg aaatatgaag ggggagagcc cttatagacc aggtagtaca
    25321 cgtttggtta gggggcctgc atatggcccc ctttttcact tttatatctg tgcggtttaa
    25381 tgccgggcag atcacatctc cgaggatttt agaatggctg aaattaccgc atccctggta
    25441 aaagagctgc gtgagcgtac tggcgcaggc atgatggatt gcaaaaaagc actgactgaa
    25501 gctaacggcg acatcgagct ggcaatcgaa aacatgcgta agtccggtgc tattaaagca
    25561 gcgaaaaaag caggcaacgt tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac
    25621 tacggcatca ttctggaagt taactgccag actgacttcg ttgcaaaaga cgctggtttc
    25681 caggcgttcg cagacaaagt tctggacgca gctgttgctg gcaaaatcac tgacgttgaa
    25741 gttctgaaag cacagttcga agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac
    25801 atcaacattc gccgcgttgc tgcgctggaa ggcgacgttc tgggttctta tcagcatggt
    25861 gcgcgtatcg gcgttctggt tgctgctaaa ggcgctgacg aagagctggt taaacacatc
    25921 gctatgcacg ttgctgcaag caagccagaa ttcatcaaac cggaagacgt atccgctgaa
    25981 gtggtagaaa aagaatacca ggtacagctg gatatcgcga tgcagtctgg taagccgaaa
    26041 gaaatcgcag agaaaatggt tgaaggccgc atgaagaaat tcaccggcga agtttctctg
    26101 accggtcagc cgttcgttat ggaaccaagc aaaactgttg gtcagctgct gaaagagcat
    26161 aacgctgaag tgactggctt catccgcttc gaagtgggtg aaggcatcga gaaagttgag
    26221 actgactttg cagcagaagt tgctgcgatg tccaagcagt cttaattatc aaaaaggagc
    26281 cgcctgaggg cggcttcttt ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct
    26341 gcgctgaaaa gcgacgtaca atgtcgctag tattaattca tttcaatcgt tgacagtctc
    26401 aggaaagaaa catggctacc aatgcaaaac ccgtctataa acgcattctg cttaagttga
    26461 gtggcgaagc tctgcagggc actgaaggct tcggtattga tgcaagcata ctggatcgta
    26521 tggctcagga aatcaaagaa ctggttgaac tgggtattca ggttggtgtg gtgattggtg
    26581 ggggtaacct gttccgtggc gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg
    26641 accacatggg gatgctggcg accgtaatga acggcctggc aatgcgtgat gcactgcacc
    26701 gcgcctatgt gaacgctcgt ctgatgtccg ctattccatt gaatggcgtg tgcgacagct
    26761 acagctgggc agaagctatc agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg
    26821 gtacaggtaa cccgttcttt accaccgact cagcagcttg cctgcgtggt atcgaaattg
    26881 aagccgatgt ggtgctgaaa gcaaccaaag ttgacggcgt gtttaccgct gatccggcga
    26941 aagatccaac cgcaaccatg tacgagcaac tgacttacag cgaagtgctg gaaaaagagc
    27001 tgaaagtcat ggacctggcg gccttcacgc tggctcgtga ccataaatta ccgattcgtg
    27061 ttttcaacat gaacaaaccg ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga
    27121 ctttaatcac ggaataattc ccgtgatgga taaataaggg taagattccg cgtaagtatc
    27181 gcgggggatc ttacccgtaa tatgtttaat cagggctata cttagcacac ttccactgtg
    27241 tgtgactgtc tggtctgact gagacaagtt ttcaaggatt cgtaacgtga ttagcgatat
    27301 cagaaaagat gctgaagtac gcatggacaa atgcgtagaa gcgttcaaaa cccaaatcag
    27361 caaaatacgc acgggtcgtg cttctcccag cctgctggat ggcattgtcg tggaatatta
    27421 cggcacgccg acgccgctgc gtcagctggc aagcgtaacg gtagaagatt cccgtactct
    27481 gaaaatcaac gtgtttgatc gttcaatgtc tccggccgtt gaaaaagcga ttatggcgtc
    27541 cgatcttggc ctgaacccga actctgcggg tagcgacatc cgtgttccgc tgccgccgct
    27601 gacggaagaa cgtcgtaaag atctgaccaa aatcgttcgt ggtgaagcag aacaagcgcg
    27661 tgttgcagta cgtaacgtgc gtcgtgacgc gaacgacaaa gtgaaagcac tgttgaaaga
    27721 taaagagatc agcgaagacg acgatcgccg ttctcaggac gatgtacaga aactgactga
    27781 tgctgcaatc aagaaaattg aagcggcgct ggcagacaaa gaagcagaac tgatgcagtt
    27841 ctgatttctt gaacgacaaa aacgccgctc agtagatcct tgcggatcgg ctggcggcgt
    27901 tttgcttttt attctgtctc aactctggat gtttcatgaa gcaactcacc attctgggct
    27961 cgaccggctc gattggttgc agcacgctgg acgtggtgcg ccataatccc gaacacttcc
    28021 gcgtagttgc gctggtggca ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat
    28081 tctctccccg ctatgccgta atggacgatg aagcgagtgc gaaacttctt aaaacgatgc
    28141 tacagcaaca gggtagccgc accgaagtct taagtgggca acaagccgct tgcgatatgg
    28201 cagcgcttga ggatgttgat caggtgatgg cagccattgt tggcgctgct gggctgttac
    28261 ctacgcttgc tgcgatccgc gcgggtaaaa ccattttgct ggccaataaa gaatcactgg
    28321 ttacctgcgg acgtctgttt atggacgccg taaagcagag caaagcgcaa ttgttaccgg
    28381 tcgatagcga acataacgcc atttttcaga gtttaccgca acctatccag cataatctgg
    28441 gatacgctga ccttgagcaa aatggcgtgg tgtccatttt acttaccggg tctggtggcc
    28501 ctttccgtga gacgccattg cgcgatttgg caacaatgac gcccgatcaa gcctgccgtc
    28561 atccgaactg gtcgatggga cgtaaaattt ctgtcgattc ggctaccatg atgaacaaag
    28621 gtctggaata cattgaagcg cgttggctgt ttaacgccag cgccagccag atggaagtgc
    28681 tgattcaccc gcagtcagtg attcactcaa tggtgcgcta tcaggacggc agtgttctgg
    28741 cgcagctggg ggaaccggat atgcgtacgc caattgccca caccatggca tggccgaatc
    28801 gcgtgaactc tggcgtgaag ccgctcgatt tttgcaaact aagtgcgttg acatttgccg
    28861 caccggatta tgatcgttat ccatgcctga aactggcgat ggaggcgttc gaacaaggcc
    28921 aggcagcgac gacagcattg aatgccgcaa acgaaatcac cgttgctgct tttcttgcgc
    28981 aacaaatccg ctttacggat atcgctgcgt tgaatttatc cgtactggaa aaaatggata
    29041 tgcgcgaacc acaatgtgtg gacgatgtgt tatctgttga tgcgagcgcg cgtgaagtcg
    29101 ccagaaaaga ggtgatgcgt ctcgcaagct gaggataatc cggctacaga gagtcgcgct
    29161 atttgttagc gtagggcttc agtgatatag tctgcgccat ctgatcgtaa gtagttggct
    29221 ttataaggtc aggtatgccg tggttttaca cggctttttt ttgtataggc ttcagtattc
    29281 ctgagtaccg taaaccctgt cagggaataa aaaacgcgtg atgttgtctg ctactcaacc
    29341 acttagcgaa aaattgccag cgcatggctg ccgtcatgtt gcgatcatta tggacggcaa
    29401 tggtcgctgg gcaaaaaagc aagggaagat tcgtgccttt gggcataaag ccggagcaaa
    29461 atccgtccgc cgggctgtct cttttgcggc caacaacggt attgaggcgt taacgctgta
    29521 tgcctttagt agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt taatggaact
    29581 gtttgtgtgg gcgctcgata gcgaagtaaa aagtctgcac cgacataacg tgcgtctgcg
    29641 tattattggc gataccagtc gctttaactc gcgtttgcaa gaacgtattc gtaaatctga
    29701 agcgctaaca gccgggaata ccggtctgac gctgaatatt gcggcgaact acggtggacg
    29761 ttgggatata gtccagggag tcaggcaact ggctgaaaag gtgcagcaag gaaacctgca
    29821 accagatcag atagatgaag agatgctaaa ccagcatgtc tgtatgcatg aactggcccc
    29881 tgtagattta gtaattagga ctggggggga gcatcgcatt agtaactttt tgctttggca
    29941 aattgcctat gccgaacttt actttacaga tgttctctgg cccgatttcg atgaacaaga
    30001 ctttgaaggg gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg gcaccgagcc
    30061 cggtgatgaa acagcctgat gggggtcgct tttgctgaag tatcgcctga tatctgcttt
    30121 tgtgttaata cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat
    30181 tgtaacgctg gtggtctgca tgctggcagc gtgggaatgg ggacagctta gcggttttac
    30241 cactcgttcg cagcgagtat ggttggcggt gttatgcggg ttattgttgg cgctgatgct
    30301 ttttctgttg ccggaatatc accgaaatat tcatcaaccg ctggttgaaa tctcactttg
    30361 ggcttcgctg ggttggtgga ttgtcgcgct attgctggtg ctgttttacc caggttccgc
    30421 agcaatctgg cgtaactcta aaacattgcg ccttattttt ggcgtgctaa ccattgttcc
    30481 cttcttctgg ggcatgctgg cgttacgggc ctggcactat gacgagaatc attacagtgg
    30541 cgcaatatgg ctgctctatg tcatgatcct ggtatggggc gctgactccg gcgcatatat
    30601 gtttggcaaa ttgtttggta aacataagct ggcaccgaag gtttctccgg gtaaaacctg
    30661 gcaaggcttt atcggtggac tcgctactgc agcggtaatc tcatggggtt atggcatgtg
    30721 ggcgaatctc gacgtcgctc ccgtcacctt actcatttgc tctattgtcg cagcgttagc
    30781 ctcagtgctc ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa ttaaggacag
    30841 cggtcattta attccaggac acggtggtat tttagatcgt attgatagcc tgacggctgc
    30901 ggtaccggtc tttgcttgct tgttgttact ggtattcagg acgctttaac ggaaggtaat
    30961 atgctgagtt ttctctggga tttggcttcg ttcatcgttg cactgggtgt acttatcacc
    31021 gtgcatgaat ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt tgagcgtttc
    31081 tcaatagggt ttggtaaggc gctctggcgg cgaactgata agctcggcac cgaatatgtt
    31141 atcgccctga tcccgttggg cggttatgtc aaaatgctgg atgagcgcgc agaaccggtc
    31201 gttccggaac tccgccacca tgccttcaat aataaatctg tcggccaacg agcggcgatt
    31261 attgccgcag gtccggttgc aaacttcatt tttgctatct ttgcctactg gctggttttt
    31321 attattggtg tgcctggcgt acgtccggtg gttggcgaaa tagcagccaa ttcgatagct
    31381 gcggaagcac aaattgcacc aggtacggaa ctaaaagccg tagatggtat cgaaacgcct
    31441 gattgggatg ccgtgcgttt gcagttggtc gataaaattg gcgatgaaag caccaccatt
    31501 acagtagcgc catttggcag cgaccaacgg cgggatgtaa agctcgattt acgtcactgg
    31561 gcgtttgagc ctgataaaga agatccggta tcttcgctgg ggattcgtcc tcgtgggccg
    31621 caaattgaac ctgtactgga aaatgtgcag ccaaactcgg cggcaagcaa ggcaggtttg
    31681 caagcaggcg acaggatcgt taaagtcgat ggtcagccct taacgcagtg ggtgaccttt
    31741 gtgatgcttg tccgggataa cccgggtaaa tccttagcgt tagaaatcga aaggcagggg
    31801 agtcccttgt ctttgacatt aatcccggag agtaaaccgg gtaatggtaa agcgattggt
    31861 tttgtcggta ttgagccgaa agtcattcct ttgccagatg agtataaagt tgtacgccag
    31921 tatgggccgt tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct gatgaagctg
    31981 acggtcagta tgctgggaaa attgatcacc ggtgatgtga aactgaacaa cctcagtggg
    32041 ccgatctcta tcgccaaggg ggctgggatg acagcggaac tcggggtagt ttattacctg
    32101 ccgtttcttg cgcttattag cgtgaactta gggataatta acctgtttcc gttgcccgta
    32161 cttgacgggg ggcatctgct gttccttgcg atcgaaaaga tcaaaggcgg accggtatcc
    32221 gagcgggttc aagacttttg ttatcgcatt ggctcgattc tgctggtgct gttaatgggg
    32281 cttgcacttt tcaatgattt ctctcggtta tgagagttag ttaggaagaa cgcataataa
    32341 cgatggcgat gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc accgtatacg
    32401 gtgctgaagg gttcgtagtg aaagatattc atttcgaagg ccttcagcgt gtcgccgttg
    32461 gtgcggccct cctcagtatg ccggtgcgca caggcgacac ggttaatgat gaagatatca
    32521 gtaataccat tcgcgctctg tttgctaccg gcaactttga ggatgttcgc gtccttcgtg
    32581 atggtgatac ccttctggtt caggtaaaag aacgtccgac cattgccagc attactttct
    32641 ccggtaacaa atcggtgaaa gatgacatgc tgaagcaaaa cctcgaggct tctggtgtgc
    32701 gtgtgggcga atccctcgat cgcaccacca ttgccgatat cgagaaaggt ctggaagact
    32761 tctactacag cgtcggtaaa tatagcgcca gcgttaaagc tgtcgtgacc ccgctgccgc
    32821 gcaaccgtgt tgacctaaaa ctggtgttcc aggaaggtgt gtcagctgaa atccagcaaa
    32881 ttaacattgt tggtaaccat gctttcacca ccgacgaact gatctctcat ttccaactgc
    32941 gtgacgaagt gccgtggtgg aacgtggtag gcgatcgtaa ataccagaaa cagaaactgg
    33001 cgggcgacct tgaaaccctg cgcagctact atctggaccg cggttatgcc cgtttcaaca
    33061 tagactctac ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc acggtgaaca
    33121 tcaccgaagg cgatcagtac aagctttctg gcgttgaagt gagcggcaac cttgccgggc
    33181 actccgctga aattgagcag ctgactaaga tcgagccggg cgagctgtat aacggcacca
    33241 aagtgaccaa aatggaagat gacattaaaa agcttctcgg tcgctatggt tatgcctatc
    33301 cgcgcgtaca gtcgatgcct gaaattaacg atgccgacaa aaccgttaaa ttacgcgtga
    33361 acgttgatgc gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt aacgatacct
    33421 cgaaagatgc cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg ctgggaagcg
    33481 atctggtcga tcagggtaag gagcgtctga atcgtctggg cttctttgaa actgtcgata
    33541 ccgataccca acgtgttccg ggtagcccgg accaggtaga tgtagtctac aaggtgaaag
    33601 aacgtaacac tggtagcttc aacttcggga ttggttacgg tactgaaagt ggcgtgagct
    33661 tccaggctgg cgtgcaacag gataactggt taggtacagg ttatgctgtt ggtatcaacg
    33721 ggaccaaaaa cgattaccag acctatgctg aactgtcggt aaccaacccg tacttcaccg
    33781 tagatggcgt aagcctcggt ggtcgtctct tctataatga cttccaggca gatgacgccg
    33841 acctgtccga ctataccaac aagagttatg gtacagacgt gacgttgggc ttcccgatta
    33901 acgaatataa ctcgctgcgt gcaggtctgg gttatgtaca taactccctg tccaacatgc
    33961 agcctcaggt tgcgatgtgg cgttatctgt actctatggg tgaacatccg agcacctctg
    34021 atcaggataa cagcttcaaa acggacgact tcacgttcaa ctatggttgg acctataaca
    34081 agcttgaccg tggttacttc ccgacagatg gttcacgtgt caacctgacc ggtaaagtga
    34141 ccattcctgg atcggataac gaatactaca aagtgacgtt agacacggcg acttatgtgc
    34201 cgatcgatga cgatcacaaa tgggttgttc tggggcgtac ccgctggggt tatggtgatg
    34261 gtttaggcgg caaagagatg ccgttctacg agaacttcta tgccggtggt tccagcaccg
    34321 tgcgtggctt ccagtccaat acaattggtc cgaaagcagt ttacttcccg catcaggcca
    34381 gtaattatga tccggactat gattacgaat gtgcgactca ggacggcgcg aaagacctgt
    34441 gtaaatcgga tgatgctgta ggcggtaacg ccatggcggt tgccagcctc gagttcatca
    34501 ccccgacgcc gtttattagc gataagtatg ctaactcggt tcgtacttcc ttcttctggg
    34561 atatgggtac cgtttgggat acaaactggg attccagcca atattctgga tatccggact
    34621 atagtgatcc aagcaatatc cgtatgtctg cgggtatcgc attacaatgg atgtccccat
    34681 tggggccgtt ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga gacaaggcag
    34741 aacagttcca gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag gaatgtagtg
    34801 gtagtgtagc gatgacttta ggcgatcaat ataaatcgcc gggccacgca aagaactgca
    34861 ccctccggtg caaatgggat ggtaaggagt ttattgtgaa aaagtggtta ttagctgcag
    34921 gtctcggttt agcactggca acatctgctc aggcggctga caaaattgca atcgtcaaca
    34981 tgggcagcct gttccagcag gtagcgcaga aaaccggtgt ttctaacacg ctggaaaatg
    35041 agttcaaagg ccgtgccagc gaactgcagc gtatggaaac cgatctgcag gctaaaatga
    35101 aaaagctgca gtccatgaaa gcgggcagcg atcgcactaa gctggaaaaa gacgtgatgg
    35161 ctcagcgcca gacttttgct cagaaagcgc aggcttttga gcaggatcgc gcacgtcgtt
    35221 ccaatgaaga acgcggcaaa ctggttactc gtatccagac tgctgtgaaa tccgttgcca
    35281 acagccagga tatcgatctg gttgttgatg caaacgccgt tgcttacaac agcagcgatg
    35341 taaaagacat cactgccgac gtactgaaac aggttaaata agtaatgcct tcaattcgac
    35401 tggctgattt agcgcagcag ttggatgcag aactacacgg tgatggcgat atcgtcatca
    35461 ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat tacgttcatg gttaacccaa
    35521 aataccgtga gcatttaggc ttgtgccagg cgtccgcggt tgtcatgacc caggacgatc
    35581 ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc ctacctgact tacgcgcgca
    35641 tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa cattgcaccc agtgcggtga
    35701 tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg cgctaacgcg gtgattgaat
    35761 ccggcgttga actgggcgat aacgtgatta tcggtgccgg ttgcttcgta ggtaaaaaca
    35821 gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac catttaccat gagatccaga
    35881 tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg cgcagacggc tttggttatg
    35941 ccaacgatcg tggtaactgg gtgaagatcc cacagattgg tcgcgtaatt attggcgatc
    36001 gcgtggagat cggtgcctgc actaccatcg atcgcggcgc gctggatgac actgttattg
    36061 gcaatggcgt gatcattgat aaccagtgcc agattgcaca taacgtcgtg attggcgaca
    36121 atacggcggt tgccggtggc gtcattatgg cgggcagcct gaaaattggt cgttactgca
    36181 tgatcggcgg agccagcgta atcaacgggc atatggaaat atgcgacaaa gtgacggtta
    36241 cgggcatggg tatggtgatg cgtcccatca ctgaaccagg cgtctattcc tcaggcattc
    36301 cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact ggtgatgaac attgatgaca
    36361 tgagcaagcg tctgaaatcg cttgagcgca aggttaatca acaagactaa cgttccatct
    36421 tttgttcgcc aaactttacg gcctgtctca ttcttacgat tgcggcaggc cgtgttatta
    36481 ttgtcgtttc ttatattttg acaggaagag tatcttgact actaacactc atactctgca
    36541 gattgaagag attttagaac ttctgccgca ccgtttcccg ttcttactgg tggatcgcgt
    36601 gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa aatgtctctg tcaatgagcc
    36661 attcttccag ggccatttcc ctggaaaacc gattttcccg ggtgtgctga ttctggaagc
    36721 aatggcacag gcaacaggta ttctggcgtt taaaagcgta ggaaaactgg aaccgggtga
    36781 gctgtactac ttcgctggta ttgacgaagc gcgcttcaag cgcccggtcg tgcctggcga
    36841 tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc ggcctgaccc gttttaaagg
    36901 ggttgctctg gtcgacggta aagtagtttg cgaagcaacg atgatgtgtg ctcgtagccg
    36961 ggaggcctga tacgtgattg ataaatccgc ctttgtgcat ccaaccgcca ttgtggaaga
    37021 gggcgcgtcg attggcgcga acgcacacat tggtcctttt tgtatcgttg gaccccatgt
    37081 cgaaattggt gagggtaccg tactgaaatc tcacgttgtc gtgaatggtc atactaaaat
    37141 tggccgcgat aatgagattt atcagttcgc ctccatcggc gaagttaacc aggatctgaa
    37201 atatgctggc gaaccgaccc gcgtggaaat cggcgatcgt aaccgcattc gcgaaagcgt
    37261 caccattcat cgtggcacag tccagggcgg tggattgacg aaggtgggca gcgacaactt
    37321 actgatgatc aacgcgcaca ttgcgcacga ttgtacggta ggtaaccgct gtattctcgc
    37381 caacaacgca acgctggcgg gtcacgtatc ggttgacgac ttcgcgatca tcggcggcat
    37441 ggccgcagtc catcagttct gcatcattgg tgcgcatgtg atggttggcg gctgctccgg
    37501 tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt aaccacgcca cgccgttcgg
    37561 tgtcaatatc gaagggctga agcgccgcgg attcagccgt gaggcgatta ccgctatccg
    37621 caatgcgtat aagctgattt atcgtagcgg taaaacgctc gatgaagtga aaccggaaat
    37681 tgctgaactg gcggaaacat atccggaagt gaaagccttt accgatttct ttgcacgctc
    37741 aacgcgcggt ctgattcgtt aatgactgaa cagcgtccat taacgattgc cctggtcgcc
    37801 ggagaaacct ccggcgatat cctgggggcc ggtttaatcc gcgctctgaa agaacgtgtg
    37861 cccaacgccc gctttgttgg tgttgccggg ccacgaatgc aggctgaagg ctgcgaagct
    37921 tggtacgaaa tggaagagtt ggcggtgatg ggcattgttg aagtgctcgg tcgtctgcgt
    37981 cgcttactgc atattcgtgc cgatctgaca aagcgttttg gtgaactaaa gccagatgtt
    38041 tttgttggca ttgatgcgcc tgacttcaat attatcctcg aaggtaacct taaaaagcag
    38101 ggtatcaaaa ccattcatta tgtcagtccg tccgtctggg cgtggcgaca gaaacgcgtt
    38161 ttcaaaatag gcagagccac cgatctggtg ctcgcatttc tgcctttcga aaaagcgttt
    38221 tatgacaaat acaacgtacc gtgccgcttt atcggtcata ccatggctga tgccatgcca
    38281 ttagatccag ataaaaatag tgcccgtgat gtgctgggga tcccttacga tgcccactgt
    38341 ctggcattgt tgccgggcag ccgtggtgca gaagtcgaaa tgcttagtgc cgatttcctg
    38401 aaaacggccc agcttttgcg ccagacatat ccggatctcg aaatcgtggt gccactggtg
    38461 aatgccaaac gccgcgagca gtttgaacgc atcaaagctg cagtcgcgcc agacctgtcg
    38521 gttcatttgc tggatggaat gggccgtgag gcgatggtcg ccagcgatgc ggcactactg
    38581 gcgtcgggga cggcagccct ggagtgtatg ctggcgaaat gcccgatggt ggtgggatat
    38641 cgcatgaagc cttttacctt ctggttggcg aagcggctgg tgaaaactga ttatgtctcg
    38701 ctgccaaatc tgctggcggg cagagagtta gtcaaagagt tattgcagga agagtgtgag
    38761 ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga atgggaaaac cagccacgcg
    38821 atgcacgata ccttccgtga actgcatcag cagatccgct gcaatgccga tgagcaggcg
    38881 gcacaagccg ttctggagtt agcacaatga tcgaatttgt ctatccgcac acgcagctgg
    38941 ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg cgcggtcgtc accgctgcgg
    39001 tgattctcga cccggcgcgc ccgattgccg ggctgaatga ttccaaaaag ctgagcgaaa
    39061 aacgtcgtct ggcgctctgt gaagagatca aagagaaagc gttgagctgg agtctgggcc
    39121 gcgcggaacc tcacgaaatc gacgaactga atattttaca tgcgaccatg ctggcgatgc
    39181 agcgagccgt cgctgggctg catattgcgc cggaatatgt gttgattgat ggtaaccgct
    39241 gcccgaaatt accgatgcct gcgatggctg tggtgaaagg cgatagccgc gtaccggaaa
    39301 tcagtgccgc gtctatcctg gcgaaagtga cgcgtgacgc cgaaatggcg gcgctggata
    39361 ttgttttccc gcaatatggt tttgcccaac acaaagggta tccaaccgct tttcatctgg
    39421 aaaaactggc tgaacacggc gcgaccgaac accatcggcg cagctttggg cctgtcaaac
    39481 gcgcactggg acttgcgtcc tgattcttgt gtcgagatta agtaaaccgg aatctgaaga
    39541 tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg atcgatggcc
    39601 tggccaaaac cgcaccgctg gtaaaaaagg cggcggcgtt gggtatgcca gcactggcga
    39661 tcaccgattt caccaacctt tgcggtctgg tgaagttcta cggagcggga catggcgcag
    39721 ggattaagcc tatcgtcggg gcagatttta acgtccagtg cgacctgctg ggtgatgagt
    39781 taacccacct gacggtactg gcggcgaaca ataccggcta tcagaatctg acgttgctga
    39841 tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc gatcatcgat cgcgactggc
    39901 ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg gcgcatgggc gacgtcggac
    39961 gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg tgtcgcgttt tatgaagaac
    40021 acttcccgga tcgctatttt ctcgagctga tccgcaccgg caggccggat gaagaaagct
    40081 atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt gcccgtcgtg gcgactaacg
    40141 atgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc gcgatccacg
    40201 acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag caatatatgc
    40261 gtagcgaaga ggagatgtgc gagctgtttg ccgacatccc agaagccctt gccaacaccg
    40321 ttgagatcgc caaacgctgt aacgtaactg tgcgtcttgg tgaatacttc ctgccgcagt
    40381 tcccgaccgg ggacatgagc accgaagatt atctggtcaa gcgtgcaaaa gagggcctgg
    40441 aagagcgtct ggccttttta ttccctgatg aggaagaacg tgttaagcgc cgcccggaat
    40501 atgacgaacg tctggagact gaacttcagg ttatcaacca gatgggcttc ccgggctact
    40561 tcctcatcgt tatggaattt atccagtggt cgaaagataa cggcgtaccg gtagggccag
    40621 gccgtggctc cggtgcgggt tcactggtgg cctacgcgct gaaaatcacc gacctcgatc
    40681 cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc tccatgcctg
    40741 acttcgacgt tgacttctgt atggagaaac gcgatcaggt tatcgaacac gtagcggaca
    40801 tgtacggtcg tgatgcggta tcgcagatta tcaccttcgg tacgatggcg gcgaaagcgg
    40861 tgatccgcga cgtaggccgc gtgctggggc atccgtacgg ctttgtcgat cgtatctcga
    40921 aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc gagccgcagc
    40981 tgccggaaat ctacgaagcg gatgaagaag ttaaggcgtt gatcgacatg gcgcgcaaac
    41041 tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc gcgccgacca
    41101 aaattaccga ttttgcgccg ctttattgcg atgaagaggg caaacatccg gtcacccagt
    41161 ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt ggtttgcgta
    41221 cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg aagaatggcg
    41281 agccgccgct ggatatcgcc gcgatcccgc tggatgacaa gaaaagcttc gacatgctgc
    41341 aacgctcgga aaccacggcg gtattccagc ttgaatcgcg cggcatgaag gacctgatca
    41401 agcgtctgca acctgactgc ttcgaagata tgatcgcact ggtggcactg ttccgccctg
    41461 gtccgttgca atcagggatg gtggataact ttatcgaccg taaacatggt cgcgaagaga
    41521 tctcctatcc ggacgtgcag tggcagcatg aaagcctgaa accggtactg gagccaacct
    41581 acggcattat cctgtatcag gaacaggtca tgcagattgc gcaggtgctt tctggttata
    41641 ccctcggtgg cgcggatatg ctgcgtcgtg cgatgggtaa gaaaaagccg gaagagatgg
    41701 ctaagcaacg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac gccgaactgg
    41761 cgatgaaaat cttcgacctg gtggagaaat tcgctggtta cggatttaac aaatcgcact
    41821 ctgcggccta tgctttggtg tcatatcaaa cattatggtt gaaagcgcac tatccggcag
    41881 agtttatggc ggcggtaatg accgccgata tggacaacac cgagaaggtg gtgggcctgg
    41941 tggatgagtg ctggcggatg gggctgaaaa tcctgccacc agatataaac tccggtcttt
    42001 accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg tattggcgcg atcaaagggg
    42061 taggtgaagg tccgattgag gcgatcatcg aagcccgtaa taaaggcggc tacttccgcg
    42121 aactgtttga tctctgcgcc cgaaccgaca ccaaaaagtt gaaccgtcgc gtgctggaaa
    42181 aactgatcat gtccggggcg tttgaccgtc ttgggccaca tcgcgcggcg ctgatgaact
    42241 cgctgggcga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct atcggtcagg
    42301 ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat tgaacaatcc tatgccagct
    42361 gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta ggcctgtacc
    42421 tgaccggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc ggaggcgtaa
    42481 ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacggctgcg gggctcgttg
    42541 ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc acgctggatg
    42601 accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa taccagcatt
    42661 tgctggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat gacttcagcg
    42721 gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg gaaaaatatg
    42781 ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt ttaaaccgac
    42841 tccgtcagtc tctggaaccc caccgctctg ggacaattcc agtacatctc tactatcaga
    42901 gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct ccgagcgatc
    42961 gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg gagtttgact
    43021 aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt gcagagctgg
    43081 aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg gatattaaca
    43141 tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacgcgt aaaatcttcg
    43201 ccgatctcgg tgcatggcag attgcgcaac tggcacgcca tccacagcgt ccttataccc
    43261 tggattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac cgcgcgtatg
    43321 cagacgataa agctatcgtc ggtggtatcg cccgtctcga tggtcgtccg gtgatgatca
    43381 ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt ggtatgccag
    43441 cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc tttaagatgc
    43501 ctatcatcac ctttatcgac accccggggg cttatccggg cgtgggcgca gaagaacgtg
    43561 gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgcctcggc gtaccggtag
    43621 tttgtacggt tatcggtgaa ggtggttctg gcggtgcgct ggcgattggc gtgggcgata
    43681 aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt tgtgcgtcca
    43741 ttctgtggaa gagcgccgac aaagcgccgc tggcggctga agcgatgggt atcatcgctc
    43801 cgcgtctgaa agagctgaaa ctgatcgact ccatcatccc ggaaccgctg ggcggcgctc
    43861 accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgctggcg gatctggccg
    43921 atctcgacgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc ctgatgagct
    43981 acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc ccttttttat
    44041 cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac acatgaacat
    44101 cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca aagaactgga
    44161 gtcggcgctg gtggcgcaag gctttcagat tatctggcca caaaacagcg ttgatttgct
    44221 gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg atgaatacag
    44281 tctcgattta tgtagcgata tcaatcagct taatgaatat ctcccgcttt atgccttcat
    44341 caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc tctgtttttt
    44401 tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt acaccgacga
    44461 atatcttgat aacattaccc caccgttcac gaaagccttg tttacctacg tcaaagagcg
    44521 gaagtacacc ttttgtacgc cgggtcatat gggcggcacc gcatatcaaa aaagcccggt
    44581 tggctgtctg ttttatgact ttttcggcgg gaataccctt aaggctgatg tctctatttc
    44641 ggtcaccgag cttggttcgt tgctcgacca caccgggcca cacctggaag cggaagagta
    44701 catcgcgcgg actttcggcg cggaacagag ttatatcgtt accaacggaa catcgacgtc
    44761 gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt acgctgttga ttgaccgcaa
    44821 ttgtcataaa tcgctggcgc atctgttgat gatgaacgat gtagtgccag tctggctgaa
    44881 accgacgcgt aatgcgttgg ggattctggg tgggatcccg cgccgtgaat ttactcgcga
    44941 cagcatcgaa gagaaagtcg ctgccaccac gcaggcacaa tggccggttc atgcggtgat
    45001 taccaactca acctatgatg gcttgctcta caacaccgac tggatcaaac agacgctgga
    45061 tgtcccgtcg atccacttcg attccgcctg ggtgccgtac acccattttc atccgatcta
    45121 ccaggggaaa agtggtatga gcggcgagcg tgttgcggga aaagtgatct tcgaaacgca
    45181 atcgacccac aaaatgctgg cggcgttatc gcaggcttcg ctgatccaca ttaaaggtga
    45241 gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct cgcccagtta
    45301 tcccattgtt gcttcggttg agacggcagc agcgatgctg cgtggtaatc cgggcaaacg
    45361 gctgattaac cgttcagtag aacgagctct gcattttcgc aaagaggtcc agcggctgcg
    45421 ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg atgaagccga
    45481 atgctggccc gttgcgcctg gcgaacaatg gcacggcttt aacgatgcgg atgccgatca
    45541 tatgttcctc gatccggtta aagtcactat tctgacgccg gggatggacg agcagggcaa
    45601 tatgagcgag gaggggatcc cggcggcgct ggtggcgaag ttcctcgatg agcgtgggat
    45661 cgtggtagag aaaaccggcc catataacct gctgtttctc tttagtattg gcatcgataa
    45721 aaccaaagca atgggattat tgcgtgggtt gacggaattt aaacgctctt acgatctcaa
    45781 tctgcgaatc aaaaatatgc ttccggatct gtacgcagaa gatcccgatt tctaccgcaa
    45841 tatgcgtatt caggatctgg cgcaagggat ccataagctg attcgtaaac acgatcttcc
    45901 cggtttgatg ttgcgggcat tcgatacttt gccggagatg atcatgacgc cacatcaggc
    45961 atggcagaga cagattaaag gtgaagtaga aaccattgcg ctggaacaac tggtcggtag
    46021 agtatcggca aatatgatcc tgccttatcc accgggcgta ccgctgttga tgcctggaga
    46081 aatgctgacc aaagagagcc gcacagtact cgattttcta ctgatgcttt gttccgtagg
    46141 gcaacattac cccggttttg aaacggatat tcacggtgcg aaacaggacg aagacggcgt
    46201 ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt ccgggcgagt
    46261 aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt tcaccatatt
    46321 gcgattattg cgacggatta tgcggtgagc aaagctttct actgcgatat tcttggtttc
    46381 acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga tttggcgctt
    46441 aatgggcaat atgtgattga gcttttctca tttccgttcc cgccggagcg ccccagccga
    46501 ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgacatcga tgcggcagtg
    46561 gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc atacacgcaa
    46621 aaacgcttca ccttctttaa cgatccggac gggctgccgt tggaactgta tgagcagtaa
    46681 ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt aaaatcgcac
    46741 catcatgaca ctcacgctca atagacaact tctcacctca cgccagattc tggtggcctt
    46801 tagcggcggg cttgactcca ccgttctgct gcatcagttg gtgcagtggc ggacggaaaa
    46861 tccggggggc gctctgcgcg ctattcatgt gcatcacggt ttaagtgcca atgccgatgc
    46921 ctgggttacg cattgcgaaa acgtctgcca acagtggcag gtgccgctgg tggtcgaacg
    46981 cgtacaactt gcgcaagagg ggctggggat tgaggcccag gcgcggcagg cgcgttatca
    47041 ggcatttgcc cgcaccttgt tgcccggtga agtgctggtc accgcgcaac atctcgacga
    47101 tcaatgtgaa acttttctgc tggcgctaaa acgcggtagc ggccctgccg ggctttcggc
    47161 tatggcggaa gtttcggagt ttgccggaac acggcttatc cgcccgttgc ttgcccgcac
    47221 gcggggggaa ctggcgcagt gggcattggc gcatggttta cgctggattg aagacgaaag
    47281 taatcaggac gactcatacg atcgtaactt tctgcgcctg cgcgtagtgc cgttattgca
    47341 gcagcgttgg ccgcattttg ccgaaacaac ggcccgcagc gccgcacttt gtgctgaaca
    47401 agagagcctg ctggatgaac tgctggcaga tgatttagca cactgtcaat cgccgcaggg
    47461 gacgctgcag attgtgccaa tgctggcgat gagtgatgcc cgccgcgcgg cgattatccg
    47521 ccgctggctg gcagggcaga atgcaccgat gccttcccgc gacgcgttgg tgaggatctg
    47581 gcaggaagtg gcgctggcgc gggaagatgc ctccccctgt ttacgtttgg gcgcgtttga
    47641 aatccgacgc tatcagtcgc aactgtggtg gattaaatcc gtcaccgggc aaagcgaaaa
    47701 cattgtgccg tggcagacat ggcttcaacc gctggaatta ccggcggggc agggaagtgt
    47761 acagcttaat gcgggaggcg atattcgccc tccgcgtgca gacgaagcgg tcagcgtgcg
    47821 tttcaaagcg ccaggattgc tgcatattgt cgggcgtaac ggcggacgta agctaaagaa
    47881 aatctggcaa gagctgggcg tgccgccgtg gctacgtgac accacgccac tgctgtttta
    47941 tggcgaaacg ctgattgcgg cggcaggggg atttgtgacg caagaaggtg tggctgaagg
    48001 tgagaatggc ataagttttg tctggcagcg aaacgcttag tttagtgaag gccggatatg
    48061 acgcattaag cgtcgcatcc ggcgaaagtc aatcaggact cgctcaccac caccgtaccg
    48121 atttccgggt ggctaaagct ggtaatttta tccagacgta gctcacgggt ttcgccagcg
    48181 gcctcgacga ccagatactc cacatttttg cgggagacta aatcgctggc tttggcctgc
    48241 aatttttcgc catctttcag ctcaagtgtc agcattaaat gatgctggca ggcgagctca
    48301 agattatcgt aatcatcaca attgattggt tgatacgtat cattcattga cataatcgct
    48361 caccagtaag tttgccgcag cgtatgctgc tttttccctg acagcctcag aaagggcgtc
    48421 gtcggcagcc atttcattca gcactttcaa aacgcagccc agcgcgtccg gaacgtatcc
    48481 taagtctccg ctggcgattt ccgcgtaccg cttgcgtatt aactcacaat atttttccac
    48541 atgccctcct gtcagcactc tgacttaacc gtggatgcaa gtctaagcct acgaagataa
    48601 actctgtttc gcaaggtgac tataccacac tcatttctgc aatatcagcg ccgcaactgc
    48661 acgtattccg ttacaatggc ctcctgattc gaaaggagtt ttcttatggc gcttaaagcg
    48721 acaatttata aagcgacggt taatgtggcc gatctcgacc gcaaccagtt tctcgatgcc
    48781 tctctgacgc tggcgcgcca cccttcagaa acccaggagc gtatgatgct gcgcctgctg
    48841 gcgtggctga aatatgctga tgaacgtctg caatttaccc gtggtttgtg tgccgatgat
    48901 gagccggaag cgtggctgcg taacgatcac ctgggcattg atttgtggat tgagctgggg
    48961 ctgccggatg agcgacgaat taagaaagcc tgcacccagg ccgcagaagt ggcgctgttt
    49021 acctataata gtcgggcggc gcaaatctgg tggcagcaaa atcagagcaa atgtgcgcag
    49081 tttgccaatc tttccgtctg gtatctggac gacgagcagc tggcgaaagt gagcgccttt
    49141 gccgatcgta ccatgacgct gcaggcaacg attcaggatg gcgtgatctg gttatcggat
    49201 gataagaata atctggaagt gaacttaacc gcctggcaac aaccttcatg attgtgattt
    49261 cccgacatgt tgctattccc gatggtgagc ttgagatcac cgccattcgt gcgcagggcg
    49321 cgggcgggca gcatgttaat aagacctcaa cggccattca tctgcgtttt gacattcggg
    49381 catccagcct gccagagtat tacaaagagc gtctgctcgc cgccagccat catttgatca
    49441 gcagtgatgg cgtaattgtc attaaggcac aggaataccg cagtcaggaa ctgaaccgcg
    49501 aagcggcgct ggcccggctg gtggcagtga ttaaagattt aacaacagaa caaaaagccc
    49561 gacgacccac gcggcccacc cgtgcatcga aagagcgcag gctggcatcg aaagcacaaa
    49621 aatcaagcgt gaaggcgatg cgcagcggtc gggaataaaa agaaggaatg gatggtgaaa
    49681 aaagcgatag tgacagcgat ggctgtaatc agcctcttta ctctgatggg ttgtaataat
    49741 cgggccgaag tcgatacgct ttctccggcg caggctgccg aattgaaacc gatgccgcaa
    49801 agttggcgcg gcgtgctgcc gtgtgccgat tgcgaaggaa tcgaaacctc tctgttcctc
    49861 gaaaaagacg gaacatgggt gatgaatgag cgttatctcg gtgctcgtga agaaccttcc
    49921 tccttcgctt cctacggtac atgggcgcga accgctgaca agctggtatt aaccgacagc
    49981 aaaggtgaaa agtcatatta tcgggcgaaa ggcgatgcgc tggagatgct cgatcgtgaa
    50041 ggcaatccga ttgaatcgca gttcaactat acgctggaac cggcacaatc cagtttaccc
    50101 atgacgccga tgaccctgcg gggcatgtat ttttatatgg ctgatgcggc gaccttcact
    50161 gattgcgcga ccggaaaacg tttcatggta gcgaataacg cagagctgga gcgtggctac
    50221 ctggctgcgc gcggccacag tgaaaaaccg gtgttactgt cagtagaagg tcactttacg
    50281 cttgaggcta atccggatac cggtgcgcca accaaagtat tagcacccga tacggcaggg
    50341 aaattttacc caaaccagga ttgcagtagt ttggggcagt aacccgtctt gagacagaaa
    50401 caaacgcaaa acaggccaga aggatatatt tcaacatttt gaatttgcac gttttttgta
    50461 ggccggataa ggcgtttacg ccgcatccgg caatggtgct caacgcctga tgcgacgctg
    50521 gcgcgtctta tcatgcctac aacccccctc ataccttaag gcggataagg catttacttt
    50581 atcaccgggt caaaccaaca aagcgacccg ccttgatata aatccctggc ttcaggtgcc
    50641 caacatactg taactgcgtt tctggcttaa acgcagagac atcaccaata cgcatatgca
    50701 tcagatcggc agggctaatc cagcccgatt gcgccagcgt aagcggatgc ccggctttgg
    50761 caaatgcatc agtgacaaat tccgaacaaa accacgactt tttgtctcct tcgaccacac
    50821 tgcttaactg cgctttcgcc aggccgctga cgcactgttg gcgaaaatcc tcggagaacg
    50881 gattcagtga gcacatctgg cgagtcacca taaagggaat aaattcgaca atgccgcgat
    50941 agttataacc gctatctttg attttattgg cgaacgcggt gatttctgtg gcttgttgcg
    51001 gggtaagatc cgggactcgt aagacgaaaa gtttatcact atgcttcatc gcttttttaa
    51061 gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac gttattttca cccagataga
    51121 ttgcaacgtg actcacagag gaagtgctga aaacgcggat cccaaatgag gttacctcca
    51181 ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg taccgttatt tcttttattg
    51241 attgttcggt gaaagagctt tgatgctgga atttgacagc ccatgttttc gcctcggcat
    51301 ctactgctgt ggctgatgag tctggctggc tgatatcaac ggtacaggcc gaaagtaaaa
    51361 gaaagcaggg gagaagcagg cggcagtacg cctttggttt atccatttta tacaatccat
    51421 gtaaaaaagg gccctgaaat tcaggaccct ttctggcatc agcctttaat ctgtttcacc
    51481 agatattcga cgatgtcacc agtcttaatt aactgtttct cgccgttgcg acgatattta
    51541 tattcgatat cgtcgttgtc gaggttacgg tcgcccagca caatagtgtg cggaataccg
    51601 atcagttcca tatcggcaaa catcacgccc ggacgttctt ttcggtcatc cagcagcact
    51661 tcgatacctt gtgcacgcag ttcgctgtac agtttctcag caagctcttg tacgcggaag
    51721 gatttgtgca tgttcatcgg cagaattgcc acctggaacg gcgcgatagc gtcaggccat
    51781 acgatgccgc gctcgtcgta gttctgctca atcgccgcag ccactacgcg cgttaccccg
    51841 ataccgtagc aacccatagt caggatttgg ttacggccat cttcaccctg tacggaggct
    51901 ttcagtgctt cggagtactt ggtacccagc tggaagatgt gaccaacttc gataccacgt
    51961 ttgatcagca gcgtaccctg gccatccggg cttggatcgc cagccaccac gttacggata
    52021 tccgcaactt ccggggtagc gacatcgcga tcccagttga tgccgaagta gtgtttacca
    52081 tcgatgttag caccagcagt gaaatcactc atcgccgcaa cggtacggtc aatcaccacc
    52141 ggaatcggca tgtttaccgg acccagtgaa cccggaccgg ctttaaccac ggcacgaatt
    52201 tcttcttcgg tcgcgaaagt cagcgggctg gcaacctgcg gcagtttttc tgctttaact
    52261 tcgttcagct cgtgatcacc gcgcaccagc agcgcaacca gcgggaagct actgccttca
    52321 accgctttaa ccagcagagt cttaaccgtt ttcttaatcg gcagattgaa ctgttcaacc
    52381 agttccgcga tggttttcgc gttcggcgta tcaaccagcg tcatttcctg ggtagcagca
    52441 gcgcgcggtt ctttcggcgc gatagcttct gccagctcaa tgttcgctgc atagtcagag
    52501 gtgtcggaga agaccacatc gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag
    52561 gcgctgccgc cgatagaacc ggtgtcggcc tgtacggcgc ggaaatccag ccccatgcgg
    52621 ctgaagattt tgctgtaggc cgcatacatt gcatcgtagg tttcctgcag ggattcctga
    52681 gaagtatgga aagagtaagc atctttcatc aggaattcgc gggaacgcat gacgccgaaa
    52741 cgcggacgca cttcgtcgcg gaacttggtc tggatctgat agaagttcag cggcagctgt
    52801 ttgtaagagc taagctcgtt acgaatcagg tcagtgataa cttcttcatg agttgggccg
    52861 agtacgaacg gacgctcgcc acggtcaaca aaacgcagca gttccggacc gtactgttcc
    52921 caacgaccac tctcttgcca caaatcggct ggctgaacca ccggcatcga cacctcgatt
    52981 gcaccggcgt tgttcatctc ttcacgcacg atgttttcga cttttttcag aacgcgcacg
    53041 ccggtcggca gccaggtata taacccggag gccagcttgc ggatcatccc ggcgcgcagc
    53101 atcagctgat ggctgatcac ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg
    53161 tattggctag tacgcatgtt gttacggttc cagttggaag gtagaacagg ctcaaggcga
    53221 gcctgggaca aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg
    53281 cgtgaattta acgcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc
    53341 gaacgttaaa atcatgtagc cagacggcat aggttttgcc cgtttcctca cctttacgat
    53401 aggccgggcg cgggtcctgc gccagtactt cgcggataaa cagcgttaac tgcggataac
    53461 gcttctccag cgtcaaaagc tgcttttcga cctctgcggt aaaactcacc gccatctctg
    53521 cagctggcgc actttgcgca tagctggcac tggcatcggg aagcgattcg gcaaagggga
    53581 gatacggttt gatatccact accggcgtac catcgaccag atccaggctg ccgagcttca
    53641 gaatcacgca gtctttatgg caaacaacct ctttcagctc taccagcgac ataccaatcg
    53701 ggttggggcg gaaagtggag cgcgtagcga aaacacccat tctggcatta ccgccaagac
    53761 gaggcggacg cacagtcgga cgccagccgc cttccatcgt ttgatgaaag acgaaaagga
    53821 tccataaatg gctgaatgct tccaggccgc gaacggcgtc agcctggttg tagggagcaa
    53881 tgagatgtag ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga acggcgaatt
    53941 tttctttata gggcgagcga ataacaccta tttgctcgaa ctggaaactg ctcatttcgc
    54001 cgtaatgtta agcgcagaac cgatacataa agcctgacga tagcagcctg gcgtaccgct
    54061 ggtaacttcg cagctatgca gtaataccgc attggctttc attttcgagg cgttgatttg
    54121 catccgctta cgcgcggttg gaatgctcgg cggagagtcc tgattagagg cctggcaaga
    54181 gtcgccactg acttcaccga gatcgcggaa cggtttgccg actaattctt ctgcattggt
    54241 ataaattcgg accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg
    54301 gggtgcagtg ctttgaacgg gttcgacagg ggatctgctt aacatggaac agccgcttag
    54361 catgagtgct actaaacaga tcggtaaagc acgcatagta tttcctcaat gtatgatcaa
    54421 aacgtcaata ttgaatcagg agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga
    54481 atgatattac aaattgtgga aacagcctaa aaattaccag cctttaacag cgccgccgtt
    54541 aaacactttg tttgctgctt cgtaaacttc gtcagactga taagcctgga cgaatttctt
    54601 cacgttctcg gcgtctttgt tatcttcacg cgttacgatc aggtttacgt acggggagtc
    54661 tttatcttcg acaaagatac cgtttttcgc tggagtcagg ccaatctggc tggcataggt
    54721 ggtgttaata actgccagag cgatttgcgc gtcgtccaga gaacgcggca gttgcggtgc
    54781 ttccagttca acaattttca gattttttgg gttctcaaca acatcaagaa cggtcggcag
    54841 caggccaacg ccatctttca gtttgatcaa gcccactttt tgcagcagca gcagtgaacg
    54901 accaaggtta gttggatcgt ttggcacggc aacctgcgaa ccatcctgca gttcatccag
    54961 tgatttgatt ttcttggagt aaccagcaat cggataaaca aaagtgttgc ctactgcgac
    55021 cagtttgtag ccacgatctt tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt
    55081 ggcgtcgata tcgcctttgc tcaatgcttc gtttggcaga acatagtcgt tgaaggttac
    55141 cagctcaacg tccaggccat atttgtcttt cgcaactttc tgcgcgactt ctgcaacctg
    55201 ctgttcggca ccaacaatca cgccgacttt aatgtggttt ggatcttttt catcctgacc
    55261 gcagcctacc agtgccagtg atccaatcag ggctcccact gccgcaaagg ttttgaattt
    55321 gaacgccata ccttattcct tcttcaatta tttatgttgt gttgaacgtt acttgcgagt
    55381 gacagcccgg acgatgcggt cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa
    55441 taccagtacc gtattcatca ccgtcgcgtt atagccgatg tagccatact gatagccaat
    55501 ctgacctaaa ccaccggcac cgactgcacc acccatcgcg gaataaccga ccagggtaat
    55561 cagggtgata gttgccgcat tcaccagacc cggcaacgct tccggtaaca gcactttacg
    55621 gacgatctgc attggcgtgg cacccattgc gcgggaagct tcaattaacc cggttgggat
    55681 ctccagcaga gcgttctcga ccatacgggc aataaacggt gctgcaccaa cggttaacgg
    55741 aacaattgct gcctgcaaac caatcgatgt accgacaata acgcgggtaa acggaatcat
    55801 ccataccagc aagataatga acgggatgga acggaaaatg ttcacaatcg cagaaacggt
    55861 acgatacagc ttcgcgttag caataatttg ccccggacgc gtgacataaa gcaggacgcc
    55921 aaccggcaga ccaaccacaa acccaaaaaa tccggatacg aaggtcattg ccagcgtttc
    55981 ccatacgcca cgaaccagca gccacatcat cggctcagac ataacccagt acctctactt
    56041 ttacatggtg ttcctgcagc caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca
    56101 tttcagtcag catgatgccg aacttaacgc caccggcgta atccatctgc gcgctaataa
    56161 tgttgttgtt gacgttgaaa cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt
    56221 gaccggtaaa ctccagacgc agcatcggca cgcagtcagt aaatggctcc gcttgcagac
    56281 gttcctggta atcttccggg atatccagat gcagggttga ctgaataaac ttctgcgcca
    56341 gcggcgtttt cggatgtgag aacacttcac ttaccgtgtc ctgctcgatc agttcgccat
    56401 tgctgatgac cgcaacgcaa tcacaaatac gcttcacaac gtccatttcg tgggtgatca
    56461 acagaatcgt caaacccaga cggcggttaa tgtctttcag cagttcgaga atagaacgtg
    56521 tcgttgccgg gtctagcgcg ctggtggctt catcacacag caatactttg ggattgctgg
    56581 ctaatgcacg ggcaatcgcc acacgttgtt tctgcccacc ggaaagattc gacgggtagc
    56641 tatcatgctt atctccaaga ccaaccaatg acagcaattc cgtcacgcga cgtttgatct
    56701 cgtctttcgg tgtattgtcc agctccagcg gcagagccac gttgccaaaa acagtacgcg
    56761 aagagagcag gttaaaatgc tggaaaatca taccaatctg gcggcgagct ttggtcaact
    56821 cggattctga cagcgtggtc agttcctgtc catcgaccag cacgctaccc tcggttgggc
    56881 gctccagcag gtttacacaa cgtataagcg tactcttacc cgcgcctgag gcaccgataa
    56941 cgccataaat ttgtccagct ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc
    57001 gggtgccctg gtggaacact ttggtgatat tcgaaagttt tatcattgat tatttattat
    57061 cgtcattaag ttagtcgtgg catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat
    57121 ggatgttaag gcatccagac gtctaaatca atcaggttta tgcgaagagc actttcttgc
    57181 aggtcgaaac atgcgatact agcgtcacat gccttattaa ggagctataa aaggtggcga
    57241 agagcgtacc cgcaattttt cttgaccgtg atggcaccat taatgtcgat cacggctatg
    57301 tccatgagat cgacaacttt gaatttatcg acggtgttat tgacgccatg cgcgagctaa
    57361 aaaaaatggg ctttgcgctg gtggtagtca ccaaccagtc tggcattgct cgcggtaaat
    57421 ttaccgaagc acagtttgaa acgctgaccg agtggatgga ctggtcgctg gcggaccgag
    57481 atgtcgatct ggatggtatc tattattgcc cgcatcatcc gcagggtagt gttgaagagt
    57541 ttcgccaggt ctgcgattgc cgcaaaccac atccggggat gcttttgtca gcacgcgatt
    57601 atttgcatat tgatatggcc gcttcttata tggtgggcga taaattagaa gatatgcagg
    57661 cagcggttgc ggcgaacgtg ggaacaaaag tgctggtgcg tacgggtaaa cctattacac
    57721 ctgaagcaga aaacgcggca gattgggtgt taaatagcct ggcagacctg ccgcaagcga
    57781 taaaaaagca gcaaaaaccg gcgcaatgat taaaagatga gcggttgaaa taaaaatgca
    57841 tttttccgct tgtcttcctg agccgactcc ctataatgcg cctccatcga cacggcgaat
    57901 gtgaatcact tcacacaaac agccggttcg gttgaagaga aaaaat
//
LOCUS       DAZFBR010000016        57118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFBR010000016 DAZFBR010000000
VERSION     DAZFBR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.016"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            2..496
                     /gene="lpfA1"
                     /locus_tag="ACHJZ2_001152"
     CDS             2..496
                     /gene="lpfA1"
                     /locus_tag="ACHJZ2_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFO4567310.1"
                     /translation="MALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVDSQNQEVVLGQ
                     VQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDLISVSTEAGAA
                     KGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTTGYGNAQVDFN
                     LSYE"
     gene            561..1247
                     /gene="lpfB"
                     /locus_tag="ACHJZ2_001153"
     CDS             561..1247
                     /gene="lpfB"
                     /locus_tag="ACHJZ2_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO4567311.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGALNITLPAGVGSVSKIKYNTINDFGS
                     SAEMITKNVD"
     gene            1276..3849
                     /gene="lpfC"
                     /locus_tag="ACHJZ2_001154"
                     /pseudo
     CDS             1276..3849
                     /gene="lpfC"
                     /locus_tag="ACHJZ2_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
     gene            3864..4919
                     /locus_tag="ACHJZ2_001155"
     CDS             3864..4919
                     /locus_tag="ACHJZ2_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4567312.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            4924..5454
                     /gene="lpfE"
                     /locus_tag="ACHJZ2_001156"
     CDS             4924..5454
                     /gene="lpfE"
                     /locus_tag="ACHJZ2_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO4567313.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5709..7400
                     /gene="eptB"
                     /locus_tag="ACHJZ2_001157"
     CDS             5709..7400
                     /gene="eptB"
                     /locus_tag="ACHJZ2_001157"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO4567314.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7492..7568
                     /locus_tag="ACHJZ2_001158"
     tRNA            7492..7568
                     /locus_tag="ACHJZ2_001158"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7526..7528,aa:Pro,seq:cgg)
     gene            8313..9920
                     /gene="dppA"
                     /locus_tag="ACHJZ2_001159"
     CDS             8313..9920
                     /gene="dppA"
                     /locus_tag="ACHJZ2_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO4567315.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10071..11090
                     /gene="dppB"
                     /locus_tag="ACHJZ2_001160"
     CDS             10071..11090
                     /gene="dppB"
                     /locus_tag="ACHJZ2_001160"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO4567316.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11100..12002
                     /gene="dppC"
                     /locus_tag="ACHJZ2_001161"
     CDS             11100..12002
                     /gene="dppC"
                     /locus_tag="ACHJZ2_001161"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO4567317.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12013..12996
                     /gene="dppD"
                     /locus_tag="ACHJZ2_001162"
     CDS             12013..12996
                     /gene="dppD"
                     /locus_tag="ACHJZ2_001162"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO4567318.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            12993..13997
                     /gene="dppF"
                     /locus_tag="ACHJZ2_001163"
     CDS             12993..13997
                     /gene="dppF"
                     /locus_tag="ACHJZ2_001163"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO4567319.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14027..15298)
                     /gene="yhjV"
                     /locus_tag="ACHJZ2_001164"
     CDS             complement(14027..15298)
                     /gene="yhjV"
                     /locus_tag="ACHJZ2_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO4567320.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            15774..15881
                     /gene="ldrD"
                     /locus_tag="ACHJZ2_001165"
     CDS             15774..15881
                     /gene="ldrD"
                     /locus_tag="ACHJZ2_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4567321.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(15968..17647)
                     /gene="bcsG"
                     /locus_tag="ACHJZ2_001166"
     CDS             complement(15968..17647)
                     /gene="bcsG"
                     /locus_tag="ACHJZ2_001166"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO4567322.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGLESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTSGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17644..17835)
                     /gene="bcsF"
                     /locus_tag="ACHJZ2_001167"
     CDS             complement(17644..17835)
                     /gene="bcsF"
                     /locus_tag="ACHJZ2_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO4567323.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(17832..19403)
                     /gene="bcsE"
                     /locus_tag="ACHJZ2_001168"
     CDS             complement(17832..19403)
                     /gene="bcsE"
                     /locus_tag="ACHJZ2_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO4567324.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            19676..19864
                     /gene="bcsR"
                     /locus_tag="ACHJZ2_001169"
     CDS             19676..19864
                     /gene="bcsR"
                     /locus_tag="ACHJZ2_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO4567325.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPGIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            19876..20628
                     /gene="bcsQ"
                     /locus_tag="ACHJZ2_001170"
                     /pseudo
     CDS             19876..20628
                     /gene="bcsQ"
                     /locus_tag="ACHJZ2_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            20625..23243
                     /gene="bcsA"
                     /locus_tag="ACHJZ2_001171"
     CDS             20625..23243
                     /gene="bcsA"
                     /locus_tag="ACHJZ2_001171"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO4567326.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            23254..25593
                     /gene="bcsB"
                     /locus_tag="ACHJZ2_001172"
     CDS             23254..25593
                     /gene="bcsB"
                     /locus_tag="ACHJZ2_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO4567327.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            25600..26706
                     /gene="bcsZ"
                     /locus_tag="ACHJZ2_001173"
     CDS             25600..26706
                     /gene="bcsZ"
                     /locus_tag="ACHJZ2_001173"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO4567328.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            26688..30161
                     /gene="bcsC"
                     /locus_tag="ACHJZ2_001174"
     CDS             26688..30161
                     /gene="bcsC"
                     /locus_tag="ACHJZ2_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO4567329.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWALQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLRDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            30243..32231
                     /gene="hmsP"
                     /locus_tag="ACHJZ2_001175"
     CDS             30243..32231
                     /gene="hmsP"
                     /locus_tag="ACHJZ2_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO4567330.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            32414..33700
                     /gene="dctA"
                     /locus_tag="ACHJZ2_001176"
     CDS             32414..33700
                     /gene="dctA"
                     /locus_tag="ACHJZ2_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO4567331.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            33921..35417
                     /gene="yhjJ"
                     /locus_tag="ACHJZ2_001177"
     CDS             33921..35417
                     /gene="yhjJ"
                     /locus_tag="ACHJZ2_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO4567332.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(35513..36442)
                     /gene="kdgK"
                     /locus_tag="ACHJZ2_001178"
     CDS             complement(35513..36442)
                     /gene="kdgK"
                     /locus_tag="ACHJZ2_001178"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO4567333.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            36674..37441
                     /gene="pdeH"
                     /locus_tag="ACHJZ2_001179"
     CDS             36674..37441
                     /gene="pdeH"
                     /locus_tag="ACHJZ2_001179"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO4567334.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            37511..39571
                     /gene="yhjG"
                     /locus_tag="ACHJZ2_001180"
     CDS             37511..39571
                     /gene="yhjG"
                     /locus_tag="ACHJZ2_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4567335.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(39753..41075)
                     /gene="yhjE"
                     /locus_tag="ACHJZ2_001181"
     CDS             complement(39753..41075)
                     /gene="yhjE"
                     /locus_tag="ACHJZ2_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4567336.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(41487..42500)
                     /gene="yhjD"
                     /locus_tag="ACHJZ2_001182"
     CDS             complement(41487..42500)
                     /gene="yhjD"
                     /locus_tag="ACHJZ2_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO4567337.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(42549..43448)
                     /gene="rcdB"
                     /locus_tag="ACHJZ2_001183"
     CDS             complement(42549..43448)
                     /gene="rcdB"
                     /locus_tag="ACHJZ2_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4567338.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            43968..44570
                     /gene="yhjB"
                     /locus_tag="ACHJZ2_001184"
     CDS             43968..44570
                     /gene="yhjB"
                     /locus_tag="ACHJZ2_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO4567339.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(44621..46270)
                     /gene="treF"
                     /locus_tag="ACHJZ2_001185"
     CDS             complement(44621..46270)
                     /gene="treF"
                     /locus_tag="ACHJZ2_001185"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO4567340.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            46675..48072
                     /gene="ccp"
                     /locus_tag="ACHJZ2_001186"
     CDS             46675..48072
                     /gene="ccp"
                     /locus_tag="ACHJZ2_001186"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO4567341.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            48283..49683
                     /gene="gadA"
                     /locus_tag="ACHJZ2_001187"
     CDS             48283..49683
                     /gene="gadA"
                     /locus_tag="ACHJZ2_001187"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO4567342.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            50054..50878
                     /gene="gadX"
                     /locus_tag="ACHJZ2_001188"
     CDS             50054..50878
                     /gene="gadX"
                     /locus_tag="ACHJZ2_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO4567343.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
     gene            51246..51974
                     /gene="gadW"
                     /locus_tag="ACHJZ2_001189"
     CDS             51246..51974
                     /gene="gadW"
                     /locus_tag="ACHJZ2_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO4567344.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(52337..55450)
                     /gene="mdtF"
                     /locus_tag="ACHJZ2_001190"
     CDS             complement(52337..55450)
                     /gene="mdtF"
                     /locus_tag="ACHJZ2_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO4567345.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(55475..56584)
                     /gene="mdtE"
                     /locus_tag="ACHJZ2_001191"
     CDS             complement(55475..56584)
                     /gene="mdtE"
                     /locus_tag="ACHJZ2_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO4567346.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            56692..56970
                     /locus_tag="ACHJZ2_001192"
     CDS             56692..56970
                     /locus_tag="ACHJZ2_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567347.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(56971..>57118)
                     /gene="gadE"
                     /locus_tag="ACHJZ2_001193"
     CDS             complement(56971..>57118)
                     /gene="gadE"
                     /locus_tag="ACHJZ2_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO4567348.1"
                     /translation="LKNKNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGI
                     TSYF"
BASE COUNT        13882 a        14120 c        15287 g        13829 t
ORIGIN      
        1 actggcgtta acttctggca ctgtattcgc agcagagtct ggtgacggca cggttaaatt
       61 tacaggcgag atcgtggatt caccttgcgt actgtccgtt gactctcaga accaggaagt
      121 tgtgttgggt caggtacaaa aatctgtatt tgctgcggtt ggtgacaaat ccccggcaaa
      181 acctttcgaa atcaaactgg aagattgcga taccaccacg atgaaaaaag ccaatgtgag
      241 ctttagtggt gttggtgatg cagataaatc tgatctgatt tctgttagca ctgaagcggg
      301 cgcggccaaa ggtgttggta tcggtattta tgataacagc aataccctgg tcgctcttaa
      361 cggtggtaaa gcaagtgttg atctgagtaa aggccagacg gttctctact ttaccgctaa
      421 ctacgtttct actctggcaa cggtaacgac cggttacggc aatgcgcagg tcgatttcaa
      481 cctgtcttac gagtaatcgt ccgttttaca aatggcggcg taattgccgc cattagtcgt
      541 caaagttcat ggatcgaatg atgaaaagta aatttgttgc gttggctctc tctctgttcc
      601 tttcacagtc agttctggcg ggtggcgtcg gacttagctc tactcgcgta atttatgatg
      661 gcagtaagaa agaagcctcg ctgaccgtac agaataaaag taagacggaa gagtttctta
      721 tccaatcctg ggtggatgat gccgcgggca gcaaaaaaac accgtttatt attacgcccc
      781 cactattcaa gcttgatcca gaaaagaaca atattttgcg tattgtgaat attacgcccg
      841 gcctgcctca ggatcgcgaa tcggtctact gggttaatgt gaaagccatt ccatctaaaa
      901 gtgacgacag cgaaaacaaa aatgttttgc agattgccgt tcgcacccga atcaaacttt
      961 tttatcgccc ggcaggttta aagggtgatg taaaaaccgc gccaaatgaa ctgcgattta
     1021 cgcgtaacgg taatcagctc cgcgttgata atccgacagt ttttaatatt acctttaatc
     1081 aattttttgc gaatgacaaa gaaattgaaa aagcaggcat ggtacccgct aaaggggcat
     1141 taaatatcac attacccgcc ggggtaggta gcgtttctaa aattaaatat aacaccatca
     1201 atgactttgg atcgtcagcg gaaatgatca ctaaaaatgt tgattgatta atatcttttc
     1261 aggagaaata gctcaatgtc acggaagacc gtttcacgta ctttttcttc cttttctatc
     1321 tctgtggtgg cggttgctgt tgccagcacg ttttctgcac atgccggaaa gtttaacccc
     1381 aaattcctgg aggacgttca gggtgtgggc cagcatgtcg atttaacgat gttcgaaaaa
     1441 ggccaggaac agcagttacc cggcatctat cgtgtctctg tttacgtcaa tgaacagaga
     1501 atggaaaccc gcacactgga atttaaagag gccacagaag cacagcgcaa agcgatgggg
     1561 gaatcccttg tgccttgcct tagccggacg caactggcag agatgggcgt gcgcgttgaa
     1621 agctttccgg ccctcaatct ggtgtctgcc gaagcctgcg taccgtttga cgaaatcatt
     1681 cccctggcct caagccattt cgacttcagt gaacaaaagc tggtattaag tttccctcag
     1741 gcggcgatgc accaggtggc gcgcggcacg gtgccggaat ctttatggga cgaaggtatc
     1801 cctgcgttgc tgctggatta cagttttagc gggagcaaca gtgaatatga ctcgacgggg
     1861 tcttccagca gttatgtcga tgataatggc actgttcatc atgatgatgg taaagatacg
     1921 ctaaagagcg acagctacta cctcaactta cgcagcgggc tgaaccttgg cgcttggcgg
     1981 ttacggaatt acagtacctg gagccattcc ggtggcaaag cgcagtggga taatatcggt
     2041 acgtctttga gtagggccat catccctttc aaagcgcaat taacaatggg ggatacggcg
     2101 accgcggggg acatttttga cagcgttcag atgcgtggcg cgatgttagc ctctgatgaa
     2161 gagatgctgc ccgacagcca gcgaggcttt gcacccatcg ttcgagggat cgccaaaagc
     2221 aacgccgaag tcagtattga acaaaacggc tacgttattt atcgcaccta tgtgcagcct
     2281 ggtgcctttg aaataaacga tctttacccc actgcaaaca gcggtgatct cacggtcatt
     2341 attaaagaag cggatggcag tgaacaacgc tttatttagc cattctcatc ggtgccaatt
     2401 ttccagcgtg aaggccatct caaatatagc tttgccgcag gtgaatatca ggccgggaat
     2461 tatgacagcg cctcgccgcg tttcgggcag cttgatctga tctacggttt accgtggggg
     2521 atgacggcct acggcggcgt attaatctct aataattaca atgcatttac attagggata
     2581 gggaaaaact ttggttatat cggggcgatt tccattgatg tgacgcaggc taaaagcgaa
     2641 ctgaataacg atcgcgatag ccagggacaa tcttatcgtt tcttatattc caagagcttc
     2701 gaaagcggca ccgatttccg ccttgcgggc tatcggtact ctaccagcgg tttctatacc
     2761 ttccaggaag ccaccgatgt gcgcagtgac gctgacagcg actataaccg ttatcacaag
     2821 cgcagcgaaa tacagggtaa cctgacgcag caattagggg cctatggctc tgtttattta
     2881 aatttaacgc agcaggatta ctggaacgac gcaggtaaac agaacacggt atcggcgggt
     2941 tacaacggac gtattggcaa ggtcagttac agtattgcat atagctggaa taaaagccct
     3001 gaatgggatg aaagcgatcg cttgtggtct ttcaatattt ccgttccact aggccgggcc
     3061 tggagtaact atcgcgtcac gaccgaccag gatggtcgta ccaatcaaca ggttggggtc
     3121 agcggaacgc tgcttgagga tcgcaacctg agctacagtg tccaggaagg ctacgccagc
     3181 aacggtgtgg gtaacagcgg taacgctaac gttggctatc agggtgggtc cggtaatgtc
     3241 aacgtaggct atagctacgg gaaagattac cggcagctca actacagcgt tcgcggcggc
     3301 gtgatagttc atagcgaagg cgtgacgctt tcccaaccgc taggcgaaac catgacgctc
     3361 atctccgtac ccggtgcgcg caatgcccgc gtggtgaata acggcggcgt tcaggttgac
     3421 tggatgggta acgcgatcgt gccttatgcc atgccgtatc gtgaaaacga aatctcactg
     3481 cgtagcgatt cgttgggtga cgatgttgac gttgaaaatg cgttccagaa agtggtgcca
     3541 acgcgtggag cgattgtcag agcgcgtttt gatacccgcg ttggttaccg cgtattaatg
     3601 acgctgcttc gttccgcggg cagcccggtg ccctttggag caacggcaac gctaatcacc
     3661 gataaacaaa acgaggtgag cagtatcgtt ggtgaagaag gacagctcta tattagcgga
     3721 atgccagagg aaggacgggt attgattaaa tggggtaatg acgcgtcgca gcaatgcgtg
     3781 gcgccttata aattatccct ggaattaaaa cagggcggaa ttattcctgt ttcggccaat
     3841 tgccagtaag caaggagagc gaaatgaaag cggcaatagc attatcctta ttaggatgcg
     3901 tattcggatt ttctggcaag gcatttgccg gagatgcgtg ggggccatgt acccccgcgg
     3961 atggcacgac ttatcactat aatgtcgatg tggatgttgg tatacctgat gcggcaaaaa
     4021 acgtcgcagg aacggtttta ccggatgtcc ttaactggtc taatggacaa aacgtatcgc
     4081 taatttgtga atgtcctgat tcttataaga atgaaaaaga tactctagtg cagggggtga
     4141 gtatgcttcc cccttctggc cgtacggttg acagtatgaa atattatact ttgacggagg
     4201 aactggaggt tgcgacgaac attcgcatca gtaccagtgt gtatgggttt gttcctttca
     4261 aaaaccagca ggcgctgcaa acaaccggat gcaacaaagt cattactacg ccctatatgg
     4321 gcggcgcagg tctactttcc tttgctatta ctaaaccctt tatcggcgat tccgtcatcc
     4381 ctctgacgtt aattgccgaa ctgtatgcct cgaaaacaaa taaagattac ggaacaatac
     4441 ccatatcttc cgtatctatt caggggcgtg tcaccgtaac ccaggattgc gaaattaaac
     4501 ccggcacggt gctggatgtg ccatttggtg aattcccctc ctcggcgttt aaaaacaggc
     4561 aagggcaaat gcctgaaggt gcgacagagc aagaaattaa cctcagcttc gattgcaaca
     4621 atatctctga tggtatcaag gttgcgttgc gccttgaggg ggcaaccaat gctgacgatc
     4681 cccgagcggt ggatatgggc aaccccgata ttggcgtact ggtgaaagat tccagcggca
     4741 aaattttggt accgaatgat tcaagcagca ccacgttatt aaatttgagc agcctggatt
     4801 ctaaaactca ccgcaatgcc gcgattcgac tgctggcttt accgattagc acgacgggta
     4861 aagcgcctaa aggcgggacg tttgaaggcg taacgaccat atatcttgaa atggagtgat
     4921 gccatgaagt tcaaacgatt gctgcatagc ggcatcgcca gtttgagtct ggttgcctgc
     4981 ggggtgaatg cggcgacgga tcttggcccg gcaggggata ttcatttctc catcactatc
     5041 accactaaag cttgcgagat ggaaaaaagc gatctcgaag tcgatatggg aacaatgacg
     5101 ctgcaaaaac ctgcggcagt cggtacggtg ttgagcaaga aagatttcac cattgaactc
     5161 aaagagtgcg atgggatatc caaagcgacc gttgagatgg acagtcagtc ggacagcgat
     5221 gatgattcca tgtttgccct tgaggctggt ggcgcaacgg gtgttgcgtt gaagatagag
     5281 gacgataaag gaacgcagca agttcccaaa ggctccagcg gaacgccgat tgaatgggcg
     5341 attgatggcg aaaccacgtc gcttcactac caggcgagtt atgtggtcgt caacactcag
     5401 gccactggtg gcacagcgaa tgcccttgtc aatttttcca tcacctatga gtaagttact
     5461 gggccagcat cctggcccaa cctatctctg aatagtcgtt aatccgttaa aatggtctat
     5521 tccggacttg cacttttgta aatttgtgct tcatgcacac tctttcccca cactttttcc
     5581 ctttgctgtg gtctacttat tcgcgcgtgt agattttact tatctgacta cctccgcact
     5641 tttcccctgc tgggcctgaa aagccgctaa gcagggtgtt atcacctgtt tgtccagggt
     5701 ttgtttgcat gagatacatc aaatcgatta cacagcagaa gctgagcttt ttgcttgcaa
     5761 tctatattgg cctttttatg aatggcgcgg ttttttaccg ccgcttcggc agctatgcgc
     5821 acgattttac cgtctggaaa ggcatttctg ctgttgttga actggccgcc accgtactgg
     5881 tgaccttctt tttactacgt cttctttcgc tgtttggccg ccgcagctgg cgtattctgg
     5941 catcgctggt tgtgctcttt tccgcaggtg ccagctatta catgaccttc cttaatgtgg
     6001 tcattggtta tggcatcatc gcttccgtca tgaccaccga tatcgacctg tcaaaagaag
     6061 ttgttggtct gaactttatt ctctggttaa tcgccgttag cgcattgcct cttatcctta
     6121 tctggaataa ccgctgtcgc tacaccttgc tccgacaact gcgaacccca gggcagcgta
     6181 ttcgcagcct ggcggtcgtc gtactggcgg gaattatggt ttgggcaccg attcgtttgc
     6241 tggatatcca gcagaagaaa gtggagaggg cgaccggcgt tgatttgccg agttatggcg
     6301 gtgtcgtagc gaactcttat ctgccatcaa actggctttc tgcgttgggg ctgtacgcct
     6361 gggcgcgggt cgatgaatct tccgataata attcattgct taacccggcg aagaaattca
     6421 cttatcaggc accgcaaaat gttaatgata cttatgtcgt gtttatcatc ggtgaaacca
     6481 cgcgttggga ccatatgggc attttcggct atgagcgtaa taccacgccg aagctggccc
     6541 aggagaaaaa tctggcggcg ttccgtggtt actcctgtga taccgcaacc aaactctctc
     6601 tgcgttgcat gtttgtacgt caggggggcg cggaagataa tccgcagcgc acattaaaag
     6661 aacagaacat tttcgcggta ctgaagcagt taggattcag ttctgacctc tacgctatgc
     6721 agagcgaaat gtggttctac agcaatacga tggcggacaa tattgcttac cgtgaacaga
     6781 ttggtgcgga gccacgtaac cgtggcaagc cggtagatga tatgttgctg gtagacgaaa
     6841 tgcagcaatc gctggggcgc aacccggatg gtaagcatct aatcattctg cataccaaag
     6901 gttcgcactt taactacacc cagcgttacc cgcgcagctt tgcgcagtgg aagccggaat
     6961 gtattggtgt tgatagcgga tgtaccaaag cgcagatgat caactcctat gacaactcgg
     7021 tgacctatgt ggatcacttt atctccagtg tgattgatca ggttcgcgat aagaaagcga
     7081 ttgtgttcta cgcagctgac cacggcgagt caattaatga acgcgagcac ctgcacggca
     7141 cgccgcgtga actggcaccg ccggagcagt tccgcgtacc gatgatggtc tggatgtcag
     7201 ataaatatct ggaaaatccg gtcaatgcgc aggcgtttgc gcagctgaaa aaagcagccg
     7261 acatgaaagt gccacgccgt cacgtagagc tgtacgacac catcatgggt tgtcttggct
     7321 atacttcacc ggatggtgga attaacgaaa acaacaactg gtgtcacatc ccgcagacaa
     7381 aagaggcagc ggctaactaa gaggcctgct gactttctcg ccgatcaaaa ggcattttgc
     7441 tattaaggga ttgacgaggg cgtatctgcg cagtaagatg cgccccgcat tcggtgattg
     7501 gcgcagcctg gtagcgcact tcgttcggga cgaaggggtc ggaggttcga atcctctatc
     7561 accgaccaaa ttcgaaaagc ctgctcaacg agcaggcttt tttgcatctg cagcccatga
     7621 ggaagagaac ctccgggggc agggaggttc gactcgacgt aggcctgata agacgcgtca
     7681 gcgtcgcatc aggcaacggc accgcactta ttgtcggatg cggcgtgaac gccttattcg
     7741 acctacacgc cacacatttc cctgccaaat ttgcgcccca tatctcacta tccattaacg
     7801 gatttgtgac agaaaccatt gtcttttttt atatttgctt aaatcttttt ttgcgttact
     7861 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
     7921 atatccacct tttcctcaga tttttttcac taaatgcata aaacttaatc accaaatgtt
     7981 gctaaatatt aagctgattg ttctggtcat gttggagtag cacaaatttc tctgctgcaa
     8041 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
     8101 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
     8161 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
     8221 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
     8281 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
     8341 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
     8401 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
     8461 gtaccaccta tgacgcctct tccgtaccgc tttataaccg cctagttgaa tttaaaatcg
     8521 gcaccaccga agtgattccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
     8581 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
     8641 cgcgcgaaat gaatgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
     8701 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggcatg ggcttgccgg
     8761 agctgatcag cgaagtgaaa aaggtggacg acaacaccgt tcagtttgtg ctgactcgcc
     8821 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
     8881 atgctgatgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggca
     8941 ccggtccgtt ccagttacag cagtatcaaa aagattcccg tatccgctac aaagcgtttg
     9001 atggctactg gggcaccaaa ccgcagatcg atacgctggt tttctctatt acccctgacg
     9061 cttccgtgcg ttacgctaaa ttgcagaaaa atgaatgcca ggtgatgccg tacccgaacc
     9121 cggcagatat cgcccgcatg aagcaggata aatctatcaa cctgatggaa atgccggggc
     9181 tgaacgtcgg ttatctctcg tataacgtgc agaaaaaacc actcgatgac gtgaaagttc
     9241 gccaggctct gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
     9301 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
     9361 aggactacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa
     9421 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
     9481 gccgcatggc ggagatgatt caggcagact gggcgaaagt cggcgtgcag gccaaaattg
     9541 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgagcac cagacggtaa
     9601 tgatgggctg gactggcgat aacggggatc cggataactt cttcgccact ctgttcagct
     9661 gcgccgcctc tgaacaaggc tccaactact caaaatggtg ctacaaaccg tttgaagatc
     9721 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
     9781 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
     9841 aaccggtacg caaagaagtt aaaggctatg tggttgatcc attaggcaaa catcacttcg
     9901 aaaacgtctc tatcgaataa ttaaaagcca tacccggtgg tgctgtgcct ctgcgtgcag
     9961 cgccatccgg cagcaatact tcattccctg cccgacctgg gcgggaattg atttgtgagc
    10021 aatacagaca cgcagttcca ggctgcgggt cactacagag aatccgggtt atgttgcagt
    10081 ttattctccg acgtttggga ctcgtcatcc ccacgtttat cggtattacc cttctcacat
    10141 ttgcctttgt ccacatgatc ccgggcgatc ctgtgatgat catggcgggc gaacgtggga
    10201 tctccccaga gcgtcacgcg cagttgctgg ctgaactcgg cttagataaa ccgatgtggc
    10261 agcagtatct ccattacatt tggggcgtta tgcatggcga tctaggcatt tcaatgaaaa
    10321 gccgaattcc ggtatgggaa gagttcgtgc cgcgctttca ggccacgctg gaacttggcg
    10381 tctgcgcgat gatttttgcg accgcggtcg gcattccggt tggtgtgctg gccgcggtta
    10441 aacgcggttc cattttcgat cacacagcgg ttggcctggc gctgacaggt tattcaatgc
    10501 ctatcttctg gtggggcatg atgctgatca tgctggtttc ggtgcactgg aacctgacac
    10561 ccgtctccgg tcgcgtgagc gatatggtgt tcctcgatga ttccaatccg ttaaccggtt
    10621 ttatgctgat cgacaccgcc atctggggtg aagacggtaa ctttatcgat gccgtcgccc
    10681 atatgatctt gcccgccatt gtgctgggta ctattccgct ggcggtcatt gtgcgtatga
    10741 cacgctcctc gatgctggaa gtgctgggtg aagattacat ccgcaccgcg cgcgccaaag
    10801 ggttgacccg catgcgggtg attatcgtcc atgcgctgcg taacgcgatg ctgccggtag
    10861 tgaccgttat cggcctccag gtgggaacat tgctggcggg ggcgattctg accgaaacca
    10921 tcttctcgtg gcccggtctg gggcgctggt tgattgacgc actgcaacgg cgcgattatc
    10981 cggtggtgca gggcggcgta ttgctggtgg cgacgatgat tatcctcgtc aacctgctgg
    11041 tcgatctgct gtatggcgtg gtgaacccgc gtattcgtca taagaagtaa ggggtcatca
    11101 tgtcacaggt tactgaaaat aaagtgatta gcgcgccggt accgatgacc cctttacagg
    11161 agttctggca ctattttaaa cgcaacaaag gcgcggtcgt cgggctggtt tacgtcgtca
    11221 tcgtgctgtt tatcgcgatc tttgccaact ggattgctcc ctataacccg gcggaacagt
    11281 tccgcgatgc actgctcgcc ccgccagcct ggcaggaagg cggcagcatg gcgcacttgc
    11341 tgggtaccga tgatgtaggc cgtgatgtgc tgtcgcggct gatgtacggc gcgcgcttgt
    11401 cgctgctggt tggctgtctg gtggtggtgt tatcgctgat tatgggcgtt attctcggcc
    11461 tgatcgccgg ttacttcggc ggcctggtcg ataacatcat tatgcgcgtg gtcgatatca
    11521 tgctggcgct gccaagtctg ctgttggcac tggtgctggt ggcgattttc ggcccgtcga
    11581 ttggtaacgc cgcgctggcg ctgacctttg ttgccttgcc gcactatgtg cgcttaaccc
    11641 gcgccgccgt gctggtggag gtgaatcgcg attacgtcac cgcttctcgc gtggcaggag
    11701 ccggggcgat gcgccagatg tttattaaca tcttcccgaa ctgccttgca ccgctgattg
    11761 ttcaggcgtc gctcggtttc tctaacgcca ttctcgatat ggctgctctt ggtttcctcg
    11821 gcatgggggc gcagccgcca acgccggagt ggggcaccat gctctccgac gtgttgcagt
    11881 tcgcgcaaag cgcctggtgg gtcgtgacct tcccgggtct ggcgatcctg ctgacggtgc
    11941 tggcatttaa cctgatgggt gacggtctgc gtgacgcgct cgatcccaaa ctgaagcagt
    12001 aagaggttcg agatggcgtt attaaatgta gataaattat cggtgcattt cggcgacgaa
    12061 agcgcaccgt tccgcgccgt agaccgcatt agctacagcg taaaacaggg cgaagtagtc
    12121 gggattgtgg gtgagtctgg ctccggtaag tcggtcagtt cgctggcgat tatggggctg
    12181 attgattatc cgggccgcgt gatggcggaa aagctggagt ttaacggtca ggatttgcag
    12241 cgtatctcgg aaaaagagcg ccgcaacctg gtgggtgccg aagtggcgat gatcttccag
    12301 gacccgatga ccagccttaa cccgtgctac accgtgggtt tccagattat ggaagcgatt
    12361 aaggtgcatc agggcggtaa taagagcact cgtcgtcagc gggcgattga cttgttgaat
    12421 caggtcggta ttcccgatcc ggcttcgcgt ctggatgttt acccgcatca gctttccggc
    12481 ggcatgagcc agcgcgtgat gattgcgatg gccattgcct gtcggccaaa actgctgatt
    12541 gccgatgaac caaccactgc gctggacgtg accattcagg cgcaaatcat tgaactactg
    12601 ctggagctac agcagaaaga gaacatggcg ctggtgttaa ttacccatga cctggcgctg
    12661 gtggcggaag cggcacataa aatcatcgtg atgtatgccg gtcaggtggt ggaaacgggc
    12721 gatgcgcacg ccatcttcca tgcgccgcgt cacccgtata ctcaggcatt gctgcgtgcg
    12781 ctgccggaat ttgctcagga caaagaacgt ctggcatcgt tgcctggcgt tgttcccggc
    12841 aagtacgacc gcccgaacgg ctgcttgctt aacccgcgct gcccctatgc cactgacaga
    12901 tgtcgcgctg aagaaccggc gctgaatatg ctcgctgacg ggcgtcagtc caaatgccat
    12961 tacccacttg atgatgccgg gaggccgaca ctatgagtac gcaagaggcc acctcgcaac
    13021 aaccgctgtt gcaggctatc gacctgaaaa aacattatcc ggtgaagaaa ggtatgttcg
    13081 ctccggaacg tctggtgaag gcgctggacg gcgtttcgtt taaccttgaa cgtggcaaaa
    13141 cgctggcggt agtaggtgaa tctggctgcg gtaaatcgac cctcggtcgg ttgctgacga
    13201 tgattgaaac gcccaccggc ggcgagctgt attaccaggg gcaggatctg cttaagcacg
    13261 atccgcaggc gcagaagctg cgtcggcaga aaatccagat tgtcttccag aacccttacg
    13321 gttcgctgaa tccgcgtaaa aaaatcgggc aaattcttga agagccgctg ctgatcaaca
    13381 ccagcttaag caaagaacag cgtcgggaaa aagccctgtc gatgatggcg aaagtcggcc
    13441 tgaaaaccga gcactatgac cgctatccgc atatgttctc cggcggtcag cgtcagcgta
    13501 tcgccattgc ccgaggtctg atgctcgacc cggacgtggt gattgccgat gaaccggtct
    13561 ccgcgctgga cgtctcggtg cgtgcacagg tgctcaacct gatgatggat ttgcagcagg
    13621 aactggggct ttcttatgtc ttcatctccc acgacctgtc ggtggtggag cacattgctg
    13681 atgaagtgat ggtgatgtac ctgggccgct gcgtggagaa gggaacgaaa gaccaaatct
    13741 tcaataaccc gcgccatccg tacactcagg cgctactttc cgcgacgccg cgcctgaatc
    13801 cggacgatcg ccgcgagcgc atcaaactca ccggtgaact gccaagcccg ctgaatccac
    13861 cgccgggctg cgcgtttaac gcccgctgtc gtcggcgctt cggcccctgc acccagttgc
    13921 agccgcagct aaaagactac ggcggtcaac tggtagcttg ttttgctgtt gatcaggatg
    13981 aaaatccgca gcgttaactc acaaaaccgg ggccatgcgc tccggtttac tcaatcagct
    14041 tcaacagtgg cgacaagcat agcaaaatgc cgtaaagcag aatcagccag gttttaaatc
    14101 cgcgaagttt ttccagttgt gcgactttat agatcaggaa aaacggaatg aggcacgaca
    14161 caatgccata taacgggctt cccaactgaa agaacaccag caccgataca cgaaacgaaa
    14221 cccaaatcgt caacgtaatg acgataaaag cgcagatcgc cagagtcagc acgcgtgagt
    14281 taattttctt ggtatcaata attcggctta acagattgag aataatgcct ttaatggcct
    14341 cgtggaaacc gagataaatg ccaaagaatg cggtcagtac ggcaaagata ttcagcaccg
    14401 tagaggtgat atgaatgata tgcccaggga tcacctgcgc ggccagtgcc agtgccgaga
    14461 tattttgttc aaaggcagaa acggcttctt cgtggctaat tgagaaggta aacgaaaagg
    14521 caaaaaacag tatcaccgcg atgagcgtga tataactaat tcggtgggta cgcagcgcga
    14581 gccgggttgc cagtacttta tccgcttccc gtttacgata ggcaatattc attgggttaa
    14641 gtacctgaat aaatactgca gaaaagaagc aaaatggaat ggtaagcagg acatcgcgaa
    14701 aaaagacgga ggcttgcggg aaggcggtta tattggcgaa attccagtgc gggatcatcg
    14761 taaaaccgaa cacgacaata atccctactt tgaccaccac cattggccca gaaatcttaa
    14821 acagtaatcg ttcaccacca gacgcaatcg ctaccagtac ggcgaaaata gcgactttat
    14881 aaagtagaga ttgtgaaaga tcggcatcgg ttaaaccgaa ggttttcagg tacgaggcgc
    14941 tgtcgaaaac cacggagaga gagtagataa aaatcccgtg gataatcatc aaaaagtaga
    15001 taaccccgag gaaaattccc cagttcttcc ccaggtaatg actgataata tcggtgtagt
    15061 cattacagga atcgctttca gaaagggttt ttaaataaat gtcctgcact acccaggtgg
    15121 caggataagc aatgatcgct gcggtaataa atacccaaat gcccttcaag ccaatttgta
    15181 ctggcatcag cacggttccg gcaccaatcg ccatgcctat gcataataaa acccagccga
    15241 agtcgtagcg tgtaaacggc aatttctgat cgtgtttcgg tagtgtgttg tgctgcataa
    15301 agtcctgtcc agaaagaagt cggcctcgca aaaatgaata aacccagttt ctcttgcgca
    15361 acaggtggag aagtcgaata cgcagtatgt aaaaaatgag gcaacagatt cactggggag
    15421 gtcgcgagga ggcgcaattc tacaaaattg cgcatttcac taccagtctg gagtcagccc
    15481 ggatatgaac tgcaactatt gcaaattaag aaattaagct ggaaggcatc acgcaatgag
    15541 gaagattgcc gagtgctgac agtaaaaagc aatcgtaaaa ttagcggcaa gccggaaggg
    15601 ttccggtgag gcgcaatgtt gcgggggctt aatccctggt ggcattggtt gctggagaga
    15661 gaaaaccccc gcacgttgca ggtatgcacc tgacaacacc acgggggcta atcttgaacc
    15721 tagaccactc aagaatagcc gcgaaacgtt gtcattacaa cacaggcggc tatatgacgc
    15781 tcgcagagct gggcatggcc ttctggcatg atttagcggc tccggtcatt gctggcattc
    15841 ttgccagtat gatcgtgaac tggctgaaca agcggaagta acgtgtcatg cgggcgtcag
    15901 gctgccgtaa tggcaatttg cgcccggacc agaccgcagg ggggaaactc tgcggccttt
    15961 ttcgtgctta ctgcgggtaa ggcacccagt cgccgccgtt caggcgaacg tacggtttat
    16021 cctggtattg aataactact gcatttgagt tctctgagac cggtgctgtt tgtggcaacc
    16081 cactggtgag ttttttccag tcaacattgt cttcggtgaa aatcttgcca tcaagaacgc
    16141 gaaccaccag atcggagata gccaggaagc tgctcggttg gtcgatgaca atcggtgccc
    16201 cctgatgtgg tgccttcatg ccgaagaatt tcaccccaac ggggacgtcg gtgatagacg
    16261 ggctagggat atcacgtagg ccagatacct gcattctgtc gcccttcagc gcgccgccgt
    16321 gttccggcac cacgaccacc atcactttac gacccgattt ctccagttca gtaaagaagg
    16381 cgtccagttc atcaaagaat ttctgcgccc gcgctttgta atccgctgtt ttgctgacgc
    16441 ccggataatg gttgccgtca tgcagtggaa gcgtgttgta gaacgtggca ctacggctgt
    16501 ttttatcttt ttcggtaacg tccagccagc ggttaagcac ggcggtatcg tcataaaccg
    16561 gcgaaccatc aaagcccagc aaaataaccg gcagatttgt ttgatccatc aattcagtct
    16621 gcatgccgcc attttcgcga acttctttca aaaaaccgcc gaactggccg ttatgtccca
    16681 tcatcaggtg ctgggtaaag cccagtttcg acaggttatc aaacagatag cagtcgttat
    16741 ttgccggttg atacagatta gtgtgcgaag tctgcccgca gctggcgcgc agtaaacgga
    16801 tcgccgccgg gccactgtag gaggtggcgg agttaaagtt cttgaactca atatcgaaat
    16861 gcgaccacag tggatgcgac atcaacccag cggcttctat atccgaccag gaaagcgaac
    16921 agatgttaat caccagtagt tcaaatggct gagcatcagc gggcagcgaa gacgggaagg
    16981 tcgatttacg tttcgcctcc gcgttataga aattattcag ccaggcgtta aggttcgccg
    17041 ttgttggcgg tgcagtttgt gcgggcatat cacccactac cggtgcgcca cccgtcgccg
    17101 caacggttgc cgctgcgtta ccactcgtcg ttgttacagt ggtcgtcggt tgtccggctg
    17161 gccacaagga gaaacttggt cccgccaggg taagtacgtt cagccatagc agtatggcaa
    17221 ccacaaaaac ggtaatgcga atccattgtg acaggaataa ccaggccact aataaaacaa
    17281 aaatggcccc aatcatctgc cagttaataa agcgtgtgac aaggtcgatt aaataatcgg
    17341 tactgaaccc cgccacctgc gaaccctggc tcattatgct ttccaggcca ggcaaccagg
    17401 tgtcatgcca gaacaaagca aagccgatcg gcagggcaat ccagtggcgc aagcgatgca
    17461 ggctgtagcg cggaatgggc atcagcagaa acgcggcaaa caccaaattg aggagcggat
    17521 ggaagttaag atatcccgcc cacaacaggc cgaacttaac cagaaaatag aagttccagc
    17581 cggaaaggcc gcgccagtat tgccagaggg aagaaggcat ggcggtattt tgcgtaaatt
    17641 gagtcatttt ttggttgccc tggctttttc cgtgcggcgt aacgtcccag ccggtttaac
    17701 ataacgaggt ttagcaaaga acaaacgtaa ggtgtcgcga atgcgtcgca aagagtgccg
    17761 cgccagatag cccagcggga aaaatatcag tgcgcaaacg acaataattt caatgatatc
    17821 gctgatggtc atcatgatga gcgctccaca gcatcatcta acagtcgcat gggttctggt
    17881 attcgtcgcc agtggcgacc atcgtgctcg gcattgatga ccggtttaga actttgcgtt
    17941 aaaggcagcg gcatgcccca ttgttctggg gcaagcaggc gcatctgcac cagctcggca
    18001 ctgatttgat catcttcaaa ccagaccata cggtttgaga aaatgtcgcc agtaggcaat
    18061 gggaaaatat gattcaacgc ggtatccaga tcgttaatcc gacagaatga gagaaacagc
    18121 accagccgat taccgccaat ggtcatgata tcgccggtac ggttagggcg acacagcgtc
    18181 agggcttgtt caacgcggat acccggtacc ggacgtaggg caaccagaac gcctttaccg
    18241 tgggcaggta atagagggtt attcatcatg ttgttgacgg cattacagaa cacatcccac
    18301 ttctggaaac cacgcagttt gagcggctgg gtcattgaca gcaaggtagt gatatcttcc
    18361 ggcacatagc gactaaactt ctgcccttgc acgctttcga tcatcgtcag acaacgggag
    18421 agtggcgcat tccacggaat aaccatattt gcaccgcagg ccaataacaa acgttcatcg
    18481 gtggcgcgca ggctagcggt attttcccgc acgaggattt tcatcgcact accgcgctgg
    18541 cgacgcaggg tatgaatgct gcgggccagt ggctcgattt gcgcattttg ctgtaaagaa
    18601 aagaccaccg tcgccgcctg agcggtacgg gcttcattga acaggacttc gttattgttg
    18661 aacagttgcc agtgttccga tagcggcggc gcaccttcca gtacagcaac attactcaga
    18721 atgcgttttt cgtcgctgcg tggttggatc tccgcctctt cgctttgaac taatgtccag
    18781 ataccatttt gttgctgaac gctaagctgc tgacgggcgc tgaccccttt ttcgttgcac
    18841 cagaaggcaa tatccagcaa atgttggtcg ccctgaaaac gcaaactggc aagaccaaaa
    18901 agtgaacggt attcctcaag caacaatgaa aattgtttat cgttattatt tccgggatta
    18961 attaccaaaa gcgaacaatg gtttaacctg ctccatttat tcattttatc caaccatgag
    19021 cgcagccgct cggcaggaat gttttgccat gcgttatttg cgcaaacaag aataaaaagg
    19081 taattatggg gatcaataga acactgcaaa tcacgggcca aatagtatag acctttttca
    19141 tgatttagca ttgaaaataa ttttattttt tccggccctt gagaatcatc taattgaaag
    19201 attttcgccg gatcgctgtc catgctaatg accgcgacgt gtgcggtttc agcctgggat
    19261 gcaattgttt gattcgccag actgatagca tcttcatggc gatcgacgtt aaaccaccag
    19321 acgccgcctg ctggcatatg tcgcagctca tcccataatg atgagatacc gatagagaat
    19381 acagggtcca caatgtccct catgtcacca ttttgtctga ttatcagttt actagcgaaa
    19441 gctaagaaat aaacctacca ttgaaattaa aacttatcag atttagcatg tgaatcaatt
    19501 tagcaggtca caagacgtgt gattttagtc ttttcgtttt taactttttc tgttgcgtaa
    19561 cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa acaataaacc ggcgaaatgc
    19621 tcacggttag aattgtctca tgaacggtac ggttatttca tagggatcaa gcaaaatgaa
    19681 taacaatgaa ccagatactc tgcctgatcc cgcgataggc tatatcttcc agaatgatat
    19741 tttggcgtta aagcaggcat tttcactgcc tggtattgat tatgccgata tttcccaacg
    19801 cgaacagttg gccgcggcat taaaacgctg gccgttgctg gcagagtttg cgcaacaaaa
    19861 gtaggggatt ggtgaatggc cgtactggga ttgcaggggg tgcggggagg cgtggggaca
    19921 acaaccatca ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgtcctggtg
    19981 gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta atgttgattt tacccaccgt
    20041 cagggctggg ccagagctat gctggatggc taggactggc gcgacgctgg gttgcgctac
    20101 acctcgcagc tcgatttgct gccttttggt cagttatcca ttgaagaaca agaaaatcca
    20161 cagcactggc aaacccggct gagcgatatt tgctccggct tacagcaact aaaagccagc
    20221 gggcgttacc agtggatttt aatcgactta ccgcgtgatg cctcgcagat aacccaccag
    20281 ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg tggatgccaa ctgccatatc
    20341 cgactgcatc agcaagcgct gccggatggc gcacatattt tgattaataa cttccgtatt
    20401 ggcagtcagg ttcaggacga tatttaccag ctttggttgc aaagccagcg ccgattactg
    20461 ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc tggcggctaa gcaaccagta
    20521 ggtgaatatc gcagtgatgc gctggcggct gaagagatat tgacgctggc gaactggtgc
    20581 ctgttgaact attccgggct gaaaacgcca gtcgggagtg catcatgagt atcctgaccc
    20641 ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg gcgttatcgc gattatcgtc
    20701 gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt ctggatgatc ctggcctgga
    20761 tttttattcc actggagcac ccgcgctggc agcgtattcg cgcagaacat aaaaacctgt
    20821 atccgcatat caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat ctcattcaaa
    20881 catgctggtt actgatcggt gcatcgcgca aagaaacgcc gaaaccgcgc aggcgggcat
    20941 tttcaggtct gcagaatatt cgtggacgtt accatcaatg gatgaacgag ctgcctgagc
    21001 gcgttagcca taaaacacag catcttgatg agaaaaaaga gctcggtcat ttgagtgccg
    21061 gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc gctgattctg gcgttaatct
    21121 gcgttactca gccgtttaac ccgctggcgc agtttatctt cctgatgctg ctgtgggggg
    21181 gagcgctgat cgtacggcgg atgccggggc gcttctcagc gctaatgttg attgtgctgt
    21241 cgctgactgt ttcttgccgt tatatctggt ggcgatatac ctctacgctg aactgggacg
    21301 atccggtcag cctggtgtgc gggcttattc tgctcttcgc tgaaacgtac gcgtggattg
    21361 tgctggtgct cggctacttc caggtagtat ggccgctgaa tcgtcagccg gtgccattgc
    21421 cgaaagatat gtcgctgtgg ccgtcggtgg atatctttgt cccgacttac aacgaagatc
    21481 tcaacgtggt gaaaaatacc atttacgcct cgctgggtat cgactggccg aaagacaagc
    21541 tgaacatctg gatcctcgat gatggcggca gggaagagtt tcgccagttt gcgcaaaacg
    21601 tgggggtgaa gtatatcgcc cgtaccactc atgaacatgc gaaagcgggc aacatcaaca
    21661 atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt cgactgcgac cacgtaccaa
    21721 cgcgatcgtt cctgcaaatg accgtgggct ggttcctgaa agagaaacag ctggcgatga
    21781 tgcagacccc acaccatttc ttctcgccgg acccgtttga acgcaacctg gggcgttttc
    21841 gtaaaacacc gaacgaaggc acgctgttct atggtctggt gcaggatggc aacgatatgt
    21901 gggacgccac tttcttctgc ggttcctgtg cggtgattcg ccgtaagccg ctggatgaaa
    21961 ttggcggcat tgctgtcgaa actgtgactg aagatgcgca tacttctctg cgtttgcacc
    22021 gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc ggcggggctg gcgaccgaaa
    22081 gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg cgggatggtg caaatcttcc
    22141 gtctcgataa cccgctcacc ggtaaagggc tgaagtttgc tcagcggctg tgctacgtca
    22201 acgccatgtt ccacttcttg tcgggcattc cacggctgat cttcctgact gcgccgctgg
    22261 cgttcctgct gcttcatgcc tacatcatct atgcgccagc gttgatgatc gccctgttcg
    22321 tgctgccgca tatgatccat gccagcctga ccaactccaa gatccagggc aaatatcgcc
    22381 actctttctg gagtgaaatc tacgaaacgg tgctggcgtg gtatatcgca ccaccgacgc
    22441 tggtggcgct gattaacccg cacaaaggca aatttaacgt caccgccaaa ggtggactgg
    22501 tggaagaaga gtacgtcgac tgggtgatct cgcggcccta catcttcctt gttctgctca
    22561 acctggtggg cgttgcggta ggcatctggc gctacttcta tggcccgcca accgagatgc
    22621 tcaccgtggt cgtcagtatg gtgtgggtat tctacaacct gattgttctt ggcggcgcag
    22681 ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca ccgcgtggag atgacgatgc
    22741 ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg taccgttcag gatttctccg
    22801 acggtggttt ggggatcaag atcaacggtc aggcgcagat tctggaaggg cagaaagtga
    22861 atctgttgct taaacgcggt cagcaggaat acgtcttccc gacccaggtg gcgcgcgtga
    22921 tgggtaatga agttgggctg aaattaatgc cgctcaccac ccagcaacat atcgattttg
    22981 tgcagtgtac gtttgcccgt gcggatacat gggcgctctg gcaggacagc tacccggaag
    23041 ataagccgct ggaaagtctg ctggatattc tgaagctcgg cttccgtggc taccgccatc
    23101 tggcggagtt tgcgccttct tcggtgaagg gcatattccg tgtgctgact tctctggttt
    23161 cctgggttgt atcgtttatt ccgcgccgcc cggagcggag cgaaacggca caaccatcgg
    23221 atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa gaaaactatt ctggatttgt
    23281 gcagtggcta tggggatgag tgcgttcccc tctttcatga cgcaggcgac gccagcaacg
    23341 caaccactga tcaatgctga gccagctgta gccgcccaga cggaacaaaa tccgcaggtg
    23401 gggcaagtga tgccgggcgt gcagggcgct gatgcgccag tcgtggcgca gaacggtcct
    23461 tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc cgccgggcag catggtgcta
    23521 cgtggcatta acccgaacgg cagcattgag tttggtatgc gcagcgatga agtggtgacg
    23581 aaggcgatgc tcaacctcga atacacgcca tcgccatcgt tactgcctgt ccagtcgcag
    23641 ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc cagtgaccaa agaacagttg
    23701 ggtaaaaaaa cgctggcgca aatgcccatt aatccactgt ttattaccga cttcaaccgt
    23761 gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg aaaacccggc cagcaccacg
    23821 ctttggctgg atgttgggcg gagcagtgga ctggatctga cctatcagac cctgaatgtg
    23881 aagaatgacc tgtcacactt cccggtgcca ttctttgacc cgcgtgataa ccgcaccaac
    23941 accttgccga tggtctttgc gggtgcgccg gatgttgggc tgcaacaagc atctgccatt
    24001 gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc agaacttccc ggtgctctat
    24061 aaccaactgc cggatcgtaa tgccattgtc tttgcaacca acgacaaacg gccggacttc
    24121 ctgcgcgatc atccggcggt aaaagccccg gtgattgaga tgattaacca tccgcagaat
    24181 ccttacgtca aactgctggt ggtgtttggt cgtgacgaca aagacctgtt gcaggcagcg
    24241 aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa gcgtggtagt gaatgaagtg
    24301 aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact gggtgcgtac cgatcgtccg
    24361 gtcacctttg gtgaactgaa aacctatgaa gaacagttac aatccagcgg tcttgagcca
    24421 gcagcgatta atgtttcgct aaacctgccg ccagatctct acctgatgcg cagtaccggc
    24481 attgatatgg atattaatta ccgctacacc atgccgccgg tgaaagacag ttcgcggatg
    24541 gatatcagcc tgaataacca gttcctgcaa tccttcaacc tgagcagcaa acaggaggcg
    24601 aaccgcctgc tgctgcggat tccggtatta caaggtttgc tggatggcaa aacggatgtc
    24661 tctattccgg cgctgaaact gggcgcgacc aaccagctgc gcttcgactt tgagtatatg
    24721 aacccgatgc ctggcggttc ggtggataac tgtattacct tccagccggt gcagaatcat
    24781 gtggtgattg gtgacgactc caccatcgac ttctcgaagt attaccactt catcccgatg
    24841 ccggatctac gcgcctttgc taacgcgggc ttcccgttca gccggatggc ggatctgtcg
    24901 caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac agatggaaac gttgctgaat
    24961 actgttggtt ttatcggcgc acagacgggc ttcccggcga ttaatctgac ggtgaccgat
    25021 gatggcagca ccattcaggg caaagatgcc gacatcatga tcatcggtgg tatcccggac
    25081 aaactgaaag acgataagca gatcgaccta ttggtgcagg cgaccgaaag ctgggtgaaa
    25141 acaccgatgc gccagacccc gttccccggc attgtgccgg acgagagcga tcgcgcggca
    25201 gaaacccagt caacgctgac ctcttccggt gcgatggcgg cggtgattgg cttccagtcg
    25261 ccgtataacg accagcgcag cgtgattgcg ctgctggcag atagcccacg cggttatgaa
    25321 atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca ccatgttcgg ttcggtcgcg
    25381 gtgatccgcg agtccggtat caacagccta cgtgttggcg acgtttatta cgtaggtcat
    25441 ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc atccgattct gctggcggtg
    25501 ctggcggcta tcagtgtgat attgctggca tgggtactgt ggcgtttgct gcgtattatc
    25561 agccgtcgtc gtcttaaccc ggataacgag taattgaaga tgaatgtgtt gcgtagtgga
    25621 ctcgtgacga tgctgctgtt ggctgccttt agtgttcagg cagcctgtac ctggcctgcc
    25681 tgggagcagt ttaaaaagga ttacatcagt caggaagggc gcgtcatcga ccccagcgac
    25741 gcgcgcaaaa tcaccacctc tgaagggcaa agttacggca tgttctttgc cctggcggct
    25801 aacgaccgtg cagctttcga taatattctc gactggacgc agaacaatct cgctcagggt
    25861 tctttaaaag aacgtttgcc cgcctggctg tggggcaaga aagagaacag taagtgggaa
    25921 gtgctggaca gcaattcggc ctccgatggt gatgtctgga tggcttggtc gttgctggag
    25981 gcggggcgtt tgtggaaaga gcagcgttat accgacatcg gcagcgcgtt gctaaaacgt
    26041 attgcgcggg aggaagtggt gacggtgcct gggctgggtt ccatgttgtt accgggcaaa
    26101 gtgggttttg ctgaggataa cagctggcgt tttaacccca gctacctgcc gccgacgctg
    26161 gcgcagtatt tcacccgctt tggcgcgccg tggactacgc tgcgcgaaac caatcaacgt
    26221 ttattgctgg aaaccgcccc gaaaggcttt tcgccagact gggtgcgcta tgagaaagac
    26281 aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca gctacgacgc tatccgcgtt
    26341 tacatgtggg taggcatgat gcctgacagc gatccgcaga aagcgcggat gctcaaccgg
    26401 tttaaaccga tggcgacatt cactgagaaa aacggttatc cgccggaaaa agtggatgtg
    26461 gctacgggga aagcgcaggg taaaggaccg gtcggttttt ctgccgccat gctgcccttt
    26521 ttacaaaacc gtgatgcgca ggccgttcag cgccagcgcg tggccgataa ctttcccggc
    26581 agcgatgcct attacaacta tgtgctgacc ctgtttggac aaggctggga tcaacaccgt
    26641 ttccgcttct cgacaaaagg tgagttatta cctgactggg gccaggaatg cgcaaattca
    26701 cactaaacat attcacgctt tccctcggtc tggccgtcat gccgatggtc gaggcagcac
    26761 caaccgctca gcaacagttg ctggagcaag ttcggttagg cgaagcgacc catcgtgaag
    26821 acctggtgca gcagtcgttg tatcggctgg aacttattga tccgaataac ccggacgtcg
    26881 ttgccgcccg tttccgttct ttgttacgtc agggcgatat tgatggcgcg caaaaacagc
    26941 tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta taaatcgtcg cggactacga
    27001 tgctactttc cacgccggat ggtcgtcagg cactgcaaca ggcaagattg caggcgacta
    27061 ccggtcatgc ggaagaagct gtggcgagtt acaacaaact gttcaacggt gcgccgccgg
    27121 aaggtgacat tgctgtcgag tactggagta cggtggcgaa aattccggct cgccgtggcg
    27181 aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc gggcaatacg ggcctgcaaa
    27241 acaatctggc gctattgctg tttagtagcg atcgccgtga cgaaggtttt gccgtcctgg
    27301 aacagatggc gaaatcgaac gccgggcgcg aaggggcctc taaaatctgg tacgggcaga
    27361 ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct gaaaaaatat ctttctatct
    27421 ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact ggcagaacag caaaaacagc
    27481 tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc ggcggtggac tctggtatgg
    27541 cgggtaaagc cattcccgaa ctacaacagg cagtgcgggc aaacccgaaa gacagtgaag
    27601 ctctgggggc gctgggccag gcatattcac agaaaggcga tcgcgccaat gcagtggcga
    27661 atctggaaaa agccctcgca ctggatccgc acagcagcaa caacgacaaa tggaacagtc
    27721 tgctgaaagt aaaccgctac tggctggcga tccagcaggg cgatgctgcg ctgaaagcca
    27781 ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg taatgtcgat aacaccgaca
    27841 gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg aaaagattat cccgccgccg
    27901 aacgttatta tcagcagacc ttgcgtatgg acagcggcaa cactaacgcc gtgcgcgggc
    27961 tggcaaatat ttaccgccag caatcgccag aaaaagctga agcgtttatc gcctcactct
    28021 ctgccagtca gcggcgtagc attgatgata tcgaacgcag cctgcaaaac gaccgtctgg
    28081 cacagcaggc agaggcactg gaaaaccagg gcaaatgggc gcaggcggca gcacttcagc
    28141 ggcaacgact ggcgctggac cccggcagcg tatggattac ttaccgactt tcgcaggatc
    28201 tctggcaggc cggacaacgc agccaggccg atacgttaat gcgcaatctg gcgcagcaga
    28261 agccgaacga cccggagcag gtttacgctt acgggctgta cctctctggt catgaccagg
    28321 acagagcggc gctggcgcat atcaacagcc tgccgcgtgc gcagtggagc agcaatattc
    28381 aggagctggt taatcgactg caaagcgatc aggtgctgga aaccgctaat cgcctgcgag
    28441 aaagcggcaa agaagctgaa gcggaagcga tgctgcgtca gcaaccacct tccacgcgta
    28501 ttgacctcac gctggctgac tgggcgctgc agcgacgtga ttacaccgcc gcccgcgctg
    28561 catatcagaa tgtcctgacg cgggagccaa ctaacgccga tgccattctt ggtctgacgg
    28621 aagtggatat tgctgccggt gacacagcgg cggcacgtag ccagctggcg aaactgcccg
    28681 ctactgataa cgcctcgctg aacacacagc ggcgcgtggc gctggcgcag gcgcagcttg
    28741 gagataccgc agcggcgcag cagacgttta ataagttgat cccgcaggca aaatctcagc
    28801 caccgtcgat ggaaagcgcg atggtgctgc gcgacggtgc gaagtttgaa gcgcaggcgg
    28861 gcgatccaaa gcaggcgctg gaaacctaca aagacgccat ggtcgcatcc ggtgtgacta
    28921 cgacgcgtcc gcaggataac gacaccttta cccgactgac ccgtaacgac gagaaagatg
    28981 actggctgaa acgcggcgtg cgcagcgatg cggcggacct ctatcgccag caggatctta
    29041 acgtcaccct cgagcatgat tattggggtt cgagcggcac cggtggttac tccgatctga
    29101 aagcgcacac taccatgttg caggtggatg cgccgtattc tgacgggcgg atgttctttc
    29161 gcagtgattt cgtcaatatg aacgtcggca gtttctccac taatgccgat ggcaaatggg
    29221 atgacaactg gggcacctgt acattacggg actgtagcgg caaccgcagc cagtcggatt
    29281 ccggtgccag cgtggcggtc ggctggcgaa atgacgtctg gagctgggat atcggcacca
    29341 cgccgatggg cttcaacgtg gtggatgtgg tcggcggcat cagttacagc gatgatatcg
    29401 ggccgctggg ttacaccgtt aacgcccacc gtcgtcccat ctccagttct ttgctggcct
    29461 ttggtgggca aaaagactcc ccgagcaata ccgggaaaaa atggggcggc gtgcgtgccg
    29521 acggcgtggg gctaagcctg agctacgata aaggtgaagc aaacggcgtc tgggcatcgc
    29581 ttagtggcga ccagttaacc ggtaaaaatg tcgaagataa ctggcgtgtg cgctggatga
    29641 cgggctatta ctataaggtc atcaaccaga acaatcgccg cgtcacaatc ggcctgaaca
    29701 acatgatctg gcattacgac aaagacctga gtggctactc actcggtcag ggcggttact
    29761 acagcccgca ggaatacctg tcgtttgcca taccggtgat gtggcgggag cgcacggaaa
    29821 actggtcgtg ggagctgggg gcgtctggct cgtggtcgca ttcacgcacc aaaaccatgc
    29881 cgcgttatcc gctgatgaat ctgatcccga ccgactggca ggaagaagct gcgcggcaat
    29941 ccaacgatgg cggcagcagt cagggcttcg gctatacggc gcgggcatta cttgaacgac
    30001 gtgttacttc caactggttt gtcggcacgg caattgatat ccagcaggcg aaagattacg
    30061 cccccagcca tttcctgctc tacgtacgtt attccgccgc cggatggcag ggtgacatgg
    30121 atttaccgcc gcagccgctg ataccttacg ccgactggta agttttcaga tagcgcctct
    30181 cttaatgccg ctgcgatcgg gtatactcgg gcggcaatct gggatttccg gggggagaca
    30241 atttgcgcgt aagtcgctcg ttaacaatca agcagatggc aatggtggca gccgttgtcc
    30301 tggtgttcgt ttttattttt tgcaccgttt tgctgttcca tctggtccag cagaatcgct
    30361 ataacacggc tacgcaactg gaaagcattg ctcgctctgt ccgcgaacct ttatcttcag
    30421 ccattttgaa aggtgatatt cccgaagcgg aagctattct tgccagcatt aaaccggcag
    30481 gcgtggtcag ccgtgccgat gtagtgctgc ctaaccagtt ccaggcgctg cgtaaaagtt
    30541 ttattccaga gcgtccggtg ccggtaatgg ttactcgcct gtttgagcta ccggttcaaa
    30601 tctcgctggg cgtctactcg ctcgaacgtc cggcaaatcc gcagccaatt gcctatctgg
    30661 tgctacaggc ggattccttc cgtatgtata agttcgtgat gagcaccctc tcaacgttag
    30721 tgaccattta cttacttttg tcgttaatat tgacggtggc gattagctgg tgcattaacc
    30781 gcctgatttt gcatccgtta cgcaatattg ctcgcgaact taacgccatc ccagcccagg
    30841 agcttgttgg tcaccaactg gcattaccgc gtctgcatca ggacgatgaa atcggtatgt
    30901 tggtgcgcag ttacaacctc aaccagcaat tgctgcagcg ccattatgaa gaacagaacg
    30961 aaaatgcgat gcgcttcccg gtgtcggatt tgccgaacaa agccttgctg atggagatgc
    31021 tggagcaggt tgtcgcgcgt aaacaaacca ccgcgctgat gatcatcacc tgtgaaactc
    31081 tgcgtgatac tgcgggcgtg ctgaaagagg cgcaacgaga aattctgctg ctgacgctgg
    31141 tggaaaaact caaatcggta ctgtcgccac gtatgatcct cgcgcagatt agcggttatg
    31201 actttgctgt cattgccaac ggtgtacatg aaccgtggca cgcaatcact ttgggtcagc
    31261 aagtgctcac tatcatgagc gagcgcctgc cgattgaacg tattcaactc cgtccgcact
    31321 gtagcattag cgtggcgatg ttctacggcg atctcaccgc cgaacagctt tacagtcgcg
    31381 ctatttctgc ggcatttacc gctcgccata aaggcaagaa tcagattcag ttctttgatc
    31441 cgcagcagat ggaagccgcc cagaagcggt tgacggaaga gagcgatatc cttaatgcac
    31501 tggaaaatca tcagtttgct atttggttac agccacaggt cgagatgacc agcggtaaac
    31561 tggtcagtgc ggaagtgtta ctgcgtatcc agcaaccgga tggcagttgg gacctgccgg
    31621 atggcttaat cgatcgcatt gagtgctgtg ggctgatggt taccgtcggt cactgggtgc
    31681 tggaagagtc ctgtcgattg cttgcagcct ggcaagagcg cggcattatg ctgcccttgt
    31741 cggtaaacct ctctgtgctg caactgatgc acccgaatat ggtggcggat atgctggaac
    31801 tgttaacccg ctatcgcatt cagccgggaa cactgattct ggaagtgaca gaaagccgac
    31861 gtattgacga ccctcatgct gcggtggcaa tcctccgtcc gctgcgtaat gccggagttc
    31921 gggtggcgct ggatgatttc ggcatgggct acgcagggct gcgtcagctg cagcatatga
    31981 aatcgttgcc aatcgacgtt ctgaaaatcg acaaaatgtt tgttgaaggc ttgccggaag
    32041 atagcagcat gattgctgca attatcatgc tggcgcagag cctgaactta caaatgattg
    32101 ccgaaggcgt ggagactgaa gcacaacgcg actggctggc aaaagcgggc gttggtattg
    32161 cccagggctt cctttttgct cgtccactcc ctattgaaat cttcgaagag agttacctgg
    32221 aagaaaagta gctaccccaa actgattaca aaactttaaa aagtgctggt ttgtgcgagc
    32281 cagctcaaac tttttaacct ttttgtttca attatgatcc aggtacattt ctgtgatgtt
    32341 gtctgggtgt tattttaagg ccgcaggtac cccataacct tacaagacct gtggttttac
    32401 taaaggacac cctatgaaaa cctctctgtt taaaagcctt tactttcagg tcctgacagc
    32461 gatagccatt ggtattctcc ttggccattt ctatcctgaa ataggcgagc aaatgaaacc
    32521 gcttggcgac ggcttcgtta agctcattaa gatgatcatc gctcctgtca tcttttgtac
    32581 cgtcgtaacg ggcattgcgg gcatggaaag catgaaggcg gtcggtcgta ccggcgcagt
    32641 cgcactgctt tactttgaaa ttgtcagtac catcgcgctg attattggtc ttatcatcgt
    32701 taacgtcgtg cagcctggtg ccggaatgaa cgtcgatccg gcaacgcttg atgcgaaagc
    32761 ggtagcggtt tacgccgatc aggcgaaaga ccagggcatt gtcgccttca ttatggatgt
    32821 catcccggcg agcgtcattg gcgcatttgc cagcggtaac attctgcagg tgctgctgtt
    32881 tgccgtactg tttggttttg cgctccaccg tctgggcagc aaaggccaac tgatttttaa
    32941 cgtcatcgaa agtttctcgc aggtcatctt cggcatcatc aatatgatca tgcgtctggc
    33001 acctattggt gcgttcgggg caatggcgtt taccatcggt aaatacggcg tcggcacact
    33061 ggtgcaactg gggcagctga ttatctgttt ctacattacc tgtatcctgt ttgtggtgct
    33121 ggtattgggt tcaatcgcta aagcgactgg tttcagtatc ttcaaattta tccgctacat
    33181 ccgtgaagaa ctgctgattg tactggggac ttcatcttcc gagtcggcgc tgccgcgtat
    33241 gctcgacaag atggagaaac tcggctgccg taaatcggtg gtggggctgg tcatcccgac
    33301 aggctactcg tttaaccttg atggcacatc gatatacctg acaatggcgg cggtgtttat
    33361 cgcccaggcc actaacagtc agatggatat cgtccaccaa atcacgctgt taatcgtgtt
    33421 gctgctttct tctaaagggg cggcaggggt aacgggtagt ggctttatcg tgctggcggc
    33481 gacgctctct gcggtgggcc atttgccggt agcgggtctg gcgctgatcc tcggtatcga
    33541 ccgctttatg tcagaagctc gtgcgctgac taacctggtc ggtaacggcg tagcgaccat
    33601 tgtcgttgct aagtgggtga aagaactgga ccacaaaaaa ctggacgatg cgctgaataa
    33661 tcgtgcgccg gatggcaaaa cgcacgaatt atcctcttaa tctcatcact tatgcccgca
    33721 ctcccttccc cgagtgcggg tgtttgcgca taattgaccc ctgtgcctga acttaccacc
    33781 tgggtgagtt caggtttatt tcacttcttc cgtgacattt tcatgttctt gcggtctaac
    33841 acgaagtgtt tttaacgtca tttagactga ccgacaacgg gcagtttttt tattaccagg
    33901 atagttgatc aggggttcac atgcagggca caaaaattcg acttttagcg ggcggtttgc
    33961 tgatgatggc cactgctggc tatgtgcagg cagatgcgct ccagcctgat ccagcatggc
    34021 aacaggggac gctttccaac ggtttacagt ggcaagtgct gaccaccccc cagcgtccca
    34081 gcgatcgtgt tgaaattcgc ctgctggtta ataccggttc gctcgccgaa agtacacaac
    34141 agagcggtta cagtcacgcc atccctcgta ttgcgctaac gcaaagcggt ggccttgacg
    34201 cagcacaggc gcgttcattg tggcagcagg ggatcgaccc taaacgcccg atgccgccgg
    34261 taattgtctc ttatgacacc acgctgttta atctgagttt gcccaataac cgtaacgatt
    34321 tgctgaaaga ggcgctctct tatctggcaa atgccactgg caaactgact atcacgccag
    34381 aaaccatcaa ccacgcgctg caaagtcagg acatggtggc aacctggcct gccgatacta
    34441 aagagggctg gtggcgctat cgtctgaaag ggtcaacctt gttaggtcac gatcctgccg
    34501 atccgctgaa acaacccgtt gaagcggaaa aaattaaaga tttctatcag aaatggtaca
    34561 ccccggatgc aatgacgctg ctggtggtgg gaaacgtgga tgcgcgctcg gtcgtcgacc
    34621 aaatcaacaa aacgtttggc gaactgaaag gcaaacgtga aacaccggct ccggtgccga
    34681 cgctttctcc gctgcgtgcg gaagcggtga gtattatgac tgacgcggtg cgtcaggacc
    34741 ggttatctat catgtgggat acgccgtggc agccgattcg tgaatcagcc gcactgctgc
    34801 gctactggcg tgcggacctg gcccgtgagg cgctgttctg gcatgttcag caagcgttaa
    34861 gcgccagtaa cagcaaagac atcggtcttg gatttgactg ccgtgtgctg tatctgcgtg
    34921 cgcagtgtgc catcaacatc gaatcaccaa acgacaagct gaacagcaac cttaatctgg
    34981 tggcgcgtga actggcgaag gttcgcgata aaggtctgcc ggaagaagag ttcaatgcgt
    35041 tagtggcgca aaagaaactg gagctgcaga aactgtttgc cgcctatgca cgggctgata
    35101 ccgatattct gatgggccag cggatgcgtt cgttgcaaaa tcaggtcgtc gatatcgcgc
    35161 cggagcagta tcagaaactg cggcaggatt tccttaacag cctgactgtg gagatgttaa
    35221 atcaggatct gcgtcagcag ttgtcgaatg atatggcgtt aatactgctg cagccgaaag
    35281 gcgagccgga atttaacatg aaagcgttgc aggcggcctg ggatcaaatc atggccccat
    35341 ctaccgccgc tgcgaccacc tctgtcgcca cggatgacgt acatcctgaa gtgacggata
    35401 ttccacctgc acagtaatgc ctgatggtga tgagttagca caaccatcag gtatttatgg
    35461 atgagctgga tagaggggta actctttatc cagccttttg catatgctgc gtttacgctg
    35521 gcatcgcctc acgcgggata atcgcgccgc gatactgaat aacggtactt gcggtcaggt
    35581 gcccgcgttt cgctgcgttt tccgcactgc cgcccgtcaa acgtaccgcc agataaccgg
    35641 cactgaaaga gtcgccagct gcggtggtat cgatcacttt ttctttcggc agtttcaccg
    35701 ccggaacatc cactaaccct tcgccagcaa tggacaccag gcaagaatcc gccccgcgtt
    35761 tcaccaccac ttctttcacg cccgcgttat gggtgcgcgc aatgacgtct tccaccggct
    35821 gttgacccca cagcgcgtct tcatcgtcca gcgtcaggaa ggcgatatcc gtgcattcca
    35881 gcatttgttg gtacacctgc tgtgtctctt ctttgctggc ccacaggcgc ggacgatagt
    35941 tattgtcgaa aatcactttt ccgccgttgg cgcggcattc gcgcagcagg gaaagcagct
    36001 tttcgcggct ggtcgggctt aagatcgcca ggctaatccc gctcaggtag agataatcga
    36061 aattcgccag ctcttcgcaa atcgccgcgg attgatcgct ctccagccag aatttggcgg
    36121 cggcttcgtt acgccagtag tagaacgtac gttcgccggt gctgtcggtt tcaatgtagt
    36181 aaaggcccgg cagacggttt tccatccgtt gggtcaggga agtatcaacg ttctcgccgt
    36241 gccaggcgtc cagcatctgc tggctaaaac tgtccgttcc cagcgccgtt acgtaatgaa
    36301 cggttaatgc cgcaggatcg acctgacggg cgatatagac ggaagtgttc agggtatcgc
    36361 cgccgaaacc gcgcttaacg tccgcgcctt tctcggaaag ctcaatcatg cattcgccaa
    36421 tcacggcaat ctttttggac atagtcgtga acctgatctg taaaaaatta gcgttagttt
    36481 gcgctgtggt ggtttgctgg tcaaccatat taaaacagtg ttccattatt tttttgagcc
    36541 agaacgtatt cctgaaagat tccgttgtgg agtggggaaa tttaccccgg ccaggcggta
    36601 aagttctgcc cttgcgcgcc gataatcttt gtcgagtccg ggctgcatca cttttaaaca
    36661 caggacatct ttgatgataa ggcaggttat ccagcgaata agcaaccctg aagcaagcat
    36721 cgagagcttg caggaacggc gtttttggtt gcagtgtgag cgtgcttaca cctggcagcc
    36781 gatctatcaa acatgcgggc ggttaatggc cgtggagcta ttaacggcgg tcacgcatcc
    36841 cttgaaccct tcgcaacgcc tgccgccgga tcgctatttt actgaaatca ccgtcagcca
    36901 tcggatggag gttgtgaaag agcagattga tttgctggcg caaaaagccg acttctttat
    36961 agagcacggc ctgctggcat cggtcaatat tgatggtcct acgctcatcg ccctgcgtca
    37021 gcaaccaaaa atcctgcgcc agattgagcg tcttccctgg ctgcgtttcg aactggtgga
    37081 gcatatccgt ctgccgaaag attcaacctt tgcctcgatg tgtgaatttg gcccgctgtg
    37141 gctggatgat tttggtaccg ggatggcaaa tttctctgca ctaagtgaag tgcgttatga
    37201 ctacatcaaa atcgcgcgag aactgtttgt gatgctgcgt cagtcgccgg aaggacgcac
    37261 actcttttct cagcttttac atctaatgaa tcgctattgt cgcggggtga ttgtcgaggg
    37321 cgtagaaacg ccggaagagt ggcgtgatgt acagaactcg cccgcattcg ccgcacaagg
    37381 ctggtttctt tcacgcccgg caccgataga aacgctgaat acggcggttc tggcgctata
    37441 agctgcctca ttttccgcct ggctggacta tctttaggac tggcacagga agaatatgag
    37501 gaagcgaacg atgagcaagg caggcaaaat aaccgctgcg atttcagggg ctttcttgtt
    37561 gttgattgtc gtggcgatca ttttgattgc aacttttgac tggaatcgac tcaaaccgac
    37621 catcaaccag aaagtctctg cggagttgaa tcgtccgttc gctatccgtg gcgatctggg
    37681 cgtggtgtgg gagcggcaaa agcaagaaac tggctggcgc agctgggtgc cgtggcccca
    37741 tgtacacgcg gaagacatca ttcttggcaa tccaccggat attcccgaag tcacgatggt
    37801 gcatttgcca cgcgtagaag caacgctggc cccgctggcg ctgctgacca aaacggtctg
    37861 gctgccgtgg atcaagctcg aaaagcccga cgcgcgcctg attcgcctct ctgaaaagaa
    37921 caataactgg acgtttaatc tcgccaacga tgataacaaa gacgcgaatg caaagccgtc
    37981 ggcatggtcg tttcggctgg ataatattct tttcgatcaa gggcggatcg ccattgatga
    38041 caaagtaagc aaagcggatc tggagatttt tgttgatccc ttaggcaagc cgctgccgtt
    38101 cagcgaagtt actggatcga aaggtaaagc ggataaagaa aaggtgggcg attacgtttt
    38161 tggcctgaag gcgcagggac gttataacgg tgaaccgctc acgggtacgg gaaaaatagg
    38221 cggtatgctg gcgctgcgtg gcgaagggac gccgtttccg gtacaggctg atttccgttc
    38281 aggtaatacc cgtgttgctt ttgatggcgt cgtgaatgac ccaatgaaga tgggcggtgt
    38341 cgatttacgg cttaaatttt ctggcgattc actgggtgat ctctatgaac tgacgggcgt
    38401 tctgctgccc gataccccgc cgtttgaaac ggatggtcgg ctggtagcga aaatcgacac
    38461 tgaaaaatcg tcggtctttg attatcgcgg ctttaatggg cgaattggcg atagcgatat
    38521 ccacggttct ctggtctaca ccaccggcaa gccacgacca aaactggaag gtgatgtcga
    38581 gtcgcggcaa ttgcggctgg cggacctggg accgttgatt ggcgttgatt ccgggaaagg
    38641 ggcagaaaag tcgaaacggt ctgaacagaa gaagggcgaa aaaagcgttc agcctgcggg
    38701 caaagtgctg ccttatgacc gcttcgaaac cgataaatgg gacgttatgg atgccgatgt
    38761 tcgcttcaaa gggcggcgca ttgagcatgg cagtagcctg ccgattagcg atctttctac
    38821 tcatatcatc ctcaaaaatg ctgacttgcg cctgcaaccg ctgaaatttg gcattgcggg
    38881 tggcagcatt gcggcgaata ttcatctgga aggcgataaa aagccgatgc aggggcgggc
    38941 agatattcag gctcgtcgac tgaaactgaa agaactgatg cccgatgtgg aactgatgca
    39001 gaagacgctg ggggaaatga acggtgacgc ggaactgcgc ggtagcggta actcggtggc
    39061 ggcactttta ggcaacagta acggcaacct gaaactgttg atgaatgacg ggctggtgag
    39121 ccgcaacctg atggagattg ttgggctgaa tgtcggcaac tacattgtcg gtgcgatatt
    39181 tggtgacgat gaggtgcggg tgaactgcgc ggcggcgaat ctgaatattg ccaacggcgt
    39241 ggcacgcccg cagatttttg ctttcgatac tgagaacgcg ttgattaacg ttaccggcac
    39301 ggcaagtttt gcttcggaac agctggattt gactattgat ccggagagta aaggtattcg
    39361 gattatcaca ctgcgttcgc cgctgtatgt gcgtgggacg tttaaaaatc ctcaggctgg
    39421 ggtgaaagcc gggccgttga ttgcccgtgg tgctgttgct gcggcactgg caacgctggt
    39481 tacgccagcg gcagcgttgc tggcactgat ctcaccttcc gaaggggagg ctaatcagtg
    39541 ccggacgatt ttgtcgcaga tgaagaagtg aagataattg ccggatgtct gacatccggc
    39601 aattaaacag atttagattg ttggcaaagt gtgctttgtt catgccggat gcggcgtgaa
    39661 cgccttatcc ggcctacaaa tccttgcgaa ttcaatatat tgtatggaaa ttcaggcctg
    39721 ataagcgtag cgcatcaggc ttttcactct tattacaacg actgatgtcg cgtctcatgg
    39781 gtcagcagca gggcgattaa cgtcaagcca gccatcgccg ccagatataa ccccaccgca
    39841 cttagcccgt agttagtctg caaccaggct gcgatatatg gcgcaacgga agccccgaga
    39901 atcgacgcta cgttgtaaga gaacgatgct ccggtgtaac gcacttctgt cggaaacagc
    39961 tctggtaaca gcgcacccat tggcccgaag gtcagaccca tcagacttaa ccccagcagc
    40021 aggaaggcaa aaaccagaat cgggttgcca gaaccgagca gtgggttaaa ggcgaacagc
    40081 gcgaacagga tgatcagcgt ggtgatgatt accatgcttt tacggcgacc aaaggcatca
    40141 gccagtaatc cagcgactgg caccatcacg ccaaaaccaa taactgccat catcaacatc
    40201 cacaacactt cgttacgcgg caggccaagc ccaactggcg cggcggcggt actaaaggtc
    40261 atagagtaga ccgtcatgat gtaaaacagc gtataggttg ccagcataat gaacgtaccc
    40321 agtacggtta cgcgaacatg tttggtcagc agcgtaccca gcgggatctt cacctgtttt
    40381 ttcgctttag cgactttctc aaacaccggc gactcatgca gcgacacgcg aacatacagg
    40441 ccgataatga ccagcaccgc cgagaagata aatggcacac gccagcccca gctcataaac
    40501 tgctcgtcgg tcagtagcca ggaaagcagc aagaaagtgc cgttggcaaa gaagaagcca
    40561 atcggtgcgc ccagctgcgg gaaggagccg tacaatgcac gtttgcgcgg tggggcgttt
    40621 tcagtcgcca gcagcgccgc gccgccccat tcaccaccta agcccagacc ctgaccaaat
    40681 cgagccagcg ccagcagcag cggggcgaaa ataccaatcg tggcatagcc tggcagcaga
    40741 ccaatcacca cggtcgaaat ccccatcgtt agcaacgagg cgaccagcgt cgctttacgc
    40801 ccaacgcgat cgccaaaatg accaaaaacg gcagagccaa tggggcgcgc gacgaaggcg
    40861 atggcgaagg tggcgagcga ctgtagcgtt gctgccgtag gatcgccctg cgggaagaag
    40921 atatgcggaa acacaataac ggccgcagtg gcgtaaatgt aaaagtcgaa gaactcaatg
    40981 gccgtgccaa tgagagaggc gacaaggact ttattacgcg agttgatcgg cgtgtattct
    41041 tgctcgtggt cgagtgttgt ggctgttgct tgcataatct tttcttattt tttggctaac
    41101 gaatagccat attacgcaca gcaaaagtga tatttcaatg tgtaacaaat cgcggaaatg
    41161 gtcaaaaacc aggcaaaaag gggatgatgt ttaggccagt gatttttctt ctatgaaatg
    41221 cttcacataa ttttataaat agtggataaa actggtttca ggttaaataa aagttaccaa
    41281 ctggatttat atgctggggt tatgagttgg catgaaattg tttgctcctg aatagatggc
    41341 tgaagaggct ctgggaaaga gtttcaagag gttatgtgcg cattatagcc ggatgcgatg
    41401 ctggcgcatc ttattcggcc tacggggagc caggccgaat aaattcgtat gccggataag
    41461 gcgttttgaa ctgcatccgg cctattttaa ggctgcgttt tccccggcat tcgcgggtcg
    41521 tctttatatt cggcggtggc aatccacgcc gcgcaaaaca gcgtcaaacg ggcgaagaag
    41581 tagaaaaatg ccatcagccc cagcacggaa ccaaatgctg cgccagaggg ggatttcatc
    41641 aacgatggca gggtgtaggt catcacgatt ttaatcactt caaaaccaat agcagccaga
    41701 aatgttccgc gaatcagggc ttttttgcgc ggacggtggc gcggcaaccg ccagaagatc
    41761 cagaagaaaa gcagatagtt ggcgaagatg gaaatcgcca ggccaatcaa ccgccacgtc
    41821 ggtttcagcc actcaatgct gttcaggtgc agggcgctaa taatcatttg ctgcgccgaa
    41881 ccggcaaccg aggtgatcga aagcgtcaca atcagcgcaa tcaacaaacc aatcagcgaa
    41941 ataaaatcac gcagatattt tacccagaac ttctcctgat cttgcggcga gcgttcccag
    42001 acatcgcgcg actgggcacg aatcgcttca cgcaggttac ccatccagtt gatgccggaa
    42061 taaagcgcca ccgccaggcc gacaagccct acagtcgtac gctgctgaac ggcggtgttg
    42121 atggtgtttt tcaacgtagc ggctagcgtc ggatcgctga tgttttgcag aattttgtcg
    42181 aagatatcct gtagcaacat cggatgggag gccagcacaa agccccctgc ggcaaacgac
    42241 accatcaaaa tcgggatcat cgacaaaaat gagaaatagg tgatagccgc accaaactgg
    42301 ttacccagcc gatcgttaaa gcgttctgtc gcgcgaatca ggtgcgcaat aaccggttgg
    42361 cgttggactt tttcggcagt ggtggtgacg gtttccagcg cctggctaac tttgccgcct
    42421 ttctcgctat tatccaactg cttaatcggc tcgtgctcca gatcctgggt gggacgtttg
    42481 atctcgtttt cctgcgtcat ccgctctttt gtccttctgt cgtggtcatt cttaaaaagt
    42541 atagtcggtc agtccaggta ctctttcatt acgccagcca gccactgcat aaacaggttt
    42601 acacgccggg aaagctcccg acgctgcgga taaaccagcg aaagggagag cggctcggca
    42661 cggtagcctg gcaacacttc aataagccgc ccggcacgca gggcttcgcg cacggcaatg
    42721 cgcgggatct gaataatccc cagaccggca agaccggcgg cgagatagtt ttcgctactg
    42781 tttaccgtca acatgccgcc agacttaaac cactggacgc cattaacgct ggcaacctca
    42841 aaacctaacg gatgtacacc caggtgcggt gtataacgca ctattgcgtg tgaagtgaga
    42901 tcgtcggggt tttgcggata accaaagcgc gtcagatagt gcggactggc acagttgacc
    42961 atggtcagtt tgccgagggg acgggcgata acgccatctt ccggtaacgc gccagtgcgt
    43021 atcacgcaat caaaaccatc atgaagaata tctaccggac ggtcatggct actcagttcc
    43081 agctcaattc ccggatgctg atagagaaat tccgacaggt gcggcagtaa caggcttttt
    43141 gcgattcctg gcgggatgtc gatgcgtaat ttaccgctga tactggtggc atcctgctga
    43201 aacagaccgt ccagttcgtt gagattactc aacacatctt ttgctcgttg ataataggtc
    43261 atgccttctg gcgtgagttt gacccgtcgc gtggtgcgct ggagaagctg ggtgccaagt
    43321 tgatgttcca gtgcctgtat ctggcgcgaa acacttcctt ttggcaaagc aaagaaatcc
    43381 gctgcgcggg aaaaactttc cagctccgcg actttgatga acaactgcat tgcgtgaatt
    43441 ttatccattt ctttgaccga ttgttgttca cagcgaaaca gtaacgcgca tatcgcattg
    43501 tttattattt tttctaacat ctaatgccgg gtttcacaat gttgtcatga cgaatgtggc
    43561 gatttctcag caggaaaagg gcggaatagc tttctgagaa aggtgaatcc ccttaaacag
    43621 agcgtatttt tcagcgattc cctttaaagt gaatagagga catattggct atttgtgctt
    43681 atttttgtct cattgccatt tgttattttg gcttgtggtc gcggaatttc atttttaatt
    43741 gcatgcgtac taaccaccaa tataaggaaa tcctcaaaat tgttttaaat agtgccctcg
    43801 acatgtaatt tcaggcgttt agctgtaact atttatatcg gtaactattt tctacgcgcc
    43861 atctctgtag aatggccacc gccaaatggc gaattcttat tttttctcgc gggtaatacc
    43921 cttccactac ttaagtcttt ttaacgtatt caggtggaga ttcagttatg caaatagtca
    43981 tgtttgacag gcagtcaata tttattcatg gaatgaaaat cagtttacag cagcgtattc
    44041 caggagtgag tattcagggg gccagtcagg cagacgagtt atggcaaaag ctggaaagtt
    44101 accctgaagc cttagttatg ctcgatggcg atcaggatgg tgagttttgc tactggttgc
    44161 tgcaaaaaac cgtggtgcaa tttcctgagg ttaaggtgtt aattacggcg acggactgca
    44221 ataaacggtg gttacaggaa gttatccatt ttaatgtgct ggccattgtg cctcgtgatt
    44281 caaccgtcga gacatttgcg ttggcggtaa atagtgcggc gatggggata atgtttctgc
    44341 ctggagactg gcggacgacg ccggaaaagg acattaaaga tctcaaatcg ttaagcgccc
    44401 gccagcggga gattttaacc atgttagcgg cgggcgaatc aaataaggag atcggcagag
    44461 cgctgaatat cagtaccgga acggtaaaag cgcatctgga atccctgtat cgccgtctgg
    44521 aagtgaaaaa tcgcacccag gcggcaatga tgttaaatat ctcctcctga agaggataat
    44581 tttacagcca gcaggaagtt agcggctggc tgtaaaaata ttatggttcg ccgtacaaac
    44641 caattaaacg gcgtaccaca ccgttagtcc agccaaaccc atcctgcaac ggatactcgc
    44701 caccgccgcc ttcgcgggga acaccatcgg caatatggta tttttcgatc agtttgtgct
    44761 gttccagata aaactgattc accgtcttca gccagctgcg cgcgatttca tcgcccagaa
    44821 ggtcatcgcc atacatttta aatccctgaa ttgccatcca ttgtaacggt gcccagccgt
    44881 tgggtttatc ccactgttca ccggtttcgt actcgcttgc cagaatcccg ccaggtgtca
    44941 gtaaccgact gcgcacggcg tttgccagac gatcggcctg ttcatggttc gccataccga
    45001 catagagcgg cacaatggcg gcagcggaaa acagcgccag ttgttcgcgt cgccagtcgt
    45061 aatcgcggta gatgccgttt tcatcatccc agaggtaacg gtttaccgca tcgcgacggg
    45121 cactggcctt ctggcggaac agtgcttctg tctctttctc acctttcagc gccgagatgt
    45181 tggcgatggc gctctccagt ttaaacagga aggcattcag atcgatgggg atgaactggg
    45241 tggtacgaat gctcgccaga cgaccagtat cacggagcca acgggaagag taatcccaac
    45301 cggaggccgc ccccgcgcgt aaatcgcggt acacctcgtt gggcgggcga ccagaatgtt
    45361 tcgcggtttc aacgtcctca agccaggatt cgtcacgcgg cgtgtcgcga tcgtcccagt
    45421 aacggttgag cagcgatccg tccggcatcc gcacaacatg gcgataggcc tgattaggga
    45481 tcaacgattc tgcaccgtcc atccagaagg catattccat tttcaggtgg tcgagatagc
    45541 ggcgcgcacc gcgtacacca tcttcttcaa acaactccac catcagcgca aaaaccggtg
    45601 gttgcgagcg gctcaaataa taggtgcggt tgccgttggg gatgtgaccg tagttttcga
    45661 tcatccaggc gaagttatcg gccatgcatt tcagcaaatc ttcccgacca ctttccgcca
    45721 gccccagcat ggtgaaatag gaatcccaat agtaggtttc gctaaaacgg ccgcccggga
    45781 caatatatga ctgcggcagc gccagcagag aagaccacgg aatgtgatcc tgtggttcgc
    45841 gggttagcac cggccacagc tggtcgatat gctctttcag ggaattttgc gggtccgata
    45901 catactcgct ggagtagacc tccggcagcc agaagtgatt ttcaacaaac ttgcgcaagt
    45961 caaaatcacg atgacggcgc actttacggt agcggattaa aatatccagc gggtccattt
    46021 tgggtgcgca gtcgggaaag gttttactgt cggggaaaat tttcgccgac tgaacatgct
    46081 cgaacagttc gagatagcga tcggcaggcg tcagcgcatc agaggcgggc agcccttcaa
    46141 tcatctcggg ttccggttct gcctcgatca tctcatccag ttttaattca tacgggtcca
    46201 gttcatagca gagatcgacc tcgatcatca gttcgtctgg attagggttt tgaattttct
    46261 gattgagcat aaggatccaa cctccgaaat tcgtcgctaa ttatcgggct tttctttaag
    46321 ggtagtcatt cgctccgcct ggtgggcgac aaagcgtgcg gtggatcacg tttattaacc
    46381 tgtcaaaaaa tataacgcac taatatcaat ggaatttata aaaatacaga tttcgtgttg
    46441 tttttcattt tcagaacatt caatcctgct cttgccgcaa tcatgatatg ccattcctga
    46501 tctgggtaat aggcacaggc tatcttattg atagtttata ttcatgtaat tgattgattc
    46561 ctatcaaaaa gcgtatctcc ctgtttttta tgatggcttt gtctcgcaga gacgtttgca
    46621 gcattctgga aaattttctg ggtcataata actcaccacc gtgagggaag aataatgaaa
    46681 atggtctcac gtattaccgc gatcggcctg gctggcgtcg cgatttgcta tttagggtta
    46741 tctggttatg tgtggtacca cgataataaa cgcagtaaac aggccgatgt tcaggcatct
    46801 gctgtcagtg aaaataataa ggttttaggc tttctccgcg aaaaaggatg cgactattgc
    46861 cacacgcctt cggcagaatt acccgcctat tattatattc ctggcgcgaa acagttgatg
    46921 gattacgaca ttaagcttgg atataaatct tttaacctcg aggccgtgcg tgctgctctg
    46981 ctggctgata aacccgtttc gcaaagcgat ctgaataaga ttgaatgggt gatgcagtat
    47041 gaaactatgc cgccaacgcg ttataccgcg ctacactggg cgggtaaggt gagtgatgaa
    47101 gagcgggcgg aaatactggc ctggattgca aaacagcgcg cggaatatta cgccagcaat
    47161 gatactgctc cggagcatcg caatgaaccg gtgcagccca tcccgcaaaa actgcctacc
    47221 gatgcgcaaa aagtggcgtt gggctttgcg ctgtatcacg atccccgttt atcggctgat
    47281 agcaccattt catgcgctca ttgccatgcg ttgaatgcgg ggggcgtcga tggcagaaaa
    47341 acatcgattg gtgttggtgg cgcagttggg ccgattaacg cgccgacggt atttaactca
    47401 gtatttaacg ttgagcagtt ctgggatggt cgtgcggcaa cattgcagga tcaggctggt
    47461 ggaccgccgt tgaacccgat tgaaatggcg tcaaaatcct gggacgaaat tattgctaag
    47521 ctggaaaaag atccgcagct taaagcgcag ttcctcgacg tctatccgca aggtttcagt
    47581 ggcgaaaata ttactgatgc cattgctgaa tttgagaaaa cattaattac gccggattcc
    47641 ccatttgata aatggttgcg tggggatgaa aatgcgctga cggcgcaaca gaaaaaaggc
    47701 tatcaattat ttaaagataa taaatgtgca acttgtcatg gtggaattat tctcggcgga
    47761 cgttccttcg aaccgttggg gctgaaaaaa gactttaact ttggggaaat tacggcggcg
    47821 gatattggtc gtatgaatgt gactaaagaa gagcgtgata aattgcgtca gaaagtaccc
    47881 ggtttacgta acgttgcttt aacggcaccg tacttccatc gcggtgacgt gccgacgctg
    47941 gacggggcgg tgaaactgat gttgcgctat caggtaggca aagagctgcc gcaggaggat
    48001 gtggatgata tcgtagcttt cctgcacagt ctgaacgggg tttacacgcc gtatatgcag
    48061 gataaacaat aattaatttg atcgcccgaa caggaatgtt tgggcgattt ttattacgat
    48121 aataaagtct gtttttaata ttatcatatt aaatgtttat attataaaag atcatttttc
    48181 tgcttaggat tttgttattt aaattaagcc tgtaatgcct tgcttccatt gcggataaat
    48241 cctacttttt tattgccttc aaataaattt aaggagttcg aaatggacca gaagctgtta
    48301 acggatttcc gctcagaact actcgattca cgttttggcg caaaggccat ttctactatc
    48361 gcggagtcaa aacgatttcc gctgcacgaa atgcgcgatg atgtcgcatt ccagattatc
    48421 aatgatgaat tatatcttga tggcaacgct cgtcagaacc tggccacttt ctgccagacc
    48481 tgggacgacg aaaacgtcca taaattgatg gatttgtcga tcaataaaaa ctggatcgac
    48541 aaagaagaat atccgcaatc cgcagccatc gacctgcgtt gcgtaaacat ggttgccgat
    48601 ctgtggcatg cgcctgcgcc gaaaaatggt caggccgttg gcaccaacac cattggttct
    48661 tccgaggctt gtatgcttgg cgggatggcg atgaaatggc gttggcgcaa gcgtatggaa
    48721 gctgcaggca aaccaacgga taaaccaaac ctggtgtgcg gtccggtgca aatctgctgg
    48781 cataaattcg ctcgctactg ggatgtggag ctgcgcgaga tccctatgcg ccccggtcag
    48841 ttgtttatgg acccgaaacg catgattgaa gcctgcgacg aaaacaccat cggcgtggtg
    48901 ccgactttcg gcgtgaccta caccggtaac tatgagttcc cgcaaccgct gcacgatgca
    48961 ctggataaat tccaggccga caccggtatc gacatcgaca tgcacatcga cgctgccagc
    49021 ggtggcttcc tggcaccgtt cgtcgccccg gatatcgtct gggacttccg cctgccgcgt
    49081 gtgaaatcga tcagtgcttc aggccataaa ttcggtctgg ctccgctggg ctgcggctgg
    49141 gtgatctggc gtgatgaaga agcgctgccg caggaactgg tgttcaacgt tgactacctc
    49201 ggtggtcaga ttggtacttt cgccatcaac ttctcccgcc cggcgggtca ggtgattgcg
    49261 cagtattatg aattcctgcg cctcggtcgt gaaggctata ccaaagtaca gaacgcctct
    49321 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
    49381 tgtacgggtc gtccggacga aggcatcccg gcggtttgct tcaaactgaa agagggtgaa
    49441 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
    49501 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
    49561 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
    49621 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacacacc
    49681 tgataacata acgttgtaaa aaccgaatgc ccagccttta aaaaaaacag ctgggcattc
    49741 ggttgcttat taatcgcaat aatatattgg ctgtctattc atcgtgttga taagatatat
    49801 gttatatgaa tgtttattaa atgtagcgat tacctttaat gtcacgacaa gggattattt
    49861 gcttactatt aatttccctg cgttcttaaa cacgtttttg tattataaat ccagtcatcc
    49921 tgcccgtaga acatcacaca ttatcatcct gttctcccgc ttcgtttaaa tttatttatc
    49981 aatcaatttg acttaagagg gcggcgtgct acattaataa acagtaatat gtttatgtaa
    50041 tattaagtca actatgcaat cattacatgg gaattgtcta attgcgtacg caagacataa
    50101 atatattctc accatggtta atggtgaata tcgctatttt aatggcggtg acctggtttt
    50161 tgcggatgca agccaaattc gagtagataa gtgtgttgaa aattttgtat tagtatcaag
    50221 ggatacgctt tcattatttc tcccgatgct caaggaggag gcattaaatc ttcatgcaca
    50281 taaaaaagtt tcttcattac tcgttcatca ctgtagcaga gatattcctg tttttcagga
    50341 agttgcgcaa ctatcgcaga ataagaatct tcgctatgca gaaatgctac gtaaaagagc
    50401 attaatcttt gcgttgttat ctgtttttct tgaggatgat cactttatac cgctgcttct
    50461 gaacgtttta caaccgaaca tgcgaacacg agtttgtacg gttatcaata ataatatcgc
    50521 tcatgagtgg acactagccc gaatcgccag cgagctgttg atgagtccaa gtctgttaaa
    50581 gaaaaaatta cgcgaagaag agacatcata ttcacagttg cttactgagt gtagaatgca
    50641 acgtgctttg caacttattg ttatacatgg tttttcaatt aagcgagtcg cagtgtcctg
    50701 tggatatcac agcgtgtcgt atttcattta cgtctttcga aattattatg ggatgacgcc
    50761 cacagagtat caggagcgat cggcgcaggg attgccgaac cgtgactcgg cggcaagtat
    50821 tgttgcgcaa gggaattttt acggcactga ctgttctgcg gaaggaataa gattatagag
    50881 ttttactcag acataaaaaa aacccggcat aggggaccgg gaagaggata gtctgccgtc
    50941 tccagactaa taaaccgtta taacactccc tgttggcacg ggaaactttg tgctctcagt
    51001 aagttaaata taacttttac tggaaataag atcagccatt tttttataaa cataagctat
    51061 acgctgtgcg aaaatatatt cgtgttgcat ttacttatta tcaattaact gttatgcaaa
    51121 actactttgt ggataaattt tggtcctacc aaatttggca gtttttgcgc taagaaacag
    51181 tctggcatca tttcattagt atactgaaat taaaataatc gcagtatgaa atataaggga
    51241 taatcatgac tcatgtctgc tcggtgatcc tcattcgtcg ttcattcgat atttatcatg
    51301 aacagcaaaa aatatcgctg cataacgaga gtatcctgct gctggagaaa aatttggcag
    51361 acgattttgc gttttgttca ccggatacgc gacgactgga tatcgatgag ctgacagttt
    51421 gccattactt acaaaatatt cgtcagctac cacgcaattt agggttacat agcaaagacc
    51481 gtctgttaat taaccagtca ccccccatgc cgctggtgac ggcgattttt gatagcttca
    51541 atgaatccgg ggtaaattca ccgatactga gcaatatgct ctacctttcc tgtttatcga
    51601 tgttttctca taagaaagaa ctgatcccct tacttttcaa tagcatcagc actgtttcag
    51661 gaaaagttga acgccttatt agctttgata tcgccaaacg ttggtatctg cgcgatatcg
    51721 cggaaagaat gtataccagc gagagtctca tcaaaaaaaa gttgcaggat gaaaacacct
    51781 gtttcagtaa aatattactc gcctccagga tgtcgatggc cagacgatta ctcgagttac
    51841 gtcaaattcc tctgcatact attgcggaaa aatgtggcta tagcagtacg tcgtacttta
    51901 taaacacatt tcgacaatat tatggtgtaa cgccacatca gtttgcgcaa cattcgccag
    51961 gtaccttttc ctgacatatt ttgcatttga atattggtca ggatctcaca cctgcttcat
    52021 gtgaaactct tccctgatga tttctgccgg gctaccggct agttctcttt cgcagtttgc
    52081 caggcggtat aggtattttc aggaggaaaa gactcggcat gtttgggatt attaagctga
    52141 caattcatac gattacggga atgtgggtca gtattgtgct gttcaaattg atgacgaatg
    52201 gctggagtgg tttctacttt cagtgctgtg tactaagcct ggtattcctg acggttagct
    52261 ggttgttgag tggtgaatgg ttagcaggaa agagtaaggc tgaaccttca cgttcaacct
    52321 tactctcatt tacacgttac gcttttttaa agcgggcaaa gagatgttcc actacaacga
    52381 aaaagaccgg aacaaagtaa attgccagca ctgttgcggc aaacatcccg cccatcacgc
    52441 cggtacctac cgcgttttgc gcgccagaac cggcaccatg actgataacc agcggcagca
    52501 cgccgagaat aaaggccaga gaggtcatca ggattgggcg taaacgcatc cgcgccgctt
    52561 cgatgattgc ctctatcggc gttttccctt ctttctgcat catctcaacg gcaaattcga
    52621 caatcaggat ggcgttcttg gcggaaagcc cgatggtggt cagcaaacca acctggaagt
    52681 agacgtcatt acttaagccg cgcagatcgg tggccagtaa tgcgccaacg acgcctaacg
    52741 gaacaaccaa catcaccgag aacggaattg accagctctc ataaagtgcg gcgagggcga
    52801 ggaacaccac gaccagtgaa atcgcataca gcgcaggagc ctgatttgag gataacgctt
    52861 cctgatacga cagtccggtc catgagtagc cgacgcctgc cggaagttta gcgaccaggt
    52921 ctgccataaa tttcatggcg tcaccggtac ttttcccggc cgccgcttca cctaaaatct
    52981 ccattgacgg gatgccgttg tagcgttcca gtcgcggtga accataggtc cattcggtag
    53041 acgagtaggc agaaagcggt gccatcgtgc cagaggcgtt gcgtacatac cattggttga
    53101 tgttatccgg caacatacgg aacggcgtgc ctgcctggac atacactttt ttcacccgcc
    53161 cctggttgag gaagtcgttc acgtagctgc tgccgaaggc ggtggaaatt gtctggttga
    53221 tatcagacag cgccacaccc attgcctcag ctttcgcggc gttgacgttc actttgaaca
    53281 tcggcgtatc ttccaggccg ttcgggcgta ccccggtgac ctgattcggt gattgcgctg
    53341 ccagtgataa cagctcgttt cgtgcctggg ttagtttttc gtgccccagg ttaccgttgt
    53401 ccagcagttc catatcaaaa cctgacgcgg tacccagttc agccaccgcg ggtaagttga
    53461 acgggaagac gacggcttta ttgatactgc ttagcgcaat cattgcccgc tgaatgatcg
    53521 cggtaaccga gttttcctca ccgacacgtt cagaccacgg cttgagactg ataaacgcca
    53581 gaccgttgtt ttgcccctgg ccgctgaagc caaagccgcc aacggtaaac accgactgaa
    53641 cattatcttt ctcttttgta agataataat ccgtcacctg ttgcagcact ttcgtggtgt
    53701 taaccatggt ggcaccggaa ggtaactgcg cggtggtcat aaataccccc tgatcctctt
    53761 ctggtaagaa agaggtcggc gtgcgcagga acagcaccgc catcccggcg caaatcagca
    53821 ggtagatcac catgtagcga ccggtacaac gcaacagtga gcgggtgcta tcggtatagt
    53881 gctgagttga tttttcaaac agcgtgttga agcgtgcgaa cagggcatta ggtttgtgac
    53941 cgccttccgg cgcggctttc agaatggtgg cgcacagggc aggggtcagg ctcattgcca
    54001 caaatactga aagcagcatg gaggagatca gcgtgatgga gaactggcgg tagatctccc
    54061 cggttgcacc gctcataaag gccatcggca taaacactgc ggaaagaaca acggcaatac
    54121 cgaccagcgc acgttggatc tgccccatcg atttatgcgt cgcttccttc ggtggtagct
    54181 tatcttccgc aatgacacgc tcgacgttct ccaccaccac gatggcgtca tccaccagta
    54241 accctatcgc cagcaccatc ccgaacatag tcaacgtgtt gatggtgaaa ccgaccgccg
    54301 acaagatcgc aaacgtcccg agaataacca ccggtacggc aatcgtcggg atgattgtgg
    54361 cacggaaatt ctgcaaaaac agatacatga ccaggaagac gaggatgata gcctcaacca
    54421 gtgttttgaa aacttcctga atagaaattt cgataaacgg cgtggtgtcg taaggataaa
    54481 ccgtcttcag acttgccggg aaataggctg ataagcggtt cagttcctct ttgactgccc
    54541 gcgaggtatc cagggcgttt gctccggtag ccagtttgat ggcgatcccg gcagcaggtt
    54601 tgccgttata gcgcgccacg gtggaataat cttccgcccc aagttcgacg cgggcgacat
    54661 cacgcagcag cacttgcgaa ccatcttgct gaactttcaa caggattttg ccaaattctt
    54721 ccggcgtttg cagacgcgtc tgcacaatga tcgaggcgtt tagttgctgg tctgccgcct
    54781 gtggcatgcc acccagttga ccaccagaaa tctggttgtt ttgcacctta atctgggaaa
    54841 taacatcgga aggtaccaga ttgtatttat tgagtttttg cgggtccagc cagatacgca
    54901 tggcgtactc ggaaccaaag agctgtacgc taccaacgcc cgcggtacgg cttagcgggt
    54961 ctttgatatt agacgctaca tagtccgcga tatcgtactg gttgaggctg ccgttatcag
    55021 aaataaacgc cgctaccatc aggatattac tgctcgattt atcgacgcta atcccctgct
    55081 gctgcactgc ttcaggtaat gaaggcatag cgagttgcag tttattttgc acctgaacct
    55141 gtgcgatatc aggagatgtc ccagtctcga aggtcagagt gatagaggca ttacccgccg
    55201 catcactggt tgaagacatg tacatcaggc catcaagccc attcatattt tgctcaatca
    55261 cctgagtgac cgagtcttct accgtttgcg catcggcacc cggataggta gcgctgacgg
    55321 taatggtcgg tggcgcaatc tgcggatact gcgcaaccgg taagttcatg atcgccagac
    55381 cacctgcaag catcataata atggcaagta cccaggcaaa aaccgggcga tcaataaaat
    55441 agttagccat gaaagtcccc ttaagcctgc aacgttattg tttcgattcg gtgctggcgt
    55501 tttcctggct ggaggaaatt gctcgtgctt tgatacccgg acgaatgcgt tgcaaaccgg
    55561 aaacgatcac ccgatcgcca gcctgcaagc cagaggtgac aacccactgg tcgccgatgg
    55621 ctttgctggc ttcaatttcg cgtagcttca cgacatcgtc tttatccaga atgagcgccg
    55681 ttgctttacc ttgggcgttg tgggtgacgc cttcctgcgg caccagtaat acattctggc
    55741 ggctaccttc atccactaat gccgtgacat acatgccagg cagcaagtca ccatttgggt
    55801 tggggaaaat cgcccgtaac gtcacggagc ccgtggtttc atcaaccgtc gggtcggaga
    55861 atttcagcgt gccggtctgg ctgtaacgtt taccattttc cagattgagc tgtactggcg
    55921 tactgccctg aacctgtttg atttgcccac tggcgacctc ttctttcatg cgtaagaaat
    55981 cttgcaccga ctgcgtgaga tcgacataaa tcgggtccag acgttgtacg gtaaccagcg
    56041 aatctgcctg attagcggta acgagtgcgc cgacggtcac cgacgatttc ccgctgacgc
    56101 ccgtaatcgg cgaggtgaca ttcgcgtatt gcagattgat cgtcgcctgt tcaacagccg
    56161 ctttggcgac ggtgacattg gcttctgctt cattcaactg ggtgcgcgcg gtgtcgtaat
    56221 cctgacggct aacgtagttg gtcttcagca acgatgcctg gcggttaaag gtgatgcggg
    56281 cattgctggc ggtagagagc gctttcgcca gcgagccttt ggcggagttt agctcggcct
    56341 gtaaaggtgc aggatcaatc tgatacagcg aatcgccctg gttcacttta tcgccttcga
    56401 taaagttgcg tttaatgata ataccgccca cctggggacg tatctcggca acttcataag
    56461 gaacggttct accgggcaat tcgctcaaca cattgaccga cccaggggag agtgtgacga
    56521 caccgacctc aggcgtcatg gcggcggtgt tttccgccga tttgtcatcg caggcggtga
    56581 gcatcgcgcc gcagaataac aacggtatta acagctttct tcttctgttc attttagtcc
    56641 ctgaaaattc ttgagaaata acggttcttt aaattgcgcg cgaggagaga tatgagggat
    56701 ttacagacca gcggcatagt ggggttgtca gccagtaagg tgggatacag gcatagtgat
    56761 cgacatggtg aggtcaacga caaaaaaaat gttgccgttc tgccaacagt tcctgccagc
    56821 attcgggcaa cgaagggttc tactggtgga tacacatacc aggggaataa agtcgtttta
    56881 gcctctcctt tagtaatcac cgggggtaat gaaatcagca tctgcatccc tcgtcatgcc
    56941 agccatcaat ttcagttgct tatgtcctga ctaaaaataa gatgtgatac ccagggtgac
    57001 gatgtcgctc atacgtctta gtttcagctt gtacataatg ttgaatttct gaatctttaa
    57061 tgtttgttgc gaaatattga gcgtcgacgt gatattgctt tcatttttat ttttcaga
//
LOCUS       DAZFBR010000017        56315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFBR010000017 DAZFBR010000000
VERSION     DAZFBR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.017"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(357..443)
                     /locus_tag="ACHJZ2_001194"
     tRNA            complement(357..443)
                     /locus_tag="ACHJZ2_001194"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(407..409),aa:Leu,seq:gag)
     gene            complement(458..790)
                     /gene="secG"
                     /locus_tag="ACHJZ2_001195"
     CDS             complement(458..790)
                     /gene="secG"
                     /locus_tag="ACHJZ2_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO4567349.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1018..2355)
                     /gene="glmM"
                     /locus_tag="ACHJZ2_001196"
     CDS             complement(1018..2355)
                     /gene="glmM"
                     /locus_tag="ACHJZ2_001196"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO4567350.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2348..3196)
                     /gene="folP"
                     /locus_tag="ACHJZ2_001197"
     CDS             complement(2348..3196)
                     /gene="folP"
                     /locus_tag="ACHJZ2_001197"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO4567351.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3286..5220)
                     /gene="ftsH"
                     /locus_tag="ACHJZ2_001198"
     CDS             complement(3286..5220)
                     /gene="ftsH"
                     /locus_tag="ACHJZ2_001198"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO4567352.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5320..5949)
                     /gene="rlmE"
                     /locus_tag="ACHJZ2_001199"
     CDS             complement(5320..5949)
                     /gene="rlmE"
                     /locus_tag="ACHJZ2_001199"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO4567353.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6075..6368
                     /gene="yhbY"
                     /locus_tag="ACHJZ2_001200"
     CDS             6075..6368
                     /gene="yhbY"
                     /locus_tag="ACHJZ2_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO4567354.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6524..7000)
                     /gene="greA"
                     /locus_tag="ACHJZ2_001201"
     CDS             complement(6524..7000)
                     /gene="greA"
                     /locus_tag="ACHJZ2_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO4567355.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7248..8681
                     /gene="dacB"
                     /locus_tag="ACHJZ2_001202"
     CDS             7248..8681
                     /gene="dacB"
                     /locus_tag="ACHJZ2_001202"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4567356.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8721..9893)
                     /gene="cgtA"
                     /locus_tag="ACHJZ2_001203"
     CDS             complement(8721..9893)
                     /gene="cgtA"
                     /locus_tag="ACHJZ2_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO4567357.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9909..10874)
                     /gene="yhbE"
                     /locus_tag="ACHJZ2_001204"
     CDS             complement(9909..10874)
                     /gene="yhbE"
                     /locus_tag="ACHJZ2_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4567358.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11001..11258)
                     /gene="rpmA"
                     /locus_tag="ACHJZ2_001205"
     CDS             complement(11001..11258)
                     /gene="rpmA"
                     /locus_tag="ACHJZ2_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO4567359.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11279..11590)
                     /gene="rplU"
                     /locus_tag="ACHJZ2_001206"
     CDS             complement(11279..11590)
                     /gene="rplU"
                     /locus_tag="ACHJZ2_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO4567360.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11849..12820
                     /gene="ispB"
                     /locus_tag="ACHJZ2_001207"
     CDS             11849..12820
                     /gene="ispB"
                     /locus_tag="ACHJZ2_001207"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO4567361.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13049..13327
                     /gene="sfsB"
                     /locus_tag="ACHJZ2_001208"
     CDS             13049..13327
                     /gene="sfsB"
                     /locus_tag="ACHJZ2_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO4567362.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13375..14634)
                     /gene="murA"
                     /locus_tag="ACHJZ2_001209"
     CDS             complement(13375..14634)
                     /gene="murA"
                     /locus_tag="ACHJZ2_001209"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO4567363.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14689..14943)
                     /gene="ibaG"
                     /locus_tag="ACHJZ2_001210"
     CDS             complement(14689..14943)
                     /gene="ibaG"
                     /locus_tag="ACHJZ2_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO4567364.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15103..15396)
                     /gene="mlaB"
                     /locus_tag="ACHJZ2_001211"
     CDS             complement(15103..15396)
                     /gene="mlaB"
                     /locus_tag="ACHJZ2_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO4567365.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15396..16031)
                     /gene="mlaC"
                     /locus_tag="ACHJZ2_001212"
     CDS             complement(15396..16031)
                     /gene="mlaC"
                     /locus_tag="ACHJZ2_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO4567366.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16050..16601)
                     /gene="mlaD"
                     /locus_tag="ACHJZ2_001213"
     CDS             complement(16050..16601)
                     /gene="mlaD"
                     /locus_tag="ACHJZ2_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO4567367.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16606..17388)
                     /gene="mlaE"
                     /locus_tag="ACHJZ2_001214"
     CDS             complement(16606..17388)
                     /gene="mlaE"
                     /locus_tag="ACHJZ2_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO4567368.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17396..18205)
                     /gene="mlaF"
                     /locus_tag="ACHJZ2_001215"
     CDS             complement(17396..18205)
                     /gene="mlaF"
                     /locus_tag="ACHJZ2_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO4567369.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18415..19392
                     /gene="yrbG"
                     /locus_tag="ACHJZ2_001216"
     CDS             18415..19392
                     /gene="yrbG"
                     /locus_tag="ACHJZ2_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO4567370.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19406..20392
                     /gene="kdsD"
                     /locus_tag="ACHJZ2_001217"
     CDS             19406..20392
                     /gene="kdsD"
                     /locus_tag="ACHJZ2_001217"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO4567371.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20413..20979
                     /gene="kdsC"
                     /locus_tag="ACHJZ2_001218"
     CDS             20413..20979
                     /gene="kdsC"
                     /locus_tag="ACHJZ2_001218"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO4567372.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            20976..21551
                     /gene="lptC"
                     /locus_tag="ACHJZ2_001219"
     CDS             20976..21551
                     /gene="lptC"
                     /locus_tag="ACHJZ2_001219"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO4567373.1"
                     /translation="MSKARRWVIIVLSLAFLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21520..22077
                     /gene="lptA"
                     /locus_tag="ACHJZ2_001220"
     CDS             21520..22077
                     /gene="lptA"
                     /locus_tag="ACHJZ2_001220"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO4567374.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22084..22809
                     /gene="lptB"
                     /locus_tag="ACHJZ2_001221"
     CDS             22084..22809
                     /gene="lptB"
                     /locus_tag="ACHJZ2_001221"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO4567375.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22857..24290
                     /gene="rpoN"
                     /locus_tag="ACHJZ2_001222"
     CDS             22857..24290
                     /gene="rpoN"
                     /locus_tag="ACHJZ2_001222"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO4567376.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVKQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24313..24600
                     /gene="hpf"
                     /locus_tag="ACHJZ2_001223"
     CDS             24313..24600
                     /gene="hpf"
                     /locus_tag="ACHJZ2_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO4567377.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24718..25209
                     /gene="ptsN"
                     /locus_tag="ACHJZ2_001224"
     CDS             24718..25209
                     /gene="ptsN"
                     /locus_tag="ACHJZ2_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO4567378.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            25255..26109
                     /gene="rapZ"
                     /locus_tag="ACHJZ2_001225"
     CDS             25255..26109
                     /gene="rapZ"
                     /locus_tag="ACHJZ2_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO4567379.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26106..26378
                     /gene="npr"
                     /locus_tag="ACHJZ2_001226"
     CDS             26106..26378
                     /gene="npr"
                     /locus_tag="ACHJZ2_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO4567380.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26592..27224
                     /gene="yrbL"
                     /locus_tag="ACHJZ2_001227"
     CDS             26592..27224
                     /gene="yrbL"
                     /locus_tag="ACHJZ2_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO4567381.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(27221..27949)
                     /gene="mtgA"
                     /locus_tag="ACHJZ2_001228"
     CDS             complement(27221..27949)
                     /gene="mtgA"
                     /locus_tag="ACHJZ2_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO4567382.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(27946..28599)
                     /gene="elbB"
                     /locus_tag="ACHJZ2_001229"
     CDS             complement(27946..28599)
                     /gene="elbB"
                     /locus_tag="ACHJZ2_001229"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO4567383.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28829..31165)
                     /gene="arcB"
                     /locus_tag="ACHJZ2_001230"
     CDS             complement(28829..31165)
                     /gene="arcB"
                     /locus_tag="ACHJZ2_001230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO4567384.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31261..32190)
                     /gene="yhcC"
                     /locus_tag="ACHJZ2_001231"
     CDS             complement(31261..32190)
                     /gene="yhcC"
                     /locus_tag="ACHJZ2_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO4567385.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            32864..37324
                     /gene="gltB"
                     /locus_tag="ACHJZ2_001232"
     CDS             32864..37324
                     /gene="gltB"
                     /locus_tag="ACHJZ2_001232"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO4567386.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37337..38755
                     /gene="gltD"
                     /locus_tag="ACHJZ2_001233"
     CDS             37337..38755
                     /gene="gltD"
                     /locus_tag="ACHJZ2_001233"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO4567387.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            38939..39986
                     /gene="yhcG"
                     /locus_tag="ACHJZ2_001234"
                     /pseudo
     CDS             38939..39986
                     /gene="yhcG"
                     /locus_tag="ACHJZ2_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(40046..40510)
                     /gene="nanQ"
                     /locus_tag="ACHJZ2_001235"
     CDS             complement(40046..40510)
                     /gene="nanQ"
                     /locus_tag="ACHJZ2_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO4567388.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(40507..41382)
                     /gene="nanK"
                     /locus_tag="ACHJZ2_001236"
     CDS             complement(40507..41382)
                     /gene="nanK"
                     /locus_tag="ACHJZ2_001236"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO4567389.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(41379..42068)
                     /gene="nanE"
                     /locus_tag="ACHJZ2_001237"
     CDS             complement(41379..42068)
                     /gene="nanE"
                     /locus_tag="ACHJZ2_001237"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO4567390.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(42116..43606)
                     /gene="nanT"
                     /locus_tag="ACHJZ2_001238"
     CDS             complement(42116..43606)
                     /gene="nanT"
                     /locus_tag="ACHJZ2_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO4567391.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(43715..44608)
                     /gene="nanA"
                     /locus_tag="ACHJZ2_001239"
     CDS             complement(43715..44608)
                     /gene="nanA"
                     /locus_tag="ACHJZ2_001239"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO4567392.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVSEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(44730..45521)
                     /gene="nanR"
                     /locus_tag="ACHJZ2_001240"
     CDS             complement(44730..45521)
                     /gene="nanR"
                     /locus_tag="ACHJZ2_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO4567393.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            45901..47257
                     /gene="dcuC"
                     /locus_tag="ACHJZ2_001241"
                     /pseudo
     CDS             45901..47257
                     /gene="dcuC"
                     /locus_tag="ACHJZ2_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(47300..47797)
                     /gene="sspB"
                     /locus_tag="ACHJZ2_001242"
     CDS             complement(47300..47797)
                     /gene="sspB"
                     /locus_tag="ACHJZ2_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO4567394.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(47803..48441)
                     /gene="sspA"
                     /locus_tag="ACHJZ2_001243"
     CDS             complement(47803..48441)
                     /gene="sspA"
                     /locus_tag="ACHJZ2_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO4567395.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(48836..49228)
                     /gene="rpsI"
                     /locus_tag="ACHJZ2_001244"
     CDS             complement(48836..49228)
                     /gene="rpsI"
                     /locus_tag="ACHJZ2_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO4567396.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(49244..49672)
                     /gene="rplM"
                     /locus_tag="ACHJZ2_001245"
     CDS             complement(49244..49672)
                     /gene="rplM"
                     /locus_tag="ACHJZ2_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO4567397.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(49891..51018)
                     /gene="zapE"
                     /locus_tag="ACHJZ2_001246"
     CDS             complement(49891..51018)
                     /gene="zapE"
                     /locus_tag="ACHJZ2_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO4567398.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            51212..51610
                     /gene="zapG"
                     /locus_tag="ACHJZ2_001247"
     CDS             51212..51610
                     /gene="zapG"
                     /locus_tag="ACHJZ2_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO4567399.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            51764..53131
                     /gene="degQ"
                     /locus_tag="ACHJZ2_001248"
     CDS             51764..53131
                     /gene="degQ"
                     /locus_tag="ACHJZ2_001248"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO4567400.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            53221..54288
                     /gene="degS"
                     /locus_tag="ACHJZ2_001249"
     CDS             53221..54288
                     /gene="degS"
                     /locus_tag="ACHJZ2_001249"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO4567401.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(54352..55290)
                     /gene="mdh"
                     /locus_tag="ACHJZ2_001250"
     CDS             complement(54352..55290)
                     /gene="mdh"
                     /locus_tag="ACHJZ2_001250"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4567402.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            55725..56195
                     /gene="argR"
                     /locus_tag="ACHJZ2_001251"
     CDS             55725..56195
                     /gene="argR"
                     /locus_tag="ACHJZ2_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO4567403.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        13859 a        15132 c        14013 g        13311 t
ORIGIN      
        1 ttagtttttt ctttttaaat gatgcggcca ttgcgttctc agtaattaaa taacattatc
       61 aatgcgtctc taaacattac gcagaacgta gccatcagta gttatactga caggagcagg
      121 atggtttatc gagaagtaaa catatccgta ttcgtaccgg aactggagtt gacacaataa
      181 agtgccaatt atgtcagcag tagggaaaag tttataacga aggcgtaccg gcaccatcgt
      241 ttcaaggtac cagctacgag taaagcaact ggacgagata cagatacctg acaaccattc
      301 ctcagaccag gaccaaaacg aaaaaagacg cttttcagcg tctcttttct ggaatttggt
      361 accgaggacg ggacttgaac ccgtaagccc tattgggcac taccacctca aggtagcgtg
      421 tctaccaatt ccaccacctc ggcacggaaa ctacttttta gttcgggata tcgctggtcg
      481 gcttagccgg agcagctggc tgagtttgtt cggttttcgc cggcgcactc aggttttccc
      541 attcgctacc tttattggtc ttgttgctat tgatgttacc cagcaccaga ctgatgatga
      601 agaataacgt tgccagcaac gccgtcatgc gggtcatgaa gttaccagaa ccacttgaac
      661 caaacagcgt agcggaagcg cctgctccga aggaggctcc catatcagcg cctttacctt
      721 gctgtagcat gatcagacca acaaggccaa ttgccacaat aaggaaaact accaaaagag
      781 cttcgtacat aatcaacctg ttccttgcgg agttgccgcg taccaatgct tcaaccaata
      841 aagcgggagt ttataatctt tcccactgaa gcgggtgtga atactaacca aaacgaatga
      901 ccttcgcaag ggcaatttta tcgcattgta tcaactgcgg aaaaaaacag caaaaccgat
      961 gtgttcgccg agaaaaaagg cgacaatcgc cgccttttta gccagttatc taacgcttta
     1021 aacggctttt actgcatcgg cgatgcggtg tgcaaattca gtcacctgcg cttcgtcttc
     1081 gccttccacc atcacgcgaa ttaacggttc ggtgccggat ttacgcaaca gcacacgtcc
     1141 acggctaccc agcgccgttt caacttctgc ggtcaccgct ttgactgatt catgctccag
     1201 cggatcgccg ctacctgcgg tgtaacgcac gtttaccagg atttgcggga acattttcat
     1261 gccgctgcaa aggtcgtgca ggctcatatg gttacgcgcc atcgccgcca gcacctgcaa
     1321 accagcaacg atgccgtcac cggtagtggt tttatccagc aggatcacat gaccggaatt
     1381 ctctgcaccg atacgccagc ctttctcctg cattttttcc agtacgtagc ggtcacccac
     1441 tttcgcgcgc gcaaatggaa ttcctaactg tttcagcgcc agttcaagcc ccatgttgct
     1501 catcaatgta cccacagcgc caccacgcag ctggccctga cgaagacctt cacgcgcgat
     1561 gatatacatg atctgatcgc catcgacttt attgccttca tggtcaacca taatcacgcg
     1621 atcgccgtcg ccgtcgaagg caataccgag atccgctttt tcagccagca cacgagcctg
     1681 gagcgcgcga acgtcggtag cccccacttc ggcattgatg tttacaccgt ttggctcaca
     1741 accgatagcg ataacattcg ctcccagttc gcgcagcacg ttcggcgcga tgtgataagt
     1801 cgcaccgttt gcgcaatcca ccacaatctt cagttcactg aggctaagtt cgttcgggaa
     1861 cgtggctttg caaaactcga tatagcgacc cgcagcatca acgatacggc tggctttacc
     1921 cagttctgcc gaatcaacac agctgatctc tttttccatt tccgcttcga tggcttcttc
     1981 aactgcatcc ggcagcttag taccgtcaat agaaaagaat ttaatgccat tatcgtagaa
     2041 cgggttatgc gatgcagata tcacaattcc ggcctctgcg cggaaggtac gcgtcagata
     2101 agccacggcc ggtgttggca tcgggccagt gaagagtgcg gaaaggcccg cagccgccag
     2161 ccccgcttcc agtgctgact ccagcatata gccagaaata cgcgtgtctt taccaataat
     2221 aatcttacgg gagccgtggc gcgccagcac tttacccgcg gcccaaccca gcttaagcac
     2281 aaaatcaggt gtgatcggcg catcccctac acgaccacga atcccatcgg taccgaaata
     2341 tttacgatta ctcatagcgt ttgttttcct ttgcagacag agtagcttcc accactcgca
     2401 tcgcttctac ggtttctttg acgtcatgaa cacgaataat gtgcgcgcct tgcattgcgg
     2461 caatgaccgc acaggccaga ctaccgctca ggcgctcgga cggccccacg ttcagcagct
     2521 gcccaatcat cgattttcgt gacataccca ccaacagcgg caggttgaaa tggtgaaatt
     2581 cagccaggcg cgccagtaat gaatagttat gggagagatt tttaccgaaa ccgaatccgg
     2641 ggtcgagcaa caatttctct tttgcgatac ccgcctgctc gcaacgtgct atttgctcaa
     2701 taaagtagcg attcacttct gcaaagacat cgtcatactt cggggcttcc tgcatggttt
     2761 ttggatttcc ctgcatatgc atcagacaaa ccggtaaacc ggtttctgca gccgcctcca
     2821 gagcgccagg ttcggaaagg gagcggatat cgttaataat gtgagcgcca actttcgctg
     2881 actcacggat gacttctggt ttggatgtat cgaccgagat ccagacttcg aagcgttgag
     2941 caattgcctc aaccacagga ataacacgtt gcaactcttc ttcaacgcta acttccgccg
     3001 cccctggccg cgtggactcg ccaccaacgt caatgatcgt cgcgccagcg ttgatcatca
     3061 gattcgcatg tttcaccgca tctatcagcg agttatgcgt gccaccatcc gaaaaggaat
     3121 caggcgtgac gttgaggatc cccattacgt gaggatggct aaggtccagt gaagtaccct
     3181 gggcaaagag tttcatgatg ttatccctgg tatgaattga taagaaaaaa ccccggagca
     3241 cgccccgggg ttttcggtac aaatacagtc atctgatgcg ggaacttact tgtcgcctaa
     3301 ctgctctgac atggtgttac ccgggttcgg cgtacgcggt tcatcaaccg gacgaggagc
     3361 ctttggacta ccattgtcgc cagcattgtt agaagcgcct ggttcttccc agcccgctgg
     3421 cggacgtaca tcgcgacgtg ccatcaggtc atcaatctgc ggtgcgtcga tagtctcata
     3481 tttcatgaga gcatctttca tcgcatgcag aatatccata ttgtcggtca gaagctgacg
     3541 cgcacgatta tagttacgct caatcagtgc tttcacttcc tggtcgatga tacgtgcagt
     3601 ttcatcggac atatgtttcg ctttcgctac gctacggccg aggaacactt caccttcttc
     3661 ttccgcgtac agcagtggac ccaatttctc agagaagccc cactgagtca ccatgttacg
     3721 tgccaggttg gtcgcaactt taatatcgtt ggacgcaccg gtagaaacat gttccggccc
     3781 gtagatgatc tcttctgcca gacgaccacc gtacagcgta gaaatctggc tttccagttt
     3841 ctgacggctg gcgctgattg cgtcgccctc aggcaagaag aaagtcacac ccagcgcacg
     3901 accgcgtggg ataatcgtca ctttgtgcac cggatcgtgc tccggcacca ggcgaccgat
     3961 aatcgcatga cccgcttcgt ggtaagccgt cgattctttc tgcgcttccg tcatcaccat
     4021 ggagcgacgt tccgcaccca tcatgatttt gtctttcgct ttctcgaact caaccatcga
     4081 cacaacgcgt ttgttgccac gagcagcgaa cagtgccgct tcgttcacca ggttcgccag
     4141 gtcagcaccg gagaaaccag gagtaccacg ggcaatgatt gccgcgtcga tatcgggtgc
     4201 caatggtacg cgacgcatgt gaaccttcag gatctgctca cgaccgcgaa catctggcaa
     4261 gccaaccaca acctgacggt cgaaacggcc aggacgcagc agcgctgggt cgagaacgtc
     4321 cggacggtta gtcgcggcga taacgatgat accttcgtta ccttcgaagc catccatctc
     4381 aaccagcatc tggttcagag tctgttcacg ttcatcgtga ccaccgccca gacctgcgcc
     4441 acgctggcgg cctacggcgt cgatttcatc gataaagatg atgcacggtg ccgctttctt
     4501 cgcctgttcg aacatgtcac gaacacggga tgcacccaca ccgacgaaca tttctacgaa
     4561 gtcagaaccg gagatagtaa agaacggaac tttcgcttcg cctgcaatcg ctttcgccag
     4621 cagcgtttta ccggtacccg gaggaccgac catcaggacg cctttcggga tcttaccgcc
     4681 gagtttctgg aagcggctcg gctcgcgcag atactcaacc agttcagcaa cttcttcttt
     4741 tgcttcgtcg cagcccgcaa cgtcagcaaa ggtcgttttg atctgatctt ccgtcagcat
     4801 gcgcgcttta ctcttaccaa acgacatggc acctttgcca ccgccgccct gcatttgacg
     4861 catgaagaag atccagacac caatcagcaa cagcatcggg aaccaggaga tgaagataga
     4921 agccagcagg ctgggttctt caggcggttc accgacaacc ttgacgttct tggtcaacag
     4981 gttatccagt aatttcggat cctgaaccgg aatgtaagtg gtataacggt tactatcttt
     5041 cttggtaacg ttgatttcac gtccgttgat acgcgcttca cgaacctggt cgttattgac
     5101 ctcttgtagg aaggtagagt aatccacctt acggccatta gactcgctgg gcccaaagct
     5161 ctggaatact gacatcagca caacggcaat gaccagccag agtattaggt ttttcgccat
     5221 gtcactcaag ggattaacct cttattacaa ctgtgttaaa aacagcgtca ggatactcta
     5281 tatccagcat ctttcaaact ttcgtctgaa atctcccggt tagggtttac gcccggtcgc
     5341 tacaatatac acttcccgcg aacgtgcacg agaagagtcc ggcttacgaa ctttgacctt
     5401 cgtaaacagg gagcgaattt cccttagata ctcatcgaaa ccttcgccct ggaacacctt
     5461 cactacaaaa ctgccacctg gcgctaatac atcacgacac atttctagcg ccagttccac
     5521 cagatacatg gcacggggga tatccaccgc cggtgttccg ctcatgtttg gtgccatatc
     5581 ggacatgaca acctggactt tgctgtcgcc aacgcgctcc agcagtgctt tcatcaccag
     5641 ttcatcacga aaatcgccct gaagaaagtc cacaccaacg ataggatcca taggtaaaag
     5701 atcgcaagcg atgatgcggc ctttgccgcc aatttgggtg accacatatt gtgaccaacc
     5761 acccggagca gcaccaaggt cgacaaccgt cattcccggt ttaaagagtt tgtcactttg
     5821 ctgtatttca tcaagtttaa accaggcacg ggaacgtaac ccctttttct gtgcctgttg
     5881 aacatattta tcgctaaagt gttcctgaag ccagcggctg gagctggcag aacgcttctt
     5941 acctgtcatt taactttccc atggggataa ctcatcgtaa ccaattgcgt aaatttttac
     6001 gcgcctattt ggtgatatat gggagatggc ggtagaatga cccgttttca atcccaacgt
     6061 aagcaaaaaa tacgatgaat ctgagtacta aacaaaaaca gcacctgaaa ggtctggcac
     6121 atccgctcaa gccagttgtt ctgcttggca gtaatggttt gaccgaaggg gtgctggccg
     6181 agattgaaca agcgttagag caccatgaac tcatcaaggt gaaaatcgcc accgaagatc
     6241 gcgaaactaa aaccttgatc gtggaagcta tcgtgcgcga aaccggcgcc tgtaatgtac
     6301 aggtcatcgg taaaacgctg gtgctttatc gcccaactaa agaacgtaaa atctcgctgc
     6361 cacgctaaga ttatcctaaa gttacacaca tttgctgtgt aaaacgaggg gttttccgca
     6421 ggcaggagag caaaatgcca cgctctgttc gttgataaaa ggccgctatg cggccttttt
     6481 cctttcttta caatacatca acatcttgag tattgggtaa ttcttacagg tattccacct
     6541 taattacttc aaattctact tcgccgcccg gcgttttgat gaccacaaca tcatcttctt
     6601 ctttgccgat caggccacga gcaataggtg agtttacaga aatcaggttt tgtttaaagt
     6661 cagcttcgtc atcgccaacg atgcgataag tctgttcttc gtcagaatcg agattcagca
     6721 ccgttacggt agcaccaaaa ataacgcgcc cattgttggg cattttggtg acatcaatca
     6781 cctgcgcgtt cgacagcttg gcttcgatgt ctttgatacg gccttcgcag aagccctgct
     6841 gttcacgagc tgcgtggtat tcggcgtttt ctttcaggtc gccatgctca cgcgcttccg
     6901 cgatagcagc aatgatttca ggacggcgca cagatttcag aaaatccagc tcttcgcgta
     6961 atttttcagc gccgcgtaag gtcatcggaa tagcttgcat ttgttatacc tcttgaatat
     7021 tcctgatggg gcaagtgttc acccaccccg gctgttcggc ctgcccggca taactcctca
     7081 ccgggaccag aagcaaaaaa ataccgaccc gggtacaagt cccaggtcag ctacaattca
     7141 cattttgata gtcattttac cctgaagttc ccgaagggtc atcgtttact ttatagggcg
     7201 ttgcgccgta gtatgacggc tcgattccag gttgttagcg cgagattatg cgattttcca
     7261 gatttatcat cggattgacc agctgtatag cgttcagtgt tcaggccgca aatgttgatg
     7321 agtacattac tcaactcccc gctggtgcca accttgccct gatggtgcaa aaagtcggcg
     7381 cgtcggcccc cgctattgat taccacagtc agcagatggc gctgcccgcc agtacccaga
     7441 aagtgattac tgcgctggcg gcgttgattc aactcggccc cgattttcgt ttcaccacga
     7501 cgcttgaaac caaaggcaat gtggaaaacg gcgtacttaa gggtgactta gtggcgcgat
     7561 ttggtgccga tccgacgtta aaacgtcaag atattcgcaa tatggtcgcg actttgaaaa
     7621 aatctggcgt caaccaaatc gatggcaatg tgttgataga tacctccatt ttcgccagcc
     7681 acgataaagc ccccggctgg ccatggaatg acatgacaca atgctttagc gctccgcctg
     7741 ccgtcgccat agttgaccgc aactgtttct ccgtctcgct ctacagtgcc ccaaaacctg
     7801 gtgatatggc ttttatacgc gtggcatctt attaccccgt tacgatgttc agccaggtac
     7861 gcaccctccc ccgtggttct gccgaagcgc aatactgcga actggatgtg gtgccaggcg
     7921 acctgaaccg ctttacgctg acgggatgcc tgccacaacg ttctgagccg ctcccgttgg
     7981 cttttgccgt gcaggatgga gccagctatg ccggtgcaat tctgaaagat gagttaaaac
     8041 aggcgggtat cacctggagc ggaacactgc tgcgccagac tcaggttaac gaacctggaa
     8101 cggtagttgc cagtaaacag tcggccccgc tgcacgatct gcttaagatt atgctgaaaa
     8161 agtcggacaa catgatcgcc gatacggttt tccgcatgat aggccatgcg cgcttcaatg
     8221 tgcctggaac atggcgggcc gggtcggacg ctgtgcgtca gatcctgcgc cagcaagccg
     8281 gtgtcgatat tggaaacacc attattgccg atggttcagg gctttcgcgg cataacctga
     8341 ttgcccccgc caccatgatg caggtgctgc aatacattgc ccaacacgac aatgaactta
     8401 actttatctc catgctgccg ctagcgggct atgacggctc tttgcagtac cgtgcaggtc
     8461 tgcatcaggc gggcgtggat ggaaaagtct cagcgaaaac cggttcgttg cagggggtat
     8521 ataacctggc gggattcatt accacagcga gcgggcaacg aatggcgttt gtacaatatc
     8581 tttctggcta tgcagtagaa ccggcggatc agcgtaatcg ccgtattccg ttagtgcgtt
     8641 ttgaaagccg tttgtataaa gatatttatc agaacaatta gtcaaaaaga aaccccggca
     8701 catggccggg gttttgctga ttaacgcttg taaatgaact caacgccttc ttcgtcgtct
     8761 tcgtcccagt cgtcatccca gtcctcatca tcctcttcag caatctcttc aagctgctgg
     8821 cgatgataat catcccacat gaattcgact ttctctggct gtttcgcttc ttcagcctgc
     8881 acgaccgggt tttcaatgat aaaggtcatc acatcccagc agagatcttt cacgcccagt
     8941 ccactcgccg cagagatcag ataatattta tcttcccagc ccagcgcctc tgcgatcgct
     9001 ttcgctttct cttcggcttc taccttatcc agcagatcga tcttgttgaa gactaaccaa
     9061 cgcggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat aatacgcgcg
     9121 ttttcaaccg gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa caggacgcgg
     9181 caacgttcca ggtgcttcag gaagcgaatg cccagacctg cgccttccgc agcgccttca
     9241 atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg taccacaccc
     9301 agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc cgccgatacc
     9361 gcacgaataa aggtcgattt acccgcgttt ggcatcccca acataccgac gtcagccagc
     9421 agcatcagct ccagcagcag ctcacgctta tcgcccggcg taccgttagt tttctgccgt
     9481 ggtgtacggt taactgacga tttgaaacgg gtattgccca gaccgtgcca gccgccctta
     9541 gcaaccagca gacgctgacc gtgtttggtc atatcgccca tggtttcacc agtaccctgg
     9601 tcgattacac gcgtacctac cggcacttta attgtgacgt ctttaccgcg cttaccggta
     9661 cagtcgcggc ttgcgccatt ctgaccgcgc tctgcacgga aagatttttc aaaacgataa
     9721 tcgataagcg tgttcaggtt ctcgtcggct tccatccata cgtcaccacc atcaccgccg
     9781 tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct cacgcaacca
     9841 ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt cattttattc
     9901 tccgtaaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt acgtaatccg
     9961 ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac aaacgcaccg
    10021 agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc cagtgataaa
    10081 agatctgaaa aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct cacctgcgct
    10141 gcctgccagc gagccatcgc ttccgccagg gcgccatatc ctaccaaggt attcagtccg
    10201 caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc aggctttgcc
    10261 agagggaaga gcgcaattgt acataaagtg tacagtaaaa acaggatctg cggtgaggcc
    10321 agccgacgca ataaaacctt ttgcgccacg ccataactca cccaaaccgt cgcagcaccg
    10381 accccaaaga taactcccca ggtgtaatcg gttagctttg taaatatctc gaccagactg
    10441 gtgttaaaaa acatcaccag gccgctcagg agcatcaatg cccctacaac ctgagtgctg
    10501 cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg cgagagttgc
    10561 ccaatcacct gcgaagcggt cggactcagg tattgcaagg atgagctgaa caggatgaag
    10621 ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg cttacgaaac
    10681 acgcgtaatg gcggcaacct cttcttcacc gcaagaatgg cccccaggcc aatactcgcc
    10741 atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac ctgcttcatt
    10801 gcaattggca acgcccccca gcaaattgct gtggtgagcg ccaaaagaat gccaatgcct
    10861 gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg tcgcaccatg
    10921 cgcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg gctttcatcc
    10981 gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg gtttttcggg
    11041 cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg gtcacgaccg
    11101 caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat gatgctaccc
    11161 gccagaacgg attcgccacc gaaacgctta acgcccaggc gtttagcttc tgaatcgcga
    11221 ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc ctcaggtctt
    11281 aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatggccc tgctgcttac
    11341 gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga ccgtgagcaa
    11401 caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg acttcttcac
    11461 cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg atgtccagct
    11521 tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca ctttggaaaa
    11581 ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag agtgcgctat
    11641 aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca agtgctacag
    11701 tcaatacacg aagaaaaaaa acacaacttg tacggtaacg tttatctgtg ccattttttc
    11761 agtacaatca ccctatattc ctaaccataa accctaagtt gcctttgttc acagtaaggt
    11821 aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg agttaaccgc
    11881 gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg acgtccaact
    11941 gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc gtccgatgat
    12001 tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca ccattgctgc
    12061 cctgatcgag tttatccaca cggcgactct gctgcacgac gacgttgtgg atgaatcaga
    12121 tatgcgcagg ggtaaagcta ccgccaacgc cgcatttggc aatgccgcca gcgtgctggt
    12181 aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggtt cactcaaagt
    12241 gctggaagtc atgtcagaag ccgtaaacgt catcgcagaa ggtgaagttc tgcaactgat
    12301 gaatgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct atagcaaaac
    12361 cgcgcgtctg tttgaggctg ccgcgcagtg ttccggaatt ctggcgggct gtacgccgga
    12421 ggaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc agttgatcga
    12481 cgatttactc gattacaatg ccgatggcga acagttaggt aaaaacgtcg gcgacgatct
    12541 gaacgaaggt aaaccgacgc tgccgctgct gcatgcgatg catcatggca caccagaaca
    12601 ggcacagatg atccgtaccg ccatcgaaca gggtaacggt cgccatcttc tgaaaccggt
    12661 tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgcg ccgaggaaga
    12721 agcagacaaa gccatcgcag cgttacaggt gctcccggac accccttggc gagaagcact
    12781 catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctcccc tcatcccgcg
    12841 cagtgcgcgg gatgattcca gtaaactcca taagaatacc tattcagcgt aataatgcac
    12901 tgaaaaacca gtgcatacat ctttatatat tctgaatatt cacactcttt ataggaactt
    12961 tttagagcaa tcggccatca ggagtatagt gatgctcgac agaagaagtg ttctgaatga
    13021 aagcgactaa cttaaggagt gaggaaagat ggaaagtaat ttcattgact ggcatcccgc
    13081 tgacatcatt gcgggtttgc gcaagaaggg aacatcaatg gcggcggagt ctcgcagaaa
    13141 tggtttgagt tcctcaacgc tggcgaatgc attatcgcgc ccatggccga aaggagagat
    13201 gattattgcg aaagccctgg gaactgaccc ctgggttatc tggccatcac gctaccatga
    13261 tccgcagacc catgagttta tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa
    13321 aaaatgagtg gtctggcggt agccccgcga acggggctgc cagctctcag acgattattc
    13381 gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta
    13441 tccacgatcg atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa tacagccagc
    13501 cagcaccagg cttgctgatg cacgcagatc ggttgccata acctgtgcgc cagaaagttt
    13561 ttcaacaccg tgacaaataa cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct
    13621 cagctctggc acatgcataa agcggttttc aaagaccgtt tcggtgatga atccagtccc
    13681 ttctgccacc aggttcaaca gcgtgaactg ggcctgcata tcggtcggga atgccggatg
    13741 cggcgcggta cgtacgttaa cagccttcgg acgtttgcca tgcatatcca ggctaatcca
    13801 gtcttcgccg acctcgatgt ccgctccagc gtcacgcagt ttcgccagca cggcgtcgag
    13861 agtatctggc tgcgtgttac ggcagataat tttgccgcga gaaatcgccg ccgccaccag
    13921 gaaagtaccg gtttcgatac gatccggtag cacgcgatag acaccgccgc ctaaacgttc
    13981 cacaccttcg atgacgatac gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat
    14041 caggaagttc gcggtatcga cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt
    14101 gccttccgcc agggttgcag cacacatgat ggtcaccgtt gcgccaacgc tgactttatc
    14161 catcacgata tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt aaccttcttc
    14221 cagtttgatg gtcgcgccta attgttcgag gccagaaatg tgtagatcaa ccggacgcgc
    14281 accgatcgta caaccgccag gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg
    14341 ccccagtgcc cagatagaag cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac
    14401 attaacgtcg cgggcatcaa tatgcacaga accattacgt tctactttcg cacccagctg
    14461 gcttagcagc ttcattgatg tatcgacgtc tttcagtttc gggacgttct ggatctctac
    14521 cggttcttcc gccagtagag cggcaaaaag gataggcaga gcagcatttt tagcgccgga
    14581 aattgtgact tcgccctgga gcttcgttgg cccctgaaca cgaaatttat ccatttagtt
    14641 tgttctcagt taacaattca tatccgctac cggcgaatcg cccatagctc aaaagccgtt
    14701 cagtttgcga tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca cagcatgaat
    14761 gcggttatcc gcaatatatt ccatcagcgg accatagacc gtctgctgtt ttttaacccg
    14821 actcatgccg tcaaacaact cacccacggc aataacctga aagtggctgc catcgccgga
    14881 aacgtggact tcctggaggg agagagcgtt catcaacacg ctctgaattt cattattttc
    14941 catgggatct tcaatcatca gttaataaac cagcgaaaca tcttagagca aagttgcgct
    15001 ggcataaata agcaaaaagc ctcgctgata aatcagacaa ggctcgactt gcaggcaggt
    15061 ttgccggaca ggcggttaac gccatatccg gcctgaaaaa atttaacgag gcagaacatc
    15121 agcaggcaaa ttatacaatt tcgccagggt atacactttg tcgtttaccc cctgaagcgt
    15181 cacgttgttg ccctgctttt tcgccagatc gataagatgg agcagcagtg ccagtccacc
    15241 cgtatccacg cgggacacac ggctaagatc gatgcaggta atccccttca ccgcttcctc
    15301 acgcatttcc caaagcggta gcagaacgtc ctgatccagc tctccggata acgccagtgt
    15361 gtcacccgtc tgcatccagc tcagtgactc gctcattatt ttttctcttc cagagtgatt
    15421 ttctgttgag aaatcgattt cagttgcgca gtcaggccgt cgataccttt ggtacgtagc
    15481 agcgttcccc actcgttttg tttggtggtg atcatactga cgccttcagc aatcatgtcg
    15541 taagcctgcc agttgcccgt ctgggagttt ttacgccact ggaagtccag acgcaccggc
    15601 gggcggccat tcgggtcaat aatggtaacg cgaataggca caatggtttt atcacccagc
    15661 ggctgttctg gcgcaatctg ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa
    15721 gcctgcttca ggtactcacg gaaagcggca aagtaagcat cacgttgagc tggggtcgca
    15781 ctcttgtaat actggcccag caccagcgca ccggcgtatt tcacctgtac gtatggcagc
    15841 agttcctgat caacaatggt acgcagataa tccgggttgg cccgaatttg cggttgctca
    15901 ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata cggattggtc
    15961 tggtctgccg cggttgccgc actcagcggt gcaatcacca gcaaagcgac catcattaaa
    16021 cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg ttcagtggtt
    16081 tcattattac ctggcgcagc agctggcgca tcgccactat tcttattgtc atcgccttta
    16141 ctaccgtaaa ggaactgacc aatgagatct tccagcacca tcgcagactt ggtgtcctga
    16201 attgtatcgc catccttcag gatagcagtc cccagttccg ggtcttcaaa accgacgttt
    16261 aatgccagat attgttcccc cagcaggccg gaagtacgaa tgctcagcga actggtatca
    16321 ggaatatggt tataacgttg ttcaatttcc agcgttacgc gcggcagata ggttttcggg
    16381 tccagcgtaa tatccgccac ccggcccaca acaacgccac caatactgac cggagagcgc
    16441 gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg ttcagtacgt
    16501 attgacgtca cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag tgctgctaat
    16561 aaaaagatac ccacccaaat ttcatttttt ttcgtttgca tgaactcaat tcccaaacat
    16621 caatgcggtc agcacaaaat ccagccccag aacagccaga gacgagtgga caacggtgcg
    16681 agtggttgcc cggctaatcc cggcagacgt cgggatggcg tcgtagccgt taaacaacga
    16741 aatccacgtc accgtgatgg cgaacaccac gctcttaatc agacagttga ccagatccat
    16801 acgccagtcg acggcatttt gcattgccga ccagaagaac ccgctatcaa tgcctttcca
    16861 actgacgccg accagcgatc cgccccagat ccccacggcg acgaagataa ccgtcaacag
    16921 tggtaatgaa ataaccccag cccagaaacg gggagaaata acccgacgca gcggatccac
    16981 cgccatcatc tccatactgg agagttgctc tgtagcgcgc atcaggccga tttctgcggt
    17041 tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca accaccggcc ccagttcacg
    17101 cagtagcgat aacgccacca gcatacccag actggtttcc gcactataag tggtcagaac
    17161 cagataacct tgcagcccca acaccattcc gatgaacacg ccagaaacta caataatcag
    17221 catcgacagg acgccgacat tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa
    17281 ttccggtttg ccgaccagcg cattgaataa cattaacccg gcccgcccaa acgttctcag
    17341 ggttttaatc cctttatgtc cgagcgacgc cagcgcattt aacagcatga gtggcttaac
    17401 tccctggtaa aagatcagcg tgataatcgc cggcaggata gcggaacgga accggcccgt
    17461 cagctatccc gtccagaaac tgacgtacgc gcggatcagg attcgcctgc aacgcctggg
    17521 cactgccatg agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc gcaatactta
    17581 acacttccgg cacatcgtga gaaaccacca cacaagtcac gcccagcgcg ctgttcagct
    17641 cagaaatcag cttcaccagt acgcccatgg tgatgggatc ttgcccaaca aaaggttcat
    17701 caaacatgat gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca
    17761 tcccaccgga aagttcagaa ggcattagtt tagccgctcc acgcagcccc acggcctcca
    17821 gcttcatcat caccgtacta tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg
    17881 gataggcgac gttgtcaaat acgttcatat cagtgaacaa cgccccggac tgaaataaca
    17941 tgctcatccg tttgcgcact gtatacaggc gcgaacgaga catcgccgga atattctcac
    18001 catcgaaaag gatctcacca tgatctggtg cgatttgccc gccaatcaga cggagtagcg
    18061 tcgttttacc gatgcccgat ggccccatga tcgccgtgat cttccctcgc ggcacggtca
    18121 gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa actgacatcg cgcatatcga
    18181 ctaaattcgc cacagactgc tccataattc acccttcgtc ttgcgttgat tttctaagca
    18241 tggcgctcaa tttaaccttg aacccaacat atttacagaa tattacccgc cgtggttagc
    18301 gaaagctggc atttgtttta ctttttagcc gcataaagtc aaaattaaga atccgttacg
    18361 gctttctgaa aatcttcagc ggaccggcga gtatacctga agaaaggacg ttagatgctt
    18421 ttagctacgg cactgttaat tgttggttta cttttggtcg tttacagtgc cgaccgcctg
    18481 gtttttgccg cgtctattct ttgccgaacc tttggcatcc cgccgctgat catcggcatg
    18541 acggtggtca gtattggtac atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg
    18601 cacgaacaac gcgatttagc cgttggtaca gccctcggct caaacattat caatatattg
    18661 ctgatcctcg gtctggctgc gctggttcgt ccttttaccg tccattccga tgttctacgc
    18721 cgtgaattac ccttaatgtt gttggtcagc gtggtggccg gttccgtact ctatgacgga
    18781 caacttagtc gcagcgatgg tatctttctc ctctttctgg ctgtgctatg gctgctgttc
    18841 attgttaaac ttgcacgtca ggctgaacgt caggggactg acagcctgac cagagagcag
    18901 cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct cggcattgcg
    18961 cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt gctggcgaat
    19021 tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg aaccagcctg
    19081 ccggaactgg caaccgcaat agcgggcgtt cgcaaaggtg aaaacgacat tgctgtcgga
    19141 aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata
    19201 acgccaggag agattgatcc actggcgtac agtcgtgact acagcgtgat gttgctggtg
    19261 agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg
    19321 gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt atcgccaata
    19381 ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg gttttgactt
    19441 tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca
    19501 atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt
    19561 tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg caacgtttgc
    19621 cagcaccggt acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg gtgatttagg
    19681 catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat ccagcgaaat
    19741 cactgcctta attccagtgc ttaagcgtct tcacataccg ttaatctgca tcaccggtcg
    19801 cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga
    19861 agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg ttatgggcga
    19921 tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt ttgcgctctc
    19981 acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac
    20041 gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcat tgctggaagt
    20101 tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga ttgaaggcat
    20161 ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc gtcagttaag
    20221 tattgccgat gtgatgacgc cggggggaat acgcgtgcgc cctggcattc tggccgttga
    20281 ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg atggcgacca
    20341 tttactcggt gtgttacata tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa
    20401 ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc
    20461 gccgacgtta tagcaaaagc agagaacatt cgtctgctaa tcctcgacgt tgatggcgta
    20521 ctgtcagatg gcctgattta tatgggcaat aacggcgaag agctgaaagc gttcaatgtt
    20581 cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc tatcattacc
    20641 gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac tcacttgtat
    20701 caggggcagt caaacaaact gatcgccttt agcgatctgc tggaaaaatt ggcgattgcc
    20761 ccggaaaatg tggcttatgt cggcgatgat ctcatcgatt ggccggtaat ggaaaaagtg
    20821 ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc cgattacgtg
    20881 acgcgcattg ctggcggtcg tggcgcagtg cgagaagttt gcgacttatt actcctggcg
    20941 cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga cgttgggtta
    21001 tcattgtgct atcactggcg tttctggtga tgatcggtat taatatggcc gaaaaagacg
    21061 ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag catacggaca
    21121 cgctcgtcta taacccagaa ggggcgctaa gctatcgatt gattgctcaa cacgttgaat
    21181 attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg tttgataagg
    21241 ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat gaccggatgc
    21301 tctatttata tggacacgtt gaagtcaacg cacttgtgcc ggactctcaa cttcgcagaa
    21361 tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct gaagacctcg
    21421 tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc aacttacgca
    21481 gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa
    21541 ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc ggcatttgcc
    21601 gtaaccggag acactgatca gccgatacac attgaatcgg accagcaatc tcttgatatg
    21661 caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac cataaaaatt
    21721 aatgccgaca aagtggtcgt tacccgtccg ggtggcgaac aaggtaaaga agtgattgac
    21781 ggctacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc cgttgaaggt
    21841 cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac gggtaatgct
    21901 tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct ggtgaaagag
    21961 cagaaaatgc aggctttcag cgacaaaggc aagcgtgtaa ccaccgttct ggtgccgtcg
    22021 cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg taattaattc
    22081 gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta
    22141 gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg gccaaacggt
    22201 gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga tgcgggcaac
    22261 atcattattg atgatgacga tatcagtctg ctacctctgc atgcacgcgc gcgccgcggt
    22321 atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta cgataacctg
    22381 atggcggtac tgcaaattcg tgacgacttg tctgctgaac agcgtgaaga ccgcgcgaac
    22441 gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca gtcactctcc
    22501 gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt
    22561 ctgctcgacg aaccgtttgc cggggttgac ccgatctcag ttatcgacat taaacgcatc
    22621 attgagcacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa cgtgcgtgaa
    22681 acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat cgcccacggc
    22741 acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg ggaagacttc
    22801 agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt ctgaacatga
    22861 agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag ctccaacagg
    22921 caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag caggcgctgg
    22981 agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc cgcgaaacgc
    23041 aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg ccggaagagc
    23101 tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc ggcaccagcg
    23161 gtgactacat tgacgacgag ctgccggtct atcagggcga aacgacgcag accttgcagg
    23221 attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc gctattgcta
    23281 cctctatcgt cgatgccgtt gatgacaccg gttatctgac tgtcccgctg gaagatattc
    23341 tcgaaagtat gggcgatgaa gagatcgaca tcgacgaagt tgaagccgtc cttaagcgga
    23401 tccaacggtt tgatccggtc ggtgtggcgg caaaagatct gcgtgactgc ctgctgatcc
    23461 aactctccca attcgataaa accacgccgt ggctggaaga ggccagactg atcattagcg
    23521 atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc acgcgtctga
    23581 aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg cgccccgggc
    23641 aatcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg cgtaagcata
    23701 acggtcactg gacggtagaa ctcaacagtg acagcattcc tcgtctgcaa atcaaccagc
    23761 actacgcctc gatgtgcaat aacgcgcgca atgatggtga cagccagttt atccgcagca
    23821 atctgcaaga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat acgctactgc
    23881 gcgtgagtcg ctgtatcgtt aaacagcagc aagccttctt tgagcaaggc gaagaatata
    23941 tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa tcgacgatat
    24001 ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa ctgaagtatt
    24061 tcttttccag tcacgtcaat accgagggcg gcggcgaagc ttcctccacg gcgattcgtg
    24121 cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc gacagcaagt
    24181 taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt gcgaagtacc
    24241 gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat
    24301 aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac cgaggcactg
    24361 cgcgaatttg ttacagccaa atttgccaaa cttgagcaat attttgaccg aatcaaccag
    24421 gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac actgcatgta
    24481 aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc
    24541 ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa acaacactaa
    24601 ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta
    24661 cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg tgaaattatg
    24721 acaaataatg atacaactct acagcttagc agtgttctta acagggaatg tacgcgaagc
    24781 cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa
    24841 caacttagcc taccgcctca ggtggttttt gaagctatcc tgacgcggga aaaaatgggc
    24901 agtaccggta tcggcaatgg tattgccatt ccgcatggca aactggaaga agatactctg
    24961 cgtgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc catcgacaac
    25021 caaccggtgg acttactttt tgccctgctg gtgccggcag accaaactaa aacgcacctg
    25081 catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc
    25141 gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccaaagg tactccggat
    25201 gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca gtacatggta
    25261 ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa
    25321 gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga tctggctcga
    25381 accctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa tatgccggag
    25441 tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc accgcaacta
    25501 ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac gcgccgactg
    25561 catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga aagcgatctg
    25621 ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat gtccgttcac
    25681 gagctggcag aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga actgacaatg
    25741 gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta cgtctttgac
    25801 gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac aggtcttgat
    25861 aagcctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat ctaccagacg
    25921 cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag ctacttgacg
    25981 gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga gcaactggca
    26041 gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt
    26101 aaaccatgac tgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg catgcccggc
    26161 ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg
    26221 acgaaggcac cgaggctgaa gccaacagcg ttattgcgct gctgatgttg gattctgcca
    26281 aaggacggca gattgaagtt gaagcgaccg gtccacagga agaggaagca ctggccgccg
    26341 ttatcgccct ctttaattct ggttttgatg aagattaatc ttcatcactt tgacatacaa
    26401 ctcccttcaa actccccccg acaataagaa aatcacgtac tgaaatcgtt ctcaatcaac
    26461 gtcaattgta catttcgtgc gctttttact ttcagaagaa ccttaagaaa accttaagaa
    26521 gcattgttta ggtttcgttt aagttaatcg accatactgg agatcgtcag aaaatatttc
    26581 caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga agacatcgga
    26641 aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac catcgtggcg
    26701 atggtggcga taaagagatc cgtcgtgagt taaagtacta cgcgcatctt ggtcgccggt
    26761 taaaagactg gagtggaata ccgcgctatc acggtaccgt cgagacggat tgcggaaccg
    26821 gttatgttta tgatgtgatt gctgattttg acggcaagcc ctccattacg ctgaccgaat
    26881 ttgctgaaca atgccgttac gaagaagacg ttgcacaact gcgccaatta ctgaaacagc
    26941 tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg cagaatatcc
    27001 tctgtcaccg cattagtgag tcagaggtta tccccgtcgt ctgcgacaac attggtgaaa
    27061 gcacattgat tccactggcg acctggtcaa agtggtgttg tctgcgtaag caagagcggt
    27121 tgtggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa gatctgcaac
    27181 ctcgtgagag caaaacgctg gccctcgctt cgcgcgaagc ttaatccagc tggtgttgtt
    27241 gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc tgacggctgc
    27301 gcacgtagcc cgatggcgcg gagactttga aacgaagtgg attaggtaat acagctgcca
    27361 gtaaggcagc ttccgatcgg gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg
    27421 cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa accgtcagga
    27481 tacgcttttt gctccagaca gtttctatcc ccagcgttaa tccggcttcc agcccttttc
    27541 gaacccagct acgcccatcc cataaaaaga gatttttggc tgtctgttga gaaatcgttg
    27601 aagcaccgcg aatacggttt tcattgcgct cattgtgcgc cagggctttc tcaatggaag
    27661 cgacatcaaa gccccagtgc tcaggaaatg tctgatcttc tgcggcaatc accgccagtc
    27721 ccatccacgg cgagatttga tccatactga ccctgtcaga atgtgccacg taacgaaaat
    27781 taccatgcag ccaggcgctg acctgtcgct cgaccattac cgcagagaag ggaacaggtg
    27841 caacgctaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc attaaccgca
    27901 gaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt cagccagaac
    27961 cagcacgcga gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag
    28021 catatatgcc ggggtggtga caatcttatt gtcttcatca accacgatat catcgacagg
    28081 acacggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga tatcagtacc
    28141 gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg caatacacat
    28201 aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta attcacggtc
    28261 aacggtgcat tcgcgaccaa gactggcaaa attgcttaaa ttctttgccg cgccaaaccc
    28321 ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag gacggatttc
    28381 accgcgcgtt attcgtgccg cttcaatcag cacattgcgc gtttccgtca tcgcttcgcc
    28441 agttaaatga ttgataacat caacctgctg cttatccggt gcaaagcaga ccgcctgcgc
    28501 accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag aaccgtcata
    28561 gacgccgcat ccgctcagaa ttacgccaat tttcttcatt atgatcatcc tttcgcaatt
    28621 gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt aactgattca
    28681 tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa acagtgcagt
    28741 gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg tgttggcgca
    28801 gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc acccacgctt
    28861 tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat tcaccgacgt
    28921 tatcttccca ggcgggaagg tcaggagact gaatttgctg acccagctgt tgcagatggc
    28981 gcaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca atgccttttt
    29041 tatcctgcgc cgtcagattc gactccagca cgttgacata gcctggcatc atcttctcaa
    29101 acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac tgttccagca
    29161 tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc gtactctcct
    29221 catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga acagaaagcg
    29281 gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct ttcagcacgt
    29341 tagcggttaa ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc gtcagttcac
    29401 gagagatatc cagcccggtc atatctggca actgaatatc cagcaacacc aggtcgtatt
    29461 cgcccggttt aaacatctcc agcgccgcct tgccggtcat ggcgacatca acgctgttac
    29521 ctaatttttc cagcacagaa cgcgcgacaa tcacgttcag ttcaatgtct tccaccagca
    29581 gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg
    29641 ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttaccctgt tcgctggtaa
    29701 ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga ccaataccgg
    29761 tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg gcgaagattt
    29821 tatccagctc atcctgcgga atgccgatac cagaatcttc cacttcaaaa tgcagcatat
    29881 cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt
    29941 tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg acctgatgcg
    30001 gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg
    30061 agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta tcaagctgga
    30121 ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc cccagcgtga
    30181 cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt tcggtatcca
    30241 gcagaatgcg gctcaggccg acgataccgt tcagcggtgt acgcaattcg tggctgatgg
    30301 tggaaataaa cgtcgttttg tcgcggctgg cgcgttcaag cgcatcctga taccgcttac
    30361 gctcggtaat gtcgcgacca aagcccatca aaccgtgacg tttacccacg cggtcgtagt
    30421 acggcacttt acgaatttca aagcaggctt tgcgtccgtc gggataatcc agccactgct
    30481 cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg actttcgcag
    30541 cggcttccgg tgagtaaaca tcagcaggtt tcaggtgaac cagttgtttt tcgctttttc
    30601 cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg ttacgataaa
    30661 aaaccaggtc gggtgaagca tcaaggaagg aacgtaaaaa tgaggattgc tgctcgagct
    30721 gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag gtttcctgca
    30781 gttctgcttc cgctttttca cgaacggcaa tttcctgatt tagctgggca atattatctt
    30841 ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc
    30901 gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga aagtagaccg
    30961 cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat aacatcaatg ctttcgacct
    31021 gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg acgagggcca
    31081 gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca acataatact
    31141 gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg ccccaataat
    31201 acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc cctcaacgac
    31261 ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc catgttcatt
    31321 cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca gaggagcaag
    31381 cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg gtggcgtatg
    31441 gcgaatcatt tctccggcag tgagcgtgta atcctccagt tcaataccgt tcaaacgccc
    31501 cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcagcggat gcagctttat
    31561 gccgtccacg ccggtttcaa ccactcgctc cagcgtttgc aaacattcag cctgcccttc
    31621 gccaggcagg ccgacaatta agtgagaaca caccttcagc ccgcgctgac gtgccagctg
    31681 ggttgtacgc tgataacagg caaaatcatg gccgcgatta atgcgatgca gggttttgtc
    31741 gtgggcggtt tgtagcccca gctccagcca cacttcgtag ccctggtcct tatattcgca
    31801 aagcagatcc agcaccgcat ccggcacgca gtccgggcga gtaccaacac acaaaccgac
    31861 aatattggcc tggctcaccg cctgctgata catagaacgc agcacctgaa cttccgcaaa
    31921 ggtgctggta tacgcctgaa agtaggccag atagcgttta gcgcggttaa ctaaattcgc
    31981 ctggtgcgcc agttgctcgg caatggaacg atgttgctgc gcttcatcgg caaatgaggc
    32041 aacattacag aatgtgcagc cgccacgccc gatggtaccg tcacggttag ggcagctaaa
    32101 accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga gatcaccacc
    32161 aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt gaaaagaggc
    32221 caaagcctgc catttttagc tcaattcggc gatgacttgg atcaatcgtc ccggcctgct
    32281 tttatcaact gcataaccaa tcaaaattac cgaaatttca tgcataatca cataaatcac
    32341 ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca aaacagtagc
    32401 atgaaacgtc attaccaatt aaggcagtat aaaatgctgg ttttctcgtc agttcaaggc
    32461 aggataaggg ttaacacgcc tttatgacag tcaggaattg gctgtttctc taacgacgcc
    32521 ccttttagcc ttaattagaa aatccatttt aatttcagtc aattaataaa gaattttgcg
    32581 ctaaagcaca tttctgtacc aataagcttg ccatttgacc tgtatcagct ttcccgataa
    32641 gttggaaatc cgctggaagc tttctggatg agcagcctgc tcatcatatt tatgcagtaa
    32701 ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg acaacaaggg ggcgaatgcg
    32761 aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt tctgtgcccg tgcgcagcgg
    32821 ttcggaagtg gggttcccgc agagcctggg ggaggttcac gatatgttgt acgataaatc
    32881 ccttgagagg gataactgtg gtttcggcct gatcgcccac atagaaggcg aacctagcca
    32941 caaggtagtg cgtactgcaa tacacgcact ggcccgcatg cagcaccgtg gcgcgattct
    33001 cgccgatggt aaaaccggtg acggttgcgg cttgctgtta caaaaaccgg atcgcttttt
    33061 tcgcatcgtt gcgcaggagc gcggctggcg tttagcgaaa aactacgccg tcgggatgct
    33121 cttcctgaat aaagatcctg aactcgccgc tgccgcacgc cgcatcgttg aagaagagct
    33181 gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc cccactaacg aaggcgtgct
    33241 gggtgaaatc gccctctcct ctctgccacg cattgagcaa atttttgtga acgccccggc
    33301 aggctggcgt ccgcgcgata tggagcgccg tctgtttatc gcccgccgcc gcattgaaaa
    33361 gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg tcgaatctgg tgaacatcta
    33421 taaaggtctg tgtatgccgg cggatctgcc gcgcttttat ctagatcttg cggacctgcg
    33481 tctggaatcg gccatttgcc tgttccacca gcgcttctcc actaacaccg taccgcgctg
    33541 gccgctggcg caaccgttcc gctacctggc gcataacggt gaaatcaaca ccatcaccgg
    33601 taaccgccaa tgggcgcgtg cgcgtactta taaattccag acaccgctta tccctgacct
    33661 gcacgacgcc gcaccgttcg tcaacgaaat cggctctgac tccagttcga tggataacat
    33721 gctggaactg ctgctggcag gcgggatgga tatcatccgc gccatgcgtc tattagtacc
    33781 acccgcctgg cagaacaacc cggatatgga cccggaactg cgtgccttct ttgactttaa
    33841 ctccatgcat atggagccgt gggatggccc ggcgggcatc gtgatgtctg acggtcgttt
    33901 tgctgcctgt aacctcgacc gtaacggtct gcgtccggcg cgctacgtca tcaccaaaga
    33961 taagctcatc acctgcgcct ctgaagtcgg tatctgggat taccagcctg acgaagtggt
    34021 cgaaaaaggc cgcgtcgggc caggcgaact gatggtaatc gacactcgca gtgggcgtat
    34081 tctgcactcg gcagaaaccg atgacgatct gaaaagccgc cacccttata aagagtggat
    34141 ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg cccgatgaag aagtgggtag
    34201 ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa cagtttaact acagcgcgga
    34261 agagctggac tccgtaattc gcgtactggg cgaaaacggt caggaagcgg tcggttcgat
    34321 gggcgatgat accccattcg ccgtgctctc cagtcagccg cgcattattt acgactactt
    34381 ccgccagcag tttgcccagg tgactaaccc gccaatcgac ccgctgcgtg aagcgcatgt
    34441 tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc ttttgcgaag cagagggcca
    34501 ggcgcaccgt ttaagcttta aatcgccgat tctgctctac tccgatttca aacagctcac
    34561 gacgatgaaa gaggagcact accgcgcaga tacgctggat atcacctttg acgtcaccaa
    34621 aaccacgctc gaagcgacgg tcaaagagct gtgcgacaaa gccgaaaaaa tggtacgtag
    34681 cggcaccgtg ctgctggtgc tctccgaccg gaatatcgct aaagatcgcc tgccggttcc
    34741 ggctccaatg gcggttggcg cgatccagac ccgtctggtc gatcaaagcc tgcgttgcga
    34801 tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg caccacttcg ccgtgttgct
    34861 gggtttcggc gcgacggcta tttatccgta ccttgcctat gaaacgctgg gccgcctggt
    34921 agacacccac gcgattgcca aagattatcg taccgtgatg ctcaactacc gtaacggcat
    34981 caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc tccaccatcg cctcttaccg
    35041 ctgctcgaaa ctgtttgaag cggtcggtct gcacaatgat gtagtgggcc tgtgcttcca
    35101 gggggcggtc agccgcattg ggggggcgag ctttgaagac ttccagcagg atctgctgaa
    35161 tctctcgaaa cgagcctggc tggcgcgcaa gcccatcagc cagggcggcc tgctgaaata
    35221 cgtccacggc ggcgaatacc acgcctacaa cccggacgtg gtgcgcacgc tgcaacaggc
    35281 ggtacagagc ggcgagtata gcgactatca ggaatacgcg aagctggtta atgagcgtcc
    35341 ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt gaaaacgcgg tcaacattgc
    35401 cgatgttgaa ccggcaagcg aactgtttaa acgcttcgat accgcggcga tgtctatcgg
    35461 cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg atgaacagca tcggcggaaa
    35521 ctcgaactcc ggtgaaggtg gtgaagaccc ggcgcgctac ggcaccaaca aagtgtcgcg
    35581 catcaagcag gtggcttccg gtcgctttgg ggttactccg gcgtatctgg tcaatgccga
    35641 cgtcattcag attaaagtcg cccagggcgc gaagccgggc gaaggcggtc agttgccggg
    35701 cgacaaagtc actccttaca ttgccaaact gcgctattcg gtgccgggcg tgacgctgat
    35761 ctccccgccg ccgcaccacg atatctactc tatcgaagac ttagcgcagc tcattttcga
    35821 cctcaagcag gttaacccga aagcgatgat ctccgtgaag ctggtttccg aaccgggcgt
    35881 tggcaccatc gccaccggtg tggcgaaagc ttatgccgat ttaatcacca tcgcaggcta
    35941 tgacggcggc accggcgcaa gtccgctttc atcggtgaaa tacgcaggct gtccgtggga
    36001 gctgggactt gttgaaacac aacaggcgct ggttgccaac ggcttgcgtc ataaaatccg
    36061 tttgcaggtc gatggcggcc tgaaaacggg tgtcgatatc atcaaagcgg cgattctcgg
    36121 cgcagaaagc ttcggcttcg gcactggccc aatggtcgcg ctcggctgta aatatctgcg
    36181 tatttgccac ctgaacaact gcgcaacggg tgtggcaact caggatgaca aactgcgtaa
    36241 gaatcactat cacggcctgc cgttcaaggt gacgaattac tttgagttta tcgcccgtga
    36301 aacccgcgag ctgatggcac agcttggcgt aacacgtctg gtggatctga ttggtcgcac
    36361 cgacctgctg aaagagctgg acggtttcac cgccaaacag cagaaactgg cgctgtcgaa
    36421 gctgctggag accgccgaac cgcatccagg taaggcactc tactgcaccg aaaccaaccc
    36481 gccgtttgat aacggcctgc tgaacgcgca gttgctgcaa caggcgaaac cgtttgtcga
    36541 tgagcgccag agcaaaacct tctggttcga tatccgcaac accgaccgct ctgtcggcgc
    36601 gtcgctttca ggctatatcg cccagacgca cggcgatcag gggctggcag ccgatccgat
    36661 taaagcgtac ttcaacggca ccgcaggcca gagcttcggc gtgtggaacg cgggcggcgt
    36721 ggaactgtac ctgaccggtg atgccaacga ctatgtcggt aaaggcatgg cgggcggctt
    36781 aatcgccatt cgtcctccgg ttggttccgc cttccgcagc catgaagcaa gtattatcgg
    36841 caacacctgc ctgtatggag cgacgggtgg tcgtctgtat gccgcaggcc gcgcgggtga
    36901 acgtttcggc gtgcgtaact ccggtgcaat caccgtggta gaaggcattg gcgacaacgg
    36961 ctgtgaatat atgacgggcg gtatcgtctg tattctgggt aaaaccggcg ttaacttcgg
    37021 tgcgggtatg accggcggct tcgcttacgt tctcgatgaa agcggcgatt tccgcaaacg
    37081 cgttaacccg gaactggtgg aagtattaag cgttgacgat ttggcgatcc atgaagagca
    37141 tctgcgtggt cttatcaccg agcatgtgca gcataccggt tcccagcgtg gtgaagagat
    37201 tctggcgaac tggtcaacct tcgccactaa atttgcgctg gttaaaccga agtccagtga
    37261 tgtcaaagca ctgctgggtc atcgtagtcg tagcgcagca gagttgcgcg tgcaggcgca
    37321 gtaaggggta gcaacaatga gtcagaatgt ttatcaattt atcgacctgc agcgcgttga
    37381 tccgccaaag aaaccgctga agatccgcaa aattgagttt gttgaaattt acgagccgtt
    37441 ttccgaaggc caggccaaag cgcaggctga ccgctgcctg tcgtgcggca acccatactg
    37501 cgagtggaaa tgcccggtac acaactacat cccgaactgg ctgaagctcg ccaacgaggg
    37561 gcgtattttt gaagcggcag aactgtcgca ccagaccaac actctgccgg aagtatgcgg
    37621 acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc accctgaacg atgagtttgg
    37681 cgcggtgacc atcggcaaca ttgagcgtta tatcaatgat aaagcgttcg aaatgggctg
    37741 gcgtccggat atgtccggcg tgaaacagac cggtaaaaaa gtggcgatta tcggcgcagg
    37801 tccggcaggt ctggcgtgtg cagatgtcct gacgcgcaac ggcgtacaag cggtcgtctt
    37861 cgaccgtcac ccggaaattg gcggcttgct gaccttcggt attccggctt tcaaactgga
    37921 aaaagaggta atgacgcgcc gccgtgaaat cttcaccggc atgggtattg aattcaaact
    37981 caataccgaa gtgggccgcg acgtacagct ggacgatctg ctgagtgatt acgatgccgt
    38041 gttccttggc gtcgggactt atcagtcaat gcgcggcggg ctggaaaacg aagacgccga
    38101 tggcgtgtac gcagcgctgc cgttccttat cgccaacacc aaacagttaa tgggctttgg
    38161 cgaaactcgt gaagaaccgt tcgtcagcat ggaaggcaaa cgcgtggtag tacttggcgg
    38221 tggcgatacc gcaatggact gcgtgcgcac gtctgtacgc cagggggcaa agcacgttac
    38281 ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc cgccgcgaag tgaaaaacgc
    38341 gcgggaagaa ggtgtagagt tcaaattcaa tgtccagcca ctgggaattg aagtgaacgg
    38401 taacggcaaa gtcagcggcg taaaaatggt gcgtactgaa atgggcgaac cggatgccaa
    38461 aggccgtcgc cgcgcggaga tcgtggcagg ttccgaacat atcgttccag cagatgcggt
    38521 gatcatggcg tttggtttcc gtccacacag catggaatgg ctggcaaaac acagcgtcga
    38581 gctggattcg caaggccgca tcatcgcccc ggaaggcagc gacaacgctt tccagaccag
    38641 caacccgaaa atctttgctg gcggcgatat cgtccgtggt tccgatctgg tcgttaccgc
    38701 tattgccgaa ggtcgtaagg cggcagacgg cattatgaac tggctggaag tttaagctct
    38761 gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa ttctcctttc gttctggaaa
    38821 gcgcctcgca taatctgaag aaaaccaatg taatacgcac atctggattg ttaccggcag
    38881 atgcccggcg tatagctgat tccattattc aacactgcgc catataaagg gaaagataat
    38941 ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa atccacgacg gtattattca
    39001 cctggtcgat agcgcccgga cggaaacggt acgtagcgtt aacgcgctga tgaccgcgac
    39061 atactgggaa attggccgac gaattgtcga atttgaacaa ggtggcgagg ccagggctgc
    39121 gtatggtgcg cagctaatca agcgactatc aaaggattta agtctaaggt ataagcgtgg
    39181 gttctctgca aaaaacttac gccaaatgag gcttttttac ctcttttttc aacatgttga
    39241 aattcgccag acagtgtctg gcgaattaac acctgccctg gtccacttac gtccgtttac
    39301 tttccgttaa aaacgctgac acccgcagct tttatgaaaa agagacgctc cgctgtggct
    39361 ggtctgttcg ccagctagaa cggcaaattg cgacccaatt ttatgagcga acactgctgt
    39421 cacatgacaa atcagccatg ctgcaacaac acgctcctgc cgagacgcat attcttccgc
    39481 aacaggcgat acgcgatccc tttgtgctcg aatttctgga attgaaagat gaatactcag
    39541 aatccgattt tgaggaggcg ctgatcaacc acctgatgga tttcatgctg gaacttgggg
    39601 atgattttgc ctttgttggt cggcagcgaa ggttacgcat tgatgacaac tggtttcgtg
    39661 tcgatctgct gtttttccac cgccgtttac gctgcctgct aatcgtcgat ctaaaagtgg
    39721 gcaaattcag ctatagcgat gccggacaga tgaatatgta tctcaactac gccaaagagc
    39781 actggacgct accggatgaa aatccgccca tcggtctggt tctctgtgca gagaaaggag
    39841 tcggagaagc gcattatgca ctggctggtt tgcctaacac cgttctggcg agtgaatata
    39901 agatgcaact acctgatgaa aaacgactcg cggatgaact cgttcgaaca caggcggtgc
    39961 tagaagaagg ctatagactc cgttaatttc agcggtttcg aattctgcag acactgtctg
    40021 cagaatcttt aaaacgaact aagttttaag ccattaaatc agccttaacc ttcaccacca
    40081 cctttttaat ctcaccaggt tcgccgacaa cgcatcctgg tttatgcggt tctcccggca
    40141 taaacacggc gaacattccc ggttttaagt tgatggtttg ctcgttttca atggcgctgc
    40201 aaagctggta atcatcttca tggtggaact cttcacactg gcgcgcagtg cctgccgtgc
    40261 caaacagaat ccgttcctca ccttttaata acagctggat atcaatgtat tgctcgtgca
    40321 attccgcttt tttctcgacg ggcgattgag tgttaaacgt catgacattc ataaaaatat
    40381 tgtcgccctg taattcgtaa cgacccggcg ctttttcttg cggtctggca gctaatgcca
    40441 gcgttaacgc gtcctgtaac gcaggatgta acccagcaga cggtaatgac tgtacttcac
    40501 ccatcatcat aatttttctc cctgggccaa cagcgcagcc ccaagtaaac ctgcatcatg
    40561 gcggtagtgc gccgccagta aatcaacatg aaatgccgct ggctcctgcg ccagatacgt
    40621 ttccaccagc gccagatacc cttctgccag accaacgctg ccaccgacca ccacgcactg
    40681 acaatcagtt gtggctttaa tatcagcgat cagccttgcc agcacatgtg cggagcggtg
    40741 aatcagctgc tgcgcctgct cgtcgccctg cccggcgcgc atgaaaatag ttttcgcatc
    40801 cgcgccagcc aactccccct gcgctgccgc tgcaataccg cgaccagagg caatcgcttc
    40861 cacgcaacct gtgcgtccac agccgcagac tgggccgtgt ggatcggcaa gcgtatgccc
    40921 gatgtgcccc gccagaccgc cagggccggt aagcagtttg ccgccgctca ctacaccgcc
    40981 gccgacgcca gtggaaacgg tgataaacac catatcggtt atatcgcctt ccagcgcctg
    41041 atactctgcc catgctgcgg cctgggcgtc gttaatggca atggtcggca aatcggtaag
    41101 ttgccccagc gttttgacta acggaaagtg tagcaatcca ccaagattat gcggattcag
    41161 cgccagcaaa ctgccgtcgc gaataattcc ggttgaagcg atggcaaccc gctgcgcatg
    41221 agcttgcaac ggagagacta atgcggataa ggcatcacgc aaggcttctg gtgtctggct
    41281 ggctggcgta ggaagttcac gacgatcgcg gatctgcccg tcagcgccaa tcagcgcggc
    41341 ggcaagttta gtaccgccga tatcaatcgc cagtgtggtc atagcaccgc ctttttcatc
    41401 gctgtgttgt accactgaca aatgtgttca agacgcgtaa tggcagaacc gaccgtcacc
    41461 gcccacgcgc cgtggcgcat cgcatcagcc gcctgagcag gcgtgttgta acgcccttcg
    41521 gcaatcaccc gacatccggc gtcgctcaac cttttcacca gcgtcagatc cggctcttct
    41581 ggcgtttcag gcgtggtata gccagaaagc gtagtgccaa taatttcggc tcccagcttt
    41641 tggcatgcca ggccgtcttc cggcgttgag cagtcggtca tcgccagtaa accgtgatga
    41701 tgaatacgtg ccagcagcgt ttcaacaggc accggacgcg ggcggtcggt gccgtcaatg
    41761 gcgataatgt ccgcgcccgc ctgcgccagc gcatcgacat cttcaatata ggccgtgatg
    41821 cgtaccggag aatcctccag atcgcgtttc acaattccaa taatcggcac gctcaccacc
    41881 gcacgcgtgg cttgcagatt tgccacacct tcgatgcgaa tggcaaccgc gcccgcctgc
    41941 tctgccgcta atgccatggc tgcgacgatt tcgggtttat cgagcgggct gtccggaacc
    42001 ggctggcagg agacaatcag gccaccgtta gcagcgattt tttgatccag ttgtgcaagt
    42061 aacgacatac atcttccctt agcgaaaggc ccgatacaga gaccgggcaa caggattaac
    42121 ttttggtttt gactaaatcg tttttggcgc tgccaaacgg cacggcaccg ctaaatggtt
    42181 tgccgtcgat agcgtcatga gtacgcaacg cttccgggcg cagccaacgc tgaacacgag
    42241 aaggcatatc cagaccaatc agcaggatca ccacaaatgt cagactgaac gagagcgatg
    42301 ccagtgcagt acccagatcc agacgttgag cgatcaacgc gccgatgatt ggggccagtg
    42361 cgccacccaa tgcgccaacg ttataggtaa agcccaggcc cgctgcacgc tggtcggtat
    42421 cgaaataacc gccaatcagt ttcggtaaga tcccggcgat cccttgtcca agcatttgct
    42481 ggaagaacag taacagaccg agcacccaga cgtttgcacc gccaatagca aataccggaa
    42541 taatcagcag ctgcgaggcc aacagactac aaacgtacgc tttgcgggtt cccagccagt
    42601 caccgaggaa gccaccgacg cagcacccca ccgccgcgcc aaagccacta aagaacagca
    42661 cattggcgac agtatgaggg ttataagcca gatcggtttt cagatacgtt ggcagcagcg
    42721 cctgaatcgg ccatgagtag aggaaagcaa acaacacgac caccatcagc attacgccag
    42781 ttggccagcg tttgcccgta ctctgcacca taaagctgat aaagattgcg gtgcataaca
    42841 gcccaagaac agcgacgatc gcggcatttt gcaggttacc ggcgaagcag aaccacagcg
    42901 cagtagccgc cgccagtgtc attacgatat tggcaatgcg atgttcacca cggtagagaa
    42961 tatccaccat tgtgcgtact ggtgctttac ctgcgtgttt ctctttccag tcttctgctt
    43021 ccgggatgtt tttacgcagc cagagagcaa agatgattgg caaaatgccg ataaagaaca
    43081 gcgcacgcca gccccagacc ggaaccacca ggctgtagac ctgagcggca acgacggccc
    43141 ctacagagaa gcctgaaatc agaaagccac tggctttgtt acgcagatgt tttggccagc
    43201 tttcaatgac ataggtggcg ctggaaccgt attcacccgc catccccatg ccgatgacca
    43261 gacgagcgat aaacatggtg atgtagcctg gcgcaaagcc gcatgccagc gtcccggccg
    43321 agaagagaac gatgctggtg accattgcca gacgacgccc gtagcggtca cccatagcgc
    43381 cgagcatcag gccgccgaac cagcgagaga taaaggctgc ggagatcaga cttgccgcct
    43441 gcaccgtcgt cagcccgaat tcaccttgta cttcggtgag taccagggcg attaaaacga
    43501 aatcaaaacc gtcaagcaga tatcccaacc aggcagcgga aaatgcccgc cattgtgcac
    43561 gattgagatg gcgataccac gggatattct gggttgtagt actcattgtg agtctcccgc
    43621 ggtgggcgat gcccacacgc tttggtatga aaattgtagg gtacagatgc gtttatttcc
    43681 cctcacccgg taggggcgag cgaggggaaa caactcaccc gcgctcttgc atcaactgct
    43741 gggccagcgc cttcagttct ggcagatatt tttcatctac cggaccaaac ggtttgcggc
    43801 acagcggcac agaaacgaca tccatataat gcagaacagt tttcaggccg cggaatacac
    43861 ccgttttgat cagtaaatca atgactttgt tgcattcagt ttgcagtttc tgcgcggtct
    43921 ggatatcgcc ttctttcagc gccttaacga tcccctgata gcgccagccc atgatgttgt
    43981 aggtactacc gataccacca tcagcgcccg ccagcaggcc agaggcgaag atttcgtcgt
    44041 aaccgttata gagcaccaga tcaggatgtt cacgacggat ctgctccatc tgatagagat
    44101 cgccagaggt ctgtttcagc gcacctacgc caggcaatgt aacaagtgtg ttgatctgat
    44161 ccagggtcag ttttacccca ctcagggctg gaatgttgta caccaccatc ggcaaaccat
    44221 ccgccgaatc aataattgcc cgatagtgat cgcagtgttc ttcaaagctg aaaggatagt
    44281 agaacggcgt gacggcggag acggcatcga agccataacg tttagccgat gccgcaagtt
    44341 gttggctttc ggcggtgctg acgcaaccga cgtgggcgat gagtttaatc ttacctttcg
    44401 cctcttcgga gacgatttcc agtacctgtt cacgctcgga aaggctttgt acaaaggctt
    44461 cgccggtcga accacccacg tataaaccgt caatgccctg ctgaatattg aactgaacca
    44521 ggcggcgcag actcgcttta tccagtgctt gttgttgatc aaaaggagtc aggagtgcag
    44581 ccattacgcc acgtaaattc gttgccataa atacctctga agtgatgctt gtctgataaa
    44641 cgatatacct ttatacctgt tataccagat caattaagca acaccccata cagaaagctt
    44701 ataatgcgat ctgcttcact aaagtggcat tatttctttt tgttggtggt ctgaccgaag
    44761 gcgtgccagg tagcagagac gctgttgaga tgcgattgca acgcacgatc ggcttcgtca
    44821 ggatcgtggc ggcggatcgc gtcaacgatc gcaatatgct gttgatagct aacgttgtta
    44881 tgctcgtgca gagcctgatc agccacggtt ggacgtgcgg cgatcagcca gtcgagcagg
    44941 gcaacgtgga tcgccatgaa gattgggtta ccggggatct ccgccagcac gcggtggaaa
    45001 tcaacgtctg agcgaatgaa tgccgcgttg ttatccagcg actgactgtt gatttccagt
    45061 gcttttgcca gcaaatcgat ttgctcatcg gtggcgtgtt cagccgcata gcgcaccagg
    45121 ctggattcaa agaacagacg taattgttcg aaatgggcaa tcccaccagg atgagaaagg
    45181 aaatctttcg ccatgccgga aagctcgccg atgatggtgt ccgcagaagg acgtgagacg
    45241 cgggcacgtt cgccgttgtt tatttgcacc agacctttgc gttttaacgc tgccagcgct
    45301 tcacgcaccg aaggacgccc gacgttaaag aacgccatca gttcgcgttc agacggtaat
    45361 tgttcacctt cgccaaattc acgacggcgg atcatctgtt ccagctcttc ttccaccatt
    45421 tcggagagtt ttttacgcgc cagcgggcgg ctacgcaagt tgcgaccaat tgcaggtgaa
    45481 gaatcttcgg tttgcgaatc aaatgcgttc ataaggccca ttctgtaagg tcagtgtgat
    45541 taacatcatc agtgacatcc tatcacagga ttgaaagtag gggaaaatgg cagggttttc
    45601 tctttgtgcc tcatcattac cataattaac ggaataatta actattgcga aaaattaatg
    45661 taacgcagat aaaaacatcc cgtttgaatt atttataaga ctattcacga gcattatgaa
    45721 tattatgaat gtgttcttac aaaataatca taagcgcata ttttttaatg aaaaatcacc
    45781 tcacctacaa ttaaaaacac gacatccgca ccataaatag acttgcaaaa aatataacat
    45841 cattgttttc aatctgccgt ttatgggatt gacccttttc ttttgacacg gagttcaaca
    45901 atgttcggca taattatatc tgtcatcgta ttaattacga tgggctattt gatcctgaaa
    45961 aactacaaac ctcaggtggt gctggctgca ggtatcttcc tgatgatgtg cggtgtctgg
    46021 ttagggttag gtggtgtact cgatcccgcc aaaagcagcg gctacttgat cgtcgatatt
    46081 tataatgaaa tcctgcgcat gctgtccaac cgcattgccg gattggggct gtcgattatg
    46141 gcggtgggcg gttatgcccg ctacatggag cgcacaggag ccagtcgcgc gatggtgagc
    46201 ttgttaagcc gcccgttaaa actcattcgc tcgccgtata ttattctgtc ggcaacttac
    46261 gtcatcggcc aaatcatggc gcagtttatt accagcgcct ccggcctggg tatgttgctg
    46321 atggtcacct tatttccgac actggtgagt ctgggagtaa gccgtctctc tgcggtggcg
    46381 gttatcgcaa ccacaatgtc cattgagtgg gggattctgg aaacgaactc catttttgca
    46441 gcccaggtag cgggaatgaa aattgccact tacttcttcc actaccagct tccggtcgcc
    46501 tcttgcgtca ttatctcggt ggcgatctcc cactttttcg tgcaacgcgc ttttgacaaa
    46561 aaagataaaa atatcaatca cgaacaggca gagcaaaaag ctctcgataa tgtcccgccg
    46621 ctctattacg ccattttacc tgtgatgccg ttaatcctga tgctcggctc gctattcctt
    46681 gcccacatcg ggctgatgca gtcagaactg catctggtgg tggtgatgtt actgagtttg
    46741 acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga cgatgtgcag
    46801 gcgttttttg acggcatggg tacgcagttt gccaacgtgg taacgctggt ggtcgcgggt
    46861 gaaatatttg cgaaaggctt aacgacgatt ggcactgtcg atgcggttat caggggtgcg
    46921 gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctggt cattgccatt
    46981 tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag tcttattccg
    47041 aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat gcattttgcc
    47101 acgacgctag cgcgcggttt cgccgattac tgcggtggtg gtcgttacgt caggaattgc
    47161 aggcgtttcg ccttttgcgg tggtgaagcg gacagcgatc cccatggcag tcggtttcgt
    47221 ggtgaatatg attgccacaa tcacgctatt ttattaagtc attaaaagac aaaacaggcc
    47281 gccagggcct gtttttgtat tacttcacaa cgcgtaatgc cggtcgacca ccgcgtggtg
    47341 gctgcggtgg ttcatcgtca ggatgagtgt catcatcgtg atctggcttg tcgccatcaa
    47401 taaccgacat aacggtttcg ttgtctgccg atgcctcttc atcattcatg atgctggtat
    47461 cttcatcgta ggcagcttca ggctcaaaca tcgtgcctgc gccattttca cgggcgtaga
    47521 tagccagcac ggcagccagc ggcacagaaa cctgacgcgg aatgccacca aagcgcgcgt
    47581 taaagcgcac ctcatcattc gccagttcca gattgccgac agcacgcggc gcaatgttga
    47641 gtacgatttg cccgtcacgc gcatattcca taggaacctg cacgccaggg agcgtcacat
    47701 ccaccaccag gtgcggcgtg agctggttat ccagcaacca ctcatagaat gcacgcagca
    47761 gatagggacg acgtggtgtt agctgtgaca aatccataca gattaactcc ggcccagacg
    47821 catttcacgt tctgcttcag ttaaagaagc aaggaaagag tcacgctcaa agacgcgggt
    47881 catatagcct ttcagctctt tcgcacccgg gccgctgaac tcgataccca gttgcggcag
    47941 acgccacagc agcggagcaa ggtagcaatc gaccaggctg aactcatcgc tcaggaagta
    48001 cggcttctga ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt gcttacgtgc
    48061 ggcatctgct tcagaagctg aaccgttgat gatggtgttc atcagcgtgt accagtcttt
    48121 ttcgatgcga tgcatgtaca aacggctttc accgcgagct accgggtaaa caggcatcag
    48181 tggcggatgc gggaaacgct catccagata ttccataatg atgcgagatt cccacagggt
    48241 cagctcacga tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa tcagatcctg
    48301 aggcggattg tccttttcca cgtgttcgat ctcgaaactt acacctttct cagccagcac
    48361 aatgcggacc tgatggctat agatgtcagt aggaccggaa aacagcgtca ttaccgaacg
    48421 tttgttggca gcgacagcca tgaaaacctc caggtatagt cagaattttt actgctacca
    48481 gccaccaggt ggccagtcag aagttgtgtt acccagtaag gaacgactct ctttgttcga
    48541 aaatcaaaca aaaaatgagc aatacccgac atttgggcag aaaattggat gatagtttac
    48601 cagattttgc gacctttgtg gtgagtcgat gccggaaatg gggaaaaaga gatgcgcttt
    48661 agtctgaaat agttgactta gtcccttatt ggcgatgtgg tttttgtttt gcctgtctgt
    48721 cagacggcag caaaaagcaa ctttccattt tttacgctga ttcagaattt aaccataaaa
    48781 aaacccgccg aagcgggttt tttcgcaaat tgttttctgc cggagcagaa gccaattaac
    48841 gtttggagaa ctgcggacga cgacgtgctt tacgcagacc gactttctta cgttcaacct
    48901 gacgagcgtc acgagtaacg aagccagctt tacgcagttc agaacgcagg gactcgtcgt
    48961 attccatcag agcgcgggtg ataccgtgac ggatcgcacc agcctgacca gagataccac
    49021 cacctttaac ggtgatgtac aggtccagtt tctcaaccat atcgaccagt tccagcggct
    49081 gacgaactac catgcgagca gtttcacgac cgaagtactg ttccagagaa cgttggttga
    49141 ttacgatttt accgttgccc ggtttgatga aaacgcgagc tgcggaactt ttgcggcgac
    49201 cagtgccgta gtattgattt tcagccattg cctataatcc cgattagatg tcaagaactt
    49261 gcggttgctg tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt ttacggaaca
    49321 tagcacgacc cagcgggcct tttggcaaca tgcctttaac cgcgatttca atcacacgct
    49381 caggacggcg agcaatcatc tcttcaaagg tcgcttgttt gataccaccg atgtggccgg
    49441 tgtggtgata gtacactttg tcagtacgct tgttgccggt tacagcaact ttgtcagcgt
    49501 tcagaacgat gatgtaatca ccggtatcta cgtgcggagt gtattccgct ttgtgcttac
    49561 cgcgcaggcg acgagccagt tcagtagcca gacggcccag agttttaccg gtcgcgtcaa
    49621 caacatacca gtcgcgtttt acggtttctg gtttagctgt aaaagttttc attaaaagct
    49681 tacccaataa atagttacac gttggtgaac acccaaacgt cttcaattgt tgaggttcac
    49741 acgacaaagt ccggcaaacc taccccttcg aatagcctat gccagcacac aaaaagtttt
    49801 gggaaaaaaa ctttcttgta acgtggggtc gcaggattat agagaagtcg gggtcaaaga
    49861 tcgacccctt tttgtgattt gtggcaggtt ttaacccgct aaatgctcgc gcttcagata
    49921 ctcttcgctt tgcatctctt gcagacgtga caggcaacgc tggaactcaa acttcagccg
    49981 atcgccctga taaatttcat acagcggcac ttctgcactc accactaatt tgacatggcg
    50041 ctcgtaaaac tcatccacca gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt
    50101 cataactggt acatcaaaca acatgaccgt atgaaagaga cgtgagagcg caatatagtc
    50161 atgctgactg cgggcgtcga cgcacagcgt agtaaaagag accgccagcg tctggttctc
    50221 gacgcccatt gtcgctaatg gccgatggtt gatttctaac gtcggtgaat tttctcgttt
    50281 cccccccgcc agcgccaacc atagtttatc catttgcgcc cgggtttcat cgtggagtgg
    50341 cgaaagccac agatgcgcct gagtgagtgt acgcagacga taatcaacac cagcgtccac
    50401 gttcattaca tcacaatgct gtttaatggc atcgattgca ggcagaaaac gcgcacgttg
    50461 caagccattt cgataaagtt cgtccggcgg aatatttgac gtcgctacca gggtaatacc
    50521 gcgggcgaac agggctttca tcaggccgcc aagtagcatg gcatcggtaa tatcagagac
    50581 aaaaaattcg tcaaaacaga gcacgtcagt ttctgcttta aaacgatcag caataatttc
    50641 cagcggatcg gtctgcccct gtaaggcagt tagctcttca tgcacccgca gcataaaacg
    50701 gtgaaagtgc aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa ggtccatcag
    50761 ccaggttttc ccgcgtccta caccgcccca catatataag ccacgcactg gcgtatgctt
    50821 tgtgtcttcc cgtttacccc acagcttacc gacccgcgcc attagcccac tcgtcctggg
    50881 ggctggtggc gtgctattga tgagttcctg ataaataatt tccaagcggc tgacggcctc
    50941 tttttgaacg tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt attgcgatgt
    51001 tggagtaacg ctttgcatga tcttattgtt attccttgaa taatcggtgc gccgttgttc
    51061 acggttgacg aaaaaaaggc cgttctacac tacgcgatat gcagtcggga ttccacttct
    51121 gtggaattaa cggttatagt ggcataatca gccgcaggca tggagcctga agccaacacc
    51181 ctacggaaac aaaagacaac gggagatgtt catgacctgg gaatatgcgc taattgggtt
    51241 agtcgtcggc atcattattg gtgctgtggc catgcgtttt ggtaatcgta aactacgcca
    51301 gcaacaggcg ttgcagtacg aactggaaaa gaataaagct gaactggacg agtatcgcga
    51361 agagctggtt agccactttg cccgcagcgc ggaattactg gataccatgg cgcacgacta
    51421 tcgccagctg tatcagcaca tggcgaaaag ctccagcagc ctgctgccgg aactgtctgc
    51481 tgaagcaaac ccgttccgta atcgtctggc agagtctgaa gccagcaacg atcaggcacc
    51541 ggtgcagatg cctcgcgact attctgaagg cgcatccggc ctgctgcgta ctggcgcgaa
    51601 gcgcgactaa tttatttttc gggcgcagcc attgcgccct cctcttctct ccctccccga
    51661 ctatcattta atctggtgtc tcattgttag ccgtctgaaa attcaataac atcaaactgt
    51721 tttgaatctc ttttcttatc attcaggtac gagagcagga ataatgaaaa aacaaaccca
    51781 gctgttgagt gcattagcgt taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt
    51841 tgcgtcgatc ccaggccagg ttgccgatca ggcccccctc cccagtctgg ctccaatgct
    51901 ggaaaaagtg cttccggcag tggtgagcgt acgggtggaa ggaacggcca gtcagggaca
    51961 gaaaatcccg gaagaattca aaaagttttt tggtgatgat ttaccggatc aacctgcaca
    52021 acccttcgaa ggtttaggct ccggtgtcat catcaacgcc agtaaaggct atgtgctgac
    52081 caacaaccat gtgattaatc aggcacagaa aatcagtatt cagctcaatg atgggcgcga
    52141 gtttgatgca aaactgattg gtagcgatga ccagagcgat atcgccctgt tgcaaattca
    52201 aaacccgagc aaattaacgc aaatcgctat tgccgactcc gataaattgc gcgtcggtga
    52261 ttttgccgtg gcggtcggta acccatttgg ccttgggcaa accgccacct ctggcattgt
    52321 ttccgcatta ggacgcagcg ggttgaatct tgaaggtctg gaaaacttta tccagacaga
    52381 tgcttccatt aaccgcggta actccggcgg tgcactgtta aaccttaacg gtgagttaat
    52441 tggcatcaac actgcaatcc ttgcgcctgg cggcgggagc gtcggaattg gatttgccat
    52501 ccccagtaat atggcgcgaa cactggcgca gcagcttatc gactttggtg aaatcaaacg
    52561 cggtttgtta ggcatcaaag gcaccgagat gagtgccgat atcgccaaag ccttcaacct
    52621 tgacgtgcag cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct cggcgaaagc
    52681 gggcgtcaaa acgggcgata ttattaccag cctcaacggc aaaccgctga atagctttgc
    52741 tgagttgcgc tctcgtatcg cgaccaccga gccgggcacg aaagtgaagc ttggcctgct
    52801 gcgtaacggc aaaccactgg aagtagaagt gacgctcgat accagcacct cttcgtcggc
    52861 cagcgctgaa atgatcacgc cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa
    52921 agatggcggc aaaggtatta aaatcgatga agttgtcaaa ggaagcccag ctgctcaggc
    52981 tggcttgcaa aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga actcgattgc
    53041 tgaaatgcgt aaagtgctgg cggcaaaacc ggccatcatc gccctgcaaa ttgtacgcgg
    53101 caatgaaagc atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca ggctgacgtg
    53161 tgatgtccgg ttaactcgtg gtatgctgct gccgttccct tttttaatga cgcctccatc
    53221 atgtttgtga agctcttacg ttccgttgcg attggattaa ttgtcggcgc tattctgctg
    53281 gttgccatgc cttcgctgcg cagccttaac ccgctttcca ctccgcaatt tgacagtacc
    53341 gatgagacgc ctgccagtta taatctggcg gttcgccgcg ccgcgccagc ggtggttaac
    53401 gtttacaacc gtggtttgaa caccaactct cacaaccagc ttgagatccg caccctggga
    53461 tccggtgtaa tcatggatca acgcggttat atcatcacca ataaacacgt catcaacgac
    53521 gccgatcaga tcatcgtcgc cttacaggat ggacgtgtat ttgaagcatt gctggtggga
    53581 tctgactctc taaccgatct ggcggtactt aaaattaatg ccactggcgg tttacctacc
    53641 attccaatta atgcacgtcg cgtaccgcac attggcgacg tggtactggc gatcggtaac
    53701 ccgtacaacc tcgggcagac cattacccag gggattatta gtgctacggg tcgaatcggt
    53761 ctgaacccga ccgggcggca aaacttccta caaaccgatg cttccattaa ccacggtaac
    53821 tctggcggcg cgctggtgaa ctcgctgggc gaactgatgg gcattaacac gctgtcgttt
    53881 gataagagta acgatggcga aacgccggaa ggtatcggct ttgcgattcc tttccagtta
    53941 gcaaccaaaa ttatggataa gctgatccgc gatggtcgcg tgatccgcgg ctacattggt
    54001 atcggcggac gcgagatcgc accactgcac gcgcagggcg gtggtataga tcaactgcaa
    54061 gggatcgtgg ttaatgaagt gtcacctgac ggcccggcgg cgaatgcggg tattcaggtc
    54121 aacgatctga ttatttcggt ggataacaaa ccggccatct ctgctctgga gacgatggat
    54181 caggtggcag aaattcgccc tggttcggtg atcccggttg tagtgatgcg tgatgataag
    54241 cagttaacgc tgcaggtcac cattcaggaa tatccggcaa ccaattaagg ccagcgtgaa
    54301 agacaaaaaa accggagtct gtgctccggt tttttattat ccgctaatca attacttatt
    54361 aacgaactct tcgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc
    54421 gttctgttca aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc
    54481 cagcagaagc ggctgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca
    54541 ttcgacaacg ccttgttcgc cctgcagtgc gcgaaccaga gacaggccaa aacgtgcagc
    54601 agcctggccc atagacaggg ttgccgaccc gccaccggct ttcgcttcaa ccacctcagt
    54661 acccgcgttc tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc
    54721 aggaacctgt gacaacagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac
    54781 ttcaacttcg cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat
    54841 atccagcgtg gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac
    54901 ttcagcagca atcgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt
    54961 cgggcaggtt ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacattaaa
    55021 caggtcggaa cgatccatac ccggtttacg cgctacacct gcagagataa gaacgacatc
    55081 tgcgccttcc agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt
    55141 agggatatgg ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga
    55201 gagttctgaa cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc
    55261 gccagcagcg ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa
    55321 gatatgttgc tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat
    55381 ctcaagaatg tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa
    55441 cctaagttac gtaattaaca atcgcttatg cattagcgca acattccaac aggtggtgac
    55501 aatataccct accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa
    55561 gcagaatttg catgccgtga cgcaggcata tttctcaata acgaaatttg ataaaatccc
    55621 gctctttcat aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc
    55681 tgtatgcaca ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta
    55741 agcaagaaga actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc
    55801 agggcgaaat cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag
    55861 tctcgcggat gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg
    55921 tttactgcct gccagctgaa ctgggtgtac caaccacctc cagtccattg aaaaatctgg
    55981 tgctggatat cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc
    56041 agttaattgc tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg
    56101 ctggcgatga caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtatg
    56161 aagcgatttt agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc
    56221 ggggaaaatg ttgcttatcc ctctcaaccc cctgctttcc cctgcgatta atttaacgaa
    56281 tagtgcgttt tactgcgaca tgtcattcac acaat
//
LOCUS       DAZFBR010000018        53157 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFBR010000018 DAZFBR010000000
VERSION     DAZFBR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.018"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            184..336
                     /gene="mokC"
                     /locus_tag="ACHJZ2_001252"
     CDS             184..336
                     /gene="mokC"
                     /locus_tag="ACHJZ2_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO4567404.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(440..1570)
                     /gene="dnaJ"
                     /locus_tag="ACHJZ2_001253"
     CDS             complement(440..1570)
                     /gene="dnaJ"
                     /locus_tag="ACHJZ2_001253"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO4567405.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1659..3575)
                     /gene="dnaK"
                     /locus_tag="ACHJZ2_001254"
     CDS             complement(1659..3575)
                     /gene="dnaK"
                     /locus_tag="ACHJZ2_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO4567406.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3952..4356
                     /gene="yaaI"
                     /locus_tag="ACHJZ2_001255"
     CDS             3952..4356
                     /gene="yaaI"
                     /locus_tag="ACHJZ2_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO4567407.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4382..5095
                     /gene="msyB"
                     /locus_tag="ACHJZ2_001256"
     CDS             4382..5095
                     /gene="msyB"
                     /locus_tag="ACHJZ2_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4567408.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5244..5810
                     /gene="satP"
                     /locus_tag="ACHJZ2_001257"
     CDS             5244..5810
                     /gene="satP"
                     /locus_tag="ACHJZ2_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO4567409.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5845..6432)
                     /gene="mog"
                     /locus_tag="ACHJZ2_001258"
     CDS             complement(5845..6432)
                     /gene="mog"
                     /locus_tag="ACHJZ2_001258"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO4567410.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6547..7500)
                     /gene="tal"
                     /locus_tag="ACHJZ2_001259"
     CDS             complement(6547..7500)
                     /gene="tal"
                     /locus_tag="ACHJZ2_001259"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4567411.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7779..9209
                     /gene="yaaJ"
                     /locus_tag="ACHJZ2_001260"
     CDS             7779..9209
                     /gene="yaaJ"
                     /locus_tag="ACHJZ2_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO4567412.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            9279..10055
                     /gene="yaaA"
                     /locus_tag="ACHJZ2_001261"
     CDS             9279..10055
                     /gene="yaaA"
                     /locus_tag="ACHJZ2_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO4567413.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10208..10504)
                     /gene="yaaX"
                     /locus_tag="ACHJZ2_001262"
     CDS             complement(10208..10504)
                     /gene="yaaX"
                     /locus_tag="ACHJZ2_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO4567414.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10718..12004)
                     /gene="thrC"
                     /locus_tag="ACHJZ2_001263"
     CDS             complement(10718..12004)
                     /gene="thrC"
                     /locus_tag="ACHJZ2_001263"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO4567415.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12005..12937)
                     /gene="thrB"
                     /locus_tag="ACHJZ2_001264"
     CDS             complement(12005..12937)
                     /gene="thrB"
                     /locus_tag="ACHJZ2_001264"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO4567416.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12939..15401)
                     /gene="thrA"
                     /locus_tag="ACHJZ2_001265"
     CDS             complement(12939..15401)
                     /gene="thrA"
                     /locus_tag="ACHJZ2_001265"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO4567417.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15427..15540)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15482..15565)
                     /locus_tag="ACHJZ2_001266"
     CDS             complement(15482..15565)
                     /locus_tag="ACHJZ2_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO4567418.1"
                     /translation="MKRISTTITTTITTTITITITTGNGAG"
     gene            complement(15779..16465)
                     /gene="yjtD"
                     /locus_tag="ACHJZ2_001267"
     CDS             complement(15779..16465)
                     /gene="yjtD"
                     /locus_tag="ACHJZ2_001267"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4567419.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16865..17005)
                     /gene="yjjY"
                     /locus_tag="ACHJZ2_001268"
     CDS             complement(16865..17005)
                     /gene="yjjY"
                     /locus_tag="ACHJZ2_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO4567420.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17101..17817
                     /gene="arcA"
                     /locus_tag="ACHJZ2_001269"
     CDS             17101..17817
                     /gene="arcA"
                     /locus_tag="ACHJZ2_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO4567421.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17877..19229)
                     /gene="creD"
                     /locus_tag="ACHJZ2_001270"
     CDS             complement(17877..19229)
                     /gene="creD"
                     /locus_tag="ACHJZ2_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO4567422.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGIGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19287..20711)
                     /gene="creC"
                     /locus_tag="ACHJZ2_001271"
     CDS             complement(19287..20711)
                     /gene="creC"
                     /locus_tag="ACHJZ2_001271"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO4567423.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20711..21400)
                     /gene="creB"
                     /locus_tag="ACHJZ2_001272"
     CDS             complement(20711..21400)
                     /gene="creB"
                     /locus_tag="ACHJZ2_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO4567424.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(21413..21886)
                     /gene="creA"
                     /locus_tag="ACHJZ2_001273"
     CDS             complement(21413..21886)
                     /gene="creA"
                     /locus_tag="ACHJZ2_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO4567425.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22097..22966
                     /gene="robA"
                     /locus_tag="ACHJZ2_001274"
     CDS             22097..22966
                     /gene="robA"
                     /locus_tag="ACHJZ2_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO4567426.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22963..23610)
                     /gene="gpmB"
                     /locus_tag="ACHJZ2_001275"
     CDS             complement(22963..23610)
                     /gene="gpmB"
                     /locus_tag="ACHJZ2_001275"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO4567427.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23662..24174
                     /gene="yjjX"
                     /locus_tag="ACHJZ2_001276"
     CDS             23662..24174
                     /gene="yjjX"
                     /locus_tag="ACHJZ2_001276"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO4567428.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(24321..24647)
                     /gene="trpR"
                     /locus_tag="ACHJZ2_001277"
     CDS             complement(24321..24647)
                     /gene="trpR"
                     /locus_tag="ACHJZ2_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO4567429.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24737..26674)
                     /gene="sltY"
                     /locus_tag="ACHJZ2_001278"
     CDS             complement(24737..26674)
                     /gene="sltY"
                     /locus_tag="ACHJZ2_001278"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO4567430.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26885..28552
                     /gene="ettA"
                     /locus_tag="ACHJZ2_001279"
     CDS             26885..28552
                     /gene="ettA"
                     /locus_tag="ACHJZ2_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO4567431.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28659..28826)
                     /gene="nadS"
                     /locus_tag="ACHJZ2_001280"
     CDS             complement(28659..28826)
                     /gene="nadS"
                     /locus_tag="ACHJZ2_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO4567432.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(28860..30092)
                     /gene="nadR"
                     /locus_tag="ACHJZ2_001281"
     CDS             complement(28860..30092)
                     /gene="nadR"
                     /locus_tag="ACHJZ2_001281"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO4567433.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30113..31495)
                     /gene="radA"
                     /locus_tag="ACHJZ2_001282"
     CDS             complement(30113..31495)
                     /gene="radA"
                     /locus_tag="ACHJZ2_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO4567434.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31544..32512)
                     /gene="serB"
                     /locus_tag="ACHJZ2_001283"
     CDS             complement(31544..32512)
                     /gene="serB"
                     /locus_tag="ACHJZ2_001283"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO4567435.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32618..33259
                     /gene="ytjB"
                     /locus_tag="ACHJZ2_001284"
                     /pseudo
     CDS             32618..33259
                     /gene="ytjB"
                     /locus_tag="ACHJZ2_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
     gene            33290..34306
                     /gene="lplA"
                     /locus_tag="ACHJZ2_001285"
     CDS             33290..34306
                     /gene="lplA"
                     /locus_tag="ACHJZ2_001285"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO4567436.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34338..34601
                     /locus_tag="ACHJZ2_001286"
     CDS             34338..34601
                     /locus_tag="ACHJZ2_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4567437.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(34762..35481)
                     /gene="deoD"
                     /locus_tag="ACHJZ2_001287"
     CDS             complement(34762..35481)
                     /gene="deoD"
                     /locus_tag="ACHJZ2_001287"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO4567438.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35538..36761)
                     /gene="deoB"
                     /locus_tag="ACHJZ2_001288"
     CDS             complement(35538..36761)
                     /gene="deoB"
                     /locus_tag="ACHJZ2_001288"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO4567439.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36813..38135)
                     /gene="deoA"
                     /locus_tag="ACHJZ2_001289"
     CDS             complement(36813..38135)
                     /gene="deoA"
                     /locus_tag="ACHJZ2_001289"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO4567440.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38262..39041)
                     /gene="deoC"
                     /locus_tag="ACHJZ2_001290"
     CDS             complement(38262..39041)
                     /gene="deoC"
                     /locus_tag="ACHJZ2_001290"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO4567441.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39299..40848
                     /gene="yjjI"
                     /locus_tag="ACHJZ2_001291"
                     /pseudo
     CDS             39299..40848
                     /gene="yjjI"
                     /locus_tag="ACHJZ2_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            40820..41683
                     /gene="yjjW"
                     /locus_tag="ACHJZ2_001292"
     CDS             40820..41683
                     /gene="yjjW"
                     /locus_tag="ACHJZ2_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO4567442.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(41897..42679)
                     /gene="yjjV"
                     /locus_tag="ACHJZ2_001293"
     CDS             complement(41897..42679)
                     /gene="yjjV"
                     /locus_tag="ACHJZ2_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4567443.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(42676..43749)
                     /gene="yjjU"
                     /locus_tag="ACHJZ2_001294"
     CDS             complement(42676..43749)
                     /gene="yjjU"
                     /locus_tag="ACHJZ2_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO4567444.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(43871..44032)
                     /gene="ytjA"
                     /locus_tag="ACHJZ2_001295"
     CDS             complement(43871..44032)
                     /gene="ytjA"
                     /locus_tag="ACHJZ2_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO4567445.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44159..44764)
                     /gene="osmY"
                     /locus_tag="ACHJZ2_001296"
     CDS             complement(44159..44764)
                     /gene="osmY"
                     /locus_tag="ACHJZ2_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO4567446.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45157..46746)
                     /gene="prfC"
                     /locus_tag="ACHJZ2_001297"
     CDS             complement(45157..46746)
                     /gene="prfC"
                     /locus_tag="ACHJZ2_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO4567447.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(46837..47514)
                     /gene="yjjG"
                     /locus_tag="ACHJZ2_001298"
     CDS             complement(46837..47514)
                     /gene="yjjG"
                     /locus_tag="ACHJZ2_001298"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO4567448.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(47529..47975)
                     /gene="rimI"
                     /locus_tag="ACHJZ2_001299"
     CDS             complement(47529..47975)
                     /gene="rimI"
                     /locus_tag="ACHJZ2_001299"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4567449.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(47944..48357)
                     /gene="holD"
                     /locus_tag="ACHJZ2_001300"
     CDS             complement(47944..48357)
                     /gene="holD"
                     /locus_tag="ACHJZ2_001300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO4567450.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            48460..49491
                     /gene="rsmC"
                     /locus_tag="ACHJZ2_001301"
     CDS             48460..49491
                     /gene="rsmC"
                     /locus_tag="ACHJZ2_001301"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO4567451.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            49681..49767
                     /locus_tag="ACHJZ2_001302"
     tRNA            49681..49767
                     /locus_tag="ACHJZ2_001302"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49715..49717,aa:Leu,seq:cag)
     gene            49810..49886
                     /locus_tag="ACHJZ2_001303"
     tRNA            49810..49886
                     /locus_tag="ACHJZ2_001303"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49844..49846,aa:Pro,seq:tgg)
     gene            50033..51268
                     /gene="aslB"
                     /locus_tag="ACHJZ2_001304"
     CDS             50033..51268
                     /gene="aslB"
                     /locus_tag="ACHJZ2_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO4567452.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(51436..53091)
                     /gene="aslA"
                     /locus_tag="ACHJZ2_001305"
     CDS             complement(51436..53091)
                     /gene="aslA"
                     /locus_tag="ACHJZ2_001305"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO4567453.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
BASE COUNT        12441 a        14358 c        13514 g        12844 t
ORIGIN      
        1 agaatgggtt aaagagagcg atataatagc ggcgggtgct tgaggctgtc tgtctcaggc
       61 attagctgaa cggcagatag agaaaagccc cgagtgatat tttaccatca acccgaggcc
      121 ccctatatgc tgaacacatg tagagtgcct cttactgacc gtaaggtcaa ggagaagaga
      181 gcaatgaagc agcataaggt gatgattgtc gccctgatcg tcatctgtat caccgccgta
      241 gtggcggcgc tggtaacgag aaaagacctc tgtgaggttc acatccgaac tggccagacg
      301 gaggttgctg ttttcacggc ttacgaatcc gagtaagagc aacggcgggg agtgatcccc
      361 gccactttac aggtgctcgc atatcttcaa cgcaccctat ttttacccag gcctgcccac
      421 gggcaggctt ttggggaggt tagcgggtca ggtcgtcaaa aaacttcttc acaccatcaa
      481 agaagctctt tgagcgcggg ctgttgtgct cgccggttgg gccaccgaag ctttcttgca
      541 gctcttgcag cagctgtttc tgcttctcgt tcaggcctac cggtgtttcg acgacaacgc
      601 ggcacagcaa atcaccctgt gcgccaccgc ggacagactt gacgccttta ccgcgcatac
      661 ggaatagctt accggtctgg gtttcgccag gcactttcag tttgacgcga ccatcaaggg
      721 tcggtacttc gatttcacca cccagcgccg ccatagcgaa gttgatcggg acttcgcaat
      781 acaggttgtt gccttcacgc tcgaaaatcg ggtgctgttt aacctgaacc tgaacgtaca
      841 gatcgcctgc cggtgcgccg tgttcacccg cttcaccttc gcccgcaaga cggatgcggt
      901 ctccagtgtc cacccctgcc gggattttaa cggacagcgt tttgctgcgc tcaacacgac
      961 catgaccatg acatttgttg cacggatctt tgatcagcgt accgcggccc tgacagtgtg
     1021 gacaggtctg ctgcacggca aagaaaccct ggcgcatctg cacctgacca gaaccatgac
     1081 aggtcggaca ggtctgcggc tgtgtacctg gttttgcacc gctaccgtgg caaacgtcac
     1141 actcttccag agtcggaatg cggatctctt tggtcacgcc acgtacagct tcttcgaggg
     1201 tgagttccat gttatagcgt aaatcagcac cgcgcgccgc acgttgacga ccacgtccgc
     1261 cgccaaaaat atcgccgaaa acgtcaccaa aaatatcgct gaagtctgcg ccgccgccaa
     1321 aaccgccgcc gcccatgcca ccttgctcaa acgcagcatg accatactga tcgtatgccg
     1381 cacgtttttg cgagtcggtc agaacttcat aagcttcctt gatctcttta aatttcgcct
     1441 cggcctcttt gtcaccctgg ttacggtccg ggtggtattt catggccagg cgtttgtagg
     1501 cctttttgat ttcacgctct tccgctgttt tggaaacgcc taaaatctcg taataatctt
     1561 gcttagccat cttttttaaa ttgcccctag atgaatgcac gggcggagag gaaattcccc
     1621 ttcgcccgtg tcagtataat tacccgttta tagggcgatt attttttgtc tttgacttct
     1681 tcaaattcag cgtcgacaac atcgtcatct ttcgcgttgt ttgcagaagc atcagcaccg
     1741 gcagtctgct gctgggcatg ttgctgctgg gcgatttcca tcagtttctg ggaaacctgt
     1801 gccagttcct gcattttcgc ttcgatagcg gctttgtctt cacctttcag agcagtttcc
     1861 agtgcagtca gcgcagactc gatagcagtt ttgtcgtcag ccggcagttt gtcgcctgct
     1921 tcttcaacct gcttacgggt gctgtgcagc agatggtcgc cctggttgcg agtctgtacc
     1981 agctcttcaa acttacggtc agcttcggcg ttagcttcgg cgtcgcgtac cattttctgg
     2041 atttcatctt cgttcagacc agaagacgcc ttgatggtga tcttctgctc tttaccgctg
     2101 tttttatctt tcgcggaaac gtgcaggata ccgtcagcat cgatatcgaa ggtaacttcg
     2161 atctgcggca tgccgcgcgg tgccgggttg ataccatcca ggttgaactg acccagagat
     2221 ttgttgtcag ccgcacgttt acgttcaccc tgcagcacat ggatggttac cgcagactgg
     2281 ttgtcttcag cggtagagaa cacctggctg tgcttggtcg ggatagtggt gtttttcgcg
     2341 atcagcgtcg tcatcacacc gcccatggtt tcgataccca gagacagcgg ggtaacatcc
     2401 agcagcagta cgtcttttac gtcaccagtc agaacaccac cctgaacagc agcaccgatt
     2461 gctacagctt cgtccgggtt aacgtcttta cgcggctctt taccaaagaa ctcagcaact
     2521 ttcttctgaa ccattggcat acgagtctga ccaccaacga ggataacgtc gtcgatatca
     2581 gatacggaca ggccagcgtc ctgcagcgca actttcagcg gctcgatgga acggttaacc
     2641 agatcttcaa ccaggctttc cagtttcgca cgagtcactt tgatgttcat gtgtttcgga
     2701 ccggtcgcgt ctgcagtgat gtacggcagg ttaacgtcgg tctgctgagc ggaagacagt
     2761 tcgattttcg ctttttctgc cgcttctttc aggcgctgca ttgccagcgg atcgttgcgc
     2821 aggtcaatgc cctgatcttt cttgaattct tcaaccagat agttgatcag acggctgtcg
     2881 aagtcttcac cacccaggtg ggtatcaccg ttggttgcca gaacttcgaa ggttttttcg
     2941 ccgtcaactt cgtcgatttc gataatagaa atatcgaaag taccaccacc caagtcataa
     3001 accgcgatag tacggttgcc agtacctttg tccagaccgt aagccagcgc agctgcggtc
     3061 ggttcgttga tgatacgttt tacttccaga ccagcgatac ggcctgcgtc tttggttgcc
     3121 tgacgctgag catcgttaaa gtatgccggt acggtgataa cagcttcagt taccggttca
     3181 cccaggtaat cttcagcggt tttcttcatt tttttcagca cttcagcaga aatctgcggc
     3241 ggtgccattt tctggccttt aacttcgacc catgcgtcgc cgttatcagc agcaataatt
     3301 ttgaacggca tgatggaaac atcacgctgt acttcttcgt cctggaagcg gcggccaatc
     3361 aggcgtttaa tcgcaaacag ggtgttttgc gggttcgtca ctgcctgacg tttagccggc
     3421 tgaccaacca gagtttcacc atcctgggta taggcaatga tagaaggcgt ggtgcgatcg
     3481 ccttcggcgt tctccagcac acgaggagtg gtgccatcca taatcgctac acaagagttg
     3541 gtagtaccca ggtcgatacc aattatttta cccatctaaa cgtctccact atatattcgg
     3601 tcatcatgtg gttgtgagtc tgtaataggg gcgaaacgtc tggtttcaac tgcccaattt
     3661 catttttttg cagactcact cactgcggtt gactactaaa tggggtcgta acccacgtca
     3721 tcaaggggga gatgcaaaaa ttttttgtgc ttttgcgcag aaatttcgcg atatggcagg
     3781 acaagacgta aaattgacag caattttatt catataaaga atgaatcgca gtcaaggtat
     3841 tcatcaaaaa attgtaataa aaagaaaaga ttacgtgcct gaatcttctc tttatcagca
     3901 gtaaactagt gggtattcat ccccctacct cttcccacta agagaatcct tatgaaatcc
     3961 gtttttacga tttccgccag cctggcgatt agcctgatgc tgtgctgcac ggcgcaggca
     4021 aacgaccata aactcctcgg cgtcattgca atgccgcgta acgaaaccaa cgctctggcg
     4081 ctgaaacttc ctgtttgtcg cattgtgaaa cgcatacaac tctctgccga ccatggcgat
     4141 ttacagttaa gcggtgcatc ggtttatttc aaagccgccc gtagcgccag tcagagcctg
     4201 aatattcctt cagaaataaa agaagggcaa accactgact ggatcaacat taacagcgat
     4261 aacgacaata aacgctgcgt ctcaaaaatc accttttccg gtcatacggt gaactcatcg
     4321 gatatggcca cgctgaaaat tatcggcgac gactaacgcc agaatataga agccacaaaa
     4381 aatgaatgtt aattacctga atgattcaga tctggatttt ctccagcatt gtagtgagga
     4441 acagttagca aatttcgccc ggctgctcac ccataatgaa aaaggcaaaa ctcgcctctc
     4501 cagcgtactg atgcgcaacg aactgtttaa atcgatggaa ggacatcccg agcaacatcg
     4561 ccgcaactgg cagctgattg ctggagaatt acagcatttt ggtggcgata gtatcgccaa
     4621 caaactgcgc ggacacggta aattgtatcg ggccattttg ctcgatgttt caaaacgatt
     4681 gaagctgaaa gccgacaaag agatgtctac gtttgaaatt gagcaacagt tactggaaca
     4741 atttctgcgt aatacctgga agaaaatgga cgaggaacat aagcaggagt ttctgcacgc
     4801 ggtcgatgcc agggtgaatg agctggaaga gctgctgccg ctgctgatga aagacaaatt
     4861 attggcaaaa ggcgtgtcgc atctgctttc cagccaactg acccgcattt tacgcaccca
     4921 cgcagcaatg agcgtacttg ggcatggttt gctgcgcggc gcggggctgg gaggccctgt
     4981 aggcgcggca ctaaatgggg ttaaagcggt cagcggcagc tcctatcgcg tgacgattcc
     5041 agccgtactg caaatcgcct gcctgcgccg gatggttagc gccactcagg tctgataccg
     5101 gaaaccgggg aaaattttca ttttcccctc aaaagatcgt agacactgcc ccactggctg
     5161 attattatgc cgcgccctga aaacactaca gttattcagg gaaattattt caccattcat
     5221 tcgatgatga tttttgagga attatgggca acactaagtt ggctaatccg gcaccgctgg
     5281 gcctgatggg cttcggcatg accaccattc tgcttaacct gcacaacgta ggttatttcg
     5341 ctctggacgg tattattctt gccatgggca ttttctacgg cggcatcgcg caaatttttg
     5401 ctggtctgct ggagtacaaa aaaggcaaca ctttcggttt aaccgcattc acctcttacg
     5461 gttctttctg gctgacgctg gttgcgattc tgctgatgcc gaaactgggt ctgaccgatg
     5521 cgccaaatgc acagttcctt ggtgtctacc tgggtctgtg gggcgtattt acgctgttta
     5581 tgttcttcgg cacgctgaaa ggcgcacgcg ttctgcaatt cgttttcttt agcctgaccg
     5641 tgctgtttgc cctgctggcg atcggtaaca ttgccggtaa cgccgcaatc atccactttg
     5701 ccggctggat tggtctgatc tgcggtgcca gcgcaatcta tctggcgatg ggtgaagtac
     5761 tgaacgagca gtttggtcgc accgttctgc cgattggtga atcccactaa ttgtcttgag
     5821 atccccccgc tcggggggat ttttttattc gctaacgtcg cgtcttgcac tcttcggtct
     5881 gaatgcagca accacttctg gtgccgtttc aacgtatggc ccttccagca actgaatgca
     5941 gtacggtacg ctggcaaaaa taccgtgtac cacaacgtta ccctcagcgt ccttcacacc
     6001 ttccagcgtc tctttaatag acttcggctg accgggtaag ttaaggatca gcgcctgttt
     6061 gcgaatcaca cccacctgac gcgaaaggat cgcagttggt acaaaatgca ggctgatctg
     6121 gcgcatctgt tcaccaaagc caggcatctc gcggtccgct actgccagcg tcgcatcggg
     6181 cgttacgtca cgacgcgccg ggccagttcc gcccgtggtg agcaccagat ggcaactcat
     6241 ttcatccacc agctcacaca acgtttgctc gatgatcgcc tgctcatcgg ggattaagcg
     6301 ggtttccagt tcaaacggcg tggttagcgc cgatgtcagc cattcttcca gcgcagggat
     6361 acctttatcc tgataaacgc cgctggatgc gcgatcagag atggaaacta agccaatacg
     6421 taaagtattc atcaagattt ttccgtttaa acagtctcgt taaacagaat gatacactgc
     6481 gaagggagtg acagacaggc ttcagaagag gtagcgtgac cgacttcccg gtcacgctaa
     6541 gaatgattac agcagatcgc cgatcatttt ttccagtttt tcctggtcaa tagcaaactt
     6601 acggatacct tccgccagtt tatctactgc cattggatcc tggttgtgct gccacaggaa
     6661 ctcggactca gtgatacgcg ccggacgcgc tttcacttcg ccggtgtaag acagtttacg
     6721 ttcgatagcc ccttcgctct cagccagctc tttcagcagt gccggtgcga tggtcagacg
     6781 gtcgcagcct gccagttcca gaatttcgcc gatgttacgg aagcttgcgc ccataaccac
     6841 ggtttcataa ccgtgctctt tgtagtactg gtagatttca gatacagaaa ccacgcccgg
     6901 atcttctgcc ggagcgtact ctttcttatc ggtattcgct ttgtaccagt caagaatacg
     6961 gccaacaaac ggcgagatca ggaacacgcc cgcttccgca caagcacgag cctgagcgaa
     7021 ggagaacagc agggtcaggt tacagttgat accttctttt tccagctgtt ccgcagcacg
     7081 gataccctgc caggtagaag ccagtttgat cagaatacga tcgttgctaa tacctgcatc
     7141 gttgtagagt ttgatcaggc gttttgcttt cgcaattgac gcttctgtgt cataggaaag
     7201 acgcgcatca acttcagttg agatacggcc cggaaccagt ttcaggattt ccagaccaat
     7261 atttactgcc agtttgtcag tcgcgtccac gatctgctgc gcgcgatcgt tgctctgctg
     7321 tttcgcccag gcgacagcat catcaatcaa cttacggtat tccggaatct gcgctgcgtt
     7381 aagaatgaga gaagggttgg ttgtggcatc ctgcggttga tacagcttca ttgccgcgat
     7441 gtccccagtg tcggccacta cggtggtgta ctgacgaagg gaggtcaatt tgtccgtcat
     7501 gatagtattt ctctttaaac agcttgttag ggggatgtaa ccggtctgcc ctgatgatat
     7561 cacgacgcaa cgtcagcgca accgccgaca agacggttat cgccaggcga gactgtttcg
     7621 gatttctgac actgcgttga tatcgcccgc catttttaaa caaaacctca tgtatgctac
     7681 gcagaagtta tcaagtacct cgtagcgtat atacttctta aacaattggt aacgtttaca
     7741 caggaaagtc atcgcgaccg gcaataagag ggatatgcat gccagatttt ttctccttta
     7801 ttaacagcgt cctttgggga tcggtaatga tttacctgct cttcggcgca ggttgttggt
     7861 tcacttttcg caccggattt gtgcagtttc gctacatccg ccagtttggc aaaagtctta
     7921 aaaatagcat tcatccacag ccaggcggtt taacctcatt tcagtcattg tgtaccagtc
     7981 ttgcggcgcg cgtgggtagc ggcaacctgg ccggcgttgc gctggctatt accgccggtg
     8041 gacctggagc cgtcttttgg atgtgggttg ccgcgtttat cggcatggcg acctcgtttg
     8101 ccgaatgttc ccttgcacaa ctttataaag aacgtgacgt caatgggcag tttcgtggcg
     8161 gaccggcatg gtatatggcg cgcgggctgg ggatgcgctg gatgggcgtt ctgttcgccg
     8221 tctttttgct catcgcctac ggcataattt tcagcggagt tcaggcaaac gctgttgccc
     8281 gagctctgag tttttctttt gattttcccc cgctggtgac aggcattatt cttgctgtct
     8341 ttgctctgct ggcaatcatt cgcggtcttc atggcgtcgc ccggctcatg caggggtttg
     8401 tcccgttgat ggcgataatc tgggtactga ccagcctggt gatttgcgta ataaatatcg
     8461 ggcaacttcc ccacgtcatt tggtctattt ttgagagtgc ttttggctgg caggaagcgg
     8521 caggcggcgc ggcgggatat accttaagcc aggcgattac taacggtttt cagcgcagta
     8581 tgttttccaa tgaggcggga atggggtcga cgccaaacgc ggcagcggca gcggcgtcct
     8641 ggcctccgca tccggcagcg caaggaattg tccagatgat tggcattttt atcgacaccc
     8701 tggtcatctg tacggcaagc gccatgctga tattactggc gggtaacggc acaacctaca
     8761 tgccgctgga aggtattcag cttatccaga aggcgatgcg ggtgctaatg ggttcctggg
     8821 gtgctgaatt tgttaccctc gtggttattc tgtttgcctt cagctccatc gttgccaact
     8881 acatttatgc cgaaaacaat ctcttctttt tacgcctgaa caaccctaaa gcgatctggt
     8941 gtttgcggat ctgcaccttc gcaacggtca tcggcggcac cttgctaagt cttccgctga
     9001 tgtggcaact ggcagatatc ataatggcct gcatggctat taccaatttg actgccatat
     9061 tactgctctc gcctgtggtt cataccattg ccagtgatta tctacgccag cgtaaactcg
     9121 gcgtgcgccc ggtgtttgat ccgttgcgtt atccggatat cggtcgccag ctttctcggg
     9181 acgcgtggga tgatgtttcg caggagtaat cacaactatc gatcaactca ttctcttttt
     9241 ttgctaaagt cggcataaat ttcctgcaag gactggatat gctgattctt atttcacctg
     9301 cgaaaacgct tgattaccaa agcccgttga ccaccacgcg atatacactg ccggagctgt
     9361 tagacaattc ccagcagttg atccatgaag cgcggaaact gacgcctccg cagattagca
     9421 cgctgatgcg tatcagcgac aaactggcag gtatcaacgc cgctcgcttt catgactggc
     9481 agccagattt cacgccggag aatgcccgcc aggcgattct ggcgtttaaa ggtgatgtct
     9541 acaccggctt gcaggccgaa accttcagcg aagacgattt cgattttgcc caacagcatt
     9601 tgcgaatgct ttccggcttg tatggcgtac tccgcccgct cgatttaatg cagccttatc
     9661 gtctggaaat ggggatccgt cttgagaatg cccgagggaa agatctgtat caattctggg
     9721 gagatatcat caccaacaag ctgaacgagg cgctcgcagc acaaggcgat aatgtggtga
     9781 ttaatctggc gtcagatgag tattttaaat cggtgaagcc gaagaaattg aatgccgaga
     9841 ttatcaagcc ggtgttcctc gatgagaaga acggcaaatt taagataatc agcttctacg
     9901 ctaagaaagc acgcggtctg atgagtcgtt tcattattga aaatcggctg accaaaccag
     9961 agcaactgac tggttttaat agcgaaggtt acttctttga tgaagcttcc tccagcaatg
    10021 gcgaactggt gtttaaacgc tacgagcagc gttaagtttg tagtcaatgc cggatgcggc
    10081 gtgaacgcct tatccggcct acaaaagcat gcagattcaa tatattgcag aattcacgta
    10141 ggcctgataa gacgcgccag cgtcgcatca ggcgctgaat gccggattgt tacccggcat
    10201 ttgtcattta gcggtgatgt ttgcctggac catgaccgcc gtgatgatca tgatgagctt
    10261 tcttatggtg acgcggcggt ggcggcggtc cgtgtgggtg ccagcgattg cctcgccatt
    10321 cataatgttg tttccaccag ccgtggtcgc gccagtgacc gccatcccag taataaccgc
    10381 gattatcacg atcgcctatc tgtaatttta ctgacgggac taacgtaatt tccgcagcct
    10441 gcgttgccat gggagcgacc agaaccaggg aaagtgcgag tacgatagat tgcatctttt
    10501 tcacaggtta ctccttttat cattgcccgt tatgggtcga tatccggaga ttacgcgatg
    10561 agagcggcgg ttgttattct ccgcaatcct aatctctaag ttaccgcatt tattgagaat
    10621 ttctcctttt tccctgtcgt ttctctccat attttcttca caaaaaagcc gggctgcata
    10681 aaagcaaacc cggcctgatt gagataatga atagatgtta ctgatgattc atcatcaatt
    10741 tacgcaacgc agcaaaatcg gcgggcagat tatgcgaaag caagggtaaa tcggcacgtt
    10801 ctgccagctc ttttggcaga tccaacgttt caccgagaat cgcttccacg ctctctttaa
    10861 atttcgccgg atgcgcggtg ccgaggaaca agccatattc gcctggattc aactggtcac
    10921 gcagcgcacg atacgctacg gcagcgtgcg gctccgaagt gtagcccagt tcttttaact
    10981 cacgcattgt ctgttgcgtg gtttcatcat ccacggcggc ataacccagc tctttcagtt
    11041 gccagatttt gcggcggaac aactcttcca cacgcggcca gttgttcggc tgactcacgt
    11101 ccatcgcgtt ggataacgtc gcctgagtcg ctttgggtga ccactgaccg tcatgcagga
    11161 aacgtggcac ggtatcgttc acgttggtcg cagcaataaa acgtttcacc ggcagaccga
    11221 gtgacttcgc cagcagaccc gccgtcaaat cgccgaagtt tccgcttggc accgagacaa
    11281 ccagctggtt gcgcgcttcc tgcggcagct gcgcaactgc ttcaaagtag tagcaaatct
    11341 gcgccagcaa ccggctaatg ttaatcgagt tagctgagtt taaccccagc gccactttca
    11401 gctcttcatc atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga
    11461 tggcaacagt ttcgatattg ccgcccaatg tacagaacag tttttcttgc agtggactga
    11521 ttttgcctcg tggataaagg ataaccactt tcacattcgg taaaccgtag aaagcatgag
    11581 ccactgccgc tccggtatca ccggaggtcg cggtcagaat ggtcactggc ttatcgcccg
    11641 caatatgggt cagcatttgt gccataaagc gaccgccgaa atctttaaat gccagcgttg
    11701 gcccgtggaa caattccaga caaccgacat cgctttcaac attggcgacc ggagccggga
    11761 aggcaaacgc cgcgcgcacg cgctcttcca ggatttcctg cgggatttca tcaccaataa
    11821 acgccgagag gatcttcgca ctgcgggtga caaaatccag cttcagcatc tcatcaattt
    11881 cagtcaggct gaattccggc aggtcgtgcg gaaaaaacag cccctgattt ttgcccaacc
    11941 cctgggttac ggcttgcgca aagctgacct gctcattgtg atctttaaga ttgtagagtt
    12001 tcatttagtt ttccagtact cgtgcgcccg ccgtatccag ccggcaaata tgaacaaaac
    12061 cttcctgatt ttgcaggtag ttcttaccca accagtcggc aacgcgctgg gcggtatccg
    12121 gcttgtcaca cagagcaaac aaggtcgggc cggagccgga gataccgctc gctaccgcgc
    12181 cgatttccgc gaccgcctgc cgcgcctgcc ggaagccagg cagtaaccgt tcacggtagg
    12241 gttctgcgat aacatctttc atcagcttcg cggcaagctc aggctgacgg gaatagcagg
    12301 cgtgaatgaa gcctgccaag tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg
    12361 ccggtaaaat agcccgggct tctgccgtcg agactttaat ccccggatac gccagcaccc
    12421 acagccactc atcaaaccct ggcacttgct ggctgatgat gtcgttttct tcgatcatca
    12481 actgcatacc accaagaaaa cacggtgcca cgttgtcgta atgaatgctg ccggagattc
    12541 gtccttccag ctcgcccatc aaagccagca aacgggtgtc attaagcggc ttgccgcagt
    12601 gttcattcat cgccatcaga gccgcgacca ccgaacaggc gctggagcct aagcccgaac
    12661 cgatcggcat attcttttcc agagtcatcg ccactggaat ttgcttgccc agctcctggc
    12721 aaaaacgctc ccagcactga taaacgatat tttcccgtgg ttctgacggc agcttatcgg
    12781 caaagcgtcc gaggttgttg agactgaatg tctctgccga ctcaaccgtg actacatctc
    12841 cgagcaatgc accatcaacg ggtgtcaccg ccgccccgag cacatcaaac ccgacgctca
    12901 tattggcact ggaagccggg gcataaactt taaccatgtc agactcctaa cttccatgag
    12961 agggtacgta gcagatcggc aaagacaccg gcagcggtaa cgtcattgcc cgcaccatat
    13021 ccgcgcagca ccaacggcag cggctgataa tagtggctat aaaaggccag ggcgttttcg
    13081 ccatttttca ctttgaacag cggatcatta ccatccactt cggcaatctt cacgcggcag
    13141 acgccatctt catcaatatt gccaacatag cgcaaaactt ttccttcatc acgggccttc
    13201 gccacgcgcg cggcaaagag atcgtcgagc tgtgacagat tcgccataaa agcggcaaca
    13261 tcaccctcag cgttaaactc tgcgggcagc acaggttcaa tttcaatatc cgccagctcc
    13321 agttcacgtc ccgtttcacg agcgagaatt aatagtttac gcgctacatc cataccagaa
    13381 agatcatctc gcggatccgg ttcggtataa cccatttccc gcgccagcgt agtcgcctcg
    13441 gagaaactca tgccttcgtc taacttgccg aagatataag aaagcgaacc tgaaagaatg
    13501 ccggagaact tcatcaattc atcaccagca ttgagcagat tttgcaggtt ctcaataacc
    13561 ggtaatccag ccccaacgtt ggtgtcatag aggaatttac gccgcgattt ttcagccgca
    13621 tgacgcaaca gatggtagta atccatcgac gaggtgttgg cctttttgtt cggcgtgaca
    13681 acgtggaaac cttcgcgcag gaagtcggca tattgatccg ccactgcctg gctggaggtg
    13741 cagtcaacaa tgaccgggtt cagcagatga tattctttca cgaggcgaat taagcgcccg
    13801 agattaaacg gctctttggc ttgcgccagt tcttcctgcc agttttccag atttaggcca
    13861 tgcacattgg tgagcagagc cttcgagtta gcaacaccgc agacacgtaa gtcgatatgt
    13921 ttattcttca accagctttg ctgacgcttc agttgctcca gcagcgcacc gccaacgcca
    13981 ccgacgccaa tcacaaacac ttcgataacc tgatcggtat tgaacagcat ctgatgagta
    14041 acgcgcacgc cagtggtcgc atcatcgtta tttaccacga cagagattga gcgttcagaa
    14101 gatccctgag caatagcgac aatgttgata ttggcgcggg ccagcgcggc aaagaatttc
    14161 gccgagatcc cacgcaaggt gcgcatacca tcacctacca ccgagataat ggccagccgt
    14221 tccgtcaccg ccagcggctc cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc
    14281 attgcccgtt cagctcgcac acagtcgctt tgcggaacgc agaaactgat actgtattcg
    14341 gaagatgatt gcgtaatcag caccacggaa atacgggcgc gtgacattgc agcaaagacg
    14401 cgcgccgcca tgccgaccat tcctttcatc cccgggccgg aaacgctgaa cattgccata
    14461 ttattcagat tggaaatgcc cttgaccggt aattcgtctt catcacgact ggcaccaatg
    14521 agcgtacctg gagcttgagg atttccggta tttttaatca ggcaagggat ctggaactgg
    14581 gcgatggggg taatggtgcg ggggtgaaga actttagcgc cgaagtagga aagctccatc
    14641 gcttcctggt aggacatcga tttcaacaac ctcgcatcgg gcacctgacg cgggtcgcag
    14701 gtatataccc cgtcaacgtc cgtccaaatc tcgcaacaat cggcgcgtaa acaggcagcc
    14761 agcaccgccg cggagtagtc ggaaccgttg cgtccaagta ccaccagttc gcctttttca
    14821 ttaccggcgg tgaaacctgc catcagcacc atgtgatcag ccggaatacg acttgccgca
    14881 atacggcggg tggactctgc aatatcgaca gtagattcga ggtaatgccc cactgccagc
    14941 agtttttcga ccggatcgat aacggtaacg ttgtgaccgc gcgcttctaa tacgccggcc
    15001 ataatggcga tcgacatttt ctcgccacgg caaatcagcg cagcgttaat gctatccggg
    15061 cactgcccta acaaactaat gccatgcagg acatgtttta tttgggcaaa ttcctggtcg
    15121 acgaaagttt tcaattgcgc cagcgggaat cccggctggg cggcggcgag tcccgtcaga
    15181 agttcggcaa aaatacgttc ggcatcgctg atattgggta aagcatcctg gccgctaatg
    15241 gttttttcaa tcatcgccac caggtggttg gtgattttgg cgggggcaga gaggacggtg
    15301 gccacctgcc cctgcctggc attgctttcc agaatatcgg caacccgcag aaaacgttct
    15361 gcatttgcca ctgatgtacc gccgaacttc aacactcgca tggttgttac ctcgttacct
    15421 ttggtcgaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tcctgtacgc
    15481 gtcagcccgc accgttacct gtggtaatgg taatggtgat ggtggtggtg atggtggtgg
    15541 taatggtggt gctaatgcgt ttcatggatg ttgtgtactc tgtaattttt atctgtctgt
    15601 gcgctatgcc tatattggtt aaagtattta gtgacctaag tcaataaaat tttaatttac
    15661 tcacggcagg taaccagttc agaagctgct gtcagagact ctttttttaa tccacacaga
    15721 gacatattgc ccgttgcagt cagaatgaaa agctgaaaaa tacttactaa ggcgtttttt
    15781 atttggtgat atttttttca atatcatgca gcaaacggtg caacattgcc gtgtctcgtt
    15841 gctctaaaag ccccaggcgt tgttgtaacc agtcgaccag ttttatgtca tctgccactg
    15901 ccagagtcgt cagcaatgcc atggctcgtt cgcgtaaagc ttgcagttga tgttggtctg
    15961 ccgttgtgtc acttttcgtc ggttgttgta ttaatattgc taattgatag caatagacca
    16021 tcaccgcctg ccccagattg agcgaaggat aatccgccac catcggcaca ccagtaagaa
    16081 cgtcagccaa cgctaactct tcgttagtca gcccggaatc ttcgcgacca aacaccagcg
    16141 cggcatggct catccatgaa gatttttcct ctaacagcgg caccagttct actggcgtag
    16201 cgtagtaatg atatttcgcc cgactgcgcg cagtggtggc gacagtgaaa tcgacatcgt
    16261 gtaacgattc agccagtgtc gggaaaactt taatattatc aataatatca ccagatccat
    16321 gtgcgaccca gcgggtggct ggctccaggt gtgcctgact atcgacaatc cgcagttcgc
    16381 taaaccccat cgttttcatt gcccgcgccg ctgccccaat attttctgct ctggcgggtg
    16441 cgaccagaat aatcgttata cgcatattgc cactcttctt gaccaaatga ccgcgaacag
    16501 ggtgatcact gtcaacttat tacgcggtgc gaatttacaa attcttaacg taagtcgcag
    16561 aaaaagccct ttacttagct taaaaaaggc taaactattt cctgactgta ctaacggttt
    16621 aattgttaaa aaatgctaca tatccttctg tttacttagg ataattttat aaaaaataaa
    16681 tctcgacaat tggattcacc acgtttatta gttgtatgat gcaactagtt ggattattaa
    16741 aataatgtga cgaaagctag catttagata cgatgatttc atcaaactgt taacgtgcta
    16801 caattgaact tgatatatgt caacgaagcg tagttttatt gggtgtccgg cccctcttag
    16861 cctgttatgt tgctgttaaa atggttagga tgacagccgt ttttgacact gtcgggtcct
    16921 gagggaaagt acccacgacc aagctaatga tgttgttgac gttgatggaa agtgcatcaa
    16981 gaacgcaatt acgtacttta gtcatgttac gccgatcatg ttaatttgca gcatgcatca
    17041 ggcaggtcag ggacttttgt acttcctgtt tcgatttagt tggcaattta ggtagcaaac
    17101 atgcagaccc cgcacattct tatcgttgaa gacgagttgg taacacgcaa cacgttgaaa
    17161 agtattttcg aagcggaagg ctatgatgtt ttcgaagcga cagatggcgc ggaaatgcat
    17221 cagatcctct ctgaatatga catcaacctg gtgatcatgg atatcaatct gccgggtaag
    17281 aacggtcttc tgttagcgcg tgaactgcgc gagcaggcga atgttgcgtt gatgttcctg
    17341 actggccgtg acaacgaagt cgataaaatt ctcggcctcg aaatcggtgc agatgactac
    17401 atcaccaaac cgttcaaccc gcgtgaactg acgattcgtg cacgcaacct gctgtcccgt
    17461 accatgaatc tgggtactgt cagcgaagaa cgtcgtagcg ttgaaagcta caagttcaat
    17521 ggttgggaac tggacatcaa cagccgttct ttgatcggcc ctgatggcga gcagtacaag
    17581 ctgccgcgca gtgagttccg cgccatgctt cacttctgtg aaaacccagg caaaattcag
    17641 tcccgtgctg aactgctgaa gaaaatgacc ggccgtgagc tgaaaccgca cgaccgtact
    17701 gtagacgtga cgatccgccg tattcgtaaa catttcgaat ctacgccgga tacgccggaa
    17761 atcatcgcca ccattcacgg tgaaggttat cgcttctgcg gtgatctgga agattaatcg
    17821 gctttaccac cgtcaaaaaa aacggcgctt tttagcgccg tttttatttt tcaaccttat
    17881 ttccagatac gtaattcatc gtccgttgta acttctttac tggctttcat tttcggcagt
    17941 gaaaacgcat accagtcgat attacgagtc acaaacatca tgccggccag cgccaccacc
    18001 agcacactgg ttcccaacaa cagcgcgcta tcggcagagt tgaggagtcc ccacatcaca
    18061 ccatccagca acaacagcgc gagggtaaac aacatgctgt tgcgccaacc tttcaatacc
    18121 gcctgcaaat aaataccgtt cattaacgcc ccaatcagac tggcgattat ccatgccacg
    18181 gtaaaaccgg tatgttcaga aagcgccagc aagagcaaat aaaacatcac caatgaaagc
    18241 cccaccagca aatattgcat tgggtgtaaa cgttgcgcgg tgagcgtttc aaaaacaaag
    18301 aacgccataa aagtcagcgc aatcagcaga atggcatact tagtcgcccg gtcagttaat
    18361 tggtattgat cggcgggcgt cgtcactgcg acgctaaacg cggggaagtt ttcccagccg
    18421 gtatcattgc ctgaagcaaa acgctcaccg agattattag caaaccagct gctttgccat
    18481 tgcgcctgaa aacctgactc actaacttcc cgtttggctg gtagaaaatc acctaaaaaa
    18541 ctgggatgcg gccagttgct ggttaaggtc atttcgctat tacgccccgc aggcaccaca
    18601 gaaagatcgc cggtaccgct taaattcagt gccatattca gcttcaggtt ctgcttccgc
    18661 cagtcccctt cgggtaaagg gatatgcacg ccctgcccgc cttgctctaa cccgatgccg
    18721 ggttcaatag tcagcgccgt tccgttaact tcaggcgctt tcaccacacc aataccacgc
    18781 gcatccccga cgctaatcac aataaatggc ttgccaaagg tgatatttgg cgcgtcgagt
    18841 tcgctaagac gcgaaacatc gaaatcggct tttagcgtta aatcactgtg ccagacctga
    18901 ccggtataaa tccctatctt gcgttcttcc acgttctgat tgccatcaac catcaatgac
    18961 tcaggtaacc aaaaatggat aaaacttcgt ttccgctcca cggttttatc ctcttcctgc
    19021 accgtataaa gctcggtcac aggaatagcg ataagaggcc caacgagttt ttgcggcccg
    19081 ctggtacttt ggcgaatcgc atcttccaca tcgctacggt aatcggcacg ttcgacaatc
    19141 acctgccgaa tcagcattat cggaatcaac aacagcaata ctgcaccaaa cagggtggtc
    19201 attttccaga acagggggga tttcaacata gtcttctcct ttgcaatggc agcaggatac
    19261 gaaggctatg tgggaagaat ttgaagctat gtgaagtgac ggtgaagccg aagcgaggcc
    19321 agcacgccac cttcctgcac gttgcgcagc gtgacttcgc cgttaaacaa acgggcgacc
    19381 tcactgacaa acgccaaccc cagaccgctg cttttttgcc catttgcacg cggcaaagag
    19441 taaaagcgtt caaaaatacg tgacagcgcg tagtcaggaa taccactacc ggtatccagc
    19501 accttaaggg cgacgtgttc ctgatccact tcggcgctta gcgttatgcg accgctcttg
    19561 ggggtaaaat cgatggcgtt atccagtaaa ttccccagcg cctgctccag taacgccggt
    19621 tcagcagcaa cgttaacctc agtaggcata acatgcaaag tgatgttttt ttctgccaac
    19681 tgcacggtgc gcgcttcgct gacgcggcga aataatgccg ccacatcaac agcagtcaga
    19741 acgacttcct gacgattctc cagtcttgcc tggcgtagta acgtttctac caatgcctgc
    19801 atacgcgcat tttgcgtcag aatgttgtcg gtaaaacgag ccaccacttc cggtggcgga
    19861 ccttcgcgta aaatttccgc cgcgccacga atcgccgcca gtgggctttt tagctcatgg
    19921 gttaacgcat aaacatactg ctcaatatag tttttccctt ccagcttcac gcgcatactt
    19981 tccagcgcct gcgcaagctt acgcaactcg ctactgccga gttcaggaag aggaacgggc
    20041 ttattgtcag tgacggaatc ggcatagcga gtgagccggg caatggagcg gttgatccac
    20101 caaaccatgc ctgcgccaat caccagtgca atccccagca aaatggcgct ggcccataaa
    20161 attcgccgct cgctgcgctt aatgaccgga gccatcgccg cgttcggttt gcctacgctc
    20221 aaaacgccaa taagccgcga gccgtccata atcggcgcgg caacatacat caccgaactt
    20281 tcgggatcgg caggattttg caacgtgctg cgcgcaccat actgaccacg caacgttagc
    20341 cagacgtcat tccagcgcga ataatcctgc ccaacggctt tatttgccga atcgaacaat
    20401 actttgccct gcgcatcggt catatagaca tgatattcat tgcgcacttt gttaatgcca
    20461 ccgatattag cgcgaaacgg gcgatgttgt agctgattaa acgcctgcgc cagttgccca
    20521 tgcgttgggt ccccagagag caaatcggga cgcgccagct ccgccagcaa cgttgcggtg
    20581 tcgatcagcg tcccctccgt tgctcttcgc acgcccggtt taacttcttt gacaaaaata
    20641 gccagtacga accaggccgc caccgccacc agtaaaaaat agcccagcaa caaccgcatg
    20701 ccgatacgca ttacaggctc ctcaggctgt atcccatgcc gcgatgagta ttaatcggtg
    20761 aaagatcggg gttgatggcg cgcagcttgg cacgcagcgt tttaatgtgg gtatcgacgg
    20821 tgcgatcgta ggtgtcctgc gcatcttccc acacgatatc catcagttgc tggcgggacc
    20881 agacgcggcc tggcgacttg agtaacgtct tcagcaataa aaactcatac cgcgtcagcg
    20941 ttaatggcgt gtcaaaccag ctgatctgcg ccgcgggttc attcaattca aaatgaccaa
    21001 tacggatgac gggagacggc gtcgagaact tcttcacccg acgcagcaag gtgcgcaccc
    21061 tagcgcacac ttcgcggggt gaaaacggtt tagccacata gtcgtcagcg ccaatttcca
    21121 gcccaagcag gcgatcgacc tcttcacttc gggccgtcag gaacagtaca ggtaacgccg
    21181 gatggagcgc cagtaactgg cggcacaatt caaagccact gatatcaggc aaaccaacgt
    21241 cgagaatcat gacatcggga acctgctggc gagctttatc cagcaccggc aagcctcgct
    21301 caaagacctc gacggcaaaa ccttcctgct gcaacatgta gaccagcgtg tcggctatcc
    21361 cttgctcatc ttccactaac cagaccgttt cccgttgcat aaatcgcctc tgttattgtc
    21421 gccacggcat gacaggaacc gcgctaatcg cgtttttcgg cgaaccttct acaactttgt
    21481 cggagtaagc cagataagcg agcgcgttgc gtttggcgtc ataaaagcgc acgacctgta
    21541 acgacttaaa gaccagggac gtgcgttttt tgaatactac ctcgccctga gctttgccgt
    21601 ttttaatacg atccgacagt tcaatcggcc cgacttgctg acaagaaata gccgcatcgg
    21661 aggtatcttc cgccagaccc aatccccctt taataccacc tgttttcgcc cggctcacat
    21721 aacaggtgac atttttcaca tcgggatcgt caaaggcttc cacaacaatt ttgtgatccg
    21781 ggccgatcat tttaaatacg gtgtcgaccg aaccaatctc ttctgcatga gccaatggcc
    21841 ccagcattat caggcttaaa gacaggatca aatgcttgta tttcatattg ttaccatttt
    21901 tatctattac acgagcaatt agttcgtcac ggtaaataac atattgatta agaacaaaat
    21961 ctcaatactt ttatttccgt caagacctaa aacatactct actaagaaaa aaacactgaa
    22021 tgctaaaaca gcaaaaaatg ctattatcca attacctgat gtcaggtgct cgttgttgaa
    22081 aggatgagga tattttatgg atcaggccgg cattattcgc gaccttttaa tctggctgga
    22141 aggtcatctg gatcagcccc tgtcgctcga caatgtagcg gcgaaagcag gttattccaa
    22201 gtggcactta cagagaatgt ttaaagatgt cactggccat gctattggcg cgtatattcg
    22261 tgctcgtcgt ttgtcgaaat cggcggtcgc actacgcctg actgcgcgtc cgattctgga
    22321 catcgcgctg caataccgct tcgactctca acagacattt acccgcgcat tcaagaagca
    22381 gtttgcccag actcctgcac tttaccgccg ttctcctgaa tggagcgcct ttggtattcg
    22441 cccgccgctg cgtctgggtg aattcactat gccagagcat aaatttgtca ccctggaaga
    22501 tacgccgctg attggtgtta cccagagcta ctcctgttcg ctggagcaaa tctctgattt
    22561 ccgccatgaa atgcgttatc agttctggca cgattttctc ggcaacgcgc cgaccattcc
    22621 gccagtgctc tacggcctga atgaaacgcg tccgagtcag gataaagacg acgaacaaga
    22681 ggtattctat accaccgcgt tagcccagga tcaggcagat ggctatgtac tgacggggca
    22741 tccggtgatg ctgcagggcg gcgaatatgt gatgtttacc tatgaaggtc tgggaaccgg
    22801 cgtgcaggag tttatcctga cggtatacgg aacgtgcatg ccaatgctca acctgacgcg
    22861 ccgtaaaggt caggatattg agcgatacta cccggcagaa gacgccaaag cgggagatcg
    22921 cccaattaat ctgcgctgtg aactgctgat tcctatccgc cgttaacgct gcagctcatc
    22981 taatgcaggg gcgtccagat gcgagatgtc ccctgcggtt tcaacgaccc agccagacgc
    23041 cagccacagg ctttcctgat aatccacgcg cgaaatagaa cagttacgca gacgtaagcg
    23101 gcgctctgcc catgctggta atccgagaat cgtactcacc agacatccca gtgcaatacc
    23161 atggctcacc agcagcggtc ggcttccctg cggtaagtcc cggcaagatt ccagtgccgc
    23221 attgacgcga tcgctgagct cctgcattga ctcgccttca ggaatacgcc catcaacggt
    23281 gccattgacc agctgccgac gccagttctc ttcttcttcg gtcagagaat cgatatgtct
    23341 tttttccagc acacccatgt ttaattcacg caggcgagaa tcaaagatga tgtcacagcc
    23401 gcaggcctgg gcgatgattt ccgccgtacg ccgggtgcgt cctaaatcgc tactgatgat
    23461 atgcgtaatg ccaagctctt tggcacgcgt cgccacctgc atcgcctgtt gctcaccttt
    23521 ggcggtcagc gggctgtcag actggccctg aatacgtcgc tcggcgttcc actgcgtttc
    23581 accgtggcgg actaggtata cctgtaacat gctttttttc cgtatactgc tgtaacgtta
    23641 attttctgag taatgctgat tatgcaccaa gttgtctgtg cgaccaccaa tcccgctaaa
    23701 attcaggcca ttctgcaggc atttcacgag atcttcggcg aaggatcctg ccatattgca
    23761 tccattgccg tcgagagcgg tgtaccggaa cagccctttg gcagtgagga aacgcgcgct
    23821 ggcgcacgaa accgggtagc caatgcccgc cgtttacttc cagaggctga tttttgggtg
    23881 gcgattgaag ctggcattga tggcgacagc actttcagtt gggtggtgat tgaaaacacc
    23941 acccagcgcg gcgaggcgcg ttcggcaact ttgccgttgc ctgcggtgat attggaaaaa
    24001 gtacgtgaag gtgaggcgct tggtcccgtg atgtcgcgtt ataccggtat tgatgagatt
    24061 ggtcgtaagg aaggggcgat cggcgtgttt accgccggga aactcactcg cgccagcgtc
    24121 tatcaccagg cggtgatcct ggcgctgagt ccgtttcata atgcggtgta ttaagcgcct
    24181 gatgcgacgc tgccgcgtct tatcatgcct accaaacata ttgaaattac gggtatttgt
    24241 aggacggata aggcgttcac gccgcatccg gcattcggtg cacgatgcct gatgcgccac
    24301 gtcttatcag gcctacaaaa tcaatcgctt ttcagcaaca cctcttccag ccactggcgc
    24361 agctccacgg gcgcggcttt caggctgtta gatccacgcg taatcgtcgc gatgcctgcg
    24421 ccgagttcat tttttaactc acgctggctc atttcgccgc gcaacagctc ttcgacaata
    24481 cgcacgcgag tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt taacaacggt
    24541 aaatggagat cgttttggta ggcattctta agcaggtcga caaaacgtaa ccactcctgg
    24601 tgacgctgtt ctgccatcgc tgctgaatag ggtgattgtt gggccataat atgtcgccat
    24661 tgttagcggg ggaagcaaaa tgcctccccc ggttgtactc gctaaagagt acgatagcat
    24721 atcataaacg tgcggatcag taacgacgtc cccattccgt ggcgctcatc aacgtcggtt
    24781 tatcccccat gaaatagcgg tagtaagcgt cataagccag cacgttcttc acataaccgc
    24841 gcgtctcgga gaacggaata ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt
    24901 tgccaagcca ggttcgcacc cgtccaggtc cggcgttata agctgctgag gagaaaatac
    24961 ggttattacc aaattgctga taaacatatt gcaggtaact ggtgccaatg ttgatattcg
    25021 tttccggatc cagcaattgc ccagggctgc tataaccagg aatagagaac atcttcaccg
    25081 tatgggtcgc cgtgcccggc ataatctgca tcaggccgct ggcccctacc ggtgatttca
    25141 ctttcggatt ccaggcgctc tcctgacggg caatcgccat cgcatagctt tgcgggatct
    25201 ccttcccact ggtgtaacgt ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca
    25261 gatgatccca aagcttcccg gcgatcgttg cctgaacgct aagatcccac cattggttgt
    25321 tgaaagcata ccgcgccagt tgagcctgct ctgtttttga cttgctcttc accagattgg
    25381 cccactcgct acgcgcggtg ttatcgagat tccagtacat caactcgcgc acgcgcgcca
    25441 tctccggccc ctgagtcagg gcgctgtcaa cattctgcgg cgctttatca atcttcagct
    25501 catactcttc gccgatgcgt tgtgcagcaa ccatcgggta gaaaccacgc tgttgcatga
    25561 gttgatgcaa aatctctttt gcttcagctt cacgtccgcg ttccagcaat aaatccgcct
    25621 gccagtaacg ccattcatct ttctctttcg cttccatagg cagacgcgcc agccaggtat
    25681 tcaggccgcg acgatcgcca gtgccaagcg ccattcgcac acggcgttca ataagcgaag
    25741 tagattgcga gcgcataatg gcgtcatcgc gccatttcgc ctgctcgtcg gtgacatcgt
    25801 tgcccatcaa acgccaggcg acgatatcgc gcagctcctg aatctgatct tcattaagct
    25861 gctgcgcctg ggcaagcgat gggatcatca gccgtgcatt ttcagcatcc tgccgcgcca
    25921 cactggcaaa cgccaccgcc gccatttgac gggtaaaatc ggtcgctccg gttgtacgcg
    25981 cgaaggtcag taccgtattg gggttgttcg ccagtgaaat gattgccgag gcgatagtct
    26041 ggtaatcggc aggcatctgc cctgccagca ctgttaccag gccagtgtta cccgctttca
    26101 tcgccagacg gatacgctct aaatacgcca gcggatcttg tttacctgac gcacgccaga
    26161 cgctaaataa cttgtcacag gcgttaggct ggctcttgcc ggttagccac agctctttcg
    26221 ccccttgcca ggcttcttca ctctgcccgg tgttccattt cgcatagtag taattacatt
    26281 gtgcttcggt agttccgggc ttttccgggc taaaggctaa caggccacgc cagtcttcac
    26341 gccgcgccag ttcattgacg aaacgagatt gcagcgtgcg agcgggagga agcgtggggt
    26401 tagcgcgaac aaagttagtg accgtcaccg ccggttgatt catcaggtca tcggtgatct
    26461 ggcggtattc caggtaggga taaagcggat aatccttcag tccaggcatc atttgttcga
    26521 ccacatccat ttgtcgatta tcccaggcct gcttgatttg cgcgtaacga ctacgctgct
    26581 catccagtga gtcggctcgc gccacgctgc tgaccgtcag cagacagaca ccggcagcca
    26641 acagccgcca ggtaacttgt ttggcttttt ccacaagcgc atcctctaag tgtaaataca
    26701 tcaatgcagc gtcatgccgc gtaatagatt tatgctaacc agtcattgcc gtttacgcca
    26761 cgttacggac acttttttac ttttactgcg agggtgatcg accggctggg attaggcggg
    26821 gaaaaaggct acacttcgcc tttgaaaact catcatcacg ataaacatag aggcgaagtc
    26881 caacgtggct caattcgttt ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg
    26941 tcatattttg aaaaacatct ctctgagttt cttccctggg gcaaaaattg gtgtcctggg
    27001 tctgaacggc gcgggtaagt ccaccctgct gcgcattatg gcgggcattg ataaagacat
    27061 cgaaggtgaa gcgcgtccgc agccagacat caagattggt tatctgccgc aggaaccgca
    27121 gctgaacccg gaacacaccg tacgtgagtc cattgaagaa gcggtttcag aagtggttaa
    27181 cgccctgaaa cgcctggatg aagtgtatgc gctgtacgcc gatccggatg ccgattttga
    27241 caagctggcc gctgaacaag gccgtctgga agagatcatt caggctcacg acggtcataa
    27301 tctgaacgta cagctggagc gtgcggcgga tgcgctacgt ctgccggact gggacgcgaa
    27361 aatcgctaac ctctccggtg gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga
    27421 aaaaccagac atgctgctgc tcgacgaacc gaccaaccac ctggatgccg aatccgtggc
    27481 ctggctggaa cgcttcctgc acgacttcga aggcaccgtg gtggcgatta cccacgaccg
    27541 ttacttcctc gataacgttg cgggctggat cctcgaactt gaccgcggtg aaggtattcc
    27601 gtgggaaggt aactactcct cctggctgga gcagaaagat cagcgtctgg cgcaggaagc
    27661 ttcacaagaa gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg
    27721 aactaaaggc cgccagtcga aaggtaaagc acgtctggca cgctttgaag aactgaacag
    27781 caccgaatat cagaaacgta acgaaaccaa cgaactgttt attccacctg gaccgcgtct
    27841 gggcgataaa gtgctggaag tcagcaacct gcgtaaatcc tatggcgatc gtctgctgat
    27901 tgatgacctg agcttctcga tcccgaaagg agcgatcgtc gggatcatcg gtccgaacgg
    27961 tgcgggtaaa tcgaccctgt tccgtatgat ctctggtcag gaacagccgg acagcggcac
    28021 catcactttg ggtgaaacgg tgaaactggc gtcggttgat cagttccgtg actcaatgga
    28081 taacagcaaa accgtttggg aagaagtttc cggcgggctg gatatcatga agatcggcaa
    28141 caccgagatg ccaagccgcg cctacgttgg ccgctttaac tttaaagggg ttgatcaggg
    28201 taaacgcgtt ggtgaactct ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct
    28261 gcaggttggc ggcaacatgc tgctgctcga cgaaccaacc aacgacctgg atatcgaaac
    28321 cctgcgcgcg ctggaaaacg ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca
    28381 cgaccgttgg ttcctcgacc gtatcgccac gcacattctg gattaccagg atgaaggtaa
    28441 agttgagttc ttcgaaggta actttaccga gtacgaagag tacaagaaac gcacgctggg
    28501 cgcagacgcg ctggagccga agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa
    28561 atgccggatg cggcgcgaac gccttatccg gcctacaaaa ccagcaattt caatacgttg
    28621 taaaactgta gacctgataa gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc
    28681 cggatgcggc gtgaacacct tttccggtct acaacagcct tagcatcctc aatttcgtgg
    28741 cgggccaccc gtgcaggttt tttcaaacct tgtttaatct cgacagcctc ttccagagag
    28801 gttttcaact catcaaaaaa actcatttta tacctcgcct ttgagcagtt tcatcacggt
    28861 tatctctgct cccccatcat ctcccgcacc agctccacgc agcgcaggaa acggctgtcg
    28921 taatcttcct cttcaacccg cacaaattcg atattgttct cttccagcat ctccaccagc
    28981 agattctgga actctttacg atccaccgaa ctgccgaggc tgcgtaaacc atccaccacc
    29041 cacggcgtgt tgttctccag caggatcacc agatcgaaac ggtattcatc aatcagcgcc
    29101 tgcacgaacg gatgttcccg cccttcgtac tttttgcaga acgcctgagt ggtgacaaaa
    29161 tcggtatcga taaatgccac tttattggca tatttcaccg caaaatcaat gtattgagcg
    29221 tggcccagcg cgattttatc gtagtcagaa tactgcaatg cgatctcatc accgccgagg
    29281 tgtgaaaaga cataatcgcg gccatattcc cacgcactgg tggtgttgaa gatattggca
    29341 agtttgttta ccagggtgga tttaccgctc gactcgccgc caaggatcgc cacggtacgc
    29401 acaaaaaacg gcttcacttc ggtaggaata tattcccagt agcggaacgg gttttcgcgg
    29461 atctgcgcac cgctgatact cataaaggta cgtttcggat cgaccagcac cgtctcgatc
    29521 cccagatgtt ccatatactg tggcgcatcg gcttcttccg aggtgtagat cagatccggc
    29581 tggatcccct tttcagccat aaactttttg ataccgttgc tccacacatc ccagccgtgc
    29641 ggatacggct ccatgccctc ttcgttgaaa gcatgaatgc gaatattttt ctgatattta
    29701 aaagtttgca ataaccagcg cagacgatcc ggcacggtcg gctgctgcga catggcactg
    29761 tcttcgaaca acgcgcggtc acgggtatcg tcaaaaccca taatgatatg cagctcgtca
    29821 acctggctac aggcgcgctg gataaggtag atatgtccgg tatgcagtgg gtagaactta
    29881 ccgaatacga caccgatcgt tttcttctgc cggggaaact caagccccaa aaaacggtgc
    29941 aacgcctcca gcttttgcgc gctggggctt ttgattttgg cattcagtaa ctggcttaaa
    30001 taccctttgg tcataccgct ggcatcagct acctgctgta gcgtgcagcc ctgttgcttg
    30061 atggcagttt tcaggtaatc aaatgacgac atatctccct ccgtatttct cattataagt
    30121 cgtcgaacac gctaagcgcg tcggagagtt ttttaacgcc aaaaatctgc atcccttccg
    30181 gcgctttttt cggtacgtta gccgccggaa caatcgcccg gcgaaaaccg tgtttcgccg
    30241 cttcagagat tcgttcctga ccgctgggca ccgggcggat ctcccctgcc agcccgactt
    30301 caccaaacac caccagatcc tgcggtagcg gtctgtcacg caggctggaa accatcgcca
    30361 gcagtaacgc taagtcggcg ctggtttcgg ttacctttac gccgccgacc acgttcacaa
    30421 acacatcctg atcggccatt tgcagaccac cgtgacggtg caagacagcc agcaggattg
    30481 ccagacggtt ttgttccagc cccactgcca cgcggcgcgg attcgccatc atcgagtgat
    30541 cgaccagcgc ctgaatctcc accagcaacg gacgcgttcc ttcccacacc accatcacgg
    30601 agctgccgga ggtcacttca tctccgcgac ttaagaaaat tgccgaaggg ttgctgactt
    30661 cacgcagccc ctgttcggtc atcgcgaaga cgcccagctc attcaccgcg ccgaagcggt
    30721 ttttatggct gcgcaaggtg cgaaaacggg agtcggcatc gccatctaaa agcaccgaac
    30781 agtcgataca gtgttccagc actttcgggc cagccagcga accatctttg gttacgtgcc
    30841 ccaccatgac aatcgccaca ccgcgcgttt tggcgaagcg tgtcaaataa gccgccgttt
    30901 cacgcacctg cgccacgcta ccaggcgatg actgtacatc cgccatatgc atcacctgga
    30961 tcgagtcaat taccatcagc ttcggttgct cttcttcggc aatcaggcag atctgttcga
    31021 tgctggtttc cgacaacata ttgagattgt cagtcggcag gccaaggcga tgagcgcgca
    31081 ttgccacctg ttgcagcgac tcttcgccgg tgacatacag cgttttcatc tgctgggcca
    31141 gtttgcacag cgtttgcagt agcagcgtgg atttccccgc accagggtta ccgccaatca
    31201 gaatggcact tcctggcacc acgccgccgc ctagtacgcg gtcgaactct ttaaatccgg
    31261 tggaaaaacg cggcagctct tcaaggctga tatcggagag tttctggact tttgccaccc
    31321 cggcgctacc ggcatagccg ctgagacgct cgttacgcgc caccgtaggc gacgcagcaa
    31381 gacgcacctc ggtgatggtg ttccaggcat gacaggcact gcactgcccc tgccagcgcg
    31441 gataatcggc cccgcattca ttacaaacaa aggcgcgttt tggagctttt gccacggtgt
    31501 acctcgttaa tgctgtgccg cccgcaggat ggcgggcgag caattacttc tgattcaggc
    31561 tgcctgagag gatgcagaat acccccatca ggtcagcgtg acggatggtg acttccgcct
    31621 tttcattcac ttttggcttg gcatggtagg caatccccag ccctgccgct ttgatcatcg
    31681 gcaggtcatt ggctccatcg ccaatcgcca cggtctgcgc cagcgggatt tcatactcct
    31741 gcgcgaggcg agtcagagtt ttcgctttgt actgcgcgtc tacgatgtcg ccgatcacat
    31801 tgccggtaaa tttaccgtcc atgatctcca gttcattggc taccacggct gtcaggcgca
    31861 gcttgtcgcg caggtattca gcaaagaaag taaagccgcc ggaggcaatc gccactttcc
    31921 agcccagcgt ttccagcttg agcaccagtt gcgttaagcc tggcatcagc ggcagatttt
    31981 cacgcacctg ttgcagaata ttggcgtcag cgcctttcag cgtcgccacg cggctgcgca
    32041 ggctggcggt aaaatcgagt tcgccgcgca tcgcccgttc ggttacttcc gccaccatct
    32101 cgcccgttcc ggccagtttg gcaatttcat caatacattc aatctggatg gcggtggaat
    32161 ccatgtccat caccagcaaa cccggcgtgc gcaggtgcgg gatttttccc agcggcgcga
    32221 catccagctg cgcttcgtgg gccaggcgtg tagcccgtgc ggtgagtgaa cctgccagac
    32281 gaatcacctg ataatcttcc acgcaccagg cggcaacaat caccatcgcc gcacccagtt
    32341 tgctctggta ttgggtcaga cgctgtttat ccagcccacg accatacagc agccagccgc
    32401 tacgacctgc gtggtaatcc agtggcatca cttcatcacc acttaatgaa agaggcagac
    32461 ccggccataa agagacatct tcaggcaggt cgcaccaggt aatgttaggc attaaggctc
    32521 ctgtaaaatc gttcgaagca gggaaaataa cgcatgaggc taccttgtat ccattgcttc
    32581 tggcaacatt aagtctcaaa ttttcaaagg gtggaagatg gctcgcacaa aactgaaatt
    32641 ccggctgcat cgggcagtga ttgtcctgtt ctgtcttgcc ttgttagtgg cactgatgca
    32701 gggggcgtca tggtttagtc aaaaccacca gcgacagcgt aatccacagc tggaagaact
    32761 ggcccgcacc ctggcgcgtc aggtgacgct gaacgttgca ccgctgatgc gtaccgactc
    32821 accggatgaa aaacgcattc aggcgatcct cgatcagtta acggatgaaa gccgtatcct
    32881 cgacgcgggt gtgtatgacg aacaaggcga tcttatcgca cgttctggcg aaagcgtcga
    32941 agtgcgcgat cggctggcgc tcgacggtaa aaaagcaggc ggctatttta accagcagat
    33001 tgtcgagcca attgccggga aaaacggacc gctcggctat ctgcgcctga cactcgacac
    33061 ccatacgctc gccaccgaag cccaacaggt ggataacacc actaacattt tacgcctgat
    33121 gttgctgctc tcactggcaa tcggcgtagt gctgacccgc acgctgctac agggtaaacg
    33181 cacccgctgg cagcaatcgc ccttcctgtt aaccgccagc aaaccggtgc cggaagagga
    33241 agaaagcgag aaaaaagagt gccccattac tacaagaaag gaaatcgtta tgtccacatt
    33301 acgcctgctc atctctgact cttacgaccc gtggtttaac ctggcggtgg aagagtgtat
    33361 ttttcgccaa atgcccgcca cgcagcgcgt tctgtttctc tggcgcaatg ccgacacggt
    33421 agtaattggt cgcgcgcaga acccgtggaa agagtgtaat acccggcgga tggaagaaga
    33481 taacgtccgc ctggcgcggc gcagtagcgg tggcggcgcg gtgttccacg atctcggcaa
    33541 tacctgcttt acctttatgg ctggcaagcc ggagtacgat aaaaccatct ccacgtcgat
    33601 tgtgctcaat gcactgaatg cgctcggcgt cagtgccgaa gcgtccggac gtaacgatct
    33661 ggtggtgaaa accgccgaag gcgaccgcaa agtctcagga tcggcctatc gcgaaaccaa
    33721 agatcgtggc ttccaccacg gcaccttgct gctcaatgcc gaccttagcc gcctggcaaa
    33781 ctatctcaat ccggataaaa agaaactggc ggcgaaaggc attacttcag tgcgttcccg
    33841 cgtgaccaac ctcaccgagc tgctgccggg gatcccccat gagcaggttt gcgaggccat
    33901 aaccgaggcc tttttcgccc attatggcga gcgcgtggaa gcggaaatca tctccccgga
    33961 caaaacgcca gacttgccaa acttcgccga aacctttgcc cgccagagta gctgggagtg
    34021 gaacttcggt caggctccgg cattctcgca tctgctggat gaacgcttta gctggggcgg
    34081 cgtggaactg catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg tgtttaccga
    34141 cagcctcaac cccgcgccgc tggaagccct cgccgggcga ctgcaaggct gcctgtaccg
    34201 cgcggatatg ctgcaacaag agtgcgaagc gctgttggtt gacttcccgg accaggaaaa
    34261 agagctacgg gagttgtcga cgtggatagc gggggcggta aggtaactcg ctgattaaaa
    34321 tatccaagga aggaaatatg atcgatctgg aaaaccagga acgggagatt atcaatttaa
    34381 tgttcagcca gcgtatttcc tggctagccg ccgtgcgtat caggcataaa ttatcgcttg
    34441 ctgaagtaag taaaatgtta ggtatctcga ttaattctct aaaacagatc gaaaagacgg
    34501 aacggcttag ctcaaacatc aaaagcaaaa tggcagaaat ttatggttgt ccaccagagt
    34561 tgctgatatg cccttcctgg atgacggctg aacataaatg aatagattcg tgaatagata
    34621 cttgaatgga ttttattttc tatccatatg aaaaataata gtaaatctat aaatcagttg
    34681 ctattgatga atagatacta aaaagccgga gcagtccccc actccggctt aacacgacaa
    34741 tcgcctttca gcgaaacaca attactcttt atcgcccagc agaacggatt ccagtgcgat
    34801 tttgatcatg tcgttgaagg tggtctgacg ctcagcggca gtggtttgct cgtgagtgcg
    34861 gatgtggtca gacacggtgc agatggtcag ggctttcgcg ccaaactctg cagccacgcc
    34921 gtagatacca gccgcttcca tttccacgcc gagaatgccg tatttttcca tcacgtcgaa
    34981 catttcgccg tccggagagt agaacaggtc agcggagaac aggttaccaa cgcgagcatc
    35041 aatgcccagt gctttagctg catctactgc gttacgcacc atgtcgaagt cagcgatagc
    35101 ggcaaagtca tggtctttaa aacggatgcg gttaactttg gaatcggtgc aggcacccat
    35161 accgataacg acgtcgcgca gttttacgtg cggcagaact gcgccacagg aacccacgcg
    35221 gataattttc ttcacgccga aatcggtgat cagttctttg gtgtagatgg agcaggacgg
    35281 gatacccata ccgtgaccca ttacggaaat tttgcggcct ttgtaagtac cggtgaagcc
    35341 cagcataccg cgaacgttgt tcacttcacg ggcatcttca aggaaagttt cagcaatata
    35401 cttcgcacgc agcgggtcgc ctggcatcaa aactacgtca gcgaaatcgc ccatttctgc
    35461 attaatgtgt ggggtagcca ttgttttatc ctttttgtga cataacaaag gcggagtcaa
    35521 cgctccgccc aaatccatca gaacatggct ttgccatatt ccatatcaga agtaccaaaa
    35581 tattttgcca gagtctggcc gatatccgcg aaggtttcac ggtgacccag tgagcccggt
    35641 tttactttcg ggccgtaaac cagtaccgga atgtgttcac gcgtgtggtc agtaccggtc
    35701 caggtcggat cacagccgtg gtcagcggtg aggatcagga tgtcgtcatc gcgcagcaga
    35761 gacatcagct ccggcagacg gcggtcgaac agttccagac ccgcagcata accggcgacg
    35821 tcgcgacggt ggccccagga agagtcgaag tcaacgaagt tggtgaagac gatggtgtta
    35881 tcacccgctt ctttcatctc tttgatggtg gcgtcaaaca gcgcgtccag gccagtcgct
    35941 ttcacttttt tggtgatacc gcagttggcg tagatgtctg caattttacc gaccgaaacc
    36001 acctggccgt gtttttcatc aaccagtttc tgcagcacgg tcggtgctgg cggctcaaca
    36061 gccaggtcgt gacggttacc ggtacgctgg aagttaccgg ctttgtcgcc gataaacgga
    36121 cgagcgataa cacgaccgat attgtagccg ccgttggtca gctcttcacg ggcgatttcg
    36181 cacagttcgt agagtttatc cagaccgaaa gtttcttcat ggcaggcaat ctggaacacg
    36241 gagtcagcgg aggtatagaa aatcggcttg ccggttttca tgtgctcttc gcccagttga
    36301 tccagaatga ccgtaccgga agagtggcag ttaccgaggt aacccggcag attagcgcgt
    36361 tcgaccagtt tatccagcag ctcttgcggg aagctgtttt cgtgatcgga gaaatatccc
    36421 cactcaaaca gaaccgggac accggcaatt tcccagtgac cagacggggt atctttaccg
    36481 gatgacattt cgtgcgccca tgcgtacgcg ccgataactt cagcgttgcc gtccattccc
    36541 gccggaatga aaccggtaga accttcgtgt gctttcgcca gccccagacg ggtcagattt
    36601 ggcagattga gcgggccttt acgaccgtta tcagcttcgc ctttggcaca agcttctgcg
    36661 atatgaccca gggtgtcagc cccgacgtca ccaaagcgtt ctgcatcttc tgtagcgccg
    36721 atgccgaatg agtccagcac cataataaat gcacgtttca tatgttctcc gtactcagtg
    36781 cttcaaattt aaatgatcag atcagtatac cgttattcgc tgatacggcg atagacagtt
    36841 ggtgtgcttt ccggtgcttt atcggcaagt ttaattgccg ctttcaccgc tttcgccgct
    36901 tcctgccagc tgttttcgtc tttcgcgtgg ataaccgcca gcggacgctg gccgtctacc
    36961 tggtcgccca gacgtgccat atcagtaaag ccgacgctgt aatcgatggt gtcagatgcc
    37021 tgacggcgtc cgccgcccat tgcaaccact gccatcccca gcgcgcgggt atccatttca
    37081 ctgacaaaac cttcggtatc agcatagact gctttcgtca gcatcgctgt cggcagatac
    37141 ttcgcgtagt tctcaacgaa gtcggtcggg cctttttgtg ccgctaccat acgaccaaag
    37201 acttctgccg ctttaccgtt gtccagcacc gcctgcaatt tcgcgcgcgc ttcggcgtca
    37261 tctttcgcca gtttgccgga gataagcatc tccacacaca gcgccatcgt gacatcaaac
    37321 agacgcgggt tacgatattc acccgtcagg aactgcaccg cttcacgtac ttcaaccgcg
    37381 ttaccggcac tggaggccag cacctgattc atgtcggtga gcagtgcggt ggtgcgcacg
    37441 cctgcgccgt tagccacgcc aacaatcgct tcggcaaggg cttcagagag ttcgtaggtc
    37501 ggcataaacg cgccgctacc cactttcacg tccatcacca gcgcgtccag accttccgcc
    37561 agtttcttcg ccaggatcga ggcagtgatc agcgggatgg agtccacggt tgcggtaata
    37621 tcacgggtcg cgtagaaacg tttatccgcc ggagccagtg agctggtctg accgataatc
    37681 gccacgccga cgtctttaat aatttcgcgg aaacggttgt catccgggaa aatgtcgaag
    37741 ccagggatgg attccagttt gtcgagcgta ccgccagtat gaccgaggcc gcgaccagag
    37801 atcatcggaa tatagccgcc gcaggctgcg accatcggcc ccaacatcag cgaagtcaca
    37861 tcgccgacgc caccggtgga gtgtttatca acaatcgggc cattcagatg caggcttttc
    37921 cagtcgagaa cggttcctga atctcgcatc gccatggtca gcgagacacg ctcaggcatt
    37981 gtcatatcgt ggaagaaaat ggtcatcgcg agggcggcaa tctgcccttc ggagatagtg
    38041 ttgtcgcgaa taccgttgat aaagaaacga atttcttcat cgctcagcgc atgaccatca
    38101 cgttttttac gaataatttc ttgtgcgaga aacaaggtaa cctcccgagg aaaagagtat
    38161 gaggcggctt ttgccggatg cgtctacgcc ttatccggcc tacgggtaga tacgtctcgt
    38221 aggcctgata agcgcagcgc atcaggcgta aagcatctta cttagtagct gctggcgctc
    38281 ttaccgtcgc cgtgacccag cgctttcagc aggcttgcca gcaggctgga agcaccaaag
    38341 cggtagtgac gcgcatcagc ccagtcagca ccgaacagtt catctgcgat ggcgagatat
    38401 ttctgcgcat cttccgcagt acgcacgccg cccgccggtt tgaaaccaac ggttttttct
    38461 acgcccatat cacggatcac ttccatcatg atgcgcgcgc tttccggcgt cgcgttcaca
    38521 gccactttac cggtagaggt tttgatgaag tccgcacccg ctttgatgga gatttcagac
    38581 gctttacgga tcagcgcttc gtctttcagt tcgccggttt cgatgatcac tttcagcagt
    38641 acattcgctg ccgcgcaagc ctctttacag gctttcacca ggtcaaaacc aacctgctcg
    38701 ttacccgcca tcagcgcgcg gtacgggaac acaacgtcaa cttcatcagc accgtaggcg
    38761 attgccgcac gggtttctgc cagcgcgatt tcgatgtcgt cgttaccgtg tgggaagttg
    38821 gttaccgtag caatacggat ttccggggtg ccctgctctt tcagtgtttt gcgagcaatc
    38881 gggataaagc gaggatagat acagatagcg gcggtattgc cgaccggagt tttggcctga
    38941 tgacacagag caattacttt ctcgtcggtg tcgtcgtcat tcagggtggt caggtccatc
    39001 aatttcagtg cacgcaggct gcttgctttc agatcagtca tttcattctc ctggcttgtc
    39061 gccaataaaa ttcaccttgc gagtttgtta gtattctaac atctactccg caacacactt
    39121 cgatatacat cacaattaag gaaatctact tacaagtttg catcactata atgcgatctg
    39181 gttcaaataa ttcactttca aatgaatgcg tcagtggtgg caaacgcatc aggatcaaag
    39241 tgaacatcac gaaacttctt acaatggcgc atcaccttcg gcataaaagg aaaagaagat
    39301 gcccacttct catgaaaatg cactgcaaca acgttgccag caaattgtca ccagcccagt
    39361 gcttagcccg gagcagaagc gccattttct ggcactggaa gcagaaaaca atctgcctta
    39421 cccacagctt cctgccgaag cccgccgcgc gctggatgaa ggtgtaatct gcgatatgtt
    39481 tgaaggtcat gcgccgtaca aaccgcgcta tgtcttaccc gattacgccc gttttctggc
    39541 gaacggttcc gaatggctgg agctggaagg cgcgaaagat cttgatgacg cactctctct
    39601 gctgaccatt ctttaccacc acgtaccgtc ggtcacatcg atgccggtct acctggggca
    39661 actggatgcg ttgttgcaac cgtatgttat aattctaaca caagacgaga tcgatgttcg
    39721 aataaaacgt ttctggcgtt acctcgacag aaccctgcca gacgccttta tgcacgccaa
    39781 tatcggcccg tctgattcgc ccattacccg cgcgatctta cgtgcagacg cagatttgaa
    39841 gcaggtttca ccgaacctga cctttatcta cgatcctgaa atcacccctg atgacctgct
    39901 gctggaagtg gcgaagaaca tctgtgaatg tagcaaaccg cacatcgcca acggtccggt
    39961 gcatgataaa attttcacaa aagggggcta cgggattgtg agctgttaca actcactgcc
    40021 gctggcgggt ggtggcagca cgctggtacg ccttaacctg aaagccattg ccgagcgcag
    40081 cgaatcgctg gatgacttct ttacgcgcac tctaccgcac tactgccagc agcagatcgc
    40141 catcatcgat gcgcggtgtg aattcctcta tcaacaatca cacttctttg agaatagctt
    40201 cctggtgaaa gaagggctga ttaaccctga acgttttgtg ccaatgtttg gcatgtatgg
    40261 gctggcggaa gcggttaact tgctgtgtga aaaagaaggg attgccgcgc gctacggtaa
    40321 agaagccgcc gcaaatgaag taggttatcg catcagtgca cagctggcgg agtttgtcgc
    40381 caataccccc gtgaaatatg gctggcaaaa acgcgccatg ttacacgcac agtcggggat
    40441 cagttccgat atcggcacca cgccgggcgc gcgtttgccg tatggcgatg agccagatcc
    40501 gatcacccat ctgcaaactg tcgcccgcat catgcttatt attattctgg catcagcgac
    40561 attctgacgc tcgacgaaac catcaaacgt aacccgcagg cgttggtaca gctttgcctc
    40621 ggtgccttta aagccggaat gcgtgaattt accgccaatg tcagcggtaa cgatctggtt
    40681 cgcgttaccg gttatatggt gcgtttgtcg gatttagaaa aatatcgcgc cgaaggttca
    40741 cgcaccaaca ccacctggct gggcgaagaa gccgcacgca acactcgtat tctggaacgc
    40801 cagccgcgcg tgataagcca tgaacagcag atgcgcttta gtcagtaaga ttatcccctt
    40861 ctcctgcgtt gacgggccag gcagtcgtct ggctctgttt ttgcagggct gcaatctgcg
    40921 ctgcaaaaac tgtcacaatc cgtggacgat gggacgttgc aatgactgtg gggagtgcgt
    40981 gccacagtgt ccgcatcagg cgttgcagat tgttgacggc aaagtggtgt ggaacgctgt
    41041 ggtctgcgag caatgtgata cctgcctgaa gaggtgtccg caacatgcca cgccaatggc
    41101 gcaatccatg agcgtggatg aggtgcttag ccatgtccgc aaagcagtgc tgtttatcga
    41161 agggataacg gtgagtggcg gcgaagccac gacccaactg ccgtttgtag tggcgctgtt
    41221 tactgctatc aaaaacgatc cgcaactgcg ccacctcacc tgcctggtgg acagtaacgg
    41281 tatgttgagc gaaaccggct gggaaaaatt gctcccggtg tgcgacggcg caatgctcga
    41341 tctcaaagcg tgggggagcg aatgtcatca gcaattaacc ggacgcgata atcagcagat
    41401 taagcgcagc atctgtttgc tggcagagcg cggtaagctg gcggaactgc gtttgctggt
    41461 aatccctggc caagtggatt atttgcaaca catcgaagaa ctggcggcgt ttatcaaggg
    41521 agtcggcgat gttccggtac gcctgaacgc gtttcatgcc cacggcgtgt atggcgaggc
    41581 gcaaagctgg gcgagcgcca cgccggaaga cgttgagccg ttggctgatg cgttacggga
    41641 gcgcggggtg agccggttaa tatttccggc gctgtatttg tgacatgccg gatacgacct
    41701 acggttcggc acagacttgt aggcctgata agacgcgtca agcgtcgcat caggcattgt
    41761 gcgccaactg ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg
    41821 taggcatgat aagacgcgtt aagcgtcgca tcaggcatta tgcgccgact gccggatgct
    41881 gcctacgaag ggcaaactac ccacgaacgt taaacaccgc atacgtgtta ttaagcaaca
    41941 cttcggcaat ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgcg
    42001 cagcctgttc cgggcggtta ggctgcccct gaaaaccgtt gagcggcata tccggcgcgt
    42061 cggtttccag caacaacgat gccagcggta attttgcgat gacaacgcgg gttttactgg
    42121 cgcgtggata ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct
    42181 cggcctgttg caggctgccg gaaaaaccgt gaaccacgcc agtgcgcgat aaatcgtggc
    42241 gtttaagatg catcgccagt ttgtcgtgtg tgcgccgtga atgcaggatc accggcagat
    42301 cgtagcgttt cgccagtttc agttgttcgt cgagaaacca ctgctgcctc tcaaattgcg
    42361 gatcgtcgcc gaagagatcc agaccgctct cccccaccgc caccaccttc gccggacgcc
    42421 tttccagcgc ctgctgtagc tgatcaagag acacatcgct atgtttttcc aacataccag
    42481 gatgcaagcc caatgcggca tacagcggtt gataattttc cgctaacgcc tgcacgcggg
    42541 caaaattttc cgcctcggtt gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc
    42601 gttgcaggct ggcctcttca tcgccaataa aaggcgggaa atcaaaatgg cagtgggtgt
    42661 cgataaaacg gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg
    42721 agcgtcgctc acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaatgacaat
    42781 cgattccggc gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag
    42841 cttgccaacc gtggcgagga aataacgacc gcataaacgc ccaagtttat agtcttcacg
    42901 cagcgccgga atccgactgc caagcgcgat actatgtaat ggcttcggcg gataaatttc
    42961 gaatatccgc agcttgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt
    43021 ttcatgatgc tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc
    43081 catgcgtttg aaccactgcg gcgtgtagta catttgtgac ggcacagtgc ggatgacgac
    43141 caacgtttta gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc
    43201 atccaggtag ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact
    43261 ggaggcgcga atcacatcca gccagttttg tttggttggt aaaaagtaat tcggcgcgta
    43321 gtcatcctga cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc
    43381 ggtatccatt tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat
    43441 aagatttcct ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg
    43501 catgatgact ttgcgcgcgt aaccgggctg attgcagata tacgccgaga ggttctgcgc
    43561 cccggcagat gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc
    43621 cagcacgcca gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg
    43681 tccaggctgg aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg
    43741 ctgccccacc tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat
    43801 tattcgctaa aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat
    43861 ggtctgaaat ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc
    43921 cgacgacaaa gacaatttta gctgcgcctg cagcggtacc ggccagacca ccaaacccaa
    43981 gtgcggcggc gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct
    44041 cctttaccat agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt
    44101 tttagtgtgg tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt
    44161 acttagtttt cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt
    44221 cagcacggtc actttgtgcc tgagaatcta cgataccaga gagctgaacc acgccatcgg
    44281 tggtttcaac tttcacatga cgggaaggga cgatatcgtc cgccagcaat ttggctttga
    44341 tttcactggt ggtggcggtg tcacccgcgt agcccttcac agagccttct ttagcgtcgc
    44401 gaacgtgcag tttgtcgctg acagaggtca ccccttcaac acctttcgcc actttcactg
    44461 cctcttcggc ctgggcctgg ctttcaacga aaccgctcag ggtcacgact ttttgatcgg
    44521 ttttgacaga gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt
    44581 tcgcggtgat ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt
    44641 tttgccctgc gctttcattg gtagtctgcg cgttgttttc cgcgtaggca gagccggtcg
    44701 cgacggcaga ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag
    44761 tcatcgattt attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt
    44821 gctgaatagg gagagacttc accctctaca gaagtcaatg gtcgccatca caaaagcgat
    44881 gagtgatgaa taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca
    44941 atcgacggct ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg
    45001 tcatcacttt gttaaatata gatcacaatt ttgaaaccgc tcgggatatc agcgaaaaca
    45061 taagcaaaag tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga
    45121 caggacgcag cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa
    45181 ctgaacgtcc ggataacgtt cctgtgccag gcgcaggttg accatgctgg tagcgatgta
    45241 agcgaggtta tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc
    45301 gaatttcttc gcgtctgcac attctaccca gcgggcagtg gcaacgttga ctgactcata
    45361 cactgcttca acgttgtatt cgctcttcag gcgcgctacc accacatcaa actgcagcac
    45421 accaaccgca ccaacgatca gatcgttgtt ggagattgga cggaacacct gcaccgcgcc
    45481 ctcttcggaa agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag
    45541 gcggatacga cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc
    45601 accctgggta aaggtgtcgc cgatctgaat ggtgccgtgg ttgtgcaggc cgaggatatc
    45661 gcccggatac gcttcttcaa cgtgcgaacg gtcacccgcc ataaaggtca gcgcgtcgga
    45721 gatcaccaca tctttcgcag tgcgcacctg gcgcagcttc atgccttttt catatttacc
    45781 ggacaccaca cgcataaacg ccacgcggtc gcggtgtttc gggtccatgt tggcctgaat
    45841 tttaaatacg aagccggtaa atttatcttc gctcgcttct acggtacggg tatcggtctg
    45901 acgcggcatc ggcgcaggcg cccactccac caggccatcc agcatatgat caacgccgaa
    45961 gttacccagc gcagtaccga agaataccgg agtgatttcg cccgcaagga acagctcttt
    46021 gtcgaactcg ttagacgcgc ctttcaccag ttccagttcg tcacgcagct gctgtgccag
    46081 atcttcacca accgcagcat cgagatccgg gttattcagc cctttaacaa tgcggacttc
    46141 ctgaatggtg tgccctttac cgctctgata gagataggtt tcatctttat aaaggtggta
    46201 aacgccttta aacagcttgc cgcagccaat tggccaggtg atcggtgcgc agccaatttt
    46261 cagctcgttc tcaacttcat cgagcaattc catcgggtcg cggatatcac ggtcaagttt
    46321 gttcataaag gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt
    46381 acgatcttca acaccttttg cggcgtcgat aaccatcagg cagcagtcca ccgccgtcag
    46441 ggtacgatag gtatcttccg agaagtcttc gtgccccggg gtgtcgagca agttaaccag
    46501 gcaatcgtga tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc
    46561 catctccatc cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccggc
    46621 ggtctgaatg gcctgtccga acagcagcac cttctcggtg atggtagtct taccggcgtc
    46681 cgggtgagaa ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa
    46741 cgtcataatt ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa
    46801 ttgcggctat tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc
    46861 cagttcgtgc aacgaagaaa cggtccaggt gggcgcgatg ccttctggtt gctcgcgatg
    46921 gtgtgcattc agccagcagg tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc
    46981 ggcagtgtcg ccaaccatca gcacgcgtga acggtcagga ttgcccgcct gttccagcgc
    47041 ataatcgaaa attttcttat tcggtttggc aacgccaact tcttcggaaa tcaccagcaa
    47101 atcgaagtaa tcacgcaggc ccgtgcgttc cagacgcacc tgttgcaagg cactaaagcc
    47161 gttggtgatg atgccgattt tggcgttgcc acgaatggcg ttaagcagag aaaccgcgcc
    47221 cggcagcggc gtgcagattt ccgccatcgc attaataaag gcttcgttga gtttacctgg
    47281 ctcgacgttc agccgttcgg cccagctctc aaaccgcccg tgctgaagct gtaatgaagt
    47341 gatcgcgccg ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa
    47401 atcttcagcg gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga
    47461 gtcaaaggta aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc
    47521 accttgtctt acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg
    47581 gtggggtagt aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca
    47641 atggcggcag catttgaggc acggacttcc agccatagtg tcgccacgcc gcgtttttcc
    47701 agttcgtcga tcagatgttc cagcagcgcc cttcccaatc cctgacgctg atagtcagga
    47761 tcgaccgcaa tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc
    47821 gccattttgc cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca
    47881 aaggtttttt cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt
    47941 aaatcagtcg tttcgaggga agaaatcgtg ttcatatgtg caaatttgtt gccataacgc
    48001 ggcgcgtgcc gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc
    48061 ttccagtgat agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg
    48121 cagcatcgcg attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc
    48181 gcgcagaaca tcgctcacta aaggatcagt cagggcggga agatcgtttg ccaccatcac
    48241 cagacggacg tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg
    48301 cagcgaccac tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc
    48361 gaatcgctcc tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata
    48421 tggctataat cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca
    48481 agtgaagtct tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga
    48541 gacttacagg atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag
    48601 caattccagc attggcaggt gttaagccgc cagatggggg ataacgcccg ttttagtctg
    48661 gtcgccacgg cggatgatgt cgcagattgc gatacgctga tttactactg gccgaagaac
    48721 aaaccggaag cccagttcca gttgatgaat ttactttctc tgcttccggt ggggacggat
    48781 atttttgttg ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggctgat
    48841 tatgcgccgt tgaataaagt cgacagcgcc cgtcgctgtg gcctctattt tggtcgtctg
    48901 gaaaaacagc cggtatttga tgccgataaa ttctggggcg aatacagcgt cgatggcctg
    48961 acggtcaaaa cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg
    49021 ctgctctcga cgttaacccc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg
    49081 ggcgtgcttt cggttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat
    49141 gtctctgcgc cagcggtaga agccagccgc gcaacacttg cggccaacgg tgttgaaggt
    49201 gaagtctttg ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc
    49261 aacccgccgt tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgc
    49321 ggtgcggtgc gtcatcttaa tagcggcggc gagctgcgaa ttgtagcgaa cgccttcctg
    49381 ccttatccgg acgtgctgga tgagacattt ggcttccacg aagtgatcgc gcaaacaggg
    49441 cgcttcaagg tgtatcgcgc cattatgacc cgccaggcga agaaaggtta attatctcgc
    49501 cgaataccgt gtcggatgcg gcgtgaacgc cttatccgac ttacaaaacc tgcacgttag
    49561 tctcctgatg gacatttttc cagcaattac acatcaggcg ataattaact attgacgaaa
    49621 agctgaaaac cactagaatg cgcctccgtg gtagcaattc tttttaagaa ttgatggtat
    49681 gcgaaggtgg cggaattggt agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg
    49741 ggggttcaag tcccccccct cgcaccacga ctttaaagaa ttgaactaaa aattcaaaaa
    49801 gcagtatttc ggcgagtagc gcagcttggt agcgcaactg gtttgggacc agtgggtcgg
    49861 aggttcgaat cctctctcgc cgaccaattt tgaaccccgc ttcggcgggg ttttttgttt
    49921 tctgtgcatt tcgtcaccct cccttcgcaa taaacgcccg taataactca ttgccccacg
    49981 gtatgatttc gcccttaacg tattgaagga tgacttcagg caaggagcga ccatgctgca
    50041 acaggttcca acgcgtgctt ttcatgtgat ggcgaaaccg agcggttccg attgtaatct
    50101 gaactgtgac tactgttttt atctcgaaaa acaatccctt taccgcgaaa agccagtcac
    50161 gcatatggac gatgacacgc tggaagcgta tgtccgtcac tatatcgctg ccagcgaacc
    50221 gcaaaacgaa gtggctttta cctggcaggg cggcgaacca acgctactcg ggctggagtt
    50281 ttaccgccgt gccgtggcgc tacaggcgaa atatggtgct ggcaggaaga taagtaacag
    50341 cttccagact aacggcgtac tgctcgatga tgaatggtgt gcatttctgg cagaaaatca
    50401 ttttcttgtt gggttatcgc tggatggtcc ggctgagatc cacaatcaat atcgcgtgac
    50461 aaaaggcggc agacccacgc ataaactggt gatgcgtgcc ctgacgctcc tgcaaaaaca
    50521 tcatgtcgac tataacgtgc tggtctgcgt caaccgcacc agcgcgcagc aaccgttaca
    50581 ggtttatgat tttttgtgcg atgcgggagt cgaattcatc cagtttattc cggtggtcga
    50641 gcgcctggct gatgaaacga ctgcccgcga aggactgaaa ctacatgcgc ctggtgatat
    50701 tcagggggaa ctgacggaat ggtctgtgcg ccccgatgaa tttggtgaat ttctggtggc
    50761 gatattcgac cactggatta aacgcgacgt cggcaagatt ttcgtgatga atatcgaatg
    50821 ggcgtttgcc aattttgtcg gtgcgccggg tgcggtttgc catcatcagc caacctgtgg
    50881 gcgctcggtg attgttgagc acaatggcga cgtttacgcc tgcgatcact atgtttatcc
    50941 gcaatatcga ctggggaata tgcaccagca aacaattgca gaaatgatcg attccccgca
    51001 acagcaggtg tttggtgaag ataaatttaa gcaattaccg gcgcagtgtc gcagttgtaa
    51061 cgtgttaaaa gcatgttggg gaggctgccc gaaacaccgc ttcatgctcg atgccagcgg
    51121 caaaccggga ctgaattatt tgtgtgccgg gtatcagcgt tatttccgcc atctaccgcc
    51181 atatcttaaa gcaatggctg atttgctggc gcacggtcgc ccggccagcg acattatgca
    51241 ggcgcatttg ctggtggtga gtaagtaaaa aagcggccat tttgtggtcg cctgcggttg
    51301 attgccggat gcggcgtaaa cgccttatcc ggcctacatg atcgtgcaaa ttcaataaat
    51361 tgcagcgttc tgtaggctgg ataagatgcg tcagcatcgc atccggcaaa ggcagatctc
    51421 agcgatagcg ccggcttaat cagatttaat ctgcgcgcgt ggtggatact ttttcaggat
    51481 ctccatatac gcgtgcattt cggtctgtag cggtacaccc atcggaatat ggcgcacgcc
    51541 gatagagtcg ctctcctgcg gatcggtgta gaggttaaac accgacgatc cggctgtttg
    51601 cattactgtg ccggtgaatc caccctgata tccgctctgg gtataagcgt aaggttgctg
    51661 aatcaggacg tgatacttga attcatccat acgcacagca gcgagtttac cgttgaggaa
    51721 gtagtgctcg gccttacggt tagactgacc atttgttccc aggaagaaag atgtctggtc
    51781 cacaccatcg ataaaggtgg ttttcggcac taaattcgcc actttcgcac caggatgccc
    51841 cgccagatcc agtgctgttg ggaagagatc tgccagatca acaataccgt cagatttacg
    51901 cggttggatc atgcctttcc agtaaacgaa agtcggtacg cgaacgccgc cttcccaggt
    51961 agatcctttc gcaccacgga acggtgtgcg tccgtgcggc ggtacttcgg cttccggtcc
    52021 gttatcggag gtaaagacaa tcagcgtgtt atcaagctga ccgtttttct ccagtgcttt
    52081 atacagatta gcgaacacat cgttcatttc caccatgcag tcgccatacg aggtgcgtgc
    52141 cggagagcta cccgcatatt tggcgttcgg gtagttatcg aagtgacagc cgcgagtgcc
    52201 gtaatagagg aagaaaggct tatcactctt cgccatcttg tcgaggaact taacgccata
    52261 ttccatccag cgttgatcca gatcttccat atatttcggc gtaatgtcgg caatggcctg
    52321 ttgttcgccg ccgcgcaccg catgaacgtc atctttgcta aatggtaatt gcttgatgta
    52381 ttcagaacgg tccggactca gggccacttc cggattgacg tgaacgtcgc gccattcggt
    52441 gtacatatca gacaccgagt taaagccacg gaaatcatca aagccaacgt tctgcggctg
    52501 cgactctttg ttttccccca tatgccattt cccgatggcc tgagtgacgt agccctgatc
    52561 gtgcagcaac tgcggcagcg tggttaaccc ttgcagcccg cccggttgcc cgtacattgg
    52621 cggcatcaga atgccgtggt ggatggagta ttgtccggtg agaatggtgg cgcgggttgg
    52681 ggaagagctt ggttgagaat acgccgaagt taaaatcagc ccctggctgg caacggcgtc
    52741 gatatctggt gtagggttac ccaccgccac gccgccaccg ttaaaaccga cgtccatcca
    52801 gcccacatcg tccagcaaga aaacaaccac attcggtttc ttaccggttt ttttctcaag
    52861 ttctgccagc ttctgctggg gttctttatc ctgcgccgga tgctgcatta ctggcatcat
    52921 gttgtcggca atagtggtcg ccggtttaac cagatactgg tttgggtgat cgtatccggc
    52981 aaagcctttg cgcgcggtgg cagttgacgg ggtatctgct gcgctggcca tgagaggaag
    53041 agcggcggcg acagcaacaa caagaagttt gggtgaaaac gaaaattcca tgcaaaatgc
    53101 tccggtttca tgtcaacaaa atgatgacgt aattaagcat tgataattga gatccct
//
LOCUS       DAZFBR010000019        51696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFBR010000019 DAZFBR010000000
VERSION     DAZFBR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.019"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            15..1259
                     /gene="rtcB"
                     /locus_tag="ACHJZ2_001306"
     CDS             15..1259
                     /gene="rtcB"
                     /locus_tag="ACHJZ2_001306"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO4567454.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTLGTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLE
                     TLPSRDLAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQ
                     TLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGN
                     EESFCSCSHGAGRVMSRTKAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAV
                     MAAQSDLVEVIYTLRQVVCVKG"
     gene            1263..2291
                     /gene="rtcA"
                     /locus_tag="ACHJZ2_001307"
     CDS             1263..2291
                     /gene="rtcA"
                     /locus_tag="ACHJZ2_001307"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO4567455.1"
                     /translation="MKRMIALDGAQGEGGGQIMRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(2341..2826)
                     /locus_tag="ACHJZ2_001308"
     CDS             complement(2341..2826)
                     /locus_tag="ACHJZ2_001308"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4567456.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(2817..3095)
                     /locus_tag="ACHJZ2_001309"
     CDS             complement(2817..3095)
                     /locus_tag="ACHJZ2_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO4567457.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            complement(3162..5867)
                     /gene="malT"
                     /locus_tag="ACHJZ2_001310"
     CDS             complement(3162..5867)
                     /gene="malT"
                     /locus_tag="ACHJZ2_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO4567458.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            6478..8871
                     /gene="malP"
                     /locus_tag="ACHJZ2_001311"
     CDS             6478..8871
                     /gene="malP"
                     /locus_tag="ACHJZ2_001311"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO4567459.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8881..10965
                     /gene="malQ"
                     /locus_tag="ACHJZ2_001312"
     CDS             8881..10965
                     /gene="malQ"
                     /locus_tag="ACHJZ2_001312"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO4567460.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(11010..12326)
                     /gene="gntT"
                     /locus_tag="ACHJZ2_001313"
     CDS             complement(11010..12326)
                     /gene="gntT"
                     /locus_tag="ACHJZ2_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4567461.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(12687..13262)
                     /gene="nfuA"
                     /locus_tag="ACHJZ2_001314"
     CDS             complement(12687..13262)
                     /gene="nfuA"
                     /locus_tag="ACHJZ2_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO4567462.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(13321..14004)
                     /gene="gntX"
                     /locus_tag="ACHJZ2_001315"
     CDS             complement(13321..14004)
                     /gene="gntX"
                     /locus_tag="ACHJZ2_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO4567463.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            14042..14812
                     /gene="bioH"
                     /locus_tag="ACHJZ2_001316"
     CDS             14042..14812
                     /gene="bioH"
                     /locus_tag="ACHJZ2_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO4567464.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            complement(14839..15717)
                     /gene="rpnA"
                     /locus_tag="ACHJZ2_001317"
     CDS             complement(14839..15717)
                     /gene="rpnA"
                     /locus_tag="ACHJZ2_001317"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO4567465.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(15920..16156)
                     /gene="feoC"
                     /locus_tag="ACHJZ2_001318"
     CDS             complement(15920..16156)
                     /gene="feoC"
                     /locus_tag="ACHJZ2_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO4567466.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(16156..18477)
                     /gene="feoB"
                     /locus_tag="ACHJZ2_001319"
     CDS             complement(16156..18477)
                     /gene="feoB"
                     /locus_tag="ACHJZ2_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO4567467.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(18494..18721)
                     /gene="feoA"
                     /locus_tag="ACHJZ2_001320"
     CDS             complement(18494..18721)
                     /gene="feoA"
                     /locus_tag="ACHJZ2_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO4567468.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(19159..21480)
                     /gene="yhgF"
                     /locus_tag="ACHJZ2_001321"
     CDS             complement(19159..21480)
                     /gene="yhgF"
                     /locus_tag="ACHJZ2_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO4567469.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(21577..22053)
                     /gene="greB"
                     /locus_tag="ACHJZ2_001322"
     CDS             complement(21577..22053)
                     /gene="greB"
                     /locus_tag="ACHJZ2_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO4567470.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            22281..23000
                     /gene="ompR"
                     /locus_tag="ACHJZ2_001323"
     CDS             22281..23000
                     /gene="ompR"
                     /locus_tag="ACHJZ2_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO4567471.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            22997..24349
                     /gene="envZ"
                     /locus_tag="ACHJZ2_001324"
     CDS             22997..24349
                     /gene="envZ"
                     /locus_tag="ACHJZ2_001324"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO4567472.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(24425..26047)
                     /gene="pckA"
                     /locus_tag="ACHJZ2_001325"
     CDS             complement(24425..26047)
                     /gene="pckA"
                     /locus_tag="ACHJZ2_001325"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO4567473.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            26426..28150
                     /gene="yhgE"
                     /locus_tag="ACHJZ2_001326"
     CDS             26426..28150
                     /gene="yhgE"
                     /locus_tag="ACHJZ2_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO4567474.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            complement(28213..29091)
                     /gene="hslO"
                     /locus_tag="ACHJZ2_001327"
     CDS             complement(28213..29091)
                     /gene="hslO"
                     /locus_tag="ACHJZ2_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO4567475.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQLLENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(29116..29517)
                     /gene="hslR"
                     /locus_tag="ACHJZ2_001328"
     CDS             complement(29116..29517)
                     /gene="hslR"
                     /locus_tag="ACHJZ2_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO4567476.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(29528..30196)
                     /gene="yrfG"
                     /locus_tag="ACHJZ2_001329"
     CDS             complement(29528..30196)
                     /gene="yrfG"
                     /locus_tag="ACHJZ2_001329"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO4567477.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(30261..32396)
                     /gene="igaA"
                     /locus_tag="ACHJZ2_001330"
     CDS             complement(30261..32396)
                     /gene="igaA"
                     /locus_tag="ACHJZ2_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO4567478.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            32716..33276
                     /gene="nudE"
                     /locus_tag="ACHJZ2_001331"
     CDS             32716..33276
                     /gene="nudE"
                     /locus_tag="ACHJZ2_001331"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO4567479.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(33441..35993)
                     /gene="mrcA"
                     /locus_tag="ACHJZ2_001332"
     CDS             complement(33441..35993)
                     /gene="mrcA"
                     /locus_tag="ACHJZ2_001332"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO4567480.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            36113..36907
                     /gene="hofM"
                     /locus_tag="ACHJZ2_001333"
     CDS             36113..36907
                     /gene="hofM"
                     /locus_tag="ACHJZ2_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO4567481.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            36891..37430
                     /gene="hofN"
                     /locus_tag="ACHJZ2_001334"
     CDS             36891..37430
                     /gene="hofN"
                     /locus_tag="ACHJZ2_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO4567482.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            37414..37854
                     /gene="hofO"
                     /locus_tag="ACHJZ2_001335"
     CDS             37414..37854
                     /gene="hofO"
                     /locus_tag="ACHJZ2_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO4567483.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            37844..38248
                     /gene="hofP"
                     /locus_tag="ACHJZ2_001336"
     CDS             37844..38248
                     /gene="hofP"
                     /locus_tag="ACHJZ2_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO4567484.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            38160..39398
                     /gene="hofQ"
                     /locus_tag="ACHJZ2_001337"
     CDS             38160..39398
                     /gene="hofQ"
                     /locus_tag="ACHJZ2_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO4567485.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            39799..40320
                     /gene="aroK"
                     /locus_tag="ACHJZ2_001338"
     CDS             39799..40320
                     /gene="aroK"
                     /locus_tag="ACHJZ2_001338"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO4567486.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            40377..41465
                     /gene="aroB"
                     /locus_tag="ACHJZ2_001339"
     CDS             40377..41465
                     /gene="aroB"
                     /locus_tag="ACHJZ2_001339"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO4567487.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            41557..42843
                     /gene="damX"
                     /locus_tag="ACHJZ2_001340"
     CDS             41557..42843
                     /gene="damX"
                     /locus_tag="ACHJZ2_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO4567488.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            42950..43786
                     /gene="dam"
                     /locus_tag="ACHJZ2_001341"
     CDS             42950..43786
                     /gene="dam"
                     /locus_tag="ACHJZ2_001341"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4567489.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            43804..44481
                     /gene="rpe"
                     /locus_tag="ACHJZ2_001342"
     CDS             43804..44481
                     /gene="rpe"
                     /locus_tag="ACHJZ2_001342"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO4567490.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            44474..45232
                     /gene="gph"
                     /locus_tag="ACHJZ2_001343"
     CDS             44474..45232
                     /gene="gph"
                     /locus_tag="ACHJZ2_001343"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO4567491.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            45225..46229
                     /gene="trpS"
                     /locus_tag="ACHJZ2_001344"
     CDS             45225..46229
                     /gene="trpS"
                     /locus_tag="ACHJZ2_001344"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO4567492.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(46360..47091)
                     /gene="frlR"
                     /locus_tag="ACHJZ2_001345"
                     /gene_synonym="yhfR"
     CDS             complement(46360..47091)
                     /gene="frlR"
                     /locus_tag="ACHJZ2_001345"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO4567493.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(47191..47976)
                     /gene="frlD"
                     /locus_tag="ACHJZ2_001346"
                     /pseudo
     CDS             complement(47191..47976)
                     /gene="frlD"
                     /locus_tag="ACHJZ2_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            complement(47976..48803)
                     /gene="frlC"
                     /locus_tag="ACHJZ2_001347"
     CDS             complement(47976..48803)
                     /gene="frlC"
                     /locus_tag="ACHJZ2_001347"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO4567494.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            complement(48854..49876)
                     /gene="frlB"
                     /locus_tag="ACHJZ2_001348"
     CDS             complement(48854..49876)
                     /gene="frlB"
                     /locus_tag="ACHJZ2_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO4567495.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(49897..51234)
                     /gene="frlA"
                     /locus_tag="ACHJZ2_001349"
     CDS             complement(49897..51234)
                     /gene="frlA"
                     /locus_tag="ACHJZ2_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO4567496.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(51530..>51696)
                     /gene="yhfL"
                     /locus_tag="ACHJZ2_001350"
     CDS             complement(51530..>51696)
                     /gene="yhfL"
                     /locus_tag="ACHJZ2_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO4567497.1"
                     /translation="NKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISK
                     IIGGCGPTAQ"
BASE COUNT        12208 a        13133 c        14122 g        12233 t
ORIGIN      
        1 aagagaaaaa caaaatgaat tacgaattac tgaccactga aaatgccccg gtaaaaatgt
       61 ggaccaaagg cgtgccggta gaagccgatg cgcgtcagca acttattaat acggcgaaga
      121 tgccgtttat tttcaaacat attgcggtaa tgcctgatgt acacctgggt aaaggttcca
      181 ccattggtag cgtgatcccg accaaagggg cgattattcc ggcggcggtg ggcgtggata
      241 ttggctgtgg aatgaacgca ctacgtaccg ctttaacggc ggctgacctg ccggaaaacc
      301 tggcggagct gcgtcaggcg attgaaactg cggtgccgca cgggcgtacc actggccgtt
      361 gtaaacgtga taaaggcgca tgggaaaacc cgcctgttaa cgtcgatgcg aaatgggctg
      421 agctggaggc cggttatcag tggttaacgc aaaaatatcc gcgtttcctt aacactaaca
      481 actataaaca cctgggaacg ctgggaacgc tgggaacgct gggaacgggt aaccacttta
      541 ttgaaatctg ccttgatgag tcggaccagg tgtggattat gctgcactcc ggttcacgcg
      601 gaattggtaa tgccatcggg acttacttta tcgatctggc gcaaaaagag atgcaggaaa
      661 cgcttgaaac attgccgtcg cgtgatctgg cgtactttat ggaaggtacg gaatactttg
      721 atgattacct gaaagccgtg gcctgggcgc agctttttgc cagccttaac cgcgatgtga
      781 tgatggaaaa cgtagtaacg gcgttgcaga gcattacgca gaaaacggtc agacagccac
      841 aaacgctggc gatggaagag atcaactgtc accacaacta tgtgcaaaaa gaacagcact
      901 ttggtgaaga gatctacgtg acgcgtaaag gcgcggtgtc tgcgcgtgct ggtcaatatg
      961 gaattattcc agggtcgatg ggagcgaaaa gctttatcgt ccgtgggctg ggaaatgaag
     1021 agtcgttctg ttcgtgcagc cacggtgccg ggcgggttat gagccgtact aaagcgaaaa
     1081 aaatgttcag cgtggaagat caaattcgtg ccaccgtgca tgtggaatgc cgtaaagatg
     1141 ccgaagtgat cgacgaaatc ccgatggcgt ataaagatat tgatgcggtg atggcggcac
     1201 aaagcgatct ggtggaagtt atctacaccc tacgtcaggt ggtgtgcgta aaaggataaa
     1261 tgatgaaaag gatgattgcg ctggatggcg cacagggcga aggcggcggg cagatcatgc
     1321 gctcggcgct gagtctgtcg atgataaccg gccagccatt taccatcacc ggcattcgtg
     1381 ccgggcgggc aaaaccggga ctgttgcgcc agcatctgac ggcggtaaag gcggcgacgg
     1441 aaatttgcgg ggcgacggtg gaaggcgcgg agctggggtc gcagcgtctg gtcttccggc
     1501 ccggcaccgt gcgtggcggc gattatcgct ttgctatcgg tagcgcagga agttgtacgc
     1561 tggtgctgca aacggtgctg cccgcgctgt ggtttgccga tggaccttcg cgtgttgaag
     1621 tgagcggcgg taccgataac ccgtcggccc cgcctgcgga ttttatccgc cgggtgttgg
     1681 agccgctgct ggcgaaaata ggaattcatc agcaaaccac gctgttacgt cacggttttt
     1741 atcctgccgg aggcggcgtg gtggcaacgg aagtctcgcc ggtggcatcg tttaacacct
     1801 tgcaacttgg cgagcgcggg aacattgtgc agatgcgtgg agaggtgtta ttagctggcg
     1861 tgccgcggca tgtcgctgag cgtgaaatcg ctacgctggc ggcaagtttt tccctgcatg
     1921 agcagaatat tcataacctg ccgcgcgacc aggggccggg taataccgtc tcgcttgaag
     1981 tcgaaagtga aaatatcacc gaacgctttt ttgtcgtcgg tgaaaagcgc gtcagtgccg
     2041 aggtggttgc ggcacagttg gtgaaagagg tgaaacgcta cctggcaagc ccggcggcag
     2101 tgggggaata tcttgctgac cagctagtgt tgccaatggc gctggcgggc gcgggacaat
     2161 ttacggtcgc ccatccctca tgccatctgc tgaccaatat cgccgtggtg gagcgtttct
     2221 tgccggtgcg gtttactctg gctgaaacag atggcgtaac gcgggtgatg ataaccaaac
     2281 tcactgattg aaatccctcc cggatcagac tcatcagtgt gactatccgg tagaggtaaa
     2341 ttactccaga gtaatgggat acagtaaggt aagtgactgg aatggtgaag gtacaaaggc
     2401 atacttcaga tagaaattgc gtactgaatc gtctaaggca tgaacaatga gcgcacgaac
     2461 ccctacctgt tgagctatgg acattgtgcg atagactgca tctttcaaca atgcgacgcc
     2521 caacccttta cctgcccagg cttgatcaat tgccagtctg cctaatacga caacaggcaa
     2581 cgattcggga gcattacgtc gcatggctcc aggggccaaa ttacgttgaa tactgcctgt
     2641 agaaaggcag taatagccga ttacctggcg ggtattggca atacagataa cgtaaacacg
     2701 agacagaccg gagctgtgat tttttaacgc tttctttttt agccattcat tcatcccgtg
     2761 atcggaacag caaaagtcag ccagattatg ttcagacgtt aggggagtag gagccgttat
     2821 tcccatggcg atttacgatt catcaacgcg ttaattttgg cctgacgctc agcagtgatg
     2881 ggggcgtcca gggcagcgag aaacgcatcg tattgctcgt catcaaggat gaatagtcgt
     2941 tgatctaata agatgtcttg cgcttcacgg catgcggcat ccagcataaa atcagtgcgc
     3001 gattttgcga ccagattcgc tgccatatca atcaggtcac gctgtgaggc tttcgctctg
     3061 atattgattg gcgcttcttt gctttccggt ttcatattca tgagcctgta gtgcgatttt
     3121 atatacagta taaagagtgt acataaaaac gctatacaaa ctcacacccc ataacccatc
     3181 atcttcagca gtttctgcgc gtgctgcacg gcagcctgac ggtgggcgac gccgagtttt
     3241 tggtacagat tgcggatatg cgtcttgatg gtggttgccg ccacttccag ttcacccgct
     3301 atttgttcgt tgctgtaacc ggagtaaatc agccccagga cctgccattc acgttgggtc
     3361 aacgggctgg tgcggatcag ttctggcact tccggatgat ttagcagacg ttcaacgaaa
     3421 ttctcatcga aatgggcgaa tttatgccga tgatgttgat tgatttctcg tagaatacgc
     3481 tgtgcgcgat gctgttccag ttccggcagc gtattgagtt gaatcagctg gcgcagttgt
     3541 tgcgccatcg cttcgccttc gatgacaaaa tgactgataa atccggtgcg attcgccagt
     3601 tttaatgcgt ccagcaacac gcgctgggcg tcacttttac gtccggcctg ccagtacagt
     3661 tgatttagca gcaacaggtt acggttgaga tcgctcatca accgcagact gcgggcattt
     3721 tcattgagtt cttcgaggac aatttccgca ggttcaaact cgcccagcaa gatttgtgcg
     3781 cgggcaatgt tgcgccattg accttgcagg aagtggttgt tcgcaaactc cggtttagcc
     3841 gtatgacgca accagttggc agcggcggct ttatcgccgg tcatttgcca gtaaatcacc
     3901 cggactttgt tggcgttaga gatccagtcg ctgtgatatt tgccattccc cagcaggttt
     3961 tccagacggt tcagctggct acgggcgtta tctaaatcac cacgggccag cgagcattga
     4021 atcaacattg ccaggcactg aagctgttgc tgtggctgat aagacgataa gacttcaatc
     4081 ccgctacgcg ccgacgcttc ggcttcatcc agccgtgccc aggcccataa cagttgtgct
     4141 cggatgcgca ccagaaactc atgcatcggc aactgttcca gatgctgctc gttgatcagc
     4201 tggaacgctt tttcctgcgt ttcccacgcg gtttgcagga acccttgggc aaacagaatt
     4261 tcactttgct gaattaaact ccataaggcg tagtgccaga cgtcgtgctg gcgtgccatc
     4321 tgttcggttt gctgcattag cgccagtgag cgggtcaatt cgcctttgca gtgcagcact
     4381 tcacccagca ccgaggttgc cacaatgcgg ctatagaacc agcccggcgg tagctcttcc
     4441 agtgccagtt ttgccagccg ttccgcttca tccggattac catcgttaat cgccacctgg
     4501 gcgcgcagag cgttaaattc tgcgtgcatg gtgccttctc tgatgtcctt gatttcatgt
     4561 tcagcacggg ccagcagggt gttaacttcg ccgtagcgat gttggctttg catcagccac
     4621 gcctgcaata acaccaactg cggattttcc agcaaactgt cccacggcag ggccttaagc
     4681 gactcttcca gcagcgacag ttcgctatgg ttgaacaaac tccaggcgtg attaagcaga
     4741 atatcgcgca gcatcagcgc atcgcctgcc gccagcgcat gatgaatcgc ttcgctgggg
     4801 aatccctggg ccatccagct ttctgcggcg gcacggtgga tttccggcag ctccgccgcc
     4861 agttcccact ggcagcgctg gcgcaggaag ttaccaaaca gcgggtgata gcagaaccac
     4921 tcgccggtat catccatccg ctgtaaaaac agcccctgac gctcaatctc ttcgaggcgc
     4981 atttgcccgt tttcttcgcc ggtcacacgg gtgatgagtg catcgttcat tgagcgcaaa
     5041 atggcgcttt tcaacagaaa atggcgcgtt gcgagatcga cgttatccaa aacctcatcg
     5101 accagataat ccgaaagatg gctggcattg attcccgcca ggcggcgtgc cgacttatgg
     5161 gctgagtggg tattctgccg ggcggagagg gcgattagct gtagtgccgt cgcccaaccg
     5221 gaaacgtcat cgcaaatccg actgctttct gcggcttcaa tcggcgatga cagacggcaa
     5281 tcaaaaaact gcttcgcttc ctgatgggta aatgccagtt gctgactgcc aatttccagc
     5341 agttgatcac gaacacgcag attggcaatg cccagttgcg gaaggttacg tgacaacacc
     5401 accagggtga gattttctgg ttgatggcga ataaagaagc gcattgactc gtggatcact
     5461 ggattagtga tcagatgata gtcatcgatg accagataaa gtgggctatg ccattccgcc
     5521 agctcaatga aaagctgggc gaagagtgac gtcaggctgg catattgccg tttttgcgcc
     5581 atcgtctcac atatcgcaca gtgaccgttg gttgcctgtt gcacggcggc aatgagatag
     5641 ctggcgaaac gctcttgctg gttatcacct tcgtccagcg agtaccagcc gatatcgttt
     5701 ttgcctgccg cccactggga aatgagcgtg gtcttaccgt agcccgcagg acttgtgatc
     5761 agcgccagcc ggaagttgtt cgcgccggaa agtttagcca gcaggcgctc acgaaccacg
     5821 gtatggtcga gtcgaaccgg acgacttaat tttgacggaa tcagcatagt taatcacttc
     5881 actgtggaaa atgaggaaat attatttttt tgcgcttcgt aattaatggt tataaggtcg
     5941 gccagaaacc tttctaatgc aagcgatgac gtttttttat gtgtctgaat ttgcactgtg
     6001 tcacaattcc aaatctttat taacaactca cctaaaacga cgctgatcca gcgtgaatac
     6061 tggtttccct tatgttcatc agattcattt aagcaagggt ttcttcttca ttcctgatga
     6121 aagtgctatc taaaaagatg atcttaataa atctattaag aatgagatgg agcacactgg
     6181 atattttact tgtgaaactg tttcactcct ttacttaatt tatagagtta cctttcgctt
     6241 tttgaaaata cgcaacggcc attttttgca cttagataca gattttctgc gctgtatggc
     6301 attgatttgc tgctaatcct gtggtttgca ctagctttaa gtggtcgaga tcacatttcc
     6361 ttgctcatcc ccgcaactcc tccctgccta atcccccgca ggatgaggaa ggtcaacatc
     6421 gagcctggca aactagcgat aacgttgtgt tgaaaatcta agaaaagtgg aactcctatg
     6481 tcacaaccta tttttaacga taagcaattt caggaagcgc tttcacgtca gtggcagcgt
     6541 tatggcttaa attctgcggc tgaaatgact cctcgccagt ggtggctggc agtgagtgaa
     6601 gcactggccg aaatgctgcg tgctcagcca ttcgccaagc cggtggcgaa tcagcgacat
     6661 gttaactaca tctcaatgga gtttttgatt ggtcgtctga cgggcaacaa cctgttgaat
     6721 ctcggctggt atcaggatgt acaggattcg ttgaaggctt atgacatcaa tctgactgac
     6781 ctgctggaag aagagatcga ccctgcgctg ggtaacggtg gtctgggacg tctggcggcg
     6841 tgcttcctcg attcaatggc aactgtcggt cagtctgcga cgggttacgg tctgaactat
     6901 caatatggtt tgttccgcca gtcttttgtg gatggcaaac aggttgaagc gccggatgac
     6961 tggcatcgca gtaactaccc gtggttccgc cacaacgaag cactggatgt gcaggtaggg
     7021 attggcggta aagtgacgaa agacggacgc tgggagccgg agtttaccat taccggtcaa
     7081 gcgtgggatc tccccgttgt cggctatcgt aatggcgtgg cgcagccgct gcgtctgtgg
     7141 caggcgacgc acgcgcatcc gtttgatctg actaaattta acgacggtga tttcctgcgt
     7201 gccgaacagc agggcatcaa tgcggaaaaa ctgaccaaag ttctctatcc aaacgacaac
     7261 catactgccg gtaaaaagct gcgcttgatg cagcagtact tccagtgtgc ctgttcggta
     7321 gcggatattt tgcgtcgcca tcatctggcg gggcgtaaac tgcacgaact ggcggattac
     7381 gaagttattc agctgaacga tacccaccca actatcgcga ttccagaact gttgcgcgtg
     7441 ctgatcgatg agcaccagat gagctgggat gacgcctggg ccattaccag caaaactttc
     7501 gcttacacca accataccct gatgccagaa gcgctggaac gctgggatgt gaaactggtg
     7561 aaaggcttac tgccgcgcca catgcagatt attaacgaaa ttaatactcg ctttaaaacg
     7621 ctggtggaga aaacctggcc gggcgatgaa aaagtgtggg ctaaactggc ggtggtgcac
     7681 gacaaacaag tgcatatggc gaacctgtgt gtggttggcg gtttcgcggt gaacggtgtt
     7741 gcggcgctgc actcggatct ggtggtgaaa gatctgttcc cggaatatca ccagctatgg
     7801 ccgaacaaat tccataacgt caccaacggt attaccccac gtcgctggat caaacagtgc
     7861 aacccggcac tggcggctct gttggataaa tcactgaaaa aagagtgggc taacgatctc
     7921 gatcagctga tcaatctgga aaaattcgct gatgatgcga aattccgtca gcaatatcgc
     7981 gagatcaagc aggcgaataa agtccgtctg gcggagtttg tgaaagttcg taccggtatt
     8041 gagatcaatc cacaggcgat tttcgatatt cagatcaaac gtctgcatga gtacaaacgc
     8101 cagcacctga atctgctgca tattctggcg ttgtacaaag aaattcgtga aaacccacag
     8161 gctgatcgcg taccgcgcgt cttcctcttc ggcgcgaaag cggcaccggg ctactacctg
     8221 gcgaaaaata ttatctttgc gatcaacaaa gtggctgacg tgatcaacaa cgatccgctg
     8281 gttggcgata agttgaaggt ggtgttcctg ccggattatt gcgtttcggc ggcggaaaaa
     8341 ctgatcccgg cggcggatat ctccgaacaa atttcgactg caggtaaaga agcttccggt
     8401 accggcaata tgaaactggc gctcaatggt gcgcttactg tcggtacgct ggatggggcg
     8461 aacgttgaaa tcgccgagaa agtcggtgaa gaaaatatct ttatttttgg tcataccgtg
     8521 gaacaagtga aggcaattct ggccaaaggc tacgacccgg tgaaatggcg gaagaaagat
     8581 aaggtgctgg acgcagtatt gaaagagctg gaaagcggta aatacagcga cggcgataag
     8641 catgccttcg accagatgct gcacagtatc ggcaaacagg gcggcgatcc gtatctggtg
     8701 atggcggatt tcgcagccta tgtagaggca caaaagcagg tggatgtgct gtaccgcgac
     8761 caggaggcct ggactcgcgc ggcgatcctc aataccgccc gctgcggtat gtttagctcg
     8821 gatcgctcta ttcgcgatta tcaggctcgt atctggcagg caaaacgcta aggaagctcg
     8881 atggaaagca aacgtctgga taatgccgcg ctggcggcgg ggattagccc tcattacatc
     8941 aatgcccacg gtaaaccgca gtcgattagc gccgaaacca aacggcgttt gcttgacgcg
     9001 atgcatcaac gtaccgccac gaaagtggcg gtaacgccag tcccgaatgt catggtttat
     9061 accagcggca aaaaaatgcc gatggtggtg gagggcagcg gcgaatatag ctggctgctg
     9121 accaccgaag aaggaacgca gtacaaaggc catataacgg ggggcaaagc gttcaatcta
     9181 ccgacgaagc tgccggaagg ttatcacacg ctgacgctta cccaggaaga ccagcgcgcg
     9241 cattgcaggg tgattgtcgc cccgaaacgc tgttacgaac cgcaggcgtt gctgaataaa
     9301 caaaagctgt ggggtgcctg cgtccagctt tatacgctgc gatcggaaaa aaactggggt
     9361 attggggatt ttggcgatct caaagcgatg ctagtggatg tggcaaaacg tggcggatcg
     9421 ttcattggtc tgaacccgat tcatgcgctc tatccggcaa atccggagag cgccagcccg
     9481 tatagcccgt cttctcgccg ctggctgaat gtgatttata tcgacgttaa cgccgtcgaa
     9541 gatttccatc ttagcgaaga ggctcaggcc tggtggcagt tgccgaccac gcaacagacg
     9601 ctgcaacagg cgcgggatgc cgactgggtc gattactcca cggttaccgc cctaaaaatg
     9661 acagcattac gaatggcgtg gaaaggtttc gcgcaacgtg atgatgagca gatggccgcg
     9721 tttcgccagt ttgttgcaga tcaaggcgac agcctgttct ggcaggcagc ctttgatgcg
     9781 ctacatgccc agcaagtgaa agaggacgaa atgcgctggg gctggcctgc atggccagag
     9841 atgtatcaga acgtggattc accagaagtg cgtcagttct gcgaagaaca tcgcgatgac
     9901 gttgattttt atctctggtt gcagtggcag gcttacagcc agtttgccgc ctgctgggag
     9961 ataagccagg gctatgaaat gccgattggc ttgtatcgtg atctggcggt tggcgtagcg
    10021 gaaggtgggg cggaaacctg gtgcgaccgt gaactgtatt gcctgaaagc gtcggttggc
    10081 gcgccgccgg atatcctcgg cccgttgggg cagaactggg gattgccgcc aatggacccg
    10141 catatcatca ccgcgcgtgc ttatgaaccg tttatcgagc tgttgcgtgc caatatgcaa
    10201 aactgcggcg cattacgaat tgaccatgtg atgtcgatgc tgcgtttgtg gtggataccg
    10261 tatggcgaga cggcagatca gggcgcgtat gttcactatc cggtggatga tctgctctcg
    10321 attctggcac tcgaaagtaa acgtcatcgc tgtatggtga ttggtgaaga tctcggtacc
    10381 gtaccggtag agattgtcgg taagctgcgc agcagcggtg tgtactccta caaagtgctc
    10441 tatttcgaaa acgaccacga gaagacgttc cgtgcaccga aagcgtatcc ggagcagtcg
    10501 atggcggttg cggcgacaca tgacctgcct acgctacgcg gctactggga gagcggggat
    10561 ctaacgctgg gcaaaaccct ggggctgtat ccggatgaag tggtactgcg cggtctgtat
    10621 caggatcgcg aactggcgaa gcaagggctg ctggatgcac tgcataaata tggttgtctg
    10681 ccaaaacgtg ccgggcataa ggcatcgttg atgtcgatga cgccgacgct taatcgtggt
    10741 ttgcagcgct acattgccga cagtaacagt gctctgttag gactacagcc ggaagactgg
    10801 ctggatatgg ccgaaccggt gaatattcct ggcaccagtt accagtataa aaactggcga
    10861 cgcaagcttt ccgcaacgct tgagacgatg tttgccgatg atggcgtgaa caagttgctg
    10921 aaggatttgg acagacggcg cagagctgca gcgaagaaga agtagagtgc ggttgatgcc
    10981 ggatgcgatg catccggcag tcagtgttat caaatcacca tattcagcag cagacagcct
    11041 accagaccgc acaccgagat aatggtttcc agcatcgacc aggatttgat ggtctcgccg
    11101 atagtcaggt taaagtactc tttgaacagc cagaagcccg gatcgttcac atgagagaaa
    11161 atcacactac cggaacccac cgcaataacc atcagctccg ggctaacacc cgtcgttgca
    11221 atcagcggtg ccgcgatacc acccgcagtg attgccgcaa cggttgcgga acccagcgcg
    11281 atacgcagta cggcagcaat cgaccaggcc atcagcagcg gagaaatgtt ggtttcgtgc
    11341 atcatggaag caatgtattt gtccacgccg ctgtctacca gcacctgctt gaacgcaccg
    11401 ccaccaccga tgatcaacag catcatcgca atgattttga tggaagaaac cagcgtgtcg
    11461 ttaatctgat ccattgaacg accacggttc agaccaaagg tgaacatcgc aatcagcacg
    11521 gcaatcagcg tagccattac cgggtcaccg aggaactccg ctaccggcag gaaagcgtga
    11581 cctttcggca ggatcatttc ggcaatcgca cgcatcgcca tcagtactac cggcaccaga
    11641 gaggtccaga cgctgacgcc aaagctcggc atctcttctt cgctgaaggt tttcgcgctg
    11701 tagagacctt ccggaattgg cttatcgata cctttcaaca cgcgagcgta aaccggaccg
    11761 gcgagaatca cggtcgggat tgccagaata gtaccgtaca gcagggtttt acccatatcg
    11821 gcattgaaaa tggtggcaat cgcagtcgga cccggatgcg gtggcaggaa gccgtgggtc
    11881 acagacagtg cagctgccat tggtacacca acatacagca gtggaatatt cgcagaagcc
    11941 gcgatggtaa acaccagcgg cagcatcagc acaaagccca cttcatagaa cagggcaaaa
    12001 ccaacggtaa aaccggtcag taccaccgcc cactggatgt gttttttacc aaatttggca
    12061 atcagcgtgg tggcgatacg ttgtgcgcca ccgcagtctg ccagcatttt gcccagcatt
    12121 gcgccaaaac ccatgatcag ggcaaggcta ccgagcgtcc cgccgacacc ggctttgatg
    12181 gagccaataa ctttatccag cggcatccct tgcattaatc caaccgcaag cgccacgagg
    12241 acgagagcga tgaagccgtt cattttgaag cggatcatca ggagtaataa caagattaca
    12301 ccgatagcaa caatgactaa tggcatgatt tacctggcct ttcatttgtt atgggtaacg
    12361 tcaattttct gacgacaagc tctaattatc ccaatcggga acagagatat tgcggcacca
    12421 cgactgatac ccactaaaac taattattgt agtcagatgt caggagtatg tttggtaccc
    12481 acgtgaatga tacgggtaac atctggcgtt tgagaatcac cagagcgggg taaatttaaa
    12541 ttatgagagg ttggtcatat tatcgcgggg aaacgaaggg agggatttgc caaagcaatg
    12601 ctgcgccaac gtctggcaca tgttcaacgt aggcccgaaa tgacgcttta gcgtcgcatc
    12661 gggcaatcta caaaagaggg gataacttag tagtaggagt gttcgccgcg ctggtgttcg
    12721 gtgagatcgc gcacaccttt cagctccggg aattcgttta gtagctgctt ctcgatccct
    12781 tctttcagcg tcacatcgac catggaacaa ccgttacagc cgccgccaaa ttgcagaatg
    12841 gcgtaaccgt cttcggtgat ttccatcagt gaaacgcggc caccgtgacc agcaagctgt
    12901 gggttgatct gcgactgcag catatactcc acgcgctcca tcagtggtgc atcgtctgcc
    12961 actttacgca ttttggcgtt aggcgctttc agtgttagct gagaacccaa ctggtcggta
    13021 acaaaatcga tctctgcatc ttccaggtat ggtgcgctta actcatcaac atacgcggtc
    13081 agcaggtcaa atttcagggc ggtgtcggtg gcttccacgg catccggcgg acaataagaa
    13141 acaccacatt cagcgttagg cgtgccaggg ttaatcacaa atacgcggat ttgtgtccct
    13201 tcttcctgat ttgccagcag tttggcaaag tgcgcttgtg cagcatcgga aatacggatc
    13261 atagtaatgg cctaatagtt gactatttta gttggttata atacgcccat catcgaggct
    13321 ctacaaggtt cgacaaaggc accagacctg gacagtcgcc gcaccattgc gtaaaagcaa
    13381 ctgcgcaatc tctgcgacgg tacttccggt ggtaacgaca tcatccacaa tcaccatatg
    13441 gcgaccttgc acaggcaatt caaggcgaaa ggcatttttc aggttgcgcc tgcgtagccg
    13501 ggcgctaaga aaatgctggg tcgcagtggc ccgtgtacgt gtgatggctt cgctatccca
    13561 ttggcagtgc aaccagcgtg ataacggctg acacagcaaa tcgctctgat taaatccccg
    13621 acgccagtga cgccgctgcc ataacggaac gctgatgata cgatccggca attgcaaccc
    13681 ggtggtgcga cgagcgtgta agacttccaa tagtaacaga cgtgacaggg cgctggcgat
    13741 ttcgctgcgc cgggaaaact taagctggtg gatgagcaga cttaacggcg gcgcatagtc
    13801 ggcaaccgtg accagtcttt gccagggcgg cggtttttgc aggcagcgac cgcagggaag
    13861 atgggagtgt gtggcgggta atccacattg tgggcataac gttttatctg tacgggcggc
    13921 gcgtgagcag accgaacaaa tcccccaatg acctaatgcc agtggcattc ggcatagcca
    13981 gcataatccc ggtactgtta gcatatgttc atccttgtaa gtcaaaagag aacaatagcg
    14041 gatgaataac atctggtggc agaccaaagg tcaggggaat gttcatcttg tgctgctgca
    14101 cggatgggga ctgaatgctg aagtgtggcg ttgcattgac gaggaactta gctcgcattt
    14161 tacgctgcat cttgttgacc tgcccggctt cgggcgtagc cggggatttg gtgcgctgtc
    14221 acttgcggag atggccgaag ccgtgctgcg acaggcacct gataaagcca tttggttagg
    14281 ctggagtctg ggcgggctgg tggcaagcca gattgcgtta acccatcccg agcgtgttca
    14341 ggcgctggtc accgtggcgt cgtcaccttg ttttagtgct cgtgacgagt ggccggggat
    14401 aaaaccggac gtgctggcgg gatttcagca gcaactcagt gatgattttc agcgtacggt
    14461 ggaacggttc cttgcgttac aaaccatggg tactgaaacg gcgcgtcagg atgcgcgggc
    14521 gttgaagaaa accgttctgg cgttaccgat gccggaggtt gacgtgctca atggtgggct
    14581 ggaaatcctc aaaacggttg atctccgctt gccgctgcaa aacgtaccca tgccgttttt
    14641 gcgactgtat ggctatctcg atggtctggt gccgcgcaaa gtggtgccga tgctggataa
    14701 actctggcca cacagcgagt catacatctt cgccaaagcg gctcatgcgc cgtttatttc
    14761 gcatccggtc gagtttcgtc acgtgctggt ggcgttgaag cagagggtgt aggcactttt
    14821 gaatggcgag acagaacttt actgactcgc cgcagccaac tcttcttctg acagcccggt
    14881 aaagcgcatg atgtctgcaa gagggactcc ggattcaagc attattttgg ctatatgcag
    14941 ggctttggat tgttccccct cctgccgcaa tctttccgca atagtcatta aactctcctt
    15001 gtgtttcggt gatcgttcgg caacgccgtc gataaaatcg ttaaaacgta ccgcatcgcc
    15061 ggtttgcagt atgtaattaa acagcccttt gatttgtctg tcattagcgt atccactact
    15121 taataagcag gccatttgct ctaccagccc catcaggtcg cgttgacgaa tatgtttttg
    15181 aattaactcc agcagcgtca tgcgtcggtg ctgcatgatt tcatcatcag gcatgacggt
    15241 gatatcaatc agcggaaatg cggaggcata aagctgcctt gccagtttgg gatcggcgaa
    15301 acaatccagc caacacagcg aatagggata ggggctttca ataccgtggt aaaacaacaa
    15361 tggcaccacc atcggcaacg ttttgtatcc ggcatcaagg tgattttgca ttgcggcaat
    15421 agcgtaacgc atcatgcgaa atgcgatcag tttgtttgag gtgctttgat gttcaatcag
    15481 acaatagatg tatcctggtc cttgttctgt tttcaccgac cacagcacat cggaatagct
    15541 ttcacgcaga tcgtcatcaa taaagctgct cgactccagc ttgagggttt tcatatcaca
    15601 gagcgcgtga atgggtgctg gtaaatgaaa cgcaagaaaa tcgcgtgccg tctccggttg
    15661 gcgtaaaaag agtttgaaca gcgcatcgtg tggggtggaa ctctgctttt tgctcatggc
    15721 gttccttcac ctgttgtttg aatgccgcga ctctacgcgc cactccacac ctgtgcgtta
    15781 gccagttttc atccctgcga gacgctttca gaggaactta cgtcgcgctg caagaattgc
    15841 atttttatgg aaagccgcac gcaaaaaagc ccggatgcaa acgcataccg ggcttttcaa
    15901 aacaggcgat ggagtatggt taacgcagcg cccaccactc gcgcagacag gcttttcctt
    15961 ccgggcagct tttacaactg ccggagaggc agccgtcagg ttcttcctga atccgcacgg
    16021 ctttgcccat actttccagt tgttgcagca tggcgttaat cattggctgt ggagtgttca
    16081 atgtctggct tatctgggcc gcttccatac ggccccgtaa cgccagcaaa tcacgcacct
    16141 gaataagtga agccattaat ggcaatcacc ggtggtgctg gctgcgcagc aactacttac
    16201 cgacttgcgg gttgccagca gttcgatatc cacccggcta cgcgcgcggc gcagcagacc
    16261 gataaccacg atgttaaaca ggataaccgc cagaatgcac accaggctgt aagttgggtg
    16321 ctgactgtag ctggcgactt gatagaacaa tgttgccagt gagtaagcga tattcagccc
    16381 ccacaggatg gagaagccca tccagccacg gcttgattca cgggcgatgg ctcccatcac
    16441 cgagatacat ggtacataca gcaggacgaa aatcaggtag ctgtatgcgg cagctgcgct
    16501 accgaatttc tgatccatca cgcccatcgc cccggtaccc atttcgccgt cgcctttgct
    16561 agcttcaatg gggttcatca gtacgctaag gctgaaggtg tctttcaggc tctgccaggt
    16621 ttcatctacc gcgctgaaca actcttcacc gaggttaaat tccgccggat tgaactcttc
    16681 gtcctgaata ttttctgcgg tgtagagggt gttgagcgta cccaccacca cttctttcgc
    16741 cattgcaccc gtaaacaggc caactgttgc ctgccagtta tcttcatgca cgccaatcgg
    16801 cttgaagacc ggggtgatca cccggctgac ggaagccagc gccgagtcgt tgatgttatc
    16861 gacgattttc ccgctcaacg agaagctgtt gaaagcgctc aggaaaatgc tgacgatgat
    16921 aatcacttta ccagcacgca gaacgaagcc tttcagacgc tgccaggtct ggataatcag
    16981 gcttttaacg tgtggtacat ggtagaccgg cagctccatg acaaacggcg tcgcttcacc
    17041 gcgcatgatg gtgtacttga gcatcaggcc agtcagcacc gccatcacga taccgagcat
    17101 atacagcgag aagaccgcca gcgcaccgtt ctgcccgaag aaggcagccg cgaatactgc
    17161 gaagatagcc agacgcgcgc cgcaggacat aaacggtgcc atcatgatgg tcatcagacg
    17221 ttcacgcggt gcatcaagcg tacgtgcacc cattaccgac ggtacgttac aaccgaaacc
    17281 gacgatcagc ggcacaaagg atttccccgg caagcccagc gcctgcatca ggcggtccat
    17341 cacaaacgcc gcacgcgcca tatagccgga gtcctcaagg aaggagagga acaggtacat
    17401 catgccgatc tgcggcacca gtggcagcac ggtgttaata ccgccaccca ggccctgggc
    17461 gaggaagata gtcagccagt ccgggaagtg gagcgtgtag ccaatccatt gaataccatg
    17521 cacaaataga gccacggagc cgacgtcaaa cagcggctgt aacgccccgc cgatgttgat
    17581 agccagcagg aacatcaggt acatcacaaa gaggaaaatc ggcagaccga ggaaacggtt
    17641 gagcacgatt ttatctaccg cagtggtgaa acggctgggt tctgccgtca gggtgttgct
    17701 taccacatca cagatggcag caatgcactg gtaacgcgca tcggcaatgt gcagcgccgg
    17761 atcgtccatc tcattacgca gacgggcgag ggcggcatca agatgctgcg acgcttcacc
    17821 ggcgtaggcg cggctgtaga tatcgccttc cagcatttgc aggcccagcc agcgacgttg
    17881 tttcagcggg atgtcggaag gcatcacttt tgccagtgaa tctgcttcgt tgagtagcgg
    17941 ctgtgcgtaa tgcaccagtt ccacattctc gttagcttta tagcgatcaa tcgccagctt
    18001 gagcgcttca ataccgcgac cacgggttga aaccagcggg atcactgggc agccaagacg
    18061 cgccgacaga gcatcaattt caatacgaat attttgcttc tcggcaatgt cgagcatgtt
    18121 cagtgccacg atgcagggaa tgccgagttc cagcagttgt agcgtcaggt acaggttacg
    18181 ctcaaggtta gacgcatcca ccacgttaat cagcaggtcg gcgtcgccac tcaaaatgta
    18241 gtgacaggcg atttgctcat cgagcgaggt ctgcgatgag atggtggtca gagaataggt
    18301 gccgggcagg tccaccagcg tgacctgatg atcggtggtg gagaattgcc cttctttacg
    18361 ttcgacggta acgccagccc agttacctac acgctgacgt gcgccagtga gctggttaaa
    18421 taaggttgtc ttgccagaat ttggattacc aattaagcct atggttaatt ttttcattgt
    18481 tgttatcacc gtattaacag gaaaccgctt ccacttctaa taaggccaga tcttttttgc
    18541 gtaataccag gctcacacga cgggtttcga tatgaatggg gtcgccgagt ggagcgacgc
    18601 gcaccacatt aaaagaggag ccaggtaaca tgccaagaga aagcagtttt tggcgatatg
    18661 ccgggctgat ttcacgggaa aagccagtga ttttccacgc agtatctgga gtgtattgca
    18721 taggtgccta cttgtttctc attaactgga taactacctc atggggcgtt tgacagcgac
    18781 atgagaccag atgatttgcc aacgaaggag agattaaata atcaatagta caatgataat
    18841 gagaatggtt tctatcagca atacttaaaa tgtgtgcgaa tgttggtttg aataataatc
    18901 tgactcaatg ttgatgtggc tcaaggttat atcgcagcga aaatataatt cgaatagcga
    18961 attaccagga ctggctaatg tttaattaag gtttcagttg ttaatgaagt gataaatatt
    19021 aacgaaatat attgtgagtt tggctgtgga ataaatatgc cggagatatt ctccggcact
    19081 tttatgtcag gcaaggcgtt cgttgccaga tgcggcgtga acgccttatc gcgcaggttt
    19141 aggggccagt ccgggcgttt aacgtttttt gcccattgct gccgccagcg cgtccatcat
    19201 cgcgctgtta ccggcaggct gcgcttcacg accgcgtggt ttggctgccg ggcggttgtt
    19261 ttgcgggcga tcgttaccgc cgccgcgacg agagttggtt tcgccaggct gctcgtccag
    19321 acgcatagtc agggcgatac gtttacgctg aagatccact tccagcactt tcaccttcac
    19381 aatgtcgccc gctttcacca cggtatgcgg atcttccaca aacttgttcg acaatgaaga
    19441 gatgtgaacc aggccgtcct gatgcacgcc gatatcgaca aacgcgccaa agttggtgac
    19501 gttggtgaca gcgccttcga ggatcatacc cggttgcagg tcgttcattg tctcgacgcc
    19561 atcggcaaac tgagcggttt taaattccgg acgcggatcg cgacccggtt tttccagctc
    19621 tttgatgatg tcagttactg tcggcacacc gaatttttca tcagtaaagt cagacgcttt
    19681 caggttacgc agttcgctgc tgttacccat cagatctttc agtgcctgct gtgttgctgc
    19741 cagaatgcgt tccaccaccg gataggcttc cgggtgaacg gtagaggcgt ccagcgggtt
    19801 gtcaccgtgg ttaatgcgca ggaagcccgc gcactgctcg aaggctttcg gccccagacg
    19861 gctgactttc agcagttgct gacggttctg gaactggccg ttctcatcgc gccaggccac
    19921 gatgttttgc gccatcattc gcgtcaggcc cgccacgcga gttaatagcg gaacagaagc
    19981 ggtgttgaga tcgacgccaa cggcgtttac gcagtcttct actactgcat ccagtttgcg
    20041 ggccagttgc gtctggctga cgtcatgctg atactgacct acgccgatag atttcggatc
    20101 gattttcacc agctccgcca gcggatcctg caaacggcgg gcgatagaaa ccgcgccacg
    20161 cagcgaaacg tcgagatccg ggaactcctg cgctgccagc tcggaagccg agtaaaccga
    20221 cgcgccagct tcgctgacga tcactttctg tgcggtcact ttcgggaact gcttctgcac
    20281 gtcgagatag aaacgttcgg tttcgcggga agctgtaccg ttgccgatcg ccaccagttc
    20341 aacgttatgc ttttcacaca gcgcagcaac agtcatcgct gcttttgcgg cttgtccggt
    20401 gtgcgggtaa atggtatcgg tcgctaccag tttgccagtg gcatcgacca ccgccacttt
    20461 taccccggta cgcagacccg gatcgaggcc catcgttgca cgcagtccgg caggggcagc
    20521 catcagcaga tcgtgcaggt tacgggcaaa gacgttgatt gcttcatttt ccgcacgttc
    20581 gcgcacggtg cccatcagtt cggtttccag atgcatcagc accttgatgc gccacgtcca
    20641 gctcaccacg cctttgcgcc agctatccgc cggggcattg ttcaggcgca ggccaaggtg
    20701 atccatgatg atttgctcgc aatagctctc tttgggcggc tcatcgaact gtggatcggc
    20761 attcagcgaa agctgaagta cgccttcgtt acgcccacgg aacatcgcca gcgcgcggtg
    20821 agaaggcacc gtggacaacg gttcgtgatg atcgaaatag tcgcggaatt tcgccccttc
    20881 ctcttcttta ccgctcacca ccgtagaaac caaatgcgcg ttcttccaca gataatcacg
    20941 cactttcgcc agcagcgcgg catcttcggc aaaccgttcc atcaggatat agcgcgcgcc
    21001 gtccagcgcg gctttggtat ctgccacgcc tttatcggca tcaatatatt gcgcagcggc
    21061 gacttctggc gtgtgtgacg gatcgctcca cagcaggtca gccaacggct caagccctgc
    21121 ttcaatggcg atttgcccgc gggtgcggcg tttaggtttg tagggcaggt agaggtcttc
    21181 gagttcggtt ttgcttaggg tggcgttgat ggcattcgcc agatcatcgg tgagtttgcc
    21241 ttgctcggaa atggacttga ggatcgcctg acgtctctct tccagctcgc gcaggtatcc
    21301 cagacgcgtt tccagattac gcagctgcgt gtcatccaga ccgccggtga tttccttacg
    21361 ataacgtgca ataaaaggca cggtattccc ttcgtcaagc aggcgaacgg cagcgtcaac
    21421 ctgttccggg cgcgcctgaa tttcacccgc aataatgcgg cagaacgaat cattcatcat
    21481 ggtttgagtt catcttttcg gatcaaaaat caggggatag ttatacggac tggctggcaa
    21541 aaatgccagc catcggcagg aggttaagac tcttccttac ggtttcacgt actcgatagc
    21601 attaacatac cagctcgctt ccccggcagg agtattcacc acagccagat cgccgacttc
    21661 ttttttcagc aatgcgcggg ccatcgggga atcgatagag atgtaatctt tacggccaaa
    21721 aatttcatcg tagccgacaa tacggaaacg gtgagtcacg ccatcgtcgt tttcaatctc
    21781 cacccacgcg ccaaaaaaga ctttgccttc ctgctgcggg gagtaatcga cgattttgag
    21841 attttccatg catttagtga gatagcgcac gcgacggtcg atttcacgca ggcgcttttt
    21901 attatactga tagtcagcat tttcgctgcg gtcgcccaga cttgcggccc aggtcacctt
    21961 ttttgtgacc tccgggcgtt cttcacgcca gagataatta agctcttgtt tgagtttttc
    22021 atacccttcc cgggtaacca ggggcgtttt catctcgttg attccctttg tctgtttgat
    22081 aatgcgcaca ttgggtataa cgtgatcata tcaacagaat caataatgtt tcgccgaata
    22141 aattgtatac ttaagctgct gtttaatatg ctttgtaaca atttaggctg aaattcatac
    22201 cagatttagc tggtgacgaa cgtgagcttt tttaagaata cacgcttaca aattgttgcg
    22261 aacctttggg agtacaaaca atgcaagaga actacaagat tctggtggtc gatgacgaca
    22321 tgcgcctgcg tgcgctgctg gaacgttatc tcaccgaaca aggcttccag gttcgaagcg
    22381 tcgctaatgc agaacagatg gatcgcctgc tgactcgtga atctttccat cttatggtac
    22441 tggatttaat gttacctggt gaagatggct tgtcgatttg ccgacgtctt cgtagtcaga
    22501 gcaacccgat gccgatcatt atggtgacgg cgaaagggga agaagtggac cgtatcgttg
    22561 gcctggagat tggcgctgac gattacattc caaaaccgtt taacccgcgt gaactgctgg
    22621 cccgtatccg tgcggtgctg cgtcgtcagg cgaacgagct accaggcgca ccgtcacagg
    22681 aagaggcggt aattgctttc ggtaagttca aacttaacct cggtacgcgc gaaatgttcc
    22741 gcgaagacga gccgatgccg ctcaccagcg gtgagtttgc ggtactgaag gcactggtca
    22801 gccatccgcg tgagccgctc tcccgcgata agctgatgaa ccttgcccgt ggtcgtgaat
    22861 attccgcaat ggaacgctcc atcgacgtgc agatttctcg tctgcgccgc atggtggaag
    22921 aagatccagc gcatccgcgt tacattcaga ccgtttgggg cctgggctac gtctttgtac
    22981 cggacggctc taaagcatga ggcgattgcg cttctcgcca cgaagttcat ttgcccgtac
    23041 gttattgctc atcgtcacct tgctgttcgc cagcctggtg acgacttatc tggtggtgct
    23101 gaacttcgcg attttgccga gcctccagca gtttaataaa gtcctcgcgt acgaagtgcg
    23161 tatgttgatg accgacaaac tgcaactgga ggacggcacg cagttggttg tgcctcccgc
    23221 tttccgtcgg gagatctacc gtgagctggg gatctctctc tactccaacg aggctgccga
    23281 agaggcaggt ctgcgttggg cgcaacacta tgaattctta agccatcaga tggcacagca
    23341 actgggcggc ccgacggaag tgcgcgttga ggtcaacaaa agttcgcctg tcgtctggct
    23401 gaaaacctgg ctgtcgccca atatctgggt acgcgtgccg ctgaccgaaa ttcatcaggg
    23461 cgatttctct ccgctgttcc gctatacgtt ggcgattatg ctattggcga taggcggggc
    23521 gtggctgttt attcgtatcc agaatcgacc gttggtcgat ctcgaacacg cagccttgca
    23581 ggttggtaaa gggattattc cgccgccgct gcgtgagtat ggcgcgtccg aggtgcgttc
    23641 cgttacccgc gcctttaacc atatggcggc tggtgttaag caactggcgg atgaccgcac
    23701 gctgctgatg gcgggggtaa gtcacgactt gcgtacaccg ctgacgcgta ttcgcctggc
    23761 gaccgagatg atgagcgagc aggatggcta cctggcagaa tcgatcaata aagatatcga
    23821 ggagtgcaat gccatcattg agcagtttat cgactacctg cgcaccggac aggagatgcc
    23881 gatggaaatg gcggatctca acgcagtact cggtgaggtt attgctgccg aaagtggcta
    23941 tgagcgggaa attgaaaccg cgctttaccc cggcagcatt gaagtgaaaa tgcacccgct
    24001 gtcgatcaaa cgcgcggtgg cgaatatggt ggtcaacgcc gcccgttacg gcaatggctg
    24061 gatcaaagtc agcagcggga cggagccgaa tcgcgcctgg ttccaggtgg aagatgacgg
    24121 tccgggaatt gcgccggaac aacgtaagca ccttttccag ccgtttgttc gcggcgacag
    24181 tgcacgcacc attagcggca cgggattagg gctggcgatt gtgcagcgta tcgtggataa
    24241 ccataacggg atgctggagc ttggcaccag cgagcggggc gggctttcca ttcgcgcctg
    24301 gctgcctgtg ccggtaacgc gggcgcagag cacgacaaaa gaagggtaaa taaacgggag
    24361 gcgaaggtgc ctcccgtttt gctttctata agatactgga tagatattct ccagcttcaa
    24421 atcattacag tttcggacca gccgctacca gcgcggcacc cgcaggggtg tcggtgtatt
    24481 tatcaaagtt gtcgataaac agtttcgcca gggtttcggc tttttcctgc cactgttccg
    24541 gggaagcgta ggtgttacgc ggatcgagaa tcttcgtgtc tacgcccggc agttcggttg
    24601 ggatcgccag gttaaacatc ggcagagtga aggtttctgc attatccagc gaaccgttga
    24661 ggatggcgtc gataatggcg cgggtatctt taatcgagat acgtttgcca gtgccgttcc
    24721 agccagtgtt aaccagataa gcctgcgcgc ccgccgcctg catacgtttc accagcactt
    24781 ctgcgtactg agtcgggtgc agcgacagga atgccgcgcc gaagcaagcg gagaaggttg
    24841 gcgtcggttc ggtgatgcca cgctcagtac cggccagttt ggcggtgaag ccagagagga
    24901 agtggtactg ggtttgatcg gcagtcagac gagaaaccgg tggcagcaca ccaaaggcgt
    24961 ctgcggtcag gaagataact ttcgttgcgt gacccgcttt tgaaaccggc ttaacgatgt
    25021 tatcgatgtg atagatcgga taagaaacgc gggtgttctc ggtttttgaa ccatcatcaa
    25081 agtcgatagt gccatcttca cgcacggtga cgttttccag cagcgcatcg cgacggatag
    25141 cgttgtagat ttcaggttcc gcttctttcg acagcttgat agtttttgcg tagcagccgc
    25201 cttcgaagtt aaacacgccg tcatcgtccc agccgtgttc gtcatcgcca atcaggcgac
    25261 gtttcgggtc ggtggaaagg gtggttttac cggtgccgga aaggccgaag aacaccgcaa
    25321 catcgccttt ctcgctaacg ttggcggagc agtgcataga agcgatacct ttcagcggca
    25381 gcaggtagtt catcatcgag aacatccctt tcttcatttc gccgccatac caggtgccgc
    25441 caatcagctg catgcgctcg gtcaggttaa aagccacgaa gttttcggaa ttcagaccct
    25501 gttctttcca ctgcgggtta gtgcacttcg cgccgttcat aacgataaag tctggtttga
    25561 aacctgccaa ctcttcatcg ctcgggcgaa taaacatgtt tttgacaaaa tgcgcctgcc
    25621 aggccacttc ggtgatgaaa cggacggaaa gacgagtatc cgggttcgca ccacagaagg
    25681 cgtcgacaac gaacagacgt ttgccggaaa gctgtttggt caccaggcct ttcagatgtt
    25741 gccaggtttc cggagagaga ggtttgttat cgttcttacc tttgcctttg tctgcccacc
    25801 agaaagtatc gcgagtggta tcgtcacgga cgatatactt atcttttggt gaacgaccgg
    25861 tgaagatccc ggtatcgacg gcaacggcac ccagattagt taacaccccg cgctcataac
    25921 ctgtcaggct cggatcgagc tcttcctgat acagcaggtc gtagcttggg ttgtaaacga
    25981 tatcatgtac gtcactgata ccataagcct cgagttcttg cggggtcaaa ccattgttaa
    26041 cgcgcatttc actgctcctt agccaatatg tattgcctga atagtaaagt ctttttgggg
    26101 gtgttaaccg cgacaaggct catagattta cgtatctgga gaaattcata tttgacaaaa
    26161 aactccgtga ttcctgtcac gaaacggttg ctattatcgc agaaatagca ttttaggtgt
    26221 ggaaaatgtt ctcaaaaggt taaatcttgg tttatatggc gggaatattg tgagtggaat
    26281 cgcattcctg aatgaaattg atgcaaattc aaccggtaaa gtatcgattc accacgctaa
    26341 ctttttttga ttattttctg aaaaaagcaa aatactactg cccggatgcg ataattaacc
    26401 caccttttga ttctggataa ccaacatgga taacgttgaa ctttcacccg caacacgctg
    26461 ggggatgatt gctaccggat tacttcaggg tctggtttgc tacctgctga tcgcctggct
    26521 ttccggaaaa aatcacagtt ggattgttta tggcgtgcct gcgaccgtcg cgttttcatc
    26581 cgttttactc ttctccgtga tctcttttaa acagaagcgc ctttggggat ggctggcgct
    26641 ggtgtttatt gccacgctgg ggatgagcgg ctggctgaag tggcaaactg atggcatgac
    26701 cccctggaga gctgaaaagg cgctttggga ttttggttgc tatctgctgc tgatggcaat
    26761 gttgttgcta ccgtggatac aacaaagcct tcgcatccgc aatgacagta gccgctatcg
    26821 ctatttttac cagtcagtat ggcataacgt acttatatta ctggtgattt ttctcgccaa
    26881 tggtctgacg tggctggtgc ttttactgtg gagtgagttg tttaaacttg ttggcatcac
    26941 gtttttcaaa acgctctttt ttgcaaccga ctggtttatt tatctcacgt taggtctggt
    27001 taccgcgctg gcggtgatcc tcgcgcgaac acagtcacgt ttaatcgact ctattcaaaa
    27061 gttgttcacg ttaatcgcca cggggttgct gccgttagta tcattgctaa ccctgatgtt
    27121 tatcatcacc ctgccgttta caggcctgag tgcgatttct cgccacatct ccgccgccgg
    27181 gttgctgtta acgctggcct ttttgcaatt gatcttaatg gctattgtcc gcgatccgca
    27241 aaaagcgtca cttccctgga cggggccgtt gcgttgcctg attaaaaccg ctctgctggt
    27301 tgctccgctg tatgtgttca tcgccgcctg ggcgttatgg ctgcgggtcg ctcagtacgg
    27361 ctggactgtc gaccgcttgc agggcgcgct ggcggtgctg gtgttactgg tatggtcgct
    27421 ggggtatttt gtcagtatcg tctggcgtaa agggcaaaat ccccttgttc ttcagggcaa
    27481 agtgaacctt gcggtttcgt tattggtgtt ggtaatactg gtgcttctta attcgccggt
    27541 gctggacagt atgcgcatta gtgtgaacag tcatatggcg cgttatcaga gcggcaaaaa
    27601 cacgccagac caggtaacta tctacatgct cgaacacagc ggtcgctatg gacgtgcggc
    27661 gcttgagtcg ctgaaaagcg atgccgagta tatgaaagac ccgaaacgcg cgcgggatct
    27721 actgatggcg ttagatggag agcaacatct tcagcaacag atatcggaaa aagtattagc
    27781 cgaaaatgtg ttaattgccc ctggttctgg taaaccggat gcgacattct ggtcggcctt
    27841 aatccaggat cgctataacg tgatgacctg tattgaaaaa gacgcctgcg tactggtcga
    27901 gcaagatctg aatagtgatg gcagggcaga gcggatcctc tttgcctttg atgatgaaag
    27961 atacattgtc tatggctttg atcctgacaa gaaagaatgg caagagctta cgatgagttt
    28021 acttccgcgt gatataacga aagaaaaatt actcacagct gcgaaggatg gaaaactggg
    28081 aacgaagcct aaagcgtggc gcgatcttgt agtggatggt gaaaggctgg atgtgaatct
    28141 gaatgagtga caaagcgcat caggtgtatt agtcgtaaaa tccggcagag ctctctctgc
    28201 cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc ggatttcagc
    28261 aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca tgtcaatttc
    28321 gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca gcgcatcggc
    28381 gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt aaaccgtcac
    28441 ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca gcagttcttc
    28501 ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct gggcattttg
    28561 cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta cgtcgccggt
    28621 gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt ccaggcaggc
    28681 cgccagggta tcaccttcca gacccaccac gccctgatag cgttcacctt cgctcggggt
    28741 aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat tttctggaat
    28801 ttcgccctgc acgcgcgcca caccgcgcat ctgctggttg ttgttaccgt taataaccgc
    28861 cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa acttcagcgt
    28921 agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa cgggctgcgg
    28981 ataatcgtga ttctcaagga gctgttgcag ggtttccgaa acggttacca gttcgccgcg
    29041 cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca taatcatctc
    29101 tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt cgcggcgctc
    29161 ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt tacgtgccag
    29221 cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca aggctgcctc
    29281 gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt cgtcatttcc
    29341 ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc tgcgctgccc
    29401 gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg ttttataaaa
    29461 acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt ctttcatggc
    29521 gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac ggatggcgtt
    29581 gatactgttt ctcggcaatc cctgagtcag gattagtcac gccgaggcag taacgaatac
    29641 caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc gttctttcag
    29701 ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct ttcggataac
    29761 caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta tgctcaagtt
    29821 ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta ccgctggctt
    29881 tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt cccatctcgg
    29941 tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga cagtaccagt
    30001 ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct tcctgtggcg
    30061 taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa tagttatcga
    30121 aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg tcctgccagg
    30181 caatgttgat atgcatgagg aaaatctcca gagtgaagca atttgcgcga cagggtagca
    30241 taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat cagttgcggg
    30301 ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg acgctggtaa
    30361 cgccgccagg cctgtacgcc attataaatg gcgctaccca gcatcgtcag tagcagcagc
    30421 gtggtgctga gatagcgcca caggccggaa cgatccggga tcggatgaag gcctatatgc
    30481 tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt aaacggcgta
    30541 tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg cgggtagtcg
    30601 taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc gctgacaatc
    30661 aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt ttcatgcgtg
    30721 ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact caccgggcgt
    30781 aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag cgcgtcccag
    30841 tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc atctttggca
    30901 gaacacagat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa taccatgccg
    30961 gatttttgaa ttgctgaacg taatgaggcg ctgacgcgag attcatcttc cggtttaggg
    31021 tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag ttcagattct
    31081 ggtaatggta atgagcgggc gtcgttccag atgatctgcg agcagtcaaa cggtaaaaaa
    31141 ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat tcccgtaccg
    31201 ctaatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt tacgctggtg
    31261 gcttcaatgg tctgcgcacc tttcatccat gagagagtga atttcagcgg catatccagc
    31321 gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat aatcgtactg
    31381 cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag atatcgcccc
    31441 tgacgcacta ggtggcggtc gagatagata tcaatatcgg tttgttgacc gagatcctga
    31501 gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag tgaaatattg
    31561 ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt accgcgcaga
    31621 caatgaattt cccgcaggga ggattttgcc gggggggcga atagccccca cagacctgcg
    31681 cccagcagca gtaacgcgcc gcctgccagc cacggaacaa atacatccgg ggtaatcagg
    31741 cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag cccgcgcgga
    31801 cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc gatttgctca
    31861 ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc atagccgcga
    31921 gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag cggcagcgaa
    31981 tcggtatgaa tcagttcaac ggtattctca tcgttgatgt actgctccca gaaagggggc
    32041 aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc ggtagagatg
    32101 ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc attcagcgcc
    32161 agagagatcg gtgccgcgct ggctcccgtt gggccaggca cctgtaatac ctgcgtcagg
    32221 ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg cgtttgcgca
    32281 tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt tatcagccat
    32341 cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt gctcatgctt
    32401 tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cggacacaat acatgattct
    32461 gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac atcacagtaa
    32521 ccgctgatca tagcaaaccc tctgaaggcg gaatatcagc aaattcctgg agttatttca
    32581 acctattgac ttttaatttg atttgaggac gattatttat taagttacat aaatgtaaat
    32641 aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg ctgatttcac
    32701 agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa tgttgaaact
    32761 gtagcccgtt cccgactgtt taccgtcgag agcgtggatc tagagttcag caatggcgtg
    32821 cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat tgtgccgatt
    32881 gtggacgatc acctgatcct gatccgcgaa tatgcagtgg gaactgaatc ctacgaatta
    32941 ggtttttcga aaggattaat tgatccgggt gaaagcgtct tcgaagccgc taaccgcgag
    33001 ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa gctcagcatg
    33061 gcaccgtctt acttttccag caaaatgaat atcgtggtag cgcaagatct ctacccggaa
    33121 tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct ggcgcatatg
    33181 atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc gctgttcctc
    33241 gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg cctgatgcac
    33301 aatgcttatc aggcttatgg ggttcctgca atatattgag ttttcacgat tttgttagcc
    33361 ggataacgcg ttcacccgta tccggcataa acaagtgcac tttgtcagca aactgaaaag
    33421 gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct cgccattatc gataatggtc
    33481 gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa atactcttcg
    33541 cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac cgtcacaata
    33601 cccggcggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt catataagcg
    33661 tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg atctttaatc
    33721 gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc aatccagacc
    33781 gaggtcacaa cgcccggtcc gtaacccgag aaccacgcat ctttcgaact gttagtggta
    33841 ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca gccggtaccc
    33901 tgccagcctg gctcaccaaa gatattggtg ttcaaagcac tcttaatcag gaatgccagc
    33961 ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac taacgcctga
    34021 tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg ggagatagcg
    34081 acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc gtaaatcacc
    34141 ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac gccgccctga
    34201 tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt cgccatgacc
    34261 gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc cagcgattcg
    34321 gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc agcgtagtcg
    34381 acgcccatcg cccgcattgc gcgtaccatc accacgtttt tcgactgacc cagcccctga
    34441 cgtaagcgaa ttggaccggc atactgcggt ggtgagtttt tcggctgcca gtcagaaccg
    34501 gcacctgcgt cccagcgaga aattggcaca tcgttcaaca tacttgccag cgtcagacct
    34561 ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg acgcagtgcc
    34621 tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag cgccataacg
    34681 gcaccatttt gcggattgat cgacaccagt gccgagttca cttccggcac ttgtgccagc
    34741 caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag aacatcggtc
    34801 actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc ccagcgaacg
    34861 ccttccatac tcaatgcgac ggtcgacccg tccgccagca ttgccgtcgc ttcctgagga
    34921 ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc cttcagcgta
    34981 tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac atttgccggg
    35041 ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc ctgctgcgcg
    35101 gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc ataggcactt
    35161 tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg cgcagaaaaa
    35221 gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg atcgaactgt
    35281 tgctgggtga tatatccttc atccagcatc cgcgatagca cgacgttacg ccgcgcgacg
    35341 gcacgatcca tcgagtagag cgggttgaag gtggaaggcg ctttcggcag cccggctatc
    35401 accgccattt cgttcagcgt cagttggtcg accgtttttc cgaaatagac ttgtgccgca
    35461 gcaccgacac cataggcgcg gtaaccaagg taaatcttgt tcagataaag ctcgaggatc
    35521 tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt aatcttacgc
    35581 atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt aatggtactt
    35641 gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa gatccccacc
    35701 gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa ggctttcacc
    35761 atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc gtattgagca
    35821 atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac atcttttaat
    35881 gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat cgagcctgct
    35941 cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt cactggaaat
    36001 ttcccattta gtttcatttg ggcagtttat aaacaaacgc gcggtagtat aaaggcaagc
    36061 cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca caatggcatt
    36121 taagatctgg caaattggtt tgcatttaca acagcaagaa gcggtagcgg ttgctattgt
    36181 gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg cgcacgacat
    36241 tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt taccgtggag
    36301 tcgcgaactg ccgcagcgcc atcacatcat gttggcgttt cccgccagtc gcacattaca
    36361 gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg cctggctgtc
    36421 cgggacgatg gctcgcgagc tggatatgga tccggactcc ctgcgcttcg attacagcga
    36481 agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc tggcaacgct
    36541 gcttactttg gcagaaaggt tgcgtgttca tgttagtgcg atcaccccgg atgccagtgc
    36601 attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct ggcgtgataa
    36661 cgaacagtgg ctgtgggcga cgcgctatcg ctgggggcgc aaactggcgg tggggatgac
    36721 tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg cgatatgtgg
    36781 cgaaggcgga tttgatcctt gggaggccgt ttctgttcgt cagccgccgt taccgccgcc
    36841 aggtggagac tttgccatcg cgttgggctg gcgcttggga aggcgtactg atgaacccgc
    36901 caattaattt tttgccctgg cgacagcaac gccggaccgc ttttcttcgt ttctggttgc
    36961 tgatgttcgt tgcgcctctg ctgctggccg tcgggataac gctaatactg cgtctgacaa
    37021 gcagcgccga agctcgcgta gacgccgttt tgcttcaggg ggaacaacaa ctcgcccgca
    37081 gcttacagat aaccaaacca cgtttgctgg agcgccaaca aattcgtgag cagcgtttgc
    37141 aaaggcagcg ccagcgacaa tttacccgcg actggcaatc tgcgctggaa gcactggcgg
    37201 cgcttttacc cgagcacgcc tggctgacaa cgataagctg gcagcaggga acgctggaga
    37261 tcaaggggct tacaacaagc attaccgcgt taaacgcact agaaacgtca ctccgccagg
    37321 atgcatcttt tcatctcaat cagcgggggg ccacgcagca ggatgcgcag ggacgctggc
    37381 aatttgagta tcagttaaca aggaaggtta gcgatgaaca tgttctttga ctggtggttc
    37441 gccacatcac cccgcctccg ccagttttgc tgggcattct ggttgctgat gttagttacg
    37501 ctcatttttc tgtcatcgac acaccatgaa gagcgcgacg cattaattcg actacgggca
    37561 agtcatcatc agcattgggc cacactgtat cgcctggttg acaccactcc cttcagcgaa
    37621 gaaaaaacgc tgcccttttc gccactggat tttcagttgc ccggcgcaca actggttttc
    37681 tggcatccat ccgcgcaggg aggcgagttg gcgttgaaaa cgctgtggga agcagtgccg
    37741 tcggcattta cacggctggc agagcgcaac gtcagcgtga gccgtttttc attaagcgtg
    37801 gaaggtgatg atcttttgtt caccctacaa ctggagacgc cgcatgaggg ttaaacgctg
    37861 gttgttggca ggtattgcat tgtgcctttt aaccggtatg cgtgaccctt ttaaaccgcc
    37921 ggaagatcta tgccggatta gcgaacttag ccagtggcgc tatcagggga tggtagggcg
    37981 aggcgagcgc atcatcggtg taataaaaga cgggcaaaag aaatggcgac gggtgcagca
    38041 aaacgatgtg ctggaaaacg gctggacaat tttacagctg acgccagacg tactaacgct
    38101 gggtaccggg acaaactgcg aaccgccaca atggttgtgg caacggcaag gagatacaaa
    38161 tgaagcaatg gatagccgca ctactgttga tgctgatacc cggcgtacag gcggcaaagc
    38221 cgcaaaaagt gacgctgatg gtggatgacg ttccggtagc tcaggtgttg caggcgctgg
    38281 ctgaacagga gaagttgaac ctggtggtgt cgccagacgt cagcggtacg gtgtcgttac
    38341 atctaacaga tgttccctgg aagcaggcac tacaaactgt agtgaaaagc gccggactga
    38401 taacgcgcca ggagggcaac attctctcag tgcattccat tgcctggcag aatgacaata
    38461 tcgcccgtca ggaagcggag caggcgcggg cgcaggcaaa tctgccgctg gaaaatcgca
    38521 atattactct gcaatacgcc gacgccggag agctggcgaa agctggggag aagctactga
    38581 gtgccaaagg gagtatgacc gtcgataaac gcaccaatcg ccttttgcta cgagataaca
    38641 aaacggcgtt aagcgccctt gaacagtggg tagcgcaaat ggatctgccg gtcgggcagg
    38701 ttgagctgtc ggcgcatatt gtcaccatta atgaaaaaag tttgcgtgag ttaggcgtga
    38761 aatggacgct ggccgatgcg caacacgctg gtggcgttgg gcaagtcacc acgcttggta
    38821 gcgacctctc cgtagcgacg gcgacaacgc atgtcggttt taacattggg cgcatcaacg
    38881 gacgtttgct ggatcttgag ctttccgcgc tcgaacaaaa acagcagctg gatattatcg
    38941 ccagtccgcg tctgctggcc tcacatcttc agcctgccag cattaaacag gggagcgaaa
    39001 ttccatatca ggtttccagc ggggaaagtg gcgcgacgtc ggtggaattt aaagaggccg
    39061 tcctggggat ggaggtcacg cccacggtgt tacaaaaagg tcgcatccgg ctgaaattac
    39121 acatcagcca gaacgttccg gggcaggtgc tacagcaggc cgatggcgaa gtgctggcga
    39181 ttgataagca ggagattgaa acgcaggtcg aggtcaaaag cggagaaacg ttggcgctgg
    39241 gcggcatttt tacccgtaaa aataaatcgg gtcaggatag cgtgccgttg cttggcgaca
    39301 ttccctggtt cgggcaatta tttcgtcatg acggaaaaga agatgaacga cgcgagttag
    39361 tggtgtttat cacgccacga ctggtttcca gtgagtaaac agccgtaaaa gcggtaatgt
    39421 ttttacgctg aacgtgtttc atctatttga cgcgcgcagg tatttagcat acaaggagta
    39481 ccgatttgag agttggtgct cttcgctgcc tgccttccat gatgatgatt tatcattcag
    39541 gcggcatttt gctgtctttt ttacgctaat cttacccggt gatttatcgc cagagcggtg
    39601 gtagcaaggc agcgcgcttg cagcgaccag atatgcagag ggatgggtga tttattcagt
    39661 tgccaaaccc gctggagtat tgagataatt ttcagtctga ctctcgcaat atcttatgag
    39721 gtttcagttc atgtcctgcg gagctctctg agcgaagcgg gtttatcatt aacgaatagt
    39781 cttagtagta ccgaaaaaat ggcagagaaa cgcaatatct ttctggttgg gcctatgggt
    39841 gccggaaaaa gcactattgg gcgccagtta gctcaacaac tcaatatgga attttacgat
    39901 tccgatcaag agattgagaa acgaaccgga gctgatgtgg gctgggtttt cgatttagaa
    39961 ggcgaagaag gcttccgcga tcgcgaagaa aaggtcatca atgagttgac cgagaaacag
    40021 ggtattgtgc tggctactgg cggcggctct gtgaaatccc gtgaaacgcg taaccgtctt
    40081 tccgctcgtg gcgttgtcgt ttatcttgaa acgacgatcg aaaagcaact tgcacgcacg
    40141 cagcgtgata aaaaacgccc gttgctgcac gttgaaacac cgccgcgtga agttctggaa
    40201 gcgttggcca atgaacgcaa tccgctgtat gaagagattg ccgacgtgac cattcgtact
    40261 gatgatcaaa gcgctaaagt ggttgcaaac cagattattc acatgctgga aagcaactaa
    40321 ttctggcttt atatacactc gtctgcgggt acagtaatta aggtggatgt cgcgttatgg
    40381 agaggattgt cgttactctc ggggaacgta gttacccaat taccatcgca tctggtttgt
    40441 ttaatgaacc agcttcattc ttaccgctga aatcgggcga gcaggtcatg ttggtcacca
    40501 acgaaaccct ggctcctctg tatctcgata aggtccgcgg cgtacttgaa caggcgggtg
    40561 ttaacgtcga tagcgttatc cttcctgacg gcgagcagta taaaagcctg gctgtactcg
    40621 ataccgtctt tacggcgttg ttacaaaagc cgcatggtcg cgatactacg ctggtggcgc
    40681 ttggcggcgg cgtagtgggc gatctgaccg gcttcgcggc ggcgagttat cagcgcggtg
    40741 ttcgtttcat tcaagtcccg acgacgttac tgtcgcaggt cgattcctcc gttggcggca
    40801 aaactgcggt caaccatccc ctcggtaaaa acatgattgg cgcgttctac cagcctgctt
    40861 cagtggtggt ggatctcgac tgtctgaaaa cgcttccccc gcgtgagtta gcgtcggggc
    40921 tggcagaagt catcaaatac ggcattattc ttgacggtac gttttttaac tggctggaag
    40981 agaatctgga tgcgttgttg cgtctggacg gtccggcaat ggcgtactgt attcgccgtt
    41041 gttgtgaact gaaggcagaa gttgtcgccg ccgacgagcg cgaaaccggg ttacgtgctt
    41101 tactgaatct gggacacacc tttggtcatg ccattgaagc tgaaatgggg tatggcaatt
    41161 ggttacatgg tgaagcggtc gctgcgggta tggtgatggc ggcgcggacg tcggaacgtc
    41221 tcgggcagtt tagttctgcc gaaacgcagc gtattataac tctgctcacg cgggctgggt
    41281 taccggtcaa tgggccgcgc gaaatgtccg cgcaggcgta tttaccgcat atgctgcgtg
    41341 acaagaaagt ccttgcggga gagatgcgct taattcttcc gttggcaatt ggtaagagtg
    41401 aagttcgcag cggcgtttcg cacgagcttg ttcttaacgc cattgccgat tgtcaatcag
    41461 cgtaacaaca agaaaggtca ggccgcttat caagcggtct attagcttca ggttaattgc
    41521 aacgtggtaa gcattaacct tttagtgggg tgttaaatgg atgaattcaa accagaagac
    41581 gagctgaaac ccgatcccag cgatcgtcgt actggtcgtt ctcgtcaatc ttctgaacgt
    41641 tctgagcgta ctgaacgtgg cgaaccgcag atcaattttg atgatattga acttgatgac
    41701 actgacgatc gccgtccgac tcgtgcgcaa aaagagcgca atgaggaacc ggaaatcgaa
    41761 gaagaaattg acgaatccga agatgaaacc gtggatgaag agcgcgtaga gcgtcgtccg
    41821 cgtaagcgca aaaaagcagc cagtaaaccc gcttctcgtc agtatatgat gatgggcgtc
    41881 ggcattctgg ttctactgct gttgatcatc ggtatcggtt ctgcgctaaa agccccctcg
    41941 accacttcca gcgatcaaac cgcgtctggc gagaagagta ttgatcttgc tggcaatgcg
    42001 accgatcagg cgaatggcgt gcagccagcg ccgggaacca cgtctgcgga aaatactcag
    42061 caggatgttt ctctgccacc gatctcttct acgccgactc aagggcaaac cccggcggca
    42121 acggatggtc aacaacgtgt tgaagtgcag ggtgacctga acaatgcgct gacccagcca
    42181 caaaatcagc aacagttgaa caatgtggcg gtcaattcta cattgccgac tgaaccagca
    42241 actgtcgcgc cagtacgcaa tggcaatgca tcgcgtgaca cggcgaaaac gcaaactgct
    42301 gaacgtccgg ccactacgcg tccagctcgt cagcaggcgg tgattgaacc gaaaaaaccg
    42361 caagcaaccg tgaaaacgga accgaagccg gtagcacaga cgccgaagcg tactgaacca
    42421 gctgctcctg tggcgagcac gaaggcaccg gctgcgactt ctacgccagc accaaaagag
    42481 acggcgacta cggctccagt acagacggca tccccggcgc aaaccacggc aacaccagcc
    42541 gctggaggga agaccgcagg taatgtgggt tcgttgaaat cggcaccgtc cagccattac
    42601 actctgcagc tgagcagttc ctctaactac gacaacctga acggttgggc gaagaaagag
    42661 aatctgaaaa actacgttgt ctatgaaacg acgcgtaatg gtcagccgtg gtatgtcctg
    42721 gtttctggcg tgtacgcttc gaaagaagag gcgaaaaaag cggtatctac attgccagca
    42781 gatgttcagg ccaaaaaccc gtgggcgaaa ccgctgcgtc aggtacaggc cgatctgaag
    42841 taatcaaggt tatctcccgc aatggtttat cgttgcggga gttgcctgaa gcgctggatg
    42901 ctgtcggagc tttctccaca gccggagaag gtgtaattag ttagtcagca tgaagaaaaa
    42961 tcgcgctttt ttgaagtggg cagggggcaa gtatcccctg cttgatgata ttaaacggca
    43021 tttgcccaag ggcgaatgtc tggttgagcc ttttgtaggt gccgggtcgg tgtttctcaa
    43081 caccgacttt tctcgttata tccttgccga tatcaatagc gacctgatca gtctctataa
    43141 cattgtgaag atgcgtactg atgagtacgt acaggccgca cgcgagctgt ttgttcccga
    43201 aacaaattgc gccgaggttt actatcagtt ccgcgaagag ttcaacaaaa gccaggatcc
    43261 gttccgtcgg gcggtactgt ttttatattt gaaccgctac ggttacaacg gcctgtgtcg
    43321 ttacaatctg cgcggtgagt ttaatgtgcc gtttggccgc tacaaaaaac cctatttccc
    43381 ggaagcagag ttgtatcact tcgctgaaaa agcgcagaat gcctttttct attgtgagtc
    43441 ttacgccgat agcatggcgc gcgcagatga tgcatctgtc gtctattgcg atccacctta
    43501 tgcaccgctg tctgcgaccg ccaactttac ggcgtatcac acaaacagtt ttacgcttga
    43561 acaacaagcg catctggcgg agatcgccga aggtctggtt gagcgccata ttcctgtgct
    43621 gatctccaat cacgatacga tgttaacgcg tgagtggtat cagcgcgcaa aattgcatgt
    43681 cgtcaaagtt cgacgcagta taagcagcaa cggcggcaca cgtaaaaagg tggacgaact
    43741 gctggctttg tacaaaccag gagtcgtttc acccgcgaaa aaataattct caaggagaag
    43801 cggatgaaac agtatttgat tgccccctca attctgtcgg ctgattttgc ccgcctgggt
    43861 gaagataccg caaaagccct ggcagctggc gctgatgtcg tgcattttga cgtcatggat
    43921 aaccactacg ttcccaatct gacgattggg ccaatggtgc tgaaatcctt gcgtaactac
    43981 ggcattaccg cccctatcga cgtacacctg atggtgaaac cggtcgatcg cattgtgcct
    44041 gatttcgctg ccgctggtgc cagcatcatt acctttcatc cagaagcctc cgagcatgtt
    44101 gaccgcacgc tgcaactgat taaagaaaat ggctgtaaag cgggtctggt atttaacccg
    44161 gcgacacctc tgagctatct ggattacgtg atggataagc tggatgtgat cctgctgatg
    44221 tccgtcaacc ctggtttcgg cggtcaatct ttcattcctc aaacactgga taaactgcgc
    44281 gaagtacgtc gtcgtatcga cgagtctggc tttgacattc gtctggaagt ggacggtggc
    44341 gtgaaggtga acaacattgg cgaaatcgcc gcggcgggcg cggatatgtt cgttgccggt
    44401 tcggcaatct tcgaccagcc agactacaaa aaagtcattg atgaaatgcg cagtgaactg
    44461 gcaaaggtaa gtcatgagta agtttgaaga tattcgcggc gtcgcttttg atctcgacgg
    44521 tacgctggtg gacagtgctc ccggtcttgc tgctgcggta gatatggcgc tgtatgcgct
    44581 ggagttgccc gtcgcaggtg aagaacgcgt tattacctgg attggtaacg gcgcagatgt
    44641 tctgatggag cgcgcattga cctgggcgcg tcaggaacgt gcgactctgc gtaaaacaat
    44701 gggtaaaccg cccgttgatg acgacattcc ggcagaagaa caggtacgta ttctgcgtaa
    44761 actgttcgat cgttactatg gcgaggttgc cgaagagggg acgtttttgt tcccgcacgt
    44821 tgccgatacg ttgggcgcgt tgcaggctaa aggcctgccg ctaggcctgg tcaccaacaa
    44881 accgacgccg ttcgtcgcgc cgctgctcga agccttagat atcgccaaat acttcagcgt
    44941 ggtgattggt ggtgatgatg tgcaaaacaa aaaaccgcat ccggacccgc tgttactggt
    45001 ggctgagcgg atgggaattg ctccacaaca gatgctgttt gttggcgact cacgcaatga
    45061 tattcaggca gcaaaagcgg caggttgccc atcagttggc ttaacctacg gatataacta
    45121 cggcgaggct atcgatctca gccagcctga tgtaatttat cagtctataa atgaccttct
    45181 gcccgcatta gggcttccgc atagcgaaaa tcaggaatcg aaaaatgact aagcccatcg
    45241 tttttagtgg cgcacagccc tcaggtgaat tgactattgg taactacatg ggtgcgctgc
    45301 gtcagtgggt aaacatgcag gatgactacc attgcattta ctgtatcgtt gaccaacacg
    45361 cgatcaccgt gcgccaggat gcacagaagc tgcgtaaagc gacgctggat acgctggcct
    45421 tgtatctggc ttgtggtatc gatcctgaga aaagcaccat ttttgttcag tcccacgtac
    45481 cggaacatgc gcagttaggc tgggcactga actgctatac ctacttcggc gaactgagcc
    45541 gcatgacgca gtttaaagat aaatctgcgc gttatgccga gaacatcaac gctggtctgt
    45601 ttgactatcc ggtgctgatg gcagcggaca tcctgctgta tcaaactaat ctggtaccgg
    45661 tgggtgaaga ccagaaacag cacctggaac tgagccgtga tatcgcccag cgtttcaacg
    45721 cgctgtacgg cgatatcttt aaggtgccgg agccgtttat tccgaaatcc ggcgcgcgcg
    45781 taatgtcgct gctggagccg accaagaaga tgtccaagtc tgacgataac cgcaataacg
    45841 ttattggcct gctggaagat ccgaaatcgg tagtgaagaa aatcaaacgc gcggtcaccg
    45901 actccgacga gccgccggta gttcgctacg atgtgcagaa caaagcgggc gtttccaacc
    45961 tgctggatat cctttctgcg gtaacgggcc agagcatccc ggaactggaa aaacagttcg
    46021 aaggcaagat gtatggtcat ctgaaaggcg aagtggctga tgccgtttcc ggtatgctga
    46081 ctgaattgca ggaacgttac caccgtttcc gcaacgatga agccttcctg caacaggtga
    46141 tgaaagacgg cgcagaaaaa gccagcgcgc acgcttcccg tacgctaaaa gcggtgtacg
    46201 aagcgattgg ttttgtggcg aagccgtaat atcaaaacgc tcggacggtc ccctcgcccc
    46261 tttggggaga gggttagggt gaggggaacg tgcgggatgt aaaattcagt gcgccggcat
    46321 cgccggatgc gatgctggtg catcttatcc ggcctacggt taatgtcttt tattatcaat
    46381 tgttaaggtg atgcggttgg cgcggcagaa gagttcggag acgtgcaccg gtttgtcatt
    46441 ctgatcaaag gcgattttgc tgatgcgaaa caacggttcg cccagttcgc atttcagcca
    46501 ttttgcctgc gggcgggtgg cggcaaagat atcgatggtc tttttgtcgc tgaccactcg
    46561 cgtgtcaaaa cgctcctgaa ataactgata ggtggagctt ccttcgacgt aaatctcatc
    46621 aaagtcagga taacgcgaca gcgggatcca cgaactatca ataaatagcg gctctttatc
    46681 gagatacatc acccggcaaa gatggaacac ttcgctgtta ccggggatat tgagcttttc
    46741 gcaaaacggc gcggcgctga tccgttcttg ctcgatcact ttctctttcg tcgccttgcc
    46801 ttgtgagacg ccaaaatcgg taaaaccact gacggtcagc agggcgtttt cgaccttctg
    46861 gctttgtaca aaggtgcctt tgccctgcca gcggatcagt acgccgtctg ccactaagtc
    46921 gctgatggct ttgcgaatgg taatgcggct gacgttatat tgtgtacaaa gctcattttc
    46981 ggtagggatc tgttgcccgg cctggtaaac cccctgcgcg atatcatcca gcagccgctg
    47041 gcggacggta gcgtagagga gttgatgaga gtagcggtcc gtagctgaca taatcgaccc
    47101 tgaaataaag aatgaattac acggattata gggtcgtttt ccggcaagta acagcacgtt
    47161 ggcgtgaaga tgctcacgcc aacgttatgc ctaccaggca ccgtggtact gaatagtttg
    47221 tgccgcgcac gccgttccct gcgccatcgc ctgcggtaat gtcatccccg cagaccagcc
    47281 gcaaaggaat ccggcaatga acgaatctcc ggcacccatg gtgtcgataa ccgtcacctg
    47341 ttcaggagcc tgacgccaga actgcacgcc atcccaggca atactgccgt tttcacccaa
    47401 cgtgacaatc accacgcctg cgccacgggc aacaatcgct ttcatcttca gacgcagcgc
    47461 ttcgtcttct tgcggtgcgg aggcaaaggc aaaatcgaga tgcggcacca gtgtctgcca
    47521 gagcgggctg tcccacttgt cggagacgtc gaaagcggta agtttgcccg cagcgtgcag
    47581 ctgtgggaat gcgtcttccg catgtcccca gattgccgcg tgcacaatgt catactgcgc
    47641 cagctaggcg taatcctctt cactcagggc aaagtcggcc atcacgcctt cggtgtagtc
    47701 gccaaaaacg cgatcattgt cgtgcagttc cacctgagtt tgtgcggtaa cgccgtgttt
    47761 cgtatggaca tggctgatat cgacgcccat gctggcgaga tcctgcttaa gctttgtgcc
    47821 gtagtcatcg tcaccaaccc aggtaatgca tcccggcttt ataccgtagc gagtgcagta
    47881 caccgccacg ttcaccgcat taccgccaga aaacgcttta ttcagttgcg ggtagatatc
    47941 gacgcagtta tcgccgattg tcgccagggt tttcatcact catcctccgg cagcagcgcg
    48001 cgaaagcgtt ccagcgcctg gcgggcgtaa agtctgggct cgttcatata catcgttacc
    48061 agttccactg tgcagtatcc ctcatagccc cgatcaataa tatcgcgcat cagttcccgc
    48121 agcggcattt tgccttcgcc gggaatgtaa tgcgtgtcgc tggccccgtc gctgtcgaca
    48181 atatgcagat gacgtaattt atcgcccagt ttgtcgaaat aactcatcac cggttccgcc
    48241 tggacatacg gcgcgcaaat gtcgaccatg ctgaacaggc gcggcgaagg caccagcgcc
    48301 agcgcatgaa gcacatcatt agcattacac acgacgttcg attcatacgg cgttaacggt
    48361 tcgaggatca gatccatgcc gatgttttcc gcgtactcac acagttcgct taagttctct
    48421 gccagccgcc cccagataac gttaggcggc gtgagatagc ccgcgtgggc cgcagaaatc
    48481 agcgtataac ccgcgttcat ctcttttgcc atatccatcg ccagcttgat catgtccagg
    48541 ctttcacgac gcatatgttc atcgcccagc atcatgttat acggatagcc gttggtttct
    48601 ggcgtgtaac cgataatcgg catctgatac gtctgcgcca gcgccttgat ttgtttgatg
    48661 ccgcccgctt ttaagtccgg cgcgaacgcg tgcgggcggc cgccccaaat ttcgatgcca
    48721 tcgtaaccca gctcgcttgc atcacggaat gcatgttcaa taggcagccg ctggtggccg
    48781 caggtaaaca tacctgtttt catcgggtaa ctccttgcgt aaatccggcc cgttgccggg
    48841 ccgggagaga ggattaatat tccaccagac caccgtaata gcggcgttca tccgggttgt
    48901 gatctttgta aatagacagg tagtagcaga gccactccat tggcacgaac atcaggaacg
    48961 gtgccagcca cgggtgcagt ccttgcgaaa ttgcggcgta atcgatgacg atcacgttgt
    49021 cagtacgctg tttaacaaag ttaatggcgc gttcggtggt gtggcgactt tcatcattgc
    49081 cgagcaggaa caggaacgga acgcccggtt cgacaatctc cagcgggccg tgacggaact
    49141 caccgctctc aatcacacag ccgtgcgtcc aggtaaattc catcagcgtc acgatgcctt
    49201 ctttgtaacc cagcgggcgc agcgggcctg cagcaaccgt gtaaatcatc ggccactggc
    49261 tggccagttc accaagctgg cggccttttt cttcccaagt gcgtaccaga taaccgagcg
    49321 cattcggcag ctgtttgaga tcgtttttga tcttgccgat ttccgcgtgc ggcgcgaggc
    49381 gggtgatcat ctccagcacc acgctgtagc agagcagcag atgaatttcc cagatacagt
    49441 cggcctgata atcaatgcta aattccgccg ccgaggtaat cgggctatcc gcgcgtttgg
    49501 tgaacgccgc agtgagtgcg ccgcaggccc ggcccagctc cagcgctttg attacctctt
    49561 cggttttacc gtagtcagaa acgccaatca ccgcgcaacg agcgtcgagg cgatatgggg
    49621 tgttatcgca gaactcccag ccggaaatgg cgtaaacctg aagatcggaa aagcgatctg
    49681 ccagatgttt cgccgtttgc gccgcgttga gtggcgatcc gcaggcaacg aaataaatac
    49741 gatcgatgtc gcgcttcacc atctcttcca cgatggtgtg caccagcgga acgtcatggc
    49801 tgagaacttt ttccacttcc tgaaccatat tttcggtgac cagaaagtcc acagtgcttt
    49861 tatcaatatc caacatttta ttttctccag ataacattac gacaaagcgt tgagctggcg
    49921 gctacgcttc gcccagaagg cgtaagcagg cagaccagtg gcaataacga tgacggcgca
    49981 gataaggccg ggaatcggtg cccagacaaa ggttgaggcg accagaatga ggcttgacgc
    50041 aatggcgagg ggggtcatca gcccgaaagc cggagtacgc cacagcggtt tgtaatcgtc
    50101 gcgtttacga caccagatga tggagccgaa ggtgagggta tttttgaaac acattaccag
    50161 ggtgaaatag cccagcaggc tggtgagatc ggaaacgaag atgaagaaga tccccagcgc
    50221 cccttgcaaa atgatggaga catccggcgt gttgtatttc ggatgcacat ggccgaagca
    50281 tttgaagaac aggttgtctt tcgccatcgc gtattccagc cgcggctggt acatcacgca
    50341 gctggaaagc gaaccaagaa tgacgatcat cgccgtgatg gcaacaaaga tcccagcggt
    50401 gctgccgagt gcggggatcc aggtcagggc gtcggaaatc ggcgtttcag aattggcgag
    50461 tttgtcgaag ggcatcaggc cggaaatcac cagcgccagc agggtgtaga gcaccagaac
    50521 cagcagacag gaaccaatca gcgctcgtgg catggttttg ccggggtttt taatttcgcc
    50581 ggtcatataa cagatagagg ccatgccggt atacgaccaa ctggtggcag agatccccgc
    50641 cagcagcgcc ataaagctgc ccgttgcgcc aatcgcagtg gtagcagggg cggcaaaatt
    50701 ctccgcttta aaccagaaga tcccaagacc aatgacgata gtgaacggga taattttggc
    50761 gatggtaatt agcgtctgaa acgctgcgcc gccttcaacg gagcgcaggt gcagcagcat
    50821 aaaggcgata attaatccgg cggcgataaa tttaccgagc aacggatcga taggcgttaa
    50881 aaagccaaga ttgctgacaa tcgccagcgc cataatcgac aatgacggcg catcgttggc
    50941 ccagaagctg gcccagccgg agaggaaagc cagcggtcgg cttccggcat ttttcagata
    51001 aacataatct gcgccatttt ccggataagc ggtggatagt tccgcatata cgcacatttg
    51061 cgggatcaca attaacccgc cgatgacaaa tgcgagcacc gtcagccacg gcgtgcccgc
    51121 cgcttttgcc acttcaccca cagatacaaa aataccggag ccgacggttg tcccgacggc
    51181 gattgcaaga acggcccaaa atccgagctt gcgttggagt tcctggcttc ccatagcgat
    51241 acctttttct tgtccaacac gttgtagggg agagtttgca gtcatttaaa aggtgacatg
    51301 acaatgcatg atgaatataa caacatataa tgttatgtaa tgtggaggaa gatcacataa
    51361 tttcgcacag gatcgcgctg tggctgatga atgtggttat ccaattgaaa ttaaagtgaa
    51421 aatattttta cgggtgggga gcaagaagga tatagaaaca aatacgccct cgggtaatcc
    51481 cgagggcgtc aggcaattaa atcagcgtta gctgatttct gtcagagact tactgtgcag
    51541 taggaccgca accgccaatg attttagaaa tagaaattgc tgggtgcagc aggtagtcgt
    51601 aagagcagtt cttatcacgg ttttcaatat gaccagtaca tgcagttaac gtagccagta
    51661 ctgcacctac cagtgcaact ttaataaatt tgttca
//
LOCUS       DAZFBR010000020        50026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFBR010000020 DAZFBR010000000
VERSION     DAZFBR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.020"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            227..2368
                     /locus_tag="ACHJZ2_001351"
     CDS             227..2368
                     /locus_tag="ACHJZ2_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO4567498.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPHHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2432..4324)
                     /gene="parE"
                     /locus_tag="ACHJZ2_001352"
     CDS             complement(2432..4324)
                     /gene="parE"
                     /locus_tag="ACHJZ2_001352"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO4567499.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4353..4934)
                     /gene="yqiA"
                     /locus_tag="ACHJZ2_001353"
     CDS             complement(4353..4934)
                     /gene="yqiA"
                     /locus_tag="ACHJZ2_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO4567500.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4934..5761)
                     /gene="cpdA"
                     /locus_tag="ACHJZ2_001354"
     CDS             complement(4934..5761)
                     /gene="cpdA"
                     /locus_tag="ACHJZ2_001354"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO4567501.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5786..6208)
                     /gene="yqiB"
                     /locus_tag="ACHJZ2_001355"
     CDS             complement(5786..6208)
                     /gene="yqiB"
                     /locus_tag="ACHJZ2_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO4567502.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAHAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6209..6838)
                     /gene="nudF"
                     /locus_tag="ACHJZ2_001356"
     CDS             complement(6209..6838)
                     /gene="nudF"
                     /locus_tag="ACHJZ2_001356"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO4567503.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7043..8524
                     /gene="tolC"
                     /locus_tag="ACHJZ2_001357"
     CDS             7043..8524
                     /gene="tolC"
                     /locus_tag="ACHJZ2_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO4567504.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8672..9343
                     /gene="ygiB"
                     /locus_tag="ACHJZ2_001358"
     CDS             8672..9343
                     /gene="ygiB"
                     /locus_tag="ACHJZ2_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO4567505.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9349..10509
                     /gene="ygiC"
                     /locus_tag="ACHJZ2_001359"
     CDS             9349..10509
                     /gene="ygiC"
                     /locus_tag="ACHJZ2_001359"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO4567506.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(10547..11335)
                     /gene="ygiD"
                     /locus_tag="ACHJZ2_001360"
     CDS             complement(10547..11335)
                     /gene="ygiD"
                     /locus_tag="ACHJZ2_001360"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO4567507.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            11478..12251
                     /gene="zupT"
                     /locus_tag="ACHJZ2_001361"
     CDS             11478..12251
                     /gene="zupT"
                     /locus_tag="ACHJZ2_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO4567508.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(12309..12479)
                     /gene="yqiD"
                     /locus_tag="ACHJZ2_001362"
     CDS             complement(12309..12479)
                     /gene="yqiD"
                     /locus_tag="ACHJZ2_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO4567509.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(12741..13394)
                     /gene="ribB"
                     /locus_tag="ACHJZ2_001363"
     CDS             complement(12741..13394)
                     /gene="ribB"
                     /locus_tag="ACHJZ2_001363"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO4567510.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(13498..13646)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            13768..14067
                     /gene="ubiK"
                     /locus_tag="ACHJZ2_001364"
     CDS             13768..14067
                     /gene="ubiK"
                     /locus_tag="ACHJZ2_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO4567511.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(14102..14302)
                     /gene="glgS"
                     /locus_tag="ACHJZ2_001365"
     CDS             complement(14102..14302)
                     /gene="glgS"
                     /locus_tag="ACHJZ2_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO4567512.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            14571..15185
                     /gene="yqiJ"
                     /locus_tag="ACHJZ2_001366"
     CDS             14571..15185
                     /gene="yqiJ"
                     /locus_tag="ACHJZ2_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO4567513.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNP"
     gene            15227..16888
                     /gene="yqiK"
                     /locus_tag="ACHJZ2_001367"
     CDS             15227..16888
                     /gene="yqiK"
                     /locus_tag="ACHJZ2_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO4567514.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTVEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
     gene            17127..17186
                     /locus_tag="ACHJZ2_001368"
     CDS             17127..17186
                     /locus_tag="ACHJZ2_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4567515.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            17503..17562
                     /gene="ibsE"
                     /locus_tag="ACHJZ2_001369"
     CDS             17503..17562
                     /gene="ibsE"
                     /locus_tag="ACHJZ2_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO4567516.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(17682..19115)
                     /gene="hldE"
                     /locus_tag="ACHJZ2_001370"
     CDS             complement(17682..19115)
                     /gene="hldE"
                     /locus_tag="ACHJZ2_001370"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO4567517.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(19163..22003)
                     /gene="glnE"
                     /locus_tag="ACHJZ2_001371"
     CDS             complement(19163..22003)
                     /gene="glnE"
                     /locus_tag="ACHJZ2_001371"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO4567518.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(22026..23327)
                     /gene="ygiF"
                     /locus_tag="ACHJZ2_001372"
     CDS             complement(22026..23327)
                     /gene="ygiF"
                     /locus_tag="ACHJZ2_001372"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO4567519.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            23569..24189
                     /gene="ygiM"
                     /locus_tag="ACHJZ2_001373"
     CDS             23569..24189
                     /gene="ygiM"
                     /locus_tag="ACHJZ2_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO4567520.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            24253..25491
                     /gene="cca"
                     /locus_tag="ACHJZ2_001374"
     CDS             24253..25491
                     /gene="cca"
                     /locus_tag="ACHJZ2_001374"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO4567521.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(25672..26493)
                     /gene="bacA"
                     /locus_tag="ACHJZ2_001375"
     CDS             complement(25672..26493)
                     /gene="bacA"
                     /locus_tag="ACHJZ2_001375"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO4567522.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(26584..26952)
                     /gene="folB"
                     /locus_tag="ACHJZ2_001376"
     CDS             complement(26584..26952)
                     /gene="folB"
                     /locus_tag="ACHJZ2_001376"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO4567523.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            27058..27675
                     /gene="plsY"
                     /locus_tag="ACHJZ2_001377"
     CDS             27058..27675
                     /gene="plsY"
                     /locus_tag="ACHJZ2_001377"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO4567524.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(27688..28620)
                     /gene="ttdR"
                     /locus_tag="ACHJZ2_001378"
                     /gene_synonym="dan"
     CDS             complement(27688..28620)
                     /gene="ttdR"
                     /locus_tag="ACHJZ2_001378"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO4567525.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            28827..29738
                     /gene="ttdA"
                     /locus_tag="ACHJZ2_001379"
     CDS             28827..29738
                     /gene="ttdA"
                     /locus_tag="ACHJZ2_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO4567526.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            29735..30340
                     /gene="ttdB"
                     /locus_tag="ACHJZ2_001380"
     CDS             29735..30340
                     /gene="ttdB"
                     /locus_tag="ACHJZ2_001380"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO4567527.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            30389..31852
                     /gene="ttdT"
                     /locus_tag="ACHJZ2_001381"
     CDS             30389..31852
                     /gene="ttdT"
                     /locus_tag="ACHJZ2_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO4567528.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(31895..32908)
                     /gene="tsaD"
                     /locus_tag="ACHJZ2_001382"
     CDS             complement(31895..32908)
                     /gene="tsaD"
                     /locus_tag="ACHJZ2_001382"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO4567529.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            33146..33361
                     /gene="rpsU"
                     /locus_tag="ACHJZ2_001383"
     CDS             33146..33361
                     /gene="rpsU"
                     /locus_tag="ACHJZ2_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO4567530.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            33472..35217
                     /gene="dnaG"
                     /locus_tag="ACHJZ2_001384"
     CDS             33472..35217
                     /gene="dnaG"
                     /locus_tag="ACHJZ2_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO4567531.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            35412..37253
                     /gene="rpoD"
                     /locus_tag="ACHJZ2_001385"
     CDS             35412..37253
                     /gene="rpoD"
                     /locus_tag="ACHJZ2_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO4567532.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(37332..37838)
                     /gene="mug"
                     /locus_tag="ACHJZ2_001386"
     CDS             complement(37332..37838)
                     /gene="mug"
                     /locus_tag="ACHJZ2_001386"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO4567533.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            37963..38038
                     /locus_tag="ACHJZ2_001387"
     tRNA            37963..38038
                     /locus_tag="ACHJZ2_001387"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37996..37998,aa:Ile,seq:cat)
     gene            complement(38092..38856)
                     /gene="nfeF"
                     /locus_tag="ACHJZ2_001388"
                     /gene_synonym="yqjH"
     CDS             complement(38092..38856)
                     /gene="nfeF"
                     /locus_tag="ACHJZ2_001388"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO4567534.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            39144..39767
                     /gene="nfeR"
                     /locus_tag="ACHJZ2_001389"
                     /gene_synonym="yqjI"
     CDS             39144..39767
                     /gene="nfeR"
                     /locus_tag="ACHJZ2_001389"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO4567535.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(39921..41441)
                     /gene="aer"
                     /locus_tag="ACHJZ2_001390"
     CDS             complement(39921..41441)
                     /gene="aer"
                     /locus_tag="ACHJZ2_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO4567536.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            41859..43238
                     /gene="ygjG"
                     /locus_tag="ACHJZ2_001391"
     CDS             41859..43238
                     /gene="ygjG"
                     /locus_tag="ACHJZ2_001391"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO4567537.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(43280..43612)
                     /gene="ygjH"
                     /locus_tag="ACHJZ2_001392"
     CDS             complement(43280..43612)
                     /gene="ygjH"
                     /locus_tag="ACHJZ2_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO4567538.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            43831..44814
                     /gene="ebgR"
                     /locus_tag="ACHJZ2_001393"
     CDS             43831..44814
                     /gene="ebgR"
                     /locus_tag="ACHJZ2_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO4567539.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            44998..48090
                     /gene="ebgA"
                     /locus_tag="ACHJZ2_001394"
     CDS             44998..48090
                     /gene="ebgA"
                     /locus_tag="ACHJZ2_001394"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO4567540.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            48087..48536
                     /gene="ebgC"
                     /locus_tag="ACHJZ2_001395"
     CDS             48087..48536
                     /gene="ebgC"
                     /locus_tag="ACHJZ2_001395"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO4567541.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVDEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            48599..>50026
                     /gene="ygjI"
                     /locus_tag="ACHJZ2_001396"
     CDS             48599..>50026
                     /gene="ygjI"
                     /locus_tag="ACHJZ2_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4567542.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNV"
BASE COUNT        12279 a        13452 c        12545 g        11750 t
ORIGIN      
        1 aatcattaaa tattaataaa ttaccatcaa ttgaattgca ttaacctctc ataaaaacac
       61 atgtagcaat tttttattat ttctcattta ctactacatt accgcatgat tgaatgattc
      121 tcaattacat atccgatggt aaaataacaa acggatagtg cttagtgagc aacaagaaac
      181 aattttcact cacgacaatc cgtgagtttt tgggataaca gactaaatgg ctaagttcac
      241 accttcattc tcaggaatca aaggtcgggc gctcttttca ctgctctttg cagcaccaat
      301 gattcatgca accgacactg caacgaccaa agatggcgaa acaatcactg ttacagcgga
      361 tgcaaatacc gcaactgagg cgaccgatgg ttatcaacct ctgagcactt ccacggcgac
      421 attaaccgat atgccgatgc tggatatccc gcaggtggtc aatacggtta gcgatcaggt
      481 tctggaaaat cagaatgcaa cgacgctgga tgaagcgctt tataacgtca gtaacgtggt
      541 acagaccaat acattaggcg gaactcagga cgcctttgta cgtcgtggtt ttggtgctaa
      601 ccgggatggc tccatcatga ccaacggcct gcgaactgta cttcctcgca gtttcaacgc
      661 cgccacagaa cgtgtggaag tgctaaaagg tccggcctcc acgctgtatg gcattctcga
      721 tcctggtgga ttgattaacg tcgtgaccaa gcgcccggaa aaaacattcc atggttctgt
      781 ctcagccacg tcctccagtt ttggcggagg cactgggcaa cttgatatca caggtcccat
      841 tgaaggcact cagctggcat accgcctgac gggggaagtg caggatgaag attactggcg
      901 aaacttcggt aaagagcgca gtacatttat tgccccgtca ctcacctggt ttggtgataa
      961 tgcaacagta accatgctct attcccatcg ggactataaa actccgttcg atcgtggaac
     1021 gattttcgac cttacgacga aacagcccgt aaacgttgat cgaaaaatac gttttgacga
     1081 accgtttaat attacagatg gtcagtccga tctggcgcaa ctcaacgcag aatatcatct
     1141 caatagccag tggacagcgc gctttgatta cagctacagc caggataaat acagcgacaa
     1201 tcaggctcgc gttaccgcgt atgatgcaac gacaggaacg ctgacacggc gtgttgatgc
     1261 aactcaggga tctacccagc gtatgcattc tactcgtgcg gatctgcaag ggaatgttga
     1321 tattgctggg ttctataatg agattctggg tggggtgtca tatgaatatt atgatcttct
     1381 gcgcacagat atgattcgct gtaaaaacgc taaagatttc aatatctaca accccgttta
     1441 tggcaatacc agcaaatgca caacggtttc ggcgtcggac agtgatcaga cgatcaaaca
     1501 ggagagctac tcagcttatg cacaggatgc gctctatctg accgataact ggattgccgt
     1561 cgccgggatc cgctatcagt attacacgca gtatgcgggt aaaggccgtc cttttaatgt
     1621 caatactgac agccgcgatg aacaatggac gcccaaactg gggttagtct acaaactgac
     1681 gccatcggta tccttatttg ctaattattc gcaaacattt atgccgcaat cgtcaattgc
     1741 cagctacatc ggagatcttc caccggaatc atctaatgct tacgaagtcg gggcaaaatt
     1801 cgagctgttc gatggtataa ccgcagatat tgcgctgttt gatatccata aacgtaacgt
     1861 gttgtatacc gaaagtattg gtgatgaaac catcgccaaa acggcaggcc gcgttcgttc
     1921 aagaggggta gaagtcgacc ttgcgggagc attaactgaa aacattaata tcattgccag
     1981 ctacggctat accgatgcta aggttctgga agatcctgat tatgcaggga aaccattgcc
     2041 gaatgttcct catcataccg gttcgctatt cctgacctat gacattcata acatgccagg
     2101 caataacaca ctgacgtttg gcggtggtgg acatggcgta agccgtcgtt cggcaaccaa
     2161 tggggctgac tattatctgc caggctattt cgttgccgat gccttcgccg catacaaaat
     2221 gaaattgcag tatccggtca cactgcaatt aaacgtcaaa aacctgtttg ataaaacgta
     2281 ttacacctct tccatcgcca caaataatct ggggaatcag attggcgatc cgcgtgaagt
     2341 gcaattcacg gtgaaaatgg aattttgatc aaaaaagccc ggtatacact tgtataccgg
     2401 gctatgtttt attcttctgg cgggaggatt atcagacctc aatctccgcc atgtcgcctt
     2461 tctcttgcaa ccagttgcgg cgatcttccg agcgtttctt cgccagcagc atatccatca
     2521 tcgcgtcagt acgctgatcg tcttcatcat cgatagtcag ctgcaccaga cggcgagtgt
     2581 tcggatcaag cgtggtttcg cgcaattgca tcgggttcat ttcccccaga cctttaaaac
     2641 gctggacgtt cggcttgcct ttcttgcgtt ttaattgctc aagtacgccc tctttctctt
     2701 cttccgtcag cgcgtaataa acctctttcc cgagatcaat acggtagagc ggtggcagtg
     2761 cgacgtaaac gtgaccgtgt ttcaccaacg cgcggaagtg ttttacgaac aaagcgcaga
     2821 gcagtgtagc aatgtgcaaa ccatcagagt ccgcatccgc gaggatacag attttgccat
     2881 aacgaagctg gctcagatcg tcgctgtcag gatcgatacc gatcgctacc gaaatatcat
     2941 gcacttcctg cgaggccagc acttcgtcgg aagaaacttc ccaggtgtta aggatcttac
     3001 ctttcagcgg catgatcgcc tgatattcgc gatcgcgcgc ctgcttggca gatccgcctg
     3061 cggagtcacc ttccacgagg aacagttcgg tacggttaag gtcctgcgcg gtacaatcag
     3121 ccagtttgcc aggcagcgcc gggccgctgg tcagcttttt acgcaccact tttttagccg
     3181 cacgcatacg acgctgggcg ctggaaatcg ccatctctgc cagcaactcc gccgcctgaa
     3241 cgttctggtt cagccacagg atgaaggcat ctttcaccac gcccgaaacg aatgccgcac
     3301 actgacgcga agagagacgc tctttggtct gcccggcaaa ctgtggatcc tgcattttta
     3361 ctgacagcac ataggcgcag cgatcccaga tatcttccgc cgacaacttt acaccgcgcg
     3421 gcaaaatgtt gcggtattca cagaactcac gcatcgcgtc cagcaggccc tgacgcaggc
     3481 cattaacatg ggtaccgccc tgcatcgttg ggatcaggtt gacgtagctt tcggtcagca
     3541 gttcaccgcc ttccggcagc cacagtagcg cccagtccac cgcttcagta tcgccagcga
     3601 aattaccgat aaacggtttt tctggcagcg tcggtaaacc gtttaccgct tccgccaggt
     3661 aatcattcag accgtcctga tagcaccagc gctgttcggt gttgttgatc tcatctttaa
     3721 aggtaatctc aacgccgggg cacagtactg ctttggcttt cagcacatgc gtcaggcgtg
     3781 aaacagaaaa acgcgggcta tcgaagaagg tttcatccgg ccagaaatgg acgctggtgc
     3841 cggtattgcg tttaccgcaa ttgccgacaa cctgtaaatc ctgcaccttt tcgccatttt
     3901 caaaggcgat gttataaacc tgaccatcgc ggcgcacgtt aacttctacg cgcttcgaca
     3961 gggcgttaac caccgaaatc cccacgccat gcaggccgcc agagaactgg taatttttgt
     4021 tagagaattt accgcctgca tgcagacggc aaagaatcag ttcaaccgcc ggtacaccct
     4081 cttccgggtg aatatccacc ggcatcccgc gcccatcgtc aataacttct aacgactggt
     4141 cagcatgtaa aataacgtcc acgcgttttg cgtgacccgc cagtgcttca tccacactgt
     4201 tatcaatgac ttcttgcccc aaatggttag ggcgagtggt atcggtatac atccccggac
     4261 ggcggcgaac cggctcaagc ccggtgagta cctcaatggc atcagcgtta taagtttgcg
     4321 tcatggttta agttagcaat tcgagttgat cgtcagagat ggtgcagacc aagaaaatcg
     4381 acgatcgggt tgaaataatc ttcgaagccc gtgaatgcgt ggttgccgcc ttctatgaca
     4441 gtctggcggc aggaagcgta gtacgccacc gcctggcggt aatccagcac ttcatctccc
     4501 gtctgttgca gcagccagat caaatccggc gcttccagcg ggtcaatctg catgacttta
     4561 agatcgtaaa tatggcgtga ctctagcaca tattgctgcc cggtgtaggg gttctcgttc
     4621 tgaccgagat agtccgtcag cagttcaaac gggcgcaccg ccgggtttac caccactgcg
     4681 ggcagcataa aacattgtga caaccaggtg gcgtaatatc cccccagtga cgaaccgaca
     4741 atacccagcg aatcgccgcc atgttccagg acaatggatt ccagcagctc tgccgcgtcg
     4801 gaaggatacg gcggcaactg cggaatgatc atctcaacgt cagggtgatg ttccgccagc
     4861 cagtttttta acaagctcgc ttttgcagag cgcggcgagc tgttgaaacc gtgtaaataa
     4921 agaagcgtag acatcagtag ccttctgaag cggtatcagg ttggaaacgt gtgtccgcca
     4981 ggcgatgcac ctcggtggtc agcgtgccat cagcatgtaa ctcgagagta cgccagccgg
     5041 gcgcgatagt atccagcgta aagttggaac agtgcggctt aaactgcaca caggtcgacg
     5101 gtgttgccag caggcggcga ccgttccagt cgagatccag ctcctgatga atatgaccgc
     5161 acagcaagta tttgacgtgc ggaaacttcg ccagcacggt atccagttcg cccgcgttac
     5221 gcaggctgtg ttgatcaagc caactacaac ccgcaggtag cggatgatga tgcagcagca
     5281 gcaacgtatg gcgctctggc gcatcagcca gtttacgttc cagccactca agctgaaact
     5341 cgcttagctc accgtgcggc acgccaaaca cctggctatc cagcaacagg atttgccatt
     5401 gctcaccaat aaacacccgc ttcgccgggg agatacccgc atcctgtaac gcgctgtaca
     5461 tcgcgggctg gaaatcgtgg ttgcccggca gccagacgca gggcgcacga aaacttgcga
     5521 tgccttcagc gaaatgctga taggccgcag aggattgatc ctgcgctaaa tcacctgtcg
     5581 cgacaatcag gtcgaattcg tgctggtgtg gacgaatcgc ctccagcacc gcctggtagc
     5641 tttcccaggt gtttacccct aagagggctt cgtgcttttg tgcaaacagg tgagtatcgg
     5701 taatttgtaa aatcctgact ctggcctcac cagccagagg aagggttaac aggctttcca
     5761 aatggtgtcc ttaggtttca cgacgctaat aaaccggaat cgccatcgct ccatgtgcta
     5821 aacagtatcg caaccagtcc gctaaaaact ggttaatttg atgcttttcg tcgcgttgat
     5881 gcaacttttt attaggataa tcataccgcg ctttgaagcg aaaaatctgc tggcttgaac
     5941 acacttcagc caccatcgcg tcatgataca gacgcaccgt cattgacgga aggctccagt
     6001 aactgatcgc gtgcgcagtc tgttctattg tcaccagggt agtgtatcgg gtcgattcca
     6061 caatcgtcag ccgatattgt gcgtttgcca cctgatagct tacagtttcg ccgggtgcgt
     6121 cattgcgcgg taacaaacgg cgcaattgtg aaaagttcat ctcgcacagg cgcatcattt
     6181 caggaaagtc aggtgtgtaa cgcttcattt atgcccactc attttttaac gcttgatgat
     6241 gcagctgcag ccattgcaaa gcgatgaccg acgctgcgtt gtcgattttc ccctcttcta
     6301 cccactggta tgcctgttcc cggcttacca catgaacgcg aatatcttcg ttttcatcag
     6361 ccagaccgtg aataccgctt gcggtcgtgg cgtccacttc gcccaccata attgacgaac
     6421 gctcactggt gccccccggg cttgccagga aacttaacac cggtttggtc cgtttgacta
     6481 tcagccccgc ctcttcaatc gcttcgcgac gggcaacatc ttccacactt tcaccctctt
     6541 caatcattcc ggcaaccatc tccagtagcc aaggggtttc gctagtgtcg tatgcggcaa
     6601 ttcgaatctg ctcaatcagc acaacttcat cacgcactgg gtcaaagggt agcaagactg
     6661 cggcgtgacc gcgctcaaaa atttcccgcc gtacctcatg actcatttgc ccgttgaata
     6721 gacgatgacg aaatctataa agatctaatg aaaaaaagcc gcgataaagt gtttctcgtg
     6781 caataatttc tacatcgttt ttgccaaatg taacgggcag gttgtctggc ttaagcattg
     6841 ttaatgtcct ggcactaata gtgaattaaa tgtgaatttc agcgacgttt gactgccgtt
     6901 tgagcagtca tgtgttaaat tgaggcacat taacgcccta tggcacgtaa cgccaacctt
     6961 ttgcggtagc ggcttctgct agaatccgca ataattttac agtttgatcg cgctaaatac
     7021 tgcttcacca caaggaatgc aaatgaagaa attgctcccc attcttatcg gcctgagcct
     7081 ttctgggttc agttcgttga gccaggccga gaacctgatg caagtttatc agcaagcacg
     7141 ccttagtaac ccggaattgc gtaagtctgc cgccgatcgt gatgctgcct ttgaaaaaat
     7201 taatgaagcg cgcagtccat tactgccaca gctaggttta ggtgcagatt acacctatag
     7261 caacggctac cgcgacgcga acggcatcaa ctctaacgcg accagtgcgt ccctgcagtt
     7321 aactcaatcc atttttgata tgtcgaaatg gcgtgcgtta acgctgcagg aaaaagcagc
     7381 agggattcag gacgtcacgt atcagaccga tcagcaaacc ttgatcctca acaccgcgac
     7441 cgcttatttc aacgtgttga atgctattga cgttctttcc tatacacagg cgcaaaaaga
     7501 agcgatctac cgtcaattag atcaaaccac ccaacgtttt aacgtgggcc tggtagcgat
     7561 caccgacgtg cagaacgccc gcgcgcagta cgataccgtg ctggcgaacg aagtgaccgc
     7621 acgtaataac cttgataacg cggtagagca gctgcgccag atcaccggta actactatcc
     7681 ggaactggcg gcgctgaatg tcgaaaactt taaaaccgac aaaccacagc cggttaacgc
     7741 gctgctgaaa gaagccgaaa aacgcaacct gtcgctgtta caggcacgct tgagccagga
     7801 cctggcgcgc gagcaaattc gccaggcgca ggatggtcac ttaccgactc tggatttaac
     7861 ggcttctagc gggatttctg acacctctta tagcggttcg aaaacccgtg gtgccgctgg
     7921 tacccagtat gacgatagca atatgggcca gaacaaagtt ggcctgagct tctcgctgcc
     7981 gatttatcag ggcggaatgg ttaactcgca ggtgaaacag gcacagtaca actttgttgg
     8041 tgccagcgag caactggaaa gcgcgcatcg tagcgtcgtg cagaccgtac gttcctcttt
     8101 caacaacatt aatgcttcta tcagtagtat taacgcctac aaacaagccg tagtttccgc
     8161 tcaaagctca ttagacgcga tggaagcggg ctactcggtc ggtacgcgta ccattgttga
     8221 tgtgttggat gcgaccacca cgctgtacaa cgccaagcaa gagctggcga atgcgcgtta
     8281 taactacctg attaatcagc tgaatattaa gtcagccctg ggtacgttga acgagcagga
     8341 tctgctggca ctgaacaatg cgctgagcaa accggtttcc actaatccgg aaaacgttgc
     8401 cccgcaaacg ccggaacaga atgctattgc tgatggttat gcgcctgata gcccggcacc
     8461 cgtcgttcag caaacatccg cacgcactac caccagtaac ggtcataacc ctttccgtaa
     8521 ctgatgacga cgacggggct tcggccccgt ctgaacgtaa ggcaacgtaa agatacgggt
     8581 tatctgccgc attcttcccc cttctcgctt caatttcgac cagccatcct ctattctgat
     8641 gggtatttac cacaggtccc ggaagacaaa aatgaaacgg acaaaatcca tacgccacgc
     8701 atcgttccgc aaaaactgga gcgcacgcca tctgacacca gtcgctctcg cggttgccac
     8761 tgtttttatg ctggctggct gtgaaaagag tgatgaaaca gtgtctctct atcaaaatgc
     8821 tgacgactgt tcagctgcaa acccaggcaa aagcgccgaa tgtaccaccg cgtacaacaa
     8881 tgcgctgaaa gaagccgaac gtactgcgcc gaaatacgcc acccgtgaag actgtgttgc
     8941 tgaatttggt gaaggtcagt gccagcaggc accagcccag gctggcatgg caccagaaaa
     9001 ccaggcgcag gcccagcaat ccagcgggag tttctggatg ccgctgatgg ccggttacat
     9061 gatgggtcgt ctgatgggcg gcggtgcggg atttgcacag cagccgctgt tctcctcgaa
     9121 aaacccagcc agcccggctt acggtaaata taccgacgcg acgggtaaaa actatggcgc
     9181 agcccagcca ggccgcacca tgaccgtacc gaagacagcc atggcaccaa aaccggcgac
     9241 caccactacc gttacccgtg gcggttttgg tgaatctgtt gccaaacaaa gcactatgca
     9301 gcgtagcgca accggtacct cttctcgttc aatgggtggc tgataccgat ggaaagagtc
     9361 agtattaccg agcgcccgga ctggcgtgag aaagcccacg aatacggttt caattttcac
     9421 accatgtacg gcgagccgta ctggtgtgaa gatgcttact acaagttgac cctcgcccag
     9481 gttgaaaagc tggaagaagt caccgccgaa ctgcaccaga tgtgcctgaa agtggtggaa
     9541 aaagtgatcg ccagcgatga gctgatgacc aaattccgca ttccaaaaca cacctggagt
     9601 tttgtgcgcc agtcatggct gacgcaccag ccatcgcttt attcgcgtct tgatctggcg
     9661 tgggatggca ctggcgaacc taaacttctg gaaaataacg ccgatacgcc aacgtcacta
     9721 tacgaggcgg cgttctttca gtggatctgg ctggaagatc agcttaacgc cggtaacttg
     9781 ccggagggca gcgaccagtt taacagtctg caagaaaaac tgatcgatcg cttcgttgag
     9841 ctgcgtgaac agtatggctt ccagttgctg catctcacct gctgtcgcga cacggtggaa
     9901 gatcgcggta ccattcagta tttgcaggac tgcgcgacgg aagctgaaat cgctaccgag
     9961 ttcctctaca ttgacgatat cgggttaggt gaaaaaggtc agttcacgga tttacaggat
    10021 caggtaattt ccaacctgtt caagctgtat ccgtgggaat ttatgttgcg tgagatgttt
    10081 tccaccaagc tggaggatgc aggcgtacgc tggctggaac cggcgtggaa gagcattatc
    10141 tccaacaagg cacttctacc gctactgtgg gagatgttcc cgaatcaccc gaacctgctg
    10201 cccgcttatt ttgcggaaga tgatcatccg caaatggaaa aatatgtggt taaaccgatc
    10261 ttctcccgtg aaggcgcaaa cgtgtcgatc attgagaacg gcaaaaccat tgaagcagcg
    10321 gaaggtccgt atggcgaaga agggatgatt gttcagcaat tccacccgtt accgaaattc
    10381 ggcgacagct atatgctgat tggtagctgg ctggtgaacg atcaacccgc cggaattggc
    10441 attcgtgaag accgtgcatt gatcacccag gatatgtctc ggttttatcc acatattttt
    10501 gttgaataat caatgatgcc ggatggcatt cgccatccgg taattgttaa cctatctgca
    10561 ccgacagcat acttaaactg cccatttcta taccatcgac cggaatggta attggctcct
    10621 gcccatccca cgcacctaag acatacagca acggcagata gtgctctggc gttgggttcg
    10681 ataacgcgcc accttcatgg tcgagataat tcaccagagg atgctgctct accggccctt
    10741 gccacgtcag attcgctttc acatactcat taaacgacat cgcccaagga tacggtgaac
    10801 tatcaccgtg ccacttcact gtacgcaggt tatgcaccac gttgccgctg gcgaccaaca
    10861 ttattccttc atcacgcagc gccgcgagtt ttcgccccat ttcgaaatgc caggcggcag
    10921 gtttgctact gtcgatactc aactgcacca tcgggatatc cgcgtcagga tacatcttaa
    10981 tcagcacgcc ccaggaaccg tggtcaaagc cccaggcttc tttatccagc gtcaccggga
    11041 tcggcgctaa cagctcaacc agacgctgtg ccagcgcagg cgaacccgga gcaggataat
    11101 gcgtatcgta cagcgcctgc gggaagccac caaagtcatg aatcgtgggc ggcgtctcca
    11161 tcgcggtcac tcctgttcca cgggtaaacc agtgagccga aactaccaca atcgcttgcg
    11221 ggcgtggcaa tgtcatcccc aacgtctgcc agctgcgggt atacaaatta tcttccagca
    11281 cgttcatcgg actaccgtga cctaaaaaca atgctggcat acgtgttgaa gacatgatga
    11341 tatccttaac taaaggtgtc attttgatat cctcacgata cgcttgttcg gcggagtaag
    11401 aacccggata acaatgatga tgatcatcag ttattttgac gatctgcctg aaggtgaaga
    11461 tttataagga gttgtcgatg tcagtacctc tcattctgac catactggcg ggggcagcca
    11521 cgtttattgg cgcgtttctc ggcgttctcg ggcaaaaacc ctcgaaccgc ttactggcgt
    11581 tttcgctagg atttgcggcg gggatcatgt tgctcatctc attaatggaa atgcttcctg
    11641 ccgcactagc agctgaagga atgtcgcctg tgttgggtta tggaatgttt atcttcggtc
    11701 tgcttggcta ttttggcctg gaccgcatgt tgccgcatgc gcatccgcag gatttaatgc
    11761 aaaaatcggt gcagccgttg ccaaaatcga tcaagcgcac agccattctg ctcactctcg
    11821 gcatcagtct gcataacttc ccggaaggga ttgccacctt tgtcacggcg agcagcaacc
    11881 tggagctggg atttggcatc gcactggccg tcgcgttgca caatattcct gaaggtctgg
    11941 cggttgcagg cccggtttat gcagcaacgg gttctaaacg cacggcaatt ctgtgggcgg
    12001 ggatttctgg actggcagaa attcttggtg gtgtgctggc gtggttaatc ctcggtagca
    12061 tgatttcccc ggtggtcatg gcagcaatca tggcggcggt cgcaggaatt atggtggcgc
    12121 tctcggttga tgaattaatg ccgctcgcca aagagattga ccctaataat aaccccagct
    12181 atggcgtact atgtggaatg tcagtgatgg gattcagttt ggtgctgcta caaacggcgg
    12241 gaattggtta aaagcaacat cagaagttgt ccagcgcagg gacaacttct gctagtttca
    12301 ctaaagcctt attctacctt ttcggctgtc tcttctctcg tactgtttaa gtatttgttc
    12361 cgcgcttcaa gcagtgcgtc tctgtcctgg cgaaatgccc gacaataacg tttcaaatgc
    12421 aggaaataac ccaccacaac cagaacaatc aatacaatcc agtaccaggc gataaacatc
    12481 cttttaccct taatatttaa caatactaaa catttagcgc ataaatttca catatccttt
    12541 ttcggatata ttcatatggt cgcataataa cggaacaatt atatgatttt gatcataatg
    12601 aaaaagaatg cctgatatta atgaaatttt ctcatagaaa gagaagcgga attaacatct
    12661 tgcatagtag ggaaaaataa aaaccgaagc ctgctacaac ttcggtttct tgattaaggc
    12721 agtaaattaa gcagcggttt tcagctggct ttacgctcat gtgcctgacg gtatgccacc
    12781 aggtcttcaa tagtcacgag cgccatgttg tgtttattgg caaactcaat gcactctggt
    12841 gcacgcgcca tcgtgccatc gtcattagtc agctcacaca gcacaccagc cggtttgaag
    12901 cctgccagcg tcattagatc aatagttgct tcagtatgac cgccacgcgt cagtacacca
    12961 ccagcctgag cgcggagtgg gaaaacgtgg cccgggcgat tcagatcaga aggttttgcg
    13021 ccatcggcaa tcgctgcgcg aacggtcgta atacggtcag cggcagaaac accggtagtc
    13081 acaccttcag ccgcttcaat ggtcacggta aaaccggtgc cataggcgct ggtgttattt
    13141 tctaccatca ttggcagatc gagttgttta cggcgatctt cagtaatgca caggcaaaca
    13201 ataccactac cgtggcgaat ggtcagcgcc atctgctcaa cagtcatggt ttctgccggg
    13261 aagatcatat caccttcgtt ttcacggtct tcatcatcaa gcaccattac accgcgtcct
    13321 tcacgcagcg cagccagtgc attttcaaca cgttcgaaag gcgtaccaaa agaggaaagt
    13381 agcgtctgat tcatggtaaa aaaacctcac taaaattatg gttaccagaa tcagggcagt
    13441 cttaggagtg gcggcatata gccaaaataa cgtgagcggg tccatgcccg acagaatcgt
    13501 tactctctcc catccggact ctaaccgtcg gccccggaat tacaccggat ctgctgtcct
    13561 ttgagttcgc acccaaagcg ctcgcgggct ttcaactgag ttgatttacc gccggtgggg
    13621 aatttcgccc cgccctgaga ataagcggat tcactataac gctaatgatt agcggcagca
    13681 acgcatagct tcacataatt ctggtttatg acttaccctt atcgcactac aatggcactc
    13741 aacacctatc agtacaggga aactacaatg attgacccga aaaaaattga gcaaatcgct
    13801 cgccaggttc acgaatcaat gcctaaagga atcagggagt tcggggaaga tgtggagaaa
    13861 aaaatccgcc aaaccctaca agcgcagctg acgcgtctcg atctggtaag ccgcgaagaa
    13921 ttcgacgttc agacgcaagt cctgttgcgg actcgtgaaa aactggcgtt actggagcag
    13981 cgcagcagtg aactggaagc gcgcaataat tcggtagcag atcttcaatc tcctccggct
    14041 atcccgccta ttgataaagc agaataaagc aacacaaaaa cgggccagac ggcccgtaat
    14101 atcagtgttc taattcaagt tctctggttt gcatcatctg ttggcaatac caggcataac
    14161 gtgcgcaaaa ccaggttcta tgttcttcat ccatgttacc agtaacggcc agaatatcga
    14221 ccgggcgacc ttctgcgtgc attctggcaa aactacgcgc taggaaatcg aaattattta
    14281 aagaattaag actatgatcc ataagcacat cctccggtta tcgcatacct tttaaggcca
    14341 tatgcttttt ctgatttcag tattccccaa tcagtgtagt gcctttaaac ataacgtgcg
    14401 taaatatatt gtccccgatc acacttttta gtgaaaaata aaaaaccata ttaaaagact
    14461 ttctcttcca gaacctctct aacatatgtt attattaaca aagacaaatg taagcaagga
    14521 aactatcaca aagtaaatat tgcgtcacta aatggacatt ggagtgacat atgattttat
    14581 tcgccgacta taacacccct tatcttttcg ctatttcctt tgtactgttg atcggccttt
    14641 tagaaatttt tgcgcttatc tgcggtcaca tgctctccgg tgctcttgat gcacatcttg
    14701 accattatga ttccataacc acaggtcata ttagccaggc acttcattat ctcaatatcg
    14761 gaagattgcc agctctcgtc gttctttgct tactggctgg tttttttggt cttattggca
    14821 tcctgttgca gcatacctgc atcatggtct ggcaatcacc actctcgaac ctgtttgtgg
    14881 ttcctgtcag tttactattt acgattattg cggtgcatta taccggtaaa atagttgcgc
    14941 cctggattcc acgcgatcat agctcagcta ttacagaaga agaatatatt ggtagcatgg
    15001 cgttaattac cggtcatcaa gccacttcag gtaatccctg cgaaggaaaa ctcaccgatc
    15061 attttggcca aatacattat ttactactgg agcctgaaga aggaaaaatt ttcactaaag
    15121 gagacaaagt gttaattatt tgccgacttt ctgcaacgcg atatttggcg gaaaacaatc
    15181 cctagcccca aatattgtaa tttattaatc aaaaggaaat ttaataatgg atgatattgt
    15241 taattccgtg ccctcctgga tgtttaccgc gattattgcc gtatgcattc tgtttattat
    15301 tggaattatt ttcgccaggc tctatcgtcg cgcttcggca gagcaagcat ttgtccgtac
    15361 cggcttaggg gggcaaaaag tggtaatgag cggtggcgca atcgtgatgc cgatctttca
    15421 tgaaataatc cccatcaata tgaatactct gaagctggaa gtcagccgct caaccattga
    15481 tagcctgatt acgaaagatc gtatgcgcgt cgatgtagta gtcgctttct ttgtgcgggt
    15541 aaaaccttca gtagaaggga ttgccaccgc tgcccagacg ctggggcaac gcaccctgtc
    15601 gcctgaagac ttacgtatgt tggttgaaga taaatttgtc gatgccctcc gtgcaacagc
    15661 tgcgcaaatg accatgcatg agttacagga tacccgcgag aacttcgtgc agggagtaca
    15721 aaataccgta gctgaagatc tgtcgaaaaa cggcctggaa ctggaaagcg tttcacttac
    15781 caactttaac cagacctcga aagaacattt caatcctaac aatgcctttg acgccgaagg
    15841 tttaaccaaa ctgactcagg agacggagcg ccgtcgccgc gagcgtaacg aagttgaaca
    15901 ggatgtagaa gttgcggtgc gtgagaaaaa ccgtgatgca ctttcgcgca agttggagat
    15961 tgaacaacaa gaagcgttta tgacgcttga gcaggagcag caggttaaaa cccgtaccgt
    16021 tgagcagaat gcgaaaattg cggcttttga agctgaacgt cgtcgtgaag cagagcagac
    16081 gcgaattctg gctgaacgac agattcagga aacagaaatc gaccgcgaac aggccgtccg
    16141 ctcaagaaaa gttgaagctg aacgtgaagt tcgcattaaa gagatcgaac agcagcaggt
    16201 caccgaaatc gctaaccaga cgaaatcgat cgctattgcc gccaaatcgg aacaacagtc
    16261 ccaggcagaa gcgcgtgcta accttgcact cgcagaagca gtaagcgccc aacaaaacgt
    16321 agaaaccact cgccagacag ccgaagccga tcgtgccaag caggtggcgc tgattgccgc
    16381 agcacaggat gcagaaacca aagcggttga actgaccgtg cgggcgaaag cagaaaaaga
    16441 agccgcagag atgcaggcgg cggcgatcgt tgagttagcc gaagctacgc gtaaaaaagg
    16501 cctggcggaa gcagaagcac aacgtgcgct gaacgatgct atcaacgtac tttctgacga
    16561 acaaaccagc cttaaattca aactggcctt gttgcaggcg ctacctgcgg taatagagaa
    16621 atccgtggaa ccaatgaagt ccattgacgg tatcaagatt attcaggtcg atggtctgaa
    16681 tcgtggtggt actgcggggg atgcaaacac gggtaatgtg gggggcggaa acctggcgga
    16741 acaagcatta tcagccgcac tctcttaccg cacacaggca ccactgattg actccttgct
    16801 caatgaaatt ggcgtttcag gcggctcact ggctgcattg acttcactct taaccccaac
    16861 aactcccgtc gccgaaaacg tagaataaat tttcagctat gaacatccct ggctcaaaaa
    16921 gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgatcagg ctgttgcagc
    16981 gttttgcgtg attgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg cgcggggtcc
    17041 ggcgctcatc aacaatcggg gagtaagcaa agggctgaaa cgggaaagcc cctcccgagg
    17101 aaggggcctt acagaaggaa agggttatga tgaagctcgt catcatactg attgtgttgt
    17161 tactcgtaag tttcgcagct tattaacagc caatcagagg ggggagaaat cctccctcac
    17221 ccttgttcct ttactttagg ttgaaaaaaa atcagcgtca agaaggtttc attaacacat
    17281 atccctggcc caaaaagccg gggatttttt atatctgcgt tccgctaaaa ggtgcaaatg
    17341 ctcaggacgt tgcagcgttt tgcgtgaccg ctcggggaag gcaaaactgc ttcagggaaa
    17401 gcgttgcgcg gggtccggcg ctcatcaaca atcggggagt cagcaagggg ctgaaacggg
    17461 aaagcccctc ccgaggaagg ggccttatag aaggaaaggg ttatgatgaa gctcgtcatc
    17521 atactggttg tgttgttact gttaagtttc ccgacttact aacaactcat cagagggggg
    17581 agaaatcctc ccttaccctt gttcctttac tctaggttga aaagacaaca gcgtcaatag
    17641 gcctgccatg tacgaagcga gatctgtgaa ccgctttccg gttagccttt tttatcctgt
    17701 tggatcttct tgataatgtt agtggtcgag caaccgtctt caaagttgag caccaacact
    17761 tcgccgccgt tggcccagac ttctttactc ccggcgatct cttccggttt gtagtcaccg
    17821 cctttcacca gcaggtctgg caggatcccg gcgatcagac gctgcggcgt gtcttcttca
    17881 aacgacacta cccagtcgac cgcttccagt gcgcccagca caatcatacg ctgttcgagt
    17941 gggtttaccg ggcgggaatc ccctttcagc cgtttggtgg aggcatcgct gttgacggca
    18001 acaatcaagc ggtcacccag cttgcgggca tttgccagat aagagacgtg cccggcatgt
    18061 aggatatcga agacaccgtt ggtcattacc actttttcac cacgtttacg cgctgccact
    18121 acagccagct tcagttcctc ttcggtcatc acgccaaagc cggtatctgc gcgtccacgt
    18181 acagcgtttt ccagctcgat cggcgaaacc gtggacgtcc ccagtttgcc gaccaccaca
    18241 ccagccgccg cattggcaaa gaagcaggct tcttccagcg aattacccgc tgccagcgtt
    18301 gccgccagga cgccaatcac cgtgtcgccc gcaccggtaa cgtcatacac ttcctgcgcc
    18361 tgagttggca tatgcagcgg cgctttaccc ggttgcagca gcgacatacc ctgttcggaa
    18421 cgggtcacta acagagccga gagttcgtaa tcggcaatca gtttcatgcc gcgctcaaca
    18481 atctcttctt cggtcttaca tttaccgaca acagcttcaa attccgagag attcggcgtt
    18541 aacagcgtag cgccgcggta gcgctcaaaa tcggtacctt ttggatcaat cagcaccgga
    18601 acacccgctt tacgcgccag ttggatcatc tgctgtacgc ttgccagcgc acctttggcg
    18661 tagtcagaaa gcaccagcgc gccaatcgaa ctcagcgcct gattaatccg ctcgtgcagc
    18721 ggctgcggat caacaccttc gaaaccttct tcaaaatcca gacggatcag ctgttggttg
    18781 cgggaaagta cccgtaattt ggtaatggtc ggatgcgtcg gtacagaaac gaagtcgcat
    18841 ttgacgttga cgtcggccag agatttactc agcgcgcgcg ctgcatcgtc aatgcccgtc
    18901 aacccgacca ggcgtgcatt agcaccgaga gaagcgatat tcatcgccac gttagccgcg
    18961 ccgcccggac gttcttcgat ggtattcact ttaaccacgg gcaccggcgc ttccggcgag
    19021 atacgactgg aggggccgta ccagtaacga tccagcatca catcaccaac caccatcact
    19081 cctgcacgtt caaactctgg cagcgttact ttcattcctg tctcctgaga gattcaaaat
    19141 ttgcgcgcga taataccata cttcattctt ccaccagcca cttctgccag cttgcccgca
    19201 ccagttcacg ctctgcggtg aagcaatcct ccggcacatg gcccggcaat tcctgtaatg
    19261 ccagatgatg aagttcatcg cgaagcgtag tgtaagcacg ggtcagcgcc atcgcttcct
    19321 gctcttccat aatgtcgttt tgcgccagta gttccagaat gcgcacgttg tctgaccagc
    19381 gcgttaactt cggtttttca tgagcgtagc gcaacaccag atattgggta ataaattcga
    19441 tatcggtaat tcccccttca tcagctttga tatcaaagcg atcgcgatgt ttattgccga
    19501 gatgagcgcg cattttctcg cgcatttccc gcacttccgt ttgcagagtt ttaccttcac
    19561 gcggcagcgt cataatctcg cgacgcactg cgtcaaagtg cgcggtgagc tgcggatcgc
    19621 cgtacactac acgcgcacgc accagcgcct gatgctccca cgtccaggcc tcgtttttct
    19681 gataatcggc aaatgcttct gtggaggtaa ccagcattcc cgccgccccg gacgggcgca
    19741 gccgcgcatc cacttcgtac aaaatgccgg aagaggttcg cgtgctgaac aggtgcataa
    19801 tgcgttgcgc cagacgcaga taaaactgtc gtccgtcgat ttcccgctcg ccgtcagtca
    19861 tcgcatccat tgggcaatca tggaggaaga tcaggtcaag atcggaactg tagcctaact
    19921 cccagccgcc cagcttgccg tagccgacca ctgcaaaacc acgcccttcg cgttcgttca
    19981 ggtgattcgg cttaccgtag cgggcaacca tttgaaccca cgcctgctga acgacggcat
    20041 ctatcatggc ttccgccagc caggttaagt gatcgctcac tttcatcacc ggtagcgtac
    20101 cggcgatatc cgctgcggcg atgcgtaaca gctgcgcctg tttgaactga cgcagcgcct
    20161 caagctgttg ctcttcgtca tcttccggca cgcgcagcaa atactggcgc aactcatcgc
    20221 ggtaggcatc ggtcgccgtc ggctggtaaa gggtgtttgg atcgagcaat tcatccagca
    20281 ataatggata acgcgccagc tggctggcaa tcatcggcga cgctgcacac agagaaatca
    20341 aatgtttaag cgccgcgggg aattcactga gcaattctaa ataggtggtg cgggtaacaa
    20401 tccccgccag caaggcggta atgcgcaaca gcgtaacggc agcgtcttca cgcgcacaga
    20461 catcacttag cagatgcggc atcagatggt cgagcacctg acgcccgcgt ggcccgattg
    20521 tgcgcttatc cagctctttg cggaaatcgg caatcagcgt cagcacctgt ttgcgatcgt
    20581 cctcgctaag atgcgccagc actggtgtag tgtcatcttc ctgcaacgca tcctgccaca
    20641 gctcgcgcca ctgttccgac agcgactctt cctgagtttc actttcgtcg tcgccaatca
    20701 attcattaaa cacccggcgc acattggtca tatgtgcggt cagcgccccg gtcaattgcg
    20761 gccagtcagc aaagtccatc gcccacgcca gccgcgcacg gttaagctca tcagaaggaa
    20821 gcgtctgggt ttgttcgtcg ttaatgcttt gcagcaggtt ttccagacgc cgcaggaaca
    20881 gatacgccac tcgcaattgt tcagcatcgt tttcagaaag cagatgcagc gcggcaatga
    20941 cgctgagcgt tggcagtaaa gagcgcgact gcagcgacgg ttcgcgcccg ccgcgaatga
    21001 gctggaacac ctgaacgata aattcaattt cgcgaatgcc gcctgcgccg agtttgatat
    21061 tgtcggtcaa accacgtcga cgcacttcac gggcaatcat tcctttcatg ttgcgcagcg
    21121 actgaatcac gctgaaatcg atgtaacgac ggaaaacaaa cgggcgcagc atcgcacgca
    21181 actcgttagc atagacgcct tcgctatcgc ccataatccg cgccttgacc atcgcgtaac
    21241 gctcccagtc gcgcccctgc tcctggtaat aatcttccaa cgcggcaaag ctcagcacca
    21301 gcgggccact ttcgccaaac ggacgcagcc gcatatccac gcgatagacg aagccatcct
    21361 gcgttggttg atccagcact ttaatcagcc gctgccccat gcgggtaaaa aactgcgcgt
    21421 tatccagttc ccggcgtcca ccctgcgtac aaccatgttc cggccaggca aaaatcagat
    21481 cgatatcaga ggagaaattc agttccccgc cgcccagctt acccatgcct aaaatcagca
    21541 gcggttgcgc ttcgccctgc gcattgcacg gcgttcccca ctcgcggcag caggcgtcat
    21601 atagccagtc acgcgccgca acaatcagcg tctccgccag atagctgagc tgctgcaata
    21661 tgctctcttc agtaaccagt gccagcgttt gcgcccaggc gatacgtacc atgatgcgcc
    21721 gacggaacag ccgcagctcg cgcattaacc cggcttcgtc actcacatta atgagcgcct
    21781 cctgcaacca tgcaacgtaa tgctgccatt cgtcggcctg cggcggttgg ctttccagtt
    21841 ccgtcagcca ctctggatgc gcacttatgc tgtcctgcac aaaatcactg aaagtaagta
    21901 ctgacttcgc ctgtgcacta agtgattcct cggctaaagg ctctggcagc cgctcaacaa
    21961 tggtctgcca gtactgctgt aacggtgaag agagcggctt cataagcgat tttatccttg
    22021 cctgattaac gttttccgct gtgcaaccag aacggttcct gattgtttgc ctcattacgg
    22081 aaatgttcaa tttcgatgcg ttgcccggtc gcaatagcgt gatgcagccc ctgccagttc
    22141 tccagccagg cttgcgcgac gacaggatca tagtaacccg ccagcaacag tattgagtca
    22201 atatcacgcg tcaggcgtgg caactggtca cggtagcgat cgcctaacgg ctggcagaaa
    22261 acgcttttca gttcagcggc atggcgggaa agatggatat cggcaaagcg tttgaaggag
    22321 tcgctgattt tgccctgcgc tttggcatct aaaaatggct gccatgcttt gcttaccaac
    22381 cattcggtca acgccagctt cgccattgcc gtttcggtag agtagaccgc cgtcacggca
    22441 gaaaccgcag aagcaatggt cgcttcgcat tgggtcagca aatcacgtaa gtgagtgctc
    22501 gctttacgcg gcacaatacc accgaacagc atcagcgtat gacggaccaa gctaatggct
    22561 gccagcacct gttctttcgc cgcatcgttg ccgcgtaccc acagttcttc atgatactgc
    22621 cattgcgcta acgccagctc gagcgccgct tccagcccct gttccacatc ggcttttgcc
    22681 gcaacatgca aaatggtggt cggtttgatt tcacgcgccg gattgccctg cgccagatga
    22741 tagccacgcg ccgctttgct caggctgccc tggcgtaatc cggtttgcga taccagttgg
    22801 ttcgccagtt tcagcaccgc gcgcgtgtcg ccgctaagca gctccagttc cagctcacag
    22861 ataggttcag caaattcccc cgctttcacc tcaccctgat cgagagcgat ttcaattcgg
    22921 ctgccatcga ccgccaccag ccatttttcg cgataaaaat cggtgctgaa cagcggttgt
    22981 acgcgggaag cgagatcggc gggcaattcg ccgttcggcc agacttccgt cggtaactgc
    23041 gccaggtcga gcgtcggttc gctcaacgcc acgttatatt ccgggcgctg gtgtaagccg
    23101 cctgtcactc ttcctgcaac tttcatggtc atctcatagc gaccgttttc gccacgaata
    23161 cgtaagccca tatcgtgccc acgcagccag ttatccggcg tttcgtagta aatattcagt
    23221 aactgcacgg ggtcatggtg ctcgccgccc agcgtattga gatggtcacg caacgcctca
    23281 acggcgctgt gattaacaat aaactttaat tcgatttcct gagccatggc ctttacttat
    23341 cggttatgtc acataagtga cgatgaacgg cgaacttaat gcgatctttt ttgtcagtag
    23401 atagtatttt gcgccaaatt gccatgcaac gggcaatttg acgggcgtaa aagtttgaag
    23461 cagtggcaag acctgacaca gaggattccg attgatgtcg aatgctttgc gtagcgacac
    23521 tgatgccact actatcgttc cactttccat gacaataacg acagcctgat gccaaaatta
    23581 cgcctgatcg gattaacttt actcgcactt agcgcgactg ccgtctcaca cgctgaagaa
    23641 acgcgctatg tttctgacga actgaatacc tgggtccgta gcggtccggg agatcattat
    23701 cgcctcgtgg gcacggttaa cgccggcgag gaagtgacct tattacaaac tgacgccaac
    23761 accaattatg cccaggtgaa agacagctct ggccgtaccg cctggatccc gttgaaacaa
    23821 cttagcactg agccaagcct gcgctcccgt gtgccagatc tggaaaatca ggtcaaaacc
    23881 ctgaccgata aactcaccaa tatcgataac acctggaatc agcgcaccgc agaaatgcag
    23941 caaaaagtgg cgcagagcga cagcgtgatc aacgggttaa aagaagaaaa tcagaaactg
    24001 aaaaacgagc tgattgtcgc gcagaaaaag gtcgatgccg ccagcgtgca gctggatgac
    24061 aaacagcgca ccatcatcat gcaatggttt atgtatggcg gcggcgtgct ggggcttggc
    24121 ttgctgctcg gtctggtact gccgcacctg atcccaagcc gcaaacgcaa agatcgctgg
    24181 atgaactaaa tcgccttctt cgttgcactg tcttattatt aggaattgtg tatttgacga
    24241 agggatgatg gcgtgaagat ttatctggtc ggtggtgctg ttcgggatgc attgttaggg
    24301 ctaccggtca aagacagaga ttgggtagtg gtcggcagta cgccacagga gatgctcgac
    24361 gcgggctacc agcaggtagg ccgcgatttt cctgtgtttc tgcatccgca aacgcatgaa
    24421 gagtatgcgc tggcacgtac cgaacggaaa tccggctccg gttacaccgg ttttacctgc
    24481 tatgccgcac cggatgtcac gctggaagat gatcttaagc gtcgcgatct gaccattaat
    24541 gcgctggccc aggacgataa cggcgagatt atcgacccgt acaacggtct gggcgatctg
    24601 caaaatcgtc tgttgcgcca tgtttccccc gcttttggcg aagatccgtt acgcgtactg
    24661 cgcgtggcgc gttttgctgc gcgttatgcc cacctcggtt ttcgtattgc cgatgaaact
    24721 ctgacgctga tgcgcgagat gacccatgcg ggtgaactgg aacacctgac gcctgaacgg
    24781 gtatggaaag agacagaaaa cgccctcacc acccgcaatc cacaggtgtt cttccaggta
    24841 ctgcgcgatt gcggcgcact gcgcgtttta ttcccggaaa ttgacgccct gtttggtgtt
    24901 ccggcccctg ccaggtggca tccggaaatc gacacgggta ttcatacctt aatgacgctc
    24961 tcaatggcgg cgatgctgag tccgcaggtc gatgtccgtt tcacaacttt atgccacgat
    25021 ctcggtaaag gactgacgcc gccagaactc tggccgcgtc atcatggtca tggcccggcg
    25081 ggtgttaagt tagtggaaca actatgccag cgtctgcgcg tgccaaatga aattcgcgat
    25141 ttagccagac tggtggctga gtttcacgat ctcatccaca ccttcccaat gctgaaccca
    25201 aaaaccatcg tcaaattatt tgattccatc gatgcctggc gtaaaccgca gcgtgtcgag
    25261 caactggcgc tgaccagcga ggctgacgtg cgcggcagaa ccggttttga atcagcggac
    25321 tacccgcaag gccgctggtt gcgcgaagcc tgggaagtgg cgcagtcagt gccgacaaaa
    25381 gccgtcgttg aagcgggatt taaaggtgtg gagattcgcg aggagctgac ccgacggcgg
    25441 attgcggcgg tagccagctg gaaggaacaa cgttgcccaa agcctgagtg agtattgggc
    25501 attttgccgg atgcggcgta aacgccttat ccggctacat gtcaacgaca gttgtaggcc
    25561 tgataagacg cgcaagcgtc gcatcaagcg ttgattgccg gacgcggcgt gaacgcctta
    25621 ttcggcctac gcaatcgtgg acaacgccag gcatccgaca ctattcctca attaaaagaa
    25681 cacgacatac accgcagccg ccacaataaa gcgataaatg gcgaacggga taaacgaaat
    25741 gcgcttaatc aattgcagga aggttttaat cgctatcagc gccaccacaa aagcggtgat
    25801 aaacccaacg gcaaacatcg ggatatcgcc gcttgtcagg aagccccagc ttttgtagag
    25861 atcgagcgcc gttgcgccca tcatcatcgg caccgccagc aggaacgaaa actcggaagc
    25921 agcgtaacgg ctcaccccca tcagcatccc acctgaaatg gtcgccccgg aacgggagaa
    25981 acccggccac agcgccagac actggaaaca accaatcata aacgcctgac gataggtcat
    26041 atcatcaaga cccggcgcac gcggctcttt cggcttcagg cattcggcgg caatcagcaa
    26101 caaaccgcca acgaccagcg catacatcac atttatcggg ttaaacaatg acttaatcgt
    26161 gtcgtggaac aacagcccca gtaccaccgc cggaatcatc cccagcaaaa tgtggatcag
    26221 cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg cggccaaagt ggatgccaat
    26281 caggccaaac agacgccgcc agaacatcac tactaccgcc agaattgatc ctaactggat
    26341 cacaacttca aaggttttcg ccgtgtcgcc ctcaaacccc aacaagtgac cgacaataat
    26401 catatggccc gtgctggata ccggcagaaa ttctgtcaat ccttcgacca cacccaatat
    26461 tgccgctatc agcagcgagt gcatatcgct catcaataaa cccctaaatt ataaaaatgt
    26521 aacgcttgtc cgaactactg cagtatgacc aggttataac cgtttggttt aacagctgta
    26581 aaattaatta ttttctttca gattattgcc acgctcaatg attacgccaa cattcgccgc
    26641 ccgcgccact gcgcctggct tgctgagttt gatacgcacc cacggcgagt tgaagcgtgc
    26701 taacagcagc tctgccacct cttccgccac gcgctccacc agcgcaaaac gtgccccctc
    26761 gacgtggctg accaccgttt ctgcaatgtc agcgtagctg aggcaatccg ccacattatc
    26821 gctttttgcc gctttacggt tatcccacgc catttcgata tcgaacacta atttctgttc
    26881 gatggtctgt tcccagtcgt aaacaccaat agtggtgatt accgaaagtt gctctataaa
    26941 tacaatatcc atcacgacct gctcgctttt tggctaaccc ggataccact tccggcgaaa
    27001 tgtgcgtatt atccacagat tcatcgttta acacgaattt tcaaaacgga acagcttatg
    27061 agtgcaatcg cgcctggaat gatcctcatc gcgtacctct gcggctccat ttccagtgcc
    27121 attctggttt gccgcctgtg tgggctgccc gatccgcgaa ccagcggctc cggtaatcct
    27181 ggcgcaacta acgtgttacg tataggtggc aaaggagcag ccgtagcagt actgattttc
    27241 gacgttctga aaggaatgtt gcccgtctgg ggcgcgtatg aattaggtgt cagccccttc
    27301 tggctaggct taattgccat cgccgcctgt cttggacaca tctggcccgt tttcttcgga
    27361 tttaaaggag gaaaaggcgt tgctaccgct tttggtgcca tcgcacccat tggctgggat
    27421 ctcaccggag taatggcggg aacctggtta ctgaccgtgc tattgagcgg atactcgtcg
    27481 ctgggagcga ttgtcagtgc actgattgct ccgttttatg tctggtggtt taagccacaa
    27541 ttcaccttcc cggtttcgat gctctcttgc ctgatcctgc tgcgtcatca tgacaacatc
    27601 cagcgtctgt ggcgtcgtca ggagacaaaa atctggacga aattcaaaag aaagcgcgaa
    27661 aaggatcccg agtgatttct ggtggatcta catgacctga tagccttcat cgggtttgcc
    27721 cagccgttgc tggcaccatg ccgccagaaa ttccacgcag acacgtaatt tcatgctgcg
    27781 atagagcggc tcccggtaaa cagcccagat attggcgctt tgagcatact ccggcaatac
    27841 ttgcaccagt ttgccactct ccagaaacgg caacacatcc cactcggaac gcagcataat
    27901 ccctttgccc tccagcgccc attgcagcac aatctcgccg ctattggagg aaagatgccc
    27961 gctaaccttc accgattttt tctcctgacc gttccccaac tcccatattc cgtgggtcat
    28021 atcgcgctct ttggtcacca gacagtcatg acgacttaat tcctgtaggg attgtggctg
    28081 cggatatttt tgcagatact caggtgctgc gcataatatt cttttatttt tcgttaacag
    28141 atgcgcaata taataatcag gaatttcgtc attaatacga atatccagat caatattatc
    28201 ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatac acctgtaact caggataatt
    28261 gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa aactgcaacc
    28321 aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga ccagtcgctg
    28381 atattgggtg aggatttcca gcgcatgttc gtaacaacgt tggccacttt cagtcagcgc
    28441 cacgccgcga gcggagcggt tcagcagcgt tgttgccagc gtattttcaa ggatttggat
    28501 ccgcttggtg acaaatgcag gcgtttgccc aagcgtcgcc gctgcagcac taaaactccc
    28561 gctgtggacg atttctacca gtacctgcaa gtctttggct aaaggccagc tattcagcat
    28621 cgttaattat ccgcagttgt gataagcgca gtgtatttcg caaaacattg ccattccctg
    28681 ctctggcgca atattcattc ttattttcgc gttgattcac gaactgttaa ttacatattc
    28741 acggtggcaa aaaatataaa accacatttt tagtggtagt ttgtggcggt gaatttttcc
    28801 agacaaatac aaaaactgga gttgccatga tgagcgaaag taataagcaa caggcagtga
    28861 ataagttgac ggagattgtc gctaacttta ccgccatgat ttctacccga atgcctgatg
    28921 acgtggtgga taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg gggaaaatta
    28981 tctaccatac gatgttcgac aacatgcaaa aagcgattga cctgaatcgt cctgcctgtc
    29041 aggacaccgg ggagattatg ttcttcgtta aagtcggttc ccgcttccca ctgcttggcg
    29101 agctgcaaag catactcaaa caagccgtgg aagaggcgac catcaaagcg ccgctgcgtc
    29161 acaatgcggt agaaattttt gacgaagtaa acaccggcaa aaataccggt agcggcgtac
    29221 cgtgggtcac ctgggatatc gtccccgacg gtgacgatgc ggaaatcgaa gtttacatgg
    29281 caggcggcgg ctgcacgcta ccaggccgct cgaaagtgtt aatgccgtca gaaggctacg
    29341 aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct cgcagtaaac gcctgtccac
    29401 cggtactggt gggcgtgggc atcgccacct cggtggaaac cgccgccgta ctctcgcgta
    29461 aagccatttt gcgcccgatt ggcagccgcc accccaatcc aaaagcggca gagctggagc
    29521 tacgcctgga agaaggactc aaccgtctgg ggattggtcc acaagggctg actggcaaca
    29581 gttcagtgat gggcgtgcat atcgaatctg ccgcccgcca tccgtcaacc atcggcgttg
    29641 ctgtttctac cggttgctgg gcgcatcgtc gcggcacact gctggttcat gccgatctca
    29701 cctttgaaaa tctgtctcac acccggagcg cgttatgaaa aagatcctga caaccccgat
    29761 caaagctgaa gatctgcaag atattcacgt cggcgatgtg atctatctga ccggtacgct
    29821 ggtgacctgc cgtgacgttt gtcaccgccg attgatcgaa ctgaaacgtc cgctccctta
    29881 cgatctcaac ggcaaagcga ttttccacgc tggccccatc gtgcgcaaaa acggcgacaa
    29941 atgggagatg gtctccgtcg gtccgacaac cagtatgcgt atggaaagtt ttgaacgtga
    30001 atttatcgag cagaccggcg tgaaactggt ggtcggcaaa ggcggaatgg ggccgctgac
    30061 cgaagaaggc tgtcagaaat tcaaggcgct acatgtaatt ttcccggcag gctgcgcggt
    30121 gctggcggca acccaggtgg aagagattga agaagtgcac tggacagaac tcgggatgcc
    30181 ggagtcactg tgggtctgcc gggtcaaaga gttcggcccg ctgattgtct ctattgatac
    30241 ccacggcaac aacctgatag ccgaaaacaa aaagctgttc gccgaacgcc gcgatcccat
    30301 cgtggaagag atctgcgagc acgtccatta catcaaataa ccctcccgga gaggctcgcc
    30361 ccctctcctt tttcgcaggc ataacacgat gaaaccttcc actgaatggt ggcgatacct
    30421 tgcgccgctg gcggtcatcg ccattattgc tctaattccg gttcccgcag ggctggagag
    30481 tcatacctgg ctctactttg ccgtttttac tggcgtaatc gttggactga tcctcgaacc
    30541 cgtgccgggt gccgtggtgg cgatggtggg tatctccatc atcgccatac tctctccctg
    30601 gctgctgttc agcccggagc agctcgctca gccaggcttt aaattcactg caaaatccct
    30661 ctcgtgggcc gtttccggtt tttctaattc ggttatctgg ctgattttcg ccgcctttat
    30721 gtttggcaca ggctatgaaa aaaccgggct ggggcgacgt atcgcgctga ttctggtgaa
    30781 aaagatgggg catcgcacgc tgtttcttgg ctatgcggtg atgttctccg agctgatcct
    30841 ggcacctgtc acaccgtcca actcggcgcg tggtgcgggg attatctatc ccatcatccg
    30901 taacctgccg ccgctctatc aatcacaacc aaacgacagc agttcgcgca gtattggctc
    30961 gtacatcatg tggatgggga ttgttgccga ctgcgtgacc agcgccattt tcctgacggc
    31021 gatggctcct aacttgctgt taattggact gatgaaaagc gcatctcacg ccacgctgag
    31081 ttggggcgac tggttcctcg ggatgttgcc gctcagtatt ttactggtcc tgctggttcc
    31141 ctggctggct tacgtgctgt acccgcctgt actgaagtca ggcgatcagg tgccgcgctg
    31201 ggcagagacg gaactgcagg caatgggccc gctctgttcg cgtgaaaaac ggatgctggg
    31261 gctgatggta ggcgcgctgg tgctgtggat tttcggcggt gattatatcg atgccgcgat
    31321 ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc attatcagtt gggacgatat
    31381 tgtcagtaat aaagcggcgt ggaacgtttt cttctggctg gcctcgctta tcaccctcgc
    31441 gaccggactc aacaacaccg gttttattag ctggtttggc aaactgttag caggcagctt
    31501 aagcggttat tcgccaacga tggtgatggt ggcgttgatt gtggtgtttt atctactgcg
    31561 ctactttttt gccagcgcca cggcgtatac ctccgctctc gcaccgatga tgattgccgc
    31621 tgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc ctgatggttg gtgcggcaat
    31681 tggtctgggg agcattctta caccatacgc caccggcccc agtccgattt actacggcag
    31741 tggttatctg ccaacggcgg attactggcg actgggggcg atttttgggc tgatattcct
    31801 cgtattgctg gtgattaccg gcttactgtg gatgcccgtg gtgttgcttt aagtaaggac
    31861 ggtgccggaa gtaactctcc ggcacccgat agttttacgc agccggtaac tccgccagcg
    31921 gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc gcctgcttta aaccgcacca
    31981 ttccggcata ggcgatcatc gcgccgttat cagtacaaaa ttccggacgc gcgtagaaca
    32041 cttcgccgcg gcgttttttc atcatttcag ccagcttcgc ccgtaacgtg cggttagcac
    32101 tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg atccagcgca cgcttacact
    32161 taatcatcaa cgtatcgacc accgcatctt caaaggcgcg ggcgatgtca gcacgcgtct
    32221 ggtcgtcggt gccgttgtca cgaatggtat ttgccgcgaa ggttttcagg ccggagaagc
    32281 tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa aacaaagcgc ccggcagtac
    32341 cctgcgccgc cattttcgac agtaacggtc cgccaggata atccagcccc agcagcttcg
    32401 cggttttatc aaacgcttca ccggcggcat catcgataga ctcgccgagc agctcgtact
    32461 gaccaatgcc agtcacgctg attaactgcg tatgaccgcc ggaaaccagc agcgcaacaa
    32521 acggaaattc cggcgggtta tcttccagca tcggcgctaa cagatgccct tccatatggt
    32581 gtacagggat cgccggaacg ttccaggcaa acgccagaga acgccccacg gtcgcgccaa
    32641 ccagcagtgc gccgactaat ccagggcctg cggtataggc cacagcatca atgtcttttg
    32701 ccgttaaacc agactccttt agcgccgcct ggatcaacgg tacggtttta cgcacatgat
    32761 cgcgggaggc cagttcaggc acaacgcccc cgtagtcagc gtgcaatttc acctgactat
    32821 acaattggtt ggctaacaaa cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc
    32881 aggaagtttc aatacccagt acacgcatga cttgttttac ctcgctttat taccgcgcag
    32941 tgtaggacca atgcgggttg atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat
    33001 actcctcacc cttataaaag tccctttcaa aaaaggccgc ggtgctttac aaagcagcag
    33061 caattgcagt aaaattccgc accattttga aataagctgg cgttgatgcc agcggcaaac
    33121 cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa gtacgtgaaa acgagccgtt
    33181 cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa gcaggtgttc tggcggaagt
    33241 tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt aagcgcgcta aagcttctgc
    33301 agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca cgccgcactc gtctgtacta
    33361 attccccgag agcgttgctc tccgatcaga ccgagttgta gttgtaaggc cgtgcttccg
    33421 aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa aatcggggcc tatggctgga
    33481 cgaatcccac gcgtattcat taatgatctg ctggcacgca ctgacatcgt cgatctgatc
    33541 gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc acgcgtgttg tccattccac
    33601 aacgagaaaa ccccgtcctt caccgttaac ggtgagaaac agttttacca ctgctttgga
    33661 tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact acgacaagct tgagttcgtc
    33721 gaaacggtcg aagagctggc ggcaatgcac aatcttgaag tgccatttga agcaggtagc
    33781 ggccccagcc agatcgagcg ccatcagcgg caaacgcttt atcagttgat ggacggtctg
    33841 aatacgtttt accaacaatc tttacaacaa cctgttgcca cgtctgcgcg ccagtatctg
    33901 gaaaaacgcg gattaagcca cgaggttatc gctcgctttg cgattggttt tgcgcctccc
    33961 ggctgggaca acgtcctgaa gcggtttggc ggcaatccag aaaatcgcca gtcattgatt
    34021 gatgcgggga tgttggtcac taacgatcag ggacgcagtt acgatcgttt ccgcgagcgg
    34081 gtgatgttcc ccattcgcga taaacgcggt cgggtgattg gttttggcgg gcgtgtgctg
    34141 ggcaacgata cccccaaata cctgaactcg ccggaaacag acattttcca taaaggccgc
    34201 cagctttacg gtctttatga agcgcagcag gataacgctg aacccaatcg tctgcttgtg
    34261 gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg gcattaatta cgccgttgcg
    34321 tcgttaggta cgtcaaccac cgccgatcac atacaactgt tgttccgcgc gaccaacaat
    34381 gtcatttgct gttatgacgg cgaccgtgca ggccgtgatg ccgcatggcg agcgctggaa
    34441 acggcgctgc cttacatgac agacggccgt cagctacgct ttatgttttt gcctgatggc
    34501 gaagaccctg acacgctggt acgaaaagaa ggtaaagaag cgtttgaagc gcggatggag
    34561 caggcgatgc cgctttccgc atttctgttt aacagcctga tgccgcaagt tgatctgagt
    34621 acccctgacg ggcgcgcacg tttgagtacg ctggcactgc cattgatatc gcaagtgccg
    34681 ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca acaaattagg catacttgat
    34741 gacagccagc ttgaacgatt aatgccaaaa gcggcagaga gcggcgtttc tcgccctgtt
    34801 ccgcagctaa aacgcacgac catgcgtata cttatagggt tgctggtgca aaatccagaa
    34861 ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa ataagctccc tggacttggc
    34921 ttattcagag aactggtcaa cacttgtctc tcccagccag gtctgaccac cgggcaactt
    34981 ttagagcact atcgtggtac aaataatgct gccacccttg aaaaactgtc gatgtgggac
    35041 gatatagcag ataagaatat tgctgagcaa accttcaccg actcactcaa ccatatgttt
    35101 gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc gtgagcgcac gcatggttta
    35161 agcaacgaag aacgcctgga gctctggaca ttaaaccagg agctggcgaa aaagtgattt
    35221 aacggcttaa gtgccgaaga gcgatcggga agcccccgac agccgcactg agaggcagcg
    35281 gcaaatatat aagtacgccc tcgtaattat cgttggcggt aaacaaccgt tggatttcag
    35341 cgttaacggc tgaaggacat cgggtcaatc gcccaacacc aacctcatga aataagtgtg
    35401 gataccgtct tatggagcaa aacccgcagt cacagctgaa acttcttgtc acccgtggta
    35461 aggagcaagg ctatctgacc tatgccgagg tcaatgacca tctgccggaa gatatcgtcg
    35521 attcagatca gatcgaagac atcatccaaa tgatcaacga catgggcatt caggtgatgg
    35581 aagaagcacc ggatgccgat gatctgatgc tggctgaaaa caccgcggac gaagatgctg
    35641 ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga aatcgggcgc acgactgacc
    35701 cggtacgcat gtacatgcgt gaaatgggca ccgttgaact gttgacccgc gaaggcgaaa
    35761 ttgacatcgc taagcgtatt gaagacggga tcaaccaggt tcaatgctcc gttgctgaat
    35821 atccggaagc gatcacctat ctgctggaac agtacgatcg tgttgaagca gaagaagcgc
    35881 gtctgtccga tctgatcacc ggctttgttg acccgaacgc agaagaagat ctggcaccta
    35941 ccgccactca cgtcggttct gagctttccc aggaagatct ggacgatgac gaagatgaag
    36001 acgaagaaga tggcgatgac gacagcgccg atgatgacaa cagcatcgac ccggaactgg
    36061 ctcgcgaaaa atttgcggaa ctgcgcgctc agtacgttgt aacgcgtgac accatcaaag
    36121 cgaaaggtcg cagtcacgct gccgctcagg aagagatcct gaaactgtct gaagtattca
    36181 aacagttccg cctggtgccg aagcagtttg actacctggt caacagtatg cgcgtcatga
    36241 tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct ctgcgttgag cagtgcaaaa
    36301 tgccgaagaa aaacttcatt actctgttta ccggcaacga aaccagcgat acctggttca
    36361 acgcggcaat tgcgatgaac aagccgtggt cggaaaaact gcacgatgtc tctgaagaag
    36421 tgcatcgcgc cctgcagaaa ctgcagcaga ttgaagaaga aaccggcctg accatcgagc
    36481 aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc gaaagcccgc cgtgcgaaga
    36541 aagagatggt tgaagcgaac ttacgtctgg ttatttctat cgccaagaaa tacaccaacc
    36601 gtggcttgca gttccttgac ctgattcagg aaggcaacat cggtctgatg aaagcggttg
    36661 ataaattcga ataccgccgt ggttacaagt tctccaccta cgcaacctgg tggatccgtc
    36721 aggcgatcac ccgctctatc gcggatcagg cgcgcaccat ccgtattccg gtgcatatga
    36781 ttgagactat caacaaactc aaccgtattt ctcgccagat gctgcaagag atgggccgtg
    36841 aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc ggaagacaag atccgcaaag
    36901 tgctgaagat cgccaaagag ccaatctcca tggaaacgcc gatcggtgat gatgaagatt
    36961 cgcatctggg ggatttcatc gaggatacca ccctcgagct gccgctggat tctgcgacca
    37021 ccgaaagcct gcgtgcggca acacacgacg tgctggctgg cctgaccgcg cgtgaagcga
    37081 aagttctgcg tatgcgtttc ggtatcgata tgaacaccga tcacacgctg gaagaagtgg
    37141 gtaaacagtt cgacgttacc cgtgaacgta tccgtcagat cgaagcgaag gcgctgcgca
    37201 aactgcgtca cccgagccgt tctgaagtgc tgcgtagctt cctggacgat taatcggtag
    37261 gccggatcag gcgttacgcc gcacccggca cctgtccctt tgcacaaacg ccaccttttc
    37321 ggtggcgttt tttatcgccc acgcaccacc agcgcctggt ccagctcgcg atacgcttca
    37381 accagtttct ccagtgaaac gcgacttaaa ccgctgggat ttggcagcac ccaaatctgc
    37441 gtcgaaccaa tgctgagcgt ttgtttcccc cactgtgcac cacgctggct gaatccctgt
    37501 tcatatgctt gtttgcccag aatcgccaac gcttgcggct gataatcttc aattttttca
    37561 atcagcttac gcccacctgc gtgtagctct tgctttgaaa tttcattggc ttgcaccgtt
    37621 ggcctgtcta ccagtttggt gacgccacaa cgataatcca gcagatgctg tgcctcctgc
    37681 ggcttcaact gatggtcggt aaacccggcc tgatatatca ccttccagaa gcgatttgcc
    37741 ggatgagcaa agggaaaacc agtcccggcg gatgaaagcc cagggttgat accgcaaaac
    37801 acgacccgta accctggagc caaaatatcc tcaaccatat ttactccttt ctaacatcac
    37861 caggaaaagt ataacggatt gaaaatacat tgtttataaa aacagcaggc gcgcggtaat
    37921 ggctggattg cgacacggag ttactttata atccgctacc atggcccctt agctcagtgg
    37981 ttagagcagg cgactcataa tcgcttggtc gctggttcaa gtccagcagg ggccaccaga
    38041 tatagcaaag gctgacgaga aatcgtcagc ctttttcttt ttatatatca gttactttgc
    38101 gtgccagtag gccgctgcac gtacccgctg tgggtcatac tgttccgctt caaagcggcg
    38161 gcttaaattc ttaacgactt taccttcgcc ggttatccag atgaagtaat catcggcagg
    38221 gatttgcatc tgcgccagac gcgcatctac cgcctgctca tcatgtgcca gccattcgat
    38281 attaaaacca tcaaggtgcg cgagataatc ctgacaggcg ttatcccgca cgctaaccag
    38341 cgcactaact tgcggtttaa cggcaagttt gctcaacgtt tccaggcggc ggcgcaatgc
    38401 gggcattccg gattcatcgc agacatacag ctgatacgcg taatcttccg gcaccaccag
    38461 cgaaccgcgc ggacctgcca ccgtaagttt atcgcccggt tgcgcctgca tcgcccagcc
    38521 gctggcgacc ccaccgtcgt gaataaagaa atcgatcgcc agttcatggc gtagttcgtc
    38581 atacagcggc gtatagtcac gcgacggtgg gcgtggtcct tccggccaga cgatgccctc
    38641 ttccgttacc gttggcggca caaagtgagc gtcaggttga ggaaagaaga gtttgctgtg
    38701 atcgtcaaag ccgcgcgatg taaaaccgtc cagcgcctcg ccgccaagga caatgcgctg
    38761 aaaaccggcg ctgatacgct caacgcgtag cacagtcaat tcacggaagc gcagatcatt
    38821 gcgaacgcgc tgcgggtagc ggggggagtt attcattgtt atcgccttcg tgatggtaat
    38881 cagatatatc taaataattt tcgcaaatga taatgattgt taatcatgat aaatgcaagc
    38941 gatttgtaga actgatatgt ctatagtctg acaagacgaa ccgcctcttc tcaggcatca
    39001 ttactcaacg ccggatgcgg cgtgaacgcc ttatccggcc tacgtgtgag atgagtccac
    39061 atttaaaaat catttttatt cttgcaagag tgctcacatc agatataaat tagatatatc
    39121 taattaagca aaaggaggct gatatgagcc atcatcacga agggtgttgt aaacatgaag
    39181 gccagccacg ccatgagggc tgctgcaaag gtgagaagtc agaacacgag cactgcggac
    39241 acggtcacca gcatgaacac ggtcaatgtt gcggtggtcg ccacggtcgc ggcggcggtc
    39301 gtcggcaacg tttctttggt cacggtgaat tgcgtctggt gattctggat attctctcgc
    39361 gcgatgacag ccacggttac gaattgatta aagcgattga gaatctaacc caagggaatt
    39421 acaccccaag cccgggcgtc atctacccga cgctggattt tctgcaggag cagtcgctga
    39481 ttaccatccg cgaagaggaa ggtggtaaga agcagattgc gctgaccgaa caaggcgcgc
    39541 agtggctgga agaaaaccgc gaacaggtgg agatgattga agaacgcatc aaagcgcgtt
    39601 gcgttggcgc tgcgctgcgc cagaacccgc aaatgaagcg ggcgctagat aattttaaag
    39661 cggtgctgaa tttacgcgtc aaccagagtg atatcactga tgcacaaata aaaaagatca
    39721 ttgcggtgat cgaccgcgcc gcttttgata ttacgcaact ggattaatcg ccgcatccgc
    39781 cagtggcgcg gtgcaattgc cggatgcgac gcttgacgca ccttatccgg cctacacccg
    39841 ctacacaccc cgtaggcctg ataagatgcg ccagcatcgc atcaggcatc gtgtatcaaa
    39901 taccggatcc ggcataccga ttaatgcagc accgtcaccg cgtcttccag tcggctggcg
    39961 cggtgtttca ccatcgccga cacctgcgca ctctcttcca ccagctcggc atttttctgg
    40021 gtgatgaggt taagctcatc cactgcacgg gtcaggctgg aaagaccatc ggcctgttcc
    40081 agcgttgaat ggctaatctg ggcaatcaac tgggtgacgt ttttcacctg tgccacaata
    40141 tcttccatcg tacgtccggc ggcgtgtacc tgctgcgaac cggattgcac cttatcagca
    40201 ctggcatcaa tcagcttgcg aatatcgttg gcagcattgg cgctgcggct ggctaaatga
    40261 cgcacttccc ccgccaccac cgcaaaacct ttgccctgtt cgccggcacg cgccgcttcc
    40321 accgccgcat tcagggccag aatattggtc tgaaacgcaa tatcgttaat cagcgaagta
    40381 atggtgccaa tgcgctgggt actgtcggcg atatcgtcca ttgtcttgat caccgtggtc
    40441 atcgcctccc cgccctgcac tgccgcatta ctggcggtga ttgacagttt atcggcagcc
    40501 gacgccgtgg cagagttctg tttcaccgac gccgccattt ggttcatggt cgccaccgtt
    40561 tgctgaacgt tatcaactgt ctgctgggta tgttcgttca gttcatcggt gcctttcgcc
    40621 agcgtctcac tgccgtttct gacactggac acctggcttg agacatcgtt aattaaccaa
    40681 cggcacatca ggccaagctg ccctaccgcg cgtaatgtca gccccagctc atcgctgcga
    40741 ttcagatgct caacactatt acgctctccg gtcgccacct tcagtgcctg acgggcgaca
    40801 ttttctatcg ggcggacaat ctgccattca aaacaggcgc ttgccaacaa taccactaac
    40861 gcacagagga tatacgtcac caccggggca gcaacaaacc aaagcatggc cgccagcaag
    40921 ataaacatca gggtcatcac tccacgcgcc cgccagcgaa gcggtaatga aggcagttta
    40981 cccagccagc ctttacgcac caccaggcct ttatgaatac gcttaccggt acgtccggcg
    41041 ttcagcgctt tgtacagcgg ctccaccgcc gcgatctctt catccgtcgc ccgggtacga
    41101 atcgacatat agccactgat ttttccctcg cgcaccatcg gtaccgcatt ggcccgcacc
    41161 caataatggt caccattttt gcggcgattt ttcacgatgc cgctccaggg ctcccctttt
    41221 ttcagggtga accacatatc cgcaaacgcc gcttttggca tatccgggtg acgcaccatg
    41281 ttgtgcggct gcccttgtaa ctcttgcaag gtaaagccgc tcacctgcac aaaagtgtca
    41341 ttagcatgag tgatatagct ttgcagatcg gtagtggaca tcagagtggt atcgtccgcc
    41401 agcggggtat tttgctgggt gacatacgga tgagaagaca tgttcgcgtc ctgtgcaggt
    41461 tatatggttg ttaacttctt gtcagagttt atgtcggccc cgctgcggtt atctttaacc
    41521 gattaatttg atttagatcg caatttgcga ttaaagcaca aatctaattc gttgatttaa
    41581 aatactttca ctctgttact atacgaaaac gttaattatc ttgcccaaaa atcaggcagt
    41641 taatgccctg aaagcgtgca tttgcgcagc aattatcaaa tccatacccg acaaaaaccg
    41701 tgcaaaataa caacagatgt taacaaatag cattaaatat tgcgcaaatg ataaccacat
    41761 ttgtgtttat cccgattttc gcgatcgcag ccggagtggc gcaatccctg caatacttaa
    41821 atcggtatca tgtgatacgc gagcctccgg agcatatttt gaacaggtta ccttcgagcg
    41881 catcggcttt agcgtgcagc gcccacgccc tgaatctcat tgaaaagcga acgctggatc
    41941 atgaggagat gaaagcactt aaccgagagg tgattgaata cttcaaagag catgtcaatc
    42001 cggggttttt agagtatcgc aaatctgtta ccgccggcgg ggattacgga gccgtagagt
    42061 ggcaagcggg aggtttaaat acgcttgtcg acacccaggg acaggagttt atcgactgcc
    42121 tgggaggttt tggaattttc aacgtggggc accgtaatcc agttgtggtt tccgccgtac
    42181 agaatcaact tgcgaaacaa ccactgcaca gccaggagct actcgatccg ttacgggcta
    42241 tgctggcgaa aacccttgct gcgctaacgc caggtaaact gaaatacagc ttcttctgta
    42301 atagcggcac cgagtccgtt gaagcggcgc tgaagctggc gaaggcttat cagtcaccgc
    42361 gcggcaaatt tacttttatt gccaccagcg gcgcgttcca cggtaaatca cttggcgcgc
    42421 tgtcggccac ggcgaaatcg accttccgca aaccgtttat gccactgctg ccagggttcc
    42481 gtcatgtgcc gtttggcaat atcgaagcca tgcgcacggc tcttaatgag tgcaaaaaaa
    42541 ccggtgatga tgtggccgcg gtgatcctcg aaccgattca gggcgaaggt ggcgtaattc
    42601 tgccgccgcc gggctatctc accgccgtgc gtaagctgtg cgatgagttc ggcgcactga
    42661 tgatcctcga tgaagtacaa acgggcatgg ggcgcacggg caagatgttc gcctgcgaac
    42721 atgagaacgt acagccggac atcctgtgcc tggcaaaagc cctcggcggc ggtgtgatgc
    42781 cgattggcgc aaccatcgcc actgaagagg tgttctcagt tctgttcgac aacccgttcc
    42841 tgcataccac cacctttggc ggcaacccgc tggcctgtgc ggcggcgctg gcgaccatca
    42901 atgtgttgct ggagcagaac ttaccggctc aggctgagca aaaaggcgat atgttgctgg
    42961 acggtttccg tcaactggcg cgggaatatc ccgatctggt acaggaagcg cgtggtaaag
    43021 ggatgttaat ggcgattgag tttgttgata acgaaatcgg ctataacttt gccagcgaga
    43081 tgttccgcca gcgcgtactg gtggccggaa cgctcaataa cgccaaaacg atccgcattg
    43141 aaccgccact gacactgacc attgaacagt gtgaactcgt gatcaaagcg gcgcgtaagg
    43201 cgctggcggc catgcgagta agtgtcgaag aagcgtagta tcaaatcgga tggcaatacg
    43261 acgtcgccat ccgatctttt tatacgatac gtacgcccgc aggcatcatc cgctccggcg
    43321 ttaacagcac gctttcactg ccgtcatcgg tttccgcaca caacagcatg cattccgacg
    43381 tttcaccacg cattttcgct ttttgcagat tgcataagac caccaccgtt tttcccatca
    43441 actcttcttc gctgtagtac ggcaccaggc tggtcacggt ttgcagtgtt ttttccccca
    43501 catcaacctg tacgatgtac agcttgtcgg cgttttcatg gcgtttcact tccacaatct
    43561 ttccgacgcg catttccaga cgtgcaaaat cagcgtaagc cacggtttcc attgcttcct
    43621 cccttcgagt aaaattttac taaactatag caaaagtttt tctcaatcct gtaggctaaa
    43681 aatggagaat gcaggcgtga tcacattcgt gagccgctgt gttaccgtta cagcgtcaaa
    43741 gaaacgcgct ttatttactg aaaacaggtg acccgataag cacttcctct acaatggggg
    43801 cgcacatcag ggaaagtaaa aaaggtaaac atggcaacac taaaagacat cgcaatcgaa
    43861 gctggcgtat ccctggcgac agtatccagg gtcttaaatg acgatccgac attgaatgtg
    43921 aaagaagaga cgaaacatcg cattctcgag atcgccgaaa agctggagta caagaccagt
    43981 agcgcccgta aactacagac aggcgcagtc aaccaacacc atattctggc tatctacagc
    44041 taccagcagg agctggagat caacgatcct tactatctgg cgatccgcca cggcattgaa
    44101 actcagtgcg aaaagctggc gatcgaactg accaactgtt atgaacacag cggattacca
    44161 gacattaaaa acgtcaccgg tattttaatt gtcggcaaac ccacgcccgc cctgcgcgcc
    44221 gctgccagcg cgttgaccga caatatctgt tttatcgact ttcacgaacc cggcagcggt
    44281 tacgatgcgg tggatatcga tctggcacgc atcagtaaag aaatcatcga cttctatatc
    44341 aaccagggcg ttaatcgtat tggttttatt ggcggtgaag atgagcctgg caaggcggat
    44401 attcgtgagg tcgcctttgc ggaatatggc cgactgaaac aagtggtacg cgaagaggat
    44461 atctggcgcg gcggtttttc cagttcgtcg ggttatgaac tggcaaaaca aatgctggcg
    44521 cgggaagact atccgaaggc actgtttgtt gcttccgatt ccattgctat cggcgtactg
    44581 cgggcgattc atgaacgtgg tctgaacatc ccacaggata tttcgcttat cagcgttaac
    44641 gatatcccca ccgcgcgatt tacctttccg ccgctctcca ccgtgcgcat ccattccgaa
    44701 atgatgggaa gtcagggcgt aaacctggtg tatgaaaaag cccgcgatgg tcgcgcgctg
    44761 ccgctgttag tcttcgttcc cagcaaatta aaactgcgcg gcacgacccg ttaaatcccc
    44821 ttacacactg tccggcaatc gtttttgccg ggcagtgctg ccgtttattt tcgtgatcca
    44881 gttaaagtaa atgcatttac ccgctacttt ttagtaaaaa ttttactaaa ctccccagca
    44941 attacacaaa ctaccatcac catgaatggt tccgatttct ctctaccggg aggccctatg
    45001 aatcgctggg aaaacattca gctcacccac gaaaaccgac ttgcgccgcg tgcgtacttt
    45061 ttttcatatg attctgttgc gcaagcgcgt acctttgccc gcgaaacgag cagcctgttt
    45121 ctgcccttaa gcggtcagtg gaatttccac ttttttgacc atccgctgca agtgccagaa
    45181 gccttcacct ctgagttaat ggctgactgg gggcatatta ccgtccccgc catgtggcaa
    45241 atggaaggtc acggcaaact gcaatatacc gacgaaggtt ttccgttccc catcgatgtg
    45301 ccgtttgttc ccagcgataa cccaaccggt gcctatcaac gtattttcac cctcagtgac
    45361 ggctggcagg gtaaacagac gctgattaaa tttgacggcg tcgaaaccta ttttgaagtc
    45421 tacgttaacg gtcagtatgt gggtttcagc aagggcagtc gcctgaccgc agagtttgac
    45481 atcagcgcga tggttaaaac cggcgacaac ctgttgtgtg tgcgcgtgat gcagtgggcg
    45541 gactctacct acgtggaaga ccaggatatg tggtggtcag cggggatctt ccgcgatgtt
    45601 tatctggtcg gaaaacacct aacgcatatt aacgatttca ctgtgcgtac cgactttgac
    45661 gaagcctatt gcgatgccac gctttcctgc gaagtggtgc tggaaaatct cgccgcctcc
    45721 cctgtcgtca cgacgctgga atataccctg tttgatggcg aacgcgtggt gcacagcagc
    45781 gccattgatc atttggcaat tgaaaaactg accagcgcca gctttgcttt tactgtcgaa
    45841 cagccgcagc aatggtcagc agaatcccct tatctttacc atctggtcat gacgctgaaa
    45901 gacgccaacg gcaacgttct ggaagtggtg ccacaacgcg ttggcttccg tgatatcaaa
    45961 gtgcgcgacg gtctgttctg gatcaataac cgttatgtga tgctgcacgg cgtcaaccgt
    46021 cacgacaacg atcatcgcaa aggccgcgcc gttggaatgg atcgcgtcga gaaagatctc
    46081 cagttgatga agcagcacaa catcaactcc gtgcgtaccg ctcactaccc gaacgatccg
    46141 cgtttttacg aactgtgtga tatctacggc ctgtttgtga tggcggaaac cgacgtcgaa
    46201 tcgcacggct ttgctaatgt cggcgatatc agccgtatta ccgacgatcc gcagtgggaa
    46261 aaagtctacg tcgagcgcat tgttcgccat attcacgcgc agaaaaacca tccgtcgatc
    46321 atcatctggt cgctgggcaa tgaatccggc tatggctgta acatccgcgc gatgtaccac
    46381 gcagcgaagg cgctggatga cacgcgactg gtgcattacg aagaagatcg cgatgctgaa
    46441 gtggtcgata ttatttccac catgtacacc cgcgtgccgc tgatgaatga gtttggtgaa
    46501 tacccgcatc cgaagccgcg catcatctgt gaatatgctc atgcgatggg gaacggaccg
    46561 ggcgggctga cggagtacca gaacgtcttc tataagcacg attgtattca gggacattat
    46621 gtttgggagt ggtgcgacca cgggatccag gcgcaggatg acaacggcaa tgtctggtat
    46681 aaattcggcg gcgactacgg cgactatccc aacaactata acttctgtct tgatggtttg
    46741 atctattccg atcagacgcc gggaccaggc ctgaaagagt acaaacaggt tatcgcgccg
    46801 gtaaaaatcc acgcgctgga tctgactcgc ggcgagctga aagtcgaaaa taaactgtgg
    46861 tttaccacgc tggatgacta caccctgcac gcagaggtgc gcgccgaagg tgaaacgctc
    46921 gcgacgcaac agattaaact gcgcgacgtt gcgccgaaca gcgaagcccc cttgcagatc
    46981 acgctgccgc agctggacgc ccgcgaagcg ttcctcaaca ttacggtgac caaagattcc
    47041 cgcacccgct acagcgaagc cgggcattct atcgccactt atcagttccc gctgaaggaa
    47101 aacaccgcgc agcctgtacc tttcgcaccg aataacgccc gtccgctgac gctggaagac
    47161 gatcgtttga gctgtactgt tcgcggctac aactttgcga tcaccttctc aaaaacgagt
    47221 ggtaaaccga catcctggca ggtaaatggc gaatcgctgc tgacccgcga gccaaagatc
    47281 aacttcttca agccaatgat cgacaaccac aagcaggagt acgaagggtt gtggcagccg
    47341 aatcatttgc agatcatgca ggaacatctg cgcgactttg ccgtagaaca gagcgatggt
    47401 gaagtgttga tcatcagccg cacggttata gcaccgccgg tgtttgactt cgggatgcgc
    47461 tgtacctaca tctggcgcat cgctgcagat ggccaggtta acgtggcgct ttccggcgag
    47521 cgttacggcg actatccgca catcattccg tgcatcggtt tcaccatggg gattaacggc
    47581 gaatacgatc aggtggcgta ttacggtcgt ggaccgggcg aaaactacgc cgacagccag
    47641 caggctaaca tcatcgatat ctggcgcagc accgtcgatg ccatattcga gaactatccc
    47701 ttcccgcaga acaacggcaa ccgtcagcat gtccgctgga cggcactgac taaccgccac
    47761 ggcaacggtc tgctggtggt tccgcagcgc ccaattaact tcagcgcctg gcactatacc
    47821 caggaaaaca tccacgctgc ccagcactgt aacgagctgc agcgcagtga tgacatcacc
    47881 ctgaatctcg accaccagct gcttggcctc ggctccaatt cctggggcag cgaggtgctg
    47941 gactcctggc gcgtctggtt ccgtgacttc agctacggct ttacgttgct gccggtttct
    48001 ggcggagaag ctaccgcgca aagcctggcg tcgtatgagt tcggcgcagg gttcttttcc
    48061 acaaatttgc acagcgagaa taagcaatga ggatcatcga taacttagaa cagttccgcc
    48121 agatttacgc ctctggtaag aagtggcaac gctgcgttga agcgattgaa aatatcgaca
    48181 acattcagcc tggcgtcgcc cactccatcg gtgactcatt gacttaccgc gtggagacag
    48241 actccgcgac cgatgcgcta tttaccgggc atcgacgcta ttttgaagtg cattactacc
    48301 tgcaagggca gcaaaaaatt gaatatgcgc cgaaagagac attacaggtc gtggaatatt
    48361 atcgtgatga aactgaccgt gaatatttaa aaggctgcgg agaaaccgtt gaggtcgacg
    48421 aagggcaaat cgttatttgc gatatccatg aagcatatcg gtttatctgc aataacgcgg
    48481 tcaaaaaagt agttctcaaa gtcaccatcg aagatggtta tttccataac aaataacaac
    48541 tacggcggca aaaggagttt gccgccaccg ctaccctact cattttcgga gatgtgttat
    48601 gtctgatacc aaacgtaata caatcggcaa attcggcttg ctctcgctga cttttgccgc
    48661 cgtttacagc tttaacaacg ttatcaataa taatattgag cttggactgg cctcggcacc
    48721 gatgtttttc ctcgcgacga ttttttattt tattcccttc tgtctgatca tcgcagaatt
    48781 tgtttcgtta aataaaaact cagaagccgg tgtctacgcg tgggtaaaaa gttcgctggg
    48841 cggacgttgg gcatttatta ctgcctatac ctactggttc gtaaacctgt tctttttcac
    48901 ctcgctgttg ccgcgcgtta ttgcttatgc ttcgtatgcc ttcctcggct atgaatatat
    48961 tatgacgccg gttgccacca ccattatcag tatggtgctg ttcgccttct ccacctgggt
    49021 ttccaccaac ggggcgaaaa tgttggggcc aattacctcc gtcacttcaa cgctgatgct
    49081 gctgttaacg ctctcctaca ttttactggc aggtacggcg ctggttggcg gcgtacagcc
    49141 tgctgacccc atcaccgttg acgcgatgat cccgaacttc aactgggcgt tcctcggcgt
    49201 taccacctgg atctttatgg ccgcaggtgg cgcggagtcc gtcgctgtgt acgttaacga
    49261 cgtcaaaggc ggttcgaaat cgttcgttaa agtgatcatc ctcgccggga tttttatcgg
    49321 tgtactatat tccgtctcct cggtgctgat taacgtcttc gtcagcagca aagagttgaa
    49381 atttactggc ggatcggtgc aggtattcca cggcatggcg gcgtattttg gtctgccgga
    49441 agcgttgatg aatcgctttg tcggtctggt gtcctttacc gcaatgttcg gttccctgct
    49501 gatgtggacc gcaacgccgg tgaaaatttt cttctccgaa atcccggaag gcatctttgg
    49561 taagaaaacc gtcgaactta acgaaaacgg cgttccggcg cgcgcagcgt ggatccagtt
    49621 cctgatcgtc atcccgctga tgattatccc gatgctcggt tccaatactg tgcaggatct
    49681 gatgaatact attattaata tgaccgccgc agcgtccatg cttccgccgt tattcatcat
    49741 gctggcttac ctgaatttac gcgccaaatt agatcacctg ccacgcgatt tccgtatggg
    49801 ctcccgccgc accggtatta tcgttgtttc aatgctgatt gcgatatttg ccgtagggtt
    49861 tgtcgcttcg acattcccga ctggcgcgaa tattctgacc atcatttttt ataacgtcgg
    49921 cggtattgtt atattccttg gctttgcgtg gtggaaatac agtaaatata taaagggatt
    49981 aacggctgaa gagcgccata ttgaagcgac gccagccagc aatgtt
//
LOCUS       DAZFBR010000021        48911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFBR010000021 DAZFBR010000000
VERSION     DAZFBR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.021"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            431..1648
                     /gene="alaA"
                     /locus_tag="ACHJZ2_001397"
     CDS             431..1648
                     /gene="alaA"
                     /locus_tag="ACHJZ2_001397"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO4567543.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1732..2331
                     /gene="yfbR"
                     /locus_tag="ACHJZ2_001398"
     CDS             1732..2331
                     /gene="yfbR"
                     /locus_tag="ACHJZ2_001398"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4567544.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2390..4222)
                     /locus_tag="ACHJZ2_001399"
     CDS             complement(2390..4222)
                     /locus_tag="ACHJZ2_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO4567545.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4309..4959)
                     /gene="hxpA"
                     /locus_tag="ACHJZ2_001400"
     CDS             complement(4309..4959)
                     /gene="hxpA"
                     /locus_tag="ACHJZ2_001400"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO4567546.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4970..5464)
                     /locus_tag="ACHJZ2_001401"
     CDS             complement(4970..5464)
                     /locus_tag="ACHJZ2_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO4567547.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5547..6002)
                     /gene="yfbV"
                     /locus_tag="ACHJZ2_001402"
     CDS             complement(5547..6002)
                     /gene="yfbV"
                     /locus_tag="ACHJZ2_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO4567548.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6340..7542
                     /gene="ackA"
                     /locus_tag="ACHJZ2_001403"
     CDS             6340..7542
                     /gene="ackA"
                     /locus_tag="ACHJZ2_001403"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO4567549.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7617..9761
                     /gene="pta"
                     /locus_tag="ACHJZ2_001404"
     CDS             7617..9761
                     /gene="pta"
                     /locus_tag="ACHJZ2_001404"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4567550.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9951..11471
                     /gene="yfcC"
                     /locus_tag="ACHJZ2_001405"
     CDS             9951..11471
                     /gene="yfcC"
                     /locus_tag="ACHJZ2_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO4567551.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11504..12046)
                     /gene="yfcD"
                     /locus_tag="ACHJZ2_001406"
     CDS             complement(11504..12046)
                     /gene="yfcD"
                     /locus_tag="ACHJZ2_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO4567552.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12104..12655)
                     /gene="yfcE"
                     /locus_tag="ACHJZ2_001407"
     CDS             complement(12104..12655)
                     /gene="yfcE"
                     /locus_tag="ACHJZ2_001407"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO4567553.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12711..13355)
                     /gene="yfcF"
                     /locus_tag="ACHJZ2_001408"
     CDS             complement(12711..13355)
                     /gene="yfcF"
                     /locus_tag="ACHJZ2_001408"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO4567554.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13491..14138
                     /gene="yfcG"
                     /locus_tag="ACHJZ2_001409"
     CDS             13491..14138
                     /gene="yfcG"
                     /locus_tag="ACHJZ2_001409"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO4567555.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14195..14557
                     /gene="folX"
                     /locus_tag="ACHJZ2_001410"
     CDS             14195..14557
                     /gene="folX"
                     /locus_tag="ACHJZ2_001410"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO4567556.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14578..15471
                     /locus_tag="ACHJZ2_001411"
     CDS             14578..15471
                     /locus_tag="ACHJZ2_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO4567557.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15519..16409)
                     /gene="rpnB"
                     /locus_tag="ACHJZ2_001412"
     CDS             complement(15519..16409)
                     /gene="rpnB"
                     /locus_tag="ACHJZ2_001412"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO4567558.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16606..17379)
                     /gene="hisP"
                     /locus_tag="ACHJZ2_001413"
     CDS             complement(16606..17379)
                     /gene="hisP"
                     /locus_tag="ACHJZ2_001413"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO4567559.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17387..18103)
                     /locus_tag="ACHJZ2_001414"
     CDS             complement(17387..18103)
                     /locus_tag="ACHJZ2_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4567560.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18100..18786)
                     /gene="hisQ"
                     /locus_tag="ACHJZ2_001415"
     CDS             complement(18100..18786)
                     /gene="hisQ"
                     /locus_tag="ACHJZ2_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO4567561.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18876..19658)
                     /gene="hisJ"
                     /locus_tag="ACHJZ2_001416"
     CDS             complement(18876..19658)
                     /gene="hisJ"
                     /locus_tag="ACHJZ2_001416"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO4567562.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19879..20661)
                     /gene="argT"
                     /locus_tag="ACHJZ2_001417"
     CDS             complement(19879..20661)
                     /gene="argT"
                     /locus_tag="ACHJZ2_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO4567563.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20927..21496)
                     /gene="ubiX"
                     /locus_tag="ACHJZ2_001418"
     CDS             complement(20927..21496)
                     /gene="ubiX"
                     /locus_tag="ACHJZ2_001418"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO4567564.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21591..23108)
                     /gene="purF"
                     /locus_tag="ACHJZ2_001419"
     CDS             complement(21591..23108)
                     /gene="purF"
                     /locus_tag="ACHJZ2_001419"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO4567565.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23145..23633)
                     /gene="cvpA"
                     /locus_tag="ACHJZ2_001420"
     CDS             complement(23145..23633)
                     /gene="cvpA"
                     /locus_tag="ACHJZ2_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO4567566.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(24165..24827)
                     /gene="dedD"
                     /locus_tag="ACHJZ2_001421"
     CDS             complement(24165..24827)
                     /gene="dedD"
                     /locus_tag="ACHJZ2_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO4567567.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24817..26085)
                     /gene="folC"
                     /locus_tag="ACHJZ2_001422"
     CDS             complement(24817..26085)
                     /gene="folC"
                     /locus_tag="ACHJZ2_001422"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO4567568.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(26155..27069)
                     /gene="accD"
                     /locus_tag="ACHJZ2_001423"
     CDS             complement(26155..27069)
                     /gene="accD"
                     /locus_tag="ACHJZ2_001423"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO4567569.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(27225..27884)
                     /locus_tag="ACHJZ2_001424"
     CDS             complement(27225..27884)
                     /locus_tag="ACHJZ2_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4567570.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27967..28779)
                     /gene="truA"
                     /locus_tag="ACHJZ2_001425"
     CDS             complement(27967..28779)
                     /gene="truA"
                     /locus_tag="ACHJZ2_001425"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO4567571.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28779..29792)
                     /locus_tag="ACHJZ2_001426"
     CDS             complement(28779..29792)
                     /locus_tag="ACHJZ2_001426"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4567572.1"
                     /translation="MSEGWNIAVLGTTGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29858..30994)
                     /gene="pdxB"
                     /locus_tag="ACHJZ2_001427"
     CDS             complement(29858..30994)
                     /gene="pdxB"
                     /locus_tag="ACHJZ2_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO4567573.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            31093..32088
                     /gene="flk"
                     /locus_tag="ACHJZ2_001428"
     CDS             31093..32088
                     /gene="flk"
                     /locus_tag="ACHJZ2_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO4567574.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHCPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(32085..33263)
                     /locus_tag="ACHJZ2_001429"
     CDS             complement(32085..33263)
                     /locus_tag="ACHJZ2_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4567575.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33538..34758)
                     /gene="fabB"
                     /locus_tag="ACHJZ2_001430"
     CDS             complement(33538..34758)
                     /gene="fabB"
                     /locus_tag="ACHJZ2_001430"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO4567576.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34917..36923
                     /gene="mnmC"
                     /locus_tag="ACHJZ2_001431"
     CDS             34917..36923
                     /gene="mnmC"
                     /locus_tag="ACHJZ2_001431"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO4567577.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(37044..37322)
                     /locus_tag="ACHJZ2_001432"
     CDS             complement(37044..37322)
                     /locus_tag="ACHJZ2_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO4567578.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37356..37904)
                     /gene="epmC"
                     /locus_tag="ACHJZ2_001433"
     CDS             complement(37356..37904)
                     /gene="epmC"
                     /locus_tag="ACHJZ2_001433"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO4567579.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37904..38713)
                     /locus_tag="ACHJZ2_001434"
     CDS             complement(37904..38713)
                     /locus_tag="ACHJZ2_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO4567580.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38713..39537)
                     /gene="mepA"
                     /locus_tag="ACHJZ2_001435"
     CDS             complement(38713..39537)
                     /gene="mepA"
                     /locus_tag="ACHJZ2_001435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO4567581.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39541..40626)
                     /gene="aroC"
                     /locus_tag="ACHJZ2_001436"
     CDS             complement(39541..40626)
                     /gene="aroC"
                     /locus_tag="ACHJZ2_001436"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO4567582.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40661..41593)
                     /gene="prmB"
                     /locus_tag="ACHJZ2_001437"
     CDS             complement(40661..41593)
                     /gene="prmB"
                     /locus_tag="ACHJZ2_001437"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4567583.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            41759..42310
                     /gene="smrB"
                     /locus_tag="ACHJZ2_001438"
     CDS             41759..42310
                     /gene="smrB"
                     /locus_tag="ACHJZ2_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO4567584.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42481..43323)
                     /locus_tag="ACHJZ2_001439"
     CDS             complement(42481..43323)
                     /locus_tag="ACHJZ2_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO4567585.1"
                     /translation="MKKWTIFLTSLILLALSLETPKCYAGDKLMSASFSSTKIYYAMK
                     NVTASGSLYFYVTVVTPGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTD
                     TSNCPGINTRVFVCAYMTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSIC
                     PTIPVASFDISWSDNYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYV
                     LNEL"
     gene            complement(43325..43846)
                     /locus_tag="ACHJZ2_001440"
     CDS             complement(43325..43846)
                     /locus_tag="ACHJZ2_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4567586.1"
                     /translation="MKNNRAWALISGLILFSGTAPAADNLHFTGNLLGKSCTPVINGN
                     LLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKVTLMGTEDTDLPGFLS
                     IDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNFNAWLQTVNGRNVTSG
                     DFTATMTVTFEYF"
     gene            complement(43843..44313)
                     /locus_tag="ACHJZ2_001441"
     CDS             complement(43843..44313)
                     /locus_tag="ACHJZ2_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4567587.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVPYTITCDTAVRDPQMEMTLTWSGTQSDFDDSAVATDLNGLG
                     IHLKQAGSDFKLHTPLVVNETSLPVLTAVPVKKSGVDLPESDFEAWATLQVDYQ"
     gene            complement(44310..44810)
                     /locus_tag="ACHJZ2_001442"
     CDS             complement(44310..44810)
                     /locus_tag="ACHJZ2_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4567588.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHFILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            complement(44821..45579)
                     /locus_tag="ACHJZ2_001443"
     CDS             complement(44821..45579)
                     /locus_tag="ACHJZ2_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4567589.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            complement(45602..48241)
                     /locus_tag="ACHJZ2_001444"
     CDS             complement(45602..48241)
                     /locus_tag="ACHJZ2_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4567590.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKKLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            complement(48323..48886)
                     /locus_tag="ACHJZ2_001445"
     CDS             complement(48323..48886)
                     /locus_tag="ACHJZ2_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4567591.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12022 a        13179 c        12184 g        11526 t
ORIGIN      
        1 tttgaatatt aaataataat taattagcat catcctcatt cattaattcc gcttaacaat
       61 agtttcacac cttgccccca ttgcgacacc tcggaaatca tcacgctgtt aaactctccg
      121 taatgcggaa atatcatcat cgcgtttcat tagtgaattc ttaaatgagg cattttcaca
      181 caattatctt acagataaaa aaaccagact tacaattaag aatcagaaca attcactata
      241 taacattgca tgtaaagcat atgcacctca ttattttgtc attattaagt tattaacagc
      301 acaatcgcgc cttcccctct ggcaaaatct tattctgcag accttcaaaa caccgtcctg
      361 ggggagtaca ttgttctaag ctgacttcca cggcagggag tggcgataac agcaaaaaag
      421 gtcaagattc atgtccccca ttgaaaaatc cagcaaatta gagaatgtct gttatgacat
      481 ccgtggtccg gtgctgaaag aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa
      541 actgaacatc ggcaacccag ccccgttcgg ttttgacgcg ccagatgaaa tcctcgttga
      601 cgtgatacgc aacctgccta ccgctcaagg gtattgcgat tccaaaggtc tttactccgc
      661 gcgtaaagcc atcatgcagc actatcaggc tcgtggcatg cgtgatgtta ccgtggaaga
      721 tatttacatc ggcaatggtg tatcggagct tatcgttcag gcaatgcagg cattgctgaa
      781 cagcggggac gaaatgttgg ttcctgcacc agattaccca ctatggaccg cggcggtttc
      841 gctttccagc ggtaaagcgg tgcattatct ttgcgatgaa tcctctgact ggttcccgga
      901 cctcgatgat attcgcgcta aaattacgcc tcgtacgcgt gggatcgtta ttatcaaccc
      961 aaataaccca accggcgcgg tatattccaa agagctttta atggagattg tggagattgc
     1021 acgtcagcat aatctcatta tcttcgccga tgaaatttat gacaaaattc tctacgacga
     1081 cgctgagcat cactcaattg cgccgctggc acctgacctg ctgaccatta cttttaatgg
     1141 actgtcgaaa acgtaccgcg ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc
     1201 gaaaaaacac gccaaaggct acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg
     1261 tgctaacgtt cctgcgcaac acgccattca gaccgcgctg ggtggttatc agagcatcag
     1321 tgaatttatt acccctggcg gtcgtcttta tgagcagcgt aaccgcgcgt gggaattgat
     1381 caacgatatt ccgggcgttt cctgcgtgaa accttgtggt gcgctgtaca tgttcccgaa
     1441 aatcgacgcc aaacgcttta acattcacga cgatcagaaa atggtgctgg atttcctgtt
     1501 gcaggaaaaa gttctgttgg tgcaagggac ggcattcaac tggccgtggc cggatcactt
     1561 ccgcattgtc acgctaccgc gtgtcgatga tatcgagctg tcgttgagca agttcgcgcg
     1621 tttcctttct ggttatcatc agctgtaatc ttaatttcac tgccggagat tgcatccggc
     1681 agtgttatcc cgccacaatg acctgatgat gtcatcatac gtaaggtcac tatgaaacag
     1741 agccattttt ttgcccatct ctcccgcctg aaactcatta accgctggcc gctcatgcgc
     1801 aacgtgcgga cggaaaatgt gtccgaacac agtttgcagg tagcgatggt cgcccatgcg
     1861 ctggcagcta tcaaaaatcg gaaatttggc ggtaatgtca acgccgaacg tatcgcttta
     1921 ctggcgatgt accacgatgc ctcagaagtg ctcaccggcg atctccctac tccggtgaaa
     1981 tacttcaatt cgcaaatcgc tcaggagtac aaggctattg aaaaaatcgc tcagcaaaaa
     2041 ctggtcgata tggttccgga agagctgcgg gatatctttg cgccgttaat tgacgagcat
     2101 gcatatagcg atgaagaaaa atcgctggtg aaacaggcag atgcactgtg tgcatatctg
     2161 aaatgtctgg aagaactcgc ggcgggaaat aatgaattct tgctggcaaa aacgcgactg
     2221 gaagcgacgc ttgaagcgcg ccgcagccag gagatggact acttcatgga agtatttgtt
     2281 cccagcttcc atctttcgct cgatgagatt agccaggatt caccgctgta agcagctgga
     2341 gtctgcgtcg catcaggcaa taagcgccgg atgcgacatc aggctcttgt caaaacggaa
     2401 acagcatcgg gatcatcacc acacaaaccg ccatcacgat aatggtgaac ggtaccccca
     2461 gcttcacaaa gtcactgaag ctgtaatttc ccggacctaa aaccaatgtg ttaacaggtg
     2521 aagaaaccgg tgtcataaag gcggcggatg ctgccatcgc cacgaccatc gcgaatggat
     2581 aaggcgacac ccccatcgtt ttggcagcag ccagcgcaat cggagccatc aacaccgccg
     2641 tcgcggtatt agagataaat agcccaataa ccgccgacaa gacaaacaaa caacccagca
     2701 tcatatgtgg cccgtaacca ccgccaatgt ccatcagccc tttcaccgcc agcgcgacac
     2761 ctcccgtttt ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa ataatgctcg
     2821 gccagtgaat ggatttatag gcgctttcag catctataca gcggaatttc cccatcagca
     2881 ggcaggcgat gatagcggca acaggattag gaatttcatc tgtcagcatt aacgccacca
     2941 ttagcaccag acagaaaatg gcatggggtg cctggctgtg cgcgggcgat gcttcactca
     3001 cctcttccgg taagttcagc gctacgaagt cgcggccctg tttggccagc ataccgatca
     3061 gtttccagtt acccacaacc aggatgatat cgcccagcag cagaggctca tccgccagcg
     3121 aaccttccag cgccacgcca ttgcgcttta gccccaccac attcagtccg tagcgggtac
     3181 gaaaaccaat ttcgcgcacc gatttaccaa tcagttctga ctcaggaatt aatgaaatct
     3241 ctgccatgcc cacatcaagg gcctggtcag aaaaatactc gccgcgcagt accatcggct
     3301 ccagcaattg ctcactacaa aattgccgga gatcgacatc agccgcagac atatcaataa
     3361 gcaaaacgtc acgcgcgcga aattcagaaa ccccattaac gttcacgata acgcgacgaa
     3421 aacgccgcca gcgttcaaca ccgatgacgt tagcgccata acgctcacgt aatttgagat
     3481 catccagccg ttgaccaatc attggcgatc cggggcgaat agccagacgt cgcgcacgcc
     3541 cggtcagtcg atattcacgg ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct
     3601 gcggggtctg ggtatcccct ttcagcatga aacgcatcac taacatatac aaaataccca
     3661 gcaccagcac gaccaggcca ataggtgtta cgctaaagaa actgaagccg tgatagcctt
     3721 cacgcagcaa ttcactgttg actaccaggt tcggcggcgt cgccaccagc gtcatcatgc
     3781 cgctaatcag cccggcaaaa cttaacggca tcatcagacg tgacggcgaa gtttgcatac
     3841 gcatggcaac gcttaacacc acgggaataa agatagcgac aacgccggtt gaactcataa
     3901 acgcgccaag ccccgcgacg gtcagcatca acaaaaccaa cattttgatt tcactgttgc
     3961 ccgcaacttt gaccagccat gttcccatta cggtggcaac accggtacgg accaaaccat
     4021 cgccaataat aaacaaggcg gcaatcagga caacgttagg atcagaaaag ccagaaaata
     4081 cttctgggac tgtcagcgtt ccgcttaatg caaacgcgac aataacaaac aaagcgaccg
     4141 catccatacg cactctgccc gtcgcaaaca agacgatggc aacggccagt aatgaaagaa
     4201 cccaaatcaa ttcaccgttc acaacttatc cttgttaatt gagggggatg acttgattct
     4261 gccataaaaa agccccgacg agacggggct aaatcatgat caggtgtttc actgaataat
     4321 aacatcgcca tttggctgtt tggtcacagt aatttgctcc agactgtgga ggaccaaatc
     4381 gacctcattc aggcgcgggg tatctgccgg agcgttaacc gcaatgacat ggcaacccgc
     4441 cgccaggcca gaaagcacgc cagcgggagc atcttccacc accacacact cctgcggtgc
     4501 aagccccagc agctgcgcgc ctaacagata cgcatcaggt tctggttttc cgcgcttcac
     4561 tcgctcagcg gttacaaaca cctctggtgc gggaagcccc gctattttat ggcgcgctcg
     4621 cgctaccggc atggagccag aagtcacaat ggcccacgga atacctgctt tattcaaatg
     4681 attgagtaag gcgattgccc ccggaagcgc ggtaatacct tcggtttccg tggcctcgat
     4741 ttgctccaaa cgcgtaaact cggcggcaat atcagcctcg gatttgcccg ccataaaatg
     4801 gcgcagagag gtgatcgcct gtttaccgtg aatgaaagcc agcacctctt ccggcgctaa
     4861 cccatgacgt ctggcccagt tgctccacgc ccgttctacc gcaggcaggg aatccaccag
     4921 cgttccatca agatcaaaca gaaaaccttt gcaccgcacg cgggcctcct caggcgttga
     4981 taatttgatt aatttcgttg gcgctcaaat ggtactgacg cgggcaggca tgccacacat
     5041 taagcatgcg ctgatatttt tcccacattg gcgtctgggc gttaaaaccg tgagttccgg
     5101 cgtcaaaatg ggtatagcgc ccttccacat taaccataaa gcggacataa ccgaggtaac
     5161 gtgcttcagt ggcggcgtca aagccgagga aggtgacacg acgttcatcg atggattgct
     5221 ggtcctgcaa attagaccag gaaacatgca acgcatgata catctccata atgtcgatga
     5281 tagtgcggca ggtttcttct ttcagctcgc caaactcgcg atccagttca cgcatctgta
     5341 atccataacc ccgctcaatg attgtttgca ggcgacggta acgttcagca tttgccggat
     5401 cgagcatagt catcatcttg tactggttag acaaaataag acgttgcgcg ttggtcattt
     5461 ccattgttga ctcctgtatc actctactac ggtgaaaaaa aagaaggctg agtatgcctt
     5521 cttttatatg cgtaatcagg ggtcaattac aaatcatcaa ggaaagtttt atccagttgt
     5581 ttgaaggcac gcttaagcgt gtcagctaat gcctggtaat caggcttgcc ttcaacgggt
     5641 gccaacacct gtccagactc ctgcaattta ccgcgaactt cataaaacca gttgaggatt
     5701 gcagggggta atggcgtgac agaacgcttg cccagccacc acaatccctg catgggtaaa
     5761 cttaaggcga acagggcagt ggcaactgcc ggcccaagct gaccgcccag ggcaatctgc
     5821 cagcagagag taaatacggc gatcggcggc ataaaacgga tcgcataacg cgtcatcttg
     5881 ataacgcgat tttcgacaaa gaccggggca aggcgttttt ccagcggcca cgtctttgag
     5941 taatgctgtc cccggcgaaa caagctaaaa aaattaacag aacgattatc cggcgttgac
     6001 atgcttcacc tcaacttcac atataaagat tcaaaaattt gtgcaaattc acaactcagc
     6061 gggacaacgt tcaaaacatt ttgtcttcca tacccactat caggtatcct ttagcagcct
     6121 gaaggcctaa gtagtacata ttcattgagt cgtcaaattc atatacatta tgccattggc
     6181 tgaaaattac gcaaaatggc atagactcaa gatatttctt ccatcatgca aaaaaaattt
     6241 gcagtgcatg atgttaatca taaatgtcgg tgtcatcatg cgctacgctc tatggctccc
     6301 tgacgttttt ttagccacgt atcaattata ggtacttcca tgtcgagtaa gttagtactg
     6361 gttctgaact gcggtagttc ttcactgaaa tttgccatca tcgatgcagt aaatggtgaa
     6421 gagtaccttt ctggtttagc cgaatgtttc cacctgcccg aagcacgtat caaatggaaa
     6481 atggacggca ataaacagga agcggcttta ggtgcaggcg ccgctcacag cgaagcgctc
     6541 aactttatcg ttaatactat tctggcacaa aaaccagaac tgtctgcgca gctgactgct
     6601 atcggtcacc gtatcgtaca cggcggcgaa aagtatacca gctccgtagt gatcgatgag
     6661 tctgttattc agggtatcaa agatgcagct tcttttgcac cgctgcacaa cccggctcac
     6721 ctgatcggta tcgaagaagc tctgaaatct ttcccacagc tgaaagacaa aaacgttgct
     6781 gtatttgaca ccgcgttcca ccagactatg ccggaagagt cttacctcta cgccctgccg
     6841 tacaacctgt acaaagagca cggcatccgt cgttacggcg cgcacggcac cagccacttc
     6901 tatgtaaccc aggaagcggc aaaaatgctg aacaaaccgg tagaagaact gaacatcatc
     6961 acctgccacc tgggcaacgg tggttccgtt tctgctatcc gcaacggtaa atgcgttgac
     7021 acctctatgg gcctgacccc gctggaaggt ctggtcatgg gtacccgttc tggtgatatc
     7081 gatccggcga tcatcttcca cctgcacgac accctgggca tgagcgttga cgcaatcaac
     7141 aaactgctga ccaaagagtc tggcctgctg ggtctgaccg aagtgaccag cgactgccgc
     7201 tatgttgaag acaactacgc gacgaaagaa gacgcgaagc gcgcaatgga cgtttactgc
     7261 caccgcctgg cgaaatacat cggtgcctac actgcgctga tggatggtcg tctggacgct
     7321 gttgtattca ccggtggtat cggtgaaaat gccgcgatgg tccgtgaact gtctctgggc
     7381 aaactgggcg tgctgggctt tgaagttgat catgaacgca atctggctgc acgtttcggc
     7441 aaatctggtt tcatcaacaa agaaggtacc cgtcctgcgg tggttatccc aaccaacgaa
     7501 gaactggtta tcgcgcaaga cgcgagccgc ctgactgcct gatttcacac cgccagctca
     7561 gctggcggtg ctgttttgta acccgccaaa tcggcggtaa cgaaagagga taaaccgtgt
     7621 cccgtattat tatgctgatc cctaccggaa ccagcgtcgg tctgaccagc gtcagccttg
     7681 gcgtgatccg tgcaatggaa cgcaaaggcg ttcgtctgag cgttttcaaa cctatcgctc
     7741 agccgcgtac cggtggcgat gcgcccgatc agactacgac tatcgtgcgt gcgaactctt
     7801 ccaccacgac ggccgctgaa ccgctgaaaa tgagctacgt tgaaggtctg ctttccagca
     7861 atcagaaaga tgtgctgatg gaagagatcg tcgcaaacta ccacgctaac accaaagacg
     7921 ctgaagtcgt tctggttgaa ggtctggtcc cgacacgtaa gcaccaattt gcccagtctc
     7981 tgaactacga aatcgctaaa acgctgaatg cggaaatcgt cttcgttatg tctcagggca
     8041 ctgacacccc ggaacagctg aaagagcgta tcgaactgac ccgcaacagc ttcggcggtg
     8101 ccaaaaacac caacatcacc ggcgttatcg ttaacaaact gaacgcaccg gttgatgaac
     8161 agggtcgtac tcgcccggat ctgtccgaga ttttcgacga ctcttccaaa gctaaagtaa
     8221 acaatgttga tccggcgaag ctgcaagaat ccagcccgct gccggttctc ggcgctgtgc
     8281 cgtggagctt tgacctgatc gcgactcgtg cgatcgatat ggctcgccac ctgaatgcga
     8341 ccatcatcaa cgaaggcgac atcaatactc gccgcgttaa atccgtcact ttctgcgcac
     8401 gcagcattcc gcacatgctg gagcacttcc gtgccggttc tctgctggtg acttccgcag
     8461 accgtcctga cgtgctggtt gccgcttgcc tggctgccat gaacggcgta gaaatcggtg
     8521 ccctgctgct gactggcggc tacgaaatgg acgcgcgcat ttctaaactg tgcgaacgtg
     8581 ctttcgctac cggcctgccg gtatttatgg tgaacaccaa cacctggcag acttctctga
     8641 gcctgcagag cttcaacctg gaagttccag ttgacgatca cgagcgtatc gagaaagttc
     8701 aggaatacgt tgctaactac atcaacgctg actggatcga ttctctgact gccacttctg
     8761 agcgcagccg tcgtctgtct ccgccagcgt tccgttatca gctgactgaa cttgcgcgca
     8821 aagcgggcaa acgtatcgta ctgccggaag gtgacgaacc gcgtaccgtt aaagcagccg
     8881 ctatctgtgc tgaacgtggt atcgcaactt gcgtactgct gggtaatccg gcagagatca
     8941 accgtgttgc agcgtctcag ggtgtagaac tgggtgcagg cattgaaatc gttgatccag
     9001 aagtggttcg cgaaagctat gttggtcgtc tggtcgagct gcgtaagaac aaaggcatga
     9061 ccgaaaccgt tgcccgcgaa cagctggaag acaacgtggt gctcggtacg ctgatgctgg
     9121 aacaggatga agttgatggt ctggtttccg gtgctgttca cactaccgca aacaccatcc
     9181 gtccgccgct gcagctgatc aaaactgcac cgggcagctc cctggtatct tccgtgttct
     9241 tcatgctgct gccggaacag gtttacgttt acggtgactg tgcgatcaac ccggatccga
     9301 ccgcagaaca gctggcagaa atcgcgattc agtccgctga ttccgctgca gccttcggta
     9361 tcgaaccgcg cgttgctatg ctctcctact ctaccggtac ttctggtgca ggtagcgacg
     9421 tagaaaaagt tcgcgaagca actcgtctgg cgcaggaaaa acgtcctgac ctgatgatcg
     9481 acggtccgct gcagtacgac gctgcggtaa tggctgacgt tgcgaaatcc aaagcgccga
     9541 actctccggt tgcaggtcgc gctaccgtgt tcatcttccc ggatctgaac accggtaaca
     9601 ccacctacaa agcggtacag cgttctgccg acctgatctc catcgggccg atgctgcagg
     9661 gtatgcgcaa gccggttaac gacctgtccc gtggcgcact ggttgacgat atcgtctaca
     9721 ccatcgcgct gactgcgatt cagtctgcac agcagcagta acctcgtcat catccgcagc
     9781 tttgcgctgc ggatatctga accggaaata gtcactattt ccggtttttt attctcttaa
     9841 tttgcattaa tcctttctga ttatcttgct taactgcgct gcatcaatga attgcgccat
     9901 ctcactttgc atacttacca ctttgttttg tgcaagggaa tatttgcgct atgtccgcaa
     9961 tcactgaatc caaaccaaca agaagatggg caatgcccga tacgttggtg attatctttt
    10021 ttgttgctat tttaaccagc cttgccacct gggtagttcc ggtggggatg tttgacagtc
    10081 aggaagtgca gtatcaggtt gatggtcaaa caaaaacacg caaagtcgta gatccacact
    10141 catttcgcct tctgactaac gaagcaggcg aacctgagta tcaccgcgta cagctgttca
    10201 cgacgggcga tgaacgcccg ggcctgatga acttcccgtt tgaagggtta acctcaggat
    10261 cgaaatacgg gacagccgtt ggcatcatca tgtttatgct ggtgattggc ggcgcgtttg
    10321 gcattgtgat gcgtacagga accattgata acggtatcct ggcgcttatt cgccataccc
    10381 gtgggaatga aattctcttt attcctgcgc tgtttattct gttttcactt ggcggcgcgg
    10441 tatttggtat gggggaagag gccgtcgcct ttgccattat catcgcaccg ctaatggtcc
    10501 ggctgggcta tgacagtatt accaccgtcc tggtgaccta tattgccacg caaatcggtt
    10561 ttgccagttc gtggatgaac ccgttttgtg tggtcgttgc tcaggggatt gccggcgttc
    10621 cggtgctttc tggctccggg ttgcgcatcg tggtgtgggt tatcgccact ctgattggcc
    10681 tgatctttac catggtgtac gcctcacgag tgaaaaagaa tcctcttctg tcacgcgtgc
    10741 atgagtccga ccgcttcttt cgtgaaaagc aggcggatgt tgaacaacgt ccgtttacct
    10801 ttggtgactg gctggtattg attgtcctga ccgccgtaat ggtctgggtg atttggggcg
    10861 tgatcgttaa tgcctggttt attccagaaa ttgccagcca gttcttcacc atgggtctgg
    10921 tgattggcat catcggcgtt gttttccgcc ttaacggtat gacggttaat accatggctt
    10981 catccttcac cgaaggggcg cgaatgatga tcgcccctgc cctgctggtg ggtttcgcca
    11041 aagggatttt gctgctggtc ggtaatggtg aagcgggtga tgccagcgtg ttaaatacta
    11101 tcctcaacag cattgccaat gccattagcg gtctggacaa cgcggtcgcg gcctggttta
    11161 tgttgctctt ccaggcggta tttaatttct tcgtgacgtc cggttctggt caggcggcgt
    11221 taaccatgcc gttactggca ccgcttggcg atctggtcgg tgttaaccgt caggttaccg
    11281 tgctggcttt ccagtttggt gatggcttca gtcacatcat ttacccgacc tcagcttcgt
    11341 taatggcgac gctcggtgtt tgccgggtgg acttccgtaa ctggctgaag gtgggcgcga
    11401 ccctgcttgg actgctgttt attatgtcca gcgtcgtggt gatcggcgct cagttgatgg
    11461 gctaccacta aaaatgttaa gagccgcaat tgcggctctt ttttcattct gccgtttcag
    11521 tctctacggc ttcatttttg gcattgcgct tcatccacaa cgctagcgct ttcagcgagt
    11581 ctggagtgaa ctcatcgcag cgtgcggtga tttcttccgg cgtcagccag caaacttcac
    11641 tgacttcatc ttcctgtagg gcgaagggac cgtgagagac gcagctgaac aatgcgcccc
    11701 agacacggca atttttatct tcgaaataga actgcccgtg ctcggcaaag gggacaccgg
    11761 caatgcccaa ctcttcttcc gcttcgcgac gcgcggattc cagcagttgc tcatcggcct
    11821 ggactacacc gccagcggtc gcatctaaca tgccgggtaa aaagtctttt gtctcggtac
    11881 gacgctggac cagaattttg cccatgccat catgcacgac gatgtaagtt gcacgatgac
    11941 gcagacactg tgcccgcatt tgctcccggc tggcttgtgc aatgacttcg ttctcttcat
    12001 tgacaatatc cacccattca gtacttgcca aacgacgctg ttccattatc aggaaacctt
    12061 ctttttctgg cgctcttacg gcgcattttg agttgtgggt aaattacgga ttaatcgcga
    12121 cctgcgcaat gatactttga tcattgagtg cgattacact aagtacgtca ttatccagca
    12181 tgccataact cgccggatta ccgcctttcg gaatactcac cgagccgggg ttgaagtgga
    12241 aaatctcccc ccgctgttct gctactggca gatgggtatg accgtaaacc agcacatcgt
    12301 tctggtttaa agcaggcaga ttttccgggc caaaaagatg accatgcgtc aaaaacagac
    12361 gttgtttttc cagtaatacc tgttgccacg gcgcggttat cgggaaatgc agcagcattt
    12421 gatccacttc gctgtcgcag ttgccgcgca cagcgataac cttatgtgcc acttcattaa
    12481 gccgttcagc gactttggct ggcgcgtaac cctccggtaa agcattacgc gggccgtgat
    12541 tcaacacgtc gccaaggatc accagccact gggcaccgct ttgggcaaac aactccagaa
    12601 cacgttccgt cgccggtaac gacccatgaa tgtccgatgc aaacatcagt ttcatcactc
    12661 actcctcgtc gaaaaaacgt aagctatgat actggattct gtcgttgcta tcagccagat
    12721 tgcttcgctg aaagtgcaat aaaacgctgg acagacgctc gctgccactg gaatgttgca
    12781 taatccacca ggcattctgg cacctcatcg ccatgtagta ccaggcggtt aatcattagc
    12841 gccagatcag tatcagcaat gcaccattca ccaaataaat tcggctgacc cagtactaac
    12901 aaatgttctg ccatcgcgaa cagtttctct gcactggctt ttccctcggc cgttagtggc
    12961 gcttttttcg cccccgcaaa gacaacatct gtcggacgct cttcgcggat gggcatcaga
    13021 tcgctgcgca accaggcctg aatctgtcgt gcacgcgcac gattttctaa atcaagcgga
    13081 taaatacgtt cccaggtcgg tggcgcaaat cgatcttcca gatactccgc aatggcagaa
    13141 gattcactca actcaaaatc atcgatttgt aataacggca cacggcgtgt ctgaccgtaa
    13201 ccttgccacg tcggttgcaa atgttcaccg ctgtcgaggt cgatggtctt gatatgaaac
    13261 aacaggcctt tttcctgcaa cgccacccaa gcggataaca cataagggga gaaaaaatgg
    13321 gcatctgacc aaagcgtgat agcgggttta ctcattatgt cctcggcaat aaagggggct
    13381 ggctatcaaa ctatagagcc tttttgtcac tgtcacgtga cgaatactca cgtaagtcgc
    13441 cgcgctttct ataattgtgg tgttcagatg attagacaac cggagctcca atgatcgatc
    13501 tctatttcgc cccgacaccc aatggccaca aaattacgct gtttctcgaa gaagcagggc
    13561 tggattatcg cttgataaag gtagacctgg ggaaaggcgg tcagtttcgc ccggaatttt
    13621 tgctcatttc gcctaacaac aaaattccgg caattgttga tcattctcca gccgatggcg
    13681 gcgaaccgct aagcctcttt gaatctggtg ccattttgtt gtatctggct gagaaaacgg
    13741 ggctcttttt gagtcatgaa acgcgtgaac gcgccgccac attacagtgg ttattctggc
    13801 aggtaggcgg actggggccg atgcttgggc aaaatcatca ttttaatcac gcagcccccc
    13861 aaaccattcc ttacgctatt gaacgttatc aggttgaaac tcagcgtctg taccatgtac
    13921 tgaacaagcg gctggaaaac tcgccctggc tgggaggcga gaactacagc attgcggata
    13981 ttgcctgctg gccgtgggtt aatgcctgga ctcgccagcg aatagacctc gctatgtatc
    14041 cggcagtcaa gaactggcat gagcggatcc gttcgcgccc tgccaccggg caggcactgc
    14101 taaaagcaca actcggtgat gagcgttcgg atagttaaca gaaacaggtt ctcgtgtatt
    14161 atttcatcct aagtaaaaca acggagaacc tgcaatggca caacctgccg ctattattcg
    14221 tataaagaac cttcgtttac gtacatttat cggaattaag gaagaagaaa ttaacaaccg
    14281 tcaggatatt gttatcaatg tgacgatcca ctaccccgcc gataaagcgc gcactagcga
    14341 agatatcaac gatgcgctga attatcgcac cgtaacgaaa aacattattc agcatgtaga
    14401 gaataaccgt ttctctttgc tggaaaaatt aactcaggat gtgctcgata tcgcacgtga
    14461 acatcactgg gtgacgtatg ctgaagtgga gatcgataaa ctgcacgcgc tgcgctacgc
    14521 cgattcggta tccatgacct taagctggca gcgttaaccg ccatatcggg aggctgcatg
    14581 aacatagtga tcaccggagg gacgggatta attggtcgcc atttgattcc acgtttgctg
    14641 gagctgggcc atcaaatcac ggtaatgacg cgtaacccgc agaaagccag ttccgttctc
    14701 ggccctcggg tgacactatg gcaagggctt gccgatcaaa gcaacctcaa cggcattgat
    14761 gcggtaatca acctggccgg agaaccgatt gctgataaac gttggactca cgagcaaaaa
    14821 gagcgtctct gccaaagccg ctggaatatc acgcaaaaac tggtcgattt gattaatgcc
    14881 agcgacacgc caccgtcggt actcatttcc ggctcggcaa cgggctatta tggcgactta
    14941 ggtgaagtgg tggttaccga agaggaaccg ccgcataaca aatttaccca taaactctgc
    15001 gcccgctggg aagaaattgc ctgtcgggcg caaagtgaca aaacgcgagt gtgcctgctg
    15061 cgtaccggcg tagtgctggc accggatggc ggtattctcg gtaaaatgct gccgccgttt
    15121 cgtcttggcc tgggcgggcc gattggttcc ggtcggcagt atctggcctg gattcatatc
    15181 gatgatatgg tcaacggcat tctctggctg ctggataacg agctgcgcgg gccatttaat
    15241 atggtttcgc cctacccgat acgcaatgaa caatttgccc atgcgctcgg tcatgcgctg
    15301 catcgcccgg ctattttgcg cgtccccgca accgccattc ggctgttaat gggcgaatct
    15361 tcagtactgg tattaggtgg acaacgcgcg ctgcctaaac ggctggaaga agcgggtttt
    15421 gcgtttcgct ggtacgattt agaagaggcg ctggcggatg tcgttcgctg atgtggttta
    15481 ctgcaaacat ccgccagtta actcccggtg ttacagggtt agtggctttg cgcgataaga
    15541 tcgtctggtg aaagtcgggt cagcgtcagg atgacttcgt ggtcaaatcc tttttccagc
    15601 atctcctgag caatacgcag agcttcttcg tgtttgccct gcattgcgcc ttcttcacgt
    15661 aatctgtcag caatggtcat cagtttctcc ttttcttgtg gtgcgcgttc tgttatctca
    15721 ccaataaatg cacggaaacg ctgggcatct cccgtttgta atacataatt aaacagggct
    15781 tttagctgtc tgtcattagt gttccctgta actagcagcg aaacaatttg gtcgactaat
    15841 cccaacagat cgcgctgacg aatatgtttc tgaattaact ccaacagcgc cattttacga
    15901 tgttgcataa tctcgtcatc cggcaccacg gtaatatcca ccaacggaaa agccgatgaa
    15961 tatattttac gggcaatcgc aggctcggca aattcatcaa gccagcagag tgaataagga
    16021 taaggacttc tgcaaccatg ataaaacagc atcgggatca ccaatggaag ctctttatag
    16081 cccacatcaa gatggttttg cattgccgca atggaataac gcatcatgcg aaaagccatt
    16141 aattcttccg gcttactttg gtgctctatc actacataaa gataacccac tccctcctgc
    16201 gttttcacag accacaagag gtcggaataa tattgccgca ggtcttcatc aataaaactg
    16261 tttggttcca gtttaagcgt cgttaaatca cacagtttgc gcagcggcgc gggaagatga
    16321 atatcaataa aatcccgcgc ggtgtctgga tggcgtaaaa aagatttaaa taccgcatca
    16381 tgcggcgtgg aagttgtcga tattgtcatg gcgttccgtc acccctcaaa tttaagatga
    16441 cgcgacaata accacgatta acacccaatg cagcaggcag tttttatctt tggacagcga
    16501 attcagaagt atttttagcg gactgcaact tttacatttc agcggaagct gactcgcaaa
    16561 ttccccttga aaatactccg gcagcgttag ccaccggaga ggcaattatt tcagcgaacc
    16621 tttaaggaac tgttgcagac gagggctttg cgggttgcca aataactgct ccggcgcgcc
    16681 ttcttcctct attttcccct gatggaggaa aatgacgtga gtagatacat ggcgagcaaa
    16741 gcccatttcg tgggtcacca ccaccatcgt ttttccctct tccgccagct gctgcataat
    16801 gcgcagcact tcacccacca gctctgggtc aagggccgat gtcggttcat caaacaacag
    16861 aacttccggt tccattgcca gcgcccgcgc tatggaaaca cgctgttgct gaccaccgga
    16921 caggtgcacc gggtatttcc cctgcgcacg ttcgtctatc ccgacttttg ccagatactt
    16981 caccgcccgc tcgcgcgctt cctgcttgct caggcccaac acctgaatcg gcgcttccat
    17041 gacgttttcc agcaccgtca tatggctcca gagattgaag tgctggaata ccatcgtcag
    17101 gcgtgtgcgc agtaagcgca gttgattttt atcggcgact ttgagttgac cgtctttgtc
    17161 gcgcaccaga ttgatcgtct ggccattgac cacgatcgac ccttcactcg gtttttcgag
    17221 gaagttaatg cagcgcagaa aggtactttt ccccgatccc gacgatccga tgatgcttat
    17281 tacatctccg gcattcgctt gcagtgacac ccctttcagc acttcgtgtt cgccatagcg
    17341 tttgtgcaaa tcgataacgt ttaatttatt ctcggacatc gtattctcag tgcgttgaag
    17401 aaggtttcac atgctgcaac cagcgttttt ccgctctgcg aaagaggctg atcaggacat
    17461 aagagatgat taaatagagc accgcggcaa tgccgaaggc ggtaaaaggt tgatacgtgg
    17521 cggcgttaat atcgcgggct attttcagca gatccggcac cgtggcagta aatgccaacg
    17581 cagtagagtg cagcatcagg atcacttcgt tgctgtatgc cggtaacgca atacgcagcg
    17641 cagaaggcaa aataatgcag cgatacattt taaaagtcga gaagccatag gctctggcgg
    17701 cttcaatttc cccatgcggc accgaacgga ttgccccagc aaaaatctcg gtagtgtaag
    17761 cgcaggtgtt aagcgtcagc gccagcacgg tacagttcag gccactgcgg aaaaaggcgt
    17821 taagaaactc ggtcccctta acaatctcca gcgtatacat gccggaatag aacaccagca
    17881 actgaacata cagcggcgta ccgcgaaaaa tataggtaaa taaccagatt ggaaactgga
    17941 tgtatttatt actggagaca cgaccaatcg ccagaaacag cgccaggact ccgcctatca
    18001 ctaccgacaa aataagcagc cacagagtga tcgccacacc agtaaagcga taaccgtcgg
    18061 tccacagcag cggtttccag tattcatgta agatttcgat cacaggtcag ccctcttcac
    18121 acccacagag tagcggcgct caaggaacag cagcacacca ttggaaacgg tggtgaaaac
    18181 caggtaaatc acgccacaga cgatagcgaa atagaacggt tcccaggtac ttttgcctgc
    18241 cagctgcgtg gctttgacca catcttccag gccgagtaac gaaaccagtg cggtagattt
    18301 gaggatcacc tgccagttgt tgccaatgcc tggcagggcg taacgcatca tcgccggaaa
    18361 catgatccgc cgaaacactt gcccacgagt aaaaccgaac gccctcgccg cctctatatg
    18421 tcctttcggc actgccataa aagcaccacg gaacgtttcg gtaaaataag caccgtagat
    18481 aaaaccgaga gtgataatac cagcgaccat cggatcgata tcaatctgcc cgacgcccat
    18541 cgcctccgtc accgtgttta gcgcaatctg caaaccgtag aaaatcagca gcatcaacac
    18601 taagtccggc acgccacgaa tcagcgtggt gtacccttcg aaaatcagcc ccgaaagccg
    18661 attttgcgag agcttaccgc cagcgccaat taaaccgatg attacagcga gcactacaga
    18721 gctgatagcc agctccagcg tgacgagcgc accctgtaaa ataacacctg aaaacccata
    18781 caacatgctg cctgtcctgt cgtgtgtggt gaattaccgc ctcttgtctc cctccggtta
    18841 cccgaaggga gcgggctttc tttcaccgat ggcgattaac caccataaac atcaaaatcg
    18901 aagtactttt tcgctaattt ctcgtaagta ccgtcagcgc gcatttcggc aaaggctttg
    18961 ttcagtgcct cgcgcagttc gttatcttct ttacgcaggc ccatgccggt ccctacgcca
    19021 aacagttttt catctttaac agacgggcca ccgaatttgt aatctttacc gacaggttgt
    19081 ttgaggaaac cttcgctggc agcgacctca tcctggaacg cggcatcaat acgtccggca
    19141 gtcaggtcag aataaatgtt gtcctgcccc tgatacgaga cgatttcaat gccttttggt
    19201 gcccaatgtt cattaccgaa cgtctcctgg gtagtgccct gcaatacgcc tacccgtttg
    19261 cctttcagcg actcgactgt cggctgaatg tcagaatttt tcgccaccac cagacgagaa
    19321 tcggcagcgt acagtttgtc ggtgaaggct atttcttgct gacgtttttc cgtaatggaa
    19381 agcgatgaca tgatggcgtc aatcttcttc gcttttaagg acgggattaa cgcatccagc
    19441 ggattttcga caaacgtaca ttgcgtattg atgcgtttgc ataattcctt tgccagatcg
    19501 atatcgaagc caaccagttc gccttgtgaa ttttttgatt caaatggcgc ataggtcggg
    19561 tcggtaccga tgcggatgtt ttgcggaatc gcagcaaatg ccgcagttgc gctggagaag
    19621 gccagaacca gagagagcga tagcaccagt tttttcataa tgtcctcaac tgacagtctt
    19681 tttataggga atatttacag gtttgttaca cttatcgtgc cataaaattg accatcaagg
    19741 caaaatatcc cgccccgtta gacgtattat ttttcattaa aaatgcttaa gtatgcattt
    19801 caatgcacca cgatagtgca acaaacctgg cgtgcaccat gatgatcatt ccatcaggta
    19861 cagcttccct gcgacgtatc agtcaccgta gacattaaag tcaaaatact ttttcgccat
    19921 cttgtcgtag gtgccgtcct gacgcagctc gccaagcgcc ttattgaagg cagccgtcag
    19981 ttcagcatca tctttacgta gccctacacc ggtgccatca ccgaagtatt ttttgtcttt
    20041 tactgatgag ccagcaaagg cgaaatcttt accagcaggt tgcttgagga atccttcgct
    20101 ggcagcaact tcatcctgca gtgcagcatc cagacgtcct gcagccagat cggaatagac
    20161 caaatcctgg ttggcatagg ccaccacatc cacacctttg ctacgccagg tctcgttagc
    20221 gtaagcttcc tgggttgatc cctgcagcac accaacatgt ttacctttca gtgagtccag
    20281 cgttggctga atcggcgaac ctttggcggc aatcagacgg gaatctgcgg cgtacagctt
    20341 gtcggagaag gcaatctcct gctgacgttt atcggtaatg gaaagcgacg aaataatggc
    20401 gtcgattttt ttcgctttca gtgaggggat cagcgcgtca aagtcactgg caacccaggt
    20461 acatttcacc tgcatccgtt tgcacatctc gttaccgaga tcgatatcaa agccgacaaa
    20521 gtcacctttg gcatctttcg acgagaacgg tgcataggtg gtatcggttc cgatacgtac
    20581 cgtctccggc aatgccgcat agctggaagc cgctgcggaa ataccgacta acaaagacag
    20641 agcgagaatc gacttcttca tacataaccc tcaagtgaaa atggcttttt tatgttgtgt
    20701 attgtgttgt gtgtttgcag gcttgttcat gcaggtctta tgccatcttg acgacaacag
    20761 taacattcaa cgttaaatat gttaataaga cgttgcatta ttgtcctaaa gttgaagata
    20821 gcaggtatag cggttgaatc gcagcgtttc gattgaatgg cagaaacaaa atgtcgagga
    20881 tttgatcgcg gttgcaaaat tgccctgaaa cagggcaaca gcggagttat gcgccctgcc
    20941 agcgggcaaa gagatcttca ggaagggtta tcgcaaactg gtcaagaaca cgattaaccg
    21001 tctgatttat cacatcatca agggattgcg ggcgatgata aaacgccgga acgggaggca
    21061 taatcaccgc accgatttct gctgcctgcg tcattaaacg cagatggcct aagtgcaatg
    21121 gtgtttcacg cacgcagagc accagtgggc gacgctcttt cagcaccaca tctgccgcac
    21181 gggtcagtaa gccatcagta tagctatgga caatgccgga cagggttttg attgaacagg
    21241 gtaaaatcac catccccagt gtctggaaag aaccggaaga gatgctggcg gcaatatcgc
    21301 gcgcatcatg cgtgacatca gctaatgcct gcacttcgcg cagggaaaaa tccgtttcga
    21361 gggataaggt ctggcgcgcc gcctggctca ttaccagatg cgtttcgata tctgtgacat
    21421 cgcgcagaac ctgtaataag cgcacaccat aaatcgcgcc gctggcaccg ctgatgccca
    21481 caataagtcg tttcataaat agttgccctg tcagacttgc aggcagactt tgcaggattt
    21541 cggcgggagt tgcaagtcag ggtgccagac cggcaccctg agcgaaggca tcatccttcg
    21601 ttatgcattt cgagattttc cacttcgttc tgacgttgca ctgctttggc gtcgtcatta
    21661 cgtaaagtat cgaggaaatc gaggtagccc tgatcaacat ctttggtgac gtagacgccg
    21721 ttgaacaccg agcattcaaa ctgctggata tccgggtttt cagcgcgaac ggcttcgatc
    21781 agatcgttca gatcctggaa aatcaacccg tcagcaccga tgatctggcg aatttcatct
    21841 acttcgcgac cgtgagcgat cagttccgtg gcgctcggca tatcaatacc ataaacgttc
    21901 gggaagcgaa tttccggtgc cgcagaagcg aggtacactt tcttcgctcc ggcttcgcgt
    21961 gccatctcga taatctgctc agaagtggtg ccacggacga tagagtcgtc gaccagcagg
    22021 acgtttttat cgcggaactc ggcgcggttg gcgttcagtt tacggcgcac ggacttacga
    22081 cgcagctgct gacccggcat gatgaaggtg cggccaacat agcggttttt aacgaagccc
    22141 tggcggtacg gtttgcccag aatacgagca atttccagcg cgatatcaca cgaggtttcc
    22201 gggatcggga tcaccacgtc gatatccaga tcttcccatt cgcgggcaat tttctcgccc
    22261 agcttcgtgc ccatattcac acgcgcgctg taaacggaaa ttttgtcgat gaacgagtcc
    22321 gggcgagcaa agtatacata ctcaaacagg cacggattgc tgaccggatt gtcagcacat
    22381 tgacgggtaa acaactgccc ttcttcagtg atgtaaatcg cttcgccagg cgcgacgtca
    22441 cgcaggaaat caaagcccag tgtatcgagc gcaacgcttt cggaagcgac catatattct
    22501 gtgcggttct cgtcaatatc acgttttccc agaaccagcg gacgaatacc gtttggatcg
    22561 cggaaagcaa ccataccgtg gccgataatc atcgccacac aggcatacgc gccgcggatt
    22621 aagcggtttg tagcagcgat ggcggcgaaa atgttgtcgg cttcaagcgg gtagtggcgg
    22681 aagttgtcca gctcgctggc gaagatatta agcagaattt ccgagtcgga agtggtgttg
    22741 atgtggcggc gtttttcttc aaacagtttt ttacgcaact cgtgagcgtt ggtcagattg
    22801 ccgttgtggg caagcgtaat gccatacgga gagttaacgt aaaacggctg cgcttcagag
    22861 gcgctggagc tgccagccgt agggtaacgc acatgaccaa tgcccatatt gccctgcaaa
    22921 cgctgcatat ggcgagcttc aaatacatcg ctcaccagcc cgttcgcttt acgcaaacgg
    22981 aagcagttat tggcatctat ggtgatgatg ccggcggcat cctgaccgcg atgctgaagc
    23041 accgttaagg catcataaat cgactggtta accggcataa caccggcgat accgacaata
    23101 ccgcacatac gtctttttcc tcgttaagcc acatctcaga gcacttacgc tctgggcaag
    23161 aaacttgacg agctttgcag ataatcaaaa aaccatctga tgataaaact gaactgcgga
    23221 atcagttgtg atttactcca gtcttcgctt ttcgacaccc cggtaaagga gtcgagaaag
    23281 aagagaatag cagcaacaat caacacaccg cgcaatgcac cgaaacagac gcccagcacc
    23341 cgatcggtgc ctgacaaccc cgttttctcc accagcaggc ctatcacgaa gttcacgata
    23401 gcgccaacga tcagggtggc gataaacagg accgcgatgg caatcccatt tcgaaccagt
    23461 tcgtcttcaa agcccgtaaa ccagactgac aggtaagtgt agtaatgact ggcaacaaag
    23521 aaagcacaac cccatgtcac cagcgataac gcttcacgaa caaagccgcg gatcaggcta
    23581 accagagagg aaaaagcaat caccgcgatt atggcgtaat caatccagac catatgtgtc
    23641 ccacgatttt acgccctgtc atcctgttcg gggcgcattc taacagaaaa agaaaacgtt
    23701 tgcgtaggga tttccttccc gcgcatcaat aaaaatggcg ctgaaaaaat attcaacgcc
    23761 atcgactttt tatgcctttg cggcatcgga caatgcgtgt cggatgcggc gtaaacgtct
    23821 tatccgacct acggttctac ccctgcgtag gcctgataag acgcaccagc gtcgcatcag
    23881 gcaacggctg tcggatgcgg cgtgaacgcc ttatccgacc tacggttcta cccctgcgta
    23941 ggcctgataa gacgcaccag cgtcgcatca ggcaacgcct gtcggatgcg gcgtgaatgc
    24001 cttatccgac ctacggttct acccctgcgt aggcctgata agacgcgcca gcgtcgcatc
    24061 aggcaacggc tgtcggatgc ggcgtaaacg ccttatccga cctacggttc tacccctgcg
    24121 taggcctgat aagacgcgcc agcgtcgcat caggcaagac cgtattaatt cggcgtatag
    24181 cccattacca cgccacttaa gccagaaagt tgcttcaact cacccagcga acctttcagc
    24241 ttatctttcg aggcatccgg cccaaccaga atacgggtaa ttttaccctg cactggcgtg
    24301 gatggcgacg tataaacccg ataaccggca ccgcgcagct taccgacaat ctcattcact
    24361 ttatcggcat ttttcagcgc gcccagttgc acaacatagg ctttacccgt tggcgctgct
    24421 ttttcttcca cgaccggctt cggttctggc ttcggcgcag gtggtgcttc aaccttcggc
    24481 ttaggcggct ccaccggttt cggcttcggt gggacgaccg gtgcaggttc cggttcaaac
    24541 tcggtgttat tggctgcaat agtggccgga tcgagcgacg gtgctgcggc atcacctgcc
    24601 cgcacctctt ccgctgcgcc ttccggcggt tgcgtcggca acgcctgggt ggcggctggc
    24661 atcatatcag gctcatcgcg atcgcccgct tttggcacca gcgggatagc cgcaaactca
    24721 tcctgataat gttttttctg cccgtccagc agccctggaa gtacaatcac ccccagcgcc
    24781 accagcacga tcgtgcccac taaccgattc tgaaacttac ttgccaccgc ttctcctcgc
    24841 gtcaatcact tccatgacat gtgcgaccgt gtggaacgaa ccacacacca gcacggtgtc
    24901 ttccgcttta gcgtccgcca ttgcggcatc ccatgcctgc gcaacgctat caaatgattt
    24961 gccgttaccc aaatgctcaa gcagttgttc tgccgtggca ccgcgcggcc cttccagtgg
    25021 cgcacaatac cagtcatcaa ccacgctttt caaccaggcc agagttccgg caatatcttt
    25081 atcatgtagc ataccgataa ccgccagcac gcgcccgttt ttcggtagcg ttttcatacg
    25141 cccggtgaga tattccgccg catgtggatt atgcgcgaca tcaaaaataa cgcgtggcga
    25201 ctcgctcaca atctggaaac gtcccggcaa aattgcgctg gcaatcccgt cgcgaatggc
    25261 attttcactg acctccagcc cgctggcacg cagtgccgcc agcgccgttg cggcattcgg
    25321 ttgcgggaca agcggcaacg gcaggttttc cagcgtgccg tgagcatcgc taaacgccca
    25381 gtcatgatcg gtgacggaat agttccactc aacgccccga cgttgtaaca gtgcaccttt
    25441 ttcctgcgcc acatcagcaa tggtagaagg catttccggc tcaccgacaa ttgccggttt
    25501 ttcgctgcga aagatgcctg ctttctcgcg accaatactt tcgcgatctg gccccagcca
    25561 gtcggtatga tccagcgcaa tactggttac taccgcgaca tcggcgtcga caatattggt
    25621 tgcgtccaga cgaccgccca gccctacttc cagaatcacc acgtcaagtt gtgcctgttt
    25681 gaacagccac aacgccgaca gcgtaccgta ctcgaaatag gtcagggaaa tatcaccgcg
    25741 tgccgattca atctccgcaa aagaggcggt gtgggccgat tccggcaatt cctgaccctg
    25801 cacacgtacg cgctcggtat aacacaccag atgaggcgaa ctgtagacgc ccactttgta
    25861 ccctgccgcc atcagaatcg actccagcgt acggcaggtg gtgcctttgc cattcgtacc
    25921 cgcaacggta aacacaaatg gcgctggttt cagaacgcca agacgcgccg cgacctggct
    25981 cacgcgctca aggccgagat cgatagtttt actgtgcagg ttttccagat aagaaagcca
    26041 cgaagccaga ggcgacgcgg cttgaggagt gcgtttgata atcatggtat ccgctgattc
    26101 gttacggtga gaatagcaaa agggcagagc cagtggccct gcccttatca gttatcaggc
    26161 ctcaggttcc tgatccggta ccgggggtac cactacgcct tcacgcggcg cttcaggatt
    26221 cggcgctggc agattcatca acttcgccag aatgctcgcc agtttcaggc gcatttccgg
    26281 acgacggacg atcatgtcga tcgcgccttt ctcgatcagg aattcactgc gctggaatcc
    26341 tggcggcagt ttttcgcgaa cggtctgttc gataacacgc ggaccggcaa agccgattaa
    26401 cgctttcggt tcagcgatgt tgagatcgcc cagcatggcg aaacttgcag aaacaccgcc
    26461 catcgtcggg tcggtcagca cggagatgta cggcaagccg cgctcctgca tttttgccag
    26521 tgccgcagag gttttcgcca tctgcatcag cgacatcagt gcttcctgca tacgtgcgcc
    26581 accagaggcg gagaagcaga tcagcgggca gttatcttcc agcgcctgct caacggcacg
    26641 cacgaaacgt gcacccacaa cagaccccat tgaaccgccc ataaaggcga actcgaatgc
    26701 cgcagcgaca accggcattc catacagagt gcctttcatc accaccagcg catctttttc
    26761 gccggtttct ttctgcgcag atgccagacg gtctttatac ttcttggagt cacgaaactt
    26821 cagcacatct ttcggctcaa gctcgctacc cagctccaca aggcttcctt catctaacag
    26881 gctatgcagg cgattacgcg ctgtcatacg catgtgatgg tcacacttcg gacagacctc
    26941 aagattacgt tccagctcag cgcggtataa aacctgaccg cagctatcac acttagtcca
    27001 caccccttca ggaatgctcg ccttgcgggt gggagtaatg ttgcttttaa ttcgttcaat
    27061 ccagctcatt agggaccttt ctgtctgaac ctggttcgat gccagtttta tctttgggga
    27121 cgcataatgc catttttgcc cccaacagac catgaatgtt gcacattaaa acataacagc
    27181 ccgaaacttt ggataaaaaa gtggtcgaac cgcggagtta ctttttattt tgcggcgcgt
    27241 gccgcagcgc gtttgtgacg gattatttcg atgacgcccg gcaaaataga aaccacaata
    27301 atcccgacga tcagcagctt aaggttatcc tgaaccatcg ggattgtacc gaagaaatag
    27361 cctgcgtagg taaaaagcag tacccacaac agtgcgccga tcacgttata agcagcgaaa
    27421 tgacggtacg acatgtggcc cattcccgca acaaacgggg cgaacgttct gacgatcggc
    27481 acaaaacggg cgagaataat cgttttgccg ccatgtttct cataaaactg atgggtttta
    27541 tcgagataac tgcgacggaa aattttcgaa ttgggattac tgaataactt ttcaccgaac
    27601 aagcgcccga tagtgtaatt gaccgcgtca cccacaatcg cggcgatcag catcagcacc
    27661 accatcatat ggacgttgag atcgttggtt tccagtgatg ccaacgctcc ggcaacaaac
    27721 agcaatgaat cacccggtaa aaacggcgtt accaccagac cggtttcaca gaacaaaatt
    27781 aagaacaaaa tggcataaac ccagacgccg tactccgcga ccagctctgc cagatgcacg
    27841 tcaatgtgca ggataaaatc gatgaggaaa taaatcaggt ccatatttgc ctatgccttg
    27901 tactcgtcat ctttcaggct gtaactacgt tggctacagc ctgaaatgtt ccgagcatta
    27961 ttctcgttag tccgccagaa atagcgggcc cattggcggt tttggaagat cataccggtc
    28021 agggtaatcc accgcgacca gatacagccc ttccgctttt gccgttgctg ccgccagcgt
    28081 tctgtccttt gccgccagca actctgctat ccagctctcc ggctggttgt gggcaccgac
    28141 ttccatcagg ctgccgacaa tattcctgac catatgatgt acaaaggcat tcgctttgat
    28201 atcaaccacc acataaggac cgtgacgcgt gacattaata tgcataacgt tgcgccacgg
    28261 ggttcgggac tggcactgca ccgcacggaa cgaggtgaaa tcattctcgc ccagcaagca
    28321 ctgcgcagcc cgatgcatcc gttcagcgtc cagcggttcg taaaaatggg ttaccccttt
    28381 actcagtacc gccgggcgca gccgatgatt gtagatgatg tagcgataac ggcgagccgt
    28441 ggcgctaaat cgggcatgaa aatcatcagg tacagcttta acccaacgca cggcgatgtc
    28501 accaggtaaa ttcgcattta cgcccaacgt ccacgcagcg tctttgcgct gcgcggtagt
    28561 ttcgaaatgc acaacctgcc cggtaccgtg tacccctgcg tcagtacgcc cggcgcagaa
    28621 gacggtgatg ggttcgttcg ccacctggga gagcgccttt tccagcttct cctgcacact
    28681 gcggacttca ttctgccgtt gccagccgta atacttactg ccgtcgtact caatgcccag
    28741 cgcaatttta taaactggcg gttgttgctg gtcggacatt agtacagata ctcctgcacc
    28801 agtttctcgg cgatttttac tgccatcagc gcgccgccaa agcgaacgtt atcggccacc
    28861 gaccagaact ggacttgctc cggcataccg tagtcattac gcacgcagcc aacagaaaga
    28921 tgcggcgtac ccgaagcatc acctacctga gttgggaatt cgttctcttc agagagcaca
    28981 atatcttccc cttgaacaaa cgcatcacgc gcttcttctg ctgccagtgg acgcagagct
    29041 tcaaagttga ccatctgggc atgaccgtag aataccggtg cctggacgac gcttgccgaa
    29101 atcatcagtc cttcgtcctg caggattttg cgtacttcgt caacgatacg acgttcttca
    29161 cgcacgctac cttcgctatc cggcagtaac ggcagcatgt tgaacgcgag ctgacgcccg
    29221 aagaaatctt cttcgtcaat cggaatgccg ttgagcaatt tcgcactctg ccccgctaac
    29281 gcatcgaccg cttttttgcc ctgggcggag gctgaaatca ggctggtaac actgatacgc
    29341 gacaaaccgc cctgatcgat taacggtttc agtgccgcca gcagctggct ggtcagactg
    29401 tctggtacgg cgatgacatt ccggttgcgg taatcggtca gtacaaacgg gtttacttcc
    29461 ggcaccacca gcggtacgtc gggttcaaga gcaaacaatc cgctgctgtc gatcaccagg
    29521 caacctgagt tggtcgcttc ttcaacccag gctgcggtag cttctttgcc tgcgacaaaa
    29581 aatgccagct gcgcctgcgt ccagtcgaat tcagcagcat cctgcacggt gattgtctta
    29641 ccaccaaagc gcaattgttc gcctgcgctt tcgttacgtg ccagtgcata aatttcccca
    29701 accgggaact gacgttcagc cagcgtttca agcagggctt cgcccacagc gccagttgtg
    29761 cccaggacgg caatgttcca gccttcagac atggtggttt actccagaaa tagcaaagct
    29821 ccctgccaat gttatagcag agagcatgaa gaagagatta acgtgccgga tgatgaacgg
    29881 cgttaaaacc cagtttacac agcaatgatg ccgcactggc gtcatcacaa attacataca
    29941 gagatgacca ttcacggcgc tcaagatagt ttttgcgcag tttatcgaac tcacccggta
    30001 tcccggcgac tttacgcagc ggtgcgtcat cgcggcgcac atcatacacc aaatgcacca
    30061 gccttttcag cgtcggttga tcgagcggtc catgcagagt aatgcgacca aactctggcg
    30121 caggcagtaa tgtatccagc gcaacgtgct gttcatgccc aataaacttg ctataggctt
    30181 caaacacttg cgtagtaccg cgtgctttac cttccagggt atagcctgca atatgcggcg
    30241 tgccgatatc cacttttgtc agcaattcca catttagttc cggttcgcct tcccagacat
    30301 ccagcactac gcttaacttc tggccttcat tcaggcaggt cagcagcgcg gtattatcga
    30361 cgactgcgcc acggcaggcg ttaatcagaa tcgctccggg cttcaggcta cggatcagtt
    30421 tttcatccgc cagatgcagc gttttgtacg gcccgtcttt gaagagtggc gtatggaaag
    30481 tcagaatatc cgcgcgctgg actaactcat ccagcgagcg gaaatcaccc tcatccccac
    30541 agtcggcgcg aggcggatcg caaagtaagg ttttaatccc taacgcttcc agtcgtgcct
    30601 gtaaacgccg accaacgtta ccaacgccta cgatccccac ggtacgctcg tgcagtgaaa
    30661 atccatcgcg ttcggcaagc atcagcaggg aggagaaaac atattccacc accgcaatcg
    30721 cattacagcc aggtgcagcg gaaaaaccaa ttcccgcctg cttcaaccat gcttcatcga
    30781 catggtcggt ccccgcagtg gcagtgccaa caaatttaat gggttttcct gccagcaaag
    30841 attcattcac tttcgtgacc gaacgcacca tcagcgcatc cgcgtctgcc agttgagcga
    30901 cggggattgg acgcccggga accgcgatca cctcacccaa acggctaaat aagtcgcggg
    30961 cataaggcat attttcatca acaaggattt tcacgtttgt gttacctgta tgagacgaga
    31021 gttaaccgga caagtgtgcc ataatctcgc ggccaggcat acttgcgaag atttcaggta
    31081 taaggatacg taatgataca acctatttcc ggccctcctc ctgggcaacc accaggtcag
    31141 ggagataacc tgccgtctgg cgcgggcaat cagcctttat ccagtcagca acgtacttcg
    31201 ctggaaagct taatgacgaa agtgacctca ctgacgcaac agcaaagagc agaactgtgg
    31261 gcgggtatca ggcacgatat tggtctgtcg ggagattcac cgctgctttc gcgtcactgc
    31321 cctgccgctg agcataatct ggcgcaacgt ctgctggccg cgcaaaaaag ccattctgcc
    31381 cgccagcttt tagcgcaatt aggggagtat ttacgtctgg ggaataatcg tcaggcggtc
    31441 acggattata tccgtcataa ctttggtcag acgccgctga atcagctctc accggagcaa
    31501 ttaaaaacca ttctcaccct gttgcaggaa gggaagatgg ttattccgca accgcagcag
    31561 cgcgaggcga ccgatcgtcc tctattaccg gcggagcaca atgcgctcaa acagctggtg
    31621 accaaacttg cggcggcaac gggggagccc agcaaacaga tctggcaatc gatgctggaa
    31681 ctttccgggg tgaaagatgg cgagttaatt ccagcgaaac tgtttaacca tctggtgacc
    31741 tggctgcagg cgcgtcagac gctaagccag caaaatacgc cgacgctgga atcactacaa
    31801 atgacgctaa aacaaccttt agatgccagt gaactggcgg cgttatcggc atatatccag
    31861 caaaaatatg gcctttctgc gcaatcatcg ctttcttctg cccaggccga ggatattctt
    31921 aatcagcttt atcaacggcg ggttaaaggg attgatccgc gtgatatgca accgctgctt
    31981 aatccttttc caccgatgat ggacacgttg caaaatatgg caacgcgtcc cgcgctgtgg
    32041 atactgttag tcgcgattat cctgatgctg gtctggctgg ttcgttaacc ccgacgaaac
    32101 gacagtatcg tgacaataat tcccagcacc gcagagatcg ccccggcaag aaataccgaa
    32161 gagtaaccaa acgtggtcgc cagcattccc gcaagcggcc cggagacgcc gagggcgata
    32221 tcctgaaacg cggcgtaacc gcccagtgcg gtgccgcgaa cttgtgaggg gacgcgttta
    32281 accacctcca cgcccagcgc aggaaagata agcgaacatc cggctccggt taacgccgcg
    32341 cccgctaatg cgacccacgc ccctggggct tgccagagca gcaacaagcc caccgtttct
    32401 acaagcagag agacaatcgc cactttcacg ccgccaaaac ggtccggcat ccagccaaac
    32461 atgacgcgca tcacgacaaa tgcgccgcca aacgcggtaa gagtaaagcc cgccatcgcc
    32521 catcctttgc tggcaaagta gagcgaaacg aaagtcccga taaccgcaaa accaacgcct
    32581 tgtagtgcca gacctaaccc tggtttccag ataagtccga caacgctcca cagagatgga
    32641 cgttctcccg ccagggctgg tactttgcgc actgtgccgt tacaggccca cgccagtaag
    32701 ggtaatgcca ttgtggtgag cgccagtgcg gcaaaaccgt aatggctatg aatcaacagg
    32761 ccaagcggag caccaacagc gagggcaccg taaatcgcca ttccattcca tgacatcact
    32821 ttgccagagt gttttggccc tacgatgcct aacccccagg tcagagcgcc tgtcagtaac
    32881 tggctttcac caaacccaag aatcaaacgc ccgatgacca acagggcaaa tttgaacggt
    32941 gcggagacag gcaaaatcgc cgccagcagc aacgcgccgc cagccagacc acaagctaac
    33001 atcccctgaa gcgccgaacg ttttgcgcca tattgatcgg ccagtcgccc ggcatagcca
    33061 cgcgtcagca ccgtagccag aaactgaatg ccgacggcaa tgccgaccat ggtattgcca
    33121 tagcccagat catgatgaac aaacaacggg ataaccggca acggcaaccc tacggtcatg
    33181 taggtgagaa aaaccgcaaa agcgatgcgg aagagcgaaa aattggcaga agatcgtgtt
    33241 tcggtttggc ttacagcagt catgcattac tccagaatgc agcgcaaggc gaggagtatc
    33301 cccgtctcat ctctctggtt tcagggttac agtgcgttgg caggatttaa cgcgtacgtc
    33361 ttttcagaag gaaatcgaca aagcggggag tttgcctgga actggcggcg attgtcaatg
    33421 atgtgaaaaa gggaaccatc aggttccctt ttgcgttagt gccggaggcc gcattggcgc
    33481 gtaacgtcgg atgcgacgct ggcgcgtctt atccgaccta ctccggccta ctgcgaatta
    33541 atctttcagc ttgcgcatta ccagcgtggc gttggtgccg ccgaagccga agctgttaga
    33601 cataacagtg gtcagttcgc gatcggtcgt ttcggtcacg atgttcagac ccgcagcctg
    33661 ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaag ccgtgttcca gcatcagcag
    33721 agagtagata gcttcctgta cgccagcagc gcccagagag tgaccggtca tggctttggt
    33781 tgcagaaatc gccgggctct tatcgccgaa cacttcacgg atagctgcca gctctttcac
    33841 gtcgccaacc ggagtcgaag tgccgtggga gttcaggtaa tcgattgggg tatcaacgcc
    33901 atgcatcgcc atctgcatac aacgtactgc gccttcgcca gagggagcaa ccatgtctgc
    33961 gccatcagac gttgcgccgt agccaacgat ttcagcatag atgtgagcac cacgcgccag
    34021 cgcgtgttcc agctcttcaa ccaccaccat accgccgccg ccagcgataa cgaaaccgtc
    34081 acggtgagca tcgtaagtac gggaggcttt ttccggggtg tcgttgtatt tagtagacag
    34141 cgcacccatt gcgtcgaatt cgcaagccat ttcccagcac agctcttcgc cgccgccagc
    34201 aaacacgatg tcctgtttgc ccagttggat ctgctctact gcgttaccga tacagtgtgc
    34261 ggaagtcgca cacgcggagc tgatggagta gttaacgcca tgaattttaa acggcgtggc
    34321 gaggcaggca gaaacgccgg atgccatcgc tttggtgacc acatacgggc caaccgcttt
    34381 caggccgcgc gggccgcgca ttgcgtcagc gccgaacacc tggaaacgcg gggaaccgcc
    34441 gccagaacct gcaatcaggc caacacgcgg attattctgg taagcttccg gagagaggcc
    34501 cgcatctgcg attgcctgct ccatagaaag gaatgcataa atggatgcgt cgctcataaa
    34561 gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt tttacgttgc cccagacgtg
    34621 gctacgcatg ccggaatcct tcagctcctg agagaaagtg atccctgaac gtccttcacg
    34681 cagagatgcc aggacttcct gctggttatt accgatgctg gaaacaatgc ccaggccagt
    34741 aatcactgca cgtttcattc aatacctctg taagtcgcac atagagtaag tttcgaatgc
    34801 acaatagcgt acacttgtac gccgaacaag tccgatcagc caattaatag agaaatttgc
    34861 gcagccttac acacatcgct aagatcgagc taccgcctgt aagacgagta acttacgtga
    34921 aacactactc catacaacct gccaacctcg aatttaatgc tgagggtaca cctgtttccc
    34981 gagattttga cgatgtctat ttttccaacg ataacgggct ggaagagacg cgttatgttt
    35041 ttctgggagg taaccaatta gaggcacgct ttcctgaaca tccacatcct ctgtttgtgg
    35101 tagcagagag cggcttcggc accggattaa acttcctgac gctatggcag gcatttgatc
    35161 agtttcgcga agcgcatccg caagcgcaat tacaacgctt acatttcatt agttttgaga
    35221 aatttcccct cacccgtgcg gatttagcct tagcgcatca acactggccg gaactggctc
    35281 cgtgggcaga acaacttcag gcgcagtggc caatgccctt gcccggttgc catcgtttat
    35341 tacttgatga aggccgcgtg acgctggatt tatggtttgg cgatattaat gaactgatca
    35401 gccaactgga cgattctcta aatcaaaaag tagatgcctg gtttctggac ggctttgcgc
    35461 cagcgaaaaa cccggatatg tggacgcaaa atctgtttaa cgccatggca aggttggcgc
    35521 gtccaggcgg cacgctggcg acatttacgt ctgccggttt tgtccgccgc ggtttgcagg
    35581 aagccggatt cacgatgcaa aaacgtaagg gctttggacg caaacgggaa atgctttgtg
    35641 gggtgatgga acagacatta ccactcccct gctccacgcc gtggtttaat cgtacgggca
    35701 gcagcaaacg ggaagtagcg attatcggcg gtggtattgc cagcgcattg ttgtcgctgg
    35761 cgctattgcg tcgcggctgg caagtaacgc tttattgcgc ggatgaagcc cccgcgctgg
    35821 gtgcttccgg caatcgccag ggggcgttgt atccgttatt aagcaaacac gatgaggcgc
    35881 taaaccgctt tttctctaat gggtttactt ttgctcgtcg actttatgat tcattacccg
    35941 ttaaatttga tcatgactgg tgcggcgtca cgcagttagg ctgggatgag aaaagccagc
    36001 ataaaatcgc acagatgttg tcaatggatt tacccgaaga actggctgta gccgttgagg
    36061 caaatgcggt tgaacaaatt acgggcgtta cgacaaattg cagcggtatt acttatccgc
    36121 aaggtggctg gctgtgtccg gcagaactga cccgtaatgt gctggaactg gcgcaacagc
    36181 agggtttgca gatttattat caatatcagt tacaggattt atcccgtaag gatgactgtt
    36241 ggttgttgac gtttgcagga gatcagcaag cgacacacag tgtagtggta ctggcgaacg
    36301 ggcatcaaat cagccgtttc agccaaacat cgtctctgcc agtgtattcg gttgccggtc
    36361 aggtcagcca tattccgacg acacccgaac tggcaaaact gaaacaggtg ctgtgctatg
    36421 acggttatct cacgccgcaa aatccggcga atcagcacca ttgtattggt gccagttatc
    36481 atcgcggcag cgaagagacg gcgtacagtg aagacaatca gcagcagaat cgccagcgat
    36541 tgattgattg tttcccacac gcacaatggg cgaagacggt tgatgtcagt aaaaaagagg
    36601 cgcgctgcgg tgtgcgttgt gccacccgcg atcatctgcc aatggtaggt aatgttcccg
    36661 attatgaggc aacactcgtg gaatatgcgt cgctggcgga gcagaaagat aaggcggtaa
    36721 gcgcgccggt ttttgacgat ctctttatgt ttgctgcttt aggttcgcgc ggtttgtgtt
    36781 ctgccccgct gtgtgccgag attctggcgg cgcagatgag cgacgaaccg attccgatgg
    36841 atgccagtac gctggcggcg ttaaatccga atcggttatg ggtgcggaaa ttgttgaaag
    36901 gtaaagcggt taaggccggg taatctgctc tcgcagtgtt tgccggatgc ggcgtgaacg
    36961 ccttatccga cctacgtgtg acgtgtaggc ctgataagac gcgataagcg tcgcatcagg
    37021 catggtgctc cagacgccgc aacttactgc tgtgacgcct gctgaaacag gttttcccac
    37081 atatcggtca ccagcgcctg gtcacgcggc gacaattcac cagcaccaat ggctttttcc
    37141 agactctggc taacggtcgt atgcaccgcc tgagcggagt ggtcatcacc actttccagt
    37201 tctgcgatgg ctaatgtaag gtggccacgc aaatacccac tggcaaacaa ctcatcatca
    37261 ctggcatggt caaccatacc gtcgattaat gccagaatgc gtgattcaaa ctccgcgatc
    37321 atcttctttc ctcattgtaa aacgtggcgc tggcttcagt tgagcgcttc cggccacggg
    37381 aactgttccg ccgtaagttc cggcgtgtga taataatttt gtagtgcttt aatgaaacgt
    37441 gccgggcgtt ccggaatgcc atttgttaga taatccatca cctgcgcatg tactcgccgt
    37501 tgaaacacca cgcggtccgg ttcgaagtcg ccttccagat tgtcgcaact gacgttaaac
    37561 gggtatcccg ccgccacgca gaacaaccag tcgagcgcct gcggcttcac ttcaacatct
    37621 tcaaactggc tttgcgtttg ggcatcgcga ccgtccgggc aataccagta gccgaaatca
    37681 acctgctcgc ggcgcgcttt cccggcaata caccagtgcg aaatctcatg aattgcgctg
    37741 gcataaaagc cgtgggcaaa gacgattcgg ttatacggta cttccgcatc agcgggaaga
    37801 tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa aatcatcggc aaagcagcta
    37861 ttaaaaattt caatcaactg ctcatagtgg tgtgtactgt tcattagttc atccccaacc
    37921 agtggaggat ctcctgtccg tggctatcat aaagtaattt ggcactcatc accgccgaga
    37981 cgataacgat catcgggcgg atcagttttt gtcctttgct caacaccagt cttgaaccca
    38041 tgcgcgcgcc aaggaactgc cccaccagca tcacaaagcc cgtcgcccaa atcactttgc
    38101 cgccgagaat aaacagcagt aaaccgccga tatttgacgt tgcgttgagt aatttggcgt
    38161 gagccgtggc tttggcgagg ttgaatccgc acagcgtaac aaaggccagc gcgtaaaacg
    38221 atccggctgc cggaccaaag aatccgtcat aaaaaccgac gcagccaccg gcaattaacg
    38281 cgaagggcaa accgtacatc cggcgctggc gatcttcttc acccagcttt ggcatcagca
    38341 agaaatagag gccgatacaa atcaccagaa tgggcaaaat ctgccgtaag acatcagcct
    38401 gaacgtactg taccaacagt gcgccgctca ttgagccgac aaaggtcatg gcaatattaa
    38461 gtttctgatc gcttaaacta accactttgc ggcgaataaa gtagatagtg gcggaaatag
    38521 agccgccgca ggcttgcagt ttattggttg ccagagcatt agcgggagac atccccgctg
    38581 ccatcaatgc cggaatggtg agtaacccac cgccaccggc aatcgagtcg ataaatcccg
    38641 ccagcatggc gacaaaaaag agaactccca gcaacagcgg ggaaaccata aacaggctat
    38701 taaacgtttc cattagatca cgtgctcatc cagtagcgcc tggcaggaag gcggcaacgg
    38761 aggcggtgtc ttcttctcag gctttgttgt tcccggtttt ggaggttcaa accagctttg
    38821 cagttctgcc ccgcaaccat cgcctggtgg cggtaaaggt tgatcttcac actccagact
    38881 atcggcagga caacgtaatc gtacatgcat atgcgcacga tgctggaacc agggtcgcac
    38941 tttgcgcaac cagtcgcgat cggtgcccgc atcaaggcaa agttgttgtt taatcgccgg
    39001 attaacaaaa atgcgcgtga cgtctttgtc ctgggcggcg agtttgatca agctgaaaat
    39061 ttctggcttc cacagcgtgg agacaacgtg tttaccgtcg cgggaaacta agtccagtgc
    39121 ttgcgggcgc aagagctgcg cggaggtccg gcgtgttttc ggcagttgca gaaagatatc
    39181 gacatccagt ccggtctggt ggctggcgtg accgccgttg aaacgcccac cagcaggcat
    39241 tcccatatcg ccaatcaaca ccgtacccat gcccagattg ctcacctgac tactcaggcg
    39301 ctggataaac atcaccaggt ccgggtgacc gaaatagcga cgctgatcgg tacgcatgac
    39361 ctgataatgt tcggactgta tcggcaacgt gtcagcgccg acgatacaac cattggaaaa
    39421 actgcctatc gattgtgcgc tacccggcac aggttgggtt attttttgcc acggcgtcgc
    39481 tgccaggctg acgctactgg caagcagggc cagcagcgca atcgcggttt tgttcatttt
    39541 ttaccagcgt ggaatatcag tcttcacatc ggcattttgc gcccgctgcc gtaacaggtg
    39601 atccattaaa acgatcgcca gcatcgcttc tgctatcggc accgcgcgga tcccgacaca
    39661 gggatcgtga cggcctttgg tgatcatctc aacttcttcg ccaaagcggt taatggtgcg
    39721 ccccggcacg gtaatactgg aggtcggttt cagtgccata tgagcaatga tttgctgccc
    39781 gctgctaatt ccgccgagaa tgccgcccgc atggttactc tggaaaccgt ctttggtgat
    39841 ttcgtcgcgg ttctggctgc cacgcagcgc caccacgtca aaaccatcac caatttccac
    39901 gcctttcacc gcgttaatgc tcattagcgc atgggcgatg tcggcatcca ggcgatcaaa
    39961 gaccggctca ccaagcccgg caggaacgcc actggcaaca acggtgactt tcgcgccaat
    40021 agagtcgccc tcttttttca gcgcgcgcat cagttcatct aacgcgtcga ttttgtccgg
    40081 gtccgggcag aagaatggat tttgctcgac ctgcgaccag tctttgattt ccagcggaat
    40141 gtcgcccatc tgggtcaggc agccgcgaat ttcgatgcca aatttctcag cgagatattt
    40201 tttggcaatc gcccctgctg caacgcgcat ggcggtttca cgggcggaag aacgtccacc
    40261 gccgcgataa tcgcgcagac cgtatttttg ttcgtaggtg taatcggcat ggccaggacg
    40321 gaaaacgtcc ttaatcgcac tataatcctg agaacgctga tcggtatttt cgatcaacaa
    40381 accaatgctg gtgccggtag taacaccttc aaaaacaccg gagagaattt tgacctgatc
    40441 tggctcacga cgctgggtgg tatagcgcga tgtcccaggg cgacgacggt cgaggtcatg
    40501 ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca ccatcgacga tgcagccgag
    40561 cgccagcccg tgcgattcgc cgaaggtggt tacgcgaaag agttgtccaa ttgtgtttcc
    40621 agccatcacg gctccgttat tgttgtgttt gcgtgtttac ttaatcttta taaatcgcga
    40681 aatgttcacg ggccgccagc agctgcgctt tggtgagcat aaacacacca tcgccgccgt
    40741 tatcaaactc cagccaggtg aacggaacat cgggatattg ttccataaga tgtaccatgc
    40801 tgttgccgac ttcacaaatc agtacgccat catcagcaag ataatctgcc gcgtttccga
    40861 gaatgcgacg cgtcagtttc aggccgtcgg tgccagatgc caggcccagt tccggctcgt
    40921 ggcggtattc atttggcagg tcggacatat cttccgcatc gacatacggc gggttagtga
    40981 caatcaggtc gtactgcact ttcggcaaat cacggaacag atcggaacga atcggtatga
    41041 cgttgtggat cagaccgtgt tcttcgatgt tctgttcggc aaccgccagc gcgtcgggag
    41101 agatatctac cgcatcgact tccgcttccg ggaaggcata agcacaggca atggcgatgc
    41161 agccgctacc ggtacacata tctaaaatat gctgcggttg cttgctgata agtccggcaa
    41221 atttattgtt gatcaattca ccaatcggcg agcgcggcac cagcacgcgt tcatcgacgt
    41281 aaaattcatg gccgcagaac cacgctttgt tggtcaggta agctaccgga atgcgttcgt
    41341 tgacgcggcg gatcacgcgc tcaacaatac gatgtttttc gctggaggtc agacgcgcgg
    41401 tgcgcatatc ttccggaata tccagcggca ggtagagcga cggcaatacc agctgtacgg
    41461 cttcatccca cgggttatcg gtgccgtgac cgtaccagat atttgccgcg ctgaagcggc
    41521 tcaccgacca gcgcaacatg tcctgaatgg tttgcagctc atttactgct tcatcaacga
    41581 aaattttatc cacgtattcc tccagggcat gatcgcaata atttcggcgg ctagtttgcc
    41641 atgaagatga cgataaatca gcattcacgc gcggtgagtg aggaaaaata cgtttaaaac
    41701 gatcgattgc gctacgagtc gggtaaactg taggaaaatt agaaatagag acagataaat
    41761 gaaaaagaaa acaacactta gcgaggagga ccaggctctg tttcgccagt tgatggcggg
    41821 gactcgcaag attaagcagg acaccattgt ccatcggcca cagcgtaaaa aagtcagcga
    41881 agtgcccgtc aaacggttga ttcaggagca ggttgacgcc agtcactatt tctctgacga
    41941 atttcaaccg ttattaaatg ctgatggccc ggtgaagtat gtacgccctg gtgtagacca
    42001 ttttgaggcg aaaaagctgc gccgcggcga ttattcgccg gaattgtttt tggatttaca
    42061 cggtctgacg cagctgcagg ccaagcagga actgggcgcg ttgatcgccg cctgtcgccg
    42121 cgaacatgtg ttttgcgcct gcgtgatgca tggtcacggg aaacatatat tgaagcaaca
    42181 aacaccgctg tggctggcac aacatccgca tgtcatggcg tttcaccagg caccaaaaga
    42241 gtatggtggt gatgccgcat tgctggtgtt gattgaagtg gaagagtggc taccgccgga
    42301 gttgccatga atattagggg gcggatgcgt tttgccattt cgccctgaat gctggtatcg
    42361 aatgccggat gcggcgtaaa cgcctgatcc ggcttatcaa aatgtgcaaa ttcaatacgt
    42421 tgtgattcag ttgtaggcat gataagacgc gtcagcgtcg catcaggcat caacgcacaa
    42481 ctacagttca ttcagtacat actggaaacg gcatgtagaa tcaattggct cacggctact
    42541 ggtattcacc tgtaccacaa tgtcgtgtag ctttctatcc gtcaatggtt gacgtgaggg
    42601 ggtgactgtc acctttgcat tatcacaacc atacgaggta aaggttaata agtcagaaac
    42661 agcacgacaa tatgcccctc tggaatcaaa aatactgcca tcgcagagtt tgccgctttc
    42721 catcaggtag gtcgaaagcg tagtcgtgat tgtcgaacca tcggattgca atcgcagcgc
    42781 tttattatgg ctaacataat tatcactcca ggatatatca aacgaagcga cgggaattgt
    42841 cgggcaaata gaatcatgaa ctgttgggct tgtatacgta ccaaatccaa atgcctcaga
    42901 tactgaataa aatgaggcaa tccacggaca accataatta tcagattcta cagtgacgtt
    42961 aaaagtcata tatgcacaga caaagacacg agtattgata ccagggcaat ttgaggtgtc
    43021 cgtacgtcta taatcagtca aaaccagtgt cggtgccggg ccagatccgc tccaggatat
    43081 taacttgagg gtgtcgcctt ttcttgcatt ggagttgtat tgtccgtagc ttacttctcc
    43141 cggcgtcact accgtgacat agaaatataa actgccggaa gcagtaacgt ttttcatcgc
    43201 ataataaatt ttagtactgg aaaagcttgc actcatgagt ttatcaccgg catagcactt
    43261 tggtgtttct aaactgagag caagtaatat cagacttgtc aaaaatatcg tccatttttt
    43321 cattttaaaa atactcaaaa gttaccgtca ttgtggcggt gaaatcaccc gatgtaacat
    43381 ttcgtccatt tacggtctgt aaccaggcat taaaattgac actgttattt ccctgattta
    43441 atggaaatga ggcacctgtg gtactgttaa taggtacagc cgcccctccg gcagtttcaa
    43501 tgccaatccc aacacccgtt gcagaagatg acgaatcaat cgacagaaat cctggtaagt
    43561 cggtatcttc tgttcccatc aaggtcacct tgacattaaa cgccgtggtg cttttgcaat
    43621 ctttcaactg aaaaactaac ggtacgcgat ctgactgacc acgttgcatt aaatcgctgg
    43681 cagcaattgt ggggaaatga atttctgcaa gtaagttgcc attgattaca ggagtacagg
    43741 atttaccaag caaattaccg gtaaaatgca ggttatcggc agctggggcc gttccgctga
    43801 acaatatcag accactgata agcgcccacg cacggttatt cttcattggt aatccacctg
    43861 taacgtagcc catgcttcaa agtctgactc gggtaagtcg acaccgctct tttttacggg
    43921 tactgccgtt agtaccggaa gggatgtttc attcaccact aagggcgtat gcagcttaaa
    43981 atcgctcccg gcttgtttga ggtgtattcc taatccattg agatccgtgg ctacggccga
    44041 atcgtcaaag tcactctgcg taccactcca ggtcaacgtc atttccattt gagggtcacg
    44101 gacagcggta tcgcaggtaa tggtatacgg cacgacctgt tcgtaattgc taccgttaat
    44161 gtcatcaata ttcacgtccc gaaacgtcaa ctctatattt tgcccattac taatagagca
    44221 attgggtggc tgcactaaat aaccatgaaa agagacattc gagagcgcca taaagctaaa
    44281 gctccacaac attaagagag agatccgttt cattgataat ccaccacaat tgtggcactg
    44341 gcgctaaatt ccgccgccgt tagcgataca ccgctctgca cgacaggaac cgcttccaga
    44401 gccagtgcgc cagatgaaaa gttaaacggt aaccatgcgc caggcgttaa atccaaaatg
    44461 aagtgatcgc ttactttctg aatacggata ccaaaaccgg caatacccgt atcgattacc
    44521 gtttctccgt taattacggt tgtggttgcc tgcatattta agcgcaggtc gtccattgcc
    44581 agattgttgc acacaagcga gtagtcaatg gggcgtttgt aacttacccc atcgatcttg
    44641 gaaatgaaga cattaccaaa ctccacgctc gcacccgtta ccgtacaggg cggcggggca
    44701 tcaacgagca cacgcaagac catcgcaacc gatttactcc ccgccattac aggcgtgctc
    44761 acaatgctac acagcaataa ccacgcccag gccgaataag ctcctttcat gctattgctc
    44821 ctatccctgg cctttgctgt tttgctcttc gtccacctga caggtatttc ccgtacagcg
    44881 gaagaacagc ggcaaacgcg caccataatc attcacatag gtcagcaccg ggacgctatc
    44941 cattttgact tttaacggca cagaggtttt tggcgatatc accatggggg aaaatcccgc
    45001 tgccggattg ccatttttct gcgcactggc gttactgata accacgaagt acggcgtcgg
    45061 gttattgacg gtaaactcct gaccactgag ggttaaggtc accttatgct gccacgggtt
    45121 tttcatgctg acgttttcca gcgcctttgg tcgccagaac aacttaatgc gcgtctgcag
    45181 cgctatctgt agcgtgttag gtttgttcga tttcggcggg atctcacgca cgttgaaata
    45241 gaacacgctc tcgcggtcgg caggcagctt gttgatatcc ggcatgccct gtaccttcac
    45301 ctgaccgttc atcatggaat cgatgcgctg cactggcggc aggaccgtca gtggcgagct
    45361 aatcctgtta ccctgtccat cttccatcca gctttgtgcc agatacggca ttttcgggtc
    45421 gttattgcgc agcgtgacgc tgattgattt atcactttcg ttaaaaatca ggcgtgtacg
    45481 gtccggcgtg acagaggcct gtactgacag gctggcggca gttatcagta acgccagcgt
    45541 cgttgcggcc gttctggcag agcaaaatag attcgtcatg gatcattcct taatcacgtt
    45601 attaattggc tgataccgat actggcgctt ccgttggcat cacctgctgg gtctgctcct
    45661 ggatcaccgg cttaatttca tgaggcatcg aaggagatat cgcccctgtt tgctggcatg
    45721 gtaataacag gccattgaac agatcggctg gcagcgggtc aggcagatga atatcgcagt
    45781 gggccacacc gccccatgaa acgatcatat gctcgccagg ttttacgccc gcgaggtaga
    45841 cgttgccgtc atcgtcaacc agaccgacgt tctgcgcgct gtcgtttttc acttccgcgc
    45901 caaacggcgg ataactgcca tcttgcagac gcagcaccgc catcgctttt tgcccgctga
    45961 tcaccgagaa cttacggtag ccaatggccc cttcggtaag cgtggcctgc accacggact
    46021 gggtggcttc ggcgttttcc ggcaggttgt ttaggtcgat atacgcctgg ttgcggtagt
    46081 agttgttgac gtctgccacc actgccttac cgaacatatt ggtgtaaacc gccgcgccat
    46141 ttccttccac cggaacacca gccacaccgt cggcatcaat cagcagacgc gtaccgccca
    46201 tattctgggt acggtggagc gcgccacctt gtgcggtgag cgtcgcgccg ccctgtaagg
    46261 aaatacccgc cgaggtgtac tgcccttcat ggtagttagc gctgagatcg acctgcgcca
    46321 gcgatccgtc gtggctgtaa taaccgtcaa cgctggagtg attgtcgctg gtgcctacgt
    46381 tcaactggta atgggttgcg tcatcgacac ggctgaaata ccccacctgg ctgctgtccg
    46441 aaccgctgcc gtagttgccg ttatagctga tggtgctgct gtcaccccac ggcatactga
    46501 gcgatatgta cacaccttta tcggcctggt tgtcatactc gtagcggtat ccggtcatgg
    46561 aaatgctcat gttgcggata ctgcccaggt tgaagtagtg cgagagcatg acgttgtagt
    46621 tggtctgttc gtcgcgatcc cagtaggtat ggcgggtgta gttgagataa acagacacac
    46681 cggcatccct gaagttctgg ttataggtgg cggtgtacat ctctttgtcg ttgccggtgc
    46741 gcaccatttc gctgtcgctg gcatcgagat actcgctcat ggtcatgaag ttctcttccg
    46801 agaagcggta tccggcaaaa gtgacgcggc tgttcagttc atcgaagtct ttggaatagc
    46861 tcaggcgaaa cgagttgccg tccagtgaac ctttcccgta ggcggtctct ttatccagac
    46921 gggtatgcga gtgggtgata tcaaaggcca ccgcaccaaa cacagacagg tcgcgaccga
    46981 cgccaagcgc cgccgactga tagtgttcat ctgccagcgc cccgccgtat agcgaccagc
    47041 cgttggcaat cccccaggaa gcttcgccgc cggagaagaa accgccttcc acgtggtgcc
    47101 cccactcctg cgggcggccc atcatcagtt tatagcgcac ctggccgggg cgagtcagga
    47161 acggcatcga ggcggtgttg atgtcatatt cctgcacctg accgttctgt tcttcaatgc
    47221 ggatatgcag cgtaccggag accgaatcgc caagatcctg gatgcggaac ggccccgccg
    47281 ggacctgggt ttcgtagatg acgcggccca actggctgac ggtcactttc gcggtggtgt
    47341 gcgccacgcc ggagatatcc ggcgcatagc cgcgcagatt cggcggcaac atttgatcgt
    47401 cggtgctgat actgccaccc acgtagttaa agccgtcgaa aatatcagaa ttcaggtagt
    47461 cttcgccaag gccaagtttg gcttttagcg acggtaaggc tcgccaggcg tagtagcggc
    47521 tccactccca gtttttttgc gtgtcatcac cgttgataac atcgtcatca ttgcttttac
    47581 tatgcaaata atcagtctgc cagtcggcac gaagacgcca tgcgccgagg ttcaccccaa
    47641 ccgtcccgtt accgctaatt tcattgctgt catccccgcc attttcttca tgtcgtgtct
    47701 gggcggtaat actgtagtca gcaattaaac cagatatacc gtcatcccag cgtgaaggcg
    47761 gatcccagtt gatgtcggta tattcaaggt aagcctgggg taatgaaatg actaacgctg
    47821 actgactcag gtcagcttta atatcaatgc cttccagttg accgggtttc aggcatttgc
    47881 cgtcgtggat ccattgcagt ttttttgcca catcctcttt taagccaaac tgcgcgacca
    47941 gttcaggcgt caggcaggca taggttttac tggcatcgtt ctcagaggcg taccagtaaa
    48001 tatcgtattc ttccgtcagc ggctgtttat ttagttgaac ctgtaaattg tatttcccag
    48061 gttcgacata accctggctg gaaaatcgct tcagatcgat ttttgtgtcg ccttttaact
    48121 ccagaaaacg ggaatcaaac tgaatttcat tttcagccca ggcattgaca taactcccgg
    48181 acattgccaa tgcaacgcag cagggaagta cccgcagtcg gaaaagagaa tggtcaggca
    48241 tacacgtatc catccgtaat tattataatt tgcgcgaatt ccatttaaaa accccatagc
    48301 ccgaaagcta tgggggtatc acttagagat aagtaatctg gaaggtcgtg ttggcttcaa
    48361 aattacctaa atctggcgca tcagcagcgc ccaccagcca ggctttaaag tccagcgtct
    48421 gcttcgcttt gcctttgttg gtcgccgtat cgtttacgat tttctgttcg atacccgcgc
    48481 cgcttttata agaggttccc tgagcgtcac caatggccag gctgacattg ttaaatgccg
    48541 caccagtatc ggtattgtaa atggtgtagt aattgccgct gttggtagaa gacgcgttac
    48601 cggtgaaggt agtggtcatc gttgtctggg tgtcaaacac acagtcttgc agatggatct
    48661 ggaaagcttt cggtgtagaa gtcccgttat ttttcaggat ccctgaacca atgtcaccca
    48721 tatcaacttc cagtttgtga tcatcaggaa cgatagagca cggggaatct tcaatggtgc
    48781 cgtagaaacg tgctgtacca ttgttgccag cggcgattgt tgaagtagaa gcgaacgcac
    48841 ccattaccat tgttgcagca atagctgttt ttacaaactt actcattatt tgtccttaaa
    48901 ccataaataa t
//
LOCUS       DAZFBR010000022        48332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFBR010000022 DAZFBR010000000
VERSION     DAZFBR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.022"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..173)
                     /locus_tag="ACHJZ2_001446"
     CDS             complement(<1..173)
                     /locus_tag="ACHJZ2_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4567592.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYL"
     gene            complement(325..522)
                     /locus_tag="ACHJZ2_001447"
     CDS             complement(325..522)
                     /locus_tag="ACHJZ2_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4567593.1"
                     /translation="MLKQQDMTETARVVFNELSATAPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(738..1118)
                     /locus_tag="ACHJZ2_001448"
     CDS             complement(738..1118)
                     /locus_tag="ACHJZ2_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4567594.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTTKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(1137..2126)
                     /locus_tag="ACHJZ2_001449"
     CDS             complement(1137..2126)
                     /locus_tag="ACHJZ2_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4567595.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(2178..2435)
                     /locus_tag="ACHJZ2_001450"
     CDS             complement(2178..2435)
                     /locus_tag="ACHJZ2_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567596.1"
                     /translation="MNNRTTTVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            complement(2432..3832)
                     /locus_tag="ACHJZ2_001451"
     CDS             complement(2432..3832)
                     /locus_tag="ACHJZ2_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4567597.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLSADVFS
                     ARQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            complement(3829..4707)
                     /locus_tag="ACHJZ2_001452"
     CDS             complement(3829..4707)
                     /locus_tag="ACHJZ2_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4567598.1"
                     /translation="MMTFNLREQQTRLQARMDELRAEIAFAQKGEKPWPYRSCWMREG
                     RGCCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIIDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITNGGRNWL
                     SFNWPSYRSRIGGVAA"
     gene            complement(4718..5626)
                     /locus_tag="ACHJZ2_001453"
     CDS             complement(4718..5626)
                     /locus_tag="ACHJZ2_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4567599.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETTRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARI
                     REGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEDFWQQPAAPGGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(5613..5846)
                     /locus_tag="ACHJZ2_001454"
     CDS             complement(5613..5846)
                     /locus_tag="ACHJZ2_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567600.1"
                     /translation="MIRRVVNSLYHRYNRCPRVGQWFTTSNGHVLRVCLVNTESQMVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGGYASV"
     gene            complement(5843..6505)
                     /locus_tag="ACHJZ2_001455"
     CDS             complement(5843..6505)
                     /locus_tag="ACHJZ2_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO4567601.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKAGAGGRSPELLEAIYDAPASF
                     LSSMLAHARIMVGWAGEPKGSPVSLSAGTANPVQFTTHEFRSSGGGSFSLLKEAAIMA
                     TVPTSPYLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTY
                     HIYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCIEAFHEAEISLSHTAFRRNQWEV
                     RP"
     gene            complement(6614..7321)
                     /locus_tag="ACHJZ2_001456"
     CDS             complement(6614..7321)
                     /locus_tag="ACHJZ2_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4567602.1"
                     /translation="MNTAIFNDKASMTSVEIAELVGSQHKDVKRSIERLMGKGVIRSA
                     PMANFEIINNLGLKRNVAAYVFEGEQGKRDSIIVVAQICPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVIHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCCNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            complement(7403..7636)
                     /locus_tag="ACHJZ2_001457"
     CDS             complement(7403..7636)
                     /locus_tag="ACHJZ2_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4567603.1"
                     /translation="MLRKLIPQNAIARYIGVTPQAVNLWFSKNSVPSRFVLRVCELVE
                     WKVTPHGLRPDLYPYPEDGIPDSLRKSNGITRD"
     gene            7793..8482
                     /locus_tag="ACHJZ2_001458"
     CDS             7793..8482
                     /locus_tag="ACHJZ2_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4567604.1"
                     /translation="MKNVKSTENRIAMMLKTKGWSQAELARKLGVSAQSVQYWTTGKT
                     FPRSDKLAQLSVISGYPQSWFLGEDASSTFSSAEKHHTREDSVVFNVLDVEFSCGDGT
                     HVRGDLIDVVRSIELDPEYARRLVGNRAFKNIEIGNARGDSMAPTISPGDLLFLDKTV
                     TYFDGDGIYAFCFDGECYVKRLQKIGSKIMVLSDNPNYQPWSIEKEGMALLYIQSKVI
                     SSVPFNINRFG"
     gene            8630..9331
                     /locus_tag="ACHJZ2_001459"
     CDS             8630..9331
                     /locus_tag="ACHJZ2_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4567605.1"
                     /translation="MEALQTTPKTCSLNTYNKVLCDDLDLDSFALTIANLLSAVRTFN
                     LLDDTRLKEVGFDVLEFTHEYALAIASTKQQHSIRSGNKIACIRTKREACGLTTTELA
                     RLLDLDEEIILQWESGEFEPTISMLIPLANVLGCDPLSLLSEKNSESAIRVNTTEVHA
                     ESIGARIKSARTKLGLTESDLARMIHTYSDPINDWECGICEVPADHIVPLASALNCDL
                     MWLLTGKSEAKEQQQ"
     gene            9328..9528
                     /locus_tag="ACHJZ2_001460"
     CDS             9328..9528
                     /locus_tag="ACHJZ2_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567606.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHNLLCAVIVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            9727..9908
                     /locus_tag="ACHJZ2_001461"
                     /pseudo
     CDS             9727..9908
                     /locus_tag="ACHJZ2_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9914..10486
                     /locus_tag="ACHJZ2_001462"
     CDS             9914..10486
                     /locus_tag="ACHJZ2_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567607.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPGPIRFALQQYIEWNLIQKYGEAEGMGRALFLYQKMLITYPDKGF
                     IVSDMGREGLELLLDEMINDLNTHGMPEGQ"
     gene            10671..10859
                     /locus_tag="ACHJZ2_001463"
     CDS             10671..10859
                     /locus_tag="ACHJZ2_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567608.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTIAVTSGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            10856..11683
                     /locus_tag="ACHJZ2_001464"
     CDS             10856..11683
                     /locus_tag="ACHJZ2_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO4567609.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITATDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGVQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKALAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGANTPFIQIK"
     gene            11724..12095
                     /locus_tag="ACHJZ2_001465"
     CDS             11724..12095
                     /locus_tag="ACHJZ2_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO4567610.1"
                     /translation="MNNQNTQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVINCAIESLYATLPYEEVLDERTGKTDIMATINIGELICQDEDLSGELW
                     LAGMLISAEIISIEPATNIRL"
     gene            12127..12231
                     /locus_tag="ACHJZ2_001466"
                     /pseudo
     CDS             12127..12231
                     /locus_tag="ACHJZ2_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            12287..12541
                     /gene="xisR"
                     /locus_tag="ACHJZ2_001467"
     CDS             12287..12541
                     /gene="xisR"
                     /locus_tag="ACHJZ2_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4567611.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            12575..13861
                     /locus_tag="ACHJZ2_001468"
     CDS             12575..13861
                     /locus_tag="ACHJZ2_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4567612.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEIGSN
                     AFSRYQSVVRNMLPRIGEKRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPIKGRSVV
                     TVNYYMTTIAGMFQFAADNGYIVSNPFNGLTPLKRSRTEPDPLTRDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAVDALKSQAEMTRLGPQYQIDVKLREFGRTARHECTFVFNPQLVKKCQQVGH
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALSAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKQLTESVPYMPHRARL"
     gene            13845..14642
                     /gene="yecE"
                     /locus_tag="ACHJZ2_001469"
     CDS             13845..14642
                     /gene="yecE"
                     /locus_tag="ACHJZ2_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO4567613.1"
                     /translation="MEPDSEIISLNISCFSGSCRHINCVEGNTTLYALPKPEVVLRWR
                     EQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPR
                     ELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVILDSRPVHA
                     ARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQ
                     TTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            14695..15090
                     /gene="yecN"
                     /locus_tag="ACHJZ2_001470"
     CDS             14695..15090
                     /gene="yecN"
                     /locus_tag="ACHJZ2_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO4567614.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            15131..15874
                     /gene="cmoA"
                     /locus_tag="ACHJZ2_001471"
     CDS             15131..15874
                     /gene="cmoA"
                     /locus_tag="ACHJZ2_001471"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO4567615.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            15871..16842
                     /gene="cmoB"
                     /locus_tag="ACHJZ2_001472"
     CDS             15871..16842
                     /gene="cmoB"
                     /locus_tag="ACHJZ2_001472"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO4567616.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(17007..19436)
                     /gene="torZ"
                     /locus_tag="ACHJZ2_001473"
     CDS             complement(17007..19436)
                     /gene="torZ"
                     /locus_tag="ACHJZ2_001473"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO4567617.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(19461..20561)
                     /gene="torY"
                     /locus_tag="ACHJZ2_001474"
     CDS             complement(19461..20561)
                     /gene="torY"
                     /locus_tag="ACHJZ2_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO4567618.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLMAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKLLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(20949..21695)
                     /gene="cutC"
                     /locus_tag="ACHJZ2_001475"
     CDS             complement(20949..21695)
                     /gene="cutC"
                     /locus_tag="ACHJZ2_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO4567619.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(21709..22275)
                     /gene="yecM"
                     /locus_tag="ACHJZ2_001476"
     CDS             complement(21709..22275)
                     /gene="yecM"
                     /locus_tag="ACHJZ2_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4567620.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            22491..24224
                     /gene="argS"
                     /locus_tag="ACHJZ2_001477"
     CDS             22491..24224
                     /gene="argS"
                     /locus_tag="ACHJZ2_001477"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO4567621.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(24266..24310)
                     /locus_tag="ACHJZ2_001478"
     CDS             complement(24266..24310)
                     /locus_tag="ACHJZ2_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567622.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            24401..24889
                     /gene="yecT"
                     /locus_tag="ACHJZ2_001479"
     CDS             24401..24889
                     /gene="yecT"
                     /locus_tag="ACHJZ2_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO4567623.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(25009..25401)
                     /gene="flhe"
                     /locus_tag="ACHJZ2_001480"
     CDS             complement(25009..25401)
                     /gene="flhe"
                     /locus_tag="ACHJZ2_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO4567624.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(25401..27479)
                     /gene="flhA"
                     /locus_tag="ACHJZ2_001481"
     CDS             complement(25401..27479)
                     /gene="flhA"
                     /locus_tag="ACHJZ2_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO4567625.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(27472..28620)
                     /gene="flhB"
                     /locus_tag="ACHJZ2_001482"
     CDS             complement(27472..28620)
                     /gene="flhB"
                     /locus_tag="ACHJZ2_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO4567626.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(28822..29466)
                     /gene="cheZ"
                     /locus_tag="ACHJZ2_001483"
     CDS             complement(28822..29466)
                     /gene="cheZ"
                     /locus_tag="ACHJZ2_001483"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO4567627.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(29477..29866)
                     /gene="cheY"
                     /locus_tag="ACHJZ2_001484"
     CDS             complement(29477..29866)
                     /gene="cheY"
                     /locus_tag="ACHJZ2_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO4567628.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(29881..30930)
                     /gene="cheB"
                     /locus_tag="ACHJZ2_001485"
     CDS             complement(29881..30930)
                     /gene="cheB"
                     /locus_tag="ACHJZ2_001485"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO4567629.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(30933..31793)
                     /gene="cheR"
                     /locus_tag="ACHJZ2_001486"
     CDS             complement(30933..31793)
                     /gene="cheR"
                     /locus_tag="ACHJZ2_001486"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO4567630.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKTLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(31812..33413)
                     /gene="tap"
                     /locus_tag="ACHJZ2_001487"
     CDS             complement(31812..33413)
                     /gene="tap"
                     /locus_tag="ACHJZ2_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO4567631.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(33459..35120)
                     /gene="tar"
                     /locus_tag="ACHJZ2_001488"
     CDS             complement(33459..35120)
                     /gene="tar"
                     /locus_tag="ACHJZ2_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO4567632.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(35263..35766)
                     /gene="cheW"
                     /locus_tag="ACHJZ2_001489"
     CDS             complement(35263..35766)
                     /gene="cheW"
                     /locus_tag="ACHJZ2_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO4567633.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(35787..37751)
                     /gene="cheA"
                     /locus_tag="ACHJZ2_001490"
     CDS             complement(35787..37751)
                     /gene="cheA"
                     /locus_tag="ACHJZ2_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO4567634.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(37756..38682)
                     /gene="motB"
                     /locus_tag="ACHJZ2_001491"
     CDS             complement(37756..38682)
                     /gene="motB"
                     /locus_tag="ACHJZ2_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO4567635.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(38679..39566)
                     /gene="motA"
                     /locus_tag="ACHJZ2_001492"
     CDS             complement(38679..39566)
                     /gene="motA"
                     /locus_tag="ACHJZ2_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO4567636.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(39693..40271)
                     /gene="flhC"
                     /locus_tag="ACHJZ2_001493"
     CDS             complement(39693..40271)
                     /gene="flhC"
                     /locus_tag="ACHJZ2_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO4567637.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(40274..40624)
                     /gene="flhD"
                     /locus_tag="ACHJZ2_001494"
     CDS             complement(40274..40624)
                     /gene="flhD"
                     /locus_tag="ACHJZ2_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO4567638.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            41404..41832
                     /gene="uspC"
                     /locus_tag="ACHJZ2_001495"
     CDS             41404..41832
                     /gene="uspC"
                     /locus_tag="ACHJZ2_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO4567639.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(41839..43263)
                     /gene="otsA"
                     /locus_tag="ACHJZ2_001496"
     CDS             complement(41839..43263)
                     /gene="otsA"
                     /locus_tag="ACHJZ2_001496"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO4567640.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(43238..44038)
                     /gene="otsB"
                     /locus_tag="ACHJZ2_001497"
     CDS             complement(43238..44038)
                     /gene="otsB"
                     /locus_tag="ACHJZ2_001497"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO4567641.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(44205..45191)
                     /gene="araH"
                     /locus_tag="ACHJZ2_001498"
     CDS             complement(44205..45191)
                     /gene="araH"
                     /locus_tag="ACHJZ2_001498"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO4567642.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVA
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(45206..46720)
                     /gene="araG"
                     /locus_tag="ACHJZ2_001499"
     CDS             complement(45206..46720)
                     /gene="araG"
                     /locus_tag="ACHJZ2_001499"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO4567643.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(46790..47779)
                     /gene="araF"
                     /locus_tag="ACHJZ2_001500"
     CDS             complement(46790..47779)
                     /gene="araF"
                     /locus_tag="ACHJZ2_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO4567644.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
BASE COUNT        12128 a        12690 c        11418 g        12096 t
ORIGIN      
        1 ttcaggtaat cctcatcacc cgtccactgg ttatcccagc cctcaggctt cactttaaag
       61 tacggcgggt ccgtgactat caggtcaaca gaattttcgg gtaacgaccg gataaattcc
      121 aggcagtcgg cgttgattaa ctcacaactg gatattttta cagtattaaa catggatcat
      181 taagcctgtc tctgataggc tcataccgct tttgcgcaaa gcagatgggc ctgaggtttg
      241 cttgtgaccc caacgcatga gcagatggct ggtgagtgcc cctaacaccc accagccgcc
      301 catttaccac aaataaaaaa gccttcagga ctgaaggcgt ctgtaacaac caaactgata
      361 gtctgccaga cccgccataa caagctgggt cagtattagc tggcagcgtt cgcgtgaaag
      421 gtacgtattc tgtgcaatct ccccgactgt cgccggtgcg gtggcactta attcattaaa
      481 caccactctg gcggtttctg tcatatcctg ctgtttcagc atgtcttttt cccttttccg
      541 gttaacgtga cacaccaata actcttgtcg aaaaagccag caagctgaaa gaccggtatt
      601 caccgccacc agcgcgttta acgtactggt ccgatttcag gcataaaaaa cccgcctgac
      661 ggcggattta agctgtgtgg caaagtaacc actcttaaca tactgacata ctttttgcgg
      721 accgcactaa tcatttttta cttttttagc agccagtcgt ccatctccag tcttaccccc
      781 agcacagaca aacatccgtc aataaaccct tcggctatct gcatctcaat tcgtattgct
      841 ttttcgcttt tctttctcgt cctggctatc tgtctttttg atattcgcaa caaataatga
      901 gcaatgagaa gcgaatactc ctcaggtttt ttctgcttca gacgtgcaag acagttttca
      961 atgataagtc cgtcatcatc gcagcaggct ggacgtggtt tagtggtaga tggtaaaagg
     1021 cctttgaatc cggcagcgat cggagaatag tccaccccgg tgttaccact tgccgcccat
     1081 gccccccagc gttcaagaac catctgaata tcacgcatca actttctcca caaaaatcag
     1141 gacagcacac caatcgccag tgcgcgatcg ataaaacgaa atatcagctc cagttgggaa
     1201 ccatacttct cttcaaatgc cacggtatcc gcatgcagtt cgtcatggtg ttttctgcac
     1261 aaaggcaaca caaaaaggtc atgcgctttt gtacccattc caccctgacc atggccaatc
     1321 aggtgatgtg gatcgtcggc tggcttacca caacacgcgc aaggctgtgt cttaacccag
     1381 cgcgtgtact tttcattaac ccagcgacga cgtttggggc gtaacataaa agactccggc
     1441 gactccggat ccactttcag cgccagcacc tttttcgctt tatcctggat aatgctggtg
     1501 gcaggaaccg aaggcacaag gtcactctcc cgggtgacag acggcaaaac aggcttcggt
     1561 aatctcaggg ccttacgggc tgcactttcc ggtaaggcat ccgccaggtc attacgaacc
     1621 agccaccagc acagttcagg cattgtcaca acgtgactgt catcaaaacc gagatcacga
     1681 cggactacgg acaacaccca gcgggcacag ttatccgttg ccattgactc cagacgttcc
     1741 gtgaactgat cgcgaagcag gttatcgcag tgccagcaca gacggattgc gcccggcgcg
     1801 tgtcgcattg tggtcatgtt ctcgctgtgc catccggaat gaggccactg gcagcctttt
     1861 tcacgaagta accagctctc aaggcattcc acgccaccag cacgacggat caccgcctca
     1921 tggcggaaca cggcccgaac ggcaggatca tccgccagcg gttgtgatgc tgccggaacg
     1981 gcaccactgg caaaagatga ataacgttcc ggctcaggct ccagcaggac acgcccctgc
     2041 ataaacaggg gcatcagctc tgaaccgggt ctgaacaaga cgatccccat acgcggggca
     2101 atttcagggg tcagcagtgc tctcacggtc acctcagcga acggtattgc atgaacgcag
     2161 gagaaaaaat tcagccatca cgcagtaaac tctttcacca gcgtttcaaa ctggcttacc
     2221 tggccttcca gttccgccac gcaatccacc agctcatcca ccgccttttg tgtgcgatgt
     2281 tttgcctgca gcagatcacg aagcgccgga gtaagctgct tgcggagcgt atcttttgcc
     2341 acgctcgttt tttccatctg ttcagcacaa cgaagcatct cctgcgcctg ccgacgaagt
     2401 tgttccggtg aaacagtggt ggttctgttg ttcaaaataa acgctccgtt ttactacccg
     2461 acatgcggtt attgctgtat ctgcgcggat tgcccggcgt catgggagtg gaaagaaccc
     2521 gggcactctc ctggtccaca ggcagaaaat gcccgttatg aaaacgccgg taaatggtac
     2581 ccagcgtgcc attacgctgt ttcgtgatgt tgatttcagc tatgcctctc gcctgtgtct
     2641 ccgggttgta cacctcatcc ctgtaaagca tcagaatgat gtctgcatcc gcctctattt
     2701 cccctgagtt tttcaggtcc gagttcatgg ggcgtttatt gggtctggat tccacgccac
     2761 gggagagctg gctcagagca atcagcggaa aaccgccgga ttttgccagg cttttaagtc
     2821 cctttgagat ttcccccaca gcaaggtcgt gacgccccgt gctgcgggtt ttaatcaggc
     2881 cgaggtaatc gaccaccacc agcgccgttt ccgggtgttt catccggtgg tgcctcgtgg
     2941 ttgcacatat ctcatcaatg gtcaggtttg cctggtccac catccagata ttacgccccg
     3001 tcattcgtcc cacgccctgt gagaaacgcg cccagtcttc atcttcaaaa cgggcaacag
     3061 acttaagacg ggataccggc attcccccgg cagcagacac catacgttca ccaatctgaa
     3121 tgttcgccat ctccatggtg aacagaagca cgccatgccc ctgctcagtc accttgtcga
     3181 tgatatccag cgcaagttcg gttttcccca tcgaaggacg ggccgcaatg aataccaggt
     3241 cgcctggctc cataccgccc gtttttgcgt ccagttcatc aataccggtc atcagcgccc
     3301 tggatttctc cagtccctga ttgcggcatt caacacggtc gaccacttcc ggaaggacat
     3361 catcaatgtg aaccggctga atgacgccct ttccggtcga cagtgaggcc atcatgttct
     3421 gcgcatcctt cagggcatcc tcggctgctt cacaggtatg cgcatcccgt aaattctgta
     3481 atgcttcagt cagtgttttt tctgcatcgc gcagtgcggc attacgccgc aacgctgcga
     3541 catagtgctc cagtgaagac ttcacccagg ttttgcgtcc ggtgtcggta atcaccgggg
     3601 caagttccgg catctcattg cacagcagta cggggtcaat gacgccggat acacgtgcct
     3661 gtctgcaaat tcccgcgtaa atatcccggt actgtcgtgc agaaaaaacg tccgccgaca
     3721 atgtggccag aatatccatc acttccggat cagccccacg cagaaaaaac gcgccaatga
     3781 cagcgccttc caggtcatcg ttacgccatg ccggggtgtt ctggctggtc atgcggcaac
     3841 acctccgata cgagaacggt agctgggcca gttaaacgac aaccagttgc gcccgccatt
     3901 ggtgatcctg tcggcaatcc ggggactgat gaacgcccac aattcttccg gtgaaaggtt
     3961 gctgatcaag atagttggca aaataccctc ataccgggca ttgataattt cctgcaaaat
     4021 ggccatttca gccgcactgc caaactgaac gccgacttcg tcgatgatca gcaaatccag
     4081 tgacgcataa tgctcaatga cgtcatccgc tgttttttca ctgtcattcc gccagcagtt
     4141 tttcacagca cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg
     4201 gatgatgtgt tttgccattg ataccgccag atgatttttc ccggtaccgc aactgccggt
     4261 cataacaaga ctggtaccgt tctccagcat atctggccag ttctccgcat agcggcgaca
     4321 ggccgcaaga tttctggctg cgtcaggatt aacctccaga taattatcaa actcgcagtc
     4381 ccgaaaacgc agagcaattc cggcgttatc agtcagttct tccgccttga gggacgacag
     4441 ctccatggtc aaatcgttgg cctcagctat caagcagtca gggcagcatg aaattttttc
     4501 tctgtcctcg ccattacgat cgctccacac cagaatatgt gtgtggtatt cgccatgttt
     4561 ttcgcaacac ccgcgccctt cacgcatcca gcaggaacga taaggccatg gtttttcgcc
     4621 cttctgagca aatgcaatct ctgcccgtaa ctcatccatc cgcgcctgta gtcttgtttg
     4681 ttgttcacgc aggttaaacg tcatcatcgc tgtcacctca gaatgtcaat ttgtcactgg
     4741 atttaccgaa tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc
     4801 gcccacctcc gggagctgct ggctgttgcc agaaatcttc gaagtgacga tcgggtccaa
     4861 agaacgtcga cgcctgcttc acgaactggg tgccggtatt tcctgagaca cgcacccagg
     4921 cagcatagcg tttcacaccg ttgagcatgg tttctggtgt cacaccttcc ctgattcggg
     4981 ctttccaggc tttgaaggct gctgacttgg aattaccacc agcacgtttg ggatattcct
     5041 gccaggcctg ttcaaattcc ggtgaatatt cctgtcgggc agaacgcgct ggtgcagacg
     5101 cgtcagcgga tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg
     5161 ttgtattctc tgtaagatcg aaattggttt tcccttctcc gcggcgagtg gtttcccgtg
     5221 tccgcggtga aggctttccc tcctccgcga aattgggttt tacagtttcc cgaaaacggg
     5281 tttccccatt tcgggaaaac tgattgtttt cattgataat ttcattaagg cgctcacaat
     5341 ctatacggta gaacattttg tgctcaagac gcttgttggt ttcaaccaaa atgcctctgg
     5401 acacaagatg cttacgcgct acagcctgtt gttcaaatgt aagtccagtt tcgtgttgta
     5461 tctcttcacg cgttttatgt acgccttccg ctgcatgtgc tttatcctgc cagtaaaaaa
     5521 tctgaccaaa gaaaataaca gcgtgcggac ttcccatgta tttaacgagc ccagggtaat
     5581 aagcaaccgg atgcccaaaa tcgagcagaa gatcagacgg acgcatagcc acctcccaga
     5641 cgcttaaaca ttttcccgga ctgaaaagcc accagcggat aactcagggt atgagtacgt
     5701 ccctgaacct ggcagacaac catctggctt tctgtattga ccaggcaaac ccgcagaacg
     5761 tggccgttgc tggtggtgaa ccactgcccc acacgggggc aacggttgta tcggtgatac
     5821 agggaattaa cgacgcggcg aatcatgggc gcacctccca ttgattacgg cggaaagcgg
     5881 tgtgactgag gctgatttct gcctcatgga atgcttcaat gcagctctcg tagtaccgca
     5941 ttgtgcgtag acttaaccca agctgaagca tcatcagacc atcaagggtg atgtaataac
     6001 cacgcaggga gtcaccgtag atgtgataag tacccggtat gaaattgcgg gtaaaaaact
     6061 cgcgtgagca gttcagatac tcgattttgt cgacgatgtt ctggtgcatg cgcttaaagt
     6121 ggcaggcaac atgcagggag aaaataacgg ccttgccgtt gacaacttca attttcaggt
     6181 atggggaagt tgggactgta gccatgatgg cagcctcctt gagcagtgaa aaacttccac
     6241 caccggagct gcgaaactca tgggtggtga actgaacagg gttcgcagta ccggcgctca
     6301 aggaaaccgg cgagcctttc ggctcccctg cccagcccac cataattctg gcgtgcgcga
     6361 gcatggacga taaaaaagac gctggcgcgt cgtatatcgc ctcgagcaat tccgggctgc
     6421 gacccccggc acccgcttta taaggtgcgg agacagtgta acgtcccgaa attgcagaat
     6481 caatatttgg tcttgaaatg atcatatagc tgctgatatc tttagaactg ttcttggatg
     6541 tttcggagcc gttttatgcg aaacagctcc ccgttattga tgttgagtga gccgggttac
     6601 tcccggcttt ttttcaccgc tgccaaccaa taacctgaaa taaccccatt ttcgggtgat
     6661 accagcgagt ccctcgcggt tctgcttcct ccataacccg ataaaaagca gccataaacg
     6721 gttccacagc aacaattgcg cgacgtgaca acaatccgtc cggcgtcatg aactcatgtg
     6781 tgtctgtagg aatttgatag gcgttcacca gattgcagca tttatcatct gacaaaccgg
     6841 tttttgcttt cagttggcga tatccggcat agccctcacg aatagtgccc tttttaattt
     6901 gctcaactgt ttcagcaacg tggatgactt tttcttccac ctgagtgatc cgtttctgtt
     6961 ggcgaacggc ttcaagagcc atcgcggcaa ccatttcgat ttcgctcatt ggcttacgga
     7021 tctgttcttc cagttcacgc cagcggtcta ccaagcgggc ggtgaattca gggcaaatct
     7081 gcgcgacaac aatgatactg tcgcgtttgc cttgttcacc ttcgaatacg taagcagcta
     7141 catttctttt caatcctaag ttgttgatta tttcgaaatt cgccattggc gcacttcgga
     7201 taactccctt ccccatgagt ctttcaatgc tgcgtttaac atctttgtgt tggctgccca
     7261 ccagctctgc aatctcaacg ctggtcatgg atgctttatc gttaaaaatt gcggtgttca
     7321 tctgattgct ccttgaggat gacatttcag aactcacgcc aaaacgtatt catcgtgaat
     7381 tactgcttgt gacagtcagt ctttaatctc tggtaatacc gtttgatttt cgtaacgaat
     7441 caggaattcc atcttcggga taagggtata gatcaggtct taacccatgc ggagtaacct
     7501 tccattcaac caattcacac acccgtaaaa caaaacgaga aggaacggag tttttagaaa
     7561 accacagatt caccgcttgt ggcgtaacac cgatgtatct cgctatggcg ttttgaggga
     7621 tcaatttacg caacatgtcg taatcattca gttttatcac agcgcagctc caacattaac
     7681 tttacaaatc aagaatacat caagaataaa ttaacatgca agtttcaaaa ggatcgaata
     7741 cactaaaatc aagttaatat ttatgtgtat aaagcttcga caggaactca ccatgaagaa
     7801 tgtaaaaagc acagaaaatc ggatagcaat gatgctgaaa acgaaaggct ggagtcaggc
     7861 tgaactagcc cgtaagctgg gtgtaagcgc gcaatcagta caatactgga ctacaggaaa
     7921 gacgtttccc agaagcgata aacttgcaca gctatcagta attagtggtt atccacaatc
     7981 ctggtttttg ggtgaagatg cctcatcgac attctcttca gctgaaaaac accatacaag
     8041 agaagacagc gttgtgttca atgtactgga tgttgaattc agttgcggcg acggaactca
     8101 tgtacgggga gatctaattg atgtagtacg ctcaatagaa cttgatcctg aatatgcccg
     8161 ccgcctggtc ggtaacagag cattcaagaa catagaaatc ggtaacgcca gaggggacag
     8221 catggcccca acaatctccc cgggagactt actgttttta gataaaacag taacttactt
     8281 tgacggcgat ggcatttatg cattttgctt tgatggcgaa tgttacgtca aacgacttca
     8341 aaaaattgga agcaagatca tggtcttatc agacaatccc aactatcagc catggagcat
     8401 cgaaaaagag ggaatggcgc tgctctatat ccagtcaaaa gtaatctcat ctgtaccatt
     8461 caatatcaac agatttggtt agttattgat tttaaattaa attattggtc atccatcaca
     8521 aaaaaatcaa gtttatcaat ttttaattga cagtcgtttt cctgacacat aatatctcac
     8581 catcaattat atattgatta acttcaactt agaattgcat ggtgatgata tggaagcctt
     8641 acaaacaaca ccaaaaacat gtagcctcaa tacttacaac aaggttttat gtgatgattt
     8701 agatctcgat tcttttgcat taaccatcgc aaacctgctc agtgctgttc gcaccttcaa
     8761 cctcctagat gatacgcgat taaaagaggt tgggtttgat gtgctagaat ttactcatga
     8821 atatgcttta gcgatcgcat cgacaaaaca acaacattct atccgatcag gcaacaagat
     8881 agcctgcata cgcaccaaac gtgaagcctg cggcttgacg actaccgaac tagccagact
     8941 actcgatctt gacgaagaga ttattctgca atgggagagt ggagagttcg agccaaccat
     9001 cagcatgctt atccccctgg caaacgtcct gggatgcgat ccgctttctc tactaagtga
     9061 aaaaaacagc gagtcagcta ttcgtgtaaa tacgactgaa gtccatgcgg aaagtattgg
     9121 cgcacgcatc aaaagcgccc gtacaaagct gggattaacc gaatctgatc ttgcccgcat
     9181 gattcatacc tacagtgacc ccataaacga ctgggaatgc ggcatctgtg aagttcctgc
     9241 tgatcatata gtaccactgg ccagtgcgct taattgtgac ctgatgtggt tgttaacggg
     9301 aaaatcagaa gcaaaggagc agcaacaatg actggcaata tccatgataa gtatgaaggc
     9361 ttatgcctgg caccggattc ctttgcaaac aatatccata atttattatg cgcagttatc
     9421 gtattacaaa tgtcagacaa cgacgcaata aaaagaacag gtgatgaagt tcttgaattt
     9481 gcacgttgct atgctgaagc agcagctgaa aaagaactat ccagttaaat agaacaagtc
     9541 atctccagat actatattac ggcttaatcg ccggggatta tcacacactt aatccacagg
     9601 aggttttatt atgactttta taaaacataa ggcatcacac aaaacagcct gccttattgc
     9661 acaacacggg aaaaatcaca tgcatattgc ctgcttgttt ctgcgtaaag catacgggag
     9721 ataataatgc atcagaaaac agcagaacac gaacaaacca gagtattgct gaccatcaaa
     9781 aacgggaaag taatattaat tcgtcatgtt catgacgatg aatttgtaga aatctttcaa
     9841 cattcctgtt tattgcagaa aaggcaggat atgacgttat tgcaccagca gatgaagatg
     9901 aggaataaat ttcatgcaat acgctgaatt ccaggctgaa gcaacagcca atggtataca
     9961 aacaggcagt atgactattg attatcacga cgccatacgc cgtctggatg ctggagaatt
    10021 cgatactcct aatgtgcgag gtttacgtat ccttcagtgt ctcgcgcaag ctgacgaagc
    10081 aggattactg ggaaaacttc cggttgagat gaaggttgcc cagtggcgat ggttgtatgt
    10141 gacgacattc atcaacgaag aagaagacaa gaacggcaca attgatgtcc cgaatgaaca
    10201 cggaacaaca gatcgcgccg taatatataa cgggaagcat ggggttatga cgatatatcc
    10261 cggccccatt cggtttgcct tacagcagta tattgaatgg aatttaattc aaaaatacgg
    10321 cgaggcagaa ggaatgggta gagcgctgtt tctttatcag aaaatgctca ttacataccc
    10381 agataaaggt ttcattgttt cagacatggg gcgagaaggg cttgaactcc ttctggatga
    10441 aatgattaac gacctgaata ctcatggtat gccagaaggc cagtgacaca ttaaatatta
    10501 agaagaatat aattcttccg ttttttacta accatttata tgaaaaacaa ccgtgaatta
    10561 agcagagtaa aactgctttt aatccttgcc acagtactga caataacaga aatcattatt
    10621 ctctttattg cgctgtctgt cagttaaaaa tatcgggata ccacatacca atgagactgt
    10681 atttcacaat agtaatttta ctggcaatta tcgcatgcat ttacggatta ctcgttccgt
    10741 tccttatatc catgaaggat acgatagcag ttacttctgg ctttgcactg gcgtttctga
    10801 ccccgccctg catttatgcc atttacaagg gtctttcttt cactaaggat aaaaaatgaa
    10861 aaaaattatt tttgctttag ccattgttct gccgaccatt ggccttgtcg gctgcgatcg
    10921 cgttgaaccc ggtaatgttg gcatcaaagt aaataaactg ggcgacgata aaggcgtcgg
    10981 tgaggtggtc ggtgttggtc gctactggac tggctggaat actgaagttt acatcttccc
    11041 aaccttcaaa caaatgaaga cctacgatga gccgttcagc ttccagatga gtgacggcac
    11101 aaccatcggc tatcacatcg gtgttgccta caaagttgat ccatccaaag ttaccacggt
    11161 gtttcagacc taccgcaaag gcgtggatga cattaccgcc actgacctgc gccagaagat
    11221 cgccgacgca ctcaatcgac tggccagcaa aatgaccacc gacaaattta tcgacggcgg
    11281 caagtctgaa ctactggatg ccgcacttaa agacattcag gaagagatga cccccatcgg
    11341 cgttcaggta atgagcctct cttatgtagg taaaccggaa tacccgccaa ccgttatcga
    11401 cagcattaac gccaaagtca cggcaaacca gaaaaccctg cagcgcgaac aggaagtcaa
    11461 gcaacgtgaa gcggaggcca acatgctgcg tgcagaagct gccggacagg ctgatgccat
    11521 tcgaacaaaa gccctggcag aagccgatgc cattcgttta cgcggcgaag ctctgcgcca
    11581 gaaccctggc gtcatggagc tggaagccat caacaagtgg aacggtacac tgccgcaata
    11641 catgaccagt ggtgccaata caccatttat ccagattaaa taacttacac gcccggcaaa
    11701 ccgccgggtt aatggaaaat cagatgaaca accagaatac tcaaccgcaa ataatgaact
    11761 atgacccgaa tctgacgtcg tgcggacgca tggcaaaaca aaccgttcga ttaactttcg
    11821 gactatggga ataccgcgaa acattcgaag ttactgtcgg cggcaatctg accggactgg
    11881 atgttatcaa ttgcgctatt gaaagcctgt acgcaacgct gccttatgaa gaagtcctgg
    11941 atgagcgcac agggaaaacg gatatcatgg ccaccattaa tattggcgaa ctgatatgcc
    12001 aggatgaaga cctgtccggg gaactctggc ttgccgggat gcttatctca gcagaaatta
    12061 tcagcattga acccgctaca aacatacggc tctgaagttc tcactattca gagagcagga
    12121 gaaaaaatgt tcgctctgat taatcaggga caactgtata ccgacagtgc cggttacccg
    12181 gtaaaaattg ttcgctgcat aaacaacacc gtgttgtaca gaagatgtga agcgaatgtt
    12241 tgaagtgtgg tgccactgcc gtggatgaaa gttttatgag gttggcatgc agacaatcat
    12301 ctatcagata acccccagca aatggtgtac ggagagagtc ctcattgcat caacagggct
    12361 aaagcctggc accatcgagc gagccagaag aaagtcatgg atgcagggaa aagaataccg
    12421 ccattacgct gtagaaggtg atccggggca ttacagtgaa tgcctgtaca acatcgaaga
    12481 aattatgcga tggatcgaaa accagaaaca accaggtgcc aaaaatgcaa gttccggtta
    12541 acctgttaat gctcctggac gtctgggagg ttttatgagt aacgcatcat acccgacagg
    12601 cgttgaaaac catggaggat cactccgtat atggtttcac tataatggca aacgtgtcag
    12661 agaaaacctc ggtgttcctg acaccgccaa aaaccggaag atcgctggtg agcttcgcac
    12721 ttctgtttgt tttgcaatca gaatggggag tttcgactac gccgcgcagt tccctaattc
    12781 ccctaacctg aaacactttg gtctgggaaa aagagagata accgttaagg cactttcgga
    12841 aaaatggttg gaccttaaga aaattgagat aggaagtaat gcattcagtc ggtatcaatc
    12901 cgtggtgaga aacatgcttc ctcgcatagg ggaaaaacgt cttgcttcgt cggtaacaaa
    12961 ggaagattta ctgtttatca ggaaagattt attgaccggg tatcataatc tttctaacgg
    13021 aaaaacaacg ccgattaaag ggaggtcagt agttacggtt aattactaca tgacgacaat
    13081 tgcaggaatg tttcaatttg cggctgataa cggctatatc gtgtcaaacc catttaacgg
    13141 cctgacacca ttaaagagat ccagaacaga accagatccg ctcacacgtg acgaatttat
    13201 tcgttttatt gatgcctgtc accatcaaca aacaaaaaac ctgtggtcct tagcagtata
    13261 cacaggcatt cgtcacggtg agctaatatc tctcgcctgg gaggatattg atttaaaagc
    13321 taaaacaatg actatccgtc gtaattatac aaaactcggg gagttcactc taccaaaaac
    13381 agaagcggga actgatcgtg ttattcatct tgttcaacca gctgttgatg ccctgaaaag
    13441 ccaggctgaa atgacaagac ttggccctca gtatcaaatt gacgtcaagc ttcgggagtt
    13501 cggtcgcacc gcacgccatg aatgcacgtt tgtttttaat ccgcaactgg taaaaaaatg
    13561 ccagcaagtc ggtcaccact ataaagcaga ttccatcaga gattcctggg catctgcatt
    13621 aaggcgagca ggactgcggc acagaaaagc ctatcagtct aggcatactt atgcctgctg
    13681 ggcattatcg gcaggagcga atccaagctt catagcaaac caaatgggcc atgcaaatgc
    13741 acaaatggta ttcaacgttt acggagcatg gatgaaagat aacaatatcg ggcaaataga
    13801 actactcaat aagcagttga cggagagtgt cccatacatg ccccatagag ccagactctg
    13861 aaataatatc tttaaatatc agttgtttta gtggtagttg ccgtcacatt aactgcgtgg
    13921 agggcaacac cacgctttac gccctgccga aacccgaggt tgtcctgcgc tggcgtgaac
    13981 agaccacaga tgacttccgc ttctgtttta agtttccggc gaccatttcg catcaggcag
    14041 cattacgaca ttgcgatgat ttagtgactg aatttttgac ccgcatgtca ccgttggctc
    14101 cgcgcattgg gcaatactgg ctgcaactgc ctgccacatt cggcccacgg gagctgcctg
    14161 cgctttggca ttttctcgat tctctccctg gcgaatttaa ttatggtgtg gaagtccgcc
    14221 atccacagtt tttcgccaaa ggggaagagg aacaaacgct taatcgcggt ttacatcagc
    14281 gcggcgttaa tcaggtgatt ttagacagcc gcccggttca tgcagcacgt ccacacagtg
    14341 aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct gtactgacgg
    14401 cgacaaatcc gctgatccgt tttatcggta gtgatgatat gacgcaaaac cgggaattat
    14461 ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac cactacgcct tatctttttt
    14521 tacatacgcc agatattgcc caggccccgg aactggtaca taccctgtgg gaagacttac
    14581 gtaaaacgct tccagagatc ggagcagttc cggctattcc acagcaatct tctcttttct
    14641 gaatttgcca cctatcatag acaggtgcca tcggccattt taaagggagt ttgtatggta
    14701 agcgcgctgt atgccgtttt aagtgcgttg ttattaatga agttctcttt tgatgtcgtt
    14761 cgcctgcgaa tgcagtaccg cgttgcctat ggtgacggtg gttttagcga actgcaaagc
    14821 gctattcgca ttcatggtaa cgcggtggaa tatattccca tcgcgattgt gctgatgctg
    14881 tttatggaaa tgaatggcgc agaaacctgg atggtgcata tttgcggcat cgttttgctt
    14941 gctggtcgtc tgatgcatta ttacggtttt catcaccgtc tgttccgctg gcgacgttcc
    15001 ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg
    15061 ccctgggagt tggttttctc cctgcgttag cgcacaatac gccactttct ttttcccgga
    15121 tttttacgtt atgtctcacc gcgacacgct attttctgcc cctatcgcca gactgggcga
    15181 ctggaccttt gatgaacggg tagctgaagt cttcccggat atgatccagc gttccgttcc
    15241 cggctattcc aatattattt ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg
    15301 tacgcaggtt tacgatctgg gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa
    15361 cattcatcat gataattgca aaattattgc catcgacaac tccccggcga tgattgaacg
    15421 ctgccgtcgt catattgacg cctataaagc ccctacgcca gtagacgtca ttgaaggtga
    15481 tattcgcgat atcgccattg aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt
    15541 cctggaacct tccgagcgcc aggcgttact ggataaaatt tatcaagggc tgaaccccgg
    15601 cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat gccaaagttg gtgaactgtt
    15661 gttcaacatg caccacgact ttaaacgtgc caacggttac agcgaactgg agatcagcca
    15721 gaagcgcagc atgctggaaa acgtgatgct gaccgattcc gtggaaaccc ataaagcacg
    15781 cctgcataaa gccggttttg agcatagcga gctgtggttc cagtgcttta actttggttc
    15841 actggtggca ttaaaagcag aggacgctgc atgatcgact ttggtaactt ttattctctg
    15901 attgccaaaa atcatctttc acactggctc gaaacgctgc ccgcgcagat tgctaactgg
    15961 cagcgcgagc agcagcacgg gttgtttaag cagtggtcaa atgcggtgga gtttctacct
    16021 gaaattaaac cgtatcgtct tgatttattg catagcgtta cggcagaaag cgaagagcca
    16081 ctgagcgcag ggcaaattaa acgcattgaa acgctgatgc gcaacctgat gccgtggcgc
    16141 aaagggccgt tctcactgta tggcgtcaac atcgataccg aatggcgttc cgactggaaa
    16201 tgggatcgtg ttctgcccca tctttctgat ttaaccggac gcaccattct ggatgtcggc
    16261 tgcggcagcg gttatcacat gtggcgcatg attggcgcag gcgcgcacct cgcggtgggg
    16321 atcgacccta cgcaactgtt tctgtgccag tttgaagcgg tgcgtaaact actgggtaat
    16381 gatcagcgcg cgcatttgtt accgttaggt attgaacaac ttccggcact gaaagccttt
    16441 gataccgtct tttcgatggg cgtgctttat catcgtcgtt caccgctgga gcatctctgg
    16501 cagttgaaag atcaactggt caatgaaggt gaactggtgc ttgaaacgct ggttattgat
    16561 ggcgatgaga acacggtgct ggtgccgggc gatcgttacg cgcaaatgcg taatgtctat
    16621 ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt tgtagatatc
    16681 cgcattgcgg atgtgagcgt taccactaca gaagagcagc gacgcactga atggatggtt
    16741 accgagtctc tgtccgattt cctcgaccca catgatccga gtaagacagt tgaaggttat
    16801 cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt aaaggtctgg tattactgcc
    16861 ggatgcggcg tgaacgcctt atccagccta caaaaccttg ctaattcaat atattgcagg
    16921 gactatgtag gcctgataag catagcgcat caggcagctt tatgtttgca taacctcagc
    16981 gcccgtttcc gggcgctttt cacgtcttat gcctgaacag ctggctgatc aaacgccgtt
    17041 aacttcggtg cgttacccgt atatttttcg atatacacca gcgcagagtt accggcacag
    17101 gcatttgcca gctgcgagct ggggatatcc gccgttaaca cgttcgcact gccgttttta
    17161 cacaagccat tttccagatc tggccatgca ccttcatgca cgcataccac gccttttttg
    17221 atcccgtcag tcaccacagc gcctgtcaaa atctgaccac gtttgttcca gacacggacc
    17281 agatcgccat tcgcaatacc aaagcgagca gcatcttcgg tgtgaatagt gattggttca
    17341 cgatcggcaa ccgcatattt tttacgcagt tgcgcatagt taagctgact gtgtaaacgg
    17401 tgtgccggat gtgcggtcag aagctgtaac tgcttctcgt cggcggtacc tttccactca
    17461 tcaggcgcaa gccaggttgg gtgtgccggg caatccttat agccaaattt ttccagcgtt
    17521 ttggagtaaa tctcaatttt gccgcttggc gtacccagcg catttttcac cggatcggcg
    17581 cggaaatcac cataacgaac gtactgttcg ttcttctcgc tgcggcgcat ttcgatcagt
    17641 ttattttgct gccagaaggc attaaacatt ggcatagtga cgcgttgcgc acgggcacct
    17701 ttctgagcgg catcatagaa aaatttcagc cacgccattt catctttacc ttcggtatag
    17761 atctcttttc cgcctggttt cagtaattcc gcaagttcgg caaacacgtc aaaatcgttt
    17821 cgcgcttcaa attgcggagc gacagcctgc ttcatcggta caatatgctg gttgctgtaa
    17881 tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg tgatcggtaa tacgatatct
    17941 gcatgtttag cagctgcggt ccagtagcat tcagaaacga cgatcatctc cggtttctgc
    18001 catgctttga tcagacgatt agtatcctgg tgatgggtaa agttaccgcc acccgcccac
    18061 cagatcattt tgatattcgg atacgtttgt tctttaccgt tatgctgata tttgccgcca
    18121 ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgtcattcc gccatcatca
    18181 gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac gccgccaaca
    18241 cgcgtcgggt tgccaccgtt ggagtaatga taagagaaac caaaaccgcc gcccggtgta
    18301 ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat
    18361 tgctggcgct gaattcccca gcctgccatc agcattgtgc gattcgccgc catcagttca
    18421 gcgagtttga ctatctgcgc ttccggtacg ccagtaattt ccgccgtcca ggccgcgctc
    18481 ttcggagtat tatcgctctt acctgtcaga tactcttcaa actgcggata cccggtggtg
    18541 tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct
    18601 aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt atagccaaag
    18661 aactcgatgg tttcagagcg gataggatca atggcaatca ctggtttgcc agattttttc
    18721 agctgatgga agtattccag cccttgctca tcggtactgc tccaggcaat ttttaaggta
    18781 ttcagcgggt tcattcccca cagcaccaca acctggctgt tttccagaat cagcggccag
    18841 gaggtctgct gttcatacac ctctacagaa ccgaccacat gcggcatgat cacctgtgct
    18901 gccccggttg aataatcgcc gctatgcccg gaataaccgc ccgccaggtt catataacgt
    18961 tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc gccagccgta agatccggca
    19021 aaaatggccg atggtccgtt agctttacga atacgctcat gttgctcatg aatcagtttt
    19081 aatgcctgtt cccagctcac ctgtacatag gtatcttcgc cacgaccttt cgccggttgc
    19141 agtggattgt cgagatagct ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt
    19201 acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc
    19261 gaagaaacaa tcttgccgtc cttcacttct acgttcatcg ctccccaacg tcccgcggtg
    19321 aggattttac cgcccttctc ttctgcccat gcgggtagcg gtgctgccga tgtcaccacc
    19381 agcgctccag cggcaatacc gctgtgttta ataaattcac gtcttgttaa tgtcataact
    19441 tcctccctga tcaacgagga tcactgtttc tcggtaatat ctttggcgtt gtactggaaa
    19501 taccgcgtta aaatgtccag ttcgttttcg ctcatgctgg ttcgtgcccc cattcctttg
    19561 gcaatggacg gccacgcatt gacggtgtaa tggtcggcgg caataggggc atgacaacca
    19621 gcgcaatagg tatcgtcaag tttttcagcg tattgccata gcagtttacg gtccgctaat
    19681 gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc attgattgcc gtattcgtca
    19741 gcctgccatt ccccgtttac cgtgagcgcc ttgatacctt cttcacttaa tgtggctagc
    19801 gccagccgtt gacctgccgc caggtagagc gtgttttcac tgccctgcat ttgataaccc
    19861 tgcaacagaa cgatcggctg tttgccactg gcatcaacga cggtgagatc ggtaccagga
    19921 ttcacggtag ccagctcgcc tatgcgagaa gttttgaaag gataaatatg tgcgccatta
    19981 gtcactgaag tagcggcctg actttccagc tcatgcgccg cgttgtcatc catttttatt
    20041 tctggcggaa aatgggcaat gcctttatga caatcgatac aggtttcgcc gcccttttgt
    20101 gctttgttat gcattttctg cgcagattca ctttgcgagg caatatccat ggcatcaaaa
    20161 gaatggcaac tacggcacgt tgcagagtca gttgctttta attctttcca tactgtttcg
    20221 gccatttcct ggcgatgagc ttcgaactta tcgtcactgt ctattttgcc gctaacaaat
    20281 tcatgataaa tatctttaga tgcttttaat ttagcgaata aataatccat ccctgacttt
    20341 ggaatatggc aatcggcaca ttccgcacgt atccctttct ggttcgaaaa gtggacagtt
    20401 ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc
    20461 gacgttttat gtaaggcttt ttgcgccatc aagaacccgc caccaccaac cacaaccgct
    20521 atcagcaaaa acaataaccc aatgcgtttt ttccctcgca tagattcctc taattaaatt
    20581 ccagaacacc gaaatcattg tataaataaa tacacaacga attgactact tgcttaattc
    20641 ggcacaacac tgcctgaaat aatcgctaaa gaatatgatt tattacaatg taatcattaa
    20701 ttgctaagga ataatccagt tgttatttag aatatatatt tgtcagtttt agcaatttaa
    20761 atcacataat taatgtgaat atataaaata tttttagcac aacgctatgt gcactgtcac
    20821 ccgctcaaca gatcgcggta atcgttcatt ttttatacta ttcagggata aaaaaaggcc
    20881 cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
    20941 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
    21001 ccgtctacga cataacgcga atactcgtcc gcgtgtgcat ctgatgacat ggacaatcct
    21061 tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc
    21121 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc ggccataatg
    21181 attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct
    21241 gatttttgcc ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta
    21301 tttagagggt tagcgcacat atcaaaggcg cgatgaaaag tcactgccag cggaccggca
    21361 gcagccatta ttttttccat tcgtgacata tcgacattac cgtcaacatc gagcacgccc
    21421 gtcacgagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca
    21481 aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg
    21541 atcgtcaccc gctggcgcac ggatttcagt acacccaacg acggcgttaa gcccccctct
    21601 tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagcgcgcat
    21661 tccatgctgt aacagcaaat ttccagtaat gccatttttg ctccttaatt acgccgactg
    21721 ctcgctggca acaatctctt caatggacca cggatgaaat ttaatggtcg ttttgccatc
    21781 ggtgaccgcc agcgtcggat tcggtaatcg ttcatgctca ccttttggcg aactggtttt
    21841 aactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt
    21901 cagcgtttct ggatcacccg gcaaaacgat ttcaatatgt tcccaacctt cgtgcgggta
    21961 acgtttttcc ctaggccacg gtagctccac aatagaaaac tgccagtgtg caacctgtac
    22021 cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatatttt ctgacaaaag
    22081 ctcgccacac tgttcaaacc cgcgacgcca gcgctcagca gtggcgtttt gatggcaacg
    22141 caaagaaatg tgatcggcag tcagcggagt gatattcaac cccagacggc gggaaagttc
    22201 atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga
    22261 ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc
    22321 cctgcgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata
    22381 cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct
    22441 ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc
    22501 aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
    22561 gcgaaccgca ggttcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca
    22621 tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga
    22681 ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca
    22741 acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc
    22801 gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg
    22861 tggcgaaaga gatgcacgtc ggtcacctgc gctctaccat tattggtgac gcagcagtgc
    22921 gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
    22981 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa
    23041 tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg
    23101 aagagtttgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt
    23161 tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccag atcacttacg
    23221 atcgtctcaa cgtgacgctg acccatgatg acgtgatggg cgaaagcctc tacaacccaa
    23281 tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg
    23341 cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
    23401 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc
    23461 gttatgaaac actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac
    23521 acctgatgca ggcatgggct atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc
    23581 tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg
    23641 cgggtggtac agtgaaactg gccgatctgc tggatgaagc cctggaacgt gcacgccgtc
    23701 tggtggcaga aaagaacccg gatatgccag ccgacgagct ggaaaaactg gctaacgcgg
    23761 ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
    23821 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgcat
    23881 acacgcgtgt attgtccgtg ttccgtaaag cagaaattga cgaagagcaa ctggctgcag
    23941 ctccggttat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg
    24001 aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt gcttacctgt
    24061 acgatctggc cggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa
    24121 acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctgaagc
    24181 tgggtctgga tacgctgggt attgagactg tagagcgtat gtaatcgatt tttcgtgaga
    24241 gtgaagcctg atcaggttta gccgatcagg cttttttatt accatctaaa tgtattctga
    24301 aaataggcat gccattgttt tctcgctgtt gagtaagagg ccagaagcgt aatatccagc
    24361 tccagggaaa cggtaacggt tcaatttaag gaataccgca gtgtttaaat ttcttgtatt
    24421 aacattaggc attatctctt gccaggttta cgcagaagat acgcttatag taaatgatca
    24481 tgacatttca gccatcaaag attgttggca aaaaaattca ggtgatgata ctgacattaa
    24541 cgtgatcaaa tcatgtctgc gacaagaata caatctcgtc gatgcgcaat taaataaagc
    24601 ttatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacctga
    24661 tactgaacaa cttaacttgc ttaaaaaatc acaacgagcc tggctggatt ttagggacaa
    24721 agaatgtgaa ttaatccttt caaatgagga cgttcaggat ttaagtaacc cttattctga
    24781 atcagaatgg ctatcatgta tgatcataca gaccaatacg cgaactcgcc agttgcagct
    24841 ataccgtaac tctgaagatt tttatccaag ccctttgaca agaggataat tcacatattt
    24901 ttggcatgtt ttgttgcaag ctattcctga taaatgattg caacaaaaca tccagccttt
    24961 ttcactgagt tattaaacat actcgcgagc gcgtaatttt tttgtccttc agcgataatt
    25021 cacaatcact tcattacgtt gtactttcag cgacggaatt aaccgcccac cgccaggcac
    25081 ttcccagata aagcgcaacg gttctgctgc cgctataccg gaaaaggcca cggtggttcc
    25141 gctctgccca tctaattcga cacaacgaga ttgcgaacac aagcgaaccc gcagtcctgc
    25201 aggtgtcggg ccgataagct gataccgcca cgccaccagc gtcatcaatc ctgaagcagg
    25261 ttgtcgcgta gaaagaggtg cagacgacat cgactcaccg cgatgattta gcgtaattcc
    25321 gacactactt gcctgccaca tcccctcccc ggcggcttgc accagcagcg gaaacaataa
    25381 tattgctaat aaggttctca tcatttaccg ccaattgtcg ccgtcatgcg gatatgtcgg
    25441 ttatcagaca gttccagatt cgacagcacc actaactgcg gcaagctgcg gcgcagaaag
    25501 cgagacaata atggtcgcag cgcgtggttc accaataaaa ctggcggcgc acccaacatc
    25561 tcctgacggg atagcgcttc ctgagtttgc gccagtaaac gatccgccag ccctggctcc
    25621 agtccgcccc cgccctgcaa cgcctgtagc aacaaacgtt ccagcggtgt atcgaggcca
    25681 ataacatgga cttcatcttt gccaggaaac cactgctggg taatcgcccg tcccaacgcc
    25741 acgcgcacga cggcggttaa ttcatgtgga tcgctttgga tgggcgcatg ttccgccagc
    25801 gtttcgagaa tagtgcgcat atcgcgaatc ggtacttttt catcgaggag attttgcagc
    25861 actttatgca gtgtggtgag cgtgacgacg ccagggacga gatcttccgt cagctttggc
    25921 atctcctggg cgacgcgatc cagcagctgt tgcgcctcct gacgaccaaa cagctctgcg
    25981 gcatgctggc taatgaggtg gttaagatgc gttgctacca ccgtgctggc ctcaaccact
    26041 gtgtacccct gaatctgcgc ctgttctttt agcgcacttt caatccagat agcattcagg
    26101 ccaaatgccg gatcgacggt cgcctcacca ggtaacgtcc cggcagcggt tccagggtta
    26161 atagccagcc agcgccccgg ataagcatca ccactgccta tctccacgcc tttcatcaaa
    26221 atgcgatagc gggcaggttg cagatccata ttgtcgcgaa tgtgcaccac tggcggcaga
    26281 aatcccatct cctgggcaaa tttcttgcgg atactgcgta tacggcccaa caactcacca
    26341 tcctgctgga aatcgaccat cgggatcagt cgataaccca cttccattcc cagagaatct
    26401 tccagttgta catcgttcca cgtcgcttcg acaacggcat tattctctgc catttttacc
    26461 ggttttggtt cggcaggcgc tttttgttcg cgtccacgga tccaccaggc cagccccagc
    26521 aatccggcag tgaacagcaa aaataccagg ttcggcattc caggcaccag gccgagtaaa
    26581 ccgagcacgg cagcgcttaa caacataacg cttgggttac tgaaaagctg attcaccatc
    26641 tgctcgccaa catcctgatc ggtgctgaca cgcgtaacaa tgacccccgc ggcggtagaa
    26701 atcaccagag ccgggatttg tgccaccaga ccgtcaccaa tggtcaagag cgtataactt
    26761 tccgccgcgt gtcccatgct catgccatgt tgcagcacgc cgaccagcaa cccgccgaca
    26821 acgttaatga ccatgatgag gatcccggcg atggcatcgc cgcgaacaaa cttacttgcc
    26881 ccgtccattg agccgtaaaa atcggcttcc tgagtgactt cggagcggcg ttttttcgcc
    26941 tcatcttcac caatcaatcc ggcgttaagg tcggcgtcaa tcgccatctg cttacccggc
    27001 ataccatcga gaacaaagcg cgcacccact tctgcgatac gcccggcacc tttggtaatg
    27061 accataaagt tgatgatcac gagaatgaca aacaccacga taccgatagc gaaattgcca
    27121 ccaacgagga agtgaccgaa cgcttcaacc accttccctg ccgccgctgc gccggtatgc
    27181 ccttccatta aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa cagcgtggta
    27241 aacaacaaaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa catcgccacc
    27301 agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag tatgaatgcg
    27361 ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc ggcaaggatc
    27421 tgccattgtg tcgatttcag gtttgtgggc aggcgcagca tcgcggccag attactcatg
    27481 ggtcggtttc tcgttaataa aatccagggc ttccggcacc ggaagatgag taggttgtac
    27541 agggcgctgt ccaccagcca gacgccagcg tttcagttgc cagacccagg ccagcacttc
    27601 cgccaccgcg gcgtacagtt gacccgggat ttgttgacca atctccacat gtcgatacag
    27661 cgctcgcgcc agcggcggcg cttcaagcgt cgggacgtta ttttcagcgc caatttcacg
    27721 aatgcgcagc gcgaccagcc ctgcaccttt agcgaccact ttcggtgcgc tcattttgtt
    27781 ttcgtcatac tgcaacgcta ccgaatagtg ggtcgggtta ttgacaatga catccgcttt
    27841 cggcacatcg gccatcatcc gacgccgtgc ggcagctcgc tgcatctgac ggatccgccc
    27901 tttaacatgg gggtcgcctt cgctttgttt gaactcatca cgaatatcct gccgcgacat
    27961 tcgcagcttt ttcaggtggc tgaagatttg gaaaaagacg tcaaatccca ccattggaat
    28021 gacaccaagc accaccagca gtgcgcatag ccctaccaaa tccatcgcat tacccatggc
    28081 ggtaatcgga gactcggcca ttaagcgcat catatgcggc cagtgatgcc agagaaaaaa
    28141 ccccgtcacg ctgccaacca ggatggtttt caaaattgct ttaagcaact ccgcgccagt
    28201 ctgtgccgag aacatccgtt ttatgcccgg tagcgggttg agtttggaaa acttcggctg
    28261 caaggatttt ccgctaaata ccagcccccc cagcataacc ggagaaatga gcgccaccag
    28321 caccacgccg ctaatcagcg gtagcagcgc cagcatggct tctctgatca gcagaataat
    28381 ttgcccgagg atcagattcg ggtctttgat aatactgtga tcaaaatgca gcccagcgga
    28441 gagcatgccc gacaatcgac gggccagcga cacaccgcca aaccagataa cactaacgcc
    28501 caccagcaaa atcagcagtg aggtcagttc acgggaacgc gggatttgcc cctcttcccg
    28561 cgctttttct agtcggtgag gtgtgggggc ttctgttttg tcgtcgctct cgtcagacac
    28621 gtcgccaatc ctggaaagag tattaagcgt aaatgatgcc agagcgcaaa gcgttcaatg
    28681 gtttgagtaa ggggcaaaac aggcgggatt tagggctttt gctgccacac atcaagcata
    28741 gtgtgcgttt gtcggatgcg gcgtcatcgc cttatcagac cgcctgatat gacgtggtca
    28801 cgccacatca ggcaatacaa atcaaaatcc aagactatcc aacaaatcgt ccacctgatc
    28861 ctgactggct accacaccgg ctttgctggt atcgacctga ggtccattaa gcaaactctg
    28921 gttttcacgt tttggacgcg actcctgttc cgggatgttt tccaacagca ccatcagcaa
    28981 ctggcgttcg atctcctgaa tgacatccat catccgctta atgacctgcc cggtgagatc
    29041 ctgaaaatcc tgcgccatca tgatttccag cagttgcgcg ttagtaaagc tggtatgcgc
    29101 gggtacatct gccagaaatt tccgcgtatc tgttaccagt tcacgggcgt cggcaaggtc
    29161 aatcggatcg gcaaaccagt catcccaacg ttgggttaac gcttttgctg atttctccat
    29221 ttgatcctga tgcggttgtg acgcctcaac actgttcagc gcccgctccg cagcctgggc
    29281 ggtcatctgc acaacatagt acaaacgatc gcgcgcatcg gggatggctt ccgccgcttc
    29341 ggcaatggcc tgatccagtc ccagttcccg caaactgtcg cgcagcatac gcgtcaggct
    29401 gccgatgcgc gcaatgatat cgccagctga atgctcgtca gcaggtttga ttgatggttg
    29461 catcatagtc gcatcctcac atgcccagtt tctcaaagat tttgttgagt ttttcctcca
    29521 gcgtcgcggc ggtaaatggc ttcaccacat agccactggc ccccgcttgc gccgcagcaa
    29581 tgatgttctc tttcttcgct tctgcagtca ccattaacac tggcaatgcc gacatcgcgc
    29641 catccgcacg aatcgttttc agcaattcca ggccatccat gttgggcatg ttccagtcgg
    29701 agataacaaa tccataaccg cctgcctgca acttattgag agcgtcgacg ccatcttccg
    29761 cttcctcaac attattgaat cccagctctt tcagcaggtt acgcactatg cgtcgcatgg
    29821 tggaaaagtc atccacaacc aaaaatttaa gttctttatc cgccatttca cactcctgat
    29881 ttaaatacgt atcgcctgtc cggcactaat ttttgccaac atttgctggc ttacctggtt
    29941 aagatcgacc acttcgcaga caccacccat attgatggcc tcgcgcggca tgccgaacac
    30001 cacgcaactt gcttcgtttt gcgcaagggt ccatgcccca gcctgacgca tcgccaacat
    30061 tcccgccgcg ccgtcgttgc ccataccggt caggatcacc ccaaccgcat tacgccccgc
    30121 ctgtttggcg acagaatgga acaacacatc taccgaaggc cgatgacggt taaccgccgg
    30181 gccatcgtga attttgattt ggtaatttgc gccactacgc gccagctcca tatgccgatc
    30241 gcccggcgca atataggcat gccccggcaa aacacgttcc ccgtcttcgg cttctttaac
    30301 cccgatctgg caaagcttat taagtctgtc ggcaaaagag cgggtgaaac cgggcggcat
    30361 atgctgggta attaacagtg ccgggctgga aagcggcaac ggttgcagta cgtgacgaat
    30421 tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt ttttcagaac tcaacaacgg
    30481 ccctgccttc agcgttgtcg gtgccgacaa tggcttatgt gctgcaaggc tcgcctttgc
    30541 tgctgtacgc accttttcag caatcatttc gctgtacgcc agcattcctt cgcgaatacc
    30601 cagttgcggt ttggtgacaa aatctattgc ccccagctcc agcgcgcgca gcgtgacttc
    30661 tgatcctttg ccggtcaggg aagaaaccat cacaacgggc attggacgca aacgcattaa
    30721 tttttcgagg aaatccagtc cgtccatccg cggcatttca acatccagcg tcagcacatc
    30781 gggattgaat ttcttaatca agtcacgcgc gaccagcgga tcaggcgcgg tcgccaccat
    30841 ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc tggcgcatca gtgccgaatc
    30901 atcgacagat aacaccctga ttttgctcat cgttaatcct tacttagcgc atacaccgtt
    30961 tgaccacgca gcgtgaagcg gcgctcgagg tggctaaagt tttcagagtg acccgcaaac
    31021 agcaatccgt cgggtttaag gagcggaaca aagcggcgca aaatctcctg ctgggtagtt
    31081 tgatcgaagt agatcatgac gttacgacag aagatcgcat caaacggccc cggcacggtg
    31141 tactgtttcg ccagtagatt cagcgaggca aaatcaacat agttcgccag ctcctgacgc
    31201 acgcgtacca gcccttcatg cggtcccgtc cctcgcatga aataccgttg cagttgctgc
    31261 ggcgtcaggg ttttcaactc ttcatggcga tagataccgc ttctggcttt ttccagcact
    31321 tcggtgtcga tatcactggc aaacactttc cagcgtccgg gcgcggtgcc caatgtgtca
    31381 gccagcgtca tcgcaatgct gtacggctct tcgccggtcg aagccgccgc gctccatacg
    31441 cgatactcgc cagaacggcg acgtgcgtga tccgcgagca gagggaaatg atgtgcctca
    31501 cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa acgcctgcca ctcaccgctg
    31561 tgctgattag attccagcaa gttcagataa tgaccgaaat ccgtcagtcc cagcgaacgc
    31621 aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat ggtcagccag aacgatcccg
    31681 gctcgttgat agatcaattg acttatccgc cgaaaatgcg cgtcggaaag cgccaggcgc
    31741 tcggtcatct gtaacaataa agacgtttgc ccacagggca gagatgaagt catagcgcct
    31801 tctcaatcac ttcaggatac cactggcgca atttgtaact gcgccgactc atgtcgtgct
    31861 acttcatgtt cttcaagggt aaataccgcc acacgcgacg aaagatggtc ggcctgattc
    31921 gccagttgtt ccgttgccac cgccgcttct tctaccagcg aggcgttctg ctgtgtcacc
    31981 tgatccatct ggctgacagc ctgcgcaacc tgctcaatcc cccgccgttg ttcttccgag
    32041 gccgaagcaa tttctcccat aatgtcgttc acgcgggtca ccgaactgac aatatcggtc
    32101 atggtcgcgg cggcgttatt caccagtttc gaaccctgct ggacacgatt gactgactct
    32161 tcgatcagcc ctttgatctc ttttgccgcc tgggcgcttc ggctggcaag attgcgcact
    32221 tctcctgcca ctaccgcaaa accacgcccc tgctctccgg cgcgagccgc ttctactgcc
    32281 gcattcaggg ccagaatatt ggtctggaaa gcaattccgt cgataacgct gataatgtcg
    32341 ccaatttttt gcgagctggt ggcgatctcc tgcatggtgt gagtcatggt actgacctga
    32401 acaccgcccg cctgcgccgt tgtcgcggca ttttttgcaa gttccgacgc ctgtcgtgcg
    32461 ttatcggcgt tttgccctac cgtggcggtt aattgctcca tactggcggc cgtttgtgcc
    32521 agcgatgccg cctgctgttc ggtacgactt gagagatcgt tattgcctgc gacaatctcc
    32581 gcgataccaa tgtgcatctc ctggcttccc ttacgcacat cacttaccgt cccacgcaaa
    32641 gcctgctgca tggtcttcag gctggcaaaa atggcggtga tctcattacg accatatacc
    32701 gcaatcggac gcgccagatt acccgcagca atgctgtcaa aatggctacc gataatggcc
    32761 agtggttgaa caatcatctt gcgcgtccac cacagcgcac tgctgatgta gatcgccgca
    32821 acaataatca tgctgataaa caccagcgcc gaaatctgat agttacgctg gctttgcgca
    32881 ctggcggctt ccagcacatg gatgatctcc agctgccagg cctcaaagtt aacgtcaaac
    32941 gcattctgtg atccctgcac cggcgcagtg aggaaatccg aaagttgatt actttcaagc
    33001 caggtcgcct gatgttcgag atcgttgtgc cagcgggcaa aacttttctc cgtctctttt
    33061 tgcaatgccc tgacgtgctc gttgcccgca gtcatcgcca taaaactttt aaacagcgtg
    33121 gtggattgcg tcagactggc acgcgccgtc gtcatcaacg ttttaatgtc atccgccgga
    33181 tagctaagcg ctgtcagagt gcctgctttg ttcagcgtgg tactggcctg taacattacc
    33241 gcccgagttt gcgctaatgc cgcacgttgc tgattgctct gctcgacctg attcagtcgt
    33301 tgcaaatcgt cgcgaaatgc ccaaaaagac atgccgttac tgccaatctg caagatcccg
    33361 cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa tacgattaaa catcaacgct
    33421 ccccatcagg cggcaatgac cgcgttagta aatactcgtc aaaatgtttc ccagtttgga
    33481 tcttgttcag caattcgcag tcgtggctgt gccggtggtt gctcactggc aggacgggat
    33541 ggtgtttgcg gtttattggt gagtgggctg gctgccagac gaaacgcgga aaccgcttgc
    33601 gttaaacgac tagcctgttc ttccagcgca gctgcagcgg cagctgattc ctgcaccagc
    33661 gatgcgttct gttgcgtgac gtgatccatt tccgaaaccg ccaatgcgac ttgatcaatg
    33721 ccacggctct gttcatccga tgccgatgca atctcgccca taatgtcagt cacgcgagtg
    33781 acagcatcga cgatattgtt cattgtttcc ccggcgcttt cgaccagcac cgaaccggta
    33841 tcaacgcgcg agacggagtc ttcaatgaga gctttgatct cttttgccgc ctgggcgcta
    33901 cgactggcaa gattacgcac ttcgcccgcc accacggcaa aaccacggcc ctgttcaccc
    33961 gctcgcgcgg cttcaacagc ggcattcagc gcgaggatat tagtctggaa ggcaataccg
    34021 tcgattacgc tgataatgtc ggcgattttc ttcgaactat cggcgatctc atgcatcgtt
    34081 ttcactacgc catccaccac tttgccgccg tgctgggcgg tgtcggaggc actttgcgcc
    34141 agttgcgagg cctggcgggc gttatcggcg ttttgcttca ctgtcgcggt gagctgctcc
    34201 atgctggcgg cagtttcttc cagcgcggat gcctgctgtt ccgtacggga ggaaagatcg
    34261 gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg catctgaacc ttcgcggaca
    34321 tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa cgctctgcgc caggtcgccc
    34381 atttcactgc gcccgtcaat ggtcagggta ttcgccaggt taccaccggc gatttcgcga
    34441 atgtgagcaa taatttttgc cagcggtgta agcaacatac ggcgaatgcc ataccacgcc
    34501 accagcagaa tcaataccac caccagcgcg ataaccgcca gttgccactg ggcaaatcgg
    34561 taatcatctg cgttgtcagt gacgatatcg cgatacagtt tttcactgct gagggcgtac
    34621 tgagcaaacg cttcgcccat tgcattttgc attccctggg ttggctgagc gaaataagct
    34681 ccagtattgc cataatcaag ataatcaatc agttcagtta acgctgtgta atagttttta
    34741 tatttttcat caatattacg actggtagcg accatttcag gtaacggtgc catgcttttg
    34801 aattttttat aatgagtcgc ggcctgggcc aatgttttcc tggcgctatc gagcaattca
    34861 actttggcat tactttgttg attggaggaa tccatcatca ttcgcaccgc tgaacgactc
    34921 aggttaatgc gcgtttgcag cattaaatcc caggttgacg tcagctcgcc ctgctgttct
    34981 cgtaattgat tggaaaccac aaagctcttc tggctatggt gaagggaaga aaaaaacaga
    35041 ctgccggaaa taagctgtaa cagtgcgaat acccccagca ccattaccaa cagcgtgact
    35101 acgcggatac ggttaatcat aaggcacctt cctgaaaaca agttgatctc gttatcggca
    35161 aggaaggggg aaactttatt gctgatgcca cccgccgcga aattgaaata aaacccgatg
    35221 cgcagatcat cgggttcatt tcaattgagg aaatcaggag aattacgcca cttctgacgc
    35281 cgcgctatct aacagcgcca tctcttcgct gttcagcagt ttttcgatgt tcaccagaat
    35341 caacatacgg tcgcccagtg cgcccagtcc agtgagatat tctgttgaaa gcgtcacggc
    35401 aaactctggt gccggacgaa tttgctccgc cgtcaatgaa agcacgtctg aaacgccgtc
    35461 aaccacgatg ccgaccaccc gctgtccgag attcaggacg ataactaccg tgttgtcgtt
    35521 atagtccaca tccacctggc tgaacttaat tcgtaagtca acaatcggca caataacgcc
    35581 gcgcagattc gtgacgcctt tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc
    35641 gtagccacgg atctcctgca ctttcaggat atcaataccg tactcttcat caccaagggt
    35701 aaataccaga aattcctggc ctgacggctc gctggccagc tttgttacat tcgtcatacc
    35761 ggtcatattg ttaccttttt actcattcag gcggcggtgt tcgccatacg ttgttcgcgg
    35821 tttatcgcct gcaaggcgga gacatcaaca atcagtgcca cgctgccgtc gccaagaatg
    35881 gtcgcagcag aaatgccggg gactttgcga tagttacttt caaggttttt aactacaacc
    35941 tgatgttgac caattaattg atccaccagc aaggcgtagc ggcgaccgcc actttgtaag
    36001 atcaccacaa ttccctgggt agcttcggtt ttcgcgcccg cgacgttgaa cactttccac
    36061 agttcgacga tgggcagata ttcaccccgc acttccagca cccgctcgcc gccggccagt
    36121 ggatgcagat cggcttcacg gggttgcagt gattccataa cagcattcag cggcagaatg
    36181 aatacttcat ccgcaacgcg tacggacatg ccgtcgagga tggccagcgt cagcggcagt
    36241 aaaatgcgga tcgtagtgcc agtaccctgc ttcgactgga tttcaacatg accgcccatc
    36301 tcctggatat tacgtttaac gacgtccatg ccgacgccgc gcccggagac gtcggtgacc
    36361 tgctctgccg tggagaagcc tggtgcaaat atcagcatcg cgacttcgtc gtcgctcatg
    36421 ttttcgctga cagtcaaacc ttgcgaggcc gcttttgcca gaattcgctc acggtttagc
    36481 cccgccccat cgtcggtcac ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga
    36541 attaaatttc cgacgctgtt tttacctgcg gcgagccgtt tttctggcag ttcaataccg
    36601 tgatcgaggc tattgcgtac caggtgggtc agcgggtcga taatgcgttc tatcaggctc
    36661 ttgtcgagct cggtggaact gcccaccagc gtcagttcta cctgcttgcc gagttttccc
    36721 gccagatcac gcaccagccg gggatagcga ctaaagacat attccatcgg catcatgcga
    36781 atcgacatca ctgattcctg caaatcacgg gcgttacgtt gtaactgccc catgctggta
    36841 atcaaatcac catgattaac cgggtccagt tcgctggaac gctgggcaag catggactgg
    36901 gtgataacca gctcgccgac gaggttaatt aattgatcaa ccttttctac cgctacacgg
    36961 atgctggtgg attcactgct acgcgtcgtt ttttcccgct ccacgcgacc ggttggcgct
    37021 tgttcggctg ccagtttaag cactggtggg gtggatattt ttggcgagac ttctactgtt
    37081 tcaaaggtaa tctgatcggc ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg
    37141 gcgatatcgc ccggtaatat tgccgagaga gaatccgccc ctttcaccac gtcagttaac
    37201 gttgtcagat gccccagctc ttcttccagc aggtcgactt cgctggcctt caggcgcgaa
    37261 aggataattc gtcgcggcaa ctgactgcga ctctgctcat cttgcggttc acttttggca
    37321 accacactta atcgggtcac tgcggatggc gtttcgcctt tcgcttctaa tgccagttga
    37381 cgcaaggcct ggcagatata atcgaagctg gcggcatccg gctcttgcga ctgtttataa
    37441 gcgtcgagct gttcttgcat gatgtccttc gtttccaaaa acagattgat aatgtcggtg
    37501 ttgagttgca tctcacctcg tctggcttca tcgagcaggt tttccatcag atgcgtggtt
    37561 tcctgcaaaa cgctgaagcc aaaagttcct gcccctcctt tgatcgagtg ggcagcccga
    37621 aagatggcat tcaattgttc ggcatctggc gcttccggct gcaaaaccag cagatgctgc
    37681 tccatatcag ccaacagttc gtccgcttca tcaaaaaatg tctgataaaa atcgcttata
    37741 tccatgctca cgctgtcacc tcggttcggc tgatggcatt gtgggaacac tgacctgtgg
    37801 tgcaacctca ggtttttcca gggcgcttac tggctcattc tggctttcgg cgttttcatg
    37861 caaaatggcc tgttcggctt gtttgttcag taccagcagg ctgatgcgac ggttgacggc
    37921 atcatcaggt ccgcgatcgc ttaagcgcat cgttgccgcc atgccgacca cacgtaacac
    37981 tttgccgcta tccaaccctc cgaccatcag ttcgcggcgg gatgcattgg cccgatcggc
    38041 agaaagctcc cagttgctgt aacctttctc accactggcg taggggaaat catcggtatg
    38101 acccgaaagg ctaatacggt tgggaatacc attcagaacg ggcgcgatgg cacgcagaat
    38161 gtcgcgcata tagggttcga cgtcggcact gccagttcta aacatcgggc gattctggct
    38221 atcgatgatc tgaatacgta gaccttcctg gaccagatcg attttgagat gtggacgtaa
    38281 cgcccgcagt ttcggatcgg actctatcaa ttggtcgaga tcgccacgca atttccgcaa
    38341 tcgactttgc tccatgcgtt ttttcaactc ttcgatgttc ggctgcttat tcacttcccc
    38401 ctggctttgg gtgtaatcat cgccaccgcc gggaattggg ctttcactat tagaaatgcg
    38461 atcgccgccc gtaaccgcag tcgccagtgg agtccggaag tactccgcaa tctgaatcaa
    38521 ctcttttggg ctggagatgg agatcagcca catcaccaga aaaaaggcca tcatcgcggt
    38581 cataaagtcg gcataagcaa tcttccacga tccatgtgct gccccgtggc ttttggcttt
    38641 gcgtcgtttg acgacaataa tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc
    38701 tgttgttgcg gatttttcac cgcacgcaca tgctcttcca gttcaataaa cgacggacgt
    38761 tcgctggaat agagcgtttt gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc
    38821 agattagaaa gcagtgtgac tttgacgcac tgcatcattt tgctggtttc ggcgcttttc
    38881 tgacgtaaaa cactcgctaa tggggaaata aatccgtaag ccaataaaat gccgaggaaa
    38941 gtccccacca tcgcatgtgc gataagcgcc cccagctcgg cggcaggacg atcggctgaa
    39001 cctaacgcgt gaacgacccc cattacagcc gcgacaatac caaacgccgg aagtgagtcc
    39061 ccgaccagcg ccagactgtt cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct
    39121 tcatccatca gggcttcgat ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga
    39181 taatcgacga taaaatcaag catgacgcta tccgcgatga tgcgtgggta gctggcgaag
    39241 atctcgctct cacggggatt ttcaatatca cgttccagcg aaaacatccc catctgccgc
    39301 gatttcgcca tcaaccgata aagcagagcc agcagatcca tatacattgc tttggtgtat
    39361 ttggagcgac gaaacagcaa cggcagcgct ttcagcgtgc ctttaatcgc ttttccatta
    39421 ttgccgacaa taaacgaccc aatccctgca ccggcaataa tcaccagttc agcgggttga
    39481 tagagtgctc caaggcttcc accggtcatc aaataaccgc cgaaaactgt accgagaaca
    39541 accaggtaac ctaataagat aagcacgaca tcatccttcc actgttgacc atgacaggat
    39601 gttcagtcgt caggcgttaa cgcgcgattg gggcaaaaaa aagcagcggt acgtcgttac
    39661 cgctgctgga atgttgcgcc acaccgtatc agttaaacag cctgtactct ctgttcatcc
    39721 agcagttgtg ggataatatc ggcaggattc tgggaaagtt tacgtctttt tactgcccgg
    39781 gatggcggtt gacataagct gcaggcaaag ctgccaacag gctggtgagc gtgggtaata
    39841 aaattgccgc cgcagcagtt gcagctggaa agttgcagta atccactttc aacaaaccgc
    39901 accaatgtcc aggcacgggt taatgccagc agcggtcctt cttctgcttg cgggcactgt
    39961 tcaaggtata aacggtaggc tttgatcacc gcatcgacgc cattacacaa accggttttc
    40021 agtaaaaact gccatgcatt acagaacatc gaagcatgaa cgttttgttc ccaagtcata
    40081 aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg ggcttccgcg cagttcttta
    40141 taaagtttta tcaggcgtcc gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga
    40201 gcgcccaggg tgatcaattc cattgccagc tgaatatccc gcgcttcctg aacaatgctt
    40261 ttttcactca tgatcaggcc cttttcttgc gcagcgcttc ttcaggctga ttaacatcat
    40321 tcagcaagcg tgttgagagc atgatgccgg tatgaatttg ctggagatcg tcaacgcggg
    40381 aatcttgcgt caacagagta atcgtctggt ggctgtcaaa acggaagtga caaaccagct
    40441 gattggtttc tgccagctta accatttgcg gaagagtcag tgccgctaat gttgtcgcca
    40501 tttcttcatt tatgccgaga cgaaacatag cggacgcttt gtcctgaaca atcagacgct
    40561 gtgcaagtag taaatatgac aagttgatgt cataaatgtg tttcagcaac tcggaggtat
    40621 gcattattcc cacccagaat aaccaacttt atttttatgc ggtttcaccg caccccgtga
    40681 tgtcgccggg aagccccggt aaaaaataat tagcattaga atagttgcga taagctgcaa
    40741 taagcagagc cacctttttg gtttaataag tccttacaaa tagaaggggg tctttacact
    40801 tatctaagat ttttcctaaa tcgacgcaac tgtactcgtc actacacgca catacaacgg
    40861 aggggggctg cgattttcaa taatgtgtga tgcagatcac acaaaacact caattactta
    40921 acataaatgt ataagtcata cttttgtttt gggtgtattt caatctgtta aaaagttttt
    40981 cgctacgcta gcaagcaaaa atgaaacagg aataatcgaa atgggatgtt gcgcacagtt
    41041 aaaataactc accgtaaata atcatctgct ataaataatc actttcatgc aataccagat
    41101 aagctatttt taaacagaca cttaccgcac aacaaactaa ttaaatacaa agaaaggcaa
    41161 caatcaactt aatgttaata aaatgttttg catatattaa ccacttgttt aatgcgggga
    41221 atcagtacat tattttctta taattacagt tgaaatcata tgatcaccga gagtgattga
    41281 ttaattgtct ttactaataa tcagactaat atttacctgt ttgactgagt tgggattgcg
    41341 tcgtttctcc attaggagta aagctttaat gtcacctgaa gttcacagaa taaggaacag
    41401 gttatgagct atagcaatat tcttgtcgct gttgcggtta caccggaaag tcagcaactg
    41461 ctggcaaaag cggtatctat cgccaggcca gtaaagggac acatcagttt aattactctc
    41521 gcttccgacc cggaaatgta caatcaatta gctgcgccga tgcttgaaga tttacgcagc
    41581 gtgatgcagg aagaaacgca aagctttctt gataagttaa ttcaggatgc ggggtatccc
    41641 gttgacaaga cgtttattgc ctacggtgag ttaagcgaac atattctgga agtttgtcgc
    41701 aaatatcatt tcgatttggt gatttgcggt aatcacaatc acagtttctt ttcgcgagca
    41761 tcctgctcgg cgaaaagagt gattgcctca agtgaggtcg atgtgctgtt agttccactt
    41821 acgggagatt aaccgctcct acgcaagctt aggaaaggta gcaactttat cgcgctgctg
    41881 gctttccgcg cttcgcggaa ctatctgctt taggtcgcta atgaagcact cctgccagtg
    41941 gttaatatcg tttttcacga taacgtccag catttctgca tgacgggaaa tacgttccgc
    42001 cagcgacata gtcaatgcac gatccagcgc agctgcaact tcgtcacgat cgtagggatt
    42061 aacaattaac gccgacgtta actcgtttgc cgctcccgca aattgcgaaa gaacaagaac
    42121 gcccgggttg gctgggtcct gagcggcaac atactctttt gctaccaggt tcatcccgtc
    42181 acgcagtggc gtcactaagc ccacgtcaga gtagcggaat attttcatca gtaatttacg
    42241 gtcaaaatgc tgattcaaat aataaagcgg cgtccagcct aattgcccgt atttaccatt
    42301 aattcgtccg gcttcatttt cgagctgatg acgaatatcc tgataggctt gcacatcacc
    42361 acgcgacgtt ggtgcaatct gggtataacg aattttacca tgatgctgcg gatatttttc
    42421 cagcaacgct tcataggcga gaaaacgctc tggcaaacct ttggaataat ccagccgttc
    42481 gacagaaaag atattttgta cgtttttcag ttccgcttta agttgcgcca gttttggcgg
    42541 cagtggcccg gcagcctgtt tggctatttc ttttggctca atgccaatcg ggtaaacttc
    42601 tgttcgaaac gctttgcccc aggctgtatg gctttttgcg ctacgtgtcg tgacgcgggt
    42661 caggttagaa aggcaatcca ggaacgccag acgatcgttt tctgtctgga aacccagcaa
    42721 atcataatca caaagctgtt caagcaaggt gtcatatgtc ggcagcgcgt tgaagatttc
    42781 cggtgtcggg aaaggaatat gcagaaagaa accaatgcga ttattcactc cccgtttgcg
    42841 taattcatgc gcaaatggca acaggtgata atcgtggatc cagataatgt catcgtcttg
    42901 caacagcggc agtaatttat ctgccagcaa cgcatttacg cgtagatagc cgtcccaggc
    42961 aggacgctga aattgcacca gatcgaggcg ataatgaaaa gcaggccaga gaacggcatt
    43021 ggagaattgg ttgtagtatt cgtcaaggtc ctgttcgctg aggttaaaag aggcccacgt
    43081 aatgttaccc tttttcacct tttttagcgg ctgatcctca ttccctgttt caccactcca
    43141 gccaaaccac agtccgcctg cggctttcag tgcccccagt atgccaacgg caaggccacc
    43201 ggcactggcg gcgtgctcat ctggtggtgc aatccggtta gatactacga ctaaacgact
    43261 catagtcatc actcctgtta ttttctcttt tttgttgtaa tgcggtggtt atcatttcaa
    43321 gccagctcca gacatccggt acgcccgcca gtcgccatga tgcctgagtt gcgcctgtgc
    43381 caatttttac tgacattccg cccagtcggt taacgactgc gaagccagat tcatcggtta
    43441 aatcatcgcc cagaaatacg ggcgttcgcc cgataaaggg agcttcctgc ataaaggcag
    43501 caatcgcctc acctttactg gtacctctcg gtttgatctc gacaacacac tttccctgct
    43561 gtaacgccat ttgtggccag atctgagtaa tacgttgcgc taatgtcatt aatgcgtctt
    43621 catgctgcgg agcctgacga taatgcagcg caaaagccat ccctttcgtc tccagctccg
    43681 caccgggata ctgggcgatg accgtatgca gttgcacgtt aatatcacgt gcaatcgcat
    43741 ccggcagatg aacgatatgc gttttaccat tgatgtcacg gcgctccgcc ccatgcacgc
    43801 ccgctaacgg gaagcgataa ggttttgcca gtgcgtccag ctccaccatt gagcgccctg
    43861 atatcaatgc caatgcacca tcacttgcgg ttgccagtag ctgaagtcct tgcagaatat
    43921 tgtcaggtac gacgacctga tcgggatgcg gtttgatttc cgccagcgtt ccatcgagat
    43981 caaaaaacca ggcatatttc gcggatagtt caggggtttc ggttaacggt tctgtcaccc
    44041 ggttctcctc cttctctttt cttattgtgt tgcgcagaac gcgctttaca gacatgtaag
    44101 tatagacagt gtgacggggg tcgccatttg caacgaagaa cagccaaccg ttgtggcggc
    44161 tggctgtggt gggaaaaaac gttaaattgt tgtggaaaaa agcatcagac agtgcgtttc
    44221 gctttttgct tgtaacggtc gaagatcacc gctgccagca ggattaagcc gcgaaccacg
    44281 tactgcgcga aaggagaaat attaagcagg ttcatggcgt tttccacggt gcctaaaatt
    44341 aagatacccg ccaccacata tgagattttt ccgatgccac ctttcagaga aacgccacct
    44401 aaaacgcagg cggagataac aataagctca taaccaatcg acgtcattgg ctgcccactg
    44461 gtcatacgtg aagccagaat aattccggct atcgcagata ccaggccaga gagaacaaag
    44521 ataataattt tggtgcgagc aaccggtaca cccgccagac gcgcagcctc ttcgttcccg
    44581 ccaatcgcca gggtattacg accaaaggta gttttattca gcagcaaacc aaagataatc
    44641 agacacgcga cggtgagcca gattggcgca ggcagaccga accagttggc gtaaccaagg
    44701 gcaaagaagc tttcatcttc gataccgacc gctttaccgt cggaaatgat gtacgccaga
    44761 cctcgaacaa tctgcatcgt tgccagtgtc gtgatcagag catttatttt cagtttggcg
    44821 ataacaaagc cattgaccag gccacagaga atgccaagca gcaaccccgc tgccacgcca
    44881 atccacaggc tttcagtcag attgataacc accgccgtgg tgacacccgc acaggcaatt
    44941 acggaggcga cagaaaggtc aaagtcaccg gaagcaaggc agaacaacat gccacacgcc
    45001 accatccccg acatggaaat tgccaggccc aaccctttca tattaataaa ggtggcaaag
    45061 ttcgggacaa aaatggcaca ggcgataaaa agcaccgcaa acaccaccag catgccgtac
    45121 tgatcccaga tacgcccgaa gctgaatgac gacttaggtg cgccagaacc cgatgtagaa
    45181 acagaagaca tcatactctc cttactcagg caactgcctg gctgacttta ggcatcgcaa
    45241 ggctcagtgc ctgacgctca tctgcctgct cgtgtaacaa ttcaccggcg atttcacctt
    45301 cgcgcatcac cacaatccgg tcggcaacgc cgaggacttc aggtaagtcg ctggaggcaa
    45361 acagcaccgc cacaccctgc gccgccagcg cataaatcac gttatatatt tcgtgcttag
    45421 caccaacatc aatgccgcgc gtaggttcat ccagcaaaat gaccttcatc tcttccgata
    45481 accagcggcc cagaatggct ttttgctgat ttccacctga gagattcata atcagttgct
    45541 cagcgcccgg cgttttgatg ttgagcgaac gaatgtggtg atcggcattg ttttcttccc
    45601 aaccgttgtt gattacacaa ccgccgagca catgtttacg tctggcactg atgttgatat
    45661 tgtcgcgaac ggagtgcaca ggaataatgc cttccgcttt gcgatcttcc gggcagagca
    45721 tcatgcctgc ggcaatggcg tggctcggtt tacgaatatc gatcggctgt tggtcgatat
    45781 aaacctgacc ggcggtgatt tgcgtcccgc caaacatgcc tttcattaat tcgctacgcc
    45841 ccgcccctac cagaccaaac agcccaacaa tttcaccact gcgaaccgcc agacttattg
    45901 gcgtacgcac gcctggtgct ttcacagcat caagacgcag gcgctcctcg ccataactac
    45961 gcggttgcca gccgtagata tcgccaatgt cgcgcccgac catcgcctgc accagcgcgt
    46021 cgtggtcaac ctgctgcata tcggtaaagg ttttgacata acgtccatct ttaaagacag
    46081 tgatggcatc gctgagggca aatatttctt ccatacggtg agaaacgtat aagatcaccc
    46141 gcccctcttt tcgcagttca cgaataacgc ggaaaagatt gtcgatttcg cgggcagaaa
    46201 gggagctggt tggctcatca aaggcgataa ttttagcgtt acgcgccagc gccttggcga
    46261 tttcaaccat ctgccactga ccaatggaga gatatttcag cggcgtgtcc gggtcaatat
    46321 ccataccaag atgtttaagt tgtaaacccg cctcataatt cagcaatgag cgattcacaa
    46381 tgccgccttt atgcggcagc tggccgagat agatgttttc cgcgacggtc atttccggca
    46441 cgagatgcag ttcctggtaa ataatcgcca cacccgcgtt aagtgctgcg gtcgtgtcgg
    46501 aaaaggacat ttcctgccca ttaatcacta cagaacccgt ggttggcgca tagttgccgc
    46561 tgaggatttt taagagagtt gattttcctg cgccattttc acccatcaac gcatgaacct
    46621 gaccggcata gcagtcaaaa ctaatatccg tcagcgcctt aacgccggga aacgttttac
    46681 cgatgccgcg aaatgagaga tacggggtag actgttgcat aacgtctccg tgaatcactg
    46741 gtcgttcaca ccccctctgc atcatgcaaa ggggaatttt tccggcaaat tacttaccgc
    46801 ctaaaccttt tttctccagt tcttctttaa agttgtcacg cgtgatcagc accacgtcgg
    46861 taacttcggt gaatttggtc ggttcaacac cttttgctac ccagttgtaa agcatttcgc
    46921 tggacttata accatgtacg tccgggcttg gcaacaggga accgtagaag ccggttgcct
    46981 gtgccttaga cagttcgctc accgcatcca caccgttaat accgataccg atgatatcgg
    47041 ccgctttaaa gccctgacct tccgtcgcgc gtacgccgcc cagcacggtg ctgtcgttca
    47101 taccgacgat cagccaatgt ttaacttccg gatgttgaac cagcattgag ttggcagcgt
    47161 caaatgcccc ggggatgtcg ttagatttgg taggtacctg ataaatttgt ttttccggga
    47221 atcctgcagc tttcagcgca tccatagaac ccgtagtacg gcgacgagcc gtatccagtt
    47281 cgttggaggt aatcgccatc accgcgcttt ctttgacatc ccagccacgt ttctgcatct
    47341 ctttatacag ttcctggccc tgacgttcgc caattttggt cgccgccatc atcaccagag
    47401 gaacggtatc cattggctta cctttggcgt taacaaactg gtcatccacg gcaatgactt
    47461 tcatatcgta gccacgcgct ttcgccacga tggcagagcc gagtttcggg tccggagtac
    47521 aaataacgaa accttttgcg ccactggcgg ccaggctatc gatcgcgttc aatgtttttt
    47581 cgccatccgg cacggcaatc ttaataacct caaaccctaa atccttcccg gctttatcgg
    47641 caaacttcca ttcggtctgg aaccacggct cttccggttg cttcaccagg aaaccgagct
    47701 tcaggttctc cgccatagcg gattgtgaca taacggctgc cagaccaatg gctgccaggg
    47761 ctttagtaaa tttgtgcatg gttctctcca gctttagtgt cgttttgtgc agggcaaaaa
    47821 cgaatgacat tcgttaaatt aatcggaaaa caaagcatta ccttttaact aaaagataag
    47881 tgactgtgtt gacatagttt tagcgagaaa ttaattctcc ataggagagc aatatcacat
    47941 cgcagaatta cagtgagaac gtgcataaat ttagcgggaa aagacataag gaaaagccaa
    48001 tttgtcagac aaattgttga atgcacagca gattaatcca taagattagc ctggaaatcc
    48061 ttgttgtctt tggtacccat gcgggatgtc ttctttttca ccagtcaata ggccgcatta
    48121 cctggcgttg agtttttgaa atggtgtaat aaccgcaact caaagatgtg gaaaatgcac
    48181 gtcattcatt tcgtcattaa ttatcactgt gctcattaat taacagaaca cgtataatga
    48241 gagccatctc gcaaaaatga aaaaacgttt tataaaataa tcacttcatc atgaattcaa
    48301 attcattgat taatatcaac aagatacaaa aa
//
LOCUS       DAZFBR010000023        47657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFBR010000023 DAZFBR010000000
VERSION     DAZFBR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.023"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(129..698)
                     /gene="espY2"
                     /locus_tag="ACHJZ2_001501"
     CDS             complement(129..698)
                     /gene="espY2"
                     /locus_tag="ACHJZ2_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFO4567645.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      940..1039
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1070..2053
                     /gene="thiB"
                     /locus_tag="ACHJZ2_001502"
     CDS             1070..2053
                     /gene="thiB"
                     /locus_tag="ACHJZ2_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO4567646.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            2029..3639
                     /gene="thiP"
                     /locus_tag="ACHJZ2_001503"
     CDS             2029..3639
                     /gene="thiP"
                     /locus_tag="ACHJZ2_001503"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO4567647.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            3623..4321
                     /gene="thiQ"
                     /locus_tag="ACHJZ2_001504"
     CDS             3623..4321
                     /gene="thiQ"
                     /locus_tag="ACHJZ2_001504"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO4567648.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(4408..5172)
                     /gene="yabI"
                     /locus_tag="ACHJZ2_001505"
     CDS             complement(4408..5172)
                     /gene="yabI"
                     /locus_tag="ACHJZ2_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4567649.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(5258..6136)
                     /gene="araC"
                     /locus_tag="ACHJZ2_001506"
     CDS             complement(5258..6136)
                     /gene="araC"
                     /locus_tag="ACHJZ2_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO4567650.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            6475..8175
                     /gene="araB"
                     /locus_tag="ACHJZ2_001507"
     CDS             6475..8175
                     /gene="araB"
                     /locus_tag="ACHJZ2_001507"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO4567651.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            8186..9688
                     /gene="araA"
                     /locus_tag="ACHJZ2_001508"
     CDS             8186..9688
                     /gene="araA"
                     /locus_tag="ACHJZ2_001508"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO4567652.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            9888..10583
                     /gene="araD"
                     /locus_tag="ACHJZ2_001509"
     CDS             9888..10583
                     /gene="araD"
                     /locus_tag="ACHJZ2_001509"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO4567653.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            10658..13009
                     /gene="polB"
                     /locus_tag="ACHJZ2_001510"
     CDS             10658..13009
                     /gene="polB"
                     /locus_tag="ACHJZ2_001510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO4567654.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            13173..16079
                     /gene="rapA"
                     /locus_tag="ACHJZ2_001511"
     CDS             13173..16079
                     /gene="rapA"
                     /locus_tag="ACHJZ2_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO4567655.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            16091..16750
                     /gene="rluA"
                     /locus_tag="ACHJZ2_001512"
     CDS             16091..16750
                     /gene="rluA"
                     /locus_tag="ACHJZ2_001512"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO4567656.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            16963..17141
                     /locus_tag="ACHJZ2_001513"
                     /pseudo
     CDS             16963..17141
                     /locus_tag="ACHJZ2_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(17168..17920)
                     /gene="espY1"
                     /locus_tag="ACHJZ2_001514"
     CDS             complement(17168..17920)
                     /gene="espY1"
                     /locus_tag="ACHJZ2_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFO4567657.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNANLDGATLDGATLDGATVDGATHLYDEVI
                     IINKITPKKIDTEEVATKQSTAEQITDNAIIE"
     gene            complement(18215..19030)
                     /gene="djlA"
                     /locus_tag="ACHJZ2_001515"
     CDS             complement(18215..19030)
                     /gene="djlA"
                     /locus_tag="ACHJZ2_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO4567658.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            19285..21639
                     /gene="lptD"
                     /locus_tag="ACHJZ2_001516"
     CDS             19285..21639
                     /gene="lptD"
                     /locus_tag="ACHJZ2_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO4567659.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            21692..22978
                     /gene="surA"
                     /locus_tag="ACHJZ2_001517"
     CDS             21692..22978
                     /gene="surA"
                     /locus_tag="ACHJZ2_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO4567660.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            22978..23967
                     /gene="pdxA"
                     /locus_tag="ACHJZ2_001518"
     CDS             22978..23967
                     /gene="pdxA"
                     /locus_tag="ACHJZ2_001518"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO4567661.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            23964..24785
                     /gene="rsmA"
                     /locus_tag="ACHJZ2_001519"
     CDS             23964..24785
                     /gene="rsmA"
                     /locus_tag="ACHJZ2_001519"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO4567662.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            24788..25165
                     /gene="apaG"
                     /locus_tag="ACHJZ2_001520"
     CDS             24788..25165
                     /gene="apaG"
                     /locus_tag="ACHJZ2_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO4567663.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25172..26020
                     /gene="apaH"
                     /locus_tag="ACHJZ2_001521"
     CDS             25172..26020
                     /gene="apaH"
                     /locus_tag="ACHJZ2_001521"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO4567664.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(26017..26331)
                     /locus_tag="ACHJZ2_001522"
     CDS             complement(26017..26331)
                     /locus_tag="ACHJZ2_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO4567665.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(26334..26567)
                     /locus_tag="ACHJZ2_001523"
     CDS             complement(26334..26567)
                     /locus_tag="ACHJZ2_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO4567666.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(26653..27132)
                     /gene="folA"
                     /locus_tag="ACHJZ2_001524"
     CDS             complement(26653..27132)
                     /gene="folA"
                     /locus_tag="ACHJZ2_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO4567667.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27324..29186)
                     /gene="kefC"
                     /locus_tag="ACHJZ2_001525"
     CDS             complement(27324..29186)
                     /gene="kefC"
                     /locus_tag="ACHJZ2_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO4567668.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29179..29709)
                     /gene="kefF"
                     /locus_tag="ACHJZ2_001526"
     CDS             complement(29179..29709)
                     /gene="kefF"
                     /locus_tag="ACHJZ2_001526"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO4567669.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(29817..31148)
                     /gene="yaaU"
                     /locus_tag="ACHJZ2_001527"
     CDS             complement(29817..31148)
                     /gene="yaaU"
                     /locus_tag="ACHJZ2_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4567670.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMALAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(31206..31493)
                     /gene="fixX"
                     /locus_tag="ACHJZ2_001528"
     CDS             complement(31206..31493)
                     /gene="fixX"
                     /locus_tag="ACHJZ2_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO4567671.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31490..32776)
                     /gene="fixC"
                     /locus_tag="ACHJZ2_001529"
     CDS             complement(31490..32776)
                     /gene="fixC"
                     /locus_tag="ACHJZ2_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4567672.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(32827..33768)
                     /gene="fixB"
                     /locus_tag="ACHJZ2_001530"
     CDS             complement(32827..33768)
                     /gene="fixB"
                     /locus_tag="ACHJZ2_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO4567673.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(33783..34553)
                     /gene="fixA"
                     /locus_tag="ACHJZ2_001531"
     CDS             complement(33783..34553)
                     /gene="fixA"
                     /locus_tag="ACHJZ2_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO4567674.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35027..36541
                     /gene="caiT"
                     /locus_tag="ACHJZ2_001532"
     CDS             35027..36541
                     /gene="caiT"
                     /locus_tag="ACHJZ2_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO4567675.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36572..37714
                     /gene="caiA"
                     /locus_tag="ACHJZ2_001533"
     CDS             36572..37714
                     /gene="caiA"
                     /locus_tag="ACHJZ2_001533"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO4567676.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            37843..39060
                     /gene="caiB"
                     /locus_tag="ACHJZ2_001534"
     CDS             37843..39060
                     /gene="caiB"
                     /locus_tag="ACHJZ2_001534"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO4567677.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            39134..40687
                     /gene="caiC"
                     /locus_tag="ACHJZ2_001535"
     CDS             39134..40687
                     /gene="caiC"
                     /locus_tag="ACHJZ2_001535"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO4567678.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            40796..41581
                     /gene="caiD"
                     /locus_tag="ACHJZ2_001536"
     CDS             40796..41581
                     /gene="caiD"
                     /locus_tag="ACHJZ2_001536"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO4567679.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41587..42177
                     /gene="caiE"
                     /locus_tag="ACHJZ2_001537"
     CDS             41587..42177
                     /gene="caiE"
                     /locus_tag="ACHJZ2_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO4567680.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42296..42691)
                     /gene="caiF"
                     /locus_tag="ACHJZ2_001538"
     CDS             complement(42296..42691)
                     /gene="caiF"
                     /locus_tag="ACHJZ2_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO4567681.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(42952..46173)
                     /gene="carB"
                     /locus_tag="ACHJZ2_001539"
     CDS             complement(42952..46173)
                     /gene="carB"
                     /locus_tag="ACHJZ2_001539"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO4567682.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(46191..47339)
                     /gene="carA"
                     /locus_tag="ACHJZ2_001540"
     CDS             complement(46191..47339)
                     /gene="carA"
                     /locus_tag="ACHJZ2_001540"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO4567683.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        11341 a        12876 c        12384 g        11056 t
ORIGIN      
        1 gcgtcgcaaa taagattaaa gaaggggcat tccagcccct ctttcatatt ctgagttaat
       61 caataacggc gcagtctagc cttgttctgg ccggaaaggt cagctttttc aggttaacct
      121 cgcgcaggtc agtcatacca acggctattg ttcgtttcag cattgctcag gtcaacctcg
      181 agacagtcaa taccacaaat gacaaccccg ttaaagtaag cattactcag atcaatcccc
      241 ccggtcctgg tgctcttttt cagctgcaac tccagagtaa atatcgcctc atcgccaata
      301 aaaaaactga cgcataacgt accttctggc gagagtgagg ccttcgcggc catattttcc
      361 ggatcttttg cgtattcttt gagttcatta aattttatca gccagtgagt taacttatca
      421 cgcgccttcc cgtcgacagc cccggtaaag tattttgagt agtcatcact attctggaaa
      481 taaaatgcat ccttcagaat ttgccttgcc gcagtctggg aatgtttatc tgactggaag
      541 agattgcgta acgtctccca tagctcttca aagatattca tattctcggt ctttttgacc
      601 aagttagcca tcctgataat ctgagaatta gtaaggcctg gatctttagt ggtattacta
      661 gcagggatac taatctgttc tgggtttctt actttcatgc tgatagctcc actctgaata
      721 aaaaactcag aataagcgtc ctgaataaga atactgtcaa aaatcgctca ttcacgcaat
      781 aaacggttac gttacctatt ttgactgtag cgaagggatt ctttcggatc aacgctctcc
      841 agctatgcaa atacgctctt gatagctgaa cgaatattgg cttttttaat acgccttctc
      901 tatggattgc tggttgataa caaaatcact acactaacgc cgttctcaac ggggtgccac
      961 gcggacgcgt gcgctgagaa aatacccgtc gaacctgatc cggataacgc cggcgaaggg
     1021 atttgaggct ccttctcaag tcctttgcca ctcttttttg aggtgcaaag tgttaaaaaa
     1081 atgtcttccc ctgctgttgc tgtgcacagc gcccgttttc gctaaacccg ttctgactgt
     1141 ttatacctac gattccttcg ccgccgactg ggggcctggt ccggtggtta aaaaagcctt
     1201 tgaagccgac tgtaattgcg aactgaaact ggtggcgctg gaagatggcg tttcgcttct
     1261 caaccgtcta cggatggaag gcaaaaacag taaagccgat gtggtgctgg ggctggataa
     1321 caatctgtta gacgccgcca gtaaaaccgg actgtttgcc aaaagcggtg tggcagcgga
     1381 tgccgttaac gttcccggcg gctggaataa tgacattttc gtaccgtttg attatggcta
     1441 cttcgccttc gtttatgaca agaacaaact gaaaaacccg ccacaaagcc tgaaagaact
     1501 ggttgagagc gatcaaaact ggcgggtgat ttatcaggat ccgcgcacca gtacaccggg
     1561 gctgggtctg ttgctatgga tgcaaaaagt ctatggcgat gacgccccac aagcctggca
     1621 gaaactggcg aagaaaacgg tcacggtcac caaaggctgg agcgaagcct acggcctgtt
     1681 tttaaaaggt gaaagcgatc tggtactgag ttacaccacc tctccggctt atcacattct
     1741 cgaagagaag aaagataact acgccgccgc gaacttcagc gaaggtcact atctgcaagt
     1801 ggaagtcgcc gctcgcaccg ctgccagcaa gcagccggag ctggcgcaaa aattcctcca
     1861 gtttatggtt tctccggctt tccagaatgc gatcccaacc ggcaactgga tgtatccggt
     1921 ggcaaacgtc acgctgcctg ccggttttga aaaattgacc aaacccgcaa ccacgctgga
     1981 gttcacgcca gccgaagtgg cggcacaacg tcaggcatgg attagcgaat ggcaacgcgc
     2041 cgtcagccgt taattcccgg ctggttaatt ccaggtgtaa gcgccgccac gctggtggta
     2101 gcggttgcgc tggcggcgtt tctcgccctg tggtggaacg cgccgcaggg tgactggtcg
     2161 gcagtctggc aggacagcta cctgtggcat gtggtgcgct tctccttctg gcaggcgttt
     2221 ctctcggcgc tactctctgt cgtacccgcg atattcctcg cccgcgcgct ctatcgcagg
     2281 cgctttccgg gtcggctggc gctgttgcgt ctgtgcgcaa tgaccttgat cctcccggtg
     2341 ctggttgctg ttttcggcat tcttagcgtc tatggtcgcc agggctggct ggcatcgctc
     2401 tgccaatcgc tcggtctgga gtggaccttt tcgccctacg gcctgcaagg cattttgctg
     2461 gcccatgtgt tttttaatct gccgatggcg agccgcttat tactccaggc actggaaaac
     2521 attcccggcg aacaacgtca gcttgccgcc cagcttggga tgcgcggctg gcattttttc
     2581 cgcttcgtcg aatggccgtg gttacggcga caaatcccgc cggttaccgc gcttatcttt
     2641 atgctctgtt tcgccagctt cgccaccgtg ttatcgctgg gcggcggtcc gcaggcaacc
     2701 actatcgagc tggcaatcta tcaggcgctg agttacgact acgatcctgc ccgcgcggcg
     2761 atgctggcgc tgatccagat ggtgtgttgc cttgggctgg tgctgttgag tcagcgactg
     2821 agtaaggcca ttgcgcccgg caccacgctg ctgcaaggct ggcgcgaccc ggacgatcgt
     2881 ctgcatagcc gcatttgcga cacgatgtta attgtgctgg cgctgctgct gttgctgcca
     2941 ccgttgctgg cggtgatcgt cgatggggta aatcgccagt tgccggaagt gctggcacaa
     3001 ccggtgctgt ggcaggcgct gtggacctcg ttgcgtattg cgctggcggc aggtgtattg
     3061 tgcgtagtgc tgaccatgat gctgctatgg agcagtcgcg aactgcgggc gcggcaaaaa
     3121 atgctggcgg gccaggcgct ggagatgagc ggcatgttga tcctcgctat gccggggatt
     3181 gtgctggcaa cgggcttctt tttactgctc aacaacacta tcggcctgcc acaatctgct
     3241 gacggcattg tgattttcac caatgcgtta atggcgatcc cctacgccct gaaagtgctg
     3301 gaaaacccga tgcgcgatat caccgcccgc tacagcatgt tatgtcagtc gctggggatt
     3361 gaaggctggt cacgcttaaa agtagtggag ctgcgcgccc tgaaacgccc gctggcgcag
     3421 gcgctggcct ttgcctgcgt gctgtcgatt ggtgattttg gcgtggtggc gttgttcggt
     3481 aacgatgatt tccgcaccct gccgttttat ctctaccagc aaattggctc ctatcgcagc
     3541 caggacggcg cggtcaccgc gttaattctg ctgctgctct gttttctgct gtttagcgtg
     3601 attgaaaaaa taccggggcg aaatgttaaa actgactgat atcacctggc tttatcacca
     3661 tttgccgatg cgttttagct taacggtaga acgcggcgag caggtggcga tcctcgggcc
     3721 aagcggcgcg ggtaaaagta ccctgctgaa cttgatcgcc ggttttctga caccagccag
     3781 cggtgcgctg actatcgacg gcgttgatca caccacgacg ccgccgtcac gccgtccggt
     3841 gtcgatgctg tttcaggaga acaacctgtt cagccacctg acggtcgcgc agaacatcgg
     3901 gctggggctt aatccaggat tgaagctgaa cgcggtacag caggagaaaa tgcacgcgat
     3961 tgcccgtcag atggggattg ataatttaat ggcgcggtta ccgggcgagc tttccggcgg
     4021 tcagcgacag cgcgtggcgt tagcgcgttg tctggtacgc gaacagccga ttttattgct
     4081 cgatgaaccg ttctctgcgc tcgatccggc gctacgtcag gagatgttga cgctggtgag
     4141 cactagctgc cagcagcaaa aaatgacgct attgatggtg tcgcacagtg tggaagatgc
     4201 ggcgcggatt gccacgcgct cggtagtagt cgccgacggg cgaatcgcct ggcagggtat
     4261 gaccaatgag ttgttgagcg gtaaggcgag tgcttcggcg ctgttgggaa ttacaggtta
     4321 atatgcactg tcggatgcgg cgtgaacgcc ttatccgacc tacgggtgcg ttttgtccgc
     4381 atcaagcgcc ccacacaaga ctatgcccta aactccaacc actttacgca aaatatcgat
     4441 atacaccggc attaacggat ggcgaactaa caccaccagc gccaccacgc cgatggcaga
     4501 aatcaacggc gtcagccaca acaaacgacc gcgggacaaa taatgactca aacgatcggt
     4561 tgctttgccg ctgcgccata accgccagca cagccagcca ccaacccaca aaaataccgc
     4621 cgtcgccagc agcaaccatt taaactcacc gctctgcatt ccggctggaa tatcgatcgc
     4681 cgcacccgcc agaatccccg gcaggaagta aaacggtggc cacagcaggc agccgataat
     4741 attcggcgtg ataaattttg ccaccgggag atccagcatt cccgccacca ttggcaccag
     4801 cggacgcgtc gggccaacaa aacgaccgac cagaatggtg aacatgctgt gttgatgcaa
     4861 cgcatgttca gttttatcga gtaatgcttt gttcttcttc agaaatgacc aacgatgcaa
     4921 tggcttttta aaacgccagc ccagccagaa ggagatccag tcgcccagca gacaaccgac
     4981 gatccctgcc agccaggcgt gccagaaact taactcgccg ctgccaatca gcgctcccag
     5041 ccccgccatc agcaccgtac cgggtagaat caaaccgacc agcgccagcg actccagaaa
     5101 ggccaccaac accaccgcca tcaatgaata cacggtggat tgggtaataa agtgttccag
     5161 caatgcttgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct
     5221 actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat
     5281 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
     5341 taaatactcg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
     5401 taggcatccg ggtagtgctc aaaagcagct tcgcctggct gatgcgttgg tcctcgcgcc
     5461 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
     5521 ggcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
     5581 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg
     5641 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
     5701 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt
     5761 gcgcttcatc cgggcgaaag aaacccgtat tggcaaatat tgacggccag ttaagccatt
     5821 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
     5881 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
     5941 agacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
     6001 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataacca ttggcctcaa
     6061 tcggcgttaa acccgccacc agatgggcgt taaacgagta tcccggcagc aggggatcat
     6121 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat
     6181 attgcatcag atattgccgt cactgcgtct tttactggct cttctcgcta acccaaccgg
     6241 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc
     6301 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgataatttg cacggcgtca
     6361 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt
     6421 tatcgcaact ctctactgtt tctccatacc cgtttttttg gatggagtga aacgatggcg
     6481 attgcaattg gcctcgattt tggcagtgat tctgtgcgag ctttggcggt ggactgcacc
     6541 accggtgaag aaatcgccac cagcgtagag tggtatcccc gttggcaaaa agggcaattt
     6601 tgtgatgccc cgaataacca gttccgtcat catccgcgtg actacattga gtcaatggaa
     6661 gcggcactga aaaccgtgct tgcagaactt agcgtcgaac agcgcgcagc tgtggtcggg
     6721 attggcgttg acagtaccgg ctcgacgccc gcaccgattg atgccgacgg taacgtgctg
     6781 gcgctgcgcc cggagtttgc cgaaaacccg aacgcgatgt tcgtattgtg gaaagaccac
     6841 actgcggttg aagaagcgga agagattacc cgtttgtgcc acgcgccggg caatgttgac
     6901 tactcccgct atattggcgg tatttattcc agcgaatggt tctgggcaaa aatcctgcat
     6961 gtgactcgcc aggacagcgc cgtggcgcaa tctgccgcat cgtggattga gctgtgcgac
     7021 tgggtgccag ctctgctttc cggtaccacg cgcccgcagg atattcgtcg cggacgttgc
     7081 agcgccgggc ataagtcgct atggcacgaa agctggggcg gcctgccgcc agccagtttc
     7141 tttgatgagc tggatccgat cctcaatcgc catttgcctt ccccgctgtt cactgatacc
     7201 tggactgccg atattccggt gggcacctta tgcccggaat gggcgcagcg tctcggcctg
     7261 cctgaaagcg tggtgatttc cggcggcgcg tttgactgcc atatgggcgc agttggtgca
     7321 ggcgcacagc ctaacgcact ggtaaaagtt atcggtactt ccacctgcga cattctgatt
     7381 gccgacaaac agagcgttgg cgagcgggca gtgaaaggta tttgcggtca ggttgatggc
     7441 agcgtggtgc ctggatttat cggtctggaa gcaggccaat cggcgtttgg tgatatctac
     7501 gcctggttcg gtcgcgtact cggctggccg ctggaacagc ttgccgccca gcatccggaa
     7561 ctaaaagagc aaatcgacgc cagccagaaa caactgcttc cggcgctgac cgaagcatgg
     7621 gccaaaaatc cgtctctgga tcacctgccg gtggtgctcg actggtttaa cggccgccgc
     7681 acaccgaacg ctaaccaacg cctgaaaggg gtgattaccg atcttaacct cgctaccgac
     7741 gctccgctgc tgttcggcgg tttgattgct gccaccgcct ttggcgcacg cgcaatcatg
     7801 gagtgcttta ccgatcaggg gatcgccgtc aataacgtga tggcgctggg cggcatcgcg
     7861 cggaaaaacc aggtcattat gcaggcctgc tgcgacgtgc tgaatcgccc gctgcaaatt
     7921 gttgcctctg accaatgctg tgcgctcggt gcggcgattt ttgctgccgt cgccgcgaaa
     7981 gtgcacgcag acatcccatc agcccagcaa aaaatggcca gtgcggtaga gaaaaccctg
     8041 caaccgcgca gcgaacaggc acaacgcttt gaacagcttt atcgccgcta tcagcaatgg
     8101 gcgatgagcg ccgaacaaca ctatcttcca acttccgccc cggcacaggc tgcccaggcc
     8161 gttccgactc tataaggaca cgataatgac gatttttgat aattatgaag tgtggtttgt
     8221 aattggcagc cagcatctgt atggcccgga aaccctgcgt caggtcaccc aacatgccga
     8281 gcacgttgtg aaagcgctga atacggaagc gaaactgccc tgcaaactgg tactgaaacc
     8341 gctgggcacc acgccggatg aaatcaccgc tatttgccgt gatgctaatt acgacgatcg
     8401 ttgcgctggt ctggtggtgt ggctacacac cttctccccg gccaaaatgt ggatcaacgg
     8461 cctgaccatg ctcaacaaac cgttgctgca attccacacc cagttcaacg cggcgctgcc
     8521 gtgggacagc atcgatatgg actttatgaa cctgaaccag accgcgcacg gcggtcgcga
     8581 gttcggcttc attggcgcgc gtatgcgtca gcaacatgcc gtggttaccg gtcactggca
     8641 ggataaacaa gcccatgagc gtatcggctc ctggatgcgt caggcggtat ctaaacagga
     8701 tacccgtcat ctgaaagtct gccgatttgg cgataacatg cgtgaagtgg cggtcaccga
     8761 tggcgataaa gttgccgcac agatcaagtt cggtttctcc gtcaatacct gggcggttgg
     8821 cgatctggtg caggtggtga actccatcag cgacggcgat gttaacgcgc tggtcgatga
     8881 gtacgaaagc tgctacacca tgacgcctgc cacacaaatc cacggcgaaa aacgacagaa
     8941 cgtgctggaa gcggcgcgta ttgagctggg gatgaaacgt ttcctggaac aaggtggctt
     9001 ccacgcgttc accaccacct ttgaagattt gcacggtctg aaacagcttc ctggtctggc
     9061 cgtacagcgt ctgatgcagc agggttacgg ctttgcgggc gaaggcgact ggaaaactgc
     9121 cgccctgctt cgcatcatga aggtgatgtc aaccggtctg cagggcggca cctcctttat
     9181 ggaggactac acctatcact tcgagaaagg caatgacctg gtgctcggct cccatatgct
     9241 ggaagtctgc ccgtcgatcg ccgcagaaga gaaaccgatc ctcgacgttc agcatctcgg
     9301 tattggtggt aaggacgatc ctgcccgcct gatcttcaat acccaaaccg gtccagcgat
     9361 tgtcgccagc ttgattgatc tcggcgatcg ttaccgtcta ctggttaact gcatcgacac
     9421 ggtgaaaaca ccgcactccc tgccgaaact gccggtggcg aatgcgctgt ggaaagcgca
     9481 accggatctg ccaactgctt ccgaagcgtg gatcctcgct ggtggcgcgc accataccgt
     9541 cttcagccat gcgctgaacc tcaacgatat gcgccagttc gccgagatgc acgacattga
     9601 aatcacggtg attgataacg acacccgcct gccagcgttt aaagacgcgc tgcgctggaa
     9661 cgaagtgtat tacgggtttc gccgctaagt agccgcatcc ggtatgtaac gcctgatgcg
     9721 acgctggcgc gtcttatcag gcctacacgc tgcaattttg taggccggat aagcaacgcg
     9781 catccggcat tcagcgcctg atgcgacgct gacgcgtctt atctggccta cacgctgcga
     9841 ttttgtaggc cggataagca acgcgcatcc ggcacgaagg agtcaacatg ttagaagatc
     9901 tcaaacgtca ggtattagaa gccaacctgg cgctgccaaa acacaacctg gtcacgctca
     9961 catggggcaa cgtcagcgcc gttgatcgcg agcgcggcgt ctttgtgatc aaaccttccg
    10021 gcgtcgatta cagcatcatg accgctgacg atatggtcgt ggtcagcatc gaaaccggtg
    10081 aagtggttga aggtgcgaaa aagccctcct ccgatacgcc aactcaccga ctactctatc
    10141 aggcattccc gtccattggc ggcattgtgc acacgcactc gcgccacgcc accatctggg
    10201 cgcaggcggg tcagtcgatt ccagcaaccg gcaccaccca cgccgactat ttctacggca
    10261 ccattccctg cacccgcaaa atgaccgacg cagaaatcaa cggcgaatat gagtgggaaa
    10321 ccggtaacgt catcgtagaa accttcgaaa aacagggtat cgatgcagcg caaatgcccg
    10381 gcgttctggt ccattctcac ggcccatttg catggggcaa aaatgccgaa gatgcggtgc
    10441 ataacgccat cgtgctggaa gaggtcgctt atatggggat attctgccgt cagttagcgc
    10501 cgcagttacc ggatatgcag caaacgctgc tggataaaca ctatctgcgt aagcatggcg
    10561 cgaaggcata ttacgggcag taatgactgt ataaaaccac agccaatcaa atgaaaccag
    10621 gctataatca agcctggttt tttgatggat tttcagcgtg gcgcaggcag gttttatctt
    10681 aacccgacac tggcgggata ccccgcaagg aacagaggtc tccttctggc tggcgacgga
    10741 caacgggccg ttgcaggtta cgcttgcacc gcaagagtcc gtggcgttta ttcccgccga
    10801 tcaggttccc cgcgctcagc atattttgcg gggtgaacaa ggctttcgcc tgacgccgct
    10861 ggcgttaaag gattttcacc gccagccggt gtatggcctt tactgtcgcg cccatcgcca
    10921 gttgatgaat tacgaaaagc gcctgcgtga aggtggcgtt accgtctacg aggccgatgt
    10981 gcgcccgcca gaacgctatc tgatggagcg gtttatcacc tcaccggtgt gggtcgaggg
    11041 tgatatgcac aatggcgcta tcgttaatgc ccgtctgaaa ccgcatcccg actatcgtcc
    11101 gccgctcaag tgggtttcta ttgatattga aaccacccgc cacggagagc tgtactgcat
    11161 cggcctggag ggctgcgggc agcgcatcgt ttatatgctg ggaccagaga atggcgacgc
    11221 ctccgcgctc gattttgaac tggagtacgt cgccagccgc ccgcagctac tggaaaaact
    11281 caacgcctgg tttgcaaact acgatcctga tgtgatcatc ggttggaacg tggtgcagtt
    11341 tgatctgcga atgctgcaaa aacatgccga gcgttatcgt attccgctgc ggctggggcg
    11401 cgataacagt gagctggagt ggcgcgagca cggctttaaa aacggcgtct tttttgccca
    11461 ggctaaaggt cggctaatta tcgacggtat cgaggcgctg aaatccgcgt tctggaattt
    11521 ctcttcattc tcgctggaaa ctgtcgctca ggagctatta ggcgaaggaa aatctatcga
    11581 taacccgtgg gatcgaatgg acgaaattga ccgccgtttc gccgaagata aacctgcgct
    11641 ggcaacttat aacctgaaag attgcgagct ggtgacgcag atcttccata aaactgaaat
    11701 catgccgttt ttgctcgaac gagcaacggt gaacggcctg ccggtggatc gacacggcgg
    11761 gtcggtggca gcgtttggtc atctctattt tccccgtatg catcgcgctg gttatgtcgc
    11821 gcctaatctc ggcgaagtgc cgccgcacgc cagccctggc ggctacgtga tggattcgcg
    11881 acctgggctt tatgattcgg tgctggtgct ggactacaaa agcctgtacc cgtcgatcat
    11941 ccgcaccttt ctgattgatc ccgtcgggct ggtggaaggc atggcgcagc ctgatccaga
    12001 gcacagcacc gaaggctttc tcgatgcctg gttctcgcga gaaaaacatt gcctgccgga
    12061 gattgtgact aacatctggc acggacgcga tgaagccaaa cgccagggta acaaaccgct
    12121 gtcgcaggcg ctgaaaatca tcatgaatgc cttttatggc gtgctcggca ccaccgcctg
    12181 ccgcttcttc gatccgcggc tggtatcgtc gatcaccatg cgtggtcatc agatcatgcg
    12241 gcaaaccaaa gcgttgattg aagcgcaggg ctacgacgtg atctacggcg ataccgactc
    12301 aacgtttgtc tggctgaaag gcgcacattc ggaagaagaa gcgacgaaaa tcggtcgtgc
    12361 actggtgcag cacgttaacg tctggtgggc agaaacgctg caaaaacaac agctgaccag
    12421 cgcactggaa ctggagtatg aaacccattt ctgccgtttt ctgatgccaa ccattcgcgg
    12481 agccgatacc ggcagtaaaa agcgttatgc cgggctgatt caggagggcg acaaacagcg
    12541 gatggtgttt aaagggctgg aaaccgtgcg tacggactgg acgccgctgg cccagcagtt
    12601 tcagcaggag ctttacctgc gtatcttccg caacgagcca tatcaggagt acttccgcga
    12661 aaccatcgac aaactgatgg cgggtgaact ggatgcgcgg ctggtttacc gtaaacgcct
    12721 tcgccgtccg ctgagcgaat atcagcgtaa tgttccgcct catgtacgcg ccgctcgtct
    12781 tgccgatgaa gaaaaccaaa agcgcggtcg ccccttgcaa tatcagaacc gcggcaccat
    12841 taagtacgta tggaccacca acggcccgga gccgctggac taccaacgtt caccactgga
    12901 ttacgaacac tatctgaccc gccagctaca acccgtggcg gagggaatac tcccttttat
    12961 tgaggataat tttgctacac ttatgaccgg gcaacttggg ctattttgag caaaaaaaga
    13021 gttcgccaga taccattttg atgcgtgacg aatgctttgc catccagtac catagcgccc
    13081 tttccattcc tggacctgaa taacaccact acctcataag cacggtagcg ggtggttatt
    13141 gcctgcaatt aaagatatag agccgaacac atatgccttt tacacttggt caacgctgga
    13201 tcagcgatac agaaagcgaa ttgggacttg gaaccgttgt cgcggtggat gcgcgaactg
    13261 tcactttact tttcccatct actggtgaaa accgtctgta cgcacgcagt gattcccccg
    13321 tgacccgcgt gatgttcaac cctggtgata ccattaccag ccatgacggc tggcagatgc
    13381 aagtcgaaga agtaaaagaa gaaaatggct tgctgaccta tatcggtact cgcctggata
    13441 ctgaagagtc cggcgtagcc ctgcgtgaag ttttccttga tagcaaactg gtgttcagca
    13501 aaccgcagga ccgcctgttt gccgggcaga ttgaccgtat ggaccgcttt gcgctgcgtt
    13561 atcgcgcgcg taagtattcc agcgaacagt tccgtatgcc gtacagcggc ctgcgcggtc
    13621 agcgtaccag cctgatcccg caccagctca acatcgctca tgatgttggc cgccgccacg
    13681 cgccgcgcgt cctgctggct gacgaagtgg gtttagggaa aaccattgaa gccgggatga
    13741 tccttcatca gcaactgctc tctggcgctg ctgaacgtgt gctgattatc gtcccggaaa
    13801 ccttacagca tcagtggctg gtagaaatgc tgcgccgttt caacctgcgc tttgcgctgt
    13861 ttgatgatga gcgttatgcc gaagctcagc acgatgctta caacccgttc gacaccgaac
    13921 agctggtgat ttgctcgctg gattttgccc gccgtagcaa acagcgtctg gaacatctct
    13981 gtgaagccga gtgggacctg ctggtggtcg atgaagcgca tcacctggtg tggagcgaag
    14041 acgcgccgag ccgcgaatat caggctattg aacaactggc agagcacgtg ccgggcgttc
    14101 tgctgctgac cgcaaccccg gaacagctgg gaatggaaag ccacttcgcc cgtctgcgtc
    14161 tgctggaccc gaaccgtttc cacgatttcg cgcaattcgt tgaagagcag aaaaattatc
    14221 gtccagtagc ggacgccgtt gccatgctgc tggcaggtaa caaactgagc aatgacgaac
    14281 tgaacatgct tggcgagatg atcggcgagc aggatatcga gccgctgttg caagcagcaa
    14341 acagcgacag cgaagatgcc cagagcgccc gtcaggagct ggtttcgatg ctgatggatc
    14401 gccacggcac cagccgcgtg ctgttccgta acacccgtaa cggtgtgaaa ggcttcccga
    14461 aacgcgagct gcacaccatt aagctgccgc taccgacgca gtatcagacg gctattaaag
    14521 tctccggcat tatgggcgca cgtaaaagtg cggaagaccg cgcccgcgat atgctctacc
    14581 cggagcgtat ttatcaggaa tttgaaggtg ataacgccac ctggtggaac ttcgatccgc
    14641 gcgttgagtg gctgatgggc tacctgacca gccatcgctc tcagaaagtg ctggtgatct
    14701 gcgctaaagc tgccactgcg ctgcaactgg agcaagtact gcgcgaacgt gaaggtattc
    14761 gcgctgcggt gttccacgaa ggtatgtcga ttatcgaacg tgaccgcgct gccgcctggt
    14821 ttgccgaaga agacaccggc gcacaggtac tgctgtgctc agaaatcggt tctgaaggac
    14881 gtaacttcca gttcgccagc cacatggtga tgtttgacct gccattcaac ccggatctac
    14941 tggagcagcg tattggtcgt ctggatcgta tcggccaagc gcacgatatt cagatccatg
    15001 tgccttatct ggagaaaacc gctcagtcgg tgctggtgcg ctggtatcac gaaggtctgg
    15061 atgcatttga gcacacctgc ccgaccggac gcactattta cgatagcgta tacaacgatc
    15121 tgattaacta tctggcttca ccggatcaaa ccgaaggctt tgacgatctg atcaaaaact
    15181 gccgcgagca acatgaagcg ctgaaagcac agctggaaca gggtcgtgac cgcctgctgg
    15241 aaatccactc caacggtggc gaaaaagccc aggcactggc agaaagcatt gaagagcagg
    15301 atgacgatac caacctgatc gccttcgcca tgaacctgtt cgatattatc ggtatcaatc
    15361 aggacgatcg cggcgacaac atgatcgtgc tgacgccgtc cgatcatatg ctggtgccgg
    15421 acttccctgg cctgtcggaa gatggcatca ccatcacctt tgatcgtgaa gtggcgctgg
    15481 cgcgtgaaga tgcgcagttt attacctggg aacatccgct gatccgcaac ggtctggatc
    15541 tgattctttc tggcgatacc ggtagcagca cgatttcact gttaaaaaac aaagcgttgc
    15601 cggtaggtac gctgttggtg gaactgattt atgtggttga agcccaggct ccgaagcagt
    15661 tgcagctcaa ccgcttcctg ccaccgacgc cggtacgtat gctgctggat aaaaacggca
    15721 acaacctggc ggcgcaggta gagtttgaaa cctttaaccg ccagcttaac gcggttaacc
    15781 gtcacaccgg cagcaaactg gttaacgccg tgcagcagga tgttcacgct atccttcaac
    15841 tgggtgaagc gcagatcgag aaatctgccc gtgcattgat tgatgcagca cgtaacgaag
    15901 ccgacgaaaa actgtctgcc gagctgtctc gtctggaagc tctgcgtgca gtgaacccga
    15961 acattcgtga cgacgaactg accgccattg agagcaaccg tcagcaggta atggaaagcc
    16021 tggatcaggc aggttggcgt ctggatgccc tgcgtttgat cgttgtaacg catcagtaac
    16081 ggagccgaaa atggggatgg aaaactacaa tccaccgcag gaaccctggt tggttatcct
    16141 gtatcaggat gaccatatta tggtggtcaa caagccgagc ggtttgttgt ctgtgccggg
    16201 tcgtctggaa gagcacaaag acagcgtgat gacgcgcatt cagcgagatt atccgcaggc
    16261 agaatcggtg catcgtctgg atatggctac cagcggcgtg attgtagtgg cgctgaccaa
    16321 agccgcggag cgggagttaa aacgccagtt ccgcgagcgc gtgccgaaaa agcagtatgt
    16381 gacccgcgtc tgggggcatc catcccccgc agaaggtctg gtggatctgc cgctgatttg
    16441 cgactggcca aaccgcccga aacagaaagt ctgttacgaa acgggtaaac ctgcgcagac
    16501 ggaatatgaa gtggtggagt atgcggcgga taacacggca agagtggtgt taaaaccgat
    16561 taccgggcgt tcgcatcaat tgcgtgtgca tatgctggcg ctggggcatc cgattctcgg
    16621 cgatcgtttt tatgcatcac cagaagcgag agcgatggca ccgcgtttgt tgctacatgc
    16681 agagatgttg acgattaccc atccggcgta tggcaatagt atgacgttta aagcgccagc
    16741 ggatttttaa cctttcctgt acgtttatac cctcatccta agggctgcct cttgtacata
    16801 aatccccgag tccggggatt ttttgtttct gccaaactgc tttcagttgt tgcgtgtggc
    16861 tacgctttcc attcggtaat tcccttcctg ctccacagcg aatttttgaa ctttttcttc
    16921 acccgttgac cgtctcttat atttcatttt atcgggtctg ttatggtcct ttctgattgt
    16981 tattcatggg ctaattagca gtctggttgg cgatgcccat ctcggtgatc cgcgtcgtac
    17041 acgacgcctt gcgtcactag cctcttctct agctcatcat gcaggtcttt ctaattgtga
    17101 aatcatccca cgctactgct aaggttgaag gggcgttcca gccccttcaa ccttagctta
    17161 ttctggctca ttcaataatt gcgttgtcag taatttgttc agcagtactt tgtttagtag
    17221 caacttcttc agtatcaatt tttttggggg tgattttatt aataataatt acctcatcat
    17281 ataggtgggt agcaccgtcc acggtagcac cgtccagggt agcaccgtcc agggtagcac
    17341 cgtccaggtt agcattccac ccaggttcca agtaaaggac tttattgtga cctttcattg
    17401 ttttgagacc aaggtaacaa tccctcatgt caacgccggg aaggtaagcc tctctaagat
    17461 caacgcccaa gttacctggg cctttatgca gctgcaacgt ctgagtacat atctccttat
    17521 tttcaataaa aaaagtgaca cttaactcga tatcaccgaa ggtctgtgcg acccagctca
    17581 tattgccctt tgcgaccatc tcatccggat tttttgcgca tgttttgagt tcattgaata
    17641 attccagact atggataaac ttttctttct cattctcgtt gacagcccca gtaaagtatt
    17701 ttgaggcgtt gatgttgcca gcctttttat tcacgaaatt gaacagaagt ttataagcct
    17761 cagtctgttt atctgaccag aacagatgac gtaacgtctc ccacaagcgt tgaaagatat
    17821 tcatcttcat gtccatcttg tccccgctca ccatcttata aatatcgtta ttgctcatat
    17881 ttaaaagtgt agccggcatg cctggaactg atactttcat ggtaatagcc tcgcattgag
    17941 ttaaaaactc atgatgcgcg ctctgaataa atatactttc aaaaatcgct cattcagata
    18001 tgtgtttttt atttttaaga cgtaaaaata taattccgct cgccgtaaag ctctcaacct
    18061 taagcagggt caacgcgata aaaccaacag aaaccctccc gtaaaccgta tattgagcct
    18121 gtccaccatt ctgagtaaac gccttttctc aaagaagtga ccgccagggc ggccaccttt
    18181 tatcgtgggc agttactcag catcatttac agggtcattt aaaccctttc tgctgcttaa
    18241 tcagctcata ggcctgctga atttcctgcg ctttctgctt cgccatctcc atcatctcag
    18301 gcggcaaacc tttcgccacc agcttatcgg gatggtgttc actcatcagc ttacggtagg
    18361 cacgtttgat ggtggtcgca tcatccgtcg gcttcacgcc cagcacatta caggcatctt
    18421 ccagcgttgg gccacgctgc gcttgctgcc agttaccacc gccagattgc tgctgataac
    18481 cgccgccaaa ctgtgcaccg ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg
    18541 agatccctaa ttcttcagca atgacataca gcaccgcccg ttcattcggg tgcagtgaac
    18601 catcagcaaa cgccgcctga atctggatct ccagaaacat acgaattaag tcaaaacgac
    18661 caaagcagac actgcgaaac tggcgcatct tttcgcgcag cgggtaattg tctgattttc
    18721 ccacccggaa cgcattttgc gccgcagtac gggaagcgcc atgaagattc attcggtcca
    18781 tcaactggct ggcgatatga atatcagcct ccgtgacgcg acctttggat ttggttaaat
    18841 gccccatcac ttcaaaagtg gtggcaaaaa acagcgcctg acgctcacgc tggttggcga
    18901 accacgccat tttacggcta cgggctttat caaacatatg gccaattaac aggcctaaca
    18961 ctacgcccca aaagccgccg cccatcagta aggccacggc cacgccaatg atttttcccc
    19021 aatactgcat atattcccca aatcgacaca cggatatcag ggctatctcc cacaatataa
    19081 aggtgctttt accgttttcc ggctgcggtc aattgtggga catcgcctat aatttgcatt
    19141 atcatacctg tcattcaatg ccgtgcctaa caccactgac gctattcgga caggattaac
    19201 actagcgtag agatgacgag tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg
    19261 atgatggaac aataaaatca acgtatgaaa aaacgtatcc ccactctcct ggccaccatg
    19321 attgccaccg ccctttatag tcaacaggga ctggcagccg acctcgcctc acagtgcatg
    19381 ttgggcgtgc caagctatga ccgtcctctg gtacagggcg ataccaatga cttacccgtg
    19441 actatcaatg ctaaccacgc gaaaggggac tacccggatg acgccgtgtt tactggcagc
    19501 gtggatatca tgcagggtaa cagccgtctg caggccgacg aagtgcagct ccatcaaaaa
    19561 gaggcaccag gacaaccgga gccggtacgt accgttgatg cgctcggtaa tgtccattac
    19621 gacgataacc aggtgatcct caaagggccg aaaggctggg cgaatctgaa caccaaagat
    19681 accaacgtct gggaaggtga ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac
    19741 ctgatgaaac aacgtggcga aaaccgctat accattctgg ataacggtag ctttacctcc
    19801 tgtctgccgg gttctgacac ctggagcgtg gtaggtagcg aaattattca tgaccgcgaa
    19861 gaacaagttg cggagatctg gaacgcccgc tttaaggtgg gtccggtatc gatcttttat
    19921 agcccctatt tgcagttgcc ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac
    19981 gccaagtaca ccaccaccaa ctactttgag ttctacctgc catattactg gaacatcgcg
    20041 ccaaatatgg atgccaccat cacgccgcat tatatgcatc gtcgtggcaa catcatgtgg
    20101 gagaacgaat tccgctacct ctcccaggcg ggcgctggct tgatggaact ggactatctg
    20161 ccttcagata aagtctatga agatgaacac ccgaacgatg acagttcacg tcgttggtta
    20221 ttctactgga accactccgg ggtcatggat caggtgtggc gtttcaacgt cgactacacc
    20281 aaggtcagcg atcctagcta cttcaatgat ttcgataaca agtacggttc cagtactgac
    20341 ggctacgcaa cgcaaaaatt cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt
    20401 tcaaccaagc agttccaggt tttcagcgaa cagaacacca gtagctactc ggcagagccg
    20461 cagttagacg ttaattacta ccagaatgat gttggtccgt ttgatacgcg tatttacggc
    20521 caggcagtgc actttgttaa caccagagac gacatgcctg aagcaacccg tgttcacctg
    20581 gaaccgacca tcaatttgcc gctctctaat aactggggca gcatcaatac cgaagcgaag
    20641 ttgctggcaa cccattatca gcaaaccaat cttgactggt ataactccag aaacacgacc
    20701 aagctggacg aatccgttaa ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc
    20761 tttgaacgcg atatggaaat gctggctccg ggttataccc aaacgctgga accgcgcgcg
    20821 cagtatttgt acgtgccgta tcgcgatcag agcgacatct ataactacga ctcgtctctg
    20881 ctgcaatctg actactctgg cctgttccgg gaccggactt acggcggtct tgaccgtatt
    20941 gcctccgcta accaggtgac gaccggtgtc acatctcgca tatatgatga tgctgccgtt
    21001 gaacgtttta atatttccgt tggtcaaatc tactatttca cggagtctcg cactggcgat
    21061 gacaacataa catgggagaa tgacgacaaa acgggttcac tggtgtgggc aggcgatact
    21121 tactggcgta tctccgagcg ttggggattg cgtggcggga ttcagtacga tacacgtctg
    21181 gataacgtag cgaccagtaa ctccagcatt gaataccgtc gggatgaaga ccgtctggta
    21241 cagctgaatt accgttacgc cagcccggaa tatattcagg ctacgctgcc taagtactat
    21301 tccactgctg agcaatataa gaatggtatt tcgcaggtag gtgctgtcgc cagctggcca
    21361 attgccgatc gttggtccat tgttggggcc tactactacg acaccaatgc gaacaagcaa
    21421 gccgactcta tgttaggtgt gcaatacagc tcctgctgct atgcaattcg cgtcggttac
    21481 gagcggaagc tgaacggttg ggataacgat aaacaacatg cggtatatga caacgcaatc
    21541 ggctttaaca tcgaacttcg cggcctgagc tccaactacg gtctgggtac gcaagagatg
    21601 ctgcgttcga acattctgcc gtatcaaaac actttgtgat ttgttgattt accacgtaat
    21661 ccgcagtgcg gttaattgaa atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg
    21721 tatcgccatg atcgcgaata ccagtttcgc tgccccccag gtagtcgata aagtcgcagc
    21781 cgtcgtcaat aacggcgtcg tgctggaaag cgacgttgat ggattaatgc agtcggtaaa
    21841 actgaacgct gctcaggcaa ggcagcaact tcctgatgac gcgacgctgc gccaccaaat
    21901 catggaacgt ttgatcatgg atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa
    21961 aatctccgat gagcagctgg atcaggcgat tgctaacatc gcgaaacaga acaacatgac
    22021 gctggatcag atgcgcagcc gtctggctta cgatggtctg aactacaaca cctatcgtaa
    22081 ccagatccgc aaagagatga ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat
    22141 caccatcctg ccgcaggaag tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc
    22201 cagcactgag ctgaacctga gccacatcct gatcccgctg ccggaaaacc cgacctctga
    22261 tcaggtgaac gaagcggaaa gccaggcgcg cgccattgtc gatcaggcgc gtaacggcgc
    22321 tgatttcggt aagctggcga ttgctcattc tgccgaccag caggcgctga acggcggcca
    22381 gatgggctgg ggccgtattc aggagttgcc cgggatcttc gcccaggcat taagcaccgc
    22441 gaagaaaggc gacattgttg gcccgattcg ttccggcgtt ggcttccata tactgaaagt
    22501 taacgacctg cgcggcgaaa gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat
    22561 tctgctgaaa ccgtcgccga tcatgactga cgaacaggct cgtgtgaaac tggaacagat
    22621 tgctgctgat atcaagagtg gtaaaacgac ttttgctgcc gcagcgaaag agttctctca
    22681 ggatccaggc tctgctaacc agggcggtga tctcggctgg gctacaccag atattttcga
    22741 tccggccttc cgtgacgccc tgacccgcct gaacaaaggt caaatgagtg caccggttca
    22801 ctcttcattc ggctggcatt taatcgaact gctggatacc cgtaatgtcg ataaaaccga
    22861 cgctgcgcag aaagatcgtg cataccgcat gctgatgaac cgtaagttct cggaagaagc
    22921 agcaagctgg atgcaggaac aacgtgccag cgcctacgtt aaaatcctga gcaactaatg
    22981 gttaaaaccc aacgtgttgt gatcactccc ggcgagcccg ccgggattgg cccggactta
    23041 gttgtccagc ttgcacagcg tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact
    23101 ctccttaccg accgggcagc gatgctcggt ttgccgctca ccctccgccc ttattccccc
    23161 aactcccctg cacaaccgca aactacgggc acattaacgc tacttcctgt cgcgctacgt
    23221 gaatctgtca ctgcggggca gttagcgatt gaaaatgggc attacgtggt ggagacgctg
    23281 gcgcgagcgt gtgatggctg tctgaacggt gaatttgctg cgctgatcac aggccccgtg
    23341 cataaaggcg tcattaacga cgcaggcatt ccgtttaccg gtcataccga gtttttcgaa
    23401 gagcgttcgc aggcgaaaaa agtggtgatg atgctggcga ccgaagaact tcgcgtggcg
    23461 ctggcaacga cgcatttacc gctgcgcgat atcgcagatg ctatcacccc tgcacttttg
    23521 cacgaagtga ttgctatttt gcatcacgat ttgcgtacaa aatttggtat tgccgaaccg
    23581 cgcattctgg tctgcgggct gaatccgcac gcgggcgaag gcggtcatat gggtacggaa
    23641 gagatagaca ccattattcc tgtgctcgac gagctgcggg cgcaggggat gaaactcaac
    23701 gggccgctgc ctgccgatac cctgtttcag ccgaaatatc tcgataacgc cgacgccgtg
    23761 ctggcgatgt accacgatca gggtcttccc gtgctaaaat accagggctt cgggcgcggt
    23821 gtgaacatta cgctgggcct gccctttatt cgcacatcag tggaccacgg caccgcactt
    23881 gaactggcgg gacgtggcga agccgatgtc ggcagtttta ttacggcgct taatctcgcc
    23941 atcaaaatga ttgttaacac ccaatgaata atcgagtcca ccagggccac ttagcccgta
    24001 aacgcttcgg gcaaaacttt ctcaacgatc agttcgtgat cgacagcatt gtttctgcca
    24061 ttaacccgca aaagggccag gcaatggtcg aaatcggccc cggtctggcg gcattgaccg
    24121 agccggtcgg cgaacgtctg gaccagctga cggtcatcga acttgaccgc gatctggcgg
    24181 cacgtctgca aacgcatcca ttcttaggcc cgaaactgac gatttatcag caggatgcga
    24241 tgacctttaa ctttggcgaa ctggccgaga aaatgggtca gccgctgcgt gttttcggca
    24301 acctgcctta taacatctcc acgccgttga tgttccatct gtttagctat actgatgcca
    24361 ttgccgacat gcactttatg ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga
    24421 acagcaaagc gtatggtcga ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg
    24481 tactggaagt tccgccgtca gcctttacac caccacccaa agtggattcc gccgtcgtgc
    24541 gcctggttcc tcatgcaacg atgcctcacc cggttaaaga tgttcgtgtg ttgagccgca
    24601 tcaccaccga agcctttaac cagcgtcgta aaaccattcg taacagcctc ggcaacctgt
    24661 ttagcgtcga ggtgttaacg ggaatgggga tcgacccggc gatgcgagcg gaaaatatct
    24721 ctgtcgcgca atattgccag atggcgaact atctggcgga gaacgcgcct tttcaggaga
    24781 gttaacgatg atcaattcgc cccgagtgtg tattcaggtt caaagcgtct acattgaggc
    24841 acaatcttca cctgataatg aacgttacgt ttttgcttat accgtaacca tacgcaatct
    24901 ggggcgagcg ccagtgcagt tgttggggcg ttactggctg atcaccaatg gcaatggccg
    24961 tgaaaccgaa gtccagggcg aaggagtggt tggcgtccag ccacttatcg cgcctggcga
    25021 agagtaccag tacaccagcg gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca
    25081 ctacgaaatg atcgatgaaa atggcgtccc tttcagcatc gacattcccg tattccgact
    25141 cgccgttccc acactcattc attaaaagaa tatggcgaca taccttattg gcgacgttca
    25201 tggttgttac gatgaactga tcgcattgct gcataaagta gaatttaccc ctgggaaaga
    25261 taccctctgg ctgacgggcg atctggtcgc gcgcggcccg ggttcgctgg atgttctgcg
    25321 ctatgtgaaa tccttaggcg acagcgtacg tctggtgctg ggcaatcacg atctgcatct
    25381 gctggcagta tttgccggga tcagccgcaa taaaccgaaa gatcgcctga caccgctgct
    25441 ggaagcgccg gatgccgacg agctgcttaa ctggctgcgt cgccagcctc tgctgcaaat
    25501 cgacgaagag aaaaagctgg tgatggccca cgcagggatc acgccgcagt gggatctgca
    25561 gaccgccaaa gagtgcgcgc gcgatgtaga agcggtgctg tcgagtgact cctatccctt
    25621 ctttctcgac gctatgtacg gcgatatgcc aaataactgg tcaccggaat tgcgggggct
    25681 gggaagactg cggtttatca ccaacgcctt tacccgtatg cgtttttgct tcccgaacgg
    25741 tcaactggat atgtacagca aagaatcgcc ggaagaggcc cctgccccac tgaaaccgtg
    25801 gtttgcgatt cctggacctg tcgctgaaga atacagcatc gcctttggtc actgggcatc
    25861 gctggaaggc aaaggtacgc cggaaggtat ttacgcgctg gataccggct gctgctgggg
    25921 tgggtcatta acctgcctgc gctgggaaga taaacagtat tttgtccagc cgtcgaaccg
    25981 gcataaggat ttgggtgagg gagaggcggt cgcgtcttaa atcccgtcga gcataaaatc
    26041 taatgctcct ttaatcaacg ttcggtgcgc gctggcatcg caaaatttcg ttcccagcgc
    26101 gttaactgga acagttgcag tctcatgcgt catgagcaca tattctttgc cgtctaccag
    26161 taaaaggatt gggttaagcc gttctggtgg gcgaatacgg ctaaatcgtt caataggtgt
    26221 taatggaata acaatacggc gattaatcac accaataatg tcgctggtaa catcgataac
    26281 aaagggaaac gcggcgttcc tgctgcgact gcggtatacc gtaaattgca tcgctagaag
    26341 ctccggtact catcagagaa acatccggtt tcatcagcca attgatttaa cgcagcgata
    26401 gcttcagcgt tctcttcacg ccaacgtgtt gctgcatgtt ttttaagttc agcattgagc
    26461 gcaaccgtaa gggtggcgct catatttaag cctgcatcac gagcgcgatt gactaactca
    26521 cggtcgacgg tgacggtcac actttgtgtg gtacgttttg cagtcatgat atcgtgctcc
    26581 tgtacaattt atacgtctag tatacacgga tatctgcacg tatcagtgat gccggaattc
    26641 taatatacaa aattaccgcc gctccagaat ctcaaaacaa tagctgtgag agttctgcgc
    26701 atcagcgtcg tggaattcgc tgaataccga ttcccagtca tccggctcgt aatccggaaa
    26761 atgggtgtcg ccttccactt ctgcgtcgat atgcgtcagg tacagttttt gcgctttcgg
    26821 caggaactgc tcataaacgc gaccgccgcc aatcaccatg atttctggta cgtcaccaca
    26881 cgccgcgatg gcttcatcca ccgacttcac ccacgttacg cgatcgtccg tacccggttg
    26941 actgctgagg ataatatttt tgcgtcctgg caacggacga ccgatggatt ccctggtatg
    27001 gcggcccata attacgggtt tatttaaggt gttgcgttta aaccaggcga gatcggcagg
    27061 caggttccac ggcatggcgt tttccatgcc gataacgcga tctaccgcta acgccgcaat
    27121 cagactgatc attgagattt cccgataaaa aaaattgtcg ccactatacg taaagcgtaa
    27181 accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt ttatattctg ctggcgagtc
    27241 cacgctctct ccctggactc gccgcattac aatgaaacaa aaacaaacag ttagctgtaa
    27301 agtgtgattt acgtcactct ttattaggat gagggtttcg tttccggttc atccgccatg
    27361 ttgccggtat gtttaccttc ttctgttccc tgccagccat gtcgttgaat taatgacaga
    27421 tgttcgcggt cctcggtgat gatctcactt aacatcgcgc tggtgcgttt atagaccgcc
    27481 gcgcgggctt tggtgtcatt ctcaaccatt gccatctctt ccaccatctg aatattaaag
    27541 cggcggaaca catcggcacg ttctcgcgct tcatacggcc ccagacctaa actttccagc
    27601 gccagacgcc cggttttcag cgcaccttca aaggtttcac gctctggctt ttcaacgcct
    27661 gcctgacgca aacaaatgta gtgatcgaca tcgcgggcgc gggcaataat ctgcaaatgc
    27721 gggaaatgtt ctttcaccat ctccgtcagt tgcaggttgg tttgcggatc gtcaatggcg
    27781 ttaatcagca cttccgcttt cgccgctccc gcagattcca gtaaatccat ccgcgtggca
    27841 tcgccataaa acactttcat accaaattta cgcagcgttt cgatatggtc cggatcgtga
    27901 tcgagcacca ccattttcac cccgctggag agcagtaaac gtccggtgat ctgcccgaaa
    27961 cgaccgaatc cggcaatgat tacccgcggc tgttcttcgt cgatctcatc ggcttcacgc
    28021 gcctcttcag tagaagattg ctcaaggcga ttgaggatca ccagcagaat cggcgttgcc
    28081 gccatcgaca gcgccaccgc cagggtcagc gatttcgccc actccggctc cagcacattc
    28141 gccatctgcg ccgcgccaaa taccacaaag gcaaactcac tgccctgccc taacaacacc
    28201 gcaaaccagc gacgctgttt atttggcacc tgcaacggtc gggcaatcag ccacagcatg
    28261 gcgattttga tgatgaggaa accgagcagc aaaatgacaa tgcgcaatgg gttttcaatc
    28321 agcgtgccaa agtctatcga catgccaaca ccgatgaaaa acagccccaa cagcaaacct
    28381 ttaaagggtt cgatatcgct ttccagcgca tgacggtatt ccgagcttgc cagcagtacg
    28441 cccgccagaa acgcgcccat cgccatcgac aagccgacct cttccagcag caaaccaaag
    28501 ccaaacacaa ggaataacgc cacggcacta aacacttccc gcaagccaga gcgggcaaca
    28561 aaacgcagcg ccggacgcgt gacatagcgc cccagcagta ccaccagcac cagcgcgccc
    28621 gccactttta acgccgagag agcaaatgcg cccatcgtcg tcgaggcact gctcgccgcc
    28681 agtagcggga tcatcgccac cagcgggatt gccgcgatat cctggaacag cagcaccgca
    28741 aaggcactgc ggcccatttg cgtcaccatc agattgcgtt cattcatcgc ctgcatggca
    28801 atcgccgtag aggagagcgc cagcgtcatg ccgatcaatt ccgcgacctg ccagcgcagc
    28861 ccaagtaaca tacagaacag ccccagcagg ccgccgcaaa tcaccatctg caatgcgcca
    28921 ccgccaaaca ctgccgcacg cagcttccac agcctttgtg gatcgagttc gaggccgata
    28981 ataaacagca tcagcaccac gccaatctcg gcaaagtgca gaatagattc ggcatcggtc
    29041 accagtcgca gcccccacgg gccaataatg cagccggcga tcaggtagcc aagtaccgat
    29101 cccagaccaa gacgtactgc aatgggtaca atcagcgccg ccgaaccgag ataaatcagc
    29161 gcctgaacca gcgtatggct atccatgatg ggcctcctgc cattccagca gacgttgctt
    29221 atagtgacgc gcctgccctt cgagggtttc gtcgtcacaa ataaaggtgc agtgcatggc
    29281 aaacggtggc agccagttca gcccgcagta gattgccgtc gcctgtagcg gctgcgacag
    29341 cacatcaaag cccggatgcg caccaatttc aaaatggctt tccccgccgc cggtcgtcac
    29401 cgcccacagc aaatgtttgc catgcagcgc cgtgccgccg tgaccgtaag cccagccgtg
    29461 cgagaagact ttatcgatcc aaagtttaag gagcggagga atgctgtacc actgcatcgg
    29521 gtgctgccag acgatcagat cggcgcgaga cagcgcctcc tgctcggcgg caatatcgat
    29581 attgaagtca ggatagagtt gataaagaga gcgaatttcg acgccttcca gcgtccttgc
    29641 ctgttcaaac atccgtttat tcgcatggga atgatgcgga tacggatgcg cataaattat
    29701 aagaatcata ggtagcccgc gtgtttacgc ctttgttttc ccacaaagtg tagtcatata
    29761 ttcacgcggc ttatagtcaa taatattgat tcagaaaaga gataaatctg aacaaattac
    29821 cccattctct gcccgcggat cgtcatattg ctggtctgcg ccagtgacat ccctcgagtc
    29881 tccggggcaa acgctacgga aatcaacaag ccaaacagcg agatacccgc ccccattagc
    29941 atcgtgttac tgataccgta attattgata aagatcggta gtgcccaggt cgaaacaatg
    30001 gtgccaatac gacttaagga cataatcacg cccacggcag aggcgcggat atccgtcggg
    30061 aagagttcat taggatagag ccactgcaaa ttacccggcc cgccagagaa aaaggcatac
    30121 accgctaagg ccatcactac cagccagatc cccatatccg ggatcagccc caaaaccgcc
    30181 agcgccagcg tcatcatggc aaagctgcca atcaacaatg gacgccgtcc ggcagtgttt
    30241 agccacagca tcggcggaat acagccgagc ataaagaaca ggctaatcac cacgttcccc
    30301 agtgccgcgt ttttgccaac acccaatccc aacaaaccaa cgatttgcgg gccaaaggtg
    30361 taaatggcga acattgggat cacctggcag gtccagatgg cggcaacaaa cagaacaaaa
    30421 ggaaaatggc ggcgattaaa cagatcgcga aaacgggttt gctgcggctg ctcttcatcg
    30481 aaagccaccg gttcgccaaa cagtttgatc atcatctctt cgcactcttt tactcgccct
    30541 ttgcgtaata accagcgggg agattcaggc agttcgaatc gaccaatcaa aatcaacaaa
    30601 caggggatcg ccgcgctacc cagcatccag cgccagccgc cttccacatc ataaagccag
    30661 tagccgacca gatcggcaca ggtcgcgccg acataccaca tggcggcgat aaagctgatg
    30721 gaaaacgccc gctgacgggt actggagaac tcagtgatca tcgaagtggc gatgggatag
    30781 tctgcaccga tgacaatgcc gataagtacc cgcatcacca acagttcgac gggggatgaa
    30841 acaaacatcg tcgccaccga tatcacgccg atggcgatga tatcaatgag gaacattttg
    30901 cgccgtccga ctttatcgga aatataacca aacagcgatg tgccaacgaa cagcccggcg
    30961 agcgttcccg cgcccagcaa gccaatccag tcagcgtcca gttttagcgc cggcgtcagt
    31021 tgctccagcg ccacgccaat cattaccagt atataaccat ccagaaacgg accaccgctt
    31081 ccccacagca aaatgcggcg gtgaatagag gaaaatttaa gatcgtcaaa gtttctggac
    31141 ggttgcatgg gttattcctg ttttttgcaa tgagagtcag tgcggcgtta cgtatcaaac
    31201 caacatcagc cgtaacggaa ctccacacca aaggtgccgc gcgggtattc ccactgttcc
    31261 agcgccgacc ccagccccag aatgcgacag gtgccgcact ccagacatcc ggcgtaatcg
    31321 aagcgcacac tgccgtcatc ctgcttcttg tacagacctg cggggcacgc tttcaccagc
    31381 agctccagcg cctgtttatc ggcatcggct ttcacaacaa tgtgcggatg ctcttcatcg
    31441 acattgaatt tattgacgcc cagtttgacg tccacattga cgggagaagt cataaaacgg
    31501 tcactccttt catgccatcc ttgatcagat tgatgaagcc cactttcttg ccgtggcgga
    31561 ggattttctt gcgcatcagt tccggcgcac tgccgtcaat ggtgaacagg tcacgcgcca
    31621 cgcctaccgc cagttccggg tagccgctaa acatgcgtgg gttatcaagg aaggccggta
    31681 gtttctggta catacgcata tcgcgcagcg ggccactctc aagatgctga cgatattccg
    31741 cgagtttttg cttactgaaa tcgtcgcttt tcatcgctga aagcacggtt tttgctgcag
    31801 cttccccggc ggcaatcgcc agatccatac cgcgaatggt aaaaccgagg ttcatacaca
    31861 ttccggcggc atcaccggca atcaatacgc cgtcaccgac cagctccggc agcatgttga
    31921 tgcctgcttc cggcactacg tgagcggaat attccaccag cttgccaccc gcgatcagcg
    31981 gtgcaacggc cggatgttgt ttgaaatctt ccagcatttg cggcaccgat tttttcgcgt
    32041 catgcagatg atgcagacca caaaccagcc ccagcgacag ggtgttttca ttggtataaa
    32101 gaaaaccgcc gcccatcagg ccatcggtgg gtgatcccgc aaataggcaa gccgccccct
    32161 gattaccctg caactgaaaa cggtcttcaa taaccgactt cggtaactcg atcagttcct
    32221 tcacgccaac cgccacattc gtcggtttaa cgcgttttgc catccccagc ttttcggcaa
    32281 ggatggagtt cactccatca gcaaggatca ccgttttcgc ttcaatcaca tcgccatcgg
    32341 cttctacacc gacgactttg ccatcgcgct gtacgaggtt atcgacgcgg atcccggtaa
    32401 ttaactgcgc gcccgcttct tcggcctgct ccatcagcca ggcatcaaat ttactgcgca
    32461 aaacggagta agaacgctgg gatggcgagg tttcgtcacc gttgcagtag tccatagtca
    32521 tcgctgactt ttccgtcata aacgcgagtt tttcatgggt gatcaggcgt tctaccgggg
    32581 cggattcggc gaaaccagga ataatgtgtt ccaggctgtg ggcatagaga cgcccgccgg
    32641 tgacgttctt ggcacctgcg gaattgccac gctcgataac taacacttgc gccccttcgc
    32701 gggcgagcac cagtgcggca accgaaccgg caagccctgc accgacgatg atggcgtcaa
    32761 agatatcttc ggacataaga gctccctgtc agcggcagga ccaaaatgca gccctgccag
    32821 agtggatcaa cgcgctaaag ctgcggtcag cgccggaagg atcttcacgg cgtcgccaac
    32881 aatgccgtaa tccgcgtact ggaagatcgg cgcattttta tctttattga tggcgaaaat
    32941 ggtttgcgac gcgttagcgc caaccatgtg ctggatctgc ccggagatcc ccacagccag
    33001 gtacagttca ggtttcagca tcaggttgga gataccgaca tagcgttcgt gctccatcca
    33061 tttttcgttt tctgccaccg gacgagaaca ggccaactcc gcacctatcg ccttgcaaag
    33121 ctgttctgcc agcgcaatgt tctctttgct gccaataccg cgaccgacgc tgaccaccag
    33181 gcgggctttg tcgagatcga cgctgttgct ctggcgcgcc tgggttgccg tgcgggtaat
    33241 cgccacagcc ggagcctgcc actccacggt gtgcgtttcg ccagtgcgtg acgcgtctgg
    33301 ctgagccgca tcgaacgtgc cgctgctgat ggtcagtacc gcatacggcg tggcaatgcg
    33361 ttcttcgcca atcgccagac caccgtaaac catatgtttc actgttgctt taccgtcctg
    33421 tacgctgacg gtgctggcat cgttagacac cgccgcttta aggcgataac ccagttttgc
    33481 cgccagtaat ttgccgcgac gggtgtttgg caacagcacc aggccgtctg caccgtgctg
    33541 gcgaatagtg tcagccatga cactggcgta atcttcgatc atccgatcgt ccggtttgcc
    33601 gtttaatttc cagacatgat tagcgccgag ctggattgcc tgtgcgccgt cggcatcatt
    33661 gaggacaaag gcattgattt gattagctaa agcctgcgca ccgttcatca gttccggcag
    33721 acgagaaggg gtatcgctga atacccagac ttgagaaaac gtgttcatag catcccctgt
    33781 aattaaatga ctttgcgaag attttcagca aatgcggcga tctgttcttc gccgtcgcct
    33841 tcaatcacga tgcgctgacg ctcgcgctgt ttcggcgcgg caacctgttg ttctgaccat
    33901 gctgcctctg cgttaaaacc gatatccgcc gccgaccata cctggactgg ctttttcgcc
    33961 gcgccgagaa tggctttcat cgaaggaatt tgtggggagt tgatatcagt ggaaacagca
    34021 acaaccgcag gcagtggaat gcttaaggtt tcggtttcat cttccagttc gcgctcaacg
    34081 gtgagggtat ctgccgtcag ggagataatt ttgctgacgc cgttaactgc cggaatattg
    34141 aggatttcgc ctaccagcaa accaacctgc tgggcataaa ggtcggaaga accatcgcca
    34201 cagaggatca aatcaaagcc tgctttctgg gcggctgcag ccagtgcgct cgccgtttgt
    34261 tgcggcagtg cctgctcgaa ctggtcatca atcaccacaa tcagttcatc cggaccgcgc
    34321 gatagcacat ctttacgccc tttggcgttg gtcagggctt taccgcccac acttagggcc
    34381 gtcacctgcg cctctgctgc ctgttgcttt agctggcaag ccgcttcaat agcgttgaga
    34441 tcgtattggc ttattttggc atcggcttta ctgaagtcta acgaaccatc agcattattg
    34501 accgcaatat cctgttcatc aggcacgcac ttatagcaag taataatctt cattgcatct
    34561 ccagaaatca tgaaggaaac gtcgttcata tcggtggtgt taattacaga tatcatgccc
    34621 tttaatttgg aaataaaact ttaatcacca atattgaaaa tgtcacacgc agataaatcc
    34681 aactttcaat attgttaagt tcctcaccaa tattgaaaac acggcgtagc aaaaagaaat
    34741 tttcaatatt gttttatgga tcaccaatat tgaaagtcag atgctatttt tttactcgca
    34801 acataacata acaaactgat taataatcaa aattaacgag aaaagcgccc tctattcacc
    34861 cgaaacaaaa atgtgataac aatcacagaa tgcagcttat tgaataccca ttatgagtta
    34921 gccattaacg cgtccatgag gttaataata attatattaa atgttaacaa aaataaaaca
    34981 aacgggaacg caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa
    35041 gagaaaaacg ggaatagaac cgaaggtttt ctttccgcca ttaataatcg tcggcatact
    35101 ttgttggctt acagtcagag atctggatgc ggcgaatgtc gttattaatg ctgtattcag
    35161 ttacgtcacc aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg
    35221 ttggttctgg ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccacctga
    35281 atttagtacc gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact
    35341 gttctgggga tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc
    35401 gaactcgaca ggagcgaaag agttggggct ggcttacagc ttgttcctct ggggacctct
    35461 gccgtgggcc acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa
    35521 aatggaagtg attcgcccca gctccaccct agtgccgctg gtaggtgaaa aacacgccaa
    35581 agggttgttc ggcactatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg
    35641 taccagtctg ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat
    35701 tccgcatacc ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat
    35761 ttgcgtcgcc tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct
    35821 gagcttcctg atgctgggtt gggtgttcat cgtcagcggt gccagcttca tcatgaacta
    35881 cttcaccgat tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga
    35941 tcccatcgct aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt
    36001 gatttacgcc atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg
    36061 tgaactgtgc ttcggcatgg tgatgggact gacagcatcc acctggatcc tgtggactgt
    36121 actcggtagt aacactctgc tgttgataga taaaaacatc atcaacattc caaatctgat
    36181 cgaacagtac ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cgctcagcac
    36241 cgccaccatg tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc
    36301 ctgctcttat accctggcga tgtccacttg ccgcgaagta cgcgatggtg aagaaccacc
    36361 gctgctggtg cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttttgctggc
    36421 gctcggcggc ctgaaaccga ttcaaactgc cattatcgcc ggaggatgcc cgctgttctt
    36481 cgtcaacatt atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta
    36541 attaaccccc aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga
    36601 actgtttgtc gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc
    36661 cgagtgcgac cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg
    36721 tatcgacagt ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct
    36781 cgccgccgtg tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagtt
    36841 gccgggcggg ttcaatacct tcctgcgcga aggcacacaa gagcagatcg acaagattat
    36901 ggctttccgc ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg
    36961 ctccgacgtg ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa
    37021 tggtagtaag tgttttatta ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg
    37081 cgacggggct tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc
    37141 gggcatcaaa gtgaccaaac ttgagaagct cggtctgcgt atggatagct gctgtgaaat
    37201 cacctttgac gatgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt
    37261 taaccgcgtc aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg
    37321 tacggcgatg tgcgcctttg aagatgcggc gcgctacgcc aaccagcgcg tgcagtttgg
    37381 cgaggctatt ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt
    37441 aaactccatg aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac
    37501 ctctggcgat gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga
    37561 tagcgcaatg caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt
    37621 ctggcgtgac ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac
    37681 gctgggtcgt gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct
    37741 gacgcgtctt atcaggccta cgggagcacg aatgtaggcc ggataaggcg tttacgccgc
    37801 atccggcagt catgcgctac ataaattttt caggagctaa taatggatca tctacccatg
    37861 ccgaaattcg ggccgttggc cggattgcgc gttgtcttct ccggtatcga aattgccggg
    37921 ccgttcgccg ggcaaatgtt cgcagaatgg ggtgcggaag ttatctggat cgagaacgtc
    37981 gcctgggccg acaccattcg cgttcaaccg aactacccgc aactctcccg ccgcaatttg
    38041 cacgcgctgt cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg
    38101 gaaaccaccg atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt
    38161 accgatgaag tactgtggca acacaacccg aaactggtta tcgctcacct gtccggtttt
    38221 ggtcagtacg gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc
    38281 ttcagtggtt acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat
    38341 accgccgatt acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa
    38401 gcgcgtgaaa ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt
    38461 atgggccagt acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc
    38521 aaaggtaaag atccctacta tgccggttgc ggtctgtata aatgtgccga cggctacatc
    38581 gtgatggaac tggtgggcat tacccaaatt gaagagtgct ttaaagatat tggcctcgca
    38641 catctactcg gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc
    38701 ccttacggcc cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca
    38761 gaagttaaag aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg
    38821 gaactggaaa gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg
    38881 gatggtcgca cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa
    38941 atctggcgcg gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc
    39001 tacagcgaaa acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa
    39061 acgcgtggcg tcagtcggct tgcctgacta gcgcccgccc aataacaaac gaacaaaaat
    39121 ggatagaggt gcaatggata tcattggcgg acaacatcta cgtcaaatgt gggacgatct
    39181 ggcggacgtt tacggtcata aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa
    39241 ccggtatagt tatcttgagt taaatcagga gattaaccgc acggcaaacc tgttttatac
    39301 gctggggatt cgcaaaggcg acaaggttgc actacatctc gacaactgcc cggaatttat
    39361 cttttgctgg ttcgggctgg caaaaattgg cgcgattatg gtgccgatta acgcccgcct
    39421 gttacgcgag gaaagtgcgt ggatcctgca aaatagccag gcgtgcctgc tggtgaccag
    39481 tgcgcaattc tatcctatgt atcaacagat tcagcaagaa gatgccactc aattgcggca
    39541 catttgcctg acagatgtgg cacttcccgc tgatgatggc gtgagttcgt ttactcaact
    39601 gaaaaatcaa caacctgcca ccttgtgcta tgcaccgccg ctatcgactg acgatacggc
    39661 ggaaattctc ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta
    39721 caacctgcgc ttcgctggat attactccgc ctggcagtgt gcactgcgtg acgatgacgt
    39781 ctacctgacg gtaatgcctg cgtttcatat cgattgccag tgtactgcgg cgatggcggc
    39841 gttttctgcc ggggccacct ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg
    39901 acaggtacag aagtaccgcg ccaccattac cgaatgtatt ccgatgatga ttcgtacgtt
    39961 gatggtgcag ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta
    40021 tctcaacttg tcggagcagg aaaaagacac attttgtgaa cgcttcggtg ttcgcttgct
    40081 gacgtcttat gggatgacgg aaaccattgt gggcattatc ggcgatcgcc ctggcgataa
    40141 acgacgctgg ccgtcgattg gtcgggcggg gttttgctac gacgcggaga tccgcgacga
    40201 tcacaatcgc ccgctcccgg caggtgagat cggtgaaatc tgtattaaag gcgtaccagg
    40261 gaaaaccatc ttcaaagagt attttctcaa cccgaaagcc actgcaaaag tgctggaagc
    40321 tgatggctgg ctgcataccg gcgataccgg ataccgcgac gaagaaggct ttttttattt
    40381 catcgatcgc cgctgcaata tgatcaaacg cggtggcgag aatgtctcct gcgtggagct
    40441 ggaaaatatc atcgccaccc atccaaaaat tcaggatatc gtggttgtgg gtattaaaga
    40501 ttcgattcgc gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag
    40561 cgaagaggaa tttttccgct tctgcgaaca aaatatggcg aaatttaaag tgccctctta
    40621 tctggagatc agaaaagatc tgccacgtaa ttgctcgggg aaaataatta gaaagaatct
    40681 gaaataaatg aaacggcagg gaactaccct gccagctaat aatcacgctc taaaacaata
    40741 tgcatttttt gcagggatgt tgtcgtccct gaaaaagcaa aaatggagaa aaggaatgag
    40801 tgaatcatta catctgaccc gcaatggatc aattctggaa attactcttg atcgcccaaa
    40861 agcgaatgct attgatgcaa aaaccagctt tgaaatgggc gaagtatttc taaatttccg
    40921 tgacgatccg caattacgtg tcgccattat taccggtgcc ggagagaaat tcttttccgc
    40981 gggctgggat ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg
    41041 tggttttgcg ggattaaccg aaattttcaa tctcgacaaa ccggttatcg cagctgtgaa
    41101 cggctacgcc tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc
    41161 cgataacgcc agcttcgccc tgccggaagc caaactgggc atcgttcctg acagcggcgg
    41221 tgtgctgcgt ctgccgaaga tcctgccgcc tgccatcgtc aatgaaatgg tgatgaccgg
    41281 cagacgaatg ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca
    41341 ggcggaactg atggataacg cccgcgaact ggctcagcag ctggttaaca gcgccccgct
    41401 ggcgattgcg gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaatc
    41461 gtatcgctat attcgcagcg gcgtgttgaa acactatcca tcggttctgc attcggaaga
    41521 tgccattgaa gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta
    41581 acaaccgtga gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt
    41641 gtccatccca gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc
    41701 ccactcgcct cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt
    41761 caggatggct gcattatgca tggctacacc gacactgaca ccatcgttgg ggaaaacggc
    41821 catatcgggc acggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg
    41881 atgaacagcg tgattatgga tggcgcggtc attggcgaag agagcattgt tgccgccatg
    41941 agctttgtca aagcaggctt tcacggcgag aaacgccagt tgttgatggg tacgccagcc
    42001 cgcgctgtac gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag
    42061 tatcaggatc ttgtcgggcg ctgtcatgca tcgttacatg aaacacagcc actgaggcaa
    42121 atggaggaaa atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct
    42181 tgccggatgc ggcgtaaacg ccttatccaa ctctgctgga gagcaaccgt aggccggata
    42241 agatgcgcca gcatcgcatc cggcgaccag gtcgcacggt atcatcgaaa tgacattaac
    42301 gacgcatact ctttgacaac atcatctgcc acttctgctc gcggttaagt tccgtgagcg
    42361 gaacggcagg aatacgcggt gtctttctta cgccgactaa tttttcccga ctgttattgc
    42421 gcaggcgcgc gtaaatttgc tcatcgatat cgaccacttt aaccagtcgc tgacactggc
    42481 atccccgccc ttccagctta ttagggatca tcttaacttc gcagcttatt tctgtgactt
    42541 ccgacagaat ataagtcagg gtattaaccg ccttgctgtg ttcaatatcg aaatgaatag
    42601 agatctctct tgctatcacc caccgatttt cagccatcat ccattcggcg attaacaagt
    42661 agagtggttt ttcaacatat ccttcacaca tacgaactcc agtttcacaa attaaaagtc
    42721 tggctatgac tcgttttgaa gcaattaaac aaggccatat taaaattgtg gatatatcga
    42781 tagtatgcaa cttcaccgtc gataagtgtg agctaaatcc tgcttatgtc atcaaaaata
    42841 tcgattcccc aaaagattta cttaatgggc aaaaaaaacg gtcctcatca gagaaccgta
    42901 ttagttattc gatcaaatta gcggatgaaa aatatccgcc atgacacgct attatttgat
    42961 ctgtgcgtgc atttcctgca ccgaaattac tttttcagtc gcatcggcat tcagcgccat
    43021 cgcggtagcg aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact
    43081 gcgacggatc acgcgggagt cttcaatcgc acgacggcct gaggtggtgt tgatgatgta
    43141 ggtatattcg ccattcttga tacggtcctg aatgtgcgga cggccttcat gcaccttgtt
    43201 taccagacgc ggattgatac ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc
    43261 cagctcgaag ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc
    43321 ttcgcgcacg gaaagcagcg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg
    43381 cgctttggca aacgcttcag cgaaggtgcg gcccacgccc atgacttccc cggtagaacg
    43441 catttctggc cctaacagcg ggtcaacgcc cgggaattta ttgaacggca gcaccacttc
    43501 tttcaccgag tagtacggcg ggataacttc tttggttacg ccctgctcag ccagcgattt
    43561 gccagccatc acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa
    43621 cggaacggta cgcgccgcac gcgggttaac ttcaatcagg tagacttcgt tgtttttcac
    43681 cgcaaactgc acgttcatca ggccgcgcac ctgcaattcg aaggccagtt tctgcacctg
    43741 ctggcgcatt acatcctgaa tttcctgact taaggtgtac gctggcagcg aacatgcgga
    43801 gtcaccggag tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat
    43861 ttcgccgtcg cagatggcat ccacgtcaac ttctactgcg tcatcaagga aatgatccag
    43921 caacactggc gcatcgttag acacgctaac cgccgtctgg aagtagcgac gcaggtcagc
    43981 ttcgtcatag acgatttcca tcgcccgacc gccgagaacg taagacggac gcaccaccag
    44041 cgggtagcca atctcttttg ccttctcaac tgccatttca atagcagtaa cggtggcgtt
    44101 cgccggttgt ttcagtttca gacggtcaac cgcatgctgg aagcgttcac ggtcttccgc
    44161 acggtcgata gcatccgggc tggtgccgat aaccggtacg ccagcggctt ccagtgcacg
    44221 cgccagtttc agcggggtct gaccgccgta ctgaacgata acgcctttcg gcttctcaat
    44281 acgcacgatt tccagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt
    44341 gtcgtagtcg gtagagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc
    44401 ttcacgcagc gccagcgagg cgtgtacgca gcagtagtcg aattcgatac cctgaccgat
    44461 acggtttgga ccgccgccaa gcaccatgat tttttcacgg tcggtagacg gattcgcttc
    44521 gcactcttct tcataagtgg agtacatgta agcggtgtcg gtggcaaact ctgccgcaca
    44581 ggtatccacg cgcttgtaga ccgggtgcag gtcatattgg tcacgcagct tacggatttc
    44641 cgcttcgcgt acgcccgcca gttttgccaa gcgcgcatcg gcaaagcctt tgcgtttcag
    44701 ctggcgcagg aattcagcgt tcaggccagt gatgcccact tccgccactt tctcttccag
    44761 acgcaccagt tcttcaatct gtaccaggaa ccagcggtca atgttagtca ggttgaagac
    44821 gccgtccaca gacaggcccg cgcggaaagc atcggcgatg taccagatac gctctgcgcc
    44881 tgcgtctttc agttcgcgac ggattttggt taacgcttcc gggtcatcca ggctcacttt
    44941 cgggtcgaat ccagtcgcac cgacttccag gccgcgcagc gctttttgca gggattcctg
    45001 ctgcgtgcga ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc
    45061 gttagcaccg gcgaattttt cgaagttgaa gcgaggaatt ttggtaacca cgtaatcgat
    45121 ggacggctcg aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcatcgag
    45181 ggtgtaaccc accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga
    45241 cgccagcgcc gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt
    45301 tttcgggttc accgcaaact ggacattgga accaccggtt tcaacgccga tttcacgcag
    45361 caccgccatc gaggcgttac gcatgatttg atattctttg tcggtcagcg tttgggctgg
    45421 cgcgacagtg atggagtcac cggtgtggat gcccatcgca tcaaagtttt cgatagagca
    45481 gacgatgatg cagttgtcgt ttttatcgcg caccacttcc atctcgtact ctttccagcc
    45541 gatcagcgac tcatcaatca gcaactcttt ggtcggagag agatccagac cgcgggcgca
    45601 aatttcttca aactcttcgc ggttataagc gataccgccg ccgctaccgc ccatggtaaa
    45661 ggatgggcga ataatgcacg ggaagcccac gtcagcggca accgccagcg cttcttccat
    45721 cgtatgtgcg ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa
    45781 acgacggcgg tcttctgctt tatcaatcgc atcggcagtg gcaccaatca tggtgacgcc
    45841 gaactcttcc aacacgccct gacgttccag ctccagcgcg cagttcagcg ccgtctgacc
    45901 gcccatggtt ggcagcaccg cgtccgggcg ctctttttca ataatcttac gtaccacttc
    45961 ccagtgaatc ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc
    46021 cgggttggag ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg
    46081 cgcgccagag tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat
    46141 caggatactt tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg
    46201 gttttacggt actgctcaat taactcgata aagtggtcga acaacggcgc ggcgtcgtgt
    46261 ggaccagggc tggcttcagg gtgaccctgg aagctgaacg ccggtttatc ggtgcgatga
    46321 atgccctgta acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat
    46381 gttgcttcat ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc
    46441 acatctttaa ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca
    46501 ccgctcgcca gcgccagcag ctgatgaccg aggcagatgc cgaagaccgg aatatcggtt
    46561 tcgaggaatt tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg
    46621 ttggagagga agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc
    46681 ggaacgatgg tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgtttggca
    46741 ccaaaatcat aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg
    46801 ccgccggtca acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct
    46861 tttgccagat ccatgccatt cagacctggg aacgcgcggg ctttttctaa cgccagcgcc
    46921 gcatccgggt tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa
    46981 cgcgtcagct tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa
    47041 gaagagaggt cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc
    47101 agaccttgtg catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca
    47161 atatggggat aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct
    47221 tgataaccgg tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct
    47281 atggcccgac cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa
    47341 acaccctcca gagaatattc actcacttta tttgcatatt aattcacaat gatgacgtaa
    47401 atcaatgcaa atctgcttac cgacgaattt ctggcaaacg gcggcattct ggagatatgg
    47461 gcgctaaaag tcaacttaat ggcaggattt tttatattat tttgtggctc atgcatgttt
    47521 tacaggcatt aagcgacaaa aagatcatct aacctgttct ggaaaacgct tgcgcagcaa
    47581 gaagattgca tgaaataaaa acgagcaggc aaaagtggac caaatggtca aaatttacat
    47641 taagacaaca aaaatag
//
LOCUS       DAZFBR010000024        45118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFBR010000024 DAZFBR010000000
VERSION     DAZFBR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.024"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..1094)
                     /gene="espY3"
                     /locus_tag="ACHJZ2_001541"
     CDS             complement(<1..1094)
                     /gene="espY3"
                     /locus_tag="ACHJZ2_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HFO4567684.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDRLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKDGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNA
                     NCTNANFNNCRFKKCDLTNMNCTGAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKS
                     DGNETKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYR
                     TDLFNPILKTEAESTEEKDISPLAKIILDY"
     gene            complement(1325..2299)
                     /gene="yajO"
                     /locus_tag="ACHJZ2_001542"
     CDS             complement(1325..2299)
                     /gene="yajO"
                     /locus_tag="ACHJZ2_001542"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO4567685.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(2354..4216)
                     /gene="dxs"
                     /locus_tag="ACHJZ2_001543"
     CDS             complement(2354..4216)
                     /gene="dxs"
                     /locus_tag="ACHJZ2_001543"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO4567686.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(4241..5140)
                     /gene="ispA"
                     /locus_tag="ACHJZ2_001544"
     CDS             complement(4241..5140)
                     /gene="ispA"
                     /locus_tag="ACHJZ2_001544"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO4567687.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            complement(5140..5382)
                     /gene="xseB"
                     /locus_tag="ACHJZ2_001545"
     CDS             complement(5140..5382)
                     /gene="xseB"
                     /locus_tag="ACHJZ2_001545"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO4567688.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5588..7036
                     /gene="thiI"
                     /locus_tag="ACHJZ2_001546"
     CDS             5588..7036
                     /gene="thiI"
                     /locus_tag="ACHJZ2_001546"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO4567689.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            complement(7090..7680)
                     /gene="yajL"
                     /locus_tag="ACHJZ2_001547"
     CDS             complement(7090..7680)
                     /gene="yajL"
                     /locus_tag="ACHJZ2_001547"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO4567690.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(7643..8554)
                     /gene="panE"
                     /locus_tag="ACHJZ2_001548"
     CDS             complement(7643..8554)
                     /gene="panE"
                     /locus_tag="ACHJZ2_001548"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO4567691.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8722..9213
                     /gene="yajQ"
                     /locus_tag="ACHJZ2_001549"
     CDS             8722..9213
                     /gene="yajQ"
                     /locus_tag="ACHJZ2_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO4567692.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(9341..10705)
                     /gene="yajR"
                     /locus_tag="ACHJZ2_001550"
                     /pseudo
     CDS             complement(9341..10705)
                     /gene="yajR"
                     /locus_tag="ACHJZ2_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10854..11744)
                     /gene="cyoE"
                     /locus_tag="ACHJZ2_001551"
     CDS             complement(10854..11744)
                     /gene="cyoE"
                     /locus_tag="ACHJZ2_001551"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO4567693.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(11756..12085)
                     /gene="cyoD"
                     /locus_tag="ACHJZ2_001552"
     CDS             complement(11756..12085)
                     /gene="cyoD"
                     /locus_tag="ACHJZ2_001552"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO4567694.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(12085..12699)
                     /gene="cyoC"
                     /locus_tag="ACHJZ2_001553"
     CDS             complement(12085..12699)
                     /gene="cyoC"
                     /locus_tag="ACHJZ2_001553"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO4567695.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(12689..14680)
                     /gene="cyoB"
                     /locus_tag="ACHJZ2_001554"
     CDS             complement(12689..14680)
                     /gene="cyoB"
                     /locus_tag="ACHJZ2_001554"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO4567696.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(14702..15649)
                     /gene="cyoA"
                     /locus_tag="ACHJZ2_001555"
     CDS             complement(14702..15649)
                     /gene="cyoA"
                     /locus_tag="ACHJZ2_001555"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO4567697.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(16109..17584)
                     /gene="ampG"
                     /locus_tag="ACHJZ2_001556"
     CDS             complement(16109..17584)
                     /gene="ampG"
                     /locus_tag="ACHJZ2_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO4567698.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(17628..18206)
                     /gene="yajG"
                     /locus_tag="ACHJZ2_001557"
     CDS             complement(17628..18206)
                     /gene="yajG"
                     /locus_tag="ACHJZ2_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4567699.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            18511..18828
                     /gene="bolA"
                     /locus_tag="ACHJZ2_001558"
     CDS             18511..18828
                     /gene="bolA"
                     /locus_tag="ACHJZ2_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO4567700.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            19172..20470
                     /gene="tig"
                     /locus_tag="ACHJZ2_001559"
     CDS             19172..20470
                     /gene="tig"
                     /locus_tag="ACHJZ2_001559"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO4567701.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            20715..21338
                     /gene="clpP"
                     /locus_tag="ACHJZ2_001560"
     CDS             20715..21338
                     /gene="clpP"
                     /locus_tag="ACHJZ2_001560"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO4567702.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            21464..22738
                     /gene="clpX"
                     /locus_tag="ACHJZ2_001561"
     CDS             21464..22738
                     /gene="clpX"
                     /locus_tag="ACHJZ2_001561"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO4567703.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            22926..25280
                     /gene="lon"
                     /locus_tag="ACHJZ2_001562"
     CDS             22926..25280
                     /gene="lon"
                     /locus_tag="ACHJZ2_001562"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO4567704.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            25471..25761
                     /gene="hupB"
                     /locus_tag="ACHJZ2_001563"
     CDS             25471..25761
                     /gene="hupB"
                     /locus_tag="ACHJZ2_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO4567705.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            25953..27824
                     /gene="ppiD"
                     /locus_tag="ACHJZ2_001564"
     CDS             25953..27824
                     /gene="ppiD"
                     /locus_tag="ACHJZ2_001564"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4567706.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            27975..28346
                     /gene="ybaV"
                     /locus_tag="ACHJZ2_001565"
     CDS             27975..28346
                     /gene="ybaV"
                     /locus_tag="ACHJZ2_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO4567707.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            28452..28850
                     /gene="fadM"
                     /locus_tag="ACHJZ2_001566"
     CDS             28452..28850
                     /gene="fadM"
                     /locus_tag="ACHJZ2_001566"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO4567708.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(28902..29597)
                     /gene="queC"
                     /locus_tag="ACHJZ2_001567"
     CDS             complement(28902..29597)
                     /gene="queC"
                     /locus_tag="ACHJZ2_001567"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO4567709.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(29662..31362)
                     /gene="ybaE"
                     /locus_tag="ACHJZ2_001568"
     CDS             complement(29662..31362)
                     /gene="ybaE"
                     /locus_tag="ACHJZ2_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO4567710.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            31462..32280
                     /gene="cof"
                     /locus_tag="ACHJZ2_001569"
     CDS             31462..32280
                     /gene="cof"
                     /locus_tag="ACHJZ2_001569"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO4567711.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            32432..32890
                     /gene="decR"
                     /locus_tag="ACHJZ2_001570"
     CDS             32432..32890
                     /gene="decR"
                     /locus_tag="ACHJZ2_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO4567712.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            32920..34692
                     /gene="mdlA"
                     /locus_tag="ACHJZ2_001571"
     CDS             32920..34692
                     /gene="mdlA"
                     /locus_tag="ACHJZ2_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4567713.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            34685..36466
                     /gene="mdlB"
                     /locus_tag="ACHJZ2_001572"
     CDS             34685..36466
                     /gene="mdlB"
                     /locus_tag="ACHJZ2_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4567714.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            36647..36985
                     /gene="glnK"
                     /locus_tag="ACHJZ2_001573"
     CDS             36647..36985
                     /gene="glnK"
                     /locus_tag="ACHJZ2_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO4567715.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            37015..38301
                     /gene="amtB"
                     /locus_tag="ACHJZ2_001574"
     CDS             37015..38301
                     /gene="amtB"
                     /locus_tag="ACHJZ2_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO4567716.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(38350..39210)
                     /gene="tesB"
                     /locus_tag="ACHJZ2_001575"
     CDS             complement(38350..39210)
                     /gene="tesB"
                     /locus_tag="ACHJZ2_001575"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO4567717.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            39428..40000
                     /gene="ybaY"
                     /locus_tag="ACHJZ2_001576"
     CDS             39428..40000
                     /gene="ybaY"
                     /locus_tag="ACHJZ2_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO4567718.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(40033..40344)
                     /locus_tag="ACHJZ2_001577"
     CDS             complement(40033..40344)
                     /locus_tag="ACHJZ2_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO4567719.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            40507..40603
                     /gene="ffs"
                     /locus_tag="ACHJZ2_001578"
     ncRNA           40507..40603
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHJZ2_001578"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            40723..41076
                     /gene="ybaA"
                     /locus_tag="ACHJZ2_001579"
     CDS             40723..41076
                     /gene="ybaA"
                     /locus_tag="ACHJZ2_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO4567720.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(41118..42668)
                     /gene="pdeB"
                     /locus_tag="ACHJZ2_001580"
     CDS             complement(41118..42668)
                     /gene="pdeB"
                     /locus_tag="ACHJZ2_001580"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO4567721.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(42832..43302)
                     /gene="ylaC"
                     /locus_tag="ACHJZ2_001581"
     CDS             complement(42832..43302)
                     /gene="ylaC"
                     /locus_tag="ACHJZ2_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO4567722.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(43418..43969)
                     /gene="maa"
                     /locus_tag="ACHJZ2_001582"
     CDS             complement(43418..43969)
                     /gene="maa"
                     /locus_tag="ACHJZ2_001582"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO4567723.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(44141..44359)
                     /gene="hha"
                     /locus_tag="ACHJZ2_001583"
     CDS             complement(44141..44359)
                     /gene="hha"
                     /locus_tag="ACHJZ2_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO4567724.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(44385..44759)
                     /gene="tomB"
                     /locus_tag="ACHJZ2_001584"
     CDS             complement(44385..44759)
                     /gene="tomB"
                     /locus_tag="ACHJZ2_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO4567725.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        11365 a        11086 c        11862 g        10805 t
ORIGIN      
        1 atatagtcaa gaataatttt tgctaatggg ctaatgtctt tttcttctgt tgattctgct
       61 tctgttttta atatcggatt aaaaaggtcg gtacggtaaa gtttcgtgcc tttcatcctg
      121 acgatgtcag gaagaatagt gtttgaaaga ttagcctcgc taaagtccac attttggcaa
      181 tcactttctg ataaatctgc ccaattgaaa gtggttttca cacaggagca gttagttaga
      241 atcatccctt tcgtttcatt cccatctgat ttctgataag ttatcctttg tattatttga
      301 tctgcttctg gatattgcat ctcaggttct ttttctttac catatatcat cgcattatca
      361 agaattgcgc cagtgcaatt catatttgtc aggtcgcatt tcttaaatct acaattgtta
      421 aaattcgcat ttgtacaatt cgcatttgta aacttgacgc cataaaaata agtatgttcg
      481 aagttgacat ttgagcagtt acagtttccc atatctatac cgaaatctat atcgggatct
      541 ttattgggat ctttaccgga aatattgctg tgtgctagat atagaccgct gaaatcgtaa
      601 tatccattgt catccttttc tattgggagt gaataagtct ttactacctc gttaacaata
      661 gagatagttc cacgtatcat cagattttct ccgtctttat aaaaaataat gttatgtttg
      721 atggtactgt tgtatggctc tggcaatgag gatgcaattt tattccagaa acaacaaagc
      781 gtggtctgtt tttgtttggg cgagaggcga tcaaaatcca gctttaaata tgagtctaaa
      841 tctgacgttg catctatatt aaagttggga tctggagtta acgctgaatt ataatttaga
      901 actgacaatg tattgatgta tttatgaatc gatttaaatg ccttttgttt atcactgtcc
      961 agaaaaaaat cacgaatttt gttccacagc ctttcaataa gattttgatt taaatcttgc
     1021 ttcattgagt ttcttaaatc tgcaaccggt acatcagtaa acaggatcgg ttgtttggtg
     1081 atggttttca tcatgattgt aatcctctta cgttgactaa ataagacagt tttaggggat
     1141 attacctgga gaaactatca aaaatcgctc aaagggatga agacgatgaa tagctgcaaa
     1201 ctcattcacg aagaatttct cttgcagagg tggattatgg ttacagaagg tgatgggagg
     1261 tacgttgtcc ccatcatcgc agacgatggg gaattcagca gataaaatgg gtgaaaagcg
     1321 aaatttactt aaatcctacg accggatgcg gtttatacgg tgtttccagt tcagcaatct
     1381 gttccggctt caaagtgata tccaccgcgt tcagcagctc atcaagctgt tcttcccgcg
     1441 aagttccgat aatcggtgcg gcaatgcccg gtttactcaa caaccaggcc agcgcaactt
     1501 gtgctcgtgt tgcaccgagt tcttcactga cgcccgttaa ccgctctgcg atctgcgcgt
     1561 cattttcatc gctttcttta tagagatttt tccccacctc atcagacacc agtcgtgcgg
     1621 tagtttctcc ccacggacgc gtcagtcgcc cccgcgccag cggactccac ggaatgaccg
     1681 ccacgccttc ctgataacac agcggcagca tctcgcgctc ttcttcgcga taaatcagat
     1741 tgtagtgatc ctgcatactg acaaactgcg cccagccgtg ctgtttttgt agttccagtg
     1801 cctgagcaaa ctgcgaagcg tgcatggatg acgcgccgat ataacgcgct ttcccggctt
     1861 ttaccacgtc gttgagggct tccagcgtct cttcgatcgg cgtgttgtaa tcccagcgat
     1921 gaatttgcag gatatcgaca taatccatgc cgagacgtcg caggctgtcg tcgatagagc
     1981 gcaaaatttg tgcacgggat aatccttccg gtaaatcacc aacgcgatgg aacactttgg
     2041 tggcaacgac cacgtcttca cgacgggaga aatcccgcag tgcgcgaccg acgatctctt
     2101 cgctgctgcc atcggaatag ctattggcgg tatcaaagaa atttatgccg ccttccagtg
     2161 cgcgtttaat gatgggacgg ctgctctctt ccggcagtgt ccatgcgtga ttaccacgat
     2221 ctggctcgcc aaaggtcata cagccgaggc aaagtcggga aacgcgaagg tcggtttttc
     2281 ctaaggggtt gtattgcatg ctgccactcc tgccatgaat aatttcttaa gcatagcagg
     2341 agtggagtag ggattatgcc agccaggcct tgattttggc ttccataccg gcggcatcga
     2401 ggccgagttc ggcgcgcatt tcttcctgag ttccttgcgg aataaagaag tccggcaggc
     2461 caatgttcag cacgggtact ggtttacgat gggccatcag cacttcgttc actccgctgc
     2521 ctgcaccgcc cataatggcg ttttcttcta cggtaaccag tgcttcatga ctggcggcca
     2581 tttccagaat taacgtttca tcaagcggtt tcacaaaacg catatcgacc agcgtggcgt
     2641 tcagcgattc ggcgactttc gccgcttctg gcatcagcgt accaaagtta aggatcgcca
     2701 gtttctcgcc acgacgcttc acaatgcctt tgccaattgg tagtttttcc agcggcgtca
     2761 gttccacgcc gaccgcgttg ccacgcgggt agcgcaccgc tgacgggcca tcgttatagt
     2821 gatagccggt atagagcatc tggcgacatt cgttttcatc gctcggggtc ataatgacca
     2881 tttccggtat gcagcgcagg taagagagat caaaagcgcc ctggtgggtt tgaccgtcag
     2941 caccaacaat gcccgcgcgg tcgatggcga acaggaccgg gagtttttga attgccacgt
     3001 catgcagcac ctgatcatag gcgcgttgca ggaaggtgga gtaaattgcc acgatcggtt
     3061 tgtagccacc aatcgccaga ccggcggcaa aggtcaccgc gtgttgctcg gcaatcgcta
     3121 catcaaaata gcgatccggg aatttacgtg aaaactcgac catgccggaa ccttcacgca
     3181 tcgccggagt aatcgccatc agtttgttat ctttcgctgc cgtttcgcac aaccagtcgc
     3241 caaagatttt tgaatagctt ggtaaaccac cgctactttt cggcaaacaa ccgctggagg
     3301 gatcaaattt aggcacggcg tggaaggtga ttgggtcttt ttctgccggt tcataaccac
     3361 gacctttttt ggtcatgata tgcaggaact gcgggccttt caggtcgcgc atgttcttca
     3421 gcgtggtgat aagccccagc acatcgtgac cgtcaaccgg gccgatgtag ttaaagccca
     3481 gctcttcaaa caacgtgcca ggcaccacca tgcctttaat atgttcttcg gtacgtttga
     3541 gcagctcttt aattggcgga acgccagaga aaactttttt cccgccttcg cgcagtgaag
     3601 agtaaagctt accggaaagc agctgcgcca gatggttgtt aagcgcgcca acattttcgg
     3661 aaatcgacat ttcattgtcg ttgaggacca ccagcatatc aggacgaata tcgcccgcgt
     3721 gattcatcgc ttcaaacgcc atgcctgcgg taatcgcgcc gtcgccaatc acacagacgg
     3781 tgcggcgatt tttgccttct ttctcggcag caaccgcaat accaattccg gcactgatgg
     3841 aggttgatga atgcccgacg cttaatacgt catattcgct ttcgccgcgc cacgggaacg
     3901 ggtgtagacc gcctttctga cggatggtgc cgattttgtc gcggcgtccg gtcaaaattt
     3961 tatgcggata agcctgatgc cccacatccc aaatcaattg gtcaaacggg gtgttataga
     4021 catagtgcag cgccacggtc agttcgaccg tgcccagccc ggaggcgaag tgcccgctgg
     4081 aacggctcac gctgtcgagt aaatagcggc gcagttcgtc gcagagtttc ggtaaactct
     4141 cttttggcaa cagtcgtagc tcctgggtgg agtcgaccag tgccagggtc gggtatttgg
     4201 caatatcaaa actcatcagg ggcctattaa cacttattgt ttatttatta cgctggatga
     4261 tgtagtccgc tagcgcttcc agtgccgaag tatcgatcga ctgttcagcc agttgtttca
     4321 gcgactggcg ggcatcgtcg atcagatcct gggctttctt ccgggcttgc tcaagaccca
     4381 ggagtgcagg gtaggtactt ttgccaagct gctggtcggc accctgacgt tttcccaacg
     4441 ttgcagtatc tcccaccaca tccaggatgt catcctgaac ctggaaggca aggccgatgc
     4501 tctctgcgta cttgtcgagt actggtagag cacgacgtcc tttatctccg gcgcttaatg
     4561 caccaaggcg aacggcggcg cgaattaatg cgcctgtttt atgacgatga atacgctcaa
     4621 gcgcgtccag aggtacgtgt ttgccttccg cgtctaaatc caacgcctga ccaccacaca
     4681 ttccggcaat accgctggcg ctcgccagtt cagaaatcat cgaaattctg tcgcgatccg
     4741 acacttctgg catatcagca tcgcttaaaa tcgagaacgc cagcgtttgt aaagcgtcgc
     4801 cagcgagaat cgcgttcgct tcgccaaact tcacatggca ggtcggcaag ccgcgacgca
     4861 gatcgtcatc atccatcgcc ggtaaatcat catgaattaa tgagtaagcg tggatacact
     4921 ctacggcggc agcgggtgcg tccagcgtgt ttgtgctaac gccgaacata tgaccggtgg
     4981 cataaaccag gaaaggtcgc aggcgcttac cacctaataa tgcgccatac tgcatggttt
     5041 cgaccacggg agtgttctga aagggcagtg gggcgataaa acggctcagc gcctggttgg
     5101 cctgcttaac gcaggcttcg agttgctgcg gaaagtccat tactcattgt ccggtgtaaa
     5161 aggggttaga gaggcgtctt cgttgtcaga cagcagaatt tgtacgcgct gttcggcttg
     5221 ttgtaatttg gcctgcccct gacgtgccag ctgcacgccg cgttcgaact cgttcagcgc
     5281 ctcttccagc ggcaggtcgc cactttccag acgggttaca atctgttcca gctcgctcag
     5341 cgccttttca aagctggcgg gcgcctcatt tttcttcggc ataatgaatg tctgactctc
     5401 aatatttttc gccccgtcat ggtaacggac tcagggcaaa tagcaaataa cgcgcaatgg
     5461 taaggtgatg tgcgcagcaa agcgatgtta gtggtatact tccgcgcctg gatgcagccg
     5521 caggtgtggg ctgctgtatt tttccctata caagtcgctt aaggcttgcc aacgaaccat
     5581 tgccgccatg aagtttatca ttaaattgtt cccggaaatc accatcaaaa gccaatctgt
     5641 gcgcttgcgc tttataaaaa tccttaccgg gaacattcgt aacgttttaa agcactatga
     5701 tgagacgctc gctgtcgtcc gccactggga taacatcgaa gttcgcgcga aagatgaaaa
     5761 ccagcgtctg actattcgcg acgccctgac ccgtattcct ggtatccacc atattctcga
     5821 agtcgaagac gtgccgttta ccgacatgca cgatattttc gagaaagcgt tggttcagta
     5881 tcgcgatcag ctggatggca aaaccttctg cgtacgcgtg aagcgccgtg gcaaacatga
     5941 ttttagctcg attgatgtgg aacgttacgt cggcggcggt ttaaatcagc atattgaatc
     6001 cgcgcgcgtg aagctgacca atccggatgt gactgtccat ctggaagtgg aagacgatcg
     6061 tctcctgctg attaaaggcc gctacgaagg tattggcggt ttcccgatcg gcacccagga
     6121 agatgtgctg tcgctcattt ccggtggttt cgactccggt gtttccagtt atatgttgat
     6181 gcgtcgcggc tgccgcgtgc attactgctt ctttaacctc ggcggcgcag cgcatgaaat
     6241 tggcgtgcgt caggtggcac attatctgtg gaaccgcttt ggcagctccc accgcgtgcg
     6301 ttttgtcgct attaatttcg agccggtagt cggggaaatt ctcgagaaaa tcgacgacgg
     6361 tcagatgggc gttatcctca aacgtatgat ggtgcgtgcc gcgtctaaag tggctgaacg
     6421 ttacggcgta caggcgctgg tcaccggcga agcgctcggc caggtgtcca gtcagacgct
     6481 gaccaacctg cgcctgattg ataacgtctc tgatacgctg atcctgcgtc cgctgatctc
     6541 ttacgacaaa gagcacatca tcaacctggc tcgccagatt ggcaccgaag actttgcccg
     6601 tactatgccg gaatactgcg gtgtgatctc caaaagcccg acggtgaaag cggttaagtc
     6661 gaagattgaa gcggaagaag agaaattcga cttcagcatt ctcgataaag tggttgaaga
     6721 agcgaataac gttgatatcc gcgaaatcgc ccagcagacc gggcaggaag tggtggaagt
     6781 ggaaaccgtc aatgacttcg gcccgaacga cgtgattctc gatatccgtt ctgtcgatga
     6841 acaggaagat aagccactga aagtcgaagg gattgatgtg gtttcactgc cgttctacaa
     6901 gttgagcacc aaatttggcg atctcgacca gaacagaacc tggctactgt ggtgtgagcg
     6961 cggggtaatg agccgcctgc aggcgctcta tctacgcgaa cagggcttta agaatgtgaa
     7021 ggtgtatcgt ccgtaatttg tggtttttac gtcgcatctg gtcagatgcg acgtttgccg
     7081 catccgacac tactcgtaat aattataaat ccctgccgcc atcaccagtt gtgatgccac
     7141 ttcatgggct ttctcgcgcc caaccagcag gtcgataatt ttcagaccaa agtcgatagc
     7201 tgtacccggc ccctggctgg tcagcaattt tacccgtgca tcccagacga cgcgcttgtc
     7261 ctgccattgt tcagcgggaa ttttgtcttt cagcgtcggg aagccggtca tattgccaat
     7321 cggaaagata tcgtgcggca ccagcacggt ggctggcgcg gcgcaaatag ccgcgacgat
     7381 acgcccggaa cggtggaact gtttaacggt ttcaaccagc agggtactat cgcgaaaaca
     7441 ctccgcgcct ttaatgccac caggcagcac gatcacgtca tattcgccat cagccacttc
     7501 gaccagcggc gcatccgcca gcagcttcac gccgcgcgag caggtaatcg ccaggttgcc
     7561 atcgctggct acgctggcag tggtgacttt gataccgccg cgaatcaaga ggtcgatcgt
     7621 ggtgacggct tcagtctctt cactaccagg ggcgaggcaa accagtgccg atgcgctcat
     7681 attcactctc ctttcttttt accatttcaa acaggcgggt gttttccggt acggcaatcc
     7741 catgcgcgcg ggcgcggcgt aagagaaaac cattgatata gtcgatttcg gtgtggcgca
     7801 gcgtgcggat atcctgcaac atcgacgaga tattttccgc tgtggcatca atcacctgca
     7861 tcacgtaatc acgtaaatct tccgctgaag tatgatgccc ttcgcgttcg atcactgccg
     7921 cgacttcttc gcatatttgc ataatttctt gcggatgatg acgtaattca ccgttcgggc
     7981 aattccagat ggcagtcagt ggattaatca cacagttgac tgccagcttg cgccacagct
     8041 cggcgcgaat attgttatgc caggcaacgt caggcaacac ggtttgcaat atatccgcca
     8101 gataactgta atcgccgtcc tgttgccgtg ctggaccaat atgcgtgata ccgtttgcca
     8161 catgaataat gacattgccg tcgcggcagg cggcatgggt ggtggtgccc atcagtaatg
     8221 gctgctgaat gttttgcaat tcttcgatgg tgcccatgcc gttgtgaatt aacagtattg
     8281 gcgtagttac aggcagtgtg gacgcgaggc ttttgacggc atcggaaacc tgccatgctt
     8341 tcagcgtcac caggagcaga tcgctggtgg caagaaaatc gggatcgttg gcggtcagcg
     8401 attcgttaaa tatcgaacca tcagtctcaa ccagattcac actacaataa ggttgcggta
     8461 cgcgcagcca gccctgaact tcgtgaccct gtttacaaag tgctgtaagc cataattgcc
     8521 ctaaggcacc gcatcccaat acggtaattt tcattgttcc tcctcacccg caaccactcc
     8581 gggtgttcaa taaggctatc ccttaattgt gcatgctgtt gcgactatgc acaattaagg
     8641 gatacgtcct ggtgcaggac tgttggttat ttaactttgc aggtattatg cttcgcatca
     8701 aaaatgaagg gagaggaaaa gatgccatct ttcgatattg tctctgaagt tgatcttcag
     8761 gaagcacgta acgcggtcga taacgcgagc cgcgaagtgg agtcccgttt tgacttccgt
     8821 aacgttgaag cctcatttga gctgaacgac gccagcaaaa ccatcaaagt gttgagcgag
     8881 tccgacttcc aggtcaatca gttgctggat attctgcgtg ccaagctgct gaagcgcggc
     8941 attgaaggca gttcactgga tgtaccggaa aatatcgttc atagcggtaa aacctggttt
     9001 gtggaagcga aactgaaaca gggcattgag agcgccactc agaaaaaaat cgtcaagatg
     9061 atcaaagaca gcaaactgaa agtgcaggcg caaattcagg gcgatgagat ccgtgtgacg
     9121 ggcaaatctc gtgatgattt acaggctgtc atggcgatgg tgcgcggtgg cgatctcggt
     9181 cagccgttcc agttcaaaaa cttccgcgat taatcgcgac aacctgcgct ttgttcatgt
     9241 cagatgcggc atgaacgcct ggttcggcct acaaagtttt gtaaatcagg aacttagtag
     9301 gcctgataag cgtagcgcag caggcaattt cgcctcgctt ttatgcctgg cgaattgcct
     9361 gttcaacatc aaagcgattc gtcactttgc tgtcgatttt cacataagct gaatgttctt
     9421 cttctgcaat caacacttct ttgatgcctt cagtttccag caaacgcact tttaacgcct
     9481 cgtttgcggc aatgttcgcc gggatttcaa tgcgcaaact gctgacatac ggcggttctt
     9541 tcatggtact ggcgactgcc agtcacactg cggccagcat tgcgccagcg agaaataccc
     9601 cctgaccgtc aaacatgccg tcaatccagc cgcccagcga accgccaatc gccacgccaa
     9661 gaaactggct ggtggagtaa acacccatcg ccgtaccttt gtaacctgct ggcgactctt
     9721 tactgataag tgagggcagg agggcttcca tcaaattaaa cgccacaaag aaaagctgca
     9781 cgccgaccac cagttgccag aactgcgttt gcgcgttcct caacacaatt tccgcaacca
     9841 cgatcaaccc gacgcagaag acaaagactt gcttcatttt gcgcttaact tcagcgtaga
     9901 taatgaaagg cacgaccgag ccaaaggcga ttagcattgt cgccagatag accttccagt
     9961 gttcagccgc cgggaacccc gcatcagcca gttgtccggg cagggcaaca aacgtcgaca
    10021 tcagcaaaat atgcagacac ataatgccaa agttgagttt cagcagccgc ggttccgcca
    10081 gcaccttact gaaactgcct ttcaccattc cggactcacg attaagtacg tgagtgctac
    10141 tgttgggcac aacccaaatg gtcaacgcaa tgccggtcgt tgccagaata gcgatcatcc
    10201 agaacagcgc gtgcagccca agtttgtgag tgatgatcgg gccaagcacc atcgcaatgg
    10261 caaaggtaat gccaaagctc acgccgataa acgccattgc tttggtgcgg ttttgttcgc
    10321 gcgtgagatc ggaaagcagc gccataacgg cagcggcaat cgcaccggag ccttgtagcg
    10381 cccgacccag aataattccc cagatggagt cagagagcgc agcgataacg ctaccggcgg
    10441 caaacactgc cagcccaccg acaattaatg gtttgcgacc aatgcggtcg gaaagcaggc
    10501 caaacggaat ctgaaaaacg gcctgagtca gaccataaat accaatggca ataccgatta
    10561 atgcttcgct ggcaccttgc agagccatgc cgtacgtggt cagaaccggc agaaccatga
    10621 acatgcccag catgcgcaac gagaatacgg tccctaaacc ccaggtcgcg cgccgctcac
    10681 ctggcgtcat tttataatcg ttcattacca cctctgtttt aaattcgcga ctagtgtaaa
    10741 gcggcaaacg aatggggtaa acatgcggat tttttagtaa atatttcgag agggatatgt
    10801 ttctaatgct aagaaaaaag gtgccgtagc acctttttaa tagagaggtt gtgttaccac
    10861 acagcagcca gcagcgtatg cgagtccggc accataaaat caacggacat catcaccgag
    10921 agggcagtga tggcgatgat agagaagccg aacagcttgc gcgcccagat tctgtcatca
    10981 gcaactttat aaccgcgcag agccatacct aaccaccaga cgctaaccgc cgcggcgacc
    11041 accagatatt tatatccagc gtaaccgcca agagagagca tcagcgtggc aacggcaaag
    11101 gcgatgatat acagcgtgat gtgattcttc gccaccgaaa tgccttttac cactggcaat
    11161 accggaatgt ttgccgcctg gtaatcctta aagcggaaaa tggcgatggc ataggagtga
    11221 ggcatctgcc acaggctgaa gatagccagc aggatcgctg cgccgctatc gaactcgccg
    11281 gttactgcac agtagccaat caccggcggc gcagcgccgg agagcgaacc aatcaatgtg
    11341 ccgtagacgg agtggcgttt catgtacagg ctataaacgc cgacataaac cacaaagccc
    11401 atcaccccca gccagcaggc cagcggattc gcgccaaacc acagcagcat aaagccagca
    11461 atacccagca aggtggcgta caccagcgag acagcaggag agatcaggcc tttaaccagc
    11521 acccgattct tcgtcctttc catctttctg tcgatatccc tgtcgatgta gttgttaaac
    11581 acacaacccg acgccacaac cagtgacacc ccaaccagcg tgtagataaa caggggataa
    11641 tcaatgctgc cctttgaggc cagcaggaat cccccaatca ccgagatcag gttgccaaag
    11701 atgatgcctg gtttcgttac ttgcaggtat tgcttaaaca tcataaccgc cgctcttagt
    11761 gcatcatcat gttgtagttg aggttccaca taatccagat ggagcctaca actaggatag
    11821 cgatgattag cacggtgaag acaaacgccg tcatgttcca gccttcatct gatttggtat
    11881 tcatgtgcag gaagcacacc agatgcacca gaacctgtac cactgccatt gccaggattg
    11941 ttcccagaat tacggccgga gaggcagccc ctgtcatcac catccagaac ggaatcaccg
    12001 tcagaatgat cgacaggata aagcctgtca tgtaggtttt tacgctgcca tgggacgcgc
    12061 cgctgtgatc ggtagaatga ctcattacat cgcccccatc agataaacaa cagtgaacac
    12121 acagatccaa accacatcca ggaagtgcca gaacaggctc aggcacatga tgcgggtacg
    12181 gttagtgctg gtcaggccgc gacgagcgat ttgtaccatc agcaccgcca tccagataag
    12241 accggaagtg acgtgcagac cgtgcgtgcc gaccagcgca aagaacgctg acaggaagcc
    12301 gctgcgatcc ggacccatgc cgttaacaat caggtgatgg aattcgtaga tttccatccc
    12361 gataaatccg gcgccaaaca accaggtcaa cgccagccag gagataacct ggcttttgtt
    12421 gtttttgtac atggcgatag ccgccatgcc gtaggtgatg gagctgaaca acagcaagaa
    12481 agtttcaacc agaacgaacg gcagttcgaa aatgtcctta cctgtcgggc cgcctgcggt
    12541 gccgttcacc agaacggcat aggtagcaaa caagatagag aacagaatgc agtcgctcat
    12601 caggtagatc caaaatccga agattttggt tccgcctgca tcgtggtgcc cgtgttcgtg
    12661 cgcgtggaca gtcgcgtgcg tcaaagtatc agttgccatt tttcagccct gccttagtaa
    12721 tctcatcgaa atgctggttt tccagttttt cgatttctgc caccggcacg tagtaatcca
    12781 cgtcctcgtc gaagcttttc acgatccagg tgatgatcat gcctgcgaag ccaacaatcg
    12841 ccagccacca gatatgccag atcatggcga aaccgaagat ggtggagaaa gccgcaatga
    12901 cgatacctgc accgctgttt ttcggcatat gaatttcttc atagtggtca ggctttttat
    12961 acgcttcgcc tttctctttc atttcccaga atgcatcacg ctcgtgaacg tgcggcacaa
    13021 cggcaaagtt atagaacgga ggcggggaag aggttgccca ctccagcgta cggccacccc
    13081 acgggtcgcc agtcaggtca cggttctggt cgcggtcacg aatagaaacg tacatctgaa
    13141 taacgaggca gagaatacct agcgcaatca gtactgcacc gctggctgca atcatcagca
    13201 tggtgtggaa ctgcgggtca atctgctggc tcaaacgacg ggtcatgccc atgaagccca
    13261 gcgcgtacag tggcataaag gcaacgaaga agccgatgat ccagaaccaa aacgcgcgtt
    13321 taccccaggt ttcgttcagt ttgaaaccga acgctttagg ccaccagtag gtcatccctg
    13381 cgaagcagcc gaagaccacg ccgccgatga tcacgttatg gaagtgcgca atcaggaaca
    13441 ggctgttatg tagaacgaag tccgcgcccg gtactgccag cagcacgcca gtcataccgc
    13501 ccaccgagaa ggtgacgata aaaccgatag tccacagcat cgcagaatgg aacacgatgc
    13561 ggccctgata catggtgaac agccagttga agatcttcac cccggtcggg atggcgataa
    13621 tcattgtggt gataccaaag aaggcgttta cgttcgcgcc cgcacccatc gtaaagaagt
    13681 ggtgcagcca aacgatgaac gacagcacgg tgatacagac ggttgcccat accagcgagg
    13741 tataaccaaa cagacgttta cgcgagaagg ttgccgcaat ttcggagaac acaccgaaaa
    13801 caggcaggat caggatgtaa acttccgggt ggccccaggc ccaaatcagg ttgatgtaca
    13861 tcatcatgtt gccacccata tcgttggtaa agaaatgggt gcccagatag cgatccaggg
    13921 tcaacaacgc gacggtaacc gtcagaattg ggaaggaagc aataatcagt acgttcgcgc
    13981 acagtgatgc ccaggtaaat actggcatct tgaacatggt catgcccggt gcgcgcatct
    14041 tcagaatggt aacgaagaag ttgataccgg taagcgtcgt acctataccg gatagctgga
    14101 gactccatat ccagtaatcg acaccgactc ccggactgta ctctattccc gatagcggtg
    14161 gataggccag ccagccggtc tgcgcaaatt cgcccacgcc gagagaaacg ttaaccagaa
    14221 tcacaccaac aacggtaaac cagaagctta agttgttgag gaacgggaac gcaacgtcac
    14281 gcgcgccgat ctgcagcgga accaccaagt tcatcagacc gataacgaaa ggcatcgcca
    14341 cgaagaagat cataatcacg ccgtgcgcgg tgaagatctg atcgtagtgg tgaggtggca
    14401 ggaagcccgc ttcgcccgcc gaggcaagag cctgctggct acgcatcata atggcgtcag
    14461 caaaaccacg cagcaacatc acaatcgcca cgatgatata catgataccg aggcgtttat
    14521 ggtcgacgga ggtcagccac tctttccaca ggtaggtcca cttaccgaag taagtgatca
    14581 ggccaacgag cgccagacct cccaaaataa tgccagcgat cgtaaccatg acgataggtt
    14641 catggaacgg gactgcatca agtgataatt ttccgaacat ctttattctt cctcaacccc
    14701 tttaatgggc ggattccgcg tggctcatgt ccatgccttc cataccttcg tgtgcgctgt
    14761 gctcaccttc tggctgggtc atgtccatgc tcttaccgtg agccataaac ttgttaatta
    14821 catcggcaaa caagtttggt ttcacgttgg agaaatattc cacctggttg tattcgctag
    14881 gcgcggccag tttttcgaac gctgccatgt cagacatgga gttcggcgac tgtttcgctt
    14941 ttgcgaccca ctggtcgaat gcggcgcgat ccggtgttgc aatagctttg aacttcatgc
    15001 ctgagaagcc cgggccgctg tagctggcgg agataccgtc ataagtgccg ggttcgttgg
    15061 cgatcagatg caggcgagtc tgcataccgg ccatggcata aatctggcta cccagacgcg
    15121 gaatgaagaa ggagttcatc acggagttgg aggtcacttt gaagtacacc ggagtgttcg
    15181 ccgggaaagc gatttcattc acggtagcaa agccctgttc cgggtagatg aagaaccatt
    15241 tccagtccat ggaaaccact tcgatggtaa tgggcttctc gtcgtgtgcc agcggcttgc
    15301 taggctcaag agcgtgagtg gttttccagg tcagtaccgc aaggaagatg atgattaaga
    15361 taggtaccgt ccagaccaca gcttccactt tattggagtg tgaccagttc gggctgtact
    15421 tagcatcttt attgctcgca cggtacttcc aggcgaaacc aacagccatc aagattgcgg
    15481 gaataacgac aatcaacatc aggccaaatg ccgtcagtat cagtgaacgt tgctccagac
    15541 caatctgtcc tttgggatct aacagcgcag aattacagcc actgagcaat acagtgcctg
    15601 caaataatga caaccatccc aaacttttat tgtatttcct gagtctcatt taacgacctc
    15661 aattccacgg gatctggtgg cgtttaaaat gtgtgggcat tttacgggaa ggttacatta
    15721 ctgtaaacat gattaaattc ctgttacttg gatttggctg tcaggtcaca tatgttgcaa
    15781 aacatatcag gttgatttta agaagctgtt gcaaaagggc gttataacaa aaggggagtg
    15841 cagaagaagt ctggacgggc aattggtata atcaatgcga aataaaacaa ttatttaaca
    15901 aataattatc attggagtga caacttggtg gtgagaaaat aaagactcat tagctgaatc
    15961 gtgtaagaaa aatagaattt ataaatggcg ccaataattt ccaaacgcta agccgcacaa
    16021 aagaacaaat attaataagc gatgctgatg tttatttagc cgtaataatt acggcgagtg
    16081 attactacag ctaaataata tttacagatt acgtcagatg cgtttttcgt agcgccagat
    16141 aatccagcaa accaccaagc acgacgccag aaagggcgac taaaaccccg acttccagca
    16201 gagcaggcag gaaagagaag tgcgtcaaat ccagcgcgtc catcgtcaat agcagtaacc
    16261 acacggccaa caggctgacg cccgccgcca gtgtccacat ggcaaaggca taacctgccg
    16321 gatatgcggt acgggagata aagttgtcat ttactcgtgt atattcaagc gtctggcggc
    16381 aaaccagcag caaaataagc cctggtacgg cagcggcgac ggagaataga tagaatgtcg
    16441 accagccgtg tgcttcaaca aaccaacccg ccacggggcc gacataaact cgccctacag
    16501 cagaaagcgc tgagagcagg gcaaattgag tagcggaaaa tgacttatta catagcgtca
    16561 ttaacagcgc gacaaaggct gatgtgccca tcccgccaca gaggttttcg aaaaagacgg
    16621 ctgcgcccat gctgtagaga tgcttatcag taatcgacag cagccagtaa ccagcgttag
    16681 acgcaccttg taaaatgccg aaaatcagca gtgcccggaa cagtgacagg cgctgcatca
    16741 aaatcccacc gtacaatgcg ccaacaatgg tcgctagtaa gccaagcgtt ttgtttacca
    16801 cgcctacttc acccgcatca aacccgacgc cgcgaatcaa aaacgtggtt gtcaggctca
    16861 tggcgaatgc gtcgcccagc ttatacagca cgataagaag caaaataagc caggcattat
    16921 tgcgaccaaa gaaatctcgc agaggtgcaa caaccgcttg ttccagcgtt ttgggcacag
    16981 gaatggtgtc ggttggttct ggtgcaagca acgtcgcgat aatacagggg atcaacagcg
    17041 ccgccatcaa ccagtacatg ccctgccagc ccagccattt atctgccagc cacagggcta
    17101 ggccgccgga aaccagcatc cctaaacggt aacccagaac gctgattgcc gcgcccgcac
    17161 cacgttcttc tgccgggagc acatcggttt tccacgcatc gaagacaata tcctgggagg
    17221 cagagcaaaa agcgatcacc accgccagcg ccgccatcca gcggagttgg gtgcctggtt
    17281 cgagaaaacc catcgccgca atggcgacta ataacaggat ttgcgtggcg agcagccaac
    17341 cgcgccgccg cccaaaaaat gggggcgtgt agcggtccat cagcggtgac cagaggaatt
    17401 taaaaacgta agcctggcct accagagaga agaaaccaat ggttttgaga tcgatattct
    17461 cgaccgtcat ccaggcctgt aaggtgccgg atgtcagggc gagcggtagc ccggaagcaa
    17521 aacccaggat cagcaatatg gctgaacgcg gctgttgaaa aatacgtaaa tattgactgg
    17581 acatgggcgt ctacaggccc ggcttgcacc gggccagagg gcagaaatta acgcgcgttc
    17641 tgcttgatga attcgtgaat gctggtgtcc tgagacatat cagcgatggt atcggtcagc
    17701 acactgttaa ccgcatcggc gatatttttg ttggaggcct ggaacgcacc ttcaacgttg
    17761 tagctggcac gatagttttt ggtcattttg ttgccattct gcgcggtagc gatgatggcg
    17821 atatccgctt tggtagcgat gttgtagcgc acgttgccct gggacacgtc agcatacagc
    17881 tggctaacga tgatttgcag attaaccggg ccattcggac caaccatgta accacgcgcg
    17941 gtcatctgtt tttccagcac ttcttgcagc aggaaacgca gatcgcggga ggcggtcagg
    18001 gtaacgattt gattatcgcg ggtgactttt gccagcgcct gatcggtacg ctgatcggca
    18061 ccattaatgc ttacggtgac gcccatcagg cttggatcct gctgtggcag tgtaatcgtc
    18121 ggggaaactt caatagttgt tggcggtctt gcgcatcctg caagcataaa cagagcaact
    18181 aacgggaaga ggattttttt gaacatgttc gggctctcag agactcttaa gcgtgtttgg
    18241 taaaaattcc cgccatcata acattgccaa cggcgagggg aagtaggtaa ggcatgtaaa
    18301 ttcatcatgt tgacgaaata atcgcccctg gtaaaagaaa cgctgatgcg aggcctgtgt
    18361 ttcaatcttt aaatcagtaa acttcatacg cttgacggaa aaaccaggac gaaacctaaa
    18421 tatttgttgt taagctgcaa tggaaacggt aaaagcggct agtatttaaa gggatggatg
    18481 acatctcagc gttgtcggag gagatatttc atgatgatac gtgagcggat agaagaaaaa
    18541 ttaagggcgg cgttccaacc cgtattcctc gaagtagtgg atgaaagcta tcgtcacaat
    18601 gtcccagccg gctctgaaag ccattttaaa gttgtgctgg tcagcgatcg ttttacgggt
    18661 gaacgttttc tgaatcgtca tcgaatgatt tacagtactt tagcggagga actctctact
    18721 accgttcatg cgctggctct gcatacttac actattaagg agtgggaagg gttgcaggac
    18781 accgtctttg cctctcctcc ctgtcgtgga gcaggaagca tcgcgtaaaa acgcatttgc
    18841 aactgtcggc gcttttccag tatgttgcta aagattttat gaaaaacggc ctgcgggccg
    18901 ttttgttttg tctggatttt gcgctttttg cccagcattc agacgaaaat tgcccgggaa
    18961 ttgtgaaaaa atacgcgaca gcgcgcaata accgttctcg actcataaaa gtgatgccgc
    19021 tataatgccg cgtcctattt gaatgctttc gggatgattc tggtaacagg gaatgtgatt
    19081 gattataaga acatcccggt tccgcgaagc caacaacctg tgcttgcggg gtaagagttg
    19141 accgagcact gtgatttttt gaggtaacaa gatgcaagtt tcagttgaaa ccactcaagg
    19201 ccttggccgc cgtgtaacga ttactatcgc tgctgacagc atcgagaccg ctgttaaaag
    19261 cgagctggtc aacgttgcga aaaaagtacg tattgacggc ttccgcaaag gcaaagtgcc
    19321 aatgaatatc gttgctcagc gttatggcgc gtctgtacgc caggacgttc tgggtgacct
    19381 gatgagccgt aacttcattg acgccatcat taaagaaaaa atcaatccgg ctggcgcacc
    19441 gacttatgtt ccgggcgaat acaagctggg tgaagacttc acctactctg tagagtttga
    19501 agtttatccg gaagttgaac tgcagggtct ggaagcgatc gaagttgaaa aaccgatcgt
    19561 tgaagtgacc gacgctgacg ttgacggcat gctggatact ctgcgtaaac agcaggcgac
    19621 ctggaaagaa aaagacggcg ctgttgaagc agaagaccgc gtaaccatcg acttcaccgg
    19681 ttctgtagac ggcgaagagt tcgaaggcgg taaagcgtct gatttcgtac tggcgatggg
    19741 ccagggtcgt atgatcccgg gctttgaaga cggtatcaaa ggccacaaag ctggcgaaga
    19801 gttcaccatc gacgtgacct tcccggaaga ataccacgca gaaaacctga aaggtaaagc
    19861 agcgaaattc gctatcaacc tgaagaaagt tgaagagcgt gaactgccgg aactgactgc
    19921 agaattcatc aaacgtttcg gcgttgaaga tggttccgta gaaggtctgc gcgctgaagt
    19981 gcgtaaaaac atggagcgcg agctgaagag cgccatccgt aaccgcgtta agtctcaggc
    20041 gatcgaaggt ctggtaaaag ctaacgacat cgatgtaccg gctgcgctta ttgacagcga
    20101 aatcgacgtt ctgcgtcgcc aggctgcaca gcgtttcggt ggcaacgaaa aacaagctct
    20161 ggaactgccg cgcgaactgt tcgaagaaca ggctaaacgc cgcgtagttg ttggcctgct
    20221 gctgggcgaa gttatccgca ccaacgagct gaaagctgac gaagagcgcg tgaaaggcct
    20281 gatcgaagag atggcttcag cgtacgaaga tccgaaagaa gttatcgagt tctacagcaa
    20341 aaacaaagaa ctgatggaca acatgcgcaa tgttgctctg gaagaacagg ctgttgaagc
    20401 tgtactggcg aaagcgaaag tgactgaaaa agaaaccact ttcaacgagc tgatgaacca
    20461 gcaggcgtaa tttacgcagc ataacgcgct aaattcgcac aaaggcccgt caccgccagg
    20521 tggtgggctt tttttgtcat gaattttgca tggaaccgtg cgaaaagcct ctttcggtgt
    20581 tagcgtaaca acaaaagatt gttatgcttg aaatatggtg atgccgtacc cataacacag
    20641 ggactagctg ataatccgtc cataaggtta caatcggtac agcaggtttt ttcaatttta
    20701 tccaggagac ggaaatgtca tacagcggcg aacgagataa ctttgcaccc catatggcgc
    20761 tggtgccgat ggtcattgaa cagacctcac gtggtgagcg ctcttttgat atctattctc
    20821 gtctacttaa ggaacgcgtc atttttctga ctggccaggt tgaagaccat atggctaacc
    20881 tgattgtggc tcagatgctg ttcctggaag cagaaaaccc agaaaaagat atctatctgt
    20941 acattaactc cccaggcggg gtgatcactg ccgggatgtc tatctatgac accatgcagt
    21001 ttatcaagcc tgatgtcagc accatctgta tgggccaggc ggcctcgatg ggcgctttct
    21061 tgctgaccgc aggggcaaaa ggtaaacgtt tttgcctgcc gaattcgcgc gtgatgattc
    21121 accaaccgtt gggcggctac cagggccagg cgaccgatat cgaaattcat gcccgtgaaa
    21181 ttctgaaagt taaagggcgt atgaatgaac ttatggcgct tcatacgggt caatcattag
    21241 aacagattga acgtgatacc gagcgcgatc gcttcctttc cgcccctgaa gcggtggaat
    21301 acggtctggt cgattcgatt ctgacccatc gtaattgatg ccagaggcgc aactgtgccg
    21361 ctatacttat ccagggcggc acaacgctgt aagcggcttg cgcctgagaa tggcatttgc
    21421 gtcgtcgtgt gcggcacaaa gaacaaagaa gaggttttga cccatgacag ataaacgcaa
    21481 agatggctca ggcaaattgc tgtattgctc tttttgcggc aaaagccagc atgaagtgcg
    21541 caagctgatt gccggtccat ccgtgtatat ctgcgacgaa tgtgttgatt tatgtaacga
    21601 catcattcgc gaagagatta aagaagttgc accgcatcgt gaacgcagtg cgctaccgac
    21661 gccgcatgaa attcgtaacc acctggacga ttacgttatc ggccaggaac aggcgaaaaa
    21721 agtgctggcg gtcgcggtat acaaccacta caaacgtctg cgcaacggcg ataccagcaa
    21781 tggcgtcgag ttgggcaaaa gtaacattct gctgatcggt ccgaccggtt ccggtaaaac
    21841 gctgctggct gaaacgctgg cgcgcctgct ggacgtcccg ttcaccatgg ccgacgcaac
    21901 cacgctgacc gaagccggtt atgtgggcga agacgttgaa aacatcattc agaagctgtt
    21961 gcaaaagtgc gattacgacg tacagaaagc gcagcgcggg attgtctaca tcgatgaaat
    22021 cgacaagatt tctcgtaagt cagacaaccc gtctattacc cgtgacgttt ccggtgaagg
    22081 cgtacagcag gcactgttga aactgatcga aggtacggta gctgctgttc caccgcaagg
    22141 tggacgtaaa catccgcagc aggaattctt gcaggttgat acctctaaga tcctgtttat
    22201 ttgtggcggt gcgtttgccg gtctggataa agtgatttcc catcgtgtag aaaccggctc
    22261 cggcattggt tttggcgcga cggtaaaagc gaagtccgac aaagcaagcg aaggcgagct
    22321 gctggcgcag gttgaaccgg aagatctgat caagtttggt cttatccctg agtttattgg
    22381 tcgtctgccg gttgtcgcaa cgttgaatga actgagcgaa gaagctctga ttcagatcct
    22441 caaagagccg aaaaacgccc tgaccaagca gtatcaggcg ctgtttaatc tggaaggcgt
    22501 ggatctggaa ttccgtgacg aggcgctgga tgctatcgct aagaaagcga tggcgcgtaa
    22561 aaccggtgcc cgtggcctgc gttccatcgt agaagccgca ctgctcgata ccatgtacga
    22621 tctgccgtcc atggaagatg tcgaaaaagt ggttatcgac gagtcggtaa ttgatggtca
    22681 aagcaaaccg ttgctgattt atggcaagcc ggaagcgcaa caggcatctg gtgaataatt
    22741 aaccattccc atacaattag ttaaccaaaa aggggggatt ttatctcccc tttaattttt
    22801 cctctattct cggcgttgaa tgtgggggaa acatccccat atactgacgt acatgttaat
    22861 agatggcgtg aagcacagtc gtgtcatctg attacctggc ggaaattaaa ctaagagaga
    22921 gctctatgaa tcctgagcgt tctgaacgca ttgaaatccc cgtattgccg ctgcgcgatg
    22981 tggtggttta tccgcacatg gtcatcccct tatttgtcgg gcgggaaaaa tctatccgtt
    23041 gtctggaagc ggcgatggac catgataaaa aaattatgct ggtcgcgcag aaagaagctt
    23101 caacggatga gccgggtgta aacgatcttt tcaccgtcgg gaccgtggcc tctatattgc
    23161 agatgctgaa actgcctgac ggcaccgtca aagtgctggt cgaggggtta cagcgcgcgc
    23221 gtatttctgc gctctctgac aatggcgaac acttttctgc gaaggcggag tatctggagt
    23281 cgccgaccat tgatgagcga gaacaggaag tgctggtgcg tactgcaatc agccagttcg
    23341 aaggctacat caagctgaac aaaaaaatcc caccagaagt gctgacgtcg ttgaatagca
    23401 tcgacgatcc ggcgcgtctg gcggatacca ttgctgcaca tatgccgctg aaactggctg
    23461 acaaacagtc cgttctggag atgtccgacg ttaacgaacg tctggaatat ctgatggcaa
    23521 tgatggaatc ggaaatcgat ctgctgcagg ttgagaaacg cattcgcaac cgcgttaaaa
    23581 agcagatgga gaaatcccag cgtgagtact atctgaacga gcaaatgaaa gctattcaga
    23641 aagaactcgg tgagatggac gacgcgccgg acgaaaacga agccctgaag cgcaaaatcg
    23701 acgcggcgaa gatgccgaaa gaggcaaaag agaaagcgga agcagagttg cagaagctga
    23761 aaatgatgtc tccgatgtcg gcagaagcga ccgtagtgcg tggttatatc gactggatgg
    23821 tacaggtacc gtggaatgcg cgcagcaagg tcaaaaaaga cctgcgtcag gcgcaggaaa
    23881 tccttaatac cgaccattat ggtctggagc gcgtgaaaga tcgcatcctt gagtatcttg
    23941 cggttcaaag ccgtgtcaac aaaatcaagg gaccgatcct ttgcctggta gggccgccgg
    24001 gggtaggtaa aacctccctg ggtcagtcca ttgccaaagc caccgggcgt aaatatgtcc
    24061 gtatggcgct gggcggcgtg cgtgatgaag cggaaatccg tggtcaccgc cgtacttaca
    24121 tcggttctat gccgggtaaa ttgatccaga aaatggcgaa agtgggcgtt aaaaacccgc
    24181 tgttcctgct cgatgagatc gacaaaatgt cttctgacat gcgtggcgat ccggcttccg
    24241 cactgcttga agtgctggat ccagagcaga acgtggcctt cagcgatcac tacctggaag
    24301 tggattacga tctcagcgac gtgatgtttg tcgcgacgtc gaactccatg aacattccgg
    24361 caccactgct ggatcgtatg gaagtgattc gcctctccgg ttataccgaa gatgaaaaac
    24421 tgaacatcgc caaacgtcac ctgctgccga agcagattga acgtaatgca ctgaaaaaag
    24481 gtgagctgac cgtcgacgat agcgccatta tcggcattat tcgttactac acccgtgagg
    24541 cgggcgtgcg tggtctggag cgtgaaatct ccaagctgtg ccgtaaagcg gttaagcagt
    24601 tactgctcga taagtcatta aaacatatcg aaattaacgg cgacaacctg catgactacc
    24661 ttggtgttca gcgtttcgac tatggtcgcg ctgataacga aaaccgtgtc ggtcaggtaa
    24721 ccggtctggc gtggacggaa gtgggcggtg acttgctgac cattgaaacc gcatgtgttc
    24781 cgggtaaagg caaactgacc tataccggtt cgctcggcga agtgatgcag gagtctattc
    24841 aggctgcgtt aacggtggtt cgcgcgcgtg cggaaaaact ggggatcaac cctgattttt
    24901 acgaaaaacg tgacatccac gtccacgtac cggaaggtgc gacgccgaaa gatggtccga
    24961 gtgccggtat tgctatgtgc accgcgctgg tttcttgcct gaccggtaac ccggttcgtg
    25021 ccgatgtggc aatgaccggt gagatcactc tgcgtggtca ggtactgccg atcggtggtt
    25081 tgaaagaaaa actactggca gcgcatcgcg gcgggattaa aacagtgtta attccgttcg
    25141 aaaataaacg cgatctggaa gagattcctg acaacgtaat tgccgatctg gacattcatc
    25201 ctgtgaagcg cattgaggaa gttctgactc tggcgctgca aaatgaaccg tctggcatgc
    25261 aggttgtgac tgcaaaatag tgacctcgcg caaaatgcac taataaaaac agggctggca
    25321 ggctaattcg ggcttgccag cctttttttg tctcgctaag ttagatggcg gatcgggctt
    25381 gcccttatta aggggtgttg taaggggatg gctggcctga tataactgct gcgcgttcgt
    25441 accttgaagg attcaagtgc gatataaatt ataaagagga agagaagagt gaataaatct
    25501 caattgatcg acaagattgc tgcaggggct gatatctcta aagctgcggc tggccgtgcg
    25561 ttagatgcta ttattgcttc cgtaactgaa tctctgaaag aaggggatga tgtagcactg
    25621 gtaggttttg gtacttttgc cgttaaagag cgtgctgccc gtactggccg caacccgcag
    25681 accggtaaag agatcaccat cgctgccgct aaagtaccga gcttccgtgc aggtaaagca
    25741 ctgaaagacg cggtaaacta agcgttgtcc ccagtgggga tgtgacgaag ttcaagggcg
    25801 catctactga tgtgcctttt ttatttgtat tcggtgactt tctgcgtctt gtgggctgac
    25861 aattgccccc gtttcttgtc acaataggcc tttgcgcgca tcgatacgtt gcgtgaggta
    25921 cacagtcatc tacagcggag tgttgttaca ccatgatgga cagcttacgc acggctgcaa
    25981 acagtctcgt gctcaagatt attttcggta tcattatcgt gtcgttcata ttgaccggcg
    26041 tgagtggtta cctgattggc ggaggcaata actacgccgc aaaagtgaat gaccaggaaa
    26101 tcagccgtgg gcaattcgag aatgccttca acagcgagcg taatcgcatg cagcaacagc
    26161 tgggcgatca atactccgag ctggcagcga acgaaggcta tatgaaaacc ctgcgtcaac
    26221 aggtgctgaa tcgtctgatc gacgaggcgc tgctggatca gtacgcacgt gagctgaaac
    26281 tgggtatcag cgatgagcag gttaaacagg cgattttcgc gaccccagcc ttccaggttg
    26341 atggcaaatt tgataacagc cgctataacg gtatcctcaa ccagatgggg atgaccgccg
    26401 atcagtacgc ccaggcgctg cgtaaccagc tcactaccca acagctgatt aacggcgttg
    26461 ctggtaccga ttttatgctg aaaggtgaaa ccgacgagct ggcggcactg gtcgctcaac
    26521 aacgcgtggt gcgtgaagcg actatcgatg ttaacgcgct ggcggcgaag cagcctgtga
    26581 ccgaacagga aattgccagc tactacgaac aaaacaaaaa caatttcatg acgccggaac
    26641 aattccgcgt gagttacatc aagctggatg ccgcaacgat gcagcaaccg gttagcgatg
    26701 cggatctcca gagctactac gatcagcatc aggatcaatt cacccagccg cagcgtaccc
    26761 gttacagcat catccagacc aaaactgaag atgaagcgaa agcggtactt gatgagctga
    26821 ataaaggcgg tgattttgct gcattagcca aagaaaaatc tgccgatatt atctctgctc
    26881 gtaacggcgg cgatatgggt tggttagaag atgccactat cccggatgaa ctgaaaaatg
    26941 ctggtctgaa agaaaaaggc caactgtctg gtgtcatcaa atcttcggtc ggtttcctga
    27001 ttgtacgtct ggacgacatt cagccagcga aagtgaaatc gttagacgaa gtacgtgacg
    27061 atgtcgcggc gaaagtgaaa cacgaaaaag ccctcgatgc gtactacgcg ctgcagcaga
    27121 aagtgagcga tgcggcaagc aacgacaccg agtctctggc cggtgcagag caagctgccg
    27181 gcgttaaagc cactcagacg ggttggttca gcaaagagaa cctgccggaa gagttgaact
    27241 tcaagccggt tgctgacgct atctttaacg gcggtctggt aggtgaaaac ggcgcgccgg
    27301 gcatcaactc tgacatcatc accgtagacg gcgaccgcgc attcgtgctg cgcatcagcg
    27361 agcacaaacc ggaagcggtg aaaccgttgg cagatgttca ggaacaagtt aaggcactgg
    27421 ttcagcacaa caaagctgaa caacaggcga aagtggatgc tgagaaactg ctggttgatt
    27481 tgaaagccgg caaaggtgcg gaagctatgc aggctgccgg tctgaaattt ggcgagccga
    27541 aaaccttaag ccgttccggt cgtgacccga ttagccaggc ggcgtttgca ctgccactgc
    27601 cagcgaaaga caaaccgagc tacggtatgg cgaccgatat gcaaggtaat gtggttctgc
    27661 tggcgctgga tgaagtgaaa caaggttcaa tgccggaaga tcagaaaaaa gcgatggtgc
    27721 agggtatcac ccagaacaac gcacaaatcg tctttgaagc tctgatgagt aacctgcgta
    27781 aagaggcgaa aatcaaaatt ggcgatgcgt tggaacagca ataatcctga agccgcctcg
    27841 caaaaaaatg ctttgcagct gtaacaagaa aaggtcgctt tcgcggcctt ttccatttct
    27901 gaacattgcc atttgtttac tgttttcact gccgttaagg tgattccact gttaacaaac
    27961 aaggagaaaa cagtatgaaa cacggaatta aagcactgct cattaccctg tccctggcct
    28021 gtgccggaat gtctcatagc gcgctggcgg cagcttctgt ggcgaaaccg acggcggtag
    28081 aaaccaaagc ggaagctcct gcagcacaaa ataaagcagc agtaccggcg aaagccagtg
    28141 acgaagaagg ctcccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
    28201 tgaatggcgt tggcctgaag aaagcgcaag cgattgtcag ttatcgcgaa gagtacggtc
    28261 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
    28321 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
    28381 aagaggtcat accagttatg acctctgtac ttataacagt ttttataaaa acaacgtaag
    28441 gttattgcgc tatgcaaaca caaatcaaag ttcgtggata tcatctcgac gtttaccagc
    28501 acgtaaacaa cgcccgctac cttgaatttc tcgaagaagc ccgctgggat gggttggaaa
    28561 atagcgacag ttttcactgg atgacggccc ataacatcgc tttcgtggtg gtgaatatca
    28621 atattaacta tcgtcgtccg gcggtattaa gcgacttgtt aaccattacg agccagttac
    28681 agcaattaaa cggtaaaagc ggcatcttaa gccaggtcat tacactggag ccggaagggc
    28741 aggtggtagc ggatgcgctt attacgtttg tttgtattga tcttaaaacg cagaaagcat
    28801 tagctctgga aggggaattg cgcgaaaagc tggagcagat ggttaagtaa acgttttgtg
    28861 gtgccggatg ctcaagccgc atccggcgac acccggaata attacttcaa cccggttttc
    28921 tgcttcatcg ctgccatcac cgtcggttta tcggccagat aatgattcaa accgttggcg
    28981 cgtaaattac atgccgcaca atgaccgcaa ccgtcgcctt taatgccgtt atagcaggtc
    29041 aacgtttcgt tacggactaa atccagtttg ccgtaataat ctgccagcgc ccaggtttcc
    29101 gctttatcaa tccacatcag cggcgtttca aaacgaatat ctttcgccat gcccaaactg
    29161 acggcatggt ttagtacttt cacaaactca tcgcggcaat ccgggtagcc ggagaaatcc
    29221 gtttcgcaga cgccggtaat tacggcttct gcttttacct gatacgcata tattgccgcc
    29281 agcgtcagga acaaaatatt acgccctggg acaaacgtat tcgggatgcc atcggcttca
    29341 ggttcataat caggcaccgg aatgctgtca cgcgtcaggc tgctgaccgc cagctcgttg
    29401 agcagcgtta catcaagaac cttatgcgcg cgtgccccca gtttcagcgc cagttcgcgt
    29461 gccacgtcga tttctgcgcg atggcgctga ccgtaatcga acgtcacgca atggacttca
    29521 tcatattgtt gtaatgcctg caccagacag gtggtggaat cctggcctcc actgaacaca
    29581 acgacagcac gtttcataga taatcctgct tgaacaataa aagcgttatg gtaacgcctg
    29641 cgattaaccc ggaccagcta ttcattgcga cggcgcggga agccaggctt cggtaaattc
    29701 aaaccagccg cgcggtgtca gtcgcacacc gttcacgccg ggaggggcac taatgcgata
    29761 gtgatagttg aacagcggcg tcagcgtcgc atctgtcatt aactggctaa aaaccgcttt
    29821 cagggcatta aatcggtttt cctcatcagg cattacttgc accgcgtcca gtgtcgattg
    29881 tagatgtgcg tatgctggag cgtcgaaaac atgtggccac agcggatcgc agcgcagcca
    29941 ttgctccaga gtatattccg gtgcttcgcc aattaatctg tcgcccatca tgaggtctgc
    30001 gtgtgccagt agggtcgtgt cgtcccagtt ttttgcgtta tgaaaaataa ttgtgagttc
    30061 acagccttcc gctgccagtg tcgcctgtag gcgttctgcc atggtatgaa gttctatcgg
    30121 gaggtgataa accagcgtca aggttttcgg tagtttgact tcatccggta cttgccaatg
    30181 cggaatagtc cagcctggca gtaatgcatg actggcggtg atcaggtttt ctccgacttc
    30241 taacgtttgt aataaaccgg attgatgaat aatggagatc acttttcgcg cctgccagag
    30301 ggagagtcgg ggacttttgc gcaacgttaa atagcaaaaa cctaaactga tgccactact
    30361 gacctggctg acccgttgca gctcctccgg tttgccgatg gtgatttgca cgggatgccg
    30421 acaactggtt cccaaatctt tttcgaaaag cggcggagtt atccagtact caaccgcttt
    30481 aagcagcgga tgacgtaaat ggtaataatc atggctttcc aggcgcacca gttcagctgt
    30541 gaattgtgtt aagcgaaaag gacccgtgcc aatcagtggg aattgcggat gcgccagatg
    30601 gctgcaatag ctcgccagcc ggtgcgcaag ccagtaatcg gggcgatgta aaaagaaggt
    30661 cagacactgc ggatgggtga cctcaatacg cttcacgcta ataaataatt gatccagtgc
    30721 tggcagttgt aacagcatca ataatcgctg gtgtaagtgt gaggctttta ctgcatcgcc
    30781 gttatgccag tgtagggttg aacgaagata aaagtcccag cgtaacccgt cagcagaggt
    30841 ttcccagtga tgcgctaaat cgccaatcgg gcgctgggta ttattatcga agcgggtcag
    30901 gccggaaaat atctgtccgg cgagatgctg ctcggcacgg ccgggcaaaa agcccggttg
    30961 tagcggttcg agcgggcgat agtagggaat acgcaacgtg ggtgtatcgt tttgccattg
    31021 tccgcccata aacggctgta acagagtgcg cagctcacct ggggccagtt gcgccagctc
    31081 cagcacatct tgctgctttc cggtttccag tgccttttcc atcatcgcat tgcgtagcga
    31141 ttctggcgtg accagaaagc gtaattgtcc gcgctttccg cgtcctgact gcgcctgcca
    31201 ctccagccat cccgcttcct gtgcctgacg caacagcgta cgaacatggc gttcgctgca
    31261 aaaacagcgt tcggccagtt cgctgacggt gacggtttgc ggctttccgg cggaaggttg
    31321 ccacagacgt tgatactggt taaggcggtt gagcagtcgc ataaaaaccc ggaacaatat
    31381 tatttaacta ttcactatta cttccgtata tatcaggtga tactcaatca ccattaaccg
    31441 tgtcacagag tggagaaaga aatggctcgt ctggcagcat ttgatatgga tggcacttta
    31501 ttgatgcctg accatcattt aggtgagaaa accctctcta ctttggcgcg actgcgtgaa
    31561 cgcgacatta ccctcacttt tgccacgggg cgtcatgcgc tggagatgca gcatattctc
    31621 ggggcgatat cgctggatgc gtatttgatt accggcaacg gaacgcgcgt gcattctctg
    31681 gaaggtgaac ttttacatcg tgatgattta cctgcggatg tcgcggagct ggtgctgtat
    31741 cagcaatggg atacccgagc cagcatgcat attttcaatg acgacggttg gtttaccggg
    31801 aaagagatcc ctgcgttgtt gcaggcattt gtctatagcg gttttcgtta tcaaataatc
    31861 gatgtcaaaa aaatgccact cggcagcgtc accaagatct gcttctgtgg cgatcacgac
    31921 gatcttacac gtttgcagat ccagctacac gaagcattag gcgagcgtgc acatttgtgt
    31981 ttttccgcca cggattgcct tgaagtgctg ccggtgggct gcaataaagg cgctgcactg
    32041 acggtgctga cccaacattt aggtttatcg ttgcgcgatt gcatggcctt tggtgatgcg
    32101 atgaacgatc gcgaaatgtt aggcagcgtc ggtagcggat ttattatggg caatgcgatg
    32161 ccgcaactgc gcgcggagct cccgcattta ccggtgattg gacattgccg aaatcaggct
    32221 gtctctcact atttgacgca ctggctggac tatccacatc taccttattc ccccgaataa
    32281 cgagatccct tccagcaccg ggcaattgcc cggttttttt gcgttgaatt tgtcattttg
    32341 tgccgtggtg tttaaaccgc acagaataaa ttgtcgcgat ttcaccttta aaatagaatt
    32401 aaaagagaaa aaaattctcc gtggaagggc tatgttagat aaaattgacc gtaagctgct
    32461 ggccttacta cagcaggatt gcaccctctc tttgcaggca ctggctgaag ccgttaatct
    32521 gacaaccacc ccctgctgga agcgcctgaa acggctggag gacgacggta tccttatcgg
    32581 caaagtcgcc ctgctggatc cggaaaaaat aggcctcggc ctgaccgcct ttgtgctgat
    32641 aaaaacgcaa catcacagca gcgaatggta ttgccgcttt gtcacggtgg ttaccgaaat
    32701 gccagaagtg ctggggttct ggcgcatggc tggtgaatac gattatctga tgcgcgtcca
    32761 ggttgccgac atgaaacgct acgacgagtt ttataagcgt ctggtaaaca gcgtgccggg
    32821 gctgtcggac gtcacttcca gcttcgcgat ggaacagatt aaatacacca cttctttacc
    32881 catcgaataa atatccagaa tcaggtcagg acacaacgcg tgcgattatt tgctcaatta
    32941 agctggtatt tccgtcggga atggcgtcgc tatctcgggg ctgtcgcctt gcttgtcatt
    33001 atcgcgatgc tgcaactggt tccaccaaaa gtggttggta ttgttgtcga tggcgtgaca
    33061 gaacaacact ttactaccgg gcagatcctg atgtggatcg ccaccatggt gctgattgcc
    33121 gttgtggttt atctcctgcg ttacgtctgg cgcgtattgc tgtttggtgc gtcttatcaa
    33181 ctggctgttg aactgcgtga agattattac cgtcagctaa gccggcagca tcctgagttt
    33241 tacctgcgtc atcgcacggg tgacctcatg gctcgtgcga ctaatgacgt cgatcgcgtc
    33301 gtgtttgccg ccggagaagg ggtgctgacg ctggtggatt cactggtaat gggctgcgct
    33361 gtgttgatta tgatgtctac gcaaattagc tggcagttga ccttatttgc cctgttaccg
    33421 atgccagtga tggcgatcat gattaagcgc aacggcgatg ctttgcatga acgctttaag
    33481 ctggcacagg cggcgttttc cagtcttaat gaccgcaccc aggaaagcct caccagtatc
    33541 cgcatgatca aagcctttgg tctggaagat cgccagtcgg cgttatttgc cgcggatgcc
    33601 gaagataccg gcaaaaaaaa catgcgggtg gcgcgtattg atgctcgttt cgacccgacc
    33661 atctatatcg cgattggtat ggcgaacttg ctggcgattg gcggcggtag ttggatggtg
    33721 gtgcagggca gtttaacgct gggccagctc accagtttta tgatgtattt aggtctgatg
    33781 atttggccaa tgctggcgct ggcatggatg tttaacattg tggaacgtgg tagtgctgcg
    33841 tacagccgta ttcgcgcgat gctggcggaa gcgccggtgg tgaacgatgg tagcgaaccc
    33901 gtgccggaag ggcgtggcga actggatgta aatattcacc agttcacgta tccgcagact
    33961 gaccatcctg cgctggaaaa cgtcaatttc gccctgaaac ccggtcagat gctgggtatc
    34021 tgcgggccga ctggttccgg caaaagtacc ctgttgtcgc tcattcagcg tcatttcgac
    34081 gtcagcgagg gggatattcg ctttcatgat attcctctga cgaagttaca actcgatagc
    34141 tggcgtagcc gcctggcggt agttagccag acgccattcc ttttttctga caccgtggcg
    34201 aataacatcg cgctgggttg cccgaatgcc acccagcaag agattgagca tgtcgcgcgg
    34261 ttagccagcg tacatgacga tattttgcgt ctaccgcaag gttacgatac agaggtgggc
    34321 gagcgcggtg tgatgctttc cggcgggcaa aaacagcgta tctccattgc tcgtgcgtta
    34381 ttagtcaacg cggaaatcct catccttgat gatgcgcttt cggcggtgga cggacgcact
    34441 gagcaccaga tcctgcataa cctgcgtcag tgggggcagg gaagaacggt aatcatcagt
    34501 gcccatcgcc tttctgcact gacggaagcc agtgaaatta ttgtgatgca gcacggacat
    34561 atcgcccagc gtggcaatca tgatgtgctg gcacaacaaa gcggctggta tcgcgatatg
    34621 tatcgctatc aacaactgga ggcggcgctc gacgacgctc cggaaaatcg cgaggaggcc
    34681 gtcgatgcgt agttttagcc aactgtggcc gactctcaag cgcctgttag cgtacggttc
    34741 accgtggcgt aaaccgctgg ggattgcggt cctgatgatg tgggttgcgg cggcggcaga
    34801 agtcagtggg ccgctgctta tcagctattt tatcgacaat atggtagcga aaaataacct
    34861 gccgttgaaa gtggttgcag ggctggctgc ggcgtatgtt gggctgcaac tgtttgccgc
    34921 cgggctacat tacgcgcagt cgctgctgtt taatcgggcg gcagtaggcg tagtgcaaca
    34981 gttgcgtacc gacgtgatgg atgctgcgtt acgccagcca ttaagcgagt ttgataccca
    35041 acccgtcggg caggtgattt cccgcgtcac taatgacact gaagtgatcc gcgatctcta
    35101 cgttaccgta gtggcaactg tcctgcgcag tgccgcactg gtgggcgcga tgctggtggc
    35161 gatgttcagc ctcgactggc gaatggcgct ggtggcgata atgattttcc cggtggtgct
    35221 ggtggtaatg gtgatatacc agcgttacag cacgccaatt gtccgtcgtg tgcgcgccta
    35281 tctggcggat atcaacgacg gctttaacga aatcatcaat ggcatgagcg ttatccagca
    35341 gtttcgtcag caggcgcgat ttggcgaacg tatgggtgag gccagtcgtt cacactatat
    35401 ggcgaggatg caaaccctgc gcctcgacgg ttttctgctg cgtccgctgc tgagtctgtt
    35461 ttcatcgctc attctctgtg gcttgttgat gctgtttggc ttctccgcca gcggcaccat
    35521 tgaagtgggc gtgttgtatg cgtttatcag ctatcttggg cgacttaacg aacccttaat
    35581 cgaactgacc acgcagcagg cgatgctgca acaggctgtt gttgctggtg agcgcgtgtt
    35641 tgagctgatg gacggaccgc gccagcaata tggcaatgat gatcgcccgt tacagagtgg
    35701 caccatcgaa gtcgataacg tgtcatttgc ttatcgcgat gacaatctgg tgctaaagaa
    35761 cattaatctc tctgtgcctt cgcgtaactt tgtggcgctg gtcgggcata ccggcagtgg
    35821 taaaagtacc ctcgccagtt tattgatggg ctattacccg ctaacggaag gtgagattcg
    35881 ccttgatggt cgtccattaa gttcgctaag tcacagcgcg ctgcgccagg gcgtggcaat
    35941 ggtgcagcaa gatccggtgg tgctggcgga taccttcctc gccaacgtga cgctggggcg
    36001 ggatatctcc gaagaacgcg tctggcaggc gctggaaacc gtgcaactgg cggagctggc
    36061 acgcagtatg agcgacggga tttatacgcc gctgggcgag caggggaata atctctctgt
    36121 cgggcaaaag caactgctgg cactggcgcg cgtgctggtc gagacgccgc aaatcctgat
    36181 ccttgatgag gcaaccgcca gcattgactc cggtactgaa caggcgattc aacatgctct
    36241 ggcggcggtg cgtgaacata ccacgctggt agtgattgct caccgcttat cgactattgt
    36301 tgatgccgac actattctgg tgcttcatcg tgggcaagcc gtggagcagg gcactcacca
    36361 gcaactgctg gcggcccagg gacgctactg gcagatgtat caactgcaac ttgcgggcga
    36421 agagcttgca gccagcgtgc gtgaagaaga atcattgagc gcctgaatag cgcaatatct
    36481 catcgttggt gcaaaaatgt aacgcactgt gcactgtcat agtgcgtttt cattttcaat
    36541 cttcttaact tcctgctctc tttctcgttt ttcatttctg gcacaccgct tgcaatacct
    36601 tcttcgtgta gcagaaccat taccgaattc tgaccggagg ggatctatga agctggtgac
    36661 cgtgataatc aaaccattca agctggaaga cgttcgtgaa gcgttatctt ccattggtat
    36721 tcagggcctg accgtcaccg aagtgaaagg tttcgggcgt cagaaagggc atgccgagct
    36781 gtaccggggg gcggaataca gcgtcaactt cctgccaaaa gtaaaaattg atgtggcgat
    36841 tgctgatgac caactcgatg aagtgatcga tatcgtcagt aaggcggctt acaccggaaa
    36901 aattggcgac ggcaaaatct tcgtcgctga attgcaacgc gtcattcgta ttcgtaccgg
    36961 cgaagccgac gaagcggcgc tgtaatctct ggcacacagc aacaggaacg aaaaatgaag
    37021 atagcgacga taaaaactgg gcttgcttca ctggcgatgc tgccgggact ggtaatggct
    37081 gcacctgcgg tggccgataa agccgacaat gcgtttatga tgatttgtac tgcactggtg
    37141 ctgtttatga ctattccggg gattgccctg ttttacggtg ggttgattcg cggcaaaaac
    37201 gtgctgtcga tgctgacgca ggtgacggtg acgtttgcac tggtctgtat tctctgggtg
    37261 gtttacggtt actcgctggc gtttggtgag ggcaacaact tcttcggcaa cattaactgg
    37321 ttgatgctga aaaacatcga actgacggcg gtgatgggca gcatttatca gtatatccac
    37381 gtagcgtttc agggatcgtt tgcctgcatt accgtaggct tgatagttgg ggcgctggcg
    37441 gaacgaatcc gcttctcagc tgtgttgatt ttcgtggtgg tatggctgac gctctcttac
    37501 attcctattg cgcatatggt gtggggcggt ggtttgctgg cttctcacgg tgcgctggat
    37561 ttcgcgggtg gcacggtggt gcatattaac gccgcaatcg ccggtctggt gggcgcgtat
    37621 ctgataggca aacgcgtggg cttcggtaaa gaggcgttta aaccgcacaa cctgccgatg
    37681 gtcttcaccg ggactgccat tctctatatc ggttggtttg gctttaacgc cgggtcagcg
    37741 ggcacggcga atgaaatcgc ggcactggca tttgtgaata ctgtggtcgc aacggcggcg
    37801 gcaattcttg gctggatctt cggtgaatgg gcgctgcgtg gtaagccttc actgctggga
    37861 gcgtgttctg gcgcgattgc cggtctggtc ggcgtgacgc cagcgtgtgg ctacattggt
    37921 gttggcggcg cgttgattat cggcgtggta gctggtctgg cgggcttgtg gggcgttacc
    37981 atgctcaaac gcttgctgcg ggtggatgat ccctgcgatg tcttcggtgt gcacggcgtt
    38041 tgtggcattg tcggctgtat catgactggg atttttgccg ccagctcgct gggcggcgtg
    38101 ggcttcgctg aaggtgtgac gatgggccat cagttgctgg tacagttgga aagcattgcc
    38161 attacgattg tgtggtctgg tgtggtggca tttatcggct acaaactggc ggatctgacg
    38221 gttggtctgc gtgtgccgga agaacaagag cgagaagggc tggacgtcaa cagtcacggc
    38281 gagaatgcct ataacgcata acaagcactg caaaaaacag ccggacggtt ttcacctccg
    38341 gctatttttt taattgtgat tacgcatcac cccttcctga acggtcgagg caaccagtac
    38401 gccgtcctgg gtataaaact caccgcgcac aaagccacgt gcgctggacg ccgaggtgct
    38461 ctccacgcta tacagcagcc attcattcaa attaaacggg cgatggaacc acatggaatg
    38521 gtcaatggtg gcaatctgaa tccccggttc gagaaaaccg ataccgtgcg gctgtagagc
    38581 taccggcagg aagttaagat cagaagcgta accgagcaga tactgatgaa cgcgcaggtc
    38641 atccggcacg ctaccatttg cgcggatcca cacctgacga tgtggttctg cgacgtgacc
    38701 tttcagtggg ttatgaaact ccaccggacg gacttccagc ggacgatcgc agatgaattt
    38761 atctttaagc actggcggca gcaggtgcgc cagcgattgg gcgatttgcg tttccgaagg
    38821 caggccatca ggcgctggcg cggacggcat tgttttttga tgttcgaaac ccgcttctgg
    38881 tgcctggaaa gaggccgtca tataaaaaat cggtttgccg ttttgaatag cagcaacccg
    38941 gcgggcgctg aagctgttac cgtcacgcag cgtttcgaca tcataaataa tcggcttctt
    39001 actatcgcca gggcgaagaa agtagctgtg aaacgaatgt accagccgct cttcagggac
    39061 ggtctctttt gcagcataca aggcctgacc cacgacctgg ccgccaaata cctggcgtaa
    39121 acctaaatct tcactctggc cgcgaaagag tccttcctca attttttcca gatttaacaa
    39181 tgtcagtaaa ttttttagcg cctgactcat ataactctcc agtaacaaag ctgccgcagc
    39241 aagccaaagt gagttgagta taacgcaaat ttgctactgg tccgatgggt gcaatggtct
    39301 gaattacggg ctaattacag gcagaaatgc gtgatgtgtg ccacaattgt tgacgttact
    39361 attttgttaa ccactcttcc ggcgaggaaa gttagcccgc tggtgcattg ataataagga
    39421 gaaatgaatg aaactcgtgc acatggccag tggtttagcg gttgcgattg cgttggcggc
    39481 ttgcgcagat aaaagcgcgg atattcagac gccagccccg gctgcaaata cgtctatttc
    39541 agcaacacaa caacctgcta tccagcaacc gaatgtctcc ggtaccgtct ggatccgtca
    39601 gaaagtcgca ctgccgcctg atgctgtgct gaccgtgaca ctttctgacg cgtcgttagc
    39661 cgatgcaccg tcaaaagtgc tggcgcagaa agcggtgcgt actgaaggta aacagtcacc
    39721 attcagcttt gttctgccat ttaacccggc agatgttcag ccgaacgcgc gtattctgtt
    39781 gagtgcggcg attaccgtga atgacaaact ggtatttatt accgataccg ttcagccggt
    39841 gatcaaccag gggggaacca aagccgacct gacattggtt ccggttcagc aaaccgccgt
    39901 gcctgtccag gccagtggtg gcgcaacaac taccgtacca tcgacttcac cgacccaggt
    39961 gaatccatct tcggcagttc ctgcccctac gcaatactaa gcgttactaa cccctttcca
    40021 tctggagagg ggttagtagt tccagcgata gcgctgtaag tcgatttgcc cgcttcccga
    40081 taccatcaca ccttctgcca gtaatgcctg tcgttgacgc tgtaaatccg gtccggttag
    40141 cgaaatggtg ccgtggcgat taaccacccg gtgccagggc aaggtgctgc cttcggggag
    40201 acgctttaac acaccgccaa cctggcgcgc ggcgcggggc gatcccgcca gtttcgccac
    40261 atcaccgtaa gtggtgacat acccttcggg aatagcggct acgatttgcc agacgcgttg
    40321 gggaaatgaa tcttcttttt ccatcttttc ttcctgagat aatttttcag cataatctgg
    40381 aaaaacgccc gagtgaagcc gcattgcgca agaaaccagc atctggcacg caatgggttg
    40441 caattagccg gggcagcagt gataatgcgc ctgcgcgttg gttctcaacg ctctcaatgg
    40501 gggctctgtt ggttctcccg caacgctact ctgtttacca ggtcaggtcc ggaaggaagc
    40561 agccaaggca gatgacgcgt gtgccgggat gtagctggca gggcccccac ccatttctgc
    40621 ctcccaccgt ttcgtcaaaa aatcccaaca tgactaaact ttaaccacaa ctgacgtcgc
    40681 aagaattgtc tggctgcgca gtacgcttcg gaggtatgtc tgatgaagta tgttgatggt
    40741 tttgtggttg ccgttcctgc cgataaaaag gatgcctatc gggaaatggc cgctaaggcc
    40801 gcgccattgt ttaaagagtt tggcgcgctt cgtattgtcg aatgctgggc cagcgatgta
    40861 ccggatggca aagtgaccga ttttcgtatg gcggtgaaag cggaagagaa tgaagaggtt
    40921 gtctttagct ggattgaata cccttcaaaa gaggtccgcg acgcagctaa tcaaaagatg
    40981 atgtcggacc cacggatgaa agagttcggc gagtccatgc cgtttgacgg caagcgaatg
    41041 atctatggcg gattcgagtc aatcatcgac gaataggtag cgtgacgggc tgtgcggcgt
    41101 gccgtcagcc caaaaattca caaatgttgc tcggcccagc gtaagaaatc ttctttcggt
    41161 aatgccttgc tgtagagcca gccctgaccg tagtgcacgc catgctggcg tagccactct
    41221 tcttgtttac tggtttcgat tccctccgct accattttca gtttcagtgt ttttgccatt
    41281 tcgatgatat gcggcgtgac atttttatat tccagcgcat caacgaaaga tttatcgatc
    41341 ttcagaatgt cgacatccag atcctgtaaa taacttaaac ttgaataccc cgtaccaaaa
    41401 tcatcaagat aaatttcatg gcccgcctcc cggtagcgag aaattatcgg ggcgctggtt
    41461 ttcggatcgg caaactcgcg ttcagtgagt tcaagcgcga tctgtctggg attaacctga
    41521 tagtgattga tcatttcacg cagcaattgc gggatttttt ccgaggtgag cacggtggat
    41581 tcaagattga tcgaaatatg ctgctgtgga tgctgacgca gccagtcgcc catatcttca
    41641 aagacgcttc ttataatcag tagcgtcaat ggctcagaaa ggcccgtttg ctgtgccagc
    41701 ggaataaaac tatctggtga caaccaacta ccgtctgtct gcggccagcg cgccagtgcc
    41761 tcagcaccga caattttgcc attggctaag gagacaatcg gctgatagtg cacgcaaata
    41821 tcacgatttt cgatagcatc ctgcagccga tgatgcggtg actggatgcg gcgcaaaatg
    41881 cgcagcacaa acatcgctgc cagcaggcca atcaccaacc cgagcggtaa ccaaataaag
    41941 acttgccgat gccagccttt ctgcaacatt ttcgatgaag cccaggtgat aattgaaata
    42001 ttcatctccg ggaagggcag gatatcgtaa atgattccat tattttcgat atgctcaccg
    42061 ggtgtttttt gtagcctggt aataattccc tgagcaattt catcgctgct ggttatgaca
    42121 acgttatggg cattgccaat aatggcggca tcaatttgcc atgagctata gggaatgaca
    42181 tcaataaagg aagcggggtc gatcatgacg acataatgtg ccgttcccat ggcgaccatg
    42241 taacggataa tgcctaaatc gttatgtgat gttaaccaga cacgataacc atctttcgaa
    42301 attttacctg gctcggggaa ggtatcgggc ggactttcat gctccagaga cgaacactgg
    42361 ggaacgttgt tatcgatata aaccacttcc tgaatatagc gataactgta agatacccga
    42421 cgcatttcca tgagatgggc ttcactacag tcagcgcctt gccatctttc cagctcttgc
    42481 agcgcatctt tcccttgtgt cgccacctta ttggcccgaa tagcgacgcg ggaggaatag
    42541 gtatccagct cttcaataaa cgatgtttct acctgctgat gggccagcca gatgcttaag
    42601 ccgacaggca gcaatactga aagaatcagt actcccgaaa taaggccgac cagatgtcgt
    42661 gttctcacca gcatatcctt attaacacag tgtagggaaa gtatatctga ttgtggaaaa
    42721 aggctggcag gagataagga ggtagagctg gtatttcatg aagtgatggg tggaaacgat
    42781 cgttgtcatt tgaccaggcg cagagcttta cgcctggtat gtaatcctaa cttaagatgt
    42841 tgattcggcg cgagcgaggg taaaaatatc gtaaaaagac aattcacctt tacggacgat
    42901 cattttttgc agttgttcct tatgaacatc cgcgacacgc ggagagttca agatggcttc
    42961 aaccagcgcg gcaggtacaa agcgcgtgtt gtaccattcg ccgttatagc aaacgcggaa
    43021 atccaacacg tcgatatctt catagcggta gcgtgggtag acatcgaaaa agaagaaacc
    43081 attaagcagg ataaataata caaccagtag ccagacagag cctgaaagcg tttcggactg
    43141 caacatcacc gccagggtgg caaaaaaagc aacgtacata ccgataaaca gccccggatg
    43201 tttacggata aaactgatac taaagcgggg tttgttgtcg cgtttttcgc gggtattcag
    43261 agactcaatc gtttcggtca gcaggcgttg tatttcggtc atcgtttgcc ttcgtggtat
    43321 ctgcaacttt ccagggaaca ccctatttta acgtgggaat agtagaggga aagtaacaga
    43381 tctgccacaa ttttgcataa cagttgcgaa aaaccgatta caatttttta attattctgg
    43441 ctggattacc gcccacgaca acgttgtccg ggacatcttt tgtgacaact gcacctgagg
    43501 ctaccacgac gttatcacca atggtcacac cagggttaat gaccgcgcgt ccgccaatcc
    43561 agacgttatt accgatggtg acgggtttcc ccagttcagc accgctatta cgtgctacag
    43621 ggtcgatggg atgtgttgcc gtgtagatat gaacgcctag tgccaacata cagttatcac
    43681 cgatgcgaat agggcagaca tcaagcatca cgcaatcgaa gttggcgaaa aaattattac
    43741 cgagaaaaat gttatagcca tagtcacagc gaaacgttgg ctcaatataa gcctctgtca
    43801 cctgaccgaa tagatcagcg agaatttgct ggcgtaatgt gtgctcttcc gccagggaat
    43861 gattgtatcg gtgaataagc tgacgagcgc gcaggcgatc gcgagataac gtctcatctg
    43921 ccgagcgata caactcacca gcaatcatct tttctttttc tgtgctcatt actgacctcc
    43981 ggttgctgaa aatgcggcaa cacagtaaga cgaaaagtca gtgaagagaa cgttcccatt
    44041 acatctgtgt gataaagatc acatagggta tgtcttcatg gcgaaaaagt ataaaattct
    44101 taataaacag ccggttatag ctccgaaagc gaattattta ttagcgaata aatttccata
    44161 ctgaggaagg gatcttgtcg tacagtttat tcatggtcaa ttcggcgagg cggtgatctg
    44221 cggctgagta aaataccgcc agttcattat ctgataattc gtatttattt ttctcgataa
    44281 cacgctccag cgtgtcaatt gtctggcaac gacgtaaacg cattaaataa tcggttttcg
    44341 ttaaaggttt ttcggacata cttctaccca tggttgtaat aattctaaca agataaactc
    44401 gcaggattct ctttcgtcgc attgacaaaa caacggaata gtcgattacc tgacttccgc
    44461 catttctgaa gatcctgcat attaatacca taactactga acaacataaa ggtgtcatcc
    44521 agatattcgt cgatctgctc aatgagctta ttgtcttcat tatacttaat tttgtaatta
    44581 agtgcgaagg tcgcaatatg ctcaatcagt tcattcaact ggaggttgat cgccgaggtt
    44641 gggtcgttaa cccagccatg attgctttct tcaaggtttg caaggcagtc atgatacaga
    44701 gtttcacaga gaaacttaag ctgtgcgata tcatgtcttt tgggtgagta ttcatccata
    44761 acgcgtcccc ttcttagcgg ttgaactaac ggacaccttt cgggatggaa aaaacttact
    44821 gacctggact tgccctctcg tttgttagct taactataag ccactctttg caggttttca
    44881 tcgcattatt acgaaaaatt acaattagga aggaagcggg acagggctca ctttttcata
    44941 ctagcaccaa cccgcagcag gtaaaagcag tttgctcgaa ctgttcataa catttcagga
    45001 ttatcgccag atgaaattgg tgacactaat accaggattg ctctgaatat gacgtaataa
    45061 ccgaggaatg aataaagaat taccgcaaat aattaagaat agcctctaaa tgattatg
//
LOCUS       DAZFBR010000025        42216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFBR010000025 DAZFBR010000000
VERSION     DAZFBR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.025"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            121..462
                     /locus_tag="ACHJZ2_001585"
     CDS             121..462
                     /locus_tag="ACHJZ2_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO4567726.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
     gene            complement(453..1379)
                     /locus_tag="ACHJZ2_001586"
     CDS             complement(453..1379)
                     /locus_tag="ACHJZ2_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4567727.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNTLMAGREGDPLYQI"
     gene            1469..2467
                     /locus_tag="ACHJZ2_001587"
     CDS             1469..2467
                     /locus_tag="ACHJZ2_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO4567728.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2464..2682)
                     /locus_tag="ACHJZ2_001588"
     CDS             complement(2464..2682)
                     /locus_tag="ACHJZ2_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO4567729.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2684..4699)
                     /gene="ligA"
                     /locus_tag="ACHJZ2_001589"
     CDS             complement(2684..4699)
                     /gene="ligA"
                     /locus_tag="ACHJZ2_001589"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO4567730.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4770..5768)
                     /gene="zipA"
                     /locus_tag="ACHJZ2_001590"
     CDS             complement(4770..5768)
                     /gene="zipA"
                     /locus_tag="ACHJZ2_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO4567731.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5998..6759
                     /gene="cysZ"
                     /locus_tag="ACHJZ2_001591"
     CDS             5998..6759
                     /gene="cysZ"
                     /locus_tag="ACHJZ2_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO4567732.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6944..7915
                     /gene="cysK"
                     /locus_tag="ACHJZ2_001592"
     CDS             6944..7915
                     /gene="cysK"
                     /locus_tag="ACHJZ2_001592"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO4567733.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8299..8556
                     /gene="ptsH"
                     /locus_tag="ACHJZ2_001593"
     CDS             8299..8556
                     /gene="ptsH"
                     /locus_tag="ACHJZ2_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO4567734.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8601..10328
                     /gene="ptsI"
                     /locus_tag="ACHJZ2_001594"
     CDS             8601..10328
                     /gene="ptsI"
                     /locus_tag="ACHJZ2_001594"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO4567735.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10369..10878
                     /gene="crr"
                     /locus_tag="ACHJZ2_001595"
     CDS             10369..10878
                     /gene="crr"
                     /locus_tag="ACHJZ2_001595"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO4567736.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(10921..11772)
                     /gene="pdxK"
                     /locus_tag="ACHJZ2_001596"
                     /gene_synonym="thiJ"
     CDS             complement(10921..11772)
                     /gene="pdxK"
                     /locus_tag="ACHJZ2_001596"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO4567737.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            11877..12244
                     /locus_tag="ACHJZ2_001597"
                     /pseudo
     CDS             11877..12244
                     /locus_tag="ACHJZ2_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            complement(12247..13158)
                     /gene="cysM"
                     /locus_tag="ACHJZ2_001598"
     CDS             complement(12247..13158)
                     /gene="cysM"
                     /locus_tag="ACHJZ2_001598"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO4567738.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13292..14389)
                     /gene="cysA"
                     /locus_tag="ACHJZ2_001599"
     CDS             complement(13292..14389)
                     /gene="cysA"
                     /locus_tag="ACHJZ2_001599"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO4567739.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            complement(14379..15254)
                     /gene="cysW"
                     /locus_tag="ACHJZ2_001600"
     CDS             complement(14379..15254)
                     /gene="cysW"
                     /locus_tag="ACHJZ2_001600"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO4567740.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(15254..16087)
                     /gene="cysT"
                     /locus_tag="ACHJZ2_001601"
     CDS             complement(15254..16087)
                     /gene="cysT"
                     /locus_tag="ACHJZ2_001601"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO4567741.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16087..17103)
                     /gene="cysP"
                     /locus_tag="ACHJZ2_001602"
     CDS             complement(16087..17103)
                     /gene="cysP"
                     /locus_tag="ACHJZ2_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO4567742.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(17261..18052)
                     /gene="ucpA"
                     /locus_tag="ACHJZ2_001603"
     CDS             complement(17261..18052)
                     /gene="ucpA"
                     /locus_tag="ACHJZ2_001603"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO4567743.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(18181..19038)
                     /gene="murR"
                     /locus_tag="ACHJZ2_001604"
     CDS             complement(18181..19038)
                     /gene="murR"
                     /locus_tag="ACHJZ2_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO4567744.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            19202..20098
                     /gene="murQ"
                     /locus_tag="ACHJZ2_001605"
     CDS             19202..20098
                     /gene="murQ"
                     /locus_tag="ACHJZ2_001605"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO4567745.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            20102..21526
                     /gene="murP"
                     /locus_tag="ACHJZ2_001606"
     CDS             20102..21526
                     /gene="murP"
                     /locus_tag="ACHJZ2_001606"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO4567746.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            21531..22835
                     /gene="pbp4b"
                     /locus_tag="ACHJZ2_001607"
     CDS             21531..22835
                     /gene="pbp4b"
                     /locus_tag="ACHJZ2_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO4567747.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(22893..23792)
                     /gene="yfeX"
                     /locus_tag="ACHJZ2_001608"
     CDS             complement(22893..23792)
                     /gene="yfeX"
                     /locus_tag="ACHJZ2_001608"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO4567748.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(23888..24463)
                     /locus_tag="ACHJZ2_001609"
     CDS             complement(23888..24463)
                     /locus_tag="ACHJZ2_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO4567749.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24524..24973)
                     /locus_tag="ACHJZ2_001610"
     CDS             complement(24524..24973)
                     /locus_tag="ACHJZ2_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO4567750.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(24960..25385)
                     /locus_tag="ACHJZ2_001611"
     CDS             complement(24960..25385)
                     /locus_tag="ACHJZ2_001611"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO4567751.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25599..26468
                     /gene="amiA"
                     /locus_tag="ACHJZ2_001612"
     CDS             25599..26468
                     /gene="amiA"
                     /locus_tag="ACHJZ2_001612"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO4567752.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26472..27371
                     /gene="hemF"
                     /locus_tag="ACHJZ2_001613"
     CDS             26472..27371
                     /gene="hemF"
                     /locus_tag="ACHJZ2_001613"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO4567753.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27377..28429)
                     /gene="eutR"
                     /locus_tag="ACHJZ2_001614"
     CDS             complement(27377..28429)
                     /gene="eutR"
                     /locus_tag="ACHJZ2_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO4567754.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(28475..28975)
                     /gene="eutK"
                     /locus_tag="ACHJZ2_001615"
     CDS             complement(28475..28975)
                     /gene="eutK"
                     /locus_tag="ACHJZ2_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO4567755.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(28988..29647)
                     /gene="eutL"
                     /locus_tag="ACHJZ2_001616"
     CDS             complement(28988..29647)
                     /gene="eutL"
                     /locus_tag="ACHJZ2_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO4567756.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(29657..30544)
                     /gene="eutC"
                     /locus_tag="ACHJZ2_001617"
     CDS             complement(29657..30544)
                     /gene="eutC"
                     /locus_tag="ACHJZ2_001617"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO4567757.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(30565..31926)
                     /gene="eutB"
                     /locus_tag="ACHJZ2_001618"
     CDS             complement(30565..31926)
                     /gene="eutB"
                     /locus_tag="ACHJZ2_001618"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO4567758.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(31938..33341)
                     /gene="eutA"
                     /locus_tag="ACHJZ2_001619"
                     /pseudo
     CDS             complement(31938..33341)
                     /gene="eutA"
                     /locus_tag="ACHJZ2_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
     gene            complement(33338..34564)
                     /gene="eutH"
                     /locus_tag="ACHJZ2_001620"
     CDS             complement(33338..34564)
                     /gene="eutH"
                     /locus_tag="ACHJZ2_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO4567759.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAADMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(34664..35851)
                     /gene="eutG"
                     /locus_tag="ACHJZ2_001621"
     CDS             complement(34664..35851)
                     /gene="eutG"
                     /locus_tag="ACHJZ2_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO4567760.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            complement(35841..36677)
                     /gene="eutJ"
                     /locus_tag="ACHJZ2_001622"
     CDS             complement(35841..36677)
                     /gene="eutJ"
                     /locus_tag="ACHJZ2_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO4567761.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(36688..38091)
                     /locus_tag="ACHJZ2_001623"
     CDS             complement(36688..38091)
                     /locus_tag="ACHJZ2_001623"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO4567762.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(38103..38373)
                     /gene="eutN"
                     /locus_tag="ACHJZ2_001624"
                     /pseudo
     CDS             complement(38103..38373)
                     /gene="eutN"
                     /locus_tag="ACHJZ2_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
     gene            complement(38480..38773)
                     /gene="eutM"
                     /locus_tag="ACHJZ2_001625"
     CDS             complement(38480..38773)
                     /gene="eutM"
                     /locus_tag="ACHJZ2_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO4567763.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(38812..39828)
                     /gene="pta"
                     /locus_tag="ACHJZ2_001626"
     CDS             complement(38812..39828)
                     /gene="pta"
                     /locus_tag="ACHJZ2_001626"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4567764.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(39825..40556)
                     /gene="eutT"
                     /locus_tag="ACHJZ2_001627"
     CDS             complement(39825..40556)
                     /gene="eutT"
                     /locus_tag="ACHJZ2_001627"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO4567765.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(40553..41254)
                     /gene="eutQ"
                     /locus_tag="ACHJZ2_001628"
     CDS             complement(40553..41254)
                     /gene="eutQ"
                     /locus_tag="ACHJZ2_001628"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO4567766.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(41229..41708)
                     /gene="eutP"
                     /locus_tag="ACHJZ2_001629"
     CDS             complement(41229..41708)
                     /gene="eutP"
                     /locus_tag="ACHJZ2_001629"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO4567767.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(41721..42056)
                     /gene="eutS"
                     /locus_tag="ACHJZ2_001630"
     CDS             complement(41721..42056)
                     /gene="eutS"
                     /locus_tag="ACHJZ2_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO4567768.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT         9745 a        11840 c        10788 g         9843 t
ORIGIN      
        1 aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg cgatttgtaa gattgatttt
       61 tcttctcaag ttccttgttc ttctgccgat attacctcgt acagtgacga ggactgaaca
      121 atggccacta tctcttcaac ttcaattcct tccatacaaa cacagagtag taacagggca
      181 tcacaaggta gcgatgttgc ttcacaaatc gcgcggataa gtcagcaaat catcaaactc
      241 actcaacaca ttaaagagat tgtagacact agcggcagtg ctgaagacaa gcaaaaacag
      301 gccgaattga ttcaacagca aatcacccta ctggaaacac aactggcgca gttgcagaga
      361 cagcaggcag aaaaggcgca ggagaaagag cagcgtttgt ctctgaatgt atcccttcta
      421 aatcctgttg aaaatacgac tcatatcggt atctatatct gatacaacgg atccccttcc
      481 cgcccagcca ttagcgtatt gccctggtct cgtaccacgt cccataacgc ttcagcggcg
      541 gtggaaagtg aacggttttt cctacgcacc agcatcaact ggcgttccac caccggcgta
      601 atgcgcttta ccaccagcgg gctaccttca ggcaatggta gcgccagtgc gggaagaata
      661 ctgatgccga tgcctgcggc taccatcggg aacagcgtcg ccggatgtcc aatctcctgc
      721 acaatattcg cctgaatacc attgcgcgcc agcgctgcgt caatcagcgg tcggctgcct
      781 gacgcataat cctgcaacac caatttcgct ccctgtaatg cctgccaggg tacataatct
      841 tccgcggcca aagcgctatc ccgatggcaa agcagaaaga aaggctcgga aagaatcact
      901 tcacattgca gatcgcccac tgggcctgga tcgatgacga tgccaaagtc gacatctccc
      961 tggcgaatac tttccatcac ccactgctgc ggacgatcgt gcaggacaaa ctgaatatct
     1021 ggatagcggc gatggctttc agcaatgcat tgcggaataa gatgcgcgga aatggtctgg
     1081 ctggcagcga cccgaacttt tccgctcagt tgttgcccca tacgcccggt atcgcgcaac
     1141 gtgctgttaa gttcatccag cagtcgctca agacgcaaag ccaactgctg ccctgcatct
     1201 gtaagcacca cttcacgcgt ggttctgtcc agcaggcgaa caccagtatg attttccagc
     1261 tccttcacac tgtgacttac tgccgactgg ctcaggccga tacgctctcc tgcacgacta
     1321 aaacttttct cctgcgctac tgtgacgaaa acctttaatt gttttaaaga ataattcatc
     1381 tgttttcttc atgaatggat gcaataaatc aattttattt ctcaaactgg aagaagcaca
     1441 atagaaccat cgatcatctg gagtctttat gaaacttttt cgtatcctcg atcctttcac
     1501 cttaaccctg atcacggtgg tgttgctggc ctctttcttt cctgccagag gcgatttcgt
     1561 ccccttcttt gaaaatctga ccaccgcagc tattgccctg ctgttcttta tgcacggcgc
     1621 gaagttgtcg cgtgaggcga ttattgctgg cggtggtcac tggcgactgc atttgtgggt
     1681 aatgtgcagc accttcgtgc tgtttccgat tctgggtgta ctgtttgcct ggtggaaacc
     1741 ggtaaatgtc gacccgatgc tctactccgg ttttctctac ttgtgcattc tcccggctac
     1801 cgtgcagtct gcaatcgcct tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg
     1861 ttctgcgtca gcatccagtc tgctggggat tttcctttca ccattgctgg ttggtctggt
     1921 gatgaatgtt cacggtgcag ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact
     1981 gctgctgccg tttgtgttgg ggcatctttc ccggccgtgg attggtgact gggtgtcgcg
     2041 caataaaaaa tggattgcga aaactgacca gacgtccatt ctgttggtgg tttatacagc
     2101 gttcagcgaa gccgtcgtta atggtatctg gcataaagtt ggctggggat cattgctgtt
     2161 tatcgtggtg gtcagctgcg ttcttctggc tatcgtgatt gtagttaacg tctttatggc
     2221 acgccgactg ggcttcaata aggcagatga aattactatc gtcttttgtg gttcgaaaaa
     2281 gagtctggca aatggcatcc cgatggcaaa cattctgttc cccacatcgg tgatcggtat
     2341 gatggtgctg cccctgatga ttttccatca gatccaattg atggtctgtg cggtgctggc
     2401 gcgtcgatac aaacgccaga ccgaacagtt acaggcgcag caggaaagca gcgccgataa
     2461 agcttaaagc ggacgcttca ggggctggac cagttgcgtc agcccctcgg ttttaatcag
     2521 cagcgtgatt tgcattagct caccgagctt ccctgccggg aactcatctt tgcgggcaaa
     2581 ccacagcaga tactcctccg gcaggtcgat taagcgacgc cctttgtatt tgccaaacgg
     2641 cattatcgta ttggctattt caatcagctg ctctttttcc atctcagcta cccagcaaac
     2701 gcagcatttc cgcttcgtcg atgacttcaa tgcccagttc ctgcgccttc gccagtttag
     2761 atcctgcagc ttcacccgct atcaccagat cggttttctt cgacacgctg cccgcgactt
     2821 tcgcgcccag ttcgaccagt cgagctttag cgtcatcacg cgacatctgg cttaagctgc
     2881 ccgtaagcac cacggtttta ccagcaaacg ggctgtcaat ctcttccgcg ttgataacga
     2941 tcggcgcagg ccagtgaaca ccttccgcca acagctcgct gatgacattg cggttgcttt
     3001 cttcggcaaa gaagttgtga acgtgggatg caacgacaat gccaacatca ggcacctttt
     3061 gcagctcttc aatcgaagcg gcttccagcg cttccagcgt tccgaaatat gccgccagac
     3121 ctgctgcggt ggcctcgccg acttcacgga tgccaagtgc atagaggaag cgagcaaagg
     3181 tggtttcttt cgctttttcc agcgcgttaa ccacgttttg tgccgatttt ggccccatac
     3241 gctccagtcc ggtcagtttg cctgcggtga gtttgaacag atccgccgga gtgtggacat
     3301 attctttttc aaccagctga tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac
     3361 ggcgggaaac aaagtgtttc agcgactctt tacgctgcgc accgcaaatc agaccaccag
     3421 tacagcgggc aaccgcttca ccttccacac gctcaacgtc agaaccacat accggacaat
     3481 acgtcgggaa tacaacctca cgggtatctt ccgggcgttc agaaagcacg acgttaacca
     3541 cctgcgggat cacgtcgcca gcgcggcgaa tcaccacttt atcgccaatg cgtaaaccaa
     3601 gacgttcgat ttcatccgca ttgtgtaagg ttgcgttact caccagcacg cctgcaacat
     3661 ggacaggttc cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga
     3721 cgtcacgcac aaaggtcatc tgttcctgcg ccgggaattt aaacgctacc gcccagcgcg
     3781 gggctcgcgc gacaaagcca agctgctcct gctgttccag tgagttgacc ttaatcacca
     3841 cgccgtcgat atcaaagccc agcgtcgggc ggtcttcttc cactttgtga tagaacgcca
     3901 gcacttcttc cgccgattca caaagcgtta cccgatcgct gaccggcaac ccccactttt
     3961 taaattgcag taaacggcca agatgagtat ccggcagctc gccaccttcc agaacaccaa
     4021 cgccatagca gaaaaaagtg agcggtcgct tcgctgtaat acgcggatca agctgacgca
     4081 gtgaaccagc tgccgcatta cgtgggttag caaacacttt cccgcccgtg cgtcgcgcat
     4141 cttcgttaat cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc acttccagac
     4201 gcgccgggat attctctccg tgcaacttca gtggaatggc gcgaatagta cgcacattag
     4261 acgtgatatc ttccccggtg gtgccatcgc cacgagtcgc ggcactgact aaaacgccat
     4321 tttcatacag aatactgacg gcaaggccat ccagcttcag ctcacagcac caggtgactt
     4381 tctcgttgct tttcagacgg tcctgcacac gtttgttgaa ggcaagaaag ctttcttcat
     4441 caaaaacgtt atccagcgac agcattggca cttcatggcg aatctggctg aaagccgcca
     4501 gcggcgcagc acctacacgt tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg
     4561 tttccagctc gcgcagttcg cgcatcagcc tgtcgtattc agcgtcggga atttccggcg
     4621 catccatcac atgataaaga tattcatgat ggcgaagcgt cgttcgcagt tctgtcagtt
     4681 gttgttcgat tgattccata tcgcaccatc aatgctaaaa acccccgaca agcgggggtt
     4741 cgaagaggag ttaatttgcc ttaagtgtat caggcgttgg cgtctttaac ttcgcggatg
     4801 atgtcctggt actcgcgcaa tttctgcgga gtcatcatac ggcgctgatc gtcaagcacg
     4861 acaccgccca cttcatcggc aatatgctgc gcagattgca gcatcagctt gaagttctgc
     4921 agctcgtcac cgtaagacgg tacctgcata aagatagtga cacccggagt agtgaaatcc
     4981 ttcatttcag gatcaaaggt tcccggtttc accatattcg ccaggctaaa taacgccggg
     5041 ccgctgccat ccgggctaag atgacgatgg taaatattca tatcgccaaa aatgaagccc
     5101 gcttgttgaa tgctgttaag aagcagttca ccgtttagct cgctaccgtg atgcgccgcg
     5161 acgttcatga taatcaccgc ttctttgcgc ttcggtttat ccataactgg agccggttcc
     5221 gctacaggct caggctgtgg tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc
     5281 ggttgcggtg ctgaatgcac tggctgcggc gctggcgcga cctgtggcga aactggctgc
     5341 tgcaacggct gttcaggctg cggctgatag gcaggctgct gcacgggttg ctgcaccggc
     5401 tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg ctggaccggt
     5461 tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca
     5521 gcctcatgct cctgggcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca
     5581 acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt
     5641 cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca
     5701 tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc
     5761 tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca tttactcact
     5821 aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat
     5881 agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt
     5941 acccggtaaa atacgcacaa ttttcctggg attggtcaaa aggagctcat cctgattatg
     6001 gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag
     6061 ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg
     6121 ttgatggggg gcgcattctg gtggctcttt acgcaactcg atgtctggat cccgactctc
     6181 atgagttatg ttccggactg gttacaatgg ctgagctatc tgttgtggcc tctggctgtc
     6241 atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct
     6301 ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca
     6361 gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa
     6421 aaatttgcct ggtatctgcc acgcgcaatt gtattgctaa ttctttactt catccctggt
     6481 attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc
     6541 cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc
     6601 ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagtct gtttacgatg
     6661 atcccgctgc ttaatctgtt cattatgccc gttgccgttt gtggcgcgac ggcgatgtgg
     6721 gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta
     6781 aaccgtttgt gtgaaacagg gggggcttat gccgcccctt attccatctt gcatgtcatt
     6841 atttcccttc tgcatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta
     6901 tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga
     6961 agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg
     7021 acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg
     7081 tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt
     7141 tgaaccaacc agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta
     7201 caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc
     7261 gttaggtgca aacctggtgc tgaccgaagg tgctaaaggc atgaaaggcg caatccaaaa
     7321 agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa
     7381 tccggcaaac cctgaaattc acgaaaagac caccggtccg gagatctggg aagataccga
     7441 cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag
     7501 ccgctacatt aaaggcacca aaggtaagac cgatcttatc tctgtcgccg ttgagccaac
     7561 cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg gcccgcataa
     7621 aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc tggtcgataa
     7681 agtcattggc atcaccaatg aagaagcgat ttctaccgcg cgtcgtctga tggaagaaga
     7741 aggtattctt gcaggtatct cttctggagc agctgttgct gcggcgttga aactacaaga
     7801 agatgaaagc tttaccaaca agaatattgt ggttattcta ccgtcatcgg gtgagcgtta
     7861 tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac agtaatgcca
     7921 gcttgttaaa aatgcgtaaa aaagcacctt ttcaggtgct tttttgtggc ctgcttcaaa
     7981 ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta
     8041 ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt
     8101 ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac
     8161 acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa aaactggcgc
     8221 taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct
     8281 ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc gaacggtctg
     8341 cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact
     8401 gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc
     8461 ctgactcaag gtacagttgt gactatctcc gcagaaggcg aagacgagca gaaagcggtt
     8521 gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca
     8581 gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg
     8641 gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg
     8701 accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc
     8761 tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg
     8821 aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta
     8881 aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg
     8941 ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg
     9001 gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg
     9061 atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga
     9121 agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg
     9181 cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa
     9241 atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg
     9301 aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg
     9361 ctaaactgaa agatctgcca gctattacgc tggacggtca ccaagtagaa gtatgcgcta
     9421 acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc
     9481 tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa gaagaacagt
     9541 ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca
     9601 tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt
     9661 tcctcggctg gcgcgctatc cgtatcgcaa tggatcgtaa agagatcctg cgcgatcagc
     9721 tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca
     9781 tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc
     9841 gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg
     9901 ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca
     9961 atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt
    10021 accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg
    10081 ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc
    10141 tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta
    10201 agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg
    10261 ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa
    10321 tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc
    10381 gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat tgagatcatt
    10441 gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa
    10501 aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta
    10561 gacggcacca ttggtaaaat ctttgaaacc aaccacgcat tctctatcga atctgatagc
    10621 ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc
    10681 aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat
    10741 ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg
    10801 gacgaaatca aagaactgat caaactgtcc ggtagcgtga ctgtgggtga aaccccggtt
    10861 atccgcatca agaagtaatt cttgctgcag tgaaaaatgg cgcccatcgg cgccattttt
    10921 ttatgcttcc gccagcggcg gcaaaatcaa ttcatcgctc tcatgctgct gggtgtagcg
    10981 catcacttcc agtacgcgca accccgcgcg gtgcaccgca tcgtttaatg ccttcccttt
    11041 cagcaaacca ctgataagct gagcacaaaa caggtcgcca gtccctttca ggtcggtttt
    11101 tacccttgaa tgggaaatga cattcacgct gtcggcagtg accaccacaa ccagcatctc
    11161 ctgattttct tcattaccgg aggcgctggt aatcaccacc cattttagtg tgtctgaaag
    11221 cagacttttt gcggcagcaa tggcactgtc gagatcgcgg caatttttac cggtcaggat
    11281 ttccaactca aagatattgg gggtaattcc ctgcgccagc ggcagtaaat attgtcgata
    11341 cgcttcggga aggtcaggtt tgacataaat tccgctgtca atatcgccaa tcaccggatc
    11401 gaccatgatc aataggtcag gatggtcttt gcgtagcgca gtcagccact cggcaaggat
    11461 tttaatttgc gatgccgttc ccatatagcc cgtggttaca gcacgaagtt ggcgcagcgc
    11521 atcacgctcc tgaagtgcac gcaaatagcc gctaaaccat tcatccggaa tcgcaccacc
    11581 gtagaaagtg tcataatgcg gcgtattgct cagcaatacc gtcggcacgg caaagacatt
    11641 caggccgttc tgtttgatag caggcacggc aatgctgttg cccacgctgc cgtaaaccac
    11701 ctgcgactgc acggcgacga tatccgcctg cagcgccctg ctcttatcgt taaacaacaa
    11761 caaactactc atttaatttt ttctccttgc cgatgatcct catcgtattc caaccgaaac
    11821 tttacctgat tctggcagtc aaatcggcta ttacaaaaca aggataaggt aattcagtga
    11881 agaaaatcat ttgtctggtc attacactac taatgacact ccccgcttac gcgaagttaa
    11941 ctgcccatga agaagcccgc atcaacgcca tgctggaggg attagcacag aaaaaggatt
    12001 tgatatttgt gcgcaacggt gatgaacata cctgcgatga agcggtttct catctgcgtc
    12061 tgaagctggg caatacccgt aaccgcattg acactgccga gcagtttatt gataaggttg
    12121 cttcgtcgtc atcgattacc gggaagccgt atattgtgaa gatccccggt aagagcgatg
    12181 agaatgcaca gcctttttac atgcgttaat tgcgcagacg gataaaacgg tgcctgcgca
    12241 ataatcttaa atccccgccc cctgactaaa atgctcttcc ccaaacaccc cggtggaaag
    12301 gtagcgatcg ccacgatcgc agatgatcgc taccaccacc gcaccagggt tagcttttgc
    12361 cacccgcagt gctccggcaa ccgcgccgcc ggagctgacg ccacagaata ttccttcccg
    12421 caccgccagt tcgcgcatgg tgttttccgc atcgcgctga tgaatatcca gcacctcatc
    12481 taccagagaa gcgttgaaaa tccccggcag atattccgca ggccagcggc gaatgccggg
    12541 aatgctactg ccctcttccg gctgcaggcc aacaatggtc accggtttgg attgttcgcg
    12601 cataaagcgt gaaacgccgg tgatagtgcc ggttgttccc atgctggaga caaaataggt
    12661 aatgcgcccg ccagtctgct gccagatttc cggcccggta gtggtgtaat gggcgtaagg
    12721 gttatcggga ttattgaact gatcgagcag ctttccttca ccacgatccg ccatctccag
    12781 cgccagatcg cgcgcacctt ccatgccctg ctctttggtg accagaatca gttccgcacc
    12841 ataagcacgc atcgccgcac ggcgttcctg gctcatgttg tcgggcatca gcaatttcat
    12901 gcgatagcct ttaagcgcgg caatcattgc cagcgcaatg ccggtgttac cactggtggc
    12961 ttcgattaac acatcacccg gtttaatttc cccgcgcttt tccgcctcga cgatcatcga
    13021 aagcgccgca cgatctttta ccgaacctgc cgggttattg ccttccagtt ttaaccacac
    13081 ttcactgccg ttattcggcc ccattcgctg caacttcacc agaggcgtat tgcctattgt
    13141 ttgttctaat gtactcacga tctctatcca tacgtggtgt tgcctgatgc gacgcttgcg
    13201 cgtcttatca ggcctacagg ttacaaacct tgccattaaa aagcccggat cgcgggaagc
    13261 gcctccgggc gtttaacatt cactcaacct atcaggcgct ttgtgcgaga gcaagttcct
    13321 catctcgggg ttcgatacgc tcgtcgccgt tatacagccg cgcatgttgc agaccaacga
    13381 ataaacgctc gccacgctgc ggggcatcgt cgccatgcat cacgaccgtc agcggttcgt
    13441 tgtaccaccc cagcggctgc accactaatt gggtgtagtg acctttcggg ctggcttcca
    13501 gtacctgtac cggcagcggc gaatcgaggc tggtacggcg gctgatatcc acttcccacg
    13561 ggcgcaggaa gagatccacc ggcccctgat acgcaggtgt gtagcccagc ggccagcgat
    13621 gtgcgccaac atggaactgc ccgccgcgaa tggttccctg caggcggttc acttcgccca
    13681 taaattcgag cacaaaacgg gtcgccggtt cgcgccatac ctgatccggc gcgtcagcct
    13741 gttcaatatt gccctggctc atcacaacta cacgatcagc tacttcggtc gcttcttcct
    13801 gatcgtgggt cacaaaaacg ctggtgaatt ttagttcttc atggagttga cgcagccatc
    13861 gacgcagctc tttacgcacc tgcgcatcca gcgcgccaaa cggttcatca agcagcagaa
    13921 tttgcggttc tacagcaagc gcacgcgcca gcgctacacg ctgtttctgg ccgccggaaa
    13981 gctgcgccgg ataacgatcc gccagatggg caagctggac catttccagc aattttgtca
    14041 ctttcgcttt gatggctgcg gcattcgggc gctcgcgacg cggcagcacc gtcaggccaa
    14101 aagcgatatt gtcgaacacc gtcatatggc ggaacagcgc gtaatgctgg aacacgaaac
    14161 cgactttacg atcacgtgcg tgcaggcggc tcacgtcggt gccgtggaag cgaatatgcc
    14221 cgctggtttg atgctccagc ccagcgataa tgcgcagcag cgtggttttc ccggaaccgg
    14281 acggccccag caacgcgacc atctgacctg aaggaatatc cagtgagata tcgttcagca
    14341 cctgggtgcg accaaacgac ttcttaatat tggcaatctc aatgctcatg attttcctcc
    14401 tgctgcgcgc gtttttcctg attctccagg cgccactgca acatactctt taaaaacagg
    14461 gtgataatcg ccatcagcgt caacagcgcg gcggcggtaa aggagccgac tgtgttgtag
    14521 tcctgctcca gcaattcaat ctgtaacggc agcgaaagtg tttcgccacg aatcgagccg
    14581 gaaaccaccg acaccgcgcc aaactcgcca atcgcgcggg cgttggtcag cactacgcca
    14641 taaagcagcg cccagcggat gttcggcaat gtgacacggc ggaacatctg ccagccggat
    14701 gcgccaagca aaatcgccgc ttcgtcttcc tgactgccct ggcttaacat caccggcacc
    14761 agttcgcgca ccacaaacgg acacgtcacg aagatcgtga ccagcaccat tccgggccag
    14821 gagaacataa tttgcagatt atgttcatcc agccagccgc cgagcgggcc gttggagccg
    14881 tagaacagca gatacaccag accggcaacc accggtgaca cggcaaaagg aatgtccagt
    14941 agcgtcagca gtaactggcg tccggggaag ttaaagcgcg tcaccagcca ggccagcaga
    15001 ataccgaaca ccaggtttac cggtacggca atcagcgcga tcatcaccgt cagccagatg
    15061 gcgtgcagca tatccggatc ggccagattc tgtaaaaccg gcatcagccc tttgctgaac
    15121 gcctgtacga agatataaat catcggcacc agcaggatga acgccgatac cagcatcccg
    15181 atgccaatca gaaaccattt gccccagtta atcgggcgcg cgtcataacg cttcaattgt
    15241 gtcacttccg ccattaatga cctaccacac gccgaccaaa gcgactttgc agagtgttaa
    15301 ttgagaacag cagcagcaga gatgccgcga ggatcaccga agcaatcgcg ctcgccgccg
    15361 ggtaatcaaa ctcctgtaag cgaacgaaaa tcatcagcga cgtcacttcc gtcttccaag
    15421 cgatgttccc ggcgataaat atcaccgcgc caaactcacc aagactacgg gtaaacgaca
    15481 acgccacgcc cgccaccagc gccggagaaa gttccggcag caccactttg cagaaactct
    15541 gccagcgcgt tgcgccaagc gtttctgccg cttcttcata ttccgggcct aactcttcca
    15601 gcaccggctg cacggtacgt accacaaacg gaatgctggt aaaggccatt gccaccgcaa
    15661 tccccagcca ggtataggtg actttgatat caaacttcgc cagccattca ccgtaaaaac
    15721 cgtttacaga aaagagcgag gccagcgtta aaccggcgac tgccgttggc agcgcaaagg
    15781 gtaaatccat cagcgcatca agcagcgtgc ggcccggaaa gcgataacgg gttaaaatcc
    15841 acgccatcag caggccgaaa acgccgttaa aaattgatgc cacaaacgcc gacagcagcg
    15901 tcactttgta ggccgcgacc acctgcgggt tggtgatcac ttcccagtac tgcgcccagc
    15961 tcatctcaga aagctgcatc accagcgcgg agagtggcag cagcaatatc aggcacacaa
    16021 acagcagact ggtgccgagg cttaaggtaa agcccggcag gacgcgtctt gatgaaactg
    16081 caaacattaa ttacgccccg ccgctaacag cttgtctaac tcgccgccgc tggtaaagtg
    16141 ggttttcatc acttccggcc aggagccaaa tttgtcttcc acgcggaaca gttcggtctg
    16201 cgggaattta tctttcagtt tgtccatgac ttccgggtta ttcacgcggt agtaatagtc
    16261 ggtgatgatg gtttgcgcct gcgggctgta aagccagttc agataggctt ttgctgcttt
    16321 ttcggtaccg ttggcttgca cgtttttatc gacccacgcc accgggaatt ccgccagaat
    16381 gttggttttc ggaatcacca cttcaaagcc ctgcgcttcg tactgtttgc ggatgttgtt
    16441 cacttccgat tcaaagctaa tcagtacatc gcccaggccg cgctcggcaa aggtggtggt
    16501 cgcgccacga ccgccggtat cgaacacttc aacgtttttc aggaactgag tcataaactg
    16561 ttcggttttg gctttgtcgc caccgtcagc tttatccgct gcgccccatg ccgccagata
    16621 ggtataacgc gcgttacccg acgttttcgg gttcgggaaa atcagcttca cgtcggagcg
    16681 caccaggtcg ttccagtcgt ggatattctt cgggttgccc ttacggacca ggaagcccat
    16741 ggtggagtag aacggcgagc tgttattcgg caggcgcgac tgccagtccg ccgggataag
    16801 cttgccttta tcgtgcagaa tttgtacgtc ggtcacctgg ttataagtga caacgtcggc
    16861 ttttaagccc tgcaaaattg ccagcgcctg ttttgatgac ccggcatgag attgttttat
    16921 cgtcagtttg tcgccgccgt tatctttggc ccattgctgt tcaaacggcg ggttcagggc
    16981 ggcaaacagc tcgcgggaga cgtcataaga actgttcagc agttccgttg cctgtacatg
    17041 gcccgccagc agcagagaag cgaccagcgc gagtgagttc tttttcagta agttaacggc
    17101 cattgcgcac ccttataaat ttaatgactt tctaatagcc atcatattta taacggacgt
    17161 gaaaggagta acggttttat ataccgtttg gtgatttgga agttgaaaag ggaataagac
    17221 gaaatcctcc ccacaaatgc agggaggaaa cagaggtgaa tcagataccg acgctaaccg
    17281 tctccggcag tgtgctgccg ccatcaatca cattctgtgt accggttaaa tagctggatt
    17341 catccgatgc gaggaaggcc gccagttcgc cgacttccag cggatcggcg aggcgacgca
    17401 gcgggattgc ttttgccatt tcagtcagca ccgactctgg atcttccggg ttcgactggc
    17461 gggcaatgct ttccgccatt ggtgtgcgaa cgtatccagg gcatatggcg ttaacgcgaa
    17521 taccagactg cgcatactcc accgccagcg attttgtcag gccaacaatc gccgctttcg
    17581 ttaaggcgta cgccgtttca cccggatcgg ccaccatatc accagtgact gaagacatca
    17641 tcacaatgcg accatctttg cgggcaatca tctccggcag caccgccttc gtgacgttcc
    17701 atacgccttt aatattgatg tcaatatgga aatcgcgatc gtcatcgctc atatcgagga
    17761 aactgcccag acgacaaacg cctgcgttat tcaccaggat atcaatgcgc ccttcttttt
    17821 ccttcgcgcg tttgatagct gcggctaccg acgccgggtc acgcacatcg gcgacaaccg
    17881 ccgtacagcg atgaccacga ccacacagtt cgtccgccag cttttcgatc tcaggggaga
    17941 tatccagcaa gattaggttt gcgccatgac gcgcaaaagt tctggcaatt ccttcgccaa
    18001 ttccctgcaa tgcgcccgta atcagtgctg tcttgcccgt gagtttaccc attttaatgc
    18061 tctccttgtt aatacagagc gttacactcc attccctctg aaaatacagg ataaacaaaa
    18121 tattactgcg gatcagctca ctaattcagg aaaatctcaa tccagtcgct gctttatgca
    18181 tcatttcagg cgttgtgtta gctcactgct gcgctgaatc attttcaatg actccacatc
    18241 attgagttgc acaagaccaa caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt
    18301 agacatcgac gagctacgcc attcggtttc accggaaacg gtatcgagcg tgaaatgcgc
    18361 cagccgccgc agcggagagt cagccagaga agtaatggca atcaccgttg ccccctgctt
    18421 tcgtgccgct tctgcgcaca agacaatctc ttttttactg ccgctgtaag agatagcgat
    18481 ttgtacatcg ccttttttca acgcctggga aacggtggct tgtacgtgcg tgtcggcttc
    18541 gcaggccacg cgatatccaa ttttcatcag tttgaacgac agatcgcacc ccaccagcgc
    18601 cgatccgccc aggcccgtta tctggataaa cggtgcttta ctgatgacct caatgatttt
    18661 ttgcagccgc gcgtagtcga acaacgcaca ggtttgctcc agcgccagct ctttttcgcg
    18721 attcaatttg cgggcaatca cttccagcga atcatcactg gtaatcgaac tgtgcaggtg
    18781 ctgcgccgtg gcattcgttt tctcccggct ggcactgtat tcgcctatta acgccatacg
    18841 caattcagta aacccttgtg cgccgagctt ctgggcaaac ttcacaatac tcgactggct
    18901 gatgccgagc tgtttcgcca tcttgcgcga agagacggat ttcagctcac tcacgttggc
    18961 ccgcagaaag tcagcaattt tttgctcgtt ctcggtaaat tcactttccg cattgcgtat
    19021 tttcgtcagg tacaacatct tccactctcc tgggctggca gcaaaaattc cgcttaagtt
    19081 cttatgacgc tcttcacact ctgcgagtgt agactcaatg attcctttaa gtctgtcaat
    19141 tcgggatata tgattccata catctgcaat tatttgatcg taaatagtaa ggtcaccacc
    19201 gatgcaactt gaaaagatga ttaccgaagg ctcgaacgcc gcctcggctg aaattgaccg
    19261 cgtatcaacg ctggaaatgt gccggattat caacgatgaa gataaaaccg taccgcttgc
    19321 cgttgagcgc gtactgccgg atatcgccgc ggcaatcgat gttatccacg cccaggtaag
    19381 cggcggcggt cgtctgattt atctcggtgc gggaacatcc ggtcgtctgg ggattctgga
    19441 tgccagcgaa tgtccgccca cctacggcgt gaaaccgggt ctggtagttg gattaattgc
    19501 tggcggcgaa tatgccattc agcacgcggt ggaaggcgct gaagatagcc gggaaggcgg
    19561 tgttaatgat ttgaaaaaca tcggattaac cgcgcaggat gtggtggttg gcattgctgc
    19621 cagcggtcgc acgccgtatg tgattgccgg actggaatat gcacgccagc tcggctgccg
    19681 tacagtggga atttcctgta atccggggag cgccgtttcc accaccgctg agtttgctat
    19741 tacgccggtg gttggggctg aagtggtcac cggttcttcg cggatgaaag caggcacggc
    19801 gcagaaactg gtgctcaata tgctttccac cgggctgatg attaaatccg gcaaagtgtt
    19861 cggcaacctg atggtcgatg tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa
    19921 tatcgttaaa aacgccaccg gatgtaatgc cgaacaagca gaagcagcgt taattgcctg
    19981 cgagcgcaac tgtaaaacgg ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc
    20041 taaaaaacgc ctggatcaac acggcggctt tattcgtcag gttttagaca aggaataacc
    20101 catggccaaa gagatcagca gtgaacttct gaacaccatt cttacccgtg tcggcggacc
    20161 gggaaatatc gccagttgtg gtaactgtat gacgcgcctg cgtctgggtg tacatgacag
    20221 ttcactggtt gatcccgata tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag
    20281 tgaccaggtg caggtcgttt ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag
    20341 cgagctgctg ggagaagcac cggtacagga tgccgcagaa atcgccgccc agaacaaacg
    20401 tcagttaaaa gccaaacaaa cctccggcgt gcaacaattt ctcgccaaat tcgccaccat
    20461 cttcacgccg cttatccccg gttttattgc cgccggtctg ctgctgggga tagcgacgtt
    20521 aattgccacg gtgatgcacg ttccggcaga tgctcagggc acgctacccg acgcgctgaa
    20581 ttttatgaag gtgttcagca aaggtttgtt caccttcctg gtgattctgg tgggctataa
    20641 cgccgcccag gcatttggcg gcacgggcgt caatggcgca attattgccg cactcttttt
    20701 gctcggttat aaccccgctg caaccaccgg ttactacgcc ggttttcacg atttctttgg
    20761 tctgcccatc gatccgcgcg gcaatattat cggcgtgttg attgccgcgt gggcctgcgc
    20821 ccgcatcgaa ggcatggtgc gccgctttat gcccgacgat ctcgacatgc tgctgacctc
    20881 gttaatcacc ctactgatca ccgctacgct ggcctatctg atcattatgc cgctgggcgg
    20941 ctggttattc gaaggtatgt cgtggctgtt tatgcacctg aacagtaatc cgctcggttg
    21001 tgcggtttta gccgggctgt tcctgatcgc cgtggtattt ggcgtgcatc agggctttat
    21061 tcctgtttac ctcgcgttaa tggacagcca aggattcaac agcttatttc ccatcctttc
    21121 gatggcaggc gcgggccagg tgggcgcggc gctggcactc tactggcggg cgcaaccgca
    21181 cagtgcatta cgcagtcagg tacgcggggc gattattccc ggcctgctgg gcgttggcga
    21241 accgttgatt tatggcgtca ccctgccccg catgaaaccg tttattaccg cctgtttagg
    21301 cggcgcggcg ggcggtttgt ttatcggatt gatagcctgg tggggtctgc cgatgggctt
    21361 aaacagcgcc tttgggccgt ctggtctggt ggcgctgccg ctgatgactt cagcacaagg
    21421 cattcttccg gcgatggcag tctacgctgg cgggattctg gtggcatggg tttgcgggtt
    21481 tattttcacc acgctttttg gctgccgtaa cgtcaatctg gactgatatc atgaaacgga
    21541 caatgctcta tctttctctg ctggctgtta gttgtagcgt cagcgccgca aaataccctg
    21601 ttctgacaga aagttcgcca gagaaagcag ggttcaacgt cgaacggctt aaccagatgg
    21661 atcgctggat tagccagcaa gtggacgccg gttatcccgg cgtaaacctg ctgattataa
    21721 aagataatca gattgtgtat cgcaaagcct ggggagcggc gaaaaagtac gatggcagcg
    21781 tattgatggc gcagcctgta aaagctacca ccgggacgct gtatgacctg gcctcaaaca
    21841 ccaaaatgta tgccactaac ttcgctctgc aaaagctaat gagcgaaggc aaactgcatc
    21901 ctgacgatct gattgcgaaa tatatttcgg ggtttgccga tagtccaaat gacaccatca
    21961 aaggcaaaaa cacgctgcgg atttctgacc tgctgcatca cagcggcggt ttcccggcgg
    22021 acccacaata tccgaataaa gcggtcgcgg gcgcgttata ttcccaggat aaaggccaga
    22081 cgctggagat gatcaagcgc acgccgctag agtatcagcc cggcagcaag catatttaca
    22141 gtgatgtcga ttatatgctg cttggattta tcgtcgagtc ggtcaccggt cagccgcttg
    22201 accgctatgt tgaagagtcg atttatcgcc cgctcggcct gacacatacg gtatttaacc
    22261 cgctgctgaa aggctttaaa ccgcaacaaa ttgccgccac ggaattgaac ggcaataccc
    22321 gcgatggagt gatccacttc ccgaatatcc gcacctccac tcactggggt caggtgcacg
    22381 atgaaaaagc cttttattcg atgggcggcg tttccgggca cgcaggtttg ttttccaata
    22441 ccggcgatat tgcggtgtta atgcaaacga tgctgaacgg cgggggctat ggcgatgtgc
    22501 agctgttcag tgcggaaacg gtgaagatgt tcaccaccag ctctaaggaa gatgccactt
    22561 ttggcctcgg ctggcgcgtg aatggtaatg ccaccatgac gccgacgttt ggcacgctgg
    22621 caagcccgca aacctacggt cacactggct ggaccggaac ggtgaccgtt atcgatccgg
    22681 tgaatcatat ggcgattgtg atgttaagca acaagccaca ttcgccggtt gccgatccgc
    22741 aaaagaatcc caatatgttc gaaagcggtc agctgccgat tgcaacgtat ggttgggtag
    22801 ttgatcaggt gtatgcggcg ttaaagcaaa agtaataaaa aggccagtca gtaatgttac
    22861 tgcctggcct ttaatcaatg aatcagaaac gcttacagcg ccatcaactt gtccagcgac
    22921 ggtgcgaaat aatagccgcc ggttaccggt ttggtgaaac gcaacatcgc atcacgctta
    22981 ccatcggtat cgccaaacat gctcagcagt tgctgctcaa tgttatgcag acgcgcgcag
    23041 taggcgcaga agtacagacc gtgagtgcca ctggcggtgc cgtacggcag gctctggcga
    23101 acaatcttca gccctttgcc atcttctttc agatcaacgc gggtcaggtg agaggtttcc
    23161 ggacgttcgt cgccgtcaat ctcttcgttg gcctctttgg tgcgcccgat catcatctcc
    23221 tgatcgtgaa cgctcatccg gttgagctgc ttcaggttgt gctcccaacg ctgcacaaac
    23281 acatagctgc cgccggcatc cacgccgtct ttgataaccg ccacttcgcg acgcgtctct
    23341 tcacccgccg ggttttctgt gccgtcaaca aagccgctca ggtcacgctc ttccacccaa
    23401 cggaagccgt ggatctcttc tttcacttca atgcagtcac caaaggcttc catcgccgcc
    23461 tgggcgacag agaagtttac gtcgtgacgc agagaaagaa tgtggatcaa cacatcgaac
    23521 tgggtggtcg gcgcaaggcc tttaccgtag cccggaaaat ctttcagctc ttctgcccca
    23581 acgccgccgc tcagagcgcg ccagatgttg ttaccaaagg caaccaccgc accaagatgc
    23641 gcgtccggga atttcgcttc aaaagttgcc agtttgtcgg caaatgtttt actggccgca
    23701 cgcagggcgt caacttcccc tttcacgttg gcttcgatcc aaatcgccgc gcggcaatgt
    23761 tctggcaaaa tgccactctg aacctgagac attgttcctc ctgaaaataa taatgccacg
    23821 caatccgtgg cattgttgag cgctatttta cgtgtttttt cgcggcggcg gtttgttcag
    23881 acgcaaatta acgccgccag ataattttac tgactttcca gtttttcagg gtatcgtccg
    23941 aaggcattaa cccttcagga ccactccatt ccccgctgaa ctggtagcta atatgttgac
    24001 tgccttcggc tttacattcg acggcacgat tatcatcacc atcagctttt tggcaattgc
    24061 caaatgcttt gctgtaaagg tcgctaaacg gtgtaccgat tttaacaccg gtgtcagcag
    24121 gaatatcgct atccagcacg tcaatgcgac tgatcgtgcc ttgatcgccg ttaatcacca
    24181 tcgccacgtt gtcgcccttc atcacttcaa aaaagcgcac cacgttgccg ttcgcggttt
    24241 tcattccgct gcgcaggcga taatcgccat caaccgcatc ggcaatggct tgttcttgca
    24301 gtggtgtgga cgccgttaat tcaccaacgc cctgctcgct cactttggtg gacgacccaa
    24361 accagttcca cgggttagcg gcagaccagt taactgacga catcgtggaa cagccggtca
    24421 gcatcaatgg catagcgcat aacattaaac gcagcgattt catgtcactt cctttggtta
    24481 ttcaataacg ttgcttggag tgcaaattca ccaaaaagtg ccgttattct tttacttcat
    24541 taaaacaagc gcgtaaacgt cgattggtca gcagccagat taacgccaca atatccgcca
    24601 ctaccagcgc cagaccgata ccgctaacgg attcaccgtt cagccacagc cacggttgcc
    24661 agcaaagtaa aaccacctgc gccaacagca acagaaaata gagtacacgc caggtgcgag
    24721 gaaacgtagc ccgccgaccg ctcaacaaaa acgccagcac cgccggaatg ccaggaatca
    24781 gccccagcca gaaattatcg tgatcgggat aaaacagatt tagcagcgca gtaccctgct
    24841 cgcgcgacgc accggcaatg acaaacagca cccaggttcg cgcctgaagc aatagcacaa
    24901 gccagaagag caagggtaaa cgcaggcgac cgtgcgcatc ataatggaca ggatgaaact
    24961 cagtactctt catcttcaat caaacgctta cccagactca gcacgtcggc gtgttcatat
    25021 cccaggcgtt catacattcc gagcaccatg tcgttatctt ccggcacgtt gatctgaatt
    25081 ttcgggcagc cgcgagcaat cagctttttc tccagccggt taagcaacgc attggcaatc
    25141 ccacgcccac gaaactctgg atgcacgcca agataataag cagacccgcg atgcccgtca
    25201 taaccgccca tcaccgttcc gaccacttca ccgttcacct cagcgaccag aaacaaactg
    25261 acgtcatggt tcatcttacg ctcgatgtcc atttccggat cgttccacgg acgcagcaag
    25321 tcgcaacgct cccaaagggt gatgacctct tcgaaatctt cctggcgaaa tacgcgtatc
    25381 tccatggtat tcgttacctt tttgcgggtt aaaaggctga ttatggcgtg aacggtcgaa
    25441 ttagccaata tctgacgaaa atcggttgaa aaagtggcat aatggggagt tgtcaactat
    25501 tgaaatgaaa agtaaaacaa ttctcaacag caaacggtcg taacggatta cgcgatacga
    25561 tataacatct ggaactttat tattacaact caggccgtat gagcactttt aaaccactaa
    25621 aaacactcac ttcgcgccgc caggtgctga aagccggttt ggctgccctg acgttgtcag
    25681 gaatgtcgca agccatcgcc aaagacgaac ctttaaaaac cagcaacgga cacagcaagc
    25741 cgaaagctaa aaaatctggc ggcaaacgtg tcgttgttct cgatccaggc cacggcggga
    25801 ttgataccgg agcgatcggg cgcaacggtt cgaaagaaaa acatgtggtg ctggcgattg
    25861 ctaaaaacgt ccgttccatt ttgcgtaatc atgggattga tgcgcgttta acgcgttctg
    25921 gcgatacgtt tatcccactt tacgatcgcg ttgaaatcgc ccataaacat ggcgcagatc
    25981 tgtttatgtc aattcatgcc gatggcttta ccaacccgaa agctgccggt gcttcggtat
    26041 ttgccctctc taaccgtggg gcaagtagcg caatggcgaa atacctgtct gaacgcgaaa
    26101 accgcgccga tgaagttgcc ggtaaaaagg cgactgacaa ggatcatcta ttgcagcaag
    26161 tgctgtttga tctggtgcaa accgatacta ttaaaaacag cctgacgctc ggctcgcata
    26221 ttctgaaaaa gattaagccg gtgcataaac tgcacagccg caacaccgaa caagccgcgt
    26281 ttgtggtgtt gaaatcgccg tcggttcctt cggtgctggt ggaaacctcg tttatcacca
    26341 acccggaaga agagcgactg ttaggcacgg cggcgtttcg tcagaaaatc gccacagcga
    26401 ttgctgaagg cgtgatcagt tatttccact ggttcgacaa ccagaaagca cattcgaaaa
    26461 agcgataagt tatgaaaccc gacgcacacc aggttaaaca gtttctgctc aaccttcagg
    26521 atacgatttg tcagcagctg accgccgtcg atggcgcaga atttgtcgaa gatagctggc
    26581 agcgcgaagg tggcggcggc gggcgtagtc gggtgttgcg taatggtggt gttttcgaac
    26641 aggcaggcgt caacttttct cacgtccacg gcgaggcgat gcctgcttcc gccaccgctc
    26701 atcgcccgga acttgccggg cgcagtttcg aggcgatggg cgtttctctg gtagtgcatc
    26761 cgcataatcc gtatgttccc accagccacg cgaatgtgcg cttttttatt gccgaaaaac
    26821 cgggtgcaga gccagtctgg tggtttggcg gcggcttcga tttaacccct ttctatggtt
    26881 ttgaagaaga cgccattcac tggcaccgca ccgcccgtga cctgtgcctg ccatttggta
    26941 aagacgttta tccccgttac aaaaagtggt gcgacgatta cttctacctc aaacatcgca
    27001 acgaacagcg cggtattggc gggctgttct ttgatgatct aaacacgcca gatttcgacc
    27061 actgttttgc ctttatgcag gcggtaggca aaggctacac cgacgcttat ttaccaattg
    27121 tagagcgacg taaagcgatg gcctacggcg agcgcgagcg caattttcag ctctaccgtc
    27181 gcggtcgtta tgtcgagttc aatctggtct gggatcgcgg cactctgttt ggcctgcaaa
    27241 ctggcgggcg caccgagtcg atcctgatgt caatgccgcc actggtacgc tgggagtatg
    27301 attatcagcc aaaagatggc agcccagaag cggcgttaag tgagtttatt aaggtcaggg
    27361 attgggtgta actccctcac ccccattccc gcatccgctg atgcaacgtc aacgacggct
    27421 tctcggcaaa cagctgctgg taatccgtgg caaattgccc cagatgccag aatccccact
    27481 gcatggcggc gtcttttacc gttgtgcttt gcgaccacgg gcttatcagt tcgcggcgta
    27541 cggcgttcag gcgaatgcgt ttcagccacg cgttcgggcc aattcctaaa atagcgtgaa
    27601 acgcgttttg tagcgtgcgg cggctgacat gcagttgatt acacaagtcc agcaccgtca
    27661 ccggttcgga catgttttcc agcacatatt cacgggcgcg ggaaagcaac cgacggtaac
    27721 tctgatgact gatgctttcc gccgtcacca ttggctgcgc ttcttccagc atcgccccca
    27781 tcgccattag caaattatcc cccagcactt ttcgcactgc aggctgatgg agattttccg
    27841 ggttctcgca aaacgtcgcc agcgcctgtt ggacaaagcc ccacagcgcg gctttatgct
    27901 gctctttcac ttccagcgcc gactggctac gcaacatatg taatacccga tccggattat
    27961 gcaaaaagtt agcctgacgg gtgatgacat cttcagaaag cactacgccc aggatcgtgt
    28021 aatcgtccgg cgtacttaat tcaaactcgg ttccaccagg gcgcgtcgct atttctgcgc
    28081 ttcccagaca ttgcgaaccg ataaatccct gctcaccgcg cgtcgccgga atgccaaacc
    28141 agaacgagtt cggccagacc agacacgact gacgcagcgc cagaccggtg tattcacgaa
    28201 aaacctgaat atcatcgagt agaatttccg taaattcacc atgaaacttg cccggatgca
    28261 gctgatcgta aatctgctgc caggcggtaa tcgttaaagc atgttcatag acatccgttg
    28321 tctgtcgttg atgaacatta tccacttcga ccttcggcgt gagcttcagg ttttcgggta
    28381 agggttcatg ataaagatgg tgcaaattgg ctgtacgggt ctttttcatg atgttaatgc
    28441 cgggtgttgt aggacacccg gcacctccga caggttaatg gggcttgaga cgataacgac
    28501 tactgcgttt acgtaacgtc ccagcagaaa agagctgttc gagcgcattt ctggcttttt
    28561 ccagcggcca gccaaagtgg gcagccactt ctcctgccgt cactccctga cgtactgatg
    28621 ttaacagcgc caacaactcg tcagcagatt ccgcgacgat aactggcgcg tcgggttcct
    28681 ttacgggttg cttcggctgg cggttaaagc cagtaaccag ccactgggtg tcatcgtctg
    28741 gccgaccgat ctccttacgg ctgatgacac gcccggtacg catcgcggca gcacaacctg
    28801 cgtccagcgc agcacgacac gccgccagat cgccttccac caccagcgtt aagcgacctg
    28861 ggtcaagcac ttcgtgactg agcagacgaa cgttagctgc tttgagcatg gcatccgctg
    28921 catctatcgc ggcgaccatg ccgtccactt ccagcaatcc cagtgcattg atcattggca
    28981 acctccgtta cgcacgctgg attggattac gcgcgatttc cagcactgca tcggtaaagg
    29041 cgttacaggc cgctttacac gctgcctggc taccggttaa aaatgctgcc gagtagttag
    29101 tttcagacgg tggcgggaca taggtcacca gctgtacgtc ggcagatttc aacgctgcat
    29161 caatgccgta ggtcgcttcc agcggcggtg ccaccagata cgccatcgga tcgccaagcg
    29221 tgatcccggc ggttgatgag agataagaac cggtacgcga aactacatgt gccaggaatg
    29281 ccgtatcttg cgcatcatta gcccactgga aagcagcgcc attttcaata tgcgcaacca
    29341 tcgcatccag accggcacgc acttccgccg ggtttggccc accaagcata atcagcacct
    29401 caccggcagt cggtgacggg ccgtgtgccg cgcccgcata cagcgagcga ccatatacca
    29461 cttctaccat cgcttgtttg gtcgcttcgt cagccgcaat gtaggttacg tcgtcagaat
    29521 cggcagaaat cagaccgagg ctacgaatat gtggcggcaa tttcagttca cgcgcaaaat
    29581 cggcgttaac ggaggcaatc acccgcatgg cggttaccga cggtcgaatc aaatctaaag
    29641 ctggcatgat gcctccttat cgggtcatgt tgatgccgga tgctttctgc tccagcatac
    29701 gtttcgccaa atccacaatg acggctgcgg cttcaaccgg cggcgtgccg ccctggtgaa
    29761 tgttagagat acaggtgcga tcggcctcaa cggtggtcgc catacgcggc gagtaaacgg
    29821 cgtagcagga gaggctttct gactgaccca accccggacg ttcgcccacc agcaggatca
    29881 ctactttcgc gccgaggatc tcaccgatct gatcttcaat cttcacgcga ccataacgaa
    29941 caaagaatgg cgtgccgact ttcagcccgg cctgtttcag gcccgccatc agcggcggca
    30001 ggatctcttc gtagttcaca gtgatggcat cagttgacag gccatcagaa ataacgacct
    30061 gtacgtccgg attagcaaca cactgtgctt tcagcgcttc aacagcttct gcacacaggc
    30121 ggcggcccat atccgggcga gtcaggtaga gatttttgtc gctaatttcg gagcgtactt
    30181 ccagcaagcc ctgcgctttc acccactctt ccggcacttc tttcagtacg gtatctttcg
    30241 aacgagagtg atcggccagg aaacgcagca gcgccagcgt acgcggacgc ggaccggcac
    30301 gaccggtaca aacgcgggcc acggtgctgc gacgcagttc tgttaacaca tccgcacgat
    30361 gtgggttttc aacaccaatc cacacttttg cttccgcaga acctaaatcc agcgcgcagc
    30421 tttccgaggt caccggtgcc gcacaggtgg tagtggcgca ctttgcttct gacggcgcgg
    30481 gggccgtttg tcccattgac gccatcacgc tgcgtacaat ttcttcaatc tgtttttgat
    30541 ccatgatgtg ttatctccgc gtcatcagaa gaacagtgac ggatcgcccg cccgtttggt
    30601 caggcgaccg tttgccataa tgcccatgct ttccagccag cgttcaaact ccggtgacgg
    30661 gcgcaggttg agtaactgac gcacagtggc ggtatcgtgg aatgcggtgg tctggtagtt
    30721 gagcatgatg tcatcaccca gcggcatccc catgatgtag ttgcagcctg cggtggcgag
    30781 caggatcatc aggttttcgt tgaggttctg gtcagcgtca gcgtggttgg tgtaacagca
    30841 gtcacagccc atagagatgc cgctcagctt gcccataaag tgatcttcta agcccgcacg
    30901 gataatctgg cggtcgttgt agagatactc cggcccaata aagccgacca cggtgttgac
    30961 gataaacgga tcgtaatgac gcgccagccc atagttacgt gcttccatcg tcacctggtc
    31021 tgcgccgaag ttagcgccag cggatagcgc tgaaccttgt ccggtttcga agtagaggca
    31081 gttttccccg gcgatacggt tgaactctgc acccactgcg cgcgcttcgt cgagcatcgc
    31141 cagttccacg ccaaactctt tcagcccttt ttcgctgcca cagatactct ggaaaatcag
    31201 tccgccaggc gcgccgcgac gaatcgcttc gatctgggtg gtgacgtgcg ccagtacgca
    31261 gccctgagtt gggatgttga atttgtcgat cacgccatag atggtgtcca gcacgcggct
    31321 taagttttcc acgtcgtcag tcaccgggtt aacgccgatc accgcatcgc ccaccccgaa
    31381 ggaaagccct tcgtagattt gcgcggcgat actttgcacg tcgtcacggg tgtcatttgg
    31441 ctgcaaacgg gcgctgaagg tgcccggaat accgatggtg gtattggcct ttttgattac
    31501 cggcattttc ttcgcgccgt agatcaggtc cgcgttggag caaatcttcg ctaccgccgc
    31561 gaccacttcc gaggtcagcc ctttgcgggt aaaggcaatg tcgtccacgc tggtttcatc
    31621 gctcagcaca tactcacgca gttcgctgat gctccagttt ttaatctggt tgtaggccgt
    31681 ttcgttaaca tcgtcctgaa tcagccgcgt cacgcagtca tcttcatagg caatcaccgg
    31741 attattgcgg atgtccgcta cggtcatttc cgacaacacc tgctttgccg ccacgcgctc
    31801 ctgtgagctt gccgctgcga cgcccgccag cacatccccc gaacgcagtt cgttggcttt
    31861 agccagcacc tcttttacat ccttaaactg atatacattg ccgaacaatg tggtctttag
    31921 tttcataagt cgttccctca ggaaggaaat gcgagtgatt tcaccgtcac cggcacaacc
    31981 gatccgccaa aaagaggcgt accaatgtcg atatagtccc ccgcgcggac aatcacttcg
    32041 tcaatgactg ccaacgggag ttgttgtagc tgtgggcgca acagcatgcc cagagcttta
    32101 ccaaagtcct gcccggccac caccagcagg ggatgcggat tcggaaaacg cgcgacgaaa
    32161 tcgaccaacg cgttgatgac cgtcagtacc gcggcgtaac gcacaggcag cgaggcggga
    32221 agcgccagca cgtacgcgtc agttttggga tcaagatcca gctgaatcag cgcctgttgc
    32281 caggcactca ccagatccgt ttcatcaatc gggatcgcca ccggcaaatt gcgcagcggc
    32341 agttgtacgc cctccagcca gattgtgctg ccagagagcg aaagggtatg tgcgcccgcg
    32401 ccaatcaccg tggcgcgcac ggtttgcgcc ggaaactgca cgttcatctc acgcaggcgc
    32461 ggatggtcat gtagcgccgt cgccagtagc gggccaatat cggcaaaaca gaacgggtca
    32521 gcgggctggt ggcgataaca ttcgcccacg ccgccagaaa gcgtaatgat ttcgggcgta
    32581 acacctgcgg gcagcaaacc ggtttgcatt aatgcctgcg cgagcggcga aagcgttccg
    32641 tcaatcactt cgacaatcag ctctgccatc cgtcgggtaa cctgcaccag ctgcgcgccg
    32701 gtcagcgaac ggacgtcagt gcctgcaccg aaacactcat ccacaatcat ctgccccggt
    32761 ttatgagcgt aaaccacgcg cccctagctg tcggtttcca gcaggcgacc accgacgttg
    32821 aggcaggcag tgccgctgat ttttccggca tcgaacaggg cgtagttcgc ggtgccaccg
    32881 ccgatgtcga tattcagtac ccgacacagc cgttgttcag aaagggtttg cgccccggca
    32941 ccgtgaccgg cgatcacgga ttcgaggtgc ggcccggcgc tggcgacgac aaaatcgccc
    33001 agcgactgag agagcgccat caccgccggg cgagcattgc gggttttcgc gctttcaccg
    33061 gtgatgatga tggcaccaga atcaacgctt tccggcgcaa tgcccgcagc ctgatattgc
    33121 tctaaaatca gcgatttcag ttccgcttct tttaaaccac cctgtttatc gacaggggta
    33181 aagaacaccg ggctttgcca gctaatttcg cgtttgatga attcgtagcg cggcacctgc
    33241 gacaccgccg cacggttaac cagttccagg tgggagaaaa tcacctgggt ggtggtggtg
    33301 ccgatatcga taccgacgct cagtagctgg cgagtgttca cgattgtgcc tccgcttcgg
    33361 ttttagccgc ggtcgcgtcg tcttttggca ccagcatcat cgccacgcca atcgccgtta
    33421 cgccgccgat caacttgccg acaatcatcg ggaagatcat ggcattcatg ttggcagcgg
    33481 cgaagcctaa gtggtcgccc agggcgaaag cagcggaaac ggcgaaggcg cagttgatga
    33541 ctttgccgcg ggtatccatc tgcttcatca tgccgaacat cgggatgttg ttggcaagcg
    33601 ttgccaccat gtcggctgcc gcgatgttgt tcatattcag cactttaccg acgctcatca
    33661 gcggtttttc aaaccagcgg gtcagcagca gcaccatcgg atacgcccct aacagaacgc
    33721 aggagataga accgataact tcaatggcgc gcatcacctc accgggttta tcgccagggg
    33781 ccataaagat aggatccaga ccggggatca gttcccagcc aagcaggaat ttcactaccg
    33841 cagcggcaag accgagggtg atcaatgcaa cgaggaattt ggcgaagatc tggaagccgt
    33901 tgatcatttt ttctgggatg aatttcagcc ccagcgccac cagaatcgca acaatgatca
    33961 ccgggatcat gttcatcagg atcagggcga aagtgaattc caccggctgg ccgttgatct
    34021 gaacaccgga gtacatagca accagaccac cagcgataca accaatcgga atggtcacaa
    34081 tgcccgccag cacgccgagc gccagataac gacggtcaga aggttcgata atgccgagcg
    34141 ccaccggaat ggaaaacaca atcgttggcc ccatcatcga cccgagaatt aacccagagt
    34201 atagccacgc ggctacgtcg ccgcccgcca gctctttggc gaggaagaag ccgcccatat
    34261 cgcacgccag cagtgttccg gcgaacatcg atgggttagc gccgagcatt tcgtaaaccg
    34321 gaataattac cggcccgagt acgtgagcca gtaccggtgc cagcgcggtc ataccgacca
    34381 tcgccaggcc cagtgcgccc attgccataa agccttcttc gaactgaccg ccggatcctt
    34441 cgatactttt accgaactta ccgaggaaac gagcagaacc gccgaactgt gacaggatcc
    34501 tgtcaacggc agctatcagc ataaagaaca tcatgatgta catgatgatt tcgttaattc
    34561 ccatcgcctt tactccctgt tagttgttat ttattagcgg atgctgcgtg aagccttatc
    34621 cgcactacat gtgcaatccc acagaccgga taagacgcgg catttattgc gccgctgcgt
    34681 acaggccgac aagctgttcc aggctggcgg tacgcgggtt gctgcgcaga caaatatctt
    34741 ccagcgcggc ctgcgcccat gcaccgtaat gcgcagatgt cgcgccaaca tcgcccagtc
    34801 gtttaccaat cccaacttcc gcaatcagct cacttaccgc gttaatagcg tcacgatcgt
    34861 cggatttttt agttcgcagt gcccgaccaa tctgactaaa gcgttcgcga caaaccatcc
    34921 ggttaaactc catcaccgtt ggcagcaaca tagcgttcgc gagaccgtgc ggaatatgca
    34981 gcgccgcgcc cggctgatgc gccatcgcgt ggcacaaccc aagacccgca ctggaaaacg
    35041 ccattcccgc catacatgaa gccagcaaca tgctctcgcg cgcggcaagg tcgtgaccgt
    35101 agcccaccgc tttcggcagc gatttgccaa tcatcgcaat ggcaccaatc gccagactgt
    35161 cggtaaacgg tgtagcgttc agggcgctgt atgcttcaat ggcatgggtt aacgcatcaa
    35221 tgccggtcat cgccgtgaca tgcgacggca caccttcggt caatgcggcg tcgaggatcg
    35281 ccacatccgg catcagcgag gcatgggcta acacctgctt gcgcccgctc accgcgtcga
    35341 taatcaccgt tacattggtg gtttcagagc cggttccggc ggtagttgga atggcaatca
    35401 gcggcaagcg cggttgcaga acgctggttt ctgacatctc tgccagcgtg ctatccgggt
    35461 tcgtcaccag caacgccacg gctttcgccg catccagcac cgagccgccg ccaaatgcga
    35521 tcaccccgtc acaacctgac tcacgcaact gcgccacggc tgcacacacg tcggtaatgc
    35581 acggttcgcc caccggacat ggccagagcg tcatggcgat acctttaacg gccaggctac
    35641 gcgtcagccc ggcggtcatc cctgcctgat gcaaaaagct gtctgccatc acgaacagat
    35701 gtttcagccc acgcgtttgc gcttgctgtc cgcaactgct caccgagccc ggaccgcaaa
    35761 gcgtcaccgg tggaacgcta aatgttttta cccgttgcag attcagggta tcgaacgcct
    35821 gaaagagcgc ggtctgcaat tcattttgca tatagcccct ccgctttctc tcgcccacta
    35881 ctggcgatcg ccagcggggt cataaacaaa ctgtgctgcg gtaaatgcac ctgtaacgcc
    35941 gggaattgtt tgcgaaacag ttctgccacg cccggttgca tacaggagcc gcccgccagc
    36001 cataaatcag taatgccctg cccttcaata tgccgggcga caatgtccgc cattttttcg
    36061 tacaccggtt tcaccgtagg ccaaatctct tcaccgtgac cgcgcttgta ctgctccgcc
    36121 tcttccagcg aaatacggcg atttccggcg aaagtcagag agatgtgatg tccgccggtg
    36181 gcttcatccg ccgagtacgt cactttgccc tttttcacga tggcaatgcc ggtagtgcca
    36241 ccgccgatat ccactacacc ggcgttgtcc agttgcagca gatccgcgac tgcggttggc
    36301 tcatccagca catggctcac ttccagcccg gcagactcca gcacgttgat ggaaatacgc
    36361 gggtccgtac ctggaggaaa tgaagtcgcc gcatggctaa aacgacgacc gaattgctgc
    36421 tcgagcgtgt cgagatggcg acgaacaatg gtgaccgcgc cgaagaaatc ccagacgatg
    36481 ccgtcgcgaa ccacatcggc ccagtcgagg cataccgcca ctggctgacc gtcgcggtcg
    36541 acaaccatcg acaccacatc gcaggtgccc agatccactc ccagccacag cggagattcc
    36601 gtcgcggcgg gcgtctggtt acacagcgtt gccgccgttt gcagacgtgg ggtgagccat
    36661 tgttcgtcgt gcgccatctg ttactcctta aacaatgcga aacgcatcga ctaatacaca
    36721 gcgacgcaga cggacaaacg tacgcgcgct ggtcacccct tcaccggttg gcgtggtgat
    36781 ggtcatggtg gtccagcctt ccccgcccag ccccagcccg gcaatgcacg gtccgttctt
    36841 aacgaaaatg ctggtatcaa tagcattcgc catctggttc atgttttcga tgttgcgcga
    36901 gtgcattgcc gccgtgtggt ggcaaccgcc ttccagtttc accgctagcg caatggcatc
    36961 cgccacgttg gcgacgcgca cgacgggcaa caccggcatc atcagttcag tcacggcaaa
    37021 cggatgttct gcggtggttt ccacaaacag caggcgcgtt tcttgcggaa ctttaaggcc
    37081 gattgccgcc gcgattttgc ctgcgtcgcg accaacccag tcacggctga cggtgccttt
    37141 tccgcgctcg tcgatatttt tcagcaacac cggttgcagc tgctgcgcct gttctgcggt
    37201 cagtttcacc gcgtgctggc cttccatcag acgcatcagt tcatcggcta cgctatcaac
    37261 aacaatcagt accttttcgt cggcacaaat gatgttgtta tcgaaagacg cgcctttgac
    37321 gatggactga gcagcacggg cgaggtcagc ggtttcatcc accactaccg gcgggttgcc
    37381 agcgcctgcg gcaatcagac gtttattggt gtgtttacgc gccgcttcta ctaccgcttc
    37441 gccgccggtc accaccagca ggccgatacc cggaaacttg aacaagcgtt gcgcggtttc
    37501 gatatccgga tttgccacag taaccagtaa gttttccggc ccgcctgcgg caacaatcgc
    37561 ctggttaagc agcgtgatcg cccgctggga gacttttttc gccgccggat gcggggcaaa
    37621 aatgacgctg ttgcccgcgg caatcaggct gatggcgtta ttaattacgg ttgccgccgg
    37681 gttagtggac ggcgtcaccg aagccaccac gccccagggc gcgttttcaa ttaaagtcag
    37741 gccgttgtcg cccgtcagca cttgcggaga gagacactca acgcctggcg tgccgcgtgc
    37801 ctgagcgacg tttttggcaa atttatcttc aacgcgaccc atgccggttt cactgacggc
    37861 aagttccgct aaatctctgg cgtgtttttc gcctgcttca cgaatggcag caatggctaa
    37921 ctggcgcatt gccacgctct ttaacccttg ctgggcaatt ttggctgccg caacagcgtc
    37981 atccagggac gcgaaaacgc ccatctcatg aacggcggcg ggcggcgtgt cactgctttg
    38041 cattttcagc agtaccgctt tcaccacctg ttcaatatcc tgttgattca tgatgttctg
    38101 ccttatttgt ggaaaattac ctgaccgcca gacaccacct catcgacgat gccaatcacg
    38161 cacagatcga ccggtgacgt ttcgctttta tgcgcctggc gggcggaact gccactcccc
    38221 ggttcccgcg ccaatattgt cgatggcgac ggcacattgc ccgtcaggat taccatgtgg
    38281 atcaatcatt tccaccatca gcaatttgtc atgcgccagt ccgtgatggc gtacggtaca
    38341 aacaatttgt ccagtgacga ctgccagttt catacccgcc tccgtggcgt atttcaggta
    38401 aaagctcccc ctaccctccg cggaaggtaa aatgaaaaat gagagagcgt gacgcccgaa
    38461 tcgacgtcac acagggtgat tacaggttgc tgctatcgcc tttcaggccg atcgggaaca
    38521 cttcttccag gtcgccgtgc ggacgcggga taacgtgtac agataccagc tcgccgatac
    38581 gctgtgccgc tgcggcaccg gcatcggtcg ccgctttaca ggctgcaaca tcaccacgaa
    38641 ccatggcagt acacaggccg ccgccaatct gcttaacacc aaccagcttg acgcgcgcag
    38701 ctttcaccat cgcgtcagaa gcctcaatca gtgcaaccag gccccgggtt tcgatcattc
    38761 ctaatgcttc cattgtgctt tcctctttat cagggtccag aacgggaccg ttcattcaac
    38821 cagtgtttgt aaactgcttt cgcggttcac ttctgtctga cgcggcacag ctgccaccag
    38881 cgccagctcg ataatttcct gcacactaca accacgagag agatcgtgca tcggcgcggc
    38941 aagtccttgt atcaatggcc cgacggcacg atatccgccg agtcgttgtg cgattttgta
    39001 gccaatattt ccggcttcca gcgacggaaa aaccatcaca ttggccttgc cctgtagcgg
    39061 gctggcaggc gctttttgcg ccgccacttc cggcacgaag gcggcgtcaa actgcaactc
    39121 gccatcgact accagctttg gtgcgcgctc acggacgatt tctgtcgcct gctggacatt
    39181 ggcaacgcag gggtgacggg cgctaccgtt actggaaaac gacagcatcg ccacgcgcgg
    39241 ctcttctccg gtgatggcgc gccaggtttc ggcgctggca agcgcgatat ccgccagctg
    39301 cgccgccgtc ggctgtggta ccacgctgca atcggcaaag cccaacgccg gaccgctgta
    39361 ctgtggcagc atcaggaaaa tggatgagag cgttttacag cctggctgca agccgataat
    39421 gcgtaatccg gcacgcagca cattcgccgt ggaagagagg ttgcccgcga tacagacatc
    39481 cgctttaccg gcgctgacca ttgcggcggc gaacatcagc ggatcggtaa gtttttccag
    39541 cgcatccggc ggcgtttttt cgcccgcgcg ggccagccag cgatgagcaa attcttcccg
    39601 cattgcgagg ttgccatgcg gatctatcac ctgtagcccg tccatcgcca cgccgtgact
    39661 gagcgcaaac tgacgaagtt caaacggatt ggcgaccaga atgggcgttg ccagaccttg
    39721 ttgatgtaaa tattgcgcag ctttcagcac acgttgatct aacgcatccg gaaaaaccac
    39781 tctggcgggc gctcgcaacg ccagttcaca acaacgttca ataatcattg cgtttccccc
    39841 agtcgttgtt ggatctgctt caccgttagc ggctgcttcg ttacgctcag gatcatcatc
    39901 acgtagaccg tgctggaaag gcggttcagc gcctgcaaga tgtccgggcg taatacttca
    39961 aaactgcggg tgataaacac ctgcgccgct acggtttcgg cttcccgcac tttggtgcgt
    40021 agcagattca gcaacgccgc atcgcgcccg tggcttgctt ccggcaccag atgatcgtga
    40081 ccaagataac gcaacggctg atgagaaagc cggtgcagat cttcgtcaga aagcccgaca
    40141 atcgcctggc agcccagcgg ttccgccagt tcaatttgca gccacactgc cagcgcaatg
    40201 gtgctgtcga gaactgcgcg aaaacccaga cgcggatcgc ttttggcgac cattttttcc
    40261 gccgacaggt gagtcagcgt atccggcttt tttgccaccg gctggcggca cagttcgcag
    40321 cacgcctgcg gatgttcatc gctactggtt agcccatgaa cgggctgcgg ctgctgctgt
    40381 tcatcgtcaa caaacaggcg gccctgctcg tcaataaact tgatgcgcag atggcggctt
    40441 tccagcaact cccgggcaga gggcgtcagg cgactgtccg cagggagatg gatctctgcg
    40501 ccttcgctga gcgtatggtt cgctcttagc catgcttcgg tgatgaaatc tttcataggg
    40561 attgccagtt agccggccag gcgacataca ggaatttcac gctggatgtc gtaccaaatt
    40621 cgatgctgga gcctttcggg ataaacatca cgtcgcctgc tttagcgatc atggtttcgc
    40681 cttcgtggcg aacatggagc tccccttcca gcaccatgtc gatttcgtcg tagttcaggg
    40741 tccacgggaa gaatgcgttc tcccactgca taaaaccggc ggccatgctg ctgccgtcgt
    40801 ctccggtcac aagatcagtc aagcccacgc aatgcggttg tgcgccatca aagcgaccaa
    40861 acttgacgct gctaccatca atcactttga tgccgccttt gccggtcacc gatttgaagc
    40921 tcggctgcat tgcaccctgc tccagcgact gtttttcctt catcactttt tccatcagct
    40981 gcgcgaccag gctttcggta aactggcctt ccggtagctg ggcgataatg gtttcgcgga
    41041 tgcgctggct ttcggtttta tcggctggca cgctggcggg cacggacgcc gttaccggga
    41101 tcaattcatc acattcggtg atggtaaaac ccagtagatc cgccacttca cgggcttccg
    41161 gggtaataat gctggcgcgc agaaccacgg acattgcctg ttcgccgcgc gcgtgtgctt
    41221 cacgaatatc attcgctgtg atgagttttt tcacctgctt cctcctgttt ggtgagagat
    41281 gccagataat ccaccagttg ctgcacgctt tgcgggtcgt ggctgtttag ctcgaaaatc
    41341 ggctcctcga agcccgtctc aagcaataac tttcgcgttg cggcgacgtc agcatccggc
    41401 atgtccgttt tactgatgac ggcgatttgc cgtttgctga cgccaatatc cagcaacccg
    41461 gcaggcaacc gactttccgg gtcattcgcg ccgtggacat agatcagcat gtccacatcc
    41521 tgtagcgtgg taattaaggc gtgataccag cggggatggc taaaatattc acccggcgta
    41581 tcaatatcgc ccttatcatt aaattccacg gcctgtgttt ttctggcgag ggtataattc
    41641 ccctgcaacg cattaaataa cgttgttttc cctgccccga cggtacccac aaacgcaatt
    41701 cgtttcatga atgcctcgta ttaacttttg gtcatttcac aaagcgtata atttaataag
    41761 cgacctaaac cactgacggt ttgtgataac gcttcttcta ctgcgcccac cgagccataa
    41821 atcaccaatg caccactgaa gcggtcgaga aatccgatat gcacatcggc ggctttcagc
    41881 gctaaatcac cggcgatcat cgccgtttcg ccgggagtta gcgtcataat gccaattgcg
    41941 cccgcatccg gaacgccgat tttcttcgcc agttcctcgc cgggatgcgc gatgagatgc
    42001 gccagtgtga cctgtttgcc gggcacaaat tcctgaatga tgcgttcttt atccatgagt
    42061 cgccgcctgt gaaaaacttt cgtgtatcgt gacaattttt cgtcagagta aataacaaaa
    42121 ttcggcaagc gcgtttaaaa ggtgaggtag atcactaaga atggagagat atttactgtt
    42181 atgaataaac tttcatatgc gacgcatgaa tataaa
//
LOCUS       DAZFBR010000026        41495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFBR010000026 DAZFBR010000000
VERSION     DAZFBR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.026"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(263..910)
                     /gene="ychE"
                     /locus_tag="ACHJZ2_001631"
     CDS             complement(263..910)
                     /gene="ychE"
                     /locus_tag="ACHJZ2_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO4567769.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(941..994)
                     /gene="ychE"
                     /locus_tag="ACHJZ2_001632"
     CDS             complement(941..994)
                     /gene="ychE"
                     /locus_tag="ACHJZ2_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO4567770.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1387..4062
                     /gene="adhE"
                     /locus_tag="ACHJZ2_001633"
                     /gene_synonym="adhC"
     CDS             1387..4062
                     /gene="adhE"
                     /locus_tag="ACHJZ2_001633"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO4567771.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4363..4980)
                     /gene="tdk"
                     /locus_tag="ACHJZ2_001634"
     CDS             complement(4363..4980)
                     /gene="tdk"
                     /locus_tag="ACHJZ2_001634"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO4567772.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5584..5997
                     /gene="hns"
                     /locus_tag="ACHJZ2_001635"
     CDS             5584..5997
                     /gene="hns"
                     /locus_tag="ACHJZ2_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO4567773.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6141..7049)
                     /gene="galU"
                     /locus_tag="ACHJZ2_001636"
     CDS             complement(6141..7049)
                     /gene="galU"
                     /locus_tag="ACHJZ2_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO4567774.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7251..8264)
                     /gene="rssB"
                     /locus_tag="ACHJZ2_001637"
     CDS             complement(7251..8264)
                     /gene="rssB"
                     /locus_tag="ACHJZ2_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO4567775.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8356..9261)
                     /gene="rssA"
                     /locus_tag="ACHJZ2_001638"
     CDS             complement(8356..9261)
                     /gene="rssA"
                     /locus_tag="ACHJZ2_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO4567776.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9374..9832
                     /gene="ychJ"
                     /locus_tag="ACHJZ2_001639"
     CDS             9374..9832
                     /gene="ychJ"
                     /locus_tag="ACHJZ2_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO4567777.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9882..10724
                     /gene="purU"
                     /locus_tag="ACHJZ2_001640"
     CDS             9882..10724
                     /gene="purU"
                     /locus_tag="ACHJZ2_001640"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO4567778.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10884..10968
                     /locus_tag="ACHJZ2_001641"
     tRNA            10884..10968
                     /locus_tag="ACHJZ2_001641"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10918..10920,aa:Tyr,seq:gta)
     gene            11013..11143
                     /locus_tag="ACHJZ2_001642"
     ncRNA           11013..11143
                     /ncRNA_class="other"
                     /locus_tag="ACHJZ2_001642"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11178..11262
                     /locus_tag="ACHJZ2_001643"
     tRNA            11178..11262
                     /locus_tag="ACHJZ2_001643"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11212..11214,aa:Tyr,seq:gta)
     gene            11309..11440
                     /locus_tag="ACHJZ2_001644"
     ncRNA           11309..11440
                     /ncRNA_class="other"
                     /locus_tag="ACHJZ2_001644"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11487..11618
                     /locus_tag="ACHJZ2_001645"
     ncRNA           11487..11618
                     /ncRNA_class="other"
                     /locus_tag="ACHJZ2_001645"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11802..12479)
                     /gene="narI"
                     /locus_tag="ACHJZ2_001646"
     CDS             complement(11802..12479)
                     /gene="narI"
                     /locus_tag="ACHJZ2_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO4567779.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12479..13189)
                     /gene="narJ"
                     /locus_tag="ACHJZ2_001647"
     CDS             complement(12479..13189)
                     /gene="narJ"
                     /locus_tag="ACHJZ2_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO4567780.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13186..14724)
                     /gene="narH"
                     /locus_tag="ACHJZ2_001648"
     CDS             complement(13186..14724)
                     /gene="narH"
                     /locus_tag="ACHJZ2_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO4567781.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14721..18464)
                     /gene="narG"
                     /locus_tag="ACHJZ2_001649"
     CDS             complement(14721..18464)
                     /gene="narG"
                     /locus_tag="ACHJZ2_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO4567782.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(18857..20248)
                     /gene="narK"
                     /locus_tag="ACHJZ2_001650"
     CDS             complement(18857..20248)
                     /gene="narK"
                     /locus_tag="ACHJZ2_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO4567783.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            20587..22383
                     /gene="narX"
                     /locus_tag="ACHJZ2_001651"
     CDS             20587..22383
                     /gene="narX"
                     /locus_tag="ACHJZ2_001651"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO4567784.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            22376..23026
                     /gene="narL"
                     /locus_tag="ACHJZ2_001652"
     CDS             22376..23026
                     /gene="narL"
                     /locus_tag="ACHJZ2_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO4567785.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(23027..24421)
                     /gene="ychO"
                     /locus_tag="ACHJZ2_001653"
                     /gene_synonym="iatA"
     CDS             complement(23027..24421)
                     /gene="ychO"
                     /locus_tag="ACHJZ2_001653"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO4567786.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            24607..24960
                     /gene="ychN"
                     /locus_tag="ACHJZ2_001654"
     CDS             24607..24960
                     /gene="ychN"
                     /locus_tag="ACHJZ2_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO4567787.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(25004..25699)
                     /gene="chaC"
                     /locus_tag="ACHJZ2_001655"
     CDS             complement(25004..25699)
                     /gene="chaC"
                     /locus_tag="ACHJZ2_001655"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO4567788.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(25860..26090)
                     /gene="chaB"
                     /locus_tag="ACHJZ2_001656"
     CDS             complement(25860..26090)
                     /gene="chaB"
                     /locus_tag="ACHJZ2_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO4567789.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            26360..27460
                     /gene="chaA"
                     /locus_tag="ACHJZ2_001657"
     CDS             26360..27460
                     /gene="chaA"
                     /locus_tag="ACHJZ2_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO4567790.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            27859..27966
                     /locus_tag="ACHJZ2_001658"
     CDS             27859..27966
                     /locus_tag="ACHJZ2_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4567791.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            28395..28502
                     /locus_tag="ACHJZ2_001659"
     CDS             28395..28502
                     /locus_tag="ACHJZ2_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4567792.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(28650..29504)
                     /gene="kdsA"
                     /locus_tag="ACHJZ2_001660"
     CDS             complement(28650..29504)
                     /gene="kdsA"
                     /locus_tag="ACHJZ2_001660"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO4567793.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(29540..30349)
                     /gene="sirB1"
                     /locus_tag="ACHJZ2_001661"
     CDS             complement(29540..30349)
                     /gene="sirB1"
                     /locus_tag="ACHJZ2_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO4567794.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(30353..30745)
                     /gene="sirB2"
                     /locus_tag="ACHJZ2_001662"
     CDS             complement(30353..30745)
                     /gene="sirB2"
                     /locus_tag="ACHJZ2_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO4567795.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLTLVVLYIIIKLATTKVPLLG"
     gene            complement(30742..31575)
                     /gene="prmC"
                     /locus_tag="ACHJZ2_001663"
     CDS             complement(30742..31575)
                     /gene="prmC"
                     /locus_tag="ACHJZ2_001663"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4567796.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(31575..32657)
                     /gene="prfA"
                     /locus_tag="ACHJZ2_001664"
     CDS             complement(31575..32657)
                     /gene="prfA"
                     /locus_tag="ACHJZ2_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO4567797.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(32699..33955)
                     /gene="hemA"
                     /locus_tag="ACHJZ2_001665"
     CDS             complement(32699..33955)
                     /gene="hemA"
                     /locus_tag="ACHJZ2_001665"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO4567798.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34169..34792
                     /gene="lolB"
                     /locus_tag="ACHJZ2_001666"
     CDS             34169..34792
                     /gene="lolB"
                     /locus_tag="ACHJZ2_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO4567799.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            34792..35643
                     /gene="ispE"
                     /locus_tag="ACHJZ2_001667"
     CDS             34792..35643
                     /gene="ispE"
                     /locus_tag="ACHJZ2_001667"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO4567800.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            35794..36741
                     /gene="prs"
                     /locus_tag="ACHJZ2_001668"
     CDS             35794..36741
                     /gene="prs"
                     /locus_tag="ACHJZ2_001668"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO4567801.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            36866..38545
                     /gene="dauA"
                     /locus_tag="ACHJZ2_001669"
     CDS             36866..38545
                     /gene="dauA"
                     /locus_tag="ACHJZ2_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO4567802.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(38600..38878)
                     /gene="ychH"
                     /locus_tag="ACHJZ2_001670"
     CDS             complement(38600..38878)
                     /gene="ychH"
                     /locus_tag="ACHJZ2_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO4567803.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            39156..39740
                     /gene="pth"
                     /locus_tag="ACHJZ2_001671"
     CDS             39156..39740
                     /gene="pth"
                     /locus_tag="ACHJZ2_001671"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO4567804.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            39857..40948
                     /gene="ychF"
                     /locus_tag="ACHJZ2_001672"
     CDS             39857..40948
                     /gene="ychF"
                     /locus_tag="ACHJZ2_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO4567805.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT        10376 a        10767 c        10136 g        10216 t
ORIGIN      
        1 gattattatt tagcactttc ctctaccaaa ggcagtggaa ttactaatat catttcaatt
       61 attcttcagc aagcccagga ttatgatgtg gcgaaaataa catgatgatg aggagaagca
      121 gttaaggaaa tccttataac taactgtaat ttgagaagaa tatttttacc tattcctttt
      181 gcttttgata actgcgcggg aacaaagtaa aaataatgac gaagtaaacg gagcatggca
      241 gctccgtttc atttgaaagg aattaattaa gcaggccggg gaaaatcccc ttaataccag
      301 taacgataaa ttcaatcccc aatgccatca gcaatagccc cataatacgc gtaatcacgt
      361 tgatgccggt ctggcgtaaa acccgtacca gccacggtgc catgcggaac aatccccaac
      421 aacataaagc gaacaatgca atagccacaa agaaaccaaa cagatagcta atgctgtgat
      481 aacgcgtacc ccagacgatg gtagaactga tcgcccctgg ccccgccatc aacggcaacg
      541 ccaatggcac cacaccaata ctttcacgta ccgcggtttc tgatttttct tgcttgttct
      601 gtttatcctc gccaagcttg ccgctgatca tcgacatcgc tatcgtcacc accaggatgc
      661 ccccggcgat acggaacgaa tcaattgata taccaaaaag ttgcagaatc gtgtcgccga
      721 gaaaaagcga gatccacaag ataatggcca cagacaggtt ggctgtaagg ttagttttgt
      781 ttcgcgctgc cgctgtctga taactggtca tgctgataaa gacgggaata atccctaccg
      841 ggttgaccag cgcaaataac ccgatgaaaa atttgaagta aacgggaaaa tcaaaaaggg
      901 tctgaatcac ggttagctcc gaagcaaaag ccggataatg ttagccataa ataaggttga
      961 aaagacgcgc tgacaatacg ccttttgaca gcatttttca cctcctaact acttaaaatt
     1021 gctatcattc gttattgtta tctagttgtg caaaacatgc taatgtagcc accaaatcat
     1081 actacaattt attaactgtt atctataatg gcgaaaagcg atgctgaaag gtgtcagctt
     1141 tgcaaaaatt tgatttggat cacgtaatca gtacccagaa gtgagtaatc ttgcttacgc
     1201 cacttggaag tgacgcatta gagataataa ctctaatgtt taaactcttt tagtaaatca
     1261 cagtgagtgt gtgcgcgagt aagcttttga ttttcatcgg ttaagcaaat catcaccgca
     1321 ctgactatac tctcgtattc gagcagatga tttactaaaa aagtttaaca ttatcaggag
     1381 agcattatgg ctgttactaa tgtcgctgaa cttaacgcac tcgtagagcg tgtaaaaaaa
     1441 gcccagcgtg aatatgccag tttcactcaa gagcaagtag acaaaatctt ccgcgccgcc
     1501 gctctggctg ctgcagatgc tcgaatccca ctcgcgaaaa tggccgttgc cgaatccggc
     1561 atgggtatcg tcgaagataa agtgatcaaa aaccactttg cttctgaata tatctacaac
     1621 gcctataaag atgaaaaaac ctgtggtgtt ctgtctgaag acgacacttt tggtaccatc
     1681 actatcgctg aaccaatcgg tattatttgc ggtatcgttc cgaccactaa cccgacttca
     1741 acagctatct tcaaatcgct gatcagtctg aagacccgta acgccattat cttctccccg
     1801 cacccgcgtg caaaagatgc gaccaacaaa gcggctgata tcgttctgca ggctgctatc
     1861 gctgccggtg ctccgaaaga tctgatcggc tggatcgatc aaccttctgt tgaactgtct
     1921 aacgcactga tgcaccaccc agacatcaac ctgatcctcg cgactggtgg tccgggcatg
     1981 gttaaagccg catacagctc cggtaaacca gctatcggcg taggcgcggg caacactccg
     2041 gttgttatcg atgaaactgc tgatatcaaa cgtgcagttg catctgtact gatgtccaaa
     2101 accttcgaca acggcgtaat ctgtgcttct gaacagtctg ttgttgtcgt tgactctgtt
     2161 tatgacgcag tacgtgaacg tttcgcaacc cacggcggct atctgttgca gggtaaagag
     2221 ctgaaagctg ttcaggacgt tatcctgaaa aacggtgcgc tgaacgcggc tatcgttggt
     2281 cagccagcct ataaaattgc tgaactggca ggcttctctg taccagaaaa caccaagatt
     2341 ctgatcggtg aagtgaccgt tgttgatgaa agcgaaccgt tcgcacatga aaaactgtcc
     2401 ccgactctgg caatgtaccg cgctaaagat ttcgaagacg cggtagaaaa agcagagaaa
     2461 ctggttgcta tgggtggtat cggtcatacc tcttgcctgt acactgacca ggataaccaa
     2521 ccggctcgcg tttcttactt cggtcagaaa atgaaaacgg ctcgtatcct gattaacacc
     2581 ccggcttctc agggtggtat cggtgacctg tataacttca aactcgcacc ttccctgact
     2641 ctgggttgtg gttcctgggg tggtaactcc atctctgaaa acgttggtcc gaaacacctg
     2701 atcaacaaga aaaccgttgc taagcgagct gaaaacatgt tgtggcacaa acttccgaaa
     2761 tctatctact tccgccgtgg ctccctgcca atcgcgctgg atgaagtgat tactgatggc
     2821 cacaaacgtg cgctcatcgt gactgaccgc ttcctgttca acaatggtta tgctgatcag
     2881 atcacttccg tactgaaagc agcaggcgtt gaaactgaag tcttcttcga agtagaagcg
     2941 gacccgaccc tgagcatcgt tcgtaaaggt gcagaactgg caaactcctt caaaccagac
     3001 gtgattatcg cgctgggtgg tggttccccg atggacgccg cgaagatcat gtgggttatg
     3061 tacgaacatc cggaaactca cttcgaagag ctggcgctgc gctttatgga tatccgtaaa
     3121 cgtatctaca agttcccgaa aatgggcgtg aaagcgaaaa tgattgctgt caccaccact
     3181 tctggtacag gttctgaagt cactccgttt gcggttgtaa ctgacgacgc tactggtcag
     3241 aaatacccgc tggcggacta tgcgctgacc ccggatatgg cgattgtcga cgccaacctg
     3301 gttatggaca tgccgaagtc cctgtgtgct ttcggtggtc tggacgcagt aactcacgcc
     3361 atggaagctt atgtttctgt actggcatct gagttctctg atggtcaggc tctgcaggca
     3421 ctgaaactgc tgaaagaata tctgccagcg tcctaccacg aagggtctaa aaatccggta
     3481 gcgcgtgaac gtgttcacag tgcagcgact atcgcgggta tcgcgtttgc gaacgccttc
     3541 ctgggtgtat gtcactcaat ggcgcacaaa ctgggttccc agttccatat tccgcacggt
     3601 ctggcaaacg ccctgctgat ttgtaacgtt attcgctaca atgcgaacga caacccgacc
     3661 aagcagactg cattcagcca gtatgaccgt ccgcaggctc gccgtcgtta tgctgaaatt
     3721 gccgaccacc tgggtctgag cgcaccgggc gaccgtactg ctgctaagat cgagaaactg
     3781 ctggcatggc tggaaacgct gaaggctgaa ctgggtattc cgaaatctat ccgtgaagct
     3841 ggcgttcagg aagcagactt cctggcgaac gtggataaac tgtctgaaga tgcattcgat
     3901 gaccagtgca ctggcgctaa cccgcgttac ccgctgatct ccgagctgaa acagattctg
     3961 ctggatacct actacggtcg tgattatgta gaaggtgaaa ctgcagcgaa aaaagaagcc
     4021 gctccggcta aagctgagaa aaaagcgaaa aaatccgctt aatcagtagc gctgtctggc
     4081 aacataaacg gccccttctg gggccgtttt tttgtttacc caaagcaact tttccataaa
     4141 ccgacagcat tagccttcat catatttgcg acgatgtata acgcctaaac acagggatat
     4201 tgtactttac aggtcacaag tcaacgtcgg tgcttaagag ccctgtgagg cgtatagcgg
     4261 cgttaaaaac tgccgagaag ggtgtatagc ccggaagaag tgcgtaaaac gaactgacag
     4321 gataaaagtg ccctgctcac cctgtcagta aagaaattct tattaatcgt ggcgatgcct
     4381 ttcctgaata gccgttaatg agccgacttg taacgcctct ttatagtgtt tacggcatac
     4441 agaaacgtat cgttcattac caccaataac cacctgctca ccttcgttat aaggtctgcc
     4501 tgcttgatca agacgcagca ccatgcttgc tttacggcca caaaaacaga tggtttttaa
     4561 ttcaaccagt ttgtcggacc atgccagtaa gtattggctg ccaataaata attcacctcg
     4621 aaaatcggta cgtaaaccat aacaaagtac gggtatatcg agttgatcga caacctccga
     4681 taattcatat acttgttgtc tggttaaaaa ctggcattca tcaaccagta cgcaatgaat
     4741 tgcctgctgc tcatgttccg cacgaatctc atcaaataat gatgaatttt ggttaaataa
     4801 ttttgcaggc gatgacaaac ctatacgcga actgactttc ccggcaccaa agcgatcatc
     4861 aatttctgcc gtatatacga cagtgcgcat gccgcgttcc tggtaattgt atgaagattg
     4921 caacaatgct gtagacttac ccgcattcat tgcggaatag tagaaatata gctgtgccat
     4981 cagccacagg ccctcaatga tatgaaataa agttggctgg agtttatcat aattcgtcag
     5041 tttttcagct caacagtatg cacagaagtg gtatatgcgt tctcccttac gaagccttgc
     5101 ataatccttc tgagctatca ttacaactgc ctcgcttgtt ataagcgggt aaatgactgc
     5161 tggtaactat tcacaatctt taacctgttg cgcaagtaat agccctctgt tgacctccag
     5221 gagatagtgc aatactaagt ccctgctctt attgcgactg ttctactttt catcattcgc
     5281 ttaataggga attctcgtaa acacgactaa tacagaagac tgaaaggtcg tcagcctacg
     5341 ataatctccc cataaaatgt gacatgaatc aggaagtttt aacctcacgt gctgcgaaat
     5401 catcggtaca aatagggcta tatgccgcgt cttttctggc taattttatg aaaagatatt
     5461 tattggcggc acaaaataaa gaacaatttt gaattcctta cattcctggc tattgcacaa
     5521 ctgaatttaa ggctctatta ttacctcaac aaaccacccc aatataagtt tgagattact
     5581 acaatgagcg aagcacttaa aattctgaac aacatccgta ctcttcgtgc gcaggcaaga
     5641 gaatgtacac ttgaaacgct ggaagaaatg ctggaaaaat tagaagttgt tgttaacgaa
     5701 cgtcgcgaag aagaaagcgc ggctgctgct gaagttgaag agcgcactcg taaactgcag
     5761 caatatcgcg aaatgctgat cgctgacggt attgacccga acgagctgct gaatagcctt
     5821 gccgccgtta aatctggcac caaagctaaa cgtgctcagc gtccggcaaa atatagctac
     5881 gttgacgaaa acggcgaaac taaaacctgg actggccagg gccgtactcc agctgtaatc
     5941 aaaaaagcaa tggatgagca aggtaaatcc ctcgacgatt tcctgatcaa gcaataatct
     6001 tttgtagatt gcacttgctt aaaatcccgc catcggcggg attttttatt gtccggttta
     6061 agacaattta ataagaataa gctataaaaa aacggcgtcg attgctcaac gccgtttcgt
     6121 ggataacacc gatacggatg ttacttctta atgcccatct cttcttcaag ccaggcttta
     6181 aattccgtgc caagggtgtt atgacgaata ccgtattcaa cgaaggcctg catgtaacct
     6241 aatttattac cgcagtcatg gctcttccct ttcatatgat aggcttcaac cgtttctttt
     6301 tcgattagca tatcaattgc gtcggtgagc tgaatttcat caccagctcc cggaggggtt
     6361 tttgccagca acggccaaat atccgcacta agtacgtaac gacccacaat agcgagatta
     6421 gacggcgcaa catccgcttt tggtttttca accacgccaa ccatcggtac gctttcaccc
     6481 ggcgctaatt caacgccttt gcaatccaca acgccatatg cggtcacatc agcaaccggt
     6541 tcaaccatga tctggctatg acccgtttca tcgaagcggc ggatcatctc tgccaggtta
     6601 tcctgtgaca aatcggattc atattcatcc agaataacgt caggcaaaat aacagccaca
     6661 ggttcatcac ccactaccgg gtgagcacac aataccgcgt gtcccaggcc tttcgccagc
     6721 ccctgacgaa cttgcataat agttacgtgc ggcggacaaa tagactgcac ttcatcaagc
     6781 agttgacgtt ttacacgttt ttccagcatt gcttccagtt caaaactggt atcaaagtgg
     6841 ttttcaatag agtttttaga tgagtgtgta accagcacaa tttcagtaat gccagccgca
     6901 atacattcat tcacgacgta ttgaattaat ggcttatcga caagtggcag catctctttc
     6961 gggatggctt tcgtcgccgg caacatcctg gttcctaatc ccgcaacggg gataacggct
     7021 tttttgactt tcgtattaat ggcagccatt taaattctcc tggactgttc gtgttttgaa
     7081 cgtgttcata tttctgtatc gcatcccagt atatcagcac cccggagggg ctaagttctg
     7141 aaaaggatgc gagttagcat aattaagaca attacttata cgaatggcga cgatagtacc
     7201 accagccgac attagctggt aatgcaaatt tagcccgcgt tatcgtttgc tcattctgca
     7261 gacaacatca agcgcagtcg accaccggtt ccccatattt ggcattgcca ggcatcgcat
     7321 cgctggctca attgattcaa ataagcgtta cctaaagtgc ctaacggaac accattactg
     7381 atttgcacct ggtgttcgcc ggtatttaac gtcgcattca gacccgcaga aaccagaatg
     7441 agatttttca gttcgcgatg ataatagcca actaatagcg gaaactgccc cggcagattg
     7501 gcctgacgga gtaaatggtt tacctgcttc aataacgcgc ccaactctgg caaccgttga
     7561 ttttggtgtg caagctgttc ctgtaataat ccgttaaaca atgcgcgtaa taacaaggca
     7621 gcaagtacgc cattatgtcc agctcgggtt acatcaaggc aataaaatgc cagatcgttt
     7681 tccgaaagtg cggcaatatc aagcaccagg ccgggtttgt ccgcggcaac caattgacgg
     7741 taattaaccc ggcaatggga aatcacctgc tgaaccggcg gttgtagttc ctgtaataat
     7801 ttcgccgctg cggcagggtt atcaaccatt gcatcccagt cgcgaaaaag cctttcctct
     7861 tcctcaacgc gcgaattaaa catgctggga tagagacagg caaaaaccat ctcgcgcaag
     7921 cgattcagat ctttaactgg tttcagcaaa acatcttcaa cgcccagacg taacgctttg
     7981 gcaatatctg ccatattttc agtggcagat atcaccagaa ctggggtctg gtcgcctctg
     8041 ttacgtatat gctccagcag tttaagcccg ttcattcgtg gcatcgcgat atcacatatc
     8101 atcaggtctg gagtgaaacc tcccagcaac tcaagggcat ccaccccatc agccgccagt
     8161 accgttgtcg ctcccaatga ggaaaaccac gaatccagaa gcgagcgaaa tacctgctca
     8221 tcttcaacaa tgagaatctg ttttccgacc aatggctgcg tcatgttctc tcccctgact
     8281 ggctttactc aatagtggca tgctattgcc acctgcgcct gtcagaatta acttaagtgt
     8341 agataaaaat tctggtcaaa tgttggtgcg taccaacggc aaaagttcgt ccattttctt
     8401 ttccactgcc agctgcccgg ccgcaatggc agcgtgcgcg cgatggaaat caagcgtaga
     8461 tatttgcggg caaacaggtt gaatcagaat atcgggcgga tcacctgcca tgcggttcct
     8521 tttaaggcgg ttctccagca cctggataga agtggtcata atctctgttg ccgttggtgc
     8581 tgtcaccgcc cgacgtgtcg ttatactgcc taacctttct ttcagacgcg catgccacgg
     8641 cagagaatca ccattctcgc tattttcttc actgacatta aaggagagca aatcttgttg
     8701 catcaaatga gcatcgtgct gcaggtcgac cgctatcaca atatcagcac ccaatgcacg
     8761 cgtgagcgaa ataggaattg ggttaacgac tgccccatca accagccagt agccgttatg
     8821 cgcaacgggt gccattagtc ctggaatact gcatgatgcg cgaatagcaa gatggagatc
     8881 gccttcagta aaccataatt cacgcccagt acttaaattg gtggcaacag ccgcgaagcg
     8941 acgggaacaa ttttcgatct ctgtttccgg cattatttcg cggtattgat tgaagacacg
     9001 ctcgccgcgc agtaacccac cgcgctgcca ggagagatcc atcagtcgta aaacatccca
     9061 ataactgaaa gaggtcaccc aatcttccag cgcagataat cgatcgcatg cataggccgc
     9121 gcccaccagc gaaccaattg aacatcctgc aacgatatca atttcaatac ccactttttt
     9181 tagcgcatta ataacgccaa tatgcgacca acctctcgcc gcgccagatc ccagcgccag
     9241 ccctattttt atctttctca ttattcccgc aagattcccc tggaatgcaa tcgtagccac
     9301 atcgctactg ctcggttaac atagtgctac tcaggcggtt aatacgccgt tattgtttcc
     9361 agggagatta tttgtgtctc agctttgtcc ctgtggtagt gctgtcgagt atagcctatg
     9421 ttgccaccct tatgtgtctg gtgaaaaggt tgcacctgat ccagaacatc tcatgcgttc
     9481 gcgttactgc gcttttgtga tgcaagacgc agattattta ataaagacct ggcatccatc
     9541 ttgtggagca gcggcgttac gtgccgaatt gattgccgga tttgcgcata ctgaatggtt
     9601 gggattgacc gtatttgaac attgttggca ggacgccgac aatatcggat ttgtcagttt
     9661 tgttgctcga tttaccgaag gtggcaaaac aggtgcaata attgaacgtt cgcgattttt
     9721 gaaagaaaac ggtcaatggt actatattga cggtacacgt ccgcagtttg gccgcaacga
     9781 tccctgccct tgtggttcag gtaaaaaatt taaaaagtgc tgcggccaat aatggttgac
     9841 ggtacggttt agcaaacact ctcaacaagg tttttccagc aatgcattca ctccaacgta
     9901 aagttctgcg tactatttgt ccggaccaaa aaggtctgat cgcacgtatt accaatattt
     9961 gctacaagca cgagttaaat atcgtacaga acaatgaatt tgttgatcac cgtaccgggc
    10021 gcttttttat gcgcacggaa ctggaaggga tttttaatga ttccaccctg ctggcggatc
    10081 tcgatagcgc attgccagaa ggctccgtgc gtgagctgaa tcctgccggt cgtcgccgga
    10141 tagtgattct ggtcactaaa gaagcgcatt gccttggcga tttgttgatg aaagccaatt
    10201 atggcggcct ggatgtcgaa atcgcggcag tgattggtaa ccacgatact ttacgttctc
    10261 tggttgagcg ttttgatata ccgtttgagc tggtaagcca tgaagggtta agccgcaacg
    10321 agcacgatca aaagatggcg gatgccattg atgcttatca acctgactac gtggtgctgg
    10381 cgaagtatat gcgggtatta acaccggaat ttgtgtcacg cttcccgaat aagatcatca
    10441 atattcacca ttccttcctg ccagcgttta tcggcgcacg tccttatcac caggcctatg
    10501 aacgtggcgt gaagattatt ggcgcaaccg ctcactatgt gaatgacaat ctggacgaag
    10561 gcccaatcat catgcaggac gttattcatg tcgatcatac ctacacagct gaagatatga
    10621 tgcgcgcagg tcgtgacgtc gagaaaaacg tcttaagtcg cgcgctctac aaagtactgg
    10681 cgcagcgcgt ctttgtttac ggtaatcgga cgattattct ttaatcgcca gcaaaaataa
    10741 ctggttacct ttaatccgtt acggatgaaa attacgcaac cagttcattt ttctcaacgt
    10801 aacactttac agcggcgcgt catttgatat gatgcgcccc gcttcccgat aagggagcag
    10861 gccagtaaaa agcattaccc cgtggtgggg ttcccgagcg gccaaaggga gcagactgta
    10921 aatctgccgt catcgacttc gaaggttcga atccttcccc caccaccatg attcaccaca
    10981 gcgatgtggt aacttcggtg agattgtgaa agtttgcact catcggggaa gggtgagaac
    11041 cttcgactaa ggttcgactc gagcgaaagc gagagaacgt tttcacaggc aacggcccga
    11101 agggtgagga gcgaagcgac gaataatcct ttccccaccg ccatctcaag cattacctca
    11161 aaatttgaat tacccctggt ggggttcccg agcggccaaa gggagcagac tgtaaatctg
    11221 ccgtcatcga cttcgaaggt tcgaatcctt cccccaccac catcactttc aaaagtccct
    11281 gaactctcaa gcgaatccgt aaataaatat tctgcccaag cggagaagga tgagaagctt
    11341 cgactaaggt tcgactcgag cgccagcgag agagcgttgc cgcaggcaac gacctgaagg
    11401 gcgaagcgcg cagcgctgag taatccttcc cccaccacca tcactttcaa aagtccctga
    11461 actctcaaac gaatccgtaa ttaaatattc tgcccaagcg gggaaggatg agaagcttcg
    11521 accaaggttc gacttgagcg ccagcgagag agcgttgccg caggcaacga cccgaagggc
    11581 gaagcgcgca gcgctgagta atccttcccc caccaccatc gcttcctgaa ataatcaaaa
    11641 cctgttactc cgagctaatt ttgcccaatg gcaccacgtt taccggacct acaatatcct
    11701 cccttgtagg tcggataagc gaagcgcatc cgacaactca ccccgcaccg tgcccataaa
    11761 aaaaccccgc cgaagcaggg tctatgtgaa ctaaaattcg cttagtgacg agcgcgcacc
    11821 agctggtact tacgtgtcag atactccacc ggtacgctcc agatgtgtac cagacgcgag
    11881 aacgggaaca gcaggaataa cgtcatcccc agcaccaggt gtagacggaa gataaacgcc
    11941 acaccatcga ggtgttgaga agcgccaccg tggaaggtca ctaccgactg cgcccagcca
    12001 accagtttca tcatctcgct accgtccata tgttgggcgg agaacggaat ggtcaacagg
    12061 cccagcgcgc actggataac gagcagcgac aggatcagta tatccgctcc ggtagtcgtt
    12121 gcacgcacgc gtgggctgaa cagacgacgt ttaagcaaaa gcacgccgcc aatcagacac
    12181 agcacgccgc tggcaccacc agcaaacatc gccattttct gtttcacttc aatcggcagc
    12241 caggcttcat acatccagtg cggcgtcaac ataccgaaga agtgaccgac aaaaatcccc
    12301 agaataccga tatggaacag gttcgacgcc aggttcatcc ctttgcgatc cagcatctgg
    12361 ctggacgccg cgcgccaggt gtactgcccg tagtcataac gcagccagct accaatcagg
    12421 aagaccgccc cggctatgta cgggtagata tcaaagaaga acatattcag gaattgcatt
    12481 agtgctgtcc tccggtggtg atattcagat attgcggcgc gacggcaccg gcaaagcgac
    12541 gctgatgagc agtgattgct gaatcaccgc agcctttgtc agcaaagaat ttaacctgct
    12601 cttcttccca aacagcatcc agcgcctgcg gcgtatcatc gcgcgcttcg tcggcaattt
    12661 tttccgccac tttgtcgctg tcgatagcgg tattggccaa tttcagcagc agatcaaaca
    12721 gcacggcata acggctttca cgctgttgca gacgcgcact cagcaatgcc agaatcggcg
    12781 cgatatcttt caaaccttcc acggcttcgc tttgcggcag ctgcgccagg tactccagat
    12841 acagcggcag atgatccggc aattcgcggc tgtttaactg caagccgtgc tgctcgtact
    12901 gcgccagcag gtccaccatc gcctggccgc ggtcgcggga ttcgccgtgc acatgttcaa
    12961 acagcaacag tgacgtggcg cggccacggt cgaacagttc gctgtactgc gcctgggcat
    13021 cgagcggatc catcgtcgtt aaatcgcgca ggaaaatgcc cagcgcatgg gcatcctctt
    13081 ttggcagatt tttcgacgcg gcaatcgcct caaacatctc ttgttgatgc tgccataagg
    13141 cagcatccgg atattcaagg agacgcgata caatcacgag ttcgatcatg gatgcggctc
    13201 cgttttgctg gtcacatcga tggcatcgat acgacggctg ttgaacagat tgaatttggt
    13261 atctgaaccg tggcagccat caccaaaggt aaagccgcag ccatttttct ccgggaaggc
    13321 ttcccgtgcc agttcacgat gactactcgg caccacaaag cgatcttcgt agttagcaat
    13381 cgccagataa cggtacatct cctgtgcctg ggcttcggtc agaccgacct cttccagcgc
    13441 acgggtatca actttaccgt caacggtttc agcacgtttg taatgacgca tcgccagcat
    13501 acgtttcagc gcgcgcagta ccggtttggt atcaccggcg gtcagcagat tcgccagata
    13561 ctgtaccgga atacgcaggc tttcgacgtc tggcagaatg ccgttgctac ccagctcacc
    13621 cgcgtctgct gcagactgaa tcggagagag aggcggcacg taccagacca tcggcagtgt
    13681 gcgatattct ggatgcagcg gcagcgccag tttccattcc attgccattt tataaaccgg
    13741 cgactgctgt gcagcttcaa taacgctcag cggaataccg tctttaatcg cctgctcgat
    13801 gactttcgga tcgttcggat cgaggaacac ctccagctga cgctggtaga gatctttctc
    13861 gttctcggtg ctggctgcac gttcaatcgc gtcggcatcg tacagcagca cgccaagata
    13921 acggatacga ccgacacagg tttctgagca cacggtcggc tgacccgctt caatacgcgg
    13981 atagcagaag atgcacttct cagacttacc gctcttccag ttgaagtaga tttttttgta
    14041 cgggcatccg gtgatgcaca tacgccagcc acggcattta tcctggtcga tcaggacgat
    14101 gccatcttct tcacgcttgt aaatcgcacc gctcgggcag gtcgccacac atgccgggtt
    14161 caggcagtgt tcgcacaggc gcggcaaata catcatgaag gtgttttcga actggctata
    14221 catcgccttc tggatgttgt cgaagttctt gtctttcgcc agtttgtcaa actcaccgcc
    14281 caggtcatct tcccagttcg gccctttttc gattttcgcc atccgttccc cggtaatcag
    14341 cgaacgcgga cgggcaatcg gctgcgattt gctgccttcc ggcgcggtat gcaggttctg
    14401 atagtcaaaa tcgaacggct cgtaataatc gtcgatcccc ggcagatgcg ggttagcgaa
    14461 gattttaccc agcagcatgg cacggttacc catgcgcggt tgcagtttgc cgttgatttt
    14521 acggatccag ccgcctttgt atttttcctg gttttcccag tcagtcggga agccctggcc
    14581 cggcttggtt tccacgttgt tgaaccacgc gtattccacg ccttcacggc tggtccagac
    14641 gtttttacag gtaactgaac aggtgtggca gccgatgcac ttatcgagat tcagcaccat
    14701 gccgacttgt gaacgaattt tcattttacg ctctcctgta cctgatcatt gccttcgcca
    14761 tctaaccagt caatgttctt catcttacgc actacaacaa actcatcgcg gttagaacct
    14821 acggtgccgt agtagttaaa gccgtatgcc agatgggcat agccgccgat catatgcgtc
    14881 ggtttcggcg tgatacgggt gaccgagtta tggataccac cacgctgttg ggtaatttcc
    14941 gaaccaggca ggttaacgat acgttcctgc gcgtggtaca tcatggtcat ccctgccgga
    15001 acacgctggc tgacaaccgc acgggcagtc agagcaccgt tgctgttgaa tacttcaatc
    15061 cagtcgttat cggcgatacc cagatctttg gcatcggctt cactcaacca gaccaccgga
    15121 ccaccgcgac ctaaagtcag catcagcagg ttgtcgctgt aggtggagtg gataccccac
    15181 ttctggtgcg gcgtcaggaa gttgagcgct ttttccgggt tgccgttgga tttctggcct
    15241 atcacttctt tcaccgaacg ggtgtcgatc ggcggacgat aaaccagcag gctttcaccg
    15301 aaatcacgca tccactggtg atcctgatac agttgctgac gaccagagag cgtacgccat
    15361 gggatcagct cgtgaacgtt ggtgtaaccg gcgttgtaag aaacgtgttc atcttccaga
    15421 ccagaccagg tcgggctgga gataattttg cgcggctgtg cctgaatatc gcggaagcgg
    15481 atcttctcgt cttctttatt cagcgccaga tgcgtatggt cacgaccggt aaattcgctc
    15541 agggcagccc aggctttcac ggctacctga ccgttggttt ccggtgccag tgtcaggatc
    15601 atctctgccg catcaattgc ggtgttcagc atcggctggc ctttcgccgg accttccgct
    15661 ttggtgtagt tgagcttacg cagcagatcc atctcgctct gggtgttcca ggcaatccct
    15721 ttaccgccgt taccgatttt ctccatcagc gggccgatag aggtaaagcg ttcgtaagtt
    15781 gccggataat cgcgctctac gaccataatg tgcggtgcag ttttgcctgg gatcaggtcg
    15841 cattcgcctt ttttccagtc tttcacatcc agcggctgcg ccagttcagc ggcagagtcg
    15901 tgctggatag gcagcgtgac gatgtcggtt tctttaccca gatggccgac gcacacttcg
    15961 gagaatttct tcgcgatggc tttgtagatt tcccagtcgc ttttcgcttc ccaggccgga
    16021 tcgaccgccg cagacagcgg gtgaataaac ggatgcatat ccgaagtatt catgtcgtct
    16081 ttttcgtacc aggtcgccgt cggcaaaatg atgtcggaat agagacaggt gctcgacaga
    16141 cggaagtcca gcgtaaccac cagatccagc ttgccttcca gaccattgtc ctgccagtcc
    16201 acttcttccg gcttcacgcc gccctgctgc cccagatctt taccctggat accgtgctcc
    16261 gtccccagca ggtacttgag cataaactca tgacctttac cggaagaacc gagcaggtta
    16321 gaacgccaga taaacaggtt acgcgggtgg tttttgccgt tttccggctg ttccgcagcg
    16381 aaacggatag aaccctcttt cagggatttc accgtatagt ccaccggatt catcccggct
    16441 ttttctgctt cgccagcgat agtcagcggg ttagtgccta actgcggcgc agacggcagc
    16501 cagcccatgc gttctgcacg gacgttaaag tcgatcagat gtccggtata gcgggattta
    16561 tccgccatcg gcgacagcag ttcttccgca gtcacggttt cataacgcca ctggctggag
    16621 tggttataga aataagaggt gctgttcatg tgacgcgccg gacgctgcca gtcgagggca
    16681 aacgccagcg gctgccagcc ggtttgcgga cgcagttttt cctgacctac atagtgcgcc
    16741 cagccgcccc cgctctgacc gacacagccg cagaaaatca gcatgttgat cagaccacga
    16801 tagttcatat cgaggtgata ccagtggttc agccccgcac cgacgataat catcgaacga
    16861 ccgtgcgttt tatcagcgtt atcggcaaat tcacgggcga tgcgaataat ctggctgcga
    16921 gaaacgccgg taatctgctc ggcccaggcc ggggtataag ctttcacatc gtcatagctg
    16981 gttgcacagt taacgtcgtt caggccacgt tccagaccgt agtttgccag cgtcagatca
    17041 taaacggtgg tcaccagggc ggtgctgcca tcagccagtt gcaggcgttt caccggcagt
    17101 ttgtgcagca gcacgttttc cagttccact ttgttgaagt gttccgtgcc gtcgccacca
    17161 aagtacggga agcctacctc agcgatctca tcctggctac ccagcaggct cagttgcagc
    17221 tcggtttctt cgccagtttt gccgtcgcgc tgctcaagat tccatttgcc cttctcgccc
    17281 cagcggaagc caatagaacc gttcggcgca accatttcgc cattggtatt aaaggcgaca
    17341 gttttccatt ccggattgtt ttcctggccc agcgcatcaa ccagatcagc agcgcgcagc
    17401 atacgacctg cagcgtagta accgtcgcgt tcttccagca tcaccagcat cggcatgtcg
    17461 gtgtagcgac gcacatagtc ggtgaaatac tggcttgggt tgtcgagatg gaattcacgc
    17521 agcattacgt ggcccatcgc cagcgccatt gccgcatcgg tgccctgttt cggtgccagc
    17581 cacagatcgc acagtttggc gatttcagcg tagtctggtg tgacggcaac agttttggtg
    17641 cctttgtaac gcacttcagt aaagaagtga gcatctgggg tacgcgtctg cggcacgttt
    17701 gaaccccagg cgatgatgta gctggagttg taccagtcag cagattccgg tacgtcagtt
    17761 tgctcgcccc aggtttgcgg agacgcagga ggcaggtcgc agtaccagtc gtagaagctt
    17821 aagcaagtac cgccaatcag cgagagatag cgtgcacccg atgcgtaaga aaccatcgac
    17881 attgccggaa ttggcgagaa accagcaaca cggtccgggc cgtagttttt gatggtgtaa
    17941 acgttagatg cggcgatcag ttcgttcacc tcctgccagg aagaacgaac aaaaccaccg
    18001 cgtccacgcg cctgcttaaa gcttttcgct ttatcggcgt cttcaatgat agaagcccat
    18061 gcctcaaccg gatcgctatg cagcgccttc gcttcacgcc acattttcat caggcgtttg
    18121 cgcatcatcg ggtatttcag gcggttggca ctgtaaagat accaggagta gctggcaccg
    18181 cgcgggcagc cgcgaggttc atggtttggc agatccggac gggtacgcgg atagtcagtc
    18241 tgctgggttt cccaggtgac cagaccgttt ttgacgtaga ttttccagct gcaggagccg
    18301 gtgcagttta ccccgtgggt agagcggacg attttgtcat gctgccaacg ctggcgatat
    18361 ccatcctccc agtcacggtt ggtattgaga agctggccat gcccatcggc aaaggtttca
    18421 cccttctgtt tgaagtagcg aaaccggtcc aggaatttac tcatcggttt tctcctgtgg
    18481 gagcctgtcg gccctctgat aaatcgacat tgctaatgtt taagggcaag gtaacgctct
    18541 gaaacagcgt gggaattgat aacgatcaag agtgatgggg atgaaaaata aagtaaattc
    18601 ctaagatacc cctttaggat aattaacagt tacagcataa catgctgtaa attaagacgt
    18661 tttatacctg tagttattta aactattaag atttttatgc aaatgggtat taaggagtat
    18721 tccccatggg taacatatta atggttctta atgaaatccg gcgaaataaa ccatataaca
    18781 ttagtatgtt gtaactaaat gacgcataaa aaaagcgcga catcatgccg cgcaaaggat
    18841 aatcaaatat taagatttat tttttagaat gccgaccata taccgcccag gtaatcacta
    18901 cgcaggcgat atagaaaatc aaaaatactt tcattgcgcc gactggcgaa cccgttaatg
    18961 ccagcgagct accgaacgct ttcgggataa agaagccacc aatcgcgcca atcgcagaga
    19021 tgaaacccaa cgccgccgcc gtgtcggttg ccgcttcacg catcgcacgt tcgtcagaac
    19081 ccccttctgc tttcacgcga tccattgtca gtttacggaa gatcacggaa atcatctgga
    19141 aagtggaacc actacccagc ccagctgtca ggaacagcgc caggaagacc gcgaagaacg
    19201 ccatgaagct tccgccctgc ccgtcagtcg gtaaggtcag gaacagcagg ccgctgaaaa
    19261 tcgccatcag aataaagttc accagcgtga cacgagttcc gcccagacgg tcagataatg
    19321 caccacctgc agaacgcgcc agcgcaccaa taaacggccc gaagaaagcg tattgcagaa
    19381 tctgaacatc cgggaactgc gtttttgaca gcatcgcaaa gcccgcggag aagccgatga
    19441 aggaaccgaa ggttgccaga tacagcaggc tcataatcca cagatgaccc cgtttgagta
    19501 ccggcaactg ctccttgatg gaggctttcg aggtagcaag atcgttcatg ccaaaccacg
    19561 ccgcaatggt gaagatggca aggaacggca cccatatcca ggacgcattc gccagataca
    19621 gctcagtccc atccggctgt ttgacgccct ggctaccaaa tactgcgaaa atcgacagtg
    19681 ataccaccag cggagcaacc aactgcataa cgctaacgcc catgttgccc agaccaccat
    19741 tcaggcccag cgcgccaccc tgcttctgtt tcggaaagaa gaagctgatg tttgccatac
    19801 tggatgcgaa gttcgcgcca gcaaagccgc acagcagaga gatgatgatg aagacgctat
    19861 aaggcgtgga ggtatcctgc acggcaaaac ccagccagac acaaggaata atcagaatac
    19921 cggtgctgaa cgccgtccag cgacgaccac cgaagatagg aaccataaag gagtatggaa
    19981 cacgtaataa cgcgccagaa accgaaggca acgcagtcaa cataaatagc tgatcggtcg
    20041 taaaattaaa accgactttc ggtaagttca ccgcaacagc gctgaacaac atccatacgc
    20101 aaaacgccag cagcagacag ggaacggaaa tccacaggtt gcggctggca atacgttgac
    20161 cgcgttgttg ccagaacgca ggatcttccg gtcgccaatc tgtaatgaca gctccagtag
    20221 ccctttcggg ggcggatgag tgactcatag acacctctga tactcgtttc gatgtctgcc
    20281 accttagtgt ctgtagctaa aggcaatttg atgtaaatca aacgataaga taactttatc
    20341 tatcattgat atttatcatt acccatagtg agtacagtga tttcataaaa attatgagat
    20401 ttttcacggt gctgtaaaat ccctaccctt accgatgtaa agctactaac cacacggcaa
    20461 ataaggagta actctttccg ggtatgggta tacttcagcc aatagccgag aatactgcca
    20521 ttccagaatg tatcgtcaca ttcattaagg ttattgctca tttaaagcct gaaggaagag
    20581 gtttacatgc ttaaacgttg tctctctccg ctcaccctgg ttaatcaggt tgcgcttatt
    20641 gtgttgcttt ctactgctat tggactggca gggatggcgg tttctggctg gctggtgcaa
    20701 ggcgttcagg gcagcgccca tgcgatcaac aaagcgggat cgctgcgcat gcaaagttac
    20761 cgtctgttgg cagcagtgcc attaagcgag aaagacaagc ccttaattaa agagatggaa
    20821 caaacggcat ttagcgccga gttgactcga gcagcagaac gagacggaca actggcgcaa
    20881 ttacagggtt tacaagatta ctggcgtaat gaactcatcc ctgcactgat gcgtgcacaa
    20941 aaccgcgaaa cggtgtcagc ggatgtcagc cagtttgttg ccgggcttga tcagctggta
    21001 tctggttttg accgcaccac ggaaatgcgc attgagacag tggtactggt ccatcgggta
    21061 atggcggtat ttatggcact tttactggtg ttcactatta tctggttgcg ggcgcgactg
    21121 ctacaaccgt ggcggcaact gctggcaatg gcgagtgccg tcagccatcg cgattttacc
    21181 caacgcgcaa acatcagcgg gcgcaacgaa atggcgatgc ttggaactgc gttgaacaat
    21241 atgtctgcag aactggccga aagttatgcc gtacttgagc agcgggttca ggagaaaacc
    21301 gccgggctgg agcataaaaa tcagatcctc tcttttttat ggcaggctaa ccgccgtttg
    21361 cattcccgcg ccccgctgtg tgaacgcctg tcacctgtac tcaacggctt acagaattta
    21421 accctgctac gtgatatcga actgcgggtg tatgacactg atgatgaaga gaatcatcag
    21481 gagtttacct gccagccaga tatgacttgt gatgataaag gctgccagct ctgcccgcgc
    21541 ggcgtattac ccgttggtga tcgcggcacg accctgaagt ggcggctggc tgactctcat
    21601 acgcagtacg gtattttgct ggcgacccta ccgcaggggc gtcatcttag ccatgatcaa
    21661 caacaactgg tggataccct ggttgaacaa ctcaccgcca cgctggcgct ggatcggcat
    21721 caggaacgtc agcaacagtt gatcgtgatg gaagagcgtg ccaccattgc gcgcgaactg
    21781 catgattcta ttgcccaatc tctctcttgc atgaagatgc aggtgagttg tttacagatg
    21841 cagggcgatg cgctgccaga aagcagccgc gaactgttaa gtcagatccg taacgaactg
    21901 aatgcatcct gggcgcagtt gcgtgaattg ctcaccacat tccgcttgca gctcaccgag
    21961 cctggattac gtccggcgct ggaggcgagt tgcgaagagt acagcgccaa atttggcttc
    22021 ccggtgaagc tggattatca attgccgcct cgtctggtgc cttcgcatca ggcaatccac
    22081 ttgttgcaaa ttgcccgtga ggcattaagt aacgccctca aacattcgca agcgagtgag
    22141 gtcgtggtga cggtggcgca aaacgataat caggtcaaac tgaccgtcca ggataacggc
    22201 tgcggcgtgc ctgaaaatgc catccgcagc aatcactacg gcatgataat aatgcgcgac
    22261 cgtgcgcaaa gtttacgagg cgattgccgc gtccgccgtc gtgaatcagg tggcaccgaa
    22321 gtggtggtca cctttattcc cgaaaaaact ttcacagacg tccaaggaga tacccatgag
    22381 taatcaggaa ccggctacta tcctgctgat tgacgatcac ccgatgctgc gaactggcgt
    22441 aaaacagctt atcagtatgg caccagatat caccgtggtt ggcgaagcga gtaatggcga
    22501 acagggtatt gaactggcgg aatctcttga tcccgatctg atcctgttag atctcaatat
    22561 gcccggcatg aacggtctgg aaacgctgga taaactgcgc gaaaagtccc tctcagggcg
    22621 cattgtggta ttcagcgtct ctaaccatga agaagatgtg gtcaccgcac tgaaacgcgg
    22681 cgcggatggc tatctgttaa aagatatgga accggaagat ctgctgaaag cattgcatca
    22741 ggcagctgct ggcgaaatgg tattaagcga agcattaacg cctgttctgg ccgccagctt
    22801 gcgcgctaac cgtgccacga ctgagcgcga tgttaaccag ttaaccccac gcgagcgcga
    22861 tattctcaag ctgattgccc agggtttgcc gaacaagatg attgcccgcc gcctggacat
    22921 caccgaaagc acagtaaaag tgcacgtcaa gcacatgctg aagaaaatga agctcaagtc
    22981 tcgcgtggaa gcagcggtat gggtgcatca ggagcgcatt ttctgattac ggttcccagc
    23041 gcagttcgtc gtttgacaat gcgtcgaacg gttcgacaag cgttagcgtg atctcattgg
    23101 aggagacacg ctgcccctgg ttatcttcca ccaccaccga caatcgccag tgattgctcg
    23161 ccccttcccc gttctgccag tcaggcatga tcagcgtcca gccctccgcg ctgttggctt
    23221 gtgcgcccgg cgtcaaactt aatatctgcg tatcgccctg ccaaatcagt tgccgaatac
    23281 cgtaacgact gcggatttgt aatttcagcg gcactgtttc gccaggtttt agatcccacg
    23341 gcggtgtcgc aagaaacacc gttaacgttt ttcgctgtcg gtactcaaga gtcggtagat
    23401 tatttcgctg cggattatca tagcgactac cacgtaacga ctgactttcg gcaacctcgc
    23461 ccgcagaaag ttgttttttg agcggtacac caaagcggta attaagattc agcccgaggt
    23521 tattttgatt ctcgccactt tcaccctgtt tatgctgggc cgtcacagtg actaatggca
    23581 caggggtgta atttaatccc agactcaacg cgacgggatt gtgataaccc gtaccagagt
    23641 taaacaaatc aacacgatca ccaaaatact gttctacgct gacactggta ttgaggtgtt
    23701 gatagaacgg catgcgcatc cgggctgtca ggtcgtaccc gcgcgccatc cgttgttcct
    23761 gcgtggctgt ctgttcatgc catgcagcaa acggctgata aaagtttgcc gatagtcgca
    23821 aatattcgcc ccacgcttcg gcaccaaagc ccgctcgctg aagattttca tccagcaagt
    23881 tgtcataaaa agtgttataa cccaccagcc agttgccgcg cgcccagcgt tgcccaacgc
    23941 ccacattgct caccagccca tcatcctgct gagtaagacc aagctggctc caggtgagat
    24001 aacgatcatt atcttgtaac ggcacaaacc agcttccacg actgccggta aaatgacctt
    24061 cgttatccac tttgacgtca acactggcat ttccccacgg tgatagccac gactctacgt
    24121 gctgattaac ctgttgacta agcgcgtcgc ggacttttcc caatgcgaaa gcttttgcct
    24181 gctcgccagt atccagcccg ttatcattca tactggtttc gccgaaatct ttcacaattt
    24241 cagcaaagtg tttttcccca tcatgatttt caggcgccat gcctaaatcc ggcagaccat
    24301 cattgttatt atcaaaggga tttgccgctt tttgctcgaa ggtagattgt gcgttagctg
    24361 taccgcctgc cgccagcaag agtaaataaa acggaataat gcgaggaacg aaacggctca
    24421 aaacgtcggt aacttccggt aaaaaatgaa aaaacatagc cttaaagaat aacgattact
    24481 ggcaactttt gtcgtatttt ctcgcgcttt caggaaaatc cttaaaaatg tgaccgattg
    24541 gtcagacgct gcagaagcag taagatagaa gatgagtgtc taattccctg aatacaggag
    24601 taaataatgc aaaaaatcgt gatcgttgcc aatggcgcac cttacgggag cgaatccttg
    24661 tttaacagct tgcggctggc cattgcgtta cgagagcagg agagcaatct ggatctgcgt
    24721 ctgttcctga tgtctgatgc ggtcacagcc gggttgcgcg ggcaaaaacc aggggaaggc
    24781 tacaacatac agcaaatgct ggagatcctt accgctcaga atgtaccggt gaaattgtgc
    24841 aaaacctgta ccgacgggcg cgggattagt acacttcctc tgattgatgg ggtggaaatc
    24901 ggtactcttg tggaactggc gcaatggacg ctgtcagccg ataaagtgct cacattttaa
    24961 tgtttgaggg ggcgtctgac gccccacgat gagggaagtt tactcaggcg aatcccggcc
    25021 gcaacacacc atccggataa ttctccgcca gcagtttttt taccgatacc agcaaatcat
    25081 tcagcccatc gtcctgcatt cccagtttga taagctcctg ttccagtgaa aacaggtatt
    25141 gtgcgttggt tcccagcgga ccgctcgccg ccgcaatcaa cggcgcgatg acctgagcgc
    25201 gagtgtcaga ttcatattct ggatgtcgcg ggtccataat aaacacaatg gcgtttactg
    25261 tgcgtccatc atcaagatca agctgacacc aggttggcag ataacagccg gtaatcatct
    25321 cgcgcttcca caacagggtt agttcctgct ccagcgtctc ttctggcagt cgataggcga
    25381 cgcctgtggt acgtccgccc tcttttagtg caagcatccg tcccggctgg tgcgcagtcc
    25441 cgcgcccggc ggtcaggcgc aggcaaaacg cgcgatgcca tccaaccagt gtaccggtgc
    25501 acgactcggt aaactccagt gccggattcc acatcaacga tccataaccg aagatccaca
    25561 ccggtctctc atcaggtcga caagccagcg tcgccgccag cgaagccgcc cgttgttctg
    25621 ctgaccataa gagtgattct tcaatggcac caaatgccgt tttacaatcg gcattcatca
    25681 agaaatcacg cgttatcacc ttcttacacc tccgccactg catgcttccc tgcgacaact
    25741 ttttggtgtc ttccggacat ttttactgct cattttgaaa ccactaacgg gacaatatgc
    25801 aatccgcgga ccacttgcaa ggcaacaccg cacgaatagc tttaactaag ctaccggttt
    25861 tacgattttt tatgccattt atcatcatcc cctttggcat attcatgctt cacagcagcc
    25921 caggccactt tatgcgcggt ttcttcgcga ctggcgtcat cacgccgatc ttctttatct
    25981 ttatattgat cccatgcgct gttgaacgct tctttataga tatcctgggc atgagacggt
    26041 agaacgtgct ttacgctttc cggcaggtcg cttttcgttt tatacggcat cgttgactcc
    26101 ttttacaaag ttgaacatta agtgtggtca acaatatcta ctcttgcaaa aacataagaa
    26161 ttttcatgtc agactgttct taagaaacgc taaaacaaac attgcctaaa cgtaatactt
    26221 atttttatgc cccaatgagt aaaaaatcga caacggggta aaaattcgta aaatgttacc
    26281 gatatccaga cactttctgt tatttttcct acctattttt tacattttcg ataaattcat
    26341 ctgcaaaggg ataaccataa tgtcaaatgc tcaagaggcg gtaaaaaccc gccacaagga
    26401 gacttcgctt attttcccgg ttctggcgct ggtagtgctg ttcctgtggg gaagcagcca
    26461 gacactacca gtggtcattg ccatcaatct tcttgcgctt attggtattt taagtagcgc
    26521 ctttagtgtt gtccgtcatg cggacgtatt agcccatcgc ctgggagaac cttacggttc
    26581 gcttattctt agcctttcag tggttattct tgaagttagt ttgatttcag ctttaatggc
    26641 aaccggcgac gccgcgccaa cgctaatgcg tgatacgctc tattcgatca ttatgattgt
    26701 taccggtggg ctggtcggct tttcattatt gttgggcggt cgtaagtttg ccacccaata
    26761 tatgaatctg tttggtatta agcagtattt aattgcgctg ttccccctgg cgataatcgt
    26821 actggtattt ccaatggctc tgcctgcggc gaatttttca accggtcagg cgttactggt
    26881 agcattaatt tctgcggcaa tgtatggtgt atttttgctg atccagacca aaacgcatca
    26941 aagtttgttt gtctacgagc acgaagatga cagtgatgac gacgacccgc accacggtaa
    27001 accgtctgcc catagcagcc tgtggcatgc tatctggttg attatccacc tgattgccgt
    27061 tattgcagtc accaaaatga acgccagccc gctggagact ttgctcgaca gcatgaatgc
    27121 ccctgtcgcc tttactggct tcctggtggc actgttgatt ctgtcgccgg aaggtttagg
    27181 tgcattaaaa gcagtgttga acaaccaggt ccagcgcgcg atgaatctgt tctttggttc
    27241 agtgttagca acaatttcgc ttaccgtacc tgtcgtcacg ctaattgcct ttatgacggg
    27301 taacgaattg cagtttgcac ttggtgcgcc agaaatggtg gtgatggtgg cctctttagt
    27361 gctgtgccat atctccttct ccaccggacg tactaacgtg ctcaatggcg cagcgcatct
    27421 ggcactgttt gccgcctatt tgatgacgat atttgcctga cagttatatc tcctccgatg
    27481 acggaaaccg ggggagataa tataaattcc tgcttattgt atttcttcat actacctgga
    27541 caggtcgtta atcagaattg cgagcctgta ccacaacctg cacgaaacca ctgcaacagc
    27601 tgttgaaaag ccggaatgta ctctttattg caaacatttc agggatgaag tgaagatgaa
    27661 ttgagacgta gtatccacac caagccggcg ttatccggtg aggcgcaatg ttgcgggggt
    27721 tttatccctg gtggcattgg ttgctggaaa gagaaaaccc ccgcacgttg agaggtaaaa
    27781 cctgacaaca gggctaatct tgaaaccaga cacttcaaga atagccgcaa aatgttgtca
    27841 ttacaacaag gcggctatat gacgctcgcg cagtttgcca tgactttctg gcacgacctg
    27901 gcggcaccga tcctggcggg aattattacc gcagcgattg tcggctggtg gcgtaaccgg
    27961 aagtaaggtg aaaacgggcg tcaggctgcc gtgatggcaa tatgcgcccg aaaccggccg
    28021 caggggtaac agcctgcggc catttttgtc tttatcactt ccacaagtaa atattggcaa
    28081 cccgagctgg aagcatcatc cagagcactc atgaaacgac tgtaacagct gttgaaaaac
    28141 cggaatgcac tcttcattgc aaacatttca gggatgaagt gaagatgaat tgagtcgtag
    28201 tatccacacc aagccggcgt tatccggtga ggcgcaatgt tgcgggggct ttatccctgg
    28261 tggcattggt tgctggaaag agaaaacccc cgcacgttga gaggtaaaac ctgacaacag
    28321 aacgggggct aatcttgaac catcaacaat tcaagaatag ccgcaaaatg ttgtcattac
    28381 aacaaggcgg ctatatgacg ctcgcgcagt ttgccatgat tttctggcac gacctggcag
    28441 caccgatcct ggcgggaatt attaccgcag cgattgtcgg ctggtggcgt aaccggaagt
    28501 aaggcgaaaa cgggcgtcag gctgccgtga tggcaatatg cgcccgaaac cggccgcagg
    28561 gtaacagcct gcggcctttt ttgtatccgg cacccatgaa aaaagtctta acgcagaacg
    28621 ctaagacttt atttttcaag caaaaaagat tacttgctgg tatccagttc ttcgaaacct
    28681 ttcaccagat catcaatcgc tttcatctgc ctgaggaacg gttccagttt agccagcggc
    28741 agcgcggatg gaccatcaca tttcgcatgt tccggatccg gatgcgcttc aataaacagc
    28801 cccgccagac ctaccgccat accggctcgt gccagctcag ccacctgagc acgacgaccg
    28861 ccggaagctg cgccaaaagg atcgcggcat tgcaatgcgt gggtcacgtc gaaaatcacc
    28921 ggcgagttac cagacacttt cttcataatg ctgaagccca gcatatcgac aaccaggttg
    28981 tcatagccga agttagcacc acgatcgcaa agaatcactt tttcgttgcc gccttctttg
    29041 aatttatcaa cgatattacc catctgtccc gggctgacaa actgtggttt cttgacgtta
    29101 attaccgcac cggttttcgc catggcttca accaggtcag tctggcgagc aagaaacgcc
    29161 ggcaactgaa tcacatccac gacatcagca acgggctgtg cctgacttgg ttcgtgaacg
    29221 tcggtgataa ttttcacgcc aaaagtctgc ttcaactcct ggaagatttt catcccttct
    29281 tccaggcccg gtccacgata agagtggatg gaggagcggt tggctttgtc aaaagaggct
    29341 ttgaacacgt aagggatacc cagtttctgg gtcacggtta cgtagtgctc gcaaatgcgc
    29401 atcgccagat cgcgagattc cagcacgttc ataccgccaa acagtacgaa cggcaggtca
    29461 tttgctacgt tgatgtcgcc aatgctaacc actttttgtt tcataggatc gccttaacct
    29521 tgagtaaaat gtcgattaat taatgcagca caatatgttt atgcgcgatg ttatttattt
    29581 gcgcacggat catttcgctg atcgggtctt ccggacactg ttcaacgaaa taacttaaat
    29641 cgttcaacgc aacgtgttcg caatccagtt gcgcataaat caacccgcga tcgcgaattt
    29701 catagggatc ttccgggttg aattgtaata aagcttcgct ggtgcgtaac gccagctcca
    29761 tctgattttc ttccatcaac gaggctttga gtgtatccag caatttgcgg attacctcaa
    29821 tgttatcagc ttcatcaagg tcttcataaa acagttccgc cgacgggctg atatttccct
    29881 ttaaccatac gtccagcata tgttcgctta acgattcacc gttaaaagga ttaatcagcc
    29941 aaatttcgcc atccggacat tcaatgcgca atatcagctg cgtagggaaa atcaccggca
    30001 gcagcggcaa atcgagacga ttcgcgaccc ataataaaac cgcacctaat gataccgcac
    30061 tgccctgtcg attctttaac acctggtcca gccataatgc atcggaaaga cgataaacgc
    30121 cgcgtgaggc tttaaatccc cagtcgccgt aaaacagcgc gatcagtttt tccagctgct
    30181 cttctaaagg cagaagctgg ctgatttctt ccttcgccag actaacgaga cgctccagtt
    30241 cgtcgtaaac atcttgcgag ggaaaatcgc ggcggattgc ttcgcaagcc aggatcatgc
    30301 cttcgcacaa tggcgcttta ttaaattcga aatcagctaa cgatctcatg acttacccca
    30361 gtaacggtac ttttgtggtg gcgagtttaa tgatgatgta cagcaccacc aacgtcagcg
    30421 ggaaggcaat aatgcgcgcc tgctgactgt gcatacgacg ataatcgagt gcaataaaac
    30481 ccaaaacgat ataaatgata actccaaaca gcttttcagt cagccactgt gcctgcccgg
    30541 aaaatggcag gatgtgcgct ttagccatca acgcaatgcc gcttaacaat aacaccgtat
    30601 cgacaaccgg cggcacaatg cgcgtccagc gagcaaatgc ctgtggatgc ttctgataac
    30661 gtagccagaa acgtaaggtt aatagcccaa cagaaagcgc gatactaata agatgaacac
    30721 taagcagtgt agaaaaactt gtcattgata atagcggccg agcgttacgc gctcgttatc
    30781 accatagtca cggcaggttt cgacgtcatg ataccccgcg aggataaatg cctgtcgcac
    30841 cgcttcgccc tgctgccagc catgttccag aagcagaaag ccgccggata ccagcgcgtt
    30901 acgcgactgt tcgatgatat gcacgatgtc tgccattcca ctgtctgccg caaccagcgc
    30961 agtgagcggc tcaaagcgga catcgccttg ttgaagatga gggtcctgct cgtcaatata
    31021 cggcggattg ctgacaatca tcgcaaactg ctgcccggct agcgcgctaa accagtcgct
    31081 ttgcagaatg tggatatttt tgatcgccag atgctgggca ttacgttgtg ccagggcgac
    31141 agcatcgggc atacgatcta cagcggtaat ttcgcagtcc gggcgctcgc gagccagcgc
    31201 aagcgcaatc gccccggtac ccgtcccgag atcgagaata cggcaaggtt gttcaggcaa
    31261 ccgcgccagt gcctgctcca ccagacactc cgtatccggg cgcggaatta aggtcgctgg
    31321 cgaaacaaat aacggcaacg accagaattc tcgcaccccg gttaaatgag caatgggttc
    31381 accatcgcga cgacgtgtca gtagcgcatc aagttgctga cattgttcgt cagtcagctg
    31441 cgtttcacca aaggcgagaa taaaagtacg ccctctgccg gtaacatgct ccagcaggat
    31501 ttcagcatca cgccgcgggc tttcgctcgc ctgaagttgg cttattgctt cacgtaacca
    31561 gtgttgatat tccattattc ctgctcggac aacgccgcca gttggtcggc ctgatgttcc
    31621 tggataatcg gttcaatcag catatccagc ttaccttcca tcacttcatc caggcggtag
    31681 agcgtcaggt tgatgcggtg atcggtaacg cgcccctgcg ggaagttgta agtacggtta
    31741 cggtcgctgc gatcgccact ccccagcagg ttacgacggg tagacgcttc ggcctgttgc
    31801 cgttttgcca tttcagcagc gtggatgcga gcaccgagta cagaaagtgc tttagctttg
    31861 tttttatgtt gtgaacgttc gtcctgacat tcaacaacaa tcccggtcgg caagtgagta
    31921 atacgaattg ccgaatcggt ggtgttaacg tgctgaccac ccgcccctga cgagcggaaa
    31981 gtatcaatgc gtaaatctgc tgggttgatg tccggcagtt ctgcgtcagg cagttctggc
    32041 attaccgcaa cggtacaagc agaagtatga atacgaccct gcgattccgt agcaggaaca
    32101 cgctgcacgc gatgaccgcc agattcaaat ttcagacgac catacacacc atcaccgcta
    32161 attttggcga tgatctcttt ataaccacca tgttcaccct cgctggcgct catgatttct
    32221 acccgccagc ggcgggcttc ggcgtaacgg ctgtacatac ggaacagatc gcctgcgaac
    32281 agcgctgctt cgtcgccgcc ggttccggct cggacttcga ggaaggcgtt acgttcgtca
    32341 tcaggatctt ttggcagtaa cagaacctgt aattgctgtt ccagttgctc gcttttttct
    32401 ttagcttcgc gcagttcatc ctgcgccatc tcacgcattt caggatcatc gagcatcatc
    32461 tgtgcggttt cgatatcttc ctgaacctgt tgccagtcgg taaaacagcg cgaaacatca
    32521 cttaactgcg catattcgcg tgataatgcg cgaaaacgtt cctggtcggc gatagtttgc
    32581 gcgtcaccca gcaacgcctg aacttcttca tggcgttcat gcagggcttc cagtttggca
    32641 acgatagaag gcttcatagg cgtaaatgca ccctgtaaaa aaagaaaatg atgtactgct
    32701 actccagccc gaggctgtcg cgcagaatat tcaggcgttc gttatccccg tcacgggcgg
    32761 cctgttgaag tgatttcgtt ggcgcatgga tcaagcggtt agtcagtttc catgccagat
    32821 cctgcataat ggcttgcgcg tcgccgccct gctcaagggc cgctaacgct ttggcggtta
    32881 actcatcgcg aacttgctct gcctggctgc gatactcgcg aatggtttcg ctggcgcttt
    32941 gtgctcgcag ccacgccata aattcgctgg tttcctgagc gacaatagtt tccgcctcaa
    33001 ccgctgcggc tttacgctgc gccaggttgt gcgaaatgat gctttgcaga tcatcaacgc
    33061 tataaagata agcattcgcc agtttgccaa cttccggctc aacatcgcgc ggaacggcaa
    33121 tatccaccaa cagcattggt tggttgcgac ggctttttaa tgcgcgctcc accatgcctt
    33181 tcccgataat cggtaacggg ctggcggtgg aactgatgat gatatcggct tcgcgcagac
    33241 gttcgtcgat atcactcagg gcaatcactt ccgcgccgac ttcatctgcc agaatttggg
    33301 cacgttcgcg agtgcggttg gcgataatca tcttctgtac tttatgttcg cgcagatgac
    33361 gcgctaccag ctcgatggtt tcgcccgcgc ctaccagcaa cactgtgacc gtagagagcg
    33421 attcaaagat ctgccgcgcc agcgtacaag ccgcaaaagc gacagacaca gcgctggcac
    33481 cgatatctgt ttcagtgcgt acgcgtttcg ctacagagaa agatttctgg aacatgcgtt
    33541 ccagttcgct ggccttcata tgaccttttt gcgaatcggc aaacgctttt ttaacctgac
    33601 cgaggatctg cggctcccca agaaccagtg aatccaggcc gctggcaaca cgcattaaat
    33661 ggctaaccgc gtcgttatcc tgatgccagt agaggctttt acgcagatct tcttcattaa
    33721 gattgtgata atcgcaaagc cagcggatta acgcctcttg caagttgtcc tgctcttcga
    33781 cgctaagata aagctcagtg cggttacacg tcgacagcac caccccgccc tgcaccatcg
    33841 gctgtgcgag cagactgtcc agcgcctgat caagtttgtc cggcgaaaac gatacacgtt
    33901 ctcgcagcga tacaggtgcc gttttatggt taataccgag cgctaaaagg gtcatgtctg
    33961 cgggaaataa taccaacgtt gataaggtta gtctcggcgc atcataccgt atgcgcgaga
    34021 tcaataaaag ggagcgcccc cttttggagt aattgcccga acccgttaga tttctacaat
    34081 taagacaact tgaacataga cgatgactaa tggtgacgct agcattaagg gttataactg
    34141 caacgtatct caaggacttg tcatcactat gcccctgccc gattttcgtc ttatccgcct
    34201 gctaccgctg gctgctcttg tgctcactgc ctgttccgtt accacgccca aaggtcctgg
    34261 caaaagcccg gattcgccac aatggcgtca gcatcagcaa gacgtgcgca atcttaatca
    34321 gtatcagact cgcggcgcgt tcgcttatat ttctgaccaa caaaaagtgt acgcccgctt
    34381 tttctggcag caaaccggcc aggatcgcta ccgtctgctg ctcactaacc cattgggcag
    34441 cacggaactg gagctgaatg ctcaaccggg taacgtgcag ttagtcgaca ataaaggtca
    34501 gcgttatacc gccgatgacg ccgaagagat gattggcaaa ttgactggaa tgccaattcc
    34561 gctcaacagc ttgcgccagt ggattttagg tttaccgggt gatgcaaccg actacaaact
    34621 ggacgaccag taccgcctga gcgaaattac ctacagccag aatggcaaaa actggaaggt
    34681 tgtttatggt ggttatgaca ccaaaacgca acctgcgatg ccagccaata tggaactcac
    34741 cgacggtggt caacgcatca agttaaaaat ggataactgg atagtgaaat aatgcggaca
    34801 cagtggccct ctccggcaaa acttaatctg tttttataca ttaccggtca gcgtgcggat
    34861 ggttaccaca cgctgcaaac gctgtttcag tttcttgatt acggcgacac catcagcatt
    34921 gagcttcgtg acgatgggga tatccgtctg ttaacgcccg ttgaaggcgt ggaacatgaa
    34981 gataacctga tcgttcgcgc agcgcggttg ttgatgaaaa ctgcggcaga cagcgggcgt
    35041 cttccgacgg gaagcggtgc gaatatcagc attgacaagc gtttgccgat gggcggcggt
    35101 ctcggcggtg gttcatccaa tgccgcgacg gtcctggtgg cattaaatca tctctggcaa
    35161 tgcgggctaa gcatggatga gctggcggaa atggggctga cgctgggcgc agatgttcct
    35221 gtctttgttc gggggcatgc cgcgtttgcc gaaggcgttg gtgaaatact aaccccggtg
    35281 gatccgccag agaagtggta tctggtggcg caccctggtg taagtattcc gactccggtg
    35341 atttttaaag atcctgaact cccgcgcaat acgccaaaaa ggtcaataga aacgttgcta
    35401 aaatgtgaat tcagcaatga ttgcgaggtt atcgcaagaa aacgttttcg cgaggttgat
    35461 gcggtgcttt cctggctgtt agaatacgcc ccgtcgcgcc tgactgggac aggggcctgt
    35521 gtctttgctg aatttgatac agagtctgaa gcccgccagg tgctagagca agccccggaa
    35581 tggctcaatg gctttgtggc gaaaggcgct aatctttccc cattgcacag agccatgctt
    35641 taagccgggc aagctgagtt tcggtgacaa cgtcaccttg ttccagacgt tgcatcgcgc
    35701 tctttaatac accgcctgga aaggatcatg cctggcccgc acagttttcg gcagattctt
    35761 tccaccaatg gacgcatgcc tgaggttctt ctcgtgcctg atatgaagct ttttgctggt
    35821 aacgccaccc cggaactagc acaacgtatt gccaaccgcc tgtacacttc actcggcgac
    35881 gccgctgtag gtcgctttag cgatggcgaa gtcagcgtac aaattaatga aaatgtacgc
    35941 ggtggtgata ttttcatcat ccagtccact tgtgccccta ctaacgacaa cctgatggaa
    36001 ttagtcgtta tggttgatgc cctgcgtcgt gcttccgcag gtcgtatcac cgctgttatc
    36061 ccctactttg gctatgcgcg ccaggaccgt cgcgtccgtt ccgctcgtgt accaatcact
    36121 gcgaaagtgg ttgcagactt cctctccagc gtcggtgttg accgtgtgct gacagtggat
    36181 ctgcacgctg aacagattca gggtttcttc gacgttccgg ttgataacgt atttggtagc
    36241 ccgatcctgc tggaagacat gctgcagctg aatctggata acccaattgt ggtttctccg
    36301 gacatcggcg gcgttgtgcg tgcccgcgct atcgctaagc tgctgaacga taccgatatg
    36361 gcaatcatcg acaaacgtcg tccgcgtgcg aacgtttccc aggtgatgca tatcatcggt
    36421 gacgttgcag gtcgtgactg cgtactggtc gatgatatga tcgacactgg cggtacgctg
    36481 tgtaaagctg ctgaagctct gaaagaacgt ggtgctaaac gtgtatttgc gtacgcgact
    36541 cacccgatct tctctggcaa cgcggcgaac aacctgcgta actctgtaat tgatgaagtc
    36601 gttgtctgcg ataccattcc gctgagcgat gaaatcaaat cactgccgaa cgtgcgtact
    36661 ctgaccctgt caggtatgct ggccgaagcg attcgtcgta tcagcaacga agaatcgatc
    36721 tctgccatgt tcgaacacta atcgaacccg gctcaaagac ccgctgcggc gggttttttt
    36781 gtctgtaata tccatttgta tgacctatgc ctccttcacc tgccatttag ttgacagatg
    36841 atgcgctcac ggatgaaaca ttattgtgaa caaaatattt tcctcacatg tgatgccttt
    36901 ccgcgctctg atcgacgctt gctggaaaga aaaatatact gccgcacggt ttacccgtga
    36961 cctgattgcc gggataaccg tcgggattat tgctatcccg ctggcgatgg cgttggctat
    37021 tggtagtggt gtggcacccc agtacggttt atataccgca gctgttgcgg ggattgtcat
    37081 tgctctgacg ggtgggtcac gctttagcgt ttccggtccg actgcggcat ttgtggtaat
    37141 tctctatccc gtttcgcaac agtttgggct ggcaggactg ctggttgcga ccttgctgtc
    37201 ggggatcttt ttgattctga tgggtctggc acgctttggt cgcctgattg agtatattcc
    37261 ggtttccgtc accttaggtt tcacctcggg tatcgggatc accatcggta ccatgcagat
    37321 taaagatttt ctcggtctgc aaatggccca tgtcccggaa cattatctac aaaaagtcgg
    37381 cgcattattt atggcgctgc cgaccattaa tgtgggtgat gctgccattg gcattgtgac
    37441 gctaggtatt cttgttttct ggccgcgtct gggcattcgt ttacccggtc accttccggc
    37501 cttgctggct ggttgcgcgg tgatggggat tgttaacctg ctcggcggac atgttgctac
    37561 catcggttcg caattccact acgtcctggc cgatggttct cagggtaacg gtattccgca
    37621 actgctaccg caactggtgc tgccgtggga tctgcctaat tcagaattca cgctaacctg
    37681 ggattctatt cgcacactgc tgcctgcggc attctcaatg gcaatgctcg gcgcaatcga
    37741 atctctgctc tgcgccgtgg tgctggatgg tatgaccggg acgaagcaca aggcgaatag
    37801 cgaactggtt ggacaggggc tggggaatat catcgctccg ttctttggtg gtattaccgc
    37861 taccgctgcc atcgcgcgtt ctgccgctaa cgtccgtgcc ggggcaactt cccctatctc
    37921 ggcggtgatc cactctattc tggttattct tgccctgctg gtactggcac cgctgctctc
    37981 ctggctgccg ctttccgcta tggcagccct gctgttgatg gtggcgtgga acatgagtga
    38041 agcgcataaa gtggtcgact tgctgcgtca tgcaccgaaa gatgacatca ttgtcatgct
    38101 gctgtgcatg tcgctgaccg tgctgtttga tatggttatt gccatcagcg tggggatcgt
    38161 gctggcatcg ctgctgttta tgcgtcgtat cgcacgtatg actcgcctgg caccggtagt
    38221 cgtagatgtt ccagacgatg tcctggttct gcgcgttatt ggcccgctgt tttttgctgc
    38281 tgctgaaggc ttattcacgg acctggagtc acgtcttgaa ggcaaacgga ttgtgattct
    38341 gaagtgggat gccgttccgg tacttgatgc tggtggtctt gatgcgttcc agcgttttgt
    38401 gaagcgtctg cccgaaggat gtgaactgcg cgtgtgcaac gtggaattcc agccactgcg
    38461 cactatggct cgcgcaggca ttcaaccgat cccgggacgc ctcgcgttct tcccgaatcg
    38521 tcgcgcggcg atggcggatt tataagtacg agattgacca gtcagcgcaa actgactggt
    38581 cagcaaactg cattatttgt tagctatgac ggcgattatc gctacggcgg caacgtttgt
    38641 catagtggcg aacccaccag taacggtcgc acacctgttc atgcccgcca acacgggcac
    38701 ccgccagcca gagaatggca ccgacgaaaa tacttagcac tgcaccatgt gcgaaatact
    38761 ggggcatatt aaactgtggt aactggttga ggattgaata ccccacgccg accaccatta
    38821 ccaccagacc caaccccatg agcacgttac cgagtaacga agcgtttttg cgtttcatat
    38881 gtcacctccg gaactttctg ggttgtaaca gggaataccc ctcttcctta tgtgtaaagt
    38941 atagacaaca ccctgtgggt tatgtgcggg cgtgatcaca attacaaccc ttatttcaac
    39001 aaaactttac aaataaacgc ctgaacacct tgactttcta atagtccact caggcaatta
    39061 ttacgttatc tgcatttgat acgcagtttt ttgtcaagcg gggccgcaac cagtaaacta
    39121 cgcgccagtt atgtacacac tcaggacaaa aaaacgtgac gattaaattg attgtcggcc
    39181 tggcgaaccc cggtgctgaa tacgccgcaa cgcgacataa tgctggtgcc tggttcgttg
    39241 acttactggc agagcgtttg cgcgctccgc tgcgcgaaga ggctaaattc tttggttata
    39301 cttcgcgagt cacccttgga ggcgaagatg tccgcctgtt agtcccgact acatttatga
    39361 atctcagcgg caaagccgtt gcggcgatgg ccagtttttt ccgcattaat ccggacgaaa
    39421 ttctggtggc ccacgacgaa ctggatctgc ctcctggcgt cgccaaattt aagttgggcg
    39481 gtggccatgg tggtcacaat ggactgaaag acatcatcag taaattgggt aataacccta
    39541 actttcaccg tttacgcatc ggaatcggtc atccgggcga taaaaacaaa gttgttggtt
    39601 ttgtgttagg caaaccgcct gttagtgaac agaagttaat tgatgaagcc atcgacgaag
    39661 cggcgcgttg tactgaaatg tggtttacag atggcttgac caaagcaacg aaccgattgc
    39721 acgcctttaa agcgcaataa gtcgttgtct gcggcatttt tgccgagtgc cgtgtataat
    39781 aggcaaagtt atttccattt ctgcaatctg ttagcaataa caggttgatt attaagatat
    39841 taaggtgatt taaatcatgg gattcaaatg cggtatcgtc ggtttgccca acgtcgggaa
    39901 atctaccctg ttcaacgcgc tgaccaaagc cggtattgaa gcggccaact ttccattctg
    39961 caccattgag ccgaacacag gcgtcgtacc aatgcctgat cctcgcctgg atcaactggc
    40021 tgaaatcgta aaaccgcaac gtacgcttcc cacgaccatg gaatttgtcg atatcgccgg
    40081 tctggtaaaa ggcgcatcga aaggcgaagg tctgggtaac cagttcctga ccaacatccg
    40141 tgaaaccgaa gcgatcggtc acgttgttcg ctgctttgaa aatgacaaca tcattcacgt
    40201 ttccggcaaa gttaacccgg ctgacgatat tgaagttatc aacaccgaac tggcgctggc
    40261 agacctcgac acctgcgaac gtgcgattca tcgcgtacag aagaaagcca aaggtggcga
    40321 taaagacgcg aaagctgagc tggcggtcct ggaaaaatgc ctgccccagc tggaaaacgc
    40381 aggtatgctg cgcgcgctgg atttaagcgc tgaagagaaa gcggctattc gttacctgag
    40441 cttcctgacg ctgaaaccaa caatgtacat cgccaacgtc aacgaagacg gttttgaaaa
    40501 caacccatat cttgaccagg tgcgtgaaat cgcggcgaaa gaaggttctg ttgtggttcc
    40561 ggtttgtgct gctgttgaag cagacattgc cgaactggac gacgaagaac gtgacgagtt
    40621 tatgcaggag cttgggctgg aagagccggg tctgaaccgt gtgatccgtg ccggttataa
    40681 gctgctgaac ctgcaaactt acttcaccgc tggggtgaaa gaagtgcgtg catggaccat
    40741 tccggttgga gcaaccgcgc cgcaggcagc gggcaaaatc catactgatt ttgaaaaagg
    40801 ctttatccgt gcacaaacca tctcgtttga agatttcatc acttacaaag gtgaacaagg
    40861 cgcgaaagaa gcaggcaaaa tgcgtgcaga aggtaaagat tacatcgtga aagatggcga
    40921 tgtgatgaac ttccttttca acgtctaatt aatttattgt ctcatgaggt ttcgctaaat
    40981 ctcatgaaga gcgcaaaagt atataaaatc cacgcaattg cgtggatttt tcatttcacc
    41041 gagtcctaat cattcttgta acatcctaca cttttgtgct tattgcctac agtgaaccac
    41101 gagaataatt aaagacatca gtgttcagat gacggatatc tcattagcag tgatataacg
    41161 tcagtttttg ttccactaca catcagcccc cttcacagcc gatcaatatt gaccacgctc
    41221 caatatgctg gcgagaaaag gacttacctg aagggtaata tcccgcagat aaagtccccg
    41281 aataacgtga ttacgtgcga tcctggcaag atgaaaatga taaccagtag cacaataaca
    41341 acgatagacc agaaaaccca ttgaccaaaa atgaatatcg atgttttttg ctttttctta
    41401 ttagtaatgc aaaaatggcg catattgttt ttgttatgca gatacatcaa gcgaaaatcc
    41461 cataactaat taggattatt accaaaaata ttcac
//
LOCUS       DAZFBR010000027        40722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFBR010000027 DAZFBR010000000
VERSION     DAZFBR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.027"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            112..723
                     /gene="ruvA"
                     /locus_tag="ACHJZ2_001673"
     CDS             112..723
                     /gene="ruvA"
                     /locus_tag="ACHJZ2_001673"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO4567806.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            732..1742
                     /gene="ruvB"
                     /locus_tag="ACHJZ2_001674"
     CDS             732..1742
                     /gene="ruvB"
                     /locus_tag="ACHJZ2_001674"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO4567807.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1988..2773)
                     /gene="znuB"
                     /locus_tag="ACHJZ2_001675"
     CDS             complement(1988..2773)
                     /gene="znuB"
                     /locus_tag="ACHJZ2_001675"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO4567808.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2770..3525)
                     /gene="znuC"
                     /locus_tag="ACHJZ2_001676"
     CDS             complement(2770..3525)
                     /gene="znuC"
                     /locus_tag="ACHJZ2_001676"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO4567809.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3604..4536
                     /gene="znuA"
                     /locus_tag="ACHJZ2_001677"
     CDS             3604..4536
                     /gene="znuA"
                     /locus_tag="ACHJZ2_001677"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO4567810.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4552..5874
                     /gene="mepM"
                     /locus_tag="ACHJZ2_001678"
     CDS             4552..5874
                     /gene="mepM"
                     /locus_tag="ACHJZ2_001678"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO4567811.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5994..6965
                     /gene="lpxM"
                     /locus_tag="ACHJZ2_001679"
                     /gene_synonym="msbB"
     CDS             5994..6965
                     /gene="lpxM"
                     /locus_tag="ACHJZ2_001679"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO4567812.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7096..8538)
                     /gene="pyk"
                     /locus_tag="ACHJZ2_001680"
     CDS             complement(7096..8538)
                     /gene="pyk"
                     /locus_tag="ACHJZ2_001680"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO4567813.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8666..9535)
                     /gene="hexR"
                     /locus_tag="ACHJZ2_001681"
                     /gene_synonym="yebK"
     CDS             complement(8666..9535)
                     /gene="hexR"
                     /locus_tag="ACHJZ2_001681"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO4567814.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9873..11348
                     /gene="zwf"
                     /locus_tag="ACHJZ2_001682"
     CDS             9873..11348
                     /gene="zwf"
                     /locus_tag="ACHJZ2_001682"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO4567815.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11583..13394
                     /gene="edd"
                     /locus_tag="ACHJZ2_001683"
     CDS             11583..13394
                     /gene="edd"
                     /locus_tag="ACHJZ2_001683"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO4567816.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            13431..14072
                     /gene="kdgA"
                     /locus_tag="ACHJZ2_001684"
                     /gene_synonym="eda"
     CDS             13431..14072
                     /gene="kdgA"
                     /locus_tag="ACHJZ2_001684"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO4567817.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14128..15306)
                     /gene="purT"
                     /locus_tag="ACHJZ2_001685"
     CDS             complement(14128..15306)
                     /gene="purT"
                     /locus_tag="ACHJZ2_001685"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO4567818.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            15440..15730
                     /gene="yebG"
                     /locus_tag="ACHJZ2_001686"
     CDS             15440..15730
                     /gene="yebG"
                     /locus_tag="ACHJZ2_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO4567819.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASRAA"
     gene            15797..16153
                     /gene="yebF"
                     /locus_tag="ACHJZ2_001687"
     CDS             15797..16153
                     /gene="yebF"
                     /locus_tag="ACHJZ2_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO4567820.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            16480..17139
                     /gene="yebE"
                     /locus_tag="ACHJZ2_001688"
     CDS             16480..17139
                     /gene="yebE"
                     /locus_tag="ACHJZ2_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HFO4567821.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17348..19408
                     /gene="ptrB"
                     /locus_tag="ACHJZ2_001689"
     CDS             17348..19408
                     /gene="ptrB"
                     /locus_tag="ACHJZ2_001689"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO4567822.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(19405..20067)
                     /gene="exoX"
                     /locus_tag="ACHJZ2_001690"
     CDS             complement(19405..20067)
                     /gene="exoX"
                     /locus_tag="ACHJZ2_001690"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO4567823.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20091..20747)
                     /gene="yobB"
                     /locus_tag="ACHJZ2_001691"
     CDS             complement(20091..20747)
                     /gene="yobB"
                     /locus_tag="ACHJZ2_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO4567824.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(20849..21079)
                     /gene="holE"
                     /locus_tag="ACHJZ2_001692"
     CDS             complement(20849..21079)
                     /gene="holE"
                     /locus_tag="ACHJZ2_001692"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO4567825.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21218..21592
                     /gene="yobA"
                     /locus_tag="ACHJZ2_001693"
     CDS             21218..21592
                     /gene="yobA"
                     /locus_tag="ACHJZ2_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO4567826.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            21596..22468
                     /gene="copD"
                     /locus_tag="ACHJZ2_001694"
     CDS             21596..22468
                     /gene="copD"
                     /locus_tag="ACHJZ2_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO4567827.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            22481..22822
                     /gene="yebY"
                     /locus_tag="ACHJZ2_001695"
     CDS             22481..22822
                     /gene="yebY"
                     /locus_tag="ACHJZ2_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO4567828.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            23218..23874
                     /gene="pphA"
                     /locus_tag="ACHJZ2_001696"
     CDS             23218..23874
                     /gene="pphA"
                     /locus_tag="ACHJZ2_001696"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO4567829.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(23875..24066)
                     /gene="yebW"
                     /locus_tag="ACHJZ2_001697"
     CDS             complement(23875..24066)
                     /gene="yebW"
                     /locus_tag="ACHJZ2_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO4567830.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(24171..24407)
                     /gene="yebV"
                     /locus_tag="ACHJZ2_001698"
     CDS             complement(24171..24407)
                     /gene="yebV"
                     /locus_tag="ACHJZ2_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO4567831.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(24525..25964)
                     /gene="rsmF"
                     /locus_tag="ACHJZ2_001699"
     CDS             complement(24525..25964)
                     /gene="rsmF"
                     /locus_tag="ACHJZ2_001699"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO4567832.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(26045..28678)
                     /gene="yebT"
                     /locus_tag="ACHJZ2_001700"
     CDS             complement(26045..28678)
                     /gene="yebT"
                     /locus_tag="ACHJZ2_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO4567833.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(28647..29930)
                     /gene="yebS"
                     /locus_tag="ACHJZ2_001701"
     CDS             complement(28647..29930)
                     /gene="yebS"
                     /locus_tag="ACHJZ2_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO4567834.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            30060..30557
                     /gene="msrC"
                     /locus_tag="ACHJZ2_001702"
     CDS             30060..30557
                     /gene="msrC"
                     /locus_tag="ACHJZ2_001702"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO4567835.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            30654..31352
                     /gene="proQ"
                     /locus_tag="ACHJZ2_001703"
     CDS             30654..31352
                     /gene="proQ"
                     /locus_tag="ACHJZ2_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO4567836.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            31372..33420
                     /gene="prc"
                     /locus_tag="ACHJZ2_001704"
     CDS             31372..33420
                     /gene="prc"
                     /locus_tag="ACHJZ2_001704"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO4567837.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            33612..34493
                     /gene="htpX"
                     /locus_tag="ACHJZ2_001705"
     CDS             33612..34493
                     /gene="htpX"
                     /locus_tag="ACHJZ2_001705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO4567838.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(34539..35912)
                     /gene="yebQ"
                     /locus_tag="ACHJZ2_001706"
     CDS             complement(34539..35912)
                     /gene="yebQ"
                     /locus_tag="ACHJZ2_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4567839.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            36089..36880
                     /gene="kdgR"
                     /locus_tag="ACHJZ2_001707"
     CDS             36089..36880
                     /gene="kdgR"
                     /locus_tag="ACHJZ2_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO4567840.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(37024..37263)
                     /gene="yobH"
                     /locus_tag="ACHJZ2_001708"
     CDS             complement(37024..37263)
                     /gene="yobH"
                     /locus_tag="ACHJZ2_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO4567841.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            37423..37566
                     /gene="mgrB"
                     /locus_tag="ACHJZ2_001709"
     CDS             37423..37566
                     /gene="mgrB"
                     /locus_tag="ACHJZ2_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO4567842.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            37641..37928
                     /gene="yebO"
                     /locus_tag="ACHJZ2_001710"
     CDS             37641..37928
                     /gene="yebO"
                     /locus_tag="ACHJZ2_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO4567843.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
     gene            38597..38740
                     /gene="yobF"
                     /locus_tag="ACHJZ2_001711"
     CDS             38597..38740
                     /gene="yobF"
                     /locus_tag="ACHJZ2_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO4567844.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            38753..38962
                     /gene="cspE"
                     /locus_tag="ACHJZ2_001712"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             38753..38962
                     /gene="cspE"
                     /locus_tag="ACHJZ2_001712"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO4567845.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            39128..39937
                     /gene="rlmA"
                     /locus_tag="ACHJZ2_001713"
     CDS             39128..39937
                     /gene="rlmA"
                     /locus_tag="ACHJZ2_001713"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO4567846.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQKSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQILAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(39934..40500)
                     /gene="mntP"
                     /locus_tag="ACHJZ2_001714"
     CDS             complement(39934..40500)
                     /gene="mntP"
                     /locus_tag="ACHJZ2_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO4567847.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(40497..40619)
                     /locus_tag="ACHJZ2_001715"
     CDS             complement(40497..40619)
                     /locus_tag="ACHJZ2_001715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567848.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
BASE COUNT        10447 a         9989 c        10449 g         9837 t
ORIGIN      
        1 ttttcagttc atcgagacac ctcgcaagtt tttttcatcc ttcgctggat atctatccag
       61 cattttttta tcatacagca ttatctttga ttcattacgc aggagcgtca tgtgataggc
      121 agactcagag gcatcatcat tgaaaaacaa cccccgctgg tgttaattga agtgggcggc
      181 gtaggctatg aagtgcatat gccgatgacc tgtttttatg aactccctga agcgggtcag
      241 gaagcgatcg ttttcaccca ctttgtggtg cgtgaagacg cgcaactgct gtacggtttt
      301 aacaataaac aagagcgcac gttgttcaaa gagttgatca aaaccaacgg cgtcggcccg
      361 aagttggcgc tggcgatcct ctccggaatg tcagcgcagc agttcgttaa tgccgttgag
      421 cgtgaagaag tgggggcact ggtgaaactg ccgggtattg gcaaaaaaac cgccgaacgc
      481 ttgattgttg aaatgaaaga ccgatttaaa ggtttgcatg gcgatctctt tacgccagcc
      541 gccgacctgg tactcacgtc tcctgccagc ccggcgaccg acgatgctga acaagaagcg
      601 gttgccgcgc tggtggcgct gggctataaa ccacaagaag caagccgcat ggtgagcaaa
      661 atcgctcgcc ctgacgccag cagtgaaaca ttaattcgcg aagccctacg cgccgcgtta
      721 tgaggtaaag gatgattgaa gcagaccgtc tgatttctgc cggtaccact ttgccggaag
      781 atgtagcaga tcgcgccatt cgccctaaat tactggaaga gtatgttggt cagccgcagg
      841 ttcgttcaca gatggagatt ttcatcaaag cagcgaaact gcgcggcgat gccctcgatc
      901 atttgttgat ttttggtcct ccggggttgg gtaaaactac gcttgccaac attgtcgcca
      961 atgaaatggg cgttaattta cgcacgactt ctggtccggt gctggaaaag gcgggcgatt
     1021 tggctgcgat gctcactaac cttgaaccgc atgacgtgct gtttattgat gagatccacc
     1081 gtttatcgcc agttgttgaa gaagtgctgt acccggcaat ggaagactac caactggata
     1141 tcatgattgg tgaaggtccg gcggcacgct ccattaaaat tgatttgccg ccgtttaccc
     1201 tgattggtgc aaccacgcgc gcaggttcgc tgacatcacc gttgcgcgac cgttttggta
     1261 ttgtgcaacg tctggagttt tatcaggtgc cggatctgca atatatcgtc agtcgcagcg
     1321 cacgctttat ggggcttgag atgagtgatg acggcgcgct ggaagttgct cgtcgcgctc
     1381 gcggtacgcc gcgcattgcc aaccgtctgc tgcgtcgagt gcgtgatttc gccgaagtga
     1441 agcacgatgg caccatctcg gcagatatcg ctgctcaggc gctggatatg ttgaatgtcg
     1501 atgctgaagg tttcgattat atggaccgca aattgttgct ggcggtaatc gataagttct
     1561 ttggtgggcc ggtaggtctg gataaccttg cggcagccat tggcgaagaa cgtgaaacca
     1621 ttgaggatgt gctggaacct tatttgattc agcaaggctt tttgcagcgt acaccgcgtg
     1681 ggcgtatggc gacgacgcgg gcgtggaatc actttggcat aacgccgcca gaaatgccgt
     1741 aagtcggatt ggtcgctcgc accgcatccg acattggcag tgtgcctgat gcgacgctat
     1801 tcgcgtctta tcaggcatat accagagata gcgaaccgta ggtcggaaaa ggcgctcgcg
     1861 ccgcatccga cattggcagt gtgcctgatg cgacgctgtt cgcgtcttac caggcctacc
     1921 agagatagcg aaccgtaggt cggataaggc gctcgcgccg catccgacaa atgtgttcag
     1981 cgatagatta gctggcctgc tttttcatca tactgataat aaataacagt gccgcacata
     2041 gcaccaccga cggacccgcc ggcgtatcgt aaaacgcgga aaaggttaaa ccgtcagtta
     2101 ctgccaccat ccccaccaaa acagcgacac cagccatctg ttccggcgta cgggcaaagc
     2161 gacgcgcagt agctgcagga ataatcagca acgaagtaat aatcaacgca ccgacaaatt
     2221 tcatcgctac accaatcgtc aatgccgtca ccagcatcaa caacaatttc actcgctgta
     2281 atttcacacc atcaacaaac gccagatccg ggctaatcgt catcgacagc aaattgcgcc
     2341 attgccagaa caaaatagcc accacgatga ccacgccaat tgcaatagag atgagatctt
     2401 ctggcgtcac tgccagtaaa tcaccgaaca ggtaagccat caaatcaaca cgaatgttag
     2461 acatcagact aacgaccacc aggcccagcg acagggcact gtgcgccata atccctaata
     2521 acgtgtcgat cgccagctgt ggacgcttct ccagccatac cagaccgccc gccagcagca
     2581 gcgtaacggc aatcaccgca tagaatggat tcacgtccag caacaaacca aacgcgacac
     2641 caagtaatga ggcatgagcc agcgtatcac cgaaataaga catacgacgc cagactacaa
     2701 acgaacccag cggacccgcg gcacaggcga gcatgatccc ggctaaccaa ccgggaaata
     2761 ataattcaat catgagcgat catttccccg acgcaaaaca attcgtccct gtaaatcgtg
     2821 acgatgatta tgatgatggc gatagatacc cagttgttca gcaccacgag gaccaaacat
     2881 tgaaataaac tccggatgca gggaaacaac ttccggtgtg ccggaacaac aaatgtgatg
     2941 attcaggcac agcacttcat cggtttttgc catcaccaga tgaagatcgt gagaaaccat
     3001 taaaacgcca caatccagtt cacgacgcag ttggtcaata aggtcatata acgccacctg
     3061 gccatttaca tccacgcctt gagtgggttc atccagcact aataattgcg ggcgatttaa
     3121 caatgctcgc gctaacagta cacgctgcgt ttcgccacca gagagctttt gcatcggtgc
     3181 gttaatcagg tgcccggcct ggacacgttt cagtgcaggc aaaatatctt ctttatgtgt
     3241 accagggcgt aagcgtaaaa aacggtttac ggtcagtggc aacgtggtgt cgagatacag
     3301 cttctgcggt acatagccga tgcgcagttt tccgttgcgc ttgataaccc cttcatcggg
     3361 tgttaccagc ccgagcacta cccgtaccag tgtcgactta cctgcgccat ttggcccaag
     3421 taaagtcaaa atttttccag gtttaagttc cagcgacaca tcagagagga cgcggcgttg
     3481 gccaaaagaa accgagacat tttccaggga aaccagactt gtcatgttaa ttttagtctt
     3541 gcagtagtca tgaaatgtta taatatcaca cttctcatat tcattacgat gattggtcgc
     3601 attatgttac ataaaaaaac gcttcttttc gcagcattat tcgccgctct ctggggcggt
     3661 gcaacacagg ccgcagatgc cgccgttgtc gcttcgctta aacccgttgg gttcatcgct
     3721 tctgccattg ctgatggggt aacagaaacg gaggttttac ttcctgacgg cgcttcagaa
     3781 catgattatt cactgcgtcc atcggatgta aaacgcttac agaacgcgga cttagtcgtt
     3841 tgggttggcc cggagatgga agcgtttatg caaaaaccgg taagcaaatt accagaagcg
     3901 aagcaggtaa cgattgcgca gcttgaagat gtgaaaccgc tactgatgaa aagtattaac
     3961 ggcgatgatg atgatcacga ccacgcggaa aaaagtgacg aagatcacca tcacggcgat
     4021 ttcaacatgc atctttggct ttccccagag atagcgcggg ctacagcggt tgcaatccat
     4081 ggaaaattag tggaacttat gccgcaaagt cgagccaaac ttgacgccaa cctgaaggat
     4141 tttgaggcac aattagcctc aaccgaaacg caggttggta acgagctcgc gccgctcaag
     4201 gggaaaggtt atttcgtttt tcacgatgct tacggctatt tcgaaaaaca gttcggactg
     4261 acaccgcttg gtcattttac cgttaaccct gagattcaac ctggcgcgca gcgtttacat
     4321 gaaataagaa cacagttggt tgagcaaaaa gcaacctgcg tttttgctga gccacagttc
     4381 aggccagcgg tcgttgagag cgtcgcacga gggacatccg ttcgtatggg aacgttggat
     4441 cccttgggga cgaatatcaa actgggtaaa acaagctatt cagaattcct gagtcaatta
     4501 gccaaccagt atgcgagctg cctgaaagga gattaatgag gaagtgatta cgtgcaacag
     4561 atagcccgct ctgtcgccct ggcgtttaat aatttaccgc gaccacaccg cgttatgttg
     4621 gggtcgctca ccgttcttac tctggccgtc gctgtctggc ggccctatgt ttatcaccgc
     4681 gatgccacgc caattgtcaa aaccattgag ctggaacaga acgaaattcg ttcgctctta
     4741 cctgaagcca gtgagccgat tgatcaagct gcacaagaag atgaagccat tccccaggac
     4801 gaactggatg acaaaatcgc cggtgaagcg ggcgtgcatg aatatgttgt ttccactggc
     4861 gatacgctaa gcagcattct caatcagtat ggtattgata tgggtgatat cacccaactg
     4921 gctgcggccg acaaagaatt gcgtaacctg aaaatcggtc aacaactctc ctggacatta
     4981 accgcggacg gcgaactgca gcgcctcacc tgggaagtgt ctcgtcgtga aacccgaacc
     5041 tatgaccgta ctgccgctaa cggttttaaa atgaccagcg aaatgcagca aggagagtgg
     5101 gttaacaatc tgctgaaagg taccgtcggg gggagctttg ttgccagcgc cagaaacgcc
     5161 ggtttgacca gcgccgaagt gagcgcagtg attaaagcca tgcagtggca aatggatttc
     5221 cgcaaactga aaaaaggcga tgaatttgcg gtattaatgt ctcgagaaat gcttgatggt
     5281 aaacgtgagc aaagccagct gctgggcgta cgtttgcgtt cagaaggtaa agattattac
     5341 gctatccgcg ctgaagatgg caaattctac gatcgtaacg gtactggtct ggcgaaagga
     5401 ttcttgcgat tcccgacggc gaaacagttc cgtatctcgt ctaactttaa cccgcgtcgt
     5461 actaatccgg tgaccggtcg cgttgcacca cacagaggtg ttgatttcgc catgccgcag
     5521 ggtacgccag tgctttcagt gggtgacggt gaagtggtgg ttgccaaacg cagtggcgca
     5581 gcaggttatt atgtggctat tcgtcatggt cgcagctaca ccacgcgtta tatgcacttg
     5641 cgcaagattc tggtgaaacc ggggcagaag gtgaaacgtg gcgaccgtat cgcgctttcc
     5701 ggtaataccg gacgttcaac cgggccgcat ctgcactatg aagtatggat aaaccagcag
     5761 gccgtaaacc cgctgacggc aaaactgccg cgtaccgaag ggctgaccgg ctccgatcgt
     5821 cgcgaattcc tggcgcaggc caaagagatt gtgccgcagc tacggtttga ttaattaaca
     5881 tccattcgca gccggtacgc agtcagtacc ggcttttttt atttggtgcg gggcaagttg
     5941 cgccgctaca ctatcaccag attgattttt gccttatccg aaactggaaa agcatggaaa
     6001 cgaaaaaaaa taatagcgaa tacattcctg agtttgataa atcctttcgc cacccgcgct
     6061 actggggagc atggctgggc gtagcagcga tggcgggtat cgctttaacg ccgccaaagt
     6121 tccgtgatcc cattctggca cggctgggac gtattgccgg acgactggga aaaagctcac
     6181 gccgtcgtgc gttaatcaat ctgtcgctct gctttccaga acgtagtgaa gctgaacgcg
     6241 aagcgattgt tgatgagatg tttgccaccg cgccgcaagc gatggcaatg atggctgagt
     6301 tggcaatacg cgggccggag aaaattcagc cgcgcgttga ctggcaaggg ctggagatca
     6361 tcgaagagat gcggcgtaat aacgagaaag ttatctttct agtgccgcac ggttgggccg
     6421 tcgatattcc tgccatgctg atggcctcgc aagggcagaa aatggcagcg atgttccata
     6481 atcagggcaa cccggttttt gattatgtct ggaacacggt gcgtcgtcgc tttggcggtc
     6541 gtctgcatgc gagaaatgac ggtattaaac cattcatcca gtcggtacgt caggggtact
     6601 ggggatatta tttacccgat caggatcatg gcccagagca cagcgaattt gtggatttct
     6661 ttgccaccta taaagcgacg ttgcccgcga ttggtcgttt gatgaaagtg tgccgtgcgc
     6721 gcgttgtacc gctgtttccg atttatgatg gcaagacgca tcgtctgacg attcaggtgc
     6781 gcccaccgat ggatgatctg ttagaggcgg atgatcatac gattgcgcgg cggatgaatg
     6841 aagaagtcga gatttttgtt ggtccgcgac cagaacaata cacctggata ctaaaattgc
     6901 tgaaaactcg caaaccgggc gaaatccagc cgtataagcg caaagatctt tatcccatca
     6961 aataaaaaag cctctcgcga ggagaggcct tcgcctgatg ataagttcaa gtttgcttca
     7021 gaatattcga aatctgttga actatcattg aactgtaggc cggatgtggc gttttcgccg
     7081 catccggcaa cgtacttact ctaccgttaa aatacgcgtg gtattagtag aacccacggt
     7141 actcatcacg tcgccctggg tgacaatcac caggtcacca gacatcaagt aacctttatc
     7201 gcgcagcaga ttaaccgctt cgctggcagc tgctacgccg tcattagcgc tatcaaagtg
     7261 caccggcgta acgccacgat agagagcagt caggttcagc gtacgttcat ggcgcgacat
     7321 ggcgaaaatt ggtagaccag agctgatacg ggaggtcatc agcgcggtac gacccgattc
     7381 ggtcatggta atgatcgccg taacgccttt caggtggtta gctgcgtaca ttgctgacat
     7441 ggcaatagct tcttccacat tgtcgaactg aacgtccaga cggtgtttag aaacgttgat
     7501 gctcgggatt ttttccgcac ccaggcaaac gcgcgccatg gctgcaacag tttctgacgg
     7561 atactgccca gcggcagttt ctgcagacag catcacagcg tcagtaccat ccagaacggc
     7621 gtttgctacg tccatgactt ctgcacgcgt cggcatcggg ttagtaatca ttgactccat
     7681 catctgggtc gccgtgatta ccgctcggtt tagctgacgc gcacgacgga tcaacgcttt
     7741 ctgaatgccg accagttccg ggtcgccaat ttccacaccg aggtcgccac gtgcaaccat
     7801 taccacgtca gaggcgagga tgatgtcatc cattgcatcc tggctgcaaa cggcttccgc
     7861 acgttcaacc ttggcaacaa ttttcgcatc acatcctgca tcgcgtgcca ggcgacgggc
     7921 atagttcaga tcttcgccac agcgtgggaa ggagacagcc aggtaatcta cgccaatcaa
     7981 cgccgcagtc ttaatgtctg ctttgtcttt ttcggtcagc gcttcagccg acaaaccgcc
     8041 gccaagtttg ttgatacctt tattgttgga gaggggacca ccgacggtga cttcggtgaa
     8101 cactttcatg ccctgaactt ccagtacttt taactggacg cgaccatcgt ccagcagcag
     8161 gatgtcacca ggcacgacgt cagcaggcag gcctttgtag ttgataccga ctttttcttt
     8221 gtcgccttca cctttaccca ggttggcgtc gagcaggaat ttatccccaa tattgaggaa
     8281 aactttgcct tctttaaagg tggatacacg gattttgggc ccctggaggt cacccagaat
     8341 agccacatga cgccccagtt ttgcggcaat ctcacgaact ttatccgcgc gcattttgtg
     8401 atcttcaggc gagccgtgag aaaagttcat acgtacaacg ttggcacccg ccgcgataac
     8461 tttttcaaga ttattatcgc gatctgttgc tgggcctaac gtggtaacga tttttgttct
     8521 gcgaagcctt ctggacatgt aatactccgt tgactgaaac aaccaggtgt tgcttgaaca
     8581 tgaaatccga atgaaataac gccgcgatgg cgataaaata atctcattcg gatgtcgtat
     8641 aagattagaa cagtgacagt cgcttttagc gatcgtcact taaattaagt aactgcttat
     8701 caaaacgcga ttccttcagc gcttccttga cccgcttcaa gttatctctg aattttgcac
     8761 cgcgtcgcaa agtaaatcct gtcgccagca catctatcac ggtcagctgt gcaagtcgag
     8821 aaaccatggg catataaatg tcagtatctt ccggtacgtc gagggtaatt gccagcgttg
     8881 cttcccgggc gagcggggta cccgcagagg tgagggcaat caccatagcg tcgttttcgc
     8941 gtgccagctg cgccagctcg accagatttt ttgttcttcc ggtgtgagaa atcagcacca
     9001 ccacgtctcc gtcgctacaa ttcatacaac tcatgcgttg cagcacaata tcatcggagt
     9061 acaccaccgg aacattaaaa cgaaagaact tattcatcgc atcgtgggca acggcggctg
     9121 aagagcctaa tccgaaaaag gcgatttttt ttgcctgagt gagcaagtcg acggcgcggt
     9181 tgatggcaga tttatccagt gaatgacgga catgatcgag cgttgccatt gcggactcaa
     9241 atattttccc tgtgtatgat tcaacgctgt catcttcatt gacgttgcga ttaacatagg
     9301 gagtgccatt cgccagactc tgtgccagat gaagtttaaa atcaggaaaa ccgcgcgtgt
     9361 ccatgctgcg acagaaacga ttcaccgtcg gttcgctaac attggcttcc agtgccagag
     9421 cagcaatact cgaatggatc gcgttatcgg gcgaagccag aatgacctcg gcaactttgc
     9481 gctctgattt gctcaaatgt tccagctgag actggatttt ttccagcata ttcatgatgt
     9541 aaagagactc acgggtaatg acgatttccg cactgaaaga aatcgaaatg cagttttgtc
     9601 agatattacg cctgtgtgct gtgttaatga caaaagcaga taaaaaagtt gttatttttt
     9661 ttcataacat gatcagtgtc agatttttac ccaatggaaa acgatgattt ttttatcagt
     9721 tttgccgcac tttgcgcgct tttaccgtaa tcgcacgggt ggataagcgt ttacagtttt
     9781 cgcaagctcg taaaagcagt acagtgcacc gtaagaaaat tacaagtata ccctggctta
     9841 agtaccgggt tagttaactt aaggagaatg acatggcggt aacgcaaaca gcccaggtct
     9901 gtgacttggt cattttcggc gcgaaaggcg accttgcgcg tcgtaaattg ctgccttccc
     9961 tgtatcaact ggaaaaagcc ggtcagctca acccggacac ccggattatc ggcgtagggc
    10021 gtgctgactg ggataaagcg gcatatacca aagttgtccg cgaggcgctc gaaactttca
    10081 tgaaagaaac cattgatgaa ggtttatggg acaccctgag cgcacgtctg gatttttgta
    10141 atctcgatgt caatgacact gctgcattca gccgtctcgg cgcgatgctg gatcaaaaaa
    10201 atcgtatcac cattaactac tttgccatgc cgcccagcac ttttggcgca atttgcaaag
    10261 ggcttggcga ggcaaaactg aatgctaaac cggcacgcgt agtcatggag aaaccgctgg
    10321 ggacgtcgct ggcgacctcg caggaaatca atgatcaggt tggcgaatac ttcgaggagt
    10381 gccaggttta ccgtatcgac cactatcttg gtaaagaaac ggtgctgaac ctgttggcgc
    10441 tgcgttttgc taactccctg tttgtgaata actgggacaa tcgcaccatt gatcatgttg
    10501 agattaccgt ggcagaagaa gtggggatcg aagggcgctg gggctatttt gataaagccg
    10561 gtcagatgcg cgacatgatc cagaaccacc tgctgcaaat tctttgcatg attgcgatgt
    10621 ctccgccgtc tgacctgagc gcagacagca tccgcgatga aaaagtgaaa gtactgaagt
    10681 ctctgcgccg catcgaccgc tccaacgtac gcgaaaaaac cgtacgcggg caatatactg
    10741 cgggcttcgc ccagggcaaa aaagtgccgg gatatctgga agaagagggc gcgaacaaga
    10801 gcagcaatac agaaaccttc gtggcgatcc gcgtcgacat tgataactgg cgctgggccg
    10861 gtgtgccatt ctacctgcgt actggtaaac gtctgccgac caaatgttct gaagtcgtgg
    10921 tctatttcaa aacacctgaa ctgaatctgt ttaaagagtc gtggcaggat ctgccgcaga
    10981 ataaactgac tatccgtctg caacctgatg aaggcgtgga tatccaggta ctgaataaag
    11041 ttcctggcct tgaccacaaa cataacctgc aaatcaccaa gctggatctg agctattcag
    11101 aaacctttaa tcagacgcat ctggcggatg cctatgaacg tttgctgctg gaaaccatgc
    11161 gtggtattca ggcactgttt gtacgtcgcg atgaagtgga agaagcctgg aaatgggtag
    11221 actccattac tgaggcgtgg gcgatggaca atgatgcgcc gaaaccgtat caggccggaa
    11281 cctggggacc cgttgcctcg gtggcgatga ttacccgtga tggtcgttcc tggaatgagt
    11341 ttgagtaata tctgcgctta tcctttatgg ttattttacc ggtaacatga tcttgcacag
    11401 attgtagaac aatttttaca ctttcaggcc tcgtgcggat tcacccacga ggcttttttt
    11461 attacactga ctgaaacgtt tttgccctat gagctccggt tacaggcgtt tcactcataa
    11521 atcctctgaa tgaaacgcgt tgtgaatcat cctgctctga caactcaatt tcaggagcct
    11581 ttatgaatcc acaattgtta cgcgtaacaa atcgaatcat tgaacgttcg cgcgagactc
    11641 gctctgctta tctcgcccgg atagaacaag cgaaaacgtc gaccgttcat cgttcgcagt
    11701 tggcatgcgg taacctggca cacggtttcg ctgcctgcca gccagaagac aaagcctctt
    11761 tgaaaagcat gttgcgtaac aatatcgcca tcatcacctc ctataacgac atgctctccg
    11821 ctcaccagcc ttatgaacac tatccagaaa tcattcgtaa agccctgcat gaagcgaatg
    11881 cggttggtca ggttgcgggc ggtgttccgg cgatgtgtga tggtgtcacc caggggcagg
    11941 atggaatgga attgtcgctg ctaagccgcg aagtgatagc gatgtctgcg gctgtcgggc
    12001 tgtcccataa catgtttgat ggtgctctgt tcctcggggt gtgcgacaag attgtcccgg
    12061 gtctgacgat ggcagccctg tcgtttggtc atttgcctgc ggtgtttgtg ccgtctggac
    12121 cgatggcaag cggtttgcca aataaagaaa aagtgcgtat tcgccagctt tatgccgaag
    12181 gtaaagtgga ccgcatggcc ttactggagt cagaagccgc gtcttaccat gcgccgggaa
    12241 catgtacttt ctacggtact gccaacacca accagatggt ggtggagttt atggggatgc
    12301 agttgccagg ctcttctttt gttcatccgg attctccgct gcgcgatgct ttgaccgctg
    12361 ccgctgcgcg tcaggttaca cgcatgaccg gtaatggtaa tgaatggatg ccgatcggta
    12421 agatgatcga tgagaaagtg gtggtgaacg gtatcgttgc actgctggcg accggtggtt
    12481 ccactaacca caccatgcac ctggtggcga tggcgcgcgc ggccggtatt cagattaact
    12541 gggatgactt ctctgacctt tctgatgttg taccgctgat ggcacgtctc tacccgaacg
    12601 gtccggccga tattaaccac ttccaggcgg caggtggcgt accggttctg gtgcgtgaac
    12661 tgctcaaagc aggcctgctg catgaagatg tcaatacggt ggcaggcttt ggtctgtctc
    12721 gttataccct tgaaccatgg ctgaataatg gtgaactgga ctggcgggaa ggggcggaaa
    12781 aatcactcga cagcaatgtg atcgcttcct tcgaacaacc tttctctcat catggtggga
    12841 caaaagtgtt aagcggtaac ctgggccgtg cggttatgaa aacctctgcc gtgccggttg
    12901 agaaccaggt gattgaagcg ccagcggttg tttttgaaag ccagcatgac gttatgccgg
    12961 cctttgaagc gggtttgctg gaccgcgatt gtgtcgttgt tgtccgtcat caggggccaa
    13021 aagcgaacgg aatgccagaa ttacataaac tcatgccgcc acttggtgta ttattggacc
    13081 ggtgtttcaa aattgcgtta gttaccgatg gacgactctc cggcgcttca ggtaaagtgc
    13141 cgtcagctat ccacgtaaca ccagaagctt acgatggcgg gctgctggca aaagtgcgcg
    13201 acggggacat cattcgtgtg aatggacaga caggcgaact gacgctgctg gtagacgaag
    13261 cggaattggc tgctcgcgaa ccacacattc ctgacctgag cgcgtcacgc gtgggaacag
    13321 gacgtgaatt attcagcgcc ttgcgtgaaa aactgtccgg tgccgaacag ggcgcaacct
    13381 gtatcacttt ttaagacgac aaatttgtaa tcaggcgaga gaaaactctg atgaaaaact
    13441 ggaaaacaag tgcagaatca atcctgacca ccggcccggt tgtaccggtt atcgtggtaa
    13501 aaaaactgga acacgcggtg ccgatggcaa aagcgttggt tgctggtggg gtgcgcgttc
    13561 tggaagtgac tctgcgtacc gagtgtgcag ttgacgctat ccgtgctatc gccaaagaag
    13621 tgcctgaagc gattgtgggt gccggtacgg tgctgaatcc acagcagctg gcagaagtca
    13681 ctgaagcggg tgcacagttc gcaattagcc cgggtctgac cgagccgctg ctgaaagctg
    13741 ctaccgaagg gactattccg ctgattccgg ggatcagcac tgtttccgaa ctgatgctgg
    13801 gtatggacta cggtttgaaa gagttcaaat tcttcccggc tgaagctaac ggcggcgtga
    13861 aagccctgca ggcgatcgcg ggtccgttct cccaggtccg tttctgcccg acgggtggta
    13921 tttctccggc taactaccgt gactacctgg cgctgaaaag cgtgctgtgc atcggtggtt
    13981 cctggctggt tccggcagat gcgctggaag cgggcgatta cgaccgcatt actaagctgg
    14041 cgcgtgaagc tgtagaaggc gctaagctgt aaaagtcaaa atgcccgatc gaggatcggg
    14101 catttttgta gcgtttattt gccgggttta accctgtact tttacctgtc cggcggcgtg
    14161 cttcgcgcgt tcaatggcgt caacaacact ctctgcagta gccagtgcca cccccagacg
    14221 acggctgcca tcaatttccg gcttaccaaa taaacgaatc tgcaaatctg cgcctacggc
    14281 attctgcaca ttatcaaacg tgacattctg actggtcagt tgtggcagaa taacggcaga
    14341 agctgcagga ccatactgac ggatcccgcc aaccggaagt ccgaggaagg cacgtacatg
    14401 cagggcaaac tctgagagat cttgagaaat taacgtcacc atcccggtat catgtggacg
    14461 aggggagacc tcactgaaaa tcacctcatc accacagaca aacagctcga caccaaacag
    14521 accataaccg cccagtgcca gcaccacttt acgggcaatc tcctgcgcac gttcaagggc
    14581 aagcgggctc atttgctgtg gttgccagga ttcacagtag tcgccatctt cctggcgatg
    14641 acctactggt gcacagaaat ggacgccatc caccgcgctg acggttagca gggtaatttc
    14701 gaagtcaaac ttaacgacgc cttcaacaat tacgcggccc gctccggcgc gaccgccttg
    14761 ctgagcgtat tcccatgcct gagcaagttg ctctgcagag cgaataaacg tctgcccctt
    14821 gccggaagag ctcatcaccg gttttacaat gcagggatag ccaatggcgg caaccgcctc
    14881 gcggaaaagg ctttcgctat cggcaaaacg ataagtggaa gtgggcagct gcagctcttc
    14941 tgccgccagg cgacggatac cctcgcgatt catcgttaat ttcgttgcgc gagcgcaggg
    15001 gacaacattc agtccttcct cttcaagttg gatcagcata tcggtggcaa tagcttcgat
    15061 ctccggcacg atataatgtg gtttttccag ttcaaccaca cggcgtaatg catcaccatc
    15121 aagcatatta atgacatggg agcgatgcgc gacatgcatg gctggtgcgt cggcatagcg
    15181 atcgacggca atcacctcta cgccgagacg ctgacactcg attgccactt ctttacccag
    15241 ttcaccggag cctaataaca tcacgcgagt tgctgccgga cgcagcgcag tgcctaataa
    15301 cgtcataacg aatatcccct gattaattcc gcgcgcagta taaacgaaaa cgtttgcgtg
    15361 tgtctttatt gctggtgttg atctctcaac cgaaaagaaa tatactgtat agaatcacag
    15421 ttattatgag aggctgcaaa tggcggttga agttaaatac gtagtcattc gtgagggcga
    15481 agagaaaatg tcgtttacca gcaaaaagga agccgatgct tatgacaaaa tgcttgatac
    15541 ggcggatctg cttgacacct ggctgacaaa ttctccagtg caaatggaag acgagcaacg
    15601 tgaagccctt tcgctatggc tggcagaaca aaaagatgtg ctgagcacca ttctgaaaac
    15661 cggcaaatta ccgtccccac aggtcgtcgg tgctgaatcc gaagaggaag atgcctcacg
    15721 cgcggcctga agcaacttgc gctcctgaca atttgtacca tgctttctcc tgaatcattg
    15781 tgctatggag aaaaacatga aaaaaagagg ggcgttttta gggctgttgt tggtttctgc
    15841 ctgcgcatca gttttcgctg ccaataatga aaccagcaag tcggtcactt tcccaaagtg
    15901 tgaaggtctg gatgctgccg gaattgccgc gagcgtaaaa cgtgattatc aacaaaatcg
    15961 cgtggcgcgc tgggcagatg atcaaaaaat tgtcggtcag gccgatcccg tggcatgggt
    16021 cagtttgcag gacattcagg gtaaagatga taaatggtca gtaccgctaa ccgtacgtgg
    16081 taaaagtgcc gatattcatt accaggtcag cgtggactgc aaagcgggaa tggcggaata
    16141 tcagcggcgt taatgggttc tgataattcg attgaccgtt ggcggatgcg gcgcgagtgc
    16201 cttgtccgcc ccagaacgca ccacaatcgt aggcctgaga agacgcaaca gcgtcgcatc
    16261 gggcaatcag catcaaacaa tgccactcaa aacgacaaac tactttcacc taaaataccc
    16321 ttatcacctg atatacagta aataccttac ctggcgcgaa tttctttgaa taacggccta
    16381 ccgccccgct tatttgcgca tcttttgtct cgatatacat tttctgacat cctcatcctc
    16441 tatgctacag gcactaaaaa tggttattga ggagcaaata tggctaactg gttaaatcaa
    16501 ctgcaatccc tgcttgggca aagcagttct tctacctcct cgtctgcgga tcagggattg
    16561 ggcaaacttt tagtgccggg cgcattaggc ggactggctg gactgctggt cgcaaataaa
    16621 tcagcacgta aacttcttac gaaatatggc accaacgcgt tgctgattgg cggcggagcg
    16681 gtagcgggta cggtgctgtg gaataaatac aaagataaaa ttcgcgcggc gcatcaggac
    16741 gaaccgcagt ttggctcgca aagtacgccg ctggatgagc gtacggaacg tttgatcctt
    16801 gcgctagtct ttgccgctaa aagtgatggt catattgatg ccaaagaacg tgcggcaatc
    16861 gaccagcaat tgcgtgaagc cggcgtggaa gagcaggggc gggtactcat tgagcaggca
    16921 atcgaacaac cgctggatcc acaacgcctg gctaccgggg tccgcaatga agaagaggcg
    16981 ctggaaatct atttcctgag ctgcgcggct attgatattg accattttat ggaacgcagt
    17041 tacctgaatg cactgggtga cgcgctgaaa atcccccagg atgttcgtga aggcattgaa
    17101 cgcgatctcg aacagcaaaa acgcactttg gcggaataat tccgctctct ggatagcctg
    17161 aagttgacta tttcgggcta tccagaacag cacagccatt tgaacaggct gattttctct
    17221 gtctgcataa cattcgatat tcgcgccatt acctgtcatc atctaagcaa tgactgccct
    17281 gtttcgcttg catccccggt gagttttgcc acccttataa gatgtttcaa ccagaaagaa
    17341 caataacatg ctaccaaaag ccgcccgcat tccccacgcc atgacgcttc atggcgatac
    17401 gcgcatcgat aattactact ggctgcggga cgatacgcgt tctcagccgg aagtcctgga
    17461 ctacctgcaa caagaaaata gttacggtca tcgggtgatg gcctcacaac aagccttgca
    17521 ggatcgcatc ttaaaggaaa tcatcgaccg cattccgcaa cgagaagttt ctgcgcccta
    17581 catcaaaaat ggctaccgct accggcatat ttatgaacca ggctgtgaat atgctatcta
    17641 ccagcgtcaa tcggcgttca gtgaagagtg ggacgagtgg gaaatattgc tcgatgccaa
    17701 caagcgcgcg gctcatagtg agttttattc gatgggcgga atggcgatta cgcccgataa
    17761 caccattatg gcgctggcag aagattttct ttcccgacgc cagtacggca ttcgttttcg
    17821 taatctggaa acaggtaact ggtacccgga actgctggat aacgttgaac ccagctttgt
    17881 ctgggcaaat gactcctgga ctttctacta tgttcgcaag catccggtga cgctgctgcc
    17941 ttatcaggtc tggcgtcacg ccatcggtac gccagcatcg caagataaac tgatttacga
    18001 agaaaaagac gatacctatt acgtcagcct gcataaaacg acctcaaagc actatgtagt
    18061 cattcatttg gccagcgcca ccaccagtga agttcgcctg ctggacgcgg aaatggcaga
    18121 tgccgagccg tttgtttttc tgccgcgccg caaagatcac gaatacagcc ttgatcacta
    18181 ccagcatcgt ttttatctgc gttccaaccg ccacggcaaa aactttggct tataccgtac
    18241 ccgtatgcgt gatgagcaac agtgggaaga gttaattccg ccacgcgaaa acatcatgct
    18301 ggaagggttt acgctgttta ccgactggct ggtggttgaa gagcgtcagc gcgggttaac
    18361 cagtttgcgc caaattaacc gcaagacccg ggaagtcatt ggtattgcct ttgatgatcc
    18421 agcctatgtg acctggattg cctacaatcc agaacctgaa accgcgcgat tgcgttatgg
    18481 ttattcttcc atgaccacac cagacacttt gtttgaactg gatatggata ccggtgagcg
    18541 tcgtgtatta aaacaaacag aagttcctgg ttttgatgcg gcgaattacc gcagtgaaca
    18601 cctgtggata gtcgcccgtg atggtgtcga agttccggtt tcgttggtct accatcgcaa
    18661 tcattttcgc aaaggacaca acccgctgct ggtgtatggc tacggttctt acggcgcaag
    18721 tattgatgcc gatttcagtt ttagccgctt gagtttgtta gatcgtggct ttgtctacgc
    18781 cattgtccat gttcgcggcg gcggtgagct ggggcaacaa tggtacgaag acggtaaatt
    18841 tctgaagaag aaaaatacgt ttaatgatta tcttgatgcc tgcgatgcat tgttaaaact
    18901 gggctatggc tctccttcgc tttgttatgc gatgggcggg agtgcggggg gcatgttgat
    18961 gggcgttgca attaatcaac gcccggaatt attccacggc gttatcgccc aggtaccgtt
    19021 tgttgatgtt gtaacaacga tgcttgatga atcaattcct cttaccactg gtgagtttga
    19081 agagtggggg aatccgcagg atctgcaata ttacgagtat atgaaaagct acagcccgta
    19141 tgacaacgtc accgcacagg cttatccaca tttactggta acgaccggtt tacacgattc
    19201 tcaggtgcaa tattgggaac cggcaaaatg ggtcgctaag ttgcgcgagc tgaaaaccga
    19261 taaccatctt ttattgctct gtaccgacat ggactcaggc catggcggta aatctggtcg
    19321 ctttaaatcg tacgaaggcg tagcgatgga atatgctttt ctggtcgcgc tggcgcaggg
    19381 aacattaccc gctaggcctg cggattaggt attttccaga taatgtttca gtgttaaacg
    19441 cagctccggg ctcatgctgt ccaggttatt aaacaaccag cgcagatagc ccggatcgcg
    19501 ttcggcaacg tcggaaaccg ctttgccacg gtatttgcca aaggtgaagg tcgtcatcaa
    19561 cgacggacgt ccggttatat cggccatctg ttctgccgtc cagccggagg tgttcataat
    19621 atcgataagc aacgcggcgg tgatataaca gtcatacaac gcgcggtgat gatgcaggcc
    19681 cggcggcgtc tgtacattga gcttccgtgt tttatataac gccatattgc tgtacttgat
    19741 ccctggccac aaacgacggg ccagtttcat agtgcaaatc cactcaccgg gcatctcagg
    19801 cagtacgcgg cggtcaaagc tggcgttatg cgcgacatac cattcactac cgtaatagtg
    19861 tgggatcaca tcttcaatcc acggtttatc ggcgaccatg gcttcggtga tgcgatgaat
    19921 cgccatcgct tgtggactaa taggacgatc ggggtgcacc aggtggctca tggggttgac
    19981 gattttcccg tcaatgacat caacagaggc aatctcaacg atccctccct gcaaaccgca
    20041 ggtttctgta tcgataatgc gcaacatggc atgctccagg ccgaaaaagc ctagcgtaat
    20101 ggaatgatat cgccttgcca accctgtgat gaacgctgac cgactaacaa taatgaacca
    20161 cgatcggcgc gaacgatgag acgaccatgt tcatcccata gtgcactgtt accacgggca
    20221 tttgccatca gcacagcaat cgagtactga tgtgaaaaaa attgtaagcg acgggccgat
    20281 gccagcaggt cgggttctcc cagacattgc cctgtcgtaa acagtgaaca ggtcgggtcc
    20341 atatccatac cttgcggttg ttcatcgacc accgtgatgg ttctggaacg acggcccaga
    20401 catgcaccat gactctgatg gtagatccca ggcgtttttc gccacggtgc gaacaccgca
    20461 attccacgaa taaagcgatc gttatattca acgggaaggc cggcaatgat ggtcattcgc
    20521 caggtcgttg cggcataaca aagcgggtct aacagtgaga gatcgggtgg ggcaggaagg
    20581 gcacgtcgcg aataatcaca ccccagtaaa gaaagcgatg gaaaaactaa cagctgacat
    20641 tgttgcctgg cggcggccct cacaaactcc agatgatgag ctacctgttc ggtgagtgag
    20701 gttttacgag gctcatattg cgctgctgca actttccaga acgacataat gacttccttt
    20761 tcaaaatgac tgcaaattat gggtgcggtt tagaaaatac tcataattta gcaggcgtta
    20821 tgtaagaaag tgtaactctg tataagtttt atttaagttt gggctcgtaa ggtaaacgtg
    20881 acagattgac cgaagccaaa cggtgggcaa taagccgctc gcgaaaccag ctgcgcaaat
    20941 gttcaggctg ttcacgttca accgcttcag cgatcactgg catattgtag cgttctttaa
    21001 atgccacccc ggccgccgcc aaatcgacat tcactttatc catttctgtt tgatccagtt
    21061 tagccagatt cttcagcatt cttaatctcc tcatcattcg cgggcaaaag ttactaagag
    21121 tgagcctgga tggcaagccg gaattgatga tcctcgactc agacattaga caaggttatg
    21181 ctctgtaaaa aagttattac aaaaaggaat agataatatg gcttcaactg cacgctccct
    21241 tcgctacgcg ctggcaatcc tgaccacttc gttggtgaca ccttccgtct gggcacatgc
    21301 gcatttaacg catcagtatc ctgcggcaaa cgcgcaagtg acagctgcac cgcaggcaat
    21361 cactttaaac ttctcggaag gtgttgaaac aggattcagt ggtgcaaaaa tcacggggcc
    21421 aaaaaacgaa aatattaaaa cattaccggc gaagcgaaat gagcaggacc aaaagcgact
    21481 gattgttcca ctggccgatt cgctgaaacc cggaacctac accgttgact ggcatgttgt
    21541 ttcggtggac gggcataaaa cgaaaggaca ctacaccttt agcgtgaaat aaatcatgct
    21601 ggcgtttacc tggatcgcgc tgcgatttat ccatttcacc tcgctgatgc tggtctttgg
    21661 cttcgcaatg tacggtgcct ggctggcacc cttaacgatt cgtcgcttgc tgacgaagcg
    21721 ttttctgcgt ttacaacagc acgctgccgt ctggagtttg atcagtgcca ctgcaatgct
    21781 cgccgttcag ggcgggttga tgggaacggg atggaaagat gtattttcgc caaacatttg
    21841 gcaggctgtt ttgcaaacgc agtttggtgg cgtctggtta tggcaaattg ttctcgccct
    21901 cgtaacgttg atcgtcgccc ttatgcaacc gcggaatatg ccacgtttgc tgtttatgct
    21961 cactaccgcg caatttattc tgctggcggg agtggggcat gcgacgctga atgaaggggt
    22021 aacggcgaaa atccatcaga ctaatcacgc gattcacctg atttgtgcgg cggcgtggtt
    22081 tgggggatta ctgccggtgc tctggtgtat gcaactcatc aaaggccgtt ggcgacacca
    22141 ggctattcag gcgctgatgc gtttttcctg gtgcgggcat tttgcggtga tcggcgtact
    22201 ggcaagcggc gtgcttaatg cattgttgat taccggattt ccccccacgc ttacgactta
    22261 ctggggccaa ctcttattgc tcaaagccat cctggtgatg attatggtgg tcattgcgct
    22321 ggctaatcgt tatgttcttg taccgcgcat gaggcaggat gaagatcgtg cggcaccctg
    22381 gtttgtgtgg atgactaaac tcgaatgggc gataggtgcg gtagtgctgc tgatcatcag
    22441 cctgcttgcg actctcgaac ctttttgatg gattacgaaa atgatgaaaa aaagtattct
    22501 ggcgtttctg ttactcacca gttctgcagc ggcgctggct gcaccgcagg tgattaccgt
    22561 cagccgtttt gaagtgggta aagacaaatg ggcgtttaat cgcgaagagg tgatgctgac
    22621 ttgccgaccg ggtaatgctt tgtatgtcat caacccaagt accctcgtgc agtatccttt
    22681 aaacgatgtc gcacagaagg aagttgccag tgggaagact aaagcccaac ccatttcggt
    22741 gattcagatt gatgatccca acaatcccgg cgaaaaaatg agtctggcac cgtttataga
    22801 acgagctgaa aaactctgtt aattacctaa aatagccttt tgatttccaa taaaaaaacc
    22861 gcctcagttc tttcaccaga acgggcggtt tttaacattt aagctgatga ccaccgcgct
    22921 ttttattgac cattttgcac gcaaactgga aaacctggcg tcgtcatcta ttcttaaagg
    22981 gcaaggcaac taagcctgca ttaatgccaa cttttagcgc acggctctct cccaagagcc
    23041 atttccctgg accgaataca ggaatcgtgt tcggtctctt tttatctgtt aaaagccaga
    23101 agcatttcct tcgctgactt tatagtcaac cataacacac actctactgt ctgagtccag
    23161 cgttttttaa cattcttgtt aagattatgt gatctttagc gcgggaggaa aatattgatg
    23221 aaacagcctg cgcccgttta tcagagaatt gcgggtcatc aatggcgaca tatctggctt
    23281 tctggcgata tacacggttg tcttgagcag ttgcgccgca aattatggca ttgtcgtttt
    23341 gatccgtggc gagatttact tatctcagtg ggagacgtta tcgatcgtgg gccgcaaagt
    23401 ttacgttgtc tgcagttact ggaacaacat tgggttcgtg cggtaagagg caatcatgaa
    23461 cagatggcga tggatgcgct ggcatcccag cagatgtctt tgtggttgat gaatggcggc
    23521 gactggttta ttgcgctggc agataatcaa cagaaacaag cgaaaacggc gctggaaaaa
    23581 tgtcagcatt tgccctttat tcttgaagta cacagccgca ccggcaaaca tgttattgct
    23641 catgccgatt atccagatga tgtttatgaa tggcaaaagg acgttgattt gcatcaggtc
    23701 ttgtggagcc gctcgcgatt aagtgaacgc caaaaagggc agggaattac aggtgctgat
    23761 catttctggt ttggtcatac accgttgcgc catcgcgtgg atattggcaa cctgcattat
    23821 attgataccg gcgctgtctt tgggggcgaa ctgactttcg tgcaattgca ataattaaaa
    23881 atcaccatac tcctgtgctg gtcgccagaa accatctata aaatcctcaa tcggaaaaca
    23941 accgccatga cggatccgtt gatcgctcat agaataaaga cactgctgtt ccgtgttgta
    24001 gacatccaca acaatatctt cacaaccgcc atccaggtag caaacaaaaa gtaccagcgc
    24061 gaacatttca tccccgaagt gtggtgccgt accgttaagt ttaggagaga ttttacaacg
    24121 ggggaataac caggacaaat aacccgccat gccgacgggt tctttttgga tcaggcaaga
    24181 cgcataatcc aggcatcaga ttgttgatcg taacgggtac ggtaaaggac agagtgttcg
    24241 ccatttagca gggcagggat actggtttca gcatcccagg catccagttg catacataaa
    24301 tcggggtctg atttacaagg aatggttaac gttcgatcac ccggcgattc accgtgtaat
    24361 tcgccgtcgt cgatttcaaa agcgcctatg cgcacactgg ttttcatctt cgtgctctcc
    24421 atttagctgc cggttgtggt acgaccagtc cgatcgcacc atttttcagc ctagttgagg
    24481 ccgccgaatg tgccagtcaa aaagtgcgct ttttttgtgc gctgtcatgc gttactggta
    24541 aaaagtttcc cgtcccgcac cagttcacgc gggtagctgt ttttcagtcg cgaaccaatc
    24601 cgtttggcta aaccaatcgg ctgatgctgg aaagtaacca acacgtcatc cgccactggc
    24661 gcggcttgcg ggtaaacatc gcgcccgcga taccactcct ccgcttcttg cggtgtcagt
    24721 tcaaaagcgt tctcgttgtc gggggaggca agggcaataa ccgcttcatg ctgccagcga
    24781 taacctttgt tgtgcgtttc ggcaagttta atccccaggc gagaaaatcg gactttaccg
    24841 atcagggctt caatgcctac cggaaacaac cacacttctt tgtcgcgctg ccagagtcgc
    24901 aggtttccat cccagtttaa gccaacactt gcagccgcct gacgaatttg tgcggcttcg
    24961 cgatctttca ccgggctgaa cgggaaattg cccactttgt attttggggt gggtaaggcg
    25021 ggaatggcct gagttttacg cagacgagca acgaagaagc cttcgcagtc gtaaatttgt
    25081 gggaaaacat gcaaaaagcc ttcttcggtc agcgctttgt ttgcaccagg gaagagatcg
    25141 ccaagtggta aaaactctac tgcgtcgggg taagtctctt taagccacag gcaaacggat
    25201 tcgttttctt cccggtttaa ggtacaggtc gagtaaacca gtgtaccgcc agagcgtaat
    25261 gcatgaaagg cactgtcgat cagttcccgt tgggtagctg cgatttcctg attgctttct
    25321 ggtgaccaat tttttagcgc atcgggatct ttacgcacca cgccttcgcc ggagcaggga
    25381 gcgtccagca aaatggcatc gaacatttct ggcaatgccg caccaaacac gcggccatca
    25441 aaatgtgtga gcgcaacatt actgatacca cagcggctga tattggcatg taacactttt
    25501 acccgactgg cggaaaactc attggcaagg attgcccctt cgttattcat ccgcgcggca
    25561 atttgcgtcg ttttggagcc tggcgcagca gcgacatcca tcacccgctg tggtgcatta
    25621 ccgtcagcaa acaaggcggc gacgggcaac attgaactgg cttcctgaat ataaaacagg
    25681 ccacttaaat gctcggcggt actacccaat ggcaatgcat cttcatcgtc gcgttcaatc
    25741 cagaaacctt cttcacacca cggaattggc gtaagcgtcc agccataagg agcggttaat
    25801 cgcaggaaat cagcaacgga ggttttcagc gtattaacgc gaatgctgcg gcgcaacggg
    25861 cgctgacagg cggcaagaaa atcatcaaaa gagagcgtcg aaggcattgc ttcgcgcatt
    25921 tgcgtcagaa aggcgtccgg gaaataaacg gtgtgttggg ccacgagcat ataccactgg
    25981 caaaaaaaac aggcgcgcag tttagcataa acgctccggc gcaggcacgc cggagcagtg
    26041 ggcattattt gggaagcgca gttccccatt cacgccactc tttcggttca ctttcctgca
    26101 acaggaagtg cttgccttcc tgggctttcg gtgccagtgg cgtacccgga ggcgtggcga
    26161 aggcgatgcc gccacggata aactggttaa aggtgccggt tttcactacg ccgcccgtca
    26221 gaccaaagtc cagactgtaa cccgatgcca accagaagac ggaattgtta cgcaccaggt
    26281 gttgatagcg tttactgatg cgcatcgcaa tcatcacgcg atctgacaat gtccccagcg
    26341 tcattccggt aaccgtaccg acttccagac cacggaacag cacaggcgta ccgatgccta
    26401 acgaacaggc ttccggcgct tcaacaataa tgcttaaacc atccaggtaa cgcgaatcag
    26461 taatggtggc ctcttgtaat tcaaagtcgc gacgaggatt gccacggcct ggttcgacgt
    26521 tgatatacgg ctggaggata gtatcaagat gctcaacgcc agctgccgaa atttgcggtg
    26581 tgaccactga gaagcgcgta ccaccgcgag caaaggtctg gacatattcc ggatagagca
    26641 ccgcctttgc ctgtacttca ttgcgcgcgg taatcagatc cagcgtctgg atttgcccga
    26701 tatcaatacc gagatagcga attggcatcc cgaccgccag ttttccggca tcgaaagcat
    26761 gaagcgtaat ctgcccgcca accgcacggg ccgctgtttc ggaagcatac agaatacgct
    26821 tatcgccttt acgctgactg gcgctggcac cgctgaggtt atcgaagcta atggctccct
    26881 ttaatgctct ggagagtggg gatgcctgta cggtcaggcc actaccattc agctgaactt
    26941 tcgccccgcc ttctgcccag aacacgctat tgttggtcag aaggttgcga tactccggct
    27001 taatatgcag atcgatatca aacgcgttag ctcgcggacg cacggtaata acttcaccaa
    27061 cttcaaattt acggtacagc actaccgatc ctgcctgcac atccggcagc gtctctgcac
    27121 tcaaactcac ggttgtggtg ggtaaatcgc taaggctgtt ctccagcgct ttttccagat
    27181 tggcatacag tggatagctg gctttcatct cacctttatc gcccggcaga atacgtatcc
    27241 cgccattgat ccattctgag gcgctggcac cgagaaactc aacgccatcc agccccacct
    27301 tcacatcgac acggctgttg acgacaaatt tgctatcgcc ttttaccagt tcgcgatgct
    27361 gaggctcaat ggcgacggta aaggtaacgc ctttgctggt gagtttacga tcaataacct
    27421 ggcctacctg cacgccgtga agaatgagcg gctgaccagc atcaataccg taactttccg
    27481 gtgctgtcag ggtcagtgtc agaacatcag gttcatgcag cagtgctttt tcgcctggca
    27541 caacaacgaa ctctttgcgt ggctcgccat cgccaggcac caactcaaag gtttttccgg
    27601 tcagcagggc gctgagattg gcatcgctaa gggataattt cgggttgcgt aattcgatgc
    27661 gggtattttc ccgtaacagc gtaacgacgc tgggatcaac ggtcatttct ccggtgactt
    27721 taccaccagg atttaaatcc agtttagtca gctgtccgac ttccagcccc tgatacatta
    27781 acggcgtcga gtcggcggtt aatccggccc cactcggcag ttccagtttt attattacgc
    27841 cacgctggct gtgggccaga tcttcataca gaccaaaggt atcttccgcc tcggcaggtt
    27901 tcgactcttc tggtgaatcg aaggcaatcg caccgttaac cagtgccgcc agactttcca
    27961 gtttcacctt cgcgccactg atactgacgt tggcatcaac gcctgaaacg ttccagaaac
    28021 ggctaccttt tttcaccagg tcggtaaaac gccgctcgat caggacatca atcaccacgc
    28081 cttgtttatt gggattgatg gcatagtcgt agacttttcc caccgggatc ttgcggaaat
    28141 agaccaatga accgctgttc agcgaaccga gatcgggagc ttgcaggtgg atcatcagat
    28201 cgccgttatc cagccgatac ttcggttggg tatcgagcgc gacaaagtga tcctgctctt
    28261 tacctttacc tggcatcatg ccgatatagt taccaccgac gagggcgtcc agcccggaga
    28321 cacctgccaa cgatgctttt ggcgtcacca gccagaactg agtctcttcg cgcagcgcat
    28381 ctttcatatc ggacttgatg ctgaccttga cttcaatctt acgaagatcg tcgctgaggc
    28441 tgatatcctg cactgttccg acttcaacgc cctgataacg aacaggcgta cggcccggga
    28501 caataccatc cgccgacata aagtcgatgg tgacggtatt accccggtcc tgataactgt
    28561 cccaaatcag ccaaccggca atcattagcg cgatgaacgg cagcagccag aaaggtgaga
    28621 tacggcgttt atttttaatc tgcgcttcag tcgtcgaagc gggcgtttcc tgactcatgt
    28681 gcatcccaaa gtaagcggct gtccagccat tccacagcaa gaatagtcaa aattaccgct
    28741 gcgccgaaat aaaacgcagc cggtcccata gtaaaagcga ggatctgatc gcgattaatc
    28801 agcgacatgg ttaaagatat gacaaacagg tcgagcatcg accagcgacc aatccaggtc
    28861 accatccgca gtaacagaat gcgtgtgcgt aaaccttgct ggcatttaaa atgaatgctc
    28921 aacagtaaag tgaacatgac gatcacttta gtaaacggta ccagaatact ggcgataaac
    28981 acgattcctg caaccgcaat gttgctactt gccagcgaca taattccgga aagaattgta
    29041 tcttcctgcc gtcctccgtt cagataaatg atagaaatag gcaacaggtt ggcaggtaac
    29101 aataaaacga ttgacgctaa cagcgccgcc cagcattttt gcagactatg acggcgacgc
    29161 aggcgtagcg gaatatgaca acgcgggcag cgaccacgct gatcaggata gccggtaaaa
    29221 tggcacccaa gacagacacg aagtttctcg tccctacgcg tagcggggcg ctgcggataa
    29281 aatcgctccc acagctcctc gacattaaga tgtgacaacg tcaccgtcgt taaaatcacc
    29341 aacgccacaa aagagaacaa gccgacaccc gcctggatat gggcataatc ctgtaccttt
    29401 atagaagcaa cgccaatgcc gaccaggtag atatcgagca ttacccactc tttaagtcgc
    29461 tcaagcatca gcagtaccgg gcgtagattc attcccagtc ggttaccaaa ccataaataa
    29521 gctatggagg tcaccagaat gagcggggca ccaataacgc agaaaaagac catcgacccc
    29581 gttatcgcat cgccctgttt ggtcatttgc cagatgcctt gcataacgtt ggcgtcaata
    29641 cgaatgccta acagccagat atgcaacagc ggttcgcccc aggcaaacgg catcagcaac
    29701 agcatggtga aggccattgc cgccaggcgc gttagcgacc agtcgcgccc gtcacgaatt
    29761 tttgcctgac agcgcggaca ataggcactt tgatgagaat ttatctcggg caggctaaac
    29821 agcatgtcac attgcgggca acgttggtaa tcaccacgcg gcagttcctc gccgattgcc
    29881 ctcactgtta tcttttttgt cggcgtaatt tgtggtgtgt taagagccat gacgtgccaa
    29941 atgtaataag aattaacatt aatcttaacc catgatggga atgatcttga gcattaacgc
    30001 atttaatgct tattttaata ggctggcagt tccaggtaag acaaccaagt gattatataa
    30061 tgaacaaaac agaattttac gcggatttaa atcgcgactt taacgcgctg atggcgggag
    30121 aaaccagttt tctggcaacg cttgcgaaca ccagtgcgtt gttatatgag cgtctcactg
    30181 acgtaaactg ggcaggtttt tatttgcttg aggacgatac actggtgctc ggaccatttc
    30241 agggcaaaat tgcctgtgtc cggatacccg tcgggcgtgg cgtgtgcggc actgcggttg
    30301 cccgcaatca agtgcagcgt atcgaggatg ttcatgcgtt tgacgggcat attgcctgtg
    30361 atgcggcgag taattctgaa attgttctgc cgctggtggt gaaaaatcag attattggtg
    30421 ttctcgacat cgatagtacc gtcttcggtc gctttacaga cgaggacgag caaggcttac
    30481 gtcagcttgt ggcacagctt gaaaaagtgc ttgcaacgac ggattacaaa aaattctttg
    30541 cgagcgtcgc aggataatca acggataacg tagcatttac tgatagcgtc attataatga
    30601 cgcctgttca tgcctgcgct tgttggctac gtccgttgta atcaggaaat ttcatggaaa
    30661 atcaacctaa gttgaatagc agtaaagaag taatcgcgtt tctggccgaa cgttttcccc
    30721 actgtttcag tgcggaaggt gaagcgcgtc cgctgaaaat cggtattttt caggatttgg
    30781 tcgatcgtgt tgccggggaa atgaacctga gcaaaacgca attacgatcc gctttacgtc
    30841 tctacacttc gagctggcgt tacctttacg gtgttaaacc cggcgcaacg cgtgtcgatc
    30901 ttgacggcaa cccatgcggt gagctggacg agcaacatgt agagcatgct cgcaagcagc
    30961 ttgaagaagc gaaagcgcgt gttcaggcac agcgtgctga acagcaagcg aaaaaacgcg
    31021 aagctgccgc agctgctggt gagaaagaag acgcaccgcg tcgcgaacgc aagccacgtc
    31081 cgaccacgcc acgccgcaaa gaaggcgctg aacgtaaacc tcgtgcgcaa aagccggtag
    31141 agaaagcgcc aaaaacagca aaagcacctc gcgaagaaca gcacacccca gtttctgaca
    31201 tttcagctct gactgtcgga caagccctga aggtgaaagc gggtcaaaac gcgatggatg
    31261 ccaccgtatt agaaatcacc aaagacggcg tccgcgtcca gctgaattcg ggtatgtctt
    31321 tgattgtgcg cgcagaacac ctggtgttct gaaacggagg ccgggccagg catgaacatg
    31381 ttttttaggc ttaccgcgtt agctggcctg cttgcaatag caggccagac cttcgctgta
    31441 gaagatatca cgcgtgctga tcaaattccg gtattaaagg aagagacgca gcatgcgacg
    31501 gtgagtgagc gcgtaacgtc gcgcttcacc cgttctcatt atcgccagtt cgacctcgat
    31561 caggcatttt cggccaaaat ctttgaccgc tacctgaatc tgctcgatta cagccacaac
    31621 gtgctgctgg caagcgatgt tgaacagttc gcgaaaaaga aaaccgagtt aggcgatgaa
    31681 ctgcgttcag gcaaactcga cgttttctac gatctctaca atctggcgca aaagcgtcgt
    31741 tttgaacgtt accaatacgc tttgtcggta ctggaaaagc cgatggattt caccggcaac
    31801 gacacttata accttgaccg cagcaaagcg ccctggccga aaaacgaggc tgagttgaac
    31861 gcgctgtggg acagtaaagt caaattcgac gagttaagcc tgaagctggc aggaaaaacg
    31921 gataaagaaa ttcgtgaaac cctgactcgc cgctacaaat ttgccattcg tcgtctggcg
    31981 caaaccaaca gcgaagatgt tttctcgctg gcaatgacgg cgtttgcgcg tgaaatcgac
    32041 ccgcatacca actatctttc cccgcgtaat accgaacagt tcaacactga aatgagtttg
    32101 tcgctggaag gtattggcgc agtgctgcaa atggatgatg actacaccgt tatcaattcg
    32161 atggtggcag gtggtccggc agcgaagagt aaagctatca gcgttggtga caaaattgtc
    32221 ggtgttggtc aaacaggcaa gccgatggtt gacgtgattg gctggcgtct tgatgatgtg
    32281 gttgccttaa ttaaagggcc gaagggcagt aaagttcgtc tggaaatttt acctgctggt
    32341 aaagggacca agacccgcac tgtaacattg acccgtgaac gtattcgtct cgaagaccgc
    32401 gcggttaaaa tgtcggtgaa gaccgtcggt aaagagaaag tcggcgtgct ggatattcct
    32461 ggcttctatg tgggtttgac agacgatgtc aaagtgcaac tgcagaaact ggaaaaacag
    32521 aatgtcagca gtgtgatcat cgatctgcgt agcaatggtg gtggggcgct gaccgaagcg
    32581 gtatcgctct caggtctgtt tattcctgcg ggtcctattg ttcaggtccg tgataacaac
    32641 ggtaaagttc gtgaagacag cgataccgac ggacaggttt tctataaagg cccgctggtg
    32701 gtactggttg accgcttcag tgcttcggct tcagaaatct ttgccgcggc aatgcaggat
    32761 tacggtcgtg cactggttgt gggtgaaccg acgtttggta aaggcaccgt tcagcaatac
    32821 cgttcattga accgtattta cgatcagatg ttacgtcctg aatggccagc gctgggttct
    32881 gtgcagtaca cgatccagaa attctatcgc gttaacggcg gcagtacgca acgtaaaggc
    32941 gtaacgccag acatcatcat gccgacgggt aatgaagaaa cggaaacggg tgagaaattc
    33001 gaagataacg cgctgccgtg ggatagcatt gatgccgcga cttatgtgaa atcaggcgat
    33061 ttaacggcct ttgaaccgga gctactgaag gaacataatg cgcgtatcgc gaaagatcct
    33121 gagttccaga acatcatgaa ggatatcgcg cgcttcaacg ctatgaagga caagcgcaat
    33181 atcgtttctc tgaattacgc tgtgcgtgag aaagagaata atgaagatga tgcgacgcgt
    33241 ttggcgcgtt tgaacgaacg ctttaaacgc gaaggtaaac cggagttgaa gaaactggat
    33301 gatctaccga aagattacca ggagccggat ccttatctgg atgagacggt gaatatcgca
    33361 ctcgatctgg cgaagcttga aaaagccaga cccgcggaac aacccgcacc cgtcaagtaa
    33421 tatcaatcag gcacaagaaa ttgtgcctga ttttttaaca gcggcaagat gccgtaaatc
    33481 agatgctaca aaatgtaaag ttgtgtcttt ctggtgactt acgcactatc cagacttgaa
    33541 aatagtcgcg taacccatac gatgtgggta tcgcatattg cgttttgtta aactgaggta
    33601 aaaagaaaat tatgatgcga atcgcgctct tcctgctaac gaacctggcc gtaatggtcg
    33661 ttttcgggct ggtactgagc ctgacaggga tacagtcgag cagcgttcag gggctgatga
    33721 tcatggcctt gctgttcggt tttggtggtt ccttcgtttc gcttctgatg tccaaatgga
    33781 tggcattacg atctgttggc ggggaagtga tcgagcaacc gcgtaacgaa agggaacgtt
    33841 ggctggtcaa tactgtagca acccaggctc gtcaggcggg gatcgctatg ccgcaagtgg
    33901 ctatctacca tgcgccggac atcaacgctt ttgcaaccgg tgcgcgccgt gatgcctctc
    33961 tggttgctgt cagcaccggt ttgctgcaga acatgagccc ggatgaagcc gaggcggtaa
    34021 ttgctcacga aatcagccac atcgccaatg gtgatatggt caccatgacg ctgattcagg
    34081 gcgtggtgaa caccttcgtt atctttattt cccgtatcct ggcgcagctt gccgcgggtt
    34141 ttatgggcgg aaatcgtgat gaaggtgaag agagcaacgg caacccgctg atctactttg
    34201 cggttgcaac ggttctggaa ctggtgtttg gtattctggc gagcattatc accatgtggt
    34261 tctcgcgtca tcgtgaattc catgctgatg ccggttcggc aaaactggtt ggtcgcgaga
    34321 aaatgattgc cgcgctgcag cgcctgaaaa ccagctatga accgcaagaa gcaaccagca
    34381 tgatggcttt ctgcattaac ggtaagtcga aatcgctcag tgagttgttt atgactcacc
    34441 cgccgctgga taaacgtatt gaagctctgc gtacgggtga atacctgaag taatatgaag
    34501 taaatactaa aaagcgcgtc ctgctcgacg cgcttttttt atgccctgga tcttggctga
    34561 gtgatacgta acccactgat acaggcagca atcactgcca gaatagccgc agccatcagc
    34621 gagacgtgtg taccattatc tccaaactga tttagcatca gcgccaccag cgccgcgccg
    34681 ctactctgac ccagtagacg agccgttcct aacatgccac tggctccacc gctacgttcg
    34741 cgaggcgcgg aggtaataat ggtgtggtta ttgggtgact ggaacaagcc aaatccagcg
    34801 ccacataaga tcatcggcca gataatattg atatccgcag gtgacgcggg cagcagaacc
    34861 agggaaaaaa gccccgcagc catgatgaat aatcctaaag cacccagcaa tcctgcatgt
    34921 acgcgttcaa tcaaatagcc tgccaacgga gccatcacca tcgttgctaa cggccacggt
    34981 gtcagaagta aacctgtttc gacttcacta cgcccgagca cggtttgcag gtaaaagggc
    35041 agggaaacca ttgccagcat ttgtgcgcag aaagagcaaa cagatgtgca aatagaaagt
    35101 gaaaacagcg ggatacgcag taaatccacc ggtagcagcg gtactggaag agaaagctgg
    35161 cggcgaataa agaaaatacc aacgacaacc attacaacca gttccgcagc aattaacgtc
    35221 agcgattgcc cctgagcgaa accactcaac gcagtgataa gcaggccgaa ggttaacgcg
    35281 ttcatcacgg cgctgggcag gtcgaaacgg ggtttactgg cgcgagaacc attgggtggc
    35341 agaaaacgca tcgccagaag cagggcgata atacccaacg gtacgttgat taaaaataac
    35401 catttccagg atgcgatgga gaggattgct gcagcaattg tcggcccggc agcagaagag
    35461 acggcaacaa taaacgagtt tatgcccatc cctctaccca gaaaacgttg tggatagatc
    35521 aggcggataa gggcggtatt aacgctcatc aacgctgcac cgccgaaacc ttgtatgaca
    35581 cgcgcaaggg tgagtatttg cagcgaatca gaaagggcgc agaacagtga agacaacaga
    35641 aaaacgacca gaccgcattt ataaatacgt cgatagccaa acatatcacc cagaaacgaa
    35701 aacgagagca gggagatgac aatggcgatt tgataggcgt tcactaccca gatggaactg
    35761 gctggcgtgg catgaaggtc cgtggcgatt gttggcaggg cgacgttggc gattgcgccg
    35821 tcaaggacag ccatcgaaat accaatcaca atggttaata tcgcaccgta tcgctggggc
    35881 aatggcaggc cgtcggcctg aacttttggc ataggaattt taaatctttg gtgaataatc
    35941 aacaggggta tgattctagc actgatattc acggtggatg tcgcagaatt gtaactaacc
    36001 ggcaaaagat ggattgcgac caccctggtg cgaaattata ataaaaaaca gttctgattt
    36061 ttataaaaca ctcgcaatga ggtgataaat ggctaacgca gatctggata aacagcctga
    36121 ttctgtatct tccgtgctaa aagtttttgg cattttgcag gcgctgggtg aagagcgcga
    36181 aatagggata accgagctgt cgcagcgcgt catgatgtca aaaagcaccg tttatcgctt
    36241 tttacagacc atgaaaacct taggttatgt ggcgcaggaa ggggagtcgg agaaatattc
    36301 cctgaccctg aaattgtttg aactgggcgc tcgcgcgtta caaaacgtcg atttaattcg
    36361 tagcgcagat atccagatgc gtgagatctc ccgcctgacc aaagaaacta tccacctcgg
    36421 cgcactggac gaagacagta ttgtttacat tcacaaaatt gactctatgt acaatttgcg
    36481 catgtattca cggattgggc gtcgtaatcc actgtacagc accgcgattg gtaaggtact
    36541 gctggcatgg cgcgatcgcg atgaagtgaa gcaaattctt gagggcgtgg agtataaacg
    36601 cagtaccgag cggaccatca ccagtacaga agcgttatta ccggttctgg accaggtgcg
    36661 cgagcagggg tatggcgaag ataacgaaga gcaggaagaa gggctgcgat gcattgcggt
    36721 tccggtattt gatcgctttg gcgtggtcat tgccggtttg agcatctcct tcccgacgtt
    36781 gcgtttctct gaagagcgtt tacaggaata tgtcgcaatg ttgcataccg cagcgcgcaa
    36841 aatttctgcc caaatgggtt atcacgacta tccgttctga tgagagtaag aacctgtcgg
    36901 aatatcaaac agacaggttc tttatttagc atgagaaaaa taaagttgaa ggtggcgtta
    36961 gattaaacgc agttgatata agaatatttt actcatgagt gagaatctgg ttatttatta
    37021 cacttaacca ttatcgacca cgatattgct tttgcgtaac agcgggcaat ctgttatccc
    37081 caaaaaacca cttttagtgt gcaagtattg taccgtgctg gtgcctctgg cagtcagata
    37141 ggtacattgc aaacctaatt ctgcggcatt ctctttgctt ccaatcaaaa cgccatatcc
    37201 gctgagtaat aatcctatcc ataccagtgc tatcagcata actgtgcgaa tgatgaatcg
    37261 cattacaacc tcttctcttt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    37321 aagcgactca ttccgaaaaa gcacgaatat cgacatagtt agacgctgtt taactaacgc
    37381 atggtagttt aatgacataa ggtaggtgaa acggagattg gagtgaaaaa gtttcgatgg
    37441 gtcgttttgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    37501 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    37561 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    37621 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    37681 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttattaatc
    37741 gtgccagttc tcggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    37801 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    37861 aaaagactat tgagagtcaa aaatcggttg aagacgaaga tattattcgc ctggtcgccg
    37921 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    37981 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    38041 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt accccattaa
    38101 tttattagga tgtttacatc ggatttgtga ttaagcgtgg tattatttat tagcgaaacg
    38161 tttctctctt gatttttttt gcttattcat caatttttct tattttaaat ttccaattcc
    38221 tttgggattg acttctcttt ggggtgattg agagccgtta actgcctgtt ttatgagaaa
    38281 aagtgatata actttttatt cattgcatag cagaaaatgt gatattgcac gcactatgta
    38341 ataacttctc ccactgacct ggaacaactg aacttattga actatgttag aaactacgtc
    38401 agtttaagta tctgcggaac tgacaaggtt aagcacaatg atatatcggc gcgtattccg
    38461 ttgcataagt gtgcaaaaaa agtggaagac gtatcgagat ttgtgcgtct gatcgagaca
    38521 tgtttaaaaa tggcttgcca taattaacgt tgtatgtgat aacagatttc gggttaaacg
    38581 aggtacagtt ctgtttatgt gtggcatttt cagtaaagaa gtcctgagta aacacgttga
    38641 cgttgaatac cgcttctctg ccgagcctta tattggtgcc tcatgcagta atgtgtcagt
    38701 tttatctatg ttatgcctgc gggcgaagaa aacaatctaa ggaatttttc aaatggcaaa
    38761 gattaaaggt caggttaagt ggttcaacga gtctaaaggt tttggcttca ttactcctgc
    38821 tgatggcagc aaagacgtgt tcgtacactt ctccgctatc cagggtaatg gcttcaaaac
    38881 tctggctgaa ggtcagaacg ttgagttcga aattcaggac ggccagaaag gtccggcagc
    38941 tgttaacgta acagctatct gatcgaatcc actgatctga agtgtgaata cgcttcaatc
    39001 tcgctataaa gcctcgtcga atgcgaggct ttttactatg ctttatcttc gctcctggcg
    39061 ttcggatatt tgcccgccgc gtgattcgcg ttacacttgc ggcctttagt atcctgccgg
    39121 agttgtcatg tcgttttcct gtccactttg ccatcagcct cttacgcgtg aaaaaaacag
    39181 ctatatctgt ccccaacgac atcagtttga tatggcgaaa gaagggtatg tcaatctgct
    39241 gcccgttcag cataaacggt ctcgtgatcc gggcgacagc gcggaaatga tgcaagcacg
    39301 ccgcgcattc ttagatgccg gacattatca gccgctgcgt gatgcaattg tcgcccaact
    39361 gcgggaacgg cttaatgaaa aggccacggc ggtgctggat attggctgtg gtgaagggta
    39421 ttacacacac gcatttgccg atgcgttgcc cgaaatcacc acgtttggtc tggatgtttc
    39481 gaaggtagcg ataaaagcgg cggcgaaacg ctatccgcag gtcactcttt gtgtcgcttc
    39541 cagccaccgt ttgccgtttt ccgataccag tatggacgcc ataatacgta tttacgcgcc
    39601 gtgtaaagca gaagaattag cacgagtagt gaagcccggc ggctgggtca ttactgccac
    39661 gccgggaccg cgacatttga tggagctgaa ggggctgatt tacaatgaag tacatcttca
    39721 tgcacctcat gcagaacaac tggaaggttt tacattacag aagagtgatg agttgtgtta
    39781 tccgatgcgt cttcgcggtg atgaagccgt cgcattattg cagatgacgc cgtttgcctg
    39841 gcgtgcgaag ccagaagtct ggcaaatact ggcagcaaaa gaagtgttcg actgccagac
    39901 ggactttaat attcacctct ggcagcgttc ttattaaccg tggaagtgcg tccagaggat
    39961 ctggacgccg atgccgatca gcaccagccc gccgagaatt tccgcttttt tcccaataat
    40021 tgagccgata aagcgaccaa ccatcatccc taatgtcgac ataatcaagg ttgcacaacc
    40081 aatggccaat gcggtcgcga taatgttgac ctgaaggaaa gcaagaccaa cacccacagc
    40141 catggcatcc aggctggtgg caatcgcggt ggttaccagt agccagaaac cgtgccgacg
    40201 gcgcggctct tcatcttcat catctgcgcc acgaaaaccc tcaataatca ttcgcccgcc
    40261 gaggaatatc agcagcacaa acgcaatcca gtgattccat tcaaggacaa accggctggc
    40321 taacatgccc attccccagc cgatcagcgg cgtcagggtt tcgacggcac caaaaataag
    40381 gccggttcgc aatgcttcag aaaatttcgg tttatggagg gtggcacctt taccgattga
    40441 tgcagcaaat gcatccatcg acataccaaa cgcaagaaga acagtagcag tgatattcat
    40501 gacaacgtcc tgaccggggt atccataaaa cacatcatcg cccccagcaa acatgcatac
    40561 atgaacaaca agccgcgatg ggttttcgcg ttcatgcagg catagcgatt gatgtgcata
    40621 tggtctcgcc tgactgaaag tcttcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
    40681 gacacgtaca tttccggaat ctggaaattg gctactcccc aa
//
LOCUS       DAZFBR010000028        40673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFBR010000028 DAZFBR010000000
VERSION     DAZFBR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.028"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            117..1685
                     /gene="cydA"
                     /locus_tag="ACHJZ2_001716"
     CDS             117..1685
                     /gene="cydA"
                     /locus_tag="ACHJZ2_001716"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO4567849.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1701..2840
                     /gene="cydB"
                     /locus_tag="ACHJZ2_001717"
     CDS             1701..2840
                     /gene="cydB"
                     /locus_tag="ACHJZ2_001717"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4567850.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            2855..2968
                     /gene="cydX"
                     /locus_tag="ACHJZ2_001718"
     CDS             2855..2968
                     /gene="cydX"
                     /locus_tag="ACHJZ2_001718"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO4567851.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            2968..3261
                     /gene="ybgE"
                     /locus_tag="ACHJZ2_001719"
     CDS             2968..3261
                     /gene="ybgE"
                     /locus_tag="ACHJZ2_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO4567852.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3411..3815
                     /gene="ybgC"
                     /locus_tag="ACHJZ2_001720"
     CDS             3411..3815
                     /gene="ybgC"
                     /locus_tag="ACHJZ2_001720"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO4567853.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3812..4504
                     /gene="tolQ"
                     /locus_tag="ACHJZ2_001721"
     CDS             3812..4504
                     /gene="tolQ"
                     /locus_tag="ACHJZ2_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO4567854.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4508..4936
                     /gene="tolR"
                     /locus_tag="ACHJZ2_001722"
     CDS             4508..4936
                     /gene="tolR"
                     /locus_tag="ACHJZ2_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO4567855.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5001..6185
                     /gene="tolA"
                     /locus_tag="ACHJZ2_001723"
     CDS             5001..6185
                     /gene="tolA"
                     /locus_tag="ACHJZ2_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO4567856.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSS
                     GKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGK
                     TCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDF
                     KP"
     gene            6318..7610
                     /gene="tolB"
                     /locus_tag="ACHJZ2_001724"
     CDS             6318..7610
                     /gene="tolB"
                     /locus_tag="ACHJZ2_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO4567857.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7645..8166
                     /gene="pal"
                     /locus_tag="ACHJZ2_001725"
     CDS             7645..8166
                     /gene="pal"
                     /locus_tag="ACHJZ2_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO4567858.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8176..8967
                     /gene="cpoB"
                     /locus_tag="ACHJZ2_001726"
     CDS             8176..8967
                     /gene="cpoB"
                     /locus_tag="ACHJZ2_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO4567859.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9132..9207
                     /locus_tag="ACHJZ2_001727"
     tRNA            9132..9207
                     /locus_tag="ACHJZ2_001727"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9165..9167,aa:Lys,seq:ttt)
     gene            9243..9318
                     /locus_tag="ACHJZ2_001728"
     tRNA            9243..9318
                     /locus_tag="ACHJZ2_001728"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9276..9278,aa:Val,seq:tac)
     gene            9321..9396
                     /locus_tag="ACHJZ2_001729"
     tRNA            9321..9396
                     /locus_tag="ACHJZ2_001729"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9354..9356,aa:Lys,seq:ttt)
     gene            9448..9523
                     /locus_tag="ACHJZ2_001730"
     tRNA            9448..9523
                     /locus_tag="ACHJZ2_001730"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9481..9483,aa:Val,seq:tac)
     gene            9527..9602
                     /locus_tag="ACHJZ2_001731"
     tRNA            9527..9602
                     /locus_tag="ACHJZ2_001731"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9560..9562,aa:Lys,seq:ttt)
     gene            9749..9824
                     /locus_tag="ACHJZ2_001732"
     tRNA            9749..9824
                     /locus_tag="ACHJZ2_001732"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9782..9784,aa:Lys,seq:ttt)
     gene            10102..11145
                     /gene="nadA"
                     /locus_tag="ACHJZ2_001733"
     CDS             10102..11145
                     /gene="nadA"
                     /locus_tag="ACHJZ2_001733"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO4567860.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11183..11902
                     /gene="pnuC"
                     /locus_tag="ACHJZ2_001734"
     CDS             11183..11902
                     /gene="pnuC"
                     /locus_tag="ACHJZ2_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO4567861.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11899..12840)
                     /gene="zitB"
                     /locus_tag="ACHJZ2_001735"
     CDS             complement(11899..12840)
                     /gene="zitB"
                     /locus_tag="ACHJZ2_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO4567862.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(12954..13334)
                     /gene="ybgS"
                     /locus_tag="ACHJZ2_001736"
     CDS             complement(12954..13334)
                     /gene="ybgS"
                     /locus_tag="ACHJZ2_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO4567863.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13650..14702
                     /gene="aroG"
                     /locus_tag="ACHJZ2_001737"
     CDS             13650..14702
                     /gene="aroG"
                     /locus_tag="ACHJZ2_001737"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO4567864.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(14868..15620)
                     /gene="gpmA"
                     /locus_tag="ACHJZ2_001738"
     CDS             complement(14868..15620)
                     /gene="gpmA"
                     /locus_tag="ACHJZ2_001738"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO4567865.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(15822..16862)
                     /gene="galM"
                     /locus_tag="ACHJZ2_001739"
     CDS             complement(15822..16862)
                     /gene="galM"
                     /locus_tag="ACHJZ2_001739"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO4567866.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            complement(16856..18004)
                     /gene="galK"
                     /locus_tag="ACHJZ2_001740"
     CDS             complement(16856..18004)
                     /gene="galK"
                     /locus_tag="ACHJZ2_001740"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO4567867.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18008..19054)
                     /gene="galT"
                     /locus_tag="ACHJZ2_001741"
     CDS             complement(18008..19054)
                     /gene="galT"
                     /locus_tag="ACHJZ2_001741"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO4567868.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19064..20080)
                     /gene="galE"
                     /locus_tag="ACHJZ2_001742"
     CDS             complement(19064..20080)
                     /gene="galE"
                     /locus_tag="ACHJZ2_001742"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO4567869.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20342..21814)
                     /gene="modF"
                     /locus_tag="ACHJZ2_001743"
     CDS             complement(20342..21814)
                     /gene="modF"
                     /locus_tag="ACHJZ2_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO4567870.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            complement(21882..22670)
                     /gene="modE"
                     /locus_tag="ACHJZ2_001744"
     CDS             complement(21882..22670)
                     /gene="modE"
                     /locus_tag="ACHJZ2_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO4567871.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            22799..22948
                     /gene="acrZ"
                     /locus_tag="ACHJZ2_001745"
     CDS             22799..22948
                     /gene="acrZ"
                     /locus_tag="ACHJZ2_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO4567872.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23115..23888
                     /gene="modA"
                     /locus_tag="ACHJZ2_001746"
     CDS             23115..23888
                     /gene="modA"
                     /locus_tag="ACHJZ2_001746"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4567873.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            23888..24577
                     /gene="modB"
                     /locus_tag="ACHJZ2_001747"
     CDS             23888..24577
                     /gene="modB"
                     /locus_tag="ACHJZ2_001747"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO4567874.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            24580..25638
                     /gene="modC"
                     /locus_tag="ACHJZ2_001748"
     CDS             24580..25638
                     /gene="modC"
                     /locus_tag="ACHJZ2_001748"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO4567875.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(25639..26457)
                     /gene="ybhA"
                     /locus_tag="ACHJZ2_001749"
     CDS             complement(25639..26457)
                     /gene="ybhA"
                     /locus_tag="ACHJZ2_001749"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO4567876.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            26612..27607
                     /gene="pgl"
                     /locus_tag="ACHJZ2_001750"
     CDS             26612..27607
                     /gene="pgl"
                     /locus_tag="ACHJZ2_001750"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO4567877.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(27648..28601)
                     /gene="ybhD"
                     /locus_tag="ACHJZ2_001751"
     CDS             complement(27648..28601)
                     /gene="ybhD"
                     /locus_tag="ACHJZ2_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4567878.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            28785..29837
                     /gene="ybhH"
                     /locus_tag="ACHJZ2_001752"
     CDS             28785..29837
                     /gene="ybhH"
                     /locus_tag="ACHJZ2_001752"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO4567879.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            29913..31346
                     /gene="ybhI"
                     /locus_tag="ACHJZ2_001753"
     CDS             29913..31346
                     /gene="ybhI"
                     /locus_tag="ACHJZ2_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO4567880.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            31529..33790
                     /gene="ybhJ"
                     /locus_tag="ACHJZ2_001754"
     CDS             31529..33790
                     /gene="ybhJ"
                     /locus_tag="ACHJZ2_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO4567881.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(33931..35214)
                     /gene="ybhC"
                     /locus_tag="ACHJZ2_001755"
     CDS             complement(33931..35214)
                     /gene="ybhC"
                     /locus_tag="ACHJZ2_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO4567882.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35349..36415)
                     /locus_tag="ACHJZ2_001756"
                     /pseudo
     CDS             complement(35349..36415)
                     /locus_tag="ACHJZ2_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(36393..36611)
                     /locus_tag="ACHJZ2_001757"
     CDS             complement(36393..36611)
                     /locus_tag="ACHJZ2_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4567883.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(36651..36818)
                     /locus_tag="ACHJZ2_001758"
     CDS             complement(36651..36818)
                     /locus_tag="ACHJZ2_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567884.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            37061..37663
                     /locus_tag="ACHJZ2_001759"
     CDS             37061..37663
                     /locus_tag="ACHJZ2_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567885.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVLGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            complement(37874..38095)
                     /locus_tag="ACHJZ2_001760"
     CDS             complement(37874..38095)
                     /locus_tag="ACHJZ2_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4567886.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(38194..38409)
                     /locus_tag="ACHJZ2_001761"
     CDS             complement(38194..38409)
                     /locus_tag="ACHJZ2_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4567887.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQPFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(38486..38677)
                     /locus_tag="ACHJZ2_001762"
     CDS             complement(38486..38677)
                     /locus_tag="ACHJZ2_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4567888.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            complement(38650..38832)
                     /locus_tag="ACHJZ2_001763"
     CDS             complement(38650..38832)
                     /locus_tag="ACHJZ2_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO4567889.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            complement(38829..39509)
                     /locus_tag="ACHJZ2_001764"
     CDS             complement(38829..39509)
                     /locus_tag="ACHJZ2_001764"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO4567890.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(39506..40177)
                     /gene="bet"
                     /locus_tag="ACHJZ2_001765"
                     /pseudo
     CDS             complement(39506..40177)
                     /gene="bet"
                     /locus_tag="ACHJZ2_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            40207..40389
                     /locus_tag="ACHJZ2_001766"
     CDS             40207..40389
                     /locus_tag="ACHJZ2_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4567891.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            40386..40556
                     /locus_tag="ACHJZ2_001767"
     CDS             40386..40556
                     /locus_tag="ACHJZ2_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO4567892.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEKDDG"
     gene            40546..>40673
                     /locus_tag="ACHJZ2_001768"
     CDS             40546..>40673
                     /locus_tag="ACHJZ2_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4567893.1"
                     /translation="MMAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERR"
BASE COUNT         9740 a         9919 c        10566 g        10448 t
ORIGIN      
        1 ttttgatgaa atggcaacga tagaggcgag caaataattt tgtattgggg catgcgtgtg
       61 accctttcta acggggttca ctctcggagt cttcatgcga tgagcaagga gtcatgatgt
      121 tagatatagt cgaactgtcg cgcttacagt ttgccttgac cgcgatgtac cacttccttt
      181 ttgtgccact gacgctcggt atggcgttcc tgctggccat tatggaaacg gtctacgtcc
      241 tttccggcaa acagatttat aaagatatga ccaagttctg gggcaagttg tttggtatca
      301 acttcgctct gggtgtggct accggtctga ccatggagtt ccagttcggg actaactggt
      361 cttactattc ccactatgta ggggatatct tcggtgcgcc gctggcaatc gaaggtctga
      421 tggccttctt cctcgaatcc acctttgtag gtctgttctt cttcggttgg gatcgtctgg
      481 gtaaagttca gcatatgtgt gtcacctggc tggtggcgct cggttcaaac ctgtccgcac
      541 tgtggattct ggttgcgaac ggctggatgc aaaacccaat cgcgtccgat ttcaactttg
      601 aaactatgcg tatggagatg gtgagcttct ccgagctggt gcttaacccg gttgctcagg
      661 tgaaattcgt tcacactgta gcgtctggtt atgtgactgg cgcgatgttc atcctcggta
      721 tcagcgcatg gtatatgctg aaaggtcgtg acttcgcctt cgctaaacgc tcctttgcta
      781 tcgctgccag cttcggtatg gctgccgttc tgtctgttat tgttctgggt gatgaatccg
      841 gctacgaaat gggcgacgtg cagaaaacca aactggctgc tattgaagct gagtgggaaa
      901 cgcaacctgc gcctgctgcc tttactctgt tcggcattcc tgatcaggaa gaggagacga
      961 acaaatttgc gatccagatc ccttacgcgc tgggcataat tgcaacgcgt tccgtggata
     1021 ctccagttat cggcctgaaa gaactgatgg tgcagcatga agaacgcatt cgtaacggga
     1081 tgaaggcgta ctctctgctc gaacaactgc gttctggttc taccgaccag gcggttcgtg
     1141 accagttcaa tagcatgaag aaggacctcg gttacggtct gctgctgaaa cgctatacgc
     1201 caaacgtggc tgatgcgact gaagcgcaga ttcaacaggc aaccaaagac tccattccgc
     1261 gtgtagcgcc gctgtacttc gcgttccgta tcatggtggc gtgtggcttc ctgctgctgg
     1321 caatcatcgc gctctctttc tggagtgtca tccgcaaccg cattggcgag aaaaaatggc
     1381 tcctgcgcgc cgcgctgtac ggtattccgc tgccgtggat tgctgtagaa gcgggctggt
     1441 tcgtggctga atatggccgc caaccgtggg ctatcggtga agtgctgccg acagctgtgg
     1501 cgaactcgtc actgaccgca ggcgatctca tcttctcaat ggtgctgatt tgcggcctgt
     1561 ataccctgtt cctggtggca gaattgttct taatgttcaa gtttgcacgc ctcggcccaa
     1621 gcagcctgaa aaccggtcgc tatcactttg agcagtcttc cacgactact cagccggcac
     1681 gctaagacag gagtcgtcaa atgatcgatt atgaagtatt gcgttttatc tggtggctgc
     1741 tggttggcgt tctgctgatt ggttttgcag tcactgatgg tttcgacatg ggggtgggca
     1801 tgctcacccg tttcctcggt cgtaacgaca ccgagcgtcg aattatgatt aactccatcg
     1861 caccacactg ggacggtaac caggtttggc tgatcaccgc aggcggcgca ctgtttgctg
     1921 cctggccgat ggtctatgcc gctgcgttct ccggcttcta tgtggcgatg atcctcgtgc
     1981 tggcgtcttt gttcttccgt ccggtcggtt ttgactaccg ctccaagatt gaagaaaccc
     2041 gctggcgtaa catgtgggac tggggcatct tcattggtag cttcgttccg ccgttggtga
     2101 ttggtgtagc gttcggtaac ctgttgcagg gcgtaccgtt caacgttgat gaatatctgc
     2161 gtctgtacta caccggtaac ttcttccagc tgcttaaccc gttcggcctg ctggcaggcg
     2221 tggtgagcgt agggatgatc attactcagg gcgcaaccta tctgcaaatg cgtaccgtgg
     2281 gcgaactgca cctgcgtacc cgtgcaacgg ctcaggtggc ggcactggtg acactggtct
     2341 gtttcgcact ggctggcgta tgggtgatgt acggtatcga tggttatgtc gtgaaatcga
     2401 caatggacca ttacgcagcc tctaacccgc tgaataaaga agtggttcgt gaagctggcg
     2461 catggctggt taacttcaac aacacgccaa ttctgtgggc tattccggca ctgggtgtgg
     2521 ttctgccgct gctgaccatc ctgactgcac gtatggataa agccgcgtgg gcgtttgtgt
     2581 tctcctccct gacgctggcc tgcatcatcc tgactgccgg tatcgcaatg ttcccgtttg
     2641 tgatgccgtc cagcaccatg atgaacgcaa gtctgacaat gtgggatgca acttccagcc
     2701 agctgacact taacgtcatg acctgggttg cggtggttct ggtaccgatc attctgctct
     2761 acaccgcctg gtgttactgg aaaatgttcg gtcgtatcac caaagaagat attgaacgta
     2821 acacccactc tctgtactaa gtaaggagct aaaaatgtgg tatttcgcat ggattctggg
     2881 aacgcttctt gcctgttcgt ttggggtaat caccgcgctg gcgcttgagc acgtcgaatc
     2941 aggcaaagcc ggtcaagaag acatctgatg agtaagatta tcgcaacctt gtatgcggta
     3001 atggacaagc gccccctgcg ggcgctttcc ttcgtgatgg cgcttctgtt agcaggatgt
     3061 atgttttggg acccatctcg tttcgccgcg aagaccagtg aactggaaat ctggcatggt
     3121 ttattgctga tgtgggccgt ctgtgctggt gtgattcacg gcgtgggctt tcgtccgcag
     3181 aaggttcttt ggcaagggat tttttgccca ttgcttgccg atattgttct cattgtcggg
     3241 ctgattttct tcttctttta aatcagaatt ctctttaaaa aattatgggc cgtgccaggc
     3301 ccataaattt ttacgctccc ttaacttgcc ctcattccca aacctcaatc gcgcgcgtat
     3361 agtagcagcg tttaaaagct ctaacttttg ttgcattacc gggatgtaaa gtgaatacaa
     3421 cgctgtttcg atggccggtt cgcgtctact atgaagatac cgatgccggt ggtgtggtgt
     3481 accacgccag ttacgtcgct ttttatgaaa gagcacgcac agagatgctg cgtcatcatc
     3541 acttcagtca acaggcgctg atggctgaac gcgttgcctt tgtggtacgt aaaatgacgg
     3601 tggaatatta cgcacctgcg cggctcgacg atatgctcga aatacagact gaaataacat
     3661 caatgcgtgg cacctctttg gttttcacgc aacgtattgt caacgccgag aatactttgc
     3721 tgaatgaagc agaggttctg gttgtttgcg ttgacccact caaaatgaag cctcgtgcgc
     3781 ttcccaagtc tattgtcgcg gagtttaagc agtgactgac atgaatatcc ttgatttgtt
     3841 cctgaaggct agccttctgg ttaaacttat catgttgatt ttgattggtt tttcaatcgc
     3901 atcctgggcc attattatcc agcggacccg tattcttaac gcagcggcgc gcgaagccga
     3961 agcgtttgaa gataaattct ggtctggaat cgaactctct cgcctctatc aagagagcca
     4021 ggggaaacgg gataatctga ctggttcgga acaaatcttt tacagcgggt tcaaagaatt
     4081 tgtgcgcctg catcgtgcca atagccatgc gccggaagcc gtagtggaag gggcgtcgcg
     4141 tgcgatgcgt atttccatga accgcgaact tgaaaatctg gaaacgcaca ttccgttcct
     4201 cggtacggtt ggctccatca gcccgtatat tggtctgttt ggtacggtct gggggatcat
     4261 gcacgccttt atcgccctcg gggcggtaaa acaagcaaca ctgcaaatgg ttgcgcccgg
     4321 tatcgcagaa gcgttgattg cgacggcaat tggtctgttc gccgctattc cggcggtaat
     4381 ggcttataac cgtctcaacc aacgtgtaaa caaactggaa ctgaattacg acaactttat
     4441 ggaagagttt accgcgattc tgcaccgcca ggcgtttacc gttagcgaga gcaacaaggg
     4501 gtaagccatg gccagagcgc gtggacgagg tcgtcgcgat ctcaagtccg aaatcaacat
     4561 tgtaccgttg ctggacgtac tgctggtgct gttgctgatc tttatggcga cagcgcccat
     4621 catcacccag agcgtggagg tcgatttgcc agacgctact gaatcacagg cggtgagcag
     4681 taacgataat ccgccagtga ttgttgaagt gtctggtatt ggtcagtaca ccgtggtggt
     4741 tgagaaagat cgcctggagc gtttaccacc agagcaggtg gtggcggaag tgtccagccg
     4801 tttcaaggcc aacccgaaaa cggtctttct gatcggtggc gcaaaagatg tgccttacga
     4861 tgaaataatt aaagcactga acttgttaca tagtgcgggt gtgaaatcgg ttggtttaat
     4921 gacgcagcct atctaaacat ctgcgtttcc cttgcttgaa agagagcggg taacaggcga
     4981 acagtttttg gaaaccgaga gtgtcaaagg caaccgaaca aaacgacaag ctcaagcggg
     5041 cgataattat ttcagcagtg ctgcatgtca tcttatttgc ggcgctgatc tggagttcgt
     5101 tcgatgagaa tatagaagct tcagctggag gcggcggtgg ttcgtccatc gacgctgtca
     5161 tggttgattc aggtgcggta gttgagcagt acaaacgcat gcaaagccag gaatcaagcg
     5221 cgaagcgttc tgatgagcag cgcaagatga aggaacagca ggctgctgaa gaactgcgtg
     5281 agaaacaagc ggctgaacag gaacgcctga agcaacttga gaaagagcgg ttagctgctc
     5341 aggaacagaa aaagcaggct gaagaagccg caaaacaggc cgagttaaag cagaagcaag
     5401 cggaagaggc ggcagcgaaa gcggcggcag atgctaaagc gaaggccgaa gcggatgata
     5461 aagctgcgga agaagcagcg aagaaagcgg ctgcagacgc gaagaaaaaa gcagaagcag
     5521 aagccgccaa agccgcagcc gaagcgcaga aaaaagccga ggcagcagct gcggcgctga
     5581 agaagaaagc ggaagcggca gaagcagctg cagctgaagc aagaaagaaa gcggcagcag
     5641 agaaagctgc agccgacaaa aaagcagcag aaaaagcggc tgctgaaaag gcagcagctg
     5701 ataagaaagc agcggcagaa aaagccgccg cagacaaaaa agcggcagct gcaaaagcag
     5761 cagctgaaaa agccgctgca gcaaaagctg ccgcggaggc agatgatatt ttcggtgagc
     5821 taagctctgg taagaatgca ccgaaaacgg ggggaggggc gaaagggaac aatgcttcgc
     5881 ctgccgggag tggtaatact aaaaacaatg gcgcatcagg ggccgatatc aataactatg
     5941 ccgggcagat taaatctgct atcgaaagta agttctatga cgcatcgtcc tatgcaggca
     6001 aaacctgtac gctgcgcata aaactggcac ccgatggtat gttactggat atcaaacctg
     6061 aaggtggcga tcccgcactt tgtcaggctg cgttggcagc agctaaactt gcgaagatcc
     6121 cgaaaccacc aagccaggca gtatatgaag tgttcaaaaa cgcgccattg gacttcaaac
     6181 cgtaatcgcg atgttgactg ttccgacggt caacatcagg caccggttgc cacggggttc
     6241 tggtagtttt gtgtatttta gtttgttaac attctgctaa attatcgtgg gccatcggtc
     6301 cagataaggg agatatgatg aagcaggcat tacgagtagc atttggtttt ctcatactgt
     6361 gggcatcagt tctgcatgct gaagtccgca ttgtgatcga cagcggtgta gattccggtc
     6421 gtcctattgg agttgttcct ttccagtggg cgggccctgg tgcggcacct gaagatattg
     6481 gcggcatcgt tgctgccgac ttgcgtaaca gcggtaaatt taatccgtta gatcgcgctc
     6541 gtctgccaca gcagccgggt agtgcgcagg aagtacaacc agctgcatgg tccgcgctgg
     6601 gtattgacgc tgtagttgtc ggtcaggtca ctccgaatcc ggatggttct tacaatgttg
     6661 cttatcaact tgttgacact ggcggcgcac cgggtactgt acttgctcag aactcgtaca
     6721 aagtgaacaa gcagtggctg cgttatgctg gtcataccgc cagtgatgaa gtgtttgaaa
     6781 aactgaccgg tattaaaggt gcgttccgta cccgtattgc ctacgttgtt cagaccaacg
     6841 gcggtcagtt cccgtatgaa ctgcgcgtat ctgactatga cggttacaac cagtttgtcg
     6901 ttcaccgttc accacagccg ctgatgtccc cggcgtggtc accagacggt tctaaactgg
     6961 cttatgtgac cttcgaaagc ggtcgttccg cgctggttat acaaacgctg gcaaacggcg
     7021 ctgtacgtca ggtggcttca ttcccgcgtc acaacggtgc gcctgcattc tcgccagacg
     7081 gcagcaaact ggcattcgcc ttgtcgaaaa ccggtagcct gaacctgtac gtaatggatt
     7141 tggcttctgg tcagatccgc caggtgactg atggtcgcag taacaatacc gaaccgacct
     7201 ggttcccgga cagccagaac ctggcattta cttctgacca ggccggtcgt ccacaggttt
     7261 ataaagtgaa tatcaacggc ggtgcgccac aacgtattac ctgggaaggt tcgcagaacc
     7321 aggatgcgga tgtcagcagc gacggtaaat ttatggtaat ggtcagctcc aatggtgggc
     7381 agcagcacat tgccaaacaa gatctggcaa cgggaggcgt acaagttctg tcgtccacgt
     7441 tcctggatga aacgccaagt ctggcaccta acggcactat ggtaatctac agctcttctc
     7501 aggggatggg atccgtgctg aatttggttt ctacagatgg gcgtttcaaa gcgcgtcttc
     7561 cggcaactga tggacaggtc aaattccctg cctggtcgcc gtatctgtga taataattaa
     7621 ttgaatagta aaggaatcat tgaaatgcaa ctgaacaaag tgctgaaagg gctgatgatt
     7681 gctctgcctg ttatggcaat tgcggcatgt tcttccaaca agaacgccag caatgacggc
     7741 agcgaaggca tgctgggtgc cggcactggt atggatgcga acggcggcaa cggcaacatg
     7801 tcttccgaag agcaggctcg tctgcaaatg caacagctgc agcagaacaa catcgtttac
     7861 ttcgatctgg acaagtacga tatccgttct gacttcgctc aaatgctgga tgcacatgca
     7921 aacttcctgc gtagcaaccc gtcttacaaa gtcaccgtag aaggtcacgc ggacgaacgt
     7981 ggtactccgg aatacaacat ctccctgggt gaacgtcgtg cgaacgccgt taagatgtac
     8041 ctgcagggta aaggcgtttc tgcagaccag atctccatcg tttcttacgg taaagaaaaa
     8101 cctgcagtac tgggtcatga cgaagcggca tactccaaaa accgtcgtgc ggtactggtt
     8161 tactaagaga attgcatgag cagtaacttc agacatcaac tattgagtct gtcgttactg
     8221 gttggtatag cggccccctg ggccgctttt gctcaggcac caatcagtag tgtcggctca
     8281 ggctcggtcg aagaccgcgt cattcaactt gagcgtattt ctaatgctca cagccagctt
     8341 ttaacccaac tccagcaaca actctctgat aatcaatccg atattgattc cctgcgtggt
     8401 cagattcagg aaaatcagta tcaactgaat caggtcgtgg agcggcaaaa gcagatcctg
     8461 ttgcagatcg acagcctcag cagcggtggt gcagcggcgc aatcaaccag cggtgatcaa
     8521 agcggtgcgg cggcatcaac gacgccgaca gctgatgctg gtactgcgaa tgctggcgcg
     8581 ccggtgaaaa gcggtgatgc aaacacggat tacaatgcag ctattgcgct ggtgcaggat
     8641 aaatcccgcc aggatgacgc aatggtggca tttcagaatt tcatcaaaaa ttaccctgat
     8701 tcaacttacc tgccaaacgc caattattgg ctgggtcagt taaactacaa caagggtaaa
     8761 aaagatgatg cggcgtacta ttttgcttcg gtagtgaaaa actatccgaa gtcaccaaag
     8821 gctgcagatg cgatgtttaa agtcggcgtc atcatgcagg acaaaggtga caccgcaaaa
     8881 gcgaaagccg tataccagca ggttatcagt aaataccctg gtactgatgg cgctaaacag
     8941 gcacaaaaac gtctgaacgc gatgtaatgc ataacacacg accagaagtc gcattatttc
     9001 tggtcgtgtc gtgcgaatca taagcagttg agtgatctac atcgaaattt ttgttgcgct
     9061 caagtctgaa atcagtaata tatgccgccg ttgccacggg atatcaaaca aaccgaaagc
     9121 aacgaaaaag tgggtcgtta gctcagttgg tagagcagtt gacttttaat caattggtcg
     9181 caggttcgaa tcctgcacga cccaccacta acctagttag ttgtagtatc cagcgtaata
     9241 tcgggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga
     9301 tcccgtcatc acccaccacc gggtcgttag ctcagttggt agagcagttg acttttaatc
     9361 aattggtcgc aggttcgaat cctgcacgac ccaccactaa ttacggtggg ttcggtggaa
     9421 gtagtttgta gtatccagcg cagtatcggg tgattagctc agctgggaga gcacctccct
     9481 tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccactcgggt cgttagctca
     9541 gttggtagag cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg cacgacccac
     9601 caattttaac atcaaactca gatgttaagc gtgaaggata acgttgcgtc agcaacggcc
     9661 cgtagggcaa gcgaagcgag tcatcctgca cgacccacca attttaaaga ttggttccga
     9721 gtagaaatct ttcaggtaac acccgtatgg gtcgttagct cagttggtag agcagttgac
     9781 ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc
     9841 taaaggcgct tttttgctat tcaggcatct ctcaatttca attcttgacc ctgatgacat
     9901 actccgcgct tgctgcgtaa acaactttac gtcggaggaa taagacgcaa agtgtggcat
     9961 tcggcatctc atacgtaatt cgagtgactt ttccccacca ttcgactatc ttgtttagca
    10021 tataaaacaa attacaccga taacagcgaa tatcacgcta atgtcggttt taacgttaag
    10081 cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc gatttatcct
    10141 ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg cgagaagata
    10201 aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac cgatcccgaa
    10261 attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga aatggcgcgc
    10321 ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt tatgggagaa
    10381 accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc cgacacttca ggctgagtgt
    10441 tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc ccatcccgat
    10501 cgtactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgggc agattgggtg
    10561 gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagtttggg tgaaaaaatc
    10621 atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aaaagacggg cgcagacatt
    10681 ttatgctggc agggtgcctg cattgtgcat gatgagttta agactcaggc gttaacccgc
    10741 ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc acaagctatt
    10801 gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc gaaaacattg
    10861 ccacatcaga ggcttattgt ggcaaccgat cggggtattt tctacaaaat gcagcaggcg
    10921 gtgccagata aagagttact ggaagcacca accgcgggtg agggcgcaac ctgccgcagc
    10981 tgcgcgcatt gtccgtggat ggccatgaat ggcctccagg ccatcgctga ggcattagaa
    11041 caggaaggaa gcaattacga ggttcatgtt gatgaaaggc tgcgagagag ggcgctggtg
    11101 ccgctcaatc gtatgctgga ttttgcggct acactacgtg gataacgaat aataaggcgt
    11161 aacgttacgc tttgggggaa agatggattt ttttagtgtg cagaatatcc tggtacatat
    11221 accaatagga gcaggcggtt atgatctctc atggatcgaa gcggtaggca caatcgccgg
    11281 gttgctgtgt attggccttg ccagtctgga gaagatcagc aactacttct ttggcctgat
    11341 caacgtcacc ttgtttggca ttattttctt tcagattcag ctgtatgcca gcctgctatt
    11401 acaggtgttt ttctttgccg cgaatattta cggctggtat gcgtggtcgc gacaaaccag
    11461 tcagaacgag gcggagttaa aaattcgctg gttgccattg ccgaaggcac tcagctggtt
    11521 ggcggtttgc gttgtttcga ttggtctgat gacggtattt atcaatccgg tgtttgcatt
    11581 tttgacccgc gtggcagtca tcatcatgca agcattagga ttacaggttg cgatgcctga
    11641 actacaaccg gacgctttcc cgttctggga ttcttgcatg atggtgttat ctatcgtggc
    11701 aatgattctg atgactcgta agtatgtgga aaactggctg ttgtgggtga ttattaacgt
    11761 gattagcgtc gttatttttg cacttcaggg cgtttacgcc atgtctctgg agtacatcat
    11821 cctgaccttt attgcgctca acggcagccg gatgtggatc aatagcgcac gcgaaagagg
    11881 ctcacgcgcg ctgtcccatt aatggtgatg atgtgaatgg ccagacaccc cttcattcag
    11941 gtggcagtct ggcccatgac aaggttgata ttccatctga atggtggcgt gctcaatctg
    12001 atagtgatcc atcaggtagt gttggatctg atccaacaag gcatcgtgat cgtgtggcgg
    12061 gatcacctgc acatgcaacg tcatcaccgg cttctcgcct accatccata catgcacatg
    12121 gtgcacattg cggacttccg ggatttcccg gcacatacga cgcttcagtt cagcgatatc
    12181 cagcgatacc ggtgcaccct caagtaattc attcacgcta tctttcaaca gtcgccacgc
    12241 gctacgcaga accagaagcg acaccagtat cgagagaatg gggtcagcag gtgtccagcc
    12301 ggtccagata ataatcaacg cggcaataat agccccaacc gaacccagca ggtccccgag
    12361 cacatgcagt gccgctgctc gtacgttgag gtttttctct tcgctgccgt gatgaagtaa
    12421 ccagaaagaa agtatatttg ccagcaaccc agccacggca attgccatca tcatgccgcc
    12481 ctcgaccgga cgcggcgtgc ggaagcgttc tatcgcctcc cagacaatca aaatggtaat
    12541 taccacgagg gcgatggcat tcacaaacgc cgcgagcgtg gtcaatctta gccaaccgaa
    12601 agtgtggcga atggtgggag gacgacgtga aaattgcacg gcgagtaggg cgaaaagcag
    12661 ggcggcagta tcggtcaaca tatgacccgc atcggccagc aaagccagag aaccggaaag
    12721 aaaaccacca atgacttcta ccagcataaa cccggcagtc acgccgaaag catacaacaa
    12781 gcgacgagca ttattatctt ctggcaggtg tgaggacgtg tgtgagtgtg agtgcgccat
    12841 gacgagtcat cccttaatta ttctcactta actttatgac atcatactgc ttttagaagg
    12901 gaaaaattaa aagggagaga ctccgctctc ccattattgg ctattttgca gggttactgc
    12961 gtggtaccgt cggttttggt atcgacatca ttattgatgc catcaccggt ttgtaccttt
    13021 ttattgatat ccgggcagcg accatctttg cacatggtgt tcttgtgctc ttcatctttg
    13081 gtcatgccgt cattgttcat tgaagaacca tccggatgca gcatagtgcc gccagaaccg
    13141 gtatttaccc cgttattgtc gacgttattt ggcgcgacat tttcacgggc gtcaggggct
    13201 acctggcccg catcagctgc ggcgtttgcc tggccgttat tggtttgcgc tccgctatcg
    13261 gcagccagtg cggcaccgct ggcaaggctt agagtggcag tcagaaatag tgtggccagt
    13321 tttgtcattt tcataggatg ctcctgttat ggtcgttatg tcggataacc tcttccaaca
    13381 gtgcatttgc aggtgaatat aaggcattgg tttaagattt cagccaggca aagaaatcca
    13441 gcagagaatc ttgaaataat taacaaacaa aggagttaca gttagaaatt gtaggagaga
    13501 tctcgttttt cgcgacaatc tggcgttttt cttgctaatt ccaggattaa tccgttcata
    13561 gtgtaaaacc ccgtttacac attctgacgg aagatataga ttggaagtat tgcattcact
    13621 aagataagta tggcaacact ggaacagaca tgaattatca gaacgacgat ttacgcatca
    13681 aagaaatcaa agagttactt cctcctgtcg cattgctgga aaaattcccc gctactgaaa
    13741 atgccgcgaa tacggttgcc catgcccgaa aagcgatcca taagatcctg aaaggtaatg
    13801 atgatcgcct gttggtggtg attggcccgt gctcaattca tgatcctgtc gcggctaaag
    13861 agtatgccac tcgcttgctg gcgctgcgtg aagagctgaa agatgagctg gaaatcgtaa
    13921 tgcgcgtcta ttttgaaaag ccgcgtacta cggtgggctg gaaagggctg attaacgatc
    13981 cgcatatgga taacagcttc cagatcaacg acggtctgcg tatagcccgt aaattgctgc
    14041 ttgatattaa cgacagcggt ctgccagcgg cgggtgaatt cctcgatatg atcactcctc
    14101 agtatctcgc tgacctgatg agctggggcg caattggtgc acgtaccacg gaatcgcagg
    14161 tgcaccgcga actggcgtct ggtctttctt gtccggtagg ttttaaaaat ggcacagacg
    14221 gtacgattaa agtggctatc gatgccatta atgccgccgg tgcgccgcac tgcttcctgt
    14281 ccgtaactaa atgggggcat tcggcgattg tgaataccag cggtaacggc gattgccata
    14341 tcattctgcg cggcggtaaa gagcctaact acagcgcgaa gcacgttgct gaagtgaaag
    14401 aagggctgaa caaagcaggt ctgccagctc aggtgatgat cgatttcagc catgctaatt
    14461 cgtccaaaca attcaaaaag cagatggatg tttgtgctga cgtttgccag cagattgccg
    14521 gtggcgaaaa ggccattatc ggtgtgatgg tggaaagtca tctggtggaa ggcaatcaga
    14581 gcctggagag cggggagccg ctggcctatg gtaagagcat caccgatgcc tgcattggct
    14641 gggaagatac cgatgctctg ttacgtcaac tggcgaacgc agtgaaagcg cgtcgcgggt
    14701 aaagtttaat tgtcggatgc gccgtcagag tggcgtatcc gatgaatcac cgtagccctg
    14761 acaagacgcg cagcgtcgca tcaggcaatg tgctccattg ttagcgacga caaacaaaaa
    14821 agccgactca cttgcagtcg gctttctcat ttttaaacga atgacgttta cttcgcttta
    14881 ccctggttcg caacggctgc tgctttcgcc gcgatctcgt cagcattacc cagataatag
    14941 cgtttcagcg gtttgaagtt ctcgtcaaac tcataaacca gcggcacgcc ggtcgggata
    15001 ttcagctcaa gaatctcttc ttcgctcatg ttatcgagat atttcaccag cgcacgtaac
    15061 gagttaccgt gtgcagcgat gattacgcgc tcaccgctct tcatacgcgg cagaatggtt
    15121 tcattccagt aagggatcac gcggtcaatg gtcagcgcca ggctttccgt cagcggcagt
    15181 tctttctcgc tcagtttcgc gtaacgcgga tcgtgacccg gataacgctc atcatcttta
    15241 gtcagttccg gcggagtcac tgcaaaacca cgacgccact gtttcacctg ctcgtcgcca
    15301 tatttttcag cagtttccgc tttgttcaga ccctgcaacg caccgtagtg acgttcgttc
    15361 agtttccagg atttctcaac gggcagccat gcctgatcca gttcgtccag cacattccac
    15421 agggtatgga tagcgcgttt cagcacagag gtgtaagcaa agtcaaagct gtaaccttcc
    15481 tctttcagca gcttacctgc tgcttttgct tcgcttacgc ctttctcaga cagatccacg
    15541 tcgtaccaac cggtgaaacg gttttctttg ttccactgac tttcgccatg acgaaccaga
    15601 accagcttag ttacagccat atacttactc ctcaaatcat cttttaatga taataattct
    15661 cattatattg ccgtgacgaa gcaacagcaa tgcttacgca taaccatagc gaaaatagtg
    15721 gcgcagtgta aggttgttgt gaatattgag ttgcaaatat gtcggtgttt gctggtgatt
    15781 tgaacaatat gagataaagc cctcatgacg agggcttaac attactcagc aataaactga
    15841 tattccgtca ggctggaata ctcttcacca ggatgcagga aacagtccgg ttgcggccat
    15901 tcagggtggt tcgggctgtc cggcagaaac tcgctttcca acgccagccc ttgccagtcg
    15961 gcgtaaggtt cggttccccg cgacggtgtg ccgccgaggt agttgccgga gtagaattgc
    16021 agagccggag cggtggtgta gaccttcagc tgcaattttt catctgctga ccagacatgc
    16081 gccgccactt tcttgccatc gcctttggcc tgtaacaaga atgcgtgatc gtaacctttc
    16141 actttgcgct gatcgtcgtc ggcaagaaac tcactggcga tgattttggc gctgcggaaa
    16201 tcaaaagacg ttccggcgac agatttcagg ccgtcgtgcg gaataccgcc ttcatcaacc
    16261 ggcagatatt cgtccgccag aatctgcaac ttgtgattgc gcacgtcaga ctgcttgccg
    16321 tcaagattga aatagacgtg attagtcata ttcaccgggc aaggtttatc aactgtggcg
    16381 cgataagtaa tggagatacg gttatcgtcg gtcagacgat attgcaccgt cgcgccgaga
    16441 ttacccggga agccctgatc gccatcatct gaactcaggg caaacagcac ctgacgatcg
    16501 ttctggttca caatctgcca gcgacgtttg tcgaaccctt ccgggccgcc gtgcagctgg
    16561 ttaacgccct gacttggcga aagcgtcacg gtttcaccgt caaaggtata acgactattg
    16621 gcgatgcggt tggcataacg accaatagag gcacccagaa acgcggcctg atcctgatag
    16681 cattccgggc tggcacagcc gagcagcgcc tcgcggacgc tgccatcgga aagcggaata
    16741 cgggctgaaa gtaaagtcgc accccagtcc atcagcgtga ctaccatccc tgcgttgtta
    16801 cgcaaagtta acagtcggta cggctgacca tcgggtgcca gtgcgggagt ttcgttcagc
    16861 actgtcctgc tccttgtgat ggtttacaaa cgtaaaaagt ctctttaata cctgtttttg
    16921 cttcatattg ttctgcgaca gcttgctgta cggcaggcac cagctcttcc gggatcagcg
    16981 cgacgataca gccgccaaat ccgccgccgg tcatgcgtac gccacctttg tcgccaatca
    17041 cagctttgac gatttctacc agagtgtcaa tttgcggcac ggtgatttcg aaatcatcgc
    17101 gcatagaggc atgagactcc gccatcaact cgcccatacg tttcaggtcg ccttgctcca
    17161 gcgcgctggc agcttcaacg gtgcgggcgt tttcagtcag gatatgacgc acgcgtttcg
    17221 ccacgattgg gtccagctca tgtgcaacag cgttgaactc ttcaatggtg acatcgcgca
    17281 gagctggctg ctggaagaaa cgcgcaccgg tttcgcactg ttcacgacgg gtgttgtatt
    17341 cgctgccaac cagggtacgt ttgaagttac tgttgatgat gacgacagcc acacctttcg
    17401 gcatggaaac tgctttggtc cccagtgagc gacagtcaat cagcaaggca tgatctttct
    17461 tgccgagtgc ggaaattagc tgatccatga tcccgcagtt acagccaaca aactggtttt
    17521 ctgcttcctg accgttaagc gcgatttgtg cgccgtccag cggcagatga taaagctgct
    17581 gcaatacggt tccgaccgcg acttccagtg aagcggaaga acttaacccg gcaccctgcg
    17641 gcacattgcc gctgatcacc atgtccacac cgccgaagct gttgttacgc agttgcagat
    17701 gtttcaccac gccacgaacg tagttcgccc attgatagtt ttcatgcgcg acaatgggcg
    17761 catcaaggga aaactcatca agctgatttt cataatcggc tgccattacg cgaactttac
    17821 ggtcatcgcg tggcgcacag ctgatcacgg tttgataatc aatcgcgcag ggcagaacga
    17881 aaccgtcgtt gtagtcggtg tgttcaccaa tcaaattcac gcggccaggc gcctgaatgg
    17941 tatgagtggc agggtagcca aatgcgttgg caaacagaga ttgtgttttt tctttcagac
    18001 tcatttctta cactccggat tcgcgaaaat ggatatcgct gactgcgcgc aaacgctctg
    18061 ctgcctgttc tgcggtcagg tctcgctggg tttctgccag catttcataa ccaaccataa
    18121 atttacgtac ggtggcggag cgcaacagag gcggataaaa gtgcgcgtgc agctgccagt
    18181 gttgattctc ttcgccatta aacggtgcgc cgtgccagcc catagagtag gggaaggagc
    18241 actggaagag gttgtcataa cgactggtca gctttttcaa cgccagtgcc aaatcgctgc
    18301 gctgggcgtc ggtcaaatcg gtgatccgca aaacgtgggc tttgggcaga agtagcgttt
    18361 cgaacggcca ggcagcccag taaggcacaa ctgctaacca gtgttcggtt tcgacaacgg
    18421 tacggctacc gtctgccagc tcgcgctgaa cataatccac cagcattggc gatttctgct
    18481 cggcaaaata ttctttttgc aggcggtctt cgcgctcagc ttcgttaggc aggaagctat
    18541 ttgcccaaat ctgtccgtgc ggatgcgggt tagagcagcc catcgccgca cctttgtttt
    18601 caaagacctg cacccacggg tatgttttcc ccagttctgc ggtttgctcc tgccaggttt
    18661 tgacgatttc cgtcaatgcc gcaacgctca gttctggcag cgttttactg tgatccggtg
    18721 aaaagcagat cacccggctg gtaccgcgcg cgctctggca acgcatcaaa ggatcgttgc
    18781 tttctggcgc atctggcgtg tcagacatca aggccgcaaa gtcattagtg aaaacgtaag
    18841 tcccggtgta atcggggttt ttatcgcctg tcacccgcac attacctgcg cagaggaagc
    18901 aatctggatc gtgcgcaggt aacacctgtt tggctggcgt ttcctgcgcc ccctgccagg
    18961 ggcgcttagc gcggtgcggt gaaaccagaa tccattgccc ggtgagctgg ttgtagcggc
    19021 gatgtggatg atcaacggga ttaaattgcg tcatggtcgt tccttaatcg ggatatccct
    19081 gtggatggcg tgactgccag tgccaggtgt cctgcgccat ttcatcgagt gtgcgcgtta
    19141 cgcgccagtt cagttcacgg tcggctttgc tggcgtccgc ccagtaggcc ggaaggtcgc
    19201 cctcgcgacg cggtgcaaaa tgatagttaa ccggtttgcc gcaggctttg ctgaaggcat
    19261 taaccacgtc cagcacgctg ctgcctacgc cagcaccgag gttgtagatg tgtacgcctg
    19321 gcttgttcgc cagtttttcc atcgccacga cgtgaccgtc cgccagatcc attacgtgga
    19381 tgtaatcgcg tacgccagta ccgtcttcgg tcggataatc gttaccaaaa atcgccagcg
    19441 agtcgcgacg gcctacagca acctgggcga tgtatggcat aaggttattc ggaatgcctt
    19501 gcggatcttc gcccatatcg cccgacggat gtgcgccaac cgggttgaag tagcgcagca
    19561 gggcaatgct ccagtccggc tgggcttttt gcagatcggt gaggatctgt tccaccatca
    19621 gcttgctttt gccgtaaggg ctttgcggtg tgccggtcgg gaagctttca acgtatggaa
    19681 ttttgggctg atcgccataa acggtggcgg aggagctaaa aataaagttt ttgacgttag
    19741 cggcgcgcat ggcgctaatc aggcgcagag taccgttgac attgttgtcg taatattcca
    19801 gcggtttttg taccgattcg ccaacggctt tcagcccggc gaagtggatc acggtgtcga
    19861 tagcgtgatc gtgcaggatc tcggtcatca acgcttcgtt acggatatcg ccttcaacaa
    19921 acgtcggatg tttgccgcct aaacgctcga taacaggcag tacgctgcgc ttactgttac
    19981 agaggttatc aagaatgatg acatcatgac cgttttgcag taattgcaca caggtatgac
    20041 ttccaatgta accgctacca ccagtgacca gaactctcat aattcgctcc attaggctta
    20101 tggtatgaaa taaccatagc ataacaaaga tgcgaaaagt gtgacatgga ataaattagt
    20161 ggaatcgttt acacaagaat ttagccgttt tttatgcgcg attaagtgat tataaaacag
    20221 agggattgtg aatgattgcg ctttttatct gaaaaaaaga cgcagtttca tgcctggatg
    20281 cgtctgaccg tgtgccggat aaagcgttta cgccgcatcc ggcacagtca gcattaccga
    20341 cttagtttat ttttgtcagc gcataatgat agaggccacc gtccggcacg aactccagac
    20401 gatgggtaat acaagccggc gcatcttcag catggtgcga aacaaacaac aattgcgttt
    20461 caccttcgct aatcagcaca tcaacaaaac ggcgaataag ctggcgattg agcggatcaa
    20521 gcccctgcaa tggttcgtcg agaataagca acgtcggatg tttcaccagt gcgcggacga
    20581 tcagcgccag acgctgctgt ccccaggaaa gactatggaa cggagcgtca gccgtgcgtt
    20641 tatcaatgcc gagaatatcc agccactgct gcaccagttt ttgctggcga tccgaaacgg
    20701 cctgataaat gccaatcgaa tcaaaatagc cagaaagaat cacattacgc acagtagtac
    20761 tgacccggta atccagatgc aaactactgc tgacgtaacc gatatgcttt ttgatatccc
    20821 agatggtttc gccgctgcca cgacgtcgtc cgaaaagcgt caaatcgttg ctgtaacctt
    20881 gcggatgatc gccagtaatc aggcttaata acgtcgattt tcccgcgcca tttggcccga
    20941 caatttgcca gtgttcacct ggattcacct gccagctaag gttattaaga atggggcgat
    21001 cgttataaga aaccacgcca ttattcagca caatgcgcgg ttcgttggcg ggtaaggcgt
    21061 gacgtgctga aggttcatcc ggctccggca gttgcacacc ttcaagctgt tcactatgcg
    21121 ccagttgcgc gacgagtgct tgttggagca gttcctcttt agcgccagtt tccgctaacg
    21181 tgcaatccgc cagcacgcca gcaaactgga caaactccgg gatctcatcg aagcgattga
    21241 gcaccagtac cagagtaata ccggactgat gtaacgaggc gagtcgctca gccagctgct
    21301 gacgtgaggc aacatccagg ccatcgaacg gctcatcaag aatcaacaag tcaggctccg
    21361 acatcagcgc ctgacacagc agggtttttc gcgtctcgcc agtggaaagg tatttaaagc
    21421 gtcggtcgag gaggggggta ataccgaact gctgtgccag ttgcatgcag cgcggtgcat
    21481 cctttacttc atcctgaata atctcagccg tagtgcgtcc ggtgtcatct tcgccagggc
    21541 cgagcatatc ggtgttattc cgctgccatt cgtcgctgac gagcttttgc aattgctcga
    21601 aggagagacg agtgatgtgg gaaaactggc tttgccgttc acctttcaaa agcggaagtt
    21661 cccccgccag cgcgcgggcc agggccgatt tcccgcttcc attcgaaccg acaaacgccc
    21721 aactatcacc cgcgtttaac gttagctgag gcaattgcag cgtttttgtg tcgctaagac
    21781 gaaacgtgcc ttgcaaaatt tgcaacgatg acattttata tcccattttg tgcagcgatt
    21841 actgacaggg atacgtgttt cataacaaat tgtcaacacg cttagcacag tgtggcgata
    21901 atcacgctgt cggcattaaa gtaggccgtg acattctgtc cttgctgaag agaagtcgct
    21961 tcatttaccg gcactgtggc gcacagtgtt tgcccgtccg gtagcgccat taatacttcg
    22021 cactgctctg cgccgcgctc aatatgacta ataatacccg gtaattggtt gtcagcgttt
    22081 tgcgcgaccg cttcgtcctg agtaatgcct acccacggcg cttttagcaa tatcaacact
    22141 tctttgcctt catccagccc cagacgcgcg ccgctttgtg cggtaattgc gactttcagg
    22201 cgtgtttttc catcagccag taagacatca acatgctgtt gaacgtcatc atgatcgcgg
    22261 gcggtgatgg taccgaacca ctggttacgg gcgctggttt ggagtgaaaa acgtgagatc
    22321 gcggccagca ggctgttcag cggcagggcg tcatcgtcac ttaacacatc aaaggctttt
    22381 tgctggattt gcgccagtaa gtcatagagc tgaatcagtc gctgaccata gcgggtcagt
    22441 actgcgccgc cgccaccttt accgcctgtt gcgcgctcga ccagaatatg ctcacttaac
    22501 tgattcatct cgttaatggc atcccaggcg cttttatagc tgataccggc atctttcgct
    22561 ccctggctaa tggaaccgga aagcgcaatg tgttttagta gcgaaatgcg gcgcgggtcg
    22621 gcgaataatt tttgttggag cttaagggtg agaaggattt cggcctgcat aacaatgtcc
    22681 tggcaaaagt cttattgtga cggaaaacga acgccacgca aagctgaccg cacaaaaggg
    22741 gagtgctttt ctgtgcttag cggttagaat agtctcatga ctatatctgg agttgaccat
    22801 gttagagtta ttaaaaagtc tggtattcgc cgtaatcatg gtacctgtcg tgatggccat
    22861 catcctgggt ctgatttacg gtcttggtga agtattcaac atcttttctg gtgttggtaa
    22921 aaaagaccag cccggacaaa atcattgatt ccctgaatgc ccgcttagtc gggcattttc
    22981 tttttctcaa cttcctgctt ttcctgccga tattttttct tatctacctc acaaaggtta
    23041 gcaataactg ctgggaaaat tccgagttag tcgttatatt gtcgcctaca taacgttaca
    23101 ttaaggggtt accaatggct cgtaaatggt tgaacttgtt tgctggggcg gcactctctt
    23161 tcgctgttgc tggcaatgca ctggcagatg aagggaaaat cacggtgttc gccgccgcat
    23221 cactgactaa cgcaatgcag gacattgcta cgcagtataa aaaagagaaa ggcgtggatg
    23281 tggtttcttc tttcgcttcg tcatctactc tcgcccgtca gattgaagcg ggtgcccctg
    23341 cggatctgtt tatttctgcc gatcagaaat ggatggatta tgcggttgat aaaaaagcga
    23401 tcgatacagc tacgcgtcag acactgctcg gcaatagcct ggtcgttgta gcaccgaaag
    23461 ccagcgagca gaaagatttt accatcgaca gcaaaaccaa ctggacttca ctgctgaatg
    23521 gcggtcgcct ggcggttggc gatccggaac atgttcccgc tggcatttat gcaaaagaag
    23581 cactgcaaaa actgggcgca tgggatacgc tctccccgaa actggctccg gcggaagatg
    23641 ttcgtggcgc gctggcgttg gtcgaacgca atgaagcgcc gttgggcatt gtttacggtt
    23701 ctgacgcagt tgctagcaaa ggggtaaaag tggttgccac cttcccggaa gattcacata
    23761 aaaaagtgga atatccggtt gctgttgtgg aagggcataa caatgcgaca gtgaaagcat
    23821 tttatgatta tctgaaggga ccgcaggcag cggaaatctt taaacgttac ggatttacaa
    23881 ccaagtaatg atactgaccg atccagaatg gcaggcagtt ttattaagcc tgaaagtttc
    23941 ttccctggct gtgctgttta gcctgccgtt tgggatcttt tttgcctggt tactggtgcg
    24001 ttgcacgttt ccgggcaaag ctctgctcga cagcgtactg catctaccgc tggtgttacc
    24061 gcccgtggtc gtcggttact tattattagt ttcgatgggg cggcgcggat ttatcggtga
    24121 acgtctgtat gactggtttg gtattacctt cgcctttagc tggcgcggcg cggttctcgc
    24181 tgccgccgtc atgtcgtttc cgctgatggt gcgggcaatt cgtctggcgc tggaaggggt
    24241 tgatgtcaaa ctggaacagg ccgcaagaac actgggggcc gggcgctggc gcgttttctt
    24301 tactatcacg ttaccgctga ccttaccggg aattattgtt ggtacggtac tggcttttgc
    24361 tcgttctctc ggtgaatttg gcgcaaccat cacctttgtg tcgaacattc ctggtgaaac
    24421 gcgaaccatt ccttctgcca tgtataccct gattcagacc cccggcgggg aaagtggcgc
    24481 agcgagactg tgcattattt ctattgcgct ggcgatgatc tccctgttga tttcagaatg
    24541 gctggccaga atcagccgtg aacgggcggg gcgctaatca tgctggaact gaatttttcc
    24601 cagacgttgg gcaaccattg cctgactatt aatgaaacgc tgcccgccaa tggcatcact
    24661 gctatctttg gcgtctccgg tgccggaaaa acttcgctga ttaacgccat cagtggactg
    24721 acgcgcccgc aaaaagggag gattgtcctc aatggtcggg tactaaatga tgccgaaaaa
    24781 ggtatctgcc tgacgccgga aaagcgtcgc gttggctatg tttttcagga tgcgcggctg
    24841 ttcccgcatt acaaagtgcg tggcaatctg cgctacggca tggcgaaaag catggtcgat
    24901 cagttcgata agctggtggc gcttttaggc attgaaccgt tgcttgaccg tttaccaggc
    24961 agcctgtccg gaggcgaaaa acagcgcgta gcgattgggc gggctttgct gacggcacca
    25021 gaattgctgt tgctggatga accgctggcg tcactggata ttccgcgtaa acgcgaactg
    25081 ttgccttatc tgcaacgtct gacgcgggaa atcaacatcc cgatgttgta tgtcagccat
    25141 tcgctggatg agatcctcca tctggcagac agagtgatgg tactggaaaa cggtcaggtg
    25201 aaagcctttg gcgcgctgga agaagtctgg ggtagtagcg tgatgaatcc gtggctgccg
    25261 aaagagcaac aaagtagcat tctgaaagtg acggtacttg agcatcatcc gcattacgcg
    25321 atgaccgcgc tggcgctggg cgatcagcat ttgtgggtca ataagctgga cgaaccgctg
    25381 caagctgcgc tacgtatccg cattcaggct tccgatgttt ctctggtttt acaaccgccg
    25441 cagcaaacca gcattcgtaa cgtattgcgg gcaaaagttg ttaatagtta tgacgacaac
    25501 ggccaggtgg aagtggaact ggaagtcggc ggtaaaacgc tgtgggcgcg tatcagcccg
    25561 tgggccaggg atgaactggc gatcaaacct ggcctgtggc tgtacgcgca aattaaaagt
    25621 gtgtcgataa ccgcctgatt aaatcaggtg gctataaatg aactgggcaa tgctgtcggt
    25681 ggtgttatca ccaatcacaa tgttggcgcg cgcttttacc gcgtcatcgg cgttgcccat
    25741 cgccacgcct gtaccagcgg cctccagcat actgatatca ttaaagttat cgccgaacgc
    25801 cacgacgttt tccatcgacc agccttgcgc ctcaacccat ttcgtcaaac gtttaccttt
    25861 gctgttgccg ccgcgtgcaa tatcaacctg atcgtgccag gaccattcac actccagtcc
    25921 cagttcatgt tcgacatgct taccaaaatg ctgcaattgc ggcaggtcat cgtgcgtgag
    25981 ggcgaacttc catacggcgt taacttgttg cgccgtttca gccagagaag cgacttgtgt
    26041 gaaagtcgga cgctgttccg gcggcagggt ttgcgcccag ttagacgtgc gaatgacatg
    26101 cccggtcggg tgctcataaa ccatctcgtc atcgacatac atcagaccgt gaatgtggtg
    26161 ttcattcagc atctcaatga gttgtagggc tttattaacg ggcattgggt ccgcttccag
    26221 cacggttttt gcatgataat catacaaata ggtgccatta cagcaaatag caggtgtatc
    26281 cagcgccagc gcctgataaa aaggatgaat agcgacgtga tggcgacctg tgacgatgat
    26341 taattgataa cctgcttcgc gagcgcgggc cagggcttct atcgatgaag gtagcagggt
    26401 ctttttcggg gtcaataagg tgccgtctaa gtcgagagca atcacgcgtg tggtcatggc
    26461 gtattccaga ttaaggttaa gaattttctt ctgcgcgaat ggtacaccga taccactatc
    26521 ggacaaaatt ctgcgtttta attcagcatt caccgccaaa agcgactaat tttagctgtt
    26581 acagtcagtt gctaaatgca aaggagcatt catgaagcaa acagtttata tcgccagccc
    26641 tgagagccag caaattcacg tctggaacct gaatcatgaa ggcgcactga cgctgacaca
    26701 ggttgtcgat gtgccggggc aggtgcagcc gatggtggtc agcccggaca aacgttatct
    26761 ctatgttggt gttcgccctg agtttcgcgt cctggcgtat cgtatcgccc cggacgatgg
    26821 cgcactgacc tttgccgcag agtctgcgct gccgggtagt ccgacgcata tttccaccga
    26881 tcaccagggg cagtttgtct ttgtaggttc ttacaatgcg ggtaacgtga gcgtaacgcg
    26941 tctggaagat ggcctgccag ttggcgtcgt cgatgtggtc gaggggctgg acggttgcca
    27001 ttccgccaat atctcaccgg acaaccgtac gctgtgggtt ccggcattaa agcaggatcg
    27061 catttgcctg tttacggtca gcgatgatgg tcatctggtg gcgcaggacc ctgcggaagt
    27121 gactaccgtt gaaggggccg gcccgcgtca tatggtattc catccaaacg aacaatatgc
    27181 atattgcgtc aatgagttaa acagctcagt ggatgtctgg gaactgaaag atccgcacgg
    27241 caatatcgaa tgtgtccaga cgctggatat gatgccggaa aacttctccg acacccgttg
    27301 ggcggctgat attcatatta ccccggatgg tcgtcactta tatgcctgtg accgtaccgc
    27361 cagcctgatt actgtcttca gcgtttcgga agatggcagc gtgttgagta aagagggctt
    27421 ccagccaact gaaacccagc cgcgcggctt taatatcgat caccgtggta agtatctgat
    27481 tgccgccggg caaaaatcgc accacatctc ggtatacgaa attgttggtg agcaggggct
    27541 actgcatgaa aaaggccgct acgcggtcgg gcagggacca atgtgggtgg tggttaacgc
    27601 acactaaccg ctgatttacc cggcgcagtc tctcctgcgc cggtgtatta acctatctcc
    27661 tgtaacgcat gtctctggcg ttcgacgata ttggtccaca aattgtcttt atcgtcagtc
    27721 cacaaattaa tcagcaaggc aaaaaagcgt tctgctgccg gagaaagcac ggcatctttg
    27781 cggcgaataa tccccaatgt ccgacgtata acgggttcca ccagtgggat accaataata
    27841 gaagaatagg gcgcatgggg cattgccaga ccaggaagcg ccgaaattcc cagtcccgcc
    27901 tctaccagtc ctaatgacgt cgaaagatga cgcacttcgt aaaaccagtc cagcttccag
    27961 ggcttgtcgg ctagctgttg ttctatcaac aatcggttgc cactggagga acgtacgcca
    28021 atcattttgt aacccactaa ctcctgccat tctacaagct gctttttagc caatggatgg
    28081 tcacgccgac aggccagcac gaacggctcg ttgaccagtg gggtaaaatc aatggatgaa
    28141 tttgtgacgt tgttcatgtt tatgccaaag tctgattcgt tacacagcac cgattccatg
    28201 caattattcg tgccttgttc cagaatccgc actttaatat tgggatacag ctcattaaat
    28261 ttaccgattg ccagcggtaa aaaataaaat actgcggtcg gaatacacgc taacgtcacc
    28321 ataccacgat gataagcatt catatcacga atattaaaaa gcgtttcatc gaatttcttt
    28381 attaattccc gcgcctctgg gagcaacctt tttcctgctt ttgttaacgt aaccttgcga
    28441 gttgtacgct caaatagttg aatatgtaaa tcttcctcca tcttttttat tcttcgtgtt
    28501 aatgcaggtt gcgtaatatt gagtaattta gcggcattgt taaatgaact ggactctgcc
    28561 agtatgacaa atgccttcat acttgataat tcatgcttca ttattactcc gggaaatgga
    28621 agcgacgatt ttgggtggct ggccgttaaa aattttaact gcatttagcc aacttaaatt
    28681 aatgaaaaaa tgttattaat cgttgagcta aagtcattag agatgctttg cccttaatgt
    28741 aaccatatcg caataagtta tgtttttaaa ttgagggcat tattatgaaa aaaataccct
    28801 gcgtgatgat gcgaggtgga acctcgaggg gcgcgttcct gttagcggaa catttacccg
    28861 aagatcaaac gcagcgcgat aaaatattga tggcaattat gggttccggt aacgatctgg
    28921 aaattgacgg tattggcggc ggtaatccac tgaccagtaa agtcgccatt attagccgtt
    28981 ccagcgatcc gcgtgctgat gttgattatc tgtttgctca agttatcgtc catgagaaac
    29041 gtgtcgatac cacgcctaac tgcggcaata tgctgtctgg cgttggggca ttcgccattg
    29101 aaaatggttt gattgcagcg acttcgccag ttacccgcgt acgtatccgc aacgtcaata
    29161 cgggtacgtt catcgaagct gatgtgcaaa cgccaaatgg tgttgtcgag tacgagggta
    29221 gcgccagaat tgacggcgta ccgggtactg ccgcaccggt tgcgctcact ttcctgaatg
    29281 ccgctggaac gaaaaccgga aaagttttcc cgactgataa tcagattgat tattttgacg
    29341 atgtcccggt gacctgtatc gatatggcga tgccagtcgt cattattccg gctgaatatc
    29401 tgggaaaaac aggctatgaa ttaccggcgg aactggatgc cgacaaagca ttattagccc
    29461 gcattgaatc tatccgtcta caagcgggta aagcaatggg cttaggcgat gtcagtaata
    29521 tggttatccc taaacctgtg cttatttctc cagcgcagaa aggcggggca attaatgtgc
    29581 gttattttat gccgcattct tgccatcgcg cgctggcgat aaccggtgct attgctattt
    29641 ccagtagttg tgcattggaa ggtaccgtca cccgacaaat cgtcccttct gtaggatacg
    29701 gcaatatcaa tattgaacac cccagtggtg ggctcgacgt tcatttaagt aatgaaggtc
    29761 aggatgccac gacgttacgc gcatctgtta ttcggacaac cagaaaaata ttttccggtg
    29821 aagtttatct tccctgaaaa gattcgttgt caggataagg acaatcaata aaggacttct
    29881 gtatgagtca tacagaaaga acaggatttt aaatgaataa gaaatcgtta tggaagctaa
    29941 ttctgatatt agcgatccca tgtattattg gctttatgcc agctccggca ggattaagcg
    30001 aactggcgtg ggtgcttttt ggtatttacc tggcggccat tgtggggctg gttatcaagc
    30061 ctttcccgga acctgtcgta ctgttaattg ccgttgctgc ctccatggtg gtggtcggta
    30121 acttatccga cggtgcgttt aagaccaccg ccgtattaag cggttactct tcaggtacta
    30181 cctggctggt gttctcggcg tttaccttaa gcgccgcatt tgtgaccacc ggtttaggta
    30241 aacgtattgc ctatctgctg attggtaaaa tcggtaacac cacgctgggt ctgggttacg
    30301 ttacggtatt cctcgatctg gtattggctc cggcaacacc gtctaacacc gcgcgtgcgg
    30361 gcggcattgt gttaccgatc atcaacagcg tggcggtggc tttggggtcc gaaccggaaa
    30421 aaagtccgcg tcgtgtcgga cattacctga tgatgtccat ttacatggtc accaaaacca
    30481 ccagctatat gttctttacc gcaatggcgg ggaacattct ggcgctgaaa atgatcaacg
    30541 acattctgca cctgcaaatt agctggggtg gatgggcgct ggccgccgga ttgccgggca
    30601 tcattatgct gctggtcacc ccgctggtga tttacaccat gtatccacca gaaattaaga
    30661 aggtggataa caaaaccatc gctaaagcgg gccttgccga actgggaccg atgaaaatcc
    30721 gcgaaaaaat gctgctcggt gtctttgtgc tggcgctgct gggctggatt ttcagtaagt
    30781 ctctgggggt tgatgaatct tcagtggcta ttgttgttat ggcgaccatg ctgctgctgg
    30841 gtatcgttac ctgggaagac gtggttaaaa ataaaggcgg ctggaatacc ttaatctggt
    30901 acggcggtat tatcggctta agctccttat tatcgaaagt taaatttttc gaatggttag
    30961 ctgaagtctt taaaaataac ctggcatttg atggtcacgg taacgttgct ttcttcgtta
    31021 ttattttcct cagcatcatc gtgcgttatt tcttcgcttc cggtagtgcc tatatcgttg
    31081 ctatgttacc ggtatttgcc atgctggcga acgtctccgg cgcaccgtta atgttaaccg
    31141 cgctggcact gttgttctcc aactcctatg gcggcatggt tactcactat ggcggcgcgg
    31201 caggtccggt catctttggc gtgggttata acgatattaa atcctggtgg ttggtcggtg
    31261 cggtactgac gatattaacc ttcctggtgc atatcaccct cggcgtgtgg tggtggaata
    31321 tgctgatcgg ctggaacatg ctgtaaatat actcgtcata cttcaagttg catgtgctgc
    31381 gtctgcgttc gctcacccca gtcacttact tatgtaagct cctggggatt cactcgcttg
    31441 tcgccttcct gcaactcgaa ttatttagag tatatccatt tattttcttg ctgcgcactt
    31501 cacggtgcgc agatatctgg agcatttgat gatcaagtta tctgaaaaag gcgtgtttct
    31561 cgccagtaat aacgaaataa ttgccgaaga acatttcacc ggcgaaatta aaaaagaaga
    31621 agcaaaaaaa ggcactattg cctggtctat tctctcttct cataatacgt ccggaaatat
    31681 ggataaactt aaaattaagt ttgattcatt agcctctcac gatattacct ttgttggtat
    31741 tgtacagacc gctaaagcgt ccggtatgga acgtttcccg ctgccgtatg tgctgaccaa
    31801 ctgccataac tcactctgcg ccgtcggcgg caccattaac ggtgatgacc atgtttttgg
    31861 tttatcggca gctcagcgtt atggcggtat ttttgtgcct ccgcatattg cggtcatcca
    31921 tcaatatatg cgtgagatga tggcaggtgg cggcaaaatg atcctcgggt cagacagtca
    31981 cacccgttac ggtgcattag ggacaatggc agtcggtgag ggcggcggcg agttggtaaa
    32041 acaattgctt aatgacacct gggatatcga ctatccggga gttgttgcgg tgcatctgac
    32101 cggaaaacca gcgccgtatg tggggccgca ggatgtggcg ttggctatca tcggtgccgt
    32161 gttcaaaaac ggctacgtca aaaacaaagt gatggaattc gtaggtcccg gtgttgctgc
    32221 gctctctacc gatttccgta acagcgttga cgttatgacc actgaaacga cctgtttaag
    32281 ttctgtctgg caaaccgatg aagaagtcca taactggctg gcgctgcacg gtcgcggcca
    32341 ggattactgc cagcttaacc ctcaaccgat ggcgtactac gatggctgca tcagcgttga
    32401 tttaagcgcc atcaaaccaa tgattgcgct gccgttccac ccgagcaacg tgtatgaaat
    32461 cgacacactg aaccagaact tgaccgacat tctgcgtgag attgaaattg agtccgaacg
    32521 cgtggcgcac ggtaaagcca aactctcgct gctggacaaa gtggaaaatg gtcgcctgaa
    32581 agtgcagcag gggattatcg cgggctgttc tggcggtaac tacgaaaacg tcatcgcggc
    32641 ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc ttctcgctgg cggtttaccc
    32701 gtcatcacag ccggtgttta tggatctcgc caaaaaaggt gtggtagcag atttgattgg
    32761 cgcaggcgca atcatcagaa ccgcgttctg cggcccatgc tttggcgcgg gcgatacgcc
    32821 aatcaacaac ggtttgagta ttcgccacac cacgcgtaac ttcccgaacc gcgaaggctc
    32881 taagccagct aatgggcaga tgtcagcggt ggcgttgatg gacgctcgtt ctatcgctgc
    32941 gactgcggca aacggtggct atttaacctc tgccagcgaa ctcgattgct gggacaacgt
    33001 gccggagtac gccttcgatg taacgccgta taaaaatcgc gtctatcaag gctttgtgaa
    33061 aggggcggcc cagcaaccgc tgatttacgg accgaacatt aaagactggc cggaattggg
    33121 tgcgctgact gacaatatcg tcctcaaagt gtgctcgaag atcctcgacg aagtgaccac
    33181 caccgacgaa ttgattcctt ccggtgaaac ctcttcttat cgttcaaatc cgattggtct
    33241 ggcggagttt accctgtcac gccgcgatcc aggttatgtt ggcagaagta aagcgactgc
    33301 tgagctggaa aatcagcgtc tggcggggaa tgtcagcgag ctgacagagg tgtttgcgcg
    33361 cattaagcag attgctggtc aggagcatat tgatccgctg caaactgaaa ttggcagcat
    33421 ggtctatgcg gtgaaaccag gcgatggttc tgcgcgtgaa caggcggcga gttgtcagcg
    33481 tgtgattggc ggtctggcga atattgccga ggagtacgcg actaaacgct atcgttctaa
    33541 cgtcatcaac tgggggatgt taccgctgca gatggtggaa gcgccaacct ttgaagtggg
    33601 ggattacatt tacatccctg gcattaaagc ggcgctggat aatccgggta cgacgtttaa
    33661 aggttatgtg atccatgaag atgcgccggt aacggaaatt acgctctata tggaaagtct
    33721 gactgctgaa gagcgcgaga ttatcaaggc gggtagtttg attaacttca ataaaaaccg
    33781 tcagatgtaa aaagcgccat gtgaatgtag gtcgcattcg gcacttattg tcggatgcga
    33841 tgctggcgca tcttatccga cctacaaatc gcatcgaacc gtaggccgga taaggcgttt
    33901 acgccgcatc cggcaaatag ttaatagctc ttacttcttc gcctctgcaa ccactttgct
    33961 acccacgccg cggttattgt attcccacat gcggttgtag ttagtgtcat tcagattgcg
    34021 ctgtatttcg tcgctatcgt caacgctgcc ggtgttaccc gcaaacggac gattagagat
    34081 caccgcatcg ccccacggtt tagccgtgtt aaaaccttcg ttgatggcgc tatcacggat
    34141 caccacctga ccgttggtat tggcatcaac atccagcgag cggcccagtt gtgccacgcc
    34201 atcaccggaa gcattgaaac ggctgtttac ggcgaggaaa ccgtagtaaa tgttggacag
    34261 cgtagccggt gcaaacacat acgcttcttg ctgagtacgt gagttcacca cgcggaattc
    34321 ggtgttatcg aacaccactg cgccgcgacc agaaacgata tccacatccc cttcaatgta
    34381 gctgttggtc accagcgtac gcggctgacg attcgtttcc agacggttct gcacaccgct
    34441 gttggtgaca aagaaggtgt tctgacgacc gagaatgtta acgttgttaa tctgtacctg
    34501 gtcaccgtca gtacgcagtg ccaccgccgg atggttaccc gcatctacgc tatcacccag
    34561 cgtgttttcg atggtcaggt tttgcagttg caggccattg ttttgtgacc agaagaccgc
    34621 agaacacaga acaccgatac tgtcgctgcg tttgctctgg cagctatcgt acatatacca
    34681 cgctggttta cctggcatat atttgccgcg cgggttgacg tcgtgacgcc agtcggcagg
    34741 gctcatgccc ccatcaaggg aaagcccaat cttcacatca atcggttttt cacctgtacc
    34801 gtacagagta attccacctg gagcagcagg gacatacacc gttccctgat actcaccagg
    34861 catcacggca atatactggc gcttgttggt gcgcttgata attgccgcat ctaccgccgc
    34921 ctgaatcgtg gtatgcgtta caccttgagt acccgccggg ccgacaacaa agtcaggttg
    34981 cgcaggcagg gttatcgggg acggattcca cgctgcagca cctggtgtca gggatgcaaa
    35041 atagtgttga gcatcgaaat tctgcgcttc ttttgccgac agaatcgggc gagaagaggt
    35101 accaggcgca gtttgatcag aaggacgttg atcgggtggt gttgagctac aggcggtcag
    35161 cgtcacgcca aaagccaatg ccagcgccag acgggaaact gaaaatgtgt tcacaggttg
    35221 ctccgggcta tgaaatagaa aaatgaatcc gttgaagtct gcttttttat actaagttgg
    35281 cattataaaa aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
    35341 taaaatcatt atttgatttc aattttgtcc cactccctgc ctctgtcatc acgatactgt
    35401 gatgccatgg tgtccgactt atgcccgaga agatgttgag caaacttatc gcttatctgc
    35461 ttctcataga gtcttgcaga caaactgcgc aactcgtgaa aggtaggcgg atccccttcg
    35521 aaggaaagac ctgatgcttt tcgtgcgcgc ataaaatacc ttgatactgt gccggatgaa
    35581 agcggttcac gacgagtaga tgcaattatg gtttctccgc caagaatctc tttgcattta
    35641 tcaagtgttt ccttcattga tatcccgaga gcatcaacat gcaatgttgt agggatggca
    35701 atttttacgc ctgttttgct ttgttcgaca taaagatatc catctacgat atcagaccac
    35761 ttcatttcgc ataaatcacc aactcgctgc ccggtaacaa cagccagttc cattgcaagt
    35821 ctgagccaac atggtgacga ttctgctgct tgataaattt tcaggtattc gtcagccgta
    35881 agtcttgatc tccttacctc tgattttgct gcgcgagtgg cagcgaccgg gtttgttgtt
    35941 atatggcctt cagctattgc ctctcggaat gcatcgctca gtgttgatct gattaacttg
    36001 gctgacgccg ccttgccctc gtctatgtat ccattgagca ttgccgcaat ttcttttgtg
    36061 gtgatgtctt caagtggagc atcaggcagc cccctcctta ttgctttaat tttgctcatg
    36121 taatttatga gtgtcttctg cttgattcct ctgctggcga ggattttttc gtagcgatca
    36181 agccatgaat gtaacgtaac agaattatca ctgttgattc tcgctgtcag aggcttgtgt
    36241 ttgtgtcctg aaaataactc aatattggcc tgtattgctt cagtgattgc tatcctcctg
    36301 tctcggccta aaccaaactc tttacccgtc cttgggtccc tgtagcagta atattgtttc
    36361 ttatataaag gttagggggt aaatcccggc gctcatgact tcgccttctt cccatttctg
    36421 atcctcttca aaaggctacc tgttactggt cgatttaagt caacctttac cgctgattcg
    36481 tggaacagat actctcttcc atccttaacc ggaggaggga atatcctgca ttcgcgcacc
    36541 catcgacgaa ctgtttcaag gcttcttggg cgtcgctggc gtgcgttcca ctcctgaagt
    36601 gtcaagtaca tcgcaaagtc tccgcaatta cacgcaagaa aaagccgcca tcaggcggct
    36661 tggtgttctt tcagttcttc aattcgaata ttggttatgt ctgcatgtgc tatctgcgcc
    36721 catatcatcc agtggtcata gcagtcattg atgttctctg cttcgataac tctgttgaat
    36781 ggttctccat tccattcacc tgtgactcgg aagtgcattt atcatctcca taaaacaaaa
    36841 cccgccgtag cgagttcaga taaaagaaat cctcgtcagt gcgaggatgc tgttcattgc
    36901 tgctatacac ttttttgctc tcaacgtaag cggtagctca ttctgttggg ttggtgcagt
    36961 tgcttttagg aaatgctatt taccccttaa acgtcggctg aaagagctaa aatccatgca
    37021 aaaaatttac gcaattttgt gtattattgt gcagtaagta atgagctatt ttctgcgcaa
    37081 aaaatggatg gtaaatttgt ccggggcagg aaaaatttta tgggcgctaa acatgaaaaa
    37141 agattcgtat ccttatttga tttgcatgac agttttaggc ctgatcttta ttttcctttt
    37201 cttctggtgg cgggcagata tttacagggt cacgtttctt aatcagagta tatcccacta
    37261 ttacattctg tttagcatgg gaatagcttt tctgttatct ctgttttggg ttaagaaggg
    37321 gatagtaaaa caaagcggct ggaagagtct gtcagcatac cttaaggttt atgcagggat
    37381 gtgcatattt gctggatttt ttctgattat accccttaca acactaactt attttttgcc
    37441 tggagagaca tcgtcttatg ttgcaccgta tcggtatact tccggtagtt caaaaagttg
    37501 ttctggagct gaggtggatg accccgatct acatgagaat attcgcattt gctatccgta
    37561 tggcaattat gagtacgata atattatcta tgttgaaaag aaaattaata cattaggtgc
    37621 ggtagtaaca tatgcacaga ccgcgcgtga tgatactgaa tgagatagta tatagcgggc
    37681 aagttttagt taatttatcg aggtaatata atttacctcg actcgtttat tctggtatta
    37741 atatttcgct ttacgatcga tttttatctg atgatatcat gcggttttca tatactgact
    37801 tactgtcttt tctccgttag cgattttctc ctgctcagcg atgattttat ctttggcttc
    37861 tagttaattt cgctcacttc gaacctctct gtttactgat aagttccaga tcctcctggc
    37921 aacttgcaca agtccgacaa ccctgaacgg ccaggcgtct tcgctcatct atgggatcgc
    37981 cacactcaca acaatgagtg gcagatatag cctggtggtt caggcggcgc atttttattg
    38041 ctgtgttgcg ctgtaattct tcaatttctg atgctgaatc aatgatgtct gccatcttcc
    38101 attaatccct gaattgttgg ttaatacgct tgagggtgaa tgcgaataat aaaaaaggag
    38161 cctgtagctc cctgatgatt ttgcttttca tgttcaccgt tccttaaaga cgccgtttaa
    38221 catgccgatt gccagactta agtgagtcgg tgtgaatccc atcagcgtta ccgtttcgcg
    38281 gtgcttcttc agtacgctac ggcaaatgtc atcgacgttt ttatccggaa acggctgtct
    38341 ggcttttttg atttcagaat tagcctgacg ggcaatactg cgaagggcgt tttcttgctg
    38401 aggtgtcatt gaacaagtcc catgtcggca agcataagca cacagaatat gaagcccgct
    38461 gccagaaaaa tgcattcagt ggttgtcata ccaggtctct ctcatctgct tctgctttcg
    38521 ccaccatcat ttccagcttt tgcgaaaggg atgtggctaa cgtatgaaat tcttcgtctg
    38581 tttctactgg tattggcaca aacctgactc caatttgagc aaggctatgt gccatctcaa
    38641 tactcgttct taactcaaca ggagatgctt tgtgcatatc gcctcccgtt tattatttat
    38701 ctcctcagcc agccgctggg ctttcagcgg atttcggata acagaaaggc cgggaaatac
    38761 ccagcctcgc ttcgtaacgg agtagacgaa agtgatcgtg cctacgcgga tattatcgtg
    38821 aggatgcttc atcgccattg ctccccaaat acaaaaccaa tttcagccag tgcctcgtcc
    38881 attttttcga tgaactccgg caccatctcg tcaaaacccg ccatgtactt ttcatcccgc
    38941 tcaaccacga cataatgcag gccttcacgc ttcatacgcg ggtcatagtt ggcaaagtac
    39001 caggcatctt ttcgcgtcac ccacatgctg tactgcacct gggccatgta agccgacttt
    39061 atggcctcga aaccaccgag cctgaacttc atgaaatccc gggaggtaaa cgggcatttc
    39121 agctcaaggc cgttgccgtc actgcataaa ccatcgggag agcaggcggt gcgcatactt
    39181 tcgtcgcgat agatgatcgg ggattcagta acattcacgc cggaagtgaa ttcaaacagg
    39241 gttctggcgt cgttctcgta ctgttttccc caggccagcg ccttagcatt aacttccgga
    39301 gccacaccgg tgcaaacctc agccagcagg gtgtggaagt aggacatttt catgtcaggc
    39361 cacttctttc ctgagcgggg ctttgctatc acgttgtgaa cttctgaagc ggtgatgacg
    39421 ccgagccgta atttgtgcca tgcatcatcc ccctgttcga cagctctcac gtcgatcccg
    39481 gtacgctgca ggataatgtc cggtgtcatg ctgccacctt ctgctcagtg gctttctgtt
    39541 tcaggaatcc aagagctttc actgcttcgg cctgtgtcag ttctgacgat gcgcgaatgt
    39601 cgcggcgaaa tatctgggaa cagagcggca ataagtcgtc atcccatgtt ttatccaggg
    39661 cgattagcag agtgttaatc tcctgcatgg tttcatcgtt aaccggagtg atgtcgcgtt
    39721 ccggctggcg ttctgcagtg tatgcagtat tttcgacaat gcgctcggct tcatccttgt
    39781 catagatacc agcaaatccg aaggccagac gggcacactg aatcatagct ttatgccgta
    39841 acatccgttt gggatgcgac tgccacggcc ccgtgatttc tctgccttcg cgggttttga
    39901 atggttcgcg gcggcattca tccatccatt cggtaacgca gatcggatga ttacggtcct
    39961 tgcggtaaat ccggcatgta caggattcat tgtcctgctc aaagtccatg ccatcaaact
    40021 gctggttttc attgatgatg cgggaccagc catcaacgcc caccaccgga acgatgccgt
    40081 tctgcttatc agggaaggcg taaatttctt tcgtccacgg attaaggccg tactggttgg
    40141 cgacgatcaa caatgcgatg aactgcgcat cgctggcatg aaagcgcgat ggggagacag
    40201 ggcggcatga gacgacagcg acgaagtttc accgacatca tctgcgaaaa ctgcaaatac
    40261 cttccaacga aacgctccag aaataaacgc aagccaatcc caaaagaatc tgacgtaaaa
    40321 accttcaact acacggctca cctgtgggat atccggtggc taagacattg tgcgaggaaa
    40381 acaaggtgat tgaccaaaat cgaagttacg aacaagaaag cgtcgagcgg gctttaacgt
    40441 gcgctaactg cggtcagaag ctgcatgtgc tggaagttca cgtgtgtgag cactgctgcg
    40501 cagagctgat gagcgattcg aatagctcga tgcacgagga aaaagatgat ggctaaacca
    40561 gcgcgaagac gatgtaaaaa cgatgaatgt cgggaatggt ttcaccctgc attcgctaat
    40621 cagtggtggt gctctccaga gtgtggaacc aagatagcac tcgaacgacg aag
//
LOCUS       DAZFBR010000029        40416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFBR010000029 DAZFBR010000000
VERSION     DAZFBR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.029"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..330
                     /locus_tag="ACHJZ2_001769"
     CDS             <1..330
                     /locus_tag="ACHJZ2_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4567894.1"
                     /translation="IISEFMAEPPQGKIRIAGGDGLPAWADIPAPTHEELIEITESER
                     QLLINQANDYMNSKQWPGKAAIGRLKGEELAQYNLWLDYLDALELVDTSGAPDIKWPT
                     PPAVQAR"
     gene            complement(302..904)
                     /gene="tfaE"
                     /locus_tag="ACHJZ2_001770"
     CDS             complement(302..904)
                     /gene="tfaE"
                     /locus_tag="ACHJZ2_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4567895.1"
                     /translation="MDNAVLNSEFIATKAGNITVYNYDSETREYISTSTEYLAVGVGI
                     PACSCLDAPGIHKVGYAICRSVNLNSWEYVPDHRGEIVYSTETGESKEITAPGDYPEN
                     TTTIAPLSPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYTDAVTATDTSTAPDVIWPELPEA"
     gene            complement(904..2403)
                     /locus_tag="ACHJZ2_001771"
     CDS             complement(904..2403)
                     /locus_tag="ACHJZ2_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4567896.1"
                     /translation="MTVKYYAILTNQGAARLANATMLGSKLNLTQMAVGDANGVLPTP
                     DPAQTKLINQKRIAPLNLLSVDPNNQSQIIAEQIIPENEGGFWIREIGLYDDEGVLIA
                     VANCPETYKPQLQEGSGRTQTIRMILVVTNTEAITLKIDPSVVLATRKYVDDEVLELR
                     LYVDDQMRNHIAAQDPHTQYAQKHNPTFTGEPKAPTPDAGNNTTRIATTAFVQAAITA
                     LINGAPATLDTLKEIAAAINNDPKFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAAMVGSAPAALDTLNELAAALGNDPNFSTTVLNALAGKQPLDNT
                     LTNLSGKDVAGLLAYLGLGEGSALPVGVPVPWPSATPPTGWLKCNGAAFDKVKYPRLA
                     TAYPSGKLPDLRGEFIRGWDDGRGIDTGRALLSIQSDEVRKLALKYWGPASNSSPSKT
                     FALSDSAGGGLYTDGISQASGGIINAFQLPGGNETRPRNVAFNYIVRAA"
     gene            complement(2400..3011)
                     /locus_tag="ACHJZ2_001772"
     CDS             complement(2400..3011)
                     /locus_tag="ACHJZ2_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO4567897.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWSFSVDRWDDNWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYQELERLVADAKPASRHLTGLAISLSTTGNIF
                     AGAGCYHGDALTVYPYTPEAIIVGGDYFPASAIHLIDNLRVNA"
     gene            complement(3004..3912)
                     /locus_tag="ACHJZ2_001773"
     CDS             complement(3004..3912)
                     /locus_tag="ACHJZ2_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFO4567898.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAAVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNNTQASFCTEYSVVTGGSDE"
     gene            complement(3917..4264)
                     /locus_tag="ACHJZ2_001774"
     CDS             complement(3917..4264)
                     /locus_tag="ACHJZ2_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO4567899.1"
                     /translation="MTARYLGMNRSDGQTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(4261..4896)
                     /locus_tag="ACHJZ2_001775"
     CDS             complement(4261..4896)
                     /locus_tag="ACHJZ2_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4567900.1"
                     /translation="MNTLANIQELARALRNMIRTGVIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTASGIKTASVTASDSVTATVPVVMVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(4963..5415)
                     /locus_tag="ACHJZ2_001776"
     CDS             complement(4963..5415)
                     /locus_tag="ACHJZ2_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO4567901.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQAAMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(5408..5875)
                     /locus_tag="ACHJZ2_001777"
     CDS             complement(5408..5875)
                     /locus_tag="ACHJZ2_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4567902.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLAASLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPTPPEPVTRPMELYINGELVSKWDE"
     gene            complement(5838..6083)
                     /gene="lysC"
                     /locus_tag="ACHJZ2_001778"
     CDS             complement(5838..6083)
                     /gene="lysC"
                     /locus_tag="ACHJZ2_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFO4567903.1"
                     /translation="MLCAGCTSAPPAQTPVIVANACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(5983..6408)
                     /gene="lysB"
                     /locus_tag="ACHJZ2_001779"
     CDS             complement(5983..6408)
                     /gene="lysB"
                     /locus_tag="ACHJZ2_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFO4567904.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASEQQ
                     ATITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACADASDCRQRLPESEPLPDAGQ"
     gene            complement(6396..6821)
                     /locus_tag="ACHJZ2_001780"
     CDS             complement(6396..6821)
                     /locus_tag="ACHJZ2_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFO4567905.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTALFLQPVPWRRRPVALVVMVATFIS
                     LVAAGWIFTALIYLVASVFFRLIRTACRQRFEGREPCQS"
     gene            complement(6836..7333)
                     /locus_tag="ACHJZ2_001781"
     CDS             complement(6836..7333)
                     /locus_tag="ACHJZ2_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO4567906.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     QEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(7333..7614)
                     /locus_tag="ACHJZ2_001782"
     CDS             complement(7333..7614)
                     /locus_tag="ACHJZ2_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4567907.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKSRGKP"
     gene            complement(7618..7821)
                     /locus_tag="ACHJZ2_001783"
     CDS             complement(7618..7821)
                     /locus_tag="ACHJZ2_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO4567908.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(7821..8330)
                     /locus_tag="ACHJZ2_001784"
     CDS             complement(7821..8330)
                     /locus_tag="ACHJZ2_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO4567909.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(8430..9173)
                     /locus_tag="ACHJZ2_001785"
     CDS             complement(8430..9173)
                     /locus_tag="ACHJZ2_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFO4567910.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            complement(9177..10250)
                     /locus_tag="ACHJZ2_001786"
     CDS             complement(9177..10250)
                     /locus_tag="ACHJZ2_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO4567911.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGRTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            complement(10305..11159)
                     /locus_tag="ACHJZ2_001787"
     CDS             complement(10305..11159)
                     /locus_tag="ACHJZ2_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO4567912.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVVELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMENAFSALKQDVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            11333..13105
                     /locus_tag="ACHJZ2_001788"
     CDS             11333..13105
                     /locus_tag="ACHJZ2_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFO4567913.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            13105..14139
                     /locus_tag="ACHJZ2_001789"
     CDS             13105..14139
                     /locus_tag="ACHJZ2_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4567914.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFATGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDALQDR
                     DYIDSIRENMANSKGRNNFKNLFLYAPQGKPDGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            14352..14621
                     /locus_tag="ACHJZ2_001790"
     CDS             14352..14621
                     /locus_tag="ACHJZ2_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567915.1"
                     /translation="MQMHEHAEGAPVPAALTRDWEGKRMRFDAVMHAELKMAEHTEKT
                     HKKTGIQVAGLSRLHCGYWPRNAPIILVRQQVATISKKTVVHGFS"
     gene            complement(14652..14846)
                     /locus_tag="ACHJZ2_001791"
     CDS             complement(14652..14846)
                     /locus_tag="ACHJZ2_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567916.1"
                     /translation="MHNSLSSIKDPYADWAKRLTVMASNNDLSSREVESYTEKMVEQA
                     SKDELTVVIKHLLNHIRMNK"
     gene            14845..15282
                     /locus_tag="ACHJZ2_001792"
     CDS             14845..15282
                     /locus_tag="ACHJZ2_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567917.1"
                     /translation="MSKNLELFNQQTAEIFAVLWDNFPVPQVITYKKFNAALPDDYFD
                     QLNSPEMKALNQLRSVVDGTFTFLSENGYILYGTDHQTGFHDVRLTEKALAVLNKKPE
                     ALGGNETMGDKIISAVKDGTPGVIAGAVTNLLTLGVNFVTSAS"
     gene            complement(15407..16357)
                     /locus_tag="ACHJZ2_001793"
     CDS             complement(15407..16357)
                     /locus_tag="ACHJZ2_001793"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HFO4567918.1"
                     /translation="MKKRKKLKVNTKNKSYDIMDSPFYKLKSKRKLANLLCVSLDDLY
                     SLRKDEGNYSVFEQLSKKGKARKIQKPLEKLELVHTRIASLLSRIALPEYLHSGKKKC
                     SNVTNAKAHLNNEKMMTTDIKAFFPSTTRGMIFSFFFSVMKMSSDVADVLSHICTCHD
                     RLPTGSRISMPLAYFANSRMFGEIYQLCQKLRVNMTVYVDDLTFSGSNVNRLFCAVIR
                     KIVNKHGHVIHPTKTKLYARDKPKLVTGVIVLGNALKVRNEQHFLMAREIDYWKIIKD
                     AENARETIIAKKLFGRLHSMGVIEQRYKSKVLTLKANTAN"
     gene            complement(16335..16643)
                     /locus_tag="ACHJZ2_001794"
     CDS             complement(16335..16643)
                     /locus_tag="ACHJZ2_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4567919.1"
                     /translation="MLHKALRLIRQYHKESIVELSSSLGIPKDKIVSLESGVWSPSIE
                     VLERYASHFDIPVSSLVFFSESLGTQGRLSKRLRLNLAGKVLDVLEWVSNKNEKTEKA
                     "
     gene            complement(16680..16973)
                     /locus_tag="ACHJZ2_001795"
     CDS             complement(16680..16973)
                     /locus_tag="ACHJZ2_001795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567920.1"
                     /translation="MGIRTLWATKGSWSRGLVLFNLILMRANHSVGADIPFAHYGHKE
                     QIMKNDKLTNEQQAQNKSNLKKWAGYAWKLIKWGIRLFDLVTRALDYFEGGDE"
     gene            complement(17244..19532)
                     /locus_tag="ACHJZ2_001796"
     CDS             complement(17244..19532)
                     /locus_tag="ACHJZ2_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO4567921.1"
                     /translation="MAVKASGHFVPSSAFAAGTGKTFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEEICDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTTPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHDLRTPNRQ
                     QRSGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWEIEALARGATVNYDG
                     KKFTYMVINEWFGFRDEESFFM"
     gene            complement(19522..19797)
                     /locus_tag="ACHJZ2_001797"
     CDS             complement(19522..19797)
                     /locus_tag="ACHJZ2_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO4567922.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGTHGR"
     gene            complement(19794..20018)
                     /locus_tag="ACHJZ2_001798"
     CDS             complement(19794..20018)
                     /locus_tag="ACHJZ2_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFO4567923.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCCASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(20018..20320)
                     /locus_tag="ACHJZ2_001799"
     CDS             complement(20018..20320)
                     /locus_tag="ACHJZ2_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO4567924.1"
                     /translation="MSCSRSIVLLNNALKIAVMENGDLSLIQLGLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADEFKKLNDKRNW"
     gene            complement(20320..20544)
                     /locus_tag="ACHJZ2_001800"
     CDS             complement(20320..20544)
                     /locus_tag="ACHJZ2_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFO4567925.1"
                     /translation="MHTVSENQCGIYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(20608..21108)
                     /locus_tag="ACHJZ2_001801"
     CDS             complement(20608..21108)
                     /locus_tag="ACHJZ2_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFO4567926.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQETCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(21105..21275)
                     /locus_tag="ACHJZ2_001802"
     CDS             complement(21105..21275)
                     /locus_tag="ACHJZ2_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4567927.1"
                     /translation="MNEPRCIAQLLRNESSRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(21286..21561)
                     /locus_tag="ACHJZ2_001803"
     CDS             complement(21286..21561)
                     /locus_tag="ACHJZ2_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HFO4567928.1"
                     /translation="MSKQVTLMTDAIPYQEFAKLIGKSTGAVRRMIDKGKLPVIDMTD
                     PQSASGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            21676..21975
                     /locus_tag="ACHJZ2_001804"
     CDS             21676..21975
                     /locus_tag="ACHJZ2_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4567929.1"
                     /translation="MSNTISEKIVLMRKSEYLSRQQLADLTGVPYGTLSYYESGRSTP
                     PTDVMMNILQTPQFTKYTLWFMTNQIAPESGQIAPALAHFGQNETTSPHSGQKTG"
     gene            22091..23104
                     /locus_tag="ACHJZ2_001805"
     CDS             22091..23104
                     /locus_tag="ACHJZ2_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4567930.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPTGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(23369..23686)
                     /gene="yegR"
                     /locus_tag="ACHJZ2_001806"
     CDS             complement(23369..23686)
                     /gene="yegR"
                     /locus_tag="ACHJZ2_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO4567931.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            24092..24991
                     /gene="yegS"
                     /locus_tag="ACHJZ2_001807"
     CDS             24092..24991
                     /gene="yegS"
                     /locus_tag="ACHJZ2_001807"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO4567932.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(25073..25846)
                     /locus_tag="ACHJZ2_001808"
     CDS             complement(25073..25846)
                     /locus_tag="ACHJZ2_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4567933.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(25952..26992)
                     /gene="gatD"
                     /locus_tag="ACHJZ2_001809"
     CDS             complement(25952..26992)
                     /gene="gatD"
                     /locus_tag="ACHJZ2_001809"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO4567934.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(27040..28395)
                     /locus_tag="ACHJZ2_001810"
     CDS             complement(27040..28395)
                     /locus_tag="ACHJZ2_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO4567935.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQVALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(28399..28683)
                     /gene="gatB"
                     /locus_tag="ACHJZ2_001811"
     CDS             complement(28399..28683)
                     /gene="gatB"
                     /locus_tag="ACHJZ2_001811"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO4567936.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(28714..29166)
                     /gene="gatA"
                     /locus_tag="ACHJZ2_001812"
     CDS             complement(28714..29166)
                     /gene="gatA"
                     /locus_tag="ACHJZ2_001812"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO4567937.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(29176..30438)
                     /gene="gatZ"
                     /locus_tag="ACHJZ2_001813"
     CDS             complement(29176..30438)
                     /gene="gatZ"
                     /locus_tag="ACHJZ2_001813"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO4567938.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(30467..31321)
                     /gene="gatY"
                     /locus_tag="ACHJZ2_001814"
     CDS             complement(30467..31321)
                     /gene="gatY"
                     /locus_tag="ACHJZ2_001814"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO4567939.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(31620..32672)
                     /gene="fbaB"
                     /locus_tag="ACHJZ2_001815"
     CDS             complement(31620..32672)
                     /gene="fbaB"
                     /locus_tag="ACHJZ2_001815"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO4567940.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            32929..34206
                     /gene="yegT"
                     /locus_tag="ACHJZ2_001816"
     CDS             32929..34206
                     /gene="yegT"
                     /locus_tag="ACHJZ2_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO4567941.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            34203..35207
                     /gene="yegU"
                     /locus_tag="ACHJZ2_001817"
     CDS             34203..35207
                     /gene="yegU"
                     /locus_tag="ACHJZ2_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO4567942.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            35204..36169
                     /gene="yegV"
                     /locus_tag="ACHJZ2_001818"
     CDS             35204..36169
                     /gene="yegV"
                     /locus_tag="ACHJZ2_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO4567943.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(36143..36889)
                     /gene="yegW"
                     /locus_tag="ACHJZ2_001819"
     CDS             complement(36143..36889)
                     /gene="yegW"
                     /locus_tag="ACHJZ2_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4567944.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(36941..37759)
                     /gene="yegX"
                     /locus_tag="ACHJZ2_001820"
     CDS             complement(36941..37759)
                     /gene="yegX"
                     /locus_tag="ACHJZ2_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO4567945.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIHIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(37824..38624)
                     /gene="thiD"
                     /locus_tag="ACHJZ2_001821"
     CDS             complement(37824..38624)
                     /gene="thiD"
                     /locus_tag="ACHJZ2_001821"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO4567946.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(38621..39409)
                     /gene="thiM"
                     /locus_tag="ACHJZ2_001822"
     CDS             complement(38621..39409)
                     /gene="thiM"
                     /locus_tag="ACHJZ2_001822"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO4567947.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(39444..39540)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            39743..39982
                     /locus_tag="ACHJZ2_001823"
     CDS             39743..39982
                     /locus_tag="ACHJZ2_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HFO4567948.1"
                     /translation="MTETQIYENIKQAISSAPRNSQTMEMHLQMIKYADHLKNVTAKE
                     FCEGVGLKASFATEFSKMRNLTERLKAAGLDTTKL"
BASE COUNT         9847 a        10724 c         9842 g        10003 t
ORIGIN      
        1 attatcagtg aatttatggc tgaaccgcca caaggaaaaa tccgtattgc cggaggtgat
       61 gggctgcccg cgtgggcgga tattcctgcc cctacgcatg aagaacttat tgaaattact
      121 gaatcagaaa gacagctact aattaatcag gccaacgatt atatgaacag taaacaatgg
      181 cctggtaaag cggctattgg tcgtctgaaa ggcgaggaac tggcgcaata taatctgtgg
      241 ctggattatc tggacgcact ggaactggtc gatacttccg gtgcgccaga tattaaatgg
      301 cctacgcctc cggcagttca ggccagatga catccggcgc ggtgctggta tctgttgccg
      361 tcaccgcgtc agtgtaatcc agcacggcgt taagtcgggt tgtttctgcc tgcgtcagtt
      421 tccgtccggc ctgcaatttc agttgaatca gactgatgga agccatagca gcatcaatca
      481 gtgactggcg ctgtgcttct gccgcttcga ctgcggcgct atgctgtgcc tcagtatcgg
      541 tcacccattt ctcaccatcc catttatcgt atggagataa aggggcgata gtggttgtat
      601 tttcagggta atcacccgga gctgtgattt cttttgattc tcctgtttct gtgctataga
      661 cgatttcacc gcgatggtct ggcacatatt cccatgaatt taaattcaca gaacgacaga
      721 ttgcataacc aaccttatgt atgccaggtg catcaagaca ggaacatgct ggaataccga
      781 cccccacagc aagatattca gttgatgtgg aaatatattc ccgtgtttca ctatcatagt
      841 tatagacggt aatattcccc gccttcgtgg caataaactc gctatttaat acagcattat
      901 ccattatgca gccctcacga tatagttaaa tgcaacattt cgtgggcggg tttcgttacc
      961 gccagggagc tgaaaagcgt tgataatccc accgcttgcc tgacttattc catccgtgta
     1021 caacccgcca ccggcactgt cgctgagtgc aaatgttttt gatggtgaac tgtttgaagc
     1081 tggtccccag tattttaatg cgagctttct gacttcatca ctctgaatac tcaataaagc
     1141 acgccctgta tcaatgccgc gcccatcatc ccagccacga ataaactcac cgcggagatc
     1201 aggtagtttc cctgatggat atgctgtagc aagacgggga tatttaacct tatcaaaggc
     1261 agcgccattg catttcagcc agcctgttgg cggcgtagct gaaggccacg gaacagggac
     1321 accaacaggt aatgcagagc cttctcccaa accaaggtat gcgagaagac cagccacatc
     1381 ctttccactc aaattagtaa gcgtattgtc cagcggttgt ttgcctgcca gtgcattaag
     1441 cactgttgtt gaaaagttag ggtcattccc cagcgccgcc gccagttcat tcagtgtatc
     1501 cagtgccgca ggggcagaac ccaccattgc cgcaattgct gatttcacaa aagccgtggt
     1561 ggcaatctgt gtattgttga ccgactgtgc cgcagtaggt gctgttggcg ttccggtaag
     1621 tgcgggactc gacagcggcg ctttcagtgc cagcgcattg ttaatggtgg tgctgaattt
     1681 cgggtcattg ttaatggctg cggcaatttc tttcagtgtg tccagcgtgg ctggcgcgcc
     1741 gttaatcaga gcggtaatag cagcctgtac aaacgcagtg gtcgcaatcc gcgtggtgtt
     1801 atttcctgca tcaggcgtcg gcgcttttgg ttctccggta aatgtcggat tatgtttctg
     1861 tgcatactgg gtatgaggat cctgtgcggc aatgtggttt ctcatctggt catccacata
     1921 cagccttaat tccaggactt catcatccac gtatttacgg gtcgccagta ccaccgacgg
     1981 gtcgattttc agcgtgatgg cttcggtatt cgtgacaacc agaatcatgc ggatagtctg
     2041 ggtacgacca ctgccttcct gtaactgcgg tttgtacgtt tccgggcagt tcgccaccgc
     2101 aatgagtacg ccttcatcat cataaagccc aatctcacgg atccagaatc cgccctcgtt
     2161 ctcagggatg atttgctccg caataatctg gctctggtta ttcgggtcaa cactcagaag
     2221 attcagcggc gcgatgcgtt tctggttaat cagttttgtc tgtgccgggt ctggtgtcgg
     2281 caagacacca ttcgcatcac caacggccat ttgcgtcaga ttcagcttac tgccgagcat
     2341 cgtcgcgtta gccagccgtg ctgcgccctg attagtcaga atggcgtagt atttcactgt
     2401 catgcgttta ctctcaggtt atcaattaaa tgaatggccg aggccgggaa ataatcccct
     2461 ccgacaataa tggcctccgg ggtgtaggga taaaccgtca gggcgtcacc gtgatagcat
     2521 cccgcaccgg caaaaatgtt gccggttgta cttaaactga tagccagtcc cgtcagatgg
     2581 cggcttgccg gttttgcatc agcaacgaga cgctccagct cctgatacat ttcttcggta
     2641 ataccctgct caagcacgcc aacaacgata cggaacgtcc ccggctcctc gttgagctgc
     2701 caccactccc tcacctcaat cagatagccg agcggctcca ccacacggcg aatcgcacca
     2761 atagtgccct tatgacagtg gatgaaatac gcatcgcgga taacagcgcg ttttgtcgct
     2821 tccggccaat tatcatccca gcggtcaacc gaaaatgacc acgccagcca cggcagcaga
     2881 tttgccgggc aggtgtccgg gttccacagc tcacgaatac tgaccggcgt tttttcaatt
     2941 tccgcacagg cttttgcggc ggcaacttca agcggtgatg agccggtcgg cagcagtcgc
     3001 gaatcactca tccgagcctc cggtcacaac gctgtattcg gtacagaaag acgcctgcgt
     3061 attgttgagc acgatgtcgg ccagtggtgc agccagttcg acacgctgca cgccttccac
     3121 atgcaaagcg gcataaatgg cagacagacg gatgtcgcgc cccagccggt gctgtgccgt
     3181 gatgtacgct tccagttttt tcacggcagc agcgcggatg ggttcgcttt cgggaccagg
     3241 gtaaaggtaa agcgtggcgt ttatctggta ttcaacgatg gcggcagact gcacggtcac
     3301 gcggtcggcc accggcctga cgtcctcgcc attaagggcg ttacgcaccg ccgccagcag
     3361 gtcttcggat gccacaccgt tattttcgcg agacagcacg gagatggtga cgcaggccgg
     3421 agaagggctg gtgacagaga tatctgcgac acgcccgtcg gcactgcgac catgatactg
     3481 ataggcaccc accgacccgg cgacgcttaa accttcaaac gcctgctgaa tacgcagacg
     3541 ataatcggtg tcagattcca tcactgccgg tgtcggcggg atggtcgaat catctgccgg
     3601 ggtgatagtc aggcgcgtgg tgttgtaatt ggcaccaatc acatcaaggt cattaccggc
     3661 ggcacaggcc agcatcaccg cccgtgcggc ctcattcaca cgctgacgcc agataagctc
     3721 acgataagca ttttcctcca gcagtttgac gagaggctca gattccagcg tcagggtacg
     3781 ggcgaccgcc tcctgctggt cttccgggta aagggaaatc agtgtcgcct tgcgttcggc
     3841 aagaatggtt tcaaagtcca gctcctcgac cacatccggt gcgggtagct ggttcaggtc
     3901 gataatcggc atggtttcaa ctcacaggga tggttaacga aagtggctgg ccggtgtcgt
     3961 tgtgctggcc ggttaatgta actgtcattc gcccgtcaaa gctgcgcgcc gtggtgacgg
     4021 atgacagggt gacgcggggt tcccatttca gcaccgccat gtaacaggcg accttaatct
     4081 gcaactcaag tgccggggtc tgcggctggt caatcattga cgccagcaac gagccgtaat
     4141 cacgacgcat cacccgtgag ccgaccggtg tgcgcaggat atcgccgata ctctggctga
     4201 tatgctcaag gtcagtgaca gtctggccat cactgcgatt cattccgaga taacgcgctg
     4261 tcatagagga ctcccggttg tgccgccgct gtcgccgggg tgtttatggg tatgcagtac
     4321 cttaccgttt gatgagagtt caccgccggt gtgttcaatg ttgccgcgca tcgtcccgcc
     4381 cttctgcact tccagcgtgc cggtaatcag cttgtttgtg cagaccacct ccggtgtgtc
     4441 cagggtgacg cgggttgatg ctttcaccat gaccaccggc accgtggcag taacagaatc
     4501 agaagccgtc acgctggccg ttttaattcc gcttgccgtg agtgcactgg tttcaggttc
     4561 atactcaatc accgccccgt caggaaaacg gatatgcagg gcatccgccg acgcagacgg
     4621 cgcggggtta tcaccggaat aaatccccgg cagaacgaac gccgtgtcga gttcaccgcc
     4681 cacggccaga atcagcacct gttcccccac ggaaggtgcc caccatgtgc gcgaacgacc
     4741 ggcgcgatgg gtcagccact gcagccagtc ggtacacatg ccgccggtct gcacacggca
     4801 gcgaccggcg ttaaggtcgg tttcgacgat aacgccggtg cgaatcatgt tgcgcagtgc
     4861 gcgcgcgagt tcctgaatat ttgcgagagt gttcataacg ggaaggatgc cgccgggtca
     4921 taccggcggc aatgtgacga tgaggtgtcg ggaatggcac aactaacggt cgaggtgcgc
     4981 cagaataatc tcttcaatca tctgcacatc ctcaccggtg aagccgagca gagggcgcgc
     5041 cggataatca attttcttac cgtctttccg gttttcttcc gacagaccaa actgatgcac
     5101 actggcgatt ttcggtgact tcccgccgta aaattccatt gctgcctgtt ccgggctggc
     5161 gcggatatgc aaaaaacgac tggtgataag tttcgcaaac atttttcgct taacgcgacc
     5221 ggtctttttt ctggcgctct gctgctggcg tggcgcgtag ggtgtgccgt ccggggcttt
     5281 ctgtgccatc acccgacgct gctgactctg ccgcagacgt ttcgccagtt cggcactcag
     5341 tcgccgacgc cctgacggtg acagcgattc aatcagtccg gtcagccggt cttcaaaacg
     5401 cttaaactca ttcatcccac ttgctcacca gttcgccatt gatataaagc tccatcgggc
     5461 gggtgaccgg ctccggcggc gtgggttccg ggatattctt cacatgcagc gcgccgtcca
     5521 cctcactgac cagcgtgcgc tcggtcagca tcaggctgat gctgatatca aagctgctgt
     5581 cattgttgat gtctgcataa aacgtgaaac cttttttctg gccttcgtcg gtggtcatga
     5641 tgtcgggctg attttcccgc agccacgcca gcaccggcac gatgagcagg tcaaaatcac
     5701 cggtaaagtc ggtcacaatg acattgagcg tgtaacgctt ttcgaatgac aacgacgccg
     5761 ccagcgtgga ggcaatactc ccgttatcca cgaatatccg aagcatctct ggactggttt
     5821 tcagcaccgt gacggcatca gtcagcgccc tgcgcaggct gtcgggtttg agcatcgttt
     5881 tcgtcctgac agtgtttaat catttttacc tggctggcac agcgtgccag cgcgttctca
     5941 agctgccgga tatcggcact taaatcgccg ttcgtctgcg ggtcactgcc cggcatcggg
     6001 caaaggctca ctttcgggca ggcgttggcg acaatcactg gcgtctgcgc aggcggggcg
     6061 ctggtgcaac cggcgcacag catcaggcag gtcagcgccg taccagcggc gaaaatcttc
     6121 gttttcatta agtaacctcg tgatggtttt ctcgcgctgt gcttcacgct tcgcggcgtt
     6181 ctccagttcc tgacgcagtg ccacctgcgc cagctcgttt ttgtctgccc tggtgagggc
     6241 aacatgaagc tgatttttca gcatggtgat ggtcgcctgc tgttcactgg cgacgttgtt
     6301 cgccctgtcc agcgaggcgc gcaggctggc atttttgtgt ttcaccagaa acagaccggc
     6361 caccgccagc gataacaaca cgaccagcac aatcatcagc tttgacatgg ttcccgcccc
     6421 tcaaaacgct gacggcaggc cgtacgtatc agccggaaga acaccgatgc cacgagataa
     6481 atcagcgcgg taaaaatcca cccggcagca accagcgaga taaacgtcgc caccatcact
     6541 accagagcca ctggccgcct gcgccacggc accggctgca aaaacagcgc cgtgacaatt
     6601 ttcacggcca gcgattccgg cggcagctcc cgcccgtagc gttccagcac atactcagtg
     6661 gcatacaccc cgacaccacc ggcaaccaca cagataaccg tcgccagaat cgcccaggca
     6721 gcgacaaaac tgacggccac gctctgcgga taaatcaggg acagtgccag catcagcgcc
     6781 agcgacacgt tcagcatcag tgaaagggat aatttcttca tggtgtttac tccgtttaag
     6841 ccggtacgcc gccagcggta cgccagacgg tgaccagttt ttccagtgaa tgctcacgct
     6901 gaccgtaacc ggcacccggc agggacgccc agatattgcg acagcgtgaa atggcgcgct
     6961 caatgcgtcc cgcccggatg tcatccagtg caccgcgttc gcggatcaac tgaatggcga
     7021 gtctgtcctg tgacaacgga ctgaaatccg gcagggcaag ctgtttgcgg taatgcggcc
     7081 agaacaggta aagctgctga tagcgaccgg aggccgtgga tttttcaccg cgacggttaa
     7141 acaccttcgc cggtcgacca tgtgcgaacg ggtggtcact gtagtcggtg aaaatttcct
     7201 gctttccgtc cagtccggtg actatcacgt catagccccg gtttttcgtc agcggatggt
     7261 tcgccgtccc ttcggacacg gccagcatgt cgagaaaggc ggcgatattc tgatgcgtgt
     7321 taattaccgg cattacggtt tccccctgct cttaaagcgg cgctgaatgg caatctcaat
     7381 cacctgataa ccggcgatac ccagcatgga gccgatgccg cacaccgcag gcagtgacag
     7441 gtcaggaaac tgcaccagaa caacaccggc aaccatcgag acaaaaccac cgagcaacat
     7501 gcgcccgata aacagacgcg gggtgatggg ttcaccaccg gcaagcacct tgccgacaac
     7561 aatcagcacc ccaatcatga aaagcgacag gacgcttttt tcttctgctg tcatgcgtta
     7621 ctcccacaga ttgacagttt cagccacggg cgcggtctga acgtcgggca gttcgacggc
     7681 ggtgccatgt ggcagcaccg cccccagttc agccagtccc ggatttgcgg cgagcacggc
     7741 ttcgaccacg ccctcagtgc gcccgtaata ccggacacaa atggcgtcga gcgtgtcgcc
     7801 ctgtagcgca aaggtcttca tcagatttga ctcacgatgc agcgcggctt gtcctggata
     7861 cgcgccaccg cccagcgcat atcccgccac agctcatcaa tggtgctgtc aatgctgtcg
     7921 gccttcttgt cgcctttcgc actggcatcc acgccgcgat aacgctcata aagcgatgcg
     7981 gtcgccatcg cacacacggc gcgctcgtag taaaaaactt tgatgctttc accgtcgatg
     8041 tcgtccgccg ggacgtccgc cagacgcgta aaaccggcgg caattttctg ttcgcggtac
     8101 tcgtacagct ccgcattcgt ttcagccatg cctgacttga tggcctcacg cagacgggcg
     8161 ggggcgacgg tctgctcaag gcgcatacgt tcccggacgc gcttcgggtc gatatcggga
     8221 aaaaagaacg tgtttttaat caccggctcg tcgcctgccg gttgcgggat gaccaccgta
     8281 ccctcaccgg acacgggagc ctcctttcgc ggaataatca gcgtcatcat gactacctct
     8341 gaaaagtcgg gcggtggacg ccggtgcagt gtcaggtgat tcaccctcac tgaccggcgt
     8401 gccgccctgg cgcggggcgc attcggttgt taactggctt tctttttcgg gcgtccacgt
     8461 tttgccggtg tcacgctccg ggtcttacgc ggggcacggg tggccgcttt tggctgcggc
     8521 tccggcttcg gtttcagctc ccgctccagt cgttcaatct cttttttgac gcctgcctga
     8581 cagtcgagct gtgtcgcacg ttgcaggtga gccagcgcac cggcggcatc accagcgtca
     8641 cgcagaaaca gaccggtgat tttgtgcagc tttgcgcgca cttcatcagg catgtcagcc
     8701 gtggcggtca gttcaagggt gtccgtcagc aggcgggtat ccacagactc accggcagcg
     8761 tgggcacgca tggccgcaag cgcgacctcc tcggtgaaca tgtacggcgg ggtgcggcgg
     8821 tgttttcccg gcatggtcag accgtacttc agggcataac gggcaatctc cagcgcaccg
     8881 gcaatatcgc cggtatccag acgccacagc atgaccgtca tcagaatgtc atcctgtgca
     8941 cctttgccct gctccagaac gccgttcacc cacggcaacc agaacggcag cagttcgcgt
     9001 tttttcgcgg ccttcagctc ttttgaataa atcgctttca gtgtgcgctg gtctgcggcg
     9061 agcttaacca gcatctgctc atagacagtt gcatgtcgca gcggggcggc ttcccgctgc
     9121 gcggtcatcg ctgccgagac ccgcatcatg tggcgctgtg cgggactcgt catcggttac
     9181 gctcccggct ctgcggtcgc cttagccggt gtggagaaat caccgacctt aattttttcc
     9241 accagacaac cggcggcgta gtcttccacc acgtaatcaa tgttcattga ctcgtagttc
     9301 tccacgcggt cgagtttcgg gttttcctca atcacgcggc gatggctgtc atccatgtag
     9361 tagatggaca ggttttccag cttcgtgatg agcatcgcat ccgccgggaa gtacgggacg
     9421 cgtaccgctg gcaggttacc gatgcgtttc tggctgatga tgacgtcagc ggccagcatt
     9481 tcgctgttgt cctgctcctt gttaacgatg gggaaatact tgtccgccag tagctgacgc
     9541 cccacaatca ccacaaggtc agggtcttcc tgataccacg gctcaatcag gttgttggtc
     9601 gcatccatca ccagtgcgtc gaggctggca taatcaccgc ccttacccac gcggatgacc
     9661 tcagaggtgg tgcgaccttc ctcgtcagtg accttgctca tcacgcgcgc cggggcttca
     9721 ttgcggtatt tctgcagcca gccgaccgcc acatcctgca acatcggatt gctgctgcgg
     9781 tcagaggttt cggcacgctt cacgccgtta aaaccggcca tgatgaaatc aagggactgg
     9841 cgtttgataa tggcgttacg gacacggagc tggaaatcct gataacgcgc ccacaggtca
     9901 agcgttttgt agcggatata aaaatcgaag ttaatctggt cgcattcgta cttgtttgac
     9961 gccagcttcg agaagtcctt cggctgacgc tcggtgccac cggcggtgtc ggtggtgctg
    10021 gcgatggagc cggtgacacc aataccaatt ttttcccctt tcatttcgct gaccggcaca
    10081 atgttgatgc gggtcagaaa gtcagaggac tcctgcatgg tgttcatcag ggtctgggtg
    10141 accgaaggtt caacggtgaa ttttttcgac acatcaccgg cgtcgatgcc gttcagttcg
    10201 gcaacacggg acaggtaggc attaaattta aagcgggttt cctggcgcat agttattcct
    10261 gaaattaagg gttaatcgtg aaggttttcc cggactgacg ccggtcagca gttcgtcatc
    10321 agggcgtcac cgccaccgcc ggtggccttg ctgcggcgct gctgggtcag actttcggtg
    10381 tggtcgagac tgtttttcag gcgggtgaat gcctggctgg tttcatccgc cctgtcagtc
    10441 acatcctgct taagtgcgga aaaggcgttt tccatctcag cgaggcgctg ctcagtggcg
    10501 ctcagttttt cctgcacatg ttcagcaaca gcggtcaccg cttcatgcac gtcattcaga
    10561 cgggcgtcat cgctggcctg tttgcggcca aaaatggact tcaccttttc ggtcagggcg
    10621 gtgaacacgg tttcaggcag gtcttcaaat tccagctcaa caggcgttgc cactgaaatc
    10681 aggttttcag ggcttaattt gaagcggttc agggggttgt gttttgccgt gcggcagaat
    10741 tccaggtatt ccgtgccgag gcttgccggg tcatcggtga cggccagacc caccagataa
    10801 catttgccgg tattggcaaa gttcggctga atttccattg aggtatagac cttctgcgcg
    10861 gccttgttca tcgcgataag gtcatcggtc ggggtgattt tcgcaaacag cgcccatttg
    10921 cctttcagcg ccgaatcatc gtcaatcttt tcggccttca gttcgaccac atcgccataa
    10981 cgcttaaaaa taccgtcagg caggatgccg cgcagatgtt ccaggttaat gcggcaacca
    11041 tagactcgcg ggtcaaaggt ttcggccatt tcctgaatat cctgcgcact gatgacacgc
    11101 ccgtcacagg tgtcaccctc aacgccgata cgaaagaatt ttgagacttt ttttgccatt
    11161 gtcaggagtc ctgaatagtg attagaggag tcacatgtcg gcatcagttt cccgacgatg
    11221 cgcatcctcc gccatcagtc ccggatggct tatcactgac acaacagcac cttagcgaat
    11281 cgcggggcgc gactcagtag ccttgccgtg tattcatcac ggcgaggtat tcatgaccat
    11341 caccacagac accactcttt tacacgaccc gcgtcgtcag gcggcgctgc tgtactggca
    11401 ggggttttcc gtgccgcaga ttgccgccat gttgcagatg aaacgcccga cagtgcagag
    11461 ctggaaacag cgcgacggct gggacagcgt tgcccccatc agccgtgtcg aaatgagtct
    11521 ggaagcgcgg ctgacccagc tcatcatcaa accgcagaaa accggcggtg acttcaagga
    11581 aattgacctg ctgggacgcc agattgaacg actggcacgg gtaaaccgtt acagccagac
    11641 cggcaacgag gcagacctta atccgaacgt cgctaaccgc aacaaaggcg ggcgtcgcaa
    11701 accgaaaaag aattttttca gtgacgaggc catcgaaaag ctggagcaga ttttctttga
    11761 gcagtctttc gactatcagt tgcactggta tcgcgccggg cttgagcacc gcatccgcga
    11821 tatcctgaaa tcccgccaga ttggcgcgac gttttatttt tcccgcgagg cgctgctgcg
    11881 tgccctgaaa accggtcata accagatttt tctgtcggcc agtaaaacgc aggcgtatgt
    11941 gttccgcgaa tacatcatcg cctttgcccg gctggttgac gttgacctga ccggtgaccc
    12001 gattgtcctg ggcaataacg gcgcaaaact gatttttctc ggcaccaact ccaacaccgc
    12061 acaaagccat aacggcgacc tgtacgtcga cgagattttc tggataccga attttcaggt
    12121 actgcgtaag gtggcatcag gtatggcctc acagagtcac ctgcgctcga cctatttctc
    12181 caccccgtcc acgctggcgc acgacgccta cccgttctgg tccggtgagc tgtttaaccg
    12241 gggacgcgcc agcgccgctg aacgcgtgga aatcgacgtc agtcataacg cccttgccgg
    12301 tgggcttctc tgtgcggacg gccagtggcg gcagattgtc accattgagg acgcgctgaa
    12361 aggcggctgc acgctgttcg acattgagca gctcaaacgc gaaaacagcg ccgacgattt
    12421 taaaaacctg ttcatgtgtg aatttgttga cgacaaggcg tcggtgttcc cgttcgagga
    12481 gctgcaacgc tgcatggtcg acacactgga agaatgggaa gactatgcac cctttgccgc
    12541 aaatccgttc ggctcccgcc cggtatggat tggttacgac ccgtcacacc gtggcgacag
    12601 cgccggatgc gtggtactgg caccgccggt ggtggccggt ggcaaattca gaatacttga
    12661 gcgtcaccag tggaaaggca tggactttgc cactcaggcg gaatccatcc gcaaactcac
    12721 cgaaaaatac aacgtcgaat acatcggaat tgatgccacc ggcctcggtg tcggcgtgtt
    12781 ccagctcgtg cgctcgttct atcccgccgc gcgcgatatc cgctacacgc cggaaatgaa
    12841 aaccgcaatg gtgctcaagg ccaaagacgt catccgccgt ggctgtctgg aatatgacgt
    12901 cagcgccacc gacatcacca gctcgttcat ggctatccgc aagaccatga ccagcagcgg
    12961 acgcagcgcc acctatgagg ccagccgcag cgaggaagcc agccacgccg acctcgcctg
    13021 ggcaaccatg cacgccctgt taaatgagcc actcaccgcc ggtatcagca ctccgctgac
    13081 atccaccatt ctggagtttt actgatgagc aagaaaaaag ggaaaacacc gcgacctgcg
    13141 gcaaaaacaa tcaccgccag cgccccgaaa atggaagcat tcacctttgg tgagccggtg
    13201 ccggtactcg accgccgtga cattctggat tacgtcgagt gcatcagtaa cggcagatgg
    13261 tatgagccac cggtcagctt taccggtctg gcaaaaagcc tgcgtgctgc cgtgcatcac
    13321 agctcaccga tttacgtcaa acgtaatatt ctggcctcga catttatccc gcatccgtgg
    13381 ctttcccagc aggatttcag ccgctttgtg ctggattttc tggtcttcgg taatgcgttt
    13441 ctggaaaagc gatacagcac caccggtaag gtcatcagac tggaaacctc accggcaaaa
    13501 tatacccgcc gtggtgtgga ggaggatgtt tactggtggg tgccgtcctt caacgagccg
    13561 acagccttcg cgaccggctc cgtgtttcac ctgctggagc cggatattaa tcaggagctg
    13621 tacggcctgc cggaatatct cagcgccctt aactctgcct ggctgaatga gtcggccacg
    13681 ctgttccgcc gcaagtatta cgaaaacggc gcacatgccg gatacatcat gtacgtcacc
    13741 gacgcattgc aggatcgcga ttacattgac tcaatccgcg aaaacatggc taattcgaaa
    13801 ggccgcaata actttaaaaa tctgtttctc tatgcaccgc agggaaagcc agacggcatc
    13861 aaaattatcc cgctcagtga agtggcaacg aaggacgatt tttttaatat caaaaaagcc
    13921 agcgccgctg acctgctgga cgcgcaccgc atcccctttc agttgatggg cggcaagccg
    13981 gagaacgtcg ggtcgctggg tgatattgag aaagtggcaa aggtctttgt ccgcaatgag
    14041 cttatcccgt tacaggacag gattcgggaa ataaacggct ggctcggcca ggaggtcatc
    14101 cgctttaaaa actactcact ggacactgac aacggctgaa catcgccgcc tgcgggcggc
    14161 ttttttacac cccgtcatca cgccctcaca cgctcaccac cgcacaaaac accccgcaga
    14221 cacaccaacg ccccggcgca cactctaaac gccatcacga cgcgctcaga cgctgaaaaa
    14281 ataaaatcag caccaccgcc agcgcgcagt gctttccccg cctcgcccgc ccgcttcatg
    14341 gggcggtttt aatgcagatg cacgaacacg ccgaaggcgc gccagtaccg gcggcgctca
    14401 cacgtgactg ggaaggaaaa cgcatgcgat tcgatgcagt aatgcatgca gagctaaaaa
    14461 tggcagaaca tactgaaaaa acgcataaaa aaaccggcat tcaggttgcc ggtttgagtc
    14521 gacttcattg tggttactgg ccgcgcaatg cgccaataat actggtaagg caacaagtgg
    14581 caacaatcag caaaaaaacc gtcgtccacg gattttcata aatgagagat aacatattga
    14641 taaagatcct tttatttgtt cattctgatg tggtttaaaa ggtgtttgat aacgaccgta
    14701 agctcatctt tactggcttg ctcgaccatt ttttcggtgt agctttccac ttcgcgggaa
    14761 ctcaggtcgt tatttgaggc cattacagtg agtctctttg cccagtcggc ataagggtct
    14821 ttaattgatg aaagggaatt atgcatgtcc aaaaacctcg aattatttaa ccagcagacg
    14881 gctgaaatct ttgcggtgct atgggataac tttcctgtac cacaagtcat cacctacaaa
    14941 aaatttaacg ccgcgttacc tgatgactac tttgaccaac ttaactcacc agaaatgaaa
    15001 gcactgaatc agttgcgtag tgtggttgat ggcacattca ctttcctaag cgaaaacggc
    15061 tatattctgt acggaacaga ccatcagacc ggttttcatg atgtgcgcct gaccgagaaa
    15121 gcactcgcgg tgctcaacaa aaaacccgaa gcacttggcg gtaatgaaac gatgggagat
    15181 aaaattatca gtgcagtaaa agacgggact ccaggtgtca ttgctggagc agtgacaaac
    15241 ctgttgactc ttggtgttaa ttttgtgacg tcagcttcat agctaactac tttctgaggt
    15301 ttctaatcaa cgccgcctgc accgaaagta aattcctgca ctggcggcgt tactaacgcc
    15361 aaagttattt taattattga tgctattatg caatacattc aatatgttaa tttgcagtat
    15421 tagcctttaa agtaagcacc ttagatttgt aacgttgctc aatcactccc attgaatgca
    15481 aacgaccaaa gagctttttg gcaatgattg tttctctcgc attttcggcg tctttaatta
    15541 ttttccaata atctatttcc ctagccatca aaaaatgctg ctcattccta acttttaatg
    15601 cattgcctaa tacaataact ccagttacca acttaggctt atctctggca tatagttttg
    15661 ttttcgttgg gtgtataaca tgcccatgct tattaacgat cttacgaatt acagcgcaga
    15721 acaatcgatt tacgttacta cctgaaaaag tgagatcatc aacataaaca gtcatgttaa
    15781 ctcgtaactt ttggcaaagt tgatatattt caccaaacat tcttgaattt gcaaaatatg
    15841 ccaacggcat actgatgcga ctacctgttg gcaatcggtc atgacaagta catatgtgag
    15901 acaaaacatc agcaacatca gatgacattt tcatcaccga aaagaaaaaa gaaaatatca
    15961 tccctcttgt agttgatgga aaaaaagctt taatatctgt tgtcatcatc ttttcattat
    16021 taagatgagc tttagcattc gtcacattag agcacttctt cttccctgag tgcagatatt
    16081 ctggcaaagc aatccgcgat aacaaacttg caattcgtgt atgtacaagt tctagttttt
    16141 ctaaaggctt ttgtattttt cgtgctttcc cttttttaga aagctgctca aaaacggaat
    16201 agtttccttc atcttttctc aaagagtata aatcatcaag actaacacaa agcaaattcg
    16261 ccagttttct tttacttttg agtttataaa atggcgaatc cattatgtca taagatttat
    16321 ttttagtatt aactttaagc tttttccgtt ttttcatttt tattacttac ccactccaga
    16381 acatccagaa ctttaccggc taaatttaaa cgcaatcgct tagacaaacg gccttgcgtg
    16441 cctaatgatt ccgaaaagaa tactaaagag gatactggta tgtcgaaatg ggaggcgtaa
    16501 cgctctaaga cttcaatcga cggtgaccaa actccgcttt caagcgatac tattttgtct
    16561 tttggaatac ctaaagatga agataactca actattgatt ctttatggta ttgccttatt
    16621 aaacgaaggg ctttatgtaa cataggcaac tccatgctat tgaaactagg gaggacgcat
    16681 tactcatcac ccccttcgaa gtagtccagt gctcgagtaa ctaaatcaaa cagtctaata
    16741 ccccatttga ttaatttcca agcgtaacca gcccactttt tcaggtttga cttgttctgc
    16801 gcctgctgtt cgttggttaa tttatcattc ttcatgattt gctccttatg cccatagtgt
    16861 gcaaacggta tgtctgctcc aacactatgg ttcgccctca taaggatgag attgaacagc
    16921 actagccccc tgctccacga acccttggtc gcccaaaggg ttcgaatacc catacgccat
    16981 acctcctgat cctgtgactc ctgtcgctgg caggggcgtt gcgcggggta agtgttagaa
    17041 agcgttaact ttccacgggt aaaaataccc aattgatggc gatatatctc actaactaac
    17101 caatgtttgc atcataacaa gtaaaccgtt gtatttgctc cccctgtata tgatattttt
    17161 tcatataaaa caaatatata ataagttaaa ggcatatcaa tgcaacatgg atatgctttc
    17221 attgcattga attttaaagt gttttacatg aaaaatgact cttcatctcg aaatccgaac
    17281 cactcattaa taaccatata cgtgaatttt ttcccgtcat aatttacggt tgccccacgc
    17341 gccagcgcct caatctccca tcgctgaggc ctgataccgt tctgagcaag gtcaacgcgg
    17401 atacgggtaa tttgcattcg ttccgaccgg gtcagtctgg ccgatggcgc tatttcatgc
    17461 ggttttaacg ggcttccgct tctttgctga cggtttggtg ttctcaggtc gtgttttaat
    17521 gcgcccctga gcgccctcac gacctccggg tcattccatt cgataacacc gtcatcaacc
    17581 agattaagca ctgcggcggc gtgctcagaa ggtgtgggag ccggtaacga agtatcacca
    17641 ccggtgagct ttccacagtt attgacagga ctccgaggcg cggcgatgcc gctttttaaa
    17701 gtcaaaggct ccacgaccgg aactttcggc acaatgcgcc agtccgtcgt tctggtgata
    17761 tgaatatgac gcgcgccgag atgcggcgcg taaatgccga ccactctctc gacttcttcc
    17821 tcgtactcgt taacgtcatc cgacgggcta cgggcgactc tgacagtctg gcaatcgcgc
    17881 ggaacatttg ccccaccctg cgcgctgata tacaacgcaa aatcgccact gtctgcggca
    17941 gcacgtgcag cctcgacgcg ttcgtcaaac tcatcagcaa tgctgacgcc gcgaggcaat
    18001 ttgcgtagtt cacggtaagc ccccattgtc ggcaggccaa ccgttttaaa ttgcgggata
    18061 cgccacgttg acgcccatgc ggtaacagcc gctgcagtgt ctttcagcgg cctgccggta
    18121 tcgttatcga gctgaccatc cagtgcatag ccgtcgatgt tttttgaaat gtatttcgcg
    18181 atatatcccg ctgcaccgcc ccggttaagg tgttttgcct gaaaacggtt tcgcgcagct
    18241 cctctttcat cgccatcctc tttgagcgca tagcgacgca tgatttcgat aatctggtta
    18301 cgctggcgtg gattacaaaa aagcatcata tgccagtgcg gtgttccatc gtggtgtggc
    18361 tcgacgactc gcaaaccgta gacctgtaaa tcattatcct tgaatgccgt gcgcatcagg
    18421 ctccagatac ggcagagata acgctgcgca tcctttggat taaatgcctc atcgttccag
    18481 ccgtgattta gctggacggt tttactttcg ccttttccga cctgacgtgt cgggtgatac
    18541 tttgacggcg tggtcagcgt gataaacatc cccacatcac cctctgcggc ggcgtaacgc
    18601 tcaataccgg caatggtgtt catcagctcc atccggcgaa tttcaggatt agaaatactg
    18661 cccatcacct tactgataag gtcgatgcgt tcgccggttt ccctgttttc gaggtcacac
    18721 gatttaagaa attccagatt tgcctggcgg cgcgcacgca catcacgaat ggcgtgttta
    18781 ctggcataag gagaacggtc tttattgacc tccccgacag caatcagtaa cgcctcatgc
    18841 cagcgcatac gctggccttt aagctgatga gtccaccact catcgttaaa caggcgggca
    18901 atggcagaat atgcctgcct cgtggtcatc tgccctttac ggtatttttt ccagtaaagc
    18961 ggggaaatat tgaaagcacg tgcagcgcca gcaacatgac catacaggtg agcctgcgcc
    19021 tcatccgtaa acagcgattc tttttcgcca tgcgcatcca cccaggcatc gcagatttcc
    19081 tcatacatca tgaaaagctg cgatgagata cgggcggcaa actttttcag ctccttgtca
    19141 ttcattccag gcaggcgcgc atagtggtca cgctctgcca gaaacagcaa cgacgcgtcg
    19201 gtgttcattt catggcgctg attcacgcgc tcaatgcgcg gccataaacg acgctgaaaa
    19261 gtggatgtga ggaaataaaa cccgtgcacc gggcttttat tgcgccggat atagtcatag
    19321 cgtgaagtaa acagcgagcg caaaaagtaa ggcaggcggt taatcgtgga taaaacacct
    19381 tgcacctgac gcatctcgtc acgtgtaagg ggtctttcgc gcccgacagc ctcacgtggc
    19441 gcgttccatg cataagcacc ggtaaacgtc ttaccggtgc ctgcggcaaa tgctgacgaa
    19501 gggacaaaat gcccggaggc tttaacggcc atgtgtgcca aaagcctctg aacaacgctt
    19561 gctgagttgc tcaacctgcg cgtttaaatc ggcaaaagat tttgcgcttc cggtcagaat
    19621 atcgtgatgc atcaggccgg aaacgagctg gcttaatttc gggtaataac caaccaccgc
    19681 cagccattcc tgaccggcgt ttttaccgct ttccgctctc tttttctcgt ggagaataaa
    19741 ctgaaagctg tcactggtaa cgacataacg ttcgccaatt tcaatacgaa tactcatgcc
    19801 gttctccggt aatgtttgtt ttttgcttca aagactgact ggcaggaaac acaacgcgtg
    19861 gctgacggat aagccgcacg acgggcagca ggtattggcg catcacactc ttcgcaaacc
    19921 agcgcagaag cacagcaatg ttttaccctt gccgcgttaa tctggcgctc cagtaattca
    19981 gcctgttgtt cctgaatgaa atctacgttg tccggcatta ccagttcctt ttgtcgttca
    20041 gtttcttaaa ttcatcagcg caatagctgg cgagttctgt cgttaattta gtcagttcat
    20101 ccacggagga gatttgcttg tggaatacag cgcgtttaac aagtaaattg accacatcag
    20161 acaggaggtt taattcattc tgataaatcg cgataacaga ttcagttatg tcgcgctttt
    20221 ctttatcaag accaagttga ataagagaca aatcgccatt ttccataacg gcgattttta
    20281 aggcgttatt cagtaataca attgaacgag aacaggacat caaagcacct ccccgcgaga
    20341 caatccgata ttgtgaaatt tttccgactc ctgactgagc agctcgacta tctccacgcg
    20401 ggataactcc gcctttgtga tgtggcgaat catggcgtca agatgagaag aaaagcgcgt
    20461 cgcagcgtcg gcctgtgctt cggttctggc ctgttgcagc agtaatgcgt atataccgca
    20521 ctgattttca gaaactgtat gcatgacttt ctccaggcaa aaagaagccc cgcacgatta
    20581 agtgcgttaa aaactctggt taattactta atgcagatat tgctctggtt ttaccgacgt
    20641 cagaattgtc ggtgcatact caaacagact gaataattca cgtaatgcac ggaataaagc
    20701 atcacgccag taacatgatt cttcattaat tcgccagtat ggctggttaa attctttttc
    20761 tgtcagtcgt gcgtgcataa ataaagtgcg acgctgactg actgttaaaa aactaatata
    20821 tgcatactca cttgcgccaa cctgacggcg ttttgagaat gccccacgca attcatcaat
    20881 tgcacatacc agtcgttcac gttcgacgtc gttcatttct tcaaaacgca tcgttgcgtg
    20941 acgctgtttt aactgcgcat ggaagcaaac cgttagccgt tcacgttcca tcatctgatt
    21001 ataataatcg catgtctcct gccagcgagg gacggccaga tgcttaccaa ttatccggcg
    21061 catagctgct ggctgttttt caacgagatt gagcgtcatc actgtcattt ccataccctc
    21121 cggcttttca gaaaggtcag agcctttttt aacggactct gttttttggt acggataatg
    21181 attcccttac gccccttacc gtgggtgatg gtgaagtcaa tcgccctgga gctttcgtta
    21241 cgcagtaact gagcaataca acgaggctca ttcatacggt tctccttaac gtggttcacc
    21301 gagacctaac cacatcaacc agccgtcacg aatctcttta gggcggcttt cataagccag
    21361 ttttagtccg ttattccatg ccggaaggta tacccaatat tcacctgcac gacctgaagc
    21421 tgattgtgga tcggtcatat caattacagg cagctttcct ttatcgatca tccgacgaac
    21481 cgctcctgtc gattttccta ttagttttgc gaactcctga taaggaatcg catcagtcat
    21541 gagtgttact tgcttgctca tgtcgtcctc tagccctcat gaattgcgtt taatgtctta
    21601 taatgccttt tagtgcccac atccaagcac taaacaatct acatctaaac taaatactat
    21661 tgagatctaa acactatgtc aaacacgata agcgagaaga tagtcttaat gcgaaaatca
    21721 gagtatttga gcagacaaca acttgctgat ttaacagggg ttccgtatgg cacgctgagt
    21781 tactatgaaa gtggtcgttc aacacctcca acagatgtca tgatgaacat cctgcagacc
    21841 ccacaattca ccaaatacac tttatggttc atgaccaatc agatcgctcc tgagtccggg
    21901 caaattgcgc ccgctctcgc acactttggg caaaacgaaa caacgtcgcc ccactccggt
    21961 caaaagactg gttaacaatt catcgtgaat atattcatca caagtgccta ctattggtgg
    22021 ctaaatttca gccaccacga aaaaagcgat tagtagtcgc aaaaaaacac accactcgga
    22081 gggttttctg atggcaatca aaaaactcga tgatggtcga tatgaagtgg acatccgccc
    22141 tactggacgt aatggaaaac gcatccgtag gaagtttgat aagaaaagcg aagctgtcgc
    22201 tttcgagaaa tacacgttgt acaaccacca caataaagaa tggctatcaa aaccaacaga
    22261 caagcgacgt ctgtcggagc tgacacagat ctggtgggat ttaaagggta aacacgaaga
    22321 gcatgggaaa tctaatcttg gaaaaattga aatcttcaca aaaataacga atgacccatg
    22381 cgcatttcaa attacgaaat cccttatcag ccagtactgc gccacccgaa gaagtcaggg
    22441 tattaaacct tcgagtatca atcgtgattt aacatgtatt agcggcatgt ttacagccct
    22501 gattgaagcg gagttattct ttggtgagca ccctatcaga gggacaaaga ggcttaagga
    22561 ggaaaaacca gaaacaggct atctcacaca ggaagaaatt gccttactgc ttgcagcact
    22621 tgacggcgac aataaaaaga ttgcgattct ttgcctgagt acaggagcac gttggggaga
    22681 agcagctcgt ttgaaagcag aaaatatcat ccataaccgc gtcacgtttg ttaaaacgaa
    22741 aacaaacaaa ccacgcaccg tcccgatctc agaggctgtt gccaaaatga tcgcggataa
    22801 caaacgaggt tttttattcc ctgatgctga ttaccctcgc ttcagacgaa caatgaaagc
    22861 aataaaaccg gatttgccaa cggggcaagc cacacatgca ctaaggcaca gctttgccac
    22921 tcatttcatg attaatggag gaagtattat cacgctacaa cggatactag gtcacacgcg
    22981 gattgagcaa actatggttt acgctcattt tgcgccagag taccttcagg acgccatttc
    23041 tcttaatccg ctaagaggtg gtactgaggc cgagagtgtc cacacagtgt ccacagtaga
    23101 gtaacgttta agggctttca gtggtaattt atgccgctca aacccgcatt gtaccgttga
    23161 aagcccctac tggtgacacc ctaaatctcc cttacacggg cttatttttt atgcataagc
    23221 cctatccctg ataaccgtct tccattgacc acatcgatag aatctccctt catagcacga
    23281 tgcctttcac gtaacggcat cgtgctcgca caggttccgg ctaagcacaa ccagaacgcg
    23341 catgtttgac gcttaccaaa aaatattctc actctccaca tttgaatgtc agacgagcga
    23401 cgccatgtaa tcctgcacct tctgtcttca ggtcaactat ctgcattttt ttgccctgag
    23461 taacacagaa atgggctgca tcatttttta ctatattttc tgcaccagat attctgcccc
    23521 tggctaaaga agcttcggct tcggtgtagt attggttatc gagtttacgc tgaatattac
    23581 ttttatatgc aagaccaaat ttaccgatac ttgtctcatc attatgcaca gcacaaccag
    23641 acataagaaa aacactaatt aatgatatag cagctatctt tttcatctca ccttccccca
    23701 ttaaatacca acgacactct ctagtgttta aatataataa tggcatgatt attataattg
    23761 aataggatta taataaatgt tctgtaaaac atttcctaca taagtaggaa ttacggacat
    23821 ggaggccctt ccgggtaact ccatggaggc tttaatatat tatattgaag aagccactgt
    23881 ttagttgaat attaggtata tgctcttttt tgaaatttat cggtggctgc cgttagtatt
    23941 cgctgtcccc attgcaagct cctggtggta accactgaat cctccatact tgaactgact
    24001 atttatcctc cgactttcat cctgttctga ctccaccttt tcttttctgc tctacactat
    24061 ctatagacca atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat
    24121 tcttaatggc aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga
    24181 ggaaggaatg acgatccatg tgcgggtcac ctgggagaaa ggcgatgccg cacgatatgt
    24241 agaggaggcc cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat
    24301 taatgaagtt tctacggcgt tgattcagtg tgagggggat gacatacccg cgctgggaat
    24361 tttgccatta ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga
    24421 taaggcgctg aaactggcaa ttgccggtaa cgccattgcg atagatatgg cgcaggtcaa
    24481 caaacaaacc tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga
    24541 aacgccggaa aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat
    24601 gcgcatggat actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca
    24661 aggtgacgcc ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt
    24721 gtgcccgaac gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga
    24781 aattcttccg gctctcgtat caaccttaaa atctgacgaa gataacccga atattatcga
    24841 aggcgcttcg tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg
    24901 cgaaccgttg agtgggcaaa acttccatat tgaaatactt ccggcggcgt tgcgttgtcg
    24961 attaccacca gattgtccat tattgcgtta atcaaactat tctctcttga accagggaga
    25021 atccgtaatg ccggatgcgg tgtaaacacc ttatccggca tacagaacaa tactacaccg
    25081 taattaattt tacccgtgtc ttctgcaatg ccagtttatc cacatctgac acggcatcat
    25141 cggtaataat aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacgattaa
    25201 atttcgatga atcgagaacg gcaatcactt tatttgcggc ggtgaccatc gccccgctaa
    25261 tcgaataacc ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga
    25321 caaacattaa atcggcatta atatcctgca atgaacgctc ggcaatggaa ccatgcatcg
    25381 agcgcgtttt atggcggacc gtgccaccac agacgaccag agtaatgtct ttattttcgg
    25441 aaagggcaaa cgctgccggg agactgttgg taatcaccgt gatatttttg gcggtcatta
    25501 atccttcggc gataagcatt gtcgtacttc cgctgtcgag aataacggtc atcccttcgt
    25561 ggatcatttt gactgccgcc tgcgctattc tgttttttgg cgcgctggca agctgaaatc
    25621 gctctttaaa cccgacttcc tgggtctcgg tttcactatt accagacatt attttcgccg
    25681 caccgccatg aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga
    25741 ttgtcgcttc cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt
    25801 cattcactaa ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat
    25861 attcaattgt ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa
    25921 taccgggcca gcatcacgct ggcccgcggt ttcagggaat gagcaacact ttgcccggca
    25981 tagcattacg agcgatgtca cgcaccgcct gggcgaagct ttcaaagctt ccacggtgag
    26041 cgattaatgg ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact
    26101 cctgccccgg ccaagggctg gagtagttca tccaactgcc gataaccgtc agctctttac
    26161 gcaatatttt gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac gtgcccacca
    26221 gtgccagttg ggcatgagga ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc
    26281 cagccgtctc gaggataagc tgattaaagc gcagttcgcg taaaacgctc tgcatttgcg
    26341 gcgcgctcat ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca
    26401 gtttttctga gctaatgtcg atagccgtca cactctttgc tcccagcgcg acagcgcact
    26461 gaatagccag caggccaatg gttccggcac caataataat aacgttttta ttctcacaac
    26521 cttgcgctaa atgaaaagca tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct
    26581 caataggcat atccgtgggt agagcaaaga cattttttcg cttaacgaca atatattcgg
    26641 caaatccacc atcacgccgt gagccaataa aatcatattt tgcgcactgg gaataaaacc
    26701 ctttcaaaca ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc
    26761 cagggtgtaa atcatcaaca ccggatccta ccgcatcaat atagccgcta aattcatggc
    26821 ctaacgttat tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac
    26881 ataagcccga gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga
    26941 cgctttctgc aacgcgcacg ataccatcag tatcattcac cactgatttc ataaaaactc
    27001 ctaattgtta agggggataa cctccccctc aggtaaaaat tattctgcga gaacgacttt
    27061 ctcttgttta ataaagccac gcgctctacg ccaggtcatg aaaataccgg taagataaat
    27121 tgcgccgata ataatgaaac cgggaatatt ttgcggggag aaaacctgaa tcagtaacca
    27181 ggtaatggga gaaccgccct gatccattga agccaccata cccccggctt ttaacgcccc
    27241 agcattagcc gccagttggg tgtgcaaacc aatagtttgc gtcgcgatcc acagggtgat
    27301 gctcataatg atgacacccg agattaaggt gcggaacaga tttccacgat gcaccgcgac
    27361 cgccatcgcc acgaagaagc cgatagtggc aagatcgcca aacggcagta cctgattacc
    27421 aggaacacaa acagcaatta aaatggtgag cggaataaaa atcaggcttg ccgataccac
    27481 cgccgtatgc cccagcagta atgcgggatc caggccaatc aggaactcct gaccgccgaa
    27541 cttcgcctgt aaacgactac gggcctgctt agcgatgggc gttaaaccat ccatgatggg
    27601 tttaatcacc cgtggcatta gcagcatcac cgccgccgtt tttaccgcca gctgcaatac
    27661 acctttgaca tcgtaacccg cgaggatgcc gataatcagc cccatcacaa aacccacggt
    27721 gacaggctcg ccaaacgggc caaatttgcg ctgaatatcg tcggcgctaa atttaatacg
    27781 gttaacgcct gggattttct cgatgatagc atcgaccagc accgcaatcg gccccatata
    27841 cgccgacgta ccgtgcggaa tagcaatgcc ttccagctca aagaaatttc gggtatcgcg
    27901 ggcaaaccag tcgccgagct tataaacaaa cgccgcgtga attaccacac ctgccatccc
    27961 tatcatccat gaaccggttg ccagatgcag caacgcgccg gtgaaggtca tatgccagat
    28021 attccagata tcaacattta ccacccgcgt catacgggtc agtaacatcg ccacgttaac
    28081 cagaatcgca atcggaatcg ccaccagcgc aacttgcgac gcccaggtca ttggtgaaga
    28141 gcccggccag ccgacatcga ccacatgcag attcaggtcg aaattttccg ccatcgcttt
    28201 cgccgccggg ccaatggaat ccagcattaa gccaatcaca aggccaatgc caacaaagcc
    28261 aatcccgata tgcagacccg ctttaaagca atcgcctgcc ttcatgccta aaattttaga
    28321 aaaaataatg atgacaatcg gcaacatcac cgtagggccg agatcgagaa tataacgcat
    28381 gacttctgaa aacataggtc acccctgtaa gatagtcaga attttatttt gtaatgcttc
    28441 gataccgaca ccagaaacaa aaggcatgcc gtgaactaac ggaatatcgc caaaactacg
    28501 atccaccctg gcagtggtgc atatcaaatg cacgccatcc atataggttt ctatttcatt
    28561 aacccgacac tggattaatt caacaggaat attatgactc tgacacaact ctttaatttc
    28621 ttccgccgcc atcgtagagg tcgcaaccgc gcctccgcaa gcgacaataa tcttgcgttt
    28681 cataaatagt cctttttatt gttacagagg gatttattcg tctgaatcta aaacatactt
    28741 tgtgaagtat tcctttaact gagtttcagg aagagtgatt agtgtctcaa cgatatcggg
    28801 ctgttgtaac ttgccaaata aacagcgtaa aagtttcaat tgctgctgcg gattttccac
    28861 aatcaacgca ataaccaacg ataccgccac gtcattatca tcatccgctt gctgaaaatg
    28921 aactttattt gttggcctta acagataaat ggctgacgac ttagcatgaa tcgcctcaca
    28981 atgcggtatt gcaatggcgt gctgctcaag cattatcccg gtagggaatt ctgcttctct
    29041 ggcaattaat gcctgtggcc aggtatcatg aaccacacct ttggcgagca tctcattacc
    29101 gatatgggtt aaaacttcgc tacgatcgac aaaagaaatt ccgctacgaa caaacaggtt
    29161 agtcatatac cgtccttatt ccgcacagcc gtagcgatag gcgcgcaaaa catcataaat
    29221 tttatccata atcagctgat gcggtattgc tgataattcc ccggactgaa tgcgttcaaa
    29281 ttgtttggga agatactgac taatcatgcc cagtgggatg tccacgcctt gcagattcac
    29341 catcatcgtt tcaacgctat ttttaatccg actatgcggc cagtaataac gaatacgatc
    29401 cgacaggctg taacgaatat ccagtaatga atcgttaaaa cccgtgcgat aatatttttt
    29461 ccagtattgc ggttcgtcga gcatcacttc ttcaattacc gccaggcaac cgctgcgatt
    29521 ttcgggggcg ataagttcct gctcaatttg cgccagcgca aatatcgcct cacgtaaagc
    29581 aaaggttaat gcgggaccga ctttcaatat tgcaaagtga tcgcggacta attcccaata
    29641 agccgtccgg gtctggtaat cggtagaatg tgcttcataa accattcggg tattttctat
    29701 ccattgcgcc agcgcctgcg cttcctgcgg ctgataatgg ataatattgc tgtgatcaaa
    29761 ttccacaccc ggctgcacca cgatggcaat cacgcgtgtt aatgcctctg tcagcccacg
    29821 ggcaataaag gccttttgat gcgtacgtaa agtattggcg gcatcttcaa catgggtgat
    29881 gtgtactgac tgaatggcgc tggcctcacc gcccggaacc ggaacttcgg tgccaatgac
    29941 atagctcagt tgctcacgct ggcaatctgt cgccacactt tccgcagcaa agcaaagcac
    30001 agcagctcgt tccgcaaccg tttctggtgc taacggtatg ggatcccccg cgcaggacat
    30061 tgacgcatca agatgaattt tactgaagcc agcacgaaca tatgccttta ccagctcgac
    30121 ggatttttcc atcgccgcat ccacattttc ttgctgccag cagtttggcc ccagatggtc
    30181 accgccgaga ataatacgct cgcgtgcaaa tccgacttta tcggcaatcg caaaaacaaa
    30241 ttcgcgaaag tctgccggtg tcattccggt ataaccgcca aattgattga cctggtttga
    30301 cgttgcttca atcagcactt tgcgcgtgct gttgcgatca aatgccagcg ccgcttcgat
    30361 aaccaacgga tgggcagaac agactgaaca tatgccgata tgttcaccag ctttatgccg
    30421 ggcaattaac gttttcatgt tttttccttg ttaaatggca ggtgcgttat gccctgccct
    30481 cgcagccaca atcggcaatc actttgctca ccacatcgcg cattgcggat ttagccgact
    30541 gcaaataatc ccggggatcg gtcgcttcgg ggtgctcggt caggtaattt tttaacgcct
    30601 gcgagaaggc atttttcagc tccgttgcaa cgttgatttt gcatatcccc agtttgatgg
    30661 tttgctgaat atccttagtc gataaccctg acgcgccatg cagcaccagc ggtaagttca
    30721 cccactggcg aatgttctcc agtcgagaaa aatcaagcgc cggtgcgctg gcatacatac
    30781 catgagccgt gccgatggcg accgccaggg aatcaattcc ggttgcctcg gcaaattcac
    30841 gcgcctgagc ggggttggta taaaaagcat cggcttcatt gacttgcaca tcatcttcct
    30901 ggccgccaag ttgccccagc tccgcttcga cgctgacatc aaagcgatgg caaaaatcca
    30961 ccacctcttt gacccgtgaa atattttgcg caaaaggcaa atgcgaggcg tcaatcatga
    31021 ctgagcgcac gccagaacga accttctgag cgatatcgtc aaatttcgtg tgatggtcga
    31081 gatgaatcgc cagtggatgg tgataatgct tcgccatcgc gctgaccagc gccaacagat
    31141 tttctgtacc agcatgagta aatgtgccag gcgttccggc gatgatgacc ggcgcatgca
    31201 ggttggcagc ggtttctacc accacttgca tcgtttcgag attgtgaata ttgaatgccg
    31261 gaaccgcgta accgccgcgc tgcgcgttgt tcagcatctg ctttgtcgat accacgtaca
    31321 ttttcacatc ctgtcgattg ctttcgattt caaaacataa tgaaatcact tgtttttaaa
    31381 tgtcgagata acgatcacaa aaacgacaat atgaaaatta atagaggaat gaaaggcaaa
    31441 aaaaacggcc tcccggaggg aagccgcaac aaagtgcgcg tggttttatg caggatgcgg
    31501 cgtaaacata tgtggcagcg ctgcgtaggc atgataagac gcttcagcgt cgcatcaggc
    31561 ataggttgcc ggatgcggcg taaacgcctt atccgtccta cggggtagtg ccgttcgtat
    31621 caggcgatag taattttgct atcgagataa acgtcctgca cggcgttaat cagtttcacg
    31681 ccgtcagcca tcgatttctt gaacgcctta cgtccaagaa tcagccccat tccgccagcg
    31741 cgtttgttga taaccgcagt acgcactgca tcactgaggt cagtttcacc gcctgcagca
    31801 ccaccggagt ttatcaaccc ggcccggccc atatagcagt tagctaactg ataacgcacc
    31861 agatcaatcg ggttttcgct ggttaacttg ctgtacacgc gatcgtcggt ataaccgtaa
    31921 ttaattgctt tatagccgcc gttattttcc gccatttttt gtttgacgat atctgcacct
    31981 atggttgccg ccagatggtt cgcctgacca gtcaggtcgg cggagacatg gtaatcaacg
    32041 ccatctttct taaaggcgga gttacgcaaa taggcccaca gcactgtcac catgcccagc
    32101 tcgtgcgcac gttcaaaagc cgcagaaatt tcttcaatct ggcgacgtga ctcttccgag
    32161 ccaaaataga tagtcgcacc aaccgccacc gcgcccatgt tgaaggcctg ctccacgctg
    32221 gcatacagcg tttgatcgta ggtattcggg taacttagcg tctcgttgtg attaagtttt
    32281 acgagaaatg gaatgcgatg cgcatagcgc cgcgataccg acgccaacac gccgtaagtt
    32341 gatgccacac agttacagcc cgcttcgatc gccagttcaa caatgttttt cgggtcaaag
    32401 tagagcgggt tagcagcaaa tgaagctccg gcagagtgct caacgccctg gtcaaccggc
    32461 agaatagaaa gatatcctgt gccagccaga cgcccggtgt tgtacaacgt ctgcatatta
    32521 cgtaacaccg ctggcgggcg attattgtca atcatcacgc ggtctacgta gtcatgtccg
    32581 gggagataaa gctggtcaga aggaatagtc atacaacggt gctgtaaaag gttgtcggcg
    32641 tctttgccaa gcaactgcgc aatatctgtc attactatgc tcccgtaaat tccgattgga
    32701 tatcggctat ggattgtcct ggcccgcctt ttgcgggcaa tcataatcct ggtcgttacg
    32761 ggcgagtttt tccatctttt ggatgttttt tcagcgtttt ctgttggctc gattcatcag
    32821 aaaaaatgtt agcgcggtca agttttcacc gcaaaggtat ttaaaaggta ttattaagtg
    32881 gtattgtcat cgcgtacctt acattacctg tcatgaagga attaaaagat gaaaacaaca
    32941 acaaagctgt cgttcatgat gtttgttgaa tggtttatct ggggcgcgtg gtttgtgcca
    33001 ttgtggctgt ggttaagtaa aagcggtttt agtgccggag aaattggctg gtcgtatgcc
    33061 tgcacagcca ttgcggcgat cctgtcgcca attctggttg gctccatcac tgaccgcttt
    33121 ttctcggcgc agaaagtgct ggcggtattg atgttcactg gtgcggcgct gatgtatttc
    33181 gctgcgcaac agaccacttt tgccgggttc ttcccgttac tgctggccta ctcgctaacc
    33241 tatatgccga ccattgcgct gactaacagc atcgcttttg ccaacgtgcc ggatgtggag
    33301 cgtgatttcc cgcgcattcg tgtgatgggc actatcggct ggattgcctc tggtctggca
    33361 tgtggtttct tgccgcaaat gctggggtat gccgatatct caccgactaa catcccgctg
    33421 ctgattaccg ccggaagttc tgctctgctc ggtgtgtttg cgtttttcct gcccgacacg
    33481 ccgccaaaaa gcaccggcaa aatggacatt aaagtcatgc tcggcctgga tgcgctgatc
    33541 ctgctgcgcg ataaaaactt cctcgtcttt ttcttctgtt cattcctgtt tgcgatgcca
    33601 ctggcgttct attacatctt tgccaacggt tatctgaccg aagttggcat gaaaaacgcc
    33661 accggctgga tgacgctcgg ccagttctct gaaatattct ttatgctggc attgccgttt
    33721 ttcaccaaac gctttggtat caaaaaggta ttattacttg gtctggtcac cgctgcgatc
    33781 cgctatggct tctttattta cggtagtgcg gatgaatatt tcacctacgc gttactgttc
    33841 ctcggcattt tgctgcacgg cgtaagttac gatttttact acgttaccgc ttacatctat
    33901 gtcgataaaa aagcccccgt gcatatgcgt accgctgcgc agggactgat cacgctctgc
    33961 tgccagggct tcggcagttt gctcggctat cgtcttggcg gtgtgatgat ggaaaagatg
    34021 ttcgcttatc aggaaccggt aaacggactg actttcaact ggtccgggat gtggactttt
    34081 ggcgcggtga tgattgccat tatcgccgtg ctgttcatga tctttttccg cgaatccgac
    34141 aacgaaatta cggctatcaa ggtcgatgat cgcgatattg cgttgacaca aggggaagtt
    34201 aaatgaaaac agaacgtatt ctcggtgctc tttatgggca ggcgttaggg gatgcgatgg
    34261 ggatgccctc cgagctttgg ccacgcagcc gcgttaaagc acactttggc tggattgacc
    34321 gttttctgcc tggaccaaag gagaacaacg cggcctgtta ttttaaccgc gccgaattca
    34381 ccgacgatac ctcgatggcg ctgtgtctgg cggatgcgtt actggaacgt gaaggcaaga
    34441 tcgacccgaa tctgattggg cgtaatattc tcgactgggc gctgcgtttc gacgccttta
    34501 acaaaaacgt actaggtccg acctcgaaga ttgcgcttaa cgccattcgc gacggtaaac
    34561 ccgttgctga actggaaaac aacggcgtga ccaacggcgc agcgatgcgc gtctcgccat
    34621 taggttgttt gcttccggcg cgtgatgttg attcctttat taatgatgtg gcgctggcgt
    34681 cgagcccgac ccataaatcc gatctggcgg ttgcgggcgc ggtagtcatc gcatgggcga
    34741 tttctcgtgc tattgacgga gaaagctggt cagcgattgt tgattcattg ccttcaattg
    34801 cgcgacatgc acagcaaaaa cgcaccacca cgttcagcgc ctcactggca gcgcggctgg
    34861 agattgcgct gaaaattgtg cgcaatgccg acggcactga atccgccagc gaacagcttt
    34921 accaggtcgt tggcgcaggt accagcacta ttgagtccgt tccgtgcgcc attgcgctgg
    34981 ttgaactggc acaaaccgac ccgaatcgct gcgccgtcct gtgcgctaac cttggcggcg
    35041 atacagacac catcggtgct atggcgacgg caatctgcgg cgcgttgcat ggcattaacg
    35101 ctatcgatcc tgcgttaaag gcggagttgg atgcggtaaa tcagcttgat ttcaaccgct
    35161 atgccacagc gctggcgaaa tatcgtcaac aacgggaggc ggcatgagcg gcgctcgatt
    35221 acacacgctg ctgcctgaat taaccacgcg tcagccggtt atggtgattg gcgcggcagt
    35281 cattgatgtg atcgccgacg cttatgccct cccctggcgt gggtgcgata tcgaactgaa
    35341 acagcagagc gttaacgttg gcggctgcgc actgaatatt gccgtggcgt taaagcgcct
    35401 cggcatcgaa gcgggtaacg ccttgccgct cggtcagggc gtgtgggcgg agattattcg
    35461 caaccggatg gcaaaagagg gcttaatcag cctgatcgat aacgccgaag gtgataacgg
    35521 ctggtgtctg gcgctggttg agccggatgg cgaacgcact tttatgtcat tcagcggtgt
    35581 ggaaaatcag tggaatcgcc agtggctggc gcgattaacc gttgcgcctg gcagcctgct
    35641 ctatttttcc ggttatcaac tggcctcgcc ctgcggcgaa ttattagtgg aatggctgga
    35701 aaagctgcaa gacgtgacgc cgtttatcga ttttggccca cgtattggcg atatcccgga
    35761 ggcattactg gcgcggatca tggcctgtcg acctttagtg tcgctcaatc gtcaggaggc
    35821 tgagattgcc gccgaacgtt ttgctttatc cgcagagata acaacacttg gcaagcaatg
    35881 gcaggagaaa tttgccacgc cgttgatcat tcgtctcgat aaagagggcg catggtattt
    35941 cagcaacgac gcttctggct gcattccggc atttcccacg caagttgtag acaccattgg
    36001 ggcgggcgac agtcatgcag gtggcgtact tgccgggttg gcctctggtc tgccactggc
    36061 ggatgccgta ttactgggca atgcagtggc gtcgtgggtt gtcgggcatc ggggcggtga
    36121 ttgtgcgcca acgcgcgagg aactactcct cgcacacaaa aacgtataga tcgctgcgac
    36181 agtggctaat gctgtactca ataggccgtt gctgttggtc aagcgccact tgcttgatca
    36241 ccagcaccgg tattttgcta tctaactgaa tatgtgactg aaactcggca tctggcatcc
    36301 gggcgctaac gcgggaacgt gtacgttgtg ggtaaatgtg ctggctgcgg aaatagtcat
    36361 aaagagaaat tccgatggca tcaacatcat gaattaaatg cgccggaacc cacgattcct
    36421 caatcgatac cgcttcttca tcgacatagc gaatacgctt aagcaaaaag acatcacttc
    36481 ccgcttcgac cgccagctgc tgcgcgactt cttcgggaca tttcacgaca cgcttgttaa
    36541 cccatagcgt atcgggcttt ttcccgcgca ataccacctg ctgagaaaaa cctcgcgctt
    36601 ctttcaacga gtattcgaag atgttgttga tctgcgtgcc gtaaccgcgc gaacgcgtca
    36661 ccacaccctc ttcttccagc gcctgcatcg ccttgcgcac cgtaatgcgt gaaacgccgg
    36721 ttaactgact taaatcacgc tcaccgggca aaatattgcc atgctccagc accccgctgc
    36781 gcacggcatt ttttaccgtt tcggcaaact taatataaag cggcgtgtta tccgccgcta
    36841 aaatacgctc gttcagttgg gcaatcagct gggtatgcgc ttgttccatt tgtctttttc
    36901 ctgccaggga gtctggtggg agtatatcac tgcttcttat ttaaggcgtt tctttaatcc
    36961 catcaacgaa tgcctgcaac tcctcctctg tgccattaaa cacattaaaa tccaccgaac
    37021 cattaatgcc atctacctgc ccacggtcgg aatgctgcca gaaacgccag gccatgccgt
    37081 cattgtccgg gcgacgttga tagtagtgag ccacccacca cggatattca ttgaaatagc
    37141 ccgccagatt ggtgtgataa aaaacggctc ctgagtaaat aaccggcttt tttcccgtac
    37201 ttttttcgac catttttagc cactgactta cccgcttgcg taattctttt gccgataatt
    37261 ttccccgttc ttctacgtcc agcacggcag ggaaatcgcc ttgtgagaaa tccaccgttt
    37321 gcagaaataa tcgcgcctga acaggcgctg ctaccgacgg ggagaaataa tgatacgccc
    37381 cgcgcagcag gccattttcg cggcttagtt gccagttacg cgaaaaatag gggtccacca
    37441 gcttttcgcc ttccgtcgcc ttgataaagg caaattgtaa gcggataccg ttatcgcgca
    37501 tttttgccac ccgctgccag tcgatccgct cctgccagcg cgaaacatct attccgtgaa
    37561 tggtgtaact ggcaggaata tgaatagcga aagatttaac cgggcgataa ccgtagaagt
    37621 ggatgtagtc tgtaatctga actgctgtcg aatagaaaaa attcaccgtc tgacgcggat
    37681 aaattgcaac gatggaaatt agcccaagag cgcataatag agcggtgagt ttttttcgac
    37741 tggtaattct tagctgcatt ggtttcatcc ctgaatgtca gtgccaaagg ctgacaataa
    37801 ccaaagcagc tatagtacgg tgctcaccac caggcgtgga agtggtgaac cggaccaata
    37861 ccgtgaccaa cttccagcgt gtcggcctgg gctaacgccg atgaaagcca gctttttgcc
    37921 tcctgtacgg tgtcagccca gtttgtatgg cgcgggcgta gtgcagccaa cgccgcagag
    37981 agtgtacaac cagtgccgtg ggtgtttttg gtcataatgc gcggtgcggt aaaccgttgt
    38041 tcaccctcgc gggtaaacag ccagtccggg ctttgctcat catccagatg gccacctttc
    38101 attagcactg cgccacagcc catcgccaac agcgctcgcc cttgttccag catttcctgt
    38161 tcggtgcgcg cgtgtggcgc gtcgagcaag gcagcagctt cgggcaagtt tggcgttatt
    38221 aatgaaacct gtggcaataa tcgactgcgt agcgtggcaa ctgccgaagg tgaaagcagt
    38281 gggtcgccac tttttgccag cataacggtg tcgagtacca cgttttggat ctgataacgt
    38341 tgcaaccgtt ctgccaccgc ttcaacaata tcggtttccg ccaacatacc gattttagtg
    38401 gtatcgattc gcacatcgct gaacaccgaa tcgagctggg cggcgacaaa atcaggctca
    38461 atgcgataca ccgactgtac gccacgggta ttttgcgcca ccagtgcagt aataactgag
    38521 caaccataag cgccaagtgc cgagaaggtt ttaagatcgg cctgaatccc cgcaccacca
    38581 ctcggatcag taccggcaat cgtcagagcg ttaattcgtt tcatgcctgc acctcctgcg
    38641 tcaattgcca gagcgcatca aggaaatgtg gaacaaaact gcctggcccc tcgcttctgg
    38701 cgactgcgcg ttctccggct tgtttcatcc agtgacaggc agatgcgaca ttttccagca
    38761 tatcgcctgg taacgcacaa caggcagcga caaccgccga taatgcacag ccagttccta
    38821 ccactttggt cattaacgga tcgccaccgt gaatgccaac ggcgcgatgg ccatcggtaa
    38881 cataatccac ctcgccagtg accacgacga ttgcgccagt ttcccgtgcc agtgtttgtg
    38941 cagcgggtat cgcgttaact gcggcgtcag tggtatccac tccccgtcca ccattagcaa
    39001 cgccagctaa tgccatgatt tccgaagcat taccacgtat cgctgccggt ttaaaagata
    39061 aaagttcatg acaaaaatgg cggcgataat cgagcgcacc aaccgctact ggatcaagcg
    39121 tccagggtgt ttgagagctt tttgcttgct caacggcagc acgcatcgcc tgtgcgcgtg
    39181 gctgcgtcag tgtgccaacg ttaatcaaca aggcactggc gatagccgca aactgactgg
    39241 cctcttcggt ttcgataacc atcgctggcg atgcaccgag cgccagcaag gtattggcag
    39301 taaaggtttg caccacatcg ttggtcatgc agtgcacaag aggggaatgt tggtgaaaaa
    39361 ggtgtaacgt gtgcgcagat tgcgcggaac tcagcaggtc gacttgcata gtttgctcct
    39421 gccataacgt gaagaagcaa tgacctggtg gtccgtgact tccctacgct ggcattatcc
    39481 agatcaggtg atacgggtat ttctcagcct tcacgcagaa gggcaccccg agtcgtttgg
    39541 ttgaatctct atattttgtg ccagcttagg ggaagaggct tgcagatgca agctcgcctc
    39601 cattcattac gtcttcactg tgtaccccat atcagtaatg aaaaagaaac aattcacacc
    39661 catcattcag gtggtaaaaa ctgtagacta tcttaattag ctaatttata ttatttatct
    39721 aaatttgata ggtgagataa aaatgacaga aacccagatc tacgagaaca ttaagcaagc
    39781 cattagcagc gcaccacgaa atagccaaac gatggaaatg cacctccaga tgatcaaata
    39841 cgctgaccat ctgaaaaacg tcacggcaaa agagttttgc gaaggtgtag gacttaaagc
    39901 cagctttgct acagagttca gcaaaatgag aaatcttacc gagcgactca aagccgccgg
    39961 acttgatacc acgaagttat aacaaacgaa atcatcaata tattactttc gttttccagg
    40021 gttccctgta tatgtaccta tgatcataaa aaccagttac caccacgtag ctgataccat
    40081 aatattttca tttttacttt aaataaatag ataaatttag agagtggaat aattaaggtc
    40141 tattttctct agctttttga gattgatagt tgagaatgat aattctttca tctcaataac
    40201 accgaagttt gatagctgtt taagccacag ttgaagacca gcatcagagc aacctgctcg
    40261 atatgttaca tcactacatt ttattagtgt tgatttcctc ttatagagat aaataaggac
    40321 ttcatagatg cgttttccgg tgtcattgga tacgcgctat acccccattc ttggctaaat
    40381 cttggttcat aatcatactt taattaaaat ggatct
//
LOCUS       DAZFBR010000030        40129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFBR010000030 DAZFBR010000000
VERSION     DAZFBR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.030"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(40..1899)
                     /gene="gss"
                     /locus_tag="ACHJZ2_001824"
     CDS             complement(40..1899)
                     /gene="gss"
                     /locus_tag="ACHJZ2_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO4567949.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIVTQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2104..2970
                     /gene="yghU"
                     /locus_tag="ACHJZ2_001825"
     CDS             2104..2970
                     /gene="yghU"
                     /locus_tag="ACHJZ2_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO4567950.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3093..3341)
                     /gene="hybG"
                     /locus_tag="ACHJZ2_001826"
     CDS             complement(3093..3341)
                     /gene="hybG"
                     /locus_tag="ACHJZ2_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO4567951.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3354..3695)
                     /gene="hypA"
                     /locus_tag="ACHJZ2_001827"
     CDS             complement(3354..3695)
                     /gene="hypA"
                     /locus_tag="ACHJZ2_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4567952.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3688..4176)
                     /gene="hybE"
                     /locus_tag="ACHJZ2_001828"
     CDS             complement(3688..4176)
                     /gene="hybE"
                     /locus_tag="ACHJZ2_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO4567953.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4169..4663)
                     /gene="hybD"
                     /locus_tag="ACHJZ2_001829"
     CDS             complement(4169..4663)
                     /gene="hybD"
                     /locus_tag="ACHJZ2_001829"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO4567954.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4663..6366)
                     /gene="hybC"
                     /locus_tag="ACHJZ2_001830"
     CDS             complement(4663..6366)
                     /gene="hybC"
                     /locus_tag="ACHJZ2_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO4567955.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6363..7541)
                     /gene="hybB"
                     /locus_tag="ACHJZ2_001831"
     CDS             complement(6363..7541)
                     /gene="hybB"
                     /locus_tag="ACHJZ2_001831"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO4567956.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7531..8517)
                     /gene="hybA"
                     /locus_tag="ACHJZ2_001832"
     CDS             complement(7531..8517)
                     /gene="hybA"
                     /locus_tag="ACHJZ2_001832"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO4567957.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8520..9638)
                     /gene="hybO"
                     /locus_tag="ACHJZ2_001833"
     CDS             complement(8520..9638)
                     /gene="hybO"
                     /locus_tag="ACHJZ2_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO4567958.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9827..10114)
                     /gene="yghW"
                     /locus_tag="ACHJZ2_001834"
     CDS             complement(9827..10114)
                     /gene="yghW"
                     /locus_tag="ACHJZ2_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO4567959.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10233..11120)
                     /gene="yghX"
                     /locus_tag="ACHJZ2_001835"
     CDS             complement(10233..11120)
                     /gene="yghX"
                     /locus_tag="ACHJZ2_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO4567960.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11277..12317
                     /gene="gpr"
                     /locus_tag="ACHJZ2_001836"
     CDS             11277..12317
                     /gene="gpr"
                     /locus_tag="ACHJZ2_001836"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO4567961.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12357..12851)
                     /gene="yqhA"
                     /locus_tag="ACHJZ2_001837"
     CDS             complement(12357..12851)
                     /gene="yqhA"
                     /locus_tag="ACHJZ2_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO4567962.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13042..13926
                     /gene="yghA"
                     /locus_tag="ACHJZ2_001838"
     CDS             13042..13926
                     /gene="yghA"
                     /locus_tag="ACHJZ2_001838"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO4567963.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14198..14623)
                     /gene="exbD"
                     /locus_tag="ACHJZ2_001839"
     CDS             complement(14198..14623)
                     /gene="exbD"
                     /locus_tag="ACHJZ2_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO4567964.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14630..15364)
                     /gene="exbB"
                     /locus_tag="ACHJZ2_001840"
     CDS             complement(14630..15364)
                     /gene="exbB"
                     /locus_tag="ACHJZ2_001840"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO4567965.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15616..16803
                     /gene="metC"
                     /locus_tag="ACHJZ2_001841"
     CDS             15616..16803
                     /gene="metC"
                     /locus_tag="ACHJZ2_001841"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO4567966.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16943..17602
                     /gene="yghB"
                     /locus_tag="ACHJZ2_001842"
     CDS             16943..17602
                     /gene="yghB"
                     /locus_tag="ACHJZ2_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO4567967.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17642..18541)
                     /gene="yqhC"
                     /locus_tag="ACHJZ2_001843"
     CDS             complement(17642..18541)
                     /gene="yqhC"
                     /locus_tag="ACHJZ2_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO4567968.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18735..19898
                     /gene="yqhD"
                     /locus_tag="ACHJZ2_001844"
     CDS             18735..19898
                     /gene="yqhD"
                     /locus_tag="ACHJZ2_001844"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO4567969.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20003..20830
                     /gene="dkgA"
                     /locus_tag="ACHJZ2_001845"
     CDS             20003..20830
                     /gene="dkgA"
                     /locus_tag="ACHJZ2_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO4567970.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21030..21952
                     /gene="yqhG"
                     /locus_tag="ACHJZ2_001846"
                     /pseudo
     CDS             21030..21952
                     /gene="yqhG"
                     /locus_tag="ACHJZ2_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            22001..22258
                     /gene="yqhH"
                     /locus_tag="ACHJZ2_001847"
     CDS             22001..22258
                     /gene="yqhH"
                     /locus_tag="ACHJZ2_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO4567971.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22301..24520)
                     /gene="ygiQ"
                     /locus_tag="ACHJZ2_001848"
     CDS             complement(22301..24520)
                     /gene="ygiQ"
                     /locus_tag="ACHJZ2_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO4567972.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24631..26043)
                     /gene="ftsP"
                     /locus_tag="ACHJZ2_001849"
     CDS             complement(24631..26043)
                     /gene="ftsP"
                     /locus_tag="ACHJZ2_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO4567973.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26118..26855)
                     /gene="plsC"
                     /locus_tag="ACHJZ2_001850"
                     /gene_synonym="parF"
     CDS             complement(26118..26855)
                     /gene="plsC"
                     /locus_tag="ACHJZ2_001850"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO4567974.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27089..29347)
                     /gene="parC"
                     /locus_tag="ACHJZ2_001851"
     CDS             complement(27089..29347)
                     /gene="parC"
                     /locus_tag="ACHJZ2_001851"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO4567975.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29485..31092)
                     /gene="ygiS"
                     /locus_tag="ACHJZ2_001852"
     CDS             complement(29485..31092)
                     /gene="ygiS"
                     /locus_tag="ACHJZ2_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4567976.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31201..31683)
                     /gene="ygiV"
                     /locus_tag="ACHJZ2_001853"
     CDS             complement(31201..31683)
                     /gene="ygiV"
                     /locus_tag="ACHJZ2_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO4567977.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(31736..32128)
                     /gene="ygiW"
                     /locus_tag="ACHJZ2_001854"
     CDS             complement(31736..32128)
                     /gene="ygiW"
                     /locus_tag="ACHJZ2_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO4567978.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32280..32939
                     /gene="qseB"
                     /locus_tag="ACHJZ2_001855"
     CDS             32280..32939
                     /gene="qseB"
                     /locus_tag="ACHJZ2_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO4567979.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32936..34285
                     /gene="qseC"
                     /locus_tag="ACHJZ2_001856"
     CDS             32936..34285
                     /gene="qseC"
                     /locus_tag="ACHJZ2_001856"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO4567980.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            34395..34976
                     /gene="mdaB"
                     /locus_tag="ACHJZ2_001857"
     CDS             34395..34976
                     /gene="mdaB"
                     /locus_tag="ACHJZ2_001857"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO4567981.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35007..35321
                     /gene="ygiN"
                     /locus_tag="ACHJZ2_001858"
     CDS             35007..35321
                     /gene="ygiN"
                     /locus_tag="ACHJZ2_001858"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO4567982.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(35366..36253)
                     /locus_tag="ACHJZ2_001859"
     CDS             complement(35366..36253)
                     /locus_tag="ACHJZ2_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO4567983.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(36250..37197)
                     /locus_tag="ACHJZ2_001860"
     CDS             complement(36250..37197)
                     /locus_tag="ACHJZ2_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4567984.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(37208..38245)
                     /locus_tag="ACHJZ2_001861"
     CDS             complement(37208..38245)
                     /locus_tag="ACHJZ2_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO4567985.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(38254..39237)
                     /locus_tag="ACHJZ2_001862"
     CDS             complement(38254..39237)
                     /locus_tag="ACHJZ2_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO4567986.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(39234..40043)
                     /locus_tag="ACHJZ2_001863"
     CDS             complement(39234..40043)
                     /locus_tag="ACHJZ2_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4567987.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
BASE COUNT        10000 a        10791 c        10065 g         9273 t
ORIGIN      
        1 ctattcttca taatcagtac gggattgcta caacatgtat tactctttca ccacaattaa
       61 cggttcaata tcactctctt ttttaataac cagtgattca tcaccgcgca aacacgtccc
      121 accgtagttg ccgccaacgg tgaaggtaca tacctgaatg tatttaccgt ccactttcgg
      181 caaacaccac agttgctgat agatgttttt ctgctcggca aatttaccac tggttttgtc
      241 cagcacctct tcatgatggc tgacgaggtc gatgttgctg ccacagcgac cggcgatcgg
      301 tttcactgcg taacctgttt tcaccaattc atcattaaca gtgaaatcgg tatccagcag
      361 gtaacgatgg tgcgggaaca gcgaccagag gatcggcaga atcgctttgt tgccggggat
      421 caccgtccac agcggctcaa agaccagcac ttccgggcgc agcaatacgt cgataagacg
      481 cacttcattt tgcggatgac cggtacggat cggcaccgca gcaaactcac ggtcgctaac
      541 ttcacgaatc tgatcaaacg cggtttccca cgcccaggtt ttccacacgc agttaaccag
      601 tcgcccttcc ccatcaatca gttgcccggc agcatcccag cccagttcat ccagcccgcg
      661 caagatacgc gtttcaaagc ccgcctggtg cagcgcctgc tccataaact gcgcgtgata
      721 gttttcctcg atatctttgt cctgcatgat atggacaaac ggacgtgcac gactgtgttt
      781 ccaggcacca gccagttcgt taatcagccc ttccgccgga ttgaagccgt tgcctttata
      841 gccctgctcc gcccaacgtt cgaggatcaa gcccgcttcg gtatgacagg aggcggagtc
      901 ggcgttgtac tcgtaaacct tcagaccacg ctcatccatg cagaaatcca tacgaccggt
      961 gatcatatgg tgacggcgac gctgccagga gagacgcaaa cgtggccaga ggattttcgg
     1021 aatgtcgaac agcgccagca ggttgtcatc tttcagcacc ttgtcggttg cgtgaagata
     1081 catcaggtgc agctcgttgg tggctttaat cagctcctgc tcggcgctct cggtaatggt
     1141 gtagtaatga tacggatcct gattgatcac ctgaccgttg gcctgcacat aggcgttttg
     1201 cagcggatct ttttcatcca gccatttacc gtcaaactgg cctttgtttt ccagacgcgc
     1261 tccgctgatt ttcagcagct cgcctgcaat ttccggctgc ggtaagctgt attcagtatc
     1321 ttccgtctgg atcatccagc ccagaatggt ggtgtcatca aaagtgtctt taagggtata
     1381 gcagccgttt tcgaccacca tctccagttc gcgcgtccac tgttgccctt gcggcaacgg
     1441 ggaatgaatc acgttctgtt ccgcaatacg gactttgttg ccatgtaatt gggtaacgat
     1501 ggcgacatgg ccagtgtctt taaattcacc gcctttatcc cagataagaa gcgcacccgc
     1561 gaccggcgca cgcggcgagc cgttaggaaa tgcctgcaat ggcaggatgt tgtcattaac
     1621 cacttcacgc aggaagcgca gcgagaaaat ctcccacgcc atacccacgt cagtaaagac
     1681 cacaccgtag ttcagaaaga gaaaacggcg agcaaattca acgcattgcc atttgtggcc
     1741 catatattcg tcgtcgatat agctacggaa tacggcgtca tcttcgtatt cctgcggatc
     1801 gagagaactg taatctgaag agtagattgc taccccacct ggggcgtagc ccaataatgt
     1861 cccgaacggg gcatcctggc tggtcgttcc tttgctcatc actttacctt taacaataca
     1921 acctaagcag ttggcgtaat tgttgtgctc tgattacctg ctcagcgaca ttaaccggac
     1981 agaggtcaga ctaatcatac acctcatcgc agcggctggc gcaaagggtt aaaaaattca
     2041 cattctgtac agcaagtgac ctgctacact gcttcaacac taccactcag aaggcaactc
     2101 actatgacag acaatactta tcagcccgcg aaagtctgga cgtgggataa atccgctggc
     2161 ggcgcgttcg ccaatatcaa tcgcccggtt tctggtccga cgcatgaaaa aacgctgccg
     2221 gtgggcaaac atccattaca actttattcg ctgggaacgc cgaacggtca gaaagtaacg
     2281 attatgcttg aggagctgct cgcgctgggc gttactggcg cagagtacga tgcctggctg
     2341 atacgcatag gcgatggtga tcaattctcc agcggctttg tcgaagtgaa cccaaattcg
     2401 aagatcccgg cgctgcgcga tcatacccat aatccgccga tccgcgtgtt tgagtcagga
     2461 tcgattctgc tttatctggc ggagaaattt ggctacttcc tgccgcagga tctggcaaaa
     2521 cggactgaaa cgatgaactg gctgttctgg ttacagggcg cggcaccgtt cctcggcggt
     2581 ggttttggtc acttttacca ttacgcgccg gtgaaaattg agtacgccat caatcgcttt
     2641 accatggaag ccaagcgcct gctcgacgtg ctggataagc aactggcgca gcataagttt
     2701 gttgcgggcg atgagtacac cattgcggat atggcgattt ggccgtggtt tggcaacgtg
     2761 gtgctcggcg gcgtgtatga tgccgcagag tttctggatg cgggaagtta taaacatgtg
     2821 caacgctggg cgaaagaagt gggcgaacgt ccggcagtga agcgtgggcg tattgttaac
     2881 cgcaccaacg ggccgctgaa cgagcagttg catgagcgcc atgacgccag tgattttgag
     2941 acgaatacgg aagataagcg tcaggggtaa ggattggtgt tcgtcgcagc aagccatcca
     3001 ggccggataa ggcgttcacg ccgcatccgg caatcgagca caatgcctga tgcgacgctg
     3061 ccgcgtctta tcagacctac gaaagctaat catcacgcgc tggtaatgtc gtaatccatt
     3121 tggcgcagtg cgtctaatgt ggctttggct tcatcttcgt cgatgatgct catggcaaat
     3181 ccgacgtgta ccagcaccca ctggcccagc agatcggcag ggttaccttc acaaatcagg
     3241 gcgatattca cgtcgcgctt gataccacat acttcaacct gcgcaagctg gtgaatatct
     3301 tcaccgacag ccagcacctg gcctggaacg ccaatacaca ttgttaactc cggttattca
     3361 acttcaatac ttttgacgat cagcgaatcg ccggtatcga cacgcaaccg ctcgccgtga
     3421 cagagcggac actgcgcatc gtgctgatga acctccacca cctggctgca atcccagcac
     3481 caggcctggg cgggtttata gacgatatgt aaatcgcacc cttgcgccac cgttccgtgg
     3541 cagacaattt caaaactaaa acggacggcg ctctcctcaa cgcaggagag cgcgccaatt
     3601 tccagccaca cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg ctggataatt
     3661 tcaacggcgc tctggcaaag cgacaactca tgcattttcg ccactcctgc gaccaaacag
     3721 cagggcgcga cgccctgcgt gtggtacatc cggattcgtg actggcagcg aaaggatcat
     3781 tcgtgcgcag tcatccgtca ggcgttggcc ctcttcaatc gacatactgt gcgaaagcgg
     3841 tgacatcagc gaacaggaga ggtattgcga aacaccgtcc agttcgccaa cggtaaaggt
     3901 catggtgcca tacggcagtt gcagaccaat tttttcactc actttgcgca gcggccagag
     3961 ttgatccggg ccggggaaga taactgcact cagcatccac ggggtgatca cacaccccgt
     4021 ccactgacct tcgaacagcg taaaatcaga aacatacacc ggcattgaag gatgcagaaa
     4081 agagagagcg tgcatcgaac gccgggcaat ttcttcaaac gctgcctgta cttgcgcctt
     4141 cggggaggtc tggaaacctg ctatctcttc agtcatgaat cgcctcccgt gggatggctt
     4201 ccacgccaga ttcacgcagc gcagccagaa cctgctcaag cgcaggttca atcattgctt
     4261 caaccgtcgg cgttaagccg atgtgtggct ccagcgattc cggaattacg ccgaccaggg
     4321 tcagcttttt cggaaactcg ccggtgaagc gcagggccga caagacgtcg gccaggccaa
     4381 gctgatgcgg agagattttg ttggtaaaca acgccggaac ttcttcatcc cgcaggatca
     4441 tcatcgttcc cggcgcgctc ttcttcgaca caatggcatc cgcaataatc agatgatcgc
     4501 gatttgccat gtcgccaagc agctccattc ccgccgtgcc gccatcgagg atctcaacat
     4561 aatccggcag aatgtatcgt tgctctaacg cttcgacaat ccgcacaccg atggcttcat
     4621 cggtcagcaa aatattgccg acccctaaga ctaaaatacg cattacagaa ccttcactga
     4681 aaccacttcg ttgccgtcag catccactac gtgtaccgca caggccatgc acgggtcgaa
     4741 ggagtgaatg gtacgcacca cttccagcgg tttattcgga tcagcaaccg gtgtacccac
     4801 cagcgactgc tcgtaaggac cgacgtcatc attgaagtta cgcgggccag agttccaggt
     4861 tgatggaaca actgcctggt agttgctgat gataccgtct ttgatcacca tccagtgaga
     4921 gagcataccg cgcggcgctt cgaggaagcc aacacctttg aactcacccg ttgccggaat
     4981 gttcggcttc acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca gtgcactgta
     5041 ttggttttgc aggatatcct gcaattcaca gcagtgaacg gtacgaccaa taatacggcc
     5101 cagcgtggag tgcagctgtg ccacttccag cgtgttgcca gtcagtttct gataaatcgc
     5161 aacgatttca ttcagtttgt tctgggtaga ttcgcgacct gccgccagtt tcaccagcat
     5221 attagccagc ggacccactt ctaccgtttt gccgtagaaa gtcggtgatt tcacccagga
     5281 atatttcccg tcgtcagacc aaccatcata agccggaatg gtggtacctt cccacggtgc
     5341 ctgcggcgct tcgtctttat accaggagtg cttcgcgctt tcctgaatgc ctttgatcag
     5401 gtattcatcg gaatgagaag tgatcggacg atacgaggac agatccgcat tctcaatgta
     5461 gccgcccggg aacaggaagc tgccgttttt actgtcggtc gggaattccg gcacgctcag
     5521 gtagttcacc gcacctttac cgcgcgtcag ccattccggg tagaacgcag cgataaccgc
     5581 agtatcgacc ttgtaaacct gctcaacaaa gtcactcagc ttgtcgatga aagacttgat
     5641 gtacatcagg cgctcaaggt tcagcacgcc cagaccgtcg aggttgattg ggttagcgac
     5701 cccacctacc gccaggttct gaatgtgcgg cgttttaccg cccagcagcg ccacgacgcg
     5761 gttagcgtca cgctggcact ccagcgcctg caggtagtgc gctaccgcaa tcaggttcac
     5821 ttccggcggc agtttcatcg ccgggtgacc ccagtagcca ttggcgaaaa tacccaactg
     5881 accgctggca accagatctt tgatcttgtt ctgaactttg gtgaactctt ccggactgtt
     5941 caggtgccag gtcgaaacac ctttcagcat ttcggaggct ttggttgggt cagcttgcag
     6001 tgcagaagtg atgtccaccc agtccagcgc cgaaagctga tagaaatgaa cgatatggtc
     6061 atgcgtggtg tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa ccggaacgtc
     6121 gatattcagc gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg tagtacatac
     6181 gccacagata cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt tcacgatctc
     6241 ttccatgccg cgccacatgg taccggaagc ccatgctttc gaaacgacgc cattttcgat
     6301 ttcgcaatcg atgcgtaaat gcccctcaat acgggttacc ggatcaatag taattctctg
     6361 gctcatgctt tgctcgcctc atgacgatta tgatcgtttt gttttaaagg aggaagtatc
     6421 ggcagtagac gaatgagtac gatgtaagcg caaatctcaa tagccacaaa accaatagaa
     6481 atcaacagtt cttcccaggt cgggaagtag gcgtaaccgc cgcccgggtt gaatgccacc
     6541 agcgaatagg tcagacgcca ggttgcacaa cctaacagcg cgctcagtgc tgacaggaac
     6601 agcatgcggg aatcattacg cagcttcgcc acacgcagaa cgaccagcgg gaagagcatc
     6661 agcaggactt caatccagaa catcacggag tagaagtcac cggcaaacgc taacgacagc
     6721 ttgtcgcgat agataagctc gccaaagcgc agcacgatga aaatcgccag caacacactg
     6781 atggtgttgg tcagcttgac gaataggctc ttttcatccg gaccgttgcc acgcagaccc
     6841 gcctgcacca gtgaaccttc aaagatgaca atcgagaagc ccatgatgaa cgccgtcagc
     6901 agcgagaaca gcggcaacat ttcatagctc tgccacagcg gatgcacctt gtagcccgcc
     6961 gagatcatca gcgaccccat tgaagactgg tgcatggtcg gcagcagcgc accgagcgcg
     7021 atgatgaaga acatcacctt gttcagacgc tgtagcgaca ccttccagcc cagacgttca
     7081 aacagtgccg gagcaaactc cagagccatc acgccgatgt agatggtcat acagaccgcc
     7141 gtctcgaaca gtaccgagtt cacgttgaag tgacccggaa tgtagaagta cggcaggttc
     7201 cagtagcgac ccacgtcgat agtgatcgac aagccaccca gtgagtaacc aaacagactc
     7261 gccaacagcg ccggacgcac cagcggatgg tattgcccac ggttaaagac gtataccgcc
     7321 cacgcaagcg cccagccgcc acaggcaaag ccggtgccaa tcagcaggtc aaacgcgatc
     7381 cacacgcccc acgggaagcc gccgttcagg tcagagacag agcccagacc gaacaccaga
     7441 cgcttcacaa tcaggagcat acagatgacg attaacggtc caaaaatcat gaccggttta
     7501 ctgatgattt tgccgcccag cggttgtgga tcatgactca tgatcgtctc ctccgtcgtg
     7561 atggtcgttt ttggtgttgc gacgaaccag cacggtcaag cccgccagca cagccagtgg
     7621 tagcatcatg cctttataca gggtgtgttg aatattttcg gaacgcgcac cggtagaaag
     7681 atcgtccagt ttcggcaggt cgagattttc ataaggcaca cccgtcagta ccagaacctg
     7741 agtaccgccg ccctctttct cgccgtacag atgcggataa tatttcggca ccgtatgcag
     7801 gtaagtgtcg ccagatttca gcgtctgacg cggatagtgg tattcgctgc caggcttcag
     7861 cgccagacgt tttttcgcct ccgccatcag ctcttcacgc gtaccaaaaa tcaccgcgcc
     7921 cgccgggcac acttctacgc agccaggcag accgccttta tcgagacgtt ccacaccttt
     7981 ctggttgcac agctcgcact tatgcagcgc accaaacggg ttgttgtagt cgtacttcgg
     8041 cacgttgtac ggacaggcga ccatgcagta acggcaaccg gtgcacacgt ctttgtcgta
     8101 atggacaatg ccggttttcg gatctttttt cagtgcagag accgggcaca cagagacaca
     8161 gttcggatcg acgcagtgca tacactgttt cttaatgtac gcgtagccgt tctcctcctg
     8221 gtctttgttg acccctgtgc cgctggtcca cacttgaatg atgttattgg tatacggcga
     8281 cagtttgtcg ttgttcgacc aggtctgttc cccttgcggg ttacgttcag ggaagttgat
     8341 atcctgacac ttggtgacgc aagcctggca gcctacgcac aaggtcgagt cgtacaacat
     8401 ccccagcgat cccggaattg gcgggcggtt ttcagcagcc gcatgactga cagacggcag
     8461 cgcgcccgtc agcaatgccc cgcaggaggc tgctttaata aaattacgtc tgttcacggt
     8521 tattctcccc gtgagtcagc gttatctttc ttttgctgac gacccagttc acgcaccgcc
     8581 atcacgctga caccggcaac cagcccaacc acaccgccga gcaaaccaat agcgcctgca
     8641 gagacgttgc cgccctcttt agcgttaaca tccggtttct gtgaacgcgg agtctgattt
     8701 tcgacgttgg caagctgatg gatgccttta tggaagccga taccttcttc gttacagcca
     8761 tagcacgggt gaccaatcgc caccggccac acaccgccaa catcgcagaa ttgcagcgtt
     8821 gagcagttgc cgtaagtttc tggcccttta cagccgaggt ggtacaggca ccagccttcg
     8881 cggtggcctt catcaccgaa ctctttggca aaacgaccag catcgaagtg cgggcgacgt
     8941 tcgcagtgtt cgtgaatcag acggccatag gcgaaggtcg gacggttttt gtcatccagt
     9001 ttcggcggtt tgccgtaagt gatgatgtgc gcaacggtcg cgaggaagtt gtgcgggttc
     9061 ggcgggcagc ccggaatatt gataacggtt ttgcctggca gaacttcttg caagctgact
     9121 gcgccagttg ggttaactcc agctgcggca acaccgcccc acgcagagca ggaaccgata
     9181 gcgataatgg ctgctgcgcc ttccgccgct ttgcggatgt gatccacaat cggctcaccg
     9241 gcaaccatgc aataaatacc gttatctttt aatgggatgg aaccatccac cactaacaca
     9301 tactgccctt tgtacttctc gagagcgtta tgtttgttct cttcgacctg atgaccgaag
     9361 gcggcggaaa gcacttcgtg atactccaga gagatagtct ccagtacgag gttttctacc
     9421 gttggatgcg ttgcacgaag cagagattcc gtacaaccgg tgcactcctg cgcgccaatc
     9481 cagataactg gcggacgctg cgggttagta accgattcgg ccatctctgc agcggctttg
     9541 ctacttaacc ccatggtggc ggctaatgct gcacaaagct tcatgaaatc acgacggtta
     9601 atgccgtgag aatggatgag ggtgttatct ccagtcattt atagttattc cgttgcgaag
     9661 acctggcata tattttgcct caatcgcaaa atcaataatg cgatcgatgc gccatttacc
     9721 acacatttat tatggttatc gatatcatga tactgcggcg gggcgctaaa acgaagggaa
     9781 gaagcattag tgtaattaat taaagcaaga taatacgtat gtttgatcaa ttttcgaggc
     9841 gataaccggc cataaaaaag cgcaacgtag aacaggaatt attctcacga aagaaatcca
     9901 ttaccatctc tttatccagt ccatagcggc gcgtcagaat acccgcttcc caggcgctaa
     9961 gctggcgatc tccggtcttt tcgtacatcc ggtgtgagta gcctaataca aaaccgcgtt
    10021 tataatcggc acagaatttt gtcacattaa ccgcactgtc ggcatgcgtt gcttttaatc
    10081 ccgccattaa gcctttacca aaatggttat tcatcgttca cctcattcga tatatatcgt
    10141 aatatatagc gatattttag gcaaagaacg acttttgcgc acgctatttt ttcatgcttg
    10201 tttaagacgt aatcccgtaa gagataaacc tatcaggaga gatatttatc gaaccatttc
    10261 agtgtccttt gccaggcaag atcggcggca gatttgtcat aacggggcgt ggaatcatta
    10321 tggaatccgt gattaacccc cggatagata tacgcctcat aaaccttatt attggctttc
    10381 aacgccgcct cgtaagcagg ccagccctcg ttgattcggg tgtccagttc cgcgaagtgg
    10441 agtagtaaag gcgcttcaat cttcgccaca tcggcagtgg gtgcctgacg accataaaac
    10501 ggcactgcgc aggccagttc cgggtaagcg accgctgccg cgttcgatac gccaccgcca
    10561 tagcaaaatc cggtaatacc cactttgcct gtcgcttgcg gatagcgttg cataaactca
    10621 attgcggcaa agaaatcatt catcagtttg gttggatcaa cctgctgttg cagctcacga
    10681 cctttatcat catttcccgg ataacctcca acggaattta agccgtcagg tgccagggcg
    10741 atataccccg ccttcgccac tcgccgtgcc acatcttcga tatacggatt cagtccacga
    10801 ttctcatgca ccaccaccac ggctggcgtt ttgccgctca tttttgcggg tttaaccaga
    10861 taaccccgta cctcgccgtg accatttggc gaaggatacg tgatgtactc agcaaaaatc
    10921 tccgggtcgg taaactctac ctgagtcgcc agcgcataat ttggcttgag caaatcaaac
    10981 aacgctaatg ccgtcatccc gcctaccgca tacttcgccg caaggttgag gaactcacgt
    11041 ttcgagattt tcccgtgagc gtaatagtcg tagtaatcca gcaactcttg tgggaaatct
    11101 ttggcggtta aacgcggcat cgttgcactc ctcagttggt gtttttttaa gcaaagcata
    11161 agcacgtgtt tttgcccagt ttttcgtcac tctgtgagcc agactacggg atacgcgctg
    11221 gcgaatcgct aaactagaaa cattgtttcg aaattgaacg gtggaaagga gaggtcatgg
    11281 tctggttagc gaatcccgaa cgttacgggc agatgcaata ccgctattgc ggaaaaagcg
    11341 gtttacgcct gcccgcgtta tcgctcggtt tatggcacaa tttcggtcac gttaacgcgc
    11401 tggaatcaca gcgtgcgatc ctgcgtaaag cgtttgattt aggcattacg cactttgatt
    11461 tagccaacaa ttacgggccg cctccaggaa gcgcagaaga gaactttggt cgcctgctgc
    11521 gggaggattt tgccgcttat cgcgatgaac tgattatctc taccaaagct ggctacgata
    11581 tgtggcccgg cccttacggc tctggcggtt cacgtaaata cctgctcgcc agcctcgacc
    11641 aaagcctgaa gcgtatgggg cttgagtatg tcgatatctt ttactctcat cgcgtcgatg
    11701 aaaatacgcc gatggaagaa accgcctctg cgctggctca tgcggtacaa agcggtaagg
    11761 cgctgtatgt cgggatctcc tcttactcac cagagcggac gcaaaaaatg gtcgagttgc
    11821 tgcacgagtg gaaaattccg ctgttaattc atcaaccttc gtacaattta ctaaaccgct
    11881 gggtggataa aagcggcctg ctggataccc tgcaaaataa cggcgtgggc tgcattgcct
    11941 ttactcctct ggctcaggga ttgctgaccg gaaaatatct caacggcatt cctgaagatt
    12001 cacggatgca tcgtgaaggg aataaagttc gtggtctgac gccgaaaatg ctcaccgaag
    12061 ccaacctcaa cagcctacgc ttattgaatg aaatggcaca gcagcgtgga caatcaatgg
    12121 cgcaaatggc gttaagctgg ttgctgaaag atgagcgagt gacgtcggta ttggttggtg
    12181 ccagccgcgc ggagcaactt gaggagaacg tgcaggcgct gaataatctg acatttagca
    12241 ccgaggagct ggcgcagatc gatcagcata tcgccgatgg cgagctgaat ctgtggcagg
    12301 cgtcttccga taaatgacct gttaataacg ggcgcgagaa ccgcgcccga atcagatcag
    12361 tgattatgac gagtcagtcg gtcaagatag cccattacaa atgcagagag cacaaacgtc
    12421 agatggataa tgacgtacca catcagtttg ttatcaggga catttttcgc atccataaag
    12481 acgcgcagta agtgaatgga agaaattgcc acaatcgacg ctgctacttt gtttttcagc
    12541 gacgttgcat ccattttccc cagccagttc agcttctctt tgttctcgga gatatcgagt
    12601 tgtgagacga aattctcata accggaaaac atcaccatca ccagcaaacc gccaaccagc
    12661 gtcatatcca ccagcgacag caacacgagg atcaaatctg attccgccat cgagaagata
    12721 ttcggcagta cgtgaataat ctcctggaag aacttcagcg ccagggcaac taacgccagc
    12781 gaaaggccaa agtacacggg ggcaagcagc cagcgagaag catacattgc attttcaaga
    12841 aaacgttcca tacattcctg tcgtgtagtt aaaacgggca acagtatatc gcaattgagt
    12901 aaacagcgcg caacagcaaa tcaacatatc cgttaaatct ttgtcgcata attctgaggg
    12961 ttagggatct tcctggttcc cttatcaacg catcgagagc gtctaccctt aaaggacgtt
    13021 gaacctgaag ggagaaaaac gatgtctcat ttaaaagacc cgaccacgca gtattacact
    13081 ggtgaatatc ccaaacagaa acaaccgacg ccaggcatcc aggcgaagat gacaccggta
    13141 ccggattgcg gcgagaaaac ctatgttggt agcggtcgcc tgaaagatcg taaagccctg
    13201 gtgacagggg gcgattccgg aatcggtcgc gctgccgcca tcgcttacgc gcgtgaaggg
    13261 gctgacgtgg cgatcagtta tcttcccgtg gaagaagaag acgctcagga tgtgaaaaag
    13321 atcattgaag aatgcggacg caaagccgtt ctgctgccag gcgatttaag cgatgagaaa
    13381 tttgcccgtt cgctggttca cgaagcgcat aaggccttag gtgggctgga tattatggcg
    13441 ctggtcgccg ggaaacaggt tgccattcct gatattgcag acctcaccag cgaacagttt
    13501 caaaagacct ttgccattaa cgttttcgcg ctgttctggc taacccagga agcgatcccc
    13561 ctgctaccga aaggtgcaag tattatcacc acttcgtcaa tccaggcata ccagccaagc
    13621 ccgcatttac tggactatgc agctacgaag gcggcgattc tgaactacag ccgaggcctg
    13681 gcaaaacagg tcgcggagaa aggtattcgg gtgaatattg tcgcgccagg cccgatctgg
    13741 acagcactgc aaatttccgg cggacaaacg caggataaga tcccgcagtt tggtcagcaa
    13801 acgccgatga aacgtgcggg gcaaccggcg gaactggccc ctgtatatgt ttatctggca
    13861 agtcaggagt cgagctacgt caccgcagaa gtgcacggcg tgtgcggcgg cgagcattta
    13921 ggttaaaaaa atgcccggtt gtgaaaagca accgggcatc attgtgaatt cactcttacc
    13981 tgatgtggcg aaaacgcctt atccgcctac aaatgcatga agattcaaca tgttgcaata
    14041 gattttgtag gcctgataag cgtagcgcat caggcattga gcacctaatg ccggatgcgg
    14101 cgtgaacgcc ttatccggcc tacaaacgca cgaagattca acatgttgca atagattttg
    14161 taggcctgat gaacgtagcg catcaggcaa ttctacttta cttcgctttg gcggtttctt
    14221 cgccgaccag acctatcttc aggtaacccg cctgatgcag cgtgtccatt accttcatca
    14281 acgtctcgta atcgacagtt ttatccgctc ggaagaagat ggtggtgtct ttcttgcctt
    14341 cggttaacgc attcaacgcc gtaatcattg tttcatcggt gaccggatcg ttaccgataa
    14401 acatcgagtt gtctgccttc accgacagat aaaccggttt ttccgggcgc ggctgcggcg
    14461 tgctggtaga agcaggcaag ttaaccttca catctaccgt cgctaacggt gccgccacca
    14521 taaagataat cagcaggacc aacatcacgt cgataaacgg cgtcacgttg atatcatgca
    14581 tttcgccgtt atcgtcgagg ttttcgttaa gatgcattgc catcggacat tatcctgcgc
    14641 gtaatttttg tgcgacacga accggatgcg cagcggcgct ggcttccaga tccaggtcac
    14701 ggctttgcag caacaatacc tgcgctgcga catcacccag catcgcttta aagccgccaa
    14761 tctgacgcgc gaatacgtta tagataacga ccgccggaat cgctgccacc agaccgattg
    14821 ccgttgctaa cagagcttct gcgatacccg gcgcaacgac tgccaggtta gtggtctgcg
    14881 tttgcgcgat accaataaag ctgttcatga tgccccagac cgtaccaaac agcccaacga
    14941 acggcgaaat cgcgccgatg gttgccagat agccgttacc gcggcccatt tgacgaccta
    15001 ctgcggcgac ccgacgctcc agacggaagc tggtacgttc tttaatacct tcgttatcgt
    15061 cgctgccttc tgacagttcc agctcattct gcgcttcatt gagcaaatgc aggcttaagc
    15121 ttttgctacc aaaatcagcg gcgatatcgt tggcctggtt taaggaacgc gcttcagcca
    15181 gcagttgctg ctcgcgctta aggcgacgct tctgattaaa gaactctacg ctcttactga
    15241 agaagattgc ccaggtgact acggaggcca aaataagccc aatcatcacg cacttaacga
    15301 caatatcggc gtgctgatac ataccccaga cggaaaggtc cgtctgcatt aaattattac
    15361 ccactgtgta tctccaggac gcaagtcaca aaatctgcgc ataataatat caaaacgacg
    15421 tcgaattgat agtcgttctc attactattt gcatactgcc gtacctttgc tttcttttcc
    15481 ttgcgtttac gcagtaaaaa agtcaccagc acgccatttg cgaaaatttt ctgctttatg
    15541 ccaattcttc aggatgcgcc cgcgaatatt catgctagtt tagacatcca gacgtataaa
    15601 aacaggaatc ccgacatggc ggacaaaaag cttgatactc aactggtgaa tgcaggacgc
    15661 agcaaaaaat acactctcgg cgcggtaaat agcgtgattc agcgcgcttc ttcgctggta
    15721 tttgagagta tggaagccaa aaagcacgcg acgcgcaatc gcgccaatgg cgagttgttc
    15781 tatggacggc gcggaacgtt aacccatttt tccttacaac aagcgatgtg tgaactggaa
    15841 ggtggcgcag gctgcgcgct atttccctgc ggggcggcag cggttgctaa ttcgattctt
    15901 gcttttgtcg aacagggcga tcatgtgttg atgaccaaca ccgcctatga accgagtcag
    15961 gatttctgta gcaaaatcct cagcaaactg ggcgtaacga catcgtggtt tgatccgctg
    16021 attggtgccg atatcgttaa gcatctgcag ccaaacacta aaatcgtgtt tctggaatcg
    16081 ccgggttcca tcactatgga agtccacgac gttccggcga ttgttgccgc cgtgcgcagt
    16141 gtggtgccgg atgccatcat tatgatcgac aacacctggg cagccggtgt gctgtttaag
    16201 gcgctggatt ttggcatcga tgtttctatt caagccgcca ccaaatatct ggttgggcat
    16261 tcagatgcga tgattggcac tgccgtgtgc aatgcccgtt gctgggagca gctacgggaa
    16321 aatgcctatc tgatgggcca gatggtcgat gccgataccg cctatataac cagccgtggc
    16381 ctgcgcacat taggtgtgcg tttgcgtcaa catcatgaaa gcagtctgaa agtggccgaa
    16441 tggctggcag aacatccgca agttgcgcga gttaaccacc ctgccctgcc tggcagtaaa
    16501 ggacacgaat tctggaaacg agactttaca ggcagcagcg ggctattttc ctttgtgctt
    16561 aagaaaaaac tcaatgatga agagctggcg aactatctgg ataacttcag tttattcagc
    16621 atggcctact cgtggggcgg gtatgaatcg ttgatcctgg caaatcaacc agaacatatc
    16681 gcagccattc gcccacaagg cgagatcgat tttagcggga ccttgattcg cctgcatatt
    16741 ggtctggaag atgtcgacga tctgattgcc gatctggacg ccggttttgc gcggattgta
    16801 taacattgcc acttttggac aattttgcag acattttatt gtgaaaagtc ttaaattgtt
    16861 gcgtccggga tcaaggcgtc ccggacgatt caggagtaca ataggcagat aaaggcttaa
    16921 acgctgttcc acaggaaagt ccatggctgt tattcaagat atcatcgctg cgctctggca
    16981 acacgacttt gccgcgctgg cggatcctca tattgttagc gttgtttact ttgtcatgtt
    17041 tgccacgctg tttttagaaa acggcctgct gcccgcctca tttttgccag gcgacagctt
    17101 gttgatattg gctggcgcat tgattgccca gggggttatg gattttctgc ctacgattgc
    17161 gattctgacc gccgcagcaa gtctgggctg ctggctaagt tatattcagg ggcgctggtt
    17221 agggaatacc aaaacggtga aaggctggct ggcacagctt cctgctaaat atcaccagcg
    17281 cgccacctgc atgtttgacc gccacggtct gctggcgctg ctggctggac gttttcttgc
    17341 atttgtccgt acgctgctgc caaccatggc gggaatttcc ggtctgccaa accgccgttt
    17401 ccagtttttc aactggttaa gcggattgct gtgggtcagc gtggtaacca gtttcggcta
    17461 tgccttaagt atgattccgt tcgttaaacg ccatgaagat caggtaatga cgttcctgat
    17521 gatcctgcca attgccttgt taaccgctgg cttgttaggc acgctgtttg tggtgattaa
    17581 aaaaaaatac tgtaacgcct gacgattttc cctgttcccg gttgctgtac cgggaacgta
    17641 tttaattccc ctgcatcgcc cgcattcttg ccgcatcttc ccccggcgtc acaccgaagt
    17701 aacgtttaaa ctcacggcta aattgcgatg cgctttcata gccgacgcgc atcgctgctg
    17761 cgctggcctt catgccgtca tggatgatca tcatccgcgc cttatgcaga cggtaattct
    17821 tcaaatactg caacggcgag gtgctggtga cagacttaaa attatggtgg aacgccgata
    17881 cgctcatatt agcttctgcc gccagttgct cgacgctcag gttttcggtg tatttattct
    17941 caatccgttt cagcacgcgg ctaatcagac tgaagtgagt ctggcgactg accagcgcca
    18001 gtaacgcgcc gccgcaaggt ccggtcagca cgtagtacag aatttcgcgg atgatctgtt
    18061 tgccgagaat acgcgcatcc agtggtcgcg ccatcacgtc gagtaaccgc tccgccgcgc
    18121 ataaaatctc ttctgataac gtggcggagt taatcccgct ggctgccatc gacggctgga
    18181 aatgctcatc ttcgccaata tccatcaaca gttcctgtaa ctgcaaaata tcgacattga
    18241 gacgcaaccc tgccagcggc acctctgacg tggcataggt ttcgcactca aacggcaacg
    18301 gcaccgtcag cagcaggtat tcattggcat cataacgaaa cacgcgttca ttgatataac
    18361 cgattttatg tccggaaaag agaattatga tgccaggctc gtacatcacc ggtgtacgtg
    18421 cgaaaggcgt ctcgccatac aacaaacgca catcgggcaa cagttctgac aaactatttt
    18481 ctttattttt cagtttatta actttatccg ccagcaagcg gcaaatctct tcacgtttca
    18541 tatcgcgtaa tttcttagga ataatgcggc aatttgattg tgcgcaattt tgtagcattt
    18601 ctccagcact ctggagaaat aggcaagaca ttggcagaaa tgagcattga gagccagggc
    18661 gctggcgatc acaatgaaaa acatcaggca gatcgttctc tgccctcata ttggcccagc
    18721 aaagggagca agtaatgaac aactttaatc tgcacacccc aacccgcatt ctgtttggta
    18781 aaggcgcaat cgctggttta cgcgaacaaa ttcctcacga tgctcgcgta ttgattacct
    18841 acggtggcgg cagcgtgaaa aaaaccggcg ttctcgatca agttctggat gccctgaaag
    18901 gcatggacgt gctggaattt ggcggtattg agccaaaccc ggcttatgaa acgctgatga
    18961 acgccgtgaa actggttcgc gaacagaaag tgactttcct gctgggggtt ggcggcggtt
    19021 ctgtactgga cggcaccaaa tttatcgccg cagcggctaa ctatccggaa aatatcgatc
    19081 cgtggcacat tctgcaaacg ggcggtaaag agattaaaag cgccatcccg atgggctgtg
    19141 tgctgacgct gccagcaacc ggttcagaat ccaacgccgg tgcggtgatc tcccgtaaaa
    19201 ccacaggcga taagcaggcg ttccattcag cccatgttca gccggtattt gccgtgctcg
    19261 atccggttta tacctacacc ctgccgccgc gtcaggtggc taacggcgta gtggacgcct
    19321 ttgtacacac cgtggaacag tatgttacca aaccggttga tgccaaaatt caggaccgtt
    19381 tcgcagaagg cattttgctg acgctgatcg aagatggtcc gaaagccctg aaagagccag
    19441 aaaactacga tgtgcgcgcc aacgtcatgt gggcggcgac gcaggcgctg aacggtttga
    19501 ttggcgctgg cgtaccgcag gactgggcaa cgcatatgct gggccacgaa ctgactgcga
    19561 tgcacggtct ggatcacgcg caaacactgg ctatcgtcct gcctgcactg tggaatgaaa
    19621 aacgcgatac caagcgcgct aagctgctgc aatatgctga acgcgtctgg aacatcactg
    19681 aaggttccga cgatgagcgt attgacgccg cgattgccgc aacccgcaat ttctttgagc
    19741 aattaggcgt gcctacccac ctctccgact acggtctgga cggcagctcc atcccggctt
    19801 tgctgaaaaa actggaagag cacggcatga cccaactggg cgaaaatcat gacattacgc
    19861 tggatgtcag ccgccgtata tacgaagccg cccgctaagc tttttacgcc tcaaactttc
    19921 gttttcgggc atttcgtcca gacttaagtt cacaacacct caccggagcc tgctccggtg
    19981 agttcatata aaggaggaac gtatggctaa tccaaccgtt attaagctac aggatggcaa
    20041 tgtcatgccc cagttgggac tgggcgtctg gcaagcaagt aatgaggaag taatcaccgc
    20101 cattcaaaaa gcgttagaag tgggttatcg ctcgtttgat accgccgcgg cctataagaa
    20161 tgaagaaggt gtcggcaaag ccctgaaaaa tgcctcagtc aacagagaag aactgttcat
    20221 caccactaag ctgtggaacg acgaccacaa tcgcccccgc gaagccctgc tcgacagcct
    20281 gaaaaaactc cagcttgatt atatcgacct ctacttaatg cactggcccg ttcccgctat
    20341 cgaccattat gtcgaagcat ggaaaggcat gatcgaattg caaaaagagg gattaatcaa
    20401 aagcatcggc gtgtgcaact tccagatcca tcacctgcaa cgcctgattg atgaaactgg
    20461 cgtgacgcct gtgataaacc agatcgaact tcatccgctg atgcaacaac gccagctaca
    20521 cgcctggaac gcgacacaca aaatccagac cgaatcctgg agcccattag cgcaaggagg
    20581 gaaaggcgtt ttcgatcaga aagtcattcg cgatctggca gataaatacg gcaaaacccc
    20641 ggcgcagatt gttatccgct ggcatctgga tagcggcctg gtggtgatcc cgaaatcggt
    20701 cacaccttca cgtattgccg aaaactttga tgtctgggat ttccgtctcg acaaagacga
    20761 actcggcgaa attgcaaaac tcgatcaggg caagcgtctc ggtcccgatc ctgaccagtt
    20821 cggcggctaa cgtgcaaatt ctcccggtgg cggtaatgtt ccgctaccgg acttttcaga
    20881 aatcatttat tcccctcgcg tcccgcccgt tgttactctt ccttgttcag gaatgccaaa
    20941 tataaggaca tcatcatgca gagccggaag ctcttaaaag aacaactcat ctatatccgg
    21001 gataaacgca acggagaggt gaaaaacaga tgaaaataat acttctgttt ttagcagccc
    21061 tggcaagttt taccgtgcac gcacagcccc cctcacagac cgtagaacaa acagtccggc
    21121 atatttatca gaactataaa tcagatgcca ctgcccctta ttttggtgaa accggagagc
    21181 gtgcgataac ttctgtgcgt attcaacagg cgcttaccct gaacgacaat cttacgctgc
    21241 cgggtaatat tggctggatt atgatccggt ttgtgattgt caggattttg gcgatctggt
    21301 gctagaaagc gtagcgataa ccgaaactga cgccgatcat gccgatgccg ttgtgcgctt
    21361 tcgtatcttt aaagatgata aagaaaagac cacgcagaca ctgaaaatgg tggcggaaaa
    21421 tggtcgttgg gtcattgacg atattgtcag caatcatggc agcgtcttac aagcagttaa
    21481 tagcgagaat gaaaaaacgc tggccgcttt agcttcgttg caaaaagaac agccggaagc
    21541 ctttgttgcc gaactctttg aacatattgc cgattatagc tggccgtgga cgtgggtggt
    21601 ttccgactct taccgccagg cggtcaatgc cttctataaa accaccttca agacggccaa
    21661 caatcccgat gaagatatgc aaatagaacg gcaatttatt tacgacaatc cgatctgttt
    21721 tggcgaagag tcgctatttt cacgcgttga tgaaattcga gtcctggaga aaaccgccga
    21781 ttccgcccgc attcatattc gttttacgct gaccaatggc aacaacgaag agcaagaact
    21841 ggttttacaa cggcgcgaag gtaagtggga aatcgctgat tttatccgcc cgaacagcgg
    21901 cagcctactt aagcagattg aggcaaaaac cgccgccaga ttaaagcaat gagctgaatt
    21961 aaatagcaat tagccggaac aataaaaaag ggaacactat atgaaaacga ttttcaccgt
    22021 gggagctgtt gttctggcaa cctgcgtgct cagtggctgc gtcaatgagc aaaaggtcaa
    22081 tcagctggcg agcaatgtgc aaacattaaa tgccaaaatc gcccggcttg agcaggatat
    22141 gaaagcacta cgcccacaaa tctatgctgc caaatccgaa gctaacagag ccaatacgcg
    22201 tcttgatgca caggactatt ttgattgcct acgctgcttg cgtatgtacg cagaatgata
    22261 aaaaaatccc cggcagcatg tcagttgccg gggatttttt ttaacgtcca accgccgctt
    22321 tagggcgttt cttcgcacca gcattcaccg gacgagattg cgtagacgac gctttttttg
    22381 ccgtagcagg cgtctgacac tgggtcgcca tcggcgtatg tttcgtcaac gccggacggg
    22441 tattgcggtt ctggcgacga gcctcacgca tctcttcaat ggttggcgca ggcactaagc
    22501 aatcgcgacg gctgccaatc agatgctttt tgcccatcgc ttccagcgcc tggcggatta
    22561 acggccagtt tgccggatcg tggtaacgca acaacgcttt atgcaaacga cgctgtttgt
    22621 cgcccttcgg tacgaagacg tcttcactct tataaccaat cttcgccagc gggtttttcc
    22681 cggtgtaata catggtggtc gagttagcca gtggcgatgg gtagaagttc tgtacctggt
    22741 cgagacggaa acgatgcttt ttcagccaca gcgccaaatt caccatatct tcatcacgcg
    22801 taccggggtg cgcggagatg aaatagggga tcagatactg ctctttacct gcctgtttcg
    22861 agtaggtatc gaacagctct ttaaagcggt cataactgcc catgcccggc ttcatcatct
    22921 tcgacaacgg cccttcttcg gtatgttccg gagcaatctt cagataaccg ccgacgtgat
    22981 gggtcgccag ttctttgata tagcgcggat cttccacggc gatgtcataa cgtacgcctg
    23041 aggcaatcag aatcttttta atgcctttca gatcgcgagc gcggcgatag aggttgatcg
    23101 tcggttcgtg gttagtgtcc atatgcggac aaatatccgg gtaaacgcag gacaaacgac
    23161 ggcaggtttg ttcagcgcgt ggcgatttgc agcgcaacat atacatgttg gcagttggcc
    23221 caccgagatc ggaaattacg cccgtaaaac ctggaacggt gtcgcggatc gcttcgatct
    23281 cattaatgat cgaatcttcg gaacggctct gaataatgcg cccttcgtgc tcggtgatag
    23341 aacagaaaga gcagccgcca aagcagccgc gcataatgtt gaccgaaaaa cggatcattt
    23401 cgtaagccgg aatacgggca ttgccatagg ccggatgtgg cacgcgcttg tacggcagcg
    23461 caaaaacgct gtccatctct tcggtagaaa gcgggatagc aggcgggttg atccacacat
    23521 agcggtcacc gtgtttttgc atcaatgcgc gagcgcaacc tgggttagtt tcgtggtgca
    23581 gaatacgcga agcatgggcg tacagcactt tatcgccctt cactttctcg aaagaaggca
    23641 gcaacacgta ggttttttcc cacggtttcg ggcgcggtgg ctgcacggtt acggctttgg
    23701 cttcctgctt tttcggtgcc accggtttgt tatccgcgca cggcaaatct tcaccatacg
    23761 gatgcgggat tgggtcgatt tttccagggg tatcaagacg ggtggaatcc acgccgctcc
    23821 agccaggcag cgcctctttc acgataatcg cggtattacg cacatcgcgg atttcactaa
    23881 tcggctcgcc catcgccaga cggtgcgcca cctccaccag cggacgctca ccgttaccaa
    23941 acatcagcat gtcggctttc gaatccacca gcacggaacg gcgcacggta tcggaccagt
    24001 aatcataatg cgcggtacgg cgcagactag cctcaatacc accgaggatc accggtacat
    24061 ctttccacgc ctctttacaa cgctgggtat aaaccagtgt ggcgcgatcc gggcgcttac
    24121 ccgcgacgtt atccggcgtg taggcatcgt catgacgtaa acggcgatcg gcggtataac
    24181 ggttgatcat cgaatccatg ttgccagcag taacaccgaa aaacagattc ggtttaccca
    24241 gacgcatgaa atcgtctttg ctgctccagt ctggctgggc gatgatcccg acgcgaaagc
    24301 cctgtgcttc cagcatacga ccgcaaatcg ccatcccgaa gcttgggtga tcgacatacg
    24361 cgtcgccagt aaccaaaatg atgtcgcagc tatcccagcc aagttgatcc atctcttcac
    24421 gagacatcgg caaaaacggt gccggtccaa aacaggcggc ccagtactgc ggccaggaga
    24481 acaggtcgcg atccggttgg atcagggaga tagagctcat tttgcttcca gaaatgataa
    24541 aaaaataatc aaaggccggg gattataagc cggaacgaaa gagaaatcga aaagtattcc
    24601 atactcgccc tcctcgggcg agtatgaaga ttacggtacc ggattgacta acagttgccc
    24661 aatcgagcca cggtccgcca tttccagcgt ctgactgttg aagtagaacg ggaagtgcgc
    24721 ccaggaaggc tgaccgaaat aaacaagcag ctccacttgt ccgtcaaccc aaacggtatc
    24781 tttccagcct ctgtcttccg ggaacggcat cgcaccattc acgttacgga tctggaacat
    24841 cacgccttca atatggaacg cttgcggctc gtccgcgcgt accgtccagc gttcccacgt
    24901 tccttgctgc gcggtgacat caatacggtt gacgtcccac agttgtccat taatacccgg
    24961 gtcatcaccc agactgatat cgcgactgcg aatcggcgaa ccggccatga tttcagttgg
    25021 cagcaagcgc atcggaagac tgtctgtgac cagcggcaga aggccggttg ggcgcagcgt
    25081 tagcaccagg gtagaaacca gaatgctgga tggctcaaag aagccacgaa tacgatcaac
    25141 aatgctcgcc gcttcgccac aggtgatcga cacttcatca ccgttgctca tatccaccag
    25201 aatctcgcgg cgctcgcccg gtgccaacga aagttgcttc accgatacag gagcaggcag
    25261 gaatccctga tcgccagaaa tcacatgtaa cgggcgacca tcgctcatct gtagttgata
    25321 gcgacgagag ttcgacgcgt tcagcagtcg caagcgcacc cagccacgcg agacttcaac
    25381 gtacgggctt tgtacaccgt taaccagcag cgtatcacca acaaagccgc cgcttcccgg
    25441 ttcgttgtat tctggcgtac caaagttatc cagccgttta tcctggataa tgaccggaaa
    25501 atcatccaca ccataatggt tggggatagg cagcgacttg ctgacttcat cttccaccag
    25561 ccacattccg gcaaggccgt tatagacctg ctgagccgtg cggttgggag tattggcgtg
    25621 ataccacaga gtagctgcgt tctggcgaat aggcagtacg ggtgcccagt cagcgtttgg
    25681 cgacatcatc cgtgccggac cgcccatcag cgggcctggt acctgtagcc cggccaccgt
    25741 cattgagaca ttttctgtca ggcggttgct gtaaataagc ttaacatcgt cgcccttcca
    25801 gacgcggata gtcggcccca ggtaacgacc attgattccc cagaccgacg cgcgtgtccc
    25861 tggcgtaaat gaccagtgcg cacgttgtac agtcataaac agcggttgcc cacggcgaga
    25921 ttcaagcagc ggcggaacgg gtagcggttg ctgttgcccg gctgcgctgg ccttcagggg
    25981 aacagcgcct gcacaaagtg caatccccga tgcctgaatg aactgacgcc gactgagtga
    26041 catatttgct ccatgtaaaa ctggctaaag taataccgtg caggaaagtg tttcccgcaa
    26101 aaatacagat tgccgactta aacttttccg gcggcttcgc gctctgcgac ttctttatcg
    26161 agctcggcga ttttttgttc cattatcgaa cgacaatggg cagccagctc acgaacctga
    26221 tctttgccat actgactgac gtcaattggc ggcagcattt cgacaatcac cagaccgttg
    26281 tgcagtcgat taagattaat cttattcgaa gttgtggaga cgcacacggg aataatcggg
    26341 acgcccgccg caattgccgc gtgaaatgcc ccagtcttga acggtagcag gccgcgacca
    26401 cggctgcggg taccttccgg gaacatccag atggaaatac ggcgtttttt gaaatgattc
    26461 actacttccg caatagtgcc gtgagcttta gtgcgattgt ttctgtcgat caataagttg
    26521 ccggttaacc agtacaactg cccgaagaag gggatccaca gcaagctctt tttacctacc
    26581 gtcaccgtcg gcggttgcac aatgttcgat gctgtcacca tgtcatagtt gttctggtgg
    26641 ttagcgatat agatagcatt gccgtagctt tcagcatctg caggtttgcg gcactcaact
    26701 ttcaggccga acagcggcgc aagacgacca aacatatgcc caaaggtggc cacatgtttc
    26761 gggttacgcg ggctgaaaag gcagtaaatg gagccgaata cacagactaa gatgctgtaa
    26821 atcacggtaa taataagacg aaagatatat agcataacac cctctgaagg tctgagagac
    26881 tggcattgta cgtcacctga aatcaggtta gggcattgtt aaaagccgct ctctggaaaa
    26941 gcgtattgtt aatcgcaacg catgaataaa caacggtttc aagggaattt ttatcgtttc
    27001 cgggggttga atacgctgcc ggatgacgac ttaacgtttc atccggcgtt ccttgcaagc
    27061 gggaggaaac agcgccctcc ccggcatatt actcttcgct atcaccgctg ctggcacggc
    27121 gaggagagtc gatctcaaca cgatcgatac gctgcaaacc gcgcatcaac gtaccgcggc
    27181 gtccacgttc gccagtgact ttctgtagct cttccggacg cagtttaatt ttgcgtttcc
    27241 caacatgaat ggtcagcgtg ctttgcggcg gcagaacgta cagttgcgcc agaccatcct
    27301 cgccacgcgc ggcttctgcc gatggaatgt tgataatctt gttgcctttg cccttcgaca
    27361 gctgcggcag atcacttacc gggaacatca acatacggcc tgcctgagtg attgccagca
    27421 gcatatcgga agcatcttca attaccaccg gcggcataac atgggcattt tccggtaagg
    27481 tgatcaaagc cttacctgca cggttacgcg ccaccagatc gttaaaggtg cagacgaaac
    27541 cgtaacccgc atcggaagcc atcagcagtt tctgatcgtc gctttccatc agcatatggt
    27601 caacggtcgc cccaggcggc aacgttaatt tgccggtgag cggttcgccc tgaccacgcg
    27661 ccgacggcag cgtaatcgga tcaatggcat agctacgacc ggtggaatca acaaacacta
    27721 ccggttggtt gctcttacct ttcaccgccg ctttgaagct atcacccgct ttataattca
    27781 ggcccggcgc gtcgatatca tggcctttag cgctgcgtac ccagcccatc tgcgacagca
    27841 caatggtgac aggttcagac ggcagcatgt cgtgctcgct catcgctttc gcttcttcgc
    27901 gttcctgcaa cggcgaacga cgatcgtcac cgtaggcttg cgcgtctgcc tgcagttctt
    27961 tcttcagcag gttattcatt ttacgctcgg aagccaaaat gccctgcaac tggtcgcgct
    28021 ctttttccag ctcactctgc tcaccgcgaa tcttcatctc ttccagtttg gcaagatgac
    28081 gcagtttcag ttcgaggatc gcttccgcct gggtttccgt aaggccaaac cgcgacatca
    28141 gcgccggttt cggttcatct tcattacgaa tgatctcaat cacttcgtcg atattgagaa
    28201 acgccaccag caaaccttcg aggatatgca ggcgcttgag gactttctcc agacgatagt
    28261 tcagtcggcg gcgcacggta tcgcggcgga acaccagcca ttcggagaga atttccagca
    28321 ggtttttcac cgccggacga ccatccagac cgatcatatt aaggttaatg cgatagctct
    28381 tttccagatc ggtggtagcg aagaggtggt tcatcacctg atccatatcc acgcggttgg
    28441 aacgcggcac aatcaccaga cgggtcgggt tctcgtggtc agattcatcg cgcagatcgt
    28501 caaccatcgg cagctttttg ttgcgcattt gcgcagcaat ttgctccagt acgcgcgcac
    28561 ctgaaacctg atgcggcaat gcgctgataa ccaccgcgcc atcttctttc ttccacaccg
    28621 cgcgcatacg cactgaacca cgtccgttct cgtagatttt acggatctcg gcgcgcgaag
    28681 tgataatttc cgcttcagtc ggataatccg gcccctgcac gatatccagc agctgatcga
    28741 gcgtggtttt cggctggtcg attaatgcga ttgccgcctg agccacttca cgcaggttat
    28801 gcggtggaat atcggtcgcc atgccgacgg caataccggt ggtgccgtta agcaaaatgt
    28861 ttggcagacg ggcaggtagc attttcggct cctgcaaagt gccgtcgaag tttggcaccc
    28921 agtcagccgt cccctgcccc aactcgctca atagcagctc ggaatatttc gacaaccggg
    28981 attcggtgta acgcattgcc gcgaacgatt tcggatcgtc cggcgcgccc cagttcccct
    29041 gaccatcaac cagcggataa cggtaagaga acggctgcgc catcaggacc atcgcttcat
    29101 aacaggcgct atcgccgtgc ggatggtatt tacccagtac gtcaccgacg gtacgggccg
    29161 attttttaaa tttggcgctg gcattcaggc ccagttcaga catcgcatac acaatgcggc
    29221 gctgaacagg tttcagacca tcaccaataa acggcaacgc acggtccatg atcacgtaca
    29281 tggagtagtt taagtaggcg ttttccgtaa attcatgtag cgcaaggcgc tctgccatat
    29341 cgctcattaa ttctgattcc tcaacttatt cgccagcctt caaaggggca atattgccgg
    29401 agatactaca ttatctgccg cgtcgagtca caggagatca gcgtgaatag cgccgccgga
    29461 cgtgctccga cggcgataaa ggcatcaatg tgccttgata tacaactctt tgctgtagac
    29521 ataatcctgg ggattatgca gcggaaaacc gccaacgtag ggtttaagca gtttgattaa
    29581 cggctgatag tagataggaa tcagcggtgc ctgctggttg acgatcactt ctgcctgctg
    29641 atacaacgca ttacgctttg tcgcatcagt gatctgcatg gcctggttta gtaaggcgtc
    29701 atactgcgca tttttccagt gaccgacgtt ttcttcacta tcgcttttga gcgtgttcag
    29761 gaagctggaa gcatcattgt acgtcgcatc ccacgactgc cgagacagca tgaaatcacc
    29821 ggctcgtcgg gcatcaagat aggttttcca ctccattgtg cgcagcgtca cctgtgcacc
    29881 cagccatttt ttccattcgg aagacaacgc tatcgcggtc ttttcatgca gatcgtactt
    29941 gttgtagaac agctcaaagc gtagcggatg agaggcgtcg tatcccgcct gtttcagcaa
    30001 ggcttttgcc atcgcgacgc gctcactcat tggcttttgc agttcatcga acgtcgtcgc
    30061 gctaaagcct tttacctctg gcggcgtcag cgtggttgcg ggcgttctca accccagtac
    30121 cttttgcgca ataagctgtc gatcaaccgt aagatatagc gcccgacgca ctcgcacatc
    30181 gttaaatggc ggtttctcaa ggttgaagtt gtaatattcg ctgttcagac gcggaataat
    30241 tcgtatctcg ccaggcagtg atttttcaat ggcgggaatt tgctgcgccg gaacccaggt
    30301 gagatcgacc tctcccgcgc gatagcggtt atagccggtg accgaattat ccagcgccag
    30361 atactcaacc tgttgcaata ctgtatgttg cgcatcgcgg tactttggat ttttgcgtgc
    30421 agtaatcttt tcgttaacta cccactgatc aagcacaaag gcaccgttgt aaaccatgtt
    30481 ctctggctta ctccagctat cgccatgttt agcgatgaca tgatgaggaa ccgggaacag
    30541 cgtcggccag gcgagcatcg tcgtgaacca aggaaccggc tgctcaagcg taacttcaag
    30601 agtacgatca tccgtcgctt tgacacccag cgatgtaaca tctgctttac ccgcaacaat
    30661 agccgcggca ttgttaatgt gcgcctgtgc cagatagcca gcaaaagggc ttgccgtttt
    30721 cgggtcaacc gcgcgctgcc agccgaggac aaaatcctct gccgtcagag gctgaccgtc
    30781 ggaccactgc aaaccgctac gcagatggaa aatatagcgc ctgccgccgt ccagtatctc
    30841 ccagcgttca gcctgagcgg gctgcacctg gccttcaccg tccatccata ccagaccttc
    30901 aaacagatcc agcacaatct gcgcggcagt attctcctcc accttttgcg ggtcgagcgt
    30961 acctgggtcg ctatgattgt tgtaacgaaa gacttgttgc ggggcgagct gtgtgttggt
    31021 gggaacgtca gcagcgtaga gaggagccgc acttaccagg ctgaccagcc ataataaatt
    31081 tcgcgtatac atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg
    31141 gcattgttcg ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca
    31201 tcacgctaac ggcacataaa cattcgtttc cagtcgctgc tccgtcaccc cttcggcaag
    31261 attggtgtag tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca
    31321 gtggcgaata atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgagc
    31381 cacggcataa cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat
    31441 cggttcgcta acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg
    31501 gtcgtcccag gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt
    31561 acgccacatg ataaatttcg ccacgctgta gttgagcaac tcaggcctac agcggtgcgg
    31621 caacatcgcc acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt
    31681 catgttgcct ccctgcgaaa ttattcccgg gaacgcatcc cgggagcggt aacaattacg
    31741 gatttacttt gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt
    31801 caccctgaat ctcaaccgta tctttcggcg tcaccgtcac gccgttccag cgtttgtggt
    31861 cgatatcaac attgatagta ccgctggcat ctttgaacac gtagagatcg tcagagatgc
    31921 gttcaacgat attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt
    31981 ctacagtcgt tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg
    32041 atgggccaga aaaaccgccc tgctctgctg ccatcaccgg tgcgctgcac agggccatta
    32101 ctgcgattac tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag
    32161 catttaacaa gatagtcctt aacaacttct taagggaaaa aaataaaatt tagtgctgta
    32221 cagagcgcgt tacaacacgg tttactagca gcaaatacgg ttatcgcagg gatgaaaaaa
    32281 tgcgaatttt actgatagaa gatgacatgc tgattggcga cggcatcaaa acgggcctta
    32341 gtaaaatggg ttttagcgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt
    32401 atagcgcacc ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg
    32461 atattttgcg cgaatggcga gaaaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc
    32521 gcgatgcgct ggaggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta
    32581 aaccttttgc gttgatagaa gtcgccgcca ggctggaagc tctgatgcgc cgaaccaacg
    32641 gccaggccag caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg
    32701 ccacgctggc tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac
    32761 tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct
    32821 gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac
    32881 tcggtagtga ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa
    32941 atttacccaa cgtcttagtc tgcgtgtcag gctgacgcta atctttttaa ttctggcctc
    33001 ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga
    33061 attgttcgac acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga
    33121 aatcaacgcg gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga
    33181 tgacgatgcg ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaacgatgg
    33241 cgataacgga gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact
    33301 ggtgggcgat aaagatcaat ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg
    33361 catcgttgtt ggtcaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg
    33421 gcaattgatc ccgtggttgg tcgcactgcc ggttatgtta atcatcatga tggtactact
    33481 gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga
    33541 ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc
    33601 gctaaatcaa ctgttcgccc gcacacatgc gatgatggtt cgtgaacgac gctttacctc
    33661 cgacgcagct cacgaacttc gtagcccgtt aacggcgctg aaagtgcaaa ccgaagttgc
    33721 gcagctctct gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg
    33781 gatcgatcgc gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact
    33841 ggataacctt caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat
    33901 ggatatttac cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgtcca
    33961 gggcatcaaa cgcaccggac aaccgctatt gctaagtttg ttggtgcgaa atttgctgga
    34021 taacgccgtg cgttacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa
    34081 tttcatcgta agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga
    34141 acgcttctat cgcccacccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt
    34201 ccagcgaatc gccaagctgc atggcatgaa tgttgagttt gggaatgcgg aacaaggtgg
    34261 atttgaggcg aaggtaagct ggtaagggta agacttttgc taaattcgca aaagactttg
    34321 cacattttgc taatttcacc gtaccgctct gtgacgtact atagtcggca aacgtctcac
    34381 cttgaggtta aaaaatgagc aacatcctga ttatcaacgg cgcgaaaaaa ttcgcccact
    34441 ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg
    34501 ggcatgatgt tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa
    34561 actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt
    34621 ggacagtgaa aaaatacatt gatgatgtat tcaccgaagg tcacgggact ctgtatgcca
    34681 gcgatggtcg tacccgcaaa gatccgtcga aaaaatacgg ttccggcggc ctggtacagg
    34741 gcaaaaaata tatgctttct ctgacctgga acgcgccaat ggaagccttc accgaaaaag
    34801 atcagttctt ccacggcgtt ggcgttgacg gtgtgtatct gccgttccat aaagcaaacc
    34861 aattcctcgg tatggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg
    34921 atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag
    34981 agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc
    35041 ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac
    35101 tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt
    35161 tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcatc
    35221 ttgaagcgca tctgcaaacc ccgcacatga aggcgtatag cgaagccgta aaaggtgacg
    35281 tgctggagat gaatattcgt attctgcagc cagggattta acattcctga aaagaggccg
    35341 gtgttgctac cggcctcagg tcagattagc cattctttct tcccgttttc agcccccgat
    35401 ggaaatcgtt aattcgctta atggttttaa ccgcacgctg cggcaccgcg gaagccaccg
    35461 accagacaat catttccagg cacaacaata ccgtaccatg tagtggcgtt ttcccctttt
    35521 catccccgcg cggaacatga atcacgatac tggcgtcttt gctaaaacgt gaatccagcg
    35581 cgtttgtcag caaaataaca gggatcccca gccgtctcgc ttcacgcagc gttgtcagcc
    35641 cttcccggtg cgcagatttt tgcgccatca tgatcagaac atcgccacgt tgaagcgcaa
    35701 tcagttgctc ggcaaggcca atacccgtac ggtttagtgc cgtggcaggc aaacctatgc
    35761 ggttgaatag tcgtgcggta tagtccgcca gaatacccga agcgccaatg ccaaaaattg
    35821 cgacttgccg tgcctgtacc aacaacgcta ccgcttgcgc aagcgcgtgg cggttttcag
    35881 gctctgacag gacattacag gtatacaaat gcccttcaag aacaaagtcg attgcggtgt
    35941 taacatcgct cgtcaagtta ctcaccgtcg tagacatctt ttcgctggaa ctaagggctg
    36001 gaccaagcca ctgttccaga gtgcgtttca gatcacgcag cccggcaaac cccagggcct
    36061 gtatagccct gataactgtg gcatcggaag tgttcaacgt ggccgcaatt tccatcgccg
    36121 tttgatcgag taccacttca cgatgctcat taatatagct ggcgacagct cttaacccag
    36181 gcgttaactg actcgcgcga gtgcgaaaac gctcaccata aatatcaacc cgcgttttgt
    36241 ctttctgaat catttggcct ccggtaaagg tcgccccgca atttgtgatt gtatctgcgc
    36301 agccatcagc cccagagatg caaaggagtt agaaattgcc tcttcacgag aaatcagcac
    36361 ataacgcccg gaacgacagg cagtcagaaa ctgacaccat cccggcatga ctttttccat
    36421 tgctgccagc tcatcctgag gtttaccacc ggtgtcaccg cgccaggttg caaaaacaaa
    36481 atcggcatcc agttcaggta aacgctccgc gctcacatcc attcgcccac cttcaggaat
    36541 gctctcaatc agcggcggaa agcgaaaacc agcatcgcgt aatacccgcc ccagcgaatg
    36601 atagctatgc atcacattaa tttttccctg atttgcctga atcaccgata ccgttatttt
    36661 ttggctgtcc agcgtcgcct tcagcgcatt gatttgtgcc tgatagcggc gttcaagtat
    36721 cgccagctgc gactgcgttc ctgtcagttc tgccagcttg cgatagattt ctggcgcgcc
    36781 ccctttgaga tgatcaatgc tcaccgtggg cgcaattttt tccagtcgtt caatgggtgt
    36841 attacgggtc ggttctgtga tgatcaggtc aggctttgcc gccacaatcg cttcaatatc
    36901 aatatcagcg gtgccaataa aagcgatcga tgaattatca aagtcgacac ctgtcaataa
    36961 cgcgcctgat cgaatgaaat ggctaccgtc aggccgagtt cgcccgtggc tggcgacagg
    37021 aggtacaccg agctcaatca gaggaatagt gatatcaaga tcgtgtaaag agacaattct
    37081 ctttggatga gcaggaacag tgactttgcg ccctaaatca tcagtaaaga cctgaacagg
    37141 ttctgtcgcg cgggcaaaga atgacaacag tatcagcaga gaaaaaaata aacgcatata
    37201 actcccatta aaaactgttc ctgcgctgcc agagcagtag caaaaagaat ggtccaccaa
    37261 tcaatgaaat aatgattccg gcaggcagtt gcaaaggcaa aaaagccaaa cgaccaatgc
    37321 tatccgccag gatgactaac aacgcccccg atacggcgct ccccagcagt aatgtcgtct
    37381 gcccgccacg tagaataaaa cgtgatatat ggggtgctat caatccgaca aaaccgatgt
    37441 taccaacaca cgaaacgcag acggctgtca ggattattgg tgcggcgaca cgtaacaacg
    37501 ccagacgaga actattcacc cctaacccgg ttgcaacctg atgaccaagc agagcgatat
    37561 ccgccgtcct ggcggtgaac agcaataaaa gagcggctgg caacatccaa caggctgaaa
    37621 taccgactaa catccagtta gcagcatgca ggcttcccga taaccacatc aatgcagttt
    37681 gtacatctcg aacatctgca gtggtcataa atacaccgat accggcggcg aaaaaccagg
    37741 aaacaccaat accaatcaga acaaatcgcg ggcgggaaat ttcgcgtgca caaaaaatca
    37801 cgattaaagc caccaacaag ccaccagcaa gaccagccac tggacgccag aacattccca
    37861 gcattggaaa ctggaaaatc agccacagca ccgccacgct acatccttct ttgacgccga
    37921 ttaaaccagg atcggcaagg ccgttacgcg caatggattg catcgcggcc cccgccatac
    37981 ctaacatggc tccgcatagc aatgccatga tgactctcgg caagcgaata tcctgcacaa
    38041 tataacgtgc gtctgtgctt acgttttctg gagcaaacaa cgctcgtcct acttccgatg
    38101 ccgggacaaa aaaagaacca tgcatcagac cgaatatcag tattccaacc gcaaacagaa
    38161 acagcccacc aagcaacacc agatttttag ggcgtaccag tgtcgaaaac ttaccaacac
    38221 gtagtggacg tagcccgacg ttcattatcc gactcatttg aacattctcg tcgccataat
    38281 gacaaaaaca ggcgcaccaa ctaacgccgt catgacaccg gtcgctaact catggggcgt
    38341 gaaaagcgtc cgagcaataa tatctgccag taataacaaa agcgcgccga cacaagctga
    38401 aagagggagc agaacgcgca aatccgctga taccagacgc ctgacaatct gcggtactag
    38461 cagaccgaca aaaccaatgg ggccagcaat cgatacggct gcgccacaga gcaacgcgat
    38521 cgcaagcaga gtgaatgttc gcgtgcggag taccgagacg cccagcccct gcgccatgcg
    38581 atcacccagt gccaacatgt taagagaggg cgcaaggtaa atggctaaca caaccccccc
    38641 cagagaaaac caggcggacg tacccagcgt cgcccaatcc tgccccgcta aatcacctgc
    38701 tagccatgtc cgcatcgcca gcagtgtttg ttcgtcaaga atcaggattg cagccgttat
    38761 tgaggacacg aacgccgata gcgccacgcc gcataaagta actttcattg gcgttagccc
    38821 tgaacgccct gcggaggaaa gcagcaatat caacaggaac aataacgcgc cgcccgtaga
    38881 tgccagcagt gggcgtccaa ccggaaaggc cagcccaagc gcactggcag caacgacagc
    38941 aagagcggct ccggcattta agcctaaaat atgtggctcc cccaatgggt tacgaatgac
    39001 agcctgcaac aaagcaccag caactccaag agaagcaccg gtaagcaatg ctgccgctaa
    39061 acgcggcaaa cggagtctta caattacgtt gtgatcgaag ttttgcggtt caaaatgaag
    39121 aaatgcctgc aacaccgttt gtggtgcaat aaatcgggca ccaatccctg gatataccac
    39181 gcatcccaca agcagtaagg cgaggaaaat tacagtaacc agccctgtgc gcatcatact
    39241 ttttctacac cacgaaacgg catgaagaac ggctttcccg tcagagtatt tattgatgcg
    39301 tgaacatcaa cgtcaaagac agcttttacc agctcaggcg tgcaatgctc cccctcattc
    39361 aacacacgca ccactttccc ctggcgtaaa aatatcaacg tatcaccata attgacggca
    39421 aaattcagat catgcaacac cacgacgact gtacgcccgt gatgacgcgt taaatcatgt
    39481 aataactcaa gaatttcaac ctgataacgt aaatcaagcc aggttgtggg ttcatccagt
    39541 aggatataag gcgtcttttg cgccaacacc atcgcaatcc agcaacgctg gcgctgccca
    39601 ccagagagtt tttcaacagg taaatgagca aactcctgcg taccggttaa tttcagggcc
    39661 tcttctaccg cctgctcgtc ggcgtcgctc cattgacgta tgaaattttg ccagggaaaa
    39721 cgcccacgcg acaccagttc ataaacggtt aagccctccg gcaataatgg cgactgcggc
    39781 aagatcccaa gtctacggga taacgctttc gttggttgct cgtgaatcgc tttaccatcc
    39841 agcaatattg agccgcccat gggttgcaag acacgtgcaa tggtgctgag caacgtcgac
    39901 tttccacagc cgtttgcccc aaccagaacg gtcatttttt ctgtgggaac ggagaaagaa
    39961 acaccatcaa caatgatttt cttgtgatac ccggcagaaa cattgtcgag gatcagcccc
    40021 tgcccttttg cgttatgtgc cacgtgtacg ccaacttaat caaagaaaaa caaaaataaa
    40081 actcattctc ttttgagagt ttgacgccac aaaatgtagt agtcaagaa
//
LOCUS       DAZFBR010000031        39350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFBR010000031 DAZFBR010000000
VERSION     DAZFBR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.031"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            393..1652
                     /gene="codB"
                     /locus_tag="ACHJZ2_001864"
     CDS             393..1652
                     /gene="codB"
                     /locus_tag="ACHJZ2_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO4567988.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1642..2925
                     /gene="codA"
                     /locus_tag="ACHJZ2_001865"
     CDS             1642..2925
                     /gene="codA"
                     /locus_tag="ACHJZ2_001865"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO4567989.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(3058..3957)
                     /gene="cynR"
                     /locus_tag="ACHJZ2_001866"
     CDS             complement(3058..3957)
                     /gene="cynR"
                     /locus_tag="ACHJZ2_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO4567990.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            4067..4726
                     /gene="cynT"
                     /locus_tag="ACHJZ2_001867"
     CDS             4067..4726
                     /gene="cynT"
                     /locus_tag="ACHJZ2_001867"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO4567991.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4757..5227
                     /gene="cynS"
                     /locus_tag="ACHJZ2_001868"
     CDS             4757..5227
                     /gene="cynS"
                     /locus_tag="ACHJZ2_001868"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO4567992.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            5233..6414
                     /gene="cynX"
                     /locus_tag="ACHJZ2_001869"
     CDS             5233..6414
                     /gene="cynX"
                     /locus_tag="ACHJZ2_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO4567993.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(6517..7128)
                     /gene="lacA"
                     /locus_tag="ACHJZ2_001870"
     CDS             complement(6517..7128)
                     /gene="lacA"
                     /locus_tag="ACHJZ2_001870"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO4567994.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(7194..8447)
                     /gene="lacY"
                     /locus_tag="ACHJZ2_001871"
     CDS             complement(7194..8447)
                     /gene="lacY"
                     /locus_tag="ACHJZ2_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO4567995.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(8499..11573)
                     /gene="lacZ"
                     /locus_tag="ACHJZ2_001872"
     CDS             complement(8499..11573)
                     /gene="lacZ"
                     /locus_tag="ACHJZ2_001872"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO4567996.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(11696..12778)
                     /gene="lacI"
                     /locus_tag="ACHJZ2_001873"
     CDS             complement(11696..12778)
                     /gene="lacI"
                     /locus_tag="ACHJZ2_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO4567997.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(12815..13768)
                     /locus_tag="ACHJZ2_001874"
     CDS             complement(12815..13768)
                     /locus_tag="ACHJZ2_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFO4567998.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(13788..14564)
                     /locus_tag="ACHJZ2_001875"
     CDS             complement(13788..14564)
                     /locus_tag="ACHJZ2_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO4567999.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(14679..15512)
                     /gene="mhpR"
                     /locus_tag="ACHJZ2_001876"
     CDS             complement(14679..15512)
                     /gene="mhpR"
                     /locus_tag="ACHJZ2_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO4568000.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15703..17367
                     /gene="mhpA"
                     /locus_tag="ACHJZ2_001877"
     CDS             15703..17367
                     /gene="mhpA"
                     /locus_tag="ACHJZ2_001877"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO4568001.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            17369..18313
                     /gene="mhpB"
                     /locus_tag="ACHJZ2_001878"
     CDS             17369..18313
                     /gene="mhpB"
                     /locus_tag="ACHJZ2_001878"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO4568002.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            18331..19197
                     /gene="mhpC"
                     /locus_tag="ACHJZ2_001879"
     CDS             18331..19197
                     /gene="mhpC"
                     /locus_tag="ACHJZ2_001879"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO4568003.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            19207..20016
                     /gene="mhpD"
                     /locus_tag="ACHJZ2_001880"
     CDS             19207..20016
                     /gene="mhpD"
                     /locus_tag="ACHJZ2_001880"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO4568004.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            20013..20963
                     /gene="mhpF"
                     /locus_tag="ACHJZ2_001881"
     CDS             20013..20963
                     /gene="mhpF"
                     /locus_tag="ACHJZ2_001881"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO4568005.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            20960..21973
                     /gene="mhpE"
                     /locus_tag="ACHJZ2_001882"
     CDS             20960..21973
                     /gene="mhpE"
                     /locus_tag="ACHJZ2_001882"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO4568006.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            22149..23360
                     /gene="mhpT"
                     /locus_tag="ACHJZ2_001883"
     CDS             22149..23360
                     /gene="mhpT"
                     /locus_tag="ACHJZ2_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO4568007.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            23462..24001
                     /gene="yaiL"
                     /locus_tag="ACHJZ2_001884"
     CDS             23462..24001
                     /gene="yaiL"
                     /locus_tag="ACHJZ2_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO4568008.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(24126..24959)
                     /gene="fghA"
                     /locus_tag="ACHJZ2_001885"
     CDS             complement(24126..24959)
                     /gene="fghA"
                     /locus_tag="ACHJZ2_001885"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4568009.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(25052..26161)
                     /gene="frmA"
                     /locus_tag="ACHJZ2_001886"
     CDS             complement(25052..26161)
                     /gene="frmA"
                     /locus_tag="ACHJZ2_001886"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO4568010.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(26196..26471)
                     /gene="frmR"
                     /locus_tag="ACHJZ2_001887"
     CDS             complement(26196..26471)
                     /gene="frmR"
                     /locus_tag="ACHJZ2_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO4568011.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(26631..27677)
                     /gene="fbpC"
                     /locus_tag="ACHJZ2_001888"
     CDS             complement(26631..27677)
                     /gene="fbpC"
                     /locus_tag="ACHJZ2_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO4568012.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(27689..29767)
                     /locus_tag="ACHJZ2_001889"
     CDS             complement(27689..29767)
                     /locus_tag="ACHJZ2_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4568013.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(29836..30867)
                     /locus_tag="ACHJZ2_001890"
     CDS             complement(29836..30867)
                     /locus_tag="ACHJZ2_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4568014.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(30864..32180)
                     /gene="uhpC"
                     /locus_tag="ACHJZ2_001891"
     CDS             complement(30864..32180)
                     /gene="uhpC"
                     /locus_tag="ACHJZ2_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO4568015.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(32253..33794)
                     /locus_tag="ACHJZ2_001892"
     CDS             complement(32253..33794)
                     /locus_tag="ACHJZ2_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO4568016.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(33794..34423)
                     /locus_tag="ACHJZ2_001893"
     CDS             complement(33794..34423)
                     /locus_tag="ACHJZ2_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO4568017.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            34726..35688
                     /gene="tauA"
                     /locus_tag="ACHJZ2_001894"
     CDS             34726..35688
                     /gene="tauA"
                     /locus_tag="ACHJZ2_001894"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4568018.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            35701..36468
                     /gene="tauB"
                     /locus_tag="ACHJZ2_001895"
     CDS             35701..36468
                     /gene="tauB"
                     /locus_tag="ACHJZ2_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO4568019.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            36465..37292
                     /gene="tauC"
                     /locus_tag="ACHJZ2_001896"
     CDS             36465..37292
                     /gene="tauC"
                     /locus_tag="ACHJZ2_001896"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO4568020.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            37289..38140
                     /gene="tauD"
                     /locus_tag="ACHJZ2_001897"
     CDS             37289..38140
                     /gene="tauD"
                     /locus_tag="ACHJZ2_001897"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO4568021.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRSLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(38247..39221)
                     /gene="hemB"
                     /locus_tag="ACHJZ2_001898"
     CDS             complement(38247..39221)
                     /gene="hemB"
                     /locus_tag="ACHJZ2_001898"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO4568022.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         9055 a        10382 c        10810 g         9103 t
ORIGIN      
        1 cgacaccgtg ctcagtgcct gatgcgacgc tgatgcgtct tatcatgcct acaggcctga
       61 accgtaggtc ggataaggcg ctcgcgccgc atccgacacc gtgcgcagat gcctgatacg
      121 acgcaggcgc gtcttatcat gcctacaggc ctgaaccgta ggtcggataa ggcgctcgcg
      181 ccgcatccga caccgtgcgt agttgcctga tgcgacgctg acgcgtctta tcatgcctac
      241 ccactgtttt tacaccgata attttccccc cacctttttg cactcattca tataaaaaat
      301 atatttcccc acgaaaacga ttgcttttta tcttcagatg aatagaatgc ggcggatttt
      361 ttgggtttca aacagcaaaa agggggaatt tcgtgtcgca agataacaac tttagccagg
      421 ggccggtccc gcagtcggcg cggaaagggg tattggcatt gacgttcgtc atgctgggat
      481 taaccttctt ttccgccagt atgtggaccg gcggcactct cggaaccggt cttagctatc
      541 atgatttctt cctcgcagtt ctcatcggta atcttctcct cggtatttac acttcatttc
      601 tcggttacat tggcgcaaaa accggcctga ccactcatct tcttgctcgc ttctcgtttg
      661 gtgttaaagg ctcatggctg ccttcactgc tgctgggcgg cacccaggtt ggctggtttg
      721 gcgtcggtgt ggcgatgttt gctattccgg tgggcaaggc aaccgggctg gatattaatt
      781 tgctgattgc cgtttccggt ttactgatga ccgtcaccgt cttctttggc atttcggcgc
      841 tgacggttct ttcgttgatt gcggttccgg ctatcgcctg tcttggcggt tattccgtgt
      901 ggctggccgt taacggcatg ggcggcctgg acgccttaaa agcggtcgtt cccgcacaac
      961 cgttagattt caatgtcgcg ctggcgctgg ttgtggggtc atttatcagt gcgggcacgc
     1021 tcaccgctga ctttgtccgc tttggtcgca atgccaaact ggcggtgctg gtggcgatgg
     1081 tggccttttt cctcggcaac tcgttgatgt ttattttcgg tgcagcgggc gctgcggcac
     1141 tggggatggc ggatatctct gatgtgatga ttgctcaggg cctgctgttg cctgcgattg
     1201 tggtgctggg gctaaatatc tggaccacca acgataacgc gctctatgcg tcgggtttag
     1261 gtttcgccaa cattaccggg atgtcgagca aaaccctttc ggtaatcaac ggtattatcg
     1321 gtacggtctg tgcattatgg ctgtataaca attttgtcgg ctggttgacc ttcctttcgg
     1381 cagctattcc tccagtgggt ggcgtgatca ttgccgacta tctgatgaac cgtcgccgct
     1441 atgagcactt tgcgaccacg cgtatgatga gtgtcaattg ggtggcgatt ctggcggtcg
     1501 ccctggggat tgctgcaggc cactggttac cgggaattgt tccggtcaac gcggtattag
     1561 gtggcgcgct gagctatctg atccttaacc cgattttgaa tcgtaaaacg acagcagcaa
     1621 tgacgcatgt ggaggctaac agtgtcgaat aacgctttac aaacaattat taacgcccgg
     1681 ttgccaggca aagaggggct gtggcagatt catctgcagg acggaaaaat cagcgccatt
     1741 gatgcgcaat ccggcgtgat gcccataact gaaaacagcc tggatgccga acaaggttta
     1801 gttataccgc cgtttgtgga gccacatatt cacctggaca ccacgcaaac cgccggacaa
     1861 ccgaactgga atcagtccgg cacgctgttt gaaggcattg aacgctgggc cgagcgcaaa
     1921 gcgttattaa cccatgacga tgtgaaacaa cgcgcatggc aaacgctgaa atggcagatt
     1981 gccaacggca ttcagcatgt gcgtacccat gtcgatgttt cggatgcaac gctaactgcg
     2041 ctgaaagcaa tgctggaagt gaagctggaa gtcgcgccgt ggattgatct gcaaatcgtc
     2101 gccttccctc aggaagggat tttgtcgtat cccaacggtg aagcgttgct ggaagaggcg
     2161 ttacgcttag gggcagatgt agtgggggcg attccgcatt ttgaatttac ccgtgaatac
     2221 ggcgtggagt cgctgcataa aaccttcgcc ctggcgcaaa aatacgaccg tctcatcgac
     2281 gttcactgtg atgagatcga tgacgagcag tcgcgctttg tcgaaaccgt tgctgccctg
     2341 gcgcaccgtg aaggcatggg cgcgcgagtc accgccagcc acaccacggc aatgcactct
     2401 tataacgggg cgtatacctc acgtctgttc cgcttgctga aaatgtccgg tattaacttt
     2461 gtcgccaacc cgctggtcaa tattcatctg caaggacgat tcgatacgta tccaaaacgt
     2521 cgcggcatca cgcgcgttaa agagatgctg gagtccggca ttaacgtctg ctttggtcac
     2581 gatgatgtct tcgatccgtg gtatccgctg ggaacggcga atatgctgca agtgctgcat
     2641 atggggctgc atgtttgcca gctgatgggc tatgggcaga ttaacgatgg cctgaattta
     2701 atcacccacc acagcgccag gacgttgaat ttgcaggatt acagcattgc cgccggaaac
     2761 agcgccaacc tgattatcct gccggctgaa aatggatttg atgcgctgcg ccgtcaggtt
     2821 ccggtacgtt attcggtacg tggcggcaag gtgattgcca gcacacaacc ggcacaaacc
     2881 accgtatatc tggagcagcc ggaagccatc gattacaaac ggtgatcggc tgggctgtgt
     2941 gttgacaaag cgagcgtagt ttatgccgga tgcggcttga acgccttatc aggcctacgt
     3001 agagcactga actcgtaggc ctgataagcg taacgcatca ggcaattgtg tcagtggtta
     3061 ccgcgattca ttctcgccaa catccgcaca ttcctccaac gccatgtgca aaaatgcctt
     3121 cgcggcggct gtctgccagc tatttttccg ccgcaacaaa accgccgttc tctccagtag
     3181 tggcggggca agaggaatcg ctttaagccc gtcatgttgt gtggcaatcg ctgctggtaa
     3241 caatgtggaa agggaagtgc ggcgaatcag ctccagaacc gcgctaattg agttcgcctc
     3301 aatgaccacc tgtggatgca gccccgcttt ctcgcagtag tggtcaatct gctctctggt
     3361 ggcaaattcc gcgctgagca ggaccagttt ttcatcatgc aagcgactca acgccacctg
     3421 ttcacaggca gccagcggat gatgttgcgc cacgactaac gctaaacttt ctgtcagtaa
     3481 aggaattgcc tccagctccg gcaaatgcac aggcgcgaag gcaatcccaa cgtccagctc
     3541 gtcgcgacaa agcagatctt cgattttctc ctgcgacatt tcctgtagct ggagcgtgat
     3601 gccggggtag cgcgcataga aatccgccat taaggggccg ataaagtagc tcgtaaaggt
     3661 gggggtgacg gcgatacgca gcgatcctct cgtcagatcg gcaacatcat gaatcgcccg
     3721 tttacccgct cccagttcct gtaacgcccg gctggcgtac tgtcgccaga cttctcctgc
     3781 atcagtgaga tgaatcgttc gcccgctacg gtcaaacagc ggcacgccta aactctcctc
     3841 taactggcga atctgctggg aaagcgcagg ctgagagacg tgcaacgcac tggcggcacg
     3901 ggtgaagctg ccatgttcag ccacggcaag aaaataattg atatgtcgag agagcattcg
     3961 ccaacctata agtaaattca atagaactca tcataaatga gacttttacc ttatgacaat
     4021 cggcgagtag tctgcctctc attccagaga cagatagagg ttaacggtga aagagattat
     4081 tgatggattc cttaaattcc agcgcgaggc atttccgaag cgggaagcct tatttaaaca
     4141 gttggcgaca cagcaaagcc cgcgcacact ttttatctcc tgctccgaca gccgtctggt
     4201 ccctgagctg gtgacgcaac gtgagcctgg cgatcttttc gttattcgca atgcgggcaa
     4261 tatcgtccct tcctacgggc cggaacccgg tggcgtttct gcttcggtgg agtatgccgt
     4321 cgctgcgctt cgggtatctg acattgtgat ttgcggtcat tccaactgtg gcgcgatgac
     4381 cgccattgcc agctgtcagt gcatggacca tatgcctgcc gtctcccact ggctgcgtta
     4441 tgccgattca gcccgcgtcg ttaatgaggc gcgcccgcat tccgatttac cgtcaaaagc
     4501 tgcggcgatg gtacgtgaaa acgtcattgc tcagttggct aatttgcaaa ctcatccatc
     4561 ggtgcgcctg gcgctcgaag aggggcggat cgccctgcac ggctgggtct acgacattga
     4621 aagcggcagc atcgcagcgt ttgacggcgc aacccgccag tttgtgccac tggcagctaa
     4681 tcctcgcgtt tgtgccatac cgctacgcca accgaccgca gcgtaacctt atttttaaac
     4741 catcaggaga tgcaccatga ttcagtcgca aattaaccgc aatattcgtc ttgatcttgc
     4801 cgatgccatt ttgctcagca aagctaaaaa agatctctca tttgccgaga ttgccgacgg
     4861 caccgggctg gcagaagcct ttgtgaccgc ggctttgctg ggtcagcagg cgcttcctgc
     4921 cgacgccgcc cgccaggtcg gggcgaagct ggatctcgac gaagacgcca ttctactgtt
     4981 acagatgatc ccactgcgtg gctgcattga tgaccgtatt ccaaccgacc caacgatgta
     5041 tcgtttctat gaaatgttgc aggtgtacgg cacaaccctg aaagcgttgg ttcatgagaa
     5101 atttggcgat ggcattatca gcgcgattaa cttcaaactc gacgttaaga aagtggcgga
     5161 cccggaaggt ggcgaacgtg cggtcatcac cttagatggt aaatatctgc cgaccaaacc
     5221 gttctgacag ccatgcgtaa ccatcaaaag acgttcacga tgctgctggt tctggtgctg
     5281 attggtctta atatgcgacc actgctcacc tccgtcgggc cactgctacc gcaattgcgc
     5341 caggcaagcg gaatgagctt tagcgtggct gccctgttga ccgctctgcc ggtggttacc
     5401 atgggcgggc tggcgctggc cggaagctgg cttcatcagc atgtcagcga acgtcgcagc
     5461 gtcgccatca gtttgttact gattgccgtc ggtgcattga tgcgtgagct ttaccctcaa
     5521 agtgcgctgc tgcttagcag cgcactgctc ggtggggtgg ggatcggcat cattcaggcg
     5581 gtgatgcctt cggtgattaa acggcggttt cagcagcgca cgccactggt gatggggctg
     5641 tggtccgcgg ctctgatggg cggcggtggg cttggtgccg ccctgacacc ctggttagtt
     5701 caacatagcg aaacctggta tcaaacactc gcctggtggg cgctgcctgc cgttgttgcg
     5761 ctctttgcct ggtggtggca aagcacccgc gaggtcgcct cttcccacaa gacaacaacc
     5821 attccggttc gcgtggtttt cactcctcgc gcgtggacgc tgggtgttta cttcggtctg
     5881 attaacggtg gttacgccag cctgattgcc tggttacccg ccttctatat tgagattggt
     5941 gccagcgcgc agtacagcgg ttccttgctg gcattgatga cgcttgggca agccgcagga
     6001 gctttgctga tgcctgctat ggctcgccat caggatcggc gcaaactgtt aatgctggcg
     6061 ctggcgttac aactggtggg tttctgcggc tttatctggc tgccgctgca attgccggta
     6121 ttgtgggcga tggtgtgtgg tttaggtctg ggcggcgcat ttccgctctg tttgctgctg
     6181 gcgctcgatc actctgtgca accggctatt gctggcaagc tggtggcgtt tatgcaggga
     6241 atcggtttta tcatcgccgg gcttgccccg tggttttctg gcgtgctgcg tagtatcagc
     6301 ggcaattact tgatggactg ggcgtttcat gcgctgtgcg tcgtagggct gatgattata
     6361 accctgcgtt ttgcaccagc acgttttccg cagctgtggg tcaaagaggc atgatgcgac
     6421 gcttgtttct gcgctttgtt cacgccggat gcggcctaca tagatcactg aatttgtagg
     6481 cctgataagc gaagcgtatc aggcaatttt tataatttaa actgacgatt caactttgta
     6541 atctctgaaa taatattgct tatcccggtc gtttatttcg cgaataaccc gacaaggaac
     6601 gccagccgcc acgacgtttg gtggaatgtc ttttgtgacg atactacccg cgccaataac
     6661 agaattatcc ccaatggtga cgcctggatt aataaccaca tgacttccga tccagacgtt
     6721 attgccaatc gttatcggaa aagagtacat ctcaccgttt tttctcaatt catggtgtac
     6781 agggtgtccc gtaacggaaa gagtaacgtt gggtgcaatc aatacgttat caccgattgt
     6841 taccgtgtag tcatcgacaa tggttaaatt gaaatttgca taaaaattgc ggcctatatg
     6901 gatgttggaa ccgtaagaga aatagacggg gggttctacc caggcgtttt cccctaccgt
     6961 ggcaaacatt tctttaatca ggctttctct tttatcaact tctgatggat gcgagtgatt
     7021 aaactcatac attaacgttt ttctacgaag tcttttttca ggtaagcctt cgcacatatc
     7081 ggtaaatagc ttgcctgctt ttattctttc tgtcatcgac atgttcattg cgatcattcc
     7141 gttatgatat gttggtcgga taaggcgctc gcgccgcatc cgacattgat tgcttaagcg
     7201 acttcattca cctgacgacg cagtagagaa agcgggccgg ggccgctaag cgtgaacacg
     7261 gaaattaagg tgaagcccag cgccaccaga cccagcacca gataagcgcc ctggaaaccg
     7321 atgctttcat acatattacc cgctagtaca gacataaaaa tcatcgccag ttgcttaaag
     7381 aagcagaaac agaccagata aatcgtcgct gaaaaacgca cttcaaactg gctggtaata
     7441 tatttaaagc agcccaccag caggaacggt acttcaaaca tatgcagcgt tttcagaata
     7501 accacttcca gcgctgaggt ggcgaacgat gagccaataa tacgtacaga cataatagtg
     7561 ccagccagca gcagggcatt tttcccaccg atgcgattaa tgatcagtgg cgcaaagaac
     7621 ataattgagg cgttaagtaa ttcgcccatt gtcgttacgt agccaaatac ccgcgtaccc
     7681 tgttcaccgg tggcaaagaa agaagtaaag aaattagcaa actgttggtc aaaaacatcg
     7741 taggtgcagg aaacgccaat aacatacagt gacaaaaacc acagttttgg ctgtctgaac
     7801 agttccagcg ccagtttaag gctaaatgcc gaatggttgg cacctaccgc attggcaacc
     7861 gtggcggaag agggcgcatc cgttttggcg aaaaagagta aaatggcgag gatgagtgca
     7921 cagccagaac ccagccagaa aacgaactga ttattgatgg tgaacatgat gccgacaatc
     7981 gaggcacaca gcgcccagcc aacacagcca aacatccgcg cgcgaccaaa ttcgaaatta
     8041 ctgcgacggc tgactttctc gataaatgcc tctactgcgg gcgcaccggc gttaaaacaa
     8101 aagccaagat aaataccacc aacaatcgat cctactaaaa tgttgtattg taacagtggc
     8161 ccgaagataa aaataaagaa cggcgcaaac atcactaaca tgccggtaat aatccacagc
     8221 aggtatttgc gcagcccgag tttgtcagaa agcagaccaa acagcggttg gaataatagc
     8281 gagaacagag aaatagcagc aaaaataata cccgtatcac ttttgctgat atggttgatg
     8341 tcatgtagcc aaatcgggaa aaacgggaag taggctccca tgataaaaaa gtaaaagaaa
     8401 aagaataaac cgaacatcca aaagtttgtg ttttttaaat agtacataat ggatttcctt
     8461 acgcgaaata cgggcagaca tggcctgccc ggttattatt atttttgaca ccagaccaac
     8521 tggtaatggt agcgaccggc gctcagctgg aattccgccg atactgacgg gctccaggag
     8581 tcgtcgccac caatccccat atggaaaccg tcgatattca gccatgttcc ttcttccgcg
     8641 tgcagcagat ggcgatggct ggtttccatc agttgttgtt ggctgtagcg gctgatgttg
     8701 aactggaagt cgccgcgcca ctggtgtggg ccataattca attcgcgcgt cccgcagcgc
     8761 agaccgtttt cgctcgggaa gacgtacggg gtatacatgt ctgacaatgg cagatcccag
     8821 cggtcaaaac aggcggcagt aaggcggtcg ggatagtttt cttgcggccc taatccgagc
     8881 cagtttaccc gctctgctac ctgcgccagc tggcaggtca ggccaatccg tgccggatgc
     8941 ggtgtatcgc tcgctacttc aacatcaacg gtaatcgcca tttgaccact accatcaatc
     9001 cggtaggttt tccggctaat aaataaggtt tttccctgat gctgccatgc gtggacagtg
     9061 gtaatcagca ccgcgtcggc aagtgtatct gccgtgcact gcaacaacgc cgcttctgcc
     9121 tggtaatggc ccgcagcctt ccagcgttcg acccaggcgt tagggtcaat gcgggtcgct
     9181 tcacttacgc caatgtcgtt atccagcggt gcgcgggtga actgatcgcg cagcggcgtc
     9241 agcagttgtt ttttatcgcc aatccacatc tgtgaaagaa agcctgactg gcggttaaat
     9301 tgccaacgct tattatccag ctcgatgcaa aaatccgttt cgctggtggt cagttgcggg
     9361 atggcgtggg gcgcggaggg gagtgtcacg ctgaggtttt ccgccagacg ccactgctgc
     9421 caggcgctga tgtgtccggc ttctgaccat gcggtcgcgt tcggttgcac tacgtgtacc
     9481 gttagccaga gttgcccggt gctctccagt cgcggtagtt caggcaattc aattacctgt
     9541 ttaccttgcg gagcgacatc cagaggcact tcaccgcttg ccagcggctt accatccagc
     9601 gccaccgtcc agtgcaggag ctcgttatcg ctatgacgga acaggtattc gctggtcact
     9661 tcgatggttc gcccggataa acggaactgg aaaaactgct gctggtgttt tgcttccgtc
     9721 agcgccggat gcggcgtgcg gtcggcaaag accagaccgt tcatgcagaa ctggcgatca
     9781 ttcggcgtat cgccaaaatc accgccgtaa gccgaccacg gattgccatt ttcatcatat
     9841 ttaatcagcg actgatccac caagtcccag acgaagccgc cctgtaaacg ggggtactga
     9901 cgaaacgcct gccagtattt agcgaagccg ccaagactgt tacccatcgc gtgggcgtat
     9961 tcgcaaagga tcagtgggcg catttctcca ggcagcgaaa gccatttttt gatggaccat
    10021 ttcggcacag ccgggaaggg ctggtcttca tccacgcgcg cgtacatcgg gcaaataata
    10081 tcggttgcgg aggtgtcggc tccgccgcct tcatactgca ccgggcggga tggatcgaca
    10141 gatttaatcc agcgatagag tgcgtcgtga ttagcgccgt ggcctgactc attccccagc
    10201 gaccagatga tcacactcgg gtgattacga tcgcgctgca ccattcgtgt tactcgttcg
    10261 ctcatggccg gtaaccagcg cggatcatcg gtcagacgat tcattggcac catgccgtgg
    10321 gtttcaatat tggcttcatc caccacatac aggccgtagc ggtcgcacag ggtgtaccac
    10381 agcggatggt tcggataatg cgaacagcgc acggcgttaa agttgttctg cttcattagc
    10441 aggatatcct gcaccatcgt ctgctcatcc atgacctgac catgcagagg atgatgctcg
    10501 tgacggttaa cgccgcgaat cagcaacggc ttgccgttca gcagcagcag accattttca
    10561 atccgcacct cgcggaaacc gacatcgcag gcttctgctt caatcagcgt gccgtcggcg
    10621 gtgtgcagtt caaccaccgc acgatagata ttcgggattt cggcgctcca cagtttcggg
    10681 ttttcgacgt tcagacctag tgtgacgcga tcggcataac cgccacgctc atcgataatt
    10741 tcaccgccga aaggcgcggt gccgctggcg acctgcgttt caccctgcca caaagaaacc
    10801 gtcacccgca gctcatcgcg cagctcgccg tacatctgaa cttctgcctc cagtaccgcg
    10861 cggctaaaat catcattaaa gagagtggca acatggaaat cgctgatttg tgtggtcggt
    10921 ttgtgcagca acgagacgtc acggaaaatg ccgctcatcc gccacatatc ctgatcttcc
    10981 agataactgc cgtcactcca gcgcagcacc atcaccgcga ggcggttttc tccggcgcgt
    11041 aaaaatgcgc tcaggtcaaa ttcagacagc aaacgactgt cctggccgta accgacccag
    11101 cgcccgttgc accacagatg aaacgccgag ttaacgccat caaaaataat tcgcgtctgg
    11161 ccttcctgta gccagctttc atcaacatta aatgtgagcg agtaacaacc cgtcggattc
    11221 tccgtgggaa caaatggcgg attgaccgta atgggatatg tcacgttggt gtagatgggc
    11281 gcgtcgtaac cgtgcatctg ccagtttgag gggacgacga cagtatcggc gtcaggaaga
    11341 tcgcactcca gccagctttc cggaaccgct tctggtgccg gaaaccagac aaattgccac
    11401 tcaccattca ggctgcgcaa ctgctgggaa gggcgattgg tgcgggcctc ttcgctatta
    11461 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    11521 tcccagtcac gacgttgtaa tacgacggcc agtgaatctg taatcatagt catagctgta
    11581 tcctgtgtga aattgttatc cgctcacaat ttcacacaac atacgagccg gacgcataga
    11641 gtgtaaagcg tggggtgcct aatgagtgag ctgactcaca ttaattgcgt tgcgctcact
    11701 gcccgctttc cagtcgggaa acttgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    11761 ggggagaagc ggtttgcgtc ttgggcgcca gggtggtttt tcttttcacc agtgagacgg
    11821 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
    11881 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacacg
    11941 agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcaacccgg
    12001 actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag
    12061 tgggaacgat gccctcattt agcatttgca tggtttgttg aaaaccggac atggcactcc
    12121 agtcgccctc ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc
    12181 ccgccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct
    12241 ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa
    12301 taatactgtt gatgggagtc tggtcagaga catcaagaaa taacgccgga acattagcgc
    12361 aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac
    12421 tgacgcgttg cgcgagaaga ttgtgcactg ccgctttaca ggcttcgacg ccgcttcgtt
    12481 ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga
    12541 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
    12601 gtttccccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg
    12661 cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctgattcacc acgcgggaaa
    12721 cggtctgata agagacaccg gcatactctg cgacatcgta tagcgttact ggtttcacat
    12781 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
    12841 gccattcggc ggggctgacg ccgaagtgag atttaaaatg ctgacgccag gtcacaggtg
    12901 agagaaaacc cacctcagcc gctatccgct caacgggcaa ctgactggat cccaacagtt
    12961 cctggctacg gcgtaagcgt tcagtaatga gccattcggc gatactcgaa ccggtcgctt
    13021 tcataaaatg gcgagttaat gtgcggcgac tcatcattac tcgctgcgcc agcgagtcga
    13081 gatcatgctg ttgcgcaatg ttttgccgca ggtaatccag caggaggttt atgcgttcat
    13141 catgggtatt tttcggcacc ggttgctcaa taaactgcgc ctgaccgccg gtgcgatatg
    13201 gcggtacgac catccgtcgg gcaatatggt tagcatagtc gctgccaaaa tgttgccgaa
    13261 caatatacaa acagcaatcc agcgccgcgg cagtaccggc agaggtaata atgccgtcat
    13321 cgtctacgta caacgcgtta atatccagat gtgtttgtgg aaaacgtgcc tgaaattcac
    13381 gctcgaactc ccagtgcgtg gcggcacgct tatttttcag taaacctgca tagccgagca
    13441 caaacgtgcc caggcaaagc ccgacaatct gtgcgccgtt atcccgtgcg gtcgttaagg
    13501 cttccagcag tttttgtggc ggtttttgtg taatggttcc ccagtaagga attatcacaa
    13561 tatctgcctg gatgactgcg gtatagtcat gggtggcggt tacggaaaac ccatcggcag
    13621 agtccacgtt tcccggcttt tcagcgcaaa taatcacgtg aaagcgtttt ttctccgcca
    13681 tcttttcact aaacacgata aacggtacgg aaaggtgaaa aggactaaaa ccggcaacgg
    13741 cgataatggc aacagttagc gggcgcataa cactctttat ctccgaatta gaatgtcaac
    13801 gtttcaccat cttctggtac gttcacaaat tcgataattt gattatctct ggcgtattcc
    13861 ttgagcgcat cacgcgtcag caaacagtgg tttacggcct ccatatgtgt cgcgataatt
    13921 tgcgcttctg ggagggtaaa atgtgttttg agaatatctt ctgctcccat aattatcgcg
    13981 ccatgcccca gaacatgcgc aaatccggca ttcaacacaa ccacttctgg ttggaaagtt
    14041 agcattgtag cctcgacgtc gtcacgccag agggtatcgc cagccagata gagcgttttc
    14101 tctgtcgggt gctggaaaac aacgccgcaa gctgcgccta aacgcttcgc catcgccggg
    14161 tcggcatagg tgcgatcgga gccgtgctgc cctgtcgttt tatgcagagt aatatcgcca
    14221 aatttagtct cgtcggtaag tacggtaaga tgcgtaaatc cttgtttatt cagtgattca
    14281 gcatcttgct cattttgcac ataaatcagt ttatctttag ggacgacgcg ggaggctgtt
    14341 tcgtcccagt gatcttcatg caaatgggtc acgattaggg cgtcaacgtt gagtaacgtt
    14401 gttaaatcgc acggtaattc taccgttgga ttgcggattt ctgcgcgtgc cgttccggca
    14461 aaacccggat aacttccttt aggcgcgagt aacgggtcga taagaaatgt tttgccagcg
    14521 tattggataa tttgggtggc attacgaatt tgcgtaattt tcatgatttg ctccacatca
    14581 cagtattgat gtcattagcg tagcgtgcag cccgtactgt gcatgtgggc aaatgggcga
    14641 ataacgatga gaatgggcca aattacacat tggcgtaact agcgtaaatg cacgccgctt
    14701 cgccgtccgg ccaccagaat agcctgcgat tcaacccctt cttcgatctg ttttgctacc
    14761 cgttgtagtg ccggaagatg cttttccgct gcctgttcga tggtcattgc gctcgccata
    14821 tacaccagat tcagacagcc aatcacccgt tgttcactgc gcagcggtac ggcgatagag
    14881 gcgatcttct cctcctgatc ccagccgcgg tagttctgtc cgtaaccctc tttgcgcgcg
    14941 cgcgccagaa tggcttccag ctttaacggt tctcgtgcca gttgatagtc atcaccgggg
    15001 cgggaggcta acatctcgat taattccttg cgttcttgtt ccgggcaaaa ggccagccag
    15061 gtcaggcccg aggcggtttt cagaagcggt aaacgccgcc cgaccattgc ccggtgaaag
    15121 gataagcggc tgaaacggtg agtggtttcg cgtaccacca ttgcatcaac atccagcgtg
    15181 gacacatctg tcggccatac cacttcgcgc aacagatcgc ccagcagtgg ggccgccagt
    15241 gcagaaatcc actgttcgtc acgaaatcct tcgcttaatt gccgcacttt gatggtcaga
    15301 cgaaaactat catcggaggg actacgccgg acatatccct cttcctgcag cgtctccagc
    15361 agtcgccgca cagtggtgcg atgcaggccg ctgagttccg ccagcagccc gacgctggca
    15421 ccgccgtcaa gtttatttaa catatttaat aacattagac cgcgggttaa gccgcgcacg
    15481 gttttgtatt ccgtctgctc attgttctgc atattaattg acatttctat agttaaaaca
    15541 acgttgtgca cctggtgcac attcgggcat gttttgattg tagccgaaaa caccctttct
    15601 atactgagcg cacaataaaa aatcatttac atatttttaa caaaataagt tgcgctgtac
    15661 tgtgcgcgca acggcatttt gtccgagtcg tgaggtactg aaatggcaat acaacaccct
    15721 gacatccagc ctgctgttaa ccatagcgtt caggtggcga tcgctggtgc cggtccggtc
    15781 gggctgatga tggcgaacta tctcggccag atgggcattg acgtgctggt ggtggagaaa
    15841 ctcgataagt tgatcgacta cccgcgtgcg attggtattg atgacgaggc gctgcgcacc
    15901 atgcagtcgg tcggcctggt cgataatgtt ctgccgcata ctacgccgtg gcacgcgatg
    15961 cgttttctca ccccgaaggg ccgctgtttt gctgatattc agccaatgac cgatgaattt
    16021 ggctggccgc gccgtaacgc ctttattcag ccgcaggtcg atgcggtgat gctggaaggg
    16081 gtgtcgcgtt ttccgaatgt gcgctgcttg ttttcccgcg agctggaggc cttcagtcag
    16141 caagatgacg aagtgacctt gcacctgaaa acggcagaag ggctgcggga aatagtcaaa
    16201 gcccagtggc tggtggcctg tgatggtggg gcaagttttg tccgtcgcac cctgaatgtg
    16261 ccgtttgaag gtaaaactgc gccaaatcag tggattgtgg tagatatcgc caacgatccg
    16321 ttaagtacgc cgcatatcta tttgtgttgc gatccggtgc gcccgtatgt ttctgccgcg
    16381 ctacctcatg cggtacgtcg ctttgaattt atggtgatgc cgggagaaac cgaagaacag
    16441 ctgcgtgagc cgcaaaatat gcgcaagctg ttaagcaaag tgctgcctaa tccggacaat
    16501 gttgaattga ttcgccagcg tgtctacacc cacaacgcgc gactggcgca acgtttccgt
    16561 attgatcgcg tactgctggc gggcgatgcc gcgcacatca tgccggtatg gcaggggcag
    16621 ggctataaca gtggtatgcg cgacgccttt aacctcgcat ggaaactggc gttggttatc
    16681 caggggaaag cccgcgatgc gctgctcgat acctatcaac aagaacgtcg cgatcacgcc
    16741 aaagcgatga ttgacctgtc cgtgacggcg ggcaacgtgc tggctccgcc gaaacgctgg
    16801 cagggtacgt tacgtgacgg cgtttcctgg ctgctgaatt atctgccgcc agtaaaacgc
    16861 tacttcctcg aaatgcgctt caagccgatg ccgcaatatt acggcggtgc gctggtgcgt
    16921 gagggcgaag cgaagcactc tccggtcggc aagatgttta ttcagccgaa agtcacgctg
    16981 gaaaacggcg acgtgacgct gctcgataac gcgatcggcg cgaacttcgc ggtaattggc
    17041 tggggatgca atccactgtg ggggatgagc gacgagcaaa tccagcagtg gcgcgcgttg
    17101 agcacacgct tcattcaggt ggtgccggaa gtgcaaattc ataccgcaca ggataaccac
    17161 gacggcgtac tacgcgtggg ggatacgcaa ggtcgcctgc gtagctggtt cgcgcaacac
    17221 aatgcttcgc tggtggtgat gcgcccggat cgctttgttg ccgccaccgc cattccgcaa
    17281 acactgggca agaccctgaa taaactggcg tcggtgatga cgctgacccg ccctgatgcc
    17341 gacgtttctg tcgaaaaggt agcctgatat gcacgcttat cttcattgtc tttcccactc
    17401 gccgctggtg gggtatgtcg acccggcaca agaggtactc gatgaggtca atggcgtgat
    17461 tgccagcgcc cgcgagcgta ttgcggcttt ctcccctgaa ctggtggtgc tgtttgcgcc
    17521 agatcactac aacggctttt tctatgacgt gatgccaccg ttctgtttag gcgttggagc
    17581 gacggcaatt ggtgatttcg gcagtgcggc aggagagctg cccgtgcctg tggagctggc
    17641 ggaggcctgt gcgcatgccg tcatgaagag cgggatcgat cttgccgttt cttactgtat
    17701 gcaggtggac cacgggttcg cccagccgct ggagttcctg ctcggtgggc tggataaggt
    17761 gccagttctg cctgtgttca tcaacggtgt cgccacgccg ctgcccggtt tccagcgtac
    17821 ccgcatgttg ggtgaagcca ttggacgttt caccagcact ctcaataaac gcgtgctgtt
    17881 cctaggttcc ggtgggcttt cccatcagcc gccggtgccc gaactggcga aagccgatgc
    17941 ccatatgcgc gaccgcctgt tggggagcgg gaaagattta cccgccagtg agcgcgaatt
    18001 gcgtcagcaa cgggtgatta gcgccgctga gaagtttgtt gaggatcaga gaactctgca
    18061 tccgctcaac ccgatttggg ataaccagtt catgactttg ctggagcagg gacgcataca
    18121 ggaactggat gccgtcagta acgaagagct ttccgccatt gccggaaagt cgacacatga
    18181 aatcaaaacc tgggtcgccg cttttgccgc tatttctgca tttggcaact ggcgtagcga
    18241 agggcgttat taccgcccaa tcccggagtg gattgccgga tttggctcgt taagcgccag
    18301 aacagagaac tgaatatgca ggagaagatg atgagttatc agccacaaac cgaagccgcc
    18361 accagccgtt ttctgaatgt agaagaagcg ggtaaaacgc tgcgcatcca ttttaatgac
    18421 tgcggacaag gcgacgaaac cgttgtcctg ctgcatggtt ccggcccggg tgctactggc
    18481 tgggcgaact tcagccgcaa tatcgatccg ctggtagagg cgggctatcg ggtgatcctg
    18541 ctggattgtc cgggttgggg caagagcgat tcgatcgtta atagtggttc gcgatcggat
    18601 cttaatgcac gaatcctgaa aagcgtggtg gatcaactgg atatcgccaa aatccacctg
    18661 ctgggcaact cgatgggtgg ccatagttct gtggcgttca cccttaactg gccggagcgc
    18721 gtcggcaaac tggtgctgat gggcggcggt acgggcggca tgagtttgtt tacgccgatg
    18781 ccaaccgaag gtattaagcg actgaatcag ctttatcgtc agccgactat cgaaaacctg
    18841 aagctgatga tggatatctt cgtttttgat accagcgatt tgaccgacgc cctgtttgaa
    18901 gcgcgcctga ataatatgct gtcgcgccgc gatcacctgg aaaacttcgt taagagcctg
    18961 gaagctaatc cgaaacagtt cccggatttt ggcccacgtc tggcggaaat caaagcgcaa
    19021 accctgattg tctgggggcg caacgaccgc tttgtgccga tggatgcggg tctgcgtctg
    19081 ctgtccggca ttgccggttc tgaactgcat atcttccgcg actgtgggca ttgggcgcag
    19141 tgggaacatg ccgacgcttt caatcaactg gtgctgaatt tcctcgcacg tgcttaagga
    19201 acggtcatga cgaagcatac tcttgagcaa ctggcggcgg atttacgccg cgccgcagag
    19261 cagggcgaag cgattgcgcc gctgcgcgat ctgattggta tcgataacgc tgaagcggcc
    19321 tacgccattc agcacataaa tgtgcaatat gacgttgcgc aggggcgtcg cgtggtgggg
    19381 cgtaaagtgg gcctgacaca tccgaaagtg cagcaacaac tgggcgttga tcaaccggat
    19441 ttcgggacat tatttgccga catgtgttat ggcgataacg aaatcattcc tttttcccgt
    19501 gttctgcaac cccgcattga agcggagatc gcactggtgt tgaaccgcga tttacccgca
    19561 actgatatca ccttcgacga attgtataac gccattgaat gggtacttcc ggcgctggag
    19621 gtggtgggta gccgcattcg cgactggtcg attcagtttg tcgataccgt ggcagataac
    19681 gcttcctgcg gggtgtatgt catcggcggt ccggcgcaac gtccggcggg gttagacctg
    19741 aaaaactgcg ccatgaagat gacgcgtaat aacgaagagg tttccagcgg gcgcggcagc
    19801 gaatgcctgg gacatccgct taatgccgcc gtctggctgg cacgcaaaat ggccagtctg
    19861 ggtgaaccgc tgcgcgccgg agatatcatt cttaccgggg cattaggtcc gatggtggca
    19921 gtgaatgcgg gcgatcgttt tgaagcccat attgaaggca taggttcagt tgctgcggca
    19981 ttttcaagcg cagccccaaa aggaagtctg tcatgagtaa gcgtaaagtc gccattatcg
    20041 gttctggcaa cattggcacc gatctgatga ttaaaatttt gcgtcacgat cagcatctgg
    20101 agatggcggt gatggtcggc attgatcctc agtccgacgg tctggcgcgt gccagacgta
    20161 tgggcgtcgc caccacccat gaaggggtga tcgggctgat gaacatgcct gaatttgctg
    20221 atatcgacat tgtatttgat gcgaccagcg ccggtgctca tgtgaaaaac gatgcggctt
    20281 tgcgcgaagc gaaaccggat attcgcttaa ttgacctgac gcctgctgcc atcggccctt
    20341 actgcgtgcc ggtggttaac ctcgaggaga acgtcgatca actgaacgtc aacatggtca
    20401 cctgcggcgg ccaggccacc attccaatgg tggcggcagt ttcacgcgtg gtgcgtgttc
    20461 attacgccga aattatcgct tctatcgcca gtaaatctgc cggacctggc acgcgtgcca
    20521 atatcgatga atttacggaa accacttcgc gagccattga agtggtgggc ggcgcggcaa
    20581 aagggaaggc gattatcgtg cttaacccag cagagccacc gttgatgatg cgtgacacgg
    20641 tgtatgtatt gagcgacgaa gcttcacaag atgatatcga agcctcaatc aatgaaatgg
    20701 ctgaggcggt gcaggcttac gtaccgggat atcgcctgaa acagcgcgtg cagtttgaag
    20761 ttatcccgca ggataaaccg gttaatttgc cgggcgtagg gcagttctcc gggctgaaaa
    20821 cggcggtctg gctggaagtc gaaggcgcag cgcattatct gcctgcctat gcgggcaacc
    20881 tcgacattat gacttccagt gcgctggcga cagcagaaaa aatggcccag tcactggcgc
    20941 gcaaggcagg agaagcggca tgaacggtaa aaaactttat atctcggacg tcacgttgcg
    21001 tgacggtatg cacgccattc gtcatcagta ttcgctggaa aacgttcgcc agattgccaa
    21061 agcactggac gatgcccgcg tggattcgat tgaagtggcc cacggcgacg gtttgcaggg
    21121 gtccagcttt aactatggtt tcggcgcaca tagcgacctt gaatggattg aagcagcggc
    21181 ggatgtggtg aagcacgcca aaatcgcgac gttgttgctg ccaggaatcg gcactattca
    21241 cgatctgaaa aatgcctggc aggctggcgc gcgggtggtt cgtgtggcta cgcactgtac
    21301 cgaagctgat gtttccgccc agcatattca gtatgcccgc gagctcggaa tggacaccgt
    21361 tggttttctg atgatgagcc atatgaccac gccggagaat ctcgcaaagc aggcaaagct
    21421 gatggaaggc tacggtgcga cctgtattta tgtggtggat tctggcggtg cgatgaacat
    21481 gagcgatatc cgtgaccgtt tccgcgccct gaaagcagtg ctgaaaccag aaacgcaaac
    21541 cggcatgcac gctcaccata acctgagtct tggcgtggcg aactctatcg cggcggtgga
    21601 agagggctgc gaccgaatcg acgccagcct cgcgggaatg ggagcgggcg caggtaacgc
    21661 gccgctggaa gtatttattg ccgccgtgga taaactgggt tggcagcatg gggccgatct
    21721 ctatgcgtta atggatgctg ctgacgacct ggtgcgtccg ttgcaggatc gaccggtacg
    21781 agtcgatcgc gaaacgctgg cgctgggata cgctggtgtt tactccagct tcctgcgtca
    21841 ctgtgaaacg gcggcggcgc gttatggctt aagcgcggtg gatattctcg ttgagctggg
    21901 caaacgccgg atggttggcg gccaggagga tatgatcgtc gacgtggcgc tggatctgcg
    21961 caacaacaaa taatattgac tgccgagaac gtgcattttg tatgcccatc ccagcacgac
    22021 tgccggatgc ggcgtgaacg ccttatccgg tctacatttc gcccgtaggc agtcattaaa
    22081 tagttctgat tacgcggctg gcaatgtatc agtcgcgatt cacctgcaca cgcaatgagg
    22141 ttcatcttat gtcgactcgt accccttcat catcttcatc ccgcctgatg ctgaccatcg
    22201 ggctttgttt tttggtcgct ctgatggaag ggctggatct tcaggcggct ggcattgcgg
    22261 cgggtggcat cgcccaggct ttcgcactcg ataaaatgca aatgggctgg atattcagcg
    22321 ccggaatact cggtttacta cccggcgcgt tggttggcgg aatgctggcg gaccgttatg
    22381 gtcgcaagcg cattttgatt ggctcagttg cgctgtttgg tttgttctcg ctggcaacgg
    22441 cgattgcctg ggatttcccc tcactggtct ttgcgcggct gatgaccggt gtcgggctgg
    22501 gggcggcgtt gccgaatctt atcgccctga cgtctgaagc cgcgggtcca cgttttcgtg
    22561 ggacggcagt gagcctgatg tattgcggtg ttcccattgg cgcggcgctg gcggcgacac
    22621 tgggtttcgc gggggcaaac ttagcatggc aaacggtgtt ttgggtaggt ggtgtggtgc
    22681 cgttgattct ggtgccgcta ttaatgcgct ggctgccgga gtcggcggtt ttcgctggcg
    22741 aaaaacaggc tgcgccacca ctgcgtgcgt tatttgcgcc agaaacggca accgcgacgc
    22801 tgctgctgtg gctgtgttat ttcttcactc tgctggtggt ttacatgttg atcaactggc
    22861 taccgctgct tttggtggag caaggattcc agccatcgca ggcggcaggg gtgatgtttg
    22921 cactgcaaat gggtgcggcg agcgggacgt taatgttggg cgcattgatg gataagctgc
    22981 gtccagtaac catgtcgcta ctgatttata gcggcatgtt agcttcgctg ctggcgcttg
    23041 gaacggtgtc gtcatttaac ggtatgttgc tggcgggatt tgtcgcgggg ttgtttgcga
    23101 caggtgggca aagcgttttg tatgccctgg caccgttgtt ttacagttcg cagatccgcg
    23161 caacaggtgt gggaacagcc gtggcggtag ggcgtctggg ggctatgagc ggtccgttac
    23221 tggccgggaa aatgctggca ttaggcactg gcacggtcgg cgtaatggcc gcttctgcac
    23281 cgggtattct tgttgctggg ttggcggtgt ttattttgat gagtcggaga ttaagaatgc
    23341 agccgtgtgc cgatgcctga cgcgccttat taagcagggg cgaaagggac ttgcccccaa
    23401 tacacccacg gcgtttacag gtatactcgc taaaaattat tcagcgggtt tggaaacaaa
    23461 gatggcaaaa cttaccttac aagagcagtt gctcaaagca ggattagtca ccagcaaaaa
    23521 agcggcgaag gtcgagagaa cggcgaaaaa atcgcgcgtt caggcgcgtg aagctcgggc
    23581 ggcggtagaa gaaaataaaa aggcacagct tgagcgtgat aaacagctta gcgaacagca
    23641 aaaacaagcg gcgttggcga aagaatataa agctcaggtg aagcagctta ttgaaatgaa
    23701 ccggatcacc attgccaatg gcgatattgg ttttaacttc actgatggca atctgattaa
    23761 gaagattttt gtcgataagc tcacacaggc gcagttgatt aatggtcgtc tggcgattgc
    23821 ccgcttgttg gtcgataaca atagcgaagg tgaatacgcc attattcccg ccagcgttgc
    23881 cgataaaatt gctcagcgtg atgccagcag tattgtgttg cacagcgcgc tcagtgccga
    23941 agagcaggat gaagacgacc cgtatgccga cttcaaagtg cctgatgatt tgatgtggta
    24001 aacatagata agtcgtgcgc agatgcctga tgcgacgcta tgcgcgtctt atcatgccta
    24061 cggcctgcgg gcgaaatgta ggcggataag gcgttcacgc cgcatccggc agtcgtgcac
    24121 tattatcaac gcatattcag tttattggcg tggtaggcaa tatgctcgcc aataaaactg
    24181 gagacaaaat aatagctgtg gtcgtaaccc tcgtgataac ggattaacgt tttgatattc
    24241 atctcctggc agatcttttc aagattttga gtccgcagct gttccgcgta aaaatcatca
    24301 ctcaacccct gatcaaccat gatttccgca acgcgttgac cttgtgaaat aagactcacc
    24361 ggatcgtaat ccaaccaggc ctctttattt tcaccaagat atgcggcaaa ggcttgctgt
    24421 ccccacggta cttgcgatgg ggagacaatg ggcgaaaacg ccgagacgct gacatattca
    24481 tctgggttac gtaacgccag caccagcgcg cccagtccgc ccatagaatg accagagata
    24541 gactttttgg ccgttgccgg aaaatgattc atcactaaat ccggcagctc gttgcggata
    24601 tagtcataca ttttgtaatg ttcattccac ggcgcttgcg tcgcgttcag gtaaaacccg
    24661 gcaccttgcc cgagatcgta acggtcggca tctgcgacat gactgcctcg cggactggtg
    24721 tccggcgcaa caacaataat gttgtgctca gccgcgtaac gctgcacccc cgatttagta
    24781 atgaaattct gctcattgca ggtcaggccc gaaagccagt acagcaccgg taatttttca
    24841 cctgcagctt ttggtgggag atagacgccg acattcattt cacatttcag tgatgcggaa
    24901 taatgccgat acacattttg ccagccgcca aagctggcat gtttttcaat gagttccatc
    24961 agtcattatc tcacggttaa tcccccggac ggctgcccgg agagagtatt aagaaccccg
    25021 tctgaagtgg acggggtaaa ccagcgggaa atcagtaacg aattacggtt cgaatggatt
    25081 tgccttcatg catcaggtcg aaggcgtcat taatttcatc caggctcatg gtatgcgtga
    25141 caaacggttc cagatcgata tcacctttca tcgcatcttc aaccataccc ggtaactggg
    25201 aacgaccttt cacgccgcca aacgcggaac ctttccatac gcgaccagtg accaactgga
    25261 atggacgggt ggagatttcc tgaccggaac ccgcgacccc gatgatcacc gactgacccc
    25321 agccgcggtg cgcactttcc agcgccgcac gcatcacgtt gacattaccg atgcattcaa
    25381 aggtatggtc gataccccat ttgttgatat ccagcaggac atctttaatc ggtttgtcgt
    25441 agtcattcgg gttaatgcag tcggtggcac cgaagcgacg cgccagatcg aatttcttcg
    25501 ggttggtatc gatagcgata atccgtcccg ctttcgcctg acgcgcgccc tgaaccactg
    25561 ccagaccaat cgcgccaaga ccaaacacag caacagaatc ccctggctgg actttagctg
    25621 tgttgtgtac cgcgccaata ccggtggtca cgccacagcc cagcaggcag acgtgttcat
    25681 ggtttgcttc tggattaatt tttgccagag acacttccgc gactacggtg tattcactga
    25741 atgtagaaca ccccatgtag tgataaagcg gctgcccatt gtaagaaaaa cgggtggtgc
    25801 cgtctggcat caggccttta ccctgggttt cgcgaaccgc aacacagagg ttagttttgc
    25861 cagaacgaca gaactcacac tcgccgcact ctgcggtgta aagcgggatc acatggtcgc
    25921 ctggtttgac gctggttacg ccttcaccga cttcaaccac aacgccagcc ccttcgtgac
    25981 cgagaaccac cgggaataca ccttccgggt catcgccgga gagggtaaat gcgtcggtat
    26041 ggcaaacgcc ggtatgggtg actttaatca gcacttcacc ttttttcggt ggtgcaacgt
    26101 caatttcaac gatttccagc ggtttaccgg gagcaaatgc aacggcagca cgtgatttca
    26161 tctctcgctc ttcctcaata tggtaataga ttcagctatt taagataggc acgaaccagt
    26221 tcaatagtgt cgtcaacgga ttggctgact tcgcggctgt agcagtcatt tcggtcaaac
    26281 gtttcccgga tatggctttc aagcacttct gccatcagcc cattagccgc gccccgaacg
    26341 gcagcgatct gttggagtat ggcacggcat tcggcatcac cctccagcga ccgttccaga
    26401 gcatcaatct gccctcgaat acgacgaact cgagtaagga cctttttctt ctcttccgga
    26461 gtactgggca tttcgcacct catcatctgc acgcaatata ctataggggg gtatgctata
    26521 tgtcaatgca taccccccta tagtatataa caatggagaa taattgttgt ccgaagccta
    26581 accaggtgaa cggtccctca agacagatgg ggaccgttta tgtgctattt ttatgccgca
    26641 tccgctaaaa caaacatccc gtaaggatgg atttcgaggt aatagtgctc accaatatcg
    26701 ggctgtaaac gggtggcgtt tacttgtaat aaaatctcct gtccatgcca ctctacggtc
    26761 acttcgtact gcggccccat gtaggcgaca tggcgaataa cacaacgctg actctcttcg
    26821 ccgtgattgc ttaaggtgat ggcttccggg cgaacgccga cggtgccgct accttgcacc
    26881 gggaaatgcg ccgcgcgcgg caggcgataa ccgtagatat cgacatactc ttcgctaaag
    26941 ttcgccggga acaggttggc gtcgcccata aaacttgcca taaagcggga ggctggctga
    27001 cgatagagat cctgcggtga gccaatctgc atgatgtgac ctttattcat caccagcaca
    27061 gtgtcggaaa ccgcgaaggc ctcactttga tcgtgagtca cgtagagcga cgtgatatta
    27121 aactgctttt gcagctcgcg gatcttatcg cgcatgctgc ggcgcaggtt ggcatcgagg
    27181 ttacttaacg gctcatcaaa cagcagcacc tttggtttga ggatcaacgc gcgggccagc
    27241 gccacgcgct gttgctgacc accggaaatc tggtcgacat agcggtcctc gaacccttcc
    27301 agatccacca ttgccagcgc ctctttcacc cgctgcttta cttcgctacg cgacacgccg
    27361 agcatcttta acccgtagcc gacgttttcg cccagcgaca tatgcgggaa cagagcgtat
    27421 gactgaaaca ccatgcagat atcgcgctgc tgaatggaac gatgcgtgac atcttcgcca
    27481 tcaataaaga tttgcccttc actcggtttt tccagcccgg caaccagacg caaaatggtg
    27541 gttttgccgc agccggaagg gccaagcagc gtcaccattt gcccttgtgg gatggtgaga
    27601 ttgatattgt cgataaccgt attactgccg aatcgtttag tgacgttgcg cagttccaca
    27661 aaatttttct gactcatagt gcgctccatt acgcctggtt tttggcttta gaacgggaga
    27721 tacgtgcttc tccgatcagc cagtcaaaga ggaaaataat cgccagcatc accacgatca
    27781 gaatcgaacc ataggcgatg gctacgccat attcgccgtc ttcaacgcgg ttaagaatgt
    27841 aggcggttgc cacgcgggtg tccggcgtga cgagaaagac gatggcgctg accgtcgtga
    27901 tggcccgcac aaagctgtag atcagggccg agagaatggc cggacgcagc agcggcagta
    27961 aaatgtgggt tatggttcgc aaggaacccg cgcgcaggct gagtgaggct tcatccagcg
    28021 atttgtcgat ctgccccagg cctgcaatcc cggcgcgaat acccaccgga acgttacgca
    28081 tcaccatcga gataatcaca attgccgccg ttccggtgag ataaaccgga gcgctgttaa
    28141 aggcaagaat atacgaaacg cccgccaccg tacccggtac ggcaaagcac agcatggtgg
    28201 tgaactcgat ggtctttttg cctttgaatt gttgacgcac caccacccag gcgatgagca
    28261 ggccaaagat agcggtaatg ggagcggcaa tcccggcgta gagcagggta tccagcagtg
    28321 aaggccacgc gccgtcgctc attccctgac caaatagctt gataaagtta tccagcgtca
    28381 gggtgtagtc cacgccccag ttcaccgtga agctgccgta gaaaatgctg ccgtagagca
    28441 gggcgttaaa ggcaatccat atcgcaagta tggcgaccac gctccacacc agggtgaccg
    28501 gaagcggctg gacgtcgcca cggtaggatt tgccggaaac cgtgacgtaa gagcgtttac
    28561 caatccacat atactgtatg cagaacacca gcagcgagaa cagcagcagg aaagcgccaa
    28621 gggtgctggc ggcctgataa tccagctgcg agccggtaat atagaagtaa atttgcgtcg
    28681 ccagtacgtc gaagttaccg ccaagtacca gcgggttact gaaatcagcc agtgactgta
    28741 ccacgacgat taaaaaggca ttggcgagcg cgggcttcaa caacggcaca aacacgccgt
    28801 taaaggtttg ccagcggctg gcgcgcaggg tgtaggaggc ctcttccagc gagggatgaa
    28861 tggttttaat ggccccgtcg aggatcataa atgccattgg cgtgaatgcc agaacctgtg
    28921 ccagccagat cccggtaaaa ccgtacagcc agttggtgtt ggtcagccca aaccactcca
    28981 ccataaattc cgtaacatag ccggaacgcc ccatcatgag cgtcacgcct aagccgacca
    29041 caaacggtgg cgtaacgatg ggcaaaatgg aaaagatgcg accaataatg gcgctgcgtt
    29101 tagcgatgcg cgtggtataa atcgccagca ccaggccgaa gaaagcacag cctatcccca
    29161 ccgctatcga caagacgata gagttgacga tcacctgaac aatatgtgcc tgcgagagca
    29221 cggtcataaa cgccagcggg gcgagctctc ccgcgtcgtt ggtgaacatc gggataaaaa
    29281 tggcgatgct cggccagacg ataaaaatgc caatcagcgc cacaatcgca atcagtgagc
    29341 cgatcacgaa gcggtcgccg cccagccatt cgaggcgggt taaggccagc gtcataatcg
    29401 cgcctagttc gacaaacagc acgatggcgg cgtagcctaa accgcgtcca gccacggtgg
    29461 cgctgatgac gataaacgcc atacagagca atgcccagcc ggcatcgaaa taatgacgac
    29521 tacgctgttc gcgcgtggcc tgacgaaacg ggcggaccag caacaggctc gggagtaaat
    29581 accacagcca gctgatatta aaatgcgacc agccgtacgc cccgaggatc tcatcactcg
    29641 tggattccag caggccataa tcgaggctcc agctcggcaa caggacaaat gccagccagc
    29701 ccagcaaaac ccaaaggaaa atcgcatccc gctttttcac gggatggagt gcaagtgtct
    29761 gtgacatagc agttccggta tggttggccg gggcaggcgt ctgcgctgca cccggcaaat
    29821 gcgcctaatg cgggtttatt tacccatctt cacttcgctg acccatttat taatcaatgc
    29881 cttacgaaca tccgtggaac catatttatc catgtcgtag ttgattaatt tcaggtcgtc
    29941 gagcttcagc gaatttggcg acgtttcggc ggtggtatta gtcaggatct gataagactt
    30001 ccctttcttc cacgccagtt cctgagcttc ttttgataat acccagtcca caaacagttt
    30061 ggcgttgtcg aggttgcgcg cgcctttcag aatgctgacg ccgccaattt cgtagcccgt
    30121 accttcacac ggggaaatca actccagcgg cgcgccctgt tctttttcca gcgagtagtc
    30181 atgcaggaag ccaataccaa tcgccgtttc gccacgggcg gcgttacgtg ccggggctat
    30241 gcctgatttg gtgtactgag agacgttggc gttcagttgt tttagataat caaaggcctg
    30301 atcttccccc cacagctgcg caaaagtagc cagggcggtg taggcagtac cggaactttg
    30361 cggatcggca atttgaattt cgcctttata ttccggtttg gtgagatctt tccagcactt
    30421 cggcaccggc aggtttttct ctttcagacg atcggtgttt acgccaaaac cgaggatccc
    30481 cacataaacg gcagaggaca gattcccttt cacttttgcc ggatcgcgga atttctccat
    30541 gatctgctca aggttagggg atttatacgc ctgtagcagg cccatctcgc cagcctgcga
    30601 ttgcgggtct aaagtgccgc cgtaccagac gtcagcctgg gggtttttct tttcagcatc
    30661 gacttttgcc agggtgcttc cggaaccgtt gcggataaac gaggttttta catcatattt
    30721 ctcgccaaac gctttggttt cggcctcaca catttcattg gtggcgctgc aatagaccac
    30781 cagccggcct tttgcctgcg cggcacccgt taaagtcgcc agcgcaacgg cagaagcaat
    30841 cagagtagag gtaaaggtca ttttcattgt tttatccttc gagcgtgagt ggtggtgatg
    30901 cccgccatca gtaacggcat cagcagtaaa cccataagta ctgcggcgac cgagagtaaa
    30961 ctgaacattc ccggccagcc gtagcgttcg atgaccagcg acagcggcca gcctgccagc
    31021 gccgcgccaa ggtaagcgaa caggccgaga aatccggtga ttgaacccgc cgcagctttg
    31081 tgcccacact ccaccgccgc aagtccaatc agcatttgtg ggccaaagac gaaaaagccc
    31141 acggtaaaaa agcacactgc cagcagagcg tagtgatgaa ccggtgccag ccacagtgcg
    31201 gcgactgaca ccatcaaccc caacgtgaac agcaaaatca ttggcgctcg ttgcccgcta
    31261 aacagcaaat ccgatcccca tccggcaaat aacgcgccga gtaagccgcc cacttcaaac
    31321 agcattaccg tggcgttggc gctgagcagg ttgacaccgt ggctttccgt cagccagata
    31381 ttgccccagt cgttaagcgc aatccggatc acataaacca gtacatatga gacgcccagt
    31441 agccagatca gcggattttg cagcatggtg gtgcgcaaca tttgccacag ccccatcggc
    31501 ggactttgtt gttcctgacg taactccagc ggatcgtgac gccagtgacc aactgtcggt
    31561 aatccctctt cctggggcgt gcctttgagt tgtagcgtta accagatccc taatgccatg
    31621 ctgattatcc ccggtgtcag catcgcggcc tgccagcccc accagtgggc ggcaaaggcg
    31681 ctaatcagcg gaataatcgc tccgccaatg ttgatcgaca tattccagca gccccaccag
    31741 aaaccgcgct cattgcgtga gtaccagtgg gtcagcaagc gggcacacgg tggccatccc
    31801 cagccctgaa aaaaaccgtt cagtgtccag accacaagca gaagcgtaag tgattcgcca
    31861 aaggcaaaca ccacgttcaa taacccggtg gcgaacaagc cgacgcccat aaaaccgcgt
    31921 tgtccgtggc tgtcatgcca cagtccggcg gtaaatttcg acaatccgta gctgagataa
    31981 aacagcgaac ccagcaggcc aatgtcgcct ttatccagtc cgaggtcggt ttgtagcgcc
    32041 ggaaggacgt aattcacgct ttttcgcgtc agataaaatg cggcgtagcc aataatcatg
    32101 cacatcagca atcgcggacg taatgtccgg tagcattgat taatttctct cgctgaacgc
    32161 gcgtgcatgg ttgtctccat gttgctgact atatgaaaat gaaagtgaaa ggggatgaga
    32221 gaaaatccgg gatacctaag actttttcct ggttatccgg cgatttgttg caaaaatgtg
    32281 ggcaagttaa caattacccg cgtgccgtgc tgtttttcga gcgttaagtc gccgcccagg
    32341 gcgctgacgc gctcacgcat cccctgaatg ccaaatccag ccattttccc gctggcgatc
    32401 cccacaccgt tgtcgctgac ttccagatgc aatacctctc cctgctggcg caggataatc
    32461 gtcacttcgc tggcttcggc gtgtttacag atgttattga gcaactcctg caataaccga
    32521 tacaaggtaa agcgcaccgt ttcgttttct ggtgtggagt tcagttgata ggcaaactgg
    32581 caatgaatgc cgcgttcact aaaggcaaat tcattaatca gatgcagcaa cgcttcgcga
    32641 aatgtcagtt catcaagcgc gggaggccgc aactggcgca gcaattggcg ggtggagaga
    32701 tgaatgcggc gggcgagctc attgatttgg ctggctgcgg attgaatttg cgccggatca
    32761 cgcgcccgct tcaccagttg cgactggatc tgaatggcgg tgatgttctg accgatttcg
    32821 tcgtgcagct ctcgcgccag gttttttcgc gtgtcctcct cggtgtggat cagcttttcc
    32881 gccaatgccc gccgcgctgc cagctcggcc tccagccgtt gtcggtaatg gtgcaggttt
    32941 agcgccagat gttgctggcg actgatggca atgcccaggc caattcccag caaggcctgg
    33001 gtcgcgagga aaatctccag ctccaccagg ttactaaagc ccacgccaat ctggcgggcg
    33061 atggtgatca tcatgctgcc caaaagcccg gagagtacgc caccttgcca gccaaatttc
    33121 cacgccatca ccacattggg caaaaagacc acgatcaaca gcagacgttc aatttcaggt
    33181 gacagcacca tctgagtgcc gataccaata ataaaaaaca ggctacacca gatgattaaa
    33241 gaggtacgca aggggggatt atgcgtatcg agtcccagga gctggtagcg atgctgctgg
    33301 cgcagaaact caaagataag atagacaaac ggcgtcagta atacgccgcc ggtaaatgag
    33361 gtcaaacccg gtagcattat tgggcctgac ataaaagggg agagcagggc ggtattcaac
    33421 aatgaagctg ccgtgacagt ggctaacagc aacgtcagac gctgccagta aagtggaaaa
    33481 cggtgccaga agttttgcgc aaggagggca ggaaacaggc taataaccgg cgttgccagc
    33541 atgagataac cgttcaatag ctgttcacta tggagccaga acatcatcag caaagggggg
    33601 agcaccaacg caggccagta gcggcgagag agcaaaataa caagggcgag ataaactccc
    33661 tgcgggagaa acaacaccgc ctgttgaccg ttattggtca gataaaagct gagcgtccac
    33721 agcatcagcc agccggttcc ccaggctagc acaataaaca gcaaaatcac ccaatgacga
    33781 agattgcgcg acattaatgc cccgccagca gatggtggtc gagcgcaaaa tgtaccagtt
    33841 caatggtgct atggcagttc agttttccca gcacattggc gcgatgcaca tgtaccgttt
    33901 tatgactgag ttcgagttta aaggcgatct ctttaacgct gtctcctttg accagtaact
    33961 caaacacttc acgttctcgt ggggtgagaa tttccagcgc ctgggcgggt tgcccgccgc
    34021 cgcgtaaagc ccgaatcgca tcggcacaga gataatgccc gcccagcccg accgagcgca
    34081 ccgcctgtac taactcttcc ggcccacagc gtttggttaa atacccgctg gcaccggcat
    34141 ccagcgcgct ttgcacaaag gttggcgcgt cataaatgct aagaataatg gcgcgaaatt
    34201 gcggtttttg tgcacgcagg cgcttaagca aactgaggcc gttttcgtcc ggcatggcaa
    34261 tatcaatgac ggcaacattg acgtcatcac ggattaatgc cgaccatgcc tgcgcggcac
    34321 tactgtactg accaatgacc tccagatcgt cttccagaga gagtaattgt gcgaagccag
    34381 agcgcaccac aacatggtca tccaccagca ctacccgaat catacttttc ttctcttatt
    34441 atctaattct cctatcatgc atcacggcgc ggcgctggca atcaaccagc gacagatatg
    34501 gaatggatgt aacaaaatgg caataatttg ccggagcgcg cacatcttat ctggcttgtg
    34561 aaccggcggg atacgttcgc caaaaccgca aaatgcgcaa actcgctaac cattgccgat
    34621 ttttttctaa aaacgctttg ttcttaacaa gaccttttaa ttcctttatc aaactgaccc
    34681 gcaacgaaat actgctccca taacaagaca ggggagcaga caatcatggc aatttcatcg
    34741 cgtaacactc ttcttgccgc actggcattc atcgcttttc aggcgcaggc ggtgaacgtc
    34801 accgtggcgt atcaaacctc agccgaaccg gcgaaagtgg ctcaggccga taacaccttt
    34861 gctaaagaaa gtggagcaac tgtggactgg cgcaagtttg acagcggagc cagcatcgtg
    34921 cgggcgctgg cttctggcga cgtgcaaatc ggcaaccttg gttccagccc gttagcggtt
    34981 gcagccagcc aacaggtgcc gattgaagtc ttcttgctgg cgtcaaaact gggtaactcc
    35041 gaagcgctgg tggtaaagaa aactatcagc aaaccggaag atctgattgg caaacgcatc
    35101 gccgtaccgt ttatctccac cacccactac agcctgctgg cggcgctgaa acactggggc
    35161 attaaacccg ggcaagtgga gattgtgaac ctgcagccgc ccgcgattat cgctgcctgg
    35221 cagcggggag atattgatgg tgcttatgtc tgggcaccgg cggttaacgc cctggaaaaa
    35281 gacggcaagg tgttgaccga ttctgaacag gtcgggcagt ggggcgcgcc aacgctggac
    35341 gtttgggtgg tgcgcaaaga ttttgccgag aaacatcctg aggtcgtgaa agcgttcgct
    35401 aaaagcgcca tcgatgctca gcaaccgtac attgctaacc cagacgtgtg gctgaaacag
    35461 ccggaaaaca tcagcaaact ggcgcgttta agcggcgtgc ctgaaggtga cgttccgggg
    35521 ctggtgaagg ggaataccta tctgacgccg cagcaacaaa cggcagaact gaccggaccg
    35581 gtgaacaaag cgatcatcga caccgcgcag tttttgaaag agcagggcaa ggtcccggct
    35641 gtagcgaatg attacagcca gtacgttacc tcgcgcttcg tgcaataaaa ggaggcgcag
    35701 atgctgcaaa tctctcatct ttacgccgat tatggcggca aaccggtact ggaagatatc
    35761 aacctgacgc tggaaagcgg cgagctactg gtggtgctgg ggccgtccgg ctgcggtaaa
    35821 accaccctgc tgaatttgat tgccggtttt gtgccttatc agcatggcag cattcaactg
    35881 gcgggtaagc gtattgaggg gccgggagca gagcgtggcg tagtttttca gaatgaaggg
    35941 ctactaccgt ggcgcaatgt acaggacaac gtggcgttcg gcctgcaatt ggcaggtata
    36001 gagaaaatgc agcgactgga aatcgcgcac cagatgctga aaaaagtggg gctggaaggc
    36061 gcagaaaaac gctacatctg gcagctttcc ggtggtcaac gtcagcgggt ggggattgcc
    36121 cgcgcgctgg cggcgaatcc ccagctgtta ttactcgacg aaccgtttgg tgcgctggac
    36181 gccttcaccc gcgaccagat gcaaaccctg ctgctgaaac tctggcagga gacgggcaag
    36241 caggtgctgt tgattaccca cgatatagaa gaagcggtgt ttatggcgac tgaactggtt
    36301 ctgctttcac ccggccctgg ccgcgtgctg gagcggctgc cgctcaactt tgctcgccgc
    36361 tttgttgcgg gagagtcgag ccgcagcatc aagtccgatc cacaattcat cgccatgcgc
    36421 gaatatgttt taagccgcgt atttgagcaa cgggaggcgt tctcatgagt gtgctcatta
    36481 atgaaaaact gcattcgcgg cggctgaaat ggcgctggcc gctctcgcgt caggtgacct
    36541 taagcattgg cacgttagcg gttttactca ctgtatggtg gacggtggcg gcgctgcaac
    36601 tgattagccc gctgtttttg ccgccgccgc aacaagtact ggcaaaactg ctcaccattg
    36661 ccggaccgca gggatttatg gacgccacgc tgtggcagca tctggcagcc agcctgacgc
    36721 gcattgtgct ggcgctactc gcggcggtgt tgatcggcat tccggtcggg atcgccatgg
    36781 ggctaagccc gacggtgcgc ggcattctgg atccgataat cgagctttat cgtccggtgc
    36841 cgccgctggc ttatttgccg ctgatggtga tctggtttgg tattggtgaa acctcgaaga
    36901 tcttactgat ctatttagcg atttttgcac cggtggcgat gtcggcgctg gcgggggtga
    36961 aaagcgcgca gcaggttcgc attcgtgctg cccagtcgct gggtgccagc cgtgcgcagg
    37021 tgctgtggtt tgtcattttg cccggcgcgc tgccggaaat cctcaccgga ttacgtattg
    37081 gtctgggtgt gggctggtct acgctggtgg cggcggagct gattgccgcg acgcgcggtt
    37141 taggatttat ggttcagtca gcgggtgaat ttctcgcgac tgacgtggtg ctggcgggga
    37201 tcgcggtgat tgcgattatc gcctttcttt tagaactggg tctgcgcgcg ttacagcgcc
    37261 gcctgacgcc ctggcatgga gaagtacaat gagtgaacgt ctgagcatta ccccgctggg
    37321 gccgtatatc ggagcacaaa tttcgggtgc cgacctgacg cgctcgttaa gcgataatca
    37381 gtttgaacaa ctttaccatg cggtactgcg ccatcaggtg gtgtttctgc gcgatcaagc
    37441 cattacgccg cagcagcaac gcgcgctggc ccagcgtttt ggcgaactgc atattcaccc
    37501 tgtttaccct catgccgaag gggttgacga gatcatcgtg ctggataccc ataacgataa
    37561 tccgccagat aacgacaact ggcataccga tgtgacattt attgaaacgc cacccgcagg
    37621 ggcgattctg gcagcgaaag agttaccttc gaccggcggc gatacgctct ggaccagcgg
    37681 tattgcggcc tatgaggcgc tctctgttcc cttccgccag ctgctgagcg ggctgcgtgc
    37741 ggagcatgat tttcgtaaat cgttcccgga atacaaatac cgcaaaaccg aagaggaaca
    37801 tcaacgctgg cgcgaggcgg ttgcgaaaaa tccgccgttg ctacatccgg tggtgcgaac
    37861 gcatccggtg agcggtaaac aggcgctgtt tgtgaatgaa ggctttacta cgcgaattgt
    37921 tgatgtcagc gagaaagaga gcgaagcctt gttaggcttt ttattcgccc atatcactaa
    37981 accggagttt caggtgcgct ggcgctggca accgaatgat attgcgattt gggataaccg
    38041 tgtgactcag cactatgcca atgccgatta cctgccacag cggcggatta tgcatcgggc
    38101 gacgattctt ggggataaac cgttttatcg ggcggggtaa tacgagagtg gacggtcctc
    38161 tcgccccctt ggggagaggg ttagggtgag ggggcgttca ccgtgctttc aacaggttaa
    38221 ctcccccttt ctgagaggaa acaaaattaa cgcagaatct tcttctcagc caaatccagc
    38281 gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa tcgaacctaa gctttcgagc
    38341 acgactttct cttcatctat agcacccgcc agcgcggcga acttaatcat cgcatactca
    38401 ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc acgcacgatg
    38461 tcgaggtacg ctccagcagg tttaaccatc aggcagtctg cgccctgggc ttcatccagc
    38521 agtgactcac gaatcgcctc acgacggttc attgggttca tctgatagct tttgcggtcg
    38581 ccttttaatg cgcttccggc agcttcacgg aacggaccat aaaaggagga ggcgaacttg
    38641 gtcgaatacg acataatcgc cgtatcttta aagccagcag cgtccagcgc ctggcgaatc
    38701 gcctgtacct ggccatccat cgcggcggaa ggggcgatga agtctgcacc tgcagcagct
    38761 gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac gccatgctcg
    38821 cacagcacac cgcagtgacc gtgagaagtg tattcacaga agcaggtgtc tgacatgaca
    38881 atcatttctg gcaccgtctg cttgcagatg cgcgacatac gcgctaccag tccatcttcc
    38941 cgccaggcat cgctgccggt ttcatcggta tggtgagaga tgccgaaagt catcacggaa
    39001 cgaataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc tggaatgcgc
    39061 atcacgccag gcatggcttc aacggctttg tagtcgtcaa tttcttcttc aacaaagatc
    39121 ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg cagcgcagga
    39181 gatttgcgca ggcgacgagg gcgttggatt aagtctgtca tggtctgcct gatgtttgtg
    39241 gaattaaggt gcacagtata cctgaagcgg ggctggagtg ttttacgaaa gttgtcacga
    39301 tgttgacaag aagagaatga aagagagacc agggcatctt tgtaatagaa
//
LOCUS       DAZFBR010000032        38697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFBR010000032 DAZFBR010000000
VERSION     DAZFBR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.032"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            113..364
                     /locus_tag="ACHJZ2_001899"
     CDS             113..364
                     /locus_tag="ACHJZ2_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4568023.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            399..586
                     /locus_tag="ACHJZ2_001900"
                     /pseudo
     CDS             399..586
                     /locus_tag="ACHJZ2_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(1061..1147)
                     /locus_tag="ACHJZ2_001901"
                     /pseudo
     CDS             complement(1061..1147)
                     /locus_tag="ACHJZ2_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1353..1679)
                     /gene="ynfA"
                     /locus_tag="ACHJZ2_001902"
     CDS             complement(1353..1679)
                     /gene="ynfA"
                     /locus_tag="ACHJZ2_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO4568024.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            1814..2155
                     /gene="ynfB"
                     /locus_tag="ACHJZ2_001903"
     CDS             1814..2155
                     /gene="ynfB"
                     /locus_tag="ACHJZ2_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO4568025.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2190..2750
                     /gene="speG"
                     /locus_tag="ACHJZ2_001904"
     CDS             2190..2750
                     /gene="speG"
                     /locus_tag="ACHJZ2_001904"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO4568026.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(2753..3463)
                     /gene="ynfC"
                     /locus_tag="ACHJZ2_001905"
     CDS             complement(2753..3463)
                     /gene="ynfC"
                     /locus_tag="ACHJZ2_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO4568027.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3571..3876
                     /gene="ynfD"
                     /locus_tag="ACHJZ2_001906"
     CDS             3571..3876
                     /gene="ynfD"
                     /locus_tag="ACHJZ2_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO4568028.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4075..6501
                     /gene="ynfE"
                     /locus_tag="ACHJZ2_001907"
     CDS             4075..6501
                     /gene="ynfE"
                     /locus_tag="ACHJZ2_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO4568029.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            6562..8985
                     /gene="ynfF"
                     /locus_tag="ACHJZ2_001908"
     CDS             6562..8985
                     /gene="ynfF"
                     /locus_tag="ACHJZ2_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO4568030.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            8996..9613
                     /gene="ynfG"
                     /locus_tag="ACHJZ2_001909"
     CDS             8996..9613
                     /gene="ynfG"
                     /locus_tag="ACHJZ2_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO4568031.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            9615..10469
                     /gene="ynfH"
                     /locus_tag="ACHJZ2_001910"
     CDS             9615..10469
                     /gene="ynfH"
                     /locus_tag="ACHJZ2_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO4568032.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            10512..11126
                     /gene="dmsD"
                     /locus_tag="ACHJZ2_001911"
     CDS             10512..11126
                     /gene="dmsD"
                     /locus_tag="ACHJZ2_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO4568033.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            11284..12576
                     /gene="clcB"
                     /locus_tag="ACHJZ2_001912"
     CDS             11284..12576
                     /gene="clcB"
                     /locus_tag="ACHJZ2_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO4568034.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(12529..13224)
                     /gene="bioD"
                     /locus_tag="ACHJZ2_001913"
     CDS             complement(12529..13224)
                     /gene="bioD"
                     /locus_tag="ACHJZ2_001913"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4568035.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(13349..14569)
                     /gene="mlc"
                     /locus_tag="ACHJZ2_001914"
                     /gene_synonym="dgsA"
     CDS             complement(13349..14569)
                     /gene="mlc"
                     /locus_tag="ACHJZ2_001914"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO4568036.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(14704..15597)
                     /gene="ynfL"
                     /locus_tag="ACHJZ2_001915"
     CDS             complement(14704..15597)
                     /gene="ynfL"
                     /locus_tag="ACHJZ2_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4568037.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            15704..16957
                     /gene="ynfM"
                     /locus_tag="ACHJZ2_001916"
     CDS             15704..16957
                     /gene="ynfM"
                     /locus_tag="ACHJZ2_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4568038.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(17006..17125)
                     /locus_tag="ACHJZ2_001917"
     CDS             complement(17006..17125)
                     /locus_tag="ACHJZ2_001917"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO4568039.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            17381..17689
                     /gene="asr"
                     /locus_tag="ACHJZ2_001918"
     CDS             17381..17689
                     /gene="asr"
                     /locus_tag="ACHJZ2_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO4568040.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            17782..17865
                     /locus_tag="ACHJZ2_001919"
     CDS             17782..17865
                     /locus_tag="ACHJZ2_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568041.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            17965..18786
                     /gene="ydgD"
                     /locus_tag="ACHJZ2_001920"
     CDS             17965..18786
                     /gene="ydgD"
                     /locus_tag="ACHJZ2_001920"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO4568042.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(18825..19154)
                     /gene="mdtI"
                     /locus_tag="ACHJZ2_001921"
     CDS             complement(18825..19154)
                     /gene="mdtI"
                     /locus_tag="ACHJZ2_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO4568043.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(19141..19506)
                     /gene="mdtJ"
                     /locus_tag="ACHJZ2_001922"
     CDS             complement(19141..19506)
                     /gene="mdtJ"
                     /locus_tag="ACHJZ2_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO4568044.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            19918..20952
                     /gene="tqsA"
                     /locus_tag="ACHJZ2_001923"
     CDS             19918..20952
                     /gene="tqsA"
                     /locus_tag="ACHJZ2_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO4568045.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLRIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(20977..22365)
                     /gene="pntB"
                     /locus_tag="ACHJZ2_001924"
     CDS             complement(20977..22365)
                     /gene="pntB"
                     /locus_tag="ACHJZ2_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO4568046.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(22376..23908)
                     /gene="pntA"
                     /locus_tag="ACHJZ2_001925"
     CDS             complement(22376..23908)
                     /gene="pntA"
                     /locus_tag="ACHJZ2_001925"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO4568047.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            24432..25376
                     /gene="ydgH"
                     /locus_tag="ACHJZ2_001926"
     CDS             24432..25376
                     /gene="ydgH"
                     /locus_tag="ACHJZ2_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO4568048.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            25562..26944
                     /gene="ydgI"
                     /locus_tag="ACHJZ2_001927"
     CDS             25562..26944
                     /gene="ydgI"
                     /locus_tag="ACHJZ2_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4568049.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            26981..27703
                     /gene="folM"
                     /locus_tag="ACHJZ2_001928"
     CDS             26981..27703
                     /gene="folM"
                     /locus_tag="ACHJZ2_001928"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO4568050.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(27700..28035)
                     /gene="ydgC"
                     /locus_tag="ACHJZ2_001929"
     CDS             complement(27700..28035)
                     /gene="ydgC"
                     /locus_tag="ACHJZ2_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO4568051.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            28164..28883
                     /gene="rstA"
                     /locus_tag="ACHJZ2_001930"
     CDS             28164..28883
                     /gene="rstA"
                     /locus_tag="ACHJZ2_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO4568052.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            28887..30188
                     /gene="rstB"
                     /locus_tag="ACHJZ2_001931"
     CDS             28887..30188
                     /gene="rstB"
                     /locus_tag="ACHJZ2_001931"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO4568053.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            30264..31193
                     /gene="tus"
                     /locus_tag="ACHJZ2_001932"
     CDS             30264..31193
                     /gene="tus"
                     /locus_tag="ACHJZ2_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO4568054.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(31190..32593)
                     /gene="fumC"
                     /locus_tag="ACHJZ2_001933"
     CDS             complement(31190..32593)
                     /gene="fumC"
                     /locus_tag="ACHJZ2_001933"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO4568055.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(32736..34382)
                     /gene="fumB"
                     /locus_tag="ACHJZ2_001934"
     CDS             complement(32736..34382)
                     /gene="fumB"
                     /locus_tag="ACHJZ2_001934"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4568056.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            34581..35756
                     /gene="manA"
                     /locus_tag="ACHJZ2_001935"
     CDS             34581..35756
                     /gene="manA"
                     /locus_tag="ACHJZ2_001935"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO4568057.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            35857..37365
                     /gene="ydgA"
                     /locus_tag="ACHJZ2_001936"
     CDS             35857..37365
                     /gene="ydgA"
                     /locus_tag="ACHJZ2_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO4568058.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVREAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(37410..38674)
                     /gene="uidC"
                     /locus_tag="ACHJZ2_001937"
                     /pseudo
     CDS             complement(37410..38674)
                     /gene="uidC"
                     /locus_tag="ACHJZ2_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
BASE COUNT         9718 a         9877 c         9409 g         9693 t
ORIGIN      
        1 tatctggcaa aaactgatcc cgagtcaggc tgattcttaa tgttcaactg tcgccggttg
       61 tgactggtat tctgcaaccg gcgctcgtct gatgtaagag ataaagaaat cgatgagcga
      121 agtaatcatg attgtctctc ccggcaaatg ggtatccgaa gagcagttaa ttgcgctgaa
      181 aggaataaaa aaaggtacgt taaaaaaggc ccgggaaaaa tcgtttatgg aaggaaggga
      241 atataagcat gtcgctcatg acggtatgcc atgggataac agtccatgct tttacaacct
      301 ggaagaaatt gatcgctgga ttgagcgcca ggcatcagca agaccaagac gtcatcttac
      361 ttgactaaaa gccacactaa ctaatgagag aagttgaaat gaaatatccg acaggcgtgg
      421 aaaaccatgg agggaaatta cgtatctggt ttgtttataa aggcgtaaga gtcagggaaa
      481 acctggggtt cctgacacag caaaaaacag gcgcgttgca ggtgagctac gcgcctctgt
      541 ttgttacgca ataaaaactg gtgttttcga ctatgcaaaa cagtttccct cctcacgcaa
      601 tctggaaaaa tttggtgagg cccgacaaga tttaaccata aaagaactgg ctgaaaaatt
      661 tctggcactg aaagaaactg aagtcgcaaa aacatcactc aacacatacc gtgccgtcat
      721 caaaaatatc ctgagcataa tcggtgaaaa aaatcttgcc tcatcgatta ataaagaaaa
      781 attactggag gttcgtaaag agctactgac tggataccag atccccaaaa gtaactatat
      841 tgttacacaa ccagggagat cggctgtaac tgtaaataat tacatgacaa atcttaacgc
      901 cgtgttccag tttggtgttg ataacggtta cctggcagat aacccgttta aggggatctc
      961 gccattaaag gaatcaagaa ccattccgga tcctctttcg cgggaggaat tcatccgtct
     1021 tatcgatgcg tgcagaaatc agcaagccaa aaatttatgg tgtaataccg tcctcagtgg
     1081 tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac aaaaacttca gcctttacga
     1141 tcttcatgtt cgattccttg catcgcttgt cgtgatgcat gaaatctacg caactgagct
     1201 actaccatat aagtatgaag atcgaaaaaa gccggagtga tcacaaaaaa aggcgtatat
     1261 ttgcgctgtg aatggttgac aaaagatgaa atagaatacc ttttgtcagc tgacacttcc
     1321 tcgtatctta ttgataaaat ggatttatgt tcctacgtgc gcccccagcc cacaacaatg
     1381 atcaacatgc cgcaaagcgc aatcaacggc cccgtccagt cataaagagt cagtttcacg
     1441 ccatccacaa cgcgcagcca catcaacgcc gtgcagacat aaacgccacc ataagccgcg
     1501 taaacacgcc cactcgccgc aggatgcaac gttaacaacc agacaaacag cgccagtgaa
     1561 atccccgccg gaagcaacag ccagatactg gcgtttcgtt ttaaccacaa ccagggcaga
     1621 aagcatccaa taatttcaca cagcgcagta gcaaaaaata gtaacgttgt tttaatcatc
     1681 tttgtcactt attgacatca tgtatagtta tagggcgaca taatatcatc aatataaaca
     1741 ccctcctggt acgttttgcg ttcgcagtgg atggtgtaga atcatcttta atcattcata
     1801 cagggaatga attatgaaaa tcactctcag caaacgaatc gacctgctcg cttttctgct
     1861 gccttgcgca ctggcattga gcacaactgt tcatgccgaa actaacaaac tggtgattga
     1921 gtctggcgac agtgcacaaa gccgccagca cgccgctatg gaaaaagagc aatggaatga
     1981 cacgggcaat ctgcgccaga aagtgaataa acgcactgaa aaagagtggg ataaagccga
     2041 cgccgctttt gataaccgcg ataaatgcga gcaaagcgcc aacatcaatg cctactggga
     2101 gccaaatact ttgcgctgcc tggaccgtcg aactggccgc gttattaccc cctaacctgt
     2161 tattgattta aggaatgtaa ggacacgtta tgccaagcgc ccacagtgtt aagctacgcc
     2221 cgctggagcg tgaagattta cgctatgtac atcaactcga caataacgcc agtgtgatgc
     2281 gttactggtt tgaggaaccc tacgaagcct ttgttgaact ctctgatctg tatgataagc
     2341 atattcacga tcagagcgaa cgacgctttg tggtggaatg tgacggcgaa aaagccggtc
     2401 tggtggagtt ggtggaaatt aaccatgtac atcgccgcgc agaatttcag ataattatct
     2461 ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc caaattagca atggactatg
     2521 gcttcaccgt tctcaatctc tataagctgt atctgatcgt tgataaagag aatgaaaaag
     2581 cgattcacat ttaccgcaag cttggctttt cggttgaagg tgaattgatg cacgagttct
     2641 ttattaatgg tcaatatcgt aatgccattc gcatgtgtat attccagcat cagtatctgg
     2701 cagagcacaa aacaccgggt cagactctcc tgaagccgac cgcacaatag cattaataat
     2761 aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc gggttttact ccttcatcaa
     2821 caatgatcag ctgacagccc accggattgg cgtgactgtc atattcacag ctctgtttta
     2881 cattaccaac tcgttgatta ttcagtaaag taaccgctgt gtaatctaat tttttgatcg
     2941 ggtccgttga tggcgtggcg ctgacagata atgttttgtc gttacttttc gtggttttac
     3001 ccagtggata accctgatca tcatagcgat attccatctg catttgtttg ctgctggctt
     3061 taatcacgaa gccattatca tcggtttccc aactcacccc ggcagaaggt aattctgcta
     3121 gctggcattt tccctgtaaa cgtactctct tctccagcgt ctcggcatca cggtaataat
     3181 tggcgtccag taccagagcg accacggtat tattttccag atccagtaat tcgagtgaat
     3241 caaaacagcc ttcttccgac aaagtccccg aaacacgttt cgtcacttca ccttgctcat
     3301 ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag cggatcaaaa tcgaattcat
     3361 tagagaaact ggccatctca ggagtaaatg aaagtgttac ctctgtgcgg tcacatcctg
     3421 tgagtaatat cgcgagtaag catggcagta atttgtattt cacatcagtc accagagagt
     3481 agagatgatt ctcaatcata gtagcaaata cagtacttta cacgtgaaat gctatgctta
     3541 aagaagttat cttcgcgtta ggagcttatg atgaaactct caacctgtta tgccgcgctt
     3601 ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg gatcctgtga gcgcatacaa
     3661 agcgatatat cacagcgcat tatcaataat ggcgtacccg aaagcagctt cacgttaagt
     3721 attgtaccca atgaccaagt tgatcagcct gattcccagg tcgtcgggca ttgcgctaat
     3781 gatacgcata aaattctcta cacccgcacc accagcggta acgtctctgc tcccgcgcag
     3841 tctacccagg atggcgcgcc tgccgaaccg cagtaataca ttatcgacta cacgccggat
     3901 acgacacatc cggcgatttg gacatacaac ataatcccac catattactc atacccttct
     3961 attgatatgg attaataatt attaacccaa aatgggtaga ctccctctat tgttagcgcg
     4021 ctaaatattc aatatataaa cttttatata acgataaaga acagggagtg agttatgtcc
     4081 aaaaatgaac gaatggtggg catcagccgc agaacactcg ttaaatctac cgcgataggt
     4141 tctctggcgc tggctgcagg cggtttttct ttgccgttca ccctgcgcag tgcagcggca
     4201 gcagtacaac aggccagcga acaagtggtc tggggtgcct gttccgtcaa ctgtggtagc
     4261 cgctgtgcac ttcgtctaca tgttaaagat aatgaagtgg cctgggtgga aactgacaac
     4321 accggcagcg atgagtatgg caaccatcag gtacgcgcct gtttgcgcgg tcgctccatc
     4381 cgccggcgta ttaatcatcc cgatcgcttg aattacccaa tgaaacgcgt gggtaaacgc
     4441 ggtgaaggca aattcgaacg gattagctgg gatgaagccc tggatactat cgccagtagc
     4501 ctgaagaaaa ccgtcgaaca atatggcaat gaggctgtat atattcagta ctcttcgggg
     4561 atcgttggcg gcaatataac ccgctcttcg ccatcagcct cggcggtcaa acgcctgatg
     4621 aactgctacg gcggttcact caaccagtat ggctcctaca gcactgcgca aatttcctgt
     4681 gccatgccct acacctacgg cagtaatgat ggcaacagca ccacggatat tgaaaacagc
     4741 aagctggtcg tgatgtttgg taacaacccg gcagaaaccc gcatgagcgg cggtggcatc
     4801 acttatcttc ttgaaaaatc ccgcgaaaaa tcgaacgcaa aaatgattgt tatcgatccg
     4861 cgatataccg atacggctgc tggtcgtgaa gatgaatggc ttcctattcg cccgggcacc
     4921 gatgccgcgc tggttgcggg tattgcctgg gtattgatta acgaaaatct cgttgatcaa
     4981 ccttttctcg ataaatactg cgtcggctat gacgaaaaaa ccttacccgc agatgcaccc
     5041 aaaaatggtc actataaagc ctatattctt ggtgaaggtg acgataaaac agcgaaaacg
     5101 ccgcagtggg cttcgcaaat taccggtatc ccggtggacc gtatcatcaa actggcgcgt
     5161 gaaattggca cagcaaaacc cgcttatatc tgccagggct gggggccgca acgccaggca
     5221 aacggcgagc tgaccgcacg cgctattgct atgttgccta ttctgacggg caatgtcggc
     5281 atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata ccattaccat agagcgcctg
     5341 ccggtgctgg ataatccggt caaaacgtca atttcctgct tcagctggac agatgctatc
     5401 gatcatggcc cacaaatgac ggcaatccgc gacggtgtcc ggggcaaaga taaactggat
     5461 gtgcccatta agttcatctg gaactacgcg ggaaataccc tggttaatca gcattctgac
     5521 atcaacaaaa cgcatgaaat tctgcaggac gaatcgaaat gcgaaatgat tgtggtcatc
     5581 gaaaacttta tgacctcatc ggcaaaatat gccgacattc tgctgccaga cctgatgacc
     5641 gttgagcagg aagatattat tcctaacgac tacgccggta acatgggata tctcattttc
     5701 ctccagcctg tcaccagcga aaaattcgaa cgcaaaccga tttactggat cctgagtgaa
     5761 gtcgcgaaac gtcttggacc tgacgtctat caaaagttca ctgaaggtcg cacgcaggaa
     5821 caatggttac aacatctgta cgccaaaatg cttgccaaag atccggcgtt accgtcttac
     5881 gacgaactga aaaaaatggg tatctataag cgtaaagatc ccaatggtca ttttgtcgcc
     5941 tacaaagcat ttcgtgacga ccccgaggca aatccactta aaacaccttc cggtaagatt
     6001 gaaatttatt ccagcaagct ggcggaaatt gcccgtacct gggaactgga aaaagatgaa
     6061 gtgataagcc cactgccagt ttatgcctca acctttgaag gctgggactc ccctgaacgt
     6121 agtacctttc cactgcaact gtttggtttc cattacaaat cccgtactca ctcgacctac
     6181 ggcaacattg atgtcctgaa ggctgcctgc cgtcaggagg tgtggatcaa ccctatagac
     6241 gcacagaaac gtgggattgc caacggcgat atggtgcggg tgtataacca tcgtggcgaa
     6301 gttcggctac cagccaaagt gacaccacgc attctccctg gggttagcgc tatgggccag
     6361 ggagcctggc acgaggccaa tatgtctggc gataaaatcg accatggcgg atgtgtgaat
     6421 acgctaacca ctctgcgccc ttcaccactg gcgaagggaa acccgcagca cacgaatctg
     6481 gtcgagatcg aaaaaatata acccactaca accataaatt ctggcatgac attttgtttg
     6541 aaaagcaata agtgagtaat gatgaaaatc cataccacag aggcgctcat gaaggctgaa
     6601 atcagccgta gaagtctgat gaaaacctcc gcacttggca gtcttgcgct ggcaagcagt
     6661 gctttcactc tgccattttc ccaaatggtc cgagcggcag aggcaccggt agaagagaaa
     6721 gcggtctgga gttcctgcac cgttaactgc gggagccgct gtctgttacg tttgcatgtg
     6781 aaagatgaca ccgtgtactg ggtggagtct gatacgacag gtgacgacgt ctacggtaat
     6841 catcaggttc gtgcgtgttt acgcgggcgc tctatccgcc gacggatgaa tcatcctgat
     6901 aggttgaaat atcccatgaa gcgcgtcggc aagcgcggtg aaggtaaatt tgaacggata
     6961 agttgggacg aagccctgga taccatcagt gataatcttc ggcggatcct gaaagattac
     7021 ggcaatgagg ctgtacatgt cctgtacgga acaggcgtag atggcggaaa catcaccaac
     7081 tcaaacgtcc cgtaccgtct gatgaactct tgcggtggtt ttctcagtcg ctatggcagc
     7141 tacagtaccg cacagatcag tgccgcaatg agttatatgt tcggtgccaa tgatggcaac
     7201 agcccggatg atatcgccaa tacgaaactg gtcgttatgt tcggaaataa cccggcagaa
     7261 acgcggatga gcggtggtgg tgtcacttac tacgtcgagc aagcccgcga acgttcaaac
     7321 gcacgcatga tcgtcatcga tccacgttat aacgacacag ctgccgggcg tgaagatgaa
     7381 tggctgccca ttcgccctgg caccgatggc gcactggcct gtgcgattgc ctgggtactg
     7441 attagtgaaa acatggtcga tcagccattt ctcgacaaat attgtgttgg ttacgatgaa
     7501 aaaacgctgc ccgccaacgc accacgtaac gcgcattata aagcctatat tctgggcgaa
     7561 gggcctgacg gcatagctaa aacgccggaa tgggcagcaa aaatcaccag catcccggca
     7621 gaaaaaatta tccagttggc acgagagatc ggttcagcaa aacctgctta tatttgtcag
     7681 ggttgggggc cacaacgaca ttccaacggc gagcaaacat cccgcgctat tgccatgctt
     7741 tccgttctca ccggtaacgt cggcataaac ggcggcaact caggtgtacg cgaaggtagc
     7801 tgggatctgg gggtagaatg gttcccgatg ctcgagaatc ctgttaaaac gcagatttcc
     7861 gtctttacat ggacagatgc catcgaccat ggtacggaaa tgaccgcgac ccgtgatggt
     7921 gttcgtggaa aagaaaaact ggatgtcccc atcaagtttt tatggtgcta cgccagtaac
     7981 acattgatca atcaacatgg cgacatcaat cacaccaatg aggtgcttca ggatgacagc
     8041 aagtgcgaga tgattgttgg cattgaccac ttcatgacgg cctcggctaa gtattgcgat
     8101 atcctgttgc ccgacctgat gccaacagag caagaagacc ttatctctca tgaatctgca
     8161 gggaatatgg gctatgtgat cctcgcccaa cccgcaacct cagcaaaatt tgaacgcaaa
     8221 cccatctact ggatgctgag tgaagtcgcc aaacgcttag gaccggacgt ttatcaaacc
     8281 tttactgaag gtcgcagtca gcatgaatgg atcaaatatc tccatgcgaa aacgaaggaa
     8341 cgtaaccctg agatgcccga ctacgaggag atgaaaacga ccgggatctt taagaaaaaa
     8401 tgcccggaag aacactacgt cgctttccgc gcattccgtg aagatccaca ggcaaacccg
     8461 ttgaaaacac cttcggggaa aatcgaaatt tattctgaac gactggcgaa gattgcagat
     8521 acctgggaat tgaaaaaaga tgaaattatt catccccttc ctgcgtatac cccaggtttt
     8581 gatggctggg acgatcccct gcggaaaacc tatccactgc agttaacggg cttccactac
     8641 aaagcgcgta cccactccag ctacggcaat attgatgtgt tacagcaggc ctgcccacaa
     8701 gaggtgtgga tcaaccccat tgatgctcag gctcgcggta tccgtcatgg cgataccgtg
     8761 cgggtattta acaataatgg agaaatgctg attgccgcaa aagtgactcc gcgtattctg
     8821 cctggcgtta ccgccatcgg gcaaggtgcg tggcttaaag cggatatgtt tggtgaccgg
     8881 gtcgatcacg gcggcagtat caatattctg acctctcacc gcccttcacc gctggcaaag
     8941 ggaaacccgt cgcacagcaa tcttgtccag atcgaaaagg tttaaggagt agcccatgac
     9001 cacacaatat ggatttttta tcgattccag ccgctgtact ggctgtaaaa cctgcgaact
     9061 ggcgtgcaaa gattttaaag atcttggccc ggaagtcagt ttccgccgta tttatgaata
     9121 tgctggcggc gactggcagg aagataatgg cgtctggcac cagaacgtgt tcgcttatta
     9181 tctctccatc tcctgtaacc attgcgatga ccccgcatgt acaaaagtct gccccagcgg
     9241 cgcaatgcat aagcgtgaag atggctttgt ggtggttgat gaagatgttt gtatcggctg
     9301 ccgctactgc cacatggctt gtccgtacgg cgcgccacag tacaatgctg aaaaagggca
     9361 catgacgaag tgcgatggtt gttattcgcg cgtcgccgag gggaaacaac ccatatgtgt
     9421 cgaatcctgc ccgctgcgtg cacttgagtt cggtccaatt gaagagctgc gccagaagca
     9481 cggcactctt gctgccgtcg cgccactgcc gcgcgcgcat ttcacaaaac ccaatatcgt
     9541 tatcaaacct aacgccaaca gccgcccgac cggcgatacc actggttatc tggctaatcc
     9601 ggaggaagtg taaaatggga aatggatggc atgaatggcc actggttatc tttaccgtat
     9661 tgggtcaatg cgtagtaggc gcgctgattg ttagcggaat tggctggttc gccgccaaaa
     9721 ctgacgcaga ccgtcagcac attgttcgtg gcatgttttt cctctggctg ttgatgggca
     9781 tcgggtttat tgcctctgtc atgcacctgg gatcgccact gcgggcgttt aactcgctga
     9841 atcgaatcgg agcttctggt ctgagtaacg agattgctgc cggttccata ttttttgccg
     9901 ttggcggact atggtggctg gtggcggtca tcggtaaaat gccgcaagcg ttggggaaac
     9961 tctggttact tgtcagcatg gcgctcggcg tcattttcgt ctggatgatg acctgcgtgt
    10021 atcaaatcga cactgtgcca acctggcata acggttacac tacgctggct ttcttcgtga
    10081 cggtattgtt gagtggtcca atacttgctg ccgcaattct gcgggcagcc cgcgttacat
    10141 ttaataccac accatttgcc atcattagcg ttctggcgct gatcgcttgc gccggggtga
    10201 ttgtgctgca aggtttgtct ctgtcgtcta ttcactcatc cgtgcaacaa gccagcgcac
    10261 tggtaccaga ttacgcctcc ttacaggtct ggcgtgtggt attgttatgc gcaggtctag
    10321 gatgctggct ttgtccgctc atacgtcgcc gtgaacctca cgttgccgga cttattctgg
    10381 ggttaatcct cattcttggc ggtgagatga tcgggcgtgt gctcttttat ggcctgcaca
    10441 tgacggtagg aatggctatt gcaggttaac aaattggtgc ggggcaaccc gcacatttca
    10501 ggatgttagg aatgacccat ttttcacagc aagataattt ttctgtcgcg gcgcgcgtgt
    10561 tgggcgcgct gttttattac gctccagaga gtgctgaagc cgcacctctg gttgcggtac
    10621 ttaccagtga cggctgggaa actcagtggc ctctaccaga agcgtcatta gcgccgctgg
    10681 tgacggcttt tcagacacag tgtgaagaga ctcacgccca ggcctggcag cgtttgtttg
    10741 tcggcccgtg ggcactgccg tctccgccgt gggggtcggt ctggctggat cgcgaatctg
    10801 tgctgtttgg cgattcaaca ttggcacttc gtcagtggat gcgcgagaaa ggcattcagt
    10861 ttgaaatgaa gcaaaacgaa ccggaagatc attttggttc actgctgttg atggctgcgt
    10921 ggttggcgga gaatggtcgc cagacagaat gcgaagaatt gctggcatgg cacctttttc
    10981 cctggtcaac acgttttctt gacgttttta tcgaaaaagc agaacaccct ttctaccgtg
    11041 cactgggtga actggctcgc ttaacgctgg cgcaatggca atcacaactg ttaattcctg
    11101 tcgcggttaa accactcttt cgataggatc accgtaatat tgccggatgg tgacgattac
    11161 gcgcgattcg gcaacaatac gttacagtgt cacgtttttt gtctcttaaa gcacgcactg
    11221 cttttgcagc tggcctcttt tgccgcaaaa tagtcgcccg tgtttcattg cccatttctg
    11281 ctcatgcatc atctacacac ctatccggat ctgcgcacta tgttccgccg tctgcttatc
    11341 gcaacagtcg tcggtattct cgcggccttt gccgttgccg ggtttcgtca tgcgatgctg
    11401 ctactggagt ggttgttcct caataatgac tccggcagtc tggtcaatgc agcgacaaac
    11461 ctttccccct ggcgacggct gctaactccg gcgctcggcg gactggcggc gggtttgttg
    11521 ttgatgggct ggcagaaatt tacccagcaa cgccctcatg cgccgaccga ttacatggaa
    11581 gcgttgcaaa ccgatggtca gtttgattac gcagcaagcc tggttaaatc gcttgcctct
    11641 ctgctggtag taaccagcgg cagtgcaatt ggtcgcgaag gtgcgatgat tcttttagct
    11701 gcccttgccg cctcctgttt tgcccaacgt tttacgccac gccaggagtg gaaattatgg
    11761 atcgcctgtg gggccgcggc gggaatggct gcggcctatc gtgccccgct tgctggcagt
    11821 ttatttatag ccgaagtgct gtttggcact atgatgttgg cctctctcgg cccggtgatt
    11881 atttccgccg tcgtggcatt gctggttagc aatctgatta atcatagcga cgcgttactc
    11941 tacagcgtac aactctcagt gacggttcag gctcgtgact atgcgctgat tatcagtaca
    12001 ggtgtgctgg caggtctgtg cggaccactg ttgttaacgt taatgaacgc ctgtcatcgt
    12061 ggatttgtga gtctcaaact tgcgccgccc tggcaactgg cactaggcgg gttgatcgtg
    12121 ggtctgcttt ccctgttcac acctgcagtg tggggcaacg gctatagcac cgtacaatcc
    12181 tttttaaccg ccccgccact gttaatgatc attgccggga tcttcctttg taaactgtgt
    12241 gccgtgctgg cgagtagcgg ttccggcgca cccggtggtg tctttacacc gacgctattt
    12301 atcggtcttg ccattggcat gttgtatggt cgtagtctgg gattatggtt ccctgatggc
    12361 gaagaaatta cgcttttact cggattgacc gggatggcga cactgttggc ggcaaccacg
    12421 cacgcgccga ttatgtcgac gttgatgata tgtgaaatga ccggggagta tcagctactc
    12481 cccggtttat tgattgcctg cgtaattgcg tcggtaatct cgcggacgtt acaccgtgac
    12541 tctatctacc gccagcacac tgcgcagcat agctaagcgg atgtattgcc ccagttcacg
    12601 ctgttcggcg cgcggcagat aaggcagttc accaatgagc ggtgccggaa gttttttacc
    12661 gagcacatca atgatttccg cataatgcgc cagtcctggg ttgattcggt tagccaccca
    12721 gccaatgagc ggcagcccgt cgttggcaat cgcctgagct gttagcagtg catggttaat
    12781 gcaaccttcc tgaataccga caaccatcaa caccggcagt tgttcctgca ccacccattc
    12841 agagagtgga cgcaaatcat tcatcagact acgccagccg ccagtccctt ctaccacgac
    12901 atgatcgact ttttcggtca ggtttgccag gccgtttgaa atgagggtgt aattgattgg
    12961 gcaactgtgc gccacgctac tttcttcttc gcttaacgcg ataggattaa ctgcttcata
    13021 aggcagttcg atggttgaaa cactctgcaa caccagggca tctttattac gcagcccttc
    13081 gggtgtctct ttgctcccct tcgctacggg tttatatccc gcaaccgttt ttccctggga
    13141 ggctaacgct tgtagcaatg cgcgggaaac caccgttttc cctacagaag tgtctgtacc
    13201 ggtaataaag aaacgcttca gcatcactaa ctccaccgtt atgcttcaca aatataaact
    13261 aggaaaataa ttaaccttga aagtctaagt tatgctttcc tggcccaaat tgagatagcg
    13321 caaattttgg tagaacagtt aaaaaatgtt aaccctgcaa cagacgaatc aacaaagaac
    13381 cgttatacat cgcgtctttt accagcgcag cccctgccat cgtgccctgg ttagaaaact
    13441 gagtactctc aacgctaatg tgctgactat acgcaggaag ggcctgctga cggatgctat
    13501 ctgagatgac cgggaagagg atatctgccg ctttacttaa cggtgagcca atcagtattt
    13561 tttgtgggtt aaataaattc accatgatgg caagaatgcg ccctacatgc gcgcccaccc
    13621 cggtaatgat gtcttttgcc agcagatcgc cgcgcaatgc cgcctgacac aatgagtcca
    13681 cggttaacgg ttgcccatgt aacatcgagc tcatggattg attgagacgc agctgtgcca
    13741 gctcaagaat actgtccaca ctggcgatgg tttcgaggca gccgtggttc ccgcaataac
    13801 agcgtttacc atatgggtct acctgtgtgt gacctatttc cacgaggcta ctgctgcctg
    13861 cgtgtagcag atgaccatcg gtaatgacgc ccgcccccac gttgtgatcg ataaccacct
    13921 gaatcacatc gcgcgccccg cgtgaggcac caaacaaggc ctctgccatc gtccatgcgc
    13981 tgatatcatg ctgaatataa accggaacgc cggtatgctg ctccagagcc tcgccgagcg
    14041 gcatctcttt tacatcctcg tagaacggca tgcgatgtac aatgccattt tccgtatcaa
    14101 taattcccgg cagggttatg gcaatcgaag ttagacgctc aagttttttc tggtggcgga
    14161 taaaaaactg atcgatatgg gaaatgatac gatccagcaa tggcgagtca tcttttaacg
    14221 ccagttcctg cgactcttcc accaccagtt tgctgctcag atcgcgcaga gcaaggaaaa
    14281 tctccccgcg actaatgcgc agagaaagat agtgccaggc ttcagtttca accaccagcc
    14341 ccaccgccgg acggccacgg ttccccgctt ctttgatttc cagctcttgc accaggtgtg
    14401 cttcgagcat ctcacggaca attttagtga tactggcagg agccagctgc gccagacggg
    14461 aaagatcgat acgcgagact ggaccaagct gatcaatcag gcgataaacc gcgcccgcgt
    14521 tggtctgctt tatttgatca atgtgcccag gctggttttc agcaaccacc gcatactccc
    14581 tatattttcg cgctccgaaa taatctgtag gctatggtga agcacttcaa tacgtgtcgt
    14641 caaattttta cttaggcatg tgattaacag cacatttttc gggctttttc gctgaaattt
    14701 ccctcacctg agagcattca gcagatgaat acgaaagtta cgcgcagctg gactttgttc
    14761 atgatgtttc ggccagacca accacatttc agaaaccgca tcctcttcag caatcggcac
    14821 ccagcgcatt tcgttgagct gaacacgttt aaatgacgca ggcaaaattg aaacacccag
    14881 accggcggaa accagaccga tgatggtcat tgcctcgccc acctcctgag tgatgacggg
    14941 cgtcaaatgg taacgtcgca tcagcccgag aatatcgtca tacagccctg tcccgacgtg
    15001 cggatcaaaa aagacaaagg gttctttcgc cagttcagcc agcgttacat tcgggttatt
    15061 tgccagggga tgatcgtgcg ggatcatcgc cataagcggt tcatggacga tgactgcgtg
    15121 ttcaagcgtc tccggtaacg ctgtgttacg cagcaatccc atatccagcg ttccttcaat
    15181 gagcggagcg atttgctcgc gagtgttcat ttcgcgggtt tgtaaatgga catcgggata
    15241 atctcggcga aacagcgata acgtatcgga caccgcccga ataaaaggag ccgacgaagt
    15301 aaaaccaatg cgcaactccc ccgcttcacc ctgatgcagt ctttcagcgc gagcggcagc
    15361 gtcatccacc atagacagga tttgccgact atctgcaaga aactgttttc ctgctgccgt
    15421 cagcaataca ctgcgattgg ttcgtgccag cagtcgggca ccaatttgtt gctccagcgc
    15481 ctgaatctgc tgacttagcg gcggttgcga aatattcagg cgggcagcgg cgcgcccgaa
    15541 atgcagctct tccgcaacag caacaaagta acgcagatga cgaagttcaa tattcatatt
    15601 taaaacatct tatttgagat tattaatata ttagacagaa caattcgatt ttcctaacct
    15661 atgtataagc ctgatccaca ggcatattta gcaaggattt caagtgagcc gtactacaac
    15721 tgttgatggc gctccggcaa gcgacactga caagcaaagc atttctcagc caaatcaatt
    15781 tattaaacgc ggtacgccgc aatttatgcg cgtcaccctg gcgctgttct ctgccggact
    15841 ggcaacattt gcacttctct attgtgtgca acctatcctt ccggtgcttt cgcaggagtt
    15901 tggcttaacg cccgcgaaca gtagtatttc actgtccatt tccacggcga tgttggctat
    15961 tggtttgctg tttactggcc ccctatccga tgccattggt cgcaaaccag tgatggtaac
    16021 ggcgctacta ttggcctcca tttgtacgtt actttcgaca atgatgacca gctggcacgg
    16081 cattttgatt atgcgcgcct tgattgggct ttcgttaagt ggcgtggcag ctgttggcat
    16141 gacttatctt agcgaggaaa tccaccccag tttcgtggcc ttttcgatgg ggttgtatat
    16201 cagcggcaac tcaattggcg gcatgagcgg acgcttaatt agtggtgtct tcacggactt
    16261 tttcaactgg cgaattgctc tggcggctat cggttgtttc gcgctagcct cggcgttgat
    16321 gttctggaaa atcctcccgg aatcacgcca ttttcgcccg acttcgctgc gacctaagtc
    16381 gttgtttatc aactttcgtc tgcactggcg tgaccgggga ttaccgttat tgttcgcaga
    16441 aggctttttg ctgatggggt cgttcgtcac gctgtttaat tacatcggct atcggttgat
    16501 gctctccccc tggcatgtca gtcaggccgt ggttggctta ttatcgctgg cttatttgac
    16561 cggtacatgg agctcaccca aagccggaac catgaccacc cgctatgggc gtggtccagt
    16621 gatgttgttt tcgacggggg ttatgctgtt tggtttactg atgaccttat tcagctcgct
    16681 gtggctgatc tttgccggaa tgttactctt ctcagcagga ttcttcgcag cccactcagt
    16741 tgccagcagc tggatcggcc cccgcgcaaa acgcgctaaa ggccaggcct cctcgctgta
    16801 tctgttcagt tactatctgg ggtcgagtat tgccgggacg ctgggtggtg ttttctggca
    16861 taactatggc tggaacggcg tcggcgcatt tattgctctg atgctggtca ttgctctgct
    16921 ggtcgggacg cgtttgcatc gtcgtctgca cgcctgaaaa ataagtccgg actgcggtaa
    16981 atacccgtcc ggacttattg cctgctcaaa ccaacgttaa tagccatcct aaaatagacg
    17041 aagcgccagc caattcccgc agcgcgtcta gcgtcatcag gattataagt acccaaataa
    17101 acggattcat tttgctgtgt gtcatttatt actgatgcgc agttattcta ccgctttgta
    17161 agtagtaaaa tagttaaccc gatcaagact actattattg gtagctaaat tttccttaag
    17221 tcacaatacg ttattatcaa cgctgtaatt tattcagcgt ttgtacatat cgttacacgc
    17281 tgaaaccaac cactcacgga agtctgccat tcccagggat atagttcttt caacggcccc
    17341 gcagtggggt taaatgaaaa aacaaattga gggtatgaca atgaaaaaag tattagctct
    17401 ggttgttgcc gctgctatgg gtctgtcttc tgccgccttt gctgcagaga ctgcgaccac
    17461 acctgctccg actgcgacga ccaccaaagc agcgccggcg aaaactacac atcataaaaa
    17521 acagcataaa gcagcacctg cccagaaagc gcaggcggct aaaaagcatc ataaaaatac
    17581 gaaagctgaa cagaaagccc cagaacaaaa agcgcaggca gcgaagaaac acgccaagaa
    17641 acacagccat cagcaaccgg caaaacctgc tgcacaaccc gcagcgtaag ttttcaacag
    17701 taatgctggc gcgccccctc gcgcctgaaa attacggtgc taagcgggta acgtttagca
    17761 ccgcctttag cggagggtag tatggtgggc cgttatcgct ttgagttcat tcttatcatc
    17821 cttattttat gcgcactgat tgccgcccgt ttttatcttt cctgatcgta gttatctgat
    17881 tttactccca ctttcatccc gtcccgtcta tagtatttat gagggtttgc ttttaataat
    17941 cataattacc caccagagtg tgatatgcgt acaaccattg ctgtagtgtt gggtgcaatt
    18001 agtttgacgt ctgcttttgt gtttgcagat aaaccagacg ttgccaaatc ggcaaacgat
    18061 gaggtcagca ccctgttttt tggtcatgat gatcgtgtgc cagtgaatga cacgacccaa
    18121 tcaccgtggg atgcggttgg gcaactggaa acggccagcg gcaatttatg tacggcgacg
    18181 ctgattgcac ccaatctggc attaacggca ggacactgtt tattgacgcc accaaagggt
    18241 aaagccgata aagcagtggc gctgcgtttt gtgtcaaata aaggtctttg gcgctatgac
    18301 atccacgaca tagaaggccg cgttgatccg acactgggaa agcggttaaa agcagatggg
    18361 gatggttgga ttgtacctcc cgcagccgcg ccgtgggact tcggattgat tgtgctacgt
    18421 aatccccctt ctggcattac gccgttgccg ttatttgagg gagataaagc cgcgcttacc
    18481 gccgcattaa aagcggcagg tcgtaaagtg actcaggcag gataccctga agatcatctc
    18541 gatacgttgt acagtcatca aaactgtgaa gtgactggct gggcgcaaac gtcggtgatg
    18601 tcacatcagt gcgatacctt gccgggtgac agcggttcgc ctctgatgtt gcataccgat
    18661 gacggctggc aattaattgg ggtgcaaagt tcggctcctg ccgcgaaaga tcgctggcgc
    18721 gccgataacc gggccatttc tgttaccggt tttcgcgaca agctggatca actgtcgcaa
    18781 aaataatgtt caaacgctgc ccgacagcgc gggcagcgtc ttcatcaggc aagtttcacc
    18841 atgatcattc cagccaacag caagaccagg ccaatccagc ctttacgatt taaccgttga
    18901 ccaaacaaga tccaacctgc ggctaacgtg gcggcaatac caaacccgcc ccacaatgca
    18961 taagcgacag acaagtcgat ccctttaacg gcctgagaaa gcgcactaaa ggcagccagc
    19021 accgccgcca gggagagcaa gccaaatatt ttgcgacgaa agccgtcaga aaatttcaaa
    19081 aagacgttag caacgatttc cagcacgatt gccaatgcca gccaggcggc gtgaacccat
    19141 tcaaactgcg ccatggttca cctccagttc aggtttacgt gctttacggg tacctgattt
    19201 gatcaacact atcccggcga ccagggtggt taacccggca attttcatca gcgataaact
    19261 ttcgtcgaat aacaaaacgc taaacaaggt aataaataaa ataccgatac cttcccacag
    19321 cgcataagcc acacctaagg ctattttttt aacagcgaaa gagagaaata tatacgacag
    19381 agaaatcatc accagcatta aaataaagcc gccatttccc tcactgacgc tcgcccattt
    19441 catagacagc gtaccggtaa tttctgtagc aatagccaga cctaataaaa tccaataaat
    19501 atacattgtc cttctcctgc aagagaatta ttttaatttt cgcttaattc agcgaaacca
    19561 aagtaaagtg gtcaagcccc gcgtataggg caagcttagg cagaagaaag gactaaagcg
    19621 cgttgcgcca gtgctgctca cctacgagca agatagtgga tgaggtacga agatgggtaa
    19681 atgtagaaaa taacgtcctg aacaaattgt ccataatatt acaattatcc gcagtgttgc
    19741 ttctcgtcat cgcggatgat aattgtcctc ggtagttgaa cacgcctgat ttgtatcata
    19801 gcttaagaat taactcaaaa tattttcact tctttacctg agcggtttga ttttcgttat
    19861 gatgacggag cgaaaaagac attattatta gcaaaggaag aaaaaacggg gacaagcatg
    19921 gcaaagccgc tcatcacgct caatggccta aaaatcgtca ttatgttggg aatgctggtc
    19981 attattctct gcggtatccg ttttgccgcc gagatcatcg tgccgtttat tctcgcatta
    20041 tttattgctg ttattcttaa cccgctggtg caacacatgg tccgctggcg tgtgccgcgt
    20101 gtactggcag tgtcgatttt gatgaccatc atcgtgatgg ctatggtgtt gctgttagct
    20161 tatctgggtt ccacgctcaa cgagttgacg cggacgttac cgcaatatcg caactctatt
    20221 atgacgccgc tgcaagctct tgagccgttg ttgcaacgcg tagggattga cgtctcagtt
    20281 gaccaactgg cgcattacat tgatccgaac gcggcgatga cgttgctcac caacttattg
    20341 acgcagttat ctaatgccat gtcatcaata tttttattgc tgctgacggt gctgtttatg
    20401 ctgctcgaag tgccacaatt gcccggaaaa tttaagcaaa tgatggcgcg tccggttgaa
    20461 gggatggcgg cgattcagcg tgcgattgac agtgtgtctc attatctggt gctgaaaaca
    20521 gccatcagca tcatcaccgg cctggtcgca tgggcgatgc tcgccgccct cgatgttcgc
    20581 ttcgcttttg tctggggatt gctggccttt gcgcttaatt acatccctaa tattggttca
    20641 gttctcgcgg caatcccccc tattgctcag gtactggtgt ttaatggctt ctacgaagcg
    20701 ttgttggtgc tggcgggata tctgctgatt aatctggtct tcggcaatat tctggagcca
    20761 cgcatcatgg ggcgtgggct gggactttct acattggtgg tatttttgtc gttgattttt
    20821 tggggatggt tgttaggacc ggtgggtatg ctgctttccg tgccgttaag aattattgtc
    20881 aaaattgcgc ttgaacaaac agcgggaggt caaagcatcg ccgttctgtt aagcgatctc
    20941 aataaagagt gacggcctcc gcagaggccg tcagggttac agagctttca ggattgcatc
    21001 cacgctggct ttggcgtcac caaacagcat gtgggtgttt tccttgaaga acagcgggtt
    21061 ttgcacacca gcatagccag tgttcatcga acgtttaaag acaatcacgt tctgcgcttt
    21121 ccacacttcc agcacaggca taccagcaat cggactcttc ggatcatcct gcgccgccgg
    21181 gttaaccgta tcgttagcac caatcaccag tacggtatcg gtatcagcaa agtcatcgtt
    21241 gatctcgtcc atttccagca cgatgtcata cggtactttt gcttcagcca gcaatacgtt
    21301 catatgtcca ggcaaacgcc ccgcaaccgg gtggataccg aaacgcacgt tgataccacg
    21361 agcgcgcagt ttctcggtaa tttcagcgac aggatattgc gcctgcgcga ctgccatgcc
    21421 gtaccccgga gtaatgatca ctgaatggga gtttttcaac agttccgctg tctcttctgc
    21481 ggtgatttcg cggtgctcac ccacttcctg atcatcgcca gtagaagagc cgtcggtgcc
    21541 gaaaccaccc gcaataacgc tgataaagga acggttcatc gccttacaca taatgtaaga
    21601 aaggatagcc cccgaggaac cgaccagcgc accggtcaca atcagcaggt cgttgctgag
    21661 cataaagccc gcagccgcag ccgcccagcc ggagtacgag ttcagcatcg acaccaccac
    21721 cggcatatct gcaccaccga tggaggcgac taaatgccag ccaaatacca gcgcaatggc
    21781 ggtcattatt agcaatgcca gcacttgcag gccgacgcta tccgtgcgaa caaatacaat
    21841 cagcagcagg aaggaaacga ccagagctgc caggttcatt ttgtgacggt ttggcagcat
    21901 caacggttta gacgaaattt tgccacacag tttgccgaac gccaccaccg aacccgtgaa
    21961 cgttaccgcc ccgatgaaga taccgagaaa cacttccgtc aggtgaatat tgaccagaat
    22021 cggtgccatt cccgcgtcat gatgcagata gctgttaaag ccaaccagca ctgccgccag
    22081 acccacgaag ctatgcagga tcgccaccag ttctggcatt tcggtcattt caactttctt
    22141 cgccagacgg ataccaattg ccccaccaat gaccatcgcc agcaagatcc agccaacgtt
    22201 acccgtatcc ggtccaaaaa tggtggcgat tagcgcaatc gccatcccgg cgataccgaa
    22261 gttgttaccc tggcgagacg tttcatgttt cgaaagcccg gccagactga agataaacag
    22321 gatcgcggca acaatgtatg cagctgtaac taatcctcca gacatatgtt accccttaat
    22381 ttttgcggaa cattttcagc atgcgctgag tcacggtgaa gccaccgaaa atattaatgc
    22441 tggctataag caccgcgata aaactaagga agctaaccca gccgccctgg ccaatctgca
    22501 acagtgctcc gacaacaata atccctgaaa tcgcgttggt gaccgacatc aacggtgtat
    22561 gcagcgcgtg cgatacgttc cacaccacgt aataaccgac aacgcaggcc agcgcgaaaa
    22621 cggtgaagtg cccaagaaac tctttcggcg caacgcttgc cagccagccg aaaaggatga
    22681 ttgccagcgc catcaacgcg tatttacgcc acggtgaaca ggcacatttt tcctcagttt
    22741 tcacttccgg tgccgctttt tgtgccacct gcggctgagc tgatacctga atcggcggtg
    22801 ccggccaggt aatttcgccc gcacggatca cagtcacgcc gcgaatcacc acatcatcaa
    22861 aatcaacagt gatgttaccg tttttctctt tgcacaacag tttcagcaga ttaacgaggt
    22921 tagtgccgta aagctgtgag gattgcgtcg gcagacggcc cggaagatcg gtatagccaa
    22981 tcactttgac accattttcc gtagtgaaga tttcacccgg cacggtgtat tcacagttgc
    23041 cgccgttttg ggctgccagg tcgacaatca cactgcccgc cttcatggag tcaaccattt
    23101 cacgggtaat tagcttcggc gctggtttgc ctggaataag cgcggtggtg acaatgatat
    23161 cgacctcttt tgcctgggcg gcaaagagtt ccatttccgc tttgatgaac gcgtccgaca
    23221 tcactttggc atagccatcg ccgctgcccg cttcctcttt aaaatccagc tcgaggaatt
    23281 ccgcgcccat actttgaact tgttctttca cttccgggcg ggtgtcgaat gcacgcacaa
    23341 tcgcgccgag actgtttgct gcgccaatgg cggccagacc tgcaacaccc gcaccaatca
    23401 ccatcacttt tgccggtggc actttcccgg ccgcagtaat ttgcccggta aagaagcacc
    23461 caaattcatg tgccgcttca acaatggcgc gataaccggc gatattcgcc atcgagctta
    23521 gtgcgtccag cgattgtgcg cgtgagatac gcggcacaga gtccatcgcc atcacggtca
    23581 cgttacgttc cgcaagtttt tgcattaatt ccggattctg cgcaggccag ataaaactca
    23641 ccagcgttgt cccaggattc agtaacgcaa tttcatcatc taacggcgca ttgaccttca
    23701 gaatgatctc tgactgccag acgctattcc cttctacaat ttcagcgccc gcttgcacaa
    23761 acgctttatc gtcaaaactt gccagttgac ccgcgccgct ctctaccgcg acggtaaacc
    23821 ccagtttcag cagctgttcc actgtttttg gcgttgctgc aacacggggt tcattggtta
    23881 accgttctct tggtatgcca attcgcatga tattcccttc catcggtttt attgatgatg
    23941 gtttgcctgt gtcaggagcc acacaagccg ctcatgtacg agctaaatgt tactccgtta
    24001 aaataaatta gtaacaaacg ccctataacg tactgaaaat tatgcctgtg atctagcgcc
    24061 aaaaatcagt atttcggcgt gaactcgcaa aatattaacg attcagccgt gatagtggga
    24121 taaacacctt agaacgccgg ataaagactg ataattgtct tcgacggtcg ggtaaaacga
    24181 gacatcgccc cggcacgaat cactacttaa cattaaatta acttatacaa ttcagttgct
    24241 tcagtagtaa tgatgctgat acggctgttt tttaagcata gacggtcatt tgagcaggat
    24301 taaaattggc ttaaggaatg tgatatgaaa aatgacgcag acagttacac cgtttaaatg
    24361 caataatcag ccacgtttct cgttaataac aataccagta cctggtttgc gcaaggcgaa
    24421 ggattatttt tatgaagctt aagaacaccc tcctggcgtc ggcactgctt tctgctacgg
    24481 cattctccgt taacgcagca acagaactga caccggagca agcggcagcg gttaaacctt
    24541 ttgaccgtgt agtggttacc ggtcgtttta atgctattgg cgaagcggtg aaagccgttt
    24601 ctcgtcgcgc agataaagaa ggtgccgcct ctttttatgt tgtcgacact tctgattttg
    24661 ggaacagcgg taactggcgt gtggtcgctg acctctataa agccgatgct gaaaaagcag
    24721 aagaaacaag taatcgcgta attaacggtg ttgtcgaact gccgaaagat caggctgttc
    24781 tgattgaacc gtttgacacg gtcaccgtcc agggcttcta tcgtagccag ccagaagtca
    24841 atgatgccat caccaaagcg gcaaaagcga aaggtgccta ctctttctac atcgttcgtc
    24901 aaatcgatgc caaccagggc ggcaaccagc gtattactgc attcatctat aaaaaagatg
    24961 ctaagaaacg tatcgtccag agcccggatg tgatcccggc agattccgaa gcaggacgtg
    25021 cagctctggc tgccggtggc gaagccgcga agaaagttga gatcccgggt gttgcgacta
    25081 ccgcatcacc aagttctgaa gtcggtcgct tctttgaaac ccagtcatca aaaggcgggc
    25141 gttacactgt cacgcttccg gatggcacta aagtcgaaga actgaacaaa gcgacagcag
    25201 cgatgatggt cccgttcgac agcatcaaat tctctggcaa ctacggcaac atgaccgaag
    25261 tctcctatca ggttgcgaaa cgagccgcga agaaaggtgc taaatattac cacatcaccc
    25321 gccagtggca ggaacgtggt aataacctga ccgtcagtgc agatctgtat aaataactat
    25381 tcagttactc ttataaggcg gcttgatgcc gcctttcgca tttttagtca caaatttccc
    25441 cgcgaccatt gcatgcagac acatctcttc gtaaaatccc gcgcttcagt ggcaaactcc
    25501 atttttatgc gttttgccct aatatttatt cttcttatca cgttttaatc actggatatc
    25561 gatggaaaag aaactgggac tgagcgcact caccgcgctg gtattaagct caatgctggg
    25621 cgcgggtgtt ttcagtctgc cgcaaaatat ggcggcagtt gccagcccgg cagcactgct
    25681 catcggctgg ggtattactg gcgctggcat tttattgctg gcctttgcca tgctgatcct
    25741 cacgcgcatt cgtcccgaac ttgacggcgg tatctttacc tatgcccgcg aagggtttgg
    25801 cgagctaatc ggcttttgtt ccgcatgggg atactggctg tgcgcagtca tcgccaacgt
    25861 ctcctatctg gtgatcgttt tttccgcgtt aagctttttt acggacacgc cggaattgcg
    25921 cctgtttggc gatggtaata cctggcaatc gatagtcggt gcatcggctt tattgtggat
    25981 tgttcacttt ttgatcctgc gcggggtgca aaccgctgcc agcattaacc tggtggcgac
    26041 actggcaaaa ttgttgccgc tgggtctgtt tgttgtgctg gcgatgatga tgttcaaact
    26101 ggatatcttc aagctcgact tcaccggact tgcacttggc gtacctgttt gggaacaggt
    26161 gaaaaacacc atgctgatca ccctgtgggt tttcattggt gtggaaggtg ccgtggttgt
    26221 ttctgcgcgt gcgcgtaata aacgtgatgt tggcaaagcg acactgctgg cggttctctc
    26281 cgctctgggc gtttacttgc tggtaacgct gctttcgctg ggagtggtgg cacgtcctga
    26341 actggctgaa attcgtaacc cgtcaatggc cggtctgatg gtggaaatga tgggaccgtg
    26401 gggcgaaatc atcatcgctg ccggtttgat cgtttccgtt tgcggcgcgt acttaagctg
    26461 gaccatcatg gcggcagaag ttccgttcct ggctgccact cataaagcat tcccacgcat
    26521 ttttgcgcgc cagaacgcac aagctgcgcc atccgcctca ctgtggttga caaacatctg
    26581 cgtgcaaatt tgtctggtac tcatctggct gaccggttcc gattacaaca cgttgctgac
    26641 catcgcctca gaaatgattc tggtgcccta tttcctggtc ggcgcgttct tgctgaaaat
    26701 cgcaacccgt ccactgcata aagcggtagg tgtcggtgcc tgcatttatg gcttatggtt
    26761 attgtacgcc tctggtccaa tgcacttgct gttatccgtt gttctgtatg caccggggct
    26821 gctggttttc ctgtatgcgc gcaaaacgca tacacatgat aatgtactga accgtcagga
    26881 aatggtgctg attggcatgc tgttaattgc ctcagttccg gcgacctgga tgctggtggg
    26941 ataacgttca tcccatcgtt ggatagcaaa ggagataacg atgggtaaaa cccagccctt
    27001 gccaatatta attactggcg gaggtcgtcg catcggcctc gccctcgcat ggcatttcat
    27061 taatcaaaag caaccggtga ttgtcagcta tcggacacac tatccagcca ttgatggact
    27121 gattaatgca ggtgcgcagt gtattcaggc tgatttttcg accaacgacg gtgtgatggc
    27181 gtttgccgat gaagtactaa aaagcaccca tggtctgcgt gctattttgc ataacgccag
    27241 tgcgtggatg gcggaaaaac cgggtgcgcc actggccgac gtactggctt gcatgatgca
    27301 gatccacgtt aataccccat acctgctcaa ccatgcgctg gaaagattac tgcgtgggca
    27361 cggacacgcc gccagcgata tcattcactt taccgattat gtggtggagc gcggtagcga
    27421 caaacatatt gcgtatgctg caagcaaagc ggcactggat aatatgaccc gctcgtttgc
    27481 ccgcaagctg gcaccggaag tgaaagtgaa ttctattgcg ccatcgctga tcctgtttaa
    27541 tgaacatgat gatgccgaat atcgacaaca ggcgctgaat aaatcattga tgaaaaccgc
    27601 gcctggcgag aaagaagtga tcgacctggt cgattactta cttaccagtt gctttgtcac
    27661 cggacgcagt ttcccacttg atggcggtcg tcatctgcgt taatgcagct ttatccagca
    27721 tataatcaac acccatgcgc ttatccccca acacgcaaca gaaccaacaa aagcggcggg
    27781 caagcgcatc attccggtga aataccacag cgacaccagg taaacaaaat agggaataat
    27841 cgaccacata ctaaaaatga tggttgcgcg taaggcttca atgccgcgtt cgctggcaac
    27901 aatataatgc gcgataagcg caaaggtcgg gaaaagtgga atcagcccgg cgatataata
    27961 atttttcgtt tttgctaaaa caccaatcaa cagcactacc agcgcaccga gcgcggcttt
    28021 gattaccagc cccatctttt taccttaaca cttccataac aagtcatcag tagaatacct
    28081 gatgaaaact tgtttagaaa cgattgatag taagtaaaaa cagcgcggtg tattgtgacg
    28141 tttttatatc taccgtgaat gttatgaaca ctatcgtatt tgtggaagat gatgcggaag
    28201 tcggttcact gattgccgcg tacctggcaa aacatgatat acaggttacc gtagagccgc
    28261 gcggcgacca ggccgaagaa accattttgc gagaaaatcc ggatttggtg ttactcgaca
    28321 tcatgctacc aggcaaggac ggcatgacca tttgtcgtga tttacgcgca aagtggtccg
    28381 gaccgattgt tcttctaacc tctctcgata gcgatatgaa ccacatcctg gcactggaaa
    28441 tgggtgcctg cgactatatt ctcaaaacga cgccccctgc tgttttgtta gcgcgtttac
    28501 gtttgcattt gcgtcagaat gagcaagcca cgctgaccaa aggtcttcag gaaacgtcac
    28561 tgactcccta caaagccctg catttcggca cgttgaccat cgatcctatc aaccgcgtag
    28621 tcaccctggc taacactgaa atctcgctct cgacagctga tttcgaatta ttgtgggaat
    28681 tagctaccca tgccgggcaa atcatggacc gcgatgcatt gctgaaaaat ttacgcggcg
    28741 tcagttatga cggcctggat cgtagcgtgg acgtggctat ttcgcggtta agaaaaaaac
    28801 tgctcgataa cgccgctgaa ccttatcgca ttaaaactgt gcgtaacaaa ggctatcttt
    28861 ttgcgcctca tgcatgggaa taagcgatga aaaaactgtt tatccagttt tacctgttat
    28921 tgtttgtctg cttccttgtg atgtctctgc tggttgggtt ggtgtacaaa tttaccgccg
    28981 aacgcgcggg caaacagtcg ctggatgatt tgatgaacag ttcgctgtat ctgatgcgca
    29041 gcgaattgcg tgagatcccc ccacatgact ggggtaagac gctgaaagag atggatttaa
    29101 atctctcttt cgatctgcga gtcgagccac tgagtaaata ccatcttgat gatatttcca
    29161 tgcaccggct gcgtggcggc gaaattgtcg ccctggacga tcagtacact tttttgcagc
    29221 gtatcccgcg cagccactac gtgctggcag ttggtcctgt tccttatctt tattatctcc
    29281 atcagatgcg attgctggat atcgccctga tcgcttttat tgctatttcc ctcgcctttc
    29341 cggtgtttat ctggatgcgt ccgcactggc aggatatgtt aaaactggaa gcagcggcgc
    29401 aacgatttgg cgatgggcat ctcaatgaac gtatccactt tgatgagggt tcgagctttg
    29461 aacgacttgg tatcgcattt aaccagatgg cggacaatat caacgccttg attgccagca
    29521 aaaaacagct tattgacggt atcgctcacg aactgcgaac accgttagtg cgcctgcgtt
    29581 atcgactgga gatgagcgat aacctgagcg ccgccgaatc ccaggcgttg aatcgtgata
    29641 tcagtcaact tgaagcttta attgaagagc tgctgactta tgcccgactc gatcgcccac
    29701 aaaacgagct tcatcttagc gaaccagacc tgccgttgtg gctgtcaacg catctggcag
    29761 atattcaggc agtaacgccc gataaaacgg tacggataaa aacgctcatg caaggccatt
    29821 atgcggcgtt ggatatgcgc ttaatggagc gcgtgctgga taatttgctc aataatgccc
    29881 tgcgctactg ccattcaacg gttgaaacca gcctgctact gtcggggaat agagcgacat
    29941 taattgttga ggatgatggc ccagggattg ccccagaaaa ccgcgaacat atctttgaac
    30001 cttttgttcg cctcgatccc agccgggatc gctcaaccgg cggctgcggg ctggggctgg
    30061 caattgtcca ctctatagca ctggcaatgg gcggtacggt taattgtgac accagcgaac
    30121 tgggtggtgc ccgcttctcg tttagctggc cgttatggca taacatcccg caatttacct
    30181 ctgcttgaca ctacgcgcac ggtggtcaag tcaccaccac tgtgctataa attaaggatg
    30241 ttgtaactaa agtggttaat attatggcgc gttacgatct cgtagaccga ctcaacacta
    30301 cttttcgcca gatggaacaa gagctggctg catttgccgc tcatcttgag caacacaagc
    30361 tattggttgc ccgcgtgttc tctttgccgg aggtaaaaaa agaggatgag cataatccgc
    30421 ttaatcgtat tgaggtgaaa caacatctcg gcaacgacgc gcagtcgcag gcgttgcgtc
    30481 atttccgtca tttatttatt caacaacagt ccgaaaatcg cagcagcaaa gcagctgtcc
    30541 gtctgcctgg cgtgttgtgt taccaggtcg ataacctttc gcaagcagcg ttggtcagtc
    30601 atattcagca catcaataaa ctcaagacca cgttcgagca tatcgtcacg gttgaatcag
    30661 aactccccac cgcggcacgt tttgaatggg tgcatcgtca tttgccgggg ctgatcaccc
    30721 ttaatgctta ccgcacgctc accgttctgc acgatcccgc cactttacgc tttggttggg
    30781 ctaataaaca tatcattaag aatttgcatc gtgatgaagt cctggcacag ctggaaaaaa
    30841 gcctgaaatc accacgcagt gtcgcaccgt ggacgcgcga ggagtggcaa agaaaactgg
    30901 agcgagagta tcaggatatc gctgccctgc cacagaatgc gaagttaaaa atcaaacgtc
    30961 cggtgaaggt gcagccgatt gcccgcgtct ggtacaaagg agatcaaaaa caagtccaac
    31021 acgcctgccc tacaccgctg attgcactga ttaatcggga taatggtgcg ggcgtgccgg
    31081 acgttggtga gttgttaaat tacgatgccg acaatgtgca gcaccgttat aaacctcagg
    31141 cgcagccgct tcgtttgatc attccacggc tgcacctgta tgttgcagat taacgcccgg
    31201 ctttcatact gccgaccatc tgttctggcc gtacccagct gtcaaactcg gcttcgctaa
    31261 gataccccag cgcaagggcc gcagctttta aggtcagccc ttctttatgc gcttttttgg
    31321 cgatctccgc ggctttgtca taaccaatgt gggtgttaag cgcagtcacc agcatcagcg
    31381 attcattgag taattgattg attcgctcac gattcggttc aatacccact gcgcagtgtt
    31441 tgttaaaact ttccatgcca tctgccagca agcgcaccga ttgcaggaaa ttgtggatca
    31501 ccattggacg gaagacgttc agttcaaagt taccggaagc gccccccatg ttgatcgcca
    31561 cgtcgttccc catcacctga cagcagagca tggttaatgc ctcacactgt gttgggttca
    31621 ctttccccgg catgattgag ctgcccggct cattttccgg gattgagatt tcaccaattc
    31681 cgcagcgcgg gccagaggcc agccagcgga catcattggc gattttcatc agtgacgcag
    31741 ccaacccttt caacgcgccg tgcgcctgaa ccagggcatc acaggtcgcc agcgcttcaa
    31801 atttgttcgg cgcggtaaca aacggtgcac aggtaatgac tgccagttca tctgctacgc
    31861 gacgcgcata ctccggatgg gtatttagtc cagtacccac cgctgtaccg ccaagagcca
    31921 gttccgctac gtgaggcagg ctgtattcga tatgtttgag attatgctcg agcatcgcta
    31981 cccagccgga aatctcctgc cccagtgtta gcggggtggc gtcctgcaag tgggttcgac
    32041 cgatttttac gatatcggca aatgcacgcg atttttcact cagcgtctgt gtcagggttt
    32101 taagctgcgg aatgagttgc ttgcgcagcg ccagcagcgc cgcaacgtgc atcgccgtcg
    32161 gaaagacatc gttggaactt tggcttttgt tcacgtcgtc gttagggtga actttacgtt
    32221 ccatcccgcg cacgccgccg agtaattcac tggcccggtt agccagcact tcattcatgt
    32281 tcatgttact ttgcgtgccg gagccggtct gccagatagc cagcgggaat tcgtcgtcat
    32341 gctgtcctgc cagtacttca tccgccgcct gccgaatggc gctcgctttc tcttcagaca
    32401 acaagcctaa atcttcatta acttttgccg ctgcgcgctt ggttagcgcc agcgcatgaa
    32461 tcagtgaggt gggcattttc tccgtcgaaa tgcggaaatg ctccagcgag cgttgagttt
    32521 gtgcgcccca cagcttatct gccgggacat caatcgcccc catcgaatct ttttcgctgc
    32581 gtactgtatt catggcctac tcctcacctg attaattttt tctttctgtt ttgctttcgt
    32641 taagtaactt ttagctcact cattatttac tatttgataa caaatgtttg gtctttcgtg
    32701 ccatgtaaaa aaaccgcccc gaagggcggc tctgtttatt tcacacagcg ggtgcattgt
    32761 gtgagttgta tctgctggaa gaagtcattt cctttatcat ccacaaggat aaacgccggg
    32821 aaatcttcca cttcaatttt ccagatggct tccattccca gttccggata ttcaacacat
    32881 tccaggctct taatacttcc ctgcgccaat acagcggccg gaccaccgat actgccaagg
    32941 tagaagccac cgtgtttttt acaggcatcc gtcacctgct ggctgcggtt gcctttcgcc
    33001 agcatgatca tacttccgcc cagcgcttgc agttgatcga cataagaatc catccgtccg
    33061 gcggtcgttg ggccaagaga accggaggca taaccttccg gcgttttggc cggacccgcg
    33121 tagtaaatcg gatgatcttt gatgtactgc ggcagccctt caccgttatc catccgctct
    33181 ttcagtttgg cgtgagcaat atcacgaccg acgataatcg tgccgttaag cgataagcgt
    33241 gtagaaacgg gatactgcga caactgtgcg aggatctctt tcatcggacg gttaaggtca
    33301 acgcgcaccg cttcgccttc tcccgctttg cgcagctctt ccgggatata tttgcctgga
    33361 ttatgttcca gtttttcgat ccagatcccc tgacggttga tcttcgcttt gatattacgg
    33421 tcagcagaac aggagacgcc cataccgacc gggcaggatg cgccgtgacg tggcaggcga
    33481 atcacgcgga tgtcgtgagc gaagtattta ccgccaaact gcgcacccag accaagattt
    33541 tgcgcttcga tcagcaattc tttttccaat tccacatcgc ggaacgcctg accgtgctca
    33601 ttcccttccg ttggcagttc atcatagtat ttcgcggaag ccagtttcac cgttttaagg
    33661 ttcgtttctg cagaagttcc accaataacg aacgcaatat gatacggagg acaggccgcc
    33721 gtacccagcg tgcgcatctt ctcaaccagg taatttttca gtttccccgg cgtcagtaac
    33781 gctttggttt cctgatagag atacgtcttg tttgccgaac caccaccttt ggcgatacag
    33841 aggaatttgt attcgtcgcc atcaacggca taaagatcga tctgcgctgg cagattggtg
    33901 ccggtattca cttctttata catatccagc ggcgcgtttt gcgagtagcg cagattatct
    33961 tcgatataag tgttatagac accgcgcgcc agcgccgctt catcaccacc accggtccat
    34021 acacgctgcc cttttttacc aacaataatc gcggtgccgg tatcctgaca ggttggcaga
    34081 acgcctttcg ccgcgatgtc ggagttacgc aggaattgca gcgccacata tttatcattt
    34141 tcgctggcct ccgggtcacg cagaatgtcg gccacctgtt gttggtgcgc cggacgcagc
    34201 atgaacgacg catcatgaaa cgcctggcgc gccaacagag ttaacgcttc gggtgcgact
    34261 ttcaaaatct cctgcccttc aaattcagat aagctaacgt gttcgctggt tagcaggtaa
    34321 tactcagtat catctttttt gagtggaaaa ggagcctgat aatgaaaggg tttgtttgac
    34381 attgttttct cacttactgc ctggtttggt tatgctctgg gcgggtgttc cgttgccctg
    34441 ttaaaagcga gtaacaatat cctacgcact tttttaacaa aaactgagac tagtacgact
    34501 ttttgcggct ccaggttact tcccgtagga ttcttgcttt aatagcggga ttaatttcca
    34561 cattaaaaca gggattgatc atgcaaaaac tcattaactc agtgcaaaac tatgcctggg
    34621 gcagcaaaac ggcgttgact gaactttatg gtatggaaaa tccgtccagc cagccgatgg
    34681 ccgagctgtg gatgggcgca catccgaaaa gcagttcacg agtgcagaat gccgccggag
    34741 atatcgtttc actgcgtgat gtgattgaga gtgataaatc gactctgctc ggagaggccg
    34801 ttgccaaacg ctttggcgaa ctgcctttcc tgttcaaagt attatgcgca gcacagccac
    34861 tctccattca ggttcatcca aacaaacaca agtctgaaat cggttttgcc aaagaaaatg
    34921 ccgcaggtat cccgatggat gccgccgagc gtaactataa agatcctaac cacaagccgg
    34981 agctggtttt tgcgctgacg cctttccttg cgatgaacgc gtttcgtgaa ttttccgaga
    35041 ttgtctccct actccagccg gtcgcaggtg cacatccggc gattgctcac tttttacaac
    35101 agcctgatgc cgaacgttta agcgaactgt tcgccagcct gttgaatatg cagggtgaag
    35161 aaaaatcccg cgcgctggcg attttaaaat cggcgctcga cagtcagcag ggtgaaccgt
    35221 ggcaaacgat tcgtttaatt tctgaatttt acccggatga cagcggcctg ttctctccgc
    35281 tgttgctgaa tgtggtgaaa ttaaatcctg gcgaagcgat gttcctgttc gctgaaacac
    35341 cgcacgctta cctgcaaggc gtggcgctgg aagtgatggc aaactccgat aacgtgctgc
    35401 gtgcgggtct gacgcctaaa tacattgata ttccggaact ggttgccaat gtgaagttcg
    35461 aagccaaacc ggctaaccag ttgttgaccc agccggtgaa acaaggtgca gaactggact
    35521 tcccgattcc tgttgatgat tttgccttct cgctgcatga ccttagtgat aaagaaacca
    35581 ccattagcca gcagagtgcc gccattttgt tctgcgtcga aggcgatgca acgttgtgga
    35641 aaggttctca gcagttacag cttaaaccgg gtgaatcagc gtttattgcc gccaacgaat
    35701 caccggtgac tgtcaaaggc cacggccgtt tagcacgggt ttacaacaag ctgtaagagc
    35761 ttactgaaaa aattaacatc tcttgctaag cttattaaag gcttataaca ccttcaggcg
    35821 gccagtccgc ctgatttcat tttatggata atcattatga ataaatcgct ggtagcggta
    35881 ggcgtcattg ttgcgctagg cgtagtctgg acaggcggcg catggtatac aggcaagaag
    35941 attgaaaccc atctcgaaga catggtcgcg caggcgaacg cgcaactcaa actgaccgct
    36001 cctgaatcca acctggaagt gagttatcaa aactatcatc gcggcgtatt cagcagtcag
    36061 ctgcaactgt tggtgaaacc cattgccgga aaagaaaatc cgtggattaa aagcggtcag
    36121 agcgtcatct tcaacgaatc ggttgatcat ggtcccttcc cccttgccca gcttaaaaaa
    36181 ctgaacctga tcccgtcgat ggcatcaatt caaaccacgc tggttaataa cgaagtaagc
    36241 aaaccactgt ttgatatggc aaaaggtgaa acgccttttg agattaactc gcgcattggt
    36301 tacagcggtg attccagttc cgatatttcg ctcaagccac taaattacga gcaaaaggat
    36361 gaaaaagtcg cctttagcgg cggcgagttc cagttaaatg cggacagaga cggcaaagct
    36421 atctcccttt ccggggaggc gcaaagtggt cggatagacg cggttaacga atacaaccag
    36481 aaagtacagt tgacctttaa taatctgaaa accgacggtt ccagcacgct ggcaagtttt
    36541 ggtgagcgcg taggaaacca aaaactgtca ctggaaaaaa tgaccatttc agtggaaggc
    36601 aaagaactgg cactgctgga aggcatggag atcagcggta aatcggatct ggtcaatgac
    36661 ggtaaaacga tcaatagcca actggattac tcgctaaaca gcctgaaggt acagaatcag
    36721 gatctgggca gcggcaagct gactttaaaa gtcggccaaa ttgatggcga agcctggcat
    36781 cagtttagcc agcaatataa cgcgcaaact caggcgctgc tggcacagcc agaaattgcc
    36841 aacaatcccg aactttatca ggagaaagtg agggaagcct tctttagcgc cctgccgctg
    36901 atgttgaaag gcgatccggt gattactatc gcgccgctaa gctggaaaaa cagtcagggt
    36961 gaaagtgcgc tgaatctgtc gctgttcctg aaagatccgg caacgactaa agaagcgccg
    37021 caaacgctgg cgcaggaagt agatcgttcg gttaaatctc tggatgcgaa actgaccatt
    37081 ccggtggata tggcaactga gttgatgact caggtagcga agctggaagg ttatcaggaa
    37141 gatcaagcga aaaaactggc gaaacagcaa gttgaaggtg catcagcaat ggggcagatg
    37201 ttccgtctga ccaccttgca ggacaatacc atcaccacca gcctgcaata tactaacggt
    37261 cagataacgt taaacgggca gaaaatgcca ctggaagatt tcgttggtat gtttgcaatg
    37321 ccggcattaa atgttccggt cgtacccgct attccgcagc agtaattcac agccaaaacg
    37381 ccggagattt ttctctccgg cgttattttt tacttcagca taaagtcata ctgaatactc
    37441 attttcacat cccagtaatt caggcgatcg cccagcgtac tgccatcgcg cttttcccgc
    37501 cctttatcgg cagcaagaaa gaaattcatc ccttgtaacg gcccttcgct gacccgatag
    37561 ttcataccac ctaagatacc cgcgccttca atccgcacat ctcgtttact ggattcatag
    37621 ccgcgagtca caatcggtgc aaatgtcagt gtaaattggg agttaaacga tagttgacgc
    37681 ccaattgcca ggactgcatt ccttcacggt tacgatcaag acttaatgaa aagggatagc
    37741 caatgtcggt atccaccacg gcaccattca ttgcatggcg caaataacca ccactgccaa
    37801 atacgcttcc ccacggcgca ttccaggtca attgtccact aaccaacgcc gtttcattgg
    37861 gctggctggt attacgactt aaaccttcga gttgcgcgta aaagccgagc aactcccctt
    37921 tcaacgtata attcgggtcg agcggtatgc ttttctcgag aatactttgt atctgattgg
    37981 aaacatcttt ctgtaccgat gcccccagag aatagcgact ggtatcatca tgccaactgg
    38041 cggctaaaaa agaacgagcg cgatgtttat cgtattttcc cgtctggctg ttatacaccc
    38101 cttcatcacg aataccggtt gttgaatcag tccataaaaa tactcgtgaa cgccacattc
    38161 cccacagatc aatatttggc gttaaagccc aataacctaa tgctccttca gaaactgtcg
    38221 gtaatgtacg ttgcgaactg ccagccatta acggaatacg ataaggtaag ctaccataac
    38281 tataatccgt cccgaagcga cctaatttaa gcaggctatt ttcactgggt tttattttta
    38341 ctgccccgag ggcaaagcca aaacttttat caccatcaag ataccaccgg gtactcatat
    38401 cagcacgagc acctaattta taaacataat acgctccgcc ttcaacgcca ataatattgg
    38461 aataataacc actattgaaa tcgagcaatc ctccctgtac ccatgcgtaa atatctcttc
    38521 ccgcgccagc actgggctta tcagccctgc gcaattcgtt tttcagtctg atgcgtagtc
    38581 ccgcttcatc gtcagccagt tgcgccgcgt aagcagaggt aaggccagag gcagccgtca
    38641 ggcaaataac ggccatggcc actatttttc tcatagttgc acctttgatg ttctgat
//
LOCUS       DAZFBR010000033        38389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFBR010000033 DAZFBR010000000
VERSION     DAZFBR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.033"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            29..967
                     /gene="ytfI"
                     /locus_tag="ACHJZ2_001938"
     CDS             29..967
                     /gene="ytfI"
                     /locus_tag="ACHJZ2_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFO4568059.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIHHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKSSYQVLSSKNETVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLDDIVSKLKRGKFELQGPGGGIKQCDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     DITKEKICEKYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(1030..1584)
                     /gene="ytfJ"
                     /locus_tag="ACHJZ2_001939"
     CDS             complement(1030..1584)
                     /gene="ytfJ"
                     /locus_tag="ACHJZ2_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO4568060.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1909..2115
                     /gene="ytfK"
                     /locus_tag="ACHJZ2_001940"
     CDS             1909..2115
                     /gene="ytfK"
                     /locus_tag="ACHJZ2_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO4568061.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2211..3554)
                     /gene="paeA"
                     /locus_tag="ACHJZ2_001941"
     CDS             complement(2211..3554)
                     /gene="paeA"
                     /locus_tag="ACHJZ2_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO4568062.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3877..4515)
                     /gene="msrA"
                     /locus_tag="ACHJZ2_001942"
     CDS             complement(3877..4515)
                     /gene="msrA"
                     /locus_tag="ACHJZ2_001942"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO4568063.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4721..6454
                     /gene="tamA"
                     /locus_tag="ACHJZ2_001943"
     CDS             4721..6454
                     /gene="tamA"
                     /locus_tag="ACHJZ2_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO4568064.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6451..10230
                     /gene="tamB"
                     /locus_tag="ACHJZ2_001944"
     CDS             6451..10230
                     /gene="tamB"
                     /locus_tag="ACHJZ2_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO4568065.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGILNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTEEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10233..10574
                     /gene="ytfP"
                     /locus_tag="ACHJZ2_001945"
     CDS             10233..10574
                     /gene="ytfP"
                     /locus_tag="ACHJZ2_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO4568066.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10786..11037
                     /gene="chpS"
                     /locus_tag="ACHJZ2_001946"
     CDS             10786..11037
                     /gene="chpS"
                     /locus_tag="ACHJZ2_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO4568067.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            11031..11381
                     /gene="chpB"
                     /locus_tag="ACHJZ2_001947"
     CDS             11031..11381
                     /gene="chpB"
                     /locus_tag="ACHJZ2_001947"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO4568068.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(11461..11991)
                     /gene="ppa"
                     /locus_tag="ACHJZ2_001948"
     CDS             complement(11461..11991)
                     /gene="ppa"
                     /locus_tag="ACHJZ2_001948"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO4568069.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            12301..13257
                     /gene="ytfQ"
                     /locus_tag="ACHJZ2_001949"
     CDS             12301..13257
                     /gene="ytfQ"
                     /locus_tag="ACHJZ2_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO4568070.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13397..14899
                     /gene="ytfR"
                     /locus_tag="ACHJZ2_001950"
     CDS             13397..14899
                     /gene="ytfR"
                     /locus_tag="ACHJZ2_001950"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO4568071.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14913..15935
                     /gene="ytfT"
                     /locus_tag="ACHJZ2_001951"
     CDS             14913..15935
                     /gene="ytfT"
                     /locus_tag="ACHJZ2_001951"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO4568072.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15922..16917
                     /gene="yjfF"
                     /locus_tag="ACHJZ2_001952"
     CDS             15922..16917
                     /gene="yjfF"
                     /locus_tag="ACHJZ2_001952"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO4568073.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16950..17948)
                     /gene="fbp"
                     /locus_tag="ACHJZ2_001953"
     CDS             complement(16950..17948)
                     /gene="fbp"
                     /locus_tag="ACHJZ2_001953"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO4568074.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            18124..19497
                     /gene="mpl"
                     /locus_tag="ACHJZ2_001954"
     CDS             18124..19497
                     /gene="mpl"
                     /locus_tag="ACHJZ2_001954"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO4568075.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19653..20204)
                     /gene="yjgA"
                     /locus_tag="ACHJZ2_001955"
     CDS             complement(19653..20204)
                     /gene="yjgA"
                     /locus_tag="ACHJZ2_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO4568076.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20298..21650
                     /gene="pmbA"
                     /locus_tag="ACHJZ2_001956"
     CDS             20298..21650
                     /gene="pmbA"
                     /locus_tag="ACHJZ2_001956"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO4568077.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21834..22220
                     /gene="cybC"
                     /locus_tag="ACHJZ2_001957"
     CDS             21834..22220
                     /gene="cybC"
                     /locus_tag="ACHJZ2_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO4568078.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(22265..22729)
                     /gene="nrdG"
                     /locus_tag="ACHJZ2_001958"
     CDS             complement(22265..22729)
                     /gene="nrdG"
                     /locus_tag="ACHJZ2_001958"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO4568079.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22919..25057)
                     /gene="nrdD"
                     /locus_tag="ACHJZ2_001959"
     CDS             complement(22919..25057)
                     /gene="nrdD"
                     /locus_tag="ACHJZ2_001959"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO4568080.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(25451..27106)
                     /gene="treC"
                     /locus_tag="ACHJZ2_001960"
     CDS             complement(25451..27106)
                     /gene="treC"
                     /locus_tag="ACHJZ2_001960"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO4568081.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDERDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(27156..28577)
                     /gene="treB"
                     /locus_tag="ACHJZ2_001961"
     CDS             complement(27156..28577)
                     /gene="treB"
                     /locus_tag="ACHJZ2_001961"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO4568082.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28696..29643)
                     /gene="treR"
                     /locus_tag="ACHJZ2_001962"
     CDS             complement(28696..29643)
                     /gene="treR"
                     /locus_tag="ACHJZ2_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO4568083.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPANLS"
     gene            29828..29881
                     /gene="mgtL"
                     /locus_tag="ACHJZ2_001963"
     CDS             29828..29881
                     /gene="mgtL"
                     /locus_tag="ACHJZ2_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO4568084.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            30022..32718
                     /gene="mgtA"
                     /locus_tag="ACHJZ2_001964"
     CDS             30022..32718
                     /gene="mgtA"
                     /locus_tag="ACHJZ2_001964"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO4568085.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32924..33310)
                     /gene="ridA"
                     /locus_tag="ACHJZ2_001965"
     CDS             complement(32924..33310)
                     /gene="ridA"
                     /locus_tag="ACHJZ2_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO4568086.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(33383..33844)
                     /gene="pyrI"
                     /locus_tag="ACHJZ2_001966"
     CDS             complement(33383..33844)
                     /gene="pyrI"
                     /locus_tag="ACHJZ2_001966"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO4568087.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33857..34792)
                     /gene="pyrB"
                     /locus_tag="ACHJZ2_001967"
     CDS             complement(33857..34792)
                     /gene="pyrB"
                     /locus_tag="ACHJZ2_001967"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO4568088.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34796..34930)
                     /gene="pyrL"
                     /locus_tag="ACHJZ2_001968"
     CDS             complement(34796..34930)
                     /gene="pyrL"
                     /locus_tag="ACHJZ2_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO4568089.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35211..35606)
                     /gene="yjgH"
                     /locus_tag="ACHJZ2_001969"
     CDS             complement(35211..35606)
                     /gene="yjgH"
                     /locus_tag="ACHJZ2_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO4568090.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35737..36450)
                     /gene="bdcA"
                     /locus_tag="ACHJZ2_001970"
     CDS             complement(35737..36450)
                     /gene="bdcA"
                     /locus_tag="ACHJZ2_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4568091.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36521..37114
                     /gene="bdcR"
                     /locus_tag="ACHJZ2_001971"
     CDS             36521..37114
                     /gene="bdcR"
                     /locus_tag="ACHJZ2_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO4568092.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            37259..37711
                     /gene="tabA"
                     /locus_tag="ACHJZ2_001972"
     CDS             37259..37711
                     /gene="tabA"
                     /locus_tag="ACHJZ2_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO4568093.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37834..>38389
                     /gene="yjgL"
                     /locus_tag="ACHJZ2_001973"
     CDS             37834..>38389
                     /gene="yjgL"
                     /locus_tag="ACHJZ2_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO4568094.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAGGQD
                     NTRNILNPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADSPKVIIEISLSTITTMGTRQGHTAIIFPQPDGSTNRYERKSFERKDE
                     SSLHLITNKVLACYQREANKEIARL"
BASE COUNT         9341 a         9468 c        10257 g         9323 t
ORIGIN      
        1 aagtaaccaa ataaaataag gttgtcatat gttacccagg atcagacaca ataattttat
       61 tggtgcggtg gagttatttg taaagtcttc gtatacaaaa acacattcaa acaatttttt
      121 taacaacatc catcatgcat ttaagaaaaa agattggatt tcgaattatg atagcctgtt
      181 aactttgagg gagttctttc gttgcgcaac tcagattgat aaaagtagtt atcaggtgct
      241 ttcatcaaaa aatgaaactg ttaacgctat ggataagttt cttatcagtt tcagccttaa
      301 agataacggt gccgaatata caatgacatt gcgaggcagt ggatttgaat acgaagaaat
      361 ccccataaca ataaatgagt ataactcatt catggatttc aagaaccgtg aatttccgtt
      421 agaacaaaac agacgattgt atgcctggga tattctgcag aaaaaacagt ctgacatacc
      481 gaaaaggatt aaaggttata ttcaccaggc gattggtgac gtgtctttag gttatgcttt
      541 attggatgat atagtaagca aattaaaacg gggtaaattt gaactacaag ggccaggagg
      601 tggaattaaa cagtgcgatg gatggtatat ttacgaaaaa ataattgatg ataattttgc
      661 catagtcata gaatctttgg gctttgcgct taaaatctat ggaggggatg aacgttttcg
      721 taatggttcc tctgttgtct tggaggatga agattactct cttatttata attttctggt
      781 taatgcgggt tgccaacaag tcgaattagc cgaacaagtg gatgcgatag taagtgcgaa
      841 tctggccgca gatagtgata ttaccaaaga aaagatttgc gagaaatata aatcgaccat
      901 tgaggcgttt aaaaaagagc aactagcgtt accagtatta gttcggcgta agaactcaga
      961 gacttaattc ccctatccca tagataacga taggggaaaa ttatttttgc agaatttatg
     1021 ttattgccat tatttgagca acttttgcag caggtccatc acttgctgca cctcttcctg
     1081 agtgagcgcc ccgtctttgg cccattgcac gcgaccgtct ttatccagca ccactacagc
     1141 ggaactttct tcatccagct gccaggcacc gcgcgcgacg ccattgctat cgacaataaa
     1201 ctgcgaccag ggataaagct ttttattact ctccagactg ctgcgcacaa acatgccgga
     1261 acccggaatc gcgtcgtcgg tgttaacaat ggtggtggtc tggtaacgat cgtgcggtaa
     1321 cttcgctgat ttaatcgctt caatcagcgt cgcgtttttc tcttttgcag aggtgcgacc
     1381 agcaatatgt tgcagtactc gcacttttcc cactaactgc gcgctgttcc aggttttgta
     1441 gctaaactga tctttatcaa gcaccaactc gccccgatcg gtaatgccaa tcggcggcac
     1501 tcgctgaccg gtttcgaact gatgtgcgga agccatcatc ggcaacagca ggcaggtgag
     1561 tgccagaatc ttgcgtaggg tcatggtgtt tccttcttat gatatgcagg tgatccgacc
     1621 acttgggtct gagttttaat catatgtgct atttatcgaa tttcccgcaa gtgtgatgcc
     1681 agtttgcggt caagcgcaca aatcatatga aaaatgaatg cttatactga agaccgcgct
     1741 tcggtaaaaa gataattctg aataattgta acctttaggt aaaaaaagtt atacgcggtg
     1801 gaaacattgc ccggatagtc tatagtcact aagcattaaa atttgcgcct cataatagtt
     1861 gggccgattg tggcaccgca caggcgtaat actcagcagg agataacaat gaaaattttc
     1921 caacggtaca acccacttca ggtggcgaag tacgtaaaga tcctgttccg tggacgatta
     1981 tacatcaagg acgttggcgc ttttgaattc gataagggta agattcttat cccaaaagtg
     2041 aaggataagc tgcatttgtc agtgatgtcc gaagttaacc gtcaggttat gcgtctgcaa
     2101 acagagatgg cttaaccaaa gtgctatgca gtaaaaagtg ctatgcagta aaaagtgcta
     2161 tgcagtaata agacggctcc tgattcagga gccgttgatg tttatggggg ttacgcgacg
     2221 ctttcttctt tatctttagc gtcaggcagt tttggcgaaa gggctgtggc cttgctgtcg
     2281 atccgggtca ccagcagctg gtcgatgcgg tagttatcga tatccaccac ttcaaatttg
     2341 tagccggcga atttcaccga atcggtgcgt ttcgggatct tacgcagcat aaacatcata
     2401 aagccgccga tggtttcgta gttgcccgac tgcgggaact cgtcaatatc cagcacgcgc
     2461 atgacgtcgt caatcggagt gccgccgtca atcagccatg agttctcatc acgggcgaca
     2521 atctgctctt ccagtccctg accgaccaga tcgcccatca gcgtggtcat cacgtcgttg
     2581 agggtgatga tccccactac cagcgcgtac tcgttcatga tcaccgcgaa gtcttctcct
     2641 gcggttttaa aactttccaa cgcctctgaa agggttaacg tatccggcac aatcagcgtg
     2701 ttgcgaattt gtacgccgct gttcagtgcc aggctttggt tagccagcac gcggttcagc
     2761 aggtctttag aatcgacata gccgatgatg tggtcaatat cttcattaca gacgaggaac
     2821 ttagagtgcg gatgttccgc caccttattt ttcagacttt gctcatcttc gtggagatca
     2881 aaccaaatca cgttttcacg cggtgtcatt gaagacggaa cggtacggga ttccagctca
     2941 aagacgtttt caatcagctc gtgttcctgt ttacgtaaca cgcccgccag cgcaccggct
     3001 tccactaccg cgtagatgtc atcagaagtg atgtcatctt tacgtaccat tggcagtttg
     3061 aaaatacgga agattatgtt cgccaggccg ttgaagaacc acaccagcgg ggtgcaaacg
     3121 tacaggcaga agcgcatcgg gttgatgata cgcaaagcca cagcttctgg cgcaatcata
     3181 ccgatgcgtt tcggggttaa atccgcaaac aggataaaca tgccagtcac taacgagaaa
     3241 gagagaataa agctcagttg ctcagagagc tctgccgaca tatagcggga gaacaggctg
     3301 tgaaaagctg gagaaaatgc cgcatcaccg acgataccgc cgagaatcgc cactgcgttc
     3361 agaccgattt ggaccacggt aaagaacatg ccgggatttt cctgcatatt cagaacgcgt
     3421 tgggcattta tattgccttc atcagccagc agtttaagtt tgattttgcg tgaggcggca
     3481 agtgagatct cagacatcga gaagaacgca cttacagcga tcaagcagag tatgactaaa
     3541 atactgttta acatatctta tccgaccttt caggccagat cctcggaagg ggaagttgat
     3601 taatttgtgt gaaatacaca ttgaaagccg attcgaagaa agtgaaccgg cattttcagt
     3661 ggctagtata gcgtaaggta ctgtaaagcc gccagagggt taaaattcag acagctgaaa
     3721 aatgcaaaac tgcctgatgc gctatgccga tcaggcctac gcagtccttg caatatattg
     3781 aatttggacg attttgtagg cctggcaagg cgtttacgcc gcatccggca taaacaaaac
     3841 gcactttatc aacaatctac atttgtaccc gtaacgctat gcttccggtg gcagacagac
     3901 gccaattccg ccaattccac agtaaccgta cgggttttta tgcagatatt gctggtggtc
     3961 atcttcggca taataaaacg gtgtggcgtt agcgatttcc gtggtgatgt gacgatcgtc
     4021 atcggcggca agcatcgccg cctgaaaacg ttccagactg gcgcgagctg cggcatcctg
     4081 ttctggggtc agcggataaa tcgctgaacg atactgcgtg ccgtggtcat tgccctgacg
     4141 cattccctgg gcgggatcgt gattctccca aaatacctgt agcaactgct catagctgat
     4201 gacggaagga tcgtaaacaa tgcgtaccgc ttcggcatga ccagtatcac cggagcacac
     4261 ttcccgataa gtcggatttg gcgtatagcc gccggtataa cctgcggcgg tgctgtaaac
     4321 gccgtgtaac tgccagaaca gacgctccac accccagaaa caacccatcg caaaaatggc
     4381 aatctccatt ccgtcaggta cattggtcat tgagtgaccg ttaaccgcat gcagcgtggc
     4441 tacgggcatc ggggtgttac gtccaggcag ggcatcggcg ggggaaacca gatgcttttt
     4501 atcaaataaa ctcatggtgt cgctctcccg aaaatcggtc attggggtta aggttgtaac
     4561 aagaggcgta tttgcacaca ataaccaccg tgaataggtc taaagtaaaa cataagaaat
     4621 atttggggtt tagtctgctt tttaatccat attactggat ttttgttaag ccgtttaacg
     4681 gcgttccagg ggcaggaaaa aaggatattc aggagaaaat gtgcgctata tccgacagtt
     4741 atgctgtgta agcttactct gcttaagcgg atctgccgtc gccgcgaacg tccgtctaca
     4801 ggtcgagggg ttatcgggac agctggaaaa gaacgttcgt gcgcagcttt ctacgattga
     4861 aagtgatgaa gtgacgccag accgtcgctt tcgcgcacgc gtcgatgatg ctatccgcga
     4921 aggtctgaaa gcgctgggtt attaccagcc gaccattgaa tttgatctcc gtccaccgcc
     4981 aaagaaaggg cggcaggtat tgatcgccaa agtcacgcca ggcgtgccgg tgttaattgg
     5041 cggcaccgat gtggtattgc gcgggggcgc gcggaccgat aaagactatt tgaaattgct
     5101 cgatactcgc ccggctattg gcacggtact gaaccagggc gattatgaaa atttcaaaaa
     5161 gtccttaacc agcattgcgt tgcgtaaagg ttatttcgat agcgaattta ccaaagcgca
     5221 gctgggcatt gcgctcggcc tgcataaagc cttctgggat attgattata acagtggcga
     5281 acgttaccgc tttgggcatg tgacctttga aggatcacaa atccgcgatg aatacctgca
     5341 aaatctggtg ccgtttaaag agggcgatga gtacgaatcg aaagatctgg cagaactgaa
     5401 ccgccgactt tctgctaccg gctggtttaa ctcggtggtg gtggctccac aatttgataa
     5461 agcgcgcgaa acgaaagtat taccattgac gggcgtggtt tcgccgcgaa cagaaaacac
     5521 tatcgaaacc gggggcggtt actctacgga cgtgggaccg cgcgtgaaag cgacgtggaa
     5581 aaaaccgtgg atgaactcat acggtcacag tctgaccacc agtaccagta tttccgcgcc
     5641 ggaacagacc ctcgacttca gctataaaat gccgctgctg aagaatccac tggaacaata
     5701 ttatttggtg cagggcggtt ttaagcgcac tgacctgaac gataccgagt ctgactccac
     5761 tacgctggtg gcttctcgct actgggatct ctccagcggc tggcagcgtg ccattaacct
     5821 gcgctggagt ctcgaccact ttactcaggg tgaaattacc aacaccacga tgctgtttta
     5881 tcctggggtg atgattagcc gcacgcgttc tcgtggtggc ctgatgccaa cctggggcga
     5941 ctcgcaacgc tactctatcg actactccaa cacggcctgg ggttcagatg tcgatttctc
     6001 cgttttccag gcgcagaacg tctggatccg cacactgtac gatcgccatc gttttgtgac
     6061 acgcggcacg ctgggctgga ttgaaaccgg tgatttcgac aaagtaccgc cggatctgcg
     6121 tttcttcgcc gggggcgatc gcagtattcg cggctacaaa tacaaatcta tcgccccgaa
     6181 atacgctaac ggtgacctga aaggggcctc gaagttgata accggatcgc tggaatacca
     6241 gtacaacgtg accggaaaat ggtggggcgc ggtgtttgtc gatagtggcg aagcggtaag
     6301 cgatattcgc cgcagcgact ttaaaaccgg taccggggtc ggcgtacgct gggaatcgcc
     6361 ggtcgggcca atcaaactcg attttgccgt accggtcgcg gataaagacg aacacgggtt
     6421 acagttttac atcggtctgg ggccagaatt atgagtttat ggaaaaaaat cagcctcggc
     6481 gtggttatcg ttatcttact gttgctggga tcggtggcgt ttctggtggg caccaccagc
     6541 ggcctgcatc tggtatttaa agcggcggat cgctgggtgc caggactgga tattggcaag
     6601 gtcaccggcg gctggcgcga tctcaccttg tctgacgttc gttatgagca gccaggcgtg
     6661 gcggtaaaag cgggtaatct gcatctggcg gtcggtcttg agtgcctgtg gaacagcagt
     6721 gtttgtatta atgacttggc gctgaaagac attcaggtca acatcgacag taaaaaaatg
     6781 cctccttctg aacaggttga agaagaggaa gatagcggtc cgctggatct ctccacgccg
     6841 tatcccatca ccctgacacg ggtggcactg gacaacgtca acatcaagat tgatgacacc
     6901 acggtatcgg tgatggactt cacctccggc ctgaactggc aggagaaaac cctgaccctg
     6961 aaaccgacgt cgctgaaagg cctgctgatt gctctgccga aagtggcgga agtggcgcag
     7021 gaagaagtgg tcgaaccgaa aattgaaaat ccgcagccgg aggaaaagcc tctcggcgaa
     7081 acactcaaag atctcttttc tcgcccggta ttgccggaaa tgaccgacgt gcatctgcca
     7141 ctcaacctga acattgaaga gtttaaggga gagcaactgc gcgtgacggg cgatacggac
     7201 attaccgtgc gcaccatgct gctgaaagtg agcagcattg acggcaatac taaactggac
     7261 gccctggata tcgattccaa tcaagggatc ctcaacgcca gcggcacggc gcagctgtca
     7321 gacaactggc cggtggatat caccctcaac agcacactga acgtggagcc gttgaaaggt
     7381 gaaaaggtga agctgaaagt gggcggcgcg ctgcgcgaac agctggagat tggcgttaat
     7441 ctttccggtc cggtggatat ggatttacgc gcccagacgc gactggcgga agccggattg
     7501 ccgctcaacg tggaagtgaa cagcaaacag atttactggc cgttcactgg tgagaagcag
     7561 tatcaggcgg atgatctgaa actgaaactt accggcaaaa tgaccgatta cacgctctct
     7621 atgcgtacgg cagtgaaggg actggagatc ccgccagcca ccattaccct tgatgccaaa
     7681 ggtaatgaac agcaggtcaa tctcgacaaa ctcaccgtcg cggcgctgga agggaaaact
     7741 gaactcaagg cgttgcttga ctggcagcag gccattagtt ggcgcggtga gctaacgctt
     7801 aacggcatta acaccgccaa agagatccca gagtggccgt cgaaactcaa tggcttgatt
     7861 aaaacccgcg gtagcctgta cggcggcacc tggcagatgg aggtgccaga actgaagctt
     7921 accggtaacg tcaaacagaa caaagtgaac gttgacggca cgctgaaagg caacagttat
     7981 atgcagtgga tgatcccagg gcttcatctg gaactggggc caaacagtgc cgaagtgaaa
     8041 ggcgagctgg gggtaaaaga tctcaatctt gatgccacca tcaacgcgcc ggggctggat
     8101 aacgcgctgc cggggcttgg cggtacagcg aaagggctgg tgaaagttcg cggcacggtg
     8161 gaagcgccac aactactggc ggacatcacc gcgcgcggcc tgcgctggca ggagctaacc
     8221 gtggcgcagg ttcgcgtgga aggcgatatc aaatccaccg atcagattgc gggtaaactc
     8281 gacgtgcgcg ttgagcaaat ttcacagccg gatgtaaata tcaacctcgt caccctgaat
     8341 gccaaaggca gcgaaaaaca gcacgagtta cagttgcgga ttcagggcga gccggtttcc
     8401 gggcagctta atctggcagg aagttttgat cgcaaagaag aacgctggaa gggaactctt
     8461 agcaataccc gcttccagac gctggtcggc ccgtggtcgc tgacccgcga tattgcgctg
     8521 gattaccgca ataaggagca aaaaatcagc atcgggccac actgctggct taacccgaat
     8581 gcggaactgt gcgtgccgca aactatcgat gcgggggccg aagggcgtgc ggtggtgaat
     8641 ctcaaccgct tcgaccttgc catgctgaaa ccgtttatgc cagaaaccac tcaggccagc
     8701 ggtatcttca cgggtaaagc ggacgttgcc tgggacacca ccgaagaggg gctgccgcag
     8761 ggcagtatca ccctttcggg gcgtaacgtg caggtaacgc aaaccgtcaa cgatgcggcg
     8821 ctgcccgtgg cgtttcagac actgaatctg acggcggaat tgcgtaacaa ccgtgccgaa
     8881 ttgggctgga ccatccgcct gaccaataac ggccagtttg atggacaggt gcaggtgacc
     8941 gatccgcaag gccgccgtaa tcttggtggc aacgtcaata tccgtaactt caaccttgcg
     9001 atgataaacc ccatctttac ccgtggggaa aaagcagcgg ggatggtgag tgccaacttg
     9061 cgtctgggtg gtgatgtgca aagcccgcag ttgtttggtc agcttcaggt tacgggtgtg
     9121 gatatcgatg gcaactttat gccgtttgat atgcaaccga gccagcttgc ggtcaacttt
     9181 aacggtatgc gctcgacgct tgccggtaca gtacgaaccc agcagggtga aatctacctg
     9241 aacggtgatg ccgactggag ccaaattgaa aactggcggg cgcgggtaac ggcgaaaggc
     9301 agtaaagtgc ggatcaccgt gccgccgatg gtacgaatgg atgtatcgcc agatgttgta
     9361 ttcgaggcta caccaaacct gtttaccctc gatggtcgcg tggatgtccc ttgggcgcgc
     9421 atcgtggtgc acgatctgcc ggaaagcgca gtaggcgtct ccagcgatgt ggtgatgctt
     9481 aacgataacc tgcaaccgga agagccgaaa acggcgtcga ttccgattaa cagtaacctg
     9541 attgtccacg ttggcaacaa tgtgcgcatt gacgcctttg gcctgaaagc gcggctgacg
     9601 ggcgatctta acgtcgttca ggacaaacaa gggctgggcc tgaacgggca gatcaacatc
     9661 cctgaagggc gcttccatgc ctatggtcag gatctgattg tgcgtaaagg tgagttactg
     9721 ttctctggcc cgccggatca accgtatctt aatattgaag ctattcgtaa cccggatgct
     9781 acagaagacg acgtaatcgc cggagttcgc gtcactggtc tggcggacga accgaaagcg
     9841 gagatcttct ctgacccggc gatgtcgcaa caagctgcct tgtcttattt gctacgtgga
     9901 caagggctgg agagcgatca gagcgacagt gcggcaatga cctcgatgct gattggtctg
     9961 ggggttgcgc aaagtggcca gattgtaggt aaaatcggcg agacgtttgg cgtaagcaat
    10021 ttagcgctcg acacccaggg agtaggcgac tcctcccagg tagtggtcag cggctatgta
    10081 ttgccaggtc tgcaagtgaa atacggcgtg ggtatatttg actctatagc aacactcacg
    10141 ttacgttatc gcctgatgcc taagctatat ctggaagccg tgtctggtgt agaccaggca
    10201 ctggatttgc tctatcagtt cgagttttag caatgcgaat atttgtctac ggcagtttac
    10261 gccacaaaca aggcaacagt cactggatga ccaatgccca gttactgggc gatttcagta
    10321 tcgataacta ccagttgtat agcctgggcc actatccagg cgcagttccg gggaacggaa
    10381 cggtacacgg tgaagtttat cgtattgaca acgccacgct ggccgaactt gatgccttgc
    10441 gcaccagggg cggtgaatac gcgcgccagt tgattcagac gccgtacggg agtgcatgga
    10501 tgtacgttta tcaacgaccc gtcgatggat taaagctaat tgaaagcggc gactggttag
    10561 acagggataa gtaaccctat gcatacgcca ccttcgggtg gcgttgtttt ttgcgagacg
    10621 actcgcattc tgttttgtaa ttccctcacc ttttgctttt ctctccgagc cgctttccat
    10681 atctattaac gcataaaaaa ctctgctggc attcacaaat gcgcaggggt aaaacgtttc
    10741 ctgtagcacc gtgagttata ctttgtataa cttaaggagg tgcagatgcg tattaccata
    10801 aaaagatggg ggaacagtgc aggtatggtc attcccaata tcgtaatgaa agaacttaac
    10861 ttacagccgg ggcagagcgt ggaagtgcag gtgagcaaca accaactgat tctgacaccc
    10921 atctccaggc gctactcgct tgatgaactg ctggcacagt gtgacatgaa cgccgcggaa
    10981 cttagcgagc aggatgtctg gggtaaatcc acccctgcgg gtgacgaaat atggtaaaga
    11041 aaagtgaatt tgaacgggga gacattgtgc tggttggctt tgatccagca agcggccatg
    11101 aacagcaagg tgctggtcga cctgcgcttg tgctctccgt tcaagccttt aatcaactgg
    11161 gaatgacgct ggtggccccc attacgcagg gtggaaattt tgcccgttat gccggattta
    11221 gcgttccttt acattgcgaa gaaggcgatg tgcacggcgt ggtgctggtt aatcaggtgc
    11281 ggatgatgga tctacgcgcc cggctggcaa agcgtattgg tctggctgcg gatgaggtgg
    11341 tggaagaggc gttattacgc ttgcaggcgg tggtggaata aggtgtgttt atttatcgcg
    11401 ggcataaaaa aacccttact aaccgaagcc cggcgttcag ggttattacg ccagaagaac
    11461 ttatttattc tttgcgcgct cgaaggaggc aacgatttca gctttagcgg cttctgcgtt
    11521 ttcccaacct tcaactttca cccacttgcc tttttcgagg tctttgtagt gctcgaagaa
    11581 gtgagcgatt tgcgctttca gcagttcagg cagatcgtta acgtctttaa tgtgatcgta
    11641 ttctttgctc agcttgctgt gcggaaccgc aaccagtttc gcatcttcac cggcttcgtc
    11701 ggtcattttc agaacgccaa ccggacggca acggatcaca gaacccggct gcagcgggta
    11761 cggagttggg accagtacgt caactgggtc accgtccaga gacagggtgt ggttgatgta
    11821 accgtagttg cacggataga acatcgcggt ggacatgaag cggtcaacga acagtgcgcc
    11881 gctctctttg tcgatttcgt atttgatcgg atctgcgtta gccgggatct caataacaac
    11941 gtagatgtct tccggcagat ctttacccgc agggacgttg agtaagctca tgtctgtttc
    12001 ctttaaaaat atgtggcaaa caagtgccga gtattatagc caactcgcgc cgaatgtctt
    12061 cgcttgtttt cgtcttcatt ttcctttttc gccagtttta agacggtatg cagagcagga
    12121 aaatccataa ccgtagccgc atttttcata gtgagatgaa agcgattaca aacttgtgat
    12181 taacgtttta tttacttttt tgaagtgtga tgtaacgcaa tccgttacat aacgaattgt
    12241 ctatagtttt ttcgcgaaca tcttttcacc aataataatt actccgacga ggacaaccct
    12301 atgtggaaac gcttacttgt agtctctgca gtctcggcag ccatgtcgtc tatggcgttg
    12361 gccgctccat taaccgtagg attttcgcag gtcggatcgg aatccggctg gcgcgccgca
    12421 gaaaccaatg tggcgaaaag tgaggccgaa aaacgcggaa ttacgctgaa aattgccgat
    12481 ggtcagcaaa agcaggaaaa ccagattaaa gcggtacgtt ccttcgttgc acaaggggtg
    12541 gatgcgatct ttatcgctcc ggtggtcgcg acaggttggg aaccggtatt aaaagaggcg
    12601 aaagatgccg aaatcccggt cttcttgctc gaccgttcca tcgatgtgaa agacaaatct
    12661 ctctatatga ccaccgttac tgccgacaac atcctcgaag gtaagttgat cggtgactgg
    12721 ctggtaaaag aagtgaatgg caaaccatgc aacgtggtgg agctgcaggg caccgttggg
    12781 gccagcgtcg ccattgaccg taagaaaggc tttgccgaag ccattaagaa tgcgccaaat
    12841 atcaaaatca tccgctcgca gtcaggtgac ttcacccgca gtaaaggcaa agaagtcatg
    12901 gagagcttta tcaaagcgga aaacaacggc aaaaacatct gcatggttta cgcccataac
    12961 gacgacatgg tgattggtgc aattcaggca attaaagaag cgggcctgaa accgggcaaa
    13021 gatatcctca cgggttccat tgacggtgta ccggacatct acaaagcgat gatggatggc
    13081 gaagcgaacg ccagtgttga actgacgccg aacatggcgg gacccgcttt cgatgcgctg
    13141 gagaaataca aaaaagacgg caccatgcct gaaaagctga cgttaaccaa atccaccctt
    13201 tacctgcctg ataccgcaaa agaagagtta gagaagaaga aaaatatggg gtattgaggg
    13261 ttgctatgcc tgatgccgat tcgtaggccg gataaggcgc tcgcgccgca tccggcgatg
    13321 gtgcactgaa gcctgatgcg acgcttaccg cgtcttatca tgcctactgg gagcacgctt
    13381 tacaccgggg gaaaccatga cgaccgacca acaccaggag atcctccgca ccgaaggatt
    13441 aagtaaattt ttccccggcg tcaaagcgtt agacaacgtt gatttcagcc tgcgccgtgg
    13501 cgaaatcatg gcgctgctcg gtgaaaacgg ggcgggaaaa tcaacgctaa tcaaagcatt
    13561 aactggtgta taccacgctg atcgcggcac catctggctg gaaggccagg ctatctcacc
    13621 gaaaaatacc gcccacgcgc aacaactcgg cattggcacc gtctatcagg aagtcaacct
    13681 gctacccaat atgtcggtcg ctgataatct atttataggc cgcgaaccca aacgcttcgg
    13741 ccttctacgc cgcaaagaga tggaaaagcg cgccaccgaa ctgatggcat cttacggttt
    13801 ctccctcgac gtgcgcgaac cgctcaaccg cttttcagtc gcgatgcagc aaatcgtcgc
    13861 tatttgccgg gctatcgatc tctctgccaa agtgctgatc ctcgatgaac ccaccgccag
    13921 tctcgacacc caggaagtgg agttactgtt tgacctgatg cgtcagttgc gcgatcgcgg
    13981 cgtcagcctg atctttgtca ctcactttct cgatcaggtc tatcaggtca gcgatcggat
    14041 caccgtctta cgcaacggca gtttcgtagg ctgtcgggaa acgtgcgagc taccgcagat
    14101 cgaactggta aaaatgatgc tggggcgcga gctggatacc cacgcgctac agcgtgccgg
    14161 gcgaacattg ttgagcgaca aacccgttgc cgcgttcaaa aattacggca aaaaaggaac
    14221 gatcgcaccg tttgatctcg aagtacgccc cggcgagatc gtcggtctgg ctggattgct
    14281 gggatcagga cgtaccgaaa ccgccgaagt gatcttcggt atcaaacctg ctgacagcgg
    14341 cacggcgttg atcaaaggca aaccgcaaaa cctgcgatcg ccacatcagg cttcggtact
    14401 tggcattggc ttctgcccgg aagacaggaa aaccgatggc atcatcgctg ccgcctcggt
    14461 gcgggaaaat atcatcctcg ctctccaggc ccagcgcggc tggctacgtc ccatttcccg
    14521 caaagaacag caagagattg ccgaacgctt tatccgccag cttggcattc gcacaccttc
    14581 aactgaacaa ccgattgaat ttctctccgg cggcaatcag caaaaagtgt tgctttcacg
    14641 ttggctactg acccgaccgc aatttctgat cctcgatgag ccaacccgcg gcattgatgt
    14701 tggtgcccac gccgagatca tccgcctgat tgaaacgcta tgcgccgatg gtctggcgct
    14761 gctggtgatc tcctccgaac tggaagaact ggtgggctat gccgaccggg tgatcatcat
    14821 gcgcgatcgc aaacaggtgg cggagatccc gctggcagag ctttccgttc cggcgatcat
    14881 gaatgccatt gcggcgtaag gagaacagtg tgatgcctca atctctcccg gacactacgc
    14941 cgccaaaaag gcgctttcgc tggccaacgg gaatgccgca gctggcggca ctgttgctgg
    15001 tgctgctggt cgatagcctg gtggccccgc atttctggca ggtggtgctt caggatgggc
    15061 gtttgttcgg tagccctatc gacattctta atcgcgccgc ccccgttgcg ctactggcga
    15121 ttggtatgac gctggtgatc gcaacaggtg ggattgatct ctccgtaggg gcggtgatgg
    15181 ctatcgccgg agccacaacg gctgcgatga cggtcgcggg attcagcctg ccgattgttt
    15241 tgttaagcgc cctgggcacc ggcatcctgg cgggattgtg gaacggcata ctggtagcga
    15301 tcctcaaaat tcagccgttt gtcgccactc tgatcctgat ggtcgccggg cgcggcgtgg
    15361 cgcaactgat cacctccgga cagatcatca cgtttaactc gccggatctc tcatggttcg
    15421 gcagtggatc gctgttgttc ctgccaacgc cggtcattat cgcggtgctg acgcttatcc
    15481 tgttctggct gttgacccgc aaaacggcgc tggggatgtt tatcgaagcc gttggtatca
    15541 acattcgggc ggcaaaaaat gccggggtaa acacgcggat catcgtcatg cttacctacg
    15601 tgttgagcgg gctgtgtgcg gcgattgcgg gcattatcgt ggcggcggat attcgcggtg
    15661 ccgatgccaa caacgccgga ttatggctgg agctggacgc cattctcgcg gtggtgattg
    15721 gcggtggttc gctgatgggc ggacgtttta acctactgct ttcggtggtg ggggcgctga
    15781 ttattcaggg gatgaacacc ggaattttgc tttcgggctt tccgccagag atgaaccagg
    15841 tggtgaaagc ggtggtggtg ctttgcgtgc tgattgtcca gtcgcaacgc tttatcagtc
    15901 tgattaaagg agtgcgtagc cgtgataaaa cgtaatttgc cactgatgat caccatcggc
    15961 gtctttgtgc tgggttatct ctactgcctg acccagtttc ccggttttgc ttccacaaga
    16021 gtgatctgca atatcctgac cgataatgcc tttcttggga taatcgccgt tggcatgacc
    16081 tttgtgatcc tctccggtgg gatcgatctc tccgttggtt cggtgatcgc ctttaccggc
    16141 gtgtttctgg caaaagtgat tggcgatttc ggcctctcgc cgttgctggc atttccgctg
    16201 gtgctggtaa tgggctgtgc ctttggcgca tttatggggc ttttgatcga cgccctgaag
    16261 atcccggcat ttatcattac tttggcagga atgttctttt tgcgcggcgt cagctacctc
    16321 gtttcggaag agtcgatccc gataaaccat cctgtttatg acacgctctc aagccttgca
    16381 tggaaaattc ctggcggcgg tcgcttaagt gcgatggggc tgctgatgct ggcggtggtg
    16441 gttatcggca tattcctcgc gcatcgtacc cgctttggta atcaggtgta cgccattggc
    16501 ggcaatgcga cgtcggcaaa cctgatgggg atttccactc gcagcacaac tattcgcatt
    16561 tatatgctct ctaccggact ggcgacactg gcggggattg tcttctcgat ttatacccag
    16621 gccggatacg cgctggcggg cgtaggtgtg gaactggacg ctatcgcctc agtggtgatt
    16681 ggcggtacgc ttttgagcgg tggcgttgga acggtattag ggacgctttt tggcgtggcg
    16741 attcagggac tgattcagac ttacataaac tttgatggta cgctgagctc atggtggacg
    16801 aaaatcgcca tcggtatttt gttgtttatt tttatagcat tacagcgtgg attaacggtg
    16861 ctgtgggaga atcgtcagag ttcgcctgtg acaagagtca acattgcgca gcaataaaat
    16921 cgcctctccg tgtggagagg cgcaggagat tacgcgtccg ggaactcaca gataaagcgt
    16981 tcgacatctt caaccatatg gtcgttgccg acaaagaatg aacggcgctg gtgcagggtt
    17041 tccgggataa tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc
    17101 gccaggaatg ccatcgggtt gcactcatac agcaaacgca gtttgccgtc cgggtggctg
    17161 gcggtgcttg ggtagagata aataccgcct ttcagtaggt tacggtggaa atccgcaacc
    17221 agtgagccga tataacgtga ggtatacggg cggttggtgg atttatcttc ttcctggcag
    17281 aatttaatgt acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag
    17341 taggttttgc ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgccgagc
    17401 gaaggatcgt aagtaaaagc gtgaacaccg catccggtac tgtaaaccag catggtagag
    17461 gagccgtata ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct
    17521 tccgttaccg gcgtgccgac gggcgtaact cggcggtaga tggagaaaat ggtaccgaca
    17581 gagacgttaa catcgatgtt ggacgagcca tccagtgggt ccatcagcac cacgtatttt
    17641 gcatgttcac agccttcaaa gacaacaatc tcatcttctt cttcagaggc aatgcctgca
    17701 acgatatcgc gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt
    17761 tgctgaacct cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct
    17821 gctttgttga tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca
    17881 gtgagctcac cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac
    17941 gttttcataa aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca
    18001 aataatctca atgttgcgct caggtgaatc gcgccagcaa attacggatt atcctgaaat
    18061 gcgtttctca cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga
    18121 cgcatgcgca ttcatatttt aggaatttgt ggcacgttta tgggcggtct ggcgatgctg
    18181 gcgcgccagt taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc
    18241 accttacttg agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgac
    18301 ccgcagccgg atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa
    18361 gcggtactgg aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg
    18421 ctgcgcgacc gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga
    18481 atggcgactt ggattctgga acagtgtggt tacaaaccgg gctttgtaat cggcggtgtg
    18541 ccggggaact ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg
    18601 gatgagtacg actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt
    18661 acgctgatcc tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg
    18721 atccagaaac agttccacca tctggtgcgt atcgttccgg ggcagggtcg tattatctgg
    18781 ccggaaaatg acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag
    18841 ctggtgggtg agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg
    18901 gaagttttgc tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat
    18961 aatatgcaca atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca
    19021 gatgccgcta acgcgctggg ttcgtttatt aacgctcgcc gccgtctgga gttgcgtggt
    19081 gaagcgaatg gcgtcacggt atatgacgat tttgcccatc atccgacggc gattctggca
    19141 acgctggcgg cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggag
    19201 ccgcgctcga ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt
    19261 cgtgccgatg aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg
    19321 gcagaagcct gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg
    19381 gtggtgaaaa ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt
    19441 gggatccatc agaaactact ggatggtctg gcgaagaagg cggaagctgc gcagtaattc
    19501 ggcctcagcc tgagatagtg tgcgtaggcc ggataaggcg ttcacgccgc atccggcatt
    19561 tgagcatagt gcctgatgcg acgcttgatg cgtcttatca ggcctacagg tgcaccgcat
    19621 ccggcaaacc atactcacat caacaacgaa aattaccctt cattctccgc cagttcgcgc
    19681 agatactgga aaatctggcg tgcggatttc ggcggcttat tcccttcttt ctctttcttc
    19741 gcgttgcgga tcagagtacg cagttgctga cgatcggcat cgggccacag atttaatacc
    19801 tcagcgatgg cgtcatcacc ttgatcgatc agacgatcgc gcaggttttc cagtttatgg
    19861 aacagcacca cctgctggtt gtgacggttt ttcagcttat ccagcgcctg acggatcggt
    19921 tctacatcgc gctggcgcag catcttaccg atgagctgca actggcggcg gcgaccttcc
    19981 atcttaatac gctgggcgag ctcaatagcc gcgcgcagat ccgcatctaa cgggatctta
    20041 tccagcgcgt ttttccccag atcaacaatt tccgcgccaa ggcgttttag ctcctcggca
    20101 tcacgtttaa tttcactttt actgacccag ataatttcat cgtcttcgtc ttcgatgtca
    20161 tcaccgggaa cgtcgtcgag ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa
    20221 aaagaggcta atgttaccag ttaagatgcg cactgaaaaa cggttctctg ttagacttca
    20281 gagaaactct ctacattatg gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc
    20341 gcaagattct ggaagaagca gtttccactg cgctggagtt ggcctcaggc aaatcggacg
    20401 gtgcggaagt tgccgtcagc aagaccaccg gcattagcgt aagcacgcgt tatggtgaag
    20461 tggagaatgt tgaattcaat agcgatggcg cgctggggat cactgtttat caccagaacc
    20521 gcaaaggtag cgcatcatcc accgatttaa gcccgcaggc cattgcccgt actgtacagg
    20581 cggcgctgga tattgcccgt tatacctcgc cagatccctg tgccggcgtg gcagacaaag
    20641 agctgctggc ctttgacgca ccggatctcg acttgttcca ccctgcggaa gtttccccgg
    20701 atgaagccat tgaactggcg gcccgcgcag aacaggcggc attgcaggcg gacaaacgca
    20761 tcaccaatac cgaaggtggc agctttaaca gccactacgg tgtcaaagtt tttggcaaca
    20821 gccacggcat gttgcagggt tactgctcaa cgcgtcattc gctctccagc tgtgtaattg
    20881 ccgaagaaaa tggcgatatg gagcgtgatt acgcctacac cattggccgc gcgatgagcg
    20941 atttgcagac gccagagtgg gtcggggccg attgcgctcg ccgtacttta tcgcgtctgt
    21001 caccgcgtaa actctccacc atgaaagcgc cggtcatttt tgccaatgaa gtggcaaccg
    21061 ggctttttgg gcatctggtg ggggcgatag cgggtggatc ggtttaccgt aaatctacct
    21121 tcctgctgga ttcgctgggt aaacaaattc tgccggactg gctgaccatt gaagagcatc
    21181 cgcatctgct gaaagggctg gcatcgacgc catttgacag cgaaggtgtg cgcaccgagc
    21241 gtcgcgatat tattaaagat ggcatcctga ctcagtggct gctgaccagc tactcggcgc
    21301 ggaaactggg gctgaaaagc accggacatg cgggcggtat tcacaactgg cggattgccg
    21361 gacaaggtct aagcttcgag cagatgctca aagagatggg caccgggctg gtggtgacgg
    21421 aattgatggg ccagggcgtg agtgcaatta ccggtgatta ttcccgtggt gcggcgggct
    21481 tctgggtaga aaacggcgaa attcagtatc cggttagcga aatcaccatc gcaggtaatt
    21541 taaaagatat gtggcgcaat attgtcaccg tcggtaacga tattgaaaca cgcagtaata
    21601 tacagtgtgg ttctgtgctg ttgccggaga tgaaaatcgc cggacagtaa aaaaactggc
    21661 gcgacctgcc gcgctataaa ctaaaaaatt acacaaaatc attcgcactg catcgagacg
    21721 gaaagtgaat gagggccggg gagcgtactc gcagtacgtg acccggtcga atgagcgtag
    21781 tcgtcgatga ggcagtgtga aggatgaagt gtaaataaaa aaggaagtga gcaatgcgaa
    21841 aaagcctgtt agctattctt gcagtctcct cgttggtatt cagttcggcg tcgtttgctg
    21901 ctgatcttga agacaatatg gaaaccctca acgacaattt aaaagtggtc gaaaaagccg
    21961 ataacgcggc gcaagtcaaa gacgcgttaa cgaagatgcg cgccgcagcg ctggatgcgc
    22021 aaaaagcaac gccgccgaag ctcgaagata aatcaccgga cagcccggaa atgaaagatt
    22081 tccgccacgg tttcgacatt ctggtcggtc agattgacga cgcgctgaag ctggcaaatg
    22141 aaggtaaagt aaaagaagcg caggctgctg cagagcaact gaaaacgacc cgcaattcat
    22201 atcacaagaa gtatcgttaa ttcctcattt ccctgttgcc tgcactcagg taacagggaa
    22261 cttatcatcg caaatgatgc accacctgat tgctgctgcc gcgccagatc agggaagggt
    22321 cttttaaatc ctgcacaaat ttgccgtcga ccagcacgtt aatcaaatca acaacctgca
    22381 tttgcgcagc gttgagttcg tcgagtttat agcctgtcca cacccagatg tctttacccg
    22441 gacactcggc gcggatgcgt tgtaccagtt tcagaatatc cggcacgttt tgcggatgca
    22501 gcggatcgcc gccggagagg gaaataccct ggcgtttgat acgagtgtca ttcagatcgt
    22561 taatgatctg gtcttccatt gctttagtaa atggctgacc ggaatttacc cgccatgtgc
    22621 ttttgttata gcaaccgggg cattcatgaa cacacccgga gacaaacagg gtgcagcgag
    22681 tgccgggacc gttgacgatg tcgacaggat agtactgatg ataattcatt atttcgcctg
    22741 gcttatcagt ccgcgtgtag gctgtatcag gcaatcaacg cctgatgcga cgctgtcgcg
    22801 tcttatcagg cctacagccg ttgccaaatg taggccggat aaggcgttta cgccgcatcc
    22861 ggcaatcaac gcgggatgcg acgctttcac atcacatccg gcatttgctt agtgcagatc
    22921 aacctatctg cccatttccc aaatgtttaa cgcggcgctt aacttcttcc tgcttgccgg
    22981 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gttaccgaca
    23041 cacgggaggc gtcatggtta ccacatttcg ggcaggtgaa gcctttgctg gtgcactcga
    23101 actcaccggt aaagccacac tcgtagcact catcaatcgg tgtattggtg ccgtaatacg
    23161 gtacatgctg atagctgtaa tcccagacat cttccagcgc cttcaggttg tgctgaatgt
    23221 ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa
    23281 agtcgatctt gtcgtacggg ttcaccttct tctccacatc gaggtggaaa ctgttggtgt
    23341 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcagtatcg agacggcaga
    23401 agcggtcgca caggttttca ctcggcgtgc tgtagagact gaaaccataa cccgtttctt
    23461 ctttccactg atccactgcc tgacgcagac gttcaacaat ggcgatacct ttcgcgcgga
    23521 gctgctcgtt gtcgtaaaca tgttcgccgc cgaacagcgc gttaatggtt tcgtggatgc
    23581 cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa atatcatcgt
    23641 cagcattgag acgcacgcca caagcacctt ccatatagag gatcggggcc acgcgcgctt
    23701 tcacgccttc gagacgagcg atacgggtca tcagagcctt acgtgccagc accagacgtt
    23761 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
    23821 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
    23881 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgactt
    23941 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
    24001 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcagacca tcgcgaatcg
    24061 caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct gcgatacggt
    24121 tacgcaggat agattcctga atcaggcgcg actcccagct ggtgcccaga ccaaaaccaa
    24181 aggttacaaa cggcgtctga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg
    24241 actggaaggc atcgtagcac tctttaatgg tacgagagtt agcgtagcct tcggcgtccg
    24301 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggtg acaaacggtg
    24361 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcaacctgag
    24421 caataatctg cgcagttacc gcggttgccg tagagatcga cttcggcggt tcaatctcgg
    24481 cgttccccat cttaaagccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
    24541 acatcgggaa aaacggtgag taatcgagat cgtgatagtg aatatcgcca cgctcatgtg
    24601 cctgcaccac gtcacgcggc agcaggtgct gacgtgcata gtgtttagcc acgatcccgg
    24661 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
    24721 acgaggcgtt ggtctgctcg accagaccac ggatctcctg attcaggcga ccgcgttttt
    24781 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
    24841 gacctgacat cagctggttt tcaacagcgg tctggatctc attgatatcc acctggttgc
    24901 ggccctgcat ctgctcgctg acaactgcag caacagtggc gcaataatcg gcatcatcga
    24961 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
    25021 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
    25081 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
    25141 tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt
    25201 agcaattata gtcgattgat acaacatatt gggttgggac gcattttaaa gtctatatat
    25261 agtgctttgc atcaaggatg tttgaacttt ttttgatgta gctcaaagta aaaagcagag
    25321 cgtacggatg acgggcgcta cagcgttatg taaatttttt aatgaatttg ctggttgaaa
    25381 aatcaacaaa aacaacatgc tgacagacaa aaccccggaa tgaccggcat taccggggct
    25441 tagggaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa gtcatggcac
    25501 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgcgcattt
    25561 gccctggctg ccagggttgg atctcacggc taaggttggc aatgaccagc aaggtttgcc
    25621 ccttccattc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
    25681 tgccccatgt caggatggct tcctgcttac gcagtgcgat taacttttgg taggtgtaaa
    25741 acaccgagga ctcatcggcc agcgcggctt ctacgttgat ttgttgatag ttatcgccca
    25801 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttgctc cattgcatgg
    25861 gcgtgcggct gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
    25921 gctcatcgtt gcgcagctcg gcaaacatat tgaggctctc tacgtcgcga tagtcagtaa
    25981 tgcgcgtgaa atgcgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc
    26041 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
    26101 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattca
    26161 atgctacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt
    26221 caggtttagc cagcgtccat ttttcaccac cgggataatc gaccttcagg tgatgaaaat
    26281 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
    26341 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
    26401 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat
    26461 ccaggtcatc agggaaacgc gggtctttgg agatcagatt cactacatcc aggcgcaacc
    26521 cgtcgacccc acgatcggcc cagaactcac agactttttt cagctctgcg cgtaccgctg
    26581 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
    26641 tttccgcatg ccagcgccac gcactaccgc caaattttga acgccagttg ttcggtggcg
    26701 tttctggttc gccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
    26761 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
    26821 gaatcccgcg cgattttgcc tgcgtcacca gttcgtcaaa atcgtccagc gtgccgtagg
    26881 tgggatcaat cgccgtatag ttcgctacat cgtaaccgtt atcgacctgc ggagagacat
    26941 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
    27001 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    27061 tctggtagat aacgccgttt tgccaccagt ggggaagatt agtcattacg ttattcctgc
    27121 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    27181 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    27241 attgccagcg caaacacctg ccagtagctc ggttgaatcg agagaattcc cggcaggccg
    27301 cctacgccga tgccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    27361 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacattgca
    27421 ggctcagtga ccccaagcca ggcggagatc gccgcaggca cggagatctc gcgttcattg
    27481 tgcttgcggc tggaaatgat aatgcctatc acggcggagc cctgagcgat attcgacagc
    27541 gcaatcagcg gccacactgg cgtaccaccc atgctttgaa tcatctgcaa atcaatagca
    27601 agcgtggtct ggtgtacacc ggtgatcacc agcggggcgt acaggaagcc aaacaatgcc
    27661 gcgccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    27721 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    27781 gaacagacgg gcaccaccac cagatagagg taatccggca cgatgcgttt aaggcgagtt
    27841 tcaataacgc ccagcgccag cccggctaac agtgccggga tcacctgcgc ctgatagccc
    27901 actttggcga tgctgaacat gccaaagtcc cacacttccg gcagctgctg cccgagcaga
    27961 taagcgttca tcagttgggg ggaaaccagt gtcacgccaa gcacgatacc aaggatcggc
    28021 gtgccgccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaagatc
    28081 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    28141 agcgtttgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    28201 ccgccgctaa tcaacgcggg cagcaacggg aagaagatct ctgcgaaatg agagatcaac
    28261 tgctcatgcc atttcatatt ctgccgggcg gcttttttta cctgctcttt atcaacctgc
    28321 gcctgtccgg ttgacgctat cagtgcttga tagtaatcac ccacgttggt gccaatcacc
    28381 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    28441 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    28501 accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg
    28561 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    28621 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta
    28681 tcgttcagga aacgatcagg acaggttggc ggggatgatg atttgttgcg gttcgctgcg
    28741 tccggttacc tgcgcgatca actggcaagc cgcctggcgt ccagcttcgg cgtaaccagg
    28801 atctacggtg acgatctccg gatgaaggaa tttcattaac ggcgtattac cgacgctcgc
    28861 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gtgccagcgt
    28921 gtcggttgcg cacagtaagg cggtagtttc aggcgtaatc acttttgcta cgttctcata
    28981 gccttgcttc atagcaagcc ctggcagggc ggcaacggga tgcagtttat gcgctttgca
    29041 gaacgccagg taggcttcgt gacgtcgctt accggttgtc acgtcactgt gcggcacgcc
    29101 gagataactg atattacgat gcccctggtc atacagccgt tgcatcagga tcttgattgc
    29161 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    29221 cgatgactgc cagtgggcta acatttcttc tgttatgcca gtaaaaccga acagcactac
    29281 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gcggggaaaa
    29341 ctgactttcc atcatgattg ggtcgtaacc ttgttcatag aacgctggca gcatggtttg
    29401 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    29461 gctttgccca cgcatagcgc gcgcagaacg ggaaggggaa aatccatgct gattcatcac
    29521 tgcttcaaca cgctcgcggg tgcgctggct cacgccgctt tcgttattca gcacccggga
    29581 aactgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    29641 catcctgttg tcctgtaacg tgttgtttaa ttatttgagc ctaactttac ccgtgcattc
    29701 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    29761 agatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    29821 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    29881 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa cgtccctgct
    29941 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    30001 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggctcat tcgccattta
    30061 ccttcccgtc tggttcatcg tgatccattg cctggcgcgc agcagacagt gaatacggtg
    30121 gtcccgccgt ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    30181 tggaaaacgt tcgacaccca tccggaaggg ttaaatcagg cggaagtgga atctgcccgc
    30241 gaacaacatg gtgaaaataa attacccgca caacaaccgt cgccgtggtg ggtacattta
    30301 tgggtctgct atcgcaaccc ctttaatatt ttactcacca ttctcggcgc tatttcttac
    30361 gccacggaag atttatttgc cgcgggcgtt atcgcgctaa tggtcgctat ttcaacgttg
    30421 ctgaacttta ttcaggaagc acgttccact aaagcggcag atgccctgaa agcgatggtc
    30481 agcaataccg cgacggtgct gcgcgtaatt aacgacaaag gcgaaaatgg ctggctggag
    30541 atcccgatcg accagctggt gcccggcgat attataaaac tggcggcggg agatatgatc
    30601 ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc
    30661 ggtgagtctc tgcccgtgga aaaagccgct accactcgcc agccggagca cagcaatccg
    30721 ctggagtgcg acacgctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    30781 atggtgattg ctacaggtgc caatacctgg tttggtcaac tggcggggcg tgttagtgag
    30841 caggaaagcg agccgaatgc ctttcagcaa gggatcagcc gcgtcagtat gctgctgatt
    30901 cgctttatgc tggtgatggc accggtggtg ctgttaatca atggctacac caaaggcgac
    30961 tggtgggaag cggcgctgtt tgcgctttcg gtcgcggtag gcctaacgcc ggaaatgttg
    31021 ccgatgattg ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg
    31081 atcgtcaaac atctggatgc tattcagaac tttggcgcaa tggatattct gtgcactgat
    31141 aaaaccggca ccctgacaca ggataaaatt gtgctggaga atcataccga tatctccggt
    31201 aaaaccagcg aacgcgtgct gcatagcgcg tggttgaata gtcattatca gaccggactt
    31261 aaaaacctgc tcgatacagc ggtgctcgaa ggtacggatg aagagtcagc gcgctcgctg
    31321 gccagtcgtt ggcaaaaaat cgatgagatt ccgtttgatt tcgagcgtcg ccggatgtcg
    31381 gtggtagtgg cagaaaatac cgagcaccat cagctggttt gcaaaggtgc attgcaggaa
    31441 atcctcaatg tgtgttcgca ggtgcgtcac aatggcgaga ttgtgccgct cgatgacatc
    31501 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    31561 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    31621 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aaccgctccg
    31681 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    31741 ttagttgcag cgaaagtgtg ccatgaagtg gggctggacg cgggagaggt ggtcattggt
    31801 agtgatattg aaacgctatc tgacgacgaa ctggcaaatc ttgcacagcg cactacgctg
    31861 tttgcccgcc tgacgccgat gcataaagaa cgcattgtga ccttactgaa gcgcgaaggg
    31921 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    31981 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatcctg
    32041 ctggaaaaaa gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc
    32101 aacatgctga aatacatcaa aatgacggcg agttctaact tcggtaatgt gttcagcgtg
    32161 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgttacactt gcttattcag
    32221 aacctgctgt acgatgtgtc acaggtggcg atcccgtttg ataacgtcga cgacgagcaa
    32281 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatgat cttcttcgga
    32341 ccgatcagct cgatcttcga tattctgacg ttttgcctga tgtggtgggt attccatgcc
    32401 aacacgccgg aaacgcaaac gctgttccag tcgggatggt ttgtggtggg cttactgtcg
    32461 caaacgctga ttgtgcatat gatccgcacc cgccgtgtgc cgtttattca gagctgtgca
    32521 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    32581 tcaccgctgg ccagttatct gcaattacag gcgctgccgt taagctattt cccgtggctg
    32641 gttgcgattc tggcagggta tatgacatta acccagttgg tgaaagggtt ctatagccgt
    32701 cgttacggct ggcaataaag aataaactgg gcacgatagc cccgattcag attgcagaca
    32761 aagtaagctt tgttcatgcc ggatgcggcg taaatgcctt atccggccaa caaattcaat
    32821 aaattgcagg aatcacgtag gcctgataag cgtagcgcat caggcgattt tgcgtttgtc
    32881 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    32941 tcgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg
    33001 ccgggaaggt ggcgttgtgt tcggtgaaga aggcttcgta agtggcgttt acggttgcga
    33061 agtcgttcag atcttttaca aacacggtag ttttaacgat gtcgcccact ttcagaccag
    33121 cggcttcgac gatcgctttt acgttatcca gcgactgacg tgcctgtgca gcgacgtctg
    33181 ccggtacttc gcccgttttc ggatttaccg ggatctgacc ggaagtgatg atcatattgc
    33241 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    33301 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    33361 agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa aactcttttt
    33421 cacagtattt gcatttgagc gcgatatcat tggcgcgttt tcgcacggca aagctggaag
    33481 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
    33541 gctccggcag acttgggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg
    33601 tggcttgcgg cgcatacaat gccagttgat ctacctggtc ttcactcaaa aaggtatttt
    33661 cgattttgat cagatctttg cggcccatct cgccagaagg caggttcaga ccgatggtga
    33721 tgcgctgatc cgtttcggtc agcttgaaca gactcaacag cttaaaaccg atctgggcgg
    33781 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    33841 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    33901 ggcgagcgaa aatcccgttg cctgcctgct ggaagtacca ggcgtgtggc gttttatcaa
    33961 catccgtcgc aatctcatca acacgcggca gcggatgaag tactttcata ttggctttgg
    34021 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    34081 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtcaacttcc gccatcactt
    34141 cttcaataga gctgtgcaga ctccatgcga tccctttttc atcaagcata tccagaatgt
    34201 attgcggcat tgccagcgcg tccggcgcga tgaagtaaaa acggttgccg tcgaacttcg
    34261 ctaacgcctg agtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    34321 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    34381 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccggaaaact
    34441 cggtggccag gcgcgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    34501 tgctgataac cgaaatggta tcggccagcg tttcgccctt tttacccagt gatgtattgg
    34561 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    34621 ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc ttcaacagct
    34681 ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    34741 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    34801 tcctgacgcc tgggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    34861 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    34921 actgaaccat acatcctccc ggcaaattgt ccggcattat actcatcgtc agaagcggtg
    34981 caagcatttg atgcaatatt ttgtcagcgc aaacggttta tttgaattat aagtcaaaat
    35041 atatgcattt ttatttactg tgattctgga ggggaactgt cgttcggtat caataattgc
    35101 agacatttcc tgcttttttc tttttcactt tcacgcaatc agatatgcat tttattccat
    35161 tcatccgact tatagggcgg agtgtgaaaa cgaacggcta acactattgc ttacagctca
    35221 gggatgcgcg ctatcacttt aatttcaaaa tcaaagcctg ccagccatgt aacgcccacc
    35281 gccgtccagg ttggataagg tggcgcgcta aatatttcat ttttcaccgt catgatgtct
    35341 tcaaattgtt tttctggatc ggtatgaaag ctcgtaacat caatgatatc gtcaaaagtg
    35401 catcccgcag ctgccagggt cgcatgcaaa ttatcaaatg ccagtctgac ttgttgctga
    35461 aaatcgggtt ctggtgttcc gtcctctcga cttcctactt gcccggaaac aaacagcaaa
    35521 tcgccggaac gaatagccgc agaataacga tgttctgcat acagtgaatg tcggccagca
    35581 gggaaaacag cggttctttc taccatttgg ttatcctcaa gatttacgac atgaacagaa
    35641 gatttatatt taccaggagc cgcttttagc ggacgacgtg agtaaacaaa acccagacat
    35701 catgggaaat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    35761 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    35821 agcgacctct tccggttgcc catgtctttt gatagccata aaactatgca acatatcgcg
    35881 cattggcccg ttggcgggat tagcgtcggt atcaattggc cctggctgga cgacgttgat
    35941 ggtgatccca cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gtagggcaga
    36001 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    36061 gatgattaag atgcgcccac cttcgggcat ctgccgggcg gcttcaacag aggcatggta
    36121 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    36181 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    36241 aacgacatca atgacagcgt ctctgtcagc actatctgta aatactgctg tcgctcccgt
    36301 ctcttgtgcc aggcgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    36361 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    36421 gagaactgtc ttacctgtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    36481 attacaaaac tgttttaact ttctgtcaac aagttttgta atggtcacta aaaaacaatc
    36541 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    36601 gctttttcac caaaaaggtt tcgatgctgt cagtgttgct gaagttactg attatcttgg
    36661 tattaacccc ccgagcctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    36721 actcaatgaa tatgtcggta cggaagctat tccgcttgtc gatattcttc gtgatgatcg
    36781 tccggtaggg gagtgcctgg ctgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    36841 cggcggctgc gctggctgta tggttcttga aggcattcat agtcatgatc cacaagcgcg
    36901 tgatattgcc gttcaatatt atcacgccgc agaaacggcc atttatgact atatcgccag
    36961 gctgcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    37021 ttctgcgaag gcacgagagg ggcactccct cgaacaactc tgtgcaactg ctgcactggc
    37081 gggggaagcg ataaaaacta ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    37141 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggctgaattg
    37201 atcatctttt cgtataacgg tggaaatgaa acattgtttt aattaaggag tggaaaagat
    37261 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    37321 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcacg atatcgaagg
    37381 taatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    37441 ggagtaccat gcccgctatc tcgacattca gattgtgtta aaaggtcagg aaggcatgac
    37501 cttcagcaca caaccggcag gtacgccgga taccgactgg ttagctgata aagacatcgc
    37561 atttttgccg gaaggcgttg atgagaaaac agttatcctt aatgaaggtg attttgttgt
    37621 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggtgcaccag cccaggttcg
    37681 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    37741 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    37801 cttagacgtt cttattaact caaggagttc gtcatgagca aaatatcaga tttgaattat
    37861 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa gtgaagtttt aaatacctgg
    37921 cgtgttggaa tgaataattt tgcccgtaat gccggggggc aggataacac aagaaatatc
    37981 cttaatccta agacattttt ggagtttttg gtaaaaatat ttaccctggg ttatgtggat
    38041 tttagcaaac gctccaacga agcgggaaga aatatgatgg ctcatattga gtcctcatct
    38101 tatatcaaaa ataatgatgg cagtgagata atgaagtttg ttatgaataa tcctgaaggg
    38161 gaacgagcgg attcacccaa ggtgattata gaaatttcac tttccactat tactactatg
    38221 gggactcgtc aaggacatac agccattata tttccacaac ctgatggttc gactaaccgt
    38281 tatgaaagaa agtcctttga aagaaaagat gagagttcat tacacctgat tactaacaag
    38341 gttctggcgt gttaccaacg cgaagctaac aaggaaatag ctcgtctat
//
LOCUS       DAZFBR010000034        38093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFBR010000034 DAZFBR010000000
VERSION     DAZFBR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.034"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(396..1274)
                     /locus_tag="ACHJZ2_001974"
     CDS             complement(396..1274)
                     /locus_tag="ACHJZ2_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4568095.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
     gene            1426..2616
                     /locus_tag="ACHJZ2_001975"
     CDS             1426..2616
                     /locus_tag="ACHJZ2_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4568096.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            2697..3347
                     /gene="inaA"
                     /locus_tag="ACHJZ2_001976"
     CDS             2697..3347
                     /gene="inaA"
                     /locus_tag="ACHJZ2_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO4568097.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(3401..3655)
                     /gene="yfaE"
                     /locus_tag="ACHJZ2_001977"
     CDS             complement(3401..3655)
                     /gene="yfaE"
                     /locus_tag="ACHJZ2_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO4568098.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(3655..4785)
                     /gene="nrdB"
                     /locus_tag="ACHJZ2_001978"
     CDS             complement(3655..4785)
                     /gene="nrdB"
                     /locus_tag="ACHJZ2_001978"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4568099.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(5012..7297)
                     /gene="nrdA"
                     /locus_tag="ACHJZ2_001979"
     CDS             complement(5012..7297)
                     /gene="nrdA"
                     /locus_tag="ACHJZ2_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4568100.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            7425..7568
                     /gene="ypaB"
                     /locus_tag="ACHJZ2_001980"
     CDS             7425..7568
                     /gene="ypaB"
                     /locus_tag="ACHJZ2_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO4568101.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVINNCS
                     AIS"
     gene            7993..11745
                     /gene="yfaL"
                     /locus_tag="ACHJZ2_001981"
     CDS             7993..11745
                     /gene="yfaL"
                     /locus_tag="ACHJZ2_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO4568102.1"
                     /translation="MRVIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVIFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(11886..12608)
                     /gene="ubiG"
                     /locus_tag="ACHJZ2_001982"
     CDS             complement(11886..12608)
                     /gene="ubiG"
                     /locus_tag="ACHJZ2_001982"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO4568103.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            12755..15382
                     /gene="gyrA"
                     /locus_tag="ACHJZ2_001983"
     CDS             12755..15382
                     /gene="gyrA"
                     /locus_tag="ACHJZ2_001983"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO4568104.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            15590..17218
                     /gene="yfaA"
                     /locus_tag="ACHJZ2_001984"
     CDS             15590..17218
                     /gene="yfaA"
                     /locus_tag="ACHJZ2_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO4568105.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWQPL"
     gene            17215..17838
                     /gene="yfaT"
                     /locus_tag="ACHJZ2_001985"
     CDS             17215..17838
                     /gene="yfaT"
                     /locus_tag="ACHJZ2_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO4568106.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            17862..22377
                     /locus_tag="ACHJZ2_001986"
                     /pseudo
     CDS             17862..22377
                     /locus_tag="ACHJZ2_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            22378..24027
                     /gene="yfaQ"
                     /locus_tag="ACHJZ2_001987"
                     /pseudo
     CDS             22378..24027
                     /gene="yfaQ"
                     /locus_tag="ACHJZ2_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            24032..24808
                     /gene="yfaP"
                     /locus_tag="ACHJZ2_001988"
     CDS             24032..24808
                     /gene="yfaP"
                     /locus_tag="ACHJZ2_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO4568107.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            25083..27932
                     /gene="rcsC"
                     /locus_tag="ACHJZ2_001989"
     CDS             25083..27932
                     /gene="rcsC"
                     /locus_tag="ACHJZ2_001989"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO4568108.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(28132..28782)
                     /gene="rcsB"
                     /locus_tag="ACHJZ2_001990"
     CDS             complement(28132..28782)
                     /gene="rcsB"
                     /locus_tag="ACHJZ2_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO4568109.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28799..31471)
                     /gene="rcsD"
                     /locus_tag="ACHJZ2_001991"
     CDS             complement(28799..31471)
                     /gene="rcsD"
                     /locus_tag="ACHJZ2_001991"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO4568110.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            32210..33313
                     /gene="ompC"
                     /locus_tag="ACHJZ2_001992"
     CDS             32210..33313
                     /gene="ompC"
                     /locus_tag="ACHJZ2_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO4568111.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            33425..34480
                     /gene="apbE"
                     /locus_tag="ACHJZ2_001993"
     CDS             33425..34480
                     /gene="apbE"
                     /locus_tag="ACHJZ2_001993"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO4568112.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            34554..35618
                     /gene="ada"
                     /locus_tag="ACHJZ2_001994"
     CDS             34554..35618
                     /gene="ada"
                     /locus_tag="ACHJZ2_001994"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO4568113.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            35618..36268
                     /gene="alkB"
                     /locus_tag="ACHJZ2_001995"
     CDS             35618..36268
                     /gene="alkB"
                     /locus_tag="ACHJZ2_001995"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO4568114.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            36344..37987
                     /gene="yojI"
                     /locus_tag="ACHJZ2_001996"
     CDS             36344..37987
                     /gene="yojI"
                     /locus_tag="ACHJZ2_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO4568115.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         9247 a         9234 c        10215 g         9397 t
ORIGIN      
        1 tttagctcat tttattacga agacctttac gcatgttaga aagtatcatt attgttaata
       61 aatacggtaa taagtaacaa acagatattg ataacttccc tgacaagtta ttaggtatcc
      121 cacttgaaga ttgagaatgg ctccagagta aactgtgtac tgttcgccgg agtgtcatta
      181 ctctcaacaa tcggttttga cagtgagtcg ggcgcttcat accggtttct acctggagtc
      241 attgtcactt ctcccaactg gataaatata tcgccaattc acaaagtggg ccaaaatcag
      301 gtgaaaacga attattacgc ctggtaaagg ctatagtaca actaagtgtg gcgaatcagg
      361 cagtcttgca tttgtcatca atctcatgtc ttacatcaaa tttttccact caccactagt
      421 tgttccgtac acatttgtag taaaaacagt ggactgcctt ctaccggctt ttctttagcc
      481 agcaacgaaa tctgtcgata gaaggttggt tcgagaggaa cggaacacaa ttcatctgca
      541 atggttttca gcgttaactc tggtaataag gcgatgccta atccctgtcg aataaaactg
      601 atcgctgtat ccgggtgatt aaattcatat tttatctgtg gggtaatatt cttttcttta
      661 aatagtgcca taatgcttaa ttcatagcgt cctttactaa tgattaatgg ttcacccatt
      721 aattcttcca ctgtaatagt gctgtttttt gccagagcat ggttgggcgg taacactacg
      781 gtgaatttat cctgataaat gggcacggag tacattccgc tcaccggaaa atgaacaaaa
      841 ccggcatcaa tggattcatt ctgaagcgaa tcaataatcg ctgtgctatt ttcctcaaaa
      901 ggaataattt ttatattcgg atgatgaatt tcaaagtagc gaatcacaac cggtaagata
      961 caggcgcaga cgctggggaa acaaccaagt ctcagcgttc tttgagggtt ctgcttttcc
     1021 tgctcggcta tctcttttac tgcgttaact tcccgattaa tggcgcgcag atgttgcaca
     1081 atgcggtgac ctgctgtggt cagttgtata gctttacggc gatcgcgtaa cagtatattc
     1141 acaccaagtt cttgttcgag ggcagcaatt gcctgactta ccgccgactg cgtcatgaac
     1201 aatcgcttgc tggcttcagt aaagccaccg taatccagca cggcgagtaa agaatttatt
     1261 tgggtaagag tcattagagt ttgctaatca ataatattaa aacgattaat ttctctaata
     1321 gcatgccgcc atttatactt ctgttctgca aagtacatca caatttgacg caaccgcagt
     1381 aacgggagtg ttgtctgcgt gcgtcatcct gacaggcggt agagtatgaa agagaagtta
     1441 tggacgaagg atttttgggc aataaccatc atcagcttta ttattttctt cgtcttttat
     1501 gttttactaa cattgttgcc aatttatatc tctgaccgct tgcatgcctc tcctgataaa
     1561 gcaggtttgt tggtgacttt atttttaatt gcggcgattg ttattcgacc ctttgccggg
     1621 caatgggtgg gtaaatattc gaataaaact attctggtgc tctcttctct ggcctttttg
     1681 gtggtcactg cgctgtatcc tttttgccac tcaatagaat cactgctttt tattagggtg
     1741 cttcatggta ttaccttcgg ggttatcaca acggtaaagg gaacgatttc cgcgcggctg
     1801 atcccggcct cccgacgtgg ggagggcatc agttttttct ctctggcaat ggggctggca
     1861 atggtggtcg ggccgtggat tggcctgaat atggcgcgct gggaggcctt taatatggct
     1921 ttctggttat gtactggcgt tgcggcggtg gggattatcc tgtcgctgat tatgaccgtg
     1981 ccgccggtta tcagccatgc cgacggttca aagccaaaga tgggcttcgc cgccatgttc
     2041 gatcgcgcgg cattgccgtt tgccatggtt acattcttta tgaccttttc gtatgccggg
     2101 gtttctgcct ttctggcgct ttacgcccgc gaacttaatc tgatgtcggc ggccagtaat
     2161 ttcctgctct gctacgccat cttcctgatg atctgccgta ccttcaccgg caatgtttgc
     2221 gacaaaaaag gcccgaaata tgtggtttac ccctgcctgc tgttctttac ggttgggctg
     2281 gtggttctcg gctacaccca gggcagcgta atgatggtcg tttctggcgc gttgattggt
     2341 atcgggtatg gttccgtgac gccagttttt cagacgcaga ttatcagttc agtggaaccg
     2401 cataaaatcg gtgtcgcaaa ctccctcttc ttcaatgcga tggatgcagg cctggcgctg
     2461 ggagcctgtg tgatggggat gatggttgca catactggct accgaatgat ttatctgctg
     2521 ggcgcactat tagtggtagt ggctggtgga gtctatgcgc tgcaaatgaa gggaaaaagc
     2581 ggtgtcgcgc tagtagtggc aaaagaaatt cattaatacg acacgtttca ttaagatttt
     2641 tcgcaggtaa ccacctatag tcattgtcgt taataaaaaa tgacaggcga tagggtatgg
     2701 cagtttcagc aaagcacgac gaatttaatc actggtgggc aacagagggc gactgggttg
     2761 aagagcctaa ctatcgccgt aatggtatga gcggggtgca atgcgtcgag cgcaacggca
     2821 aaaagctata tgtaaagcgc atgacgcatc atctgtttca ttccgtacgt tatcctttcg
     2881 gccgaccaac gattgtccgt gaggttgcgg taattaaaga actggaacgg gccggtgtca
     2941 tcgtgccgaa gatcgttttt ggtgaagcgg tgaaaattga gggggaatgg cgcgcgttgc
     3001 tggtgactga agatatggcg gggttcatca gcattgctga ctggtatgcc cagcatgcag
     3061 taacgcccta ttctgacgaa gtacggcaag ccatgttgaa agcagtggcg ctggcgttta
     3121 agaaaatgca tagcgttaat cgtcagcatg gctgttgtta tgttcgccat atctatgtga
     3181 aaacagaagg taaggcagaa gctggttttc ttgatctgga gaaaagtcgt cgccgcttgc
     3241 gtcgcgataa agcgattaat catgacttcc gccagctgga aaaatatctg gagccgatcc
     3301 ccaaagcgga ctgggagcag gtaaaagcgt attactacgc catgtaatcg ttgtaggcca
     3361 gataatacgc gtaagcgtcg tatctggcgc tagtacccga tcacaactcg atttcaatat
     3421 cgccttttgc tcggcaacaa cagggcaaaa tttcccccgg ctgaataaag gctaacggtt
     3481 cggcaatcca gtccacctgg cctgcaacca ggcgtgtgcg acaggagccg cagtaacctt
     3541 cgcggcactg gtactcaacc gccacattgt gggattccag cgccgccaga agggaagggt
     3601 gttcatcctg gcacagcagt tgtgtgccag tgatgcgcag ggtaacgcga gccatcagag
     3661 ctggaagtta ctcaaatcgt cggtgtccac ttccgagtca atctgcccga ccagataaga
     3721 actgacttcc acttcctgcg gagcaacctg cacgttatca gacaccagcc aggtgttgat
     3781 ccacgggatc gggttggagc gcgtctggaa cggcagatcc aaaccgactg cctgcatgcg
     3841 gatattggtg atgtattcaa cgtactggca gagaatgtct ttattcagac caatcatcga
     3901 accgtcacgg aacagataat ccgcccagtc tttctcttgc tgtgccgcct ggacaaacag
     3961 gtcatagcac tcctgcttac attcttcggc aatttccgcc atctccggat catccgcacc
     4021 gctgcgcagc agattcagca tatgctgagt accggtcagg tgcagggctt cgtcgcgggc
     4081 aatcaggcga ataattttgg cgttaccttc catcagttcg cgttctgcaa aggcaaagga
     4141 acaagcaaag ctgacgtaga agcggatcgc ttccagcgcg ttaacgctca tcaggcagag
     4201 atacagtttt ttcttcagct cgcgcaggct aacggtcacg gttttaccgt taacagtgtg
     4261 ggtgccttcg cccagcaggt gccagtagct ggtcatttcg atcagctcat cgtagtagct
     4321 ggagatccct tccgcacgtt tctggatctg ctcgttggtg acgatatcgt caaacacaac
     4381 agacggatcg ttaacgatat tacgaatgat atgagtatag gaacgggaat gaatcgtttc
     4441 tgagaacgcc caggtttcta cccaggtttc cagttccggg atcgagatca gcggcagcag
     4501 tgccacgttc gggctacgac cctgaatgga gtccagcagc gtctgatatt tcaggttgct
     4561 gataaagatg tgtttttcgt gttcaggcag cgcctggtaa tcgatacggt cacgagaaac
     4621 gtcaacttct tccggacgcc agaagaaaga gagctgcttt tcgatcagct tttcgaagat
     4681 gtcatatttt tgctgatcgt aacgggccac gttgaccggc tgaccaaaga acatcggttc
     4741 tttgagctga tcatttttcg tctgtgaaaa ggtggtatat gccatgagtg tgtcctgttg
     4801 ggagtttaat gccggataag gcattttacg ccgcatcctg tgcctgatgc gacgctggcg
     4861 cgtcttatca ggcctacaaa tccgagccgt aggccggatt aggcgttcac gccgcatcct
     4921 gtgcctgatg cgacgctggc gcgtcttatc aggcctacaa accgagccgt aggccggata
     4981 aggcgtttac gccgcatccg gcatctcaat atcagatctt acatgcgccg ctttcgcagc
     5041 catcgtcctg gattgacggc actagatcgt cctgcgcgtc ttcagcgccg tcacgggtgt
     5101 tctgataata cagcgttttg accccgaatt tgtaggcggt gagcaggtct ttcagcaact
     5161 gctgcatcgg cacttttcct gacgggaagc gtgacggatc gtagttggtg ttagcagaga
     5221 tcgactgatc gataaatttc tgcatgatac ccaccagttg caggtagcca tcgttacccg
     5281 gcatttccca cagcagctca taggcgtcgt gcagatgctc gtagtccggc accacctggc
     5341 gcagaatacc gtctttcgac gctttgatgc tgacgtaacc gcgcggcggt tcaataccgt
     5401 tagtggcgtt agagatctgc gaagaagtct cggacggcat cagagcagaa agcgtggagt
     5461 tacgcaggcc gtgtgttttg attgactcac gcagagcttc ccagtcgtaa tgcagcggtt
     5521 cattagcgat ggcatccaga tctttcttat aggtatcgat cggcaggatc cctttcgcgt
     5581 aagtggtttc gttaaaccac gggcacgcgc cttgctcttt cgccagctca ttagacgctt
     5641 tcagcaggta atactgaatc gcttcgaagg ttttatgcgt caggttgttg gcgctgccgt
     5701 cggagtagcg tttaccatgc ttcgccaggt agtaagcgaa gttgatcaca ccaataccca
     5761 gcgtacgacg acccatcgct ccacgtttgg cggccgggat cgggtaatcc tgataatcca
     5821 gcagcgcgtc aagtgcacga accgccagaa ttgccagctc ttccagttca tccaggctat
     5881 taattgcgcc caggttgaaa gcagacagcg tacacagcgc gatttcaccg ttctcgtcgt
     5941 tgacgtcgtt cagcggtttg gtcggcaggg ctatctccag gcacaggtta gactgacgca
     6001 ctggcgcgat ggccggatca aacgggctat gggtattgca gtggtcaacg ttctgaatat
     6061 agatacgacc ggtagacgca cgttcctgca tcatcagcga gaacagctca acggctttca
     6121 cacgctgctt gcggatgctg tcgtctttct catatttggt atacaggcgt tcaaactctt
     6181 cctgatcggc gaagaatgcg tcgtacagcc ccggtacgtc ggacgggctg aacagggtga
     6241 tatcttcacc tttcagcagg cgggtataca tcagtttgtt gatttgtacc ccgtagtcca
     6301 tatgacgcac gcggttgcct tccacaccac ggttgttttt caacaccagc aggctttcca
     6361 cttccagatg ccacatcggg tagaacagcg ttgccgcacc gccgcgcacg ccgccctgag
     6421 agcaggattt caccgcagtc tggaaatgtt tgtagaacgg aatgcagccg gtatggaacg
     6481 cttcgccacc gcgaatcggg ctacccagcg cacgaatacg cccggcgttg atgccgatcc
     6541 cggcacgctg ggaaacatat ttcacaatcg cgctggaggt ggcgttgatg gaatccaggc
     6601 tgtcgccgca ctcgatcagt acgcaggagc tgaactgacg agtcggggta cgcacgccgg
     6661 acatgattgg cgtcggcagc gaaattttaa atgtggaaac cgcgtcgtaa aaacgcttca
     6721 catattgcag gcgcgtttca cgcgggtagt tcgagaacag gcacgcagca accagaatat
     6781 aaaggaactg ggcgctttcg tagatttcgc cggtcacacg gttctgtacc agatatttgc
     6841 cttccagttg cttaacggca gcataagaga aggtcatatc acggtcgtga tcgataaacg
     6901 tgtccatctg cttgaactct tcttccgtgt agtcttccag cagatgatta tcgtatttgc
     6961 ccatctcgac cattttcacc acgtggtcgt acagcgcagg cggctcaaac tggccgtagg
     7021 cttttttacg caggtggaag atcgccaggc gcgcggcgag atactgataa tccggcgcat
     7081 cacgggagat caggtctgcg gcagccttga taatggtttc gtggatgtca gaggtcttga
     7141 taccgtcata aaactgaatg tgggagcgca gctcgacctg ggaaatcgaa acgttatgca
     7201 gtccttctgc cgcccaatcc agaacgcgat ggattttgtc gagattgatg cgctctgtgc
     7261 taccgtcgcg ctttgtcacc agcagattct gattcatgta tgtcgtacct gtttttggaa
     7321 ttctttccgc aatagtttcg tgcagactac ctgtagtacg ggaagctgct tagaacacta
     7381 tatatagggg gtatgtttgg gattcactgc aagatagtgt gaaaatggcc ctcttgcaag
     7441 tgcataactt tgtggataac tcaggaagga aaaagtggct ttcgcgcacc ttaggtcaga
     7501 caaggtgtcc gggaaagtca atgggaagag aaaaatttgt tataaataac tgttcggcga
     7561 tttcttgaac ggcagaatag gttggataaa ttgatataga tggtttatta atctttttgc
     7621 taatgattgc aaaaattgat ctaagcacga gtcatcacat aatctgatgt atcagttgtg
     7681 gtcaatatat aagcgaatgt aagcgattgt tgatgtcgaa atggttgttt tctattcggt
     7741 tattgttaaa tgcgaagcca ttttgttaat ttgccttata ttataagaaa attattcatt
     7801 actttaattt agtttaaata taactggtat cccttaaggt tattaacttg aattatgacg
     7861 cactgatact attcatcaaa taataacaaa atagctattg catcgggttg gacaatttac
     7921 aaaagaaaga taacccatat tgtataatgc gtttaaatat gccgtcttat ataaaatgat
     7981 ggtcagatta atatgcgggt tatctttcta cgcaaggagt atttatctct actcccgtca
     8041 atgattgcat ctcttttctc tgctaacggt gtcgcggcgg ccattgattt atgccaggga
     8101 tatgatatca aagcgagttg tcacgccagc aggcaaagcc tttcaggcat tacgcaggtc
     8161 tggagtattg ccgatgggca atggctggtt ttttcggata tgaccaataa tgccagcggt
     8221 ggggccgtat ttttgcaaca aggagcggaa tttacattat caccagaaaa tgaaactgga
     8281 atgactctgt ttgccaataa caccgtttca ggagaatata ataacggcgg ggcaatattt
     8341 gctaaagaaa actcaacgct gaatcttacg gatgttattt tttctggtaa cgtcgcaggc
     8401 ggctatggtg gcgcaatcta ttcttctggt actaacgata ccggtgccat cgatttacgt
     8461 gtcactaacg ccgtgtttcg caataacatc gctaatgacg gcaaaggtgg tgcaatttat
     8521 accatcaata atgatatcta tttaagtgat gatgttttta acaataacca ggcatataca
     8581 tcaacaagtt acagtgatgg cgatggcggc gcaatcgatg tcacagataa taatagcgac
     8641 agcaagcatc cttcaggtta tacgataata aataacactg cctttacaaa taacactgcc
     8701 gaaggttatg gcggggcgat atataccaat agcgcgacgg ctccctatct tattgatatt
     8761 tctgttgatg acagctacag ccagaacgga ggcgtgttag tcgatgagaa caatagcgca
     8821 gcaggctatg gagatggtcc ttcctctgcg gcgggtggct ttatgtatct cggcttaagt
     8881 gaagttatct ttgatattgc cgacggaaaa acgctggtta ttggcaatac agagaatgac
     8941 ggagctgttg actctattgc tggtaccggg ttaatcacca aaacaggttc cggcgatctg
     9001 gtacttaatg cagataacaa tgactttact ggtgagatgc agattgaaaa cggtgaagtt
     9061 accctgggcc gcagcaactc cctgatgaat gtcggcgata cgcattgcca ggacgatccg
     9121 caagactgct acggtctgac gatagggagt attgataagt accagaatca ggcagagcta
     9181 aatgttggct ccacccaaca aacctttgcg cactcattga cgggctttca gaatggcact
     9241 ttaaatatcg atgctggtgg caatgttact gttaatcaag gcagttttgc tggcaccatc
     9301 gaaggtgctg gtcagctcac cattgcgcaa aacggcagct atgtgctggc gggggcgcag
     9361 tcgatggcgc taaccggcga tatagtggtg gatgctggtg cggtgctttc gctggaaggc
     9421 gacgcggcag atcttgccgc tctccaggac gatccgcagt cgatcgtgtt aaacggcggt
     9481 atgctcgatc tctctgattt ctccacctgg cagagcggta catcatacaa agatggcctt
     9541 gaagtcagtg gcagcagcgg aacggttatc ggcagtcagg atgtggtaga tcttgcaggc
     9601 ggaaacgata tgcatatcgg cggcgacggg aaagatggcg tctacgtggt gatcgatgcg
     9661 ggtgacgggc aggtcagcct ggcaaatgac aatcaatacc tcggcacaac gcaaatcgct
     9721 tccggtacgc tgatggtgag cgacaactcg cagcttggat atacccatta taaccgccag
     9781 gttatcttta ccgataagcc acaagaaagc gtgatggaga ttactgccaa tgtcgatact
     9841 cgctctacaa cgactgagca tgggcgtgat attgaaatgc gcgccgacgg tgaagtggca
     9901 gttgatgcgg gggtagacac gcagtggggc gcactgatgg ctgacagcag cgggcagcat
     9961 caggatgagg gtagcacatt gactaaaacg ggggcgggta cactggagct gaccgccagc
    10021 ggtacaacgc agtcagcggt gagagtagaa gagggcacgc tgcaaggtga tgttgcggat
    10081 atcttccctt atgcttcgtc gctatgggtc ggtgacgggg caacgttcgt tactggcgcg
    10141 gatcaggata ttcagtcaat tgatgctact tccagcggca ctatcgacat cagcgatggt
    10201 acggttttgc gcctgaccgg gcaggatact tccgtcgccc ttaatgcctc actgtttaac
    10261 tgcgatggga cgctggtgaa tgccaccgat ggtgtgacgt tgacaggtga gcttaatacc
    10321 aaccttgaaa ctgacagcct gacttatctt tccaacgtga cggttaatgg caatctgacc
    10381 aatacgtccg gtgcggttag cctgcaaaat ggcgtcgctg gcgatacgct gacggtaaac
    10441 ggtgattata ccggcggcgg tacgctactg ctcgatagcg aattaaacgg cgatgactcg
    10501 gtaagcgatc aattggtgat gaacggtaat actgctggca acacaactgt ggtggttaac
    10561 tccattacag ggattggtga gccgacatcg acaggcatta aagtggttga tttcgcagct
    10621 gatcccacgc agtttcaaaa caatgcgcag ttcagtctgg caggcagcgg ctacgtcaat
    10681 atgggagcgt atgactacac gctggtggaa gataacaacg actggtatct gcgatcgcaa
    10741 gaagtaacgc cgccatcgcc acctgatcca gacccgactc ccgatcctga tcccacgcag
    10801 gatcctgatc caacacccga cccggaacct acgcctgctt accagccggt gttgaatgcc
    10861 aaagttggcg gttatctcaa taacctgcgg gcggcaaatc aggcgtttat gatggagcga
    10921 cgcgatcacg caggtggcga tggtcagacg ctgaatttac gtgttatcgg cggagattat
    10981 cattacacag cagcggggca actggctcaa catgaagaca cttctacggt gcaacttagc
    11041 ggcgatctgt ttagcgggcg ctggggcacg gatggcgagt ggatgcttgg gattgttggt
    11101 ggctacagcg ataaccaggg cgacagccgc tcgagtatga ccggaactcg cgccgataac
    11161 cagaaccacg gttatgcggt tgggctgacc tcaagctggt ttcagcacgg taagcagaag
    11221 cagggggcct ggctggataa ctggttgcag tacgcgtggt ttagcaatga tgtttctgaa
    11281 catgaagatg gcgtggatca ttaccattcg tcggggatta tcgcctcgct ggaagcgggg
    11341 tatcagtggt taccggggcg tggtgtggtg attgaaccgc aggcgcaggt gatttatcag
    11401 ggcgtgcagc aggatgattt taccgccgct aaccgtgcgc gcgtgtcaca atcgcagggt
    11461 gatgatattc agacgcggct gggtttacac agcgaatggc gtaccgctgt tcatgtcata
    11521 ccaacattag atctgaatta ttatcacgat ccccattcga cggaaattga agaagatgcc
    11581 agcactatca gtgacgatgc ggtgaagcaa cggggtgaaa taaaagtggg agtcacgggc
    11641 aatatcagtc agcgagtttc gctgcgtggt agcgtggcgt ggcagaaagg gagtgatgat
    11701 tttgcccaga cggcagggtt tttgtcgatg acggtgaaat ggtaatcgct gtctgaccat
    11761 cactattgct ggatgcggcg taaacgccta atcaggccta cggagagcat ttgtaggcct
    11821 gataagacgc ggcaagcgtc gcatcaggca ttggcgcggt tgccggatga atccaaacaa
    11881 caacctcact tattctgcgt gtgcagcata tagttcacat ccacgccggg gccgagttta
    11941 aaagtattag tgatcgggtt gtaatgtagc ccggttatat gtcgctcttt aaggcttgtc
    12001 tgatctaccc agcccagcag ttctgccggt ttaataaact tcttcacatc atgtgtacct
    12061 ttaggcacca tgcgcaaaat atattccgcg ccgactaccg ccatcagcca tgacttgccg
    12121 ttgcggttaa gcgtcgagaa aaagacatcg ccgcccggtt tcaccagttg cgcacaggct
    12181 ctgaccactg actgcggatc agggacgtgc tccagcatct ccatgcaggt caccacatca
    12241 tactgcccgg catgttttgc cgcgtgctct tccacggttt cctgcacgta atccacctga
    12301 atgccgcttt ccagcgcgtg cagctttgcc acctgcaatg gctcaaagcc catatccaga
    12361 ccggtcaccg tcgcgccttc gcgcgccata ctctcggcca gaatgccgcc gccacaaccg
    12421 acatcgagca cctttttgcc aaataagccg ccagcacgct cggcaatata gctcagacgc
    12481 agcgggttaa tgcggtgcag cggtttgaac tcaccttcca gatcccacca gcgggaggcg
    12541 acggcttcaa atttagcgat ctcttcgtgg tctacgttat ggtttaccgg cgatttttcg
    12601 gcattcattg gcacttctac tccgtaattg gcaagacaaa cgagtatatc aggcattgga
    12661 tgtgaataaa gcgtataggt ttacctcaat ctgcgcggct gtgttataat ttgcgacctt
    12721 tgaatccggg atacagtaga gggatagcgg ttagatgagc gaccttgcga gagaaattac
    12781 accggtcaac attgaggaag agctgaagag ctcctatctg gattatgcga tgtcggtcat
    12841 tgttggccgt gcgctgccag atgtccgaga tggcctgaag ccggtacacc gtcgcgtact
    12901 ttacgccatg aacgtactag gcaatgactg gaacaaagcc tataaaaaat ctgcccgtgt
    12961 cgttggtgac gtaatcggta aataccatcc ccatggtgac tcggcagttt atgacacgat
    13021 cgtccgtatg gcgcagccat tctcgctgcg ttacatgctg gtagacggtc agggtaactt
    13081 cggttccatc gacggcgact ctgcggcggc aatgcgttat acggaaatcc gtctggcgaa
    13141 aattgcccat gaactgatgg ccgatctcga aaaagagacg gtcgatttcg ttgataacta
    13201 tgacggcacg gaaaaaattc ccgacgtcat gccaaccaaa attcctaacc tgctggtgaa
    13261 cggttcttcc ggtatcgccg taggtatggc aaccaacatc ccgccgcaca acctgacgga
    13321 agtcatcaac ggttgtctgg cgtatatcga tgatgaagac atcagcattg aagggctgat
    13381 ggaacacatc ccggggccgg acttcccgac ggcggcaatc attaacggtc gtcgcggtat
    13441 tgaagaagct taccgtaccg gtcgcggcaa ggtatatatc cgcgcccgtg ctgaagtgga
    13501 agttgacgcc aaaaccggac gtgaaaccat tatcgtccac gaaattccgt atcaggtaaa
    13561 caaagcgcgc ctgatcgaga agattgcgga actggtaaaa gaaaaacgcg tggaaggcat
    13621 cagcgcgctg cgtgacgagt ctgacaaaga cggtatgcgc atcgtgattg aagtgaaacg
    13681 cgatgcggtc ggtgaagttg tgctcaacaa cctctactcc cagacccagt tgcaggtttc
    13741 tttcggtatc aacatggtgg cattgcacca tggtcagcca aagatcatga acctgaaaga
    13801 catcatcgcg gcgtttgttc gtcaccgccg tgaagtggtg acccgtcgta ctattttcga
    13861 actgcgtaaa gctcgcgatc gtgctcatat ccttgaagca ttagccgtgg cgctggcgaa
    13921 catcgacccg atcatcgaac tgatccgtca tgcgccgacg cctgcagaag cgaaaactgc
    13981 gctggtggct aatccgtggc agctgggcaa cgttgccgcg atgctcgaac gtgctggcga
    14041 cgatgctgcg cgtccggaat ggctggagcc agagttcggc gtgcgtgatg gtctgtacta
    14101 cctgaccgaa cagcaagctc aggcaattct ggatctgcgt ttgcagaaac tgaccggcct
    14161 tgagcacgaa aaactgctcg acgaatacaa agagctgctg gatcagatcg cggaactgct
    14221 gcgtattctt ggtagcgccg atcgtctgat ggaagtgatc cgtgaagagc tggagctggt
    14281 tcgtgaacag ttcggtgaca aacgtcgtac tgaaatcacc gccaacagcg cagacatcaa
    14341 cctggaagat ctgatcaccc aggaagatgt ggtcgtgacg ctctctcacc agggctacgt
    14401 taaatatcag ccgctttctg aatacgaagc gcagcgtcgt ggcgggaaag gtaaatctgc
    14461 cgcacgtatt aaagaagaag actttatcga ccgactgctg gtggcgaaca cgcacgacca
    14521 tattctgtgc ttctccagcc gtggtcgcgt ctattcgatg aaagtttacc agttgccgga
    14581 ggccactcgt ggcgcgcgcg gtcgtccgat cgtcaacctg ctgccgctgg agcaggacga
    14641 acgtatcacc gcgatcctgc cggtgaccga gtttgaagaa ggcgtgaaag tcttcatggc
    14701 gaccgctaac ggtactgtga agaaaaccgt cctcaccgag ttcaaccgtc tgcgtaccgc
    14761 cggtaaagtg gcgatcaaac tggttgaagg cgatgagctg atcggcgttg acctgaccag
    14821 cggcgaagac gaagtaatgc tgttctccgc cgaaggtaaa gtggtgcgct ttaaagagtc
    14881 ttctgtccgt gcgatgggct gcaacaccac cggtgttcgc ggtattcgtt taggcgaagg
    14941 tgataaagtc gtctctctga tcgtgcctcg tggcgatggc gcaatcctca ccgcaacgca
    15001 gaacggttat ggtaaacgta ccgcagtggc ggaatacccg actaagtcgc gtgcgacgaa
    15061 aggggttatc tccatcaagg ttaccgaacg taacggttta gttgttggcg cggtacaggt
    15121 agatgactgc gaccagatca tgatgatcac cgatgccggt acgctggtac gtactcgcgt
    15181 ttcagaaatc agcatcgtgg gccgtaacac ccagggcgtg atcctcatcc gtactgcgga
    15241 agatgaaaac gtagtgggtc tgcaacgtgt tgctgaaccg gttgacgagg aagatctgga
    15301 taccatcgac ggcagtgccg cggaagggga cgatgaaatc gctccggaag tggacgttga
    15361 cgacgagcca gaagaagaat aattttactt cttcatgcca aaagggagcc atctcccttt
    15421 tttgaattga aaagtccagg ctgcaaagtc tgggcttttg tcgtattagg gcgcggtaaa
    15481 gtttggctgt gtccgtaaaa aatggctggc tatacacaag gaatgtggca atgagtggtg
    15541 aaaaaaagcg aaaggctggc ggttctatgg tcttgtaggt tttggcgcaa tagcactgct
    15601 ttccgctggc gtctgggcgt tgcaatatgc tggcagtggg ccagaaaaaa cgttgtcgcc
    15661 gctggtggtg cacaataatt tgcaaattga tctcaatgag ccggacctct ttctcgacag
    15721 cgattctcta agccagcttc ccaaagatct cctcaccatt ccgtttctcc acgatgttct
    15781 gagtgaagat ttcgttttct attatcagaa tcatgccgat cgtctgggca ttgaaggcag
    15841 cattcgtcgc attgtctatg aacacgatct cacgctgaaa gataagctct tttcgtcact
    15901 cttagatcag cccgcgcagg cggcgctatg gcacgataaa caaggccatc tttcacatta
    15961 catggtgctg atccaacgca gtggtttaag caaactgctg gagccattgt tatttgccgc
    16021 taccagcgac agccagttaa gcaaaacgga aatcagtagc atcaagataa atagtgaaac
    16081 tattcctgtt tatcagttgc gctataacgg caataacgcc ctgatgttcg cgacatatca
    16141 ggacaagatg ctggtgtttt ccagcacgga tatgctgttt aaagatgatc agcaggatac
    16201 cgaagccacg gcgatcgcaa gtgatttgtt gagcggcaaa aaacgctggc aagccagctt
    16261 tggcctggaa gagcgtgctg ccgaaaaaac gccagtacgc cagcgcatcg tggccagcgc
    16321 caggttgctg ggttttggct accagcggtt aatgccttct tttgctggcg tacgcttcga
    16381 aatgggtaac gacggctggc acagttttgt ggcgttaaat gatgaatccg ccagcgtaga
    16441 tgccagtttc gattttacgc cggtctggaa cagtatgcct gccggagcca gcttctgtgt
    16501 ggcggtgccg tattcacacg gtattgccga agagatgctt tcgcacatca gccaggaaaa
    16561 cgacaagttg aatggggcgt tggacggtgc cgcggggctg tgctggtatg aagactcaaa
    16621 attgcaaacc ccgctgtttg tcggtcagtt tgatggcact gccgaacagg cgcaattgcc
    16681 agggaaactg tttacgcaaa atattggtgc gcacgaaagc aaagcgccag aaggtgtttt
    16741 gccggtaagc cagactcagc agggcgaagc gcaaatctgg cgtcgcgaag tgagttcccg
    16801 atacggtcag tatccgaaag cgcaggcggc gcaacccgat caattaatgt cggattattt
    16861 tttccgcgtg tcgctggcga tgcaaaacaa aacgctgctt ttctccctcg atgacacgct
    16921 ggttaataac gccctgcaaa cactgaataa aacccgcccg gcaatggtgg atgtaatacc
    16981 cactgatggc atcgttccgc tctatatcaa tccacaaggc gtggcgaaac tgctgcgtaa
    17041 cgaaacgttg accagtctgc cgaagaatct cgaaccggtt ttttataacg ccgcacaaac
    17101 tttattaatg ccgaagctgg acgctttatc tcaacaaccg cgttatgtca tgaagctggc
    17161 ccagatggaa cccggtgccg cctggcagtg gctacccata atctggcagc cgctatgagg
    17221 cacgggctgc tggcgctgat ttgctggctg tgttgtgttg ttgcccatag cgaaatgctg
    17281 aatgtcgaac aatccgggct gtttcgcgcc tggtttgtac gcattgcaca agaacagctc
    17341 cgccaggggc caagtccacg ctggtatcag caggattgtg cgggcctggt gcgatttgcg
    17401 gcgaacgaga cgctgaaagt tcacgacagt aaatggctaa aaagtaacgg tttatctagc
    17461 cagtatttgc cgccagagat gacgctaaca cctgaacagc gtcaactggc gcaaaactgg
    17521 aatcagggga acgggaaaac cggcccctac gtgaccgcga ttaatttgat tcagtacaac
    17581 agccagttta ttggccagga cataaaccag gcgctgcctg gcgatatgat ttttttcgat
    17641 cagggcgatg cccagcactt aatggtctgg atggggcgtt acgtcatcta ccacaccgga
    17701 agcgccacga aaactgacaa cggaatgcgc gcagtcagtc tgcaacaact tatgacatgg
    17761 aaggacaccc gatggatacc caacgattcc aatcccaatt tcattggcat ttatcgttta
    17821 aattttctgg cgcgatagca gcgtgcttgt ccctgtctct tgtgggaaca gggcttgcta
    17881 atgctgatga ttcgcttcct tccagtaact atgcgccgcc cgccggggga acattctttt
    17941 tgcttgctga cagcagtttt agcagcagtg aagagtcgaa agtgcgactg gaagcgccgg
    18001 ggcgtgatta tcggcgctat cagatggaag agtacggcgg cgtggacgtt cgcctgtatc
    18061 gtattcctga cccgatggca tttttgcgcc agcagaaaaa cctgcatcgc attgtggtgc
    18121 aaccgcaata tctgggcgac gggctgaaca atacgctgac ctggctgtgg gataactggt
    18181 acggcaaatc tcgccgcgtg atgcagcgta ctttctcttc tcagtcacgg cagaatgtga
    18241 ctcaggcatt acccgaatta cagctcggca atgccattat taaaccttcc cgttatgtac
    18301 agaacaacca gttttccccg ctgaaaaaat atcccctggt ggaacagttc cgttatccac
    18361 tatggcaggc taaaccggtc gagccgcagc aaggggtaaa actggaaggc gcatccagca
    18421 atttcatctc gccgcagccg ggtaacattt atattcctct cggccaacaa gagccgggac
    18481 tgtacctcgt cgaggcgatg gttggtgggt atcgggcgac gacggtggtg tttgtttccg
    18541 ataccgtggc gcttagcaaa gtgtcaggca aagagcttct ggtgtggacc gcgggtaaaa
    18601 aacagggtga agcgaagccc ggctcagaga tcttgtggac tgacggtctt ggcgtgatga
    18661 cccgcggtgt gaccgatgac agcggtacct tgcagttaca acatatatcg ccagaacgtt
    18721 catacattct gggtaaggat gctgaaggcg gcgtttttgt ctccgagaac ttcttctacg
    18781 aaagcgaaat ctacaacacc cgcttgtata tttttaccga tcgcccgcta tatcgcgcag
    18841 gcgatcgtgt cgatgttaaa gtgatgggcc gcgagttcca cgatccgttg cattcatccc
    18901 ccatcgtcag cgccccggcg aagctttcgg tgctggacgc taacggcagt ctgttgcaaa
    18961 ccgtcgatgt cacgctggat gcgcgcaatg gcgggcaggg aagtttccgc ctgccagaaa
    19021 atgccgttgc cggaggttat gagttacgtc ttgcttaccg caatcaggtc tatagcagca
    19081 gttttcgcgt ggcaaactac atcaagccac atttcgagat tggtttagct ctcgacaaaa
    19141 aagagttcaa aactggcgaa gcggtcagcg gcaaactgca acttctctac ccggatggcg
    19201 agccggtaaa aaatgcccgc gtgcagttaa gtttgcgcgc tcagcaatta tcaatggtcg
    19261 gtaacgattt gcgttatgcc ggacgtttcc ccgtgtcgct ggaaggcagc gaaacggtgt
    19321 ccgacgccag cggtcatgtg gcgttaaatc tccccgccgc cgataaaccg agccgctatt
    19381 tgttaaccgt ctccgccagt gacggcgcgg cgtatcgcgt caccaccacc aaagagatcc
    19441 tcattgaacg cggcctggcg cattactctt taagtaccgc cgcacaatac agtaatagcg
    19501 gcgagtcggt tgtgttccgt tatgccgcgc tggaatcttc aaaacaggtt cctgttacgt
    19561 atgaatggtt gcgtctcgaa gaccgcacga gccatagcgg agatctaccg tcaggcggca
    19621 aatcctttac cgtcaatttc gataaacctg gcaactacaa tctgacgtta cgcgataaag
    19681 acggcttaat tctcgccggg ttaagccatg ccgtcagcgg taagggcagt atgtcgcata
    19741 ctggtacggt agatatcgtg gcagataaaa cgctgtacca gcctggcgaa accgcgaaga
    19801 tgctgattac cttcccggag ccaattgatg aagcattatt gacgctggaa cgcgatcgcg
    19861 ttgaacagca gtcgctgcta tcgcatccgg caaactggct gacgttacaa cgtttaaacg
    19921 atactcaata tgaagcccgt gttccagtga gcaattcctt tgcgcctaac atcacttttt
    19981 cggtgctgta tacccgtaac ggtcagtaca gttttcagaa cgccgggatc aaagttgccg
    20041 ttccccagct ggatatccgg gtgaaaacgg acaaaaccca ttaccagcct ggtgaactgg
    20101 tcaatgtcga attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag
    20161 gcgtggtcga tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat
    20221 ttttctatcc gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct
    20281 acgaccaggc actctccagc gagccggttg cgcctggcgc aactaaccgc agcgagcggc
    20341 gagtaaaaat gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt
    20401 cactcacaac cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa
    20461 cccgctggcg tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg
    20521 cttatctgcg ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg
    20581 tgggcgacaa accgtcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg
    20641 cgctggtgac taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg
    20701 cgaattatat ttcgctggcg cagaacattc agcaatctgg cttgttaagc gcagaactgc
    20761 aacaaaatgg gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gataacagct
    20821 ggcccgttga acagcagaaa aatgtcatgc tcggcggtgg cgataacgcg ctgatgttgc
    20881 ccgagcaggc gagcaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca
    20941 acaatcttga tgcgttagtc gatgaaccgt gggggggggg tgatcaacac cggtagccgt
    21001 ctgatcccgc tcagtctcgc ctggcgttcg cttgccgatc atcaaagtgc cgccgctaac
    21061 gacattcgtc agatgattca ggataaccgt ctgcggctga tgcaactggc ggggcccgga
    21121 gcgcgcttta cctggtgggg tgaagatggc aatggtgacg ccttccttac ggcatgggca
    21181 tggtacgccg actggcaggc cagccaggcg ctcggcgtaa cgcaacaacc ggaatactgg
    21241 cagcatatgc tcgacagtta tgccgagcag gcagataaca tgccgttatt gcatcgggcg
    21301 ctggtgctgg cgtgggcaca ggagatgaat ctgccgtgca aaacgttgtt gaaagggttg
    21361 gatgaagcta tcgcccggcg cggaactaaa actgaagatt tctctgagga agacacccgc
    21421 gatatcaatg acagcctgat cctcgataca ccggaatctc cactggcaga tgcggtggca
    21481 aacgtcttaa ccatgacgtt gctgaaaaaa gcgcagttga agtccacggt gatgccacag
    21541 gttcagcaat atgcgtggga taaagcggta aacagcaatc agccgctggc acacacggtt
    21601 gtgctgctca atagcggggg cgacgctacc caggcggctg ctattttaag tggtttgacc
    21661 gctgagcaat ctactattga gcgcgcgctg gccatgaact ggctggcgaa atatatggcg
    21721 acaatgcctt cggttgtgtt gcctgcgcct gcgggcgcat gggccaaaca taagttaact
    21781 ggagggggcg aatactggcg ttgggttggt cagggcgtgc cggacattct ctcttttggt
    21841 gacgaattat cgccgcaaaa tgtgcaggtc cgctggcgtg aagcggcaaa aacggctcaa
    21901 caaagtaaca ttccggtgac cgttgaacgc caattgtatc ggcttatccc tggtgaagaa
    21961 gagatgagct ttactctgca accggtgacc agcaatgaga ttgacagcga tgcgctgtat
    22021 ctcgatgaaa ttacgcttac cagcgagcag gatgcagttc tgcgctatgg tcaggtggaa
    22081 gtaccgctcc cgccgggagc cgacgttgag cgcacaacat ggggcatttc agtcaataaa
    22141 cccaacgctg gaaaacagca ggggcaattg ctggaaaaag cgcgaaatga aatgggcgaa
    22201 ctggcctata tggtgccggt gaaagaactg acgggaacgg tcactttccg ccatttgctg
    22261 cgcttctcgc aaaaagggca attcgttctg cctcctgctc gttatgtgcg ttcctatgca
    22321 cctgcacagc aaagtgttgc ggcagggagc gaatggaccg ggatgcaggt gaaataagtg
    22381 aactggcgca gaatcgtctg gctattggcg ttggttactc tgccaacgct tgcggaggag
    22441 acgccgttac aactggcgct gcgcggtgcg cagcacgatc aactgtatca gctgtcgtca
    22501 tcaggcgtga ccaaagtctc agcattacct gacacgctca ccacgccgtt agggagttta
    22561 tggaaactgt atgtttacgc ctggctggag gatacccatc aaccggagca gccctaccag
    22621 tgtcgcggaa actccccgga agaggtttat tgttgtcagg ctggggagag cataacgcgc
    22681 gataccgcgc tggtacgttc ctgtgggctc tattttgcgc cgcagcggtt gcatattggg
    22741 gcagatgtgt gggggcaata ctggcaacag cgtcaggcac ctgcgtggtt agcgtctctt
    22801 tctacgctga agccagaaac ctcggtaacg gttaaatcgc tgcttgattc attagcgacg
    22861 ttacctgcgc aaaacaaagc tcaggaagtc ttactggatg tggtgctgga cgaggcgaaa
    22921 atcggtgtcg catctatgct cggtagtcgg gtacgggtga aaacctggag ctggtttgcc
    22981 gatgataaac aggaaatccg tcagggtggt tttgctggct ggttaaccga tggtacaccg
    23041 ctgtgggtga ctggcagtgg caccagcaaa acggtactga cccgttatgc gacggtgctt
    23101 aatcgggttt tgccagtgcc gacgcaagtt gccagtgggc aatgtgtaga ggtggagcta
    23161 tttgcgcgct agccgctgaa gaaaatcaca gcggagaaga gtacgacggc agtaaaaccc
    23221 ggcgtactta atgggcgcta tcgtgtgacg tttgccaatg gtaaccacat tacgtttgtc
    23281 agccacggtg agacgacctt actgaccgaa aagggcaaac tgaaattgca gtcgcatctc
    23341 gatcgtgaag agtacgttgc cagagttctg gatcgcgagg cgaaaagtac gccacctgag
    23401 gcagcgaaag ccatgactgt ggcgatacgc acttttttac agcaaaacgc caatcgggaa
    23461 ggcgattgcc tgaccatccc cgacagcagc gccacgcagc gtgtttctgc ttcgccagct
    23521 accacggggg cgcgaacgat gaccgtctgg acgcaggatc tcatctatgc gggcgatccg
    23581 gttcattatc acggcagtcg cgctaccgaa gggacgcttt cctggcggca agccatggcc
    23641 caggcgggga agggagagcg ttacgaccag atactcgcgt ttgcttatcc cgacaacagc
    23701 cttagccgct ggggagcgcc acgcacaacc tgtcagttat tacccaaagc gaaagcctgg
    23761 ctggcgaaaa aaatgccgca gtggcggcgt atattacaag gcgagacggg gtacaacgaa
    23821 ccagacgtgt ttgcggtctg tcgtttagtc tcaggtttcc cctataccga tcggcagcag
    23881 aaacggctgt ttattcgtaa cttctttacg cttcaggatc ggctggattt aacccatgag
    23941 tatctgcacc tggcctttga tggttatccc accgggcttg atgagaacta tatcgaaacg
    24001 ctgacccgac aattattaat ggactaatgc tatgcgaaag atttttcttc cgttgttact
    24061 ggtggcgctt tcgcctgtcg ctcacagtga aggtgtgcag gaagtcgaga ttgacgcacc
    24121 gctctctggc tggcatccgg cagagggcga agatgccagt ttttcacaat cgatcaatta
    24181 cccggcgtcg tccgtcaaca tggcagatga tcaaaatatc tcagcgcaga tccgcggcaa
    24241 aataaagaat tatgctgcgg cgggtaaagt tcagcagggc cggctggtgg tcaacggtgc
    24301 cagtatgccg cagagaattg aatccgatgg ttcatttgca cgcccttata ttttcactga
    24361 aggcagcaac agcgtgcagg tcatcagccc ggatgggcaa agccgacaaa aaatgcagtt
    24421 ttactcaacg ccgggtacag gaacgattcg tgcacgttta cggctggttc tctcgtggga
    24481 tacggacaat accgacctcg atcttcacgt tgtcacgccc gatggcgaac acgcctggta
    24541 cggtaacacc gtgctgaaaa acagtggtgc attggatatg gatgtcacga cggggtacgg
    24601 acccgagatt ttcgccatgc cagcaccagt tcacgggcgc tatcaggtgt atatcaacta
    24661 ctatggtggg cgcagcgaaa cggaattaac caccgcccaa ctgacgctga tcaccgatga
    24721 aggatcggtc aatgagaaac aggaaacatt tattgtaccg atgcgtaatg ctggcgagct
    24781 gacgctggtg aaaagttttg actggtgatc catcccttca cacaacctgg ctctttcttt
    24841 tttcagataa gactgctggg cgatctgccg acgccaggat ataaccatta acaagcgtcg
    24901 gcagattggt catttcttat ttttcgtact gagaactgtc catttactcc ggctttcctg
    24961 gttaaggcaa tggtttctaa gcgatacgca ttattgcaat ggtgacagaa cattgcggta
    25021 acacgctttt accgctacct taaccacact ccatcggtca cctgaggcgg agcttcgccc
    25081 ctttgaaata ccttgcttct tttcgtacaa ccctgaaagc ctcgcgctac atgttcagag
    25141 cattggcgtt agtgctctgg ctgttgattg ctttttcatc cgttttttac atcgttaatg
    25201 cgttacatca gcgagaatcg gaaattcgtc aggaatttaa tctgagttcc gatcaggctc
    25261 agcgctttat tcaacgcacc tctgatgtga tgaaagagct gaagtacatc gccgaaaatc
    25321 gcttatcggc agaaaacggt gtgctttccc cgcgtggacg agaaacgcag gcggatgtgc
    25381 ctgcgtttga accgctgttt gccgactccg attgttcagc aatgagtaac acctggcgag
    25441 gttctctgga gtcattggcg tggtttatgc gctactggcg cgataatttt tctgcggctt
    25501 acgatctcaa ccgggtattt ttaatcggca gcgataacct ctgcatggcc aatttcggtc
    25561 tgcgtgatat gccagtggaa cgcgataccg cgttgaaagc tttgcatgaa cgcatcaata
    25621 aatatcgaaa tgcaccacaa gatgatagcg gcagtaacct ctactggatc agcgaaggtc
    25681 cgcgccctgg cgtcgggtat ttttacgcgt tgacgccagt ttatctggcg aaccggttgc
    25741 aggcgctttt gggtgtcgag cagaccatcc ggatggagaa ctttttctta ccgggtacgt
    25801 tgccgatggg ggttaccatt cttgatgaaa atggtcatac cctgatttcg cttaccggac
    25861 cagaaagcaa aattaaggcc gatcctcgct ggatgcagga acgctcctgg tttggctata
    25921 cggaagggtt ccgggagctg gtgctgaaga aaaatctgcc accctcatcg ctcagcatcg
    25981 tgtattcggt gccggttgat aaggtgctgg aacgcattcg catgttgatc cttaacgcaa
    26041 ttttgctgaa tgtgcttgcc ggagctgcat tgtttactct cgcgcggatg tacgagcgac
    26101 gtattttcat tccggcggaa agcgacgccc tgcgactgga agaacatgag cagttcaatc
    26161 gcaagattgt cgcctccgcg ccagtgggta tctgcatttt gcgtaccgct gatggcgtca
    26221 atattttaag taacgaactg gcgcatacct atctcaatat gcttacgcat gaggaccgcc
    26281 aacgactgac acaaattatc tgtgggcagc aggtcaattt tgttgatgtc ctgaccagca
    26341 acaataccaa tctgcaaatc agcttcgtcc attcgcgcta tcgtaatgaa aacgtggcca
    26401 tttgtgtgct ggtggatgtt tcttcgcgcg tgaagatgga agagtcgttg caggagatgg
    26461 cacaagcagc ggaacaggcg agccagtcaa aatcgatgtt ccttgccacc gtcagtcatg
    26521 agctgcgaac gccgctgtat ggcattatcg gtaacctgga tctgttgcaa accaaagagt
    26581 taccgaaagg cgtcgatcgt ctggtgacgg caatgaacaa ctcttccagc ctgttgttga
    26641 aaattatcag cgatattctc gatttctcga agattgaatc ggaacagttg atgatcgaac
    26701 cgcgtgagtt ttcaccgcgt gaagtgatga accacatcac cgccaactat ttaccgctgg
    26761 tggtacgcaa gcagttaggc ttgtactgct ttattgaacc ggatgtgcca gtggccttaa
    26821 atggcgaccc gatgcgttta cagcaggtca tctccaacct gttgagtaac gccataaaat
    26881 tcaccgatac cggctgtata gttttgcatg tccgcgctga tggcgattat ctctctatcc
    26941 gtgttcgcga taccggcgtg gggattccgg cgaaagaagt ggtgcgcttg tttgatccct
    27001 tcttccaggt cggaacgggc gtacagcgaa acttccaggg gaccggtctg ggtctggcga
    27061 tttgtgaaaa actgatcagc atgatggacg gcgatatctc ggtagattca gaaccgggaa
    27121 tgggcagcca gtttaccgtg cgtattccgt tgtacggcgc tcagtacccg cagaaaaaag
    27181 gcgtggaagg gttgagtggt aaacgctgct ggctggcggt ccgcaatgcg tcgctctgtc
    27241 agttcctgga aaccagtttg cagcgcagcg gcatcgtcgt aacaacatac gaagggcagg
    27301 aaccgactcc cgaagatgtg ttaatcactg acgaggtagt gagtaaaaaa tggcagggca
    27361 gagcggtagt gaccttctgt cgtcgacata ttggtattcc gctggagaaa gcgccagggg
    27421 agtgggtaca cagtgtggct gctccgcatg agctaccggc attgttggcg cgtatttatt
    27481 tgatcgagat ggagagcgac gatcctgcta acgctctgcc gtcgacggac aaagcggtca
    27541 gcgataatga cgatatgatg attctggtcg tggatgatca tccgattaac cggcgtttgc
    27601 tggcagatca gttgggatcg ttgggctatc aatgtaaaac cgcgaatgat ggcgtcgatg
    27661 cgcttaatgt acttagcaag aatcatattg atatcgtgct tagcgacgtc aacatgccaa
    27721 atatggatgg ttaccgcttg acacaacgca ttcgtcagtt gggactgacg ttgccggtaa
    27781 tcggagtaac ggctaatgcg ttggctgaag agaagcagcg gtgtctggag tccggtatgg
    27841 acagctgcct gtcgaagcca gtaacgctgg atgttataaa acagacgctg acggtatatg
    27901 ccgagagggt caggaaatcg cgggaatcgt aggacggata aggcgtttac gccgcatctg
    27961 gcattcagtg caaatgccag atgcgacgct gacgcgtctt atctggccta ctttaatgta
    28021 atctgttgaa ataatgggaa tcgtaggacg gataaggcgt ttacgccgca tccggcaatc
    28081 ggtgcaaatg ccagataaga cactaacgcg tcttatctgg cctacagatg attagtcttt
    28141 atctgccgga cttaacgtta ctgaagagag ataattcagc agggcgatat cgttctcgac
    28201 acccagcttc atcatcgcag atttcttctg gctactgatg gttttaatac tgcggttcag
    28261 ctttttagcg atctcggtca ccaggaagcc ttccgcaaac aggcgcagaa cttcactctc
    28321 ttttggcgag agacgtttgt caccgtaacc tccagcactg attttttcca acaggcgaga
    28381 aacgctttcc ggggtaaatt tctttccttt ttgcagcgct gcgagggctt tcggcagatc
    28441 ggtcggtgca ccttgtttca gcacgatccc ttcgatatcc agatccaata ccgcactaag
    28501 aatcgccggg ttgttgttca tggtcagaac aataatcgac aggcttggga aatggcgctt
    28561 gatgtactta attaaggtaa tgccatcgcc gtacttatcg ccaggcatgg agagatcggt
    28621 aatcaacaca tgcgcatcca gtttcggcag gttgttgatc agtgctgtag agtcttcaaa
    28681 ttcgccgaca acattcaccc actcaatttg ctcaagtgat ttgcgaatac cgaacaagac
    28741 tatcggatgg tcatcggcaa taattacgtt catattgttc atgtaatagg ctaccttgct
    28801 acagcaagct cttgacataa ctgtcaatgt cgctgatgta tttttctatt cctggaacat
    28861 ccttctcacg aatcagatgt tccagcgttt cacataactg cttgccgggt accagattta
    28921 gcatggcaaa tacgccttta agacgatgag ccgtttgggc taacgcagca aagtcactgg
    28981 ttgctgcttc agtatacagc ctcttaacat catccggtac tgtgtctaca aagagcgcat
    29041 aatagccgct ggcatggagt tgcgcatttt catctccgcc cagaggcgat tctgtcacct
    29101 cttcctgcgc cagttgcact tcaattaatt gtaagaccgc ttcctgcata gcgttgctca
    29161 tattgaagtt gacgcacaat tgaccaggcc caatttcccg tacgccagac tcatcatcgc
    29221 ttaaaagcaa gccagaggca gtaagattag acggattatc cgttaaaaag atatcataat
    29281 cttgactaat taatctttca tcgggtgtga tacaggttgc accccaattt tctaactggc
    29341 gagtgacaat attccgaatt tctgccgaag taacatccac cattacgcag acatcatcca
    29401 gtaaacgctc ttcttcctct tcaacttccg ggtcagctgc gagcattttg atatgcacag
    29461 agtagcgtgt accaagccca tcccgcgttt tgatgtttaa atgaccgccc agtttacgtg
    29521 ccagttgatc gctcagccag aatgccagcg ggtccgcctt gccatagcga tcgttttggg
    29581 tctggttgat aaacgggaag tgcaaattat ccatttcatg aatgcttacg ccttctccgg
    29641 tgtccagaat gcggaacgtc aggcggtctt cggaggactc atcctgatca acctcaaggg
    29701 tgatttttcc caattgcgtt gaggtcacgg catattgcat cagtagcagc aaaatacgtc
    29761 gtaatgcatc gcgatcgccg cggcgcatat cgtgtgcttt cagatgattg ttaatcagca
    29821 gttgcagacc tttacgcttg atggcaggca acactgaagg cacaacttca tcaattaaat
    29881 cctgcacgga gaacagcacc gtctcacttt tccagctatc gtccgcaagc atgttcgcta
    29941 actgtatttc atcgaccaat cgcaccagca catctgcctg attcgccagt tgtttgcttt
    30001 ccggggcgtt gagtttagcc gcgctctccg ccagggactg tgcgggttct ttcagcgcat
    30061 cgccaatgtt tttcataaag gtcatccgcc cctgctggtt tttctcatac agacgctgcg
    30121 cctgcttgag tttcttgttt accagcactt cgcgatcctg atcgcgaata atgaaaattt
    30181 gtgtgcgcgg cgcaacctgg ctgcggaaca tgcggatctc atacagctcg ttattgatcg
    30241 tcgcctgaat aatcccctga tgctgttccg ccatggtggt gatgttttgc agattcaaat
    30301 gcggcagcaa atgatcggca attttgttac ttatgacagt gcggttcgat tcctgatcgt
    30361 gaaccagcag gccgagcggc agcagtgaga ctatctcttc attgattgcc cgtaaaatgc
    30421 gcaattcgtt attgaccgcc gtgttgggta gactttctgt actgcgactg gagaaatggc
    30481 ggaatgtggt atagccaaat aacgccagcg ccagcaaacc gatgttcagc agcagtggca
    30541 gcagaatgtt ttgcaacgta tccagcaata aggtgccata aggaacctgc cagaccaggc
    30601 gcatatcggt agagttgagt gccgaggaga tttcaatctt cgtactgtta aagtggatac
    30661 tgacgctatc cgttccttct ttctcattat cattgtttcc cgttgccgtc gcgtctggct
    30721 caaggcggaa actgtccagc ggcatacccg gtgggatcaa atcattaatc ggcagatcaa
    30781 aagccacgac cgttgccaga tgtcccggct ggttgaacgt ggtacgcaag gtaaagtaat
    30841 gaccgttctg ccaggccagg cggcgcatgt tagaaaaact ttcgcgttca tcgagggcgt
    30901 tggcctgttg caacatctct gcacgacgtg aatcaacaat gtcactgacg gtcgattctt
    30961 taaatccgga ggtgagatct ttgaggggca gggttgagat cagcaccaga ctgttatcct
    31021 gaccattcag gtaatacatt gaccacggta cattttctgc gccccacaat gtgtccagat
    31081 aggtggacat ccgttgagtc atctcaagcg ttgagttgtc gtgagagcca aagattaacg
    31141 cttccgtttt gcggcgcggt ttttccagat agtagacatc ctgtttcagg cgcgtctctt
    31201 gtaaaccttc gccggaggag ggggaggtcg tcgcggcaat gttgtcgtag atctgccagg
    31261 tcacgtaacg ccaggtatcg acgcgctttt ggatagcgtg ggtaatgtcg acaatttggt
    31321 aacttttatc tttcagccag gcgttaacgg cgctttgtac cattacaccc atcgtcacca
    31381 gtaacacaat gatcaacagt aaaaagaagc gggtaatgct ccccggtagg agtgaaaagc
    31441 gggtcgtggc cgttgtctct ttctgacgca ttcgtgttta tgacctgtta aaacttcgcg
    31501 aagtgatagg gcagtataaa gggtacagtg tgaaattcca gactcttacc tgattgatga
    31561 ttctctattc tcccgtgata tttatcatcc tgcattgggg gaaatagatg tacaagcgcc
    31621 attttgctgt acaaataacc tacaaaaagc ccaacgaaaa taattgcaaa taaacaaaga
    31681 ttgctggaaa ttatgctgat gttaattatt tgtgaaatag ttaacaagcg ttatagtttt
    31741 tctgtggtag cacagaataa tgaaaagtgt gtaaagaagg gtaaaaaaaa ccggatgcga
    31801 ggcatccggt tgaaataggg gtaaacagac attcagaaat gaatgacggt aataaataaa
    31861 gttaatgatg atagcggtag ttattctagt tgcgagtgaa ggttttgttt tgacattcag
    31921 tgctgtcaaa tacttaagaa taagttattg attttaacct tgaattatta ttgcttgatg
    31981 ttaggtgctt atttcgccat tccgcaataa tcttaaaaag ttccctcgaa tttacatttt
    32041 gaaacatcta tagcgataaa tgaaacatct taaaagtttt agtatcatat tcgtgttgga
    32101 ttattctgca tttttgggga gaatggactt gccgactgat taatgagggt taatcagtat
    32161 gcagtggcat aaaaaagcaa ataaaggcat ataacagagg gttaataaca tgaaagttaa
    32221 agtactgtcc ctcctggtcc cagctctgct ggtagcaggc gcagcaaacg ctgctgaagt
    32281 ttacaacaaa gacggcaaca aattagatct gtacggtaaa gtagacggcc tgcactattt
    32341 ctctgacgac aagtctgtag atggcgacca gacctacatg cgtcttggct tcaaaggtga
    32401 aactcaggtt actgaccagc tgaccggtta cggccagtgg gaatatcaga tccagggcaa
    32461 cagcgctgaa aacgaaaaca actcctggac ccgtgtggca ttcgcaggtc tgaaattcca
    32521 ggatgtaggt tctttcgact acggtcgtaa ctacggcgta gtttacgacg taacttcctg
    32581 gaccgacgtt ctgccagaat tcggtggtga cacctacggt tctgacaact tcatgcagca
    32641 gcgtggtaac ggtttcgcga cctaccgtaa cactgacttc ttcggtctgg ttgacggcct
    32701 gaactttgct gttcagtacc agggcaaaaa cggtagcgta agcggcgaag gcatgactaa
    32761 caacggtcgt gaagcactgc gtcagaacgg cgacggcgtt ggcggatcta tcacttatga
    32821 ttacgaaggc ttcggtatcg gtgctgcagt ttccagctcc aaacgtactg atgatcagaa
    32881 cagcccgctg tacatcggta acggcgaccg tgctgaaacc tacactggtg gtctgaaata
    32941 cgacgctaac aacatctatc tggctgctca gtacacccag acctacaacg caactcgcgt
    33001 aggttccctg ggttgggcga acaaagcaca gaacttcgaa gctgttgctc agtaccagtt
    33061 cgacttcggt ctgcgtccgt ccctggctta cctgcagtct aaaggtaaaa acctgggtgt
    33121 catcaatggt cgtaactacg acgacgaaga tatcctgaaa tatgttgatg ttggcgcgac
    33181 ctactacttc aacaaaaaca tgtccaccta tgttgactac aaaatcaacc tgctggacga
    33241 caaccagttc actcgtgacg ctggcatcaa cactgataac atcgtagctc tgggtctggt
    33301 ttaccagttc taatctcgat tgatatcgaa caaagggcct gcgggccctt ttttcattgt
    33361 tttcagcgta caaactcagt tttttggtgt actcttgcga ccgttcgcat gaggataatc
    33421 acgtatggaa ataagcttta cccgcgtggc actgctggct gccgcgctct tctttgttgg
    33481 ttgcgatcaa aaaccacaac ccgccaaaac ccacgctact gaagttaccg ttcttgaagg
    33541 caaaactatg ggtaccttct ggcgtgccag catcccgggc attgacgcca aacgcagcgc
    33601 cgaacttaaa gaaaagattc agacccagct ggacgccgac gatcagctgc tttcgaccta
    33661 taaaaaagat tccgcgctga tgcgctttaa cgactcgcaa agtttatcgc cgtggccggt
    33721 aagtgaagcg atggccgata tcgtcaccac ctcgctacgc attggcgcga agaccgatgg
    33781 cgcgatggat ataaccgtcg ggccgctggt gaatctgtgg ggtttcggtc cggaacaaca
    33841 gccggttcaa attccgagcc aggaacagat cgacgcgatg aaagccaaaa ctggtttaca
    33901 gcaccttacg gtcattaatc agtcgcatca gcaatatctg caaaaagacc tgccggattt
    33961 atatgtcgat ctctccaccg tcggcgaagg ttatgcggcg gatcacctgg cacgcttgat
    34021 ggagcaggaa gggatttccc gctatctggt gtcggtgggc ggcgcgctga acagccgtgg
    34081 tatgaacggt gaaggcctgc cgtggcgggt agcgattcaa aaaccaaccg ataaagaaaa
    34141 cgcggttcag gccgtggtgg atatcaacgg tcatgggatt agcacctctg gcagctaccg
    34201 taactattac gaactggacg gcaaacgtct ttcccatgtt atcgatccgc aaaccgggcg
    34261 tcccatcgaa cacaatctgg tatccgtgac ggtgattgca ccgacggcgc tggaagccga
    34321 cgcctgggat acaggtttga tggtactcgg gccggagaaa gccaaagaag ttgttcgccg
    34381 ggaagggctg gcggtttata tgatcaccaa agaaggcgat agctttaaaa cctggatgtc
    34441 accacagttt aaaagcttcc ttgtcagcga aaaaaattaa agcgcaagat tgttggtttt
    34501 tgcgtgatgg tggccgggca gactaaaggc tatccttaac cagggagctg attatgaaaa
    34561 acgccacatg cttaactgac gatcaacgct ggcaatctgt cttagcccgc gacccgaatg
    34621 ccgacggcga attcgttttc gccgtgcgca ccacaggcat cttttgccgt ccgtcttgcc
    34681 gcgccagaca tgctttgcgg gaaaacgtct ccttctacgc aaatgccagc gaggcactcg
    34741 ccgccggatt tcgcccctgc aaacgctgcc aacctgataa agccaatgcc cagcaacatc
    34801 ggttggataa aatcacccac gcgtgtcgac tgctggaaca ggaaacgcct gtaacgttgg
    34861 aagccttagc cgaccaggtg gcgatgagtc catttcatct acatcggttg tttaaagcga
    34921 ctaccggaat gacgcctaaa gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat
    34981 cgctggcgaa aggggagagc gtgacgacgt ctattcttaa cgccggattc cccgacagca
    35041 gcagttacta ccacaaagcc gatgaaacgc tgggcatgac ggctaaacaa ttccgtcacg
    35101 gtggcgaaaa tctggcggtg cgttacgcgc tggctgattg tgagctgggt cgttgcctgg
    35161 tggcagaaag cgagcggggg atttgcgcga tattgctggg cgatgatgac gcgacattaa
    35221 tcagcgagtt gcagcagatg tttcccgctg ccgacaacgc gcctgccgat ctgacgtttc
    35281 agcaacatgt gcgtgaagtg atcgccagcc tcaatcaacg cgatactccg ctgacgttac
    35341 cgctggatat tcgcggcact gcttttcagc aacaagtctg gcaggcactg cgcacgatac
    35401 cttgcggtga aaccgtcagt tatcagcaac tggctaacgc catcggcaaa ccgaaagcgg
    35461 tacgggccgt tgccagcgcc tgtgccgcca acaagctggc tatcgtaatt ccctgtcatc
    35521 gagtggtccg tggtgatggc acactttccg gttaccgctg gggcgtgtcg cgtaaagcgc
    35581 aactgctgcg ccgcgaagct gaaaatgagg agaggtaatg ttggatctgt ttgccgatgc
    35641 tgaaccgtgg caagagccac tggcggctgg tgcggtaatt ttacggcgtt ttgcttttaa
    35701 cgctgcagag caactaatcc gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca
    35761 gatggtcacc cccgggggat ataccatgtc ggtggcgatg accaactgtg ggcatctggg
    35821 ctggacgacc catcggcaag gttatctcta ttcgcccatt gatccgcaaa caaataaacc
    35881 gtggcccgcc atgccgcaga gttttcataa tttatgtcaa cgtgcggcta cggcggcggg
    35941 ctatccagat ttccagccag atgcttgtct tatcaaccgc tacgctcctg gcgcgaaact
    36001 gtcgctgcat caggataaag acgaaccgga tctgcgcgcg ccaattgttt ctgtttctct
    36061 gggcttaccc gcgatttttc aatttggtgg cctgaaacgt aatgatccgc tcaaacgttt
    36121 gttgttggaa catggcgatg tggtggtatg gggcggtgaa tcgcggctgt tttatcacgg
    36181 tattcaaccg ttgaaagcgg ggtttcatcc actcaccacc gactgccgct acaacctgac
    36241 attccgtcag gcaggtaaaa aagaataaaa ataagaatta ttattgctgt gcgcgcgaag
    36301 atgtttaaac tgcgggctgt aattcattgt ccgggttttc tgcatggaac ttcttgtact
    36361 tgtctggcgg cagtatcgct ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc
    36421 ggctttaggc attggcttaa ttgcttttat caatcagcgc cttatcgaaa cggcggatac
    36481 cagtctgctg gtgttgccgg agtttctggg attattgctg ctgttgatgg cagtcactct
    36541 cggatcgcaa ctggcgctca ccactttggg gcatcacttc gtttaccgac tgcgtagtga
    36601 atttatcaag cggattctgg atactcacgt cgagcgcatt gaacaactcg gtagcgcctc
    36661 gttgctggcg ggattaacca gcgatgtgcg caatatcacc attgcttttg tgcgtctgcc
    36721 ggaactggtg caggggatca ttctcactat cggttcagcg gcgtatctgt ggatgctgtc
    36781 gggcaaaatg ttgctggtaa cggctatctg gatggcagtc accatctggg gcggttttgt
    36841 gctggtggcg cgggtgtaca aacatatggc gaccctgcgt gaaaccgaag acaagctgta
    36901 cacggatttt caaactgtac ttgaagggcg caaagagctg actctgaacc gggaacgcgc
    36961 cgagtatgtg tttaacaacc tctacattcc tgatgcgcaa gagtatcgcc atcatattat
    37021 tcgcgcagac accttccatc ttagtgccgt gaactggtca aacatcatga tgctgggcgc
    37081 tatcggcctg gtgttctgga tggcgaacag tctcggttgg gctgatacca acgttgccgc
    37141 gacctattcg ttgacgcttt tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt
    37201 gccgacgctg ctgacggcgc aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc
    37261 tttcaaagca gagtttccgc gcccgcaggc gtttcccagc tggcaaacgc tggagctgcg
    37321 taacgtgacg tttgcttatc aggataacgc gttttccgtt ggtccgatta atctcaccat
    37381 caaacgtggc gagctgctgt ttctgattgg cggcaacggt agcggaaaat cgacgctggc
    37441 gatgttgttg acgggcttgt atcagccaca aagcggcgaa atcttgctgg atggcaaacc
    37501 tgtcagcggc gaacaaccgg aagattatcg caaactgttt tcggcagtgt ttaccgatgt
    37561 ctggctgttt gatcaactgc tggggccgga gggtaaaccc gctaacccgc aactggttga
    37621 gaagtggctg gcgcagctga aaatggctca taagcttgag ttaagaaacg ggcgtattgt
    37681 taacctgaag ttatcaaaag ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga
    37741 agaacgcgat attatcctgc tggatgaatg ggcggcggat caggatccac acttccgtcg
    37801 tgagttttat caggtgttgc tgccgctgat gcaggagatg ggtaaaacta ttttcgccat
    37861 cagtcatgat gatcattact ttatccacgc cgaccgcctg ctggaaatgc gcaacgggca
    37921 acttagcgag ctgacgggcg aagagcgcga tgccgcttcg cgtgatgccg ttgcccggac
    37981 ggcataatat tacgacgctg acctggcgga ctgctgccgc caggtcaata tgctaaaaat
    38041 cccttcattt ctaataaccc tataattaat caacgaaatt ataatgtttc taa
//
LOCUS       DAZFBR010000035        37924 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFBR010000035 DAZFBR010000000
VERSION     DAZFBR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.035"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(137..832)
                     /gene="aqpZ"
                     /locus_tag="ACHJZ2_001997"
     CDS             complement(137..832)
                     /gene="aqpZ"
                     /locus_tag="ACHJZ2_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO4568116.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1258..2916
                     /gene="ybjD"
                     /locus_tag="ACHJZ2_001998"
     CDS             1258..2916
                     /gene="ybjD"
                     /locus_tag="ACHJZ2_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO4568117.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2913..3869)
                     /gene="ybjX"
                     /locus_tag="ACHJZ2_001999"
     CDS             complement(2913..3869)
                     /gene="ybjX"
                     /locus_tag="ACHJZ2_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO4568118.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4020..5135
                     /gene="macA"
                     /locus_tag="ACHJZ2_002000"
     CDS             4020..5135
                     /gene="macA"
                     /locus_tag="ACHJZ2_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO4568119.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5132..7078
                     /gene="macB"
                     /locus_tag="ACHJZ2_002001"
     CDS             5132..7078
                     /gene="macB"
                     /locus_tag="ACHJZ2_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO4568120.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7151..7375)
                     /gene="cspD"
                     /locus_tag="ACHJZ2_002002"
     CDS             complement(7151..7375)
                     /gene="cspD"
                     /locus_tag="ACHJZ2_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO4568121.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7525..7599
                     /gene="yljB"
                     /locus_tag="ACHJZ2_002003"
     CDS             7525..7599
                     /gene="yljB"
                     /locus_tag="ACHJZ2_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO4568122.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7698..8018
                     /gene="clpS"
                     /locus_tag="ACHJZ2_002004"
     CDS             7698..8018
                     /gene="clpS"
                     /locus_tag="ACHJZ2_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO4568123.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8049..10325
                     /gene="clpA"
                     /locus_tag="ACHJZ2_002005"
     CDS             8049..10325
                     /gene="clpA"
                     /locus_tag="ACHJZ2_002005"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO4568124.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10671..10758)
                     /locus_tag="ACHJZ2_002006"
     tRNA            complement(10671..10758)
                     /locus_tag="ACHJZ2_002006"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10722..10724),aa:Ser,seq:gga)
     gene            complement(11012..11230)
                     /gene="infA"
                     /locus_tag="ACHJZ2_002007"
     CDS             complement(11012..11230)
                     /gene="infA"
                     /locus_tag="ACHJZ2_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO4568125.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11515..12219)
                     /gene="aat"
                     /locus_tag="ACHJZ2_002008"
     CDS             complement(11515..12219)
                     /gene="aat"
                     /locus_tag="ACHJZ2_002008"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO4568126.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12261..13982)
                     /gene="cydC"
                     /locus_tag="ACHJZ2_002009"
     CDS             complement(12261..13982)
                     /gene="cydC"
                     /locus_tag="ACHJZ2_002009"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO4568127.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13983..15749)
                     /gene="cydD"
                     /locus_tag="ACHJZ2_002010"
     CDS             complement(13983..15749)
                     /gene="cydD"
                     /locus_tag="ACHJZ2_002010"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO4568128.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15872..16837)
                     /gene="trxB"
                     /locus_tag="ACHJZ2_002011"
     CDS             complement(15872..16837)
                     /gene="trxB"
                     /locus_tag="ACHJZ2_002011"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO4568129.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17382..17876
                     /gene="lrp"
                     /locus_tag="ACHJZ2_002012"
     CDS             17382..17876
                     /gene="lrp"
                     /locus_tag="ACHJZ2_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO4568130.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18011..22039
                     /gene="ftsK"
                     /locus_tag="ACHJZ2_002013"
     CDS             18011..22039
                     /gene="ftsK"
                     /locus_tag="ACHJZ2_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO4568131.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLSPPPFD"
     gene            22194..22805
                     /gene="lolA"
                     /locus_tag="ACHJZ2_002014"
     CDS             22194..22805
                     /gene="lolA"
                     /locus_tag="ACHJZ2_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO4568132.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22816..24159
                     /gene="rarA"
                     /locus_tag="ACHJZ2_002015"
                     /gene_synonym="mgsA"
     CDS             22816..24159
                     /gene="rarA"
                     /locus_tag="ACHJZ2_002015"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO4568133.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24250..25542
                     /gene="serS"
                     /locus_tag="ACHJZ2_002016"
     CDS             24250..25542
                     /gene="serS"
                     /locus_tag="ACHJZ2_002016"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO4568134.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25781..28225
                     /gene="dmsA"
                     /locus_tag="ACHJZ2_002017"
     CDS             25781..28225
                     /gene="dmsA"
                     /locus_tag="ACHJZ2_002017"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO4568135.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28236..28853
                     /gene="dmsB"
                     /locus_tag="ACHJZ2_002018"
     CDS             28236..28853
                     /gene="dmsB"
                     /locus_tag="ACHJZ2_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO4568136.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28855..29718
                     /gene="dmsC"
                     /locus_tag="ACHJZ2_002019"
     CDS             28855..29718
                     /gene="dmsC"
                     /locus_tag="ACHJZ2_002019"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO4568137.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29754..30380)
                     /gene="ycaC"
                     /locus_tag="ACHJZ2_002020"
     CDS             complement(29754..30380)
                     /gene="ycaC"
                     /locus_tag="ACHJZ2_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO4568138.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30695..31843
                     /gene="ycaD"
                     /locus_tag="ACHJZ2_002021"
     CDS             30695..31843
                     /gene="ycaD"
                     /locus_tag="ACHJZ2_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4568139.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32053..33483
                     /gene="ycaM"
                     /locus_tag="ACHJZ2_002022"
     CDS             32053..33483
                     /gene="ycaM"
                     /locus_tag="ACHJZ2_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO4568140.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33484..33594)
                     /locus_tag="ACHJZ2_002023"
                     /pseudo
     CDS             complement(33484..33594)
                     /locus_tag="ACHJZ2_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(33693..34433)
                     /gene="pflA"
                     /locus_tag="ACHJZ2_002024"
     CDS             complement(33693..34433)
                     /gene="pflA"
                     /locus_tag="ACHJZ2_002024"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO4568141.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34625..36907)
                     /gene="pflB"
                     /locus_tag="ACHJZ2_002025"
     CDS             complement(34625..36907)
                     /gene="pflB"
                     /locus_tag="ACHJZ2_002025"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO4568142.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(36962..37819)
                     /gene="focA"
                     /locus_tag="ACHJZ2_002026"
     CDS             complement(36962..37819)
                     /gene="focA"
                     /locus_tag="ACHJZ2_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO4568143.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9372 a         9306 c        10063 g         9183 t
ORIGIN      
        1 acgcgccctc agcccacatc catgtgggct gccccggcct tcggggaacg cttcagcgat
       61 tttgacgcca ccaatacccg tgcggtttgt aatgtcgaag ataaaacaac cagggcagat
      121 aaaatgccaa tgctaattaa ttacgctttt ccagcagagt ccggtaaatc agaccaccga
      181 taatgccacc gacaattggc accacccaga agaaccacag ttgttctaat gcccagccgc
      241 cctggaagat ggcaaccgcg gtgctgcgcg ccgggttaac agaagtgtta gtcaccggaa
      301 tactgattaa gtgaatcagg gttaaggcca gaccaatagc gatcggcgca aaacctgccg
      361 gagcgaattt gtcggttgca ccgtggatca ctaacaggaa gcccgcactc aataccagtt
      421 caactaccag cgcggaaagc atggaataac cgcctggtga atgctcgcca taaccgttag
      481 aagcaaaacc gctggctgcc gcgtcaaaac ccgttttacc actggcaatt aaatacagca
      541 gcgctgccgc aacaataccg ccgacaacct gggcaattac gtagccaacg acttcttttg
      601 ccggaaaacg tccgccagcc cataaaccaa tagtgaccgc cgggttaaaa tgaccaccag
      661 aaatatgacc aacagcaaag gccatcgtca gaacggtcag accgaacgcc aacgccacgc
      721 cggcaaaacc aatgcctaat tccgggaagc ctgcggccag tacagcacta ccacagccac
      781 caaaaacaag ccagaaagta ccaaaacatt cagctgctaa ttttctgaac atatccacct
      841 caattaaaaa ttgaccctgt gaaaaatatg gtcgttttat agggtcgtcg taaaaaagtg
      901 acgacggaaa taatgcgcgg ctattttaaa aacgaaggcg agtcattcac cagataaata
      961 aatccagtaa atttgattta gggcaacagc gggttgcccc atatagtcat ttgtctgatt
     1021 gacagtgtag tgcaagcaaa agatttaatc ctttaggcgt aataaaaaat aatttatcat
     1081 gctaattatt tgattttgtt gtttttgcag acttatcagc aagagggagt ataacgcgat
     1141 tattcgctca tttttcagac atttgccatg cttaaatgtg atgtcatcac gtattagcaa
     1201 ggcctttccc gttatactgc cagcgtaaag gataagtcac atatttctgg aggggatatg
     1261 attcttgagc gcgttgaaat tgtgggtttt cgcggtatca accgtttgtc gttgatgctg
     1321 gaacaaaaca acgtcctgat tggggagaac gcgtggggta aatccagctt gctggacgcc
     1381 ttaaccctgc tgctatcgcc agaatcagat ctctaccatt ttgagcgcga cgatttctgg
     1441 ttcccgccgg gagatatcaa cgggcgagaa catcatctgc atattatttt gaccttccgc
     1501 gaatcgctgc caggtcgaca tcgggttcgc cgttatcggc cgctggaagc gtgctggacg
     1561 ccatgcaccg atggctatca ccgtattttt tatcgtctgg aaggggagag tgcggaagac
     1621 ggcagcgtga tgacactgcg cagttttctc gataaagacg gacatccgat tgatgttgag
     1681 gatattaacg atcaggcaca ccatctggtg cgtttaatgc cggtgctgcg cttgcgtgat
     1741 gcccgtttta tgcgccgtat tcgtaacggc acggtgccaa atgtccccaa tgtggaagtc
     1801 accgcgcgcc agctcgattt cctcgcccgt gagttgtcct cacatccgca aaatctctct
     1861 gatggacaga ttcgtcaggg actttccgca atggtgcagt tgcttgagca ttatttctct
     1921 gagcaggggg ctggacaggc gcgatatcgt ttaatgcggc ggcgggccag caatgagcaa
     1981 cgaagctggc gctatctgga catcatcaac cggatgattg accgacctgg agggcgctcg
     2041 tatcgggtta ttttgctcgg cctgtttgct actttattgc aggcaaaagg cacattgcga
     2101 ctggataaag acgcccgtcc attgttgctg atcgaagatc cagaaacgcg tttacacccc
     2161 attatgcttt cagttgcctg gcatctgttg aatcttttgc ctttacaacg tattgctacc
     2221 accaactctg gcgagctgct ttcgttaact ccagtggaac atgtttgccg cctggtgcgt
     2281 gaatcttctc gtgttgccgc ctggcgtctg gggccaagtg gcttgagcac cgaagacagc
     2341 cgccgcatct cttttcacat tcgttttaat cgtccgtcat cgctgtttgc tcgctgctgg
     2401 ttgctggtgg aaggggaaac ggaaacctgg gttatcaatg aactggcgcg tcagtgcgga
     2461 catcattttg atgccgaagg gatcaaggtc attgagtttg cccagtccgg gctaaagcca
     2521 ctggttaaat ttgcccgccg aatggggatt gaatggcatg tactggtcga tggcgatgaa
     2581 gcagggaaga aatatgccgc tacggtacgc agcctgttga ataatgatcg ggaagccgaa
     2641 cgagaacatt taacggcgtt accggcgctg gatatggaac attttatgta tcgccaggga
     2701 ttttccgatg tgttccaccg cgtggcgcaa atcccggaaa atgtcccgat gaatctgcgc
     2761 aaaattatct cgaaagcgat ccatcgctct tccaaacccg atcttgccat tgaagtggca
     2821 atggaggccg gacgtcgtgg tgtggactcc gtaccgacgc tgctgaaaaa aatgttctca
     2881 cgcgtgctgt ggctggcgcg cggtcgcgcg gattaaccgc gaaacatcgt ggccatttgt
     2941 ggctgaatag cgtcgagcat ctcatagcgc cgacggtatt cagcccgttt tttactggcg
     3001 atttcggcaa tctcttttcg tgctatctgt gctggaaggc ggtaatggcg ttcagcatca
     3061 catacgccgc caaccgattc ccagaaagcg ttgtaatcgg catggatctt gccttcttta
     3121 tcgcgataac gcaggctgcg gtaaatatgc gtttcattgc tgacggcaat aatctgctct
     3181 acctgcaaac gttgggcaaa cagacaggcc gcttccatca cgaggcgttt gggaaatagc
     3241 ccgtggcagg ctttcgtcgc attctggatt tcctgatgtg gaatttccca ttttgcgcct
     3301 tgcagtccac caataaacat cgttcttttc ccctgatatt cacacagggt aaacgtgatt
     3361 tctgccagag gaataccttc gctgttgcgg aacaggattg tgctgtcacc ttctttatcc
     3421 attgagatca tcatggtcag ctcaagcgtg aactgctcgc cgtttttgcc ttccagcttc
     3481 gccagttgca gcccgggggt attcaaatat aagctgaatt cttccgccga catacatcca
     3541 cggagtaacg cataatggta acgtaacgcc tccagcaatt gcttacggct aagattcgcc
     3601 gcaaggtaag ggcgatgcag acgcacaggc agtcgcggct ggcgcgttaa caatacattg
     3661 agattaggcc agtgggaaag ttcgttcatc cactcaacgc ttaaacgcgg cataatcaac
     3721 gagcgcagca aaaatttctg gcgaaaacta cggcgatgcc agaatttacc cggccgacac
     3781 tgtccacgtg ccagactaag aaaaagtgac aggctgctga gagattcaga tgacgtaaag
     3841 gtccgttcgg ttagctgcga catattcatg aaatcaatgg ttgtacatga cgtcgatttc
     3901 accattgcgt atcttaacca aacatcaata gtgtgattac taacgtaaat tttagggttt
     3961 tgttgatatt tcgttgaagt taatgacccg gattggcata tggagtattc agaatattta
     4021 tgaaaaagcg gaaaaccgtg aagaagcgtt acgttattgc gctggtgata gtcatcgccg
     4081 gactgattac gttatggaga attcttaacg cacccgtgcc gacttatcag acactgattg
     4141 tgcgccccgg tgatttacag caaagcgtgc tggcgaccgg aaagctggac gcgctgcgta
     4201 aggttgacgt gggcgcgcag gtcagcggtc agttgaaaac gctgtcggtg gcgattggcg
     4261 ataaagtaaa aaaagaccag cttttagggg ttattgatcc tgaacaggct gaaaaccaga
     4321 tcaaggaggt cgaagcaacg ctgatggagc tacgcgcgca gcggcagcag gcggaagcgg
     4381 agctgaaact ggcgcgggtg acgtattccc gtcagcaacg tctggcacaa acgcaggcgg
     4441 tttcactgca ggatctcgac accgccgcga cggagatggc tgtgaaacag gcgcaaattg
     4501 gcaccattga cgcgcaaatc aagcgcaatc aggcttctct cgatacggct aaaaccaatc
     4561 tcgattacac tcgcatcgtt gccccgatgg ccggggaagt cacgcaaatc accactctgc
     4621 aaggccagac ggtgattgcc gcacaacaag caccgaacat tctgacgctg gcagatatga
     4681 gcaccatgct ggtaaaagcg caggtttctg aagcggatgt aatccacctg aagccggggc
     4741 aaaaagcctg gtttacggta cttggcgatc cactgacgcg ctacgagggg caaatcaagg
     4801 atgtactacc gacgccggaa aaggttaacg acgctatttt ctattacgcc cgttttgaag
     4861 tccccaaccc caatggtttg ctgcggctgg atatgactgc gcaagtgcat attcagctca
     4921 ccgatgtgaa aaatgtgctg acgatccctc tgtcggcgtt aggcgatccg gttggcgata
     4981 atcgttataa agtcaaattg ttgcgtaatg gtgaaacacg cgagcgtgaa gtgacgattg
     5041 gcgcacgtaa cgataccgat gttgagattg tcaaagggct ggaagcgggc gatgaagtgg
     5101 tgattggtga ggccaaacca ggagctgcac aatgacgcct ttgctcgaat taaaggatat
     5161 tcgtcgcagc tatcctgccg gtgatgagca ggttgaggtg ctgaagggca tcaccctcga
     5221 tatttatgcc ggtgagatgg tagcgattgt tggcgcttcg ggttccggta aatcgaccct
     5281 gatgaatatt ctcggctgtc tggataaggc caccagcggc acctatcgcg tcgccggtca
     5341 ggatgttgcc acgctggacg ccgatgcgct ggcgcaactg cgccgcgagc atttcggctt
     5401 tattttccag cgttaccatt tgctttcgca tttaaccgcc gagcagaacg ttgaagtacc
     5461 agccgtctat gctggtcttg agcggaaaca gcggctgctt cgcgcccagg agttgctgca
     5521 acgactgggg ctggaagacc gtacagagta ttatccggca cagctttcgg gtggtcagca
     5581 acagcgcgtt agcatcgcgc gggcattgat gaacggcggt caggtaattc ttgccgatga
     5641 accgaccggc gcactggaca gccattcagg cgaagaggtg atggcgatcc tgcatcagct
     5701 gcgcgatcgc gggcatacgg tgattatcgt cactcacgat ccgcaggtcg ctgctcaggc
     5761 cgagcgggtg atcgaaattc gcgacggcga aattgtgcgc aatcctcccg ccattgaaaa
     5821 agtgaatgtt gctggcggga cggaacctgt tgtcaacacg gcgtctggct ggcggcagtt
     5881 tgtcagcggt tttaatgagg cgctgacgat ggcatggcgg gcgctggcag cgaataaaat
     5941 gcgtacttta ctgaccatgc tggggattat aatcggtatt gcgtcggtgg tttccattgt
     6001 cgtggtgggc gacgccgcca aacaaatggt gctggcggat attcgttcta ttggtacgaa
     6061 tactattgat gtctatcccg ggaaagattt tggcgatgac gatccgcaat atcaacaggc
     6121 gctgaagtac gatgacttaa tcgccatcca aaaacaaccg tgggtcgctt cagccacacc
     6181 tgccgtctcg caaaacctgc gcctgcgtta taacaatgtt gatgttgctg ccagtgccaa
     6241 tggcgtgagc ggcgattatt ttaatgtcta tggcatgacc ttcagtgaag gaaacacctt
     6301 taaccaggaa cagctgaacg gtcgtgcgca ggtcgtggtt ctcgacagta atactcgccg
     6361 ccagcttttc ccccataaag cagatgtggt tggcgaggtg attctggtcg gcaatatgcc
     6421 cgccagagtc attggtgtgg cggaagaaaa acagtcgatg tttggtagca gtaaagtgct
     6481 gcgtgtctgg ctaccttaca gcacgatgtc cgggcgagtt atgggccagt cgtggctgaa
     6541 ctccattact gtcagggtga aagaaggatt tgacagcgcc gaggcggaac agcaactcac
     6601 gcgtttactt tcactgcgcc acggtaagaa ggatttcttt acctggaaca tggacggcgt
     6661 cttgaaaacc gttgaaaaga ccacacgtac tttacaactg tttatgacgc tggtggcggt
     6721 gatttcgctg gtagtgggcg gtattggtgt aatgaatatt atgctggtgt cagtgaccga
     6781 gcggacgcgg gaaattggca ttcgcatggc tgtaggtgcg cgagcaagcg atgttttgca
     6841 acagttcctg atcgaagccg tactggtttg cctggtcggt ggcgcgttgg gaataacact
     6901 gtcactgtta attgctttca ccttgcagct tttcttaccc ggctgggaga ttggtttttc
     6961 accgttggca ctgctgctgg cgtttctctg ctcgacggtc accgggattt tatttggctg
     7021 gttacccgca cgaaatgcgg cacgactgga tccagtagat gctctggcac gagagtaatt
     7081 tttgagataa aaatgccagc cgatcgggct ggcattttgc ctttaggatg tacacaatga
     7141 gacagaagag ctatgcgact gccgcttcta cttcgacggg cacaataaca ctggcgtgat
     7201 tgccttttgg cccctggtgg acatcaaact gaacggattg tccagctttt agcgttctgt
     7261 aaccatccat ctgaatggtg gaataatgag cgaaaatatc ttcgccgccg ccttcagggc
     7321 agatgaaacc aaaccctttg gcattgttga accacttaac agtacccttt tccatgcttc
     7381 gacatccttc gcaaatctta tacaagtaag atggaataaa ccggggtcag agagggggct
     7441 gttcaaaacc tcgccaactc tagaaataca atttagagaa ttagggcgag ccgtcaagca
     7501 tttgacaggg gacagggggc aggtatgaat caaaaatttg aagcagttaa cgctattgac
     7561 aggaatgtga cagatgtcgc tgatgccaac gatagatgat agttatctat catgtggagt
     7621 agattggtca ggcaaataag ctcttgtcag cggcagggcg ttctgccgat aaccgtaacc
     7681 gaagatgata actgacaatg ggtaaaacga acgactggct ggactttgat caactggcgg
     7741 aagaaaaagt tcgcgacgcg ctaaaaccgc catctatgta taaagtgata ttagtcaatg
     7801 atgattacac tccgatggag tttgttattg acgtgttaca aaaattcttt tcttatgatg
     7861 tagaacgtgc aacgcaattg atgctcgctg ttcactacca ggggaaggcc atttgcggag
     7921 tctttaccgc cgaggttgca gaaaccaaag tggcgatggt gaacaagtac gcgagggaga
     7981 atgagcatcc attgctgtgt acgctagaaa aagcctgaat gcaggcataa aaattggggg
     8041 aggtgcctat gctcaatcaa gaactggaac tcagtttaaa tatggctttc gccagagcgc
     8101 gcgagcaccg tcatgagttt atgaccgtcg agcacttgtt actggcgctg ctcagtaacc
     8161 catctgcccg ggaggcgctg gaagcgtgtt ctgtggattt ggttgcgctc cgtcaggaac
     8221 tggaagcctt tattgaacaa accacacccg ttctgcctgc cagtgaagag gagcgcgaca
     8281 cacagccgac gctgagtttt cagcgtgtac tgcaacgtgc ggtcttccat gtccagtcct
     8341 ccggtcgcaa tgaggttacc ggtgcaaacg ttctggtcgc tatctttagc gaacaggagt
     8401 cgcaggcggc atatctgttg cgtaaacatg aagtcagccg tctcgatgtg gtgaacttta
     8461 tctctcatgg cacgcgtaaa gacgagccga cacagtcttc tgatcctggc agccagccaa
     8521 acagcgaaga acaagctggt ggggaggaac gtatggagaa tttcacgacg aacctgaatc
     8581 agcttgcgcg cgtgggcgga atcgacccac tgattggtcg tgagaaggag ctggagcgtg
     8641 ctattcaggt tctctgccgt cgccgtaaaa acaacccgct gctagtgggg gaatctggtg
     8701 tcggtaaaac cgcgattgca gaaggtcttg cctggcgaat tgttcagggc gatgtgccgg
     8761 aagtgatggc tgactgtacg atttactctc tcgatatcgg ttctctgtta gcgggcacaa
     8821 aatatcgcgg cgactttgaa aaacgtttta aagcgttgct caagcagctg gagcaggaca
     8881 ctaacagcat cctgtttatt gatgagatcc acaccattat cggtgcgggt gcagcgtctg
     8941 gtggtcaggt tgatgcggct aacctaatca aaccgttgct ctccagcggt aaaattcgcg
     9001 taattggttc gacaacctat caggagttca gcaacatttt cgagaaagac cgtgctctgg
     9061 cgcgtcgctt ccagaaaatt gatattactg aaccgtcgat cgaagaaact gttcaaatca
     9121 tcaatggcct gaaaccgaag tatgaagcac accacgacgt gcgttatacc gcaaaagcgg
     9181 tgcgtgcggc ggtagagctg gcggtgaaat acattaacga tcgtcatctg ccggataaag
     9241 ccattgatgt tatcgacgaa gcgggcgctc gcgcacgtct gatgccggta agcaaacgca
     9301 agaaaaccgt taatgtggcg gatattgagt ccgtggtggc ccgtattgca cgcattccag
     9361 agaagagtgt ttctcagagt gaccgcgata ccctgaaaaa cctcggcgat cgcctgaaaa
     9421 tgctggtctt cggtcaggat aaagccattg aggcgctgac tgaagccatt aagatggcgc
     9481 gtgcaggttt aggtcacgaa cataaaccgg tcggttcgtt cctgtttgcc ggccctaccg
     9541 gggtcgggaa aacagaggtg acggtacagc tttcgaaagc tttgggcatt gagcttctgc
     9601 gctttgatat gtccgagtat atggaacgcc ataccgtcag ccgtcttatt ggtgcgcctc
     9661 cgggatacgt tggttttgat cagggcggtt tgctgactga tgcggtcatc aagcatccac
     9721 atgcggtgct gctgctggac gaaatcgaga aagcgcaccc ggacgtgttc aatattctgt
     9781 tgcaggtgat ggacaacggt acgctgaccg ataacaacgg acgcaaagcg gacttccgta
     9841 acgtggtact ggtgatgacc actaacgccg gggtacggga aactgagcgc aaatccattg
     9901 gtcttatcca ccaggataac agcaccgatg cgatggaaga gatcaagaag atctttacac
     9961 cggaattccg taaccgtctc gacaacatta tctggttcga tcatctgtca actgacgtga
    10021 tccatcaggt ggtggataaa ttcatcgtcg agttgcaggt tcagctggat cagaaaggtg
    10081 tttctctgga agtgagccag gaagcgcgta actggctggc cgagaaaggt tacgaccggg
    10141 caatgggcgc acgtccgatg gcgcgtgtca tccaggacaa cctgaaaaaa ccgctcgcca
    10201 acgaactgct gtttggttcg ctggtggacg gcggtcaggt caccgtcgcg ctggataaag
    10261 agaaaaatga gctgacttac ggattccaga gtgcacaaaa gcacaaggcg gaagcagcgc
    10321 attaatctga ttgtcaggta ggttggtgaa gtccgtaatc tcgaaagagg ttacggactt
    10381 tttatttatg gggtggaacg gtgaaaaccc tatttttgga ggtgaaggta atttgttgat
    10441 aattagtgct gctggaaaag gtaaggataa aaaagggtga cagcaggaaa atgagatggt
    10501 tttgctttat taacaacggg ctaaacgtgt agtatttgag ttcactgccg tacaggcagc
    10561 ttagaaattc actggtaaca tactccaccc gcccaccatg ttcactgccg tacaggcaga
    10621 taaaatgcga aaaaaaagcc cgtactttcg tacgagctct tctttaaata tggcggtgag
    10681 ggggggattc gaacccccga tacgttgccg tatacacact ttccaggcgt gctccttcag
    10741 ccactcggac acctcaccaa attgttttgc tgccaaacct catgggtggc aacggggcgc
    10801 tactataggg agttggagta aaacggtcaa gaagaatttt aatgataatt attgtttgct
    10861 catactgtaa acaacttgtg cagtatatct acatcgagac aagttatgga cttatacttc
    10921 caaagtactt catgcatatc acaaaataaa aaggccggtt aaaccgacct tttactcgtt
    10981 ctttctcttc gcccatcagg cggtaaaaca atcagcgact acggaagaca atgcggcctt
    11041 tgctcaggtc gtacggggtc agttcaacag tcactttgtc gcccgtcagg atgcggatgt
    11101 agtttttgcg cattttaccg gagatgtgtg cagtaaccac gtgaccgttt tctaactcta
    11161 cgcggaacat ggtattaggc aacgtttcaa gaacggtacc ttgcatttca atattgtctt
    11221 ctttggccat ctaatcctct ggggtatcac taccgtaatt tgaaccggca agataatgcc
    11281 gaagttctgt aaataagtaa agatttgcgc gctaaatcgc aacaaacagg ttcggcacat
    11341 tactccgaaa acacacggct aagccgcacc aaaagcgcaa cgtataaggg agcggtgaga
    11401 taaacgatgg gcgttacctg acgcgaaaaa ttccttatcg gcagcggggt aatgagcgta
    11461 accaactctg cgaccgcaat tataacactc tggggagaaa tgtgccgaaa acattcattc
    11521 ttgtggtgaa aacaagcatc gtggtaccca gaaattattc ggcaatcgtc cgaggcgcat
    11581 ttgattgaga taattaaggt aattccggcg gggaatttcg caggcaccaa gcgatgctgt
    11641 gtgatcgtta aggacctggc agtcgataag cttaccgcca tgaccgataa attcctcaca
    11701 gaataccaga agcgccgttt tagacgcatt ttccatccgg ctgaacatgg actcgccaca
    11761 aaatagcgtt ccctgggcca cgccgtacat accgccgaca agctcatctt cacgccagac
    11821 ttcaatggag tgggcatgac cgagttcgtg aaggcgatgg taggcttcga ccacgccacg
    11881 cgtgatccag gttccttctt cgcgatcgct ggcacagcct tcaatgacct gaccaaaagc
    11941 gtaattcatc gtgacacgat agggcgagcg tttatgaaat cgcttcatac tacggctgat
    12001 atgcagtgat tctggccata gcaccgcgcg gggatcgggc gaccaccaga ggatggggtc
    12061 gcctggagaa aaccacggaa aaataccacg ctggtaagcc attaacaggc gcgcagggct
    12121 aagatcgccc ccaagtgcca gcaggccgtt aggctcacgt aatgcgcctt ccggggaagg
    12181 gaaggctatt gaatggcgag aaagctgaac caggcgcatg accgcaaaac tccacgcaag
    12241 tcggatcgtt caataatagc ttacaaaccc tgcttgaact ggtaataacg cccctgtctg
    12301 gcaagcagtt ctgcgtgagt accttgctca ataatttgcc cgttgtccat cactattatt
    12361 tgttggaaac gagagagtcc gcgaagtcga tgggtgacca ttaacaccgt tttctcacgc
    12421 atcatttctg caagcaattc aaggatctgg ctttcggttg tggcatctaa gccttcggta
    12481 ggttcatcca gcaacaccag tggcgcatca tgtaacagcg cacgggcgat agccagacgg
    12541 cgcagttcac caccggagag ctggcgtccg ccttcaccta accaactgtt gagacctgca
    12601 tcctcgagct gcttctccag accaacgcga cgtaagatca ccgccagagc ctcatcacta
    12661 ctgccaggcg aggcgagtaa cagattatca cgcagcgtgg cgctaaacag atgcactcgc
    12721 tgaggaacaa cgctgatggt ctgtcgtaga gccgcttcat tcaggctggc tatggggcta
    12781 tcgttaagca aaatctcgcc ctgttgcggg tcccatgcgc gggtcagcag ttgtaacagt
    12841 gttgatttgc cgcatccggt tcgcccgaga atcgctatat gttccccggc gtttacctga
    12901 agagaaatcc ctttaagtgc ctgttgggat tgctccggat aagtgaactg aacatcccgt
    12961 aatgtcagcg aaacgcgatc ggcaacacga gtttgggtat cagggaaggt gacctccggt
    13021 ttttgatccg ttaagtcaga gatacgtacg gcagaggcaa tgacttgccc cagatgctga
    13081 aatgcacccg ttactggtgc cagtgcttca aacgcggcta acgcgcaaaa gacaaacagg
    13141 gcaattaacg cgccgggttg agcattgccg ccaacgccgc cagacgccat ccacagcatc
    13201 aggatcaccg ctaacgcgcc aatgagcagc attatcgctt gcgacaatgc ggtcagttca
    13261 gattgacggc gttgcgcttc cagccattga atttctgtat tctctagttg cgtgcgataa
    13321 cgatcgctgg caccaaaaat ggtcagctca gcttgccctt gcagccaggc cgtcagttgt
    13381 tggcgatact gtccgcgaag atgagtcaga ttttgcccgg tgctttttcc cgcacgataa
    13441 aacagcggtg gcatcaggaa aagcgtcagt aacataatgc cgcccagcgt aaaggcgagg
    13501 gtggaatcaa ggaaacttaa cccgattgtc accaccataa tcaccacaaa agcgcccacc
    13561 agcggcgaga taacgcgcag gtaaagatga tcgagcgtat caacatccgc caccacgcgg
    13621 ttgagcaatt cgccctgacg atagcgcgcc agtccggcag gggagagggg caacaatttg
    13681 ctgaaggtgt aaatgcgcag atgctgcaac acgcggaaag tcgcgtcgtg acttaccaga
    13741 cgttcaaaat aacgcccggc agtacgggtg attgctgcac cacgcacgcc cgcagcgggt
    13801 agcatatagt tgaagctgta cagtccggca acccccgcaa ccgctgaggc cgagaggaac
    13861 cagccggaaa gtgtcaacag accgatactg gcgagcagcg tcacaattgc cagcacaata
    13921 ccaagactta acatccattt atgacgttta tacagtgcca gatagggtag caaagcgcgc
    13981 atttaaatct cctcctgacg atgggccagt aatgtggcga atgggccacc agccacactt
    14041 aattccgcgt aacgtccttg ctcaataatc cggccatcct gcataaccca aatgacatcc
    14101 cagtcagcaa gatcttctaa ctggtgggtg accattaacg ttgtctggcg cagagaggcg
    14161 gcattcagcg cctccattac gcgctgttca ctgtgagcat caaggctggc agcgggttca
    14221 tccaacagta atagcgaaca gggatttagt aacgcacggg ccaccgccac gcgctgcgcc
    14281 tgccccacgg aaaggcgggc agcctggtcg ccaacaggcg tatcaacgcc ttgtggcagg
    14341 agcggtagaa actcgctgac ccaggcgtta tccagcgctg cttgtaattc ttgttcgctg
    14401 gcatcaggtc gcgccagtag tacgttatcc cgtaatgttg ctgccggtaa ttgtgggttt
    14461 tgaccaaccc aggagagatg tttacgccat gattctggtg ataaatcgcg taattctatc
    14521 ccgttgattc gtaatgatcc ctgatatgag agaaaaccag aaagcgcgtt cagcagtgag
    14581 cttttacctg aaccgctgcg accaaccaac actgcacgtt ggcctgctgg caaagtaaag
    14641 ttcagcggtc cggccagcgt tttaccttcc ggcgacgtga taaacagatc ctcggcctca
    14701 atggtcagcg gatcggttga tgctaattcc gtctcaccgc gctgcggatg ggcgagcggg
    14761 gtttccataa acgttttcag actgtcagct gcaccaacag cctgggcttt agcatgataa
    14821 aacgtaccga gatcgcgtaa tggctggaaa aactctggcg caaggatcag ggccagaaaa
    14881 cccgcagcca gcgtcacgcc agtatcgtag tgaccaaaat ccagctcgcc gagataggaa
    14941 aaaccaaagt agaccgccac cagagcaatt gacagcgagg taaaaaattc gagaatgccg
    15001 gaggataaaa acgccagccg tagcacttcc attgtccgtt ggcggaaatc ttccgaagca
    15061 gaacgaatac tttcaatttc agcttcacca cgaccaaaaa tacgcaatgt ttccatgccg
    15121 cgcaggcgat cgaggaaatg cccacttaag cgagcaagag cgagaaagtt acgtcggtta
    15181 gcatcggcag cccccatccc aaccagcgcc ataaacaacg gaattaacgg tgcagtgccc
    15241 agcagaatga gcgccgcagc ccagttagag gggaagatcg ccaccacaat cagcaacggc
    15301 accgacactg ccagcgccat ttgcggcagg tagcgtgcat agtaatcatg catatcgtca
    15361 atttgctcga gtaccagcgt cgcccagctc cccgcaggtt taccctgaat ccacgctggc
    15421 cctgcttgtt gcagacggtc gagaacctga cggcggatgg caaagcggat atgctgcccg
    15481 gcgtgataac ccacccgttc gcgtaaccag accacccatg cgcgcagtac aaaggtcaga
    15541 accagtaacg taaagggaag cagcagggct tcacggggaa tattctccat aatcatatgt
    15601 tgcagaattc gcgccatgaa ccatgcctgg gcaatgatca atatgccgct cacaaagccc
    15661 agcagacgag aaatattcag ccaacgttgg gagatgacgc tttgctgttt taaccagcgg
    15721 gttaactctt tttgacgaga tttattcatt gcacgcttag caggtgagtt atcagaatta
    15781 tttgcagagc aatgttacaa cggggaaaaa ataaaggcga cccatagtcg catggtgtcg
    15841 ccttctttac ttttgttact gatttgtaaa attattttgc gtcagctaaa ccatcgaggt
    15901 agcgttccgc atcaagtgct gccatgcagc ctgtaccagc agatgtaatg gcctggcgat
    15961 aaatgtgatc catcacgtcg cctgcggcaa agacgccagg aatgctggtc tgggtggcat
    16021 taccatgaat acccgactgc actttgatgt agccgttttc cagttccagc tgcccttcga
    16081 aaatggcagt attcgggctg tgaccgatag caacaaacag accggcaacg tcgagtgact
    16141 cgatgttatc gctgttttgc gtatcgcgca gacgaacgcc agtgacaccc atttgatcgc
    16201 cggtcacttc ttccagcgta cggttggtgt gcagaatgat gttgccgttc tccactttat
    16261 ccatcagacg cttaatgagg attttttccg cgcggaaacc gtcacggcgg tgaatcagat
    16321 gcacttccga agcgatgtta gacagataca gcgcctcttc aaccgcggta ttgccgccgc
    16381 cgatgaccgc aactttctgg ttgcgataga agaaaccgtc gcaggttgca caagcagaaa
    16441 ctccacggcc tttaaaggct tcttcagagg gcaggccgag atagcgtgca gaagctccgg
    16501 tggcaataat cagcgcgtcg caagtgtatt cgccgttatc gccattcaga cggaacggac
    16561 ggttttgcag atccaccttg ttgatatgat caaaaatgat ctcagtttca aacttggtgg
    16621 catgttcgtg catgcgctcc attaataacg gaccggtcag atcgtttgga tcgccaggcc
    16681 agttttccac ttccgtggtg gtggtcagtt ggccgccttt ttccatgccg gtaatcagca
    16741 caggttgcag gttggcgcgc gccgcataga cagcagcggt gtatcccgcc gggcctgaac
    16801 ccaggataag cagtttactg tgtttggtcg tgcccatgag atccccatag ttgttggcag
    16861 acaatgggca ggattgtagg gaatttacag acgtaaaaaa agagtatgac gattttgtta
    16921 acaatttgtg caatcggcag catcgataag caggtcaaat tctcccgtca ttatcacctc
    16981 tgctacttaa atttcccgct ttataagccg attaaatgat gaataaacgc ccctgttaat
    17041 gaatatctgg catgttgtac taaaaatcga tgttttgctt tgacaatccc ctggtgtttt
    17101 gcgaaaacat tcgaggaaga aaaaaaacag tattcttata tgcgcataac catgcatgta
    17161 aataccatgt ttaccgtgct agtgaaatct acgtatggcg tggacagacg ccattcgtga
    17221 tgtcgatagc tgccacaagg caacggtctt ctcaccgtag acccaggcat tgcgcgccgt
    17281 gaatcttcat gatttcggtc tatcgtgacg ggtagcgact ctgaacagtg atgtttcagg
    17341 gtcagacagg agtagggaag gaatacagag agacaataat aatggtagat agcaagaagc
    17401 gccctggcaa agatctcgac cgtatcgatc gtaacattct taatgagttg caaaaggatg
    17461 ggcgtatttc taacgtcgag ctttctaaac gtgtgggact ttccccaacg ccgtgccttg
    17521 agcgtgtgcg tcggctggaa agacaagggt ttattcaggg ctatacggcg ctgcttaacc
    17581 cccattatct ggatgcatca cttctggtat tcgttgagat tactctgaat cgtggcgcac
    17641 cggatgtgtt tgaacaattc aataccgctg tacaaaaact tgaagaaatt caggagtgtc
    17701 atttagtatc tggtgatttc gactacctgt tgaaaacacg cgtgccggat atgtcagcct
    17761 accgtaagtt gctgggggaa accctgctgc gtctgcctgg cgtcaatgac acacggacat
    17821 acgttgttat ggaagaagtc aagcagagta atcgtctggt tattaagacg cgctaacacg
    17881 gaacaggtgc aaaatcggcg tattttgatt acactcctgt taatccatac agcaacagta
    17941 ctggggtaac ctggtactgt tgtccgtttt agcatcgggc aggaaaagcc tgtaacctgg
    18001 agagcctttc ttgagccagg aatacactga agacaaagaa gtcacattga caaagttaag
    18061 tagcggccgc cgccttctgg aggcgttgct gatccttatt gtcctgtttg ccgtctggtt
    18121 gatggctgcc ttactaagct ttaacccttc ggaccccagc tggtcgcaaa cggcctggca
    18181 tgaacctatc cataatttag gtgggatgcc cggtgcgtgg ttggcagata cgctgttctt
    18241 tatttttggc gtgatggctt acaccattcc cgtcattatt gtcggcggtt gttggtttgc
    18301 ctggcgtcat cagtccagcg acgaaaacat tgattatttt gccgtttcgc tacgcatcat
    18361 tggcgttttg gcgctcatcc ttacctcctg tggtctggcg gcaatcaacg ctgacgatat
    18421 ctggtatttt gcctccggtg gcgtcattgg cagcttacta agcactacgc tacaaccact
    18481 gctacacagt agcgggggaa ctattgcgct gctctgcgtt tgggcagcgg gcctgacgtt
    18541 gttcaccggt tggtcatggg tgaccattgc tgaaaaactc ggcggctgga ttttaaacat
    18601 tctcaccttc gccagtaatc gtacccgtcg cgatgatacc tgggtcgatg aagatgagta
    18661 tgaagacgac gaagagtatg aagatgaaaa ccacggcaaa cagcatgaat cacgccgtgc
    18721 ccgtattctt cgcggcgcgc tagcgcgtcg taaacggttg gcggaaaaat tcattaatcc
    18781 gatggggcgg caaacagacg ctgcgttgtt ctccggtaag cggatggatg atgacgaaga
    18841 gattacctac actgcacgcg gtgtggctgc cgacccggac gacgtcctat tttcgggcaa
    18901 tcgtgcaacg cagccagaat atgacgaata cgatccatta ttaaacggtg cgccaattac
    18961 cgaacctgtc gctgtagcag ctgctgctac cacggcgaca caaagctggg ctgcgccggt
    19021 tgaacctgtg actcagacgc cgcctgttgc ctctgttgat gttccacctt cgcaacctac
    19081 agtagcctgg cagcctgtac cgggtccaca aacgggagag ccggttattg ctcctgcacc
    19141 ggaaggttac ccacagcagt cacaatatgc gcagcctgca gtgcaatata atgagccgct
    19201 gcaacaacca gtacagccgc agcagccgta ttatgcacct gcagctgaac aacctgcgca
    19261 acagccgtat tatgcccctg cgccagaaca accggtggca ggtaacgcct ggcaagccga
    19321 agagcagcaa tccacttttg ctccacagtc tacataccag actgagcaaa cttatcagca
    19381 gccagccgct caggagccgt tgtaccaaca gccgcaaccc gttgaacagc agcctgttgt
    19441 ggagcctgaa cccgttgtag aagagacaaa acccgcgcgt ccgccgcttt actactttga
    19501 agaagttgaa gagaagcgag cccgtgaacg tgaacaactt gcggcctggt atcaaccgat
    19561 tccagaaccg gttaaagaac cagaaccgat caaatcttcg ctgaaagcac cttctgttgc
    19621 agcagtacct ccagtagaag ccgctgccgc tgtttctccg ctggcatctg gcgtgaaaaa
    19681 agcgacactg gcgacggggg ctgccgcaac cgttgccgcg ccagtcttca gtctggcaaa
    19741 tagcggtgga ccgcgtccgc aggtcaaaga ggggattggc ccgcagttgc cacgaccgaa
    19801 acgtatccgc gtgccaactc gtcgtgaatt ggcgtcttac ggtattaagc tgccctcaca
    19861 gcgtgcggcg gaagaaaaag cccgtgaagc ccagcgcaat cagtacgatt ctggcgatca
    19921 gtacaacgat gatgaaatcg atgcgatgca gcaggatgaa ctggcacgtc agttcgccca
    19981 gacacagcag caacgctatg gcgaacagta tcaacatgat gtgcccgtaa atgcagaaga
    20041 tgcagatgct gcggcagagg ctgaactggc tcgtcagttt gcgcaaactc aacaacaacg
    20101 ttattccggc gaacaaccgg ctggggcgaa tccgttctcg ctggatgatt ttgaattttc
    20161 gccaatgaaa gcgttgctgg atgatggtcc acacgaaccg ctgtttacgc caattgttga
    20221 acctgtacag cagccgcaac aaccggttgc accgcagcag cagtatcagc agccacaaca
    20281 gccggttgcg ccacagccgc agtatcagca gccacaacag caggttgcgc cgcagccaca
    20341 atatcagcag ccgcaacaac cggttgcgcc acagcagcaa tatcagcagc cgcaacaacc
    20401 agttgcgccg cagccgcagt atcaacagcc gcaacaaccg gttgcaccgc agccgcagta
    20461 tcagcagcca caacagccag ttgcgccaca gccgcaggat actctgcttc atccgctgtt
    20521 gatgcgtaat ggcgacagcc gtccgttgca taaaccgacg acgccgctgc cttctctgga
    20581 tttgctgaca ccgccgccga gcgaagtgga gccggtagat acctttgcgc ttgaacaaat
    20641 ggctcgcctg gtggaagcgc gtctggctga tttccgtatt aaagccgatg tcgtcaatta
    20701 ctctccgggg ccggttatca ctcgctttga acttaacctg gcaccgggcg taaaagcggc
    20761 gcgcatttct aacttgtcac gggaccttgc ccgttcactt tcgacggtgg cggtgcgtgt
    20821 cgttgaagtt attcctggca aaccctatgt aggtctggag ttaccgaata aaaaacgaca
    20881 aaccgtttat ctgcgcgaag tattggataa cgccaaattc cgcgataatc catcgccatt
    20941 aaccgtggtg ctgggtaaag atatcgccgg tgagccggtg gttgccgatc tggcgaaaat
    21001 gccgcacttg ttagttgcgg ggactaccgg ttccggtaaa tctgtcggtg tgaacgcgat
    21061 gatcctgagc atgctttata aagcacagcc agaagatgtg cgtttcatca tgatcgaccc
    21121 gaaaatgctg gagctttcgg tgtatgaagg cattccgcac ctgttaacgg aagtcgttac
    21181 tgatatgaaa gatgccgcca acgcgctgcg ctggtgtgtt aacgagatgg agcgtcgtta
    21241 taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt tataacgaaa aaattgctga
    21301 agccgatcgc atgatgcgtc cgattccaga cccgtactgg aagccgggtg acagtatgga
    21361 tgcccagcat ccggtgctga aaaaagaacc atacattgtg gtgttggtgg acgaatttgc
    21421 cgacctgatg atgacagtag gtaaaaaagt ggaagagctg atagcacgtc tggcgcaaaa
    21481 agcccgtgcc gcgggtatcc acctcgtact ggcaactcag cgtccatcgg ttgatgttat
    21541 tactggtctg attaaagcga atattccgac ccgtatcgcc tttaccgtat ccagtaagat
    21601 tgactcacgt accattcttg atcaggctgg cgcggaatca ctgctgggta tgggggatat
    21661 gctctactct gggccgaact ccacgttgcc ggtacgtgtc catggtgctt ttgttcgcga
    21721 tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt ggtcgcccac agtatgttga
    21781 tggcatcacc tccgacagcg aaagcgaagg tggtgcgggt ggtttcgatg gcgctgaaga
    21841 actggatccg ttgttcgatc aggcggtgca gtttgtcact gaaaaacgca aagcgtcaat
    21901 ttctggcgta caacgtcagt tccgcattgg ttataaccgt gcagcgcgta ttatcgaaca
    21961 gatggaagcg caggggattg tcagcgaaca ggggcacaac ggtaatcgtg aagtgctgtc
    22021 cccaccgccg tttgactaat taatgcattg ccggataagg cgcggtagcg tcgcatccgg
    22081 cactctatca actgaaaatt cagtattttc ttctttcctc aagctgatta ttagcctgga
    22141 atagagagta gagggaactc ccgatcggga gtgacgtaat ttgaggaata atgatgaaaa
    22201 aaattgccat cacctgtgca ttactctcaa gcttagtagc aagcagcgtt tgggctgatg
    22261 ccgcaagcga tctgaaaagc cgcctggata aagtcagcag cttccacgcc agcttcacac
    22321 aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat ctgtgggtga
    22381 aacgtccaaa cttattcaac tggcatatga cacaacctga tgaaagcatt ctggtttctg
    22441 acggtaaaac actgtggttc tataacccgt tcgttgagca agctacggca acctggctga
    22501 aagatgccac cggtaatacg ccgtttatgc tgattgcccg caaccagtcc agcgactggc
    22561 agcagtacaa tatcaaacag aatggcgatg actttgtcct gacgccgaaa gccagcaatg
    22621 gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg cacaatccat cagtttagcg
    22681 cggtggagca ggacgatcag cgcagcagtt atcaactgaa atcccagcaa aatggggctg
    22741 tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat gatcaacgta
    22801 agtagaggca cctgagtgag caatctgtcg ctcgattttt cggataatac ttttcaacct
    22861 ctggccgcgc gtatgcggcc agaaaattta gcacagtata tcggtcagca acatttgctg
    22921 gctgcgggga agccgttgcc gcgcgctatc gaagccgggc atttacattc tatgatcctc
    22981 tgggggccac cgggtaccgg caaaacaacc ctcgctgaag tgattgcccg ctatgcgaac
    23041 gctgatgtgg aacgtatttc tgccgtcacc tctggtgtga aagagattcg cgaggcgatc
    23101 gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta ttctttttgt tgacgaagtt
    23161 caccgtttca acaaaagcca gcaggatgca tttctgccac atattgaaga cggcaccatc
    23221 acttttattg gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc actgctttcc
    23281 cgtgcccgtg tttatctgtt gaaatccctg agtacagagg atattgagca agtactaact
    23341 caggcgatgg aagacaaaac ccgtggctat ggtggtcagg atattgttct gccagatgaa
    23401 acacgacgtg ccattgcaga actggtgaat ggcgacgcgc gccgggcgtt aaatacgctg
    23461 gaaatgatgg cggatatggc cgaagtcgat gatagcggta agcgggtcct gaagcctgaa
    23521 ttactgaccg aaatcgccgg tgaacgtagc gcccgctttg ataacaaagg cgatcgcttt
    23581 tacgatctga tttccgcact gcataagtcg gtacgtggta gcgcacccga tgcggcgctg
    23641 tactggtatg cgcgaattat taccgctggt ggcgatccgt tatatgtcgc gcgtcgctgt
    23701 ctggcgattg cgtctgaaga cgtcggtaat gccgatccac gggcgatgca ggtggcaatt
    23761 gcggcctggg attgctttac tcgcgttggc ccggcggaag gtgaacgcgc cattgctcag
    23821 gcgattgttt acctggcctg cgcgccaaaa agcaacgctg tctacactgc gtttaaagcc
    23881 gcgctggccg atgctcgcga acgcccggat tatgacgtgc cggttcattt gcgtaatgcg
    23941 ccgacgaaat taatgaagga aatgggctac gggcaggaat atcgttacgc tcatgatgaa
    24001 gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg aaatagcaca aacacgctat
    24061 tatttcccga caaacagggg ccttgaaggc aagattggcg aaaagctcgc ctggctggct
    24121 gaacaggatc aaaatagccc cataaaacgc taccgttaat gttatcgttg cggtaatgtt
    24181 gttactgtat ccctgtggtc gcaggctgtg gccacatctc ccatttaatt cgataagcac
    24241 aggataagca tgctcgatcc caatctgctg cgtaatgagc cagacgcagt cgctgaaaaa
    24301 ctggcacgcc ggggctttaa gctggatgta gataagctgg gcgctcttga agagcgtcgt
    24361 aaagtattgc aggtcaaaac ggaaaacctg caagcggagc gtaactcccg atcgaaatcc
    24421 attggccagg cgaaagcgcg cggggaagat atcgagcctt tacgtctgga agtgaacaaa
    24481 ctgggcgaag agctggatgc agcaaaagcc gagctggatg ctttacaggc tgaaattcgc
    24541 gatatcgcgc tgaccatccc taacctgcct gcagatgaag tgccggtagg taaagacgaa
    24601 aatgacaacg ttgaagtcag ccgctggggt accccgcgtg agtttgactt tgaagttcgt
    24661 gaccacgtga cgctgggtga aatgcactct ggcctcgact ttgcagctgc agttaagctg
    24721 actggttccc gctttgtggt aatgaaaggg cagattgctc gcatgcaccg cgcactgtcg
    24781 cagtttatgc tggatctgca taccgaacag catggctaca gtgagaacta tgttccgtac
    24841 ctggttaacc aggacacgct gtacggtacg ggtcagctgc cgaaatttgc tggcgatctg
    24901 ttccatactc gtccgctgga agaagaagca gacaccagta actatgcgct gatcccaact
    24961 gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca tcgatgaaga tgatctgcca
    25021 attaagatga ccgcccacac cccatgcttc cgttctgaag ccggttcata tggtcgtgac
    25081 acccgtggtc tgatccgtat gcaccagttc gacaaagttg aaatggtgca gatcgtgcgc
    25141 ccggaagact caatggcggc gctggaagag atgactggtc atgcagaaaa agtcctgcag
    25201 ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg gcgacatggg ctttggcgct
    25261 tgcaaaactt acgacctgga agtatggatc ccggcacaga acacctaccg tgagatctct
    25321 tcctgctcca acgtttggga tttccaggca cgtcgtatgc aggcacgttg ccgcagcaag
    25381 tcggacaaga aaacccgtct ggttcatacc ctgaacggtt ctggtctggc tgttggtcgt
    25441 acgctggttg cagtaatgga aaactatcag caagctgatg gtcgtattga agtaccagaa
    25501 gttctgcgtc cgtatatgaa cggactggaa tatattggct aatacccaat ttttctgaat
    25561 ctaaaaagcg cctacgggcg ctttttttgt ctccctttga taccgaacaa taattactcc
    25621 tcacttacac gtaatactac tttcgagtga aaatctacct atctctttga ttttcaaatt
    25681 attcgatgta tacaagccta tatagcgatc tgctatagaa ataattacac aatacggttt
    25741 gttactggaa tcaatcgtga gcaagcttga gtgagccatt atgaaaacga aaatccctga
    25801 tgcggtattg gctgctgagg tgagtcgccg tggtttggta aaaacgacag cgatcggcgg
    25861 cctggcaatg gccagcagcg cattaacatt accttttagt cggattgcgc acgctgtcga
    25921 tagcgccatt ccaacaaaat caggcgaaaa ggttatctgg agcgcctgta cagttaactg
    25981 tggtagtcgc tgcccgctac gtatgcacgt cgtggacggt gaaatcaaat atgtcgaaac
    26041 ggacaatacc ggcgatgaca attacgacgg cctgcaccag gttcgcgcct gcctgcgtgg
    26101 gcgttccatg cgtcgccgtg tctacaatcc ggaccgcctg aaatatccga tgaaacgagt
    26161 cggggcgcgc ggtgaaggca aattcgagcg cattagctgg gaagaagcct acgacatcat
    26221 cgcgaccaat atgcagcgcc tgatcaaaga gtacggcaac gaatccatct atctgaacta
    26281 tggcaccggt acgctgggtg gcaccatgac ccgctcctgg ccgccgggaa ataccctggt
    26341 cgcgcggctg atgaactgct gcggcggcta tctgaaccat tacggcgact actcctccgc
    26401 gcaaattgcg gaaggtctga actataccta cggcggctgg gcagatggca acagcccgtc
    26461 ggatatcgaa aacagtaagc tggtagtgct gtttggtaat aaccctggcg aaacgcgaat
    26521 gagtggcggt ggggtgactt actatcttga acaggcacgc cagaaatcta atgcccgcat
    26581 gatcatcatc gatccgcgct ataccgacac cggtgccggg cgcgaagatg agtggatccc
    26641 tattcgtccg ggaacagatg ccgcactggt taacggtctg gcgtacgtca tgatcactga
    26701 aaacctggtg gatcaggcat tcctcgataa atattgcgtt ggctacgatg agaaaaccct
    26761 gccagccagt gcgccgaaaa atggccacta taaagcttat attctgggtg aagggccaga
    26821 tggcgtggct aaaacaccgg aatgggcctc gcaaatcacc ggtgttccgg cagacaaaat
    26881 catcaaactg gctcgtgaaa tcggcagtac caaaccggcg tttatcagcc agggatgggg
    26941 cccgcagcgt cacgctaacg gtgaaatcgc aacccgtgct atctcgatgc tggcgattct
    27001 gaccggtaac gttggtatta atggagggaa cagcggcgcg cgtgaaggtt catacagttt
    27061 accgtttgtc cgtatgccga ccttggaaaa cccgatccag accagcattt cgatgtttat
    27121 gtggaccgat gccattgaac gtggcccgga aatgacggcg ctacgtgatg gtgtgcgcgg
    27181 gaaagataag ctggatgtgc cgatcaaaat gatctggaac tatgccggta actgcctgat
    27241 taaccagcat tctgaaatca accgtaccca tgaaatcctt caggatgata agaagtgcga
    27301 gctgattgtg gttatcgact gccacatgac ctcatcggcg aaatatgctg acatcctgct
    27361 gcctgactgc accgcttccg aacagatgga ctttgcactg gatgcatcct gcgggaatat
    27421 gtcttacgtg attttcaacg atcaggtgat taaaccgcgc tttgaatgta agaccatcta
    27481 tgaaatgacc agcgaactgg caaaacgtct tggcgttgag caacagttta ctgaaggccg
    27541 tacccaggaa gagtggatgc ggcatctgta tgcccagtcg cgggaagcga ttcctgaact
    27601 gccaacgttt gaagagttcc gcaagcaggg gatctttaaa aagcgcgacc cacaagggca
    27661 tcacgttgct tataaagcct tccgtgaaga tccgcaggca aatccactga ccacgccatc
    27721 gggtaaaatt gagatttatt cgcaggcgct ggctgacatt gccgctacct gggaattgcc
    27781 agaaggcgat gtgatcgatc cactgccgat ctacacgccg ggctttgaaa gttatcagga
    27841 tccgctgaac aaacagtatc cgctgcagct tacaggtttc cactataaat ctcgcgttca
    27901 ctcaacttac ggcaacgttg atgtgctgaa agcggcttgc cgtcaggaaa tgtggatcaa
    27961 cccgcttgat gcccaaaaac gcggtatcca caacggcgat aaagtcagga tctttaacga
    28021 tcgtggtgag gttcatattg aggcgaaagt gacgccacga atgatgccgg gtgtggtcgc
    28081 actgggtgaa ggtgcctggt atgacccgga tgcaaaacgt gtcgataagg gtggttgtat
    28141 taacgtactg accactcaac gtccgtctcc tctcgctaag gggaatccgt cacatacaaa
    28201 ccttgttcag gttgaaaagg tgtaaggagt aaccgatgac aacccagtat ggatttttta
    28261 ttgattccag ccgttgcacc ggttgcaaaa cctgcgagct ggcttgtaaa gactacaaag
    28321 atttgatgcc agaagtcagc ttccgccgca tttatgaata tgctggtggc gactggcagg
    28381 aagataacgg tgtctggcac cagaacgtgt ttgcctacta tctgtcgatt tcgtgcaacc
    28441 actgcgaaga tccggcttgt actaaagtct gcccgagcgg tgcgatgcat aaacgtgaag
    28501 atggttttgt tgtggtcgat gaagatgtgt gcattggctg ccgctactgc catatggctt
    28561 gcccgtatgg cgcaccgcaa tataacgaaa cgaaaggcca tatgaccaaa tgcgatggtt
    28621 gttatgaccg tgttgcggag ggtaaaaagc cgatctgtgt tgaatcttgt ccgctgcggg
    28681 cgctggattt cggccctatc gacgagctgc gtaaaaaaca tggcgatctg gcggccgttg
    28741 cgccgttgcc gcgagctcac tttaccaaac cgaatattgt gatcaaaccc aatgccaata
    28801 gccgcccgac cggggatacc actggctatc tggcaaaccc gaaggaggtg tgagatggga
    28861 agtggatggc atgaatggcc gctgatgatc ttcacggtct tcgggcaatg tgtggcgggt
    28921 ggttttatcg ttctggcttt ggcgctgctt aaaggcgacc tgcgagcaga agcccagcag
    28981 cgtgttatcg cctgcatgtt tggcttatgg gtgctgatgg gcattggctt tatcgcctct
    29041 atgctccatc ttggttcacc gatgcgcgcc tttaactcgc tcaaccgggt aggggcttca
    29101 gcactcagta acgaaatcgc cagcggttcg atcttttttg ctgtaggcgg catcggctgg
    29161 ttgctggcaa tgctgaaaaa gctgtcacct gcattgcgta cgctatggct ggtggtgacg
    29221 atggttcttg gcgtcgtctt tgtctggatg atggtgcgtg tgtataacag cattgatacc
    29281 gttccgacct ggtacagcat ctggacgccg atgggcttct tcctgacgat gttcatgggc
    29341 ggcccgttac tgggttacct gttgctgagt ctggctggcg tcgatggctg ggcgatgcgt
    29401 ctgctgccag ccatttctgt actggcactg gtggtgagtg gcgtgatgtc agtgatgcag
    29461 ggcacagagc tggcaactat tcatagttct gtgcagcagg ccgcagcgct ggtgccggac
    29521 tatggtgcgc tgatgtcctg gcgaatcgtg cttttggccg ttgccctgtg cttgtggatt
    29581 gcaccacagc taaaaggtta tcagcctgcg gttccgctac tatccgtctc attcattctg
    29641 cttctggcag gggaattaat cggtcgcggc gtattctatg gtttgcatat gaccgtgggg
    29701 atggccgtcg caagctaatc agtaacaacc ggggtttcgg ccccggttct cttttatttc
    29761 tgcttcgtta acgtgtcata actggtcatc aaattacgat aatcaggaat gtggttagag
    29821 aacaacgtcg ccagtccttc aatatcatta cgccagtcac ggtgcagctc gcaagccacg
    29881 ccgaaccatg tcatcaattg cgcacctgct tgcgacatgc gatcccatgc cgaatgccgg
    29941 gtaatttcat taaaggtccc tgacgcgtca gtcacaacaa agacatcaaa cccttcttca
    30001 atggctgaaa gcgccgggaa tgccacgcaa acttcggtta ccacaccggc aataattaac
    30061 tgttttttac ctgtcgcttt gactgctttt acaaaatctt cgttatccca ggcgttaata
    30121 ttgccagggc gagcaatgta aggtgcatcg gggaattgtg ctttcagctc tggaactaat
    30181 gggccgttag gaccggtttc aaaactggtg gtaagaatag ttggtaagtt gaaatactta
    30241 gccaggtcac ccagcgccag cacgttattt ttaaacttat cgggttcgat atcccgtaca
    30301 agggaaagta aaccagcctg gtgatcaaca agcaaaacgg cagcatcatt tttatcaaga
    30361 cgaacatacg gtttggtcat cctcttctcc tttcgagagt cagtggtacg tctgaaatcg
    30421 gtcagacgcg atgcattgct ctgaaagcat agacgggaaa tatgagtttg ctgtgaccat
    30481 gaaatttttc gactgaacga agcgtatgaa atttgtgtta gatcaataaa aacaatcaga
    30541 tgagattaat catctcttca gcattagcgt tatgaatgag taatataaat cgataatacc
    30601 gccgctgcga tatgcgcgta ttttcaccca ttgacaatgt tttttgcggt ggcatgatgc
    30661 gcatgaaatt tgaacttcct cacggtttta attcatgtcc acgtataccc ggcctgtcat
    30721 gcttttgctg tctggcctgc ttttgttgac tctggcgatc gcggtgttaa atacactcgt
    30781 gccgctttgg ctcgcccagg aacacatgtc cacatggcag gtaggcgttg tcagctcatc
    30841 ctattttacc ggcaaccttg tcggtacatt gctgacaggg tatgtcatta aacgcattgg
    30901 ctttaaccgc agctattatc tggcctcctt catttttgcc gctggctgtg ccggccttgg
    30961 cctgatgatt ggtttctgga gctggttggc gtggcgtttt gtcgcgggcg tcggctgtgc
    31021 catgatttgg gtagtcgttg agagcgcgct gatgtgcagt gggacgtcac gtaaccgtgg
    31081 gcgtttgctt gctgcgtata tgatggttta ttacgtggga acatttttag gccagttact
    31141 ggtcagcaaa gtctcaaccg agctgatgag cgtattgccg tgggttacag gtttgacgtt
    31201 ggcagggatc ttaccgctgt tgtttacgcg tgtcctgaat cagcaggctg aaaaccatga
    31261 ttcgacgtca attacgtcaa tgctaaaact ccgtcaggcg cggcttggcg tgaatggctg
    31321 cattatctca ggaatcgtgc tgggatctct atatggcctg atgccgctgt acctcaatca
    31381 caaaggggtg agcaatgcca gcattggttt ctggatggcg gtactggtca gtgcgggtat
    31441 cctcggacaa tggccgattg gacgtctggc ggataagttt ggtcgactgc tggtgttgcg
    31501 tgttcaggtc tttgtcgtca ttctcggcag tatcgcgatg cttagccagg cggcgatggc
    31561 cccagcgtta ttcatcctcg gtgccgctgg ctttacgcta tatccggtgg cgatggcctg
    31621 ggcttgtgag aaagttgaac atcatcaact ggtggcgatg aaccaggcct tactgttgag
    31681 ctataccgtg ggaagtctgc ttggcccgtc atttaccgct atgctaatgc agaatttctc
    31741 cgataattta ttgtttatca tgatcgccag cgtatcgttt atctatttgc tgatgctgct
    31801 tcgcaacgcc ggtcatacgc cgaaacccgt tgctcacgtg taaatgaatt caagcaaagt
    31861 gtgaacttac tgtttcacac tctgcttttt tgtttcttct atctgacttg cttttttcca
    31921 aattttattc gtttaaaaat aaaatgtgca gcaggttata attttgcatt tcgctatttc
    31981 cgcacttctt atttgccgcg cataatccct cgttttacag atgccccttt aattttggcg
    32041 aaggatttgt ctatggctgg gaatgttcag gaaaaacagc tgcgatggta caacattgcg
    32101 ctgatgtctt ttatcactgt ctggggtttt ggcaacgttg ttaataacta tgccaaccag
    32161 gggctggtgg ttgttttttc atgggtgttt atctttgcgc tctatttcac accttatgca
    32221 ctaattgttg gtcagttagg ctcgaccttc aaagatggga agggcggggt cagtacctgg
    32281 attaaacaca cgatgggacc cggattggct tacctcgccg cgtggaccta ctgggtggtg
    32341 catattccct atctggcaca aaaaccccag gcaatactga ttgcgctcgg ttgggcgatg
    32401 aaaggcgacg gttcgctaat caaagaatat tcagtcgtag cgttacaggg gttaacgctg
    32461 gtgctgttta tcttctttat gtgggttgct tcacgcggta tgaaatcgct gaaaatcgtc
    32521 ggttctgtgg cagggattgc gatgtttgtt atgtcgctcc tgtatgtggc gatggcggta
    32581 accgcgcctg cgattactga agtgcatatt gcgaccacaa acattacctg ggaaacgttc
    32641 attcctcata tcgactttac ctacattacc actatttcaa tgctggtttt cgcggttggc
    32701 ggagctgaga agatttctcc ttacgttaat caaacgcgca acccaggaaa agaatttcca
    32761 aaagggatgt tatgcctggc ggtgatggtt gcggtttgtg ccattctggg ctcactggcg
    32821 atggggatga tgtttgactc gcgtaatatc ccggatgact taatgactaa cggtcagtat
    32881 tacgcctttc agaagctggg cgagtattac aacatgggta atactttaat ggtgatttac
    32941 gccattgcga ataccctggg acaagtagct gcgctggtat tctcgattga tgccccgctt
    33001 aaagtgctat taggcgatgc tgacagcaaa tatattccag ccagtttatg tcgtaccaac
    33061 gcttctggta cgcccgttaa tggctatttt ctgaccctgg tgctggtggc gatcctgatt
    33121 atgctgccga ctctcggcat cggtgatatg aacaatctct ataaatggct gttgaatctt
    33181 aactcggtag tgatgccgct gcgttattta tgggtatttg ttgcatttat tgcagtcgtt
    33241 cgcctggcgc agaaatatac accagagtat gtctttattc gtaataagcc tctggcaatg
    33301 accgtcggga tttggtgttt tgcctttact gcctttgcct gtttgacggg gatcttcccg
    33361 aaaatggaag ccttcactgc agagtggacc ttccagttgg cgctgaatgt tgcaacgccg
    33421 tttgtgctgg taggattagg actgatattc ccgctgctgg cgcgtaaagc gaatagtaaa
    33481 taattattgt ggtcggtgac gctgactctg gcagatctga cccgttttta cataatcgag
    33541 aaatgcgcgc aagccgcagg acatatactg gcggtttggg taatacattt ggaagctgtg
    33601 ctgtaagggc gttagaaata gagctctgaa ttatttatgc agcgcggaat aaaaaagcgg
    33661 caactcaata aagttgccgc tttacgggga aattagaaca ttaccttatg accgtactgc
    33721 tcaagaatac ctttcacgcg ttccatggtt tctttcttcg gtggtttaac accatcgagt
    33781 ttgtattctt cacccatcgc cacccatttg tgtttgccca gttcgtggta ggggagaagt
    33841 tcgattttct caacgttacc catatcacgg gtaaattcac cgaggcgatg cgctgaatcg
    33901 tcatcgtcag accagcctgg gacaacaacg tagcggatcc acaccttcac atttttattc
    33961 gccagatatt tggcgaactc cagcgtgcgg tggttggaaa ctccaaccag attttggtgg
    34021 atctcgtcgt tcatctgttt gagatcgagc attaccaggt cggttacttc cagcagttca
    34081 tcaatcaccg gatcgtaacg acgaacaaaa ccgttggtgt ccagacaggt atgaatgcct
    34141 tcttttttgc aggcgcggaa ccagtcacga acaaactcag cttgcaggat tgcttcaccg
    34201 ccggatgcgg taacgccgcc gccggaagcg ttcataaagt ggcgataggt caccacttcc
    34261 ttcatcaaat cttcaacggt aacttcttta ccgccatgcg tgtcccaggt gtcgcggtta
    34321 tgacaataca ggcagcgcat caggcagccc tggaaaaagg tgataaagcg aatacctggg
    34381 ccgtctacgg ttccacagga ttcaaaggag tgaatgcgac caataactga cattgcggtg
    34441 tttctccaga tgtggcccat ctgaggccgt gttggtgcgc agctcgaagg ctacgtcgag
    34501 tctgttttgg cagtcacctt aaagtataga tagctgacaa aaaaggctca cgtgctaaaa
    34561 aaggccccac tttcgtggag cctttattgt acgcttttta ctgtacgatt tcagtcaaat
    34621 ctaattacat agattgagtg aaggtacgag taataacgtc ctgctgctgt tctttagtca
    34681 gcgagttgaa acgtactgcg tagccagata cacggatggt cagctgcgga tatttttccg
    34741 gattttccat cgcgtcgagc agcatttcac ggttcatcac gttaacgttc aggtgctgac
    34801 caccttcgat ggatgcttcg tggtggaagt aaccatccat cagaccagcc aggttggtct
    34861 tacgaacttc gtcgtcttta cccagtgcgt tcggaacgat agagaaggtg taggagatac
    34921 catctttagc gtaagcaaac ggcagtttag caacggaagt cagagacgct acagcacctt
    34981 tctggtcacg accgtgcatc gggttagcac ccggtccgaa cggcgcgcca gcacgacgac
    35041 cgtctggggt gttacccgtt ttcttaccat acacaacgtt agaagtgatg gtcagaacag
    35101 actgagtcgg gatagcgtca cggtaggtgt gcagtttctg aattttcttc atgaaacgtt
    35161 ctaccaggtc aacagccagg tcatctacac gcggatcgtt gttaccaaac tgcgggtatt
    35221 cgccttcgat ttcgaagtcg atagccagac cgtcttcgtc acgaatcggt ttaactttcg
    35281 catatttgat tgcagacagg gagtcagcag caacggacag accagcgata ccacacgcca
    35341 tggtgcggat aacgtcacgg tcgtgcagcg ccatcagaga ggcttcgtag ctgtacttgt
    35401 cgtgcatgta gtgaatgatg ttcagtgcag tgatgtactg tttagccagc cagtccatga
    35461 agtgatccat gcgctccatc acttcatcgt agttcaggag atcgcctttg atcggttcag
    35521 acttcggacc aacctgcatt ttcagttttt cgtcaacgcc gccgttgatt gcgtacagca
    35581 tggttttcgc caggtttgca cgcgcaccga agaactgcat ttgtttacca acgatcatcg
    35641 ggcttacgca gcaagcgata gcgtagtcat cgttgttgaa gtccggacgc atcaggtcat
    35701 cgttctcata ctgcagagaa gaggtgtcga tggacacttt agcggcgaat ttcttgaagt
    35761 tcagcggcag tttttcagac cacagaatgg tcatgttcgg ttccggagac ggacccatgg
    35821 tgtacagggt gttcaggaaa cggaagctgt ttttggtaac cagggtacga ccgtcgaggc
    35881 ccataccacc gatagattcg gttgcccaga tcgggtcgcc agagaacagt tcatcgtact
    35941 ccggagtacg caggaagcga accatacgca gtttcatgac caggtggtca accatttcct
    36001 gcgcttcttg ttcggtgatc ttgccagctt tcaggtcacg ttcgatgtac acatccagga
    36061 aggtggaggt acgaccgaag gacattgcag caccgttctg agacttaaca gcagccaggt
    36121 agccgaagta agtccactgg atagcttcct gagcgttggt agccggacca gagatgtcgt
    36181 agccgtattt cgcagccatt tctttcatct gacccagagc gcggtgctgt tcagcgattt
    36241 cttcgcgcag acggatagtc tgttccaggt ttacgccgtt ttccagatca gcctgcagag
    36301 aggtgaactg agcgtatttg tctttcatca ggtagtcgat accgtacagc gcaacgcgac
    36361 ggtagtcacc gatgatacgg ccacggccat aagcatctgg cagaccggtc agaacaccgg
    36421 atttacggca acgcaggatg tccggagtgt aaacgtcgaa cacgccctgg ttgtgagttt
    36481 tacggtattc agtgaagatt tttttgatca tcgggtccag ttcgcggttg tacgctttgc
    36541 aggaaccttc gatcattttg ataccaccga acgggataag agcacgtttc agcggagctt
    36601 cagtctgcag accaacaact ttttccaacg ctttgttgat gtagccagcg tcgtgagagg
    36661 tgatggtgga agcaacagcg gtgtcgaagt caactggcgc gtgagtgcgg ttttccagtt
    36721 taacgccttc cattactttg tcccacaggg tggtggtcgc ttcagtagcg ccagccagga
    36781 aggactcgtc accctcgtac ggagtgtagt ttttctgaat gaagtcacgg acgtttactt
    36841 cattctgcca gtcacctttg gtaaaacctt cccaggctgt ggctaacttt tcattaagct
    36901 cggacatgta acacctacct tcttaagtgg attttttatt tactgcgtac ttcgacaacc
    36961 attaatggtg atcgttttca cgcaggtaaa tgacccagta tgtcaaccca accaacaaac
    37021 caccgccgat aatattaccg atcgtaaccg gaatcaggtt atcagtgatg aagttcatca
    37081 cggtcaggtg agaaaaattt tccggtgcag aaccgacagc ggtccagaat tccggagatg
    37141 cgaagtcgcg gattacaata cccatcggga tcataaacat gtttgcgata ctgtgctcaa
    37201 aaccgctggc aacaaacatc gcgaccggca gcaccatgat aaacgctttg tccatcaggc
    37261 tgcggccaga ataactcatc catactgcca gacataccat caggtttgcc aggataccaa
    37321 ggcagacggc ctcaataaaa gtatggtgca ctttgtggtc ggcggtttgt aggacgttta
    37381 gtccccattg accatttgcg gtcatatact cgccggaaag ccacattaaa agtacaaaca
    37441 gcagtgcgcc gaccaggttg ccaaaataga catttagcca gtttttagcc aactgacccc
    37501 aggtgatgcg cccactcgcc ttagcaacaa caatcaacac tgtggaagta aagagatcgg
    37561 ctccgcagac aacacaaaga atcagtccca gagagaagca aatgccgcca accagttttg
    37621 ccatgccgaa gggcattgtg cctgtgccag tggttgctgt gatatagaag acgaatgcga
    37681 ttgagatgaa aacaccggcg gtaatcgcca gatagaaagt cttaagcgga tgtttcgttg
    37741 ctttatagac acccgcctct tcggccactt tggccattgc agcaggaagt aaaagatcaa
    37801 aagggttgtc agctttcaca ctaactctct ctttattaag tcggcgacga gatactaaca
    37861 aagcattata gatgagaaat tgatatagat catatctcgc ctggcttata ggcccgtaac
    37921 tcgc
//
LOCUS       DAZFBR010000036        36377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFBR010000036 DAZFBR010000000
VERSION     DAZFBR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.036"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            129..2298
                     /locus_tag="ACHJZ2_002027"
                     /pseudo
     CDS             129..2298
                     /locus_tag="ACHJZ2_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(2448..3812)
                     /gene="mnmE"
                     /locus_tag="ACHJZ2_002028"
     CDS             complement(2448..3812)
                     /gene="mnmE"
                     /locus_tag="ACHJZ2_002028"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO4568144.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(3918..5564)
                     /gene="yidC"
                     /locus_tag="ACHJZ2_002029"
     CDS             complement(3918..5564)
                     /gene="yidC"
                     /locus_tag="ACHJZ2_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO4568145.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(5567..5824)
                     /gene="yidD"
                     /locus_tag="ACHJZ2_002030"
     CDS             complement(5567..5824)
                     /gene="yidD"
                     /locus_tag="ACHJZ2_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO4568146.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            complement(5788..6147)
                     /gene="rnpA"
                     /locus_tag="ACHJZ2_002031"
     CDS             complement(5788..6147)
                     /gene="rnpA"
                     /locus_tag="ACHJZ2_002031"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO4568147.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(6164..6304)
                     /gene="rpmH"
                     /locus_tag="ACHJZ2_002032"
     CDS             complement(6164..6304)
                     /gene="rpmH"
                     /locus_tag="ACHJZ2_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO4568148.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(6534..6614)
                     /locus_tag="ACHJZ2_002033"
     CDS             complement(6534..6614)
                     /locus_tag="ACHJZ2_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568149.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            6911..8314
                     /gene="dnaA"
                     /locus_tag="ACHJZ2_002034"
     CDS             6911..8314
                     /gene="dnaA"
                     /locus_tag="ACHJZ2_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO4568150.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            8319..9419
                     /gene="dnaN"
                     /locus_tag="ACHJZ2_002035"
     CDS             8319..9419
                     /gene="dnaN"
                     /locus_tag="ACHJZ2_002035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO4568151.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            9419..10492
                     /gene="recF"
                     /locus_tag="ACHJZ2_002036"
     CDS             9419..10492
                     /gene="recF"
                     /locus_tag="ACHJZ2_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO4568152.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            10521..12935
                     /gene="gyrB"
                     /locus_tag="ACHJZ2_002037"
     CDS             10521..12935
                     /gene="gyrB"
                     /locus_tag="ACHJZ2_002037"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO4568153.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(12959..13057)
                     /locus_tag="ACHJZ2_002038"
                     /pseudo
     CDS             complement(12959..13057)
                     /locus_tag="ACHJZ2_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13175..13573
                     /gene="yidB"
                     /locus_tag="ACHJZ2_002039"
     CDS             13175..13573
                     /gene="yidB"
                     /locus_tag="ACHJZ2_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO4568154.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            13688..14500
                     /gene="yidA"
                     /locus_tag="ACHJZ2_002040"
     CDS             13688..14500
                     /gene="yidA"
                     /locus_tag="ACHJZ2_002040"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO4568155.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(14546..15202)
                     /gene="yidX"
                     /locus_tag="ACHJZ2_002041"
     CDS             complement(14546..15202)
                     /gene="yidX"
                     /locus_tag="ACHJZ2_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO4568156.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            15338..15451
                     /locus_tag="ACHJZ2_002042"
                     /pseudo
     CDS             15338..15451
                     /locus_tag="ACHJZ2_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15448..16512)
                     /gene="cbrA"
                     /locus_tag="ACHJZ2_002043"
     CDS             complement(15448..16512)
                     /gene="cbrA"
                     /locus_tag="ACHJZ2_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO4568157.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            16613..17827
                     /gene="yidR"
                     /locus_tag="ACHJZ2_002044"
     CDS             16613..17827
                     /gene="yidR"
                     /locus_tag="ACHJZ2_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO4568158.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(17829..18161)
                     /gene="yidQ"
                     /locus_tag="ACHJZ2_002045"
     CDS             complement(17829..18161)
                     /gene="yidQ"
                     /locus_tag="ACHJZ2_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4568159.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    18401..18473
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            18467..18880
                     /gene="ibpA"
                     /locus_tag="ACHJZ2_002046"
     CDS             18467..18880
                     /gene="ibpA"
                     /locus_tag="ACHJZ2_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO4568160.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            18992..19420
                     /gene="ibpB"
                     /locus_tag="ACHJZ2_002047"
     CDS             18992..19420
                     /gene="ibpB"
                     /locus_tag="ACHJZ2_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO4568161.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            19616..21277
                     /gene="yidE"
                     /locus_tag="ACHJZ2_002048"
     CDS             19616..21277
                     /gene="yidE"
                     /locus_tag="ACHJZ2_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO4568162.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(21274..21990)
                     /gene="yidP"
                     /locus_tag="ACHJZ2_002049"
     CDS             complement(21274..21990)
                     /gene="yidP"
                     /locus_tag="ACHJZ2_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4568163.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            22286..23902
                     /locus_tag="ACHJZ2_002050"
     CDS             22286..23902
                     /locus_tag="ACHJZ2_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4568164.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23902..25224
                     /locus_tag="ACHJZ2_002051"
     CDS             23902..25224
                     /locus_tag="ACHJZ2_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFO4568165.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(25221..26114)
                     /gene="yidL"
                     /locus_tag="ACHJZ2_002052"
     CDS             complement(25221..26114)
                     /gene="yidL"
                     /locus_tag="ACHJZ2_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4568166.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            26281..27996
                     /gene="yidK"
                     /locus_tag="ACHJZ2_002053"
     CDS             26281..27996
                     /gene="yidK"
                     /locus_tag="ACHJZ2_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO4568167.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            27993..29486
                     /gene="yidJ"
                     /locus_tag="ACHJZ2_002054"
     CDS             27993..29486
                     /gene="yidJ"
                     /locus_tag="ACHJZ2_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4568168.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(29533..29982)
                     /gene="yidI"
                     /locus_tag="ACHJZ2_002055"
     CDS             complement(29533..29982)
                     /gene="yidI"
                     /locus_tag="ACHJZ2_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO4568169.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            30091..30438
                     /gene="yidH"
                     /locus_tag="ACHJZ2_002056"
     CDS             30091..30438
                     /gene="yidH"
                     /locus_tag="ACHJZ2_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO4568170.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            30428..30790
                     /gene="yidG"
                     /locus_tag="ACHJZ2_002057"
     CDS             30428..30790
                     /gene="yidG"
                     /locus_tag="ACHJZ2_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO4568171.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            30787..31284
                     /gene="yidF"
                     /locus_tag="ACHJZ2_002058"
     CDS             30787..31284
                     /gene="yidF"
                     /locus_tag="ACHJZ2_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO4568172.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(31292..32476)
                     /gene="emrD"
                     /locus_tag="ACHJZ2_002059"
     CDS             complement(31292..32476)
                     /gene="emrD"
                     /locus_tag="ACHJZ2_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO4568173.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            32558..32632
                     /gene="ysdE"
                     /locus_tag="ACHJZ2_002060"
     CDS             32558..32632
                     /gene="ysdE"
                     /locus_tag="ACHJZ2_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO4568174.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(32756..32845)
                     /gene="tisB"
                     /locus_tag="ACHJZ2_002061"
     CDS             complement(32756..32845)
                     /gene="tisB"
                     /locus_tag="ACHJZ2_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO4568175.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            33410..33508
                     /gene="ivbL"
                     /locus_tag="ACHJZ2_002062"
     CDS             33410..33508
                     /gene="ivbL"
                     /locus_tag="ACHJZ2_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO4568176.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            33614..35302
                     /gene="ilvB"
                     /locus_tag="ACHJZ2_002063"
     CDS             33614..35302
                     /gene="ilvB"
                     /locus_tag="ACHJZ2_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO4568177.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            35306..35596
                     /gene="ilvN"
                     /locus_tag="ACHJZ2_002064"
     CDS             35306..35596
                     /gene="ilvN"
                     /locus_tag="ACHJZ2_002064"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4568178.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            35859..>36377
                     /locus_tag="ACHJZ2_002065"
     CDS             35859..>36377
                     /locus_tag="ACHJZ2_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568179.1"
                     /translation="MASPINSGIMMANLCPSTFLDKNRNVAAELDIKNNEKKYSPGSN
                     FAKWMLQEIKRLILNIMSGSRSINTDILDYFHPMPGTENNGNRTWVAATGEDEYIEIK
                     QTGDKSFNITLVGRDKPSRKEIPYSGVAVATIIKSLSEKTSALETHSADTVLRKKLVN
                     SIVMKNTDFNYEI"
BASE COUNT         8989 a         9019 c         9470 g         8899 t
ORIGIN      
        1 gttgatgacc tgatggggaa atgacgtaaa aagacaaata actctataaa tgagcgaaat
       61 ttgatagttg atttttaaat agtaacctta ttattactac aactttcata tggatgaggg
      121 gaaagaaaat ggttgataac gtaactgtta gcagagtatg tatacaatct ccttctttcg
      181 tgcctgattt ggatggagaa aaaaataaat ctcaattatt cgttgacgat atagttgcgt
      241 atcttaaaag tccttcagtt tattcacttg aaaaagaggg gccgttaaat cattttgtta
      301 atcattgttc tgaagttgag ttaggtttct atagcgatgg tgcatattca attcttgtct
      361 ccagatctaa gcaacaacct gaaggtatga ttttaaccgt aagcgatgca gacgcaatca
      421 atatagtaca tatttccgta tctccagtgc ttataaaatt cctggatgat atttttactt
      481 gccttcatac gtaccctgat gatgagagtt ttacaaaaga gcagataaaa gctaatagca
      541 aatatgatat tgtagattat aattgcctgt tgcattttac tggaaaacca aaaagtttaa
      601 tagaatgtag acattttgct ctgcaatact gtatagattc aatgaatgag catacaggga
      661 aagttccatt aaaggcttac tattcatctc cggaagatat acaaaaacat attcctttcg
      721 agcttgagca gcaatttaac aatctacaaa aaaatccacc acccggtaca tgcgtcgttg
      781 ccagtgataa gtttggggag gcattatctg tcttttttca cagaatggaa aaagagaagt
      841 taacgcatat gacggcaatc gttcaatctc aaacacacgc tatggccgtc cgcttgagga
      901 tcaaaaaaac gcctgctggt gaaacagaat atgttgtatc cttttatgat cctaatgcaa
      961 ccaatactgc agtacgctat aaagcaaaca actgtgattc ttttgggtca ttgcaatcgt
     1021 ttataaatat tcaacaggca aaacaaaaat gggtaataac agatatttgc tccgagtgtg
     1081 taggaataac cccttatctc cctcgggaac aagcccattt attaagtggt attgaaaatg
     1141 agttgcaacc tccattatca ccaccagcat tatttctatt aatgagaatg gggatatata
     1201 aaaacattgt tctttttttc gataaattaa aaaactctca agaaatgaca gcatcaaagg
     1261 ctcttgatat tcttgctgcg aaatcacctg aaggaatata tgggttatgt gtattattgt
     1321 atcacaatac tattgataag tttaatgatt acataacaaa tttaaaagag ttgaccagaa
     1381 aatataattt tagccaagag gacctggaaa ctctactcct tgcgaaagat aatctcggag
     1441 tgagctggat tcccagggct ttgaaaaata atcaaaataa aattgtcaaa gcatggttgt
     1501 tggcgataga tgactttgag aaagaatttg gggtaaataa aaatgaaata cttcttcgta
     1561 taggaaagga aatagactca attgatgatt taaatagcgc tattagaacc aatgattata
     1621 atgttgttaa tatattgcta gccaatataa aagccaaaat gtttaaaaat gaattaaata
     1681 aagaagatat attgaaactg atggcagcaa gagaaaaagt ggcgggagca tcagacaaat
     1741 ggacgaaggc atcaggctta tattctgcga tagtgaaagg gcatacgaag attgttgctg
     1801 cctggatgga gacagctgaa gtgatagcca gccattatga aaatgataaa gatgtagtga
     1861 gagaactcct gtcgctgagc agaaataatg cagtttgctc tttgtatgtt gccagctata
     1921 agacaatgag taagcaggtc attgatgtat atctgaatgc ggcgattcgc ctggcgttgc
     1981 aacacgggtt cactttcgat gagattttgg agcagtttac ccgtgacttt gatgggaagt
     2041 cattctctct tgcggtagag aaagcggatg atatatatgg gtctctggct gaaaatattc
     2101 aaaattgtgg ttggtgaaaa tgaaaattat cttaaagatg tgatgatgca actggaagcg
     2161 aagaataatg aaggcaaata tgtaataagc aaggcaaatg gcaatcctgt atttaaagaa
     2221 ctattctgga aagcaataga cgaatttaac tttccgcaag aggagcttaa tcggctaaag
     2281 caatatcgtt ctctgtaaag acaggactgc aagatgttga aagtcgctcc agccagtagt
     2341 aaaggcagtt ttggtgcagg atgctcataa aaaaacaagc gctgttccgt tctgatagtt
     2401 agtttccccc agaacggaac agtgcccttt atatcaattt agacgactta cttaccaata
     2461 cagaagctgg agaaaatccg ccccagcaaa tcatcagagg taaattcccc ggtgatttcg
     2521 cttaagttct gctgtgccag gcgcaactct tccgccagca gttcgcccgc ccaggcaccc
     2581 aacagttgcg ctttaccttg ctgcaaatgc tccgctgcct gttccagcgc ctgaaggtgg
     2641 cgacgacgcg ccaggaagcc gccttccatg ttggtgtcaa agcccatgct ctgtttgaga
     2701 tggttacgca gcacgtccac gccttcgcca gtctttgccg agagacgaat caacgcatga
     2761 ccgttcactt cactcattcc cagcgtttcg ccagtgatat cggctttatt gcgtaccacg
     2821 gtgatcggca gtttcgcggg cagacgggcg ataaattccg gccagatctc cgccggatca
     2881 acggcgtctg tcgtggtgcc atcgaccata aacagcacgc ggtcggcttg ttcaatttct
     2941 tgccacgcgc gctcgatacc aatacgttcc acttcatcgc tggcttcacg tagcccggcg
     3001 gtgtcgatga tatgcagcgg cattccgtca atgtggatat gctcacgcaa cacgtcacgc
     3061 gtggttccgg cgatatcggt tacgattgct gcttcacggc cagccagcgc gtttaacagg
     3121 ctcgatttac cggcattagg acgtccggca atcaccacct tcatcccttc gcgcaacaaa
     3181 ctaccctgac gtgcttcagc acgcactgca tcgagatcgg caatcacgtc attaagctgg
     3241 gcttcaattt ttccgtcgga gaggaaatcg atctcttcat cggggaaatc aattgccgct
     3301 tcgacgtaaa tgcgcaagtg ggtgagggct tccaccagat ggttgacccg tgcggagaat
     3361 gcgccttgca gcgagttaag tgccgaacgg gctgcctgtt ccgaactggc atcgataaga
     3421 tcggcaatcg cctcggcctg ggctaagtcg agtttatcgt taagaaacgc gcgttcggaa
     3481 aactcaccag ggcgagcaat ccgcaggccg ggaatggtca gaatgcgttt taacagcagg
     3541 tcgagaatca ccggaccgcc atgaccctgc agttccagca catcttcgcc ggtgaacgag
     3601 ttcgggccag ggaaccatag cgcaatcccc tgatcgagca cgctgccatc ggcgtcttta
     3661 aacggaagat aatcggcgta gcgcggctta ggcagtttac ccagcacggt ttcggcaact
     3721 tcacgggctt tgaagccgga gatgcgcagg atgccaacgc cgccacgtcc cggaggcgtg
     3781 gcctgggcta cgatagtgtc attatcgctc atgatgttcc tgttgctttg tgtggcggat
     3841 acgcggtgct tatccgccct acgaaaagaa aaaaggcggt caactgaccg cccttatttt
     3901 agcgaaaact caccgaatca ggattttttc ttctcgcggc tatgcaggcc acgtttttcc
     3961 agaccacggt aaatcagctg ctgctgaata atggttacca ggttgctgac gatatagtac
     4021 agcaccagac ctgacgggaa ccacaggaag aacacggtga agatgaccgg cataaaggtc
     4081 atgatcttct gctgcatcgg gtcggtcaca gtggtcggcg acatcttctg aatgaagaac
     4141 atcgttacgc ccatcaggat cggcaggatg tagtacgggt cctgtgcaga caggtcgtgg
     4201 atccacagtg caaacggtgc ctgacgcagt tcaacggaac ccatcagcat gtagtacaac
     4261 gccaggaaga ttggcatctg gatcagcagc gggaagcagc cgcccagcgg gttaaccttc
     4321 tcagctttgt acagcgccat catttcctgg ctgatacgct gtttgtcatc gcccagacgc
     4381 tcacgcattg cctgaatctt cggctgcagc atacgcatct tcgccatgga ggtgtactgc
     4441 gctttggtca gcgggtacat gatgccacga acgataaagg tgatgatgat aatggagaag
     4501 ccccagttac ccacaaagct atggatccat ttcagcagtt tgaacagcgg ctgagagatg
     4561 aaccacaacc aaccgtaatc aacggtcaga tccaggtgcg gagcaacagc tgccattttg
     4621 tcctggattt ccgggccaac ccacagggtg ctgttcatcg cgccagtctg accaggctga
     4681 accagtaccg gctgagattt atagccgata gcggcgatgc cgttacccag attagcagta
     4741 tagaagttgt tggtaccgtc gttatgcggg atccacgccg tcgcgaaata ctgttgcagc
     4801 attgccaccc aaccaccttt cgaagagatg ttcaggtttt cgttatcggc aatggtatcg
     4861 aacttgtatt tctcgtactt ctcgtcaggc gtggagtacg ccgcgccacg gaaggtgtgc
     4921 agtgcgaagt tgctgcttcc ggtatcgaga tgcggtggca gagtgatgga ttgcttcaac
     4981 tgaccaaagg tggagatttc cagcggtttc tcgccagcgt tctgcacgtt gtagttgacg
     5041 ttgacagcgt aatcaccccg tttcaggaca aacgttttgg taaacgtgtt gcctgccgcg
     5101 tcggtatacg tcatcggcac ctgcagttcg ttttgacctt cagccagcac ataagcgtct
     5161 ttttcaacgt tatacagcgg acgcgggccg ttagccgggt tatctgggcc atcacgaccg
     5221 gtcagaccgc tctgtgcctg ataaataaac tgcggtgaag tttccagcag ctggaacggc
     5281 tgggtagagt tcagctcttt cgggtaagca ggcagcagag cttgctcaac atcaccacca
     5341 cgggtgttga tggtcagatc aagcacgtcg gtcttaaccg agatcagttt cccctggcca
     5401 ctggccggta cgccctggtc ggcggcgcta cccgctgcgg tggtcgttgt ctgcgtggtc
     5461 tgttgggcct gaggttgcgg gtttttatcc tgctcccagg cttgccagat catgaaagac
     5521 acgaacagca aagcgatgac taaaagattg cgttgcgaat ccatcgttag tgttctctgg
     5581 tattaaatgg tccgggcggg acgggatcgt caccaccagg gtgtaaaggg tggcatttta
     5641 atacgcgttt caccgtcaac caactgcctt ttatcactcc aaacctgcgc aatgcctcaa
     5701 ttccgtagct tgaacaggtt ggagtgaaac gacaatgcgg cccgagtagc ggactaatca
     5761 ggcgttgata gacccgaatg agggctatca ggacccgcga gccaggcgac agtggcggcg
     5821 ccataatttt tccaacgctt ccgagagagc acggttatcg aggttggcaa cccctttttt
     5881 cgccaccacc acgaaatcca tagccgggag ttcatgttgg cgcagacgga agctttcacg
     5941 cgtcagacgt ttaatccgat tgcgttcatg ggcgcgtcga acgtttttct tggcgactgt
     6001 aagaccgata cggggatgcc ccagcgaatt caggcggccg agaatggtaa tttgcggcgt
     6061 gccagcccgt tgtggctgct ggaagacgaa tgtgaattga ctgggagtta acaagcgtaa
     6121 ctccctggga aatgcgagct taaccactca ggggttagct ttattactta gaaacggtca
     6181 gacgagcgcg gcctttagca cgacgacgtg ccagaacctg acgaccattt ttagtagcca
     6241 tacgagcacg gaagccgtga gaacggttgc gcttcagtac agacggttga aaagtgcgtt
     6301 tcatggcgat ttctacctaa acttgaataa attcaatggc tttattggat atccgccgaa
     6361 aaatgaaacg atggacaccg aagccatggg tgattaaaga ggccggattg taataattgt
     6421 acactccgga gtcaattctc tttccttatt taccgcgctt ttccgcacct tttcgcaggg
     6481 aaaacgtacg gcctcacacc agtgaaaacc agcatggcgc gccgggtgga ggattatacg
     6541 ggctgatggg taaagcgcaa ggatcgtcct ggatctttat tagatcgatt aagccaattt
     6601 ttgtctatgg tcattaaatt ttccaatatg cggcgtaaat cgtgcccgcc tcgcggcagg
     6661 atcgtttaca cttagcgagt tctggatcat cctgtggata aatcgggaaa atctgtgaga
     6721 aacagaagat ctcttgcgca gtttaggcta tgatccgcgg tcccgatcgt tttgcaggat
     6781 cttgatcggg catataaccg cagacagcgg ttcgtgcgtc accctcaagc agggtctttt
     6841 cgacgtacgt caacaatcat gaatgtttca gccttagtca ttatcgactt ttgttcgagt
     6901 ggagtccgcc gtgtcacttt cgctttggca gcagtgtctt gcccgattgc aggatgagtt
     6961 accagccaca gaattcagta tgtggatacg cccattgcag gcggaactga gcgataacac
     7021 gctggccctg tacgcgccaa accgttttgt cctcgattgg gtacgggaca agtaccttaa
     7081 taatatcaat ggactgctaa ccagtttctg cggagcggat gccccacagc tgcgttttga
     7141 agtcggcacc aaatcggtga cgcaaacgcc acaagcggca gtgacgagca acgtcgcggc
     7201 ccctgcacag gtggcgcaaa cgcagccgca acgtgctgcg ccttctacgc gctcaggttg
     7261 ggataacgtc ccggccccgg cagaaccgac ctatcgttct aacgtaaacg tcaaacacac
     7321 gtttgataac ttcgttgaag gtaaatctaa ccaactggcg cgcgcggcgg ctcgccaggt
     7381 ggcagataac cctggcggtg cctataaccc gttgttcctt tatggcggca cgggtctggg
     7441 taaaactcac ctgctgcatg cggtgggtaa cggcattatg gcgcgcaagc cgaatgccaa
     7501 agtggtttat atgcactccg agcgctttgt tcaggacatg gttaaagccc tgcaaaacaa
     7561 cgcgatcgaa gagtttaaac gctactaccg ttccgtagat gcactgctga tcgacgatat
     7621 tcagtttttt gctaataaag aacgatctca ggaagagttt ttccacacct tcaacgccct
     7681 gctggaaggt aatcaacaga tcattctcac ctcggatcgc tatccgaaag agatcaacgg
     7741 cgttgaggat cgtttgaaat cccgcttcgg ctgggggctg actgtggcga tcgaaccgcc
     7801 agagttagaa acccgcgtgg cgatcctgat gaaaaaggcc gacgaaaatg acattcgttt
     7861 gccaggcgaa gtggcgttct ttatcgccaa gcgtctacga tctaacgtac gtgagctgga
     7921 aggcgcgctg aaccgcgtca ttgccaacgc caactttacc ggacgggcga tcactatcga
     7981 cttcgtgcgt gaggcgctgc gcgacttgct ggcattgcag gaaaaactgg tcaccatcga
     8041 caatattcag aagacggtgg cggagtacta caagatcaaa gtcgcggatc tcctttccaa
     8101 gcgtcgatcc cgctcggtgg ctcgtccgcg tcagatggcg atggcgctgg cgaaagagct
     8161 gactaaccac agtctgccag agattggcga tgcgtttggt ggtcgtgacc acactacggt
     8221 gcttcatgcc tgccggaaga tcgagcagct gcgtgaagag agccacgata tcaaagaaga
     8281 tttttcaaat ttaatcagaa cattgtcatc gtaaacctat gaaatttacc gtagaacgtg
     8341 agcatttatt aaaaccgcta caacaggtga gcggtccgtt aggtggtcgt cctacgctac
     8401 cgattctcgg taatctgctg ttacaggttg ctgacggtac gttgtcgctg accggtactg
     8461 atctcgagat ggaaatggtg gcacgcgttg cgctggttca gccacacgag ccaggagcga
     8521 cgaccgttcc ggcgcgcaaa ttctttgata tctgccgtgg tctgcctgaa ggcgcggaaa
     8581 ttgccgtgca gctggaaggt gagcggatgc tggtgcgctc cgggcgtagc cgtttttcgc
     8641 tgtctaccct gccagcggcg gatttcccga acctcgatga ctggcagagt gaagtcgaat
     8701 ttaccctgcc gcaggcgacg atgaagcgtc tgattgaagc gacccagttt tctatggcgc
     8761 atcaggacgt tcgctattac ttaaacggta tgctgtttga aaccgaaggt gaagaactgc
     8821 gcaccgtagc gaccgacggc caccgtctgg cggtctgttc aatgccaatt ggtcaatctt
     8881 tgccaagcca ttcggtgatc gtgccgcgta aaggcgtgat tgaactgatg cgtatgctcg
     8941 acggcggcga taatccgctg cgtgtgcaga ttggcagcaa caatattcgc gcccacgttg
     9001 gcgactttat cttcacctct aaactggtgg atggtcgctt cccggattac cgccgcgttc
     9061 tgccgaagaa tccggacaaa catctggaag caggctgcga tctcctcaag caggcgtttg
     9121 ctcgcgcggc aattctctct aacgagaaat tccgcggtgt gcgcctgtat gtcagcgaaa
     9181 accagctgaa aatcaccgcc aacaacccgg aacaggaaga agcggaagag atcctcgacg
     9241 ttacctatag cggtgcggag atggaaatcg gcttcaacgt cagctatgtg ctggatgttc
     9301 tgaacgcgct gaaatgcgaa aacgtccgca tgatgctgac cgattcggtt tccagcgtgc
     9361 agattgaaga tgcagccagc cagagcgcgg cttatgttgt catgccaatg agactgtaat
     9421 gtccctcacc cgcttgttga tccgcgattt ccgcaacatt gaaaccgcgg atctcgcttt
     9481 atctcccggc tttaactttc tggtaggtgc caacggcagt ggcaaaacca gcgtgctgga
     9541 agccatctat acgctcggcc atggtcgggc gtttcgcagt ttgcagattg gtcgcgtcat
     9601 tcgccacgag caggaggcgt ttgttctcca cgggcgatta cagggcgaag agcgcgagac
     9661 ggcgattggc ttaaccaaag acaaacaggg cgacagcaaa gtccgcatcg acggtactga
     9721 cgggcataaa gtcgcggaac tggcgcacct gatgccaatg cagctgataa cgccagaagg
     9781 gtttacttta ctcaacggcg gccccaaata cagaagagca ttcctcgact ggggatgctt
     9841 tcacaacgaa cccggatttt tcaccgcctg gagcaatctc aagcgattgc tcaagcagcg
     9901 caatgcggcg ctgcgccagg tgacacgtta cgaacagcta cgcccgtggg ataaagaact
     9961 gatcccgctg gcggagcaaa tcagtacctg gcgcgcggag tatagcgccg gtatcgcggc
    10021 cgatatggct gatacctgta agcaatttct ccctgagttt tctctgactt tctctttcca
    10081 gcgcggctgg gagaaagaga cagaatatgc tgaggtgctg gaacgtaatt ttgaacgcga
    10141 tcgccagcta acctacaccg cgcatggccc gcataaagcg gacttacgca ttcgcgccga
    10201 cggtgcgccg gtggaagata ccttatcgcg tgggcagctt aagctgttga tgtgcgcctt
    10261 acgtctggcg caaggagagt tcctcacccg tgaaagcggg cggcggtgtc tctacctgat
    10321 agatgatttt gcctctgagc ttgatgatga gcgtcgcggg ctgcttgcca gccgcttaaa
    10381 agctacgcaa tcgcaggtct ttgtcagcgc gatcagtgct gaacacgtta tagacatgtc
    10441 ggacgaaaat tcgaagatgt ttaccgtgga aaagggtaaa ataacggatt aacccaagta
    10501 taaatgagcg agaaacgttg atgtcgaatt cttatgactc ctccagtatc aaagtcctga
    10561 aagggctgga tgcggtgcgt aagcgcccgg gtatgtatat cggcgacacg gatgacggca
    10621 ccggtctgca ccacatggta ttcgaggtgg tagataacgc tatcgacgaa gcgctcgcgg
    10681 gtcactgtaa agaaattatc gtcaccattc acgccgataa ctctgtctct gtacaggatg
    10741 acgggcgcgg cattccgacc ggtattcacc cggaagaggg cgtatcggcg gcggaagtga
    10801 tcatgaccgt tctgcacgca ggcggtaaat ttgacgataa ctcctataaa gtgtccggcg
    10861 gtctgcacgg cgttggtgtt tcggtagtaa acgccctgtc gcaaaaactg gagctggtta
    10921 ttcagcgcga gggtaaaatt caccgtcaga tctacgaaca cggtgtaccg caggcaccgc
    10981 tggcggttac cggcgagact gaaaaaaccg gcaccatggt gcgtttctgg cccagcctcg
    11041 aaaccttcac caatgtgacc gagttcgaat atgaaattct ggcgaaacgt ctgcgtgagt
    11101 tgtcgttcct caactccggc gtttccattc gtctgcgcga caagcgtgac ggcaaagaag
    11161 accacttcca ctatgaaggc ggcatcaaag cgttcgttga atatctgaac aagaacaaaa
    11221 cgccgatcca cccaaatatc ttctacttct ccaccgaaaa agacggtatc ggcgttgaag
    11281 tggcgctgca gtggaacgat ggcttccagg aaaacatcta ctgctttacc aacaacattc
    11341 cgcagcgtga cggcggtact cacctggcag gcttccgtgc ggcgatgacc cgtaccctga
    11401 atgcctacat ggacaaagaa ggctacagca aaaaagccaa agtcagcgcc accggtgacg
    11461 atgcgcgtga aggcctgatt gcggtcgttt ccgtaaaagt gccggacccg aaattctcct
    11521 cccagaccaa agacaaactg gtttcttctg aggtgaaatc ggcggttgaa cagcagatga
    11581 acgaactgtt ggcggaatac ctgctggaaa acccaactga cgcgaaaatc gtggttggca
    11641 aaattatcga tgctgcccgt gcccgtgaag cggcgcgtcg cgcgcgtgaa atgacccgcc
    11701 gtaaaggtgc gctcgactta gcgggcctgc cgggcaaact ggcagactgc caggaacgcg
    11761 atccggcgct ttccgaactg taccttgtgg aaggggactc cgcgggcggc tctgcgaagc
    11821 aggggcgtaa ccgcaagaac caggcgattc tgccgctgaa gggtaaaatc ctcaacgtcg
    11881 agaaagcgcg cttcgataag atgctctctt ctcaggaagt ggcgacgctt atcaccgcgc
    11941 ttggttgtgg tatcggtcgt gacgagtaca acccggacaa actgcgttat cacagcatca
    12001 tcatcatgac cgatgcggac gtcgacggct cgcacattcg tacgctgctg ttgaccttct
    12061 tctatcgtca gatgccggaa attgttgaac gcggtcacgt ctacatcgct cagccgccgc
    12121 tgtacaaagt gaagaaaggc aagcaggaac agtacattaa agacgacgaa gcgatggatc
    12181 agtaccagat ctctatcgcg ctggacggcg caacgctgca caccaacgcc agcgcaccgg
    12241 cattggctgg cgaagcgtta gagaaactgg tgtctgagta caacgcgacg cagaaaatga
    12301 tcaaccgcat ggagcgtcgt tatccgaaag caatgctgaa agagcttatc tatcagccga
    12361 cgctgacgga agccgacctc tctgatgagc agaccgttac ccggtgggtg aacgcgctgg
    12421 tcagcgaact gaacgacaaa gaacagcacg gcagccagtg gaagtttgat gtccacacca
    12481 atgccgaaca aaacctgttc gagccgattg ttcgcgtgcg tacccacggt gtggatactg
    12541 actatccgct ggatcacgag tttatcactg gtggcgaata tcgtcgtatc tgcacgctgg
    12601 gtgagaaact gcgtggcttg ctggaagaag atgcgtttat cgaacgtggc gagcgtcgtc
    12661 agccggtagc cagcttcgag caggcgctgg actggctggt gaaagagtcc cgtcgcggcc
    12721 tctccatcca gcgttataaa ggtctgggcg agatgaaccc ggaacagctg tgggaaacca
    12781 ccatggaccc ggaaagccgt cgtatgctgc gcgttaccgt taaggatgcg attgccgctg
    12841 accagttgtt caccacgctg atgggcgacg ccgttgaacc gcgccgtgcg tttattgaag
    12901 agaacgccct gaaagcggcg aatattgata tttaatggcg ttaacaatgc gagcgtgcct
    12961 gatgcgctac gcttatcagg cctacgagac tcctgcaata tattgaattt gcatgttttt
    13021 gtaggccgga taaggcgttc acgccgcatc cggcaagaag ctataagaaa agggcggaga
    13081 tcatctccgc cctttttatt tctgcaatcc gcaattatca tcccgcagtt ctatactttt
    13141 tgggtcaaca cgtaacgctt tacaggtgat tatcatgggg ctttttgatg aagttgtcgg
    13201 cgcctttctg aaaggcgatg cggggaaata tcaggcgatt ttaagttggg ttgaggagca
    13261 gggtggcatt caggtgctgc tggaaaaact gcaaagtggc ggcttagggg ccattctctc
    13321 aacctggctg agtaatcaac agggcaatca accagttagc ggcgagcaac tggaatcagc
    13381 actcggcaca aacgcggtat ccgatcttgg acaaaaactt ggcgtggata ccagtacggc
    13441 ttcctgttta ctggcagaac aattgccgaa gattattgat gcgctctcac cgcaaggtga
    13501 agtgtcacca caagccaata acgatctgct ttccgcaggc atggaactgc tgaaagggaa
    13561 actcttccgc taagcaaatg gggagcatgc aacccgcgtt gcatccccct tttccctcgg
    13621 gtgttgtact gatttttgag cggaatcgcg ttagcatggg tcaggaacca atctacctgg
    13681 ggaactcatg gctatcaaac tcattgctat cgatatggat ggcacccttc tgctgcccga
    13741 tcacaccatt tcacctgccg ttaaaaatgc gattgccgca gctcgcgccc gtggcgtgaa
    13801 tgtcgtgcta acgacgggtc gcccttatgc aggtgttcac aattacctga aagagctgca
    13861 tatggaacag ccgggcgact actgcattac ttataacggc gcgctggtac agaaggccgc
    13921 tgatggtaac accgtggcgc aaactgctct cagctatgac gactatcgtt tcctggaaaa
    13981 actctctcgc gaagtcggtt ctcatttcca cgccctggac cgcaccacgc tgtacaccgc
    14041 caaccgtgat atcagctact acacggtgca tgaatccttc gttgccacca ttccgctggt
    14101 gttctgcgaa gcggagaaaa tggaccccaa tacccagttc ctgaaagtga tgatgattga
    14161 tgaacccgcc atcctcgacc aggctatcgc gcgtattccg caggaagtga aagagaaata
    14221 taccgtgctg aaaagtgcgc cgtacttcct cgaaatcctt gataaacgcg ttaacaaagg
    14281 tacgggtgtg aaatcactgg ccgacgtgtt aggtattaaa ccggaagaaa tcatggcgat
    14341 tggcgatcag gaaaacgaca tcgcaatgat tgaatatgca ggcgtcggtg tggcaatgga
    14401 taatgctatt ccttcggtga aagaagtggc gaactttgtc accaaatcca accttgaaga
    14461 tggcgtggcg tttgctattg agaagtatgt gctgaattaa tctgtgggcg ggcaaacagc
    14521 ataaacgcgt ttgcccgctt actgattatt ggtttactgc atatacaacc agaaattgca
    14581 gcggcgcagt aatgatgtcc agcgtaacag tcactggcag taatgctgcg gaaacaccat
    14641 ccggaatgcc tctgggtgaa tagtattcat aaaatcgcac tttaaatgga tggtagaacg
    14701 ccatcacctt tgagtaatcc atgtttttat ttttctcatg gattgtgcct tttaggtttt
    14761 ccagtgacct ggtacagtgt tgaacaccac aggcaaaacc ataatgcgtt gccagtgcct
    14821 tttcttcttc gttattccag tagtagtaaa gcgatattgt gccggtaaat ttctttttcc
    14881 ccgcatttaa aacaaatctt gcgtcattgg caacctgaat attgtgccgg ttaagtgctg
    14941 gatcattgag catcttaacg atgtcatcgg caccatcagt gataaggtaa tccagcgatt
    15001 cccctacaaa aacatagcct tttgttccat tactatcttt tgcctgcgat agcccagtga
    15061 tggtgtctga ctgcgattga tactttcctg aactaccctt tgcggtatgg gaaaccagag
    15121 catacgagat acagcctgaa agcatcagcg ccagtgggaa taaactggca gccttaaaaa
    15181 atcccttaaa attcaacttc atgattaatc ctttaaacga gtagctaagc cgttgaaagc
    15241 atactcttct ttcccccctg tttagcgcct gatctccctt ttcagatttc tgcccgatat
    15301 aagacctttt cttatgaggt gtttttttgt ggactaatat ctgttgcaag gctatgggtt
    15361 cgcacctgca ctggtttata tctccgccgt cgcgttgatt ggcgcgctct cttacatcct
    15421 gctggtgggc gatgtgacgc gcgttggcta atctttcaac tgtggaatat gcgccatacc
    15481 actgcgcata atccactttc ttaaagccgg tgcggtcagg catcggcttt ttactatctt
    15541 gccgaagagt tttaaacgca gtttgcgggt ggcgtgccag taggctgcgt tgatcttctc
    15601 tggctgcttc agtaacaccg aacgcagaat ctctgcgcta tccagcgcat agcttatccc
    15661 ctccagcgag ctggcgctga taaatcccgc cgcttcacca atcagaaagg cgttgtcctt
    15721 accgcagaca aaatcctgcc agcgtgaggg aaatagcacc gtgcattttt cgctttttac
    15781 cgccttacca aactggaatt gaaaggcgct cattttctct ttcagcgtcg tgaaacgcgt
    15841 ctgaccgtct ttcattggat aggcaccgcc aaagataaaa taaccgtctt tgctgatact
    15901 ccatgagtaa cagtcagtta tcgcattatc aaagatgcat gagtagaacg gcaccggatg
    15961 tttctctgcg aaccactgct ggatagcgac atatttacga atctgatggt ccgggtagag
    16021 atatcgccgc accatcgagt tggcaccatc tgcaccgacc agatagcggg cagtaatatg
    16081 ctgctcccaa ccgtctgcac gaaaaatgac atgccattta tcatcctcac gccagatttt
    16141 ccggcacagg ctatcgtgat aaacctcaac gctggcgggg atcagcgatt tcatccacaa
    16201 gtcgaaagca tggcgattaa tattgatata gcttcgctgg tagttacgcg tgagcgatgc
    16261 ggcgacgtcg acagttttga cgctgaaaat ctgtggattg gcgatcacat cgacaggaag
    16321 cgtcagtcca tcgcgaataa aagatcgctg cgcgtccggt gccagcagac cgccacaagg
    16381 cttgctgaaa ccttcagtac catgctggtg ctttttatcc agcgcgatca cctgcatttt
    16441 gccggctaac tttcgtgcca acgccgaccc tgccgggccg aggccaataa tcgccacgtc
    16501 gaaatgttcc atcttttcac tcacattcat cacgatggca aaagactatt gtctgaaagt
    16561 gaagtccttg tgaagtatga tggcgggaac cgttttgtat caggaccgcg ccatgaaaca
    16621 gataaccttt gctccccgta atcacctgct caccaatacc aatacctgga cgcccgacag
    16681 ccagtggctg gtatttgacg tgcgtccttc tggcgcgtcg tttaccggcg agaccattga
    16741 gcgtgtgaat atccataccg gcgaggtcga ggttatctat cgcgtgtcac agggcgcgca
    16801 cgtcggcgtg gtgaccgttc atccaaagtc agagaaatat gtctttatcc acggacctga
    16861 aaatcctcat gaaacatggc attacgattt ccatcaccgt cgcggcgtga ttgttgaagg
    16921 cggcaagatg aacaatctcg atgcaatgga tattaccgtt ccgtacacat caggggcgct
    16981 gcgtggcggc agccatgtgc atgtctttag cccgaacggt gaaatggtga gttttaccta
    17041 taacgaccat gtaatgcatc agttcgattc ggcgctggat ttgcgaaacg tcggggttgc
    17101 tgcaccgttt ggcccggtca acgtacaaaa gcagcatccg cgtgaataca gcggtagcca
    17161 ctggtgcgtg ctggtgagta aaaccacgcc cacgccgcag cctggcagcg atgaaatcaa
    17221 tcgtgcttat gaagaaggat gggtaggaaa tcacgcgctg gcgtttattg gcgacacact
    17281 ttcgccaaag ggcgagaaag tgccggagct gtttatcgtt gagttaccgc aagatgaagc
    17341 tggctggaaa gcagcaggtg atgcgccgtt aagtggaacg gaaacaaccc tgcccgcgcc
    17401 accgcgtggc atcgtgcagc gacgtttaac ctttacccac catcgggctt atccggggtt
    17461 agtcaacgtc ccgcgccact gggtgcgctg taatccgcag ggtacgcaaa tcgcgttttt
    17521 aatgcgagat gataacggca ttgtgcaact gtggcttatc tcgccacagg gcggcgagcc
    17581 gcgccagtta acccataaca aaacggatat tcagtctgca tttaactggc atccgtcagg
    17641 agaatggttg ggctttgtgc tgggtaatcg aattgcttgt gcccatgcgc aaagcggcga
    17701 ggttgagtat ttaaccgaaa accacgccaa tccaccttct gcggacgccg tggtcttctc
    17761 gccggatggt cagtggctgg cgtggatgga aggcggccag ctgtggatca ccgaaactga
    17821 tcgctaaatc agttatcagg cattctggcc ggtggaataa ccgcattggt ggcttgcgcg
    17881 ttggcttcgc ttttttcaac gcgtgagcgc accgagctgt ctttgcggaa cacatcccac
    17941 ggtaataaaa gcgtgtccat cacagcggta aacggcatat cgagaatcgc cagcgactta
    18001 gtgccccagt tggtttcatc atcgccaatc atggtggcgc tggcgcgggt gccgggatac
    18061 gttccttctt tccctccggt atgggacatc acgctggagc atcctccgag taatgccatt
    18121 ccgctgcata tcatgaacgc taacaacaca tttcttatca taataaattc atctgttgat
    18181 cgtgggtgtt ggcctgatga gttatagcga tcccttgctg aaaataacat catcattacg
    18241 tcgctctgtg gcggctatcg cactttaacg tttcgtgctg ccccctcagt ctatgcaata
    18301 gaccataaac tgcaaaaaaa agtccgctga taaggcttga aaagttcatt tccagaccca
    18361 tttttacatc gtagccgatg aggacgcgcc tgatgggtgt tctggctacc tgacctgtcc
    18421 attgtggaag gtcttacatt ctcgctgatt tcaggagcta ttgattatgc gtaactttga
    18481 tttatccccg ctttaccgtt ctgctattgg atttgaccgt ttgtttaacc acttagaaaa
    18541 caaccagagc cagagtaatg gcggctaccc tccgtataac gttgaactgg tagacgaaaa
    18601 ccattaccgc attgctatcg ctgtggctgg ttttgctgag agcgaactgg aaattaccgc
    18661 ccaggataat ctgctggtgg tgaaaggtgc tcacgccgac gaacaaaaag agcgcaccta
    18721 tctgtaccag ggcatcgctg aacgcaactt tgaacgcaaa ttccagttag ctgagaacat
    18781 tcatgttcgt ggtgctaacc tagtaaatgg tttgctgtat atcgatctcg aacgcgtgat
    18841 tccggaagcg aaaaaaccgc gccgtatcga aatcaactaa tttcctaagg ccgccttgcg
    18901 cggcctgaca tctccatgct cgccgtcagg gagcatatgc gaatcttcgg atttgcaggt
    18961 acttactcgc ttcttagaag gagaaatgac tatgcgtaac ttcgatttat ccccactgat
    19021 gcgtcaatgg atcggttttg acaaactggc caacgcactg caaaacgccg gtgaaagtca
    19081 gagcttcccg ccgtacaaca ttgagaaaag cgacgataac cactaccgca ttacccttgc
    19141 gctggcaggt ttccgtcagg aagatttaga gattcaactg gaaggtacgc gcctgagcgt
    19201 aaaaggcacg ccggagcagc caaaagaaga gaaaaaatgg ctgcatcaag ggcttatgaa
    19261 tcagccattt agcctgagct ttacgctggc tgaaaatatg gaagtctctg gcgcaacctt
    19321 cgtaaacggt ttactgcata ttgatttaat tcgtaatgag cctgaaccca tcgcagcgca
    19381 gcgtatcgct atcagcgaac gtcccgcgtt aaatagctaa ctagctattc tctttgcccc
    19441 gccattctgg tggggctttt tttgtctttc ttcttcaaca atgcgttaaa tttcgactgt
    19501 ttaagatatt tcggcacgtt ctttcccgtg ataatgtgcg ctataaggca aatctgcttc
    19561 accgattcag ccataatccg tagcaattaa aatgttatct caagagaaat ggacgatgag
    19621 tgatatagca ttaacggtca gtattttggc tttggtggca gtcgtcggtt tgtttatcgg
    19681 caacgtcaaa tttcgcggca taggattagg tattggcggc gtgctgtttg gtgggatcat
    19741 cgtcggccat tttgtttctc aggcggggat gacattaagt agcgatatgc tgcatgttat
    19801 tcaggaattt ggcctgatcc tgttcgttta taccatcggg attcaggtag ggccgggctt
    19861 ctttgcctca ttgcgcgtct ccggattacg cctcaacctg tttgctgttc tgatcgtcat
    19921 catcggtggt ctggttaccg ccatcctgca taaactgttt gatattccac tgccggtagt
    19981 gctggggatt ttctccggtg cggtaaccaa tacgccagcg ctgggggcag ggcagcagat
    20041 tttgcgcgac ctgggtacac caatggaaat ggtcgatcag atggggatga gttacgcgat
    20101 ggcgtatcca ttcggcattt gcgggatatt gttcaccatg tggatgttgc gggttatttt
    20161 ccgcgtcaat gtcgagacag aagcccagca gcacgagtct tcacgcacca atggcggcgc
    20221 gctgatcaag actatcaata ttcgcgttga gaaccctaac ctgcatgatt tagccattaa
    20281 agatgtaccg attctcaacg gcgacaaaat tatctgctcg cgtctgaaac gcgaagaaac
    20341 cctaaaagtt ccttcgccag ataccattat ccaactgggc gatttgctgc atctggtggg
    20401 tcagccagcg gatttacata atgcgcaact ggtgattggt caggaggtcg atacttcgct
    20461 gtccacgaaa ggcactgatt tgcgcgtcga gcgtgtggtg gtcaccaatg aaaacgtgct
    20521 tggtaagcgt attcgcgacc tgcattttaa agaacgctat gacgttgtta tctcgcgcct
    20581 gaaccgtgcc ggggtcgaac tggtcgccag tggcgatatc agcctgcagt tcggcgatat
    20641 cctcaacctg gtggggcgtc cgtccgcaat tgatgccgtt gccaatgtgc tggggaatgc
    20701 gcagcaaaaa ctgcaacagg ttcagatgct gccagtgttt attggcatcg ggcttggcgt
    20761 attgttaggt tctattcccg tctttgtgcc aggattcccg gccgcgttga aactggggct
    20821 ggcgggcggt ccgctgatta tggcgttgat cctcgggcgt atcggcagta tcggcaagct
    20881 gtactggttt atgccgccaa gtgccaacct cgcgctgcgg gaactgggga tcgtgctgtt
    20941 cctctcggtc gttggtctga aatctggtgg ggattttgtg aataccctgg tcaatggcga
    21001 agggctaagc tggattggtt atggtgccct gatcaccgcc gttccgctga ttactgttgg
    21061 cattctggcg cggatgttag ccaaaatgaa ttacctgacc atgtgcggga tgctggcagg
    21121 ttccatgacc gatcctccgg cgctggcgtt tgctaataat cttcatccaa ccagcggtgc
    21181 ggcggcgctc tcttacgcca ctgtctatcc gttagtgatg ttcctgcgca ttatcacccc
    21241 ccaattactg gcggtgctct tctggagtat cggttaactc ttgtccgggt ggagtcgccg
    21301 taaatggtac tccacctggt attcgctggc atttctgaac atcactgaat aattcaaaaa
    21361 ctccccgctc tcgctatatg acaacgaggt gatccgcagc agtggcgtgt gttccgctac
    21421 cttcatttgc cgcgccagca gtctatcggc gagcaccggc gtcaggcttt cataattacc
    21481 gccaatcaaa atcccgcact cttgttcaat atattcaaac ttcgaccctt ccagatgttg
    21541 cagcgaaagg ttacggaaca gttttaccgg catatagctg tcttccagca tcagcggttt
    21601 cccttccaca aaacgaacgc gacgggagaa gtagatctgc tcgttgattt gaatccgcaa
    21661 ctggctggca attgccggag gcgcaggcat gatttcaaaa atcagcacct ggctggtgac
    21721 tgtttttccc tgccgtttta acacctccac cagcccggtc aggctggcgg tttgatgcag
    21781 cacatctttg cgcaccaggt aagtgccgct gccgtggcgg cggaccacca gcccccacgc
    21841 taccagcagg tcaatcgctt tacggatggt catccgcgat accgcaaact cttccgccag
    21901 ctttttttca ccgggaagaa ggctgtttag cgtgaaatcc gcggaattaa gtcgaattct
    21961 taaccgctcc gcaatgcttt tgtagatcat ctctagatct cttttttgcc tgcaagcccg
    22021 ggtaatgcgc ccacaagttc tgcttaatca attgaaaaaa catctttttt ttaaagatgt
    22081 gttcgatgct gtgtaccagt gctcacagag atctactttt tcgcgaaaaa gtagatctct
    22141 accgcccaac gaaaagcatg aaagcgatca cgaatcccat tgggtcgtca tgttctgccg
    22201 atcgcatctt cctatcctcg ctccaggcct gccgcataac caatcaggct tcctacttac
    22261 agaattgaga aaagaggatg tggaaatgct cagtcaaatt caacgctttg gcggcgcgat
    22321 gttcacgcca gtgctgctgt ttcccttcgc cgggattgtg gtgggtcttg ccatcttgct
    22381 gcaaaacccg atgtttgtcg gggaatcact gaccgatccg aacagtttat tcgcgcaaat
    22441 cgtacacatt attgaagagg gcggttggac ggtattccgt aatatgccgc tgatttttgc
    22501 tgtcggttta cccattggcc ttgctaagca agcgcagggg cgtgcttgtc tggcggtgat
    22561 ggtgagtttc ctgacctgga actatttcat caatgcgatg ggaatgacct ggggaagcta
    22621 cttcggcgtc gatttcactc aggacgcggt ggcaggtagc ggtctgacaa tgatggccgg
    22681 gattaaaacc ctcgatacca gcattatcgg cgcaattatc atttccggca ttgtgacggc
    22741 gctgcataac cgtctgttcg ataaaaaact gccggttttt ctcggcattt tccaggggac
    22801 gtcttatgtg gtgattatcg ccttcctggt gatgatcccc tgtgcctggc tgacattgct
    22861 cggctggcca aaagtacaaa tggggattga atctctgcaa gcgttcctgc gttcggcggg
    22921 tgcgcttggg gtgtgggttt acaccttcct cgaacgtatt ctgatcccaa ccggtttaca
    22981 ccacttcatc tacggaccgt ttatctttgg tccggcagct gttgaaggcg gaattcagat
    23041 gtactgggcg cagcatctgc aagagttcag tttgagcgcc gagccgctga aatcgttgtt
    23101 cccggaagga ggttttgccc tgcacggtaa ctcaaaaatc tttggtgccg tgggcatttc
    23161 tttagcgatg tacttcactg ccgcaccgga aaatcgggta aaagtggcgg gtttgctgat
    23221 tcccgcaacc ttaaccgcca tgctggtggg aattaccgaa ccgctggaat ttaccttcct
    23281 gttcatttca ccgttgctgt ttgcggtaca cgctgtgctt gcggcctcaa tgtcgaccgt
    23341 gatgtatctc tttggtgtgg tgggcaacat gggcggaggt ctgattgacc aggttttacc
    23401 gcaaaactgg atcccgatgt tcagcaacca cgcggatatg atgctgaccc aaatcgccat
    23461 tgggttgtgc tttaccctgc tgtacttcgt ggttttccgc accctgattc tgcaattcaa
    23521 catgtgcacg ccgggacgtg aagatgcgga agtgaaactc tactcaaaag ccgaatacaa
    23581 agcctcgcga ggccaaacca ccgcggcaga gccaaaaaaa gagctggatc aggctgccgg
    23641 tatcctgcaa gccctgggcg gggtcggcaa tatctccagc attaacaatt gtgcgacgcg
    23701 tctacgcatt gcactgcatg acatgtcaca aacgctggat gacgaagtct ttaaaaagct
    23761 gggagcgcac ggcgtcttcc gtagtggcga tgccattcag gtgatcattg gtctgcatgt
    23821 atcccagctg cgtgaacagc tcgatagctt aattaattct catcaatcag cagaaaatgt
    23881 tgccattacg gaggcagtat aatgaccaaa ttctcagtgg ttgtcgcagg cggtggaagc
    23941 acctttacgc caggcatcgt gttgatgctc ctggcgaatc aggaccgttt cccgcttcgt
    24001 gcactgaaat tttatgataa cgatggttcg cggcaggaag tgattgccga agcctgtaaa
    24061 gtcatcctta aagaaaaagc gccggatatt gcgtttagtt acaccaccga tcctgaagtg
    24121 gcgttcagcg acgttgattt tgtcatggcg cacatccgcg tcggcaaata cccgatgcgc
    24181 gaactggatg aaaaaatccc gctgcgccac ggcgttgttg gtcaggaaac ttgcggaccc
    24241 ggcggaatag cgtacggcat gcgttccatt ggcggcgtcc tggaactggt ggattatatg
    24301 gaaaaatatt caccaaacgc ctggatgctc aactactcca acccggcagc aattgttgca
    24361 gaagccacgc gtcgtctgcg cccgaatgcg aaaatcctca acatctgtga catgccaatc
    24421 ggtattgaaa gccggatggc gcaaattgtt gggctgcaag atcgcaaaca gatgcgcgtg
    24481 cgctactacg gcctgaacca ctttggctgg tggacatcaa ttgaagattt gcagggcaac
    24541 gacctgatgc accagctgcg gcaatatgtc tctaagcacg gttatgttcc accgcagcaa
    24601 gatacacata ctgaagcgag ctggaacgac acctatgcaa aagcgcggga tgtccaggca
    24661 ctggacccgg atacattgcc gaacacctat ctgaaatatt atctcttccc ggattatgtc
    24721 gttcagcatt ccaaccctga acatactcgc gcgaatgagg taatggaaca tcgcgagaaa
    24781 caggttttcg atgcttgccg cgccattacg gcggcaggaa attcagcggc gggcaagctg
    24841 gaaattgacg aacatgcgtc atacatcgtc gatctggcgg cggcaattgc cttcaacact
    24901 caggagcgga tgttgctgat tgtgcctaac aacggggcaa tccataactt tgatgatgaa
    24961 gctatggtcg agatcccgtg tctggtcggg cacaacggac cagaaccact ggtggtcggc
    25021 gatatcccgc agtttcagaa agggttaatg agtcagcaag tggcggtgga aaaactggtc
    25081 gtggacgcct gggaacagcg ttcatatcag cacctgtggc aggcgattac gttgtcgaaa
    25141 acggtaccga gcgcctcggt cgccaaagct attctggatg agttgctgga ggccaacaaa
    25201 gcgtactggc cagagttacg ttaattgtgt ggactggcat ccgttgggtg ctggtccgta
    25261 ttctttagca cggctgaagg cgcataacca aatttatgtt taaaggcttt gctgaaatga
    25321 aaggagtcga agaagttgag cgtatcagcg acttcgccaa ccgagtttcc ttgttgcttt
    25381 aatagcgata gcgccagatc cagacgcgca tcgaggtaat attctttcgg cgtctttccg
    25441 gtatagcgta aaaacagacg acgcaaccag gcttcgctac aggggatcgt ggcagccata
    25501 tcggctacgc tccagcgttg ttgcagactg gcatgtaaag tggcaattaa tttttcaatt
    25561 tgccgccgtt gtggatcttt tttaccgtct gcgtacataa gacaaatcca ctgataaata
    25621 attttcgtca gaaaagcgac tgccagatta ttttttattg cttctggtga agttattaac
    25681 tcagcaactt cggtgagttc ctgattataa atttcgccat tgtaaataac gctttgctga
    25741 ccgatgggaa tatccatcat actggtgggg gtaaattcca tccagtattg ttcccagact
    25801 aaaccttcac agtgataaga gtgaatatcc attggcttta aaaatataat acagttgcca
    25861 tgcagtgtta tttgttcgcc attctttaaa agaatcttcc cacatccttg tacggtatac
    25921 accgcaaccc atgagttggc gataagcggt ttcttcttgt cccgtggcca gatcacccgg
    25981 taatgctcgt cggcgtaggt atgccagagc gagatcagag aaattccgct ggagatcaca
    26041 ttttcgttaa tcaataaagg aatattgtat ggagtttcgt tttttacatc tgaagtttgc
    26101 aattttccat tcattttaat atccctccgg cgaattatgc tcacaacctg aacttaacag
    26161 aaaacaatcc gttcagggat acgacgtttg tattttaaga atctgactgc ctgacccgac
    26221 gcattttacc tctccctata ttcatgcgtc cgggacataa acataaataa gggctatgag
    26281 atgaattcgt tacaaatctt gagttttgtc ggttttacgc tgctggtggc gatcatcacc
    26341 tggtggaagg ttcgcaaaac agataccgga tcgcaacaag gctattttct tgccggacgt
    26401 tcactaaaag cgccggttat tgccgcttcg ttaatgttaa ccaacctttc cacggaacaa
    26461 ctggttggtc tttccgggca ggcctacaaa agcggcatgt cggtgatggg ctgggaagtg
    26521 acttcagcgg tgacgctgat cttcctcgcg ctaatctttt taccgcgcta tctgaagcgc
    26581 ggcattgcca ccatccccga ttttctggag gaacgttatg ataaaacgac gcgtatgatc
    26641 atcgacttct gcttcctcat tgccaccggc gtctgctttc tgccgattgt tctctactcc
    26701 ggcgcgttgg cgctcaacag cctgtttcac gtcggggaat cgctacagat ttctcacggt
    26761 gcggctatct ggctactggt aattttgctt ggtctggcgg gaattttgta tgcggtgatc
    26821 ggcggactgc gcgcaatggc agtggcggac tccatcaacg gtattgggct ggttatcggc
    26881 gggttgatgg tgccggtatt tggcttgatc gcgatgggca agggcagctt tatgcagggc
    26941 attgagcaaa ttaccaccgt tcacgccgag aaattaaact cagtcggtgg cccgaccgat
    27001 cccttgccga ttggcgcggc atttaccggt ttaattctgg tgaacacctt ttactggtgt
    27061 acaaatcagg gcatcgtgca acgcacgctg gcgtcaaaaa gcctggcgga agggcaaaag
    27121 ggggcgctgt taacggcggt gctgaaaatg ctcgacccgc tggtactggt gctgccaggg
    27181 ttgattgcgt ttcatctgta tcaggatcta ccgaaagccg acatggccta cccgacgctg
    27241 gtcaataacg ttctgccagt gccactggtg ggtttcttcg gcgcggtgtt atttggtgca
    27301 gtgatcagta ccttcaacgg ctttctgaat agcgccagta cgttattcag tatgggtatt
    27361 taccgtcgca tcattaacca gaatgccgag ccgcagcagc tggtcaccgt cgggcgcaaa
    27421 tttggtttct ttatcgctat cgtttcggtg ctggtagcgc cgtggatcgc caacgcgccg
    27481 caggggctgt atagctggat gaaacagctc aacggcattt acaacgtgcc gctggttacc
    27541 atcatcatta tgggcttttt cttcctgcgc atcccggcgc tggcggcaaa agtggcgatg
    27601 gggattggca taatcagcta catcaccatc aactatctgg tgaagttcga cttccatttc
    27661 ctctatgtgc tggcctgtac gttctgcatc aacgtggtcg tgatgctggt gatcggtttt
    27721 atcaaaccgc gcgccacgcc gttcaccttc aaagatgcgt ttgcggtgga catgaaaccg
    27781 tggaaaaacg tcaagatcgc gtcaattggc atcctgttcg cgatgattgg cgtctatgcc
    27841 gggctggctg aattcggcgg ctacggtacg cgctggttag cgatgatcag ttatttcatt
    27901 gccgccgtag tgattgtcta cctgattttt gacagctggc ggcatcgtca cgacccagcc
    27961 gtaaccttta ctcccgacgc gaaggatagc ctatgaaacg ccccaatttt ctgttcatca
    28021 tgaccgatac ccaggccccc aatatggtcg gttgctatag cggtaaaccg ctgaatacgc
    28081 aaaatattga tagtctggcg gcggaaggta ttcgctttaa ttccgcctac acctgttcac
    28141 cggtttgtac accggcgcgc gccgggctgt ttaccggtat ctacgctaac cagtccgggc
    28201 cgtggaccaa caacgtcgcg ccgggtaaaa acatctccac tatggggcgc tactttaagg
    28261 atgccggcta tcacacctgt tacatcggca aatggcatct cgacggtcat gactatttcg
    28321 gcactggcga gtgtccgccg gagtgggacg ctgattactg gttcgatggg gcgaactatc
    28381 ttagcgaact gacggaaaaa gagattagcc tgtggcgcaa tggcctaaac agcgtcgaag
    28441 atttacaggc gaaccatatt gacgaaacct tcacctgggc gcaccgcatc agcaatcgag
    28501 cggtggattt tctgcaacag cccgcgcgcg ccgaggaacc cttcctgatg gtggtttcgt
    28561 atgatgagcc gcatcacccg ttcacctgtc cggtggagta tttagagaaa tacgctgatt
    28621 tttactacga gctgggcgag aaagcacagg atgacctggc gaacaaacca gaacatcacc
    28681 gcttatgggc gcaggcgatg ccatcgccag tcggtgatga cgggctttat caccatccgc
    28741 tctattttgc ctgtaatgac tttgttgatg accaaatcgg acgggtcatc aatgccttaa
    28801 agccagagca acgtgaaaat acgtgggtta tttatacctc cgatcacggc gaaatgatgg
    28861 gcgcacataa gctgatcagt aaaggggcgg cgatgtatga cgacatcacc cgcattccgc
    28921 tgatcatccg ttcgccgcaa ggggagcggc gacaggtcga tacgccagtc agtcatatcg
    28981 atttactgcc gacaatgatg gcgctggcag atattgaaaa accagagatt ctgccggggg
    29041 aaaatatcct tgcagtgaaa gagccacgcg gcgtgatggt ggaatttaac cgctacgaga
    29101 ttgagcatga cagctttggc ggttttattc cggtgcgttg ctgggtgacg gatgacttta
    29161 aactggtgct caacctcttc accagtgatg aactttacga tcgccgtaat gatccaaatg
    29221 aaatgcataa cctgatcgat gatgcccatt ttgccgacgt tcgcagcaaa atgcatgatg
    29281 ccttattgga ttatatggac aaaattcgcg atccgttccg cagttaccaa tggagcctgc
    29341 gtccgtggcg taaagatgca cagccgcgct ggatgggggc atttcgtcca cgcccacaag
    29401 atggttattc gccagtggta cgcgactatg acaccggcct gccgacacaa ggggtgaagg
    29461 tggaagagaa aaaacagaag ttctgacgat aatgcccgct gatgaacacc cggcgggcat
    29521 tattcaggct gcttaaacca gtgaaccgcc agtcaggaag gaaaagataa ggaatatcag
    29581 caaaatgctg cacacgatgc cgataccaaa aagcaaggta tggaaagcca gcgtcccgcc
    29641 gccaaaaacc cgcgcgccgc gaatgcccca gcgattttcc ttacgcatac cgccaataaa
    29701 tgcgcaaagt agcgcagcaa tggccagtgc gataccgctt tgatcgagga ttttatcgac
    29761 atccacgtcc tcttttctct ccacggtggt aattttttcg ccttttattc cggcgagaag
    29821 acctctttta atttcagccg ttttgtccgc cacgatgctt tcaagcgttg gcggtggtgc
    29881 ggaaaaaggg ccgaatgaaa agtggataat ccccatcatt aaagcgatgg caccgaatag
    29941 catgcccaat gaactgattt tattttgcgc tatgataccc atctgacctc cttgtagcgg
    30001 atggcaaaag catatcagga aacgggcgga cgaaattctt aaaaaaaacc gctaaaggta
    30061 gagcgttagt gaaattgcaa aggagcaaca atgaaaattt cccgcctcgg agaagcgcct
    30121 gattaccgct tctcgctggc taatgagcgt acttttctgg cgtggatccg tactgcacta
    30181 ggatttctgg cggcgggggt tgggcttgat caacttgcgc cagatttcgc cacgcccgtc
    30241 attcgcgaac tgctggcgtt gttgttgtgt ctgttttccg gcgggttagc gatgtatggc
    30301 tacttgcgct ggttacgcaa tgaaaaagcg atgcgtctga aagaggacct gccctacacc
    30361 aacagcttat taatcatcag cttaatattg atggtcgtag cagtgatcgt tatgggactg
    30421 gtgttgtatg ccggatagcc gcaaagccag acgcattgcc gacccagggc tgcagccgga
    30481 gcgtacatca ctggcgtggt ttcgtaccat gctgggctac ggcgcgttga tggcgttggc
    30541 tatcaaacac aactggcacc aggcgggcat gttattctgg atttcgattg gcatcctcgc
    30601 catcgtggcg ctgatcctct ggcactacac tcgtaatcgc aatttaatgg atgtcacgaa
    30661 tagcgatttt tcccaatttc acgtagtccg tgacaaattt ttgatctccc tcgcggtgtt
    30721 atctctcgca atactgtttg ctgtaacgca tatacatcaa cttatcgtat ttattgagag
    30781 agtcgcatga caggaagtca ggtcattgat gctgaagagg acaggcataa actggttgta
    30841 gagtacaagg atgctctgca acctgccgat ttttatcaca atttcaaaca acggggcatc
    30901 cgctccgtac agcttattcc ccatcttgaa tttgatgacc ggggcgatat gacggccgct
    30961 tcggtgacag cagagctgtg ggggaaattt ttaattgccc tgtttgagtg ttgggtgcgg
    31021 gcagatatca gtcgtatctc gattgagctt tttgacgcca cattacaaaa atggtgtggc
    31081 agcgaaaacc cgcaaccgcg ccgtggctgc caggcgtgcg actggcatct gctgtgtccg
    31141 cacgctcggg aggagacgcc agacagcgtg ctttgtgcgg gctatcaggc gttttattct
    31201 tactctgcgc cgcatatgcg ggtgatgcgt gatctgataa agcagcatcg ttctcccatg
    31261 gagctgatga cgatgctgcg gtgacgtgag cttaaacggg ctgcccctga tgcgacatcc
    31321 gcgtcgccag cggtagccag cacagcacga tcaacaatcc cattaaggtc atcaacaacc
    31381 ccaggctgcc ctgaccggtt tgcggcaaca tcgcagagag cgacgccagc acgccggaac
    31441 caatgttttg tagaccgccg accagcgcgc cagccgtgcc cgccaggaaa gggaacggct
    31501 ccatcgcgcc gctggtcgcc agcggaaaca gcatcccggc accgaaaaag aacagcgcgg
    31561 cgggaacgag cagcgtccag acattcatca cgccaaacca gtcggggatc cacatcagta
    31621 agccagccag caggcagcag ataaccgact gccacatcaa cgttgagaag cgtttattag
    31681 gacgtccggc aaaccatgcc ccaaaaaatg ccgccggaat cggcagaata aacaaaatac
    31741 tgaccgtcat actgctcagc cctaacaccg cgcccatcag cacgccggag caggcttcaa
    31801 aggcggcaat cccggccaga ccgccaatca gcatcagcaa ataacagtta aaaccgctgt
    31861 taccgaaaag cgttttataa ctggtaagca ggcgcgtgcg cggtgcgtcg accggacgcg
    31921 tttccggcat ccagcgggcc atactgaagg tcacaccggc acaaagtacc aacaaaaaga
    31981 gataacaggc gcgccagttc cacatcgtat ccagcagacc gccgattagc ggtgcgagca
    32041 acggactgac aagaattccc atgtttaaca ggctgttagc gtggcgcaac tgtgtccgtt
    32101 catataaatc acgcggcaaa gtacgcgcca ttacgccgcc aacgccggtg cccatcccct
    32161 gcatcgcgct ggcggcaatc aatactgtca aactggaggt cgtgaccgcg accagcgttg
    32221 ccagcataaa aatggacatt ccgacgagga tcaccggtcg gcgacccaca cggtcggaaa
    32281 tcgggccata aaacagctgt gagacaccgt aagtcagcag ataagcgccc attacgctct
    32341 gcaccgcccc ttcacgaacg ttgagatcgc gcgccatatc ggcaatagct ggaatataaa
    32401 tggtttgcgc catctgaccg acggccacga gtaataccaa catcaataac aaattgatgt
    32461 ttctatgcct tttcattatc acggatgctt ttataaaaat aattagaata agtcactacg
    32521 cattcccata aatgcgggag aggaatctat catattgata aatgttagcc agatatacgc
    32581 caggaatggg gaattgttta gcggcagaat atgtaaacaa aagcggcaat aaatgttgcc
    32641 gggagggatg gtaggtcgga taagatgcgt cagcatcgca tccgacacca acccacacgc
    32701 taaatataaa atgggagcgg ttacccgctc ccctttagtg cgacttgaat ctgaattact
    32761 tcaggtattt cagaacagca tcaagcagtt gcagtgctgc aacaatgagt ttgaggataa
    32821 gaatggcgat atccaccagg ctcatacgcg tctcctgtgg ttcaggagca ctgccagctg
    32881 acgtaccttt ccgctgcctg ttgccagctg ttgtgcacgc ggtatctcaa ggagaaacgt
    32941 tgtgctcgtt gacataacac agtgtgctca cgcggggtta aggcgctgac ggcaccaccc
    33001 gtttcagcca ggacttagtg cgccgggtaa cgatgcggcc cccgcataac acattgcgta
    33061 cagtgatatt atagaagctt tctgtataaa taaacagtaa tatttggaca aaacgcaaac
    33121 tgtgatcgat actcaactat gcactaaata cgtcaaaatt cgtgccgaaa ttgcgcgttt
    33181 tgcgcggaac acgtatactt tctgtgttga cataatacag tgtgctttgc ggttaccagc
    33241 cgcgggcggc tgacgaaacc tcgctccggc ggggtttttt gttacctgca gtacagtaca
    33301 aaacgtgatc aacccctcaa ttttcccttg ctgaaaaatt ttccattgtc tcccctgtaa
    33361 agctgtgctt gtataaatat tgttaaacac aaaaccaaca aggtccccaa tggctacttc
    33421 catgctcaac gcaaaactac taccaactgc gccatccgcc gcagtggtcg tcgtgcgtgt
    33481 ggtggtggtc gtcggcaatg cgccgtaggg actggaacaa cacacgattc caaaaccccg
    33541 ccggcgcaaa ccgggcgggg tttttcgttt aagcacctcc cggaaagtcg gcccagaaga
    33601 aaaggactgg agcatggcaa gttcgggcac atcatcgacg cgtaagcgct ttaccggcgc
    33661 agaatttatc gttcatttcc tggaacagca gggcattaag attgtgacgg gcattccggg
    33721 cggttctatc ctgcctgttt acgatgcctt aagccaaagc acgcaaatcc gccatattct
    33781 ggctcgccat gaacagggcg cggggtttat cgctcaggga atggcgcgca ccgacggtaa
    33841 accggcggtc tgtatggcct gtagcggacc gggggcgact aacctggtga ccgccattgc
    33901 tgatgcgcgg ctggactcca tcccgctgat ttgcatcact ggtcaggttc ccgcctcaat
    33961 gatcggcact gacgccttcc aggaagtcga cacctacggc atctctatcc ccatcaccaa
    34021 acacaactat ctggtcagac atatcgaaga actcccgcag gtcatgagcg atgccttccg
    34081 tattgcgcaa tcaggccgcc ctggcccggt gtggatagac attcctaagg atgtgcaaac
    34141 ggccgttttt gagattgaag cacagcccgc ggtggcagaa aaagccgtcg cgcccgcctt
    34201 tagcgaagaa agcattcgtg acgcagcggc aatgattaat gctgccaaac gcccggtgct
    34261 ttatctgggc ggtggtgtga tcaatgcgcc tgcgagagtg cgtgaactgg cggagaaagc
    34321 gcagctgcct accacaatga ctttaatggc gctgggcatg ttgccaaaag cgcatccgtt
    34381 gtcgctgggt atgttgggga tgcacggtgt gcgcagcacc aactatattt tgcaggaggc
    34441 ggatttgttg attgtgctcg gtgcgcgttt tgatgaccgg gcgattggca aaaccgagca
    34501 gttctgcccg aatgccaaaa ttattcatgt cgatatcgac cgcgcagagc tgggtaaaat
    34561 caagcagccg cacgtagcga ttcaggcgga tgttgatgac gtgctggcgc agctgatccc
    34621 gcaggtggaa gcgcaaccgc gtgcagagtg gcaccagttg gtagcggatt tgcagcgcga
    34681 attcccgtgt ccaatcccga aagcgtgcga tccgttaagc cattacggcc tgatcaacgc
    34741 cgttgccgcc tgtgtcgatg acaatgcgat tatcaccacc gatgtgggtc agcatcagat
    34801 gtggacggcg caggcttatc cgctcaatcg cccacgccag tggctgacct ccggcgggct
    34861 gggcacgatg ggttttggcc tgcctgcggc gattggcgcg gcgctggcga acccggatcg
    34921 caaagtgttg tgtttctccg gcgatggcag cctgatgatg aatattcagg agatggcgac
    34981 cgccagcgaa aaccagctgg atgttaaaat cattctgatg aacaacgaag cgctggggct
    35041 ggtgcatcag caacagagtc tgttctacaa gcagggcgtt tttgccgcta cctatccggg
    35101 aaaaatcaac tttatgcaga ttgccgccgg attcggcctc gaaacctgtg atttgaataa
    35161 cgaagccgat ccgcaggctg cattgcagga aatcatcaat cgccctggtc cggcgctgat
    35221 ccatgtgcgc attgatgccg aagaaaaagt gtacccgatg gtgccgccag gtgcggcgaa
    35281 tacagaaatg gtgggggaat aagccatgca aaacacaact catgacaacg tgattctgga
    35341 actcaccgtt cgcaaccatc cgggcgtaat gacccacgtt tgtggccttt ttgcccgccg
    35401 cgcttttaac gttgaaggca ttctttgtct gccgattcag gacagcgaca aaagccatat
    35461 ctggctactg gtcaatgacg accagcgtct ggagcagatg ataagccaaa tcgataagct
    35521 ggaagatgtg gtgaaagtgc agcgtaatca gtccgatccg acgatgttta acaagattgc
    35581 agtgtttttt cagtaaggag cgacattaaa ccgctcgggg gagttactga tgagcgattt
    35641 ttgatagatc cgtgcagata tatagggtga aattgtgcca gagaccatct caggcacaat
    35701 ttattagccc aggacgttaa tttaagtcgc agcaccggta acctgggggg gacagttagg
    35761 gctaaaattt tcagcgatgg tgttaaattc tgattgctgt ctataccagt ggtcatgacc
    35821 agcattctat taattaatgg ttcatcgtga ggtatattat ggcaagtcct ataaacagcg
    35881 gaataatgat ggcaaatctc tgcccttcca cctttttgga taaaaataga aatgtcgctg
    35941 ctgagcttga tattaaaaat aacgagaaaa aatattcacc tggcagtaat ttcgcaaaat
    36001 ggatgttgca ggaaataaag cgcttaatac taaacatcat gtccggttct cggagtatta
    36061 acaccgatat tcttgactat ttccacccta tgccaggcac ggaaaataat ggtaatcgta
    36121 cctgggtggc ggcaactgga gaggatgaat atatcgaaat caaacaaaca ggtgataagt
    36181 cttttaatat tacactggtt ggcagagata aaccttcacg aaaagaaatt ccatattcag
    36241 gagtggctgt agcgactatt attaaatcgt tgtctgaaaa aacttctgcg ttagaaacac
    36301 attccgctga cacggttttg aggaagaaat tagttaatag tattgttatg aagaatacgg
    36361 attttaatta cgaaatt
//
LOCUS       DAZFBR010000037        36198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFBR010000037 DAZFBR010000000
VERSION     DAZFBR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.037"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(136..1050)
                     /gene="ydeQ"
                     /locus_tag="ACHJZ2_002066"
     CDS             complement(136..1050)
                     /gene="ydeQ"
                     /locus_tag="ACHJZ2_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4568180.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGTTTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            complement(1110..1613)
                     /gene="ydeR"
                     /locus_tag="ACHJZ2_002067"
     CDS             complement(1110..1613)
                     /gene="ydeR"
                     /locus_tag="ACHJZ2_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4568181.1"
                     /translation="MKRLHKRFLLATFCALLTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(1626..2156)
                     /gene="ydeS"
                     /locus_tag="ACHJZ2_002068"
     CDS             complement(1626..2156)
                     /gene="ydeS"
                     /locus_tag="ACHJZ2_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4568182.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            complement(2170..4821)
                     /locus_tag="ACHJZ2_002069"
     CDS             complement(2170..4821)
                     /locus_tag="ACHJZ2_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4568183.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLVDASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGATFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSTLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            complement(4863..5573)
                     /locus_tag="ACHJZ2_002070"
     CDS             complement(4863..5573)
                     /locus_tag="ACHJZ2_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4568184.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSNLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            complement(5934..6497)
                     /gene="fimA"
                     /locus_tag="ACHJZ2_002071"
     CDS             complement(5934..6497)
                     /gene="fimA"
                     /locus_tag="ACHJZ2_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4568185.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(6931..7200)
                     /locus_tag="ACHJZ2_002072"
                     /pseudo
     CDS             complement(6931..7200)
                     /locus_tag="ACHJZ2_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(7471..8535)
                     /gene="hipA"
                     /locus_tag="ACHJZ2_002073"
                     /pseudo
     CDS             complement(7471..8535)
                     /gene="hipA"
                     /locus_tag="ACHJZ2_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            complement(8651..9649)
                     /locus_tag="ACHJZ2_002074"
                     /pseudo
     CDS             complement(8651..9649)
                     /locus_tag="ACHJZ2_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
     gene            complement(10180..11772)
                     /gene="lsrK"
                     /locus_tag="ACHJZ2_002075"
     CDS             complement(10180..11772)
                     /gene="lsrK"
                     /locus_tag="ACHJZ2_002075"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO4568186.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSTIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(11851..12804)
                     /gene="lsrR"
                     /locus_tag="ACHJZ2_002076"
     CDS             complement(11851..12804)
                     /gene="lsrR"
                     /locus_tag="ACHJZ2_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO4568187.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            13053..14588
                     /gene="lsrA"
                     /locus_tag="ACHJZ2_002077"
     CDS             13053..14588
                     /gene="lsrA"
                     /locus_tag="ACHJZ2_002077"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO4568188.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAMLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            14582..15610
                     /gene="lsrC"
                     /locus_tag="ACHJZ2_002078"
     CDS             14582..15610
                     /gene="lsrC"
                     /locus_tag="ACHJZ2_002078"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO4568189.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            15610..16602
                     /gene="lsrD"
                     /locus_tag="ACHJZ2_002079"
     CDS             15610..16602
                     /gene="lsrD"
                     /locus_tag="ACHJZ2_002079"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO4568190.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            16614..17636
                     /gene="lsrB"
                     /locus_tag="ACHJZ2_002080"
     CDS             16614..17636
                     /gene="lsrB"
                     /locus_tag="ACHJZ2_002080"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO4568191.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            17663..18538
                     /gene="lsrF"
                     /locus_tag="ACHJZ2_002081"
     CDS             17663..18538
                     /gene="lsrF"
                     /locus_tag="ACHJZ2_002081"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO4568192.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            18562..18852
                     /gene="lsrG"
                     /locus_tag="ACHJZ2_002082"
     CDS             18562..18852
                     /gene="lsrG"
                     /locus_tag="ACHJZ2_002082"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO4568193.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            18909..19172
                     /locus_tag="ACHJZ2_002083"
                     /pseudo
     CDS             18909..19172
                     /locus_tag="ACHJZ2_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(19173..19256)
                     /locus_tag="ACHJZ2_002084"
                     /pseudo
     CDS             complement(19173..19256)
                     /locus_tag="ACHJZ2_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19453..20904)
                     /gene="uxaB"
                     /locus_tag="ACHJZ2_002085"
     CDS             complement(19453..20904)
                     /gene="uxaB"
                     /locus_tag="ACHJZ2_002085"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO4568194.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(21131..22549)
                     /locus_tag="ACHJZ2_002086"
     CDS             complement(21131..22549)
                     /locus_tag="ACHJZ2_002086"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4568195.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(22688..23047)
                     /gene="yneG"
                     /locus_tag="ACHJZ2_002087"
     CDS             complement(22688..23047)
                     /gene="yneG"
                     /locus_tag="ACHJZ2_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO4568196.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(23047..23973)
                     /gene="glsB"
                     /locus_tag="ACHJZ2_002088"
     CDS             complement(23047..23973)
                     /gene="glsB"
                     /locus_tag="ACHJZ2_002088"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO4568197.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(24037..25425)
                     /gene="sad"
                     /locus_tag="ACHJZ2_002089"
     CDS             complement(24037..25425)
                     /gene="sad"
                     /locus_tag="ACHJZ2_002089"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO4568198.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMDDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            25526..26407
                     /gene="yneJ"
                     /locus_tag="ACHJZ2_002090"
     CDS             25526..26407
                     /gene="yneJ"
                     /locus_tag="ACHJZ2_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4568199.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            26485..27600
                     /gene="yneK"
                     /locus_tag="ACHJZ2_002091"
                     /pseudo
     CDS             26485..27600
                     /gene="yneK"
                     /locus_tag="ACHJZ2_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            27751..28941
                     /gene="ydeA"
                     /locus_tag="ACHJZ2_002092"
     CDS             27751..28941
                     /gene="ydeA"
                     /locus_tag="ACHJZ2_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO4568200.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(28966..29631)
                     /gene="marC"
                     /locus_tag="ACHJZ2_002093"
     CDS             complement(28966..29631)
                     /gene="marC"
                     /locus_tag="ACHJZ2_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO4568201.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            29843..30277
                     /gene="marR"
                     /locus_tag="ACHJZ2_002094"
     CDS             29843..30277
                     /gene="marR"
                     /locus_tag="ACHJZ2_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO4568202.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQNLHQELTKNLTADEVATLEHLLKKVLP"
     gene            30297..30680
                     /gene="marA"
                     /locus_tag="ACHJZ2_002095"
     CDS             30297..30680
                     /gene="marA"
                     /locus_tag="ACHJZ2_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO4568203.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            30712..30930
                     /gene="marB"
                     /locus_tag="ACHJZ2_002096"
     CDS             30712..30930
                     /gene="marB"
                     /locus_tag="ACHJZ2_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO4568204.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(30961..31860)
                     /gene="eamA"
                     /locus_tag="ACHJZ2_002097"
     CDS             complement(30961..31860)
                     /gene="eamA"
                     /locus_tag="ACHJZ2_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO4568205.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            32055..33242
                     /gene="ydeE"
                     /locus_tag="ACHJZ2_002098"
     CDS             32055..33242
                     /gene="ydeE"
                     /locus_tag="ACHJZ2_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO4568206.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(33283..33678)
                     /locus_tag="ACHJZ2_002099"
     CDS             complement(33283..33678)
                     /locus_tag="ACHJZ2_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO4568207.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            33773..34744
                     /gene="ftrA"
                     /locus_tag="ACHJZ2_002100"
     CDS             33773..34744
                     /gene="ftrA"
                     /locus_tag="ACHJZ2_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HFO4568208.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            34852..34947
                     /gene="mgtS"
                     /locus_tag="ACHJZ2_002101"
     CDS             34852..34947
                     /gene="mgtS"
                     /locus_tag="ACHJZ2_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO4568209.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            34947..35051
                     /gene="mgtT"
                     /locus_tag="ACHJZ2_002102"
     CDS             34947..35051
                     /gene="mgtT"
                     /locus_tag="ACHJZ2_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO4568210.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(35166..36056)
                     /gene="dgcZ"
                     /locus_tag="ACHJZ2_002103"
     CDS             complement(35166..36056)
                     /gene="dgcZ"
                     /locus_tag="ACHJZ2_002103"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO4568211.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         9424 a         8911 c         8367 g         9496 t
ORIGIN      
        1 tttcaatata aaaaataaga ctctggcttc aattgtgcgc ggattttctt acaagtgtag
       61 gataatagcc ttcacaaaat aattatccgc gcaattttcg tgaatggagc gcatagacta
      121 aggtcaaaat cacaattatt catagataaa agtgacacca atgactgact ggacggttcc
      181 ggctgaaact ttgttccctg tttgtccata ggttgcgctt aaacccagag gtactttgct
      241 tttatttact gtccccaggg agacattttc tcccgtagcc agtatttttc cattacgcat
      301 cagagagaca cccactcccg atgcttttgt tgcatctggt gctgtgttag caaacacttg
      361 tctagcactg tcagtggtag tacccgaaag ataaaatgag agcttttgtt cgcttgagca
      421 atatacgcca agggggattt ctgcgcttcc aggaaagtcc gggagattca cggtgacatt
      481 acgactatca acagtgcagc cgcctgttgg cataacaaca ctgttatttg aaataatatt
      541 ccacgtaaaa ttacgcggat ttccgcttcc taacgtggca attttataca tatgaatacg
      601 ggcaataact tccccggctt ttatgacaac tccaccggcc gccccgactg gggtaatata
      661 gagttttaac ggtaatggca tcggtgtctt atcaccaata tccaatacgt tggtatttgt
      721 cgtcaacggg aaggggtagg tcacattatt ccagtacagc gatcctttat atgactgtag
      781 agagcctgcg aaagctgagc cttgtaccag gtttatatga tcagtgtcgt accagccgcc
      841 gtaatcattc cagcaactta tatgctgaga caagtctaca accagattct ggcccggctg
      901 tattacaggg tcgagattaa cataaacaga ggttgtacca gctccaatac ttgacccgcc
      961 atcgacatta caactgaagg caaaaacttt gcctgccatt aatagaagat aaataccgaa
     1021 caggaccttt atactgattg ttttacccat gatatatcct aaggttaaaa aattgattta
     1081 aaaagaaggc taagaaaatc gaatcgacgt tattgccagg tgtagatcac attgattagc
     1141 gcctcgatcg ttccctggct tgcgtttcca ttcaccgtga tagctcttgc cttaagtgga
     1201 aactgtgcat tacgagtgat ctcatcaaca ataaccgttt tgctatcgcc attttttaac
     1261 gtcgcatcct ggtcatccct aagctctatc tgaatatttt cggcagtacc ttcatttttg
     1321 taataacccg tattgtcagt tgaacctgtc acgattgccg tcactgcact tgtttcagcc
     1381 ggacaatcgg ttaatgacaa agtaatattg tgccagccag atgcagaacc aggttgttgc
     1441 agattgcgcg tataaagatc cccgagatta acgttagctt ctttggtttg aatagtgcag
     1501 ggtttagcga ctacccgacc attaacagtg atagtgacat cggcggcctg gagagttgct
     1561 gttaataacg cgcaaaacgt agctaagagg aacctcttgt gaagtctttt catatctgac
     1621 tctgattatt gaaattcaag ggtaaaggtt gccgaagccc tcaccagtcc cggattgacg
     1681 gacttctgag ttgacttcag acgagcggag taaggcaaaa tattgttctg ttctggtacc
     1741 agtgggatcc actgcatccc ggcatgaagg tcattcagtt tcaccggacg catatttccg
     1801 tccagtattt ctatacctaa accggaggcc gtattgcttc cctcatccag tttcaacaga
     1861 gtattatttt ctgcgtcctc aataccgtta aatgcaaccc gaacccccgt tgtccctttg
     1921 ctgcattcac ttaacgtaat ctgaaaaggg acggctggac ttgtgctacc ggtcgttgga
     1981 aattgtctgg cactgttttt ttggagatct acggtaaaat taagcgaatc cgatgagact
     2041 gtgcagccat aatcgaggac gcgcccgcta attttaataa cgctatctgc ggataaagca
     2101 gaataggtgg ttaaccccag acataaaccg aggaaaataa tgttattgta tttcataatc
     2161 tattgttcct tagcgacaga ttgctgtctg ctggttcagc aaggttccag gagagacttc
     2221 aggaagcttg taatcgacaa tacagtttga gtttttatca ttgccccatg aaacctgtaa
     2281 tttccctgac tgtggaagtc cagtcagata aacctgaccg ttttccgcga caatgctgcc
     2341 atttttattc tctccgtgag tgacaattgc accgaatgga acgcttttat taccgtactt
     2401 caacgtcatt aatactttcc cgccgatttg tgcattaaat gttgctctgg caatagcacc
     2461 gtgagttggg atgacagtga ccacggtttc atccagttca acattatctg caagggaatt
     2521 cgcgttaaga gcgacacgat tttctctata ttctgtcgca aatggtaata tggcatagcc
     2581 acgccagtcg gtatgaattc cggtctggtt ctctattttg acattatcag cgccaggagc
     2641 cttaaccaga accattgtgt cgcccagcgg ctgtccaaag gtgatgccat cagcatgagc
     2701 aataatccca ccactcattc cgtaataaat ctggctgctg tcaccactcc gactgtaacc
     2761 gacattagta ttgccgtaag ctccacgata attaagagta ctgtaaccac tggtgccaga
     2821 cgatgtatta cctccgtggg tgttaccgac ctgaacgcta taattcaggt tattatccgg
     2881 cagcagagtg ccataaaccc ccgatagatt ggtcatgccg cctttcaaat cgtttgacat
     2941 actgtaactg gcgtttgaat tacgaaatgc cgactgactg tctgtacgca tccaatgact
     3001 gaagggaaca ttaagcgtaa aagcgagtaa atgatcccga tcgttttgcc atatattatt
     3061 ggaatagctg taattcagcg aagtcgtaat atcaccaaac ggcacattta atccaaatga
     3121 tatttgctgg tcgctgcgtg acgtgttcca gtaactttgg cgactggcac tgaaaaatgt
     3181 cgcaccgtaa tttccaagct gctgagagat gcttatttgt tcctgaccac gcttactgta
     3241 gaacagatta aaataatcaa taaattgtgt ctgctcattg ctgtctccgg taggaggctt
     3301 gacggtgtaa ccactcattc gactgtaggc actgtcggat aagttataaa aaccttgcgt
     3361 agaatagcga tatcctgcga cctggatatt ggttcctgtc tggtagaagg atttgctgta
     3421 gacggattta accgattgcc cgctgtgacg ggtgccatct gcaagtgtcg tattggcctg
     3481 cgtgatatcg aaagaaaagg caccaaaaag tcctaaatct ttaccaatac caaggttgaa
     3541 ggcctgataa tcttctgcaa tttgcattcc gccataaggt gtccagtttc cttccagtcc
     3601 atgcatcaag ctaccttgta tgaacctggg ggagctttgc aggttatttc cactacgata
     3661 ttcccccatg gcaagcgcat aacgcgtata tccagcacgt tggagaaccg gaacagacga
     3721 ataaggaaca tataatgtct gaatactgcc gtctgcctct tttatggtta cttgcagatc
     3781 accaccattg gccgcagagt tgatatcatc aatagtaaaa gggccgggtg gaacagtagt
     3841 ctgataaaca tcgtatccgt tctgttttac actcacttgt gcggtgccac gggcaatacc
     3901 atgaatcacc ggagcgaaac catgttggct atcgggcaac atcgcttctg ttgaatttat
     3961 tttgaggcca cgaaagttca cactatcaaa aatatcgcca tcggtataac tatcacctac
     4021 cgttaaccgt gagcgtaagg gaataatgtc acgttcagcc gacgtattga tatgctgcca
     4081 tttattgctg tcagatgaat tgctactccc actgttataa ctccacgttg agttatcgcg
     4141 tagtcgccaa ctaccaatgt tgatgccact ctgtaaattc aaatatgcat agttggattt
     4201 tcctgcatta tggttactac gattattaat agaattacca ttaaaactat agtttagaag
     4261 cccagcattt attccttctt cccacagatc aggagaaaca tagccgcgag ccattctgcc
     4321 aacataaatt tgcggaacgg agagtgacag atgttgctga ccaacatcaa actcagttga
     4381 tgcgtcgacc aaacgatcct gaagtggtgt gcatgcgctg ttgtccggca catgtttttc
     4441 tgctgaatgt tccttattgt ctaataacgc agattttttt atgccaagac tgacaagaag
     4501 atctgtactc aggcatggga tgagatctgc gttattatca tctgtaataa aagtaatatc
     4561 ccgactagtc ataaattcgt cgttcaaata aatgtcgacc cgatatgtac caggtggtgc
     4621 ctcgcgtccc ttagtaaagg cggataaatc gacagattca gcaagatcat tgcttaaaaa
     4681 ccgtggatta aaataatact ctgcgtcaac gggaaatacc gcagttgcta agctaagggc
     4741 aatggtaagc cgtgcaaagc gaggcagtag taacacctga tgcatacgat atgccttaaa
     4801 tgcggcacga aaggctgtca ttattaattc cgattgagtt tattattaat taggtcactg
     4861 gcttactgca tggttgcctt aatacgcgga gttaatgcgc cgtaatcatt gatcgtctga
     4921 aaactgatat ccccggtggc agcatgcggg atatccaccg acacttcacc ttttggcggc
     4981 accatggtgt ttgggagatt gctatttcct gctttcatat ttgttacggt aatgaagtag
     5041 ggcgttggat taataagagt gagattgcta ccgctgcgac gaaaccgaag cattgcaggc
     5101 gcttcttcag gtgccattgc caggttcgtg ggccgataaa acatttttat gcggctaata
     5161 attgccagct gtaatgtatt ctcattcttc tggtcttttt ccatagcagg aatagcttta
     5221 acattaaccc agaacaggct ttcacggtcg ccgggtaact gatgattagt ggcgttgatt
     5281 atccgcagcg tattctcttt tttaccttgc atggaaaaaa gaggtggggt aatgacaaac
     5341 tgggtgtctt tttggtcacc agcattttct atccatgact gaatgagata gacactggca
     5401 ggatcattat tcgtgacagg caataaaacc tgtttctggt ttgccggata aattacccgc
     5461 gtcgcaccca gtgcgacagc tgcgttggca acggtgctat ggcaagcaaa gagcaataaa
     5521 agtaacactg tcgccataaa ggaaatagag tagggggtac gcgtagtttg catctgatgc
     5581 attctccatg tgttaagagg ataagcggga aaaccgttac atcagacaat cggtaaatgt
     5641 tgctaataca ttggcgagtc accgggtttc cagacttaat gcgtttgtaa taaaggttgg
     5701 ttctctttta ataggaaaac cttgctaaaa agtaaatatt tttaataaca gtaagtttaa
     5761 ctactgttgt ttattgaacg tttagatatt atgtgatttt tcatagcgct ccaaatacaa
     5821 accactcatc attaataatg agattcacat tggtgaattg ctcgggtgtg cgtagccatg
     5881 ccacacacac cctgttttaa tttcagatgc cgtatagcca ccttaatatt gaattataga
     5941 tattgaactt tgaaggtcgc atctgcgttt gcagtaccag ctacggactg cccgagagca
     6001 atataacgag cctggaatgg tagtatattc gtgccatcaa tcaagtcggt tttagcacta
     6061 aaagtggccc catcaagtat taatacctca cccgtacggt ccaaaatttg aataccgaca
     6121 ttttgggcag agcctgccgc tgaactctgc agcgctaacg tatcgtcatt actggtgaca
     6181 gtagtaccca ggaatgcaac agctgcatta ctggaaacat tggtatcaca atcattcagc
     6241 ttgatattga agccaacggc ggagctgagg tcacccgctt tagccaggcg agaagaacga
     6301 acctgaccca gctcaaccgt ttggttcatt gattcggaat caatcgcaca agcggcattt
     6361 accacttcac ctttaaaatg aataacaccg ccattaacag tggtcgttac tgattcatca
     6421 ccctcggctg cgcttaccgc agcagacgac atcgccaaca cagaaacgac aatcatacca
     6481 acatgtttga gtttcataat tctccaaaat ttatatttaa gtagctgaat aaagagagtt
     6541 aacaattaag tcattctgtt attgcttata aaacaagaat cattcttgat tggttgttgc
     6601 cctgagaata tagtttccag ggttattaca tttcacgaag aacgataata tctttaaatc
     6661 acaaggacac ctaatcacaa caagtttatt gctgcacttg ttgaagagag tatttgcaaa
     6721 aacattttat gtcgctaaat aacatgacga tttcattatg catgttattt ataaaccgtg
     6781 actaaacaga atattattgc gaacaaggga agtgtcagga aaggtcaagg gctactaatg
     6841 gtttatatgg ctataaatca ttagctatag ttaatgtttg cctggtgtaa ctaacaccga
     6901 attgctctaa acagataagt gcagggttta ttaaagctga ccgacagact ttcttaacct
     6961 ggtaatatct ctcttaggtg actctccaaa catccgcaaa tattcccgtc ggaaatggga
     7021 taagctttcg taaccgacag cataggctgc tgtggtgaca tcgtaatgtt cattcagcat
     7081 cgttcgcctg acttcattga gacgtagcca tttctgatac cggagtggac tcattcccgc
     7141 aagctgcctg aagtgttgat ggaaagaggg cgtgctcatt tgcacgcggt ttgccagctt
     7201 gagtcttcaa cgctgggaag atgcggcaaa cggcaaatcg gcataccagc cagcctatat
     7261 ccgtcatgca caggccaatt atgaaagagg agaagcttaa gttctggaat cctgaacgaa
     7321 taacgactac cggaaaatca cttcgtatca ctcagatacg atttaacata atatacatta
     7381 tgcgcaccaa tataaaccaa gggagaatct agtcgttggc ggtcatgatt gtcctgctca
     7441 ttaacaacga ccaaacccca tatcttactc ttacttaata ccgtattctc ggcttaaccg
     7501 accgtgcaac ctcaacacat tggtttcaac tgccgtcacc acgttctctg gaaaatctgt
     7561 cggtaatgaa gtcttcacgt tatccagtgc tgccggaatc attctggcaa agtcactcag
     7621 gatttcatgc atctgcactt ccgggaatct cagcaccttt gctgtcgcca aaaaatgtcg
     7681 cggataaatt ttatcgattg ccgttttttt gcctttggat gcgttaagcc ccattgccag
     7741 tttgagatcg ctgatgtgta ttcccgtacc gccaaggacc ggaaatgctg aaatgatgtc
     7801 gtaaaatggc gtgagtcgat aactgccgcc agcctgaata aatacggaga agttttttgc
     7861 atggccatcc gttgcgccaa tcaaccactg gaagacctgg aatttcataa aatcataacg
     7921 atcgcgaagc gcctcgctga accccatcaa aaaagccatg atccgcgcga tgcctgggcc
     7981 tccatctgat tcatatttca ccgatgaagg taaaccgaat gtctgacaca tatcctcctg
     8041 tggcaagcga agtaaaaccg ttcgctcagc attccaacgc ctgtcaaaac gttcgaccgc
     8101 taacgcgcgc acatttcccg ctttaatgat ttctgcgtcc gggacattca acccaagttc
     8161 tttcgccagc agcagacagt aatactcatt atcaacgctt tggctgagat cgagcgtcgc
     8221 attgggctgc ctgatttccc caatcggtaa tttaatgatg tgcgtcgtcg gcgttattcc
     8281 tttcggaatg caccagtcat tgcctattct gagcagtgct gtcttctcct gtgcgccagc
     8341 aaccgagatg cgaaagtcat tttcttctct aatcatgcct agcgggatat ctgctttata
     8401 agccgttaat acttcttcaa gtctgacttc agtaagcttt tcccatgcca ttatcggacg
     8461 cgttacggtt tcgtcttcgg gtattaacgt cacggcacca acgctgtctc gccctatttc
     8521 tgacaataaa tcaaactgta cgggataaac gctgcgatat ccgcgatcgc ggataaaagg
     8581 gaggctttaa aagccttttg aagttggcaa tattaagccg catgccatct cgacatgcgg
     8641 cttatactgt atcggtcacg ttgacgacac cgtgggaacc gtcctgaacg cccaccaatg
     8701 aatattcttt gttattaatt agtccattat tggaaatatt gactgcgcca ttaccgaaat
     8761 atccattata gagtctgcgt accgtgatga cagagtcttg atcctgcaca ttcagggttc
     8821 cgacaccggt ggcattgcca ccgataatcg tattttcagc cgtaatcagc ccaccctcgc
     8881 gaatagtcgc ctccccagtt ccttgattac caatttgaaa ttcaattgag gaatcattat
     8941 tttttattag ccactcgcca cccttttcaa cgacaacctt accattactg ttcgcttgat
     9001 atcctaaaat ggcgactatt gaactcgtga cgtaaccctt atccgtaata tttaatgagc
     9061 ccgtaccgta actccctatt tcgaataatt cggtcgtcaa aaccgagtcc tctccctcaa
     9121 cattaaccgt cccgacgcct gcagcttgag tacctaatct taaatagcct ccatcgacgt
     9181 gacccttctg tttaatattc agcgtccccg ttccggattg acccacattt aaaggccttg
     9241 cattatttcc gctatcatac aatcgccagg tgccacccaa aacattaacg gtaccctccg
     9301 aatcctcatt accgccaatg acgctggtag taatagtggt taaggaggca ttaccgccag
     9361 ccaaaatatt aagcactcca gtgccatacc agccaaccag gtaggcatca taagcaacat
     9421 cagtatcaat attggtaatc tgaccattat caacttccag actcgcacca gacactgaac
     9481 cgcttagtgc cagcaaaaca cccagcgtca atctactgaa tttcgttgtc agtccagagg
     9541 actttcgcaa attaaccgtt gatgttttac ctaccctgcg agttaattcc gagcaggcct
     9601 gaaatacctg tagagtgcaa ttccatatca cgcgatagat tctattcata taaacgctcc
     9661 aaatacaaac aatacagcca cgaaagagat gagtcgtcag acattaaaaa tgaaatttat
     9721 taaattgtca gaggtctgta ttgagtgtta gttggaggtg ggaaagtgtc aatttggcta
     9781 acttgctaat actttcgaat tgatagatta aaaattgcat agttcttatt ttatttaaaa
     9841 atgaactatg agttattgtt gcttaataag aaaactgttt ctttcagtag gaaaattatc
     9901 atcatcatat ttaacaaaga atagcaccaa ttgctaaaaa tcgaagttta ttaaacccct
     9961 tttgcatcca ttaatgtctt taggaatatt cgttataaaa taagggttat gcttaatgct
    10021 ttaagaaaaa tagcaatttc cccttgaata tagtactggt gatgaaacga tgaatctgca
    10081 agctggcatt taacaagcca gctctaaaag aagggaaata agaataacta tactcaaaaa
    10141 ctaacagcca cggtcatcat gatgtggctg tcaatgaaac tataacccag gcgctttcca
    10201 taacgacgtc gtcagtccat gatcaaccag ccccagctga tcctgataaa ctgcctgcca
    10261 cttatcgcgt gaatcctgat aaagttcatg cttttccggg tctggtgtgt gcgtccgttc
    10321 ccagcgaacc aggcgttctc cggtttctgc cattgatgaa aaaattccgg caccgacgcc
    10381 agctgcaatg gcacatccta atgcagtggc ttctttgacc accggaatat tgacgggtaa
    10441 tcccgagaca tcagcgagaa tttgactcca taatttccct tttgaacctc cgcctgcaaa
    10501 gactaacgat gaaggatgaa tagtcgaaaa atcagcaatt tgctgcaagt tacacgctga
    10561 tacaatcgcc gcattttctt ccagcgcacg gaacaatgtc gctttgttac atttatccgg
    10621 atcaatggac aaattaataa aggaaggcgc agcgtgatac caggttttaa agcgcattct
    10681 gtcggagaag atcggcatta cgccccacga cccaggcggc acccgactgg ccatctcttc
    10741 cagcagcgta taggtgtcga tgcctaaacg ttcggcaatc agtttttctt cggcacagaa
    10801 agcatcgcgg aaccagcgca tggtgagtcc ggtaaaaaag cttatagatt cagcttgtac
    10861 catgccagga ataacatgag gattaacgcg cacgttcatt tctgggtctg tcaccggcgc
    10921 ggctaaattt acaacttgct gccagaatgt gccgccaaga accgcggttt gtgccggacg
    10981 cacaacgcct aaccccaggc aaccaagctg cacgtcgcct cctccaacga ccaccggagt
    11041 gcccaccttc agaccgcaga gttccgccgc ttgtgaactt accacgccca gcaatgtgcc
    11101 ggtttctttg acaggagaaa gaatatcggc acgcaggcca gccatatcca gcaatgcagg
    11161 tttccagtca cggatggtta gatcaagaag tcccgtggtg ccagcgttag agggatccac
    11221 cgcaagttcg ccgctgagca tataggccag ccagtcgctg atcatggtga tggttgatgc
    11281 ctgacggtaa atatcggaac gatggtgcgc cagccaaagt aatctgggga tggcacttaa
    11341 agccagtgtt tgtccggtcg cgcgataaac ttcgttttca aaggtattgt tgtgcagttc
    11401 tttaagttcg ctaacttcgc gcgccgctct ggcatccaca ttggcgcagg cccagatcgg
    11461 tgttccttca ttgttatata aaacaatgcc ttcacgcatc gaacatgccg aaacggcagc
    11521 gatatactcc ggggctatgc cggcgttgtg cagcgcctgg cgcatacact cacacgccag
    11581 ttgccagttt ttgttgagat caaattccat agaaccagga acgtccggta ctgccagatg
    11641 ccgccactcc gcctgtccca ctgctatttg attgccttcc aggtcgaata tcacagcccg
    11701 aatacttccg gtgcctgcat ccagcgccat caggtagtac tttgattctg aaggggtaaa
    11761 gagtcgagcc attagattat cctcggctta tgcgttctgt ctgttcctct atacgttctc
    11821 catcattccc ggtaataagg tcatgcaaat ttaactacgt aaaatcgccg ctgctgtgtc
    11881 ctgatcggta accagtgcgt tgatataacc gcctttcatt gcagcggcaa ttgcttcggc
    11941 tttattttct ccccctgcca cgccaacccg gacgggtatg gtcttcagcg cgcttaaagg
    12001 taagccaatc agctcgttat gtattttaat gtccgtgaca acgtcacctt ttgcatcaaa
    12061 aaagtagcct aaaatgtcgc caaccgcccc ttttcggcca atcattaact gttcgccctg
    12121 gctgatataa ccggagcgaa tgatcgtcgc gtcgtcttgt tgactaacgg ccccaatgcc
    12181 gacaatcgcc acatccgctg cttgcgcggc taacagaaca tctttgacac aattttcatt
    12241 ttttagcgta cgggcaatgt cagcggagga tgcccgcaac ggagccggaa taatattcac
    12301 actgcacgcc gcattaagct gtccgatacc cgtcatataa gaaccgacgc caccggagag
    12361 cgtgaccagg cgaatttgct gtgacgaaat aaaaccactt aagcgttgca gcgtattcat
    12421 ggttgcctcg ccaaaaccaa tcgccagcat ctgttgtggt tgaagtaaac tcatcaacat
    12481 atgcgccgcg cctatcccca gtcgcccgcc gacatcagca tccgcaagcc cagggatcac
    12541 ccggacatgt tgcagcgaaa actgacgacg taattgtgtt tcatattcca gacagccttc
    12601 aaagcgagaa ttaatctgca cgcgaatgat gccggactga tgccctttct ccagtaatcg
    12661 cgacactttc aaacgggtca ggccgagacg atcgctgatc tcactctggg tcagcccgtc
    12721 gtggtagtaa aaccacgcga tccgcgcgac ctgttcttct tcacacattc cctgttctga
    12781 aattaccgaa tcgttgattg tcataattca ttcttcactt tgaacatatt taaatcttta
    12841 atgcaattgt tcagttcttg cgcatttata tctgtgatgg caaccacagt ttgactctac
    12901 gagcatgaac aaacgcaacc gtgaaaatca aaatagtata aattgtgatc tattcgtcgg
    12961 aaatatgtgc aatgtccacc taaggttatg aacaaattaa aagcagaaat acatttgttc
    13021 aaaactcacc tgcaaaactg aacgggggaa atatgcaaac gagtgatacc cgcgcgttac
    13081 cgctactttg cgcccgctcg gtttataaac agtattcagg ggtcaatgtc ctgaaaggca
    13141 tcgattttac gttgcatcag ggggaggtcc acgccctgct cggcggcaat ggtgccggta
    13201 aatcgacgtt aatgaagatt attgccggta ttacctctgc tgatagcggt acgctggaga
    13261 ttgggggcaa caactacgcc agattaacgc cagttcatgc tcatcagctg ggtatttatc
    13321 tcgttcccca ggaaccgctg cttttcccaa gcctgtcgat aaaagaaaac atcctgtttg
    13381 ggctggcaaa aaaacagctc tccatgcaga aaatgaagaa cttgctggcg gcgctgggct
    13441 gccagtttga tctgcatagt ctggcaggat cgctggatgt cgccgatcgc caaatggtgg
    13501 aaatcctccg cgggctgatg cgcgactcgc ggattctgat cctcgatgaa cctaccgcct
    13561 cgcttacccc tgcggaaacc gaacgcttgt ttactcgctt gcaagagctg cttgctactg
    13621 gcgtgggtat tgtttttatc tcgcataagc tgccggaaat tcgccagatt gccgatcgga
    13681 ttagcgtgat gcgcgacgga accatcgcct taagcggcaa aaccagcgaa ctgtctaccg
    13741 acgacattat tcaggccatc accccagcgg tacgggaaaa atcgctctct gccagccaaa
    13801 aattatggct ggagttacct ggcaaccgcc cacaacatgc cgcaggaacg tcggtgctga
    13861 cactggaaaa tctgactggc gaaggtttca ggaatgtcag cctgacgcta aatgccggag
    13921 aaattctggg tctggctggg ctggtgggtg ccggacgcac agaactggcc gagacgctct
    13981 atggtctacg tactttgcgt ggcggacgca ttatgctgaa tgataaagag atcaatagat
    14041 tatccactgg agaacgttta ctgcgcggtc tggtttatct gccggaagat cgccagtcat
    14101 ccggactgaa tctcgatgct tcgctggcct ggaacgtctg cgcccttact cataaccttc
    14161 gtggattctg ggcgaaaacc gcgaaagata atgccaccct ggaactttat cgtcgggcgc
    14221 tgaatattaa atataaccaa ccggaacaag ctgcacggac attatccggt ggtaaccagc
    14281 aaaaaatcct gattgccaaa tgcctggaag cctcgccgca agtattgatt gtcgatgagc
    14341 cgacgcgcgg cgtggatgtc tcggcgcgta atgatatcta ccaactgttg cgcagcatcg
    14401 ccgcacagaa tgtggctatg ctgcttatct cctctgatct ggaagagatc gaactgatgg
    14461 cagatcgtgt gtatgtgatg catcagggcg aaattaccca ctctgcactg accgggcgcg
    14521 atattaatgt cgagactatt atgcgcgttg ccttcggcga tagtcagcgt caggaggcgg
    14581 catgctgaag tttattcaga acaaccgtga aatcacggca ctgctggcgg tgctgctgct
    14641 gtttgtatta cccggttttc tcgaccgcca gtatttaagt gtgcaaacgc tgaccatggt
    14701 ttatagcagc gcgcaaatcc tgatcctgct ggcaatgggc gcgacgctgg taatgcttac
    14761 gcgcaatatt gatgtttcag tgggttcgat taccggaatg tgcgcggtgc tgttggggat
    14821 gttactgaac gcaggatatt cactacctgt tgcctgtgtc gcgactttac tgcttggttt
    14881 gctcgcggga tttttcaacg gtgtcctggt cgcgtggcta aagatccctg ccattgttgc
    14941 cacccttggc acgttagggt tgtacagagg catcatgttg ctgtggactg gcggtaaatg
    15001 gattgaaggg ttacccgccg aactgaaaca gctctccgcc ccgctgctgc ttggcgtttc
    15061 agcaattggt tggttaacga taattctggt ggcatttatg gcctggctgc tggcgaagac
    15121 agcgtttgga cgcagttttt atgccacggg cgataattta cagggcgctc gtcaactggg
    15181 cgttcgtacg gaagccattc gcattgtggc attttcgttg aacggctgca tggcggcact
    15241 ggcgggaatt gtgtttgctt cgcagattgg ttttatcccc aaccagaccg gtaccgggct
    15301 ggagatgaaa gcaattgcag cctgcgtgct gggcggcatt agtttgctcg gcggttccgg
    15361 tgcgatcatc ggtgcggtac tcggcgcatg gttcctgacg cagatcgata gcgtactggt
    15421 gctgttgcgc attccggcat ggtggaatga ttttatcgcg ggtctggttc tgctggcggt
    15481 gctggtgttt gatggacgcc tgcgttgtgc gctggaactt aatctgcggc ggcaaaaata
    15541 tgcccgcttt atgacgccac cgccatccgt taaacccgct tcgtcaggta aaaaacggga
    15601 ggctgcataa tgcgtattcg ctacggttgg gaactggctc ttgccgcact gcttgttatt
    15661 gagattgtct catttggtgc aattaacccg cggatgttag atctcaatat gttgctgttc
    15721 agcaccagtg actttatctg cattggcatt gtcgccctac cgctgacgat ggtgattgtc
    15781 agtggcggga tcgatatttc gtttggttcg accatcggcc tctgcgccat tgcattgggc
    15841 gtactgtttc aaagtggtgt gccgatgccg ctggcgattc tcctgacctt actgctcggc
    15901 gcattgtgcg ggctgatcaa cgccggatta attatctata ccaaagttaa cccgctggtg
    15961 attacgcttg gcacgctgta tctgtttgcc ggaagcgccc tgctgctttc cgggatggcc
    16021 ggggcgacgg ggtacgaagg tattggtgga ttcccgatgg cgtttacaga tttcgctaac
    16081 ctggatgtgt tggggctccc cgttccgctg attatcttcc tgatatgtct cctcgttttc
    16141 tggctctggc tgcataaaac ccatgccgga cgtaacgtgt ttttgattgg gcaaagcccg
    16201 cgcgtggcgc tttatagcgc gattccagtt aaccgtacct tatgtgcgct ctatgccatg
    16261 acggggctgg cgtctgcggt cgccgctgtg ctgctggtgt cgtattttgg ttcagcacgt
    16321 tccgatctcg gtgcgtcgtt tctgatgccc gccatcaccg ccgtggtgct tggcggtgcc
    16381 aatatttatg gtggttccgg tgccattatc ggcaccgcca ttgcggtttt attagtggga
    16441 tatttgcaac aaggtttgca aatggcagga gtgccaaatc aggtgtccag cgccctttcc
    16501 ggtgcgctac ttatcgtcgt tgtcgtaggt cgttccgtta gcctgcatcg ccagcaaatt
    16561 aaagagtggc tggcgcgtcg ggccaataac ccattgccat aaaggatatc ttcatgacac
    16621 ttcatcgctt taagaaaatc gccttactta gcgctcttgg cattgccgca atctctatga
    16681 atgtgcaggc cgcagagcgt attgcattta ttcccaaact ggttggcgtg ggatttttta
    16741 ccagcggtgg caacggcgca caacaagcgg gtaaagagct gggcgttgat gtgacctacg
    16801 acgggccgac agaacccagt gtttctggtc aggtacagtt gattaataac ttcgtcaatc
    16861 aaggttataa cgccattatc gtttctgcgg tttcgcctga tggcttgtgt ccggcactga
    16921 aacgcgccat gcaacgtggt gtgagagtgc tgacctggga ctctgatact aaaccggagt
    16981 gccgctctta ctacattaat cagggaactc ccgcccagtt gggaggtatg ttggtggata
    17041 tggcggcgcg tcaggtgaat aaagacaaag ccaaagtcgc gtttttctac tcaagcccca
    17101 ccgttacgga ccaaaaccag tgggtgaaag aagcgaaagc gaaaatcgcc aaagagcatc
    17161 ctggctggga aattgtcact acgcagtttg gctataacga tgccactaaa tcattacaaa
    17221 ccgcagaagg aatattaaaa gcgtatagcg atctcgacgc cattatcgcc cccgatgcca
    17281 atgccctgcc cgctgccgca caagccgcag aaaacctgaa aaatgacaaa gttgcgattg
    17341 tcggattcag tacgccaaac gtgatgcgtc catatgtgga acgcggcacg gtgaaagaat
    17401 ttggcctgtg ggatgtggtt cagcaaggca aaatttcagt ttatgtcgcg gatgcattat
    17461 tgaaaaaagg atcaatgaaa acgggcgaca agctggatat tcagggcgta ggtcaggtgg
    17521 aagtctcgcc aaacagcgtc cagggctatg actacgaagc ggatggtaat ggcatcgtac
    17581 tgttaccgga gcgcgtgata ttcaacaaag agaatatcgg caaatacgat ttctgatgtg
    17641 cattacttaa ccggagtaag ttatggcaga tttagacgat attaaagatg gtaaagattt
    17701 tcgtaccgat caaccgcaaa aaaatatccc ttttaccctg aaaggttgcg gtgcgctgga
    17761 ttggggaatg cagtcacgct tatcgcggat atttaatccg aaaacgggta aaaccgtgat
    17821 gctggctttt gaccatggtt attttcaggg accaactacc ggacttgaac gcattgatat
    17881 aaatatcgcc ccgctgtttg aacatgccga tgtattaatg tgtacgcgcg ggattttgcg
    17941 cagcgtagtt cccccggcga ccaataagcc ggtggtactg cgggcgtcag gtgcgaactc
    18001 tatcctggcg gaattaagca atgaagccgt ggcgttatcg atggatgacg ccgtgcgcct
    18061 gaacagttgc gcggtggcgg cgcaggttta tatcggcagc gaatatgaac atcagtcgat
    18121 caaaaatatt attcagctgg tcgatgccgg aatgaaagtg ggaatgccga ccatggccgt
    18181 gactggcgtg ggcaaagaca tggtgcgcga tcagcgttat ttctcgctcg cgactcgaat
    18241 cgccgctgaa atggggacgc aaattatcaa aacctattat gtcgaaaaag gttttgaacg
    18301 gattgttgcc ggatgtccgg tacccattgt tattgctggc ggtaaaaaat taccggagcg
    18361 cgaggcgctg gaaatgtgct ggcaggctat cgatcagggc gcttctggtg tggatatggg
    18421 gcgtaatatt ttccagtctg accatccggt ggcgatgatg aaagccgtac aggcggtggt
    18481 tcaccataac gaaacggctg atcgggcata tgaactctat ctgagtgaaa aacagtaact
    18541 gcggatttaa ggagaagaat tatgcacgtc acactggttg aaattaacgt tcatgaagac
    18601 aaggttgacg agtttatcga agtttttcgc cagaaccacc tgggctctgt acaggaagaa
    18661 ggcaatttgc gcttcgatgt cttacaggac ccggaagtga attcgcgctt ttatatctac
    18721 gaagcctata aagatgaaga cgcagtggcg ttccataaaa ccacgcccca ctacaaaacc
    18781 tgtgtcgcga aactggaatc tttaatgacc gggccgcgta aaaaacgtct gttcaatggg
    18841 ttgatgccgt gaggcgaatt tatcagtttt atctacaatt ggggtaacgc gctgacggga
    18901 gtaaataaat gtctgactgg aacccctctt tatatctgca ctttgccgct gaacgatcgc
    18961 gtccggcggt ggagctgctt gccagagtgc cgctggagaa tgtcgattat gttgccgatc
    19021 ttggctgtgg cccaggtaac agcaccgccc tgctgcatca acgttggcct gctgccagga
    19081 taacgggcat cgactcgtct ccggcgatga ttgctgaagc gcgcagtgct ttgccagact
    19141 gccagttcgt ggaggcagac atccgcaact ggtaagcgcg aggatatctt ggataatact
    19201 gagccatgcc agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt
    19261 atcttcaggc gtggaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacac
    19321 tacttacaaa ttacctacaa aattacaaca ttaacaggtg ctgcatgtgg cacctgttaa
    19381 tgataaaggc ttataggatg ttgtaactaa tataatgaaa taagaaaccc ggtcgaaacc
    19441 gggttcagag tattagcaca acggacgtac agcttcgcgc atcccttttt cgagaatcgc
    19501 atccaggtca ttagccacct gctcgaccag accaggcact tgcgtcaggt cctgctccca
    19561 gtggtctttt tctgccaaca caatcgctac cagttcctgg gtaccgataa cacggtcacg
    19621 atgctgactc cagagttgct gataacgttc cagccagtgt gcatcgtcct gtaccggata
    19681 tgtttctcca ttacgctcac cgcgatagaa cgcaatcaat gccgctaatg cgaaagtaag
    19741 gcgcgccgga agtgtgccgt ttgccttctg ccctgccagc agctgcggca ggatgcgggt
    19801 acggaacttg gtcataccgt tgagcgcgat agacagcagc tgatgcttaa tgtacgggtt
    19861 acggaaacgc ccggtgactg cactggcgaa agattccagt tcatcgcgtg gcaaatccag
    19921 taccggaata atttcttcgt agatggcttt ttcaacgaat gcgcaaattt cagcatcgtt
    19981 catcgcttca cctacggtat ccagccctgc ctggaacgcc accggcacca acgcggtgtg
    20041 tgcaccgttg agtattgcca ctttgcgttc tttatacggt ttaatatcgt caacaatcag
    20101 cacgttgagc ggatatttgt ccagacgcag ctcggtcgct aaggatttcg gtccctgaat
    20161 cacaaacaag taaaagtgtt cagcggtgtc gaggaaacca tcgtgataac ccagttcttc
    20221 ttccagttta gccacttcat cacgcggata accggtaacg atacggtcca ccagcgtaga
    20281 acagaagctg ttagcctgat ccagccattg aataaatgct tctggcagtg cccactcttg
    20341 cgcatagcgc agcaccagtt cacgcaacgc gtcgccattg tagtcgatca actcacacgg
    20401 gatgatgatc caacctttat ccagcgcacc gttgaaatgg ctaaaacgtt cgaacagcag
    20461 acgggtcagt tttgccggat aacttaccgc tggcgcgtca tcgaatttat cgcccgcatg
    20521 gtagctgata cccgcttcgg tggtgttaga gaaaacaaag cgcatttccg ggttgtgcgc
    20581 cagtttcagg aattcatcgt attcactgta gacgctgatt tcacgattaa cagagcgaat
    20641 cagacgcgcg tcgctgaccg cttccccctt ctcattcagg ccacggataa tggtggtgta
    20701 cagaccatcc tgcgtgctca gtgacggcgg gaatgaagtt tcaatcggac gaacaacgac
    20761 cacaccagaa ttcagatcgg tgtgctcatt caggagatct atttgccagt caacaaaggc
    20821 gcgcaggaag ttaccttcac caaactgaat gatacgttct ggatactgtg caccgggaaa
    20881 atcgcgacga tttagtgttt tcacaatggg ttcccttctg attagtcata caacctgttt
    20941 gaattggtac gacaggttag caaactttaa tacgacgaac ccctgttttg atcaactcct
    21001 gatgattaat gagcagtttt atgagaaaag tgtggcgcgg atcatggttt aatcgaggaa
    21061 atacgccttt tcctggatca taaagtggta gaacacattg cattcaaatc gcgcggaatg
    21121 aataaagatg tcagacaact tcctcaccgt aacgcatagt gctggtacgg ttgcgcccat
    21181 ctttcttcga gcgatacaga caggtatcgg cttcgaccat caatttatta aagtcatcgg
    21241 tcagcgttcg gtacgatgcg cgaccactac cgacgccaat acttaccgtg agataaagcg
    21301 ttttttgttg ccaggtgaat ggttgcagtt caacgccttt acggattttt tccgccatta
    21361 gcagaccatc gacaggattc actgacggca ctgcaacagc aaattcttcg ccgcccatgc
    21421 gcgccaccag ccccttatca ccgacaatct tctgaatatg ccgggcaaac acgcttaaca
    21481 ctttatcgcc acattcatgt ccatagttat cgttaatact tttgaaatag tcgatatcaa
    21541 gtagcatgac agtcagatgt tgtgtctgtt tcagcgatgg acttttcagc gcctcataaa
    21601 gaccggaccg tgagtaaacc tgagtcagaa aatcaaagtc ggctcgcagc gcaacttgct
    21661 tcattagcga attgatcgct gccacgctaa aagaaaccat aattgggcat atcgccatcg
    21721 tggcaatacc gagacgtgcg gagaacattt gcggaatgga gaacggcgaa ccgaccgaga
    21781 tatcaatcac cgaattagcc accagtacga tttccaccgc accggtgaca aaggtcaaca
    21841 gacatgttac ctgcggcgta tagcgcactg cacaccagat taatgcaggc agcgggaacg
    21901 ccagactccc cgctccgcca atgactaccg aggcaataac cgacacaata agcgcgatgg
    21961 cgggcatcat ctgttctgct ttaaagcgcg gcagcactcc aggaatagcc aacgtcagca
    22021 tacaaggcac gatcaacacg cccgttgaga attgctcact gaaccagtcc gcaagcaaag
    22081 gccagaaatc cagactatca atactgaccg aaccaatcgc cccgacaata gcgcataata
    22141 atgcggcaat cagacagtaa ttaaatagcc gtaaggcact tactggctca tatttatttt
    22201 tccccaaacg cttatcccgc acgaccagta aggcgacggt aacaataaac atcatattgg
    22261 agaaattgat agccagtgaa accagtcccc attcggtggt tatggcatca taaaccagca
    22321 tcgccacata actgatcgca tagtaatgca gtcgattaag ccagacatag cgggcaaaca
    22381 cccccgccat tacgccattc agcggccaga aaagagaaag ttcgtgaacc agccgtaact
    22441 ctgcaccaat aaaatagaac agcgtggtta gcgcaaaaat agcgatgcta ttacgtagca
    22501 gatgaccttc ctggaacaca cggaaagttg aaatggggtg tacatgcatt aattatctgt
    22561 ccatacaatt gcgggtggca attatctcaa cgtaataagt aaattctcat aaaggatagc
    22621 atgttcgctg acagcggcgg caaataaagg taccctggga atgctatttt gtcgttttcc
    22681 gcaattctca tggctgattc atctgtacaa ccagccagtg atagattacc gccacgatat
    22741 aacgttgttg ttcttcgctt agcgaaacac catgcgggat gttatgccac ttagccagac
    22801 aaccgcgaca gcaggtcgcg gtggcgtgct gagcgataaa caccggatgc ccgcgcatgg
    22861 gggtttgctt accgtcatta gccggtaatg ccggagcaag acgtttagcg acaaaatccg
    22921 ccgcatgttg ttcgatgact ggcgcgcctt tctccagaca atactgacgc tctttcatgc
    22981 ccaaacgaaa gcgagatcga aattttgaac gggataaacg cgcgaagagt ggatcgagag
    23041 actgcattaa taaaccgaac gccctaactg tttcgtcaat tgttcaagga cggcgatacc
    23101 cgcaagcgag ttacctgcat catccagttc cggactccag actgcgatgg ccatttcatg
    23161 cggaacaatc gccacaatac cgccaccaac gccagatttc gccggtagtc ccacccgcca
    23221 ggcaaactcc cccgcgttct ggtacatacc actggtcgcc atcagcgcgt taatttgccg
    23281 tgcctgcatt ggcgtcacca caggttcatc aatatgaata gctttcccct gattagccag
    23341 aaagacaaac gtccgggcca gctctacaca gctcattttc agagcgcagt aatgaaaata
    23401 gttttgcaga acggttgtca cgtcatgatg gaaattgcca aacgacttca tcagccaggc
    23461 gatagccgca tttcgcgcgg aatgttcaaa ttcggaacgc gctaccaccg tatcgtagga
    23521 aatatcagac acaccgctta agccgcgcac gacttccagc atacgttgcc gtggtgcgct
    23581 taatcgccct tgcaacatat cgcagaccac cagcgcacca gcattaataa acggattacg
    23641 cggaataccc tgctccattt ccagttgcac taaagaattg aacggtgatc cagacggatc
    23701 tttgccgacg cgttgccaga tttcctcttc ggagtaatga cgcatggcga caacgagact
    23761 cagcactttg gaaatagact gaatggaaaa acgttcttgc gcgtctccgg cctgaaaaag
    23821 ctgtccgtca acggtacaga tagcaatgcc caatcgggaa ccgtctactg tagccagcgc
    23881 cggaatataa tccgcgactt taccctgacc aatgagcggc cgcacttgcc gcaagatgtt
    23941 ttctaaaatt gcattatcca tggcgactgc cactttctac tcctggaccg caggtctgaa
    24001 aagacctgcg agtatatcag agctgaatat gtcgcgtcag atccggtctt tccacaccgt
    24061 ctggatatta cagaattcgt gtaagccgaa atgggaaagc tcacgaccaa agccactctt
    24121 tttcacgcca ccaaaggcca ctcgcgcgtc gctggcacaa taaccattga taaacacccc
    24181 accgcattcc agacgtgccg ccatctgtct ggcctgtgtt tcgtcggtgg taaaaatggt
    24241 cgctgaaagg ccgaactcac tatcattagc cagttccagc gcatgttctg catctttcgc
    24301 aatggtgatt gccgcaacgg ggccaaacat ttcttcccga aaagcggtca tttctggggt
    24361 aacattcgcc agaaccgttg gcggatagta gttacctgcc ccagccatct tttccccgcc
    24421 cagtaacaaa cgcgcaccct gcgccagggt tttctccacc tgatgatgca gctcatcacg
    24481 taaatcgaaa cgagccattg gtccgagagc gttctcttcg tcacggggat cgtccatttt
    24541 caaggctgcc gcagctgcca caaaacgttc ggtaaatgcc gaagcaattc cctcttcgat
    24601 aataaagcgt tttgccgctg cacaaacctg tccggtattc tgataacgtc cggctaccgc
    24661 cgctttaact gccagttcca gatcggcatc gttaagcaca ataaatggat ccgaaccgcc
    24721 cagttccagt acgcattttt tcagtgccgc tccagcctgt gcaccaatag ccgctccagc
    24781 acgaacactt ccggtcaccg tgatagcagc aatgcgcgag tctttaatca tctgagtgac
    24841 accgtcattg tcggcattca gccagccata tacgccttgt gggattcccg catctttaaa
    24901 cacctgggca atgagctgag cacagcccat cacattcggc gcatgtttaa gtaagtatcc
    24961 gttaccagca agaatgatgg gaacagcgcc acgcatcacc tgccataacg gaaaattcca
    25021 cggcataatc gccagaatcg tccccaacgg tcgatactca ataactgcct gctgattttc
    25081 caccagcgta ggttccgcct tcagcattgc cggaccatgt tctgcatacc agtcacacaa
    25141 attcgccgat ttcgccactt cagcgcgcgc ctggttgatt ggtttgccca tttcgcgggt
    25201 gatcatttgc gccatttctt cgctacgggc gcgcagagcc ttaccgatat cacgcagttt
    25261 ttcagcacga taatctatat ttgtctcgcg ccagtcacga aagcctgctg ccgccagctg
    25321 aagtgcgttt tcgatatcat cagcgccagc ccacggcaac acagaaagtt gttcacccgt
    25381 ggcaggattt atcgaaattg catgagttgc cggagtaatg gtcatcgggg tatctccttt
    25441 atgagtcatg gtatgaagat acgcagattt actcttgctt taaaatgaat aatattaagc
    25501 cacttattca cgaatcgaga atattatgga tctgacccaa ctggagatgt tcaacgcggt
    25561 tgccgaagct ggcagcatta cgcaggctgc agcaatagtg catcgcgtgc cgtcgaatct
    25621 cactacccgt ctacgccagc tggaaacaga actgggggta gatctgttta ttcgcgagaa
    25681 tcagcgttta cgtctatctc ctgccgggca taacttttta cgctatagcc aacaaattct
    25741 cgcgttagtg gatgaagcgc ggagcgttgt cgctggcgat gagccgcaag gtttattttc
    25801 tctgggttcg ctggaaagca ccgcagcagt tcgcattccg gccacgctgg cggaattcaa
    25861 ccgtcgttat cccaaaattc agttttcact ttccaccggc ccttccggca ctatgctgga
    25921 aggtgtgctg gagggaaaac ttaatgcggc gtttattgat ggacccatta accatactgc
    25981 cattgacggg ataccggtat accgcgagga gctgatgatc gtcacgccac aaggtcatgc
    26041 gccagtaatc cgtgccagtc aggttaatgg cagtaacatt tatgccttcc gcgccaattg
    26101 ttcgtatcgt cgccacttcg agagctggtt tcatgctgac ggtgccgctc cgggaactat
    26161 ccatgagatg gagtcttatc acggaatgtt ggcctgtgtg gtcgcaggag caggtattgc
    26221 gcttattccg cgctctatgc tggaaagtat gccggggcat caccaggttg aagcgtggcc
    26281 gttagctgag caatggtgtt ggctaacaac ctggctggtc tggcgtcgtg gtgcgaaaac
    26341 gcgtccgctc gaggcattta ttcaactact ggatgtgcct gactcgacaa aacagggtta
    26401 ccaatgagct atttttgata gttcttgcgg ctaatatgct ctatatagtg atgttccgat
    26461 gacttatgac tatatggggc aaatatggtt acgccagtaa gcatcagcaa ttacatatct
    26521 cttcctgatg attttcccgt tcgtaatatt gcaccgcagg taaaagaagt tttaaaggat
    26581 tttattgatg cacttagtac aataatatgt aatgaagagt ggcgcacgtc tttaaacatc
    26641 aactcagcca caaaaaagat atttaataat cttgacaact tatcatatat tcagaggact
    26701 tcttttcggg gtaacgacac gctatacaat gaaaaggttc agtttaaact cacttatccc
    26761 gccaggaatg gaagacacaa agaaaatatt gaatttcagg tagtaataaa tttaagccct
    26821 atttatttag ataatttccg ccatgatgga gaaattaata ttttttgcgc tcccaaccca
    26881 aagcctgtta ctatggggcg cgtatttcag accggcgtcg agcgtgtgct ctttctgttt
    26941 ctgaatgatt ttattgaaca attcccaatg atcaaccctg gcgttcccat caaaagagcg
    27001 catacaccac atattgaacc cctgcctccg gatcaccata ccgctgcaga ttacttacgc
    27061 cagtttgatt tgcttgtcct gaattttatc tctcgcggta attttgtcat actcccccga
    27121 ttatggaata actctgaggt tcacagatgg ttcgtcaata aagatcctaa cttgatcacc
    27181 gccattctcg acataacgga cagcgaatta aaagaggatt tattgcaaag cctgatggat
    27241 tcattaggtt ctaacaaaca tgtactaccc taagtctgca tctgtttttt atccctttta
    27301 gcagaacaag aatctcctca ttttcaggac ttgtttttat ttttcgccaa tatgttgtta
    27361 cactatcacc aatttatgaa tcccaatgaa agtgatttga atgacgtgtt gatgccagca
    27421 tcattaagtg atgataaaat tatcaaacat atggcgcgta agaccctcaa actgtttgta
    27481 aaaaatgaaa caccgccaaa agttactcat gaagatctgg tgaaaaacag gcctcgctcc
    27541 cctgtcagac cgcctatacc cgcaacctcc aaaacgccag acattcctga tcgtcattaa
    27601 accgtgatgt ttaccatctc tctgacgcgt gaaagaatca gcgtcagaga aacggaaaac
    27661 gcgatccaga tcacaaatgc attgtattca catcattaac cgttttaaga tcatttcatc
    27721 actttttcgc aactcacccg ataatctgtt atgacaacaa acactgtttc ccgcaaagtg
    27781 gcgtggctac gggtcgttac gctggcagtc gccgccttca tcttcaacac caccgaattt
    27841 gtccctgttg gcctgctctc tgacattgcg caaagttttc acatgcaaac cgctcaggtc
    27901 ggcatcatgt tgaccattta cgcatgggta gtagcgctaa tgtcattgcc ttttatgtta
    27961 atgaccagcc aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc
    28021 agccacgtac tgtcgttttt atcgtggagc tttaccgttc tggtgatcag tcgcattggt
    28081 gtggcttttg cacatgcgat tttctggtcg attacggcgt ccctggcgat ccgtatggct
    28141 ccggccggga agcgagcaca ggcattgagt ttaattgcca caggtacagc actggcaatg
    28201 gtattaggat tacctctcgg gcgcattgtg gggcagtatt tcggttggcg aatgaccttc
    28261 ttcgcgattg gtatcggggc gcttattacc cttttgtgcc tgattaagtt acttccctta
    28321 ctgcccagtg agcattccgg ttcattgaaa agcctcccgc tattattccg ccgcccggca
    28381 ttgatgagca tttatttgtt aactgtggtg gttgtcaccg cccattacac ggcatacagc
    28441 tatattgagc cttttgtgca aaacattgcg ggattcagcg ccaactttgc cacggcatta
    28501 ctgttattac tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa actgggtaat
    28561 caatatgcgt ctgcgttggt aagtacggcg attgcgctgt tgctggtgtg cctggcactg
    28621 ctgctacctg cggcgaacag tgaaatacac ctcggggtgc tgagtatttt ctgggggatc
    28681 gcgatgatga tcatcgggct tggtatgcag gttaaagtgc tggcgctggc accagatgcc
    28741 accgacgtcg cgatggcgct attctccggg atatttaata ttggaatcgg ggcgggtgcg
    28801 ttggtaggta atcaggtgag tctgcactgg tcaatgtcga tgattggtta tgtgggcacg
    28861 gtgcctgctt ttgccgcgtt aatatggtca atcattatat ttcgccgctg gccagtgaca
    28921 ctcgaagaac agacgcaata gttgaaaggc ccattcgggc cttttttaat gattcgtttt
    28981 aatgatttcc aggatgccgt taataataaa ctgtacaccc atacatacca gcaggaaccc
    29041 catcaagcgg gagatcgctt caatgccacc cttgcccacc agccgcataa ttgcgccaga
    29101 gctgcgtaga cttccccaca aaataaccgc caccaggaaa aagatcagcg gcggcgcaac
    29161 catcagtacc caatcagcga aggttgaact ctgacgcact gtggacgccg agctaataat
    29221 catcgcaatg gttcccggac cggcggtact tggcattgcc agtggtacaa aagcaatatt
    29281 ggcgctgggt tcatcttcca gctcttccga cttgcttttc gcctccggtg aatcaatcgc
    29341 tttctgttgc ggaaaaagca tccgaaaacc gataaacgcg acgattaagc cgcctgcaat
    29401 tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc agttgcccgg cgtaatacgc
    29461 caccatcatg atggcaaaga cgtacaccga ggccatcaac gactgacgat tacgttcggc
    29521 accgttcatg ttgcctgcca ggccaagaaa taacgcgaca gttgttaatg ggttagctaa
    29581 cggcagtaac accaccagcc ccaggccaat tgctttaaac aaatctaaca ttggtggttg
    29641 ttatcctgtg tatctgggtt atcagcgaaa agtataaggg gtaaacaagg ataaagtgtc
    29701 actctttagc taaccttgca tcgcattgac caaaacttga accgatttag caaaacgtgg
    29761 catcggtcaa ttcattcatt tgacttatac ttgcctgggc aatattatcc cctgcaacta
    29821 attacttgcc agggcaacta atgtgaaaag taccagcgat ctgttcaatg aaattattcc
    29881 attgggtcgc ttaatccata tggttaatca gaagaaagat cgtctgctta acgagtatct
    29941 gtctccgctg gatattaccg cggcacagtt taaggtgctc tgctctatcc gctgcgcggc
    30001 gtgtattact ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag cactgacccg
    30061 tatgctggat cgcctggtct gtaaaggctg ggtagaaagg ttgccgaacc cgaatgataa
    30121 gcgcggcgta ctggtaaaac ttaccaccag cggcgcggca atatgtgaac aatgccatca
    30181 attagttggc cagaacctgc atcaagaatt aacaaaaaac ctgacggcgg acgaagtggc
    30241 aacacttgag catttgctta agaaagtcct gccgtaaaca aaaaagaggt atgacgatgt
    30301 ccagacgcaa tactgacgct attaccattc atagcatttt ggactggatc gaggacaacc
    30361 tggaatcacc actgtcactg gagaaagtgt cagagcgttc gggttactct aaatggcacc
    30421 tgcaacggat gtttaaaaaa gaaaccggtc attcattagg ccaatacatc cgcagccgta
    30481 agatgacgga aatcgcgcaa aagctgaagg aaagtaacga gccgatactc tatctggcag
    30541 aacgatatgg cttcgagtcg caacaaactc tgacccgaac cttcaaaaat tactttgatg
    30601 ttccgccgca taaataccgg atgaccaata tgcagggcga atcgcgcttt ttacatccat
    30661 taaaccatta caacagctag ttgaaaacgt gacaacgtca ctgaggcaat tatgaaaccg
    30721 cttttatccg caatagcagc tgcgcttatt ctcttttccg cgcagggcgt tgcggaacaa
    30781 acccagcaac cgctcgttac ttcctgtggc gatgtggtgg ttgttccccc atcgcaggaa
    30841 caaccaccgt tcgatttaaa tcacatgggt acaggcagtg acaaatcgga tgcgctgggc
    30901 gtgccctatt ataaccaaca agccatgtag tatattcagg ccccggcata tcggggcttg
    30961 ttaacctctt accttcaccg ctttacgcca ctgcaaacca aatacattga tatacagtcc
    31021 ggccataatg agcacagccc ctaagaattg cagacctgtt aagcgttcat ccagcagtaa
    31081 cgctgcactt gccagaccga ctaccggcac cagtaatgat aatggtgcga cacgccatgt
    31141 ttcatagcgt ccaagcagcg taccccaaat cccgtagcca actatcgtcg ctacaaatgc
    31201 cagatacatc aacgccagaa tggtggtcat gtcgatagtg accagactgt gaatcatttt
    31261 tccggagcca tcgagaatga gcgaggcgac aaaaaacgga attatcggga ttaatgcgct
    31321 ccagacgacc agagacatta ccgccggacg ggatgaatga gacatgatct ttttgttgaa
    31381 gatattgcca ctcgcccaac taaatgccgc tgccagagtc aacataaagc cgagcatcgc
    31441 cacatgctga ccgttaaagc tatcttcgat taacaccagt acgccagaaa tcgctaacgc
    31501 aatccccgcc agttgcttac cgtgcagccg ttcgccaaaa gtaaacgcgc caagcacgat
    31561 agtaaaaaac gcctgagcct gtaagaccag cgaggccagt ccagccggca taccgaagtt
    31621 aatggcgcag aagagaaagg caaactgtgc aaaactgatg gttaaaccat accccagcag
    31681 taaattcagc ggtacttttg gtcgtgccac gaaaaagatt gccgggaagg cgaccagcat
    31741 aaagcgcaaa ccggccagca tcagcggtgg catgttatga agccccactt tgatgaccac
    31801 aaaattaagc ccccatacaa cgacgactag tagcgccaac accccatctt ttcgcgacat
    31861 tctaccgcct ctgaatttca tcttttgtaa gcaatcaact tagctgaatt tacttttctt
    31921 taacagttga ttcgttagtc gccggttacg acggcattaa tgcgcaaata agtcgctata
    31981 cttcggattt ttgccatgct atttctttac atctctaaaa caaaacataa cgaaacgcac
    32041 tgccggacag acaaatgaac ttatccctac gacgctctac cagcgccctt cttgcctcgt
    32101 cgttgttatt aaccatcgga cgcggcgcca cgctgccatt tatgaccatt tacttgagtc
    32161 gccagtacag cctgagtgtc gatctaatcg gttatgcgat gacaattgcg ctcactattg
    32221 gcgtcgtttt tagcctcggt tttggtatcc tggcggataa gttcgacaag aaacgctata
    32281 tgttactggc aattaccgcc ttcgccagcg gttttattgc cattccttta gtgaataacg
    32341 tgacgctggt ggtgctcttt tttgccctca ttaactgcgc ctattccgtt tttgctaccg
    32401 tgctgaaagc ctggtttgcc gacaatcttt catccaccag caaaacgaag atcttctcga
    32461 tcaactacac catgctcaat attggctgga ccgtcggccc gccactcggc acgctgttgg
    32521 tcatgcagag catcaatctg cccttttggc tggccgctat ctgctctgcg tttcccatgt
    32581 ttttcattca aatttgggta aagcgcagcg agaaaatcat cgccacggaa acaggcagtg
    32641 tctggtcgcc gaaagtttta ttacaagata aggcactgtt gtggtttacc tgctctggct
    32701 ttctggcttc ttttgttagt ggcgcatttg cttcatgcat ttcacaatat gtgatggtga
    32761 ttgctgatgg tgattttgcc gaaaaggtgg tcgcggttgt tcttccagtg aatgctgcca
    32821 tggtggttac gttgcaatat tccgtgggcc gccgccttaa cccggctaac atccgcgcgc
    32881 tgatgacagc aggcaccctc tgtttcgtca tcggtctgat cggttttatt ttttccggca
    32941 acagcctgct attgtggggt atgtcagctg cggtatttac tgtcggtgaa ataatttatg
    33001 cgccgggcga gtatatgttg attgaccata ttgcgccgcc aggaatgaaa gccagctatt
    33061 tttccgccca gtcattaggc tggttgggcg cagcggtaaa tccattagtg agtggtgttg
    33121 tacttaccac tctcccgtcg tcttcgctgt ttatcattct ggcgttagtc atcattgccg
    33181 cgtgggcgct gatgttaaaa ggtattaacg ccagacccca gaggcaacca gcactctgtt
    33241 gacgttcgtg ccgttccact actggcacgg cttcgctaat cattattctg aggcaaaggc
    33301 atagccttca tcttcccagc cggttatccc gccgagcatg attttaaccg gctttccgag
    33361 tcgagccagt ttgagtgccg cacggtctgc accgttacaa tgtggccccg cgcaatacac
    33421 cacaaatagc gtatcgtcgg gccattcggc cattctttct gcggtcattt gcttatgggg
    33481 taaatggaga gcgcctggga tatgccgacg cgcgaagtcc tcttcacgtc caaccacatg
    33541 aagtaaaacg aaatcatcgc caatataagc cagagagtca tgaacatcgg cgcaatctgt
    33601 ttcgacactc aagcgcgcga gaaaatgcgc ctcagcaact tctggcttcg ctgtggtaaa
    33661 ttcagtgaca taacgcatat tgctttgtcc ttttggtttg ttgtgttaac aataatgtaa
    33721 ctaatctacc tttcactgac cgcctctcga tatgccagaa atgagcaaaa tgatgacaac
    33781 gttacaacgt ccgctggtca ttgcactggc atatgacggg ctatgtactt ttgaatttgg
    33841 cgttgccgtt gaaatattcg gcctgccacg accggaactg ggcgataact ggtatcgttt
    33901 tgctgttgct gcggtagaac caggtgcgct acaagccacc ggggggatcc acgtggttgc
    33961 tgatggtgga ctggagttat tgcccaacgc cgatatgatt attattcctg gctggcgcgg
    34021 aatagatgcc aaagtgccgg agatgctatg ccagtcattg cgcgaggcac accagcgcgg
    34081 tactcgcctg gtatcaatat gctctggcgt gtttgtcctt gctgctgcgg gccttcttca
    34141 tcagcgaaaa gcaaccaccc actggcgcta cactgaacaa ctccagcacc gttacccttc
    34201 aatcagcgta agtgacaatg tgttatatca ggatgaagga caagtgatga cctccgccgg
    34261 aagcgccgcg gggatcgatc tgtgtttaca tatcgtgcgc aaagattttg gtcatgagat
    34321 tgctaataat gtcgcccggc gactggtcat tcagccacat cgccaggggg atcaaccaca
    34381 aaatctgacg cgcccaatgg cctcaccgcg tgaaagccag acgttaggtg ccttattcga
    34441 ctttttgcag cagaatctgg cacaaacaca tactgtgtct tccctcgccg aacgggtaaa
    34501 tatgagtccg cgcacttttt taagacgttt tgaggcgtta actggcatga cgcccgcccg
    34561 ctggctgctc aatgagcgct taatacgagt acaggattat ctgcaaaatt cggtttttaa
    34621 catcgagaaa attgccgagc tgacgggttt tggcaattca gcgttattgc gtcatcactt
    34681 tcaccaacgt tttttattat ctccttcgca atatcgcaaa aacagccgtt ccccatcaca
    34741 ataatcgcct gattcagcct ttgtttagat aacctctgct aatatctttc tcattgagat
    34801 aaaaattaag gtaagcgagg aaacacacca caccataaac ggaggcaaat aatgctgggt
    34861 aatatgaatg tttttatggc catactggga ataattttat tttctggttt tctggccgcg
    34921 tatttcagcc acaaatggga tgactaatga acggagataa tccctcacct aaccggcccc
    34981 ttgttacagt tgtgtacaag gggcctgatt tttatgacgg cgaaaaaaaa ccgccagtaa
    35041 accggcggtg aatgcttgca tggatagatt tgtgttttgc ttttacgcta acaggcattt
    35101 tcctgcactg ataacggatc gttgacacag tagcatcagt tttctcaatg aatgttaaac
    35161 ggagcttaaa ctcggttaat cacattttgt tcgtcaataa acatgcagcg atttcttccg
    35221 gtttgcttac cctcatacat tgcccggtct gctcttccaa tgaccacatc cagagtctct
    35281 tcaggaaatg cgcgactcac acctgctgtc acggtaatgt tgatatgccc ttcagaatgt
    35341 gtgatggcat ggttatcgac taactggcaa attctgacac ctgcacgaca tgcttcttca
    35401 tcattagccg ctttgacaat aatgataaat tcttcgcccc cgtagcgata aaccgtttcg
    35461 taatcacgcg tccaactggc taagtaagtt gccagggtgc gtaatactac atcgccgatt
    35521 aaatgcccgt aggtatcatt aaccaattta aatcggtcaa tatccaacaa cattaaataa
    35581 agattcagag gctcagtgtt gcgtaactga tgatcaaagg attcatcaag aacccgacgc
    35641 cccggcaatc ccgtcaaaac atccatattg ctacggatcg tcagcaaata aattttgtaa
    35701 tcggttaatg ccgcagtaaa agaaagcacc ccctcctgaa aggcgtcgaa atgcgcgtcc
    35761 tgccagtgat tttcaacaat agccagcatt aattcccgac cacaattatg catatgttga
    35821 tgggcagaat ccattagccg aacgtaaggt aattcatcgt tatcgagtgg cctcagatga
    35881 tcaatccacc gaccaaactg gcacagtcca taagaatggt tatccgttat ttctggctta
    35941 ctggcatctc tcgcgaccac gctgtgaaac atactcacca gccactgata gtgggcatcg
    36001 atagccttat tgagatttaa caagatggca tcaatttccg ttgtcttctt gatcattgcc
    36061 actccttttt cacagttcct tgtgcgcgct attctaacga gagaaaagca aaattacgtc
    36121 aatattttca tagaaatccg aagttatgat tcatctctga gataacattg tgatttaaaa
    36181 caaaatcagc ggataaaa
//
LOCUS       DAZFBR010000038        35866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFBR010000038 DAZFBR010000000
VERSION     DAZFBR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.038"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            46..477
                     /gene="tnpA"
                     /locus_tag="ACHJZ2_002104"
     CDS             46..477
                     /gene="tnpA"
                     /locus_tag="ACHJZ2_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HFO4568212.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            complement(535..828)
                     /locus_tag="ACHJZ2_002105"
                     /pseudo
     CDS             complement(535..828)
                     /locus_tag="ACHJZ2_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            1100..1894
                     /gene="yjfZ"
                     /locus_tag="ACHJZ2_002106"
     CDS             1100..1894
                     /gene="yjfZ"
                     /locus_tag="ACHJZ2_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO4568213.1"
                     /translation="MTLPTTIYSFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            complement(1965..2414)
                     /gene="rplI"
                     /locus_tag="ACHJZ2_002107"
     CDS             complement(1965..2414)
                     /gene="rplI"
                     /locus_tag="ACHJZ2_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO4568214.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(2456..2683)
                     /gene="rpsR"
                     /locus_tag="ACHJZ2_002108"
     CDS             complement(2456..2683)
                     /gene="rpsR"
                     /locus_tag="ACHJZ2_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO4568215.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(2688..3002)
                     /gene="priB"
                     /locus_tag="ACHJZ2_002109"
     CDS             complement(2688..3002)
                     /gene="priB"
                     /locus_tag="ACHJZ2_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO4568216.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(3009..3404)
                     /gene="rpsF"
                     /locus_tag="ACHJZ2_002110"
     CDS             complement(3009..3404)
                     /gene="rpsF"
                     /locus_tag="ACHJZ2_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO4568217.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            3731..4006
                     /gene="yjfY"
                     /locus_tag="ACHJZ2_002111"
     CDS             3731..4006
                     /gene="yjfY"
                     /locus_tag="ACHJZ2_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO4568218.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(4135..4821)
                     /gene="ulaF"
                     /locus_tag="ACHJZ2_002112"
     CDS             complement(4135..4821)
                     /gene="ulaF"
                     /locus_tag="ACHJZ2_002112"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO4568219.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDISGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4821..5675)
                     /gene="ulaE"
                     /locus_tag="ACHJZ2_002113"
     CDS             complement(4821..5675)
                     /gene="ulaE"
                     /locus_tag="ACHJZ2_002113"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO4568220.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5685..6335)
                     /gene="ulaD"
                     /locus_tag="ACHJZ2_002114"
     CDS             complement(5685..6335)
                     /gene="ulaD"
                     /locus_tag="ACHJZ2_002114"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO4568221.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(6349..6813)
                     /gene="ulaC"
                     /locus_tag="ACHJZ2_002115"
     CDS             complement(6349..6813)
                     /gene="ulaC"
                     /locus_tag="ACHJZ2_002115"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO4568222.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6823..7128)
                     /gene="ulaB"
                     /locus_tag="ACHJZ2_002116"
     CDS             complement(6823..7128)
                     /gene="ulaB"
                     /locus_tag="ACHJZ2_002116"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO4568223.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(7144..8541)
                     /gene="ulaA"
                     /locus_tag="ACHJZ2_002117"
     CDS             complement(7144..8541)
                     /gene="ulaA"
                     /locus_tag="ACHJZ2_002117"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO4568224.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            8896..9960
                     /gene="ulaG"
                     /locus_tag="ACHJZ2_002118"
                     /pseudo
     CDS             8896..9960
                     /gene="ulaG"
                     /locus_tag="ACHJZ2_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
     gene            10068..10823
                     /gene="ulaR"
                     /locus_tag="ACHJZ2_002119"
     CDS             10068..10823
                     /gene="ulaR"
                     /locus_tag="ACHJZ2_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO4568225.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10820..11568)
                     /gene="yjfP"
                     /locus_tag="ACHJZ2_002120"
                     /pseudo
     CDS             complement(10820..11568)
                     /gene="yjfP"
                     /locus_tag="ACHJZ2_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
     gene            11750..12079
                     /gene="bsmA"
                     /locus_tag="ACHJZ2_002121"
     CDS             11750..12079
                     /gene="bsmA"
                     /locus_tag="ACHJZ2_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO4568226.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            12228..12503
                     /gene="yjfN"
                     /locus_tag="ACHJZ2_002122"
     CDS             12228..12503
                     /gene="yjfN"
                     /locus_tag="ACHJZ2_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO4568227.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12620..14245)
                     /gene="aidB"
                     /locus_tag="ACHJZ2_002123"
     CDS             complement(12620..14245)
                     /gene="aidB"
                     /locus_tag="ACHJZ2_002123"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO4568228.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(14329..15492)
                     /gene="yjfC"
                     /locus_tag="ACHJZ2_002124"
     CDS             complement(14329..15492)
                     /gene="yjfC"
                     /locus_tag="ACHJZ2_002124"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO4568229.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(15495..16133)
                     /gene="yjfM"
                     /locus_tag="ACHJZ2_002125"
     CDS             complement(15495..16133)
                     /gene="yjfM"
                     /locus_tag="ACHJZ2_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO4568230.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(16143..16541)
                     /gene="yjfL"
                     /locus_tag="ACHJZ2_002126"
     CDS             complement(16143..16541)
                     /gene="yjfL"
                     /locus_tag="ACHJZ2_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO4568231.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(16559..17218)
                     /gene="yjfK"
                     /locus_tag="ACHJZ2_002127"
     CDS             complement(16559..17218)
                     /gene="yjfK"
                     /locus_tag="ACHJZ2_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO4568232.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKENHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(17269..17967)
                     /gene="yjfJ"
                     /locus_tag="ACHJZ2_002128"
     CDS             complement(17269..17967)
                     /gene="yjfJ"
                     /locus_tag="ACHJZ2_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO4568233.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(17986..18387)
                     /gene="yjfI"
                     /locus_tag="ACHJZ2_002129"
     CDS             complement(17986..18387)
                     /gene="yjfI"
                     /locus_tag="ACHJZ2_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO4568234.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(18514..19245)
                     /gene="rlmB"
                     /locus_tag="ACHJZ2_002130"
     CDS             complement(18514..19245)
                     /gene="rlmB"
                     /locus_tag="ACHJZ2_002130"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO4568235.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(19426..21867)
                     /gene="rnr"
                     /locus_tag="ACHJZ2_002131"
     CDS             complement(19426..21867)
                     /gene="rnr"
                     /locus_tag="ACHJZ2_002131"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO4568236.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(21906..22331)
                     /gene="nsrR"
                     /locus_tag="ACHJZ2_002132"
     CDS             complement(21906..22331)
                     /gene="nsrR"
                     /locus_tag="ACHJZ2_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO4568237.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(22536..23834)
                     /gene="purA"
                     /locus_tag="ACHJZ2_002133"
     CDS             complement(22536..23834)
                     /gene="purA"
                     /locus_tag="ACHJZ2_002133"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO4568238.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(23938..24135)
                     /gene="yjeT"
                     /locus_tag="ACHJZ2_002134"
     CDS             complement(23938..24135)
                     /gene="yjeT"
                     /locus_tag="ACHJZ2_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO4568239.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(24217..25221)
                     /gene="hflC"
                     /locus_tag="ACHJZ2_002135"
     CDS             complement(24217..25221)
                     /gene="hflC"
                     /locus_tag="ACHJZ2_002135"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO4568240.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(25224..26483)
                     /gene="hflK"
                     /locus_tag="ACHJZ2_002136"
     CDS             complement(25224..26483)
                     /gene="hflK"
                     /locus_tag="ACHJZ2_002136"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO4568241.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(26569..27849)
                     /gene="hflX"
                     /locus_tag="ACHJZ2_002137"
     CDS             complement(26569..27849)
                     /gene="hflX"
                     /locus_tag="ACHJZ2_002137"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO4568242.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(27926..28234)
                     /gene="hfq"
                     /locus_tag="ACHJZ2_002138"
     CDS             complement(27926..28234)
                     /gene="hfq"
                     /locus_tag="ACHJZ2_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO4568243.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(28320..29270)
                     /gene="miaA"
                     /locus_tag="ACHJZ2_002139"
     CDS             complement(28320..29270)
                     /gene="miaA"
                     /locus_tag="ACHJZ2_002139"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO4568244.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLRGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(29263..31110)
                     /gene="mutL"
                     /locus_tag="ACHJZ2_002140"
     CDS             complement(29263..31110)
                     /gene="mutL"
                     /locus_tag="ACHJZ2_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO4568245.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(31120..32457)
                     /gene="amiB"
                     /locus_tag="ACHJZ2_002141"
     CDS             complement(31120..32457)
                     /gene="amiB"
                     /locus_tag="ACHJZ2_002141"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO4568246.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(32476..32937)
                     /gene="tsaE"
                     /locus_tag="ACHJZ2_002142"
     CDS             complement(32476..32937)
                     /gene="tsaE"
                     /locus_tag="ACHJZ2_002142"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO4568247.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(32909..34456)
                     /gene="nnr"
                     /locus_tag="ACHJZ2_002143"
     CDS             complement(32909..34456)
                     /gene="nnr"
                     /locus_tag="ACHJZ2_002143"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO4568248.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            34455..35594
                     /gene="queG"
                     /locus_tag="ACHJZ2_002144"
     CDS             34455..35594
                     /gene="queG"
                     /locus_tag="ACHJZ2_002144"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO4568249.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(35577..35630)
                     /locus_tag="ACHJZ2_002145"
     CDS             complement(35577..35630)
                     /locus_tag="ACHJZ2_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568250.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         8689 a         9816 c         8741 g         8620 t
ORIGIN      
        1 ggtcttaact ggaatttaaa tgcctgtagt cgtttcacag gccaaatgat actttggtct
       61 gtgaaaaaag aaaccgatat ccggcgtgga aggcattgtg ttttcctgat gcatgttcac
      121 ctggtctttg tcaccagata ccgacgccag atttttgacc atgacgcgac agaaaaacta
      181 cgcacttact tttcaaaggt atgtgctgat tttgaagctg aactggttga aatggatggc
      241 gaaccagatc acgtccattt gttaattaac tatcctccca aactggcgat atccagtctg
      301 gtaaacagcc tcaaaggcgt atcgggtagg ttactgcgac gagatcgacc agatattgca
      361 gtcaggtatt actacaaagg cgttttgtgg agtcctggct attttgccag tagctgcgga
      421 ggtgcgccaa tatccgccat ccgtcaatac attgaacaac agcaaacacc tggttaggtg
      481 gaaaaccgcg ccgtatatcc ccgccctgaa ggacggggtt ttacggcgca ccggataaac
      541 caggccaagc gtatgctggt tgatcaaata atatccccca agcgcagcaa tattaatatt
      601 cacctgcaat gggtcgatat ctggcttaaa aatattgtat ttcttccctt catccaataa
      661 ggattccatt aactgcaagt gcgcatggtt gatttctaag agacgctgtg attttgcata
      721 atgcacacct ttactttgat tttcactatg cacaattttt aaaaaccagg ggttagcaat
      781 ataataatcc catgtaaaat caataatttt ctcaatggct ttttgtggct ggttaacctg
      841 ctgattaacg aatacaccga taccgacagc gtagaataat tacataccgc acaacaagaa
      901 tgtacggtgt aaggcgtgga agtcattttc tccccgggtg catcgttata tccataatga
      961 gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca gaaaacaaac
     1021 ttatttttat taatatcgat ttttttctgt gaacattcag ctgcgatatt tataaaaaca
     1081 ttaaacaggt cacattacaa tgacattacc aaccactatt tattcatttc ctgcatattt
     1141 atctcgtttt tcgtccaccg acaaaccagt aaaattaaaa tttcatcaat acgcacgcgc
     1201 tacattactt tcaaatcgtg gaagagatca taattgcgat ggcagacgta ctgttgagat
     1261 ccacaaacta gatctctcag actggcaagc attcaataag ttagcaacga agtgtaatgc
     1321 ttacgatgga gtaacaatga atggtgataa ttcttttgga tggaaccatg aagctacatt
     1381 agataatatt cacgcccaga aatacaataa agcttacgcg ggtgcgaggt taaccgcaga
     1441 actgaaatat ttattgcaag atgtcgaatc cttcgaaccc aacagtaaat acacaataca
     1501 cgaagtggtt ttgggacctg gttatggtac tccagactat accggccaaa cgataggata
     1561 cgttgtaacc cttcctgccc aaatgcctaa ttgctggtca tctgaactac caacaatcga
     1621 tctttatatc gaccaacttc gcactgttac aggcgtttct aatgccctcg gattcatcat
     1681 tgctgcattg ctaaacgcgt attctgattt accccatgat ttgaaaattg gtctgcgttc
     1741 cttatcaagt tcagccgcaa tctactcggg tttgggtttc gagcgagtgc cgcaggagcg
     1801 agacatcagt tgcgctcgta tgtatctaac gccagcaaac catcctgact tatggacaca
     1861 agagaatggt gaatggatct acttgcgaaa ctaatacctg acgaaaaata gaaagcaaaa
     1921 cgccgaccag tggtcggcgt ttttacgtct cgttgaataa cgaattattc agctactacg
     1981 tttacgatca ctttcgcgaa tacttcgctg tgaacctgga agctcacttc gtgttcgcca
     2041 gtggtacgca gaacgccgtt cggcagacga acttcgctct tagccacttc aacgccagct
     2101 gcagttacag cgtcagcgat gtcgcgagta ccgatggaac cgaacagttt accttcgtcg
     2161 ccagctttag acgcgatggt aacagtttcc agtgcattga ttttctcagc gcgagcatta
     2221 gcagctgcca gaacttcagc cagtttagct tccagttcag cgcgacgtgc ttcgaagaat
     2281 tcaatgtttt tcttggtagc tggaacagct ttaccctgtg gtaccaggaa gttacgagca
     2341 tagcccgctt taacgtttac ctgatcaccc aggctaccca ggtttgctac tttatcaagc
     2401 agaataactt gcattacctt atcctctcaa agtcgtatta atggaccgtg accgattact
     2461 gatggcgatc agtgtacggc agcagggaca ggtagcgagc gcgtttgata gcgcgagcca
     2521 gctgacgctg gtattttgca cgggtaccgg tgatacggct tgggacaatc ttaccgcttt
     2581 cggtgatgta gtttttcagc gtagcgatat ctttatagtc gatctcttga acgccttccg
     2641 cggtgaaacg gcagaacttg cgacgacgga aataacgtgc catatggcta gtctccagaa
     2701 tctatcaatt caatctgctc ggcatgcaaa accattttgc tcagtccgtt ctttgccttg
     2761 tggcatgaaa tgaacccctg aacggttatg cgactgccga ccgttatact gtgagtaatg
     2821 gcctggtttt cgtgtccgct aacaataacg ggcatttgac accatgcctg ccggtgaaag
     2881 ccggcttcct cctgcacaga acgatgctca agcacgaact ggcagtgagg aattcctgat
     2941 ggactgacct ttcgaagggg agtcctgcac acggtgccgg acaacaccag acggttggtc
     3001 atcagaaatt actcttcaga atccccagct tcagcatcat cagcggtttc gtttgcgaaa
     3061 tcatcgcgac gctcacggcg ctcgtctttc gctttaacca tcggagatgc ttcggtaaca
     3121 gcgtgcttgg tacgcataac catgctgcgg ataacggcat cgttgaagcg gaaggtagtt
     3181 tccagctcat cgatcacttc ctgcggagct tcaacgttca tcagaacgta gtgagctttg
     3241 tgcagtttgt tgatcgggta agccagctga cggcggcccc agtcttccag acggtggatc
     3301 ttgccttctg caccagtgat ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg
     3361 ctctggtcag gatggaccat aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg
     3421 gattattcag cctcctgtct gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag
     3481 gtcggctgaa aaatgacgcg ttatagtact gacatacccc gctaaactca aggccagccg
     3541 acaaataaat ctcatctttc accaaagtgg actaacacgc tgatttgaag ttcatcacac
     3601 ttcatcatgg tgttttttcg aacaactgca tcagaaatgg tcaccatcaa cttctttatc
     3661 attgattaaa attaaatcag aagatcatca cagaggagag cacacaaagt cagccgtcga
     3721 ggaggtatct atgttcagtc gagttttagc ccttctggct gtgcttttgc taagtgcaaa
     3781 tacatgggca gccattgaaa ttaataacca ccaggccaga aatatggacg atgtgcaaag
     3841 cttaggcgtg atttatatca atcataattt cgccactgaa agcgaagcac gtcaggcatt
     3901 aaatgaagaa acagatgcgc aaggcgcaac gtactaccac gtaattctga tgcgggaacc
     3961 ggggagtaac ggcaatatgc acgccagcgc ggatatttat cgctagcacc aggtataacc
     4021 aacgaaacat tgccatagtt tgctttgccc ccttcgcagg gggctttttt tgaaaaacgg
     4081 ctgcggaatt agaccagtta tctcccgagg aaggaaattt ccgcagcgcg tgttctactt
     4141 ctgcccgtaa taagcgttgg ggccgtgctt acgcatgaag tgtttattca tcaggaagct
     4201 gtcgatgtga ttgagttgtg gattaatact gcgcgcaatc cacgccattt tcgccacttc
     4261 ttccatcact accgcgttat gcaccgcgtc gtgggcatct ttcccccagg cgaacggtcc
     4321 gtgctgatac accacaattc ccggcgtatg cagcggctcg gcgttgccca gcgtttcgat
     4381 aatcactttg ccagtgttca gttcatactc accctgcacc tcttcttcgc ttaatccacg
     4441 cgtacacggg atatcaccaa agaagtagtc agcgtgcgtg gtgcctaacg ccgggatcgc
     4501 cagccccgcc tgcgcccatg cggtagcatg agtggagtgg gtatggacaa tgccgccaag
     4561 cgacggataa cgacggtaga gttcgagatg cgtcgcggtg tcggaagacg gacgatattc
     4621 cccttccacc accttaccgc ttatatcgac caccaccata tcgtctgctt tcatggtttc
     4681 gtaggcgacg ccgctgggct tgatcaccac cagcccgcgt tcgcggtcga tagcgctgac
     4741 gttgccccag gtaaaggtca ccagcccata gcgcggcaga tccatgttgg cttcaaacac
     4801 ctgctgtttt agcttttgca ttatgccgcc tccaccatgc ccgctttcgc catgcgcgct
     4861 ttcacccaat cacgcgcttt cgccacttct gccgccgggt cttccgccgt ttcgctccac
     4921 atctcaatca ggtacggccc gcaatagcca ctctgtttga gcgtttcgaa gcaacgttcg
     4981 aaatccacca caccttcgcc aaatggcacg tttttgaaga cgcccggttt ggtgtctttc
     5041 acatgtaccg cgacgatatg cccgattccg gcctgcaact ccatctgcac atcgttgtcc
     5101 cacgccgaca ggttgccgat atccgggtag agctggaacc acggattgtt gagatagtgc
     5161 gcgtagccca gcgccttgct gatggagttc atcaacggat aatccatgat ctccatcgcc
     5221 agcgtcacct gcgcgcggct cgccatctca acgctctctt tcaggccgtc acggaaacga
     5281 cgacgcgttt cgttattggc ttcctgatag taaacgtcat agcccgccag ttggatcacg
     5341 cgaataccaa cgtcctgggc gaactggata gctttacgca taatctccag cccctgcgcc
     5401 cgcaccgcgt catcttcact gcccagcggg aaacgacgat gagcagaaag gcacatggac
     5461 ggcacgcgca cgccggtttc aacaatcgca ttcaccagcg ccagacgctg ctcgcggctc
     5521 cagtcgaggc gcgacaggcg ctcgtccgtt tcatctaccg acatctcgac aaaatcgaag
     5581 cccaacgttt ttgccagttg caggcgttcc agccagcact ccccggcggg gagcgctttt
     5641 tcatagatgc caagcgggat ttgtttggac aacatatccg ctccttagcc ccacagttcg
     5701 gcgatggaac gtttgaactg acgtgcggct tccaccggag aagcggcatc acggatacta
     5761 cggcccgcga taaagacgtg aatcggaatg cctttgaaca gcggcagatc ttccagcgcc
     5821 aggccaccag tgacggtgac tttgaagccc atatcggaaa gacgtttgat cgcggtgatg
     5881 tccgcttcgc cccacgccac gcctgcggcc tgcgcgtcac ggctgcggtg ataaaccacc
     5941 tgctgaatac ctgcgtcgcg ccactgttgc gcctgttccc aggtccagta accggtcagt
     6001 tcgatctgca cgtcgccgtt aaactctttt gccacgtcca gcgcgccttt ggcggtgttg
     6061 atatccgcac agcaaattac cgtcacccag tcagcgttgg cttcgaagca catacgcgaa
     6121 aggattttgc ctgcatcggc aattttggcg tctgccagta cgattttgtg cgggtagagc
     6181 gctttcaggt cacgaaccgc acgcacgcct tcgcccacgc acagaatggt gcccacttcg
     6241 ataatgtcga cttcttcggc aatcaggcga gtggtttcgt aggcgctatc catagtctgg
     6301 ttgtccagcg cgacttgcaa catcggtaat gacatgttca attccttctt aagctgccgc
     6361 gttggtgcgg tcaatgagat ccagtacttc ctgctcggta cggcaggcgc gtaaacggtc
     6421 aaaattctct tcatcttcaa acaggttgac gatctgcatg atgcccactt cctggtgagt
     6481 attggcatcg accgccgcca tggtgatgag gatatccacc ggatcgttgt catcgtggtt
     6541 gaactccagc ggctttttca gcgttaccag agagaaaccg gttttcttaa cgccctcttc
     6601 cggacgcccg tgcggcattg ccaggcccgg agcgataacg aaataaggac cgaactgttc
     6661 aacgccatcc agaatcgcct ggtagtaacg cggctctacc acatctgccg ccaccagcag
     6721 gtcaacgccg attttcaccg cttcctgcca tgtctctgct tcagcctgca gacggatgga
     6781 tttattttca gccagcgaat cacgtaattt catggcgcgt ccttacttca cgtcctgcgg
     6841 gaaatgctct ttgatcactt ccagcagttt cgggccaaag tcggcaggag agagcatgtt
     6901 gcgcacgcca accacgtatt tgttgccggt cacggtgatt tcgcccgcaa tgtgcgtaga
     6961 agcgatgatg atatccgcgc cactcaactc gcttttgtac tcgccaaccg cgcagctgtt
     7021 taccgtatgg tcaatgtttg attgggttaa aaactggtcc actttcatct tcatgatcat
     7081 ggaactgcct tgtccgttgc cacacacagc cagaatacgt acggtcataa tcaaaactcc
     7141 ttattaagca gactgttctg ccagttgttt ttctgcatct tcttctgcgc gcagcgcgcg
     7201 gccagcgaag aacatataag ccagtgcaat tacaatgatg acggccataa aggcgatacc
     7261 gatggagaag aagccttgca tcatcggcgg tgccagaatc gaccagtccg ccatgcccat
     7321 ccaggcactc ataccggtga gtttcaccgc ccagacgcaa ccaaagattt cgatcattcc
     7381 catcaccaga caaatcttca gcgctgcacg ccagccgccg aagtggttag cgaacacgcc
     7441 gatggtggcg ttggagaaga acatcgggat aaagcctaga ataatcagaa tggaagagcc
     7501 gcaggcgacc aggatgccaa ctgcaatcag ctgaccgatg gtgccccaca taaagcccca
     7561 gaccacggcg ttcggcgcga agctatagat agccgcacag tcaatcgcca gaaccgcacc
     7621 tgggatcagg cgctgggaga taccgttaaa tgcttcagag agttccgcca caaacatgcg
     7681 cacgccctga gtgatgatga agatcgccac cgcgaaggag aaaccggttt gcaggatgta
     7741 gaccgtccag tgcactttgc ccgccatcgc ctgcacggtg tcgataccga aggagagcag
     7801 aatggcgcca aagaagatgg tcatcacaat cgccgtggag acgatgttgt cgtggaaaat
     7861 gttcagccag cccggcagtt tgaggtcttc aacgctctcc tcttttttgc cgaggaacgg
     7921 cgcgacttta taggcaatcc atgatgcaaa ctgctgctgg tgaccgatgg agaaaccaca
     7981 gccatccgtc acttcctgag tcggcttgta catcatgttg gaagtgatgc cccagtagag
     8041 cgaaaccaga atcgcggtac agataatggt ggtccacatg gagtagccga agataaacag
     8101 cgtaacggca atcaacccgg cctgctggaa catgatgtgg ccggtcaaca tgattgtgcg
     8161 aatgccggta atgcgacgca acagcacgta acagatgttc agtgccagcg ctaacaatac
     8221 ggcgtaaccc acccagctat aggcatcgcc catgcggtcg atggttgcca tcattgaagc
     8281 gtaggtatcg gaaattgcgc cgttaatgcc gtagacttcg gacattttcg ccaccaccgg
     8341 tttgaaggtg ctggtgagga tgccggaccc tgcctgcaac aacatgaaac caattatggt
     8401 tttaatcgtg cctttaataa taacgctgac acttttgcgc agtaggatgt agcccagaca
     8461 ggtcacaata cccagcaaca acggggcatt ggtcatgacc tggttaaaaa atacggtaaa
     8521 gatgttgtag aggatctcca taacgatctc ctgaagaaga ggtaaccggg tattccacac
     8581 acccggaatg ttgtgcactc actctaattt tcaaaagtaa tcacaacaag attatttatg
     8641 attaaatgtg acgcgacccg caaataattc cacatggata gagcgggatt cacgcaacga
     8701 aacaattatt catttaaatt catttggtta attgaattat acattccatt aaatctttcc
     8761 atactcaaaa tcaacaacaa cttccctggc aaaaagtcaa aaaatggtca ttgccttcca
     8821 gaaaaaatag tgtcaggatt aatcaaaacc aatcacatat tgattcgaat tgaactatca
     8881 tgaaggtaaa tggcgatgag taaagtgaaa agtatcaccc gtgaatcctg gatcctgagc
     8941 actttcccgg agtggggtag ctggttgaat gaagaaattg aacaagaaca ggtcgctcct
     9001 ggcacatttg cgatgtggtg gcttggctgc accgggatct ggttgaaatc ggaaggtggc
     9061 accaacgttt gcgttgattt ctggtgcggc actggcaaac aaagtcacgg taacccgtta
     9121 atgaaacagg gtcaccagat gcagcgcatg gctggcgtga aaaaactgca gccaaacctg
     9181 cgtaccaccc cgtttgttct tgatccgttt gcgattcgcc agatcgacgc ggtactggcg
     9241 actcacgatc acaacgatca tatcgacgtt aacgtcgctg ctgccgtgat gcagaactgt
     9301 gctgatgacg taccgtttat cggaccgaaa acctgtgtgg atttgtggat tggctggggc
     9361 gtaccgaaag agcgttgcat cgtggtcaaa ccgggcgatg tagtaaaagt gaaagacatt
     9421 gaaattcatg cgcttgatgc tttcgaccgt actgcactga tcaccctgcc tgccgatcaa
     9481 aaagcggctg gcgtactgcc agatggcatg gacgatcgcg cggtgaacta cctgttcaaa
     9541 acgcctggcg gctccctgta tcacagcggc gactcccact actctaacta ttatgcgaag
     9601 cacggtaacg aacatcagat cgacgtggcg ttaggatcgt acggcgaaaa cccgcgcggt
     9661 atcaccgaca agatgaccag cgccgatatg ctgcgtatgg gtgaagcgct gaatgcgaaa
     9721 gtggttatcc cgttccacca cgatatctgg tcaaacttcc aggccgatcc gcaagagatc
     9781 cgcgtgctgt gggagatgaa aaaagatcgt ctgaagtatg gcttcaagcc gtttatctgg
     9841 caggtgggcg gcaaatttac ctagccgctg gataaagaca acttcgagta ccactatccg
     9901 cgcggtttcg atgattgctt cactattgaa ccggatctgc cgttcaagtc attcctgtaa
     9961 tctgtcgttt ataccggata gcgtggagaa gttcgcgcta tccggcgtag tatttcctgg
    10021 ctatttcaaa tatcatctaa aaaaatcaaa ttttatcgga atagctcatg actgaagcac
    10081 aaagacatca aatcctcctg gaaatgctcg cacaattggg ctttgtgacc gttgagaaag
    10141 tcgttgagcg tctgggaatt tcgcctgcca ctgcgcgacg cgatatcaat aaacttgacg
    10201 aaagcggcaa actgaaaaaa gtgcgcaatg gtgcagaagc tattacccaa cagcgcccgc
    10261 gctggacgcc gatgaatctg catcaggcgc agaatcacga tgaaaaagta cgtatcgcta
    10321 aagcggcctc gcagctggtt aatccgggcg aaagcgtagt catcaactgc ggctccaccg
    10381 cgtttctgct tgggcgggaa atgtgtggca agccagtgca aatcatcact aattatctac
    10441 cgctggcaaa ttacctgatc gatcaagaac atgacagcgt gatcattatg ggcggacagt
    10501 acaacaaaag tcagtccatc actttaagcc cgcagggcag cgaaaacagt ctctatgccg
    10561 ggcactggat gtttaccagc ggaaaagggc tgaccgcaga agggttgtat aaaaccgata
    10621 tgctgacagc aatggcagag cagaagatgc tgagcgtggt agggaaactg gtggtactgg
    10681 ttgatagcag taagattggc gaacgcgcgg gaatgctttt tagccgtgcc gatcaaatcg
    10741 atatgcttat caccggcaaa aatgctaacc cggaaatcct gcaacaactg gaagcgcaag
    10801 gggtcagcat tctgcgtgtt taaagatgct ggcggaaaaa tgtcacggca gcatcaacgc
    10861 ctcaggcgta atgcggtgac gcacgcctgg ctgccatgaa caggttagca gtttatcccg
    10921 tcccgtctcg cttaaggcct gctgcaaacg tagtgattcg tcggcaggca caacgtcatc
    10981 gtcgaggcca taccacagca gtagaggtct gtcactaagt tgttccaggt ggtttgtcgc
    11041 ttcccactct gccagtggcg cgacaatgtt attgaattca ttctgctgtg ctaccgtttc
    11101 aggtatcagc ggtggaaaca gtgaacgggc gagtgatgta aaatagcccg atcccatcat
    11161 gctggcggta catctcaccg tggggtggcg agcggtaatc cccagtgccg tcatcgcgcc
    11221 catcgacgca ccaccgactg ccagacggtc atcaagcagc cacttttctt cggctattgc
    11281 cgcacgtaaa gtagtgaatt cctgcatact ttgtagcaag atttgccaga attgatttaa
    11341 ccgccgcgct gcgtcaccac taaaacggct accgtgatcg ggcgcatccg gcatgatcac
    11401 ccgcaaacca gcctgcgcca gcgcaacggc aaaatagcta tacaccagac tggacgaagt
    11461 aaagccgtga taaaaaatca cgcacggtaa cggggtatct ttttgtccga caggataagc
    11521 atgaagaacg ggaatatctg ccagctcgcg tgattctatt tcaatcatct gtctctcctt
    11581 tttgtctggt atgacatgaa gaaatcctta gcacaagttt ctgctggcgg tattagttca
    11641 taatgttgag atgtgggtta cgctttcgtc acgttttcat tcgaaaatcg cgtaccaggt
    11701 aacaattcgg gaacattccc ccaaagttaa attaataagg cactacacta tggttagcag
    11761 gaaacgtaat agcgttattt accggtttgc cagtttatta ttggtgttga tgttaagtgc
    11821 ctgtagcgca ctgcaaggta cgccacagcc agcaccgcca gtaacggacc atccgcaaga
    11881 gattcgccgc gaccagacgc aagggttaca gcgaataggt agcgtaagca caatggttcg
    11941 gggttctccg gatgacgcat tagcagaaat cagagcaaaa gctgtcgctg caaaagctga
    12001 ttattacgtt gtcgtcatgg tggacgaaac catcgtgaca ggacagtggt attcacaagc
    12061 cattttgtac cgtaaataaa tcagacaact tacatagtca gctttacatt gctttgcgtc
    12121 cctatgcgtt tacctgtgca caataaatta ccggctggaa gcctgggcgt ttattaccgc
    12181 gatggaatgc cctgcaacat gtggggaaac gaaaaatgga gctgacgatg aaacaattac
    12241 ttgcctcacc ctcgctgcaa ttagtgactt atcctgcgag cgccacggca cagtctgcag
    12301 aattcgccag tgctgactgc gtgacgggcc tgaatgaaat aggtcagatc tcagttagca
    12361 atatttcagg ggatccgcag gacgtggaac gtatcgtggc gttaaaggcc gatgaacaag
    12421 gtgcgtcatg gtaccgcatt attaccatgt acgaagacca gcagcctgat aactggcgcg
    12481 tacaggcgat actgtatgca tgagtgtcat ctacagcaaa tgaacaatgc cggatgcggc
    12541 gcgagcgcct tgtccggcct acaattttca catatttcat ttagttaatc gaaaccagcg
    12601 tcgcatcagt cgtatacgct tacacacaca ctccccccgt cgcccgcagc aataaatcat
    12661 tctggatctg ctctgacaac cgtacgccgc cgcgcgtatc taacatcacc tgacaccacg
    12721 cctgcgccat tggcggagaa gcatatttca acatttgcgc accgcagccc agcaggaata
    12781 gctgatgagt aatctctcgc cccagttctt cagctggctt acgcagctgc tgctgtaaac
    12841 gacgaaccgc gcaatcaaaa tagcgatcct gccctttcac ttctacaaat gcttccgaca
    12901 ataagtcgta tacgcccgct tgcttattga gaacgcgcag cacatccagg cacataatat
    12961 tgccggaacc ttcccaaata ctgtttaccg gcatctcccg gtaaagccgc ggcagctcgc
    13021 tctcctcgca ataaccaatg ccgcccagca cctccatcgc ttcggccaca aacggcatac
    13081 ctcgtttgca gatcacaaat tttgccgcag gcgtaaataa acgcgcccac agagcttctt
    13141 tggcatcggc gcgccggtcc cacgctcgcg caagacgaaa cagcaacgcc gtttgccctt
    13201 caagctgaag tgccatgcga cttaagacat gacgcataag gggctgttgg atcaatggat
    13261 taccaaaaac atggcgttga tgcgcatgat aaatcgccag cgagaacgca cggcgcatca
    13321 tggcatggct acccagggcg caatcaaaac gcgtcatccc ccccattttc aggatcagac
    13381 gaattccttc cccttcctgc cccaataacc agccaatggc atcctgaaac tccacttcac
    13441 aactggcgtt agagcgatta cccagcttat ctttcagccg ctcgaggcga atcgcgttgc
    13501 gttgcccgtc aggcaaaaag cgcggcacaa aaaagcagga caaaccgccc gcggtctgcg
    13561 ccagcaccag atgcgcatcg ctttgcggaa ctgagaaaaa ccatttatgc cccaccagcc
    13621 gataagagcc atcctccaga cgctctgcgc gggtggtgtt gctcatgaca tcggaaccgc
    13681 cctgcttttc cgtcattccc atgccaatca acaaaccgcg tttttgccca cctggcaata
    13741 agtgagaatc gtagcgatcg ctcaacagcg gtgtggtcca gtcctgaaac ggcgcgggta
    13801 acatctgtaa caacaatggc gtggcggcaa aggtcatggt tatcggacat aacgaccccg
    13861 cctcaacctg tgcgtgcaac ataaaacgcg ccgcgcgcgc cacaaatgcg ccggagcgag
    13921 cgtcttcttc ccaggcaaga ttgtgcaccc gattggtaca tagtgcctgc atcagcaggt
    13981 gccaggcggg gtgaaaacgc acatcgtcca gacggcgtcc ttgcgcatcg tagcgcaaca
    14041 gttcaggcgg attcacattc gccagccgcc ccagttcaag ggattcagcc gttcctaact
    14101 gctgaccaat actggctaga aaatcgctat cccagccagc accttcacgc gttaccgctt
    14161 cgcaaagcgc gccatcagac aggtataagt tgctgttatt taatggtata ggttgattaa
    14221 aaacggtgtg agtttgccag tgcactgtgt ctccctccat caatggcagt caccattagt
    14281 atggtcactg ccatggattc atgacagaaa gagattgcta aaacattctt atccagcaat
    14341 gtaatgcgga acgaaacgtg aggtgtcttt ggtgatcagt gtgttatctt cacggatccc
    14401 cattccgcac gcttcatcat cgacaatcca gctaccgatg agtgtgtagc tatcgccaaa
    14461 ccgcggcaga ggttgaaacg cctggtagat catcggttca tcggcgtaat caccatcagc
    14521 gtggtcaacg acgttattct ggccgtcgaa aatggtgaca tttccgcctt cgcgcgagta
    14581 gatcggttta cgcacatagc tttcgccagc ggcaatctgc ggtttttcac cctcaaacca
    14641 ggatgcaaga aggttaggat gaccagggaa gaagcgccaa agcagcggca ttagcccttt
    14701 attactcaag atacttttcc ataacggctc cacccattgc tcgcgacgct tgcgcagcag
    14761 cggaccgtta tcgtcacgca tcatccactc cagcggatac agcttaaatg cacgctggat
    14821 gacattatca tcaagatcgg tcagtacgcc gccgacgccc aaaccgagat cttcaatgta
    14881 gataaaccgc gactcctgcc ctgcctgctg ggcgcagtcc tgcaagtaca gcacggtact
    14941 cctgtcttca tcggtgtcct gacagcagca aaaataaaac ggttcccgac tgtaaagctc
    15001 gctaaagcgt gaaatcaggc gttcctgaat agcattgtac tgatcggcat cacgcggaat
    15061 aatgccgctg cgccgggcat cctccagcca cagccactgg aaataagccg actcgtacaa
    15121 tgaagttggc gtatcggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc
    15181 aaaatccatc cgtccataca gcgaaggatc gcgggcgcgc cagctttcag cgatcacatc
    15241 ccagtacaac ggcgggatcg ccagttgcgt caggatctct tcatctttta ccgcgcgatc
    15301 caccacctca aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat
    15361 ctggcgcaga gtaaaacggt aagcccgact ctcatcccaa tagatttcat tgtcgatgat
    15421 atgaaagtcg aaaccgttgt cagcggcgat ctggtccaga tcccgtcgca caggaacgtt
    15481 gtgtctcagc atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg
    15541 ccgcgagaaa cggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt
    15601 ttgccagaac cggagcgcca ggagtaatca ccggaagtgt tgcgccagac ggggcgggaa
    15661 gcaaatgagg atccgccgct gttatagaca aacggctcat cgcgatcttt acgaataacc
    15721 cggcttaaca aaaatccaga aacgaccggg atccaactct gttcaacatt gtcgtagtag
    15781 caattttcgt acttagactg acagttctgc tgagtcatat tcttcggaac atcggcataa
    15841 aatgccgctt tggcgttgtt ccagccacgc gcgcaaatgt cggcattatt accgtcatca
    15901 atacaatcct gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac atcgccacta
    15961 tcgccacaac ctttcaatac aaaaaaagcg gcaccgccca ttagcgccaa cgttaagtat
    16021 ttttgtgcgt agcgattgcg acgcggttca aaaggattat tcggtctacc aatgcgactg
    16081 atcgctccgt gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc
    16141 cattaccatg tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata
    16201 aacattcctg ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt
    16261 ctgacaccag caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag
    16321 tctggaatac tcaccgcatt gattgccgca ctggataagg ggatcacgta acccaacaat
    16381 gtaccgctga acgccagtga cgctgcggta ttattgtttt tgattaattg ccattcattg
    16441 tgcggtgtaa ttttggagta gataaacaga aaaataatca ccatagccac gccaataaaa
    16501 aaataggcac taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt
    16561 aaccaataat atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc
    16621 actctggctc gccgagatcg ttaaaagatt cctcaccatt tagcagcaaa tattcgtaag
    16681 tatcttcggt cacttgccgc tggtagccca tggtaaaatt atggacttcc catttagcat
    16741 gattttgatt ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc
    16801 tgttaaaaaa tcgctgccag cgtttttcct gccagtttaa ggtcattgcc cccatcgttt
    16861 tggcgttgat cgcctcgcgc cagtgattct ctttactgat accgtagctc tcttcataga
    16921 caaagagctt gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca
    16981 gaaactcatc gcctgaagtg tagtagcgga aaatctgact accgccgccc agatcgatgt
    17041 ggctgacagc gactaccgta aattcctcac ccggcagcgc aatcagcaat tcatcttcca
    17101 gcaaacgaaa cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac
    17161 cgcgagcgat agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat
    17221 tttatcctta aaatagttgc cagccttgca gtgaggctgg caaaaaaatt actcgccctg
    17281 ttggcgttgc agtcttgcta atacatcctg ggcgctactt ttattgctac cgccaattcc
    17341 ggcttccgcc agcttttcgt caaggtcgcg accgtctgcg actttttcta actgtgcggc
    17401 agcatccagg cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc
    17461 tgctgtcgaa acgctggaag atgcgccaac ggtagaagtc gttacagcct gttgtgcacg
    17521 ctgcatggct tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg
    17581 cgctgtcgct gcaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac
    17641 ttgctcttca gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat
    17701 caacgacgga ttaacgttct tgcttaacgc ttccagcgta cgggcttcca gactggcttt
    17761 gcgctcacgt aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac
    17821 gcgagatttt ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat
    17881 gcgcacaccc tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag
    17941 cgtaaataaa ctttttaaaa ttcccataaa tacccgtcct tgtaattagt gtgaatattc
    18001 ttctgtaatt tcagccagat ccaatgcgtt atctaccagc gaggttatct caagcaggat
    18061 atcttcaaga gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcttgttg
    18121 tacgctggag attcctaccg atgacaacgg catcatcttc tgatttctta ataagaaggt
    18181 attaaattca tctggattgc tgatactact caccggacaa ataaaggttt cgataatcat
    18241 ttggcgggaa gtaaaaagaa tattgatttg caaatcgcca taatcattca ttttaataat
    18301 taatgcgttc tcgctatttg ttacatcaat attttgttca ggtacagttt gcagcgccgt
    18361 cgccagcgcc aacggattcc atgtcatata ttattccata tagattaagt ttaaatatta
    18421 ataaaatgaa tatttgcaat acgtaattat cttaccagct atagacaaaa aaaaaccatc
    18481 caaatctgga tggcttttca taattcagag gaactagctg cgctgacgaa ccgcttcaaa
    18541 taagcaaatt ccggttgcaa ccgaaacgtt cagggaagaa acacttcctg ccattgggat
    18601 gctgatcaac tcatcgcaat gttcacgggt caggcgacgc ataccttcac cttccgcgcc
    18661 catcaccagc gccaggcgtc cggtcatttt gctttgatag agcgtatgat ccgcctcacc
    18721 tgccgtaccg acgatccaga tattctcttc ctgcaacata cgcatggtgc gcgcaaggtt
    18781 agtcacccga atcagtggaa cgctttctgc cgcgccgcag gctacttttt tcgccgtggc
    18841 gttgagctgt gcggagcgat ctttcggcac aatcaccgcg tgaacgccag cagcgtccgc
    18901 gctacgcagg cacgcgccga ggttgtgcgg atcggttaca ccgtcgagga tcagcaggaa
    18961 cggttgatcg agcgaagcga tcagatccgg cagatcgttt tcctggtact gacgtcctgg
    19021 cttcacgcgg gcgataatgc cctgatgcac ggcaccgtcg cttttctcgt cgagatattg
    19081 gcggtttgcc aactggataa ccacgccctg ggactcaagg gcgtggatca gcggtaacag
    19141 acgtttatct tcacggcctt ttaaaataaa gacttcctga aaacgttcag gggcgcgctc
    19201 cagcagggcc tgcactgcat ggatgccgta aatcatttcg ctcattaatg tactcgttgt
    19261 ttacgttttg cctgatgcac tacgtttatc cggtctacat gatccctgca atatattgaa
    19321 tttgcgtgat tatgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaaagcgc
    19381 attttgtcag caatctaacc ctcttctgtt tgaagagggt aatgatcact ctgccacttt
    19441 tttcttcgcc gcacgcttcg ctttggtcgc tgcagctatt ttctgcgttt tcgccgatgg
    19501 ctttttcgct tttctcgcgt ctttcttcgc tgctttcggc ttcgtttttt tctcaccgcg
    19561 gaaggcgctg tctggctcaa agtttacctt tttaccgacc tgacgacgct tgccgccttt
    19621 tttacctgca tcgccttttt tcgctttctc gcgcgccgtt ttaccgacgt tgcgcggtgc
    19681 gcgttcgctg gagatcaggc taaagtcgat tttgcgctcg tccatattaa ccgcttcgac
    19741 gcgaacttcc acgcgatcgc ccaggcgata agtctggccg ctggattcac ccatcaggcg
    19801 ttgccctacc tggtcaaagc gatagtagtc attgtccagc gaagagacat ggaccagccc
    19861 atcaatgaac aagtcgtcca gacggacgaa gaagccaaag ccagtgacgc tggaaattac
    19921 gcctttaaag acgttaccta cctggtcgag catgaagtca cacttcagcc agtcagacac
    19981 atcgcgcgtt gcttcgtcgg cacgacgttc cgccatcgaa cagtgctgac ccaattgcag
    20041 catctcttcc atcgaataat ggtagccacc ggtttcagtg gtgttgccct gatgcccctg
    20101 ctctttcgcc agcagatact taatggcgcg gtgcagcatc aggtcaggat aacgacgaat
    20161 cggcgaagta aagtgcgcat aggactgcaa tgccaggcca aagtgaccac ggttttccgg
    20221 atcgtaaatc gcctgtttca tcgagcgcag cagcatggtt tgcagcattt ctgcatcagg
    20281 acggtcggca accgactcca gcagctccgc gtagtcgcgc ggttccggct tgttaccgcc
    20341 cggcagttcc agccccagct ctgccagcac tgaacggaaa gaggtaatcg cttcggtgct
    20401 cggcttgtcg tgaatacgga acagtgccgg ttctttcgct ttctcaacga aacgcgccgc
    20461 cgagatattc gccagaatca tgcactcttc aattaacttg tgtgcgtcgt tacgctgagt
    20521 ctgttcgata cgttcaatac ggcgttcagc gttgaaaatg aacttcgctt cttcgctctc
    20581 aaatgagatc ccgccgcgtt cttcacgggc tttatccagc actttataga ggttatgcaa
    20641 ctcttcgaga tgcttaacca gcggggcgta ctgctcgcgc agatcctgat cgccctgcag
    20701 aatatgccag actttggtgt aggtcagacg cgcgtgagag ctcatcaccg cttcgtagaa
    20761 tttgtagcca gtcaggcggc ctttcgacga aaccgtcatc tcgcacacca tacacaggcg
    20821 gtctacctgc ggattgagcg agcacagacc gttagagagc acttccggca gcatcgggat
    20881 aacctgcgaa gggaagtaca ccgacgtacc acggttacgc gcttctctgt ccagcggcgt
    20941 tggcggacgc acatagtagc tgacgtcagc aatcgcgacc cataaacgcc agccgccgcc
    21001 gcgttttttc tcgcagtaaa ccgcatcgtc aaagtcacgg gcgtcttcgc catcaatggt
    21061 gaccagcggt aaatcgcgca aatcgacacg gcccgctttt gcttcttccg gcacttcttc
    21121 tttcagccca gcaacctgtt gctcaacagc ctgcggccag atgtacggaa tttcatgggt
    21181 acgcagagcg atatcaaccg ccatgcccgt gcccatattg tcgcccagca cttcgacgat
    21241 tttacccacc gctttggtac ggcgagtcgg gcgttgggtc agttcaacaa ccaccacaaa
    21301 gcccatccgc gcgcccatga tctgatcggg cgggattaag atatcgaagc tcagacggct
    21361 atcgtcagga accacaaagc cgacgcccgc ttcggtaaag tagcgaccaa caatctggct
    21421 ggtttttggc accagtacgc ggacaatacg cgcttcacga cgacctttac ggtcagcacc
    21481 cagcggctga gccagcacct gatcgccatg aatgcaggtt ttcatctgct cgctggagag
    21541 atacaaatca tctttacgcc cttcaacccg cagaaagccg tagccatcac ggtggccaat
    21601 aacggtacct ttcaccaggt cgaggcgttc cggcagcgca tagcactgac gacgagtaaa
    21661 gaccagttga ccatcgcgct ccatcgcgcg caggcggcga cgcaggcctt caagctgctc
    21721 ttcgccttca atgtgcagtt ctaccgccag ctcatcacgg ctggccggtt tttcacgttt
    21781 ggttaaatgt tcgaggataa attcccgact agggatggga ttcgcgtatt tttcagcttc
    21841 gcgttcctgg aaaggatctt gtgacatctc ggttcctccg ttgtcatctc tgatgaagat
    21901 tttcgtcact ccaccagcaa taatttataa agcggttgat tctcttcaac caaatcggca
    21961 agcgtgtagt tatccagttc cgtaagaaaa ctttgcacgg ccttagaaag cgcctgtttc
    22021 aaccgacagg caggtgtaat gtggcaaaac tcactgctgc aattcaccag cgataagggc
    22081 tccagctcgc gcaccacatc accaatacgt atcgcactcg ccggtttacc caggcgaatg
    22141 ccgccatttt ttccacgaac agcagtcacg tagccggcac gactaagttg attgattatt
    22201 ttgaccatat gattacggga gacgccgtag acgtcagtca cttcagaaat actggtcatc
    22261 cgcccttctg gcaatgacgc catgtagatc agcgcacgta atccgtaatc agtgaaactc
    22321 gttaactgca catcaacctc aaaagggaaa tcgggaaaaa atacatttat attgatgata
    22381 aaccagccac aagctgtgtc gctaatttat ttcagttcgg ggaaggaaaa aagcgaggat
    22441 ttaacactgt gtcggatagc gtaatggctt aaccgacaca gcaatgtatc acactgttag
    22501 cccggcaggc aaaatatctg ccaggcgtac cagaattacg cgtcgaacgg gtcgcgcaga
    22561 atcatggttt cagtacgatc cggaccggta gagatgatat cgatcggtac accggtcagc
    22621 tcttcaatgc gcttgatgta gttcagcgcc gcctgcggca ggccgctacg atctttcacg
    22681 ccgaaggtgg attcagacca gcccggcatg gtttcgtaaa tcggctctac acctttccag
    22741 tcgtcagctg ccagcggagt ggtagtcact tcacgaccat ccggcatacg gtaagccaca
    22801 cagagtttca cctctttcag gccatccaga acgtccagtt tagtcaggca gaagccagac
    22861 agggagttca gctgtaccgc acgacgaacg gcaacggtgt ccagccagcc ggtacgacga
    22921 cgacgacccg tagttgcgcc gaattcgtta ccctgcttgc agaggaactc gccagtttca
    22981 tcaaacagtt cagtcgggaa cggacctgca cccacacgag tggagtaagc tttgaggata
    23041 cccagaacgt aatcaacata acgcgggccc aggccggaac cggtcgccac gccaccagca
    23101 gtggtgttgg aagaagttac gtacggataa gtaccgtggt cgatatccag cagcgtaccc
    23161 tgcgcacctt cgaacatgac gaaatcgcca cgctgacgcg cctggtcgag caggtcagaa
    23221 acgtcaacca ccatagaagt caggatgtcg gcaacagcca tcgtatcatc cagaactttc
    23281 tggtaatcaa ccgcttcagc tttgtagtag ttaaccaact ggaagttgtg atattccatc
    23341 acttctttca gtttttcagc gaaggtttct ttgtcgaaaa ggtcgccaac acgcagaccg
    23401 cgacgtgcca ctttatcttc ataagcaggc ccgataccac gaccggtggt gccgatcgct
    23461 ttcgcgccac gcgctttctc acgcgcgtta tccagcgcaa catgataatc aaggatcagc
    23521 ggacatgctt cagacagcag cagacgctca cgaacgggga tgccacggtc ttccagttct
    23581 ttcatctctt tcatcagcgc ggccggagac agcacaacac cgttaccgat gatgctagtt
    23641 acattctcgc ggagaatacc tgatggaata agatggagaa cggttttttc accgttgatt
    23701 acgagagtat ggcctgcgtt gtgaccgccc tggtagcgta caacatattt agcccgttca
    23761 gtcagaagat cgacgatctt acctttacct tcgtcacccc attgggtgcc cagtacgacg
    23821 acgttgttac ccatttttca aaatcaccgt ttgcttaaaa atggattcta ccatcgcttt
    23881 ttcagagtta cagcactttt tgcactcaaa aatgacctcg atcagtctgt tttttgttca
    23941 gccaatcgtt ttcctcaaca tgtagtagac cacaacgccc gcaaccacaa gtccaccgcc
    24001 aaaacgacgt aaaatattat cgggcaaatt ggtcatcgca gagatcatct tcttccatgc
    24061 cttcgggtaa agcatcggcc ctaaaccttc cagtaccaaa accagggcaa gcgccagcca
    24121 gattgtcgaa ttcattcatt atccttatag aaaaagaaaa ccaccgacat ccctgaggat
    24181 gcggtggctt tattgacctg taccgcagtc gttatattaa cgcgttgcgg aagtcggcgt
    24241 cttcatgtag cggaagaaat cgctatccgg gctcatgacc atcacgtcct gattgccaga
    24301 gaagctgttc tcataagcac gcaggctacg gatgaatgcg tagaagtccg gatctttact
    24361 gaatgcatca gcaaacagtt tggctgcttc ggcatcgcct tcaccgcgca tgatgcggcc
    24421 ctgacgctca gcttctgcca gcgttctggt cacttcatag tcggcagtcg cgcgcagttt
    24481 ttccgcttct tcctgacctt gtgaacggtg acgacgcgct accgcttcac gctcggcgcg
    24541 catacggttg tagatcgctt cagacacttc ggtcggcagg ttgatctgct taattcgcac
    24601 atcgacaact tcaataccca gcgccgccat actgttcggg ttgatgaccg gaactttgcc
    24661 cttcgtctca gccgttacgc gctctgccgc ttcggcaatg gcgttatctg ccgccggggt
    24721 agtaacttca tcttctgtac ccgcagaacc ggagttcagc gcgtcacgta cttcgagggt
    24781 cagacgacca cgggaatcgg tgacgatgtc tttcacgtcc aggcgaccaa tttcagaacg
    24841 cagacggtca gagaacttac gtttcaacag cacttccgct tgcgaaatgt cgcccccccc
    24901 cgttgccagg tagtaacggc tgaaatcgct gatgcgccat ttgatgtaag agtcgacgat
    24961 caggtctttc ttctctttgg tcacaaagcg gtcggcctgg ttgtccatgg tctgaatacg
    25021 tgcgtcgagc attttcaccg tttcaatgaa cggtatcttg aaatgcagac ccggctcata
    25081 aaccagaggt ttgttgtcat cgtcacgcag taccttacca aaacgcagcg taataccgcg
    25141 ctcaccttct ttgacgacaa agacagacat gtaaagcact accagcacga tgatgataat
    25201 cgcgataact gacttacgca tcgttattcc ccctgacgct ggtagtcgtt acgctgcgca
    25261 ttggcgcggc gttggtccat aatatcgccc tgactggtgg acgacgtgtt gcttgctcca
    25321 ctggttgtgg aggaagaggc tggcggcaga cgcagcagat tgctggcacc gttatcgctc
    25381 ttcgccgcag gggcgttacc acctttcagc atctggtcta acggcagaac catcaggttg
    25441 ccacctttat cgttaaccag cactttgcgg gtgttaccca acactttttc catcgtctcg
    25501 atatacagac gctcgcgagt aatttccggc gcggctttat attccggcag aagtttagca
    25561 aaacgcgcca cttcaccctg agcttccagg atggtctggg ccttgtacgc acgcgcctct
    25621 tcgaggatac gttgcgcctg accgttcgca cgcggctgaa cttcgttggt atacgcttct
    25681 gcttcacgaa tgtattgctg ttcgttttca cgcgcggcaa tcgcatcgtc aaacgccgct
    25741 tttacttctt ccggcggacg agcagcctgg aagttgacgt ccagcagcgt gatacccatg
    25801 tcatacggac gaatcgtctc ttccagttcg cgctgagtat cgctacgaat cacggtacga
    25861 ccttccgtca gaatgcggtc catggtgtat ttaccgataa ctccacgcag ggcgctgtcg
    25921 gtagcctgac gcaggctgtc atccgggctg gtcacgctat acagatattt ttccggattg
    25981 gtgacgcggt actgcacgtt catctcaacg cgcactacgt tctcgtccga cgtcagcatc
    26041 acaccagaag ctgccagttc acgcacggct tccacgttca ccggtttgac ttcgtcgata
    26101 aacgtcggtt tccagttcag acctggctca accagatggc tgaatttacc aaagcgtgtt
    26161 accacgccgc gttcggcttc tttaatggta tagaaaccac tggccgccca gataatgaca
    26221 atcgctgccg ctgcgatggt aacgacacga ccgccaagct gcgggcgcgg gccttgcgat
    26281 gaactgccac cgccagatcc ggtgccttta ccgccgccca gaccaccgag ctttttgctc
    26341 agtttgcgga agatatcatc taaatcaggt ggcccttgat cgcgaccgcc tttgtttcca
    26401 tttccctcag agttgccgcc aggtttgctg cttccccacg ggtcgcggtc ttgtccgtta
    26461 ttaccgggct gattccacgc catgtttgtg ctccatattt gttatgcggt gatccctgtt
    26521 gtctttcgac gccagaggat atgactccac gcttcagacg ctacgccgtt agatcaggta
    26581 atcgatcaac gccggttctt gtttacagag gcgacgccag tcaacgatcg gcatacgaac
    26641 ttgcagactt acgctgccgt cctcctccat ccactctttt tctattgcct gaagctgata
    26701 aaaacgactt ctcagacgcc cttcctgcgg tggcagacgc aatgtatgct gcgccacctc
    26761 gccggaaagc cgctccgtca aagcctgaaa aagctgtggt atccccgctc cggtctgtgc
    26821 ggaaagccag acacggatcg gtttgttctc ttcgtcccga tcaatacgcg gttcgaaatc
    26881 ttccagcata tcgatcttgt tcatcaccag cagggttggg atctcgtgag cgtcgatctc
    26941 ttcaagaacc gtattcaccg cttcgatgtt ttcttgtaca cgcacatccg ccgcatcaat
    27001 cacatgcagc agcaatgtgg cttgccgcgt ctcttgtaac gtggctttaa atgctgccac
    27061 cagatcgtgc ggcaggtggc gaataaaccc tacggtatct gcaagtacgg tttcaccgac
    27121 atctgcaacg tcaatacgcc gcaacgtcgg gtcgagggtg gcaaacaact ggtctgccgc
    27181 gtagacccgc gcttcggtga tgcgattgaa aagggtagat ttaccggcgt tggtatatcc
    27241 caccagcgaa acagtaggaa cgtcggcttt gatacgcgat tgccgcccct gctcacgctg
    27301 cttttcaact ctttccaggc gcgactgtat ctgcacgatg cgattacgca acaaacgacg
    27361 gtcggtttcg agctgggttt cacccggacc acgcaaacca atcccgcctt tctgtctttc
    27421 aaggtgggtc cagccacgca ccaggcgtgt agccagatgg cgcagctgcg ccagctcaac
    27481 ctgcaactta ccctcatggg tacgcgcacg ttgggcgaaa atatctaaaa taaggccggt
    27541 gcggtcgata acacgacact cgcacaaacg ctccaggtta cgctcttgcg ccgggctcag
    27601 ggcatggtca aaaagaacga ccgaagcacc cgtcgctttg acagcttccg caatttcaac
    27661 tgctttacct tcacctacaa aatactttgg gtgcggcgct ttacggctac cggtaatcac
    27721 ctgcaatgct tcgacaccgg cggaagagac cagagattca aactcctgca ggtcttccat
    27781 atctttgtct tgcgtaaaat agatgtgtac cagtaccgcc tgctcaccag catcataacg
    27841 gtcaaacaag cgtataaccc tctaaataga tcagcgggga acgcaggatc gctggctccc
    27901 cgtgtaaaaa aaacagcccg aaaccttatt cggtttcttc gctgtcctgt tgcgcggaag
    27961 tattctgcgc gctgctacca tgatggtagt tactgctggt accgccaccg gcgttgttac
    28021 tgtgatgaga aaccgggcga gacgggacaa cagtagaaat cgcgtgcttg taaaccatct
    28081 ggctgaccgt gtttttcaac aggatcacga actgatcaaa agactcgatt tgcccttgca
    28141 gcttaatacc attcaccaaa taaatagaaa ctggaacacg ttcccgacgc agtgcgttca
    28201 ggaacggatc ttgtaaagat tgccccttag ccattctctc ttttccttat atgcttattt
    28261 gtactttgaa cctttcgatt ctgaaaaaat tgcgcacgat acgtctcaat tgtacacatt
    28321 cagcctgcga tagcaccaac aacctgtaat acttcgtcac gcgcctgttc tggtttttca
    28381 ctgtcaagcc agtgaacccc ttcccaacca cgcagccagg ttatctgccg cttcgccaac
    28441 tgtctcgtgg cgcaaacacc tcgataaacc atttcatcgt atgagatttc gcctctaagg
    28501 taagaccaca tctggcgata acccacgcaa cgaatggaag gcaagtccgt atgcaaatct
    28561 cctcgggcaa aaagcgcccg gacttctgct tcaaaacctg aagccaacat ctgatgaaaa
    28621 cgctgctcaa ttcgttgatg gagcagttca cggctcgccg gggcgatggc gaactgatgc
    28681 acctgatacg gtagagcgtc tcctgacgtt tgcgtcagtt ccgttaaagt tttacccgaa
    28741 atgaaaaaaa cttccagtgc ccgggaaagc ctttgtggat catttggatg aatccttgct
    28801 gccgcaaccg gatcgatctc ctgaagttga cgatgcaatg actcccaacc ttgctctgcc
    28861 gcctgttgct caattctggc ccgtacttcc gggtctgccg acggtagcgg cgacaaccct
    28921 tccagcaatg ccttgaaata caacatcgta ccgcccacta acagtgggat ccgccccgcc
    28981 gcggtgatat cggccatttc cgccagcgca tcgcggcgaa aatcagcagc cgagtaagcc
    29041 tgcgacggat cgcgaatatc cagcaatcgg tgcggcgcgg cgagtaactc ttcagcgttc
    29101 ggcttcgccg tcccgatatc catccctttg taaataaggg cagaatcaac gcttatcaac
    29161 tctactggta aaattttacg cagctcaatg gctaacgccg ttttaccgga ggccgtcggc
    29221 cccatcaaaa aaatcgcctt aggcaggctc gccttactga tatcactcat ctttcagggc
    29281 ttttatcgcc ggatgtaaat caacagattg taacagacca cccggcggcg ttttcacaag
    29341 ttgcggacat aaccgttcca cgtccgccag cagggttatg gcctgtgcca ttgaccactg
    29401 cgcatgttcg ctcatcagat ttcgtgcaat ccactgcgca atattgccag gttcgaatac
    29461 ggactgcttc gctaggtagc ctatcagttc aggaatcaag atttgtaaat tttgttggcg
    29521 taagggtaaa ggcacggccc tgatggtcac atgctgtgca tctgactgga aatcaatacc
    29581 caattccgcc agggcagact gcgctttttc taatgccgat ttttcttcgc cagaaacttt
    29641 tagccgcaac ggaatcagca acggctgggc gcaaacgggc gcttcacccg gcgtcagttg
    29701 tacctgacgc agccaacgtt ctgccactgg caaggctaaa agtgaaatgt tgccgtcgcg
    29761 ctccagcaac gcacagtcgg aatggacgat agtcagtacc cgaccaaaac tctgactgtt
    29821 cgccgcaagt gcaggttcct gcggttccgg cgcttttggt ttttgcatcg gcgcgggcgt
    29881 ttgcaaaagc tggcgataca cttcaccttg ctgtttctgg taacctggct gcgcattcgg
    29941 ccagggggca gccggacgac tacctgatgc tggcgcagga gtgtagcgcg gagctactgg
    30001 ctcacgaact gccggttcag caaagtgatt gcgccccgcc gccacgcggt tttccggaat
    30061 gggacgcggt gcaggttggg gttcatcgtc cagcggtagc ggcgtttcca gttgctgttg
    30121 tagcacgctc agcacgccct gatagataaa gtcatgcacc agacgcgact gatgaaaacg
    30181 cacttcgtgt ttggcggggt gcacgttgac gtccacctga tgcgggtcga tctccagata
    30241 caacacaaat gcaggttgct gatcggcccc cagtttgtct tcacaggcct ggcggatcgc
    30301 gtgattgatc aggcgatcgc gcatcatgcg accattcacg tagcaatact gaatttccgc
    30361 cagtgcgggc gtggtgtgat ttggatcggc cacccagccg cgcagcgtga gatcgccgtg
    30421 ttgccattca atcgccagcg cttgttcgag aaaagcggtg ccacaaatcg cgcctaagcg
    30481 ccgttctttt tgcccgcctt ccggcaccgc gcggtactgg cgtacgattt tgccgttatg
    30541 cgacaggttg atcgtgacgt cgaaacgcgc cagtgcgatg cggcggatga tttcatcgat
    30601 atggttaaat tcggttttct cggtgcgcag gaatttgcgc cgcgccgggg tgttgtagaa
    30661 caggtccagt acctccagcg tcgttcccac cggatgcgcc gccggtttaa ccgtcacgtc
    30721 catatcgcgc ccttcggcat aggcctgcca ggcttcctgc tgttctgcgg tgcgtgaagt
    30781 gagcgtcagg cgggaaaccg aactgatact cgccagcgcc tcaccgcgaa agcccaggct
    30841 gataatggct tcgagatcgt ccagagaggc gattttactg gtggcatgac gcgccagcgc
    30901 cagcgccagc tcgtcttttt tgataccaca gccgttatca cgaatgcgga taagtttcgc
    30961 cccaccgcgt tcgatatcaa tatcgatacg cgtcgcacct gcatcgaggc tgttttccac
    31021 cagttctttg actaccgacg caggtcgctc gaccacctca cctgcggcaa tctggttcgc
    31081 cagttgtggc ggtaagacct gaattggcat caatcgtcct tagtttggca gcgtgcgatc
    31141 tggcgtcgtc accgtactgg cagtttgtgc cgttgcaccc tgcggcgcag attgcatcgg
    31201 atgcgcaagg aaataattgc gcaggccttt gtaaatggct tctgccagct gttgttggta
    31261 atcgtcgctc gccagcaaac gttcttcgct gttgttgctg ataaaaccgg tttcgaccag
    31321 tactgatggg atatccggcg agcgcagaac gccaaggctg gcgtgttctg gtcgacgttt
    31381 atgtatttcg ccaatgcgtt gcaactgact gatcatactg gtcgctacat cataccctac
    31441 ccgctgggaa tgaccgaact gtaaatccag caccgcctgg cttaaatagg ggtcagactg
    31501 actgttcgcc agcacatcac ccgccccgcc cagcagctcc gactgtttct cgtgctgctc
    31561 cagccagctg gccatttcgc tgttggcgcg acggttagag agcacccata cggaagcgcc
    31621 agtcgcactg cggttcggtg cggcatcagc gtgaatcgac acgaggaaat tggcgttttg
    31681 cttacgtgcc acatcgctgc gccccatcac cgagataaag taatccccgt cacgggttaa
    31741 aacgccttta aacatcggat cgtcattgag caaagtacgc aatttacgcg cgatggcgat
    31801 ggtgacattt ttctcccgcg taccaccggg gccgatagcg ccagggtcct gaccgccgtg
    31861 tccggcatca atagcgatga taattttatc gccattgtta gccgtcgcgc gcgctgccgg
    31921 acgcgttacc gtattactgc tgataacacc cgtagtgcgg ttactttccg ttttaaacgg
    31981 attgcgcgcc ggttcgctga cgcgcggtgc gacaaccgta ggcgtttcaa cgcgtttcac
    32041 aaccacaggc ggcggaggag gcggtggcgg cgcatcggcg ttaatcgtaa agacgacagt
    32101 gtaattgctg ccattctgcc gcttcaccgc ttcggtttta ccattttcgg taagatcgac
    32161 caccagccgt agcgtttgtg catctttagg cgttccggag cgaatcgcct tcaccagatt
    32221 attaccgctg aacaacaacg gcagtccctg aatcacgccc gtttgtttga tatcgagcgc
    32281 cacggtgcgt ttgctttgat ggctaaacgc ataatcagga tcgccaataa aactcaacgt
    32341 tatccgcgcc tgttggttgc cgttagaaac ctgaatatca gagagcgtcg cggcacccac
    32401 ctgcgcgcac agcagcagca acgtcgctac caaccaattt ctgatgcgat acatcatccc
    32461 accacctttc aaaggttaac cggctaaacg tgccagcaac aattcacccg cagaggaaac
    32521 cgcactcacg cgcgcctcac ggccttgtgc ctgataatca atgtgtattt cgacatccgg
    32581 gtcaggaaga acacctgtac cttgttgtgg ccactccacc aggcagatgg catcgttggc
    32641 aaaataatcg cggatcccca taaactccag ctcctcggga tcggcaaggc ggtacaaatc
    32701 aaagtgataa accattaaat tgtcgagcgt atagggttcg accagcgtat aagtggggct
    32761 tttgacatta ccctgatggc ccaaagcctg taaaaagccc cggctaaagg tggttttacc
    32821 tgcgcctaaa tcgccataca gatagattac ggttgcgcca tcgcaggctt tcgctacccg
    32881 ctcgcccagg tctaatgttg cctgctcatc agggagcgga attactcgat tcatcatggt
    32941 ttttatcagt cacttccggg ttaacaatac gctgtagcgt ggaaaagaga tcggttgcca
    33001 gcatcccgcg cgttccaaaa cgcgccgcca gtacgtcagc tgccgcaccg tgcgcgacac
    33061 agcctgcaca ggctgcatca tacggcgaca gtttttgccc aagcaatgcg ccaataatac
    33121 cagagagcac atcgcccatg ccgccgcttg ccatgcctgc atttccggca tcaataatgc
    33181 ctaaagcgtc aggatgggcg gcgaccacgg ttccggcacc tttcagcacc gctacgccgc
    33241 cataacgttg taccagacgt ttggcgcaat gtaagcggtc actttcaatt tcagcgacgg
    33301 aacagcctaa caaccgtgcg gcctcgccag gatgcggcgt gatcacgcga ttgtgacgct
    33361 tatcgggatt gattgccagc aggttcaatg catcggcatc ccacaacatc ggtttgcgaa
    33421 aattctcaac tttttgcagg gcttttttcc cccactcttg ctggcccaga ccgggaccaa
    33481 tcaccaccac atcggcccat tccaggcttt cggtaagaga gtccatagtc agttcatgca
    33541 ccatcaattc tggtcgtgca gtcagcagcg gcgcaatgtt ctcactgcgg gtcagtactc
    33601 ggactaaacc agcaccagca cgtagcgccg cttcccccgt catacgaata gcccccgccg
    33661 tgccgtgatc gccaccgata atcaccagcc gcccgtgatc gcctttatgc gaagtcgggc
    33721 gacgcggttt cagccagtga gaaagttgtt ctgccgaaaa ccgctgaatt ttcgtctcct
    33781 gacctgccag ccagctatcc agccccagtg agtcaaaatg cagttgtcct gtaacatccc
    33841 gcgcttttcc agtgagcaag cctggtttca gcgcaataaa agtgatggtg tgatcggcgt
    33901 tgatcactgc gcctggcgta gcgccggttt cagccagcag gccggaaggg atatcaaccg
    33961 ccacaatcgg cgcaggatgg gaattagcgt ggtcgattaa ctggctaatg gattcgcggg
    34021 gtgcttgctg caagccggta ccgagcagcg catcaacaat cagagctacc gactcgggcc
    34081 agacaatatt cgaagcatgg atctcgcctc ccgcgtttaa ccatgcctcg cgtgccagcg
    34141 cggcctcttc cggcaacggt ttatcgctct cctgggccaa caacgtgacc tcaatgccga
    34201 ccgctttggc cagtcgcgcg accacgtagc catcgccgcc gttattacca tgaccgcaca
    34261 acaccagcca gtggcgggcg tcaggatacg ccgaacgaca cacctggaat gcggcctcgc
    34321 cagcgcgaag catcagctca tagagtgtga gccccagcgc atctgccgcc tcgcgttctc
    34381 cgcggcggat atcgtcggcg tgccagacgg tgtgtggtat acttacgggg tttttcttca
    34441 ttgtatggtc cgtcatgtca gagcccctcg atctcaatca gttagcgcaa aaaattaaac
    34501 agtgggggct ggaactgggc tttcagcagg taggtattac cgataccgat ctcagcgagt
    34561 ccgagcccaa actgcaagca tggctggaca aacaatacca cggcgaaatg gactggatgg
    34621 cacgtcacgg tatgctgcgc gctcgccccc atgagttatt gcccggtacg ctgcgcgtga
    34681 tcagcgtgcg gatgaattac cttcctgcta acgccgcatt tgccagcacg ctgaaaaacc
    34741 ccaaactcgg ctatgttagc cgttatgcgc tgggccgtga ctatcacaaa cttctgcgca
    34801 accgactcaa aaagctgggc gagatgattc agcaacattg tgtttcgctg aattttagac
    34861 cgtttgtcga ttctgcgcct attctcgagc gcccgttagc tgaaaaagct gggctcggct
    34921 ggacaggtaa gcactcactt atcctcaatc gcgaggccgg ttcgttcttc tttttaggcg
    34981 aattgctggt cgatattccg ctgcccgtgg atcaaccagt cgaggaagga tgcggcaaat
    35041 gcgtggcctg tatgacgatt tgcccgaccg gtgccatcgt cgagccatat accgtcgatg
    35101 ctcgccgctg tatctcttat ctcaccatcg aactggaagg ggcgatcccg gaagagttgc
    35161 gaccgttaat gggaaaccgt atttacggtt gcgatgactg ccagcttatc tgcccgtgga
    35221 atcgctattc acaactcact acagaagacg atttcagccc gcgtaagccg ctacacgcac
    35281 cggaactcat tgagttattc gcctggagcg aagagaagtt tttaaaagtc acggaaggtt
    35341 cggcgattcg ccgtataggt cacctgcgct ggctgcgtaa tatcgccgtg gcattaggca
    35401 atgcgccctg ggatgaaacg attctggcgg cgctcgagag tcgcaaaggt gagcacccac
    35461 ttcttgatga gcacatagcg tgggcgatgg cgcagcaaat cgagaggcga aatgcgtgca
    35521 tagtcgaagt gcaattgccg aaaaaacagc gtctggttcg ggtaattgaa aaagggttac
    35581 cgcgtgacgc ctgattcatc cacagcttgt gaataaaaat aaaaacgcat tgcaattcaa
    35641 gacgaataaa atcgtcaagt gatcgactcg acaaaacgaa atcttaattt ttaaattatc
    35701 tatcaatagg ttaaaaaaat actattcacc gtgcgaagtt tctttgaaat taagattacg
    35761 gcctgaggct gtggataact ctgtttacaa gagtttgtca gagacaacaa aaaccagcaa
    35821 taacgcatcg cgtcgctgtg gatattttat tgagagaaga atttgg
//
LOCUS       DAZFBR010000039        35733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFBR010000039 DAZFBR010000000
VERSION     DAZFBR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.039"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(13..88)
                     /locus_tag="ACHJZ2_002146"
     tRNA            complement(13..88)
                     /locus_tag="ACHJZ2_002146"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53..55),aa:His,seq:gtg)
     gene            complement(147..223)
                     /locus_tag="ACHJZ2_002147"
     tRNA            complement(147..223)
                     /locus_tag="ACHJZ2_002147"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(187..189),aa:Arg,seq:ccg)
     gene            complement(326..1711)
                     /gene="yifK"
                     /locus_tag="ACHJZ2_002148"
     CDS             complement(326..1711)
                     /gene="yifK"
                     /locus_tag="ACHJZ2_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4568251.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1902..2642)
                     /gene="wecG"
                     /locus_tag="ACHJZ2_002149"
     CDS             complement(1902..2642)
                     /gene="wecG"
                     /locus_tag="ACHJZ2_002149"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO4568252.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2645..3997)
                     /gene="wzyE"
                     /locus_tag="ACHJZ2_002150"
     CDS             complement(2645..3997)
                     /gene="wzyE"
                     /locus_tag="ACHJZ2_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO4568253.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3994..5073)
                     /gene="rffT"
                     /locus_tag="ACHJZ2_002151"
                     /gene_synonym="wecF"
     CDS             complement(3994..5073)
                     /gene="rffT"
                     /locus_tag="ACHJZ2_002151"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO4568254.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5070..6320)
                     /gene="wzxE"
                     /locus_tag="ACHJZ2_002152"
     CDS             complement(5070..6320)
                     /gene="wzxE"
                     /locus_tag="ACHJZ2_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO4568255.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6322..7452)
                     /gene="rffA"
                     /locus_tag="ACHJZ2_002153"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6322..7452)
                     /gene="rffA"
                     /locus_tag="ACHJZ2_002153"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO4568256.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7457..8131)
                     /gene="rffC"
                     /locus_tag="ACHJZ2_002154"
                     /gene_synonym="wecD"
     CDS             complement(7457..8131)
                     /gene="rffC"
                     /locus_tag="ACHJZ2_002154"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO4568257.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8109..8990)
                     /gene="rfbA"
                     /locus_tag="ACHJZ2_002155"
     CDS             complement(8109..8990)
                     /gene="rfbA"
                     /locus_tag="ACHJZ2_002155"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO4568258.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9009..10076)
                     /gene="rffG"
                     /locus_tag="ACHJZ2_002156"
     CDS             complement(9009..10076)
                     /gene="rffG"
                     /locus_tag="ACHJZ2_002156"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO4568259.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(10076..11338)
                     /gene="wecC"
                     /locus_tag="ACHJZ2_002157"
     CDS             complement(10076..11338)
                     /gene="wecC"
                     /locus_tag="ACHJZ2_002157"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO4568260.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11335..12465)
                     /gene="wecB"
                     /locus_tag="ACHJZ2_002158"
     CDS             complement(11335..12465)
                     /gene="wecB"
                     /locus_tag="ACHJZ2_002158"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO4568261.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12521..13567)
                     /gene="wzzE"
                     /locus_tag="ACHJZ2_002159"
     CDS             complement(12521..13567)
                     /gene="wzzE"
                     /locus_tag="ACHJZ2_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO4568262.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13579..14682)
                     /gene="wecA"
                     /locus_tag="ACHJZ2_002160"
     CDS             complement(13579..14682)
                     /gene="wecA"
                     /locus_tag="ACHJZ2_002160"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO4568263.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14922..16181)
                     /gene="rho"
                     /locus_tag="ACHJZ2_002161"
     CDS             complement(14922..16181)
                     /gene="rho"
                     /locus_tag="ACHJZ2_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO4568264.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16266..16367)
                     /gene="rhoL"
                     /locus_tag="ACHJZ2_002162"
     CDS             complement(16266..16367)
                     /gene="rhoL"
                     /locus_tag="ACHJZ2_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO4568265.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16508..16837)
                     /gene="trxA"
                     /locus_tag="ACHJZ2_002163"
     CDS             complement(16508..16837)
                     /gene="trxA"
                     /locus_tag="ACHJZ2_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO4568266.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16968..18233
                     /gene="rhlB"
                     /locus_tag="ACHJZ2_002164"
     CDS             16968..18233
                     /gene="rhlB"
                     /locus_tag="ACHJZ2_002164"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO4568267.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18369..19853
                     /gene="gppA"
                     /locus_tag="ACHJZ2_002165"
     CDS             18369..19853
                     /gene="gppA"
                     /locus_tag="ACHJZ2_002165"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO4568268.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19900..21921)
                     /gene="rep"
                     /locus_tag="ACHJZ2_002166"
     CDS             complement(19900..21921)
                     /gene="rep"
                     /locus_tag="ACHJZ2_002166"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO4568269.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22138..22587
                     /locus_tag="ACHJZ2_002167"
     CDS             22138..22587
                     /locus_tag="ACHJZ2_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO4568270.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            22786..23067
                     /gene="ppiC"
                     /locus_tag="ACHJZ2_002168"
     CDS             22786..23067
                     /gene="ppiC"
                     /locus_tag="ACHJZ2_002168"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO4568271.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23113..24588)
                     /gene="ilvC"
                     /locus_tag="ACHJZ2_002169"
     CDS             complement(23113..24588)
                     /gene="ilvC"
                     /locus_tag="ACHJZ2_002169"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO4568272.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24738..25631
                     /gene="ilvY"
                     /locus_tag="ACHJZ2_002170"
     CDS             24738..25631
                     /gene="ilvY"
                     /locus_tag="ACHJZ2_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO4568273.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(25683..27227)
                     /gene="ilvA"
                     /locus_tag="ACHJZ2_002171"
     CDS             complement(25683..27227)
                     /gene="ilvA"
                     /locus_tag="ACHJZ2_002171"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO4568274.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27230..29080)
                     /gene="ilvD"
                     /locus_tag="ACHJZ2_002172"
     CDS             complement(27230..29080)
                     /gene="ilvD"
                     /locus_tag="ACHJZ2_002172"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO4568275.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29145..30074)
                     /gene="ilvE"
                     /locus_tag="ACHJZ2_002173"
     CDS             complement(29145..30074)
                     /gene="ilvE"
                     /locus_tag="ACHJZ2_002173"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO4568276.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30094..30357)
                     /gene="ilvM"
                     /locus_tag="ACHJZ2_002174"
     CDS             complement(30094..30357)
                     /gene="ilvM"
                     /locus_tag="ACHJZ2_002174"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO4568277.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30354..32000)
                     /gene="ilvG"
                     /locus_tag="ACHJZ2_002175"
     CDS             complement(30354..32000)
                     /gene="ilvG"
                     /locus_tag="ACHJZ2_002175"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO4568278.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32003..32053)
                     /gene="ilvX"
                     /locus_tag="ACHJZ2_002176"
     CDS             complement(32003..32053)
                     /gene="ilvX"
                     /locus_tag="ACHJZ2_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO4568279.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(32140..32238)
                     /gene="ilvL"
                     /locus_tag="ACHJZ2_002177"
     CDS             complement(32140..32238)
                     /gene="ilvL"
                     /locus_tag="ACHJZ2_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO4568280.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            32591..34111
                     /gene="yifB"
                     /locus_tag="ACHJZ2_002178"
     CDS             32591..34111
                     /gene="yifB"
                     /locus_tag="ACHJZ2_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO4568281.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(34136..34474)
                     /gene="maoP"
                     /locus_tag="ACHJZ2_002179"
     CDS             complement(34136..34474)
                     /gene="maoP"
                     /locus_tag="ACHJZ2_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO4568282.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34593..35432
                     /gene="hdfR"
                     /locus_tag="ACHJZ2_002180"
     CDS             34593..35432
                     /gene="hdfR"
                     /locus_tag="ACHJZ2_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO4568283.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(35528..35603)
                     /locus_tag="ACHJZ2_002181"
     tRNA            complement(35528..35603)
                     /locus_tag="ACHJZ2_002181"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35568..35570),aa:Trp,seq:cca)
     gene            complement(35612..35688)
                     /locus_tag="ACHJZ2_002182"
     tRNA            complement(35612..35688)
                     /locus_tag="ACHJZ2_002182"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35652..35654),aa:Asp,seq:gtc)
BASE COUNT         8722 a         9749 c         9023 g         8239 t
ORIGIN      
        1 aacttctaat aatggggtgg ctaatgggat tcgaacccac gacaactgga atcacaatcc
       61 agggctctac caactgagct atagccacca ctacaaatct tgttacgcgg tattactacc
      121 accgcagctc aagcgccggg actaaatggc gcgcccgaca ggattcgaac ctgagacctc
      181 tgcctccgga gggcagcgct ctatccagct gagctacggg cgcttagcgc cgttgcgggg
      241 gtggataata cggacttccc ccccctctgt ctagtccctt tttaaataaa ttgcgcgttt
      301 ggttacggtt tgtgcgtttt gctgcttatt cctccagttt atgcgctttc ccgtggcgat
      361 taaggccaaa aactttataa atcgccgtca ccgccagcat aaagatgatg ccaacaaaca
      421 gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa aacgcaaatc aggaatgcca
      481 ttgttacgta gttggcccac gggaacagga ttgagcggaa cggatggctg gcaatcgccg
      541 ctttatgtgc acgccgaaaa cgcagctggc ttatcaatat cacaaaccat ggcaccatcc
      601 ccggaagcac gctggcactg tagacgtaga caaacacacg ctgcggattg ggaatgatgt
      661 agttcaggca tgagccaatt agcagaatag caatagatac tgccacaccc gcaaccggta
      721 cgccgtgacg ggaaactttc gccatcgccg ccggtaactg acggtttttc gccagtgcgt
      781 agagcatacg tccgcaactg tacatgccgc tgttacagcc agagagcgca gccgtcagca
      841 ccacaaagtt gataatgccc gccgctgcgg taataccgat tttggcaaaa gtcagtacga
      901 acgggctgcc gttgctgcct atttcattcc acgggaagat ggtgacgata acgaaaatcg
      961 cgccaacgta gaaaatcagg atccgccaca gcaccttgcc tacggcactg cgcagcgtca
     1021 cctgcggatt cttcgcttca ccggcagtaa tgccaatcag ctccacgccc tggtaggacg
     1081 ccaccacaat acacagcgcg gtcaggaacc ctttccagcc acccgcaaag aaaccgccat
     1141 gctctgtgag attgctaaaa ccaatcgact gcccgccatt gccaaagcca aagaaaatca
     1201 cgcccaggcc aatgacaatc atcacgataa tcgtggtgac tttgatcatc gcgaaccaga
     1261 actcgatttc gccgtacaac cgcaccgctg ccagattcgc caacgccacc agcgccactg
     1321 cgatcaatgc gggtatccac tgcgccatct ccgggaacca gaactggaca taaacgccaa
     1381 tggcggtgat ttcagagatc cccaccgcca tccacataaa ccagtaagac caggcggtga
     1441 gatagccaaa gaacgggctc atataacgat gcgcataaac ggcgaacgaa ccggtaaccg
     1501 gttcgaggaa caacatttcg cccattgaac gcatgatgaa aaagacgaac agcccggcga
     1561 tgatataggc caacaatacg gatggcccgg cccatttcag ggtactggcg gcccccataa
     1621 acaggccgac gccaatggtg ccccccaggg cgatgagttc gatatgtcga gcttccagcc
     1681 cacgctgtag ctctggtttg ttatctgcca taaatcctcg tgttgtgttt gcatgctttc
     1741 cggtgttacc ggttatcgtt atgggtacat cgagtgttgc gaatgttttc gtaattcagg
     1801 agaaatggca aataaagcat taaaaatttg aatgctttgt gtaataaaaa agcagacagg
     1861 cgacggagtg accactccgt cgctttacaa agagaggaaa atcagaggtt gccggtgtaa
     1921 tgccagcgta aataacgcag caaacgaagc tgacgcttaa tgcggctcgg ctgcgaaagc
     1981 aggcggtaga gccactccag ccccagcgtt tgccagattt tcggtgcgcg ttttacgtga
     2041 ccggtgaaaa catcgtaagt cccgccaacg cccatataca gcgcatctgg atgcaccaga
     2101 cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca tcgcaacggt gacgatttgc
     2161 gctccgctgg catgaatgcg ttcaaacagc gcctgacgct gctcgggttt aaaataacca
     2221 tcctgactgc caacgatatt cacattccac tggttgcgca gtttagcttc agtttgcgcc
     2281 agcacttcag gtttaccgcc cacaagaaat accgtcgtcc cttctttgcc tgcgcgcgcc
     2341 atcagctctt cccagagatc ggcaccggca acgcgggaaa cctgcgcctg cgggtacttt
     2401 ttacgtactg aacgtacaac gctgatgcca tccgcatatt taaattcggc agcgttaatt
     2461 aactccctga cctcggcgtt ctcttcaatg gtcagcattt tttcagcatt aatggcaacc
     2521 agcgttccct gcttaagctg cccgtcagca aacagataat cgagggcatg ctgcatatca
     2581 cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg gtgccgtggt gttgttattc
     2641 attgttatcc ttcaacctgc gtccggagcg atgattttgt acgtttatga atgagtccgg
     2701 cgctttcaaa aagccagtac aacagttttg cgatcatcag acatgcgccg aagaccacga
     2761 taaaaaagac cacgcgtgag acaaacgaat ccagcccttc acgcgccagc acgatcatat
     2821 tgaagatcgc cccaaagcag aaactgtgca atatcgcagc cttatagcga ttagtttcgc
     2881 ggttgcccag ctcatacagc cagtcgaacc atttgatgat cagtccaacc acaatcgccc
     2941 cgagcgggat gaacaacgcg ccgcccatca ccaccagtga gcctataagc gtaggcgaga
     3001 tcgccagtcc ggagtggtta ttcagcactt cccaggtaaa gtagttggct gagttcagca
     3061 ccatactcgg gcgacccggc cacagccagg aagggataaa gacatagaaa tcgcggacaa
     3121 ttggcgccag gccctggaag tcgatgttgt cgtagttctg caacagcaac gccagattct
     3181 cccacggcga gaaggtgtcg cgagtgagat agagaaacgt atagaacgct tcatcgccgc
     3241 tcacattcat tccatagcgt tttagcgcca gccagaacat gccaacaatc cccagcacgc
     3301 ccgccgccgc cagcatccac aacgaaatcc agccgcgaat aatgccaata aacaggaaga
     3361 tagcgaatgc gatgatgata ttggcgcgag tgccgccgac aatcatataa gtcagcaagc
     3421 caaaggcgac cgtgctgacg aggaaaaaca gccacgcttt gctgtcctgg cgcagaaagt
     3481 agaccaccag catcgccggg atgaaaaagt aaaagaagcg ttttaacgcc acgccggaga
     3541 cttcactgga aaagatctga ctgtaggagt taagccggaa cagcaaaaag ccgttgtgca
     3601 tgaagaagat gccgacgctt accagcgcga tacccatcag gatcacccac gtaagattgg
     3661 tttccacgcg gttcatggta aacagcggac ggcgcggtac atcagcaacg cgtttgcgta
     3721 ggcgggtttt gtaggtgacg tagtaaaccg cgtagaagca gcccgcagaa agcaacgcct
     3781 gcaacaggat ttctggcggc gcgacaccaa catcaaagcg aaacaccagc acgctggtca
     3841 gcgggaagcc aaagaaaaag gtgagcagaa acagcaatga aaagaataca ttgaagttaa
     3901 agcgcacacg acgaaactca aaccaggtca gcgtggcaat aaacagcgtg cagagcagcc
     3961 agacaacaaa caggccactg aattgcagca gactcatgcg acctccccgg cggcaatcgc
     4021 caacgcccgc tgccagcctt gtagatagtt agggctaaag aaggcaatgg tgtttttatc
     4081 caccgacgcc aactggcgct gcgcttcacg cacaatatcc tcgttgagat cgtcggtagt
     4141 aaacagcacc ggtaaatgct gttccgtcat atcctgccag aacggatttt cccggttaag
     4201 cacacaagga atgcccgcct gaatcagtaa gcacagcgta ccaatgccct gctggcgggc
     4261 aaaaataaag taaccaagat cgcactgacg aagtagcgtc agataggcgt caaattccag
     4321 tttttcactc agaacttgca gattttcttc gctgaataac tccagccccg cctgacgaac
     4381 ttcctcgatg tacgcttcgt tatgaggcgg atatcccatc ggcaccacca cttttaccgt
     4441 atcgccaaat tgctgatgaa cggcgcgcaa ggcagcaata tgctcattgc tgcggtcgcc
     4501 ggagttcccc accagaatgg tcattttccc ttcacgttgc cgatcgttcg ccatcgtatt
     4561 gagagaagca tccatccgcg tcgggaagta cagcagttcg ccccgcacct ttgggtgcgt
     4621 tttggcaaaa aagctcaaat caccacgggt ggcaaataca cagccgactc gcttttgcgc
     4681 caggcgacgt agtgggtaaa aaagcttata tctcaagcca ctggaaagct cgtacaggtc
     4741 tgccccccag atatgccagt aaaactggct gggcttaatg ccaccactca gcagagccag
     4801 ccacagtgtg ggattgaact gaccgtggaa gaaaaaacgc tgctgacggt tagcttttgc
     4861 tttcgcgatg accgcttccg ccagcgattt tttcccaggg aaaaattgca cagaaagcgc
     4921 cggacagcta tcacttaagc cgtcgtcctt gccaacaacc ataaactcgc gcgcgtgctc
     4981 gctcgtcgcg gccagcgcgt cattgaaaaa ccgcaaaacg gttcggttat ggtgagggat
     5041 atccgatccc agtacgtgaa tcagtacagt catgcccgcc tacgccagag taaaaacacg
     5101 ccacaacaaa gagaaaaata gacgatatag gttgccatat atgcctgtgc cgcgcccagc
     5161 gcgccgtgcg tagggattag ccagtgggca aataccatca ataaagtgaa ctggctgact
     5221 tccgccagaa tataaaaccg cagtgaagct ttggcgatca ccagataacc aaagacataa
     5281 gcgcccactt ttaacacatc acccaccaac tgccaggcaa agagatcgcg catagcggta
     5341 aatttattcg acaacagcag ccagatagca aaatcacgca gcaaccagac ggtaaaactc
     5401 gccgccgcca ctgccggtaa gacgaatttc agcgatttaa ccacttcccg ggtgatatcg
     5461 cgcttttccg ttagccgcga caacgtgggc agcaaatata cgctgaacga tgccgtaata
     5521 aattgcaggt aggcatcgga aatactgctc accccttgcc agatccccac ctcatcccag
     5581 ctatactgcg ccgccagcag tttacgcatc atgatgtaag caacaggcaa ggtcaccgac
     5641 gtaatcaacg tcatgagcgt aaatttgctc aactgccctg ccagaccgtt atcccagctg
     5701 ggtttcagat agcttaacgg gatagcacca cgcttaatta acatgacggc ggcaggaatc
     5761 accaccagcg cgggaatcaa cgccagaccc agcaacgccc cttcatatcc gcccaaacgg
     5821 taactgacgt agtacgcaag aacgccaatc aagctgccaa caatcagaga taacgcatta
     5881 cctgcggcat cgcgaaagcc tttcatcaac gccagtaaca ggttgcccca ggcgatcccc
     5941 atttgcacca gcgccactaa acgcaccagc ccctgatagt cgtgattacc aaacaatccc
     6001 aagctgattg gcgcagctgc cagcacaaaa accagcgcca tcagcgtaga gaagccaagt
     6061 accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt gcggattatc atggtactgg
     6121 gcaacgtatt tggttacacc gttaaagatg ccagccccgg caagcacgcc gagcacggta
     6181 atcaactggc ggaaatttgc cgccagccca agccccgccg gaccaaatga caccgccagc
     6241 aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg tactggccgc cgtccacaag
     6301 gacgcttttg ccaacgacat atcaggagaa gtagttcaac aaagtcgcaa ttaccgtacg
     6361 ctgattgacg ggcgacaggt tgtagaacag cggcaggcgc agcaggcgct cgctctcttt
     6421 ggtggtgtag cgatcttcac cgtggaactc accaaagcgt tcccccgcag ggcaaccgtg
     6481 cagcggaatg taatggaaca ccgccatgat ttccgcttct ttcagaaagt taatcaacgc
     6541 gctccggtca ccaatatccc gcagtttaat gtaaaacata tgcgcgttct gcaagcagcc
     6601 atcgggaatc gacggcagct cgatacgccc ggctttcgcc agaggtgcta acgcatcgta
     6661 gtagttttgc cacagcgcca gacgttgctg gttgatacga tccgctgctt ccagttgcgc
     6721 ccacaggtac gcagcttgca gatcggacat caaatagctg gagccgatat cgcgccaggt
     6781 atatttatcg acctgaccac ggaagaactg gctgcggttt gtgccttttt cacggatgat
     6841 ctcggctcgt tcgatcaacg ctttatcgtt aatcagcgtc gcgccgcctt caccgcccgc
     6901 cgtgtagttt ttggtttcat ggaagctaaa gcagccaata tgaccaatgg ttcccagtgc
     6961 acgccctttg taagtggaca tcacgccctg agcagcatct tccaccacaa acagattatg
     7021 ctttttcgcc aacgccataa tggtgtccat ttcgcaggcc acgcccgcgt aatgcaccgg
     7081 cacgataacg cgcgttttgt cggtgatcgc cgcttcaatc aacgtttcgt cgatgttcat
     7141 ggtgtccggg cgaacatcca caaaaacgat ttttgcgcca cgcagcacaa aggcattggc
     7201 ggtggagaca aaggtgtagc tcggcatgat cacttcatcg ccaggctgga tatcgagcag
     7261 cagcgccgcc atctccagcg aagcggtgca ggacggcgtc agtaacactt tggcgctgcc
     7321 aaaacgttgc tccagccact gctggcagcg acgggtaaaa ccgccatcgc cacacagttt
     7381 gccgctaccc attgccgact gcatatagtc gagttcggtt cccaccaccg gcggtgcgtt
     7441 aaatggaatc atgtgatcac ctgtataacc agtacgcggt gctttctaca ttcgcaccac
     7501 tttgtatgta tcgtttaagc gcggcggtgt tgcccatttg ggtcgccacc cgcaaagttg
     7561 ttttaccgcg agcatacgcc cagtttagcg ccgtttgcat cagctcagca cctgcaccgc
     7621 gtccagccag caggccaatt cgcgcatctg tagcattgag ttcccgtaaa gagacataac
     7681 cgcgaatatc gccggacgcc gcacgtaaaa tcagacattg atgatcaaag gtgccgcgca
     7741 cggcattttc aatccactgt gcataaaagc gactgctggc gtcaggcgca taccacggcg
     7801 cacgaaaacg gctttgcgca aatgcggcgc tggctaactg acgtaatgcg ggaatatcgg
     7861 tctcttgtgc cactacagca ccgctatcac tggcattgtt cacgggtagc gccaaatcaa
     7921 cttcaccttc taccagggag aatcccagct gttgcagggc atccagttcc cccgtatttg
     7981 atgccgcaat tttggcctgc acccgtgacc acggcgctaa cgcgtctggc gtcaggaacg
     8041 gtgcttcaga cgtaatgcgc acgatggcgc tgttaacacc aaagaaggcg ttttcccagg
     8101 ttagtggctc aatactggcg cggacgggca cgaagtaact ccagcagata ttggccgtag
     8161 ccagttttcg ctaatgaact ggcagcacgc ttcacaccct cgtcatcgag ccagccgtta
     8221 cgccaggcga tctcttccag gcaggcaatc ttaaagccct ggcgtttttc caccgtctgt
     8281 acaaaggtgc tggcttcaat caggctgtcg tgagtgccag tgtccagcca ggcaaatccg
     8341 cgcccgagca gttcaacggt caggttgccc gcctcgaggt acatctggtt gatggaggta
     8401 atctccagtt caccacgctc cgacggcttc acctgctttg cgtactccac gactttactg
     8461 tcgtagaaat aaagcccggt caccgcccag tttgatttcg gctgttttgg cttttcttcc
     8521 agcgagatag cgcggaaatt atcgtcaaac tccaccacgc caaagcgttc cgggtccatc
     8581 acctgatagc caaaaaccgt cgccacttcc gtgcgcgccg caacatgacg cagcttcgga
     8641 ctgaagccct gaccgaagaa gatgttatcg cccagcacca gacaagaagg ttcaccatta
     8701 aggaaggttt caccgatgat aaaggcctgc gccagaccgt ccgggctggg ctgttcggca
     8761 tattccagct ggataccgaa ctcactacca tcgcccagca ggcgctggaa ataaccttta
     8821 tcttccggcg tagtgatgat gagaatttcg cggataccgg ccagcatcag caccgacagc
     8881 ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt gcttcgatac gccgcgcgta
     8941 atcggatgca atcgggtacc ggaaccgccc gccaggataa tacctttcat gtttgcctcc
     9001 gctgaaaatt agcctttcag acctaaacgc tcgccctgat agctgccgtc ctgcacctgc
     9061 ttcctccagc tttcattagc cagataccac tgcaccgttt tacgcattcc actttcaaag
     9121 gtttcctgcg gcagccagcc aagttcacgg gcaattttcg aagcatcaat ggcatagcgc
     9181 agatcatgcc ccggacggtc agcgacaaag gtgatcaagt cacgataatg caccacgcct
     9241 tgcggcttgt tcggagccag ttcttccagc agctcgcaaa tggtttccac aacatcgaga
     9301 ttcttacgct cgttgtgacc accaatatta taggtttcac cgactttccc ggtggtcgcc
     9361 acgcaataca gcgcgcgggc gtgatcttca acatacagcc agtcacggat ttgctgcccg
     9421 ttgccatata ccggtagcgg tttacccgcc agcgcgttga ggatcatcag cgggatcagt
     9481 ttttccggaa agtggtaagg accgtagtta ttcgagcagt tggtgataag cgttggcaga
     9541 ccataggttc gcagccaggc gcgcaccaga tggtcgctgc tggcttttga cgcggaataa
     9601 gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat catccgtcga gtgcaggtca
     9661 ccatatactt cgtcagtgga gatatgatga aaacggaacg ctgatttttt atcttccgtc
     9721 agcgcattcc agtaagcccg cgccgcttca agcaatgtat aagtcccgac aatgttggtt
     9781 tcaataaacg ctgccgggcc gtcaatagaa cggtcaacat ggctttcggc tgccagatgc
     9841 atgacacagt ctggctgatg ctcagtgaat acgcgcgcca gttctgcccg atcgcagata
     9901 tcaactttct caaaggcaaa gcgctcgctt tgcgcgaccg gtgccagcga catcaggttt
     9961 ccggcgtagg tcagcttatc gaccactacc accgcgtcgc tcgtttcgtt gatgatataa
    10021 cgcaccagcg ccgagccaat aaacccggca ccgcctgtta tcagaatctt tctcatcagc
    10081 gccagactcc tttagcatcg acgacatact gctgatggac attgtcgcca ttgataactt
    10141 tgaactgact atgatcgacc agcatcacca gcacgtctgc cgttgccagc gcctcgtcaa
    10201 gctgcgccag agtacaaagc ccggtcagtt ttttcggcaa ctggtggatg ttaggctcaa
    10261 caaccagagt ttcgccgcta tgccactggg cgatcagttc agcgatttcc atcgccgggc
    10321 tttcgcgcag gtcatcaata ttcggtttaa acgccagacc aaagcaggcg attttcagtt
    10381 cactggcgcg tttatcggta gccgccaggc aatcagccac cgccgctttc acctgatcga
    10441 taacccagaa cggtttgtga tcgttcactt cgcgcgcggt acggataagc cgcgcctgct
    10501 gggggttctg tgccacgata aaccacggat caacagcaat gcagtgaccg cccacgccag
    10561 ggccaggctg aagaatatta acgcgagggt gacgattcgc caggcgaatc agttcccaga
    10621 cgttaatccc ctgatcggca caaatcagcg acaattcatt agcaaaagcg atattcacat
    10681 cgcggaagct gttttcggtg agcttacaca tttccgccgt ccgcgagtta gtgacgacac
    10741 actcaccttc gaggaaaatt ttgtacagtt cgctggcgcg ggccgaacaa accggcgtca
    10801 taccaccaat cacgcgatcg tttttaatca gctcgaccat aacctggccc ggtaacacgc
    10861 gttccgggca gtaagcaatg ttgacgtccg cctgctcgcc cacctgctgt gggaaagtga
    10921 gatccggacg catctctgct aaccattctg ccatcttctc ggttgacccc accggcgagg
    10981 tggattcaag gatcaccagc gcgccttttt tcagcactgg cgcaatggag cgagcagccg
    11041 attcaacgta ggtcatatct ggctcatgat cgcctttaaa cggcgtgggt acagcaatca
    11101 gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa aaaaccgcct tctacggcag
    11161 tttttactac gctcgccaaa tcaggctcga cgatatggat ttcgccacga ttgatggtat
    11221 caaccgcgtg ttggttgata tcgacaccaa tcacctgttt ttgccgtgag gcaaacgctg
    11281 ctgctgtagg cagcccgatg taacccagtc cgataacaga aatggtcgca aaactcatag
    11341 tgatatccga ttatttttta acgcttccag aatgcgagag catgcctgac catcaccata
    11401 cgggttatgg gcgcggctca tagtttgata ttcgttttcg tcttttaaaa gacgcgtcac
    11461 ttcctcgaca attcgctgct tatccgtgcc taccagacgc accgtacccg ccgtcaccgc
    11521 ttccggacgc tcagtggtat cgcgcatcac cagcaccggt ttccccagcg aaggcgcttc
    11581 ttcctgaatg ccgcctgagt cggtcaaaat cagccaggcg tggttcatca gccagacaaa
    11641 cggtaaatac tcctgaggat cgatcagaat gacatttttc acatgcccca gaatgcgatt
    11701 gaccggctct ctgacgttcg ggttgagatg caccggatag acaatctgga tgtcctggtg
    11761 cgtggtggcg atgtctgcca gcgcgtggca gatttcttca aagccacgac cgaagctttc
    11821 acgcctgtga ccggtcacca gaatcatctt tttatcgggg tcgataaacg ggtaatttgc
    11881 cgcaagttct gaacgcagcg tgtcgctgct catcacctgg tcacgcaccc ataacagtgc
    11941 atcaatgact gtattaccgg taatgaagat tcggctatct gcaacgtttt cacgcagcaa
    12001 gttttgccgg gaagtttcgg ttggagagaa atgatacatc gccagatgcc cggtcaatgt
    12061 acggttagcc tcttccggcc acggcgaata gagatcgccc gtgcgtaaac cagcctcaac
    12121 gtgaccaaca ggaatacgct gataaaacgc cgccaggctg gttgccagcg tcgtcgttgt
    12181 gtcgccgtga accagcacga cgtctggttt gaactcggca agaataggtt ttagcccttc
    12241 cagaatccga caggttatct ctgtcaggcc ctgtcctggc tgcattatgt tgagatcgta
    12301 gtcaggtaca atggaaaaga gtttcagcac ctgatcgagc atctcccgat gctgcgcagt
    12361 gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac gcatgcacca acggagccat
    12421 cttgatggct tccgggcgcg taccaaatac agtcagtact ttcacatcga ttctcttcga
    12481 ataagcggcg agcgcctttg cgctcaccgc agcagtgttg ctatttcgag caacggcggg
    12541 ttaatgcgac accagccccg atcagccccc cgacaatgcc ccacataatc atcaggaagg
    12601 cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt acgcaaatag cgataggtct
    12661 gaaaacgcgg atccagggtt ggaccaacat tcagggtgtt taacatggcc cgattctgat
    12721 catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt ttccagtcga gcctggagca
    12781 ttggacgccc aagcaggaac atttctgaat caggtaattc ctcggcaggc acatctgtcg
    12841 cactgcgcga aatattatgc tgctcagcaa ttttcagcgc ctgctcaatg ctgttcatcc
    12901 ggcggtcgta gatggctttc gccacctctt cctgacgctt cacctgtgct ttcatctgga
    12961 tggtacgcgc cgcccatgcg cctttcagct catcattcag atggctggct gcacgctggc
    13021 tggcaaaagc aacatactga cgtaacaggt tattagcgtc aggcgcggtt tcggcaataa
    13081 gcttcacgct gtcattgacc gcgcgggtaa agtctccggg gataaactgg atgttgttaa
    13141 tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc caccatccgc tgcttgtaat
    13201 agtcggtttg cagccagaac tctctgcggg tatcccacga ggccagttgc ataacaaact
    13261 ctttataggc ttcgtccatg accgatggtt ggtcggcaga agccatgttt gaacggacat
    13321 ccaggttacg caaaaattgc tgctgcgagt aatatccccc cagcatattc accgttggac
    13381 gatcggtaat cgccgtcgag ctccactcct gacgagcaaa aaacgtatac gccagcgcga
    13441 ttaacgcaaa cgccagcccc atgccaataa tccatagctt cccagcccac aaggtacgaa
    13501 acaacccacg aatatccagt tcattttcag cgtcttcggc cggtttccca ggcattggtt
    13561 gtgtcatcac atcctcattt atttggttaa attggggctg ccaccacgat ttctacgcag
    13621 tctgcgtttt acgcgcttaa taaagcgagc aactttccag gcacgcttaa tgcaatatcc
    13681 atagaggagg aatgctagca aaaagagcac caacatgacc cactccggga caaaatgaga
    13741 atattctgcc agcacgccaa tggaagcgag cagtgctgcg gcaagggtaa tcagcacaaa
    13801 cgcctggcgg gaagtaaacc cggcacgcat gatcaaatgg tgaatatgct gacggtcagg
    13861 agagaatggg ctcatgcctt tacgcaggcg acggtacata atcgccacca tatccattag
    13921 cggaatggcg attatccaca aagcggtaac cgggctgatg ggatgggttt tgccttgggt
    13981 cgtttcgagc aggatccaga tcacggtaaa accaatcagc gtactgcccg catcacccat
    14041 aaagactttg tagcggcgac ccaggatacc aaggttaagc atgatatatg gcaggatggc
    14101 ggcgatcatc gcaaagcacc agattgcgag gctggtttgc ccgtcgaacc acaaaatcat
    14161 accgattgct gcaaacgaga cgcaggacaa cccgcccagc aagccatcaa tgccatcaac
    14221 catgttgaac gcattaatgg ccgcccagac ggcaaatagc gtcaggaagt aaccaaacgg
    14281 tccgagcacc atctcccagg agccaaagat ataacccagg ctactgagat aaaggttgcc
    14341 aaacaccatc ataacaatgc caacagcggc ctgtatggtg gcacggattt ttacgctgat
    14401 atcaaaacgg tcatccagcg cgccaatgaa aacaagcaca ccggcacaag cgagatagag
    14461 agatgcatgc ggaatatagt aatcgacaat tccgaacgtg aagcaaatcc ctgcgtaaac
    14521 cgaaataccc ccaacgagtg gtatcaaccc ctggtgacgt ttgcggaagt ttggtttatc
    14581 cactaaaccg actttttttg ccaccttacg ggcaaaaaac agaaacagtg tcgtgaataa
    14641 aaaaatactg atgagatcag tactcactgt cagtaaattc acaatgcatg ctctcagaga
    14701 aaattattag cagaagtata accacgaaga cctttattca gaagggaaag tccgttccga
    14761 acccaccagt cctgtgcaaa aattaaccat ttgatatgca aattgcataa catgccagca
    14821 aattccagga attttcctaa catcgccagc gcggcattaa gattacagat ataaaagcaa
    14881 aacgccacgt aaacacgtgg cgtttttggc ataagacaaa tttatgagcg tttcatcatt
    14941 tcgaagaaat cgtcattggt cttggtcatt gccagtttat taatgaggaa ttccattgca
    15001 tcgatttcgc ccatcgggtg aatgattttg cgcaggatcc acattttctg cagttcttcc
    15061 tgagtcgtga gcagctcttc tttacgggta ccggaacggt tgtagtcgat agccgggaag
    15121 acgcgttttt cagcgatctt acgagagagg tgcagttcca tgttgcctgt acctttaaac
    15181 tcttcgtaaa taacttcgtc cattttagaa ccggtatcga taagcgccgt cgcgataatg
    15241 gtcaggctgc cgccctcttc cacgttacgc gccgcaccga agaagcgttt cggacgatgc
    15301 agggcgttgg catccacacc accggtcaac actttacctg acgccggaac aacggtgttg
    15361 taagcgcgcg ccagacgagt gatggagtcg agcagaatga taacgtcttt cttgtgctca
    15421 accaggcgtt tcgccttctc gatcaccatt tccgcaacct gaacgtggcg agatgcgggt
    15481 tcgtcaaagg tagaagcaac aacttcacct tttaccagtc gctgcatctc ggttacttct
    15541 tccggacgtt cgtcgatcag cagaaccatc agcacacaat ccgggtggtt gtaagcaatg
    15601 ctctgagcaa tgttctgcag cagcatggtt ttaccggctt tcggcggtgc cacaatcaga
    15661 ccacgctgac cacgaccgat aggtgatgcc agatccagta cgcgagcggt taaatcttca
    15721 gtagaaccgt taccacgttc catacgcaga cgagagtttg cgtgcagcgg ggttaagttc
    15781 tcaaagagga ttttgttgcg ggcgttttca ggtttgtcga agttaacttc gttaactttc
    15841 agcagcgcaa aatagcgttc accttctttc ggcgggcgaa tcttaccaga gatggtatca
    15901 ccagtgcgga ggttgaaacg gcggatttgg ctaggggaaa cgtagatgtc atcaggaccg
    15961 gcgaggtagg agctgtctgc ggaacggagg aaaccaaatc catcctgcaa tatctccagt
    16021 acgccatcac caaagatatc ttcgccactc tttgcgtgct gcttcaggat ggcaaaaata
    16081 atgtcctgct tacgcatacg agccaggttt tccagcccca tattttcgcc gagagtgatc
    16141 agctcagaaa ccggcgtatt ctttaattcg gtaagattca tagtggtgtg agttcttaaa
    16201 cttggggtaa atctcgaact taatgttgtg aatggtatgg caggatcatc catgcctgtt
    16261 tacggtcatc gactcatgtc ttttcgctgc ctggtcacag gagagtacgc agaactgaaa
    16321 cgacaagacg gattgagtga cgagccagaa atctgttcac ttcgcattta agataaaacg
    16381 ggaagcgttg gaaattacaa gattcaaact taataaggta tgtttaatac gaagtcaaca
    16441 ctaagttagc atgactcacg ccgggcgtcc agtttttagc gacggggcac ccgaacatga
    16501 aattccctta cgccaggtta gcgtcgagga actctttcaa ctgaccttta gacagtgcgc
    16561 ccactttggt tgccgccact tcaccgtttt tgaacagcag cagagtcggg ataccacgga
    16621 tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca acggtcagtt
    16681 tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg cacggaccgc
    16741 accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca tccgtgtcaa
    16801 aactgtcgtc agtcaggtga ataattttat cgctcatata taactccaca ggaataagcc
    16861 tggcgtgttg gtttcgttgt tggtgtaaca ttaaccaact aaaggttgac tttatttcac
    16921 cggatacgct ttcgtaaagc aatagtaagc tgatattcta ccacactatg agcaaaacac
    16981 atttaacaga acagaagttt tccgacttcg ccctgcatcc gaaggttgta gaagcccttg
    17041 aaaaaaaagg gtttcataac tgtacgccca ttcaggcact ggcccttccg ctgacgctgg
    17101 cgggtcgtga cgtagccggg caggcgcaaa ccggtaccgg gaaaacgatg gcgtttctta
    17161 cgtcaacgtt tcattgtctt ctctctcatc ccgcgattgc cgatcgcaag gtgaatcagc
    17221 cgcgtgcctt aattatggca ccgacgcgtg aacttgccgt gcagatccat gccgatgcgg
    17281 aaccgctggc ggaagctacc ggcctgaagc tgggtctggc ttacggtggt gatggttacg
    17341 acaaacagct gaaagtgctg gaaagcggcg ttgacattct gattggcacc acggggcgtt
    17401 taattgacta cgccaagcag aaccacatta acctcggtgc cattcaggta gtggtactgg
    17461 acgaagccga tcgcatgtac gatctgggct ttattaaaga tatccgctgg ctgttccgcc
    17521 gaatgccgcc tgcaaaccag cgcctcaaca tgctgttctc cgccacgctt tcgtaccggg
    17581 tacgtgaact ggcgttcgag cagatgaaca atgccgaata tattgaagtg gaaccggaac
    17641 agaaaacggg ccaccgtatt aaagaagagc ttttctaccc ttctaacgaa gaaaaaatgc
    17701 gtttgctgca aacgctgatc gaagaagagt ggccagacag agcgattatt ttcgccaata
    17761 ccaaacaccg ttgtgaagag atctggggcc acctggcggc agatggtcat cgtgtcggtt
    17821 tgttgacagg cgatgtcgcg cagaaaaaac gtctgcgtat tcttgatgaa tttacccgtg
    17881 gcgatctgga tattctggtt gccaccgacg ttgccgcgcg tggtttgcat attccggcag
    17941 tgacgcacgt ctttaactac gatttacccg atgactgtga agattacgtt caccgtattg
    18001 gtcgtacagg tcgcgcaggc gcaagcggtc actctattag cctggcgtgt gaagagtatg
    18061 cattgaattt gcctgctatt gagacctata ttggtcactc aattccggta agcaaataca
    18121 atccggacgc attgatgacc gatctgccaa aaccgctgcg cctcacgcgc ccgcgcacag
    18181 gcaatggtcc gcgtcgtact ggcgctccgc gtaatcgtcg tcgttcaggt taaataaaaa
    18241 atgtcatact caaaatcatt caaactgtat caagatggca agagaatgaa tcccgatgag
    18301 cttactgtag taagtgattc aggtgaaaga atgcagccaa cacagagaca gattgaagga
    18361 tgaagagtat gggttccacc tcatcgctgt atgcagccat tgatctcggt tcgaatagtt
    18421 ttcatatgct ggttgtgcgc gaggtggctg gaagcatcca gacgctgacg cgaataaaac
    18481 gcaaagtgcg tctggctgct ggcctgaata gcgaaaatgc cctctccaat gaagcaatgg
    18541 agcgcggttg gcaatgtttg cgcctgtttg ctgaacgtct gcaagatatt cctccctcgc
    18601 aaattcgcgt tgtcgctacg gcgacgttac gccttgccgt caatgcgggt gattttattg
    18661 ccaaagcgca ggaaatcctc ggttgtccgg tacaggtgat cagcggtgaa gaggaagcac
    18721 gtctgattta tcagggtgtt gctcacacca ctggcggtgc cgatcagcgc ctggtagtgg
    18781 atatcggcgg tgccagtact gaactggtaa ccggcacggg tgcacaaacc acctcgttgt
    18841 tcagcctgtc gatgggctgc gtcacctggc tggaacgcta ttttgccgat cgtaatctgg
    18901 ggcaggaaaa ttttgatgct gcagaaaaag cggcacgcga agtgttacgt ccggttgccg
    18961 atgaattacg gtatcacggc tggaaagtgt gcgttggtgc ttccggcacc gtgcaggcgt
    19021 tacaggaaat catgatggca caggggatgg atgaacgcat taccctggaa aagttgcagc
    19081 aattgaaaca gcgagccatt cattgcggtc ggctggagga actggagatt gacgggctga
    19141 cgctggaacg tgcgttagtg ttcccgagtg gtctggcgat cctgatcgcc atttttaccg
    19201 aactgaatat tcagtgtatg accctggcgg gcggtgcgct gcgtgaaggc ctggtctacg
    19261 gcatgttgca tctggccgtc gagcaggata ttcgcagccg tacgctgcgt aatattcaac
    19321 gccgctttat gatcgatatt gatcaggcac agcgcgtagc caaagttgcg gctaacttct
    19381 tcgatcaggt ggaaaacgaa tggcatcttg aagcaataag ccgcgatttg ctcatcagcg
    19441 cctgtcagct tcatgaaatc ggcctgagcg ttgacttcaa acaagcgccg caacacgctg
    19501 cttatctggt gcgtaatctg gatcttcccg gttttacccc cgcacagaaa aaattgctgg
    19561 cgacgctact gctcaaccag actaatccgg tcgatctctc atcgctgcat cagcaaaatg
    19621 ccgtaccgcc gcgcgtcgca gaacaactct gccgtttact acgcctggcc atcatttttg
    19681 ccagccgtcg ccgtgacgat ctcgtgccag agatgacatt acaggctaac catgaactgt
    19741 tgaccttgac gcttccgcaa ggttggctaa cccaacatcc gctgggtaaa gagattattg
    19801 ctcaggaaag ccagtggcag agctatgtcc actggccgct ggaagtgcat taatgagtaa
    19861 gtgccggatg cgatgctgac gcatcttttc cggccttgat tatttccctc gttttgccgc
    19921 catcatcgct ttcagattcg ccagatggct ttgccctttc tgcatccgtt cttcggcgct
    19981 gaccactttg cgctcctgtt cccaaatcag atcatcctgc ggcagctcca gcaaaaagcg
    20041 gctcggctcc gggcgcacca gttcgccgta ctgacggcgt tctttacaca gcgtaaaggt
    20101 caattccttc tgggcgcggg taatgccgac ataggccagc cgccgctcct catcgatatt
    20161 atcttcatcg atactgctct ggtgcggcaa aaacccttct tccataccga ccatgtagac
    20221 ataaggaaac tccagtcctt ttgacgcgtg gagagtcatc agttgcacct gatccagctc
    20281 ttcttcactc tcaccacgct ccatcatgtc gcgcaaagta aagcgcgtca ccacctgggt
    20341 gagcgtcatc ggctcatcca gttcgctgcc ttccagcatc tccgtcatcc agctaaacag
    20401 ttggttgacg ttcttcatgc gcatttcggc ggctttcggg ctgggcgatg tttcgtacag
    20461 ccaggattca taatccatgc catggatcag atcacgcacc gcagcaatcg gctcccgctc
    20521 cgccaggcgc tggatttctg ccaaccagtg agtgaagcgg gtcaatgctt cataaccacg
    20581 tccgctaagc gtctgactca ggcccatatc aaagctggcg gtaaacatgc ttttattgcg
    20641 cgtcatcgcc cactcaccca gctttttcag cgtagccggg ccaatctctc gcttcggcgt
    20701 gttaacgata cgcagaaatg cgctgtcatc gtccgggtta gtcagcacgc gcagataagc
    20761 cagcaagtcc ttgatttcag gacgagagaa aaacgacgta ccaccggata ttttgtacgg
    20821 gatgcggttt tgcatcagga atttttcaaa tacccgcgac tgatggttgc cgcgataaag
    20881 aatggcgtaa tctttgtact gcgttttatt gacgaagtga tgggcgatca gctcgccagt
    20941 aacgcgctcg gcttcatgtt cctcgttatt cgcgcttaaa acttttagct ctgtgccgta
    21001 acccagttcg gagaacagac gcttttcaaa gacgtgcggg ttattggcga tcaggatgtt
    21061 cgccgctttc agaatacgcc cggaagagcg atagttctgc tcaagcttaa tcaccttcag
    21121 cgccggaaaa tcctgactca gcagcaccag gttttgcgga cgtgcaccgc gccaggagta
    21181 gatcgactgg tcatcgtcac ccaccacggt aaagcgcgcg cggctgccca ccagcagttt
    21241 caccagttca tactggctgg tgttggtgtc ctgatactca tccaccagca ggtagcgaat
    21301 tttgttctgc cagcgttcgc gtacttcttc attgcgttgc agcagcaacg tcggcaataa
    21361 aatcaggtca tcgaagtcga gaacgttaca ggctttcagg tgtgcatcat acagcccata
    21421 acaatgggca aaaatacgat cccgctcgcc ctttgcctct gccgccgcct gcgccggagt
    21481 tttgagatcg ttcttccagt tagagatggt cgaaatcagt tgttgcagga gaactttgtc
    21541 atcttcaatc agcccctcgg tcaactcttt aagcaaagca agctgatcgg tatcgtcaaa
    21601 taacgagaag ttcgctttca tcccaagcgc cgcatactcg cgtttgatga tatccagccc
    21661 caacgtatgg aaagtggaga tcatcagccc acgcgcctct ttacgcccca gcgtctgccc
    21721 tacacgctct ttcatctcgc gcgctgcttt attagtaaag gtcaccgccg caatgtgccg
    21781 cgcctgataa ccgcaaccgc ggatcagatg ggcgatttta ttggtgataa cacgagtttt
    21841 accggaaccc gcgcccgcca gcaccaggca ggggccggta acgaattcga cagcttgttg
    21901 ttggccgggg tttagacgca taggtgtatt gctcaatctt cgaacggggg gaggattgta
    21961 gcagaatcgc atttgaatgt gaccgcgtgt aagccgctaa ccggttggct gtggatgttt
    22021 aaatcagaga gagaagaccg aaatcggagt tcatcgtgaa tccattagac ttagaaaata
    22081 tcgggtcgta acgttgtgtt attacttttt cagagtttac taaggagagg tttactcgtg
    22141 gcgattaatt tcagccctaa ggttggtgaa atactggaat gcaactttgg taactatcct
    22201 gttagccaaa atggaccgtt cagcacgacg tattacgatg gccgcattcc tcctgaaatg
    22261 ataaaaaaca ggttagttgt cgtgctcaac ggaaaaatta atggcaacgc attcatcgtc
    22321 gttccattat caaccactcg cgaccatgac aagttaaaac gtggaatgca tgttgaaata
    22381 gccagtaatg ttatcaatga cctgcaattc tttgatcagc agattcgttg ggccaaaacc
    22441 gacctggtac aacaagtcag tcgaaacaga ttaaacagag caagaactta cagagggtat
    22501 cttaatcaat gtttgccaca tgagttggtt gcagacatac agagggcggt catcaagtca
    22561 atcaatgcca tatcgctcat aaattgacaa taaaatcacc atccccgata atgactttgt
    22621 agcccataga ggccctaagt tttctcccct tgagggacct gcgaagcccg gattttccgg
    22681 gcttttgcca tatatgtacc agcccctccc tgactgacaa ttacgttagc atcggcggat
    22741 catgctaaaa tcgccgcctg acaattattc actcaaggca cgatcatggc aaaaacagca
    22801 gcagcactgc atatccttgt aaaagaagag aaactggctc tggatcttct cgagcagatt
    22861 aagaacgggg ccgattttgg caagctggcg aagaaacact ccatttgccc atcaggcaaa
    22921 cgcggcggtg atttaggtga attccgccag ggtcagatgg ttccggcgtt cgataaagtt
    22981 gtgttctctt gtccggtact ggagccaacc ggcccgctgc acacccagtt cggttaccac
    23041 atcattaagg tgctgtaccg taactaatag caaggccttc tccgggagaa ggccttgaac
    23101 tctgaatcgc tattaacccg caacagcaat acgtttcata tcagtcatgt agccgcgcag
    23161 tttcttacct acctgctcaa tcgcatggct gcgaatcgct tcgttcaaat cacgcagttg
    23221 cgcgttatct accgcacctt ccggaatagc tttacccaaa tcgcccggtt gcagctctgc
    23281 cataaacggt ttcagcaacg gcacacaagc gtaagagaac agatagttac cgtactcagc
    23341 ggtatcagag ataaccacgt tcatttcgta cagacgctta cgggcgatgg tgttggcaat
    23401 cagcggcagt tcgtgcagtg attcatagta agcagattct tcgatgatgc cggaatcaac
    23461 catggtttca aacgccagct caacgcccgc tttcaccatc gcgatcatca gtacgccttt
    23521 atcgaagtac tcctgctcgc cgattttgcc ttcatactgc ggcgcggttt caaacgcggt
    23581 tttgccggtc tcttcacgcc aggtcagcag tttcttatca tcgttggccc agtccgccat
    23641 catgccggag gagaattcgc cggagatgat gtcgtccata tgtttctgga acagcggtgc
    23701 catgatctct ttcagctgtt ctgacaacgc gtaagcgcgc agtttcgccg ggttagagag
    23761 acggtccatc atcagggtga tgccgccctg tttcagcgct tcggtgatgg tttcccagcc
    23821 gaactgaatc agtttttctg cgtatgccgg atcggtgcct tcttccacca gcttgtcgaa
    23881 gcacagcaga gagccggcct gcaacatacc gcacagaatg gtttgctcgc ccatcaggtc
    23941 agatttcact tccgcaacga aagaggattc cagcacgccc gcacgatgac caccggttgc
    24001 cgccgcccat gctttagcga tcgccatgcc ttcgcctttc ggatcgtttt ccgggtgaac
    24061 ggcaatcagc gtcggtacgc cgaatccacg tttgtactct tcacgtactt cggtgcctgg
    24121 gcatttcggc gcaaccatta ctacggtgat gtctttacgg atctgctcgc ccacttcgac
    24181 gatgttgaaa ccatgcgagt aacccagcgc cgcgccgtct ttcatcagtg gctgtacggt
    24241 gtgcactaca tcagagtgct gcttgtccgg cgtcaggtta accaccagat ccgcctgtgg
    24301 gatcagttct tcgtaagtac ccactttaaa accattttcg gtcgctttac gccaggacgc
    24361 gcgcttctcg gcaatcgctt ctttacgcag agcgtaggag atatcgagac cagaatcacg
    24421 catgttcagg ccctggttca gaccctgtgc gccacagccg acgatgacca cttttttacc
    24481 ctgaaggtag ctcgcgccat cggcgaattc gtcgcggccc ataaagcgac atttgcccaa
    24541 ctgtgccagt tgctggcgca ggttcagtgt attgaagtaa ttagccatgg tgattcctcg
    24601 tgatgttgtg cttcttattg ttcggttcgc gtttgcaaga ttgaattcac tatatgacag
    24661 gaaatttatt gcggaaattg atatattcac aacatcatat tgcaattttt gcaacgtcaa
    24721 catcgagggc tgtccctgtg gatttacgcg atctgaaaac cttcctgcat ctggcggaaa
    24781 gccgccattt tggccgcagc gcgcgggcga tgcacgttag cccatccacg ctctcacggc
    24841 agattcagcg cctggaagaa gatctcggtc agccgctgtt tgtgcgcgat aaccgcacgg
    24901 tgacgctgac tgaagcgggc gaagaactgc gcgttttcgc ccagcaaacg ctgttgcagt
    24961 atcagcagct gcgccacacc atcgatcagc aagggccgtc gctctctggc gaattacata
    25021 tcttctgctc ggtgaccgct gcctacagcc atctgccgcc gattctggat cgcttccgcg
    25081 cggaacaccc gtcggtggag attaaactca ctactggtga tgcggcagat gcaatggaaa
    25141 aagtggtcac gggagaagcg gatctggcga ttgccgggaa accggagacg ctacccggcg
    25201 ctgtagcgtt ttcgatgctg gagaatctgg cggtagtgct gattgcgccc gcgctgccct
    25261 gcccggtgcg taatcaggtc tcggtagaga agccggactg gtcaacggta ccgtttatca
    25321 tggccgatca ggggccagta cgccgccgca ttgaactgtg gtttcgacgc aataaaatca
    25381 gcaacccaat gatttacgcc acggttggcg ggcatgaagc gatggtatcg atggtggcgc
    25441 tcggctgtgg tgtggcgctg ttgccggaag tggtgctgga aaacagcccg gagcctgtac
    25501 gcaaccgggt aatgatttta gatcgcagcg atgagaaaac gccgtttgaa ctgggcgtgt
    25561 gtgcacaaaa aaagcggctg catgagccgc tgattgaggc gttctggagg attttgccga
    25621 accacaaatg acgttgtcgc gcgagtaggc ctgataagcg aagcgctatc aggcattttt
    25681 ccctaacccg ccaaaaagaa cctgaacgcc gggttattgg tttcgtcgtg gcaatcgtag
    25741 cccagctcat tcagccgggt ttcgaaatcc ggttcatggt cgccaagttc gaacgccgcc
    25801 agtacgcgcc cgtagtcggt gccatggctg cgatagtgga acaaagaaat gttccagtgc
    25861 gtacccagcg tgttgaggaa gcgcagcagc gcgcccggtg attccgggaa ttcgaagctg
    25921 tagaggcgtt cctgcaacgg atgtgacgga cgcccgccga ccatatagcg cacatgcaac
    25981 ttcgccattt cgtcgtcgga gagatccacc acgctgtagc cgccgtcgtt gagcatctgc
    26041 aaaatttctt tgcgctcttc gaggccgcgg ctcaggcgca cgccgacaaa gatgcaggcg
    26101 tttttggcat cggcaaaacg gtagttgaac tcggtgaccg atcgcccgcc aagcagttgg
    26161 cagaatttga ggaagctgcc tttttcttcc ggaatggtca ccgccaacaa cgcttcacgc
    26221 tgttcgccca gttcgcagcg ttccgagacg tagcgcaggc cgtggaagtt cacgttggca
    26281 ccggaaagaa tatgcgccag ccgttcgccg cgaatgttgt gctgggcgat atattttttc
    26341 attcccgcca gcgccagcgc gccagagggt tccgccaccg cgcgcacatc ttcgaataaa
    26401 tccttcatcg ccgcacagat cgcatcacta tcgacggtga tgatatcgtc gagatactcc
    26461 tggcacaaac ggaaggtttc gtcgccgatg cgttttaccg caacgccttc agcaaacagc
    26521 cccacgcgcg gcagatcaac cggatgaccc gcatccagcg ctgctttcag gcaggcggag
    26581 tcttccgctt ctacggcgat tactttgatt tgcggcatca gttgtttgat cagcaccgcc
    26641 acgccagcag ccagaccgcc gccgccgact ggcacaaata cgcggtcgag atgggcgtcc
    26701 tgctggagca gttccagcgc cagcgtgcct tgcccggcaa tcaccatcgg atggtcgaac
    26761 ggcggcaccc aggtgaaccc ctgctgctgt gacagttcga tcgctttggc tttcgcttca
    26821 tcaaagttcg cgccgtggag cagcacttcg ccgccgaagc cgcgcaccgc gtcgactttg
    26881 atgtcggcgg tggcggttgg cataacgatc agggccttca cgcctaaccg cgcagaagaa
    26941 aacgcgacgc cctgcgcgtg gttacccgca gaagcagtga tcacgccgtg cgctttttgt
    27001 tcttccgtca ggcccgccat catggcgtat gcgccgcgca gcttaaagct gtgcactggc
    27061 tggcgatctt cgcgcttcac cagaatcacg ttatcaagac gagacgagag cttttccatt
    27121 ttttgtagcg gcgtgacctg cgccgcttcg tagaccggcg cgcgcagcac cgctcttaaa
    27181 tattcagcac cttccggggc accggacagg ggttgcgagt cagccattat taacccccca
    27241 gtttcgattt atcgcgcacc gcgcctttgt cggcgctggt tgccaggctg gcataagcac
    27301 gcagggcaaa ggagatctga cgttcacgat ttttcggcgt ccaagctttg tcaccccgag
    27361 cttcctgcgc ttcacgacgc gccgccagtt cggcatcgct tacctgtaac tgaatgccac
    27421 ggttcggaat gtcgatggcg atcaggtcgc cgtcttcaat caggccaatg ctgccgccgc
    27481 ttgccgcttc cggtgagacg tggccgatag aaaggccaga ggtgccgcca gagaaacgac
    27541 cgtcggtgat cagcgcacag gctttaccga gccccattga tttcaggaag ctggttgggt
    27601 agagcatttc ctgcatcccc ggaccacctt ttgggccttc atagcgaatt actaccacat
    27661 ctccggcgac aactttaccg ccgagaatcg cttctaccgc gtcgtcctgg ctttcgtaca
    27721 ctttcgccgg gccggtgaat ttgaggatgc tgtcatcgac gcccgcagtt ttaacgatgc
    27781 agccgttttc cgcgaaatta ccgtagagca ccgccaggcc gccgtctttg ctataggcgt
    27841 gttccagcga gcggatacag ccattggcgc gatcgtcgtc cagcgtatcc caacggcaat
    27901 cttgcgagaa tgcctgggtg gtacggatac ccgcagggcc ggcgcggaac atatttttta
    27961 ccgcgtcatc ctgggtcagc ataacgtcgt attgttccag cgtttgcggc aacgtcaggc
    28021 caagtacgtt tttcacatca cggttcagta accccgcacg atccagctcg ccgagaatac
    28081 cgataacacc accagcacgg tgaacatctt ccatatggta tttttgggtg ctcggcgcga
    28141 ctttgcataa ctgcggaacc ttgcgggaaa gcttatcgat atcactcatg gtgaagtcga
    28201 tttccgcttc ctgcgccgcc gccagcaggt gaagtacggt gttagtcgat ccacccatcg
    28261 cgatatccag cgtcatggca ttttcaaacg ccgctttgct agcaatattg cggggcagtg
    28321 cactttcgtc gttttgctcg tagtaacgtt tggtcaattc aacaatgcgt ttaccagcat
    28381 tgaggaacag ctgcttacgg tcagagtggg ttgccagcag cgagccgttg cccggctgcg
    28441 acagacccag cgcttcggtc aggcagttca ttgagttagc ggtaaacatc ccggagcagg
    28501 aaccgcaggt tgggcatgcg gaacgttcaa cctgatcgct ctgggagtca gatactttcg
    28561 ggtctgcgcc ctggatcatc gcatcaacca gatcgagctt gatgatctga tcggacagtt
    28621 tggttttccc ggcctccatc gggccgccgg aaacaaagat caccggaata ttcagacgca
    28681 gggaagccat cagcatcccc ggggtgattt tgtcacagtt agagatgcag accatggcgt
    28741 cggcgcagtg ggcgttgacc atatactcaa cggaatcagc gatcagttcg cgagatggca
    28801 gtgaataaag catccccccg tggcccatgg caatcccatc atccaccgca atggtgttga
    28861 actctttggc aacgccgcca gccgcttcaa tttgttcggc gaccagttta ccgagatcgc
    28921 gcagatggac gtgacccggt acaaattggg tgaacgagtt cacaaccgcg ataatcggct
    28981 taccgaaatc ggcgtcggtc attccggtgg cgcgccacag cgcacgagca cccgccatat
    29041 tacgaccatg agtggtggtg gcggaacggt acttaggcat actttattta ctcccagtgt
    29101 ctgtctcgta aatgggacgg tgcgtgccgt cccatttttt gtatttattg attaacttga
    29161 tctaaccagc cccatttatc ttcggtttcg ccggtaaaga ggccgaagaa ggcttgctga
    29221 atgcgtttgg tgaccgggcc acaacggcct tcgccaacct gaataccgtc tacactgcgc
    29281 actggcgtga tttctgccgc cgtaccggac ataaagactt catccgccag gtacaaggat
    29341 tcgcgcgaca gcacctgctc acgcacttca attcccagct ctttcgccag tttgatgatg
    29401 gcatcacggg taatacccgg cagcgcggag gaggtgaacg gtggggtgaa cagcacacca
    29461 tctttcactt caaacaggtt ttcgcctgcg ccttcagaga tataaccgtt cacatccagc
    29521 gcgatacctt cctgataacc gtggcggcgc gcttcgctac ccaccagcag ggaagagaga
    29581 tagttaccac cggcttttgc cgcagttggg atggtgtttg gtgctgcgcg gttccaggag
    29641 gaaaccatcg catcgatccc ctgctccagc gcttctgcgc ccagatacgc tccccacggg
    29701 aaagcggcga taatcacatc ggttgagtat cccgctggcg ggtttacgcc catgccaaca
    29761 tcaccaacga agatcagcgg acggatatag gcgctggtga gattgttttt gcggatcacg
    29821 tcacggcaag cttccatcag ctcatcaatg ctctgcgaaa ccgggaagcg atagattttg
    29881 gcggagtcat gcagacgctg catatgctca cgatggcgga atacaaccgg tcctttgtgc
    29941 gagtcgtagc aacggatgcc ttcaaaaacc gaggtgccat agtgcagcgc gtgcgacatc
    30001 acatgcacct tcgcgtcttc ccagcgaacc atctccccat tgaaccaaat gtaatcagct
    30061 ttcttcgtgg tcatttttat attccttttg cgctcaggcg cggatttgtt gtgatgtggt
    30121 tgtgctctgg cagatggcaa cgtgtgcgac gtccaccagt ttatttaact gactaaacag
    30181 taagtcgacc gaccgtgggc tggcaacggt caattcgata tttatatttt gtgcatcgct
    30241 ggcggcggcc atattcattg agcagacgtg gaaaccacga tgacgcacca cgcgtaaaac
    30301 acgttctaag gtttccggat tgaagcgagc cgatacattg acctgatgtt gcatcatgat
    30361 aatttctcca acatttctga attactggca ccaggcggca ccagcggcca gacgttctca
    30421 agttcgtcga ttgagacatg aagcaggtat ggcccatcac tgttcagcat ggtgtcgagt
    30481 gccgcttcaa cctggtcttt acgggtgatg tgttggccag ggatgccgaa ggcgctggct
    30541 aacttgagga aatcggggtt atcggtaagg gtggtttcgc tgtatcgttc ctggaaaaac
    30601 agttgctgcc attgtcgaac catccctaac cgttggttat cgagtaagac gattttcaac
    30661 ggtaactgct tgcgttttac ggtgcccagc tcttgtacat tcatcatgaa agagccgtca
    30721 ccggagatac agacgacggt atcgtttggt cgcgcgactt gcgcgccaac cgccgccggt
    30781 aaaccaaaac ccatggtgcc taagccgctg gaggtaatga aattttccgg gcgagtgtgt
    30841 gcgatgtgct gcgcggccca catctgatgc tgccccacat ctgtggtcac aacgcaatcc
    30901 gcaggtttac gatccgacag ttgttttaac aacaacggcg cgtagatagc gtcaccggga
    30961 tggtcgtaac gccaggcatg ttcatcacgc agctgcgcgc agtattgctg ccagtcattg
    31021 atatttaatg gctgctgtaa tgctggtaac agcgcattta aatcaccctg taatgccaca
    31081 tgtgcctgac gcagcttgtt catttctgcc gggtcgatat ccatatggat aacactggcg
    31141 tgtggtgcga aggtgttcaa tttgccggtc acccggtcat caaaacgtgc acccacggcg
    31201 atcagcaagt cgcactcctg caccgcgaag ttcgccgctt tggtgccatg cattcccagc
    31261 atgcccagat agtacggata atctgcttca actgcgccca gccctttcag cgtgcaggtg
    31321 gcaggcattt ttgtggtagc gagaaattct cgtaaagcag gaactgcctg cgccataccc
    31381 acgccaccac caacgtacag catcggtttt tgcgcttttg ccagcatctg gcgcgcttgc
    31441 tcaacttcgg catgtgggaa agtcacttcg ttttcaacgg tggtgaacca cggttccagg
    31501 tcgccgctgg ctaactggat atcttttggg atatcgacca gaaccggacc aggacgacct
    31561 gagctggcaa cgtcgaatgc ttcagccata atgcgcggca actcttccag cgactgcacc
    31621 agaaagctgt gcttggtaca ggctaacgac aatcccagga catccacttc ctgaaatgcg
    31681 tcagtaccga taaacggtgc ggacacttga ccggtgatgg caacaacagg gatagaatct
    31741 aacagtgcgt ccgcaagccc ggttatcagg ttggttgcgc ccggaccaga cgtggcgata
    31801 catacgccag ttttgccggt agcacgggca taaccgatag ccgccattgc cgcaccctgc
    31861 tcatgtcggc acagcaagtg ctccacgccg ccgtcataca atgcatcgta aaccggcata
    31921 attgcgccac ccggataacc gaaaacggtg ttcacaccct gtgcccgcaa cgcatgtacc
    31981 acccactgtg cgccattcat agttagttcc ccgtcctgaa tcttgggaaa cagaattttg
    32041 tgctgttatt cattgtctgc tcctcggtta tgtttttaag gtcaaaaaaa acccccggac
    32101 ctttcggtgc gggggtctta gttcgttaag gcttgatctc taagcctttc ctcgtccaag
    32161 tgcagccccg cacggtggga taataatcac caccacgcta atcacgacca ggctaatcac
    32221 tcgtagaagg gctgtcattt tgtcttttct tgcatcttgt tcgaaggaat gcctaaagag
    32281 ttaccataga ttttgcgaat agcacaagat attttttggc cgttttttat gatggcttta
    32341 ataatttcaa tagaaattgt tgttttttaa aaagaaaata gtgagtgaaa aaaattcaat
    32401 ttcagtacga atgttgttcc ctccctcgta gttcatcctt tgtccgctgc atttttgcga
    32461 gcatcattcc accgccagaa caactcaact tcacttgcac aaataaacag gaatcagcca
    32521 ctaaaattca cgaattagcg ggctacagca gcgatatctt atgcacataa tggtgactca
    32581 aggagggctt atgtcactgt caattgttca tacccgcgca gccctgggag taaatgcgcc
    32641 cccaatcact gttgaggtac atatcagtaa aggtttgccc ggcttaacga tggtgggctt
    32701 accagaaaca acggtaaaag aagctcgcga tcgcgtgcgc agcgccatta tcaatagcgg
    32761 atatgaatat ccggcgaaaa aaatcaccat taaccttgct ccagccgatc taccaaaaga
    32821 agggggacga tatgatttac ctatcgccat tgcgttgctg gcggcctcag aacagcttac
    32881 agccaataag ttagatgaat atgaattagt cggagaactg gcgcttacag gcgctctgcg
    32941 tggcgttccc ggtgcaatct ccagtgcaac tgaagctatt aagtcgggca gaaaaattat
    33001 cgtcgcgaaa gataacgaag atgaagtggg gctaattaac ggtgaaggat gcctggtagc
    33061 cgatcatctg caagctgtct gtgcgtttct ggaaggtaag cacgctctcg aacgcccgaa
    33121 accaactgat gcagtatccc gggcgctaca acatgatctc agtgatgttg tcggtcagga
    33181 gcaaggaaag cgaggactgg aaattaccgc cgctggcggg cacaaccttt tactgattgg
    33241 gccgccggga acaggtaaaa caatgctcgc cagccgtatt aatggtcttt tgccagattt
    33301 aagcaatgaa gaggcactgg agagcgctgc gatattaagt ctggtaaatg ctgaatcagt
    33361 acaaaaacaa tggcggcagc gcccgttccg ctcacctcat cacagtgcgt cgttaacggc
    33421 gatggtgggc ggtggtgcaa ttccagggcc aggtgaaatt tcgttggcgc ataacggcgt
    33481 gctttttctt gatgagctac ctgaatttga acggcgtaca ctggatgctt tgcgagagcc
    33541 gattgaatcc gggcagatcc atctttcacg cacacgagca aaaataacct atccagcccg
    33601 tttccagctt gttgcggcga tgaatcccag ccctaccgga cattatcagg gaaaccataa
    33661 ccgctgcacg ccagaacaga cattacgtta tctcaaccgg ctctcggggc cctttctcga
    33721 ccgcttcgat ctctcactgg agatcccatt accacccccc ggcattttga gtaaaacggt
    33781 agtgccggga gaaagcagca ccaccgttaa acaacgtgta atggctgcca gagagcgcca
    33841 atttaagcgg cagaataagt tgaacgcctg gctggatagt ccggaaatac gcaaattctg
    33901 caagcttgag agcgaagatg cgcagtggct ggaagaaacg ctgatccatc tggggttatc
    33961 gattcgtgcc tggcagcggt tattgaaagt tgcacgaacc attgctgata ttgatcagtc
    34021 tgacattatc acacgtcagc atttgcagga ggcagttagc tatcgtgcga ttgaccgttt
    34081 gctcatccat ctgcaaaaac tactgacata aaaaaagggc atttcgccct ttttattaat
    34141 cgtcagaatc ggtgtagtct tcagcacctt caacctgcgg tttaccgccg gaaagggtgt
    34201 gaaaacgttt tggacgcttg atacgcgtca tatacttgga ccacacgcgt tctgcttctg
    34261 tcactggctc acgttcgcca cggcatactg ctacgaagag tttctcttcc tcggtaaccg
    34321 gctcgcgttt gccaagatcc aactcattga aggcataacc atgacgctca agcagttgtg
    34381 cctctttgat ggtgaaatca ccatggcgag agaatccacg tggataatgt ttattgtcga
    34441 aatatcgatt agtcgtcgta aagctttccg ccatcctgca cgctcctaat tctttgaccg
    34501 agctagttat ggcgcggagt attagttacg cttgacagag tgtaaaacaa aacatttaaa
    34561 tcataacgac aaataatttt gcggagagca ctgtggatac ggaattgtta aaaactttcc
    34621 tggaagttag ccgaacgcgt cactttggtc gagcggctga atcgctctat ctgacccagt
    34681 cagcagtgag ctttcgaatc agacaactgg aaaatcaact gggtgtgaac cttttcaccc
    34741 gccacagaaa caatatccgt ttaaccgctg ccggtgaaaa actactgcct tatgcagaaa
    34801 cgctcatgag cacctggcag gccgcccgta aggaggtggc gcatacctca cgacataacg
    34861 agttttctat cggtgccagc gcctcattgt gggaatgtat gcttaatcag tggctgggac
    34921 gtttgtatca aaatcaggat gcccatacag gcttacagtt cgaagcgcga attgcccaac
    34981 ggcagtctct ggtaaaacag ctgcatgaac gccagcttga tcttcttatt accactgaag
    35041 cgcccaaaat ggacgaattt agtagtcagt tgctggggta tttcacttta gcgctttata
    35101 ccagtgcccc ttctaaacta aagggagatc ttaactatct gcgactggag tgggggccag
    35161 attttcaaca gcatgaggca ggtttgatcg gtgctgacga agtgcccatt ctgacaacca
    35221 gttctgctga actggcacag caacagattg cgatgcttaa tggttgcacc tggctacccg
    35281 tcagctgggc gcgtaaaaaa ggcggcctgc ataccgttgt cgatagcaca acactttcac
    35341 ggccgcttta tgccatatgg ctgcaaaata gcgataaaaa tacgttgatt cgcgatctat
    35401 tgaaaattaa cgtgctggat gaagtgtatt aatctgaatg gctggcaagg atgccggtag
    35461 aaggatttac ttcagagagg gttatttcag ataaaaaaaa tccttagctt tcgctaagga
    35521 tgatttctgg caggggcgga gagactcgaa ctcccaacac ccggttttgg agaccggtgc
    35581 tctaccaatt gaactacgcc cctaattagg gtggcggaac ggacgggact cgaacccgcg
    35641 accccctgcg tgacaggcag gtattctaac cgactgaact accgctccac cgaattcttt
    35701 tacaaccacc ggttttatga ccggcttact gct
//
LOCUS       DAZFBR010000040        35477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFBR010000040 DAZFBR010000000
VERSION     DAZFBR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.040"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..689)
                     /locus_tag="ACHJZ2_002183"
     CDS             complement(<1..689)
                     /locus_tag="ACHJZ2_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO4568284.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGQVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKN"
     gene            complement(867..1778)
                     /gene="gsiD"
                     /locus_tag="ACHJZ2_002184"
     CDS             complement(867..1778)
                     /gene="gsiD"
                     /locus_tag="ACHJZ2_002184"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO4568285.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1781..2701)
                     /gene="gsiC"
                     /locus_tag="ACHJZ2_002185"
     CDS             complement(1781..2701)
                     /gene="gsiC"
                     /locus_tag="ACHJZ2_002185"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO4568286.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2719..4257)
                     /gene="gsiB"
                     /locus_tag="ACHJZ2_002186"
     CDS             complement(2719..4257)
                     /gene="gsiB"
                     /locus_tag="ACHJZ2_002186"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO4568287.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4277..6148)
                     /gene="gsiA"
                     /locus_tag="ACHJZ2_002187"
     CDS             complement(4277..6148)
                     /gene="gsiA"
                     /locus_tag="ACHJZ2_002187"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO4568288.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSELSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6135..7100)
                     /gene="iaaA"
                     /locus_tag="ACHJZ2_002188"
                     /pseudo
     CDS             complement(6135..7100)
                     /gene="iaaA"
                     /locus_tag="ACHJZ2_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            7304..8539
                     /gene="moeA"
                     /locus_tag="ACHJZ2_002189"
     CDS             7304..8539
                     /gene="moeA"
                     /locus_tag="ACHJZ2_002189"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO4568289.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8539..9288
                     /gene="moeB"
                     /locus_tag="ACHJZ2_002190"
     CDS             8539..9288
                     /gene="moeB"
                     /locus_tag="ACHJZ2_002190"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO4568290.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9467..10129)
                     /gene="fsa"
                     /locus_tag="ACHJZ2_002191"
     CDS             complement(9467..10129)
                     /gene="fsa"
                     /locus_tag="ACHJZ2_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO4568291.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            10260..11159
                     /gene="ybiY"
                     /locus_tag="ACHJZ2_002192"
     CDS             10260..11159
                     /gene="ybiY"
                     /locus_tag="ACHJZ2_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO4568292.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11165..13596
                     /gene="ybiW"
                     /locus_tag="ACHJZ2_002193"
                     /pseudo
     CDS             11165..13596
                     /gene="ybiW"
                     /locus_tag="ACHJZ2_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            13742..14557
                     /gene="ybiV"
                     /locus_tag="ACHJZ2_002194"
     CDS             13742..14557
                     /gene="ybiV"
                     /locus_tag="ACHJZ2_002194"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO4568293.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14709..15974
                     /gene="ybiU"
                     /locus_tag="ACHJZ2_002195"
     CDS             14709..15974
                     /gene="ybiU"
                     /locus_tag="ACHJZ2_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO4568294.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16142..17734)
                     /gene="ybiT"
                     /locus_tag="ACHJZ2_002196"
     CDS             complement(16142..17734)
                     /gene="ybiT"
                     /locus_tag="ACHJZ2_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO4568295.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17953..18873
                     /gene="ldtB"
                     /locus_tag="ACHJZ2_002197"
     CDS             17953..18873
                     /gene="ldtB"
                     /locus_tag="ACHJZ2_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4568296.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18931..20049)
                     /gene="ybiR"
                     /locus_tag="ACHJZ2_002198"
     CDS             complement(18931..20049)
                     /gene="ybiR"
                     /locus_tag="ACHJZ2_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO4568297.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20046..20513)
                     /gene="mntR"
                     /locus_tag="ACHJZ2_002199"
     CDS             complement(20046..20513)
                     /gene="mntR"
                     /locus_tag="ACHJZ2_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO4568298.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20699..20827
                     /gene="mntS"
                     /locus_tag="ACHJZ2_002200"
     CDS             20699..20827
                     /gene="mntS"
                     /locus_tag="ACHJZ2_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO4568299.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21099..22682
                     /gene="opgE"
                     /locus_tag="ACHJZ2_002201"
     CDS             21099..22682
                     /gene="opgE"
                     /locus_tag="ACHJZ2_002201"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO4568300.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22731..23246)
                     /gene="ompX"
                     /locus_tag="ACHJZ2_002202"
     CDS             complement(22731..23246)
                     /gene="ompX"
                     /locus_tag="ACHJZ2_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO4568301.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23299..23364)
                     /locus_tag="ACHJZ2_002203"
     CDS             complement(23299..23364)
                     /locus_tag="ACHJZ2_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568302.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23599..24486
                     /gene="rhtA"
                     /locus_tag="ACHJZ2_002204"
     CDS             23599..24486
                     /gene="rhtA"
                     /locus_tag="ACHJZ2_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO4568303.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24785..25288
                     /gene="dps"
                     /locus_tag="ACHJZ2_002205"
                     /gene_synonym="pexB"
     CDS             24785..25288
                     /gene="dps"
                     /locus_tag="ACHJZ2_002205"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO4568304.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25692..26438
                     /gene="glnH"
                     /locus_tag="ACHJZ2_002206"
     CDS             25692..26438
                     /gene="glnH"
                     /locus_tag="ACHJZ2_002206"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO4568305.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26576..27235
                     /gene="glnP"
                     /locus_tag="ACHJZ2_002207"
     CDS             26576..27235
                     /gene="glnP"
                     /locus_tag="ACHJZ2_002207"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO4568306.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27232..27954
                     /gene="glnQ"
                     /locus_tag="ACHJZ2_002208"
                     /pseudo
     CDS             27232..27954
                     /gene="glnQ"
                     /locus_tag="ACHJZ2_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
     gene            28071..30296
                     /gene="ybiO"
                     /locus_tag="ACHJZ2_002209"
     CDS             28071..30296
                     /gene="ybiO"
                     /locus_tag="ACHJZ2_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO4568307.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(30293..31219)
                     /gene="rlmF"
                     /locus_tag="ACHJZ2_002210"
     CDS             complement(30293..31219)
                     /gene="rlmF"
                     /locus_tag="ACHJZ2_002210"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO4568308.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            31495..31755
                     /gene="mcbA"
                     /locus_tag="ACHJZ2_002211"
     CDS             31495..31755
                     /gene="mcbA"
                     /locus_tag="ACHJZ2_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO4568309.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            32020..34302
                     /gene="fiu"
                     /locus_tag="ACHJZ2_002212"
     CDS             32020..34302
                     /gene="fiu"
                     /locus_tag="ACHJZ2_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO4568310.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            34344..35030
                     /gene="ybiX"
                     /locus_tag="ACHJZ2_002213"
     CDS             34344..35030
                     /gene="ybiX"
                     /locus_tag="ACHJZ2_002213"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO4568311.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            35104..35370
                     /gene="ybiI"
                     /locus_tag="ACHJZ2_002214"
     CDS             35104..35370
                     /gene="ybiI"
                     /locus_tag="ACHJZ2_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO4568312.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLR"
BASE COUNT         8703 a         9521 c         8820 g         8433 t
ORIGIN      
        1 tgattcttgg caatgatctc atggccttgt ttcaaaaata gaacttcatc attagcgctg
       61 atttgatcaa gatactcgtt cagatatcgc cagtttaaat cgacatgaat tttatccacc
      121 gctaacaaat cgtgatcttt gccgttgatg cgtagcgtaa aagagataca ttttttaccc
      181 ggacgatcaa agcaattgta ggccggattc cagctgttgt tagagtcatc tgaagccacc
      241 gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa cctgccctga ctcgtcgaca
      301 agcggtccat ttacttgttc ctgatgataa acaacgattt tatcgttcgc cctccgcgaa
      361 aagagataat atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt
      421 tccattttca ggatcacagc gcgttgaatc tcctctgtat tttttaataa atacagtgag
      481 tcctttaatg tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga
      541 ataagttcaa agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta
      601 atgatgaaaa aaaggccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt
      661 atctttatct tttcgtataa actcagcatg ttaatacatt taacaaaata actgtctgaa
      721 cgctgtgccg ctgcaaaaac agtcccttgc tgcggctatt taaccaaatt acccgattct
      781 acaaaatcag cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta
      841 ctctttcaag caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt
      901 aaaccatcgc ccaacaaatt gaacgccagt acagtcagaa aaatagccag ggccggaaaa
      961 acagcgacat gcggcgcgat aaccatatcc gctcgagcct cattgagcat tgctccccac
     1021 tctggtgtcg gcggctgcgc accgaggccg agaaatgaca ggctggcggc agagataatc
     1081 gaggtaccaa tgcgcatggt gaaaaacacc acgatagaag agacggtccc aggcaggata
     1141 tgacgcaaca aaatggtcat atcgctggca ccaatactgc gtgctgactc aataaaggtt
     1201 tgctgtttca acaccagcgt gttgccgcgc accaggcggg caaacgcagg gatggaaaaa
     1261 atggcgactg caataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc
     1321 gccagtaaaa tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc
     1381 caccagcctt cataatatcc agccaacaag cccagcaacg tccctatcgc cgcaccgata
     1441 aacacggcaa acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa
     1501 atgtcgcgcc ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc gttattcaga
     1561 ttgtcataat caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc
     1621 accacaatca ataaaataac gaataatgcg gcggtcatcg ccatatgctg acggcgaaat
     1681 cgtcgccaga attcatgcca cggtgtacgt acctggtcag gtttgaccag tggcatggcg
     1741 tttaacaccg cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg
     1801 ggttaatggc ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga
     1861 aaagcagaat ttccgcctga atcaccgggt aatcgcgcat ttctacggag tcaacgagta
     1921 agcgtccaag tccgggccag ttgaaaactt tctccaccac gatggaacca ccgagcaaaa
     1981 agccaaactg taagcccatc atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt
     2041 tgaggacaac ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc
     2101 ttaaaacgtc gacaaacgac gcgcgggtaa agcgcgccat cacggcggcc actgccgcgc
     2161 cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc tgctcccacg gtaggcagcc
     2221 agcccagttc aacggagaat acctgaatta aaagcatccc cagcgcaaat gccggaaacg
     2281 agatccccga caccgcaatg gtcatactca atcgatccgg ccaacggtta cgccagacgg
     2341 cggcgataat tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca
     2401 gccacagcgt tggcataaag cgcctggcaa tctcatcggc aaccggacga cgtgacacca
     2461 tcgacaggcc aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca
     2521 gcggctgatc caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcgg
     2581 gcccggcaat caatcgcgcc ggatcgccgg gcagcatatg cacaaataaa aacaccagca
     2641 ccgagacgat aaacagcgtc ggaatcaacc ccagtaatcg tttgataacg taattaagca
     2701 ttccactccc tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc cggtatctgg
     2761 catgatccaa aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag
     2821 cgggatccac ggcgactctt gccagatgat atcctgcgcc gccttatata agcgggtctt
     2881 ttccgccgga tcattagttt tcagtgcctg agccaggaag tcatccacct gtttattgct
     2941 gtaaaacgcg gtattaaaca gcgtcggtgg ccagttttgc gaggcaaaca acggcgatag
     3001 tgcccagtcc gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct
     3061 ctctttttgc cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcagtcac
     3121 ctgggcttta atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc
     3181 ggtgctgtgg ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctc
     3241 tttcagtaat tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta
     3301 ggcgatactc ggcggtacca caccagtagc tggcgttgca tagcccgcga aggccacttt
     3361 caccagcgcc ggacggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa
     3421 tggcttttgc gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa
     3481 ctcgatattt ttgtttttct ccagcagtgc ggcctgctcg taaggaatgg ggaaagcaaa
     3541 ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca
     3601 ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt
     3661 cacaaaatcg gtctgattcc aggtatccag ttcatacggt ccggttccca ccggatgaaa
     3721 accaatctcc ttgccatatt tttccagcgc tgccggtgaa atcatcgcgg tcgccggatg
     3781 ggcaagaata ttaataaacg ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg
     3841 atcgatcgct tccgttttag caatattctt atacaggtta tagcgtttaa gatgattcgc
     3901 cggatcgctg gcccggtcca gattcgcttt taccgccgct gcgttgaaat cggtgccatc
     3961 ctggaattta attccttccc gcaatttcac ggtgtaagta atgccgtcat cggaaacggt
     4021 ataactctcc gccagcacgt ttttcagttt catctcttta tccagaccga acagcccctg
     4081 gtaaaacgat ttcgctacgg cctgagataa cgtgtcattc gcgtcatacg gatcgagcgt
     4141 ggtgaaattc gatcctaccg ccaccaccac atctttggca gcgaatgcac aagatgccat
     4201 caacgctgtc gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt
     4261 attctccgcc tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt
     4321 aatgccccgg cccgacgcat tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa
     4381 tattgctggg aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag
     4441 cgaccggaac tgccgccagt aatttacgcg tataaggatg ctgcgggttt tcgaagaccg
     4501 cgcgccgtgg accaatttca acaatttgcc cgagatacat caccgccaca cgatgactaa
     4561 tccgctctac cacagccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct
     4621 ggagatcgag caacaagttg ataatctgcc cgcgaattga aacatccagc gccgaaacgg
     4681 cttcgtcggc aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct
     4741 ggcgctgacc gccggaaaac tcatgcgggt aacgccaggc atgttcaggt aacaggccca
     4801 cgcgctccag caaccacgca acgcgtgcaa ccgcttcttt acctggcaat aaaccgtgta
     4861 cgcgcagcgg ttcgataatc gaatcaccga tggtctgacg tgggtccagc gaagcgtaag
     4921 ggtcctgaaa aataaactga atatcgcggc gcaatgcctg aagtttgccg ggtgacaagg
     4981 tatcgattcg ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcagca
     5041 acgcccgccc ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc
     5101 caggccagag atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca
     5161 acaaaccgct gcgcaaaggg aaacgggtga ccagattacg cacccgtaaa acaggttcgc
     5221 catccaccac cgttttctgc tcgatggggg gggcctgttt cgctggatgt tcaagcgata
     5281 tcaatgggaa acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag
     5341 ctaacagcgc acgggtgtag ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg
     5401 tttccaccgc ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc
     5461 ccatatcgtg agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta
     5521 attgcaggat ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggctatca
     5581 gcactgccgg gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag
     5641 agagttgatg cggataacgt gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca
     5701 gcatccgctt cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaattg
     5761 attcggcaat ctgttcaccc acagtaaata ctgggttcag cgatgtcatc ggctcctgaa
     5821 atatcatcgc catatccgca ccgcgcacat ggcgcatttg tgcagcgctc agctcgctaa
     5881 gttcaatcac atcacgactg cgccgccgca acagcatttt atcgcactgc actaaaccgc
     5941 ccgcctgttc caacagacgc atcaatgcca gcgcagtcac tgacttaccg gagccggatt
     6001 cgccaacaat tgccagcgtc tcaccgcgtt gcagactaaa agagagattg cggaccgcag
     6061 ctattttctg ctggtcctgc ataaaggcaa tattaagatt ttcaaccgcc agcacattac
     6121 cggcatcaag ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg
     6181 gttggcgtat cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc
     6241 gcgacattcc cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt
     6301 ttttccatta ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac
     6361 gcggcgatgt catatgccgc cagcgcgcgg atgaagactt cgcccgtgcc ggtacaagaa
     6421 accgccacac tggcgttatt ggcgtagcat ccggcaccca ctaaggggct atcgccaact
     6481 cgtccgggta atttattggt cattccgccc gtggacgtgg ctgccgccag attgccgtct
     6541 aaatccaacg ccacggcccc cacggtgccc attttctgtt tttcatccag tggcgcaccg
     6601 ctatggtcga ggactgttgc cccttcctcg cgcgctgcca ttagttgttc ataacgcaaa
     6661 ggcgtggaga aaatctccgg tgagacgcgc tccatgccat gagcaaacgc aaaattttct
     6721 gccccttcgc caatcatcat cacatgcggg ctttactcca tcaccagccg ggcggcaaga
     6781 accggattac gcagatgact aacgcccgcc accgcaccgg ctttcagggt gttaccatcc
     6841 atcacacagg cgtccagttc atgcgtttca tcacgcgtaa agacggcgcc aattccggcg
     6901 ttaaacagtg gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt
     6961 tcgcccgctt ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag
     7021 cgtaattcct gttgcagact catctgcgcg cggctaattg cacctgcgcc accatgaatt
     7081 gcaatgactg ctttgcccat agtattcgtc cattaagggt ggctcgccac ttttgctata
     7141 aatatcagaa tttttatgaa ttactcattc aatttttgaa tatagaaaga tggatatggc
     7201 atgtaaaggc aggacccagc gctacgcccg aggtggcata cagtctgtgg cgtttttctg
     7261 acataatagg caaattcgat tttgcctccg caggagtgtt ttcatggaat ttaccaccgg
     7321 attgatgtcg ctcgacaccg cgcttaatga gatgctttct cgcgtcaccc cactgaccgc
     7381 ccaggaaacg ctgccactgg ttcagtgttt tggtcgtatt ctggcgagcg atgtcgtttc
     7441 gccgctggat gtcccagggt ttgataactc cgcaatggat ggttacgcgg tacgtttggc
     7501 cgatatcacc tccggacaac cgctgcccgt tgccggtaaa tcctttgccg gtcagccgta
     7561 ccatggtgaa tggcctgcgg gtacctgcat tcgtattatg accggtgcgc cggtgccgga
     7621 aggctgcgaa gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt
     7681 tactgctgaa gtgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg
     7741 tgcggttgtt ttcccggcgg ggactcgcct gactaccgca gagctgccag taattgcttc
     7801 actggggatt gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg
     7861 tgatgaactc cagttgccag gccagccgct gggcgacggc caaatctacg ataccaaccg
     7921 tctcgccgta cacctgatgt tagaacagct gggttgcgag gtaatcaact tagggattat
     7981 ccgcgacgat ccacatgccc tgcgcgccgc atttattgaa gccgacagcc aggcggatgt
     8041 ggtgatcagt tccggcggtg tttcagtggg tgaagcggat tacaccaaaa cgattcttga
     8101 agagctgggg gagatcgcct tctggaagct ggcgattaaa ccaggtaaac cgttcgcgtt
     8161 cggtaaactc agcaatagct ggttctgcgg cctgccgggc aacccggttt cagcgacgct
     8221 gaccttctat caactggtac agcctttgct ggcaaaacta agcggcaaca ccgccagcgg
     8281 cctgcccgcg cgccagcgcg tacgcacagc gtcccgcctg aaaaaaacgc caggacgtct
     8341 tgatttccag cgcggcgtgc tgcaccgcaa cgccgatggc gaactggaag tgacgaccac
     8401 cggacatcag ggttcacata tattcagctc ctttagcctc ggcaactgct ttatcgtgct
     8461 ggaacgcgat cgcggcaatg tggaagtggg cgaatgggtg gaagtagaac cgtttaacgc
     8521 gttgttcgga ggcctgtaat ggcggaactc agcgatcaag agatgctgcg ctacaaccgg
     8581 caaatcattc tgcgcggttt tgattttgac ggtcaggagg cgctgaaaga ttctcgtgtg
     8641 ctggtcgtcg gcctcggtgg actcggctgc gcagcttcgc agtatctggc aagcgccggt
     8701 gtcggcaacc tgacgctgct cgacttcgac tcggtttcgc tctcgaatct gcaacgccag
     8761 acgctgcaca gtgatgctac ggtcgggcaa ccgaaggtgg aatccgcccg tgacgctctg
     8821 acgcggatca acccacatat cgcgattacg ccagtcaatg cactgctgga tgacgcagaa
     8881 cttgcagcat tgattgctga acacaatctt gtgctcgact gtacggataa cgttgcggta
     8941 cgtaatcaac tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca
     9001 attcgtatgg aagggcaaat cacggtcttc acttatcagg acggcgaacc gtgctatcgc
     9061 tgccttagcc gtttgtttag cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca
     9121 ccgttgatcg gcgtgattgg ttcgttgcag gcaatggaag cgatcaaact gctggcaggt
     9181 tatggcaaac cggccagcgg gaaaatcgtc atgtacgatg cgatgacctg tcagtttcgc
     9241 gaaatgaaac tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca
     9301 ggcctgataa gacgcgacaa gcgtcgcatc aggcatttgc tccagacgcc agatgcggcg
     9361 tgaacgcctt atctggccta cggttcgggc acagatttcc aagcgcggca ggcatcggtg
     9421 cactccgcca cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc
     9481 tgccaaacgc tccctgccag tcctgctcaa acttcgccac agcggcatca accgccggat
     9541 agctaatcat ctgttgtgcc acatccagtg gcagagtaat tgattcacat cctgccagta
     9601 agcagtccag cgcctgacgc ggggttttga aactcgcggc taacactttc gcccgcggcg
     9661 catgcatttt caataactgg tgtaagtcgg tcacagtctg aataccgcta ccgccctgag
     9721 catcaatacg attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagcagcc
     9781 cttgtgctgc gccatatacc gcggttccca gcgtcggaat cccttccgct tttaacatct
     9841 taatagctgc cagcccctcg gtggtcaccg gaactttcac cacgatatcc gcaataatag
     9901 aacgcaattt acgtgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa
     9961 acagacgccc ctgaccgccc atcgcttcat gaagttccgg aagcacaacc tccagcggtt
    10021 ttttacccgc ggcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg
    10081 acagcgcctt caccgcaaca acgtctgaag tatccagata cagttccata accatcctca
    10141 aaatgtactt tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca
    10201 catcaatcta actttcattc gaaagtattt taatatttga acgaaacaga gaggcaacta
    10261 tgattttcaa tattcagcgc tactcgaccc atgatggccc cggtatccgc acggtcgtat
    10321 ttcttaaagg ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgca
    10381 cgcaggatct gctgtatgac ccacgtctgt gcctggaagg ctgtgagctg tgcgctaaga
    10441 ccgcgccgga agtgattgag cgcgcgctga atggtttgct tattcatcgg gaaaagttaa
    10501 ccccggagca tctgacggcg ttaaccgact gctgcccgac acagtcgtta accgtgtgtg
    10561 gggaagtgaa aagcgttgag gagatcatgg tgaccgttct gcgcgataaa ccgttttacg
    10621 atcgcagcgg cggcggctta acactttctg gcggtgaacc attcatgcag ccggaaatgg
    10681 cgacggcgct actgcaagcc agccacgagg ccggcattca tactgcggta gaaacctgcc
    10741 tgcatgtgcc gtggaaatat atcgcccctt ctctgcccta tatcgatctg tttcttgccg
    10801 atttaaaaca cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccaggg
    10861 tgctggataa cctgaaaaaa ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc
    10921 tgattcaagg ctttaatgcc gacgaaacct ctgtgaaagc cattaccgat tttgccgccg
    10981 acgagctgca cgttggcgaa attcatttcc tgccctacca cacgctgggc atcaacaaat
    11041 atcacttact taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagaactgc
    11101 tcgactttgc ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac
    11161 aaccatgacc acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc
    11221 gctggtgcat attgtgaaac cgccggtttg taccgagcgc gcgcagcact ataccgagat
    11281 gtatcaacaa catctcgata agccgatccc tgtacgtcgc gcgctggcgc tggcacatca
    11341 cctggcgaat cgcactatct ggatcaagca cgatgagttg atcatcggca accaggcaag
    11401 cgaagttcgc gccgcgccga tcttcccgga atatactgtc tcgtggatcg aaaaagagat
    11461 tgatgatctg gcagatcgtc ccggtgctgg ctttgcggtg agcgaagaga acaaacgcgt
    11521 tctgcatgaa gtgtgcccgt ggtggcgcgg tcagacggta caggatcgct gctacggcat
    11581 gtttaccgat gagcaaaaag gtctgctggc gaccggaatc attaaagcgg aaggcaatat
    11641 gacctccggc gatgcgcacc tggcggtgaa tttcccgctg ctgctggaaa aaggcttgat
    11701 ggtctgcgcg agaaagtagc ggaacgtcgc tcgcgcatca atctgacggt actggaagat
    11761 ctgcacggcg aacagttcct gaaagcgatt gatatcgtgc tggtggcagt cagtgaacac
    11821 attgaacgtt tcgctgccct ggcgcgtgca atggccgcga ccgaaacccg cgaaagccgt
    11881 cgcgatgaac tgctggcgat ggcagaaaac tgcgatctta tcgcccacca gccgccgcag
    11941 actttctggc aggcgctgca actgtgttac ttcatccagt tgattttgca gatcgaatct
    12001 aacggtcact cagtatcgtt tggtcgtatg gaccagtatc tctacccgta ctatcgccgc
    12061 gacgttgaac tcaaccagac actggatcgc gaacacgcca tcgaattgct gcatagctgc
    12121 tggctgaaac tgctggaagt gaacaaaatc cgctcaggct cacactcaaa agcctctgcg
    12181 ggaagtcctc tgtatcagaa cgtcaccatt ggcggacaaa atctggttga tggtcaacca
    12241 atggacgcgg tgaatccact ctcttacgcg atcctcgaat cctgcggtcg cctgcgttcc
    12301 actcagccta acctcagcgt gcgttaccac gcgggaatga gcaacgattt cctcgacgcc
    12361 tgcgtacagg tgatccgttg cggcttcggg atgccggcgt tcaacaacga cgaaatcgtc
    12421 atcccggaat ttattaaact cggtattgaa ccgcaggatg cttacaacta tgcagcgatt
    12481 ggttgtatcg aaaccgccgt cggtggcaaa tggggctatc gctgtaccgg catgagcttt
    12541 atcaacttcg cccgcgtgat gctggcggcg ctggaaggcg ggcgtgatgc caccagcggc
    12601 aaagtgttcc tgccacaaga aaaagcgttg tcggcaggta acttcaacaa cttcgatgaa
    12661 gtgatggacg cgtgggatac gcaaatccgt tactacacgc gtaaatcaat cgaaatcgaa
    12721 tatgtcgtcg acaccatgct ggaagagaac gtgcacgata ttctctgctc ggcgctggtg
    12781 gatgactgta ttgagcgagc gaaaagtatc aagcaaggcg gcgcgaaata tgactgggtt
    12841 tctggcctgc aggttggcat tgccaacctc ggcaacagcc tggcggccgt gaagaaactg
    12901 gtatttgaac agggcgcgat tagtcagcaa cagctggctg ccgcactggc gaatgacttc
    12961 gacggcctga ctcacgagca gctgcgtcag cggctgatta acggtgcgcc gaagtacggc
    13021 aacgacgatg atactgtcga tacgctgctg gctcgcgctt atcagaccta tatcgacgaa
    13081 ctgaaacagt accataaccc gcgctacggt cgtggtccgg ttggcggcaa ctattacgcg
    13141 ggtacgtcgt ctatctccgc taacgtaccg tttggcgcgc aaactatggc aacgccagac
    13201 ggacgtaaag cccacacccc gctggcagaa ggcgcaagcc cggcttccgg tactgatcat
    13261 cttggcccta ctgcggtcat tggctcggtg ggtaaactgc ctacggcagc gattctcggc
    13321 ggggtgttgc tcaaccagaa actgaatcca gcaacactgg agaacgaatc tgacaagcag
    13381 aaactgatga tcctgctgcg caccttcttt gaagtgcata aaggctggca tattcagtac
    13441 aacatcgttt cccgcgaaac gctgctggag gcgaaaaaac atcccgatca gtatcgcgat
    13501 ctggtagtgc gtgtcgcggg ttattccgcc ttcttcaccg cgctctctcc agacgctcag
    13561 gacgatatca tcgcccgtac cgaacatatg ctgtaatccc ctcagcccgg cggagacgtc
    13621 gccgggctaa atcattcaca tcatcaattt catccttact ttcattcgaa atataatttg
    13681 tgctctgcgt cacattgtta ttacactgtt acggtttcat tttcgagccg ggagcacacc
    13741 tatgagcgta aaagttatcg tcacagatat ggacggtact tttcttaacg acgtcaaaac
    13801 gtacaaccaa ccacgtttta tggcgcaata tcaggaactg aaaaagcgcg gcattgagtt
    13861 cgttgtcgcc agcggtaatc agtattacca gcttatttca ttctttcctg agctaaagga
    13921 tgagatctct tttgtcgcgg aaaacggcgc actggtttac gaacatggca agcaactgtt
    13981 ccacggcgaa ctgacccgac atgaatcgcg gattgttatt ggcgagttgc taaaagataa
    14041 gcaactcaat tttgtcgcct gcggtctgca aagtgcatat gtcagcgaaa atgcccccga
    14101 agcatttgtc gcactgatgg caaaacacta ccatcgcctg aaacctgtaa aagattatca
    14161 ggagattgac gacgtactgt tcaagttttc actcaacctg ccggatgaac aaatcccgtt
    14221 agtgatcgac aaactgcaca tagcgctcga tggcattatg aaacccgtca ccagtggttt
    14281 tggctttatc gacctgatta ttcccggtct acataaagca aacggtattt cgcggttact
    14341 gaaacgctgg gatctgtcac cgcaaaatgt ggtagcgatt ggcgacagcg gtaacgatgc
    14401 ggagatgctg aaaatggcgc gttattcctt tgcgatgggc aatgctgcgg aaaacattaa
    14461 acaaatcgcc cgttacgcta ccgatgataa taatcatgaa ggcgcgctga atgtgattca
    14521 ggctgtactg gataacacat ccccttttaa cagctgacct ctctaccgga gccatgcgct
    14581 ccggttatcc cttcttttta catcctgtac atcacatcaa atcattaaac ttgcattaac
    14641 ttatttataa cttatattgt cacatgcaaa tcagcatagt ttcgaatgaa agttaatgcg
    14701 aggtggttat ggcttctact tttaccagcg acacattgcc tgccgatcac aaagcagcta
    14761 tccgtcagat gaagcacgcg ctgcgggcgc agcttggcga cgtccagcag atctttaatc
    14821 agctaagcga tgacattgcc acgcgagtgg ctgaaatcaa cgcactcaaa gcacagggcg
    14881 atgccgtctg gccggtgctg tcttatgccg atatcaaagc aggtcatgtt actgcagagc
    14941 agcgcgaaca gattaaacgt cgcggttgtg cggtgataaa aggccatttc ccccgcgaac
    15001 aagcgctagg ctgggatcag tcgatgctgg actatctgga ccgcaaccgc tttgacgagg
    15061 tctacaaagg ccccggcgat aatttcttcg ggacgctcag cgcttcacgt cccgagattt
    15121 accccatcta ctggtcgcag gcgcaaatgc aggcccgcca gagtgaagaa atggcgaatg
    15181 cgcagtcgtt tctcaatcgt ctgtggacat ttgaaagtga tggaaagcaa tggtttaacc
    15241 cggatgtgag cgtcatctac cctgaccgta tccgccgccg tccgcccgga acgacctcca
    15301 aaggtcttgg agcgcatacc gactccgggg cgctggaacg ctggctgctt ccagcgtatc
    15361 agcacgtttt cgctaacgtc tttaatggca atctggcgaa atacgatccc tggcatgcgg
    15421 cacatcgtac ggaagttgaa gagtacacgg tggacaacac caccaaatgt tccgtgtttc
    15481 ggacattcca gggctggaca gcgctctctg atatgctgcc tagtcagggg ttgctgcacg
    15541 tcgtgcccat tcctgaagcc atggcgtacg tactgttacg tccgctgctt gatgatgtgc
    15601 cggaggatga actgtgcggc gtagcgcccg gaagagtgtt gccggtatca gagcaatggc
    15661 atccactgtt aattgaggcg ttaaccagca ttccaaaact cgaggccgga gactccgtct
    15721 ggtggcactg cgacgtcatc cattccgttg cccccgttga aaatcaacag ggttggggca
    15781 acgtgatgta cattcctgcg gcaccgatgt gcgagaaaaa tcttgcctac gcgcacaagg
    15841 tgaaggccgc actggaaaaa ggcgcatcgc cgggcgactt cccgcgcgag gactatgaaa
    15901 caaactggga aggacgcttt acgcttgccg acctcaacat tcacggtaag cgagcgttgg
    15961 gcatggatgt ttgagagtta attgaagtga attgatgtgg gattaagaaa atcgattcga
    16021 cgttactcag attgctgaca agtgcgcttt cttaatgcct gataagcata gcgcatcagg
    16081 caatcttgcg tttgtcatca gccgccgtca ccccgacagt atcggggtga cggataataa
    16141 cttactcgat ccctttacta cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc
    16201 gttccggggt gatttccaga atgcgggtcg ccagggagct tacgaactca cggtcgtgtg
    16261 aaacaaagat cagcgtgccc tgatacagtt ccagcgccat gttcagtgac tcgatggatt
    16321 ccatatccag gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta
    16381 acttaccaaa aagcatccgc cctttttctc caccggaaag cactttagca ggctttttga
    16441 tgtcgtcctg gctgaacagc aaacgaccga gaatactgcg caccgcctgc tcgtcatcgc
    16501 cttcctgctt ccactggctc atccattcga acacggtcag atcattttca aactcatatt
    16561 cgtggtcctg agcatagtaa ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt
    16621 ccggttgcag atcgcccacc agcgttttca acagcgttga tttaccgacg ccgttggtac
    16681 ccagtaccgc cagtttttca ccgacttcca gcagcaggtt gagattttta aacagcggac
    16741 cgttatcaaa ccctttggtc agaccttcca cttccagcgc gttacggaac agcttcttat
    16801 cctgttcaaa acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga
    16861 ttttatcaat ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa
    16921 agcggctaac gaaagattgc agctcagcaa tctgcgcttt cttcttggcg ttatcggcca
    16981 gcagacgttc acgcgcctgg gtcgccgccg tcatgtactc atcgtagtta cccggataaa
    17041 cgcgcagctc gccgtaatcc agatccgcca tgtgggtaca gaccatgtta aggaagtgac
    17101 ggtcgtgcga gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc
    17161 gaatggtgtc gatgtcgagg ttgttggtcg gttcgtcgag caggagaata tccggatcag
    17221 caaacagcgc ctgcgccagc aacacacgca gcttccagcc aggagcaact tcactcatcg
    17281 ggccgtagtg ctgttccact ggaattccca cgccaagcaa caattcaccg gcacgagctt
    17341 ccgcagagta accgtccatt tcgccgtatt taacttccag atcggccact ttatagccgt
    17401 cttcttcact catttccggc aaagcataga tgcggtcgcg ctcctgcttc acttcccaca
    17461 actctttatg ccccatgatc accgtatcca gcacagtgaa ctcttcaaag gcaaactgat
    17521 cctgacgcag tttaccaatg cgctcgttgg gatcgaggga aacgttaccc agcgtcggct
    17581 caaggtcgcc gccgaggatc ttcataaagg tggatttacc actaccgttc gcgccaatca
    17641 ggccgtaacg gttgccgccg ccaaatttga cggaaatgtt ttcaaacaac ggcttactgc
    17701 cgaactgcat ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac
    17761 aaaccgcgca ttatgccata agtctgaaag aagatcctgc tcctcgctta agatctacac
    17821 tcaagccaaa ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg
    17881 aacttataat gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa
    17941 atctcgctta acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc
    18001 ggcttttgca gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg
    18061 gttggtcaga atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt
    18121 gccgcggagt accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc
    18181 ttcctgccga aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc
    18241 gttcatgaag gcatcgtcat taacagtgca gagatgcgtc tgtattacta tccgaaaggg
    18301 accaacaccg ttatcgtgct gccgatcggc attggtcagt taggcaaaga tacgcctatc
    18361 aactggacca ccaaagttga gcgtaagaaa gcaggcccga cctggacgcc gaccgccaaa
    18421 atgcacgcag agtaccgcgc tgcgggcgaa ccgcttccgg ctgtcgttcc ggcaggtccg
    18481 gataacccga tggggctgta tgcactctac atcggtcgcc tgtacgctat ccatggcacc
    18541 aacgctaact tcggtatcgg tctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa
    18601 gacatcaaat tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag
    18661 ccggtaaaag cgaccaccga gccagacggc agccgttaca ttgaagtcca taacccgctg
    18721 tctaccaccg aagcccaatt tgaaggtcag gaaattgtgc caattaccct gacgaagagc
    18781 gtgcagacag tgaccggcca gccagatgtt gaccaggttg ttcttgatga agcgattaaa
    18841 aaccgctccg ggatgccggt tcgtctgaat taatcttcaa aacgtaaaac aaaggcggac
    18901 tcataatccg ccttttttat ttgccagacc ttaccaggcc gggatcataa ccaacaaaac
    18961 atatcccacc agcgccgccc acaacagcat cggtattgaa tagagatgga aacgccacca
    19021 gatgcggcga tcgttcgcca tacgtagcgc aatcaaattt gccagcgatc cgggtaataa
    19081 cccaaagcca cctacgttta ccgcccatgc cagtaataaa gacggcggca catagttcag
    19141 caacaatata gtactcggca cgttactgat cacctgcgat aaaccgattg ccgttaacca
    19201 taatccgggt tcagataaat gactcacgtt acccaacacg ccttgcaacg ctggaagctg
    19261 ggtcagtaaa tggacgtcga taaacatcgc cataaacacc agcagcagcg tccagtccac
    19321 actcaacacc acgcgacgcg ccagcagcgc aaagcccgcc gccacaatca ccagtcccca
    19381 cagctcttgt ttgaactcca gcgccgtcag aaagacgata tacagcccca gacaactcca
    19441 caccagccgc ggtttccact ccggtgtttg cacccccgta tggtattgga gtgcctttcc
    19501 agggaaacaa caccagcaca acagcaggag cgtcagcatc attgcgccag ccagcggtgc
    19561 catttgggca ataaatccgg caaacgaaag accagaacgt ccccagataa gaatattttg
    19621 cgggttgcca attggcgtca gtagcgaacc agcgttgact gccagcgcct caaaaataat
    19681 cagccgatta accgggatct cacacagcct ttttagcgtg atagtcagcg gaacaacaat
    19741 aaacagcgtg acatcgttgg tcagaaaggt agaaagcagc gccgccgcca gcaccataaa
    19801 catcgccagc cgacgctccg tagcaaagcg gcgcaccatt ttgcgcccca gcacatcaaa
    19861 ataaccgctt aactccacac ctttggtcag cagcatcagg ccgcttaagg tgatgatggt
    19921 gtgccagtcg atagcagcag gccaggattt cggtgcaaag ggcacgaaaa aacttaatcc
    19981 gataccaaca agaattaata actgaaaaaa acgatcgcct tgcagcgtgc gtaaaaaagg
    20041 caggctcatt tggcaccgtg tttctgggta aacaaacgaa aggcatccag cgtctcttca
    20101 ctaacatggt gctccatgcc ttccgcgtcg cgacgggcga tttccggact gacgcccaac
    20161 accaacaaga aattttcgac tatctgatgt cgctcgcggc tttcctgcgc cagcttctct
    20221 ccttctgccg ttaaaaacac gccacgccag gggatcattt caatcagccc catagttgcc
    20281 agtcgcttaa gcattttagc caccgtcggt tgcgaaactc ccaaacgagc agccatgtct
    20341 acctgacgag cttcccccac ttccctgatc aagtcagaaa tcagctcaac gtaatcatca
    20401 ataagctcgc gccgatgcgc ctctctgacc tggcggaacc cttcaacgtg ctcttcctcg
    20461 ttcactaact gcgtcacttt ttttgctgtt ggcgtacctg cgcgacgact cattgtgctt
    20521 cctcattatg gtgacgcata catagcgttc tcatttcgtg cgcacattgt aaaccagagt
    20581 tgcgaaggta caaaaaatta acgttttagc aatagctata taatatagcc tgtgctatat
    20641 ctgtatgtaa tgcaatcatc cctcaaggat cgacgggatt agcaagtcag gaggtcttat
    20701 gaatgagttc aagaggtgta tgcgcgtgtt tagtcattct ccctttaaag tacggttaat
    20761 gctgctctct atgttgtgcg atatggtcaa caacaaaccg cagcaagata aaccttccga
    20821 taaatagcgg cgtcgcggta cgccgcttca ctcctgcttt catgcaggca cgacgcgttt
    20881 tggtctgaaa aaccccactt tttgtcggat ttgcaatccc cttcgcaaaa gatttgttcg
    20941 tcagtagttg acctgaactg cggctcgctc tatcttcttg cagccctgcg tatattgcgg
    21001 ctcgcggatg cggacccctt tcccctcttc acgcactctt gcaggtattg acccttgacg
    21061 ccagggtaag cacatggcgt ttgttacgat agtggcatat gaatttaacc ctcaaagaat
    21121 cgcttgttac ccgtagccgg gtatttagcc cgtggactgc gttctacttt ttacagtcgc
    21181 tattaattaa cctcggctta ggttacccct tcagtttgct ctacaccgct gcgtttacgg
    21241 ctattttgct tttgctatgg cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca
    21301 gttcgctggt ggcggcttgt tatttccctt ttgctcaggc ctacggcgcg cctaatttca
    21361 atacattgct ggcattgcac tccaccaata tggaagagtc gaccgaaatc ctgacgattt
    21421 ttccgtggta cagctacctg gtcggcttat ttatttttgc gctcggcgta atagcaatca
    21481 ggcgaaaaaa agagaatgaa aaagcgcgct ggaatacctt cgacagcctg tgtctggtat
    21541 tcagtgtggc gacatttttt gttgctcccg tgcaaaacct ggcctggggt ggcgtattta
    21601 aactgaaaga tactggctat ccggtatttc gttttgctaa ggatgtcatc gtcaataata
    21661 acgaggtgat tgaagagcaa gaacggatgg caaaactttc cggaatgaaa gatacctgga
    21721 cggtcactgc cgttaagccg aagtatcaga cctatgtggt ggtgatcggt gaaagcgcgc
    21781 gtcgcgatgc cctcggtgcc tttggcggtc actgggacaa taccccgttt gccagcagcg
    21841 ttaacggttt gatatttgct gactacattg ccgccagtgg ctccacgcag aaatcgcttg
    21901 gcttaacgct caatcgcgta gtcgatggca aaccacagtt tcaggataac tttgtcaccc
    21961 tggcaaatcg cgcgggcttc cagacctggt ggttttccaa ccagggccaa atcggcgaat
    22021 acgatactgc tatcgccagc atcgccaaac gtgcagatga agtgtacttc ctgaaagaag
    22081 gtaattttga agcagataaa aacacgaaag acgaagcgtt actggatatg accgctcaag
    22141 tgctggcgca agagcactcg caaccgcagc tgattgttct gcatctgatg ggctcgcatc
    22201 cgcaggcctg cgaccggaca aaaggcaaat acgaaacctt tgtgcaatcg aaagaaacgt
    22261 cgtgctatct ctatactatg acgcaaacgg acgatttact gcgcaagctg tacgatcagt
    22321 tacgcaacag cggcagcagc ttctcgctgg tttacttttc tgaccacggt ctggccttta
    22381 aagagcgcgg caaagacgtg caataccttg cccatgatga taagtatcag caaaatttcc
    22441 aggtgccttt tatggtcatt tccagcgacg ataaagcgca tcgggtgatt aaagcccgcc
    22501 gctcagccaa tgacttctta ggctttttct cgcagtggac gggaattaaa gcgaaggaaa
    22561 ttaacatcaa atacccgttt atatctgaga agaaagccgg gccgatatac atcaccaact
    22621 tccagttaca gaaggtggat tacaaccatc tcggaaccga tattttcgac ccgaagcctt
    22681 aaaaacaaaa atccgccccg agaggcggat tttttatatc accaaagtga ttagaagcgg
    22741 taaccaacac cggcaatcca ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa
    22801 gagaagtcca gagcaacgtt ttccatcggg ttgaactgca gaccagcacc gtaggagaaa
    22861 ccgtagtcgc tggtgtcgtg tttgtaggtc gggtattcag tggtctggaa tttaccataa
    22921 cccacaccca ctacaccgta gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca
    22981 gtgatgccgt agtactggtt tttgttgtag tcaccagagc ttgcagtacg gcttttctcg
    23041 gtgtaagtga atgaaccgat cacacccagc gggctgttgt cttcttcata gcggtatttc
    23101 aggttgaaac cgcccatttt gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca
    23161 gttacggtag aagtcgcagc tacggaagta cctgcggtga aagccagaac tgcggccagt
    23221 gctgaaagac atgcaatttt tttcataacc acctcaaatg tgattcaaat aagtcctaag
    23281 ttttaaatat atcaaaaatt aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca
    23341 tttcatgtaa cagaacgttt ccatacaccg ctatccatct aaatttaaat cactttttca
    23401 gagaactgcg taagtattac gcatgttttc cctgtcattc atccagatta ttcctaatca
    23461 ccagactaat gattccatca atcctggcgc attttagtca aaacggggga aaattttttc
    23521 aacaaatgct caaccagcat tgggtatatc cagtacactc cacgctttac ttaagtctag
    23581 atatttgtgg gagaaaggat gcctggttca ttacgtaaaa tgccggtctg gttaccaata
    23641 gtcatattgc tcgttgccat ggcgtctatt caaggtggag cttcgttagc taagtcactt
    23701 tttcctctgg tgggcgcacc gggtgtcact gcgctgcgcc tggcattagg aacgctgatc
    23761 ctcatcgcgt tctttaagcc atggcgactg cgctttgcca aagagcaacg gttaccgctg
    23821 ttgttttacg gcgtttcgct gggtgggatg aattatcttt tttatctctc tattcagacg
    23881 gtaccgctgg gtattgcggt ggcgctggag ttcaccggac cactggcggt ggcgctgttc
    23941 tcttctcgtc gcccggtaga tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc
    24001 ctgctaccgc tggggcaaga cgtttcccat gtcgatttaa ccggctgtgc gctggcactg
    24061 ggggccgggg cttgttgggc tatttacatt ttaagtgggc aacgcgcagg agcggaacat
    24121 ggccctgcga cggtggcaat tggttcgttg attgcagcgt taattttcgt gccaattgga
    24181 gcgcttcagg ctggtgaagc actctggcac tggtcggtta ttccattggg tctggcggtc
    24241 gctattctct cgaccgctct gccttattcg ctggaaatga ttgccctcac ccgtttgcca
    24301 acacggacat ttggtacgct gatgagcatg gaaccggcgc tggctgccgt ttccgggatg
    24361 attttcctcg gagaaacact gacacccata cagctgctgg cgctcggcgc aattatcgcc
    24421 gcttcaatgg gttctacgct gacagtacgc aaagagagca aaataaaaga attagacatt
    24481 aattaaattt acatttctgc atggttatgc ataaccatgc agaatttctc gctacttttc
    24541 ctctacaccg tctttatata tcgaattatg caaaagcata tttattccga aaattcctgg
    24601 cgagcaaata aataagaatt gttcttatca atatatctaa ctcattgaat ctttattagt
    24661 tttgtttttc acgcttgtta ccactattag tgtgatagga acagccagaa tagcggaaca
    24721 catagccggt gctatactta gtctcgttaa ttactgggac ataacatcaa gaggatatga
    24781 aattatgagt accgctaaat tagttaaatc aaaagcgacc aatctgcttt atacccgcaa
    24841 cgatgtctct gacagcgaga aaaaagcaac agtagagttg ctgaatcgcc aggttatcca
    24901 gtttattgat ctttctttga ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt
    24961 cattgccgta catgaaatgc tggatggctt ccgcactgca ctgatcgatc atctggatac
    25021 catggcagaa cgtgcagtgc agctgggcgg tgtagctctg gggaccactc aagttatcaa
    25081 cagcaaaacc ccgctgaaaa gttacccgct ggacatccac aacgttcagg atcacctgaa
    25141 agaactggct gaccgttacg caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc
    25201 gaaagatgac gacaccgcag atatcctgac cgccgcgtct cgcgacctgg ataaattcct
    25261 gtggtttatc gagtctaaca tcgaataaat ccatcgctga tggtgcagaa ttttagtacc
    25321 cgataaaagc ggcttcctga caggaggccg ttttgttttg cagcccgcct caacgcactt
    25381 atttagtgca tccatctgct atccccagct gattaagtaa attttttgta tccacatcat
    25441 cacacaatcg ttacataaag attgtttttt catcaggttt tacgctaaat aatcactgtg
    25501 ttgagtgcac aattttagcg caccagtttg gtgccccaga atggtgcatc ttcagggtgt
    25561 tgccctataa atcgtgcatc acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa
    25621 ctggcacgat tttttcatat atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa
    25681 aaggaaatgc tatgaagtct gtattaaaag tttcactggc tgcactgacc ctggcttttg
    25741 cggtttcttc tcatgccgcg gataaaaaat tagttgtcgc gacggatacc gccttcgttc
    25801 cgtttgaatt taaacagggc gataaatatg tgggctttga cgttgatctg tgggctgcca
    25861 tcgctaaaga gctgaagctg gattacgaac tgaagccgat ggatttcagt gggatcattc
    25921 cggcactgca aaccaaaaac gtcgatctgg cgctggcggg cattaccatc accgacgagc
    25981 gtaaaaaagc gatcgatttc tctgacggct actacaaaag cggcctgtta gtgatggtga
    26041 aagctaacaa taacgatgtg aaaagcgtga aagatctcga cgggaaagtg gttgcggtga
    26101 agagcggcac tggctccgtt gattacgcga aagcaaacat caaaactaaa gatctgcgtc
    26161 agttcccgaa catcgataac gcctatatgg aactgggcac caaccgcgca gacgccgttc
    26221 tgcacgatac gccaaacatt ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag
    26281 cggtaggcga ctctctggaa gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg
    26341 agctgcgtga caaagtcaac ggcgcgttga aaaccctgcg cgagaacgga acttacaacg
    26401 aaatctacaa aaaatggttc ggtactgaac cgaaataata acgctacacc tgtaaagcgc
    26461 actggcagtt ccctctcccc tctggggaga ggattagggt gagggcgcaa acccgctccg
    26521 gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac aacatatgca
    26581 gtttgactgg agtgctatct ggcctgccat tccgcttctg attgaaggtg ccaaaatgac
    26641 cctgtggata tcggtcctcg gtctggctgg cggcctggtg atcggattgc tggcaggttt
    26701 tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggttttta ttgaagtgat
    26761 ccgcggcaca cctatcgtcg tccaggtgat gtttatctat tttgccctgc cgatggcgtt
    26821 taatgactta cgcatcgacc catttactgc ggcggtggtc accatcatga tcaactccgg
    26881 cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga
    26941 agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca ttttaccgct
    27001 ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca ttaaagacac
    27061 ctcgctgttt attgtgatcg gcgtggcgga actgacccgt caggggcaag aaattattgc
    27121 cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgttctatc tgattattac
    27181 cctggtgctg agctttattc tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa
    27241 tttaaaaacg tctccaagca ctttggccca acccaggtgc tgcacaatat cgatttgaac
    27301 attgcccagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa atcgaccctg
    27361 ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt cgatggcctg
    27421 aaggttaacg atccgaaagt tgacgagcgc ctgattcgct aggaagcagg tatggtgttc
    27481 cagcagtttt acctcttccc gcatctgacg gcgctggaaa acgtcatgtt tggcccgcta
    27541 cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cacgtgagct gctggcgaaa
    27601 gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg tcaacagcag
    27661 cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg
    27721 acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca agatctggct
    27781 gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga gaaagtagct
    27841 tcgcgcctga tctttatcga caaaggccgg attgcggaag atggcgatcc acaagtgttg
    27901 attaaaaatc cgccgagcca gcgcttgcag gaatttttac agcacgtctc ttaacttctc
    27961 tgcctccctt tcacccgaaa gggaggtaca ccagattcct ctcatttaaa atcacccctc
    28021 ccccagcatc tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga
    28081 tcctgttcat cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg
    28141 ttacaaccac aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac
    28201 aaaaaaaagc ggcttatggc gcactggcgg atgtgctgga taatgacacc tcgcgtaaag
    28261 agttgatcga ccagttgcgc accgtcgccg cgacgccacc tgctgaaccg gtcccgaaga
    28321 tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaattcagcc
    28381 gccattatgg tgaagccctt tccgcccgct tcgggcagct ttatcgcaat atcaccggct
    28441 ccccgcataa gccgtttaat ccacaaacct tcagcaatgc gctgacccat ttttcaatgt
    28501 tagcggtttt agtgtttggt ttttactggc tgattcgcct gtgcgcactg ccgctgtatc
    28561 gcaaaatggg tcagtgggcg cggcaaaaaa atcgtgagcg cagtaactgg ctgcagcttc
    28621 cggcgatgat tatcggggcg tttattatcg acctgctgtt actggcactg acattatttg
    28681 tcggccagat attaagcgac aacctgaatg caggcagtcg caccatcgct ttccaacaaa
    28741 gtttgtttct caacgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt
    28801 tttgcccaaa cgtggcggaa ctgcgcccgt tcacgattca ggacgagagc gcccgttact
    28861 ggagtcgtcg cctgagctgg ttaggcagtt tgataggtta tggcctgatt gtggccgtgc
    28921 cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc
    28981 tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa taaaaaagag attacccagc
    29041 atttgctcaa ctttgcggag cattcgctgg cctttttcag cctgtttatc cgcgcctttg
    29101 cgctggtgtg gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt
    29161 tcgatccggg caacagcctg aaatttatga tgggtgcaac agtgcgcagc ctggcgatta
    29221 ttggtatcgc agcatttgtt tccggtatgt tttcccgctg gctggcaaaa accatcactc
    29281 tctcgccaca tactcagcgt aactatccgg agttgcaaaa acggctgaat ggctggcttt
    29341 cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcggtaatg ctgctgttga
    29401 gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg
    29461 tagatatcct gatccgtatc gcactcattc ttttcttctc ggcggttggc tggacggtgc
    29521 tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg
    29581 cccgcacgcg taccctgctt acgctgtttc gtaacgcgct ggcggtgatt atcagtacca
    29641 tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg
    29701 ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtgaaa gatattatca
    29761 ccggggtatt tattcagttt gaaaacggca tgaacactgg agatttggtg actattgggc
    29821 cgttgaccgg cactgtggaa cggatgtcga ttcgctccgt gggcgtacgc caggataccg
    29881 gggcgtacca catcattccg tggtcttcga ttaccacctt tgccaacttt gtccgcggca
    29941 ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc
    30001 aggcactgaa agatgcggta gcggaattaa tggaaaacga agaaattcgt gggctgatta
    30061 ttggtgaacc gaactttgcc gggattgttg gcttaagcaa taccgcgttt acactgcgtg
    30121 tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc gacagccagg
    30181 tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccggt gcagacttat caggtgctgc
    30241 ctgctccggg cgcgaccccg gctgaaccgt taccgccggg ggagccaacg ctttaacgct
    30301 ggcgattgac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac
    30361 tctgcttttg cccctgggcc atctcttttt taaccacctt caccgcgccc acgtccgtca
    30421 gggcacgata caacggcggt aagttttcac cacgagatac cagtgaagta aaccacatca
    30481 cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaaggtg acctcaccgc
    30541 cttcacacca caactcctgt tgctggccgc caaagttcag tgcatcgtct ttgttcagcc
    30601 ccaggttacg gcgtttacgc tcactacctg cccgcgccgc agcggcggaa tcgtggaatg
    30661 gcgggttaca caaggtcgcg tcgtattgct cgtttttatg gatgatgccg ttaaaaatcg
    30721 ccccactctc tttttgccga cgcagacgaa tggcgcgatt aagccccgga ttagcactga
    30781 tgatcgcctg cgcgctgctt aacgcctggc tgctggtttc actaccggta aaacgccagc
    30841 catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg atatcaagaa
    30901 tgctggcatt agctggaatt gttccgcttg cttcagccag taaatcggca aggtgatgaa
    30961 tataatccgc ccggcccggt actggcgggc agagaaaacc gtccgggata tcccagttcg
    31021 ctacggcgta aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc agcggattgg
    31081 caaagtctac gctttgctcc ccggcgggtg taagcgtgag gaattgcttg agttcaggat
    31141 tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc
    31201 ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac
    31261 gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt
    31321 ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttgatttgtc ttattttgta
    31381 aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca tttgcatcgc
    31441 gcaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa
    31501 aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc
    31561 gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca
    31621 actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgagcaa
    31681 ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt cggtactgca
    31741 accatctaca aataacccca ccaacgtaac tgcattcgcc tctggatgca gttttcgcct
    31801 gttttgtaat caaaaaacat caaatatgaa cgcaatgtaa ataaatgtat ttctttttcg
    31861 cgcaatgggt gatagaaaat cgcgccaagt gataatgctt atcaaaatta ttatcacttg
    31921 cacgaacact atcacgagat taacagtggc atcgcatccg cagagaggct ttctcgtggc
    31981 agtgaaaatt tcaacatata agaaaaagtc acctgcaaaa tggaaaacaa tcgcaatttc
    32041 cctgccagac aatttcattc gctcacgttc tttgccggtc tttgtattgg catcacgcct
    32101 gtggctcagg cactcgccgc cgaagggcaa actaacgcgg atgacacgct ggttgtcgaa
    32161 gcatcaacgc cttcgcttta tgcgccacaa caatctgccg atccgaaatt ctcgcgtccg
    32221 gtagcggata ctacccgcac gatgacggtg atttctgaac aagtgattaa agatcagggc
    32281 gcaaccaacc ttaccgatgc gctcaaaaac gtccccggcg tgggtgcgtt ttttgcgggt
    32341 gagaacggta actccaccac tggcgacgcc atttatatgc gcggtgctga tacctctaac
    32401 agtatttata ttgatggcat tcgcgatatc ggcagcgtct cgcgcgacac cttcaatact
    32461 gagcaggtcg aagtgattaa agggccttcc ggcaccgact acgggcgcag cgcgccgacg
    32521 ggctcgatca atatgatcag caagcagccg cgcaatgatt ccggcattga cgcctccgcc
    32581 agtattggca gcgcctggtt ccgccgcggc acgctggacg tcaatcaggt cattggtgat
    32641 accactgcgg tgcgcctgaa tgtgatgggc gaaaaaacgc acgatgccgg acgcgacaaa
    32701 gtcaaaaatg agcgttacgg cgtcgcccct tctatcgctt ttggccttgg tacagcgaat
    32761 cgtttgtatc ttaattatct gcatgtcacc cagcacaaca cgccagacgg cggcattcca
    32821 accatcggtt tgccgggcta ttctgcccca tctgcaggaa cggcgaccct gaatcattcc
    32881 ggaaaagttg atacgcataa cttttacggc acggattccg attacgacga ttcgaccacc
    32941 gacaccgcca ccatgcgttt tgagcacgat atcaacgata acaccaccat tcgcaacact
    33001 acccgttggt cgcgcgtgaa gcaggattac ctgatgacgg cgattatggg tggggcgtcg
    33061 aatattaccc agcccaccag cgatgtgaat agctggacgt ggtcacgcac ggcgaatacc
    33121 aaagatgtga gtaataaaat tctcaccaac cagaccaacc tgacctcgac gttctatacc
    33181 gcttctatcg gtcatgatgt cagtaccggc gtggaattta cccgtgaaac gcagactaac
    33241 tacggcgtta atccggtgac gttacctgcg gtaaatattt atcatcctga cagcagcatt
    33301 catcccggcg gcctgacgcg caacggcgca aacgccaatg gtcagacgga taccttcgca
    33361 atttacgcat tcgatacgct gcaaatcacc cgtgattttg agctgaacgg cgggatccgt
    33421 ctggataatt atcatactga atatgacagt gccaccgcct gcggcggcag cggacgcggt
    33481 gccatcacct gcccagctgg tgtggcaaaa ggttctccgg tcaccaccgt cgacaccgcc
    33541 aagtcgggca atctggtgaa ctggaaagcc ggggcgctgt atcacctgac ggaaaacggc
    33601 aatgtctata ttaactatgc cgtttcccag cagcctccgg gcggcaacaa cttcgccctt
    33661 gcgcagtctg gcagcggtaa cagtgccaac cgcaccgatt ttaaaccgca aaaagccaac
    33721 accagcgaga ttggcaccaa atggcaggtt ctggataaac gcctgttgct caccgccgcg
    33781 ctgttccgta ctgatatcga aaatgaagtt gagcaaaatg atgacgggac ttactcgcaa
    33841 tacggtaaga aacgcgtcga aggctatgag atatccgtgg cagggaatat cactcccgcg
    33901 tggcaggtga ttggcggcta tacccagcaa aaagcaacca tcaaaaacgg caaagatgtt
    33961 gcccaggatg gttcctcatc gctgccgtat accccggagc acgccttcac cttatggagc
    34021 caatatcagg caaccgatga tatctctgtt ggcgcgggcg cacgctatat cggcagtatg
    34081 cataaaggtt cagacggcgc ggtgggaacg ccagcgttta ccgaaggtta ctgggtcgcc
    34141 gatgccaaac tggggtatag ggttaatcgc aatctcgact tccagctaaa cgtctacaac
    34201 ctgtttgata ccgattacgt cgcctcaatc aataagagcg gctaccgtta tcacccgggc
    34261 gagccaagaa ccttcttgct cacagccaat atgcatttct gattcagatg tggggcgcag
    34321 gccccacttt ttggagaact tgtatgatgt accacattcc cggcgtgtta tcgccacagg
    34381 acgtcgcccg ttttcgcgaa caactggaac aagccgaatg ggtggatgga cgcgtcacca
    34441 ccggcgcaca aggtgcgcaa gttaagaaca atcaacaggt agacacccgc agcacgttat
    34501 acgccgccct gcaaaatgag gtgctgaacg cggttaacca acatgctttg ttctttgccg
    34561 cggccttgcc gcgtaccctt tccacgccgc tgtttaatcg ctatcagaac aatgaaacct
    34621 atggttttca tgtggatggc gcagtacgca gccatccaca aaacggctgg atgcgcactg
    34681 acctttctgc cacgctgttt ttaagcgatc cacaaagcta cgacggcggc gaactggtcg
    34741 ttaatgacac cttcggacaa catcgggtaa aactcccggc aggtgatctg gtgttgtatc
    34801 cctccagcag cctgcattgc gtgacacccg taacccgcgg cgtacgagtg gcatcattta
    34861 tatggatcca gtcgatgatc cgcgatgata aaaagcgcgc catgctgttt gaactggaca
    34921 aaaatattca gaatattcag tcgctgaaaa gccgctatgg tgaaaatgaa gagatcctgt
    34981 cgctgctcaa tctttatcat aatctgctgc gggaatggtc ggagatctga cgcttgaact
    35041 gcccaggttc gtccctatat tagctacact taactataca agcattgata tggggaggac
    35101 gatatggcat ccggttgggc taacgatgac gccgtcaacg aacagatcaa cagtacaatt
    35161 gaagatgcga ttgcccgcgc tcggggtgaa attccgcgtg gcgaaagcct gtatgaatgt
    35221 gaagagtgcg gtgcccccat cccgcaagcc cgtcgggaag ccattcctgg cgtgcgctta
    35281 tgtattcatt gtcagcagga gaaagattta caaaaaccag cttatacagg atataatcgc
    35341 agaggttcga aagaaagcca gttacgttaa ctacaactgg cgagatgtat agcgagtata
    35401 atttgtattt tgcgtggcat tccgtgcctt taacgctgtc gtgactgcca cgctgcataa
    35461 aacaagtcca taagtac
//
LOCUS       DAZFBR010000041        34601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFBR010000041 DAZFBR010000000
VERSION     DAZFBR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.041"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..633
                     /locus_tag="ACHJZ2_002215"
     CDS             <1..633
                     /locus_tag="ACHJZ2_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568313.1"
                     /translation="AVNHFRYKFLLSGNAGEMILYFANVFFNPLALSFIIATIICITI
                     KKRSSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGITVTVPSKQWVANIVK
                     QGPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHVCDVQGFACAYQEGV
                     QTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKMMLSARQ"
     gene            756..1382
                     /locus_tag="ACHJZ2_002216"
     CDS             756..1382
                     /locus_tag="ACHJZ2_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568314.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(1421..1666)
                     /gene="zapB"
                     /locus_tag="ACHJZ2_002217"
     CDS             complement(1421..1666)
                     /gene="zapB"
                     /locus_tag="ACHJZ2_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO4568315.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            2092..2937
                     /gene="glpF"
                     /locus_tag="ACHJZ2_002218"
     CDS             2092..2937
                     /gene="glpF"
                     /locus_tag="ACHJZ2_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO4568316.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            2960..4468
                     /gene="glpK"
                     /locus_tag="ACHJZ2_002219"
     CDS             2960..4468
                     /gene="glpK"
                     /locus_tag="ACHJZ2_002219"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO4568317.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4698..5708
                     /gene="glpX"
                     /locus_tag="ACHJZ2_002220"
     CDS             4698..5708
                     /gene="glpX"
                     /locus_tag="ACHJZ2_002220"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO4568318.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            5805..6551
                     /gene="fpr"
                     /locus_tag="ACHJZ2_002221"
     CDS             5805..6551
                     /gene="fpr"
                     /locus_tag="ACHJZ2_002221"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO4568319.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(6556..6984)
                     /gene="uspD"
                     /locus_tag="ACHJZ2_002222"
     CDS             complement(6556..6984)
                     /gene="uspD"
                     /locus_tag="ACHJZ2_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO4568320.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(7011..7310)
                     /gene="yiiS"
                     /locus_tag="ACHJZ2_002223"
     CDS             complement(7011..7310)
                     /gene="yiiS"
                     /locus_tag="ACHJZ2_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO4568321.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(7522..7962)
                     /gene="yiiR"
                     /locus_tag="ACHJZ2_002224"
     CDS             complement(7522..7962)
                     /gene="yiiR"
                     /locus_tag="ACHJZ2_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4568322.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            8063..8662
                     /gene="yiiQ"
                     /locus_tag="ACHJZ2_002225"
     CDS             8063..8662
                     /gene="yiiQ"
                     /locus_tag="ACHJZ2_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO4568323.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            8770..9537
                     /gene="tpiA"
                     /locus_tag="ACHJZ2_002226"
     CDS             8770..9537
                     /gene="tpiA"
                     /locus_tag="ACHJZ2_002226"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO4568324.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(9592..10347)
                     /gene="cdh"
                     /locus_tag="ACHJZ2_002227"
     CDS             complement(9592..10347)
                     /gene="cdh"
                     /locus_tag="ACHJZ2_002227"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO4568325.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(10454..11443)
                     /gene="sbp"
                     /locus_tag="ACHJZ2_002228"
     CDS             complement(10454..11443)
                     /gene="sbp"
                     /locus_tag="ACHJZ2_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO4568326.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(11762..12724)
                     /gene="pfkA"
                     /locus_tag="ACHJZ2_002229"
     CDS             complement(11762..12724)
                     /gene="pfkA"
                     /locus_tag="ACHJZ2_002229"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO4568327.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(12905..13807)
                     /gene="fieF"
                     /locus_tag="ACHJZ2_002230"
     CDS             complement(12905..13807)
                     /gene="fieF"
                     /locus_tag="ACHJZ2_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO4568328.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(13956..14456)
                     /gene="cpxP"
                     /locus_tag="ACHJZ2_002231"
     CDS             complement(13956..14456)
                     /gene="cpxP"
                     /locus_tag="ACHJZ2_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO4568329.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            14606..15304
                     /gene="cpxR"
                     /locus_tag="ACHJZ2_002232"
     CDS             14606..15304
                     /gene="cpxR"
                     /locus_tag="ACHJZ2_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO4568330.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            15301..16674
                     /gene="cpxA"
                     /locus_tag="ACHJZ2_002233"
     CDS             15301..16674
                     /gene="cpxA"
                     /locus_tag="ACHJZ2_002233"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO4568331.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(16780..17454)
                     /gene="yiiM"
                     /locus_tag="ACHJZ2_002234"
     CDS             complement(16780..17454)
                     /gene="yiiM"
                     /locus_tag="ACHJZ2_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO4568332.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(17603..18586)
                     /gene="kdgT"
                     /locus_tag="ACHJZ2_002235"
     CDS             complement(17603..18586)
                     /gene="kdgT"
                     /locus_tag="ACHJZ2_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO4568333.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(18846..19466)
                     /gene="sodA"
                     /locus_tag="ACHJZ2_002236"
     CDS             complement(18846..19466)
                     /gene="sodA"
                     /locus_tag="ACHJZ2_002236"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO4568334.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            19751..20785
                     /gene="rhaT"
                     /locus_tag="ACHJZ2_002237"
                     /pseudo
     CDS             19751..20785
                     /gene="rhaT"
                     /locus_tag="ACHJZ2_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(20782..21630)
                     /gene="rhaR"
                     /locus_tag="ACHJZ2_002238"
     CDS             complement(20782..21630)
                     /gene="rhaR"
                     /locus_tag="ACHJZ2_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO4568335.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(21704..22540)
                     /gene="rhaS"
                     /locus_tag="ACHJZ2_002239"
     CDS             complement(21704..22540)
                     /gene="rhaS"
                     /locus_tag="ACHJZ2_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO4568336.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            22828..24301
                     /gene="rhaB"
                     /locus_tag="ACHJZ2_002240"
                     /pseudo
     CDS             22828..24301
                     /gene="rhaB"
                     /locus_tag="ACHJZ2_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
     gene            24298..25557
                     /gene="rhaA"
                     /locus_tag="ACHJZ2_002241"
     CDS             24298..25557
                     /gene="rhaA"
                     /locus_tag="ACHJZ2_002241"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO4568337.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            25732..26556
                     /gene="rhaD"
                     /locus_tag="ACHJZ2_002242"
     CDS             25732..26556
                     /gene="rhaD"
                     /locus_tag="ACHJZ2_002242"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO4568338.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            26566..26880
                     /gene="rhaM"
                     /locus_tag="ACHJZ2_002243"
     CDS             26566..26880
                     /gene="rhaM"
                     /locus_tag="ACHJZ2_002243"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO4568339.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(26921..28315)
                     /locus_tag="ACHJZ2_002244"
     CDS             complement(26921..28315)
                     /locus_tag="ACHJZ2_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO4568340.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            28793..29239
                     /gene="frvA"
                     /locus_tag="ACHJZ2_002245"
     CDS             28793..29239
                     /gene="frvA"
                     /locus_tag="ACHJZ2_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO4568341.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            29250..30689
                     /gene="frvB"
                     /locus_tag="ACHJZ2_002246"
     CDS             29250..30689
                     /gene="frvB"
                     /locus_tag="ACHJZ2_002246"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO4568342.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            30679..31749
                     /gene="frvX"
                     /locus_tag="ACHJZ2_002247"
     CDS             30679..31749
                     /gene="frvX"
                     /locus_tag="ACHJZ2_002247"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4568343.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            31749..33497
                     /gene="frvR"
                     /locus_tag="ACHJZ2_002248"
     CDS             31749..33497
                     /gene="frvR"
                     /locus_tag="ACHJZ2_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO4568344.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(33547..>34601)
                     /gene="yiiG"
                     /locus_tag="ACHJZ2_002249"
     CDS             complement(33547..>34601)
                     /gene="yiiG"
                     /locus_tag="ACHJZ2_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO4568345.1"
                     /translation="KRNLLSSAIIVAIMALGLTGCDDKKAETETPLPANSQPAAPAPE
                     AKPTEAPVAKADAKPETPAQPLVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWL
                     KDFKQGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVA
                     LGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQ
                     LAELKNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAK
                     EADKGGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPR
                     ALREYNEMVDDYNSLR"
BASE COUNT         8546 a         8834 c         8628 g         8593 t
ORIGIN      
        1 gccgttaacc atttccgcta caaatttctt ttatctggca acgcgggtga aatgatttta
       61 tattttgcta acgtattttt taacccactt gccttatcat ttattatcgc cacaatcatc
      121 tgtataacga taaaaaaaag aagcagccgc agctttattc gcggcacttg ttggttaatg
      181 ggattatttc ttatctatag cagctatacg gtgtgggaac gacacagcat atgggattat
      241 actttcccgg agccaggtat caccgtcacg gttccgagca aacagtgggt tgccaatata
      301 gtaaaacaag gaccaaccct ggtgacccgg gatgcccatg ctattttagc tattgttgcc
      361 ttctcacaaa atgaactcgg tgtgcattca atcgaggaac ttaccgcgac tcaaaacgga
      421 actgcagaaa tgcatgtttg tgatgttcag ggatttgcct gcgcttatca ggaaggagtg
      481 caaacaatga gtgatggcaa agtaagacat gctattttca tgactctact ggataacact
      541 aatttgatac aactggtcgc agctatcgac ccagattatt tggacgaata tcaagaagaa
      601 gtaatgaaaa tgatgctttc tgctcgtcaa tgatctgatg tgcatgttaa taataatgga
      661 ttgatatgag atacgttatc ttattaatac tatggataat aactgttttt cttagtcatc
      721 ttcagcacag taacttgctt actggagatc cagggatggt tggcgagtat atcggttcag
      781 tacttcttgg accgttatta cttcccgtgc ttgtgtcagg tattctgtgc acattcacta
      841 aaaaaacgcg taacttcgcc agttttactc gcgggtgctg ttgggtattg ggagtcctgc
      901 ttttatccaa cgtcggcaat acgtttcgtc tgttcacccc gtggcactat acgtttgaga
      961 aggcagctat ttccgtgact gtcccaaacc gacactggaa tacggtttct attagtactg
     1021 ataaaaccat tgatataaga agtgaagata atagcgtctt tatttctgct ttccgcctgc
     1081 ccgcaggacg tagcgccgat gactcgctgg aagagttaaa gaagatgcag cgagacaatc
     1141 tcaaggatca atacaatgaa gaaaccttcc agtttcatga ttgtaatgcc aaacatttca
     1201 cctgtaagta tcaggatgtg ttgataaatt ttgatggtca gcagaaaaga accatcagtg
     1261 tttatcttga agacaccccc agggctgtgg ggatcattgc attaatggag ccagatacgg
     1321 cagataaata tcgccaacag gcgatggaga ttatgttgtc tgctaaaaat acagtgaagt
     1381 aaaaaataag gcgccttgcg gcgcctctgc gtggaagaga ttaaacctct tccatgcgac
     1441 ccagcagggc ctgcagacgt tcctgccagc cgttctgctg ttctttcaga tggttgttct
     1501 cacgctccag ctcttcgcgc tgatgctggg cattttgaac ttcctgcgac aatgagttgt
     1561 ttttttcttt cagctcttcg atttccatct gcaacagagt gatggtatca atcgcctgct
     1621 gtacttttgc ttccagtttc tcaaacactt ctaatgacat tgtcatacct ctcctgaatt
     1681 gcaaggcgtt gatggataaa aaatcctcgt cccgattacc ggtgacgcct taataaatac
     1741 gagcgcactt tagttagctc cgattgtatg aagccgcgcc atcgctgtcc agcggcacgc
     1801 cttgcagatt acggtttgcc acacttttca tccttctcct ggtgacataa tccacaccaa
     1861 tcgaaaatgt taataaattt gttgcgcgaa tgttctaaca aacatgcatc atgtacaatc
     1921 agatggaata aatggcgcga taacgctcat tttatgacga agcacacaca ttttaagttc
     1981 gatatttctc gtttttgctc gttaacgata agtttacagc atgcctacaa gcatcgtgga
     2041 ggtccgtgac tttcacgcat acaacaaaca ttaactcttc aggatccgat tatgagtcaa
     2101 acatcaacct tgaaaggcca gtgcattgct gaattcctcg gtaccgggtt gttgattttt
     2161 ttcggtgtgg gttgcgttgc agcactaaaa gtcgctggtg cgtcttttgg tcagtgggaa
     2221 atcagtgtca tttggggact gggggtggca atggccatct acctgaccgc aggggtttcc
     2281 ggcgcgcatc ttaatcccgc tgttaccatt gcattgtggc tgtttgcctg tttcgacaag
     2341 cgcaaagtta ttccttttat cgtttcacaa gttgccggcg ctttctgtgc tgcggcttta
     2401 gtttacgggc tttactacaa tttatttttc gacttcgagc agactcatca cattgttcgc
     2461 ggcagcgttg aaagtgttga tctggcaggc actttctcta cttaccctaa tcctcatatc
     2521 aattttgtgc aggctttcgc agttgagatg gtgattaccg ctattctgat ggggctgatc
     2581 ctggcgttaa cggacgatgg caacggtgta ccacgcggcc ctttggctcc cttgctgatt
     2641 ggtctactga ttgcggtcat tggcgcatct atgggcccat tgacgggttt tgccatgaac
     2701 ccagcgcgtg acttcggtcc gaaagtcttt gcctggctgg cgggctgggg caatgtcgcc
     2761 tttaccggcg gcagagacat tccttacttc ctggtgccgc tttttggccc tatcgttggc
     2821 gcgattgtag gtgcatttgc ctaccgcaaa ctgattggtc gccatttgcc ttgcgatatc
     2881 tgtgttgtgg aagaaaagga gaccacaact ccttcagaac aaaaagcttc gctgtaatat
     2941 gactacggga caattaaaca tgactgaaaa aaaatatatc gttgcgctcg accagggcac
     3001 caccagctcc cgcgcggtcg taatggatca cgatgccaat atcattagcg tgtcgcagcg
     3061 cgaatttgag caaatctacc caaaaccagg ctgggtagaa cacgacccaa tggaaatctg
     3121 ggccacccaa agctccacgc tggtagaagt gctggcgaaa gccgatatca gttccgatca
     3181 aattgcagct atcggtatta cgaaccagcg tgaaaccact attgtctggg aaaaagaaac
     3241 cggcaagcct atctataacg ccattgtctg gcagtgccgt cgtaccgcag aaatctgcga
     3301 gcatttaaaa cgtgacggtt tagaagatta tatccgcagc aataccggtc tggtgattga
     3361 cccgtacttt tctggcacca aagtgaagtg gatcctcgac catgtggaag gctctcgcga
     3421 gcgtgcgcgt cgtggtgaat tgctgtttgg tacggttgat acgtggctta tctggaaaat
     3481 gactcagggc cgtgtccatg tgaccgatta caccaacgcc tctcgtacca tgttgttcaa
     3541 catccatacc ctggactggg acgacaaaat gctggaagtg ctggatattc cgcgcgagat
     3601 gctgccagaa gtgcgtcgtt cttccgaagt gtacggtcag actaacattg gcggcaaagg
     3661 cggcacgcgt attccaatct ccgggatcgc cggtgaccag caggccgcgc tgtttggtca
     3721 gttgtgcgtg aaagaaggga tggcgaagaa cacctatggc actggctgct ttatgctgat
     3781 gaacactggc gagaaagcgg tgaaatcaga aaacggccta ctgaccacca tcgcctgcgg
     3841 cccgactggc gaagtgaact atgcgttgga aggtgcggtg tttatggcag gcgcatccat
     3901 tcagtggctg cgcgatgaga tgaagttgat taacgacgcc tacgattccg aatatttcgc
     3961 caccaaagtg caaaacacca atggtgtgta tgtggttccg gcatttaccg ggctgggtgc
     4021 gccgtactgg gacccgtatg cgcgcggggc gattttcggt ctgactcgtg gggtgaacgc
     4081 taaccacatt atacgcgcga cgctggagtc tattgcttat cagacgcgtg acgtgctgga
     4141 agcgatgcag gccgactctg gtatccgtct gcacgccctg cgcgtggatg gtggcgcagt
     4201 agcaaacaat ttcctgatgc agttccagtc tgatattctc ggcacccgcg ttgagcgccc
     4261 ggaagttcgc gaagtcaccg cattgggtgc ggcctatctc gcaggcctgg cggttggctt
     4321 ctggcagaac ctcgacgagc tgcaagagaa agcggtgatt gagcgcgagt tccgtccagg
     4381 catcgaaacc actgagcgta attaccgtta cgcaggctgg aaaaaagcgg tcaaacgcgc
     4441 gatggcgtgg gaagaacacg acgagtaatg tcaggcacct gaatcgattg cctgatgctg
     4501 atcgcctgat gcgacgctta cgcgtcttat caggcctacg agccaatgcc attttgtgta
     4561 ggcgggataa ggcgttcacg ccgcatccgg catcacgcag actctgaaac cgtgcggggg
     4621 caaccccgca cacatcaata atcccaccct tcccctgtgc tacacttcgc gccattcctt
     4681 actgcttaga gtttgctatg agacgagaac ttgccatcga attttcccgc gtcaccgaat
     4741 ctgcggcgct ggctggctac aaatggttag gacgcggcga taaaaacacc gcggacggcg
     4801 cggcggtaaa cgccatgcgt attatgctca accaggtcaa cattgacggc accatcgtca
     4861 ttggtgaagg tgaaatcgac gaagcaccga tgctctacat tggtgaaaaa gtcggtactg
     4921 gtcgcggcga cgcggtagat attgctgttg atccgattga aggcacgcgc atgacggcga
     4981 tgggtcaggc taactcgctg gcggtgctgg cagtaggcga taaaggctgt ttcctcaacg
     5041 cgccagatat gtatatggag aagctgatcg tcggaccggg agccaaaggc accattgatc
     5101 tgaacctgcc gctggcggat aacctgcgca atgtcgcggc ggcgctcggc aaaccgttga
     5161 gcgaactgac ggtaacgatt ctggctaaac cacgccacga tgccgttatc gctgaaatgc
     5221 agcaactcgg cgtacgcgta tttgctattc cggatggcga cgttgcggcc tcaattctca
     5281 cctgtatgcc agacagcgaa gttgacgtgc tgtacggaat tggtggcgcg ccggaaggcg
     5341 tggtttctgc ggcggtgatc cgcgcattag atggcgacat gaacggtcgt ctgctggcgc
     5401 gtcatgacgt caaaggcgac aacgaagaga atcgtcgcat tggcgagcag gagctggcac
     5461 gctgcaaagc gatgggcatc gaatccggta aagtattgcg tctgggcgat atggcgcgca
     5521 gcgataacgt catcttctct gccaccggta ttaccaaagg cgatctgctg gaaggcatta
     5581 gccgcaaagg caatatcgcg actaccgaaa cgctgctgat ccgcggcaaa tcacgcacca
     5641 ttcgccgcat tcagtctatc cactatctgg atcgcaaaga cccggaaatg caggtgcaca
     5701 tcctctgatt gatttgatcg attgagcctt ccagtccttc gggactggaa tttttttgtt
     5761 cggagaacga agataaggca attcaatcaa aacaggagaa aaacatggct gattgggtaa
     5821 caggcaaagt cactaaagtg cagaactgga ccgacgccct gtttagtctc accgttcacg
     5881 cccctgtgca tccgtttacc gccgggcaat ttaccaagct tggccttgaa atcgacggcg
     5941 aacgcgtcca acgcgcctac tcctatgtta actcgcccga taatcccgat ctggagtttt
     6001 acctggtcac cgtccccgat ggcaaattaa gcccacgatt ggcggcactg aaaccgggcg
     6061 atgaagtgca ggtggttagc gaagcagctg gcttctttgt tctggatgaa gtaccagatt
     6121 gcgaaacgct atggatgctg gcaaccggta cagcgattgg cccttattta tcgattctgc
     6181 aactaggtaa agatttagat cgcttcaaaa atctggtcct ggttcacgcc gcacgttatg
     6241 ccgccgactt aagctatttg ccactgatgc aagaactgga aaaacgctac gaaggcaaac
     6301 tgcgcattca gacggtggtc agtcgggaaa cggcagcggg gtcgctcacc ggacggatac
     6361 cggcattaat tgaaagtggt gaactggaaa gcgcgattgg cctgccgatg aataaagaaa
     6421 ccagccatgt gatgctgtgc ggcaatccac agatggtgcg cgatacgcaa cagttgctga
     6481 aagagacccg gcagatgacg aaacatttac gtcgccgacc gggtcacatg acagcggagc
     6541 attactggta agcggttact tatcgataaa cggcacgatg agcaaatccg cactcaactt
     6601 attgatcatc ccgcgatatg ccggcatcaa acggttgata aatgagtggt gatgaccaca
     6661 gacaaggagg tcgcactgct ctttttgcat aatttccagc agtgtttccg gcatttctcc
     6721 gcgttcaata cgcagttttg tcttcggcca ttgaatattt ttcgtcagtt tatacagctt
     6781 gttatccgac ttattcttca acaattgaag aatatcttct gttgcaggga agtagatacc
     6841 cggatacaac tcgcttaagc catcatcaat atgaattaac gtcaggtgag cgtcattatg
     6901 tctggcgagc tccaaggctt tattcaccag taaggcatct tcttcattcc cggaaattgc
     6961 cacgccaatg tgtttataag ccatgtttaa ctccttctaa agcaactcca tcaagctaac
     7021 gaacgcagtg ataactcaaa aataatcatc tcggcctggc atgagaaggt gaaggccgca
     7081 tcaagctcaa cttgcccctc cagatcctta atcttatagg ccacgctggc cccttcatct
     7141 gcagaggatg cagcggcggc aagctctttc agtttggtca taaaggactc tgcttcttcg
     7201 cgggtggcaa aaaagcgcga aaacttactg gtacagttgt cgttatcaat caccgtaccg
     7261 atatcaatcg cacatccttt agtactgcat ttatctacga catctttcat agggcacctc
     7321 tgtatattcg cctctctgtc gattacgact taagaataat gcgccgttaa gggaagaaca
     7381 aacaagtaat gatcttatgg attaccattt tttttgatat tgcgtaaggt gaagagttca
     7441 gaaggatttt ccgggtattg tcatttgcat cgtggaaata acaatcccgg aaaaaaatag
     7501 cagtaagaga tatattaaag gttaattact tgatttattg tcggctttat acttcacatc
     7561 ctgagtatct ttaccatatt tattttcgcc ttgtgtgcca acaaacgcac caagatcgat
     7621 aagcatcatc accagaatca acgtcgggac aaaacgccct accgcccatt gccagacacc
     7681 cggtaaaatc gcccagttac ccgccagtag catccacgcc acaatcatca gaaatgccca
     7741 tgcgccggaa cgcccgcgat catgcaagcg cttaacggtt actgccgctg ttggccagag
     7801 caagcacaca aggcaaaacg ccgcggtctg aatatcgagt aaatttttac ccgccagtga
     7861 gaaaagcacc agcatgcctg cgaaccacag gcctatccaa atccagaaat cacggcgtcc
     7921 aatacgccct ttaaatgaga ataaccattg ctgtatggtc atgtaagttc cttgatggtt
     7981 gtcttttcca ggattctacc cttttgacaa gggcgacaga tatcgtttta atcggagcca
     8041 gtcataacaa aaggtactgt caatgaagcc agggtgtacg ctgttttttc tcttatgttc
     8101 tgcattaacc gttacaacaa cggcgcatgc acaaacacca gatacggcaa cgaccgcgcc
     8161 ttatctgctg gctggagccc ctactttcga tctctccatc agccagtttc gagaagactt
     8221 taacagccag aatcccagcc tgccactgaa cgaatttcgt gccatcgaca gcagtcccga
     8281 caaagccaat ctcactcgtg ctgccagtaa aattaacgag aacttgtatg cttctacagc
     8341 gctggagcgc ggtaccttaa aaatcaaaag cattcaaatg acatggctac ccatccaggg
     8401 gccagagcaa aaagccgcga aagcgaaagc tcaggaatac atggcagcgg tgatccgcac
     8461 actcacccca ttaatgacca aaacacaaag ccagaaaaaa ctgcagtcgc tactaacggc
     8521 ggggaaaaac aaacgttatt acaccgagac agaaggtgca ctgcgctatg ttgtcgcgga
     8581 caacggcgaa aaggggctga ccttcgctgt tgaaccgatt aagctggcgc tatctgaatc
     8641 gcttgaaggt ttgaataaat gacaaaaagc aaagcctttg tgccgatgaa tctctatact
     8701 gtttcacaga cctgctgccc tgcggggcgg ccatcttcct ttattcgctt ataagcgtgg
     8761 agaattaaaa tgcgacatcc tttagtgatg ggtaactgga aactgaacgg cagccgccac
     8821 atggttcacg agctggtttc taacctgcgt aaagagctgg caggtgttgc tggctgtgcg
     8881 gttgcaatcg caccaccgga aatgtacatc gatatggcga agcgtgaagc tgaaggcagc
     8941 cacatcatgc tgggtgcgca aaacgtggac ctgaacctgt ccggcgcatt caccggtgaa
     9001 acctctgctg ctatgctgaa agacatcggc gcacagtaca tcatcatcgg tcactctgaa
     9061 cgtcgtactt accacaaaga atctgacgaa ctgatcgcga aaaaattcgc ggtgctgaaa
     9121 gagcagggcc tgactccggt tctgtgcatc ggtgaaaccg aagctgaaaa cgaagcgggc
     9181 aaaactgaag aagtttgcgc acgtcagatc gacgcggtac tgaaaactca gggtgctgcg
     9241 gcattcgaag gtgcggttat cgcttacgaa cctgtatggg caatcggtac tggcaaatct
     9301 gcaactccgg ctcaggcaca ggctgttcac aaattcattc gtgaccacat cgctaaagtt
     9361 gacgctaaca tcgctgaaca agtgatcatt cagtacggcg gctctgtaaa cgcgtctaac
     9421 gctgcagaac tgtttgctca gccagacatc gacggcgcgc tggttggcgg tgcgtctctg
     9481 aaagctgacg ctttcgcagt aatcgttaaa gctgcagaag cggctaaaca ggcttaagtc
     9541 tgacaggtgc cggatttcat atccggcact tactttcctt aactcttcgc cttaacgcaa
     9601 aatctcacac tgatgatcct gaatttcctc ggctgaagca cggttaagcg tcagtagatt
     9661 tcgttgtgtc gccagcaata caaatgagtt atcactctgc cgtaccatcg ccagcccgta
     9721 gcttcccata tgttcccgcg cctcaggtac ttcttctgcc agcatcataa atgggctgcg
     9781 ttgtaccagt tcgctttccg ttacccgacg cgccaggtat tcatgcccgc gcaaaccacc
     9841 tggcagtggc aaccagcggc tgctgatgtt cgccagattg ttatccagct gttcgcgcac
     9901 atcaggacga atacaagaga tatgaatatg aaaatggttt tgcgtacgcc cggtgcggga
     9961 gttgatcgcc aaagaaaccg cgcgatcggg aaccggctgg ccgtattttt tgctcataaa
    10021 atcacgcgcc tgccaggcca accaaaagaa gttcggcgtt gaaggatcgg tcaacaaagg
    10081 actttcagta ccgttaatac gatacgttgg catcaacaga tattgcagtg ggccattaag
    10141 atcttttaaa accacgtatc cggcattggg tttgacttcc gcacatggcg aaggattttg
    10201 attttgctgc tgattgggca aacattcctc aaggacaatc ttacgtaatg tatccgactc
    10261 ttcaccggtt aatttccagt aaccaatacc ggcagccaca acggcgataa ctatcatcac
    10321 caaaaaaaga agacccgctt ttttcatctt tttttccctg tacctcaaag agatgcaagg
    10381 gtaacgcaaa atcgtgacaa ataaaaaacc cggtcgcgag aaaccaccgg gtttgataat
    10441 tatcctgggg aaatcagcgt ttgctgatct gatcgaacgt accgccgtta gcaaaatgct
    10501 ctttttgcgc tttcgtccag ccgccgaact cttcatcaat ggtgaataac ttcagctttg
    10561 gaaacgcatt ttcgtacttt ttcgccacct cagcgtcgcg cggacggtag tagtttttcg
    10621 cggcaatttc ctgaccttct ggcgagtaga gatatttcag gtaggcttcc gccacctctt
    10681 tagtaccttt tttctcgacc actttatcga ccaccgacac ggttggctct gcgaggatag
    10741 actcactcgg cgtgacgatt tcgaatttat ctttccccag ttcattcgct gccagcagag
    10801 cttcgttttc ccaggaaatc agtacatcgc caattccgcg ctcgacaaaa gtgttagtgg
    10861 agccgcgcgc gccagaatcc agaacttcga cgtttttata cagtgcccga acaaaatcct
    10921 gtgcttttgc ctgatcgttg ttgttgtgat gcagcgcgta gccccaggct gccaggtagt
    10981 tccagcgcgc gccaccagag cttttcggat taggcgtgat caccgaaaca cccagtttaa
    11041 tcagatcgtt ccagtcatgg atctgcttcg gatttccctt acgtaccagg aaaacaatgg
    11101 tggaagtgta cggtgcggag ttatccggca gacgtttgat ccactcttta tcaatccgcc
    11161 cgcgttccgc aatcgcgtcc acgtcatagg ccagagccag cgtgacaaca tcagcttcaa
    11221 taccgttgat taccgacgtt gcttgtttgc ctgaaccacc gtgtgactga cgaatcacca
    11281 cgttatcgcc agtttgctgt ttccagtggg cgctgaatgc cttgttgtac tgttcgtaca
    11341 attcgcgcgt tggatcatat gaaacgttaa gaagctgaat atcctttgcc ataacgctgg
    11401 ttgccgccag caaaaatgtt aaccctacgc cccacttgtt catcgcccga ctctcttatg
    11461 ttgtgttgtg atgagcaaag cgtgccagaa ggttaaccaa acattaaaga ataaaaaaag
    11521 attggctata acttgcgggt atatgttggg ggttaaaaag gcggaagaaa tccgcctcat
    11581 attgctgaca aagtgcgctt tatcgatgcc ggatgcggcg cgaacgcctt atccggccta
    11641 caaaagtttg taaattcaat aaattgtaga attcacgtag gcctgataag cgaagcgcat
    11701 caggcatttt tgcttctgtc atcggtttca ggctaaagga atctgccttt ttccgaaatc
    11761 attagtacag ttttttcgcg cagtccagcc agtcgccttt gaacggacgc ttcatgtttt
    11821 cgatagcgtc gatgatgtcg tggtgaacca gctgttcgtt ctgtatacct acgcaacgac
    11881 cgccgtaacc tgccagcagc agatcgatag cgtaagcgcc catacgggaa gccagaatac
    11941 ggtcgtaagg caccggagaa ccaccgcgct ggatgtggcc cagcacagtt gcgcgggttt
    12001 cacgaccggt ttctttctcg atgaaatgcg ccagttcgtc aacatcacac atatgttcgg
    12061 taatcgccac gatcgcgtgt tttttacctt tcgcgatacc cgctttgatt tcgtttacca
    12121 ggtcttcacg gctgaattca acttccggaa ccacaacgaa ttcacagcca ccggcaatgg
    12181 ccgcagccag cgtcagatcg ccacaataac ggcccatcac ttccaccacg gaaatacgct
    12241 ggtgagaaga agaggtgtca cgcagacggt cgatcgcttc tacaacggtg ctcagcgcag
    12301 tgaagaaacc gatagtgtag tcagtgcctt tgatgtcgtt gtcgatagtg cccggcaggc
    12361 cgatgcacgg gaagcccatt tcggtcagac gcattgcacc catgtaggaa ccgtcaccgc
    12421 cgataaccac cagcgcatcg atcccacgtt ttttcaggtt ttcgatagcc acggcgcgga
    12481 tgttctcgtc gcggaattcc gggaaacgcg cagaaccgag gaacgtaccg ccacggttga
    12541 tcatgtcaga aacgctgtaa cggtctagct gtaccatacg gtcttcatac agacccagat
    12601 agccgtcata aatgcccatt acttccagac cttctgtcag cgcagaacga acaaccccgc
    12661 gaattgcggc gttcatgcct ggcgcatcac cgccgcttgt caacacaccg attttcttaa
    12721 tcatgactac ctctgaactt tggaatgcaa aataaaatct gttgccggaa gtcttcttgc
    12781 acatcgaagt gatccaacga atgtgcaaat agtataacaa tcacttcctg ctgaattgat
    12841 tcaggtcagg ccaaatggcg gtattttata cacaaaatgc gggtctggct ctcttttata
    12901 ctgattatga aagcatagac cgtttaccct ccctaggtac gacggaacag gggtcctgat
    12961 ggataatcac atccgatccc ggaaaacgcc gtaaaatagc ctgctctacc tgatccgcca
    13021 ccatatgtgc ctgaaccaaa ggcagagagt cttccatttc caaatgaatc tgaataaagc
    13081 gggtcggccc tgactgccgc gtgcgaagat cgtgagcgcc gctaacaccc ggccaggaag
    13141 tcacgatatc aataatttct tgccgttcct catcaggtaa tgcgcgatcc agtaatgact
    13201 gtaccgcctc atatcccatg cgtaacgcgc tatataaaat atagatgccg attcccaatg
    13261 caaacagagc atcggcgcga tgccagccgt accaggacaa ccccagcgcc agcagaattg
    13321 cgccgttcat cataacatca gactggtaat gtagcatatc agcccgcacc gcctggcttt
    13381 gcgtccggcg caccacccaa cgctgaaacg agacaaggat aatcgtacaa attagcgcca
    13441 caattgtcac gataaccccg acgcctggat ctgtcatcgg tgttggagat atcagatgtt
    13501 gaatgcctgt caaaaacagg aatagtgccg aaccggagat aaacatactt tgcgccagcg
    13561 ccgcgaggga ctcagcttta ccgtgaccaa acgagtgatt atcgtcggca ggttgcaggg
    13621 aatatcgcac caccaataaa ttcgtcaacg acgcgccgat atccaccagc gaatccacca
    13681 gcgcggcgag aatactcacc gacccggtat accaccatgc aaaaatttta atcagcaata
    13741 gcagtgaagc catcgccgtc gcagcaatcg ccgcccgact gaccagccgt ccataagatt
    13801 gattcataaa tactcccgct atcaactgac gctagtataa cggaagcaaa tcatctgcaa
    13861 tgcattaagc agcaggcaaa ttgaggataa aaaaaacccc cacatcatgt gggggaagac
    13921 agggatggtg tctatggcaa ggaaaacagg gtttactact gggaacgtga gttgctacta
    13981 ctcaatagct tcaacgatga acttttttgc cattgcgtca cgtcacgcaa ctgctccatt
    14041 cgttgttgat gtttctcgtt taaaaccgct tgctgctccg gcgttaacag gcgatacatt
    14101 tggttgcgga cttttgccat ctcaacctga cgagcaattt gctcattcgc cattttttct
    14161 gcctgtgcgc gcacagcgtt ttcatcaaaa ttttctgcgg tgacaagacg atgcattgtc
    14221 tccagttcgc taacattaac aggaggctgt tcgtgccggg cctgttgcat aagatctcgc
    14281 atctgctgac gctgatgttc ggttaaactt atgccgtcga acatatggct ctgcgtactg
    14341 cgctgcgtaa gttcttcacc cggatgccag ttatcgcctg aaccgacttc agcagcgtgg
    14401 cttaatgaac tgactgccag cgttgaggcc atgacggcag cggtaactat gcgcatcatt
    14461 tgctcccaaa atctttctgt cgcgattcaa cgatagagag tttacgattc aggctgcaaa
    14521 catgcgtcag ggggtgtaaa acaacgtaaa gtcatggatt agcgacgtct gatgacgtaa
    14581 tttctgcctc ggaggtattt aaacaatgaa taaaatcctg ttagttgatg atgaccgaga
    14641 gctgacttcc ctattaaagg agctgctcga gatggaaggc ttcaacgtga ttgttgccca
    14701 cgatggggaa caggcgcttg atcttctgga cgacagcatt gatttacttt tgcttgacgt
    14761 aatgatgccg aagaaaaatg gtatcgacac attaaaagca cttcgccaga cacaccagac
    14821 gcctgtcatt atgttgacgg cgcgcggcag tgaacttgat cgcgttctcg gccttgagct
    14881 gggcgcagat gactatctcc cgaaaccgtt taatgatcgt gagctggtgg cacgtattcg
    14941 cgcgatcctg cgccgttcgc actggagcga gcaacagcaa aacaacgaca acggttcacc
    15001 gacactggaa gttgatgcct tagtactgaa tccaggccgt caggaagcca gcttcgacgg
    15061 gcaaacgctg gagttaaccg gtactgagtt taccctgctc tatttgctgg cacagcatct
    15121 gggtcaggtg gtttcccgtg aacatttaag ccaggaagtg ctgggcaaac gcctgacgcc
    15181 tttcgaccgc gctatcgata tgcacatttc caacctgcgt cgtaaactgc cggatcgtaa
    15241 agatggtcac ccgtggttta aaaccttgcg tggtcgcggc tacctgatgg tttctgcttc
    15301 atgataggca gcttaaccgc gcgcatcttc gccatcttct ggctgacgct ggcgctggtg
    15361 ttgatgttgg ttttgatgtt acccaagctc gattcacgcc agatgaccga gcttctggat
    15421 agcgaacagc gtcagggtct gatgattgag cagcatgttg aagcggagct ggcgaacgat
    15481 ccgcccaacg atttaatgtg gtggcggcgt ctgttccggg cgattgataa gtgggcaccg
    15541 ccaggacagc gtttgttatt ggtgaccacc gaaggccgcg tgatcggcgc tgaacgcagc
    15601 gaaatgcaga tcattcgtaa ctttattggt caggccgata acgccgatca tccgcagaag
    15661 aaaaagtatg gccgcgtgga actggtcggt ccgttctccg tgcgtgatgg cgaagataat
    15721 taccaacttt atctgattcg tccggccagc agttctcaat ccgatttcat taacttactg
    15781 tttgaccgcc cgctattact gctgattgtc accatgttgg tcagtacgcc gctgctgttg
    15841 tggttggcct ggagtctggc aaaaccggcg cgtaagctga aaaacgctgc cgatgaagtt
    15901 gcccagggaa acttacgcca gcacccggaa ctggaagcgg ggccacagga attccttgcc
    15961 gcaggtgcca gttttaacca gatggtcacc gcgctggagc gcatgatgac ctctcagcag
    16021 cgtctgcttt ctgatatctc tcacgagctg cgcaccccgc tgacgcgtct gcaactgggt
    16081 acggcgttac tgcgccgtcg tagtggtgaa agcaaggaac tggagcgtat tgaaaccgaa
    16141 gcgcaacgtc tggacagcat gattaacgac ctgttggtga tgtcacgtaa tcagcaaaaa
    16201 aacgcgctgg ttagcgagac catcaaagcc aatcagttgt ggagtgaagt gctggataac
    16261 gcggcgttcg aagccgagca aatgggcaag tcgttgacag ttaacttccc gcctgggccg
    16321 tggccgctgt acggcaaccc gaacgccctg gagagtgcgc tggaaaacat tgttcgtaat
    16381 gccctgcgtt attcccatac gaagattgaa gtgggctttg cggtagataa agacggtatc
    16441 accattacgg tggacgacga tggtcctggc gttagcccgg aagatcgcga acagattttc
    16501 cgtccgttct atcggaccga tgaagcgcgc gatcgtgaat ctggcggtac aggtttggga
    16561 ctggcgattg ttgaaaccgc cattcagcag catcgtggct gggtgaaagc agaagacagc
    16621 ccgctgggcg gtttacggct ggtgatttgg ttgccgctgt ataagcggag ttaaactccg
    16681 catttgtagg caggataagg cgtttacgcc gcatccggca tttgagcagg atgcctgatg
    16741 cgacgctgat agcgtcttat caggcctaca ctccgggggt tattttcccc acaaccggcg
    16801 ggaaaaatct tcgatcttgc cgctcagtcg gcgcttttgc atcgttcgcg tccagctttt
    16861 cgataacccg gcagcggaga gtaaacggtg atactgatcg tcatcaaacg gcatatgcca
    16921 tgcgatggca gcagcctcct gcacggtcac atcgctgaca cgggaaacca actccagcgg
    16981 cgcgtccgca gacactttcc ctggcgctat cacgctatac aaccatccca ctttacccgt
    17041 gttttgcatc aactgcgcta tatcgctgat atcaaaatgg taattgagtt tatagcaagg
    17101 cgagcgcggc tggctgacct gaattaatgc ctctccccag cggaaaatat cgccaatata
    17161 aacattactt tccgtgagac cgtcggttga gagattttcg ccaaacgcag gcgcaacaaa
    17221 caactccgcc tgttccggaa attcccgcgc ccagtagaga taatgctcac gaggataatg
    17281 acacagcgct ctgtctggtc cgccgtgaac ctttttctcc gcctgctcgt caccttccag
    17341 ccccagctcc gttagcatca actcaccatc aacctggatt ttggcaattg cgctgggttt
    17401 gccttcggga taagcctgaa tcttgcctgt gtatacatca accggatatc gcatctgttc
    17461 cctctccacg agaaatttcc ccattagacc atatttattt tatgtcaggc gtgctgtttt
    17521 cgcggtaagt atccaggcag gaactcaata gcaaaccgga cgaacgttag ttcatccggc
    17581 tgtggggatt aaggaagcag gtttatttca ccgtacctaa aatttcgatt ttcgccgctc
    17641 tggctttgac tttacgtgac cagatagagg taagaattgg caccagaatc gaagtcacaa
    17701 tgactgccgt cgctaccagc gaagttgctg ccggagccat cggtttaaac gcaggcacca
    17761 tttctgcaat cagcacaggt gtcgctaccg cggcccctgc ggaactggaa gcggcaattc
    17821 cggcagtgcc atcgccaccg ccaatcaatt tatcggcgat aatcaacgga ataccggtca
    17881 cgataattac tgccacaccc aacaggatcc ccagcaaacc tgtctgggca attacagtca
    17941 aatcaatggt attgcctagt gcgaaggcaa agaatggaat cagcgtctgc accgctttgc
    18001 tgaaaaattc tcgtaattca gggtcaaggt tcccaagggc aaagcccacc aggaacggta
    18061 atactgcgcc gacgaaaaca tgcggttcaa acgaggcaat cccggcagtg cccagaataa
    18121 tcatcgtcat gagcggcccg gactccaacg acatcaacac aaatgcccca gcttcttctt
    18181 ttgtgccgta ctgctgcatg atggaagcgt aaagtccgcc gttggtcata tccatcgccg
    18241 ccaccagcgc cagcgttgaa agtccggcaa agaatccaac ttcaacacca tgttccggaa
    18301 taatgcgcga ggcaattgcc gcaaccaccc acgcgacggc aattttagtt accaccagcg
    18361 taccggattt acgcagtacc gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa
    18421 aaaaccacac cgccagaatg ggcaccgtac cggtaatcat cccgttggtg aatgatccaa
    18481 aatatttccc cgcccccggc gagaaggtgt ggcacagtgc gccaaggaat agcgggacga
    18541 gcatcatccc ccccgggatt ttctcaatca agcgttttat ctgcatttcc atcacctgtc
    18601 ggcatatttt taagggctga tgcgtaattc ctcaatggag catcagtgtt gatggaaacg
    18661 aagatagaca ttcatttaat caataaaagt gatcaaaacc acatcaactg aaacgctgtt
    18721 ttatttttat cggatcattg tttttatttt gaaattgatc acaaaaaaac caccgccatt
    18781 ggcgatggtt cattttttaa gctgatatgc ggcgtaaacg cctcactgca gcagacggca
    18841 aatgattatt ttttcgccgc gaaacgtgcc gctgcttcgt cccagttcac cacgttccag
    18901 aactctttga tgtagtccgg gcggcggttc tggaatttca ggtagtaagc atgctcccac
    18961 acatccaggc ccagaatcgg gaagccggat gcgccagaaa tagcttcacc catcagcggg
    19021 gagtcctggt tagcagtaga aaccaccgcc agtttatcgc ctttcagcac cagccatgcc
    19081 cagccggaac caaagcggga agctgccgct ttctcaaatt ctgctttgaa gttatctacg
    19141 gaaccgaaat cacgttcgat agccgctttc aggtcaccct gcagggtggt gccttttttc
    19201 agacctttcc agaacaggct gtggttagcg tgaccgccag cgttgttgcg cagtacggtt
    19261 ttcttgtctg ctggcagctg gtccagtttg gtgatcaact cttcaaccgg caggttggca
    19321 aattctggca ggctttccaa cgccgcgttg gcgttgttga cgtaggtctg atggtgtttg
    19381 gtgtggtgga tttccatggt ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac
    19441 ggcagggatg gcagggtata gctcatattc atctccagta ttgtcgggcg gccgattgtt
    19501 aatgccgcgt aagcagttgg ttcattatag ttaattaaat gatattgaaa atgattatca
    19561 atgccgtact tttcgtaagg gtatggtttt gcaggaaaat gcccgagatg tgaagcaaat
    19621 cacccactta atgccgtgat tgccagtaaa tcgacaacgg cggcaacagg cgaaaggtta
    19681 atcgacagca cgatttttac actcatctcg tcggagatgt gacgcgacga aaaatgatga
    19741 ggataagaag atgagtaacg cgattacgat ggggatattt tggcatttga tcggcgcggc
    19801 cagtgcagcc tgtttttacg ctccgttcaa aaaagtaaaa aaatggtcat gggaaaccat
    19861 gtggtcagtc ggtgggattg tttcgtggat tattctgccg taggccatca gcgccctgtt
    19921 actaccgaat ttctgggcgt attacagctc gtttagtctc tctacgctgc tgcctgtttt
    19981 tctgttcggc gctatgtggg ggatcggtaa tatcaactac ggcctgacca tgcgttatct
    20041 cggcatgtcg atgggaattg gcatcgccat tggcattacg ttgattgtcg gtacgctgat
    20101 gacgccaatt atcaacggca atttcgatgt gttgattaac accgaaggcg gacgcatgac
    20161 gttgctcggc gttctggtgg cgctgattgg cgtagggatt gtaactcgag ccgggcagtt
    20221 gaaagagcgc aagatgggca ttaaagccga agagttcaat ctgaaaaaag gactggtgct
    20281 ggcggtgatg tgcggcattt tctctgccgg gatgtccttt gcgatgaacg ccgcaaaacc
    20341 gatgcatgaa gccgctgccg cactaggcgt cgatccactg tatgtcgctc tgccaagcta
    20401 tgttgtcatc atgggcggcg gcgcgatcat caacctcggt ttctgtttta ttcgtctggc
    20461 aaaagtgaag gatttatcgc taaaagccga cttctcgctg gcaaaaccgc tgatcattca
    20521 caatgtgtta ctctcagcac tgggcgggtt gatgtggtat ctgcaattct ttttctatgc
    20581 ctggggccac gcccgcattc cggcgcagta tgactacatc agttggatgc tgcatatgag
    20641 tttctatgta ttgtgcggcg gtatcgtcgg gctggtgctg aaagagtgga acaatgcagg
    20701 acgccgtccg gtaacggtgt tgagcctcgg ttgtgtggtg attattgttg ccgccaacat
    20761 cgttggcatg ggcatggcga attaatcttt ctgcgaattg agatgacgcc actggctggg
    20821 cgtcatcccg gtttcccggg taaacaccac cgaaaaatag ttactatctt caaagccaca
    20881 ttcggtcgaa atatcactga ttaacaggcg gctatgctgg agaagatatt gcgcatgaca
    20941 cactctgacc tgccgcagat attgattgat ggtcattcca gtctgctggc gaaattgctg
    21001 acgcaaaacg cgctcactgc acgatgcctc atcacaaaat ttatccagcg caaagggact
    21061 tttcaggcta gccgccaacc gggtaatcag cttatccagc aacgtttcgc tggatgttgg
    21121 cggcaacgaa tcactggtgt aacgatggcg attcagcaac atcaccaact gcccgaacag
    21181 caactctgcc atttcgttag caaacggcac atgctgacta ctttcatgct caagctgacc
    21241 gataacctgc cgcgcctgtg ccatccccat gctacctaag cgccagtgtg gttgccctgc
    21301 gctggcgcta aatcccggaa tcgccccctg ccagtcaaga ttcagcttca gccgctccgg
    21361 gcaataaata atattctgca aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc
    21421 agcatgaatg taaaagagat cgccacgggt aatgcgataa gggcgatcgt tgagtacatg
    21481 cagaccatta ccgcgccaga caatcaccag ctcacaaaaa tcatgtgtat gttcagcaaa
    21541 gacatcttgc ggataacggt cagccacagc gactgcctgc tggtcgctgg caaaaaaatc
    21601 atctttgaga agttttaact gatgcgccac cgtggctacc tcggccagag aacgaaattg
    21661 attattcgca atatggcgta caaatacgtt gagaagattc gcgttattgc agaaagccat
    21721 cccgtccctg acgaatatca cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa
    21781 agtggttact gtcgctgaat ccgcaatgat aggcgatgtc agtaacgctg gcctcgctgt
    21841 gacgtagcag atgtcgggct ttcatcagtc gcaggcgatt caggtatcgc tgaggcgtca
    21901 gtcccgtttg ctgcttaagc tgccgatgta gcgtacgcag tgaaagagaa aattgatccg
    21961 ccacggcatc ccaattcacc tcatcggcaa aatggtcctc cagccaggcc agaagcaagt
    22021 tgagacgtga tgcgctgttt tccaggttct cctgcaaact gcttttacgc agcaagagca
    22081 gtaattgcat aaacaagatc tcgcgactgg cggtcgaggg taaatcattt tccccttcct
    22141 gctgttccat ctgtgcaacc agctgtcgca cctgctgcaa tacgctgtgg ttaacgcgcc
    22201 agtgagacgg atactgccca tccagctctt gtggcagcaa ctgattcagt ccggcgagaa
    22261 actgaaatcg atccggcgag cgatacagca cattagtcag acacagatta tcggtatgtt
    22321 catacagatg ccgatcatgg tcgcgcacaa aacagaccgt gccaccggtg atggtatagg
    22381 gctgcccatt aaacacatga atacccgtgc catgttcgac aatcacaatt tcatgaaaat
    22441 catgatgatg ttcaggaaaa tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt
    22501 taccagacgg aaaaaaatcc acactatgta atacggtcat actggcctcc tgatgtcgtc
    22561 aacacggcga aatagtaatc acgaggtcag gttcttacct taaattttcg acggaaaacc
    22621 acgtaaaaaa cgtcgatttt tcaagataca gcgtgaattt tcaggaaatg cggtgagcat
    22681 cacatcacca caattcagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca
    22741 atggcccatt ttcctgtcag taacgagaag gtcgcgaatt caggcgcttt ttagactggt
    22801 cgtaatgaaa ttcagcagga tcacattatg acctttcgca attgtgtcgc cgtcgatctc
    22861 ggcgcatcca gtgggcgcgt gatgctggcg cgttacgagc gtgaatgccg cagcctgacg
    22921 ctgcgcgaaa tccatcgttt taacaatggg ctgcatagtc agaacggcta tgtcacctgg
    22981 gatgtggata gccttgaaag tgccattcgc cttggattaa acaaggtgtg cgaggaaggg
    23041 attcgtatcg atagcattgg gattgatacc tggggcgtgg actttgtgct gctcgaccaa
    23101 cagggtcagc gtgtgggcct gcccgttgct tatcgcgata gccgcaccaa tggcctaatg
    23161 gcgcaggcac aacaacaact cggcaaacgc gatatttatc aacgtagcgg catccagttt
    23221 ctgcccttca atacgcttta tcagttgcgt gcgctgacgg agcaacaacc tgaacttatt
    23281 ccacacattg ctcacgctct gctgatgccg gattacttca gttatcgcct gaccggcaag
    23341 atgaactggg aatataccaa cgccacgacc acgcaactgg tcaatatcaa tagcgacgac
    23401 tgggacgagt cgctactggc gtggagcggg gccaacaaag cctggtttgg tcgcccgacg
    23461 catccgggta atgtcatagg tcactggatt tgcccgcagg gtaatgagat tccagtggtc
    23521 gccgttgcca gccatgatac cgccagcgcg gttatcgcct cgccgttaaa cggttcacgt
    23581 gctgcttatc tctcttctgg cacctggtca ttgatgggct tcgaaagcca gacgccattt
    23641 accaatgaca cggcactggc agccaacatc accaatgaag gcggggcgga aggtcgctat
    23701 cgggtgctga aaaatattat gggcttatgg ctgcttcagc gagtgcttca ggagcggcaa
    23761 atcaacgatc ttccggcgct tatctccgcg acacaggcac ttccggcttg ccgcttcatt
    23821 atcaatccca atgacgatcg ctttattaat cctgagacga tgtgcagcga aattcaggct
    23881 gcgtgtcggg aaacggcgca accgatcccg gaaagtgatg ctgaactggc gcgctgcatt
    23941 ttcgacagtc tggcgggcgc tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg
    24001 cggtgaagat ttctcgcaac tgcatattgt cggcggaggc tgccagaaca cgctgctcaa
    24061 ccagctatgc gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg aagcctcgac
    24121 gctcggcaat atcggcatcc agttaatgac gctggatgaa ctcaacaatg tggatgattt
    24181 ccgtcaggtc gtcagcacca ccgcgaatct gaccaccttt acccctaatc ctgacagtga
    24241 aattgcccac tatgtggcgc ggattcactc tacacgacag acaaaggagc tttgcgcatg
    24301 accactcaac tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc agtagggatt
    24361 gatgtcgagg aggcgctgcg ccaacttgat cgtttacccg tttcaatgca ctgctggcag
    24421 ggtgatgatg tttccggttt tgaaaacccg gaaggttcgc tgaccggagg gattcaggct
    24481 actggcaatt atccgggcaa agcgcgtaat gccagtgagc tacgtgccga tctggaacag
    24541 gctatgcggc tgattccggg gccaaaacgg cttaatttac atgccatcta tctggaatca
    24601 gacacgccag tctcgcgcga ccagatcaaa cctgagcact tcaaaaactg ggttgaatgg
    24661 gcgaaagcca atcagctcgg tctggatttt aacccctcct gtttctcgca tccgctaagc
    24721 gccgatggct ttacgctttc ccatgccgac gacagcattc gccagttctg gattgatcac
    24781 tgcaaggcca gccgccgcgt ttcggcctat tttggtgagc aactcggcac accgtcggtg
    24841 atgaacatct ggatcccgga tggcatgaaa gatatcaccg ttgaccgtct cgctccgcgc
    24901 cagcgtctgc tggcagctct ggatgaggtg atcagcgaga agctggatcc ggcgcaccat
    24961 atcgacgccg ttgagagcaa attgtttggc attggcgcag agagctacac ggttggctcc
    25021 aatgagtttt acatggggta tgccaccagc cgccagactg cgctgtgcct ggacgccggg
    25081 catttccacc cgactgaagt gatttccgac aagatttccg ccgccatgct gtatgtgccg
    25141 cagttgctgc tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg
    25201 gatgatgaaa cccaggcgat tgccagtgag attgttcgtc acgatctgtt tgaccgggtg
    25261 catatcggcc tcgacttctt tgatgcctct atcaaccgta ttgctgcgtg gatcattggt
    25321 acacgcaata tgaaaaaagc cctgctgcgt gcgttgctgg aacctaccgc tgagctgcgc
    25381 aagctggaag cggcgggcga ttacactgcg cgtctggcac tgctggaaga gcagaaatcg
    25441 ttgccgtggc aggcggtctg ggaaatgtat tgccaacgtc acgatacgcc agcaggtagc
    25501 gaatggctgg agagcgtgcg ggcatatgag aaagaaactt tgagtcgccg cgggtaaaca
    25561 ctgccggatg cggcacaagt gccttatccg gcctacaggc cggcaatagt tgtaggcctg
    25621 ataagacgcg acagcgtcgc atcaggcatt gattgccgga tgcggcacga gcgccttatc
    25681 cggcctacgg gtcgtgcatc tgacaacacc gaatttacag gaacacagaa catgcaaaac
    25741 attacccagt cctggtttgt ccagggaatg atcaaagcca ccaccgatgc ctggctgaaa
    25801 ggctgggacg agcgcaacgg cggcaacctg acgctgcgcc tggatgacgc cgatatcgcg
    25861 ctttatcacg acaacttcca cccacaaccg cgctatattc cgctcagcca acccatgcct
    25921 ttactggcaa atacgccgtt tattgtcacc ggctccggca aattcttccg taacgtccag
    25981 ctcgatcctg cagctaactt aggcgtcgta aaagtcgaca gcgatggcgc gggctaccat
    26041 attctctggg ggttaaccaa cgaagccgtt ccaacttctg aacttccggc tcactttctc
    26101 tcccactgcg agcgcattaa agccaccaac ggtaaagatc gggtgatcat gcactgccac
    26161 gccaccaacc tgatcgccct cacctatgta cttgaaaacg acaccgcggt cttcactcgc
    26221 caactgtggg aaggcagcac cgagtgtctg gtggtattcc cggatggcgt tggcattctg
    26281 ccgtggatgg tacccggcac ggacgaaatc ggccaggcaa ctgcccaaga gatgcaaaaa
    26341 cattcgctgg tgttgtggcc cttccacggt gtgttcggtt ccggaccgac gctggatgaa
    26401 accttcggtt taatcgacac cgcagaaaaa tcagcggaag tgttagtgaa ggtgtattcg
    26461 atgggcggca tgaaacagac tatcagccgt gaagagttga tagcgctcgg ccagcgtttc
    26521 ggcgttacac cactcgccag tgcgctggcg ctgtaaggag caaacatgat ccgcaaagcc
    26581 tttgtcatgc aggtaaaccc cgacgcccac gaagagtatc agcgtcggca taatcccatc
    26641 tggccggaac tggaagcagt gttgaaatct cacggtgcgc ataactacgc catttacctc
    26701 gacaaagcgc gtaatctgct gtttgccacg gtagagattg aatctgaaga acggtggaat
    26761 gcggtagcca gcactgaaat ctgccaacgt tggtggaaat atatgaccga tgttatgccc
    26821 gctaacccgg ataacagccc ggtgagtagc gagctgcaag aagtgtttta cctgccttaa
    26881 tgagttgcgg tgccggaaaa tatccagcac cgcgacttca tcagaagaag tatttaaagc
    26941 gaacgcgtgc gccgtaacgg tcatcagtat catcaccgtg ttttttatcg ccgtcgctat
    27001 ccagaatgga gtaatacgtc ccgaggtaaa tattaaagtt ctccatatcc atcacgttag
    27061 cgaactgata agacgcatga atggtgtgaa tggtgtacgt cccttcatca tcaatccagc
    27121 agtcgttatc acaaactaca ccgctgaatt catcaatctt attgtgcgcg tagatataac
    27181 cgagctcgaa acgtttccac agcgcgttaa tcccggcagt gaaatctttc tcattattag
    27241 catctaaata ggcggtattt agattaacca caatgccatt gtccggatcc gttttcaggc
    27301 cgttccaggt catgctcata ccatagccgg tacgatcgga ctgatcgaca aaattaccct
    27361 tgctatcggt ataaccataa gcattattta ccacgttcgc ttccatcgct gcggaaacgg
    27421 tgaattcttc cgtcggcgac caggcaatta ctgggcgcag ataagcaaca tttttctgct
    27481 gctccatatc gcgtccatga taattaccgt cattatataa agatgttccg tcttcaagta
    27541 acgtgtttaa ttcaaaatac cagttatcga gttgtttgct gacgaggaaa ttaccgccag
    27601 cgttagagcg cccgcggccc tctttcatca tatagatata accgctgcca tcgtcataaa
    27661 gatcgttcgc agtattaccg gaatgttcaa caaaggtatc ctgattcaat gggaacatat
    27721 cgtaggcttc aaaacggcct actttgacct tccagtcgtt ttctttgcca aagaagaata
    27781 ccgcatcatc gatatttact gaaccgtgca tatcacccag cggttgtgcg gagaacccgg
    27841 cgaaataacc gttgtccatc tttcgcatac cgtcaaaacc taacagaata cgaccattga
    27901 gattccactg ttcattagtg ggatcgctat tcacatcagc gttggtcatt gccagcaggc
    27961 catgattact ttccgcatcc atattaaatt ctacgtcgcc gtagattttt aaatcgccat
    28021 aacctgaaag caaaagatta ggtgaggtgg catttttctt tgcagattcc gccgttgcct
    28081 gtccatttac cggcgtattg ccctgagcgg ccttaatttc gcggatctca gcggcttgtt
    28141 gtgtctgcag ctgtttaacc tggctctctg cttttgccgc ccgcatttct gccgcttcca
    28201 ggcgttcttc cagcaatgcc atgcgttgtt ccagcattaa atgctgtgtt tttgccatac
    28261 cggtactaca tgccaataat aaaccgatgg ctaaagataa tgtcgatttt ttcattattt
    28321 acgttccctg aaataaagga gaccctacgc tttccaaaag gaatacgtat atagagaaat
    28381 ataaggtgtg cactttcgaa tttttaatcc aacttcagca ggccgatgga tagtatcccg
    28441 ccgcataata tgagtaaatc cgctgatttc ctggaacgtg aggaatataa caatagtgcc
    28501 gtttttaact aaatatcgta aaaaatatta gatatgattc aagctggctc ataagccaca
    28561 aatataaata acgtttagac aaattatctg agacatttaa acattcacaa aatcattaaa
    28621 taataactct ttattcacaa gatttatttt acctactaat atgtgcctta cgccgcaaaa
    28681 tttatccttc actctgcttt atattgtcac gacttcacga catatccgcg ctcatcacaa
    28741 acccgccccg ctattacaat taactgaaat attttttcat ggagtccgat taatggcagc
    28801 tcttactgca agctgtattg acctgaatat tcagggcaat ggcgcttatt ccgttctgaa
    28861 gcagttggcg acaatggcgt tacaaaacgg ttttatcacc gactcacacc agttcctgca
    28921 aaccctgctg ctgcgcgaaa aaatgcactc caccggtttt ggttctggtg tcgccgtgcc
    28981 gcacggtaaa agcacctgcg ttaaacaacc gttcgtgtta ttcgcccgca aagcgcaggc
    29041 tattgactgg aaagccagcg acggcgaaga cgtcaattgc tggatctgcc tcggcgtacc
    29101 gcaaagtggc gaagaggatc aggtcaaaat catcggcaca ctgtgtcgca aaattattca
    29161 ccaggaattt attcatcaac tgcaacaggg cgataccgac caggtgcttg ccttgttaaa
    29221 tcaaaccctc agctcataag gaagtggcga tggagtcatc cttacgtatt gtcgcgatca
    29281 ccaactgccc cgccgggatc gctcacacct acatggtggc ggaagccctg gaacagaaag
    29341 cccgttctct cggccatacc attaaagtgg aaactcaagg gtccagcggc gttgaaaacc
    29401 gcttatccag cgaagagatt gccgctgccg attacgtgat tctcgccacc gggcgtggcc
    29461 tgagcggtga cgatcgcgca cggtttgccg ggaaaaaagt ttatgagatt gccatctccc
    29521 aggcgctgaa aaatatcgat cagattttca gtgaattacc gacaaattcg cagctttttg
    29581 ccgcagatag cggcgtgaag ctaggcaaac aggaagtaca aagcggcagc gtgatgagcc
    29641 acctgatggc gggcgtctct gcggcgctgc cgtttgtcat cggcggtggt atcctggtgg
    29701 cgcttgccaa catgctggtg cagttcggtt tgccgtatac cgatatgtcg aaaggcgcgc
    29761 cgtcgtttac ctgggtagtt gaatccatcg gttaccttgg cttcaccttt atgatcccca
    29821 tcatgggcgc ttatattgcc tcgtcgattg ccgataaacc ggctttcgct ccggcgtttc
    29881 tggtttgcta tctggcgaac gacaaagcgc tgctcggcac ccagtcgggt gcgggattcc
    29941 tcggcgcggt ggtgctgggg ctggcgattg gctatttcgt cttctggttc cgtaaagtcc
    30001 gtcttggcaa agcattacaa ccgctgctcg gctccatgct tatcccattc gttactctgc
    30061 tggtttttgg cgtgctcact tactacgtta tcgggccggt gatgtccgac ctcatgggcg
    30121 ggctactgca cttcctgaat accattcctc catcaatgaa gtttgcggca gcgtttctgg
    30181 tcggtgcgat gctggcgttc gatatgggcg gccccatcaa taaaaccgcc tggttcttct
    30241 gcttctcact gctggaaaaa cacatttatg actggtacgc catcgtcggc gttgtccccg
    30301 tcgcggcggg tcttgcgacg ttcattgcgc ccaaactgtt tacccgacag gaaaaagagg
    30361 ctgccagtag tgccattgtg gtcggtgcca cagtcgcaac cgaaccggct attccttacg
    30421 ctctggcagc tccgttgccg atgattaccg ccaatacgct ggcgggcggc attaccggcg
    30481 tgctggtgat cgctttcgga atcaaacgtc tggcacctgg tttgggtatc tttgacccgc
    30541 tgatcggcct gatgtcgccg gtaggttcgt tttatctggt gctggcgatt ggcctcgcgc
    30601 tgaacatttc attcattatc gtcctgaaag gattgtggtt acgccgcaaa gcgaaagccg
    30661 cgcaacagga gcttgtccat gaacattgag ttactgcaac agttgtgcga agccagcgcc
    30721 gtcagcggcg atgaacagga agttcgcgac attctgataa acacgctgga accttgcgtt
    30781 aatgagatca cctttgatgg tctgggcagc tttgttgccc gtaagggaaa taaaggtcca
    30841 aaagttgccg ttgtcgggca tatggatgaa gtcggcttta tggtcaccca catcgacgag
    30901 agcggttttc tgcgctttac caccattggc ggctggtgga atcagtcgat gctcaaccac
    30961 cgggtaacaa tacgcacaca caagggagtg aaaatccctg gtgtgattgg ttccgtagcg
    31021 cctcatgcgt taacggaaaa gcaaaagcaa caaccgctgt catttgatga gatgttcatt
    31081 gatattggcg cgaacagtcg cgaagaagcg aaaaaacgcg gtgcggaaat tggcgatttt
    31141 attagcccgg aagccaattt tgcctgctgg ggcgaagata aaatagtcgg caaggcgctg
    31201 gataatcgca tcggctgcgc gatgatggct gagctactac agacagtaaa taacccagga
    31261 attacgctgt acggcgtcgg cagcgtggaa gaagaagttg ggctacgcgg ggcacaaacc
    31321 tcggctgaac acactaaacc ggatgtggtg atcgtgctgg ataccgccgt cgcgggcgat
    31381 gttccgggca ttgataacat taaatacccg ctgaaactgg gcaacgggcc ggggctgatg
    31441 ctgtttgaca agcgctactt acccaaccag aaactggtgg cggcgttaaa aaactgtgcc
    31501 gcacataacg gtttaccgct gcaattttcc accatgaaaa ccggagcgac ggatggcggg
    31561 cgctacaacg taatgggcgg agggcgtccg gttgtcgcgc tgtgtctgcc aactcgttat
    31621 ctgcacgcta acagcggtat gatttcaaaa gccgattatg atgctctgct cacgctgata
    31681 cgggattttc tgacgacctt aactgcggag aaagtcaacg cgtttagcca gttccgtcag
    31741 gtggattaat gctaaacgaa cgccagttaa agattgtcga tctgctggag caacagccgc
    31801 gcacgcctgg cgagctggcg caacagactg gcgtttcagg caggaccatc ctgcgtgata
    31861 ttgactatct caactttacc cttaacggca aagctcgtat ctctgccagt ggcagtgcgg
    31921 gctatcagtt ggaaatcttc gagcgccgca gcttttttca gttgctgcaa aagcacgata
    31981 acgacgatcg gctgctggcg ctgttattac tgaatacctt tactccccgt gcgcaactcg
    32041 cctcggcgct taatttgcca gaaacttggg tcgcagagcg tctgccccgg ttaaaacagc
    32101 gttatgaacg cacttgttgc ctggccagtc gtcccggttt gggccatttc attgatgaaa
    32161 cagaagagaa acgcgttatc ttgctggcga acttgctacg caaagatccg tttttaatac
    32221 ctttggctgg cgtaacacga gacaaccttc agcatttatc cacggcctgc gacaaccaac
    32281 accgctggcc gctcatgcag ggggattatc tctccagcct gattctggcg atttacgccc
    32341 tgcgtaatca actgaccgat gagtggccgc aatatcccgg tgacgagata aaacaaatcg
    32401 ttgaacataa cggtctgttt cttggtgata acgctgtaag aaccctgaca ggtttgatag
    32461 agaaacagca tcagcaagcg caggtaattt ctgccgatca tgtgctgggg ttgctgcaaa
    32521 gggtgccagg tctcgcgtca ttgaatatta ttgatacgca gctggttgag aacattaccg
    32581 gtcatttatt acgttgcctt gccgcaccag tgtggattgc tgagcatcgc caaagcagca
    32641 tgaataacct gaaagccgcc tggcctgcgg cctttgatat gagtctgcac tttattacgc
    32701 tactgcgtga acagctcgat attccccttt tcgacagcga tctgatcggt ttgtattttg
    32761 cctgtgcgct ggaacgacat caaaacgaac gccagccgat cattttgctc tcggaccaga
    32821 acgcgattgc cactattaat cagctcgcca ttgagcgcga tgttttacat tgtcgggtga
    32881 ttattgcccg tagcttaagc gaacttgttg ccattcgcga agagattgag ccgttattga
    32941 tcattaacaa cagccattat ttactggatg acgcggtaaa taattacatc accgtaaaaa
    33001 atatcattac ggctgccggt atcgaacaaa taaaacattt tctggcgacg gcatttattc
    33061 gccagcaacc ggagcgtttt ttctctgccc ccggaagttt tcattattcg aatgtacgcg
    33121 gtgaaagctg gcaacatatt acccggcaaa tttgtgcgca attagtcgca caacaccata
    33181 ttaccgccga tgaagcgcaa cggatcatcg cccgcgaagg cgaaggtgaa aacctgattg
    33241 ttaatcgcct cgccatccca cattgctgga gcgaacagga gcgacgtttt cgtggatttt
    33301 ttattaccct cgcccaacca gttgaggtga ataacgaagt cattaaccat gtcttgatcg
    33361 cctgcgccgc cgccgatgcg cgtcatgagc taaaaatatt tagctatctg gcaagcgtat
    33421 tgtgtcagca tccggcagaa gttattgccg ggctaacagg atatgaggca tttatggagt
    33481 tacttcacaa ggggtgaact gtgtcggatg cgccgcatcc gacactgcat ttatcatcaa
    33541 aaacgtttaa cgcaggctat tatagtcatc aaccatttcg ttgtactcgc gtaacgctcg
    33601 tggaaatgag tcttcgacca tccagcttgt gtttgcatcc tgaagatgct ctttatccca
    33661 gtcagagtac gggactttat ctctgacgcg gcgaacatat tttttagtct cgagttgata
    33721 ctggtctgcc gaattaatga acgagaaatt catgccgcct ttatccgctt ctttggcctg
    33781 cgccaccagc gtttccagtt cagacacttt cttcattgcc gcatcaacat caaacttctc
    33841 ttgcgatatc aggttattaa tttgtttcgc tgaaatcatg actgccagag aatagtagtg
    33901 gaatgttttc ccttctcttt cttcaatatt tttcagttcc gcaagctggc gcttatcatt
    33961 aatttcctga atcgccgcat gataagattc tgctacaggt tcaaaggctt ccagattttt
    34021 taagaatgtc tggtgcagcg ttttcccttt cgcaaacgca tcgtctttat aattttcctg
    34081 ggtgtaatat ttatccattt cgttaatggt attacccaac gccacggcag catcgatata
    34141 actcaccgcc acgccatcaa ttggttgcag cgcaggcgtt aacgccacag cactttttac
    34201 gcctttttca cactcagcga ggttggattc actaatgccg tagatgccat aaacagtact
    34261 ctctttaccg gtaggcccct gtttaaaatc tttcagccag tcagcataac gcgccagact
    34321 gcgctgtacc gggatctgta acttgttgta gcacttgata tagacatcca ttttttcgtc
    34381 gaaaaccgct tgttcatcga ccagcggttg cgcaggtgtt tcaggtttag catctgcttt
    34441 tgcaacggga gcttcagtag gtttcgcttc aggagctggt gcggcaggtt gactattggc
    34501 aggcaggggc gtttctgttt cggctttttt atcatcacaa cccgtcagac cgagggccat
    34561 gatggcgact ataatagcgg aagataataa attccttttc a
//
LOCUS       DAZFBR010000042        34359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFBR010000042 DAZFBR010000000
VERSION     DAZFBR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.042"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..120
                     /locus_tag="ACHJZ2_002250"
     CDS             <1..120
                     /locus_tag="ACHJZ2_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO4568346.1"
                     /translation="YESPTSTHNDIMGGGGMSYAYEYDVKYNICHLRKKSRMR"
     gene            complement(207..882)
                     /locus_tag="ACHJZ2_002251"
                     /pseudo
     CDS             complement(207..882)
                     /locus_tag="ACHJZ2_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1027..1635)
                     /gene="yiiX"
                     /locus_tag="ACHJZ2_002252"
     CDS             complement(1027..1635)
                     /gene="yiiX"
                     /locus_tag="ACHJZ2_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO4568347.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1695..2012)
                     /gene="metJ"
                     /locus_tag="ACHJZ2_002253"
     CDS             complement(1695..2012)
                     /gene="metJ"
                     /locus_tag="ACHJZ2_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO4568348.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2289..3449
                     /gene="metB"
                     /locus_tag="ACHJZ2_002254"
     CDS             2289..3449
                     /gene="metB"
                     /locus_tag="ACHJZ2_002254"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO4568349.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3452..5884
                     /gene="metL"
                     /locus_tag="ACHJZ2_002255"
     CDS             3452..5884
                     /gene="metL"
                     /locus_tag="ACHJZ2_002255"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO4568350.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6023..6157)
                     /locus_tag="ACHJZ2_002256"
     CDS             complement(6023..6157)
                     /locus_tag="ACHJZ2_002256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568351.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6233..7123
                     /gene="metF"
                     /locus_tag="ACHJZ2_002257"
     CDS             6233..7123
                     /gene="metF"
                     /locus_tag="ACHJZ2_002257"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO4568352.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7452..9632
                     /gene="katG"
                     /locus_tag="ACHJZ2_002258"
     CDS             7452..9632
                     /gene="katG"
                     /locus_tag="ACHJZ2_002258"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO4568353.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9726..10631
                     /gene="yijE"
                     /locus_tag="ACHJZ2_002259"
     CDS             9726..10631
                     /gene="yijE"
                     /locus_tag="ACHJZ2_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO4568354.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(10658..11275)
                     /gene="yijF"
                     /locus_tag="ACHJZ2_002260"
     CDS             complement(10658..11275)
                     /gene="yijF"
                     /locus_tag="ACHJZ2_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO4568355.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11549..12652)
                     /gene="gldA"
                     /locus_tag="ACHJZ2_002261"
     CDS             complement(11549..12652)
                     /gene="gldA"
                     /locus_tag="ACHJZ2_002261"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO4568356.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12663..13325)
                     /gene="fsa"
                     /locus_tag="ACHJZ2_002262"
     CDS             complement(12663..13325)
                     /gene="fsa"
                     /locus_tag="ACHJZ2_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO4568357.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13337..15838)
                     /gene="ptsP"
                     /locus_tag="ACHJZ2_002263"
     CDS             complement(13337..15838)
                     /gene="ptsP"
                     /locus_tag="ACHJZ2_002263"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4568358.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGKAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16147..17226
                     /gene="frwC"
                     /locus_tag="ACHJZ2_002264"
     CDS             16147..17226
                     /gene="frwC"
                     /locus_tag="ACHJZ2_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO4568359.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17241..17561
                     /gene="frwB"
                     /locus_tag="ACHJZ2_002265"
     CDS             17241..17561
                     /gene="frwB"
                     /locus_tag="ACHJZ2_002265"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO4568360.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17612..19909
                     /gene="pflD"
                     /locus_tag="ACHJZ2_002266"
     CDS             17612..19909
                     /gene="pflD"
                     /locus_tag="ACHJZ2_002266"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO4568361.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19875..20753
                     /gene="pflC"
                     /locus_tag="ACHJZ2_002267"
     CDS             19875..20753
                     /gene="pflC"
                     /locus_tag="ACHJZ2_002267"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO4568362.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20755..21096
                     /gene="frwD"
                     /locus_tag="ACHJZ2_002268"
     CDS             20755..21096
                     /gene="frwD"
                     /locus_tag="ACHJZ2_002268"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO4568363.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21083..21934)
                     /gene="yijO"
                     /locus_tag="ACHJZ2_002269"
     CDS             complement(21083..21934)
                     /gene="yijO"
                     /locus_tag="ACHJZ2_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4568364.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22149..23882)
                     /gene="eptC"
                     /locus_tag="ACHJZ2_002270"
     CDS             complement(22149..23882)
                     /gene="eptC"
                     /locus_tag="ACHJZ2_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO4568365.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24065..26716)
                     /gene="ppc"
                     /locus_tag="ACHJZ2_002271"
     CDS             complement(24065..26716)
                     /gene="ppc"
                     /locus_tag="ACHJZ2_002271"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO4568366.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27068..28219)
                     /gene="argE"
                     /locus_tag="ACHJZ2_002272"
     CDS             complement(27068..28219)
                     /gene="argE"
                     /locus_tag="ACHJZ2_002272"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO4568367.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28373..29377
                     /gene="argC"
                     /locus_tag="ACHJZ2_002273"
     CDS             28373..29377
                     /gene="argC"
                     /locus_tag="ACHJZ2_002273"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO4568368.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29385..30161
                     /gene="argB"
                     /locus_tag="ACHJZ2_002274"
     CDS             29385..30161
                     /gene="argB"
                     /locus_tag="ACHJZ2_002274"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO4568369.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30222..31595
                     /gene="argH"
                     /locus_tag="ACHJZ2_002275"
     CDS             30222..31595
                     /gene="argH"
                     /locus_tag="ACHJZ2_002275"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO4568370.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            31863..32780
                     /gene="oxyR"
                     /locus_tag="ACHJZ2_002276"
     CDS             31863..32780
                     /gene="oxyR"
                     /locus_tag="ACHJZ2_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO4568371.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32763..34163)
                     /gene="sthA"
                     /locus_tag="ACHJZ2_002277"
     CDS             complement(32763..34163)
                     /gene="sthA"
                     /locus_tag="ACHJZ2_002277"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO4568372.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         7781 a         8664 c         9586 g         8328 t
ORIGIN      
        1 tatgaaagcc caacatcaac ccacaatgac ataatggggg gcgggggaat gtcatatgcc
       61 tatgaatatg atgtaaaata taatatatgt catttgagaa aaaagagcag aatgaggtaa
      121 aaatctttgg ctgtgtattc agtctggtaa tcatccaagg aaagctcatc agaagggtga
      181 tgactccaat tagtagaaca tatattttcg ataaaagcac cgatgacttt ttcatgaagt
      241 tctacagaac tcgagaaaca gatggttctg cgggccagac gtttgattcg ggttctcagg
      301 gtcagattgt tacgctcaat gtgctgagta aaaatcttac cagtcagatg cttctccttt
      361 ggtaactctc tagcgtaact accccattcg tcagtggtca acatgccgat ggtaaaaggg
      421 taagcagcgc cagcaattca cgacaggttt catcagtccg ggggccaaaa gtgtaggcca
      481 gaacaccacc agttttcgtg ttgtacacgt accagagcca gtgctgtcgc gctttactgc
      541 cgacaaagct ccattgctga ctaagtttgc agatgagtgc cacatcagca tgagcgaccg
      601 gggatgacgt tattcttcgt ggcgagagtt ttttaaagtt cggatgactg tattaatgcc
      661 gattttcagc gtacgggcag tatagcgaac cccggcaccg ttgaaagcca tttcagcgat
      721 ttggtcttta actcccggtt tacggggctc gtaacagtaa ctgagctgaa atacccggtg
      781 acagtcacgg ccgcggaatc gatcatgtct tttggggttc tgcccgcgac ggtaaacctg
      841 aacggactgg caacggggac aatgaaggtt aactgtgacc atgaaacatc cttaaaagcg
      901 agtattatat ccttaattca actaattgga ggcatcaccg tttaccggag cgacagcttg
      961 cggaaaggtc gtgcaactac aaagcaacaa acagactacg gcgggaatta tttttttcat
     1021 catccattac cacgaaaaca gcggccattc tttggcaacc gtggtgagtt gtggcgcatc
     1081 gaaaacggct tgtggtgaga ccaccgtttc ctcaagcgga atatttttgc cgtagcgttc
     1141 tttgagcttc gcctggacca gcgggttgct gagatcaaac tctttcaact tctgctgttc
     1201 gcccacacgc attcccagcg cattctgata aaccttccac accacttccg aacagtactg
     1261 gcgatcgtcg ctccatgaga agctgaaatc gtacggtttg ccgagataac gtttcgccgt
     1321 ttgcgccagt ttttgctgtt gttcaacact cagtccgcct tcaacgcggc gaacaacgta
     1381 tttgcccttt tcaccatggg caatccactg cttgagcggg gtgtatttca ccgggccgac
     1441 cgcttcaaaa acgtagggct ttttgttacg catcaccagc ataccggtgt ggctgtaatc
     1501 ggaatgggtc gccagttgga tcgctttact ttgcgatgag cgagagattt gaaagatgat
     1561 gtcgccagtt tgcggctgcc aggcaaaggc aggcacagaa accagcaggc tgaggatcag
     1621 tagcctgatt ttcatcgtta aacgtccttg tatgaaaaaa gcggctggtt aactccgcgc
     1681 cgctcttttc cgctttagta ttcccacgtc tccgggttaa tccccatctc acgcatgatc
     1741 tcttttgccg cttccgggat ttcgtcgctg cgctctttac gcagatcggc atcatccggc
     1801 aaaggttgcc cggtaaaggc atgcagaaac gcttcgcaca gcagctcgct gttggtagcg
     1861 tgacgcaggt tgttcacctg acgacgcgtg cgttcatcgg tgaggatttt taacacctta
     1921 agaggaatgg aaaccgtaat ctttttgact tgttcactct tcttgccgtg ctcagcgtat
     1981 gggctgatat attcgccgct ccattcagcc atgagatact taatcctctt cgtcaataaa
     2041 ttgagaccag accacacaat ttatgtgggt actgaccgta aacccgcata gtttaccgta
     2101 caggcgttac cgtgacatcg tgtaatgcac ctgtcggcgt gataatgcat ataattttaa
     2161 cggctatttg ggatttgctc aatctatacg caaagaagtt tagatgtcca gatgtattga
     2221 cgtccattaa cgcagtgttt actctggtgc ctgacatttc accgaccaag cccagggaac
     2281 ttcatcacat gacgcgtaaa caggccacca tcgcagtgcg tagcgggtta aatgacgacg
     2341 aacagtatgg ttgcgttgtc cctcccatcc atctttccag cacctataac tttaccggat
     2401 ttaatgaacc gcgcgcgcat gattactcgc gtcgcggcaa cccaacgcgc gatgtggttc
     2461 agcgtgcgct ggcagaactg gaaggcggtg ctggtgcagt acttactaat accggcatgt
     2521 ccgcgattca tctggtaacg accgtctttt tgaaacctgg cgatctgctg gttgcgccgc
     2581 acgactgcta cggcggtagc tatcgcctgt tcgacagcct ggcgaaacgc ggttgctatc
     2641 gcgtgttgtt tgttgatcaa ggcgatgaac aggcattacg ggcagcgctg gcagaaaacc
     2701 ccaaactggt actggtagaa agcccaagta atccattgtt acgcgtcgtg gatattgcga
     2761 aaatctgcca tctggcaagg gaagtcgggg cggtgagcgt ggtggataac accttcttaa
     2821 gcccggcatt acaaaatccg ctggcattag gtgccgatct ggtgttgcat tcatgtacga
     2881 aatatctgaa cggtcactct gacgtggtgg ctggcgtggt gattgctaaa gacccggacg
     2941 ttgtcactga acttgcctgg tgggcaaaca atattggcgt gacgggcggc gcgtttgaca
     3001 gctatctgct gctacgcggg ttgcgaacgc tggtgccgcg tatggagctg gcgcagcgca
     3061 acgcgaaggc gattgtgaaa tatctgcaaa cccagccgtt ggtgaaaaaa ctgtatcacc
     3121 cgtcgctgcc ggaaaatcag gggcatgaaa ttgccgcgcg tcagcaaaaa ggctttggcg
     3181 caatgttgag ttttgaactg gatggcgatg agcagacgct gcgccgtttc ctgggcgggc
     3241 tgtcgttatt tacgctggcg gaatcattag ggggagtgga aagtttaatc tctcacgccg
     3301 caaccatgac acatgcaggc atggcaccag aagcgcgtgc tgccgccggg atctccgaga
     3361 cgctgctgcg tatctccacc ggtattgaag atggcgaaga tttaattgcc gacctggaaa
     3421 atggcttccg ggctgcaaac aaggggtaaa aatgagtgtg attgcgcagg caggggcgaa
     3481 aggtcgtcag ctgcataaat ttggtggcag tagtctggct gatgtgaagt gttatttgcg
     3541 tgtcgcgggc attatggcgg agtactctca gcctgacgat atgatggtgg tttccgccgc
     3601 tggtagcacc actaaccagt tgattaactg gttgaaacta agccagaccg atcgtctctc
     3661 tgcgcatcag gttcaacaaa cgctgcgtcg ctatcagtgc gatctgatta gcggtctgct
     3721 acccgccgaa gaagccgata gcctcattag cgcttttgtc agcgatcttg agcgcctggc
     3781 ggcgctgctc gacagcggta ttaacgacgc agtgtatgcg gaagtggtgg gccacggcga
     3841 agtgtggtcg gcgcgcctga tgtctgcggt gcttaatcaa caagggctgc cagcggcctg
     3901 gcttgatgcc cgcgagtttt tacgcgctga acgcgctgcg caaccgcagg ttgatgaagg
     3961 gctttcttac ccgttgctgc aacagctact ggtacaacat ccgggtaaac gtctggtggt
     4021 gaccgggttt atcagccgta acaacgccgg tgaaacggtg ctgctggggc gtaacggttc
     4081 cgactattcc gcgacacaaa tcggtgcgct ggcgggtgtt tctcgtgtaa ccatctggag
     4141 cgacgtcgcc ggggtataca gtgccgatcc gcgtaaagtg aaagatgcct gtctgctgcc
     4201 attgctgcgt ctggatgaag ccagcgaact ggcgcgtctg gcggctcccg ttcttcacgc
     4261 ccgtacttta cagccagtat ccggtagcga aatcgacctg cagctgcgct gtagctacac
     4321 gccggatcaa ggctccacgc gcattgaacg cgtgctggcc tccggtactg gtgcgcgtat
     4381 tgtcaccagc cacgatgatg tctgcttgat tgagtttcag gtgcccacca gccaggattt
     4441 caaactggcg cataaagaga tcgaccagat cctgaaacgt gctcaggtgc gcccactggc
     4501 ggttggcgtg cataacgatc gccagttgct gcaattttgc tacacctcag aagtggccga
     4561 cagtgcgctg aaaatcctcg acgaagcggg attacctggc gaactgcgcc tgcgtcaggg
     4621 gctggcgctg gtggcgatgg tcggggcggg tgtcacccgt aacccgctgc attgccaccg
     4681 cttctggcag caactgaaag gccagccggt cgagtttacc tggcagtccg atgacggcat
     4741 cagcctggtg gcagtactgc gcaccggccc gaccgaaagc ctgattcagg gcctgcatca
     4801 gtccgtcttc cgcgcagaaa aacgcatcgg cctggtattg ttcggcaaag gcaatatcgg
     4861 ttcccgctgg ctggaactgt tcgcccgcga gcagagcacg ctttcggcgc gtaccggctt
     4921 tgaatttgtg ctggcaggcg tggtggacag ccgccgcagt ctgttgagtt atgacgggct
     4981 ggacgccagc cgcgcgttag ccttcttcaa cgatgaagcg gttgagcagg atgaagagtc
     5041 gttgttcctg tggatgcgcg cccatccgta tgatgattta gtggtgctgg acgttaccgc
     5101 cagccagcag ctggctgacc agtatcttga tttcgccagc cacggtttcc acgtcatcag
     5161 tgccaacaaa ctggcgggag caagcgacag caataaatat cgccagatcc acgacgcctt
     5221 cgaaaaaacc gggcgtcact ggctatacaa cgccaccgtc ggtgcgggct tgccgatcaa
     5281 ccataccgtg cgcgatctga tcgacagcgg cgatactatt ttgtcgatca gtgggatctt
     5341 ctccggcacg ctctcctggc tgttcctgca attcgacggt agcgtgccgt ttaccgagct
     5401 ggtggatcag gcgtggcagc agggcttaac cgagcctgat ccgcgtgacg acctctccgg
     5461 caaagacgta atgcgcaagc tggtgattct ggcgcgtgaa gcgggttaca acattgaacc
     5521 ggaccaggta cgtgtggaat cgctggtgcc tgctcattgc gaaggcggca gcatcgacca
     5581 tttctttgaa aatggcgatg aacttaacga gcagatggtg caacggctgg aagcggcccg
     5641 cgaaatgggg ctggtgctgc gctacgtggc gcgtttcgat gccaacggca aagcgcgtgt
     5701 gggcgtggag gcggtgcgtg aagaccatcc gctggcatca ctgctaccgt gcgataacgt
     5761 ctttgccatc gaaagccgct ggtatcgcga caacccgctg gtgatccgtg ggcctggcgc
     5821 ggggcgcgac gtcaccgccg gggcgattca gtcggatatc aaccggctgg cacagttgtt
     5881 gtaatttcag aaatttaata atgcccggta ctcatgtttt cgggtttatg gtttctaatg
     5941 aaatatattg aattatcata ggattaggcc ggatgaagcg tttacgacga atccggcaag
     6001 aagcaataag tacatggtta gtttatattt gcagtccggt ttgctttgca taccggattt
     6061 tctttttctt accatcctga agttttttca tcttccctga tttttcctca ccatcattgg
     6121 tcatttttcg gttgacgccc ttcggctttt ccttcatctt tacatctgga cgtctaaacg
     6181 gatggatgtg cacaacacaa catataacta caagcgattg atgaggtaag gtatgaactt
     6241 ttttcacgcc agccagcggg atgccctgaa tcagagcctg gcagaagtcc aggggcagat
     6301 taacgtttcg ttcgagtttt tcccgccgcg taccagtgaa atggagcaga ccctgtggaa
     6361 ctccatcgat cgccttagca gcctgaaacc gaagtttgta tcggtgacct atggcgcgaa
     6421 ctccggcgag cgcgaccgta cgcacagcat tattaaaggc attaaagatc gcactggtct
     6481 ggaagcggca ccgcatctta cttgcattga tgcgacgccc gacgagttgc gcaccattgc
     6541 acgcgactac tggaataacg gtattcgtca tatcgtggcg ctgcgtggcg atctgccgcc
     6601 gggaagtggt aagccagaaa tgtatgcttc tgacctggtg acgctattaa aagaagtggc
     6661 agatttcgat atctccgtgg cggcgtatcc ggaagttcac ccggaagcaa aaagcgccca
     6721 ggcggatttg cttaacctga aacgcaaagt cgatgcagga gccaaccgcg cgattactca
     6781 gttcttcttt gatgtcgaaa gctacctgcg ttttcgtgac cgctgtgtgt cggcgggcat
     6841 tgatgtggaa atcattccgg gcatattgcc ggtatctaac tttaaacagg cgaagaaatt
     6901 tgccgacatg accaacgtgc gtattccggc gtggatggcg caaatgtttg acgggctgga
     6961 tgacgatgcc gaaacccgca agctggtggg cgcgaatatt gccatggata tggtgaagat
     7021 tttaagccgt gaaggagtga aagatttcca cttctatacg cttaaccgcg ctgaaatgag
     7081 ttacgcgatt tgccatacgc tgggggttcg acctggttta taaatagtgt ggcttttatg
     7141 aaaatcacac agtgatcaca aattttaaac agagcacaaa atgctgcctc gaaatgaggg
     7201 cgggaaaata aggttatcag ccttgttttc tccctcatta cttgaaggat atgaagctaa
     7261 aacccttttt tataaagcat ttgtccgaat tcggacataa tcaaaaaagc ttaattacga
     7321 tcaatttgat ctacatctct ttaaccaaca atatgtaaga tctcaactat cgcatccgtg
     7381 gattaattca attataactt ctctctaacg ctgtgtatcg taacggtaac actgtagagg
     7441 ggagcacatt gatgagcacg tcagacgata tccataacac gacagccact ggcaaatgcc
     7501 cgttccatca gggcggtcac gaccagagcg cgggggcggg cacaaccact cgcgactggt
     7561 ggccaaatca actccgtgtt gacctgttaa accaacattc taatcgttct aacccgctgg
     7621 gtgaggactt tgactaccgc aaagaattca gcaaattaga ttactacggc ctgaaaaaag
     7681 atctgaaagc cctgctgaca gaatctcagc cgtggtggcc agccgactgg ggcagctacg
     7741 ccggtctgtt tattcgtatg gcctggcacg gcgcggggac ttaccgatca atcgatggac
     7801 gcggtggcgc gggtcgtggt cagcaacgtt ttgcaccgct gaactcctgg ccggataacg
     7861 taagcctcga taaagcgcgt cgcttgttgt ggccaatcaa acagaaatat ggtcagaaaa
     7921 tctcctgggc cgacctgttt atcctcgcgg gtaacgtggc gctggaaaac tccggcttcc
     7981 gtaccttcgg ttttggtgcc ggtcgtgaag acgtctggga accggatctg gatgttaact
     8041 ggggtgatga aaaagcctgg ctgactcacc gtcatccgga agcgctggcg aaagcaccgc
     8101 tgggtgcaac cgagatgggt ctgatttacg ttaacccgga aggcccggat cacagcggcg
     8161 aaccgctttc tgcggcagca gctatccgcg cgaccttcgg caacatgggt atgaacgacg
     8221 aagaaaccgt ggcgctgatt gcgggtggtc atacgctggg taaaacccac ggtgctggtc
     8281 cgacatcaaa tgtaggtcct gatccagaag ctgcgccgat tgaagaacaa ggtttaggtt
     8341 gggcgagcac ttacggcagc ggcgttggcg cagatgccat tacctctggt ctggaagtgg
     8401 tctggactca gacgccgacc cagtggagca actatttctt cgagaacctg ttcaagtatg
     8461 agtgggtaca gacccgcagt ccggctggcg caatccagtt cgaagcggta gacgcaccgg
     8521 aaattatccc ggatccgttc gatccgtcga agaaacgtaa accgacgatg ctggtgaccg
     8581 acctgacgct gcgttttgat cctgagttcg agaagatctc tcgtcgtttc ctcaacgatc
     8641 cgcaggcgtt caacgaagcc tttgcccgtg cctggttcaa actgacgcac agggatatgg
     8701 ggccgaaatc tcgttacatc gggccggaag taccgaaaga agatctgatc tggcaagatc
     8761 cgctgccgca gccgatctac aacccgaccg agcaggacat tatcgatctg aaattcgcga
     8821 ttgcggattc tggtctgtct gttagtgagc tggtatcggt ggcttgggct tctgcttcta
     8881 ccttccgtgg tggcgacaaa cgcggtggtg ccaacggtgc gcgtctggca ttaatgccgc
     8941 agcgcgactg ggatgtgaac gccgcagccg ttcgtgctct gcctgttctg gagaaaattc
     9001 agaaagagtc tggtaaagcc tcgctggcgg atatcatcgt gctggcaggt gtggttggcg
     9061 ttgagaaagc cgcaagcgcc gctggtttga gcattcatgt accgtttgcg ccgggtcgcg
     9121 ttgatgcgcg tcaggatcag actgacattg agatgttcga actgctggag ccaattgctg
     9181 acggtttccg taactatcgc gctcgtctgg atgtttccac caccgagtca ctgctgattg
     9241 ataaagccca gcaactgacc ctgaccgcgc cggaaatgac cgcgctggtg ggcgggatgc
     9301 gtgtactggg tgccaacttc gatggcagca aaaacggcgt cttcactgac cgcgttggcg
     9361 tattgagcaa tgacttcttc gtgaacttgc tggatatgcg ttacgagtgg aaagcgaccg
     9421 acgaatcgaa agagctgttc gaaggccgtg accgcgaaac cggcgaagtg aaatacactg
     9481 ccagccgtgc ggatctggta tttggttcta actctgtcct gcgtgcggtg gctgaagttt
     9541 acgccagcag cgatgctcac gagaagtttg ttaaagactt cgtggcggca tgggtgaaag
     9601 tgatgaacct cgaccgtttc gacctgctgt aatctgacct tcttcagcga ctgcctttca
     9661 ggcagtcgct gaagtttctt taccggcgta tagtgtccac aggaaaacta cacactggat
     9721 ctctcatgtc tgccgcagga aagagcaacc cactggcaat cagtggcctg gttgtgctca
     9781 cacttatctg gagttatagc tggattttca tgaagcaagt caccagctac atcggtgcct
     9841 tcgactttac cgccttacgc tgcattttcg gcgctctcgt tttattcatc gtccttttat
     9901 tacgtggtcg cggaatgcgc ccgacaccgt ttaaatacac cttagccatt gccctgttac
     9961 aaacctgcgg gatggttggc ctggcgcagt gggcgctggt cagcggcgga gcggggaagg
    10021 tggcgatcct gagctatacc atgccgttct gggtggtgat tttcgccgcg gtgtttctcg
    10081 gtgaacgcct gcgacgtggg caatatttcg cgattctgat tgccgctttc ggcttatttt
    10141 tggtgttgca gccgtggcaa ctcgatttct cttcgatgaa aagtgccatg ctggcaatcc
    10201 tctccggtgt tagttggggg gcgagcgcga ttgttgctaa acgtctgtat gcccgtcatc
    10261 cgcgcgtgga tttattgtcg ttaacatcct ggcagatgct ctatgcggcg ctggtgatga
    10321 gtgtggtcgc tttactggtg ccgcaacgtg aaattgactg gcagcccact gtgttctggg
    10381 ctctggccta cagtgcgatt ctggcgacgg cgttggcgtg gagcttatgg ctgtttgtgc
    10441 tgaaaaactt gcctgccagt attgccagct taagcacact ggccgttccc gtttgcggcg
    10501 tactcttttc ctggtggctg ctcggtgaga atccgggggc cgttgaaggt agcggtattg
    10561 tgctgattgt gctggcactg gcgctggtga gccgtaagaa aaaagaagcc gttagtgtaa
    10621 aaaggatctg aattttttct tcatgtgggg cgatcgctta tttaacaaaa taacgataat
    10681 gccccaccat gcgccagcta aacagcacat cttcttcctg cgcacctgcg ccaatgttat
    10741 gtatcaccag cggcgtaccg tcgcgggcga agccatctga aaccacccca atatgtgcca
    10801 gcccgttatc taatcgccag gagacaatat cgcccgcttg atagtcactg gggttcttgc
    10861 tgatggggcg tgttttatcg tggcgggtaa accaggtttc cagattaggc acccgacggt
    10921 gatcgatgtt gctgtccggg cgctttaact gccatttttg cgggtactcg gcaaaattct
    10981 tcgccatatc ttcgtgcacc agtttctgca aatcgacctt ctggctgcgc aatgcacgga
    11041 tcaccacatc ggaacatacc ccgcgttctt gcgggacatc accgccagga taggtaagct
    11101 gcacatacgc cggatcgtaa aatagcgtgc tgccgatttg ctgtctggca ccgtctgcga
    11161 tggcaaggtt ggtattggcc tggatttgta ccacggttgg tggaatggcg ggagatttta
    11221 aggagtagct ggtaaatgcc gttagcaggc tgaccagcgc cagtgaagct ttcatctgtt
    11281 tcgtccgtga aaataagagt cacagattcg accttccggg ctaacgattg ttctgtagag
    11341 aaaatgtcca gaagcggata aaaaattcct gaacaacgtg ggagtttcag attactggca
    11401 cagagtgccg gatgcggcgt gaacgtctta tctggcctaa aaagcacgca aattcaatat
    11461 attgcagaga ttgcgtaggc ctgataagcg tagcgcatca ggcaattttg cgttcaaact
    11521 cccggacaag ccgggagttt ggagtaggtt attcccactc ttgcaggaaa cgctgaccgt
    11581 actggtcggc taccagcaga gcggcgtaaa cctgatctgg cgtcgcgccg ccaggcatgt
    11641 tgtggatggt ttcaccttct gcacatgccg cttctgccac aattcgcatt ttcgccggga
    11701 catcttcttt aatatccagt tgagcgagag ttattggcaa acctaccgca tggctaagcg
    11761 cagctacggt ttcgatttcc tcaaccggcg agttttccag taccagctgc gtcagtgtac
    11821 caaatgccac tttttcaccg tgataatagt gatgggcatc aggaatggca gtcaggccat
    11881 tatgcaccgc gtgcgctgcc gccagaccac cgctttcaaa gccaacaccg cttaaataag
    11941 tgttcgcttc aatcacgcgt tccagtgccg gagtcactac atgctgttcg gcggcgagca
    12001 tcgctttttc gccttcttcc agcaaggtgt tgtagcacag ttcagccagc gccagcgccg
    12061 cctgagtgca cttgccgccc gccatggtgg tcgcaccgct acgagagcag gcacgcgctt
    12121 caaaccaggt cgccagcgca tcgccgatac ccgccgctaa cagacgcgca ggtgcgcccg
    12181 cgacgatttt agtatcgaca atcaccatat tcgggttatt tggcagcagc agatagcggt
    12241 caaattcacc ttcgtcagtg tagataacgg aaagagcgct acaaggtgcg tcggttgaag
    12301 caatggtcgg ggcaatggcg accggaacac ccatgaaatg tgccagtgct ttggctgtat
    12361 cgagggtttt tccgccaccg ataccgagaa ttgcgccaca ctgcgcagtc tccgcgatgc
    12421 cacgcagacg gtcgatctca ttttgcgaac attcaccgcc aaacggcgca atctctacta
    12481 ccagtccagc atctttaaag cttttctcga cagtggattg agcaaaacct aaaacaaatt
    12541 tgtcacccac cactaaccag cgttctgcca gcggcttcag gtattcgccc agacgattaa
    12601 tcacatcagc gccctggatg tatttacccg gtgattgaat aatgcggtcc ataattgctc
    12661 ctttagagat gagtagtgcc aaatgcggca ttccagtcgt gttcgaactt ctctatagct
    12721 gactctaccg caggggtgtt gagcatttgt tgcgctacat ctaagggcag ggtgatggat
    12781 tcacatcctg ccagtaaaca gtccagcgcc tgacgcggcg ttttaaagct ggccgccagc
    12841 accatgcttt ctggtgcgtg catttctaac agcgcttgca gctcctgaac cgtacgaatg
    12901 ccgtccccgc cctgggcatc cacgcggtta acatacggag caacgtattt tgcccctgcc
    12961 agtgcggcta ataacccttg tgcggcgcta tatacagcag tgccaagtgt agtaataccc
    13021 tcttttttca gtattttaat tgctgccaga ccttcggaag tcaccgggat tttcaccaca
    13081 atgcccggaa tggcatcgcg cagacgcttc gcttcttcca ccatcccctg cgcgtcgcgg
    13141 ctcatggtct gagcaaacag aatgccctca tcaccaatcg ctttttgcag acgcggcagc
    13201 acttcccata tggactcctt gctggcagcg ataatgctcg ggttagttgt caccccggca
    13261 atcgggaata tgtgtgccag acgttcgact tctgcgacgt tagcggtgtc cagatacagt
    13321 tccatgatgt ttccttttac agttccagtt catgttgcag caggctggcg atagcgtcgg
    13381 cagaggcggc gttaaccagc gcgttacgga attcttcgtg catgatgcgg cgagcgaggc
    13441 gcgagaaaat gcgcatatgc tgatcgcccg cagcgtgttt gttcagggtt aacataatga
    13501 tgaattgcgc ttcatcatcg ccccagcgca ccggcgcttg cagacgcgct acgctgatgg
    13561 tggattgctc aatgtgttct gatttgctgt ggggaatggc aaaactaaag cccagaccgg
    13621 tagagaaaac ggcctcgcgc gcccacaagt cggcttccag tttacgcgga tagcggcagc
    13681 ggcccgccag cagcaggtta tcggtcatgc ctttgagcac ttcttctttg ctgcgccagt
    13741 cgctttccag tgtgatgcac tcggcggtga ccagcggtgc gtcttgttgg gtcatgcgga
    13801 attgcgccag caggtgttcc acttccagcg aagtacggca ggccattgcc tggttgagca
    13861 acttgcggca ctcacggcta tcaagttgcg ccatccgcgc tttcgccgcc ggaattgatg
    13921 gtgcgctcat gctgagttca tccagcccta agccgaccag caacggcagc acggaacctt
    13981 tcgctcccag ctcaccgcac agaccaatcc atttgccctg gcgatgcacc gcttgcacgg
    14041 cgtaatcgag cgcccgcaag aatgccggat tcaggctgtt gtagtgacga gtaaccttag
    14101 cgttatcgcg atccactgcc agcagatatt gcgtcaggtc attactacca atactaaaga
    14161 aatcaatctc ttcgcagcat tgatcgatga tgaacatcac cgacggcact tccagcatga
    14221 tgccgagctg gatcttctca tcaaacggaa tgtgttcgtt acgcagttgc tgtttggctt
    14281 ccgccagttt ttctttcacc cataagatct cttccattga ggagatcatc gggatcatga
    14341 ttttcaggct gccgtgagca gaggcacgga ggatcgaccg tagttgtgtg gtgaacagcg
    14401 aggcgtattc ttcataaata cgcaccgcgc gataaccgag gaacgggtta gcttctgccg
    14461 gaatgttcag ataatcaacg ggtttgtcac cgccaatatc catagtgcgc acaataatgc
    14521 tgcgtccgtt ggcggactcc agcgcttgac aaaaaatgtt gtacaactcg ctttcgcccg
    14581 gtgcgctggt gcgatccata tagagcattt cagtgcggaa caaaccgacg ccttccgcac
    14641 cattgccgaa tgcggcctgc gcttccacgg agtgagcgat gttagcggca atttcaatgc
    14701 ggataccgtc agcggtacgg gcttgttggg tcagccagac acgctgttgc tcacgcaggg
    14761 cgtcctgtac gcgggcttct tgctgataat aacgagctac ggctttccct ggctcaacca
    14821 caatcgcccc ggcgttgccg tcgatataaa tcgtttgatg ctgccacgga gtaagggcat
    14881 caatatctac accaaccagt gtcggaatgt tgaacgaacg ggcaagaatt acggtatgtg
    14941 aggtggtgcc gccacttttc agtaacagac ctttgaggtg atttttatcc agttcgagga
    15001 actggctggg ggttagttca tcagccatac aaatggcggg ctgcgtcagt ttgcccggtg
    15061 ccgggaagcg ttgctcaccg tagatttgct gaagcagctg gaagcagaca tcgcgtacgt
    15121 ccagggcacg ttcttgcagg tagctgctgc tggaacggga aaactcttca caaaagtgat
    15181 tcgcactggc gacaatcgct tcggcgcagc ttaatccggc gctgacgcct gccagtaaat
    15241 gttcgtgcag ggaagtatcg ccagccaggg atcggtgcgc ttccagaatc gcgctggtgg
    15301 caccgtcgct atccagcaga cgaaactcaa tgtttttcag taccagcgtc aggccgtttt
    15361 ccagtgcgga ttgctcggcg tcaacgtctt tggctgcggg aagattaccc agcgcattga
    15421 gatctaaaga agagatcggc gtcagaatgc cgcctgcgct accgctgcac acggtgcggg
    15481 cgcggataat ttgcggattc agattggtca gtgaaaccgg cagtggttcc agttcgtcag
    15541 atttaacttc cgccagcggc gcatcgcagt gggggaattc atcgcgcagc cattggctta
    15601 aacgctggtg cgcttcctgt tcgtcggccc cggaaatcag taactggcag ttatcgcccg
    15661 ccagcgtgtc ggtgccaatc agcgcaaggg cgcttttggc gttgcccttg cggtcagtgc
    15721 gcaggttatg ccactcaatt tgtgatgaaa aagtattaca cagcgtttca acgtggcttg
    15781 ccggacgtgc atgtacgccg ttaggtagct cacaaataaa ttccacaatc agggccattg
    15841 cctctctccc ggtacggttt tctgtttaca ggataagagc gcacggcaac ggcctgccat
    15901 gtgacaaatc tgccaaaagc tggacaaatg taatgtaacc gtcaatttgc gacgcgtctc
    15961 acaagacgcc gttttgcggc atgcttccgg attatcgcaa gttatgaggc ggatcgcatt
    16021 tttgtactga tattacaaaa gtccagtaaa tggccttttt atccactgtt tgctccgcct
    16081 gcgattgcct attgtttcct ccatcaaaaa tatggacatg ggctttcgca agcccgggag
    16141 cagcttatga atgagttggt gcagatcctg aaaaataccc gtcagcattt aatgacgggc
    16201 gtttcacaca tgattccctt cgtggtatcg ggcggtattt tgctggcggt ttccgtcatg
    16261 ttgtatggca aaggcgcagt gccggatgcc gtagccgatc cgaatctgaa aaaactgttt
    16321 gatatcggcg ttgcaggttt gacgctgatg gtgcctttcc tcgccgctta catcggttac
    16381 tccattgcag agcgttctgc gctggctccg tgcgctatcg gtgcctgggt tggtaacagc
    16441 tttggtgcgg gcttctttgg tgcactgatc gccgggatta tcggcggcat cgtggtgcat
    16501 tacctgaaga aaattccggt gcataaagtt ctgcgctcgg tgatgcccat cttcatcatt
    16561 ccgatcgtcg gcacactgat taccgcaggc atcatgatgt ggggtctggg cgagcctgta
    16621 ggggcgttga ccaacagcct gactcagtgg cttcagggga tgcagcaggg cagcattgtt
    16681 atgctggcgg tgatcatggg tctgatgctg gcgttcgata tgggcggtcc ggttaacaaa
    16741 gtggcctatg ccttcatgct gatttgcgtt gctcagggtg tttataccgt ggtggccatt
    16801 gctgccgtgg gtatttgtat cccgccgctg gggatggggc tggcgacgct gattggtcgt
    16861 aaaaatttct ccgcagaaga gcgcgaaacc ggtaaagcgg cgctggtgat ggggtgcgtt
    16921 ggggttactg aaggggcgat tcctttcgcc gcagccgatc cgttacgtgt aattccttcc
    16981 atcatggttg gttcagtttg tggtgcagta actgcggcgc tggtcggtgc gcagtgctat
    17041 gcaggctggg ggggtctgat tgtgctgccg gtggttgaag gtaagttggg ttatatcgca
    17101 gcagtggctg tcggggcggt tgtgacggca gtctgcgtga acgtgctgaa aagtctggcg
    17161 cgtaaaaatg gatcttctac tgatgaaaaa gaagacgacc tggatttgga ttttgaaatt
    17221 aattaattga ggaacgagcc atgacgaaaa ttattgcagt aaccgcgtgt ccatctggtg
    17281 ttgcccatac ttatatggct gcagaggcgc tggaaagtgc cgcgaaagcg aaaggctggg
    17341 aagtgaaagt agaaacccag ggctcgattg gtctggaaaa cgaactgact gcggaagacg
    17401 tggcgagcgc cgatatggtg attctgacta aagatatcgg catcaagttt gaagagcgtt
    17461 ttgctggcaa gaccatcgtg cgcgtcaaca tcagcgatgc ggtaaaacgt gctgatgcca
    17521 ttatgagcaa aattgaagct cacctggcgc aaaccgctta agttcttctc ccgctcgcaa
    17581 gggcgggttc gctttcccac aggagttcct catgacgaat cgtatctctc gcctcaaaac
    17641 tgcactgttt gccaataccc gtgaaatctc gctggagcgt gcgctgcttt ataccgccag
    17701 ccatcggcaa accgaaggcg aaccggtgat attgcgccgg gcgaaagcaa cagcgtatat
    17761 ccttgaacat gttgaaattt cgattcgtga tgaagaactg attgccggta accgcaccgt
    17821 aaaaccgcgc gccgggatta tgtcgccgga aatggaccct tactggctgc tgaaagagct
    17881 gggtcaattc ccgacgcgtc cgcaggaccg ttttgctatc agcgaagaag ataaacgtat
    17941 ctaccgcgaa gagttgttcc cgtactggga aaaacgttcg atgaaagatt tcatcaacgg
    18001 gcagatgacg gatgaagtaa aagccgcgac tagcacgcag attttcagca tcaaccagac
    18061 ggataaaggc caggggcaca ttattattga ttacccgcgc ctgctgaatc acgggctggg
    18121 ggagctggtg gcacagatgc agcaacattg tcagcaacag ccggagaatc acttttatca
    18181 ggcagcgctg ttactgctcg aagcctcgca gaaacacatt ttgcgttacg ccgaactggc
    18241 ggaaacgatg gcggcaagct gcactgatgc ccagcgtcgc gaagaactgt tgactattgc
    18301 ggagatctcc cgccataacg ccgaacataa accgcagacg ttctggcagg cgtgccagtt
    18361 attctggtac atgaacatca ttctgcaata cgaatccaac gccagttcgc tgtctttagg
    18421 gcgcttcgac cagtatatgt tgccgttcta tcaggcatct ttaacccagg gcgaagatcc
    18481 ggcgttcctg aaagaactgc tcgaatcttt atgggtgaaa tgcaacgaca ttgtgctgtt
    18541 gcgctccacc agcagcgcgc gttatttcgc aggtttcccg actggctata ccgcactgct
    18601 cggcgggtta accgagaacg gacgtagcgc ggtgaacgtg ctttcgttcc tctgcctcga
    18661 tgcctatcaa agcgtacaat taccgcaacc gaacctaggg gtgcgcacta acgccttgat
    18721 cgacacgccg ttcctgatga aaaccgcaga aaccattcgc ctcggcaccg gtattccgca
    18781 aatctttaac gatgaagtgg tggtgccagc gttcctcaac cgtggcgttt cgctggaaga
    18841 tgcgcgcgac tattccgtag tgggctgtgt ggaattatct attcccggca gaacctacgg
    18901 cttgcatgac atcgcgatgt ttaatctgct gaaagtgatg gaaatctgcc tgcatgaaaa
    18961 tgaaggcaat gctgcgctga cttatgaagg tttactggag cagatccgcg ccaagatcag
    19021 ccactacatc accctgatgg ttgagggcag caatatttgt gatatcggcc atcgcgactg
    19081 ggcacctgta ccgctgctct cgtcttttat cagcgattgt ctggaaaaag gtcgcgatat
    19141 taccgatggc ggcgcgcgtt ataacttctc cggcgtacag ggaattggta tcgccaacct
    19201 gagcgattct ctccatgcgt tgaaagggat ggtttttgat caacagcgtt taagttttga
    19261 cgaattgctg tcggtattaa aagccaactt tgcaacgcca gaaggcgaaa aagtccgcgc
    19321 tcgcttaatt aaccgctttg agaaatacgg taacgatatc gacgaggtgg gtaacattag
    19381 cgccgaactg ttgcgccact actgcaaaga agtggaaaaa taccagaacc cgcgcggcgg
    19441 ttacttcacg ccgggatcgt ataccgtttc tgctcacgtc ccgttgggat cggtggttgg
    19501 cgcgacgcca gacggtcgtt ttgccggaga acagttggca gacggcggct tgtcacccat
    19561 gctgggccag gacgcacaag ggccaacagc ggtactgaag tcagtcagta agctcgataa
    19621 cacgctgctg tctaacggta cgttgctgaa cgtgaaattc actccggcga ccctggaagg
    19681 tgaagcggga ttacgcaaac tggccgactt cttacgggcg tttacccagc ttaagttgca
    19741 gcatattcag tttaacgtgg tgaacgccga cacgttgcgg gaagcgcaac agcgcccaca
    19801 agattatgcc gggctggtgg tgcgcgttgc cggatacagc gccttctttg tcgaactgtc
    19861 gaaggagatc caggatgaca tcatccgccg gacagcgcat cagctgtaac gttgtggaaa
    19921 cgcgccgcaa tgatgtggcg cgcattttca acattcagcg ttattcactg aatgacggcg
    19981 agggcattcg tacggtggtc ttttttaaag gctgtccgca tctttgcccg tggtgtgcta
    20041 atccggagtc gatctccggc aaaatccaga cggtacgcag agaggcgaaa tgtctgcact
    20101 gtgcgaaatg tttgcgtgat gcggatgaat gcccctccgg ggcgtttgaa cggattggtc
    20161 gcgatatcag ccttgacgcg ctggaacggg aagtgatgaa agatgacatt ttctttcgca
    20221 cgtccggcgg cggcgtcacg ctttctggcg gcgaagtgtt aatgcaggcg gagtttgcta
    20281 cccgtttttt acagcggctg cggttgtggg gcgtgtcatg cgccattgaa actgccggag
    20341 acgcgccagc cagcaagctg ttaccgctgg cgaaattgtg cgatgaagtg ttgttcgatt
    20401 taaaaattat ggacgcgact caggcgcggg atgtggtgaa gatgaacctg ccacgcgtgc
    20461 tggagaatct gcgtttgctg gtgagtgagg gcgtcaacgt gatcccgcgt ttaccgctga
    20521 tccctggttt cacgctcagc cgggagaata tgcagcaggc gctggatgta ctgatcccgc
    20581 tgaaaatcag gcagatccat ctgttaccgt ttcatcagta cggcgaaccg aaataccgcc
    20641 tgctggggaa aacatggtcg atgaaagagg tgcctgcgcc gtcgtcagcc gatgtgacaa
    20701 cgatgcgcga aatggcagaa cgggccggat ttcaggttac cgtgggaggt taaaatggca
    20761 tacctggtgg cagtaaccgc ctgcgtaagc ggcgtggcgc atacatatat ggcggcggaa
    20821 cggctggaaa agttgtgcct gttagagaag tggggagtca gcattgaaac tcagggcgcg
    20881 ctgggaacgg agaatcgttt agcggacgag gatatccgtc gggcggatgt tgctctgttg
    20941 attacggata tcgagcttgc cggtgccgag cgatttgaac attgccgcta tgtgcaatgc
    21001 agcatctacg cattcctgcg tgagccgcag caggtaatga gcgcggtgcg caaagtgctt
    21061 tctgcgccgc agcaaaccca tcttattctg gagtagtcgg tttttctgtc agttggctgt
    21121 ggtactgccg gcgatattcc gacggcgagc gttcggtgtt tttacgaaac agacggcaga
    21181 agtagttgct gtcgacaaag ccgcaggcgt gcgccacttc ttttaccttc aggtcatagc
    21241 ctttcaataa cgtcttagcg tgctccagtc gcgtgtgatt caggtattcg ttaaaaccaa
    21301 tggcccccgt tttttgaaac aggtgcgaga gatagtttgg tgaaatatag aatgcctgtg
    21361 cgacagattc gcgggtaagc gcggaggcat agcgttcgtc gatataatcg cgaatagctt
    21421 cgaatagtgc ctggctgcgt gaggcggtct ggatttggct gccaagcaaa tcgcggcagt
    21481 ggctaagcag gctggcgacg ataagccttg ccgtttgctg ctcctgcggc tgcatttgca
    21541 tttcattgag cgtttgtagc agaaaagaac ctatgcgtgg gccgcgtcgg gcgacgtgtt
    21601 gcttcgccag attttgatat tgtttgccat cccattgcac gacgctgaag ccgagttgct
    21661 gtttgccaaa cagcacgcta aaggtagtag caggggcttg ccattgtgga aaattccagc
    21721 caccagcggc aacgtacagc acatcgccgg gaactaacgt tgctccagcg ccggtatcaa
    21781 caaactcacc ttccagcaca atttccagcc gtggaaaatc gacctgatac gcaagatcgg
    21841 gcaccgggcc gttactgctg gcaaagtaaa tctggcgcag ggacagcggg ccgttgatca
    21901 ggcgggagag cagatagctg acgtcgtgat acatgttatt cccttaggta tggagacggg
    21961 aaaacagtaa ctggcattat taaaaaaggc cacctgaaaa gtgtggcctg aggggagttc
    22021 gatgtaaaca ttgcggcagt tgtattgccg gatgtggcgt aaacgcctta tccagtctac
    22081 cgccgcgatc tgtaggccgg ataagacgcg tcaagcgtcg catccggcaa atagcgcctg
    22141 gctgataatt actgattacc tacctggtcg ccatacggca gcgtgtcgta atcgatcagc
    22201 gcgtttttct tgtacgggtt accaatccag cgggtatttt ctttgaactg cggattcacc
    22261 actgaacggg ttggatcgta accgtcgtaa gataagcccg ctaaatctga ccaggtgtgg
    22321 atcagttccg caaggctgta tttacgatca acatcctgcg agaaatcacg cggatgagtc
    22381 gcttgccatt tttccgacgt ccacagcagg aacggaatgg tgtacatatg acgcgtcggg
    22441 ttgtcctcat tacgcccctg ggttttatga ggcggcgtgt cgtaaacctc ttcaccgtgg
    22501 tcagagaagt aaaccaggaa accgttcggg tctgctgctt taaagtcttt aatcaggctg
    22561 gcaaccacat gatcgttata caggttagcg ttgtcataat cgttatatga ctccagctct
    22621 tccgcgttta atcccggcgg aacatgatcg gtattgccat caaacttgcc ctgattttcc
    22681 gggtagcggt atttgtattt gatatgcgta cccagcagat gaacgatgat cagtttcttc
    22741 ggcgcagggt ccttcagcac ttcctggaac ggcttcagca cgttagtgtc gtattcacgc
    22801 gcactctgcg tacgttgctg gttcatgtag tactgcttgt cggtctggcg cgaaaatacc
    22861 gtcagcatgg tattgcgggc ggtcatcgtc tgctggttgg tgatccagaa ggttttataa
    22921 cccgcctgtt tcatcatgtt catcagcgac ggctgcgtca gatacagatc cgggttcttt
    22981 tcattggcaa aggtcagcgc ctgttgcagg atttcaatgg tgtacggacg agacgtaact
    23041 acgttattaa acacggtcag attcggatcg gttttatgca gcgcattcag ctccggcgtg
    23101 gtttcacgcg gataaccgta cagactcatg cgtccacgct gggttgactc gccaatcacc
    23161 agcactaagg tgcgcggttc gttacccgat tcatctttga aattagccag cggcggcaag
    23221 gcattatttt cattcagtag ctttgttagc gagtttagtt gctgacgata ctgataatag
    23281 ccggtcagga attgccacgg tgcggcaggc tccatacgcg aggccaggtt atccaacgtt
    23341 ttctcaaacg gcttgttttt gataaacata ttcatggcga tcggatgcag aatcaagcca
    23401 taaagcaggg caaaagagac aacataacgc cacggctttg gaatatagac cgggcgcagg
    23461 cgtgtccaca gcagaactgc caccgccgta taggccagcg cgataagcac aattttcagg
    23521 ctgaaatact ggcttaaata ctcgctggct tcgttggtgt tggtttcgaa catcacaaac
    23581 agaacgctct gcgagaactc ctgaccgtag atgacgtagt agcacagcgc cgccagagag
    23641 gccgcccata gcaccacgcc gatcactgcg gcaataattt tgatccgctt cggaaagagg
    23701 aataccggaa ttaaccacag cgagctgaat aacagcgagt cgcgaatgcc gttggtgcca
    23761 ctataaccac tgatgtaaat aatggcctgt agcagagtag agaaaaacca aaagtagagc
    23821 aatgcccaac ccagggcttt ccagctaaaa agaggtttag cctggacttc tgtggaatgc
    23881 atagtaagaa cctgtcttga aaaaaatatc gccgaattta ccgacaattc cttaaggata
    23941 tctgaaggga tatccagaat ttgaataaaa tgcagacaga aatatattga aaacgagggt
    24001 gttagaacag aagtatttca gaaaaccctc gcgcaaaagc acgagggttt gcaggagagg
    24061 aagattagcc ggtattacgc atacctgccg caatcccggc aatagtgacc attaacgcct
    24121 gctcgacgcg aggatccggt tcctggcctt ctttttctgc ctggcgggag cggtgcagca
    24181 actcggcttg caatacgttc agcgggtcgg tgtaaatatt ccgtagctga atagactctg
    24241 caatccacgg cagatcggcc atcagatggg aatcgttggc aatcgccagc accactttga
    24301 tgtcttcttc ttgcagattg cgtaactctt tacctaacgg ccacagtgtt ttgtctacca
    24361 ggcgttggtc atagtattcc gccagccaca ggtctgcttt ggcgaagacc atctccagca
    24421 tgccgagacg cgtcgagaag aatggccaat cgcggcacat agcctccagc tcgctctgtt
    24481 tgccatcttc gaccactttt tgcagcgccg tacctgcacc cagccaggcg gggagcatca
    24541 gacggttttg cgtccaggcg aagatccacg gaatggcgcg tagtgactcg acgccgccgg
    24601 ttgggcgacg tttcgccgga cgtgaaccca acggcagttt gcccagttcc tgttccggcg
    24661 tagcggagcg gaagtaaggc acaaaatctt tgttttcacg tacgtagcca cggtagagat
    24721 cgcaggagat gactgatagt tcatccataa tgcgacgcca gctctctttc ggctcaggtg
    24781 gtggcagcag gttggcttcc agtatcgccc cggtgtaaag cgacaggctg ctgacggtga
    24841 tttctggcag accatattta aagcggatca tctcgccctg ttcagtcacg cgcaggccac
    24901 ctttcaggct tcctggcggt tgtgacagca gcgccgcatg agcaggtgcg ccgccgcgac
    24961 caatggaacc gccgcgaccg tggaacaacg tcagctcaat acccgctttt tcgcaggttt
    25021 tgattaatgc atcctgtgcc tgatattgcg cccaggacgc tgccatcacg cccgcatctt
    25081 ttgctgagtc ggaatagcca atcatcacca tctgtttgcc ctgaatcagg ccgcgatacc
    25141 agtcgatatt cagcaactga gtcatgacat cgttggcgtt gttcaggtca tcgagagttt
    25201 caaacagcgg agcaaccggc atcgcaaacc cgatacccgc ttctttcagc agcaggtgga
    25261 cagccagcac gtcggacggc gttttcgcca tcgagatcac gtaggcggca atggagcctt
    25321 gcggtgcttc ggcaatcacc tggcaggtat cgagcacttc gcgcgtttcg gcgcttggtt
    25381 gccagttgcg cggcagaagc ggacgtttgg agttcagttc gcggatcagg aacgcctgtt
    25441 tgtcggcctc tgaccagctt tcgtagtcgc cgataccgag gtagcgggtc agctcgccca
    25501 gcgcttcggt atgacgcgtg ctctcctgac ggatatcaat acggaccagc ggtacgccga
    25561 aacatttcac gcggcgcagg gtgtcgagca gatcgccgtt ggcgataata cccatgccac
    25621 acgcctgaag tgactggtag caagcgtaga gcggttccca cagttcttcg ttttgtgtca
    25681 gcaggccttc tggttttggc agttcttcgc ctttcaggcg cgcttccagc catgcctgtg
    25741 tcgccatcag gcgagaacgc aggtttttca tcagatagcg atacggttct gcggcacctt
    25801 cttcgccaac cagcgccagc agttcagggg tcgcttcaac catcgacagt tcagaaacca
    25861 gaacctgaat atctttcagg aacagatcgg tggctttcca gcggctgagc agcaggacgt
    25921 ggcgggtgat atcggcagtg acgttcgggt tgccgtcgcg gtcaccgccc atccacgagg
    25981 taaaacgtac cggaacaaat tcgacgggca gtttgtagcc gaggttctct tccagttgtt
    26041 cgttcagttc gcgcaggtaa ttcggtacgc cttgccacag gctgttttct actacggcaa
    26101 agccccattt ggcttcatct accgggcttg gacgcagctt acggatttca tcggtatgcc
    26161 atgactgggc gatcaactgg cgcaggcgac gcatcagctg gttgtgttcg tagtcagcga
    26221 tatctttgtt atcgagctgt tttaagcagg cgttcacttc caccattttg tggatcagtg
    26281 tacgacgggt aatttcggtt gggtgagccg tgaggaccag ttccagcgac agcgattcca
    26341 ctgctttttt gatggtgtct tcgctcagtt ccggctggtt tttcagttta cgcagggtgc
    26401 gggcgatcac ttccgggttg ctggcagctt cgcctttcgg cgaaatgctg tggtattgct
    26461 cggcggtgtt ggccaggttc aggaactgac taaacgcacg cgcaacgggc agcagctcgt
    26521 cgttcgacaa attttgtaag gtggtgagta actcctggcg gttagcatca ttgccagcgc
    26581 gtgaagattt ggacaactta cggatagttt ctacgcgttc aagaatgtgt tctcccaacg
    26641 catccttgat ggtttctccc agcactttgc cgagcatact gacattacta cgcaatgcgg
    26701 aatattgttc gttcatatta ccccagacac cccatcttat cgtttgatag ccctgtatcc
    26761 ttcacgtcgc attggcgcga atatgctcgg gctttgcttt tcgtcgtctt ttataaagcc
    26821 acgtaaaagc ggtgacgtca aatgctgcga aatcgcttca gcaaacgaat aaatagcagg
    26881 aatttacgtc attaaattca cgacgcttta aataagcgta acttatggga atgttaaaaa
    26941 atcgcctcaa gtaacaccaa aggtgtaggt cggataagat gtgcaagcat cgcatccgac
    27001 attattgcgg cactggagtt tggcaacagt gccggatgcg gcgcaagcgc cttattcggc
    27061 ctacgtttta atgccagcaa aaatggtgaa tgacctgggt tatcagttcg cgggtgggct
    27121 tgataaaccg tgtttccaga tattcatcag gctgatgagc ctgattaatt gagccaggcc
    27181 ccagaaccag cgtcgggcat aacgtttgaa taaacggcgc ttcggtacag taattcacca
    27241 cttcggtttt tgctccgagc aatttctcaa ccacttcaac cagttgatga ttcggtgggc
    27301 attcatagcc agggatcggc ggatgcagct cgtcgaccgt cagacgaccc ggccagcgtt
    27361 cgctcaccgg agccaatgca tcgttgagca aaccattaag ttcattgagt gtcatgccag
    27421 gcagcggacg aatatccata tgcaactcac accaagcgca aatacggtta gaagcgtcgc
    27481 caccgtgaat atgcccgagg ttgagcgtag ggtatggcac ggtaaacgct tcgtagtgat
    27541 aacgttcttt caggttatcg cgcaattgca aaatatgccc gatggcgtcg tgcattagtt
    27601 ctatagcgtt aactccgcgt gctggatcgc tggagtgccc cgactggccc tgaatacgga
    27661 tggcgttaga gatatgacct ttatgtgcgc gtaccggttg tagtgacgtc ggttcgccaa
    27721 tgatggcgca atccgggcgc agggcggtag tttcggcaaa ataacgcgct ccggccatac
    27781 tggtttcttc atcagcagtc gccagaatgt agagcggttt tttcagtttc gtgacgtcga
    27841 catcgcgtag cgcatcaagg ataaacgcaa aaaagccttt catgtcggcg gtgcctaagc
    27901 cgtaaagctt gccgtcatgc tccgtcagtg taaacggatc gcgcgtccag cgaccgtcat
    27961 caaatggcac cgtatcggta tgccccgcca gcaacaagcc gccagccccc tgtccggtac
    28021 ttgccagcat attaaatttg ttgcgagttc ctggtacagg ctgcacttcc acattgaagc
    28081 ctaaatcttt aaaccagtcc gccagcagag tgattaaatc tgcattgctt tgatcgagtg
    28141 cctcttccgt ggcgcttatt gaaggtgtgg caatcagagc acggtaaatc tcgataaatg
    28201 gcggtaattt gtttttcatt gttgacacac ctctggtcat gatagtatca atattcatgc
    28261 agtatttatg aataaaaata cactaacgtt gagcgtaata aaacccacca gccgtaaggt
    28321 gaatgtttta cgtttaacct ggcaaccaga cataagaagg tgaatagccc cgatgttgaa
    28381 tacgctgatt gtgggtgcca gcggctacgc tggcgcagag ctagtgacct gtgtaaatcg
    28441 ccatccgcat atgaacataa ccgctttgac tgtttcagcg caaagcaatg atgcgggaaa
    28501 gttaatctcc gatttgcatc cgcagctaaa aggcatcgtt gatctgccgt tgcagccgat
    28561 gtcggatatc agcgagttta gccctggggt ggacgtggtg tttctcgcca ccgcccatga
    28621 agttagccac gatttagcgc cgcagtttct tgacgcgggc tgcgtggtgt tcgacctttc
    28681 cggcgcgttt cgtgttaacg acgccacctt ctatgaaaaa tattacggct ttacccatca
    28741 atacccggaa ctgctggaac aggcagccta cggtctggcg gagtggtgcg gtaataaatt
    28801 aaaagaagcg aatttgattg cggtgccagg ctgttatccg acggcagcac agttggcgct
    28861 gaaaccgttg attgatgccg atcttcttga cctcaatcag tggccggtga tcaacgccac
    28921 cagcggcgtg agcggtgcag gacgtaaggc agcgatttca aatagttttt gtgaagttag
    28981 cctgcaaccg tatggcgtct ttacccatcg ccatcaacca gagatcgcca cacacctcgg
    29041 tgctgacgtt atcttcaccc cgcatctggg caatttcccg cgcggcattc tcgaaaccat
    29101 tacctgccgc ctgaaaccgg gtgtgaccca ggcgcaagtg gcgcaagcgt tacagcaggc
    29161 gtatgcccat aaaccgctgg tgcggctgta tgacaaaggc gttccggcgc tgaaaaatgt
    29221 cgttgggcta ccattttgcg atatcgggtt tgccgttcag ggcgaacatc tgattattgt
    29281 ggcgaccgaa gacaacttac tgaaaggcgc ggcggcacaa gcggtacagt gcgccaatat
    29341 tcgtttcggc tatgcggaaa cgcagtctct tatttaaggg tgcaatgatg aatccattaa
    29401 ttatcaaact gggtggcgta ctgctggata gtgaagaggc gctggaacgt ctgtttagcg
    29461 cactggtgaa ttatcgtgag tcacatcagc gtccgctggt gattgtgcac ggcggcggtt
    29521 gcgtggtgga tgagctgatg aaagggctga atctgccggt gaaaaagaaa aatggcctgc
    29581 gggtgacgcc tgctgatcag attgacatta tcaccggagc actggcggga acggcaaata
    29641 aaaccctgtt ggcatgggcg aagaaacatc agattgcggc cgtaggtttg tttctcggtg
    29701 acggcgacag cgtcaaagtg acccagcttg atgaagagtt aggtcatgtt ggactggcgc
    29761 agccaggttc gcctaagctt atcaactcct tgctggagaa cggttatctg ccggtggtca
    29821 gctccattgg cgtaacagac gaagggcaac tgatgaacgt caatgccgac caggcggcaa
    29881 cggcgctggc ggcaacgctg ggcgcggatc tgattttgct ctccgacgtc agtggcattc
    29941 tcgacggcaa agggcaacgc attgccgaaa tgaccgccgc gaaagcagaa caactgattg
    30001 agcagggcat tattactgac ggcatgatag tgaaagtgaa cgcggcgctg gatgctgcgc
    30061 gcacgttggg ccgtccggta gatatcgcct cctggcgtca tgcggagcag cttccggcac
    30121 tgtttaacgg tatgccgatg ggtacgcgga ttttagctta agttttgttg gccggaggcg
    30181 cagctttccg gcattgaatt tcaaaataag gaaacagagt tatggcactt tggggcgggc
    30241 gttttaccca ggcagcagat caacggttca aacaattcaa cgactcactg cgctttgatt
    30301 accgtctggc ggagcaggat attgttggct ctgtggcctg gtccaaagcc ctggtcacgg
    30361 tcggcgtgtt aaccgcagaa gagcaggcgc aactggaaga ggcgctgaac gtgctgctgg
    30421 aagatgttcg cgccaggcca caacaaatcc ttgaaagcga cgccgaagat atccatagct
    30481 gggtggaagg caaactgatc gacaaagtgg gccagttagg caaaaagctg cataccgggc
    30541 gtagtcgtaa tgatcaggta gcgactgacc tgaaactgtg gtgcaaagat accgttagtg
    30601 agttactgac ggctaaccgg cagctgcaat cggcgctggt ggaaaccgca caaaacaatc
    30661 aggacgcggt catgccaggt tacactcacc tgcaacgcgc ccagccggtg acgttcgcgc
    30721 actggtgcct ggcctatgtt gagatgctgg cgcgtgatga aagccgtttg caggatgcgc
    30781 ttaagcgtct ggatgtcagc ccgttaggtt gtggcgcgct ggcgggaacg gcttatgaaa
    30841 tcgaccgtga acagttagcg ggctggctgg gatttgcttc agcaacccgt aacagtctcg
    30901 acagcgtttc tgaccgtgac cacgtgctgg aactgctttc tgctgccgct atcggcatgg
    30961 tgcatctgtc gcgttttgct gaagatctga ttttctttaa caccggcgaa gcggggtttg
    31021 tggagctttc tgaccgcgtg acttccggtt catcattaat gccgcagaag aaaaacccgg
    31081 atgcgctgga gctgattcgc ggtaaatgcg gtcgggtgca gggcgcgtta accggcatga
    31141 tgatgacgct gaaaggtttg ccgctggctt acaacaaaga tatgcaggaa gacaaagaag
    31201 gtctgttcga cgcgctcgat acctggttgg actgcctgca tatggcggcg ctggtgctgg
    31261 acggcattca ggtgaaacgc ccgcgttgcc aggaagcggc gcaacagggt tacgcgaacg
    31321 ccactgaact ggcggattat ctggtggcga aaggtgtgcc gttccgcgag gcacaccata
    31381 ttgtgggtga agctgtggtg gaagccattc gtcagggcaa accgctggag gagctgccgc
    31441 tcaccgagtt gcagaaattt agtccggtga ttggtgaaga tgtctatccg attctgtcgc
    31501 tgcaatcgtg cctcgacaag cgtgcggcaa aaggcggcgt ctcaccacag caggtggcgc
    31561 aggcgattgc ttttgcaagg gcgcggttgg ggtaagaaca tttatatgta caaatttgag
    31621 cctggcttat cgccgggctt ttttatggca aaaaaaaagc ggatcctgga gatccgcaaa
    31681 agttcacgtt ggctttagtt attcgagttg agaaactctc gaaacgggca gtgacttcaa
    31741 gggttaaaag aggtgccgct ccgtttctgt gagcaattat cagtcagaat gcttgatagg
    31801 gataatcgtt cattgctatt ctacctatcg ccatgaacta tcgtggcgat ggaggatgga
    31861 taatgaatat tcgtgatctt gagtacctgg tggcattggc tgaacaccgc cattttcggc
    31921 gtgcggcaga ttcctgccac gttagccagc cgacgcttag cgggcaaatt cgtaagctgg
    31981 aagatgagct gggcgtgatg ttgctggagc ggaccagccg taaagtgttg ttcacccagg
    32041 cgggaatgct gctggtggat caggcgcgta ccgtgctgcg tgaggtgaaa gtccttaaag
    32101 agatggcaag ccagcagggc gagacgatgt ccggaccgct gcatattggt ttgattccca
    32161 cggttggacc gtacctgcta ccgcatatta tcccgatgct gcaccagacc tttccaaagc
    32221 tggaaatgta tctgcatgaa gcacagaccc accagttact ggcgcaactg gacagcggca
    32281 aactcgattg cgtgatcctc gcgctggtga aagagagcga agcattcatt gaagtgccgt
    32341 tgtttgatga gccaatgttg ctggctatct atgaagacca cccgtgggcg aaccgcgaat
    32401 gcgtaccgat ggccgatctg gcaggggaaa aactgctgat gctggaagat ggtcactgtt
    32461 tgcgcgatca ggcaatgggt ttttgctttg aagccggggc ggatgaagat acacacttcc
    32521 gcgcgaccag cctggagacg ctgcgcaaca tggtggcggc aggtagcggg atcactttac
    32581 tgccagcgct ggctgtgccg ccggagcgca aacgcgatgg ggttgtttat ctgccgtgca
    32641 ttaagccgga accacgccgc actattggcc tggtttatcg tcctggctca ccgctgcgca
    32701 gccgctatga gcagctggca gaggccatcc gcgcaagaat ggatggccat ttcgataaag
    32761 tgttaaaaca ggcggtttaa accgtttaac gcagctaccc gataggcttc cgccatcgtc
    32821 gggtagttaa aggtggtgtt gacgaagtac tcaatagtgt tgccgccacc tttctgttcc
    32881 ataatcgcct gaccgatatg aataatttcg gcagcgcgct cgccaaagca gtgaatgccc
    32941 agaatctctt ttgtttcccg atggaacaaa attttcagcg tgcccacgtt catgccgacg
    33001 atttgtgcac gtgccagatg tttgaactgg gcgcggccca cttcatatgg cactttcatc
    33061 gcggtcagct gctgttcggt tttgcccaca gagctgattt ccgggatggt gtaaatgccg
    33121 gtagggatat cttcaatcag atgcgcggtg gcttcgcctt ttaccagcgc ctgcgcggca
    33181 atgcgcccct ggtcataggc cgctgatgcc aggctcggat aaccaatcac gtcgcccacc
    33241 gcgtaaacgt gcggctgtgc ggtctgatac atgctgttga ccttcagctg tccgcgactg
    33301 tcagtttcca gcccaatgtt ctgtaacgcc agcgaatcgg tattaccggt acgaccgttg
    33361 gcatagagca ggcagtcagc tttcagtttt ttacccgact tcagatgcat gatcacaccg
    33421 tcgtcacagc cttcgatctt ctcgtactct tcgttgtgac gaatcactac gccactgttc
    33481 cagaagtgat aggagagaga atctgacatc tcttgatcga gaaatgccag caggcgatcg
    33541 cgggtgttga tcagatccac ttttacatcc ataccgcgga agatcgacgc atattcacag
    33601 ccgatcactc cagcaccata gataagtaca tggcgcggtt cgtggtgcat gctgagaatt
    33661 gagtcgctgt cgtaaatgcg tggatgggtg aaatcaacat ctgttggatg atatggacga
    33721 gagccgcagg caataacaaa tttttcagcg gttagtgttt caacgctgcc gtccgggcaa
    33781 tccagcgcca acgtatgctc gtcaacaaag cgagcgttcc cctgcaatat ttcacagtga
    33841 ttacgttcgt aaaatccctg acgcatgcgc gtttgttgat taatcacgtt atcggcatgg
    33901 ttaaggatat cggcaaaaga agagcggagc agtcgggaat ggtcgctgta tagtgggttt
    33961 tgattgaatt ctataatgcg gctgacggcg tgacggagag ctttcgacgg gatggtgccc
    34021 cagtgggtgc aaccgccgcc aacattttga taacgctcga taactgcgac gcgcgcacct
    34081 tgcttaacca ggcccattgc agcgccttcg ccgccggggc cggaacctat tactatggca
    34141 tcgtaatcgt aggaatgtgg catggtaggg cttacctgtt cttatacata aaagcaacag
    34201 aatggtaaca ttttatcgcg ggtaagccaa ttgatccccg tcattaatct ggctatagcg
    34261 tgagcggcct ttgctttgtc tgcctccttc ttaacccatt tattcactat taatcgcttt
    34321 ttattatggc tgatagtttt atcttgccga agcgtgctt
//
LOCUS       DAZFBR010000043        34045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFBR010000043 DAZFBR010000000
VERSION     DAZFBR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.043"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            41..1021
                     /gene="ydcK"
                     /locus_tag="ACHJZ2_002278"
     CDS             41..1021
                     /gene="ydcK"
                     /locus_tag="ACHJZ2_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO4568373.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENYVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(1016..1552)
                     /gene="rimL"
                     /locus_tag="ACHJZ2_002279"
                     /pseudo
     CDS             complement(1016..1552)
                     /gene="rimL"
                     /locus_tag="ACHJZ2_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(1615..1839)
                     /gene="ydcH"
                     /locus_tag="ACHJZ2_002280"
     CDS             complement(1615..1839)
                     /gene="ydcH"
                     /locus_tag="ACHJZ2_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO4568374.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(1979..3634)
                     /gene="opgD"
                     /locus_tag="ACHJZ2_002281"
                     /gene_synonym="mdoD"
     CDS             complement(1979..3634)
                     /gene="opgD"
                     /locus_tag="ACHJZ2_002281"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO4568375.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(3859..5202)
                     /gene="ydcJ"
                     /locus_tag="ACHJZ2_002282"
     CDS             complement(3859..5202)
                     /gene="ydcJ"
                     /locus_tag="ACHJZ2_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4568376.1"
                     /translation="MANSITADEIREQFLQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5419..6342
                     /gene="ydcI"
                     /locus_tag="ACHJZ2_002283"
     CDS             5419..6342
                     /gene="ydcI"
                     /locus_tag="ACHJZ2_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4568377.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(6380..8020)
                     /gene="trg"
                     /locus_tag="ACHJZ2_002284"
     CDS             complement(6380..8020)
                     /gene="trg"
                     /locus_tag="ACHJZ2_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO4568378.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGEPVSFAPV"
     gene            8419..8568
                     /gene="hokB"
                     /locus_tag="ACHJZ2_002285"
     CDS             8419..8568
                     /gene="hokB"
                     /locus_tag="ACHJZ2_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO4568379.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            complement(8546..8614)
                     /gene="yncP"
                     /locus_tag="ACHJZ2_002286"
     CDS             complement(8546..8614)
                     /gene="yncP"
                     /locus_tag="ACHJZ2_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO4568380.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(8640..8813)
                     /locus_tag="ACHJZ2_002287"
     CDS             complement(8640..8813)
                     /locus_tag="ACHJZ2_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568381.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(9058..9588)
                     /gene="cybB"
                     /locus_tag="ACHJZ2_002288"
     CDS             complement(9058..9588)
                     /gene="cybB"
                     /locus_tag="ACHJZ2_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO4568382.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            9777..10778
                     /gene="gap"
                     /locus_tag="ACHJZ2_002289"
     CDS             9777..10778
                     /gene="gap"
                     /locus_tag="ACHJZ2_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4568383.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(10820..12259)
                     /gene="aldA"
                     /locus_tag="ACHJZ2_002290"
     CDS             complement(10820..12259)
                     /gene="aldA"
                     /locus_tag="ACHJZ2_002290"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO4568384.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(12456..13256)
                     /gene="ydcF"
                     /locus_tag="ACHJZ2_002291"
     CDS             complement(12456..13256)
                     /gene="ydcF"
                     /locus_tag="ACHJZ2_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO4568385.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(13372..13749)
                     /locus_tag="ACHJZ2_002292"
     CDS             complement(13372..13749)
                     /locus_tag="ACHJZ2_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568386.1"
                     /translation="MDERFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEH
                     YVIATSNDVIDVLSTVSPQIVYD"
     gene            complement(13874..14305)
                     /locus_tag="ACHJZ2_002293"
     CDS             complement(13874..14305)
                     /locus_tag="ACHJZ2_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568387.1"
                     /translation="MNVRNVKFDNLSFYEEKIRWRMRRQISNAEAIFYENQEEHIRRF
                     LGRYTCRKPVLVYWESEQRWTSVNGLEVMSMFGEHCHHIYLDDIKKKIQTELPENKTL
                     DFKRQLQFIILGDDKIRIWVPEGKLYFALMNVLQMFPMKNG"
     gene            complement(14292..14630)
                     /locus_tag="ACHJZ2_002294"
     CDS             complement(14292..14630)
                     /locus_tag="ACHJZ2_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568388.1"
                     /translation="MKNVENIIWHDGVLVETRMECHHTEYNFILTAELYVDEFTAGRE
                     RKRFEFTGVEHLSQVMDIAELIDNQNAGNIKDATIVYKAKKYKISLHLFGGFLSFTFK
                     TMRVIDNECA"
     gene            complement(14635..15060)
                     /locus_tag="ACHJZ2_002295"
     CDS             complement(14635..15060)
                     /locus_tag="ACHJZ2_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568389.1"
                     /translation="MSRHDILLRSQFERIIEGDRVGQALISFYEKLPEENYRRALYIL
                     SIIYPIKLNVGDDEFKFIFYIMSQKKFLRQQTISDFVRSINVIEFTETQKSVLRELIK
                     KNNDIIITQCTFELDCLLTRVSASSNQFRNSNGYLPENS"
     gene            complement(15206..19108)
                     /gene="hrpA"
                     /locus_tag="ACHJZ2_002296"
     CDS             complement(15206..19108)
                     /gene="hrpA"
                     /locus_tag="ACHJZ2_002296"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO4568390.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            19309..19914
                     /gene="azoR"
                     /locus_tag="ACHJZ2_002297"
     CDS             19309..19914
                     /gene="azoR"
                     /locus_tag="ACHJZ2_002297"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO4568391.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(19965..21257)
                     /gene="ynbD"
                     /locus_tag="ACHJZ2_002298"
     CDS             complement(19965..21257)
                     /gene="ynbD"
                     /locus_tag="ACHJZ2_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO4568392.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(21271..23028)
                     /gene="ynbC"
                     /locus_tag="ACHJZ2_002299"
     CDS             complement(21271..23028)
                     /gene="ynbC"
                     /locus_tag="ACHJZ2_002299"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO4568393.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYHRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(23044..23940)
                     /gene="ynbB"
                     /locus_tag="ACHJZ2_002300"
     CDS             complement(23044..23940)
                     /gene="ynbB"
                     /locus_tag="ACHJZ2_002300"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4568394.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(23940..24545)
                     /gene="ynbA"
                     /locus_tag="ACHJZ2_002301"
     CDS             complement(23940..24545)
                     /gene="ynbA"
                     /locus_tag="ACHJZ2_002301"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4568395.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(24716..27022)
                     /gene="ydbD"
                     /locus_tag="ACHJZ2_002302"
     CDS             complement(24716..27022)
                     /gene="ydbD"
                     /locus_tag="ACHJZ2_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO4568396.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(27086..27946)
                     /gene="pdxI"
                     /locus_tag="ACHJZ2_002303"
     CDS             complement(27086..27946)
                     /gene="pdxI"
                     /locus_tag="ACHJZ2_002303"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO4568397.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(28166..30625)
                     /locus_tag="ACHJZ2_002304"
     CDS             complement(28166..30625)
                     /locus_tag="ACHJZ2_002304"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO4568398.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(30845..33823)
                     /locus_tag="ACHJZ2_002305"
     CDS             complement(30845..33823)
                     /locus_tag="ACHJZ2_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HFO4568399.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVKHNPVVYKNG
                     VTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIVKSWTVHDDSNTVVFDGVNT
                     SGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAIITDQGGNTALNGGTVMTVDG
                     DNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVDGGTAVIINGDNTKLNTAGD
                     STITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVGDGATIKQEGDLYVSGGAHG
                     IDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMGDLNVGQAAAGENAIGIQID
                     GDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVGDFSTGLDVSGNNNNMTLAT
                     YELNVTGQKATGVNVSGDGNTIEIAGSILVDKDQKADNAQPYFFNPSTGVNISGDNND
                     VTLDGQLTVVADSKTTSRSYASYDGAQEHIAGIVIAGDDNTFTLNGGVHFVGEKNVMD
                     DGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFPLGFENLIQLSHGAELEIGA
                     DATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVELKNIGFAAAWNKDSTVINNG
                     SIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQHSVLNYPAEWNLSDGTSFN
                     NKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAIDKSTADNEGQITLDTLWVDE
                     NDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATATNHENGVITVYNAGAGMAAY
                     GNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGTAINDQTGVININAENGQAF
                     YNDGTGLIINYGTICTFGVC"
BASE COUNT         8761 a         8797 c         7681 g         8806 t
ORIGIN      
        1 atttgctacg gttatagtaa gtcacctgtg ggaaaacgct atgcgcaaat atcgtctaag
       61 tgaagaacag cgagccttta gttatcagga agatggcact aaaaaaaatg tgttattacg
      121 gcagattatc gccattagcg attttaacga tgtgatagca ggaaccgctg gcggctggat
      181 cgatcgcgaa acggtgttag cacaggaagg aaattgctgg atttacgacc agaatgctat
      241 tgcgttcggc ggaacggtga tttccggtaa tacgcgcatc accggcacca gcgtactgtg
      301 gggagaggtt tacgcaacgg ataatgtctg gatcgataat agtgaaatca accagggtgc
      361 atatataagt gacagtgtca ccattcatga ctcactggtt tgcgggcaat gtagaatatt
      421 tggtcacgcc ttaattgatc aacattctat gatcgtcgcc gcacaaggct taacgcccga
      481 ccatcaactc ctgctccaaa tttatgatcg cgcaagggtt agcgcctcgc gtattgtcca
      541 ccaggcacaa atttatggcg atgccgtcat ccggtatgct tttattgaac atcgcgccga
      601 agtttttgat tttgccagta ttgaaggtaa tgaagagaac aacgtctggc tatgcgattg
      661 cgctaaagtc tacggtcacg cgcaggtgaa agctggcata gaagaagatg ccattccaac
      721 gattcattac agttcgcagg tggcggaata tgccattgtg gaaggtaatt gtgtgttaaa
      781 acatcatgtc ttgattggcg gaaatgccgt ggtacgtggc ggaccgattc tgctcgacga
      841 gcacgttgta atacaaggtg agagccgtat tacaggtgcg gtgataattg aaaactatgt
      901 ggagttaacc gaccacgccg tcgttgaagc ctttgatggc gacacggttc atgtccgtgg
      961 tccaaaagtc attaacggcg aggagcgcat tacgcgaacg ccgctggcag ggttattatg
     1021 aatcgataat acgcgcgtat aagttcacat catcataggc atcattcagg aactcagcct
     1081 gtttcaggca accttcaagg ataaaaccat tgcgcaaagc gacctggttg ctttgcggat
     1141 tgtccacccg acatttgatc acgaagcgtc taagttcacc agactgagcg taatgatgaa
     1201 tcagtgcctg cagcgcctga gaaatgatcc cctgcccctg atgagattcg tccagtcagt
     1261 agccgatttc agcggtttta ttcagtggtt caatacgatt aaacgagata acgccgataa
     1321 gttcatcttc tttgaaaatc atgaacattt tggcatagcc gcgttgatgc aacatcacat
     1381 taccctgcac cgtttttcgc gtgtcctctt caatctgaac gaactgcggc cagtttagcg
     1441 actgctgtaa ccaggtttta tttttacaga ttaactgata aagaggagtg acgtgatttt
     1501 ctgcaacagc atgtaattca agtgattcgc ttacttttat cgtttcagtc atcttatctc
     1561 cagtcaatcc atgcagttgt taaaaaagcc gcctggctgg cggcttagga aagtttacac
     1621 ctctttgacg ctctcatgct gcaggatttt gagcatctca tcttttaact gtagcttttg
     1681 ttttttcatg cggaccactt ccgcattgta ccctcgatcg tcggaacctt cctttctggc
     1741 aatttcatga tcaagtttat tgtgtttatc gaacaaggac ataaagcgag gattttcgtt
     1801 tttcagacgg gatattaaat ctcgatattc tggaaacata cgcactccct gttagtggct
     1861 gacaaggtgt atctgaaata cacaccatca gattactcat ttaacttagg gaaaaatgcg
     1921 agcgggatcg tgtttaacca gacggcacct cccgaagaag gtgccgaaga aaaaacgatt
     1981 aactcatcac gcggtcgtca acatactgac gtttatccgg cgctggcggg aaatactgat
     2041 acagccatgt ttcactgata gcgtcgccct gacaacgcag atacatccgc atatcgaccg
     2101 gatcagtgga gtccgaagtc ggataccagt caaactgaat acgataacca tcaatgggtt
     2161 caatatagag aatttcgatt tgcttcgctt ccccactgga aagcgtaatc accggctcaa
     2221 tgccttttgg tgcggcagct ttcagatcac caccaacgaa atcgacggca aaacgacgcg
     2281 cccatttttc gggatagtgt tcacctggtg cccaaccttc cgggaaaccg cccatgccgg
     2341 tacgcgtcgc cataacgcgc gctaatgggc aatgaacagg cggttgcgca ctccagtaca
     2401 gacgatactg gaatgcaaac tcatcacccg cctttacagc tttttctggc tgccagaagc
     2461 agacaatgtt atccagcgtt tcgcccgttg ttgggatttc catcaggccg atggtgccct
     2521 taccccactt gttacgcggt tccacccaca gacttgggcg tttgttatac cagcccataa
     2581 tgtcctgata atgggagaaa tcacgatcca gttgcaataa accaaaccct ttcgggttgt
     2641 tgtcggtgta agcattgaac tgcaattttt gcggattatt cagcggacgg caaatccact
     2701 cgccgttgcc ccgccacatg gacagacgat cagagtcatg aatttgcgga tgaatggtgt
     2761 cgcacatccg acgttcatta gtaccgcagc tgaacatact ggtcatcggc gcaatgccca
     2821 gctgtttaat gtctttgcgc gcatacaggt gattttccac atccataatc acctgacttt
     2881 tctcacaatg gatagtgaac ttataggcac cagtaatgct ggcgctatcg agcaacgcat
     2941 aaacggtaaa ggtagttgcc cccggtttta ccgtatcaaa ccagaaggcg gtaaagtcgg
     3001 ggaactcttc tttactgtcg gtgtaagtgt caatcgccag gccgcgggcc gacaaaccgt
     3061 attgatatgt gtcatcaacg gcgcggaaat aactcgctcc gaggaatgat actacatcac
     3121 ggcgcgccag ttcgggggct ttaaacacgc gaaaaccggc aaagccgaga tcgctttgcc
     3181 cttctaattg tttggtatca acacctgcat cgttgtattt gaacaactcc gggcgaaagt
     3241 gaatttcacg cgccagatgt gttgctggat ctacagaaaa catacgaacg cggcgacgga
     3301 atcccattcc catatggaag aactgagcgt ccagttgacg gttctcaacg ttatgccaga
     3361 gcgatttttc ggcgtcgtat tgaatactgt tataagcctg cggcgtcatt gtcgccagcg
     3421 tgtcaggtaa cggacgaggc gcaccacgcc acgctgtttg cgctaagtcg tgcgccattg
     3481 actgtagaat ggagaagtca aaacgctggg tttgcccgtc ggcaatatca gaatctgccg
     3541 cgaatgccgc ctgagaaaaa agagaagcaa tgccgctggt accgcacacg gcggccatag
     3601 ccattgaacc tttaataaat cgtctacgat ccatacctga aagtgagtcc ttctggtgtt
     3661 gtgaatggtc ccgcgctggt cgttttcagc ggcaagaaag tgcttattta tgaccgctca
     3721 ctctagacaa tatttattaa taatccaatt gttgaactga gagaatatgc ataaatcggc
     3781 aattttttta tgtcgatggg atgagcctga ttcgtttgta aaatcaggca aatttttgca
     3841 aaggcggatg gtcagaattt aaagcaaacc acagcgacgt ttactgcgtt cttccgcttc
     3901 ctgataaagc tggaactcat ccaaaaccgg acaacccaac gcctgctcaa atgcttcgcg
     3961 actggcatta ccgtggctgc gtgcctgcgt ttcattaccc agatttgact ggaagatccc
     4021 cgccgcgcta acgggcaaga aatcttcata ggtaatgggt tgcgccgcga cccaaccacg
     4081 ttcaattaag ggctgtggat cgtctccggg atgaatcgcc tgacgatgcg cctcacctga
     4141 aggcgtcaga cggtaccgga accatgccag cccttgctga cgcattaaaa actcactgtc
     4201 aggaaaagtg cggaaggttt cctgtaaatg catttggtga gtgagattat cctgcccggt
     4261 tccagcgtta cgcagaagat catcatacag ttgtcgacct ttcggcgtta atgccacgcc
     4321 acgctgctca atttcaccaa agcgcgcagt atgcgtgccc tgtttctgcc ccgcaaacaa
     4381 caccgtctct tccagtgctt taaagctggt ctggcgtagt aaaatcggta cctcgcggcg
     4441 cggcggccct tcgatcagaa tttttggttc aattccgcat tcaggcatca tcgactgcac
     4501 ccggtcaata tccagcgtac gtggcgtcag atggttgata tggcatccag gaaaacagac
     4561 cacatcagca atcaaccgat gttcgttgtg caaggcgcga taggtttctt catctaccgt
     4621 tgctgactgg tgccaacgga acgtttccag ggcctcctgc acaaactcct gtgcctgtgt
     4681 ttcgttaaaa ccgccccgct ggtcatactc ctctaacagt tgtcgacaac gtggggtgaa
     4741 gatatcgcgc tgacgcagaa tctccgccgc tttctggcgc aaaatttcgt tctcgataag
     4801 ctcaaggcgg agtaaggagg taaaaacgcg gaagggatta cgcgccagag aagcatcatc
     4861 aatgggccga aatgctgtcg aatgtaccgg cacccctgcc tgcgagagat cgtaatagct
     4921 caccgggtac atccccataa tggcaaacat ccgccgaaga gtagcaagct cttgtgcagt
     4981 cccaacgcga atcgccccat gacgttcaac attcagtcgc gccagctcgt ctgcatttac
     5041 cattttttcg tgcagttgag gattgttttc cagcacagcc agattcacat cagctaccag
     5101 ttccagcagc gtgccatatt gcggaacttc ttgctggtac atggctgaca ttgcctgcaa
     5161 aaactgttcc cgaatctcat ccgccgtgat gctgttcgcc atagtgtctg cctccagtgg
     5221 tcagtaatct ggagtgtagg taaccgcatt cactctggcg ggaagaattt acaaactgtg
     5281 atctcgccgc gaaaacatca atattatcca ttttgctgta acataattgc tttaattgtt
     5341 aataatattt tgcaatcaag ttatcataat caaacaactt cacttgtcag cgacaccgct
     5401 tcgtttttaa catcgcttat ggaaaaaaat agtctgttta gtcagcgcat ccgtttgcgc
     5461 caccttcata cattcgtagc tgtcgcacaa caaggaactt tggggcgcgc ggctgaaacc
     5521 cttaatttga gtcaacctgc gctctctaag acattaaatg aactggagca gctgacgggc
     5581 gctcgcttgt ttgagcgtgg tcgtcagggg gcgcaactta ccttacccgg cgaacaattt
     5641 ttaacgcatg cagtcagagt acttgacgcc atcaacactg ccggacagtc gcttcatcgt
     5701 aaagaaggtc ctaataatga tgtcgtcagg gttggtgcac tacctactgc ggcactgggg
     5761 atattacctt cggttatagg tcagtttcat cagcaacaaa aagagacgac cttgcaagtt
     5821 gcgacaatga gtaaccctat gattctggcg ggtttgaaaa ccggggaaat cgatatcggc
     5881 attggtcgga tgtcagatcc tgaactgatg accgggctta attacgaact gctgtttctt
     5941 gaatcgctga agctggttgt ccgccctaat catccgctac ttcaggagaa cgtaacgcta
     6001 agccgggtgc tggaatggcc ggtcgttgta tcaccagaag gcactgcgcc acgccagcat
     6061 tcagatgcat tagtacagag ccagggatgt aaaattcctt cgggttgtat cgaaacgctg
     6121 tctgcttcgc tatctcgtca acttacggtt gaatacgact acgtgtggtt tgtcccttct
     6181 ggcgcggtaa aagacgacct gcgtcatgcc acgctggtgg ccctgcctgt tccgggacat
     6241 ggtgcaggcg aaccgattgg aatactgacc cgcgtagatg cgacgttctc ttctggttgc
     6301 cagttgatga ttaacgctat tcgaaaatca atgccgttct gaaaggtgaa gggatctgtc
     6361 gatccctcct ttaacatttt cacaccggag cgaaactaac tggttcaccc gctccgcgag
     6421 gttctgccaa cacagattgt ttgttcagac ggaatacgtc caccgcctca gttaatcgtg
     6481 ccgcctgttc ttcaagggac accgccgccg ctgaggcctc ttctaccaga gaagcattct
     6541 gttgcgtcac cttatccatt tcagaaatcg cctggctaac ctgcgttatg cccctacttt
     6601 gctcatccga ggcggcggca atttcctgca tgatatgtgt gacactcgcg acggcatcaa
     6661 caatagtgct catggttttc ccggccgttg ccacctcatc cgatcccagg tcaattaacc
     6721 tgactgattc actgatcaag ccttcaattt cttttgccgc ctgggcgctg cggcttgcga
     6781 gtgtccgtac ttcgctggcg acaacggcaa atccacgccc ttgctcaccc gctcgcgcag
     6841 cttcaacggc agcattcagt gccagaatat tcgtctggaa agcaatactg ttgatgacgg
     6901 cggtgatctc agaaattttc ttcgaactcg tggagatagc gcccatcgtt tttactacac
     6961 cggaaaccgt ctgcccgcca tcgctggctt taatagaagc ctcttgcgcc agtttgctgg
     7021 cataatgcgc gttatccgca ttctgtttca ccgtcgcagt gagttgctcc atgctggcgg
     7081 cagtttgttc gatagccgcc gcttgttctt cggtgcgaga tgacaggtcc gcattgccag
     7141 ctgaaatttc gctggtgcca cgataaatct cttccgcacc ctgtcgaaca gtccctacgg
     7201 tcatccccag tgaatgctgc atttgctgta aatgacgact taagcgaccg atttcattac
     7261 gacccgccgg ttcatccttc atcgtcagat cgccactagc gattttttca atccgttgtg
     7321 cggcatgttg cagtggacga atgacgatcc gacgtagcac cataaatgtt atcagcgtca
     7381 tgaccagggc aagcacaaac gcaccaatca tgaacatccc gcccaggcgg gtgcgctgat
     7441 gggccagttc cgccagttgg ttggctctgg tgctacgtat cttaacggct ttgttcaaaa
     7501 tatcggtata agcattatcc agcgttcgga tctgctcact ttcatgattg ataatcgctt
     7561 caaacatgcc atttttggca tatttcaaca taggttgcat acccgtgata taagcctgaa
     7621 agcgttgatt taattcagtg tcgagggctt catcagcagg tgttttcacc ggccgattct
     7681 gataagcacg ataaccttgc tgcgactgtt taatctccga ttcggcttgc gcaatatttc
     7741 gcttcattgc ttccatttcc gcaatacgac tcgcagcccc ggcctgaatc atattgatcc
     7801 gggctgaacg caaaaaatct gaactgttcg ctaacccggt ccgaatctca atctccgcag
     7861 taacatcacg ctgatcgcga tcggcctgcc agaggaaata gccagccagg gctgaactta
     7921 atgcgaatag aactaagata ccgcctagaa tgcaggcaaa taacggaacc aacctgatgt
     7981 gatgcaaaaa acctaatcgc tgtgagggag ttgtattcat taccgttgtc tctcgtccag
     8041 gtttactcct gatagaaagt catcggcatt tgacggtaat tacttacgac taaaagcctc
     8101 tttgcgtcac atttttacaa caattattgc agagaagatt aatgccattg gaatgaagag
     8161 agtaacgaat ggttaaactg gctaacccga atgacgaaaa attctggaaa acagcacgca
     8221 atttcgtcag gtgattattt tcgtccctaa ggaaaccaca taggatgctc acgggtgcct
     8281 gagactttct gttttcaggc taaccgggaa tcagaaagac gaaggccccg accgatattt
     8341 ctatcaaccg aggccaacga atgaaccaag caattcagat gttagcctct tacccgccat
     8401 ccggcaagga gaaaggctat gaagcgcaac cctctggtgg tgtgtctgct cattatctgc
     8461 attacgattc tgacattcac actcctgacc cgacaaacgc tctacgaact gcggttccgg
     8521 gacagtgata aggaggttgc tgcgctcatg gcctgcacgt ccaggtaagg gcaagcgcgg
     8581 ggattttccc cgcgcatctg actagcagat tcattttctc atggcacccc ttttacactt
     8641 cagttagtgc atgttttttt cgatgcacta atagaaccat cattacagac aaatttgccg
     8701 cctgccgtac agtgtgagat cccccctttc gaaccagaac agggtttgcg cccggcatcg
     8761 gcataaaagg aaacaagcgc tcccataaac aaaataagtg ccagtttttt catataacct
     8821 ccatggttta tcgaatctta tgttcgattt gaacgttata cgctggttta ttttgatacc
     8881 gttgatgaga tcaaagatcg ggtgaattga ttaaaaataa gccagatgga cgtatgggca
     8941 aggattatgg ttttatttgt catacaaata aatataatag gcgcttccga tgtagacccg
     9001 tattcttcgc ctgtaccacg ggtcggtttt agtacaggcg ttttctttaa atatccttca
     9061 ggaacgttta cgcggcatca tgcgtagaag tgtgttgtcc ttccagaaat agtggtgtgc
     9121 cagtgcagcc gcagcgtgca aaccgatgac aaaatatccc agattcgcca gcgtttcatg
     9181 ccacgacttt aagctatcta cccgttcgaa attggcctct gaagcgtaag gcatcgtcaa
     9241 accaaacgca aaccacgggt tgcctcggtt atacatcatc accaaaccaa tcactggcag
     9301 cgcaataaac aggagataaa tcaccaaatg tcccaaatgc gccagtcccg tcatcatcgg
     9361 ttttggctta ggtataatcg gcggggttgg gtatttcagc cttaacaaaa gacgaacgac
     9421 catcagcacg agaattgata tgccgcagga aacatgaatc atgttgatga gtggccgatc
     9481 gctacgtggg aagaaacccc gaaactccat tgcgcaatac gctgcgataa ccagcaaaaa
     9541 gaccagccag tgaatgctga tttgtaacct tgagtattta ttttccataa catttcctgc
     9601 tttaacataa ttttccgtta acataacggg cttttctcaa aatttcatta aatattgttc
     9661 accctttttc agtacgttac gtttcactgt aagaatggag attgcccatc ccgccatcct
     9721 ggtctaagcc tggaaaggat caattttcat ccgaacgttc ctgacaggag aaaaccatga
     9781 gtaaagttgg tattaacggt tttggtcgta tcggtcgact ggtgttgcgt cgattacttg
     9841 aagtcaaaag caacatagac gttgtcgcta ttaatgatct tacttcccca aaaattctcg
     9901 cctatctgct gaaacatgat tcaaactatg gtccgttccc ctggagcgtt gattttacgg
     9961 aagattcact tatcgttgat gggaaaagta tcgcggttta cgccgaaaaa gaggctaaaa
    10021 atattccgtg gaaagcgaaa ggtgcagaaa tcattgtcga atgtactggc ttttatacct
    10081 ccgccgagaa atcgcaggcg catcttgatg ctggcgcgaa gaaggtgttg atttccgccc
    10141 ctgcaggtga aatgaaaact atcgtttata aagtcaatga cgacactctg gatggcaacg
    10201 acaccattgt ttccgtggca tcatgcacta ctaactgtct tgcgccgatg gccaaagcct
    10261 tgcatgacag tttcgggata gaagtcggca cgatgacgac cattcatgcc tatactggca
    10321 cccagtcact ggtggatggc ccgcgtggta aagatttacg tgcttcacgc gccgcggcag
    10381 aaaatatcat tccccacact acgggcgcgg caaaagccat tggtctggtg atcccggaac
    10441 tgagcggcaa actgaaaggt catgcgcaac gcgtgccggt gaaaacaggt tcggtcactg
    10501 aactggtatc gattctcgga aaaaaagtga ctgccgaaga ggtgaataac gcacttaaac
    10561 aagcaaccac caataacgag tcatttggtt ataccgatga agaaatagtc tcttccgata
    10621 tcattggcag ccatttcggt tcggtgtttg atgccacgca aacggaaatt accgccgtgg
    10681 gcgatttaca actggtgaaa acggtcgcct ggtacgacaa cgaatatggc ttcgtcacgc
    10741 agcttattcg caccctcgaa aaattcgcta aactctgacg cgcacaggcg gaggaaaaaa
    10801 cctccgcctc tttcactcat taagactgta aataaaccac ctgggtctgc agatattcat
    10861 gcaggccatg tttaccatct gcgccgccaa taccggattt acgccatccg gcgtggaagc
    10921 cttgcatagc ttcgaagttt tcacggttga tgtaagtttc accaaacttc agccctttaa
    10981 tggctttcat cgcgacgttc agattttggg tatagattga tgaggtcagg ccgtaatcac
    11041 tgtcattagc cattgagata gcatcttcca gcgtgtcaaa tgcgactacc ggcagaaccg
    11101 ggccaaaggt ttcctcatgc ataatcgaca tttcctggcg aacatccagc agcaatgtcg
    11161 gcggataata atatcctttc ccctctaccg ctttgccacc caacgccact ctcgcccctt
    11221 cttctactgc gcgcgccact ttttgctcga ccctttccag cgccgcggcg ttaatcaacg
    11281 gccccatcgc aatgtcgttg cgttcagcgg ggttaccaaa ttgaaccgcc tgcatcgctt
    11341 cacccagccg attgacgaac tgatcataaa tgcctttctg tacataaaca cgttctgcac
    11401 agttacacac ttgcccacta ttaatgacgc gtgaatcaac gatggctttg actgccagtt
    11461 caagatcggc atcgtccatt acgatagctg gtgctttacc ccccagttcc aggcacactt
    11521 tggtgatgtt tttcgccgca gtcgccatga tcttctcacc tgcagagacg ctgcctgtca
    11581 tactgaccat tgcgaccttt gggttacccg ccagttcttg cccaacggtt tcaccacgcc
    11641 ccagaacaag gttaaacacg ccgcgcggaa ggcctatttc atcgacgatt ttggcgaatg
    11701 caatcgcatt gtttggcgta aattcactgg gtttaatgac aatggtatta ccagtcaaaa
    11761 gagcgggagc cattttgcgg gcaatgagga agaacgggaa gttccacggc agaatgccgg
    11821 tagtcacacc aagcgcacgt ttaaacagaa gaatattttc tcctggacga tcgctttgaa
    11881 taatctcgcc ctcgtaacgc cgtgcccact ccgccatgta atcgatatag tcggcagtaa
    11941 aagccacttc gacttcagcc agctgctgga tcttgccccc ttcttcaaca atcagcgcac
    12001 tgatttcact ggcgcgttcg cggatcccgg cggagatttt gcgcaaccaa ctggcgcgtt
    12061 caattgcagg caacgcttcc cattctggtt gtgcacgttc tgctgcatcg attgccttac
    12121 gggcatcctc ggcctgacca tcgggaatgc gggaaatgac agcctctgta gcagggttta
    12181 ccacatcaat ccatgcgtct ccacgccagg taacaaactg tccatcaata tacataggat
    12241 gttgaacggg tactgacatg ggcgactcct gtgatttata tgtttgtttt tggtaaatac
    12301 attgttaatt aacatcaatc atcatagaga cggcatcatg aaaggacaat tctgtgaagg
    12361 gcttcataaa aacaagtgta aaaggaataa gaggcagaaa taaaaggcag gagttatgcc
    12421 cgtttgcaaa caagtggttc acaaacgggc aatttttaac gtaacgagcg actttccatc
    12481 gcctcgatga gcaccgcatc gtgtttcagc gtttgccatg catggatgac ttctgccgga
    12541 aaatcaacgt gaacgataaa atctcgcccg cggggaccgt agccatcgct atcatcgcgt
    12601 aaacgcggca gctcgccagt gagtagtgag agataacgct caaccggcca taatccttgt
    12661 aactgattaa taaagattac actgtctgcg ttatttccta actgaggaac gaatccggga
    12721 taacttaacc agcgtggtac atcgggattg tccccggtca tacggcggaa cgtcgccatc
    12781 gtgcgccgct gcatggtggg gtcctgtaca acgatagctg tgtgaactcg ttctacggcc
    12841 tgattcaata gcgcgatgct aaagcgtgcg ttttcaccgc agtttgttga ctggtcttca
    12901 atccagattt tttcatgcgg aatatgccag aactgatgag cgatatccgc caggatggtc
    12961 gcttctgctc tgccagtggt gcggatagtg ttgtagtgcg gatgctgtgc gatggcgcta
    13021 tacaaaaaag tggtcgagtg accgatacca ccactaatca gtaaaggaat ttgctgatcg
    13081 cgagcaatct tacatgccgc atcgatagtc ggcataaccg catttcctgc aagtatgacg
    13141 caatcagcct gatacggcac ctcaccggag aaatcatcct gcgccagcca ctgtccgata
    13201 acatttatgg catctatagt tgccgtcgaa agcgtcggaa acggggtaat gttcatggtt
    13261 cctccttatc gttcccttaa gaatactgcg tgagataata gtgagaagcg gagcactctg
    13321 aaaggaagat aatgcttttt ctatctcgcc atcgcgaacc agacactggt tttagtcata
    13381 cactatttgc ggagaaactg ttgataaaac atcaataaca tcatttgacg tggcaataac
    13441 ataatgttcg atcattttat ttttatgaat ttcacaactt tgcatagtaa accatgtaag
    13501 ataagtggaa ttggctactc tatagatccc cgtttggtta attccctgct ttcctgacat
    13561 acctaacaaa tcaccttcgt cagtaacacg aaatgagtgt atccagtcaa atgatatctt
    13621 aacttctgac ttgatcgccg ggcatatcag ggccagttca agcctggcat tcttatagct
    13681 taaacctgac agatcggcgt gtttaaaaga caacgaagtc ggcgtgatat ttataaaacg
    13741 ttcatccatg ttaacaccct taatatatga gactgtactc aatttaacct tctacaaaaa
    13801 tggtgtgagt ctgtttaagt accatgtata atcgataacc gttgctgaat tttaaaatta
    13861 tcttgtccag ttattaacca tttttcatgg gaaacatctg caatacattc attaatgcga
    13921 aatacaattt cccctcaggc acccatattc taatcttatc atcccctaat attataaatt
    13981 gcaattgacg tttgaaatct aaagttttat tttcaggtaa ttcagtttgt atttttttct
    14041 tgatatcatc aaggtagatg tgatgacaat gttcaccaaa catactcatc acttccagac
    14101 cgtttacaga tgtccatctt tgttcacttt cccaataaac aagaaccggt ttgcgacagg
    14161 tatatctgcc taaaaagcgc cgaatgtgtt cttcctgatt ctcgtaaaat attgcctctg
    14221 cattcgagat ttgccgacgc attcgccagc ggattttttc ttcataaaaa gacaagttgt
    14281 cgaatttgac attacgcaca ttcattgtca ataactcgca tggttttaaa tgtaaaactt
    14341 aaaaaaccac caaacaaatg aagtgaaatt ttatacttct ttgccttata aacaattgtc
    14401 gcatctttaa tattacctgc attttggtta tctataagtt cagcaatatc cataacctgc
    14461 gataaatgtt ccactcctgt aaactcgaac ctttttcgtt cccttccggc tgtaaattca
    14521 tcaacataaa gctccgccgt caatataaaa ttatactcgg tgtggtgaca ttccattctg
    14581 gtttctacta aaacaccatc atgccagata atattttcta catttttcat gctattaact
    14641 attctcaggt aagtaaccat tagagtttct aaactggttc gatgacgcag atactctcgt
    14701 taacaaacaa tctagctcaa atgtgcattg cgtaattatg atatcattat tctttttaat
    14761 aagttcacgc aaaacacttt tttgagtttc agtaaattca ataacattta ttgagcgtac
    14821 aaaatcgcta atggtctgtt ggcgtagaaa ttttttctgt gacatgatgt aaaaaataaa
    14881 cttaaattcg tcgtcaccga cgttaagttt aatcggataa attattgata aaatatacag
    14941 cgcgcgtcgg taattttcct ctggaagttt ttcatagaaa gatattaaag cctgaccaac
    15001 gcgatcaccc tctattatcc gttcaaattg cgacctcagt aaaatatcat gtctactcat
    15061 tgcaatacgt cctgttcgcc caaggcgaag tatgaatgaa acttatgtaa aatagtaccg
    15121 cccttgctat atttcagaat tcataaagaa aaaacccggc gctaaatgct taccgggttt
    15181 tagatttatc aggcaaatag cagggttaac cgctaatctg ctccatcgcc tgcaaaatac
    15241 gcttatctga aatcggataa ggcgtaccaa gttgttgagc gaagtaacta acgcgcagct
    15301 cttctatcat ccaacggatc tctttcacgt cttcatcctc acgacgtgca ggcgggagtt
    15361 tgttgaacca ttgctgccac gcctgctgga cgttttcgac tttcagcatc tgtgcacgat
    15421 cgcgatgtgg atcaaccgcc agtttttcca gccgtttttc aatcgcctgc aaataacgca
    15481 gcgtgtcgcc cagccgtttg aagccgttac cagtgacaaa accgcgatat accaacccgc
    15541 ccatctgcgc tttaatgtca gaaagcccca gcgccatggt catatccacc cgccctttca
    15601 gacgtttgtt gatattgaac actgccgtaa ggatttgctc gacctgcttc gcaatatcca
    15661 ccaccgtgtc gttcagttcg gcacgcactt tttcatgcag cgcagcaaag ccttcttccg
    15721 tccagaccgg gcctccattg gcgtcgatca gttgatccac accgcatgag atacagtcgt
    15781 cgatcagctc cagcactttg ccatacgggt taaagtacag ccccagcttg gctttgtttg
    15841 gtaacttctc atgtaaatac ttgattggtg agggaatatt cagcagcagt agacggcgaa
    15901 gaccgttcca cattgcctgt ttttgctcca gcgggttatc aaacagtttg atcgccacac
    15961 tgtcgcgctc atccaccagc gccggccacg ccttcacttt gtagttacca cgcttctgtt
    16021 cgtagctttc cggcaactga ccaaaactcc agatatgtaa gccgctctgc tcgataccgt
    16081 catccgccac cgcagatagc gtttcctgca ctttgccttt cagcgcatct ttcagatctt
    16141 gtagcgagcg cccttctttt agcttcttgt ttttgtcatc caccacgcgg aaggtgattt
    16201 tcaggtgatc gggcacctga tcccagtgcc agtcttcgcg gtcaacggta acgccggtca
    16261 tccgccgtaa ctcgcgctca aggctgtcga gcaacggtaa ctccagcggt gtgacgcggc
    16321 ctaaaaacgc ttcggcatag tttggcgcag gtacaaaatt acggcgtacc ggtttcggca
    16381 acgatttaat cagagcaatc accagttcgc ggcgcagacc agggatctgc cattcaaacc
    16441 cgctttcctc aacctggtta agtaacggca gcggaatatg tacggtcaca ccgtcagcat
    16501 ccgcgccggg ctcaaactga tagctcaaac gcagcttgag attgccctga tgccagaagt
    16561 tcgggtaatc cagcttgctg atcttttccg ccccttcttt gatcaacatg ctcttttcaa
    16621 agttgagcaa atcaggcgtt tcgcggctga cttttttcca ccagctgtcg aagtgacgag
    16681 cggagattac atcgtggccg atgcgctggt cgtagaactc aaacagcgtt tcgtcatcaa
    16741 ccagaatatc gcggcgacgt gatttgtgtt caagctcttc cacttcggcc cgcagtttca
    16801 ggttttcacg gaagaatgcg tgacgcgtct gccagtcacc ttccaccagc gcgtggcgaa
    16861 taaagagttc acgacataac gccggatcga tctggctgta gttgaccttg cgcgcggcaa
    16921 caatcggcaa accataaaca gtgacttttt ccgttgccat caccgcgccc tgcgcccgtt
    16981 cccagtgcgg ttcgctgtag gtacgtttaa tcaaatgctg agcgacaggc tccacccatt
    17041 ccgggtcgat acgcgcagca atgcgccccc acaggcggct ggtttctacc agttccgcca
    17101 ccattaccca tttcggcggt tttttgaata aaccagaacc ggggaagatg gagaaacgcg
    17161 cgttacgtgc gccggtatat tcttgtttat cggcatcttt catgccgata tgggaaagca
    17221 accccgtcag caacgcaatg tgaatttcac gatactccgc cggttcgctg ttcaccggaa
    17281 tgccgagttc tttcaccacc tggcgcaact gggtgtagat atcctgccat tcgcgcacac
    17341 gcagatagtt gagataatcg gtacgacaca ggcgacggaa ggcgttggaa gaaagcgcct
    17401 tttgctgctc gccaagataa ttccacagat tcacaaacgc gaggaagtca gactctttgt
    17461 cgtggaagcg acgatgtttt tcgtccgatg cctgctgttt gtccatcgga cgttcgcgcg
    17521 gatcctgaat ggagagcgca gacgtgataa tcatcgcctc acgcacgcag ccatgttttt
    17581 gcgcttccag caccatacgc gccagacgtg ggtcgacagg caactgcgag agctggcgac
    17641 cgagcggcgt cagtttatag gcgctggcct gttcatccgt ggtgatcgcg cccagttctt
    17701 cgagcagacg cacgccatcc tggatattgc gtttatccgg tgcttcgaca aacgggaacg
    17761 cagcgatatc gcctagcccc agcgcggtca tctgcaaaat aaccgaggcc aggttggtac
    17821 gcagaatctc cggatcggta aactccgggc gcgagaggaa atcgtcttcg gaatagagac
    17881 gaatacagat cccttcggac acacgaccac agcggccttt acgctgattg gcagacgcct
    17941 gcgaaatggg ctcaatcggc aaacgctgca ctttggtgcg atagctgtag cggctgatac
    18001 gcgccgtacc ggggtcgata acgtatttaa tccccggtac agtcagcgag gtttccgcga
    18061 cgttggtcgc cagcacaatg cgtcgcccgc tgtgcgactg gaacacgcgg ttctgttcgc
    18121 tattcgaaag ccgcgcataa agcggcaaga tttcggtatg gcgtaagttc agcttgttca
    18181 gcgcatcggc ggtatcgcga atttcccgct cgccgctcat aaagatcaga atgtcgccgg
    18241 ggctttcctg gcttagttcg tccacggcat cgaaaattgc ctgcaactgg tcgcgctcag
    18301 tgtcgtcggc ttcttcaaca atcgggcgat agcgcacttc caccggatag gtccgaccgg
    18361 agacttcaat aatcggcgca ttattaaagt ggcgcgaaaa gcgttccggg tcgatagtcg
    18421 cggaagtgat aatgattttt aggtcaggac gccgcggcag caactctttc aaataaccga
    18481 gcagaaaatc gatattcagg ctacgttcgt gcgcttcgtc aataatgata gtgtcgtact
    18541 gcatcagcag gcggtcttgc tggatctccg ccagcaggat accgtcggtc attagcttga
    18601 ccatcgtgtt atcacttacg tgatcgctga aacgcacttt gtaaccgatg caaccgcccg
    18661 gctccgtttt cagctcttcc gcaatacggt tcgctaccgt tcttgccgcc aggcgacgcg
    18721 gctgggtatg gccgatcagc cctttaatcc cgcgccccag ctccatacag attttcggta
    18781 actgagtcgt tttaccagaa cccgtttccc cggcgactat caccacctgg tgatcacgaa
    18841 tcgcttcgag aatgtcctgt tttttctgac taaccggcaa attgtcagga taagtaattt
    18901 ccggtcgtgc cgcttcacgc agcaggactt tccctgccgc ctggtcaatc tctttcgcca
    18961 tctcctggaa aatggcctgt tgtgcatcag gatttttaac cttcttcacg ccgtgcagac
    19021 ggcgagaaaa acgcagtctg tcacgcagca tcagcgaatc cagccgctgc tgcaaggccg
    19081 taaaagtcaa tttttgttgt tctgtcataa cgttagtggg cagtagtctg cctgattaat
    19141 tttctttgaa atgatagata tagagtacca catcgcggct ttctatgcgt tgttcaataa
    19201 attcgaacat aggcttcgat ttattgcgct atctctgtgg caggattgtg aataaagtgt
    19261 caacaagcaa cggggcatcg cccattcaaa tatctataag gaaacaccat gagcaaggta
    19321 ttagttctta aatccagcat cctggcaggg tactctcagt ctaatcagtt gtccgattat
    19381 tttgttgaac aatggcgcga aaagcactcc gctgatgaaa tcaccgttcg cgacctggct
    19441 gcaaatccaa ttccggtact ggatggcgaa ctggttggcg ctctgcgtcc gagcgatgcg
    19501 ccgctgacta cgcgtcagca ggaagctctg gcactttccg atgagctgat tgccgagctg
    19561 aaagcccatg acgttatcgt tattgcggca ccgatgtata acttcaacat ctcgactcag
    19621 ctgaaaaact attttgacct ggttgcacgt gcaggcgtta ctttccgcta taccgagaat
    19681 ggtccggaag gtctggtaac gggtaaaaaa gccatcgtta ttaccagccg cggtgggatc
    19741 cacaaagatg gaccaacgga cctggtgacg ccgtatctgt ccacgttcct gggctttatc
    19801 ggcattaccg atgtgaaatt tgtcttcgcc gaagggatcg catacggtcc ggaaatggca
    19861 gcgaaagcgc agtctgacgc gaaagcagcc atcgacagta ttgttgctgc ataaccggac
    19921 acgatcttga tgtggactgt agttcagtcc acatctcaat ccacttacct gttttcccat
    19981 aatttcaaca tcgctttgtg ctcttcctta agcacaatat gcgagcgtct ggctcgaata
    20041 aagctaatcg cttcatcaac tgttttacag tgtccgtaac ataacagcca tgccgccact
    20101 accagcgcac tgcgcgataa tcccaatgcg caatggacca gaacgctgcc ttgctcttcg
    20161 cgtaatgttt ccagcatcgc cacggcctgt cggagctccc cctcttccgg aaccaccaga
    20221 tccagcatcg gtacacagaa atagagtcga tcttttgtcg ctcgtccgcg agggaattca
    20281 aaggtgacat ccagaaccgc attctgtgcc ggaatatgtc gtggaaacgc ccctaaataa
    20341 acaccagcag tcattttgct caccggctcc aggcgacgac aaaaccaacg catagatagc
    20401 cacatcccga tgcgccaggg caatgtcagc cagtaaacgg cgggcgggag tttcccctga
    20461 ctatctttcc ctgttgttat cgcgccaagc ccaccgtacc cacggccaat aatgagtagc
    20521 gataatactg gccaacataa ccagactgac caccataact gaaccatcat cattagctcc
    20581 atcaacacaa tgcacgcgca cgcgcctacg acatatggca gtgctatttt gattcgacgt
    20641 tgatcaggtt tctgataatt ccaacgacgg tcgacgggta tcatccagtc aatcaacata
    20701 cctaccgcca gccctgtgat gacatcaata aaatgatgct gccaggttgt cagcatcgaa
    20761 atggcgatga gtaaaaacca tccgccgcag actttacgcc acctcacagc cagatgctga
    20821 cgaaagtgac gccaaagtag ccagcagaga acaatatgca gcgaaggaga ctggttataa
    20881 ggcagatcaa acagttcaag ttgcgaaaat agccatcctg tcaccccact cacttcagga
    20941 cggataaaac taaacttcag cgggtagaga aaaaaaccgc agcaggccat taccgttgcc
    21001 agaataagcc ggtggacaag tcggcgctgt tcgaatgtcg agctacaaac gaacagcgaa
    21061 aatccatata aaagatccag actccagtaa gggacaatag tccagggaag aaaagggatc
    21121 gccgtttccc agccgaatac ctgactgggg atatcatggc tgttaaagtc ctgaaccgcg
    21181 gtgaactgat ttagagatcc ataggtgaag aagaaaaacg gggccagcaa caataaccag
    21241 ccagcgcctt gtagcaatac gttccggcgt tcagttatca cgacgcaccg ccatcgaaac
    21301 cgtaaaaatg ccccactcat caatccgttg tgtgcattta tcaaatccgg catcacgcac
    21361 gagtgaatcc atctcccctt gcgaacgcac gcgcattacc cacggtttac catctttatg
    21421 actggttaac accccggcaa tcagctccag ttgtgggtgc cacggctgcc cggtgtagag
    21481 gagaatgccg cccggttcga tggcattggc aagacctgcg agtgagtttt ttacctgctc
    21541 atttccggga aaaagctcat acagtccaga gacaatcgcc agcgtaggcc gcggagttaa
    21601 cgcgctgagt tcctccgggt taaacgcatc gccctgttca aaacgcaccc gcccagacat
    21661 tccccgttga gcaatcatct cttgcccctg tgcaacattt aactcgctgt aatcacgtaa
    21721 caaaatatcg cttacggcag gctcgtttgc cagcgcatcc agtacatagc gcccatgccc
    21781 tgcggcaatg tcaaccacgc ggacggctaa acctttggcg tggagatcgg caacggcttg
    21841 tttaatcagt atttgtaaat gggttttgcg ctggcgaata ccgcgccagc caacactgtt
    21901 caggtagatt ttgtcgatta agcgcccgaa tgcgttacta ccttgcggtt gattttgata
    21961 gacataatcc agcgaactgc ctgaatcaaa gccggtgctc attccgagat gcaggcccgc
    22021 agagtgcgtc ccgaacaatt tcatcgcttt acgcataaag cgatacgcca aatcaaccgg
    22081 cgataatggc acgggtccac ctgaaaggag ccgccagcga tccgctgatg gtccagtgcg
    22141 gtcttcatgc tgataatcaa atttttgtga cttgttagca tataaacgac taataaagct
    22201 ttgcattttt tcaaatgcct gcgccctgtt ctcttcaccc aacgtgtcgt gataaaagcc
    22261 tggcagcaga tgcagctctt tcagagggct acgtaatcta tgataaaaat caatctgcgg
    22321 ttggcgatgc accacatagt catcgcctga tatcagaagt tgcgtgggga gcgtaatcgc
    22381 cgccgcatcg ctaacaatac gttcagacgt tttataaaga tcgagcaaga tattaacggc
    22441 aatcgcccgt gtgatcagcg gatcattatt gaaactcgcc acccgttgcc gatcgtgggt
    22501 caaatagcgt cctttcacat aggaattaat aaaaaacaga ccacgcagac gatgccataa
    22561 cgccagcgca ggacgtgcca gcggcacata caatttaacc ttaaaggccg gagaagccag
    22621 caccagcccg cgaattgcag gcgcataatc atgaacccat gtggcaacca gcactgcgcc
    22681 gacgctctgc gcgatcacta ccacctcttc cagcccgacc tggctgtcgc tggcagcaaa
    22741 acggacaaac tcatcgacat cccgcactga acgcgcaaga gatgggctat aaccacgggg
    22801 ccccgaagtt tgtccatgcc ctcgggcatc ccatgcataa aaagcagtat ctggcatcgc
    22861 cagttcatca acgatatgtt gtagacgacc agaatgttca tgaccgcgat gaaataagac
    22921 gatgaccttt ttcgcccctg gctgtaaagc gggccagtgc cgataaaaca acgctgtatt
    22981 atcactggtg gtaaaaaaat gttccccagg gatgcgtgaa ttttccattt tctctatttt
    23041 ccttcagtaa cagcagtagc gaataaaata aaaaaatacc ggagcggtga aaattaatga
    23101 gtcaatcctg tcaaggagtc cgccatgtcc tggtaatagt tttccgctat ctttaacacc
    23161 cacatctcgt ttgacggctg acatcactac atcgccgcaa aaaccactaa taccaattaa
    23221 caaacccgct aataatgcct gtaatgtatt tagcggtgtc agcaacggtc caataatcag
    23281 tgacgcaatc atggtggtga ttacgccccc caccagacct tccagcgttt ttcccgggct
    23341 gactttgggg accacttttc ttctgccgca ggatttcccc cataaatact gtgcaatatc
    23401 gtttgactct gttaacgcca aaagaaataa caccagtaac gccccgccct gggtatttgt
    23461 ggttgtcaac attaataacc aagcggcatg actcaaggcg aaaacggtca ttatccagcc
    23521 ccaaaaaatg gcgctcacgg tatgcagaaa accggaggta tcacctacta aaactcgcca
    23581 ggtggttaat atcagaaacc cagccaaagg tataaataac agaaacagct caaagcaact
    23641 aaaaccgata agcaaatagt taagaggaat aacccaatat aaccaacgcg gaaaatgtac
    23701 ggatataagc gtacaatatt ctttcaatgc cagaaaactt atcagcgcaa aaaacgtcaa
    23761 cgtcatccag cgtggacctg aaatcaccag tgaaaaacaa atgacgatac cccaccaggt
    23821 acgaatacgt agtgtaactt catccccttt tctctccggt aaccgccata cccgaaaacg
    23881 gtttatcagc gttgctaaaa ttaacaacgc gaaaagtgtt gccagagatt tttccagcat
    23941 tatctttcag cactcatcag gagcacactc cgacaacgat taatcgcagt ccagagaagc
    24001 agaattgaag caatgctcca taaaagatta ttccattgca tccactgtgg ataaatggca
    24061 acggccagcc cccacaaccc aaatattaac gcgcgatcgc ttttgccaaa aggaccgaca
    24121 taactacgaa taccgttaat cgtctgcgcg agtaaaccgc aaaactcagt caatatggtg
    24181 caaaataaca tgagtattac gagtgatgcg ttactttccg gtaaaaataa aaacggtaaa
    24241 tagagcgcaa tatcggaaat aacatcgcca gtttcattta aaatcgcccc cagacgtgtt
    24301 tgctgattgc actcacgcgc caacatgcca tccagcgcat tgagtgccat acggataaaa
    24361 agcacgatgg gcaatagcaa aaagaggatg ggttgtgcga ccaacatcaa cagcaatccg
    24421 gtaataagag aaagcgccag tgcagcaaga gtgatgtgat tcgctgtaac atggtgctta
    24481 taaagccaaa acatcgtcgg ccttaacagc gactgaaaga gcggttttat ctggtagagc
    24541 gtcattatca atccttgata attcggtttg cgctatggtg catcaattcc cctgacggag
    24601 atatcgcaac acgacgcttc attaaaaatt cagaatatat caaggtattg gggactcact
    24661 gtcaggaatg attatcccca ataaatcaag tggttaaaaa ttattacgct gttattcaat
    24721 acaactcatc aagttttctc atcgcttgtt cccgtattgc gcgcgtctta ccgttaagcg
    24781 ccagctggcg aactaaatcc agttcagggt taacaagctt taacgccagc aaaggatcac
    24841 gtttcagcct cgcctttaat acatcaactg gaaaacgggg attattcatc gccacaatcc
    24901 accactcggg atcaatttct gccgccagta ctgcaggcgg tgtgtggcgg ttgttaagtg
    24961 cgccgaactt gaaaaatgtg agattgcttt tcgccagtgt actccaccat atacgatccg
    25021 gttgttttgc cagtacatcc agttgtgcta acgcatcggg gtctagtgca taataaacac
    25081 gtaacgcttc gttaacctgc gcacgggaac tgttttcctg cctcagaaaa aacttcgccc
    25141 aaatatcatt gcgtggcgtg ttttgctgta attccttgag aacaacagcg ggtaaattat
    25201 cccaactaat taatgcactc tctaaaaagt cccagtcgtc cgaacgtatc agagaggtgg
    25261 catttaatag catctggtca tcaacttcac cgtagctgat aagctcagac aaaatcatat
    25321 cagcgctggt ttcatatgca tctgaatctg ctttgggatt atttttccga tgcgataaag
    25381 cacgttgcca aattttttgt ttcatcgacg tgctcagata ctgatttttc gccagattac
    25441 tccacccctc aggatcgttg aaacgtgtca ataaccgttg taaagcagcg tcagagtgaa
    25501 ttatctgcat atcccgacag caaaagtagg caggggattt tttctccatc cttttaacaa
    25561 aattaaactg taagttatct ggcaacgcta aaaataacgc acctattaga tcatcgtcat
    25621 tctcgaattt ttggtataaa tatactgcta ttttgtcact ctcttgtggc gtcatccgca
    25681 acaattgacc atggtgtttc atctctgcca gctttaacgc taagcttgtg acaacatcgt
    25741 ttgatgaatc gttcgccaac tgcattgcct gattgtaggt gagttgtcct ccagcaccga
    25801 cctgacgaat agaggaatca tcactgcgga tatagccatc aataatggat tcgggcaata
    25861 accacgcatc tttataaagc tcctgaatga tcatatccca atctcttttc gccagagtca
    25921 tgacgatatc cagagggaga ttttcacgta gcgcaagatt caacggaccg acaccatcag
    25981 gtagatgact cattcgcgtc attatttcga cagagagcgg ttcacggtcg agcaacccca
    26041 gaacaacagg ctggtaaccc aattgctgat cggtcaactc cagaatctca ttgtcaggca
    26101 tatatctcgc catactttca atcgtttgtc catcattgct attaataagt tgtttgcgta
    26161 ggttagctga aattccttta agccttggga gagttcgcat tatggcaaga tcaaatgtcg
    26221 caatatctcg tccttcaaat gcagcaacgt tgccttcatc cagcatttgc aattcctgca
    26281 ctaactcagg agaaggctta aggcgaaaca gcgcttcttt tttcatctga ttacagacat
    26341 tccctgccat gcgaatattg cttcgcttaa aaatttcttt cagagcatct tcatcattta
    26401 ttagacccca ataatatgca tcaggattta ttatcatcag cgcttttttg acattttcag
    26461 tgtcattttt gtcactcgca cggaccatga attcactatt tgtcgaagac gcaacaacag
    26521 gtgcaattat gtcattgata aatatcccac gcgcattaat actcccctta atcgtagacg
    26581 gattatcgta atatgagtcg ataaatacca gtccacttgc atttatcgaa ccggtaatta
    26641 acagtggtga atccgtaact atattcgttg ccgtcacgtt accgagaata acccatggat
    26701 tagaaagaga gaaagtggaa atattacctt tgactgtcaa attgccaaga acaatcaatt
    26761 tttgattact ctctatatta ccttctacca ctaaatcgcc acgatgcact ttccagtccg
    26821 atgcagataa accgaagggg acaagattca cgctactgtt gatagcttgt tcaacttgtt
    26881 gtttatcttc agcggtagcc gcagaaaaaa aaggttcaat atcaactgca tttgcaacgc
    26941 atcgaagttc cgcctgtact ggaagactca gcataaacag gggaagtaaa cctatccagc
    27001 tgcaaagcct gggcttaaac atatgcactc catgccatta aaaaagaaac aaaagggaaa
    27061 tgcatctttc ccttttgtat attcgttatt ctcgcgaaat accatccaac gtagacaaca
    27121 ctttctcaga aagatgcaac ttttcagcag ccatattctc ccgtaaatgc gcaacagaag
    27181 acgtccctgg gatcagcaaa atattcggtg aacgttgtaa cagccacgcc agcgccacct
    27241 gcattggtgt tgcacccaag ctcgcagcaa catcggaaag cgtggaagat tgcagcggcg
    27301 taaagccccc gagcggaaag aacggcacgt aggcaatgcc gtcacgagcc aaagcttcaa
    27361 tcattgcgtc atcagcacga tgagcaatgt tgtattcgtt ctgcacacag acgatttcgg
    27421 caatcttgcg cgcctctgca acctgcgtcg gtgtgacgtt gcttaggcca atatgtttta
    27481 ccaggccttg ttgttgcatc tctgccagca cggtcaggct ggcttcaatc gatccttccg
    27541 ccgggccatg accatccccc atcatcacgc gcaggttaac cacatccagt acgtccaggc
    27601 cgagattacg tagattatcg tgcaccgctt tttgcagctc tgccggagaa aatgcgggca
    27661 accaggatgc gtcctctcca cgccgcgcac caattttagt gacaattgtc agatcgtcag
    27721 agtaaggata aagcgcttcg cggataatct gattggtgac gtgcggacca taaaagtcgc
    27781 tggtatcaat gtgattgacg cccaatgcca gcgcctcacg cagcacggtt atagcgacgt
    27841 gacgatctct tggggggcca aaaaccccag gacctgccag ttgcatcgcg ccataaccaa
    27901 gacggttaac ggattttgta ccgagagtaa atgtattgct gctcatcgca ccctgccctg
    27961 taataaaacg gattaagaaa aatcagtata gaaacgaaaa gagataaata atgcgaaggc
    28021 gtaaacaaga tttctggatg agtagcacta ttgtcctgaa agacaccaaa acattagaca
    28081 gcaaaagata agtcaagcgt cgcatccggc aataaaaaac cgcaacctgt atcagcgttg
    28141 cggtttgtgt atcacatgct aaaaattaga acgttttctt aaagttcagc atcacgcctt
    28201 tgtcgctgat gccgtcctct ttccagtgat acgcatccag attcagcgaa ctttctttgc
    28261 tactgtactt cacgccgacg ctcgccaggc tgttgatacc gccgcccttc tgaccgtcat
    28321 cgacgttaaa gtgctggctg cctgcccctg caaggcttgc cgtgcgctgg ctcttgctgt
    28381 agctcaggtt cggcccgcct tccagcgtcg cattcgcgct ccagccgcct ttccctgcgt
    28441 agtccagttt cagcccgacg atgctgtcca ccgccgtttc gctgccgctg ttcatgctca
    28501 ggttaaagtc tccggcattg cgctcctgat agccgccttc cagcgagtga cgcagtttca
    28561 ctccggcgta cggggtgatt ttcagccctt cgcgcggctc aaaggttttc gccccttcgc
    28621 tacgcaactc caggtactgc tgtttcacat ccgtatccgc cgttttgctg acgtcgccgt
    28681 aagcgaccga gcggctgctg tcgagattat gcacgtcata acgcagcgcg ttgttccaac
    28741 gcataccatt gtcgaaggac atctggtgct tcaggccaaa gaactggctg tagccgccgg
    28801 ttacgccatt gtcgcccgct ttctgcgcac cgtcgccatc cagacgcgcg ataccgtatt
    28861 ccaggctcat gctctggctt tcgctcaggt caacggtttt acgcagtgcc agcatgtcat
    28921 actcggtgtt atttccgagt tccgcacgcg ggtcaccttt cgccaccacg ttaaaggcca
    28981 ggccattgcc caccttcggc gcggcatccg ccagcatgct gaagcggttg cttaacacac
    29041 gcgcttcacg gaataccgtg gtcgcctggc taccgctcac ctgcttcagg gcgctattca
    29101 gttcagcagt ggtgcccacg ttcaggctgg tatacagctc gttattggtg taacccgcat
    29161 ccagtgcctt cgccacatcg ttcaccgaag catcggtcgc cacatcggtg taggcgtttt
    29221 tgctcatgat gacgtcaacg ttgccgctgg catcggtgct gcctttggcg gtccatacca
    29281 cggacgttga ggtgatggca tcggcgtcgg tcaggttgat gccttccacc acgttgtcga
    29341 aactgaccgt ggtgtctgcc gtaccggcgg cgaaaccggt gttgatcccg acaccattca
    29401 tgctggcgtt gttgaccttc agtttaccgg cgctgccgtc aacgttggta ccgacgatat
    29461 agccgttgag gttgttactg ctgccatcgc tactaccgct atcagtcgtt tcactgaaag
    29521 gtgaagtggt gtaagtgttt ggcatgtccg gcaacgtgta gtcggtgtaa tgcacttcgg
    29581 tattgttacc gctgttgccg ttcacccctt ccacattcac gctgtcgccc tgcttaatca
    29641 accccgaacc agtcacgccg tcggcaatca ccaccgtacc gttgttaacg atatgacctt
    29701 ctgtgcccag tacgctgaac gcaaacgagt cgttagcaaa aatattgatc gtgccgttgt
    29761 tctcaattac cgcatcggcg gtggcattgg catccagttg cataccagtc aatccggtat
    29821 cggtggtgcc ttcggtaccg agattgataa cgccgttgtt aaccagctgt gcgtttttat
    29881 cgccttccat cgccacggcg ttgttgccgc tgatgttcat tacaccatcg ttgagagcaa
    29941 catagttgtt gtgggtgaat ttaaccgcgc tggcgttgtc tttatcgaag ttaattaccc
    30001 cgccatcctg gttccacaag aaggcatcgc tttctgtcgc accggcagta cttaccgcca
    30061 cggtgacttt cgcatccacc acgcccttgt taataaactc gctggcgcca tcgtcgtagt
    30121 gaatagcgcc cgcacctaat gcggcatcca gcgtcatttc accggtgtta tataacgtgg
    30181 cgttagcatt gctgccctgg aagcgaatgg cgtttttgct gttatcaagg atgagtttgc
    30241 cgctgttgtt gaacgttgac ttatcgtcaa tatccagcgt cccggcattg ttgttttcat
    30301 cctgataacc cattttccag gtacccgcgt tattcactac cgcagagtta tagagaatcg
    30361 cgcctttacg cgcctcgccg cctgcaaggt tattgaatac cgcatcgccc tgagcgttaa
    30421 cgcgcgccga cgcaataccg ctgttattca ccgtaccatc gtgaagttgt aaagttgaca
    30481 gggaaccggt gttattaatt accgcgttgc tgtttttctc aatttttacg acgtcattaa
    30541 gcgttccgtt gttattgaag gttcctcctt ccagagtcac cgtgttcaat acaccttcat
    30601 tggttaacgc accgccttca ttcacgacga tggctgagct aatggttccg ctcgactcgt
    30661 taaccagcgc accattttcg agtatcctga tcgttccatt tttaacagca cccgcactgg
    30721 tgacatcccc gttgataatc gcacctgacg ataataatac ggtctcaccc gcagaactta
    30781 atacagcgcc atcatcatat aaatcagcaa tataattatc cgttggatta tattcatcgg
    30841 ccccctagca aacgccaaag gtacaaattg tgccatagtt gataataagc ccagtgccat
    30901 cgttatagaa tgcctgacca ttttccgcgt taatgttgat tacgcctgtc tggtcattaa
    30961 tggcggtgcc ttcgtgataa accgccatgc cgacgagttt attcacaccg agagaatcgt
    31021 tgtaattttc atttttctcc aggttgatgg ttccctggtt aatgaccgta ttagtattcc
    31081 catacgccgc catccccgca ccggcgttgt agacagtaat cacaccattc tcgtggttgg
    31141 ttgccgtggc tttagttaat gccccattat aggcatcggt acctgaagcc attcccacac
    31201 cgaaatcttt cgccgtgcta tttgcaacat tattacgcag cgatgtttca tcattttcat
    31261 caacccacag cgtatccagg gtgatttgcc cttcgttatc cgcggttgat ttatcgatgg
    31321 ctagcatccc tacactgcca cggccataca tatcaataat acccgtttcg ccgttcagaa
    31381 tggatgacga gtagctggca agcatacctg tcagccctaa tgccttgtta ttgaaagagg
    31441 taccatcaga caagttccac tctgccggat aattcagcac actgtgctga ttcatcattt
    31501 tggtggtcat ggtaccattg ttaacgccaa tcccaccgta ttcaacatca aagacttttg
    31561 gtgccgggtc ggtaccaaaa tcgtacatcg ccaggccaat ggatccgtta ttgattaccg
    31621 ttgaatcttt attccaggca gccgcaaaac cgatattttt cagttccact tcaccgttgt
    31681 ttgtcgcctt cgcaccagaa tcaatactaa tggtggataa cgctacacga taataataag
    31741 tgaaagaatc cacatcactc atatcaaacg ttgcgtcagc gccaatctcc agctccgcgc
    31801 catggctcaa ctgaatgaga ttttcgaacc ccagcgggaa ctcgccgctg atggttgact
    31861 caccattcag gtagaccggg gaatgcccat caacgttaat aagcggtgtg tctccgatac
    31921 cacgtcgaga agctgatggt tttgagccat catccatgac atttttttca ccgacaaaat
    31981 ggacaccgcc attaagcgta aaggtattgt catcgccagc aataacaata cctgcaatgt
    32041 gttcctgagc accgtcgtaa ctcgcatagg aacgcgacgt ggttttacta tcagcaacaa
    32101 cggttaactg accatccagc gtgacgtcgt tgttgtcacc gctgatattt accccggttg
    32161 agggattaaa gaagtaaggc tgcgcattat cggctttctg atctttatca accagaatac
    32221 tgcccgctat ttcgatagta ttaccatcac ccgaaacatt aacacccgtc gctttttgcc
    32281 ctgtcacatt cagctcataa gtcgccagcg tcatgttgtt attattgcca gagacatcaa
    32341 gaccggtgga gaaatcccca acgtctaacc cacccgcgag tgaaatatta gctttatccc
    32401 cagcaacgct gacaccggtt ccggcattgg tcgcggaaat atcaccaacg ttaacaaatg
    32461 tcgcattatc accgtcgatc tgaatgccaa tggcattttc acccgctgcg gcctgaccaa
    32521 cattgaggtc gcccatgttg atcacgctga cgccatcacc atcaagcagc atgccaatag
    32581 agttatcttc gatgaccgta atattacctt tgttgctaac gaacgtatca ttgccgtcaa
    32641 cgtcaatacc gtgcgcacca ccgcttacgt ataagtcgcc ttcctgtttg atggtggcac
    32701 cgtcaccgac aattttcgag ccagtggaat tctctccgtc aacgctcatg gtaccttgat
    32761 tatcgacccg cgcattatcg ccattgataa ggctacccgt accaccattg gtgatggtcg
    32821 agtcgcccgc agtgtttagc ttggtattat cgccattgat gattaccgcg gtaccgccat
    32881 caacttccgt ctcgccatta ttgttaatgg tgatattgtc gccagtgagt ataccgacaa
    32941 ccgagccttc tccgatggcc gtcgttttgc cgtcgttgtt gagggagatg ttgtcaccat
    33001 caaccgtcat gacagtaccg ccattcagcg ccgtgttgcc gccctgatcg gtgatgatcg
    33061 cgttaccaaa ctcaatcgca ttcccggttg tgccgtcaac aacgacgccg gcctctttag
    33121 tgatgtggat gagtccatca tcgtcgtaac tccaggtgat gccacctgag gtatttacgc
    33181 catcaaagac aacggtgttg ctgtcgtcat ggactgtcca ggacttaact atcgtctcct
    33241 tgccctcagg cgccgttaag gtgtagctcc catcggcatt ttggctgtag gtaaaggtag
    33301 tttcgcgaat tttgacagtt tttgcatcct gatcccaggt tacgccattt ttatagacga
    33361 caggattgtg tttaaccggg gtatcgccac catcgtctgg cggaactggc gtatcaccgc
    33421 cgtcatctgg cggaaccggc gtatcaccgc cgtcatcagg cggaactggc gtatcaccgc
    33481 cgtcgtctgg cggaacaggc gtatcaccgc catcatccgg aactggggag ttattatggt
    33541 ggtgattgtc atcgtcactt aaggtatata tagcaattcc cgtagctact gcagcaagac
    33601 ctgcagctgc ccatggtaaa actgcactat ctttattttc tctgcactcc gcggataaat
    33661 tttctagctg ctctttgctc agggaatgaa tatttgccgg acatgttatc ttactggact
    33721 tacgtgtttt ttcggttgag tcgggtattg aagtatccgc agcccatgtc tggctactta
    33781 acgccatggc aacacaaaca gacaataatt ttttcctttg catataaaat tccttagatc
    33841 tagaccgtgc gtaacaagcc atgcagaatt tgaagataaa tcgtagtaaa tattcatagt
    33901 gaaataagca gtaaagaaat gcttatataa ttcacaaaat caatattaaa atcaggcaag
    33961 taaaagcata caaaaaaaac agttgatgga attatattcc acaaaaaact cacttcaatc
    34021 taaaaattaa cgacaaaaaa caaaa
//
LOCUS       DAZFBR010000044        33958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFBR010000044 DAZFBR010000000
VERSION     DAZFBR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.044"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(75..395)
                     /gene="chbB"
                     /locus_tag="ACHJZ2_002306"
     CDS             complement(75..395)
                     /gene="chbB"
                     /locus_tag="ACHJZ2_002306"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO4568400.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(695..1033)
                     /gene="osmE"
                     /locus_tag="ACHJZ2_002307"
     CDS             complement(695..1033)
                     /gene="osmE"
                     /locus_tag="ACHJZ2_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO4568401.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1235..2062
                     /gene="nadE"
                     /locus_tag="ACHJZ2_002308"
     CDS             1235..2062
                     /gene="nadE"
                     /locus_tag="ACHJZ2_002308"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO4568402.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2292..3179
                     /gene="cho"
                     /locus_tag="ACHJZ2_002309"
     CDS             2292..3179
                     /gene="cho"
                     /locus_tag="ACHJZ2_002309"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO4568403.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3139..3714)
                     /gene="ves"
                     /locus_tag="ACHJZ2_002310"
     CDS             complement(3139..3714)
                     /gene="ves"
                     /locus_tag="ACHJZ2_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO4568404.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3917..4402)
                     /gene="spy"
                     /locus_tag="ACHJZ2_002311"
     CDS             complement(3917..4402)
                     /gene="spy"
                     /locus_tag="ACHJZ2_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO4568405.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4732..5700)
                     /gene="astE"
                     /locus_tag="ACHJZ2_002312"
     CDS             complement(4732..5700)
                     /gene="astE"
                     /locus_tag="ACHJZ2_002312"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO4568406.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5693..7036)
                     /gene="astB"
                     /locus_tag="ACHJZ2_002313"
     CDS             complement(5693..7036)
                     /gene="astB"
                     /locus_tag="ACHJZ2_002313"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO4568407.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(7033..8511)
                     /gene="astD"
                     /locus_tag="ACHJZ2_002314"
     CDS             complement(7033..8511)
                     /gene="astD"
                     /locus_tag="ACHJZ2_002314"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO4568408.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8508..9542)
                     /gene="astA"
                     /locus_tag="ACHJZ2_002315"
     CDS             complement(8508..9542)
                     /gene="astA"
                     /locus_tag="ACHJZ2_002315"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO4568409.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9539..10759)
                     /gene="astC"
                     /locus_tag="ACHJZ2_002316"
     CDS             complement(9539..10759)
                     /gene="astC"
                     /locus_tag="ACHJZ2_002316"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO4568410.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            11205..12011
                     /gene="xthA"
                     /locus_tag="ACHJZ2_002317"
     CDS             11205..12011
                     /gene="xthA"
                     /locus_tag="ACHJZ2_002317"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO4568411.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12178..12888
                     /gene="ydjX"
                     /locus_tag="ACHJZ2_002318"
     CDS             12178..12888
                     /gene="ydjX"
                     /locus_tag="ACHJZ2_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO4568412.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            12893..13570
                     /gene="ydjY"
                     /locus_tag="ACHJZ2_002319"
     CDS             12893..13570
                     /gene="ydjY"
                     /locus_tag="ACHJZ2_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO4568413.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            13585..14292
                     /gene="ydjZ"
                     /locus_tag="ACHJZ2_002320"
     CDS             13585..14292
                     /gene="ydjZ"
                     /locus_tag="ACHJZ2_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFO4568414.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14292..14840
                     /gene="ynjA"
                     /locus_tag="ACHJZ2_002321"
     CDS             14292..14840
                     /gene="ynjA"
                     /locus_tag="ACHJZ2_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO4568415.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14850..16016
                     /gene="ynjB"
                     /locus_tag="ACHJZ2_002322"
     CDS             14850..16016
                     /gene="ynjB"
                     /locus_tag="ACHJZ2_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4568416.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15989..17524
                     /gene="ynjC"
                     /locus_tag="ACHJZ2_002323"
     CDS             15989..17524
                     /gene="ynjC"
                     /locus_tag="ACHJZ2_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO4568417.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17524..18177
                     /gene="ynjD"
                     /locus_tag="ACHJZ2_002324"
     CDS             17524..18177
                     /gene="ynjD"
                     /locus_tag="ACHJZ2_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4568418.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18244..19551
                     /gene="ynjE"
                     /locus_tag="ACHJZ2_002325"
     CDS             18244..19551
                     /gene="ynjE"
                     /locus_tag="ACHJZ2_002325"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO4568419.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19560..20180)
                     /gene="ynjF"
                     /locus_tag="ACHJZ2_002326"
     CDS             complement(19560..20180)
                     /gene="ynjF"
                     /locus_tag="ACHJZ2_002326"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4568420.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            20267..20674
                     /gene="nudG"
                     /locus_tag="ACHJZ2_002327"
     CDS             20267..20674
                     /gene="nudG"
                     /locus_tag="ACHJZ2_002327"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO4568421.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20640..20912)
                     /gene="ynjH"
                     /locus_tag="ACHJZ2_002328"
     CDS             complement(20640..20912)
                     /gene="ynjH"
                     /locus_tag="ACHJZ2_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO4568422.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            21148..22491
                     /gene="gdhA"
                     /locus_tag="ACHJZ2_002329"
     CDS             21148..22491
                     /gene="gdhA"
                     /locus_tag="ACHJZ2_002329"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO4568423.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22608..23648)
                     /gene="ynjI"
                     /locus_tag="ACHJZ2_002330"
     CDS             complement(22608..23648)
                     /gene="ynjI"
                     /locus_tag="ACHJZ2_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4568424.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKTMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEEQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(23776..25737)
                     /gene="topB"
                     /locus_tag="ACHJZ2_002331"
     CDS             complement(23776..25737)
                     /gene="topB"
                     /locus_tag="ACHJZ2_002331"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO4568425.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(25742..26785)
                     /gene="selD"
                     /locus_tag="ACHJZ2_002332"
     CDS             complement(25742..26785)
                     /gene="selD"
                     /locus_tag="ACHJZ2_002332"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO4568426.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(26902..27453)
                     /gene="ydjA"
                     /locus_tag="ACHJZ2_002333"
     CDS             complement(26902..27453)
                     /gene="ydjA"
                     /locus_tag="ACHJZ2_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO4568427.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            27614..29470
                     /gene="sppA"
                     /locus_tag="ACHJZ2_002334"
     CDS             27614..29470
                     /gene="sppA"
                     /locus_tag="ACHJZ2_002334"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO4568428.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            29586..30296
                     /locus_tag="ACHJZ2_002335"
     CDS             29586..30296
                     /locus_tag="ACHJZ2_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFO4568429.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            30428..31444
                     /gene="ansA"
                     /locus_tag="ACHJZ2_002336"
     CDS             30428..31444
                     /gene="ansA"
                     /locus_tag="ACHJZ2_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO4568430.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            31455..32096
                     /gene="pncA"
                     /locus_tag="ACHJZ2_002337"
     CDS             31455..32096
                     /gene="pncA"
                     /locus_tag="ACHJZ2_002337"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO4568431.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVNFT
                     VLDALQLGYKVNVITDGCHGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(32189..33448)
                     /gene="ydjE"
                     /locus_tag="ACHJZ2_002338"
                     /pseudo
     CDS             complement(32189..33448)
                     /gene="ydjE"
                     /locus_tag="ACHJZ2_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            33502..33958
                     /locus_tag="ACHJZ2_002339"
                     /pseudo
     CDS             33502..33958
                     /locus_tag="ACHJZ2_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    33783..33899
                     /locus_tag="ACHJZ2_002339"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         8254 a         8955 c         8564 g         8185 t
ORIGIN      
        1 ccttaaaaac cgcaatttaa atattgcggt attgatttat gaaataactc tttgacggga
       61 aaatttaaaa taaattaatt tgctgcggct tttttaatcg ctgcaaccgc agccttaagc
      121 acgcctaaac catcgacttt gccataaagc agcgagtcaa ttacttcaac cggtttgttg
      181 ggtaacaaac gctggatttc gggcaacata taagcaatct gcggccctaa taacacgaca
      241 tcggcattct gacctttttc accagccagt gtttccggaa atgcttcaat aatgaccgga
      301 acttcatatt tttctgcctg tgcgcgcatt tttgatacca gtaaagaggt agacatgccc
      361 gcagaacaaa acagataaat gtgtttcttt tccataaaac tgccctcatc gacgattatc
      421 tgtcagccag acactccgca agccttaacc tgcttccatg ctctgggtaa cttgcgaaac
      481 caacatgact ttttttgtgg ctgaaaggag tatacggtat agacctgagc aatagtattt
      541 ctttgacctt ataaattgtc tctccttgac ctgcggttat gaccctcttc acatttcggg
      601 caaataattc gcgaagataa ttaagaactc aggccaaaaa aaaaccggcg caatggccgg
      661 tttccgttgt tactcaagct ttcagacgaa gtgattactt cgcagcctgt ggatcagtgt
      721 cgtattcagc acaggtctga taaccggagt tgatgacatg tccggtatca tctaacgcga
      781 caaagtaggt ttctgcttta ccatcacgtt gacccaggat gtaggtctgg caagtaccac
      841 gagcatggat catgctcact tcagacgaag gtttacccgc aatttgtgca acctgcgccc
      901 ggctcatgcc ttttttgacg tctttcacca caggctgtac aaactggtct ttggtacgat
      961 cataagccgt acaacccgcc agcatggtta ataccgccgc tgcactcaga attcctgcca
     1021 tattcttgtt catattccgt cctattgttt atcagcgtgt tagataagcc tggaatacat
     1081 tgggcgcttt ttcaagccca tgaacgaaac ggctccgctt tcagaggatt cctgtatgac
     1141 gttttaacca ccattcagcc cgctgtcgct tgtcgtttca gtagcaacgg gttagcttta
     1201 aggaagtttt gtcttttctg tctggagggg ttcaatgaca ttgcaacaac aaataataaa
     1261 ggcgctgggc gcaaaaccgc agattaatgc tgaagaggaa attcgtcgta gtgtcgattt
     1321 tctgaaaagc tacctgcaaa cttatccgtt cattaaatca ctggtgctcg ggatcagcgg
     1381 cggtcaggac tccacgcttg ccggtaagct gtgccagatg gcgattaatg agctgcgcca
     1441 ggaaaccggc aacgaatcac tgcaatttat tgccgtacgc ctgccctatg gtgttcaggc
     1501 cgacgaacaa gattgccagg atgccattgc ctttattcaa ccggatcgcg tattaaccgt
     1561 taatatcaag ggcgcggtat tggctagcga gcaggcattg cgggaagcat gcattgaact
     1621 gagcgatttt gtccgtggca atgaaaaagc gcgtgagcgg atgaaagcac aatatagcat
     1681 tgcgggtatg accagcggtg tcgtggtggg caccgatcat gcagcagaag ccattaccgg
     1741 attcttcact aaatatggtg acggcggtac ggacattaat ccactgtatc gtctcaacaa
     1801 acgtcagggt aaacagttac tggcagcatt aggttgcccg gaacaccttt ataagaaagc
     1861 gccaacggcc gatctggaag atgatcgccc ttctctgcca gatgaagtgg cactcggcgt
     1921 gacctatgac aatatcgacg actatctgga agggaaaaac gtacctcaac aggtcgccag
     1981 aacaatagag aactggtatc tgaaaaccga acataaacgc cgtccgccaa ttaccgtttt
     2041 cgatgatttc tggaaaaagt aataatttgc acctattgga ttgtgcaaaa aagagtaatt
     2101 tgttcacgcc ggatgcggcg tgaacgcctt attcgaccta caaaactatg caaattcaat
     2161 acattgcagg agtcgaatag gcctgacagg cgtagcacgt cagacggtgt aacctttgtc
     2221 atcgacccgc ctctttttta atcgcttccc ggctgttaca ctggatagat aaccagcatt
     2281 cggagtcaac agtggtacgg cgtttaactt ctccgcggct cgaatttgaa gctgcggcaa
     2341 tttatgaata tcccgaacat ttacgttcat tccttaatga cttacccacc cgaccagggg
     2401 tgtatctgtt tcatggcgaa agtgacacca tgccgctcta tatcggcaaa agcgttaata
     2461 tccgcagtcg ggtgctttct catttacgca ccccggatga agccgccatg ctacggcaat
     2521 cccggcgaat tagctggatc tgcaccgcag gcgaaatcgg cgctctgctc cttgaagcgc
     2581 gattaatcaa agaacaacag ccgctgttta ataaacggtt gcgccgcaat cgccagctct
     2641 gtgccctgca attaaatgaa aagcgcgtcg atgtggtgta tgccaaagag gtggattttt
     2701 cacgagcccc caacctgttt ggcctgtttg ccaataggcg cgcagctttg caagcattgc
     2761 agagcatcgc tgatgaacaa aaactttgtt atggtctgtt gggactggaa ccgttaagtc
     2821 gcggtcgtgc gtgttttcgt tcagcgctaa aacgttgcgc cggagcatgc tgcggtaaag
     2881 agagccatga ggaacatgcg ctacgcttgc gccagtctct ggagcgtttg cgggtggtgt
     2941 gttggccttg gcaaggggcg gtggcgctga aagaacagca cccggaaatg actcaatatc
     3001 atattattca aaactggctg tggctggggg cggttaattc gctggaagaa gcgacaacgt
     3061 taattcggac acccgccggg tttgatcacg acggttataa aattctttgt aagccgctgc
     3121 tttccggtaa ctatgaaatt actgaacttg atccggcgaa tgaccagcga gccagttgat
     3181 ttcactgaac aacagtttac cttgcggttg taaaagccgc agggtgtgcc tgccatcgaa
     3241 ccagcacgcg ccctggtcgg tagtgagcag tttgtcgccc agttgccacg ccccgttaat
     3301 cacaaacacc accccgccgc gtgagccaaa ggtagtaaac gtacgttcgg caattctgac
     3361 tttcgcctta cagacatcca ggcgcgtcat aatattgaaa tccatcgaca tctgccctgc
     3421 cgtcagtttc gcttttacca cctggtccgc cgcaaaggca aaaggctgta acggttttaa
     3481 ggtatggtta aagtggtctg cgctttcaag gaacatctcg ccgccttcca gcaacgtcac
     3541 tatcctttcc ataccaggaa acaaagaaaa ctccccattc gccgcgatgg atgcaatgct
     3601 ggcacgccag taaaaatcac gttttgccgg tgggaaagtg caaatttcgc gcgtttcacc
     3661 ggcagcgttt cgccacaaat tcaccgacat tttacgcata tcaaagtatt ccatactcgc
     3721 tcccggcccg ttcttttcat tgttatcgtt caggtaaccg tggatggtta tcggcaactc
     3781 gggtgattta cgttagtggt gatcaggctt tttttacaac tgtttgagaa gagaaaagaa
     3841 aaccgccgat cctgtccacc gcattactgc aaggtagtgg acaagaccgg cggtcttaag
     3901 ttttttggct gaaagattat tcagcagttg caggcatttt accttttgcc gctggacgtt
     3961 ctgtcagacg cttctcaaaa ttagcattaa attgcttttt ctgttccggc gtcaggatgt
     4021 tgtaaatttt gttctgggtt tccatgtgcg ccagcatgtt agctttgcgc tgttcttcca
     4081 tttttgcgat ctgcgcttca gcttttgctt tatcgaaggt atcgctggca atgatgtcat
     4141 gcattgcgcg acgttcttcc agcggcggac gtttcatctg gtcacgctgg cctttcatga
     4201 tttcgcgaat ctgctgtttc tgcgcgtcgg tcaggttcag gtctttgaac atcatgtcct
     4261 gatgcggacc gaacttgcct ttgtggtgca tcatcggctt cgcgtcagcc ggtgctgcgg
     4321 tagtggtgtc tgcggcatgg gccaggttag ccgcgccaag agccagggta gaggcaacaa
     4381 acagtgcagt taatttacgc atattctata tccttccttt cagttattta ttacggcttt
     4441 cttaagtagc gtgccgtgtt gacgagatta actttactga ctttagcgtc aattaatcag
     4501 agcaacggta aaacaatgaa agtgtaaaaa acacttttgc gccaattatg gagaaaaaaa
     4561 gaaaatttga tggagagtga tgagagaata ttacaacacc atgattttgc agagattatg
     4621 aagaactata ccggatgact ggtgataaat aaagcaaata accaggatta atctgtatta
     4681 atttataaga aggtaactta atacccgcag aaagatttct gcgggtaagt attagcttat
     4741 tttttcgagc attaatcccg cgcgtaatcc cagcgctacc aacggattag ggaataacac
     4801 atactctaca tcatgggtta cggtaaaacg ttcctcaccg tcctgcgcca gcaacgttcc
     4861 tttcttaaac ggcataaaat tcagcgtgtc acttgccata tgcatttcga aggacggcga
     4921 gtggcgagta atttgcgaaa ccacccgata acggagcggc ggtgttctca cgataccgac
     4981 actctcacca gatagcagcg cagcgattgc gctggcagtt actgcaaact ggcgaagatc
     5041 gttttgccca aagggcaacg ctttgccaag ttccagcgta caggccagcg cgccaaaatg
     5101 tctggcgctg aaatgggtaa acgtaccacc aggctcctga tggaacacca gcgcctccag
     5161 ccccgccgca cccagccacg tcaggaattt ctcgtcccag ggaatgtcgc gttgcggtaa
     5221 tacaccgaac tgcggatgca aggagccacg aattgcggta tgtagatcaa ggtgccagcg
     5281 cacagattct ttgccctggt cataaaaatc ttccaggcac tgttccagtt cgcgcgcccg
     5341 acaggtttcc ccgctttcag caaacagctg ccaacgaccg ccaaacattc ggttcatatc
     5401 gctatggcaa taacgtttcc cttgcttcag cgcaggagga ttcccgagga tcaccagcaa
     5461 ccgccaccgt aacgggatct cgccgtgaga tatcgcgcca agcaatgcat caagcatctc
     5521 cacaggtgcc gtctcattac cgtggatccc cgcagaaatc accagagcgc cttgcggtgg
     5581 cgttaatggc gtcagttcca gcacaccatc gcccagccag cgccagcgaa cgccgttgat
     5641 ttctcgctcg gtgatgacag gttttttact cgttaaggtc agagcaagaa aattatccat
     5701 tgccccctcc ctcgcgctgg aacggataaa ccgaaccgag attcagtaat tggctcaata
     5761 catccagtgc ttcccgccct tcgcgcagca attgcgggtc ggccagatcg gcagcagtaa
     5821 gacgatcgcg gtagtaacga tccacccagt cattgagcgc attaaacaag gtatcgttca
     5881 tcatcaccgc cggattcacc gcccggcgtt cttcttgtgt caataccacc cgcaaccgca
     5941 ggcacgccgg gccaccgcca ttcgccatgc tttcacgtaa atcaaagact tttagttcgc
     6001 taatcgggtt gtcagcggca aggagttcat tgagataacc ccatactccg gcgtgttccc
     6061 gacactcctg aggcagcacc aacatcatgg aaccatcatc gcggctcaac agttggctgt
     6121 taaacagata tgtagacacc gcatccgaca cggaaacctg agttgccgga acttctatcg
     6181 ccataaaacc gttcacccgc gcacgcaggt ttgccagtaa ctgtgactgg cgagcgaacg
     6241 cctgttggtg gcaaaacagc acctggcggt tactcacggc aatcacgtca ttatgaaaaa
     6301 cgccctggtc gataacgtcc gggttttgct gggcgaaaat cacctgttgg ggattcacct
     6361 gattcagcct tgccaccgcc tcgctggctt cgcgagtctg tcgcgccgga taacgggaag
     6421 gccgggtatc attgccctct tctcgcccgt agacaaaaag ttgcataccc ggttcaccgt
     6481 aatgaccgcc gagacgattg tggtttgccg ccccctcatc accgagcaag gctacctgcg
     6541 gcaacgccga atggacacta aatttctctt cgtcgttaaa aatcgctttt aacagcgatt
     6601 cagtgacggg cgcttccagc gaacggtgaa atttattgtt caggttagca acggtgagat
     6661 gcactttgcc atccagcgta tcggcagatg gcgcgatcgt tgccgcattg gctacccaca
     6721 ttggcgaagc ggaactgacg ctggaaagcc agtgcggtgc ctggcgtaca accttttcca
     6781 gtacctgctc atcgctacca ctgaatccca actgacgcag caccggaata aacggacgct
     6841 cgtgaggcgg gatcacggcc tgggggaatc ccgcatcggc aagggttttc attttcagta
     6901 agccctgttt tgccgccagt cgcgggttag agacctgaaa acggtgacgg gtagaggctt
     6961 tattaccaaa cgacaggccc gcgtaatgat gcgtcagccc taccagcccg tcgaaattaa
     7021 cttcccaggc gttcatcgca ccacctcatc ggaaaaatcc agcccggggt taagcgttgc
     7081 gggcaaagtt aacgagtccg actccaggct cgccataggc catgcgcagt aatctgcggc
     7141 ataccaggcg ctggggcgat ggttaccgga tgcaccaatg ccgccgaatg gcgcggtact
     7201 ggcagcacca gtaagcggtt tgttccagtt aacaatcccc gcccgcgcct ccagcaacag
     7261 ttgatcgaat ttttcccgct cgggggaaac cagaccgcaa gagagtccga agcgagtgtt
     7321 attcgccatt cgaatcgctt cctcgaaaga atcataacgc cagacgcgca ataacggtcc
     7381 gaacacctct tcatctggta cgccagcaac gcctgtcatt tcaatgatcc ccggcgtcag
     7441 caacgatgtc cctgcttgta ataagcgcgg cgcaagcagg gttcgtccac ccatcgcttc
     7501 cagttgctgc caggcagtaa acacctgctg tgcggcctgt tcagaaatca gcccgccaat
     7561 aaacggctgc ggttcgtcat cccagttgcc cggcgttaat cgctggctga cggcaaccag
     7621 acgagcaaga aatgcatcgc cctgcgcccc gcttttcagc aatacacggc gggcgcaggt
     7681 gcagcgttgc ccggctgtaa caaacgccga ctgaatggtc agatggacag ccgcgtcgat
     7741 atccgccacc tcatcgataa tcagcgggtt attgccgccc atctcaaggg cgagaatttt
     7801 ctccggctga ccggagagct ggcgatgcag ctggtagccg gtattggcgc tgccggtaaa
     7861 cagcaagccg tcgagatcct ccagcgcact cagcgcctga cccgtttcac acccgccctg
     7921 caccaggttc agcacgcctg gcggcaagcc agcctgctgc cataaacgca ttaccgcttc
     7981 gccactccac ggtgtcagtt cgctgggttt aaagataatg gtgttacctg ccagcaatgc
     8041 gggaacgata tgtccgttcg gcaaatgacc agggaaatta tacggcccaa acaccgccag
     8101 cacgccgtgc gggcgatgtc gcaggctcgc cgcaccgtcc ggcatttcac tacgctgctc
     8161 gccggtacga acgtgatacg ccttaattga tatcgcgatt ttattgatca tcgccgtcaa
     8221 ttcggttgcc gcctcccagc gcggcttgcc ggtttctcgg gcaatgattg cggttaattc
     8281 gcctttattc ctttccagca atccggcaaa gcgttcgaca acggcctgac gttcagcaaa
     8341 tgagagccgc gcccagcgcg gaaacgccgc acgggctgcc cgacaagcct gcccgacctg
     8401 agcggcatcg gcatcattgc cttgccataa cacctcgccc gataccggat tacgcttcac
     8461 acgcgatgca ccctggcccg ttatccagtc accgttaatc cataaagtca tgctgttttc
     8521 tcctctgcgc acaggcgcac cagacgaacg cgatcccctg catggcattt gagggcatcc
     8581 agttgtgcgg cggttaaaat caaacgctcg gttgccggat cggtacgcac cagcaccacg
     8641 cggaaatggt gataattttc attggcgacc aggcaggccg gaaaatcgcc ctgcgcaggc
     8701 tgcccttctg ccacttccac cagccgactt ttacggatgg cgcgcacgcg gtcgatgtca
     8761 cactcaagcg tcggcccacc gtcgaagatg tcgatatagt tgcggtagcg aaaaccttct
     8821 ttctccagca ccgcgcgggc aggcgcggtt tgcggatgta cctgaccgat gacgtcctgg
     8881 gcttcctggg ataaaaagtg cgtatagatc ggatgtttcg gcatcagttc tgcaataaat
     8941 gccttttgcc cggtgccgca gagaaaatcg gcgcggctaa aatccatcga aaagaagcgt
     9001 ttaccgaggc tttgccagaa cggtgaatag ccgtgttcgt caatcacccc gcgcatttca
     9061 gcaaccactt tgtcattaaa cttgtcgcga aaagccgcca taaacataaa gcgcgatttc
     9121 gacagcaaat agccgttgcc ctctttgcgc cagtccgggt cgagaaacag cgtgcacagc
     9181 tcgctgctgc cggtgtgatc gttactgaga aacaacgtcg gcaatgcgtt atagacattc
     9241 agctcttttg aggcgtgaac caacgtgccg acgcgatagt tgtaccaggg atcgttcagc
     9301 ccaactgcca cctcaatggc gcaaatcccc gccacggtgc ctgtctcgct atcttccagt
     9361 acaaacacat aaccctgctc acttttgggc agttcacctt gccaggtttt gattgccctt
     9421 tcgatacgcg ccgaaagcgt ggcttcattg gcgggaagcg acgtcaggcc gccgcccgtt
     9481 ttgctggcaa gctgcatcag cgccgagaca tctgagcgct caacgggacg gatgaccatc
     9541 atgatgaacc tcggctaaca aagtgttcgc aagcaactgc aaagcgatcc agtccggtcg
     9601 tcacctcttc ttcgctgaca ttgagcgcag gcgcaaaacg caccacgttg ccacccgcaa
     9661 tcagtaccat cacgcctgct ttcgccgctt cctgagagat ctgtttcgct tgcccggcgt
     9721 aatcggcatt cagtacacag ccaatcagca aacctaagcc gcgaacttca ttgaacaaac
     9781 catagcggtg attaataata ttaagacgct caacaaacca gtcgtgacgt tgcttaaccc
     9841 cgttaagcat ctctggcgta ttgatgagct ccagcacttt gcctgccacc gccgaggcca
     9901 gcggattgcc gccataggtg gtgccatgag tgccaacggt catcacgctg gcgcactctt
     9961 cggttgctaa caacgcaccg accgggaaac cgccgcccag agctttggcg gtagttaaca
    10021 gatcaggcgt cacgccgtag tgcatatagg catacagttc cccggtacgc ccgacgccgg
    10081 tttgtacttc atcaaaaatc aacagcgcat tgtggcgatc acacaattca cgcagacctt
    10141 gtaaaaatgc gttgctggct ggcaccacac cgccttcacc ctggatgggt tcgacaatca
    10201 ccgcacaggt agagtcgtca atcagcgcgc tggcagagtt aatatcgtta tatgcagcat
    10261 gacggatatc tggtggcagt ggcgcaaaat cctgtgaata ggctggctgc ccacccgcac
    10321 taacagtaaa cagcgtgcga ccatgaaacg catttttaaa cgccacgata ccgctcttat
    10381 ggctgccgta gcggtcgtga gcgaatttac gcgccagttt tagcgccgct tcgttggctt
    10441 ccgcaccgga gttacaaaag aaaacgcgat cggcaaacgt ggcgtcgatc aattttttcg
    10501 ccagtcgcag taccgactcg ttggtgtaac cgttgccggt atgccagaac ttactcgcct
    10561 gttcgttcag cgcctcacgc agttccggat gcgcatggcc cagcgcgttc accgcaatgc
    10621 cacccgcgaa gtcgatatac tctttcccct gctgatccca caagcgcgaa ccttcgccac
    10681 gtaccggtat aaacggtgcc ggagcgtaaa caggtatcat ccattcatca aagttttcac
    10741 gcgtaattgg ctgagacata gcgacctcta cagtaaatag ctcgttattt atatgttaat
    10801 aataagtaat gttttcgctg taaatgtaga ttgcagggtt cgtgccagcc agtgatgaaa
    10861 gtgcataaac ggcggaggct aactggaaat caaggagtta taaccaaacc atatgcattt
    10921 aaagtgcata ttaagtgaat acgtttgcga tgtgggtgaa taaaaagaat aaaaaacgca
    10981 atgttatgca gaagtaaaat ataattctgg aattgtgatc attgacgaaa tttactggaa
    11041 attactgcgc cattctgacg cagcgcgcac caaaagcggg cattttttgc gccatcgttg
    11101 acatcattaa caaccatcga tcaaatcact taacaacagg cggtaagcaa agcgaaattc
    11161 tgctaccatc cacgcactct ttatctgaat aaatggcagc gactatgaaa tttgtctctt
    11221 ttaatatcaa tggcctgcgc gccagacccc accagcttga agccatcgtc gaaaagcacc
    11281 aaccggatgt gattggcctg caggagacaa aagttcatga cgatatgttt cccctcgaag
    11341 aggtggcgaa gctcggctac aacgtgtttt atcacgggca gaaaggccat tatggcgtgg
    11401 cgctgctgac caaagagacg ccgattgccg tgcgtcgcgg ctttcccggt gacgaagaag
    11461 aggcgcagcg gcggattatt atggcggaaa tcccctcgcc gctgggcaat gtcaccgtga
    11521 tcaacggtta cttcccgcag ggtgaaagcc gcgaccatcc gataaaattc ccggcaaagg
    11581 cgcagtttta tcagaatctg caaaactacc tggaaaccga actcaagcgt gaaaatccgg
    11641 tgctgattat gggcgatatg aatatcagtc ctggcgacct ggatattggc attggcgaag
    11701 agaaccgtaa gcgctggctg cgtaccggaa aatgttcttt ccttccggag gaacgcgaat
    11761 ggatggagag actgatgagc tggggattgg ttgatacctt ccgccatgcg aatccgcaaa
    11821 cagcagatcg tttctcatgg tttgattacc gctcaaaagg ttttgacgat aaccgtggtc
    11881 tgcgcatcga cctgctgctc gccagccaac cgctggcaga atgttgcgta gaaaccggca
    11941 tcgactatga aatccgcagc atggaaaaac cgtccgatca cgcccccgtc tgggcgacct
    12001 tccgccgcta atttagcagc tctcctggct caaactgggt caggagaatt aaccttgaga
    12061 aaaatcaaca aactgtcagt aatgatttgt tgcctgccgt cctttgttat accgtcactg
    12121 cgtttttagt tgtctgacca cttctctatt atcaagtttg atataggaaa ctccacgatg
    12181 aacgctgagc gtaaatttct ttttgcctgt cttatttttg cgctggccat ttacgctatc
    12241 cacgctttcg gtttattcga tctgctcacc gatttacccc acttacagac actcattcgc
    12301 cagagcggat tgttcggtta tagcctctat attctgttat tcatcattgc caccctcttt
    12361 ctgttaccag gaagcatatt ggtgatcgcg ggaggaatag tttttggccc gttcttaggg
    12421 acactactct cattaattgc cgccacgctg gcctcctcgt gctcattcct gctggcgcgc
    12481 tggatgggac gtgatttact gctgaaatac gttggccata gccatacctt ccaggccatt
    12541 gaaaaaggca ttgcgcgtaa cggtattgat tttcttattc tgacccgctt aatcccgttg
    12601 tttccttaca atattcaaaa ttacgcttac ggattaacca caatcgcctt ctggccttat
    12661 acccttattt cggcactcac gaccctgccc ggtattgtta tttataccgt gatggcaagc
    12721 gatctcgcca atgaaggcat tacgctgcgc tttattttac aactctgtct ggcgggcctg
    12781 gcgctgttta ttctcgtcca gctcgcaaaa ctctacgccc gacacaaaca tgtggatctg
    12841 tctgcttcgc gccgcaaccc acttactcac ccaaaaaatg aaggatagaa cgatgttgca
    12901 acattattca gtgtcatgga aaaaaggact gactgcactc tgcttactgg ctgttgctgg
    12961 gcttagcggc tgcgatcaaa aagagaatgc ggcggcaaaa gtggaatacg acggactttc
    13021 gaacagccaa ccgctgcgcg tcgatgccaa taaccatacg gtaaccatgc tggtgcaaat
    13081 taatggtcgt ttcctcaccg acgacactcg tcacggtatt gtgtttaaag atggctccaa
    13141 cggacataaa tcgctgttta tgggttatgc gaccccgaaa gcattttatg aagccctgaa
    13201 agaggcaggt ggtacaccgg gcgaaaacat gacgatggat aataaagaaa cgactcatgt
    13261 cacaggcagc aaactggata tttcggtcaa ctggcaaggg gcggcaaaaa cgtattcctt
    13321 cgatgaagtg attgttgaca gtaatggcaa gaaactggac atgcgctttg gcggtaattt
    13381 aacggcagca gaagagaaga aaacgggttg cctggtgtgt ctggatagct gcccggtcgg
    13441 catcgtcagc aatgcaacat acacttatgg tgcggttgaa aaacgaggtg aagttaaatt
    13501 caaaggcaat gcctcggttc tcccggcgaa taacacgctg gcaacggtta cctttaaaat
    13561 caccgaataa agccaggata aaggatgatg aagatgcaat cgcgtaaaat ctggtactac
    13621 cgtataaccc tcatcatcct gttgttcgct atgctgctgg catgggcgct gcttcccggc
    13681 gtccatgagt ttatcaatcg cagcgttgcg gcgtttgccg ccgtggacca acagggtata
    13741 gaacgcttta ttcagtctta cggcgcactg gcggcagttg tctcgttctt gttgatgatt
    13801 ttacaggcca ttgctgcacc gctacctgcg tttttgatca cctttgccaa tgcgtcgctg
    13861 tttggcgcgt tctggggcgg ctcgctgtcg tggaccagtt cgatggccgg cgcggcactg
    13921 tgctttttta tcgccagagt gatgggccgc gaagtggtgg aaaaattaac cggcaaaacc
    13981 gtacttgaca gtatggacgg ctttttcact cgctacggca aacacaccat cctggtctgt
    14041 cggttattgc cttttgtccc tttcgatcca atcagctatg ctgccggttt gacttcaata
    14101 cgttttcgct cgttttttat cgccaccggg cttggtcagt taccggcgac tattgtttat
    14161 tcctgggcgg gcagcatgtt aacaggcggt accttctggt ttgtcaccgg actgtttatt
    14221 ctgtttgccc tgaccgtggt gatttttatg gcgaagaaaa tatggcttga gcgccagaag
    14281 aggaatgcct gatgggttta ccgccgctta gcaaaattcc tttcatttta cgtccgcagg
    14341 cgtggctgca tcgtcgccat tacggcgagg tgctaagccc cattcgctgg tgggggcgga
    14401 tcccgtttat cttttatctg gtgtcgatgt ttgttggctg gctggagcgc aaacgctcac
    14461 cgctcgatcc ggtagtacga tcgcttgtca gcgcgcgcat tgcgcaaatg tgcctgtgtg
    14521 agttttgtgt ggatatcacc agtatgaaag tcgccgagcg caccggcagc agcgataaac
    14581 tgctggcagt ggctgactgg cggcaaagcc cgctctttag cgatgaagaa cggctggcgc
    14641 tggagtacgc cgaagccgca agcgtaacgc cgccaacggt cgatgatgcc ctgcgtaccc
    14701 gactggctgc gcattttgac gctcaggagc tcaccgaact gacggcattg atcggcctgc
    14761 aaaatctgtc agcccgtttt aattctgcca tggacattcc cgctcagggg ctgtgccgta
    14821 ttccggaaaa acgttcttaa ggagagatga tgcgccattg tgggtggttg ctgggattgt
    14881 tatcgctgtt ttctctggca acacatgcca gtgactggca agaaattaaa aatgaggcca
    14941 aagggcaaac tgtctggttt aacgcctggg gcggcgatac cgcaattaac cgctatctcg
    15001 actgggttag cggcgagatg aaaacccatt acgctataaa cctgaagatt gttcgtctgg
    15061 cggatgccgc agacgcggtg aagcgcattc agaccgaagc tgctgccgga cgtaaaacgg
    15121 gcggctcggt ggatctgctc tgggtgaacg gcgaaaactt ccgcacctta aaagaggcca
    15181 atttactgca aaccgactgg gcagagactc tgcccaactg gcgctatgtc gacacacagc
    15241 tgccggtgcg ggaagatttt tcagtgccta cagaaggggc tgaatcgccc tgggggggcg
    15301 cacaactgac atttatcgcc cgccgcgatg ttacgccaca gccaccacaa acgccgcaag
    15361 ccttactgga gtttgctaaa gccaatcccg gcacggttac ctatccgcgc ccaccggact
    15421 ttaccggcac ggcgtttctt gaacagttgc tgattatgct gacgcccgat cccgccgcat
    15481 taaatgaagc gccgaacgat gcgactttcg cccgtgtcac tgctcccttg tggcaatatc
    15541 ttgatgcgct gcatccgtat ttgtggcgcg aaggaaagga tttcccgcct tcgcccgcgc
    15601 ggatggatgc tctgctgaaa gccggaacat ttcgcctgtc gctgaccttt aaccccgcgc
    15661 atgcgcagca aaaaatcgcc agcggcgatt tgcctgcaag cagttacagt tttggctttc
    15721 gcgaggggat gattggcaac gtgcatttcg tcaccattcc tgccaacgcg aatgccagtg
    15781 ctgcggcgaa ggtagttgcc aatttcttgc tctcacccga tgcgcaattg cgtaaagcag
    15841 atcccgctgt ctggggcgat ccttctgttc tcgatccgca aaaactgcct gatgggcagc
    15901 gcgaaacatt gcaatcaaga atgccgcaag atctgccgcc ggtactggct gaaccgcacg
    15961 caggttgggt aaatgcgctg gaacaagaat ggctacaccg ttacggtacg cattaatctt
    16021 tttgctgtgg gcgatggtgg cggtgattta tgcaccgctg atcccggcag ctctcacgct
    16081 gatatcgcct gctttgtcgt tgacacactg gcaggcgtta tttgccgatc cacagttacc
    16141 gcaggcatta ctggcaacgc tggtgtcgac aaccatcgcg gcggtcgggg cgttgttgat
    16201 tgccctactg gtgattgtgg cgctgtggcc tgggccgaaa tggcagcgta tgtgcacccg
    16261 tctgccgtgg ctgctcgcca ttccccatgt ggcttttgcc accagcgccc ttctgctctt
    16321 tgctgacgga gggctgcttt atgactattt cccgtatttc actccgccaa tggacaaatt
    16381 gggtattggg ctgggcttca cgctggcggt gaaagaaagc gcatttctgc tgtggatctt
    16441 agcagcggta ttgagtgaaa aacggctttt acagcaagtc atcgtgctgg attcgctggg
    16501 ctacagccgc tggcaatgcc tgaactggct gctgttgccc tccgtcgcgc ctgcgctggc
    16561 aatggcgatg ctggcgattg ttgcctggtc gctgtcggtc gtagatgtgg cgattattct
    16621 tgggccaggt aatccaccga cgctggcagt aattagctgg cagtggttaa ctcagggcga
    16681 cgccgatcaa caaacgaaag gcgcactcgc cagcctgctg ctgatgttgt tactcgccgc
    16741 ctacgttttg ctgggctatc tgctatggtg tacctggcgg cgtactattc cccgcgtaga
    16801 tggcgttcgt aagcctgcca cgcctttatt gccaggcatt acgctggcga gttttttacc
    16861 cttgaccagt gtgctgtgtg tggttctgct ggcgatcctc gcggatcagt cgacgatcaa
    16921 tagtgaagcg ctcatcaaca gcctgacaat ggggctggtg gcgacattca tcgctttgct
    16981 cctgttactg ctgtggctgg aatgggggcc acagcgtcgc cagttgtggc tatggttgcc
    17041 gattttatta ccggcactgc cgctggtggc gggccagtac acgctggcgc tatggctgaa
    17101 catggatgga agctggacgg cggtggtctg ggggcatctg ctgtgggtaa tgccgtggat
    17161 gctgtttatc ctgcaacccg cctggcagcg cattgattca cggttaattt tgattgcaca
    17221 aacactgggc tggtcgcggg ccaaaatctt cttttacgtg aaatgtccgc ttatgctacg
    17281 ccctacgctg attgccttcg cggtgggatt ttcagtcagt attgcgcagt atatgccgac
    17341 gctgtggctg ggcgcggggc gttttccgac gctcaccact gaggcggtgg cattaagcag
    17401 cggcggcagc aacggtattc tcgccgccct ggctttatgg caactgctat taccgcttat
    17461 tatttttgcc ctgaccgcgt tagtcgcaaa atgggtaggt tatgtcagac aaggactccg
    17521 ctaatgctct gcgtgaaaaa tgtttcgcta cgtttaccag aaagttgctt gctgacaaac
    17581 gttaacttta cggtggataa aggtgacatt gtcacgttaa tggggccgtc tggctgtgga
    17641 aaatccactc tgttttcatg gatgattggt gcactggccg gacagttttc ttgtacaggt
    17701 gagctatggc tcaatgagca acggattgac atgctaccca ccgcacagcg tcagattggc
    17761 attctttttc aggatgcact gttatttgac cagttcagtg tcgggcaaaa tttactgctg
    17821 gcgctaccgg cgacacttaa ggggaatgcc cgacgtaatg ccgtgaatga tgcacttgag
    17881 cgttcaggcc ttgacggagc tttccatcag gatcctgcta ctttgtctgg cggtcagcga
    17941 gcgcgcgttg ctctgcttcg cgcccttctc gctcagccaa aagcgttgct actggatgag
    18001 ccattcagcc gtcttgatgt ggctctgcgc gataattttc gccagtgggt gttcagcgaa
    18061 gttcgcgaac tggcgatccc cgtcgttcag gtaacgcacg atctccagga tgttcctgct
    18121 gatagttctg ttctggatat ggcgcagtgg tcagaaaatt acaacaaact gcgataacgc
    18181 aaagtttttc tcaatgcgtc agttcagaat ggcgcactca aaactacaat gtcgggattt
    18241 tcgatgaaac gtgtttctca aatgaccgcg ctggcaatgg ctttagggct ggcttgcgct
    18301 tcttcgtggg ccgctgaact ggcgaagcct cttacacttg accagcttca acaacaaaat
    18361 ggcaaagcga tagatactcg ctccagcgcg ttttataacg gctggccaca aaccttaaat
    18421 ggcccttctg gtcatgaacc tgccgcctta aacctctctg ctagctggct tgacaaaatg
    18481 agcaccgaac agctcaacgc gtggatcaag caacataacc tgaaagccga tgccccggtg
    18541 gcgctgtacg gtaatggcaa agatgtcgac gccgtcaaaa cgcgactgca aaaagcaggt
    18601 tttacgcata tctccattct gagtgacgcg ctaagcgaac cttcccgtct acaaaaactg
    18661 ccgcactttg agcagctggt ttatccgcaa tggctgcacg acctgcaaca aggtaaagag
    18721 gttacggcga aacctgccgg tgactggaaa gtcattgaag cggccagggg cgctcctaag
    18781 ctttacctta tcagccatat tcccggtgct gactacatcg ataccaacga agtggaaagc
    18841 gaaccgctgt ggaacaaagt ttctgatgaa caactaaaag cgatgctggc aaaacacggc
    18901 attcgccatg acaccacggt cattctgtac gggcgtgacg tatacgctgc agcgcgtgtg
    18961 gcgcagatta tgctttatgc cggcgtgaaa gatgtgcgcc tgctggatgg cggctggcaa
    19021 acctggtccg acgcgggtct gcccgttgag cgcggtacgc caccgaaagt gaaagcggaa
    19081 ccggatttcg gcgtgaagat cccggcacaa ccgcagttga tgctcgatat ggaacaggcc
    19141 cgtggactgc tgcatcgcca ggatgcatcg ctggtgagca ttcgttcgtg gccagaattt
    19201 atcggtacga ccagcggtta cagctatatt aaaccaaaag gtgaaatagc cggagcacgt
    19261 tggggacacg ctggtagcga ctcgacgcat atggaagatt tccataatcc ggatggcacc
    19321 atgcgtagcg ccgatgatat taccgctatg tggaaagcat ggaatatcaa accagatcag
    19381 caagtttcat tctactgcgg caccggctgg cgcgcgtccg aaacctttat gtacgcacga
    19441 gcaatgggct ggaataacgt ctccgtttac gacggcggct ggtacgaatg gagcagcgat
    19501 ccaaaaaatc cggtagcaac cggtgaacgc ggcccggaca gcagcaaata acattgcaat
    19561 tactgacgct ggagcgactt cagcgtcaga tacccactcc acacccgcgt aaaggtcgtc
    19621 atccagcaca acgcaccgaa gatccacgca aaccacgcaa accatgcagg gaataaacat
    19681 cccagcacaa acagtaagat cgtttctgtg ccttcggtta atccccccag ataataaaac
    19741 gatttatgcg cataaccggg attatcaatc tgatatttcg ccgccagcgc agcaaaagcg
    19801 agaaaactgc tacccgtgcc gataaacgca aacagcagcc agccgcctgc cagggcgttt
    19861 tgctgcggtg cggcgaggat aaacccaaaa ggtaccagcg cgtaaaaaag aaaatcgaga
    19921 gaaatatcaa gaaagccgcc cgcgtcggta agccctctgc gccgcgccag cgcaccgtcc
    19981 agaccatcaa gcagcctgtt caacaaaatg acgacaagcg ccgccagata ccagcccagc
    20041 gccagaaaag gcagtgccag cacaccgatg gcaaatccca ctaatgttaa accatctggc
    20101 gtaatgcccg gtttatcaag aacccgcaca cactgatgca acaacggttt aatccgggga
    20161 tgaagatggc ggtctagcac aggcactcct taaatataaa gcctttctga ttgagcaaca
    20221 gtgcggatat tatggcattt ttcgcttatc tgcccgtgtg taatttatga aaatgattga
    20281 agttgttgcc gccatcattg aacgtgatgg caaaatttta ctcgcgcaac gccccgccca
    20341 gagcgatcag gcgggattat gggagtttgc cggtggtaaa gtcgagccgg atgaaagcca
    20401 gcagcaggcg ctggtgcgtg agttaaacga agaattgggc atcgaagcaa ctgtgggtga
    20461 gtatgttgcc agccatcagc gagaagtttc gggccggatt atccatcttc atgcctggca
    20521 cgtacccgac ttccacggga cgttacaggc acatgaacat caggcgctgg tctggtgctc
    20581 acctgaagag gcgctgcaat atccgctggc tcctgctgac attccattat tagaggcgtt
    20641 tatggcttta cgcgccgcca gaccagcgga ttagtgctaa gggttttgtc atcccgctgg
    20701 cattgcagca gtattccttc ggctttaatt accgcccctt cagaataatt tttatcctga
    20761 taaacgcagc actgagtaca gggctgcgct gactgcccgc ctgaactgac cacttctggc
    20821 ggtacgttta cctccacgtc cggaccataa tgcgggttag ccagtgcaat taatggaaat
    20881 gctaatacta cggcgaacaa tgctcgactc acagggaact ccttaacgtt attgtctctg
    20941 ctactgataa cggtagccgg gtggcaaaac tttagcgtct gagttatcgc atttggttat
    21001 gagattactc tcgttattaa tttgctttcc tgggtcattt ttttcttgct taccgtcaca
    21061 ttcttgatgg tatagtcgaa aactgcaaaa gcacatgaca taaacaacat aagcacaatc
    21121 gtattaatat ataagggttt tatatctatg gatcagacat attctctgga gtcattcctc
    21181 aaccatgtcc aaaagcgcga cccgaatcaa accgagttcg cgcaagccgt tcgtgaagta
    21241 atgaccacac tctggccttt tcttgaacaa aatccaaaat atcgccagat gtcattactg
    21301 gagcgtctgg ttgaaccgga gcgcgtgatc cagtttcgcg tggtatgggt tgatgatcgc
    21361 aatcaggtac aggtcaaccg tgcatggcgt gtgcagttca gctctgccat cggcccgtac
    21421 aaaggcggta tgcgcttcca tccgtcagtt aacctttcca ttcttaaatt cctcggcttc
    21481 gaacaaacct tcaaaaatgc cctgactact ctgccgatgg gcggtggtaa aggcggcagc
    21541 gatttcgacc cgaaagggaa aagcgaaggc gaagtgatgc gtttctgcca ggcgctgatg
    21601 actgaactgt atcgccacct gggcgcggat accgacgttc cggcaggtga tatcggggtt
    21661 ggtggtcgtg aagtcggctt tatggcgggg atgatgaaaa agctctccaa caataccgcc
    21721 tgcgtcttca ccggtaaggg cctttcattt ggcggcagtc ttattcgccc ggaagctacc
    21781 ggctacggtc tggtttattt cacagaagca atgctaaaac gccacggtat gggttttgaa
    21841 ggcatgcgcg tttccgtttc tggctccggc aacgtcgccc agtacgctat cgaaaaagcg
    21901 atggaatttg gtgctcgtgt gatcactgcg tcagactcca gcggcactgt agttgatgaa
    21961 agcggattca cgaaagagaa actggcacgt cttatcgaaa tcaaatccag ccgcgatggt
    22021 cgagtggcag attacgccaa agaatttggt ctggtctatc tcgaaggcca acagccgtgg
    22081 tctgtaccgg ttgatatcgc cctgccttgc gctacccaga acgaactgga tgttgacgcc
    22141 gcgcatcagc ttatcgctaa tggcgttaaa gccgtcgccg aaggggcaaa tatgccgacc
    22201 accatcgaag cgactgaact gttccagcag gcaggcgtac tatttgcacc gggtaaagcg
    22261 gctaatgctg gtggcgtcgc tacatcgggc ctggaaatgg cacaaaacgc tgcgcgcctg
    22321 ggctggaaag ccgagaaagt tgacgcacgt ttgcatcaca tcatgctgga tatccaccat
    22381 gcctgtgttg agcatggtgg tgaaggtgag caaaccaact acgtgcaggg cgcgaacatt
    22441 gccggttttg tgaaggttgc cgatgcgatg ctggcgcagg gtgtgattta agttgtaaat
    22501 gcctgatggc gctacgctta tcaggcctac aaatgggcac aattcattgc agttacgctc
    22561 taatgtaggc cgggcaagcg cagcgccccc ggcaaaattt caggcgttta tgagtattta
    22621 acggatgatg ctccccaggg aacatttctt atgggccaac ggcatttctt actgtagtgc
    22681 tcccaaaact gcttgtcgta acgataacaa gcttcaagtt cagcatccgt taactttctg
    22741 cgatagcagc agatatgcca gtaaagaaat cccattagac tatttttttg ataatcttct
    22801 tcgctttcga acaactcgtg cgcctttcga gaagcaagca ttatataatg ccaggccagt
    22861 tcttcttcaa ttgtcccgtt ttgaaaagct gtgcttgata tcgagatcat ccatgataat
    22921 tccgccgccc atattagctt cgccgaggat ttaccggagc tatgattagc gcaatcagag
    22981 atatagtctg agggaaaaac agcaaattta ttcaacaagg cgataacctg ctctggggct
    23041 tcctccatgt ttgctttaaa ggtattggct ccatggtcgc cagaaagaaa atgctccatt
    23101 aaggcacaat aactttcgct atcttcgata ccccattgtt cctctaaaga ctcgcgtctt
    23161 ttacttatga tatcgatcga gtcaaaagga agcacatgat attggaaggt atctttgcca
    23221 ggttcaggct ttcgcggcca gaactccagc gtttcagacc attgcttatg atagaatcga
    23281 taaggtgcga tcaattgtag cgcctgtaat ttctcgatac tgagcggctc aataccttta
    23341 gcctgataat aatgcagctg ttcttttttt gctttaaaac cggcccgaac aataagcccc
    23401 atcataatta atagataaag aaaagagcat cccgcggtaa tcaggcctct ttcattcaaa
    23461 ccgttggatg ttatcgctgc gaatacaaac attacagcga caacacatgt taaataaaac
    23521 ccccacttac aaagcagcat ggtcttattt tctttaatca tccgttcaaa attactatta
    23581 aatatttccc agccattaaa agaatacttc tcgctcccag gatggttttg taataaaact
    23641 tttttcatca cattccctgt tattacatac tttagaggcg ataattttat ccatgcaaaa
    23701 aaaatatcca acgaattttc ttgatccgct ggatatttca gaatatgact cgaatagcac
    23761 gaaagattcg ctcgcttacg ctatcgcccc gcttccggct tcatctgctg gcggactttt
    23821 tttcgcacta cgtttacgcg gtgcagcctt tttcttatca gcactgccac cactgcccgg
    23881 agccacaatg ccgcgaaact gccgcaccgg agtacgtttg gcttgatcaa taagctgata
    23941 tagcgtcccc accagcggct gcataaagtc ctgatagcga cactgctttt cgctgatttg
    24001 cgtcagcacc gattcccagt gcgcggtcat gtccggtcgc gtagccattt ctggcagcga
    24061 atggaataac gcttttccgg cgtcggtgga gtggatatag cgcccttttt tgatgaggaa
    24121 accacgcttg aacaacagtt caataatccc ggcacgcgtt gcttctgtcc ccagaccatc
    24181 ggtcgcacga aggatctttt tcagatcttt gtcctgcaca aagcgcgcga tcccggtcat
    24241 cgccgaaagc agtgttgcat cggtaaaatg gcgcggcggc tgggtttgcc gctctaccac
    24301 ttcacctttt tcacacagca actcatcgcc tttcgccacc acaggcaatg gcgtaccgtc
    24361 gttttcttca tcgcgctctt tgctgcctaa cagcgtgcgc cagcctgctt cagcaagaaa
    24421 acgcgcttta gcgacaaatt tgcctttggc aatgtccagt tcgataacac acttgcggaa
    24481 caccgcatcc gggcagaact gcatcagata ctgacgggaa atcaggttat agaccttcgc
    24541 ttcgttctcc gtcaggttga tcgcagaact ccgtgcggtc ggaatgatgg cgtggtgcgc
    24601 atcgaccttt ttgtcatccc aacagcggtt gcgtatatct ggatctacca ctggctgcgg
    24661 caacagatct ggtgcatgaa cgctgatggc attcatcacc gcgtggcgtc cggcaaaatg
    24721 ttcttctggc aaatagcgac aatcggaacg cggataagtg attagcttgt gcgtttcgta
    24781 cagcttctgg cagatatcaa gcacgttctg cgcgctcaga ccaaagcgtt ttgccgcttc
    24841 aatctgcaac gccgaaagcg aaaacggcag cggcgcggat tctgattccc gtttatcgtt
    24901 atagctggtg acaatagccg gttgaccact aatgcggtta accacatgct ccgccagtgg
    24961 acgatgtaac aagcgccctt cttcatcctg gtacggttca cacgcttcgc tcggttgcca
    25021 gatagcggta aaccgctcat cggcaggtgt cacgatatgt gctttgactt caaagaagtc
    25081 tttcgccacg aagttttcaa tctcttcatc gcggcgcacc accagcccaa gcacgggcgt
    25141 ctgcacgcgt cccacggaaa gcacgccctg ataaccggca ttgcgaccga gaatggtata
    25201 cgcacgggtc atattgatgc cgtacagcca gtcggcacgc gctcgcgcca gcgcagaaac
    25261 gcacagcggt acaaactcac tgttggaacg aagacggtcg atcgcccgct caaccgcctg
    25321 cgggttcagg tcgtttatca agcaacgctg cacctgctgg cgcttttccg gtgccagttg
    25381 tagatagtcc agcacttcat ccaccagcag ttgcccttcg cgatccgggt ccccggcgtg
    25441 aacgatttcg ctggcttcat gcaggtaccg tttgatgacg ttaagttgtt tggtcacgga
    25501 gggtcggggc tgtaattgcc acttttccgg gacaatcggc aaatccgcaa gattccagcg
    25561 cgcatagcgg ctgtcgtagg cgtctggctg cgcctgctca agcaggtgac cgatacacca
    25621 ggtcaccacc tgaccattac cgcactcgat aaagccatcg cctttccggt gcggtttggg
    25681 caggacatca gcaatggcgc gcgccagact cggtttttcg gcaataaaca accgcattga
    25741 attaacggat ctcaaccatg gcacgaccgc cgcgtgctgg caccagttcg ccaatcgccg
    25801 tcagttcaat gccaaactcg gcggctgtag ctttgacctc attttcggct tccggcataa
    25861 ccgccagcag caaaccgcca gaagtttgcg gatcgcacag caaatcgcgc acttcacgcg
    25921 gcatttcacc catcagatga ccgtagctgg caaagttacg ttcggtgccg ccaggcactg
    25981 cacccaactt aatgtactct tcaacccccg gcaatttcgg gatcgcgtca tagtcaacgc
    26041 gcgcctgcac acctgcgccc tgacacattt cgctcaaatg gcccagcagg ccaaagcccg
    26101 ttacgtcggt catcgctttt acgccttcaa tgttggcaaa ggacgcgcct gcgatgttca
    26161 tccggcacat cacttccgtc gccagtccct gatgttctgg tttcagcagt gattttttct
    26221 cagccgtggt aagaacgccg atccccagcg gtttcgtcag gaacagttta catccggctt
    26281 gtgcggtact gtttttcttc acccgctcgg tcggtacgat ccccgttacc gccagaccaa
    26341 aaatcggctc cggcgcatcg atggagtgac cgccagccag cgcaataccc gcctgacggc
    26401 atgcatagcg tccaccttcg gttacttcgc gggcaatttc cggggaaagt ttgttaatcg
    26461 gccagccgag gatcgcaatt gccataatcg gtttgccccc catcgcgaag atatcgctga
    26521 tggcgttagt cgccgcaatg cggccaaaat cgaaaggatt atcaacgatc ggcataaaga
    26581 agtcggtggt actgataacg ctggtgccat tgcccagatc gtacaccgcc gcatcgtcgc
    26641 gggtttcatt acccacaagc aaattcggat caacaaactt cgcctgctca ctgtgcagga
    26701 tggtttccaa cacttttggg gaaattttac agccgcaacc agctccgtgg ctgtattggg
    26761 tcaaacgaat cgagttctcg ctcatggaca tctcctgtca atgcaatccg ggtatggtaa
    26821 ccctcattcc gtgaagtgat aagtgagagt gtctgaattc ctgcgccttt gctcacaatc
    26881 cagacagttt cgcgacaatt atcagaaata agtgacaaac ggcgtcgggt ccgggacgtt
    26941 tatcgacgta gatgctttca gctgcggcgt gccgaggtag agaaaaccga caattttatc
    27001 ctgctcacgg caaccgaatg cttcacgcac taccggactt tcagttaatg cgccactgcg
    27061 ccagatgccg ccaaacccct gggcaacggc tgccatttgc atcgccatga ccgcgcatcc
    27121 ggcagacatt tcctgttccc atcgcgggac tttatgattt tcttcgcatt tcgccaccac
    27181 cgtgatgatg agcggtgcgc ggaacggcgc attacgggct ttgtcgatag ctttgtcatc
    27241 actaccggca gcaatcgccc cctgttccag tacggcgctg aaacgctcgc gcccttcccc
    27301 ttcaatcaca aaaaaatgcc acggttgcat ggacttatgg tccggcgcgc gcatacccgc
    27361 acgcaggatg ttttgcagtt gttcacccgt tggtgcgggt tcagccaggc gggaggcgct
    27421 acggcgattg atcaatagtt cgagtgcatc catttgatta actcctgtcg tgatatttat
    27481 tcacaaaatt aacacgagag tggattttgt tacagcacag tccgcaattc ctgctgacaa
    27541 gtgccggttg ggtcattacg ataaccacat ctattgcgcc tgtgacaggt gtgaccttaa
    27601 gttgggagaa tacatgcgaa ccctttggcg atttattgcc ggatttttta aatggacgtg
    27661 gcgtctgctg aatttcgtcc gtgaaatggt acttaacctg ttctttattt ttctcgtgct
    27721 ggttggtgtg gggatctgga tgcaggtcag tggtggtgat tcgaaagaaa cggccagtcg
    27781 tggtgcactg ctgctggaca tttctggtgt gatcgtcgat aaacccgaca gttctcagcg
    27841 gtttagtaaa ttaagccgcc aactacttgg tgccagttcc gatcgtctgc aggaaaactc
    27901 actgtttgat atcgtcaata ccattcgcca ggcgaaggat gaccgcaata tcaccggtat
    27961 cgtgatggat ctgaaaaact tcgcaggcgg cgaccaaccg tctatgcagt acatcggcaa
    28021 agccctgaaa gagtttcgtg acagcgggaa accggtttat gccgttggcg agaactacag
    28081 ccaggggcaa tattatctcg ccagtttcgc caataaaatt tggctgtcac cgcaaggcgt
    28141 ggtggatttg cacggttttg ctaccaacgg tctgtactac aaatcgttgc tggataagct
    28201 gaaagtttcc acccatgtgt tccgcgtggg tacgtataaa tctgccgttg aaccgtttat
    28261 tcgtgatgac atgtcaccgg cagcccgcga agctgacagc cgctggattg gtgagctgtg
    28321 gcaaaactat ctgaatactg ttgccgctaa ccggcagatc cctgctcagc aggtattccc
    28381 tggcgcgcaa gggttgcttg agggtttaac caaaaccggt ggcgataccg cgaaatatgc
    28441 actggaaaac aagctggtcg atgcactggc atcgagtgcg gaaatcgaaa aagcactgac
    28501 caaagagttc ggctggagta aggctgataa aaattatcgc gccatcagtt attacgatta
    28561 cgcattgaaa acgccggcag ataccggtga cagcatcggt gtcgtcttcg ctaatggcgc
    28621 aattatggat ggcgaggaaa ctcaggggaa tgttggcggt gataccactg cggcacaaat
    28681 ccgcgacgct cgccttgacc cgaaagtgaa agcgattgtc ctgcgtgtta atagcccagg
    28741 cggcagcgtt accgcgtctg aagtgattcg cgctgaactg gcagcagccc gggcagcggg
    28801 taagcctgtg gttgtatcga tgggcggcat ggcggcatct ggtggttact ggatttccac
    28861 gccagctaat tacattgtgg ctaaccccag caccctgacc ggttctattg gtatcttcgg
    28921 cgtgatcacc accgtagaaa atagtctgga ttcgattggt gttcatactg atggtgtctc
    28981 aacttcaccg ctggcggatg tttctatcac cagggcactg ccgccggaag cgcagcagat
    29041 gatgcagtta agcattgaga atggctataa acgctttatc acgctggttg ctgatgcgcg
    29101 tcattcgacg ccggagcaga ttgataaaat cgcccagggc cacgtctgga ccggtcagga
    29161 cgcaaaagct aacgggctgg tcgatagtct cggggatttc gatgatgcgg tcgccaaagc
    29221 agcagagctg gcaaaagcga aacagtggca tctggaatac tacgttgatg aaccgacctt
    29281 cttcgacaaa gtgatggaca acatgtctgg ttctgtccgg gcaatgttgc cagatgcgtt
    29341 ccaggccatg ttacctgcac cgctggcctc ggtagcctct accgttaaaa gtgaaagcga
    29401 caagctggcg gcgtttaacg acccacaaaa ccgttatgcg ttttgcctga cctgcgccaa
    29461 cgtgcgttaa atcctgcaaa aatgtggccg atatgctgtc ggccacactc ctgaatcgga
    29521 ctgtctttca ctccctctct ccactacatt cccttgctca tttagcatct gatatggagt
    29581 catcagtgaa caaggccccc tctttaattg ccgctatcgt cctcggactg gggattagcg
    29641 cctgtggtta ttttgttggc gatggtgtta aacatttaaa aaccaacaac cgttatgtca
    29701 atgtgcgcgg gctttctgaa aaagaagttc gcgcagatac ggcggaatta acaattgcga
    29761 ttaactttaa gggtaatgtt cccggcgaac tgttcccgaa actggaagaa gcgcagaaaa
    29821 aaattgttgc cgaactcaat gctcagggga ttaacgagaa agagataatt ctgggccaat
    29881 ggacaagcaa gcgaacagat tccttttatc tgaaagatga tcccacaatg ccacgctata
    29941 acgcagacgg gtcagtgacg ataaaaaccc ataatgttgc tgctgtcgaa aaagtcgtcg
    30001 cgaaattaaa cgaactgcaa gtcgctacgg atggcgctat cgctgagagc aaagtcgcct
    30061 atcgctttaa tggtattggc gcactgcgcg ctgagatgat tgctgccgcc accaaagatg
    30121 cacgcaacgc cgcactacaa tttgcaacgg actcaggtag ccaggtgggg tcaattagcg
    30181 atgcctcaca aggcgtattc cagatctttg ccagtggcag tgatgaagat gatcctacgg
    30241 cgattaataa aacggtacgc gtagtcacga ccgtaaccta tgcgttgcag gattaaccac
    30301 ttttctccgg cgggacacta atccctccgt cctgccggac ttttctcata tccacagagt
    30361 atccaggtgc gctcgctgcc cctcacgtat atacttttgc tctttcgata tcattcatat
    30421 caatatcatg caaaagaaat caatttacgt tgcctacacg ggcgggacca tcgggatgca
    30481 gcgttccgag cagggttata taccggtgtc aggtcatcta caacgccaac tggcgctgat
    30541 gccggaattc catcgcccgg agatgccaga tttcaccatt catgaatata cgccgctgat
    30601 ggattcttca gatatgacgc cggaagactg gcagcatatt gctgaagata ttaaagcgca
    30661 ctatgacgac tatgatggtt ttgtcattct gcacggcacc gacacgatgg cgtataccgc
    30721 ctctgcgctg tcgttcatgc tcgaaaatct cggtaaaccg gtcattgtga cagggtcaca
    30781 aatcccgctg gctgagttac gctctgacgg acaaattaat ctgctgaatg cgttgtacgt
    30841 tgcggcgaat tatccgatca acgaagtaac actctttttt aataaccgac tgtatcgcgg
    30901 caaccgcact accaaagccc atgccgatgg ttttgatgcg tttgcctctc caaaccttcc
    30961 tccgttactg gaagcaggta tccatattcg tcgtttgaat acgccacccg ccccgcacgg
    31021 tgaaggggaa ttgatcgtac atccaatcac cccacaacca attggcgtag tgacgattta
    31081 tccagggatt tctgctgacg tcgtgcgcaa ttttctgcgc caaccggtga aagcattgat
    31141 tctgcgctcc tatggcgtgg gtaatgcgcc acaaaacaaa gccttcctgc aggaattaca
    31201 agaagccagc gatcgcggta ttgtggtggt caacctgaca caatgtatgt ccggtaaagt
    31261 gaatatgggt ggttatgcca ccggtaacgc cctcgcccat gctggcgtta ttggcggtgc
    31321 agatatgact gtagaagcca cgctaaccaa actgcattac ctgcttagcc aggaactgga
    31381 tactgaaacc attcgcaagg ccatgagcca aaacctgcgc ggcgaactga cgccggatga
    31441 ttaaggagac tgcaatgccc cctcgcgccc tgttactggt cgatttacaa aatgatttct
    31501 gtgctggtgg cgcgctcgcc gtgccggaag gtgacagtac ggtggatgtc gctaaccgcc
    31561 tgattgactg gtgccagtcg cgcggtgaag cggttatcgc cagtcaggac tggcacccgg
    31621 cgaatcacgg cagttttgcc agccagcacg gtgtagagcc ttatacgcca ggccaactcg
    31681 acggtttgcc acaaaccttc tggccagatc actgtgtgca gaacagtgaa ggcgcacaat
    31741 tacatccgtt actgaaccaa aaagcgatcg cagcggtgtt ccataaaggc gaaaatcctt
    31801 tagttgacag ttacagtgcc ttttttgata acggccgtcg gcagaaaacc tctctcgatg
    31861 actggttacg cgatcatgaa atcgatgaat tgatcgttat gggcctggct actgactatt
    31921 gcgtgaattt taccgtgctg gacgcgttac agttaggtta taaggtaaac gtgattaccg
    31981 atggttgtca tggcgtgaat atccagcccc aggacagtgc gcacgcgttt atggagatgt
    32041 cagcagctgg ggcaacgcta tatacgctgg cagactggga agagacgcag gggtaatttt
    32101 acgctggcct acaattctgt actggcattg taggccaaat aaaacacgtc agtggcacat
    32161 ctggcaattg atgccatcaa cgaaagattt aatttacctc agaaatctct tccaacgaca
    32221 ccttccgcgt ttcgatgcca aaaatggaga gaaccagcgc acagagcaat aacatgacac
    32281 caagtaccat aaacaccgtg atcgacccat aatgtgttaa taatgccgca acgccatagg
    32341 gcgtgaagac tgcgacgatc cgtccgacgg cattaacgaa acccgaaccg cgcaggcgta
    32401 aatgcgttgg ccaaagctcc gggatataaa ccgccgacgc gaagcaaaca tacatgtata
    32461 aaaagaagat catcaccagg ccatagatta aaatcgccca ctctgtagtc tgaattgaat
    32521 agatatagcc taacactgca ataataataa gtaaggtgga gccaaataac cgacgaggaa
    32581 aatgatcaat aattaatgcc gcaataaata ttcctaccgg agcgccaatc ataataacag
    32641 cggtcattaa tattgattta tcgacatcaa tgccggagtt aacaaatatg gtcggtatcc
    32701 atacggtgat ggtataaagc gaaatgttca ttgcaattaa aacagtaatc gcgactaatg
    32761 tacgtcgtaa catttcacct ttaaacagga gccagaaagt acccttgatt actttaacct
    32821 tgctgttgct ctgatacgaa gtaagcgggg gtaaacgaat actcttctct ctttcaattt
    32881 gctgctctac ttcacgaagt tggcattctg cacctgcgat ttgccctttc cctgccagcc
    32941 atcgtggcga ctcaataaag tatttacttg agagaaacca ggctaacagt atgccaatac
    33001 cacccaacag aaacattatc cgccaactaa aaaaagcgat aaccaccaca cctatcgccg
    33061 cagacagcat gggcgaccag ttaccaacaa atgagagccg cgcggaccat tttccacgca
    33121 ccgtcgcggg gataaactcc gtaaatgagg catagccaac cataatcagc gcccccattc
    33181 ctgttcccat caggaagcga aagaagataa gccagtacat atcagggaca aaagcggccc
    33241 ctgttgcagc aataccgacg atgagaagat ttatgcgaaa cgccctgcgc cgcccaaagt
    33301 aatcaccgat gaacccaccg gtaagagagc cgatgaaata accaaacatt aatgccgagg
    33361 taaaggcggc attgaggaaa ttatttgacc atccattgct taccagcttt gctaagacga
    33421 cattaccgga gtaactcaaa aaccctgtga accgccccgg gaatcctgga gactaaactc
    33481 cctgagaaag aggtaaacag gatgactaaa aatacacgtt tttcccccga ggtccgtcaa
    33541 cgggcagtcc gtatggttct ggaaagtcag ggcgaatatg actcacaatg ggcggcaatt
    33601 tgttccattg ccccaaagat tggctgtaca ccagagactc tgcgtgtgtg ggttcgtcag
    33661 catgagcggg ataccggggg cggtgatggt ggactcacca ccgctgaacg tcagcgtctg
    33721 aaagagctgg aacgtgaaaa tcgtgaactg cgccgcagta acgatatcct tcgccaggct
    33781 tccgcttatt ttgcgaaggc ggagttcgac cgcctctgga aaaaataatg ccgctgctgg
    33841 ataagctgcg tgagcagtac ggggtcggac cggtatgcag tgaactgcat attgccccgt
    33901 caacgtatta ccactgtcag caacagcgac atcatccgga taaacgcagt gcccgtgc
//
LOCUS       DAZFBR010000045        33571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFBR010000045 DAZFBR010000000
VERSION     DAZFBR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.045"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(129..1310)
                     /locus_tag="ACHJZ2_002340"
     CDS             complement(129..1310)
                     /locus_tag="ACHJZ2_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4568432.1"
                     /translation="MALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEA
                     VAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLTD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
                     PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEL
                     A"
     gene            complement(1506..1600)
                     /locus_tag="ACHJZ2_002341"
     tRNA            complement(1506..1600)
                     /locus_tag="ACHJZ2_002341"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1564..1566),aa:Sec,seq:tca)
     gene            1893..3275
                     /locus_tag="ACHJZ2_002342"
     CDS             1893..3275
                     /locus_tag="ACHJZ2_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO4568433.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3285..5603
                     /gene="yicI"
                     /locus_tag="ACHJZ2_002343"
     CDS             3285..5603
                     /gene="yicI"
                     /locus_tag="ACHJZ2_002343"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO4568434.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(5656..7365)
                     /locus_tag="ACHJZ2_002344"
     CDS             complement(5656..7365)
                     /locus_tag="ACHJZ2_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4568435.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(7486..8877)
                     /gene="xanP"
                     /locus_tag="ACHJZ2_002345"
     CDS             complement(7486..8877)
                     /gene="xanP"
                     /locus_tag="ACHJZ2_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO4568436.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9157..10362
                     /gene="gltS"
                     /locus_tag="ACHJZ2_002346"
     CDS             9157..10362
                     /gene="gltS"
                     /locus_tag="ACHJZ2_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO4568437.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10365..11231
                     /locus_tag="ACHJZ2_002347"
     CDS             10365..11231
                     /locus_tag="ACHJZ2_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568438.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11216..13297)
                     /gene="recG"
                     /locus_tag="ACHJZ2_002348"
     CDS             complement(11216..13297)
                     /gene="recG"
                     /locus_tag="ACHJZ2_002348"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO4568439.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13303..13992)
                     /gene="trmH"
                     /locus_tag="ACHJZ2_002349"
     CDS             complement(13303..13992)
                     /gene="trmH"
                     /locus_tag="ACHJZ2_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO4568440.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(13999..16107)
                     /gene="spoT"
                     /locus_tag="ACHJZ2_002350"
     CDS             complement(13999..16107)
                     /gene="spoT"
                     /locus_tag="ACHJZ2_002350"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO4568441.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16126..16401)
                     /gene="rpoZ"
                     /locus_tag="ACHJZ2_002351"
     CDS             complement(16126..16401)
                     /gene="rpoZ"
                     /locus_tag="ACHJZ2_002351"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO4568442.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16456..17079)
                     /gene="gmk"
                     /locus_tag="ACHJZ2_002352"
     CDS             complement(16456..17079)
                     /gene="gmk"
                     /locus_tag="ACHJZ2_002352"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO4568443.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17337..19019
                     /gene="ligB"
                     /locus_tag="ACHJZ2_002353"
     CDS             17337..19019
                     /gene="ligB"
                     /locus_tag="ACHJZ2_002353"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO4568444.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(19016..19633)
                     /locus_tag="ACHJZ2_002354"
     CDS             complement(19016..19633)
                     /locus_tag="ACHJZ2_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO4568445.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(19925..20749)
                     /gene="dinD"
                     /locus_tag="ACHJZ2_002355"
     CDS             complement(19925..20749)
                     /gene="dinD"
                     /locus_tag="ACHJZ2_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO4568446.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(20970..21833)
                     /locus_tag="ACHJZ2_002356"
     CDS             complement(20970..21833)
                     /locus_tag="ACHJZ2_002356"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO4568447.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIVQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21960..22676
                     /gene="rph"
                     /locus_tag="ACHJZ2_002357"
     CDS             21960..22676
                     /gene="rph"
                     /locus_tag="ACHJZ2_002357"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO4568448.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22742..23383
                     /gene="pyrE"
                     /locus_tag="ACHJZ2_002358"
     CDS             22742..23383
                     /gene="pyrE"
                     /locus_tag="ACHJZ2_002358"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO4568449.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(23420..24016)
                     /gene="slmA"
                     /locus_tag="ACHJZ2_002359"
     CDS             complement(23420..24016)
                     /gene="slmA"
                     /locus_tag="ACHJZ2_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO4568450.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(24123..24581)
                     /gene="dut"
                     /locus_tag="ACHJZ2_002360"
     CDS             complement(24123..24581)
                     /gene="dut"
                     /locus_tag="ACHJZ2_002360"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO4568451.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24559..25779)
                     /gene="coaBC"
                     /locus_tag="ACHJZ2_002361"
     CDS             complement(24559..25779)
                     /gene="coaBC"
                     /locus_tag="ACHJZ2_002361"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO4568452.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25951..26619
                     /gene="radC"
                     /locus_tag="ACHJZ2_002362"
     CDS             25951..26619
                     /gene="radC"
                     /locus_tag="ACHJZ2_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO4568453.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26836..27072
                     /gene="rpmB"
                     /locus_tag="ACHJZ2_002363"
     CDS             26836..27072
                     /gene="rpmB"
                     /locus_tag="ACHJZ2_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO4568454.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            27093..27260
                     /gene="rpmG"
                     /locus_tag="ACHJZ2_002364"
     CDS             27093..27260
                     /gene="rpmG"
                     /locus_tag="ACHJZ2_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO4568455.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            27358..28167
                     /gene="mutM"
                     /locus_tag="ACHJZ2_002365"
     CDS             27358..28167
                     /gene="mutM"
                     /locus_tag="ACHJZ2_002365"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO4568456.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(28206..28685)
                     /gene="coaD"
                     /locus_tag="ACHJZ2_002366"
     CDS             complement(28206..28685)
                     /gene="coaD"
                     /locus_tag="ACHJZ2_002366"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO4568457.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28693..29970)
                     /gene="waaA"
                     /locus_tag="ACHJZ2_002367"
     CDS             complement(28693..29970)
                     /gene="waaA"
                     /locus_tag="ACHJZ2_002367"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO4568458.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            30383..31441
                     /gene="rfaQ"
                     /locus_tag="ACHJZ2_002368"
     CDS             30383..31441
                     /gene="rfaQ"
                     /locus_tag="ACHJZ2_002368"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO4568459.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            31438..32562
                     /locus_tag="ACHJZ2_002369"
     CDS             31438..32562
                     /locus_tag="ACHJZ2_002369"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4568460.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            32555..33352
                     /gene="rfaP"
                     /locus_tag="ACHJZ2_002370"
     CDS             32555..33352
                     /gene="rfaP"
                     /locus_tag="ACHJZ2_002370"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO4568461.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7940 a         8325 c         8928 g         8378 t
ORIGIN      
        1 acctgttttg tgttgtggtg aggatatcac ctttaatcag gtggccaaat ttactgtgcc
       61 actacaacca tccgcctgtt ggggctggat ggttattcat tatttttgtg tcaccgaact
      121 ttcattcatc aggccagctc agaaagtgaa tcaatgtagt cagcgtacca ttgcatcatt
      181 tcacggcgac catcgagata ctgggcgtgg ttataggtac ccctgatgga gtttttatcc
      241 acatgagcca actgcatttc aatccaggca gaattaaagc cttgttcgtg cagaattgtg
      301 ctcatggtat gcctgaaacc gtgccctgtg agtcttccct tataccccag caattcaatg
      361 acctggttaa tgctctcctt actaattggc ttgcgaggat cgttcctacc gataaagacc
      421 agaggatgat gttttgagat tggttcaagt tgcttaaata gcgcaataac ctgctcagag
      481 ggcggcacaa tatgaggccg tttcatcttc attacttctg ccgggatctc ccataatttt
      541 gcctcaaaat caatatcctc ccaatgcgcg aaacgcaact cttgcgttcg tacaccggtt
      601 aacataatga tctgagtagc tgtcttagtg atgatacttc cggtataacc cgccagatca
      661 gtaaggaagt ggggaagttc attggcagta agaaagggaa aatgtacttt tttcggtgta
      721 gctaaagcac tggcaagatc gggagcaggg ttatagtcgg ctcgaccagt aacaattgca
      781 tagcgaaaca cctcaccaca acgttggcgg accttgcgca ttttctccag tgctccacgt
      841 ttctccatct tacgtagtgc ttccaatagc tccatcggct tgatttcggc aataggtcgc
      901 ttgccgatgt aggggaagat atctttctca aaggtgtcga ttatttcatc gcggtagcgc
      961 agtgaccagc gatcggcttt cgtctgatgc cactcgcgag caacggcctc gaaagtgttt
     1021 ttcaaactca tttgcagcgc tattttttct tctttttttg cctcacccgg atcgccacca
     1081 gccgcaagta ttttcctggc ttcactgcgt ttctcacgtg cgtcggcaag cgttacatcg
     1141 gggtatacgc caatagacag catcttttct ttaccggcga agcggtactt catacgccag
     1201 tatttggaac cattagcgtt aatcagcaga tacatacctc cgccatcagc cagcttatag
     1261 ggcctttctt ttggcttcgc ggttttcact ttaacatcgg ttagcgccat gacatcctcc
     1321 agcttttgat gaatccatcc tcctggggga atttccgcca gtaaccaacc tgataccccc
     1381 aatgcacccc cacaaacaac ttgatatggg ttaaagtcgt ttgacctcag gatacaaaga
     1441 aaacctgata tataagggat tataaggggc tttgttgatg tcgggagagt tcaggagagg
     1501 caaactggcg gaagatcaca ggagtcgaac ctgcccggga ccgctggcgg ccccaactgg
     1561 atttgaagtc cagccgcctc accggagacg acgatcttcc gcgcctcgat tgctacatgg
     1621 aggcggggcg cattatagct acttccttga gtttctacat cccccagatc agttttttcc
     1681 gcctaataca cgcagcgtat tatcacgaaa gcattataat tcatataaaa ctcatatttt
     1741 acaatgatat atgagcggaa ttaacgctac cacgatcaca taaaacaaga atcataaatt
     1801 aataaccaga tatcggaata ttcgctctca cagggatggt ttacaaaatg cgttatcggt
     1861 ctcagcaccc ctatagcatc aaggaaaagc agatgaagag tgaagtgttg tccgttaaag
     1921 agaaaattgg ttatggcatg ggagacgccg ccagccacat tattttcgat aacgtaatgt
     1981 tatatatgat gttcttttat accgataatt ttggcatacc tgccggattt gtcggaacca
     2041 tgtttttggt cgctcgtgca ctggatgcga tttccgatcc ttgcatgggg ttgttggccg
     2101 atcgaacgcg ctctcgctgg ggtaaatttc gtccgtgggt actgtgtggc gcactgccat
     2161 ttgggatcgt ctgtgtactg gcctatagca cgccagatct cagtatgaac ggcaaaatga
     2221 tctatgcagc aattacttac accctactta ccttacttta taccgtcgtc aatatccctt
     2281 actgcgcatt gggtggtgta atcaccaatg acccgactca gcgtatctcg ctgcaatcct
     2341 ggcgttttgt gctggcgacc gcgggaggca tgctctctac tgttctgatg atgccactgg
     2401 ttaatttaat tggcggtgat aataaaccac tcggtttcca gggcggtatc gcggtccttt
     2461 ccgtggtggc attcatgatg ctggcattct gtttcttcac cactaaagaa cgcgttgaag
     2521 caccacctac aacgacgtct atgcgggaag atttacgtga tatctggcaa aacgaccagt
     2581 ggcggattgt cggtttacta accattttca atatcctggc ggtgtgcgtc cgcggtggag
     2641 cgatgatgta ttacgtcaca tggattttgg gtacgccgga agtgtttgtc gcttttctca
     2701 ccacttattg cgtgggtaac ctgattggtt ccgcactggc aaaaccgctg accgactgga
     2761 aatgtaaagt cactatcttc tggtggacga acgccctgct ggcagtgatt agcctcgcga
     2821 tgttctttgt tcctatgcag gccagcatca ctatgtttgt cttcatcttc gtgattggtg
     2881 tattgcatca actggtgaca cctatccagt gggtaatgat gtccgatacc gtcgactacg
     2941 gcgagtggtg caatggtaaa cgcctgaccg ggatcagttt tgctggcacg ctgtttgtgc
     3001 tcaaactggg gttggccttc ggcggcgctc ttatcggctg gatgctggct tatggcggat
     3061 atgatgcggc agaaaaagcg cagaacagcg ccacgattag catcattatt gcgctattca
     3121 cgattgttcc ggcgatctgt tatttgctga gcgcgattat cgctaaacgc tactactcac
     3181 tcacgacgca caatctgaaa accgttatgg aacagctggc ccagggcaaa cgccgttgcc
     3241 agcaacaatt cacctctcaa gaagtgcaga actaaggaac ggcaatgaaa attagcgacg
     3301 gaaactggtt gattcaacct ggcctcaatt tgattcaccc gcttcaggtg ttcgaggttg
     3361 aacagcaggg taatgaaatg gtggtctatg ctgccccccg tgatgtgcgt gaacgaacct
     3421 ggcagcttga tacgccttta tttacgctgc gctttttctc cccacaggaa ggtattgtcg
     3481 gtgtacggat tgagcatttt cagggggcgc tgaataacgg tcctcattat ccgctcaata
     3541 ttttgcagga cgtgaaggtc acaatcgaaa acacagaacg ttacgctgag tttaaaagtg
     3601 gcaacttaag cgcgcgtgtc agcaaaggtg agttctggtc actggatttt ctgcgcaacg
     3661 gcgaacgtat taccggtagt caggtgaaaa ataatggcta cgtgcaggac acgaataatc
     3721 aacgaaatta tatgtttgag cggctggatc ttggcgttgg cgaaacagtt tacggtctgg
     3781 gagagcgctt tactgccctg gtgcgcaatg gccagacggt agagacctgg aaccgggacg
     3841 gcggcacaag tactgaacag gcgtataaaa atatcccgtt ctatatgact aaccgtggct
     3901 atggggtact ggtcaatcat cctcaatgcg tctcttttga agtgggatcg gagaaagtct
     3961 ccaaagtgca gttcagcgtt gagagtgaat atctcgaata ctttgttatc gacggcccga
     4021 cgccgaaagc ggtacttgat cgttataccc gttttactgg tcgtccggcg ctgccgcccg
     4081 cgtggtcctt cggcctgtgg ctaaccactt catttaccac caactacgac gaagcgacgg
     4141 taaacagctt tatcgatggt atggcgggac gcaatctgcc actgcatgtt tttcactttg
     4201 actgtttctg gatgaaagcc ttccagtggt gcgattttga gtgggacccg ctgactttcc
     4261 cggacccgga agggatgatc cgccgcctga aagcgaaagg gctaaaaatc tgcgtctgga
     4321 ttaaccccta tatcgggcaa aaatcccctg tctttaaaga gttacaagag aaaggctatt
     4381 tactcaaacg cccggacggt tcgttatggc agtgggataa atggcagcca ggtctggcga
     4441 tttatgactt taccaatccg gatgcctgca aatggtacgc cgacaaactg aaaggtctgg
     4501 tcgcgatggg cgttgattgc tttaagaccg actttggcga acgtattcca atcgatgttc
     4561 agtggtttga cggttccgat ccgcagaaaa tgcataacca ttatgcgtac atctacaacg
     4621 aactggtgtg gaacgtgctc aaggacaccg ttggcgagga agaagccgtc ttgtttgctc
     4681 gctcggcctc cgttggtgcg cagaaattcc cggtacactg gggtggtgac tgttacgcta
     4741 actacgaatc aatggcggaa agcctgcgcg gtggtttgtc tattggcctt tcaggttttg
     4801 gtttctggag ccacgatatc ggcggctttg agaataccgc tctggcgcac gtttacaaac
     4861 gctggtgcgc gtttggtttg ctctccagcc atagccgttt acatggcagc aaatcttatc
     4921 gtgtgccgtg ggcctacgat gatgagtcct gtgatgtggt gcgcttcttc acgcaactga
     4981 aatgccgcat gatgccgtat ctgtatcgtg aagctgctcg tgcgaacgcg cggggtacgc
     5041 cgatgatgcg ggccatgatg atggagttcc cggacgatcc ggcttgtgat taccttgacc
     5101 gtcaatacat gttaggcgac aacgtgatgg ttgctccggt gttcagtgaa gcgggcgatg
     5161 tgcagttcta cttgccggaa ggtcgctgga cacatctgtg gcacaacgat gaactcgatg
     5221 gtagtcgctg gcataaacag cagcacagct tcctgagtct gcccgtttat gtgcgtgata
     5281 acaccctact ggcgctgggc aacaacgagc aacgtcccga ttacgcgtgg cacgaaggca
     5341 cggcattcca gctctttaat ctacaagacg ggcatgaagc catctgtgaa gtgcccgctg
     5401 ctgacggttc cgttcttttc accctgaaag cggcgcgtac tggcaacacg attactgtga
     5461 ctggtgcggg cgaggcgaag aactggacac tgtgcttgcg caatattgtg aaagtaaatg
     5521 gtctgcaagg tggttcgcag gctgaaagtg agcaggggct ggtggtgacg cctcaaggga
     5581 atgcgctgac aattacgttg taatctattg atggaatgcc gggtacgtct gagtcgtacc
     5641 cggcgagatt gatttttact gcaccggctc tgtcgatgta acttcgctac tggaaacgac
     5701 gcccgcattc atggtttgag tcacttgctg ctttgcggcg ttcacggcat ggagttgccc
     5761 gctaaccgta ggtttcagcg ggacattggc ctgaatatcg ccactggcgg taagctgaat
     5821 attgccgtcg ccagtcaacg gtaattcagg ccatccccat tgttgcaaaa tattcaccgg
     5881 cacaccgcgt ccattcaggc tgatatgggt ctgacgttgt ggcgtttgtg aaacactggc
     5941 agtggcttcc agaatgcctt tttcagtaaa tgcactcagt tcgctgatat tcaccgtgct
     6001 gctgttggcg gtcagcgcca gcgacgggcg acgaacatca acacgattga atgtcgcggc
     6061 ggcggcattc agattcgccg agccactcca gacgccccat ttatgatcgg taaccagcgt
     6121 caggttggca ccgtaaccat cgagcgtggt gagctgccac gggaagtcag ggtcgatatc
     6181 aatgatcaga ttgcggctgg cgctaaatct cttcagttgc aggctgttta accaaccggg
     6241 tgtcgtttcc atccacaact gttgccagtt tttcggcaag gtatattcca gcccggcaat
     6301 tgccgcatca tcaaggatca acgttttccc gtcacgcagc cagttccctg acgttctgac
     6361 cataccccct tcccagcggc tggtgaactg gcgcagcgct acgccctgcg gggaaaattc
     6421 cgcgtttata atcgggtcaa ataaatgcag cgaaccataa atgaactcgc tagcgttcat
     6481 cgacagtttg ccttcttgtg tctgccagtc atctttactg aaggtcatgt tgcgcaagct
     6541 gagatcgagg tcggtcaccg cccagtccgg accttgcaaa cgagcatcga tcacttccag
     6601 gcgaccaatc tgcaacgacg ggacagagcg taatggcgca aagaagtcgg ttagcgattt
     6661 ttcgctttgt agacggatat ccgccatgcg cagattttcc acttgccagc tgccgtcggc
     6721 gttacgctgc gcgtttccgg ttaatgtccc gcgggcgatg tcggcaccca ggttagtcag
     6781 cgtaacgcga tcgttatcaa tactgccttc aatcagtaca ttggtggcag gaacatcatt
     6841 gagcgaaagc gatccggcac taaactgaat ctgcgccttc gtacccagca ctttaccggc
     6901 ttctggtgac cacggaacca cgccgccatt tacccgctgc gcgctcagtt tccattcgct
     6961 attcgggcta ttaaacgcca tatcacgcag ttgcagacga tcggctttga acggtagcgg
     7021 cgcggtctgg tcggtgagat tcagcgtccc gttttccagc aggatggtat cgacatggcg
     7081 tggttcggtc agttgccgac tgcttagcgc aatgtcgaca ctttttgcca ccagggtcgc
     7141 gggctgacca tcacgaccaa acgtgacgtt ctccagcacg atatgagatg gcgcggaaaa
     7201 acggtgatcc atcgccccga aggccagatg atagtcgcta ttctcggaaa cccatgcgct
     7261 gatatgttct gctccccagc gggtttgcag aagaaaatag aggccagcga tcaccactaa
     7321 cagagcgatg agaatgtaga gaagcagctt cccaataaat ttcatggtct tccatcccgt
     7381 gaaatgcaca taagggagtt atgcacgatt tacgcgcaat cctcaaggcg ggaatggtga
     7441 aagaggtgag actgcggcgg taattatctt accgccgcca gtgaattact gtttttctgg
     7501 tgggaaaatc agattcagca cgatggcagt aataccgccc gcggcgatcc cggaggagag
     7561 caggtttttc agccattcag gggcaaactg caaaatcagc ggctgctgag acacgcccag
     7621 accaaccgcc agcgacagcg cgataatcag aatcgcccga cggttcagcg gctcacgaga
     7681 aacgatacgc acaccggagg cggcgatggt gccaaacatt acaagcgttg cgccgcccag
     7741 aaccggttct ggaatgtgtt gtacaaaacc gctcactgcc gggaacagac ccagcacgat
     7801 caacatcagc gcgacgacaa aaccgacata gcggctggca acaccagtca actggatcac
     7861 tccgttgttt tgcccgaagc aggagttcgg gaaggtgtta aacaccgccg aaacaaacga
     7921 gttcaggccg tttgccagca cgccgccttt caggcgtttc atgtacagcg gaccggacac
     7981 tggctgttcg gaaacgtcag aggtcgccgt gatatcgcca atggtttcca gcgaagtgat
     8041 cataaagacc agcatcagcg gcagcagcag actccattca atgccaagac cgtaatagag
     8101 cggcgttggc accataatca gttcttgcgt catcggttcg ttgctttctg gcaacatgcc
     8161 cataaaccac gccagcgcat atccggccgc catcgcaatc accagtgagg ccacgcgtaa
     8221 gtaagggtta cgttgacggt taagcaggat aattaaggct aagaccacgc ctgccagcag
     8281 cagatttttc ggtgcgccga aggtgttatc gctcatggct gcgtaaccgc cgccaatgga
     8341 cgttaaccca acctgaatta gcgacaggcc gataatcatc accacaacgc cagaaaccag
     8401 cggcgtaata atgcggcgcg ccagatgcag aacgcgggag atcaccatct cggtgcaact
     8461 tgccagcatc aacgtgccga acaaagccgc catcatggta ggaacatcag caccaccggt
     8521 tttcagcgcg gtaccgccca taatcagcgg ggcaacaaag ttgaagctgg tgccctgaat
     8581 agacaacagc ccggagccaa ccggacccca ggccttaatt tgaataatcg atgccacacc
     8641 ggaggcaaac agcgacatac taataatgtg ttgcgtgtct tgtgccggta aacccagcgc
     8701 ctggcagatt aatagcgctg gcgtgatcac cgcaacgaac atcgccagca gatgctgaca
     8761 ggcggcaaac agggtttgag gaagcggcgg acgatcttca agacggtaaa tcagttcgct
     8821 gttttgagtc tgcgcaaccg gttgcgcatt ttctgactcg agggtggaaa cagacatcga
     8881 aaacaatccc gtggtagcaa agcgggcatt ttagctgacc gaaagagaaa agcaaacgtt
     8941 tgccacatcc tcatctaaat atttaaatga gataaatgca cttttttata taacttttgg
     9001 tatttttctg cttaaaatcc atgccatttg ttcattaaca gctgagaatt tgacccagga
     9061 aagcataact cgatacccgt catagttcat gaagttgcca ctgcgcgatg aagtatgacg
     9121 agtatgaaag agtgatgcgg atacaaagga gtaactatgt ttcatctcga tactttagca
     9181 acgcttgttg ccgcaacgct gacgttgttg ctcgggcgta agttggtcca ttccgtctcc
     9241 tttttgaaga aatacaccat accggaacct gttgcgggtg gtttgttggt ggcgctggcg
     9301 ctactagtac tgaaaaaaag catgggctgg gaagtcaact ttgatatgtc cctgcgcgat
     9361 ccgttaatgc tggctttctt cgccaccatt ggcctgaacg ccaacattgc cagtttgcgt
     9421 gccggtgggc gtgtggttgg catcttcttg attgtggttg ttggtctgtt ggtgatgcaa
     9481 aatgccattg gcattggtat ggctagcctg ttagggcttg atccgctgat ggggctgttg
     9541 gccggttcta ttacgctttc cggcggtcac ggtacgggcg ctgcgtggag taaattgttc
     9601 attgaacgtt atggcttcac caatgcgacg gaagtggcga tggcctgtgc aacgttcggt
     9661 ctggtgctgg gcggcttgat tggcggtccg gtggcgcgct atctggtgaa acactccacc
     9721 acgccgaacg gtattccgga tgaccaggaa gtcccgacgg cgtttgaaaa gccggatgtg
     9781 ggccgcatga tcacctcgct ggtgctgatt gaaactatcg cgctgattgc tatctgcctg
     9841 acggtgggga aaattgttgc gcaacttttg gctggcactg cttttgaact gccgaccttc
     9901 gtctgtgtac tgtttgttgg cgtgattctg agcaacggtc tgtcaatgat gggcttttac
     9961 cgcgtctttg agcgtgcggt atccgtgctg ggtaacgtaa gcctgtcgtt gttcctggcg
    10021 atggcgctga tggggctgaa actgtgggag ctggcttcgc tggcgctgcc gatgctggcg
    10081 attctggtgg tacagaccat cttcatggcg ttgtatgcca tcttcgttac ctggcgcatg
    10141 atgggcaaaa actacgatgc ggcagtgctg gctgcgggtc actgtggttt tggcctcggt
    10201 gcaacgccaa cggcaatcgc caacatgcag gcgatcactg aacgctttgg cccgtcgcac
    10261 atggcgtttt tggtggtgcc gatggtcggt gcgttcttta tcgatatcgt caatgcgctg
    10321 gtgattaagc tgtatttaat gttgccgatt tttgccggtt aaaaatgaaa aagcgtcgga
    10381 aggaaccgga aacgctacgt gaacactgtc gccatatttt tggtgatgag ccgccagtgc
    10441 tctgtgtatg ggaaacggaa tttgattacg cagatgccga actcaaggcg ctggcggcta
    10501 aagagtggca gcagatcagc gaatgggatt tgagtgctta ctatgtactc aatctcgttt
    10561 ataacgaacc gatgcaaata gagttgtttc gctatctgtt tccgctctgc ctggcgcaat
    10621 ggcatgaaac cgttttagct ggcggttatg gtgatcactt cgaagaatcc ttaatgaagg
    10681 cattgtgtcg gccctatctc tggcaggaga tgatgaatgc ttcccaacgc cagcaggtac
    10741 ggcaattttt actggacacc gcgttgcagc gtatggataa cgagcgtggt ttcaataacg
    10801 ttctttgctg gctggcggtg ttcaatactc tgggtggggc cgcgccgtta attcgttctc
    10861 tctggtcgcg ctggtgggcg ctggatacgc caggaaaagc ggtgtgtgct attcagtatg
    10921 ccgcgcatct aatttatccc atagaagcca atccgttatg gtcgcaagaa tggataggct
    10981 gggggcatcc attaggccat aaagatggct ggtcctctga taatcgcgct tttcttcgcc
    11041 aaatgttaac acctgaaatg attgtggctg gcgtacaggc agcggctgaa attctgcgtg
    11101 gtgaaccgga aggcgcaatg gcggcgcgaa ttgcacagga tgcgtatgag gcaatggata
    11161 tcctcactat ccagattgaa gatttactgc gtgacttgtc ttgtgatgag tctggtcatg
    11221 cgttggagta acgttccgtc tccggcatcc agcgttctat cagggctttt gcttgttgtg
    11281 ggtaacgttc gtgaatatgt cgcgccaggc gctgaacttc cgggatcatc gcctgatcgc
    11341 gcagtaaatc cgccacttta aactcagcat tacccgtctg acgcgtacct aacagttcac
    11401 ccggtccgcg aatctctaag tctttctgtg caatcacaaa accgtcgttg ctgtcgcgca
    11461 gcacttgcag ccgaatttgc gcggttttgg agagcggcgt tttgtagagc agcacgcagt
    11521 gagaagccac cgcaccacga cctacacgcc cgcgaagctg gtgtaactgc gccagaccca
    11581 gacgctcagg gttttcgata atcatcaggc tggcgttagg cacatcaaca ccgacttcaa
    11641 taacggtagt ggcaactagc agatgtagct caccttgttt aaacgacgcc atcaccgcct
    11701 gtttctcggc tggtttcatc cgcccgtgta ccaggccaac attcaattct ggtagcgcca
    11761 gtttcaactc ttcccaggta gcttccgccg cctgcgcttc cagtaactct gactcttcaa
    11821 tcaacgtaca aacccagtac gcctgacgac cttcagttat gcaggcgtgg cgcacgcggt
    11881 caatgatgtc ggtacggcgg gtatcaggaa tagcgaccgt agtcactggc gtgcggcctg
    11941 gcggcagctc atctatcacc gaggtatcga gatcggcata cgccgtcatt gccagcgtgc
    12001 gggggatcgg cgtggcggtc atgatcaact gatgcggatg gaagccttgc tgctggcctt
    12061 tctcccataa cgccagacgc tgatgcacgc caaagcgatg ttgttcgtcg ataatcacca
    12121 gcgccagacc gttaaactgc acctgttcct ggaagatggc gtgcgtacca acaatcatct
    12181 gcacctgacc gctggcgatg gcttcctgct gagacagtcg cgctttgcct ttctgcttac
    12241 cggcgagcca gcccacttcg ataccgagcg gttcaaacca gttgcggaag ttattggcgt
    12301 gctgttcggc gagtaattcg gttggtgcca tcaatgccac ctgtttgccg tgggcaatcg
    12361 cacgcagcgc ggcgagggcg gcgaccagcg ttttaccgga acctacatcg ccctgtacca
    12421 ggcgcatcat cggcacatcc agcgccatat cgcgctcgat ctccgccact acgcgtgcct
    12481 gtgcgcccgt tggcttgaac ggtaaggctg cgaggagttt atttttcagc gcatcattgg
    12541 cgctcagcgg ctgggcatga aaacgctgtg ctccggcacg taaggctaac atgctgaggt
    12601 tgtgcgccag cagttcttcc agaatcagac gacgttgcgc tggatgctgc ccggtttcca
    12661 gatcgctaag ctgtagcgtc ggtggcgggc ggtgcaaagt gcgcaacgct tccggtagcg
    12721 tcatcattcc ttgtgacagt tccggcggca ggagttcttc aatggcgcag gtgtcgagca
    12781 gatccagcgc ctggtcggtt aatttacgca gcgtggcctg ctttacgcct tccgttgttg
    12841 gataaaccgg cgtgagcgtt tcctgtaatt ctggcgtgct gagatcgccc tgcacgcggt
    12901 attccgggtg gatcatctcc gcaccatatt taccgcgctt tgcttcgcca taagccagta
    12961 cacggcggcc cgtcgccagg ctatttttca ttgccgcgct gaaattgaaa aagcgcatgg
    13021 tgaggatgcc ggaaccgtcg ctgatctggc aggtcatcat ccgccgaccg ccgaaggaga
    13081 tattgcagtt cagcacttcg ccttccaccg tggcataaac gcccggcagt agttctccga
    13141 tggggtagag atgggtgcga tcttcgtagc gcaggggaag gtgtaagagt agatcctgca
    13201 cggtatgcag gttgattttc gccagtttgt tactaagtgc tgcgccaacg cccgttaggg
    13261 aactgagtgg gacagcatct aacaggcgac ctgtcatggc acttaccctg cagcctgcat
    13321 agtagcccac cagtcggcat cagcttcgat ctcgccttgc tgattgacgt ggggataagg
    13381 caggcctttg cgttttgcga ctttcgccag caccggatag ccgccttcaa acaacaggcg
    13441 ttgttgctct gcttccggca acatgctgtt ttcacgcagg tacatgcctg cattttgccg
    13501 ctgacgctgg gcttcgtaaa gaatgagggc tgaggcaacg gaaacattca gcgactgcac
    13561 catgccgatc atcggaatga tgatgtcctg atccgccagg gccaatgctt cctgcgtgat
    13621 gcccgttttc tcctgtccca tcaaaatgca ggtcgggcga gtgtaatcaa tttcgcggaa
    13681 atcgacagcg ttatcagaaa gatgggttgc cagaatctgc atgccctggc ctttgagatg
    13741 agcgacggca tcgccaatgg tgcggtgtgt tttcacctgt acccagctgt tactacccgc
    13801 cgctgccgaa gccatggtgc gcatgcggct accaggccag acggcgtgaa cttcatgtac
    13861 gccaacggca tctgcggtac gaataatcgc agaaacgtta tgaggtttgt ggacctgctc
    13921 catgcagacg gtcagatcag gctgccgcct ggcgagcatt tcgcagatgc gtgcataacg
    13981 tgttgggttc ataaaacatt aatttcggtt tcgggtgact ttaatcacgt ctggcatcac
    14041 gcggattttg cgcatgatat tcgccagatg cacacggtca cgagcggtca gacgaataaa
    14101 ggcgctgtag acgcgaccat ctttctcttc cgtattcaaa ctttgaatat tcgaggtcgt
    14161 ggtgttaatt gccgccgtca ggtttgccag cgcaccctga tgattgaaca tctccacctt
    14221 gatttcggtg atgaactcct gcgccgtctc tttatcccat tccacagcca taaacttctc
    14281 tggctctttc tggtagccac ggatgttacg gcaggattca tggtggatca ccagaccttt
    14341 accggggctg acgtgggcga taatcgggtc gccaggaatg gggcggcagc atttcgcaaa
    14401 ggtgatcagc acgccatcag cgcctttgat cggcagatgc ccgtggcttt gagtcgccgg
    14461 tggaatggag gcgtccccat gttgtagatt tttcgcgacc accacgctca ttgcgttacc
    14521 aaggccgatt tctgccagca gatcgtcaag cgttgccagc ttcatgcgat ccagctcgcg
    14581 ctgaatattt tcctgcggga tttcattgag cttacggcta ccacccaaag catggttgag
    14641 cagacgacgg cccaggctta cagaatcatc acgcttgagg tttttcagca actgacgaat
    14701 tttggcgcgc gctttcgagc taacgacaaa gttcagccaa gcggcattcg ggcgagcgcc
    14761 cggagcggta atgatttcaa cggtctgacc gctagtaagc ggctgcgaca gcgggtaagg
    14821 ctggcggtcg acgcgtgcgc ccacgcaggc atggccgata tcggtatgca ctgcataagc
    14881 gaagtcgacg ggcgttgcac cggcaggcag ctcgacaatg cgcccttccg gtgtgaaaac
    14941 gtaaatctca tccgggaaga gatcggattt aacgctctcg ataaattcaa acgaactacc
    15001 ggcgctctgt tgcagctcca gcaggctttg catccagcgc tgggcgcgga tttgtgcggt
    15061 agtactggtt tcgccgtgct ctttataagc ccagtgcgcg gcaacaccca tctccgccat
    15121 ctgatccata tcttcggtac ggatctggac ctcaaccggc acgccgtgcg ggccaatcat
    15181 cgaggtgtgc aacgactgat agccgttcgc ttttggaatg gcgatatagt ctttcacgcg
    15241 gcccggacgc ggcttgtaca ggctgtgcat ctggcccagc acgcgataac aggtgtcaga
    15301 atcattgacg atcacgcgga aagcgtagat gtccatgatt gagtgaaaac gctgctcttt
    15361 gagcaccatt ttacagtaaa tcgaataaag atgcttttcg cgaccactga cgcggcacgg
    15421 tattcccgct tcctgcaaac gcccttcgat ttcagaaaga atcttctgga tcatctcttt
    15481 acggttgccg cgcgcggctt tcaccacttc tttgattacg cgataacggt tgggatacag
    15541 cgcctcaaaa cccagctctt cgagttcggt tttaatgtgg tggataccta aacggtgcgc
    15601 cagcgggctg taaatttcga gagtttcacg ggcgatgcgg cgacgtttgt ccgggcgaag
    15661 tgagcccagc gtgcgcatgt tgtgggtacg gtcggcaagt ttgatgagga tgacgcggat
    15721 atcctgcacc atcgccataa tcatcttgcg aaagttttcg gcctgcgcct ctttcttatc
    15781 gcggaacttg agtttatcaa gtttcgacac cccctctacc agctcggcga cgcttttacc
    15841 aaaaagctgt tccatatcct ggtaggtggc gggagtatct tcaatcacgt catgcagcag
    15901 cgccgccatc agcgtttcat agtcgagttt catctcggcc agaatgcagg caaccgctac
    15961 cgggtgcgtg atatagggtt caccgcttga acgtgtttgc ccctcgtgag catcacgtgc
    16021 aacgagatac gcctgccgca gacgcttgat ttggtcttcc ggcaggtagg tttgaatcag
    16081 ttgattcagg ctttcaaaca gatacaaggg cgacccgctt tgtgattaac gacgaccttc
    16141 agcaatagcg gtaacggctt gtaattcagc ggcttcctgc tcttgctgtt cctggcgttc
    16201 gcgaacgtcg aggatctggt tgttgatcag accttcttcg atttcgcgca gcgcgattac
    16261 agtggtttta tcgttttctt ccggtaccag cggatccttt ccgcctacct gcatctgacg
    16321 agcgcgacgc gcggcgacca gtaccaggtc aaaacggtta ccaattttct ctacagcgtc
    16381 ctgaacagtt acgcgtgcca tacttaaaaa gctccacagg tgaagaaatg actgggcatg
    16441 atactgaaat caggttcagt ctgccaacaa tttgctgatt aaagcgtcat gacgctgctt
    16501 ttggcggctc atgcgcagac gttcggcgcg aataatggtc ttcaaatcgg tcaacgcggt
    16561 atcgaagtca tcattcacaa tcaggtaatc atattcggcg taatggctca tttctgcaac
    16621 agcttgcgcc atacgctttg caatgacctc ttcgctgtcc tgatcgcgac cgcgtagacg
    16681 gcggtccagt tcaattttag acggcggtaa aataaagata ctccgcgcgt gcggcatctt
    16741 ctggcgaatt tgctgcgcgc cctgccagtc gatatcgaga aaaacatcga caccggtcgc
    16801 cagtacttgc tcaatggcct cacgcgaagt gccatagtaa ttaccaaaaa cttctgcgtg
    16861 ttcgaggaac gcatctctgc taatcatttc tttaaattca tcatgattaa caaagaaata
    16921 atgttcaccg tggacttcac caggacgcgg ttggcgtgtg gtgtgtgaaa cagaaacctg
    16981 ggtgtcatac aacggttggg tttttaataa agcctgaatc aggctggatt tacccgcgcc
    17041 actgggggca gaaacaatat aaagcgtgcc ttgagccatg agtatctttc gtatgtgatt
    17101 agcgaaataa agcctacata cgagcttatt atacacggcg cggccatgtg acgtagccct
    17161 tgctcacact ttttgcgcaa agttgttgcg ttttgcgtag ctgtttccgc atttgcctgt
    17221 gatttactgc aataaagacg attctcagga agcgtcctcg cagactccgt ttttgccact
    17281 cggctttgcc ttcaccacgt tgtatatctg ccagcctgac tacagcggag gtgaagatga
    17341 aagtatggat ggcgatatta ataagtatct tgtgctggca atcatctgtg tgggcggtct
    17401 gtccggcctg gtcgccagcc agagcacagg aagaaatttt ccgcctgcaa cagcaaataa
    17461 aacagtggga cgatgactac tggaaggaag gaaaaagtga ggtggaagac ggtgtttacg
    17521 atcagttaag cgcccgtctt acgcagtggc aacgctgctt tgtgagcgag ccccgcgacg
    17581 tgatgatgcc gcctttaaat ggtgcggtta tgcatccggt tgcccatacg ggtgtacgca
    17641 aaatggcgga taaaaacgca ttaagtctgt ggatgcgaga acgtagcgat ctttgggtgc
    17701 agccaaaagt tgatggcgtt gcggtaaccc tggtttatcg ggacgggaaa ctgaacaaag
    17761 caatcagtcg cggtaatggc ctgaaaggcg aggactggac gcagaaagtt agcttaattt
    17821 ccgctgtgcc gcaaaccgtt agcgggcctt tagccaacag tacgcttcag ggggaaatat
    17881 ttctccagcg cgaggggcat atccaacaac aaatgggggg aataaatgcc cgcgcaaaag
    17941 ttgctggcct gatgatgcgc caggacgata gcgacacgct gaattctctg ggcgtttttg
    18001 tctgggcatg gccggatgga ccgcagttaa tgaccgatcg tttaaaggag ctggctaccg
    18061 caggttttac tctaacgcag aggtataccc gtgcggttaa aaatgctgat gaggttgcgc
    18121 gcgtacgcaa tgagtggtgg aaagcgaaat tacccttcgt caccgatggc gtggttgtgc
    18181 gaggggcgaa agaacccgaa tcccgccatt ggctaccagg ccaggcagaa tggctggtgg
    18241 cctggaaata tcaacctgta gctcaggttg ccaaagtgaa ggcgattcag tttgcggtgg
    18301 gtaagagcgg taaaatatcg gtggttgcgt cactcgcacc tgtcatgctg gatgataaaa
    18361 aagtccagcg ggtgaatatt ggttccgtca ggcgctggca ggagtgggat attgcgcctg
    18421 gtgatcagat cctcgtcagc cttgccggtc agggtattcc tcgcattgat gatgtggtct
    18481 ggcgcggtgc agaacgtaca aaaccgacac caccagaaaa ccgctttaac tcgttgacct
    18541 gctactttgc ttctgatgtt tgtcaggaac agttcatttc acgcttagtc tggctggggt
    18601 caaaacaggt tctggggctg gatggcattg gtgaggccgg ctggcgcgcg ctgcatcaga
    18661 ctcatcgctt tgagcatatc ttttcctggc ttttattaac gccagagcaa ttacagaaca
    18721 cgccggggat cgcgaaaagt aaaagtgcgc agctatggca tcggtttaat ctggctcgta
    18781 agcagccttt tactcgctgg gtgatggcaa tgggaatacc gctaacccgg gcggcgctta
    18841 atgccagtga tgaacggtcc tggtcgcaac ttttatttag cacggagcag ttctggcagc
    18901 agctgccggg gactggatcg gggcgcgcca gacaggttat tgaatggaag gaaaatgcgc
    18961 aaatcaagaa gctgggcagt tggctggctg cccagcagat cacaggtttt gaaccttagt
    19021 gtccttcgtg gctgtagtaa aagaccggta atcccagctt aaggcgtagc gccagtaagc
    19081 gggcaaaaaa gccgaatacc agggtggaga taattaccac atcatggtta gaaacatagt
    19141 gttgcagcgc gatgtacagc acggcggagg caaatgagac accggcatac aactctttct
    19201 ggaataccag tgggatacgt ttacagaaca tatcgcgcaa aacgccgcca aacacgccgg
    19261 tggttaccgc cgcgacaacg gcaataattg ggccgtgccc catatccagt gcaacctgtg
    19321 cgccgatgat agaaaagacc accagtccaa gcgcatcgag caccagaaac actttgcgca
    19381 ggtaaggcat tacaggagca acgatagtgg tgagtactgc ggcggtagcg acgataatca
    19441 catattccgg gtgtttgacc cagccgagcg gatagtggcc cagcagaata tcgcgcactg
    19501 accctccgcc aattgcggtg gcggtcgcaa taataattac gccaaatgta tccatgcggc
    19561 gtcgcccggc cgccagagcc cccgtcatgg cttcggcagt aatgccaacc aaatacaaaa
    19621 tgtgtaacag catggttctc tccctaaaaa tctgtctggc agggtagcga tttgtgcgta
    19681 ttgtcacgat tgagattttt taaggtgaga ggaaattgat aacttgtagt tatgtgaaat
    19741 cgctattttc tgtagcgtag atgcattctt ctgacttctt gtctgagaaa atgcataaga
    19801 aaaaataggt taaaattgag gggtggaaaa gaaaggtgca atgtttagcc attgtgttca
    19861 ccgcacctgg actttggctt acgcatcggg tatctcaatc aacaaaaaga gtttttagtt
    19921 tctgctattt cttctctgta atttttacac tgttttccaa ctgcttgatg ctgacctgcg
    19981 gggtcggcaa ctcctcaggc atggttccgc caagttcctg aatggtttgc ctcaccttgc
    20041 tacccacatc aaagtgtgtg gtattagctt gctgttttga attcacgcca tcccgcttga
    20101 gtttttcttc tgtttgggta gctcgaaaga gattagccgc cagttctgtt gaacccatat
    20161 gatccaggat cttctgactc tttttcagcc ccttcagttg gtggatggct ttctgatcta
    20221 atccaccata cagcccctgg taaccatgat tctgaaatat ggcgaaatca gtagctgtcg
    20281 ctaccgcagc ttgctgtgct gcctcaacca attgtttgtt atgttctttc aactcatttc
    20341 tcagaaacag acgcttttcg tcttcacgaa gttgcttaaa tgcttcatcg tcagcaagct
    20401 cctgccgtcg ggtctggata gcaaaataag tctgccctgc cgcaatgacc ggtttcgcag
    20461 ggtcgccgtt ttgtactacc aggtaacagg catagcggga gagatgaaca tcttcaagtt
    20521 ctcgttgagc ccctgaacct aatacaacca ttttggtggt ttcaacgaaa tggtcagatg
    20581 cagcttgatt gctggcttcg cacgcttgag tcgcgcgggc cagtaccttt tgaaaattgc
    20641 gccagtcccg ataatccagt aacggtgcca attctctagc tgaccaaaat tcttgccctt
    20701 cagtgccata atgccgtatt tcttcaaaag gttgatgatg ttcgttcatt gcccactcca
    20761 tgtgatatcg attgcaaaga aagtaaatct gtaactgtat ttatatacag ttgtgagtat
    20821 ctcacgttct cagtgtgctg ggcaaaaaaa catcacaaaa tctggagcag gcacgcaaaa
    20881 cagaattgaa gttgcgggga agaaaaatgc cgctctgttt gagtgggcgg catttgtgaa
    20941 ggctgaaaag taagaattgg cgttacgagt tattcaatgt tctgaatctg ctcgcgcatc
    21001 tgctcaatca acactttcag ctcaatcgcg gagtttgtca cttcggcatt gatagacttc
    21061 gacgcaagag tgttcgactc gcggttgaat tcctgcatca taaagtccag acgacgacca
    21121 acggcttctt ttttcttcag aatgttgtag gtctctttga catgcgcttc caggcggtcc
    21181 agttcttcgg caacgtcaat tcgttgtgcc agcagaacca gctcctgctc cagacggttg
    21241 ttttccaact gaacctgagc atcttccagc ttcgcgacca gacgctcacg ctgccattgc
    21301 aggatttccg gcatatgggc gcggactttg accacttcgg cggtgacgcc ttccagacgc
    21361 tgcacgatca atgctttcag tgcctgacct tcggtttcgc gcgcgacaat aaagtcgtcc
    21421 agcgtaccat ccagcgccgc gagaatttca gcggcaatgg cgtcaagatc ctgctcctgg
    21481 gctgccatca cgcccggcca gcgtagaata tcaaccgggt tgatttcccc ttcgtcactc
    21541 tgcattttta cccagttcgc ggcagttacc agctgtttag ccagtttttc gttgaggatc
    21601 agctcacctt gcgcgctaac atctggctca tagcgcaggg tacattccac tttaccgcgc
    21661 gtcaggcgag aacgaatacg ctcgcgaacg acaggttcaa ggctacggaa ctgctccggc
    21721 agacgaaagt aagtttccag ataacgctgg tttaccgagc gcatttccca ggttgcgctc
    21781 ccccattcac ccttgatttc acgccgggcg taggcggtca tactgcggat catagacgtt
    21841 cctgtttata aaaggagagg tggaaggatt atagccatcg atgccttgtc aggataggaa
    21901 taaccgccgg aagtccgtat aatgcgcagc cacatttgtt tcaagccgga gatttcaata
    21961 tgcgtccagc aggccgtagc aataatcagg tgcgtcccgt taccctgact cgtaactata
    22021 caaaacatgc agaaggctcg gtgctggtcg aatttggcga taccaaagtg ttgtgtaccg
    22081 cctctattga agaaggcgtg ccgcgcttcc tgaaaggtca gggccagggc tggatcaccg
    22141 cagagtacgg catgctgcca cgttctaccc acacccgtaa cgctcgtgaa gcggcgaaag
    22201 gtaagcaggg tggacgcaca atggaaatcc agcgtctgat cgcccgtgct cttcgcgcgg
    22261 cagtagattt gaaagcgctg ggtgagttca ccattacgct ggactgcgac gtgcttcagg
    22321 ctgatggtgg cacgcgtacc gcgtcgatta cgggtgcctg cgtggcgctg gcagatgcgc
    22381 tacagaagct ggtggaaaac ggcaagctga aaaccaaccc gatgaaaggg atggtagccg
    22441 cagtttctgt cggaattgtg aacggcgaag cggtttgcga tctggaatac gttgaagact
    22501 ctgccgcaga gaccgacatg aacgtagtga tgaccgaaga cgggcgcatc attgaagtgc
    22561 aggggacggc agaaggcgag ccgttcaccc atgaagagct actcaccttg ttggctctgg
    22621 cccgaggggg aatcgaatcc attgtagcga cgcagaaggc ggcgctggca aactgatttt
    22681 taaggcgact gatgagtcgc cttttttttg tctgtagaaa agtaagatga ggagcgaagg
    22741 catgaaacca tatcagcgcc agtttattga atttgcgctt ggcaagcagg tgttaaagtt
    22801 tggcgagttt acgttgaagt ccgggcgcaa aagcccctat ttcttcaacg ccgggctgtt
    22861 taataccggg cgcgatctgg cactgttagg ccgtttttac gctgaagcgt tggtggattc
    22921 cggccttgag ttcgatctgc tgtttggccc tgcttacaaa gggatcccga ttgccaccac
    22981 taccgccgtg gcgctggcgg agcatcatga ccttgacctg ccgtactgct ttaaccgcaa
    23041 agaggcaaaa gaccacggtg aaggcggcaa tctggttggt agcgcgttac aaggacgcgt
    23101 aatgctggta gatgatgtga tcaccgccgg aacggcgatt cgcgaatcga tggagattat
    23161 tcaggctaat ggcgcgacgc ttgctggcgt gttgatttcg cttgatcgtc aggaacgggg
    23221 gcgcggcgag atttcggcga ttcaggaagt tgagcgtgat tacaactgca aagtgatctc
    23281 tatcatcacc ctgaaagacc tgattgctta cctggaagag aagccggaaa tggcggaaca
    23341 tctggcggcg gttaaggcct atcgcgaaga gtttggcgtt taaagaaact cgccggatga
    23401 aaagtcatcc ggcgtcatat tactgcaact gtgccgcaat tagcggccag cgggcgtcaa
    23461 aatcatccgt cgggcggtat ttaaattcgc tgcggacaaa acgtgacagc ataccttcac
    23521 agaaggctag gatctggctt gccagcaggg tttcatcggt ggtgtaacct tcaccctcac
    23581 gcattctctt ttcacgcaat acctgacgca gctgcgcttc aatacgctcg aacagctggt
    23641 tgatgcgccc ttgcaggcga tcctgttcaa acattagcgc atgaccagtg aggatgcggg
    23701 tcaggccagg attacgctca ccaaaaccga gaagcagcaa cacaatcaga cgcaggcgcg
    23761 ctgtggtgtc tttctcatct ttcagaatca gattgatgcg agtaatcagg ctatcttcga
    23821 taaactcaat caggctatcg aacatgcggg tcttactggg gaagtggcga tacagtgccg
    23881 cttcggaaac gccgacagag gcggccagtt ttgccgtcgt gatacgttgg cttccatcgc
    23941 tggattccag catcagcgcc agagactgaa gtatttcctc gcgacggttc cttttcgcag
    24001 tttgtttttc tgccatgtta caaaataccc ctgaaaataa gcacttgcca ggcggcaccc
    24061 acgctatgac cgcaaacgaa atgtttacgg ctatgttatg acgttattcg gatgcgtatg
    24121 tgttactgac gaccagagtg accaaagccg ccttcaccgc ggtcggtggc gtcgaaatct
    24181 tccaccagat taaattcagc ctgtactacc ggaacaaaaa tcatctgggc gatacgttcg
    24241 ccaggttgaa tggtgaagct gtcctgacca cggttccaca cggaaatcat caactggccc
    24301 tgatagtcgg aatcgatcaa tcctaccagg ttaccaagca cgataccgtg cttatgtccc
    24361 aatccggagc gcggcagcat cattgccgcc agtgaaggat cggcaatatg aatcgccagc
    24421 ccggtcggaa ccagcgtagt gtcacccgga gccagttcta cggcgtcgtc gagacaggca
    24481 cgcaggtcaa gtccggcaga gccagaggtg gcataagtcg ggagcggaaa ttccttccca
    24541 acgcgcgggt ccagaatctt aacgtcgatt tttttcatca taacgggtca cgatctcgtc
    24601 gagtaataat tggccaagga gctctttgcg ctcaagcggt aagactttat ctccgtcctg
    24661 ccagaaaagg tgtaatgcgt tgttgtcgct gttaaatcct tgagttggct gggaaacatc
    24721 gttcgcgcag atcagatcaa ggtttttacg gatacgtttt tgccgggcgt attcttccac
    24781 attatttgtt tcggcggcaa atccaacgac gtagggtcga tggtctttta gtgcggcaac
    24841 gcctgcgacg atatcggggt ttttaaccat ttttattgtt aattcatcac cctgcgtggc
    24901 ctgctttttg attttctctg gggccacggt agctgcgcga taatccgcca cggcggcgca
    24961 gccgataaaa atattttgct gctgtacaga agcattcacg gcggcttcca tttccagcgc
    25021 ggtcatcaca tcaacacgtt taacaaacgg tggcgtcggt agtgaaaccg gacctgatac
    25081 cagcgtgacg ttcgcgccac gacgggcagc ggcggcggcg atagcaaaac ccatcttgcc
    25141 ggagctgtga ttagagatat aacgcacggg atcgagcggt tcacgcgtcg ggccggcggt
    25201 aatcataatg ttcagatgtt tcaggtcgtt gacgggcgaa aaatgcgcta ccgccatatc
    25261 cacaatggtt aacggatcga gcattcgccc aggaccgata tcaccacaag cctgactgcc
    25321 actgtctggc ccccagatga gcaaaccacg ggaagcaagc acctctaaat tatgctgcgt
    25381 ggcagcggca cggtacatct gctggttcat ggcggggagc acggctacag gcgcaggtgt
    25441 agccagacaa atcgtcgata ccaggtcatt cgccattccg gcagcaacac gggcaatcaa
    25501 atctgccgtg gcaggggcga gaatcactaa atcagcccat ttacccagct caatatggcc
    25561 catagcggct tcggctgccg ggtccagcaa actgtcggaa acgggataac cagaaaccgc
    25621 ctgcaagcta agtggggtga taaaggcttt tgccgcttcg gtcatggcta cgcggacgtc
    25681 ggccccgcga tcgcgcaaac gacgcaccag ttcaggggtt ttataggcag caataccgcc
    25741 gctaacgccg agaacgattt ttttaccggc caggctcatg atgatttttc ctgttgggtg
    25801 acaccagaag ttggcgattt tatcacaata cttttgttgt cgtgccttca cctgagattc
    25861 actttgcgag gcgctttcca ggattgaaaa ctggccgtcg atttaacgga acggctatga
    25921 caggatgcga gcaccacaaa ggaggtgaag gtgaaaaaca atgcacagct gttgatgccg
    25981 cgcgaaaaaa tgctgaagtt tggtattagc gccttaactg atgtcgagct gctggcgcta
    26041 tttctgcgta ccggaacgcg cggtaaagat gtattaaccc tggcaaaaga gatgctggag
    26101 aatttcggct ctctttatgg cttgttaacc tctgaatatg aacaatttag tggtgttcat
    26161 ggaattggcg tagcgaaatt tgcccagtta aaggggattg ctgaactggc gcgacgttac
    26221 tacaacgtgc ggatgcgtga agaaagccct ttactcagcc cggagatgac gcgggaattt
    26281 ttacaaagcc agctcacggg tgaggagcgg gagatcttta tggtgatctt tctcgactcc
    26341 caacaccggg ttataacgca tagccgtctt ttttccggca cgctaaacca tgttgaagtc
    26401 catcctcggg aaattatccg cgaagcgata aaaataaacg cctcggcgct gatccttgca
    26461 cataatcacc cttcgggttg tgctgaaccc agtaaagcgg ataaactcat tactgaacgg
    26521 ataataaaga gttgtcagtt catggattta cgcgtgctcg accatatcgt gattgggcgt
    26581 ggagagtatg tttcttttgc cgaacgcggc tggatttaac ccgctatgcg cgatccttcg
    26641 ggatctttgt ctgttcggga cttgagcaca tcgctgagtc agcgtatact acgccacctt
    26701 tgagaatctc gggtttggca tttgggcctg gcaatcgaga gttcacatag aactgcgatg
    26761 accgggctgt aaagcctgac gaggcgccaa taccccatac gaagctcgag ctaatttgat
    26821 ttttggagaa tagacatgtc ccgagtctgc caagttactg gcaagcgtcc ggtgaccggt
    26881 aacaaccgtt cccacgcact gaacgcgact aaacgccgtt tcctgccgaa cctgcactct
    26941 caccgtttct gggttgagag cgagaagcgt tttgtcaccc tgcgcgtatc tgctaaaggt
    27001 atgcgtgtaa tcgataaaaa aggcatcgat acagttctgg ctgaactgcg tgcccgtggc
    27061 gaaaagtact aagtacttag aggaaataaa tcatggctaa aggtattcgt gagaaaatca
    27121 agctggtttc ttctgctggt actggtcact tctataccac tacgaagaac aaacgtacta
    27181 agccggaaaa actggaactg aaaaaattcg atccagttgt tcgccagcac gtgatctaca
    27241 aagaagcgaa aatcaaataa ttctcgcttt gatgtaacaa aaaacctcgc tccggcgggg
    27301 ttttttgtta tctgcttgcc cccatattga ctgcatctgt tcattcctgg agatgctatg
    27361 cctgaattac ccgaagttga aaccagccgc cgcggcatag aaccgcatct cgttggtgca
    27421 accattcttc atgcagtggt gcgcaacgga cgcttgcgct ggccggtttc agaagagatc
    27481 taccgtttaa gcgaccaacc agtgcttagc gtgcagcggc gggctaaata tctgctgctg
    27541 gagctgcctg agggctggat tatcattcat ttagggatgt ctggcagcct gcgcatcctt
    27601 ccagaagaac ttccccctga aaagcatgac catgtggatt tggtgatgag caacggcaaa
    27661 gtgctgcgct acaccgatcc gcgccgcttt ggtgcctggc tgtggaccaa agagctggaa
    27721 gggcataatg tgctggccca tcttggaccg gagccgctta gcgacgattt caatggtgag
    27781 tatctgcatc agaagtgcgc gaagaaaaaa acggcgatta aaccgtggct gatggataac
    27841 aagctggtgg taggggtagg gaatatctat gccagcgaat cactgtttgc ggcggggatc
    27901 catccggatc ggctggcgtc atcactgtcg ctggcagagt gtgaattgtt agctcgggtg
    27961 attaaagcgg tgttgctgcg ttcgattgag cagggtggta caacgctgaa agattttctg
    28021 caaagtgatg gtaaaccggg ctatttcgct caggaattgc aggtttacgg gcgaaaaggt
    28081 gagccgtgtc gggtgtgcgg tacgccgatt gtggcgacta aacatgcgca gcgggcaacg
    28141 ttttattgtc ggcagtgcca gaagtaattc atgcgcgccg gatggcatac catccggcat
    28201 aaacgctacg ctaacttcgc catcagcgcc tgatggacat tctccggcag gaaatgggtg
    28261 acatcgccct gatggcgcgc cacctctttc accaacgatg aagagataaa cgaccactct
    28321 ttcgacggca tcagaaacac actttccagt tccggcatta agtggcgatt catatgcgcc
    28381 agctgcattt catattcaaa atctgccacc gcacgcaggc cacgaatcag caccgtagcg
    28441 tgttgattac gggcgaagtt cgccatcaaa tcactaaacc cgaccacttc cacgttcccc
    28501 agatgcgcgg ttgcctgctg tgccagtgcc acacgctctt ccagggtaaa catcggtttt
    28561 ttactggggc tggcggcaat cgccagaata acgtgatcga acatctgcgt ggcgcgcgtc
    28621 acgatatcga tatgaccatt ggtaatggga tcgaaagtac ccggataaat cgcccgtttt
    28681 tgcataacaa cctcaatgcg ttttcggtgg caggtaaggt tccagcagtt gaagcagacg
    28741 ctgtagcgcg ccctggtttt gatacagtac ttcaacggca tgacggccat agaaactacg
    28801 gtaatcggcg tcggtgagta aagaggaaac ctcttttgca agcgtagtgg catcggtaac
    28861 ggtaatcagc ccgcttgcct gctccagccg cgcgcaaatg tctttaaagt taaaagtatg
    28921 cggccccatc aataccggaa ttgcgtgtgc ggcagcttcc agcggattat gcccaccacg
    28981 ttcaaccagt gaaccgccaa caaaggcgag atcggcaatg ccgtacagta acatcaactc
    29041 gcccatcgta tcgccaacca caacctgcgt gctggtggag gggacttccc ctgaagagcg
    29101 tgtgatatag cttagtccag cctggcggac aaggttaatc gcatccggga agcgttctgg
    29161 atgacggggt accaggatga gcaataaatt cgggaattgc tgtaataaag cctgatgtgc
    29221 ggcaatgacc acgctctctt caccttcgtg agtgctggtg gcaatccata ccgggcggtg
    29281 tggtgcccac tggcggcgca gcgtcacggc tttagcagcc aactgcggcg ttacagaaat
    29341 atcgaatttc aggctaccgg taacggttac ctggttattt ttcgcgccca gcgccacaaa
    29401 acgtgcacca tcttcttcat tttgggcggc aatcagcgta atacgacgca gcaagcgacg
    29461 gacgaattta cccagtttgg cataacctgc ggccgagcgg gcagagagtc gcgcgttagc
    29521 gatcaccagc ggaattttac gtttatgtag cgccgcaatc aggttaggcc atagttcggt
    29581 ttccataatc aacaccagtt tagggtcgac tttattcagg aaacggttga gtgcatcggg
    29641 cagatcatac ggcagataaa cgtgctgaac atccttcccg aaagccgatt gtacgcgctc
    29701 cgaaccggtt ggcgtcatgg ttgttacggt aataggtaaa tcaggataac gatgacgcag
    29761 cgcgcgcacc aacgggatcg ccgccagagt ttcaccgacg gagacggagt gcagcataat
    29821 gccgcctggt tttagcggat ggcggtaaaa accgtaacgt tcaccccagc gttttcgata
    29881 ggccggagcc ttacgtccgc gcacccagag ccgtatccag atcagcggct gaataaggta
    29941 gagaagggcg gtgtaaagca attcgagcat agtaaatagc tgacttatgg atgtgctggg
    30001 gattctatgt atttagctgt ggctttacca ttacttttcc cgtttttgac ttaaatagca
    30061 atttacccgc caattcttgc tgaacatcta tacttttttt gtatttttat gcgattcatt
    30121 gatttctgct gtacgtgcag gaaggaatcg cagagcaaca gtgaaagaat atggttgcta
    30181 atgattttat ttgattgatt tctttattga aaatcaacaa tgtgcttaaa aatcattcgg
    30241 gtcggattgc cgcgaaagaa gtgatacact agcgcgtcaa tatcagtgcg gtatcagtat
    30301 tcaggtagct gttgagcctg gggcggtagc gtgctttttc tgcttcacct taatcggata
    30361 atctcaacaa aaagagttac ttgtggataa gccatttcga agaattttgc tcattaagat
    30421 gcgttttcat ggggatatgt tattaactac tcccgtcatt agttcgctga aaaaaaatta
    30481 ccctgacgca aaaatcgatg tgctgcttta tcaggacacc atcccgatcc tgtctgaaaa
    30541 tccagagatt aacgcgctct acggcataaa aaataaaaaa gcaaaagcct cagaaaaaat
    30601 tgccaacttt tttcatctca tcaaggtatt acgtgccaat aagtatgacc ttatcgtcaa
    30661 tcttaccgat caatggatgg ttgctatact ggttcgctta ttaaatgccc gtgtgaaaat
    30721 ttcccaggat tatcatcatc ggcagtctgc tttttggcgt aaaagtttca cccatttggt
    30781 gccgttgcag ggtggaaatg tggtggaaag taacttatcc gtgctgacac cattgggact
    30841 tgattcgttg gtgaagcaga caaccatgag ttacccgcct gcaagctgga aacgtatgcg
    30901 tcgcgaactt gatcacgctg gtgttggaca aaattatgtg gttatccaac ctacggcgcg
    30961 gcaaatcttc aaatgctggg acaacgccaa gttttccgct gtgattgatg ccttacatgc
    31021 tcgtggttat gaagtcgttc tgacgtccgg cccagataaa gacgatctgg cctgcgtcaa
    31081 tgaaattgcg caaggatgcc agacgccacc agtaacggcg ctggctggaa aggtgacctt
    31141 cccggaactt ggtgcgttaa tcgatcatgc gcagctgttt attggcgttg attccgcacc
    31201 ggcgcatatt gccgctgcag ttaatacgcc gctgatatcg ctgtttggtg cgacagacca
    31261 tattttctgg cgtccttggt caaataacat gattcaattc tgggcgggag attaccggga
    31321 aatgccaacg cgcgatcagc gtgaccgaaa tgagatgtat ctttcggtta ttccggcggc
    31381 agatgtcatt gctgctgtcg ataaattact gccctcctcc acgacaggta cgtcgttatg
    31441 atcgttgctt tttgtttata taaatatttt ccctttggcg gtttgcagcg cgattttatg
    31501 cgtattgctc agacagtcgc cgcccgaggt catcatgttc gggtttatac ccagtcgtgg
    31561 gaaggcgaat gccctgatgt atttgaactg atcaaagtgc cggttaaatc gcataccaat
    31621 cacgggcgca atgcggagta ttttgcctgg gtgcaaaaac atttacgcga acatcccgtc
    31681 gataaagtcg ttggattcaa caaaatgccg gggctggacg tttattatgc cgctgatgtt
    31741 tgttatgccg agaaagtcgc gcaggaaaaa ggctttttct atcacctgac gtcacgttat
    31801 cgccattatg ccgcctttga gcgggcaacc ttcgaacagg gcaagccgac acagctgctg
    31861 atgctgacag ataagcaaat cgccgatttc cagaaacatt atcagactga agcggagcgt
    31921 tttcatattc taccaccggg gatttatcct gatcgtaaat atagccagca gccagctaat
    31981 agccgtgaaa tcttccgtaa gaagaatgga ataaccgaac aacaatattt attgttgcag
    32041 gtcggttcag acttcacgcg taaaggtgtc gatcgttcca ttgaagcact tgcttcgtta
    32101 ccggattcgc tgcgccacaa cacattgcta tatgttgttg gacaggataa accgcgaaaa
    32161 tttgaggcgc tggcagaaaa acgcggcgtg cgcagtaatg ttcacttctt ctcggggcgc
    32221 aacgatgtct cggaattaat ggcggcggcg gatttattac tgcatcctgc ctaccaggaa
    32281 gcagcgggaa ttgtgctact ggaagcgatt actgcaggat taccggtact aacaacagcc
    32341 gtttgtggct atgcgcatta tattgtcgac gccaattgcg gcgaggctat tgctgagcca
    32401 ttccgccagg aaacattgaa tgagatttta cgcaaagcgt taacgcaatc ttcattgcgc
    32461 caggcctggg cggaaaatgc gcgacattat gctgatacac aagatttata cagtctgcca
    32521 gagaaagcgg cggatatcat aacgggtggt ctggatggtt gaacttaaag agccgtttgc
    32581 cacgttatgg cgcggtaaag atccttttga ggaagttaaa accttgcagg gtgaggtttt
    32641 tcgtgaactg gaaactcgcc gcactctgcg atttgaaatg gcgggcaaaa gctattttct
    32701 caaatggcat cgcggcacga ccctgaaaga gataatcaaa aatttactct cattgcggat
    32761 gccagtatta ggcgcagacc gcgaatggaa tgcgattcat cgactgcggg atgtcggcgt
    32821 tgatactatg tatggggtgg catttggcga aaaaggcatg aatccgctga ccagaacttc
    32881 atttattatt accgaagatc tgacaccaac cataagtctt gaagattact gtgctgactg
    32941 ggcgactaac cctccagatg ttcgcgtaaa gcgtatgctt attaagcgtg tcgcgacgat
    33001 ggtgcacgat atgcatgctg cgggcattaa ccaccgtgac tgttatatct gtcatttcct
    33061 gctgcacttg cctttttccg gtaaggaaga ggagttaaaa atttcggtaa ttgacctgca
    33121 ccgggcgcag cttcgcacgc gcgttccacg tcgttggcgg gataaagatc ttattgggct
    33181 ttatttttct tcgatgaata tcggcctgac tcagcgggat atctggcggt ttatgaaagt
    33241 gtattttgcc gccccgctta aagacattct caagcaggaa caaggactgc tgtcgcaagc
    33301 agaagcaaaa gccacaaaaa tcagggaaag aacgattcga aaatcgttgt aacccgtaac
    33361 ttatttttgc caaaattttg gatacagaat aaatatgtct caactcaatg atagtgacat
    33421 catccttttt gagtataatt ttcattatca aaatataaga tctaaaaata ctcttgatat
    33481 agcatttggt attgacagaa attttctttt tggatgtggt gtagccatcg catctattct
    33541 attaaacaat agagaaatct cttgtgaatt t
//
LOCUS       DAZFBR010000046        33412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFBR010000046 DAZFBR010000000
VERSION     DAZFBR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.046"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            1..2220
                     /locus_tag="ACHJZ2_002371"
                     /pseudo
     CDS             1..2220
                     /locus_tag="ACHJZ2_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            2557..3534
                     /gene="glaH"
                     /locus_tag="ACHJZ2_002372"
     CDS             2557..3534
                     /gene="glaH"
                     /locus_tag="ACHJZ2_002372"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO4568462.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3554..4822
                     /gene="lhgO"
                     /locus_tag="ACHJZ2_002373"
     CDS             3554..4822
                     /gene="lhgO"
                     /locus_tag="ACHJZ2_002373"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO4568463.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4845..6293
                     /gene="gabD"
                     /locus_tag="ACHJZ2_002374"
     CDS             4845..6293
                     /gene="gabD"
                     /locus_tag="ACHJZ2_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4568464.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6307..7587
                     /gene="gabT"
                     /locus_tag="ACHJZ2_002375"
     CDS             6307..7587
                     /gene="gabT"
                     /locus_tag="ACHJZ2_002375"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO4568465.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7825..9225
                     /gene="gabP"
                     /locus_tag="ACHJZ2_002376"
     CDS             7825..9225
                     /gene="gabP"
                     /locus_tag="ACHJZ2_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO4568466.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9246..9908
                     /gene="csiR"
                     /locus_tag="ACHJZ2_002377"
     CDS             9246..9908
                     /gene="csiR"
                     /locus_tag="ACHJZ2_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO4568467.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9909..10358)
                     /gene="kbp"
                     /locus_tag="ACHJZ2_002378"
     CDS             complement(9909..10358)
                     /gene="kbp"
                     /locus_tag="ACHJZ2_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO4568468.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10442..10600)
                     /gene="yqaE"
                     /locus_tag="ACHJZ2_002379"
     CDS             complement(10442..10600)
                     /gene="yqaE"
                     /locus_tag="ACHJZ2_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO4568469.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10783..11082
                     /gene="ygaV"
                     /locus_tag="ACHJZ2_002380"
     CDS             10783..11082
                     /gene="ygaV"
                     /locus_tag="ACHJZ2_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO4568470.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            11092..11610
                     /gene="ygaP"
                     /locus_tag="ACHJZ2_002381"
     CDS             11092..11610
                     /gene="ygaP"
                     /locus_tag="ACHJZ2_002381"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO4568471.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(11663..12067)
                     /gene="stpA"
                     /locus_tag="ACHJZ2_002382"
     CDS             complement(11663..12067)
                     /gene="stpA"
                     /locus_tag="ACHJZ2_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO4568472.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            12735..13184
                     /gene="alaE"
                     /locus_tag="ACHJZ2_002383"
     CDS             12735..13184
                     /gene="alaE"
                     /locus_tag="ACHJZ2_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO4568473.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(13221..13565)
                     /gene="ygaC"
                     /locus_tag="ACHJZ2_002384"
     CDS             complement(13221..13565)
                     /gene="ygaC"
                     /locus_tag="ACHJZ2_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO4568474.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            13717..14046
                     /gene="ygaM"
                     /locus_tag="ACHJZ2_002385"
     CDS             13717..14046
                     /gene="ygaM"
                     /locus_tag="ACHJZ2_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO4568475.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(14196..15530)
                     /locus_tag="ACHJZ2_002386"
     CDS             complement(14196..15530)
                     /locus_tag="ACHJZ2_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4568476.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            15618..16049
                     /locus_tag="ACHJZ2_002387"
     CDS             15618..16049
                     /locus_tag="ACHJZ2_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO4568477.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            16258..16503
                     /gene="nrdH"
                     /locus_tag="ACHJZ2_002388"
     CDS             16258..16503
                     /gene="nrdH"
                     /locus_tag="ACHJZ2_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO4568478.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            16500..16910
                     /gene="nrdI"
                     /locus_tag="ACHJZ2_002389"
     CDS             16500..16910
                     /gene="nrdI"
                     /locus_tag="ACHJZ2_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO4568479.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            16883..19027
                     /gene="nrdE"
                     /locus_tag="ACHJZ2_002390"
     CDS             16883..19027
                     /gene="nrdE"
                     /locus_tag="ACHJZ2_002390"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4568480.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            19037..19996
                     /gene="nrdF"
                     /locus_tag="ACHJZ2_002391"
     CDS             19037..19996
                     /gene="nrdF"
                     /locus_tag="ACHJZ2_002391"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4568481.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
     gene            20352..21554
                     /gene="proV"
                     /locus_tag="ACHJZ2_002392"
     CDS             20352..21554
                     /gene="proV"
                     /locus_tag="ACHJZ2_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO4568482.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPNELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEIINNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            21547..22611
                     /gene="proW"
                     /locus_tag="ACHJZ2_002393"
     CDS             21547..22611
                     /gene="proW"
                     /locus_tag="ACHJZ2_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO4568483.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            22668..23660
                     /gene="proX"
                     /locus_tag="ACHJZ2_002394"
     CDS             22668..23660
                     /gene="proX"
                     /locus_tag="ACHJZ2_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO4568484.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            23852..25036
                     /locus_tag="ACHJZ2_002395"
     CDS             23852..25036
                     /locus_tag="ACHJZ2_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4568485.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            25160..25897
                     /gene="ygaZ"
                     /locus_tag="ACHJZ2_002396"
     CDS             25160..25897
                     /gene="ygaZ"
                     /locus_tag="ACHJZ2_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO4568486.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            25887..26222
                     /gene="ygaH"
                     /locus_tag="ACHJZ2_002397"
     CDS             25887..26222
                     /gene="ygaH"
                     /locus_tag="ACHJZ2_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO4568487.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            26313..26843
                     /gene="emrR"
                     /locus_tag="ACHJZ2_002398"
     CDS             26313..26843
                     /gene="emrR"
                     /locus_tag="ACHJZ2_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO4568488.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            26970..28142
                     /gene="emrA"
                     /locus_tag="ACHJZ2_002399"
     CDS             26970..28142
                     /gene="emrA"
                     /locus_tag="ACHJZ2_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO4568489.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            28159..29697
                     /gene="emrB"
                     /locus_tag="ACHJZ2_002400"
     CDS             28159..29697
                     /gene="emrB"
                     /locus_tag="ACHJZ2_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO4568490.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(29761..30276)
                     /gene="luxS"
                     /locus_tag="ACHJZ2_002401"
     CDS             complement(29761..30276)
                     /gene="luxS"
                     /locus_tag="ACHJZ2_002401"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO4568491.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(30426..31982)
                     /gene="gshA"
                     /locus_tag="ACHJZ2_002402"
     CDS             complement(30426..31982)
                     /gene="gshA"
                     /locus_tag="ACHJZ2_002402"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO4568492.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(32055..32483)
                     /gene="yqaA"
                     /locus_tag="ACHJZ2_002403"
     CDS             complement(32055..32483)
                     /gene="yqaA"
                     /locus_tag="ACHJZ2_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO4568493.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(32480..33046)
                     /gene="yqaB"
                     /locus_tag="ACHJZ2_002404"
     CDS             complement(32480..33046)
                     /gene="yqaB"
                     /locus_tag="ACHJZ2_002404"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO4568494.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(33327..33403)
                     /locus_tag="ACHJZ2_002405"
     tRNA            complement(33327..33403)
                     /locus_tag="ACHJZ2_002405"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33367..33369),aa:Arg,seq:acg)
BASE COUNT         8144 a         8507 c         8478 g         8283 t
ORIGIN      
        1 cctatcgaca acccctcatt gttatcatgg aacattactg acggggatct aaattccaaa
       61 ttaaatacat tagaatatct aaactgtgta acaaatatta ttaatgcttg tggagtttat
      121 cctcaggatt taaaagacag agaaattata tcaacttttc acgcagaaaa agtcattaat
      181 gatctgttaa aaaacgatta taaaatttcc ctttctccag atacaactta ccgagagctg
      241 aataaagctg cacagcgtag cattacagcg ccagacagga taggagaagg gaaaatatgg
      301 gtttatcaac gagatacaat ggttgaaaga ggtgataaca gcggtgttca tcagtatggt
      361 ccagctgaac atttcactca cattatatct gacaaaccgt ccccaaaaga taagtatgtt
      421 gcatatgcta ttaacattcc tgactatgag ctagcagccg atgtatataa tattaacgtg
      481 acgtcacctt ccggacagca agaaacattt aaaatattaa tcaatccaga acatctacgg
      541 caaacacttg agcgtaaatc ccttactgct gttcagaaat cacaatgtga aatcatcacc
      601 cccaaaaaac ctggcgaagc gattcttcat gcttttaatg ccacctacca gcaaatcagg
      661 gaaaatatgt ctgagtttgc acgttgccat tatgggtata tacaaatccc tccagtgaca
      721 actttccgcg ccgacggacc agaaactccc gaagaagaaa aaggttactg gtttcacgct
      781 tatcaacccg aagatctttg taccatccat aatccaatgg gagatttgca ggattttatc
      841 gcattggtta aagatgctaa aaaatttggt atcgatatca ttcctgatta tacctttaac
      901 tttatgggaa tcgggggtag tggtaaaaat gacctggatt atccctctgc tgatatacga
      961 gcgaagatca gtaaagatat agaaggtggt atccctggct attggcaagg tcaggttttg
     1021 attccattta ctatagatcc agtaacaaaa gaacgtaaac aaatccatcc agaagatata
     1081 catctcacag caaaagactt cgaagcaagt aaagataata tttctaagga tgaatgggaa
     1141 aacctccatg cattaaaaga aaagcgttta aatggaatgc ctaaaacccc ccccaaaagt
     1201 gactaggtta ttatgttgca aaatcaatac gttcgtgaaa tgcgaaaata tggcgtacga
     1261 ggtttacgtt acgatgcagc aaaacactca aaacatgaac aaatagaaag atcaataacc
     1321 ccaccgctta aaaattataa tgagcgatta cacaatacta acttatttaa cccaaaatat
     1381 catgaaaaag ccgttatgaa ttacatggaa tatctggtaa cttgccagtt ggatgaagaa
     1441 caaatgtcat cgctgcttta tgaaagagat gatttaagcg ccattgattt ttcattgctc
     1501 atgaagacga taaaagcttt ttcatttggt ggcgatctcc aaacccttgc atcaaaaccg
     1561 agttccacaa tctcaagcat cccatcaaaa agacggatat tgattaacat taaccacgat
     1621 tttcctaaca atggcaatct tttcaatgac tttctattta accatcaaca agatgaacaa
     1681 ttagcaatgg catatatggc cgctctcccg ttcagcaggc ctttagttta ctgggatggc
     1741 caagtattaa aatcaacgac tgaaattaaa aattatgatg ggtcgacgcg tgtcggcggt
     1801 gaggcgtggc ttaataaagg ttgctctacc tatcagcagc tctacaatga attccacgca
     1861 ttatatatag ataaagcagg aatatggagc gcatttgagg gtgtatttgc aactaaaaac
     1921 gttctggcct ttagtcgtgg ggattcggtg aacattaatc actctcctca tgatggacta
     1981 gttataataa ataaaggaaa cgaagaagtt gaaggtgcct ggcctaacaa attgcaacct
     2041 ggaaaatata aaaacatggg gagtaatagc gttaacatta ttattaataa tacccgaaaa
     2101 attatccccc ctggtaaagc atttatgctt agaggcggaa ctctaaatat caatattcct
     2161 gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc tgaactatct ctatttgtaa
     2221 tttaatgtta tatctgcccc gctaaaacgg ggcagataat atatttagtt tactagcggt
     2281 cattttgcag tgaagccatt taccgttttt tatcgaccag ataatctgtt ctctgatggt
     2341 aatccccccc taacctgttg ctttagttat tcatttcctg tctcactttg ccttaatacc
     2401 ctacgttaaa tgttaccaat ttgttgcttt ttatcacaat aagaaaacaa tatggcgcat
     2461 ttgtgcgcat ttttcagaaa tgtagatatt tttagattat ggctacgaaa tgagcatcgc
     2521 catgtcaccc tacatctcat aagaggatcg cttctgatga atgcactgac cgccgtacaa
     2581 aataacgctg tcgattcaga ccaggactat agcggattca ctctcatccc gtcggcgcaa
     2641 tccccgcgtc tgctggaact caccttcacc gaacagacga ccaaacagtt tctcgaacag
     2701 gttgccgaat ggcccgtgca ggcgctggag tacaaatcgt ttctgcgttt tcgggtaggt
     2761 aaaattcttg acgatctgtg tgcgaaccag ctgcaaccat tgctgttgaa gaccctgcta
     2821 aaccgtgctg aaggtgcgct gttgatcaat gcggtgggtg tcgatgatgt gaagcaagcg
     2881 gatgagatgg tgaagctggc gacggcggtg gcgcatctga ttggccgttc caatttcgat
     2941 gcgatgagcg gtcagtatta cgcgcgtttt gtagtgaaaa atgtcgataa ctcagacagc
     3001 tatctgcgtc agccgcaccg cgtaatggag ctgcacaacg acggcaccta cgtcgaagag
     3061 atcactgact atgtgctgat gatgaaaatc gacgagcaga acatgcaggg tggaaattcg
     3121 ttgctgctgc atctcgatga ctgggaacat ctggaccact atttccgcca tccaatggcg
     3181 cgtcgcccga tgcgctttgc cgcgccgccg agcaaaaacg tcagcaaaga tgttttccat
     3241 ccagtgttcg acgttgatca acagggtcgc ccggtgatgc gctatatcga ccagttcgtc
     3301 cagccaaaag acttcgaaga aggcgtgtgg ttgagcgagc tttcggacgc cattgaaatc
     3361 agtaaaggca ttctttctgt acccgttccc gttggcaaat tcctgttgat taacaacctg
     3421 ttctggctgc acggtcgcga ccgctttact ccgcacccgg atctgcgccg tgaactgatg
     3481 cgtcagcgtg gctatttcgc ttacgccact caccactatc agacgcatca gtaagcgcaa
     3541 aggaattgag cggatgtatg attttgtgat tattggcggc ggcatcatcg gcatgtcgac
     3601 cgccatgcaa ctgattgatg tctatccgga tgcccgcatt gcgttgctgg aaaaagagtc
     3661 cggcccggcc tgtcatcaga cgggccacaa cagcggcgtg atccatgccg gggtttatta
     3721 cacgcccggt agcctgaagg cgcagttttg cctggcggga aaccgcgcca ctaaagcctt
     3781 ttgcgatcaa aacggcattc gctatgacaa ctgcggcaag atgctggtcg ccacctctga
     3841 actcgaaatg gaacggatgc gcgcgttgtg ggaacgcacg gcggcaaacg gtatcgagcg
     3901 cgagtggtta aacgccgatg aactgcgcga gcgcgaaccg aatatcaccg ggcttggcgg
     3961 tatttttgtg ccgtccagcg gcattgtcag ctaccgcgaa gtcacggcgg cgatggcaaa
     4021 aattttccag gccagaggcg gcgagattat ctataacgcc gaagtcagcg gccttagtga
     4081 gcataaaagc ggcgtggtga tacgtacccg tcagggcagc gactatgaag catcgacgct
     4141 gattagctgt tccgggctga tggctgaccg gctggtgaaa atgctcggcc tcgaaccggg
     4201 ctttattatc tgcccgttcc gcggcgagta tttccgcctg gcgccggagc ataaccagat
     4261 tgttaaccat ctgatttacc ccattcccga cccggcaatg ccgtttttgg gcgtgcatct
     4321 cacccgcatg atcgacggca gcgtcaccgt cgggccaaac gcggtgctgg ctttcaaacg
     4381 cgaaggctat cgcaagcgcg acttctcatt tagcgacacg ctggagattt tgggctcgtc
     4441 ggggattcgc cgggtgctgc aaaaccatct acgctcagga ctgggcgaga tgaaaaactc
     4501 gctgtgcaaa agcggctatc tgcggctggt gcaaaagtat tgtccccggc tttcgttaag
     4561 cgatctccag ccctggcccg ccggtgtgcg ggcgcaggcg gtatcgccgg acggcaagct
     4621 gattgacgat tttctgtttg tcaccacccc gcgcacgatc cacacctgca atgcgccctc
     4681 cccggcagcg acatcagcaa ttcctattgg tgcgcatatt gtcagcaagg tacaaacgct
     4741 gttggcaagc cagagtaacc ccggacgcac gctgcgagcg gcacgtagtg tggatgcctt
     4801 acacgccgcg tttaatcaat aacctttgaa aacaggatgt agcgatgaaa ctgaacgaca
     4861 gtaacttatt ccgccagcag gcgttgatta acggggaatg gctggacgcc aacaatggtg
     4921 aagtcatcga cgtcaccaat ccggcgaacg gcgacaagct gggtagcgtg ccgaaaatgg
     4981 gcgctgatga aactcgcgcc gctatcgacg ccgccaaccg cgccctgccc gtctggcgtg
     5041 cgctcaccgc caaagaacgc gccaacattc tgcgcaactg gttcaatttg atgatggagc
     5101 atcaggacga tttagcgcgc ctgatgaccc tcgaacaggg taaaccgctg gctgaagcga
     5161 aaggcgaaat cagctacgcc gcctctttta ttgagtggtt tgccgaagaa ggcaaacgca
     5221 tttatggcga caccattccc ggtcatcagg ccgataagcg cctgattgtg atcaagcagc
     5281 cgattggcgt tacagcggcc atcacgccgt ggaactttcc ggcggcaatg attacccgca
     5341 aagccggtcc ggcgctggcg gcaggctgca ccatggtgct gaagcccgcc agtcagacgc
     5401 cgttctctgc gctggcgctg gcggagctgg cgatccgcgc gggcattccg gctggcgtgt
     5461 ttaacgtggt caccggttcg gcgggcgcgg tgggtaatga actgaccagc aacccgctgg
     5521 tacgcaaact gtcgtttacc ggctcgaccg aaattggccg ccagttaatg gaacagtgcg
     5581 cgaaagacat caaaaaagtg tcgctggagc tgggcggcaa cgcgccgttt atcgtctttg
     5641 acgatgccga tctcgataaa gccgtggaag gcgcactggc ctcgaaattc cgcaacgccg
     5701 ggcaaacctg cgtctgcgcc aaccgtttat acgtgcagga cggcgtgtat gaccgctttg
     5761 ccgaaaaatt gcagcaggcg gtgagcaaac tgcatatcgg cgacgggctg gataaaggcg
     5821 tcaccatcgg gccgctgatc gatgaaaaag cggtagcaaa agtggaagag catattgccg
     5881 atgcgctgga aaaaggcgcg cgcgtagttt gcggcggtaa agcacatgaa cgcggcggca
     5941 acttcttcca gcccaccatt ctggtggacg tgccggccaa cgccaaagtg tcgaaagaag
     6001 agaccttcgg ccccctcgcc ccgctgttcc gttttaaaga tgaagccgat gtgatcgcgc
     6061 aagccaatga caccgagttt ggccttgccg cctatttcta tgcccgtgat ttaagtcgcg
     6121 tcttccgcgt gggcgaggcg ctggagtacg gcatcgtcgg catcaatacc gggattattt
     6181 ccaatgaagt ggccccgttc ggcggcatta aagcctcggg tctgggtcgt gaaggttcga
     6241 agtatggcat cgaagattac ttagaaatca aatatatgtg catcggtctt taactggaga
     6301 acgcgaatga gcagcaataa agagttaatg cagcgccgca gtcaggcaat tcctcgtggc
     6361 gttgggcaaa ttcaccccat tttcgctgat cgcgcggaaa actgccgggt gtgggacgtt
     6421 gaaggccgtg agtatcttga tttcgcgggc ggcattgcgg tgctcaatac cgggcacctg
     6481 catccgaaag tggttgccgc ggtggaagcg cagttgaaaa aactgtcgca cacctgcttc
     6541 caggtgctgg cttacgagcc gtatctggag ctgtgcgaga ttatgaatca gaaggtgccg
     6601 ggcgattttg ccaagaaaac gctgctggtt acgaccggtt ccgaagcggt ggaaaacgcg
     6661 gtgaaaatcg cccgcgctgc caccaaacgt agcggcacca tcgcttttag cggcgcgtat
     6721 cacgggcgca cgcattacac gctggcgctg accggcaagg tgaatccgta ctctgcgggc
     6781 atgggcctga tgccagggca cgtttatcgc gcgctttatc cttgcccact gcacggcatc
     6841 agtgaagatg atgctatcgc cagtatccac cgaattttta aaaatgatgc tgcgccggaa
     6901 gatatcgccg ccatcgtgat tgagccggtt cagggcgaag gcggtttcta cgccgcgacg
     6961 cctgcgttta tgcagcgttt acgcgcgctg tgtgacgagc acgggatcat gctgattgcc
     7021 gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt ttgcgatgga gcaaatgggc
     7081 gtggcaccag atctcaccac ctttgcgaaa tcgatcgcag gcggcttccc actggcgggc
     7141 gtcaccgggc gcgccgaagt gatggatgcc gtcgctccag gcgggctggg tggcacctat
     7201 gccggtaatc cgattgcctg cgtggcggcg ctggaagtgt tgaaggtgtt cgagcaggaa
     7261 aatctgctgc agaaagccaa cgatctgggg cagaagttga aagatggatt gttggcgatc
     7321 gccgaaaaac accctgagat cggcgacgta cgcgggctgg gggcgatgat cgccatcgag
     7381 ctgtttgaag acggcgatca caacaagccg gacgccaaac tcaccgccga gatcgtggct
     7441 cgcgcccgcg ataaaggcct gattcttctc tcctgcggcc cgtattacaa cgtgctgcgc
     7501 atccttgtac cgctcaccat tgaagacgct cagatccgtc agggtctgga gatcatcagc
     7561 cagtgttttg ctgaggcaaa gcagtagcgc cgctcctatg ccggaggcga cgctgcgcgt
     7621 cttgtccggc ctacggggat ccaggtcgga taaggcgttt acgccgcatc cgacaatctg
     7681 tacgtgaaca ggaataaatc tatgttggcc gggtaaggcg gagccgctct ccggcaaaaa
     7741 gaatcaataa caattatacg cgtgacccga cgcgggaaat gtcggggcgc tctcccaagt
     7801 gacacacttt cgagaggatt caggatgggg caatcatcgc aaccacatga gttaggcggc
     7861 gggctgaagt cacgccatgt caccatgttg tctattgccg gtgttatcgg cgcaagtctg
     7921 tttgtcggtt ccagcgtcgc catcgccgaa gcgggcccgg cggtattact ggcctatctg
     7981 ttcgccgggc tactggtggt tatgattatg cggatgttgg cggaaatggc ggttgccacg
     8041 cccgataccg gttcgttttc cacctatgcc gataaagcta ttggtcgttg ggcgggttat
     8101 accatcggct ggttgtactg gtggttttgg gtactggtta tcccgctgga agccaacatc
     8161 gccgccatga tcctgcactc atgggttcca ggcattccca tctggttatt ttccctcgtc
     8221 attacccttg ccttaactgg cagtaattta ttaagcgtta aaaactacgg cgaatttgag
     8281 ttctggctgg cgctgtgcaa agtcatcgct atcctggcct ttattttcct tggtgcagtc
     8341 gcaattagcg gtttttaccc gtatgccgac gtgagcggga tctcaagatt gtgggatagc
     8401 ggcggcttta tgcccaacgg cttcggtgcg gtattaagcg cgatgttgat caccatgttc
     8461 tcgtttatgg gcgcagaaat tgtcaccatt gccgccgcgg aatccgacac gccggaaaaa
     8521 catattgtcc gcgctaccaa ctcggttatc tggcgtattt ctatcttcta tttgtgctct
     8581 atttttgtcg tagtggcgtt aataccgtgg aatatgccgg ggctgaaagc cgttggatct
     8641 tatcgctcgg ttctggaatt gctcaatata ccccatgcga aattaatcat ggactgcgtg
     8701 atattacttt ccgtaaccag ttgcctgaac tcggcgctgt ataccgcgtc aaggatgctc
     8761 tactccttaa gccgtcgcgg tgatgcgccc gcggtaatgg gcaaaatcaa ccgcagtaaa
     8821 accccgtatg tggcggtgtt actctccacc ggagcggcat ttttaacggt cgtggtgaac
     8881 tattacgcac ctgcgaaagt gtttaaattc ctgatcgaca gctccggtgc tatcgccctg
     8941 ctggtttatt tagtcatcgc cgtttcacag ttgcggatgc gtaaaattct gcgagcagaa
     9001 ggaagcgaaa ttcgcttgcg catgtggctt tacccgtggc tcacctggct ggtaatcggc
     9061 tttattacct ttgtgttggt ggtgatgtta ttccgcccgg cgcaacagtt agaagtgatc
     9121 tctaccggct tattagcgat agggattatc tgtaccgtgc cgattatggc gcgctggaaa
     9181 aagctggtat tgtggcaaaa aacacccgtt cataatacgc gctgataatg atcaggagtc
     9241 acaccatgac cattacgtct ctggatggct atcgctggct gaagaacgat attattcgcg
     9301 gtaattttca accggatgaa aaattacgaa tgagtttgct gacatcgcgt tatgcacttg
     9361 gcgttgggcc gttacgggaa gctctttcgc aactggtggc ggaacggctg gtcacggtgg
     9421 tgaatcaaaa agggtatcgg gtggcgtcta tgtcagagca ggagctgctc gatattttcg
     9481 acgcccgcgc caatatggaa gcgatgttag tgagtctggc gattgcccgc ggtggcgatg
     9541 agtgggaggc agacgttctc gcaaaagcgc atctgctgag taagcttgag gcctgtgacg
     9601 ccagcgagaa aatgcttgat gagtgggatc tgcgtcatca ggcgtttcat actgcaattg
     9661 tggcgggctg tggttctcac tatttgctgc aaatgcgtga acggttgttt gatctggcgg
     9721 cgcgttatcg atttatctgg ctgcggcgaa cggtgctttc ggtggaaatg ctggaggata
     9781 aacacgatca gcaccagacc ctgactgcgg cggtactggc gcgagatacc gcgcgcgcca
     9841 gtgagttaat gcgccagcat ttactgacgc caattcccat tatccagcag gcgatggctg
     9901 gcaattaact actcttccgg aatacgcaac acttgccccg gataaatttt atccgggctt
     9961 tttagcatcg gtttattcgc ttcgaagatt ttattgtaca gattagcgtt accgtagacc
    10021 tgtttggaaa tggcactcag agtgtcgcca gacttaacgg tataaaactg gctggcagtg
    10081 gctggtgtcg ccgttttcac ctgatcatca acactggcaa taccggaaat attccccacc
    10141 gcaacaagga ttttctcctt cgcctcctga ctcaggccgt caccagtgac cgtcgctttg
    10201 ccatcggcaa tttgaatgtt cactttatcg gcatcaggta taccggtttt gctcagatgc
    10261 tcctgcacct tcttcgcctg atcatcttta tcgtgctgac ctgtaaccgc gtcccagagt
    10321 ttttctccgg catctttcac aaaattgaac agacccatag ctacctcatt cgttaacgga
    10381 aacactcaga aagtgtagta gaggaacgcg gcgcgttaac gccgcgggga tgagcacagg
    10441 tttaatcgcg ggtttgcacc cagaacgcgt gaatcaaacc ggggatatac ccaagcagtg
    10501 tcaacagaat attaataatg aacgcccaac cgaacccttt accgagcagc acgccgagcg
    10561 gcggcagaat gatggtgatg acgattctcc agaaacccat atgtactccc tataagaaaa
    10621 ttacttattg tttaaaaaga gattttatct cttaagcgta gtaattttag cggaggctgc
    10681 cagtttcagc ttacgctttt actctcattt acgctgctga ggctggtata tgttgcattt
    10741 ttagtttaga gtttacttaa tttagaaaat acttaaataa ctatgaccga actcacgcaa
    10801 ttacaggcca gtgctgaaca ggcagcggcc ttattgaaag caatgagcca ccctaaacgg
    10861 ttgctgattc tgtgcatgct tagcggttcc cccggcacca gcgcgggaga gctgacgcgc
    10921 attaccggac tgagcgcctc tgcgacatca cagcatctcg ctcgtatgcg ggacgaaggg
    10981 cttatcaaca gccaacggga tgcccaacgc attctatatt ccattaaaaa tgaggcggta
    11041 aatgccatta tcgccaccct gaaaaatgtc tattgtccgt aaggagtcac tatggctttg
    11101 acaaccattt cgccgcatga tgcacaagaa ttaatcgcac gcggcgcaaa gttaatcgat
    11161 attcgtgacg ctgatgaata tcttcgtgaa catattcctg aagcagatct ggctccatta
    11221 tccgtgctgg aacagtcagg tctcccggct aaattacgtc gcgatcaaat tatattccac
    11281 tgccaggcag gtaaacgcac cagtaataac gccgataaat tagcagcgat tgccgccccc
    11341 gcagaaatct ttttactcga agatgggatt gatggctgga aaaaagcagg attaccagta
    11401 gcggtaaata aatctcaacc cttgccgtta atgcgccagg tgcagatcgc tgcgggtggt
    11461 ttaatattaa tcggcgttat actgggttat accgtaaata gcggtttctt cttattaagc
    11521 ggctttgttg gtgccgggtt actgtttgca ggaattagcg gtttttgtgg gatggcaagg
    11581 ttgttagata agatgccgtg gaaccaatga gcttgagaag cgacgccgga cgcgccctag
    11641 cagcgacatc cggcctcagt aattagatca ggaaatcgtc gagagattta ccttctgcca
    11701 gcgcctgagc gattggcttc ggtgtacggc cctgaccggt ccaggtttta gtttcaccgt
    11761 taacatcggt gaatttatat ttcgccggac gcggctggcg ttttttacca gcacgtggtg
    11821 cagcagcaga gctattaccc aataactctt ccgggttaat tccgtcagct ttcatcagct
    11881 ccagccaggt gctaattttt tcctggcgct ctgccagttc acgctgctgc tgttcttctt
    11941 cttcacgtct ttctttagtg acaaccctga atttttcgag catttcttca agaacgtcaa
    12001 tggagaattc gcgagccatc gcacggaggg tgcgaatgtt atttaaactt tgtaacatta
    12061 cggacataat aaagaaaacc ttttaacgcc aaaacaaaaa agtatttcgt cgcgtagtgt
    12121 aagcgatcac ttattatatt tccacaaaat ttaatctatt tacagtcctg cgcgagatta
    12181 tttcagctgt tattttctga aactctcaga ataatttcaa gattatatat tagccatttt
    12241 gatgagtaat ttagattatt ggtgaacaga acggcgctct ctgctcgcta attccgcaag
    12301 cctgacaaaa ccaaagtgaa taaaaatgca atataacaga ataaaataaa acacccctca
    12361 ctggtgcttt ctgctgttaa tgtacatttt ctggcataaa aaatgctgta aatgacccgg
    12421 tcacgacata caccgaattc tctacgctta tagcgttacc tcacccccaa actttcatta
    12481 gtgaaattaa aggaaaggtc atatccaatt gatttaattg aaaataaatc atatcggcat
    12541 gtaaaaatcc acaatgcaca aaaaacgagc cgttacggaa tattttatct acaaaaactg
    12601 actaaataaa aatttttcac taattgatta gtcatagcca gcgatatacg ctatgcgaaa
    12661 atgcagatgg caatgagatc cactgctttc atctccatta acatcccatt acgcttttat
    12721 taaggagcat tagcatgttc tcaccgcagt cacgcttgcg tcatgcagtt gcagatacgt
    12781 tcgcgatggt tgtttactgt tctgtcgtga acatgtgtat tgaagttttc ctctccggaa
    12841 tgagcttcga acagtctttt tattccagat tggtagcgat tccggtgaac atcttaattg
    12901 catggccata cggtatgtac cgcgatctgt ttatgcgcgc ggcacgcaaa gttagcccgt
    12961 cgggctggat aaaaaatctg gcggatatcc tggcttatgt gacgttccag tcaccggtgt
    13021 atgtggcgat cttgttagtg gtgggcgcag actggcatca gattatggcg gcggtcagtt
    13081 caaacatcgt tgtttcgatg ttgatggggg cggtttatgg ctacttcctc gattattgcc
    13141 gccgactgtt taaagtcagc cgttaccagc aggtaaaagc ctgactcttc ttttcgcgac
    13201 tggcatcgcc agtcgcgtct ttaactggct tcgccaaata aaccattcaa ataacgttca
    13261 agcgcaacac gcgaactaaa gccatgcggg ataccgtaat gctcgtgtcg ctcgcctgct
    13321 aaatagagcg gaaattgctg gtaatgatcg caagttttaa tatcggaagc gggttcagcc
    13381 agtgtcgaac tgcgttcttt taatgttgga tgaattacca gagcggtacg tcccattcgc
    13441 gcttcgcgat taacatagac ataattttcc ccacggcggt aaccatacgc tttctgggtt
    13501 accacatcga cagtaaaacc gactttttca agtacgcgcg ccacctcgtc tggtcgtaaa
    13561 tacatatttg atcctcgtta ttatcaaccg cgggcctacc ttacctgatt gcgcattcac
    13621 aacgctttca gaaaagtcca taaagccgcg atgaacctca gttaagaaaa ttatggtcta
    13681 cactgaaaat tacatcgaat tctaatgggg gatcatatgt ttaaccgacc gaaccgcaac
    13741 gacgttgatg atggcgtgca ggatatccag aatgatgtca atcaattagc tgacagcctc
    13801 gaatctgtat tgaagtcctg gggcagcgac gccaaagggg aagctgaagc cgcacgcagt
    13861 aaagctcagg cattgctgaa agaaaccagg gcgcgaatgc atggtcgtac tcgcgtccag
    13921 caagccgcgc gcgatgccgt aggctgcgcg gattcttttg ttcgtgaaag accctggtgt
    13981 agtgtgggaa ctgctgctgc ggtaggtatt tttatcggtg cgttgttgag catgcgtaaa
    14041 tcgtagtgag acgatgacaa gcagtagtct gacttctgaa aagtactcag tgcgtcaacg
    14101 ccttgtccgc ctacaggcct gaaaaacata gcgcatcagg ggattttgcc ctggtcacca
    14161 gtcttatagg accccgaagc ctggcttcgg ggttattaat ttaaagcact gcgcaattgc
    14221 tgtgctacct gtcgcgccat actttcgcta acgatattcg taaacgagag caataatcct
    14281 ccgtcctcgc ttgagcggat acgccagtcg ctaagtgcct gtacggcaag accagcagca
    14341 ttggctttat gcacgaatgt cacgtcatta cctgctagtt tgatcaccat ctggataccg
    14401 cctttttgcg gcacaacctg aaatccctgc tgcgtaagtg cctgctcaat ccactgccgt
    14461 cgctgggcat aatgctggcg cattttcttc agatgccgcc agaaatgccc ctcacggatg
    14521 aaatctgcca gtgtgttctg ccacagtacg ggtacagcac atggttccag tgacgcctgc
    14581 tgccggaatt gcgaaatctg ctccaccggc accaccagcc acgcacagcg caatgcagga
    14641 aatagcgctt tgctgaatgt tccggcataa atcacccgct gcggcgcgtc gagacttttt
    14701 agtgccggta acggcttacc gtgatagcga aactcactgt cgtaatcatc ttcaataatc
    14761 catgcctgac tacgctctgc ccattccagt atctgatacc tacgcgctaa agagagtgcc
    14821 acacccagcg ggctttggtg tgccggagta atcagggcaa aacgcgcatc aggataattt
    14881 tgtattccgc ttgtgacatc cagtccgttg tcatcaaccg gcacaggtaa aatttccaca
    14941 ccgtggcgag tgacaatcgg gcgaatcagc ggaaagccgg gatcttccat ccacatcccg
    15001 tctcccggtt gcgccagagc gtgcagaatt aaagccattg aggccgcata accgtgagtg
    15061 ataaagacct gctctggctg acaatcaatc ccacgtgaaa cgcgtaaata atcaacaata
    15121 gcctcgcgca aggccgcctc gccgcagaca tcgcctaatg ccaaatcaaa gcgcgtctgg
    15181 gtacgcagac ggcggcccat cacccgcgcc cacaattcac gcggaaaaag atcaagtgca
    15241 ggtagcccca tctgaaaagg cagcggctgc tgactttcac cagcaaaagc cacgacatcc
    15301 acagcagact gttgcggatg tatctgctca ctgacaaagg tacccgcctg tccccgcctg
    15361 accagccatc cctgcgccac cagctcagca taagcatttt cgacagtgga tcgagagaca
    15421 cccagctcct gcgaccaggt acggctcgaa ggtagccttg cgccaggttt cagttttcct
    15481 tgctcgataa ccgtttttaa ctgacgggcg atatcctgat aacgtggcat atggtctgct
    15541 tttttaggta atttatggct ctctttagca gtccattata gtgctacatt gcgggcgtta
    15601 ataagaggag aaactgtatg actacattac gtcagcctta ctacgaactt agcccggcag
    15661 tgtataacgc actggtgcag gccaaaacgg cactggaaaa tagcacgctg gataccacgt
    15721 tgatggagtt aatttatttg cgcgtctcgc aaatcaacgg ctgcgcattt tgtctggaga
    15781 tgcacagcaa agcattgcgc aaatccggtg tgccacaaca caaactggac gccctggcag
    15841 gctggcgcgt aagccatcat tttgatgaac gcgagcgagc ggcgctggca tgggcggaat
    15901 ctgtgaccga tattgccaga acccatgcgg aagacgaggt ttatcagcct ttgcttgagc
    15961 atttcagcgc agcagaaatc agcgacttaa cgtttgccat cgggctgatg aattgtttta
    16021 accgtctggc cgtttccatg cggatgtaat ttgctgttgc cgggtatgcg cccggcaaaa
    16081 aaacacaaaa tcccctaccc cgttgagcca ttcaccggtg gcgtaatcaa tctaaaatta
    16141 ccatatcttg tgtggttgaa tcttttttca actacatcta gtatctctgt atcaacagag
    16201 agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat acgaatcatg
    16261 cgcattacta tttacactcg taacgattgc gttcagtgcc acgccaccaa acgggcgatg
    16321 gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga agcggcagaa
    16381 gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga tcttagctgg
    16441 tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc ggccagtgca
    16501 tgagccagct cgtctacttc tccagcagct ccgaaaacac gcagcgtttt atcgaacgtt
    16561 taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag gtagacgagc
    16621 cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg gtgccacgac
    16681 aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc gttattgctt
    16741 cgggtaatcg caactttggt gaggcgtatg gccgcgccgg agatgtgatt gcccggaaat
    16801 gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac gcaaagcgat atcgaaaacg
    16861 ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga cgcagaaaac
    16921 gatggattac cacgcgctga atgcgatgct taacctctac gatagcgcag gtcgcattca
    16981 gttcgataaa gaccgccagg ccgttgacgc ctttatggcg acgcatgtgc gtccgaacag
    17041 tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag gttactatga
    17101 tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg cccacgcaca
    17161 caccagcggt tttcgtttcc agacattcct cggggcatgg aagttttaca ccagctatac
    17221 gttgaagaca ttcgacggta aacgttatct ggaagatttt gccgatcgag tcacgatggt
    17281 ggcgctgacg ctggcacaag gcgatgagac gctggcgttg caactgacag atgaaatgct
    17341 gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc agcagcgcgg
    17401 cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga ttggtcgggc
    17461 ggtaaattcc gcactgcaac tgtcgaaacg cggcggcggc gtagcatttt tgctgtcgaa
    17521 tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg gcgtaattcc
    17581 ggtgatgaaa atgctggaag acgcattttc ctatgccaac caactcggcg ctcgtcaggg
    17641 ggctggtgca gtctatttac atgctcatca tcccgatatt ctgcgttttc tcgacacgaa
    17701 acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgcttggcg tggtgatccc
    17761 ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt cgccttatga
    17821 cgtagagcga gtttatggca agccgtttgc cgatgtggcc atcagccaac actatgacga
    17881 actggttgcc gatgaacgca ttcgcaaaaa atacctcaac gcccgtgatt tcttccagcg
    17941 actggcagaa atccagtttg agtccggcta tccctacatc atgtatgaag acacggtaaa
    18001 ccgtgctaac cctgtcgccg ggcgcataaa tatgagtaat ctctgctcag aaattttgca
    18061 ggttaacagc gcctcagagt atgacgagaa tctcgactat gcccgcacag gccatgatat
    18121 ctcctgcaat ttaggttcgt tgaatattgc gcacaccatg gattcccccg attttgcccg
    18181 cacggtagag actgccgtgc gcggtttaac ggcagtatca gatatgagtc atatccgcag
    18241 cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg ggcagatgaa
    18301 tttacacggc tatctggcgc gagaaggcat cgcttatggt tcgccggaag cactggattt
    18361 caccaatctc tatttctatg ccatcacctg gcacgcactg cgtacctcga tgttgctggc
    18421 acgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca gtggtgaata
    18481 ttttagccaa tatctgcaag ggaactggca gccgaaaacg gggaaagttg gcgaactgtt
    18541 tgcccgtagc ggtattacgt tacctacccg tgagatgtgg gcgcaactgc gcgacgacgt
    18601 gatgcgctac ggcatttaca accagaatct tcaggcggtg ccgccaacag gttctatctc
    18661 ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag agatacgcaa
    18721 agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg agaatctggc
    18781 gctgtatcag gacgcttacg aaattggcgc agaaaagatc atcgacacct acgcggaagc
    18841 gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata ccgccaccac
    18901 tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa cgctctatta
    18961 catccggctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg tctcctgtgc
    19021 actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga acaagatatc
    19081 tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc taccagaaaa
    19141 ggtgccgctg tcgaacgata ttcctgcctg gcagacatta actgtcgtag aacaacaact
    19201 gacgatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg ttatcggcgc
    19261 gccttcgctg atgcccgatg cactcacgcc tcatgaagaa gcggtattat cgaatatcag
    19321 ctttatggaa gcggttcatg cccgctctta cagttcgatt ttctcgacgc tgtgccagac
    19381 caaagatgtc gatgccgcct acgcctggag tgaagaaaac gcgccgttgc agcgaaaagc
    19441 acagattatt cagcaacatt accgcggtga tgatccgctg aaaaagaaaa tcgccagtgt
    19501 gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt tttccagccg
    19561 cggaaagctg accaataccg cggacctgat ccgtctgatt atccgcgatg aagcagtcca
    19621 cggttactac ataggctata aatatcagaa aaacatggaa aagatatctc tgggacaacg
    19681 tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctacgaca acgagttgca
    19741 atacaccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag cgtttctctg
    19801 ttacaacgcc aataaggctt tgatgaatct gggctacgaa ccgttatttc ccgcagaaat
    19861 ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg aaaatcacga
    19921 tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa cagaatatga
    19981 agactggaat ttctgagggt gttattttca aaaatatcac tacccgcgac agggaaataa
    20041 ttcccgccaa atagcttttt atcacgcaaa tattttgtgg tgatctacac tgatactctg
    20101 ttgcattatt cgcctgaaac cacaatattc aggcgttttt tcgctatctt tgacaaaaaa
    20161 tatcaacttt tctcgatttg ctctcagccc ttatatcacg ggaaattccg gcgatttgct
    20221 cgcatcaata ttcatgccac atttgccatc aggggttgcc tcagattctc agtatgttag
    20281 ggtagaaaaa agtgactatt tccattgggt aatatatcga cataaacaaa taacaggaat
    20341 ctttctattg catggcaatt aaattagaaa ttaaaaatct ttataaaata tttggtgagc
    20401 atccacagcg agcgttcaaa tatatcgaac aaggactttc aaaagaacaa attctggaaa
    20461 aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa ggcgagatat
    20521 ttgtcatcat gggattatcc ggctcgggta aatccacaat ggtacgcctt ctcaatcgcc
    20581 tgattgaacc cacccgcggg caagtactga ttgatggtgt ggatattgcc aaaatatccg
    20641 acgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag tcctttgcct
    20701 taatgccgca tatgaccgtg ctggacaata ctgcgtttgg tatggaattg gccggaatta
    20761 atgccgaaga acgccgggaa aaagcccttg atgcactgcg tcaggtcggg ctggaaaatt
    20821 atgcccacag ctacccgaat gaactctctg gcgggatgcg tcaacgtgtg ggattagccc
    20881 gcgcgttagc gattaatccg gatattttat taatggatga agccttctcg gcgctcgatc
    20941 cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa catcagcgca
    21001 ccattgtctt tatttcccac gatctcgatg aagccatgcg tattggcgac cgaattgcca
    21061 ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga tgaaattatc aataatccgg
    21121 cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat tagtcaggta ttcagtgcga
    21181 aagatattgc ccgccggaca ccgaatggct taattcgtaa aacccctggc ttcggcccac
    21241 gttcggcact gaaattattg caggatgaag atcgtgaata tggctacgtt atcgaacgcg
    21301 gtaataagtt tgtcggcgca gtctccatcg attcgcttaa aaccgcgtta acgcagcagc
    21361 aaggtcttga tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa acgcctctta
    21421 gcgagttgct ctctcatgtc ggacaggctc cctgtgcggt gcccgtggtc gacgaggacc
    21481 aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat cgtgaggggg
    21541 taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga cagtgccgcg
    21601 caatccgcag acgcctgggg tacaccggcg actgcaccga ctgacggcgg tggtgctgac
    21661 tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga tccgttccat
    21721 aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt cgttacccat
    21781 ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa cggtttccag
    21841 caattgctgc tgggtatgcc cgcaccggtg gcgattatcg ttttcgctct catcgcctgg
    21901 cagatttccg gggtcggaat gggcgtggcg acgctggttt cgctgattgc catcggcgca
    21961 atcggtgcct ggtcgcaggc aatggttact ctggcgctgg tgttaaccgc cctgctgttc
    22021 tgtatcgtca tcggtttgcc gttggggatc tggctggcga gaagtccgcg agcggcgaaa
    22081 attattcgtc cactgcttga tgccatgcag accacgccag cgtttgttta tctggtgcca
    22141 atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat catctttgct
    22201 ctgccgccga ttatccgtct gaccattctg gggattaacc aggttccggc ggatctgatt
    22261 gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt tcagttaccg
    22321 ctggcgatgc cgaccattat ggcgggcgtt aaccagacgc tgatgctggc cctttctatg
    22381 gtggtcatcg cctcgatgat tgccgtcggc gggttgggtc agatggtact tcgtggtatc
    22441 ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat cctcgccatt
    22501 atcctcgatc gtctgacgca ggccgttggg cgcgactcac gcagtcgcgg caaccgtcgc
    22561 tggtacacca ctggccctgt cggtctgctg acccgcccat tcattaagta actctgcact
    22621 tgcccggtga cgccgggcat atcaccctgc caaaaaaagg aataacaatg cgacatagcg
    22681 tactttttgc gacagcgttt gccacgctta tctctacaca aacttttgct gccgacctgc
    22741 cgggcaaagg cattactgtt aatccggttc agagcaccat caccgaagaa actttccaga
    22801 cgctgctggt cagccgcgcg ctggagaaat taggttatac cgtcaataaa cccagcgaag
    22861 tggattacaa cgttggctac acctcgcttg cttccggcga tgcaaccttc accgccgtga
    22921 actggacgcc actgcatgac aacatgtacg aagctgccgg tggcgataag aaattttatc
    22981 gtgaaggggt atttgttaac ggcgcggcac agggttacct gatcgataag aaaaccgccg
    23041 accagtacaa aatcaccaac atcgcacaac tgaaagatcc gaagatcgcc aaactgttcg
    23101 ataccaacgg cgacggaaaa gcggatttaa ccggttgtaa ccctggctgg ggctgcgaag
    23161 gtgcgatcaa ccaccagctt gccgcgtatg aactgaccaa taccgtgacg cataatcagg
    23221 ggaactacgc ggcgatgatg gccgacacca tcagtcgtta caaagagggc aaaccggtgt
    23281 tttactacac ctggacgccg tactgggtga gtaatgagct gaagccaggc aaagatgtgg
    23341 tctggttgca ggtgccgttc tctgcactgc cgggcgataa aaatgccgat accaaactgc
    23401 cgaatggcgc gaattacggc ttcccggtca gcaccatgca tatcgttgcc aataaagcct
    23461 gggccgagaa aaacccggca gcagcgaaac tgtttgccat tatgcagttg ccagtggcag
    23521 atattaacgc ccagaacgcc attatgcatg acggcaaagc ctcagaaggc gatattcagg
    23581 gccacgttga tggctggatc aaagcccacc agcaacagtt cgatggctgg gtgaatgagg
    23641 cgttggcagc gcagaagtaa tttttattcg ggcggataag gcgtttacgc cgcatccgcc
    23701 gttctgtaca caatgcctga tgcgacgctg gcgcgtctta tcatgcctac aaaccacacc
    23761 gcacatttca acaatctatc tttcatccca tattcatcaa catccgctat tattgatttc
    23821 cagcttaatc atcacctgat gaacaaaaat aatgaccaaa cctaatcatg agcttagccc
    23881 ggcgctaatc gtgctgatgt ctatcgccac cggtctggcg gtcgccagca actattacgc
    23941 ccagccattg ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt cggcaggctt
    24001 tattgttacc gccgcgcagt tgggctatgc cgccggtctg ctgtttcttg ttcccctcgg
    24061 tgatatgttt gaacgccgcc gcctgattgt ctcgatgacc ttactggcag cgggcggtat
    24121 gttaatcact gccagcagtc agtcactggc gatgatgatc ctcggtacgg cattaaccgg
    24181 tttattctca gtcgtggcac aaattctggt tccgctggca gcgacgctgg cttcaccgga
    24241 taaacgcggc aaagtggttg gcaccattat gagcggtctg ctgttgggga tattgctggc
    24301 acgaaccgtt gccgggttac tggcaaacct cggcggctgg cgcaccgtct tttgggttgc
    24361 ctcggtgtta atggctctga tggcgctggc attatggcgt ggtctgccac aaatgaaatc
    24421 agaaacccac ctcaactacc cacagttgtt gggttccgtt ttcagcatgt ttatcagcaa
    24481 taagatcctg cgcacccgcg cgttgctggg ctgcctgacc tttgccaact tcagcattct
    24541 ctggacctca atggcctttt tgcttgccgc tccacctttt aactacagcg atggtgtaat
    24601 tggtctgttt gggcttgcgg gagctgccgg agcgttgggc gctcgtccgg cgggcggttt
    24661 tgccgacaag ggcaaatcgc accacaccac aactttcggt ctgcttctgc tgttactttc
    24721 atggctggca atctggtttg gtcacacttc cgtactggcg ttgattatcg gcattctggt
    24781 gctggacctc accgtacagg gcgtgcatat cactaaccag acggtaattt atcgaataca
    24841 tcctgatgcg cgtaatcgcc tgaccgcagg ttacatgacc agctacttta ttggcggtgc
    24901 cgccggttcg ctaatttcag cctcagcctg gcaacatggc ggttgggctg gcgtttgtct
    24961 ggctggcgcg acgattgccc tggttaactt actggtctgg tggcgaggtt ttcatcgtca
    25021 ggaagccgca aattaagcaa tcagcaaccg ttttcgtggc tttacactta taagggtgtt
    25081 aagaagccca tcagtctgat aaggttaaga tattcattca gtctatttat aatattaaca
    25141 atcgttaagc gtacactcta tggaaagccc tactccacag cctgctcctg gttcggcgac
    25201 cttcatggaa ggatgcaaag acagtttacc gattgttatt agttatatcc cggtggcctt
    25261 tgcgttcggt ctgaatgcga cccgtctggg attctctcct ctcgaaagcg tttttttctc
    25321 ctgcatcatt tatgcaggcg cgagccagtt cgtcattacc gcgatgctgg cagcagggag
    25381 tagtttgtgg gttgctgcac tgaccgtcat ggcaatggat gttcgccatg ttttgtatgg
    25441 cccgtcactg cgtagccgta ttattcagcg tctgcaaaaa tcgaaaaccg ccctatgggc
    25501 gtttggcctg acggatgagg tttttgccgc cgccaccgca aaactggtac gcaataatcg
    25561 ccgctggagc gagaactgga tgatcggcat tgccttcagt tcatggtcat cgtgggtatt
    25621 tggtacggta ataggggcat tctccggcag cggcttgctg caaggttatc ccgccgttga
    25681 agctgcatta ggttttatgc ttccggcact ctttatgagt ttcctgctcg cctcttttca
    25741 gcgcaaacaa tctctttgcg ttaccgcagc gttagttggt gcccttgcag gcgtaacgct
    25801 attttctatt cccgtcgcca ttctggcagg cattgtctgt ggctgcctca ctgcgttaat
    25861 ccaggcattc tggcaaggag cgcccgatga gctatgaggt tctgctgctt gggttactgg
    25921 ttggcgcggc gaattattgc ttccgctatt tgccgctgcg cctgcgtgtg ggtaatgccc
    25981 gcccaaccaa acgtggcgcg gtaggtattt tgctcgacac cattggcatc gcctcgatat
    26041 gcgctctgct ggttgtctct accgcaccag aagtgatgca cgatacacgc cgtttcgtgc
    26101 ccacgctggt cggcttcgcg gtactgggtg ccagtttcta taaaacacgc agcattatca
    26161 tcccaacact gcttagtgcg ctggcctatg ggctcgcctg gaaagtgatg gcgattatat
    26221 aacccacaag aatcattttt ctaaaacaat acatttactt tatttgtcac tgtcgttact
    26281 atatcggctg aaattaatga ggtcataccc aaatggatag ttcgtttacg cccattgaac
    26341 aaatgctaaa atttcgcgcc agccgccacg aagattttcc ttatcaggag atccttctga
    26401 ctcgtctttg catgcatatg caaagcaagc tgctggagaa ccgcaataaa atgctgaagg
    26461 ctcaggggat taacgagacg ttgtttatgg cgttgattac gctggagtct caggaaaacc
    26521 acagtattca gccttctgaa ttaagttgtg ctcttggatc atcccgtacc aacgcgacgc
    26581 gtattgccga tgaactggaa aaacgcggtt ggatcgaacg tcgtgaaagc gataacgatc
    26641 gccgctgcct gcatctgcaa ttaacggaaa aaggtcacga gtttttgcgc gaggttttac
    26701 caccgcagca taactgcctg catcaactct ggtccgcgct cagcacaaca gaaaaagacc
    26761 agctcgagca aatcacccgc aaattgctct cccgtctcga ccagatggaa caagacggtg
    26821 tggttctcga agcgatgagc taacgcgtca tctcgctcaa aaatccagat ttataaaaga
    26881 aaaaatgacc ggccagcatc gcaacatgct ggcctttttg gcaagcaggt cggctcagcc
    26941 gatgagttaa gaagatcgtg gagaacaata tgagcgcaaa tgcggagact caaaccccgc
    27001 agcaaccggt aaagaagagc ggcaaacgta agcgtctgct cctccttctc accttgctct
    27061 ttataattat tgccgtagcg atagggattt attggttttt ggtactgcgt cacttcgaag
    27121 aaaccgatga cgcatacgtg gcagggaatc aaattcaaat tatgtctcag gtgtctggca
    27181 gcgtgacgaa agtctgggcc gataacaccg attttgtaaa agaaggcgac gtgctggtca
    27241 ctctcgaccc gacagatgct cgccaggcgt ttgaaaaagc caaaactgca ctggcttcca
    27301 gcgttcgcca aacccaccag cagatgatta acagcaagca gttgcaggcg aatattgagg
    27361 tgcaaaaaat cgccctcgcg aaagcacaaa gcgactacaa ccgccgtgtg ccgctgggca
    27421 atgccaacct gattggtcgc gaagagctgc aacacgcccg cgacgccgtt accagtgccc
    27481 aggcgcaact ggacgtcgcg attcaacaat acaatgccaa tcaggcgatg attctgggga
    27541 ctaaactgga agatcagcct gccgtgcaac aggctgccac cgaagtacgt aacgcctggc
    27601 tggcgctgga gcgtactcgt attgtcagtc cgatgaccgg ttatgtctcc cgccgcgcgg
    27661 tacagcctgg ggcgcaaatt agcccaacga cgccgctgat ggcggtcgtt ccagccacca
    27721 atatgtgggt ggatgccaac tttaaagaga cgcagattgc caatatgcgt atcggtcagc
    27781 cggtcactat caccaccgat atttacggcg atgatgtgaa atacaccggt aaagtggttg
    27841 gtctggatat gggcacaggt agcgcgttct cactgcttcc ggcgcaaaat gcgaccggta
    27901 actggatcaa agtcgttcag cgtctgcctg tgcgtatcga actggaccag aaacagctgg
    27961 agcaataccc gctgcgtatc ggtttatcca cgctggtgag cgtcaatacc actaaccgtg
    28021 acggtcaggt actggcaaat aaagtacgtt ccactccggt agcggtaagc accgcgcgtg
    28081 aaatcagcct ggcacctgtc aataaactga tcgacgatat cgtaaaagct aacgctggct
    28141 aatccagagg tgcgtgtgat gcaacagcaa aaaccgctgg aaggcgcgca actggtcatt
    28201 atgacgattg cgctgtcact agcgacattc atgcaggtgc tggactccac cattgctaac
    28261 gtggcgatcc ccactatcgc cgggaatctt ggctcatcgc tcagccaggg aacgtgggta
    28321 atcacttctt tcggggtggc gaatgccatc tcgatcccgc ttaccggctg gctggcaaag
    28381 cgcgtcgggg aagtgaaact gttcctctgg tcaaccatcg cctttgctat tgcgtcgtgg
    28441 gcgtgtggtg tctccagcag cctgaatatg ctgatcttct tccgcgtgat tcaggggatt
    28501 gtcgccgggc cgttgatccc gctttcgcaa agtctattgc tgaataatta cccgccagcc
    28561 aaacgctcaa tcgcgctggc gttgtggtcg atgacggtga ttgtcgcgcc aatttgcggc
    28621 ccaatcctcg gcggatatat cagcgataat taccactggg gctggatatt cttcatcaac
    28681 gtgccgattg gcgtggcggt ggtgttgatg acactgcaaa ctctgcgcgg acgtgaaacc
    28741 cgcaccgaac ggcggcggat tgatgccgtg gggctggcac tgctggttat tggtatcggc
    28801 agcctgcaga ttatgctcga ccgcggtaaa gagctggact ggttttcatc acaggaaatt
    28861 atcatcctta ccgtggtggc ggtggtggct atctgcttcc tgatcgtctg ggagctgacc
    28921 gacgataacc cgatagtcga tctgtcgttg tttaagtcgc gcaacttcac catcggctgt
    28981 ttgtgtatca gcctcgcgta tatgctctac ttcggcgcga ttgttctgct gccgcagttg
    29041 ttgcaggagg tctacggtta cacggcgacc tgggcaggtt tggcctctgc gccggtaggg
    29101 attattccag tgatcctgtc gccgattatc ggccgcttcg cgcataaact ggatatgcgc
    29161 cgactggtaa ccttcagctt tattatgtat gccgtctgct tctactggcg tgcctatacc
    29221 tttgaaccag gtatggattt tggcgcgtcg gcctggccgc agtttatcca ggggtttgcg
    29281 gtggcctgct tctttatgcc gctgaccacc attacgctgt ctggtttgcc accggaacga
    29341 ctggcggcgg catcgagcct ctccaacttt acgcgtacgc tggcggggtc tatcggcacg
    29401 tcgataacca caaccatgtg gaccaaccgc gagtcgatgc accatgcgca gttgactgag
    29461 tcggtaaacc cgttcaaccc gaatgcccag gcgatgtaca gtcaactgga agggcttggg
    29521 atgacacaac agcaggcatc aggctggatt gcccagcaga tcaccaatca ggggctgatt
    29581 atttccgcca atgagatctt ctggatgtcg gccgggatat tcctcgtctt gctggggctg
    29641 gtgtggtttg ctaaaccgcc ctttggcgca ggtggcggcg gaggcggtgc gcactaagta
    29701 caactaagcc agttcatttg aactggcttt tttcaattaa ttgtgaagat agtttactga
    29761 ctaaatgtgc agttcctgca acttctcttt cggcagcgcc agttcttcgt tgctgttgat
    29821 gcgcacgtca cgttccagaa tgctacgcgc aatatcctgc gcttcctgca acgagtgcat
    29881 ctggtaagtg ccacactggt agacgttcag ctccggaatc tgattctgat cctgcacttt
    29941 cagcacgtct tccattgccg ctttccagac atcagcaaca cgctgctcat ctggcgtacc
    30001 aatcagactc atataaaaac cggtgcggca gcccattggc gagatatcga taatctctac
    30061 accattaccg ttaagatggt tacgcataaa accagcaaac aggtgctcca gggtatggat
    30121 ccctctttct ggcatcactt ctttgttcgg cacgcagaag cgcagatcga acacggtgat
    30181 tgcgtcgcca tgcggggtgt tcattgtttt cgccacccga actgcaggcg cttccatccg
    30241 ggtatgatcg actgtgaagc tatctaacaa cggcatttag ccacctccgg taattttttt
    30301 aaaaattttc tgaactcttt cttcccaggc gagtctgagt atatgaaaga cgcgcatttg
    30361 ttatcatcat ccctgaattc agagatgaaa ttttggccac tcatgagtgg cctttttctt
    30421 ttctgtcagg cgtgtttttc cagccacacc gcaaacggtt cggtatctgc ggcttccatt
    30481 tcctgctgac ggcgttcaga tgcctcgcgc tcggctacaa aatcctcttc gcgcagaatt
    30541 tccagcggct cttcacgcag cagattacgg tacgcttcag caaacgcttt gcctgttccg
    30601 ccaataccag tatcaatcat agaccttaag atacgggcag agaaagtcag atcgggatta
    30661 tcgaagcagg caaccagttc atcacacact ttctgatacg cttcgccgcc gttaatgcta
    30721 tccagcgttt gcgcgacgcg tttcagatcg cggaacagat ctttacccac ctgcggtaac
    30781 gggaactgtg cggtttcgca gccgataccc agcgtcagac ccggtttgcg accttcgagg
    30841 atcacccggt tccagttaac gcgtgtacag gcaagttcgc tactgctcat ttccggtgca
    30901 tcagccagcg cacaccagac cataaacagg tcgaggaatc gcacctgctg ttcatctaca
    30961 ccaatcggcg agaacgggtt gatgtccagc gaacgcactt caatatattc aatgccgcca
    31021 cgtaacagcg catcagaagg cgactcgccg ctgcgggtaa cgcgttttgg acgaatcggc
    31081 gcgtacagtt cgttttcaat ctgcagcacg ttgctgttga tttgcagcct cttaccgtct
    31141 ttatcaatac caatcttcgc gtactcttcc gatggcgttt tgattgcctg tttcaggccc
    31201 gctacgtact cgtaaagatc gttgaaggta ataccaagat tgctttgcga tttattggta
    31261 taaccgagat cgctcaaacg aagagaggtc gcatacggca ggtaatacat accgcactcg
    31321 gttttctcaa acggcagcga cgttggttta ccttgcagga atgaagagca tattgccgga
    31381 gacgcgccaa acagataagg aatgacccaa ccgaagcgat agtagttgcg aataacgcgg
    31441 aaatagcccg cagagatttt ctctttagca tcagcgcccg aaatatcgcc acacttcgct
    31501 tgccagaatg ccataggcaa agagaagttg taatgcacgc cggagatggt ttgcatcagc
    31561 gcgccgtagc gatttttcag cccttcacgg tacagcgttt taaagcgtcc ggtgttagaa
    31621 gtgccgtact gcgccagttc gatgtcctga ccttctgcga tgtagcatgg catacttaac
    31681 ggccacatcc gctcatcgcc catattgcgc gccgtataac gatgcagatc gcgcataaag
    31741 gtcagcatat gttcaatatc accatccact ggtgtaatga attccagcaa tgcttccgca
    31801 aaatcggtag taatccattt atgcgtcagt gcggaaccta atgcttcagg atgacctgtt
    31861 gttgccagtg tgccatcagc attaacacgc aaagtttcgc gctccagccc acgctgtatc
    31921 ccctttaacg cctgaggatg tttttccagc caggccagcg cctgtgatac gtccgggatc
    31981 aaattgacct cccgcctgtc aaaatcgttt taattagcat aactgtaatg gtgaccatat
    32041 gtgcaggcct acaattagtg ccaccacatc atgccctgaa cggtcgctgc tgcaactgca
    32101 acatagcgta acgctttacc aaggcacaaa aaaaagatta ccggccccca ggagatgcgc
    32161 atccatcccg ctaacagaca cagtaaatcg ccaaccacgg gcatccagct taataatagc
    32221 gtgactgcac catagcgttt cagccagccg gtagctttct cttgccagcg cgatgtttta
    32281 cgcaatggac agaaacgccc aaggataacg ttagttaacc ctccaaggct attacccatt
    32341 gttgctgtta aaactaaaac ccagggatga ctgatcccag aaagcaacat tgccaccagc
    32401 acgacttcgg agttgccggg taatagtgta gcgctgagaa aactactggc aaacaacgag
    32461 aaaagcgata acgcttcact cacagcaagc gaacatccac agcgtccatg cctgctgcac
    32521 gggccgcctg aataccgaaa tcggcatctt caaagaccac acactgcgtc ggttgcacgc
    32581 ccatacgctg cgcgcacaac aaaaatgtgt ctggcgcggg tttatggtgt ttgacgtgat
    32641 cggcagcgac gacggcgtca aaataacggc gtaatcccag gtgagccagc aatgcctcag
    32701 cgatggcgct ttcactcccc gttcctacag ccattgggcg acgaccatgc caacttttca
    32761 ccacttcaac aagaggaagc ggctcgacac tatccagcag catacttctt actgcttctg
    32821 ttttttcacg cgctaacgca tgcgggtcga gatcggcctg attcagctca ataattgcct
    32881 gagcaatacg ccaggtgggc gatccattaa gcgcaatcat cgcctgaaca tcgtactgaa
    32941 gaccgtagtg ccctaatact tcgcgccacg ctttacggtg cgtaggctcc gtatccagga
    33001 ttgtgccatc catatcaaaa attaaacctg cataacgctc gtacatggtc ttctcgcaag
    33061 tcgaaaaata tgacgctact ttagcgtaat tgcttgattt tgtcgctgat ggcgaaggag
    33121 aagaaaattc aggaaatgga taaagtagtt atttagaaat gagatatttt tgaaggaaat
    33181 tttttggaga agatggtgca tccgggagga tgactcactg gcgttcgccc ttcgggccgt
    33241 tgctaaagca acgttatcct tcctggtgcc tgcggttaat tccgctacct cttcgatatc
    33301 ttcattgctg aagatttcgg agaatatggt gcatccggga ggattcgaac ctccgaccgc
    33361 tcggttcgta gccgagtact ctatccagct gagctacgga tgcatcggga aa
//
LOCUS       DAZFBR010000047        32524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFBR010000047 DAZFBR010000000
VERSION     DAZFBR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.047"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            361..660
                     /locus_tag="ACHJZ2_002406"
     CDS             361..660
                     /locus_tag="ACHJZ2_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568495.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGGFPVTNIVLFLILIFFKKR"
     gene            complement(707..1594)
                     /gene="rarD"
                     /locus_tag="ACHJZ2_002407"
     CDS             complement(707..1594)
                     /gene="rarD"
                     /locus_tag="ACHJZ2_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO4568496.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1646..2113)
                     /gene="yigI"
                     /locus_tag="ACHJZ2_002408"
     CDS             complement(1646..2113)
                     /gene="yigI"
                     /locus_tag="ACHJZ2_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO4568497.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2278..3147
                     /gene="pldA"
                     /locus_tag="ACHJZ2_002409"
     CDS             2278..3147
                     /gene="pldA"
                     /locus_tag="ACHJZ2_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO4568498.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3280..5109
                     /gene="recQ"
                     /locus_tag="ACHJZ2_002410"
     CDS             3280..5109
                     /gene="recQ"
                     /locus_tag="ACHJZ2_002410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO4568499.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5173..5793
                     /gene="rhtC"
                     /locus_tag="ACHJZ2_002411"
     CDS             5173..5793
                     /gene="rhtC"
                     /locus_tag="ACHJZ2_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO4568500.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5855..6475)
                     /gene="rhtB"
                     /locus_tag="ACHJZ2_002412"
     CDS             complement(5855..6475)
                     /gene="rhtB"
                     /locus_tag="ACHJZ2_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO4568501.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6586..7608
                     /gene="pldB"
                     /locus_tag="ACHJZ2_002413"
     CDS             6586..7608
                     /gene="pldB"
                     /locus_tag="ACHJZ2_002413"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO4568502.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7616..8416
                     /gene="yigL"
                     /locus_tag="ACHJZ2_002414"
     CDS             7616..8416
                     /gene="yigL"
                     /locus_tag="ACHJZ2_002414"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO4568503.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8492..9391
                     /gene="bioP"
                     /locus_tag="ACHJZ2_002415"
     CDS             8492..9391
                     /gene="bioP"
                     /locus_tag="ACHJZ2_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO4568504.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9279..10232)
                     /gene="metR"
                     /locus_tag="ACHJZ2_002416"
     CDS             complement(9279..10232)
                     /gene="metR"
                     /locus_tag="ACHJZ2_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO4568505.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10468..12729
                     /gene="metE"
                     /locus_tag="ACHJZ2_002417"
     CDS             10468..12729
                     /gene="metE"
                     /locus_tag="ACHJZ2_002417"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO4568506.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12769..13584)
                     /gene="ysgA"
                     /locus_tag="ACHJZ2_002418"
     CDS             complement(12769..13584)
                     /gene="ysgA"
                     /locus_tag="ACHJZ2_002418"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO4568507.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13846..14607
                     /gene="udp"
                     /locus_tag="ACHJZ2_002419"
     CDS             13846..14607
                     /gene="udp"
                     /locus_tag="ACHJZ2_002419"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO4568508.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14748..16175
                     /gene="rmuC"
                     /locus_tag="ACHJZ2_002420"
     CDS             14748..16175
                     /gene="rmuC"
                     /locus_tag="ACHJZ2_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO4568509.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            16270..17025
                     /gene="ubiE"
                     /locus_tag="ACHJZ2_002421"
     CDS             16270..17025
                     /gene="ubiE"
                     /locus_tag="ACHJZ2_002421"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO4568510.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            17039..17644
                     /gene="ubiJ"
                     /locus_tag="ACHJZ2_002422"
     CDS             17039..17644
                     /gene="ubiJ"
                     /locus_tag="ACHJZ2_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO4568511.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            17641..19281
                     /gene="ubiB"
                     /locus_tag="ACHJZ2_002423"
     CDS             17641..19281
                     /gene="ubiB"
                     /locus_tag="ACHJZ2_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO4568512.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19360..19629
                     /gene="tatA"
                     /locus_tag="ACHJZ2_002424"
     CDS             19360..19629
                     /gene="tatA"
                     /locus_tag="ACHJZ2_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO4568513.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19633..20148
                     /gene="tatB"
                     /locus_tag="ACHJZ2_002425"
     CDS             19633..20148
                     /gene="tatB"
                     /locus_tag="ACHJZ2_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO4568514.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            20151..20927
                     /gene="tatC"
                     /locus_tag="ACHJZ2_002426"
     CDS             20151..20927
                     /gene="tatC"
                     /locus_tag="ACHJZ2_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO4568515.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20969..21751
                     /gene="tatD"
                     /locus_tag="ACHJZ2_002427"
     CDS             20969..21751
                     /gene="tatD"
                     /locus_tag="ACHJZ2_002427"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO4568516.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21748..22236)
                     /gene="rfaH"
                     /locus_tag="ACHJZ2_002428"
     CDS             complement(21748..22236)
                     /gene="rfaH"
                     /locus_tag="ACHJZ2_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO4568517.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22403..23896
                     /gene="ubiD"
                     /locus_tag="ACHJZ2_002429"
     CDS             22403..23896
                     /gene="ubiD"
                     /locus_tag="ACHJZ2_002429"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO4568518.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23942..24643
                     /gene="fre"
                     /locus_tag="ACHJZ2_002430"
     CDS             23942..24643
                     /gene="fre"
                     /locus_tag="ACHJZ2_002430"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO4568519.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24835..25998)
                     /gene="fadA"
                     /locus_tag="ACHJZ2_002431"
     CDS             complement(24835..25998)
                     /gene="fadA"
                     /locus_tag="ACHJZ2_002431"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO4568520.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(26008..28197)
                     /gene="fadB"
                     /locus_tag="ACHJZ2_002432"
     CDS             complement(26008..28197)
                     /gene="fadB"
                     /locus_tag="ACHJZ2_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO4568521.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28387..29718
                     /gene="pepQ"
                     /locus_tag="ACHJZ2_002433"
     CDS             28387..29718
                     /gene="pepQ"
                     /locus_tag="ACHJZ2_002433"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO4568522.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29718..30332
                     /gene="yigZ"
                     /locus_tag="ACHJZ2_002434"
     CDS             29718..30332
                     /gene="yigZ"
                     /locus_tag="ACHJZ2_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO4568523.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30371..31822
                     /gene="trkH"
                     /locus_tag="ACHJZ2_002435"
     CDS             30371..31822
                     /gene="trkH"
                     /locus_tag="ACHJZ2_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO4568524.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31834..32379
                     /gene="hemG"
                     /locus_tag="ACHJZ2_002436"
     CDS             31834..32379
                     /gene="hemG"
                     /locus_tag="ACHJZ2_002436"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO4568525.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7599 a         8176 c         8849 g         7900 t
ORIGIN      
        1 ccagaataaa actcaaagcc aggttgatat cattcaaagt tattaatatt gaccatgatg
       61 ttaatgtata gattatgaac tgaaagcgtg ataaacgctc aagcttggat gggtagaata
      121 tatttctgat ggatgatttg aacatttttt acattgtttt atatatataa ttgttttgaa
      181 tatatattac ctgaattatg tggtttgcat atatatatgc aatatagcag tctttgcatt
      241 gataactata cagtagtaat ttagagtgtg taagttttta ttgatggggt atattttaat
      301 taaatataat tggaatgtat ttttattcta taaacaatat cttgggtgaa tggattctat
      361 atgggaaatg gtaacttttg gcagttgttc ttgggtaaag aaccttacag caaaaaatct
      421 gattttgtta aggcatggtt attgtggtgg gctattacca tccttgttct atttatttta
      481 agcctgacta cgacattaaa tttaagaagt ataggtatag ggttaagttt tgggttttgt
      541 caaatccttg gtagatatac gatgtctcta ggatggagta agtactgggg agttttgggg
      601 ggatttcctg taacaaatat tgttcttttt cttatcttaa tttttttcaa aaaaagataa
      661 ataagtcagt gggtaggctc tttaaacggc ctacccatgt taattcttaa ctttttctac
      721 gctgcgtata aatcgcatcc atcacaaaaa ttgccagcgc cacccaaata aaggtgaaag
      781 tcaccatctt atcggcaccc ggtttttcac cataaaacgt cacagccagc aggaacatca
      841 gcgtcgggcc aatgtactgg aaaaagccta acgttgagag acgaaggcgc gtggcagcgg
      901 cggtaaaaca caacagcggt acggtagtga caataccggc ggcgatcagc agtaaattca
      961 gcgacatcgg gttttgcccc atatggctgg ttgagctgtc ggcaatagca aacaggtaaa
     1021 tcgccgccac gggcagcagc cacatggttt caattaacat gccggtttgt gcttctaccg
     1081 caatcttctt gcgcaccagg ccgtagaagg caaaactaaa tgccagcccc agcgcgataa
     1141 taggtagcga accaaaagtc cacagctgga ctaacacgcc acatatcgcc agaatcaccg
     1201 ccagccattg catccggcgg aatcgctcgc cgaggaaaat catccccagc acaatgttca
     1261 ccagcgggtt aataaagtaa ccaaggctcg cttccagcat atggtgattg ttcaccgccc
     1321 agataaacag tagccagttg ccaccaatca gcacggcaga gactgccagc ataaaaattt
     1381 tctgtggcgt ctgaatcagc gtttttaaat aggaccactg gcggcaaatg ctcatcagca
     1441 ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag gatctcatcg gcgggcacgt
     1501 agtaaatcaa cttgaagtac gctggggcta taccccaaat aaaataagcg gcaagagcga
     1561 gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt taattggtaa
     1621 cagcagttta cctgctttta tgtcttcaac ctaccatata ggtggcggtg gcactggcaa
     1681 tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac
     1741 gcaacaggct actggtagca gtaaaacgct cgcctctgcc tgggcgcaga taatcgacgc
     1801 gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt
     1861 cgtggcgggt taaggtactt cctacgcaca ccagaccggc ggcgacatcc agcgccgacg
     1921 caatgacccc gccgtgcaaa atgctttgcg cccagttgcc caccatcatt ggctgatttt
     1981 taaaggccag ctgtgcgaac tctttttcgt aacgctccag ttccatcccc aatgcgcggt
     2041 taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca
     2101 gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgattc
     2161 tttgttgttg gtttctactt taggaaggga taactaacgg caatggagtt aagtatgtaa
     2221 aataccccgc agaaaaacat tcaccttatc aataattcgt tacggagaac acgaccgatg
     2281 cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa
     2341 gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat
     2401 atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatc
     2461 tacacccaaa ccagcgatct gaacaaagaa gcgattgcca gttacgactg ggcggaaaat
     2521 gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccgctgtg gcgtgggatt
     2581 ttaggcccga actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc
     2641 aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt
     2701 tttgccaccg attaccgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac
     2761 cacgactcta acgggcgttc agacccgacc tcccgcagct ggaaccgcct ttatactcgc
     2821 ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat
     2881 actgacgata acccggatat caccaaatat atgggttact accagcttaa aatcggctat
     2941 cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca actggaacac cggctacggc
     3001 ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt
     3061 tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgtg
     3121 ggggttatgc taaacgattt gttttgatga acggttgagt ggttggcaaa tctggaatcc
     3181 agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga
     3241 aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg
     3301 aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc
     3361 cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct ggtcgtcatg
     3421 cccaccggtg gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt
     3481 accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca actgcaagcc
     3541 aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca acttgaagtg
     3601 atgacaggct gccgcaccgg gcaaattcgt ctgctttata tcgccccgga acgcctgatg
     3661 ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc cgttgatgaa
     3721 gcgcactgta tctcccaatg gggccacgat ttccgcccgg aatatgccgc gctcggtcag
     3781 ttgcgccagc ggttcccgac gctgccgttt atggcgctga ccgccacagc cgacgacacc
     3841 acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc cgctgattca aatcagcagt
     3901 tttgaccgtc cgaatattcg ctacatgctg atggagaaat tcaaaccgct cgatcagttg
     3961 atgcgctacg tgcaggaaca gcgcggtaag tccggcatta tctactgcaa cagccgggcg
     4021 aaagtagaag acaccgctgc gcgcctgcaa agcaagggta ttagcgcggc ggcctatcat
     4081 gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga aattccagcg cgatgacctg
     4141 caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc
     4201 gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc
     4261 gggcgtgatg gcctgcctgc ggaagcgatg ttgttttacg atccagctga tatggcgtgg
     4321 ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc aggatatcga gcgccacaaa
     4381 ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc gtcgtctggt attgctgaac
     4441 tattttggcg aagggcgtca ggagccgtgc gggaactgcg atatctgcct cgatccgccg
     4501 aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat
     4561 cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc
     4621 cgcgactatg gtcatgacaa actgaaagtc tatggcatgg gccgtgataa aagccatgaa
     4681 cattgggtga gcgtgatccg ccagctgatt cacctcggcc tggtgacgca aaatattgcc
     4741 cagcattctg ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct
     4801 ttacaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc
     4861 ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc
     4921 gatgaaagta atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct
     4981 gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag
     5041 ctggaacgct ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac
     5101 gaagagtagt cagcagcata aaaaagtgcc aggatgaaga ctccgtaaac gtttcccccg
     5161 cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt
     5221 atgagtcccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa
     5281 gaagcgatga tgggcgtgct gggtattacc tgcggcgtta tggtttgggc agggattgcg
     5341 ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct gattatggtt
     5401 ggcggtggcc tgtatctctg ctggatgggt taccagatgc tgcgcggcgc gctgaaaaaa
     5461 gaggcggttt ctgcacctgc gccacaggtc gagctggcga aaagtgggcg cagtttcctg
     5521 aaaggtttac tgaccaatct cgctaatccg aaagcgatta tctactttgg ctcggtgttc
     5581 tcattgtttg tcggtgataa cgttggcact accgcgcgct ggggcatttt cgcgttgatt
     5641 attgtcgaaa cgctggcgtg gtttactgtg gtggccagcc tgtttgccct gccgcaaatg
     5701 cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc gttatttgcc
     5761 ggatttggca ttcatttgat tatttcgcgg taatgccaga cgcgtcttca gagtaagtcg
     5821 gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc gatgctaaca
     5881 gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc atctgctttg
     5941 gtcctttaat ccatagagca atccgttgag caagggtggc gtaaccgatc atcacaataa
     6001 tatcgaccac aatagtggtg acaccgagca cgatatactg catcagttgc ggctgttgcg
     6061 gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga ttggtgagat
     6121 tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc agcgatttaa
     6181 gatcaattgc gccagcggcg cgccattgct gtattcccag ccaaatcaag taagccgcgc
     6241 ctgcccactt caacacttca aacgcaatca ctgagcggga aaatagcgtc cccaacccca
     6301 cgccaaccag cacaatatga atcgccagtc cggtctgaag cccggcaata gacgccaccg
     6361 cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca gagcctggcg
     6421 acaggcttaa aatgattgat gtcagtaggt aggcaaacca ccattctaag gtcatgatga
     6481 actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca
     6541 cgctaaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa
     6601 aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc gctgactgat
     6661 ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc ggtgcgcttt
     6721 gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg gcgtattgag
     6781 agttacgtaa aatatgcgga actggcctac gatttgttcc atttggggtt tgatgtctta
     6841 atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg
     6901 catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca acaggaggtc
     6961 cagcccggtc cgtggcgtaa acgctatata ctggcacatt cgatgggcgg tgcgatctcc
     7021 acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg
     7081 tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa ctgggccgaa
     7141 gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg cgcgttgccg
     7201 tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt acgcttctat
     7261 gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg cgaaagtatt
     7321 ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgccaac gcttctcttg
     7381 caggctgaag aggaacgcgt ggtggataac cgcattcatg accgtttttg tgaactccgc
     7441 accgccgcgg gccatcctgt cgaaggggga cgaccgctgg taattaaagg tgcgtaccat
     7501 gagatccttt ttgaaaagga cgcaatgcgc tcagttgcgc tccacgccat cgttgatttt
     7561 ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat ttcttatgta
     7621 ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata cattatcccc
     7681 ttacgccaaa gaaaccctga agctgctcac tgcgcgcggc atcaactttg tgtttgcgac
     7741 cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatta agtcttacat
     7801 gattacctcc aatggtgcgc gcgttcacga tctggatggt aatctgattt ttgctcataa
     7861 cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc cggacatcat
     7921 tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg aagagatgcg
     7981 cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag cctggattac tggagccgga
     8041 aggcgtcagc aaagtgttct tcacctgcga ttcccatgaa caactgctgc cgctggagca
     8101 ggcgattaac gctcgttggg gcgatcgcgt caacgtcagt ttctctacct taacctgtct
     8161 ggaagtgatg gcgggcggcg tttcaaaagg ccatgcgctg gaagcggtgg cgaagaaact
     8221 gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgatg ccgaaatgct
     8281 gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga aagaccttca
     8341 tcccgagctg gaagtgattg gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa
     8401 actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac ttcgctacaa
     8461 tggatactcg ttaatcaaag agttttccat tgtggcgcta cttatcatca ccacgattct
     8521 gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg atagctattt
     8581 tgcggtgctg gtgcgcgttg gcctggcggc actcgttttt ctgccgtttc tgcgtacccg
     8641 tggcaatagc ctgaaaacag tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt
     8701 gatgtatatg ctgagtttcc gcgcttatct ctacctgacg gtttccgaac tgctgctgtt
     8761 caccgtactg acgccgctct acatcacgct gatttatgac atcatgagta agcgccgtct
     8821 gcgctgggga tatgctttta gtgccttgtt ggcggtgatt ggtgccggga ttattcgcta
     8881 tgatcaggtc accgaccatt tctggactgg cttgttgctg gtgcaactct ccaatatcac
     8941 ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga tgccgcagca
     9001 taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg catggttctt
     9061 gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc tggtgtttct
     9121 tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcaa cccaagtaga
     9181 cgccggaacg ctgggcatta tgaataatat gcacgttccg gcagggctgc tggttaacct
     9241 ggctatctgg caccaacagc cgcactggcc aacgtttatt acaggcgcgc tggtgatcct
     9301 ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga
     9361 tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg taactggctg acgctgctcg
     9421 ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt tttggtcacc
     9481 accagaccct ggcgctcaaa actctctact acccaatgtg gtagcgcggc aatacccatc
     9541 cgcgcggcaa ccatctgaat caacaataag gtgttatcga cgcttttcag cgacgggcta
     9601 acgcctgcgg gctggagaaa atgccgccag acatccagtc ggctacgctg caccggataa
     9661 atcaacagcg tttcgctggc gagatcttcc ggcgtgattc gcgttttcgc cgccagtgga
     9721 tggtcaggtg ctaacaccag acgcacttca tagtcgaaca tcggcgaata atgcaggcca
     9781 ctacgcggca gaatatcgga ggtcatcacc agatccagct cgccctgctg caacgcaggc
     9841 tgcgggtcaa acgttacgcc cgatttaaaa tccatctcca cctgcggcca gttcttatgg
     9901 aaattttcta atgcgggcgt cagccactga atacagctat ggcactcaat ggcaatgcgc
     9961 aggcgcgtct gttgcggttc attgcaggct tgcagcgcct ggctaatttg cggcagcacc
    10021 tggtttgcca gttgcaacag gatttccccc tgtggagtaa agcgtagcgg ctggctctta
    10081 cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg agacagggcg
    10141 gattgcgttt gatgcaacgt cgccgcagcg gctgcgagag agccgcagtt ccgcaacgct
    10201 tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg catgaataat
    10261 ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc tggacggcta
    10321 aaatccttcg tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc cggtcactta
    10381 cttcagtaag ctcccgggga tgaataaact tgccgccttc cctaaattca aaatccatag
    10441 gatttacata taattagagg aagaaaaatg acaatattga atcacaccct cggtttccct
    10501 cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaga gttattgggc ggggaactcc
    10561 acgcgtgaag aactgctggc ggtagggcgt gaattgcgtg ctcgtcactg ggatcaacaa
    10621 aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgtactg
    10681 accaccagtc tgctgctggg taacgttccg gcgcgtcatc agaacaaaga tggttcggta
    10741 gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg
    10801 gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc ggagttcgtt
    10861 aaaggccaac agttcaaact gacctggacg cagctgctgg acgaagtgga cgaggcgctg
    10921 gcgctgggcc acaaggtgaa acctgtgctg ctggggccga ttacctggct gtggctgggg
    10981 aaagtgaaag gtgaacagtt tgaccgcctg agcctgctga acgacattct gccggtttat
    11041 cagcaagtgc tggcagaact ggcgaaacgt ggcatcgagt gggtacagat tgatgaaccc
    11101 gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg
    11161 ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc
    11221 gacacgatta ctgcgctgcc tgttcagggg ctgcatgtgg accttgtaca tggtaaagat
    11281 gacgttgttg aactgcacaa gcgcctgcct tctgactggc tgctgtctgc gggtctgatc
    11341 aatggtcgta acgtctggcg cgccgatctt actgagaaat atgtgcaaat taaggacatt
    11401 gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac
    11461 ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa
    11521 aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg
    11581 gcagagtgga gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca taatccggcg
    11641 gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa
    11701 gtgcgtgctg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt
    11761 ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat
    11821 ctcgacgcca ataactaccg cacgggcatt gcggaacata tcaggcaggc cattgttgaa
    11881 caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg
    11941 gaatactttg gcgagcacct cgacgggttt gtctttacgc aaaacggttg ggtacagagc
    12001 tacggttccc gctgcgtgaa gccaccgatt gttattggtg acgttagtcg cccggcaccg
    12061 attaccgtgg agtgggcgaa gtatgcgcaa tctatgactg ataaaccggt gaaagggatg
    12121 ctgactggcc cggtgaccat tctctgctgg tcgttcccgc gtgaagatgt cagccgtgaa
    12181 accatcgcca aacaaattgc gctggcgctg cgtgatgaag tggcggacct ggaagccgca
    12241 ggaattggca tcatccagat tgatgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt
    12301 agcgactggg atgcatatct ccagtggggc gtggaggcct tccgtatcaa cgccgccgtg
    12361 gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg
    12421 gattcgattg cggcactgga cgcagacgtc atcaccatcg aaacctcgcg ttctgacatg
    12481 gagttgctgg agtcgttcga agagtttgat tatccgaatg aaatcggtcc aggcgtctat
    12541 gacattcact cgccaaacgt accgagcgtg gaatggattg aagccttgct gaagaaagcg
    12601 gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc
    12661 ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaacttgcgt
    12721 cgggggtaaa atccaaaccg ggtggtaata ccacccggtc ttttctcatt acagcgactt
    12781 cttcccacca tactgcttaa accattccag catacgctgc cagccatctt ctgcagatgc
    12841 ggcatgatag ctcgggcgat aatcagcgtt gaatgcatgc ccggcgtccg ggtacacgat
    12901 aatctctgct ttcgcattag cagcccgcag cgcctggcgc atggtttcaa cgctctcctg
    12961 cggaatactg ttatccagac cgccatataa gccgagaacc ggcgcgttaa gatcggttgc
    13021 gatatcaaca ggttgtttcg gtgaattcag cgacttatcg cccgtcagtt tgccgtacca
    13081 cgccactgcg gcttttagct gtggattatg cgcggcatac agccaagtga tacgtccacc
    13141 ccagcagaat ccggtgatca ttaaacgatg aacatcgccg ccgttgcggg aagcccaact
    13201 ggcgacatga tcgagatcgg ccagcacctg cgagtcaggc acttttgcta ccagaccgct
    13261 aagcaacgtg gggatatcgg caaaatcatt cggatcgcct tcgcggaagt aaagttcagg
    13321 tgcgatagcc agatacccct ccagcgccag acggcgacaa atgtcgcgga tatgttcatg
    13381 cacgccaaaa atttcctgca ctacaatgac cactggcagt gggccatcgc tttgctttgg
    13441 tctggcatgg taagcaggca tgttatcccc ttgtgaaggg atagaggtga agcccgccac
    13501 aatcgcgtcg tccggggtct gaacgatggt cgaagcgaga ggcgatgcag caggtgcaaa
    13561 tccagattgt tgtgttgttg ccatggtatt ctccgtaccc ttataaaaat gttgcgcaat
    13621 gttaactata gtcagcatgc aacaaatcac attgcctgaa tcggctcatc ttttatgcag
    13681 tcctgcagaa tgaagagtga tttatgtgat ttgcatcacc tttggtgggt aaatttatgc
    13741 aacgcatttg cgtcatggtg atgagtatca cgaaaaaatg ttaaaccctt cggtaaagtg
    13801 tctttttgct tcttctgact aaaccgattc acagaggagt tgtatatgtc caagtctgat
    13861 gtttttcatc tcggcctcac taaaaacgat ttacaagggg ctacgcttgc catcgtccct
    13921 ggcgacccgg atcgtgtgga aaagatcgcc gcgctgatgg ataagccggt taagctggca
    13981 tctcaccgcg aattcactac ctggcgtgca gagctggatg gtaaacctgt tatcgtctgc
    14041 tctaccggta tcggcggccc gtctacctct attgctgttg aagagctggc acagctgggc
    14101 attcgcacct tcctgcgtat cggtacaacg ggcgctattc agccgcatat taatgtgggt
    14161 gatgtcctgg ttaccacggc gtctgtccgt ctggatggcg cgagcctgca cttcgcaccg
    14221 ctggaattcc cggctgtcgc tgatttcgaa tgtacgactg cgctggttga agctgcgaaa
    14281 tccattggcg cgacaactca cgttggcgtg acagcttctt ctgatacctt ctacccaggt
    14341 caggaacgtt acgatactta ctctggtcgc gtagttcgtc actttaaagg ttctatggaa
    14401 gagtggcagg cgatgggcgt aatgaactat gaaatggaat ctgcaaccct gctgaccatg
    14461 tgtgcaagtc agggcctgcg tgccggtatg gtagcgggtg ttatcgttaa ccgcacccag
    14521 caagagatcc cgaatgctga gacgatgaaa caaactgaaa gccaagcggt gaaaatcgtg
    14581 gtggaagcgg cgcgtcgtct gctgtaattc tcttctcctg tctgaaggcc gacgcgttcg
    14641 gccttttgta tttttgcgta gcgcctcgca ggaaatgcct ttccaactgg acgtttgtac
    14701 agcacaattc tattttgtgc gggtaagttg ttgcgtcagg aggcgttgtg gatttctcaa
    14761 tcatggttta cgcagttatt gcgttggtgg gtgtggcaat tggctggctg tttgccagtt
    14821 atcaacatgc gcagcaaaaa gccgagcaat tagctgaacg tgaagagatg gtcgcggagt
    14881 taagcgcggc aaaacaacaa attacccaaa gcgagcactg gcgtgcagag tgcgagttac
    14941 tcaataacga agtgcgcagc ctgcaaagta ttaacacctc tctggaggcc gatctgcgtg
    15001 aagtaaccac gcggatggaa gccgcacagc aacatgctga cgataaaatt cgccagatga
    15061 ttaacagcga gcagcgcctc agtgagcagt ttgaaaacct cgccaaccgt atttttgagc
    15121 acagcaatcg ccgggttgat gagcaaaacc gtcagagtct gaacagcctg ttgtcgccgc
    15181 tacgtgaaca actggacggt ttccgccgtc aggttcagga cagcttcggt aaagaagcac
    15241 aagaacgcca taccctgacc cacgaaattc gcaatctcca gcaactcaac gcgcaaatgg
    15301 cccaggaagc gatcaacctg acgcgcgcgc tgaaaggcga caataaaacc cagggcaact
    15361 ggggcgaggt agtattgacg cgggtgctgg aggcttccgg tctgcgtgaa gggtatgaat
    15421 atgaaaccca ggtcagcatc gaaaatgacg cccgctcgcg gatgcagccg gatgtcatcg
    15481 tgcggctgcc gcagggaaaa gatgtggtga tcgacgccaa aatgacgctg gtcgcctatg
    15541 aacgctattt taacgccgaa gacgactaca cccgcgaaag cgcgctacag gaacatatcg
    15601 cgtcggtgcg taaccatatc cgtttgctgg gacgcaaaga ttatcaacag ctgccggggc
    15661 tgcgaactct ggattacgtg ctgatgttta ttcccgttga acccgctttt ttactggcgc
    15721 ttgaccgcca gccggagctg atcaccgaag cgttgaaaaa caatatcatg ctggttagcc
    15781 cgactacgct gctggtggcg ctgcgcacta tcgccaacct gtggcgttat gagcatcaaa
    15841 gccgcaacgc ccagcaaatc gccgatcgtg ccagcaagct gtacgacaag atgcgtttgt
    15901 tcatcgatga catgtccgcg attggtcaaa gtctcgacaa agcgcaggat aactatcggc
    15961 aggcaatgaa aaaactctct tcagggcgcg gaaatgtgct ggcgcaggca gaagcgtttc
    16021 gcggtttagg agtagaaatt aaacgcgaga ttaatccgga tttggctgaa caggcggtga
    16081 gccaggatga agagtatcga cttcggtcgg ttccggagca gccgaatgat gaagcttatc
    16141 aacgcgatga tgaatataat cagcagtcgc gctagcccat tgggagtagt taagccgggt
    16201 agaaatctag ggcatcgacg cccaatctgt tacacttctg gaacaatttt ttgatgagca
    16261 ggcattgaga tggtggataa gtcacaagaa acgacgcact ttggttttca gaccgtcgcg
    16321 aaggaacaaa aagcggatat ggtcgcccac gttttccatt ccgtggcatc aaaatacgat
    16381 gtcatgaatg atttgatgtc atttggtatt catcgtttgt ggaagcgatt cacgattgat
    16441 tgcagcggcg tacgccgtgg gcagaccgtg ctggatctgg ctggtggcac cggcgacctg
    16501 acagcgaaat tctcccgcct ggtcggagaa actggcaaag tggtccttgc tgatatcaat
    16561 gaatccatgc tcaaaatggg ccgcgagaag ctgcgtaata tcggtgtgat tggcaacgtt
    16621 gagtatgttc aggcgaacgc tgaggcgctg ccgttcccgg ataacacctt tgattgcatc
    16681 accatttcgt ttggtctgcg taacgtcacc gacaaagata aagcactgcg ttcaatgtat
    16741 cgcgtgctga aacccggcgg ccgcctgctg gtgcttgagt tctcgaagcc aattatcgag
    16801 ccgctgagca aagcctatga tgcatactcc ttccatgtgc tgccgcgtat tggctcactg
    16861 gtcgcgaacg acgccgacag ctaccgttat ctggcagaat ccatccgtat gcatcccgat
    16921 caggataccc tgaaagccat gatgcaggat gccggattcg aaagtgtcga ctactacaat
    16981 ctgacggcag gggttgtggc gctgcatcgt ggttataagt tctgacagga gaccggaaat
    17041 gccttttaaa cctttagtga cggcaggaat tgaaagtctg ctcaacacct tcctgtatcg
    17101 ctcacccgcg ctgaaaacgg cccgctcgcg tctgttgggc aaagtgctgc gcgtggaggt
    17161 aaaaggcttt tcgacgtcat tgattctggt gttcagcgaa cgccaggttg atgtactggg
    17221 cgaatgggca ggcgatgctg actgcaccgt tatcgcctac gccagtgtgt tgccgaaact
    17281 tcgcgatcgc cagcagcttg ccgccctgat tcgcagtggt gagctggaag tgcagggcga
    17341 tattcaggtg gtgcaaaact tcgttgcgct ggcaggtctg gcagagttcg accctgcgga
    17401 actgctggcc ccttataccg gtgatatcgc cgctgaagga atcagcaaag ccatgcgcgg
    17461 aggcgcaaag ttcctgcatc acggcattaa gcgccagcaa cgttatgtgg cggaagccat
    17521 tactgaagag tggcgtatgg cacccggtcc gcttgaagtg gcctggtttg cggaagagac
    17581 ggctgccgtc gagcgtgctg ttgatgccct gatcaaacgg ctggaaaaac tggaggctaa
    17641 atgacgccag gtgaagtacg gcgcctatat ttcatcattc gcactttttt aagctacgga
    17701 cttgatgaac tgatccccaa aatgcgtatc accctgccgc tacggctatg gcgatactca
    17761 ttattctgga tgccaaatcg gcataaagac aaacttttag gtgagcgact acgactggcc
    17821 ctgcaagaac tggggccggt atggatcaag ttcgggcaaa tgttatcaac ccgccgcgat
    17881 ctttttccgc cgcatattgc cgatcagctg gcgttattgc aggacaaagt tgctccgttt
    17941 gatggcaagc tggcgaagca gcagattgaa gctgcaatgg gcggcttgcc ggtagaagcg
    18001 tggtttgacg attttgaaat caagccactg gcttctgctt ctatcgccca ggttcatacc
    18061 gcgcgattga aatcgaatgg taaagaggtg gtgattaaag tcatccgccc ggatattttg
    18121 ccggtcatta aagcagacct gaaacttatc taccgtctgg ctcgctgggt gccgcgtttg
    18181 ctgccggatg gtcgccgtct acgcccaacc gaagtggtgc gcgagtacga aaagaccttg
    18241 attgatgaac tgaatttgct gcgggaatct gccaacgcca ttcagcttcg gcgaaatttt
    18301 gaagacagcc cgatgctcta catcccggag gtttaccctg actattgtag tgaagggatg
    18361 atggtgatgg agcgtattta cggcattccg gtgtctgatg ttgcggcgct ggagaaaaac
    18421 ggcaccaaca tgaaattgct ggcggaacgc ggcgtgcagg tgttcttcac tcaggtcttt
    18481 cgcgacagct ttttccatgc cgatatgcac cctggcaaca tcttcgtaag ctatgaacac
    18541 ccggaaaacc cgaaatatat cggcattgat tgcgggattg ttggctcgct aaacaaagaa
    18601 gataaacgct atctggcaga aaactttatt gccttcttta atcgcgacta tcgcaaagtg
    18661 gcagagctac acgtcgattc tggctgggtg ccaccagata ccaacgttga agagttcgaa
    18721 tttgctattc gtacggtctg tgaacctatc tttgagaaac cgctggccga aatttcgttt
    18781 ggacatgtac tgttaaatct gtttaatacg gcgcgtcgct tcaatatgga agtgcagccg
    18841 caactggtgt tactccagaa aaccctgctc tacgtcgaag gggtaggacg ccagctttat
    18901 ccgcaactcg atttatggaa aacggcgaag cctttcctgg agtcgtggat taaagatcag
    18961 gtcggtattc ctgcgctggt gagagcattt aaagaaaaag cgccgttctg ggtcgaaaaa
    19021 atgccagaac tgcctgaatt ggtttacgac agtttgcgcc agggcaagta tttacagcac
    19081 agtgttgata agattgcccg cgagcttcag tcaaatcatg tacgtcaggg acaatcgcgt
    19141 tattttctcg gaattggcgc tacgttagta ttaagtggca cattcttgtt ggtcagccga
    19201 cctgaatggg ggctgatgcc cggctggtta atggcaggtg gtctgatcgc ctggtttgtc
    19261 ggttggcgca aaacacgctg attttttcat cgctcaaggc gggccgtgta acgtataatg
    19321 cggctttgtt taatcatcat ctaccacaga ggaacatgta tgggtggtat cagtatttgg
    19381 cagttattga ttattgccgt catcgttgta ctgctttttg gcaccaaaaa gctcggctcc
    19441 atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa aagcaatgag cgatgatgaa
    19501 ccaaagcagg ataaaaccag tcaggatgct gattttactg cgaaaactat cgccgataag
    19561 caggcggata cgaatcagga acaggctaaa acagaagacg cgaagcgcca cgataaagag
    19621 caggtgtaat ccgtgtttga tatcggtttt agcgaactgc tattggtgtt catcatcggc
    19681 ctcgtcgttc tggggccgca acgactgcct gtggcggtaa aaacggtagc gggctggatt
    19741 cgcgcgttgc gttcactggc gacaacggtg cagaacgaac tgacccagga gttaaaactc
    19801 caggagtttc aggacagtct gaaaaaggtt gaaaaggcga gcctcactaa cctgacgccc
    19861 gaactgaaag cgtcgatgga tgaactacgc caggccgcgg agtcgatgaa gcgttcctac
    19921 gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca ccatccataa cccggtggtg
    19981 aaagataatg aagctgcgca tgagggtgta acgcctgccg ctgcacaaac gcaggccagt
    20041 tcgccggaac agaagccaga aaccacgcca gagccggtgg taaaacctgc tgcggacgct
    20101 gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata aaccgtaaac atgtctgtag
    20161 aagatactca accgcttatc acgcatctga ttgagctgcg taagcgtctg ctgaactgca
    20221 ttatcgcggt gatcgtgata ttcctgtgtc tggtctattt cgccaatgac atctatcacc
    20281 tggtatccgc gccattgatc aagcagttgc cgcaaggttc aacgatgatc gccaccgacg
    20341 tggcctcgcc gttctttacg ccgatcaagc tgacctttat ggtgtcgctg attctgtcag
    20401 cgccggtgat tctctatcag gtgtgggcat ttatcgcccc agcgctgtat aagcatgaac
    20461 gtcgcctggt ggtgccgctg ctggtttcca gctctctgct attttatatc ggcatggcgt
    20521 tcgcctactt tgtggtcttt ccgctggcat ttggcttcct tgccaatacc gcgccggaag
    20581 gggtgcaggt atccaccgac atcgccagct atttaagctt cgttatggcg ctgtttatgg
    20641 cgtttggtgt ctcctttgaa gtgccggtgg caattgtgct gctgtgctgg atggggatta
    20701 cctcgccaga agacttacgc aaaaaacgcc cgtatgtgct ggttggtgca ttcgttgtcg
    20761 ggatgttgct gacgccgccg gatgtcttct cgcaaacgct gttggcgatc ccgatgtact
    20821 gtctgtttga aatcggtgtc ttcttctcac gcttttacgt tggtaaaggg cgaaatcggg
    20881 aagaggaaaa cgacgctgaa gcagaaagcg aaaaaactga agaataaatt caaccgcccg
    20941 tcagggcggt tgtcatatgg agtacaggat gtttgatatc ggcgttaatt tgaccagttc
    21001 gcaatttgcg aaagaccgtg atgatgttgt agcgcgcgct tttgacgcgg gagttaatgg
    21061 gctactcatc accggcacta acctgcgtga aagccagcag gcgcaaaagc tggcgcgtca
    21121 gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac gacagcagcc agtggcaagc
    21181 tgcgactgaa gaagcgatta ttgagctggc cgcgcagcca gaagtggtgg cgattggtga
    21241 atgtggtctc gactttaacc gcaacttttc gacgccggaa gagcaggaac gcgcttttgt
    21301 tgcccagcta cgcattgccg cagatttaaa catgccggta tttatgcact gtcgcgatgc
    21361 ccacgagcgg tttatgacat tactggagcc gtggctggat aaactgcctg gtgcggttct
    21421 tcattgcttt accggcacac gcgaagagat gcaggcgtgc gtggcgcatg gaatttatat
    21481 cggcattacc ggttgggttt gcgatgaacg acgcggactg gagctgcggg aacttttgcc
    21541 gttgattccg gcggaaaaat tactgatcga aactgatgcg ccgtatctgc tccctcgcga
    21601 tctcacgcca aagccatcat cccggcgcaa cgagccagcc catctgcccc atattttgca
    21661 acgtattgcg cactggcgtg gagaagatgc cgcatggctg gctgccacca cggatgctaa
    21721 tgtcaaaaca ctgtttggga ttgcgtttta gagtttgcgg aactcggtgt tcttcacact
    21781 gtgcttaatc tctttattaa taagattaag caatagcatg gagcgagcct caccatcggg
    21841 ttcggtgaaa atggcctgaa agccttcgaa cgcgccttcg gtaataatca ccttatcacc
    21901 cggataaggg gttgccggat cgacaatgtc tttcggttta tataccgata gctgatgaat
    21961 caccgccgat gggactatcg ctggcgacgc gccaaagcgc acgaagtggc tgacaccgcg
    22021 ggtcgcgttg atagtcgtgg tatgaatcac ttctggatca aattccacaa acaggtagtt
    22081 ggggaacaat ggctcactga ctgcagtacg tttgccacgc acgatttttt ccagggtgat
    22141 catcggtgcc aggcaattca cagcctgtct ttcgaggtgt tcctgggcac gttgaagttg
    22201 cccgcgcttg cagtacagta aataccagga ttgcataatg actcttatcc gtttaatcgg
    22261 ggcgcaagga tagcaaaagc tttacgctaa gttaattata ttccccggtt tgcgttatac
    22321 cgtcagagtt cacgctaatt taacaaattt acagcatcgc aaagatgaac gccgtataat
    22381 gggcgcagat taagaggcta caatggacgc catgaaatat aacgatttac gcgacttctt
    22441 gacgttgctt gaacagcagg gtgagctaaa acgtatcacg ctcccggtgg acccgcatct
    22501 ggaaatcact gaaattgctg accgcacgct gcgtgctggt gggcctgcgc tgttgtttga
    22561 aaaccctaaa gggtactcaa tgccggtgct gtgcaacttg ttcggtacgc caaagcgcgt
    22621 agcgatgggt atgggccagg aagatgtttc agcactgcgt gaagtcggta aattattagc
    22681 atttctgaaa gaaccagagc cgccaaaagg ttttcgcgat ctgtttgata agctgccgca
    22741 gtttaagcag gtgttaaaca tgccgacaaa gcgactgcgc ggtgcaccct gccaacaaaa
    22801 aatcgtctct ggcgatgacg tcgatctcaa ccgtattccc attatgacct gttggccgga
    22861 agatgccgcg ccgctgatta catgggggct aaccgttaca cgtggccctc ataaagagcg
    22921 acagaatctg ggcatttatc gccagcaact gattggtaaa aacaagctga ttatgcgttg
    22981 gctgtcgcat cgcggcggcg cgctggatta tcaggagtgg tgtgcggcgc atccaggtga
    23041 acgtttcccg atctctgtgg cgttgggcgc tgatccggca accattctcg gtgcagtcac
    23101 accagtacca gatactttgt cggaatacgc ctttgccgga ttgctacgtg gcaccaaaac
    23161 cgaagtagtg aagtgtattt ccaatgatct cgaagtgccc gccagtgcgg agattgtgct
    23221 ggaagggtat atcgaacaag gcgaaatggc gccagaagga ccgtatggtg accacactgg
    23281 ttactataac gaagtcgata gtttcccggt atttaccgtg acgcatatta cccagcgtga
    23341 agatgcgatt taccattcca cctataccgg gcgtccgcca gatgaacccg cggtactggg
    23401 agtggcgttg aacgaagtat ttgttcccat tctgcaaaag cagttcccgg aaattgtcga
    23461 tttttacctg ccgccggaag gctgctctta tcgcctggcg gtagtgacaa tcaaaaaaca
    23521 gtacgccgga cacgcgaagc gcgtcatgat gggcgtctgg tcgttcttac gccagtttat
    23581 gtacactaaa tttgtgatcg tttgcgatga tgacgttaac gcacgcgact ggaacgatgt
    23641 gatttgggcg attaccaccc gtatggaccc agcgcgggat actgttctgg tagaaaatac
    23701 gcctattgat tatctggatt ttgcctcgcc tgtctccggg ctgggttcaa aaatggggct
    23761 ggatgccacg aataaatggc cgggggaaac ccagcgtgaa tggggacgtc ccatcaaaaa
    23821 agatccagat gttgtcgcac atattgacgc catctgggat gaactggcta tttttaacaa
    23881 cggtaaaagc gcctgatgcg cgtttgtttt gccctattta tcgatccgac agagaaagcg
    23941 catgacaacc ttaagctgta aagtgacctc ggtagaagct atcacggata ccgtatatcg
    24001 tgtccgcatc gtgccagacg cggccttttc ttttcgtgct ggtcagtatt tgatggtagt
    24061 gatggatgag cgcgacaaac gtccgttctc aatggcctcg acgccggatg aaaaagggtt
    24121 tatcgagctg catattggcg cttctgaaat caacctttac gcgaaagcag tcatggaccg
    24181 catcctcaaa gatcatcaaa tcgtggtcga tattccccac ggagaagcgt ggctgcgcga
    24241 tgatgaagag cgtccgatga ttttgattgc gggcggcacc gggttctctt atgcccgctc
    24301 gattttgctg acagcgttgg cgcgtaaccc aaaccgtgat atcaccattt actggggcgg
    24361 gcgtgaagag cagcatctgt atgatctctg cgagcttgag gcgctttcgt tgaagcatcc
    24421 tggtctgcaa gtggtgccgg tggttgaaca accggaagcg ggctggcgtg ggcgtactgg
    24481 caccgtgtta acggcggtat tgcaggatca cggtacgctg gcagagcatg atatctatat
    24541 tgccggacgt tttgagatgg cgaaaattgc ccgtgacctg ttttgcagtg agcgtaatgc
    24601 gcgggaagat cgcctgtttg gcgatgcgtt tgcatttatc tgagatataa aaaaacccgc
    24661 ccctgacagg cgggaagaac ggcaactaaa actttattca gtggcattta gatctatgtc
    24721 gcatccggca ttcggcacac gatgcctgat gcgacgctgg cgcgtcttat caggcctaca
    24781 ttggtgccgg atcggtagac cggataaggc gttcacgccg catccggcaa gtggttaaac
    24841 ccgctcaaac accgtcgcaa taccctgacc cagaccgata cacatcgtcg ccagaccaaa
    24901 ctgaacgtct ttgtgttcca tcagattcag cagcgtggtg ctgatacgcg caccggaaca
    24961 acccagcgga tgacccagcg cgatcgcgcc accgttgagg ttgatcttct cgtcaatctg
    25021 ctcaattagt cccagatctt taatacatgg caggatctgc gcggcaaagg cttcattcat
    25081 ttcaaacacg ccgatatcac tgacagaaag ccccgctttt ttcagcgcca gcttcgaggc
    25141 cggaaccggg ccgtaaccca tgatcgatgg gtcacaacca acgaccgcca tcgaacgcac
    25201 acgagcgcgc ggcttaagac ctaattcatg ggcgcggctt tcactcatca ccagcatggc
    25261 agctgcgcca tcggaaagtg cagaagatgt gcccgccgtt accgtaccgt ttactggatc
    25321 aaacgccgga cgcagcgtgg cgagggcttc cacggtggtt tccgggcgaa tcacttcgtc
    25381 gtaattaaac tgcttcagga cgccgtcggc atcgtgacca ccagttggga tgatttcatt
    25441 tttaaatgcg cccgactgcg tggcggccca ggcgcgggcg tgtgaccgcg cggcaaaggc
    25501 atcctgcatt tcacggctga taccgtgcat acgcgccagc atttctgccg ttaagcccat
    25561 catgcccgcc gctttggcga cattgcggct caggccaggg tgaaaatcga cgccgtgact
    25621 catcggcaca tggcccatat gctccacgcc gccaaccaga catgcctgcg catcgccggt
    25681 catgatcatt cgtgctgcgt catgcagtgc ctgcatggat gaaccacaca agcgattgac
    25741 agtaaccgcc gggacagagt gcggcacttc tgccagcagc gccgcgttac gggcgatatt
    25801 aaaaccctgc tccagcgtct gctgcacaca cccccagtaa atatcgtcga gggccgccgc
    25861 ttccagcgcc gggttacgcg ccagcaggct acgcattaaa tgagcggaga gatcttctgc
    25921 acgcacgtta cgaaaagcac cgcccttcga acggcccatc ggggtgcgaa ttgcatcgac
    25981 aatgacaacc tgttccattg tgactcctta agccgttttc aggtcgccaa ccggacgggc
    26041 tggctcaacc gggggatagt acggttcgtt atgacgcgct ttattacgca gaccttccgg
    26101 cacttcgtac agcgggccga ggtgctgata ttgctgtgcc atatcgaggt attttgcgct
    26161 gccgagggtg tccagccagc ggaacgcgcc gccgtggaac ggagggaagc ccaggccgta
    26221 gaccagcgcc atatccgctt ctgccggagt ggcgataatg ccttcttcca gacagcgcac
    26281 cacttcgttg accatcggga tcatcatgcg ggcgataatc tcttcttcgc tgaaatcgcg
    26341 cttcggctgg ctcacttctg ccagcaggtc gtcaacagcg gcgtcttctt ctttcttcgg
    26401 cttacctttg ctgtcttctt tataatgcca gaaaccgagg ccgttcttct gaccaaagcg
    26461 gttggcatca aacagcgcgt cgatggcatc gcgataatct ttctgcatcc gctgtgggaa
    26521 gcccgctgcc atgactgcct gggcgtgatg cgcggtgtca atgcccacaa cgtccagcag
    26581 atatgccgga cccataggcc agccaaactg tttttccatc actttgtcga tcttgcggaa
    26641 atccgcgccg tcgcgcagca actggctgaa accggcgaaa tacgggaaca gcacgcggtt
    26701 gacgaagaag ccggggcaat cgttaaccac aatcggcgtt ttacccatct tgctcgccca
    26761 ggcgacgact ttcgcaatag tttcgtcgga gcttttctcg ccgcgaataa tttccaccaa
    26821 cggcattcgg tggaccgggt taaagaagtg catcccgcag aagttttccg gacgttccag
    26881 cgcgttggcc agttcgctga taggaatggt tgaagtgtta gacgccagca cggtatccgg
    26941 gcgtactttc tgctcggttt ctgccagcac ggcttttttc actttcgggt tttcaacaat
    27001 cgcttctacc acaacatcca cgcgctcaaa tccggcgtag tcgagcgttg ggtggatcgt
    27061 ggagatcacg ccagccagtt tcagaccatc gatcttgccg cgctcaagct gcttgttcag
    27121 tagtttcgcg gcttcagtca tgccgagggc taacgacttg tcgttaatat ctttcataat
    27181 caccggcacg cctttccacg cagactggta agcgatgccg ccacccataa tgcctgcgcc
    27241 cagcacggcg gcctgtttcg gggtttcaac gtctttggtg agtttcttcg ctttgccttt
    27301 tacatattga tcgttaagga aaatgccgac cagtgcgcga gcttcgttgg tatgcgccag
    27361 cgggacaaag cttttgtttt ctaggtttaa ggcttcttca cgaccaaaac gagccgcagc
    27421 ttcaatggtt tttactgcgg tgatgggggc cgggtaatgt tttcctgccg tttgtgcgac
    27481 catcccttta gcgatggtga agctcatggt ggcttcaatc ttgctcagtt ttagcggttc
    27541 cagcttcggc tgacgtttcg ctttccagtc gaggtcgccg ttaatggcct ggcgtaaaat
    27601 cgccatcgcc ccttcaatca gtttttctgc tttgactacg ccatccacca gaccgatttt
    27661 cagcgcctga tccgcgccga catctttacc ggcggcaatg atttccagcg cactgtcagc
    27721 gcccagcata cgtggcatac gtacagaacc gccaaagccc ggcatgatgc ccagtttggt
    27781 ttccggcaga ccgatgcgca gatccggcgt cgccagacgg taatcggtcg ccagcacgca
    27841 ttcgcagcca ccgcccagcg catagccatt gacggcagca atggtcggca ccggcagatc
    27901 ttccaggcga ttaaacacgc tattggcaaa gtgcagccac tgacttaact gttcttcagg
    27961 aacgaggaac agggacaaaa attcggtgat atcagcaccg acgataaagg ctgctttgtt
    28021 cgaacgcagc agcagccctt ttagatctga ttgctgttcc agcacgccga tggcctcgcc
    28081 gaggctggcg acggtcgcag tgtcgagttt attaactgaa cctggggcat caaataccag
    28141 ttcggcaatg ccatcttcca gccagtcaag gtacagggtg tcgcctttgt aaagcatgtc
    28201 agtctcctga atccgcaagg tgatctggtc gtaccagatg catcgaagtg tgtattttgt
    28261 gttaaaaata tgcaaataaa agattaaaga aatgccgatc tgatcacgct cggcagaaat
    28321 cacgctctgg atgaacgatg tgctaagatg cggagactta aggtcaaaaa aacagaaggg
    28381 taaaaaatgg aatcactggc ctcgctctat aaaaatcata tagctacatt gcaggaacga
    28441 actcgcgatg cgctgacgcg cttcaagctg gatgcgttac ttattcactc cggcgaactg
    28501 ttcaacgttt ttctcgacga tcatccctat ccgtttaaag tgaacccgca attcaaagcg
    28561 tgggtgccgg taactcaggt gccaaactgc tggctgctgg tggatggcgt gaataagccg
    28621 aaactgtggt tctatctgcc ggttgattac tggcacaacg tcgaaccgct gccgacctcc
    28681 ttctggactg aagatgtaga agtgatcgcg ctgccgaaag ccgatggcat tggtagtctg
    28741 ttgcctgctg cgcgcggcaa tatcggttat atcggtccgg tgccggaacg tgcgctgcaa
    28801 ctgggtattg aggccagcaa tatcaacccg aaaggggtta tcgactacct gcattactac
    28861 cgctccttca aaaccgagta cgagctggcc tgtatgcgtg aagcgcagaa aatggcggtc
    28921 aacggtcatc gcgcggcaga agaagcgttc cgttctggca tgagcgagtt cgatatcaat
    28981 attgcctatc tgactgcgac cggtcatcgt gataccgacg taccttacag caacattgtg
    29041 gcactcaacg aacacgctgc ggtgctgcat tacaccaaac tggatcatca ggcgtcggaa
    29101 gagatgcgca gcttcctgct ggatgccggg gccgaatata acggctatgc cgctgacctg
    29161 acccgtacct ggtcggcaaa aagtgacaac gattacgcac agctggtgaa agacgtaaat
    29221 gatgaacaac tggcgctgat cgcgaccatg aaagctggcg ttagctatgt ggattaccac
    29281 atccagttcc atcagcgcat cgccaaattg ctgcgtaaac atcaaatcat caccgatatg
    29341 agtgaagagg cgatggtcga aaacgatctt accgggccgt ttatgccgca tggtatcggc
    29401 catccgctgg gcctgcaggt gcatgacgtc gccggtttta tgcaggatga tagcggtacg
    29461 cacctcgcgg caccggcaaa atatccgtac ctgcgctgca cccgtattct ccagccgggc
    29521 atggtgttaa ccatcgaacc gggtatctac ttcattgaat cgctgctggc accgtggcgt
    29581 gaagggcagt tcagcaagca cttcaactgg cagaaaattg aagcactgaa accgttcggc
    29641 ggcattcgta tcgaagacaa cgtggtgatc cacgaaaata acgtggaaaa catgacccgg
    29701 gatctgaaac tggcgtgatg gaaagctggt taattcctgc ggcaccggtc acggtcgttg
    29761 aagagatcaa aaaaagccgc ttcattacgc tgttggcgca taccgatggc gttgaggcgg
    29821 cgaaagcgtt tgttgaatcg gtgcgggcag aacaccccga tgcccgccac cattgcgtgg
    29881 cgtgggtggc gggtgcgccg gatgattctc aacagctggg tttctctgac gacggggagc
    29941 cggcgggaac ggcaggtaaa ccgatgctcg cccagctaat gggcagcggc gttggggaaa
    30001 ttaccgctgt ggtggtgcgc tactacggcg gcatattgct tggcaccggt gggttagtga
    30061 aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct aacgacccaa cgcaagacgc
    30121 cattaaccga atatactttg caatgtgaat atagtcagtt aaccggcatt gaagcgttgc
    30181 tggggcagtg tgacggcaaa attatcaaca gtgattatca ggcattcgtt ctgctgcggg
    30241 tggcgcttcc ggcggcgaaa gtggctgaat tttccgcgaa gctggcggat tttagccgtg
    30301 gttcattgca attgttagcg attgaagaat aatccccact tcgttttgca gaactaagga
    30361 agcggcagag atgcattttc gcgccattac ccgaatcgtt ggactactgg tcatcttatt
    30421 ttcagggacc atgattatcc ctgggctggt agcactcatc taccgggatg gagcgggccg
    30481 cgcttttacc cagacctttt ttgtcgccct cgccattggc tctatgctgt ggtggccgaa
    30541 ccgcaaagag aagggcgaac tgaaatcccg tgaggggttt ctgatagtgg tgctgttctg
    30601 gaccgtgctg ggtagtgtcg gtgcgctccc ttttatcttc tcggaaagcc cgaacctcac
    30661 gattaccgat gcgttttttg aatctttctc tggcctgacc accaccgggg ccactacgct
    30721 ggtggggctg gattcgctcc ctcatgccat cctcttttat cgccagatgc tgcaatggtt
    30781 tggcgggatg gggatcatcg tgttagcggt tgcgatactg cctatcctcg gcgtgggtgg
    30841 gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa gataacaaaa tgcgcccgcg
    30901 aattgcggaa acggcgaaaa ccctgtggtt gatttatgtc ttgctgaccg tcgcctgtgc
    30961 gctggcgttg tggtttgccg gaatggatgc ctttgatgcc atcggccata gctttgcgac
    31021 tatcgctatt ggcggcttct cgacacatga tgccagtatc ggttatttcg atagcccgac
    31081 tattaacact atcattgcta tcttcctgct gatctccggc tgtaactacg gtctgcactt
    31141 ttcactgtta agtgggcgta gtctgaaggt ttattggcgc gatccggaat ttcgcatgtt
    31201 tatcggcgta cagtttacgc tggtggttat ttgtaccctc gtactgtggt ttcataatgt
    31261 ctacagttcg gcgctgatga caattaacca ggcgtttttc caggtggtgt cgatggcgac
    31321 aaccgccggg tttacgactg acagcattgc ccgctggccg ctctttttgc cggtactgct
    31381 tttatgttca gcttttatcg gcggttgtgc cgggtcaacg ggcggtggcc tgaaagtgat
    31441 ccgcatcctg ctgctgttta agcaggggaa ccgtgaactg aaacgactgg tgcatccgaa
    31501 cgccgtctat agcattaagc tggggaatcg cgcactgccg gaacgtatcc tcgaagccgt
    31561 ttggggattt ttctccgcct atgcattggt gtttattgtc agtatgctgg cgattatcgc
    31621 cacgggcgtg gatgactttt ctgcctttgc ctcggttgtt gcgacattga ataacctggg
    31681 gccagggctt ggcgtggttg ctgataactt taccagtatg aacccggtgg ctaaatggat
    31741 cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt acattgctgg tgctctttac
    31801 cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa cattaattct tttctcaaca
    31861 agggacggac aaacgcgcga gattgcctcc tacttggctt cggaactgaa agaactgggg
    31921 atccaggcgg atgtcgccaa tgtgcaccgc attgaagaac cacagtggga aaactacgac
    31981 cgtgtggtca ttggtgcttc tattcgctat ggtcactacc actctgcgtt ccaggaattt
    32041 gtcaaaaagc atgcgacgcg gctgaattcg atgccgagcg cgttttactc cgtaaacctg
    32101 gtggcgcgta aaccggagaa gcgcactcca cagaccaaca gctacgcgcg gaagtttctg
    32161 atgaactcgc aatggcgtcc cgatcgctgc gcggtcattg ccggggcgct gcgttaccca
    32221 cgttatcgct ggtacgaccg ttttatgatc aagctgatta tgaagatgtc aggcggtgaa
    32281 acggatacgc gcaaagaagt tgtctatacc gattgggagc aggtggcgaa tttcgcccga
    32341 gaaatcgccc atttaaccga caaaccgacg ctgaaataag cataaagaat aaaaaatgcg
    32401 cggtcagaaa attattttaa atttcccctt gtcaggccgg aataactccc tataatgcgc
    32461 caccactgac acggaacaac ggcaaacacg ccgccgggtc agcgggattc tcctgagaat
    32521 tccg
//
LOCUS       DAZFBR010000048        32341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFBR010000048 DAZFBR010000000
VERSION     DAZFBR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.048"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(155..1915)
                     /gene="ycaO"
                     /locus_tag="ACHJZ2_002437"
     CDS             complement(155..1915)
                     /gene="ycaO"
                     /locus_tag="ACHJZ2_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO4568526.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            2045..2737
                     /locus_tag="ACHJZ2_002438"
     CDS             2045..2737
                     /locus_tag="ACHJZ2_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO4568527.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2936..4024
                     /gene="serC"
                     /locus_tag="ACHJZ2_002439"
     CDS             2936..4024
                     /gene="serC"
                     /locus_tag="ACHJZ2_002439"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO4568528.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4095..5378
                     /gene="aroA"
                     /locus_tag="ACHJZ2_002440"
     CDS             4095..5378
                     /gene="aroA"
                     /locus_tag="ACHJZ2_002440"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO4568529.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5547..6311
                     /gene="ycaL"
                     /locus_tag="ACHJZ2_002441"
     CDS             5547..6311
                     /gene="ycaL"
                     /locus_tag="ACHJZ2_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO4568530.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6484..7167
                     /gene="cmk"
                     /locus_tag="ACHJZ2_002442"
     CDS             6484..7167
                     /gene="cmk"
                     /locus_tag="ACHJZ2_002442"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO4568531.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7278..8951
                     /gene="rpsA"
                     /locus_tag="ACHJZ2_002443"
     CDS             7278..8951
                     /gene="rpsA"
                     /locus_tag="ACHJZ2_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO4568532.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9111..9395
                     /gene="ihfB"
                     /locus_tag="ACHJZ2_002444"
     CDS             9111..9395
                     /gene="ihfB"
                     /locus_tag="ACHJZ2_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO4568533.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9602..11866
                     /locus_tag="ACHJZ2_002445"
     CDS             9602..11866
                     /locus_tag="ACHJZ2_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO4568534.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GQRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVVILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILDQGLWFLADRSLALLFLGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGRNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11903..13651
                     /gene="msbA"
                     /locus_tag="ACHJZ2_002446"
     CDS             11903..13651
                     /gene="msbA"
                     /locus_tag="ACHJZ2_002446"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO4568535.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13648..14634
                     /gene="lpxK"
                     /locus_tag="ACHJZ2_002447"
     CDS             13648..14634
                     /gene="lpxK"
                     /locus_tag="ACHJZ2_002447"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO4568536.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14671..15904
                     /gene="ycaQ"
                     /locus_tag="ACHJZ2_002448"
                     /pseudo
     CDS             14671..15904
                     /gene="ycaQ"
                     /locus_tag="ACHJZ2_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
     gene            15956..16138
                     /gene="ycaR"
                     /locus_tag="ACHJZ2_002449"
     CDS             15956..16138
                     /gene="ycaR"
                     /locus_tag="ACHJZ2_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO4568537.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16135..16881
                     /gene="kdsB"
                     /locus_tag="ACHJZ2_002450"
     CDS             16135..16881
                     /gene="kdsB"
                     /locus_tag="ACHJZ2_002450"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO4568538.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            17035..17928
                     /locus_tag="ACHJZ2_002451"
     CDS             17035..17928
                     /locus_tag="ACHJZ2_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO4568539.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17905..18684)
                     /gene="elyC"
                     /locus_tag="ACHJZ2_002452"
     CDS             complement(17905..18684)
                     /gene="elyC"
                     /locus_tag="ACHJZ2_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO4568540.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18820..19605
                     /gene="cmoM"
                     /locus_tag="ACHJZ2_002453"
     CDS             18820..19605
                     /gene="cmoM"
                     /locus_tag="ACHJZ2_002453"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO4568541.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19602..20924
                     /gene="mukF"
                     /locus_tag="ACHJZ2_002454"
     CDS             19602..20924
                     /gene="mukF"
                     /locus_tag="ACHJZ2_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO4568542.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20905..21609
                     /gene="mukE"
                     /locus_tag="ACHJZ2_002455"
     CDS             20905..21609
                     /gene="mukE"
                     /locus_tag="ACHJZ2_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO4568543.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21609..26069
                     /gene="mukB"
                     /locus_tag="ACHJZ2_002456"
     CDS             21609..26069
                     /gene="mukB"
                     /locus_tag="ACHJZ2_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO4568544.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26330..28177
                     /gene="ldtD"
                     /locus_tag="ACHJZ2_002457"
     CDS             26330..28177
                     /gene="ldtD"
                     /locus_tag="ACHJZ2_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4568545.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28358..28906
                     /locus_tag="ACHJZ2_002458"
     CDS             28358..28906
                     /locus_tag="ACHJZ2_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO4568546.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28933..29580
                     /gene="gloC"
                     /locus_tag="ACHJZ2_002459"
     CDS             28933..29580
                     /gene="gloC"
                     /locus_tag="ACHJZ2_002459"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO4568547.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29631..30821)
                     /gene="aspC"
                     /locus_tag="ACHJZ2_002460"
     CDS             complement(29631..30821)
                     /gene="aspC"
                     /locus_tag="ACHJZ2_002460"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO4568548.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31006..32094)
                     /gene="ompF"
                     /locus_tag="ACHJZ2_002461"
     CDS             complement(31006..32094)
                     /gene="ompF"
                     /locus_tag="ACHJZ2_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO4568549.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7899 a         7860 c         8668 g         7914 t
ORIGIN      
        1 cgatcgtatt tattaaatac aaattaccga tatttaacct tataattaca attattttat
       61 taatgcaaat atatgtaaag cggggcatta aaaaaacgcc ccgtaatata actcagacga
      121 atcattaagc ctacattacg taggctattt gattttattt tgcccagaat gctgctttgg
      181 cgcgctgcag cttttcgtag gccttcaaca gcgactgatg tgcagcaaac gcgtgcagat
      241 cgctatctac tggttgcaag ccataaaacg ccgcttcgcc gctcattgcc gcactggcgg
      301 cttccacggc atctgcgccg tacatgcgaa caaacgcatt cagatattgc agcggctggc
      361 gatcttcttc ctgcgccagt aataacaacg tttgcaggca gcgataatag ttggcgcgtt
      421 ccgggctgaa taccgatgag ttaaactcca tcgtccattc ggtccagacc agagcctgtt
      481 ccagatcgcc accagccagc gccagcatgg cttttaattc accaatacgc agggtgtacc
      541 aaccgttatc cgacccggtc gccagaccca acagctcacg cacgcgggta aagtcatcaa
      601 aaccttcttc atccagttgc tcgatgaggt tcaggtaatc ttctttttcc cactcgctgc
      661 ctggtagcga aagaatcgtt tcacgtaaat ggctgcccat actgttattc gcgagccaca
      721 gatcttcagc cggataaata tcggacatgc caggcacgat aatacggcaa gcataaacgc
      781 ccagatgctc gtaatcggca atataaactt ctttatcttc tttgttgaag atagccatca
      841 gcgtagcaaa ctcttcttcc gtggtgccgg agaaattcca gtccacaaac ggataatcgg
      901 catcctgctt gaacaggtcc caggagatta aaccgctgga atcgataaag tgcgtttcga
      961 ggttggtatg ttcagcgact tcttcatcat cgaaggttgg cggagtaaac acatccaaat
     1021 ctttcaggcc acgaccttgc agcagctcgg tcacggtacg ttccagtgct acgccaaaat
     1081 caggatgcgc accgaaagag gcaaagcagg tgccgttagc aggattaaac agtaccacgc
     1141 aaatcaccgg atactggccg ccaagggaac catcatatgc gaagatcggg aaaccttctg
     1201 cttccagtgt ttcgatcgct tcaaccaccg ctgggtaacg cgccagcacg tctgccggga
     1261 tttccggcaa gctgatgctt tcagcaataa tgcggttttt cacgtagcgt tcgaaaactt
     1321 cggacaaccc ctgaacgcgt gcttcgttac gggtattgcc agcagacata ccgttagaaa
     1381 cgtacaggtt accaatgata ttcatcggaa tataaacggt ctgattatca gactgacgtg
     1441 taaacggcag accgcaaata ccacgatctt cgttaccgga ttgtaggtca atcagcatac
     1501 tgccggtcaa ttcattctcc ggatcgtaaa atgcgcgcag acgttcatcg agcagccctt
     1561 ctggcacatc gtcattttcg gtcagtggga accatttttc gttgggataa tgcacaaatg
     1621 gaccgttggc gatggtttcg cccagccaga aatcagcaaa aaagtagttg gttgagagac
     1681 gctcgaaata ttcaccgagt gcagaagcca gcgccgcttt cttggttgcg cctttaccgt
     1741 tggtaaaaca cagtgcgcac tctttgtcgc gaatatgtac agaccagacg ttaggcacgg
     1801 gattcagcca ggaggcctct tcaatctgaa agccgaggtc tgaaagtttt tgctggaagc
     1861 gagcgatgga atcttccagt gcggcatctt tgccggggat aaatgtttgc gtcatgaaaa
     1921 tcactttagt cgtacggaaa gcgcgcaata atacgggttt tatctcaaag gcgctatcac
     1981 cgccgccatg ccgatgaact gttgactatg cttttagcgg ataaaccacg ttaagagcat
     2041 aaaaatgaag gcgttcgatc tccaccgtat ggcatttgat aaagtgcctt ttgatttcct
     2101 tggcgaagtt gcactacgta gtctttatac ctttgtactg gtctttttgt tcctcaaaat
     2161 gaccggaaga cgcggtgtgc ggcagatgtc gctgtttgaa gttttaatca ttctgacgct
     2221 gggatcagcg gcgggagatg tggcgtttta tgatgatgtg ccgatggtcc cggtacttat
     2281 cgtctttatt actctggcgt tgttataccg cctggtaatg tggttgatgg cgcacagtga
     2341 aaaactggaa gatcttctgg aaggcaagcc agttgtcatt attgaagatg gcgagctggc
     2401 ctggtcgaaa ctcaataact ccaacatgac ggaatttgag ttctttatgg agctacgatt
     2461 gcgtggcgtg gagcagctgg ggcaggtacg tctggcgatt ctcgaaacca acgggcaaat
     2521 cagtgtctat ttctttgaag atgacaaggt gaaatcgggt ttacttattt tacccagtga
     2581 ttgtacgcag cgttacaaag tggtgccgga gtcggcggac tatgcctgta ttcgttgtag
     2641 tgaaatcatt catatgaaag cgggggaaaa acaattatgt ccgcgctgtg caaatccaga
     2701 atggacgaag gcaagtcggg caaaacgggt gacctgacag taaaaacatc ggctttttgc
     2761 taataatccg agagattctt ttgtgtgatg caagccacat ttttgccctc aacggtttta
     2821 ctcattgcga tgtgtgtcac tgaatgataa aaccgatagc cacaggaata atgtattacc
     2881 tgtggtcgca atcgattgac cgcgggttaa tagcaacgca acgtggtgag gggaaatggc
     2941 tcaaatcttc aattttagtt ctggtccggc aatgctaccg gtagaggtgc ttaaacaggc
     3001 tcaacaggaa ctgcgcgact ggaacggtct tggtacgtcg gtgatggaag tgagtcaccg
     3061 tggcaaagag ttcattcagg ttgcagagga agccgagaag gattttcgcg atcttcttaa
     3121 tgtcccctcc aactacaagg tattattctg ccatggcggt ggtcgcggtc agtttgctgc
     3181 ggtaccgctg aatattctcg gtgataaaac caccgcagat tatgttgatg ccggttactg
     3241 ggcggcaagt gccattaaag aagcgaaaaa atactgcacg cctaatgtct ttgacgccaa
     3301 agtgactgtt gatggtctgc gcgcggttaa gccaatgcgt gaatggcaac tctctgataa
     3361 tgctgcttat atgcattact gcccgaatga aaccatcgac ggtatcgcca tcgacgaaac
     3421 gccagacttc ggcaaagatg tggtggtcgc cgccgacttc tcttcaacca ttctttcccg
     3481 tccgattgac gtcagccgtt atggtgtaat ttacgctggc gcgcagaaaa atatcggccc
     3541 ggcaggcctg acaatcgtca tcgtccgtga agatttgctg ggcaaagcga atgtcgcgtg
     3601 tccgtcgatt ctggattatt ccatcctcaa cgataacggc tccatgttta acacgccgcc
     3661 gacatttgcc tggtatctat ctggtctggt ctttaaatgg ctgaaagcga acggcggtgt
     3721 agctgaaatg gataaaatca atcagcaaaa agcagaactg ctatatgggg tgattgataa
     3781 cagcgatttc taccgcaatg acgtggcgaa agctaaccgt tcgcggatga acgtgccgtt
     3841 ccagttggcg gacagtgcgc ttgacaaatt gttccttgaa gagtcttttg ctgcaggcct
     3901 tcatgcactg aaaggtcacc gtgtggtcgg cggaatgcgc gcgtctattt ataatgccat
     3961 gccgctggaa ggcgttaaag cgctgacaga cttcatggtt gagttcgaac gccgtcacgg
     4021 ttaatgccga aattttgctt aatccccaca gccagcctgt ggggttttta tttctgttgt
     4081 agagagttga gttcatggaa tccctgacgt tacaacccat cgctcgtgtc gatggcacta
     4141 ttaatctgcc cggttccaag agcgtttcta accgcgcttt attgctggcg gcattagcac
     4201 acggcaaaac agtattaacc aatctgctgg atagcgatga cgtgcgccat atgctgaatg
     4261 cattaacagc gttaggggta agctatacgc tttcagccga tcgtacacgt tgcgaaatta
     4321 tcggtaacgg cggtccatta cacgcagaag gtgccctgga gttgttcctc ggtaacgccg
     4381 gaacggcaat gcgtccgctg gcggcagctc tttgtctggg tagcaatgat attgtgctga
     4441 ccggtgagcc gcgtatgaaa gagcgcccga ttggtcatct ggtggatgcg ctgcgcctgg
     4501 gcggggcgaa gatcacttac ctggaacaag aaaattatcc gccgttgcgt ttacagggcg
     4561 gctttactgg cggcaacgtt gacgttgatg gctccgtttc cagccaattc ctcaccgcac
     4621 tgttaatgac tgcgcctctt gcgccggaag atacggtgat tcgtattaaa ggcgatctgg
     4681 tttctaaacc ttatatcgac atcacactca atctgatgaa gacgtttggt gttgaaattg
     4741 aaaatcagca ctatcaacaa tttgtcgtaa aaggcgggca gtcttatcag tctccgggta
     4801 cttatttggt cgaaggcgat gcatcttcgg cttcttactt tctggcagca gcagcaatca
     4861 aaggcggcac tgtaaaagtg accggtattg gacgtaacag tatgcagggt gatattcgct
     4921 ttgctgatgt gctggaaaaa atgggcgcga ccatttgctg gggcgatgat tatatttcct
     4981 gcacgcgtgg tgaactgaac gctattgata tggatatgaa ccatattcct gatgcggcga
     5041 tgaccattgc tacggcggcg ttatttgcaa aaggcaccac cacgctgcgc aatatctata
     5101 actggcgtgt taaagaaacc gatcgcctgt ttgcgatggc aacagaactg cgtaaagtcg
     5161 gtgcggaagt agaagagggg cacgattaca ttcgtatcac tccaccggaa aaactgaatt
     5221 ttgccgagat cgcgacatac aatgatcacc ggatggcgat gtgtttctcg ctggtggcgt
     5281 tgtcagatac accagtgacg attcttgatc ccaaatgtac ggccaaaaca tttccggatt
     5341 atttcgagca gctggcgcgg attagccagg cagcctgaat gaataacgtc caataaatag
     5401 ccaaaacttt ctttatcaaa acgtcggcac attgccggcg ttttttttcg gaccttgtga
     5461 gtcattttga ttaatggtag cgtcgcttgt caatgtaagt tgttgataca taatgtttat
     5521 atatgattaa tcaacggatg attcacatga agaatactaa attactgctg gcgattgtga
     5581 cctctgcagc attactgacg gggtgtcaaa atacccacgg tattgatacc aatatggcta
     5641 tcagctccgg tttaaatgcc tataaagcag caacgttaag cgatgccgat gcaaaagcga
     5701 ttgccaatca gggctgtgcc gaaatggaca gcggcaatca agtcgcaagt aaatccagca
     5761 agtacggtaa acgtctggca aaaatcgcca aagcattggg taacaatatt aatggcacgc
     5821 cggtcaacta taaggtttat atgaccagcg acgtcaacgc atgggcgatg gcgaacggct
     5881 gtgttcgtgt ttacagtggc ctgatggaca tgatgaacga taacgaaatt gaaggcgttc
     5941 tgggccatga actgggccac gtcgcgttgg gtcactcgct ggctgaaatg aaagcttctt
     6001 atgcgatcgt tgccgcacgc gatgccattt cagctaccag cggtgtggct tcccagcttt
     6061 cccgctcaca attgggtgat atcgcagaag gcgctatcaa tgcgaaatac tcccgcgata
     6121 aagagtccga agcagatgat ttctcctttg atctgttgaa gaaacgtggc atcagcaccc
     6181 aggggctggt tggcagcttt gaaaaactgg ctagcctgga tggcggtcgc acccagtcca
     6241 tgtttgactc tcacccacca tcaacagagc gtgcgcaaca catccgtgat cgtatcgcct
     6301 ctggtaagta aatcattgtc atctttcggg ctggtcttct gccagcccgc tataattgcg
     6361 caataaatcc ccatctgaat acagacaaaa ctggtttttg cacacaacgt taacgatttg
     6421 tggcgtcggc gcgtataatg cgcgcggtta tgttaacggt acgcctgttt taaggagata
     6481 aagatgacgg caattgcccc ggttattacc attgatggcc caagcggtgc agggaaaggc
     6541 accttgtgta aggctatggc ggaagcgttg caatggcatc tgctggattc gggtgcaatt
     6601 tatcgcgtac tggcactagc ggcattacat caccatgttg atgttgcatc ggaagatgcg
     6661 ctggtgccgc tggcatccca tctggatgta cgttttgtgt caaccaatgg caatctggaa
     6721 gtgatcctcg aaggggaaga tgtcagcggc gaaattcgta ctcaggaagt ggcgaatgca
     6781 gcttcacaag tcgcggcatt cccacgcgtt cgtgaagcat tattgcgtcg ccaacgcgcg
     6841 tttcgcgaat taccaggcct gattgccgat ggccgcgaca tgggaacggt ggtattccct
     6901 gatgcgccag tgaaaatttt ccttgacgcc tcctcggaag aacgtgcgca tcgccgcatg
     6961 ctacagttgc aggagaaggg ctttagtgtt aactttgagc gccttttggc cgagatcaaa
     7021 gaacgcgacg accgcgatcg taaccgagcg gtagcgccac tggttccggc agccgatgct
     7081 ttagtgttgg attccaccac cttaagcatt gagcaagtga ttgaaaaagc gctacaatac
     7141 gcgcgccaga aattggctct cgcataagcg accgaatttg cagtaccccc gttgcaatgg
     7201 aatgacagcg ggtatgttaa acaaccccat ccggcatgga gccaggtgga cgttaaatat
     7261 aaacctgaag attaaacatg actgaatctt ttgctcaact ctttgaagag tccttaaaag
     7321 aaatcgaaac ccgcccgggt tctatcgttc gtggcgttgt tgttgctatc gacaaagacg
     7381 tagtactggt tgacgctggt ctgaaatctg agtccgccat cccggctgag cagttcaaaa
     7441 acgcccaggg cgagctggaa atccaggtag gtgacgaagt tgacgttgct ctggacgcag
     7501 tagaagacgg cttcggtgaa actctgctgt cccgtgagaa agctaaacgt cacgaagcct
     7561 ggatcacgct ggaaaaagct tacgaagatg ctgaaactgt taccggtgtt atcaacggca
     7621 aagttaaggg cggcttcact gttgagctga acggtattcg tgcgttcctg ccaggttctc
     7681 tggtagacgt tcgtccggtg cgtgacactc tgcacctgga aggcaaagag cttgaattca
     7741 aagtaatcaa gctggatcag aagcgcaaca acgttgttgt ttctcgtcgt gccgttatcg
     7801 aatccgaaaa cagcgcagag cgcgatcagc tgctggaaaa cctgcaggaa ggcatggaag
     7861 ttaaaggtat cgttaagaac ctcactgact acggtgcatt cgttgatctg ggcggcgttg
     7921 acggcctgct gcacatcact gacatggcct ggaaacgcgt taagcatccg agcgaaatcg
     7981 tcaacgtggg cgacgaaatc actgttaaag tgctgaagtt cgaccgcgaa cgtacccgtg
     8041 tatccctggg cctgaaacag ctgggcgaag atccgtgggt agctatcgct aaacgttatc
     8101 cggaaggtac caaactgact ggtcgcgtga ccaacctgac cgactacggc tgcttcgttg
     8161 aaatcgaaga aggcgttgaa ggcctggtac acgtttccga aatggattgg accaacaaaa
     8221 acatccaccc gtccaaagtt gttaacgttg gcgatgtagt ggaagttatg gttctggata
     8281 tcgacgaaga acgtcgtcgt atctccctgg gtctgaaaca gtgcaaagct aacccgtggc
     8341 agcagttcgc ggaaacccac aacaagggcg accgtgttga aggtaaaatc aagtctatca
     8401 ctgacttcgg tatcttcatc ggcctggacg gcggcatcga cggcctggtt cacctgtctg
     8461 acatctcctg gaacgttgca ggcgaagaag cagttcgtga atacaaaaaa ggcgacgaaa
     8521 tcgctgcagt tgttctgcag gttgacgcag aacgtgaacg tatctccctg ggcgttaaac
     8581 agctcgcaga agatccgttc aacaactggg ttgctctgaa caagaaaggc gctatcgtaa
     8641 ccggtaaagt aactgcagtt gacgctaaag gcgcaaccgt agaactggct gacggcgttg
     8701 aaggttacct gcgtgcttct gaagcatccc gtgaccgcgt tgaagacgct accctggttc
     8761 tgagcgttgg cgacgaagtt gaagctaaat tcaccggcgt tgatcgtaaa aaccgtgcaa
     8821 tcagcctgtc tgttcgtgcg aaagacgaag ctgacgagaa agatgcaatc gcaactgtta
     8881 acaaacagga agatgcaaac ttctccaaca acgcaatggc tgaagctttc aaagcagcta
     8941 aaggcgagta attctctgac tcttcgggat ttttattctg aagtttgttg agtttacttg
     9001 acagattgca ggtttcgtcc tgtaatcaag cactaagggc ggctacggcc gcccttaatc
     9061 aatgcagcaa cagcagccgc ttaatttgcc tttaaggaac cggaggaatc atgaccaagt
     9121 cagaattgat agaaagactt gccacccagc aatcgcacat tcccgccaag acggttgaag
     9181 atgcagtaaa agagatgctg gagcatatgg cctcgactct tgcgcagggc gagcgtattg
     9241 aaatccgcgg tttcggcagt ttctctttgc actaccgcgc accacgtacc ggacgtaatc
     9301 cgaagactgg cgataaagta gaactggaag gaaaatacgt tcctcacttt aaacctggta
     9361 aagaactgcg cgatcgcgcc aatatttacg gttaagtttt tactcaaact tgaacgagag
     9421 aaaagcacct gtcgggtgct tttttcattt atctaatctg gaactggaag ctgcctcgca
     9481 gagttttgag cagttttcac cctttcgtta aattcttctg aatgtgcctc ggggaacgca
     9541 aaattcccac cagacaaccg ctcaacaaag ttgcacactt tccataaaca gggaggggtg
     9601 catgaaaata acgacagtcg gcgtatgcat aatttgcgga atttttccgt tgctgatttt
     9661 gccccaattg cctgggacat taacccttgc gtttctgact ctcttcgcct gcgtactggc
     9721 atttatccct gttaaaaccg tccgttatat cgcgctgacg ttgctgtttt tcgtttgggg
     9781 catattatca gcaaagcaaa ttttgttggc aggagaaacc ttaactggcg cgacgcagga
     9841 tgcaattgtt gagatcactg ctactgacgg catgaccact cattacggtc aaattactta
     9901 tctacaaggt caacgtatat tccctgcgcc aggccttgtg ctgtatggcg aatatcttcc
     9961 gcaagcggtt tgtgccggac aactgtggtc aatgaaactc aaagttcgtg ccgttcatgg
    10021 tcaacttaat gatggcggct ttgatagcca gcgttatgcc attgcccagc atcagccgct
    10081 caccggccgc tttctgcagg caagtgttat tgaaccgaat tgtagcctgc gtgcacagta
    10141 tctggcgtca ctacaaacaa cgctgcaacc ctatccgtgg aatgcggtta ttcttggttt
    10201 aggtatgggg gaacggttat ccgtccctaa agaaatcaaa aatatcatgc gtgatactgg
    10261 aacggcgcat ttaatggcga tatcgggatt gcacatcgct tttgcggcgt tgctggctgc
    10321 cggactcatt cgcagtggac aaatttttct gcctgggcgc tggatccact ggcaaatgcc
    10381 attaattggc ggaatctgct gtgctgcttt ttatgcctgg ctgacgggaa tgcaacctcc
    10441 tgcattgcgt accgttgtgg cgcttgctac gtggggtatg cttaagttaa gtgggcgaca
    10501 gtggagtggc tgggatgtat ggatatgttg tctggcggca attttgctga tggatcctgt
    10561 tgtcattctc tcgcaaagtt tatggctctc tgccgctgcg gtcgcggcat tgatattttg
    10621 gtatcagtgg tttccctgtc ctgagtggca actgccgccg gtattgcgtg cagttgtttc
    10681 cctcatccat ctgcaactgg gaatcacact tctgcttatg cccgtgcaaa tcgtcatttt
    10741 tcatggcatt agtctgacct cgtttattgc aaatctatta gcaattccct tggtgacatt
    10801 tatcacggtt ccgttgatcc tcgccgcgat ggttgtgcat ttaagcgggc cgttaatcct
    10861 ggatcaaggg ttatggtttc ttgccgaccg gtctttggct ttacttttct tggggttaaa
    10921 gagtttgcca gaagggtgga tcaacattgc tgaacgttgg caatggctat cattttcccc
    10981 atggttctta ctggtggtat ggcgattaaa cgcctggcga acgttgccag caatgtgtgt
    11041 ggctgtaggc ttgctgatgt gctggccgct gtggcaaaaa cctcgacctg acgagtggca
    11101 agtgtacatg cttgatgtcg ggcaagggct ggcaatggtg atagccagaa acggcaaagc
    11161 gattctctat gacacgggac tggcctggcc tgaaggggat agtgggcaac aactgattat
    11221 cccctggctc cactggcata atcttgaacc ggaaggcgtt atcctgagtc atgaacatct
    11281 ggatcaccgg ggagggctgg actcaatatt gcatacatgg ccgatgttat ggatcagaag
    11341 tccgttaaac tgggaacatc atcagccctg tgtgcgtggc gaagcgtggc aatggcaagg
    11401 attgcgtttc agcgcgcact ggcctttaca aggtcgcaac gataaaggaa ataaccattc
    11461 ctgtgtggtt aaggttgatg acgggacgaa tagcattctt ctaaccggtg atattgaagc
    11521 cccagctgaa caaaagatgc taagccgtta ctggcagcaa gtgcaggcaa cattgcttca
    11581 ggtacctcac catggcagta atacctcatc atcattgcca ttaattcagc gagtgaatgg
    11641 aaaagtggca ctcgcatcgg catcgcgcta taacgcatgg cgactgccct ctaacaaagt
    11701 taagcatcgc tatcaacaac aaggttatac gtggcttgat actcctcatc aggggcaagt
    11761 aacggtcgat ttttcagcgc aaggctggcg gattagcagc ctcagggagc aaattttacc
    11821 tcgttggtat catcagtggt ttggcgtgcc agtggataac gggtagaata tgcggctatt
    11881 tcaacaaatg ctggtttttt gaatgcataa cgacaaagat ctctctacgt ggcagacatt
    11941 ccgccgactg tggccaacca ttgcgccttt taaagcgggt ctgatcgtgg cgggcgtagc
    12001 gttaatcctc aacgcagcca gcgatacctt catgttatcg ctccttaagc cacttcttga
    12061 tgatggcttt ggtaaaacag atcgctccgt gctggtgtgg atgccgctgg tggtgatcgg
    12121 gctgatgatt ttgcgtggta tcaccagcta tgtctccagc tactgtatct cctgggtatc
    12181 aggaaaggtg gtaatgacca tgcgtcgccg cctgtttggt cacatgatgg gaatgccggt
    12241 ttcattcttt gacaaacagt caacgggtac gctgttgtca cgtatcacct acgattccga
    12301 acaggttgct tcttcctctt ccggcgcact gattactgtt gtgcgtgaag gtgcgtcgat
    12361 catcggcctg ttcatcatga tgttctatta cagttggcaa ttgtcgatca ttttgattgt
    12421 gctggcaccg attgtttcga ttgcgattcg cgtagtatcg aagcgttttc gcaacatcag
    12481 taaaaacatg cagaacacca tggggcaggt gaccaccagc gctgaacaaa tgctgaaagg
    12541 ccataaagaa gtattgattt tcggtggtca ggaagtggaa acgaaacgct tcgataaagt
    12601 cagcaaccga atgcgtcttc aggggatgaa aatggtttca gcctcttcca tctctgatcc
    12661 gatcattcag ctgatcgcct ctttggcgct ggcgtttgtt ctgtatgcgg cgagcttccc
    12721 aagtgtcatg gatagcctga ctgccggtac gattaccgtt gttttctctt caatgattgc
    12781 actgatgcgt ccgctgaaat cgctgactaa cgttaacgcc cagttccagc gcggtatggc
    12841 ggcttgtcag acactgttta ccattctgga cagtgagcag gagaaagacg aaggtaagcg
    12901 cgtgatcgag cgtgcgactg gcgacgtgga attccgcaac gtcaccttta cttatccggg
    12961 acgtgacgta cccgcattgc gtaacatcaa cctgaaaatt ccggcaggga aaacggtcgc
    13021 tctggttgga cgctctggtt caggtaaatc aaccatcgcc agcctgatca cgcgttttta
    13081 cgatattgat gaaggcgaaa tcctgatgga tggtcacgat ctgcgcgagt ataccctggc
    13141 gtcgttgcgt aaccaggttg ctctggtgtc gcagaatgtc catctgttta acgatacggt
    13201 tgctaacaac attgcttacg cacgaactga acagtacagc cgtgagcaaa ttgaagaagc
    13261 ggcgcgtatg gcctacgcca tggacttcat caataagatg gataacggtc tcgatacagt
    13321 gattggtgaa aacggcgtac tgctctctgg cggtcagcgt cagcgtattg ctatcgctcg
    13381 agccttgttg cgtgatagcc cgattctgat tctggatgaa gctacctcgg ctctggatac
    13441 cgaatccgaa cgtgcgattc aggcggcact ggatgagttg cagaaaaacc gtacctctct
    13501 ggtgattgcc caccgcttgt ctaccattga aaaggcagac gaaatcgtgg tcgtcgagga
    13561 tggtgtcatt gtggaacgcg gtacgcataa cgatttgctt gagcaccgcg gcgtttacgc
    13621 gcaacttcac aaaatgcagt ttggccaatg atcgaaaaaa tctggtctgg tgaatcccct
    13681 ttgtggcggc tattgctgcc actctcctgg ttgtatggcc tggtgagtgg cgcgatccgt
    13741 ctttgctata aactaaaact gaagcgcgcc tggcgtgccc ccgtaccggt tgtcgtggtt
    13801 ggtaatctca ccgcaggcgg caacggaaaa accccggtcg ttgtctggct ggtggaacag
    13861 ttgcaacagc gcggtattcg cgtgggggtc gtatcgcggg gatatggtgg taaggctgaa
    13921 tcttatccgc tgttattgtc ggcagatacc accacagcac aggcgggtga tgaacctgtg
    13981 ttgatttatc aacgcactga tgcgcctgtt gcggtttctc ccgttcgttc tgatgcggta
    14041 aaagccattc tggcgcaaca ccctgatgtg cagatcatcg taaccgacga cggtttacag
    14101 cattaccgtc tggcgcgtga tgtggaaatt gtcgttattg atggtgtgcg tcgctttggc
    14161 aatggctggt ggttgccggc ggggccaatg cgtgagcgag cggggcgctt aaagtcagtt
    14221 gatgcggtaa tcgtcaacgg cggtgtcccc cgcagcggtg aaatccccat gcatctgctg
    14281 ccgggtcagg cggtgaattt acgtaccggt acgcgttgtg acgttgctca gcttgaacat
    14341 gtggtggcga tggcagggat tgggcatccg ccgcgctttt ttgccacgct gaagatgtgc
    14401 ggcgtacaac cggaaaaatg tgtaccgctg gccgatcatc agtctttgaa ccatgcggat
    14461 gtcagcgcgt tggtaagcac cgggcaaacg ctggtaatga ctgaaaaaga tgcggtgaaa
    14521 tgccgggcct ttgcagaaga aaattggtgg tatttgcccg ttgacgcaca gctttcaggt
    14581 gatgaaccag cgaaactgct tgcgcaacta acctcgctgg cttctggcaa ctagttacgc
    14641 cgcggcagcg ttcgattgat ggagtcatga atgtcgctgc cgcacctctc ccttgctgat
    14701 gcgcgtaatc ttcaccttgc cgcgcaaggc ctgttaaaca aaccccgccg tcgagcgtcg
    14761 ttggaggata ttccggcaac gatctcccgc atgtccttgc tgcaaatcga taccatcaat
    14821 attgttgccc gtagcccata tctggtgctt ttcagtcgtc tgggaaatta tcctgcccag
    14881 tggctggatg agtctctggc gcgtggcgaa ttaatggaat actgggcgca tgaagcctgc
    14941 tttatgccac gcagtgactt tcgtcttatt cgccaccgca tgctggcacc tgaaaaaatg
    15001 ggctggaaat acaaagacgc ctggatgcag gaacatgagg cggaaattgc acagttaatt
    15061 cagcatattc atgataaggg gccggtacgt tcagccgatt ttgagtatcc tcgtaaaggt
    15121 gcaagcggct ggtgggaatg gaagccgcat aaacggcatc tggaaggttt atttactgcc
    15181 ggaaaggtga tggtgattga acggcgcaac ttccagcgcg tttatgattt aacccaccgt
    15241 gtcatgcctg actgggatga tgagcgcgat ctcgtttcgc aaacagaagc agaaatcatc
    15301 atgctggata acagtgcgcg tagccaggga atattccgcg aacagtggct ggcagattac
    15361 tatcggctga aacgtccggc actggcagcg tggcgcgaag cgagggctga acagcggcaa
    15421 atcattgctg tgcatgttga aaaattgggc aatctttggc tgcatgctga tttgctgccg
    15481 ctactcgagc gagcgctggc cggaaagctc actgcaacgc acagcgcggt actttcgcct
    15541 tttgatcctg ttgtctggga tcgcaaacgc gcagagcagc tttttgattt tagctaccgg
    15601 ctggagtgct ataccccagc gccgaaacgc cagtatggct attttgttct gccgttatta
    15661 catcgtgggc aattagttgg gcgaatggat gccaaaatgc atcgccagac aggcatcctt
    15721 gaagttatct ctctgtggtt acaggaaggt attaaaccaa cgacaacgct gcaaaaaggg
    15781 gttacgtcag gcgattactg atttcgctaa ctggcagcag gcaacgcggg tgacattagg
    15841 acactgcccg caaggtctct ttacggattg ccgcaccggc tgggaaatag accccgtcgc
    15901 ataaatgatt atgataagat ttatgcattc ataacccgat ccatctggag gaactatgga
    15961 tcatcgtctg cttgaaatca ttgcctgccc ggtttgcaac ggaaaacttt ggtataacca
    16021 ggaaaaacaa gaacttattt gcaaactgga caacctggct ttcccgctgc gcgatggcat
    16081 tccagtgttg ctggaaacgg aagcccgcgt gctgactgct gatgagagta aatcatgagt
    16141 tttgtggtca ttattcccgc gcgctacgcg tccacgcgtc tgcccggtaa accattggtt
    16201 gatattaacg gcaaacccat gattgttcat gttcttgaac gcgcgcgcga atcaggtgcc
    16261 gagcgcatca tcgtggcaac cgatcatgag gatgtcgccc gcgccgttga agccgctggc
    16321 ggtgaagtat gtatgacgcg cgccgatcac cagtcaggga cagaacgctt ggcggaagtt
    16381 gtcgaaaaat gcgcgttcag cgacgacacg gtgatcgtta atgtgcaggg tgatgaaccg
    16441 atgatccctg cgacaatcat tcgtcaggtt gctgataacc tcgctcagcg tcaggtgggt
    16501 atggcgactc tggcggtgcc aatccacaat gcggaagaag cgtttaaccc gaatgcggtg
    16561 aaagtggttc tcgacgctga agggtatgca ctgtacttct cacgcgctac cattccgtgg
    16621 gatcgtgatc gttttgcaaa aggccttgaa accgttggcg ataacttcct gcgtcatctt
    16681 ggtatttatg gctaccgtgc aggctttatc cgtcgttacg tcacctggca gccaagcccg
    16741 ttagaacaca tcgaaatgtt agagcagctt cgtgttttgt ggtacggcga aaaaatccat
    16801 gttgctgtcg cgcatgaagt tcctggcaca ggtgtggata cccctgaaga tctcgagcgc
    16861 gttcgcgctg aaatgcgcta atttcacttc acgacacttc agccaatttt gggaggagtg
    16921 tcgtaccgtt acgattttcc tcaatttttc ttttcaacaa ttgatctcat tcaggtgaca
    16981 tcttttatat tggcgctcat tatgaaagca gtagctttta tgagggtaat ctgaatggaa
    17041 cagctgcgtg ccgaattaag ccatttactg ggcgaaaaac tcagtcgtat tgagtgcgtc
    17101 aatgaaaaag cggatacggc gttgtgggct ttgtatgaca gccagggaaa cccaatgccg
    17161 ctaatggcaa gaagctttag tacgcccgga aaagcccgac aactggcatg gaaaaccacc
    17221 atgctggcaa gaagtgggac tgtccgtatg ccgactattt atggtgtgat gacgcatgaa
    17281 gaacaccccg gccctgatgt cctgctactg gagcggatgc gtggtgtttc ggtagaggca
    17341 ccagcccgaa caccagaacg ttgggaacag ctcaaagacc aaatcgttga agccttactg
    17401 gcctggcacc gtcaggacag tcgcggttgc gtcggcgcgg tcgacaatac tcaggaaaat
    17461 ttctggccct catggtaccg gcaacatgtt gaagtgctat ggaccacgct caatcagttc
    17521 aataacaccg gtctgacgat gcaggataag cggatcctgt ttcgcactcg cgaatgtctc
    17581 ccggcattat ttgaaggttt taacgacaat tgtgtgctga ttcacggtaa cttctgttta
    17641 cgcagcatgt tgaaagattc gcgcagcgat cagttactgg cgatggtcgg gccgggacta
    17701 atgctttggg caccgcgaga atacgaactg ttccgactaa tggataattc tctggcggaa
    17761 gatttgctct ggagttacct gcaacgcgcg ccagtggcgg agtcgttcat ctggcggcgt
    17821 tggttgtatg tgttatggga tgaagttgcg caactggtta ataccggacg atttagtcgg
    17881 cgcaacttcg atctggcatc aaaatcactc ttgccgtggc tcgcctgacg aaccttttag
    17941 ccactgccag atgcggccaa gcgtttcata gccaacacga tcgctatgca tcaaccagac
    18001 cggagaaggg attgcccgtt cccacgggtt aagcggcgag tcgatggcca actgattcgc
    18061 cggggctggt agtggattta acccttcctg ctgaaaaaag atcatcgccc gcggcagatg
    18121 tgatgcggag gtcaccagca ggaaaggggc gtcaccaatc gcctgtttca ctgccgcagc
    18181 ttcttcttcg gtatcttttg gcaaatccag ggtgataatt tgctcgcgcg gtacgcccag
    18241 cgattgcgca actctggcac ctacttccgc tgtactcacc gtattggttt ttgctacgcc
    18301 tcccgtgaag atcagttttg aacccggatt ttcgcgccat aaacggatac cctcattcag
    18361 gcgtggcaga ctgttattga ttaaattaga gctcggtgcc cactgcgggt tccaggtata
    18421 accacctccg agcaccacga tatagtccac tttttgcgaa ttattccatg tcggataggt
    18481 gctttcgatg ggacgcagca ggcggtcggc taccggttgc aaactcaata acaagagtgc
    18541 cagccaccca atgctaataa aaatcttccc ggttttttga aaacggctaa accacagtag
    18601 cgccaggcca gcgccaataa ttaacaacat cagcggaagt ggcagcaaca tattgccaat
    18661 cacttttttc agtgtaaaaa gcatcctttc tggttccttt tttaaccata tagcaaggga
    18721 tcgccagcga tattacacca gacgggttca ttctcgccgt ggctgtgaca aaatagcgtc
    18781 tttatcagaa aacgtcttag ccaaccggtg gagaaagcaa tgcaggatcg caattttgat
    18841 gatattgcgg aaaagttttc ccgtaacatt tacggcacca ccaaagggca gcttcgacag
    18901 gctattctgt ggcaggatct cgatcgcgtg ctggcggaaa tgggtccgca aaaactgcgt
    18961 gtgctggatg cgggcggtgg agaagggcag accgcaatca aaatggccga gcgcgggcat
    19021 caggtcattt tatgcgatct ttctgcgcag atgatcgacc gcgcaaaaca ggcggcagaa
    19081 gcaaaaggtg tgagcgacaa catgcaattt atacattgcg ccgctcagga tgttgcttcg
    19141 catttggaaa cgcccgttga tctgatattg ttccatgcgg tgctcgagtg ggtggctgat
    19201 ccccgcagcg tattgcagac cctgtggtca gtgttgcgtc caggcggcgt gttgtcgtta
    19261 atgttctaca atgcgcatgg tttgttgatg cataacatgg tcgccgggaa ttttgattac
    19321 gtgcaggcgg gaatgccgaa aaagaaaaaa cggacgcttt cgccagatta tccacgcgac
    19381 ccgacgcagg tttatctgtg gctggaagaa gctggttggc aaattatggg taagacaggc
    19441 gttcgcgtgt ttcatgatta tctgcgcgag aagcaccagc agcgcgactg ctatgaagca
    19501 ttacttgaat tagaaacgcg ttattgccgt caggaaccgt atattaccct ggggcgttat
    19561 attcatgtca ccgcgcgcaa accgcagagc aaggataaag tatgagtgaa ttttcccaga
    19621 cagtccccga actggttgcc tgggccagaa aaaatgactt ctccatttcg ctgccggtag
    19681 accgactctc ttttctgctg gcagttgcca cgctgaacgg cgagcgtctg gatggtgaga
    19741 tgagtgaagg cgagctggtg gatgcattcc gccatgtgag tgatgcgttt gagcaaacca
    19801 gcgaaaccat cggcgtgcgc gccaacaacg cgatcaacga catggtgcgt caacgtctgc
    19861 tgaaccgctt taccagcgag caggcagaag ggaacgcaat ttaccgtctg acgccgctcg
    19921 gcatcggcat tactgactat tacatccgtc agcgcgagtt ttctacgctg cgcctttcta
    19981 tgcagttgtc gattgtggcg ggtgagctca aacgcgcagc agatgccgcc gaagagggcg
    20041 gtgatgaatt tcactggcac cgtaatgtct atgcgccact gaaatattcg gtagcagaaa
    20101 ttttcgacag tatcgacctg acgcaacgtc tgatggacga acagcagcag caggtgaagg
    20161 acgatatcgc ccagttgctg aacaaagact ggcgggcggc gatttccagc tgtgaattgt
    20221 tgctttcgga aacttccgga acgctgcgtg aattgcagga tacactggaa gcggcaggcg
    20281 acaaattgca ggctaatctg ttgcgcattc aggatgcgac gatgactcat gacgatctgc
    20341 atttcgtcga tcgtctggtg ttcgatctgc agagcaaact cgaccgtatt atcagttggg
    20401 gccagcaatc catcgatttg tggattggct acgaccgcca cgtacataaa tttattcgta
    20461 ccgcgatcga tatggataaa aaccgcgtct ttgctcagcg gttacgtcag tcggtacaaa
    20521 cctattttga tgagccgtgg gcgctaagtt atgccaatgc cgatcgtctg ctggatatgc
    20581 gtgacgaaga gatggcactg cgcgatgaag aagtgactgg ggaacttccg ccagatttgg
    20641 aatacgaaga gtttaacgag atccgcgaac agctggcggc gatcatcgaa gaacaacttg
    20701 ccgtgtacaa aaccagacaa gtgccgctgg atcttggtct ggtggtacgc gaatatctgt
    20761 cacagtatcc gcgtgcacgt cactttgacg ttgcgcgcat tgttattgat caggcggtac
    20821 gtcttggcgt agcgcaagca gatttcaccg gactgccagc gaaatggcag ccgattaatg
    20881 attacggagc caaggtacag gcgcatgtca tcgacaaata ttgaacaagt gatgccggtt
    20941 aagctggcgc aggcgctggc gaatccgtta tttccggcgc tggacagcgc cttacgttca
    21001 ggccgccata ttggcctcga cgaactggat aatcatgcat tcctgatgga ttttcaggaa
    21061 tatctggaag agttttacgc gcgttataac gttgagctta ttcgtgcacc ggaagggttc
    21121 ttctatttac gtccacgttc caccacgctg atccctcgtt ccgtcttgtc ggaactggat
    21181 atgatggtcg ggaaaatcct ctgttatctc tatctcagcc cggaacggct ggcgaatgag
    21241 gggattttca cccagcagga actgtacgac gaactgctca ccctggccga tgaagcgaaa
    21301 ctgctgaaac tggtgaacaa ccgttcaacc ggttcagaca ttgaccgtca gaagttgcag
    21361 gaaaaagtac gttcttcgct caaccgtctg cgtcgtttag gtatggtgtg gtttatgggc
    21421 cacgacagca gcaagttccg cattaccgaa tcggtgttcc gcttcggagc cgatgtgcgt
    21481 gctggcgacg atccccgtga agcacagcgt cgcctgattc gtgatggcga agcaatgccg
    21541 attgaaaatc atctgcaact caacgatgaa accgaagaga atcagccaga tagcggagag
    21601 gaagaataat gattgaacgc ggtaaatttc gctcactgac gctgattaac tggaacggct
    21661 tctttgcccg aacttttgac cttgacgagc tggtcacgac gctttctggc ggtaacgggg
    21721 cgggtaaatc caccaccatg gcggcgttcg ttacggcgct gatccccgac ctgaccctgc
    21781 tgcatttccg taacactacg gaagccgggg ccaccagcgg ttcgcgcgat aaaggtctgc
    21841 acggtaagct gaaagcgggt gtctgttatt cgatgctcga caccattaac tcgcgccacc
    21901 agcgcgtggt ggtcggtgtg cgtctgcaac aggttgccgg acgcgatcgt aaagtggata
    21961 tcaaaccatt tgccattcag ggactgccga tgtcggtgca gccgacacag ctggtgaccg
    22021 aaactctgaa cgaacgccag gcgcgcgtgc tgccgcttaa cgagctgaaa gacaagctcg
    22081 aggcgatgga aggcgtgcag tttaaacagt tcaactccat taccgattac cactcgctga
    22141 tgttcgatct gggcatcatt gcgcgtcgtc tgcgctccgc atctgaccgt agcaaattct
    22201 atcgtctgat cgaagcttcg ctgtatggcg ggatctccag tgccattacc cgttctctgc
    22261 gcgactacct gttgccggaa aacagcggcg tgcgtaaagc gttccaggac atggaagcgg
    22321 cgctgcgtga aaaccgcatg acgctggaag cgattcgtgt cacccagtcg gaccgtgacc
    22381 tgtttaagca tctgatcagc gaagctacca actacgtggc ggcggactac atgcgtcacg
    22441 ccaacgagcg ccgtgtccat ctcgataagg ccctggagtt tcgtcgcgaa ctacatactt
    22501 cgcgtcagca actggcggcg gagcagtaca aacacgtcga tatggcgcgt gagctggcag
    22561 agcacaacgg tgccgaaggc gatctggaag cggattatca ggcggccagc gatcacctga
    22621 acctggtgca aaccgcactg cgtcagcagg agaagatcga acgctacgaa gcggatctcg
    22681 atgagctgca gatccgtctg gaagagcaaa acgaagtggt ggcagaagcc atcgaacgcc
    22741 aggaagagaa cgaggctcgc gcggaagctg ccgaactgga agtggacgag ctgaaaagcc
    22801 agcttgctga ctaccagcag gcgctggacg tccagcaaac gcgcgcgatc cagtacaacc
    22861 aggcgattgc tgcgcttaat cgtgccaaag aactgtgcca tctgccggac ttaaccgccg
    22921 acagcgccgc cgaatggctg gaaaccttcc aggcgaaaga gctggaagcg actgaaaaaa
    22981 tgctctctct tgagcagaaa atgagcatgg cgcaaaccgc gcacagccag tttgagcagg
    23041 cttatcagct ggtggtggca atcaacggcc cgctggcgcg taacgaggcg tgggatgtcg
    23101 ctcgcgaact attgcgcgaa ggggtcgatc agcgtcacct ggcagagcag gttcagccgt
    23161 tgcggatgcg cttaagcgaa ctggaacagc gtctgcgcga acagcaagaa gctgagcgtc
    23221 tgctggcaga tttctgcaaa cgtcagggca agaattttga cattgacgag ctggaagccc
    23281 tgcatcagga gctggaagca cgcattgcct ctctttccga tagcgtgtct aacgcccgtg
    23341 aagagcggat ggcactgcgc caggagcagg aacagctgca gtctcgcatt cagagtttga
    23401 tgcagcgtgc accggtttgg ctggcagcgc aaaacagtct caaccagttg agcgaacagt
    23461 gcggcgaaga gttcacctcc agccaggacg tcactgaata tctgcaacag ttgctggagc
    23521 gtgagcgcga ggccattgtt gaacgcgatg aagtaggcgc gcgcaaaaac gccgtcgatg
    23581 aagagatcga acgtttaagc cagcctggcg gctctgaaga tcagcgtctg aacgcgctag
    23641 cggagcgttt tggtggtgtg ctgctgtcag aaatttatga cgacgttagc ctggaagatg
    23701 cgccgtactt ctcagcgctg tatggcccgt cacgccacgc catcgtggtg ccagatctgt
    23761 cacaggtaac cgaacacctg gaaggtttga ccgattgccc ggaagatctc tatttgatcg
    23821 aaggggatcc gcagtcattc gatgacagcg tgttcagcgt tgatgagctg gaaaaagcgg
    23881 tagtggtgaa aatcgccgat cgtcagtggc gttattcacg tttcccggaa gtgccgctgt
    23941 ttggtcgtgc tgcgcgtgaa agccgtattg aaagcctcca tgccgagcgt gaagtgcttt
    24001 ccgaacgctt cgccacgctc tcctttgatg tacagaaaac ccagcgtctg catcaggcgt
    24061 tcagccgctt tatcggcagt catctggcgg tggcgtttga gtctgacccg gaagcagaaa
    24121 tccgtcaact gaacagccgt cgcgtcgaac tggagcgggc gttgagtaat catgaaaatg
    24181 ataaccagca gcagcgtatt cagtttgagc aggcgaaaga aggcgttacg gcgctgaacc
    24241 gcattctgcc gcgtctcaac ctgttggctg atgacagcct ggcggatcgc gtcgatgaaa
    24301 tccgcgaacg tctggatgaa gcccaggaag ccgcgcgttt tgttcagcag tttggcaatc
    24361 aactggcgaa actggaaccg atcgtttcgg tattgcagag cgacccggaa cagttcgaac
    24421 agttaaaaga agattacgcg tactctcagc agatgcagcg cgatgcccgt cagcaggcgt
    24481 ttgccctgac ggaagtggtg cagcgtcgtg cgcactttag ctactctgac tcggcagaaa
    24541 tgcttagcgg taacagcgat ctcaacgaaa aactgcgtga acgtctggaa caggcggaag
    24601 cggagcgtac ccgcgctcgc gaagcgttgc gcggtcacgc agcgcagttg agtcagtaca
    24661 accaggtgct ggcttcgctg aaaagttctt acgacaccaa aaaagagcta ctcaacgatc
    24721 tgcaacgtga attgcaggat atcggcgtgc gtgctgatag cggggcagaa gagcgggcac
    24781 gtattcgccg tgacgagctg catgcacaac tgagcaataa ccgttcgcgc cgcaatcaac
    24841 tggaaaaagc gctcaccttc tgcgaagcgg agatggataa cctgacccgc aaactgcgca
    24901 agctggagcg tgattacttt gagatgcgcg agcaggtagt gaccgcgaaa gcgggctggt
    24961 gtgcagtcat gcgcatggtg aaagataacg gcgttgagcg ccgcttacac cgtcgtgagc
    25021 tggcttatct ctccgctgat gatttgcgtt ccatgtcgga taaggcgtta ggtgcgctgc
    25081 gtctggcggt ggcggataac gaacatctgc gcgacgtgct gcgcatgtcg gaagatccca
    25141 aacgtccgga gcgtaaaatt cagttcttcg tggcggttta tcagcatctg cgtgaacgta
    25201 ttcgtcagga tattattcgt accgatgatc cggtggaagc tatcgaacag atggagatcg
    25261 aacttagccg tctgaccgaa gagttaacct cccgtgaaca gaaactggcg atcagttccc
    25321 gcagcgtggc gaacatcatt cgcaaaacca ttcagcgcga gcagaaccgt atccgtatgc
    25381 tcaaccaggg gttgcagaac gtatcgtttg gtcaggtgaa cagcgtgcgc ctcaacgtga
    25441 acgtgcgtga aacgcacgcc atgctgctgg atgtgctctc tgaacagcat gagcagcatc
    25501 aggatctgtt taacagcaac cgtctgacct tctcggaagc gctggcgaaa ttgtatcagc
    25561 gtcttaatcc gcagattgat atggggcagc gcacgccgca gaccattggc gaagaactgc
    25621 tggattaccg caactatctg gaaatggaag ttgaggttaa ccgtggttcc gatggctggt
    25681 tgcgcgcaga gtctggtgca ttgtcgaccg gtgaggcgat tggtaccggt atgtcgattc
    25741 tggtgatggt ggtacaaagc tgggaagatg aatctcgccg cctgcgcggt aaagatatct
    25801 ctccttgccg cctgctgttc ctcgatgaag cagcgcgact ggatgctcgt tctatcgcca
    25861 cgctgtttga attgtgtgag cgtttgcaaa tgcagctcat catcgcagcg ccggaaaata
    25921 tcagcccgga gaaaggcacc acctataaac tggtgcgtaa agtcttccag aataccgaac
    25981 atgttcatgt cgtcggcctg cgaggatttg cgccgcaact ccctgaaacg cttccaggaa
    26041 gtgacgaagc gccttctcag gcgagttaaa attaaggcgg cagcaatgcc gccttttctt
    26101 tttccgaaaa ctccgtttct gcactaaaaa agtggcacat tacggcgcgc ttttctttaa
    26161 acttctttac attaggttat gtaaaaacgt atcggcgttt atatactgaa gataagcctg
    26221 atgagtaaca ggcttgctcg tcatactttc gtgagtattg gcgttgtaca ggcaagtcgt
    26281 aaaataacag cctggctatt cagagtatga taaaaacagg gggcaaggga tgttgcttaa
    26341 tatgatgtgt ggtcgtcggc tgtcggcaat cagtttgtgc ctggccgtaa cattcgctcc
    26401 actgttcaat gcgcaggccg atgagcctga agtaatccct ggcgacagcc cggtggctgt
    26461 cagtgaacag ggcgaggcac tgccgcaggc gcaagccacg gcaataatgg cggggatcct
    26521 gccattgcct gaaggtgcgg cagaaaaagc ccgcacgcaa atcgaatctc aattacccgc
    26581 aggttacaag ccggtttatc ttaaccagct tcaactgttg tatgccgcac gcgatatgca
    26641 acccatgtgg gaaaaccgtg atgctgttaa agccttccag caacagctgg cagaggtggc
    26701 gattgccggt ttccagccgc agtttaataa atgggtagag ttactgaccg atcctggtgt
    26761 taacgggatg gcacgcgacg tggtgctctc tgatgcgatg atgggctatc tccatttcat
    26821 tgcaaatatt ccggtcaaag gcactcgctg gctatatagc agtaaacctt atgcgcttgc
    26881 aacgccgccg ctttcggtga ttaaccaatg gcagcaggcg ctggataaag gtcaattgcc
    26941 tacgtttgtt gcaggactgg caccgcagca tccgcaatat gcggtgatgc atgaatcgtt
    27001 actggcctta ctcagtgaca ccaaaccgtg gccccaactg accggcaaag caacgttgcg
    27061 cccagggcag tggagtaacg acgtaccggc gttgcgtgaa atattgcaac gcacaggcat
    27121 gttggacggg gggccgaaaa ttactctacc tggcgatgac acgccaactg acgcggtagt
    27181 cagcccatcc gctgttactg ttgaaacagc agaaactaag ccgatggata agcaaacgac
    27241 gtctcgtagt aaacctgcgc ctgccgttcg cgccgcctac gataatgaac tggtggaagc
    27301 cgttaaacgt tttcaggcat ggcaaggatt gggggcagat ggtgctattg gcccggcaac
    27361 gcgtgactgg ttaaacgtaa cgcccgccca gcgtgctggc gtgttggctc tcaacatcca
    27421 gcgattgcgc ttgctgccaa cagagctttc taccgggatc atggttaaca ttccggccta
    27481 ttcgctggtc tactatcaga acggcaatca ggtgctggat tcgcgagtca ttgtcggtcg
    27541 ccccgatcgc aaaacgccga tgatgagcag tgcccttaac aatgtagtgg taaacccgcc
    27601 gtggaacgta cctccaactc tggcacgcaa agatattctg ccgaaagtgc gcaacgatcc
    27661 gggatatctc gaaagccatg gctatacggt gatgcgcggc tggaacagca gagaagcgat
    27721 tgacccatgg caggttgact ggtctacaat cacggcctcg aatttaccgt ttcgcttcca
    27781 acaggctcca ggcccacgga actcgctggg gcgctataaa ttcaatatgc cgagttcaga
    27841 ggccatttat ttgcatgaca cgccgaacca caatctgttc aagcgtgata cacgcgcatt
    27901 gagctcaggc tgtgtacgag tgaataaagc ttccgatctg gcgaatatgc tgttgcagga
    27961 tgcaggctgg aatgacaaac gtatttctga tgcgctgaag cagggtgata cacgttacgt
    28021 caatattcgg cagtcgattc cggtgaatct ctactacctg acggcctttg ttggtgcaga
    28081 tggtcgtacc cagtatcgta cagatattta caattatgat ctgcctgcgc gatccagctc
    28141 gcaaatcgta tcgaaagcgg aacaattaat caggtaaatg aagtagttcg gggaaatcag
    28201 ttgtcgtaat aattagcatg attgggggca attctctgca gcccccgtca ctgctggggt
    28261 tgagtcatct tgacgtctgc tttacgggcg gttaaggtgc ctcttgtgcg ccagaagtgc
    28321 atataaacga taacattgac ctgtagactt gattatcatg gacaaattcg acgctaatcg
    28381 ccgcaaattg ctggcgcttg gtggcgttgc actcggtgcc gccatcctgc cgacccctgc
    28441 gtttgcaaca ctctctaccc cacgcccgcg cattttgaca ctcaataatc ttcataccgg
    28501 agagtcaatc aaagcggagt ttttcgatgg cagaggctat attcaggaag aattggcaaa
    28561 acttaaccat tttttccgcg attaccgcgc gaacaaaata aagtccatcg acccaggatt
    28621 attcgaccag ttgtatcgcc tgcaagggtt attaggcacc cgcaaaccgg tgcaactcat
    28681 ttccggttat cgttccattg ataccaacaa tgaactacgc gcccgcagcc gtggagtagc
    28741 gaagaaaagc tatcacacta aaggccaggc gatggatttc catattgaag gtatcgcgtt
    28801 aagcaatatt cgcaaagccg cgttatctat gcgcgcaggt ggtgtaggat attacccacg
    28861 tagtaacttt gtgcatattg ataccgggcc agcacggcac tggtagtaat tgcttaacga
    28921 aacaggggca gtatgaacta tcgtattatt ccggtcaccg cattctccca gaactgttca
    28981 ttgatctggt gtgaacaaac ccgcctggcc gcactggtcg atcctggcgg cgatgcggaa
    29041 aaaatcaaac aggaagttga cgccagcggc ctgacactga tgcagatcct gctgacgcat
    29101 ggtcatctgg accacgttgg cgcagcggcg gaactggcgc aacattacgg cgtgccggtt
    29161 tttggcccgg aaaaagaaga tgagttctgg ctgcaaggct tgcctgcgca aagtcgtatg
    29221 tttggtctgg aagagtgcca gccgctgacg ccagatcgtt ggctgaacga aggcgatacc
    29281 atcagcatag ggaatgtgac tttacaggtg ttacattgcc ctgggcatac gccgggtcat
    29341 gtcgtgtttt ttgatgatcg ggcaaagctg ctgatttctg gcgatgttat tttcaaaggc
    29401 ggagtagggc gcagtgactt cccgcgtggc gatcataatc aactgatttc ttcaatcaaa
    29461 gataaattgc tgccactggg tgatgacgtg acatttattc cgggtcacgg accattatcc
    29521 acacttggtt atgaacgcct gcataatccc ttcctgcaag acgaaatgcc cgtctggtaa
    29581 ggcacacata aaaaagcccg cttttcagcg ggcttcattg tttttaacgc ttacagcact
    29641 gccacaatcg cttcgcacag cggagccatg ttatctggtg tcatcccggc cacgtttacg
    29701 cgaccagaag caacagcata cacgccaaac tcttcgcgca gacgcagcac ttgttctttt
    29761 gtcaggccac tgaaggagaa catgccgttc tgtttgatga taaagctgaa gtcgcggttt
    29821 gcgccttttt cctgcagcgt attgacgaac aactgacgca tacgctgaat acgctggcgc
    29881 atatcagtca gctcttgttc ccaaatcgca cgtaacgcat cgttgctcag gatggtggca
    29941 acaacagaag cgccgtgtgc tggtgggtta gagtagttag cgcgaatcgc cgctttcatt
    30001 tggctgaatg cgcgatcaac ggtttcactg tcggcagcaa ccagagtaca agcgccaaca
    30061 cgctcgttgt acaggccaaa gtttttagag taggaactgg caacaatcag ctctttatgc
    30121 atagccgcga aagcgcgcag tccttcagca tcttcttcca gaccacgggc aaaaccctgg
    30181 taagcgaagt caaacagcgg taaccagcct ttctcaacgg agagttgtgc cagtgtctgc
    30241 cattgttcca gcgtagggtc gataccggtt gggttgtggc agcagccatg gaacagcact
    30301 acgtcgccag cctgagcttc gttcaggctg ttaatcagtg catcgaagtc aagggtgtgg
    30361 ttttccgcat cataataagc gtattcacga acttccagat ctgcagagtt aaagacgctc
    30421 ttatggttcg gccagcttgg gttgctcacc cacactcgct taacgctggt attttttgcc
    30481 aggaaatcgg cagctatgcg tagtgcgcca gtgccacccg gagtctgtgc cgtgcgagca
    30541 cgtttgtcat tgatcagggc gctaccttta ccaaacagca gttcctgagt gcagcgacca
    30601 aattcaggga tgccgtcaat gccgaggtaa tttttggtgg tttcattttc gagcagatac
    30661 tgttcagcct ttttcacgct ggtcagtacc ggggttttgc ccgtctcatc tttatagaca
    30721 ccaatcccga ggttaatttt gccgggacgt tcatcggcac gaaacagatc ggccaggccc
    30781 agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca tgacgaggtt ccattatggt
    30841 tacagaaggg aagtccgcta tcagggtaac gggagattta caaaattcca actattactg
    30901 atgaaaacgc aggctgtttt tgcaagacgt gagattgctc tggaaggcat aaaaaaaaca
    30961 ggaccaaagt cctgtttttt cggcatttaa caaagaggtg tgctattaga actggtaaac
    31021 gatacccaca gcaacggtgt cgtctgaacc tacgcccagt ttgttgtcag aatcgatctg
    31081 gttgatgatg tagtcaacat aggtggacat gtttttgttg aagtagtagg ttgcgcccac
    31141 ttcaaagtag ttcaccagat caacatcacc gataccttct acgtctttcg ctttagattt
    31201 ggtgtaagcg atggacggac gcagaccgaa atcgaactgg tattgcgcaa ctaacagaac
    31261 gtcttgcgtt ttgttggcga agccgctgat gtttgtaaat ttattagtga tcggcgtagc
    31321 gttacgcgtt tcaccgtagt tcgctgccag gtagatgttg ttcgcatcat acttcagacc
    31381 agtagcccac tgttcagctt ttttaccgtt gccaagaagt tgagcttctt gcaggttggt
    31441 acggtcagct gcaccataag caccaacgat accaaagcct tcgtattcgt agctgataga
    31501 accgccaaca ccgtcgccgt tagagcggcg tgcagtgtca cgctcgtttt tacccaggta
    31561 ctgaacagcg aagttcaggc catcaaccag accaaagaag ttggagttac gataggtagc
    31621 aacgccgcca acacgaccaa cgaagaagtc atcgctgtat gcagtatcac caccaaattc
    31681 tggcagcata tcggtgtaac ccagtgcatc ataaaccaca ccgtagttac ggccgtaatc
    31741 gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc agacgcgttt tgttaccagt
    31801 ttgagcgtca gcgccttcag agttgttacc ctggaagtta tattcccact gaccataacc
    31861 ggtcagatcg gaattgattt gagtttcccc tttaaaacca agacgggcat aggtcatgtc
    31921 gccattgcca ccgtaactgt tttcaccgtt acccttggag aaataatgca gaccgacagc
    31981 tttaccgtac agatctactt tgttgccatc tttgttatag atttctgcag cgtttgcagt
    32041 acctgctact aacagagcag ggacgatcac tgccagaata ttgcgcttca tcattattta
    32101 ttaccctcat ggtttttttt atgacacctg ccactgccgt caataatttc tgtcaataaa
    32161 attttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc atctttccat
    32221 tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat aaagatttag tttcaaaaag
    32281 aaaaaatatg taactaaaag taaaaattaa ggaactttgt gaacaccgtc atatttccat
    32341 a
//
LOCUS       DAZFBR010000049        31821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFBR010000049 DAZFBR010000000
VERSION     DAZFBR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.049"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..337)
                     /gene="gadE"
                     /locus_tag="ACHJZ2_002462"
     CDS             complement(<1..337)
                     /gene="gadE"
                     /locus_tag="ACHJZ2_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO4568550.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNS"
     gene            complement(1136..1708)
                     /gene="hdeD"
                     /locus_tag="ACHJZ2_002463"
     CDS             complement(1136..1708)
                     /gene="hdeD"
                     /locus_tag="ACHJZ2_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO4568551.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1963..2295
                     /gene="hdeA"
                     /locus_tag="ACHJZ2_002464"
     CDS             1963..2295
                     /gene="hdeA"
                     /locus_tag="ACHJZ2_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO4568552.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            2411..2737
                     /gene="hdeB"
                     /locus_tag="ACHJZ2_002465"
     CDS             2411..2737
                     /gene="hdeB"
                     /locus_tag="ACHJZ2_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO4568553.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2801..3448
                     /gene="yhiD"
                     /locus_tag="ACHJZ2_002466"
     CDS             2801..3448
                     /gene="yhiD"
                     /locus_tag="ACHJZ2_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO4568554.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(3500..4270)
                     /locus_tag="ACHJZ2_002467"
     CDS             complement(3500..4270)
                     /locus_tag="ACHJZ2_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO4568555.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAAHYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(4267..5259)
                     /gene="shuU"
                     /locus_tag="ACHJZ2_002468"
                     /gene_synonym="chuU"
     CDS             complement(4267..5259)
                     /gene="shuU"
                     /locus_tag="ACHJZ2_002468"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO4568556.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCAIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(5308..5931)
                     /gene="chuY"
                     /locus_tag="ACHJZ2_002469"
     CDS             complement(5308..5931)
                     /gene="chuY"
                     /locus_tag="ACHJZ2_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO4568557.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(5931..6425)
                     /gene="hutX"
                     /locus_tag="ACHJZ2_002470"
     CDS             complement(5931..6425)
                     /gene="hutX"
                     /locus_tag="ACHJZ2_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO4568558.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(6438..7775)
                     /gene="hutW"
                     /locus_tag="ACHJZ2_002471"
     CDS             complement(6438..7775)
                     /gene="hutW"
                     /locus_tag="ACHJZ2_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO4568559.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            complement(7795..8709)
                     /gene="shuT"
                     /locus_tag="ACHJZ2_002472"
     CDS             complement(7795..8709)
                     /gene="shuT"
                     /locus_tag="ACHJZ2_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO4568560.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGELVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            9392..11374
                     /gene="chuA"
                     /locus_tag="ACHJZ2_002473"
                     /gene_synonym="shuA"
     CDS             9392..11374
                     /gene="chuA"
                     /locus_tag="ACHJZ2_002473"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO4568561.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            11423..12451
                     /gene="chuS"
                     /locus_tag="ACHJZ2_002474"
     CDS             11423..12451
                     /gene="chuS"
                     /locus_tag="ACHJZ2_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO4568562.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(12512..13042)
                     /gene="dctR"
                     /locus_tag="ACHJZ2_002475"
     CDS             complement(12512..13042)
                     /gene="dctR"
                     /locus_tag="ACHJZ2_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO4568563.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(13198..13764)
                     /gene="slp"
                     /locus_tag="ACHJZ2_002476"
     CDS             complement(13198..13764)
                     /gene="slp"
                     /locus_tag="ACHJZ2_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO4568564.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(14118..15233)
                     /locus_tag="ACHJZ2_002477"
     CDS             complement(14118..15233)
                     /locus_tag="ACHJZ2_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568565.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIFSHKEHPTPKLYKDEQYLDKQIEG
                     LFSTLLTINDNFLRAKAEIASTIIKFLEARITNLSYNDILKYQQEFQKECYKQVKSFT
                     TLSRYNKIQTWAEMSEYQFEVFQYETLNPKKCHARLI"
     gene            complement(15860..16285)
                     /gene="arsC"
                     /locus_tag="ACHJZ2_002478"
     CDS             complement(15860..16285)
                     /gene="arsC"
                     /locus_tag="ACHJZ2_002478"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO4568566.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(16298..17587)
                     /gene="arsB"
                     /locus_tag="ACHJZ2_002479"
     CDS             complement(16298..17587)
                     /gene="arsB"
                     /locus_tag="ACHJZ2_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO4568567.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(17641..17994)
                     /gene="arsR"
                     /locus_tag="ACHJZ2_002480"
     CDS             complement(17641..17994)
                     /gene="arsR"
                     /locus_tag="ACHJZ2_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO4568568.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            18738..18821
                     /gene="dinQ"
                     /locus_tag="ACHJZ2_002481"
     CDS             18738..18821
                     /gene="dinQ"
                     /locus_tag="ACHJZ2_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO4568569.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(18875..20227)
                     /gene="gorA"
                     /locus_tag="ACHJZ2_002482"
     CDS             complement(18875..20227)
                     /gene="gorA"
                     /locus_tag="ACHJZ2_002482"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO4568570.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(20299..21141)
                     /gene="rlmJ"
                     /locus_tag="ACHJZ2_002483"
     CDS             complement(20299..21141)
                     /gene="rlmJ"
                     /locus_tag="ACHJZ2_002483"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO4568571.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHTERTGEYLEGIARIWQQDDLPAELEPYINMVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            21344..23386
                     /gene="prlC"
                     /locus_tag="ACHJZ2_002484"
     CDS             21344..23386
                     /gene="prlC"
                     /locus_tag="ACHJZ2_002484"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO4568572.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            23394..24146
                     /gene="rsmJ"
                     /locus_tag="ACHJZ2_002485"
     CDS             23394..24146
                     /gene="rsmJ"
                     /locus_tag="ACHJZ2_002485"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO4568573.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(24195..25664)
                     /gene="dtpB"
                     /locus_tag="ACHJZ2_002486"
     CDS             complement(24195..25664)
                     /gene="dtpB"
                     /locus_tag="ACHJZ2_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO4568574.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGTFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            25830..25883
                     /locus_tag="ACHJZ2_002487"
     CDS             25830..25883
                     /locus_tag="ACHJZ2_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568575.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            25861..25914
                     /locus_tag="ACHJZ2_002488"
     CDS             25861..25914
                     /locus_tag="ACHJZ2_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568576.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(25981..26415)
                     /gene="uspA"
                     /locus_tag="ACHJZ2_002489"
     CDS             complement(25981..26415)
                     /gene="uspA"
                     /locus_tag="ACHJZ2_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO4568577.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            26806..27141
                     /gene="uspB"
                     /locus_tag="ACHJZ2_002490"
     CDS             26806..27141
                     /gene="uspB"
                     /locus_tag="ACHJZ2_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO4568578.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(27212..28711)
                     /gene="pitA"
                     /locus_tag="ACHJZ2_002491"
     CDS             complement(27212..28711)
                     /gene="pitA"
                     /locus_tag="ACHJZ2_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO4568579.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            28943..30145
                     /gene="yhiN"
                     /locus_tag="ACHJZ2_002492"
     CDS             28943..30145
                     /gene="yhiN"
                     /locus_tag="ACHJZ2_002492"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO4568580.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(30462..31514)
                     /gene="yhiM"
                     /locus_tag="ACHJZ2_002493"
     CDS             complement(30462..31514)
                     /gene="yhiM"
                     /locus_tag="ACHJZ2_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO4568581.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         8410 a         7548 c         7827 g         8036 t
ORIGIN      
        1 gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt ttgatacttt
       61 ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg tgataaggtg
      121 ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct tcttcatcaa
      181 ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct acttttttga
      241 tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc caaaagccct
      301 gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta gccgttatcg
      361 tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg ttcaatatag
      421 taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca ctaatcaata
      481 cttcagattt atccatcacg cactccatat attgatccta agtaagattc ctggttgtta
      541 tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca agaataagtt
      601 atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaaagt aatccgattt
      661 aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc tttcttttaa
      721 cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta tatagcgtct
      781 ggagacatgg atatttatgc aatgtaatca acgccagttg caaaaaaatg aacccggatt
      841 acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact ttcccacagc
      901 acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt ttgaaatggc
      961 taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat aatcactagc
     1021 aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc ggagagagta
     1081 ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg gataattatt
     1141 gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat atcagttcaa
     1201 ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca aggaatatcc
     1261 aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct ttcattgaac
     1321 gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa ccggcaataa
     1381 atgccgcaat ggcaaaaatg cccagctccg gtgcgcggat gaagaaatag ccgatcaata
     1441 aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga ctgcggttgc
     1501 tgaataatcc aacaataagc gcaatgcccg aacagattaa taatgcacct actactgtgc
     1561 ttaaaatatc gccagaaacg aacgggaaac tgatacacag caacccgacg ataaacagca
     1621 gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga tcaaacttca
     1681 aaattgttgc cttatctata tataacatag aaccacccta taaaattaag aagaaaatcc
     1741 cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa taaaaatttc
     1801 ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat atctgatttt
     1861 gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag tgtcgaaatt
     1921 gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa agtattaggc
     1981 gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga tgcgcaaaaa
     2041 gcagctgata acaaaaaacc ggtcaactcc tggacctgtg aagatttcct ggctgtggac
     2101 gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa agataaacca
     2161 gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat cgttcaggct
     2221 tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg ggacaaaatt
     2281 aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata ttgaacaggc
     2341 cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc tatattgaat
     2401 gggttacaaa ataaatattt catctctccg taaagcgttt atttttatgg gcgctgtagc
     2461 ggctttgtca ctggtgaacg cacaatctgc gctggcagcc aatgaatccg ctaaagatat
     2521 gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg catggtggat
     2581 gctgcatgaa gaaacagtat ataaaggtgg cgatacagtt actttaaatg aaaccgatct
     2641 cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa atttgtatac
     2701 cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta aaaaggagtg
     2761 gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag aatttattat
     2821 acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc aaatgcgcgg
     2881 caaaggagca gggttacgca ctcatgtatt aattggcatg ggaagcgcac tgtttatgat
     2941 tgtttcgaaa tatggttttg ctgacgtgct gtctttagat cacgtcggac tggaccccag
     3001 ccgtattgct gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta acattctcgt
     3061 tcgtaaccaa aatattgtag gtttgacgac ggcagcggat atctgggtga ccgccgccat
     3121 aggtatggtt attggcagtg gtatgtacga actgggtatt tatggttcag tgatgacctt
     3181 gctggtgctg gaagtcttcc atcaattaac cttccgcctg atgaacaaaa attaccattt
     3241 acagctgaca ttagtgaacg gtaatacggt atcaatgctc gactggttta agcagcaaaa
     3301 aataaaaact gatttagttt cgttgcagga aaatgaagat catgaagtgg tcgccattga
     3361 tatccagctt catgccacaa cgtcgataga agatctgttg cgattattaa aaggtatggc
     3421 aggggtgaaa ggcgtttcaa ttagctaatc tgtatagtta tttattcgca ggagtaccag
     3481 tgcggtactc ctgttgcttt taatgatcga ggacgatcag tggtgaatgg ttggcaggat
     3541 ctttaagtac ggtaatatcc gcgccgtaga gcattgtcag cgcctgctgt gtcaatacgt
     3601 cctgtggttt accatttgcg ataacctttc ctttttgcat taatacaaca cggtctgcat
     3661 aatgcgcggc aagatttaga tcgtgcaata tgcagcagac attgaactgc cgctcgtgaa
     3721 ctaactggcg taataaacga aacaagtgct gctgatggtg aatatccagc gccgaagttg
     3781 gttcatcaag aaataaccat ttcggtgagg gagttggttc ccagagttgc accagcaggc
     3841 gcgctaattg cacgcgctgt tgttcaccac cggataattg ccgatagtcg cggtttgcta
     3901 acgcctgaca gtcacataac gccattattt gtgctgtttc attatcctga ttaccagtac
     3961 gatgcggatg ccgacccatc tggataactt cttgtacgct gaatggaaac gccatgtggc
     4021 tattttgacg catcacggcc cggtgtttcg ccagttcagt aatcgaccat tcatttaatg
     4081 gcttattaaa caaccgacat tcgccgctat caggttgaag atagccggtt aactggcgaa
     4141 gcagcgtaga ttttcccgca ccattagggc cgagaatggc aacaatttcc ccaccgggaa
     4201 aagtcagcga gacattatca gtcagacgcc gcccctgaag gctataaacc agattctggg
     4261 cgctgatcat gtgatcctcc gatttcgcag aatcaaccac ataaaataag ggccaccaag
     4321 caggctggtt aataatccca cgggcatttc tgctggctgt accagcgtgc gggcaagcgt
     4381 gtctgccatc agcaataaac aggctccggc gagggcggaa caagggatga gccagcggtg
     4441 atttgctccg gtagtcatgc gaatcagatg cgggatcacc aggccaataa agccgataat
     4501 gccgcttacc gatacggcag caccaacgag cagtgagctc actaatagta attgctggcg
     4561 tttacgctta acgttcacgc cgaggtaatg ggcttcttca tcaccgagct gcagtaaatt
     4621 cagcgttccg gcgagacaag ttgttgcgat aatggccgga aggatgaatg aactggcaac
     4681 caataacgtc ggccattgcg cctgtccaag attgcccatc atccacaatg tgagttgtcg
     4741 tagctgctgc tcatcgccaa tatagctcag gatgccgatc gctgcgccgc acagggcatt
     4801 gatggcgata ccggcgagta ataattgcat catgctgcca tcgcgatggc gctgcgtgat
     4861 gagaaaaatg atggcgcaga ctactaaact tccggcaatg gcaaacacca tttgctcata
     4921 gagcaccagc accactggga aggagaaggg cagcacaatt gcaacgccaa ccattaatgc
     4981 agaaccgcta ctgacgccga gtaatcctgg gtcagccatt gggttgcgaa aaagcccctg
     5041 cataattacc cctgcggttg ccaacgctgc acctaccagc accgccagca aaacccgtgg
     5101 tagacgaata ttgagccaga tatcgcggta ttcctcatca aaaagggcat caaaactgat
     5161 ctttaacgct ccctgactag caccgaacag caccagggca agcaataata gtgatagccc
     5221 cataaagacc ctggcggatg aaaatgaatc cttgagcacg caaaactccg ttacgacatt
     5281 acggcgcgga catgccgcgc cggggtatca cgccggtttc aggtccggtt caatcagact
     5341 gtagacctgc tggttaagtg ctggcgcatt tgccagttca tggatatgtg cggcgacatc
     5401 ggcacggcgg acaaaaccat gacactcctg attttgtatc cgttgtgctt tgcctgttgc
     5461 tgcgccgtca agcagaccac cagggcggag aatggcgtag tctagctggc tggtttgcag
     5521 ccagctttcc gccagcgttt tttcccgtac cgcctgacca aacgcagcct ttgcccgctc
     5581 cgataaaaac ggccagctat cgccgcatcc caaagaggtc accagaatca tgcgactgat
     5641 gccggccttt tcggcctcat caatcactgt tctgtgcgcc agataatcct gagctcctcc
     5701 cattgttgag atgataagcg catccgggcc agctgcgcgg caagcagccg cgactacgct
     5761 ggcatcacag gcgtcgcctg taaaaacctg tacgccttgt tgcgccagtt tcgtggcggc
     5821 atcggcatga cgaatgacag caacgaccgg acgttgttcc gccagcgcca gttcaagcgt
     5881 tctggcaccc acaccttttc cgcctgcgcc aaagagtagc cacggcgtca tcaggcgtgt
     5941 tcctttaacg atgccgccag agtatggaag gcgctgactt gttcgctcag gagctgacga
     6001 tggtcatccc tgccgaggaa gattttcagc atggcgctgc cttctttgtt gaaaaagaga
     6061 atggacgcgg tgtccatgcc cataaattta cgttcgatta aggcaatatg cgtgcagttt
     6121 tctgctttga tatgcccgga catgccgtgc tttccgcgta ggttgaaata gccgtggcga
     6181 tggaaacccg aaggcagttc gccgctaaat tcgaggatta catcggcagt atgtactaac
     6241 gtggtgacgt tgccccattc gcagacggta tcccagacag tatcgaattt atcgcctggc
     6301 actacggtag aggagggtag atttctcact acctccagca gtgtggtgtt gtattgttcg
     6361 gctaccacct ccagcgtacc gtccggctcc gttttcaaaa attcttgtaa cgagacatgg
     6421 ctcataaatc actcctgtta tggtttttca cgcataatcg ctggcgcatt aagtacctga
     6481 attagttcat taagagactg caaaatattg cttgcccaga aacgaccttc attagtcagc
     6541 cgcaggcaag ttgaagcatc gcgacttaag cctttttggt gccattgagc cagtaacggc
     6601 gcgagttttt cggcatgtgg cgtaagttca tccagcggta cgcgcgctgt ttcaacacct
     6661 gactgcaagg tatgacgcca ttgcgcatta cgttcggcgt tacgcatgat caacatcagc
     6721 ggttttttgc ctgccgcgac ggattcatgc caggtctgaa gattgcgttc gttcatccag
     6781 gagtaaccat taatcgaacc gccggctccg gaaccaaagg ccagacaatc ggcaccttgt
     6841 ttaatcagca ggttatagag attgcgttcg cgtgtggtac ggccccagtg gctgttactg
     6901 atgcagcgcc aaccggcatc gtccataaaa tcacatcctt gcagataaag atcgcgacgt
     6961 tctgccggtg agggtacggt agtacgccca ttttccacgg ccttgcccag cggtgtattg
     7021 gacaggacat tgagcgcata gagatcgacg ccgtcgagac cgatatcgcg ggcaatagcc
     7081 agatcttctc cccaggtttg cgcatcctga cccggcaggc caaacagcag gtcacagacc
     7141 accgcagcac ggtcacgttt gaccaggctt tccataaacg caatagcggt tgggccatcc
     7201 gaggtgcggg ccattttctt gcggattttg ctgttaaacg actggatacc aattgagaag
     7261 cggtttgctc ctgcatcaag gcaagcatcg attcgctcag cgtcaaaatt caatacccgt
     7321 ccttcgatag tgatttcaca atcaggggcc agtggcagct tttctcgtaa tgtggtgatg
     7381 attctggcca aatcgtgtgc cgaaagtgcg gaaggcgtac cgccaccgaa atagaccgcg
     7441 tggatggggg cagactgatg caatacgctg tccgcttcca tttctatttc acgaatcagg
     7501 gcgtcggtat aatgagcaca cgcatcttca ttaaaacggt tctgataaaa accacaaaac
     7561 gtgcagtgtg tcgcacaaaa aggaatgtgt aggtaaacca gtcgcttacg aggtgatgcc
     7621 gtttgattga tcatctcttg ccaggtctgt gccagttgct ctttggcaac cggaatggct
     7681 ccacgaaacg gcatcatggc gcgcctgtct ttaaatggct gatcaccgtc taaagcaaaa
     7741 tgtggcgtga gatccagggt gttatttgcg ttcattcgtc tgatttaaat ccatttattg
     7801 cggccaaagc tgctggtgta aagactcaac gacatcagca atacgcggac ccattcccag
     7861 aatcaggttt tgatcaacgg tgataatgcg ctggtttttc catgcggcag tgtgggtaat
     7921 tccagcaata gaacgtagcc gattaatatc gccatcgacc atttgtgagg ttacgacaat
     7981 cacttctgga ttagccgcaa tcagtgattc tgcactgtag cttttgtact gctggtgggt
     8041 agcgacgttt tctgctccgg caagcgaaag aatggcgtcc gcgacgctgc ctttaccggc
     8101 aacttgtggc gcacttccgc ctgcggagag aataaacatg gctttaaccg gggctttttt
     8161 ggtcgccacg ttttgctgta ctcgctccag gcgttggctg atttgtgtca ccagcgcctc
     8221 gccttgttca ggaacctgta gcgtttttgc cagttggcga atgttggcgt acatctgctg
     8281 aagggtggca ggcacacgcg gcagggtgac gacgttgact ttctgcgctc gtagttggtc
     8341 gagcacaatt tgtggcccgg catcctgcca ggtaattacg ctatccgggc gaagtgacaa
     8401 aatgccttcg ctgctgagtt gtttccagta gccaatatgc ggcagtttgg cggtttctgg
     8461 tggataagat gtcgtttcat cgacaccaac cacgagttcg ccagcgccca tcgcgtagat
     8521 tagctccgtc agcgatcctc ctgcgaccac gatacgttcg gcagccgtta cgctaaaagc
     8581 ccagctaaga gccagtactg ccgtaaacaa ttttttacgt tttactgcgg tcttcgcggc
     8641 agaggccacg gctgaaatac ataacgtcag tggggaaaaa agaaacggcc tggtgatgat
     8701 ccttggcata atatgagaac tcttatcaaa ttgagaatga tcatgattat catttgagtt
     8761 atatatgaaa tacaaccgtc gattcaatac gcaaggcgtt acaagcgtat ttatttttga
     8821 tttttatcaa gatgcttcgt cagctaacgt tcttgtttcc gactaaattt catcgcggga
     8881 aaaagctgag aacccagaga tatcgaggct tgcagagcaa agagaagaaa aaattatcac
     8941 tgttaacgac aaaatttagt gatgaggcat ggggggataa ccataaaaac aaggtggttt
     9001 acagggcgca ggataggttt cataaccccc tgtttattgt gttaatggtg gttaaaaaaa
     9061 aacttgatca taaaaaattg atcgataata attctcatta tcacgtttgc atttaacaaa
     9121 acacgaccat atggataaca aaaccatagc atttgcaagg gataacgcac agctctcttc
     9181 actcctgatg gggcatatcg ttttgcctga caaagagggc gtgcttttgc gtaattgccg
     9241 agttatcagg caatttcatg ggatataaac gcttgtaaac tcacccggcg aatattatcg
     9301 ctctgaattt taaataaatt tatttttcgc cagccacccc ggtattaacc caggcagcca
     9361 gcatttttta ttttcctcat ggagaatcgc tatgtcacgt ccgcaattta cctcgttgcg
     9421 tttgagttta ttggccttag ctgtttctgc caccttgcca acgtttgctt ttgctactga
     9481 aaccatgacc gttacggcaa cggggaatgc ccgtagttcc ttcgaagcgc ctatgatggt
     9541 cagcgtcatc gacacttccg ctcctgaaaa tcaaacggct acttcagcca ccgatctgct
     9601 gcgtcatgtt cctggaatta ctctggatgg taccggacga accaacggtc aggatgtaaa
     9661 tatgcgtggc tatgatcatc gcggcgtgct ggttcttgtc gatggtgttc gtcagggaac
     9721 ggataccgga cacctgaatg gcacttttct cgatccggcg ctgatcaagc gtgttgagat
     9781 tgttcgtgga ccttcagcat tactgtatgg cagtggcgcg ctgggtggag tgatctccta
     9841 cgatacggtc gatgcaaaag atttattgca ggaaggacaa agcagtggtt ttcgtgtctt
     9901 tggtactggc ggcacggggg accatagcct gggattaggc gcgagcgcgt ttgggcgaac
     9961 tgaaaatctg gatggtattg tggcctggtc cagtcgcgat cggggtgatt tacgccagag
    10021 caatggtgaa accgcgccga atgacgagtc cattaataac atgctggcga aagggacctg
    10081 gcaaattgat tcagcccagt ctctgagcgg tttagtgcgt tactacaaca acgacgcgcg
    10141 tgaaccaaaa aatccgcaga ccgttgaggc ttctgaaagc agcaacccga tggttgatcg
    10201 ttcaacaatt caacgcgatg cgcagctttc ttataaactc gccccgcagg gcaacgactg
    10261 gttaaatgca gatgcaaaaa tttattggtc ggaagtccgt attaatgcgc aaaacacggg
    10321 gagttccggc gagtatcgtg aacagataac aaaaggagcc aggctggaga accgttccac
    10381 tctctttgcc gacagtttcg cttctcactt actgacatat ggcggtgagt attatcgtca
    10441 ggaacaacat ccgggcggcg cgacgacggg cttcccgcaa gcaaaaatcg attttagctc
    10501 cggctggcta caggatgaga tcaccttacg cgatctgccg attaccctgc ttggcggaac
    10561 ccgctatgac agttatcgcg gtagcagtga cggttacaaa gatgttgatg ccgacaaatg
    10621 gtcatctcgt gcggggatga ctatcaatcc gactaactgg ctgatgttat ttggctcata
    10681 tgcccaggca ttccgcgccc cgacgatggg cgaaatgtat aacgattcta agcacttctc
    10741 gattggtcgc ttctatacca actattgggt gccaaacccg aacttacgtc cggaaactaa
    10801 cgaaactcag gagtacggtt ttgggctgcg ttttgatgac ctgatgttgt ccaatgatgc
    10861 tctggaattt aaagccagct actttgatac caaagcgaag gattacatct ccacgaccgt
    10921 cgatttcgcg gcggcgacga ctatgtcgta taacgtcccg aacgccaaaa tctggggctg
    10981 ggatgtgatg acgaaatata ccactgatct gtttagcctt gatgtggcct ataaccgtac
    11041 ccgcggcaaa gacaccgata ccggcgaata catctccagc attaacccgg atactgttac
    11101 cagcactctg aatattccga tcgctcacag tggcttctct gttgggtggg ttggtacgtt
    11161 tgccgatcgc tcaacacata tcagcagcag ttacagcaaa caaccaggct atggcgtgaa
    11221 tgatttctac gtcagttatc aaggacaaca ggcgctcaaa ggtatgacca ctactttggt
    11281 gttgggtaac gctttcgaca aagagtactg gtcgccgcaa ggcatcccac aggatggtcg
    11341 taacggaaaa attttcgtga gttatcaatg gtaatcatct gccccgatat ttcggggcat
    11401 ttatctggaa ggaagagaga caatgaacca ctacacacgc tggcttgagt taaaagaaca
    11461 aaatcccgga aagtacgcgc gtgacatcgc agggttaatg aatattagag aagcagaact
    11521 ggcatttgca cgcgtcacgc acgatgcgtg gcggatgcac ggcgatatcc gtgaaattct
    11581 ggcggcgctc gaaagtgttg gcgaaaccaa atgtatttgt cgtaatgaat atgcagtcca
    11641 tgagcaagtt ggtacgttca caaaccagca tttgaacgga catgccggat tgatcctcaa
    11701 tccgcgcgcg ctggatttac gtctgtttct caatcaatgg gccagtgttt tccacatcaa
    11761 agaaaacacg gctcgtggcg aacgccagag tattcagttc tttgatcatc agggcgatgc
    11821 attactaaaa gtttatgcca ccgacaatac cgatatggcg gcatggagtg agcttctggc
    11881 acggtttatc accgatgaga atacgccgct tgagttaaaa gccgttgatg cgccagttgt
    11941 tcaaacgcga gccgatgcca ctgtggtcga gcaagagtgg cgggcgatga ccgacgttca
    12001 tcagtttttt acgttgctca agcgccacaa cctgacgcgc caacaggcgt tcaatctggt
    12061 ggcagacgat ttggcctgca aagtatccaa cagtgcgttg gcgcaaattc ttgaatctgc
    12121 acagcaggat ggtaatgaaa tcatggtgtt tgttggcaac cgtggctgcg tacagatttt
    12181 caccggtgtg gtagaaaaag tggtgccaat gaaaggttgg ctgaatattt tcaacccgac
    12241 gtttactctt catctattag aagagagcat tgctgaagcc tgggttaccc gtaaaccgac
    12301 cagcgatggc tacgtaacca gtctggaatt gtttgcccat gatggtacgc agatagcgca
    12361 actttatggt caacgtacag aaggcgaaca ggagcaagcg caatggcgta agcaaattgc
    12421 ttcgctgata ccggaaggcg ttgctgcata acgtataatt aacgggaggt ctggctattt
    12481 attgctgggc ctctttatat tgatcaatcg ttcacaccag ataatcaata tgctgatggc
    12541 gtaccagctc attgatccgc ttaagattga ggatcatcat gatattgtgt ttatggcagt
    12601 agaacgtttt tagcgatatt ttcagtttct ccagaatatc ttccgttgtc atttgattcg
    12661 caatgcagct cagaacctga tgttgcttgc gcgaaagtga cacattcgct tcaggcacag
    12721 agcagtaact gccattgatg agtgcgacaa aatcaatgat agtgatagtc cgcggaacaa
    12781 aggtcaccgg cactttcccc aggcagcgtt taatattaaa gggggagaaa ataatgacat
    12841 ggacaggttt taatcgttca atttgagtga gaaaattaga atgaaataca ttattcatta
    12901 atgtatcaat aatgacgaag gcattctgat gcaacattac gtcgatctct tcttcgttct
    12961 gtatatgaac gacattacct ttactcagaa tgtttttcat cgcggtgaag aacatcgtat
    13021 ccctggtaat tataagaaac ataaccgact cctggtgcgg acattaactt tataatcagt
    13081 ttgccaaagg taattaacag gacagggctt ataataataa tagttagctt actgtgtatt
    13141 aatttgatgt aatgcatgat gcagatgctg agaaagcatc tgcatctttc ggtggtgtta
    13201 tttgaccagc tcaggtgtta cctgactcac cgcattggtg tagtaaggcg caccccagcc
    13261 cggttccggc cagaatgcac catagccgta atcccacagg ttataggtgg tattaaccac
    13321 ttctctcaaa tgccacacct ggatgccctg catattcact tccaggaagt tatacgggac
    13381 tttattgata aagccaggtt gttcaccctg aatggtgccg aggatggtaa caaagtgatt
    13441 acgatagttc actggatcaa ggaagccgct ttgtctggcg agcagtcggc cctgatagtt
    13501 ggcttcaata tcaggcttcg catagctatc caacggtaat acagcgattt ctaacaacgt
    13561 atccgttttg ccattgataa cgttgataac cttcccacca aagcgcgctt gttgaccaac
    13621 atataacccc ggctggttat gaacagcaac aaaacttttt tgaatatcag gttggttatt
    13681 tcctttgata ttttgcggaa ttgaactaca tgctgcaagc aaaaatgaaa ggctgagtat
    13741 gagtgcacct tttgtcatgt tcatgttact atccttatca acaaatatta aaaccataat
    13801 agatatttct ttatagtttt catctgattc tgaggcgata tgcctgatag taacgtaaac
    13861 ctgtctgatg tttatatccc ttctgtttag ttcaattccg ttattttggg gcgtggcatg
    13921 agtatccatc gccccataat ataacccttt gttttaaaat tacataaaat ctatttttat
    13981 tcaatgatta taaagtgcta ataatcaata ttatggtatt atattctgat aatgatttga
    14041 taagcgaatt cttttgccag atggtatttc tatttccacc ccataattta tatctttttc
    14101 attaggaagt ggcctcttta aataaggcgt gcgtgacatt ttttggggtt aagcgtctcg
    14161 tattgaaata cttcgaattg atattcagac atttctgccc atgtttgaat tttattgtat
    14221 cgagagagtg tcgtaaagga tttaacttgc ttataacatt ctttttggaa ttcttgctga
    14281 tatttcaata tgtcgttgta tgaaagattg gttatcctgg cctctaaaaa tttaattatt
    14341 gtcgaagcaa tttccgcttt tgctcgtaaa aagttgtcat taatggttaa taatgttgaa
    14401 aataaacctt cgatttgttt gtcaagatat tgttcgtctt tatataattt tggagtaggg
    14461 tgttctttat gtgaaaaaat agtgtcacct aaatttgcgt cagtgaaatt tatattaagt
    14521 agatcttcga aataaaacct tgacaaggtt agaacgaacc cggacaggtt ctgtcctgtc
    14581 atattgaaat caataaagtt ggttccggca aagactttca tcctgttttt attttcactg
    14641 tgctcaatat ataacttcgt cattttttga tacaggcttt gaatatcttt tatattagtc
    14701 ggtaagtcag ctcgttgttc agcaataaaa ttagtatctg aaaacatggt ttggacactg
    14761 aatcttactg gtattccctc ttctacgcta attttagtta tgattgcctt gtcgggttta
    14821 ggattagcga caacaatgat tcgacattca gcacttttgt tcttttcgta tctaatgtta
    14881 ttaatttgat taagtatttc ctgtgcctgc cgcgtaagtg gtgtcgaact tatcgattca
    14941 atgttagtaa taagacttac aagttgagcc acaacttttg agtagtctgc atctgtatat
    15001 ttggtgttcg tgcggcacgc ataaatttgt gggaggtgtt gccgcgctaa atcatattgt
    15061 tcagggttgt atccttctcc agagaatgtg gaattaatgg gttgttgttc ttcccgatta
    15121 tcacaaggcc acatcggtgt gtctggtgtt ttaacattgt cggtatctgt tgtgcggtta
    15181 taaatatcgc cgtgatatgt atttatcata cctggggtaa agtcaattga cataacattc
    15241 cttattcatt atctgtttta tgctaagatt tttaaattgg ataattaaag caggaatgga
    15301 ttttcttcta cgcacaataa atatcatgcg tcatatcttc ctgatataat tacctttgac
    15361 ttccctttta tctccacccg gtaatattcg tgtataaaaa agggcattga tagtatcgta
    15421 aaggaaaacg ttaacaacta tcaaaaagcg ctcattttaa aaagttatga tgttctccga
    15481 tagtgtgtga aatgatattt ttgtgatgtt ttagatgtgt aataataaaa tgggttaagg
    15541 tatcttataa ttattgaata atttgttgtg taaagcactg ataaatcata atccagtacg
    15601 tttttaaact gctgcactgt atcgctgatc actaagcagc tcactgatac atcatgctgg
    15661 acgcgataat tggaatctca cgcagggagt aatgtgtaaa tagcaaaatc gcctgatgcg
    15721 ctacgcctgt caggcctaca aataacatac aatttattga attttcatga ctttgtagat
    15781 cggataagac gcgttagcgt cgcatccgac ataaataaag cgcacttttc taacaacctg
    15841 tgggggggat atagccgctt tatttcaggc gcttacccgc ttcatcaacc actttctcgc
    15901 catcttcctt ggtgaatgcg cctttttgcg catctggcag aatttccagc accacttctg
    15961 aagggcggca caggcgagtt cccagcgtcg tcaccacaat cgggcgatta atcagaatcg
    16021 ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa tttatcttcc gcaaggccca
    16081 gctcctcata cggttcgacg tttttacgca gcagcgcgcg tacggaaatc cccatatcgg
    16141 caatgagttt gaccagttca tcgcgcgttg gcggagtttc cagataatgg ataatggtcg
    16201 gttctgtgcc gctgttgcgg atcatctcca gcgtattacg cgacgtgccg caggccgggt
    16261 tgtgataaat ggtaatgttg ctcatatcag tatctcatta caaagtgaaa gagagacgta
    16321 gcgccagcgc agccagcgtc acaaacagca caggcagggt catgataatc cctgtacgga
    16381 agtaatatcc ccagctgata gtcatattct tctgcgaaag tacgtgcagc cagagcagcg
    16441 ttgccaggct accaatcggg gtgattttcg gccccaaatc gcagccaatc acgttggcat
    16501 aaaccatcgc ttctttgata acgccagatg ccgtgctgcc atcaatggac aacgcgccaa
    16561 ccagtaccgt cggcatattg ttcataatag aagagaggaa ggcggtgagg aatccggtgc
    16621 cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt gagtacgcca gaaagatatt
    16681 ccgttaatcc ggcattgcgc aggccataaa ccaccagata catgccgagc gagaagatga
    16741 caatctgcca gggcgcaccg cgcagcactt tacccgtatt aatcgcatga ccgcgtttag
    16801 cgacgacaaa taatatcaac gcgcccactg ccgcaatggc gctgaccgga atgccgagcg
    16861 gttccaggac gaaaaatccc accagcagaa gcagtaaaac aacccagcca gttttgaacg
    16921 tagcaggatc tttaatcgct tctgcgggag atttcagcag cgccatgtcg tagttctgcg
    16981 gaatatcttt gcggaaatag agatgcaaca tcaccagcgt ggcaacaatc gcggcgatat
    17041 ccaccggcac catcaccgag gcatattcgc gaaagccgag gccaaagaaa tctgcggaaa
    17101 cgatattcac caggttggag acaataagcg gcaggctggc ggtatcggca atgaatccgg
    17161 ccgccatcac aaacgccagc gtagtgcctt tactgaaccc taaagccagc agcatggcga
    17221 tgacaatcgg tgtcaaaata agcgccgcgc catcattggc aaacagggcg gcaacggcag
    17281 caccgagcag gacaatccag gtaaacagca agcgaccgcg tccattgccc cagcgtgaaa
    17341 cgtgcagtgc cgcccattca aaaaagccgg actcatccag cagcaggctg atgataataa
    17401 cggcaataaa cgcagccgtc gcgttccaga cgatattcca caccaccgga atatcgcccg
    17461 gatgtactac gcccgtaact aacgccagta ctgcgccgag cgttgcactc cagccgatgc
    17521 ctaaaccttt cggctgccag ataaccaata caatggtcag gacaaagata gcgcctgcca
    17581 gtaacataat gcctcccgga aaaacacatg tgcaaatcca tatgtgttta gataaatttt
    17641 ttaactacaa gtgttcttac tgtctacgga acaattttgt ctggccaggt tgcggacaat
    17701 cacctgaacc ttttcctgtt cgcatcgcca ggcctgctca ataattttcg cggcccatga
    17761 tggaatatgc ggtgataagc ggtaatgaac ccacttaccc tgcttgcgat ccagcaacaa
    17821 accgctttca cgaagaagcg ccagatggcg ggagatcttg ggctgcgact ggtcgagagc
    17881 agtgcagaga tcacagacgc ataactctcc cagctcgctg agcagtaaaa cgatgcccag
    17941 acgggtttca tcggcaagaa ttttgaacag ttggatgggt aacagaaatg acatattgcg
    18001 ctcctgattg ttgcaggtag tgtctctctt cgaagcggat aagtcaaaaa catatatggt
    18061 ttaacgaatg tgtatgtgca gaggaaggta ataggtgtga attaagcgtt ggctattcat
    18121 ttgaaaggag gtctgaatca ggacgtaaaa aaaccgactt tgcgtcggtt ttttactttc
    18181 cagcccagag ttggtggctc tggctggagt gagaatgccc acatctaacg gttcaatact
    18241 attagccagt ggctaacatt gcaagcgtta gcgatttctt cagctggaga agggacagtt
    18301 tgctatacat gttggtgaaa gaggatttat atactttcat cgggtcgctg ccgtaccagg
    18361 ctttgtccga atccggatgt aaaaaaaccg acttcgcgtc ggttttttta ctcttcagcc
    18421 ctgagttggt ggctctggct ggagtgagaa tgccatcatc taacgcatca atactaatac
    18481 catactgaaa ctattgcaag gacatgctgg ttttataacc tgaatatact gtatgattat
    18541 ccagttagct ctgaggcatt ttcactctgg caatgcgcat aaacgctttc aaagtcctgt
    18601 tcagtggtac gggtggtgcc gttaactgat gctctggccg gagtgagaat accattatct
    18661 aacaataggg catgcgccgt gacaggcgat ggatgagtaa gcggatgcat tctcactcca
    18721 tcgtatggag aacacgggtg attgataaag caatcatcgt tcttggggcg ttaattgcgc
    18781 tgctggaact gatccgcttt ctgcttcagc ttctgaactg atagcggaaa cgtaattaag
    18841 ggcaaagagc acaccactct ttgcccttta acatttaacg catggtcacg aactcttctg
    18901 ccgccgttgg gtgaatggcg acggtattat cgaagtcttt tttggttgcg cccatcttca
    18961 gtgccaccgc gaagccctgc aacatttcgt ccataccaaa accaatgccg tgaataccga
    19021 caatcttctc ttctggccca acgcacacca gcttcatgcg gcacggctgg cggtgagtgg
    19081 tgacggcggt atacatcgcg gtgaaagagg atttatacac tttcacctga tcgtcgccgt
    19141 actgctcgcg cgcctgcggt tccgttaaac caacagtacc aatcggcgga tggctgaaga
    19201 ccacggtcgg aatgttgctg taatccagat gctcatccgg cttgttatta aacaggcgtt
    19261 cagagagacg gcgacccgct gcaactgcca ccggtgtcag ttctactgca cccgtgttat
    19321 cgcccaccgc gtaaatacct tcaacgttgg tgttttgata tttatcgacg acgatatagc
    19381 ctttttcgtt ggttttaacg ccagcggctt cgaggttgat gttgtcattg gcaggctcgc
    19441 ggccaatcgc ccaaatcagg caatccaccg tttcactgcg accatcttcc agctccagcg
    19501 tcaggctacc atcggcattt ttcactaccg ctttcgggat ggcgttggtg tgcaactgcg
    19561 ggccttcggc gttcatcact tcgaccagcg tttcagagat catcgggtcg aagctgcgca
    19621 gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc gaggccgtta atcacgccag
    19681 ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac acgctctggc aaagcaggaa
    19741 gggcaaagaa accatcagaa tcaataccgt attccacgcc cggaatatcc gggtggctcg
    19801 gacgaccgcc ggtggcgatc agaatatgat cggctgtgat ggtttcaccg ttaacttcca
    19861 gcgttttggc atcaacgaag cgggcaaagc ctttgattac atcaacgtta tttttaccga
    19921 gcacgttttc ataggaagta tgaatacggt cgatataggc ggtacggctg gcgatcaacg
    19981 tttcccagtt gaatttattg atagtggtat caaaaccata atccgggccg tacatatgga
    20041 tcgcttcacg gatttgcgcc gcgtgccaca tcactttctt cggtacgcag ccgacgttca
    20101 cgcaggtgcc gcccagctct ttggcttcaa tcagcgcgca tttctgaccg tacatagccg
    20161 cgcggttgat ggaggcgata ccgccgctgc cgccgccgat ggcgatgtaa tcatagtgtt
    20221 tagtcatgac agagtgtcct tatcgttgat taccgcgatt gtagcgcgag acgtaatagg
    20281 tgccagcaat ggctgcaatt actctggcac gatccagctt acggtggcgt gcccggtgcc
    20341 agctggaacc agtttgctgt gcagccacgg cagcacgtta ttcatctgct gttccagttt
    20401 ccacggcggg ttaatcacaa tcatgccgga agcggtcatg ccacggcgat cgctgtctgg
    20461 cagtaccgcc agttcaattt gcagaatttt gcgaatgccg gtcgcttcca ggtcgtggat
    20521 catgcgctta atttgctggc gcagcaccac cggataccac aatgcgtaag tgccagtggc
    20581 gaaacgtttg taaccttctg ctatcccgct gactaccgcc tgatagtcgg ttttcatttc
    20641 atacggcgga tcgataagga ttaaaccacg gcgagaaacc ggcggcagtt tggccttaag
    20701 ctgctggaaa ccgtcggatt tttcgacgcg cgcacggctg tctttctgaa attcagaacg
    20761 cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc aggctgtcct gttcacgcag
    20821 taactgacgg gcaatcaacg gtgaaccggg gtagtaacgc aactgaccgc tacggttgaa
    20881 gtgttttacc atattgatgt acggctccag ctccgcaggc aaatcgtcct gctgccagat
    20941 acgggcgata ccttcgagat attcgccggt acgctcggta tgttcgctgc ctaactgata
    21001 acgcccggcc cctgcgtggg tgtcgagata gagaaacggt ttatctttct ctttcagcga
    21061 ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg gcgtggttgc cagcgtgaaa
    21121 gctgtggcga taactgagca tgggtaaagg tgttccggta agtaaaaatc ggcccgtatt
    21181 cggggcgcac aaaagcctat caggacagta taccgaaaag aggccgccgc cgcgaaagcg
    21241 taacgtttct cattgaaatt cactacactt aaccccatgc tacaaacatt atgtaaagcg
    21301 cctgttgagc gcttccttaa cctctttaac caggactgcg ctaatgacga atccgttact
    21361 gactcccttt gaattgcctc cgttttctaa aattctcccg gaacatgtcg ttccagccgt
    21421 gactaaggca ttgaatgact gccgcgaaaa tgtggagcgc gtagtagcgc aaggagcacc
    21481 gtacacctgg gaaaatctct gccagccgtt ggcggaagtg gacgatgtgt tggggcgtat
    21541 tttctccccg gtcagccacc tgaactcggt gaaaaatagc ccggaattgc gcgaagcgta
    21601 cgaacaaact ctgccgctgc tgtcggaata cagcacctgg gtagggcaac atgaagggct
    21661 gtataaagcg taccgcgacc tgcgcgatgg cgatcattac gccacgctga acacggctca
    21721 gaaaaaagcg gttgataacg cactgcgcga ttttgaactc tcaggcatcg gtctgccgaa
    21781 agagaaacaa cagcgttacg gcgaaattgc cactcgtctt tctgaactgg gcaaccagta
    21841 cagcaacaac gtccttgatg cgacgatggg ctggactaaa ctcgttaccg acgaagcgga
    21901 actggcgggg atgccagaaa gcgcgctggc tgcggcaaaa gcccaggccg aagcgaaaga
    21961 gctggaaggc tacctgttga cgctggatat cccaagctac ttgccggtaa tgacctactg
    22021 cgacaaccag gtcttgcgtg aagagatgta tcgtgcttac agcacccgcg cttcagatca
    22081 aggtccaaac gccggtaaat gggataacag taaggtgatg gaagagatcc tcgcgctgcg
    22141 tcacgaactg gcgcaactgc tgggctttga aaactacgcg tttaaatcac tggctaccaa
    22201 aatggcggaa aacccgcagc aggtgcttga tttcttaacc gatctggcaa aacgcgcgcg
    22261 tccgcagggc gaaaaagagc tggcgcaact gcgtgccttc gccaaagccg aatttggcgt
    22321 ggatgagttg caaccgtggg atatcgcata ctacagcgaa aaacaaaaac agcacctcta
    22381 cagcatcagc gacgaacaac tgcgtccgta cttcccggaa aacaaagcgg ttaacggctt
    22441 gtttgaagtg gtaaaacgta tttacggcat caccgctaaa gagcgtaaag atgttgatgt
    22501 ctggcacccg gatgtacgtt tcttcgaact gtatgacgag aacaacgaac tgcgcggcag
    22561 tttctacctc gacctgtatg cccgtgaaaa caagcgcggc ggggcgtgga tggatgactg
    22621 cgtaggccag atgcgtaaag ccgatggttc gctgcaaaaa ccggtcgcgt atctgacctg
    22681 taacttcaac cgcccggtga atggtaaacc ggcgctgttc actcacgacg aagtgatcac
    22741 tctgttccac gagttcggtc acggcctgca ccatatgctg acccgcatcg aaaccgccgg
    22801 tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa ctgccgagtc agtttatgga
    22861 aaactggtgc tgggagccgg aggcgctggc gtttatctct ggtcactacg aaaccggcga
    22921 accgctgccg aaagagttgc tggataaaat gctggcggcg aagaactacc aggcggcgct
    22981 gtttattctg cgccagctgg agttcggcct gttcgatttc cgcctccatg ccgagttccg
    23041 cccggatcag ggagcgaaaa tcctcgaaac tctggcagaa atcaagaaac tggttgccgt
    23101 agtaccgtct ccatcctggg gccgtttccc gcacgctttc agccatattt tcgccggtgg
    23161 ttatgccgca ggttactaca gctacctgtg ggctgacgta ctggcggcag atgctttctc
    23221 gcgctttgag gaagagggca ttttcaaccg tgaaaccggg cagtcgttcc tcgacaacat
    23281 tctgagccgt ggcggttcag aagagccgat ggatctgttc aaacgcttcc gtggtcgtga
    23341 accgcagctg gatgcgatgc tggagcatta cggcattaag ggctgatcat tcagtgaaaa
    23401 tctgcttaat tgatgaaaca ggcaccggag acggtgcctt atctgttctg gcggcccgct
    23461 gggggctgga gcacgatgaa gacaacctga tggcgctggt gttaacgccg gaacatctgg
    23521 aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt tgttgatttt gttggcggag
    23581 cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga ggcggtggcg aaagcggtgg
    23641 gcattaaagg cgattatttg ccggatgtgg tggacgctac cgcagggctg gggcgcgatg
    23701 cctttgtgct ggcttcggtc ggctgccgcg tgcggatgct ggagcgtaat ccagtcgttg
    23761 ccgcgctact cgacgacggc ctggcgcgtg gatatgcgga tgcggaaatc ggcggctggt
    23821 tgcaggagcg tttgcagtta attcacgcct ccagcctgac ggcgctgacc gatattaccc
    23881 cgcgtccgca ggtggtctat ctcgacccga tgttcccgca taagcagaaa agcgcgctgg
    23941 tgaaaaaaga gatgcgcgtc tttcagtcgc tggtgggacc ggatcttgat gccgatggat
    24001 tactggagcc tgcacgcttg ctggcgacca aacgcgtggt ggtaaagcgc ccggactacg
    24061 cgccaccgct agcgaatgtc gccacgccaa acgcggttgt gactaaaggg catcgctttg
    24121 atatctatgc aggtacgcca gtgtaaaagt aaagcagggt agcgataacg ctaccctgct
    24181 ttctgcaaga ataattaatg gctttccggc gtcgcaatca tgcgtttcag ccacggcacc
    24241 atcaacagca tcactactgc aacgcccagc gtgaccagac caatcttacc aaacacgttg
    24301 gtatagacag gcaacgtctc aagcggatcg gtaatgttgt ctggtactgc ggtaaatgtt
    24361 gccacatagc cgcccagcaa gaacgcggca gcctgtgtca ggaaccacat cccgagaata
    24421 aagcccatca aatgctgcgg caccagagca gcaatcatcg ccaggccaag ggcgctaata
    24481 aacagttcac ctaagctctg gaataagtac accagcacga taaaccatgg cgatgtcagc
    24541 ccttgtgcat ccgcaaacca cattcccgca gctgccgccg tcaaaaagcc cagtgagcac
    24601 ataaacatgc cgagagtaaa tttcatcggc atcgagaggt ctttgccttt gttacccaga
    24661 tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca cccagaacgg gttaagcgcc
    24721 tggaagctga ccgggttgat ggaaaaaccg agaatttcat gatgcacgtt gttgatggca
    24781 aagaagttca gcgatgttgg catctgggcg tagagaatgt aaaacaccac cgcttcgagc
    24841 atcaggacaa aggcgacaaa cattttattg cgcccggttt tatccagctt gaatgcctga
    24901 cgaaagaaga tgatggtgac gacgatggag agaacaatca gcaccagatt ggcgacttct
    24961 acgttgtgca tcagccaggc gcagacgaag atcatcacca cgctgccaag taatacgtac
    25021 aacagtttgc tgaagctcat tggccggaag tcgggctcag aaccaatgtc tttcaccatt
    25081 ccacgacagg cgatgtaaac cagtaatgcg ataattaacc ccgcaccgca caggttgtag
    25141 gtgactgaat agccgaattt atcagcgatc acaggggcca gcgataacgc tatcaacgag
    25201 ccgatattga tcgacatata gaacagggtg aatgtgccat caaggcgcgg atctttcggc
    25261 ggatagcact tcgaaagcaa gctggctggg ttagctttaa acaggccgtt accgacagcg
    25321 atagtcccca gggcgatgaa aatcaggtca ggcttaagta gcgacatgcc ggtcatgaag
    25381 tagccaatcg ccagcacaag tgctccaaga acgatggtgc gtttggtccc cagcaggtgg
    25441 tcgccgacat agccgccaat ggaaatgagg ccatagacca gtgcagcaaa agcaccaaaa
    25501 gtgacaaaag cctgctcttg cgagaatcca agctgtttaa cgaagaaaac cgccagtacg
    25561 ccctgcacgc cgtagtagcc gaatcgctcc cataactcga caaaaaagat catgaaaaat
    25621 gggcgaggtt gctgcagcat ccccatgggt gttgttgtat tcatatttat taaccttcca
    25681 ataccatcct gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt tacatctgtc
    25741 agagattctt aatattgatg cggtgaatag attaatacac tattacaaca gaaaataacc
    25801 agctgatcac actaatgatg gaaaatatga tggtgttaaa tattgtattg gctggggatt
    25861 ttgattgcga acgcagaccg taggccagat aaggcgttta cgctgatcag gtaacaaggc
    25921 gcgacaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg aacacgtcgt ggagggaaga
    25981 ttattcttct tcgtcgcgca gcggaacaat cagcatatca acgtgaacgg tgttgatcag
    26041 ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg tggtgaccac aaaccactag
    26101 atccatatcg tatttcttga ttgcatcgac cagaacctgg cccaggtcgc cgctgccgct
    26161 cagggtttca gtgattgggt agcctgcatt agtggaaagc tcggtcagcg catgatgtgt
    26221 ctcttcagag atgcgtttct gcatatcacc aagattcaca tcaataagcc cggtatatag
    26281 gtcagagtag tttacatcta cgtggatcag agaaactttc gcattgtatg ggcgagccat
    26341 agagactgct ttctctacca gaactttgct ttccggggag agatcgaccg cgatgagaat
    26401 gtgtttataa gccatagtgt tactccttcc ataaagttgt cgatgactgg ccagctagcg
    26461 tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg tgcgcggggc taacccagcc
    26521 gtaaagattc agtgttaaga ccatccttac cttatagcga cccggatgat ccgtcaatcc
    26581 gccttgctta cccgttagat aaacaaattt tcgacatgaa tatattgata gtggttaacc
    26641 ttctggaaaa aaaacaacct gatctcctac actatctata gagccgctcg gatgttggtc
    26701 accacaaaag cggtttctcc aggacctggt gttaactgat tggcagcgta tcgggagcgg
    26761 tagccccggg gtggaactcc gcgggcaggt cgccggggag gaggtatgat aagcaccgtc
    26821 gcattatttt gggctttatg tgtcgtttgc attgttaaca tggcgcgcta tttctcatca
    26881 ctacgcgcgt tgttagtggt actgcgtaac tgcgatccat tgctctatca atatgttgat
    26941 ggagggggct tttttacctc acatggccaa cccaacaaac aggtgcgtct cgtttggtat
    27001 atctatgccc aacgttatcg cgatcatcac gatgatgagt ttattcgccg ctgtgagcgg
    27061 gtgcgtcggc agtttattct gaccagcgca ttgtgtggtc tggtggtggt cagcctgatt
    27121 gccttgatga tttggcatta agcacttcag caataataac gcataaaaaa agcgggccag
    27181 taaactgacc cgctcgtttt ggtgcgtact attacaggaa ctgcaaggag agccagtaaa
    27241 gcccgccgga aagcagtacc gcagccggaa gggtaaacac ccaggccatc agaatgctgg
    27301 tcacggtttt acgctgtaag ccgccaccat ctaccaccat cgtccccgcg acagaagagg
    27361 agagtacgtg agtagtggaa accggcatcc cggtataact cgccaggccg atagacactg
    27421 ccgcagtcat ctgggcagac atcccctgag cgtaggtcat gcctttctta ccgattttct
    27481 caccgatagt cgttgccaca cgacgccagc caatcatcgt accgatacct aacgccagcg
    27541 cgaccgccat gatgatccac accggtgcat actcgatggt gctaagcatg tcggacttca
    27601 gtttcttcaa caggcgctga tcgtcagcac tcacgccagg catcttcacc actttgtcga
    27661 tagtgtcaga aacgcacagc ataatgcggc gcatctggct acgttgatca agcgacagct
    27721 tgtcgtagct ttccacatcg gtggtcaaca tacctttcag gcggttgagc gcgttaatgg
    27781 tattcgacgg atggcagtgg aactccgcag gttgcgttgc gccagcttcc ggagccggta
    27841 ctaactgatc agcaccagta gcttgtttca gcagcgcagg atgctgctca aagtaagctt
    27901 cgacgttgtt gatggcatca cgggtacggg tgatttcgta gccagtggca ttcatgttca
    27961 ccacgaagcc tgctggtgcg acaccaatca ataccaacat aaccagacca atgcctttct
    28021 gaccatcgtt tgcgccgtgc gaaaacgcca cgccgatagc ggaaaggatc agcgcgatac
    28081 gcgtccagaa cggcggcttt ttcttgccgt ctttcttttc acgctccgct ggggtcaggt
    28141 ggatacgggc gcgtttcttg gtgccgctcc agtagcgacg cagcaagaaa atcagaccgc
    28201 cagcaaacac caggccgaca ataggggaaa cgatcagaga accgaaaata cttaatactt
    28261 tcgggatatt gagtgcatcc accactgacg tcccggtcat caacgcattg gttaaaccaa
    28321 tcccgatgat cgcgccaatc agcgtatgag agctggatgc aggcaaacca aagtaccagg
    28381 tacccaggtt ccagataatc gccgccagca acatagagaa caccatggca aggccatgag
    28441 acgatcccat attaagcagc agatccgtcg gcagcatatg cacaatggca taggcaacac
    28501 tcagaccacc cagcaaaaca cccaaaaagt tgaacaccgc tgccataacc acggcgagct
    28561 gagaacgcat cgcgcgggta tagataacgg ttgccacggc gttggctgtg tcatggaaac
    28621 cattgatggc ttcgtagaac agcacaaaag ccagtgcaag caataataac agcccggtat
    28681 gcaaatccag gccagcaaac aaatgtagca taggacgtta cgccattttg aggacatgaa
    28741 cgcggcgcat tatcagtgac tttcgcggcg cgggcaaagt gaaatataga ctttttttga
    28801 tttgcctcct gtatggattt cactcaaaaa ataattatct tatataattc aggcaaatac
    28861 ttccttttag taatattgat gctggtgcga ccactgagga atctttacaa ttcacgcccg
    28921 ttttttctaa gaggagcgca acgtggaaag gtttgatgcc attattatag gcgctggtgc
    28981 ggcgggtatg ttctgttctg cgctggcagg tcaggcagga cgccgggttc tgctgatcga
    29041 taatggtaaa aaaccagggc gcaaaatcct tatgtctggc ggtgggcgct gcaactttac
    29101 caacctttat gtcgaaccgg gcgcttatct gagccagaat ccgcattttt gtaagtctgc
    29161 actcgcgcgt tttacccagt gggatttcat tgatctggtc aataaacacg gcatcgcctg
    29221 gcacgagaaa acgttagggc aactcttctg cgatgactcc gcgcagcaga ttgtcgacat
    29281 gctggtggat gagtgcgaga agggcaatgt gaccgtcaga ttgcgtagcg aagtgctgag
    29341 tgtggcgaag gatgatacag gcttcacgct tgaactgaac ggcatgactg tcggttgtga
    29401 aaagctggtc atcgcgaccg gcgggctgtc aatgccgggc ctgggcgcgt cgccgtttgg
    29461 ttataagatt gccgaacaat ttggcctcaa cgtgctgccg acccgcgcgg gtctggtgcc
    29521 attcactctg cataaaccgt tgctcgaaga gttacaggtg ctggcgggcg ttgcggtgcc
    29581 ttccgtgatt accgctgaaa acggcaccgt tttccgtgag aacttactct tcacccatcg
    29641 cggcttgtct ggaccggcgg tgttgcaaat ttccagctac tggcaaccgg gggaatttgt
    29701 cagtatcaat ctgctaccgg atgtggacct cgaaaccttc ctgaatgagc agcgtaacgc
    29761 acatccgaat cagagcctga aaaacacact ggcggttcat ctaccgaagc ggttggttga
    29821 acgcttacag caactcgggc aaatcccgga tgtttcgcta aaacagctca acgtgcgtga
    29881 ccaacaggca ctgattagca cattgaccga ctggcgcgta caacccaacg gcactgaagg
    29941 ctatcgcact gccgaagtga cgctcggcgg cgtggacacc aacgaactct cttcacggac
    30001 gatggaagcg cgcaaagtgc ctgggctgta ctttatcggc gaagtgatgg acgttaccgg
    30061 ctggctgggg ggctataact tccagtgggc gtggtcgagt gcgtgggctt gtgcgcagga
    30121 tttgattgca gcgaagtcgt gctgagctat ttttcctaaa gatgaaaatt attagagtcg
    30181 cattaaaaat gagctaattt tgatagtggc tatcttgtga ttattttcta atgagcccgt
    30241 gaactgaacc ccccatgctt aatataaggt ggagggaaag gtgattgaaa actcacccag
    30301 tgggctgatc tttataaaat atgaacaggt tgaaaaaaaa cagtaacttt gctgtttttt
    30361 atacttaacc actatgcatt aatgctgcgt tatttcattc tacctaagaa aaaccagcgt
    30421 tacgcaaatg gttaacgctg gttttatccg gtacgttgca attatttttt agcagaaccc
    30481 gcttctaata ttgaaacgat tgagaacaac gtaaagcata ccgctcccaa accgaccaga
    30541 acccgcgaag gaataaaata tcccatatcg gtctgcgcca tttccgcaag aaacgatgct
    30601 aggaaaaggc aaaacaggca ggtgaagacg ggaatcatcg gtattctgtt ggctaacgaa
    30661 caggtacgtc tccagaccag tgccagtagc cagacttttg agaatatgct gtaacagatc
    30721 attcccaagc aaatgaggat aataccggga gccagtcggg cgcttgcatc agaactgact
    30781 aagacgtata tgccctgcag caccgtgatt gagccgagaa aaataaccca ataacaccac
    30841 agccagtgct ctttcgttga aaacgtatta cgtgtttgat ggacgattgt ggcaacaagg
    30901 ccaattagac aggcacagat tgcggttaac ccgagcaata cgtgaccggc gacataatgc
    30961 ggagttatgt cggcactacg taacagcgta atagaccaaa tgaaaccgag cagggtgagt
    31021 aacacgggaa cggcaatgag gcaattaccg attaatgaag aataagcctg taccggtgtt
    31081 ccgtcgctct tgctccccgc tgcgttttta ggaatgagca gaaagtgacc ggatgacgct
    31141 gccaccgtcg atacacaggc ggcaatcata ccaacgccga aaataacatg gccggcgaca
    31201 aactcgtctg ccatcacatc attacctgct aacagtgccc aaccccatat catggtgata
    31261 attgaccccg catagccaat aatggggaac aatgtattgt aaaacgtatt aacgccgcgc
    31321 gtgagctgcg aaatgataat aaatgcggta gtgaataatg ctaggcatat ggctgccaga
    31381 gaaatgagca catgacccgc gacgaaatac tcgctttgcc ctgaactttc cagcacaaag
    31441 ccacctaacg cgatacaaat caagcccata attaaaggga ttaatttgaa aagccacccg
    31501 atatatatgt tcactttaaa ctcctcaggt atccctgcta atattgaaat gtgcattaat
    31561 gataatttca gcctgttaat acaatgacga gatatcatta aagacaaatg catagcataa
    31621 ctatagcggg gtttactttg cagagcgcta cttttcagcg ttcaaaaggc catgataaat
    31681 aaatatttat cctaagatta attcttattc tgttgttttt tacaattatc ttccagggag
    31741 ggattctttt tcattatttg gtaaaagacc ggatggtctc ggtgcttaaa atcagcgagt
    31801 atatacgcat cattgcttta t
//
LOCUS       DAZFBR010000050        31534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFBR010000050 DAZFBR010000000
VERSION     DAZFBR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.050"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(263..4144)
                     /gene="entF"
                     /locus_tag="ACHJZ2_002494"
     CDS             complement(263..4144)
                     /gene="entF"
                     /locus_tag="ACHJZ2_002494"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO4568582.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQHYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(4141..4359)
                     /locus_tag="ACHJZ2_002495"
     CDS             complement(4141..4359)
                     /locus_tag="ACHJZ2_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HFO4568583.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4362..5564)
                     /gene="fes"
                     /locus_tag="ACHJZ2_002496"
     CDS             complement(4362..5564)
                     /gene="fes"
                     /locus_tag="ACHJZ2_002496"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO4568584.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5807..8047
                     /gene="fepA"
                     /locus_tag="ACHJZ2_002497"
     CDS             5807..8047
                     /gene="fepA"
                     /locus_tag="ACHJZ2_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO4568585.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            8222..8842
                     /gene="entD"
                     /locus_tag="ACHJZ2_002498"
     CDS             8222..8842
                     /gene="entD"
                     /locus_tag="ACHJZ2_002498"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO4568586.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8964..9116)
                     /gene="hokE"
                     /locus_tag="ACHJZ2_002499"
     CDS             complement(8964..9116)
                     /gene="hokE"
                     /locus_tag="ACHJZ2_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO4568587.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9467..9619)
                     /gene="hokE"
                     /locus_tag="ACHJZ2_002500"
     CDS             complement(9467..9619)
                     /gene="hokE"
                     /locus_tag="ACHJZ2_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO4568588.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            9588..9855
                     /locus_tag="ACHJZ2_002501"
                     /pseudo
     CDS             9588..9855
                     /locus_tag="ACHJZ2_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10058..11176
                     /locus_tag="ACHJZ2_002502"
     CDS             10058..11176
                     /locus_tag="ACHJZ2_002502"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO4568589.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            11242..11490
                     /locus_tag="ACHJZ2_002503"
     CDS             11242..11490
                     /locus_tag="ACHJZ2_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO4568590.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            11555..11923
                     /locus_tag="ACHJZ2_002504"
     CDS             11555..11923
                     /locus_tag="ACHJZ2_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO4568591.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            12017..12670
                     /gene="nfsB"
                     /locus_tag="ACHJZ2_002505"
     CDS             12017..12670
                     /gene="nfsB"
                     /locus_tag="ACHJZ2_002505"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO4568592.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12778..14025
                     /gene="ybdG"
                     /locus_tag="ACHJZ2_002506"
     CDS             12778..14025
                     /gene="ybdG"
                     /locus_tag="ACHJZ2_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO4568593.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(14093..15469)
                     /gene="pheP"
                     /locus_tag="ACHJZ2_002507"
     CDS             complement(14093..15469)
                     /gene="pheP"
                     /locus_tag="ACHJZ2_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO4568594.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15571..18708)
                     /gene="cusA"
                     /locus_tag="ACHJZ2_002508"
     CDS             complement(15571..18708)
                     /gene="cusA"
                     /locus_tag="ACHJZ2_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO4568595.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(18720..19943)
                     /gene="cusB"
                     /locus_tag="ACHJZ2_002509"
     CDS             complement(18720..19943)
                     /gene="cusB"
                     /locus_tag="ACHJZ2_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO4568596.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19959..20291)
                     /gene="cusF"
                     /locus_tag="ACHJZ2_002510"
     CDS             complement(19959..20291)
                     /gene="cusF"
                     /locus_tag="ACHJZ2_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO4568597.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20315..21697)
                     /gene="cusC"
                     /locus_tag="ACHJZ2_002511"
     CDS             complement(20315..21697)
                     /gene="cusC"
                     /locus_tag="ACHJZ2_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO4568598.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21854..22537
                     /gene="cusR"
                     /locus_tag="ACHJZ2_002512"
     CDS             21854..22537
                     /gene="cusR"
                     /locus_tag="ACHJZ2_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO4568599.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22527..23975
                     /gene="cusS"
                     /locus_tag="ACHJZ2_002513"
     CDS             22527..23975
                     /gene="cusS"
                     /locus_tag="ACHJZ2_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO4568600.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSNARGTRFVIVLPERG"
     gene            24569..26470
                     /locus_tag="ACHJZ2_002514"
     CDS             24569..26470
                     /locus_tag="ACHJZ2_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO4568601.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            26498..26959
                     /locus_tag="ACHJZ2_002515"
     CDS             26498..26959
                     /locus_tag="ACHJZ2_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO4568602.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            26979..>31534
                     /locus_tag="ACHJZ2_002516"
     CDS             26979..>31534
                     /locus_tag="ACHJZ2_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4568603.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIRWSDNDQT
                     WSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAP
                     DGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYCYDERGLL
                     SNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQDERRLGIEYD
                     HHNWPETLTDVMGHEHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAGTGAE
                     GFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVIN
                     PQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYSRDAAGRL
                     AEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDE
                     HTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVL
                     RHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGNVSGVTSR
                     EDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQSAGRHRISG
                     ETDQYAAGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSRDQLTGYRSA
                     QGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGF
                     ELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGL
                     ALSLPADTDYPDPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDP
                     LGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNSLGNRALKYA
                     NGDWIKSEAAFNKYINMINKRLELT"
BASE COUNT         7619 a         8532 c         8325 g         7058 t
ORIGIN      
        1 tcgggaaaat gagcgtcact tccctgttta atattcagtg atgacatggg gtataacctg
       61 aaaatgagtc caataggggg aaatctccac ggcgctcact ttaccagttg gtgagaatta
      121 tccgaagctg aagtttgtaa attcattcca ctgaatgcaa cattcactct atttaagaaa
      181 aataaccagc aaacttataa attaaataaa tgataaaccc ataattattt cctgcaatta
      241 tgggtttata gataatgtta atttatctgt ttagcgttgc gcgaataatc ggcccaattt
      301 tttcaaatgc ccctggagag ataatatcca catgcgcaca atcctgacga tagatatcca
      361 gctccgcaat ccacggcgac caggcgcgtt cggggctcat accttcctga agcgtacgtt
      421 cagcaacaaa cagcgtcgct tttccgtcaa acggtacgct atgagcagtc gtcagcaggc
      481 gcacagcatc agcgtagttg ccttcaatgg tggtaaacaa ctccgttgaa gtacttccct
      541 gctgtgccgc caggaaggcc tcgcgctcgc ggttaatctc cgccagcact tccgggtcca
      601 gaccattagc ttctttttcc tgccagtttt gcgtttctgg cggccaggta tccagcaagc
      661 caagaaatgc cacctgttcg ccacgggcac gtagtcgcgc cgcaatgccc tgcgccagtg
      721 taccgccaag ggaatacccc agcaggtaat aagggccgtg cggttgttgt tcaagtaacg
      781 ttgccagatg cgcttcgcag acttcatcca ggttcgtcgc tgtctgcatg gggccatgag
      841 ggcgcggcga ctgaatgccg ataatcgacc atagtggatc gagataacgc gagagcacgc
      901 taaactgcca ggcaaaaccg gatgccggat ggaaacaaaa cagcgtcggg ccattacctt
      961 cacgcaacgg cagaatggtt tcgaatccca tgcgccggga gctgtcctct tcaccatcaa
     1021 taatcgttgc cagtttggcg acggttgacg cgaccatcac ctgccccggc gtcacctgac
     1081 gggcaaactg ccgacttaac tgcgctgcca gtttcattgc cagtagcgaa tgaccgccaa
     1141 gcgcgaagaa atcagcatcg gcatcctgca cgtcacaacc cagcaacgac gcgaatgccg
     1201 cagcgataat cgtttcactg cccgctttcg gcgcacgccc cggcgtttgt gccttaagtt
     1261 caggcaacgg taaggctttg cgatccagct tgccgttggc gctaagaggt aactgtggaa
     1321 gttgcagcag aacgaccggc gccatatgcg gcggcaatgt ttcgcgaagc tgtgcctgta
     1381 atgcgctggt atccaacggc agacctgatt gcgacaccag atagcccacc aactgacgcg
     1441 catcaccacc ggtggctgcc gcctggttaa tcacacaggc gtgggtaacg gcttgttcga
     1501 catccggcag cgcctgcatc acgcgatcga tttcgcccag ttcgatacgc tgcccgcgaa
     1561 tttttagctg atcgtcactg cgcccgaggt actccaccgc gccgttatcc agccagcggg
     1621 caacgtctcc ggtacggtac atccgttcac cagggacaaa aggatcggca atgaagcggc
     1681 tggcggtcag atcgggtcgt ccaagatacc cctgcgccag ttgaataccg gtgagataga
     1741 gatctcccgc cacacccggc ggcaccggat gcatcatcgc atcgagaatg cgcaagcccg
     1801 tattccacac cggataacca atcggcacac tgctgccgcg cacctgtgcc agttcctcgc
     1861 caaaagccgg ataccaactc acatctaccg ccgcttccgt cgggccatat agattatgca
     1921 acggcgcgcc cgttaactgt tgccattcgc ggcataaatc agccggtaag gcctcaccac
     1981 tacagaaaac ctgtttcaac gtcgcgcaat tctggcgagc ggtttgcggc gtcagcgatg
     2041 caacaaatgc cgccagcatc gacggcacaa agtgcgtggt cgttacgcca tattcggcaa
     2101 agaattgctg catagcgagc gggtcgcggt gcgcttccgg ttcagccatc accagtttag
     2161 ccccggcaat aaacggccag aaaaactccc acaccgagac atcaaaactg cacggcgttt
     2221 tttgggcaac gacatcttca cctgtaagtg gataatgatt ttgcatccac aacaggcggt
     2281 taacgatagc cgtctgcccg accattaccc ctttcggcct gccggtggag ccagaggtaa
     2341 agatgatata agccgtgtga tgcggttgtg aaagttgcag cggcgcactg ccctgcggtg
     2401 taagcggggc gttatagcaa aggttggtta aatctggaac atcggcaaag cgcggcagtt
     2461 gatcgtcggt ggtgattaac agcgacggac gcgcatcttc cagcatcatt ttcaggcgat
     2521 cgtccggata accggtatcc agcggtaacc aggccgcacc tgcttcaaca atcgcatgta
     2581 gcgccagggt caaaaagacc gagcgcggta atgccaccgc cacgctgtcc cctggtttaa
     2641 cgccgtgctc acgcagcaga ttcgccagcg ccaccacctg ctcgcgcatt tcccgatagc
     2701 tgaactggta acgcgcatct gccagcgccg gagcatccgg tgtttttgcc gcttgttctg
     2761 ccaccagcgc gctaagcgtg gtttctggaa tctcaacctg agtggcgttg atctgcgcca
     2821 gctgcgcata ctcacctggc agcataatat caacatcgcc gcacaacaga gccggatccg
     2881 cagcgaattg ggcgatcagc attttcaggc gttcagcatg ctggattaac gttggctcat
     2941 cgtaatgctg tttattggcg aggatctcaa tactcaaatc accgtgttca tccgggaaca
     3001 gggccagttc aaggtcatta accggaccgg ttgccagggt atgggtttgc gcctgaacac
     3061 caggaatatc cagttggtaa tcaaatacct tgatattgag taccggacca aacagcggtt
     3121 catcacctgc cgctcgcccg ctgtcacgga caatttgttc ggcatcgtaa cgttgatgac
     3181 gacgcatttt tttcagttgt gctgccagtc gggttgccag ttccggcagc gtttcttgtg
     3241 ccgcaatgtg aatacccaac ggtaaaacgt tgagcacggg tccggtagcc gtcagcgccg
     3301 ccgagcccag tcgacgcata aagataaatc cggcggcgta gtccatgcga ttgcacaatc
     3361 gccccagcca aaaggctgcc agcgcaaggg ctaaatcggt acgctgcaca cctgaaagtt
     3421 gcgtagccag ctggcggaat tccccgtcgg taaattccag tttcaggcgt agaatatctg
     3481 ccgaagcgct gcgccccgct aaaggtgccg gagaaagtga cgcgggcggc ggcagttgac
     3541 gacgctgttc cgcccagaat gccgcatcac gctgccaggc ttcgctttcg cggtattgct
     3601 ggtactcttc cactacatca gcgaaaggcg taaacggcga agcaggcgtt tgttcgccac
     3661 gcagcaatgc gcagtaaatg ttggcgatct gccgagtaat ggccgggaaa ctgaagccat
     3721 cgaccagcaa atggtgataa cgctgatacc agtaccagcg gttatctgcc acctgtatca
     3781 gctggtgaaa gaccagtggt ttaccgctat cgacgcgcaa atcttgttgc aaatccgcct
     3841 gcattaatgc ctgcgcagta ccgtgcggat caatattggt tcgcaggtcg ataatttctg
     3901 gcagttcgaa tatcagcgca tcatcgaccc attgccagac ttcgccgtta tcttccgtaa
     3961 aacgcatccg cagcgtatct gcttgcgcta atccggcaac caccgcgcgg gccagtaatg
     4021 gcgcatcaac ctctccggtt aactcaacgt aatgcgccac gctccaggcg gagggtaatt
     4081 ctgacagttt ttctgccatc cagatgccgg gctgtgcggc gaccaaaggt aaatgctggc
     4141 tcattgtgcc tcctgcaact gggtgaaatt cgccggtgtc agagtacgcc agtggacttc
     4201 cagccactgc tggcaggact cttgtgactg cggctgacac acaatgtccc agcctgccgg
     4261 taaggcgcat tgttgcggcc acagactgaa ttgcccctgc gcattgcgca aaatgtaaaa
     4321 cactccctgt ggatcatcga agggattact gaatgccata ttcaactcct gtcatggaaa
     4381 agtggttgcc agaggtcgat taacccctgc atcaagccac cgcgccaaca aagcgcatca
     4441 tgtccgccgt caacctgacg ccagaaaatg gattctttta tagggtgtaa ttgcgcatac
     4501 agcgcctgat tggcccgcat gatcatcggc tcgcgaatac ccgcttccag cacaatgcgc
     4561 agaccttcgg cgctaacttc accagctttt agcttttcaa gtaacacgcc ctcttgctgc
     4621 ccgccccgat gcggccacca gtacgatcct gactggctta atacacagcc aaagcgttca
     4681 ggccagtgca gtccggcata cagcgcggac agcccaccaa aactctgccc ggcgaccacg
     4741 gtgcgatcgg cgcgatcgct aaaaggggca atagctttca ccaggggtaa taactcttgc
     4801 tgtactgcca gccagaaatc cgcattacac ggcagttcgt gggcgcggtg cgtggtgtcg
     4861 atagcgtcga tcaacacata cacggcggga ggaagttgct gacgatgggt cagcgaaatc
     4921 agcacaggcc agacgggcat actttgcgcc caaaattcgc catcgagcaa aactgccagc
     4981 gggcgttctt ctgctgttac atcgccggtg gtaaaaatcc atacacgacg tgaatttttc
     5041 aaccgttcac ttttccagat aatttctttg gctggtattt ctggcgcttg cggacaatcc
     5101 catcccggtt gcagaggcgc ttgcggcatt tcgagtgcag aaacagcgtg ccctcgcccg
     5161 cctttccagc tttgtggatt cagcggatcg gctattgcct ggggtaatag ttttcgccag
     5221 ccttcacgta attcgaggcg atcgggggat ggtgcagaaa aaatgtcatc gcgttcggtg
     5281 ggaataaagc agtagctgcc gcgccagttg gcattgagtt gtgtcgtcca ctgccagaca
     5341 tcagtacctg caattcgctg catcgactgg ggctggctgt tctgatgatg atcggtcaca
     5401 ccagtgatgt agatccatac gcgctttatc gtcgagtatt cttcagaacc ttggggatca
     5461 cgccaccaga aagtgacctc aaacatttcg tcattcagac gctgccattc cgggccatgt
     5521 ttcgactgcc accagctctc acttcctact tttaacgccg tcacaccata accccatgtt
     5581 tactgtgcaa tttttcattg attgcagaaa tatattgata atattattga taactatttg
     5641 catttgcaat agcgtaatgg cgcgccgtgg gaagcgcgga cattaattaa ccaactgcac
     5701 tgcgtgtctt tcaggatcaa aggttttcgc ggtagcggga tgcgtcgtgt tgatgacgac
     5761 catgcccgac agttgcaatt cgtggcaaaa atgcaggaat aaaacaatga acaagaagat
     5821 tcattccctg gccttgttgg tcaatctggg gatttatggg gtagcgcagg cacaagagcc
     5881 gaccgatact cctgtttcac atgacgatac tattgtcgtt accgccgccg agcagaactt
     5941 acaggcgcct ggcgtttcga ccatcaccgc agatgaaatc cgcaaaaacc ccgttgcccg
     6001 cgatgtttcg gagatcatcc gtaccatgcc aggcgttaac ctgaccggta actccaccag
     6061 tggtcagcga gggaataacc gacagattga tattcgcggt atgggtccgg aaaacacgct
     6121 gattttgatt gacggcaagc cggtaagcag ccgtaactcg gtgcgtcagg gctggcgtgg
     6181 cgagcgcgat acccgtggtg atacctcctg ggtgccacct gaaatgattg aacgtattga
     6241 agttctgcgt ggtccggcag ctgcgcgtta tggcaacggc gcggcgggcg gcgtggttaa
     6301 catcattacc aaaaaaggca gcggcgagtg gcacggctcc tgggacgcat atttcaatgc
     6361 gccagaacat aaagaggaag gtgccaccaa acgcactaac tttagcctga ccggtccgct
     6421 gggcgacgaa ttcagcttcc gcttgtatgg caacctcgac aaaacccagg ctgacgcgtg
     6481 ggatatcaac cagggtcatc agtccgcgcg tgccggaacg tatgccacga cgttaccagc
     6541 cgggcgcgaa ggggtgatca ataaagatat taatggcgtg gtgcgctggg acttcgcacc
     6601 attgcaatcg ctggaactgg aagcgggtta cagccgccag ggtaacctgt atgcgggcga
     6661 tacccagaac accaactctg acgcttacac tcgctcgaaa tatggcgatg aaaccaaccg
     6721 cctgtatcgc cagaattact cgctgacctg gaacggtggc tgggataacg gcgtgaccac
     6781 cagcaactgg gtgcagtacg aacacacccg taactcgcgt attccggaag gtctggcggg
     6841 cggtaccgaa gggaaattta acgaaaaagc gacacaggat ttcgtcgata acgatcttga
     6901 tgacgtgatg ctgcatagcg aagttaacct gccgattgat ttcctcgtta accagacgct
     6961 gacgctgggt acggagtgga atcagcagcg gatgaaggac ttaagttcca acacccaggc
     7021 actgaccgga acgaataccg gtggcgctat tgatggcgtg agtgccaccg accgtagccc
     7081 gtattcaaaa gcagaaattt tctcgctgtt tgccgaaaac aacatggagc tgactgacag
     7141 caccatcgta acgccggggc tgcgtttcga tcatcacagt attgtcggca ataactggag
     7201 cccggcgctg aacatatcgc aaggtttagg cgatgacttc acgctgaaaa tgggcattgc
     7261 ccgcgcctat aaagcgccga gcctgtacca gactaacccg aactacattc tctacagtaa
     7321 aggtcagggc tgctatgcca gcgcgggcgg ctgctatctg caaggtaacg atgacctgaa
     7381 agcagaaacc agcatcaaca aagagattgg tctggagttc aaacgcgacg gctggctggc
     7441 gggcatcacc tggttccgta acgattatcg caataagatt gaagcaggct atgttgctgt
     7501 agggcaaaac gcagtcggca ccgatctcta tcagtgggat aacgtgccga aagcggtggt
     7561 tgaaggtctg gaaggatcgt taaacgtacc ggttagcgaa acggtgatgt ggactaataa
     7621 catcacttat atgctgaaga gtgaaaacaa aaccacgggc gaccgtttgt cgatcatccc
     7681 ggagtatacg ttgaactcaa cgctgagctg gcaggcacga gaagatttgt cgatgcaaac
     7741 gaccttcacc tggtacggta agcagcagcc gaagaagtac aactataaag gtcagccagc
     7801 ggttggaccg gaaaccaaag aaattagtcc ttacagcatt gttgggctga gcgcgacgtg
     7861 ggatgtgacg aagaatgtca gtctgaccgg cggcgtggac aatctgttcg acaaacgttt
     7921 gtggcgtgcg ggtaatgccc agaccacggg cgatttggca ggggccaact atatcgccgg
     7981 tgccggggcg tatacctata acgagccggg acgtacgtgg tatatgagcg taaacactca
     8041 cttctgatgc taacgtcaga ttgttgacaa agtgcgcgtc gttcatgccg gatgcggcgt
     8101 gaacgcctta tccggcctac aaaaatcttg ccaattcaat atattgcagg accatgtagg
     8161 cctgataagc gtagcgcatc aggcagtttt gcgtttgtca tcagtctcga atatggtcga
     8221 tatgaaaact acgcatacct ccctcccctt tgccggacat acgctgcatt ttgttgagtt
     8281 cgatccggcg aatttttgtg agcaggattt actctggctg ccgcactacg cacaactgca
     8341 acacgctgga cgtaaacgta aaacagagca tttagccgga cggatcgctg ctgtttatgc
     8401 cttgcgggaa tatggctata aatgtgtgcc cgcaatcggc gagctacgcc aaccggtgtg
     8461 gcctgcggag gtatacggca gtattagcca ctgcggggct acggcattgg ccgtggtttc
     8521 ccgtcaaccg attggcgttg atatcgaaga aattttttct gcgcaaacag cgaccgaatt
     8581 gacagacaac attattacac cagcagaaca cgagcgactc gcagactgcg gtttagcctt
     8641 ttctctggcg ctgacactgg cattttccgc caaagagagc gcatttaagg caagtgagat
     8701 ccaaactgat gcaggttttc tggactatca gataattagc tggaataaac agcaggtcat
     8761 cattcatcgt gagaatgaga tgtttgctgt gcactggcag ataaaagaaa aaatagtcat
     8821 aactctgtgc caacacgatt aattgacaac atctggtacg attcgcccgc agccatcact
     8881 gactgcgggc gaaagtgtaa agcaggtgcc ttaccactct gacctgacaa ccggatatgc
     8941 ggggattgct ccccgcacaa cggctacttc ttcggttcgt aagcgagaac agccttaaac
     9001 tcaatattac gttcctttac ggtgaactca cacagcgagt ctccgaccag aagcgtaaat
     9061 cccagcaccg tcaaacacag cactatgact gccacaaggg catatttcgt cagcatgttt
     9121 atattgcctc catgcgaaga agggactacc atccgagttg ttggggctcg gagtggcagc
     9181 cccaggattg atagaaatat ctcctggggc ttcatctttc cagacctcag agttaacctg
     9241 aaaccggaaa gcttcaggca cccgccgtta tcttaccctt ccttagcaaa atagctgttt
     9301 atttatacag tgaatcctgt ttcggacttc tgtcggcaaa actcatcatt gctcttaaaa
     9361 cgaaatcgcc cgcagccatc actgaccgcg ggcgaaagtg taaagcaggt gccttaccat
     9421 cctgacctga caaccggata tgcggggatt actccccgca caacggctac ttcttcggtt
     9481 cgtaagcgag aacagccctg aactcaatat tacgttcctt caccgtaaac tcacacagcg
     9541 agtctccgac cagaagcgta aatcccggta ccgtcaaaca cagcactatg actgccacaa
     9601 gggcatattt cgtcagcatg tttatattgc ctccatgcga agaaggaact accatccaaa
     9661 ttgtctggtt tggagtggca gccccagggt tgatagaaat atctcctgga gcttcatctt
     9721 tccagacctc agagtaaacc tgaaaccgga aagcttcagg cacccgccgt tatcttaccc
     9781 ttcctcaaca aaatagctgt ttatttatac agtaaatcct gtttcggact tctcactcct
     9841 catttaatca actgaagctg agcccgtttt ttctgcatgc ctcaatcgtc aaaattcttc
     9901 gccaccagcc agccgtgcca taacttgtca cattgcaaaa gtagccaaat tgcttcagtc
     9961 accatcacca aaaaaagaaa cattccagaa cttccgcatc tcgcccagcc gctccgactt
    10021 ctatactgaa tagaaaactc caacataaga gaaacctatg ccattacccg attttcatgt
    10081 ttctgaacct tttaccctcg gtattgaact ggaaatgcag gtggttaatc cgccgggcta
    10141 tgacttaagc caggactctt caatgctgat tgacgcggtt aaaaataaga tcacggccgg
    10201 agaggtaaag cacgatatca ccgaaagtat gctggagctg gcgacggatg tttgccgtga
    10261 tatcaaccag gctgccgggc aattttcagc gatgcagaaa gtcgtattgc aggcagccgc
    10321 agaccatcat ctggaaattt gcggcggtgg cacgcacccg tttcagaaat ggcagcgtca
    10381 ggaggtatgc gacaacgaac gctatcaacg aacgctggaa aactttggct atctcatcca
    10441 gcaggcgacc gtttttggtc agcatgtcca tgttggctgt gccagtggcg atgacgccat
    10501 ttatttgctg cacggcttgt cacggtttgt gccgcacttt atcgcccttt ccgccgcgtc
    10561 gccatatatg cagggaacgg atacgcgttt tgcctcctca cgaccgaata ttttttccgc
    10621 ctttcctgat aatggcccga tgccgtgggt cagtaactgg caacaatttg aagccctgtt
    10681 tcgctgtctg agttacacca cgatgatcga cagcattaaa gatctgcact gggatattcg
    10741 ccccagtcct cattttggca cggtggaggt tcgggtgatg gataccccgt taacccttag
    10801 ccacgcggta aatatggcgg gattaattca ggccaccgcc cactggttac tgacagaacg
    10861 cccgttcaaa cataaggaga aagattacct gctgtataaa ttcaaccgtt tccaggcctg
    10921 ccgctatggg ctggaaggcg tcattaccga tccgtacact ggcgatcgtc gaccactaac
    10981 ggaagacacc ttgcgattgc tggaaaaaat cgccccttct gcacataaaa ttggtgcatc
    11041 gagcgcgatt gaggccctgc atcgccaggt cgtcagcggt ctgaatgaag cgcagctgat
    11101 gcgcgatttc gtcgccgatg gcggctcgct gattgggctg gtgaaaaagc attgtgagat
    11161 ctgggccggt gactaagccg ggaattgcct tacagaagaa tatccctgac aatggtgttt
    11221 taacttacat ttaacaatgt tatgaaacac cctttagaaa ccttgaccac cgcagcaggc
    11281 attttgctga tggctttcct ctcttgcctg ctgctgcccg cccccgcact ggggcttacg
    11341 ctggcacaaa aactggtgac cacgttccat ctgatggatc ttagtcagct ttacacttta
    11401 ttgttttgtc tgtggttttt agtgctgggc gctattgagt attttgttct gcgctttatc
    11461 tggcgacgct ggttctcgct ggcggattaa acgtcgatta tcgccgtact tcgcggcata
    11521 ctttgcttat tctccttcgc ctaaaggaac gtttatggat aagcaatcac tgcacgaaac
    11581 ggcgaaacgc ctggcccttg agttaccctt tgtcgagctt tgctggcctt ttggcccgga
    11641 gttcgatgtt tttaaaattg gcggcaagat ttttatgctg tcgtcggagc tacgcggcgt
    11701 cccctttatc aatctgaagt ccgatccaca aaaatccctg ttaaatcagc aaatataccc
    11761 aagcattaag ccagggtatc acatgaataa aaagcactgg atttcggtgt atcccggcga
    11821 ggaaatctcc gaagcgttac ttcgcgatct gatcaacgat tcgtggaatc tggtggttga
    11881 tggtctggct aaacgcgatc aaaaaagagt gcgtccaggc taaagcggaa atctatagcg
    11941 catttttctc gcttaccatt tctcgttgaa ccttgtaatc tgctggcacg caaaattact
    12001 ttcacatgga gtctttatgg atatcatttc tgtcgcctta aagcgtcatt ccactaaggc
    12061 atttgatgcc agcaaaaaac ttaccccgga acaggccgag cagatcaaaa ctctcctgca
    12121 atacagccca tccagcacca actcccagcc gtggcatttt attgttgcca gcacggaaga
    12181 aggtaaagcg cgtgttgcca aatccgctgc cggtaattat gtgttcaacg aacgtaaaat
    12241 acttgatgcc tcgcacgtcg tggtgttctg tgcaaaaacc gcgatggacg atgcctggct
    12301 gaagctggtt gttgaccagg aagatgctga tggccgcttt gccacgccgg aagcgaaagc
    12361 cgcgaacgat aaaggtcgca agttcttcgc cgatatgcac cgtaaagatc tgcatgatga
    12421 tgcagagtgg atggcaaaac aggtttatct caacgtcggt aatttcctgc tcggcgtggc
    12481 ggctctgggt ctggacgcgg tacccatcga aggttttgac gccgccatcc tcgatgcaga
    12541 atttggtctg aaagagaaag gctacaccag tctggtggta gttccggtgg gtcatcacag
    12601 cgttgaagat tttaacgcta cgctgccgaa atctcgtctg ccgcaaaaca ttaccttaac
    12661 cgaagtgtaa ttctcccttg ccgggcatct gcccggctat ttcccctcag attctcctga
    12721 tttgcataac cctgtttcag ccgtcatcat aggctgctgt tgtataaagg agacgttatg
    12781 caggatttaa tatcccatgt tgaagattta gcgggtattg agatcgatca caccacctcg
    12841 atggtgatga ttttcggtat tatttttctg accgccgtcg tggtgcatat tattttgcat
    12901 tgggtggtac tgcggacctt cgaaaaacgt gccatcgcca gttcacggct ttggttgcaa
    12961 atcattaccc agaataaact cttccaccgt ttagctttta ccctgcaggg gattatcgtc
    13021 aatattcagg cggtattctg gctgcaaaaa ggcaccgaag cggcagatat tctgactacc
    13081 tgcgcgcagt tgtggatcat gatgtatgcg ctgctttcag tcttctcgct gctggatgtt
    13141 attttgaatc tggcgcagaa attcccggca gcatctcagt taccgctgaa agggatattt
    13201 caggggatta aactgatcgg cgcgattctg gtcggcattt tgatgatctc gctgctgatt
    13261 ggtcagtcgc cagcgattct gatcagcggt cttggtgcaa tggctgccgt gctgatgttg
    13321 gtatttaaag atccgattct tggtctggtg gcaggtattc agctttccgc gaacgatatg
    13381 ctgaaactgg gcgactggct ggagatgccg aaatacggcg cggatggcgc ggtgatcgat
    13441 attgggttaa ccaccgtcaa ggtgcgtaac tgggacaata ccattaccac tattcccacc
    13501 tggtctctgg tttctgactc ctttaaaaac tggagcggga tgtcagcatc tggcgggcga
    13561 cgtattaagc gcagtatcag tattgatgtc accagtattc gttttcttga tgaagacgaa
    13621 atgcaacgtc tgaataaagc gcatttgtta aagccttatt taaccagccg ccatcaggaa
    13681 attaatgagt ggaatcgcca gcaaggttct acggagtcgg tattaaacct gcgccgaatg
    13741 accaatattg gaacctttcg tgcctatctg aacgaatatc tgcgtaacca tccgcggatt
    13801 cgtaaagata tgaccttaat ggtacgccaa ctggctccag gtgataacgg tttaccgctc
    13861 gagatctatg cgtttaccaa caccgtggtg tggctggaat atgaaagcat tcaggctgat
    13921 atattcgatc acatatttgc gattgtcgaa gagtttggtc tgcgacttca tcagtcgcca
    13981 accggcaatg atattcgctc tctggcgggt gcatttaagc agtaattaaa aaaaccgctc
    14041 tcatcgaatg gatgagagcg gacatcgttt tgtcggatgt agcgtgaatg ccttatttcc
    14101 gacgcagcgt tttaaatgct acaaacagga atacaatcca caccggcagc aggatcgctg
    14161 acaagcgcat atcatccatc gtgcacatca gcagcaaaat catggcgagg aaggcgatgc
    14221 aaagatagtt gccgaacgga tagagcagcg ccttaaactg tgtttcacgc ccctgacgtc
    14281 gcatcgctgc acgaaaacgc agatgcgcca gacagatcat aatccagttc aacagcagcg
    14341 ttgctaccac cagcgccatc agcagaccaa acgctttttg cggcagcaga tagttgatta
    14401 acaccaccag cgaagtgatc gctccggaaa gcatcagcga gttaatcggc acgccgcgac
    14461 ggctaacacg ggtaaggaac ttcggcgcat taccctgcac agaaaggcca aacagcatgc
    14521 ggctgttaga gtaaaccccg ctgttataca ctgacagcga tgctaccaga atgacgaagt
    14581 tcagcgcaga agctaccacg ttgctgtcga gattatggaa aatcatcaca aacgggctac
    14641 tgttggattt cacttccacc cacggataga gcgccagtaa aaccaccagt gaaccgatgt
    14701 aaaacagcag gatgcgatac accacctgat ttaccgcttt tggaatgctt ttttccggat
    14761 cgcgcgcttc agcggcagta atcccaatca gctccagacc gccgaaggag aacataatta
    14821 ccgccagcga caaaatcagc ccattccagc cggtggcgaa gaaaccaccg tagcgccaga
    14881 ggttgtcgat actggctttc tcgccgccgt gaccagaaaa cagcagccac aggccaaagc
    14941 cgatcatacc gatgattgcc agcactttaa tcagcgcaaa ccagaactcg gtttcgccat
    15001 ataagcgcac gttcaccagg ttaacggcgt tgatgataat aaagaaggcg gcagcccaaa
    15061 tccacgttgg aacatccggg aaccagtact gcatatagat gcccgcagcg gtcagctctg
    15121 ccattcccac cagcacgaac atcacccagt agttccagcc ggagaggaac ccggcgaacg
    15181 gtccccagta tttataggca aagtgggcaa atgaaccgga taccggctct tcgaccacca
    15241 tctcgccgag ctggcgcata atcaggaaag cgatgatccc ggcgacgccg tagcccagca
    15301 atacagccgg acccgccatc tgaatcgccg ggccaatgcc aagaaacaga ccagtaccaa
    15361 ttgcgccacc cagcgcaatc agttgaatat gacggttatg taatccgcga tgaagcgtcg
    15421 gctcttgatt cgacgcagta tcttccgata cgtttgacgc gtttttcacg cctttcccct
    15481 gtgtgtcttt tttgttgagg ggcacctttt aacatttagt gcccatcgtc gcaagacaca
    15541 atccacacgg ttaaaccggg tatcctgctt ttatttccgt acccgatgtc ggtgcagcca
    15601 catcagctta tacgctgccg ggataataaa cagcgacagc aaaggcgcgg tgatcatgcc
    15661 gccaatcatc ggcgcggcaa tccggctcat cacctctgat cctgcccccg ttccccacag
    15721 aatcggcagc agaccagcga taatcaccgc caccgtcatc gctttcgggc gcacgcgcag
    15781 gaccgcgccg tgatgtaacg cctcatccag cttctgctcg ctgaatgttt gcggattatt
    15841 caacgacggc tcggcctcta tggcgtgacg taaatacatc agcatcacca cgccaaattc
    15901 ggcggcgacc ccggcaaggg caataaaacc ggtgcccgtc gccacagaaa gatgaaagcc
    15961 catccaccac agcaaccaga tgccgcccac cagcgcaaac ggtacgctgc tgataatcag
    16021 caacgcttcg cccacccgac ggaacgccag atacaacagc acgaagatga tcatcaacgt
    16081 catcggcacc atgagtttca gcttatggtt ggcgcgctcc agtagctcga actgcccgga
    16141 gaatgccacg ctggtgcccg gttttaactg cactttttca gctatcgctt tttgcaaatc
    16201 gtgaaccacc gacaccatgt cacgatcgcg ggcatcgata taaatccagc tcgtcgggcg
    16261 cgcattctcg gttttcagca tcgacggtcc ggtagagact ttaacgtcgg ccacgtctgc
    16321 cagggtgatt tgctgcttca tcggcgtcag gatcggcaac tggcgcagtg cctgcggact
    16381 atcgcgccag ctttgcggat aacgcagatt aattggataa cgggcaatcc cttccaccgt
    16441 ttcgccaacc atcgccccgc ccaccgcaga agtcacaaac aactgcacat ccgccaccgt
    16501 cataccgtaa cgtgcggctt tttcacggtt aatctcaacg ttgatatagc gcccaccttc
    16561 cagccgctcg gcaagcgcag aagccacgcc tggcaccgtt cgtgccacct cttcaatttg
    16621 ctccgccatc gcgtcgatat ccgccagcac agtgccggaa acttttatgc cgatagggct
    16681 tttaatgccg gttgagagca tatcgatacg gttacgaatt ggcggcaccc acagattcgc
    16741 cagccccggc agacgcacgg tgttatccag ttcctcaatg attttgtcca ttgtcatgcc
    16801 tggccgccac tgctcctgcg gcttaagctg gatggtcgtt tctaccatct ccagcggagc
    16861 agaatcggtg gcggtttccg ctttcccggt tttgccaaat acccgcgcca cttcaggtac
    16921 gctcataatt agcttgtcgg ttttttgcag catactcgcc gcctctgctg cggaaatccc
    16981 cggcagcgtc gatggcatat acaacaagtc gccttcattg atctgcggta aaaattcccc
    17041 gccaacttta ttgagcggcc agagaaccgt cagcaccgaa agcgctgcca ccaacagcgt
    17101 ggttttcggc cagtgcagta ctttcagcaa cagcggatga taaacacgaa tcaaaaagcg
    17161 attgagcggg ttactgcttt ccggcggaat tttgccacgg atccagtaac ccatcaggat
    17221 agggatcact acgatcgcca gcagcgccgc acccgccatc gcatacgttt tggtgaacgc
    17281 cagcgggcca aacagacgcc cttcctgccc ttccagggtg aagatcggga taaacgacag
    17341 cgtgataatc agcagactga taaacagcgc cggccccact tcaacagatg cattggtaat
    17401 cacctgccag cgcgttttat tatccagcgt ggcgtcagga tgctggtgct gccactcttc
    17461 cagccgttta tgtgcattct cgatcatgac gatagcggca tcgaccatcg ccccgacggc
    17521 aatcgcaatg ccacccaacg acataatatt ggcattcagc ccctggaagt gcatgacaat
    17581 aaaagcaata cacaacccca gcggcaacga aataatcgcc accagcgccg agcgcacatg
    17641 ccagagaaac agcgcacaga ctaccgccac cacaataaac tcttccagca acttgccgct
    17701 gaggttgtcg atggcccggt caatgagctg gctgcgatcg tatgttgtaa ctatctccac
    17761 gccttccggc agactgcttt tcagcgtttc cagtttgtcc ttcacggcgg cgatcacttc
    17821 acgggcgttt ttgccggagc gtaggatcac caccccgccc acaacttcgc cgttcagttc
    17881 ggcaatgccc cggcgcatct ccgggccaac ctggatcttc gcaacatcac gcagataaac
    17941 aggcacgcca ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag
    18001 atagccgctg gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc
    18061 ttcctggttt gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc
    18121 caggcgctga ggatcgataa ccacctgata ctctttcacc acgccgccca ccgacgccac
    18181 ttccgcaacg tcagggatgg tttttagctc atatttgaga aaccagtcct gcaatgagcg
    18241 taaatcggcc agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc
    18301 aacacccgtg gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac
    18361 ctggttgagg tactccagca cccgcgaacg cgcccagtac ggatcggtac catcttcgaa
    18421 aatgacatac acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg
    18481 caccgacaac atggtggtag ttagcggata agtcacctga ttttcaacga tttgcggtgc
    18541 ctgaccggga tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc
    18601 cactggcgtg ttaatgatgg tccaggtgcc ccagatactc agaaataacg cgcccatcag
    18661 caccaaaaaa cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct
    18721 caatgcgcat gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct
    18781 tcggaatcaa tcaggaacag gccgctggaa accacctttt caccttccgc cagaccagag
    18841 cgtaatgcgg tgacaccttg cgatgcctgg aaaacggcaa cgcgttttgg tacaaagcgc
    18901 ccgtcggcat caacggtaat cacccgctgt tcgctgccgg tatcaatcag cgcctgtgac
    18961 ggaatgagca gcatcggttc gctggcggta ttgagttgca accaggcgtt cattcccggt
    19021 tttagcgcct cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca
    19081 tccacgccag gtagtagcgt ccatttgcgg atggtgagtg ttttatcagg tcgcgccgga
    19141 acggtgagcg taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca
    19201 gcagtgaccc acaccgggtc cataccctga attttcgcta ccacgttatc tttggcgata
    19261 ttcattcccg cgcgcagatc aaacgcggtg atcacgccat caatgggtgc tttgagcgta
    19321 aagcgagtct ggattttttg cgtggcgatc aggcggcgaa tatccgcctc cggcattccc
    19381 gccagtcgca gccgctcaag aatgccttcg gtctgggtcg ccgtaccgcc ggtttcgcgc
    19441 agcagtaaat actcactctg cgcttccacc cagtcgggaa tggtcaggtc gagaagcggc
    19501 gcgccctttt gtactttatc gcccacggta agcggataca ccttgtcgat aaacccggcg
    19561 gcgcgagcct gcacaatggc atactgatac tcgttgtaac tgacattcgc cgggaaactc
    19621 tgggcaaaag tcagcggccc gcgcgtgacg gtagccgttt tcacccccag attctgcgtc
    19681 tgagtcgggt caatgcgcac accagacgca gaactctctt catcggcata tttcggcacc
    19741 agatccatat ccataaacgg cgatttacct ggtttatcga accgcgtatt gggatacatc
    19801 gggtcgtacc agaataagat tttacgttct gcggtcgacg ttttttctgc aggcggttcc
    19861 gcctttgcaa accaggtaaa acctgccgca gaaataatac cgcccgcgat catgctgccg
    19921 ataataagcg cgattttttt catctcatta aacctgggtt actggctgac tttaatatcc
    19981 tgtaataaag aaaggttgcc ctgctggaca aaattaaacg ccactttgtc gccggtttta
    20041 attccactca ttttcgtctg cggggtgatg gtaaagcgca tggtcatctc cggccagttc
    20101 acggcagcaa tcggatcgtg atggatggtg atttttttgc tttccagatc aaaacctttt
    20161 accacgccgg tggcactaat gacctgtggt tgtgcttcgc tcatggtttc atgatgatgt
    20221 tcgttagcct gggcattaaa gccaataacg gtaaacagac tgaacattgc gacttgcagt
    20281 gcttttttca tttattttct cctggagtta aaaattattg ctgccaaccg ccaccaagtg
    20341 cggtatataa agaaatttcg ttgacctgac gggcataatt caggtcaagt acggtttgtc
    20401 gggttgcaaa taaagaacgc tcggcatcca gcacttccag ataacttact gcgccgtgct
    20461 gatataatgc ccgcgcccgt tgcaaagtaa tttgcagcga tgccagataa cgctgctggg
    20521 cgctaatttg atcgttcagg ctttgacgta atgccagcgc atctgccact tctttaaagg
    20581 cgttctggat tttctgttca taattcacta ccgactgctg ctggcgaatt tcggcgatat
    20641 ccagattggc ctggttgcgt ccggcattaa aaatggggat ctcaatttta ggaataaaat
    20701 tccacatccc gctgctggcg ttaaataatg acgacagatc gctgctggcg gttgatattc
    20761 cgctggtcag gctgatagac gggaaaaatg ccgcgcgtgc agcaccaata ttggcattag
    20821 ccgccattaa cgcatgttca gcttccataa tatccgggcg ctgcaataag atttgcgacg
    20881 acaagcccgc cggtaattta acgctttgca ggctgtcgct gtttactgtc tgcgcttgcg
    20941 gcagcttgcc gtagcttccc agtaacagtt gcaatgcatt attcgcctgc gccagttccc
    21001 cctgacgttt agcgatgtcg ctgcgggtac tttcaatcac cccgcgagcc tgttccagcg
    21061 ccagaacatt actgctacct gtcaacagtt gtttttcgac aaacgcatat gactgctgat
    21121 aattacgcag cgtttcttcg gctatttgca attgcgcata cgccagctgc tgattgaaat
    21181 agctttgcgc gacattagaa accagcagaa tatgcaccgc gcgctgagct tcctcagtgg
    21241 ctaaataatt ttgtcgctcg gcttcgctca tgttctttaa gcgcccgaaa aaatcgaggt
    21301 caaagctggc gttaaggcca gtagaaaact cccgcgtcgt ggctgaatcg cctttaagat
    21361 tgccgctcca gctgccgctg ccctcaccat tgagctgtgg gtagcggtcg gcatcagtca
    21421 gacgatattg cgcccgtgcc tcctgcactt tcagcgtcgc catgcgcaaa tcccggttat
    21481 tctccagcgc ctcgctaatc agcgtcttca cctgattatc aacaaaaaag gtgcgccagc
    21541 ccgcgttctg atagttatct gctgcgttaa ccaggccgtt ctggctgagt gagaactgct
    21601 gcggtacggg cattgccggg cgctgataat ccggtgccag tgaacaaccg gttagcgcaa
    21661 gggccacaca aaatggcaga agtttacaag gagacatagg ctcataattt ctggtgattt
    21721 tataccgcca actttactcg ccaggctctg attttccggt gacaggaaaa tgacaaaatt
    21781 gtcattttgc caataagcga ttgccatctg atcccgctac tctagaattg cccgggcaac
    21841 atgcggagga aatatgaaac tgttgattgt cgaagatgaa aagaaaaccg gagaatactt
    21901 gaccaaaggg ttaaccgaag ccggttttgt ggtcgatttg gccgacaacg ggctgaatgg
    21961 ctaccatctg gcgatgaccg gagattatga cctgataatc ctcgatatta tgctgccgga
    22021 cgtgaacggc tgggatatcg tgcgcatgtt gcgctccgcc aataaaggga tgccgattct
    22081 gttgcttacc gcgcttggca ccattgaaca tcgcgtcaag gggctggagt tgggggccga
    22141 tgactatctg gtgaagccgt tcgcgtttgc tgaactgctg gcgcgggtgc gcactctcct
    22201 gcggcgcggg gcggcggtga ttatcgaaag tcagtttcag gttgccgatt tgatggtcga
    22261 tctcgtcagc cgcaaagtca cccgcagcgg cacgcgcatc actctgacca gtaaagagtt
    22321 tactctgctg gagttttttc tccgccatca gggcgaagtg ctgccccgct cgcttatcgc
    22381 ctcgcaggta tgggacatga attttgacag cgacactaac gccattgatg tggcggtgaa
    22441 gcggctacgc ggcaaaatcg acaacgactt tgagccgaag ctgattcaaa ccgtgcgcgg
    22501 cgtgggttac atgcttgagg tgccggatgg tcagtaagcc atttcagcgc ccgttttcgc
    22561 tggcaacccg cctgaccttt tttatcagcc tggccaccat cgcggcgttt ttcgcctttg
    22621 catggatcat gatccactca gtaaaagtgc attttgccga gcaggatatt aatgatttaa
    22681 aagagattag tgccaccctt gaacgcgtac taaatcaccc tgacgaaacg caagcccgac
    22741 gcttaatgac gctggaagat atcgtcagtg gttattccaa tgtgttgatt tccctggcag
    22801 atagccacgg taaaacggtg tatcactccc ccggtgcgcc tgatatccgc gagtttgcgc
    22861 gcgatgccat acccgataaa gacgcgcggg gcggcgaggt gtttctcctc tccggcccga
    22921 cgatgatgat gccaggccac ggtcacgggc atatggaaca cagcaactgg cggatgatca
    22981 gtttgccagt tggcccactg gttgacggca aaccgattta tacgctctac atcgcgcttt
    23041 cgatcgattt tcatcttcat tacataaatg atttgatgaa taaacttatt atgaccgcat
    23101 cggtcatcag catcctgatc gtctttatcg tgctattggc ggttcataaa ggtcacgcgc
    23161 cgatccgcag cgtcagccgt caaatccaga atatcacctc gaaagatctc gacgtccgcc
    23221 ttgacccgca gaccgtgccg attgagctgg aacagctggt actgtcgttc aaccatatga
    23281 tcgagcgtat tgaggatgtc tttacccgcc agtccaattt ctcagcggat atcgcccacg
    23341 aaattcgcac accaattact aatctcataa cgcaaacgga aatcgccctc agccagtcgc
    23401 gcagccagaa ggagctggaa gatgtgctct actctaatct cgaagagctg acgcgaatgg
    23461 cgaaaatggt cagcgatatg ctgtttctcg cccaggccga taacaaccag ttaatccccg
    23521 aaaagaaaat gctcaacctg gcggatgaag tcggcaaagt gttcgatttt ttcgaggctt
    23581 tagcggaaga tcgcggcgtg gagctgcagt ttgttggcga cgagtgccag gttgcgggcg
    23641 atccgctgat gctgcgtcgg gcgttaagca acctgctttc gaacgccctg cgttatacgc
    23701 cacccggaga ggctattgta gtgcgctgcc agacggtcga tcatctggtg caagttatcg
    23761 tcgaaaaccc cggtacgccc attgcgcccg agcacttacc gcgattgttt gaccgtttct
    23821 accgcgttga cccctcccgc caacgaaaag gcgaaggtag cggcattggg ctggcgatag
    23881 tgaaatcaat tgttgtcgcg cataaaggca cggttgcggt tacgtcaaat gcgcggggga
    23941 caaggtttgt gattgtgttg ccggagaggg ggtgattatt aagttcaaat tcagggcgag
    24001 ggtttacggc gtttttcgct aaatttccat cattataaac aagccaccta tcatataaag
    24061 caatgaataa ttaaatcgcg attaatcatc attaattttt atattattca cccaatccat
    24121 ttataaagtg aatttgataa attcctaatt taaaatatcc actattgagc cagtatttaa
    24181 caacgttaag atgtgcaatg tgcagatcat gttatcaggg tggagcggta gcatatattt
    24241 tttaagcaat cattcatttt aatgatgtta attgggttat ttagttgtga ataaaaacat
    24301 attatgaata ttggtttaac tttgcgtttt tgttactcgc tgtgatgtgc gcacctgtcc
    24361 tgaataacgt caggaaacaa taagttgagc ttgtgattat ttttttgact gaacagacac
    24421 ccgtttctgt aaagtaatat ttaaacaaaa tcctcacata atcatagcat tttttgttcc
    24481 ttccctaata acgtcttaag tcgtcccgct tatctcatta tccgcagttg tacggtgcgg
    24541 attattattc attttaccgg aggttgctat gtcaaccgga ttacgtttca cactggaagt
    24601 ggacggcctg ccaccggacg cttttgcggt ggtttccttt catctcaccc agtcactctc
    24661 ttcgctcttt tccctcgatc tctccttggt cagccagcag tttctctccc ttgaatttgc
    24721 gcaggtgctg gacaaaatgg cctacctgac aatatggcag ggtgatgatg tacagcgccg
    24781 ggtgaaaggt gtggtgacct ggtttgaact gggggagaac gacaaaaacc agatgctgta
    24841 cagcatgaag gtgcacccgc cgctgtggcg tgccggtctg cgccagaact tccgtatctt
    24901 ccagaacgag gacatcaaaa gcatcctcgg cacgatattg caggaaaacg gggtgaccga
    24961 gtggagtccg ctgttcagtg aaccgcatcc ttcccgtgag ttttgtgtcc agtacggtga
    25021 gactgattac gatttcctgt gccggatggc ggcggaggaa ggcatcttct tttatgagga
    25081 gcatgcttac aaaagtaccg accagagcct ggtgctgtgc gacacggtcc gccatctgcc
    25141 cgaatctttt gaaatcccct ggaacccgaa cacccgtacc gaggtgagca ccctctgcat
    25201 cagccagttt cgctacagcg cacaaatccg cccttcttcc gtggtgacca aagactacac
    25261 ctttaaacgc cccggctggg caggacgttt tgatcaggaa ggccagcacc aggattacca
    25321 gcgcacgcag tatgaggtgt atgactaccc cggacgtttc aagggcgccc acgggcagaa
    25381 ctttgcccgc tggcagatgg agggctggcg aaacaatgca gaaaccgcgc ggggaatgag
    25441 tcgctcgccg gagatatggc cgggacggcg aattgtgctg acggggcatc cgcaggcgaa
    25501 cctgaaccgg gaatggcagg tggtggcaag tgaactgcac ggcgagcagc cgcaggcggt
    25561 gccgggaagg cggggagcag ggacggcgct ggagaaccat tttgcggtga tcccggcaga
    25621 ccggacatgg cgaccccagc cgcggctaaa accgctggtg gacggtccgc agagcgccgt
    25681 cgtgacgggg ccggagggtg aggaaatctt ctgtgatgaa catggccgag tgcgggtgaa
    25741 attcaactgg gaccgttata acccggcaga ccaggacagc tcgtgctgga tccgtgtggc
    25801 acaggcgtgg gcaggcaccg gttttggcaa cctggcgata ccgcgtgtgg gtcaggaggt
    25861 gatagtggac ttcctcaacg gcgatccgga ccagccgatc attatggggc gcacctacca
    25921 ccaggaaaac cgcacccccg gcagcctgcc ggggacgaag acgcagatga ccatccgttc
    25981 caaaacgtat atgggcagcg ggtttaatga gctgaagttt gatgatgcga cagggagaga
    26041 acaggtctac atccacgcgc agaagaacat ggataccgaa gtgctcaacg accgtaccac
    26101 caccgtaaaa cacgatcatc gcgaaaccgt aaaaaatgac cagacggtca cgatccagga
    26161 aggtaaccgc cttcttacgg tggaaaaagg ccacaagatc accggagtac tgaaagggtc
    26221 tttatctgag gatgtctttc aggacagagg cacgattgcc ggttcggtgc atgttgacgc
    26281 tgtaaacaat ggtggcgaag gcaacggtat acaggcttat acggcaatta aggaaattat
    26341 gctggccgtg gaggaaagca aaattgcgct gacgccggat ggcattcagc tacaggtcgg
    26401 ggaatcaacg gtaatcaggc tgtcgaagga tggcatcacc atcgtgggcg gttctgtttt
    26461 catcaactga ttcactcaga ttaacaggga gtattttatg cagttcactt ttaatgaagg
    26521 tcatattcaa ctgccatcgc aatggcagga tcagtcgatg caggtcctgg tatccacgga
    26581 taacagtggc atcaacctgg tcattacccg cgaacccgtt tcacagggaa ccctgacacc
    26641 agagctgtac caggaaacgc tggcgctgta ccagggcaaa cttgatgggt acaccgaaca
    26701 tgcctgccgg gaaatcactc tggcagaggc cccggcctgg ctgctggatt attcctggaa
    26761 cggtccggaa gatgaaggga atcaggggcg gattagccag atagccgtat tccagcggcg
    26821 tggagatacg ctgctgacct tcactttcag cacctcctta tccctgaaga acagtcaaaa
    26881 gacgatgctg ctggaggtga taaagagctt caccccgttg ccaccggaga atgacatcca
    26941 aaaggaccag ccgcgctgac ataaacgagg gataaaacat gagtgaagga ccaggcgggc
    27001 cacagggagc gaccgcaggc ggtacgctgg caatgcggat gctgtcgcag cgggcgatgg
    27061 ccgccagcca gatgaaacgg gcagccaacg acaaagccat tgcacagatg ttggcatcaa
    27121 aaaagtctgg cccccccgcc gccaggctgg gcgatgaaat tcagcacaag agttttttgg
    27181 gggcgctggc aggggccgtg ctgggggcga tagtgaccat agcagaaggt tgcctgatta
    27241 tggccgcctg tgccaccggc ccttatgcgc tggttctggt gcctgcgctg atgtatgcca
    27301 gctataaggc gagtgattat gtggaggaga aacagaacca gcttgaatca tggataaaca
    27361 gcttttgtga cacggacggc gccatcaata ccggttctaa aaatgtaaaa attaacggaa
    27421 agccagcagc ccgtgcagcc gtcacccttc cccctcctcc cccacctgga gcaatacctg
    27481 aagtcccaca gggggaaccc tcatggggtg atattgccac tgacctgctt gaatcggcag
    27541 cggaaaaagc agtaccactg gcgaaggcct gggggaacgc tgttatcacc ctgacggaca
    27601 gcaatgccgg ttttatggat cgcgtcagcg ccggcgcatc gcttctgttt cccgccggtc
    27661 cggtattaat ggagtttgcc accatggtgg gcgggcgtgg cgaaatcaaa aaagatgtgg
    27721 atttcccgga agccggtgag gacacggcgc tctgcgacaa ggagaacaaa ccaccgagga
    27781 tagcccaggg cagcagcaac gtctttatca acaatcagcc tgccgcgcgc aagggcgaca
    27841 aactggagtg cagcgcggca atcgtggaag gttcgccgga cgtctttatt gggggtgagc
    27901 aggtcaccta tctggatatc cagccggagt tcccgccatg gcagagaatg atcctgggag
    27961 gaataacgat agccagctat cttctgccgc cagcaggact gctgggaaaa ctggggaatc
    28021 tggcgaaact gggcaaactg ggaaacctgc tggggaaaag cggtaagctg ctgggcgcaa
    28081 agctcggcgc gttgctgggg aaaacaggta attcgttaaa aagtattgcc aataaagtca
    28141 tcagatgggt aacagatcct gtcgatccgg taaccggcgc atactgcgac gaacgtaccg
    28201 actttacgct gggccagaca ctccccctct ccttcacccg tttccacagt tctgtactgc
    28261 cgctgcatgg cctgacgggc gtgggctgga gcgactcctg gagcgaatac gcctgggtgc
    28321 gtgaacaggg aaaccgggtg gatgtcatca gcctgggagc cacgctgaac ttcgccttcg
    28381 acggtgaaag tgatacggcg gttaacccgt atcacgccca gtacattctg cgccgccgtg
    28441 atgattatct ggagctgttc gacagggatg cactgagcag ccgcttcttt tatgacgcct
    28501 ttccgggaat gcgtctgcgc cacccggtga ctgacgatac cagcgatgac cgcctggcac
    28561 acagccccgc agaccggatg tacatgctgg gcgggatgag cgacaccgcc agcaaccgca
    28621 tcacgtttga gcgcgacacc cagtaccgga tcacgggtgt cagtcacacc gacgggatcc
    28681 ggcttaaact gacgtaccac gccagcggct acctgaaagc cattcaccgc acggataacg
    28741 gcatacagac gctggcgacc tacgaacagg atgcgcggct ggactaccac ctgttttatg
    28801 agtacgacgc tgcggaccgg atcatccgct ggtccgataa cgaccagacg tggagccgtt
    28861 tcacctacga tgcacagggc cggtgcgtga ccgtcaccgg ggcggagggc tattacaacg
    28921 ccacgctgga ctatggtgac ggctgcacca ccgtgacgga cggcaagggc attcaccgtt
    28981 attactatga tcctgacggc aatattctgc gggaagaagc gccggacggc agcaccacca
    29041 cgtatgaatg ggatgaattc catcacctgc tggcccgcca ctcccctgcc gggcgggtgg
    29101 agaagtttga atacaacgcc gcacacggtc agttaagccg ttacacggcg gcagacggcg
    29161 cggattggca gtactgctat gatgagcgcg gcctgctcag caacatcacc gcccctgccg
    29221 ggcagacgtg gacgcagcag tgtgatgaac gcggcctgcc ggtgagtctg gtatcgccac
    29281 agggcgaaga gacccggctg gcgtacaccc ctcaggggct gctgtcgggg atattccgcc
    29341 aggatgaacg gcgtctgggc atagagtacg accaccacaa ctggccggaa acactcaccg
    29401 acgtgatggg ccacgaacac cacaccgaat acagcggtca cgacctgccg gtgaagatgc
    29461 gcggccccgg cggtcagtca gtgcggttgc agtggcagca gcaccataaa ctgagtggcc
    29521 ttgagcgggc aggaaccggc gcggaaggat tccgctacga ccgccacggc aacctgctgg
    29581 cgtacacgga cggtaacggc gttgtctgga caatggagta cggcccgttt gatttgccgg
    29641 tggcgcgaac ggacggtgaa ggccaccgct ggcagtaccg ctacgataaa gacacgctgc
    29701 aactgacaga agtcattaac ccgcagggcg agtcttatct ttatattctg gacaactgtg
    29761 gccgggtgac ggaagaacgt gactggggcg gcgtggtctg tcgttaccgt tatgacgctg
    29821 atggcctgtg taccgccagg gtcaacggcc tggaggaaac catcctctac agccgggatg
    29881 ccgcaggccg cctggcagaa gtcatcactc cggaaggcaa aacgcagtat gcgtatgaca
    29941 aatccggcag gctgacgggt atcttcagcc cggacggcac atcacagcgc accggctatg
    30001 atgaacgcgg gcgggtgaat gtcaccactc agggccgacg ggccattgaa taccactacc
    30061 ccgacgaaca caccgtcatc cgctgtatcc tgccaccgga agatgaacgc gacagacacc
    30121 ccgacggatc cctgctgaaa accacatacc gctacaacgc cgccggagaa ctgacggagg
    30181 ttatcctgcc gggggatgag acgctgacgt tcagccgtga tgaggcggga cgtgaagtgc
    30241 tccggcacag taaccggggt tttgcctgtg aacagggctg gaatgcagcc ggtcagcctg
    30301 tcagccagcg cgccggactt ttcccggcgg aagccacatg gggcggactg ctcccttcac
    30361 tgctacggga ataccgttac gacagcgcgg gtaacgtatc aggcgtcacc agccgggaag
    30421 attacggacg ggaaacacac cgggagtacc ggcttgaccg gaacggccag gtcacggcgg
    30481 tgacagcctc aggcaccggg ctgggctatg gcgaaggcga cgagacttat ggctatgaca
    30541 gctgcggcta cctgaaggcg cagtctgcgg gcagacaccg gataagcgga gagactgacc
    30601 agtatgccgc aggccaccgg ctgaaacagg ccggaaacac acagtatgac tatgacgccg
    30661 caggccggat ggtcagccgc acaaaacacc gtgacggcta ccgcccagaa acagagcggt
    30721 tccggtggga cagccgggac cagctgaccg ggtatcgcag cgcacagggg gagcagtggg
    30781 aataccgcca cgacgccagc ggcagacgga cggaaaaacg ctgcgaccgg aagaaaatcc
    30841 gttttacgta cctgtgggac ggcgacagta ttgcggaaat ccgggaatac cgcgatgata
    30901 aactgtacag cgtaaggcac ctggtgttta acggctttga gctgataagc cagcagttca
    30961 gccgggtacg acagccgcat ccgtccgtgg ccccgcagtg ggtgacgcga acgaatcatg
    31021 cggtgagcga cctgacgggc cgcccgctga tgctctttaa cagtgaaggt aaaaccgtct
    31081 ggcggccggg gcagaccagc ctgtgggggc tggcactcag cctgcccgca gacacagact
    31141 acccggaccc gcgcggggag cgggacccgg aagcggaccc cggcctgctg tatgcgggac
    31201 agtggcagga tgcagaatcg gggctgtgct ataaccggtt ccggtactac gagccggaaa
    31261 ccgggatgta cctggtgagt gatccgctgg ggttgcaggg aggggagcag acttatcggt
    31321 atgtgccgaa tccttgtggg tatatcgatc ctttggggct ggctatatgt cagttagccc
    31381 gctggacgaa atgggggagt gagcaaagca acatatctga tgttttgaac tcattaggga
    31441 atagagcact taaatatgct aatggtgatt ggataaaatc agaggctgca ttcaataaat
    31501 acataaacat gataaataaa agactagaat taac
//
LOCUS       DAZFBR010000051        31192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFBR010000051 DAZFBR010000000
VERSION     DAZFBR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.051"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1607
                     /locus_tag="ACHJZ2_002517"
     CDS             <1..1607
                     /locus_tag="ACHJZ2_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO4568604.1"
                     /translation="DSVDTEYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFA
                     FIIIVLLVVYLMTTRVFRRQFSEMTELINTLAFLPDSTDQIEALKIREGDAKEIISIK
                     NSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVE
                     AVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDF
                     DSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEI
                     EENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFIT
                     IAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTT
                     IMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGLRISIDDFGTGLSNLKRFY
                     EINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGC
                     VQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            1615..2943
                     /locus_tag="ACHJZ2_002518"
     CDS             1615..2943
                     /locus_tag="ACHJZ2_002518"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4568605.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(3013..3342)
                     /locus_tag="ACHJZ2_002519"
     CDS             complement(3013..3342)
                     /locus_tag="ACHJZ2_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4568606.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(3332..3718)
                     /locus_tag="ACHJZ2_002520"
     CDS             complement(3332..3718)
                     /locus_tag="ACHJZ2_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4568607.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGSKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            complement(3944..5269)
                     /gene="rimO"
                     /locus_tag="ACHJZ2_002521"
     CDS             complement(3944..5269)
                     /gene="rimO"
                     /locus_tag="ACHJZ2_002521"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO4568608.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            5482..5865
                     /gene="bssR"
                     /locus_tag="ACHJZ2_002522"
     CDS             5482..5865
                     /gene="bssR"
                     /locus_tag="ACHJZ2_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO4568609.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFLKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            5976..7091
                     /gene="yliI"
                     /locus_tag="ACHJZ2_002523"
     CDS             5976..7091
                     /gene="yliI"
                     /locus_tag="ACHJZ2_002523"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO4568610.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(7088..7714)
                     /gene="gstB"
                     /locus_tag="ACHJZ2_002524"
     CDS             complement(7088..7714)
                     /gene="gstB"
                     /locus_tag="ACHJZ2_002524"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO4568611.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            7960..9162
                     /gene="dacC"
                     /locus_tag="ACHJZ2_002525"
     CDS             7960..9162
                     /gene="dacC"
                     /locus_tag="ACHJZ2_002525"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4568612.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(9209..9967)
                     /gene="deoR"
                     /locus_tag="ACHJZ2_002526"
     CDS             complement(9209..9967)
                     /gene="deoR"
                     /locus_tag="ACHJZ2_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO4568613.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(10025..10621)
                     /gene="ybjG"
                     /locus_tag="ACHJZ2_002527"
     CDS             complement(10025..10621)
                     /gene="ybjG"
                     /locus_tag="ACHJZ2_002527"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO4568614.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            10906..12143
                     /gene="mdfA"
                     /locus_tag="ACHJZ2_002528"
                     /pseudo
     CDS             10906..12143
                     /gene="mdfA"
                     /locus_tag="ACHJZ2_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(12184..12468)
                     /gene="ybjH"
                     /locus_tag="ACHJZ2_002529"
     CDS             complement(12184..12468)
                     /gene="ybjH"
                     /locus_tag="ACHJZ2_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO4568615.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(12554..13369)
                     /gene="ybjI"
                     /locus_tag="ACHJZ2_002530"
     CDS             complement(12554..13369)
                     /gene="ybjI"
                     /locus_tag="ACHJZ2_002530"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO4568616.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(13369..14577)
                     /locus_tag="ACHJZ2_002531"
     CDS             complement(13369..14577)
                     /locus_tag="ACHJZ2_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4568617.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14661..15197
                     /gene="rcdA"
                     /locus_tag="ACHJZ2_002532"
     CDS             14661..15197
                     /gene="rcdA"
                     /locus_tag="ACHJZ2_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO4568618.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(15372..17057)
                     /locus_tag="ACHJZ2_002533"
     CDS             complement(15372..17057)
                     /locus_tag="ACHJZ2_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO4568619.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            17327..17704
                     /locus_tag="ACHJZ2_002534"
     CDS             17327..17704
                     /locus_tag="ACHJZ2_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO4568620.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(17734..17991)
                     /gene="grxA"
                     /locus_tag="ACHJZ2_002535"
     CDS             complement(17734..17991)
                     /gene="grxA"
                     /locus_tag="ACHJZ2_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO4568621.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            18151..18438
                     /locus_tag="ACHJZ2_002536"
     CDS             18151..18438
                     /locus_tag="ACHJZ2_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO4568622.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            18422..19144
                     /gene="nfsA"
                     /locus_tag="ACHJZ2_002537"
     CDS             18422..19144
                     /gene="nfsA"
                     /locus_tag="ACHJZ2_002537"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO4568623.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            19205..20107
                     /gene="rimK"
                     /locus_tag="ACHJZ2_002538"
     CDS             19205..20107
                     /gene="rimK"
                     /locus_tag="ACHJZ2_002538"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO4568624.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            20195..20671
                     /locus_tag="ACHJZ2_002539"
     CDS             20195..20671
                     /locus_tag="ACHJZ2_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO4568625.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            21022..22134
                     /gene="potF"
                     /locus_tag="ACHJZ2_002540"
     CDS             21022..22134
                     /gene="potF"
                     /locus_tag="ACHJZ2_002540"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO4568626.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            22229..23362
                     /gene="potG"
                     /locus_tag="ACHJZ2_002541"
     CDS             22229..23362
                     /gene="potG"
                     /locus_tag="ACHJZ2_002541"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO4568627.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            23372..24325
                     /gene="potH"
                     /locus_tag="ACHJZ2_002542"
     CDS             23372..24325
                     /gene="potH"
                     /locus_tag="ACHJZ2_002542"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO4568628.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            24322..25167
                     /gene="potI"
                     /locus_tag="ACHJZ2_002543"
     CDS             24322..25167
                     /gene="potI"
                     /locus_tag="ACHJZ2_002543"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO4568629.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            25227..25715
                     /locus_tag="ACHJZ2_002544"
     CDS             25227..25715
                     /locus_tag="ACHJZ2_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO4568630.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            25756..26883
                     /gene="rlmC"
                     /locus_tag="ACHJZ2_002545"
     CDS             25756..26883
                     /gene="rlmC"
                     /locus_tag="ACHJZ2_002545"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO4568631.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            27163..28506
                     /locus_tag="ACHJZ2_002546"
     CDS             27163..28506
                     /locus_tag="ACHJZ2_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO4568632.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            28528..28848
                     /locus_tag="ACHJZ2_002547"
     CDS             28528..28848
                     /locus_tag="ACHJZ2_002547"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4568633.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            28860..30341
                     /locus_tag="ACHJZ2_002548"
     CDS             28860..30341
                     /locus_tag="ACHJZ2_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO4568634.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(30392..31123)
                     /gene="artJ"
                     /locus_tag="ACHJZ2_002549"
     CDS             complement(30392..31123)
                     /gene="artJ"
                     /locus_tag="ACHJZ2_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO4568635.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
BASE COUNT         7672 a         7386 c         8016 g         8118 t
ORIGIN      
        1 ttgattctgt cgatactgaa tatatcgaaa aaacatcagc ggtgccaaat aacgcattat
       61 tcgaaatcta tttttattat ccgggcggta atttattgaa cgcatcagat aaactttttt
      121 atctgccgtt tgcgttcatt attatcgtat tgctggtggt ttatttaatg accactcgtg
      181 tgttccgtcg gcaattttct gaaatgacag agctgattaa tacgctggcg tttttgcctg
      241 actcaacgga tcagatcgag gctctgaaaa ttcgcgaagg cgatgcgaaa gagattatca
      301 gcatcaaaaa ttcgatcgcg gaaatgaaag atgccgaaat tgaacggtca aataaattgc
      361 tctcactgat ctcttacgat caggaaagtg gttttattaa aaatatggcg attattgagt
      421 ccaacaataa tcagtatctg gctgtgggga tcatcaaact gtgtggtctg gaagccgtgg
      481 aagctgtgtt tggtgttgat gaacgcaata aaatcgtcag gaaattgtgt cagcgaattg
      541 ctgagaaata tgcacaatgc tgcgatatcg tgacattcaa tgccgatctc tatttacttc
      601 tgtgtcggga aaatgtacag acatttaccc gtaaaatagc gatggtaaac gattttgaca
      661 gcagctttgg ctaccgcaat ctgcgcatcc ataagtctgc catttgtgaa cctttgcagg
      721 gggaaaacgc ctggagttac gcagaaaaac tgaaactggc gatttccagt atccgtgacc
      781 atatgttctc cgagtttatt ttctgtgatg acgcgaaact caacgaaata gaagagaata
      841 tctggattgc gcgtaatatt cgccatgcaa tggaaattgg cgaactattc ctcgtctatc
      901 aaccgatcgt tgatattaac acccgcgcca ttctgggcgc ggaggcgttg tgccgttggg
      961 tgtctgcgga gcgggggatc atttcaccgc tgaagttcat taccattgct gaagatatcg
     1021 ggtttatcaa tgagctgggt tatcagatta ttaaaacggc aatgggtgaa ttcagacatt
     1081 ttagtcagcg tgcatcgctg aaggatgatt tcttactgca tattaatgtt tcgccctggc
     1141 agttaaacga accgcacttt catgagcgtt ttaccaccat catgaaagaa aatggcctga
     1201 aggcgaacag cctctgtgtt gagatcactg aaaccgtgat cgagcgaatt aatgaacatt
     1261 tttatctcaa tattgaacaa ctgcgtaaac aagggttacg gatatcgatt gatgactttg
     1321 gcaccggttt gtcaaacctg aaacgttttt atgaaattaa tccagacagc ataaaggtgg
     1381 actcgcaatt taccggcgat attttcggta ctgcgggaaa aattgtgcgc attattttcg
     1441 acctggcacg ctataaccgg atcccggtga ttgcggaagg cgtagagagc gaagacgttg
     1501 cgcgcgaatt aatcaaatta ggatgtgttc aggctcaggg gtatctgtat cagaaaccca
     1561 tgccattctc cgcctgggat aaaagtggaa aattagtaaa agagtagttt acgtatgtcc
     1621 agaatcaata agttcgtact tacagtcagt ctgctgattt ttatcatgat ttcagcagtt
     1681 gcctgcggga tctacactca aatggtaaag gaacgggtgt atagcctgaa acagtccgtt
     1741 attgatactg cttttgcggt ggcaaatatt gctgaatatc ggcgtagcgt ggcaattgat
     1801 cttatcaaca cgctaaatcc cacggaggaa cagctgttgg ttggcttacg cacagcttac
     1861 gccgactcgg tttcaccctc ttatttgtac gatgtcggtc cttatctgat ttccagtgac
     1921 gaatgtattc aggtaaagga gttcgagaaa aattattgtg cagatattat gcaggttgtg
     1981 aagtatcgac atgtcaaaaa tacagggttt atctcttttg acggtaaaac cttcgtctat
     2041 tacctctatc cggtaactca caatcgtagt ctgatatttt tgcttggtct ggagcgtttt
     2101 tctttactgt caaaatcgct ggcgatggac agcgagaacc tgatgttctc tctgtttaag
     2161 aacggtaaac cggtgaccgg tgatgaatat aatgctaaaa acgccatctt caccgtttcg
     2221 gaagcgatgg agcacttcgc ctatttgccg accggattgt acgtatttgc gtataaaaaa
     2281 gatgtttatt tgcgggtctg tacattgatt attttctttg ccgcattggt ggcagtgata
     2341 tcgggtgcca gttgcctcta tctggtacgc agagtgatta atcgtggtat tgtggagaaa
     2401 gaagccatta ttaataacca ttttgaacgc gtactggatg gcgggctttt cttttcggct
     2461 gccgatgtca aaaaactcta cagtatgtat aactcggcgt tcctggacga cctgaccaaa
     2521 gcaatgggta gaaaatcctt tgacgaagat ttaaaagcgc tgccggaaaa aggcggttat
     2581 ttgtgcctgt ttgacgtcga taaattcaaa aatattaacg acaccttcgg tcatttgctg
     2641 ggcgatgaag tgttgatgaa agtggtgaaa atccttaaat cacagatccc ggtagataaa
     2701 ggtaaagtct accgcttcgg cggtgacgaa tttgcggtga tttatactgg cggcacgctg
     2761 gaagagttac tatcgattct aaaagaaatc gttcatttcc aggtgggaag cattaattta
     2821 agcaccagta tcggtgtagc gcattcaaat gaatgtacta ccgtcgaacg cttgaaaatg
     2881 ctggcggatg agcggctgta taagagtaag aaaaacggca gggctcagat tagctggcag
     2941 tagaattaga atagtgttag tacccctgaa tcaaggggta ctaatgaatg tggtaaatca
     3001 tttgcaaaca ttttactttg caaaaacttc cggccctttt attgccaatg tattcaaaat
     3061 acgtagtgcc gggccgctcg gttttatttc attacgttcc catttcgaga cactttcttt
     3121 tgacattccc attgtatgtg ccagcacgga ttgtgacatg ccgtatcggg tgcggatggc
     3181 tttgatctct tcacccgtca ttgatttgac tttggctata cgggcgctga cttcacgggc
     3241 tttgaccatg acggcaatgg catcaacctc actatcagac atcaggccca gtgcattcag
     3301 ttcctttgca tcttccagca tcatttgtaa tttatcgctc atttttcacc tcaatcaaga
     3361 agccagtttc gatcaaatcc tctatttgct tattgctaaa ggccagaaat gcatttgcca
     3421 tttttttgta ggctgccagt tcatcatctt cgatttcttt gcttccttta ctgcttagac
     3481 cactcttgga ccaaccatca taaaaaaaca catgatttgc tgatttaaaa aagataattg
     3541 ttctggctcc accactcttt ccttgttgga ggggtaaccg ttttttgatg acaccgctac
     3601 ctaaatcagc ctcccatatg ccttgcatca cttcttcagc ggcagccatg agaactttat
     3661 cgtgaatacc aagttttttc gtgctttggc tgaatcgcct tgccttatac acacccatac
     3721 atccatcctt gtggtaatgt ttactatagt aaccattacg cttatttcaa gtaattgcga
     3781 tcaaaacttt aatttggtgt gaaaaagtga gagttgcctc gagtacgatt catttttctc
     3841 gatttatgcg tcaggggatt ttgtgcgtaa gggtaggaca tcgggaaggg tagtagaggc
     3901 agatcgaaga acaatatcct tgttagatct gcctttagat taattaaacc cggctacccc
     3961 acaaatcgta ctcatcggcg tgctcgactt tcacacgcag gatatcaccc ggcttaacgt
     4021 tggtttcacc attgagataa accgcaccgt cgatttccgg tgcatctgcc atgctgcgac
     4081 caatcgcgcc ttcttcgtcc acttcgtcga taatcaccag aatttcacgg cccactttct
     4141 cttgcaggcg ctcggcggaa atctgctgct gcaactgcat gaaacggttc cagcgttctt
     4201 ctttcacttc ttccggaacc tggtcaggca gggcattggc gtctgcacct tcaaccgggc
     4261 tgtatttaaa gcagccaacg cgatccagac gcgcttcttt caggaagtcg agtagcatct
     4321 ggaaatcttc ttccgtctcg ccagggaagc cgacaataaa ggttgagcgt agggtcagtt
     4381 ccgggcagat ttcgcgccac tgtttgatgc gcgccagttg gcgatctaca gaacccggac
     4441 gcttcatcag tttgagaatg cgcgggctgg cgtgctgcaa cggaatgtcc agatacggca
     4501 ggattttgcc ttctgccatc agtgggatga cgtcgtccac atgcggataa gggtaaacgt
     4561 agtgcagacg tgtccagatc cccagtttcg ataactgttc gcacaggctg accatgctgg
     4621 tttttaccgg ctcgccgttg tggaagccag tacgatgttt aacatcaacg ccataggcgg
     4681 aagtatcctg cgagatcacc agaatctctt taacgcccgc atctaccaga cgtttcgctt
     4741 cacttaatac ttcgccaatc ggacggctca ccaggtcgcc gcgcatagac ggaataatgc
     4801 agaaggtgca gcggtgatta cagccttcag aaattttcag ataggcataa tgacgcggcg
     4861 tcagtttgac accttgttct ggcaccaggc tcaggaatgg gttgtgtttc ggttttggca
     4921 cgtagtgatg aacgtgctcc agaacctgct catagctatg aggcccggtg atttccagca
     4981 ctttcgggtg gacttcgcgg atctgatctt cttttgcccc cagacaaccg gtcacaatta
     5041 ccttgccgtt ttcattcaac gcttcaccaa tggcttccag tgattcttgt accgcgctgt
     5101 caataaagcc gcaggtgttg acgatcacca tgtccgcatc gtcatagctc ggtaccacgt
     5161 cataaccttc agtgcggagt tcggtgagaa tacgctctga atcgacaagg tttttcggac
     5221 agccaaggga aacaaagccg attttcggct ggggagttac tttgctcata gcttaaaaaa
     5281 tattcagtta caggaaaggt cagggcaggg attctacaga gttctggata aaatttgtat
     5341 cgcaatctca ttcgctggcg gaggcgaagg aaatgtaaat tttgttaatt cggcgtgaag
     5401 aattgatcct ggacagcatt atgctcaaaa aatagccata ctatttaatt gcaacaaggc
     5461 tggaaagagg aggatcgaag tatgttcgtt gacagacagc gaatcgatct gctgaaccgg
     5521 ttgatcgacg cacgcgttga cctcgccgca tacgtgcaac tgaggaaggc aaaaggatac
     5581 atgtccgtca gcgaaagcaa tcatctacga gataactttt taaaactgaa tcgcgaactg
     5641 cacgataaat cgctgcggtt gaatcttcat ctggatcagg aagagtggag tgctcttcat
     5701 catgctgaag aagcattagc gacagccgca gtatgtttga tgagtgggca ccatgattgc
     5761 ccgactgtta ttaccgtcaa cgccgataag cttgaaaatt gtctgatgag cttaacgctg
     5821 agtatccaga gcctgcagaa gcacgccatg cttgagaagg cctgaaaact aagggggaga
     5881 aagcgtctcc cccttcatgt ttaagttttt gtaaaaatga atttgttatc tcctccactg
     5941 actacgcttt aagccagagt caatccggag gcgttatgca tcgacaattc tttttccttg
     6001 tgccccttat ttgtctttct tccgctctct gggcggctcc tgcaacggta aatgtcgaag
     6061 tactgcaaga caaactcgac catccctggg cactggcctt tttacccgat aaccacggta
     6121 tgttaatcac tctgcgcggc ggcgagttgc gtcactggca agcaggaaaa ggattatctg
     6181 cgccgctttc cggagttccg gacgtttggg cgcacgggca gggcggcctg ctggacgtgg
     6241 ttttagcgcc tgattttgct cagtctcgcc gcatctggtt aagttattcc gaagttggcg
     6301 atgatggcaa agccggaact gctgtggggt atggccgctt aagtgatgat ctctcaaaag
     6361 tgaccgactt tcgcaccgtc tttcgccaga tgccaaaact gtctaccggc aaccattttg
     6421 gcgggcggct ggtattcgac ggtaaaggtt atctttttat tgctctgggc gaaaacaatc
     6481 agcgcccgac ggcgcaggat ctggataaat tacagggcaa actggtgcgt ctgaccgacc
     6541 agggcgaaat cccggatgat aatcctttta taaaggaatc cggtgcgcgc gccgagatct
     6601 ggtcttatgg cattcgtaat ccgcaaggaa tggcgatgaa tccgtggagt aatgcactgt
     6661 ggctgaatga acatggcccg cgcggtggtg atgaaattaa tatcccgcaa aaaggcaaaa
     6721 actacggctg gccgctggca acctggggaa tcaactattc aggctttaag ataccggaag
     6781 cgaaagggga gatcgtcgcc gggaccgagc aacctgtttt ttactggaaa gattcgcccg
     6841 ccgtgagcgg catggccttc tataacagcg ataaattccc ccagtggcag caaaaattat
     6901 ttattggcgc gctgaaagat aaagatgtca ttgtgatgag cgtcaacggc gacaaagtaa
     6961 cagaagatgg ccgtatttta acggacagag ggcagcgaat tcgtgatgtt cgcactggac
     7021 ccgacggtta tttatacgtt ctcaccgacg agtccagtgg ggaattactt aaagttagcc
     7081 cacgcaatta gctaacggga atcatcacca ctttgcgcac tgcggggcgt tcagtgagtt
     7141 gctgatacca gcgttgcaga tttggacgcg gtgtccaggt caggccaaca ttaaacaaat
     7201 tgtagatgaa cggtgcgata gcgatatcgc ccacaccaaa ctcgtcgcca gagaaccatt
     7261 ttacttttgc cagttccgca tcgagcaggg caaacagggc gtcgcactct ttgcaactgg
     7321 catcaatggc ggcctgatcg cgctcttccg gtggtgttct gactaatccc atcaggatcc
     7381 cgcgatgagc attactgagt gtctggtttg cccagtccat ccatttttct gcttccgcac
     7441 gacgtgccgg tgagtcgatc cacaggcgtt tttgcccgta ctgtgccgcc agatagcgga
     7501 caatggcgtt tgattcccaa agaatgagat cactttcgtc gtcacgcaac aacggcacca
     7561 gcccgttagg gttcatcgcc agaaaatcag catcgtgatt tatcccaaac tcacggcccg
     7621 cgagaatttg ctcataaggt agttccagtt cttcgagcgt cagcaatact ttttttacgt
     7681 tggttgaatt attccgaccc cacagcgtaa tcatactcac cccttttccg acaggcagcc
     7741 acaggctgaa caggactccg atggtgaagt aaagttaact tttaagcaac agctggcaaa
     7801 aaaatgacac cagaagcagc gaagcggcag gatattgcat aaactttaaa aactttacca
     7861 acttacggtt tctttaagtt tgtgtgtgcg ttattaatca ccgaacttat catacggcga
     7921 tataacgtat ttttttgaat ggatactcgg gtggcattta tgacgcaata ctcctctctc
     7981 cttcgtggtc ttgcagcggg ttctgcattt ttattccttt ttgccccaac ggcattcgcg
     8041 gccgaacaaa ccgttgaagc gccgagcgtg gatgcgcgtg catggatttt aatggattac
     8101 gccagcggta aagtgctggc agaaggcaat gcggatgaga aactggatcc cgcgagcctg
     8161 actaaaatca tgaccagcta tgtggttggg caggcgctta aggccgataa gattaaactc
     8221 accgatatgg tgacggtcgg taaagatgcc tgggcgacgg gaaatccggc actgcgtggt
     8281 tcatcggtaa tgttcctcaa accgggcgat caggtttcgg tggcagactt gaacaaaggt
     8341 gtgattatcc agtccggtaa tgacgcctgt attgcgctgg ccgattacgt tgccgggagc
     8401 caggagtcat ttattggttt gatgaatggt tatgccaaaa aactggggct gaccaacact
     8461 accttccaga cggtgcacgg tctggatgcg ccggggcagt tcagtaccgc gcgcgatatg
     8521 gcattgctgg gtaaagcatt gatccacgat gtgccggaag agtacgccat tcataaagag
     8581 aaagagttca ccttcaacaa aattcgtcag cctaaccgta accgtctgct gtggagcagc
     8641 aatctgaatg ttgatggcat gaagacagga accacggcag gcgcgggata taatctggtt
     8701 gcttcggcta cccagggcga tatgcgttta atctccgtag tgctgggggc gaaaaccgac
     8761 cgtatccgtt ttaatgagtc tgagaaatta ttgacctggg gtttccgctt ctttgaaacc
     8821 gtgacgccaa ttaaacctga tgccaccttt gtgactcagc gcgtctggtt tggtgataag
     8881 agcgaagtga atctcggggc aggcgaagcg ggctcagtga ccataccgcg tgggcagctg
     8941 aaaaacctga aggcgagcta cacgttaacg gaaccgcagc ttaccgcacc gctgaaaaaa
     9001 ggtcaggttg tcgggaccat tgatttccag cttaacggta aatccattga gcagcgtccg
     9061 ctgatcgtga tggaaaatgt ggaagagggc ggattctttg gtcggatgtg ggatttcgtg
     9121 atgatgaaat tccatcagtg gttcggcagc tggttctctt aatcttctga taaccggatg
     9181 gcgcgaaacg tcatccggtt atacgtcatt aatacatcaa cttaatacgc tgcgtctgcg
     9241 cgtacttcac atactcatct tccgggcaac aatcgctcac cacaatatca aagcgtttca
     9301 ggtcacccat gcgcgccgga cgcaccttgc caaatttact gtggtcaaca accagcacat
     9361 gcttttgcgc catcgacatg gcccagtgtt ttaccggcaa ctcttcaaga ttaaaacagg
     9421 tagcgccttt actgacatgc acgcccgccg cagaataaaa agcgatatcc gggcaaaaat
     9481 tattcagcgt ttgctgaaaa tcgatgggtt tgaaaatggc gttgctggcg tgaaattcac
     9541 caccgcacag aaatgcgcgg caatggggtt tttctttcag cgccagaaag gtatttagcg
     9601 aataacaaac ggcggtaaaa gggatttcat tatcaatcgc ttcaataatc cacggcgtgg
     9661 tggtgccaca gtcaaaaaag agggtctgat cgggttctac cagtgtcgca gccagttttg
     9721 ccgcccggcg tttttcttcc accaggcggg atttttgatc gcttaacagg taatggctgg
     9781 cgctgcgcgg ttccagaaca atatagccgc cgagcaaaac gacgggcgca ctgtggttgt
     9841 tcagatcgcg acgaatcgtc atctccgaaa ccccaagcag ggcggcggcg tctttaagat
     9901 gtaacttatc gctgcgtttt aattcttgca gcagctgccc gatacgctct tcgcgacgtg
     9961 tttccataat ccctctgaat agttattgaa gcgagccgct caatactaca ctttttagca
    10021 gagatcagtc acgcacccag cctttgcgga tcggtaatgc aaaacagaag cgataccacg
    10081 attgcagacg ttgatagagt ttatgcccca tcgcttgcca gataatctgg gcactaaggc
    10141 agccaatcat acctgccagc aatccaccga gcatatccag cggccagtgg acgccaagat
    10201 aaacgcgcga ccaggcaatg acgacggcca gcaccattaa aagtgagccg gaccacaggc
    10261 gatgccagca taaaaatgcc agtgcaaagg tgaaaatcac cgtaccgtga tcgctcggga
    10321 atgagtcatc cgccgcatga tgcaggaagt tatagccgat attttcgaca aacggtcggt
    10381 cgtgcggaaa aagatgtccc atcgtccagg acaccaacag gctgaccgcc agcgcgatag
    10441 cgatttttat caccagttgc cgttgtgctg taagccccca caaccaaagt accgcggcca
    10501 gcagtggcac cacggtaatc aaatctttag caataaaaat cgccaacgag atcatccacg
    10561 gagccgagtc tggcgtcgcg ttaataagag agaatagaga gagattcaaa ttttccagca
    10621 taacttcccg acgcaaagtg attaaaaggg agccaataca ggcaagtcgt tgagaataaa
    10681 gtgcaggtta aactgggtaa agcggcatcg tcttatttcc ctcaagcggc ctgtttacgg
    10741 tgggtgattg taacgggcat aggttaaata aaacttaaag aaagcgtagc tatactcgca
    10801 ataatgtaag aatgtgctta accgtggttt cagctacaaa attcgctttc tcgttagctg
    10861 cgcttttatt aaactctgcg cgattattat tggcgaagaa attgcatgca aaataaatta
    10921 gcttccggtg ccaggcttgg acgtcaggcg ttacttttcc ctctctgtct ggtgctttac
    10981 gaattttcaa cctatatcgg caacgatatg attcaacccg gtatgttggc cgtggtggaa
    11041 caatatcagg cgggcattga ttgggttcct acttcgatga ccgcgtatct ggcgggcggg
    11101 atgtttttac aatggctcct ggggccgttg tcggatcgta ttggtcgccg tccggtgatg
    11161 ctggcgggtg tggtgtggtg tggtttattg tcacctgtct ggcaatatta ctggcgcaaa
    11221 acattgaaca attcaccctg ttgcgcttct tgcagggcat aagcctctgt ttcattggcg
    11281 ctgtgggata cgccgcaatt caggaatcct tcgaagaggc ggtttgtatc aagatcaccg
    11341 cgctgatggc gaacgtggcg ctgattgctc cgctacttgg tccactggtg ggcgcggcgt
    11401 ggatccatgt gctgccctgg gaagggatgt ttgtcttgtt tgccgcattg gcagcgatct
    11461 cctttttcgg tctgcaacga gccatgcctg aaaccgccac gcgtataggc gagaaactgt
    11521 cgctgaaaga actcggtcgt gactataagc tggtgctgaa gaacggtcgc tttgtggcgg
    11581 gggcgctggc gctgggattc gttagcctgc cgttgctggc gtggatcgct cagtcgccga
    11641 ttatcatcat taccggcgag cagttgagca gctatgaata tggtttgctg caagtgccta
    11701 ttttcggggc gttaattgcg ggtaacttgc tgttagcgcg tctgacctcg cgccgcaccg
    11761 tacgttcgct gattattatg ggcggctggc cgattatgat tggtctgttg gtcgctgctg
    11821 cggcaacggt tatctcatcg cacgcgtatt tatggatgac cgccgggtta agtatttatg
    11881 ctttcggtat tggtctggcg aatgcgggac tggtgcgatt aaccctgttt gccagcgata
    11941 tgagtaaagg tacggtttct gcggcgatgg gaatgctgca aatgctgatc tttaccgtcg
    12001 gtattgaaat cagcaaacat gcctggctga acgggggcaa cggactgttt aatctcttca
    12061 accttgtcaa cggaattttg tggctgtcgc tgatggttat ctttttaaaa gataaacaga
    12121 tgggaaattc tcacgaaggg taaaaaaatg cctgattgct ttgtgcgatc aggcattctc
    12181 gaattaatgg tgatggtcgt caatctggtg ttcgataacc atcccttcac ctacgctggc
    12241 aagatggcga acataaggat gcgggtggta agccggagct ggcgcaggag ccacatagac
    12301 ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg ctgactgaat caggaaccac
    12361 aaccactttg tatccactcg gcacatcgac ggtgacactt tgcgccatcg ccacaccagt
    12421 aaagcccatt aaaagcgcgc ccaacagtag acaatttttc ataatcattt cgcctgaaat
    12481 aatgcagcgg gttaatttcc gcgaattatg cagccttcat tcaggtattg tgttgccggg
    12541 cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt aagaacttta tcgatcacat
    12601 ccagtacgcc ttcacggtta ttggagcctg cccggtattt tgccgctgcg acgaccgcgt
    12661 tgccggcatt ttccattgca aaactaaagc ctgcctgacg cagcatctca atatcgttac
    12721 cgccatcgcc aaagaccacc acttcgctgt cgtctattcc ccataatttc tgcagttggc
    12781 gaaggccatt ggctttatgt acgccgggga taatcagatc gatgctgccg ttgccggtgt
    12841 ggaccggcac cataatatcg ccgatggcct catgtaatgt tttttgtact tgtggaatca
    12901 gttcatcgga aagattcagg ccaaacttaa agaagatatc ttctaagttg tcaaagttat
    12961 cgacgtattc cagacggtga taatacattt ccgccaccat tttcatggca tcgtcatatt
    13021 ttttgagcgt ataggcgcta ttttttccgc aggcaataat ttccacttcc gggcgcgtca
    13081 gcaaatgttc cacgacagta gtaaacgcat ccttcgacag ctcgccatta aaaacatctt
    13141 tgccttcact cactacccag ccgccgtttt cagccacaaa ggcaatttca ttagcaattt
    13201 cagggaagaa agagatcaac tgataatatt gattcccgct ggcgaccaca aagcgaatgc
    13261 cttgtgcttt catttgctga tactgagcca taaaccgctc acggttatag gttttttgat
    13321 cgcttaagaa agtaccatcc atgtctaccg caattaattt aatgctcatc aactattctc
    13381 catcgccgtc tgcgttttgg tatcgggttt ggcgacggct ttcgcgacaa tagccgcgag
    13441 aataaccagc gccagtacaa ccagcattgc actacgtaat ccataatgtt cgccgagata
    13501 gcccagcagc ggcggcccga cgaggaaagc cagataaccg gtcgttgcta ccacactgac
    13561 gcgggttggt gcatcggggc cggtatcgct ggcggcagaa atggtcagcg ggaagcccag
    13621 cgaggcaccc agtccccaga gtacaacaga caccccagcg acccaggcgc tatcgacaaa
    13681 aataatcagc ccaataccca acgcccccat tagcgcactg gcccgaacca cggcaacgcg
    13741 actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca acggtcatcc ccagggtaaa
    13801 accggcataa atcagcgagc cggaagtggg gctaaaaccg tgaccgtcaa ccattaataa
    13861 gggtaaccag tcgttggcag aaccttcggc aaaggccatc gccagcacca caacaccaat
    13921 cagcagcaac tggatatcgc gataaaaagg tacgcctttt tcgccatgct gggtgccatc
    13981 ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg gcgatatata taggcgcgat
    14041 gcctaccagc gccgccaata aaatgtgcac cgttgccgga acgccaaagg ccgtcagtgc
    14101 catcccgaca ccagcgcctg ccagcgtgcc caggctataa aaaccgtgca tcatcggcaa
    14161 aaccgtttta ttcatttctc gctcaacggc ggcaccttca acgtttatcg ccacttccgc
    14221 agaaccaaaa cttgccccaa agacgccgag accaacggca aagagcaggg gcgatgtcag
    14281 ccagagtgcc agacttaata tcatcatccc gaccaatgcg caggacatgg tgaccaggat
    14341 gacattacgt gtcccaaagc gtttcactaa ccacgccgag cagagaatac cgctcatcga
    14401 accgatcgac agaccaaaga gaacaccgcc catttcagcg atcgagacag agagaatatc
    14461 gcggatagca ggcgtacggg ttgcccagga cgccattaac aggcccggca aaaagaagaa
    14521 cataaacagc gcccaggttc ggcgtttcaa tgcattacgt gaagaattta cggtcataga
    14581 tcacgtcaaa ataagaagag ggaagacaac attagcaagg ttgtgtacat ttgtacacaa
    14641 ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc gcaacggcga gaaaaaatta
    14701 tccaggccac gctggaggcg gtgaaacttt acggaataca tgctgttacg caccgcaaaa
    14761 ttgctaccct tgccggggta ccgttgggat cgatgaccta ctatttttca ggaattgatg
    14821 agttgttgct ggaggcgttc agcagtttta ctgagatcat gtcccggcaa tatcaggcat
    14881 tttttagcga tgttagtgat gctcaaggcg catgccaggc tatcaccgat atgatctaca
    14941 gctcacaggt tgcaacgccg gataacatgg agctgatgta ccagctctac gcgctggcta
    15001 gccgaaaacc gctattaaaa acggtaatgc aaaactggat gcagcgcagt cagcaaacgc
    15061 tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga tgcgtttatt gaggggatga
    15121 cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga ggagattttg aggatggttg
    15181 agagggttgc agggtagtag ataaatttca ggcaacaaaa aacccatcaa ccttgaaccg
    15241 aaatggcggg gttgatgggc tccacaaaat ggggacatca aagaaaagca gtggcactaa
    15301 ttaagactga tgccctgcgg aaaagttctg cggttgtgca aaaaaatttc attttcaggg
    15361 caacttcagt tttatcctaa tcctggccat accatgacga tgattgtccc tgccagcgtc
    15421 agcaggacgt tggcgattgc gtaggtgccc gcatagccca gcgccgggat gttactgcga
    15481 gctgtatcac tgatgatctc cattgccggc gcgcaggtgc gtgcgcccat cattgcgccg
    15541 aacaacagtg cgcggttcat tcgcaataca taagcaccga acaagaaaca gataaccacg
    15601 ggcacaagac tgacaatcaa tccggcaatc aacatctgac cgccaatcgc gcccagaccg
    15661 ttattaatac cgctaccggc gctcagacca acgcctgcca taaacaccat caagccgaac
    15721 tctttcacca tgcttaatgc cccttgcgga atgtaaccga aggtcgggtg gttagcacgc
    15781 ataaagccca gcataattcc ggcgaataac aacccggcag cgttccccat gccgaaactg
    15841 aatgtgctga actggaaggt gatcatcccg atcatcagcc caataacaaa gaaggcgcag
    15901 aatgccagca ggtcagtgac ctggctgtga atcgagataa agccgatgcg atcggcgatg
    15961 gtttttacgc ggcgggcatc gccgctgact tgtaaaacgt cacctttgtt aagcacgacg
    16021 ttgtcatcaa tcggcatctc aatctggcta cgaatgacgc ggttaaggaa gcaaccgtga
    16081 tcggtcaact tcagttgtgc gagacgttta cctacagcgt tatggttttt aacgaccact
    16141 tcttcagtga cgatacgcat gtcgagaagg tcacgatcga aaacttcttt accgttacgg
    16201 aagctgggat cgagtcgggc atgggcgtcg ggatagccta ccaacgctat ttcatcgccc
    16261 atttgtagca cggcatcacc gtctggattt gccagaatcc cgttacgtcg aatacgttca
    16321 atgtagcagc cggtttgtcg ataaataccc agttcacgca gatttttgcc gtcggtccag
    16381 gccaccagct ccgggccgac gcgataggcg cggatcaccg gtaaataaac cttacggttg
    16441 gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg cgctggtctg taagtcctga
    16501 tgctgcaatt tcggcaagta acgcgcacca acaatcaaac tcaccagacc gattaaatag
    16561 gttaaggcat acccgaggct cagattatcc agtgccagtg agagctgcct gctttccata
    16621 ccggaatgac gcagtgtatc gccagcaccg accagaaccg gtgtcgacgt catagagcct
    16681 gctaacatac cggccgtcag gccaatatcc cagccaaaca gcttacctaa ccctaaggcg
    16741 atcaccagcg cactgccaac catcaccagt gctaacatta ggtaattttt cccatcgcga
    16801 aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga cgcagaaaat aaacagcata
    16861 aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt gttggcctaa taacagcgat
    16921 acgactaaaa cgccaatgga attacccagt tggatcgaac caagtcgtaa ctttccgaga
    16981 catagcccaa gcgcgaggac cacaaataat aacagaatgt aattcccatt taacaattcg
    17041 gcgacgttta tattcacgga ggctaacttc ttgtttacta gtaagctgtt gaaagaaatg
    17101 gtaatttacg ataatgtttt ttaccagaat tcagggcgca gattcattca gcgcacctaa
    17161 acgatagtaa agtaacaata tattttacta gtgtaatcac attaggtatc aacggctata
    17221 tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg ctttatctta ctgaacgcca
    17281 cactggcgaa aaatgtgttc gatagacgca gtgtcaggag gaacgagtga aacataaaca
    17341 acgttgggcg ggggcaatct gctgttttgt cctcttcatt gtggtgtgcc tttttctggc
    17401 gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct gaaatcggct tgctgttttt
    17461 cattcttcct ggagcagttg ccagtttctt ttcacagcgt agagaagtcc tgaaacctct
    17521 atttggcgca atgctggcgg caccctgttc aatgcttatt atgcggctgt ttttttcacc
    17581 gacccgctca ttctggcaag agctggcatg gttactaagc gcggtgttct ggtgtgcgct
    17641 gggggcactg tgtttcttat ttatcagtag cttgtttaaa ccacagcaca gaaaaaatca
    17701 gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg tccagattct ctttcaccca
    17761 tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg acaaaaatct gcggcacggt
    17821 ttctacgggt ttacctgcct tttgttgtag atcttcttta gtgatccctt ccgcacgaat
    17881 atctacatac tgatactgaa aatcatcgcg ttcattgctc aatttctcag ccagatcttt
    17941 tgcacgcaca cagtaagggc aacccggacg accaaaaata acggtttgca ttatttctct
    18001 cctcatagat ttatgcctgt aatgatcacg ctaaaatgta ttcgctgaaa gtaggtttaa
    18061 cctgttgcat taattgctaa aagctataac tgttaaacac aatacagtga aaagttttag
    18121 actgaaggtt cactttgcag agggaagcgt atgcgcgcga tcggtaaatt gcctaaaagc
    18181 gtgttgatac tggaatttat cggaatgatg ttgctggcgg tggcgctgct gtcggtaagc
    18241 gactccctgt cgctgcctga gccattttct cggccagaag tgcagattct gatgattttt
    18301 ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta ttcttcaggt ggcaaaacgt
    18361 cttgccccac agctgatgaa ccgtccaccg cagtattcac gttcagaaag agaaaaagat
    18421 aatgacgcca accattgaac ttacttgtgg ccatcgctcc attcgccatt tcactgatga
    18481 acccatttcc gaggcgcagc gtgaggcgat tattaacagc gcccgtgcga cgtccagttc
    18541 cagttttttg cagtgcagta gcattattcg cattaccgac aaagcgttac gtgaagaact
    18601 ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg gcggagttct gggtgttctg
    18661 tgccgacttt aaccgccatt tacagatctg tccggatgct cagcttggcc tggcggaaca
    18721 attgctgctc ggtgtcgttg atacggcaat gatggcgcag aatgcattaa ccgcagcgga
    18781 atcgctggga ttgggcgggg tatatatcgg cggcctgcgc aataatattg aagcggtgac
    18841 gaaactgctg aaattaccgc agcatgttct gccgctgttt gggctgtgcc ttggctggcc
    18901 tgcggataat ccggatctta agccgcgttt accgtcctcc attttggtgc atgaaaacag
    18961 ctatcaaccg ctggataaag acgcactggc gcagtatgac gaacaactgg ctgaatatta
    19021 cctcacccgt ggcagcaata atcgccggga tacctggagc gatcatatcc gccgaacaat
    19081 cattaaagaa agccgcccat ttattctgga ttatttgcac aaacagggtt gggcgacgcg
    19141 ctaaaaccgc cacgccgatg tatgatacgc gggcttttga ccaggtctga cagagaggtg
    19201 cagggtgaaa attgccatat tgtcccggga tggaacgctc tattcgtgta agcggctgcg
    19261 tgaagccgct atacagcgcg gtcacctggt tgaaattctt gatccgcttt cttgctacat
    19321 gaacataaat cctgcggcgt cttctattca ctacaaaggc cgcaagttac cccattttga
    19381 cgcagtgatc ccgcgtattg gcaccgccat taccttttat gggacggcgg cactgcgcca
    19441 gttcgagatg ctggggagct atccgctcaa tgagtcggtc gccattgccc gggcgcgtga
    19501 caaattgcgt tccatgcaac tgctggcgcg tcagggcatc gacctgcctg tcacgggcat
    19561 tgcgcattcg ccggatgata ccagcgattt aatcgacatg gtcggtggtg cgccgctggt
    19621 ggtcaagttg gttgaaggca cgcagggaat tggcgtcgtg ctggcggaga cgcgtcaggc
    19681 ggcggaaagc gtgattgacg ctttccgcgg tctgaacgcg catattctgg tgcaggaata
    19741 tatcaaagag gcgcaagggt gcgatatccg ctgtctggtt gttggcgatg aagtggtcgc
    19801 tgcgattgaa cggcgggcga aagagggcga ttttcgttcc aatttgcatc gtggcggcgc
    19861 ggcaagcgtc gccagtatca caccacagga gcgtgaaatc gcgataaaag ccgcgcgaac
    19921 gatggcgctg gacgttgctg gtgtggatat tctgcgtgct aatcgcgggc cgttggtgat
    19981 ggaagtgaat gcgtcgccgg ggctggaagg aatagaaaaa accaccggta tcgacatcgc
    20041 gggtaaaatg atccgctgga tcgaacgcca cgctacgaca gaatattgcc tgaaaacggg
    20101 tggttagtcg caatcacatt actgatcatg gttttgcctg ctctttttgc gtaagctgtg
    20161 ccggtctttt tatcgaaaga ggttgtacaa aattatgaca tcgctggtcg ttcctggtct
    20221 ggatacgctg cgtcaatggc tcgatgacct ggggatgagt ttttttgaat gtgataactg
    20281 tcaggctctg catctgcccc atatgcagaa tttcgacggt gtctttgatg ccaaaatcga
    20341 tctgatcgat aacacgatcc tgttttctgc catggcggaa gtccgacctt cagccgtatt
    20401 gccgctggcg gcggatttat ctgccatcaa tgccagttcg ctgaccgtga aagcatttct
    20461 tgatatgcag gatgataatc tgccaaagct ggtggtttgc cagtctttat ccgttatgca
    20521 gggcgtaacc tatgagcagt ttgcatggtt cgtgcgtcag agcgaagagc agatttcgat
    20581 ggtcattctt gaagctaatg cccatcaact gctgttaccg actgacgatg aagggcaaaa
    20641 caacgttacc gaaaactatt tcctccactg ataactcctt tcgagcacgc agtcgctggt
    20701 acagtggctg cgcgctgcaa aattatctgc tgtttttaac cttttcttaa agattatttc
    20761 acttctcttg tgtcgatttg gctttatcac atagagcaaa tacgcataaa aatttgttaa
    20821 ataccgtttt ttaatccgag ctatagtctc aaaccctggc taaagttatt cttgcgatgc
    20881 ttttatatag cgagcagtgc tggccgggag agagttctct tttcttacac cgcgccgata
    20941 aaaaatatgc acgtttattg catatctttc agtgtgacaa cttttgttcg tttgttaacg
    21001 aactttcaga aggaaagaga tatgaccgcc ttaaataaaa aatggctttc gggtctggtt
    21061 gcgggtgctc tgatggccgt ctctgtcggc acgctcgcgg ctgaacaaaa aacactccac
    21121 atttataact ggtctgatta tatcgccccc gacacggtgg ctaactttga aaaagaaacc
    21181 ggtattaaag tcgtctacga tgttttcgac tctaacgaag tactggaagg caaattaatg
    21241 gccgggagca ccggctttga cctggtggtg ccgtcagcaa gctttcttga acgccagctg
    21301 acagcgggag ttttccagcc gctggataaa agcaaattgc cggagtggaa gaatctcgac
    21361 ccggaactgc tgaagttggt cgccaaacac gatcccgaca ataaatttgc tatgccctat
    21421 atgtgggcga cgaccgggat tggctataac gtcgataaag tcaaagcggt gctgggcgaa
    21481 aacgcgcccg tcgatagctg ggacttgatc ctcaagcctg aaaatctgga aaaactgaaa
    21541 agctgcggtg tatctttcct cgatgcgcca gaagaagttt ttgctaccgt gctgaattat
    21601 ctcggcaaag atcccaacag cactaaagcg gatgattaca ccggaccggc taccgatctg
    21661 ctgttaaagc tgcgcccgaa cattcgttat ttccattcat ctcaatatat taacgacctg
    21721 gcaaacggcg atatttgcgt cgctatcggc tgggcaggtg atgtctggca ggcgtcaaac
    21781 cgcgcgaagg aagcgaagaa tggcgtgaat gtctcgttct cgattccaaa agaaggggcg
    21841 atggcgttct ttgatgtatt cgccatgcct gcggatgcca aaaataaaga cgaagcctat
    21901 cagttcctga attacctgct gcgtcctgat gtggtggcac atatctctga ccatgtgttc
    21961 tatgctaacg ccaataaagc agccacgccg ctggtgagtg cggaagtccg tgataacccg
    22021 ggtatttatc cgcctgcgga tgtccgcgcg aagttgttca ctctgaaagt acaggctccg
    22081 aaaatcgacc gtgtgcgcac ccgcgcgtgg accaaagtga agagcggaaa ataatccgca
    22141 gccttagatg ccgggcggga gcgccactcc ctccggcaat tcgcaccaac atggtgcgct
    22201 tgcacacatt taatgccgga gagcagccgt gaatgacgct atccctcgcc cccaggcgaa
    22261 aacccgtaag gcgctgaccc cgctattaga aatccgcaat ctgaccaaat cctacgatgg
    22321 tcagcatgcg gttgatgatg tcagcctgac catctacaaa ggtgaaatct ttgcgctgct
    22381 gggcgcatcc ggctgtggca agtccacgct gctgcgtatg ctggcaggtt tcgaacaacc
    22441 ttcagccggg cagattatgc ttgatggcgt cgatttgtca caggtgccgc cttacctgcg
    22501 ccccatcaat atgatgtttc agtcttacgc gctgtttccg catatgaccg tggaacagaa
    22561 catcgctttt ggcctgaaac aggacaaact accgaaagcg gaaattgcca gccgggtcaa
    22621 tgagatgctc ggccttgtgc atatgcagga gttcgccaaa cgcaaaccgc atcagctttc
    22681 cggtggtcag cgacagcgtg tggcactggc ccgaagcctt gccaagcgcc cgaaactatt
    22741 actgctcgac gagccgatgg gcgcgctgga taaaaagctg cgtgaccgga tgcagcttga
    22801 agtggtggat attctggaac gcgtcggtgt gacttgtgtg atggtcaccc acgatcagga
    22861 agaggcgatg accatggcgg ggcgtatcgc cattatgaat cgtgggaaat ttgtccagat
    22921 tggcgaaccg gaagagatct acgagcatcc gactacccgc tatagcgccg aatttattgg
    22981 ctcggttaat gtctttgaag gtgtactcaa agagcgtcag gaagatggtc tggtgcttta
    23041 ttcgccgggg ctggtgcatc cactgaaagt cgatgcggat gcttcagtgg tcgataacgt
    23101 gccggtacac gtagcgctgc gtccggaaaa aatcatgctt tgcgaagagc cgcccgccaa
    23161 tggttgtaac ttcgcggtgg gggaggtgat acacattgcc tatctcggcg atctttcggt
    23221 gtatcacgtt cgtctgaaaa gtgggcagat gattagcgcc cagctacaaa acgcccatcg
    23281 tcatcgtaaa gggttaccga cctggggcga cgaagtgcgt ttgtgctggg aagtggacag
    23341 ctgtgtggtg ctgacggttt aaggagcaaa gatgagtaca cttgaacctg ctgcccagtc
    23401 gaaaccgccg ggcggattta agctgtggtt gtcgcagttg caaatgaagc atgggcgcaa
    23461 actggtcatt gcgttgccgt atatctggtt gatcttgctg tttctgctgc catttctgat
    23521 tgtctttaaa ataagcctgg cggagatggc gcgcgctatt ccaccttata ccgaactgat
    23581 ggagtgggct gacgggcaac tgtccatcac ccttaatttc ggtaatttcc tgcaactgac
    23641 cggcgatccg ctctatttcg atgcttatct ccagtcgtta caggtggcgg cgatttcgac
    23701 attttgctgt ttactgatcg gctatccgct ggcgtgggcg gtggcgcaca gtaagccttc
    23761 gacccgtaat atcttattac tactggtgat cctgccgtcg tggacctcgt ttctgattcg
    23821 tgtctacgcc tggatgggaa tattaaaaaa caacggtgtg ctgaataatt ttctgctgtg
    23881 gctgggggtt atcgatcaac cgctgaccat tcttcatacc aatctggcag tttatattgg
    23941 cattgtttac gcctacgtgc cgtttatggt gctgccgatt tataccgcgt tgattcgtat
    24001 tgattattcg ctggtggaag cagcgctgga tctcggtgca cgaccgctga aaacgttctt
    24061 tactgtgatc gtgccgctga ctaaaggtgg gattattgcc ggatcgatgc tggtgtttat
    24121 cccggctgtg ggcgagtttg tgatcccgga actgctcggt ggcccggaca gcatcatgat
    24181 cgggcgcgtg ctatggcaag agtttttcaa taaccgcgac tggccggtgg cctcggcggt
    24241 agcgatcatc atgttgctgc tgctaattgt gccgataatg tggtttcaca aacaccagca
    24301 aaaaagcgtg ggagaacacg gatgaataat ttaccggtag ttcgttcgcc ctggcggatt
    24361 gtgattttgc tgttgggctt cacctttctc tacgcgccaa tgctgatgct ggttatctat
    24421 tcgtttaaca gctcgaagct ggtgacggtg tgggccggct ggtcaacgcg ctggtatggc
    24481 gaattattgc gcgatgacgc gatgatgagt gcggttggtt taagtctgac aattgcggcc
    24541 tgtgcggcga cggcggcggc gatcctcgga actattgcgg cggtggtgct ggtgcgcttt
    24601 ggcaggtttc gcggatcaaa tggctttgcc tttatgatca ccgcgccgct ggtaatgcca
    24661 gatgtcatca cgggcttgtc gctgttgtta ttattcgtcg cgcttgctca tgccattggc
    24721 tggcctgcgg accgcggtat gcttaccatc tggctggcgc atgtcacttt ctgtacggct
    24781 tatgtggcag tcgttatttc gtcgcgtctt cgggaactgg atcgctcgat agaagaagca
    24841 gcgatggatc tcggtgcgac gccgctgaaa gtgtttttcg tcattacgct accgatgatc
    24901 atgcccgcgg tcatttctgg ctggttactg gcttttactt tgtcgcttga tgatctggtg
    24961 atcgccagct ttgtatccgg gccgggagcc accacgttac cgatgctggt cttttccagc
    25021 gtgcggatgg gggtgaatcc ggaaatcaac gccctagcaa cattgatcct cggcgcggtc
    25081 ggaattgtcg gatttatcgc ttggtatctg atggctcgcg cagaaaaaca gcggatacgc
    25141 gacatccagt gtgcaagacg tggctgaaga cactaaaatt tgccaacctg gctacataat
    25201 gccgcgtatg tcgcggcatt gttttcatgg aagacgaaac gttgggattt tttaagaaaa
    25261 catcttcatc tcatgctcgc ctgaatgtgc ctgcgcttgt gcaggtggcg gcgctcgcca
    25321 ttattatgat ccgtggcctc gacgtgctga tgattttcaa tacgctgggc gtgcgcggta
    25381 ttggcgagtt cattcatcgc agcgtacaaa cctggagttt aacgctggtc tttttaagca
    25441 gtctggtgct ggttttcatt gagatctggt gtgcgttttc actggtgaaa gggcgtcgct
    25501 gggcgcgctg gctgtatctg ctgacacaaa tcactgccgc aagttacttg tgggcggctt
    25561 cgctggggta cggttatccg gagctgttca gcattcccgg tgaatcaaaa cgtgaaatct
    25621 tccatagcct gatgctgcag aagctgccgg atatgctcat cctgatgctg ctgttcgttc
    25681 cctcgaccag tcggcggttc ttccagttgc aataatgtgt ataatcgtcg cccctgatga
    25741 tgtgaaggtc aatgtatgca gtgcgcactt tacgacgcgg gtcgctgtcg ttcctgtcag
    25801 tggataacgc agccgattcc agagcaactc tccgctaaaa ccgccgatct taaaaatctg
    25861 ctcgccgact ttccggttga ggaatggtgc gcgccggtgt caggcccgga acaagggttt
    25921 cgtaataaag ccaaaatggt ggtgagtggt agcgttgaaa aaccactgct cggtatgctg
    25981 catcgcgacg gcacaccgga agacctttgt gactgcccgc tttatcctgc ctcatttgcg
    26041 cccgtttttg cggcgctaaa accgtttatc gcccgagcgg ggttaacgcc ctacaacgtg
    26101 gcgcgtaagc gtggcgaact gaaatacatt ctgctgactg aaagccagag cgatggcggc
    26161 atgatgctgc gctttgtact gcgttctgaa accaaactag cacaactgcg taaggcattg
    26221 ccgtggttac aggcacaatt gccgcagcta aaagttatca ccgtcaatat tcagccggta
    26281 catatggcga ttatggaagg ggagacggag atctacctga ccgaacaaca ggcgctggta
    26341 gagcgtttta acgatgtgcc gctgtggatc cgtccgcaaa gtttcttcca gactaatccg
    26401 gcggtcgcca gccagctgta cgctaccgcg cgcgactggg tacgacagct gccggttaaa
    26461 catatgtggg atctgttctg cggtgtgggg ggctttggtt tacactgcgc gacgcctgac
    26521 atgcagttaa ccgggatcga aattgcgcta gaggccattg cctgtgcaaa gcagtcagcc
    26581 gctgaactgg gcttaacgcg tctgcaattt caggcgctgg actccactca gtttgccacc
    26641 gctcaggggg aggtgccgga gctggtgctg gttaacccgc cgcgccgcgg cattggtaaa
    26701 ccgctgtgtg attatctctc aacgatggca ccgcgtttta ttatctactc cagctgtaac
    26761 gcccaaacca tggcgaaaga tatccgcgaa ctgccaggtt accgcatcga acgggtacag
    26821 ctttttgata tgttcccgca caccgcgcac tatgaagtgc tgacgctgct ggtaaaaatg
    26881 taaatacatc attcaaatca atatcacctc ggcatacatc catccaaaat aataatcgtg
    26941 atcgatcgat taataataac cgatcgatca cggttatata ttgctctatt tttgcaaatc
    27001 aaatcacaaa taatcaaata agatctttaa ttgaactgtt ttgattacta cagtgcactt
    27061 accgaataca tacagaggca ttgcttagcg aaacacaaaa tttcctgaga cattattaac
    27121 gtcagcagaa ggttcatgca ttgacaacgg aaatgtacgc gtatggaggg cgttccgacg
    27181 atgtttgcca aattcataga tgttattcaa acgtttttaa ctgaaccggc aattttaatt
    27241 ggtttgctgg tgggtattgg ttatgccctt gataagaaat cgccaataaa aattattacc
    27301 ggcatggtca gcgccatggt gggattaatg atggtgttat ttggtggttt ccagttttcc
    27361 gcaacattta aaccggtagc agaagccgta agtaaagcct atggtgttca cggatattta
    27421 atggattcct acgcaatgaa agcggcaacg caaattgcat tgggggataa ttttggttat
    27481 gtcgggtatg tatttgtact ggcgtttttt accaacctgc tgctggtatt atttggtcgg
    27541 tatactggtg ccaaagggat atttttgaca gggaataccg gtgtttcaca ttctcaggcg
    27601 gtattatggt tgattgtctt ttggctgggt tttagctgga ctacatccat tattattgcc
    27661 ggaatattaa cgggtgtatt ctgggcgttc tccaccacgc tcattgttaa gcccattgcc
    27721 aaagttacca aagatgccgg ttttaccatt gctcataacc agatgttggg attgtggttt
    27781 ttctccaaat tcgcccataa atttggtgac cctgaaaagc acgatgctga aaacctgaaa
    27841 cttcccgggt ggctggcgat ttttaaccat aacgtaacgg ctatcgccat tgtgatgacg
    27901 ctgtttgtcg gtggtttctt gctatcaacg ggtatcgata atgtccagtt aatggcgaaa
    27961 ggcaagccct ggtacatcta tatcatcaac cttggcttac agttctccat gtatatggtc
    28021 attctgctgc agggtgtgcg catgatggtg ggcgaaatta acggttcgtt taaaggctgg
    28081 caagatcgct ttattcctaa cgctattcct gctgtcgatg ttgcagcgct gttacctttt
    28141 tcacccaatg ccgcaacgtt aggcttcgtc ttctgtactt ttggcaccat tttttcgatg
    28201 gggatcttat tactggttca cagcccaatt atggtattgc ctggctttgt acctctgttt
    28261 ttctctggcg gtccaattgg cgtattagca aaccgcatgg gcggataccg ttccgtaatt
    28321 atctgtacgt tcttgctggg tattattcag accttcggta cggtgtgggc tattccgtta
    28381 accgggcttg ctgaaaatgg cgtcggctgg acaggaatat ttgactgggc aaccgtatgg
    28441 cctgctattt gtgaagttct gaaatttatc gctgcaacat tccatcttgg tccttacgcg
    28501 ggttaattaa atattaaggg tttaaaaatg aaagatacaa ttaatattct gtttgtctgt
    28561 ggttacggtg taggcagtag cgttatgtta caaacggtcg ttaaaaaagc gctgaccaaa
    28621 tatgatttca aagttgaaat ggaacatacc gccgcaggtg aagtcggcgg atttaccgac
    28681 tgggcggata tttttgctat ttccaaaaaa ttactcgatg tggttagtct tgatccgaaa
    28741 caagggcaat atttgattcc gatagagaat attatggacg gtgaaactat tggtcagcaa
    28801 atttatgaag tggttcagca acattttccg catttaatta atcaataagg tagggcgtaa
    28861 tgagagcgat tattctgctg ttcgatagtc tgaataaacg ttatctgcca ccttatggcg
    28921 atgcgttaac caaagcgcct aatttccaac gtctggcggc tcatgccgcc acctttgaaa
    28981 acagttacgt cggcagtatg ccgtgtatgc ctgcccggcg ggagttgcat accgggcgat
    29041 gtaacttcct gcatcgggag tgggggccgt tagaaccgtt tgacgattcc atgccggagc
    29101 tgcttaaaaa ggcggggatc tatacccacc tgataagcga tcatttgcat tactgggaag
    29161 atggcggcgg gaattatcat aatcgctaca gttcgtggga gattgtgcgc ggacaggaag
    29221 gcgatcactg gcatgcaagc gttgcgcaac cgcctattcc cgaggtgcta cgggtgccgc
    29281 aaaagcagac cggtggcggt gtttctggtc tgtggcgcca tgactgggca aaccgggaat
    29341 atattcagca ggaagcggat tttccacaaa ctaaagtgtt tgatgcaggc tgtgccttta
    29401 tccacaaaaa tcacgcggaa gataactggt tattgcagat cgaaacgttt gatccgcacg
    29461 agccgtttca cacaacggaa gagtatcttt ccttgtatga agataactgg gatggaccgc
    29521 attatgactg gccgcgtggc cgggtgcagg aaagcgacga ggccgtggag cacatccgtt
    29581 gccggtatcg ttcgctggtg tcgatgtgcg atcgcaacct ggggcgcatt ctcgacctga
    29641 tggatgagca cgatctgtgg cgagatacca tgctgattgt cggcactgac cacggctttt
    29701 tactcggtga gcatggctgg tgggcgaaaa accagatgcc ttattacaac gaagttgcca
    29761 ataatccgtt gtttatctgg aacccgcgca gtggtgtaaa aggagagcgg cgacaggcac
    29821 tggtacaaat gatcgactgg gcgccaacgt tgtatgactt ttttcaacag ccagtgccgc
    29881 ccgatgtgca ggggcaaccg ctggcgaaaa cggtcagtca cgatgaacca gtacgcagct
    29941 cggcgatgtt tggtgttttc agtggtcatg ctaacgtaac tgatgggcgt tatgtgtata
    30001 tgcgtgcagc gctgccgggg cgtgaggatg atattgccaa ctacacgttg atgtcctgca
    30061 aaatgaacag ccgctatccg gtggatgaga tgcgggcttt atcgctggcc ccaccgtttc
    30121 gttttaccaa agggttacag gtattacgca tcccggcaca ggaaaaatat aaggggttga
    30181 atcagtttgg tcatttgctg tttgatctgc aaaacgatcc gcagcagcta catccgattc
    30241 atgatgatgt gatcgagtcc cggatgatcg cgttgctgat tcagttgatg aaagataatg
    30301 atgcgccgcc agagcagttt cagcgcctgg gattagcgta gagaagtgat tttgccggag
    30361 tgggtgttcg cccactccgg caaatgaata attattgcgg gaaccactga tcgctgattt
    30421 gttgatatgt accgtcagct ttaattgctg ccagcgcgtt attcagtttt tccagcagag
    30481 ctttgttatc cggacgtaca gcgataccca gaccggtgcc aaagtattgc ggatcggtca
    30541 ctttttcagt agcaacgccc agttgcggat tggttttcag ccattcatta accaccgcag
    30601 tgtcaccaaa tacgccatca atacggctat ttttcagatc gataaaggca ttttgataac
    30661 tgtcatagga gaccgttttc acttccgggt gctgatcctg aatatatttc tggtgcgtag
    30721 taccgttttc catcccaatg cgtttgcctt tcaggtcggc aaacgttttg taggtatctt
    30781 ttttggcaat cacgacggct gagttttcgt aatagggcgt ggtgaacgat acctgcttgc
    30841 tacgatctgg tgtgatatcc ataccggaga ttacggcgtc atatttcctg aatttcagtg
    30901 acgggatcag gctgtcgaac gcgtgattgg taaaagtaca ttctgcctgc atctgtttgc
    30961 ataacgcttt tgctaggtcg atatcaaagc cgacgatctg gttattggca tccatcgact
    31021 cgaacggcgg ataggtcgct gatacgccaa aattgatttt ctctgcggca gaagcgccga
    31081 acgcacagga tgccagtaat gcggcaagaa ctaacttttt catgatggaa ctcccgtctg
    31141 tcaatcttat gattattggc cgtgtctgcg gcatgggata acaatgccat ca
//
LOCUS       DAZFBR010000052        30596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFBR010000052 DAZFBR010000000
VERSION     DAZFBR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.052"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            245..628
                     /gene="secE"
                     /locus_tag="ACHJZ2_002550"
     CDS             245..628
                     /gene="secE"
                     /locus_tag="ACHJZ2_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO4568636.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            630..1175
                     /gene="nusG"
                     /locus_tag="ACHJZ2_002551"
     CDS             630..1175
                     /gene="nusG"
                     /locus_tag="ACHJZ2_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO4568637.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1334..1762
                     /gene="rplK"
                     /locus_tag="ACHJZ2_002552"
     CDS             1334..1762
                     /gene="rplK"
                     /locus_tag="ACHJZ2_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO4568638.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1766..2470
                     /gene="rplA"
                     /locus_tag="ACHJZ2_002553"
     CDS             1766..2470
                     /gene="rplA"
                     /locus_tag="ACHJZ2_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO4568639.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2762..3259
                     /gene="rplJ"
                     /locus_tag="ACHJZ2_002554"
     CDS             2762..3259
                     /gene="rplJ"
                     /locus_tag="ACHJZ2_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO4568640.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3326..3691
                     /gene="rplL"
                     /locus_tag="ACHJZ2_002555"
     CDS             3326..3691
                     /gene="rplL"
                     /locus_tag="ACHJZ2_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO4568641.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4011..8039
                     /gene="rpoB"
                     /locus_tag="ACHJZ2_002556"
     CDS             4011..8039
                     /gene="rpoB"
                     /locus_tag="ACHJZ2_002556"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO4568642.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8116..12339
                     /gene="rpoC"
                     /locus_tag="ACHJZ2_002557"
     CDS             8116..12339
                     /gene="rpoC"
                     /locus_tag="ACHJZ2_002557"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO4568643.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12552..12995
                     /locus_tag="ACHJZ2_002558"
     CDS             12552..12995
                     /locus_tag="ACHJZ2_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFO4568644.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(13430..14563)
                     /gene="thiH"
                     /locus_tag="ACHJZ2_002559"
     CDS             complement(13430..14563)
                     /gene="thiH"
                     /locus_tag="ACHJZ2_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO4568645.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14560..15330)
                     /gene="thiG"
                     /locus_tag="ACHJZ2_002560"
     CDS             complement(14560..15330)
                     /gene="thiG"
                     /locus_tag="ACHJZ2_002560"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO4568646.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15332..15532)
                     /gene="thiS"
                     /locus_tag="ACHJZ2_002561"
     CDS             complement(15332..15532)
                     /gene="thiS"
                     /locus_tag="ACHJZ2_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO4568647.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15516..16271)
                     /gene="thiF"
                     /locus_tag="ACHJZ2_002562"
     CDS             complement(15516..16271)
                     /gene="thiF"
                     /locus_tag="ACHJZ2_002562"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO4568648.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16264..16899)
                     /gene="thiE"
                     /locus_tag="ACHJZ2_002563"
     CDS             complement(16264..16899)
                     /gene="thiE"
                     /locus_tag="ACHJZ2_002563"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO4568649.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16899..18794)
                     /gene="thiC"
                     /locus_tag="ACHJZ2_002564"
     CDS             complement(16899..18794)
                     /gene="thiC"
                     /locus_tag="ACHJZ2_002564"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO4568650.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18875..18975)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19027..19503)
                     /gene="rsd"
                     /locus_tag="ACHJZ2_002565"
     CDS             complement(19027..19503)
                     /gene="rsd"
                     /locus_tag="ACHJZ2_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO4568651.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19598..20371
                     /gene="nudC"
                     /locus_tag="ACHJZ2_002566"
     CDS             19598..20371
                     /gene="nudC"
                     /locus_tag="ACHJZ2_002566"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO4568652.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20411..21475
                     /gene="hemE"
                     /locus_tag="ACHJZ2_002567"
     CDS             20411..21475
                     /gene="hemE"
                     /locus_tag="ACHJZ2_002567"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO4568653.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21485..22156
                     /gene="nfi"
                     /locus_tag="ACHJZ2_002568"
     CDS             21485..22156
                     /gene="nfi"
                     /locus_tag="ACHJZ2_002568"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO4568654.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22199..22789
                     /gene="yjaG"
                     /locus_tag="ACHJZ2_002569"
     CDS             22199..22789
                     /gene="yjaG"
                     /locus_tag="ACHJZ2_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO4568655.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            22976..23248
                     /gene="hupA"
                     /locus_tag="ACHJZ2_002570"
     CDS             22976..23248
                     /gene="hupA"
                     /locus_tag="ACHJZ2_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO4568656.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23261..23956
                     /gene="yjaH"
                     /locus_tag="ACHJZ2_002571"
     CDS             23261..23956
                     /gene="yjaH"
                     /locus_tag="ACHJZ2_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO4568657.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23958..24383)
                     /gene="zraP"
                     /locus_tag="ACHJZ2_002572"
     CDS             complement(23958..24383)
                     /gene="zraP"
                     /locus_tag="ACHJZ2_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO4568658.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24621..25997
                     /gene="zraS"
                     /locus_tag="ACHJZ2_002573"
     CDS             24621..25997
                     /gene="zraS"
                     /locus_tag="ACHJZ2_002573"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO4568659.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            25994..27319
                     /gene="zraR"
                     /locus_tag="ACHJZ2_002574"
     CDS             25994..27319
                     /gene="zraR"
                     /locus_tag="ACHJZ2_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO4568660.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27316..28605)
                     /gene="purD"
                     /locus_tag="ACHJZ2_002575"
     CDS             complement(27316..28605)
                     /gene="purD"
                     /locus_tag="ACHJZ2_002575"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO4568661.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28617..30206)
                     /gene="purH"
                     /locus_tag="ACHJZ2_002576"
     CDS             complement(28617..30206)
                     /gene="purH"
                     /locus_tag="ACHJZ2_002576"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO4568662.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7184 a         7928 c         8370 g         7114 t
ORIGIN      
        1 aaagttctga gctaattgcc gataacattt gacgcaatgc gcactaaaag ggcatcattt
       61 gatgcccttt ttgcacgctt tcgtaccaga acctggctca tcagtgattt tctttgtcat
      121 aatcattgct gagacaggct ctgttgaggg cgtataatcc gaaaagctaa tacgcgtttc
      181 gatttggttt gcctcgcgat cgcggggtga aaatgtttgt agaaaacttc tgacaggttg
      241 gtttatgagt gcgaataccg aagctcaagg aagcgggcgc ggcctggaag cgatgaagtg
      301 ggtcgttgtg gtggcattgc tcttggtggc gattgtcggc aactatcttt atcgcgacat
      361 tatgctgccg ctgcgtgcgc tggccgtagt aattctgatt gctgcagcgg gtggtgtcgc
      421 gctgttaacg acaaaaggta aagctaccgt tgcttttgcc cgtgaagcgc gtaccgaagt
      481 ccgtaaggtc atttggccga ctcgccagga aacattgcac accacgctga ttgtggctgc
      541 ggttaccgca gtaatgtcac tgatcctgtg gggactggat ggtattctgg ttcgcctggt
      601 atcctttatc actggcctga ggttctgaga tgtctgaagc tcctaaaaag cgctggtacg
      661 tcgttcaggc gttttccggt tttgaaggcc gcgtagcaac gtcgctgcgt gagcatatca
      721 aattacacaa catggaagat ttgtttggtg aagtcatggt accaaccgaa gaagtggttg
      781 aaatccgtgg cggtcagcgt cgcaaaagcg aacgtaaatt cttccctggc tacgtcctcg
      841 ttcagatggt gatgaacgac gcgagctggc acctggtgcg cagcgtaccg cgtgtgatgg
      901 gcttcatcgg cggtacttcc gatcgtcctg cgccaatcag cgataaagaa gtcgatgcga
      961 ttatgaaccg cctgcagcag gttggtgata agccgcgtcc gaaaacgctg tttgaaccgg
     1021 gtgaaatggt ccgtgttaat gatggtccgt tcgctgactt caacggtgtt gttgaagaag
     1081 tggattacga gaaatctcgt ctgaaagtgt ctgtttctat cttcggtcgt gcgaccccgg
     1141 tagagctgga cttcagccag gttgaaaaag cctaacccag cgatcaaaaa agcggcgatt
     1201 taatcgttgc acaaggcgcg agattggaat acaatttcgc gccttttgtt tttatgggtc
     1261 ttgcccgtaa aacgattttt tatatcacgg ggagcctctc agaggcgtta ttacccaact
     1321 tgaggaattt ataatggcta agaaagtaca agcctatgtc aagctgcagg ttgcagctgg
     1381 tatggctaac ccgagtccgc cagtaggtcc ggctctgggt cagcagggcg taaacatcat
     1441 ggaattctgc aaagcgttca acgcaaaaac tgattccatc gaaaaaggtc tgccgattcc
     1501 ggtagtaatc accgtttacg ctgaccgttc tttcactttc gttaccaaga ctccgccggc
     1561 agcagttctg ctgaaaaaag cggctggtat caagtctggt tccggtaagc cgaacaaaga
     1621 caaagtgggt aaaatttccc gcgctcagct gcaggaaatc gcgcagacca aagctgccga
     1681 catgactggt gccgacattg aagcgatgac tcgctccatc gaaggtactg cacgttccat
     1741 gggcctggta gtggaggact aagaaatggc taaactgacc aagcgcatgc gtgttatccg
     1801 cgagaaagtt gatgcaacca aacagtacga catcaacgaa gctatcgctc tgctgaaaga
     1861 gctggcgact gctaaattcg tagaaagcgt ggacgtagct gttaacctcg gcatcgacgc
     1921 tcgtaaatct gaccagaacg tacgtggtgc aactgtactg ccgcacggta ctggccgttc
     1981 cgttcgcgta gccgtattta cccagggtgc aaacgctgaa gctgctaaag ctgcaggtgc
     2041 tgaactggta ggtatggaag atctggctga ccagatcaag aaaggcgaaa tgaactttga
     2101 cgttgttatt gcttctccgg atgcaatgcg cgttgttggc cagctgggcc aggttctggg
     2161 tccgcgcggc ctgatgccaa acccgaaagt gggtactgta acgccgaacg ttgctgaagc
     2221 ggttaaaaac gctaaagctg gccaggttcg ttaccgtaac gacaaaaacg gcatcatcca
     2281 caccaccatc ggtaaagtgg actttgacgc tgacaaactg aaagaaaacc tggaagctct
     2341 gctggttgcg ctgaaaaaag caaaaccgac tcaggcgaaa ggcgtgtaca tcaagaaagt
     2401 tagcatctcc accaccatgg gtgcaggtgt tgcagttgac caggctggcc tgagcgcttc
     2461 tgtaaactaa tgcctttacg tgggcggtga ttttgtctac aatcttaccc ccacgtatgc
     2521 taacgaaagt tagcagctaa aaagatttgt tcgttggagc ctggcctatc caggcctccg
     2581 tcgaagaccg caggagtttc gcaagaaact taatcccctg cgtagacggt gacagaacgc
     2641 taagattatt cttttatatt ctggcttgtt tctgctcacc gtaattaaga cgctctctcc
     2701 gtttggagga gtgaagtgag ttccagagat tttctctggc aaacatccag gagcaaagct
     2761 aatggcttta aatcttcaag acaaacaagc gattgttgct gaagtcagcg aagtagccaa
     2821 aggcgcgctg tctgcagtag ttgcggattc ccgtggcgta actgtagata aaatgactga
     2881 actgcgtaaa gcaggtcgcg aagctggcgt atacatgcgt gttgttcgta acaccctgct
     2941 gcgccgtgct gttgaaggta ctccgttcga gtgcctgaaa gacgcgtttg ttggtccgac
     3001 cctgattgca tactctatgg aacacccggg cgctgctgct cgtctgttca aagagttcgc
     3061 gaaagcgaat gcaaaatttg aggtcaaagc cgctgccttt gaaggtgagc tgatcccggc
     3121 gtctcagatc gaccgcctgg caactctgcc gacctacgaa gaagcaattg cacgcctgat
     3181 ggcaaccatg aaagaagctt cggctggcaa actggttcgt actctggctg ctgtacgcga
     3241 tgcgaaagaa gctgcttaat cgcagttatc tttttaacgc attcgcttac gtataaactt
     3301 attctgatat tcaggaacaa tttaaatgtc tatcactaaa gatcaaatca ttgaagcagt
     3361 tgcagctatg tctgtaatgg acgttgtaga actgatctct gcaatggaag aaaaattcgg
     3421 tgtttccgct gctgctgctg tagctgtagc tgctggcccg gttgaagctg ctgaagaaaa
     3481 aactgaattc gacgtaattc tgaaagctgc tggcgctaac aaagttgctg ttatcaaagc
     3541 agtacgtggc gcaactggcc tgggtctgaa agaagctaaa gacctggtag aatctgcacc
     3601 ggctgctctg aaagaaggcg tgagcaaaga cgacgcagaa gcactgaaaa aagctctgga
     3661 agaagctggc gctgaagttg aagttaaata agccaaccct tccggttgca gcctgagaaa
     3721 tcaggctgat ggctggtgac tttttagtca ccagcctttt tgcgctgtaa ggcaccagta
     3781 gcgtttcaca ctgtttgact actgctgtgc ctttcaatgc ttgtttctat cgacgactta
     3841 atatactgcg acaggacgtc cgttctgtgt aaatcgcaat gaaatggttt aagcgtgata
     3901 gcaacaggca ttgcggaaag tgttccattt tccggtcaac aaaatagtgt tgcacaaact
     3961 gtccgctcaa tggacagatg ggtcgacttg tcagcgagct gaggaaccct atggtttact
     4021 cctataccga gaaaaaacgt attcgtaagg attttggtaa acgtccacaa gttctggatg
     4081 taccttatct cctttctatc cagcttgact cgtttcagaa atttatcgag caagatcctg
     4141 aagggcagta tggtctggaa gctgctttcc gttccgtatt cccgattcag agctacagcg
     4201 gtaattccga gctgcaatac gtcagctacc gccttggcga accggtgttt gacgtccagg
     4261 aatgtcaaat ccgtggcgtg acctattccg caccgctgcg cgttaaactg cgtctggtga
     4321 tctatgagcg cgaagcgccg gaaggcaccg taaaagacat taaagaacaa gaagtctaca
     4381 tgggcgaaat tccgctcatg acagacaacg gtacctttgt tatcaacggt actgagcgtg
     4441 ttatcgtttc ccagctgcac cgtagtccgg gcgtcttctt tgactccgac aaaggtaaaa
     4501 cccactcttc gggtaaagtg ctgtataacg cgcgcatcat cccttaccgt ggttcctggc
     4561 tggacttcga attcgatccg aaggacaacc tgttcgtacg tatcgaccgt cgccgtaaac
     4621 tgcctgcgac catcattctg cgtgccctga actacaccac agagcagatc ctcgacctgt
     4681 tctttgaaaa agttatcttt gaaatccgtg ataacaagct gcagatggaa ctggtgccgg
     4741 aacgcctgcg tggtgaaacc gcatcctttg acatcgaagc taacggtaaa gtgtacgtag
     4801 aaaaaggccg ccgtatcact gcgcgccaca ttcgccagct ggaaaaagac gacgtcaaac
     4861 tgatcgaagt cccggttgag tacatcgcag gtaaagtggt tgctaaagac tatattgatg
     4921 agtctaccgg cgagctgatc tgcgcagcga acatggagct gagcctggat ctgctggcta
     4981 agctgagcca gtctggtcac aagcgtatcg aaacgctgtt caccaatgat ctggatcacg
     5041 gcccgtatat ctctgaaacc ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg
     5101 tagaaatcta ccgcatgatg cgccctggcg agccgccgac tcgtgaagca gcggaaagcc
     5161 tgttcgagaa cctgttcttc tccgaagacc gttatgacct gtctgcggtt ggtcgtatga
     5221 agttcaaccg ttctctgctg cgcgaagaaa tcgaaggttc tggtatcctg agcaaagacg
     5281 acatcattga tgttatgaaa aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg
     5341 atatcgacca cctcggcaac cgtcgtatcc gttccgttgg cgaaatggcg gaaaaccagt
     5401 tccgcgttgg cctggtacgt gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc
     5461 tggataccct gatgcctcag gatatgatca acgccaagcc gatttccgca gcagtgaaag
     5521 agttcttcgg ttccagccag ctgtctcagt ttatggacca gaacaacccg ctgtctgaga
     5581 ttacgcacaa acgtcgtatc tccgcactcg gcccaggcgg tctgacccgt gaacgtgcag
     5641 gcttcgaagt tcgagacgta cacccgactc actacggtcg cgtatgtcca atcgaaaccc
     5701 ctgaaggtcc gaacatcggt ctgatcaact ctctgtccgt gtacgcacag actaacgaat
     5761 acggcttcct tgagactccg tatcgtaaag tgactgacgg tgttgtaact gacgaaattc
     5821 actacctgtc tgctatcgaa gaaggcaact acgttatcgc ccaggcgaac tccaacctgg
     5881 atgaagaagg ccacttcgta gaagacctgg taacctgccg tagcaaaggc gaatccagct
     5941 tgttcagccg tgaccaggtt gactacatgg acgtatccac ccagcaggtg gtatccgtcg
     6001 gtgcgtccct gatcccgttc ctggaacacg atgacgccaa ccgtgcattg atgggtgcga
     6061 acatgcaacg tcaggccgtt ccgactctgc gtgctgataa gccgctggtt ggtactggta
     6121 tggaacgtgc tgttgccgtt gactccggtg taactgcggt tgctaaacgt ggtggtgtcg
     6181 ttcagtacgt ggatgcttcc cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg
     6241 gtgaagcagg tatcgacatc tacaacctga ccaaatacac ccgttctaac cagaacacct
     6301 gtattaacca gatgccgtgt gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg
     6361 cagacggtcc gtccaccgac ctcggtgaac tggcgcttgg tcagaacatg cgcgtagcgt
     6421 tcatgccgtg gaatggttac aacttcgaag actccatcct cgtatccgag cgtgttgttc
     6481 aggaagaccg tttcaccacc atccacattc aggaactggc gtgtgtgtcc cgtgacacca
     6541 agctggggcc agaagagatc accgctgaca tcccgaacgt gggtgaagct gcgctctcca
     6601 aactggatga atccggtatc gtttatattg gtgcggaagt gaccggtggc gacattctgg
     6661 ttggtaaggt tacgccgaaa ggtgaaactc agctgacccc agaagaaaaa ctgctgcgtg
     6721 cgatcttcgg tgagaaagcg tctgacgtta aagactcttc tctgcgcgta ccaaacggtg
     6781 tatccggtac ggttatcgac gttcaggtct ttactcgcga tggcgtagaa aaagacaaac
     6841 gtgcgctgga aatcgaagaa atgcagctca aacaggcgaa gaaagacctg tctgaagaac
     6901 tgcagatcct cgaagcgggt ctgttcagcc gtatccgtgc tgtgctggta gccggtggcg
     6961 ttgaagctga gaagctcgac aaattgccgc gcgatcgctg gctggagctg ggcctgaccg
     7021 acgaagagaa acaaaatcag ctggaacagc tggctgagca gtatgacgaa ctgaaacacg
     7081 agttcgagaa gaaactcgaa gcgaaacgcc gcaaaatcac ccagggcgac gatctggcac
     7141 cgggcgtgct gaagattgtt aaggtatatc tggcggttaa acgccgtatc cagcctggtg
     7201 acaagatggc aggtcgtcac ggtaacaagg gtgtaatttc taagatcaac ccgatcgaag
     7261 atatgcctta cgatgaaaac ggtacgccgg tagacatcgt actgaacccg ctgggcgtac
     7321 cgtctcgtat gaacatcggt cagatcctcg aaacccactt gggtatggct gcgaaaggta
     7381 tcggcgacaa gatcaacgcc atgctgaaac agcagcagga agtcgcgaaa ctgcgtgaat
     7441 tcatccagcg tgcgtacgat ctgggcgctg acgttcgtca gaaagttgac ctgagtacct
     7501 tcagcgatga agaagttatg cgtctggctg aaaacctgcg caaaggtatg ccaatcgcaa
     7561 cgccggtgtt cgacggtgcg aaagaagcag aaattaaaga gctgctgaaa cttggcgacc
     7621 tgccgacttc tggtcagatc cgcctgtacg acggccgcac tggtgaacag ttcgaacgtc
     7681 cggtaaccgt tggttacatg tacatgctga aactgaacca cctggtcgac gacaagatgc
     7741 acgcgcgttc caccggttct tacagcctgg ttactcagca gccgctgggt ggtaaggcac
     7801 agttcggtgg tcagcgtttc ggggagatgg aagtgtgggc gctggaagca tacggcgcag
     7861 catacaccct gcaggaaatg ctcaccgtta agtctgatga cgtgaacggt cgtactaaga
     7921 tgtataaaaa catcgtggac ggcaaccatc agatggagcc gggcatgcca gaatccttca
     7981 acgtattgtt gaaagagatt cgttcgctgg gtatcaacat cgaactggaa gacgagtaat
     8041 tctcgctcaa acaggtcact gctgtcgggg taaaacccgg cagcggattg tgctaactcc
     8101 gacgggagca aatccgtgaa agatttatta aagtttctga aagcgcagac taaaaccgaa
     8161 gagtttgatg cgatcaaaat tgctctggct tcgccagaca tgatccgttc atggtctttc
     8221 ggtgaagtta aaaagccgga aaccatcaac taccgtacgt tcaaaccaga acgtgacggc
     8281 cttttctgcg cccgtatctt tgggccggta aaagattacg agtgcctgtg cggtaagtac
     8341 aagcgcctga aacaccgtgg cgtcatctgt gagaagtgtg gcgttgaagt gacccagact
     8401 aaagtacgcc gtgagcgtat gggccacatc gaactggctt ccccgactgc gcacatctgg
     8461 ttcctgaaat cgctgccgtc ccgtatcggc ctgctgctcg atatgccgct gcgcgatatc
     8521 gaacgcgtac tgtactttga atcctatgtg gttatcgaag gcggtatgac caacctggag
     8581 cgtcagcaga tcctgactga agagcagtat ctggacgcgc tggaagagtt cggtgacgaa
     8641 ttcgacgcga aaatgggtgc ggaagcaatc caggccctgc tgaagagcat ggatctggag
     8701 caagagtgcg aacagctgcg tgaagagctg aacgaaacca actccgaaac caagcgtaaa
     8761 aagctgacca agcgtatcaa actgctggaa gcgttcgttc agtctggtaa caaaccagag
     8821 tggatgatcc tgaccgttct gccggtactg ccgccagatc tgcgtccgct ggttccgctg
     8881 gatggtggtc gtttcgcgac ttctgacctg aacgatctgt atcgtcgcgt cattaaccgt
     8941 aacaaccgtc tgaaacgtct gctggatctg gctgcgccgg acatcatcgt acgtaacgaa
     9001 aaacgtatgc tgcaggaagc ggtagacgcc ctgctggata acggtcgtcg cggtcgtgcg
     9061 atcaccggtt ctaacaagcg tcctctgaaa tctttggccg acatgatcaa aggtaaacag
     9121 ggtcgtttcc gtcagaacct gctcggtaag cgtgttgact actccggtcg ttctgtaatc
     9181 accgtgggtc catacctgcg tctgcatcag tgcggtctgc cgaagaaaat ggcgctggag
     9241 ctgttcaaac cgttcatcta cggcaagctg gaactgcgtg gtcttgctac caccattaaa
     9301 gctgcgaaga aaatggttga gcgcgaagaa gctgtcgttt gggatatcct ggacgaagtt
     9361 atccgcgaac acccggtact gctgaaccgt gcaccgactc tgcaccgtct gggtatccag
     9421 gcatttgaac cggtactgat cgaaggtaaa gctatccagc tgcacccgct ggtttgtgcg
     9481 gcatataacg ccgacttcga tggtgaccag atggctgttc acgtaccgct gacgctggaa
     9541 gcccagctgg aagcgcgtgc gctgatgatg tctaccaaca acatcctgtc cccggcgaac
     9601 ggcgaaccaa tcatcgttcc gtctcaggac gttgtactgg gtctgtacta catgacccgt
     9661 gactgtgtta acgccaaagg cgaaggcatg gtgctgactg gcccgaaaga agcagaacgt
     9721 ctgtatcgct ctggtctggc ttctctgcat gcgcgcgtta aagtgcgtat caccgagtat
     9781 gaaaaagatg ctaacggtga attagtagcg aaaaccagcc tgaaagacac gactgttggc
     9841 cgtgccattc tgtggatgat tgtaccgaaa ggtctgcctt actccatcgt caaccaggcg
     9901 ctgggtaaaa aagcaatctc caaaatgctg aacacctgct accgcattct cggtctgaaa
     9961 ccgaccgtta tttttgcgga ccagatcatg tacaccggct ttgcatatgc agcgcgttct
    10021 ggtgcatctg ttggtatcga tgacatggtc atcccggaga agaaacacga aatcatctcc
    10081 gaggcagaag cagaagttgc tgaaattcag gagcagttcc agtctggtct ggtaactgcg
    10141 ggcgaacgct acaacaaagt tatcgatatc tgggctgcgg cgaacgatcg tgtatccaaa
    10201 gcgatgatgg ataacctgca aactgaaacc gttattaacc gtgacggtca ggaagagaag
    10261 caggtttcct tcaacagcat ctacatgatg gccgactccg gtgcgcgtgg ttctgcggca
    10321 cagattcgtc agcttgctgg tatgcgtggt ctgatggcga agccggatgg ctccatcatc
    10381 gaaacgccaa tcaccgcgaa cttccgtgaa ggtctgaacg tactccagta cttcatctcc
    10441 acccacggtg ctcgtaaagg tctggcggat accgcactga aaactgcgaa ctccggttac
    10501 ctgactcgtc gtctggttga cgtggcgcag gacctggtgg ttaccgaaga cgattgtggt
    10561 acccatgaag gtatcatgat gactccggtt atcgagggtg gtgacgttaa agagccgctg
    10621 cgcgatcgcg tattgggtcg tgtaactgct gaagacgttc tgaagccggg tactgctgat
    10681 atcctcgttc cgcgcaacac gctgctgcac gaacagtggt gtgacctgct ggaagagaac
    10741 tctgtcgacg cggttaaagt acgttctgtt gtatcttgtg acaccgactt tggtgtatgt
    10801 gcgcactgct acggtcgtga cctggcgcgt ggccacatca tcaacaaggg tgaagcaatc
    10861 ggtgttatcg cggcacagtc catcggtgaa ccgggtacac agctgaccat gcgtacgttc
    10921 cacatcggtg gtgcggcatc tcgtgcggct gctgaatcca gcatccaggt gaaaaacaaa
    10981 ggtagcatca agctcagcaa cgtgaagtcg gttgtgaact ccagcggtaa actggttatc
    11041 acttcccgta acaccgaact gaaactgatc gacgaattcg gtcgtaccaa agaaagctac
    11101 aaagtacctt acggtgcggt actggcaaaa ggcgatggcg aacaggttgc tggcggcgaa
    11161 accgttgcaa actgggaccc gcacaccatg ccggttatca ccgaagtaag cggttttgta
    11221 cgctttactg acatgatcga cggccagacc attactcgtc agaccgacga actgaccggt
    11281 ctgtcttcgc tggtggttct ggattccgca gaacgtaccg caggtggtaa agatctgcgt
    11341 ccggcactga aaatcgttga tgctcagggt aacgacgttc tgatcccagg taccgatatg
    11401 cctgcgcagt acttcctgcc gggtaaagcg attgttcagc tggaagatgg cgtacagatc
    11461 agctctggtg acaccctggc gcgtattccg caggaatccg gcggtaccaa ggacatcacc
    11521 ggtggtctgc cacgcgttgc ggacctgttc gaagcacgtc gtccgaaaga gccggcaatc
    11581 ctggctgaaa tcagcggtat cgtttccttc ggtaaagaaa ccaaaggtaa acgtcgtctg
    11641 gttatcaccc cggtagacgg tagcgatccg tacgaagaga tgattccgaa atggcgtcag
    11701 ctcaacgtgt tcgaaggtga acgtgtagaa cgtggtgacg taatttccga cggtccggaa
    11761 gcgccgcacg acattctgcg tctgcgtggt gttcatgctg tgactcgtta catcgttaac
    11821 gaagtacagg acgtataccg tctgcagggc gttaagatta acgataaaca catcgaagtt
    11881 atcgttcgtc agatgctgcg taaagctacc atcgttaacg cgggcagctc cgacttcctg
    11941 gaaggcgaac aagttgaata ctctcgcgtc aagatcgcaa accgcgaact ggaagcgaac
    12001 ggcaaagtgg gtgcaactta ctcccgcgat ctgctgggta tcaccaaagc gtctctggca
    12061 accgagtcct tcatctccgc ggcatcgttc caggagacca ctcgcgtgct gaccgaagca
    12121 gccgttgcgg gcaaacgcga cgaactgcgc ggcctgaaag agaacgttat cgtgggtcgt
    12181 ctgatcccgg caggtaccgg ttacgcgtac caccaggatc gtatgcgtcg ccgtgctgcg
    12241 ggtgaagctc cggctgcacc gcaggtgact gcagaagacg catctgccag cctggcagaa
    12301 ctgctgaacg caggtctggg cggttctgat aacgagtaat cgttaatccg caaataacgt
    12361 aaaaacccgc ttcggcgggt ttttttatgg ggggagttta gggaaagagc atttgtcaga
    12421 atatttaagg aatttctgaa tactcataat caatgtagag atgactaata tcctgaaact
    12481 gactgaacta attgagtcaa actcggcaag gattcgatac tattcctgtg tagctttctt
    12541 aaggaacgag aatgaaacag gaagtggaga agtggcgacc ttttaaacat ccggatggcg
    12601 acgttcgtga tttatcgttt cttgatgctc atcaggttat ctacgttcag catcatgagg
    12661 acaaggagcc tttagagtat cgcttttggg ttacctactc tcttcactgc ttcacaaaag
    12721 attatgaaca tcagacgaac gaagaaaaac tatcgttaat gtaccacgcg cctaaagaat
    12781 ctcgtccctt ctgccagcac cgttataact tagcgcgcac acacttaaaa agaactattt
    12841 tggcgctgcc agaaagcaac gttattcatg ccgggtatgg tagctatgcc gtgattgagg
    12901 tggacttaga cggaggagat aaggcatttt actttgttgt gttcagggct ttcagtgaaa
    12961 tttttcacca ttgcctaaaa cttattgaga aataagcagc ttcctcagcc ctcaaaataa
    13021 caaaacccac cttaaggtgg gtttcgccag agaattatct ctggtattca gaacgccatt
    13081 accggacttt gccttgacct tgcgataatc gcaggttgcg ggatgtctga atttcttcag
    13141 tctgctgcat cctggaagat gagaacatgt gttcttattt tcgtctctat catagttgag
    13201 tatttactct cttacaatca gatctctttc attgctcaac aggcgatggc ttcagacttt
    13261 gcattacgga atttttaaga aaggcagggc gaaacgagga agaagctttt cgccctggac
    13321 aacattcctg ctgacggcac taccataaaa aaacacaacg ctttcaacaa ccttgcgttt
    13381 ttcatcttcc gtagcggctc ggaataaatt ttcgttctta cattccgtct catagtcttt
    13441 gcgaggcgcg tcccagatag ctgtcccagt ctttccatac cggctgcaaa ccctgagcgg
    13501 ttaacgcggc agcaaccgct tccggtctgc gctcgtcgtg cggtgagaac tgttccagct
    13561 cggggtgatt gtcggcatag ccacctggct gcgttttcga aaaagcgctg acgttattaa
    13621 ttgccagcgg aataacgcga tcgcgaaacc acggtgattc ccgcgtggag agtgacagtt
    13681 caatctccgg tgcaagcagc cggaaggcgc agatggtttg cactaactgg cgttcatcca
    13741 taatcgacgc aggctcaatg ccgccagtac atggacgcag gcgtgggaag gagacagagt
    13801 agcggctttg ccagtaatgc tgttgcagcc atagcaaatg ttctgcaacc atatagcagt
    13861 caacgcgcca gctgtcggaa aggccaatta gcgcgccgag gcctatctta tcaatccccg
    13921 cacggcccag ccgatccggc gtttccagcc gccagaagaa gtcctgtttt ttgcctttca
    13981 gatgatggcg ggcataagtc gcctcgtgat atgtctcctg ataaaccatc acgccatcca
    14041 gaccaagttg ctttaactcg gcgtattccg tctccgccag cggctgtact tccatctgta
    14101 gtgaagagaa ctgttcacgc agggcaggga gatgacgacg aaagtaatcc atccccactt
    14161 tcgcctgatg ttcaccggtg actaaaagca gatgttcaaa gcccatctcc cgtatagcgg
    14221 cgctttccct ggcaatatcc gcttcatcca gcgttttgcg cttgatgcgg ttgctcatag
    14281 agaatccgca gtacgtgcag tcgttagcgc aaagattgga aagataaagc gggacgtaga
    14341 aactaactgt gttgccaaat cgctgacggg tcagacgctg cgcccgttgg gccagttgtt
    14401 ccagatagcc actggcggct ggcgataaca gcgccatcat atcgtcgcgg gtgagttgcg
    14461 aggcatttag cgcccgctct acgtcagcag ccgttttgcc gttgatacgc aggcggatgt
    14521 cgtcccagtc cagttgtcgc cagcgatcgc tgaaggtttt cataccgatg cctccagaaa
    14581 tccggtcagc gggctggtgg catgagcaaa ataactgcgg ctgcccggtc cggactgagg
    14641 tgccagcagg cctgcttcta ctgccagacg aaatgccttc gccatgttga cgggatcgtc
    14701 cgcgacggca atcgccgtat tcactaacac cgcgtcggcc cccatttcca gcgcctgcgc
    14761 ggcatggctg ggaacgccga tgccagcatc gacaaccacc ggaactgtgg cctgctggat
    14821 aataatctcc agcatggcgc gggtttccag cccctgattc gagccaatcg gcgcgccgag
    14881 cggcatcacc gctgcacagc cgacttcttc cagacgttta cacaataccg gatcggcccc
    14941 gcagtaaggc agcacgacaa atccctgttg taccagcgtt tcggcggctt tcagggtttc
    15001 gatgggatcg ggcaacagcc agcgggcgtc agggtgaatc tccaatttta accagtttgt
    15061 gcctaacgct tcacgagcca gatgggcggc gaaaatggct tcttccgctg ttttcgcccc
    15121 ggatgtattt ggcagcaggg tcacacccgc cgcgataagc ggttcgagga tagcgtcgtt
    15181 gtgctggcgc aagtcgacac gtttcatcgc cagcgtcacc agctggctgc cggaagcgcg
    15241 gattgcctct accatcagtt gtgaagaggc gaatttcccg gtgccggtaa acagatgtga
    15301 atcaaacgtt ttgtccgcaa tacgtaacat ctcaaccccc tgcaataacc tgaaaaagta
    15361 ggatctggtc gccatcctgc acgatatgtt gcgtccactg ctcacgcggg acgatttgct
    15421 gattaatcgc cagagccgcg cccgcttgtc gttgaacgag ttgctccagt agttcgtgaa
    15481 cagtttgccc ggcggcgcac tgcatcggtt gatcgttaaa caggatctgc attgcttcct
    15541 ccgcataccg ggcaaccact ggcgcggcgc aacgccaggc tgcgccactg gctcgactta
    15601 ccgtcgaaca gtcggagttc tcccgcaggc gtctctatac cgcttaataa cttaatggct
    15661 tccagtgcct gcaaagtgcc cataaccccg accaccgggc caaccacgcc cgccgtgcgg
    15721 cagttgcgtt ctggctcctg gttttctggc cacaggcagc ggtaacaacc ctgcccccag
    15781 ggcggcgtca gtaccatcaa ctgaccgcca aatccgaccg cgctggcggt gataagcggc
    15841 gtgttgagtg ccacgcaggc ggcattaatc tcctggcgag tcgccatatt gtcggtacag
    15901 tcgagcacca catcggcttg tgcaaccgca tcttttaacg cctcacccgt taaccgttgt
    15961 tgtaatgctg tcagttgaat atcgggattc aactgtgtca gtcgctgatg gctgacctgc
    16021 gatttcgggc gatcgatatc ttcagtggta aagaggattt gtcgttgcag attgcttaaa
    16081 tgcacatcgt cgtcatctgc cagtaccagc gtcccgacgc cagcgcccgc caggtatagc
    16141 gcagcaggtg tacccagccc gccaagaccg ataatcagca cctggctgtc gagcagtttt
    16201 tgctgcccgt caagagcgat atcgtcgagc aggatttggc ggctataacg cataaaatca
    16261 cggtcattca tcgccaactc ctgcaatttc cagcaactgt gccgttgcca aacgccagtc
    16321 tgcggcttga gtaatggcgc tgacgacggc gatactgccg acacccgttg ctatcaccgc
    16381 aggcgcgcgt gccagactga taccgccaat cgccacggtg ggataatccg acagtcgctc
    16441 aacatgccgt gccagctgtt ccagcccctg cggtgcagaa ggcatctgtt tggtttgcgt
    16501 cgggaacaca tgtcccagcg cgatataaga ggggcgtgct gccagcgcga cgtcgatttc
    16561 catatcgtca tgggtcgaaa cgcccagccg caggcctgcc gcgcggatgg cattgagatc
    16621 ggtggcttgc aaatcttcct gccccaaatg gacgccatac gcctgatgct tgatcgcgag
    16681 ccgccagtaa tcgttgataa acaatcgcgc gttatagcgg cggcccagcg caattgccgc
    16741 cacgacatcg gcttccacct cttcatcgcg ccgatctttg atgcgtagct ggagagtacg
    16801 tacgcctgca tccaacagac gttcgatcca ctgtacgctg tccaccaccg ggtacagtcc
    16861 taaacgaaaa ggtacaggag gaaaatcagg ctgatacatc acgcttcctc cttacgcagg
    16921 tagatttctc cgcctctggc acggaagttc tccgacatat ccgctattcc catttcaata
    16981 gtttgcgtgg cggcgtaatc acgcacttcc tggctgattt tcatcgagca gaatttcggc
    17041 ccacacatgg agcaaaaatg ggcgacttta cctgactctt gcggcagggt ttcatcgtga
    17101 taagcgcggg cggtaaacgg gtcgagggcc agattaaact ggtcttccca gcgaaattcg
    17161 aagcgggctt tcgacatggc gttatcgcga atttgtgcgc ccggatgccc tttcgccagg
    17221 tcagcggcgt gggcggcaat cttataggtg ataagcccct gcttaacatc ttctttatta
    17281 ggcagaccca gatgctcttt tggcgttacg taacagagca tcgcgcagcc aaaccagcca
    17341 atcatcgccg caccaatccc cgacgtgaag tggtcatagc ccggcgcaat atcggtagtt
    17401 agcggcccca gagtgtaaaa cggcgcttcg tggcagtgct ctaactcctc ggtcatattg
    17461 cggcggatca tctgcatcgg cacgtggcct gggccttcaa tcatcacctg cacatcatat
    17521 tcccaggcga tttttgtcag ttcgcccagc gtatgcagct cggcaaactg cgcttcatcg
    17581 ttggcgtcct gaatagaacc ggggcgcaga ccgtcgccca gcgacagcga aacgtcataa
    17641 gcggcacaga tttcacaaat ttcgcggaag tgttgataga ggaaattttc ctgatgatgg
    17701 gagaggcacc atttcgccat aatcgaaccg ccgcgcgaga cgataccggt caggcgtttc
    17761 gcggtcatcg gcacatagcg cagcagcacc cccgcatgga tagtgaagta atccacacct
    17821 tgctcggcct gttccagcag cgtgtcgcgg aacgcttccc aggtaagatc ttcggcgatc
    17881 ccattaacct tctccagcgc ctggtagatc ggcactgtac caatcggcac cgggctgtta
    17941 cgcaaaatcc actcgcgggt ttcgtgaata tagcgaccgg tggagagatc catcaccgta
    18001 tccgctcccc agcgcgtgga ccataccagc ttttccactt cttcttcgat ggaagaggtg
    18061 accgccgagt tgccgatatt ggcgttaact tttaccagga aattgcgacc aataatcatc
    18121 ggctccgatt ccggatgatt aatgttggcc gggataatcg cacgtccggc agcaacttca
    18181 tcacggacaa attccgcagt gatattttcc ggcagacgtg cgccaaagct cattcccgga
    18241 tgctggtggc gtaaaacttc gctacggatg cgctcgcggc ccatattctc gcggatggcg
    18301 atgaattcca tttccggtgt gatgatgccc tggcgggcgt agtgcagttg tgtgacgcgg
    18361 cgtcctgctt tggcgcgttt tggcgttaat acgccgctaa aacgcagctc gtcgaggcca
    18421 tcatctgcca gccgcgcttt agtgtaatcg gaactgcgca cggtaagttc ttcggtatcg
    18481 ccgcgcgcat cgatccacgg ctggcgtagt tttgccagcc cttgctgcac gttaatggcg
    18541 atctgcggat cgccatacgg gccggaggtg tcgtagaccg gaatcgcttc gttttcttcg
    18601 tactgcggct gttctttgct accgccaatt agcgtcgggc taagctggat ctcacgcatc
    18661 ggcacgcgca cgccgggttg tgtgccagtg atataaatgc gttttgagtt gggaaaggcg
    18721 gtgccttcca gggtgtcgat aaaatgttgg gcctgggcgc gttgttcgcg acgggtcagt
    18781 tttgttgcag acatagctca ttccaaaaag ttaaggacgt ggcttgtcag acgacggatg
    18841 aagcaagaga cgatcgccgc aaaggcgatg cgatagcaga ttaactcttg ttcccttcgc
    18901 aggtattagc ctgatcaggt tccgcggatc ccgaataaac ggtctcagcc agttaaggca
    18961 ctccgacaag aaattagccc cacaaacggg gcgttgaatg taaattacgc gttaacagcg
    19021 cagaactcaa gcaggatgtt tgacgcgggc ggcgtcaagc accagcagaa tcagcttatc
    19081 ttccagcaca aagcgcgctt ccagcgcctc gccgatgtca gataaaacct gttgaaactc
    19141 aaggtaatta tcatgatcga tagcggtttc caggctggaa tcgtagtaat ccataatctg
    19201 ttgggtattg gcttcgagtt gcggccaaat cttcgcggcg cgcgcgagtt gcccgttgcc
    19261 ttccagctta tgaagaatgc gctcataaat actgaaatgt ccggcagaca agtaatcgac
    19321 caggctctga caaaaatcat caagggcttt ttcgtttagc ctcatgtacg attctttgcc
    19381 aggcttaatg ccaaccagat tgtagtaagc cacgagcaga tgcttacgta catgtagcca
    19441 gcgatcaacc agtttgttac ttcctctgac gcgctccgtc aggttatcga gctggttaag
    19501 catgattgac tccgcaagtt tgtattcaaa aactgctcag tgagaaatgt aaaaaccatg
    19561 ttaaacatgc cagtgatgca aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt
    19621 agatcacggc tggtgggtcg tcagccatga acaaaaatta tggttgccga agggagaatt
    19681 gccatatggc gaagcggcaa atttcgatct tgtgggtcag cgcgcactac aaatcggcga
    19741 atggcagggg gaacctgttt ggttaataca acagcagcgg cgttacgata tggggtcggt
    19801 acgtcaggtc attgatctcg atgttgggct gtttcaactg gccggacgtg gcgtacaact
    19861 ggcggagttt taccgatcgc ataaatactg tggttactgc gggcatgaaa tgtatccaag
    19921 caaaaccgaa tgggcgatgc tgtgcagcca ttgccgtgag cgttactacc cgcaaatcgc
    19981 cccctgcatt attgttgcca tccgtcgcga tgattcgatc ctcctcgccc agcatacccg
    20041 ccatcgtaac ggtgtccata cggtacttgc cggattcgtc gaagtgggcg aaaccctcga
    20101 gcaggcggtc gcgcgggaag tgatggaaga gagcggaatt aaagttaaac acttgcgtta
    20161 tgtgacttcc cagccgtggc cgtttcctca gtctttaatg accgcgttta tggcggaata
    20221 tgacagcggc gacatcgtga tcgacccgaa agaattgctc gaggcgaact ggtatcgcta
    20281 tgacgatttg ccgttactcc cgccgcccgg caccgtagcg cgccgtctga tagaagatac
    20341 ggtggcgatg tgtcgggcag agtatgagtg atgatacact gaccgcctga cgcactaagg
    20401 aacagccaaa atgaccgaac ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc
    20461 cgttgatgtc actcctgtat ggatgatgcg ccaggcgggt cgctatctac cggaatataa
    20521 agccacgcgc gcccaggcgg gcgattttat gtcgctgtgc aaaaacgccg agctggcgtg
    20581 tgaagtgacc ttgcaaccgc tgcgtcgcta cccgctggat gcggcgatcc tcttttccga
    20641 tatcctcacc gtcccggacg cgatggggtt agggctctat tttgaagccg gagaaggtcc
    20701 gcgttttacc tcgccagtca cctgcaaagc tgacgtcgat aaactgccaa ttccggaccc
    20761 ggaagatgaa ctggggtacg tgatgaacgc ggtgcgtacc attcgtcgcg aactgaaagg
    20821 cgaagtgccg ctgattggtt tttccggcag cccgtggacg ctggcgacct acatggtgga
    20881 aggcggcagc agcaaagcct tcaccgtgat caaaaaaatg atgtatgccg atccgcaggc
    20941 gctgcacgct ctactcgata aactggcgaa aagcgtcgct ttgtatctga atgcgcagat
    21001 taaagccggt gctcaggctg tgatgatttt cgacacctgg ggcggcgtgc ttaccgggcg
    21061 cgattatcaa cagttctcgc tttattacat gcataaaatt gttgatggtt tactgcgtga
    21121 aaacgacggt cgccgcgtac cggtcacgct gtttaccaaa ggcggcggac agtggctgga
    21181 agccatggca gaaaccggtt gcgatgcgct gggcctcgac tggacaacgg acatcgccga
    21241 tgcgcgccgc cgtgtgggca ataaagtcgc gttgcagggt aatatggatc cgtcgatgct
    21301 gtacgcgccg cctgcccgca ttgaagaaga agtagcgact atacttgcag gtttcggtca
    21361 cggcgaaggt catgtcttta accttggtca cggcattcat caggatgtgc cgccagaaca
    21421 tgctggcgtg ttcgtggagg cagtgcatcg actgtctgaa cagtatcacc gctaaggagc
    21481 gattatggat ctcgcgtcat tacgcgctca acaaatcgaa ctggcttctt ctgtgatccg
    21541 cgaggatcga ctcgataagg atccgccgga tctgatcgcc ggagccgatg tcgggtttga
    21601 gcagggcgga gaagtgacgc gagcggcgat ggtgctgctg aaatacccct cgcttgagct
    21661 ggtggagtat aaagtggccc gcatcgctac caccatgcct tacattccag gttttctttc
    21721 cttccgcgaa tatcctgcgc tgctggcagc gtgggagatg ctgtcacaaa agccggattt
    21781 agtgtttgtc gatggtcatg ggatctcgca tcctcgccgt cttggcgtcg ccagccattt
    21841 tggtttgctg gtggatgtgc cgaccattgg cgtagcgaaa aaacgactat gcggtaaatt
    21901 cgaaccgctc tccagcgaac cgggcgcgct ggccccactg atggataaag gcgagcagct
    21961 ggcctgggtc tggcgcagca aagcgcgctg taacccgcta tttatcgcta ctggtcatcg
    22021 ggtcagcgtg gacagcgcgc tggcgtgggt acaacgctgc atgaaaggct atcgtctgcc
    22081 ggagccaacg cgctgggcgg acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac
    22141 agcaaatcag ccctaattca ggtaaactgc ggataatttc cgtatttgag aactcaacat
    22201 gttacaaaac ccaattcatc tgcgtctgga acgcctggaa agctggcagc atgtcacttt
    22261 catggcttgc ttatgcgaac gcatgtaccc caattacgcc atgttctgcc agcaaaccgg
    22321 ttttggtgat gggcaaattt accgtcgtat tctcgatctc atctgggaaa cgctgaccgt
    22381 taaagatgca aaagtaaatt tcgacagcca actggagaaa tttgaagaag cgattccttc
    22441 agccgacgat ttcgatctgt acggcgttta tccggcaatc gatgcctgcg tggcgttaag
    22501 tgaactggtc cattcgcgtt tgagtggtga aacgctcgaa cacgcggtgg aagtgagtaa
    22561 gacctccatc acgaccgttg cgatgctgga aatgactcag gctggtcgcg aaatgagcga
    22621 tgaagagctc aaagaaaacc cagctgtaga gcaagaatgg gacattcagt gggaaatatt
    22681 ccgactttta gctgagtgcg aagaacgcga tatcgagctg ataaaaggcc ttagggcaga
    22741 cctgcgtgag gcggatgaga gcaatattgg tataattttt cagcaataag accagaaaac
    22801 gtgatttaac gcctgatttg tcgtacctgg agtcttccct ttcgcccccc gtctggtcta
    22861 catttggggg gcgaaaaaaa gtggctatcg gtgcgtgtat gcaggagagt gctattctgg
    22921 catttccgtc gcactcgatg cttagcaagc gataaacaca ttgtaaggat aacttatgaa
    22981 caagactcaa ctgattgatg taattgcaga gaaagcagaa ctgtccaaaa cccaggctaa
    23041 agctgctctg gagtccactc tggctgcaat tactgagtct ctgaaagaag gcgatgctgt
    23101 acaactggtt ggtttcggta ccttcaaagt gaaccaccgc gctgagcgta ctggccgcaa
    23161 cccgcagacc ggtaaagaaa tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg
    23221 caaggcactg aaagacgcag ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg
    23281 ggtggtttca ccccttttgt ctttctggcg tcgatcattg atgctggctg gcgctctgtt
    23341 tctcactgct tgtagtcaca actcttcact tcctcccttt accgccagtg gatttgctga
    23401 agaccagggc gcggtacgca tctggcgaaa agacagcggc gataatgtgc atcttcttgc
    23461 cgtgtttagc ccgtggcgca gtggcgatac cacgacgcga gagtatcgct ggcagggcga
    23521 taacctcacg ctcatcaata tcaatgttta cagcaaaccc ccggtgaata ttcgcgcgcg
    23581 ttttgacgat cgcggtgatc tgagctttat gcaacgtgaa tccgatgggg aaaagcagca
    23641 gctttctaac gaccaaatcg atttataccg ttatcgggct gatcagatcc gccagattag
    23701 cgatgcctta cgtcagggga gagtcgtgct gcgccagggg cgctggcatg cgatggaaca
    23761 gaccgtgacc acctgcgaag ggcaaaccat taaacctgat ttagattcgc aggcaatagc
    23821 gcatatcgag cgccgccaga gccgctcttc tgttgatgtc agcgtggcat ggctggaagc
    23881 gcccgaaggt tcgcaattac tgttagtggc aaactctgat ttctgtcgct ggcaacccaa
    23941 cgagaaaacg ttctgattta ccagtggccc atacccatat gaccgccacc accgcagccg
    24001 ccgtagccca tgcccattcc ggctccgcgc ggaatacccg cttcagccat cgcaatatcc
    24061 cgtttcaccc gtaactcatc taacgactga cgcaaattct ccatctcttt ggcgaccgcg
    24121 ttaattttgc tgctatccgg tgggttcgcg gctaacaggg cattgtattc ataacgcttc
    24181 gtcaccagtt gctgttgcag tgcgctgctt tgagcgtaaa agtcattatg gattttctgc
    24241 cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt gctgccacat accgtgtccg
    24301 ccgtgagcaa atgcagatgt cgatcccatc gccattgctg aaagcgccat cattaccagg
    24361 gcaattttcg tgttccgttt catggttaat cctccagtgg ttgtcttact tcgggtattg
    24421 catcttccgt gccaacgatg aaacgctgat atgacgggta atctggcatg ataaacgagt
    24481 aaaaatgact cgcctgctgc gggtagcgag tcatttttac tcattgaaac ttaagccttt
    24541 gtgttacagc gcagggtaag cgctgataaa agatggcatg atttctgctg tcagaaaggg
    24601 atgagcaggc aaagaagaag atgcgtttta tgcaacgttc taaagactcc ttagctaaat
    24661 ggttaagcgc gatcctcccc gtggtcattg ttgggctggt agggttgttt gcggtgacgg
    24721 tgattcgcga ttatggccgc gagactgccg ccgccagaca aacgctgctg gaaaaaggca
    24781 gtgtacttat tcgtgctctc gaatccggct cgcgcgtcgg catggggatg cgcatgcatc
    24841 atgcgcagca gcaggcctta ctggaagaaa tggccgggca gcctggagta cgttggtttg
    24901 cggtcacgga tgaacaagga acaatcgtga tgcatagcaa ctccggcatg gtgggaaaac
    24961 agctttattc cccgcaggaa atgcagcagt tacatccggg agatgaagaa gtgtggcggc
    25021 ggatcgatag cgcagacggc gagcctgttc tggaaattta tcgccagttt caaccgatgt
    25081 ttgctgctgg aatgcaccgg atgcgccata tgcagcagta tgccgcgaca ccacaagcaa
    25141 ttttcatcgc tttcgacgcc agtaatattg tgagtgccga agatcgtgag cagagaaaca
    25201 ccctgattat cctcttcgcc ctggcgacgg tcttgctggc aagcgtattg tcattcttct
    25261 ggtatcgccg ctatctgcgc tcgcgccagc ttctacaaga tgaaatgaag cgcaaagaga
    25321 agctggtggc gctggggcat cttgcggcag gcgttgccca cgaaattcgt aacccgcttt
    25381 cctcgattaa agggctggca aaatactttg ccgagcgcgc gccagcaggg ggagaagcgc
    25441 atcaactggc gcaggtgatg gcgaaagaag ctgaccgttt aaaccgcgtg gtaagcgagc
    25501 tgctggaact ggttaagcca acgcatctgg ctttgcaggc ggtggatctc aacacgctga
    25561 ttaaccactc attacagctg gtaagccagg atgcaaactg ccgggagatc cagttacgct
    25621 ttaccgccaa cgacacgttg ccggaaattc aggccgatcc ggacaggctg actcaggtcc
    25681 tgttgaatct ttatctcaat gctattcagg cgattggtca gcatggcgtg attagcgtga
    25741 cggccagcga aagcggcgcg ggcgtgaaaa tcagcgttac cgacagcggt aagggaattg
    25801 cggcagatca gcttgaagcc atcttcaccc cgtacttcac caccaaagcc gaaggcaccg
    25861 gactggggct ggcggtcgtg cataatattg ttgaacaaca cggtggtaca attcaggtcg
    25921 caagcctgga gggaaaaggc gcaaggttca ccctctggct tccggtcaat attacgcgta
    25981 aggacccaca aggatgacgc acgataatat cgatattctg gtggtggatg atgacattag
    26041 ccactgcact attttgcagg ctttgctgcg cggctggggc tataacgtcg cgctggcgaa
    26101 cagcgggcga caggcgctgg agcaggtgcg ggagcgggtt tttgatcttg tgctttgcga
    26161 tgtgcgaatg gcggagatgg acggcattgc cacgctaaaa gagatcaaag cgttaaatcc
    26221 ggcgattccg gtactgatta tgaccgcata ctctagcgtc gagacggcgg tagaggcgtt
    26281 gaaaaccggg gcgctggatt atctcatcaa gccgctggat ttcgataacc tgcaggcgac
    26341 gctggaaaag gcgctcgcgc atacgcacat tattgatgct gaaacgcctg cggtgactgc
    26401 cagccagttc ggtatggtcg gtaaaagccc ggcgatgcaa cacctgctca gtgaaatcgc
    26461 cctcgtcgcg ccatcggaag ccacggtgct gatccacggc gattccggca ccggtaaaga
    26521 gctggtcgcc agggcgattc acgccagtag cgcacgtagc gaaaaaccgc tggtaacgct
    26581 caactgtgcg gcactcaacg aatccttgct ggaatctgaa ttgttcggtc acgaaaaagg
    26641 ggcgtttact ggagccgata aacgccggga agggcgcttt gttgaggcgg acggcggcac
    26701 gttgtttctc gatgaaattg gcgatatctc gccgatgatg ctggtgcgtc tgctgcgtgc
    26761 gattcaggag cgcgaagttc agcgtgtcgg tagcaaccag actatttcgg ttgatgtccg
    26821 gctgattgcc gcgacccatc gcgatcttgc cgcagaggtg aatgccgggc gttttcgcca
    26881 ggatctctac tatcgcctga acgtggtggc gattgaagta ccatcgctgc ggcaacggcg
    26941 ggaagatatt cctctgctgg ctggccattt tctgcagcgc tttgctgaac gtaatcgcaa
    27001 ggcggtaaaa ggctttacgc cccaggcgat ggatctgttg attcattacg actggccggg
    27061 aaatattcgt gaactggaaa atgcggtgga acgggcggtg gtgctgctga ctggggaata
    27121 tatttccgaa cgcgagctgc cgctggcgat tgccagtacg ccgatcccgc tggcacaaag
    27181 cctggatatt cagccgctgg tggaagtcga aaaagaagtg attctggcgg cactggagaa
    27241 aacgggcggc aacaaaaccg aagccgcccg tcagttaggg atcacgcgca aaacgctgtt
    27301 ggcaaaattg tcgcgttagt tctgctcgcg ttcgatagcg cgccagccga tatctttccg
    27361 gcagaagcag tcgtcccagt ggatatcggt cattaaggca taggcgcgtt tctgtgcttc
    27421 tgctacggta tgacccagcg cggtgacgca cagtacgcgc ccgccgctgg ttaccacctg
    27481 ctcgtcatcc gccagttttg tgcccgcgtg gaacactttg ccgtcttcca cttcttccag
    27541 cggcaggccg tggatcacgt caccggtgcg gtaatcgccc ggatatccac ccgcagccat
    27601 caccacgccg agagaagcac gttcatccca ctctgacgtt ttctcgtcta gtttgccttc
    27661 acaggccgcc aggcagagtt caacaagatc ggacttcatg cgcagcataa tcggctgggt
    27721 ttctggatcg ccaaagcggc agttaaattc gataaccttc ggattgccct gtttgtcgat
    27781 catcaggccc gcgtagagaa aaccggtgta ggtgttgcct tccgacgcca tgcctttcac
    27841 ggttggccag atgatacgtt ccatcgtgcg ctgatgaacg tcatctgtca ctaccggcgc
    27901 aggagagtaa gcgcccattc cgcccgtgtt cggcccggta tctttatcgc ctacgcgttt
    27961 gtgatcctgg ctggtggcca tcggcagcac atgctcgccg tccaccatca cgataaagct
    28021 cgcttcttcg ccgtcgagga actcttcgat aacgatgcga tgacccgcgt cgccaaacgc
    28081 gttgcccgcc agcatatcgt gaacagccgc ttccgcttct tccagcgtca tcgccacgat
    28141 aacgcctttc ccggcagcca gaccgtccgc tttaatgacg attggcgcgc ctttctcacg
    28201 cagatacgcc agcgcaggtt ctacctcggt gaagttctga tattccgccg taggaatgtt
    28261 atggcgggcc aggaaatctt tagtaaacgc tttcgagcct tccagctgtg cggcgcctgc
    28321 ggttgggccg aagattttca tcccggcggc gcggaaggta tcgaccacgc ctttcaccag
    28381 cggcgcttcc gggccgacga tggttagatc taccttttcg ttttgcgcga aatccagcag
    28441 cgccgggata tcggtcacgc cgatagcgac gttttgcagc gtgggttcca gcgctgtgcc
    28501 tgcgttaccc ggagcaacaa aaacagtctc aaccagcggc gactgggccg ctttccaggc
    28561 cagcgcgtgc tcgcgcccgc cgttaccaat cactaatact ttcatctgtc gctccattaa
    28621 tggcggaagt ggcgcatatc ggtgaagagc atcgcaatac cgtgctcgtc ggcggcggca
    28681 atcacttcgt catcacggat ggaaccgcca ggctggatta cgcaggtcac gcccgcagcg
    28741 gcggcggcat caataccgtc gcggaacggg aagaacgcgt cagaagccat cgaggaacct
    28801 ttcacttcca ggccttcatc ggcagcttta ataccggcga ttttcgcgga gtacacgcgg
    28861 ctcatctggc ccgcgccaat accgatggtc atattgtttt tggcatagac gatagcattg
    28921 gatttcacga acttcgccac tttccagcag aacagcgcat cacgcagttc ctgttcggtc
    28981 ggctgacgtt tggtgacgac gcgcagttct tccgcaccga ccatgcccag atcgcgatcc
    29041 tgaaccagca gaccgccgtt cacgcgtttg aaatcaagac ccggaacacg ctcgccccac
    29101 tgaccgcagg tcagaacgcg tacattctgt ttggcggcgg tgattttcag ggcttcttcg
    29161 ctggcggacg gcgcaataat cacttcaaca aactgacgag aaatgatggc ctgtgcggtt
    29221 tccgcatcca gctcgcggtt aaaggcaatg atgccgccga atgcggaggt cgggtcggtt
    29281 ttgtacgcgc gatcgtaagc atcaagaatt gaattgccga tagccacgcc gcaagggttg
    29341 gcgtgcttca caatcacaca tgccggctcg gcgaactctt tcacgcactc cagcgccgca
    29401 tcggtatcgg cgatgttgtt ataagagagg gctttaccct gaacctgggt tgcggtagca
    29461 acggaggctt ctttcacatt ctcttctata tagaaggcag cctgctggtg gctgttctcg
    29521 ccgtaacgca tatcctgctt cttaatgaag ttcaggttca gcgtgcgtgg gaagcgaccg
    29581 gcggcttctt tgctttcacc gtgataagcc ggaaccatgc tgccgaagta gttggcaatc
    29641 atgctgtcgt aggcggcagt gtgttcgaag gctttgatgg cgaggtcgaa acgggttgca
    29701 agcgtcagcg atccttcgtt gtcatccatc tctttaataa tggcgtcgta gtcgctgctc
    29761 ttcaccacga ttgcgacatc tttatggttc ttggcggcgg agcgcaccat cgttgggccg
    29821 ccgatatcga tgttctcaac cgcatcttcc agcgagcaac cttcacgggc cacggtctgg
    29881 gcgaacggat acaggttaac aaccaccata tcgataggct ggatctgatg ttcttccata
    29941 atggcatcgt cctggccgcg acggcccaga atgccaccat gtactttcgg atgcagggtc
    30001 ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat cggaaacttc ggttaccggc
    30061 agaccttttt ctgctaacag acgggcagtg ccccctgtag acagcagctc cacaccgcgt
    30121 gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt cagaaacact gagcagagcg
    30181 cggcggactg gacgacgttg ttgcatggta aatcccctgg atttgactat tacagagagc
    30241 gttagctgaa tttttcgcga aaaactcagc taacgcccct aacggggcat ccttattttt
    30301 cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga atttttttaa ttcgatggcg
    30361 atcacgattt tgcccccgat tagcgtggga gaaatagaga aataacgctg atgattgtgg
    30421 ataactctgt gcgtaaaaag gtataaagcg gggttttgct ggggaatgca gcagtcagtc
    30481 atttttctgc aatttttcta ttgcggcctg cggagaactc cctataatgc gcctccatcg
    30541 acacggcgga tgtgaatcac ttcacacaaa cagccggttc ggttgaagag aaaaat
//
LOCUS       DAZFBR010000053        30337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFBR010000053 DAZFBR010000000
VERSION     DAZFBR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.053"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            222..1088
                     /gene="folD"
                     /locus_tag="ACHJZ2_002577"
     CDS             222..1088
                     /gene="folD"
                     /locus_tag="ACHJZ2_002577"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO4568663.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1090..1302
                     /gene="ybcJ"
                     /locus_tag="ACHJZ2_002578"
     CDS             1090..1302
                     /gene="ybcJ"
                     /locus_tag="ACHJZ2_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO4568664.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1410..1931
                     /gene="ybcI"
                     /locus_tag="ACHJZ2_002579"
     CDS             1410..1931
                     /gene="ybcI"
                     /locus_tag="ACHJZ2_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4568665.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1967..3352)
                     /gene="cysS"
                     /locus_tag="ACHJZ2_002580"
     CDS             complement(1967..3352)
                     /gene="cysS"
                     /locus_tag="ACHJZ2_002580"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO4568666.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3526..4020
                     /gene="ppiB"
                     /locus_tag="ACHJZ2_002581"
     CDS             3526..4020
                     /gene="ppiB"
                     /locus_tag="ACHJZ2_002581"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO4568667.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4023..4745
                     /gene="lpxH"
                     /locus_tag="ACHJZ2_002582"
     CDS             4023..4745
                     /gene="lpxH"
                     /locus_tag="ACHJZ2_002582"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO4568668.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4863..5372
                     /gene="purE"
                     /locus_tag="ACHJZ2_002583"
     CDS             4863..5372
                     /gene="purE"
                     /locus_tag="ACHJZ2_002583"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO4568669.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5369..6436
                     /gene="purK"
                     /locus_tag="ACHJZ2_002584"
     CDS             5369..6436
                     /gene="purK"
                     /locus_tag="ACHJZ2_002584"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO4568670.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(6575..7468)
                     /gene="ybcF"
                     /locus_tag="ACHJZ2_002585"
     CDS             complement(6575..7468)
                     /gene="ybcF"
                     /locus_tag="ACHJZ2_002585"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4568671.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7465..8280)
                     /gene="ylbF"
                     /locus_tag="ACHJZ2_002586"
     CDS             complement(7465..8280)
                     /gene="ylbF"
                     /locus_tag="ACHJZ2_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4568672.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8291..9550)
                     /gene="ylbE"
                     /locus_tag="ACHJZ2_002587"
     CDS             complement(8291..9550)
                     /gene="ylbE"
                     /locus_tag="ACHJZ2_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4568673.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9560..11227)
                     /gene="fdrA"
                     /locus_tag="ACHJZ2_002588"
     CDS             complement(9560..11227)
                     /gene="fdrA"
                     /locus_tag="ACHJZ2_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4568674.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11544..12593
                     /gene="allD"
                     /locus_tag="ACHJZ2_002589"
     CDS             11544..12593
                     /gene="allD"
                     /locus_tag="ACHJZ2_002589"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO4568675.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRQAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12615..13850
                     /gene="allC"
                     /locus_tag="ACHJZ2_002590"
     CDS             12615..13850
                     /gene="allC"
                     /locus_tag="ACHJZ2_002590"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO4568676.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13861..14646
                     /gene="allE"
                     /locus_tag="ACHJZ2_002591"
     CDS             13861..14646
                     /gene="allE"
                     /locus_tag="ACHJZ2_002591"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO4568677.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14874..16019)
                     /gene="glxK"
                     /locus_tag="ACHJZ2_002592"
     CDS             complement(14874..16019)
                     /gene="glxK"
                     /locus_tag="ACHJZ2_002592"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO4568678.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16041..17342)
                     /gene="ybbY"
                     /locus_tag="ACHJZ2_002593"
     CDS             complement(16041..17342)
                     /gene="ybbY"
                     /locus_tag="ACHJZ2_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO4568679.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17399..18760)
                     /gene="allB"
                     /locus_tag="ACHJZ2_002594"
                     /pseudo
     CDS             complement(17399..18760)
                     /gene="allB"
                     /locus_tag="ACHJZ2_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(18797..20188)
                     /gene="ybbW"
                     /locus_tag="ACHJZ2_002595"
     CDS             complement(18797..20188)
                     /gene="ybbW"
                     /locus_tag="ACHJZ2_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO4568680.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
     gene            complement(20357..21235)
                     /gene="glxR"
                     /locus_tag="ACHJZ2_002596"
     CDS             complement(20357..21235)
                     /gene="glxR"
                     /locus_tag="ACHJZ2_002596"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO4568681.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21336..22112)
                     /gene="hyi"
                     /locus_tag="ACHJZ2_002597"
     CDS             complement(21336..22112)
                     /gene="hyi"
                     /locus_tag="ACHJZ2_002597"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO4568682.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKNI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22125..23906)
                     /gene="gcl"
                     /locus_tag="ACHJZ2_002598"
     CDS             complement(22125..23906)
                     /gene="gcl"
                     /locus_tag="ACHJZ2_002598"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO4568683.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(23996..24811)
                     /gene="allR"
                     /locus_tag="ACHJZ2_002599"
     CDS             complement(23996..24811)
                     /gene="allR"
                     /locus_tag="ACHJZ2_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO4568684.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24889..25371)
                     /gene="allA"
                     /locus_tag="ACHJZ2_002600"
     CDS             complement(24889..25371)
                     /gene="allA"
                     /locus_tag="ACHJZ2_002600"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO4568685.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25601..26527
                     /gene="allS"
                     /locus_tag="ACHJZ2_002601"
     CDS             25601..26527
                     /gene="allS"
                     /locus_tag="ACHJZ2_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO4568686.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26596..27690
                     /gene="mnmH"
                     /locus_tag="ACHJZ2_002602"
     CDS             26596..27690
                     /gene="mnmH"
                     /locus_tag="ACHJZ2_002602"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO4568687.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            27806..28171
                     /gene="ylbG"
                     /locus_tag="ACHJZ2_002603"
                     /pseudo
     CDS             27806..28171
                     /gene="ylbG"
                     /locus_tag="ACHJZ2_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28921..29181)
                     /locus_tag="ACHJZ2_002604"
     CDS             complement(28921..29181)
                     /locus_tag="ACHJZ2_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568688.1"
                     /translation="MLAISSNLSKMIIFIIAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFPHVAVDISIYTNVEDDYFFLIFP"
     gene            complement(29162..>30337)
                     /locus_tag="ACHJZ2_002605"
     CDS             complement(29162..>30337)
                     /locus_tag="ACHJZ2_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4568689.1"
                     /translation="RRTGKRVWRRGRDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPLALI
                     SEDGNTAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGR
                     YITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTY
                     EDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRK
                     VKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKETIKALQDAGYLK"
BASE COUNT         7459 a         8253 c         7458 g         7167 t
ORIGIN      
        1 ttaatcctta aacaaggaaa taaatcagtt ccagatttac aacgccatca tggacgaaaa
       61 atgaagcttt caatctcagc gacctcagcg acagtgcgcc taaccttcgc aagaggtcgc
      121 ttcacgcgat aaatctgaaa cgaaacctga cagcgcgccc cgcttctgac aaaataggcg
      181 catccccttc gacctacgta acagatggaa tcctctctct gatggcagca aagattattg
      241 acggtaaaac gattgcgcag caggtgcgct ctgaagttgc tcaaaaagtt caggcgcgta
      301 ttgcagccgg actgcgggca ccaggactgg ccgttgtgct ggtgggtagt aaccctgcat
      361 cgcaaattta tgtcgcaagc aaacgtaagg cttgtgaaga agtcgggttc gtctcccgct
      421 cttatgacct cccggaaacc accagcgaag cggagctgct ggagcttatc gatgcgctga
      481 atgccgacaa caccatcgat ggcattctgg ttcaactgcc gttaccggcg ggtattgata
      541 acgtcaaagt gctggaacgt atacacccgg acaaagacgt agacggtttc catccttata
      601 acgtcggtcg tctgtgccag cgcgcgccgc gtctgcgtcc ctgcaccccg cgcggtattg
      661 tcacgctgct tgagcgttac aacattgata ccttcggcct caacgccgtg gtgattggcg
      721 catcgaatat cgttggccgc ccgatgagca tggaactgtt gctcgcaggt tgcaccacca
      781 ccgtgactca ccgtttcact aaaaatctgc gtcaccacgt agaaaatgcc gatctgttga
      841 tcgttgccgt cggcaagccg ggctttattc ctggtgactg gatcaaagaa ggcgcaattg
      901 tgattgatgt cggcatcaac cgtctggaaa atggcaaagt tgtgggcgac gtcgtgtttg
      961 aagacgcggc taaacgcgcc tcatacatta cgcctgttcc gggcggcgtt ggcccgatga
     1021 cggttgccac gctgattgaa aacacgctac aggcgtgcgt tgaatatcat gatccacagg
     1081 gtgagtaaca tggcgacatt ttctttaggt aaacatccgc acgttgagct gtgcgacttg
     1141 ctgaaactgg aaggctggag cgaaagcggc gcgcaggcga aaatcgcgat tgccgaaggc
     1201 caggtgaaag tcgacggcgc ggttgaaacg cgcaaacgct gcaaaatcgt cgccgggcag
     1261 acagtgagtt ttgcaggtca cagcgtacag gttgttgcct gatccctttc ccggcctgta
     1321 tcaacaggcc ggttctcttt tcagactttt gccctctacg ccccgttcat ccatgctgat
     1381 agcatcgaca ttttgccgaa gcgaccatca tgccaaccgt tattacgcac gccgccgttc
     1441 ccctatgcat tggtttaggg ttgggttcga aagtcatccc tccgcgcctg ttatttgccg
     1501 gaatcatcct ggcgatgctg cccgatgccg acgtcttgtc gtttaatttt ggcgttgctt
     1561 acggcaatgt ttttggtcat cgcgggttta cccattcgct ggtgtttgcg tttgttgtcc
     1621 cgctgttatg tgtgctgatt ggacgtcgat ggttcagggc tgggctgatt cgctgctggc
     1681 tgtttttaac cgtctcgttg ctatcgcaca gcttgctgga ttcggtaacc actggcggta
     1741 aaggggttgg ctggctgtgg ccgtggtcag atgaacgctt tttcgctccc tggcaggtga
     1801 ttaaagtcgc gccgtttgca ttgtctcgtt acaccacagc gtacgggcat caggtgatta
     1861 tttctgaatt gatgtgggtg tggttgccgg ggatgctact gatgggaatg ttgtggtggc
     1921 gcagacgata accggatgcg aaaactcgca tccggcaata gcgcaattac ttacgacgcc
     1981 aggtggtccc ttgcgggcca tcttccagca cgatccccat ctcattaaga cggtcacgcg
     2041 ccgcatctgc cgccgcccag tctttcgctt tacgggcatc cagacgctgt tgaattaacg
     2101 cttcaatctc agccacttcg ctgtcgtctg cctgcgcgcc gctttgcagg aacgcttccg
     2161 gttcttgctc cagcaggccc aatacggcgg aaagtttacg caggtgagat gccattgcat
     2221 tcgccgctgc catatcttct actttcagac ggtttacttc acgcgccata tcaaacagca
     2281 cggaataggc ttccggggtg ttgaaatcgt cgtccatcgc ttcaataaag cgcgcttcaa
     2341 acgcttcgcc accggcaggc gcaacggttt tatctgtgcc gcgcagcgca gtgtagagac
     2401 gctccagcgc cgcacgcgcc tgcttcaggt tctcttcgct atagttcagc tggctgcgat
     2461 agtggcccga catcaggaag taacgcacgg tttccgcgtc gtagtatttc agcacatcgc
     2521 gcacggtaaa gaagttaccc agcgatttgg acatcttctc gcggtcaacc atcaccatcc
     2581 ccgagtgcat ccagtagttc acatactgac catcatgggc acaggtggac tgcgcgattt
     2641 cgttttcgtg gtgcgggaac atcaggtctg aaccgccgcc gtggatatca aagtggttac
     2701 ccagctgctt gcagttcatt gccgaacatt caatgtgcca gccaggacga cccgcgcccc
     2761 acggagacgg ccagctcggt tcgccctctt tcgacatctt ccacagaacg aagtccattg
     2821 ggttgcgttt gtcgtcgacc acgtcaacgc gcgcgcctgc ctgcagctgg tcgagatcct
     2881 gacgcgacag cacgccataa gttggatcgg tcgggacgtc gaacatcacg tcgccgttgt
     2941 ccgccacata agcgtgacct ttggcgatca gttgttcagt gagttcaata atttctgcga
     3001 tatggtgcgt cgcgcgcggc tccatatccg ggcgcagaat gttcaacgca tcaaaatctt
     3061 tgtgcatttc ggcgatcatg cgatccacca gcgccacaaa gctttcgcca ttttcattgg
     3121 cgcgtttgat gattttgtcg tcgatatcgg taatgttgcg cacatacttc agcttatagc
     3181 cgaggaaacg cagatagcgc gcaaccacgt caaaagcaac aaaggtacgc ccgtgaccga
     3241 tatgacagag atcgtaaacg gtgattccac acacgtacat gccgacttcc ccggcgtgaa
     3301 taggcttaaa ttcctctttt tggcgtgtca gagtattgaa gatttttagc atcgaagatt
     3361 ccgtttagac atgtgtgggt aattgagttg cgtataatac ccatatttcc cgcgcgaatc
     3421 agcatacatt gcgtgatgat ccgatcgtgc ggttatgcta taacaccacc ctatatatga
     3481 cccgaactgg gttgaagcac caatcaaacg gaacaggatg caaaaatggt tactttccac
     3541 accaatcacg gcgatattgt catcaaaact tttgacgata aagcacctga aacagttaaa
     3601 aacttcctgg actactgccg cgaaggtttt tacaacaaca ccattttcca ccgtgttatc
     3661 aacggcttta tgattcaggg cggcggtttt gaaccgggca tgaaacaaaa agccaccaaa
     3721 gaaccgatca aaaacgaagc caacaacggc ctgaaaaata cccgtggtac gctggcaatg
     3781 gcccgtactc aggctccgca ctctgcaact gcacagttct tcatcaacgt ggttgataac
     3841 gacttcctga acttctctgg cgaaagcctg caaggttggg gctactgcgt gtttgctgaa
     3901 gtggttgaag gcatggacgt ggtagacaaa atcaaaggtg ttgcaaccgg tcgtagcggt
     3961 atgcaccagg acgtgccaaa agaagacgtt atcattgaaa gcgtgaccgt tagcgagtaa
     4021 tcgtggcgac actctttatt gcagatcttc atctctgcgt ggaagaaccg gcgatcaccg
     4081 ccggttttct gcgtttttta gcgggagaag cccgcaaggc cgacgcgctg tatattcttg
     4141 gcgatctgtt tgaagcatgg attggcgacg acgatcccaa cccactccat cgccagatgg
     4201 cggcagcgat caaagcggtg tccgattccg gcgttccctg ttatttcatt catggcaacc
     4261 gtgattttct gctcggcaaa cgctttgccc gtgaaagtgg catgacgtta ttgccggaag
     4321 aaaaagtgct cgaactttat ggtcgtcggg tgctgattat gcatggcgac acgctgtgca
     4381 ccgatgacgc gggttatcag gcttttcgca ccaaagtcca caaaccctgg ctgcaaacgc
     4441 tattcctcgc tctgccgttg tttgtgcgca aacgcattgc cgtgcgaatg cgcgcgaaca
     4501 gtaaagaagc caacagcagt aaatcgctgg cgatcatgga cgttaaccaa aacgcggtag
     4561 tcagtgcgat ggaaaaacat caggtgcaat ggctgatcca cgggcatacc catcgcccgg
     4621 cggtgcatga acttatcgcc aatcagcaac ctgcttttcg cgtggtactg ggtgcctggc
     4681 atacggaagg ttcgatggtg aaagtcacgg cggatgacgt tgagctgatt cattttccgt
     4741 tttaaaaaac ccgcaacttt gctgatttca cagccacgca accgttttcc ttgctctctt
     4801 tccgtgctat tctctgtgcc ctctaaagcc gagagttgtg caccacagga gttttaagac
     4861 gcatgtcttc ccgcaataat ccggcgcgtg tcgccatcgt gatggggtcc aaaagcgact
     4921 gggctaccat gcagttcgcc gccgaaatct tcgaaatcct gaatgtcccg caccacgttg
     4981 aagtggtttc tgctcaccgc acccccgata aactattcag cttcgccgaa agtgccgaag
     5041 agaacggtta tcaggtgatt attgcgggcg caggcggcgc agcgcacctg ccaggcatga
     5101 ttgccgccaa aacgctggtg ccagtgctgg gcgtgccagt acagagcgcc gcactgagcg
     5161 gtgtcgatag cctctactcc atcgtacaaa tgccacgcgg cattccggtg ggtacgctgg
     5221 cgattggtaa agctggcgcg gcaaacgcgg cattactggc agcacaaatt cttgcgactc
     5281 atgataaaga actgcaccag cgtctgaatg actggcgcaa agcacaaact gatgaagtgc
     5341 tggaaaaccc ggacccgcga ggtgcggcat gaaacaggtt tgcgtcctcg gtaacgggca
     5401 gttaggccgt atgctgcgtc aggcaggcga accgttaggc attgctgtct ggccggtcgg
     5461 gctggacgct gaaccggcgg cggtgccttt tcaacaaagc gtgattaccg ctgagatcga
     5521 acgctggccg gaaacagcat taacccgcga gctggcgcgc catccggcct ttgtgaaccg
     5581 cgatgtgttc ccgattattg ctgaccgtct gactcagaag cagcttttcg ataagctcca
     5641 cctgccgact gcaccgtggc agttacttgc cgaacgcagc gagtggcctg cggtgtttga
     5701 gcgtttaggt gagctggcga ttgttaagcg tcgcactggt ggctatgacg gacgcggtca
     5761 atggcgttta cgcgccgatg aaaccgagca gttaccggct gaatgctacg gcgaatgtat
     5821 tgtcgagcag ggcataaact tctctggtga agtgtcgctg gttggcgcgc gctgctttga
     5881 tggcagcacc gtgttttatc cgctgacgca taacctgcat caggacggta ttttgcgcac
     5941 cagcgtcgct tttccgcagg ccaatgcgca gcagcaagcg caagccgaag agatgctgtc
     6001 ggcgattatg caggagctgg gctatgtggg cgtgatggcg atggagtgtt ttgtcacccc
     6061 gcaaggtctg ctgatcaacg aactggcacc gcgtgtgcat aacagcggtc actggacaca
     6121 aaacggtgcc agcatcagcc agtttgagct acatctgcgg gcgattaccg atctgccgtt
     6181 accgcaacca gtggtgaata atccgtcggt gatgatcaat ctgattggta gcgatgtgaa
     6241 ttatgactgg ctgaaactgc cgctggtgca tctgcactgg tacgacaaag aagtccgtcc
     6301 gggccgtaaa gtggggcatc tgaatttgac cgacagcgac acatcgcgtc tgtccgcgac
     6361 gctggaagcc ttgatcccgc tgctgccgcc ggaatatgcc agcggcgtga tgtgggcgca
     6421 gagtaagttc tgttaacctg gtgctcattt gattgccgga tgcggcgtga acgccttatc
     6481 cggcctacac cgatccggta cccattgtag gcctgataag acgcgtcaag cgtcgcatca
     6541 ggcatggtgc gccgattgcc ggatgctctc atcactacag cgaaatacag gtccccgctt
     6601 cgcccgccag cgtctcttca attcgcgata acgccccaat ccacgcgggt ttaccacggc
     6661 ttctgacata accactcacc gccgttacct tcggccccat cgaaccatcg gctttggcaa
     6721 atggcgctaa ctcatccggt gtggcatggc gaatggcacg ttgctgcggc attccccagt
     6781 tttcatatac cgcatcagca tcggtgagga tcaccagtcc atcggcgtta atttgctcgg
     6841 cgagcaacgc agcggcgaga tctttatcaa tcactgcttc actccctgct ccgtcatccg
     6901 tcacaggcac accgccgccg ccactgcaaa tcaccacatg cccctctttg agcaacaact
     6961 cgatggcttc gctgtcgaga attttacgcg gttgcggaga cgccaccacc cggcgcaaat
     7021 atttaccatc acgtttcatc tgccagccgt aagccgcttc cagtgcctct tgttcttctg
     7081 gctgataaac cggaccaata aatttctctg gctgcaaaaa cgccggatca tcaggcgaaa
     7141 cttcaatgcg cgtccgcacc gtcgtgacag gcggcatctg cggctgtgcg ctcaaactct
     7201 gcgccagcat atagccaatc atcccctgac tttctgccac cagcacatcc agcggatacg
     7261 gttctacctc tttccacgcc agattctgca atgccagcag ccccacctgc ggcccgttgc
     7321 cgtgaacaat cgccagccga taagaacggg ccaggcgcgc cagcgcgggt acagcactgg
     7381 cgatattgcg atattgattt tctgccgtca gcgcctcacc gcgctggagt aaggcgttgc
     7441 ccccaagagc tacaaccagt gttttcatgg ttttccttgt aataattgtt ggccaagcca
     7501 gaaccccagc agtgtatctg cccctgacgt atgccccagc gccagcagcg aatcaatcgc
     7561 aacagcggta cgtttcgggc aactcagagc atgaacaaag tgcaggagtg gcgaggcgaa
     7621 atatccttgc gcggcataac gtaaataact gacgctcacc gctgtggtaa cgagttgcag
     7681 attgtcggaa caggcaaaaa acggacgacc ggagcgcgca tctaaagcac cataatacca
     7741 ggccgccagc agcattccgc tcagcgtgtc atcatgactt ggcgttaatc cggggccttt
     7801 acccagccag tgccgccagt cggtcttaac gccattgagc gcggcctgaa aacagtgacg
     7861 aaactggcgt aactcagcag gcagcggatc gcttgccgcc aacgccagtg gcccgaaaag
     7921 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagt ggttgaggat gcgcaggcgg
     7981 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     8041 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     8101 ttgcgcacgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     8161 taacagttcg ccgctttccg tcatcaggtt aatcgcccgc cgccacactc ccgctaaccg
     8221 ccaggactgg cgataattcg gtgcgctact actggcaagc agaggatgga tgatcgtcat
     8281 tacgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaagc
     8341 acgccagcgg tgcccgcacg gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8401 cgataccggt attgatgagt ggcgtaatgc cggtttctac cacgcgacga atgtccagtc
     8461 ccaggcacgc gccctgaaaa tcccagcttg ggatctgcaa ctgcatattg cgttcaaggt
     8521 aaatttccgc catctcttca gataccgccc tggccgcttc catgccactc gcaccgacaa
     8581 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8641 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8701 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8761 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8821 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8881 cgctgaggaa atccatcact tccgcgatgt gctgtttatc atgatcgagg cgagcaattt
     8941 gtggggccag cgcacgcatt aacagtgctg aggaagcaat attgcgttga tggaactcat
     9001 cgcccatcgt aatgccctgc gccatcatcg cagtaagatc gataccgcgc tccatgcgcc
     9061 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     9121 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9181 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9241 taccgcccat tggcccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9301 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca accttcgaac agacatgcgc
     9361 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9421 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9481 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9541 atgtaaacat gagaacccct tattgcaaac gttctaataa acgagccagt tttttattgc
     9601 cgccggcgac tggcgaccat tggtaatgca caaccggttt gctggcgctt tgtagctcca
     9661 gcgcaaaact gcgtaatccg atgttaatca cggcgacgtt ttccagtaat gacggtgtgt
     9721 gttgctgtgc ggcaggcgag agcggatgaa ttaacgctgc cgccagcaag gtggcttccg
     9781 gtagcgaact cacgaccgca atccccgcat cttccagcgt ggcgatttgc tgcgagcggc
     9841 attgcgggtc acgttcagtg cctgtcaccg tggcaatggc atacagtggt tgattgtctg
     9901 aacgcgcagc acaggctttt tgccaggcgc ttaccagcga ggcggcagga tcggcggtcg
     9961 caccgaagcc aatcacgaca tcaagcagca gcacacgcac ttgcggttta gcgccgagat
    10021 cggtaattaa ctggttgcgt aaggttgggt cgatcatcgg atggggacgc ccgacggtgt
    10081 agaaatcatc gccgaggtca ataatctggt ggccatcggc gtccagcatc atgccgtgat
    10141 gatgggcatc atcggcttcc acgccaaggt gtccggcaag taatcccgcc gcttcggcag
    10201 ccagcgtacc gccggtatac aaaccgcaaa taaatccgct gctggcaggc attatcgcgt
    10261 tacgtcgcgc cgtaacgcgt gaaagcaggc aagccaggcg tgcggcctca tccagcgagg
    10321 aggcaaacca gacattctcg tcgcgggcca ccgccggggt ataacctaaa aacagcgcca
    10381 ccgtcggttt gccggttgct ttcatggcat taacaatttt cagacgcacc gcttcggcag
    10441 gtggttttga aacaaatgcc agcacttcgc ttttctcgtc tgcactgagc atttccagcg
    10501 ccgtcagcgc actgatgccg cccacttcac ggctgaggtc gcggccgcca aggccaatcg
    10561 cgtgagtaat tccctcccct gccagcgcaa tctgcgagca cagctcctga atcccggtac
    10621 cggaagcgcc aatgacgcca atgttgcctt ccggcatcac gttagcaaaa gccagcggcg
    10681 tgccggcaat catcgacgta ccgcagtccg gccccatcac cagcaagcct ttttcccgcg
    10741 cgcgggtttt aagttggatt tcatcttcca gcgtgacgtt atcggagaac atcatcacgt
    10801 tgaggttgcg atccagcgcc tgattcgcca gctccgccgc atactcgcca gccactgaaa
    10861 tcagcgccag attggcatcg ggtaatttct gacaggcact gtcccagcga cgcacctgcg
    10921 tcaacgcctg gctgctgcct gacccctgcg ccagttgttt tagcgcctct tcaagctgct
    10981 gcataatcgc ctgcgcgatc cccgcatccg ccgcttcgct acgaattgcc acgcaaatat
    11041 cgttcggcgt ggcgttatta aaatcgtcat gccagaaacc tgtggtatct aataacgctt
    11101 tattggcggg cgtacccatc attacggaaa catcatcaac attttctgat tcgctgagtt
    11161 ttcgtgaaat aatcattaaa ctgaccgaat cctgaaaaca ccctttttta ataaaggcgt
    11221 ggatcataac aactccttaa aaattgcacc catggcaatt gcacgagcag tgttaatgtg
    11281 gcgattacgg tatcgatgac aacgagtaaa taaggtgatg aatatcacac agggattatt
    11341 aacaaaagaa ttcaaataac cgccatgcta ttacgtgtta ttttttaata agcgtggttg
    11401 ttttaaaaac caggttaaaa gcgagcggat tatcaaataa attctaacta tatttttttg
    11461 cctgtctgga tcacataatc cagatatttt ccctgtatgt taattgcagt catgcttcac
    11521 accgtcgtta aaaaggaaga cagatgaaaa tcagtcggga aacactccac cagctaattg
    11581 agaataaact ctgccaggct gggttaaaac gtgagcacgc tgcaaccgtg gctgaagtat
    11641 tggtttatgc cgatgccaga gggatccact ctcatggcgc ggtgcgcgtg gaatactacg
    11701 cggaacgcat ttcaaaaggc ggcaccaacc gcgaaccgga gtttcgtctt gaggaaaccg
    11761 ggccgtgctc ggcaatttta catgccgaca atgccgccgg acaggtcgcg gcgaaaatgg
    11821 gtatggaaca tgccatcaaa accgcccagc aaaatggcgt tgcggtggtc ggtatcagcc
    11881 ggatgggtca cagcggcgca atctcttatt ttgtgcagca ggcagcccgc gccggattaa
    11941 ttggcatttc gatgtgccag tccgatccga tggtggtgcc gtttggcggc gcggaaattt
    12001 actacggtac taacccactg gcctttgccg cgccgggaga aggcgacgag atccttacct
    12061 ttgatatggc gactaccgta caggcatggg gaaaagtcct cgacgcccgc tcgcgtaata
    12121 tgtctatccc ggatacctgg gcggtcgata aaaacggtgc accaacaacc gatccgttcg
    12181 cggtacatgc tctgctcccc gcagccgggc cgaaagggta tggcctgatg atgatgattg
    12241 acgtcctctc aggcgtctta ctcggcttac cgttcgggcg acaggttagt tcgatgtatg
    12301 acgatttaca cgccgggcgt aatttggggc aattacatgt agttattaac ccgaactttt
    12361 tctcctccag cgaattattc cgtcaacatc ttagccagac catgcgcgaa ttaaatgcca
    12421 ttacccccgc gcccggtttt aatcaggttt attatcccgg acaggatcag gatattaaac
    12481 aacgccaagc cgccgtcgaa ggcatcgaaa ttgttgatga tatttaccag tatttaattt
    12541 ccgacgcgct ttataacacg tcatacgaaa cgaaaaatcc ctttgcgcaa taattattga
    12601 gacaggactt tcttatgatt acacattttc gtcaaaccat agaagaagcg ctgccctggc
    12661 tttcctcttt tggcgctgac ccagcgggtg ggatgacccg tttactttat tcgccggaat
    12721 ggctggaaac ccagcagcaa ttaaaaaaaa gaatggccgc aagtgggctg gaaacacgtt
    12781 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtga
    12841 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    12901 gcgcgctggc ggcgcggctg gcaattgact ggctgaaaac tcaatacggc gcaccgctgc
    12961 gtacggttga agtggtggcg atggcggaag aagaaggtag ccgcttcccg tatgttttct
    13021 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    13081 ccaaaggaaa tagttttgtc gatgcgatga aggcttgcgg atttactctg ccggacgccc
    13141 cgctaactcc gcgtcaggat attaaagcct ttgtcgaact gcatatcgaa cagggctgtg
    13201 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg caacgtcgct
    13261 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    13321 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    13381 tgggcgatcc gctggttctg acctttggga aagtagagcc gcgcccgaat acggtgaatg
    13441 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgcc gccgtgctgc
    13501 gtgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattc
    13561 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gacctggtcg
    13621 ccaccctgac agaattgtgt gaaagtgaaa aactgaatta ccgggtgatg cacagtggtg
    13681 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    13741 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattaccgac cttgccgaag
    13801 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcagaaataa ggagtcataa
    13861 atgggatatt taaataacgt caccggttac cgcgatgatt tactggctaa ccgtgcgatt
    13921 gtgaaacacg gtaatttcgc actgttaaca ccagacggtc tggtaaaaaa tattattccg
    13981 ggctttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    14041 gattatctgg tcacactgca tcaaaacggc ggcaaccaac agggcttcgg tggcgaaggc
    14101 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    14161 ttttccttaa gcgaaggtgg ctatctttat tgcccgcctg gctccttaat gacgtttgtt
    14221 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    14281 ggctatgcac cgtggctggt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    14341 ggtatggatg atgtgatcct gctggatttc ctgcccaaag agttaggttt tgatatgaac
    14401 atgcatatcc tttcttttgc accaggtgcc agccacggtt atatcgaaac acatgttcag
    14461 gaacacggtg cctatattct ttccggtcag ggggtttata acctcgacaa taactggatc
    14521 ccggtgaaaa aaggcgatta catctttatg ggcgcttatt ctttacaggc tggttatggc
    14581 gtagggcgtg gtgaagcgtt cagctatatt tactcgaaag attgtaaccg cgacgtagag
    14641 atttaagtca taccactcgc ctgattattt ttagctatgt tggattttgc cacggtttat
    14701 accgtggctt tttttgcaac atagcctgat gcgcttcgct tatcaggcct acatgatctc
    14761 tgcaatatat agaatttgcg cgattttcag gaaggatatg gtactcatgc cgcatccggc
    14821 atgagtactg cactcttatg cgatataaat cgcatccgct ttaaaggtaa gggtcagttt
    14881 ttaattccct gacctatttt aatggcgcag gcaatatttc gcgcgctgtt gaagagattg
    14941 gtttcaccgc tggcgagcac ttcggctaaa ggtgccagac gtggcaaaat gctgaatacc
    15001 gcatcaatgc cgtactggtg caccacttcc acgccatcac ccaatacgcc agcaatccca
    15061 atcaccggta cattaaactg cttcgccacc gacgcaacgc ccagcggcgc tttaccgcct
    15121 gccgtttgcg agtcaatgcg cccttccccg gtgatcacca gtgctgcccc ctgcaccgcc
    15181 tgctccaggt tgaccgcccg cagtacaatc tcaatacccg gtttaatatc cgcattgagg
    15241 aataccgccg ccgcaatccc cataccgccc gcagcgccac cgcccgccat ctggcagaca
    15301 ttaatttcag tttgctgttg taaaacacgg gcgtaatttt gcagcccctg ttcaagttct
    15361 tcgaccatct ccggcgttgc ccctttttgc gggccaaaca ccgccgccgc gccgcgtgcc
    15421 cctaccagcg ggttatcaac gtcacatgct acttcaatat ggcagttcgc cagacgcgga
    15481 tcgcattcct ccatctcaat gcttgccacg cgcgctaaat tgccgccgtt agccaccagc
    15541 acctgaccgt cggcatcaag gaaacgcaca ccgagcgcct gcgccatgcc cataccgccg
    15601 tcgaccgtcg cactgccgcc aatgccgaga ataatatggc gaatgccgtt atccagcgca
    15661 tgacgaatta actcccccgt accaaaactg gaggccagca acggattacg cttttcaggc
    15721 gcgaccagca tcaggccact tgctgccgcc atctcaatca ccgccgtttt cccgtcgccg
    15781 gtaatgccat aaaaagcatt cactttttcg cccatcggcc cgcagacttc aagcgtcacg
    15841 atgttgccgc ccgtcgcggc gaccatcgca tccaccgtcc cttcgccgcc atccgctatc
    15901 ggcaaacaga tatagttcgc atcgggaaag agggtcgaaa acccggcttt aattgcctga
    15961 caacattttt ctgcactcaa gctctcttta aaagagtctg gcgcaatgac aatcttcatt
    16021 atcaatcctt tttcaacaaa ttattcgatg cgttcccacg gaataaggtt atccataaga
    16081 acagccagta aaatcccgac caataagccg ttactgagca gaggacgaag cgttaatgga
    16141 aggtcttgca gatacacagg cggtaatgcc attaaaaata tgccgacaaa taacggcaat
    16201 gcgagtcgat aaatattgcg agcggtaaac gttatttgct ggctaaacac cagcgcggaa
    16261 aagagtaaag gtagataaga aaccagcatg accgcactac tcacgggtaa agggatactg
    16321 caaaacagtc gcgtgagtgc cggaaccagc gccaccagca ggcaaataac gctgccataa
    16381 ataaacgaac gccgcgtgta atcgccagtc tgggttaata aaccaatgga tgaaacaaac
    16441 ggtgaaaatg gaattactgc cagcggcacg gttatcagtg tcataaatcc ggtcgccaca
    16501 aagctacgac gataacgcgt attccccgcg ccctgctgcg gataaaaaac atccgtgccc
    16561 cgaatcgcac cgtaggtatt gctgatattc accagacctg taatcaccgc cgtcagaata
    16621 atccccggcg acaaagcacc gccactgccg agcgggaacc actgccagtg caactcaccg
    16681 gagagcgaat gcgaagaagg aaagcaaaag taccacaaca accagccggt tatggtgccg
    16741 accagcaggc cataacgggc aaaacgttgc ggcagaaaga taatcatcgc cagtaccagg
    16801 cacatcaccg ccaccgagag cgcgaacggc ggtaactgaa ttttaaaatt cgggtcggct
    16861 atgccaaacg gcagcccgag catacctttg aaaaagatag tggtcagctg cgcgcccagc
    16921 atcaacataa acaagaccat caccgacggc gtaaataacc gcgccaggcg atgacctaat
    16981 ccgctaaaac caatcaacat cgtcagcacg ccggagagtg caatccccac cgccaggctg
    17041 gtagcgatat cgttgatcgg tgtcccgcgc gatgcttcgc caagggtgat agttaggatg
    17101 gttccccacc acaggccccc cggcccttcc ataatagcgc gacgatgacc gcaaaacgcc
    17161 tgagcgaagc aggccagtgc ggtagcaaga aaagcatatt gcgtgagcgt aagcaggcta
    17221 ctttgcggca actgaaaagc agaaagtagc gtaggaggaa ccacaaccgt gttgcaaaaa
    17281 ataaagaaaa accactgaaa tcctgataac aggctttcgc ggctaactgc aaaattgaac
    17341 ataaacacac cttaaccgga gaatgcccgc cgcaaggacg ggcattgcag gggccagatt
    17401 actgctgatg tttaaggata aattgacctt tcggcgcaac agggaagccc tgttcgatgt
    17461 cgtaaatcac atcaccacgt aagatggttt tcgtgatacg cgcgccaatg gtacggccaa
    17521 catacgggct gactttgtgg cgatattcca gatcgtcatt ggtaagaaca tagctgctat
    17581 tcggctgaat gaagacgaag tcggcatctt ttcctggggc gatacggcct ttttgctgca
    17641 gaccaaaaat atctgctgcg ttagtcgcca ttaatttgcc gaacattggc agagacattc
    17701 cgcgtttctg taccgcttca tcgaacatca cgtccataca gttttgcaga ccggcaatac
    17761 cgccccatgc ttccatgatg ttgccggctt tcatttccgg agggcatggt gagtggtcgg
    17821 aaaccaggcg gtcgatttca ccgttaaaca gtttttccca catgcctttc tagttttcca
    17881 gatcgcggat cggcggtgaa cacttcgcca gagtaccgat ttcttcgaac tgatcggtat
    17941 ccagtacgaa gtaatgcggg caggattcac aagtaacgtc ctgaccttcc tgacgtgcac
    18001 gagtcacttc ttcaacgcct tccgggctgc tgatatggca aacgtgcaga cggcaaccgg
    18061 caactttcgc cagatacagc acgcggcgaa tcgcttccac ttcggtaaat accggacgcg
    18121 aagccacata gtcatgggca gttacgcgac cttcacgttt cgcttcttcg cccagtgcgt
    18181 cacagatcag cgcgttttcg cagtgcacca gcaccggctg ccccagttcg cccagcttct
    18241 gcgcaccttt gaagaactgc cagtcgttta catcacggaa gtcgttgtcg ataccgcgat
    18301 cgccacaggt cgcaacgaag catttgaagc cgacaacgcc cacttcatcc agctcatgca
    18361 gacggtcgat gttgtaagac accaggccac cgagttgtgc cgcatcgata gtcagcttgc
    18421 ctttagcggc atcgaacttc agttcaattg aagcgcggtc aaccgttgca ggcagctggt
    18481 tgagcggcat ttcgatcatg gtggtgatac caccttttgc cgctgcgcga gtaccggttt
    18541 cataaccttc ccagtggcta cgacccggtt cagaaatatg ggtgtgcgca tcaaccatgc
    18601 ccggcgaaac caccagacca gacgcatcca taacttcttt tgcatcgccc agatcctgac
    18661 cgatagcagc aattttgccg cctttaacgg cgatatctac aacgcgagct tcgttttcta
    18721 aaataacggt gccgttttta atgattaaat caaaagacat aacaaactcc ttgtaaaata
    18781 cagattggtt tttaactcaa gcaaaggcga cgatgacgcc gacaaaccat gaaacacgcg
    18841 ataacggttc cattaaggga ataaacttac cgccaagaga taaaataacg gccaccagag
    18901 ttactgaaaa cgcggtgagg ttaaaaccgt tatcgtaata tttataatcg ccaggtgcgg
    18961 tgtacagttc atcaagatta atttgcccac gcatcaccac aaaataatgc gccatcatga
    19021 caccaattac cggaccaagc attccgccga taatatcgag gaacagataa atactgtcct
    19081 gattttccat taatttccac gggcagatca gcaggctgat aatactggca atcagtacgc
    19141 cgtttttata ggtcagtttt gtcggtgcaa tggcagcaat ctgataacca gccggaataa
    19201 tattaccagt ggcgttagtg gagatagtcg tcatcagaat aaccagtacc gcaaagaacg
    19261 aggcaaacag gctgtcccaa cgctgaacaa tatccagcac gttccaggta tcagcgccgt
    19321 aatgaatact ggctccggca ataatacata cccccgcgac cgcaaacaga atataggcca
    19381 caactaaacc cagcgtttgc cccagcgctt gctcacgaaa cgagtgggcg ttttgcgtaa
    19441 agtcggatgc gctcaccgcc ggtgccgccc agaccgcaac taccgcgtta atcaccacca
    19501 ggaacaagaa tccactgttt tctgctttct gaataccgct cggaatgtag tcaaagattg
    19561 gaccgagccc gaccagcgaa atcgcccaaa tcgccatacc gccgaaaacg atatagatgc
    19621 acgggttaag aatggcagtg aatttattta aaactttgcc accgccaaaa cctataccaa
    19681 cgttgaccag ccagaagagt aagaaagtaa ttaagcccgg tagagaaagg cctaacagag
    19741 tgaaatcacc accgagagtc aaaaatcccg gccagatttt gccaatcaga atcaagcagg
    19801 ccagtgaccc cgcgtaacat tgcaggccaa accacataat ggcagcaata ccgcctctta
    19861 ataatccggg aaacagtgcg ccacgtacgc cgtaagaagc acgcaggatc atggcaaacg
    19921 gtacgccgta tttactgccc gcagcgccgt ttaataccat taccgcggca atgaaaaagg
    19981 cgctgaggat aattgccagc ataatactaa aggtagacaa gccgagaata aaaaagccgc
    20041 cgaccatcac ataattggga acgttatgaa ccgaacccat ccataaggta aaatagttaa
    20101 atgttttcca ggtccgctgg ctttgcgttt tcggcaatag atcttcgcta tagccgcgtt
    20161 gctggaatag ttttctctga tgttccataa atccctctta atttcacgta tcgaataacg
    20221 ttgttttaat ctagctatga ggaaaaaata aaataaaaat attattttta atactgaagc
    20281 aacatctttg gctgctttct tttttaatat tgctggccga ttcatttttt gatatcggcc
    20341 atttttattg cgggtatcag gccagtttat ggttagccat taattccagc gcctgcacta
    20401 acgcagagtg atccaactgg ctgccaccgt ttgccgcgca ggtattaaat aactcctggc
    20461 aggtcgcagt gtttggcagg ttcagagcca gtgccttcgc actttgcagt gccaggttga
    20521 gatctttctg gtgcagcgca attttgaaac ctggattaaa ggtgcgttta atcatgcgct
    20581 caccgtgaac ttccagaata cgtgaggaag caaagccgcc catcagcgcc tggcgcacac
    20641 gtaccgggtc tgcaccggct tttgaagcaa acagcagagc ttcagagacc gcttcaatat
    20701 tgagcgctac gataatctga tttgccactt tgcaggtttg accgtcgccg ttaccgccca
    20761 caagggtgat atttttaccg agcagttcaa acagcggttt aacacgttca aataccgctt
    20821 cgtcaccgcc aaccataatt gacaacgtcc cttcacgcgc gccgatttcg ccgccggaga
    20881 ctggcgcatc gagataatca ccacccagtt cattcacctg acgagcgaaa cgtttagttt
    20941 caatcggtga aatggagctc atatcaacaa tggttttgcc cttcagcgag gctttggtac
    21001 aaccattttc accaaacaga acttcttcaa cctgaggtgt gtccggcacc ataataaaaa
    21061 tgatgtccgc tgcttccgtt acctggcgcg cggtgtcgac attaacagca ccgagtgaca
    21121 gtaattcatc agcaaccggt ccaatggtcg tgacatgtaa ttgatgaccg gcacgcgcca
    21181 gattaatggc catcggagta cccataatgc ctaagccaat aaatcccagt ttcataatta
    21241 acctcttttt aaatgtcgct attcctgaat tcagacaaca cgatcccctg ccaacttagc
    21301 agcctaaaaa agcattgtct gaatagcgtt acgttttaac ggtacggatc catccagcgt
    21361 aaaccggctt ccgtggtggt ttggggttta tattcacacc caacccaacc gttgtagtca
    21421 gaattttcga ttaccttaaa gagataatca taattaattt ctccggtccc cggttcgccg
    21481 cgatgcggat tatcggcaat ttgcaggtga ccaattttat cagcccattg cgtcatagtg
    21541 ttggttaatt caccttccat ccgctgcatg tgataaatgt catactgaat tttcaaattg
    21601 cagcaaccaa catcatcaat caatttcagc gcctgccgag ttccggtgag atggaaacca
    21661 ggaatatcaa aatggttaat aggttcaatc agtaataaaa tatcttcttt catcagcata
    21721 ttcgcggcat aacgcaggtt ttctacaagc gttgcgtgaa tctgttcact gctgaaacca
    21781 gccggcgttt taccgaccag acagttaata tttttattac ccagcgcacg ggcataacga
    21841 atcgctgctg ctacgccatc ccgaaactct tcttcacggc caggaataca ggcaataccg
    21901 cgctcccccg ccgcccagtc acccgccggt aaattgtgca gcgtatgttc gagtttatta
    21961 ctcgccagca cctgttttaa ttcttcaatg tcgtagtcat aaggaaacat aaattcaacg
    22021 ccacgaaaac cacactgcgc agctttctca aaacgggcga gaaaatcata ttcaccaaat
    22081 aacatcgata aattagcaga gaaacgtaac attatttatc tcccttattc atagtgcatg
    22141 aagcaggttt cagtcggtgc atccgctgcg ttatcggcga tatcttcaaa ttccatgacg
    22201 ttatccagtt cgctgcccat cgaaatattt gtcacacgct cgagaataac ttccacgact
    22261 accggtaccc gatattgcgc cattaaggct ttcgcctgtt caaaggccgg tgcaatatct
    22321 tccggtttga agacgcgaat agctttacaa cctaaacctt ccgctacttt tacgtggtcg
    22381 acgccgtaac cgttcacttc gctggagttg atattctcga aagcgagttg cacgcagtag
    22441 tccatgtcaa aggcgcgttg tgactgacga atcaggccga gataggcgtt attcaccagc
    22501 acatggatgt acggaatgtt gaactgcgcg ccaacggcta actcttcaat caggaactgg
    22561 aagtcgaagt caccggaaat cgccaccaca ttgcgtttcg gatcagcggc acaaaccccc
    22621 agcgccgccg gaatcgtcca gcctaacgga ccagcctgac cacagttgat ccagtggcgg
    22681 tctttaaaga catgcagcat ttgtgccgca gcgatttgtg acaggccaat ggtggtgaca
    22741 taacaaacat cgcgaccaaa ggctttgttc atctcttcat acacgcgctg cggtttcacc
    22801 ggcacgttgt cgaaatgggt tttgcgcagc aaagtgcgtt tgcgctgctg gcaatcggcg
    22861 acccattctt tacgacacgg cagacgaccc gctttttgca tctcctgcgc cacttcaacc
    22921 agcagtgtca gcgccgcttt agcatcagag acaatgccga gatccggaca cagcacgcgg
    22981 ccaatttgcg tcggctcaat atcgatatga acgattttgc gcccttcggt gtatttctct
    23041 accgaaccgg tatgacggtt agcaaaacgg ttaccgatac caaacaccat gtcggacgcc
    23101 agcaacgttg cgttaccgta acgatgcgcg gtttgcagac cgaccatccc ggccatcagt
    23161 tcatgatcgt ccgggataca gccccagccc atcagcgttg ggatcaccgg aacgctggtc
    23221 agttcagcaa actgttgtaa cagtgcagct gcgtcagcat tgattacccc gcccccggca
    23281 acaatcaccg gacgttcggc ctggattaac atttctacag ctttttcgat ctgcatacgg
    23341 ctggcagcag gtttgtagac cggcagcggt tcgtacatgt caggatcaaa ctcgatttcc
    23401 gcaacctgaa cgtcgaacgg taaatccacc agtaccggac ccggacgacc agaacgcatc
    23461 aggtgaaatg cctgttgcag cacgcgaggc accagcgccg cttcacgaac tgtaaccgcc
    23521 attttgctga ccggtttagc aattgcttca atatctacgg cctgaaaatc ttctttatgc
    23581 agacgggcgc gcggtgcctg gccggtaatg cacagaatag gaatggaatc agcagaagcg
    23641 gaatagagcg cggtgatcat gtccgtgccc gcaggaccgg aagtccccag acatacgccg
    23701 atattccctg ccgttgcgcg ggtataacct tccgccatgt gcgaagcacc ttccacatga
    23761 cgcgccagaa tgtgacgaat accgccgtgc ttacgcatcg ctgagtagaa cggattgatt
    23821 gcagctcccg gaacgccgaa ggcggtggtg ataccttctt tctccagcac atacattgcc
    23881 gcgtcaacgg ctctcatttt tgccatttta ttcctacctc tattaattgg aaaaattttc
    23941 caactttcaa attttgtcaa aaaaacccgc tccgcagagc gggatgcgac agacactatg
    24001 gatgtgcttt cagtcccaac gccgtgctga tatcgcgggc ggtgtctctg accagctcac
    24061 cctgactgac aaaacgatct tctgtcagtc ttgatgaagg cccggagatg gagatagcgg
    24121 caacaacgct acctacatca tcgtaaattg ctgaagctat gcaattcaga cctacaacat
    24181 gctcttcttt atctacggta tagcccagtt cacgcgcttg ttccaggtcc ttcagcaggg
    24241 tgggcatatc cacaagcgta gttggcgtaa actgctgcaa accggtttgc agaatgatgc
    24301 tcatcaactc ctcttccgcc agcggataaa gcagcgcttt gcccgcaccg gaagcatgca
    24361 gtggcagacg actgcccagt ggcgcgcaca tcctgaccat cgatttacac tctaactgac
    24421 caattaatac cgcttcattg ccgttacgga tcgcgacatt gaccgtttcg ccggaaagta
    24481 acatcaggcg gcgcataaac ggcccggcga cggagaggac atcgcggtta tggatgtacg
    24541 ccgcaccgac gttaaagaca cctaatccta tatgccacca gcctaactga ctgtcctgat
    24601 agacaaaatc cgctgcctgt aaaaccttca gcaagcgaaa ggtcgtggag agcggcaaat
    24661 ccagattgag agaaatatcg ctaaccgacg aacttccccc acttttttcc agatattgca
    24721 gaatcgcaat tccccgctct aatgcctgtg cgcccttctg tgccacaggc tccgcctgtc
    24781 ctggcctgcc gcgccgtcta acttccgtca tacctacgtt aactccgacc tacaagtgag
    24841 cataaacaat gaacaattag tttatcttat gcaagtcggt tgcaggcgtc acgcaaaaca
    24901 gagttcctgt tcaggaatac tttcaacatc acagttgtca ctgccgccgc gatcgatggt
    24961 cagaaaatcg gtgacgcgct gccaggcgaa aagtgggtga tgccagacgt tacgatggta
    25021 attcactccc tgttcgccgt tggtaataaa cgcccgcagg gttgacaggt ctggtttgtc
    25081 gtcacctaac gctacgacca ccacaaacac ctcacctttc atcgggataa aggcctgagt
    25141 acccagcgga tgacgttcga gttcgtgaat ggtcagcggc agattcgccg gttgcgctcg
    25201 gttaatgctg ataagcgtac ggtcttgctc aagaatctca accagcgcca aatcgtggta
    25261 acgctccacc aggccattgt taatatggaa aaaatcccgt tgctgcgttt cgattacgtc
    25321 gccataagca ctaaaggctt cctgacttaa cggtaatacc tgaagtttca tctttttgtc
    25381 cttgtaacat aattccgttc aaccagcgcc gaccggtcag cttggtttca ataacaggca
    25441 atctaaaacc ttaagagaac aacatcaaca caaaacttgg aatatttttc caaaactaga
    25501 tttagtccac aaattgaaca gggttatcat gtaaaaatac tgataaatca gaggataaga
    25561 aaatcacgca acttgcggat ttaaaagggg atacctgaag atgttcgatc cagaaacctt
    25621 gcggactttc attgcggttg ctgaaacagg aagtttttca aaagcggcag aacgattatg
    25681 taaaaccacg gcgacgatca gttatcgcat taaacttctg gaagagaata ccggagtggc
    25741 gctgtttttt cgcacgactc gtagcgtaac gttgacagcg gctggcgagc atctactttc
    25801 ccaggccaga gactggctga gctggctgga aagtatgccc agcgagctgc aacaggtgaa
    25861 tgatggcgtg gaacgccagg tgaatattgt catcaacaac ctgctctaca acccccaggc
    25921 cgtcgcccag ttgctggcgt ggctgaatga acgttacccc tttacccagt ttcacatctc
    25981 ccgacaaatc tatatgggcg tctgggactc gctattgtac gaaggttttt cactggctat
    26041 cggcgtcacg ggaaccgaag cgctggcaaa tacctttagt cttgatccct taggatcggt
    26101 gcaatggcga tttgtcatgg cggcggatca tccgctggcg aacgttgaag agccgctaac
    26161 agaagcgcag ttgcggcgct ttccggcggt caatattgaa gacagcgccc gcaccttaac
    26221 caaacgcgtc gcctggcgat tgccagggca aaaagagatt attgttcctg atatggaaac
    26281 gaaaatcgcc gcccatctgg cgggcgttgg cattggtttt ttgccaaaat cgctttgcca
    26341 gtcaatgatc gataatcaac aactggtcag ccgggtaatc ccaacgatgc gccctccttc
    26401 gccattgagt ctggcatggc gcaaatttgg cagcggcaaa gcggtagaag atattgtgac
    26461 cttgtttacc cagcgcaggc cggaaatcag cggattttta gaaattttcg gcaacccacg
    26521 cagttaaaaa tcaagtttcc ttgcgcgttg gcgcgtaaaa tagcgcgcaa gctgatttga
    26581 ggttcacttt taactatgca agagagacac acggaacagg actatcgtgc cctgctgatt
    26641 gctgatacgc ccattattga tgttcgcgcc cctatcgagt ttgagcaagg cgcaatgccc
    26701 gccgctatca atctgccgtt aatgaataac gatgaacgcg ccgccgttgg cacctgctat
    26761 aaacagcaag gctcagacgc agcgctggcg ctgggacata aactggttgc gggtgaaatt
    26821 cgtcagcagc gcatggacgc ctggcgggca gcctgcctgc aaaatccgca aggtattctc
    26881 tgctgcgccc gtggcggtca gcgctcacat attgtgcaaa gctggttgca tgcagcgggg
    26941 attgattatc cgctggtgga aggcggttat aaggcactgc gccagaccgc gattcaggcg
    27001 actattgaac tggcacaaaa accgatagtg ctgattggcg gttgtaccgg cagcggtaaa
    27061 acgctgttag tgcagcaaca gccgaacggt gttgatctgg aagggttggc gcgtcatcgc
    27121 ggttcggcgt ttggtcgcac gttacaacca caacttagcc aggcgagttt tgaaaacctg
    27181 ctggctgccg aaatgctaaa aaccgacgcc cgtcagaatt tgcgcctgtg ggtgctggaa
    27241 gacgaaagcc ggatgatcgg ttcgaatcac ctgccggaat gcctgcgcga gcgaatgact
    27301 caggcggcga ttgcggtggt agaagatccg tttgagatcc gtcttgagcg cctgaacgaa
    27361 gagtatttct tgcgtatgca tcatgatttt acccacgcgt acggcgacga acagggctgg
    27421 caggaatatt gcgaatacct gcatcacgga ctttcggcta ttaagcgtcg gctggggcta
    27481 cagcgatata acgaactggc tgcaaggctg gatgcagcac tgacaacgca actcaccacc
    27541 ggcagcaccg acggtcatct ggcctggctg gtgccgttac ttgaagaata ttacgacccg
    27601 atgtatcgct atcagcttga gaaaaaagcg gaaaaagttg tctttcgcgg tgagtgggcg
    27661 gaagtggcgg aatgggttaa ggcgcagtaa tgagggacgt atttgtatta gtcgcgactt
    27721 gatctgacac atggccttga aaggttgaga gttaccggtt ttgatatgag tgtcgaatcc
    27781 ttatccaaaa caagaggtaa ctctcatgct tcatactgcc aatcccgtca tcaaacacaa
    27841 agccggtttg ctcaatctgg ctgaagaact cagcaacgta tcaaaagcct gtaaaatcat
    27901 gggcgtttcg cgtgatacgt tttatcgtta ccgcgaactg gtcgctgaag gcggtgtgga
    27961 tgcgcagatt aatcgtagtc gccgcgctcc taaccttaag aaccgtaccg atgaggcaac
    28021 tgaacaggct gttgttgatt acgccgtcgc tttcccgaca catggtcagc accgggccag
    28081 caacgaactg cgtaaacagg gggtttttat ctccgacagt ggtgtccgtt ctgtctggtt
    28141 actccataac cttgagaatc tcaaaagacg atactgaaca aattttatca ggtgacgttc
    28201 cgtaaaaagt tgtatggagt tctcgatgca gtcggcgcga ctatcagtac ccccgctgaa
    28261 ccctggacta tcgggccgcc gtactggggc atgtcaccct ggcgtgccgc tggttttccg
    28321 ctaatggttt atttcctctt tctttcccgg caagaattta ttttaatccg gaaggattat
    28381 ttattttcag atagggactc acattaaaca cgtaacattt taattaatgc atggctgaat
    28441 atgtattaca caatgtcaga tatcgtaaaa gaccagtgca attctatgta aactctctga
    28501 ctgttcattt tatttgttgt ttcagggtcg gaactcaccc tcaataaaca actgaatacg
    28561 agtagatatt catcgtctga gctatatggc tttacacaat agccgacatg tcttttctca
    28621 gtcaggaatt ttacttttcc tcactacgaa gttgacgact tcgatatggg atagactctt
    28681 aattcaagca attactctgc acaagcatca ggcaatccag ctccaatatt tgttataaaa
    28741 taatgcgtaa cattaatttc accaacaaaa aaaggtttac accatcccct tgccctggta
    28801 tcttaccata tttacaccac actttttttg tatctggatc attctttgat acgattttcg
    28861 ccacttgttc cctggccctc actgtcaatg ttttcttaaa cctcccccca tcatcatttg
    28921 ttagggaaaa ataagaaaaa aataatcatc ttctacattt gtgtatattg atatatccac
    28981 cgctacgtgc ggaaaaaaat ctgggagcca tgtaaaaact ccatcatttt ctggtattgc
    29041 catatagttt atgtaatgtt tacttacaag agattcatct ttgtataaat aaagataagt
    29101 aattacgcat aaaacaacaa tgattataat agcaataata aatattatca tctttgatag
    29161 attacttgag atagccagca tcttgtaaag cctttatcgt ttctttatgc tctggattaa
    29221 tataatcact acatctatct gagcaatctg ttgttgatgg acatgtcaac ccatggtcat
    29281 ttacagccaa gtctttttta ttatcttcaa tcatttcaga atgggatagc tttacttttc
    29341 tgccgtacat accgaacaga tttaacccgt aatctctaat ctctttttca taacccagcc
    29401 ctttcgccga tcggctaaca ccagcgtcat ttaatttaga cactcgacaa aatgccatac
    29461 aatggaaaaa ttgatcagtc ccacctaaat ttaatctctt catatcttca taagtatcag
    29521 ataatatatc ccatgctctt tgatggttta gttgatcttt tcttcttatt agcgctacat
    29581 ctgcgggact taaccctaat ggatcaatac cattcaccgg attcagcgga tacgcataca
    29641 gactccatcc cccctccagc cctatcgggt cctgagtgat ataccgcccc tgcaacggat
    29701 cgtagtaccg gttccggttg tagtacagcc ccgactcctt atcatactgc tgccccggca
    29761 gccggtacgg ctggtgcagg tgatgcgggt tctcctcatt cagctggttg ccccattcat
    29821 catactcccc gctccacgcc gtattgccgt cttcgctgat gagcgccagc ggcagacccc
    29881 ggtggtcgca gtggtaaaga tgaacttttc tcgccggtgt gtattccggc tccacctgtc
    29941 tggccagttg ctccaccgtc agcccgcact gcgcaagcca cgcccggctt tcactgctca
    30001 cgcggtctgc ccggatttct tcctccagtc tgtccagcag ccgcaccagt tcagccggaa
    30061 ataccacacc gtgcccgtcc tcgctccctt cctgctgcag cttctccgcc aggctgcggc
    30121 gctgcgcttt ctcccgctca ccgttgtctg tttcgatgcg gatgagcggc gcgaagcttc
    30181 ccggctcgta taccgtctgg atacgcgtgg tgtcggtctg taccgtcgtc agccggtcgc
    30241 cgtcccaccc gtaccacgtc acctccggtt tccgcgacag cgacatccat cccgtcaggt
    30301 cacgcccgcg acgccacacc cgtttccccg tccggcg
//
LOCUS       DAZFBR010000054        29950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFBR010000054 DAZFBR010000000
VERSION     DAZFBR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.054"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(167..1996)
                     /gene="glmS"
                     /locus_tag="ACHJZ2_002606"
     CDS             complement(167..1996)
                     /gene="glmS"
                     /locus_tag="ACHJZ2_002606"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO4568690.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2158..3528)
                     /gene="glmU"
                     /locus_tag="ACHJZ2_002607"
     CDS             complement(2158..3528)
                     /gene="glmU"
                     /locus_tag="ACHJZ2_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO4568691.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3880..4299)
                     /gene="atpC"
                     /locus_tag="ACHJZ2_002608"
     CDS             complement(3880..4299)
                     /gene="atpC"
                     /locus_tag="ACHJZ2_002608"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO4568692.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4320..5702)
                     /gene="atpD"
                     /locus_tag="ACHJZ2_002609"
     CDS             complement(4320..5702)
                     /gene="atpD"
                     /locus_tag="ACHJZ2_002609"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO4568693.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5729..6592)
                     /gene="atpG"
                     /locus_tag="ACHJZ2_002610"
     CDS             complement(5729..6592)
                     /gene="atpG"
                     /locus_tag="ACHJZ2_002610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO4568694.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6643..8184)
                     /gene="atpA"
                     /locus_tag="ACHJZ2_002611"
     CDS             complement(6643..8184)
                     /gene="atpA"
                     /locus_tag="ACHJZ2_002611"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO4568695.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8197..8730)
                     /gene="atpH"
                     /locus_tag="ACHJZ2_002612"
     CDS             complement(8197..8730)
                     /gene="atpH"
                     /locus_tag="ACHJZ2_002612"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO4568696.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8745..9215)
                     /gene="atpF"
                     /locus_tag="ACHJZ2_002613"
     CDS             complement(8745..9215)
                     /gene="atpF"
                     /locus_tag="ACHJZ2_002613"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO4568697.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9277..9516)
                     /gene="atpE"
                     /locus_tag="ACHJZ2_002614"
     CDS             complement(9277..9516)
                     /gene="atpE"
                     /locus_tag="ACHJZ2_002614"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO4568698.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9563..10378)
                     /gene="atpB"
                     /locus_tag="ACHJZ2_002615"
     CDS             complement(9563..10378)
                     /gene="atpB"
                     /locus_tag="ACHJZ2_002615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO4568699.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10387..10767)
                     /gene="atpI"
                     /locus_tag="ACHJZ2_002616"
     CDS             complement(10387..10767)
                     /gene="atpI"
                     /locus_tag="ACHJZ2_002616"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO4568700.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11384..12007)
                     /gene="rsmG"
                     /locus_tag="ACHJZ2_002617"
     CDS             complement(11384..12007)
                     /gene="rsmG"
                     /locus_tag="ACHJZ2_002617"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO4568701.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12071..13960)
                     /gene="mnmG"
                     /locus_tag="ACHJZ2_002618"
     CDS             complement(12071..13960)
                     /gene="mnmG"
                     /locus_tag="ACHJZ2_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO4568702.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14339..14782)
                     /gene="mioC"
                     /locus_tag="ACHJZ2_002619"
     CDS             complement(14339..14782)
                     /gene="mioC"
                     /locus_tag="ACHJZ2_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO4568703.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14872..15330)
                     /gene="asnC"
                     /locus_tag="ACHJZ2_002620"
     CDS             complement(14872..15330)
                     /gene="asnC"
                     /locus_tag="ACHJZ2_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO4568704.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15482..16474
                     /gene="asnA"
                     /locus_tag="ACHJZ2_002621"
     CDS             15482..16474
                     /gene="asnA"
                     /locus_tag="ACHJZ2_002621"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO4568705.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16479..17930)
                     /gene="viaA"
                     /locus_tag="ACHJZ2_002622"
     CDS             complement(16479..17930)
                     /gene="viaA"
                     /locus_tag="ACHJZ2_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO4568706.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17924..19420)
                     /gene="ravA"
                     /locus_tag="ACHJZ2_002623"
     CDS             complement(17924..19420)
                     /gene="ravA"
                     /locus_tag="ACHJZ2_002623"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO4568707.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19643..21511
                     /gene="kup"
                     /locus_tag="ACHJZ2_002624"
     CDS             19643..21511
                     /gene="kup"
                     /locus_tag="ACHJZ2_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO4568708.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21678..22097
                     /gene="rbsD"
                     /locus_tag="ACHJZ2_002625"
     CDS             21678..22097
                     /gene="rbsD"
                     /locus_tag="ACHJZ2_002625"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO4568709.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22105..23610
                     /gene="rbsA"
                     /locus_tag="ACHJZ2_002626"
     CDS             22105..23610
                     /gene="rbsA"
                     /locus_tag="ACHJZ2_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO4568710.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23615..24580
                     /gene="rbsC"
                     /locus_tag="ACHJZ2_002627"
     CDS             23615..24580
                     /gene="rbsC"
                     /locus_tag="ACHJZ2_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO4568711.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24605..25495
                     /gene="rbsB"
                     /locus_tag="ACHJZ2_002628"
     CDS             24605..25495
                     /gene="rbsB"
                     /locus_tag="ACHJZ2_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO4568712.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25621..26550
                     /gene="rbsK"
                     /locus_tag="ACHJZ2_002629"
     CDS             25621..26550
                     /gene="rbsK"
                     /locus_tag="ACHJZ2_002629"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO4568713.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26554..27546
                     /gene="rbsR"
                     /locus_tag="ACHJZ2_002630"
     CDS             26554..27546
                     /gene="rbsR"
                     /locus_tag="ACHJZ2_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO4568714.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27512..28939)
                     /gene="mdtD"
                     /locus_tag="ACHJZ2_002631"
     CDS             complement(27512..28939)
                     /gene="mdtD"
                     /locus_tag="ACHJZ2_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO4568715.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(28962..29654)
                     /gene="yieP"
                     /locus_tag="ACHJZ2_002632"
     CDS             complement(28962..29654)
                     /gene="yieP"
                     /locus_tag="ACHJZ2_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO4568716.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7221 a         7668 c         7653 g         7408 t
ORIGIN      
        1 tttgtcaatg attcgaggta tttaattact actacggtta aaacaagtgt tcaacggtgc
       61 cccaaataat agctgaagtt aattatatgg aatgaggtta gattattaat gctggtaagt
      121 aaaagggccg ccaaaaggca gcccttcgtt cagcaaatga ataatattac tcaaccgtta
      181 ccgattttgc caggttacgc ggctggtcaa cgtcggtgcc tttgatcagc gcgacgtgat
      241 aagccagtaa ctgcagcgga acggtgtaga agatcggtgc aatcacctct tccacatgcg
      301 gcatctcgat gatgtgcatg ttatcgctac ttacaaaacc cgcatcctgc tcggcgaaga
      361 catacaattg gccgccacgc gcgcgaactt cttcaatgtt ggatttcagt ttttccagca
      421 attcgttgtt cggcgcaacg acgataaccg gcatatcggc gtcaatcagc gccagcggac
      481 cgtgtttcag ttcgcctgca gcgtaggctt cagcgtgaat ataagagatc tctttcagct
      541 tcaatgcgcc ttccagcgcg attgggtact gatcgccacg gcccaggaac agcgcgtgat
      601 gtttgtcaga gaaatcttct gccagagctt caatgcgttt gtcctgagac agcatctgct
      661 caatacggct cggcagcgcc tgcagaccat gcacaatgtc atgttcaatg gaggcatcca
      721 gacctttcag gcgagacagc ttcgccacca gcatcaacag cacagttaac tgagtggtga
      781 atgctttagt ggatgccacg ccgatttccg tacccgcgtt ggtcatcagc gccagatcgg
      841 attcgcgcac cagagaagaa cccggaacgt tacagattgc cagcgaacca aggtaaccca
      901 gctctttcga cagacgcagg cccgccaggg tatccgcggt ttcgccagac tgtgacaagg
      961 tgatcatcag gctgttacga cgcacggcag atttacgata gcggaattca gaggcgattt
     1021 cgacgtcgca cggaatacct gccagcgatt caaaccagta acgggaaacc ataccggagt
     1081 tataagaagt accacaggcg aggatctgaa tatgctcaac cttcgacagc agttcgtcgg
     1141 cgttcggtcc cagctcgctt aaatcaacct gaccgtggct gatgcgcccg gtaagggtgt
     1201 ttttgatcgc gttcggctgt tcgtagatct ctttctgcat gtagtgacgg taaatacctt
     1261 tatcgcccgc gtcatattgc agattggatt cgatatcctg acgttttact tccgcgccag
     1321 ttttatcgaa gatgtttacc gaacggcgag tgatttccgc aatatcgccc tcttcaagga
     1381 agataaagcg acgggtcacc ggcagcagcg ccagctggtc agaagcgata aagttttcac
     1441 ccatacccag accaatcacc agcggactac cagaacgtgc tgccagcagg gtatccgggt
     1501 gacgggagtc catgatcact gtaccgtacg caccacgcag ttgcgggata gcacgcagaa
     1561 cagcctcacg cagagtaccg ccctgtttca gctcccagtt caccagatgg gcaatcactt
     1621 cggtgtcggt ttcagaaacg aaggtatagc cacgcgcttt taactcttcg cgcagcggtt
     1681 catggttttc gatgatgccg ttatgcacca ccacaatgtg ttcagaaaca tgcggatgcg
     1741 cattcacttc tgaaggttca ccgtgggtcg cccagcgagt gtgagcaata ccggtgccgc
     1801 catgcagagg atgttcttcc gctgcctgag ccagcatctg gactttaccg aggcgacgca
     1861 ggcgggtcat atgaccttcc gcatcaacaa cggccagacc ggcagagtca tatccgcggt
     1921 attccagacg acgtaaacct tcaagaagga tttctgctac atcacgttgc gcgatcgcgc
     1981 caacaattcc acacatagtt tttgattccg atttatatcg ttgtcggtca acctgtatgc
     2041 ccgttttttc gggcgccccg agccttgtag agagtggggt tatttttata gttactgctt
     2101 gtgggaggga gattatgtta tctcctcatc ccatgtgacc ggattacccg gccagaatca
     2161 ctttttcttt accggacgac gccagccttc tttctgagtc tgcggcacac ggctgatagc
     2221 taatgcattt tcgccgacat tacgcgtcac agttgtaccc gcagcaatgg tcgcgccttt
     2281 gcctactgtt accggggcca ccagctgagt gtcggaaccg acaaacacat cgtcgccgat
     2341 aatggtctta aatttattcg caccatcgta gttgcaggta atggttcccg cgccgatgtt
     2401 aacgttatcg ccaatttccg catcgccaag gtaagtcaga tgaccagctt tcgagccttt
     2461 acccagacgt gcttttttca tctcaacaaa gttaccgacg tgtgcacctt ccaacaactc
     2521 agcaccagga cgcaaacggg caaacgggcc aatggtacag gccgccgcca gattcgcgtc
     2581 ttccacgacg gtatatgggc taatttcgca atcatcgcca atcacgctgt ttttaatcac
     2641 gcaaccggtg ccgattttca cgcgatggcc gagagtcacg ttgccctcga tgataacgtt
     2701 agtatcaatt tcaacatcgc gcccgtgagt aagcgtaccg cgcagatcaa aacgcgccgg
     2761 atcgcgcagc ataacgcctg ctaacagcag tttttcagcc tgttcggact ggtaaacgcg
     2821 ctccagacgg gagagttgca ggcggttatt cacgccttct acttcgctta aacgttgcgg
     2881 atgaacggcg acgatttcac gcccttcctg atacgccagc gcaataatgt cggtgatgta
     2941 gtattcgccc tgcgcattat tgttggtcag cttcgccagc cagcgtttca tatctgcgcc
     3001 gttagcgatc agaatgccgg tgttgatctc ctgaatctga cgctgctcgt cggtggcatc
     3061 tttgtgctca acaatgccgg taactttgcc gttttcacgg gtgatacgtc cataaccggt
     3121 cggatcatcc agtttcaccg tcagcagacc aatgccaccc tgcggtttag catcacgcag
     3181 acgctggagt gtttcgacag agatcagcgg cacgtcgccg tagagcatta aaatgtcttc
     3241 atcatcggca aagaaaggtg cggcctgctg catggcatga cccgtaccca gctgctctgc
     3301 ctgaagcacc cagttcaggt tgtcgtcttt cagcgcctgt tttagcagat cgccaccgtg
     3361 accgtacacc aggtgaacgt gcgctgcgcc taattcattc gcagcatcaa tgacatgctg
     3421 aaccatcgct ttcccggcaa gggtatgcag cactttcgga agatcggaat acatgcgcgt
     3481 gcctttacct gcggcaagga tcactacgct catagcatta ttcaacatac gcgtcctgac
     3541 tgtaatttga gaacgaattt aaaccgcttc accttgaaaa aactacattt ttttcatcgt
     3601 gaaatggaca gaggataaat tgttcaataa cggattatcc ccgctgacga aagcgccatt
     3661 ttcgaccatc gtcacgcctt ttgtctgcag aaaattaagg cagtaccttc atttaagcgt
     3721 caggtggatg tttttgctct tatttcgatc aatgaataaa cgggaaaaca gggggtttta
     3781 tatcagcagg atctatgtga acgctattca ggacgggtca cacgcgcaaa aaaaagccag
     3841 cctgtttcca gactggcttt tgtgcttttc aagccggtgt tacatcgctt ttttggtcaa
     3901 ctcgataacg cgcagctgcg cgatcgcttt ggccagttcc gcagacgcct gagcgtaatc
     3961 tacgtcgccg tgagagctgc taatgtgctc ttcagcctta cgtttcgctt ccatggctcg
     4021 cgcttcgtcg agatcctggc cgcgaattgc ggtgtcggcc agaacggtca cgttgccagg
     4081 ctgcacttca agaatgccgc cagacagata gataaactct tcgtgaccgt gctgtttcac
     4141 gatgcgaatc ataccaggct taatggcggt gagcagcggt gcgtggccag ggtagatccc
     4201 cagttcacct tcgctacccg ttacctggat tttctcgacc agaccagaga acatttgttg
     4261 ctctgcgctg acgacgtcca ggtggtaagt cattgccata tcaccctccg attaaggcgt
     4321 taaagttttt tggctttttc cacagcttct tcgatggaac cgaccatgta gaacgcctgc
     4381 tccggcaggt gatcgtattc gccttccatg atgcctttaa agccacggat ggtgtctttc
     4441 agggagacgt atttacccgg agaaccggtg aatacttctg ccacgaagaa cggctgggac
     4501 aggaagcgct ggatcttacg agcacgcgct accaccagtt tgtcttcttc agacagttca
     4561 tccataccca ggatggcgat gatgtctttc agttcctgat aacgttgcag gatggactga
     4621 acgccacgcg cggtgtcgta gtgttcctga ccaaccacca gcgggtccag ctgacggctg
     4681 gtggagtcca gcgggtcaac ggccgggtag atacccagag acgcgatctg acggctcagt
     4741 accacggttg cgtcaaggtg cgcaaaggtg gttgccggag acgggtcagt caagtcatcc
     4801 gcaggtacgt atactgcctg tacggaggtg atagaaccgg ttttggtgga ggtgatacgt
     4861 tcctgcagaa cgcccatctc ttccgccagg gtcggctgat aacctaccgc tgaaggcata
     4921 cggcccagca gtgcggatac ttccgtaccg gccagggtgt aacgatagat gttgtcaacg
     4981 aacagcagaa cgtcacgacc ttcgtcacgg aatttctcag ccatggtcag accggtcaga
     5041 gctacgcgca gacggtttcc cggcggctcg ttcatctggc catacaccag ggatacttta
     5101 tcgataacgt tggagtcggt catttcgtgg tagaagtcgt tcccctcacg agtacgttca
     5161 cctacgcccg caaacacaga gtaaccggag tgctcgatcg cgatgttacg aatgagctcc
     5221 atcatgttta cggttttacc tacacccgca ccaccgaaca gaccaacttt accgccctta
     5281 gcgaacggac acatcaggtc gataactttg ataccggttt ccagcagttc ctgagagttt
     5341 gacagctctt cgtaggaagg tgctgcgcgg tgaatcgccc aacgctcttc ttcaccgatc
     5401 tcgcctttca tgtcgaccgg ttcacccagt acgttcatga tacggcccag agtcgcttta
     5461 cctaccggga cttcgatcgg gtgttcgagg tcttttacat ccagaccgcg acgcagaccg
     5521 tcggaggaac ccattgcgat ggtacgcacg ataccgccgc cgagctgctg ctgaacttcc
     5581 agcaccagac gctcattacc attttgcacc tcaagagcat cgtacacgcg cggtacggca
     5641 tcctgaggga attcgacgtc aactacggcg ccgattacct ggacaatctt tccagtagcc
     5701 atcttaaatc ctctacgaaa taacctgttt aaaccgcggc ggcccccgag acgatctcgg
     5761 tgagttcctg agtaatgctg gcctgacgag ctttgttgta taccaactgc agctctttaa
     5821 tcaggctgcc gccattgtcg gtcgcggctt tcatcgccac catacgggcg gcctgctcgc
     5881 tggccaggtt ttcaaccacg ccctgataaa cctgagattc gacataacga cgcagcaggg
     5941 tatccagcaa cgccttcgga tcgggttcgt acaggtaatc ccaggattta tgtttcagat
     6001 catcatcatc tgatgccggt aacggcagca gctggctgat ggtcggaacc tgagacatgg
     6061 tgttaataaa tttgttgctg acaatgtaaa gcttgtccag acggccttcg tcgtaggcct
     6121 gcaacatcac ttttaccgga ccgatcagtt cggacaggga agggttatcc cccatgccgg
     6181 tgacctgggc aacaacattg ccgcccacgg agttgaagaa cgacacgcct ttcgagccga
     6241 tcattgcgag gtcgcattga acgcctttgt cggtccaggt cttcatttcc gccagcagtt
     6301 ttttgaacag gttaatgttc aaaccaccgc acaaaccacg gtcggtcgac accaccaggt
     6361 agcccacgcg tttaacgtcg cggtcttcca ggtaagggtg cttatattcc agattaccgt
     6421 gtgcaaggtg accaatcact ttgcgcatgg tttctgcata aggacggctg gccgccatgc
     6481 gatcctgcga tttacgcatt ttggaagcgg cgaccatctc catcgcttta gtgatctttt
     6541 gcgtgttctg gacgcttgcg atcttactac gtatctcttt tgcgccggcc atgagcttct
     6601 cctcaatgcc ttgcggcctg ccctaaggca agccgccaga cgttaccagg attgggttgc
     6661 tttgaaggaa tcgaggatgc ctttcagctt gccttcgatt tcgtcgttgt agccaccggt
     6721 ctggttgatc tcttgcatca acggagcgtg atcacggtcg acgtaagcca gcagagcggc
     6781 ttcgaagctg ccgattttcg acagttcaac atccgccagg taaccacgtt ctgctgcgaa
     6841 cagaaccaga gactgctgcg caacggacat cggcgcatac tgtttctgtt tcagcagttc
     6901 ggtcactttt tgaccgtggt caagctgctt acgtgttgca tcgtcaaggt cggatgcaaa
     6961 ctgagagaac gctgccagtt cacgatactg tgccagagcg gtacggatac caccggacag
     7021 ttttttcatg atcttggtct gtgctgcacc accaacacgg gatacggaaa tacccgggtt
     7081 aaccgcagga cgaataccgg cgttgaacag gttggtttcc aggaagatct gaccatcggt
     7141 aatggagatt acgttggtcg gaacgaacgc agaaacgtca cccgcttgag tttcgataat
     7201 cggcagcgcg gtcagtgaac cggttttccc tttcacttca cctttggtga aagcttcaac
     7261 gtattcggcg ttaacacgcg cagcacgctc cagcagacga gagtggaggt agaatacgtc
     7321 gcccgggaat gcttcacgtc ctggcggacg acggagcagc agcgagatct gacggtaagc
     7381 aacagcctgt ttagacagat catcgtaaat gatcagcgca tcttcaccgc ggtcacggaa
     7441 gtattcgccc attgcgcaac cggcatacgg tgccaggtat tgcagtgcag cggattcaga
     7501 cgcggttgct accacaacga tggtgttagc cagtgcgccg tgctcttcca gtttacgtac
     7561 cacgttagaa atggtggacg ctttctggcc gatagcgaca tagatacatt tgataccgga
     7621 atcgcgctgg ttgatgatgg catcgatagc cagtgcggtt ttaccggtct gacggtcacc
     7681 gatgatcaat tcacgctgac cacgaccgat tgggatcatg gagtcaacgg ctttataacc
     7741 ggtctgtacc ggctgatcta cggactgacg ttcgataacg cccggagcga ttgcttctac
     7801 agcagagaag ccgtcgtgat ccagcggacc tttaccgtcg attggtgcac ccagagtgtt
     7861 aaccacacgg cccagcaggc cacggccaac cggaacttcc aggatacggc cagtacactt
     7921 aactttcatg ccttcggcaa ggtcagcata cggacccata acaaccgcac caacagagtc
     7981 gcgctcgagg ttcagtgcga tagcgtaacg gttacccggc agggagatca tttcaccctg
     8041 catacaatcg gccaggccgt gaatgcggat aacaccgtca cttacagaaa caatagtacc
     8101 ttcgttgtga gcttcactca caacattgaa ctgagcaatg cgctgcttga tcagttcgct
     8161 gatttcggtg gaattcagtt gcatgctcca gtccccttaa gactgcaaga cgtctgcaag
     8221 gcgctcaaga cgaccgcgta cgctgccatc aatgaccata tcacccgctc ggatgataac
     8281 gcctgccatt acagacttat cgattttgca attcagctta actttgcgtg acagacgttt
     8341 ttccatcgca gcagaaattt tcgcgagctg ttgttcactc agtgcggcag cggaaatgac
     8401 gtctacctca gcggtagcct cactcacggc acgaaggtga ataaactgct ccaaaacatc
     8461 cgggagcgcg ttaagacgac cattttcagc cattacccga ataaggttct gaccgttttc
     8521 gtccagttgc tcaccacaaa ctgcgataaa cgactcggca agcgtttctg gcgcaagcgc
     8581 gccagagaga agctctgcca tttgttcgtt tttggttacc tcggcggcaa acgccagcat
     8641 gtcctgccag cgttctacac tttggtgttc gacggcaaag tcaaaagctg ctttggcgta
     8701 ggggcgagct accgtaataa attcagacat cagcccctcc ctccttacag ttcagcgaca
     8761 agtttatcca cgatgtcgct gttagcagct tcatccacgg aacgttcgat gatcttctcg
     8821 gcgccagcaa cagccaggat agcaacttgc ttacgcagct cttcacgggc acgtttacgc
     8881 tcggcttcaa tttccgcctg cgcctgggcc acgattttag tacgttcctg ttctgcctca
     8941 gctttcgctt cgtccagaat ctgcgagcgg cgtttgttcg cctgctcgat gattacctgg
     9001 gcttccgctt tcgctttttt cagctggtcg gtcgcgctgg cctttgcaag gtcaaggtcc
     9061 ttatgtgctc gttctgcgga agcaaggccg tcagcaattt ctttttgacg tttttcgatg
     9121 gctgccatta atggcggcca tacgtacttc atgcagaaca gaacgaacag gacaaacgcg
     9181 atggcctggc cgaggattgt tgcgttaaga ttcacagcac aatgcctcta tttagttaac
     9241 gttctgatat tgctctttaa ataaaagcaa cgcttactac gcgacagcga acatcacgta
     9301 cagacccaga cctacagcga tcatcgggat agcatccacc agacccataa cgataaagaa
     9361 ctgagtacgc agcagaggaa tcagatcagg ttgacgcgct gcgccttcca ggaatttacc
     9421 cccgaggatg ccgataccga tcgcagcacc gattgccgcc agacccatca tcacagcggc
     9481 agccatgtac agcagatcca tattcaggtt ttccatgaca gtctccagtt tgtttcagtt
     9541 aaaacgtagt agtgttggta aattaatgtt cttcagacgc catcgacaga tagacgatcg
     9601 tcagaaccat gaagatgaag gcttgcagcg taatgatcag gatgtggaaa atggcccacg
     9661 gcacattcag gatccactgt gaccaccacg gcaacagacc agcaatcaga atgaaaatca
     9721 gctcaccggc atacatgtta ccgaacagtc gcaaaccgag tgaaactggt ttggacagca
     9781 ggcttacccc ttcaaggatt aagttgacag gaatgaacgc ccagtgattg aacggctgca
     9841 gcgtcaactc tttcgtgaag ccgccgatgc ctttcatttt gatgctgtag aacagaatca
     9901 ggataaatac gcccagtgcc atagacagcg ttacgttcac gtccgcagac ggaaccacac
     9961 gcagtgcagg cagacccagt acatgttcag caatgtacgg cagcaggtcg ataggcagta
    10021 aatccatcag gttcatcagg aatacccaga cgaagatcgt cagggccagc ggagcaatca
    10081 gcttgctttt gccatggtac atgtctttca cgctaccatt aacaaagccg atcaccagct
    10141 caatcgcggt ctgaaactta cctggcacac cgctggtcgc ctttttggct acgctacgga
    10201 ataaaaccag gaacaacaga cccagcacca ccgagaagaa catggagtca atattgattg
    10261 tccagaaggt ggctgggggg ttttgtggat ccaccagcga gaatgtacgc aggtccagct
    10321 gaaggttatt caggtggtgt cctatgtaat cctgcggcgt catattttct gaagccatga
    10381 tgccttttac cctttgttgt taattacagc cggtgccagt atctgaacca ccagcaccaa
    10441 aacccacgta acgatcagcg gcaagaatac cgcctttaaa accgccaacg ccaccaccag
    10501 taacaccaac atcgccagaa ctttgaaagc ttcaccaaat gcgaatgtcc aggccacccg
    10561 gcctttcgct ggtgtatgcg cctggtgacg ccaggcaaat atcataaaca aaacgttagg
    10621 cagaaagact gccaggcccc cgcttattgc agagacgccc cagaaggggt ctttgaggct
    10681 gaacagcaat ccacttgcta tcaccaccag taactgaacg agcagaagct tccgagcaac
    10741 gtttcgactc acgagcgaca cagacatcac gtttttcact cctgctccct tcgaggtatg
    10801 ccgcgtgtcg tataaaactt tctttaaggc ttagagtcaa gcatcaaaaa gcggtcaaat
    10861 tatacggtgc gcccccgtga tttcaaacaa taagtagcca aaaggtgaat aaatgtttaa
    10921 atatttttcc agtgcataca attgcgactt ttctactaac cctgttcgat catgaaaaac
    10981 tgtaaataac gcgtaaacac tggtgataaa gcgtgcttca gatcacatat tgcgcatgtt
    11041 cgcgcacagc atatttattt acttggcaaa tgatgccttt gcaagtttat gatatttcag
    11101 tctaaaaaca gatactgttt taataaatga catttacaca acaaaaacca cccattgaca
    11161 tttttaataa tgttttaaca gccaatgatg gttcttagcg ccgattttta gcagactgat
    11221 attttcacta atgacttatt ttctgcttac caaaaaaagc cacgttatct tgttgatgca
    11281 aaagagtgaa cgtggcgtta aatgtaacca gttatatcag tagaaaacct ggttgttgtt
    11341 aacagtctaa ccggtcaatt ttttatgatt tttttgataa aaattaaatt ttatttgctt
    11401 taatcaccac cagatgacgt tcgccatcca gggctggaac ctgaagttta accactgatt
    11461 cgacctgata ttcttcgggc aacaaagcga tttcatcttc cggcatttgc cctttcagcg
    11521 cgtagaaacg gccttgctca ccaggaagat ggtggcacca gctcaccata tcgttcagag
    11581 aggcaaaagc gcggctaatt acgccatcaa atggcggctc tgaaggaaac tcttctaccc
    11641 tgctctgtac tggctcaata ttctccagtt taagctcatg ttgcacctga cgaaggaaac
    11701 gcacgcgttt accaaggcta tccaacagag tgaaatgggc ttcaggacgc acgatagaga
    11761 gtggaatgcc aggcagtcca ggaccggtgc cgacatcgat aaaccgttca ccttgcagat
    11821 acggtgccac cacaatgcta tcgagaatat ggcgtaccag catctcatta ggatcgcgga
    11881 ccgaagtcag gttgtacgct ttgttccatt tatgcagcat attcacgtag gcaataagct
    11941 ggtttttctg gtgatcggta agcgaaatac ctgcgtcttt cagcagtaag gagagtttgt
    12001 tgagcacggt gattacctgt tcttgatgcg ttgcctggta agcgggggct taccaggcat
    12061 ttttaatgcg ttatgcgcta cgacgcagca taccctgttt tttcagccac accagcagaa
    12121 tggagatggc cgcaggcgtg acgccagaaa tacgtgaagc ctggccgata gaggccggtt
    12181 tgtgatcgtt aagtttggcg atcacttcgt tagaaagacc ggatacctgg cggtaatcca
    12241 gtgtcgcggg tagcaaggtg ttctcgttac gcagctgctt ttcgatctca tcttgctggc
    12301 gcgcgatata accttcgtat ttaacctgaa tctcaacctg ttccgccgcc tgttcgtctg
    12361 tcaacgcagg ggcaaacggc gtcagcgtgg ttaatttttc ataagtcatt tccggacgac
    12421 gcagcagatc ttcaccactg gcttcacggg aaagcggcgc agtcaggtga gcattcactt
    12481 cggctgcagc ttccgccgac ggggttaccc aggtcgattt cagacgctga cgctcacgct
    12541 cgatattctc aagtttctcg ttaaagcgcg cccaacgttc gtcatccacc aggcccagtt
    12601 cacgaccgat ttcagtcaaa cgcagatccg cattatcttc gcgcagcatc agacgatatt
    12661 ctgcgcgcga ggtaaacata cgatacggtt ctttggttcc taaagtgcac aggtcatcaa
    12721 ccagtacgcc gagataggcc tgagaacgcg ccggagccca accttctttg tcatcagaca
    12781 gacgggcagc gttaagaccg gccagcaaac cttgcgcagc ggcttcttcg taaccggtag
    12841 tgccgttaat ctgaccagca aagaacagcc cctggataaa cttgctctcc agcgtcggtt
    12901 tcaggtcgcg aggatcgaag aagtcatact caatggcata acccggacgc acgatcttcg
    12961 cgttttccat cccctgcata gagcggacga tttgcatctg cacatcgaac ggcaggctgg
    13021 tggagatacc gttcggataa atttcgttag aagttagccc ttccggttca aggaagatct
    13081 gatgctgatt tctgtcggcg aagcgcatga ctttgtcttc gatcgacggg cagtagcgtg
    13141 ggccgacacc ttcgatcacc cctgcgtaca ttgggctacg atcgaggtta ctgcggatca
    13201 catcatgggt tttctcgttg gtatgggtga tataacacgg cacctgctgt ggatgctggg
    13261 acgcattgcc cataaaagag aataccggca ttgggttatc gccatgctgt tgcgcaagca
    13321 cgctaaagtc gatggttcga gcatcaatac gcggtggtgt cccggttttc agacgaccaa
    13381 cgcgcagcgg aagttcacgc aaacggcgag aaagcggaat ggacggcgga tcaccagcac
    13441 ggccaccgct gtagttatcc agaccgatat gaattttacc gtcgaggaac gtcccaacgg
    13501 tgagcacgac ggctttggca cggaacttca gtcccatttg ggtaacagca ccgaccacac
    13561 gatcgttttc gacaataaga tcttcaaccg cctgctggaa gatcatcagg ttcggttggt
    13621 tctccagcgc cgtacgtacc gcctgacggt agagcacacg atccgcctga gctcgggtag
    13681 cgcgaactgc cggtcccttg cttgcgttta gtatcctaaa ctggataccc gcctgatcga
    13741 tcgctttcgc catcagaccg ccgagtgcat ccacttcttt taccagatgt cccttcccaa
    13801 taccgccgat cgccgggttg cagctcatct gccccagagt gtcgatattg tgtgtcaaaa
    13861 gcagagtctg ttgacccata cgcgccgcag ccatcgcggc ctcggtgcct gcatgacccc
    13921 cgccaatgat gatgacgtca aaaggatccg gataaaacat ggtgattgcc tcgcataacg
    13981 cggtatgaaa atggattgaa gcccgggccg tggattctac tcaactttgt cggcttgaga
    14041 aagacctggg atcctgggta ttaaaaagaa gatctattta tttagagatc tgttctattg
    14101 ttatctctta ttaggatcgc actgccctgt ggataacaag gatccggctt ttaagatcaa
    14161 caacctggaa aggatcatta actgtgaatg atcggtgatc ctggaccgta taagctggga
    14221 tcagaatgag gggttataca caactcaaaa actgaacaac ggttgttctt tggataacta
    14281 ccggttgatc caagcttcct gacagagtta tccacagtag atcgcacgat ctgtatactt
    14341 atttgagtaa attaatccac gatcccagcc attcttctgc cggatcttcc ggaatgtcgt
    14401 gatcaagaat gttgatcttc agtgtttcgc ccgtctgttt tgcaccggaa tttttgagtt
    14461 cagcctcgag tttatcgata gccccacaaa atgtgtcata ttcacgacta ccaataccga
    14521 ttgcgccaaa gcggaccgca gaaagatcgg gcttctgttc ctgcaatgct tcatagaaag
    14581 gagaaaggtt gtccggaata tctccggcac cgtgggtgga gctgataacc agccagatcc
    14641 ccgaggcaga taaatcttcc aacagcggac cgtgcagcgt ttcggtggta aaacccgcct
    14701 cttccagctt ttcagccagg tgttctgcta catattcggc accgccgagg gtgctgccgc
    14761 tgataagagt tatatctgcc ataaaccgcc acctttatta agagtggcgt attgtacgct
    14821 gtgaacgcgt tgggatctac ctgtggaaaa gtatgggatt aaaaaagccg atcagggctt
    14881 gatggtacgc atgatcgggt tctgcaggac gatcagtgtc tcggtggact gaatttcatc
    14941 aattgtttgg atcttgttga taagtacatg ctggagagca tcgatcgaac ggcacatcac
    15001 ttttataaag atgctgtagt ggccggtggt gtagtacgcc tcggtcactt catcgaggct
    15061 ttccagcttt gccagcgcgg aagggtagtc tttggcgctc tttaatataa tgccgataaa
    15121 gcagcctacg tcataaccga gctgcttcgg gctgacatca atacgcgccc cggtaatgat
    15181 ccccgcctgc ttcattttct ctactcgaac gtgaatcgtc cccggactga cgccaaattg
    15241 tttcgccagt tcggcgtaag cggtgcgcgc attgcccatt aatgcttcca ggatgccacg
    15301 gtccagattg tcgatcagat aattttccat aggattttct tatgcggatt gatgattcat
    15361 tctattttag ccttcttttt taatgaatca aaagtgagtt aggcttttta ttgaatgatt
    15421 attgcatgtg tgtcggtttt tgttgcttaa tcataagcaa caggacgcag gagtataaaa
    15481 aatgaaaacc gcttacattg ccaaacaacg ccaaattagc ttcgtgaaat ctcacttttc
    15541 tcgtcaactg gaagaacgtc tggggctgat cgaagtccag gcgccgattc ttagccgtgt
    15601 gggggatggc acgcaggata acttgtcggg ctgtgaaaaa gcggtacagg taaaagtgaa
    15661 agctctgcct gatgcccagt tcgaagtggt tcattcactg gcgaagtgga aacgtcagac
    15721 cttagggcaa cacgacttca gcgcgggcga agggctgtac acgcacatga aagcccttcg
    15781 ccccgatgaa gaccgtcttt ctccgttgca ctcggtctat gttgaccagt gggactggga
    15841 acgcgtaatg ggcgacggtg agcgtcaatt ctcgactctg aaaagcacgg tagaggcgat
    15901 ttgggcggga attaaagcaa ccgaagctgc ggttagcgaa gagtttggcc tggcaccgtt
    15961 cctgccggat cagatccact tcgtacacag ccaggagtta ctgtctcgtt atccggatct
    16021 tgatgccaaa gggcgtgagc gggcgatagc gaaagatctt ggtgcggtat tccttgtcgg
    16081 gattggcggc aagctgagcg atggtcatcg ccacgacgtg cgcgcaccgg attatgatga
    16141 ctggagcacc ccgtcagagc tgggccatgc gggtctgaac ggcgatattc tggtgtggaa
    16201 ccctgtactg gaagatgcgt ttgagctttc ttccatgggg atccgcgtgg atgccgacac
    16261 gttgaagcat cagttggcgc tgaccggtga tgaagatcgt ctgcagctgg aatggcatca
    16321 ggcgttgctg cgcggtgaaa tgccgcagac catcggcggc ggtatcggcc agtctcgttt
    16381 gaccatgctg ctgctgcaac tgccgcatat cggccaggtc cagtgtggag tatggccagc
    16441 tgctgttcgc gagagcgtcc cttctctgct gtaataattt atcgccgcca gcgtctgagc
    16501 aggcggcttc gcatcccggt atcaaagcgc cagatatgat cgaaaatgcg catgatgccg
    16561 ggtttgccgt gtgccgacat cgccacggca tgaaagcgat gctgatgtac ccgctgcagc
    16621 tctttcactt tactcgtcac gtcgtcaggc aaccgctggg cgataaaatc agaaatcacc
    16681 accgcatcgg catcaaacca ttccctgctt tgcaagcgtt ccataatggc gcgaaaacaa
    16741 ctggcaagat cggtgccgcc acgaaactgc tggcttaaaa aacggattgc ttgttcgatg
    16801 ccttgtgggc ctgaaagctc ataacggacg atctcggtgg aaaatagcat aatataacag
    16861 cgccggtttt cagcgagagc aatgcgcatc aaagccaggc agaacgcttt cgcacactgt
    16921 tcattaaagc cgcccattga gccggaagta tccacgcaga caataaacgg tccgcgcggc
    16981 tgttcgtcgt aatctttatg caccaccggg cgttcgatca ctttttcacg ccacgactca
    17041 ccgtgcaggc gataggtgag caactgtttt tccaccagcc gacggtaaaa ctcatactcc
    17101 agttccgtta tccctagtgt cgccagttct ggcggcagaa gacgtaaaat atcatcgctt
    17161 tgttgcagac catcaacctg ctcaggaacc gtcgccggtt cgcgcaccat ggtgcggaag
    17221 gtttccatct gcgcatcgtt gcgcggtatt gatttggctt cccgggaacg ccccagttgt
    17281 tctgccaggc gtttcagttc cggctgttcg ttaagaaatt caccgtattt cacaatcaac
    17341 tgatagtcgc cacgtttaag ctgaccggcg ctcatatccc acagacgacc agctgcggta
    17401 ttgttatctg cgagaatcgg ttcaagttgt ccgctcagcg tcatgcgttc ctgaacttca
    17461 ctcaacagtt gttcgcgttc ttcttctaat agctgttgat ttaacgttgt tgcttgcacg
    17521 atcagactta aacgccagcg ctggagaaaa agcgtgtgta acgctgaagt gatcgtgctg
    17581 ttagcatcga ccaactgtcg ggcttgttct gcccatgggg aattcagacg atgcagtaag
    17641 tccaggatct gtggtagctg cacaataaac tgcggcgtgg agaggagctg gctttgctga
    17701 tagcacatca cctcttcggt gagttccggc gggactcggg catctttcag ccgactgcgc
    17761 agcgcctcac gccagcgggg aacatcatca gtgattgccg ccttcagtcg tgggaatttt
    17821 tcaaagaaga ctgccagctg cggtgaggcc agcagcgcga tgatcatctc ttcgatcaat
    17881 ccctcttcgc tgacggccag catcacatta agcgtatcca gcgttagcat tgttgtgcct
    17941 ggcgaatctg tgcgccgaca tcctgtaggc tggcttcaat gcgacctaac cagtcgccag
    18001 ggataaacag acatctttgc tgttcgctga ataaagcgtg ttgcttgcgc cagtcgcttt
    18061 caagctcttc caattgttgt tttatttcac ctggcagacc ttcagccgat gaaccgggaa
    18121 gtgccagcgt actgccttgt aaacttacat cgcgtacaac aagatgttgg gcgctatcaa
    18181 cttccagatt cagtttctgg gcaaagccga taccgttcag tttgccgcga atttcaccac
    18241 ctttgctcag ccactgttcc agcgcgctac gctcaaagga gatatgaacc acttccatat
    18301 tatgcaattt taacggtttt tgcagcagca gagtcagagt ggaagcagta acgttaacag
    18361 gaagttgata ctgctgacga cggctgaaaa tgccgcccag acgaattacc gttaaggctg
    18421 ttttatcgct ttgttgctgc tggagttgca ggtgacgttg cacaatcgcg cccaggcggg
    18481 tcaacatccc ttgctgttgc caggcatgac cggtcatcaa tacatcaatt tgttgttgta
    18541 tcaaattcag gctttgcgcg tcataccaca ggcaatcttt cagcaaaatg agatctaccg
    18601 gggcaacagc actgcgaccg ctaaaaaagg cgctggcctg caataatcga atcgcttttt
    18661 tccagcgacg atccgagaca taaggcgcat ccggtaattt atccagttgc tggcgcagca
    18721 taaaaatcag ctcaaataca tgatcgggca gcgtaatttc accaatctct ttttgccagc
    18781 gttcatattc ttcatctgtg acctgcaagg aggctggaac cggattgtca ttttcatcct
    18841 gttgactggt cagcatggag cggaaattcg ctttatcctg cactttatct aaccacagac
    18901 gaatcagcat gcggtcatat aacgcttcca gactgctgtc tgcttccggc agctcgttgg
    18961 aggccgccac cagcaggcgc atcgggattt tttctacgtg tgcaccgttg cggaactggc
    19021 gctcgttaat ggcggtgagc agggtattaa gaattgccgg acccgctttc cagatctcat
    19081 ccagaaagac gatttctgct tccggcaggt aaccgctggt taaacgttca tagcgccctt
    19141 catcttttag cgcctgaata gaaaggggac caaaaacttc ttccggcgtg gagaagcggg
    19201 tcatcagata ttcaaacgcg cgggcattct gaaaggcgaa ttttaagcgc cgggcgatca
    19261 aacttttggc aatacctggc gggccaagga ggaacacact ttcaccactt aatgccgcta
    19321 ataaacacaa gcggatggcg tggctacgtt cataaagccc cttttccaac gaactgctca
    19381 ggcgggaaat tctttccgct aataaatgag ggtgagccat aatgaagtgg cgtcctttcg
    19441 tcaaaagttc tgcgtaaatt gcgagtatag acgtttcttg ctggtggcta aaatagtctc
    19501 aaaggggggg tatttttctt tgagccaggt taatgtggcc gcatttagga gtacgatttt
    19561 gccgttaatc gtgcatactg tgcgcttttt tgtgggccaa gggactaagc acacatttca
    19621 tatttcaacg aaagactagt ctatgagcac tgataataag caatcattgc ccgcgattac
    19681 cctcgcggcg attggagttg tctacggcga tattggtacc agcccgttat atacacttcg
    19741 tgaatgtttg tccggccagt ttggttttgg cgttgaacgc gatgccgtgt ttggcttttt
    19801 atcgctgatc ttctggctgc taatctttgt ggtttccatt aaatatctca ccttcgtgat
    19861 gcgggcagat aacgccggtg aaggggggat cctgacgttg atgtcgcttg ccgggcgtaa
    19921 tacgtcggcg cgaaccacat caatgctggt gattatgggg ctaatcggcg gcagcttttt
    19981 ctatggtgaa gtcgtcataa cacccgctat ttcggtgatg tcagccattg aaggtctgga
    20041 aatcgtcgcc ccgcagctgg atacctggat agttcccctc tcaattatcg ttctcacttt
    20101 attatttatg attcaaaagc atggcacggc tatggtcggt aagctgtttg cgccgatcat
    20161 gctgacctgg tttttgattc tggcaggact ggggttacgt agcattattg ctaaccagga
    20221 agtgctgtat gcgctgaatc cgatgtgggc ggtgcatttc ttccttgaat acaaaacggt
    20281 ttcttttatt gcattagggg cagtggtgct gtcgattacg gggggcgagg cgctgtatgc
    20341 tgatatgggg cactttggta agttccctat tcgcctggcg tggttcaccg tcgtattacc
    20401 ttccttgacc cttaattact tcggtcaggg agcgctgttg ttaaagaatc cggaagcgat
    20461 taagaacccg ttcttccttt tggcaccgga ctgggcgctg atcccgctgc tgatcatcgc
    20521 cgcactggcg acggtaatcg cctcgcaggc ggttatctct ggcgtctttt cattgacgcg
    20581 tcaggcggta cgtctgggat atttgtcgcc gatgcgcatt attcacacct ccgaaatgga
    20641 gtcagggcaa atctatattc cgtttgtgaa ctggatgctc tatgtcgcgg tcgtgattgt
    20701 gattgtcagc tttgagcact ccagcaacct ggcggcggcg tacgggattg cggtgaccgg
    20761 aaccatggtg ctgacgtcta ttctctcgac taccgtggca cgtcagaact ggcactggaa
    20821 taagtatttt gttgcgctga tcttgattgc tttcctttgt gtcgatattc cattgttcac
    20881 cgctaacctc gataaactgc tctccggcgg ctggttgcca ttgagcctcg gtacggtgat
    20941 gtttatcgtg atgaccacct ggaagagtga gcgtttccgc ttgctgcggc ggatgcatga
    21001 acatggtaac tctctggaag cgatgattgc ttcgctggag aaatcaccgc ctgttcgcgt
    21061 gcccgggacc gcggtatata tgtcgcgtgc aatcaacgtc attccctttg cgctgatgca
    21121 taaccttaaa cataacaagg tattgcatga gcgggtgatt ctgttaactc tgcgcaccga
    21181 agacgctcca tatgtccata acgtccgtcg ggtacagatt gaacaactgt cgcccacttt
    21241 ctggcgcgtg gtggcaagtt atggttggcg agaaacgcca aacgtggaag aagttttcca
    21301 ccgctgcggt ctggaaggat taagttgccg gatgatggaa acctccttct ttatgtcgca
    21361 tgagtcgttg atcctcggca aacgcccgtg gtatttgcgt ctgcgcggca agctgtactt
    21421 gctgctgcaa cgtaatgcgc tgcgtgcacc agatcaattt gaaatcccgc caaacagggt
    21481 tatcgaactg ggtactcagg tcgaaatcta acgccagacg cctcctttct tcataagggg
    21541 gcgtttttgt tttcatggtt aatcaccatg taaaacgttt cgaggttgat cacatttccg
    21601 taacgtcacg atggttttcc caactcagtc aggattaaac tgcgggtcag cgaaacgttt
    21661 cgctgatgga gaaaaaaatg aaaaaaggca ccgttcttaa ttctgatatt tcatcggtga
    21721 tctcccgtct gggacatacc gatacgctgg tggtgtgtga tgctggttta cccatcccca
    21781 aaagtacaac gcgtatcgat atggcattaa cccagggtgt accttctttt atgcaggtgc
    21841 tgggcgtcgt cacaaatgaa atgcaggtcg aggctgccat tatcgcggaa gagatcaaac
    21901 accataatcc gcaactccac gaaacgttgc tcactcacct tgagcagctg caaaaacacc
    21961 agggaaatac cattgaaatt cgttacacca cgcatgaaca attcaaacaa caaaccgcag
    22021 aaagtcaggc ggtaattcgc agcggagaat gttctccgta tgcgaatatc attctctgtg
    22081 ctggcgtgac gttctgaggc cgtcatggaa gcattacttc agcttaaagg catcgataaa
    22141 gccttcccgg gcgtaaaagc cctctcgggc gcagcgttaa atgtctatcc gggccgcgtg
    22201 atggcgctgg tgggcgaaaa cggcgcgggt aaatccacca tgatgaaagt gcttactggc
    22261 atctatactc gcgatgccgg tacgctttta tggctgggga aagaaacgac atttaccggg
    22321 ccgaaatctt cccaggaagc cgggattggg attatccatc aggaactgaa cctgatcccg
    22381 cagttaacca ttgccgaaaa cattttcctc ggtcgtgagt ttgttaatcg ctttggcaaa
    22441 attgactgga aaaccatgta tgccgaagcg gataaattgc tggctaaact taacctgcgc
    22501 tttaaaagcg acaagctggt gggcgatctt tccatcggtg accagcaaat ggttgaaata
    22561 gccaaagtgc tgagctttga gtcgaaagtc atcattatgg atgaaccgac cgatgcgctg
    22621 accgataccg aaaccgaatc cctgttccgc gtcatccgcg agctgaaatc gcaaggccgc
    22681 ggtattgtct atatctccca ccgcatgaaa gaaatcttcg agagttgcga tgacgttacc
    22741 gtttttcgtg acggacaatt tattgctgag cgcgaagtgg catcactgac cgaagattcg
    22801 ctgattgaga tgatggtggg tcgcaagctg gaagatcaat atccgcacct ggacaaagcg
    22861 ccgggagata tccgcctgaa agtcgataat ctctgcggac ctggcgttaa cgatgtctct
    22921 tttactttac gcaaaggcga aattcttggc gtctctggtt tgatgggcgc aggtcgtacc
    22981 gaactgatga aagtgctcta cggcgcacta ccgcgcacca gcggttacgt caccctggat
    23041 gggcatgaag tcgttacccg ttcaccgcag gatggcctgg caaacggcat tgtgtatatc
    23101 tccgaagacc gtaaacgtga cggtttagtg ttgggcatgt cagtaaaaga gaacatgtcg
    23161 ctgacagcgc tgcgctactt cagccgcgct ggcggcagtt tgaagcatgc cgatgaacag
    23221 caggctgtga gtgattttat tcgcctgttc aatgtgaaaa cgccgtcgat ggagcaggca
    23281 attggtctgc tttccggtgg caatcagcaa aaagtggcga ttgcccgcgg tctgatgaca
    23341 cgccccaaag tgttgatcct tgatgaacct acccgtggcg tagatgtcgg cgcgaaaaaa
    23401 gagatctatc aactgattaa ccagttcaaa gccgatggct tgagcatcat tctggtgtca
    23461 tcggagatgc cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat
    23521 ctcagcgggg aatttactcg tgagcaggcc acccaggaag tgttaatggc tgccgctgtg
    23581 ggcaagctta atcgcgtgaa tcaggagtaa aaaaatgaca acccagactg tctctggtcg
    23641 ccgttatttc acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct
    23701 gatcgcgatt gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat
    23761 tctccagcaa acctcagtga acgccattat ggcggtcggg atgacgctgg tgatcctgac
    23821 gtcgggcatc gacttgtcgg taggttctct gttggcgcta accggcgcag ttgctgcatc
    23881 tatcgtcggc attgaagtca atgcgctggt ggctgtcgct gccgctctcg cgttaggtgc
    23941 cgcaattggt gcggtaaccg gagtgattgt agcgaaaggt cgcgtccagg cgtttatcgc
    24001 tacgctggtt atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc
    24061 agtgaatacc ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc
    24121 gctgggcgta ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat
    24181 gctgcatcac acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac
    24241 gcgtctttct ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct
    24301 gctggcatca ctggccggga tcattgaagt ggcgcgtctc tcctccgcac aacccacggc
    24361 ggggactggc tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc
    24421 gggcggaaaa ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa
    24481 taatggattg aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt
    24541 gattttgctg gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt
    24601 gaatatgaac atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt
    24661 cagtgcgaat gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc
    24721 gttctttgta tcgctgaaag atggcgcgca gaaagaggcg gataaacttg gctataacct
    24781 ggtggtgctg gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac
    24841 cgttcgcggc accaaaattc tgctgattaa cccgaccgac tccgacgcag taggtaatgc
    24901 tgtgaagatg gctaaccagg cgaacatccc ggttatcact cttgaccgcc aggcaacgaa
    24961 aggtgaagtg gtgagccaca ttgcttctga taacgtactg ggtggcaaaa tcgccggtga
    25021 ttacatcgcg aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgctgg
    25081 tacatccgca gcccgtgaac gtggcgaagg cttccagcag gccgttgctg ctcacaagtt
    25141 taatgttctt gccagccagc cagcagattt tgatcgcacc aaaggtttga acgtaatgca
    25201 gaacctgttg accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc
    25261 gctgggcgcg ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt
    25321 tgacggtaca ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc
    25381 tcagctaccc gatcagattg gcgcgaaagg cgttgaaacc gcagataaag tgctgaaagg
    25441 cgagaaagtt caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta
    25501 atcaggttgt atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat
    25561 aaagaaaagc agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat
    25621 atgcaaaacg caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat
    25681 cttcaatctt ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt
    25741 ggcggcaaag gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt
    25801 attgcctgta ctggcgatga cagcattggc gagagcgttc gccagcagct cgccactgat
    25861 aacatcgata ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt
    25921 tttgttaatg gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgccctt
    25981 tccccggcgc tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc attattaatg
    26041 cagctggaat caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag
    26101 actatcgttg cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg
    26161 gtggacatta ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa
    26221 aatgatgaag atgcagcgaa ggcggcgcag gtactgcatg aaaaaggtat ccgtactgta
    26281 ctgattactt taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt
    26341 cctggattcc gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg
    26401 ttaatcacgg cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct
    26461 gccgctgcga ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag
    26521 atcgacgcat ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgc
    26581 ctggcgggcg tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt
    26641 gaagcgatta ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagct
    26701 ctggcgcgta gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt
    26761 accaatcctt tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt
    26821 tatagtctcg tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa
    26881 acgctgatgc aaaaacgcgt tgatggcttg ctgttactgt gcaccgaaac gcatcaacct
    26941 tcgcgtgaaa tcatgcaacg ttatccgaca gtgcctactg tgatgatgga ctgggctccg
    27001 ttcgatggcg acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg
    27061 caatatctga tcgataaagg tcatacccgt atcgcctgta ttaccggccc gctggataaa
    27121 actccggcgc gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac
    27181 attcctgatg gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgctatg
    27241 cgccaactgc tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg
    27301 gctgttggcg tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg
    27361 gtgattggct atgacgatat cgaactggca agctttatga cgccaccatt aaccacgatc
    27421 catcaaccaa aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc
    27481 cagccgaccc ttcagcaaca acgattacaa cttactccga ttctgatgga acgtggttcg
    27541 gcttagattt acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga
    27601 acattgctgc tgaagcaaca gtaataatgc ccattgtgat aaacgtatag tggaattgtt
    27661 cgacagtcgt tgtgccttcc attccttcat aaacgcgaag gacggccgca cttacagcta
    27721 cgcctaaact gatagacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt
    27781 tgtcatcggt cagatcggca agtgtgatgg tattcatcgc ggtaaactgc gtcgacatag
    27841 ccatccctaa tataaacaac ggcaagatca gcatccatat tgccattgcc ggtgattgca
    27901 aagagaactg agcgatcatt agcccaataa tcaccgtgat ccccactaat gtatggcgat
    27961 agcccagacg acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg
    28021 tcggtgccat catacagcca gcaataaacg cctgataacc aaatcctacc tgtaacatta
    28081 atggcataag gaacggtaca cagccggtcc ccagacgggt tgcaatattg cctacgatac
    28141 cgatcgagaa agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtgcgtc
    28201 gtgcatggag aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa
    28261 tccagctggc gacaatcttt tccccgaata gctctattcc gcttgagaag agaacaaggc
    28321 tgaggccaaa cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg
    28381 gcatatgttt gcgcgcgtaa agaaggcccg caatacctat ggggatattg attaaaaata
    28441 tccagtgcca ggttgcccag gtgaccagca cgccgccaag aatggggcct aaaattggcc
    28501 ccaccagacc cggcatggcg acaaaattca atactggaag aagttcatta cgaggataag
    28561 cgcgcagtaa ggccagccga gcaacaggca tcatcattgc gccgcctatc ccctgaataa
    28621 cccggaagac aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaatg
    28681 tgaacagact cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc
    28741 cgcttaccgg aataagcatc gccaccgtca gcgtataact gatgatggct gattgcatcg
    28801 cgagaggaga acgattaagg ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg
    28861 catcaagtgc ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gccatactgc
    28921 gcttcttttt atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt
    28981 agcagcgcct gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgact
    29041 atcgcctgat gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg
    29101 tgataaaccg aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca
    29161 tagatatgct catgccaggc catatcgact tcgatccagc gttcacggcg aaagttctct
    29221 tttaatgccg ccatttcggc cattaacgta ttgagatgcg ctttctgttc cgcggtgcca
    29281 accgttgctg ccagtaggca agcttgtggc tccagacaga tacgcataac cagaaagtga
    29341 tcgatgacct gatgaaagtt ctcttctgtc atccaccagg taagcaattc ctgatcaaga
    29401 aaattccagt tcgattgtgg catgacccgg gtaccaattc gcggtcgcgg taaaaccatc
    29461 ccttttgccg ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc
    29521 tcgcccagct caatctcacc gggcaaaatg gtgccgggtt catattcacc ttttaagatc
    29581 cgttgcgcca gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt
    29641 gcgcttaaag gcattactaa tcttcctttt tctttttatt cctccttagt atgccaccag
    29701 gaagtgtgat tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggc
    29761 ttttgctgaa aaaatgcgcg gtcagaaaat tattttaaat ttcctcttgt cagaccggaa
    29821 taactcccta taatgcgcca ccactgacac ggaacaacgg caagcacgcc gccgggtcag
    29881 cggggttctc ctgagaattc cggcagagaa agcaaaaata aatgcttgac tctgtagcgg
    29941 gaaagcgtat
//
LOCUS       DAZFBR010000055        29857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFBR010000055 DAZFBR010000000
VERSION     DAZFBR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.055"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            88..714
                     /gene="upp"
                     /locus_tag="ACHJZ2_002633"
     CDS             88..714
                     /gene="upp"
                     /locus_tag="ACHJZ2_002633"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO4568717.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            800..2089
                     /gene="uraA"
                     /locus_tag="ACHJZ2_002634"
     CDS             800..2089
                     /gene="uraA"
                     /locus_tag="ACHJZ2_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO4568718.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            2184..2885
                     /gene="hda"
                     /locus_tag="ACHJZ2_002635"
     CDS             2184..2885
                     /gene="hda"
                     /locus_tag="ACHJZ2_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO4568719.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(3023..3382)
                     /gene="arsC"
                     /locus_tag="ACHJZ2_002636"
     CDS             complement(3023..3382)
                     /gene="arsC"
                     /locus_tag="ACHJZ2_002636"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO4568720.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3403..4866)
                     /gene="bepA"
                     /locus_tag="ACHJZ2_002637"
     CDS             complement(3403..4866)
                     /gene="bepA"
                     /locus_tag="ACHJZ2_002637"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO4568721.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRNAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            5079..6140
                     /gene="yfgO"
                     /locus_tag="ACHJZ2_002638"
     CDS             5079..6140
                     /gene="yfgO"
                     /locus_tag="ACHJZ2_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4568722.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(6178..7026)
                     /gene="focB"
                     /locus_tag="ACHJZ2_002639"
     CDS             complement(6178..7026)
                     /gene="focB"
                     /locus_tag="ACHJZ2_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO4568723.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(7048..9060)
                     /gene="hyfR"
                     /locus_tag="ACHJZ2_002640"
     CDS             complement(7048..9060)
                     /gene="hyfR"
                     /locus_tag="ACHJZ2_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO4568724.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGVFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(9090..9503)
                     /gene="hyfJ"
                     /locus_tag="ACHJZ2_002641"
     CDS             complement(9090..9503)
                     /gene="hyfJ"
                     /locus_tag="ACHJZ2_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO4568725.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(9496..10254)
                     /gene="hyfI"
                     /locus_tag="ACHJZ2_002642"
     CDS             complement(9496..10254)
                     /gene="hyfI"
                     /locus_tag="ACHJZ2_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO4568726.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTRVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(10251..10796)
                     /gene="hyfH"
                     /locus_tag="ACHJZ2_002643"
     CDS             complement(10251..10796)
                     /gene="hyfH"
                     /locus_tag="ACHJZ2_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO4568727.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(10806..12521)
                     /gene="hyfG"
                     /locus_tag="ACHJZ2_002644"
     CDS             complement(10806..12521)
                     /gene="hyfG"
                     /locus_tag="ACHJZ2_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO4568728.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(12511..14091)
                     /gene="hyfF"
                     /locus_tag="ACHJZ2_002645"
     CDS             complement(12511..14091)
                     /gene="hyfF"
                     /locus_tag="ACHJZ2_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO4568729.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(14096..14746)
                     /gene="hyfE"
                     /locus_tag="ACHJZ2_002646"
     CDS             complement(14096..14746)
                     /gene="hyfE"
                     /locus_tag="ACHJZ2_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO4568730.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(14758..16197)
                     /gene="hyfD"
                     /locus_tag="ACHJZ2_002647"
     CDS             complement(14758..16197)
                     /gene="hyfD"
                     /locus_tag="ACHJZ2_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO4568731.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(16214..17161)
                     /gene="hyfC"
                     /locus_tag="ACHJZ2_002648"
     CDS             complement(16214..17161)
                     /gene="hyfC"
                     /locus_tag="ACHJZ2_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO4568732.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(17172..19190)
                     /gene="hyfB"
                     /locus_tag="ACHJZ2_002649"
     CDS             complement(17172..19190)
                     /gene="hyfB"
                     /locus_tag="ACHJZ2_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO4568733.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     LNAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(19190..19807)
                     /gene="hyfA"
                     /locus_tag="ACHJZ2_002650"
     CDS             complement(19190..19807)
                     /gene="hyfA"
                     /locus_tag="ACHJZ2_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO4568734.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(20060..20530)
                     /gene="bcp"
                     /locus_tag="ACHJZ2_002651"
     CDS             complement(20060..20530)
                     /gene="bcp"
                     /locus_tag="ACHJZ2_002651"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO4568735.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(20530..21102)
                     /gene="gcvR"
                     /locus_tag="ACHJZ2_002652"
     CDS             complement(20530..21102)
                     /gene="gcvR"
                     /locus_tag="ACHJZ2_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO4568736.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            21248..22126
                     /gene="dapA"
                     /locus_tag="ACHJZ2_002653"
     CDS             21248..22126
                     /gene="dapA"
                     /locus_tag="ACHJZ2_002653"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO4568737.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            22143..23177
                     /gene="bamC"
                     /locus_tag="ACHJZ2_002654"
     CDS             22143..23177
                     /gene="bamC"
                     /locus_tag="ACHJZ2_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO4568738.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            23390..24103
                     /gene="purC"
                     /locus_tag="ACHJZ2_002655"
     CDS             23390..24103
                     /gene="purC"
                     /locus_tag="ACHJZ2_002655"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO4568739.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            24271..25134
                     /gene="ypfJ"
                     /locus_tag="ACHJZ2_002656"
     CDS             24271..25134
                     /gene="ypfJ"
                     /locus_tag="ACHJZ2_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO4568740.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            25149..27164
                     /gene="tmcA"
                     /locus_tag="ACHJZ2_002657"
     CDS             25149..27164
                     /gene="tmcA"
                     /locus_tag="ACHJZ2_002657"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO4568741.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATSAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            27238..27936
                     /gene="ypfH"
                     /locus_tag="ACHJZ2_002658"
     CDS             27238..27936
                     /gene="ypfH"
                     /locus_tag="ACHJZ2_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4568742.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(28017..28217)
                     /gene="ypfN"
                     /locus_tag="ACHJZ2_002659"
     CDS             complement(28017..28217)
                     /gene="ypfN"
                     /locus_tag="ACHJZ2_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO4568743.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(28245..29372)
                     /gene="dapE"
                     /locus_tag="ACHJZ2_002660"
     CDS             complement(28245..29372)
                     /gene="dapE"
                     /locus_tag="ACHJZ2_002660"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO4568744.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIALMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(29376..29732)
                     /gene="yffB"
                     /locus_tag="ACHJZ2_002661"
     CDS             complement(29376..29732)
                     /gene="yffB"
                     /locus_tag="ACHJZ2_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO4568745.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQKFFHEV"
BASE COUNT         7254 a         7808 c         8023 g         6772 t
ORIGIN      
        1 aagtcaacga aaataatatt gccgccttga agaaaggagg tataatccgt cgattttttt
       61 gtggctgccc ctcaaaggag aaagagtatg aagatcgtgg aagtcaaaca cccactcgtc
      121 aaacacaagc tgggactgat gcgtgagcaa gatatcagca ccaagcgctt tcgcgaactc
      181 gcttccgaag tgggtagcct gctgacttac gaagcgaccg ccgacctcga aacggaaaaa
      241 gtaactatcg aaggctggaa cggcccggta gaaatcgacc agatcaaagg taagaaaatt
      301 accgttgtgc caattctgcg tgcgggtctg gggatgatgg acggtgtgct ggaaaacgtt
      361 ccgagcgcgc gcatcagcgt tgtcggtatg taccgtaatg aagaaacgct ggagccggta
      421 ccgtacttcc agaaactggt ttctaacatc gatgagcgta tggcgctgat cgttgaccca
      481 atgctggcaa ccggtggttc cgttattgcg accatcgacc tgctgaaaaa agcgggctgc
      541 agcagcatca aagttctggt gctggtagct gcgccagaag gtatcgctgc gctggaaaaa
      601 gcgcacccgg acgtcgaact gtataccgca tcgattgatc agggactgaa cgagcacgga
      661 tacattattc cgggcctcgg cgatgccggt gacaaaatct ttggtacgaa ataaagaata
      721 aaaataatta aagccgactt taagagtcgg cttttttttg agtaaagcgc ctataacaca
      781 taatacagag gataatacta tgacgcgccg tgctatcggg gtgagtgaaa gaccgccact
      841 tttacagaca atcccgctta gtttgcaaca tttgttcgcc atgtttggtg caaccgtcct
      901 ggtgcccgtc ttatttcata ttaacccggc gactgtactg ttatttaacg gtattggaac
      961 gctgctgtat ctcttcatct gtaaagggaa aattccggct tatcttggtt ccagctttgc
     1021 ctttatttca ccggtattgt tactgttgcc gttagggtat gaagtcgcgc tgggcggctt
     1081 tattatgtgc ggcgtgctgt tctgcctggt ttcttttatc gtgaagaaag cggggaccgg
     1141 ctggctggac gtgctgtttc cacctgcggc aatgggcgca atcgttgccg tcatcggtct
     1201 ggagctggcg ggcgtagctg ccggtatggc gggtttactc ccggctgaag ggcaaacgcc
     1261 agactccaaa accatcatca tctctattac caccctggcg gtcacggttt taggttccgt
     1321 gctgttccgt ggtttcctgg caattatccc gattttaatt ggcgtgctgg tggggtacgc
     1381 gctctctttc gcaatgggaa ttgtcgatac cacgccgatt attaatgctc actggtttgc
     1441 gctgccaacc ctctatacgc cgcgcttcga gtggtttgcc attctgacta ttctgccagc
     1501 ggcgttagtg gttattgccg aacacgtagg gcacctggta gtaacggcta atatcgtcaa
     1561 aaaagatctg ctgcgcgatc caggtctgca ccgttcgatg tttgctaacg gcttgtcgac
     1621 cgtgatctcc ggcttctttg gctccacgcc aaacaccacc tacggggaaa acattggcgt
     1681 gatggcgatc acccgtgttt acagtacctg ggttatcggc ggggcggcga ttttcgctat
     1741 cctgctttcc tgcgtcggta aactggctgc cgctatccag atgatcccat tgccggtgat
     1801 gggcggcgtt tcgctgctgc tttatggtgt catcggtgct tccggtattc gtgttttgat
     1861 cgaatcgaaa gtggattaca acaaagcaca gaacctgatc ctgacttccg tgattttgat
     1921 catcggcgtc agtggcgcga aggtaaacat tggcgcggcg gagctgaaag gtatggcgct
     1981 ggcgaccatc gtcggtatcg gcttaagttt gattttcaaa ctgattagtg tgctgcgccc
     2041 tgaagaagtg gtgctggacg cagaagacgc ggatataaca gacaaataat gtaccgaccg
     2101 ggcagtgttg ctgcccggtt caaacatcat gggattctgt ggtaaacttc tcgcgatttt
     2161 gtgaaatcct ggttgaggta tctctgaaca caccggcaca gctctctttg ccactttatc
     2221 ttcctgacga cgaaaccttt gcaagtttct ggccggggga taactcctct ttactggccg
     2281 cgctgcaaaa cgtgctgcgt caggaacata gcggttacat ctatctctgg gcacgcgaag
     2341 gcgcggggcg cagccatctg ctgcacgcgg cttgcgcgga attgtcgcag cgtggcgatg
     2401 cggtgggcta tgtcccgctg gataaacgca cctggtttgt tccggaagtg ctcgacggta
     2461 tggagcattt gtcgctggtc tgtatcgaca acattgagtg tattgcaggc gatgagttgt
     2521 gggagatggc gattttcgat ctctacaatc gaattctgga atcgggcaaa acacggttgt
     2581 tgatcaccgg cgatcgtcca ccgcggcagt tgaatctggg attaccggat ctcgcgtcgc
     2641 gactcgactg ggggcagatc tacaaattgc agccactttc tgatgaagat aagttgcagg
     2701 cgctacagtt acgcgcgcgt ttgcgtggtt ttgaactgcc ggaagatgtg gggcgtttct
     2761 tgctgaagcg gctcgacaga gaaatgcgca cgctatttat gacgttggat cagttggatc
     2821 gtgcgtcgat taccgcgcaa cgtaagctga ccattccgtt tgtgaaagaa attctgaagt
     2881 tgtagataag gtgtttattg tcggatgcgg cgcgaacgcc ttatccgaac tacgcgaagt
     2941 taatccatag aattcgtagg ccggataaga cgtggcaagc gtcgcatccg gcaatgtgct
     3001 tcaactagcg atggagcaca tcttaaccca cgatttccag tacctgctcc ggtggacgac
     3061 caatccgcgc tttgccattc gcgaccacaa tcgggcgctc catcagcttc ggattatcca
     3121 ccattgcctg aatcagcgcc tcttcgctaa gcgaactgtc agccaggttc agttctttat
     3181 aaagatcctc tttctggcgc atcaattcac gggcgctatt catccccagc attttcagca
     3241 aatcgcgcag cgttgccgca tcggcgggtg tctcaaggta gagcaccact tctggctcca
     3301 cgccgttttc tttcagcagg ttcagcgttt cccggctctt tgaacagcgt gggttatggt
     3361 agatttttac ctgtttggtc attgttcttc ctttaatgcg aattacatct tggtataagg
     3421 cttaaagcgt tcctgcagct ggcgcaactg gtcgatgcgc gcatcgtaac gcgcttgttg
     3481 caggctgcct aatttcacct gcgaactggc gctactcaac agcgaaatgg cctgatcgag
     3541 tcgtccggcg agcgcataac cttctgctcg cgcagccagc tcctgatcgc ggttatttag
     3601 cgcggcttcc gcctgtgcca gcaaatccca gccgttgctg tcatctttat tattaaaggt
     3661 gtagcgatta agaatattgg ccgcttcttg tggttgaccg ccttgtagat aagcgttcgc
     3721 caggttgagc tgcaacacag gattggtgcg caaatcgcgg gcatttttca gacgattgat
     3781 cgcctcattg gctttgtttt gcccaagatc gatatcagta gccagatcga gataccatgc
     3841 gttgccaggt tctgccgcca gtaacggttg cagcgttttt cgcgcctcgt cgtatttatt
     3901 ggcttccatc gcctgtaaag cacgaccata ttgcgccgct cgttgctgac gaacgtttcc
     3961 tttcgcccat tcatccagca aatcactggt gagctggtta cgtccggaat tatacatccc
     4021 cagtgtgcgc gctttcgcca gatagaaatc ttccgacgac tgcaccacca tcgggcgcat
     4081 ctgattagca cggttgcggg catctgccag acgactttcc ggcaacgggt gagtcagtaa
     4141 aatttccggc gggcgcgagg agtaacgcgc ctgatcgagt aatttttcga ggaaggttgg
     4201 catcgcctgc ggatcgaatc ccgagcgttg cagcacctga ataccaatgc ggtccgcttc
     4261 ctgttcattt tgctgggtga aactgatcat cccctgacgc gttcccgcca gtgtaccggt
     4321 cagcgccgcc atccccgcct gcggactggc catcgccagt aaaatagaac ctaacgcgcc
     4381 gacccaggtc agcggcgcgt tgcgctgctg atcttccatc gctcgcgcca ggtgacgttg
     4441 ggtgacgtgg gagatttcgt gcgccataac tgaagccagt tgactttcgt tatcggaata
     4501 acggaacagg gcagagtgca gcaccacgtt gccgccaaag aaagcaaagg cgttaatttc
     4561 gtcgttgttg atcagaaaga aatgaaacgg tgtcttaacc gaattggcat gcgaaaccag
     4621 acgcatcccc agcgaattaa tatattgcgt taacagcggg tcattaatta acggcgcgct
     4681 gccgcgtagc tggcggacat aatagtcgcc catctgcatt tcctgaccaa tggaaagcgt
     4741 gcttcctgcg gaggttccca tatccggcaa ggtgtctgcg ctgtcggcaa atgccggggc
     4801 tacctgacca atggtcatag cagcaatgag ggttgcaacc aggttttttt tcaactgcct
     4861 gaacataacc tctatcctgt atttctgaag atagtcattt gaccgatact gtgaaatatc
     4921 gttcccaccg cgccgccttt gagtgtaacg gcgaagatta gcccactcaa atgacttttc
     4981 agtttcctga atccgtcata aaaagcgaag tcttttttgt gacatttcga tacaattgcg
     5041 gatcgcaatc ccgctcttga ggttcaggga aggtctttat gctcgaaatg ttgatgcaat
     5101 ggtatcgccg ccgttttagc gacccggaag cgattgcctt gctggttatt ttagttgccg
     5161 gatttggcat tatctttttc tttagtggcc tgcttgctcc gttgctggtg gctattgtgc
     5221 tggcctattt gctggaatgg ccaaccgtgc gcctgcaatc tattggctgc tcccgccgct
     5281 gggcgacgtc gattgtattg gtggttttcg tcggtatatt gctactgatg gcgttcgtgg
     5341 tactgcctat cgcctggcag cagggcatct acttaatccg cgatatgccg gggatgctca
     5401 ataagctttc tgactttgcc gccacgttgc cgcgccgcta tccggcgtta atggatgcgg
     5461 gcattattga tgcaatggcc gaaaatatgc gcagtcggat gctgaccatg ggcgattcgg
     5521 tggtgaaaat ttccctcgcc tcgctggtcg gtttgctgac cattgccgtc tatctggtgc
     5581 tggtaccatt gatggtcttc ttcctgctga aagacaaaga gcagatgctg aatgccgttc
     5641 gccgggtgct gccgcgtaac cgtggactgg caggacaggt gtggaaggag atgaatcaac
     5701 aaatcaccaa ctatatccgc ggcaaagtgc tggagatgat cgtagtaggg atcgccacct
     5761 ggctgggatt cttactcttc gggctgaact attcgctgct gctggcggtg ctggtcggct
     5821 tctcggtact tattccgtac attggcgcat ttgtggtgac cattccggtg gttggcgtgg
     5881 cgctattcca gtttggtgca ggcactgaat tctggagctg tttcgcggtg tatctgatca
     5941 ttcaggcgct tgacggcaac ctgttagtac cggtgttgtt ctccgaagcg gttaacctgc
     6001 atccgctggt gattatttta tcggtggtga tcttcggtgg tttgtgggga ttctggggcg
     6061 tattcttcgc cattccattg gcgacgctga tcaaagccgt gatccacgcc tggcccgatg
     6121 ggcaaatagc gcaagaataa tcttcagccc gacgaactgt ttcgccgggc tgatttttca
     6181 ggattcctga cgtaaataaa tagcccgata acaaatactc accagcactg caccgccgat
     6241 aatattaccc agcatcaccg ggagcagatt ggcggtaata aaatggctga ccgtcagtgc
     6301 aggaaaattg tctgcgctac tgtgcgccag ttgccagaag gaggtagggg cgaaatggcg
     6361 aatggcaatg gcgaacggaa tcacaaacaa attggcgata cagtgctcaa agccactggc
     6421 gacaaacagg gtgatgggca aaatcatggc gacgattttg tcgcataacg aacgcccgca
     6481 ataactcatc cacagcgcca ggcaaaccat cagattgcac ataatgccga ggctgacaga
     6541 ttcagtaaat gtatgatgca ttttgccctc ggcgcagtgt aaaaccgcga ctccccacat
     6601 cgcgttttca ctcatcacca gcccggaaaa ccagattaac aaactgaaac aggcaatacc
     6661 tgccagatta ccgcaggcca ccagaagtgc gttaatcagc caagttcgcc aactaataac
     6721 gccccgactt tttgccatca ccgtcattac cgacgaggtg aacaggctgg tgccgcaaac
     6781 cgccagcaaa ataaagccga gtgtaaagca aaggccgccc accagatggg ttaatgcctg
     6841 tgaagacggg gcatcggcga taacggaaag gtaaaaagta aatccgatcg ccataaacac
     6901 gccagcggac atcgccatta aaaagaccga cactttactg cgggcaattt tatgggcggc
     6961 aatccgttca gcgattgccg cttttctggc gctcaactgc aagtcgaaag agagtttgtt
     7021 tctcatgcat actcctgcaa aagcagatta caacacctcg cgaaccgaga tccccagacg
     7081 ctgcattcgt gacagcagcg tggtgcgctt catccctaat cgtgtcgccg cgccacgggg
     7141 cccggcaaca atgccattgg tttctcgcaa tacctgaaca atgcgctgac gttcttcttc
     7201 gtcattctct ggcgtcgtcg ggtgcagcaa ctgcgccatt gaactgcgaa gcgctgaatc
     7261 ttcatttagc gtcggcagta aacggctttg tcggacatta agatgtaaat tcagactgtt
     7321 accgcgagtc agcagtaccg ctcgctcaat cacgttttcc agctcgcgca cgttgcccgg
     7381 ccaatcccac gacatcaact ggcgtagtgc ctcggtcggg atggcgtcaa ttgcgcgatt
     7441 catatggcgc gccatttttt gcgtgaaatg ttttgctaaa agagggatat cttccggacg
     7501 gtctcgcagc ggcggcaatt ccagtgggaa gacattcagg cgataaaaga gatcgctgcg
     7561 aaactggcga tcttcaacca tttgccacaa atcacggttg gtggcggcaa tgactctgac
     7621 attcaccggg atcgttctac tcccgccgag acgctcaatt tcccgctcct gcaatacgcg
     7681 cagcaattta ggctgaagtt ctaacggcag atcgccaatt tcatcgagaa acaacgtacc
     7741 gccatcggca atttcaaaac gaccacgatg ggtattaatc gcaccagtaa agaccccttt
     7801 gtcatgaccg aataactcac tttccagaag actggcgggg atggcagcgc agttgatttt
     7861 gaccagcggc ttgtcgcgtc gcgggctaag ttgatggatc gctctggcga tcacctcttt
     7921 gccggttccg gtttcaccac aaatcaacac cgtactgtcg ctcttcgcca caatatctac
     7981 ctgctggagc aggtcttcca ttgcctggct ttgatagata atgtcaccga cgcccagatt
     8041 cgaaaggagc tgctcgctaa gctggtggtt ttcttgctgc aaactttcct gcaaatcggt
     8101 catgctacgc caggcatcgg cattgccaac ggcaatagcg atgcgatcgg ctatgtgttg
     8161 tagtagctgg cagttttcct cactaaagag agtggtagag gtatgcgcca gcaacaatgc
     8221 acccggtgta tggttgccaa aggtaagcgg tatcaggagc gaagattcgc agccgttaga
     8281 tgcaagtaag tgtaataacg ggtcgcgttg ccagagaaac agatcgtctg cacggtgcgt
     8341 caaggtcggt tgtcgggttt gtagcgtttg tgtcagcaat acactgtcgc caggcatatt
     8401 gcgtggcaga cacgcacaat gtgaggcaga aagatcgctg caccacaggc tgaacttctc
     8461 gttctttcga tgatcgccca gtaccatact gactgaagcc agaccgaaaa aatgatggat
     8521 ctcacgagcg acgtcagcga tcagatcgtc gagatcaaga tgtgacagca ctgcattagt
     8581 gatatccacc agtacccgat gatgatcgcg atctttctcc agcaaacgca gcgccacctg
     8641 ttgttcttca tcattgagcc atgattgcag aaccggggtg ataatgcgtg ccagccagtt
     8701 aaacagtgtg caatcctggg gagtaaaacg actgccgtgg gtgcgaataa agcgtagctc
     8761 cgccattcca ctactgccga gaaggacggc gtgacgcgaa taatcgacca tctcgccctg
     8821 atactctgtt gttgccgtca catcgctgcc atcatcatgg tagcggtaat agaagttgct
     8881 tttgagaaca tgcagttcaa taagttgtac cgggctgaac ggcggcggca gcgggatgaa
     8941 ggcgcgtaat aaagacgcct tgccgtgttt ctgcgctaac cgctctgcga gcattccgtt
     9001 taccgcttca attgttattc cttgcggcgg ggcaaacatc gcctcgtctg acatagccat
     9061 caacagtcct tattaatgag aaatccttct cacgcaattt ttctggccat cagatagata
     9121 gccggttcca gcacaatttc atccaacaga tgaatgaatt gaatgctacg cgcttgccac
     9181 acagacgtcg cgtcatctgc cagtggcgcg aatgcccggg tcaacagggc ggtgtggctg
     9241 gcgtcaataa caatctcacc aaaagtcatc acccccagca tcttacgtcg ggcttgctcc
     9301 tcttcgacca gtgcaagcca atcttcgtat tccgtcagtg ggcagcggaa ggtgacattc
     9361 agacaatcaa tcacgccaac gtgatgaccg atagccagcg agtaatacat tacctgggag
     9421 ctgtgttccg gcatctcgtc gctactggcg acaaactttt ttcgcagcgt ccagaaaaca
     9481 atttccccgc actcttcagt catgtaaccc ccggagtacg cgaaagagtt gctgaacgat
     9541 actattgaga cgtggatcgt cggcatcgcg caaccaggtg ttaacccgat ttcctgtagg
     9601 atcgttgctt aaatggcgca ggagccgatc gcaaatttct tgcccctgac gatagcccgc
     9661 cagccgccgc gcttctcgct caatggcgat acgctgtgat ggcgggatct gcggccacaa
     9721 cggttgcata gtcaccctgg tgggatcgcg ataatccacc gcgtgaatct tctgttgcag
     9781 caaaccgagc gccacggcga aaccgtgaat ggtggcggcc ggtgttggcg ggcagccggg
     9841 gatccaaaca tcaatgggga caatggtgtc gctgccgccc cagacgctgt agagatcgtg
     9901 gaaaataccg ccgccgacac cgcacgcgcc gtaggaaaca cagattttat gatcgggggc
     9961 agactcatac gcccgaagtg caggcatacg catcgcccgg gtgactgcgc cagtaaataa
    10021 caaaatatcg gcgtgacgcg gtgatgaaac aaccttaatg ccaaaacgtt ctgcgtcgaa
    10081 tacgggtgtg atggcggcaa aaatttcgat ttcacaggcg ttgcagccgc cgcaatcgac
    10141 gcgataaacg taggctgagc gacggatatc ctgtagcaaa tgccgcttca tcttttgcgt
    10201 ttgctcgtcc agcgtgatgg gctggctgac atgttgtgta agcactggac tcatagctgc
    10261 tccttagcca ccagcggtac atctgtatcg tcgttgatca gcgtcgcgcg ttgtttgcac
    10321 tccgggcaga cgctcgcttg cgcccgcaac atttcgcggt tttgtggcgc attttgttgc
    10381 tgtgccaaca attcagcagc cagtgcgacg gttttttgcg gggcaaacgg gcgttcgcaa
    10441 cggctgcaac gttgtagatg gaacgtcgcg cgggtataga gatcggcttt attggtgacg
    10501 gtcagttcaa agttattggt aagctggatg gctctggtcg ggcacacttc ttcacaacgt
    10561 ccgcagtaaa tacaacgccc cagatagagc tgccaggtgc gcgaattttg ctggtcgtcg
    10621 gtctggatag tcagcgcatt tgtcggacaa gcacaggcgc aggcaccaca ggcaatacat
    10681 tggctgggca tcaggtccgg tttcccgcga aagcctgggc tgacctccag tggcgcgaag
    10741 ggatatttca ccgtcgcggt tccggcgcgc ataatagttt tcagtaactt cagcatcttc
    10801 tgtccttact tcagcggcga acggttacga tcaatgccgt agcgttcgat ctctttatac
    10861 ggcacggttt ttgactggcg cttgcgcaca tcaaccagcg tcacacggtc ggtacaggag
    10921 tagcagggat caaggctacc gataatcagc ggtgcgtcag aaacggtatt gccgcgcagc
    10981 atgtaacgca acaccggcca gttggcgtag gtggcggcac ggcagcgcca gcggaacaat
    11041 ttttggttat caccgagcat gctccagtgc acgtcttcac cgcgtggcgc ttcaacaaag
    11101 ccgagcgcga atgcgtgagg tttatagcta aagccttcgg tcagcagtgg ggtatccggc
    11161 atgttgtcga gagcaaattc cagcattgcc agcgaatcaa acgtctcttt gacacggacc
    11221 atcacgcggg agaaaacatc gccgccggtg aaggtaaaca gtgtttttgg aatattgccg
    11281 tagtcggcgt aggggtgatc aaagcgcaaa tcacgggcaa aaccactgcc gcggatcagc
    11341 ggccctacag ggctataatc acgggcgatt tgtcggtcga gaatgccaat gccctgagtg
    11401 cgttgttcca tattcggcgt ggcaagcagc atttctacca gctccgacac gtcggcgcgc
    11461 atctcgcgca ccagtttcag cgtttgcaga cgttgctctt tgagaatatc gcggcgaaca
    11521 ccaccaatca gattcagacc gtaggtttta cgcgacccgg tcagcaattc cgccattgtc
    11581 atcgactttt cccgcacgcg gaaaaattgc ataaagccgg tatcaaatcc aacaaaatgg
    11641 caggagaggc caaggttgag caaatgactg tgtagccgtt cgacttccag cagaatcgag
    11701 cgaatggtat gcgctcgttg cggcacctca atccccagtg cattttcaac cgagttggta
    11761 taggccacac tgtgggcaaa accgcaaatc ccacacacgc ggtccgataa gaaggtcact
    11821 tcgttgtagc ccatccgcgt ttctgccagt ttctccatgc cgcgatggac ataaaacaga
    11881 cggtaatcag catcgacaat ttgctcgcca tccacaaaca agcggaagtg acccggttca
    11941 tcggaggtga tatgcagcgg gccgacaggg atcacccgcg caccgctgtt gccctcattg
    12001 ataaacggat acgtttcgga atcagtcgtc ggttcagggc gcagtcgata atccatcgca
    12061 tctttgcgca gcggatgcat atcttccggc cagtcatcgg gcaacaccag gcgacgctga
    12121 tccggcaggc caaccggaat cagcccgtac atatcacgaa tttctcgctc gccccagacc
    12181 gcggcaggga cgcgcggtgt gactgacgga aactcccgac tgtcggcatc gaccagcgcc
    12241 ttcaccacaa tccagcattt ttcggcccct tccattgaaa gggcataata aaccgcgtaa
    12301 tgaccgttaa gtgtccgctc gtcgttgcca aacaggaccg gaagccagcc atcatgttga
    12361 taataaagat actgtacaac gtcaggcagc agattgattt tcaccgtaat ggtgacctgt
    12421 tcaggcgttt gtcgctcttc atccagcacc gcgccgggga actgcgtttt cagggcggcg
    12481 agaatcgctt cgccacgatt tgacgatgaa ttaacgttca ctgtgtttct ccgaaacaga
    12541 tgcggtgcct gaaggcaaaa aatcatgcca ggtgctacgt tgtgcaggca ggtcgtgcgt
    12601 ccctgagagg actatagtgg aagcgcccgc caggatcctg atgacaggtt gtggaatatg
    12661 cgttcccatc gccagcatca tgaccagcag aatcaccatt ggcgaggtgg tcaaccagcc
    12721 gagatcaccc cggttaacgg cctgcggcgg tttcgccatt aacacccgcg cagccatccg
    12781 taccaggccc gccagcacca gcgttaacag caataacagc aggacgataa tcagcaggtg
    12841 attacgtgcc agtccggcgg taacggtcat aaattcgcta agaaaaatgt tgaagggcgg
    12901 catccctgcc agcgccagcg caccgccgcc aaacagcacg gcggtaaatg gcatgatttt
    12961 gagcatccca cagacgacgt tgagatcgcg cgtgccgtac ttgagcagta cattgccgga
    13021 accgcagaac agcagcgttt ttgccagact gtggtttaag gtgtgcagca gcgcggcaaa
    13081 aattcccagc ggcccgccaa tgcctagcgc caccgcgacc agtcccatgt tctccacgct
    13141 ggagtacgcc agcagacgct taatgtcccg ctgtaccaga atgaaaaatg ccgccacggc
    13201 aaccgacaac atgccgaaga tgagcaacaa ccggttgggg aaatcgctgc cgatggcctg
    13261 gcaaataatg atgtaatagc gaatcagcac caacagcgcg cagttcagca atacggcgga
    13321 gagtagggcg ctgaccggac tcggcgcttc actgtgagca tccggcagcc aggcgtgcat
    13381 gggaaatagc ccggttttgg taccaaagcc aattagcaca aacacaaagg ccaacagcat
    13441 taatgttgga tcaagcaagg acgattgctt aagaacctcg ctccagaata tcgccatttc
    13501 tgcctgcggc ataacgctgg cggcgttggc gtataccagc acggtaccga acagaccaaa
    13561 agcgacacca acagtacaaa taatgatgta cttccatgct gcttccagcg atgaacgctg
    13621 accgtaaatg cctaccagaa acgccgagct taaggtggtg gcttcgatcg ccgcccacat
    13681 cacaatcagg ttattgctgg taacaaccag cagcatggtg aacaaaaaca gatggaagaa
    13741 accgtagtaa tcgcacagcg taacgggtga aagctcgccg tgctcaactt catgacgcat
    13801 gtagccaatc gagtaaacac cggtgagaaa gccaatcaca ccaagaatgg cgaggaacaa
    13861 accgcccaga ccatcaatat gaagccacag tcccgcagcg aatatttctc ctgcatcagc
    13921 ggcagtttgg accacccaga gtgccagaat cagcagcagt gtgatcccta agctatgtaa
    13981 tactgtcacc gtgcgagtcg cagagtgccc tcgtttccgg caggcaaaac agagcagcga
    14041 aaaaagcagc ggagtgagca ggagtaaagc gaacatcaca gaataactca tctcattatc
    14101 ccttcagcgc ggtcaagttg ttcacgtcca gcgtgccgtg ggtacgccat atttttcttg
    14161 ccagtaacac catcacaatg acggcgaaga tggcgtcggt agcgatacca atttccacca
    14221 gttccggtgc acgccaggct agaagcgcca gcaccagatg ggagccgttt tccatcaggc
    14281 agtaaccaaa aatttgccgc aggatattgc gctggctaac aatgcacagc aggccaagca
    14341 gaaaatgacc taacgccacc gccagcgctg gtttcagccc ggtagccatc ggtagcttca
    14401 cgggctgaac gacaaatgcg caaagcagga caattaacgc cgcgagcagt gccatcattg
    14461 ccggaccgaa taacgctttt tccgggatgt tctggggaat atttcgtgca gcgtaagtca
    14521 tgattaacgg taccagcagc actttggtga taaaggcgct ggcggaccag atcagcagtt
    14581 gctctgcggc gaacaggcac gagagggtgg cgaaaataga caccagcacc agcgactggc
    14641 aggcgtaaaa tccgcatgac aggcgatagc ttttgacgct aatcacaaac agcgatgtca
    14701 gcatcatcag tcccgccaga ttatttacga tcatagaacc ggtcattcat catctcctta
    14761 ctgcaaccag gtcgcggcga ttacgctgga aatcagcgac atcacaatca gtacaatcag
    14821 caccaggcgc attgaccctg gcagcggtgc ggcatcggcg acggcctcgc tcggtttgcc
    14881 ggggacgacg cgaccaaacc agcgaataaa ccaggcgaaa ctggcgaccg attcaatcat
    14941 cagcagaatc atggcgggca gcaggatcca gtactccact gacaacgcaa aaccggcagc
    15001 aaacagcggg aatttactga agaagccgtt gaacggcggt acgccggtaa tcgccagcgc
    15061 tgccacgcag aaacccacgc ctggcaacgg cagggtgtgc aatacgccac gcagacgcgg
    15121 caacaagcgc gtgccgcagc tgtaactcag cgcacctgct acaaggaaaa acaggctttt
    15181 agcgaacgcg tggttgacga tgtaggcgat gctgccctcc agcgccagcc gcgagccgaa
    15241 gatggagagc gacaagccga agaacatcca gccaagttga gtgatggtcg accaggccag
    15301 caaccgcttc atatcctgct gtggcaaata catcagaaat ccataaagaa tggtgaccag
    15361 ggccatgacc atgccaacgc cgccaatcac atgcgggata ttgccgccgt cgataatggc
    15421 gcgggcaaaa atgtaaacgc ccactttcac catcgatgcg gcgtggagat aggcgctgat
    15481 cggtgttggc gcttccattg cgtccggtag ccacgcttgc atcggtagct gggccgattt
    15541 cccccacgcg gcaaacagga tgccgccata aaccagataa cgtgcgtcgc cgtgtaactc
    15601 gctcatcgcg ctaagcgcaa acgttccggt ctgcaaaaac agcgtggcgg cggcaagata
    15661 caaccccaac gaaccgatat gggtgataag caacgctttt agtgctgaac gctgcgcttt
    15721 atcgctctga taataactga tcaacaccca ggagcagccg cccgtaattt caaaaaacaa
    15781 caactgaccg agcagcgtcg aggagagtac cagtcccgcc atcgcgccga taaacaccag
    15841 caaaaatgcg taataacgat tcgtgccgtt atgcgggtgt tcgcgatttt tatccgtcag
    15901 ataacccgtg gagtagatcg tgaccagcaa accgagaaac accaccacaa acagaatcag
    15961 cgtacacacg cgatcaatga ccaggccaaa caatgccaca tcccccacgt tgaccaacgt
    16021 caacgtgacg gcaactttat cggcctgata aaacgcggag atcagcgaca acatgcacag
    16081 cgtggtcagc gcggcgaaca aaacccccca ttcggcggcc cgacgttgtg gcgaaaacga
    16141 aacgaccagt gcgccgataa aaggcagcaa taacgtcgtc agagcaagat tttccatatt
    16201 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
    16261 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
    16321 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    16381 gcaggcaggc gagagaaagt tcttgcgcgc acccaaaggg cagaaacagg gccacaaaca
    16441 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgccagcgcc agcccggcac
    16501 cggaatattc tgtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    16561 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    16621 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    16681 ctgctatcag cgccaatacc agcagtgaga gaatgagcat tggttcgacc agaatgccga
    16741 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agcccggaaa
    16801 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    16861 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    16921 tgattaatac ccacggcatc aggcggaaca tcagacctga agatataggc gcaacttcct
    16981 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    17041 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagcggggtt agcatcagca
    17101 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    17161 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    17221 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    17281 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    17341 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    17401 agacgaaata gcgcagagaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    17461 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    17521 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    17581 aacagcaaca gtaatgaggg ggtgacctgt gtatgaaacg tgccgggtac aagtgctatt
    17641 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatatg cattatcttc
    17701 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    17761 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtact gcgcggcgca
    17821 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    17881 gccagcatca ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    17941 agtgagaaca gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaagg
    18001 gctgatatcg cgaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    18061 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc gcccgcgccg
    18121 agaaatagca gccctttgaa cagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    18181 ccaaccacgg tgagcagcgg gtcgtgtagt gacagaccga ccatcgccac accgactgcc
    18241 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    18301 tccgccagcg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    18361 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    18421 ccgaaaatac cgattttgac cattacgcca gacatcaacg ccgaagcgtg cgatggtgcg
    18481 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    18541 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    18601 aaactggcaa aatctaggct gccgctttcg cgccccatca gcaagaaggc gatcataatc
    18661 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgag cgacgtttta
    18721 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    18781 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    18841 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    18901 agcgaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    18961 attcgcaggg ttaaatcggc aaagtcaaaa ggggtggcaa aacgggcaac taaagttacg
    19021 ccttcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    19081 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    19141 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    19201 cctgagtgag ggaaagggga tcctccctgc cattggcaaa ccagcttgcg gcaaggcgct
    19261 gtttttcttt catctgacgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    19321 ggcaagcgcg aacgcaggca ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    19381 cgacggtctg gacacccggt tcccagcgca gcaaagcatg ttgtgttggc gcgttttctt
    19441 cgccgtcttt gagtgagcct tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    19501 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    19561 tacagaggct ttcgttgagt tggatcgcat catccctctg ggagatggca ttgaccgggc
    19621 agacctgcac gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    19681 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    19741 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    19801 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcataatg cataaaggat
    19861 gccagtatga ttaatttaat atattcaata agttatgaat taactgaatt ctgaacggta
    19921 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    19981 aagtgagaat gtgatcttga tcacgataag tgaggtgtgc tgaccgcaag cgcagccagc
    20041 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    20101 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    20161 agctgatgcg atgaatacca tcgtaggttt tgcccatgaa ggacttttcc ccccagacgc
    20221 cgaattgctc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    20281 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    20341 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    20401 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    20461 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    20521 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    20581 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    20641 gtccgcagat gcggggctgt gggcagtaat ctgaatatgc aactgcgcag cccgttcatt
    20701 ttcagcaggt tgtgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    20761 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    20821 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    20881 ttcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    20941 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    21001 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    21061 caacgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    21121 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    21181 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    21241 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    21301 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    21361 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    21421 tgtggtgatg atgacgctgg agctggctga cgggcgcatt ccggtgattg ccgggaccgg
    21481 tgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    21541 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    21601 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    21661 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    21721 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    21781 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    21841 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    21901 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    21961 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    22021 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    22081 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    22141 tgatggctta ctctgttcat aagtcgcgcc tggcaacggt tgcgggtgtt tcgcttgttt
    22201 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    22261 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    22321 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    22381 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    22441 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    22501 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    22561 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    22621 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgctga
    22681 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    22741 agatgatgaa cgttatttcc gctggtctgg ataaatctgc cactgacgcc gcgaacgctg
    22801 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    22861 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    22921 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    22981 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    23041 ctggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    23101 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    23161 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    23221 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    23281 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    23341 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    23401 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    23461 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    23521 tcgcaaaggt atggtgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    23581 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    23641 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gctggctctc tggtgaaacg
    23701 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    23761 cgacgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtaag
    23821 caaagagaac ctggcacgca tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    23881 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    23941 aggtgaagtg gtactgggcg atgagttctc cccggacggt agccgcctgt gggacaaaga
    24001 aacgctggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaggc
    24061 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    24121 ttgccgtgct gctggcacgg caagacaacc attctcgtaa gatgtgcatt gaacgctcat
    24181 tcctccgcca tttcatcccg ttttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    24241 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    24301 aatgttgaag acaggcgcaa tagctccggc ggtccgtcaa tgggcggtcc cggtttccgt
    24361 ctgccgagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    24421 ggcgttgatt taaccgggtt gatgaccggg cagccggttt ctcaacagca atcaacgcgg
    24481 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    24541 gaagacacct ggggacaaca gttcgagaag atgggtaaga cctatcagca accgaagctg
    24601 gtcatgtacc gtggaatgac gcgtaccgga tgcggggcgg gccagtccat aatggggccg
    24661 ttctattgcc cggcggatgg tacggtttat atcgatctct ccttctatga tgacatgaaa
    24721 gacaaacttg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    24781 catcatgtgc agaaactgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    24841 acgcaggcgg aagtgaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    24901 ggtgtctggg ggcatagcat gcagcagcaa ggcgttctgg aaaccggcga tctggaagag
    24961 gcgctgaacg cggcgcaggc catcggcgat gaccgtttac aacagcaaag tcaggggcga
    25021 gtagtaccag acagtttcac tcatggcact tctcagcaac gctacagctg gtttaaacgt
    25081 ggtttcgaca gcggcgatcc ggcacaatgc aatacttttg gtaaaagcat ttaacttccg
    25141 gggcagggat ggctgaactg actgcgcttc acacattaac agcgcaaatg aaacgtgaag
    25201 ggatccgccg cttgctggtg ttgagcgggg aagagggttg gtgttttgat catgcgctta
    25261 agttacgtga tgccttacct ggcgactggc tgtggatttc gccgcagcca gatgctgaaa
    25321 accactgttc tccctcggca ctacaaactt tacttgggcg cgagttccgg catgcggtat
    25381 tcgacgcccg ccacggcttt gatgccgctg cctttgccgc acttagcgga acgttgaaag
    25441 cgggaagctg gctggttttg ttactccctg tatgggaaga gtgggaaaac caacctgatg
    25501 ccgactcgct gcgctggagt gattgccctg accctattgc gacgccgcat tttgtccagc
    25561 atttcaaacg cgtacttacg gcggataacg acgctatctt ctggcggcaa aaccagccgt
    25621 tctcgttggc gcattttact ccccgtactg actggcaccc cgcgaccagc gcaccacaac
    25681 cagaacaaca gcaaatctta cagcagctac tgaccatgcc gtcgggcgtg gcagcggtaa
    25741 ctgctgcgcg tgggcgcggt aaatcggcgc tggcagggca actcatttct cgtattgcgg
    25801 gtagtgcgat tatcactgcg cccgcaaaag cggcaacgga tgtactggca caatttgcgg
    25861 gcgagaagtt tcgctttatt gcgcctgatg ccttgttagc cagcgatgag caagccgact
    25921 ggctggtggt cgatgaagcc gcagccatac ctgcgccgtt gttgcatcaa ctggtatcgc
    25981 gttttcctcg aacgttgtta accactacgg tgcagggcta cgaaggtacc ggacgtggtt
    26041 ttttgctgaa attttgcgct cgctttccgc atttacaccg ttttgaactg caacagccga
    26101 tccgctgggc acagggatgc ccgctggaaa aaatggttag tgaggcactg gtttttaacg
    26161 atgaaaactt cacccataca ccacaaggca atatcgtcat ttccgcattt gaacaaacgt
    26221 tatggcgaag cgagccagaa acgccgttaa aggtttatca gttattgtct ggtgcgcact
    26281 accggacttc gccgctggat ttacgccgca tgatggatgc accagggcaa cattttttac
    26341 aggcggctgg cgaaaacgag attgccggag cgctgtggct ggtggatgag gggggattat
    26401 ctcaagaact cagtcaggcg gtatgggcag gttttcgtcg cccgcggggt aatctggtgg
    26461 cccagtcgct ggcggcgcac ggcagcaatc cactggcggc gacattgcgt ggacggcggg
    26521 tcagccggat agcagttcat ccggcgcgtc agcgcgaagg cgttgggcaa cagctcattg
    26581 ccagcgcttt gcaatatagg cctggcctcg actatctttc ggtgagtttt ggttacaccg
    26641 gggagttatg gcgtttctgg caacgctgcg gttttgtgct ggtgcgaatg ggtaatcatc
    26701 gtgaagccag cagcggttgc tatacggcga tggcgctgtt accgatgagt gatgcgggta
    26761 aacagctggc tgaacgtgag cattaccgtt tacgtcgcga tgcgcaagct ctcgcgcagt
    26821 ggaatggcga aacactccct gttgatccac taaacaatgc cgtcctttct gacgacgact
    26881 ggcttgaact ggccggtttt gctttcgctc atcgtccgct attaacatcg ttaggttgct
    26941 tattgcgtct gctacaaacc agtgaactgg cattaccggc gctgcgtggg cgtttacaga
    27001 aaaacgtcag cgacgcgcag ttatgtacca cacttaaact ttcaggccgc aagatgttac
    27061 tggtccgtca gcgggaagag gccgcacagg cgctgttcgc acttaatgat gttcgcactg
    27121 agcgtctgcg cgatcgcata acgcaatggc aattttttca ctgactcctt caattttctc
    27181 ccatggtcat tgtgagtcag cggcgtaaaa ttaatgacat cagttaagga gttcgccatg
    27241 aaacatgacc attttgttgt tcaaagcccg gataaacctg cgcaacagtt gttactgctt
    27301 ttccacggtg tcggggataa cccggtggca atgggggaaa taggtagctg gtttgcaccg
    27361 ttgttcccgg atgcgctggt ggtgagtgtc ggtggcgcgg agccgagcgg taatccggcg
    27421 gggcgtcagt ggttttcggt gcagggtatt acggaagata atcgccaggc gcgtgtggat
    27481 gccatcatgc cgacgtttat tgaaacggtg cgctactggc agaaacagag cggagtaggg
    27541 gctaacgcca cggcgctaat cggtttctct caaggcgcga ttatggcgct ggagagcatt
    27601 aaagccgaac cgggccttgc ttcacgcgtc attgctttta acggacgcta tgcgagcctg
    27661 ccggaaaccg cgtctacagc cacgacaatt cacttgattc atggcggtga agatccggtt
    27721 atcgatctgg cgcatgcagt tgcggcgcag gaagcgttaa tcagtgccgg tggtgacgtg
    27781 acgctggata tcgtggagga tttaggccat gcgatcgaca atcgcagtat gcaatttgcc
    27841 ctggatcact tgcgttatac cattccgaaa cattatttcg acgaagcgct aagcggcggt
    27901 aagcctggcg atgatgatgt gattgaaatg atgtaataat agccgggtag gcctgataag
    27961 accgtatcag gcatcatggc cggatgcggc gttaacgcct tatccggcca cttagcttac
    28021 ttcttcggtt gatccttttt cggccagtcg tcgtcatcgt cccacttatc gttgaaatca
    28081 cgatgcgggg gaagctccgg cttgttggcg agaaatttct tatggtcgac gcgcttgaga
    28141 tctttaatca cgttcagcag gacgcctacc aaaaagacaa tcaccagaat ccaccaatat
    28201 ttagccagcc agtccatgct tatttcctct tgcagaacca ctcatcacgc gacgagctgt
    28261 tccatgatac gttgatacat acgggcaagt agctgcaggt cggcagcgtt cacacattca
    28321 ttaattttat gaatagtggc attgaccggc ccgagttcca ccacctgcgc ccccatgagg
    28381 gcaataaagc gcccgtcgga cgttccgcct gtggtcagta gctgcggttt aatttcatta
    28441 tagtgctcaa ccgcgttaac gaccgcatcc accagtttac cgcgcgcggt caaaaatggc
    28501 tgcccggaaa gccaccaatc caccgtatag cgcagttgat gtttttcaag cagggcaagc
    28561 acctgcgctt tgatcatctc atcagtcagt tcggtgctga agcggaagtt aaactgcaca
    28621 aacagttcac ccggaataac gttgttactg cccgttcccg cctgaatatt ggcaatctgc
    28681 atactggtcg ccgggaagaa ttcattgccc tgatcccact caatagccac taattcatta
    28741 aggaaaggtg ctgcgcgatg taccggattg tcagccagat gtgggtaggc aacatgcccc
    28801 tgaacgccat gaatggttag gttgcaggtt aacgatccac gacgaccatt tttcaccaca
    28861 tcacccacca cttcgatact cgacggttcg ccgaccaggc agtaatcgag acgctcatta
    28921 cgtgccatta acgcttcgac ggcttttacc gtaccattgt gggcactggc ttcttcatca
    28981 gaggtgatca gaaatgccag tcgccctgta tggttgggat gttgtgcgac aaaacgttct
    29041 gcagctacca ccatcgccgc cagcgagcct ttcatatctg ccgcaccgcg cccgaataac
    29101 atgccgtcac gaatggttgg ttcaaacggc ggattgatcc aacgatcggc gtcgccaggc
    29161 ggcaccacgt cggtatgccc ggcaaaggcc aatgtttcac cttgcccacg ccatgcccaa
    29221 aaattctgcg tatcggcaaa gtccatgcgt tcaacggtaa aaccgatcgc ctgcaaacgt
    29281 tcaatcaaca aagcctggca tcctgcgtca tcaggactca gggaagggcg gcgaataagc
    29341 tgttgtgtca gctcaataac cgggcacgac atagactaca cctcatggaa aaatttctga
    29401 taactggaat cactgaaacc cagcagcata ggcttaccag gcgcgcagag caatggacgt
    29461 ttgataattg caggcatttc agtcattaat gccgccgcag aggccgcatc ggtgattata
    29521 ttgcgggtgg tttcgtccag tttacgccag gttgtaccac gggtgttgag taacgcttcc
    29581 cagcctaatt cgttgataaa accgttcaat aattcgctgt ccagcccatc gacgcggtaa
    29641 tcatgaaaac gatagtcgat gttattggct tccagccaac ggcgagcctt tttaatggtg
    29701 tcacaatttt tgatgccgta aagtgtaacc ataatgaatc ctttttcgat ttatgagaat
    29761 aaatactcat ttaagagtga aaacattttg cctgcaaatg attatcagga aatataacat
    29821 tatcgaagta ttcaaattaa cagctattaa ataatat
//
LOCUS       DAZFBR010000056        29657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFBR010000056 DAZFBR010000000
VERSION     DAZFBR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.056"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..412
                     /locus_tag="ACHJZ2_002662"
     CDS             <1..412
                     /locus_tag="ACHJZ2_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4568746.1"
                     /translation="DVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKN
                     SLQMRIDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSG
                     HGTVNMRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(455..1315)
                     /gene="rsmI"
                     /locus_tag="ACHJZ2_002663"
     CDS             complement(455..1315)
                     /gene="rsmI"
                     /locus_tag="ACHJZ2_002663"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO4568747.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1380..3416
                     /gene="lpoA"
                     /locus_tag="ACHJZ2_002664"
     CDS             1380..3416
                     /gene="lpoA"
                     /locus_tag="ACHJZ2_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO4568748.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3374..3769
                     /gene="yraN"
                     /locus_tag="ACHJZ2_002665"
     CDS             3374..3769
                     /gene="yraN"
                     /locus_tag="ACHJZ2_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO4568749.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            3789..4379
                     /gene="diaA"
                     /locus_tag="ACHJZ2_002666"
     CDS             3789..4379
                     /gene="diaA"
                     /locus_tag="ACHJZ2_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO4568750.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4389..4964
                     /gene="dolP"
                     /locus_tag="ACHJZ2_002667"
     CDS             4389..4964
                     /gene="dolP"
                     /locus_tag="ACHJZ2_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO4568751.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5078..6118)
                     /gene="yraQ"
                     /locus_tag="ACHJZ2_002668"
     CDS             complement(5078..6118)
                     /gene="yraQ"
                     /locus_tag="ACHJZ2_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO4568752.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6191..6826)
                     /gene="yraR"
                     /locus_tag="ACHJZ2_002669"
     CDS             complement(6191..6826)
                     /gene="yraR"
                     /locus_tag="ACHJZ2_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO4568753.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6954..7472
                     /gene="yhbO"
                     /locus_tag="ACHJZ2_002670"
     CDS             6954..7472
                     /gene="yhbO"
                     /locus_tag="ACHJZ2_002670"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO4568754.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNRETLRLLGA"
     gene            complement(7452..7895)
                     /gene="yhbP"
                     /locus_tag="ACHJZ2_002671"
     CDS             complement(7452..7895)
                     /gene="yhbP"
                     /locus_tag="ACHJZ2_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO4568755.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            7946..8248
                     /gene="yhbQ"
                     /locus_tag="ACHJZ2_002672"
     CDS             7946..8248
                     /gene="yhbQ"
                     /locus_tag="ACHJZ2_002672"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO4568756.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(8235..8738)
                     /gene="yhbS"
                     /locus_tag="ACHJZ2_002673"
     CDS             complement(8235..8738)
                     /gene="yhbS"
                     /locus_tag="ACHJZ2_002673"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4568757.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8732..9256)
                     /gene="ubiT"
                     /locus_tag="ACHJZ2_002674"
     CDS             complement(8732..9256)
                     /gene="ubiT"
                     /locus_tag="ACHJZ2_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO4568758.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9465..10460
                     /gene="ubiU"
                     /locus_tag="ACHJZ2_002675"
     CDS             9465..10460
                     /gene="ubiU"
                     /locus_tag="ACHJZ2_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO4568759.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10469..11347
                     /gene="ubiV"
                     /locus_tag="ACHJZ2_002676"
     CDS             10469..11347
                     /gene="ubiV"
                     /locus_tag="ACHJZ2_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO4568760.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAILDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11428..12435
                     /gene="yhbW"
                     /locus_tag="ACHJZ2_002677"
     CDS             11428..12435
                     /gene="yhbW"
                     /locus_tag="ACHJZ2_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFO4568761.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12552..13796)
                     /gene="mtr"
                     /locus_tag="ACHJZ2_002678"
     CDS             complement(12552..13796)
                     /gene="mtr"
                     /locus_tag="ACHJZ2_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO4568762.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13950..15839)
                     /gene="deaD"
                     /locus_tag="ACHJZ2_002679"
     CDS             complement(13950..15839)
                     /gene="deaD"
                     /locus_tag="ACHJZ2_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO4568763.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15832..15912)
                     /gene="yrbN"
                     /locus_tag="ACHJZ2_002680"
     CDS             complement(15832..15912)
                     /gene="yrbN"
                     /locus_tag="ACHJZ2_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO4568764.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16019..16903)
                     /gene="nlpI"
                     /locus_tag="ACHJZ2_002681"
     CDS             complement(16019..16903)
                     /gene="nlpI"
                     /locus_tag="ACHJZ2_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO4568765.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17012..19147)
                     /gene="pnp"
                     /locus_tag="ACHJZ2_002682"
     CDS             complement(17012..19147)
                     /gene="pnp"
                     /locus_tag="ACHJZ2_002682"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO4568766.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19394..19663)
                     /gene="rpsO"
                     /locus_tag="ACHJZ2_002683"
     CDS             complement(19394..19663)
                     /gene="rpsO"
                     /locus_tag="ACHJZ2_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO4568767.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR"
     gene            complement(19812..20756)
                     /gene="truB"
                     /locus_tag="ACHJZ2_002684"
     CDS             complement(19812..20756)
                     /gene="truB"
                     /locus_tag="ACHJZ2_002684"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO4568768.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20756..21157)
                     /gene="rbfA"
                     /locus_tag="ACHJZ2_002685"
     CDS             complement(20756..21157)
                     /gene="rbfA"
                     /locus_tag="ACHJZ2_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO4568769.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21322..23994)
                     /gene="infB"
                     /locus_tag="ACHJZ2_002686"
     CDS             complement(21322..23994)
                     /gene="infB"
                     /locus_tag="ACHJZ2_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO4568770.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24019..25506)
                     /gene="nusA"
                     /locus_tag="ACHJZ2_002687"
     CDS             complement(24019..25506)
                     /gene="nusA"
                     /locus_tag="ACHJZ2_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO4568771.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25534..25986)
                     /gene="rimP"
                     /locus_tag="ACHJZ2_002688"
     CDS             complement(25534..25986)
                     /gene="rimP"
                     /locus_tag="ACHJZ2_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO4568772.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26193..26269)
                     /locus_tag="ACHJZ2_002689"
     tRNA            complement(26193..26269)
                     /locus_tag="ACHJZ2_002689"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26233..26235),aa:Met,seq:cat)
     gene            26617..27960
                     /gene="argG"
                     /locus_tag="ACHJZ2_002690"
     CDS             26617..27960
                     /gene="argG"
                     /locus_tag="ACHJZ2_002690"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO4568773.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(27968..29593)
                     /gene="yhbX"
                     /locus_tag="ACHJZ2_002691"
     CDS             complement(27968..29593)
                     /gene="yhbX"
                     /locus_tag="ACHJZ2_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4568774.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIVHYKGDS
                     LVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT         7165 a         8089 c         7412 g         6991 t
ORIGIN      
        1 cgatgttatt aaggttaatt ttggattcat caatggtcag aagtttacca cccgcaatgc
       61 catgccagac ggttttactc cagtagactt tgatatcact tatgactgtg gtgatacttc
      121 aaagattaaa aactcgttgc aaatgcgcat cgacggtaca actggggtag tagaccagta
      181 caacctggtc gccagacgaa gaagttcaga caatgcgccc gatgtcggta ttcgtattga
      241 aaatctcggc ggcggagttg caaatattcc ttttcagaac ggtatccttc ccgttgatcc
      301 ttccgggcat ggcaccgtca atatgcgcgc ctggccagtt aatctggtcg gtggtgagct
      361 ggaaacagga aaatttcagg gcacagccac cattaccgtc atcgtgcggt aaaagaagac
      421 gttgcgtttt gattaacgca acgcttcggc actcttaccc ctgctgctcc agcgcatact
      481 tatacagcgc atttttcttc acgccgtgaa tttctgcggc cagcgccgcc gcttttttca
      541 gcggcagttc tgcctgtagc agcgccagcg tgcgcagggc atcggcgggt aagtcttctt
      601 cctgtgcttt atgaccttcg acaatcagca ccatttcgcc tttgcgacgg ttttcatctt
      661 cctttaccca cgccagcagc tcgccaacgg gcgcgccgtg aatggtttcc caggttttgg
      721 tcagctcacg cgccagaacc acgtagcggg attcgcctaa taccgcaacg atatcttcca
      781 ggctatctaa cagacggtgg gtagattcat aaaaaatcag cgtgcgcggc tccgcttcaa
      841 tggcttttag cgcatcacgg cggccttttg atttggcagg taaaaagcct tcgtaacaga
      901 aacggtcaga gggtaaaccc gcggcgctta acgcagtgat agcagcacac ggtccgggta
      961 gcggcaccac acgaatcccc gcttcacggc aggtacgcac cagatggtag ccaggatcgt
     1021 taattagcgg cgttccggca tcggaaacca gcgcaatgtt ttgcccctct tgcagcttcg
     1081 ccagcagcgt ttcggctttt tgttgttcgt tatggtcgtg cagcgcaaac aaccgggcat
     1141 taatcccaaa atgttgcagc aataaaccgg tgtgacgagt atcctcggcg gcaatcagat
     1201 caacggcctg taatacctct aacgcacgct gggtgatatc cgccagattg ccgattggcg
     1261 tcggtacaat gtaaagctgg ccctgagaat tatccgccga ttggtgttgt ttcattgtgt
     1321 cgtccgtatt gccgatttaa tattgagcat tgcgtaaaaa aaatatcact ggatacatta
     1381 tggtaccctc aacattttct cgtttgaaag ccgcgcgttg tctgcctgtt gttctggcag
     1441 ccctgatttt cgtcggttgt ggcacccata ctcccgatca gtccactgct tatatgcagg
     1501 gcacggcgca ggctgattct gccttttatc ttcagcagat gcagcaaagc tctgatgata
     1561 ccaggatcaa ctggcaatta ctcgccattc gtgcactggt gaaagaaggt aaaaccgggc
     1621 aggcggttga gttgtttaac caactaccgc aagaactgaa cgattctcag cgtcgcgaga
     1681 aaacactgct ggcggtagag attaaactgg cgcagaaaga ttttgctggc gcgcaaaact
     1741 tgctggcgaa aattacaccc gccgatttag aacaaaacca gcaagcccgt tactggcagg
     1801 caaaaatcga tgccagccag gggcgtcctt ccattgattt actgcgcgcg ttaattgctc
     1861 aggaaccgct gcttggcgcg aaagaaaaac agcagaatat tgatgccacc tggcaggcgc
     1921 tctcctccat gactcaggaa caggcgaata cgctggtgat caacgccgac gaaaatattc
     1981 tgcaaggctg gctggatctg cagcgcgtct ggtttgataa ccgtaacgat cccgacatga
     2041 tgaaagccgg gatcgccgac tggcagaaac gttatccgaa caatccgggc gcgaaaatgc
     2101 tgccaacgca gttggttaac gtaaaagcgt ttaaaccagc ctcgaccaac aaaatcgccc
     2161 tgctgttgcc actgaatggc caggcagcgg tatttggtcg cactattcag caaggctttg
     2221 aagcggcgaa aaatatcggc actcagccag tggcggctca ggtagctgcc gcacctgccg
     2281 cagacgtagc agaacaacct cagccgcaaa ctgcggatgg cgttgccagc ccggcacaag
     2341 cctcggttag cgatctgacc ggtgatcagc ctgcggccca gccggtgcct gtaagcgccc
     2401 cggcgacaag caccgcagcg gtaagcgcac ccgcaaatcc atccgcagag ctgaaaatct
     2461 acgatacctc atcacaacca cttagccaga tcttaagcca ggttcagcag gatggcgcga
     2521 gtattgtggt tggtccgttg ctgaaaaata acgttgaaga gttgctgaag agcaacactc
     2581 cgctgaacgt actagcactg aaccagccgg agaatatcga aaatcgcgtc aatatttgtt
     2641 acttcgcgct ttcaccggaa gacgaagcgc gcgatgcagc gcgtcatatt cgtgaccagg
     2701 gtaaacaagc gccgctggtg ctgatcccac gcagttcatt gggcgatcgc gtagccaatg
     2761 cgtttgcgca agagtggcag aaattgggcg gcggcaccgt tctgcaacaa aaatttggtt
     2821 ccaccagcga attacgcgcg ggtgttaacg gcggttctgg tattgcttta acgggtaccc
     2881 cgattactcc cagagcgaca accgactccg gcatgacgac caacaatcca acgctgcaaa
     2941 ccacgccaac cgatgaccag ttcaccaata atggcggtcg tgtcgatgcg gtgtacattg
     3001 tggcaacgcc gggtgaaatc gcttttatta aaccgatgat cgccatgcgt aacggtagcc
     3061 agagcggtgc aacgctgtac gccagctccc gcagtgcgca agggaccgct ggcccggatt
     3121 tccgtctgga gatggaaggc ttgcagtaca gcgaaatccc gatgctggcg ggcggtaatc
     3181 tgccgttaat gcagcaggca ctcagcgcgg tgaataacga ttattcactg gctcgcatgt
     3241 atgcgatggg cgtcgatgcc tggtcgctgg caaatcattt ctcacaaatg cgccaggttc
     3301 agggttttga aatcaacggt aataccggaa gcctgacggc taacccggat tgcgtgatta
     3361 acaggaagtt atcatggcta cagtaccaac aaggtcaggt agtccccgcc agttaaccac
     3421 caaacagacc ggcgatgcgt gggaagtaca agcgcgtcgc tggctggaag gcaaaggact
     3481 gcgatttgtc gccgctaacg tgaacgagcg tggcggcgag atcgatctga taatgcgtga
     3541 aggccggacc accgtttttg tcgaggtgcg ttaccgccgt tctgcgcttt atggcggcgc
     3601 ggcagccagt gtgacccgca gcaaacaaca caaattatta cagactgccc gcttgtggct
     3661 cgcgcgtcat aatgggagtt ttgatactgt ggattgccgg ttcgatgtgg tagccttcac
     3721 cgggaatgag gttgagtgga ttaaggatac ctttaatgac cactcataat taaggtttaa
     3781 ggattagcgt gcaagaaaga attaaagctt gcttcactga aagcattcaa actcaaattg
     3841 cggcggcaga ggcgcttccg gatgccatct cccgtgcggc catgacgctg gttcagtctc
     3901 tgctcaatgg caacaaaatc ctctgttgtg gtaatggaac ttccgctgcc aatgcacagc
     3961 attttgctgc cagcatgatc aaccgtttcg aaacggagcg gcccagctta cctgccattg
     4021 cactaaatac cgataatgtt gtcttaacgg cgattgccaa cgatcgctta catgatgaag
     4081 tgtatgcaaa acaggtgcgg gcgctggggc atgcgggaga tgtattgtta gccatttcca
     4141 cccgtggcaa cagccgcgat atcgtaaaag cagtagaagc tgccgttacg cgtgatatga
     4201 ccattgtggc attgaccggt tatgacggcg gtgaacttgc aggtttgtta gggccacagg
     4261 atgtggagat ccgcatccct tcgcatcgca gtgctcgcat tcaggaaatg catatgctga
     4321 cggtaaattg cctgtgcgat ctgatcgata acacgctttt ccctcaccag gatgattaag
     4381 gagaatacat gaaggcatta tcgccaatcg cagtccttat ttccgcgctg ctgttgcaag
     4441 gttgtgttgc cgctgccgta gtgggtactg ctgctgtggg taccaaagcc gcaactgacc
     4501 cacgcagtgt cggcacccag gtggacgatg gtaccctgga agtgcgcgtg aacagcgcat
     4561 tgtcgaaaga cgaacagatt aagaaagaag cgcgcattaa tgtaacggcc tatcagggca
     4621 aagtgctgct ggttgggcag tcaccaaacg ctgaactttc ggctcgcgcc aaacagattg
     4681 ctatgggcgt agacggtgcc aacgaagtgt ataacgagat tcgtcagggc cagccgattg
     4741 gtctgggcga agcgtctaac gatacgtgga tcaccaccaa agtgcgttcg cagctcttaa
     4801 ccagcgacct ggtgaaatcg tccaacgtga aagtgaccac cgaaaacggt gaagtgttcc
     4861 tgatggggct ggtgactgaa cgtgaagcga aagcggcggc agatattgcc agccgggtga
     4921 gcggcgtgaa gcgggtaact acggcgttta cgtttattaa atagcagccc cttgtaatgc
     4981 ctgatgcgac gcttgccgcg tcttatcagg cctacaacga tgcaaaccgt aggtcggata
     5041 aggcgtttac gccgcatccg acatgttttc cctcaaatca gaataacagc gccagcccgc
     5101 cgacaatcac gcctgacact gccaccatcg cccccgtcag ccataaggct ttcgccggga
     5161 acgctttgcg cagcataatc agtgacggca aactcaccgc cgggagcgtc atcaacaatg
     5221 ccagcgccgg agcagttccc atacctgcca gcatcatcgt ttgtacaatc ggaatttctg
     5281 ctgccgtggg aatgacaaac aagcaccctg ctaccgccat cgccaccacc cacatcaggc
     5341 tgttatcgac agcaccatcg gcatggggga ataaccagac gcgagcggca cccaacacca
     5401 gtactgcaag gatgtaaacc gggatcgtac tccagaaaag cgtccatagc gccctgcccc
     5461 agcgactaaa aaatccgccc tgtgcttccg gtatgtcaat ttcgaccggt gcttgcgttt
     5521 gcggtgtttc acgcacccat ttttgcacca gcgtcgcaat cagcaacacc atcaccagcc
     5581 cggccaccag acgaatcgcc gcaaaacccc agccgaggac aaaccccata aacaccagcg
     5641 tcgccgggtt taacaccgga ttgcccatcc agaatgccag cgcaccgccc atcgacacct
     5701 gttggcgacg cattcccgcc gcgaccggag ccgcacagca ggtacacatc atgcctggca
     5761 gcgaaaacag cgttcccagc agcgtgccgc gaaagcgcga ttgcccaagc gtacgcaaca
     5821 accagtcacg cgggatcagc acctgaatca acgaaccgag gatcaccccc agcaccgccg
     5881 ctttccatac cgcgaggaaa tagatcatcg cgtaatccaa cgccgcctgc catggattag
     5941 catccgcctg cgcaaggata gatttaccga tactgtgggt ttcggcagca gtaaacgctt
     6001 tgccgtagta aggttgccat ttcacatacc agagaccggc aatgacaacg agaaagaaaa
     6061 gcgcgggttt ccaccactga atgggcgttg ccgcctgaga tgaagactga ccagtcatag
     6121 cattccccag gaataagtta tgataattga gcgcgtgaat attacgctca ctatcaattc
     6181 ttgggaataa ttattcagct cttttgcgta attctgaaga gcttaaaatc gtcacacctt
     6241 catgctcggg ccgcatcgcc tctgccagca tgacccgcgc cacgtctctc gcatcaatgg
     6301 atttccagtt accgggtaac aaacggaaca gcggcgcaaa aagcgtttcg ttcatccgct
     6361 gtttgctacg atcgcccagt aacatcgacg ggcgagcaat ggtcaatttc ggccagttct
     6421 gggcgattaa tgcttcttcc atctcccctt tgacgcggtt atagaaaaac ggcgagtggg
     6481 cattggcacc catagcactg accaccagca tatgctgcgc gcccagtcgc cgcccggtta
     6541 atgcggtatc cactaccagc gtgtaatcgg catgaataaa cgcctctttg ctccccgctt
     6601 ctcgccgcgt ggtgccgaga caacaaaaca caatgtcgat gggatcggtg acctgcgcca
     6661 gcgcgtcagt cagttgcgga tcatggggat taaatacccc gggcatatcg cccaacggac
     6721 gtcgcgtcgg cgcggctatg gcgttaactt tcggttcgtt aatcaacatc cgcagcaggt
     6781 gaccgcccac cagccccgtt gcgcctgtaa tcagtacctg actcatcttc gctcctttac
     6841 agaattgtct gccttgcgct ccacggctca tgcaccaggc ttaatagacc atgaggtaat
     6901 tatccccgat tgtgggaaat tcgcctcatc caatgcaaca acgcggagga agcatgagta
     6961 agaaaattgc cgttttaatc actgatgaat ttgaggattc agaatttact tcacccgcag
     7021 acgagttccg taaagccgga cacgaagtga ttaccattga aaaacaagcg ggtaaaacgg
     7081 tgaaaggcaa aaaaggagaa gccagcgtga ccatcgataa atccatcgat gaagtgacgc
     7141 cagcggagtt tgatgccctg ttgctaccgg gcggtcattc accggattat ctgcgtggcg
     7201 acaaccgttt tgtcaccttt acccgtgatt ttgtgaatag cggcaaacct gttttcgcca
     7261 tctgtcacgg tccgcagctg ctgatcagcg ccgatgtgat tcgggggcgc aaactgaccg
     7321 cggttaaacc gatcattatt gatgttaaaa acgcgggcgc ggaattttac gatcaggaag
     7381 tcgtggtcga taaagatcag ctggttacca gccggacacc ggacgatctg ccagcgttta
     7441 accgcgagac gttacgcctg ctcggtgcct gagccgcgta gccaaatcat ttttttacca
     7501 aagcccagcg tgttgtcggt gaatttgatt tcatcgggcc ggatttccca caccggggcc
     7561 gacagcattc tggcaaccgg aaagcgacga ttgtacgcct tgcgcgcgag gtcgctttcc
     7621 tcaccttcca gcctgcggat ctcaccttta aactgcacgc cgcgaattaa cgctaccgtt
     7681 ttcggctgac cgtttaccgt tccggcaacc gcagcctgcg gcccactcat ctgcgcgtgg
     7741 cgcgtttttt cttccgtcaa aatgtagaag gcgactttct gcacatcaaa cagataaaag
     7801 gcattggcgc accagagttc cccttcctgc tgcacacacc aggtgacaac atgttgtttt
     7861 gccagccaac ggctgatggc ggtgagtgtt tccattgctg ttctctttta tactgtgggc
     7921 ctgaccttaa catgcgacgc agacgatgac accctggttt ctttacttga tccgtaccgc
     7981 cgacaataag ctttataccg ggatcaccac ggatgtcgaa cgccgctatc agcagcacca
     8041 aagcggcaaa ggggcgaaag cactgcgcgg gaaaggagaa ctgacgctgg cgttttctgc
     8101 gccagtgggc gatcgttcgc tggcgttacg ggcggaatat cgcgttaagc aactgaccaa
     8161 acggcaaaaa gagcgtctgg tagcggaagg cgcagggttt gcaaagctgt taagcagtct
     8221 gcaaaccccg gagattaaaa gcgattgaaa tgctcgtgat actcaaccag accagttacg
     8281 ccattcaacg cgtcatccgc taaacgatgt acctggaagg cgctttcagt gcccggccag
     8341 cggcaacgca gatcgtgatg cgccgccagt tcaaagccga aacggctgta tagcgccgga
     8401 tcgcccagcg tcaccactgc ggcatagccg aactcattaa gcgaatcgag tccttcatag
     8461 accagttggc gtgccagccc ctgtccacgg tatttttcat ctaccgccag cggtgccatg
     8521 ccgacccatt gcaggtcttc gccctgcaca tcaaccggac taaatgccac atagccaatg
     8581 acctgacctt cgtcatctgt cgccaccagc cccagcgtca gaaagccatc ttcacgcaga
     8641 tcgtgaacca gcttcgcttc cgcatcactt tcgaatgaac gacgcagcag ggcatcaata
     8701 cccggcgcat caatgggaat ttctactcga attagcatgg ttcacctacc gatgtctgtt
     8761 tggtttcagg cgcggttttc attcccgcct caacaaaatc cgccagttgc agcagcatca
     8821 tgcgtaaggc tttcggcatt tgctccagtt caatggcgtc catcaggttt ttcacataca
     8881 gccccagctc cgtatcgcct tcaatcacca gccgacgctg gaagaagagc gtatccggat
     8941 cttgtttacg cgccgcaatc atcagcagat cgctggcgtc ggcactaaaa ctcacatcag
     9001 cttgcgcgtt ctggctgacc accagtttgc cattcaccac cgaggtaaac cattgcaggt
     9061 caatatcacg cacatgaata cttaaccagc ggccttcaag aaactccagc tcaccatcat
     9121 ccagcgcctg gcggaattgc cagcttaaga cctgctcaag aacctggcgt tttagcgcaa
     9181 atggcgtcag ttttaccggt acactcaaca gagatggccc caaatgcaca atacgggaac
     9241 gcagtttatc caacacgagt tttactccct gtttcaacaa tcatcctatt ttgccatatc
     9301 agaaaaataa catagcggta taaatcaaca attccatatg aaattgctgc taccaccaat
     9361 acaactttaa ctgccttaaa tcaaaaattg tcgcagcaag gttaactaaa atcccagttc
     9421 gttaacattt ttgcgttttg atagcgcaac cttcaggaaa aattatggag ctgctctgcc
     9481 ctgccggaaa tctcccggcg cttaaggcgg ccatcgaaaa cggcgcagat gctgtttata
     9541 tcgggctaaa agatgatacc aatgcccgtc acttcgccgg ccttaacttt accgagaaaa
     9601 aattgcagga agcggtgagt tttgtccatc aacatcgccg caaacttcac atcgcgatta
     9661 acacttttgc gcatccggac ggttacgccc gttggcagcg tgccgtggat atggcggcgc
     9721 agctgggtgc cgacgcgctg atcctcgccg acctcgccat gctagagtac gccgccgagc
     9781 gttacccgca tattgagcgt catgtgtcag tgcaggcttc ggcgaccaat gaagaggcga
     9841 ttaactttta tcatcgccat tttgacgttg ctcgcgtggt gctgccgcgc gtgttgtcga
     9901 ttcatcaggt gaaacaactg gcacgggtca cacctgtccc gctggaagtc tttgctttcg
     9961 gcagcctgtg cattatgtcg gaaggtcgtt gctatctgtc gtcgtatctg acgggtgagt
    10021 cgcccaacac cgtgggcgcg tgttctccgg cccgtttcgt gcgctggcag caaacgccgc
    10081 aggggctgga atcccgcctg aacgaagtgc tgatcgaccg ttatcaggac ggcgaaaacg
    10141 caggttatcc gacgctatgt aaagggcgtt atctggtgga cggcgagcgc tatcacgcgc
    10201 tggaagaacc aaccagtctc aataccctgg aactgctgcc ggagttaatg gcggcgaata
    10261 ttgcttcggt gaaaattgaa ggccgccaac gtagcccggc gtatgtcagc caggtggcga
    10321 aagtctggcg tcaggctatc gaccgttgta aggccgatcc gcaaaacttc gtaccgcaaa
    10381 gcgcgtggat ggagacgctc gggtcgatgt ccgaaggcac gcagaccacc cttggcgcgt
    10441 atcaccgtaa atggcagtga gaagagcaat gaaatattcc ttagggccag tgctgtggta
    10501 ctggccaaaa gagacgctgg aagattttta tcagcaggcc gccaccagca gcgccgacgt
    10561 gatttatctt ggcgaagcgg tttgcagcaa gcgccgggca accaaagttg gcgactggct
    10621 ggagatggca aaatcgctcg ccggaagtgg taagcagatt gtgctctcca cgctggcgct
    10681 ggtgcaggca tcatctgaac tgggcgaact gaaacgctat gttgagaacg gtgagtttct
    10741 gattgaagcc agcgatctcg gcgtggtgaa tatgtgcgct gaacgcaaac tgccgttcgt
    10801 cgccggacac gcgctgaact gctataacgc ggtgacgctg aaaatattgc tcaaacaggg
    10861 catgatgcgc tggtgtatgc cggtggagct ttcccgcgac tggctggtga atctgcttaa
    10921 tcagtgcgat gagctgggca ttcgcaacca gtttgaagtt gaagtcctga gctacggtca
    10981 tctaccgctg gcctactccg ctcgctgctt taccgcgcgt tcggaagacc gcccgaaaga
    11041 tgagtgtgaa acctgctgca ttaagtatcc gaacgggcgc aacgtgctgt cgcaggaaaa
    11101 ccaacaagtg tttgtactca atggcattca gaccatgagc ggctacgttt acaacctcgg
    11161 taacgagctg gcatccatgc agggcctggt tgatgtggtc cgcctgtcac cgcagggtac
    11221 tgacactttc gcgatactcg acgccttccg cgctaatgaa aatggcgctg caccactgcc
    11281 gttgacggcg aacagcgact gtaacggcta ctggcggcgg ctggcaggac tggagctgca
    11341 agcctaagta aatagctcac tttgttaaca actttaacta ctctttaatg cagtattaaa
    11401 gattaatcgg taacaaagtg agctgttatg actgataaaa ccattgcgtt ttcgctactc
    11461 gatctggccc ccattcccga aggttcttca gcgcgagatg cattctccca ctctctcgat
    11521 ctcgcccgtc tggctgaaaa gcgcggctat catcgctact ggctggcaga acaccacaat
    11581 atgactggca ttgccagtgc tgccacgtcg gtattgattg gctatctggc ggcgaatacc
    11641 accacgctgc atctggggtc tggcggcgtg atgttgccta accactcacc gttggtcatt
    11701 gcagaacagt tcggcacgct caatacactc tatccggggc gaatcgattt ggggctgggt
    11761 cgtgctccgg gtagtgacca gcgaaccatg atggcgctac gtcgtcatat gagcggggat
    11821 attgataatt tcccccgcga tgtcgcggag ctggtggcct ggtttgacgc ccgcgatccc
    11881 aatccgcatg tgcgcccggt accaggctac ggcgagcaaa tccccgtgtg gttgttaggc
    11941 tccagccttt acagcgcgca actggcggcg cagcttggtc tgccgtttgc gtttgcctca
    12001 cacttcgcgc cggatatgtt gttccaggcg ctgcatcttt atcgcagcaa cttcaaaccg
    12061 tcggcacgac tggaaaaacc atacgcgatg gtgtgcatca atattatcgc tgccgacagc
    12121 aaccgcgacg ctgaatttct gtttacctca atgcagcaag cctttgtgaa gctgcgccgt
    12181 ggcgaaaccg ggcaactgcc gccgccgatt caaaatatgg atcagttctg gtcaccgtct
    12241 gagcagtatg gcgtgcaaca ggcgctgagt atgtcgctgg tgggcgataa agcgaaagtg
    12301 cgtcatggtt tgcagtcgat cctgcgcgaa accgacgccg atgagattat ggttaacggg
    12361 cagattttcg accaccaggc gcgcctgcat tcgtttgagc tggcgatgga tgttaaggaa
    12421 gagttgttgg gatagtgtgt cttaacgcgg gaagccttat ccgagctggc aacgctgtcc
    12481 cacgtaggcc tgataagcga agcgcatcag gcattgtgta ggcagcagaa atgtcggata
    12541 aggcccgctg attactgata caccggcagc aaattaaagc tcgataagat atggaccagt
    12601 gcgttgccaa cgccaaatac cagaatcagc gtaatcatcg gcttgccgcc ccagacgcgg
    12661 aatttcgggc tgccaaagcg tttacgcgat gcgcgggcca gcagtgccgg aacaattgcc
    12721 gcccagatgg tagctgctaa accagcataa ccaatggcgt acaggaatcc gttcgggaac
    12781 agcagaccac ccacaacagg aggggcaaag gtcagcaatg ccgttttcaa gcggcccaca
    12841 gccgagtcgt cgaaaccaaa cagatctgcc agatagtcaa acaaacccag cgttacgccg
    12901 aggaacgaac tcgctaccgc aaagtttgag aacacgacca gcagcagatc cagactacgg
    12961 ctgttcagta cgccgcttaa cgcctgtacc agcacatcaa tattaccgcc cttctctgca
    13021 atatcgataa actccggacg cgggatgtta cccatcgtcg ccagcaacca gatggtatac
    13081 agcgccagcg ccatcagcgt accgtacacc agacatttca cgatggtttt cggatctttg
    13141 ccgtaatact tcatcaggct tggcacgtta ccgtgataac caaacgatgc cagacagaac
    13201 ggcaaggtca tcaacagata cggtgcataa gacgcattgc tttcagcgac gttgaacaat
    13261 gtcgcaggct gcacatgccc cagcaggcta ccgaaggtga ggaagaaggt aatgactttc
    13321 gcccccaata caatcgctgt catgcgactg acggctttag tgctcaacca caccacaaac
    13381 gctaccagca atgcaaaacc aaaacccgcc gcccgtgccg ggacgtttag tgacatctct
    13441 gcgaaggtgt gatgcagaat cgaaccactg gcagaaatat aggcataggt caggatatag
    13501 agcacaaagg caatggaaat gccgttgacc acgttccagc ctttgcccag caaatctttg
    13561 gtgatggtgt caaaactcga accgattctg taattcaggt tagcttccag aatcatcaag
    13621 ccggaatgca gcatacagaa ccaggtaaag atcagcgccg ccattgacca gaaaaaccac
    13681 gccccggaca tgaccactgg cagagaaaac atccctgcgc caataatggt gccgccgata
    13741 atcaccacgc cgccaagcag cgacggtgac gtttgggtgg tggttagtgt tgccatgagg
    13801 gcttctctcc agtgaaaaat agtgcgactg cgttgttatg tattgcactg taccagtacg
    13861 cgagtacaaa agacagaaaa aaagccccga tggttcaaat cggggctgta tatattattt
    13921 tacagactgt gttcgctgtt cagcgatgat tacgcatcac caccgaaacg acgacgaccg
    13981 gtagagtcat cacgacgcgg agcgcggcct tcacgacgtt cgccgctaaa acgacgacca
    14041 tcaccacgac caccttcacg gcgttcaccg ctgaagttac gaccaccttc acgacgctcg
    14101 ccactgaaac cacgaccacc gccacgacgc tctccgccag tatgcggctg tgcatcgccc
    14161 agtaattgca tattcatcgg cttgttcaga atgcgagtac gtgtaaagtg ttgcagcact
    14221 tcacccggca tacctttcgg cagttcgatg gtggagtgag aagcaaacag cttaatgtta
    14281 ccgatgtaac ggctgctgat gtcgccttcg ttagcaatcg caccaacgat atgacgaact
    14341 tcaacaccat catcgcggcc cacttcaata cggtacaact gcatgtcacc aacatcacga
    14401 cgttcacgac gcggacgatc ttcacggtcg ccacgcggac cacggtcgtt acgatcgcgc
    14461 ggaccacggt catcacggtc acggaattca cgtttcggac gcatcggcgc atctggcgga
    14521 acaatcagag tacgttcacc ctgtgccatt ttcagcagtg cggcggccag agtttcgaga
    14581 tccagctctt caccttctgc agtcggctga attttgctca gcagtgcgcg gtattgatcc
    14641 agatcgctgc tttccagctg ctgctgtact ttagcggcga atttttccag acggcgtttg
    14701 cccagcagtt ctgcgttcgg cagttctact tccggaatag tcagcttcat agtacgttca
    14761 atgttgcgca gcagacgacg ctcgcggttc tcaacgaaca gcagcgcgcg gccagcacga
    14821 cccgcacgac cggtacgacc gatacggtga acgtaagact cagaatccat cgggatatcg
    14881 tagttaacca ccaggctgat acgctcaacg tccaggccac gggctgcaac gtcggtcgca
    14941 atcaggatgt ccagacgacc atctttcagg cgttccagtg tctgctcacg cagcgcctgg
    15001 ttcatgtcac cgttcagcgc ggcgctgttg tagccgttac gctcaagagc ttcagccact
    15061 tccagagtcg catttttggt acgaacgaag ataatcgccg catcaaaatc ttccgcttcc
    15121 aggaaacgta ccagtgcttc gtttttacgc ataccccaga cagtccaata gctctggctg
    15181 atgtcaggac gggtagtcac gctggactga atgcgcactt cctgcggctc tttcataaag
    15241 cggcgggtaa tgcgacgaat cgcttccggc atggttgcag agaacagagc ggtctgatga
    15301 ccttccggga tctgcgccat aatagtttca acgtcttcga tgaagcccat gcgcagcatt
    15361 tcgtcagctt catccagaac cagaccgctc agtttagaga ggtccagagt gccacgtttc
    15421 aggtggtcca gcagacggcc cggagtaccg acaacgatct gcggcccctg acgcagggcg
    15481 cgtaattgca cgtcataacg ctggccgccg tacagggcaa ccacatttac gccgcgcatg
    15541 tgtttagaga aatccgtcat tgcttcagca acctgtaccg ccagttcgcg ggtcggtgct
    15601 agcaccagaa tctgtggtgc tttcagctca ggatcaagat tttgcagcag aggtaaagag
    15661 aatgctgcag tttttccgct ccccgtctgg gccataccca gtacgtcgcg gccattcagc
    15721 agatgtggaa tacactccgc ctgaattgga gatggttttt cgtaacccag atcgttaagg
    15781 gcttcaagga taggagcctt caggcccaga tctgcaaaag tggtttcgaa ttcagccatg
    15841 tagtacgtgt gcctcaaaat taatggcggc cagtctacat aactcatcat gaaattgatc
    15901 agcaattttc attgaaaagt gtgaaccggc tcaaagtagg tgtattaacg aacaacaacg
    15961 ccctcacccg ttaaggtgat ggcaatcaaa aaagattacg ggctgatgtg tacgtcagct
    16021 attgctggtc cgattctgcc aggtcatctt ggtcctggcc caggagcgat aattccaaca
    16081 atgcgtatcg gtgctcaaca aagttatgaa cgttgttggc aaccgccagt ttgaacagtg
    16141 ccgtggcgct gtccaaatcc cccagactta ggtagtactt acctaaatag aagttggttt
    16201 cactgagatg ctcagcgagc gaggtgttat ccgttgcgtc cgccttgagc ctttccatta
    16261 acgtttgttc gctaatgttg cccaggtaga actcgacaat gttccatccc cactgttcct
    16321 tatccgattt ttcgaagtgc tgtttcaaca cttctttagc ctgcttctca tcgagcttct
    16381 gctcggcgag ataaagccac agactacgga aaggatcatt gggatcgtct tgataaaacg
    16441 ccagcagatc atcttgcgct aacttgtcac gaccgccgta atataatgcg atcccgcgat
    16501 tcaagtgcgc gtagttgtaa gttggatcaa gctcaagtac agaatcaaac gcttcatagg
    16561 cagcatcaaa attgcctgcc tgcgttaaat atatgcctaa gtaattgaat acttcaggca
    16621 tatccggtcg gattgccagc gcttgcgaaa aatcgttacg cgctaatgcc ctcagaccga
    16681 gactatcata caacactccg cgctcatata aaagctgtgc gcgttcgtca tcggttaaag
    16741 cccgactggc aaggatttgt tccatacgtg ccagaatcac ttcctgctgt aaagtcggtt
    16801 gcaatggtac cgcgaggact tcacttttac gccaggaagt attactgcat cctgcaagcg
    16861 taagtgctgt cgcaacgaaa caccagcgca aaaaaggctt catttcccac tcccgaagac
    16921 cacggttgaa tgaacgtcct gttcccggtt gctaacaagg cgtcctgccc ggttaaaagc
    16981 cccccgccgc agcggagggc aaatggcaac cttactcgcc ctgttcagca gccggagctt
    17041 ccggtgctgc agcaggttga gactgctcag tcgcttcttt aatgctcaga cggatacggc
    17101 cctggcgatc aacttccaga actttcaccg gtacttcctg acccatctgc aggtaatcgg
    17161 tcactttctc aacgcgtttg tcagcgattt gagagatgtg gaccagacct tctttaccgc
    17221 cgccgatggc aacaaatgcg ccgaagtcaa cgatacgggt cactttacca gtgtagacgc
    17281 ggcccacttc aatttctgca gtgatctctt cgatacgacg aatagcgtgt ttcgctttct
    17341 cgccatcggt cgctgcgatc ttcacagtac cgtcatcttc gatttcgatg gtggtgccag
    17401 tttcttcggt cagggcacgg atcacagagc cgcctttacc gataacgtct ttgatcttgt
    17461 ccgggttgat cttgatggta tggatacgcg gagcgaactc agagatatcg ccacgtggcg
    17521 cgttgatcgc ctgttccatt acacccagga tgtgcagacg cgcaccttta gcctggttca
    17581 gtgcaacctg catgatctct ttggtgatac cttcaatttt gatatccatc tgcagtgcag
    17641 agataccgtc gcgggaaccc gcaactttga agtccatatc gcccaggtga tcttcgtcgc
    17701 ccaaaatgtc agacagtaca acgtagttgt cgccttcttt caccagaccc attgcgatac
    17761 ccgcaacggc agctttgatt ggcacacctg cgtccatcag cgccagagac gcaccgcaca
    17821 cggaagccat agaagaagaa ccgttggatt cagtgatttc agacacaaca cgtacggtgt
    17881 acgggaattt gtccatatcc ggcattactg ccagcacgcc gcgcttcgcc agacgaccgt
    17941 gaccaatttc acgacgcttc ggagaaccga ccatgccggt ttcgcctacg gagtacggag
    18001 ggaagttgta gtggaacagg aaggtatcgg tacgttcgcc catcagttca tcaagaacct
    18061 gcgcgtcacg agcagtaccc agcgttgcgg taaccagcgc ctgcgtttca ccacgggtga
    18121 acagcgcaga accgtgagta cgcggcagca cgccagtacg cacatccaga ccacggatca
    18181 tatctttttc acgaccatcg atacgcggtt cgcctgccag tacgcggcta cgaacaacgt
    18241 ttttctcgat cgcgtgcaga atttcaccca gttcgttttc gtccagggtt tcgtcttcag
    18301 caagcagcgt cgcgatggtt tcagatttga tgacatcaac ctgcgcataa cgctcttgtt
    18361 tgtcggtgat gcggtaagca tcgctcagac gagcttcagc cagtgcagca acgcgcgcgt
    18421 tcagcgcttc gtttaccggc tccggctgcc agtcccaacg cggtttaccg gcttctttca
    18481 ccagttcatt gatgttctga ataacaacct gctgttgttc atgaccgaac actactgcgc
    18541 ccagcatctg gtcttcgctc agcagttcag cttcagattc aaccatcagt acagcggctt
    18601 cagtacccgc aacaaccaga tccagtttgc tctctttcag ctcgtcctga gtcgggttca
    18661 gtacgtactg gtcattgatg taacctacgc gggcagcacc aatcgggcca ttgaacggaa
    18721 taccagacag agacagtgct gcggaagcac caatcatcgc gacgatatcc gggttaacct
    18781 gcgggttaac agaaaccacg gtggcgataa cctgaacttc gttgacgaag ccttccggga
    18841 acagcgggcg aatcgggcgg tcaatcagac gcgcgatcag ggtttcgcct tcgcttggac
    18901 gaccttcacg acggaagaag ctacccggga tacgaccagc agcgtaggta cgctcctgat
    18961 agttaacggt cagtgggaag aagtcctgac ctggtttggc ttttttctgg ccaacaacgg
    19021 taacaaatac cgcggtgtca tccatgctaa ccataacagc ggcagtagcc tgacgagcca
    19081 tcatgccggt ttccagagtc acggtgtgtt ggccgtactg gaatttacga acgatcggat
    19141 taagcaatgt aatatccttt ctctttctta gacagtacct tacggcactg gtgttaatac
    19201 ccgatcttct gcgcatcctc gcgactaatg acaaccctaa cccagctcta tgtgggtaaa
    19261 gcctctcatt agccgcgcga acctctgcaa cggaagatca ttcatagcaa caatacatta
    19321 gtttccagtg aattgctgcc gtcagcttga aaaaaggggc cactcaggcc cccttttctg
    19381 aaactcgcaa gaattagcga cgcagaccca ggcgctcgat gagccgggtg taacgtgcta
    19441 cgtctttacg tttcaggtag tcgagcagtt tacgacgctg agaaaccatg cgcagcagac
    19501 cacgacggct gtggtgatct tttttgtgct ctgcaaagtg gccctgcagg tggttgatct
    19561 gtgcagtcag cagtgctacc tgaacttcgg tagaaccggt gtcgtttgcg tcacgaccaa
    19621 actcagaaac gattttagct gttgcttcag tacttagaga cattttaaaa ctccaaagta
    19681 tatagaatga aaggacgccg atctctaatt cagcgatccc agtgtacgtt acgcaaagtg
    19741 ttaaacaatt tacgcgacgt taagcggcag tattctactc gtagcgacct gttatcgcaa
    19801 gacggttaac attacgccgg gtattcaacc accagacgac gaggcgcaac gcggccttca
    19861 tcgtcaattt cgcccatacc gataaacttg ccgttctcac cttccgtaac gcgaaccagt
    19921 ccttccagcg gcgcgccaga tgtacgaacc gggttaccat ttttgaagta aacagaagac
    19981 gttaacggaa gattcaccac cgggtagtcc gaagctggac tgtccattgg catcagtaat
    20041 ggatcaagca actccgcagc cggaatatcc tgctgttcgg cttgttcgac cagttcacgc
    20101 aggtgctcca gggtcaccat ccgttcaacc ggatatttac ttaccgccag acgacgcagg
    20161 taaatcacat gcgcgccaca gccgagtttt tcacccaggt catcaatgat ggtgcggata
    20221 taagtgcctt ttgagcagtg aatttccagc tccagctcat tgccttcatg gcgaataaac
    20281 agcaattcat aaacggtaat tggacgcgct tcacgcggaa cttcaatgcc ctgacgcgca
    20341 tattcgtaca gttttttgcc ctggtatttc agtgctgaat acatcgatgg gatctgttcg
    20401 atatcgccac ggaaagtatc cagtgccgcc gccagttgtt ctgcactaaa ggttaccgga
    20461 cgctcttcaa cgatttgtcc gtcggcgtca gaagtatcgg tacgctgtcc aagacgcgca
    20521 atgacccgat agcgtttgtc ggagtccagc agatactggg aaaacttcgt cgcttccccg
    20581 aggcaaatcg gcaacatgcc ggtcgccagc gggtccagcg caccggtatg cccggcacga
    20641 ttggcgttat atatacgttt cactttttgc agcgcatcgt tgctggacat accctgcggt
    20701 ttatccagca acaaaacgcc gttaatgtcg cgaccgcgac gacgaggacg actcattagt
    20761 cctccttgct gtcgtccggg ttaacacgac gttcttcgtc atgtttgacc acgctggtca
    20821 ccaggtttga catacgcatc ccttcaacca gagagttgtc gtagaagaag gtcagttccg
    20881 gcacgatacg caggcgcatc gctttaccca gcaggctgcg gatgaaacca gaagcttcct
    20941 gcaacgcttt gatgcccgct ttaaccgcgt cttcatcttt gtcgttgagg aaagtcacat
    21001 acactttggc atacgccagg tcgcgagaca tttcgacacc ggaaacggtg gtcatcatgc
    21061 ccagacgagg atctttaatt tcacgctgca ggatgagagc gatctctttt tgcatttcct
    21121 gcgctacgcg ctgcgggcga ccaaattctt tcgccataat aaattctcct gacaaaaaag
    21181 gggctgttag ccccttttta aaaattaatt tcaggtggaa gggctgttca cgttggcctg
    21241 ataagacgcg ccagcgtcac atcaggcaat ccatgccgga tgcggcgtaa acgccttatc
    21301 ccgcatggaa ccctaaaaac cttaagcgat ggtacgttgg atctcgatga tttcgaatac
    21361 ttcgatcaca tcgccagtgc ggacgtcgtt gtagttctta acgccgatac cacattccat
    21421 accgttacgg acttcgttaa cgtcatcttt gaagcggcgc agggactcca gctcgccttc
    21481 atagataacc acgttgtcac gcagaacgcg gatcgggttg tgacgtttaa ccacaccttc
    21541 ggtaaccata cagcctgcga tggcaccaaa tttcggcgat ttgaacacgt cacgaacttc
    21601 cgccagaccg ataatctgct gtttcagttc cggagacagc ataccgctca tcgccgcttt
    21661 cacttcgtcg atcaggttat agatgacgga gtagtaacgc agatccaggc tttccgcttc
    21721 aatcacttta cgtgcagagg catcagcacg tacgttgaag ccaaccagga tggcgttgga
    21781 cgccgcagcc aaggtggcgt cggtttcggt gataccacct acgccagaac cgatgatctt
    21841 cactttaact tcgtcagtag acagtttcag caaggagtcg gagatcgctt cgacagaacc
    21901 ctgtacgtct gccttcagga cgatattcac ttcgtgaact tcgccttcgg tcatgttggc
    21961 gaacatgttc tcgagtttag atttctgctg acgcgccagt ttaacttcgc ggaatttacc
    22021 ctgacgatag agtgcaactt cacgcgcttt cttctcgtca cgtacaacgg taacttcatc
    22081 acccgcagcc ggaacaccgg acaagccgag gatttccacc ggaatggacg gacccgcttc
    22141 cagcacttcc tgacccagtt cgttacgcat cgcacgaaca cgaccgtatt cgaagccaca
    22201 cagaacgata tcgcccttgt gcagagtacc ttcacgtacc agaacggtag caaccggacc
    22261 acgaccttta tcgaggaagg attcgataac cgcaccgctc gccatacctt tacgtaccgc
    22321 tttcagctcc agaacttccg cctgcagcag gatagcgtcc agcagttcat cgataccggt
    22381 acccgctttc gcagatacgt gtacgaactg gctttcaccg ccccactctt ccggcaggat
    22441 gccgtactgg gagagttcgt tcttaacgcg atccggatcg gcttctggtt tatcgatctt
    22501 gttcactgca accaccaccg gtacctgcgc cgctttcgcg tgctggattg cttcgatggt
    22561 ctgcggcatt acgccgtcgt cggcagcaac aaccaggact acgatgtcag ttgcctgcgc
    22621 accacgtgca cgcattgaag taaacgcggc gtgccccggg gtgtccagga aggtgatcat
    22681 gccgttttca gtttcaacgt ggtatgcacc aatgtgctgg gtaatgccgc ccgcttcgcc
    22741 agaggccact ttcgttgaac gaatgtagtc cagcagagag gttttaccgt ggtcaacgtg
    22801 acccatgatg gtcacaaccg gagcgcgcgg ttcagccgca gctcccgtgt cacggtcgct
    22861 cattaccgcc tcttccagct cgttttcacg acgcaggata actttgtggc ccatctcttc
    22921 agcaaccagc tgtgcggttt cctgatcgat aacctggttg atggttgcca ttgcgcccag
    22981 tttcatcatc gctttgatga cctgagagcc tttaaccgcc atcttgttcg ccagttcgcc
    23041 aacggtgata gtttcgccga tcacaacgtc acggttaacg gcctgagcag gcttctggaa
    23101 gccttgctgc agcgaagaac ctttacgttt tccgccctta ccgccacgta ctgctgcgcg
    23161 tgcttcttca cgatcagctt ttgattcagc gtgtttgttg cctttcttcg gacgcgctgc
    23221 tttcgcgtta cgaccacggc cacggccgcc ttcgacttca cgatcgcttt cgtcttctgc
    23281 ctggcgagca tgttgagaag tagtgacgtg ataatcgctg gaatcctcag tcggttccgc
    23341 gttatcagtc catttgtttt cttccgccat acgacgtgct tcttcagcaa cgcgacgtgc
    23401 ttcttcttcg agtttacgac gcgcttcttc ttcggcttta cgcttgagct ctgcagcttc
    23461 ctgctcacgg cgggcttttt cagcctgggc gtttttagtc atatcgtctt gttgattgct
    23521 cactttgtct ttttccgcag cttcacgttt cgcttgttca gcagcttcac gcttagcttg
    23581 ttctgcggcc tcacgttcag ctttttgttg cgcctcgcgt ttagccgatt cttctgcctc
    23641 acgacgggct tgctcttccg cttcacgctg cgcttgctct tccgctgcaa ggcgttcagc
    23701 ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg acttcgattt gtaccgattt
    23761 gctttttcca ccggtaccag gaatgttaag ggtgctgcgt gttttacgtt gcagcgtcaa
    23821 tttgtccggg cctgaatttt tctgattcag gtggtcaatc aaagtctgtt tctcttgtgc
    23881 agacacagag tcgtcagcag acttccggat acctgcatca gcaaattgct gtaccaggcg
    23941 ttccacggag gtctgtcgct ctgcggccag cgttttaatc gttacatctg tcatgctgtt
    24001 ccttcctgct acagtttatt acgcttcgtc accgaaccag caaatattac gggcagccat
    24061 aatcagtgct ccggcttttt cgtcggtcaa cccttcgata tcagccagat catcaatgcc
    24121 ctgttcggcg agatcttcca gcgtacaaac gccacgggcg gccagtttga atgccaaatc
    24181 acgatctacc ccttcaaggt tcagcagatc gtcagccggt ttgttatcac cgaggctttc
    24241 ttcctgggcc tgtgcaatgg tggccagtgc atttttagca cgctcgcgca gtgcttcaac
    24301 ggtcggctca tcaaggcctt cgatttccaa cagctctttc atcggcacat aggccagttc
    24361 ttccagcgtc gagaagcctt cctctaccag aacagtcgcg aagtcttcgt cgatgtcgag
    24421 atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc tgatgcttag cctgcaggtc
    24481 gtcaacggtc atcacgttga gttcccaacc gctcagctgc gaagccagac gcacgttctg
    24541 accgttacgg ccaatcgcct gcgccaggtt accggcttca acggcgatat ccatggtgtg
    24601 tttatcttca tccaccacga tagaagcaac gtctgccggt gccattgcgt taatcacaaa
    24661 ctgcgccggg ttatcatccc acaggacgat atcgatacgc tcgccgccca gttcagtaga
    24721 aaccgcctga acacgcgcgc cacgcatacc tacgcaagca cctaccggat cgatacgttt
    24781 atcgttggtt ttcaccgcga ttttcgcacg agaacccggg tcgcgagccg ctgctttaat
    24841 ttcaatcact tcttcgccga tttctggcac ttcaatacgg aacagttcga tcagcatttc
    24901 cggcttggaa cgagtgacga acagttgcgc gccacgcgct tccgggcgaa cggaataaag
    24961 cacgccacga acgcggtcgc cagggcggaa gttttcacgc ggcagcatat cttcgcgcag
    25021 gatcacggct tcagcgttgt tgcccagatc cagagagatg ttgtcgcggt ttactttttt
    25081 caccacgccg gtgatgattt caccttcgtg ttcacggaac tgatcaacca ccatcgcacg
    25141 ttcggcttca cgcactttct gcacgataac ctgttttgcc gtctgggtgg tgatacggtc
    25201 aaaggtaaca gactcaatct gatcttcaac gtaatcgccc aggttcaggc tttcatcttc
    25261 ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg gtgacttcat caacaactaa
    25321 ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg atctgtacgc ggacgtcgat
    25381 ctcttgttca tattttttct ttgttgctgt cgccagcgcg ctttccaatg cttcgaaaat
    25441 cttctcgcga ggtagcgcct tttcattgga tacggcttca actacagcca aaatttcttt
    25501 gttcatcgcg ggcttttcac ctcatccaga ctattaaaag tggggaacca agttcgcctt
    25561 ctggatatta ctcagcgcga acacttcatc tttaccttcg acggtaactg tgatcatttc
    25621 accgtctacc gctttgataa cgccctgcca tttacgacgg ttttgtaccg ccatacggag
    25681 aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt tcagccgtga acagtgggcg
    25741 atcgagaccc ggtgaggaga cttccaggtt ataagcaacg gtgatgggat cttcaacatc
    25801 cagcacagca cttacctggt ggctcacatc agcacaatca tcaacattga tgccatcttc
    25861 actatcaata tagatgcgca gtgtggatgt gcgaccgcga ataaattcga tgccaaccag
    25921 ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct gttaattttt gctctaatgt
    25981 ggacaagccc acccccaaga cataaaaaaa gggcctaaag cccagttatt ctgtagtcag
    26041 ataacaaaaa accccgataa atcggggctt tatataactg aaccctataa ccgcaactgc
    26101 ggtctggagc actttccaga aggatttttt caaatcccac tacgaaggcc gaagtcttca
    26161 cagtatattt gaaaaaggac tctaagggaa agtggttgcg ggggccggat ttgaaccgac
    26221 gaccttcggg ttatgagccc gacgagctac caggctgctc caccccgcgc ctgaaacgtg
    26281 gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc tgggatttgg tgtaccgaga
    26341 cgggacgtaa aatctgcagg cattatagtg atccacgcca cattttgtca acgtttattg
    26401 ctaatcacgt gaatgaatat ccagttcact ttcatttgtt gaatactttt gccttctcct
    26461 gctctccctt aagcgcatta ttttacaaaa aacacactaa actcttcctg tctccgataa
    26521 aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag cggaaatcca
    26581 ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct caagcatctc
    26641 ccggtaggtc aacgtattgg tatcgctttt tccggcggtc tggacaccag tgccgcactg
    26701 ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct gggccagcca
    26761 gacgaagagg attatgatgc gatccctcgt cgtgccatgg aatacggcgc ggagaacgca
    26821 cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat tcagtgtggc
    26881 gcatttcata acaccaccgg cggcctgacc tatttcaaca cgacgccgct gggccgcgcc
    26941 gtgactggta ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac
    27001 ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc gttatggtct gctgaccaat
    27061 gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga actgggcggt
    27121 cgtcatgaaa tgtctgaatt tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa
    27181 aaagcttact ccacggactc caacatgctt ggtgcaacgc atgaagcgaa ggatctggaa
    27241 tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg gcgtgaaatt ctgggatgag
    27301 agcgtgaaga tcccggcaga agaagtcaca gtacgctttg agcaaggtca tccggtggcg
    27361 ttgaacggta aaacctttag cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc
    27421 ggtcgtcacg gcctgggcat gagcgaccag attgaaaacc gtatcatcga agcgaaaagc
    27481 cgtggtattt acgaagctcc ggggatggca ctgctgcaca ttgcgtatga acgcctgttg
    27541 accggtattc acaacgaaga caccattgag cagtatcacg cgcatggccg tcagttgggc
    27601 cgtctgctgt accaggggcg ttggtttgat tcccaggcgc tgatgctgcg tgactctctg
    27661 caacgctggg ttgccagcca gatcactggt gaagttaccc tggagctgcg ccgtgggaac
    27721 gattattcaa tcctgaatac cgtctcagag aacctgacct acaagccaga gcgtctgacg
    27781 atggaaaaag gcgactcggt gttctcgcca gatgatcgta tcggtcaact gaccatgcgc
    27841 aacctggata tcaccgatac ccgcgagaaa cttttcggtt atgccaaaac tggcctgctt
    27901 tcctcctctg ccgcttcagg cgtgccgcag atggagaatc tggaaaacaa aggccagtaa
    27961 ttcgacatca accctgccct tcaggggtgg ggttaccttg cttatcctct gtaaactcct
    28021 ttctgagcat cacataatcg aaaacatcat ggtttgcatc cacaaccagt gagtctcctt
    28081 tatagtgtac aatcacttcc tcaagcgttt gcggctgttc cggctttgtt acccccatcc
    28141 acgcattaat aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc gttgtgcgat
    28201 ccacaccatc cccaagaacg gggctatacc agataaacat cggaacatga tatgcctgct
    28261 ggctggcctc cctgcctcca tgaaaataga cgttcttctt cgtagggtca cgttccaggc
    28321 cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt taataattca aaaacctgac
    28381 ctagcaaact atctgtgtaa tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg
    28441 gttgaaacac ggcgctggat tgcggatagg cgctacaagc cggttcatgg cttccattta
    28501 aatgaagaac aatcagtttc ttttgctgcg tattttgctg taatgcttgc gataaatgcg
    28561 gcaacaacaa ttcatcaaat cctctgacat aaactgtttc catggcacgc atggcgatac
    28621 tggtaactgc tgtaccattc tgccgaaaag cggattgcga gcttagccag aaagtctgaa
    28681 atcctgcttg attagccata ttaataatgt tgtcggggta attatgaatg tcatggctca
    28741 aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt gcaccgctta
    28801 ttgcttgatt aaacagtttg atctgttttc tttgtgcttc gacttgcggt gtcgtagagc
    28861 gtgtatatcc atacaaagac atattgtcga cacgtacaga ctcccccaca atcaacacgt
    28921 aggtatcaat acctgtatcc ctgactgata attgaaaata cggcactgtg tttgcaattg
    28981 agagtaatct ttgatgctct ttcgctgcta aagcaaaata attgagattg aaaaacggcg
    29041 tataggtagc aaatcgcgac gctagtatat atggactgaa cgcattttta tttttggcat
    29101 ctttataagc aaattgacaa gcggaaaata aacttcccga aatgacaatc agcaataata
    29161 ttcctgtcac tttttttgtc ggcaaggaaa catcatattt tattattaca gccaaaaaaa
    29221 gaagggataa aaaggcaaaa gcacatagat aagggctata cattcccagc attttggcaa
    29281 cttcatccgg atcactctgc agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa
    29341 aggtacacca tgaatagagg ctaatactca tgtctgccgc agtcagtaac acaaatggtg
    29401 cggcaacaag caggcgaagc ggaagggatg atatacgttt aacaaccagg atggtcagaa
    29461 cgaagaacag caatcgctga accatatgcg ctccggaaga ggcgactaag ttcgtaatac
    29521 caaaaacaat aacactaaga tacaacaacc aatgagattt aaaacttcta gcaaatttgt
    29581 tgaatactgt catccagaaa tccaagttca caaccaatat aaacaattat caacaatgtg
    29641 caattttata tcagaca
//
LOCUS       DAZFBR010000057        28801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFBR010000057 DAZFBR010000000
VERSION     DAZFBR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.057"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..283)
                     /locus_tag="ACHJZ2_002692"
     CDS             complement(<1..283)
                     /locus_tag="ACHJZ2_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568775.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSG"
     gene            complement(287..2407)
                     /gene="ehxB"
                     /locus_tag="ACHJZ2_002693"
                     /gene_synonym="hlyB"
     CDS             complement(287..2407)
                     /gene="ehxB"
                     /locus_tag="ACHJZ2_002693"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO4568776.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRITKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFIRELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            complement(2457..5453)
                     /gene="ehxA"
                     /locus_tag="ACHJZ2_002694"
     CDS             complement(2457..5453)
                     /gene="ehxA"
                     /locus_tag="ACHJZ2_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO4568777.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARIISELGDKDDVVYSGAGSSEVFAGEGYD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYGQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            complement(5455..5970)
                     /gene="ehxC"
                     /locus_tag="ACHJZ2_002695"
                     /gene_synonym="hlyC"
     CDS             complement(5455..5970)
                     /gene="ehxC"
                     /locus_tag="ACHJZ2_002695"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO4568778.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            complement(6511..7682)
                     /locus_tag="ACHJZ2_002696"
     CDS             complement(join(6511..7433,7433..7682))
                     /locus_tag="ACHJZ2_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4568779.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
     gene            complement(7861..8262)
                     /gene="eptO"
                     /locus_tag="ACHJZ2_002697"
                     /gene_synonym="gspS"
     CDS             complement(7861..8262)
                     /gene="eptO"
                     /locus_tag="ACHJZ2_002697"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFO4568780.1"
                     /translation="MMGNILKKLNCIASLLVLVTISGCHQSPSIHKQATVPPSEQLEQ
                     MASIVSATRYLKMRCNRSDLPDEQSILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRL
                     TRDSTPEYIKCREFNRRLVPFIGELLARGRG"
     gene            complement(8354..9124)
                     /locus_tag="ACHJZ2_002698"
     CDS             complement(8354..9124)
                     /locus_tag="ACHJZ2_002698"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO4568781.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            complement(9245..9757)
                     /gene="gspM"
                     /locus_tag="ACHJZ2_002699"
     CDS             complement(9245..9757)
                     /gene="gspM"
                     /locus_tag="ACHJZ2_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO4568782.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            complement(9744..11021)
                     /gene="gspL"
                     /locus_tag="ACHJZ2_002700"
     CDS             complement(9744..11021)
                     /gene="gspL"
                     /locus_tag="ACHJZ2_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO4568783.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            complement(10955..11932)
                     /gene="gspK"
                     /locus_tag="ACHJZ2_002701"
     CDS             complement(10955..11932)
                     /gene="gspK"
                     /locus_tag="ACHJZ2_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO4568784.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            complement(11929..12528)
                     /gene="gspJ"
                     /locus_tag="ACHJZ2_002702"
     CDS             complement(11929..12528)
                     /gene="gspJ"
                     /locus_tag="ACHJZ2_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO4568785.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            complement(12525..12890)
                     /gene="gspI"
                     /locus_tag="ACHJZ2_002703"
     CDS             complement(12525..12890)
                     /gene="gspI"
                     /locus_tag="ACHJZ2_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO4568786.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            complement(12887..13441)
                     /gene="gspH"
                     /locus_tag="ACHJZ2_002704"
     CDS             complement(12887..13441)
                     /gene="gspH"
                     /locus_tag="ACHJZ2_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO4568787.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            complement(13438..13872)
                     /gene="gspG"
                     /locus_tag="ACHJZ2_002705"
     CDS             complement(13438..13872)
                     /gene="gspG"
                     /locus_tag="ACHJZ2_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO4568788.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            complement(13903..15126)
                     /gene="gspF"
                     /locus_tag="ACHJZ2_002706"
     CDS             complement(13903..15126)
                     /gene="gspF"
                     /locus_tag="ACHJZ2_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO4568789.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            complement(15128..16633)
                     /gene="gspE"
                     /locus_tag="ACHJZ2_002707"
     CDS             complement(15128..16633)
                     /gene="gspE"
                     /locus_tag="ACHJZ2_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO4568790.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            complement(16633..18600)
                     /gene="etpD"
                     /locus_tag="ACHJZ2_002708"
     CDS             complement(16633..18600)
                     /gene="etpD"
                     /locus_tag="ACHJZ2_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFO4568791.1"
                     /translation="MFRKWLNGRLPVLVFTTVILGAIPGWGAEFSANFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDVSPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVMKRLMEIVERVDKVGNRSVATVPLTYASATDVARLVTELTKET
                     DKTAIPAWMTAKLVADERTNSVLVSGEPISQQRIISIIKQLDRQEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSSIENDSKKSPSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEIQDADGLNLGIQWVNKHAGVAQFTSTGLPITTMVQTR
                     QNEILDSDQSNALSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFRAKSEQTAKRNLMLFIRPTIIRERDGFRHASAEKYQSFNQEQVQSRGKETTALTL
                     NEEQLRLSPDQDDTAFRKVKAAIAAFYAQEM"
     gene            complement(18601..19476)
                     /gene="gspC"
                     /locus_tag="ACHJZ2_002709"
     CDS             complement(18601..19476)
                     /gene="gspC"
                     /locus_tag="ACHJZ2_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO4568792.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDATISAIFSDHIV
                     INYQGKTVSLPLRYDNTEKKNAYDNNNLTVGDVITQDNFRVESVFDIMSFSAVTVNNT
                     LSGYRLIPGKHSSLFYNAGLHDNDLAVSVNGSELRDTRQAQQIMKQLPELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            complement(19563..22259)
                     /gene="stcE"
                     /locus_tag="ACHJZ2_002710"
     CDS             complement(19563..22259)
                     /gene="stcE"
                     /locus_tag="ACHJZ2_002710"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFO4568793.1"
                     /translation="MNTKMNERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYF
                     NTSQPINDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTP
                     VQVEARDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVN
                     KLSDASGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTV
                     FYGDRKVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLV
                     IKQGNLSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSR
                     MIVNNYAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLN
                     STAGLGENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGH
                     NYGLGHYVDGFKGSVHRSAENNNSTWGWDSDKKRFIPNFYPSQTNEKSCLNNQCQEPF
                     DGHKFGFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADT
                     QEMEPYEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASA
                     DNRGSILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPE
                     QFGVPVTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQL
                     RFRLANHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQ
                     ALPAKENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDN
                     LSYNRIGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
     gene            22699..22785
                     /locus_tag="ACHJZ2_002711"
                     /pseudo
     CDS             22699..22785
                     /locus_tag="ACHJZ2_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(22889..23077)
                     /locus_tag="ACHJZ2_002712"
                     /pseudo
     CDS             complement(22889..23077)
                     /locus_tag="ACHJZ2_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23130..24128)
                     /gene="lpxM"
                     /locus_tag="ACHJZ2_002713"
                     /gene_synonym="msbB"
     CDS             complement(23130..24128)
                     /gene="lpxM"
                     /locus_tag="ACHJZ2_002713"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO4568794.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            complement(24202..25923)
                     /gene="cptA"
                     /locus_tag="ACHJZ2_002714"
     CDS             complement(24202..25923)
                     /gene="cptA"
                     /locus_tag="ACHJZ2_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO4568795.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            complement(26013..27119)
                     /locus_tag="ACHJZ2_002715"
     CDS             complement(26013..27119)
                     /locus_tag="ACHJZ2_002715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4568796.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            complement(27119..27940)
                     /locus_tag="ACHJZ2_002716"
     CDS             complement(27119..27940)
                     /locus_tag="ACHJZ2_002716"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO4568797.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            complement(28542..28676)
                     /locus_tag="ACHJZ2_002717"
     CDS             complement(28542..28676)
                     /locus_tag="ACHJZ2_002717"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568798.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
BASE COUNT         7718 a         7578 c         5902 g         7603 t
ORIGIN      
        1 ttccactatg agtaaactta ccatttgcta ctgaaaccac ttcaactttc ccaagaacag
       61 ataagagaag agatataatt acaaataata aaatactcca tacaacaaaa tgagaacgtc
      121 tggatactgg tgtttcaatg agttcaaggt gagcaggaag aaatgcatac tcatcttttt
      181 ctctcacagg agcatcaagt gtgtgacgaa ttttccagac atcagaaaaa accgtcttat
      241 aacggcaaac caaatcccat aaacctttca tgtaaaagcg catacgttat gcctgcaact
      301 gatataaata tgcatataaa ctgtcttttt tggagataag ctctttatgt gtgccatctt
      361 cagaaataaa gccattatcc ataacaatta ttctgtttgc attttttaca gttgacaacc
      421 tgtgtgcaat aataattacg gttctgttct tacatattct tgacatattt ttcattatta
      481 tattttccga ctcataatca agagcactgg ttgcttcatc aaaaataaga attcgaggat
      541 ttgtaacaag agcccgggct attgcgatcc gttggcgctg ccctcctgaa agaccaacgc
      601 cctgttctcc aacaatagta ttgtaccctt cttttagttc tctaataaaa tcatgggcac
      661 ctgcaagtct ggctgcctca atagcctgtt ccatagatac agccggagaa gctaatgtaa
      721 tattatcgat aatactacga tttaatagta tattttcctg caatacaaca ccaatctggc
      781 gtcgtaacca ttctggatct gcaagtgata aatcatgccc atcaattaaa atctgtccgg
      841 tctctggtat ataaaagcgc tgaagcagtt ttgttaatgt actttttcct gaaccagaac
      901 ggccaactat accgataaca tccccctttg atatgtataa attaatatta ttcaaaataa
      961 cattaccgtc agaagaatac cgaaatctga catttttaaa ttcgatatcc ccctgaattt
     1021 ccggcagtat atttctgcct gactttttct ctaccggtgt atttaataca tcaccaaggc
     1081 gctcaacaga aataccaacc tgctgaaaat cctgccaaag atgagccagt ctgataacag
     1141 gagcaatgac ctgacctgca agcatattaa acgcgattaa ctgcccgatg cttatctctc
     1201 ccgatatgac aagatgtgct cccatccata gactaattac cattacactt ttctgtatca
     1261 gctgtatccc ctgctgccct gtcattgcaa ccagatttac cctgaaactg gaagcaacat
     1321 acccggccag ttgtttatcc catgtagcaa tcatctgtgg tgatactgcc atggatttga
     1381 ttgtatttat tgctgttacc gtttcgacaa gaaaagcttg attttctgca ttcctgagaa
     1441 acttatcatc aagacgtcga cgtaataagg gtgatataaa caatgaccat ataacataac
     1501 aaggtagtga caataatata accagtgtta attgagggct gtaataccac atgacacaaa
     1561 aaaatattac agaaaaaaat agatcaagaa ctgatgtcaa cgcttgtccg gttaaaaaat
     1621 tacgaatttg ctccagttcc cttactctgg caacggtctc tccgaccctg cgattttcaa
     1681 aatatgaaac aggcaatgca agcaaatgtc tgaataactt agcacccagt tcgacgtcaa
     1741 tacgacttgt agtatgagag aaaatataag ttctggctcc ggttaatatc acttcaaaaa
     1801 gtatcactat aataaatgct attgtgataa tatttaacgt tgaaaacccc cggtgaacta
     1861 aaaccttatc cattacaacc tgaaaaaaaa gaggtgttat taacgcaaga aactgaataa
     1921 aagcagaaac agttaacacc tcaagtaaaa tcctcctgta tttcacaaca gaggggataa
     1981 accaagaaaa atcaaattta gctaactctc cgactactgt tgctcttgac gtaaccagaa
     2041 tgacttttcc ctgatacagt ttttcaaact catcccgact aaaagttagt gactgatgtt
     2101 gttctggatc ataaacaaga tagcgtgatg aatctttagt aatacgagac aatatataat
     2161 gcttaccgtc atcccgccat atcaacgcag gtaaagaaat tattgacaat ctggaaaaat
     2221 gtttttctac atattttgct tttaagccaa tagatttcgc tgccagtaac cattcagtca
     2281 ccccaaaatc ttgtgtgtgg gtattatact gatgccttat agtttcagca ttgacagtta
     2341 tattatgata ttgtgcaagc aatatcagtg catacagact attatgagaa ctacatttac
     2401 tcatcatata acgatgacca ttcctcctgg aatggccatc acctcctctt ttagtctcag
     2461 acagttgtcg ttaaagttgt tgagtgtgtg ttgttttgat gtaaggataa attatataaa
     2521 gatgctaatc gttcatcacc tgaatcgaac cctgaaactg aagaaataat cttattaata
     2581 tcattggcaa cactggtgat attaaccatg tttttttcag atgctatatt actggctttt
     2641 atataacctg accgttcatt attattatga ggtatcttat caacttttat ctctcgacca
     2701 tctttatctg ttataacctc aacaagatga ttatctgctc ctgaggcatc tttcgcaaac
     2761 cagtttttaa atgttatccc attgacatca tttgactcat taaatgaaag tacaccatta
     2821 atggctttat tcatgataag atcatttcca actctcttaa atgcgatatc atcaaagcta
     2881 atatcagata agtgcaaacg atctccttta cctccttcat ctgcaattgt atgatgacca
     2941 tagttttgcc cataaacata aatatcatta ccaaaaccac cattcagata gtcattacct
     3001 tcccctccat ccagaagatc aataccacca ccaccataaa gtttatcatc accagtacca
     3061 cctgacagaa tattataagc tccgtgtgcc tgaagctcat cattaccgct tcctcctgtc
     3121 agataattat taccatcccc tccactcaat ttatcgtttc cactaccacc aaataactgg
     3181 tcgtctccct gtcctccgga tatataatca tccccatcat cgccgtatag tcgatcatta
     3241 ccataattac cttcgatata atcgttccca tctgcgccat ggaatatatc attaaactta
     3301 ccgcctttga attcatcatc atgttttccg ccaatgagct cttcaacaga atgaagatta
     3361 tctattacat cataaggaat tccaccggtt ctgaattcaa aatcacgata ttgtattttc
     3421 tctgttcgct tccctactga cacctcctgt tccttaacga cttcctgcaa taccttcacg
     3481 tcaccataca tattttttga aactatatac tcaccaggtt ttgatgctcc tgttgcatca
     3541 attgttagtt taccaacatc cgtcttatta taagatacgg tatcataacc ttcaccagca
     3601 aatacttctg atgagcctgc tccagaataa accacatcgt ctttatctcc gagctcagaa
     3661 attattctcg cctgatagtg tttgttattc ttttcaacaa actgaatcaa ttttgaataa
     3721 tcatatacac ctttatgatt ttttatgcct tttacagacc atgtatcctt accatttaca
     3781 ataagagatg tcatatattc atatttacca ctctgttttc tttctctaac ttcttctcct
     3841 ggggtaaatg ttggtgttat aaatgtcaaa acacttgata cattacctgt tgaaaggtct
     3901 atggtccctt tttgaggatc aaacacttgt tgtgtaaact ccttcggttg agcctctaaa
     3961 agccctccat tttccagata attaatatat gccttaccac tctgactgcg atcagcatta
     4021 cgggttatgc ctgcaagttc accgatcttc tcatcccaat gttgctgggt tattgcgact
     4081 gctctttcta ctgcatgctg acgagaaaaa tcagcaagca aagacagaga gtcttctaaa
     4141 aacgcagcat gtcttgcgtc atatccattc tcaaaataat ttttgccatg ctccttttcc
     4201 cattcattga tccgagcagc gaatttctct gcaacgtgct caaacatagc ctgttttgat
     4261 gcttccagaa tgccagatat cgtaccggtt aatgcactca ccagcatgct tatcggggcc
     4321 cctatgaggg atgcactgga ggctgcacta actcccgcag atacagaact caggacagta
     4381 tttattgttg tcagggctgc atctatagct ccggtttctt tatgaaaggc tgcgagtaaa
     4441 gcatcccctt cataattcaa ttttttaaat cgttcagaat atgattcaag ctgcttagct
     4501 cgctcaaatt tatctgcagc agccaggaaa gaaagaggac tgatagccag cataacagcc
     4561 gatgtgatca gacccgcact tgcagctgtt gtcgataacc cctgtgccat tctctgagcc
     4621 agaatatatt gcgaaacagc tttaccaaca tttccaagaa cctgagttgt cagttcgata
     4681 cccgctgcag cttttgttcc tgtatgtgcg tcactgtttc ccagaataaa gcttgctgat
     4741 acagcagaaa gaattccgga gacaacatcc agcccatccc ccagggggcc caggtctggt
     4801 aaattttgta atttcccacc aacagaactt aatcgaggtt tactggataa aaatgagcca
     4861 agctggttaa gctgctcaga aaatgaatca acggtactgt ttatacttga tactgtatct
     4921 acaagctgat taataagttc aatactactt ttggcaatat cattctgact tatatcctct
     4981 ccttcccgtt gttttctcag caattcatca agagccatgc ctgataaagc aatccccgta
     5041 aaattctgta gtgctgagag aactgttccg gcttttccca gattattacc tacattttca
     5101 gcggttcctc ctattttact cccaactttc tgatacttct gcagaagttt gtcaagttgt
     5161 ggtgcgaata ttgcaacacc acgttcagtt aatcctacaa ctttttctgc tgcaccaaaa
     5221 aactggtttg caatcgctgt atcatctcgt tcagtacggt gtatttctat tccaagctca
     5281 tcagcagctt tgaccaactc attaataccc acgccctgag cttcataatt atcaggtatt
     5341 aataatatga gtttttttcc agcggaacgt acgcttgatg atgctgtatt aaatgctgat
     5401 tttgtagtca atgtcgcatt attgaaaatg ttctttattt tatttactgt catattagtt
     5461 ttctttctca atattaaaat taaaatcttg tctgtttttt acttcagaca taagttcatg
     5521 atggtattgc ctgaatattt ttgatgccag ttttttatct atcccccctc catgaaattc
     5581 agctattttt cctgttttac tgctctcatc taaccttatt gctctgaata actcataagg
     5641 atactttttc ctcatgtatt tatataactc catgttgtgt ccaaatggag ctatccagtc
     5701 aataaaccac tttctttctc ccgacatcca gtcttttgca tacaaagatg ttacatcatt
     5761 tatatatttt acctcacatt ccagatctaa actggcccag ctacaaaaag ctacaggaag
     5821 ctcatccttt attaagagag caaattgatt tgtctgaata gcaggtatga cattaatagc
     5881 aaaaacagat aatggccatt ttttatgcag aggagaacag gcccatagcc atgcaacctt
     5941 ccccatgacg tcaaaagcat tagatttcat aatgtttaaa taaataagaa aactcagtaa
     6001 atgtaaagaa ccctatataa aagaacagaa attatatcct tttaagcaag aagcatctaa
     6061 aaacagaaag ctaccgcaag gagaatctct cccaaccaaa acaacattag cgataataat
     6121 atattgccta aacattctta cacatatcac agctaagtta tattataaca ctcagatgat
     6181 tgatttgtaa gataacgtat gattttcata tgaatagcca cgtgacaaat gccttattaa
     6241 agagattgta aacagaatac agcaaactat ttcagttacc gaaatgacga gttaatcagt
     6301 tttgtttacc ctgcaaacac aaaatctaaa cctacacaga aaagcaaacc tctcctgtta
     6361 ataataaatc tcttccggta tagctggagt tttttcaaat gcgcctataa ggctctctta
     6421 ccagccgagc cataacaggc gcacacgatc tgacatttgc atccaacttc gttacttacg
     6481 gcccgtaaat cggctgacgg gatgtagtgg tcaacaaaaa ctggccaccg ctttagagtt
     6541 tttccagtat cggttttccg attcatttgg tggcaaccca ccgttatatt cgtgcggcct
     6601 gagcgcgttg taatacccaa cgatatagtc cgttatttca tgggcagcat cgctgaagtt
     6661 catgtaaccc gtcaccggta tccactcgtt tttcagactc ctgaagaagc gctccatcgg
     6721 gctgttatcc cagcaatttc ctcgtcgact cagactctgt ttgatctggt aacgccacag
     6781 taactgccgg aactgcctgc ttgtatagtg gctgccctga tcgctgtgga acattacccc
     6841 ggctggttta ctgcggattt cccaggccat tttcagcgct ttgatggtca gtctgctgtc
     6901 cggagagaac gacattgccc aacctaccgg tttccttgca aacaggtcga gaacaacggc
     6961 aaggtatgcc caacgtttcc ccgtccagat gtacgtcacg tcgccgcacc atacctgatt
     7021 tggctctgtc actgcgaact gccgcccaag gtgattcggg atagtgacat gttcacgacc
     7081 acctcgttta taacggtgcg caggctgctg gcaactgacc agtcccagtt ctttcatgag
     7141 cctgccggca agccagcgcc ccattctgaa gcctctcagg gttgccattg tggcgatgct
     7201 tcttgccccg gcagaaccat ggctgatgtt atgcaactca agtacctgac tgcgtaatac
     7261 agcccgtctg ccgtctggtt tttcaggacg gtttttccag tatctgtagc tgctgcgatg
     7321 aaccccgaac acatggcaga gtgtgaccac aggataatgc gctctgagtt tcccgattat
     7381 cgagaactgt tcagggagtc tgacatcaag agcgcggttg tagccttttt taatatttca
     7441 ttctccattt caatgcgttg tagctttttc ctcagctcac gtatttcgat ttgttctggt
     7501 gttatcggag aggcttttgg tgttttgccc tgacgctcat cacgcagttg tttgacccat
     7561 cttgtcattg tggaaaggcc gatatccata gctttggcgg catctgccac cgtgtagttc
     7621 tggtcaacaa ccagttgagc ggattcgcgt ttaaactctg cgctgaaatt tctttttttc
     7681 attggagcac ctgtgttgtt ctgaggtgag catatcacct ctgttcaggt ggccaaattc
     7741 agtgtgccac tacaatcatc atgccttcag ccaccactac aagggaagac ccatttttat
     7801 tcatgctgtt tacctctttc tcagggagtt tagtctccag gatttccggg gcggttcata
     7861 ttatccacga ccccttgcca ataattcacc gataaatggg accagccgcc gattgaattc
     7921 tctgcatttt atatactcag gcgtactgtc cctagtcaga cgcacataga tatcatcgct
     7981 gtgtttacgt atatcctcct gagttaatga ctgccaccct ttcccgatag cgatccggtt
     8041 agcgacattt aggatcgact gttcatctgg taaatcacta cggttacatc tcattttcag
     8101 ataccgtgtc gcagagacaa tcgacgccat ttgttccagt tgttcgctcg gaggaacagt
     8161 ggcctgctta tggatggagg gtgactgatg acaaccagag atagtcacta agactagtaa
     8221 tgacgcgata caattcagtt ttttcaaaat attacccatc attttttact aggtatcgtt
     8281 taacctgata ccggtttaca acagttcttt tatccgctga gccgttttta ttactagccc
     8341 cggtaaccga acgctacatc aacaaaaaat ggcacactcc gctcaaagcg agccacggcc
     8401 caaaaggcag cgggcgggta aagtccgtag ctgttacaat acgcaacagt actgttacga
     8461 ttaacccact gactgaggca aacaaaagta ttgagggtaa tgcctgccaa cccatccatg
     8521 ctcccaatgc cgcagctagc ttaaaatcac cgtaacctaa cccttccctc ccggtagcga
     8581 aacgaaaaag ccagtacagt ccccacagac agagataccc agataccgcc cccactaccg
     8641 cctcctctaa ggtgacaacg cctctctgta aactgaataa caggccaagc cagagcaatg
     8701 gcaacgtcag cacatccggc agcagcgccg tatggcagtc aataaccgcc agaaccaaca
     8761 gcatggaaat aaaaagccag gcagccgcta atgtcagccc tggtggaaac ctgctcgcgg
     8821 ccagaatgaa taacactgag ttcgttattt cgactagcgg gtagtaaacg ggaatacgct
     8881 cgccacaaca gcgtgatttc cccttcagaa ataggtagct taacagggga atgttatccc
     8941 tgatgccgag cggatgaaaa cagcagggac aatgagactg cggtatcatc aggttaaagg
     9001 ccggcccgga ctcaggcaca gaatgtgcca atagcgtcag cgcttcacgc cgccaccgcc
     9061 tctccaacat tatcggcaga cgatgaatca cgacgttaag aaaactgccg gtaacgcccc
     9121 ccagccctcc ggtgcagatg agccacaaca agggataatc aaccgcaaat tccggcaaac
     9181 tactcacaca tcctccttta taagtccaat ttttacatca agcattaatc tgtaaattac
     9241 gacatcagcg actaagcgaa gcgtccttgt ttacgtcatc ttgggacaaa accagccgat
     9301 taaccgttac ctgtccgggt gggttgccct gcgcggccac atcaaatacc atggtacgaa
     9361 tacggtgccg acgctccagt tgcgtaagcc actgcatcag cgcattaaat tcaaccgggg
     9421 ctaaggtgac ggccagacgc tccccttgag gctgcaaccg ggtgaccgat acaccgtatg
     9481 cggctgaagt acgtgtcacg gccgcagata aacctaacgg ctccccctgt tcaggcatac
     9541 ttgcctcccg tctctttatg ttgggtacct gttgtttcat ccactccact gtgttttttt
     9601 cacgggtaac tacagtccgc cacttatcct ctcgtatcag ccagggctgc cagaggacgt
     9661 aatagacaaa aacaacaacc agtaaggcaa cacatccgat aagcagcctg cgctcacggg
     9721 ggctaacata ctgcaaacgt tttttaagct cgttcattgt tcactctcag gatcagacga
     9781 ctttctaccc cctgcggctg ctgtcgtatc tcaccaggct ggatgaggta tttttctccg
     9841 gcgatctgct gaaagtgttc cagcgcctga aaagaagccg cctgcaaatc aattttcagt
     9901 gtctggctgc tgctgtccca ggcgagtgcc tgtaagcgta tcgttgaggt ttctgacagc
     9961 agttgctgaa gctggcgcat cacgctcccg agttccggta tattgttacc cgtcgactgt
    10021 tgcagatgtc tcagcatctg ccggcgggga tccttcaccg ccttttcact cggaaacagc
    10081 tgctgataga cacgtacgct ctcataccgc cagaattgag cctgctgcca gagccgataa
    10141 tgtgccagcc ccgcgttgct cccggccaga aaaacacagg ccagcagagc ccctgcgaca
    10201 tacctccatg ggcgaaatcg tgcataccat gttccctggc gggcaaattc tccctgccgc
    10261 agatctgcgc catcataccc gtcaccttcg gccaccaact gcagcagatc gcgtccgggc
    10321 tgatttcgcc actcagtggt cttcggaccc gggcacggta ctggcggcac ggagtaggac
    10381 tcaatcaccg gcggcgcagt aagagacaag aaatgtgtca gccacgataa ctctaccgtc
    10441 atcccggcgt aacgatcccg gcgaaacagc cactgcccgg caagctttac ggcactccat
    10501 ccttcagttg ccaacggcag catcagtacg tcaggcagca acctcacctc ccgaacccca
    10561 agttgtccac agtacgctaa ccagcgatgc ataaccgctt tttcgaccac cgccacatca
    10621 cacctgtcac ccgactgatg aagaatggcg aagtgcagct gctcaatgtc tgtcgccaac
    10681 tgctcttcca gaagaaaggg cagcacctgc aaacgctgac gccacgtgcg gtgtggcagc
    10741 gtgacctgat ggaaggcaat tctgcctgac ggaaccagca cccaggcggg ataacgggac
    10801 agcaaaccga ctaactgcgg atccgtttct ccgactgcca cactgccggc tcctgtctcc
    10861 tctggcgcaa tcagcctcca caataaaggc tccgagtcat cctccggcag tcgcagaaaa
    10921 agccgaccag cctcgacatg tttgttattt tttttcaggg aacctccctc atcgttaatc
    10981 catactgtcg ctgaatgatc cggaaacgct cctccctcca ctgcattaaa gtctgctgac
    11041 tgaacaggtg ttccccggag cgcacatgta accgaaccag aaaatagttg ctgctgacgg
    11101 ccaataccgg catggccgca ctggtgtcaa tgtccttaag cggtggcgcg tcaagaaacg
    11161 ccgccacgct ctcccaacca gtacggggtc gccgctgcag taattccgtc accggaagac
    11221 tgtccggttt cgtcagaaag agcgccgcca gcagcggcgc ctgtgaatcc agcagagtat
    11281 tcacattcac agacagatct gacgtaggca gtacacaaac atacggcaaa agtttgcggt
    11341 aaagcctggc atctattccc cggataaggc gtaactcact gacgtcctgc atcggctggt
    11401 tggctgccag atagggaggt tccatcccca tatacacctc gtcttcagca ccgcccctta
    11461 ccggcttatc atcacgatcg atccagtcac gtagggctgc cgtcacttgc cgggcctgta
    11521 aaagctctac ctgcaagttg attaatagct gctgaaaaat tctggcggca taaggtatac
    11581 tggtcagatc caccactcca taattaatag cattaagatt aaaacaggcc tgcgcatcca
    11641 cgatctgccc ccgcacatct ccccctctgg tctcaaactg tcgttctgac tgcgcccaat
    11701 tttgtgccag atgcgttttc cggggcgact cctgcctgct cctctgtaag atttgttttg
    11761 ctatcgtctc tgccgtatag ccatcccact ttgcctgcag ctgatccaac tgcgctactg
    11821 tacgcaaaaa tgtccggccg ttgcgctccg caatcaccgc cgctatcgtt accattaacg
    11881 acaggatcag caataccacc agaagcgcca cgccctgttc ccgcaatttc attcagcggt
    11941 ctccttgctc acaagaaaca gacggggcag ttcagcaaaa tcatccataa ccagcgtcac
    12001 ttcaacagcc tgcggcagga tgccggtact gtcccagcgc gcctgccaat caccgttaac
    12061 aaaaaaacgc agacgaaatg cgtgaacgcc gtccagcatc accctgacat ccggagaaac
    12121 accggaaggg tgatcgacat gaaagtagct caggcgttct aacttttgct gtcgcaaccg
    12181 ataacctacc cactgtaact ccgggcgcgg cagcatcccg gcgggattca gccagctgtt
    12241 tcgcgtaaaa gaaatccccc agtcatcact ttttagcaga tgaggtgcag cgagcagcag
    12301 gccttcattc ccccggctgt gccttgggag catctgcgta agatctcgct ccatctggct
    12361 gatccctctc tgcagctcag ccagacgctg tactttatcc cgggacagct cgtgcgcccg
    12421 tattccaccc tgcagcacct gaaatgcgct gatgctcaac gctgcaaaaa cagccagtgc
    12481 caacagcatt tccagtagcg taaaaccttt tacccgttgc tgactcattc acgaaccaca
    12541 ctgctacgca aggaaaccaa ggcggacgtc tcctctttag tgcgccgtac ctctacctct
    12601 aaggagcgcg gctgcggcac gtcgctgtcc ggcccgcccc agcgtaaata ccattcggtg
    12661 ccagcgaagc tgatcgtctt ctcgccccag ccattttccg gccaagtttt ctcgagttgc
    12721 aattgcacca tctggttatc ggccaaccag cctgccagca ccttctcttc catccgtccg
    12781 atccccgcag cctgctgggt cgaggcctgc atcagcgcca ttcctgccag cgcaaacaca
    12841 accagagcta ctatgacttc cagtaatgtc attccttctt gccccttcac aatgagacct
    12901 caccttcaaa aagagtcatc aaaccgttct catcaacctg cagcccgaca acggcctcct
    12961 ctccgacctg cctgaaaaga agtctgaacg gcgttacctc acctccggga agcagcaaaa
    13021 tatcgggttc agccccgtca tcatgctcag acatccactg caaacctctg agatactgca
    13081 gctcaagacg cacatcatcc ggaagtttgc cccccatggc tgcacgtcgc ggttgccaag
    13141 tttgccagac atacccctgc caaatatccc ctccctcagc taatgtcggg gggctttccg
    13201 ctgttccgcg ctgcagaacc ttaaatttcc atccgtcaaa ccgaacctga atccccatca
    13261 gcaagccgtc ctgctggctt ctatccaagg cataatccag ctgcgcctgc agacgctccc
    13321 gctgttgttg cagatgattc tgagcagagt caggaaatga cattagtacc agccctgcag
    13381 tgatgcccat caacaaaagg atcagcatca tctccagtat tgtgaaaccg cgctgactca
    13441 tggctgcgcg ttaccgagcg tccagttacc aatatcatct tcagtgtttg gcacaccatc
    13501 cgggccagga gaaaagatat caatgtcgct gtactgacca ggatttaaca gctgataatc
    13561 ccccccccaa ggatcctggg gcaaacggcg gatataacca tcctgccggt agttacgggg
    13621 ttcaggttgc acggtgggtt tactgaccag tgcccgcagt ccctgctccg ttgtcggata
    13681 ccggttatta tctaaccggt acatatcgag cgtgctttcc agcgcaacca agtcgctcat
    13741 cactttctgc cgatcggcct tatccttatt tcccatcaga ttcgggacca ccagactggc
    13801 caaaactccc aagatcacaa ttaccaccat tatttccagc aaggtaaaac cacgctgatg
    13861 ctgttttcgc atgtttttct ccgttgactg ttaaagaagg attcacatat tcatcatgtt
    13921 gttgagctgc aggagcggct gcagaattgc cagaacaata aacaaaacga ccccggccat
    13981 acccaccacc aacagcggtt caaacaatcc cagcgccagt tgcatctgtg tgctgaactc
    14041 acgatcctga ttgtcggcgg cccgttccaa catgctgtcg agttcgccac tattttcacc
    14101 gctggcaatc atgtgccgca tcataggcgg aaacagcgaa gtctgctcca gtgcctgatg
    14161 caggctgacc ccttccctaa ccaactcggc cgcggtagct aactgatgac gggcccagtc
    14221 attactcagt acgtcaccac tgatgtgcat cgcctggagc agcggtaccg cactggcatt
    14281 aagaatgctc agcgtgcggg cataacgtgc ggtattcaga ccgcgcgata tgcgccccac
    14341 gaccggcagc cgcaacagca gatgatgaaa agccagacgc tgggagggct gatgtaatat
    14401 catgcgcccc gctatcccgc ccagcgctgc ggccagtaac agccacgggc cccaagtctg
    14461 gctcacctca gcagccccca tcagtacgcg ggtagataag ggaagggtct gtttcatatg
    14521 aataaactgc tcaaccactt tcggcacaac cgcagtaagc agaatggcaa taaccccaac
    14581 cgccaccaac gtcagaacac aaggatagag caatgcctgg agtagacggt ttcgcatgat
    14641 ctggcgctgc tcggtatagt cagccagacg actgagcacc acgtccaaac gaccggatgt
    14701 ttcccccgcc gccaccatcg cacaataaag acgctcaaac gtacctggaa acatgcccat
    14761 cgccgcggcc aaggaatgtc cctcgagaac cttggtgcgc acggcagcga ttaagttccg
    14821 ctgacgcggt ttttcactct gcctcaacaa cgcatccagc gcctcttcaa gcggcagaga
    14881 tgcggccact agtgtggcta actgccgtgt cagtaacgcg agttcggcgc tgctcccccc
    14941 gcgcggacga aacctcagga tactccgcag tgagtgcttg tgatggacct gtttctcatc
    15001 caacctgact ggaataagcg cctgctcacg cagcaactga cgggcatgtc gggccgaatc
    15061 cgcctccaga atgccactgc gttttctacc gtgaatatct gatgcctgat aatgaaacaa
    15121 cgccatatca ttcctcttcc tgcaatctgc tttcagtaac gcgaaccacc tcctcccaac
    15181 tggtctcacc ggctagtacc ttttgtcgcc cgtcgccacg cagcgtcaga tagcgactcc
    15241 ctgccaagcg ggtaataccc agctccccct ccccctgata gattgccgtc cgaatgcgat
    15301 cgtcaatcag caacagctcg tggatcccgg tgcgcccgtg atagcccgtg aaactgcagt
    15361 gctgacaccc ggcaggctgc cagatcgtgg tttcaggggg aatgtccatg tccatcgcta
    15421 actcagtact gacctgacag ggctgacgac agcgcggaca caacgtgcga accagtcgct
    15481 gggccagaac agccagtaat gaggtggaaa gcagaaaagg agggatgccc atatcctgca
    15541 gacgcgacag cgcgcctgct gcactattcg tatgtaacgt tgacagcacc aaatgccctg
    15601 tcagcgaggc ctgcacagca atctgggctg tttcaccgtc acggatctcc cccacaagca
    15661 ccacgtccgg atcctgacgt aaaatagcgc gcagactccg ggcaaaggtc atgtccacct
    15721 tcgggttgac ctgggtctga ctgataccgt ccagttcata ttcaacagga tcctcgaccg
    15781 tcataatgtt gcggtctcgg ggattcagca ggcttaacgc ggcataaagc gtcgtgcttt
    15841 tacccgaccc tgtaggcccg gtcaccaaaa taatgccgtg aggacggcgg atcagtgcat
    15901 caatgtgtcg ctgattctgc tgcgacatgc cgagtgcagc aagatcgaga ctaacgctat
    15961 ttttgtctag taaacgcagc acgatgcgtt caccatgatt tgaaggcaac gttgaaacac
    16021 gcacatccac cgcccgacca ccgatacgca gtaccatgcg gccatcttgg ggaacgcgtt
    16081 tttcggcaat atccaaccgg gccatgactt taatgcgtga aatcagtaac gaagccagat
    16141 tcctatgtaa acggaggatc tcccgcagaa cgccatcaat gcgaaagcga acctgcagat
    16201 ggcgttcgta agtttcaata tgaatatctg aggctttctc tttaattgcc tctgtcagca
    16261 ttgcgttaat caggcggata attggcgcat cgtcattcgc atccagtaag tcctcacggt
    16321 caggcaactc ccctgcgacg gtataaaaat ccatttcatt accaatctca gccatcattt
    16381 gtcgcgcttc atcagaatca cgttgatagc tgctcaccag ctgtttttca aacgcttccg
    16441 ccgacactgt tgtgacattc agaggacctt cagccaccct gcgcacctcc aacagggcac
    16501 tggcaggtgt gtcctccatg cagaacactc gattcccctc ctgttcacgc agcaacaaaa
    16561 ttctctgcgt acgggaaaag gaaaaaggca gaaggggacg tgattcactg acattctgta
    16621 ctactctgct cattacatct cctgcgcata aaacgcagca atcgccgctt tcaccttccg
    16681 gaaagccgta tcgtcttgat cgggggacag cctgagctgt tcctcattca gcgtcagcgc
    16741 cgttgtttct ttgccacgcg actgcacctg ttcctgatta aacgactggt atttttcggc
    16801 cgaagcatga cgaaatccgt cgcgctcacg aataatagtt ggccgaatga acagcatcag
    16861 attacgctta gctgtctgtt cgctttttgc gcgaaaaaga tgccccagta ccgggatatc
    16921 ccccagcagt ggcactttac tctcactccc cctgatcgac ttatccagta atcctccgac
    16981 caccaccgtt tcgccattcc cgaccagcat ggcattggtc actgttcggg tgttgaaggt
    17041 tgctcccaag tcagtagtgg tggctactgc agtgtccgcc acaccggaca cctcctgttc
    17101 tatctccagt aacacggaat caccctcgtt gatctggggt tttaccctga gtttgatccc
    17161 caccgttttg cgctcgaccg tgttaaaaat attgtcccca gaggttgtct gtgagcccga
    17221 aagtaccggg acctcctgac caacattgaa agtggcctcc atattgtcca gcgtgacaat
    17281 actgggggtc gccaagatat cattcttgct acttgtggag agcgccgtca acagcatcgc
    17341 ccagtttccc tgataaaacc ccgctgcaat tccgttaaac atgctcaggg cattgctctg
    17401 atcgctgtct aagatttcgt tctgacgagt ctgaaccatc gtggtaatag gtaaaccggt
    17461 actggtgaac tgcgccacac cggcatgttt attcacccac tggatcccaa ggttcagccc
    17521 gtcagcatcc tgtatttcag caatgatggc ctccaccagg acctgaggac gacgaatatc
    17581 caactgcgaa atcacatttt caaggtcgcg gatgacgtca ggggcccccg tcaggatcag
    17641 ggcattggtt tgttcgtggg atttaatcgt cactcctttg cgcaaggctt ccgttgacgg
    17701 actcttttta gagtcgtttt caatactgct gctgatacct gtcaggactt ccactaaatc
    17761 cttcgccttc gcatatttca ggtaaatcac cttagtatta ccctgaacat cctcctgacg
    17821 atccagttgc ttaattatgg agatgatacg ctgttgggag attggctctc cgctgacgag
    17881 cactgagttt gtcctctcgt ctgcaaccag tttcgccgtc atccaagcag gtatagctgt
    17941 cttatctgtt tctttagtca gttccgtaac aagtctggct acgtctgttg cggacgcgta
    18001 ggtgagcggg accgtggcaa cgctgcgatt acccacctta tccacacgtt caacaatctc
    18061 catcaaccgt ttcatcactg cagcacgtcc ggtcatcaac aaaacattag aaggttcata
    18121 atgcaccacg cttcctgcgc cagcattatc attgagctga cgcagcaaag gcgccagatc
    18181 tctggctgcc acgttactta cggggaccac ccgggtaaca acctcatcac cagtcccggg
    18241 actgacatca ctagctaccg gcaccgccga cgttttggca tcttttgagc gcactacttt
    18301 cagtataccg ttgtgcatat cgaccacagc aaaaccataa acgtccagca cactgagaaa
    18361 gaactgataa tattgttcct cgttcagcat atcgtagctg cgtacagtga tggttccctg
    18421 cacgtcagga ttaattatta ccgttttgtg taaattttta ctgacagtat ttatgaactc
    18481 ctgaatatcc gtatctttaa agttggccga aaattcagcc ccccaccctg gaatggcccc
    18541 caaaattact gtagtgaaca caagtaccgg caaacgccca ttcaaccatt tcctgaacaa
    18601 tcagttttct cctacagcaa taaatgcatc atataactga ccatcacgct cgacggttat
    18661 ttttatttct ttaagttctg gcaattgctt cattatctgc tgcgcctgtc tggtatcacg
    18721 caattctgaa ccattaaccg atacggccag atcgttatca tgcaacccag cattataaaa
    18781 taacgaactg tgcttacccg gaatcaagcg ataaccgctt aatgtattat taaccgtaac
    18841 ggctgaaaag ctcataatat caaaaacact ttctacccga aaattatctt gggttatcac
    18901 gtctccaact gttaaattat tattgtcata tgcattcttt ttttcggtat tatcatatcg
    18961 cagaggcagt gatacagttt ttccctgata gttaataaca atatgatcgc taaaaatagc
    19021 actaatcgta gcatcgtagc tgggtacttt ttcgccgacg ctcagactga actgtctgtt
    19081 attatgtgca aatatggcaa cggaatgctc tgcaaccgaa ctataaagta tacctctcac
    19141 gtcaagatcc tcccctgaaa gcagatcatc tgacagggaa ggcattgttt cctgcgctct
    19201 cccctccgat gatagtgggg atgccctgcc gaataacgta aaaacaaaac gttcttcagt
    19261 ttgatcacca gttaccgctg tttccggcgc gctaacacgg gataagccgg gcaccgatgc
    19321 tgcctgagtc agccagaaat gatggataac cgacaccagc tgatatccgg caataagtaa
    19381 aattacgcac aatagccggt ttggcgtcag cattttaagt acgatatctt ttataaataa
    19441 gccccggtca ccccggaatg atagaaagaa caacataata aaacgctgga cgctacttta
    19501 tgttttgatt aacgggacga atgtgttact aatgcggccg aagtggccgc accgtctcag
    19561 gattatttat atacaaccct cattgaccta ggtttactga agtccaaata ctgtccgttc
    19621 caggctttaa cttttttcat atcagctggg ttcacattac ctacaaactc accaatcctg
    19681 ttataggaca ggttatccca gtcagaaagc agcactttcg ctttagcccc gctgtcgaca
    19741 tagacttcct gcccaacaat ccagtcagga aggctatatc ctgaccgttg accaaccggc
    19801 aaacagtaac gtttacctga attcacggac acgatgcatc cctcgttttc ttttgctgga
    19861 agtgcctgcc catttacagt ataggtaagt ccttctggcg caggtgtgag cgatttggta
    19921 tccagtatct tgttattgca aaccaatgtg gcctgtgtgg gctgactttc tgttggcacg
    19981 ttaatatgga acttattcat tacagtgttg ttagcccggt gattagccag tctgaatcgc
    20041 aactgccctt ctttcgtatc cacctgcagc tggcagtcgt tatcggatag attctgacta
    20101 tcatcggaat aagtgaagcc ataggcacca tacatcgcag gatagatgta gcttgacagc
    20161 gtgccttccg gatcgtaata ccccaccagg gtcgtcacag gaacaccaaa ctgctctggc
    20221 ttacgcgctt cacgagtatc aaccacatca cgttctttcc agaactgacc atcggaaaca
    20281 aagctctttt tatacccctg ggaaacgacc ttttcgtcac catttataaa cagataacta
    20341 ttataaccgg cctcatggtt gatggtcagg atactgcctc tattatctgc ggaggctgta
    20401 gggatataga tgtttcttgt ccagttaccg ttccacatat gcactttgac gacagcgtac
    20461 tctgccatca gctcagccat tttgctttca cttagctcat tgactgaagc cgtaatctgc
    20521 tccgcacggt caatggtgtg ttcatacggt tccatttcct gcgtatctgc attccacttg
    20581 ctgaagccgg tggaggaacg gctatcgaac acagctttat tttcaaaaaa acgctggatg
    20641 atagccgatg aattcggagt atacattgtg aaacggtttg cagcagagaa agggctgcct
    20701 cccgccatgg cgtcaaaacc aaatttgtgt ccatcaaacg gttcttgaca ctgattattc
    20761 agacaactct tttcatttgt ttgagacgga taaaagttag gaataaaccg ttttttatca
    20821 ctatcccatc cccaagttga gttgttattt tctgcactac gatgtacaga acccttgaaa
    20881 ccatctacat aatgaccaag accataatta tgaccaactt catgactgaa ctcattcccc
    20941 aatgtggaat ctaaagtaac aattccccca cctccggagc caccatgaac ctggatgcca
    21001 ttagcataat taccgcggct attatgtgcc gctaattgcg caactacata tggatgacta
    21061 ttctccccta agcctgcggt actatttaaa ccatagttag cattatcaat gccatgcgaa
    21121 accaattctt tacctattct ttgacgcatt gtaccactat gccacccacc atttcctgga
    21181 tccatatctg tcaataactc tccggttggt aacataactt cctttaggtg tagaggcgca
    21241 taattattaa caatcatacg acttacagga atggtctgga aatattccct atgtgcttct
    21301 ttgtctttgg caaaatcaaa gcgatcccgg ggagtggtca acatcccgat atcaattgta
    21361 tgcaacaaca gctcacccgg tgctccaatc ttgatatcat ttaggcgacc gctcagattg
    21421 ccctgtttaa tcaccaagtt taaaccaggc acgatccagt gcgcaggcag ttcagcactc
    21481 caaatatgct gagcataagt gattcgatta ttctccagtt caccggagcg gaaccactga
    21541 ccatttacat atttgaacag aagagtgtta cccaccgaga gtgtgacttt tcgatcacca
    21601 taaaaaaccg ttgaactata gcctgcagac gaaacaaagc gaaccatctt accttcaagg
    21661 tcgggtccct gcggcagata aatgtctctt acccaacgac catttgcagt atggatctcc
    21721 accagtgcat tatttgttag atggctatga atagaactcc cgctggcatc actgagtttg
    21781 tttacttcag ccaccgtatt aatgatcttt ttcgttccat tatgaggtgt gaaatcgata
    21841 ccaccttccg gaacaccatc cagatggtag attgtatccg gtagtgatga aggaggataa
    21901 agggttaacg taccgagaat tttattatta tcatcgcggg cttccacctg aacaggtgtt
    21961 ttatcatcag ctttcaccgg acggacaagc agcagacttt tccgcaggct ggtcagatgt
    22021 ggttgactat ccccttcttt aggatgggcg ggtaaaatct ggctttgtgc aaatttcacc
    22081 tctgcggcca acgaaccctg cagatcattt ataggctggg aggtattgaa ataaatggct
    22141 gaattattat cagcagctgt tgcagaaaat accccaatcg ccagaggggc aaggatcgta
    22201 catgacagat actttaattt catcggtgtt ctccatctct cattcatttt agtgttcatg
    22261 cagccggata taacagtatt tctatatttt attttttcag gagcgactta ttcactagtc
    22321 gttggttatt tttataacat atttacacct gtttcgtaaa tatatcctga ccaaagaatt
    22381 aactcttata acgtccttta acttatttta ttattatatt ttgcaaaaaa aagcaagcta
    22441 actatccttt tgataagcaa gcacaaaaca cgacaattaa tatgtttata acgtttaaca
    22501 catcaaacaa ttgcattatg atacattctt ttgtgtgata atgaatcaac aacaaatatt
    22561 taatatacaa aacaaaactc aataaaaaat aaaaaccaaa aaaaataaag gtattcttag
    22621 ccactatatc cagattgaat cgccacgggt ttaacagaca cctcagagtc attttaaatg
    22681 gcttaaagag aggtgcccat gagcggtaag cgttatcccg aagattttaa aattgaagcg
    22741 gtaaaacaag ttgttgaccg cggtcattct gcttccagcg ttgcaacact tcgccatgct
    22801 gaggcctgat aacatattcc tgggtaagct tccatggcgc ataacactcg cttacgttga
    22861 actgaccgct cagtaaatat caatacagtt ctgacgagcc gcttacaagc tgtgattcat
    22921 acagactgag cacacgagct gcgacagcaa caccgatgcg ttcagcaagc ttcagggctt
    22981 cactgcgaaa ttcaggcgaa tgctatttac ggggtttttt actggttggt actgtttttt
    23041 tgtcatatga gccacctctg actgagagtt tactcactta gcgtgtgtcc actattgctg
    23101 ggtaagctca ttatcctgaa aaagctgcct cagtaaggat cctcttcccc ttctttacgc
    23161 gttttcagca acttgaggat ccacgtatac tgctcaggat gtggacgaac gaaattttca
    23221 accacttcgt ttatctgtcg cgcaatatga gcatcactct tctgtcgtat agcaagtggc
    23281 gggctgacat gaatagtcag atgatgggtc tttccgtcat aaacagggaa aagcggtatt
    23341 atccgtgcac cactgatcct gctgagccgt ccgataaccg gtaaagttgc tttatacgtc
    23401 gcaaaaaaat ctgcaaactc actgaattcc gggccatgat cctgatcagg aagataatat
    23461 ccccagtatc cctgccggac tgagcggaca aaggaggcaa taccgtcatt acgtgcgtgc
    23521 agttttcctc cgaaacgacg acgaacactg ttccagacat aatccaccac cggatttctc
    23581 tggttatgaa acatcgctgc catttttctg cccgacgcag ccatcagcat tgcgggaata
    23641 tccactcccc aggcatgtgg cacaagaaaa atgacctttt cattgttctg tgccatcttc
    23701 tccacaatct ccagaccatt ccagcgtata cgatgcgaaa ttttatcagg cccggacagt
    23761 gctaattcag ccataagcac aacggccatg gaggctgttg caaacatggc atcaacgata
    23821 ttctcctttt ctttatcact gtactccgga aaacaaagcg ataaattaat caaggcccgt
    23881 tgacgtgcgc ttttccccag acgcccaacc agcatgccca attttcccaa caatggatct
    23941 ctcagtgcgg gggggaccat tgcaaacaca cagataatac cgatacctgt ccacgtcatc
    24001 cagtaacggg ggaagcataa tgtaaggtta aagcggggaa taaactcagt tctgatattt
    24061 ttcatattta ccggtatctg cctgcttatc tcattatgat aacagagtac agcagaaggt
    24121 ggtaccatcc ctggaggcca ccggacagca gaggaaaaca catcctcctg ccttcagaac
    24181 ggaaccgaag ataagaaaat actatggctc accatacgga agagtgtcga aatcagtgag
    24241 cccattcttt ttatacggat ttccaatcca gcgggtggtt tccctgaatt gcggattcac
    24301 cagagaacgg gtcggatcgt aaccatcgta tgtcagtccc gccaaatctg accaggtgtg
    24361 gatcagttca gccagactgt atttgcggtc aacatactgc gaaaaatctc gcggatgtgc
    24421 agcatgccac ttttccgagg tccacagcag gaacggaaca gtgtacatgg gacgtgtggg
    24481 gttatcttca ttccgtccct gcgttttata tggcggagta tcatatactt cttcaccatg
    24541 gtctgaaaaa taaagcagaa agccgtccgg cgcagttgct ctgaagtcct ttatcagact
    24601 ggccaccaca tgatcgttaa acagattggc attatcgtaa tcgttataca cttccagctc
    24661 tttcgcattt aatccagtgg gaatatgccc tgtaatgcca tcaaaccgcc cctgaccttc
    24721 cggataacgg tatttatact taatgtgcgt acccagcaga tgcacgatga tcagtttctt
    24781 tggtgcaggg tcattcagca cttcccggaa cggcttcagc acgttagtgt catattcacg
    24841 cgcactttgt gttcgctgct gattcatgta gtactgcctg tccgtctggc gcgaaaatac
    24901 agtgagcatg gtgttacggg ctgtgattgt ctgctggttg gtaatccaga atgttttata
    24961 gcctgcctgc ttcatcatgt tcatcagcga cggctgcgtc agatacagat caggattctt
    25021 ttcgttggcg aaagtaaggg cctgttgcaa tgcttcaatg gtgtacggac gcgatgccac
    25081 cacattatta aacacagtaa gacccggatc ggttttacgc agtgcatcca gctccggcgt
    25141 cgtttcacgt agatacccgt ataagctcat gcgttcgcgc tgtgtcgact caccaatcac
    25201 caggaccagt gtgcgtggtc tctcccctga ttcatccctg agattaccca gtggtggcag
    25261 tgcgctattt tcattcagga aggttgtcag tgcattcagt tgctgatggt actgataata
    25321 actggataca aactgccagg gggcagcagg ctccattcgg gatgccagtt tgcctagagt
    25381 atcattcagc ggctcctgtc tgattaacga tttcagaaca accggatgca gaagcagagc
    25441 ataaagcagc aggaaagaga caattctccg ccatggcaaa ggaatatata caggacgcag
    25501 acgtgtccac agaaaaacgg acaccgcagt atataccagc gagataagca acagtttaag
    25561 gctgaaatac tggctgaaat attcaccagc ctctctggca ttcgtttcaa acataacgaa
    25621 aagaacactt tgagagaatt catgaccata gagaaaataa taacaaagtg ctgccagcga
    25681 cgtgccccag agaatgaaac cgacaacagc tgcaattatt tttatccgat caggatagag
    25741 aaacaccggg atcaaccaca gacaactgaa taacagtgag tcccgtattc cattcgttcc
    25801 gctgtaccca ctgctgaaga tgatgacctg caggagagtg gaaaaaaagc caaaataaaa
    25861 aattgcccat cccagcgcgc tccagctgaa agtaggcctg ttctgtccgg tatttaaatg
    25921 cattgaccgt ccccgtattt aaacaatgtg ataaattact ccgttaccgg aaaaccgctg
    25981 aacaaaattc gggctgaaaa gaggatccgc cgttatctgt tgcatttccc cttagcctga
    26041 ctagccagag acacaatgat ctgtgccgtt ctgttaatat caaaccggta ctcaatatct
    26101 tctctggcgc tggctgccat catccggaag cgttccggtc gggataaaaa atcgcgcagt
    26161 gcgccggtcc atgcagacac atcccccacg ggtaacagcg tccctgtcac attcttctga
    26221 atgacatcag ggatcccgcc cgtctcactg gcgataacgg gcacgccgga gactgacgct
    26281 tcagccagta ccataccaaa cgcttcattt tccgaaggca tgaccaccac actggcaatc
    26341 cggtagaccg gtaacgctgg gaaaagggca cctgccatta acacatctcc gctcattccc
    26401 aggtgttctg tctgctgacg cagacgtgct tcgtattctt cacgcccggc gcccaccacg
    26461 agccagcgaa atgatttccc ttccatcttc agctgataca atacacgcag cataaattca
    26521 tgtccttttt cgggacgtag catccccacc tgaacgataa gcggaacatt gtctgctgat
    26581 gcagcccagg cgtggatatg caggggtaac ggtcgcatgg cttcattatg caatgcgggc
    26641 cagtcgaaac ccggtggaat aaccgttacc ggtgtcctga caccttccgc catcagatgc
    26701 gccatcatgg ctgagctagg cacaacaatg aaatcacaca gataattcag ggaaaacgtt
    26761 ctggtcttac gggtgatgta ggttttttgt ctgacaatac tgaagcggtg acagcatatc
    26821 agacggctca gtcctgctat attactgtca tggccactat ggcagatgac cagatcaggt
    26881 ttaaattccc cgataatccg tcgaagtctg aggatggaag gaaggtgaag gctgttcctg
    26941 aaaggaataa aagtgacatc atgccctctt tttctggctt ccggagcaat tttacttttt
    27001 tctctgcagg caagtaaaac ggaatgtccc tgcttttgaa gagcagtcat ctgggccagt
    27061 gcctgcagct cctggccccc aatatctgac gaagattcag tgaataaaat tttcatcatt
    27121 aattatctgg taatcttggc cccttatgaa gagccagtac tgaagaaaaa agtggcgttg
    27181 tataataaaa aaggcgtcgt ttcagccagc cggaatgttc ccgctctttg gtgctgatac
    27241 gccctatgcg taatgaacca ttttcaggac agttcattct tctttcggtt gtatataaat
    27301 aagagaaacc aagcttaccg gcgagattaa tataatcccg attataatat ccttcaggcc
    27361 agcacagatg ggaactacag aaccccagct tttccgtcag acattgtttt cccaccagaa
    27421 tatcttcttt catgagccgg cactgttcag cacgtgatac agacaacctg tcccagcgtt
    27481 tatgtgaatg ggtgtgcaca tgaaattcaa ccagtccgga atcacgcatt tcccggactt
    27541 cagaccagcg aagcatgacc tcatctgaac ggttatcggc aatcaaccgt tcacagtcgc
    27601 ggtgagaata ttcctgctcc tgcctgctac gtacgtttcc cttaccaatc agccccgtaa
    27661 tgagaaaaat atgtgcatga aggttatatt ccttcaggac cggccatgct ctgagccagt
    27721 tatcaagata accatcatcg aatgtgagca tgacactctt tcgtggaagc gttcccccct
    27781 gataaaaata ttcaagctca gcagatgtta ccgtccgcca gttattatct gcgagccact
    27841 tcatctgttc acaaaatgtt tcaggtgaga gtgtcacaag ccccggacaa cgactgacat
    27901 gatgatacat cagtacggga agatgccttg cattcaacat aaaaaaataa tcgtctgtta
    27961 agcattctga aaatatcctc gcgtaaacaa cagtgaggat ccatagatat cacacatggt
    28021 gatattattg tgtaatcccg gaatggtccg gagattaccg acaacaggga ttttttaata
    28081 tttttatcag ttaatcaacc agaacttaaa tttcccttaa cgcatatgct ctttttaatc
    28141 agattttctg tttttcagaa aaaacagaat accgcaattt cagtaaacac agcgagagat
    28201 atccgcctca gtaaaaaatc agaagattat catcctgatt tattgcataa aaccagtcga
    28261 gtagcggaat tttctgcatc cggataccac tccccctgaa aaatactcgc acaatatcgc
    28321 gccgcatgca ctgatggacc gggccatcgt gagaatctct tgcatcgtag atattaacca
    28381 caccagtgcc ccccagaaca acagtaatac ctgtagcaga cctgccatag aggttaaata
    28441 accattcata tccctcttct acccattcat cccgaagttt cagccagcga cgaatggact
    28501 gtacctcctg tttcagccga ggggctgagt tgacaaaccg tttagagccg ccggatattc
    28561 aggttaccgg cctgcaaatc aatatcagac agttttatac ctaataactc tgatgcttgg
    28621 acaccatgaa gataccccat aaggagcagg cagtgattac gttccgggtg tggcactttg
    28681 aagacagcac tgtgcaactt atgaatctca cccgggttca gatatttttg ttgtccatta
    28741 cctgtcctta tttattgaaa gtcgatatta gtttaaaaag ctgctaatca tgacaccatt
    28801 a
//
LOCUS       DAZFBR010000058        28471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFBR010000058 DAZFBR010000000
VERSION     DAZFBR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.058"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1825
                     /gene="espX6"
                     /locus_tag="ACHJZ2_002718"
     CDS             <1..1825
                     /gene="espX6"
                     /locus_tag="ACHJZ2_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HFO4568799.1"
                     /translation="KDKDKDISHHCNVKTEQLQQFIKIMEQKAQLPIYIDKNNLKESI
                     FSVLHNDPQQVDKDQHLPCEKFLKHACKSSNSFEVKLDATHQYQHLNNFMISFDPVEN
                     QLTIRDNNNKTETFSFTNLQWENLLQYYKENHQQPNIAGSRNLTDNIDKIKNTISTSE
                     IIECASPEIRSSVLNDLYSIANFLPDNNLTPNESWKRFCETCERFYVAQKSITGDKSE
                     RLTRKLSISDAGITMTFKIGDVVINTISTAIPEDATGQRCIEGLNLAEMDLTDIDLSK
                     MALRNVNFNGSILRNAKFSGTICEGVDFTDCDLRNAEFENASLENNDFRKVRHLTYVN
                     FKNANLRNSNFNGKVLTGVTFTGSDLSNAYLEHIDFTTVILYETSKIPGIPGTPGTPQ
                     IPGTPKVILTGAILNYSDLSGKDLSEYNLTGILCMYTNFSNANLTNCKISNANFSNAK
                     FYNTNCTGANCSNILFDYAWFDNTIFIKTLFKNTCFYNVRAKNVYLEGAYLNNDNIVN
                     QANNSTEKQSIDSTDKQANDSTVQQSIDSTVQQANDSTDKQANDNIDKQVNDSTDKQA
                     KNSTEQQDSNSFNQARLKKEVNRRFSIPGLTSYQPTYIVEE"
     gene            2091..2933
                     /locus_tag="ACHJZ2_002719"
     CDS             2091..2933
                     /locus_tag="ACHJZ2_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO4568800.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            3001..4179
                     /gene="yjiJ"
                     /locus_tag="ACHJZ2_002720"
     CDS             3001..4179
                     /gene="yjiJ"
                     /locus_tag="ACHJZ2_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4568801.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            4390..9631
                     /gene="iatD"
                     /locus_tag="ACHJZ2_002721"
                     /pseudo
     CDS             4390..9631
                     /gene="iatD"
                     /locus_tag="ACHJZ2_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(9788..10383)
                     /gene="kptA"
                     /locus_tag="ACHJZ2_002722"
                     /pseudo
     CDS             complement(9788..10383)
                     /gene="kptA"
                     /locus_tag="ACHJZ2_002722"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            10588..11271
                     /gene="yjiH"
                     /locus_tag="ACHJZ2_002723"
     CDS             10588..11271
                     /gene="yjiH"
                     /locus_tag="ACHJZ2_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO4568802.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            11268..11729
                     /gene="yjiG"
                     /locus_tag="ACHJZ2_002724"
     CDS             11268..11729
                     /gene="yjiG"
                     /locus_tag="ACHJZ2_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO4568803.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            11742..12914
                     /gene="iadA"
                     /locus_tag="ACHJZ2_002725"
     CDS             11742..12914
                     /gene="iadA"
                     /locus_tag="ACHJZ2_002725"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO4568804.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            13036..13890
                     /gene="hypT"
                     /locus_tag="ACHJZ2_002726"
                     /gene_synonym="qseD"
     CDS             13036..13890
                     /gene="hypT"
                     /locus_tag="ACHJZ2_002726"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO4568805.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(13883..14275)
                     /gene="iraD"
                     /locus_tag="ACHJZ2_002727"
     CDS             complement(13883..14275)
                     /gene="iraD"
                     /locus_tag="ACHJZ2_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO4568806.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(14272..14355)
                     /gene="idlP"
                     /locus_tag="ACHJZ2_002728"
     CDS             complement(14272..14355)
                     /gene="idlP"
                     /locus_tag="ACHJZ2_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO4568807.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(14471..14614)
                     /gene="ytiD"
                     /locus_tag="ACHJZ2_002729"
     CDS             complement(14471..14614)
                     /gene="ytiD"
                     /locus_tag="ACHJZ2_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO4568808.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
     gene            14948..15778
                     /gene="yjiC"
                     /locus_tag="ACHJZ2_002730"
     CDS             14948..15778
                     /gene="yjiC"
                     /locus_tag="ACHJZ2_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO4568809.1"
                     /translation="MSMPLSNVLQSQIITDNHFLHHPKVESELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(15919..16692)
                     /gene="uxuR"
                     /locus_tag="ACHJZ2_002731"
     CDS             complement(15919..16692)
                     /gene="uxuR"
                     /locus_tag="ACHJZ2_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO4568810.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(16907..18367)
                     /gene="uxuB"
                     /locus_tag="ACHJZ2_002732"
     CDS             complement(16907..18367)
                     /gene="uxuB"
                     /locus_tag="ACHJZ2_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO4568811.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(18448..19632)
                     /gene="uxuA"
                     /locus_tag="ACHJZ2_002733"
     CDS             complement(18448..19632)
                     /gene="uxuA"
                     /locus_tag="ACHJZ2_002733"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO4568812.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            19972..21315
                     /gene="gntP"
                     /locus_tag="ACHJZ2_002734"
     CDS             19972..21315
                     /gene="gntP"
                     /locus_tag="ACHJZ2_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO4568813.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(21489..22391)
                     /gene="fimH"
                     /locus_tag="ACHJZ2_002735"
     CDS             complement(21489..22391)
                     /gene="fimH"
                     /locus_tag="ACHJZ2_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO4568814.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(22411..22914)
                     /gene="fimG"
                     /locus_tag="ACHJZ2_002736"
     CDS             complement(22411..22914)
                     /gene="fimG"
                     /locus_tag="ACHJZ2_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO4568815.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(22927..23457)
                     /gene="fimF"
                     /locus_tag="ACHJZ2_002737"
     CDS             complement(22927..23457)
                     /gene="fimF"
                     /locus_tag="ACHJZ2_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO4568816.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTPLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(23467..26103)
                     /gene="fimD"
                     /locus_tag="ACHJZ2_002738"
     CDS             complement(23467..26103)
                     /gene="fimD"
                     /locus_tag="ACHJZ2_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO4568817.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLARNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLNNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(26170..26895)
                     /gene="fimC"
                     /locus_tag="ACHJZ2_002739"
     CDS             complement(26170..26895)
                     /gene="fimC"
                     /locus_tag="ACHJZ2_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO4568818.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(26932..27471)
                     /gene="fimI"
                     /locus_tag="ACHJZ2_002740"
     CDS             complement(26932..27471)
                     /gene="fimI"
                     /locus_tag="ACHJZ2_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4568819.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(27536..28084)
                     /gene="fimA"
                     /locus_tag="ACHJZ2_002741"
     CDS             complement(27536..28084)
                     /gene="fimA"
                     /locus_tag="ACHJZ2_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4568820.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         7387 a         7085 c         6739 g         7260 t
ORIGIN      
        1 taaagataaa gataaagata taagtcatca ctgcaatgtt aaaaccgagc aactgcagca
       61 gtttattaaa ataatggaac aaaaagcgca actaccaatc tatattgaca agaacaattt
      121 gaaagagagt attttctctg ttttgcacaa tgacccacaa caagtagata aagatcaaca
      181 ccttccctgt gaaaagtttt taaaacatgc ctgcaaaagt tcaaattcat ttgaagtgaa
      241 attagatgcc actcatcaat atcaacacct gaataacttc atgatttctt ttgacccagt
      301 agaaaatcaa ttaaccatac gggataacaa taacaagact gaaactttct cgtttacaaa
      361 cttacaatgg gaaaatttgc tgcaatacta caaagaaaac caccagcagc caaatatagc
      421 aggatcacga aatctcacgg ataatataga taaaattaaa aatacaatat ccacctctga
      481 aattattgaa tgcgcctctc ctgaaataag aagtagcgtc ctgaacgatc tttatagcat
      541 tgctaatttc ctcccggaca ataatctgac cccaaatgag agctggaaaa gattttgtga
      601 gacatgcgag cgcttttacg ttgctcagaa gagtatcact ggagataaga gtgaacgtct
      661 tacgcgaaaa ctctctatct ctgatgcagg aattacaatg accttcaaga taggtgatgt
      721 tgtcatcaat actattagca ctgctattcc tgaagatgca acgggtcaac ggtgtatcga
      781 agggttgaat ttagcagaga tggatttaac cgacatagac ttgtcgaaaa tggcgctaag
      841 gaatgtcaat tttaatggca gcattcttag aaatgccaag ttctccggta cgatctgtga
      901 aggcgtggat tttaccgatt gtgatctgcg taatgcagaa ttcgaaaatg cctcattaga
      961 aaataatgat tttcgtaaag ttcgccactt gacttatgta aatttcaaaa acgcaaatct
     1021 acgaaacagt aacttcaacg gaaaagttct cactggcgta acctttactg gaagtgacct
     1081 tagtaacgcg tatcttgaac acatagattt cacaaccgtg attctatatg aaacatctaa
     1141 aatacctgga atacctggaa cacctggaac acctcaaata ccgggaacac ctaaagtaat
     1201 tcttactggc gcaatactaa attattccga tctatcggga aaagatcttt cagaatataa
     1261 tcttactggt attctctgca tgtataccaa cttttcaaac gctaatttaa caaattgtaa
     1321 aatctctaat gcaaactttt cgaatgcaaa attctacaat actaattgta ctggtgcaaa
     1381 ttgttcgaat atcctatttg actacgcatg gtttgacaat acaatattta taaaaacgct
     1441 ttttaaaaat acctgttttt acaatgtcag agcgaaaaat gtctatcttg agggagcata
     1501 tctgaacaat gataatatcg tgaatcaagc caataacagt accgagaaac aatccattga
     1561 cagtaccgat aaacaggcca atgacagtac ggtgcaacaa tccattgaca gtacggtgca
     1621 acaagccaat gacagtaccg ataaacaagc caatgacaat atcgataaac aggtcaatga
     1681 cagtaccgat aaacaagcca agaacagtac cgagcaacag gacagtaaca gttttaatca
     1741 agcccgttta aagaaagaag tgaataggag attttccatt ccgggtttaa cgtcttatca
     1801 gccaacatat atagttgaag aatagttcat taatcaccca aacagagtgg gtatggcaga
     1861 cgatatagat taacatttaa acgttaaccg cctcatctat cattcataga tgaggcgcat
     1921 ttatcagtcg atgtaagctg aaaatgttct gccatccaga gacaaccgcc gccccctgcg
     1981 gcgtttacta tttcatcgcc cgcgttaaga attcattaag cttcatccgt atactcctta
     2041 tctgtcgcac tgaatttgac gagaaaaaag caataataag gagcaataag ttgaaaaagc
     2101 gcatcacctt tatcgttttt ctttgcgcca tcgtggcggc aagcctgttt tttgtgcaat
     2161 cctgcgtccg taaaagccag catgtcgcag ggtttcagaa ttatcaggcc actatcgatg
     2221 gtaaagagat tacaggagta actaaaaata tctcctcact gacctggtca gcgcagagca
     2281 ataccttatt tagcactatc aacaaacctg caaccatcgt tgaaatgacg accgagggcg
     2341 acttgatccg caccattccg ctcgattttg ttaaggatct cgagacgatt gaatacattg
     2401 gtgataataa atttgtcatc agtgatgaac gtgactacgc catttatgtg atttcactta
     2461 acgctgactc ggaagtgagc atcctcaaaa aaattaaaat cccattgcag gaaacaccaa
     2521 ctaactgtgg ctttgaaggg ctggcttact cctctcagga ccataccttc tggtttttta
     2581 aagagaaaaa tccgatagag gtctacaaag taaccgggct attgcgtagc gatgagttgc
     2641 atatcagcaa agataaaacg ttgcagcgtc agtttacttt agacgatgtt tctggcgcgg
     2701 aatttaaccc acagaaaaat acgttgctgg tgttgtcaca tgagtcacgc gccctgcagg
     2761 aagtgaccgt gagaggtgac gtgatcggcg aaatgtcgtt aaccaaaggt aagtatggcc
     2821 tttcacacaa catcaagcag gcggaaggga tagcgatgga tgatagcggc aacatctata
     2881 tcgttggcga accgaatctt ttttatcgct ttacgtcgac aaaatcacga taacagtctg
     2941 gcaggttctc cttttttgtt attaacttgt taactcaaaa taatacaaac caggcagaac
     3001 atgaattcga catcgcatcc cgtagaacgt ttttctttca gcaccgcgtt attcgggatg
     3061 ctggttctga ccttaggtat gggtttaggc cgctttctct atacgccgat gctgccagtc
     3121 atgctggcgg aaggcgagtt ttcatttagc gaactctcat ggatcgccag tggtaactat
     3181 gccgggtatc tggcggggag cctgctgttt tcattcggcg cgtttcattt accctcacgc
     3241 ctgcgcccgt tcctgttagc ttccgccctc gcaaccggat tattaatcct cgcgatggcg
     3301 tggctgccgc cgtttcttct ggttttcatc attcgctttc tggcgggagt cgccagcgcc
     3361 gggatgttga ttttcggctc aacactcatc atgcaacata ctcgccatcc ctttgtcctt
     3421 gcggcgctat tttctggtgt tggcgtcggc atcgctctgg gtaatgaata tgtgctggca
     3481 ggcctgcatt ttgccctctc ttcacaaacg ttgtggcaag gtgccggagc actttctgcc
     3541 attatattgc ttgctctggc gctgctcatc ccgtcgaata aacacgttat cccgccagcg
     3601 ccattggcaa aaatcgcgca acaacccatg agctggtggt tactggcgat tctgtatggt
     3661 ctggcgggtt ttggttatat catcgtcgcc acctacctgc cgctcatggc gaaagacgcg
     3721 ggccagcctg tgttgacggc tcacctctgg acactggttg gcttgtcgat tgtcccaggt
     3781 tgctttggct ggctgtgggc agccaaacgg tggggagcat taccttgcct gaccgcgaat
     3841 ttgctggtgc aggcgatctg cgtgctgtta accctcgcca gcagctctcc tttattactc
     3901 atcatcagca gtattggttt tggcggcacc tttatgggaa cgacctcgct ggtgatgacc
     3961 atcgcccgcc agcttagcgt gccgggaaat cttaaccttt tgggctttgt gacactcatt
     4021 tatggtatcg ggcaaattct tggcccggcg ctgaccagta tgctcggcaa cggaacgtcg
     4081 gcgctcgcca gcgccacact ctgcggcgca gcggcgctat ttatcgcagc attaatctgc
     4141 gggatgcaaa tattcaaatt gcatacgaat tattcttaag taatttaact aatttaactt
     4201 ttcagcacat cacgtaaaat aattaaaata tcctttttct ttggaggtac tcagccaccg
     4261 ccggtattat tcctggttta gcaattccac ggctaattca ttatttctgg tcatattata
     4321 gtcaggatcg cattatatct ccgtgctaga aaaaacaagt ttaattctgt tttttcacgg
     4381 aaggtcacta tggcttcgac aaatgcgcat caaataaaaa ataatgatca aaactcttta
     4441 tgtggtctgg gtgacaaaat acgccgcctg accgccggag tttgtttatt tacacaattt
     4501 tttttccccg tcatggcgac ggcacaaaat gtggtacatg ctaaacctca gacgacagtt
     4561 tcatctgccc ccccctctac tagaaaataa tactgtgcct tacacgcttg gtgcgctgga
     4621 atcagcgcaa agcgttgcta atcgcttcgg tatttcactg gaggagcttc gtcgtcttaa
     4681 tcagttccgt acttttgctc gcggctttga taacgtgcgc cagggtgaag aactggatgt
     4741 tccggcaaca acctcgcaga aaagccatga gcaacaaaat gccgtaccgc ctgcgaatgg
     4801 cgaaaacacg ctggagaatc aaatagccag cacctcgcag cgagttggca ctctgctttc
     4861 acaagatatg aatagtgagc aggccagcgg catggcgcgt ggttgggcgt cttcagaagc
     4921 ctcaggcgcg atgactgatt ggttaaataa ctttggtact gcgagaatct ctctgggtgt
     4981 ggatgaagat tttagcctga aaaattcgca attcgacttc ctgcatccgt ggtatgacac
     5041 acctgattat ctgctcttca gccagcatac ccttcaccga acagacgatc gtacccagat
     5101 caacaccggt ttgggctggc gtcatttcac ctccagctgg atgtcaggca tcaacctttt
     5161 ttttgaccac gacctgagcc gctatcactc ccgcgcaggg cttggcgcag aatactggcg
     5221 tgattatctg aagttgagca gcaacgctta tatcggcctg accggctggc gtagcgcacc
     5281 agaattggat aacgacttcg aagcccgccc ggccaacggc tgggatttac gcgcggaagg
     5341 ctggttacct gcctggccac aactgggggg aaaactggtc tatgaacaat actatggcga
     5401 tgaagtggcg ctgtttgaca agaatgatcg tcaaagtaac ccccatgcta ttaccgcagg
     5461 cctcaactat acccccttcc cgcttctgac tctcagtgcg gaacagcgtc aggggaagca
     5521 aggtgaaaat gacacacgtt ttgccgttga tctgacctgg caacccagca gttcaatgca
     5581 gaaacagctt aatccggacg aagtggccgg acggcgcagt ctggccggta gtcgttatga
     5641 cctgattgat cgcaacaaca acatcgttct ggaataccgc aagaaagagc tgattcgcct
     5701 gagtctgctg gatccggtga aagggaagtc tggagaaata aaaccgctgg tttcctcgct
     5761 acagaccaaa tatgccctta aaggctataa catcgaagcc gctgcgctgg aagctgccgg
     5821 aggtaaagtc agcacgtctg gaaaagatat cacggtcacg ctgccaggtt accgcttcac
     5881 taacacccca gaaaccgata atacatggtc gatagacgtt accgccgagg atgtaaaagg
     5941 taacctgtca cggcatgaac aaagcatggt agttattcag gctccgacat taagccagaa
     6001 agattctctg ttatccgtca atccgctaac cgtggctgca gataaaaaat cgacgaccac
     6061 attgaccgtt actgcgcacg attccgacgg aactccggtg ccggggctgg cgctgcaaac
     6121 ccgcagtgaa ggcgttcagg atatcaccct gtctgactgg acagataacg gtgatggtag
     6181 ttacacacag atactgaccg ccggaacgac atcaggttca gtaacactga cgccgcaaat
     6241 taacggtgag agtgcggtaa aagaatccat cgtcgttaat atcgtccctg ttgtctcatc
     6301 ccgcgaccat tcatcaataa caattgataa cgtatcgtat tatgccggag acgacatcaa
     6361 ggttagggtg gaactgaaag acgatagcaa tcaaccggtt gcatatcaaa aagaggaatt
     6421 ggtaaaagcc gttactgtcg aaaacagcaa acctggcgcc acgattgtct ggcacgaaga
     6481 gcagccgggg gtttatgccg cgaattatcc ggcctataag caagggactg cactaagggc
     6541 acaacttagc cttcacaact ggaatgctcc actgcaatcg catatttata acattgaggc
     6601 aaaccagaat aaggctcgcg ttgccacatt atcagcgaca aataatgacg tttacgccga
     6661 taaaaagaca tttaataccc tcacgatcaa cgtcactgat gagagtgata atcccctgac
     6721 aaatcatcag gtcaccttta agaatgaaaa aggaagcgcg gagtttgtcg aaccgccgca
     6781 gcaaaatacg gatgcatatg gtgttgccac aataaacatg gtaagtcagg ttgcggaaga
     6841 aaatacgatt agcgccacgc tgccaaatgg tttttcacaa cggataattg cgaaattcgt
     6901 tagcgattcg agtacgccaa aattcaaaca actggttgcc gatccagata ccattattgc
     6961 tggcaacagc cagggcagta ctctgaccgc catcatcaca gactttcata acaacccgtt
     7021 aaaagatatg aaagtgaatt ttgtggcacc tggtggctcg caactggaca acacgaccgc
     7081 cacaacagac cagtccggta ttgtgcgggt gcacctgacc agttcaaaag ctggtagcta
     7141 ttccgtcgat gcctcgcttg aggtggataa aaatattcac cagtcggtca cgatcaccgt
     7201 ggtcccaaac agggaacaat cggtaatgac cttgaatgcc gggtcgggca gtgcgatcgc
     7261 taacaataca aatatcgtta ccctgactgc cagtgtgaaa gatgtttatg gacacccgtt
     7321 gccggatgag gatgtgaaat ttaccttgcc agcctccatg accgggaact tcacgctaag
     7381 tagtgaaacc gcccgcaccg atgcaaacgg tgatgccgtg gtcacattgc gaggcacaaa
     7441 agcgggtgag tttacagtta cggcgacgct gaccagaaat aataccgttg cttatcagca
     7501 agtcactttt attggggata caaacagtgc gcagctccag ccgctgactg cctcattaaa
     7561 ttccattgtt gcgggtaaca gtacggggag taccctgacg gcaacgatcc tggacgctta
     7621 ccaaaatccg cttaaagacc agttggtcac tttccagagt aacgatgtca ctctaagcga
     7681 aacagaagtc accaccaata cgctgggtca ggcgacggta acaatgacca gcaatattgc
     7741 cggacaacat aacgtcgtgg tgagccggaa agcgcaagct tccgataata aaacgtttag
     7801 tttatcagtg ctaccggatg aaagttcggc gaaggtaata agtataaccg gagccgaaaa
     7861 aacgataacg gtgggcgaaa acatcacgct acggatactc gtccaggacg cgtttaacaa
     7921 tgtaatcgcg ggtcaacgcg tcagattaag tgcgcagcca acaactaaca ttacgatagg
     7981 cgatacggct tacaccgata ataacggtta tgcgtacgtt aaccttctca gcacccaacc
     8041 tggggtttat caggtgacgg caacgctgga caataacagt agtagtaagg ttgacgtgaa
     8101 tgtggcaaat ggcaaactcg agttaacatc atcgaaacca gaaactacgg tccataatag
     8161 tgagggtatt acgctgaccg caacggcgag aaatgcgcgg ggtgaattga tgccagggca
     8221 aattatcacc tttagcgtaa cgcctgaagg tgcaacgcta agcaatacag gggaagtcct
     8281 tactgaccag tcaggtcagg ccaaagtgac gctgaccagt gacaaagtga atgtctatac
     8341 cgttacggcc ataatgggca aagatgttcc cgttcagagc caggtaacgg ttgcggttaa
     8401 ggcagatgct aaaacggcac atgttgtgag cgtcgtggct tctcctgaca ccatcaccgc
     8461 cgacggcatc gatagcagca ccatcacttc acgagtagaa gatgattacg gattcccggt
     8521 tgaaggtgtc gatattagtc atggcttaga caccaaaggc agcccggtag ttaatattcc
     8581 aactacgcgt accgatcagt ccgggcaagt cacggcgaca ataaccagta cattggcaga
     8641 aaccttaaca gtcaatgtgc aagttcctgg cacagccaac caatccgcaa ccattacatt
     8701 ggttgccggc acggccgatg aaagtaagtc aattttgaaa tccgatgttg acactctgaa
     8761 ggctgactac cagcagagcg caaaacttac gctaacattg caagacaagt acggtaaccc
     8821 gatagtgacg tctgatcatc tggaatttgt ccagtcaggc cccttcgtga actttctcaa
     8881 gttgagcgat attgattaca gccaaagaaa ttatggcgag tacaccgtga ctgtcactgg
     8941 cggaaaagag ggaacagcga cactcattcc catgctgaac ggggttcatc aggcaaactt
     9001 aagcatatcg ctgaatctca tccaatcgat aaaagaaatg tccggtcatg tcactgcaaa
     9061 caaccatacc ttctccacgg ctaaattccc gagcgaaggc tttgcaggag cgtattacac
     9121 actcaacaat gataactttg aagcgggtaa aaccgttgat gattatatgt tttcaagttc
     9181 acagggttgg gtgtctgtcg atgcttcggg taaagtttct ttcgcaaata tcggcgatca
     9241 aacgtcagtc acaataagcg ctgttccccg acaaggaggt acaacctacc agaccttaat
     9301 taagctgaaa ggctggtggg tgaataatgg aaatcatacc aatatctggc tagctgccaa
     9361 tgcgctctgt catgctaaaa atgatggata taatcttcct ggcatcacac atttgacgtc
     9421 tggcgaaaac aaacgcacgc agggatcact gtatggtgaa tgggggaacg ttggagcgtt
     9481 ttccagtaat tcgcaattta caccgggagc ttactggaca agtgaatctg atgattacag
     9541 tcggcactac tatgtgcaga tgctaaccgg tatgaccgga agcgacgctg attccagccc
     9601 ccaactgacc gcctgccgta aatcacttta atttcagcag ttgttattaa aggataccct
     9661 tttacgaggg tatcctttca gactgataac aaacgctaca ttgccggatg tgctgcgctt
     9721 atcaggccta tataacccct gcaatatatt gaatttattg tttttgtacc ccagccggca
     9781 taaacgacta ctcaagaaac tcaacagcaa ccgttgatgt cagccagacc ccgttttccg
     9841 cttgccagaa agggatgcct cgtttcgcca tttcctgtgc tttgacggtt aagacaaccg
     9901 gcgatccgtg tcttgctccc actttgcgtg ccgttgcttc atcagcagag agatgcacgt
     9961 aatgacgatc ccctgcaatc agcccctgtt tttttatctc gtcaaggaaa cggcttgccg
    10021 taccgtggta gagaaactgt ggcggtgttt tttcggcaaa ggaaatagcg acctgcgaag
    10081 tcgaatgccc ctgaaccgcg cggatgcacc taccatcact ggaataacta aaacgctttt
    10141 atcgctggtt gcgaccacgg tatccagcaa tgcacgggtt aatcgcttgc ccgctttttg
    10201 ggcacaaagt atgagcttat cgatattcgc ccatccttca cggtccagta cgataccaat
    10261 cgcctccggt ttgtgtcgga ggacaaaact taaaaacttg ctcgtatccg ccagctcttt
    10321 ttcgttgtac tttgccatcg cgttttcgtc cctggatttt ctgtttttat ttaccaatgt
    10381 cattgtcact gggtttatga attttctgca actcaatcct gcgcagaacc ttttccacac
    10441 agcgtaattt ttcccctatg caaaaaacgc atagccagaa acatacttgc attttcccca
    10501 tacccacatg gctgataagg tttttaagtt ccaggaattt tcctggctca agcaacacac
    10561 aaacacaaaa aaacaatggg gattgttatg acacaacaag gggatgctgt agccggggag
    10621 ctggcgacag aaaaagtggg cattaaaggg tatctggctt tttttctcac cataatcttt
    10681 ttttccggtg ttttttccgg aacagacagc tggtggcgcg tttttgattt ttccgttctg
    10741 aacggatcgt ttgggcaact gcctggtgcg aatggagcca ccacctcatt tcgcggcgcg
    10801 ggtggcgcgg gggctaaaga tggctttctc tttgcgctgg agctggcacc gtcagtcatt
    10861 ctctctctgg ggattatttc aattaccgat ggtcttggtg gattacgcgc cgcgcagcag
    10921 ttaatgacac ctgtgctaaa accgttgttg ggcattccag ggatctgctc gctggcgctg
    10981 atcgctaact tgcaaaacac tgatgccgcc gccggtatga ccaaagagct ggcgcaggaa
    11041 ggtgaaatta ccgaacgcga taaagtgatt ttcgccgcat atcaaaccag cggtagcgcc
    11101 atcatcacca actacttctc ttccggcgtc gcggtgttcg cctttctggg cacgtccgta
    11161 atcgtacctt tagccgtcat cctggtgttt aaatttgtcg gagccaacat tttgcgcgtc
    11221 tggcttaact tcgaagaacg tcgcaaccca acgcaaggag cgcaagcatg acaactcagg
    11281 tccgcaaaaa cgtcatggat atgtttatcg atggcgcgcg ccgtggcttt actatcgcca
    11341 cgaccaacct gctgccaaac gtggtgatgg cgtttgtcat cattcaggcg ctgaaaatta
    11401 ccggcctgct cgactgggtg gggcatatct gtgaacccgt aatggcgctg tggggattac
    11461 ccggtgaagc ggcaaccgtc cttttagccg cattaatgag tatgggcggc gcagtcggtg
    11521 ttgccgccag tctggcaacc gctggcgcat taaccggtca cgatgtcacc gttttactgc
    11581 ccgcgatgta tttaatgggc aacccggtgc aaaatgtcgg tcgctgcctg gggacagccg
    11641 aagtgaatgc caaatattac ccgcatatca ttacggtctg cgtgattaac gcattgctgt
    11701 cgatctgggt tatgcagctt attgtttaac aaggagttac catgattgat tataccgcag
    11761 ccggttttac cctgctgcag ggagcgcatt tgtatgcgcc ggaagatcgg ggaatttgcg
    11821 atgtcctcgt cgctaacggc aaaattatcg ccgttgccag taatatccct tctgacattg
    11881 taccggactg cacggttgtc gatctcagcg ggcagatcct ctgcccaggt tttattgatc
    11941 aacacgtcca tttgattggc ggtggcggcg aagcaggtcc cacgacgcgc acgccggaag
    12001 tggcgctaag tcgcctgacg gaagcgggcg tcacgtcagt ggttggtctg ctgggcaccg
    12061 actctatctc tcgccacccg gaatccctgc tcgccaagac ccgtgcgctc aatgaagaag
    12121 gcatcagcgc ctggatgctg accggcgctt atcatgtccc ttcccgcacc attacgggtt
    12181 ccgtggaaaa agacgtggcg attatcgatc gtgtgattgg cgtgaaatgt gccatctctg
    12241 atcaccgttc tgccgcaccg gacgtttatc acctggctaa tatggcggca gaatcccgcg
    12301 ttggcggttt gctcggcggt aaacctggcg tcaccgtgtt ccacatgggc gacagtaaaa
    12361 aggcattaca gcctatttat gacctgctgg aaaactgcga tgtgccgatc agcaagctgc
    12421 tgccgaccca cgttaaccgc aacgtaccgt tgtttgagca ggcgctggag ttcgcgcgca
    12481 aaggcggcac catcgatatc accagcagca ttgacgaacc ggtcgcccct gccgaaggta
    12541 ttgtccgcgc cgttcaggcg ggtattccgc tggcacgcgt caccctcagc tccgacggca
    12601 acggtagcca gccgttcttc gatgatgaag ggaatttaac ccatatcggc gttgcaggtt
    12661 ttgaaacgtt gctggaaacc gtgcaggtgc tggttaaaga ctatgatttc agtatcagcg
    12721 atgccctgcg cccgctcacc agtagcgtag ccggtttcct taacctgacc gggaaaggcg
    12781 aaattctgcc aggcaatgac gcagacttac tggtcatgac gccagaattg cgcattgagc
    12841 aggtatacgc tcgcggcaaa ctgatggtca aagacggcaa agcctgcgtg aaaggaacgt
    12901 ttgaaacggc ttaaacggtt gtcagccgcc ctaatggacg tagaatgccc ccaagggcgg
    12961 ctgacagaga aaaacgtaat ggatgactgt ggtgcggttt tgcataatat tgaaaccaaa
    13021 tggctttaag attttctgac cctggaaaaa tgccgcaatt tttcccaggc ggcagtcagt
    13081 cgtaacgtct cgcaaccggc attcagccgc cgcatccgcg cgctggaaca ggcgattggc
    13141 gttgaattgt ttaaccgcca ggtgacgccg ctgcaactct cagaacaagg gaaaatcttc
    13201 cattcgcaga tccgccatct gttgcaacag ttagaaagca accttgcaga gctgcgtggc
    13261 ggcagcgatt acgcgcaacg taaaatcaag atagccgctg cacactctct ttccctcggt
    13321 ctgttaccgt ccattatcag ccagatgccg ccgctcttta cctgggcgat tgaagccatt
    13381 gatgtcgatg aagcggtcga taaactgcgt gaaggacaaa gtgactgtat tttttccttt
    13441 cacgacgaag atttgctgga agcgccgttt gaccacattc gcttatttga atctcaattg
    13501 ttccctgtct gcgccagtga cgaacatgga gaagcacttt ttgacctcgt gcagccacac
    13561 tttccgttac tgaattacag ccgcaactcc tacatggggc gattgattaa tcgcaccctg
    13621 acgcgccaca gtgagttaag tttcagcacc ttttttgtct cttcgatgag cgagctttta
    13681 aagcaggttg ccctcgacgg ctgtgggatt gcctggctgc cggagtatgc catccaacaa
    13741 gaaattcgca gcgggcagct cgttgtgctg aatcgggacg aactggtgat cccgattcag
    13801 gcctacgcat accggatgaa tacccgcatg aatccagttg ccgaacgctt ctggcgtgaa
    13861 ctgcgcgagc tggagattgt gcttagctga cattctcaag cgtcgcactg cgccaaccgc
    13921 cattatccca gaccagatca aacgttagcg gttcatcgtc gtggtacagc attatctcaa
    13981 ggctgatcgc cagagtgtgg cagtacgctt ctttcaaatt aacctgcaac gccgcaatcc
    14041 gtggctcatg aaataacagt gcagaacgca gctgacggca ataccagctc tggagatgtt
    14101 tgtcgctcgg gttatgcact tgccactcgg gcatgcctga aacaagtaat gacggtaatg
    14161 tcgaatgagg agaattaaaa agagtgagga gatcgctgca taccgattta cgcagcaatg
    14221 acgttttatt gcctgaaatt tcaggtaaaa cagcctgaag tgattggcgc atcattttgc
    14281 gcactcctga cgtttagcaa catatgcggc ttgccctgaa caccgggcac cactgtattg
    14341 atgctcattt tccatagcca ttcccttttg ttcattacat ccatgtcagt ctgagacagg
    14401 cctgaatcct gtcagcaaag tataaaacct tacttgccta tgaatatcta ctgacttttc
    14461 aggaaaaaag tcatgatttg ataaaaatca tcaaactcaa aaaaaaaccg ctagtatatt
    14521 ctgaatattc cgaatagtta cgtagatgaa aatacttgtc accgctgcta cttaattccg
    14581 gtgggaaatt atttatttcc gcataatcag ccatgtatta tttgaaacaa ttagtttgta
    14641 aataatataa attgataaca tatcaaatac atcaaaaaca ccattcacag gcattattaa
    14701 tatagacctc aatatagcaa catcaaattc caattagtaa taagatatga ttttaaattg
    14761 catgcaaata attttaaatg agcgtttttt gatactttag agtgtttcct accctttaat
    14821 atcactgtgt agagttgata tcactacaac agaaacatgc atgtcatttg tttacttctc
    14881 tttttactac agaaaaattt atctattttt ctaaacatca gtgaaatact cataaaaggt
    14941 aactattatg tctatgccat taagcaatgt ccttcagagt caaattatta cagataatca
    15001 cttccttcat catccaaaag tcgaaagtga gttaacacgt aaatatgaac gtgcacgcct
    15061 ggatacggaa aatatctatc ttctgcctct tgctcgtggc aataaccata attacgatgg
    15121 aaaaagcgtt gtcgaaatta gaaaacttga tattagtaaa gaatcctggc catttaatta
    15181 tgtgacagaa gcatgcagag aatccgatgg tattacaaca acaggacgta tgctctatcg
    15241 aaacttgaaa atcacctcag ctttagacga aatatatggt ggtatatgta aaaaggcgca
    15301 tgctgctaca gagttagcag aaggtttacg gctaaattta tttatgaaga gccctttcga
    15361 tcctgttgag gactatacag ttcatgaaat aacattaggc cctggttgca atgttccagg
    15421 ttacgccgga accacgatag gatatatttc aacccttcca gcttctcagg caaaaagatg
    15481 gaccaatgag cagccaagaa ttgatatcta tattgatcag ataataactg tctcaggggt
    15541 agcaaattct tccggattcg cccttgccgc attacttaat gctaatattg agttgggtaa
    15601 tgacccaata attggtatag aagcctatcc aggtaccgcg gagatccatg caaaaatggg
    15661 atataaagtt atccccggtg atgaagatgc gccactcaaa cgcatgactc tgcaaccgtc
    15721 atctcttcca gaactgtttg aattaaaaaa tggtgaatgg aattatattg gcaaataacc
    15781 actgagaaga atgagtacta acattcaggt tatatagata tttaaacaat accctgcaat
    15841 ttacaaagta atactcattg cgacttctta atttaagaaa gcgcaaggaa cgtttaccct
    15901 tgcgcttatt ataataagtc aggcgtcgac tttatccagc ggccatgaat caaacagata
    15961 gccgtcaaaa taaatatcgt caacgttcga gaattccagc agacgttgct taacgttttc
    16021 cagatgctgc cacattgcca gcttcgccgc tcgggcatct tttttgatta acgcggcgag
    16081 gatctgtttg tgatcgccca accactcttt gcgatacagg ctgtcatcca gatggctgtg
    16141 caactggatc cacattgggt tgttttcccg ccactgccag gactgacgga acagctccac
    16201 cagcatgctg ttatgcgttg cttcggcaat agcgagatgg aactgcatgt caccgctttc
    16261 gctgctgccc ggcgcactgg aagccagttc acgctcttcc agttgcaatg cctgacgcat
    16321 tttgacgata tcttcgcggg tagcctgcag ggcggcaaac tcggcgatgt tactttccag
    16381 taactgacgc gcctgtaaca gctcaaaagg acccgcatca ttgcagacgt tggcatccgg
    16441 actgtctgtg ttgtggctgc ctgagctatc aagaacatag ataccggcac cccggcgcac
    16501 ttccaccagc cctttgatct ccagcatgat cagcgcttca cgtaccaccg tccgcgtgac
    16561 atcaagcatt tctgcaattt cacgctccgg cggtagccgt tcaccaggat tgtacggcgt
    16621 ctttatgatc agatcgcgga tcatcgcccc gacttcctgg taaggtcttt gcgcagaggt
    16681 ggcagatttc atggtgttgt gtccgttagg tgcggttaat caaatttttg ccggatgcgg
    16741 cgtgaacgcc ttatccggcc tacaacgggc aattttacgg cccccgttaa tctcaaacta
    16801 taccattagc actggaagtt ggcacgtaca ggtgggcaaa aaaacgaaaa ctggtcaacc
    16861 aaatatcagt ttgattgacc agtaagtctg tagggtaatc agttagttac agcgcagcca
    16921 cacactcgcg cgcaccgcgt tcgcacagct gctgatatgc cgcagtcacc gcgccaacaa
    16981 agtcggcatt ctgcggcaga tcatcggcaa aaataccgct caggcccagc agcgctttca
    17041 cgcggtctgc gccctgatac tgcgcgttaa tcttctggaa ctcggccagc atcgggtcga
    17101 ccacgtcaat ggcattaccc tgctcatcca cgccctgggt gtaacgcatc cagccagcca
    17161 cgcccagcgc caggtgacgc cagctgccgc cgttttgcag gtgcagacgc accgggtcca
    17221 gcagacgctg cggtaacttc tggctgccgt ccatcgcaat ctgccaggta cggtgacgca
    17281 gagacgggtt gctgaaacgc tcgatcagca gcgtcgcata ggcgttcagg tccgtacctt
    17341 ccggcatcga cagcgttggc gcttgttcct gcatcatcag ggcaaaggcc gctttgcgat
    17401 aatccgggtt agtcatggtg tcggcaatag tttcatagcc gccgaggtaa ccgaggtacg
    17461 ccaggaaaga gtggctgccg ttcagcatac gcagtttcat catttcgaac ggcacaacgt
    17521 ctgcaacgaa ctgtgcgccc actttatccc agtccggacg accattaacg aagttatctt
    17581 caataaccca ctgacggaac ggttcgcagg caatggcgca cgggtcgtaa acacccagct
    17641 ggtcagcaat ttcctgtaag gtttctggcg tcgccgccgg aacgatgcgg tcaaccatgg
    17701 tgcacgggaa ggtgacgttc tcttcaatcc atgccgctag ctgcgggtca cgcgcctgag
    17761 ccagccccag taccgcgacc ttcgccacat gaccgttttc acgcacgtta tcgcaggaca
    17821 tcaccgtaaa tgctttcagc cctttttcac gacgcagacg caaggcttcg acgatgtaac
    17881 caatcgcgga cttcggcgca gtcgggtttt ccagatcgtg cttgatcagc gggttattga
    17941 gatccagttg accgcttgcc gcatcagcgc agtagccttt ttccgtgacc gtcagggaga
    18001 caatcgccgt ttgcggacgc gccatcgcgt tgagaatacc ttcgcagccg tcgatttccg
    18061 ggtgcagcgc ttctttcatc gaaccgataa ttttcagctc ggtgctttcc gcgccttttt
    18121 ccgccacggt gtacagcagt tgctgttttt tcaggttttc gatcagcacg cggtcgttgc
    18181 ctggcatcag gttaacttcg cagatacccc agtcgctgtc ggtgctttcc agcagatgat
    18241 gggtgtacag cgcctggtgc gcacggtgaa acgccccgca accgagatgc acaatgcgtg
    18301 attccagacg agaatgatcc catgacgggc gggcaaccgg cagattgctg tcaacaatag
    18361 tagtcatcgt aaactccaga atccgtcttc agagagtgac ggcaaggaag aaggagccgg
    18421 cacggcgcgc agccatgccg gtgggtatta atggctaaag aaagcgcgct ggatcgccag
    18481 ttcgacaccg cgaacttcgg ccaggccttt cagacgacca attgcggagt aaccagggtt
    18541 ggttttcttc ttcaggtcgt ccagcatctg atgaccgtgg tccggacgca tcgggatcag
    18601 gtcttctttg ccttccgctt tacgacggtg ttcttcttca acaatcgctt tcaccacttc
    18661 gtacatatca acgtcaccgt tcaggtgcgc cgcttcgtgg aaggttttcg ggttatcttc
    18721 acgcatggtg gagcgcagat gggtgaagta aatacgcgga ccgaactgct tgatcatatc
    18781 aaccaaatcg ttgtcagcac gcacgccgta ggaaccggtg cacatggtga aaccgttcgc
    18841 catgctgttt acggtatcaa ccatccactg catatcttca atggtggaaa caatgcgcgg
    18901 caggccgagg atcgggcgcg gcggatcgtc cgggtgaaca gccatacgga cgccaacttc
    18961 ttcggcaacc ggaataatcg ctttcaggaa gacagcaaag ttttcgcgca gtttggcttt
    19021 gtcgatatct ttgtacagct ccaggtgctt acggaactgg tcgagggtgt acccttcttc
    19081 cgcacctggc agaccagcga tgatgttacg ggtcagacgc gctttgtctt catcgctcat
    19141 agtggcgaag cgttcagctg cctgagcaat ttcttcttcg gtgtaatccg cttccgcgcc
    19201 tggacgtttc aggatatgca tttcgaatgc agcgaattcg atctggtcga agcgcagagc
    19261 tttggagccg tctggcagca cgtattcgag gtcagtacgg gtccagtcga gcaccggcat
    19321 gaagttgtag cacacggtgc gaatgccgca ctgcgccagg ttgcgcagag tctgctgata
    19381 gttagcgatc cactgctcat agttgccagt gtgggttttg atatcttcgt gaattggtac
    19441 gctttctacg acagaccaca ccaggcctgc gtcttcaacg atcgccttgc gtttgaggat
    19501 ctcttctacg gaccatactt cgccgttcgg gatatggtgc agcgcggtaa ccacgccagt
    19561 tgcgcccgcc tgacggacat cagctaaaga aaccggatcg tttgggccgt accagcgcca
    19621 ggtctgttcc atgatttttc ctctttcaca tagtgggttc cacagcaaag agacgcggaa
    19681 ccctggagtt gtttaggtat ccggatgaac aaccagaccg cagccgtggc gttgcatcgg
    19741 atgagatggc gtataagttc taccttttgg taagacatgt caacccgaat tctgaaattg
    19801 gttaaccaca tcacaagaat ttcactctca tggttgttgc tgcaaattgc agcaaagggt
    19861 aatcgatggt tattgatcag aggatgtgac attcatcgca acaatggttg accaatttac
    19921 ataacatgtc gcccaaaata acaacggttc aaccaggtaa ggtaaaacgt tatgcatgtg
    19981 cttaacattc tctgggtggt attcggcatt ggtctgatgc tggtactgaa tttgaagttc
    20041 aaaatcaatt caatggtggc tttgttggtg gcggcgctgt ccgtcgggat gctggcgggc
    20101 atggatttga tgtcgctgct gcacaccatg aaagcgggct tcggcaacac gctgggggaa
    20161 ctggctatca tcgtggtgtt cggtgcggtc atcggtaaat tgatggtcga ctccggcgcg
    20221 gctcaccaga tagcgcatac gctgctggcg cgtctcggtc tgcgctatgt acagctgtcg
    20281 gtgattatca tcggcctgat ttttggtctg gcgatgttct atgaagtggc ctttatcatg
    20341 ttagcgccgc tggttattgt cattgccgcc gaagctaaaa ttccgttcct gaaactggcg
    20401 atcccggcag tagcagctgc cactaccgca cattcactgt tcccaccgca gccgggtccg
    20461 gtggcgctgg tgaatgctta tggcgcagat atggggatgg tttatatcta tggcgtactg
    20521 gtgacgatcc caagtgtaat ctgcgcaggt ctgatcctgc cgaagttcct cggcaatctt
    20581 gagcgcccaa cgccatcatt cctgaaagca gatcaaccgg tagatatgaa caatctgccc
    20641 tctttcggcg tttcgattct ggtgccgctg atcccggcga tcattatgat ctctaccacc
    20701 atcgccaata tctggctggt aaaagatacc cctgcctggg aagtggttaa ctttatcggt
    20761 tcctcgccga ttgcgatgtt tattgcgatg gtggttgcat tcgtactctt tggcaccgcg
    20821 cgtggtcatg acatgcagtg ggtgatgaac gcttttgaaa gcgcggtgaa gagtattgca
    20881 atggtgattc tgatcatcgg tgcgggtggc gtgctgaagc aaactatcat cgacaccggc
    20941 attggcgaca ccatcggcat gttgatgtcc cacggcaata tctcgcccta cattatggca
    21001 tggctgatca ctgtgctaat tcgtctggcg acgggtcagg gtgtcgtttc ggcgatgacc
    21061 gccgccggga ttatcagtgc tgcaatcctt gatccagcaa ctggtcagct ggttggcgtg
    21121 aatccggcgc tgctggtgct ggcgacggct gcgggttcca acaccctcac ccacattaac
    21181 gatgcatctt tctggttgtt caaaggttac tttgacctgt cggtaaaaga cacgttgaaa
    21241 acctggggtc tgctggagct ggtcaactcc gtggttgggc tgattattgt gctgattatt
    21301 agcatggtag cgtaaaagaa caccagagcc tgccaatggc aggctcagtt cggattgtcg
    21361 gcaaagtgcg ctttgtttat gccagatgcg acgctgacgc gtcttatctg gcctacaaag
    21421 ggctaacgtg caggttttta gcttcaggta atattgcgta cctgcattag caatgccctg
    21481 tgatttcttt attgataaac aaaagtcacg ccaataatcg attgcacatt ccctgcagtc
    21541 acctgcccgc cggtacgtgc gtaatttgcc gttaatccca gacttaccgc cgaagttcct
    21601 actgctccta acgataccgt gttattcgct ggaataatcg taccgttgcg cgtcaactgt
    21661 acgccgacgc cctgcgctgg tgaaaacgac gcggtattgg tgaaaatcga gttgcccgca
    21721 tctgcggttg tgccggagag gtaatacccc aggttttggc ttttcgcaca ataaacggta
    21781 agaggaattg gcactgaacc agggtagtcc ggcagagtaa cggtgacatc acgagcagaa
    21841 acatcgcagc cgccagtagg cactaccaca tcattattgg cgtaaatatt ccacacaaac
    21901 tggaaatcat cgctgttata gttgttggtc tgtcgcaaaa taagcacggc aattaatgag
    21961 ccagctttaa tcgccacccc gcccgcactg ctcacaggcg tcaaataaag cgccaccggc
    22021 cacggcttat ccgttctcga attataaaca acccgcggcg tttcgctggt ggtcggaaat
    22081 ggatagctac tgccactata ttttacggtc ccggaaaaat tagataacac gccgccataa
    22141 gccgagcctc gttgcagtgt gacatagtct gtaatggttt ccggataatc gttatggcaa
    22201 aagatttgcg tcgaaagatc tacgaccagg ttttgcccca cattcacggc aggcgcaagg
    22261 tttacataaa cattagcgct gccaccgcca atagggatag cggtaccatt ggcggtttta
    22321 caggcgaatg accaggcatt taccgaccag cccatcagca gtacagcaaa cagggtaata
    22381 actcgtttca ttacaattat ctcttcgggt tcagctgtag gtataggtga tgctaatcac
    22441 tgcctgaatg gttccctgag tggctccgcc atttaccgtc aatgctctga cctgtaacgg
    22501 gaagtgcgct gattgtgagg aatcatccac ctgaactgtt ttggttgcgc cagtattcaa
    22561 tgtgttgcca ctgtcatcct gtagctctaa ctggatgttt tgcgcggtcc cctggttttt
    22621 ataatatccg gtactgtcgg ctgccccgct gaagctggca gtgacccttg acgttcccac
    22681 cggacaatta gtcaactcaa gcgcaacatc atgccaggcc gatgccgccc cggcagacat
    22741 cagactgaaa gaataaagat cgccgagatc aaccgtggca ttggtggtgg aaactgtgca
    22801 cggtttggcg acgaccttac cgttcaccgt gatggtgaca tcggctgcct gtatcgtcgc
    22861 acttgcgagc gccaatattg ccgccaataa atacccacgt ttgcgccatt tcatgagcat
    22921 ctccagttac tgatattcaa gagtgaaggt agccgtggca ttgatatgcc ccgcagtgac
    22981 aggcacctgt gtcgccatta gccgggcgta aaaattcaac gtatttggtt tacccggcgt
    23041 caggggcgtc cacgaaatcg cggacgatgg agcattaagg ggtatctgat tttgctgctc
    23101 attcagaagc tgtattccca gtcccgaagc cgctgacacc gtattttcaa gtgcaagcag
    23161 gttggcattg tggctatctg caacgccggt aaacccaact tttacggcag aaacggcgtt
    23221 accacagggt gacagcaaaa tacgaaatgg aacgacagga gtcgtcgcgc caatgttgtt
    23281 aaattgcttc gccgcgtttt ccatcagatc aacagtaaaa ttggttgatt cagcggccac
    23341 actacagccg ttatctctga catagccgcg gatagtaatc gtgctatccg cagccaaagc
    23401 gtgactcact gccagccaca aaaaagcgca cagaagataa aaaggtttgt ttctcatcac
    23461 gcccccttaa cgacattcag ctgatagctg ggttaataac tgctgctgac tctctggtgg
    23521 cagttgataa ttggcgacac aatgagcatt ttcctcttct ccccatttca cctgaacttt
    23581 tcccgctaga ggcattccgc tgaggtaaac ctgaccatta tccgcaacaa tgccgctact
    23641 ctggctactc tctgatgtca ccatcgcccc aaacggcagc ggcttattat tgtgggttag
    23701 cgtcatgagc agttttatcc caacgcgcgc tttaaactct gctcgcacga tcgccccacg
    23761 agtgggaaca acgttagcga ccgcgttatt taaatcgacg ttatcagcca gggtattggt
    23821 atccagcgcc actctatttt cccgatattc agtggcataa ggcagcacgg cataaccgcg
    23881 ccagtcggta cgcacccccg tctggttttc gacttttgca tcttttgcgc caggcgcttt
    23941 aacaagcacc accgtatcgt ttaacggctg ccccagcgtt acgccattgg catgagccag
    24001 taccccaccg ctgactccgt aatagagctg cttaatatca tcgctatggc tgtaaccgat
    24061 attggcattg ccgtaaccac cgcgataatt cagcgtggcg tagcctgtgc ttccgctatt
    24121 accatcgcct cccccggcat agccggtttg cacgctatag ctgaggttgt tgtcttccag
    24181 caacgtaccg tatacaccag ccagattggt catccgaccg ttgagatcgt gtgacatgct
    24241 gtagctggca ctggcatgtc gccactgaga tttactgtca gaacgcagcc agtggctgaa
    24301 aggaatattg acgttacgcg ctaacatctg atcacgtcct ttttgccagg cgtttttcgt
    24361 caggctatag ctgagcgtcc agttgatatc ttcgaacgca gtatttaatc cagcctggaa
    24421 ttgctcatcg acattactcg ttccccaata agtttgatgg ctaccactca aatacagtgt
    24481 tgatgagcgc ccgagttgct gagtaacggt gagttgtaat ttcccgcgtt tgttataagc
    24541 gaggttgtaa tagtcggtga atttcggctt aacctgaata actccgtcct gtgtttcgat
    24601 gttgtagcca ttcattcgac tgtatgttgt atcagcgaaa ttaaaatatc cgctggtcga
    24661 ataacggtaa cccactaact gaatattcgt gcctgactca ttgagcgatt tgttatagag
    24721 aaaacgcacc gattgtccgt catgctgact gtcatcggga agtgtggaat tagcctgagt
    24781 catatccaca gacagagcgc ccagtgcccc catatttttc ccgataccaa aattaaaagc
    24841 acgataacga tctgccagtt gcgttccacc atatattgtc cagccagctg gaaggccgtg
    24901 gagtaatgta ctttggaaaa agcggggttt ttcctgttgc gcatttccac tacggtattc
    24961 tcctgccgta atggaataac gagtatgccc ttcacgttgc aaaagcggga ctgacgaata
    25021 gggtacggta aaaatctgcg tgctgccgtc agcctcttta atcgttacct gcaagtcacc
    25081 actattacct gcggcataga tatcgttgat ggtaaaaggc cccggcggca ccgtactatt
    25141 ataaatgtca tacccatttt gtttaatagt gacctgtgca gtaccacgag caataccgtg
    25201 gatcaccggg gcaaatcctc tttggctatc gggtaacata ttgtcatctg aggccaattg
    25261 tgcgccgcga aagttaatac catcgaaaat atcaccctga gtataaccat cacccagcgt
    25321 cagccgggaa cgtaacggta ttatgtctcg ctcaagccag gtattgatat gctgccattt
    25381 atttttgcta cctgatgatc tgtcgctact gttataactc caggtggtat tgtcgcgtaa
    25441 acgccacgca ccaatattta acccactctg taggtttaaa tatgcataat ggctgttacc
    25501 cccaatccga ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat
    25561 accgggatcc cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg
    25621 agggatcgtc aggttcagtc gctgctgacc aacatctaaa tgcgcagtag cgtcctggac
    25681 cattgtggtt aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc
    25741 cgtattcagc cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc
    25801 actgtcgccc gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat
    25861 atcgacgcga tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc
    25921 cacagcctgg ggatcatccg ctaaaaagcg cggattaaaa tagagttcgg cagatgacaa
    25981 aggtgcctgt acggcaaaag cacaggcgac aaagagccgg acaaaaaaac cagccaaacg
    26041 atgcttacga atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga
    26101 cattattcct ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt
    26161 ccccctgcgt tattccatta cgcccgtcat tttgggggta agtgcaccat aatcatttat
    26221 tgttcggtaa gtaatattgc ttcccgcatc agaaggcaat ttaaccgtgc tttcgcccat
    26281 tggaggcacc aaggcatttt caagaacccg ggctccggca ttcaactctg ttaccgtcag
    26341 gtaatagggt gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt
    26401 ttctgcggcc tgatcgggtg gcaacgctaa tttagccgga cgatagtaca gtttaatgcg
    26461 gctgataatt gcgagctgta gcgtattctc agtcaatttt gatttatcca ttgacggaat
    26521 cgctttaacg ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc
    26581 atcaagaata cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac
    26641 gataaaacga ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt
    26701 actattttca tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat
    26761 tatgcgagtc gcacctaagg ccactcccgc ttcagcgcgt ccggcaacca tcattgccat
    26821 gaacatcagg atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac
    26881 gtttttatta ctcactgtcc tgttcctgtt atcacattat ctgctgaaca attactgata
    26941 ggttaaagag aaccaggcct gtgcattggc gatgccgcca gtaacccgac gcccggtagc
    27001 acgatatttg gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc
    27061 tggaggacga ttaatcggta cgaggtttcc ttcatcatca aacaacgcta cgccaatatt
    27121 ggtggctatc cctggcccct ctcccacgga aagcacatcc ggatttttac catccgcgac
    27181 accgtgaaac gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat
    27241 aacaaaaggc accggtgcgc tatcttcccc taccgcatga aaccggttac tgctgatttg
    27301 ccccatattg accgtcattt gtttatcacc ggcttcaatc aggcaagttt ccgcaataat
    27361 gacgccctga aattgcatat ttccgccggg cggcgtggta ttccatttat ttccggccag
    27421 agcaaacatt ggcagcaacg acgctaatag aaataatctc ttccttttca tcgttatttt
    27481 tatcgcacaa gggtgggcat ccctgcccgt aatgacgtcc ctgaacctgg gtaggttatt
    27541 gatactgaac cttgaaggtc gcatccgcat tagcagcacc cggggttgcc tcgccgattg
    27601 cataataacg cgcctggaac ggaatggtgt tagtaccgtt attcagggtt gtttgctcac
    27661 tgaatgtcgc accatccagc gtcagcgcag cacccgttct gtccaggatc tgcacaccaa
    27721 cgtttgttgc gctacccgca gctgaactct gcagagccag aacgttggta tgacccgcat
    27781 caatcgccgt acctaaaaag gcaacagcgg ctttagatgc aacattggta tcgcaatcat
    27841 tcagctgaat gttaaaaccg acagcagaac tggttgctcc gtcctgtgcc agcgatgcgg
    27901 tacgaacctg tcctaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg
    27961 cgttaacaac ttccccttta aagtgaacgg tcccaccatt aaccgtcgtg gcagcggcca
    28021 gagccgctgt agaactgagg gacagagccg acagaacaac gattgccaga gttttaattt
    28081 tcatgctgct ttcctttcaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca
    28141 ctttgttttg tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa
    28201 catttggggc cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt
    28261 tgctggatta atattctatt gttatcttta tttatagatt tttatattgc atgaggtggt
    28321 ttttggagag aagaatgagg aagatgcgtc gagccacaga aacgttagct ttacatatag
    28381 cggaggtgat gtgaaattaa tttacaagag aaataattta catatcaaac agttagttta
    28441 aaatttttat gcttgagaaa aaatacgtaa c
//
LOCUS       DAZFBR010000059        28326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFBR010000059 DAZFBR010000000
VERSION     DAZFBR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.059"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            358..702
                     /locus_tag="ACHJZ2_002742"
     CDS             358..702
                     /locus_tag="ACHJZ2_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568821.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHGFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            780..971
                     /locus_tag="ACHJZ2_002743"
     CDS             780..971
                     /locus_tag="ACHJZ2_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4568822.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(952..2130)
                     /locus_tag="ACHJZ2_002744"
     CDS             complement(952..2130)
                     /locus_tag="ACHJZ2_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4568823.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     DQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            2312..3736
                     /gene="sbcB"
                     /locus_tag="ACHJZ2_002745"
     CDS             2312..3736
                     /gene="sbcB"
                     /locus_tag="ACHJZ2_002745"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO4568824.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(3779..4006)
                     /gene="tsuB"
                     /locus_tag="ACHJZ2_002746"
     CDS             complement(3779..4006)
                     /gene="tsuB"
                     /locus_tag="ACHJZ2_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO4568825.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(4020..5078)
                     /gene="tsuA"
                     /locus_tag="ACHJZ2_002747"
     CDS             complement(4020..5078)
                     /gene="tsuA"
                     /locus_tag="ACHJZ2_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO4568826.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(5257..6615)
                     /gene="plaP"
                     /locus_tag="ACHJZ2_002748"
     CDS             complement(5257..6615)
                     /gene="plaP"
                     /locus_tag="ACHJZ2_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO4568827.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(6605..6667)
                     /gene="yoeI"
                     /locus_tag="ACHJZ2_002749"
     CDS             complement(6605..6667)
                     /gene="yoeI"
                     /locus_tag="ACHJZ2_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO4568828.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(6882..7811)
                     /gene="yeeY"
                     /locus_tag="ACHJZ2_002750"
     CDS             complement(6882..7811)
                     /gene="yeeY"
                     /locus_tag="ACHJZ2_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4568829.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHNVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(7857..8681)
                     /gene="yeeZ"
                     /locus_tag="ACHJZ2_002751"
     CDS             complement(7857..8681)
                     /gene="yeeZ"
                     /locus_tag="ACHJZ2_002751"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4568830.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVKAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            9107..9157
                     /gene="hisL"
                     /locus_tag="ACHJZ2_002752"
     CDS             9107..9157
                     /gene="hisL"
                     /locus_tag="ACHJZ2_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO4568831.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    9133..9256
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            9303..10202
                     /gene="hisG"
                     /locus_tag="ACHJZ2_002753"
     CDS             9303..10202
                     /gene="hisG"
                     /locus_tag="ACHJZ2_002753"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO4568832.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            10208..11512
                     /gene="hisD"
                     /locus_tag="ACHJZ2_002754"
     CDS             10208..11512
                     /gene="hisD"
                     /locus_tag="ACHJZ2_002754"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO4568833.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            11509..12579
                     /gene="hisC"
                     /locus_tag="ACHJZ2_002755"
     CDS             11509..12579
                     /gene="hisC"
                     /locus_tag="ACHJZ2_002755"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO4568834.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            12579..13646
                     /gene="hisB"
                     /locus_tag="ACHJZ2_002756"
     CDS             12579..13646
                     /gene="hisB"
                     /locus_tag="ACHJZ2_002756"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO4568835.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            13646..14236
                     /gene="hisH"
                     /locus_tag="ACHJZ2_002757"
     CDS             13646..14236
                     /gene="hisH"
                     /locus_tag="ACHJZ2_002757"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO4568836.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            14236..14973
                     /gene="hisA"
                     /locus_tag="ACHJZ2_002758"
     CDS             14236..14973
                     /gene="hisA"
                     /locus_tag="ACHJZ2_002758"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO4568837.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            14955..15731
                     /gene="hisF"
                     /locus_tag="ACHJZ2_002759"
     CDS             14955..15731
                     /gene="hisF"
                     /locus_tag="ACHJZ2_002759"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO4568838.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            15725..16336
                     /gene="hisIE"
                     /locus_tag="ACHJZ2_002760"
     CDS             15725..16336
                     /gene="hisIE"
                     /locus_tag="ACHJZ2_002760"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO4568839.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(16433..17410)
                     /gene="wzzB"
                     /locus_tag="ACHJZ2_002761"
     CDS             complement(16433..17410)
                     /gene="wzzB"
                     /locus_tag="ACHJZ2_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO4568840.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(17556..18722)
                     /gene="ugd"
                     /locus_tag="ACHJZ2_002762"
     CDS             complement(17556..18722)
                     /gene="ugd"
                     /locus_tag="ACHJZ2_002762"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO4568841.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(18971..20377)
                     /gene="gndA"
                     /locus_tag="ACHJZ2_002763"
     CDS             complement(18971..20377)
                     /gene="gndA"
                     /locus_tag="ACHJZ2_002763"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO4568842.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(20578..21243)
                     /gene="perB"
                     /locus_tag="ACHJZ2_002764"
     CDS             complement(20578..21243)
                     /gene="perB"
                     /locus_tag="ACHJZ2_002764"
                     /EC_number="2.3.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine N-acetyltransferase"
                     /protein_id="HFO4568843.1"
                     /translation="MNLYGIFGAGSYGRETIPILNQQIKQECGSDYALVFVDDVLAGK
                     KVNGFEVLSTNCFLKAPYLKKYFNVAIANDKIRQRVSESILLHGVEPITIKHPNSVVY
                     DHTMIGSGAIISPFVTISTNTHIGRFFHANIYSYVAHDCQIGDYVTFAPGAKCNGYVV
                     IEDNAYIGSGAVIKQGVPNRPLIIGAGAIIGMGAVVTKSVPAGITVCGNPAREMKRSP
                     TSI"
     gene            complement(21287..22218)
                     /locus_tag="ACHJZ2_002765"
                     /pseudo
     CDS             complement(21287..22218)
                     /locus_tag="ACHJZ2_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(22506..23876)
                     /gene="cpsG"
                     /locus_tag="ACHJZ2_002766"
                     /gene_synonym="manB"
     CDS             complement(22506..23876)
                     /gene="cpsG"
                     /locus_tag="ACHJZ2_002766"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO4568844.1"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKAL
                     LKLLSE"
     gene            complement(23880..25328)
                     /locus_tag="ACHJZ2_002767"
     CDS             complement(23880..25328)
                     /locus_tag="ACHJZ2_002767"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFO4568845.1"
                     /translation="MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTT
                     LLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHA
                     LKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGY
                     IERGELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLE
                     ELRKFRPDIYNVCEQVASSSYIDLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPV
                     DIGWSDVGSWQSLWDISLKSKTGDVCKGDILTYDTKNNYIYSESALVAAIGIEDMVIV
                     QTKDAVLVSKKSDVQHVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVKK
                     IIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLENP
                     GIIPLNLIEVSSGDYLGEDDIIRQKERYKHED"
     gene            complement(25310..25819)
                     /locus_tag="ACHJZ2_002768"
     CDS             complement(25310..25819)
                     /locus_tag="ACHJZ2_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO4568846.1"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHY
                     IVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
                     KDIICLMRQ"
     gene            complement(25822..26787)
                     /locus_tag="ACHJZ2_002769"
     CDS             complement(25822..26787)
                     /locus_tag="ACHJZ2_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFO4568847.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKL
                     LFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
                     VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMA
                     SASIYVMEMPYDIWQKNTKVMLSHINIGTGIDCTICELAETIAKVVGYKGHITFDTTK
                     PDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYRG"
     gene            complement(26790..27908)
                     /gene="gmd"
                     /locus_tag="ACHJZ2_002770"
     CDS             complement(26790..27908)
                     /gene="gmd"
                     /locus_tag="ACHJZ2_002770"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO4568848.1"
                     /translation="MTKVALITGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERI
                     DHIYQDPHGSNPNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVA
                     KLYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSF
                     VGKGIEEKGIVDSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWR
                     PEITLAEMISEMVAKDLEAAKKHSLLKSHGFSVSLALE"
     gene            complement(27928..>28326)
                     /locus_tag="ACHJZ2_002771"
     CDS             complement(27928..>28326)
                     /locus_tag="ACHJZ2_002771"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4568849.1"
                     /translation="MVLAKKLGIESKIKILGHIPKLEQIELIKNCIAVIQPTLFEGGP
                     GGGVTFDAIALGKKVILSDIDVNKEVNCGDVYFFQAKNHYSLNDAMVKADESKIFYEP
                     TTLIELGLKRRNACADFLLDVVKQEIESRS"
BASE COUNT         7574 a         6952 c         6500 g         7300 t
ORIGIN      
        1 agaaatacga tttcgatatc tgggtattct ctccatccag aggtgttctt gacgggctac
       61 agtgggatgg ctcgatgttt actgatgatg aatatcagtt cgttattcat gacgccacgc
      121 attggatgag aaaagtatat ccagcagcac cgcagcagga tggtgaatga tgccgccagt
      181 taaagttgtg attatcactt tggtgatgat agtgattgcg agaatcatgt ctggtgaaat
      241 tgtgtggata tggtaatggc taaggcagca gcagagcgca actaacaatc ctcgcactcg
      301 cggggatttc ttttatctga actcgctacg gcgagttttg ttttatggag acaagaaatg
      361 tcagatttgg ctatgaaggt tttgaaatgg caatcaactg gcgatgtcgg catcagtagc
      421 gcaactcttg cctcaatcgc atgtggcctg aaaaagaata tctatggtca tggcttcggt
      481 gctccacatg acgcagccga tttcagacga tgcgttgcac ttgttgagca gattccagaa
      541 atcagagatt cattcgacaa ggttgcaaag cgcgttccgg cattcaaagg aatcctcaac
      601 gaatgggatt ccctcgttgc tctgttgaag tctgaaatga agatacacgg aaacaaagca
      661 ccagagactt acagaagaat cagcgagcta cgcaaggact aacgcctcac actcgatgag
      721 gcctgttcat atctgataga gccgctatat ggcggtttct ttttgcctgg agaattaaga
      781 tgaccgatac cagcctgatt cctgagaaag aagtgatgaa caagctcggt gtttcatcac
      841 gtcagacaat ctggaactat accaaacggc acggatttcc gaagccagtc agaacccacc
      901 ccaaatcata ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga
      961 aatgctcctg atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc
     1021 ctctatccag tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat
     1081 tcgctcagta acatgaggct cgacattcat ctcggacaga cgtgtaacaa gcgttcttct
     1141 gaaatcatgt gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg
     1201 gttagctgca gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt
     1261 gtacgttgtc atggcctttt cgagaaaagg cttaatttga tcgaagattg gacggcggat
     1321 aacattaccc atcttgctat gctctttcgg tacggtccat acgttgtcca gcaggtcaaa
     1381 ttctgtcttt gttgccagcc taagctctga gagcctcgcc ccccacagca taagcatctg
     1441 atgaaggagc ttatttgacg tagacgcacg gcttctttca atagcaagcc agatcttagc
     1501 cagttcgtga tacgacagta cccgatcccc tacctcagcg cgggagccga agtcccttgg
     1561 ttggatgctc ataattgcgc aactatctat caactgacgc cgcatgcacc aactaattgc
     1621 tgatcttagt tgacttagca cctgccttgc tcggcgtgga ttatcttttt cttcttcggt
     1681 aagcaggtca acccattgct taaccgtgat agaagatgcc ggacgattag ggaaggcgtc
     1741 atgcatgcgc ttcataaccg ttgatcggta aagtgcttgg gtcttttctc tgagagttgt
     1801 agagacgtag ttgtcgaacc agtagtcgag acactgggcg accgtcatgg agttctccac
     1861 cttctcttca aaataagtgc gtggatccgt tcctgagaaa tagagctttc gcaagtcagc
     1921 agtgatctgt ctggcatcct tcaaagacag ggatgggtat cgaccaagcc caagtcgatt
     1981 aggcttgcca tgccagcgat agcggtactg gaactggatg acccccttcg gtgaaattcg
     2041 tacgctgagg ccatcggcat cagccacttc ttgtgggcca gaatatggtt taccataaat
     2101 agtacgcagt tttgtgtcgc ttatagccat aaataatatt tggtacgctc agaaataatg
     2161 gtttggtaca catctggtac acaatatcac atggacaaca gcgcacagta aacaactata
     2221 tttgacgttc gtagacatac tcaggtgaaa aaagggttgt ttttcgagtt ataacagaca
     2281 gttaattaac tacattggac aatgctagac aatgacagac acagataagc aacctacctt
     2341 cctctttcac gattacgaaa cctttggcac gcaccccgcg ttagatcgcc ctgcacagtt
     2401 cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc gaacccgaag tcttttactg
     2461 caagcccgcg gatgactatt taccccagcc tggagcagta ttaattaccg gtattacccc
     2521 gcaggaagct cgggcgaaag gagaaaacga agccgcattt gccgcccgta ttcactcgct
     2581 ttttaccgta ccgaagacct gtattctggg ctacaacaat gtgcgtttcg acgacgaagt
     2641 cacacgcaac gttttttatc gtaatttcta cgatccttac gcctggagct ggcagcatga
     2701 taactcgcgc tgggatttac tggatgttat gcgtgcctgc tatgccctgc gcccggaagg
     2761 aataaactgg cctgaaaatg atgacggtct accgagcttt cgccttgagc atttaaccaa
     2821 agcgaatggt attgaacata gcaacgccca cgatgcgatg gctgatgtgt acgccactat
     2881 tgcgatggcg aaactggtaa aaacgcgtca accacgcctg tttgattatc tctttaccca
     2941 tcgtaataaa cacaaactga tggcgttgat tgatgttccg cagatgaaac ccctggttca
     3001 cgtttccgga atgtttgggg catggcgcgg caataccagc tgggtggcac cgctggcgtg
     3061 gcatcctgaa aatcgcaatg ccgtaattat ggtggatttg gcaggagata tttcgccatt
     3121 actggaactg gatagcgaca cattgcgcga gcgtttatat accgcaaaaa ccgatcttgg
     3181 cgataacgcc gccgttccgg ttaaactggt acatatcaat aaatgtccgg tgctggccca
     3241 ggcgaatacg ctacgcccgg aagatgccga ccgactggga attaatcgtc agcattgcct
     3301 cgataacctg aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg tggcgatatt
     3361 cgcggaagcc gaaccgttta cgccttcaga taacgtggat gcacagcttt ataatggctt
     3421 tttcagtgac gccgatcgtg cagcaatgaa aattgtgctt gaaaccgagc cgcgtaattt
     3481 accggcactg gatatcactt ttgttgataa acggattgaa aagctgttgt tcaattatcg
     3541 ggcacgcaac ttcccgggga cgctggatta tgccgagcag caacgctggc tggagcaccg
     3601 ccgccaggtc ttcacgccag agtttttaca aggctatgca gaagagattc agatgctggc
     3661 gcagcagtat gccgacgata aagaaaaagt ggcgctgtta aaagcgcttt ggcagtacgc
     3721 ggaagagatt gtctaatgtg aagcctccgc cgctggtacg gcggaggaca atgttgagtc
     3781 aggctttttg aacggtgatg ctccacgctg catcgccaat ctgctgataa tcggtgatgg
     3841 catgcccttc ttctgccgcc cactgtggga ttgcttcagt tgcctgggtg caatcaaact
     3901 caattaccaa ctcatcgccg ctggccattt ctgccagtgc tgcttttgct tctatgagtg
     3961 gaaacggaca gacctgcgtc accacatcca gctttttaat cgccatcttt acaccttact
     4021 taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg gtcgaacata cacaagccag
     4081 gacgcagtcc agactccgag aatcataaat acaaggccaa cccagccctg ccaggtcatc
     4141 attgcagtca taaccagtcc attaccgata gagcaaccac ctgcaatact ggcaccgaag
     4201 cccatcagta caccacctag cccgctacgt actgttgttt gtgcatcagc tgcgcgaacg
     4261 cggaactcac ggctcgcttt ggctgcaata aatgccccca cgaagatccc taacaccagg
     4321 aaaacgcccc agttaatgta tttcacgtca cccgcgacca gaaattgcag aatattggcc
     4381 gttggggaag tgatgcctaa cccaaacata cgtccggttg cttcactcag gggccaggct
     4441 aaaagcgcaa tcaaaccgat gagtacagct gtgacaaagg gatgccagcg tttttcaaac
     4501 agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag ttttggcttc
     4561 ttcaactctt tcatcaccac ccagagcgtt atcaccagca aaacggcaac caacggccac
     4621 acagacaaat taaaagtctc agcaatagag ttatgttcag tagtgtagtg ctgcaaggtt
     4681 tgatttaaac cactggcgtg tggagaacgc atcaccgcac tcatcaccat ataagtgaaa
     4741 agcgcgatcc aactgccgat caatccctca cccgcgcgat accaggtccc ggtggcacat
     4801 ccacccgcca gaacaattcc cagcccgaag agatacccac ctataacagt accaagccac
     4861 gggaacgccc cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa aacccccacg
     4921 ctttgtaccg aaattgcaat cagcaaggcg taaaacatgc gattattttt cgcgatatac
     4981 atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct gcatgacaaa tcccagcaga
     5041 gcaccacaaa ttagcccgct taatatcatt gaaaacataa attaattaac cagatgaatg
     5101 ttaatgggga aattattcat gactggtgga atgcagacca ataaccaaat tctctaaatt
     5161 agaacaaatg gttattaatg aggatttcga taaaaaagaa taaaaaaacc ggagccaaat
     5221 gttcattcga ctccggttta ttattagaaa tgatgattat gctacgtctt cgtactgcgg
     5281 aaccgggtta cggaagctct tggtgacgca agccaggtaa atcagaccga tagcagccca
     5341 gatcagaccc agaaccattg agctttcttc caggttaacc cacagcgcac caacggtcag
     5401 cgcaccacac atcggcagga acagatactg gaagtgatct ttcagcgtct tattacgctt
     5461 ctcacggatc cagaactgcg agatgaccga caggttaacg aaggtgaacg ccaccaacgc
     5521 accaaagttg atcagcgccg tagccattac caggtcgaag ttgattgcca gcagggcaat
     5581 cgcgccaacc aggatgatgt tcatcgccgg agtacgccat ttcgggtgta cataaccgaa
     5641 gaaacttttc gggaacacgc cgtcacgacc cattacgtac atcagacgcg ctacgcctgc
     5701 gtgcgccgcc ataccggacg ccagtacggt aatggtggag aagatcagcg caccgacctg
     5761 gaacgcttta cccgcaacgt acagcatgat ttcaggctgt gacgcatccg gatctttaaa
     5821 gcgagagata tccgggaagt acagctgcag gaagtaagtt gcaaagatga agatcatgcc
     5881 gccgatcagc gcggtcagga aaatcgcacg cgggatcacg cgctctgcat ctttggtttc
     5941 ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga ttgtcgcccc
     6001 ggtaatcatc gggataacat gcgcatcgcc agaccagaat ggacgagtgc tcgccagcgt
     6061 accagcgcct tcaccttcaa atacgccata aacaaccatg cccagaatca ccgcgatcag
     6121 cactacctgc aacacgacga ttacagtgtt gaagttcgct acggatttca gactacgcag
     6181 gttaaaggcg gtcataaagg ccaccagcgc caccacaaac atccacgatg ggatggaagg
     6241 caccagagct tcaaaataga ttttcgccag cagaatgttg atcatcggcg cgaacagata
     6301 gtcgagcaga gaagaccaac ccaccataaa gccgacagtc gggctaatgg atttctgggc
     6361 gtaagtatat gcagagccag cagaaggata gcggcgaacc agcttcccgt agctcagagc
     6421 cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc ggcacatgac catccgtgag
     6481 gcctgaaacg ataccaaaag tatcaaacag cgtcatcggc tgcatatagg caagacccat
     6541 cattacaacc ggaactaacg taagcgtttt acgcaattcc acgcgagagg tgtttggagt
     6601 aacgttatgc gacatggtca ttctccttta cggtgataac cgtcgcgtaa gcaaaaaatt
     6661 gccccatttt tttggattcc tcagcgacaa caactgtcga tttttagtaa atatctatcc
     6721 ggtacgaagc ccggcctctt ggtatgaatg attggtttga agcaaaaaat aaccgacgct
     6781 gatgaaacgt cggtttttag tcattttttg acagcggcgc attgtgccta aatggggggg
     6841 aaatgacaag agaatgagag gcttgtcata ataatttttc tttaaatggc tgattttccg
     6901 tcatcagatt ttgcataaac accggcgatc gcactatttg ctaaaatttc atcccgccac
     6961 cagccgctgg caagtcctgc ggtctgttca ttccagccaa tccagacggg ttcaaagaga
     7021 gtttgggcga ccactttctt ctcgattaac gcgccactat cgagaaaacg ttgcgccaga
     7081 taacggggta aatagccgca gccgaggccg ctaatttgca gctccagctt ggttttaaaa
     7141 tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg aggccgtaga agcgcctgcc
     7201 tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa tgatacgacg gtttaatggt
     7261 tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa agacttgttc cagatcgccc
     7321 aaccgcgaga aaccaaactc actggaagat ggtggctcat gcatcgcgcc aacaataata
     7381 tccgctcgcc cctgagtcag ggcatcccat gaaccaccga gtacgccatt gataaacttc
     7441 aggcgcgtca cgttatgatg ttgataaaaa gcttcaatga gtggcgcaag cagagaaaaa
     7501 ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc agccttcatg cagtttgatc
     7561 gcctgctttt ccagttctcg cacggtatgc agaacttccc gccccttctc taataacatt
     7621 tttccggtac gggtgaattt agcgcggtga ccgctacgat caagcaattg aatattaagg
     7681 tcgctttcca gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta gagtttagcc
     7741 gacgccgccg caaaactgcc ttctttttct aaggcatcga ggatcatcaa cacatccagc
     7801 agtggtttca tacacgcccc cttgcagtgc atgcggcgtc ctgctggcgt gatcgtttac
     7861 tccagcggca ttaccagcgg atcgggatac tggtattcaa atcccagttc attacaaatc
     7921 cgactgccat caataatctt acctttgccg ctgtccagac tatttctgaa ttgcggaggt
     7981 tccagcccca gtaaacgggc catctgcgga tagaaaacat tacgcgcagg gtgagctggc
     8041 gcacatatat tatagatgtg tccgccttta ggtgcctgta acaacagagt gatagcgcca
     8101 ataacatctt ctaaatggac taaattaaca ccatgttcac catcaggcgc ggtttttccg
     8161 gcaaagaagc gtccgggatg acgccccggt ccgaccaggc ccgcaagacg cagaatatcg
     8221 accgaagtac cgggtaaatt gtgcagccag tcttcgagtt cttctaatac tcgtccactg
     8281 ttggtcaccg gattacgcgg ggtggtttct ttcaccgtgc cttgcgcgtc gccatagaca
     8341 gacgttgaac tggtaaaaat aattcgggga atacgatggg ccagcgcgct atccactaac
     8401 tcttgtaccg cttgtaaata gaactcatcg ccggggccgc tacgacgggc cggaagcgta
     8461 atgaccagcg catcggcatc catcagggca tccagatcgt cagaatcgca aactaactca
     8521 ggctccatgc gaagcagata gctatcaatg ccactcattc gggccgcttt gacaccatct
     8581 tgtgtggttt tactcccggt gacttgccag cctcgcgctg aaagtgacat cgccagcggc
     8641 atgcctaacc accctaaccc gacaattgcg acctttttca tccgttatct cctgatacac
     8701 cttagatcta taaggctacg ctagcgtatc aaaactgaca attcattcta tgaatgaaat
     8761 cgaaactgtt agcaaatata ccattgcctg atgcgctccg cttattagtc ctacaaaaca
     8821 tcaatatatt gaatttaggc aacatttgta gaccagataa ggcattcacg ccgcatccgg
     8881 caggaacaaa gcccacttta gttacagaac acaatgttct tgagggtttt aatgacatat
     8941 tttcacgatt acttttttaa cctagtcact cttagccccc tcctttcccc gctcattcat
     9001 taaacaaatc catcgccata aaatatataa aaaagccctt gctttctaac gtgaaagtgg
     9061 tttaggttaa aagacatcag ttgaataaac attcacagag acttttatga cacgcgttca
     9121 atttaaacag caccatcatc accatcatcc tgactagtct ttcaggcgat gtgtgctgga
     9181 agacattcag atcttccagt ggtgcatgaa cgcatgagaa agcccccgga agatcacctt
     9241 ccgggggctt ttttattgcg cggttgataa cggttcagac aggtttaaag aggaataaga
     9301 aaatgacaga caacactcgt ttacgcatag ctatgcagaa atccggccgt ttaagtgatg
     9361 actctcgcga attgctggcg cgctgtggca ttaaaatcaa tcttcacacc cagcgcctga
     9421 tcgcgatggc agaaaacatg ccgattgata ttctgcgcgt gcgtgacgac gacattcccg
     9481 gtctggtaat ggacggcgtg gtagatcttg ggattatcgg cgaaaacgtg ctggaagaag
     9541 agctgcttaa ccgccgcgcc cagggtgaag atccacgcta ctttaccctg cgtcgtctgg
     9601 atttcggcgg ctgccgtctt tcgctggcaa cgccggttga tgatgcctgg gacggcccgc
     9661 tctccttaaa cggtaaacgt atcgccacct cttatcctca cctgctcaag cgttatctcg
     9721 accagaaagg catctctttt aaatcctgct tactgaacgg ttctgttgaa gtcgccccgc
     9781 gtgccggact ggcggatgcg atttgcgatc tggtttccac cggtgccacg ctggaagcaa
     9841 acggcctgcg cgaagtcgaa gttatctatc gctcgaaagc ctgcctgata caacgcgatg
     9901 gcgaaatgga agaatccaaa cagcaactga tcgacaagct gctgacccgt attcagggtg
     9961 tgatccaggc gcgcgaatca aaatacatca tgatgcacgc accgacccaa cgtctggatg
    10021 aagtcatcgc cctgctgcca ggtgccgaac gcccaaccat tctgccgctg gcgggtgatc
    10081 aacagcgcgt agcgatgcac atggtcagta gcgaaaccct gttctgggaa acgatggaaa
    10141 aactgaaagc gctgggtgcc agttcaatcc tggtcctgcc gattgagaag atgatggagt
    10201 gatcgccatg agctttaaca caatcattga ctggaatagc tgtactgcaa agcaacaacg
    10261 ccagctgtta atgcgcccgg cgatctccgc ttctgaaagc attacccgca ctgttaacga
    10321 tattctcgat agcgtgaaag cacgcggtga tgacgccctg cgggaatata gcgcgaagtt
    10381 tgataaaacc acggttaccg cactgaaggt gtctgctgag gaaattgccg ccgccagcga
    10441 acgcctgagc gacgagctaa aacaggcgat ggcggtggca gtaaagaata ttgaaacctt
    10501 ccacactgcg caaaaactgc cgccggtaga tgtagaaacg cagccaggcg tgcgttgcca
    10561 gcaagtcacg cgcccggtag cttcagttgg gttgtatatt cctggcggct ccgccccgct
    10621 cttctcaacg gtattaatgc tggcaactcc ggcgcgtatt gcgggctgta aaaaagtggt
    10681 gttgtgctca ccgccgccga ttgccgatga gatcctttat gcggcgcagc tgtgcggtgt
    10741 gcaggacgtg tttaacgtcg gcggcgcaca ggccattgcc gcgctggcgt ttggtacgga
    10801 atctgtgccg aaagtggaca aaatcttcgg gccgggtaac gcctttgtca ccgaggcaaa
    10861 acgtcaggtg agccagcgtc tggacggtgc ggcgatcgat atgcccgcag gcccgtcgga
    10921 agtgttggtc attgccgaca gcggcgcaac gccggatttc gtggcttctg atttgctttc
    10981 tcaggctgaa cacggcccgg actcacaggt gattttactg acgcctgacg ctgatatggc
    11041 gcatcaagtt gccgaagccg tcgaacgcca gttagcagaa ctgccgcgtg ccgaaaccgc
    11101 ccgccaggca ctgaacgcca gccgcctgat cgtgactaaa gatttagcgc agtgcgtgga
    11161 gatctccaac cagtacggcc cggagcacct gatcattcag acccgcaacg cccgcgaact
    11221 ggtcgatggc atcaccagcg ccggttcggt atttcttggt gactggtcac cggaatcggc
    11281 aggtgattac gcctccggaa ccaaccatgt tctaccgact tacggctaca ccgccacctg
    11341 ttccagcctc ggactggcgg atttccagaa gcggatgacc gtgcaggaac tgtcgaaagt
    11401 aggtttctcc gcgctggctt cgaccattga aacactggcc gccgccgagc gcctgaccgc
    11461 ccacaaaaat gccgttactt tgcgtgttaa cgcccttaag gagcaagcat gagcaccgtg
    11521 actattaccg atttagcgcg tgaaaacgtc cgcaacctga cgccgtatca gtcggcgcgt
    11581 cgtctgggcg gtaatggcga cgtctggctg aacgccaacg aatatcccac agccgtggag
    11641 tttcagctta ctcagcaaac gctcaaccgc tacccggaat gccagccgaa agcggtgatt
    11701 gaaaattacg cgcaatatgc aggcgtaaaa ccggaacagg tgctggtcag ccgtggcgcg
    11761 gacgaaggta ttgaactact gattcgcgct ttttgcgaac cgggtaaaga cgccatcctc
    11821 tactgcccgc caacgtacgg catgtacagc gtcagcgctg aaaccattgg cgtcgagtgc
    11881 cgcacagtgc cgacgctgga aaactggcaa ctggacttac agggcatttc cgacaagctg
    11941 gacggcgtaa aagtggtcta tgtttgcagc cccaacaacc caaccgggca actgatcaat
    12001 ccgcaggatt ttcgcaccct gctggagtta acgcgcggta aggcgattgt ggttgccgat
    12061 gaagcctata tcgagttttg cccgcaggca tcgctggctg gctggctggc ggaatatccg
    12121 cacctggcta ttttgcgcac actgtcgaaa gcttttgctc tggcgggcct tcgttgcgga
    12181 tttacgctgg caaacgaaga agtcatcaac ctgctgatga aagtgattgc cccctacccg
    12241 ctctcgacgc cggttgccga cattgcggcc caggcgttaa gcccgcaggg aattatcgcc
    12301 atgcgcgaac gggtggcgca aattattgca gaacgcgaat acctgattgc cgcactgaaa
    12361 gagatcccct gcgtggagca ggtttttgac tctgaaacca actacattct ggcgcgcttt
    12421 aaagcctcca gtgcagtgtt taaatctttg tgggatcagg gcattatctt acgtgatcag
    12481 aataaacaac cctctttaag cggctgcctg cgaattaccg tcggaacccg tgaagaaagc
    12541 cagcgcgtca ttgacgcctt acgtgcggag caagtttgat gagtcagaag tatcttttta
    12601 tcgatcgcga tggaaccctg attagcgaac cgccgagtga ttttcaggtg gatcgttttg
    12661 acaaactcgc ctttgaaccg ggcgtgatcc cgcaactgct gaagctgcaa aaagcgggct
    12721 acaaactggt gatgatcact aatcaggatg gtctgggaac acaaagtttc ccgcaggcgg
    12781 atttcgatgg cccgcacaac ctgatgatgc agatattcac ctcgcaaggc gtacagtttg
    12841 atgaagtgct gatttgtccg cacctgcccg ccgatgagtg cgactgccgt aaaccgaaag
    12901 taaaactggt ggagcgttat ctcgctgagc aagcgatgga tcgcgccaac agttatgtga
    12961 ttggcgatcg cgcgaccgat attcaactcg ctgaaaacat gggcattaat ggtttacgct
    13021 acgaccgcga aaccctgaac tggccaatga ttggcgagca actcaccaga cgtgaccgtt
    13081 acgctcacgt agtgcgtaat accaaagaga cgcagattga cgttcaggtg tggctggatc
    13141 gtgaaggtgg cagcaagatt aataccggcg ttggcttctt tgatcacatg ctggatcaga
    13201 tcgccaccca cggcggtttc cgtatggaaa tcaacgtcaa aggcgacctc tatatcgacg
    13261 atcaccacac cgtcgaagat accggcctgg cgctgggcga agcgttaaaa attgccctcg
    13321 gcgataaacg cggtatttgc cgctttggtt ttgtgctgcc gatggacgaa tgccttgccc
    13381 gctgcgcgct ggatatctct ggtcgcccgc acctggaata taaagccgag tttacctacc
    13441 aacgcgtggg cgatctcagc accgagatga tcgagcactt cttccgttcg ctctcttaca
    13501 ccatgggcgt gacgctccac ctgaaaacca aaggtaaaaa cgatcatcac cgtgtagaga
    13561 gcctgttcaa agcctttggt cggaccctgc gccaggccat ccgcgtggaa ggcgataccc
    13621 tgccctcgtc gaaaggagtg ctgtaatgaa cgtggtgatc cttgatactg gctgcgccaa
    13681 cctgaactcg gtgaagtctg ccattgcgcg tcacggttat gaacccaaag tcagccgtga
    13741 cccggacgtg gtgttgctgg ccgataaact atttttaccc ggcgttggca ctgcacaagc
    13801 agcgatggat caggtgcgtg agcgcgagct gtttgatctc atcaaagcct gtacccaacc
    13861 ggtgctgggc atctgcttag ggatgcaact gctggggcgg cgcagtgaag agagcaacgg
    13921 cgtcgatttg ctgggcatca tcgacgaaga cgtgccgaaa atgaccgact ttggtctgcc
    13981 gctgccgcat atgggctgga atcgcgttta cccgcaggca ggcaaccgcc tgtttcaggg
    14041 gattgaagac ggcgcgtact tttactttgt tcacagctac gcaatgccag tcaatccgtg
    14101 gaccatcgcc cagtgtaatt acggcgaacc attcaccgcg gcggtacaaa aagataactt
    14161 ctacggcgtg cagttccatc cggagcgttc tggtgccgct ggcgctaagt tgctgaaaaa
    14221 cttcctggag atgtgatgat tattccggca ttagatttaa tcgacggcac tgtggtgcgt
    14281 ctccatcagg gcgattacgg caaacaacgc gattacggta acgacccgct gccgcgctta
    14341 caggattacg ccgcacaagg tgccgaagtg ctgcatttgg tggatctgac cggggcaaaa
    14401 gatccggcaa aacgtcaaat cccgctgatt aaaaccctgg tcgctggcgt taacgttccg
    14461 gtgcaggttg gcggcggcgt gcgtagcgaa gaagacgtgg cggcgttact ggaagctggg
    14521 gttgcgcgcg tggtggttgg ctccactgcg gtgaaatcac cagaaatggt gaaaggctgg
    14581 tttgaacgtt tcggtgccga tgccttagtg ctggcgctgg atgtccgtat tgacgagcaa
    14641 ggcaacaagc aggtggcggt cagcggctgg caagagaatt cgggcgtgtc actggaacaa
    14701 ctggtggaaa cctatctgcc cgtcggcctg aaacatgtgc tgtgtaccga tatctcgcgc
    14761 gacggcacgc tggcaggctc taacgtctct ttatatgaag aagtgtgcgc cagatatccg
    14821 caggtggcat ttcagtcatc cggcggcatt ggcgacatta acgatgttgc tgccctgcgt
    14881 ggcactggcg tgcgcggcgt aatagttggt cgtgcattac tggaaggtaa attcaccgtg
    14941 aaggaggcca tctcatgctg gcaaaacgca taattccatg tctcgacgtt cgtgatggtc
    15001 aggtggtaaa aggcgtacag tttcgcaacc atgaaatcat tggcgatatc gtgccgctgg
    15061 caaaacgcta cgccgaagaa ggtgcagacg aactggtgtt ctacgatatc accgcttcca
    15121 gcgatggccg tgtggtagat aaaagttggg tatctcgcgt ggcggaagtg atcgacattc
    15181 cgttctgcgt ggcgggtggg attaagtctc tggaagatgc cgcgaaaatt ctttcctttg
    15241 gcgcggataa aatttccatc aactcccctg cgctggcgga cccgacgtta attactcgcc
    15301 tggcggatcg atttggcgtg cagtgtattg tggtgggtat tgatacctgg tacgacgctg
    15361 aaaccggtaa atatcatgtg aatcaatata ccggcgatga aagccgcacc cgcgtcactc
    15421 agtgggaaac gctcgactgg gtacaggaag tgcaaaaacg cggtgccgga gaaatcgtcc
    15481 tcaacatgat gaatcaggac ggcgtgcgta acggttacga cctcgaacaa ctgaaaaaag
    15541 tgcgtgaagt ttgccacgtc ccgctgattg cttccggtgg cgcgggcacc atggaacact
    15601 tcctcgaagc cttccgcgat gccgacgttg acggcgcgct ggcagcttcc gtattccaca
    15661 aacaaataat caatattggt gaattaaaag cgtacctggc aacacagggc gtggagatca
    15721 ggatatgtta acagaacaac aacgtcgcga actggactgg gaaaaaaccg acggactgat
    15781 gccggtgatt gtgcaacacg cggtatccgg cgaagtgtta atgctgggct atatgaaccc
    15841 ggaagcctta gacaaaacca tcgaaagcgg caaagtcacc ttcttctcgc gcactaaaca
    15901 gcgactgtgg accaaaggcg aaacgtcggg caatttcctc aacgtagtaa atattgcgcc
    15961 ggactgcgac aacgacacgc tactggtact ggcgaatcct atcggaccaa cctgccacaa
    16021 aggcaccagc agctgcttcg gcgacaccgc tcaccagtgg ctgttcctgt atcaactgga
    16081 acaattgctc gccgaacgca aatctgccga cccggaaacc tcctacaccg ccaaactgta
    16141 tgccagcggc accaaacgca ttgcgcagaa agtgggcgaa gaaggcgtgg aaaccgcact
    16201 ggccgcgact gtacatgacc gctttgagct gaccaacgag gcgtctgact tgatgtatca
    16261 cctgctggta ttgttgcagg atcaggatct ggatttaaca acggtgattg agaacctgcg
    16321 taaacgtcat cagtgagttg cggggtaagc ggatgcgata ttgttgtcgc atccggcaaa
    16381 aaaaacgggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
    16441 cgttgtaatt acgcagagcg ttacgcccca gcacaatccc cgcgccaacc atgccgccca
    16501 gcagcaccgc cagaatcaag gtgattgcct ttttcggact atcgcgacga ataggtaacg
    16561 tcggtttcat tacatagcgg taagcatgaa tatcaagatc atcaaccttt aagttttcaa
    16621 tatccaacag gttttgacgt gtctgatagt agtttggtga gaacaccaac ggacgggtcg
    16681 cctcatgctt aatcatcgac tccagcgctt cgctccccaa aaggaacaac gtatcttgcg
    16741 tcacatcttc agtctgctga acctgtggct ttgccacctg cgcctgattc gcatactgca
    16801 acgcttcctg aatctgacgg atacgcagct ctttctgctc ctgcgcaact acttcctgcg
    16861 ttcttaaaga gtcctgcaag tttttccgtc ccagagcaat gttgtctttg agatcctttt
    16921 ctagctcttg attcacttta tcatcaactt gctgaatgta ttgggccaac ttcatttgtg
    16981 ctccctctgc agtttgccca acataagaaa cagtcaatgg taattgctgg ttcttaacag
    17041 aaggttcgat ggtaagtttt tctggttctt cctgattatc cagcgtttct gctaatgctg
    17101 agaaggcaga actgaagcga ccaatcaacg tctcctgcaa atccgatact ttcggtgcag
    17161 cctgaccata gataacattc atggcattgt tatagccagc aatttgcccc acgtcgggct
    17221 gagtgataat tgctgttgac gtccatttct ccttcgctac tgccaaataa ccaatagcca
    17281 gagcaatagc cacaatgacg gaaatgatga ttgtcatctt gccacgccac aactgcacta
    17341 gtaaatcaat caaatcaatc tgttccgggt catggttttg cccagaaaca ttattatttt
    17401 ctactctcat cattacccta actgacggaa atttccaagt ccgcatagtt tagcgataat
    17461 ccctgtgaat gctataggat aagtgataaa aataatgaat tattgcggag gtaacaaaag
    17521 acaatcaggg cgtaaatagc cctgataaca agatgttagt cgctgccaaa gagatcgcgg
    17581 gtgtagacct tatctgccac atccctaagc tcttctgcca tacggttgga aataatgacg
    17641 tcggcttgtt gtttgaaggt ggcgagatca cgttccaggc gagagttgaa gaatgagtct
    17701 tctttcatca ctggctcgta gatgattact tcaacacctt tcgccttgat acgcttcata
    17761 atcccctgaa tagaagacgc acggaagtta tctgaaccgc tcttcataat cagacgataa
    17821 atacctacca cttgcggctt gcgtgacaaa atggcatcgg caataaaatc tttacgcgtg
    17881 cggttagcat cgacaattgc cgagatcagg ttattcggca cagactggta gttcgccagt
    17941 aactgcttgg tatctttcgg caaacaataa ccaccataac caaacgacgg attgttgtaa
    18001 tggttgccaa tacgtgggtc gagacaaacg ccttcgatta tttgacggga attcagacct
    18061 aaactttctg cgtagctatc cagttcgtta aagtacgcca cgcgcatcgc gaggtaggtg
    18121 tttgcaaaaa gtttaatcgc ttctgcttca gtggagtcgg taaacagggt tgggatattt
    18181 tgcttaatcg cgccttcctg taataacgca gcaaaacgtt ctgcgcgttc cgaacgctca
    18241 ccgattacaa tacgtgacgg atgaaggtta tcgtaaagag ctttaccttc gcggagaaat
    18301 tccggagaga aaataatatt ttcagtacga tatttcttat gcatcgcttc ggtaaaacca
    18361 acgggaaccg ttgatttgat gaccataacc gcataaggat ttatctcaac tacgtcttta
    18421 attactgatt ctacactgga tgtattgaaa taattagttt taggatcata gtcggttgga
    18481 gtggcgatga tgacataatc agcatcccgg taggcttcat ttttatctaa tgtggcatta
    18541 aagtgtattt tatctgattg caaaaactgc tgaatttcct tatcaacaat aggagatatc
    18601 cgatcattca gcatagcaac gcgtgacggt aaaatatcta atgccacaac ctcatgattt
    18661 tgagcgatta gaagcccgtt tgacaagcct acatagccag taccggaaat ggtgattttc
    18721 atttcatgct ctcagaatta acttaactgt gaatcatgat gtttttaaca tcctgataag
    18781 agctaaaagt tttaacgcta cgttgttcga tggtcaacgc agcgaaaaac aattcagatt
    18841 ttctttataa gaatagctga tatttattaa gttaatatta agcagtgata tttagtctat
    18901 aagataattg gcaaaaaaaa gcccggtaca atatacgccg ggcctcaatt ttattgttga
    18961 ttaaatcaga ttaatccagc cattcggtat ggaacacacc ttctttatca atgcgcttat
    19021 aagtatgcgc accgaaatag tcacgctgtg cctggatcag gttcgcaggc agaacagcgg
    19081 cgcggtagct gtcataatag gcaaccgcag cggcgaaggt cggaaccggg ataccgttct
    19141 gtaccgcata agcgacgaca tcgcgcagcg cctgctggta gtcatcggca atttgcttga
    19201 agtaaggagc cagcagcagg ttagcgatct gcggattttc ggcataagca tcggtgattt
    19261 tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa aatcttcgcg atttcgccgt
    19321 agttcagatc ccagttgtac tcttcagacg ccgcacgcag ttgagagaac ccctgagcgt
    19381 aagaaacgat tttgcccaga tacagtgcac ggcgaacttt ttcgatgaac tcagccttgt
    19441 cgccagctgg ctgcgcttgt gggccagaga gaactttaga cgcagcaacg cgctgatctt
    19501 tcagagaaga gatgtatcgt gcaaacacag actcggtaat cagcgacagc ggttcgccga
    19561 gatccagtgc gctctggctg gtccatttgc ccgtaccttt gtttgccgct tcatccagga
    19621 tcacatcaac caggtagtta ccgtcttcat cttttttagt gaagatgtct ttggtaatgt
    19681 cgatcaggta gctgctcagt tcaccgttat tccactcggt aaagatctgc gccagttctt
    19741 cgttggtgag gttcagacca cctttaagca gagaataggc ttcagcaatc agctgcatat
    19801 cgccgtattc aataccgttg tgaaccatct tcacatagtg acctgcgcca tcggcaccaa
    19861 tataggtaac gcatggctca ccgtcttcag ccactgcggc gattttggtc aggatcggcg
    19921 caactagttc ataggcttct ttctgcccac caggcataat ggaaggacct tttagtgcgc
    19981 cctcctcacc accggaaaca ccggtaccga tgaagttaaa gccttctgca gaaagctcac
    20041 ggttacgacg aatggtgtcc tggaagaagg tattaccacc atcaatgatg atgtcacctt
    20101 tatcgaggta tggcttaagg gaatcaatag cagcatccgt gcctgcacct gctttcacca
    20161 ttaacaagat gcgacgaggc gtttccagag attcaacaaa ttctttcacc gtatagtaag
    20221 gaaccagttt cttgcctgga ttttcggcaa tcacttcttc cgtcttttca cgggaacggt
    20281 tgaaaataga gacggtataa ccacggcttt cgatgttgag cgcaaggttg cgccccatca
    20341 ctgccatacc gactacgccg atctgttgct ttgacattgt ttactcctgt caggggttac
    20401 cgctagatat gcggcttgaa taacgtataa aaatgtatca gagtattata aatttcatgc
    20461 tgtatcagta ataactaagt aaaggatagc ttaaaaagaa gccataattt agtaaattag
    20521 cgaaattata tatattttaa acattagtcc catttggaac gtgttttcgc attcccatta
    20581 aatagatgtt ggcgatcttt tcatttctct tgctggattt ccgcacacag ttataccggc
    20641 aggaacactt ttagtgacaa cagcccccat acctataatg gctcccgcgc caataataag
    20701 tgggcgatta ggaacaccct gcttaattac tgcacccgag cctatatatg cattgtcttc
    20761 aataacaaca tatccattac atttagcccc aggagcaaat gtaacatagt ctcctatttg
    20821 acaatcatgt gcaacgtatg agtatatgtt tgcatgaaaa aacctcccta tatgagtatt
    20881 agtagatatt gtaacaaagg gagaaataat agcgccacta cctatcatag tatgatcata
    20941 aacaacgcta tttggatgtt ttatagttat tggttcaacc ccgtgtaata atattgactc
    21001 agacactctc tgtcgtatct tatcattagc aatagcaaca ttaaaatact tttttaaata
    21061 aggggctttt agaaagcagt tggttgaaag cacttcaaaa ccattaactt tctttcctgc
    21121 caaaacatca tccacaaaaa ccagagcata gtcagaacca cattcttgct ttatttgttg
    21181 atttagaatg ggtattgttt ctctaccata acttccagca ccaaaaatac catacaaatt
    21241 cattttccca tctccaaaga aatatcttat caaaaccaat gcaagattat gaaagcccgc
    21301 acgctgcaat gcctgacgct aggaagtttc tgtccatgac tgcttttcgc atcttacatg
    21361 ataatcctgc cttgaataca ttatcattgt cagatattaa tagcgatgtg ccgcattcta
    21421 gaaaatcatt cgaatgcaac tcgccttctt atattatagt cgatttcatt cattaccaca
    21481 tcaggctact gtgcaactta ttctccatgc gccagtgatt tcgatttact gtggcgaact
    21541 tctctgcggt taaagcagca gatctaatat aatatttgat cgtcatttcg gattctttct
    21601 tttgctctgc tattattgag agaaagtgga ctgccattca taaattctgc agccctttcc
    21661 attcaaacgt gaaatcaata agctcatcag gagcatctca aacaatatga agacggacat
    21721 cgtctctgcc gtgcctcttt tcactaattg cgtaactgtc atgttttgga ttatttaatt
    21781 ctttcagcgt aaatatctcc tcaaagactc tattaagccg actcttattt ccttttacag
    21841 cgaataaata atcacatctc tgtttttata tcttctctgc aatatctttc tggcaagcca
    21901 tcgcatcagt tatgattatt tttcctttaa tacccatcat tttagaaagt tcatgaataa
    21961 ctgtgaacgc attaggtttc tcatccgtct tgatctatcc gaggaccaga ctgtgcattg
    22021 ctgaaacgca ctaatgacat gaaccactcc tctgcgacga ctcttgtcat aagtgtgccg
    22081 aagtgtttta ccatcaattg cgatgacgtc gttatcatct ggaaaatggc agacatgtat
    22141 tcagttaaca aagcactcgt gaaatattgc tggactgatg caggatacaa ctttggctat
    22201 aatatcgtaa acagggataa taaaaatcta ttgtctcaat tattttaagt tccattttgt
    22261 tctccttttt atataaggca catttgttat tacataagcg atgaaaaaat gtcttaagca
    22321 aaggaacgtc tcaatcgtga cgtgaaatgc aaattggtca gtacaaaact ttatttctat
    22381 taatcttttc ctagataaag ttgcggtata gtcgttatac ataaaaaagc ccacatattc
    22441 atcatatacc gcaataacat tactttaata taatcttaaa tacgcttaat gattaatgta
    22501 aataatcact cacttagcaa tttaagaaga gctttagttt tcttttccat tagctttaca
    22561 tcaccgcgtg attccacatt caaccgcacc accggttcgg tgttggagga gcgcaggtta
    22621 aagcgccagt cggcaaaggt catgctgatg ccatcggtgc gatccaccgc cagcgcctcg
    22681 cggctaaaat gctgttccac gcgattaatt gcctcaacgg gttgcgccag tttgctgttg
    22741 atctcaccgc ttgccggaaa cgccgccatc cggtcgcgca ccatttcgcc cagcgttttt
    22801 cctttcaggc acaccagttc ggcgaccagc agccacggga tcatgccgct gtcgcagtaa
    22861 gcgaaatcac ggaagtaatg gtgagcgctc atttcgccac cgtagatggc gtcttccttg
    22921 cgcatacgtt ctttaataaa ggcgtgtccg gttttcgaca ttaccggggt gccgcctgcg
    22981 gcagtcacca catcaacggt gttccaggag agacgtggat cgtggatgat cttcgcgccg
    23041 ggattttttt cgaggaacgc ttctgccagc aggccgacaa tgtagtagcc ctcgataaac
    23101 tgcccttttt cgtcaaacag gaaacagcgg tcaaaatcgc catcaaaggc aatgcccata
    23161 tccgcgccgt gtttgatgac cgcattacgg gtgtcgtcgc ggcattccgg cagcagcggg
    23221 ttaggaatac cgttggggaa attgccgtcc ggcgtgttgt gtactttgat taattccacc
    23281 ggtgcgccga gggctttaaa tcgggcttca atggcgtcca ccaccggacc cgctgcgccg
    23341 ttcccggagt tgatcaccag cttgagcggc gtgaggtttt tgacgttgat ataaccgaac
    23401 aggtgatcaa cgtaagcgtc acgcagattg atttgctgat agcgaccacg tttggtttca
    23461 tcgacaggag ggaagtcatt ggcttctgcc agacgctgga catcgcgcag tccggtatca
    23521 ccgctgatcg ggcgagcccc ttcgcgcacc agcttcatgc cgttgtaatc catcgggtta
    23581 tggctggcgg taacttcgat gccgccatcc actccgagat ggaacgtggc gaaatagatc
    23641 tcttcggtgc cggacatacc gatatccagc acatcgacgc ccgcatcctg taaacctttc
    23701 gcaagcgcca gttttaacgc ttcgctggtg aggcggacat caccgcctaa aacaatggtt
    23761 ttcggtttga gaaattcgcc ataggcacgc ccaatgcgcc aggcaatatc ttcattcagt
    23821 tcttcgccta atttcccgcg aatatcatag gctttaaagc aggttaaaga tttcatatgt
    23881 taatcttcat gtttgtaacg ttctttctgt cttataatat catcctctcc caaataatcc
    23941 cctgaactga cttcaataag attaagaggg attatgcccg gattctcaag actatacgct
    24001 gcgccaaggg gaatgtatat cgattcattt gcggtgacta gtttagtttt atcgccaagg
    24061 gttacttttg ctgtaccaga aagcacgatc caatgttcag aacgatggtg atgcatcctt
    24121 aaagaaagcc cctcaccagg tttcacaata attttcttga ctttgtatcg ctcaccttgg
    24181 tcaatcgaat caaattttcc ccatggtcgg aaaacttcac gatgactaat atactctgta
    24241 cgttgctgca atttaagcat ttcgactatt ttttttacat gctgtacatc actcttttta
    24301 gacacaagaa cggcatcttt agtttgcacg ataaccatat cttcaattcc aatggcggct
    24361 accaacgctg actcagagta gatataatta ttcttagtat cataggttaa tatatcacct
    24421 ttacatacat ctcctgtttt cgattttaga ctaatgtccc ataacgattg ccaagatcca
    24481 acgtcactcc aaccaatatc aacagggcat acaacacatt tttctgtttt ttccattaca
    24541 gcaaaatcaa tagattcagc aggacaatct tgaaattgtt cttttgataa tcgaataaaa
    24601 tctagatcaa tgtatgagga tgaggcaacc tgttcacaaa cattgtaaat gtcaggtcta
    24661 aattttctca attcctcaag ataaacagat gccttaaaca tgaatattcc actattccaa
    24721 tagtgattac cagaagtcat atacaattct gcggtttcac gattaggctt ttcgacaaac
    24781 ttatttacat aataaaaacc agtattttca tgcccttgaa gcggtacaga tagttcacct
    24841 ctctcaatat acccataacc agtttcagca tattctggta taattccaaa cgttacaatt
    24901 ttaccttgat tagcgatggg agttgcattt ttaatagcat cacagaaaac actttcttta
    24961 gctataacgt ggtctgccgc aagaactaga agcaatggat cttcctgagg attacgtttt
    25021 aacgcatgaa acgcagatat cgctattgct ggtgcagtat ttcgcccgca tggttctaga
    25081 ataatattac catttaattt atttatttcc cttaactgtt cagccacaac aaagcgatgc
    25141 tgttcatttg ttatcactaa tggtttttga catgataggc ctgaaagtcg tagcaaagtc
    25201 gtttgtaaca aggtgttatc accagagagt tgtaaaaact gctttggata tagttcacga
    25261 gaaagtggcc aaagacgact gcctgtacca ccggccataa ctacagcaat tattggcgca
    25321 tcagacatat tatatcctta ttatctaagc caatcgcatc atttgtttta ttgttaaaat
    25381 atgcgcgaca attataatgc acatcgtcat catttatcag ctttgctcgc gatagccagc
    25441 aataagcatt atgttgtgac ttcggtaatt tcaaaatgtt tggttgtaat ttaagaagga
    25501 atgctataac tatataatgc gttgaaaagt ctccccccat actattgtct tcgtagaagt
    25561 gctgccagat accataaaac ttacccacag agagaggcaa acgaattcct agttcaattt
    25621 ctgtcaatcg ttcaaaggct gtctgcaatt tttcatcttt caacacccta ccaccaggaa
    25681 cgaaccaata gccctgtgcc gggcggttga ttcgttttcc tagcaaaatt tcgccaaact
    25741 cgttttccac aatcaaatct atagaaataa gaggagtaga ccttacaatt gtggcaaagt
    25801 cttgggaatg taaaaacatt attacccccg atattgaagt tggttttcaa gaaaccagtt
    25861 gtatgtattt tcaagaccct tgtgaagggt aattttatga ttccaaccta gttgatgaag
    25921 aagcgttaca tcaagtagtt ttcgaggggc tccatcgggc tttgttgtat cgaacgtaat
    25981 atgcccttta taacctacaa cttttgctat tgtttccgca agctcacaaa tcgtgcagtc
    26041 aatacctgtt ccaatattga tatgagacaa cattacttta gtattttttt gccatatatc
    26101 gtatggcatc tccatgacat aaatgcttgc agaagccata tcatctacat gtaagaattc
    26161 acgctttgga gtaccacttc cccaaacaac aacattcgga gaattgtttt ccacagcatc
    26221 atgaaagcgg cgcaaaagcg ccggaatcac atgagaatta cttggatgaa aattgtcatt
    26281 tggaccataa agattggttg gcattactga acggtaatca cgcccaaact gacggttata
    26341 agattcacat aatttaatac ctgcaatttt tgcgatagca taaggttcat ttgttggctc
    26401 aagtttccct tgtaataatt cgtcttccat aatcggttgg tgtgctaact taggataaat
    26461 acacgacgaa ccgaggaaaa gcagtttatt tacattattt ttgtgggcag catgaatgac
    26521 attcgcctct atcattatat tctcatatat aaaatcggca ggataagaac tgttagctaa
    26581 aatacctccg acttttgctg ctgccaaata aacctggtcg attttctgtg aagaaaaaaa
    26641 atccaaaaca gcgctactat ccaacaagtt caattcatcc cgagtacgta aaaccaactc
    26701 aacatcatca cgttgtttga ggcgtcgggt aatagctgat ccaaccattc cttggtgacc
    26761 agcaataaaa atacgttgct tattcatcat cattccagag ctaagcttac agaaaaacca
    26821 tgcgatttta aaagagaatg ttttttagcg gcttcaagat ctttggcaac catttcagaa
    26881 atcatttcag caagagtaat ttctggtctc caaccaagtt tgagattagc tttgctcgga
    26941 tctccaagca aagtatcaac ttcagctggt cggaaataac gaggatcaac agcaacaatg
    27001 acatcacctg gtttcacacc tggagcatcc tgtccttcaa ccgaatctac aatgcctttt
    27061 tcttcgattc ctttaccaac aaagctcatc ttaataccaa gttgtgctgc tgccatttcg
    27121 acaaactgac ggactgagta ttggactcct gttgcaatca caaaatcttc gggttgctcc
    27181 tgttgtaaca tcaaccattg cattctaaca taatcttttg catgtcccca atctcgtaac
    27241 gaatccatat tccctaaata caaacatgat tccaagcctt gtgcaatatt tgcaagtcct
    27301 cgagtaattt tccttgttac aaacgtttct ccacggcgtg gagattcatg attgaacaat
    27361 ataccattac atgcataaat accatatgac tctcgataat ttaccgtgat ccaatatgcg
    27421 taaagttttg caactgcata aggggaacga ggataaaaag gggtggattc tttttgaggg
    27481 atttcctgaa caagtccata taattctgag gttgaagctt gatagaaacg cgttttgttt
    27541 tccaatccta aaaagcgaat tgcttccagt aaacgtaatg taccaattgc atcgacatcg
    27601 gctgtatatt ctggagactc aaaagaaact gctacgtgac tcatagcagc taaattatat
    27661 acttcatctg gctgtacctc ctttagaatt ctagtgaggt tagatgaatc agtcagatct
    27721 ccatagtgca agtgaaaatt tgggttagaa ccatgtggat cttgataaat atggtctatg
    27781 cgttctgtat taaaagatga ggctcggcgt ttgataccat gaacttcata ccctttatca
    27841 agcaaaaact cagctagata agatccatct tgtccagtta cacctgtaat aagagcgact
    27901 ttagtcatta tatacctctt gaatatatta agatcgggat tcaatttctt gtttcacaac
    27961 atctaaaaga aaatctgcac acgcattgcg tcttttgaga cccaattcta tcagagttgt
    28021 aggttcataa aaaattttag attcatcagc ttttaccatc gcgtcattta atgaataatg
    28081 gttttttgcc tgaaagaaat atacatcacc gcaattaact tctttattga catctatgtc
    28141 agatagtata acttttttcc ctaatgcaat agcgtcaaat gttacccccc ctccaggccc
    28201 gccttcaaat aaggttggtt gtattacagc aatgcaattt ttgattaatt caatttgttc
    28261 aagtttaggt atatgcccta atatcttaat tttcgattca attccgagct tttttgccaa
    28321 aaccat
//
LOCUS       DAZFBR010000060        28266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFBR010000060 DAZFBR010000000
VERSION     DAZFBR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.060"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..209)
                     /locus_tag="ACHJZ2_002772"
     CDS             complement(<1..209)
                     /locus_tag="ACHJZ2_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4568850.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHJZ2_002773"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHJZ2_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO4568851.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHJZ2_002774"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHJZ2_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO4568852.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHJZ2_002775"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHJZ2_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO4568853.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHJZ2_002776"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHJZ2_002776"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO4568854.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHJZ2_002777"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHJZ2_002777"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO4568855.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHJZ2_002778"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHJZ2_002778"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO4568856.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHJZ2_002779"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHJZ2_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4568857.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHJZ2_002780"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHJZ2_002780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4568858.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHJZ2_002781"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHJZ2_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO4568859.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHJZ2_002782"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHJZ2_002782"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4568860.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHJZ2_002783"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHJZ2_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO4568861.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHJZ2_002784"
     CDS             complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHJZ2_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO4568862.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHJZ2_002785"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHJZ2_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO4568863.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHJZ2_002786"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHJZ2_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4568864.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHJZ2_002787"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHJZ2_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO4568865.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHJZ2_002788"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHJZ2_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO4568866.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHJZ2_002789"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHJZ2_002789"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO4568867.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHJZ2_002790"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHJZ2_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO4568868.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHJZ2_002791"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHJZ2_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO4568869.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHJZ2_002792"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHJZ2_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO4568870.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHJZ2_002793"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHJZ2_002793"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO4568871.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHJZ2_002794"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHJZ2_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO4568872.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHJZ2_002795"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHJZ2_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO4568873.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHJZ2_002796"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHJZ2_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO4568874.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHJZ2_002797"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHJZ2_002797"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO4568875.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHJZ2_002798"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHJZ2_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO4568876.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHJZ2_002799"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHJZ2_002799"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO4568877.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHJZ2_002800"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHJZ2_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO4568878.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="ACHJZ2_002801"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="ACHJZ2_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO4568879.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cysG"
                     /locus_tag="ACHJZ2_002802"
     CDS             26708..28081
                     /gene="cysG"
                     /locus_tag="ACHJZ2_002802"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO4568880.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6833 a         7592 c         7135 g         6706 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcctgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatacgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgagc acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggccggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggggtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagatcca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gtagctgaaa
     4081 aacgccatca gcaataaaga agacggccag ctcgtcagtt aacgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgcc cggcagcggt accatgaggc acagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tccctgctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccattt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca agtggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaagg tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctgctcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagtggaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaagg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctact ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccgc aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct agcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc ataccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatctacct gcgtggcatc accgtaataa actttgtagc
     8161 catatttgcg catcaggtta acggcgctga tatcccgctc cagcacagta atgcgcattt
     8221 tatttgccat caacaaacga ccaatcacct gcccaaaacg tccaaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagtgacat cccgacagag ataaagaaca aaccgagcag caagccttta aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgcct gcaataaacg
     8761 taccgagcgc catcgacagc cccaacgcat ccataaacaa tgcggaaccc aataccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcaataa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gtaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcggtcgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcaagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcaggacat cgctcatcgg
     9661 gtaacgattc aacgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacact
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacggcttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 tggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gctgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgtcgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaat ctggtccccg gttcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagtggtg agataggcct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcgcct attcaacatc tggcacgttt agcgccgcag gaactggagc aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatc gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgaccttgat atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgcaa ggtgaaattc accccgtact ggcagcgact ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgagtttggc catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct cccggttgat
    12601 gcggcggtta ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc tgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagcc gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt acctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg attcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtgcccgg
    14161 cggcaaaatg ggtctggcaa ttgaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcaga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggctgtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctcaatc tggcaaaaca accagacgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc ttgaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atattaacta
    15721 tgcactgcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccttttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccga cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctattggc acttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcgct
    16741 gaaaagttat ttgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcttga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cgcaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga ccttgatgct gattaccacc cttgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggatccg caaccaacgc cactggcctg gcagcgaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaacca gcgttcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag tcagtttatt gttgaacata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat gcacgccgca tccgacatct
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggcgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg
    18001 cctgaccttt gtactgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattaatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacaact
    18421 cacgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgatggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacgc aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttatg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcattg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacttcaaag cacgctggta acgagtcagg ttccactacc
    19321 agcgaatgat agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gagatgacaa ctttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca acgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gttttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgttgttgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgact cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctgaaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgaaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcaccaaaaa cggatgctcc ggtagcgaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaat gatgctggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccggcac atatggcgtg gtcaattctc gccctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctcatgcc ggacggcact atcgtcgtcc ggccttttcc tctctaaccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aattaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcagaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgagagcatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgttgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagcaaag agatctacaa acgccttatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaatgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgtc acaccgttgc ggcgctgaaa
    24241 gccgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactatt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggttacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcgg taggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgtctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcggcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtactg ccgacaaact gacgcgtacc
    25141 gcaccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccagta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtcgcgatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg actgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagc gacggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc gcgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaataacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgcg tatgtgggtc ttgggatcat cctgattttt acgctcggta atttgctcga
    26041 tccatccgta cgccctctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgagctgt tcaccggaca caccatgttc ctcacctttg gggtgaaagc
    26161 gggcaccatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggt ggtggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgtctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgcaat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactccatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtggtcgatg cgccgaaagc tgccagcttc attatgccgt cgattattga
    27061 ccgctcaccg ctgatggtgg cggtttcctc tggcggtact tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gctgcatctg ggccaggtag cgaaatacgc
    27181 cgggcagtta cgcggtcggg taaaacaaca gttcgccacg atgggtgaac gccgccgctt
    27241 ctgggaaaaa ctgttcgtta acgaccgtct ggcgcagtcg ctggcgaaca acgatcagaa
    27301 agccattact gaaaccaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acactgaaag gtctgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgcggatc gtgttttcgt cggcaaacgc gcgggatacc actgcgtacc
    27541 gcaggaggag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggc ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctcagc
    27721 ctattcgggt attccgctca cgcatcgcga ttatgcccag agcgtgcgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggctga atcaggctgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tagcaattgt cgaaaacgga acagcagtca cgcagcgcgt
    27961 gattgacggt acgctcacac aactgggcga actggcgcag caaatgaata gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta
    28081 atttaatcag ggccagagaa ttctggcctt tttcgtttac aactctcttt atatattcag
    28141 cagataaaaa tataactcat taacaaaaat catttgtttt tgccccgtta tattcaccgt
    28201 tacagcgact tacaaaatta agtcattcac tcactgacgc cgattaaata ttcctgtatc
    28261 gttctt
//
LOCUS       DAZFBR010000061        28214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFBR010000061 DAZFBR010000000
VERSION     DAZFBR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.061"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..2015
                     /gene="yihQ"
                     /locus_tag="ACHJZ2_002803"
     CDS             <1..2015
                     /gene="yihQ"
                     /locus_tag="ACHJZ2_002803"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO4568881.1"
                     /translation="PDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSGIADIDMFR
                     GNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGRLLLELQND
                     NLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQTYVTWQAD
                     CKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALWEDKATLRF
                     ECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMRNAGVKVNG
                     IWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYINPYVASDK
                     DLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKKNMIELGCG
                     GWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEILFFMRAGS
                     TGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGYTTLFEMKR
                     SKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFTTLKPYLKE
                     AVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRSDWTLYLPE
                     DNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            2061..3446
                     /locus_tag="ACHJZ2_002804"
     CDS             2061..3446
                     /locus_tag="ACHJZ2_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4568882.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            3489..4892
                     /locus_tag="ACHJZ2_002805"
     CDS             3489..4892
                     /locus_tag="ACHJZ2_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4568883.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            4959..5651
                     /gene="ompL"
                     /locus_tag="ACHJZ2_002806"
     CDS             4959..5651
                     /gene="ompL"
                     /locus_tag="ACHJZ2_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO4568884.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(5742..7007)
                     /locus_tag="ACHJZ2_002807"
     CDS             complement(5742..7007)
                     /locus_tag="ACHJZ2_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4568885.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(7109..8089)
                     /locus_tag="ACHJZ2_002808"
     CDS             complement(7109..8089)
                     /locus_tag="ACHJZ2_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO4568886.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVREAYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKELLTHLICLGDEEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPEKHLLDERLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(8097..8807)
                     /locus_tag="ACHJZ2_002809"
     CDS             complement(8097..8807)
                     /locus_tag="ACHJZ2_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4568887.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(9024..10847)
                     /gene="typA"
                     /locus_tag="ACHJZ2_002810"
     CDS             complement(9024..10847)
                     /gene="typA"
                     /locus_tag="ACHJZ2_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO4568888.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            11220..12629
                     /gene="glnA"
                     /locus_tag="ACHJZ2_002811"
     CDS             11220..12629
                     /gene="glnA"
                     /locus_tag="ACHJZ2_002811"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO4568889.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            12803..13852
                     /gene="glnL"
                     /locus_tag="ACHJZ2_002812"
     CDS             12803..13852
                     /gene="glnL"
                     /locus_tag="ACHJZ2_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO4568890.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            13864..15273
                     /gene="glnG"
                     /locus_tag="ACHJZ2_002813"
     CDS             13864..15273
                     /gene="glnG"
                     /locus_tag="ACHJZ2_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO4568891.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            15385..15495
                     /locus_tag="ACHJZ2_002814"
     CDS             15385..15495
                     /locus_tag="ACHJZ2_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO4568892.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(15685..17058)
                     /gene="hemN"
                     /locus_tag="ACHJZ2_002815"
     CDS             complement(15685..17058)
                     /gene="hemN"
                     /locus_tag="ACHJZ2_002815"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO4568893.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(17247..17753)
                     /gene="yihI"
                     /locus_tag="ACHJZ2_002816"
     CDS             complement(17247..17753)
                     /gene="yihI"
                     /locus_tag="ACHJZ2_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO4568894.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(17872..18038)
                     /locus_tag="ACHJZ2_002817"
                     /pseudo
     CDS             complement(17872..18038)
                     /locus_tag="ACHJZ2_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18335..18967
                     /gene="yihA"
                     /locus_tag="ACHJZ2_002818"
     CDS             18335..18967
                     /gene="yihA"
                     /locus_tag="ACHJZ2_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO4568895.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(19349..22135)
                     /gene="polA"
                     /locus_tag="ACHJZ2_002819"
     CDS             complement(19349..22135)
                     /gene="polA"
                     /locus_tag="ACHJZ2_002819"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO4568896.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            22499..23431
                     /locus_tag="ACHJZ2_002820"
     CDS             22499..23431
                     /locus_tag="ACHJZ2_002820"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO4568897.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(23472..24902)
                     /locus_tag="ACHJZ2_002821"
     CDS             complement(23472..24902)
                     /locus_tag="ACHJZ2_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO4568898.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDSSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(25057..25683)
                     /gene="dsbA"
                     /locus_tag="ACHJZ2_002822"
     CDS             complement(25057..25683)
                     /gene="dsbA"
                     /locus_tag="ACHJZ2_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO4568899.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINTGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(25700..26686)
                     /gene="srkA"
                     /locus_tag="ACHJZ2_002823"
     CDS             complement(25700..26686)
                     /gene="srkA"
                     /locus_tag="ACHJZ2_002823"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO4568900.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(26763..27032)
                     /locus_tag="ACHJZ2_002824"
     CDS             complement(26763..27032)
                     /locus_tag="ACHJZ2_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO4568901.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            27102..27686
                     /gene="mobA"
                     /locus_tag="ACHJZ2_002825"
     CDS             27102..27686
                     /gene="mobA"
                     /locus_tag="ACHJZ2_002825"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO4568902.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            27668..28195
                     /gene="mobB"
                     /locus_tag="ACHJZ2_002826"
     CDS             27668..28195
                     /gene="mobB"
                     /locus_tag="ACHJZ2_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO4568903.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT         6915 a         7055 c         6821 g         7423 t
ORIGIN      
        1 taccagattt tgaatttcat cagaataacg acagttttac cctacatttt cgacaacgtc
       61 ttattttaac ccatagcaaa gataatcctt gtttatggat tggctcaggt atagcggaca
      121 tcgatatgtt ccgcggcaat ttcagcatta aagataaact acaggagaaa attgcgctta
      181 ccgacgccat cgtcagccag tcaccggatg gttggttaat ccatttcagt cgtggttctg
      241 acattagcgc cacgctgaat atctctgccg acgatcaggg ccgtttattg ctggaactac
      301 aaaacgacaa ccttaaccac aaccgcatct ggctgcgcct tgccgctcaa ccagaggacc
      361 atatctacgg ctgcggcgaa cagttttcct acttcgatct gcgcggcaaa ccgttcccgc
      421 tatggaccag tgaacaaggc gttggtcgca acaaacaaac ctatgtcacc tggcaggccg
      481 actgcaaaga aaatgcgggc ggcgactatt actggacttt cttcccacag cctacgtttg
      541 tcagcacgca gaagtattat tgccatgttg ataacagttg ctatatgaac ttcgacttta
      601 gtgccccgga ataccatgaa ctggcgctgt gggaagacaa agcaacgctg cgttttgaat
      661 gtgctgacac atacatctcc ctgctggaaa aattaaccgc cctgctggga cgccagccag
      721 aactgcccga ctggatttat gacggagtaa cgctcggcat tcagggcggg acggaagtgt
      781 gccagaagaa actggacacc atgcgtaacg cgggcgtgaa ggtcaacggc atctgggcgc
      841 aggactggtc cggtattcgt atgacctctt ttggcaaacg cgtgatgtgg aactggaagt
      901 ggaacagcga aaactacccg caactggatt cgcgtatcaa gcagtggaac aaagagggcg
      961 tgcagttcct tgcctatatc aacccgtatg ttgccagtga taaagatctc tgcgaagagg
     1021 cggcaaagcg cggttatctg gcaaaagatg tcgccggtgg cgactatctg gtggagtttg
     1081 gcgagttcta cggcggcgtt gtcgatctca ccaatccgga agcctacgcc tggttcaagg
     1141 aagttatcaa aaagaacatg attgaactcg gctgcggcgg ctggatggct gacttcggcg
     1201 agtatctgcc caccgacacg tacctgcata acggcatcag cgcggaaatt atgcataacg
     1261 cctggcccgc gctgtgggcg aaatgtaact acgaagccct tgaagaaacg ggcaagctcg
     1321 gcgagatcct cttctttatg cgcgccggtt ctaccggtag ccagaaatac tccaccatga
     1381 tgtgggcggg tgaccagaac gtggactgga gtctggacga tggcctggcg tcggttgtcc
     1441 cggcggcgct atctctggca atgaccggac atggcctgca tcacagcgac attggcggtt
     1501 ataccaccct gtttgagatg aagcgcagca aagagctgct gctgcgctgg tgcgatttca
     1561 gcgccttcac gccgatgatg cgcacccacg aaggtaaccg tcctggcgac aactggcagt
     1621 ttgacggcga cgcggaaacc atcgcccatt tcgcccgtat gaccaccgtc ttcaccaccc
     1681 tgaaacctta cctgaaagag gccgtcgcgc tgaatgcgaa gtccggcctg ccggttatgc
     1741 gcccgctgtt cctgcattac gaagacgacg cgcagaccta ttccctgaaa tatcagtacc
     1801 tgttaggtcg cgacattctg gttgctccgg tacacgaaga aggtcgcagc gactggacgc
     1861 tctatctgcc agaggataac tgggtccacg cctggaccgg agaaaccttc cacggtggag
     1921 aaataactgt tgaagcgccc atcggcaagc cgccggtctt ttatcgcgcc gacagcgaat
     1981 gggctgcact gttcgcctcg ttaaaaaaca tctaatcatg cccgcccgtg gggaaactca
     2041 cgggcaaatg gagaataacg atgagtcaca tcacaacgga agatccggca actttgcgcc
     2101 tgccctttaa agagaaactc tcttacggta tcggcgatct ggcctctaac atcctgctgg
     2161 atattggtac gctttatctt ttgaagtttt ataccgacgt tctggggcta cctggcacct
     2221 atggcggcat tatctttttg atctcgaaat tctttaccgc ctttaccgat atgggaaccg
     2281 gcatcatgtt ggattcccgg cgtaagattg gcccgaaagg taagttccgc cctttcattt
     2341 tgtacgcgtc attcccggtc accttattgg caatcgctaa ctttatcggc acaccgttcg
     2401 atgtcactgg taaaacggtg atggccacta ttctgtttat gctctacggg ctgtttttca
     2461 gcatgatgaa ctgctcctat ggcgcgatgg tgcctgctat taccaaaaac cccaacgagc
     2521 gcgcatcact ggcggcatgg cgtcagggag gcgctacgct gggcctgctg ctgtgcacgg
     2581 tgggattcgt gccggttatg aatcttatcg aaggtaatca gcaacttggc tatatcttcg
     2641 ccgccacgct gttttcactg ttcggcctgc tgtttatgtg gatctgctac tcgggcgtga
     2701 aagagcgtta tgtcgaaacc caaccagcca atccggcgca aaagcctggc ctgctgcaat
     2761 ctttccgcgc aattgccggt aaccgcccac tgttcattct gtgcattgcc aacctctgca
     2821 ctttaggggc gtttaacgtc aagctcgcca tccaggtcta ttacacccag tacgtactta
     2881 acgatcccat cctgttgtca tatatgggat ttttcagcat gggctgtatt ttcatcggtg
     2941 tgttcctgat gcctggcgca gtcaggcgtt ttggtaagaa gaaggtctat atcggcggcc
     3001 tactgatttg ggtgctgggc gatctgctca actatttctt cagcggcggt tcggtcagct
     3061 tcgtggcgtt ctcctgcctg gcattcttcg gctcagcgtt tgttaacagc ctgaactggg
     3121 cgctggtttc cgacaccgtc gagtacggcg agtggcgtac cggcgttcgt tcggaaggaa
     3181 cggtctacac cggcttcacc ttctttcgca aagtgtctca ggcgctggct ggtttcttcc
     3241 ccggctggat gctgacgcaa atcggttatg tgccgaacgt ggcgcaggct gaccacacca
     3301 ttgaagggtt gcgccagctg atcttcatct acccaagcgc actggcggta gtcaccattg
     3361 tggcgatggg ctgcttctac agcctgaacg agaagatgta tgtccgcatt gttgaagaaa
     3421 tagaagcccg taaacgcacg gcgtaataat aataaacaat gccctgcggg gcgttataag
     3481 gagtgattat gtctgaccat aatccactga cattaaaact gaatctgcgg gaaaaaatcg
     3541 cctatggtat gggcgacgtt ggttcgaatt taatgcttag catcggtact ctgtatctcc
     3601 tcaaatttta taccgatgaa ttagggatgc cagcttacta tggcgggatt atctttctgg
     3661 tggcgaagtt ttttaccgcg tttaccgata tgctcaccgg atttttactc gattcgcgga
     3721 aaaatattgg gcctaaaggc aaatttcgtc cctttatttt atacgcagcg gttccggcgg
     3781 cgttaattgc aacgctccag tttatcgcca ccacttttag tttgccggtt aaaacgacga
     3841 ttgccaccgc gctgtttatg atgtttggac tttcatacag tctgatgaac tgctcgtatg
     3901 gtgcgatgat cccggccatt actaaaaacc cgaatgagcg cgcgcagctc gcggcttacc
     3961 gccagggtgg tgcaaccata gggctattga tttgtaccgt ggcgtttatt ccgttgcagt
     4021 cgcttttttc tgactcaacc gtcggttatg cctgtgcggc actgatgttc tccattggtg
     4081 gctttatttt tatgatgctg tgctacagag gcgtcaaaga gcattatgtg gacacagcac
     4141 caaccggaca taaagccagt attctcaaat ctttttgcgc gatatttcgg aatccgccat
     4201 tgctggtttt atgcattgct aacttgtgta ccctggcggc atttaatatc aaactggcga
     4261 ttcaggtcta ttacacccag tatgtgctga atgatattaa tttattgtcg tggatgggat
     4321 tcttcagtat gggatgcatc ctcgtcggcg tattactggt gccattaact gtaaaatgtt
     4381 ttggtaaaaa acaggtttat ttagctggca tggtgctatg ggcggttggt gatatcctga
     4441 attatttctg gggaagtaac tctttcactt tcgtcatgtt ctcttgtatc gccttttttg
     4501 gcacggcctt tgtaaacagc ctgaactggg cactggtgcc agataccgta gattacggtg
     4561 aatggaaaac cggtattcgt gccgaaggtt ctgtttatac cggttatacc ttctttcgta
     4621 aaatttctgc cgcacttgct ggcttcttgc caggcattat gctgacgcaa atcggttatg
     4681 ttcccaacat cgcgcaaagt gatgcgacat tacagggttt gcgtcagctc atttttatct
     4741 ggccttgtgc actggcaatt attgctgcat tgacgatggg attcttttac acactcaatg
     4801 aaaaacggtt tgcgttgatt attgaggaaa tcaaccaacg caaaaataaa gagatggcga
     4861 cagaagaaaa aacggcttcc gtaacgttat aaatgtcaat agccgctatt tccatcctgg
     4921 tgggtggcgg cctccctacg tttaaaaatg gacttattat gaaaaatatt aatgcaataa
     4981 ttttattatc ttctctgacc tctgcctcag tattcgctgg cgcatacgtc gaaaacaggg
     5041 aggcatataa tctcgcctcc gatcagggcg aggttatgct acgtgtgggt tataacttcg
     5101 atatgggcgc gggtattatg ttaaccaata cctacacctt tcagcgagaa gatgaactaa
     5161 aacatggata taacgaaatc gaaggttggt atccgttatt taaaccaacc gataaattaa
     5221 ccatccagcc cggtggctta attaatgata agagtatcgg ttcgggtggt gctgtttatc
     5281 tggatgttaa ctacaaattt actccgtggt ttaacctgac agtgcgcaac cgttttaacc
     5341 ataataacta cagctcaacc gatttaaacg gtgatctgga taataatgac agctacgaga
     5401 ttggcaacta ctggaacttt attattaccg ataagttctc atataccttt gagccgcatt
     5461 atttttataa cattaatgat ttcaatagcg gcaatggcac gaagcatcac tgggaaatca
     5521 ctaacacctt ccgttaccgc attaacgagc actggctgcc ctatattgaa ttgcgttggt
     5581 tagatcgcaa cgtggaaccg taccaccgcg agcaaaacca gattcgtatc gggacgaagt
     5641 atttcttctg atgtgaaact gccggatgcg gcgtaaacgc cttatccggc ctacggttga
     5701 tacggttttc gttggccgga ttaagtacat ccggccatta attaagcttc agccgccagc
     5761 atctctttct tacgttggct aatacgctta accagcatta cggaaaccac cgcaccacag
     5821 aatgcgaagc aggccataat gccaaacacg attttgtagc caataatccc cggatttaaa
     5881 tccagaatgt agccatacag actgaagcag aacatcgccg gggcgtaacc aataaagcta
     5941 cccaacgcca tcgccgcgcc tgttttattt tcagcaattt ttgcttcgcc gataggtgca
     6001 aaaaatacgg ctcgctgtgt aaagactatc gcgccaaagc ccagcgtaca tgccatccct
     6061 aaatagaccg gcatactttc gtgcggcagc ataatcaaca gtacgagcgc agcggtactg
     6121 atgataaagg tgtagcatag atatttactc ggcgatttca ggattttatc tgaaatcatg
     6181 ccaccaatcg gtccaccaat cattttcaga cagtattggt taatgatgcc gtaagccccc
     6241 accagcgcaa cgggcaatag atagatgttt ttcaggaatg gaatgaagaa tgtcaggcca
     6301 cagtaaaccg cgtaaacgaa gaagacgttg aaagcgataa gccagatagt tttgtctttc
     6361 agcaccgagg tcatactggt gttttgcgat gccccgtctt cttttttcac ctcaacggac
     6421 ggtgcctctt ctttgtcatt caggacaaag aaaatagtaa tgcctacggc aatcacaata
     6481 agggaataga accagatgcc tgctttaaaa cctaataagc cactgccaaa ccaggtaaat
     6541 actgccaacg cagaaaatgc caccacggta tcgacaatgc cacgccctgt ttcgaagaag
     6601 ccaaacaacc gaccttgttg ttcgctattg cccaatcgac ttaccgactt gagcaagacc
     6661 ggccagttca tcatgtcgca agtaacgcca aatagcgccc agacaaagag gatgccccaa
     6721 taccccggca ttgtcgtcaa ataaacaccc agtaatcctg tcgcaatgag tgagaatgac
     6781 atggtgtatc tgcgcggtag tttatcggca aaataaatag acagaaaaaa gcccactgtg
     6841 gtaacaaatg agtttaccga catagcatta ccaatttgac cattggtcaa atggaaatat
     6901 tcctgcatcg ggatataaaa cgcatctttc agcgacggta atttataaat tgtaccgcca
     6961 cacagtgtta atagactaaa taacgcccat ttctttttcg tgagcataac atcccctaag
     7021 cgattttaaa aatcactgtt ttaaaggcaa taagataccg accattctta tggaatggtt
     7081 ttattaactg cgtatgagtt aaaacttgtt agtggttcag aagatggctc gcctcttgtt
     7141 tgatttgttg tagtacgtta cgcacatgat ttatctgatt aattgcatct tctttttctt
     7201 tacgtaaccg ctcatccagc aaatgctttt ctggcaatgg tgtttcactc atctggcgat
     7261 aagggatcca cggattatca tcttcgtctt caaagcggaa cgctggagca taataatcaa
     7321 cctcttcttc caggccatat gccgtcacct gcggttcttc atcgccgagg cagataaggt
     7381 gtgttaataa ctctttgaac ggcatatctc cctgacctga aatacaggct ttatgaccca
     7441 ggccaccttg ttctttaacg atcaaggcat ctttgatatg gacctgggta atatgcggtg
     7501 ccatcgtttt taaggcgtct atgggatgct catttgcatt gatcatattc gcaaaatcaa
     7561 acagtaagga aagagactcc atctcacttt ctttgaccag cgacaccaac tcatgacttt
     7621 ttaaatcttc atgctgttcg atcgtaaaag tcaggccgct atcctgatac gcttcccgca
     7681 cataggcaat gtcgttagcg atgatcgata atacgtcgcg caaattacct tcataacgtg
     7741 gataaaaacg tacggacgat gccccagttt tcaacgcgat ggcgacggct tcgtcaatag
     7801 atgccttatc tgaggcgctg gtttcaatat gaatatcaag gttcagacgg cgggctttat
     7861 ccccaaaggc agagagttct ttatcgtcca tattaccaag agaaaaacgc tcgccatcca
     7921 gaacatggat tttgacgcca cgtagattat tttcacttgc gatatcaagt aaatcagcag
     7981 gcaacactct ttccagccgc atatttaaat gaaaggcata agcatgtaaa taaagaggaa
     8041 gagtgtcgac acgttgtaga atctttcttg cgttatttat tgtcaccata aaacacctac
     8101 agattaatga tgaggtctac ggcctcaaat cgccaatatt caatatctat ctggacaata
     8161 ttgccttgct ggtcggcgcg atgcttttca attaagatcg ctggcatacc cgcagaaccg
     8221 ccaagatact tgctgatatc gcccggcatt ctgacgggtt tgaaaatcaa tttttttact
     8281 accgtctctt tttggtagca cttttcccag actgcagaaa atgagtggtt ttcaagttgt
     8341 tcaataaaac ccggagcaac ttcaggatta atatatgttt cgtgatagaa aactttatgt
     8401 ccttccagcg ccccccagcc ggtgatgcga tagagttcag ttgatggtgt cactgcaatc
     8461 agtggcgcga gcgtctcggg aatatcgctg gtacggtttt tctcggtaaa cccccaggaa
     8521 ggctctcgcc cttgctcaat tgcggcccgc tgaaagctcg ccgacagctc cggactgtaa
     8581 ttaaatcgcg gctgggtcac gaaccacccc ttacgatcct tacggaagat tttggattct
     8641 gcctcgaggt tcaacaacgc ctggcgcagc gtcatacgtt taatgcccag cagttctccc
     8701 agctctcttt ctgaagggag ttttccaccc ggcgtggtga tgccatcttt caaccaccga
     8761 tccagtttct cttttgcatt ttctacggtg gattgattct ccgccattgt ttaatccttt
     8821 tggtttaaac cagatgaagc atcttacgct tacgccaaaa aagaaagcaa ttaacggcgt
     8881 atgactttgc agataattgt gacaaagctc tctattgacg taaccacaga cttataaggg
     8941 agatactcgc attggtgtgc gctcacgaca ggcaaaaaaa aacctgccat cgctggcagg
     9001 ttttttatga ctaaaaaacg aaattaatcg tctttcggtg cgcggttggc gcgacgacga
     9061 tcgttttccg tcaggtgacg tttacgaata cggatagagg tcggagtcac ttctaccagt
     9121 tcgtcatcat cgatgaactc cagagcttgt tccagagtca tgcggatagg cggaaccaga
     9181 acaacggctt cgtcagtacc ggaagcacgc atgttggtca gtttcttacc ggtcaggcag
     9241 tttacagtca ggtcgttaga gcggctatga ataccgataa tctgaccttc gtaaacttct
     9301 gcaccgtgac cgaggaacag cttaccgcga tcctgcaaac cgaacagcgc gaacgcgacc
     9361 gctttaccct gaccgttaga gatcagtacg ccgttctgac gctgacccac ttcacccgga
     9421 cgcacgtcgt cgtagtggct gaaggtggag tacagcagac ccgtaccgga agtcatggtc
     9481 atgaactcag aacggaagcc aatcagacca cggcttggga tcacgtagtc gagacgtacg
     9541 cggcctttac cgtctggatt catgtttttc aggtcgcctt tacgttcgcc cagcgcctgc
     9601 attacagaac cctgatgctg ttcttcaacg tccagagtca cgttttcata cggctcttgt
     9661 ttacgaccgt cgatttcacg gaagataact ttcggacggg ataccgccag ttcgaaacct
     9721 tcacgacgca tgttttcgat cagaacagac aggtgcagtt cgccacgacc agaaacgcgg
     9781 aacgcatcgg cgtcttcggt ttcttctacg cgcagcgcaa cgttgtgtac cagttctttg
     9841 ttcagacgat ccaggatctg acgagacgtt acgaacttac cttctttacc gcagaacggc
     9901 gaggtgttaa cgcagaagaa catagaaacg gtcggctcat caacggagag tgccggcagc
     9961 gcttcaacgt tttgcgtgtc gcaaacggtg tcagaaatgt tcagttcgcc caggcccgtg
    10021 atcgccacga tatcgccagc ttccgccaga tcggtttcga tacgttccag accgaggtgg
    10081 cccagcactt taccaacttt cgcgttgcgg gttttgcctt cgctatcgat gatagtaacc
    10141 tgctggttcg gcttcacttt accgcgcttg atgcggccaa tgccgataac gccaacatag
    10201 ctgttgtaat cgagctgaga aatctgcatc tggaacggac cgtcaaggtc aacgtccggc
    10261 gcaggaacgt ggtcaacaat cgcctggtac agcggggtca tgtcttccgc catatcttcg
    10321 tggtccagac ccgcgatacc gttcagcgca gaagcgtaaa cgatcgggaa gtccagctgc
    10381 tcgtcggtcg cgtcgaggtt aacgaacaga tcgaatacct gatccacaac ccaatcagga
    10441 cgcgcgccag ggcggtcaac tttgttgata acaacaatcg gcttcaggcc gtaagcaaac
    10501 gcttttttgg ttacgaagcg cgtttgcggc atcgggccgt caaatgcgtc aaccaccagc
    10561 agcactgagt ctaccatgga cattacacgt tcaacttcac caccgaagtc ggcgtgcccc
    10621 ggggtatcaa cgatgttgat acggtaatca ttccatttga tagcggtgtt tttcgcgagg
    10681 atggtaatcc cacgctcttt ctccaaatcg ttggagtcca tcacgcgctc ttgggtttcg
    10741 gcacgagagt cgaacgtacc ggattgttgg agcagcttgt ctaccagggt ggttttacca
    10801 tggtctacgt gcgcgatgat ggcgatatta cgcaattttt cgatcacaac tttgcctcag
    10861 gcattagaaa tagcgcgtta ttgtacacgg attaatcgca ctacaaaaca ggatcacaaa
    10921 catcctccgc aaacaagtat tgcagagtcc ctttgtgatc gctttcacgg agcataaaaa
    10981 gggttatcca aaggtcattg caccaatatg gtgcttaatg tttccattga agcactatat
    11041 tggtgcaaca ttcacatcat ggtgcagccc ttttgcacga tgttaagcat gataacgcct
    11101 tttaggggca atttaaaagt tggcacagtt ttcgctttat cttttttacg gcgacacggc
    11161 caaaataatt gcagatttcg ttaccacgac gacaatgacc aatccaggag agttaaagta
    11221 tgtccgctga acacgtactg acgatgctga acgagcacga agtgaagttt gttgatttgc
    11281 gcttcaccga taccaaaggt aaagaacagc acgtcactat ccctgctcat caggtgaatg
    11341 ctgaattctt cgaagaaggc aaaatgtttg acggctcctc gattggcggc tggaaaggca
    11401 ttaacgaatc cgacatggtg ctgatgccag acgcatccac cgcagtgatt gacccgttct
    11461 tcgccgactc caccctgatt atccgttgcg acatccttga acctggcacc ctgcaaggct
    11521 atgaccgtga cccgcgctct attgcgaagc gcgccgaaga ttacctgcgt tccactggca
    11581 ttgccgacac cgtactgttc gggccagaac ctgaattctt cctgttcgat gacatccgtt
    11641 tcggatcatc tatctccggt tcccacgttg ctatcgacga tatcgaaggc gcatggaact
    11701 cctccaccca atacgaaggt ggtaacaaag gtcaccgtcc ggcagtgaaa ggcggttact
    11761 tcccggttcc gccggtagac tctgctcagg atattcgttc tgaaatgtgt ctggtgatgg
    11821 aacagatggg tctggtggtt gaagcccatc accacgaagt agcgactgct ggtcagaacg
    11881 aagtggctac ccgcttcaat accatgacca aaaaagctga cgaaattcag atctacaaat
    11941 atgttgtgca caacgtagcg caccgcttcg gtaaaaccgc gacctttatg ccaaaaccga
    12001 tgttcggtga taacggctcc ggtatgcact gccacatgtc gctgtctaaa aacggcgtta
    12061 acctgttcgc aggcgacaaa tacgcaggtc tgtctgagca ggcgctgtac tacattggcg
    12121 gcgtaatcaa acacgctaaa gcgattaacg ccctggcaaa cccgaccacc aactcttata
    12181 agcgtctggt cccgggctac gaagctccgg taatgctggc ttactctgca cgcaaccgtt
    12241 ctgcgtctat ccgtattccg gtggtttctt ctccgaaagc acgtcgtatc gaagtacgtt
    12301 tcccggatcc ggcggctaac ccgtacctgt gctttgctgc cctgctgatg gccggtcttg
    12361 atggcatcaa gaacaagatc catccgggcg aagccatgga caaaaacctg tatgacctgc
    12421 cgccggaaga agcgaaagag atcccacagg ttgcaggctc tctggaagaa gcactgaacg
    12481 agctggatct ggaccgcgag ttcctgaaag ccggtggcgt gttcacggat gaagcgatcg
    12541 atgcgtacat cgctctgcgt cgcgaagaag atgaccgcgt gcgtatgact ccgcatccgg
    12601 tagagtttga gctgtactac agcgtctaat agttgaagtt gtactacccg gcgcaacaac
    12661 gccgggattt agttgccgtg gaaactttca gcccatctct gcatgggctt ttttctccgt
    12721 caattctctg atgcttcgcg ctttttatcc gtaaaaagct ataatgcact aaaatggtgc
    12781 aacctgttca ggagactgct ttatggcaac aggcacgcag cccgatgctg ggcagatcct
    12841 caactcgctg attaacagta ttttgttaat cgatgacaac ctggcgatcc attacgccaa
    12901 ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa ttgtttggta cgccgttacc
    12961 ggaactgttg agctacttct cattaaatat tgagctgatg caagaaagtc tggaggcggg
    13021 gcaaggtttt accgataacg aagtgacgct ggtcatcgac ggtcgctcgc atatcctttc
    13081 tgtgacggcc cagcgtatgc cggacggcat gatcctgctg gagatggcgc cgatggataa
    13141 ccagcgccgc ttaagccagg aacagctaca acatgctcaa caagttgctg cccgtgattt
    13201 agtgcgtggc ctggcacatg agattaaaaa tccgcttggc ggtttacgtg gcgcggcgca
    13261 gctactcagc aaagcattac ctgacccgtc actgctggaa tataccaaag tgattatcga
    13321 acaggcggac cggctgcgaa atctggtcga ccgtctgttg gggccgcagc tgcccggtac
    13381 gcgcgttacc gaaagtattc acaaagtagc tgaacgcgtg gtaacgctgg tgtcgatgga
    13441 actgccggac aacgtgcggt tgattcgtga ttacgatccc agcctgccgg aactggcgca
    13501 cgacccggat caaattgaac aggtcttgct gaatattgtg cgcaatgcgc tacaggcgct
    13561 ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc gcgtttcaac tgaccttaca
    13621 cggcgaacgc tatcggctgg cggcgcggat tgatgtggaa gataacgggc caggcattcc
    13681 gcctcatttg caggatacgc tgttttaccc gatggtcagc ggccgcgaag gtggcaccgg
    13741 gcttggctta tccatcgctc gtaatttgat tgatcagcat tcaggcaaaa ttgaatttac
    13801 cagttggcca gggcataccg agttctcggt ttacctgcct atcaggaaat aaaggtgacg
    13861 tttatgcaac gagggatagt ctgggtagtc gatgacgata gttccatccg ttgggtgctt
    13921 gaacgtgcgc tcgctggagc gggtttaacc tgtacaacat ttgagaacgg cgcggaggta
    13981 ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt cagatatccg tatgccggga
    14041 atggacgggc tggcactgct caagcagatt aaacagcgcc atccgatgct tccggtcatc
    14101 attatgaccg cacattccga tctggatgct gccgtcagcg cctatcaaca aggggcgttt
    14161 gattatctgc ccaaaccgtt tgatatcgac gaagccgtgg cgctggttga gcgcgccatc
    14221 agtcattacc aggaacagca gcagccgcgt aatgttcagc ttaacggccc aacgaccgat
    14281 atcatcggcg aagcgccagc catgcaggac gtgttccgta ttatcggtcg gctttcgcgt
    14341 tcttctatta gcgtgctgat taacggcgaa tccggcaccg gtaaagaact ggtcgctcat
    14401 gccctgcatc gccacagtcc gcgagccaaa gcgccattta tcgctctgaa tatggcggct
    14461 atcccgaagg atttgatcga atcagaactg tttggtcacg agaaaggcgc gtttaccggc
    14521 gcgaatacca ttcgtcaggg gcgttttgaa caggctgatg gcggtacatt atttctcgat
    14581 gaaattggcg atatgccgct ggatgtgcag acgcgtttgc tgcgcgtgct ggcagacggt
    14641 cagttttacc gcgttggcgg ctatgcgccg gtgaaagtgg atgtgcggat tatcgctgcc
    14701 actcaccaga atctcgaaca gcgggtgcag gaaggtaagt tccgtgagga tctgttccac
    14761 cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgtg agcgtcggga agatattccc
    14821 cgcctggcgc gccatttttt acaggttgcc gcgcgagaac tgggcgtaga agcgaagttg
    14881 ctgcatccgg aaaccgaagc cgcgttgacg cgtctggcgt ggccaggcaa cgtgcgccag
    14941 ctggaaaaca cctgccgctg gctaacggta atggctgccg gacaggaagt gttgattcag
    15001 gatttgcctg gcgaactgtt tgaatcaacg gttgcggaga gtacttcgca aatgcaaccg
    15061 gacagttggg cgacgcttct tgcgcagtgg gcagacagag cgctacgttc cggtcatcaa
    15121 aatctgcttt ccgaagcgca gccagagctg gagcggacgt tactgacgac cgcgttgcga
    15181 catacgcagg ggcataaaca ggaggcggcg cggctactcg gctggggccg caacaccctg
    15241 acgcgtaagt taaaagagct ggggatggag tgattcacgg cttgtgtgta aagattgatt
    15301 attgagcgca aattgctggt attttgcgct ttactgttcc gataagttca gtatgatctt
    15361 gcccggaaaa cggggagagt cattatgctg gaatcaataa ttaatctggt atcgagtggc
    15421 gcagttgaca gccacacacc gcaaactgct gttgctgccg tgctgtgtgc cgcgatgatt
    15481 gggctgttta gttgagtttc agattgctga tgaagtgctc tttgttcatg ccagatgctg
    15541 cgtgaacgcc ttatccggcc tacaaaagca atattttcaa caggttttgt aggcctgata
    15601 agcgtagcgc atcaggcaat cttgttctca gcacattgat ggcgctttcg ttttctactt
    15661 tgtaaacgaa gcgccattca ctatttaaat cacccgagag aactgctgca tccgcgcttt
    15721 ctggcgcaga taggtatcaa agcacatgca aatgttgcgg atcagcaagc gacctttcgc
    15781 cgtcacctgt attcccttct catccacatc caccagccca tcttttgcta acggggcgag
    15841 cagcttgaga tcttccgcaa agtaatcagc gaagtgcaaa tcccactgtt tctcaatagg
    15901 ggcgtaatcc agacggaagt tgcagatgag cgacttaatc acatcgcggc gaatacagtc
    15961 atcacgcgtt agcgcaatac cacgccacag cgcattgcct tgttcatcca cttgctgata
    16021 gtactgcttc aactctttct ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga
    16081 aacgcccatc cccagcagat cggtatcgcc ctgagtggtg tagccctgga aattacgatg
    16141 cagcacgcct tcacgctggg ccaccgccag ctcgtcatcc ggacgggcaa agtgatccat
    16201 accgataaac tgatagcccg attgcgtcag gaaggcgatg gtttcctgca ggatatcgag
    16261 tttttgctgc ggactcggca ggtcagcatc tttgattttg cgctgagcag caaaaatggt
    16321 cggcagatgc gcatagttaa agacgctcag acggtcaggg tttagctccg ccacgcgttt
    16381 cagggtaaag gcgaaacttt ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt
    16441 ggtggaggta aagccaatct cacgcgcatg gttaagcagc gcaaagataa actcttcatc
    16501 ctgctcgcgg ttaaccagac gttgcacttc tttgttgaag tcctgcacgc ccatactcag
    16561 gcgattaaag ccctcggcgc gtaaatgatc gagtacatcc agttcgattt cccgcggatc
    16621 gacttcgatc gaaatctccg catcggcatt gaactggaag ttttcgcgca gcagcttcat
    16681 caggcggctg atttgcgctt tattaagata cgtcggcgtt ccgccgcccc agtgcagctg
    16741 gctgacgtga cgcccggcga acagcggtgc acgatggacg atttcttgct ccagcgcgtc
    16801 cagatactga tcggccttgt gctgctggcg agtaacaatc ttattgcagc cgcagaagta
    16861 acaaagctta tggcagaacg ggatatgtac gtagagagat aatggacgct caggatagcg
    16921 cgccacggct tgtaaaaacg cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg
    16981 gtacgaggtg tatcgtggcc cggaatagtt atatttctgg atcagggcca gatcccagtc
    17041 gatttgctgt acagacatgc tcactccttc cgatggcgtc tctggcggct acggcgtacc
    17101 ggcggatgcc cgtttcgggt catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac
    17161 cgctgtagtt taacgaataa ccacaccaga taacatataa ccggaagggt tatcagcaga
    17221 acgggaaggc ccacggcggc aatccgttag ttgccccgca gcagacgcat catgtcttct
    17281 tgcttctcgt cttcttcctc ttcttcgtca tcgtcataag agaggccgag tttctgcatc
    17341 aactcatcaa tacgatccag tttagcatcc acccaggatt gctcttcggc actcagggtt
    17401 tcgcctgctt ccagacgttc cagcagcgca tccagacgct catccgtttc tagtaattcc
    17461 aactccgcct gcggtgaaag cataggtttc tcactcttcg gtttgtgctg tttggtgact
    17521 ttttcagtca cgcccaatgg aataggggtt ttactgccaa tacgtggatc tttcggtgcg
    17581 ttctggcctt tgctgcctga cgtgttgttt ccgcccgctg cgcggctgcc cggcgcatga
    17641 ccacgacgtt ttttctgacg cttgcggtca cgagcttcct gattcagctc ttcgcgcgtt
    17701 ttgcgacgcg cttttgcatg acctttgctg cgtgatggtg aagatggttt cataatggtt
    17761 cgtcttttag ccgttttatt cagtatagat ttgcggcgga atctaacaga aagcaagcaa
    17821 agaaaaaagg cgacagatta ctctgtcgcc ttttttcctg actcataacc cttaacgggt
    17881 cttacaatcc ttgcaggccg ccaacacacc ctgtttttcc attagccgga acaatccgtg
    17941 ttgattccat ttccgtttaa ttacgtctcc ggacgtttgt cttcctgaca atcctctgtc
    18001 ttcgcctcct ggcgctcctg accctcatcc tgagtcattg ttcctgttag cgtcctcgct
    18061 ctatgggtgc tactttacgc ctttgcttta aacaaacaat caaccgccat cagtatttgc
    18121 acattttcag agttttatat aaaagcaatt aactgattta tatagcttat ttgacatgca
    18181 tcgcagaatt ttctctgcta tttcgcataa agactatcgg caatgtctca caaagcgtat
    18241 ggcttttact tacaacgctc acgccgaaaa atccgccttt tattatggtt caggtataat
    18301 cgccgcaaag ctttgactga cggagacgac cgctttgact aatttgaatt atcaacagac
    18361 gcattttgtg atgagtgcgc ctgatattcg ccacctacct tccgataccg gaattgaagt
    18421 ggcttttgca ggccgttcca acgcaggtaa atccagcgcg ctgaacacgc tgactaacca
    18481 gaaaagcctg gctcgtacct caaaaacccc tggacgcacc cagcttatca acctgtttga
    18541 agtggctgac ggcaagcgtc tggttgactt gcctgggtac ggttatgcgg aagtcccgga
    18601 agagatgaag cgcaaatggc agcgcgcgct cggcgagtac ctcgaaaaac gtcagagcct
    18661 acaaggtctg gtagtgctaa tggatattcg ccatccgctg aaagatttgg atcagcagat
    18721 gattgagtgg gcggtagaca gcaatatcgc cgtgctggta ctgctgacca aagcggacaa
    18781 actggcaagc ggcgcacgta aagcgcaatt gaatatggtg cgtgaagcgg tgctggcgtt
    18841 taacggtgat gtgcaggttg aaacgttttc ttcgctgaag aaacaaggcg tggacaagct
    18901 gcggcagaaa ctggatacct ggtttaacga gatgcagcct gtagaagaaa cgcaggacgg
    18961 cgaataattt tcttacctta atgcttatgc cggatgtggc gtatccggcc cataaattca
    19021 atagattaca attctaccgt tggcctgata aacatggtgc atcaggcatt acggatcttt
    19081 cctttcgccc aataaaaaac gccccagtca ttactgactg gggcggctaa aatattcagc
    19141 caaatccgat tacgtgaagt aaaaggtctg aaagatagaa catcttacct ctgtacccta
    19201 cgcgactaac tttactcttt tttgttcagt tcagaaagca ctttttgtaa ttttttttca
    19261 gttgttgcat aggaatttct aatagccatc acaaaacgtg acagcttatg ttgcttactt
    19321 acgaaaaaag ggcatgttca ggcgaatctt agtgcgcctg atcccagttt tcgccactcc
    19381 ccacttccac cagcaacggc acatccagac gggtacagtt ttccatcagt tgatgaatct
    19441 gcttcgcaac ggcatcgaca tcatctctat gaacttcaaa taccagttca tcgtgtacct
    19501 gcatgatcat acgtacacgc ggttgctcag cctgtaacca cgcatcaacg gcaatcatcg
    19561 cccgtttgat aatgtcggcg gcggttccct gcattggcgc gttaatggct gcacgttcag
    19621 ccgctgcacg acgagcacca ttgctggatt tgatatccgg cagatacaga cggcgtccgt
    19681 ccagcgtttc aacgtagccc tgctctttcg cctgagcacg ggtgcgttcc atatactcca
    19741 gcacgccagg gtagcgttcg aagtaaaggt ccatgtactt ctgcgcttct ttacgtggaa
    19801 tgttcaattg ccgcgccaga ccgaaagcac tcatgccata aatcagacca aagttgatcg
    19861 ctttcgcgct acggcgttgc tcgctggtga cggtttccag tggcaaacca aacacttctg
    19921 ccgccgttgc ccggtggata tcttttcctt ccgcgaatgc ggtcagcaag cctttgtcac
    19981 gcgagagatg cgccataatg cgcagttcaa tctgcgagta gtccgctgag acaatcacat
    20041 aatcctctgg cgcaataaac gcctggcgga tacgacgacc ttcttcgtta cgcaccggaa
    20101 tgttttgcag gttaggatcg gttgacgata aacgtcccgt tgcagttaca gcctggtgat
    20161 aagaggtatg cacacgcccg gttttcgggt tgatcatcag cggcagcttg tcggtgtaag
    20221 tcgatttcag cttcgccaga ccacgatact ccagaatcac ttttggcaac ggatagtcca
    20281 gcgccagttc ttccagtacc tcttccgacg ttgacggcgc gccacccggc gttttcttca
    20341 gcggtttaat gccctgtttt tcaaacagaa tggtttgtaa ctgcttggtg gaagaaaggt
    20401 taaattcctc acctgcaatt tcatgcgctt tcttttccag ctcagccaga cgaagggtga
    20461 gctcttcaga atgattgtgc agcactttcg gatcgatctt cacaccgtta cgttcaatgc
    20521 gtgaaagtac tggcaccagc ggcatttcga tattctcgaa gacgttcaac ggccctttgt
    20581 gtttttgcag atccggccac attttcagat gcaactgcaa ggtgacatca gcatcttcgg
    20641 cggcgtaacg tccggcctct tcgagggcaa tctggttaaa agtcagttga tttttgcctt
    20701 tacccgcaat ctcttcaaaa gtgatggttt tgtgcttcaa ccaacgttcc gcgaggctgt
    20761 ccatatcgtg acgcccggca acgctattga gaatgtagga ctccagcatg gtatcaaacg
    20821 caatcccacg cagctcaatg ccgtaattcg ccagaatacc gcgatcgtat ttcaggtttt
    20881 gcccgacctt cagcgccttt tcatcttcca gcagcggttt tagcaactcg agtgcacgct
    20941 cgcgagagat ttgatcgggc gcatcaagat aatcatgagc aaccggaata tatgccgcta
    21001 cgcctggctc gatagcaaaa gaaagcccga ccaggttagc tgagatgtta tcaaggctgt
    21061 cggtttcggt atcaaatgca aataccggcg ctttttccag cttcgcaatc cacgctttca
    21121 gtgtttcttc atcaaggatg gtgacgtagt tgtcataaga aatcaccgtt gccgtcactt
    21181 ctggtgcttc gtctgcaaca ctggtttcct gtggcttcgc ggctggtttt gcccctttgg
    21241 cctgtaacca tttgcccgct tcgacatcag cagtccagcg tttgaactca tactttttga
    21301 acagccccaa caactcttcc gctgccggtt gctgcacttc cagttgttca caggtcagct
    21361 ccagttcaac gtcggtttta atcgtcgcca gctggtatga gagataagca acttctttgt
    21421 tttgctcgag cttcgctgcc attgttttcg cgccacggaa gctcaaccca gcaatttttt
    21481 ctggctcggc atacagcgta tccagcccgc caagaccttg cagcaatgcc tgcgcggttt
    21541 tttcaccgac gcccggtacg ccaggaatgt tatcagagga gtcacccatc agcgccagga
    21601 aatcgatgat cagttctggc ggcacgccgt acttattcac cacctcttcc ggtccgagga
    21661 tggtattcgt catggtgttg ataagcgtaa tatttggcgt caccagctgc gccatatctt
    21721 tatcgccagt gctgatcagc accggacgcc cggctttttc ggcttcgcgc gccagagtac
    21781 cgataacgtc gtccgcttct acgccagaaa ccgccagcag cggcagtccc atcgctttaa
    21841 ccatcgcgtg caagggttcg atttgtgctc gcagatcgtc cggcattggc gggcgatgtg
    21901 atttgtaatg ttcaaacagt tcatcacgaa aggtttttcc cttggcgtca aagaccaccg
    21961 ctgcatgcgt cggtttatat tgcatgatca gactgcgcag catgttgagg acaccataca
    22021 tcgcaccggt cggctcgcct gcgctgttag tcagcggggg aaacgcgtga tatgcgcgat
    22081 aaagataaga tgaaccatct acaaggataa gtggattttg ggggatctga accataatgt
    22141 ccgtgcctgt ttatcagatt atgggtaaag gatgccacag acaggataaa aacatgagtt
    22201 tttcaccgct tttcgtgaaa atcttttgta gatcttctgg atcgctcgca aatcgtcatg
    22261 tggataactt tgtgcataaa ttatttacat cgcttttaat ttattatcat gctttttaca
    22321 gcatgatgtt tgtctttgtt tttcatgcag ttaaaaataa atccatccct aataattgag
    22381 ataatgggat tttttctaat gtggatagat atgaattatt tttctcctta aggatcatcc
    22441 gttatttggg tcgttttctg ctaaaatctg cgccttgcgc ttctttagcg cactgtctat
    22501 ggctaacctt ttgaataaat tcattatgac gagaatactc gctgcgataa cccttttgct
    22561 gagtatcgta ttgaccattt tggtcactat tttttgttcc gtcccgatca tcattgccgg
    22621 gattgtaaaa ctattgctac ctgtgccagt catttggcga aaggtttcgc gtttttgtga
    22681 ttttatgatg tattgctggt gtgaaggtct ggcggtatta ctgcatctga acccacacct
    22741 gcaatgggaa gttcacgggc tggaagggtt aagtaagaag aactggtatc tgcttatttg
    22801 taaccaccgt agttgggcag atattgtcgt actgtgcgtg ctgtttcgta agcacattcc
    22861 gatgaataaa tatttcctca agcaacagct ggcctgggtg ccatttcttg gcctggcgtg
    22921 ctgggcgctg gatatgccgt ttatgaagcg ttattcccgc gcttatttat tacgtcatcc
    22981 ggagcgacgt ggtaaagatg ttgaaaccac tcggcgttct tgtgaaaagt ttcgcctgca
    23041 tcccaccacc attgtcaatt tcgttgaagg ctcccgtttc acgcaagaaa aacatcagca
    23101 aacccactcc actttccaaa acttgttgcc accaaaggcg gcaggcattg cgatggcgct
    23161 aaatgtactg ggtaaacaat tcgataaact gcttaatgtt acgttgtgtt atccggacaa
    23221 taaccgtcag ccgttcttcg atatgttaag cggtaaactg acgcggattg tcgtacatgt
    23281 ggatttacag cctattgccg atgagttaca tggcgactac atcaatgaca aaagctttaa
    23341 gcgccatttt cagcaatggc tgaactcatt gtggcaggaa aaagaccgac ttctgacgtc
    23401 gttgatgtca tcacagcggc aggacaagta aattttgctc gcctctgaat gagcagaggc
    23461 gagtgagtat tttaatgaac aaaacgtccg gcacgagaca taaattcttc ttcgctcatc
    23521 tcctgtccgt taaaaacaac tttacccggt gtataacgca gctgcagcga ggcggtattg
    23581 ttgtcaatcg tgattaactg aaacatctga cctaatgtca tcatcccgct gatttgttta
    23641 tcagcctgct tttgcgcttt ttccgcatcc attccttcag ataaattaag ctgttttgcg
    23701 gtttctgtga cgacattaag cggtaacttc acatcaaaat tgagcgattt gatatctttg
    23761 tttgtggatg atgaagattt ggctgggtcg gcaatactga tatccagatt ggcatttagt
    23821 tcgccgagtg cgttcttcca tcttaccggt tgtttaatgg tcggctcact ttgttgtaat
    23881 aacggtaaat actctttgaa caatgcagca ttcacttctt gcagagcaac ttcatcgttt
    23941 gctaattcag ggtgtgcagc aagctgcttc tgcatcgcaa tgttatactg gatgataaat
    24001 tggcgcaccg ctgagggatc aatagattca gcaatcaatg accactcacc actaccgagt
    24061 tgctgattcc cttttgttaa cttatcaagt tcgtaggtca gattgacatt gatataatct
    24121 ttgtctttaa cgcgatccag cctggttttt gcaacgaaag catcgatttg ggcaacgtct
    24181 tccccgtgat tgataatgtt aatctgatta aatttcgact cgctttcacc aaccggaaat
    24241 ttcttttctt ctaatcgcgc cagagaatca atggtaaaac tttttgccgt tactttgttc
    24301 attggtgata attgcagaac aatatcttcg actttgcctt caatatccag gttagacaat
    24361 gaactactat caccattaaa agtaaactgg ccctcaccaa aagaaaactt tccatactct
    24421 gcaggggcaa catctaatgt cgtagagaat gagccaccaa acgcgaatcg caatgtcgct
    24481 tccacgggcg atttattttt cgcggcgata aatagcggtt gtgtcagttc attattcact
    24541 aagttcactt ttgccgctgc cagtgctggg atgacattac catgcattaa cattgtgatg
    24601 ggcaacggac cgtgctcaac atccacatca aatacaactt tctggcctgg cttgatattg
    24661 agatcgggtg caccgttatc gaatgtcatt tgcatttgaa aacgggaatt aaaaaagcct
    24721 ttgtcaaaat tttgataact taacgtcata tcgtaggcat gttcaccttt ctttttagca
    24781 tcgttaaaat ctttaataaa tttttcgaca ccaggctgaa tttgcgtacc tgtgtaccat
    24841 gtgccaccac cccagattac ggctaacgca acaataacac ctgtagctga cttacgtatc
    24901 atataaatat tccctgataa aacagcaaaa ctttgaatat ccacttatgc tgaaataatg
    24961 tccagcggca ggatgcatta tcagaatgta tttatattta ttaaataata aaaaaagccc
    25021 gtgaatattc acgggcttta tgtaatttac attgaattat tttttctcgg acagatattt
    25081 cactgtatca gcatactgct gaacaaaaac atccatattg ctggtatcca taccctgcgg
    25141 attcagctga tatttaccgt taacaaacat cgccggaaca ccacgcaatt gcacgtcagc
    25201 tgcagctttt tcctgctgag cgaccagaga tttcaccacg aagctgttcc acgccgcgtc
    25261 gtactcttca cctttaatac ctgtgttgat aaatacatcg cggatatcag atgctgaacg
    25321 aatggtctgg gttttctgta cgccttcaaa cagcggaaca gtcactttgt cttccacgcc
    25381 cagcgccatc gccacagccc atgcctgagt cagatctttg cccaggtcac cacccatgaa
    25441 gttgacgtgg tatttagtca tcttcacgcc ttccggcagt tttttcttca cattatcaga
    25501 aatatgcaga acttcttcaa actgatagca gtgcgggcag aagaaagaga aaaactccag
    25561 cacttgcggc gcgccagcta ccggtttttc cagggtagtg tactgtttac catcttcata
    25621 ctgcgccgcc gatgcgctaa acgctaaaac taaaccagcc agcgccagcc aaatcttttt
    25681 catgatctac tctctccgat taatacatag gtgttaattg caaagggggt tcttgtagaa
    25741 cttttgcctg ttctataaaa gtcgccgtct gtcgtagcca gtaatcttcc ccggttaacc
    25801 acgggaaatt tttcgggaac gcgggatcag cccaacgccg cattagccag gcaagataat
    25861 aaaccaaacg catggcgcgt aaaggttcaa tcagtccgat ttcagcggtg tcgaactcgc
    25921 taaattcttc ataagcttca ataatagttt ccagttgcat ccgctgctcg gctttatcgc
    25981 cattgagcaa catccacaaa tcctgaatag ctggaccatt acgtgcatca tccagatcaa
    26041 caaacattgg accatcgcgc cagagaatat tcccggcgtg gcagtctcca tgtagccgca
    26101 gaacggtgaa atcttcccgc cagtgtgctg taacggcggc gatcagctca tctgtcgctt
    26161 tcaggaatgc cgctttcaac ccggaaggta tcagtgtagc gtcctcaaac agcttgcgtg
    26221 gctcaatgag atattcattc agaccgatgg tcgggcgatg gataaaaagc tgtttgcgcc
    26281 ccgtctggtg catacgccct aaataacgcc caaccgcttc catctgatcg atattatcag
    26341 cttcgaactg gcgaccaccg acgcttggaa aaacagcgaa ataaaatccc tgatgattca
    26401 ataaagtctg accgttaaaa gccacaggtg ctgcaaccgg aacttcatca tttaccagct
    26461 gcaacgcaaa ttgatgttct tcgaggattt gatcggctgt ccaacgttca ggacgataaa
    26521 atttgacgac aaaacgtcga cgatcttcgt cctgaaattg atagacacgg ttttcatagc
    26581 tgttaagcgg ggtaagaccg gaatccaccc ggatcccttg ctcaaacaga gcgtccatga
    26641 tggtatccgg gtgtagtgtc tggaaagtaa aagcgctgtt gttcatccca tcatccggaa
    26701 aacattacga atgatgaagg atatcatttc acgatgattt tggtggcgcc gcttacaagc
    26761 ttttactctt taattacgcc gcgagcgcgt aacagcgccg ttttgaagtc ttcttcataa
    26821 tctttctgca aacccgggat caccgcatct ttcgcggaat cacgcatttt caggtgatag
    26881 atcagaatgt catccgtcaa atccgccagt tcgccgtcaa aacctgactc tttcgccagt
    26941 ttctgcaaaa attgcaacag gttaaggtct ggctcttttt gccaggctgg ctggaggagt
    27001 tcaataactt cattcagacg tttacatttc atagtaatgc tccttactct tgagacagac
    27061 acgttagcag ggtcaatccc acaataaaag aggcgatatc ggtgaatctg atgacgacga
    27121 taacaggcgt tgtgctggca ggcggtaaag ccagacgaat gggcggcgta gataaaggat
    27181 tgctggactt aaatggcaaa ccgctgtggc aacatgtcgc tgacgcgctt atgacgcagc
    27241 tctctcacgt cgtggttaat gctaatcgtc atcaggaaat ctatcaggca ggcggtctga
    27301 aagtgattga agattcactg gcggattacc caggccctct ggcagggatg ctttcagtaa
    27361 tgcagcagga agctggtgag tggtttttgt tttgtccgtg cgatacgcct tacattcccc
    27421 atgatttagc cgcccggctt aatcatcagc gcaaagatgc gcctgtcgtg tgggtccatg
    27481 acggtgaacg cgatcacccg actattgcta tggtaaaccg cgctattgag cctttattac
    27541 tagaatatct gcaagcagga gaacgccggg taatggcatt tatgcgtctg gctggcggtc
    27601 atgcggttga tttcagcgat cataaagatg catttgttaa cgtgaatacg ccagaggagc
    27661 ttgcccgatg gcaggaaaaa cgatgatacc gttactcgcc tttgccgcgt ggagtggtac
    27721 cggaaaaact acgcttttga aaaaactgat cccggcatta tgcgccagag ggatccgtcc
    27781 agggctgatt aagcatacgc accatgatat ggatgttgat aagccaggca aagatagcta
    27841 tgagctgcgc aaggctggcg cggcacaaac catcgttgcc agccagcaac gatgggcctt
    27901 gatgacggaa acaccagacg aagaagagct ggatctacag tttctcgcaa gtcgaatgga
    27961 tacctcaaag ctggatttga ttctggtcga agggtttaag catgaagaga tcgcaaagat
    28021 tgtgctgttt cgcgatggag ccggacatcg acctgaagaa ttagtgatag acaggcatgt
    28081 tattgctgta gccagtgatg tgccgcttaa tcttgatgtc gcattactgg atattaatga
    28141 tgttgagggg ctggctgatt ttgtggtgga gtggatgcaa aatcaggacg gataagcaag
    28201 agtaggctgg agca
//
LOCUS       DAZFBR010000062        27914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFBR010000062 DAZFBR010000000
VERSION     DAZFBR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.062"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1443
                     /gene="citT"
                     /locus_tag="ACHJZ2_002827"
     CDS             <1..1443
                     /gene="citT"
                     /locus_tag="ACHJZ2_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO4568904.1"
                     /translation="IWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILE
                     PIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFG
                     AFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTV
                     FPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSK
                     IAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGAL
                     TRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNT
                     LVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLS
                     AHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKS
                     KDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            1557..2363
                     /gene="rna"
                     /locus_tag="ACHJZ2_002828"
     CDS             1557..2363
                     /gene="rna"
                     /locus_tag="ACHJZ2_002828"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO4568905.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            2593..3003
                     /gene="rnk"
                     /locus_tag="ACHJZ2_002829"
     CDS             2593..3003
                     /gene="rnk"
                     /locus_tag="ACHJZ2_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO4568906.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            3141..3230
                     /gene="yldA"
                     /locus_tag="ACHJZ2_002830"
     CDS             3141..3230
                     /gene="yldA"
                     /locus_tag="ACHJZ2_002830"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO4568907.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(3234..4472)
                     /gene="ybdR"
                     /locus_tag="ACHJZ2_002831"
     CDS             complement(3234..4472)
                     /gene="ybdR"
                     /locus_tag="ACHJZ2_002831"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO4568908.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            4693..5121
                     /gene="uspG"
                     /locus_tag="ACHJZ2_002832"
     CDS             4693..5121
                     /gene="uspG"
                     /locus_tag="ACHJZ2_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO4568909.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(5242..6807)
                     /gene="ahpF"
                     /locus_tag="ACHJZ2_002833"
     CDS             complement(5242..6807)
                     /gene="ahpF"
                     /locus_tag="ACHJZ2_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO4568910.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(6936..7499)
                     /gene="ahpC"
                     /locus_tag="ACHJZ2_002834"
     CDS             complement(6936..7499)
                     /gene="ahpC"
                     /locus_tag="ACHJZ2_002834"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO4568911.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            7871..8617
                     /gene="dsbG"
                     /locus_tag="ACHJZ2_002835"
     CDS             7871..8617
                     /gene="dsbG"
                     /locus_tag="ACHJZ2_002835"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO4568912.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            8826..9728
                     /gene="ybdO"
                     /locus_tag="ACHJZ2_002836"
     CDS             8826..9728
                     /gene="ybdO"
                     /locus_tag="ACHJZ2_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4568913.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNECF"
     gene            9875..11095
                     /gene="ybdN"
                     /locus_tag="ACHJZ2_002837"
     CDS             9875..11095
                     /gene="ybdN"
                     /locus_tag="ACHJZ2_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4568914.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            11068..11697
                     /gene="ybdM"
                     /locus_tag="ACHJZ2_002838"
     CDS             11068..11697
                     /gene="ybdM"
                     /locus_tag="ACHJZ2_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO4568915.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(11698..12858)
                     /gene="ybdL"
                     /locus_tag="ACHJZ2_002839"
     CDS             complement(11698..12858)
                     /gene="ybdL"
                     /locus_tag="ACHJZ2_002839"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO4568916.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            12967..14055
                     /gene="hcxA"
                     /locus_tag="ACHJZ2_002840"
     CDS             12967..14055
                     /gene="hcxA"
                     /locus_tag="ACHJZ2_002840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO4568917.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(14065..14262)
                     /gene="ybdD"
                     /locus_tag="ACHJZ2_002841"
     CDS             complement(14065..14262)
                     /gene="ybdD"
                     /locus_tag="ACHJZ2_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO4568918.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(14444..16549)
                     /gene="cstA"
                     /locus_tag="ACHJZ2_002842"
     CDS             complement(14444..16549)
                     /gene="cstA"
                     /locus_tag="ACHJZ2_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO4568919.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(16730..17143)
                     /gene="entH"
                     /locus_tag="ACHJZ2_002843"
     CDS             complement(16730..17143)
                     /gene="entH"
                     /locus_tag="ACHJZ2_002843"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO4568920.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(17146..17892)
                     /gene="entA"
                     /locus_tag="ACHJZ2_002844"
     CDS             complement(17146..17892)
                     /gene="entA"
                     /locus_tag="ACHJZ2_002844"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO4568921.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(17892..18749)
                     /gene="entB"
                     /locus_tag="ACHJZ2_002845"
     CDS             complement(17892..18749)
                     /gene="entB"
                     /locus_tag="ACHJZ2_002845"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO4568922.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(18763..20373)
                     /gene="entE"
                     /locus_tag="ACHJZ2_002846"
     CDS             complement(18763..20373)
                     /gene="entE"
                     /locus_tag="ACHJZ2_002846"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO4568923.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(20383..21558)
                     /gene="entC"
                     /locus_tag="ACHJZ2_002847"
     CDS             complement(20383..21558)
                     /gene="entC"
                     /locus_tag="ACHJZ2_002847"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO4568924.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            21747..22703
                     /gene="fepB"
                     /locus_tag="ACHJZ2_002848"
     CDS             21747..22703
                     /gene="fepB"
                     /locus_tag="ACHJZ2_002848"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4568925.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(22707..23957)
                     /gene="entS"
                     /locus_tag="ACHJZ2_002849"
     CDS             complement(22707..23957)
                     /gene="entS"
                     /locus_tag="ACHJZ2_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO4568926.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            24068..25072
                     /gene="fepD"
                     /locus_tag="ACHJZ2_002850"
     CDS             24068..25072
                     /gene="fepD"
                     /locus_tag="ACHJZ2_002850"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO4568927.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            25069..26061
                     /gene="fepG"
                     /locus_tag="ACHJZ2_002851"
     CDS             25069..26061
                     /gene="fepG"
                     /locus_tag="ACHJZ2_002851"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO4568928.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            26058..26873
                     /gene="fepC"
                     /locus_tag="ACHJZ2_002852"
     CDS             26058..26873
                     /gene="fepC"
                     /locus_tag="ACHJZ2_002852"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO4568929.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(26870..>27914)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJZ2_002853"
     CDS             complement(26870..>27914)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJZ2_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO4568930.1"
                     /translation="NLISVLWRAKKTVMAVVFAFACAGLLISFILPQKWTSAAVVTPP
                     EPVQWQELEKTFTKLRVLDLDIKIDRTEAFNLFIKKFQSVSLLEEYLRSSPYVMDQLK
                     EAKIDELDLHRAIVALSEKMKAVDDNASKKKDEPSLYTSWTLSFTAPTSEEAQTVLSG
                     YIDYISALVVKESIENVRNKLEIKTQFEKEKLAQDRIKMKNQLDANIQRLNYSLDIAN
                     AAGIKKPVYSNGQAVKDDPDFSISLGADGIERKLEIEKAVTDVAELNGELRNRQYLVE
                     QLTKANINDVNFTPFKYQLSPSLPVKKDGPGKAIIVILSALIGGMVACGSVLLRYAMA
                     SRKQDAMMADHLV"
BASE COUNT         6718 a         7593 c         6971 g         6632 t
ORIGIN      
        1 atatggaaac tattggcccc actggtggtg atgggtgtca tgtttcttat ccctgtcccc
       61 gacggtatgc cgccgcaggc atggcattac ttcgctgtgt ttgtggcaat gattgtcggc
      121 atgatcctcg agccaattcc ggcaacagcg atcagtttta ttgcggttac tatttgcgtt
      181 attggcagta attacctgct ctttgatgcc aaagaattag ctgacccagc gtttaatgcg
      241 caaaaacagg cgctgaaatg gggcctggct ggtttttcca gcactacggt atggctggta
      301 tttggcgcat ttatttttgc attagggtat gaagtttccg ggttaggtcg tcgcattgcc
      361 cttttcctgg tgaaattcat gggcaaacgc acgctgacgt tgggttatgc gattgtcatt
      421 atcgacattc tgctggcacc gtttacaccg tccaacaccg cgcgtaccgg gggtacggtt
      481 tttccggtca ttaaaaacct gccgccgttg tttaaatcat tcccgaacga tccgtccgcg
      541 cgtcgtattg gcggctattt gatgtggatg atggtcatta gtaccagtct gagttcgtcc
      601 atgtttgtca ccggtgcggc accaaacgtg ctgggtctgg agttcgtcag caaaattgcc
      661 ggtatccaga ttagctggtt gcagtggttc ctctgcttcc tgccggttgg ggttatcttg
      721 cttatcattg cgccgtggct ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa
      781 gaagtggcaa cctgggcggg tgatgaacta aaaaccatgg gtgcgctgac acgcagagag
      841 tggacgctga ttggccttgt attgctcagc ttaggtttgt gggtatttgg cagtgaagtc
      901 attaatgcta ctgcggttgg tctgctggca gtttcgctaa tgctggctct gcacgttgtg
      961 ccgtggaaag acattacccg ctataacagc gcatggaaca cgctggtcaa cctggcaact
     1021 ctggttgtga tggctaacgg cctgactcgt tctggtttta ttgactggtt cgccggtacc
     1081 atgagtacgc acctggaagg attctcacca aacgcaacgg tgattgtact ggttctggtg
     1141 ttctactttg cacactacct gtttgccagc ctgtctgcgc acaccgcaac catgctgccg
     1201 gttattctgg ccgtcggtaa aggtattccg ggcgtaccaa tggaacaact gtgtatcctg
     1261 ctggtgctgt ctatcggtat catgggctgt ctgacgccgt atgcaaccgg tcctggggtg
     1321 attatttacg gctgtggcta tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc
     1381 ggggtgattt acatctctat gttgctgttg gttggctggc cgattctcgc catgtggaac
     1441 taatacgttc gccaaacgct gaataaatca tcacgcccgc caggttagcc cacctggcgg
     1501 gcttttttat gatttaatag atagtattca gtgtatgtat gagttccaca cccattatga
     1561 aagcattctg gcgtaacgcc gcgttgctcg cggtttctct gcttcccttc tcttctgcca
     1621 acgccgtagc gttgcaggca aaacagtatg gcgattttga tcgctatgtc ctggccctct
     1681 cctggcaaac cggattttgc cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat
     1741 gtcgcctgca aaccgaaacg accaacaaag ctgattttct gaccgtacat ggtctgtggc
     1801 caggattgcc taaatcggtt gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg
     1861 gttgcgctac tcgcccaatc ccgaatctac cagaagcgcg cgccagccgg atgtgttcat
     1921 cgccggaaac cggattatca ctggaaacgg ccgctaaact aagtgaagtc atgccaggag
     1981 ctggcggacg ttcctgcctg gaacgctacg aatatgccaa acacggtgcc tgctttggtt
     2041 ttgatccgga cgcatacttc ggtacgatgg tacgcctgaa tcaagagata aaagagagcg
     2101 aagccggaaa attccttgcg gataattacg gtaaaacagt gagccgccgt gactttgacg
     2161 ccgcctttgc caaaagctgg ggaaaagaga acgtgaaagc agttaagcta acgtgccagg
     2221 gtaaccctgc gtatttgact gaaattcagg tctcgatcaa agctgacgcc atcaacgctc
     2281 cgctttctgc aaactcattc ttgccacaac cacacccagg taactgtggc aaaacctttg
     2341 tgattgataa agcgggttat taatcctctg atttacaaaa cacgctaatt cgtctttcat
     2401 aaaccatgac gattaagaat tatacataat gcgctgcatt gaggaattcc ctgaatgtga
     2461 cgcaaatcac ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc
     2521 gcctgacggt gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga
     2581 catggagtaa aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc
     2641 gatattctgc tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca
     2701 gagttggatc gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg
     2761 aacagccggg ttaaattccg caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg
     2821 tatccggcaa aaatgaccga tagcaatact cagctttccg ttatggctcc ggtaggtgcc
     2881 gcactgctgg gactgcgcgt tggcgattcc attcactggg aacttccggg cggcgttgca
     2941 acccaccttg aagtgctgga actcgaatac cagccagaag ctgctggcga ctacctgctt
     3001 taatcccatc tgtacaagcg ataactcgct ttgcccagag gatgcactcg catcctcttt
     3061 atgcaaaatc agcaattttt cctggttctc actgttcact tttgctgcaa aattattccg
     3121 tttcgtttgt gggaataacc atggcagaag cattttacat cctgatagga tttctgatta
     3181 tggcggcgat tatcgtcatg gctgtccttt acctcgaaaa ccactcctga cgctcatatt
     3241 gttccccccg gcatcgcatt caccagacct gaaaccgcct tctgcgccgc ctctgcgctt
     3301 tgtgcaccgg gtaccagaat caccttacgg cactcctctt cacgcttttc aaagatctca
     3361 tatccccggg cggcctcttc aaacggcata tagtgggtaa caatttcttc tggtttcagt
     3421 aatcctttct caattaacgg taataattct cccagccatg cgtgaacgtg ggtctgtccc
     3481 attttaaacg tcaacccttt atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca
     3541 gcgtagacgc ccggtacgct aacaatgccg ccacgcctga ccgccgcaat acactgacgc
     3601 aacgctttac cgctgctgcc ctccagtttc aggttagtca gcaccgtttc cgtggtgctg
     3661 cctttcgctt caaaaccgac ggcgtctatt actgcatcca cgccccggtg acctgccgtt
     3721 tgttcaataa ttgactgtgc cggatcgcta tcttcatcga aattaatcgg gatcgcgccg
     3781 tagcggtcag cggcgaaacg caagcggtag ggatgatgat caacaacaaa aatctgttcc
     3841 gcaccgagca accgtgcaca ggcgattgtc aacaatccca caggaccggc accatagaca
     3901 gcaacgcttg aaccttgttg gatctgcgca ttttttgctg cctgccatgc cgttggcaga
     3961 atatcagaaa ggaaaagggc tttatcatct gaaagcaaag gcggtacttt aaacggcccc
     4021 acattccctt tagggacacg gacatactcc gcctgtccac caggaacgcc accatacagg
     4081 tgactataac caaacaatgc cgcgggagct ggtatctgtt ttttattgag cgcagcgcct
     4141 ttacccgcat tggtattttc gcaggcggca tattgctgca gtcgacagaa aaaacagtcg
     4201 ccacaagcaa tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacatcc
     4261 tttccggttt caactacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc
     4321 tggggtattt tgcctcgata aagatggagg tcagagccac agatcgccgt tgccgttata
     4381 cgcagaataa tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg
     4441 tgatgtgggc cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga
     4501 gtcagtcttt aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag
     4561 aattcctggt gttcagtgag aatatattca tcacagaaac agttcgtaag gaaaaagaac
     4621 cagacaaata tcagggataa atattataat catggcgaat gatttgttca tgattaacag
     4681 ggagaaaggg ttatgtataa gacaatcatt atgccagttg atgtatttga aatggaattg
     4741 agcgacaaag ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta
     4801 cttcacgtac tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt
     4861 cgttttgaag agcatctgca acatgaagca gaagaacgtt tgcaaacgat ggtcagccac
     4921 ttcaccatcg atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa
     4981 gtcaatgagt tagcagaaga actgggggct gatgttgtag ttattggttc tcgcaaccca
     5041 tcgatttcga cccatctgtt aggttctaac gcctcgagcg tgatccgcca cgccaatctg
     5101 cctgtactgg tcgttcgcta acagataaaa gtatttatgc cgccctgcct ggaacatccc
     5161 ggcagggctt tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt
     5221 gcaggtgaat cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact
     5281 cagagaggct ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt
     5341 acagtcaccc gctgcgaaca cgcctttcac gttggtttcg catttcgcat caatgataat
     5401 ctcgcccatg cggttacgtt cgactgcgcc ttcgagccag ttggtgttcg gcagcagacc
     5461 aatctgcacg aaaataccgg ccagttcgat gttgtgaata tcgccgctga cacgatctcg
     5521 atattccaga ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag
     5581 aataatgtcg acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat
     5641 ttctggcgca aattccagca gcgttacgtg ctcaacgata cccgccaggt caattgccgc
     5701 ttccacgccg gagttaccgc cgccgataac cgctacgcgt ttacctttaa acagcgggcc
     5761 gtcgcagtgc gggcagtagg tcacgccttt ggtgcgatac tgatcttcgc ccggaacgtt
     5821 catgttgcgc cattttgcac cggtcgccac gataatgctg cgtgctttca gtaccgcgcc
     5881 agaagctgtt tcaatctgat gcagaccacc ttcaactgct gctgggatca gtttgctggc
     5941 gctctggctg tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc
     6001 cagcttctgc ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag
     6061 gatctgacca ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta
     6121 aattgctgcc gctgcacccg ccgggccgga accgacgatt aatacgtcat aagcatcacg
     6181 cttgttcagc tcttctgccg cacgtttttc cgcgcccgta tcaattttgg caacgatttc
     6241 agtcaaagtc atgcggccct gaccaaactc tttcccgttc acgaacactg ccggaacgcc
     6301 catcacgtta cgatcagtga tctcgttctg gaaggtgccg ccgtcaattg cagtgtgctt
     6361 gatgcgcggg ttcaatacgc tcatcaggtt cagcgcctgt accacgtccg ggcagttatg
     6421 gcaagagagc gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc
     6481 cagcagagac tgcgcttctt tcgacggatg accaccggtc cacagtaacg ccagtaccag
     6541 cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt ggcccctggt tagaacctgg
     6601 gttggtgatc aggaaagacg gcttacgcac cggcaagctg ttatcttctt taaaggtgac
     6661 tttgtctgac agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct
     6721 gtcatccagc gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag
     6781 ttgagttttc atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca
     6841 agcggcctct gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt
     6901 gccgcagcac ccgattaatt acgcgattgc ggaacttaga ttttaccaac caggtccaga
     6961 gacggagcca gagttgcttc accttctttc catttagccg ggcaaacttc acctgggtga
     7021 gaagctacgt actgtgctgc tttgatttta cgcagcaggt cagacgcgtc acggccaatg
     7081 ccttcagcgg taacttcgat tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca
     7141 cggtcagcca gaccttcatc ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc
     7201 gggtcgccga tcatcgcata tttgatttta gcgatggttt cagagctgct gtgccatgct
     7261 ttgtgggtga agtgagtatc ggtagatact gcgtatacgt ctacgcccag tttctgcagt
     7321 tcttcgtagt ggtcagcaac gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc
     7381 gggtagaaga agaagacgct ccagcggcct tcggtatctt tttcggtgat ttcgatgaat
     7441 tcgccgtttt tgaatgcctg gtttttaaaa ggtttaattt tggtgttaat caaggacatc
     7501 tatacttcct ccgtgttttc gatgagatgt aaggtaacca atttttaccg attcggctaa
     7561 tgcgtttcca ttatcaaatc gataagtttt accttacaac cttcgtaaga caactttgtg
     7621 aactttgcac cgccagcggc gataaaaagt aaaatggccc cctttccagg tgaccgtgta
     7681 atctgaacta ccctttggta tcttcagcgc cagtctgcag gctgcgttgt tgctcttaca
     7741 aatgcaacaa cgacatggat tacaacaccc tcataaacaa agggcaatca cctgatctaa
     7801 gctcttacct atgacaatga taggttatgc cttttactcg acttttgcac tgactgaaaa
     7861 ggacaaatta atgttaaaaa agatactttt actggctctg cttcctgcaa tcgccttcgc
     7921 agaggaactt cctgctccag taaaagcgat tgaaaaacag ggcattacaa tcatcaaaac
     7981 gttcgatgcc cccggaggaa tgaaaggtta tctcggaaag tatcaggata tgggcgtcac
     8041 catctacctg actccagatg gtaagcacgc tatctctggt tacatgtaca acgagaaagg
     8101 tgaaaacctg agtaacacac ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat
     8161 gtggcaacgg atggaacaat cccactggct cctcgacggt aaaaaagatg cgccggtcat
     8221 tgtctacgtc ttcgccgatc cgttctgccc atattgtaaa cagttctggc agcaggcgcg
     8281 cccgtgggta gattctggca aagtacagtt gagaacattg ttggttgggg ttatcaaacc
     8341 agaaagtccg gcgacagccg cggcaattct tgcctccaaa gatcccgcaa aaacctggca
     8401 agaatatgaa gcctctggtg gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga
     8461 gcaaatgaaa gtgttaagcg acaacgagaa actcatggac gatctggggg caaatgtcac
     8521 tccggctatc tattacatga gtaaggaaaa tacgctgcaa caggccgtgg ggttgcccga
     8581 tcagaaaacg cttaatatca ttatgggaaa taaataaaag gtaattcatg gatggttgaa
     8641 ctatatccct gcttttcaac catcctaaaa aagcatgaat tgctcgctct gccctaagtg
     8701 gctgtgctat tttagaaaaa cttaatcctg agttaagcaa aaaaacataa tcaataaaaa
     8761 tatatacgca aaaaacattg attaagtgaa tatatcatgg aagaaaaata taaccggagt
     8821 agtgtatgac caatctctac gacttgaaaa agttcgactt aaatcttctt gtcatatttg
     8881 agtgtattta ccagcattta agcattagca aagctgctga atcactatat atcaccccat
     8941 cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct ttattcattc
     9001 gctcaggaaa aggtatcgcg ccgacgacca cagggttaaa tctgcatcac catcttgaga
     9061 aaaatctcag aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa
     9121 actttattat tcacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct
     9181 gcctacggca agattcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga
     9241 atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga
     9301 tcagccgttc cgtcatttgc atgccattgc atacgattcg caatactcta atttgtagta
     9361 ataggcatcc acgaattact gacaatagca cttatgaaca aattatggca gaagagttca
     9421 cccaattaat atctaaatca gcaggggttg atgatattca aatggaaata gacgagagat
     9481 ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaatcatt aatagcattg
     9541 cagttacaga tttactggga atagtcccct atgaacttta taactcccac cgtgactttc
     9601 ttaatttaaa agagataaaa ctagaacatc cgttaccttc aataaagctt tacatttcct
     9661 ataataaatc ttctttaaac aatctggtgt tctcacgatt tattgaccgt ctaaatgagt
     9721 gtttttgata tataacctca gacagctact ataactttca cctatttatt cacagcgcga
     9781 atgaaaaata aaacactctg cttttcagtg tttactttat aattaacagt cttatctcac
     9841 tgatcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc
     9901 gctcaatatt ctggaagctg cacgagagcg tattacctgg acactcaaca ccctaccccg
     9961 cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc tgacagccga
    10021 actcgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg aggcgcaatt
    10081 ctcttgcacc attaactatg ttcagtccct gcgcgagttg tacaccgatg tcatcgaaga
    10141 gttttactgg gttgcgctcc cgcttacgac gcaaaattcc ctttcacaat accaacccga
    10201 atggcagtgc tgggaacctg atgtcgaatg ggtacgtcag cccccgcaag atgcgataac
    10261 cgatcctgac tttttctgct tttaccagcc aggcatgacc ttcgaacaat ttgtacgtga
    10321 gtttgccgaa tggttttcac aaaaacgtcc ggcggcgatg atgatcggca tccgtgcgga
    10381 tgagtcctac aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt ttgccgacga
    10441 taaaccctgg actacggccg caccaggcgg tcatagctgg tacatttacc ccatttacga
    10501 ctggaaagtg gctgatatct ggacctggta tgcaaatcat caaagtctct gcaacccact
    10561 gtataaccta atgtatcagg caggcgttcc tctgcgtcat atgcgaattt gcgaaccttt
    10621 tggcccggag caacgacaag gattatggct ctatcacgtt atcgaaccgg atcgctgggc
    10681 tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc atttacgccg gacatgataa
    10741 tcatttctat gggcatcgga aaatcctcaa gccagaacat ttagactggc aagaatatgc
    10801 attattgctg ctcaatagca tgccggaaaa aacagctgag cattaccgca ataaaatcgc
    10861 catttatttg cactggtatc agaaaaaagg catcgaggtt ccacaaaccc agcaggggga
    10921 cattggcgcg aaagatatcc cctcctggcg gcggatatgc aaagttttac tcaataacga
    10981 ttactggtgt cgggcattat catttagtcc tacgaaagcg aagaactatc agcgttataa
    11041 cgaacggata aaaggaaaac gtcaggaatg ggggatacta tgcaacaacg attaacgcag
    11101 gatttaacgc aatttctcgc cagcttgccg gaagatgatc gcatcaacgc gattaatgaa
    11161 atccgcatgg cgatccatca ggttagcccc tttcgtgaag agccagtgga ttgcgttctg
    11221 tgggttaaaa acagccaact tatgcctaat gattacaacc caaacaacgt ggcaccaccc
    11281 gaaaaaaaac tgctgaaaaa atccattgaa atagatggtt ttacccaacc cattgtggtc
    11341 acgcatacgg ataaaaatgc cctggaaatc gtggatggtt ttcaccgcca tgaaattggt
    11401 aaaggctcat catcattaaa attacgctta aaaggttatt tgccggtgac ctgtctggaa
    11461 ggtacgcgta atcaacgcat cgccgcgact attcgtcata atcgcgctcg tggacgccat
    11521 caaatcaccg ccatgtcgga aatcgtccgt gaactcagtc agttgggatg ggacgataat
    11581 aaaattggca aagagctggg aatggacagc gacgaagtgc tgcgcctgaa gcaaattaac
    11641 ggtctgcaag agctgtttgc cgaccgtcaa tattcccgcg cctggactgt caaataacta
    11701 cagctggcgc agtcgttcag ctgctgccag caacgtcgat tccttcttgg caaaacagag
    11761 acgaatcagt ttatgtggga agggatcggc gcaaaacacc gatagcggaa tcgccgccac
    11821 gccgtgctcc cgcgtcagcc actggcaaaa ctcaacatca tccagggtag aaaccgcgct
    11881 gtaatccacc agcaaaaagt atgtgccttc gcacggtaaa atctccagcc gactttcatt
    11941 taaggcattt accagaatat cgcgcttctg gcgataaaag tccggtaacg caagataatg
    12001 ctcaggttct gcacgtagca tatccgcaat tgccagctgt gccggggtat tcaccgaaaa
    12061 ggtcagatac tgatgtacct tgcgaatttc ggcgctgatg ggcgctggcg caacacaata
    12121 acccactttc cagccggtca tatgataggt cttgccaaac gatgacaccg ccaccgcccg
    12181 ctcacgcagc tgcggatgcg ccagcacact ggcatggccc tgttgtgaaa agttgatgtg
    12241 ctcgtagact tcatcgctaa tgacaaaaat ctcgtgcccg gcgatcgcct gccacaaagc
    12301 ggcgaaatca gcctgctgcc agacagttgc actgggatta tgcggagtgt tgaggatcac
    12361 cagtctggtg cgctcgctta acaatgcggc aaattcctgc cagtcaacgc gaaaatgcgg
    12421 tggttgcagt gccatacgct tcactattcc cccagaaagc gcgatggcgg gggcgtaact
    12481 gtcatagctg ggatcaaaac aaatcacttc atcgccattg cgcaccagtg cggtaatcgc
    12541 cgcgtataac gcttccgtcg cccctgccgt tacggtgata tcgctatcgg catctggttg
    12601 atagccatac aaacgttccg ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt
    12661 catgggcgcg tattggtttg ccccctgggc aacgtggtac gccagccgct cctgtaaata
    12721 gcgcggacca tcaaaatcag gaaagccttg cgacaggtta atcgcctggt gttgctgcgc
    12781 cagcgcgctc atctgggtga aaatagtggt gccaagttgt ggaagtttgc tttgtggaat
    12841 cagagggtta tttgtcattt ttattgtacc tgacgaagtt gcgtgttgaa ggcactataa
    12901 cacgatgcta gtatttggca atcaagacgt ttagatgtct aaataaaaca ataaggacaa
    12961 cacaacatgc ctcacaatcc tatccgcgtg gtcgtcggcc cggctaacta cttttcacat
    13021 ccaggaagtt tcaatcacct gcacgatttt ttcactgatg aacaactttc tcgcgcggtg
    13081 tggatctacg gcgtacgcgc cattgctgcg gcgcaaacca aacttccgcc agcgtttgga
    13141 cttccagggg caaagcatat tttgtttcgt ggtcattgca gcgaaagcga tgtacagcaa
    13201 ctggcggctg agtccggtga cgatcgcagc gtggtgattg gcgtcggcgg cggcgcacta
    13261 ctcgacaccg cgaaagccct cgcccgccgt ctcggtctgc catttgttgc cgctccaacg
    13321 atcgccgcca cctgcgccgc ctggacaccg ctctccgtct ggtataacga tgccggacag
    13381 gcgctgcatt atgagatttt cgacgacgcc aattttatgg tgctggtgga accggagatt
    13441 atcctcaatg cgccgcaaca atatctgctg gcggggatcg gtgatacgct ggcgaaatgg
    13501 tatgaagcgg tggtgctggc tccgcaacca gaaacgttgc cgctaaccgt gcgactgggg
    13561 atcaataatg ctcaggccat tcgcgacgtc ttgttaaaca gtagcgaaca ggcgcttgcc
    13621 gatcagcaaa atcagcagtt aacgcaatca ttttgcgatg tggtggatgc cattattgct
    13681 ggtggtggga tggtcggtgg tctgggcgat cgttttacgc gtgtagcggc ggcccatgcc
    13741 gtacataacg gtctgactgt cctgccacaa accgagaagt ttctccacgg caccaaagtc
    13801 gcctacggaa ttctggtgca aagcgccttg ctgggtcagg atgatgtgct ggcgcaatta
    13861 accggggcgt atcagcgttt tcatctgccg actacgctgg cggagctgga agtggatatc
    13921 aataatcagg cggagatcga taaagtgatt gcccacacct tgcgcccggt ggagtccatt
    13981 cattacctgc cagtcacgct gacaccagat acgttgcgtg cagcgttcga aaaagtggaa
    14041 tcgtttaaag cctgacgtgc aaatttagca gcagcgcgcg ccgccttttc caccgtagcg
    14101 cgcgtcctgc cgctcacgga aaaactcttc gtaggtcatc ggcgtttgat cgggatggtt
    14161 aacccgcata tgttcgacat agttgtcgta atcaggcata ccaatcatca gcttcgccgc
    14221 ctgacctaaa tattttccgg cttttgccag tgaatcaaac atcttggcct ccgctaacca
    14281 tgttgctgaa agagtgccgg atgcggcgtg aacgccttat ccggcctaca aatgctatca
    14341 acgcgctcgg tcagccccct cgccccatcg gggagagggt tagggtgagg gggaacccac
    14401 ggcgccatgc caacattcgc caacatcccc cctcactctg actttagtgc gcgccttttg
    14461 cctgcaccac gatctcctcg acattttccg gcattggctc atacggcgtt tctttcgccg
    14521 ttggcttcgg ctctttcaat gccgcaagtg ccgtcttaat cgagaacagt gccagaacca
    14581 ccacgaccac cataaagaag atggttaacc cggcatccag acggttgttg aacaccagtt
    14641 gcgccagctg tgactcagta tactgcgatg gaatattacc gctgtcgatc attgcctgga
    14701 acttattagc aatggccagg aagccgactt tcgcatccgg gctaaacgct ttctgccagc
    14761 ctgcggtcag ggtacaaatc agcagccagg ccgttggtac cagcgccacc caggcgtaac
    14821 gttgacgttt catcttgaac aacaccacgg cacagagcat cagcgccatc cctgccagca
    14881 tctggttggc aataccaaac agcggccaca gagtgttaat accgcccagc ggatcgacca
    14941 caccctgatg caggaagtag ccccacgcca gcacgcacag cgccgttgcc agcaggttag
    15001 caggcagtga atcggtccgt ttcaggcccg gagaaaccac gcccagcaga tcctgcaaca
    15061 taaagcgcgc agcacgcgta cctgcatcca ccgccgtcag aataaacaga gcttcaaaca
    15121 gaatggcgaa gtgataccag aacgccacat ccatcatgcc gcccagcgcg ccatgcagaa
    15181 tgtaggccat ccccaccgcc aacgtcggcg caccgcccgc acgggaaatg atcgactgtt
    15241 ctcccacttc gctggcaatc tggttgagcg tatccggagt aatcgcaaag ccccagctac
    15301 tcaccacttg cgcggcagaa gcgaccacat ccgccgtccc tgccggagcc agcaccgcca
    15361 tcgggctgtt catggcgaag tacacgcccg gatcgatgat acaggcggaa accagtgcca
    15421 taatcgccac gaaggattcc attaacattc caccgtagcc gataaaacaa gcctgccctt
    15481 cgttcgccag catcttcggc gtggtgccgg aagagatcag cgcatggaag ccagacaccg
    15541 cgccacaggc gatggtgata aacaggaacg ggaacaggtt accggtccat accgggccag
    15601 tgccgtcaac aaatttggtc aacgcaggca tggtcagcgt cgggcgcata atcaaaatgc
    15661 ctaccgccag accaacgatc gtcccgattt tcaggaaggt agagaggtag tcacgcggag
    15721 ccagcagcaa ccataccggc agcaccgccg ccacaaaacc gtaacccacc agcatccagg
    15781 tcagctgcac gccggtaaag tcaaagtacg gtgcccaggt cggactttct gccacccagc
    15841 cgccagaaat aatggcgaaa atcaggaata ccagaccgat gaccgacact tcaccaatac
    15901 gccccggacg cagatagcgc aggtagatcc ccatgaacag cgccagcgga atggtgaacg
    15961 caacggtata cgttccccac gggctatgag tcagggcttt caccacgatc atcgccagta
    16021 ctgcaaggat aatgaccatg atcataaagc aggccaccag cgcgatcacc ccggcggttg
    16081 gccccatctc ttctttgacc agctcaccca gcgaacgacc gtcacggcgc gtagaaacaa
    16141 acagcaccat gaaatcctgc accgcaccgg cgagaaccac cccggcgagt agccagatca
    16201 tccctggcag gtaacccatt tgcgccgcca gtaccggccc aaccaacgga cctgctccgg
    16261 caatggccgc aaaatggtga ccgaacagca ctttcttgtc cgtcggcaca tagtccagcc
    16321 cgtcgttatg gcgcaccgct ggcgtcatac gcgtcgggtc aaccgccagc acatttttgg
    16381 cgatatacag accataaaaa cggtaagcga tcagatagat acagaccgac gccaccacaa
    16441 tccacagcgc gttgatctgt tccccacgat ttaaagcaat atatcccaga gcaaatgctc
    16501 ccattacaga gagcactgtc cagacgaggt atttccctga tttgttcata gttgttatcc
    16561 gtgtgcgtgt ccatagagat gttacatttt gtttctataa catctttgta cgatttaaca
    16621 acacagcaac catgtaaatc ggagttgaaa ccaatattta acttaacaat gttaactcga
    16681 tcactccgtt aaccgagttt tcctgcaatc tcatataatt ctgtcgggtt catcccaaaa
    16741 ctgccgtacc cagccgacaa gtgcagcaac gccgcccctg ttcatcgaaa acgacgattt
    16801 cccagctctg attttgccgc ccaagatgca gcggctggca gacgccgcgt accttgcctt
    16861 cagacaccgg gcgatgatgt gtggcgttaa gttccgtgcc caccacacac tgcccgtcgc
    16921 gggtcatcat aaatccggcc atcgatccca gcgtttcagc cagcgccgcc gacgcgccgc
    16981 catgcagcag gccaaacggc tgatgagtac ggatatcaac cggcatttcg gcttccagca
    17041 catcatcgcc cagacgggta tacacaattc ccagatgcgc caccattgtg ttatcgctgg
    17101 tggcgttcag ttcgtcgagc gttaaatggc gtttccagat catgcttatg cccccagcgt
    17161 tgagccgcca tcgaccacaa tatcctgtag ggtgatatgg ctggcgaggt cagaggcgag
    17221 gaacaaaatc gtgttggcga tctcttgtgg acgggcgatt ttccccagcg gaatgccgag
    17281 tttaaactgc tcgccaaagc cgcgaatacg ctgttcttcg gcgtcatcgc tcacccacag
    17341 cgtgcgttgc atatcggtat cggtggaacc cggcgaaacc acattacagc gcacgccgct
    17401 acccgccagt tccagcccga cgctcaacgc caggcttttc agcgccgctt tcgatgcgcc
    17461 ataagcactc ataccaatac gcggcgtgtg tgcggcgtcg gacgccacag tgacaatcgc
    17521 cccgccccgc tgacggcgaa actggttcat ggtttgctgg aacaggttaa acgcaccgcc
    17581 gacattaacc gcaaaagtct gctgccagtc ctccttactg agctgatcgg tcgcgcccat
    17641 gcgcaaaatt cccgccgcat tgaccagcac gtccagtcgc tccgtttcag ctaacagtcg
    17701 ctgacatacc tgcgccacct gcgcagcatc ggcaacatcc attacttcgg tcgcaaaggg
    17761 atattgctcc tgagcgaacg cttgatcaaa acctgtaact ttcgctcccg cttcaacaaa
    17821 cgccagcgcc gtggcgtagc cgataccttt ccccgcgccg gttacccaga catttttacc
    17881 gctgaaatcc attatttcac ctcgcgggag agtagcttcc accaggcgtc gatggtcggg
    17941 tttttcgcca acatgacaaa gtcgatatcg ccatgcactt tgcgccagcg cgccgccagc
    18001 gccatcatgc gcaccgaatc cagaccgtag tcgatcaggt tgtcgtcatc gaacggttca
    18061 tcggactcgt ccagcaacgg caggatcacc tcacgcagcg ccgctttgct ggcggggata
    18121 ggtgctggca gtaattcttc ggtcatcacc acccggccag aacgtccggc cacatatttc
    18181 agcgacatca aatgctcgtc acggctgaaa tcggccagcg catccgccac cataaacggt
    18241 ttaatatcgc gcataaatgc gtcggttgcg gtggtcatac agccaatgtg ggcatatacc
    18301 ccggtaataa tcagctggtt acgtccactc tctttgagca tttgctccag cggagaacga
    18361 tgaaacgcgc tgtagcgcca cttcaccagc accgtgtcgt cggcatctgg cgtcagtcga
    18421 tccaccacct tttgctgttc cggcgaacgg gtcaggcccg gcccccacat atcattcaac
    18481 agcgcccgat cttcatcgct ctgctctttc ggctgggcgg tgtaataaac cgggatattg
    18541 tgctgtttgc agtagtcgcg cagcgcagca atattcgcga tcacctgctc catcatcggg
    18601 cagttctcgc cccagaagct gacaaaatag tcctgcatat catggattaa caacgcggca
    18661 cgttgcggtt caaaggccca gtcaacttta ttctgcggaa tatcgtgaga ctccggcagt
    18721 gcgtaagcct gtaattttgg aatagccatc gtgttctctc cttcaggctg atgcgcgtga
    18781 cgccagccac tgacgtaatt gttttttatc gactttcccg accgccgtca gcggaagtga
    18841 atccacacac tccacgcgat ccggtaattt aaattcggca ataccctgtt cacgcaggaa
    18901 acgacgcacc tgcaccgcgc gaagcggctc ttttaccacc agataagcac agcttttttc
    18961 gcccatcagc tcatcttcca tactgaccag tgcggcgtag atcaccgccg gatggcgcag
    19021 taacaggttt tcgatctctt cggcagcgat cttctcgccg ccacggttga tctgatcttt
    19081 ctcgcgcccc tgcacggtga tgtaaccctc tggatcaata gagatcagat cgccggaaca
    19141 gtaaaaaccg ttggcatcaa aggcgctggc attgtgctgc gggcttttgt aatagccacg
    19201 gaaggtatac ggcccgcgcg tcatcagtcg tccgacttcc ccttgcggca gtggatttcc
    19261 ttcggcatcg gcaacccata cttcgtcatc cggacacatt gggtaaccct gggtatggat
    19321 aattttctcc gcgctatcat caagacgggt atagttcacc agcccttccg ccataccaaa
    19381 cacctgctgc aactggcaac caatctcagc gggaatacgc gccgcaagcg tggcagaaag
    19441 acgtgcgcca ccgacctgta acagtttcag cgaggcaagc tgcgcccggc tttcgccttc
    19501 ggtcagcgcc tgcaaccaca ggctgactgc cggcggcacc agcgcggtaa cgttaatctg
    19561 atgtttttca atcaatggga agcaaagcgt ggcgctggga tcggccgcca gaacaaccgt
    19621 tcctccggca agaaagacgc ccagcgatcc cggcgaactc atggcgtagt tatgagccgc
    19681 cgggatcgcg cacaggtagc gtgtctgttg tgtgaactga caaatctcga cgctacgacg
    19741 cacgctgtag tagtagtcgt tatgagtgcg cgggatcagc ttcggtgtac cggtggtgcc
    19801 gccggaaagc tggaaataga ccacttcatc agcaggtgac ggcgtagcag taaaaccgtc
    19861 tgccggatgg ttaatcgcat cctgcaagtt atgctcaccg ctgtcgttga gtagctgcac
    19921 cacgcgaatg gaagaatgct ctgcaacaaa tgtgttgagg aaatcatccc cgctaaacag
    19981 cgcatgttgg cgatcggcaa tcagcaatgc gggttcaatc tggctggcat aggcgttcag
    20041 ttcactacgc tgatggctga acaacgccag caccggcgca acgcccagtt tcagcagcgc
    20101 gaaaaaggta atgtaaagtt cagcgacgtt acccagttgt accagcgcgg tttcaccagg
    20161 tttaatgccc tgacggcgta aactacacgc gaggttatcc gccgcctgat tcagctcccg
    20221 gtaactcaac tgtcgctcgc cgtcgataac cgcgatgctg tcactcgcag cgtggcgagt
    20281 cagaatgtca gtcagtggca aatcctgcca gtagcctttt tcccgatagc gacgggcaaa
    20341 ctcttccggc cagcgggtga atggaatgct catcctcgct ccttaatgca atccaaaaac
    20401 gttcaacatg gtagaaagtt tgacgcctgt ttcgcgccac tcacccaacg gtgacgacgc
    20461 aggcacaatc cccgctccgg caaacagacg cacctgattt tcccgcagct tcgcgcagcg
    20521 gatggtcacc acccattcgc cgttaccttc gctgtcacac caacccacaa tgccgccaaa
    20581 cagttcgcgg tcgaacggct ccagttcagc aataacctgg gtcgcggcct gatgcgggaa
    20641 accgctcagc gcgggggtcg gatgcagcag acaggccaga gtcagtgcgt tttcttgcga
    20701 attcgcttta ccttcaaagg gagttgcgag atgccacagc gtcggcgtgg taatcaattg
    20761 tggagaggaa ggaacgtgta actcactact gcgttcgcgc agtacctctt tcatcgcctg
    20821 agtcaccagt tcatgttcat ggcgatcttt ttctgacgcc agcagacgat tacccgcttc
    20881 gcgatcgagc acttcatccg gctgacgacg tgcggaaccg gctaacggaa tggagctaaa
    20941 acgctcaccg tctttacgta gcagcagttc cgggctggcc cccagcagaa cgccaccatc
    21001 tgccagcggg acatggaagt tgtaactaac cgggttttgc gcaatcaacc gttccagcaa
    21061 tacgccacta tcaatggcgg cgtcagtggt gatatcaatc aaccgtgaca acaccacttt
    21121 gtcgacctgc ggcgtggcgg taagtgcggc ggcgcgggca accatctgtt caaacgtggt
    21181 ttgctccgga attgcctggc gttccaccac gttcagcgac tggctgcggg tgaaacggcg
    21241 tgcggaagct tgtttttcct gacgggagaa cgactgccag gattcaggaa tatacagcga
    21301 cgaaggctga cgtggatcga aaggaatcgc cccgaccatc accggatttt tgatgccctg
    21361 cgctttggca tcggcaaaca gcgcggcgag tttttgctgg aagggactgt cgggcgaatc
    21421 cccgttcaca gccggttcat cgaagcgggc gaaacatcct gacgtcgtaa aactgcggta
    21481 cggcgacata aagaaaaagc gattgggcgc aagtgttgcc atggtctgct gtacttcctc
    21541 agccagtgac gtatccatat catcctccac aaaatgataa aggctttaat aatgattatc
    21601 atttatattt tcggtcgcta acctaaaagc aaacgcgcac tatgtcaact cttgaggtaa
    21661 cgcaatctgc aactcggggt tgcatctggt tgtggttata atgaaaatga gaagcattaa
    21721 cttcattaat aacaggaggc tgatttgtga gactcgcccc gctctaccgc aatgcccttc
    21781 tattaacagg acttttgttt tcaggaatag ccgcagttca ggccgccgac tggccgcgtc
    21841 agattactga cagccgtggc actcataccc tggaaagcca gccgcagcgt attgtttcca
    21901 ccagcgtcac cctgaccggc tcactgctgg cgattgatgc tccggtgatc gccagcggcg
    21961 cgaccacgcc gaataaccgc gtcgcggatg accagggctt tttacgccag tggagcaagg
    22021 tggcgaaaga acgcaaactg caacggctct atatcggcga accgagcacc gaagccgttg
    22081 ccgcgcaaat gccggatctg attttaatta gcgcaaccgg cggggattcg gcgctggcgc
    22141 tgtatgatca gctttccacc atcgccccga cattaatcat caattacgac gacaaaagct
    22201 ggcaggcgct gttaacgcaa cttggcgaaa ttaccgggca tgagaaacaa gcggcagagc
    22261 ggattgcgca gtttgataag caactggcgg cggcgaaaga gcaaatcaaa ttaccgccgc
    22321 agccggtcac tgccattgtc tatactgccg ctgcacacag tgccaatctc tggacgccgg
    22381 aatcagcaca agggcagatg ctggaacaac tcggctttac gctggcgaag ttacccgcag
    22441 ggttaaatgc cagccaaagc cagggtaaac gccatgacat cattcagctt ggtggggaaa
    22501 atctggctgc agggttaaat ggcgagtcac tattcctgtt cgccggtgat cagaaagatg
    22561 ccgatgctat ttatgctaat ccgctgctcg cgcacctgcc tgcagtacaa aacaagcagg
    22621 tttatgcgct gggaaccgag acgttccgtc tggattacta cagcgccatg caagtgctgg
    22681 aacgacttaa ggcgctgttt taagcattaa ccgtcagacg ctgtcacctg cggcggcgtc
    22741 tggcgaaaac gtcgcaactc caccagcacc agcaataaca acacgccgat aatcaataaa
    22801 ccaaaaccgc tcgcgcttgc ggaagcaacc ggtgtcatca tcgctcccaa accacccagc
    22861 agcgccgcgc ctatggcatc gcccgtcacg ttttgtgccg tccacaaacc gttaatccgc
    22921 cctaacatcg cttccggggt ttgcgtttgc agcattgtgt attgcagcaa cgagctgacc
    22981 gcactcaacc agccgaacag cgccagacaa accacgccta aaatccacat cggcatcagg
    23041 ccaaacagac caatggcgag gaacgatccc agcgtggaga gcagcatcaa taaccctggt
    23101 cgcgcgctat gcgccagctt cccgctggtt aacgcgccaa tagccgcgcc gagcgggatc
    23161 gccgcataga gaaaaccaat ctgtgccgcc gacatctgcc agttgtcagc cagcgccgga
    23221 tacagtaccc gcaccgcact cgccatcgtc aataaaccac ccagcagcgc aatccctccc
    23281 accagcgggc tggcgagtag aaaacgaaat cctgccagta atgatttcaa cggatgctca
    23341 cgcggctgcg gtggcggtgg cagtgccgga aggcttaaca acggtagcaa ggtaataaac
    23401 gtgcccgccg ccgccagccc gtagttccag gccacaccgc cagtcgccag cagtaaaccg
    23461 ccaatcatgg gcgaaatcac cgaccccaga cgcacggtca acatggtgat cgccccggcc
    23521 tgcattaagt tttcacgccc taccagtgct ggtgtcgccg ccaatagcgc cgtaacgcca
    23581 agcgatgcga aaaaaccatc ccataaacca agtaaataga ttgccagcaa tgacggctcc
    23641 ggcagcagcg cgttcaggca cagtccaatg aagccaatgc cacaggtgcc gcgcgccagc
    23701 aaaatcactt ttttgcgctc atagcgatcc gccagcacac cgccgaccat caggccaaca
    23761 aacatcgcgc cgccggtcag cgtcaccgaa agccccacct gccaggtgga atgtgtcatc
    23821 atctggatct gcaccggcac cgcgacgccg agcaaaccca gagacacaat tgagatgaaa
    23881 cgagcgagga atactgcgcg aaacgccggg tgcgttttca acaggctgag gttaagcagc
    23941 caggattgtt tattcattac aatgccttgc catcaaatat tttaaatacc tattcttagg
    24001 ctgcacatgc taacatatcc aaataagatc gataacgata attaatttca ttatcatgga
    24061 agttcgtatg tctggttctg ttgccgtgac acgcgccatt gccgtgcccg gattgctgtt
    24121 attactgatt atcgcgacgg cattaagcct gctcattggg gcaaaatcac tccccgcttc
    24181 cgtagtgctg gaggccttct ccggcacctg ccagagcgcc gactgcacca tcgtgctcga
    24241 cgcgcggctg ccgcgtaccc ttgccggttt actggcaggc ggcgcgcttg gccttgccgg
    24301 ggcgttaatg caaaccctca cccgaaaccc acttgccgac cccggcttgc ttggcgtgaa
    24361 cgccggagcc agctttgcca ttgtgctggg tgcggcgctg tttggttact cttccgcaca
    24421 ggaacaactg gcgatggcct tcgccggggc gctggtggcc tcgttgattg ttgcctttac
    24481 cggcagccag ggcggtgggc agttaagtcc ggtgcgttta accctggcgg gcgtggcgct
    24541 ggcggcggtg ctggaagggc tgaccagcgg catcgccctg cttaatcctg acgtctacga
    24601 tcaactgcgt ttctggcaag ccggttcgct ggatattcgc aatctacata ccttaaaagt
    24661 ggtgctgatc ccagtgctga tcgccggagc aactgcgcta ttactgagtc gcgcgctgaa
    24721 cagtttaagc cttggcagcg acaccgcgac ggcgctgggc agtcgcgtgg cgcgcacaca
    24781 gttgattggt ctgctggcga ttaccgtgct ttgtggtagt gcgacggcgg tagttggccc
    24841 tattgccttt attggcctga tgatgccgca tatggcgcgt tggctggtgg gtgccgatca
    24901 tcgctggtcg ctgcccgtca cgctacttgc tacccctgcc ctgctgctgt ttgccgatat
    24961 catcgggcgg gtgattgtgc ccggtgaact gcgcgtttct gtggtcagtg cgtttattgg
    25021 tgcaccggtg ctgatcttcc tcgtgcgacg taaaacgcga ggtggtgcat gatttacgtc
    25081 tctcgccgat tactcatcac ctgtttgctg ctgatttccg cctgtgtggt tgcaggtatc
    25141 tggggattac gcagcggtgc cgtcacgctg gaaatctcgc aggtattcgc cgcgctgatg
    25201 ggcgatgcgc cgcgcagtat gacgatggtg gtcaccgaat ggcgtttacc acgcgtgctg
    25261 atggcgctgt tgattggcgc ggcactgggc gtcagcggtg cgatttttca gtcgctgatg
    25321 cgtaacccac tcggcagccc tgacgtaatg ggctttaaca ccggggcgtg gagcggcgtg
    25381 ttggtggcga tggtgctgtt tggtcaggat ctgacggcta tcgcgctggc agcaatggtg
    25441 ggcggcatta tcacttcgct gttggtctgg ctgctcgcct ggcgtaacgg tatcgacacc
    25501 tttcggttga ttattatcgg tatcggcgtt cgcgccatgc tggtggcctt taatacctgg
    25561 ctgttgctga aagcgtcttt agaaacggcg ctaacagcag gtttgtggaa tgccggatcg
    25621 ctcaacggcc tgacgtgggc aaaaacctcg ccttccgcac ccatcattat attgatgcta
    25681 attgccgccg ccttactggt acgccggatg cgcttgctgg aaatgggcga tgataccgcg
    25741 tgtgcgctgg gcgtcagcgt cgaacgttcg cgtctgttaa tgatgctggt tgcagtggtg
    25801 cttaccgctg cggcaacagc gcttgccggg ccgatttcct ttattgcttt agtcgcgccg
    25861 cacattgccc gacgcattag cggcaccgct cgctgggggc taacccaggc ggcgctatgc
    25921 ggggcgctgt tactgctggc ggccgatctc tgcgcccaac aactgtttat gccgtatcaa
    25981 cttccggttg gcgtcgttac cgtcagcctc ggcggtattt accttatcgt tttgttaatt
    26041 caggagtctc gcaaaaaatg accgaatcag tagcccgttt gcgcggcgaa cagttaaccc
    26101 tgggatatgg caaatatacc gttgcggaaa atctgactgt agaaatacct gatggtcact
    26161 tcacggcaat tatcgggcca aatggctgcg gtaaatccac gttgctgcgt accttaagcc
    26221 gcctgatgac gcctgctcat gggcatgtct ggctggatgg cgagcacatt caacattacg
    26281 ccagtaaaga agttgcccgg cggattggtc tgttggcgca aaatgccacc acgctgggag
    26341 atatcaccgt gcaggagctg gtggcgcgtg ggcgttatcc acatcaaccg ctgtttaccc
    26401 gttggcgcaa agaagatgaa gaggcggtaa cgaaagcgat gcaggccacg ggaataactc
    26461 atcttgcaga tcaaagcgtg gatacccttt ccggtgggca acgccagcga gcgtggatcg
    26521 cgatggtgct ggcccaggaa acggcaatta tgctgctcga cgaacccacg acctggctgg
    26581 atatcagtca tcagattgat ttgctggaac tgttaagcga actgaaccgc gagaaaggct
    26641 ataccctggc ggcggtgctg cacgatctta atcaggcctg tcgttacgcc agccatttga
    26701 ttgcattgcg agaagggaaa attgttgctc agggagcgcc gaaggagatt gtcactgctg
    26761 aactgattga gcgcatttat ggtctccgct gcatgatcat tgacgatccg gtagccggaa
    26821 cgccgctcgt ggtgccgctc ggacgaacgg caccttcaac cgcaaatagt taaactaagt
    26881 ggtctgccat catggcatcc tgttttctgg atgccatcgc atagcgcaat aacacgctac
    26941 cacaagccac catcccgccg atcagcgcgg aaaggatcac aataatcgcc ttacccggac
    27001 cgtctttttt cactggcaat gacgggctta actgatattt aaacggcgta aaattcacat
    27061 cgttgatatt tgcttttgtt aactgctcaa caagatactg acgattacgt aattcaccgt
    27121 tcagttccgc aacgtcagtg accgcttttt caatttccag tttgcgttca ataccgtctg
    27181 cgccgagaga aatagagaaa tcggggtcat ctttaacggc ctgaccatta ctgtaaacgg
    27241 gctttttaat tcctgccgcg ttggcaatgt cgagtgaata attgaggcgc tgaatgtttg
    27301 catcaagttg atttttcatt ttaatgcggt cctgagccag tttttctttt tcaaactggg
    27361 ttttgatctc cagtttatta cggacgtttt ctatcgactc tttcaccacc aacgcagaga
    27421 tataatcgat atacccgctc aaaacggtct gcgcctcttc actggttggc gcggtaaaac
    27481 ttagcgtcca ggaggtatac agtgacggtt catctttttt cttactggca ttgtcatcaa
    27541 ccgctttcat tttttcgctc aatgcgacaa ttgcgcgatg caaatccagt tcgtcgattt
    27601 tcgcctcttt taattggtcc atcacataag gtgatgaacg caggtactct tccagcaagc
    27661 taaccgactg aaacttcttg ataaacaggt taaatgcttc tgtacgatca attttgatat
    27721 ccagatccag cacacgaagc ttagtgaatg ttttctccaa ctcttgccac tgaacaggtt
    27781 ctggaggcgt gacaaccgcc gcgctggtcc atttttgcgg caggatgaaa gagatcagca
    27841 agcctgcaca ggcaaacgca aaaacgaccg ccatgaccgt ttttttggcc cgccataaaa
    27901 ctgagattag atta
//
LOCUS       DAZFBR010000063        27904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFBR010000063 DAZFBR010000000
VERSION     DAZFBR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.063"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            74..736
                     /gene="acrS"
                     /locus_tag="ACHJZ2_002854"
     CDS             74..736
                     /gene="acrS"
                     /locus_tag="ACHJZ2_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO4568931.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(739..918)
                     /locus_tag="ACHJZ2_002855"
     CDS             complement(739..918)
                     /locus_tag="ACHJZ2_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO4568932.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(1002..1886)
                     /gene="yhdJ"
                     /locus_tag="ACHJZ2_002856"
     CDS             complement(1002..1886)
                     /gene="yhdJ"
                     /locus_tag="ACHJZ2_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4568933.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            complement(1971..2267)
                     /gene="fis"
                     /locus_tag="ACHJZ2_002857"
     CDS             complement(1971..2267)
                     /gene="fis"
                     /locus_tag="ACHJZ2_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO4568934.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2293..3258)
                     /gene="dusB"
                     /locus_tag="ACHJZ2_002858"
     CDS             complement(2293..3258)
                     /gene="dusB"
                     /locus_tag="ACHJZ2_002858"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO4568935.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3587..4468)
                     /gene="prmA"
                     /locus_tag="ACHJZ2_002859"
     CDS             complement(3587..4468)
                     /gene="prmA"
                     /locus_tag="ACHJZ2_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO4568936.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4480..5931)
                     /gene="panF"
                     /locus_tag="ACHJZ2_002860"
     CDS             complement(4480..5931)
                     /gene="panF"
                     /locus_tag="ACHJZ2_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO4568937.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5921..6163)
                     /locus_tag="ACHJZ2_002861"
     CDS             complement(5921..6163)
                     /locus_tag="ACHJZ2_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO4568938.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6272..7621)
                     /gene="accC"
                     /locus_tag="ACHJZ2_002862"
     CDS             complement(6272..7621)
                     /gene="accC"
                     /locus_tag="ACHJZ2_002862"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO4568939.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7632..8102)
                     /gene="accB"
                     /locus_tag="ACHJZ2_002863"
     CDS             complement(7632..8102)
                     /gene="accB"
                     /locus_tag="ACHJZ2_002863"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO4568940.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9080..10054)
                     /locus_tag="ACHJZ2_002864"
     CDS             complement(9080..10054)
                     /locus_tag="ACHJZ2_002864"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO4568941.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10206..12146
                     /gene="csrD"
                     /locus_tag="ACHJZ2_002865"
     CDS             10206..12146
                     /gene="csrD"
                     /locus_tag="ACHJZ2_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO4568942.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12451..13494
                     /gene="mreB"
                     /locus_tag="ACHJZ2_002866"
     CDS             12451..13494
                     /gene="mreB"
                     /locus_tag="ACHJZ2_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO4568943.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13560..14663
                     /gene="mreC"
                     /locus_tag="ACHJZ2_002867"
     CDS             13560..14663
                     /gene="mreC"
                     /locus_tag="ACHJZ2_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO4568944.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            14663..15151
                     /gene="mreD"
                     /locus_tag="ACHJZ2_002868"
     CDS             14663..15151
                     /gene="mreD"
                     /locus_tag="ACHJZ2_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO4568945.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15160..15753
                     /gene="yhdE"
                     /locus_tag="ACHJZ2_002869"
     CDS             15160..15753
                     /gene="yhdE"
                     /locus_tag="ACHJZ2_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO4568946.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15743..17212
                     /gene="rng"
                     /locus_tag="ACHJZ2_002870"
     CDS             15743..17212
                     /gene="rng"
                     /locus_tag="ACHJZ2_002870"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO4568947.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17280..21080
                     /gene="yhdP"
                     /locus_tag="ACHJZ2_002871"
     CDS             17280..21080
                     /gene="yhdP"
                     /locus_tag="ACHJZ2_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO4568948.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21236..22681
                     /gene="tldD"
                     /locus_tag="ACHJZ2_002872"
     CDS             21236..22681
                     /gene="tldD"
                     /locus_tag="ACHJZ2_002872"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO4568949.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22815..23744)
                     /gene="aaeR"
                     /locus_tag="ACHJZ2_002873"
     CDS             complement(22815..23744)
                     /gene="aaeR"
                     /locus_tag="ACHJZ2_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO4568950.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPSLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23927..24130
                     /gene="aaeX"
                     /locus_tag="ACHJZ2_002874"
     CDS             23927..24130
                     /gene="aaeX"
                     /locus_tag="ACHJZ2_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO4568951.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24138..25067
                     /gene="aaeA"
                     /locus_tag="ACHJZ2_002875"
     CDS             24138..25067
                     /gene="aaeA"
                     /locus_tag="ACHJZ2_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO4568952.1"
                     /translation="MKILIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25073..27040
                     /gene="aaeB"
                     /locus_tag="ACHJZ2_002876"
     CDS             25073..27040
                     /gene="aaeB"
                     /locus_tag="ACHJZ2_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO4568953.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27132..27404
                     /locus_tag="ACHJZ2_002877"
     CDS             27132..27404
                     /locus_tag="ACHJZ2_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO4568954.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27460..27723)
                     /gene="yhcN"
                     /locus_tag="ACHJZ2_002878"
     CDS             complement(27460..27723)
                     /gene="yhcN"
                     /locus_tag="ACHJZ2_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO4568955.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6708 a         6962 c         7426 g         6808 t
ORIGIN      
        1 cattaggtga tagattaacc ttcgctattt tctcactctg tgtcgaagat atttatttcc
       61 tgaataatta atcatggcaa aaagaaccaa agccgaagct ctgaagaccc ggcaagaact
      121 gattgaaact gccatcgccc agtttgcgca acatggcgta agcaagacga cgctcaacga
      181 cattgccgat gccgctaacg ttacgcgtgg cgctatctac tggcacttcg agaacaagac
      241 ccaactgttt aatgagatgt ggttgcaaca gccttcattg cgggagttaa tccaggaaca
      301 cttgacggct ggattagagc atgacccgtt tcaacaattg cgtgaaaaat tgattgtcgg
      361 cttgcaatat attgccaaaa ttccccgcca gcaggcgttg ctgaaaatct tatatcacaa
      421 atgtgaattt aatgatgaga tgctggccga gggagtgata cgcgaaaaga tgggctttaa
      481 tccgcagact ctccgcgaag tattgcaggc gtgtcagcaa caaggttgtg tagcaaataa
      541 cctcgattta gatgttgtga tgattattat tgatggtgcc ttcagcggaa ttgttcaaaa
      601 ctggttaatg aatatggcgg gttatgatct ttataaacaa gcccccgctc tggtcgataa
      661 cgtattaaga atgttcatgc cagatgaaaa cataacgaaa ttaattcatc aaacgaatga
      721 attaagtgtc atgtaaattc acattctgtc ctgaaaattc agttcgtcac agttaaccag
      781 ttttagtgga ttaactgagt tcatattgcg gcatttgtct gtttctgttg ccagcagttc
      841 aatccactgc atcagtaaag tatcaaacag aaaaacgaag acagcgaaaa cgacgagcca
      901 ccaatacttg cgaatcatta cctgagcctg tgcatgttta accaagtctg gcaggaaata
      961 tacacgaaat gaataatctg caaaccaggc ttacacatcc tttactttgt aatgagatcg
     1021 gggtcaactt cgcttaaccg gcttcgtttt gacaggttgc cggtctttct ttttttcact
     1081 ttcgccagtt cttccgcaga gtaatgcgac gcgacatcca gccgtcgaag ccccattttg
     1141 atgtactcgc tgttgatctc aataccaatg aattttcgtc cgctggcgat ggctacggca
     1201 ccggtagtaa agctaccagc aaacgggtcg agaacgatat cgcctgggtt ggaagaggcg
     1261 agaataatgc gtttcagtaa ggcttccggt ttttgcgtcg ggtggttttc atattcatcc
     1321 atcaaataac gcacgcgcgg aaaatcccag acgttacccg gtactttttg atgattgtaa
     1381 ggctgtggag gatttttgcg ataatcgatc aacgcgcgct gcgatccggt tttggcttct
     1441 accagaatag catcaccgtt gaatgtgtag ttctttgcgt ctttcaccat catcaggatg
     1501 ggttcgtaca tggagccgta gtgttttttc gcctgcactc cagaactgtc atatgaccag
     1561 acgatgcgac ttttgatggt aaaaagcttg cggcactgga gatcgataaa gggcatgttt
     1621 tccgtactgt tcatgatgta catgctgccc tgctttttca gaacgcggtg gcactctgca
     1681 atcacttcta acagccagtc gataaacaga tcttctttcc aggcttcgat cagaccatca
     1741 aaatttttac cgatgttata cggtgggtcg gcaaagatca gatcgacgct ttcagtaggt
     1801 agctttttaa gttcggcaag ggcatcaccg tgaataatgg tcttagcttc attaccaaac
     1861 cgggtcggtt cacatcctgt tctcatggtc actccctttg tgacacctat aaaaaaggcg
     1921 cttccccatg ccgagtagcg ccttttaatc aagcaattag ctaacctgaa ttagttcatg
     1981 ccgtattttt tcaatttttt acgcagcgta ccacggttga tgcccatcat cagcgcagca
     2041 cgggtctggt taccacgggt gtattgcatc accatgtcca acaggggctg ttctacttca
     2101 gccagtacca gctcatagag gtcattcaca tcctgaccat tcagttgagc aaaatagttc
     2161 ttcagtgcct gtttaaccga gtcacgcagg ggtttttggg ttacctgatc ctgagagtta
     2221 acggtagaaa cggtcagtac gtcagaattt acgcgttgtt cgaacatagt tctgtcagct
     2281 ctttatttct gtttacgcaa aattttcgaa gtatgcctcc aacgcctcca gctgttcgct
     2341 ggcatcctca atggcgttga atgtgcgccg aaactggtca tttggagcgt gttcctggag
     2401 ataccaggaa acgtgtttac gtgcaattcg gtaccctttt gccggaccat aaaagtcatg
     2461 cagttcccga acgtgcgcgc aaagcaagcg cttaacctct gccaaaggca gcgggggcag
     2521 caactcccca gtgtccagat aatgctggat ttcccgaaag atccagggtc ttccctgagc
     2581 tgcgcggcct atcatcaggg catccgcccc tgtatagtcg agcacagctc tggctttaag
     2641 cgggtcagta atgtcgccat tcgcgataac cggaatggaa actttctgct taactgcccg
     2701 aatactgtcg tactcagctt ctccattgaa caaacaggcg cgtgtacggc catgaatggt
     2761 cagagcctga atgccacagt cttcagccag ttgggcaatc tcttcgcagt tacggtgttc
     2821 cggtgcccag ccggtgcgaa tcttcagggt aacaggaacg tccactgcat tgacgacctc
     2881 ggtaaggatc gatttaacga catccgggta ctgcaagagg gctgaacctg cgagcttgcg
     2941 attcactttt ttagccgggc aacccatatt gatatcaata atctgggcac cgctttccac
     3001 gttaatacgt gctgcatctg ccatttcttt cggatcgcta ccagcaattt gcacggtgcg
     3061 aataccgggt tcatcaatgt gcaccatccg taaacgagat ttgtcgcttt cccaaacctg
     3121 tgggttagaa gacatcatct cggatactgt caatccggct cccatctcgt agcacaacgt
     3181 ccgaaaaggt ctgtctgtaa tgccagccat gggcgctgcg atcaggcgat ttctgagctg
     3241 atattgtccg atgcgcatga gttaagaaat gaccatactg tgactgcaag gcggcgtata
     3301 ttacgcattt tttgcacgag atgaaaggcc aaactttgac caatcctctg agatggatca
     3361 aagaattgca tttaaaatga gcgtggtgcg ataattactc ataaaaatca tcatattaga
     3421 aaatagtgac taaaatttac actcaaagaa atttgagtaa gttctcaatt tttctttatg
     3481 aatgaaaatt ttggcacgca aatttgcgta aataatcggc aaatttacgt gccttttgtg
     3541 agcttgctcg cacttcgccc cgcgtcaccc tatggcgatg cgaaggttaa ttcttacgac
     3601 cggtaatacg gcaccactct tctttttcca cgaccgggtc cagagcgaag ctgtcggcat
     3661 aggcttcgca aacgctctcc gcctggcttg ccagaatacc agaaaggccc agtaagccgc
     3721 ctgaaactgg caggacgcta attaacggtg ccagttcacg taatgggcct gcaaggatgt
     3781 tagcgaccac cacgtcggct ttcatttctt ctggctgatc tttcggtaag tagagttcca
     3841 gacggtcaga aacgccatta cgttcggcgt tatcgcggct ggcctgaatc gcctgcggat
     3901 cgatatcaat accaatggct tttgctgcac ccagtttcag cgccgcgatc gccagaatgc
     3961 cggaaccaca gccaaagtcg atgactgttt taccggttaa atcgaggctg tcgagccatt
     4021 gcaggcacag agaggtggtt ggatgggtac ccgtaccaaa cgccagccct ggatctaaca
     4081 tcacgttgac ggcgttttcg tccggcacat cacgccagct agggcagatc cacagtcgtt
     4141 cgccaaagcg catcgggtgg aaattatcca tccattcgcg ctcccagtct ttatcttcta
     4201 gttgttcgat tttatgcgcg aagcctgcgc cgagcagcgg atggttttcc agaatcgcca
     4261 ccacgtcgtt catatcggtt tcagcgtcga acagaccaat cacatcggtg tcgccccaca
     4321 ggcgcgtttc gcccggcagc ggctcaaata ctggcgtatc gtgggtatcc tgaaaagtga
     4381 tagaaacggc acccgcttcc atcagcgcat cgctaagatc ttccgcgttc gcgccggtgg
     4441 tgttcagttt cagttggatc caaggcatgg caaaactctt tatttatcag tagtcaaaac
     4501 ggtagcttgc gggacggatg taccgaaacg gtttccgacc aggaaagcca gcaaacttag
     4561 tagtaacgag ggcacgatag ggtggaagcc caggtactga atattcagcg tcgcgagtac
     4621 ggcatacagc acgccgccaa cgatcatcgc acttagcgcg cctttggcgt tggcgcgttc
     4681 ccagtaaaga cccagcacca gcggccacag gaaaacggct tccagcccac cgaaggccag
     4741 caaattcagc cagatgatca tttctggcgg cttccaggcg gcaagcagca gcaacgcgcc
     4801 gagaactaac gtaattaccg ccgacatccg cttcagacgc gtctcgtttt gcatttgatc
     4861 cggacggata ttcagataga gatctttaat gatcgtagcg gaactttgta gcaattgggc
     4921 gttaattgtc gacatgatcg cagccatagg tgcagccaga aagatcccgg cagcaaacgg
     4981 tggcagcact tttaccatta acgtcgggat caccagatcc ggtacggtga gatcggggat
     5041 cactgcacga cctaacgctc cggctaggtg cataccgaac atcaggattg cgactacaat
     5101 cgtaccgatg atgatccccc ggtgtactgc tttgctgtct ttataagaga tacagcgcac
     5161 cgcagtatgc ggcaggccta taacgccaaa gcacaccagt acccagaacg acgtcataaa
     5221 ggcaggcgac agaatatcgt cagcgccttg tggcgtaacc agttgcggat cgatggtttg
     5281 caaggtctgt actgcgttgc ttaagccgcc agcggcatgt accacgccaa taagcagcac
     5341 aacggtgcca atcagcatca caagcccttg catggtgtcg ttcagcacgc tggcgcgaaa
     5401 gccaccaaag gcggtatata acgcaatgct gataccaaaa atcagcagcc cggtttcata
     5461 aggaataccc gccgcggttt ccagcaggcg cgcaccgccg ataaactgca cggtcatcgc
     5521 accaacgaac gcaaccagca aactcaaact cgccagccac accagaagac gactctggta
     5581 gcgggcaaac agcatatcgt tcagcgtcac tgcattgtag cggcgcgcaa gaatcgcaaa
     5641 cttcttgccg agaataccga gtgaaagcca gactgccgga agctgaatca tcgccagcaa
     5701 tacccagcca aggccgtatt tgtaagcagc tccaggcccg ccgataaacg aactggcact
     5761 gatataagtc gcggtgagcg tcatcgccag cacaataccg cccatagagc ggctgccgag
     5821 gaaatactca ttaaggaagg tgccggtgct ccgtttacgc atcgcataaa ccgagatacc
     5881 gaacaccacc accagatagg cgaccagcgg tagaattact tcaagctgca tcgtcatcct
     5941 ccagcgggat atcgcgatag ataaatttca ccatcgccca gcacagtcca ataaacagca
     6001 gcggcgtcag gatgcaggcc atctcaaacc agcgcggaaa gccggtaaaa ccgggggcaa
     6061 cgccaggtaa gtaagcggct actaaccaaa ctgccagata caaaagggtc agccccagcg
     6121 cccagcgcgc ctctttatgg gcctgaacaa aacgagtgtc cattttttgt ccctgatggg
     6181 tgaagaaagc gaggattgta ccttatgggt gttgtcgatc cccagtaata aaaaaggccg
     6241 gaaaatccgg ccttttgacg cttttgcagt cttatttttc ctgaagaccg agttttttct
     6301 ccagatagtg gatgttagtg ccaccatgct ggaagttctc gtcattcatg atgcggatct
     6361 gcagatcaac gttggttttg ataccgtcga tgatcagctc ctgcagcgca ttcttcatgc
     6421 gggcaatcgc cacgtcacgg ttttcaccgt agcaaatcag cttaccgatc attgagtcat
     6481 agtacggcgg tacggtgtag cccgcgtaga tatgagactc ccaacgtacg ccaaaaccgc
     6541 caggtgcgtg gaaacgggtg attttgcccg gacttggcag gaaggtgttc ggatcttcgg
     6601 cgttgatacg acattccacc gcatggccgc gaacgtgaac ttcttcttgc ttgatcgaca
     6661 gcggttgacc ggcagcgata cgcagctgtt ctttgatcag gtcaacgccg gtgatcattt
     6721 ctgtaaccgg gtgttctacc tgaatacggg tgttcatttc gatgaaatag aactcgccgt
     6781 tttcgaacag gaactcgaaa gtacctgcac cgcgatagcc gatatcaaca cacgctttag
     6841 cgcaacgttc gccgatgtag cgacgcagtt ccggggtaat gcccggtgct ggtgcttctt
     6901 cgaccacttt ctggtggcgg cgctgcatgg agcagtcacg ttccgccaga tagatagagt
     6961 tgccctgacc gtcagccagt acctgaatct cgacgtggcg aggattttcc aggtatttct
     7021 ccatgtaaac catatcgttg ctgaaagcag ctttcgcttc cgcacgggtc atggagatgg
     7081 attgtgccag ttcagcgtcg ccgcgcacta cgcgcatacc gcgaccaccg ccgccgccgg
     7141 aggctttgat aatcaccgga taaccaatgc gtttagcaat ggcacggttt ttatccatat
     7201 cgtcgcccag cgggccgtca gaacccggta cgcaagggac gcccgctttt ttcatcgccg
     7261 cgattgcgga tactttgtcg cccatcaggc gaatggtttc tgctttcggg ccaatgaaga
     7321 taaagccgga gcgttcaacc tgctcggcaa agttggcgtt ctcggagagg aagccgtaac
     7381 ccggatggat tgctactgcg ccggtgattt cagcggcgct gatgattgcc gggatgttca
     7441 gataactttt tactgacgga gcagggccaa tacagaccgt ttcatctgcc agtaatacgt
     7501 gttttagatc gcgatccgcg ctggagtgca cagcgacagt cttgatgccc agttctttac
     7561 aggcacgaag aatacgcaat gcaatctcgc cgcggttggc aataacaatt ttatccagca
     7621 tgttcgcctc gttactcgat gacgaccagc ggctcgtcaa attctaccgg ttgtccactt
     7681 tcgaccagaa ttgctttcac ggtaccggat ttgtccgctt cgatctggtt catcattttc
     7741 atggcttcaa cgatgcacag ggtatcgccc acgttgactt tctgacccac ttcgatgaac
     7801 gcttttgcat ccgggcttgg ggtgcggtag aaagtaccaa ccatcgggga acgtacgatg
     7861 tgaccactga tttccgctgc tgctggcgct tccatggaag gaacggtcgc cggagcggct
     7921 gcgttagatt gagctggctg ctgcatcatt ggtgcagcgt aagcttgttg catcacaggg
     7981 aaacttgcgg caggagctgc acggctaatg cgtactgact cttcgccttc agaaatttcc
     8041 agttcggaga tgcctgattc ttcaaccagc tcgatcagtt ttttaatctt acgaatatcc
     8101 atgagtgggt tccgtactct ttgtttagtg tgattgtgac aggcgtttca ccgccgtctg
     8161 taaagcgtat gccccgacag ctgtatgcat agcgataaat tccagcaggt cgggacgccg
     8221 gtctattttg ccccagagtg tcaatgttag acttgacgga cattgtgcag cgatctgctg
     8281 ctatcctccg gcaaaaaaca aaatatacct tcgttgcgcc aatgtcacct tttccgcagc
     8341 gcaaaaactg cgtcagggag gacgaggtcg cacattataa cgatttcgta gcaattggca
     8401 gctaaatact ggtcttatca gggaagataa tcaacaacta acatgtaaat aaccttcaac
     8461 accgtgtaat ttgcaacaag ccgcacaatt cacgaaatta gcgccatcat cgacggaact
     8521 tcttataacg taaggtgaaa agctgtaaag ccagccctgc gaagatgagg ggctgcaatg
     8581 agataatcgt cacagaccac aaataatgta gggatgccag gatcgcgaca agatagacga
     8641 cattacgcag cggttgctgg ggattaccca attttcactg catcgcctgg atagatgtga
     8701 atgctaaagc acgcaaaatt aaccgattga caatgctcag cattaaatag gttcgagtga
     8761 ttaatttgta gtcaaacaaa atcagattat tcacctcatg gtaccagaaa taagtaaggg
     8821 gttaagcgta gcgtcgccca ggcaaagcac cataaaccta acaggcgacg gaggcgtatc
     8881 aataatagct gtttagtgga gtatcaatgc gcatcaattt atggtcagtc gaaatcatga
     8941 ctttccagtt tggatgatgt tgtacaactt catgaccaag gtgttcgacg aaagctgtcc
     9001 ggctaaccgc ttttcccgtt aaccgattga ataatgattt caaaatgagt tccctctctt
     9061 attattcctt gctaaatggt tagttaacct tcaccagcgt gcgaccctgg atctggttat
     9121 taatgatggc ctcggcaaag ttcggtgcct ctgacagaga tatctctttt gccgcctggg
     9181 tatagaatga ttccggtaaa tcggcgacca gtcgctgcca ggcttgtgcg cggcgttctg
     9241 gtggcgtcat tactgaatcc accccttgca aacggacatt acgcagaata aatggcatga
     9301 ccgtggttgg cagagtaaaa ccacccgcca gaccacaggc cgccacgcag ccgccgtaat
     9361 tcatttgcgc cagcactttt gccagcactt tgtcgccaac agtgtcaatt gccccagccc
     9421 agacttgttt ttccagagga cgggattcgg caaactcatc acgagggaga atacggctgg
     9481 cacctaaact tttcagatat tcatgggtac tttcgcgacc ggaaacggca acgacctgat
     9541 aacccaactt atgcagcagc gccacggcgg tactgccgac gccaccactg gcacccgtca
     9601 cgacaatctc cccgtcctgc gggcgaacac cggcatcttc cagcgccatc acacacagca
     9661 tggcggtaaa accggcagta ccgataatca ttgctttacg cgcgtccagc ccttgcggca
     9721 tggcaaccag ccagtcacct ttcactcgcg cctgctccgc cagcccaccc cagtggtttt
     9781 caccaacgcc ccagccagtg agtaacacct cctgaccggc atgaaaacgc ggatcttcgc
     9841 tggtgcgtac agttccggca aaatcgatcc caggaatcat cggaaaatta cggatgattt
     9901 ttcccttacc ggtaatcgcc agcgcatctt tatagttcag gctcgaccag tgaacatcga
     9961 ccgtgacatc gccctccggc aggcgacttt cgtccagagt ctgtactgat gcgagagttt
    10021 tgccgtcctg ctgttctaaa agtaacgcct gcataagtgg tcctcatgtg catgatggat
    10081 tggaaaataa tttctgaaga ctatactcgc taatggaaaa gcaaagccga tgaagcgcaa
    10141 taaattgcga gataaatctg atttgctagt atgcccgctt cctcactatc ggagttaaca
    10201 caaggatgag attaacgacg aaattttcgg cctttgttac gctgctcacc gggttaacaa
    10261 tttttgtgac tttgctgggc tgttcgctaa gtttctacaa cgccattcag tataagttta
    10321 gtcatcgcgt tcaggcggtg gcgacggcga ttgataccca ccttgtgtcg aatgacttca
    10381 gcgcattaag gccacaaatt accgaattaa tgatgtcggc agatatcgtt cgtgtagacc
    10441 tgctccatgg tgataagcag gtttataccc tggccagaaa tggtagttat cgtccggttg
    10501 gcaccagcga tctgtttcgt gaactgagcg ttccgttgat aaagcatccg gggatgtcgt
    10561 tgcgtctggt ttatcaggat ccgatgggca actatttcca ttcgttgatg accaccgcgc
    10621 cgctcacagg ggcgattggc tttatcattc ttatgctctt tctggcggta cgctggttac
    10681 aacggcaact tgccgggcaa gaattgctgg aaacccgggc tactcgtatc ttaaacggtg
    10741 agcgtggctc taatgtgttg ggaaccatct atgaatggcc gcccagaacc agcagtgcgc
    10801 tggatacgct gcttcgtgaa attcagaacg cacgcgaaca acacagccgt cttgatacgc
    10861 tgatccgctc ttatgccgca caggacgtga aaaccggcct caataaccga ctctttttcg
    10921 ataatcagtt agcaacgtta ctggaagatc aggagaaagt aggtacccac gggatcgtga
    10981 tgatgattcg tctgccggat ttcaatatgt tgagcgatac ctgggggcac agccaggttg
    11041 aagaacagtt cttcactctg acgaatctgc tgtcgacatt tatgatgcgc taccctggcg
    11101 cactgctggc gcgttaccac cgcagtgatt ttgctgcgct gttaccgcac cggacgttaa
    11161 aagaggcaga gagcatcgcc ggtcagttaa tcaaagccgt cgataccttg ccgaacaata
    11221 aaatgctcga tcgcgacgat atgatccaca ttggtatctg cgcctggcgt agtggtcagg
    11281 ataccgagca ggtaatggaa catgcagagt ctgccacgcg taatgcggga ttgcagggcg
    11341 gcaatagctg ggctatttac gatgactcgt tgcctgaaaa aggacgcggt aatgttcgct
    11401 ggcgtacgct tatcgagcaa atgctcagtc gcggcggccc gcgcctttat caaaaaccgg
    11461 cggttactcg cgaaggtcag gttcatcatc gcgaactcat gtgccgcatc tttgatggta
    11521 atgaagaggt tagctcggcg gagtatatgc cgatggtctt gcagtttggc ttatcggaag
    11581 agtatgaccg tctgcaaatc agccgtctta ttccactatt gcgttactgg ccagaggaaa
    11641 atctggcgat tcaggttacc gttgagtcgc tgattcgccc gcgttttcag cgttggctgc
    11701 gcgatacgtt aatgcaatgt gaaaaatcac aacgaaaacg cataattatt gaacttgcag
    11761 aggccgatgt aggtcaacat atcagtcgtt tacaacctgt tattcgttta gtgaatgctt
    11821 taggggtacg ggtagccgtc aaccaggctg gtttgacgct ggtaagcacc agttggatca
    11881 aagaacttaa tgttgagtta ctcaagctcc atccggggct ggtcagaaac attgagaagc
    11941 gaacggagaa ccagctgctg gttcaaagcc tggtggaagc ctgctcaggg accagcaccc
    12001 aggtttacgc caccggtgtg cgttcgcgaa gcgagtggca gaccctgatt cagcgcggtg
    12061 ttacaggcgg gcaaggggat tttttcgcgt cctcacagcc acttgatact aacgtgaaaa
    12121 aatattcaca aagatactcg gtttaacctg ccgtttaatc cgttttcacg tagaataacg
    12181 cgcgctgcgt ctcatgggag tgtgcttgtc tgctcgccag attgttgcag cacatatgca
    12241 gatgaatgac cttacgcggt tgcaaacagg cgaggaatgc tgctgatgca ttaagccttt
    12301 ctggactcag gcagagattt gtaacaaagg aaacgaactg cactaatttt caccgtagca
    12361 gatgattttt gcgccttgtc gctgctgcgt gtggttggta aagtaagcgg attttctttt
    12421 ccgccccagc tttcaggatt atcccttagt atgttgaaaa aatttcgtgg catgttttcc
    12481 aatgacttgt ccattgacct gggtactgcg aataccctca tttatgtaaa aggacaaggc
    12541 atcgtattga atgagccttc cgtggtggcc attcgtcagg atcgtgccgg ttcaccgaaa
    12601 agcgtagctg cagtaggtca tgacgcgaag cagatgctgg gccgtacgcc gggcaatatt
    12661 gctgccattc gcccaatgaa agacggcgtt atcgccgact tcttcgtgac tgaaaaaatg
    12721 ctccagcact tcatcaaaca agtgcacagc aacagcttta tgcgtccaag cccgcgcgtt
    12781 ctggtttgtg taccggttgg cgcgacccag gttgaacgcc gcgcaattcg tgaatccgcg
    12841 cagggcgctg gtgcccgtga agtcttcctg attgaagaac cgatggctgc cgcaattggt
    12901 gctggcctgc cggtttctga agcgaccggt tctatggtgg ttgatatcgg tggtggtacc
    12961 actgaagttg ctgttatctc cttgaacggc gtggtttact cctcttccgt acgcattggt
    13021 ggtgaccgtt tcgacgaagc tatcatcaac tatgtgcgtc gtaattacgg ttctctgatc
    13081 ggtgaagcca ccgcagaacg tatcaagcac gaaatcggtt cggcttatcc gggcgatgaa
    13141 gtccgtgaaa tcgaagttcg tggccgtaac ctggcagaag gtgttccacg cggttttacc
    13201 ctgaactcca acgaaatcct cgaagcactg caggaaccgc tgaccggtat tgtgagcgcg
    13261 gtaatggttg cactggaaca gtgcccgccg gaactggctt ccgacatctc cgagcgcggc
    13321 atggtactca ccggtggtgg cgcactgctg cgtaaccttg accgtttgtt aatggaagaa
    13381 accggcattc cagtcgttgt tgctgaagac ccgctgacct gtgtggcgcg gggtggcggc
    13441 aaagcgctgg aaatgatcga catgcacggc ggcgacctgt tcagcgaaga gtaatcggat
    13501 gcaggcaggg gaagcgtctg tttaccctgc ctggtctgat acgagaatac gcataactta
    13561 tgaagccaat ttttagccgt ggcccgtcgc tacagattcg ccttattctg gcggtgctgg
    13621 tggcgctcgg cattattatt gccgacagcc gcctggggac gttcagtcaa atccgtactt
    13681 atatggatac cgccgtcagt cctttctact ttgtttccaa tgctcctcgt gaattgctgg
    13741 atggcgtatc gcagacgctg gcctcgcgtg accaactaga acttgaaaat cgggcgttac
    13801 gtcaggaact gttgctgaaa aacagtgaac tgctgatgct tggacaatac aaacaggaga
    13861 acgcgcgtct gcgcgagctg ctgggttccc cgctgcgtca ggatgagcag aaaatggtga
    13921 ctcaggttat ctccacggtt aacgatcctt atagcgatca agttgttatc gataaaggta
    13981 gcgttaatgg cgtttatgaa ggccagccgg tcatcagcga caaaggtgtt gttggtcagg
    14041 tggtggccgt cgctaaactg accagtcgcg tgctgctgat ttgtgatgcg acccacgcgc
    14101 tgccaatcca ggtgctgcgc aacgatatcc gcgtaattgc agccggtaac ggttgtacgg
    14161 atgatttgca gcttgagcat ctgccagcga atacggatat tcgtgttggt gatgtgctgg
    14221 tgacctccgg tctgggcggt cgtttcccgg aaggctatcc ggtcgcggtt gtctcttccg
    14281 taaaactcga tacccagcgc gcttatactg tgattcaggc gcgtccgact gcagggctgc
    14341 aacgtttgcg ttatctgctg ctgctgtggg gggcagatcg taacggcgct aacccgatga
    14401 cgccggaaga ggtgcatcgt gttgctaatg aacgtctgat gcagatgatg ccgcaggtat
    14461 tgccttcgcc agacgcgatg gggccaaagt tacctgaacc ggcaacgggg atcgctcagc
    14521 cgactccgca gcaacccacg acaggaaatg cagctactgc gcctgctgcg cccacacagc
    14581 ccgctgctaa tcgctctcta caaagggcta cgccgccgca aagtggtgct caaccgcctg
    14641 cgcgtgcgcc gggagggcaa tagtggcgag ctatcgtagc cagggacgct gggtaatctg
    14701 gctctctttc ctcattgcgc ttttgctgca aatcatgccc tggccggata acctgattgt
    14761 tttccggcca aactgggtgt tactcatctt gttgtattgg atcctggcct tgccccatcg
    14821 cgtaaatgtg ggcacaggtt ttgtgatggg tgccatactg gatctgatca gcggctcgac
    14881 gcttggcgta cgcgtattgg cgatgagcat cattgcttac ctggtggcgc tgaaatacca
    14941 gcttttccgc aacctcgcat tatggcagca ggcgctggtc gtcatgttgc tttcgctggt
    15001 ggtggatatt attgttttct gggcagagtt tttagtgatt aacgtctctt tcagaccgga
    15061 agtgttctgg agtagtgtag tcaatggggt gctctggccg tggattttct tgctgatgcg
    15121 taaagtccgt cagcagtttg cagtgcaata aaggtttcta tgacttctct gtatttagct
    15181 tccggttctc cgcgtcgtca ggagttactt gcgcaacttg gcgtgacctt tgaacgtatt
    15241 gttacgggca ttgaggagca gcgtcagccg caggagagcg cgcagcagta tgttgtgcgt
    15301 ctggcgcgcg agaaagcacg ggcaggtgtc gcgcaaacgg cgaaggatct cccggtgctg
    15361 ggtgcggata ctatcgttat cctgaacgga gaagtgctgg agaaaccgcg cgacgcagag
    15421 catgcggcgc agatgttgcg caaattatcg ggtcagaccc atcaggtgat gacggcagtg
    15481 gcgttggccg acagccggca cattctcgat tgcctggtgg tcaccgatgt gactttcaga
    15541 acgttaacag acgaagacat cgcgggctat gtcgccagcg gtgaaccgtt agataaagca
    15601 ggtgcatacg gtattcaggg gctgggtggc tgttttgtca ggaagataaa tggcagctat
    15661 caagccgtag tcggcttacc gctggttgaa acgtatgaat tattaagtaa ttttaacgca
    15721 ctgcgtgaga aaagggataa acatgacggc tgaattgtta gtaaacgtaa cgccttcgga
    15781 aacgcgagtg gcgtatattg atggcggtat tctgcaggaa attcatattg aacgtgaggc
    15841 gcgacgcgga atagtaggca atatctacaa gggtcgtgta agtcgtgtac ttccgggtat
    15901 gcaggcggct tttgtagata ttgggctgga taaagccgcg tttcttcatg catctgacat
    15961 catgccgcac accgaatgtg tggcgggtga agaacaaaag caattcacgg tgcgcgacat
    16021 ctcggaactg gttcgtcagg ggcaagatct gatggtgcag gtggtgaaag atccgcttgg
    16081 cactaagggg gcgcgcctga ccaccgatat cacgctgcct tctcgctatc tggtctttat
    16141 gccaggggct tctcacgttg gggtttccca acgaattgaa agcgaatcag aacgtgaacg
    16201 cctgaaaaaa gtggtcgcag agtattgcga cgagcagggc gggtttatca tccgtaccgc
    16261 agcggaaggg gttggcgagg ctgaactggc ctccgatgcc gcttatctga aacgcgtctg
    16321 gaccaaagtt atggagcgta aaaagcgccc gcagacccgt tatcagctgt acggcgaact
    16381 ggcgttggcg cagcgtgttc tgcgtgattt cgccgatgcc gaactggacc gcattcgcgt
    16441 tgactcacgc ctgacttacg aagcgttact tgagttcacc tcggagtaca ttcccgagat
    16501 gaccagcaag ctggagcatt acaccggacg ccagccgatt ttcgatctct ttgatgtcga
    16561 aaacgaaatc cagcgagcgc tggaacgcaa agttgaactg aaatccggtg gttatctgat
    16621 tatcgaccag accgaagcga tgaccaccgt ggacatcaat accggagcgt ttgtcggtca
    16681 tcgcaatctg gacgacacca ttttcaatac caatattgaa gcgacgcagg ctatcgctcg
    16741 ccagttacgg ttgcgtaatc tgggcgggat tatcattatt gatttcatcg atatgaataa
    16801 tgaagatcac cgccgccgag tgctgcactc gctggagcag gcgttgagca aagaccgggt
    16861 gaaaaccagc gttaatggtt tttcggcgct ggggctggtg gagatgacgc gtaaacgcac
    16921 ccgcgaaagc attgagcacg tactgtgtaa cgaatgccca acctgccacg gtcgcggaac
    16981 ggtgaaaacc gtggaaacgg tatgctatga aatcatgcgc gagattgttc gtgtacacca
    17041 tgcttacgac tccgaccgtt tcctggtcta tgcttctccg gcagtagccg aagccttgaa
    17101 aggcgaagag tcacactcgc tggcggaagt ggaaattttc gttggcaaac aggttaaagt
    17161 acaaattgaa ccgctctata accaggagca gtttgacgtc gtaatgatgt aaacagatgc
    17221 tgggccgcca tccggcaaag ggtttttgag tcacattttt agcagacaag gagtgacggg
    17281 tgaggcgatt gccggggatt ttactgctta ctggagccgc gctcgttgtg atcgctgccc
    17341 tgctggttag cggcctgcgt attgctttac cgcatcttga cgcctggcgt ccggaaatcc
    17401 tcaacaaaat agaatccgcg actggcatgc cggtagaagc cagtcagctc tcagccagct
    17461 ggcagaattt tggcccgacg cttgaagcgc acgacatccg tgcagaacta aaggatggcg
    17521 gcgaattttc ggttaaacgc gttactctgg cgctggatgt ctggcagagc ctgttacata
    17581 tgcgctggca gtttcgcgac ctcactttct ggcagctgcg ctttcgcacc aacactccta
    17641 tcaccagcgg tggtagcgat gacagtctgg aagccagtca catcagcgat ctgtttcttc
    17701 gtcaatttga ccatttcgat ctccgcgaca gtgaagtcag tttcctgacg ccatccggtc
    17761 agcgcgccga gctggcgatc ccacaactca cctggctgaa cgatccacgt cgacaccgtg
    17821 cggaaggcct ggtaagcctc tccagcctta ccggacagca cggcgtgatg caggtgcgca
    17881 tggatttgcg cgatgatgag gggttgttaa gcaatggtcg cgtctggctc caggcggatg
    17941 acatcgacct gaagccgtgg ctcggtaaat ggatgcagga caatattgcg ctggaaacgg
    18001 cgcagttctc ccttgaaggc tggatgacga tcgacaaagg cgatgtaacc ggcggtgacg
    18061 tctggctgaa acagggtggt gccagctggt tgggcgagaa ggaaacgcat acgctgtcgg
    18121 tggataatct gaccgcgcat attacgcgtg aaaatccggg atggcagttc tctattcccg
    18181 atacacggat cacgatggac ggcaaaccct ggccgagcgg agcattgacg ctggcctgga
    18241 taccggaaca ggacgttggc ggcaaagaca ataaacgcag tgacgaactc cggattcgcg
    18301 ccagtaatct ggagctggca ggcctggagg gcatacgccc gctggccgcg aaactttcac
    18361 ctgcactggg tgatgtttgg cgctctacac aaccgagcgg caagattaac actctggcgc
    18421 tggatatccc gcttcaggcg gcagacaaga cccgttttca ggcatcgtgg agcgatctgg
    18481 cctggaagca atggaaatta ttaccgggtg cggaacactt ctccgggacg ctttccggca
    18541 gcgttgaaaa tggtttgctt accgcgtcga tgaagcaggc aaagatgcct tacgaaacgg
    18601 tattccgtgc gccactggaa atcgccgacg gccaggcaac tataagctgg ctgaacaatg
    18661 acaaaggttt ccagctggat gggcgtaata ttgacgttaa agccaaagcc gtccatgcgc
    18721 gcggcggttt tcgttacctg caacctgcta acgatgaacc ctggctgggt attctggctg
    18781 gcatcagtac cgatgatggt tcacaagcct ggcgctattt cccggaaaac ttgatgggta
    18841 aagacctggt tgattactta agtggcgcga ttcagggcgg tgaagcggat aacgcgacgc
    18901 tggtttatgg tggcaatccg caactcttcc cctataaaca caacgaaggt cagtttgaag
    18961 tgctggtgcc gctgcgcaac gcgaagtttg ccttccagcc ggactggcct gcattaacta
    19021 accttggtat tgaactggac tttattaacg acggtttatg gatgaaaacc gatggcgtta
    19081 atctgggcgg cgtgcgcgcg agtaatctca ccgcagtgat ccctgactac tcaaaagaaa
    19141 aactgctgat tgacgctgac attaaaggtc cgggtaaagc cgttggccct tactttgatg
    19201 agacaccgct gaaagattct ctgggtgcga ccctgcaaga actccagctc gacggcgatg
    19261 tgaatgctcg cttacatctt gatatcccgc tgaacggcga gctggtaacc gcgaaaggtg
    19321 aagtgacgct gcgtaataac agtctgttta tcaaaccact cgacagcacc ctgaaaaatt
    19381 tgagcggtaa attcagcttt atcaatggcg atctgcaaag tgaaccactg acagcaagct
    19441 ggtttaatca gccgttgaac gtggattttt ccaccaaaga aggggcaaaa gcctaccagg
    19501 tcgcggtgaa tctcaacggt aactggcaac cggcgaaaac cggcgttctg cctgcagcgg
    19561 tgaacgaagc attgagtggc agcgtggcgt gggatggtaa agtgggcatt gttctgcctt
    19621 atcatgctgg cgcgacgtat aacgtagagc taaacggcga tttgaagaat gtgagcagtc
    19681 acttaccttc accgttagcc aaacctgcgg gtgaaccact gccggtaaac gttaaggttg
    19741 atggcaatct caacagcttt gaattaaccg gacaggctgg tgcggataat catttcaata
    19801 gccgctggtt gctcggtcaa aagctgacgc tcgaccgtgc tatttgggcg gcagacagta
    19861 aaacgctccc gccgttgccg gaacaaagtg gtgttgaact caatatgccg ccgatgaatg
    19921 gtgccgagtg gctggccctg tttcagaaag gtgcggcgga gagtgtcggt ggtgcagcga
    19981 gtttcccaca acacataacg ttacgtacgc ctatgttgtc gctgggaaat cagcaatgga
    20041 ataacctgag tattgtttcg caaccgacgg caaatggcac ccaggttgag gcgcaggggc
    20101 gtgaaatcaa cgccacgctg gcgatgcgta ataacgcgcc gtggctggcg aatatcaaat
    20161 atctttatta caacccgagc gtggcgaaaa ctcgtggtga ttcaacgccg tcatcacctt
    20221 tcccgacaac ggaacgcatt aacttccgtg gctggtcgga cgcccaaata cgatgcgcag
    20281 agtgctggtt ctgggggcaa aaattcgggc gcattgacag tgatatcacc atttctggca
    20341 atacattaac gctgaccaat ggactgattg atactggttt ttcgcggctc actgccgatg
    20401 gtgaatggat taacaatccg gggaatgaac gtacctcgct gaaaggaaaa ctgcgcgggc
    20461 agaaaattga tgccgccgca gaattttttg gtgtcacgac gcccatacgg cagtcgtcat
    20521 ttaatgtgga ttacgattta cactggcgta aagcaccgtg gcagccagat gaggcgacgt
    20581 tgaatggcat cattcatact caactgggta aaggcgaaat taccgaaatc aataccggac
    20641 atgccgggca attgctgcgc ttattgagcg tagatgccct gatgcgtaag ctgcgttttg
    20701 atttcagaga cacttttggc gaagggttct attttgactc cattcgcagc accgcgtgga
    20761 ttaaagacgg cgttatgcac accgacgaca cgctggtgga tggcctggag gcggatatcg
    20821 ccatgaaagg gtcggtaaat ctggtacgtc gcgacctgaa tatggaagcg gttgtcgcac
    20881 cagagatttc tgcgacagtg ggcgtggctg cggcttttgc ggttaacccc attgttggcg
    20941 cggcagtgtt tgccgccagt aaagtgctgg ggccgctgtg gagcaaagtc tccattttgc
    21001 gctatcacat ttcgggtccg ctggacgatc cgcaaatcaa cgaagtgttg cgccaaccgc
    21061 gtaaagaaaa agcgcaatga tttgacgagg gcgcgtaatt gccccaatct cataggataa
    21121 tcgttgccaa aggccaacga gccagaacat aaccgtaggc tggatagggc gttcacgccg
    21181 catccggcag ccgtaaaaaa tcctctactg cagtaactaa cgagtagcaa aaacgatgag
    21241 tcttaacctg gtaagtgaac aattgctagc ggcgaacggc ctgaaacatc aggacttgtt
    21301 cgtgatcctc ggtcaactgg ccgaacgtcg ccttgattat ggcgatctct attttcagtc
    21361 gagctatcac gaatcctggg ttttagaaga ccgcattatt aaagatggtt cttacaacat
    21421 cgatcagggc gttggtgtgc gtgcaatcag cggtgaaaaa accggatttg cttacgctga
    21481 ccaaatcagc ctgctggcgc tggaacagag tgcgcaagcg gcgcgcacca tcgcccgtga
    21541 tagcggtgat ggcaaagtac agacgctggg cgcggtagag catagcccgt tgtatacctc
    21601 ggtagatccg ctgcaaagca tgagccgtga agagaagctg gatatcctgc gtcgcgtcga
    21661 taaggttgcc cgcgaagcgg acaagcgcgt acaggaagtg actgccagcc tcagtggtgt
    21721 ctatgaatta attctggttg cggccaccga cggcacgcta gcggcggatg tccgtccgct
    21781 ggtgcgtctt tccgtgagcg ttctgatcga agaagatggc aaacgcgaac gcggtgccag
    21841 tggcggcggc ggtcgttttg gttatgagtt cttccttgcc gatctcgacg gcgaagtccg
    21901 tgcggatgca tgggcaaaag aagcagtacg tatggcgctg gtcaatcttt ctgccgttgc
    21961 tgcaccagcg ggcaccatgc cggtagtact tggcgcaggt tggccgggcg tgctgttgca
    22021 tgaagcggta ggtcacggtc tggaaggcga cttcaaccgc cgtggcactt cagtatttag
    22081 tggacaggtc ggggagctgg tggcttcaga actgtgtacc gtggttgatg acggtacgat
    22141 ggtcgatcgc cgtggttcgg tggcgattga tgacgaaggt acgccaggcc agtacaacgt
    22201 gctgattgag aacggcattc tgaaaggcta catgcaggat aaactcaacg cgcgtttgat
    22261 ggggatgacg ccgactggca acggtcgccg tgaatcctac gcccatctgc ccatgccgcg
    22321 tatgaccaac acctatatgc tgccgggtaa atcgaccccg caggaaatta ttgaatccgt
    22381 tgagtacggt atctatgcac cgaactttgg tggcggtcag gtggatatca cctccggcaa
    22441 attcgttttc tccacttcag aagcatatct gattgaaaac ggtaaagtaa cgaagccggt
    22501 gaaaggcgca acgttgattg gttccggtat cgaaaccatg cagcagattt cgatggttgg
    22561 caacgacctg aaactggata acggcgtggg tgtctgcggt aaagaagggc aaagtttgcc
    22621 ggttggcgtg ggccagccaa cgctgaaagt tgataacctg actgttggcg gtactgcgta
    22681 ataatttcag tattttcaga aggataatta atccttcctc gtgcacgaac ggtcccctcg
    22741 cccctttggg gagagggtta gggtgagggg aacccgttgg cacaggtttg tacaacgtaa
    22801 cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa caatttaccg
    22861 acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag cggcagctta
    22921 tctttttcgg tatataacgc ataaaccggg cgtggatctg actgataacg cggcagcagg
    22981 atctccagct ccccacgatt gatctcgttg atcacccaca tcagcggcac gtaggcgatc
    23041 ccggcacccg ccgttagcca gcgcaccagc gtcatcggat cattagtcac aaatctccct
    23101 tgcgggatca ggcgagtcga gatcccttcc ggtgcaatca gttcaaattc attgtcgggc
    23161 cgcacgctgt attcaagcca tgaatggcta cttaaatcgg cgggtttttc cggtatgccg
    23221 tattgtgtga gatagctttt cgcggcgcac accaccattg gcatcgcgcc aagacggcgg
    23281 gaaaacaggc tggaatcctg caacgcgccg acgcggatca ccacatccag accgtcggca
    23341 atcaggtcgg gggctggaat tccggtaacc agattgacgc tcaaacttgg gtattctttc
    23401 agcattttgg ctgtcagccc ggcgagaaca ttttgtgcca tagttgaaga acagccaatg
    23461 cgtagcgtcc cgatgggggt gttattgaag gcatacagtt gctcatgaac atcctgcact
    23521 tcatgaagca tacgacggca gccctggtag taaattctac cggcttcggt caggccaatg
    23581 ctgcgtgtgc tacggtttaa cagctttacc tgcaactcat cttccagttt tgacaccgtc
    23641 tgactgatgg acgaaacgct catctgtagc tgtctggcgg cggcggtaaa agagccaaat
    23701 tcaactactt tggcaaacac cgacatgcgt tttagtcgtt ccattattca ctctgactta
    23761 aaagtgattt agatcacata atatagataa cagcataaca gttacgctaa tatattaaat
    23821 atcaatctac agcaatgttg ctctcgcccg gctttccatg cccttctctg cggcgacaga
    23881 tgctgaaaat aataacgcct gctctctcta tacaaccaag gtcaacatga gtctgtttcc
    23941 cgttatcgtg gtgtttgggc tgtccttccc accgatattt tttgaattgc ttttatcact
    24001 ggcgattttc tggctggtgt gccgggtact tgtgccaaca ggtatctacg actttgtctg
    24061 gcatccggcg ttgttcaaca ccgcgctcta ttgctgcttg ttttatttga tatcgcgact
    24121 gttcgtttga ggttgaagtg aaaatactaa taagaaaatt ctcccgtacg gccatcacgg
    24181 tcgtattagt cattctggcc ttcatcgcaa tttttaatgc ctgggtctat tacaccgaat
    24241 ccccctggac gcgtgacgcg cgctttagcg ctgacgtcgt tgcgatcgcg ccggacgttt
    24301 ctggactcat tacccaggtg aatgttcata accagctggt gaaaaaagga caggttctgt
    24361 tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga agcgcaagcc gatgttgctt
    24421 attatcaggt actggcacag gagaaacgcc aggaggccgg acgtcgtaac cgtctcggtg
    24481 tgcaggcgat gtctcgcgaa gagatcgacc aggccaacaa cgtactacaa acggttctgc
    24541 atcagttagc gaaagcgcag gcgacccgcg atctggcaaa actggatctt gaacgcacgg
    24601 tgatccgcgc gccagcagat ggctgggtga ccaacctcaa cgtctatacc ggtgagttta
    24661 ttactcgtgg atcaacggcg gttgcgctgg tgaaacagaa ctctttctat gtactggcct
    24721 atatggaaga aactaagctg gaaggggtgc gccctgggta tcgtgcagag atcacgccgc
    24781 ttggcagtaa caaagtgctg aaagggactg ttgatagtgt tgccgctggg gtcaccaacg
    24841 ccagcagcac gcgtgacgac aaagggatgg cgaccatcga ctctaacctt gaatgggtgc
    24901 gtttggcgca acgtgttccg gttcgtattc gtctcgacaa ccagcaagag aacatctggc
    24961 ctgcgggcac cactgctaca gtggtggtca ctggcaaaca agatcgcgac gaaagccagg
    25021 attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt tggttaatca cgatggatat
    25081 tttctccatt gctaaccaac atattcgctt tgcggtaaaa ctggcgaccg ccattgtact
    25141 ggcgctgttt gttggctttc acttccagct ggaaacgcca cgctgggcgg tactgacagc
    25201 ggcgattgtt gccgccggtc cggcctttgc tgcgggaggt gaaccgtatt ctggcgctat
    25261 tcgctatcgt ggctttttgc gcatcatcgg cacatttatt ggctgtattg ccggactggt
    25321 gatcatcatt gcgatgatcc gcgcaccatt attgatgatt ctggtgtgct gtatctgggc
    25381 cggtttttgt acctggatat cctcgctggt acgaatagaa aactcgtatg cgtgggggct
    25441 ggccggttat accgcgctga tcattgtgat caccattcag ccggaaccat tgcttacgcc
    25501 gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt attgtgtgtg cgattatggc
    25561 ggatttgctc ttttctccgc gatcgatcaa acaagaagtg gatcgagagc tggaaagttt
    25621 gctggtcgcg caatatcaat taatgcaact ctgtatcaag catggcgatg gtgaagttgt
    25681 cgataaagcc tggggcgacc tggtgcgacg caccacggcg ctacaaggca tgcgcagcaa
    25741 cctgaatatg gaatcttccc gctgggcgcg ggccaatcga cgtttaaaag cgatcaatac
    25801 gctatcgctg acgctgatta cccaatcctg cgaaacttat cttattcaga atacgcgccc
    25861 ggaattgatc actgatactt tccgcgaatt ttttgacacg ccggtagaaa ccgcgcagga
    25921 cgtccacaag cagctcaaac gcctgcgaag agttatcgcc tggaccgggg aacgggaaac
    25981 gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt tatcagcttc tcaagcgcgg
    26041 cgttatcagt aacacaaaaa tcaacgccac cgaagaagag atcctgcaag gcgaaccgga
    26101 agtcaaagta gagtcagccg aacgtcatca tgcgatggtt aacttctggc gaaccacact
    26161 ttcctgcatt ctgggaacgc ttttctggct gtggacgggc tggacttccg gcagtggtgc
    26221 aatggtgatg attgcggtcg tgacgtcact ggcaatgcgt ttgccgaatc cacgcatggt
    26281 ggcgatcgac tttatctacg ggacgctggc cgcgctgccg ttagggctgc tctacttttt
    26341 ggtgattatc cctaataccc aacagagcat gttgctgctg tgtattagcc tggcagtgct
    26401 gggattcttc ctcggaatag aagtacagaa acggcgactg ggctcgatgg gggcgctggc
    26461 cagcaccata aatattatcg tgctggataa cccgatgact ttccatttca gtcagtttct
    26521 cgacagcgca ttagggcaaa tcgtcggctg tgtgctcgcg ttcaccgtta ttttgctggt
    26581 gcgggataaa tcgcgcgaca ggactggacg tgtactgctt aatcagtttg tttctgccgc
    26641 tgtttccgcg atgactacca atgtggcacg tcgtaaagag aaccacctcc cggcacttta
    26701 tcagcagctg tttttgctga tgaataagtt cccaggggat ttgccgaaat ttcgcctggc
    26761 gctgacgatg attatcgccc accagcgcct gcgtgatgcg ccgatcccgg ttaacgagga
    26821 tttatcggcg tttcaccgac aaatgcgccg cacagcagac catgtgatat ctgcccgtag
    26881 cgatgataaa cgtcgtcggt actttggtca gttgctggaa gaactggaaa tctaccagga
    26941 aaagctatgc atctggcaag cgccaccgca ggtgacggaa ccggttcatc ggctggcggg
    27001 gatgctccat aagtatcaac atgcgttgac cgatagttaa gtcaaaaccg acgccaaaag
    27061 cgtcggtttt ttcatggcta tacttagcga tgcacggcag aactcgccgc gaaacgtgac
    27121 ggtggcaaca gatgaatatt tatacctttg attttgatga gattgagagt caggaggatt
    27181 tttatcgtga ctttagccaa acctttggtc tggcgaaaga taaggtacgc gatctcgact
    27241 cactatggga tgtgttaatg aacgatgtcc tgccgctacc acttgagatt gaatttgttc
    27301 atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat attgctgttt gatgaggcag
    27361 aggaagagct ggaagggcat ttgcgtttta atgttcgtca ttagcgcaaa aaaaagcccc
    27421 cgaaccgggg gcaatatcgt cggacaagac gatgagggtt tatttgtaca gttcagccgt
    27481 agcgtgccag gtgtcaccgc tacgagcttc agtaatctgg taggccgttg cgcctttctc
    27541 ttccgctttt ttgttcagca tttcacgcat atccattggc gaagacgcca caccacttac
    27601 ggatacggtc ccgattgctt cacgattttg tgcttgtgca gcatcaatgg agtcggcagc
    27661 gaatgcaccg aaagagagaa cagaaagtac gcttaatgca gcaacagtgg ttttgatttt
    27721 catgattttt acctcgacat aatcttttag ctgggccttt gtttcgtgac ccatatcaca
    27781 aaatcaagta tacactaatt attgagctaa ttaataccgc gctaactgtt tcagttgata
    27841 ctaagtgtta aaaatgtgaa ttttaataac aaaaaagaat taaaaatacg gttatttatc
    27901 gtta
//
LOCUS       DAZFBR010000064        27646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFBR010000064 DAZFBR010000000
VERSION     DAZFBR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.064"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..1255)
                     /gene="espK"
                     /locus_tag="ACHJZ2_002879"
     CDS             complement(<1..1255)
                     /gene="espK"
                     /locus_tag="ACHJZ2_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO4568956.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNNQIMKNI"
     gene            complement(1619..2482)
                     /gene="potB"
                     /locus_tag="ACHJZ2_002880"
     CDS             complement(1619..2482)
                     /gene="potB"
                     /locus_tag="ACHJZ2_002880"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO4568957.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(2466..3602)
                     /gene="potA"
                     /locus_tag="ACHJZ2_002881"
     CDS             complement(2466..3602)
                     /gene="potA"
                     /locus_tag="ACHJZ2_002881"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO4568958.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            3852..5078
                     /gene="pepT"
                     /locus_tag="ACHJZ2_002882"
     CDS             3852..5078
                     /gene="pepT"
                     /locus_tag="ACHJZ2_002882"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO4568959.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(5127..6248)
                     /gene="roxA"
                     /locus_tag="ACHJZ2_002883"
                     /gene_synonym="ycfD"
     CDS             complement(5127..6248)
                     /gene="roxA"
                     /locus_tag="ACHJZ2_002883"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO4568960.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            6497..7726
                     /locus_tag="ACHJZ2_002884"
                     /pseudo
     CDS             6497..7726
                     /locus_tag="ACHJZ2_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            8091..8279
                     /locus_tag="ACHJZ2_002885"
     CDS             8091..8279
                     /locus_tag="ACHJZ2_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4568961.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            8337..8891
                     /locus_tag="ACHJZ2_002886"
                     /pseudo
     CDS             8337..8891
                     /locus_tag="ACHJZ2_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            9084..9281
                     /locus_tag="ACHJZ2_002887"
     CDS             9084..9281
                     /locus_tag="ACHJZ2_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4568962.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            9274..9486
                     /locus_tag="ACHJZ2_002888"
     CDS             9274..9486
                     /locus_tag="ACHJZ2_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568963.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            9476..9940
                     /locus_tag="ACHJZ2_002889"
     CDS             9476..9940
                     /locus_tag="ACHJZ2_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568964.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            9933..10166
                     /locus_tag="ACHJZ2_002890"
     CDS             9933..10166
                     /locus_tag="ACHJZ2_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568965.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            10172..10471
                     /locus_tag="ACHJZ2_002891"
     CDS             10172..10471
                     /locus_tag="ACHJZ2_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4568966.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            10468..11868
                     /locus_tag="ACHJZ2_002892"
     CDS             10468..11868
                     /locus_tag="ACHJZ2_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO4568967.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELAELRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITLLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            12069..12320
                     /locus_tag="ACHJZ2_002893"
     CDS             12069..12320
                     /locus_tag="ACHJZ2_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568968.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            12317..12727
                     /locus_tag="ACHJZ2_002894"
     CDS             12317..12727
                     /locus_tag="ACHJZ2_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4568969.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            12738..13010
                     /locus_tag="ACHJZ2_002895"
     CDS             12738..13010
                     /locus_tag="ACHJZ2_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4568970.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            12967..13095
                     /locus_tag="ACHJZ2_002896"
     CDS             12967..13095
                     /locus_tag="ACHJZ2_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO4568971.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            13137..13361
                     /locus_tag="ACHJZ2_002897"
     CDS             13137..13361
                     /locus_tag="ACHJZ2_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568972.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            13613..13819
                     /locus_tag="ACHJZ2_002898"
     CDS             13613..13819
                     /locus_tag="ACHJZ2_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568973.1"
                     /translation="MAEEKKSGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            13819..14874
                     /locus_tag="ACHJZ2_002899"
     CDS             13819..14874
                     /locus_tag="ACHJZ2_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4568974.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            14887..15222
                     /locus_tag="ACHJZ2_002900"
     CDS             14887..15222
                     /locus_tag="ACHJZ2_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4568975.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            15235..15648
                     /locus_tag="ACHJZ2_002901"
     CDS             15235..15648
                     /locus_tag="ACHJZ2_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFO4568976.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPCADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            15854..16396
                     /locus_tag="ACHJZ2_002902"
     CDS             15854..16396
                     /locus_tag="ACHJZ2_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4568977.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            16652..16933
                     /locus_tag="ACHJZ2_002903"
     CDS             16652..16933
                     /locus_tag="ACHJZ2_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568978.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(17534..18994)
                     /gene="phoQ"
                     /locus_tag="ACHJZ2_002904"
     CDS             complement(17534..18994)
                     /gene="phoQ"
                     /locus_tag="ACHJZ2_002904"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO4568979.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(18994..19665)
                     /gene="phoP"
                     /locus_tag="ACHJZ2_002905"
     CDS             complement(18994..19665)
                     /gene="phoP"
                     /locus_tag="ACHJZ2_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO4568980.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(19834..21204)
                     /gene="purB"
                     /locus_tag="ACHJZ2_002906"
     CDS             complement(19834..21204)
                     /gene="purB"
                     /locus_tag="ACHJZ2_002906"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO4568981.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(21208..21849)
                     /gene="hflD"
                     /locus_tag="ACHJZ2_002907"
     CDS             complement(21208..21849)
                     /gene="hflD"
                     /locus_tag="ACHJZ2_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO4568982.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(21885..22991)
                     /gene="mnmA"
                     /locus_tag="ACHJZ2_002908"
                     /gene_synonym="trmU"
     CDS             complement(21885..22991)
                     /gene="mnmA"
                     /locus_tag="ACHJZ2_002908"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO4568983.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            complement(23045..23506)
                     /gene="nudJ"
                     /locus_tag="ACHJZ2_002909"
     CDS             complement(23045..23506)
                     /gene="nudJ"
                     /locus_tag="ACHJZ2_002909"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO4568984.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(23516..24169)
                     /gene="rluE"
                     /locus_tag="ACHJZ2_002910"
     CDS             complement(23516..24169)
                     /gene="rluE"
                     /locus_tag="ACHJZ2_002910"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO4568985.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            24341..25591
                     /gene="icd"
                     /locus_tag="ACHJZ2_002911"
     CDS             24341..25591
                     /gene="icd"
                     /locus_tag="ACHJZ2_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO4568986.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(25705..26847)
                     /locus_tag="ACHJZ2_002912"
     CDS             complement(25705..26847)
                     /locus_tag="ACHJZ2_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4568987.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(26837..27073)
                     /locus_tag="ACHJZ2_002913"
     CDS             complement(26837..27073)
                     /locus_tag="ACHJZ2_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4568988.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            27213..27646
                     /locus_tag="ACHJZ2_002914"
                     /pseudo
     CDS             27213..27646
                     /locus_tag="ACHJZ2_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT         7271 a         6476 c         7015 g         6884 t
ORIGIN      
        1 ttatgttttt cattatttgg ttgttgtctt tattacggta aatccatcgt aattcgctgg
       61 cggtaatact ctggccatag ccctctgact tgttagctat gagtttgagt gagttgacaa
      121 gctcatccgc acaaaaaata accgttttat tgcgtgttaa tgtctgaaac tctaggccgg
      181 ccttgcttgt ttttgcccat aatttgtcgc gattgtatac ggaaaggtcg gccatggtaa
      241 cattataatt attgtgattc tttaagtgtt gtttaaattt tattcctctt tcgtgttcat
      301 ttaatgctct tttgataagt ggatcttgct tatatttgcg aggtgttctg gggtcccata
      361 ctgcgtttgt taacgtattc tgagttataa tatctttttt ggaaaagtcg ttaactagtt
      421 taatactgtt gacaagtttt tctctgggtg aagatagtcc atagacaatg tctccctcgc
      481 gaaagtgtaa ggccacatcc agagcgttgc cctgaaaggt gatgtttttt gctttaacta
      541 catctggaga gagtagtaca ttttgtaaat caagatccag cccatcaggc atcccttcaa
      601 caccgatgtt ccacgtggtt ttctgctccg catgaatggt taccgatgtc acactctccg
      661 gaagtttctc cggcagtata atattgctac atccagtcag cgatagcgtc ttcagtgacg
      721 gtgatatcag gttgtcaatt ctggcctgat tctccgggtt atagctattg atgctgagag
      781 atgataaccc gtttggaaca ttttttatgc tgtctgtggc gctacctttt atttcaaggt
      841 agcggacact ctctggcact ccagatatat agcagtggca aaccgtcagg cactcgatgc
      901 cttcagatat gaaatgtata aatggtgaat cagttaattc aatatgtaga ttttttacgg
      961 ttgatggcat gtctgtgatt tgtaattttt cacagttaat gacggagagg ttttgtgtat
     1021 taaccggcaa tcctgtaagg gttgtcaggt tgttgcagtt ttcgattgta actgatgtta
     1081 tttcgtttgg taactgtggc agatactcaa tatcacaatc tttgatatat aaacgaccac
     1141 tggcccgcgc ttcttctatc tgaattggtg tgatttcgct tttgatgtct gctgatgttt
     1201 cagcggaata ggagcagaat gtcccggtcg gtcgtaattg cgatgtagga agcattgata
     1261 aattccattt ttagaaataa ataacagagt aagttctatc catactacta atcaacaagc
     1321 taattttaac aaccagcaaa gccaaggtgt caatattaga aaagagagtt atatctcaaa
     1381 accaacagac tgttgatttt aacttttgcg aatactttct ccagtccgtc atgcatggat
     1441 taggattgct catttttata ccatttgttg tttttttata gcgatttgct aaaagggaga
     1501 agaaaaaacg ttctccaaaa ttagattgca actgtttgat tttatggata gaggcatgct
     1561 gtttactaga cagtaaaaag catgccaaat ttcctattaa ccttttgaat ataaagcact
     1621 aattatctat ttctgttgcc ttcttattca gcagacgaga agcgcgccag taaaccagca
     1681 acatcaggcc cattacgata gtcagcgtaa tgctggtggc tgcaccaaac ggccagtcac
     1741 gaatattgag gaactgtacc ttgatgacgt taccgatcag caggtttttc gcaccgccca
     1801 tcaggtcgga cacatagaac aggcccatcg ctggcagcat caccagcaga catccggcaa
     1861 taatccccgg catcgtcagt ggaataatga tgcggataaa agtctgtaac ttgctggcac
     1921 cgagatcgcg cgccgcctca agcaacggct tatccagttt ttcgatactg gagtacagcg
     1981 gcatcaccat aaacggtagc agaatgtaaa ccagaccgat aatcaccgca ctgggcgtga
     2041 acatgatacg aattggtgtg tcgataacgc ccagccagag caaaaactcg ttgagatagc
     2101 ctttggtgct gaggaaaatt ttcagcccgt agatgcgaat cagtgagttg gtccagaagg
     2161 gaacaatcag cagaaacagc agcagcggac gcaccttgtg tggcagcttc gccagaaacc
     2221 aggcaaacgg atagcccagc accaggcagg cgagggtggc gatcagcgcc atattcagcg
     2281 agtgcaatag cacttcaaaa tagagcggat cgagcagacg tgtgtagtta tccagcgtaa
     2341 agaccatttt gacaaaactg gcgtcgtcgc gggccaaaaa gctggtgcca atgatcatca
     2401 ggttgggcag aaagacaaac aacacaagcc aaccgacaat agtgacaatc actacattct
     2461 ggaacttact tgtgttcttc atcagccagt acgacctccc agctttctac ccaattaatg
     2521 gccatttttt ggtcgagaga gtggtcaaag tcaggatcgt cttcattgaa gaattcgctg
     2581 accatcacca tcttgccatt ttccagttca acaaccgact ccagcgtcat gcctttgtag
     2641 ttacgctcgc gaacgtaacc aatcagccct tcagcgtggt tgtcgtcgtt aatctcttca
     2701 acacgtaagt cttccgggcg cagcagaaca tgcagttttt gccccggttc aacggcgaag
     2761 ttaacgtaga tattacattc gcggccttca acgttggcgc gtacgcgctg ctcgtcgaga
     2821 cgttcgatga cagtggcgtt aaacatattg atttcgccaa tgaacccggc aacaaacagg
     2881 tttttcggct cttcgtagat ttcacgcggc gtgccgtctt gctcaatgcg accatcgcgc
     2941 atcaccacaa tcctgtctga catggtgagt gcttcttcct gatcgtgagt cacgaagacg
     3001 aatgtaatgc caagcttacg ctgtaacgct ttcagctcgt tctgcatttg cttacgcagt
     3061 ttgtaatcca gcgctgagag cgactcatcc agcaacaaca gacgaggctt gttaaccacc
     3121 gcgcgagcaa tggcgacgcg ttgctgttga ccaccagaga gctgatgcgg tttacgttga
     3181 gcgaaggttt ccagctgcac catccgcagg gcttccatca cgcggggcgt aatttcagca
     3241 gcgggggttt tttgcatgcg caacccaaag gccacatttt cgaacacggt catgtggggg
     3301 aaaagtgcgt agctttggaa aacagtgttc acatagcggt tttccgccgg aacgtgggtg
     3361 atgtcctcgt tatccaacat gatgcgtccg gaatcaacag tttccagacc tgcaatcagg
     3421 cgaagaacgg ttgttttacc gcagccagaa gggccaagca gcgtgaggaa ctcgccattg
     3481 ttgatagtca gatccagctg gggaatgacc tctttaccat caaagcattt gcgaattccc
     3541 gccaattgca ccagcggtga aagcgaactc ggttgtttat tcaatttttt actctgtccc
     3601 atgtaaacgc aacggatggc ttaccgatgc ggggtttgtg gttaaccacc ttggtgactc
     3661 ttaatgaggg cggtaattct acggcaaacc gcttgaatcg ccaatctttg ttgtgaatta
     3721 ctggcttagc tttatattca ttaaggtaat gctgataaat attcccgctt gcaggggtaa
     3781 aagtgacctg acgcaatatt tgtcttttct tgcttcttaa taatgttgtc acaaaaagtg
     3841 agggtgacta catggataaa ctacttgagc gatttttgaa ctacgtgtct ctggataccc
     3901 aatcaaaagc aggggtgaga caggttccca gcacggaagg ccaatggaag ttattgcatc
     3961 tgctgaaaga gcagctcgaa gagatggggc ttatcaatgt gaccttaagt gagaagggca
     4021 ctttgatggc gacgttaccg gctaacgtcc ctggcgatat cccggcgatt ggctttattt
     4081 ctcatgtgga tacctcaccg gattgcagcg gcaaaaatgt gaatccgcaa attgttgaaa
     4141 actatcgcgg tggcgatatt gcgctgggta tcggcgatga agttttatca ccggttatgt
     4201 tcccggtgct gcatcagcta ctgggtcaga cgctgattac caccgatggt aaaaccttgt
     4261 taggtgccga tgacaaagca ggtattgcag aaatcatgac cgcgctggcg gtattgcaac
     4321 agaaaaacat tccgcatggt gatattcgcg tcgcctttac cccggatgaa gaagtgggca
     4381 aaggggcgaa acattttgat gttgatgcct tcgatgcccg ctgggcttac actgttgatg
     4441 gtggtggcgt aggcgaactg gagtttgaaa acttcaacgc cgcatcggtc aatatcaaaa
     4501 ttgtcggtaa caatgttcat ccgggcacgg cgaaaggagt gatggtaaat gcgctgtcgc
     4561 tggcggcacg tattcatgcg gaagttccgg cggatgaaag cccggaaatg acagaaggct
     4621 atgaaggttt ctatcacctg gcgagcatga aaggcaccgt tgaacgggcc gatatgcact
     4681 acatcatccg tgatttcgac cgtaaacagt ttgaagcgcg taaacgtaaa atgatggaga
     4741 tcgccaaaaa agtgggcaaa gggttacatc ctgattgcta cattgaattg gtgattgaag
     4801 acagttacta caatatgcgc gagaaagtgg ttgagcatcc gcatattctc gatatcgccc
     4861 agcaggcgat gcgtgactgc gatattgaac cggaactgaa accgatccgc ggcggtaccg
     4921 acggcgcgca gttgtcgttt atgggattac cgtgcccgaa cctgttcact ggcggttaca
     4981 actatcatgg taagcatgag tttgtgactc tggaaggtat ggaaaaagcg gtgcaggtga
     5041 tcgtccgtat tgccgagtta acggcgcaac ggaagtaagc gaaaagggat gcggcatgtg
     5101 atgccgcatc cggcttaaat ccaaacttac ccttcgaaga accaataccc gctattgacc
     5161 agcgccgcga gcatcgcgag gaatgacgga tcttccagcg catcgccaaa attctccgca
     5221 gtcagcgcaa tgttgctggc gagtgcatcc agtgccggac ggtgcgggga atcgatcttc
     5281 tcaccattgg tatacacgtc gtcgccaatg cgcaatacgc gcagaccacc caggcgcacc
     5341 agcacatcac cttgtttcag cgcatcgtag atttcatctg gctgataagg cggctccggc
     5401 ggcgcgatat ccagttcatg acgtgactgg gatataaact cgccaaacca ttgcttaaag
     5461 tgttccggct ggttgatcaa ttcgagcatc atctcacgca gtttatccat ctcttgcggc
     5521 agaacgtccg caggatgagc gcgaggtgga acatcaggat cgctgtagta gttgccgccc
     5581 agttcgcgtt gcagcacata atcggcaaat ccgctaatta attcccgcgt atttggcgcg
     5641 cgaaaaccca cggaatagtt catcgcattt tccagcgcgt agccttcatg cgggaatcct
     5701 ggcggaatat aaagaatatc gccaggctcc agctcttcat cgatgatggc ttcgaacgga
     5761 tcgacctgta acagatccgg atgtgggcag tgctgtttca tttgcagctt ctcgcccact
     5821 cgccagcgac gacgtccggt accctgaatg ataaacacgt cgtactgatc gagatgcggg
     5881 ccgacgccgc cgccaggtac agaaaaagag atcatcagat catcaatacg ccagtccggt
     5941 agttcacgga acggtcgcat cagcgcggcg gtcggctcat gccagtggtt cactgcttgc
     6001 actaacaatg accagttagt ttcaccgaga tgatcgtagc tttcgaacgg accgtggctg
     6061 acctgccatt ttccatcctg gtgactgacc agtcgactgt cgacttcgct ttccatcgcc
     6121 agacccgcca actcgtctgg agagatcggg tcaataaaat tattaaagcc gcgttttaac
     6181 accaccgggc gtttctgcca gtgacgttca agaaaatcgg gccagttaag agtgagttgg
     6241 tattccatgt taagcatcca caggctggta tctgcaaccg attataacgg atgcttaacg
     6301 taatgcgtga agtatggaca tattttatca attagtgttt tttgccgttt cataatgttt
     6361 cagattgttc attatatttt tgattataaa cagatttgtg atttttcttg ttccagcttg
     6421 tttcacgttg tagccctttg tgcaacgatc ggtgtagtta atggtgtagt taattgtgat
     6481 gaccggaatc aaaattatga gcagagcact taacaaactg agcgatacac agctgaggaa
     6541 aatcaacggc acacccgccc aaaaaacagc ctttcttaat gacggtggaa acctgagcgt
     6601 caggcattca accagtggcc ttttaacctg gtatttcact tacagggccg gaacgggaag
     6661 gggggcacca ccggaacgca ttaagctggg aaattatcct gatctgagcc tgaaataagc
     6721 cagggaaaaa gccgcccagt gtcgcgcatg gctggcagag gggaaaaatc cacgtcatga
     6781 gcttaattac accgtacagg aagcgttaaa gccggtaacg gttggcgatg cgctcaccta
     6841 ctggcttgag tcgtacgcaa aggaaaaccg cgtggattat gccgccctga aaaagcgcct
     6901 taataatcac gtaatacagc acattggtgc tatgccgctg gataaatgcg agctacggca
     6961 ctggctggcc tgttttgacc aggtggcaaa gcgaacgcct gttactgccg gattcttgct
     7021 acagacgtgc aaacaggcgc ttaagttctg ccggaggcgg cgctatgcaa tcagcaacgt
     7081 tcttgatgat atgagtgtgg cggacgttgg gaaaaaaccg gatataagcg agcgtgtctt
     7141 aagcaccaaa gaactgggcg aattattgca ggcactggac aaaaaaatat tctcccccta
     7201 ctacatcgcg ttaatccgcc tcctgattgt gttcggatgc cggacggtag aactgaggtt
     7261 atcggagatc agcgagtggg attttaccga aatgctctgg accgttccga aggagcacag
     7321 caaaacgaag gtagccatat tccggcctat accggaagca atactgccgt tcatcacgca
     7381 gctggtggag cagaacaggc acacgggctt attgctgggg gaagtgaaac aggaggccag
     7441 cgtatcgcag tatggaagat tagcgcacag gaggcttaat caccctcact ggtcactgca
     7501 tgacatccgg cgcaccttta caaccatgct gaacgattta ggcgttgatc cgcatgtcgt
     7561 ggagcagctt acaggccacc agatgccagg aatgcagcga gtttataatc attcccgtta
     7621 tctggatgcg aaacgcaatg cgctggatat gtggacggag cggttaggga tactggcggg
     7681 aacacatgaa aacgtaacca cgctaccagt agccagaaga aaataatttt tttcgtgttt
     7741 tttcagtatg cgcatactgg atatgcgaac agatacaacg tgcaacaatg agcaacaatg
     7801 tggaacaact acgaacaaga ggcgaaaaag cgtacggatt tataagtagc tgatttttaa
     7861 tgtgttactg gttttttata cactagcgaa tcactcttta aagagcgaga aaaaaggcat
     7921 ggaacaatat tttatgcctt tgtattaaca tgaatttaaa agattttatt cttacttttt
     7981 cattttctgt aaatcataca tcctcagtat ttgccattca tcactctagc gggaacaata
     8041 cgacacaata agacacctga tgactcttta agaaacgaaa gggggcaata gtgttaagca
     8101 ctgatcggtt tatacgtgaa aaagaatgcg aaaaactaac cggccttagc cgtacgtgtc
     8161 gctaccgcct ggaaaaggcc ggacaattcc catcacgtcg taaacttggc ggtcgttccg
     8221 ttggctggtc tttatccgag gttctggcct ggaaggatag ctgcaaggca gttcattaat
     8281 cacgctggcg gcacacagcc gccacacatc aatcatctga acacagagct ataaccatga
     8341 agattgaata tacgccagaa cgtgggcggg gattcgttcg ccctggtgag actggaaaac
     8401 cacaaaattg gggtttttca ggcataaaaa aagcggcccc gaaatggagc cgcctttctg
     8461 aacagataac ccgctgcgcc ttatgtgtat atgatcccaa acataagcac ggggatgata
     8521 gccgctatca ggctggtggg caatgctatc agtctggttc agttcgttgc catacctgca
     8581 atgggcgcgt ttccctgtac tctttaagga attgctcaag ggcaaaagca catggcgcga
     8641 atctttctga ttcatgctct atctttctgc gccgtctttt ccgtgccggt gataatgttt
     8701 tggtcaattc tttatcggtc attgtgttgt cctgcatagc aatgcgccgt aatacctcac
     8761 accacggcgc tgatagtttt tatcctttgg gttctatgcc gcgttgctgt aattctttgc
     8821 ggagaacgcg tttaatccaa gtggctaaag atgtgtcgcc atcggcctta gcagcttctt
     8881 ctagctgcgc tctgaagtct tccggtaatc gcatttggta tggaggcgaa cgtttttcta
     8941 atggtgttga catggtaatt accctttatt gctataccta catggtaatt accattgtac
     9001 ttgccattac acaaaaatca acgccccgca gtgctgggaa cacatacggg gcgtctaacc
     9061 aacaacgtaa actaggagcc gttatggttg ccgtaaatca tataccacac cttgtacaca
     9121 cacaaacggc ctttgtgtgg cgttttctgg cactgagtgc cggagaatct caaatcatcc
     9181 acgtaaccgc ctggacggaa cgcgaagcgc gtaaccgttg cccgtccggt tgtgttgctg
     9241 tatttgccgc ccgtattcgt caggaggtgc gccatgatta atctgtcact tactgacctt
     9301 aaccgcattc agtttcgtga gaaattcaca gggcagctac tggtcaatgt ggataaaggt
     9361 cgcgtggtgt gtaattacca cttaccagat gaagcaattg tcgccacaag ggaatcatta
     9421 caggaaatca caaaacgtgc cggaatgatt aacacgaata ttcaggggat gctttatgca
     9481 caataaaacc acaccggacg cagccgccga agccatcaaa acgctgatgc acgcgctgat
     9541 tgatatttct gttatcgcgg acagggcaca taagcacgcc accagcgaaa cagaatatgc
     9601 cggtgctttc gttcctcact cactggctgt catgcaattt agtgctgata cggcgctgaa
     9661 tgaggccaaa gctatcctga ttgctgattg tgaaaatggg ggggttatgc gtgatgatcg
     9721 ttttaattcc ctgaaacagg aattttccgg cgttcctgat gatgcggctg atgcgctttc
     9781 gtcaatgcca gaacttatta gagcggcttt tttcttactt tccacgagag aatataaatc
     9841 aacggggctt gatgtactga atatcgccgc cgattatgcg gaatatgtgg cagaggcgcg
     9901 ttatagaaga aaatttcctg aggatgtaag ccatgcgtga tatttaccac caaacaatag
     9961 accgcgcatt tcttgcactt tctcacagtg aaaacatgat ggaaatattg cgcatatggc
    10021 ttgaaacact tggcgacaat gaacgcgaca aacaaaaatc aagaattgcc acggcattaa
    10081 taacgcttct tgagcctgta ataatggaac tgcaagaaat agatctattg cacgacagat
    10141 ataaagaaca gcacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc
    10201 cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg
    10261 cacggtaatg attaagggcg tggaagaacg gcgcgtaacc taccgccgtg aaggttacga
    10321 atatgattgc gtgatgcctg tttatcagtt ccggcgtgat ttttctctgg tacagaccgc
    10381 gccgcataac gtgcccacca gcaacgccag ggcacgggca aacatccaga agctgaaaac
    10441 catggttaac ggattcaggg gcaagaaatg aaaagcgcac cgaacttaaa aaaacagcct
    10501 tacgacaaga tgaccgaagt cattattttt gcgggtagtg atgcctgggc acatgcgaaa
    10561 cagtggcagg aacaggacgg gcgactggct ggcgataatg tgcctcccgt tgtgctggct
    10621 gatgatcaac tggatgaact ggcagaactg agaatcatcg acgaggggcg ctattgtgtc
    10681 cggctgtaca aggcaggcca catcaggcca tcaaatatta atgccattgc gcacaagctg
    10741 gcggcggcgg gtgtaactga tgcgaattat taccccgaag ggatgcacag ccatatgcgg
    10801 gagaactggc gcgaatacct ggaacgggtg cgcgggaaag agccggcgga agaaaaaaac
    10861 caccagcgaa aaaccacgct accgatgagc gttggatcta ccggatacga cacgcaactg
    10921 gattacgtgg ttaaggggat tattccggcg gtatcgctat gcagcatata cggggctagc
    10981 gggtcctata aatcattcct tgccggatcg tgggcgtgcc atgttgccac tggtcgccag
    11041 tggggaggcc gcagggttgc acatggtgcg gttctctatg tggttggtga aggcggtata
    11101 ggtgttccgc gtcgtgtaaa agcctgggag gttgtgcacg atgagcaggt gaaaaatctg
    11161 tatctggtaa accgccccat ctttccggct gccccgcttg atgttgatga aatggttatc
    11221 gctgcccgtc aggtggagcg ggaaacgggt aaacctgtac gcatgattat tctggatacg
    11281 ctggcgcgtt gctttggtgg gaatgatgaa aatgattccc gtgatatggg ggcgtttatc
    11341 cgtggttgtg acgaactgaa acgacgcaca ggggccacgg tgctggtggt tcaccattcc
    11401 ggcaaggatg agacgaaagg cgcgcgcggt tccagtgcat ttcgtgcttc gctggatgct
    11461 gaataccgga tacgcaggga ggacgcagga agcgaagcgc tggttatctc atgcaccaaa
    11521 atgaaggacg cggaggaact caaagaagcc gcatatgact tacgcgtggt ggagcttttt
    11581 accgacgctg acggtgaatt aatcacgttg ctggtggtgg tggatgatcc gcgccctcct
    11641 gttgaactgg agcgcatcga ggaggcaggg aacaagacgg aaaaccatac cgcgctatgg
    11701 gggtgcatcc gttcacgcac acagaacggc gacaagtgca cgatcccgct gttacgtgat
    11761 gacatgaaaa agctggggta tgaaatgaaa aacttccggc gctggctgta caagctggaa
    11821 aaagatgggg ttattcgtat cgatggggat gatgtagcgc cgctataaaa gtgaggagca
    11881 aaagcgaggg ggatagaaag agggccaaaa ttagcccgct ctccctcact tttcgacctg
    11941 tatacatcct caaaagtgag gggtaaaaaa atacttatga aacacacaca tagaaaaacc
    12001 gaaaatccca actgcgacga agtgagacgc ttgaaaaagt gaggcgaaaa agtgagaggt
    12061 tgcgagaaat gacccaaaaa cgcagagaca gaacagagcc aaaatataaa gcgttagaca
    12121 tgactgagca caccttaaag gtggcaatca gaacgataga ccgccacacg cgggaaggat
    12181 acgcgaagga acatcccgac ctgataagcg cattcatgac cacggcggcg gcaaactttg
    12241 ccacgctgac agaacgggag attgccgaag cggaacaggt aacaaccatc aacgttaaaa
    12301 ccggagaggt ggaattatga cggcacagat agccgcttac gggcggctgg tggacgaccc
    12361 gcaggtaaaa cagaccagca agggcacacc gatgacgctg gcgcgtatgg cggtatcttt
    12421 gccatgcagc caggcacagg acggacaggc gacgttatgg ttatcggtca tcgcatttgg
    12481 caaacaggcc ggcttcctgg ctaaacatca aaaaggcgat gttgccagcg tatccggcac
    12541 gatgcaggta agccagtgga ccggacagaa cggagaaacg cggcagggct ggcaggttat
    12601 cgcagacagc gtaatcagtg cccgcgcggc acgtccaggc gggaacagac gcaaaaccac
    12661 aggcacacag ggtaatcagc caccagcggg aggcgatgac ccttacggtg atgatatttc
    12721 gttctgaggg ggtgacgatg gtacatgacc gcatagcgga ggaactggag gcgaaaggct
    12781 tttaccggag ggcggcggcg cgatggggtg aagtcatgca gctggtggag acagacaagg
    12841 aacggcatca catcacgatg cgacggctgg aatgttcaag gaaggcacag agggcaccgg
    12901 agccgccgga taatttcgga gacctgaaaa aggcagtcga tcgcacttat gccgaaatgg
    12961 gtatagatgg tgctggtgat gaaatatggc gcaattacca ggacagctaa tcaaacagcc
    13021 ggagaaatcc ggcttttttt gcaccagttg aaacggtatg gcgcattacc gggttttcgt
    13081 cacggtcagg catagttact atctgaaaca aacagacaca acagaggaaa aaaacaatgc
    13141 cgatgaaatt tgatgagata ttaaaacagc gtgataaata ccatgctgac aacatggaga
    13201 cgatgagcat caatgattac cgcgcattcc tggagacggg cgcactgatt gaaaaggatc
    13261 agcatggttt tgtgagatgt gctctatccg gtgaaatgct ggcggtaaat cctgaacaga
    13321 tagatgcatt gatagaattt ctgaaagaga tcagagactg agccagcaca cagcacacat
    13381 agccggagca atccggcttt tttgcgccca aaaaaagccc gataaggtca gagggttctt
    13441 atcgggcttt tgcatatgag gtttttggat gcactggcgt tcgtgatcgg gataatcatt
    13501 tcataatttg caacataact caatttcatt gcataaaatg caattctgat tataatcagg
    13561 actggataaa catccagtta tgattttttt agtcgaagag gaatttctta ctatggctga
    13621 agagaaaaaa agcggtgttt cggtgtacat aagccccgaa atcgtggagg tgctcaagca
    13681 gcgccacaaa aaaaactatg aagctggcgt tgcggctgga ctggatccgc tgatgacgcc
    13741 ggagccgtcg ataggttcac ttgtacgctc ttatttactt gcggcgcttg ggatgcataa
    13801 aaattatggg ggtgaataat ggcaggcaaa gcaacggcac ttaacactaa ccagcttttc
    13861 atgtacctga atcgcgggga tattgcggat tttaaattca gccctctgtt taccacgctg
    13921 tttttcccga acgtggcgac attcagcacg caaaacatca tgctggatac cctggacatt
    13981 gaagaagtca ccatgtcggc gttttgttcg cctatggtgg gtagccaggt tcagcgcgat
    14041 aaagggtacg aaaccagcac aatcaaacct ggctacatga agccaaagca cgaaatcgat
    14101 ccaacgaaaa ccatcatgcg catggctgga gaagatccgg cacagcttaa cgaccctacc
    14161 tatcgccgta tgcgcctgat tactggcaac atgcgccgcc agataaacgc cattaaggca
    14221 cgcgtggaat ggctggcggt gaatgcggta acgaccggaa aaaacatcat tgagggcgaa
    14281 ggcatagaac gctatgaaat cgactggaaa ataccggaaa actgcatcat agagcaggcc
    14341 aagggtaaaa aatggtccga gcaggataaa gacatgcacg acccaatcta tgacatcgag
    14401 ctttatgctg atcaggctgg ttgccccgca aacgtcatga ttatgggcgc tgaggtatgg
    14461 cgcacattac gcagctttaa aaaattccgt gagctgtacg atctttcccg tggttcagaa
    14521 tccgccgccg aactggcctg taaaaacctg ggcgaagtgg tgagctttaa aggctatctt
    14581 ggtgatctgg cccttatcgt ctattccggc aaatacactg acagcgatgg taccgaaaaa
    14641 tatttccttg agcctgattt gctggtcctg ggtaacacca acaataaagg gctggtggcc
    14701 tatggtgcga taatggatca ggaagcggta agaacgggcg caacacaaaa catgttttac
    14761 ccgaaaaact ggattgagga cggcgatccg gcgattgagt acgtgcagac acacagtgca
    14821 ccgcagccgg taccggcaga tattcgcaaa tttgttaccg tcaaaattgg ttaacggggg
    14881 gattctatgg acactccata cattgagtta tttgcaggca gtcagcaggt atccacgacg
    14941 ttggtacatt ttgccgctga tgctggcgtt attcaggaat ttaccccgct gatgctggca
    15001 gacaatggcg agtttaagcc gtgggatggt caggaatctg gcaaggctgt ttatctgacc
    15061 tcgtaccctg tggacacgtc aaagcagaaa tcagcacagt gttacaagac ggggatattc
    15121 aatattgccg ccgttaactg gcctgagagc gtcgacaccg atgcgaaaaa atgcgccgcc
    15181 tttgcgggtt ccggcgtatc cgttcagccg ctggcccgat aagcaggggg aacgatggca
    15241 acgaatgaaa gcatcatgac gctaccgctg gcgagtaaat ttaaagtcga agcgcgggca
    15301 atggctgaca gaggtttatc aacctatgag gccgtatatc aactaaacaa actggaagag
    15361 caggacaagc cgtgcgctga tgctattatg gcgcttcatg agcataaaga ctatcagccg
    15421 ctgttgcgtg caatggcaaa cgtgccttgt atcgatgttg gtacggctaa aagcatcctt
    15481 agcatgacca tagagcagga acgtccgaag gttgcgccgg aacttaccgc agcctttgaa
    15541 aactttatgg acatgcacag cccgcaagcc gtatcagctg gcatggcata cgatggcaga
    15601 aaccagggcg atgacggcga catcgatcgc atactgaaaa ccatctgaga caaggccgga
    15661 gaaatccggc ttttttacgg gtccttcctg gaattatggc ccgttacggg gcggcgacct
    15721 cgcggatttt cgctatttat gagccttttc agggtggtgg tggtggtttt gttgtttgat
    15781 ctatctttat gaatgaaaag ggaaagatgc aagcaataca ccaacctgaa gcagtaatta
    15841 agttggtgta ttaatgaaat ctcatctgat gaacaaaaaa aacatggcga aaagctgccg
    15901 tgtaagtgcg acagcgttcg acaagtgggg agtaactccc gttgagcgta aaggccgtga
    15961 ggcgttttat gatgttgcca gcgtgataga aaatagggtt aacaatgcaa ttagccagct
    16021 tataaacgac aaaggcgaga ttgacgatga tgaactttta cgagtcagga tcagattact
    16081 gacagcacag gcagaggcgc aggaacttaa aaacgatcgc gatcgcggtg acgtgattga
    16141 tactgagttt tgcctgtacg cgctttcaaa gctggcgagt cagatttcat caatcatgga
    16201 cagcctcccg cttactatgc aaaggagctt cccacagatt acccccgcca tgctggacag
    16261 gcttaaaagg gaagtggtta aagcctgtaa tgccagtgcc agagttgccg acaacctccc
    16321 acagatactg gctgattact tgaaagaaac aaccggaaac gtaccggaaa agttgctaca
    16381 gaagaaaggc gagtaacaga cgcgcaatta ttgaacaaaa atgagaaagg atctgataat
    16441 ggcattttct ggctaaaatg cctcgtgtca gatcctttct ttttttaata ttttcattgt
    16501 taaacagtct gttacagaga agaagatcgg atctgacttt tgccggaaaa ttctcataaa
    16561 cagataaaaa ccgcgaggtc gccgccccgt aacgggccat aattccagga aggacccgac
    16621 gacaccagac tatcagagcg atgggggcac aatgacagaa gccgaactac tgggattaat
    16681 ccgccgcgtc gccggaatca gccagcagca tgacgaacag gccacacagc cggacagcgt
    16741 gaccgctgaa aattatgtgc gtgttgtagc ggaagtgatg cgccgtgatg gtatagaact
    16801 taatggcgtt gatatgcgca acatacggaa ccgcgttctt gaaatgctgg cctacaatcg
    16861 ccgcgttgaa ctgtatcggg agaaagaaaa aataacgtac cactggaaga agccggaacg
    16921 gctgcggcgg taacttgctg atattcccga taacgcaaaa ttgcgctggc tacatcctca
    16981 catctgagga ctgttaatca gaaggccgga atatattcac aaactacgaa attttcgtag
    17041 tttaattaat ggtcagatta atcgctaacc cgctgatatt ttcggaaacc tcaatttgag
    17101 gaagtcggaa gcgtcaccct tcatattctg aatggtcata atgaccacgc agaccatcaa
    17161 ggcaaccaca acgaacatcg ttacggttgg agcttttacc ccactggggg aaaggtatta
    17221 cgataatcat aacacctacc gaacaggtaa aacccaccag cctgattaac agttaaccgg
    17281 aaaaaaaatc cggtagcccc gttatgggga tccccatatc gacattaacg cccctcatga
    17341 attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt ctatttgttc
    17401 cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa tttgctgttt
    17461 ttagcacttt ttgcagtgtg ggttgtttaa ttaaatcaat gggttgcctt gctgtggtga
    17521 taaaaggaca tatttattca tctttcggcg tagaatgctg gcgaccaaaa atcacctcca
    17581 tccgcgcacc gccaagcatg ctctctccgg cgacgatttt accctcatat tgcttggtga
    17641 tttcgcgggc taccgccagc cccacacctt gcccagggcg taaagtatca acccgttgac
    17701 cacggtcgaa aatgacctct cgcttgctta atggaatacc ggggccatca tcctcgacca
    17761 caatatagag atgctcgtcg gtttgccttg cagaaatttc gacaaactcg aggcaatatt
    17821 tacaggcatt atccagtaca ttgcccatca cctcgacaaa atcgttctgc tcaccgacaa
    17881 agctgatctc tggcgaaata tcgagagaga tattgacccc tttgcgttga tacactttgt
    17941 tcagcgccga ggtgagattg tccagcagtg gggcgaccgg atgcagctcg cggctgagca
    18001 atgtcccgcc gtgcatactg gcacgatgca ggtagtagcc aatttgctgt gaaatgcggc
    18061 tgatttgctc cagcattacc ggttcagcat cactgacgct catcttttca ctacgtagag
    18121 aacgcagcgt actttgcagc accgccagtg gcgttttcag actatgggtc aggtcggtga
    18181 gcgttgtacg atatttgtcg taacgttcgc gttcactttt taacaatcgg ttcaggtttc
    18241 gtaccagact ggtcagttct cgcgttgtgg ctggattgag caattcgcgg ttatgttctt
    18301 ccagttcgcg gacttctttt gccagggctt cgatggggcg taaactccac caggcggcga
    18361 cccacagcag cgggatcact aacagcagat tggctgagag cacatagata aaccagctcc
    18421 agaccatata ggaacttttt agctccaccg gaatggtatc caccaccaca atggttaact
    18481 ttggcatccg cgatgttgcc gggtagacgt ttaccgccac cgagtgggtc atctccgcgt
    18541 cgtcatcatc ttcccgcact tcctgcaact gttgctgtat cgaatgatct ccactcagca
    18601 agaggctggt atcgttaaca tccgcttcaa tttcatgaaa accattcgat ttcagccagt
    18661 caggctggat catcttcatc agccagggca cgtcacgttg cgcccataaa agctgcccgt
    18721 tctcatcata aattagcgtc atggtggggc tttgcttgtc gatattttcg ggtaactcga
    18781 catgcaactt attgttttcc cacttcgcaa gggtatagaa cagattgctc tcgccacgta
    18841 acagccgaaa cgtagtttta tcgaaactga cgctataacc gatcagcgcg accattccgt
    18901 aggcaagcga aagcaccagt actaccgctg ccgttgccaa cagaaaacgt acccgcagcg
    18961 agagcgggaa aaaaagacgc agtaattttt tcatcagcgc aattcgaaca aatagccctg
    19021 gccgcgaacg gtagtaatca cttcttgcgg atattgtgcc tgaatttttt tgcgcagacg
    19081 tcccatcagt acatcaatgg tatggctttc ccgcagctcc gcatccggat agagttgtag
    19141 cattaacgaa tctttgctga ccactttgcc attattgcgt atcaacgttt ccataatggt
    19201 gtattcgaac gcggtcagtt tgatcacttc gtcattaata gataattcac ggcgagagag
    19261 atcaacctga aacgggggga gcgaaatgac ctgtgaagcc aggccgctat tacgccgcat
    19321 taatgcctgc attcgcgcca tcacctcttc aatatgaaac ggtttagtca cgtaatcatc
    19381 agcaccggca cttaatactt cgactttgtc ctgccagctt tcacgggcgg ttaataccag
    19441 aatcggcagt gaaacatcat tgctacgcca gcggcaaatc agtgacagac cgtcctcgtc
    19501 tggcaatccg agatcgacaa tcgcaatatc cggtaaatgt tcattgagat aataatcggc
    19561 ttctttggca tcttctgcat catcgacctg atgaccagca tcctgaatct gaactttaag
    19621 gtggtgacgt aacaacgcat tgtcttcaac aaccagtacg cgcattttta tttctccctg
    19681 tcttaattat taaaatagtg taacgcgatt atgtggttat gggggtaaac attaaataaa
    19741 ccagcggggg aggggaggta aagtgaaaaa ataaaaagcg gataatctta ataagcaggc
    19801 cggacagcat cgccatccgg cactggtacg agtttatttc agctcatcaa ccatggtgat
    19861 ggcgcgacca atgtagttag ccggcgtcat cgctttcagg cgggctttct cttcttctgg
    19921 caacgccaga ccgtcgataa actgcttcat gccttcggcg tcaacgcgct taccgcgagt
    19981 cagctctttc agcttctcgt acggtttttc gatgccatag cgacgcataa ctgtctggat
    20041 tggttctgcc agcacttccc agttgtgatc cagctcatcc agcagatggt cacggttcac
    20101 ttccagtttg ctcacgcctt tcagggtgga ttgatacgca atcagcgcat aaccgatacc
    20161 cacgccgagg ttacgcagca cggtggagtc ggtcaggtca cgctgccagc gggaaaccgg
    20221 cagtttgctt gccagatgct gcaataccgc gttggaaagg cccagattcc cttcggagtt
    20281 ttcgaagtcg atcgggttaa ctttatgcgg catggtggaa gaaccaatct caccagcaat
    20341 ggttttctgt ttgaagtggt taagggcgat ataaccccag acgtcacggt caaagtcgat
    20401 cagaatggtg ttgaagcgcg caacgcaatc aaacagttcg gcaatgtagt cgtgcggttc
    20461 aatctgggta gtgtacgggt tccattgaat acccagcgag gtgacgaact cttcgctgaa
    20521 ctgatgccag tcaacttccg ggtaagcggc gatgtgggcg ttatagttac cgaccgcgcc
    20581 gttgattttg ccgaggatct ccacctggtt aagctggcgg tactggcgct ccatacggta
    20641 ggcgacgttt gccatctctt taccgatggt tgacggcgtg gctggctgac cgtgggtacg
    20701 ggacagcagc gggatatcgc gatactgaac ggcgagatct ttaatgccat caattagttg
    20761 acgccagtac ggcaggatca cttcatcacg cgcggttttc agcattaatg cgtgggagag
    20821 gttattgata tcttccgaag tacaggcaaa gtggatgaat tcagaaaccg cgtgcagttc
    20881 cgggatctcc gccacttttt ctttcaggaa atactcaacc gctttaacgt cgtggttagt
    20941 agtacgctcg atggttttga tgcgtgcggc atcttcttcg ctgaaattgg cgacaattgc
    21001 atcaaggtaa ccgattgcgt cggcagcaaa agcaggaact tccttgatcg ctgcgtgcgc
    21061 ggccagtttt tgcagccaac gtacttcaac ttgtacacgg aatttcagca aaccatattc
    21121 gctgaaaatc ccgcgcagcg cgctgacttt atcgccgtag cgtccatcga caggggaaac
    21181 ggcggtcagt gaggataatt ccatagatca caactccggg gttaaatgag caagaatttg
    21241 ttttgcctga gtggtcaggc gattacgaga aaacatcagt tgcagacgtc cgccgccgac
    21301 ctggtgccag agcacggcgg cgcgaatgcc tgccagcagg gttgcgcgaa ctttcgcctg
    21361 cacttgtggg ctttgcagta cagcagggga accggtgacc tgaatgcgcg ggccaagcgg
    21421 gctgatcaca tcaacataga tagcagccat cgcgctcatc agcgtttcgg actgtaaatc
    21481 gaagtgttcg agctggcgtt gcaggccgtt gatccggttg cccagagtgt cgagcgcacc
    21541 tttcgctgag gagagtttgc gcttaagcac catcaggctg agtgtgtagc gggttaattc
    21601 ggcgtttaag ccctggcgac tgctggcatt gagcacgccg agcagggttt ccagcccgac
    21661 gcgcaggttg gcttcgctac cgccaaaaac cgccagcgtc gagctggggt tcatatcaat
    21721 aatactgttg agtgagacgt gtagcgcatc ggcatcacaa tgcccctggt gagcgagttg
    21781 ttgcaccagg cgtgccgact gacaaatacc ggccagggcg agggtgatgt catagtaatt
    21841 ctttgccacg ttcactgctt ccttgtttta agtaaagata ataatcagat cggcagcggc
    21901 agacgctgct caataatacc gccaccgagg cacacttcac cgttatagaa gacggcagac
    21961 tggcccggcg tcacggcggc aacgggctca tcgaaaatca cttcaatgcg atcatcgtcc
    22021 agcgccttga cggtgcaggg gatatcggtc tggcgatagc gggtttttac cgtgcaacgc
    22081 atagtaccgg tgaatggttc acgatcgacc cagtgcagct gctgggcaat cagaccgaca
    22141 gacatcagac gcgggtgttc atggccctgc gcaacaatca gaatgttgtt ttcgacgtct
    22201 ttatcgacca cataccacgg ttcttcggtg ccttctttgg tgccaccgat acccagacct
    22261 ttgcgctgac cgagggtgtg atacatcagc ccctgatgct caccaatttc atcgccatcg
    22321 actgtaatga ttttgcccgg ttgcgccggg agataacggc caaggaactc gcggaattta
    22381 cgttcgccaa tgaagcaaat gccggtagag tcttttttct tcgcggtgac cagaccaaga
    22441 tcttcagcaa tcttacgcac ctgcggtttt tccagttcgc cgaccgggaa caggctttgc
    22501 gcaatctgct catggctgag cgtataaagg aagtagctct ggtctttatt gctgtccaga
    22561 ccacgcagca ggcggctctt gccatcgaca tctgcccgac gaacgtaatg accggtagcg
    22621 atataatcgg cacctaaatc ttcggcggca aattcgagga aggctttaaa tttgatctct
    22681 ttgttgcaca gaatatccgg attcggcgtg cgaccggctt tatattcggc aaggaacagt
    22741 tcgaagacgt tgtcccagta ctcggcagca aagttaacgg tgtgcagttc aatgccgagc
    22801 ttgtcgcaga cagcctgggc atcagccaga tccgctgccg ctgtgcaata ttcctcaccg
    22861 tcgtcttctt cccagttctt cataaacagg ccttcgacct gatatccctg ttgttgcaac
    22921 agccaggcag aaacggagga atcgacaccg ccggacatgc cgacgattac tttttttgcg
    22981 gtttcagaca ttggatcact cacgacattg aacttcaagg cggcgtattc tatcacgcag
    23041 ccttttagat gacacccttt gtaaaaggcc agttaaaatc gccaatcatc tccagcggat
    23101 agcgttgccc gctttgataa caacggatac tttccgccac cagcggcgaa cgaagatttg
    23161 acgcctgtaa aatttcttcg gcgctgaccc aacgacagca gtcgatatcg ctgtcatgag
    23221 gctgggtcgg gcatatttgc tcaagctcaa tggcaaagag gaaacgcaaa aacggcgttt
    23281 tatctggcgc aatccactga tgcatacgaa taaagtgttg cggctgcgcg ctgatgccgg
    23341 tttcttccca cagctcacgg gcggctgctt ccactaaggt ttcatcggct tccagatgcc
    23401 cggcaggttg gttccataag gctttaccat taatcgtctc ttcaacgact aaaaatttcc
    23461 cctctgcgtg cactacgcaa gcaacggtaa cgtgcggttt aaacattgtg catccttatt
    23521 ctgttgcttc tcgccattcg ccattggcaa ggttatccaa agagtaatca cccatcgcat
    23581 agcgaatcag tcgcagcgtg gggaaaccaa catgggcggt catgcggcgc acctggcgat
    23641 tacgtccttc atataaggtg atcttcagcc agctggtggg aatacttttg cgttcacgaa
    23701 ttggtggatt ccgcggccat aaccacgcgg gttcgtcaac cagctccgcg cctgcgggca
    23761 gggtagggcc atcatttaag gttacgccat tgcgcaaggc ttcaagtgcg tcttgtgtgg
    23821 gaataccttc cacctgcaca taatagattt ttccggtgcg tttgcccggc tgggttaaac
    23881 gcgcctgcaa cgcgccgtta ttggtcagca ccagtaaccc ttcgctatcg cggtcaagac
    23941 gacctgctgc ataaacgccc tgaaccggga tgaattcttt taatgttttg cgtccggctt
    24001 catcggtgaa ctgcggaaga acatcgtagg gtttattgaa caggatcaca cgcgtgggct
    24061 ggttttcagg tttacgcctg gtagaacgtt gtgagctgaa tcgcttaacc tggtgatttc
    24121 taaaagaagt tttttgcatg gtattttcag agattatgaa ttgccgcatt atagcctaat
    24181 aacgcgcatc tttcatgacg gcaaacaata gggtagtatt gacaagccaa ttacaaatca
    24241 ttaacaaaaa attgctctaa agcatccgta tcgcaggacg caaacgcata tgcaacgtgg
    24301 tggcagacga gcaaaccagt agcgctcgaa ggagaggtga atggaaagta aagtagttgt
    24361 tccggcacaa ggcaagaaga tcaccctgca aaacggcaaa ctcaacgttc ctgaaaatcc
    24421 gattatccct tacattgaag gtgatggaat cggtgtagat gtaaccccag ccatgctgaa
    24481 agtggtcgac gctgcagtcg agaaagccta taaaggcgag cgtaaaatct cctggatgga
    24541 aatttacacc ggtgaaaaat ccacacaggt ttatggtcag gacgtctggc tgcctgctga
    24601 aacccttgat ctgattcgtg aatatcgcgt tgccattaaa ggcccgctga ccactccggt
    24661 tggtggcggt attcgctctc tgaacgttgc cctgcgccag gaactggatc tctacatctg
    24721 cctgcgtccg gtacgttact atcagggcac tccaagcccg gttaaacacc ctgaactgac
    24781 cgatatggtt atcttccgtg aaaactcgga agacatttat gcgggtatcg aatggaaagc
    24841 tgactctgcc gacgccgaga aagtgattaa attcctgcgt gaagagatgg gcgtgaagaa
    24901 aattcgcttc ccggaacatt gcggtatcgg tattaagccg tgttctgaag aaggcaccaa
    24961 acgtctggtt cgtgcagcga tcgaatacgc aattgctaac gatcgtgact ctgtgactct
    25021 ggtgcacaaa ggcaacatca tgaagttcac cgaaggcgcg tttaaagact ggggctacga
    25081 gctggcgcgt gaagagtttg gcggtgaact gatcgacggc ggcccgtggc tgaaagttaa
    25141 aaacccgaac accggcaaag agatcgtcat taaagacgtg attgctgatg cattcctgca
    25201 acagatcctg ctgcgtccgg ctgaatatga tgttatcgcc tgtatgaacc tgaacggtga
    25261 ctacatttct gacgccctgg cagcgcaggt tggcggtatc ggtatcgccc ctggcgcaaa
    25321 catcggtgac gaatgcgccc tgtttgaagc cacccacggt actgcgccga aatatgccgg
    25381 tcaggacaaa gttaacccag gttctattat tctctctgct gagatgatgt tacgccatat
    25441 gggttggact gaagcggctg acctgattgt taaaggtatg gaaggcgcaa tcaatgccaa
    25501 gaccgtaacc tatgacttcg aacgtctgat ggaaggcgct aaactgctga aatgttcaga
    25561 gtttggtgac gcgatcatca agaatatgta atcactacat gtgttaaata ttgaaacggg
    25621 cgtataacac gcccgttgtt ttatgatgat ttaaaatctt ccccaaaact ttccccaaaa
    25681 cccttcccca aaactggcta ttttctatgc tgttttgata tctacgataa tccagtcttt
    25741 accacgatca tcattgtatc ggtcggtcat ttttctggat ttatgaccta acaacttttg
    25801 cgtatccaga ccctgttccc gatataaccg ttctgacaga gatcgctgct catgaaatgt
    25861 aggcgcagtt ccttgctccc attttatgcc acatttttcc ctggcctttt taaaagccgt
    25921 tgtaagagta tttgcagaca cctggtctcc tctgtttgct tgagaggtag tgtgacggta
    25981 atggaccaga tatttactaa caacagcatc cctgcactga gatataactt cacgaagggt
    26041 aatattcaga gcatcgcatt tcaggttaag cggaatagca agttttgaac cggttttttc
    26101 ctgagtaatg tgcaacatgt cgtcccagat atcagagaat ttcaaattgc agatatcgct
    26161 taaacgttgt ccagtgacaa gagcaagtag catcccgcat tttaaatagg gctgccgtct
    26221 gcttacgctg tcaaatattg cctgccattc gggcagtgat aatctttggc ggtttactcg
    26281 atttcgcggt tgttttgttg cctgcgctgg gttaaatcct ggcggaacat gtcctgcgtg
    26341 ttgtgcttct ttgaagacgt cgatcaacac cattctcacg acttgcgcca tcctgttatg
    26401 accttcagcc tttacagcat caattatttc ggcaatatca agtgcggtaa tatccttgag
    26461 gtgttgcatt ccacaatgct cacggaaaag acggatgggt ttgccttttt gccgatagga
    26521 gttgggtctt agttcattat gttgcagcct gtcctcctgg atagaattat atttatcaag
    26581 ccattctgtc accgtaatgt ctgagcgcct gcctttcatt ctttccagac gctcattgac
    26641 gcttaatatt tgtcgggtac gttgttcagc aataatggta tttgcttcag tagcaacttg
    26701 ttttgcttca ttctcatcag ttcctaagct atgaaaacga ccggatagtg gatgtttgta
    26761 ttgccaatat acctttccgg ttcgcttatc taatttgcaa tataaattgg gtatagagat
    26821 tttgtgagat cgggggctag cagccatcag cgattatccg ttggagtttt gggtttgcgt
    26881 ttattgggag ttgcggttct gcaagcgttc ctacaaaacg ggaatttcgg tcaatcatcc
    26941 agtagcgacc aacttttata gcgggtgggg ccatcatttt cccttgcgcg tattttttca
    27001 gaactcgctc acttggtgct aagtccccaa attcttcttt agcccagtcc cgtaaagtga
    27061 ttagtcgaga catttgtcct cctcttagct gctgagggag tttgtgaccg atatatctga
    27121 catgatatta agctcatggc aggtacatct cttgactggt catagagata aatttaatgc
    27181 tgagaaatgc agtattgaat ttatcaattt ttcaccagct tctggtcttt ctggctgtca
    27241 tgcgcaaaac atatggcttt aataaaaaac tggattgggt tagcaacgag caactttccg
    27301 aattaaccgg gatattgccg cacaagtgtt ctgctgcaaa aagtgttctg gtaaagcgtg
    27361 ggatttttat tcagagcggg cggaataccg gcattaataa tgtggtcagt gaatggtcaa
    27421 cattacccga atcaggtaag aaaaataaag tttacctgaa agaggtaaat ttacctgaat
    27481 caggtaaaaa aagtttaccc aaatcaggta aaggcgttta cccgaatcag gtaaacacaa
    27541 aagacaaact aacaaaagac aatataaaac ctttttcgtc cgagaattct ggcgaatcct
    27601 ctgaccaacc agaaaacgat cttcctgtgg tgaaaccgga tgctgc
//
LOCUS       DAZFBR010000065        27490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFBR010000065 DAZFBR010000000
VERSION     DAZFBR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.065"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..2885)
                     /locus_tag="ACHJZ2_002915"
     CDS             complement(<1..2885)
                     /locus_tag="ACHJZ2_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4568989.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSTLWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAVNLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYIYRYDEYGRLAEKTDR"
     gene            complement(2961..5102)
                     /locus_tag="ACHJZ2_002916"
     CDS             complement(2961..5102)
                     /locus_tag="ACHJZ2_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO4568990.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIASILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVLYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQILNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            complement(5312..5830)
                     /locus_tag="ACHJZ2_002917"
     CDS             complement(5312..5830)
                     /locus_tag="ACHJZ2_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO4568991.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQNPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            6527..7027
                     /gene="tssB"
                     /locus_tag="ACHJZ2_002918"
     CDS             6527..7027
                     /gene="tssB"
                     /locus_tag="ACHJZ2_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO4568992.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            7062..7286
                     /locus_tag="ACHJZ2_002919"
     CDS             7062..7286
                     /locus_tag="ACHJZ2_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4568993.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            7337..8728
                     /gene="tssC"
                     /locus_tag="ACHJZ2_002920"
     CDS             7337..8728
                     /gene="tssC"
                     /locus_tag="ACHJZ2_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO4568994.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            8819..9232
                     /gene="tssE"
                     /locus_tag="ACHJZ2_002921"
     CDS             8819..9232
                     /gene="tssE"
                     /locus_tag="ACHJZ2_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO4568995.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            9236..11086
                     /gene="tssF"
                     /locus_tag="ACHJZ2_002922"
     CDS             9236..11086
                     /gene="tssF"
                     /locus_tag="ACHJZ2_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO4568996.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            11050..12132
                     /gene="tssG"
                     /locus_tag="ACHJZ2_002923"
     CDS             11050..12132
                     /gene="tssG"
                     /locus_tag="ACHJZ2_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO4568997.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            12157..13437
                     /gene="tagH"
                     /locus_tag="ACHJZ2_002924"
     CDS             12157..13437
                     /gene="tagH"
                     /locus_tag="ACHJZ2_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO4568998.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            13434..13958
                     /gene="tssJ"
                     /locus_tag="ACHJZ2_002925"
     CDS             13434..13958
                     /gene="tssJ"
                     /locus_tag="ACHJZ2_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO4568999.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            13961..15292
                     /gene="tssK"
                     /locus_tag="ACHJZ2_002926"
     CDS             13961..15292
                     /gene="tssK"
                     /locus_tag="ACHJZ2_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO4569000.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTIFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            15297..16058
                     /gene="icmH"
                     /locus_tag="ACHJZ2_002927"
     CDS             15297..16058
                     /gene="icmH"
                     /locus_tag="ACHJZ2_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO4569001.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            16067..18850
                     /gene="tssH"
                     /locus_tag="ACHJZ2_002928"
                     /gene_synonym="clpV"
     CDS             16067..18850
                     /gene="tssH"
                     /locus_tag="ACHJZ2_002928"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO4569002.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDA
                     QNDESVTKDETVL"
     gene            18847..19590
                     /gene="vasI"
                     /locus_tag="ACHJZ2_002929"
     CDS             18847..19590
                     /gene="vasI"
                     /locus_tag="ACHJZ2_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO4569003.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            19595..21007
                     /gene="tssA"
                     /locus_tag="ACHJZ2_002930"
     CDS             19595..21007
                     /gene="tssA"
                     /locus_tag="ACHJZ2_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO4569004.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHAQLDIPELQRRGLQLLASESKDFRLLAHLLRTLQHAGDPLLALHLLTLYVEH
                     YWTVAAPQNMAHKKRFASQIIKRFETGIEGFSQNAATTQRDTLLGELAKLAQCWQSHN
                     VPELAQATDDLFALYQRTFNRAAPAPVPTPAASGSSPQTTVTSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            21144..24578
                     /gene="tssM"
                     /locus_tag="ACHJZ2_002931"
     CDS             21144..24578
                     /gene="tssM"
                     /locus_tag="ACHJZ2_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO4569005.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            24589..25998
                     /locus_tag="ACHJZ2_002932"
     CDS             24589..25998
                     /locus_tag="ACHJZ2_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO4569006.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNTIPTIKKALNFNN
                     IHEYKGVLNGEFNLFNTKW"
     gene            25964..26443
                     /locus_tag="ACHJZ2_002933"
     CDS             25964..26443
                     /locus_tag="ACHJZ2_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO4569007.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELFYK
                     IKLTGAKISDIHVSISHIVVDNSVQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
     gene            26464..26685
                     /locus_tag="ACHJZ2_002934"
     CDS             26464..26685
                     /locus_tag="ACHJZ2_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569008.1"
                     /translation="MANNILHSSTSDSESKNTSTDSTLFNEKFPFQLRQDFASRITED
                     ESDFFKWKKIRELAFVSNHKEWNKYDLLI"
     gene            26764..27360
                     /locus_tag="ACHJZ2_002935"
     CDS             26764..27360
                     /locus_tag="ACHJZ2_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO4569009.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSAIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASMKVIITG
                     WKPITIKNVAMNYNGGGDPKYAIKLQFVYTLLTKNRVL"
     gene            27363..>27490
                     /locus_tag="ACHJZ2_002936"
     CDS             27363..>27490
                     /locus_tag="ACHJZ2_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569010.1"
                     /translation="MRKITIILLLTFSCTKGLAFDISMSKECSGVDELEFIDCVKNS"
BASE COUNT         6392 a         7672 c         7344 g         6082 t
ORIGIN      
        1 cggtccgtct tctccgccag cctgccgtat tcatcgtagc gatagatata gtgcgcatct
       61 tccgcgatgc ggttatccgg ccacgccgtg agcgtgctgt ccggatgcag ttccgggtcc
      121 ggcagccggt ttcctgccgg gtccgttgca tacgggatat cgatatccag gttcactgcc
      181 gtggtgtgga cgcccgtcag ccttcccgtg tcactgtaac ggtactcccg gctctcctgc
      241 gggccgctga tgcggattag ctgtccgttg tcgttccagt cgtagtcacg gtcaagctgc
      301 ggaaggttca ggtgcctgct ccggagctgg ccgctggtat tccaggcggt ggcaagttca
      361 tatgcctccc cgccgaagct gcgggccgtc tcacggtgca gccggtcccg catgtactcg
      421 accagtggcg ttccgcccag cttcatcccc gcaagataac cgctgccata cgtcagccac
      481 tctaccggcg gcagaccgtc cggctcctga cgggtcgcca gcccctgttc gctgtacgcg
      541 tgccccgtct catgctccca cagcatctcc cccgtctccg ggttctccac cgtctgccgt
      601 tcacccgtca ggcggccttt atcgtcatag ccgtagtgga ccgacacccg gtggccttca
      661 ctggtatggc tgagggtggt tagccacccg tgctcatcat actgccactg ctctgccggg
      721 tcgccgttca ccgtccggtg cgtgatgcgg tccgacgcat cgtagtgcca gagggtgata
      781 agcccctcgt cttcactctg cgtgagtttt ccggtcaggt catagtggta acgttgcgtc
      841 cggccgtcaa aaccgcgctg ttcagtcagc ctgtccaccg ggtcataccg gaacgtggac
      901 tggctgccgt tctcgttggt cagcacggtg atgcgcccgg cagcgtcata ccccatgctg
      961 cgcgtcagac cgccctgcgt ggtgctgacg gcctttcccc acgcatcata ctgtatctca
     1021 ctgcggctgc cgtccggggc aacgatagcg gtgaggtcgc ctgcggcgct gtactcataa
     1081 cgggtttcac gcccctgcgc atccttctga ctgaccagtt ggccacgcgg gttataactg
     1141 ctgtaagtgc tgatgccttc ttcccggtga acggcgattt gttgaccgta ccggtcatac
     1201 tcataccgcg tcgtgtaccc cgagcagtcc gtaaaggtca gcagctgacc gtaacggctc
     1261 catgccatct gttttgtact gcccgtcgcg tcctgtatcc ctgtcggcag ctcactcgcc
     1321 gggtcatcat agctgtaccg cgtcgtctct ccgctgcgcg aggtctccgc cgccagcctt
     1381 cccttctcat catactcccg gctgctgcgc agcccgtccg ggtacgtcac tgacgtcacc
     1441 tgccgctggc tgttatagcc ataccgcacc gtcctgccgt ccggccccgt caccgctgtc
     1501 accgcacccg acgccatatg caggctgtac tccgtccgcc gtcccgccgc atccgtctgt
     1561 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc cgcatgttcc
     1621 ttcttcacaa cacgtttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
     1681 aggctgtccg tgatggtcac acggtcctgc ccgtactcaa agcggtagtc cagcccctcc
     1741 gggttgacca gctccgtcac ccggccggta tcatcatacc ggtagcggct ctccggccta
     1801 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagc aaacccgcgc
     1861 acctgcgtcc cgctgcggtc atacaccgcc cgcagttctc cgctggccgt gtacgtgtag
     1921 cgcgccagcg gcgcggcggg cagttcatcc gggtatgccg ggtcgtgtgt cagccatacc
     1981 gcctccagcc ggataccgtt atcggcaccg tattctgtac cggcgggcag cgtgtcgggg
     2041 aaaaccagag aagaggaagc agaacgggga ccggcagggg aagataaaga cgtggcgcgc
     2101 tgtttacgga atacttccgc ccgctgtgcc tgtgtggtca gcaccaggtg aaaacggcgg
     2161 cccgcgccgt ccgtcacccc cgtcaccgcg cccgccacat caccctccgc cgcccggtga
     2221 aaggccagcg tccgcccgaa gccatccacc acgcccgtca gcacccggta tgcgggcggc
     2281 tccggcggca gcacctcgtc cgcccccggc acccgctccg gccagttgag tatccaccac
     2341 ggcccctgaa ggctgtttgt cgccaggtac atatgcggac tcaggcgaac atcttccggc
     2401 agcacctgcc agagcgtgct tagcggctgc gaactgtgct gctccgccac cccgccccgc
     2461 gccagccaga acgactcgct gcggctgtag cttatctcgc cgggaaacag cggctcaaag
     2521 tgaatgctcc tgccgccgtt gtcgttgagt atcaggcctt catcgcgtat ctgtaagcgg
     2581 atatcgaagg gcgctttcca gccgggacca aacaccccca ccggcgcggg tgttctggtc
     2641 cggtagctgc tgtaggcgcg ggaaagaata aacggcagcg ggccgggcag cgcaaggtcc
     2701 gtctcgcccg gcagcacctt cgcacccagc accgggttca ccgggttagc ataggtaatc
     2761 cctcccggac acaccgagca ggccacgccc gtcggcgcac ctatcagcac ccctgctgaa
     2821 ccctgcacaa tatccagtcc cttgcgggtc atgtcaccct gccgcgccgc cggttttcct
     2881 cccatgaatc aattccttct gcttttaata tggtttatct ggtcactgca ccagttccgt
     2941 tttgattttt tgcacctgac tcaaaattgc cgcatatccg gcattccggg gggtaaatct
     3001 ttcggcacag gcgcatccgg tacaggttgt gtggcaccac agttccagtt aatcaccggc
     3061 gcatcaccgt tcacatccga ctccccttcc agatgaatgt gcgtaccatt aagaaatatg
     3121 cttccgtcct tcgtcagcac cagccttgcc ttaccacagc agagttcaag atgctcaccg
     3181 gcagaataaa ctgctgtttg tccggtgttt tccttcatcg tcttgcccac cgagaaggtg
     3241 acgttattcc ctacattgac gctgtagccc attcccacca tcagtgattt atgcagccct
     3301 acctgtgagg actgcaacaa cgccaccgag gtattcataa tgccgcctac cgtcgtctga
     3361 tacgccacac ccaccgtcag cgcacgaatg atccccacct tttcaacctg gtttgatccc
     3421 actttgataa tctggttact gcctaccgtt tccacctggt tgacaccaac cgtctgtatc
     3481 tgattaacgc caatgttcag gatctggtta ttggtaacgg tgatcgcctg gttgttacct
     3541 attttttcgg catggttgtt tatcacatcc gtggtgcggt tattcagcac ctcggtgttc
     3601 atgttcttct gcgcgtggat gtagacctgt tctttccctg tcgcatcgtc aaacttcagt
     3661 tcattaaacc cgctgccctt ataggttttc gaacgaatgg tcatctgcgt cttcgtcccc
     3721 ggcaggctac cgggtgtgcg gttttcctgg tggtaggtac gccccataat gatcggctgg
     3781 tccggatcgc cgttgaggaa gtccacaatg acctcctgac ccacacgcgg tatcgccagg
     3841 ttaccaaacc cggtgcctgc ccacgcctgt gccacacgga tccagcatga actgtcctgg
     3901 tttgacgggt tatagcggtc ccagttaaat ttcacccgca cgcggccatg ttcatcacag
     3961 aagatttcct cgcctgccgg tcccgtcacg acggcgctct gcgggccgtc caccagcggt
     4021 ttcagcaacg gctgtggtcg ccatgttctg tctgccggta ttaccgcaaa atggttatcc
     4081 agcgtggtgc ctgaacccct gcgtcccggc accgcctgcg gctgctcacc gtgcagttca
     4141 cttgccacca cctgccattc ccggttcagg ttcgcctgcg gatgccccgt cagcacaatt
     4201 cgccgtcccg gccatatctc cggcgaacgg cttgttccgc gcgccacttc tgcgttgttt
     4261 cgccagccgt ccatctgcca gcgggcaaag ttctgcccgt gggcgccctt gaaacgtccg
     4321 ggatagtcat acacttcata ctgtgtgcgc tggtaatcct ggtgctggcc ttcctggtca
     4381 aaacgtccgg cccagccggg gcgtttaaag gtgtagtctt tggtcaccac ggaagaaggg
     4441 cggatttgtg cgctgtagcg aaactggctg atgcagaggg tgcttacctc ggtacgggtg
     4501 ttcggattcc aggggatctc aaaggactcc ggcagataaa gcacggtatc gcacaggacc
     4561 aggctctggt cggtactttt ttgcgcgtgc tcctcataaa agaagatgcc ttcctccgcc
     4621 gccatccggc acaggaaatc gtaatcagtc tcaccgtact ggacacaaaa ctcacgggaa
     4681 ggatgtggct cgctgaacag cgggctccac tcggtcaccc cgttttcctg caatatcgtg
     4741 ccgaggatgc ttgcgatgtc ctcattctgg aagatacgga agttctggcg cagccctgtg
     4801 cgccacagcg gcgggcatac cttcatgctg tacagcttct ggtttttgtc gttctccccc
     4861 agttcaaacc aggtcaccac acctttcacc cggcgctgta cgtcatcgcc ctgccatatc
     4921 gtcaggtagg ccattttgtc gaggatctgc tggaattcaa gggagagaaa ctgctggctg
     4981 accagagaga gatcgaggga aaaaagcgaa gagagtgact ggttcagatg aaaggagacc
     5041 accgcaaaag catccggtgg caggccgtcc acttccagcg tgaaacgtaa tccggttgac
     5101 atagcaacct ccggggttaa ggaaaaacag acagttcgct gttaaagcgg ctgtgacagt
     5161 acagtgctac gcttgcgttc aggcacgcta ccgccgaagt ttccggcagc cgccatctcc
     5221 cgtgaacgta accccttact gacaaccttt ctttctacgg cgtaatacaa aaacagcatc
     5281 cggcctgata gccggatgct gttggcttaa cttatgcttc cagcggtgcg cgccagtcgt
     5341 cggcaccgga ggtgccagat ttgacgtgtt cccattcaat tttgcggtag gccagtgaaa
     5401 cttcgagcaa ctgggtgaac tcgcgtttcg ccgggttctg gcaatgcggc atatgcagtt
     5461 tcatatcgac aatcgtcgaa tcagtcaggc gggtagtgaa ataatgctcc tgtttgcctt
     5521 ccacagaggt acgccaccag tgaagttcag tggttggcag catttcgccg gaggccagtg
     5581 cgttgtacat cagcggaacc gctttgttca gcgccacggt aaaaatgaaa ggcttgtgag
     5641 cacgctgacc cgcaggctga ccggactgag gatcggtcgg gacagtaacg ttatgcagga
     5701 attcctgcac cagcatctcg tcttcgtgcc cctgcacata gatgttgccg acagaatcag
     5761 cggtaaatgc acctgcagta atatttccct gggtctgacc agtgatggag atataacacg
     5821 gtgttggcat ttgtaaactc cttgttaacg tggttaatta ctacttacaa tgacctgccg
     5881 ttaatcccag acaggcagat gcagctcact tcacggaacc gcatcatttt tcagtatttt
     5941 ttacatacaa accgagctag aatttacggt tgtcgtaaaa actattttta aaatcattaa
     6001 atctcttatt cagcaataac ttaataatat atatctggat agccataccc aactgttcaa
     6061 agtccgtatt gcctccataa aatggccaaa gctgaatcat gaataaatat tgtatttatc
     6121 ctttctgaat tatcattttg ataatttttg agatattcct taaaaatacc cacttaatca
     6181 atcacaaaac aaatatataa acaagcaatc attagattag cttatgttta ataaaattat
     6241 tatataattc aataagatac acaaaaaact ctatatttaa attattgcca caatacacct
     6301 gacgcgaatt gcacaggttc ataaaatcta tttaacgctt gcagaatacc cctctttacg
     6361 atcgataaaa agtccttttc ttatttattt tgaagcgtaa aatgcggggt gatgaaggag
     6421 tggtgatttt attttcaccg ccatcaagac ggcatttccg atattttttg cggtacgaat
     6481 atgccgtgtt catggcctgt acgagataac atgacggatc acttttatga gcaaaaaatt
     6541 tgaaggcagc gtcgcgcctc gcgaacgtat taatatatcg tatgtcccaa aaacagatgg
     6601 ccagacggct gaagtggaac ttccgcttaa catgctcgtt gtcggcgata ccggaaatac
     6661 gcaggaaaca tcctcactgg atgaacgtca ggcagtttcc gttaacaagc acaatttcgg
     6721 tgcagtgatg gctgaagcag ccattggcct caattttacc gtccccgcca cgctgaaggg
     6781 aagcacaacg gacgatgaaa tgaatgtggc gctgaatatc aagtcactgg atgatttttc
     6841 accagatagc gtggcccggc aggttccgga agtgaataaa ttgctggaac tgcgtgaagc
     6901 actgactgca ctgaaggggc cgatgggtaa ccttccggca ttccgtaccc agctccaggc
     6961 attgctggaa aatgaagagt cccgcgagca gttgctcaaa gaaatcggcc aggtgagcaa
     7021 taaataacca ccactatttc attaaacagg gttatccagg catgacggca cacaaaaata
     7081 tctccgggac ataccacctc tctgttgcag acattctgca ggtggtttac caggtatgtt
     7141 tcagcccgtc cgttgaaatc aaccaggatg gtgtggctgc gctgattaca acactggatc
     7201 gccgtatcag tgatctattg gatgagatta tccatttttg tgagtttcag cagagcgcgt
     7261 cgcactggca gcgcgtactt cactgacggc gaaaagccgg tttcagaaac acagctaaaa
     7321 cagaaggatt ttcagtatgt ctttacagga agaggaactc gtttccagtc atgccgggca
     7381 gcctgagcag gaatcgtcgc tgcttgatca aatcatggca cagacccgta ttcagcccgg
     7441 ttcggaaggt tatgacgttg cccgccaggg tgtgacagcc tttattgcca gcatcctgca
     7501 atccactgca tctgccgaac cggtcaataa actggccgtg gacagcatga ttgcagatat
     7561 tgatgaacgc atcagccgcc agatggatgt catcatccat gcgcctgcct ttcagcaggt
     7621 cgagtcgttc tggcgctcac tgaaaaccat ggtggatcgc gttgatttcc gtgaaaacat
     7681 caaggtcaac gtgcttcatg tcaccaagca ggaacttctg gaagactttg agtttgcgcc
     7741 agaaattatt cagtccggat tttataagca cgtttattcg tccggtttcg gacagtttgg
     7801 cggtgaaccc atcgctgccg tgctgggcgc ttatgagttc aaaaataccg cgcctgacat
     7861 gaaactgctg caatacgtca gtgccgtggg cgcaatggca cacgcaccgt tcctgtcctc
     7921 cgtttccccg gagtttatgg ggctgaactc gtggaccgaa ctgcctaata tcaaagatct
     7981 ttatgccatc tttgaaggtc cggcctacac caaatggcgc gccctgcgtg actcggaaga
     8041 ttcgcgctat ttagggctta cggcaccccg cttcctgctt cgccagccct attcaccaac
     8101 agataaccct gttaagaact ttaactatta cgaagatgtc agccagaacc acgaagatta
     8161 tctgtggggt aatacggcct ggatgctggc atgcaatatc gcagacagtt ttgccaaata
     8221 ccgctggtgt ccaaacatta tcggtccgca aagcggcggc gcagtgaaag atctgccggt
     8281 gcatctgttc gaaacgatgg ggcagattca ggccaaaatc ccaaccgagg tgctggtcac
     8341 cgatcgccgc gaatttgaac tggctgaaga gggttttatc acccttacca tgcgtaaaga
     8401 ctctgataat gcagcctttt tctctgcaaa ctcggtacaa aaaccgaagc actttccggg
     8461 aaaagatgca gaaaccaact ataagctggg tacgcagctt ccttatctct tcatcatcaa
     8521 tcggttagcg cactacatca aggtgttgca gcgtgaacaa ctggggtcat ggaaagaaag
     8581 gagcgactta gaaagagagc ttaatacctg gatccgtcag tatgttgccg accaggaaaa
     8641 cccgcctgca gacgtgcgca gccgcaaacc tctgcgcgct gccaaagttg aggttatgga
     8701 tgtagaaggc gaacccggct ggtactaggt tgcgctaagc gtgagacctc attttaaatt
     8761 tatgggggca aattttgagc tttccctggt tggccggtta gacagggagt aacacaccat
     8821 gatactggat cgggaaagaa ccacaggttc tctttttgag cgtatggaag cctcttcggc
     8881 cagaaaccgg cagggaggca gtattcattc gctcaggcaa tccattcgcc agaacctaag
     8941 aaacattctt aatacccggt ccggtagttg ccggggtgcg cctgagctgg gcattgatga
     9001 accggaaggg gctgaaaact tccgcgaatc catgagtcgg gccatcgagc aatgtataga
     9061 acgctatgaa ccccggatct cacacgctga agtgcaggca gtggtttcat ccgcctccag
     9121 cccgctggat atgacgtttc atattacagc ctgggtgaca tttaacgaaa cgcatgaggt
     9181 gcttgaattt gatatggcac cgaatggcag ccagcattac cgggtggact gacaaatgga
     9241 atttgaagaa cgctattttc gggaagaact cgattacctg cgccagctta gcaagctgct
     9301 ggcaacggaa aaaccccatc tggcccgctt cctggccgaa aaagatgcgg atccggatat
     9361 tgaacgcctg ctggaagggg tggcttttct taccggcaat ctccgccaga aaattgagga
     9421 tgaattccca gaactgacgc acgggcttat taagatgcta tggcctaatt acctgcgtcc
     9481 ggttccggca atgaccctta ttgaatatac gccggatatg gataagtctt ctgtaccggt
     9541 gttaatcccc cgtaatgagc agtttacaac caacgccggg gaaatcagag ttgatgaagt
     9601 gctgccctct gatgctaaaa aggaggagcc gcctccctgt accttcaccc tctgccggga
     9661 tatctggctg ctgcccgttc gcctggagca gattgaaaac cgcagtacga cccgtaatgg
     9721 tgttatcaac atcacctttt cggtcgcacc gggaacggac ttccgcacgc tggatctgaa
     9781 caaacttcgc ttctggctcg gcaatgacga caactatacc cgtgaccagc tttatttatg
     9841 gttctgcgaa tacttgcagg gtgccgacct gactgtgggt gaacagcata ttcgcctgcc
     9901 tgagtttatg ctaaaagctg tcggttttga gccgcaggat gccatgctgc cctggccgaa
     9961 aaacgtccac agcggctacc ggatccttca ggagtatttc tgttaccccg atgcgtttct
    10021 cttttttgat ctttgtggtt gtccggcttt gcctgacgga ttgcaggcgg aattctttac
    10081 cctgcaactg cgtttttcgc gccctttgcc cgtggacatc cggctgcgcc gcgattccct
    10141 gcgcctgtat tgcgcacctg ccattaattt atttatccac catgcagaag ccatcacgct
    10201 ggacaaccgg cgggcagact atccgctggt tcccagccgc cattacccac aacattacga
    10261 tgtattttcc gttaacagtg tggtgagcca ggtccaggat atgttcagga aaaaagatct
    10321 ggggcgtcct gtttcgacgc aggccgcgcg ccagtggcca gcctttgaaa gtttcagcca
    10381 tcagatggaa tacagccgga agcgggaggt ggtgtactgg catcaccgga ccaaaacatc
    10441 cctgttccat cgcggctttg atcataccct tgcctttata catgctgatg gcagttatcc
    10501 gtcagacgaa tctctgctca gtaatgaagt ggtttcggta tcgctgacct gtaccaaccg
    10561 tgagcttccg tcacaaattc gttccggcga tatcaccggc acaaccggta aaaatgcggc
    10621 tgttgcttcc tttcgcaaca ttacccgccc gacgcaacca ctctggccgg tcattgatgg
    10681 cagcctgcac tggtccctgc tctccgccat gaacctgaat tatctgtcat tactggatac
    10741 ggacgcgctg aagcaggtca tcgccaactt tgatcgccac gcaatccacc atccgcagac
    10801 ggcgcggctg tcacaacaaa agctggatgc cattgagcgt ctggagaccc gccccgttga
    10861 tcgcctgttt acgggtattc ccgtccgggg actggcctcc acgctgtatc tgcacccgga
    10921 gccgtttgtc tgtgaagggg aaatgtatct gctcggtacg gtgctttcgc attttctgtc
    10981 gctgtacgcc agcgttaact cattccacat gctgaccgtt gtgaacacag aaagccagga
    11041 gacatggaaa tggacggaaa gaatcgggca gcatcctctt atctgagtcc gggaaatccc
    11101 ccggcagata aagagcagaa tgatccgctg gcacaggttt tccataatgc ctgctcttac
    11161 aatttttttg cgatggcgga gctgttgcac cgcctggcaa agggtgaaaa ggggacgcca
    11221 gaattatccc tgcgcgacga tccggcacag gaaaccctgc gtttcagcgc cgatgccagc
    11281 cttgccttcc cgtgcagtga tatcagtgcg ctgaaacggg atacgtcagg ggcattcagg
    11341 atgaccacta cctttatggg gttacagggg agccagtcgc ctctccccgg ttattacctc
    11401 gatcacctgg cctggaaagc ggtacatgaa caaagtccgg taggtgattt tctggatatg
    11461 ttcagccacc gcctgaccca gtttgtctgg catatctggc gtaagtaccg ctatcacatc
    11521 agttttcgta acggcggtgt ggatgcgttc tcgcagcgca tgtactccct tgtcgggctg
    11581 ggccaccgcc agcttcgcga taagctggcg attaatcaca gcaaaatgct ggcgtattcc
    11641 gggatcctgg caaatccggg acgatccccg gaaattatct gtggcctggt gtcccactgc
    11701 tttgatttgt cagaagtgac gctgcaaaac tggcaacgtc gcaaggtgga tattgaaccg
    11761 gatcagcaaa acagcctggg gagctattct cttaaaaatg gcgaaaaact ggcgggaaga
    11821 tcggtactgg gcaactttgt tctgggtaca cgagtacccg atctcagcgg aaaattccag
    11881 ctcagtatta ccagcctgac ccgaaaacag ttcctctctt ttctgccgtc cggcgaaaac
    11941 ttcctgccac tgacgacgtt cgtgtccttc atcctgcgcg atcagcttgc ctgggactta
    12001 catctggggc tggcgccgga gcaggtgggc gcaatgcgcc tgggcgataa caaaagtgcg
    12061 ctgctgggct ggaccagctt cctcggcaca ccggaagaac gaccctcagt caccatcagg
    12121 gtacggtcgt aaattatgat aatcacggag cagaatatgc cggaagagaa actacagacg
    12181 ctttcattgc aggtcattaa cggcagtgag ctggaaagcg ggcgggcggc gcgctgtctg
    12241 ttcacacagc agggaaatgt cggccatgcc cccgaatgcc actggtcggt acaggatcgt
    12301 cagcagagca ttccggccca ggcttttacc gttatcctgc acgatggcac attttgtcta
    12361 cgcccgcaga cggcacaact gtggctgaat caggcaaaag tcacagcaac atcagacctg
    12421 atacagttgc gccagggcga tgagatccag atcggacggc tgatggtgag ggttcatctg
    12481 aaccggggag atattcccca ttacgatgag gaaatggcca ctcccgaaac catcgttacc
    12541 aatcgcgata tgctcacgga taccctgcta tcaacggagg gtgcgccaca ctatccggga
    12601 atgactcacc ggcaccagct tgcagacacc gtggtaaatg gtttttctgc cgatccactc
    12661 caggcacttc agtccgaaag cctgattacc acgggcgatc cgctttcagg cattgcggct
    12721 gtccggccat cggcaccgct gtccgatccg gcaagtaatg gggggatcaa tactccgttt
    12781 atggatctgc cgcccattta tgccagccct ggcgatcata atgatgacat ctctgcggca
    12841 gaaatggcgc aacgccacct tgcggtcacc cccttactgc gcggtcttgg cggctcgctt
    12901 accgtgagca attccgacga tgcggatgat tttctggagg aggccggacg aacgttacag
    12961 gccgcaataa aaggtctgct cgatttgcag cagcagcgta acagcctctc agacaaacat
    13021 ttgcgcccgc tggaagataa cccgctgcgc ctgaacatgg attacgccac cgcgctcgac
    13081 gtgatgtttg ccgaaggtaa aagcccggta catctggcgg ctcccgccgc cgtcagtgaa
    13141 agcctgcgca atgtccgcca ccacgaagaa gctaaccggg cggcgattgt ggagtcgctt
    13201 cgtgtcctgc tggatgcttt ctcaccacaa aatctgctgc gccgctttgt gcagtaccgc
    13261 cgcagccatg aactgcgcca gccgctggat gatgccggag catggcaaat gtacagccat
    13321 tattacgaag aactggcctc cgatcgccag caggggtttg agatgctgtt taacgaggtc
    13381 tacgcccagg tctatgaccg ggtgcttcgt gaaaaacagc gggagccgga agcatgaaca
    13441 ggaaggcgtt tctggcctgc gtgctgatgt gcgtcatatt aaccggctgc gaaacggcga
    13501 aaaaaatcag cgaggtgatc aaaaacccgg atatccaggt cgggagcctg aaggagcaac
    13561 cttcggaaat aaccgtcacc ctgctgactg aaccggacac caataccaat gcggagggtg
    13621 aatcagccgc cgtggatgtg caactggtgt acctgactga cgactcaaaa ttgcaggcag
    13681 ccgactatga ccagatagcc agtacgccgc taccggatgt gctgggtaaa aactatatcg
    13741 accaccagga tttcaacctg ttgccggaca ccatcaaaac cctgccgccg ataaagctgg
    13801 atgagaaaac gcagtttatt ggtgtcgtgg cctatttctc cgatgaccag gccactgaat
    13861 ggaaacaaat cgagacggtg gaaggaaccg gccaccatta ccgcctgctg gtgcatgtac
    13921 gtcaaagcag catcgaaatg aaaaaagagg atgaataatt atggcaacca cccgcaacaa
    13981 ggtgatgtgg caggaaggga tgctgatgcg cccacaccat ttccagcaac agcagcgtta
    14041 caacgactac ctggataacc agcgtttccg ggccatgaat gatttatcct ggggatttac
    14101 cgaactcacc ctcaacaatg aactgctggc gcagggtaag atcatgattg acagcgcgtc
    14161 aggcacactg cctgacggca ccatcttttc tatccccgac caggacgcac tgcccgatcc
    14221 gctgcacccg caaaattttc cggacgaaag aagccgcaat atttacctcg ctctgccggt
    14281 cgccagtgat gtgagaaatg aaatcagcga cgggcggcga atcgggcgtt accggctgaa
    14341 ttatgccgat gtccgggatc tgcattcaga agaaggcgac gcgcgaacac tgacgctggg
    14401 acaactgacg ccgcgcatta tgagcggtgc agaagatatg agtgcctata ttacgctccc
    14461 gctttgccgt atcagcgatc gccatgccga cggctccctg acgctggatg acgattttat
    14521 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
    14581 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
    14641 cggcatcgcg gatgtggcgg aatttatgat gttgcagttg cttaaccgta accagacccg
    14701 gtttacccat cgcgcccgcc gatcccagct ccacccggaa gatttctacc ttgatcttgc
    14761 cgagttgctg ggtgaactga tgacctttac agagccgtca cgcctgccct gcccgcttga
    14821 tgtgtatgat catcatgacc tgaccaaaac atttaaaaca ctgttaccgg aagtcaaacg
    14881 ggcgctgcat accgtactgt cgccaagagc ggtcaatctg ccgctgcatc tgcgtgacgg
    14941 catctggcag gccgatgttc atgactcgga actgctacag tctgccacct ttgtactggc
    15001 cgtggcagca aatatgcctg tcgatcagat ccagcgtcag tttatccagc agtcgaaaat
    15061 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atcccgggta ttcctctgcg
    15121 cgcactgatg gtggcccccc gccagcttcc ctaccattcc gggttcagct atttcgaact
    15181 cgacaagagc ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
    15241 atccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
    15301 atgaggggtc actgtctctg ccaccattta ccggatacga cgaaaaaagc ctgcgcgact
    15361 accatctcgc gcttcatggc aacagcctga atccgatgat cgacgcggcg acgccgctac
    15421 tcggtatggt aatgcgcctt tccaccatga acagccagac gatgccggag catctttttg
    15481 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggaacag ggctacgaac
    15541 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
    15601 gtaacggctg gtcgaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
    15661 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
    15721 aacgttacca ggatctgctg gaatttctct ggctctgttt ttctctggga tttcgcgggc
    15781 gctacaaagt ggcagtacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
    15841 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
    15901 cccaaggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
    15961 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
    16021 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
    16081 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
    16141 ctcagaatgt atgagccagc aggcagcgga aattaccgtc agccatgtgc ttattcagat
    16201 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    16261 agagttgcgc caggcgttga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    16321 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctggctgc tggcgtcggc
    16381 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccttgc tgcattcgcc
    16441 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    16501 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    16561 cggtaaaggc gcaggcacac tgacggacgc ctctgacacc ctgcttgccc gctacgccaa
    16621 aaacatgaca gaagacgccc gtaacggcag gctagcccca gtactgtgcc gcgaccacga
    16681 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccgg tggtggtggg
    16741 cgaagcaggc gtgggtaaaa gcgcgctgat tgaagggctg gcgctgcgca tcgtggcagg
    16801 ccaggtgccg gacaaactga aaaacaccga tatcatgacc cttgacctgg gcgcattgca
    16861 ggccggggca tcggtgaagg gtgagtttga aaaacgcttc aaggggctga tggcggaggt
    16921 catttcctct ccggtgccgg tcattctgtt tatcgacgaa gcgcataccc tgattggcgc
    16981 gggcaaccag cagggcgggc tggatatctc caacctgctg aaaccggcgc tggcgcgtgg
    17041 cgaactgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgagaaaga
    17101 cgccgccctg tcgcgccgct tccagttagt gaaggtcagc gaacccaacg ctgccgaagc
    17161 caccattatt ctgcgtggtc tgtcggcggt ctatgaacgg tctcacggcg tgctgattga
    17221 cgatgatgcc ttgcaggccg cagcaacgct gagcgaacgt tatctctccg ggcgtcagtt
    17281 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    17341 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    17401 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    17461 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcttggca
    17521 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    17581 agaggacgat gcggcgccgt tgccggacgc agatactgtg gaggatacgc agccagagtc
    17641 agagtcagag tcagagtcag agtcagaaca ggataatacc ggtgccgtac cggctgatga
    17701 gaccgacaga gaacagccgg aagagaccgc tgaaacagtt tccccggtac agcggctggc
    17761 ccagctcact gccgaactgg acgccctgca taacgaccgg ttgctggtct ccccgcacgt
    17821 cgataaaaaa cagattgcgg cggtgattgc cgaatggacc ggcgtgccgc tcaaccgcct
    17881 gtcgcagaat gaaatgtcgg tcatcaccga cctgccgaaa tggctgggcg acaccatcaa
    17941 aggccaggac ctggcgattg ccagcctgca taagcatcta ctgaccgcac gcgccgacct
    18001 gcgtcgtccg ggacgcccgc tcggtgcgtt cctgctcgct ggccccagcg gcgtgggtaa
    18061 aaccgaaacc gtcctgcaac tggcagagct gctctacggc ggtcgccagt acctgaccac
    18121 catcaatatg tccgagttcc aggagaaaca caccgtttcg cggctgattg gctcccctcc
    18181 gggctatgtc ggctatggcg aaggcggcgt gctgactgaa gccatccgcc agaaacccta
    18241 ctcggtggtg ctgctcgatg aagtggaaaa agcgcacccg gatgtcctca acctgttcta
    18301 ccaggcgttt gacaagggcg agatggccga cggcgaaggc cgcctgattg actgtaaaaa
    18361 catcgtcttc ttcctcacct ccaacctcgg ttaccaggtg attgtcgaac acgcggatga
    18421 cccggaaacc atgcaggaag ccctctatcc ggtgctggcg gacttcttta aacctgccct
    18481 gctggcgcga atggaggtgg tgccgtacct gccgctgtcg aaagagacgc tcgccaccat
    18541 tatcgccggg aaactggccc gtctggataa cgtgctgcgc agtcgctttg gtgcagaagt
    18601 ggtcattgaa ccggaagtga cggacgaaat catgagccgc gtcacccgcg cggaaaacgg
    18661 cgcgaggatg ctggaatctg tcatcgatgg cgacatgcta ccgccgctct cgctgctgct
    18721 gttgcagaaa atggcggcta acacggcgat tgcccggatt cggttgtcgg cagtggacgg
    18781 cgcatttacg gcagacgtgg aagatgctca gaacgacgag tccgtcacaa aggatgaaac
    18841 ggttttatga gcaggttgcg cgttttccgg cagacaggat ggtttatcgc gggcctgatg
    18901 acgggcttac ccgccactgc cgcgcccgca gaagcatcgt caatggccgg tgtggcggtc
    18961 gccgtcgcca ccacgacccc gccggatgcg acagccacgt tacaggccat gcagtcctgt
    19021 cggcgggagt ccgcggcgct ggagcggctt gactgctatg accacctgct ggcaccgctg
    19081 tccccgtcag ggtttgacgg ggcgctggtc aaagccggtt ttgtgggcga ggcctggacc
    19141 cgcgccacag agcaggaaaa acgccgcgag ggcaacacca cagaactgct ggtgacgcag
    19201 gtgccggggg agcgcccgac ggtggtgata accaccccgg ctatcggcca tgttccgccg
    19261 cgcccggtgc tgatgttcag ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg
    19321 caccctctgg atgtccatga tatcgccgtc actctgaatg ccgacagccg ggcattgcgc
    19381 agccactggt ttgtgcgcga gaacggcacc ctgctggagt ccagccgggg gctgtccggg
    19441 attgatgaaa ttaagcaact gttcggcgca aaaacgctga cagtggatac cggcgcagat
    19501 aacgccgcag gaaagctgac ctttaacatt gacgggctgg caagggcgat tgcccccctg
    19561 cgcgatgcct gccactgggc gggagaataa aaatatggca atggacttac gcgatccgaa
    19621 tgtctggata tcgcacctgc tggaaaacct gccggaagaa aaactggcat cggcgctgaa
    19681 agatgacaac ccggactggg agtatatcga cggcgaaatc gtcaagctgg ggtctctggc
    19741 ccacgctcag cttgatattc ccgaactaca acgcaggggg ctacagcttc tggcttctga
    19801 aagcaaagac ttcaggctac tggcacacct gctcagaacc ctgcaacatg ccggtgatcc
    19861 actgctggca ctgcacctgc taacgctata cgtggaacat tactggactg tggccgcgcc
    19921 gcagaatatg gcgcataaaa aacgctttgc cagccagatc attaaacgtt ttgaaacggg
    19981 tattgaaggc ttttcacaaa acgctgccac aacgcagcgc gatactctgc tgggtgagct
    20041 ggcgaaactg gcgcagtgct ggcagtcaca taacgtcccg gaactggcac aggctaccga
    20101 tgatcttttt gccctgtacc agcgtacgtt taatcgtgcg gctcctgctc cggtccccac
    20161 tccggcggcc tccggtagtt caccacaaac caccgtcacg tctgaaagcg gcgtgacgca
    20221 acccagtgct ccggctcccc aaatcgccat cgacagtcac gacgacaaag cctggcgcga
    20281 cacgctgtta aaagtggcgg ctattttatg tgaacgccag ccggactcgc cgcagggcta
    20341 tcgcctgcgc cgccatgccc tgtggcaatc catcaccagt acaccccagg cggaaagcga
    20401 tggacgtacc ccactggctg cggtctctgc cgatatggtg gcggattacc agtcccggct
    20461 tgccagcgcg gatatggcgc tgtggcaaca ggttgagaaa agcgtattgc tggctcctta
    20521 ctggctggac ggtcactgtc tttctgcaca gacggcactg cgtctgggtt acaaacaggt
    20581 ggcagacacc atccgcgatg aggtcatccg cttccttgag cgtctgcccc agcttaccgg
    20641 gctgctgttt aatgaccgca caccgtttct cagtgagcag acgaaacaat ggctggctgc
    20701 ttcgcccgac ggcaaagttg caccggttgc gcaaatcggt gaggaatcgc aggcagccag
    20761 agcctgtttt gctgggcagg gtctggaggc ggcgctgcga tatctggaca tgctacccga
    20821 aggcgatccc cgcgatcagt ttcaccgcca gtaccttgcc gcacagttga cggaggaggc
    20881 ggggctgata cagcttgcgc agcaacagta ccggatgttg ttgatgatag ggagtcagat
    20941 gatggtgtct gactgggagc catcattact tacgcagctt gaacaaaaat tcacggcaga
    21001 acaataaaag agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg
    21061 actcaaatca gccctgcgac cggcgatgcc ccggtttaaa gtctccgctt tctggctgct
    21121 gatactggcg tggatttttc tgcttgctga tactggcgtg gatttttctg ctggtatgga
    21181 tctggtggaa aggcccgacg tggacgctgt atgaagaaca gtggctcaaa ccactggcga
    21241 accgctggct ggcaacggcg gcgtggggga ttattgccct ggtgtggctt accgtccggg
    21301 taatgaagcg cctgcaactg cttgaaaaac agcaaaaaca gcagcgcgaa gaggccattg
    21361 atccgctcag tgtggagctg aacgcccagc agcgttacct tgaccgctgg ctgctgcgcc
    21421 tgcaacgcca tctcgacaac cgccgtttcc tgtggcagtt accgtggtat atggtcatcg
    21481 gcccggcggg cagtggcaaa acgacactgc tgcgcgaggg gtttccgtca gacattattt
    21541 atgccccgga gggcgcgcgc ggcacagaac aacgcctgta ccttacgccc catgtcggta
    21601 aacaggcggt gatctttgat atcgacggca cactctgcgc tcccgctgat gcggatatcc
    21661 tgcatcgccg tctgtgggaa catgccctcg gctggctgaa agaaaagcgc gcgcgccagc
    21721 cgctgaacgg gattattctg acactcgatt tacccgatct gctgaccgca gacaaacgcc
    21781 accgcgagca tctgttacag acgctgcgca gccgcttaca ggatatacgc cagcatcttc
    21841 actgccagtt accggtttac gtggtactga cccggcttga tttattacag ggttttgccg
    21901 ccctgttcca gtccctgaac agacaggatc gcgatgcgat tctgggcgtc acgttcaccc
    21961 gccgtgccca tgaaaatgat gactggcgaa cagagttgaa tgctttctgg cagacatggg
    22021 tggatcgaat gaatctggcg ttgccggatc tgatggtcac ccagactcac acccgcgcgt
    22081 ctttattcag tttttcccgc cagatgcagg gaagccgtga accgctggtg tcactgcttg
    22141 agggtctgct tgacggtgaa aatatgaacg tgatgctgcg tggtgtctat ctcacctctt
    22201 cgcttcagcg cgggcagatg gatgatatat tcacccagtc tgccgcccgc cagtaccgac
    22261 tgggcaataa cccactggcg tcctggcctc tggtggacac cgcgccttat ttcacccgca
    22321 gcttgttccc gcaggcatta ctcgcagagc ctaatctggc aacggagagc cgcgcctggc
    22381 tgatgcgttc ccgtcgccgc ctgacggttt tctctgccac cggcggcgtg gcggcactgc
    22441 tgctcatcac cggctggcat cactattaca acggtaacta tcagtccggc atcaccgtgc
    22501 ttaagcaggc caaagccttt atggatgtgc cgcctccaca gggggaagat gactacggca
    22561 acctgcaact gccgctgctg aacccggtac gcgatgccac actggcctat ggcgactggg
    22621 gcgaccgcag ccgtctggcc gatatgggac tgtaccaggg acgacgtatc ggaccttatg
    22681 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
    22741 tggttaaagc gatgaatgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
    22801 tgatgcgaat gctggaggac aaaagcggac gtaacaatga ggtggtgaag cagtatatgg
    22861 caaaacgctg gagcgaaaag ttccacggcc agcgcgatat ccaggcacaa ctgatgtccc
    22921 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
    22981 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
    23041 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
    23101 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
    23161 ctgccgatga caacaaactg gttgttccac agtttcttac ccgttacggc ctgcaaagct
    23221 attttgtaaa acagcgcgat gaactggttg aactgacggc gatggattcc tgggtactta
    23281 accttacccg cagcgtgaaa tacagtgacg ccgaccgcgc ggaaatccag cgccagctca
    23341 ccgagcagta tatcagcgac tacaccgcca cctggcgggc cgggatggac aatctgaata
    23401 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
    23461 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacaa cacacagcca ggcgtctttt
    23521 ctgaaaaact ctctgccaaa gaacgggatg aggccctggc agagccggac taccagttac
    23581 tcacccgtct cgggcatgag ttcgccccgg aaaacattac cctggcagta cagaaagaca
    23641 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
    23701 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agcagtgcag ttacggcttg
    23761 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
    23821 cgctcaaccg ctgggttggc agactgactg accaggcctg gcatgtggtg atggtggagg
    23881 ctgtacatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
    23941 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
    24001 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
    24061 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
    24121 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
    24181 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
    24241 tactgaacct tgacggtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
    24301 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
    24361 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttcg
    24421 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
    24481 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
    24541 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
    24601 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
    24661 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
    24721 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
    24781 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
    24841 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
    24901 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
    24961 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
    25021 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
    25081 tttatgcaga acacgcgtaa acggcttgaa ccccagctga agagtaacac tgagaacgcc
    25141 gcacagacca cggtcagaac gctgatttat gccccggaaa cacaggcatc ttccacacca
    25201 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
    25261 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
    25321 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
    25381 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
    25441 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
    25501 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
    25561 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
    25621 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
    25681 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
    25741 caggcggagc ggggactggg gcaggaaaca cccgttgagt ctctgttgac gcagtaccag
    25801 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
    25861 gaaggcgtgt taagccgctg gctgctgctg aagaataata cgataccgac gataaaaaaa
    25921 gcattgaatt tcaataacat tcatgaatac aaaggagttt taaatggcga atttaattta
    25981 tttaacacta aatggtgata atcaagggct catttcaagt ggttgttcat cacaaccatc
    26041 cattggaaat aaagctcaaa cagcacacat tgatcaaatt atgatttatg aattcatgca
    26101 tggattaaat cgagatcaaa acgtcaacca tcatcatttg acaataaaaa aacctataga
    26161 taaagcatca cccttattag gcaaagcaat ttgcgacaat gaattattga catgtgattt
    26221 ttccttttat cgtacaaata aatttggaat taatgaactg ttctataaaa tcaaattaac
    26281 tggagcaaag ataagcgaca ttcatgtctc tatatcacat attgttgttg ataacagcgt
    26341 tcaacccgaa gagtcagttt ctttcagtta tgagtcaatt atatgggagc attgttccgc
    26401 tggcactagt gcatacagtt tatgggaaga tcgtcttttt taaaaacata tgggattatt
    26461 ataatggcta acaatatttt acactcttca acatcagaca gtgaaagcaa aaatacaagc
    26521 actgacagta cgttatttaa tgaaaaattc ccatttcaac taagacaaga tttcgcatca
    26581 agaataacag aagatgaaag tgattttttt aaatggaaaa aaataagaga gttggcattt
    26641 gtgagcaatc ataaagaatg gaataaatat gacttattaa tttaaaaatc tgttaatgaa
    26701 atcaacattc atttgtccag cactccatac tttcagccgt tggattggta tataataaaa
    26761 gccatgctct ggactgaaaa cgatgcggaa aatacttctc agtggaatgg ttatccatta
    26821 caaattggtc gttttcggaa agataaagcg atgccagctt taatctctgg agaaaaatct
    26881 acagcccttg ttaccccacc acaatggaga aacaaagcat tcaatgggtt aaaagatcca
    26941 gaaagaaatt actgggcaaa agaacaaata actggctctc cagaagagaa tatcaaagca
    27001 gcaattactt acttaatgat gaaactttct aatacaaagg aagaatcaac tatcgatcaa
    27061 tatgactcaa ctctatatag cgccattgtc caaaaaggcg atttggctga caacatcaga
    27121 aaagagagaa aaacgaccat acctaactta acgaaaaaca atcctggaaa aaatctcgat
    27181 aaaatacacc cgggagatat tttatattat caaaaagcat ccatgaaagt aataattact
    27241 ggatggaaac ctatcacaat aaaaaatgta gcgatgaatt ataatggtgg tggcgatccc
    27301 aagtatgcaa taaaacttca gttcgtttat acattactca caaaaaacag agtattatga
    27361 gaatgcgaaa aataacaatt attctactgc tgacattctc atgtacaaaa ggtctggcat
    27421 ttgacatttc catgtctaaa gaatgtagtg gtgttgatga acttgaattt attgattgtg
    27481 ttaaaaactc
//
LOCUS       DAZFBR010000066        27364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFBR010000066 DAZFBR010000000
VERSION     DAZFBR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.066"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            118..660
                     /gene="zapC"
                     /locus_tag="ACHJZ2_002937"
     CDS             118..660
                     /gene="zapC"
                     /locus_tag="ACHJZ2_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO4569011.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(657..1766)
                     /gene="ycbX"
                     /locus_tag="ACHJZ2_002938"
     CDS             complement(657..1766)
                     /gene="ycbX"
                     /locus_tag="ACHJZ2_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO4569012.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            2010..4118
                     /gene="rlmKL"
                     /locus_tag="ACHJZ2_002939"
     CDS             2010..4118
                     /gene="rlmKL"
                     /locus_tag="ACHJZ2_002939"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO4569013.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4130..6037
                     /gene="uup"
                     /locus_tag="ACHJZ2_002940"
     CDS             4130..6037
                     /gene="uup"
                     /locus_tag="ACHJZ2_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4569014.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6167..7420
                     /gene="pqiA"
                     /locus_tag="ACHJZ2_002941"
     CDS             6167..7420
                     /gene="pqiA"
                     /locus_tag="ACHJZ2_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO4569015.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7425..9065
                     /gene="pqiB"
                     /locus_tag="ACHJZ2_002942"
     CDS             7425..9065
                     /gene="pqiB"
                     /locus_tag="ACHJZ2_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO4569016.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9062..9625
                     /gene="pqiC"
                     /locus_tag="ACHJZ2_002943"
     CDS             9062..9625
                     /gene="pqiC"
                     /locus_tag="ACHJZ2_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO4569017.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9881..10048
                     /gene="rmf"
                     /locus_tag="ACHJZ2_002944"
     CDS             9881..10048
                     /gene="rmf"
                     /locus_tag="ACHJZ2_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO4569018.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10118..10636)
                     /gene="fabA"
                     /locus_tag="ACHJZ2_002945"
     CDS             complement(10118..10636)
                     /gene="fabA"
                     /locus_tag="ACHJZ2_002945"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO4569019.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10705..12465)
                     /gene="ycbZ"
                     /locus_tag="ACHJZ2_002946"
     CDS             complement(10705..12465)
                     /gene="ycbZ"
                     /locus_tag="ACHJZ2_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4569020.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12651..13103
                     /gene="matP"
                     /locus_tag="ACHJZ2_002947"
     CDS             12651..13103
                     /gene="matP"
                     /locus_tag="ACHJZ2_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO4569021.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13179..14219)
                     /gene="ompA"
                     /locus_tag="ACHJZ2_002948"
     CDS             complement(13179..14219)
                     /gene="ompA"
                     /locus_tag="ACHJZ2_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO4569022.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14576..15085)
                     /gene="sulA"
                     /locus_tag="ACHJZ2_002949"
     CDS             complement(14576..15085)
                     /gene="sulA"
                     /locus_tag="ACHJZ2_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO4569023.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15304..15933
                     /gene="sxy"
                     /locus_tag="ACHJZ2_002950"
     CDS             15304..15933
                     /gene="sxy"
                     /locus_tag="ACHJZ2_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO4569024.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15896..18058)
                     /gene="yccS"
                     /locus_tag="ACHJZ2_002951"
     CDS             complement(15896..18058)
                     /gene="yccS"
                     /locus_tag="ACHJZ2_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO4569025.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18068..18514)
                     /gene="yccF"
                     /locus_tag="ACHJZ2_002952"
     CDS             complement(18068..18514)
                     /gene="yccF"
                     /locus_tag="ACHJZ2_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO4569026.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18637..20691
                     /gene="helD"
                     /locus_tag="ACHJZ2_002953"
     CDS             18637..20691
                     /gene="helD"
                     /locus_tag="ACHJZ2_002953"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO4569027.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20723..21181)
                     /gene="mgsA"
                     /locus_tag="ACHJZ2_002954"
     CDS             complement(20723..21181)
                     /gene="mgsA"
                     /locus_tag="ACHJZ2_002954"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO4569028.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21277..21939)
                     /gene="yccT"
                     /locus_tag="ACHJZ2_002955"
     CDS             complement(21277..21939)
                     /gene="yccT"
                     /locus_tag="ACHJZ2_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO4569029.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22112..22525
                     /gene="yccU"
                     /locus_tag="ACHJZ2_002956"
     CDS             22112..22525
                     /gene="yccU"
                     /locus_tag="ACHJZ2_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO4569030.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22570..22887)
                     /gene="hspQ"
                     /locus_tag="ACHJZ2_002957"
     CDS             complement(22570..22887)
                     /gene="hspQ"
                     /locus_tag="ACHJZ2_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO4569031.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22945..24135)
                     /gene="rlmI"
                     /locus_tag="ACHJZ2_002958"
     CDS             complement(22945..24135)
                     /gene="rlmI"
                     /locus_tag="ACHJZ2_002958"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO4569032.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24230..24508
                     /gene="yccX"
                     /locus_tag="ACHJZ2_002959"
     CDS             24230..24508
                     /gene="yccX"
                     /locus_tag="ACHJZ2_002959"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO4569033.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24505..24834)
                     /gene="tusE"
                     /locus_tag="ACHJZ2_002960"
     CDS             complement(24505..24834)
                     /gene="tusE"
                     /locus_tag="ACHJZ2_002960"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO4569034.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24925..25584)
                     /gene="yccA"
                     /locus_tag="ACHJZ2_002961"
     CDS             complement(24925..25584)
                     /gene="yccA"
                     /locus_tag="ACHJZ2_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO4569035.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25992..27011)
                     /locus_tag="ACHJZ2_002962"
     CDS             complement(25992..27011)
                     /locus_tag="ACHJZ2_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4569036.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26989..27231)
                     /locus_tag="ACHJZ2_002963"
     CDS             complement(26989..27231)
                     /locus_tag="ACHJZ2_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO4569037.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
BASE COUNT         6944 a         6655 c         7144 g         6621 t
ORIGIN      
        1 tgttttatat tcattgctgt tgcttattta gacattttgt acttttattg ttgaggttat
       61 taagcgaagc gacaatggat tgtgttgctg cggttttata gggtagggga gaggcagatg
      121 cgaattaaac cagacgataa ctggcgttgg tattacgatg aagagcacga tcgtatgatg
      181 ctcgatttag ccaatggtat gctatttcgc tcacgttttg cgcgcaaaat gttgactcca
      241 gatgcctttt ctcccgcagg cttttgcgtt gacgacgccg cgctctattt ctcttttgaa
      301 gaaaagtgcc gcgattttaa tttatctaaa gaacaaaaag ccgaactggt gctgaatgca
      361 ctggtagcaa ttcgctatct caaaccgcaa atgccgaaaa gctggcattt tgtttcccat
      421 ggtgaaatgt gggtgcccat gccgggcgat gcggcctgcg tttggttgag cgacactaac
      481 gagcaagtga atttactggt cgttgaatct ggcgagaatg ccgcgctatg cctgctggca
      541 caaccctgcg ttgttattgc gggtcgcgcg atgcagttgg gcgacgccat taaaatcatg
      601 aacgacaggc tgaaaccgca ggttaatgtt gacagcttca gcctcgaaca ggcagtctaa
      661 cgcgccaact taagtgcagt cttcggtaca cagctacagc aaagaatggt gccatcatcg
      721 cccattgctg attttttcag cggcgtgact tcgccttcta aaagctgaac acggcaactt
      781 ccacaaatgc ccgcgcggca agaataaggg atacgaattc cctgattttc taattgctcc
      841 agcaacacct gttggttatt tccacgaaat gcctgtccct gccagtcaat atctacattg
      901 gcgtccggtt gttgcgtgat gttggcagta tcatcagcgg cagctgcgcc gtaaattttt
      961 gccggagccg ttgtcagaat ttccacctca tcgccaacgc gaatcacgcc gctattacgg
     1021 gcaattaaat tctgaccaaa atcgacatcg ccgttatcct gggcagtgcg gaaagattgc
     1081 aatgttttta atggttcgcc tgccggatgt ttttgccctt tttctgggct gacggtggtg
     1141 aaaatacagc ggctacaagg tttaaccaca tcaaacacca catcaccaat gcgaatcact
     1201 ttccaactat cttcttccca ggctgacgcg ccggaaacca ccagattggg gcggaattgc
     1261 tccattttta cactggccgg acaacgttgt tggagatcac gtaacgaggc ttcgttagca
     1321 agaaggtaag gatagccatc agcaaatgac aggggtacag tgttgtggcg tttcacgcgc
     1381 cgggtcattt gtggccccac ccagcgtaat tgcacttcgc gggagaaaaa tccacttaac
     1441 catttgttga tcgcttctgg cgcaattcgc gcggtaaaat gtgtgcccca aacttcggtt
     1501 ggtgcgtctt gtgtggcgaa atcagcaaaa cgaacatatg cgctactgct atctggtgcg
     1561 gtgagatgca agccatcatg cacgggtgaa ggagtaaatc gtaccatctg gggaaactgg
     1621 cgagcggtga taaacgtacc gtcaggttcc gtgatcataa agatgcgatc gaaggccaga
     1681 ccactgacat ctgccagagc atgtgtaaga ccaatgccgc gcatcgattt aacaggatga
     1741 ataaaaagcc gggttaatgt cgccacggtg cggtcctcaa atgaaaataa gccctcaact
     1801 ttatgacatg acgcgcttat tagctataat gcgcaacaat tttcttagcg cctgtcccat
     1861 taggctattt tatttgtcag tttggccctg ggttgtgctc gaaatcctca cgtacttgtg
     1921 tacgctccgg tttctccgcg ctgtccatgt ccagactgac tgcaacaatt acgcctactg
     1981 cggtaggctc ttagagtaaa agtgacgata tgaattctct gtttgccagt acggcccgtg
     2041 ggctggaaga gctgttaaaa actgaactgg aaaacctggg ggccgttgaa tgccaggtgg
     2101 ttcagggtgg ggtccatttc aagggcgaca cacggcttgt ttaccagagc ctgatgtgga
     2161 gccgcctggc ctcgcgtatt atgttgccgc tgggcgagtg taaggtttac agcgatttag
     2221 acctctatct cggtgttcag gcgatcaact ggacagagat gtttaatcct ggcgcgacct
     2281 tcgctgtcca cttcagtggt ttgaatgaca ctatccgcaa cagtcagtac ggcgcgatga
     2341 aagtgaaaga cgcgatcgtc gatgctttca cgcggaaaaa tctgccgcgt ccaaatgttg
     2401 atcgcgatgc gccggatatc cgcgttaacg tctggctgca taaagaaacc gccagtatcg
     2461 cccttgatct cagtggtgat ggtttacatc tgcgtggcta tcgcgatcgt gctggtattg
     2521 cgccgatcaa agaaaccctg gcagccgcta ttgtgatgcg atccggctgg cagccaggaa
     2581 caccgttgct cgatccgatg tgtggttccg gtacgttgct gattgaagcg gcgatgctgg
     2641 cgaccgatcg cgcaccaggc ttgcaccgtg ggcgttgggg ctttagcggc tgggcgcagc
     2701 atgatgaagc tatctggcag gaagtgaaag cggaagcgca aactcgcgcc cgtaaaggcc
     2761 tggctgagta tagctctcat ttttacggtt cggacagcga cgctcgggtg attcaacgtg
     2821 cacgcactaa cgcccgtctt gcggggattg gtgaactgat cacctttgag gtgaaagatg
     2881 tcgcgcaact gaccaatccg ctgccgaaag agccgtacgg cacagtgtta agcaacccgc
     2941 catacgggga acgtctggac agcgaaccgg cgctgattgc gctgcatagc ctgctgggtc
     3001 ggatcatgaa aaaccagttt ggtggctgga atctctcttt gtttagtgcc tcgccggatc
     3061 tgctaagctg cttgcagctg cgtgcagaca aacagtacaa ggcgaaaaac ggcccgctgg
     3121 actgcgtaca gaaaaattac cacgttgccg aaagcactcc agacagcaaa ccggcgatgg
     3181 tagcggaaga ctacgccaac cgcctgcgta agaacctcaa aaaattcgag aagtgggctc
     3241 gtcaggaagg gattgaatgt taccgcctgt atgacgccga tctgccggaa tataacgttg
     3301 ccgttgaccg ttatgccgac tgggtggtgg tgcaggagta tgcgccgcca aaaactattg
     3361 atgcgcacaa agcgcgtcag cgtctgttcg atattatcgc tgcaaccatt tcggtactgg
     3421 ggattgcgcc aaacaaactg gtgctgaaaa cccgtgaacg tcagaagggc aaaaatcagt
     3481 accagaaact gggcgagaag ggcgagtttc tggaagttac cgaatataac gctcacttgt
     3541 gggtgaacct gacggattat ctcgatactg gtctgttcct cgatcaccgt atcgcccgtc
     3601 gtatgctcgg ccagatgagc aaaggcaaag atttcctcaa cctgttctct tataccggca
     3661 gcgccaccgt gcatgcggga ttaggcggtg cacgcagcac caccaccgtg gatatgtcgc
     3721 gtacttatct ggagtgggca gaacgcaacc tgcgtctgaa tggcctaacc gggcgtgcgc
     3781 atcgcctgat tcaggccgat tgcctggcgt ggctgcgtga ggcaaatgaa cagttcgatc
     3841 tgatctttat cgatccgcca accttctcta actcaaaacg aatggaagat gcgtttgatg
     3901 ttcagcgcga tcatctggcg ctgatgaaag atttgaaacg tctgctgcgt gcaggtggga
     3961 cgatcatgtt ctcgaacaac aaacgtggct tccgtatgga tctcgacggc ctggcaaaac
     4021 tgggactgaa agcacaagaa attacgcaaa aaacgctctc ccaggatttc gcccgtaacc
     4081 gccagatcca caactgctgg ctgattaccg cagcctgaaa ggaatagtaa tgtcattaat
     4141 cagtatgcat ggcgcatggc tgtcgttcag cgacgcgccg cttctcgata acgcagaact
     4201 gcatatcgaa gataacgaac gtgtttgtct ggtgggccgc aacggcgcag gcaaatcgac
     4261 gttaatgaaa atcctcaacc gtgaacaagg gctggatgac ggtcgcatta tttacgagca
     4321 ggatttgatt gtagcgcgtc tgcaacagga tccgccgcgt aacgttgagg gtagtgttta
     4381 tgatttcgtt gccgaaggca ttgaagaaca agcggaatac ctgaaacgct atcacgatat
     4441 ttcgcgcctg gtgatgaacg acccgagcga gaaaaatctc aacgaactgg cgaaggttca
     4501 ggaacagctg gatcaccaca acctgtggca gctggaaaac cgcatcaacg aagtgctggc
     4561 gcaactgggg ttagatccta acgttgcgct gtcgtcgctt tccggcggct ggttgcgtaa
     4621 agcggcatta ggacgcgcgc tggtgagcaa tccgcgcgta ttgctgctgg acgaaccgac
     4681 taaccacctg gatatcgaaa ccatcgactg gctggaaggg ttcctcaaaa ccttcaatgg
     4741 gacgattatt ttcatctccc acgaccgttc gtttatccgc aatatggcga cgcgcattgt
     4801 tgatctcgat cgcggcaagc tggtgaccta tccgggcaat tacgatcagt acctgctgga
     4861 aaaagaagaa gccctgcgcg tggaagaact acaaaatgcc gagtttgatc gcaaactggc
     4921 gcaggaagaa gtgtggattc gccaggggat caaagcacgc cgtacccgta atgaaggccg
     4981 cgtacgcgcc ctgaaagcga tgcgtcgcga gcgtggcgaa cgtcgcgaag tgatgggtac
     5041 cgcaaagatg caggtggaag aggccagccg ctccggtaag atcgttttcg aaatggaaga
     5101 cgtttgctac caggttgacg gaaagcaact ggtgaaagat ttttctgccc aggttctacg
     5161 tggcgacaaa attgccctga ttgggccgaa tggctgcggc aaaaccacgc tgctgaaact
     5221 gatgctcggt cagcttcaag cggacagcgg gcgtattcac gttggcacca aactggaagt
     5281 ggcttatttc gatcagcacc gcgcggaact ggatcccgat aaaacggtga tggataacct
     5341 tgccgaaggt aagcaagagg tgatggttaa cggcaagcca cgccacgttt tgggctattt
     5401 gcaggacttc ctgttccatc cgaaacgggc gatgacgcca gtacgtgcgc tttctggcgg
     5461 tgagcggaac cgcttgctgc tggcgcgttt gttcctcaaa ccaagcaact tattgattct
     5521 tgacgaaccg accaacgatc ttgatgtcga aacgctggaa ctgctggaag aactgatcga
     5581 cagctaccag ggcacggtat tgctggtaag ccacgatcgt cagtttgtcg ataacaccgt
     5641 tacagaatgc tggatcttcg aaggcggcgg taaaattggt cgttatgtcg gcggttatca
     5701 tgatgcccgt ggtcagcaag agcagtatgt ggcgctcaaa cagcctgcgg tgaaaaaaaa
     5761 cgaagaaccc gccgcgccaa aagcagaaac tgtaaaacgc agcagtagca aactaagcta
     5821 taaattgcag cgcgaactgg agcagctacc gcaattgctc gaagatctgg aggcgaagct
     5881 ggaagcccta cagacgcagg tggcggatgc ttccttcttc agtcagccgc atgagcagac
     5941 acaaaaagtg ctcgccgata tggctgctgc agagcaggag ctggagcaag cctttgaacg
     6001 ctgggagtat cttgaagcgt taaaaaatgg tggctgatcg caaaatagtc gatttaaaca
     6061 gagacagctg acaacggtaa aattgcctga tgaactgggt ttatcaggca tgaaaacaaa
     6121 cactttgtta gtcatctgta aaatagcgca tcattaagga gtaccaatgt gcgaacatca
     6181 tcatgccgcg aagcacatcc tgtgctcgca gtgtgacatg ctggtggcgt taccgcgcct
     6241 tgagcatggt cagaaagcgg catgtccccg gtgtggcaca acgttaaccg tggcgtggga
     6301 tgccccccgg cagcgtccga ccgcctatgc gttggctgca ctgttcatgc tgttgctgtc
     6361 caacttgttt ccttttgtga atatgaacgt tgcaggagtt accagtgaaa ttacattact
     6421 ggaaattccc ggcgtgcttt tttctgagga ctacgccagc ctcggcacct ttttcctgtt
     6481 gtttgtgcaa ctggttcccg cgttttgtct gataaccatt ctgttactgg tgaatcgcgc
     6541 ggaattaccg gtccgtttaa aagagcaact ggcacgggtg ctttttcaac tcaaaacctg
     6601 gggaatggcg gagattttcc tcgcgggtgt gctggtcagt ttcgttaaac tgatggctta
     6661 cggcagcatt ggcgtaggca gcagctttct cccctggtgt ttattttgtg tcctgcaact
     6721 gcgcgctttt cagtgcgttg atcgtcgctg gttatgggac gatatcgccc cgatgccaga
     6781 actgcgccag ccgctaaaac caggcgtcac ggggatacgt caggggctgc gttcgtgctc
     6841 ctgttgtacg gcaatccttc ctgctgatga acccgtgtgc ccgcgctgtg gtactaaagg
     6901 gtacgttcga cgtagaaaca gcctgcaatg gacactcgcg ctgcttgtta cgtccatcat
     6961 gctgtatctt ccggctaata ttttgcccat catggtgacg gatttattag gctcgaagat
     7021 gccatcgacg attctcgctg gggtcatcct gttatggagc gaaggctctt atcccgtcgc
     7081 tgcggtgatc tttctggcca gtattatggt gccaacgtta aagatgatcg ccatcgcgtg
     7141 gctgtgttgg gatgccaaag ggcatggcaa gcgcgacagt gaaagaatgc atttgattta
     7201 tgaagttgtt gagtttgtag gccgctggtc gatgattgac gttttcgtta tcgcggtgct
     7261 ctcggcgctg gtgcgtatgg gaggtttaat gagtatttat ccggcaatgg gtgcattaat
     7321 gtttgcttta gtcgtcataa tgacaatgtt ttctgctatg acgtttgacc cgcgtttgtc
     7381 gtgggatcgt caacctgaat cagagcatga ggagtcctga cagtatggaa tctaataatg
     7441 gggaagccaa aatccagaaa gtgaagaact ggtctcccgt gtggatattt cctatcgtca
     7501 cggcgctcat tggggcctgg gttctttttt atcattacag ccatcaggga ccggaagtga
     7561 ccctgatcac cgcgaatgcg gaaggaattg aaggtggcaa aaccaccatt aaaagccgta
     7621 gcgttgacgt cggcgtggtt gaaagcgcca cactggctga tgatttgaca cacgttgaaa
     7681 tcaaagcgcg gctgaattcc ggtatggaaa aattgctgca taaagatacc gtcttttggg
     7741 tggtgaaacc gcagattggt cgagaaggga ttagcggtct gggaaccctg ctgtctgggg
     7801 tttatatcga actgcagcca ggcgcgaaag gcagcaaaat ggataaatac gatttgctgg
     7861 actcgccacc gttggccccg cctgatgcga aaggtatccg tgtggttctc gatagcaaaa
     7921 aagccgggca gctctcgcca ggagatccgg tgctgttccg tggctatcgg gtaggttcgg
     7981 ttgaaaccag caccttcgat acgcaaaaac gcaatatcag ttatcaactg ttcatcaatg
     8041 caccttatga ccgactggtg accagcaatg ttcgcttctg gaaagatagt ggcattgcgg
     8101 ttgatctgac gtcagcggga atgcgtgtgg agatgggctc attgacaacg ctgctgagtg
     8161 gcggtgtcag ctttgatgtg ccggaaggtc tggatttagg gcagccagtg gcaccgaaaa
     8221 cagctttcgt tttgtatgat gatcagaaga gcattcagga ttcgttgtac accgatcaca
     8281 ttgattatct gatgttcttt aaagattcgg tacgcggtct gcaaccggga gctccggtag
     8341 aattccgggg tattcgcctc ggtaccgtaa gcaaagtgcc attctttgcg ccgaatatgc
     8401 gtcagacatt taacgatgat taccgtattc cggtgctgat tcgtatcgag ccagagcggc
     8461 tgaaaatgca gcttggcgaa aatgcggatg ttgttgagca ccttggcgaa ttgttgaaac
     8521 gtggtttacg cggatcgctg aaaaccggaa acctggtcac tggcgcactg tatgttgatc
     8581 tcgatttcta tccaaatacg cctgcaataa ccggtattcg tgaatttaat ggttatcaga
     8641 ttatcccgac cgttagcggc ggcctggcgc aaatccagca acgactgatg gaagcgttgg
     8701 ataagatcaa caaactgcca ttgaatccga tgattgaaca ggcaaccagt acgctttctg
     8761 aaagtcagcg cacaatgaaa aacctgcaaa cgacgctgga tagcatgaac aagatcctcg
     8821 ccagccagtc gatgcagcag ttgccgacgg atatgcagtc aacgttgcgt gaattgaatc
     8881 gcagcatgca gggcttccag cccggctccg cagcctacaa caagatggtg gcggatatgc
     8941 agcgccttga tcaggtgttg cgagaactgc aaccggtgct gaaaacgctc aatgagaaga
     9001 gtaacgcgct ggtatttgaa gcgaaggaca aaaaagatcc agagccgaag agggcgaaac
     9061 aatgaaaaag tggctagtga cgattgcagc actgtggctg gccggatgca gctccggcga
     9121 aattaataaa aactattacc agttacctgt ggtgcagagc ggtacacaaa gtaccgccag
     9181 ccagggcaat cgcctgttat gggtagagca ggtcgctgta cctgactatc tggcggggaa
     9241 tggtgtggtt tatcaaacca gtgatgtgaa gtatgtgatt gccaacaaca acttgtgggc
     9301 cagcccgttg gatcaacagt tgcgcaacac cctggtagcc aacctgagca cgcaactgcc
     9361 cggctgggtg gttgcctccc agcctctggg aagcgcccag gacacgctca atgttaccgt
     9421 aacggagttt aacggtcgct atgatggcaa ggtcattgtc agtggtgagt ggctgttgaa
     9481 ccaccaggga caactgatca aacgtccgtt ccgtctggaa ggagtgcaaa ctcaggatgg
     9541 ttacgatgag atggttaaag tgctggccgg tgtctggagt caggaagccg cttctattgc
     9601 acaagagata aagcgtctac cttaattata aagatttgta aatataaccg tctccggtat
     9661 gttgcctgag gcggtttttt tgtttctaac gtgcggaaaa atttgttcct cttcacattt
     9721 tttgtacaac cgacatgccc gtgtagctca caaatatgac agtggcgtga attttgcgca
     9781 ttgacggcag ttatgattcg cggtattgct taactgtgat tgcacattta gtaatcactg
     9841 ttttcttttc caccagaaac cagtatgagg gaaacgaggc atgaagagac aaaaacgaga
     9901 tcgcctggaa cgggcacatc aacgtggtta tcaggccggc atcgccggac gctcaaaaga
     9961 aatgtgtccc tatcagacgc tgaatcaaag gtcacaatgg ctgggaggct ggcgagaagc
    10021 catggcggac agggtagtaa tggcctgatt ctgtctcttt aaaaagaaac ctccgcattg
    10081 cggaggtttc gccttttgat actctgtctg attataatca gaaggcagac gtatcctgga
    10141 acagaccgac tttcaggtcg ctggcggtat agatcagacg accatcaacc agcacttcgc
    10201 catccgccag gcccataatc agacgacggt taacaatgcg tttaaagtga atacggtagg
    10261 tcactttttt cgctgtcggc agtacctgac cagtgaattt cacttcgcca acgcccagcg
    10321 cgcggccttt accttcgccg cccagccagc cgaggtagaa ccctaccagc tgccacattg
    10381 cgtccaggcc caggcatccc ggcataaccg gatcgccaat aaagtggcat ccgaagaacc
    10441 acagatccgg attgatatcc agttctgctt caacataccc tttgtcgaag ttaccacccg
    10501 tttcggtcat tttgaccaca cggtccatca tcagcatgtt cggtgctggc aattgcgggc
    10561 ctttagcgcc aaacagttca ccgcgaccag aggcaagaag gtcttctttt gtataggatt
    10621 cgcgtttatc taccatgttc tctgtaagcc ttattttatt gaagcacgca ggatagctaa
    10681 cacgtgtacg ctgaacaagt ccgatcagtt cggaataaac cagttcagcc aacgtaatgg
    10741 ccatggaaaa cggtgacgtc cttcctgttg cgatgcttgc gcgatacgtt cctggatggt
    10801 ttgcatcagc gtcgtttggc cttcgccatc ccacaccaga tttaataata acggcagtgc
    10861 gtcagtcaca tcgtctactg cccagatggt gaatttgcct tcttctaccg ctttcaccag
    10921 ttcactgtga agacttaaat ggcgaacgtt agctgtgggg ataatgacac cttgtttccc
    10981 ggttaactca cgttgctggc aaatagcaaa gaagccttcg attttctcat ttaaaccacc
    11041 gaccggctgg gcgcgaccga actgatcgac tgaacctgtg atagcgatac tctgattcac
    11101 cggcacatcg gcgagggcgc ttatcagggc gcagagttca gccatcgagg cactatctcc
    11161 atcaacttca ctgtatgact gctcaaatgt cagcgatgct gagaagggga tctgttgctc
    11221 aagctgtagt tccgacatca ggaacgcttg catgatcatc atccctttcg catggatatt
    11281 gccgccaagc tccgctttgc gttcgatgtc ggtgaattca ccatcgccaa tatgcacaac
    11341 gcagctaatg cgagaaggtt cgccaaaagc gcgtggatga cccggaaatt caatgaccga
    11401 aagggcgtta atttgcccga tgcgttcgcc ttcggtttca atcaggattt gctcctgaag
    11461 gatctcatcc tgcatacgtt cagcgaggaa accttcgcgc cattcacgct gctgcagcat
    11521 taagtttagc tgctcgccgg agaaggtgtc gccatcacac agggaggcga cctctttaca
    11581 ctggcggagg atccactgcg ggctaagcgg aagtgtttct tgttcaccgg tgtagcgtgc
    11641 tgcttcgcgg ataagtaccg gccaggcatc cgctcccggg gcaggtaagt gattatgtct
    11701 ggcggtaaat gtcacccagc gacaccactg ggttactgac tccgcatcga caatctgcag
    11761 agtatcttca aattcgctat aaatagcctg ctctgaaagc tctggctcca tctcctggaa
    11821 atcagccaat gattcgcgtt cgcctaccag aatgactttc agcttcaatg gcatcgaagg
    11881 cacagagacg gggagagggc gcgactcatc aaacgcaacc cagtcaaaac gctcgcggtt
    11941 aacgatattt ttcagccgca tccacagcag aggttgcgcc agcagtgtac gcaaagagat
    12001 gatgagaata ccgccatttg cctgatgcac cagaccaggc tgcagggtaa tgtcgccatt
    12061 aaactggcgc aggcagccaa agagttgctc cgcttctacc cagtcggcag cgacaacttg
    12121 cgttaaagtc gcaaaattat catctgcact cactgcgtga cgtaagcgga tggagtggcc
    12181 ggaaacctca tagtgaccgc ccaccagttg gcctgcatcg ctttgtagcg tacgcgcggc
    12241 attggcaata agattcagat actcagactc ttccggggcc ttcgccagca taaaagagga
    12301 ggatgctcgc gtatgcagca actgctccag cgcaaattgc agccgcggtt gagtatcact
    12361 gaataaagga tcgttttcgt caatcaaatg tggctgagca aatatttcct gatagctatc
    12421 ggtatcagga accaggtcac gccatgcaag tttcgtaatg gtcaaagttg atgtttttta
    12481 gtctgttgtc aaagccgcga ttataccgta accggcacta cagcacacgt agaaagcacc
    12541 gacaatactc ctggcatggg cgttaaagct cacaggatgg agattctttt cttcactggc
    12601 ctaaaaagct gatattctgt aaagagttac acggtaacat tgagatcgct atgaaatatc
    12661 aacaacttga aaatcttgaa agcggttgga aatggaagta tctggtgaag aagcatcgcg
    12721 aaggggagtt aatcacccgt tacatagaag ccagtgccgc ccaggaagcc gtcgatgtgt
    12781 tgctctcgct ggaaaatgaa cccgtgctgg taaatggctg gattgacaag catatgaatc
    12841 cggaactggt caatcgcatg aagcaaacca ttcgggcaag acgaaagcgc cattttaatg
    12901 cggaacacca gcatacgcgc aaaaaatcga tcgatctgga atttattgtc tggcaacgtc
    12961 tggctggtct tgcgcagcgt cgcggtaaaa cgctttctga aacgattgtt cagctgattg
    13021 aagatgcgga aaacaaagag aaatacgcga ataaaatgtc ttctctgaag caggatctgc
    13081 aggcattgct gggtaaggaa taactgacga aagtcagttc aatttactaa aggcaaaaaa
    13141 aaccccgcag cagcggggtt tttctaccag acgagaactt aagcttgcgg ctgagttaca
    13201 acgtctttga tacctttaac ttcgatctct acgcgacgat ccggagccag gcagtcgatc
    13261 agtgcagcac gctgtttcac gttgtcacag gtgttgccag taaccgggtt ggattcgccc
    13321 ataccacgtg cggagatctt gtctgccggg atacctttgg agatcaggta atcaacaaca
    13381 gactgagcac ggcgctcgga cagaccctgg ttgtaagcgt cagaaccgat gcggtcggtg
    13441 taacccagaa caactacgga accgtctttc ggatccaggt tgctcagctg gctgtacagc
    13501 tgatccagag cagcctgacc ttccggtttc agggttgctt tgttgaagtt gaacagaacg
    13561 tcagacttca gagtgaagtg cttggtctgt acttccggtg ccggagctgg agccggagca
    13621 actactggag ctgcttcgcc ctgaccgaaa cggtaggaaa cacccaggct cagcatgccg
    13681 ttgtccggac gagtgccgat ggtgtgtgcg tcaccgatgt tgttggtcca ctggtattcc
    13741 agacgggtag cgatttcagg agtgatcgcg tactcaacac cgccagcgaa gaccggagaa
    13801 acgccggtgt cgtggttttt accataaacg ttggatttag tgtctgcacg ccataccata
    13861 ccacccagac gagtgtagat gtccaggtcg tcagtgattg ggtaacccag tttagcggtc
    13921 agttgaacgc cctgagcttt gtatgcaccg ttttcaacgc tgcctttgta cggcatacga
    13981 cctaaccagt cgtaacccat ttcaaagcca acatacgggt taacctggta accaccaaaa
    14041 gcaccagcgc ccagttggtt ttcatgggtc gggccattgt tgttgatgaa accagtgtca
    14101 tggtactggg accagcccag tttagcacca gtgtaccagg tgttatcttt cggagcggcc
    14161 tgcgctacgg tagcgaaacc agccagtgcc actgcaatcg cgatagctgt ctttttcatt
    14221 ttttgcgcct cgttatcatc caaaatacgc catgaatatc tccaacgaga taacacggtt
    14281 aaatccttca ccgggggatc tgctcaatat taactctacc gatatcttcg gcttatgccg
    14341 agcacccctg gcgatgtaaa gtctacaacg tagttgaaaa cttacaagtg tgaactccgt
    14401 caggcatatg aaaaaaaagt cttgtataag gtatgtttaa tcttttttgt cagcgacaat
    14461 ttacagaaga gaatcgcgga aaccgcttca gacaagcctc cgcaaggaaa attagtcacg
    14521 actgaaagca ttggctgggc gacaaaaaaa gttccaggat taatcctaaa tttacttaat
    14581 gatacaaatt agagtgaatt tttagcccgg aaagttgtct cgtggcgtga gaggatgcgc
    14641 ttaccggacg cataataaac cccatagcgt taccttcatt tgccgcatca acaagttcag
    14701 catgctcttc ttcagtcaaa tcatctgcca accaaccgat caccacactg taattgcccg
    14761 tgcgtaaagc gcgaaccatt gactccacag tgtggcaagg ggagagctgg ctaatctgca
    14821 ttactttcgt taagggtagc ccagatgcct gaacccattc ccgactcagt ttttgttgcg
    14881 gtgttaacca gagttgccag cgcgattgct gaccgagttg ctgtaacaat ggcaacagta
    14941 gaagttgcgt catcatgggc tgatcttcgc gatagacaac ttcactgata agcccggctg
    15001 tagtgttttc cgtagagaca cgcgcaattt tacttgctgc ggatgagaac gacgaagaac
    15061 gatgtgcata gcctgaagtg tacataatca atccagcccc tgtgagttac tgtatggatg
    15121 tacagtacat ccagtgacaa caaagatcaa ccctattttc ggaaagagcc tcgcaaattt
    15181 tgtcgttggt gacgggaaaa cataaattaa tcttgccgct taagaataag ttgcctattt
    15241 tcgtagttaa cggatccgtt aatgtgaatc attcttttat gttatgattt taaaaggaat
    15301 tttatgaaaa gcctctccta taagcggatc tataaatcac aagaatacct ggcaacgttg
    15361 ggcacaattg aataccgatc attgtttggc agttacagcc tgaccgttga cgacacggtg
    15421 tttgcgatgg tttctgatgg tgagttgtat cttcgggctt gtgagcaaag tgcacagtac
    15481 tgtgtaaaat atccgcctgt ctggctgaca tataaaaagt gtggccgatc cgttaccctc
    15541 aattactatc gggttgatga aagtctatgg cgaaatcaac tgaagctggt gcgtctgtcg
    15601 aaatattctc tcgatgcagc gctgaaagag aaaagcacgc gcaatacccg ggaaagactg
    15661 aaagatttgc ccaatatgtc ttttcatctg gaagcgattc tcggggaggt ggggattaag
    15721 gatgtacggg cgttacgtat acttggggca aaaatgtgtt ggttgcgact gcggcagcaa
    15781 aacagtctgg tgacagaaaa gattctgttt atgcttgaag gtgccattat cggcattcat
    15841 gaagctgcgc tcccggtggc acgccgccag gagcttgcag aatgggctga ctctcttacg
    15901 ccgaaacagg agtttcctgc ggaacttgag taatctggcg ttgcagacga ccaatctcag
    15961 gcagtaatgc aatcaataat ccaacttgtt gtacgaccag aggttctttg ctgtctgccc
    16021 gtggttcaag ttgctgcatc cgctgtttca ggctagctaa tgcctcattg acgcgttctt
    16081 catcagcagg ttgatgatgt aacgcgtcat caacatagca aactgcgtca tcaagaaacg
    16141 ccagaatttc aggattagtt aactgctccc ggtgagcacc gagggctgag atatagctgg
    16201 taaacgtatg gttaaggcac agcaaccgaa acgcggcttc gcgaatttgc ggggtaacgt
    16261 tcggctcgct ggacatattt gataccaccg acgccagctc agcatcacgg ttgtgtgcat
    16321 cgcggcgggc aatacgatac gccagacggt tatcacgccc ctgatggtat tgctccagta
    16381 tggcatcgag ataccgacag ttggcctctg tggcgcgttc gagcatgcgc ggcagattgc
    16441 gaaactgcca gtcaggccag atgtagctca ctgccgccca cgcaatggca caaccaatca
    16501 gcgtatcgat tacgcgaggt aacgctactt caaaaccttc acccagtaag ttaaaacaca
    16561 gtagcaccaa aagtgtgatg aacatcgttg catgagcgta ttgcacgtta cggaaggcaa
    16621 aaaagagcac gccggtaata accagcagca ccagctgccc ttccagtgat ggcacaaacc
    16681 acagcacagg aatgccaatg gcgataccta ccagcgtacc aataatcctt aacttcaggc
    16741 ggtggcgcgt ggcgttatag tttggctggc agacaaacaa acttgtcagc aagatccaat
    16801 acccgtgatg cattccggtt atctgaatga tggcgtagcc gaagcacaac accagcgaca
    16861 ttcttaccgc atgacggaag agggcggatt ccggcgtgaa gtgacggcta agacgcagcc
    16921 agatatcact caacccgtgc gggctgtcat cagcgagctc attttcgtca ttattatggg
    16981 gtagtgcctg ggcctgttct gattcaattg ttgccagttg ggcatcaatg gcgcgtaaat
    17041 tgttcagcaa aaatcccagt gttttgagta aatcggcggg tgcgccgtta tcgcgcatcc
    17101 gctccagcgc agcatcaata tgcgtaaaag cgcgctcaaa atgcggatca tgttgataag
    17161 gctgacgcaa caaaatacag cgtgacagtt gctggcacgc ctggccctgc atcgacatca
    17221 gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg ttcacgcaat gtttgatact
    17281 gaatatgaga agagctggca cgctcgtgaa tatcctgtgc gacaaagtaa taatgcagcg
    17341 tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg ggtcagcagc gagagtttcg
    17401 tctgattcaa tgtcgccatc agcagaccgt tggcgagagc caaatcgtac agcggtgcct
    17461 ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt gagctcaaga taacgcgcca
    17521 gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg gaccgggaac agcagatgac
    17581 caataagtgt caggacgttg taccagacgg caccggccag cagatacatc ggctgctgat
    17641 accagtgctc atacagtgat gttcccaaca tagtgtaaat ggcgatcagc aatgcaccga
    17701 aggcaattgt tgcatagcgt tgacccagac cgccgagcaa aatgaagccg ctggtagaga
    17761 gcgttaagcc aatcgcaaat agccagggcc agggaaacag caattctact gaggccgagg
    17821 cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa acgtcccgcc agtcggtcat
    17881 cgagatcggt cagcgccgct gccaccatcc ccagcgttag cggaatcgtc agttttacat
    17941 cacccagcca ccacggaaac gctgtggttc cacaaagcgc aataaaaata cgcgcgtaat
    18001 acagccaggc gctgttccag gtatagcgtt tgagcaaagg acttagcata aaggccatat
    18061 tagtggatta ttcaaaacga cgacgtgcat tggcttcacg cgcagcttgc gctgtttcta
    18121 ccgataccac gcgacgacca accggccata gtgcaatagc ggcaatttta aagttcgcaa
    18181 tgccgacagg aatgccaatg attgaaatac attgtgcgat gcccgttgca atgtgcatca
    18241 ggcataacca ccagccaaag aaaatcagcc agaaaatatt caataccgta ccgccagtat
    18301 tcagcagcac atttttgcca gccgggttca gttcatcgac atgaatagct tcattgccat
    18361 aaggcaccag agacagttta gtgatctccc agcaggatcg tgtcagcggt aaggtaaaaa
    18421 tcagcgcaat actgaccaga gtcgccaaca gccagcccag agtggtggca aatccgccaa
    18481 gcacaaagtt cagaatgttc aaaacggtac gcataaaacc tcgctttact gtggttttca
    18541 gtaatgggcg gcaattgtaa cgtttttttg ggctggagca cgttttctct gacggttaca
    18601 ctgataagaa ataatttcgt gtggatctac agagtcatgg aactgaaagc gacaacgctt
    18661 ggaaaacgtc tggcacagca cccttacgat cgggcggtga tcctcaatgc cgggattaaa
    18721 gtctccggcg atcgccacga ataccttatt cctttcaatc aattactggc gattcactgt
    18781 aagcgcggtc tggtatgggg cgagctggaa tttgtactgc cggacgaaaa agtggtgcgt
    18841 ctgcacggta ccgaatgggg cgagacgcag cgtttttacc atcatcttga tgctcactgg
    18901 cggcggtgga gtggcgagat gagcgaaatt gcgtctggtg ttttacgcca gcaactggat
    18961 ttgattgcca cgcgcactgg agaaaataaa tggctgacgc gtgagcaaac ctctggcgtt
    19021 cagcaacaaa tccgccaggc tttgtcggcg ttgccgttgc cggttaaccg actggaagaa
    19081 ttcgataact gccgtgaggc gtggcgtaaa tgtcaggcct ggttgaaaga tattgaaagc
    19141 gctcggttgc agcataacca ggcgtatacc gaagccatgc ttaccgagta tgcggatttt
    19201 ttccgccagg tcgagtcttc accgctgaat ccggcgcagg cccgggcagt cgttaatggc
    19261 gagcattctc tgttagtgct ggcaggtgca ggaagcggaa aaacgtcggt gctggtggcc
    19321 cgtgcaggct ggttgctggc gcgtggtgaa gcgtcccctg agcaaatttt attgctggcg
    19381 tttggtcgca aagccgctga agagatggac gagcgtattc gcgaacggct acataccgaa
    19441 gacattaccg cacgcacgtt tcatgcgctg gcgctgcata ttattcagca gggcagcaaa
    19501 aaagttccga tagtcagcaa actggaaaat gataccgctg cccgtcatga actctttatt
    19561 gctgagtggc gcaagcaatg cagcgaaaag aaagcgcagg cgaagggctg gcggcaatgg
    19621 ctgacggaag aaatgcagtg gtcagtgcca gaaggtaact tctgggatga tgaaaaatta
    19681 cagcgtcgtc ttgcctcacg cctcgatcgt tgggtaagtc tgatgcggat gcacggtggt
    19741 gcacaggcag aaatgattgc cagtgcaccc gaagagattc gcgatctgtt cagtaaacgt
    19801 atcaagttga tggccccgtt attaaaagcc tggaaaggtg cgctgaaggc agaaaacgct
    19861 gtcgattttt cgggccttat tcatcaggcg attgtgattc tggagaaagg tcgctttatc
    19921 agcccgtgga agcatattct ggttgatgaa tttcaggata tctcgccgca gcgggcagcg
    19981 ttgttagcgg cattacgcaa gcaaaacagt cagacgacgt tgttcgccgt tggtgatgac
    20041 tggcaggcga tttaccgatt cagcggtgcg caaatgtcgc tcaccaccgc tttccatgaa
    20101 aactttggtg aaggcgaacg ctgtgattta gacacgactt accgttttaa cagtcgtatc
    20161 ggtgaggtgg caaaccggtt tattcagcag aacccaggcc agctgaaaaa gccgctaaac
    20221 agcttaacca atggagacaa aaaagccgtc acgttattgg atgagagtca acttgacgct
    20281 ttgctggata agctctctgg ttatgccaaa ccggaagagc gcattctgat cctggcgcgt
    20341 taccatcaca tgaggcctgc cagcctggaa aaagcggcaa cacgctggcc gaagttgcaa
    20401 atcgacttta tgaccattca tgccagcaaa gggcaacagg cggattacgt cctcatcgtt
    20461 ggcttgcagg agggaagtga tggttttccg gctgcggcgc gggagtcgat tatggaagag
    20521 gcgctactgc caccggttga ggatttcccg gacgctgaag aacggcggtt aatgtacgtg
    20581 gcgctgaccc gggcacgtca tcgggtatgg gcactgttta acaaagagaa tccctctccc
    20641 tttgtggaaa tactgaaaaa tctggatgtg ccggtggcga gaaaaccgta agaaacaggt
    20701 ggcgtttgcc acctgtgcaa tattacttca gacggtccgc gagataacgc tgataatcgg
    20761 ggatcagaat atcgaccgcg tcgttgaaat gcggcgactg gataatgaag tctgccgttg
    20821 ccacgttggt ggcgaccgga atgttccata ccgtcgccag acgcagcaag gctttcacgt
    20881 caggatcgtg cggcacggca tttagtggat cccagaagaa aatcaataca tcaattttcc
    20941 cttctgagat caatgcgcca acctgctggt caccccccat tgggccactc aacatcgcgt
    21001 tgacgttcat gccggtcgcg cgggaaatta agttaccggt agtgcctgtt gcatacagta
    21061 cgtgttgttc cagtaacggt tgatgccgtt ccacccagct catcagcatt tgtttgcagt
    21121 gatcgtgtgc caccagcgca atatgtttcc gcgcaggtaa agtgcgagtc gtcagttcca
    21181 taatgtacat ccgtagttaa ctttcctaca gattactgta agcacttatc gctgcaagat
    21241 aaagaccgaa aaagcctgcg cacaggcaca aaaatctcag gaagatggtt gtttttccgc
    21301 ccattgcagg aaagtatttc gcgtttgtgg gtcagccagt ttaaaccaat acttcagccg
    21361 ttgttctgtg agcacctgag actgcggtgg aatagcatcc agctcggaaa cacccgaaag
    21421 cagcgtactg tcatctgcca tcatcgtggc aaattgcggt agtgaagcgc gtttcgcgga
    21481 tttgttatag cgttcaacct cgacctcata atcaatcgtt tttgccgttg aggtaatggc
    21541 gagaatatcc agctttaccg gaatcggcgt cgcatcgcca tccaacaatt caaggcgcgg
    21601 cgcggcatca aaatggttag cttcccgctc attctccagg cgaggcaggc gaaaattgac
    21661 ctggttgatg agctgggtat taaaactgac caccagcggt ggggagatat acagccgttc
    21721 ttcactattg gaaagatgaa tcgtcttctc aacgcgaaac actaactgat gcggtccatt
    21781 atccagttca atgctgtccg caccgcgcag cagagagctg gaaaccttct tgccgtcgag
    21841 taccagaaga tcgacatcgg ttgaaagccg cagcgtggtg gcaaaaacgg ataccggcaa
    21901 acataatgca atcaaggtgg tcacgatgcc ggttttcata ggtagctcct gtcaaaagac
    21961 cgcccgaata ataatgtgtc aaaatttttc tttaatcata cttactaaca tatagacata
    22021 tttcccgctt ttgctctcat tcattcgtat tagctgcatg gttggcatgt cggctttgtc
    22081 gtacactttg tgaaacagtc tggaggaaat aatgaaagaa accgatattg caggcatttt
    22141 aacgtccact cacaccattg cgctggtggg ggcaagcgac aaacccgatc gtcccagcta
    22201 tcgcgtgatg aaatatctac tcgaccaggg gtatcacgtc attccggttt cgccaaaagt
    22261 tgccggcaaa acgctgctgg gccagcaggg gtacggcacg ctggcagacg ttccagaaaa
    22321 agtcgatatg gtcgatgttt tccgtaattc agaagcggcg tggggcgtag cgcaggaagc
    22381 tatcgccatt ggcgcgaaaa ccctctggat gcaactgggg gtgattaatg agcaagcggc
    22441 agtactggca cgggatgccg ggttgaatgt ggtgatggat cgctgcccgg ctattgagat
    22501 ccctcgcctg ggcctggcca aataaaaaat ccccggaagg caaaaacctt ccggggatct
    22561 gttcagggat tagttacgca gacgcggggc ctggagttgt ttgcggatgg tctgcgccag
    22621 ctcatccatg gatggctgtt caggatgttc atcctgcaat tcgctactta gctgggcttc
    22681 agccaggtag gtatgaaccg gtaggccgtt atcgtcctcc atcaccacgt gataccacgg
    22741 agcggcgcga agctcgtcat tcaccgccag ctcatcaggc gacggttcag agagcgaata
    22801 aaccgggtcg atatccacga ccactccgag ataacctaac agggaatggc ggacctgctg
    22861 gccgataccg aatttgctgg caatcatagt cacctcccgg gaaatcttta cctttgatat
    22921 aagggtaaaa ttccacattt caagttacat gacgcgacag gcaaaccctt tcagatatag
    22981 cccttccgga taggtagcga tcaccggatg atcggccgcc tgacggaact gctctataaa
    23041 ttgtacatca cgaccggcat caattgcggc atccgcgatg attttctgaa ataaatcgct
    23101 ggtcatcaga ccggaacagg agaaagtcag gagaataccg ccttcattca gcagctgaat
    23161 cgccagcatg ttgatatctt tatagccacg acacgcgccc atcaactggc ttttattctc
    23221 aacaaacttc ggcgggtcca tcacgataac gtcaaatttt tcaccgcgat cgcgataggt
    23281 acgcagcaat ttaaagacat catcacggac aaactcagcc ttgctcagat ccagtttgtt
    23341 cagctcaacg ttctgccgtg caatatccag tgcttcctgg gaggtatcaa cgctgacaac
    23401 ctggctgcaa ccgcccatca gtgccgatac ggcgaaacca ccggtatagg agaaacagtt
    23461 cagtacgcgt ttattttcaa cgtagcggcg ggtagccagg cggctgtctc gctggtccag
    23521 gtagtagccc gttttgtgtc cgtgctgtat gtccaccagc agcttcatgc catgttcttc
    23581 aatcggcagc agggcaggcg gcaactcacc ggtgacgagg ccctgggtca gctccatccc
    23641 ttctttttta cgtaccgcaa catcgctgcg atcgtaaatc gcacattccg ggtacagcgt
    23701 ttgcagggca ctaattaatg ccgcgcgctg atattctgcg ccagcactca gcagttgcag
    23761 caccagaaaa ttaccgaaac gatcgatagt aatacccggc aggccatcag attctccggc
    23821 gattaaacga tagctgtcga ggccatcttt ttgcgccagc cagtcacgcc atttttgtgc
    23881 ttgttgcaaa cggcgggaaa aaaaagcaat gtcgatagac tcagacgggt caaacgtcca
    23941 gacgcgcgcc cggatttgcg aagctggcga ataagcgccg cgtgctaacc attttccctg
    24001 atgatcaaca atatcgatgg tttcaccgag gctggcttta ccttccatac gggcaacggc
    24061 cccggaaaag acccacggat ggcgacgaag taatgatttt tcgcgccctt tggctaacac
    24121 taaacgtaca ctcataattt acttttctgt cgatgcaaag aaatgggcgt cattgtccgg
    24181 agttttccat ggatttgcaa cgcgtccatt aaggataagg agagcagcaa tgtcgaaagt
    24241 ctgcataatt gcctgggttt acgggcgagt tcagggcgta ggatttcgct acaccacaca
    24301 gtacgaagcg aaaagactgg gcttaaccgg gtacgccaaa aatcttgacg atggcagcgt
    24361 ggaagtggtt gcctgcggtg aagaagggca ggtagaaaaa ttaatgcagt ggttaaagag
    24421 tggcggcccg cgttctgcgc gtgtagaacg ggtgctcagc gagccgcatc atccctcggg
    24481 ggaattaacc gattttcgta tccgttaaat acattttacc ggttttggca ggccagcaat
    24541 tttggtggct tgcttagccg gacctttcgg aaacagtcgg tataaatagc ggctgttacc
    24601 tttttcttcg ccaaatttat tcgccatcgc ttttaccagc atacgaatcg ccggagaagt
    24661 attgaattcc agatagaaat cacgcacaaa acgcaccact tcccagtgtt ctggcgacag
    24721 cgaaatccct tcgttctctg caatcaccac tgccagcggc tcactccact ggctgctttc
    24781 tttgagatag ccttcggtat ccgtttctat ctctttacct tcgaagatca gcataattat
    24841 tactacctta atcagactgc cggcagtgta acaaaaaaca aagccccgca taaagcgagg
    24901 ctatgaaagt gttagcgggt gagattaatc gcggctggcg aagcccagaa tgctcagcag
    24961 gctgacgaag atgttgtaca gcgaaacata caggctaacc gtggcacgaa tatagttcgt
    25021 ttcaccgcca tgaatgatgt tgctggtttc aaacaaaata gcgccagagg agatcaaaat
    25081 gaagaccgcg ctgatcgcca gatgcagagc aggcagctgc aggaagatat tagcaaccat
    25141 accaatcagc accaccacaa tacccgccat cagcataccg ccgaggaacg acatatcttt
    25201 gcgggtggtc agcacatatg cagagcagca gaagaacact aacgccgttc cgcccagtgc
    25261 catagcgatt acgtcaccca ttccggcaga cagataggta ttcagaattg gtccgaggat
    25321 ataacccaga aaaccggtaa aggcgaatgc ggagataatc ccggtcggct tattcgccat
    25381 tttataggtc aggaacatca aaccatacat acccaccagc gtcagaatca gacccggaga
    25441 tggcagcatc agcaccgtac tggcagttgc ggtaatcgcc gaaaaggcca gcgtcaggct
    25501 cagcagaaaa taggtattac gcagcacttt atgggtgcta agcagtgatg tacggtcatg
    25561 tgaagaacta acaatacgat ccattgagtc actctctatg acagatgtaa ttaattaagc
    25621 agcataatga taatgcgtaa gggcacccag tagttttacc catctttacg catttgatct
    25681 ggaacaggtt taacagcgga ttatcaggtc attaagcaaa tataacgccc tgagaatttc
    25741 ggcaggcaaa agaaaaaggg gttagcattt agctaacccc ttatcttatc tggcggaagc
    25801 gcagagattc gaactctgga accctttcgg gtcgccggtt ttcaagaccg aagaaaaaca
    25861 aataaaaatc agtgtaatac gtgaaattgc tgaaaagtta atcaaattta acaccaagaa
    25921 aaatcaaggt attaggaagt tgctcatagc tagtatacca agtctttttg ccagaaattt
    25981 ttctggattg tctacttgct aaccacgagc ggcaaatcca gtgttggtgt gatttttgtt
    26041 ttacgatcgt aaatcaacac ctgattttct gttttatgcc cgctgaaaat ttgtttgtcg
    26101 cgactgctgc cttcgtaatc tgagatcccc ttggctttta tgtcgtgaaa attgcacccg
    26161 aacggaacgc caactttttg ctcggctgcg cgtttagcct gattccacca gttattcagc
    26221 gttttagcta tgactttccc gcctttggtt gtattgatca catattcgca tgtacaggaa
    26281 gatacatttc gggctaactg gatcgctgta cgtaatcgcg gtgaccattc cttgatttgt
    26341 ttggtgccgg ttttgttttg ctcaatgtag atccctttat ccataatatc ctgccatttc
    26401 aactcaagca catcaccgag tcttgccgca cagagatagg aaatctccat tgcaatgcgt
    26461 aactgtggaa ttgcttccgc atatatcgcc gcatactgtt catcggtgat gtaaacagtg
    26521 cgggctttaa gagagaattt tctgactcct ttgcatgggt tattcttcac atacccacgc
    26581 tcatatcccc acccgtatac tcgactcagg cttgccagtt catgatttgc ctgggtcttg
    26641 ctctcaagcc ctcgtttatc catgaaaatt cttacctgct cagttttgac attatcagca
    26701 agcacttttc cgaataccat cagcaacgcc ttctgatgtt gtcgataatc tttttgggtt
    26761 cggggggcca gttctgtaaa tgcaggggag tccataaaca tgtgccacaa tttagctacg
    26821 gtcattatgt tgtggagttt tgctttttcc agttcataat tttgccagac tttagctacg
    26881 ctggttttcc gcactcttcc tagccctata cttcttgtac ttccttcggg tttccacacg
    26941 taactgtaac cattcgatct aacccgcggt ggcagtacat tatctttttt attttttctt
    27001 ggtcttccca ttgttcagcg cctcaaaatc gggttcagca gaaaccagtt cagatgtttt
    27061 tggcatcgtt gtcagaccgt gtggaatatc cctgcggaga actatgggtt cgtttttagg
    27121 gccgattaca aatgggatgc cgtgcagtct taactggtgt tgctgttttg tgtatcgctc
    27181 gtatttcgtg atctctttaa tctctgctgg agatagagtt aattcgtaca tgtggtcacg
    27241 tgcctttaca gcatgaccgc cgccactata gctggtgggc ggcgatatgg gttgaacatc
    27301 aaaaatcagc ctgactcggg agcagttttt gccagatggc tgaaacgtat ttcgcctggt
    27361 aacg
//
LOCUS       DAZFBR010000067        26853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFBR010000067 DAZFBR010000000
VERSION     DAZFBR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.067"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..167)
                     /locus_tag="ACHJZ2_002964"
     CDS             complement(<1..167)
                     /locus_tag="ACHJZ2_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569038.1"
                     /translation="MLAQLFEQLFQSIDSTLITNIFIWAVIFVFLSAWWCDKKNIHSK
                     FREYAPTLMGA"
     gene            379..3738
                     /locus_tag="ACHJZ2_002965"
     CDS             379..3738
                     /locus_tag="ACHJZ2_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HFO4569039.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQSSQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPHSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWYSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQGII
                     MLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSLMLENTVDLRVSWKDGSNLKL
                     LLPKPVSGGRFVTNDGSVHFDGVASIAHLHGIDAELLTISCAGRGYLNIELLDENPVA
                     EKFRYLHADLPLLSGRNDKLQQISLYENYNLLNAMLACAWNSNSTLCVDFYSDRFGKD
                     KATLNIKRYDGSFIEHDQGLLVDIKNSVVFPANRIDELVVDAISLKNPGLHISLLKKD
                     EFAYDLSALNVQDSPWLIVGKLDGTARIAPVIKWMLPVLQTNDLLLNALCEADPEQRK
                     KNFNELIFEIDNNPLQNYCCLLTEYIKKYKMNNGLSLLDLDLFRGISSNYRVVVQLLI
                     SSCLSGDSDTIYDIQEELPFSWGWIPVSIWKDVFQKCWTYLEKQINDKTLALHILQPF
                     IAFMNHRAHIDRRLAPIANMLLTYSESLPTGCDVLPTVSREQFNEAKQMLLRNPDSFG
                     RISIFPKELWSSAITPELKSVFNKLWIEDKYHSRLEKRFNLMLVAALLTQKDNNLIHQ
                     LSALFEFHYQQAPQQLGVIYQYYFEQAGVCH"
     gene            3738..10052
                     /locus_tag="ACHJZ2_002966"
     CDS             3738..10052
                     /locus_tag="ACHJZ2_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4569040.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            10276..13134
                     /locus_tag="ACHJZ2_002967"
     CDS             10276..13134
                     /locus_tag="ACHJZ2_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4569041.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHMENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            13137..18071
                     /locus_tag="ACHJZ2_002968"
     CDS             13137..18071
                     /locus_tag="ACHJZ2_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569042.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFICKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSL
                     NSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQA
                     LVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGL
                     PRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEG
                     ERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            18071..24412
                     /locus_tag="ACHJZ2_002969"
     CDS             18071..24412
                     /locus_tag="ACHJZ2_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4569043.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            24409..26523
                     /locus_tag="ACHJZ2_002970"
     CDS             24409..26523
                     /locus_tag="ACHJZ2_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO4569044.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
BASE COUNT         6960 a         6188 c         7003 g         6702 t
ORIGIN      
        1 aatgccccca ttaaggttgg agcatattct ctaaacttac tatgtatatt ttttttgtca
       61 caccaccacg ctgataaaaa tacgaatata acagcccaga tgaaaatatt ggtgatcagt
      121 gtagagtcta tcgattgaaa caactgctca aaaagctgcg ctaacatttt gatacctgtg
      181 taaataatgg atagttgtaa tcgtttaaaa aataaacaaa acacacataa tttatatggt
      241 taagttcggt gtagcgctca tgattgtaac aagttttttc gagatataaa tgatttgtct
      301 catttttagc gagttcatcg ggttgagctt ttttatgatg atagtaacct gttacgaatt
      361 gtatcaaagg aaggcaggtt gcttgtgaaa actccagtta acccactttt gcaatggctt
      421 aacatgtttt ttagtcgccg ttcattatcc ggagctgatg gacgggcgtt atatgcttac
      481 cgttgtactg atacagagta cgaaagtttg gcagaactac tgcgtacata tgccccccgt
      541 agttatccaa gaacgatatt catttcctat agcgatgttc tatttagcat atatgccgca
      601 gagtttatcc gccggactca tacggtcgga caccctaaat gggacacaat tttagattct
      661 attaactgga aagttccgta tgttcatcga cagaagctgg ttaatgatgg cattcgctac
      721 tggaaaagaa agataagaaa cctgggacaa gcttcgggtt atctgcatac actggcttgt
      781 gaaggtggtt tgcctatccg catgattgaa aatgagagcg gttatttgat tacctatttc
      841 agaaggatat atcaggcgct tcgtgggcaa tcatctcaat atcctgccgc taaaattgca
      901 caagaattag gcgatacgat tcctgtcaca atgcaaaacg aactggtcta tgaaatagcc
      961 ggtgaattct gcgagactct ctgccgatta cttagtgagc atcctcctca tagcagtgat
     1021 cccgtttctg cattacgtaa gctctctcca gactggcatc ttcaacttcc actcgttctc
     1081 cccgaagcga atgctgcaga gattgtaagg cgattacttt ctcaatcttc tgaaatacgc
     1141 agtgcaagta gtcttcaggt tgaaagaatc tgggtcgatg ttgatgacag ttggtattgc
     1201 gatgcccggt tccgattccc ggccacaatg cgtacagaac agttaacctc tttgtttgaa
     1261 tgccatattc agccggagca gacccgactc atcatttcag gaaaatggaa aaatggcggg
     1321 gcaaggttgg caatgctaag ccgttatgag cagcaagatt ggcgggtcga gttattacct
     1381 attgcgatgc aaaaactctc tggtgcagat gcaatggccg aaatctcatt gtcgcttcat
     1441 gaaggtccaa ttctgttagg tcacacaatt ccaaaaggcg gttatgaact tactgaagaa
     1501 cttccctggg tttttgaagc gatgaatgag agtgaatcgc agctaaaact tgttggtatg
     1561 gggtctgtga gttccaggct aaatgctctg tttatttcgc taccgaaaaa tagccattta
     1621 gatattagtg gagaaggtga gtttgatatc ccaaggttgc tgaaaaacag tgaacgcagc
     1681 ttaactaaaa taagtggagt atttagcgtt gttttacatg atggtgctgt ttgtacaatt
     1741 cgcacccagc aactttatga ttctgcgatt gaatattata ttaaatcgac agaagttgaa
     1801 ctggttaaat cggattatcc tgtccatcga gcctggccga agatcggttg gaaaaaggat
     1861 ctgcaatatg gcattgtacc ggagaaagaa cttttttggc gttccattcg ttcaggtaat
     1921 aatgcctggt attctgtcgc atcagaaatg ccaaaaggac agatagaagt ccggcgaata
     1981 gttaatgatg aggttttatt tagcggtaaa gttgtagttt taccagcaga cttcgatatt
     2041 aatattatac ctgagagtgc tcagcaaggc attattatgc tttctggtat aacggatact
     2101 agaattgata aatattcaaa caatgaaaaa gtaacactta agtccgatta ttcacaaaac
     2161 gagtgtgcta tttattataa ttcatcgctg atgctggaaa ataccgttga tttacgggtc
     2221 tcctggaaag atggttctaa tcttaaacta ttattaccta agccggttag cggtggccga
     2281 tttgtaacta atgatggttc tgttcatttt gatggtgtgg catctatagc acatttgcat
     2341 ggaatagatg ctgagttatt aaccatatca tgtgctggaa gaggatatct taatatcgag
     2401 ttattggatg aaaatccagt agctgaaaaa tttcgctatt tacatgccga ccttcctctt
     2461 ttatcaggac gcaatgacaa attacaacaa atttcacttt atgagaacta taatttgcta
     2521 aatgctatgc tggcatgtgc atggaacagc aatagtacat tatgtgttga tttttactct
     2581 gacagatttg gaaaggataa agcaacactc aatattaaac gctacgatgg tagttttatt
     2641 gaacacgatc aagggttact ggttgatata aaaaattctg ttgtttttcc tgcaaataga
     2701 atagacgaac tggttgttga tgctatttct cttaagaatc ctggcttaca catatcattg
     2761 ttaaaaaaag atgagtttgc ttatgatctt tcagctctga atgttcagga tagtccatgg
     2821 ttaattgtgg gaaaacttga tggtacagct cgcattgcac cagtaattaa atggatgcta
     2881 cctgtattgc agacaaatga tttattacta aatgctctat gcgaagcaga cccagaacag
     2941 cgcaaaaaaa attttaatga gctaattttt gaaatagata acaacccatt gcaaaattat
     3001 tgctgtttat taacagagta tattaagaaa tacaaaatga ataatggctt atccttgctg
     3061 gatctggact tgttcagagg tatttcgagt aattaccgcg tggttgttca attgttaata
     3121 tcatcatgtc tttctggtga tagcgatacg atttatgata tacaggaaga attacccttt
     3181 tcatggggat ggattcccgt ttcaatctgg aaagatgttt tccaaaaatg ttggacttat
     3241 ctggagaaac agattaacga taaaacatta gcattacata tattgcaacc ctttattgct
     3301 tttatgaacc atcgtgcaca tatcgatcgt cggctggctc cgattgcgaa tatgttactt
     3361 acatatagtg agagcctacc aaccggttgt gatgtattgc caactgttag tcgtgagcag
     3421 tttaatgaag ctaaacagat gctattaagg aaccccgaca gctttgggcg tatcagtatc
     3481 ttccctaaag aactttggtc tagtgctatt actccagagt taaaatctgt ttttaataag
     3541 ctttggattg aagataaata tcactcacgg cttgaaaaac gttttaattt gatgttagtc
     3601 gcagcgctgt taacccaaaa agataataac ctgatacatc aactgtctgc gctttttgaa
     3661 tttcactatc agcaagcccc acagcaatta ggggtaatct atcaatatta ttttgaacaa
     3721 gcaggagtat gtcattgatg gacgttcagc aactgcatta tgcgccactt ctaaatcagc
     3781 ttcatcagcg tgcggtgagg tcggtggtaa gtcagttggc attgcggagc aagtcgttga
     3841 ctcattatct ggaatcacaa tattttcagc ctcccggtca ggaggggtca ctgcttgcag
     3901 acccggtatt cgaaagtaca ttcggctgga tgcctgcgga tgaaacaatg gaggatttgc
     3961 gggacaagct gatctccagc ccgctggttg atgcattgag caaagcgcca gtgccatttc
     4021 gtcatcaact tactgcgtgg cgaacgattc tggaagacaa aaaatccttg ttagtgagca
     4081 gcggtacggg atccgggaaa acagaatgct ttatggtacc ggttctggaa gatcttattc
     4141 gtcagcaaaa atcgacccgc cagtcactaa cagggacaca agctattttc ttgtatccgt
     4201 taaatgcatt aattaacagc caacaggagc ggcttcgcga atggacgcgc ggatttaagg
     4261 ggaaaatacg cttcgcgcta tataatggcg aaacccggca taccaaatac gaaattcagg
     4321 aagatcagtt gaaagtacct gaacaagctt tgagtcggga agccatttat gaagcaccac
     4381 cttccatcat ggtgacgaat accaccatgc tggaatatat gctgattcgt cgtaaagatg
     4441 ccccaatcat cgaaaagtct caggggatgt taaagtatat cgtcctggat gaagcccata
     4501 gttatattgg ttctcaggct gctgagttag cgctgctttt acgtcgtgtt atgcaggctt
     4561 tcaatgttgg acccggcaca gataaacctg tgcagattat cgcgacctct gccactattg
     4621 gtgaagactc tcctgaaggt aataaggagc ttgctaaatt tgtcgctgat ctcgcgggcg
     4681 ttacagaacg tgatgtcaaa gtggttcgcg gttatcgaca aataccgcgg gtatcggagt
     4741 cgctgattcg tcatgaatac cccacatctt taacttctct taaatccctg agccctcagg
     4801 atctttacca acagctttgt cattaccgtg tggcccagca attgcgtcag gcgttaactc
     4861 atcccggacg gcaggcagta cgtttgagtg agttgctcaa tgttgccaga agaacctggc
     4921 cagatattaa tcaccgtgag ctactgcaac tcctcgatct aatggcaaga tcgcgagaag
     4981 gtgaactggc ttttacgcca ttacgtatgc atgggtttat cagaacactg gcgggtctat
     5041 gggcatgttc aaacaaacaa tgctcacata aagcacatga attaaatcag agtgactggc
     5101 catttggtca ggtatggttt gagcagcgcc aatattgtga ctgtggagcg ccggtatttg
     5161 aagttttacg ttgctccggg tgtggttcag cttatttgtc tgccaaagag gagatgcgcg
     5221 gtgatggaac gaactggttg gtagcgcaac cagccgctgc cgaagtggat gagtttgctc
     5281 ttgatgtaga tgtttattct gaagatgaag acaacgacga attagacaat aactctcaat
     5341 ttgatcgtct gattgcgagc gatggtgaaa aatatatcat ttcactggaa gaaacgacgg
     5401 cggggaaaat tgatagtgaa gcgcaaaagc actatgaaat aaacctgtta agaccggaat
     5461 ctcgaggtga gggacgcaat aactcctttg cttgtgtttg ctgtggtgac actcaacgga
     5521 aaaataatcc tttattccgt ccgcttcggc tcggcgcgcc ctttttttta aatgaaatta
     5581 ttcctactct gctggagttt tctccattac cgcaaacaag agaagagcag ttaggaccgt
     5641 ttaatggtcg acgcttgctc acctttacgg attcgcgtca gggtaccgct cgcatttcag
     5701 cccgcttaca gcaggatgcc gataaagcga cgttgcgagc tctttgttac caggagctgg
     5761 caaatattac agagccttca gcaaaactca gcccggcaca ctgtgcactg ttagagaaag
     5821 ttattagcca gttttctgtc ctccctttaa aaaattccat cgacgcgctg aaaaaagcga
     5881 tggctgcgaa tgaggagctt agcccgacgg acattcaggc acttaaaatt attgagccga
     5941 tggtgagttc cttaagcacg gagcatgctg aagcgatgca aatacttcag gctaactctg
     6001 gcaatcagca gaagaaaacc atgtcatggc aaacgctgtc tgaacagctg gttggcagtg
     6061 tggaccttgc cgatagaatg agaggcagtt ttaaagaatt gtctgggctt gatcttacta
     6121 aacaacagtt tgcagatttt tgtttataca gcgagtttgg tcgccgccct aaaaatgcct
     6181 ggaccctcga aagtctgggt ctggtagcca ttcactatcc attcattgat aaagttacaa
     6241 cctgcccgca ggactggaag acattattac ccgatcccga taagcaatta gcggaatggc
     6301 gtaatttatt gaagattact cttgatttct ttatccgtga aaacagtgcc gttttttacg
     6361 aaaacgagca ataccctcgc tggatggggg cacgtttccc ggtaaaaatg ctccaggggc
     6421 cagataaaaa aaacaaaggg cagaaacgtg atcaactttg gccacaaata cgcgatcgcc
     6481 attataaccg ggtcattaag ctattaatcg cagtctttcc ggcaatacaa cctgagcagg
     6541 ctcactggaa atcgttagtt aaccacttaa tgattgaggt ctgggatgca attcgaccct
     6601 gcttacgtca gtttgagtcg ggttatcagc tggatattaa acaacaagct gaattttaca
     6661 gtccaaagca agtgtggcgt tgcccctata ctcgacgtgc gttagacgtc acgttgctgg
     6721 gatactctcc gtatttgccg ggtagcaaag aaattgcacc tgaaaaagca gtgttgattg
     6781 aaatgcctga gcttcctgta cgtcactggc gtttgtctgg tggtggagaa atcgcccgag
     6841 aagaacgtct ggagtggctg gaaagtaatg cgctgattca acatgcccgc gaggaaggat
     6901 tatggtctac tcgcagcgac cgactggccc tgaaagatag ctggtatcgt ctggaggaac
     6961 actctgcgca gagaacgcct gagcagaacc agtttaatga aaaacaattt aagtcaggca
     7021 aagttaatgt gctgaactgt tccacgacca tggagatggg ggtggatatt ggtggtatga
     7081 gtttggtggc aatgaacaac gttcctccag cgccggcaaa ttatttgcaa cgtgcaggtc
     7141 gcgcggggcg tcgtggagag agtgcatctg ctgccattac gttatgtaaa aacacggcgc
     7201 atggaatgga ggtattcaaa gaccccctct gggcgttcaa cactactgcc agtgcgccac
     7261 gggttcgttt tgggagcagt agtattgttc aacgacatgt taatgccctg gttttaggtc
     7321 tgtttttacg agctgaagtt cctgatgcga caaaactgag ttgcaaatgg ttctttgaag
     7381 gtgatgagag ccagtgtctt cgttttctcc attggttaaa tcaccaggcc gatcagctgg
     7441 cggataaact taaacgcctt actcagggaa ccgtactgat gtcattgacg gcaacccagc
     7501 tcttgacccg cacacaagca atgatgcagc aggttgatat ccgctggaga agccaacttg
     7561 cgatactgtt ggaaaatatc gaagcgttga aggcggataa cagcgcgtgg gaagaaacac
     7621 cagcaggtaa agccatcgct tatcagcttc gtgattatcg cggtgcatat ctgttttcta
     7681 aactcattag tgaagcgttc ctgccggggc atggtttccc ggttggcgta gtcaatttca
     7741 attatctgac ggcagatgag ctggaaaaac gtcgcgcaat aaaagccact caggctgacc
     7801 ctaatgaagg gggagaaagc ttttctcgtc gtattgaaaa attgcccagc cgtgatttgc
     7861 ctacagcatt acgcgagtat gcgcctggtg cagatgtggt attgggcgga aaagtatatc
     7921 gttcctcggg cattatgctt ggcaaggtgc ttgccagcgg gcaggagctg tcaggcgatc
     7981 atcatattcc gtggttctgg cattgccgta aatgtggtgc gggggcaacc agtactaccc
     8041 atcctgtcga atgtagtcac tgcaaagcgg atatacagca acttgatgtg aagcgttatt
     8101 tacagcctgt tggttttgcg acagatattc gttatcaggc acataatgat gtatccatgc
     8161 ctgcacaact tccctggaaa gatccacgtg ttcttgtacc gtcttcggtg tgggtttctt
     8221 tacccgatgc tgggctagga cgttaccgtt ttagccatag tggggaattg ttccacttca
     8281 gtgaagggga atttgggcac ggctacgcaa tttgcctctc atgtggtcgg gcagaatctc
     8341 aaactcaacc ccagcgtacc cctgaaaatc taaagaaccc agagagggaa aatacgcatt
     8401 acctgttgcg agggggcagt aacgatcgtc aggggagcaa taaattgtgc cacggtcatg
     8461 tgcataaaga tctgtggttg ggatatagca gtcgtacaga tatggttgag ctgcaactca
     8521 atgatgataa tggcctgttg atccgtgatg aggttgccgc gcgttccctt gcggtcgctt
     8581 tgcgtgaagg gttagcgcat aaattgggta tcgaaaatac tgagctgggc gtgacgacgc
     8641 agcaagcgcg cgatatcaat ggctataccg gatattccat cttcatttac gacaataatg
     8701 cgggtggtgc aggttatgca gtgcaattga tcgaccattg ggctgatgtc tttaattacg
     8761 ccagaaaatt gctcgattgc agttgcgata aattctgtca ccactgcttg ctcagctacg
     8821 acagtcagca ttatgtcaac agattagatc gtcaccatgc attaaccctg cttaccaatg
     8881 tacggttgca gcgacttaac ctcgcgccag aatatcagta cttcggcgat ggtagccggg
     8941 ttgaaactaa ccctctgagt ttacgtattg cgcaatgcct gaacagcgag atttatgact
     9001 cctgctcgct ggttcttgcg gggccgcagg aacagtggga ctttgcccaa tggccattat
     9061 ttaaagagtt gctccagttt gcctcctccg gtgggaatgt tgagttgctg gtggccacgc
     9121 cgctggcaaa cttaactgat agctccagac atcagctttc cgcgttggcg gcgatgccgg
     9181 gaggtcgctt acaagtgaaa tccattgcca ctgcgcagct catgcaagga aaggggcgct
     9241 ggcttgcgca ggtgactcgc gaagggcaaa gccaacagtg ggccgcagac gacagcgcta
     9301 ccgttgcacc tggtgaatta tggggacaaa gtgcgtcttc accagtagtg acacttaaag
     9361 gaacgtccgg aaaaaccttc tcgggtcaga ccttaagtgc agaagattta ctgcccgcct
     9421 tacccaccgg ggcggtgcgc atcaatctct gtgaacaact ggacgggccg ctggaaggtt
     9481 ttggttcgcg tttctggtcg ttagtcacgc aacagcacgc tggctggaag caggcattta
     9541 cccgccacaa agagataacg catgttgagt acagcgatcg ttatcttaat agcccattca
     9601 cggcgcgttt gttaggtgaa atcttaaccg agctggttga acagggaatg gcggaacgcg
     9661 ccagcctcac tgtttgcgtc aaaaaattgg attacaactc tcgccaacac gacgctctct
     9721 ataacgcgtg gcttaatgag gaagatcgtc agcaggttgt gaccacttta cttgaagaag
     9781 gatatctggg gcctgcgtgg ccgggagcaa ttagctggtt aacgggggat aaccagagta
     9841 cggagcatgg acgagaactg acagtcacct tcagcgatgg cagtcagcat tatgtcctgc
     9901 tcgacatggg gcttagctac tggcgctgta ttgaagatac cttctttgat tttgccctgc
     9961 gagtaccgca gcaagttgaa cggctggcaa acacgcgagc gcgagctgtt gccccgggta
    10021 atgaccttag gagttatatt atcgctggat aaataattgc tcccctgggg atatcttcca
    10081 ggggagttga cccagctcac tttttaataa tttttgtttg aaaatcatgc gcaatttatg
    10141 cgcattccct cccattttgt cactttgccg ggggccttca tgatatcttt aaggccctct
    10201 tttttatcgc agtgtgccgc gccacatttt tcaggatgac gttaccgcca ctgccccaaa
    10261 tagacagaaa aaccgatgaa taagcaaaat tatgcacctg gtatgcgggt ggttattcgt
    10321 gatgccgaat ggcgtattcg ccgggcggat gacagtggtg atggtgggta tttgctgacc
    10381 tgcgatggta tttcagagct tgtgcgcggt aaagaagggt tatttctgac caaactggaa
    10441 caaaaagtag agatcctgga tcccgcaaaa acgcatttgg tggaagatga atccgccaat
    10501 tatcaagcgg cacagttgta tattgaaagt cagttacgcc aacgcgtacc gacagacagt
    10561 aaagtccatt ttggtcatct ggcggcgatg gattccatgc cgtttcagct cgatcctacg
    10621 cggatggcgc tggcacaacc gcgtcagcgg atactcattg ccgacgctgt tggtctgggt
    10681 aaaacgctgg aagccggtat tctggtttcg gaactgatcc gtcgtgggcg cggaaaacgt
    10741 attctggttc tggcggttaa atcgatgctg acacaattcc agaaggagtt ctggagccgt
    10801 tttgcgatcc cattgacccg cctggattct gctggtttac aaaaggtgcg taaccgcatc
    10861 ccaaccaacc acaacccgtt ccactatttt gacaagacca ttatctccat cgatacgctg
    10921 aaacaagaca ttgaatatcg tcaccatatg gaaaatgcct ggtgggatat tattgtcatt
    10981 gatgaggccc ataacgtcgc agaacgtgga accagttcgt tacgcagtaa actggcaaaa
    11041 ctgctcgccg ggcgcagcga tacgctgatt atgctttcag caacaccgca tgatggtaag
    11101 gctgaaagtt ttgccagcct gatgaacatg cttgacccga cggcgatcgc caacccgaaa
    11161 gagtatgaat acgccgattt tgccgataaa aatctggttg tccgtcgctt caaaaaagac
    11221 gtgaaagatc agatgtccgg cgagttcccc gagcgcaata ttgtcaaatt gactcgcctg
    11281 gcatccggtg ccgaagaaga agcataccgt cgtctggttg agagccagtt ccgtgatgat
    11341 gacgatgaac aggcccagtc taacaaaggt cgtctgttca agataaccct cgaaaaagcg
    11401 ctattttcca gcccgatggc gtgtgccagc gtcgtggcga atcgtctgaa gcgtcttgag
    11461 agccgtaaag atcataatag ccagagccag atcaacgaac tggagtcatt gctgttggcg
    11521 ctgaataaca ttgatgccag ccagtttagc aaataccagt tgctgctcga caccatccga
    11581 aaagacctcg cctggaaagc caataacacg gaagatcgcc tggtgatctt caccgaaagt
    11641 attaaaacgc tggagttcct cgaacagcaa ctgcgagcgg atctgaaatt gaaagatgac
    11701 cagatcgcca cgctgcgcgg cgatcagggc gatacggtat tgatggaaac cgtagaagcc
    11761 tttggtaaaa cgcagtcgcc gttgcgtctt ctggtttgtt cggatgtggc atctgaaggg
    11821 atcaacctgc atcaccttag ccacaaaatg attcactttg atattccgtg gtcgttgatg
    11881 gtattccagc aacgtaacgg gcgtattgac cgctatgggc aaaaacatca gccccaaatc
    11941 cgctatctgc ttaccgaggc cagcgaacca cagatcaatg gcgatatgcg cgtactggaa
    12001 gtgttgatca acaaagacga acaggcgcag aagaacattg gggacagttc ggagtttacc
    12061 ggcaaattta cccaggaaga ggaagaagag caggttgcag agttcatgat gcaggatgat
    12121 ggtgccagcc tgtttgatca actgctgaac agcaacgtct cggaaagcgc cgaacacgat
    12181 ctgtttggcg aaatatgcag tgcggtttcc agcgatgcct caatggtcac cgaaacagac
    12241 accagcttgt ttgccagcga acaggcgtac tgcgaaagag cattgggtta cctgaaagcc
    12301 agtggtcaaa ctatccagta tgaaaccttg cctgataata ccttgtcgct ggtggcaccg
    12361 gaggagttac gccgccgctt caaccagcta ccgcctgaaa ttgccccgga gaactggcag
    12421 ctctatttaa gtcaggataa aaccgtcatc accgacgcga ttgcgcgcgc tcgcggtgag
    12481 caacatgcct ggcccgatgt gcagtatctc tggcaaatca acccggtagt gcagtggctg
    12541 gacgataaaa tctcttcggc ttttggtcgt catcaggctc cggttatccg gctgccatac
    12601 ctgcttgaac ccgatgaaga tcacttcatt ctttccgggt tattcccgaa ccgtaaatca
    12661 catccgatgg tgaacccgtg gatagtggtg agctttaacc gtgaatcgct aatcggcagt
    12721 cagcctttcg ctgagttttt acaacgtcat ccgcagttga gtaacaagct gactaacagc
    12781 ggcggtaaag atcgtaatca ccaacgccag caggatttac tggaagcggc tattgctcat
    12841 gcccgggagg tattcatcca tgatcggaat gcgtttgaaa cgcacattaa ccagcaactg
    12901 aatgagcatc tgcaaaagct ggacgttttg cgtgggcggc agttgagcca acttgagctg
    12961 gattttgccg ataacaaaca gcaattgtca gtcaagcaga gccgtaaaga gcagagacaa
    13021 cgcgaaatcg aacacaattt tgacagctat atcgaatgga ttgaggacac catgacgact
    13081 gaaaaagaac cctacattca ggtaattgct gttatcaccg gagcggaggg ttaatcatgg
    13141 cgctggtcgg tattaataac gaaaacgaat tttactctaa ccactatttg ggtgaggttt
    13201 tcaccagtga tatccgcgat gtgctggaac cctggatagc ccaggaaaat gcagcgcgtg
    13261 aagcggagcg tgccgctcgt gaacagggca aagacgtgga gccgggatac cgcgctccgt
    13321 ggaaccagtt taacagtctg gcgactgagt ttttccgcaa acttgccgag cacgaaaaac
    13381 agcgtcagat cccgcagcgt ctggccgatc aacgtaatcg ctggcagcca ttgttaaagg
    13441 cgctgggcta cgaaattacg ccacagatcc aaatgctgga tgacgataca ccgctgccgg
    13501 tactggcgcg ttacaacagc actgacggta gcccgtggct gtggattgtt gaagcacacg
    13561 atcaggaaga aggaacgctg gatccgctgg cgctctcctt actgaccgcg caattcccgg
    13621 cggataccga caaacataag cacgacagcc tgcgcaaaaa agccaacggt gaatatcgca
    13681 gctggcagga tttgctctct acggcggtct tcacccaaaa tgaaccgccg cgttttgtgc
    13741 tgctgctcgg taaccgtcag ctattgttgt tggaccgtac taagtgggcg caaaaccgtc
    13801 tgctacgttt tgattttgaa gagattttaa gccgtcgtga aacggatacg ctgaaagcga
    13861 ctgcggtgtt gttacataaa gattctctgc tgccgggcag tggggcacct tatcttgact
    13921 cgctggatga caattcgcac aaacatgcgt ttggtgtttc ggaagatctg aaatatgccc
    13981 tgcgcgaaag cattgagttg ctgggcaacg aagcgatgca ttatctgatc gaccgtggcc
    14041 tggcaaacta tacaggtaat cgtgcggtgg acccggatga actgagccgc gaatgtctgc
    14101 gttacatgta ccgcctgctg ttcctgttct acattgaagc gcgcccggag ctgggttatg
    14161 cgccaatgac cgccaaaacc tatctgcaag gttacagcct ggaaacgttg cgcgatctgg
    14221 agatgatccc gctgaccagc gaagaagatc gcaacgggcg ctacttccac gacagcctga
    14281 atatgctgtt taaactggtg cgcgaaggct acaacggcgg cgtgaaaatg cagagtgacc
    14341 tggagagcgg cgaccggatc accatccata gtcatcagtt cagcgtcccg cgtctggaaa
    14401 gtcatctgtt tgatgccaac aacacgcgca ttcttaaccg cgtggtattc cgtaacgaaa
    14461 ccctgcaaca gattatccag gcgatgtcgt taagccgccc ggccaaaggg cgctttaacc
    14521 gccgcggacg tatttcttat cgccagttgg gtatcaacca gttgggtgcg gtgtatgagg
    14581 cgctgctctc ctatcgcgga ttcttcgcca gcgaagatct ctacgaggtg aagaaagccg
    14641 gggaagagtt taacgagctg gagacgggtt acttcgtcag taaggatgag attagcaaat
    14701 accacgaaga cgagaaggtc tacgagaaag acggcagtct gcgcattcac cgcaaaggca
    14761 gctttatcta ccgtatggcc gggcgcgacc gtgagaaatc tgcctcttat tacaccccgg
    14821 aagtgctgac ccgctcactg gttaaatatg ccctgaaaga actgtttaag gagcagattg
    14881 atcccattag cgatccgcac gccaaagctg atgccatctt aaacctcacc gtgtgcgaac
    14941 cggcgatggg cagcgcggcg ttccttaacg aagccatcaa ccagctggcg gaagcgtatc
    15001 tgttccacaa gcagcaggcg gaaggtcgcc gtattccgca ggatcgttac acccaggagt
    15061 tacagcgggt gaaaatgtac attgccgaca acaacgtttt cggcgtggac ttaaacccgg
    15121 tggcggtgga actggcggaa gtgtcgctgt ggctgaacgc cattagtggc gatgcgtttg
    15181 taccgtggtt tggttaccag ctgcactgcg gtaactcgct ggtgggcgcg cgccgtcagg
    15241 tgttcaacaa gagcgaactg acctacaaaa aagccaaaga tccgagctgg cttaacagcg
    15301 agccggtcga actggcgatg aacacgccgc gtgaagagac gcagattttc cacttcctgc
    15361 tgcccgacgg cggtatggct aactacagcg ataaaactgt taagcagcgt tatccggatg
    15421 acttcaaagc gctggacagc tggcgcaaag agtttattaa aagctttgcc gggcatgaga
    15481 ttgctgatgt gcagcgtatc agcgaaaagg tggaagcact gtggaacacc tatcgccagc
    15541 aacttaaagc agaacgtctg aaaaccgccg acagctaccc ggtgtggccg gcagaaaaca
    15601 gcgagcagac gcgttcttcg ctgagcagta aagatgaaac ctttagcggt cgtcttgaag
    15661 ataacagcgc ctaccagaag ctgcgttggg taatggacta ctggtgcgcg ctgtggttct
    15721 ggccgatcga caaagccgat gagctaccgg atcgcggcac ctggttgttt gagattgaaa
    15781 ccctgcttga cgggattgta atcacggaaa aagtcactga agttgcggag cacaccaccg
    15841 gcgatctgtt tgccgaagaa ggcctgctgc gagaagagtc ttcgctgttt tctgttgctg
    15901 gtcgtctgaa aaccgaggtg ttgttccgtc atttgccgcg tctggcgatt gtcgatgccc
    15961 tgagaaagca gcaccgtttc ttccactggg atctggagtt ctgcgacctg tttgccgagc
    16021 gcggcggttt tgacctgatg ctcggaaacc cgccgtggct gaaagtggaa tggcaggaag
    16081 cgggcgtgct gggtgattac gagccggaat ttgtgctgcg taagctgagc gcctcgaagc
    16141 tggcaacgtt gcgtattgat acctttaacc agatcccggc gctggaagcg gcctggcgca
    16201 gcgagtatga aggctgtgaa gggatgcaaa acttcctgaa tgcgcagcag aactacccgg
    16261 tactgcgcgg ggtgcagacc aacttgtata aatgctttct gccgcaggcc tggcgattag
    16321 gggcgcagaa aggcgtggca ggtttcctgc acccggaagg gatttatgat gacccgaaag
    16381 gcgggcaatt acgtgcggcg gtatatccga ggctgagggc gcattttcag tttcagaatg
    16441 agttaaattt gtttgttgaa gttgatcacc atgcgaagtt tagcagcaat atttattctg
    16501 ctagccctag cacagtggga tttgaacata tatctaattt gtatgctccg caaactattg
    16561 atgcatgttt tgaacattct ggcagtgggg acattcccgg tctcaaagac gagattgaga
    16621 gcgagggaaa attaaaagtt gtatggaaca catctggcca ccgttctcga ttaataagta
    16681 tcgccactca tgagctagaa ttatttgctc gtctatatga cagcgaagga acgccagcct
    16741 ggcaggcacg tttgccagcc ttacatgcta aacaacttgt tgctgtactg gaaaagtttg
    16801 ctaatcagcc gaatagatta ggtgatttgc aggggcagta tttttcaacg gttatgttcg
    16861 atgaaacata tgctcagagg gatgggacaa ttttacggca gactcaattc cctcaagatt
    16921 catcacaatg ggtactgtct ggccctcatt tctttgttgg gacgccgttc tacaagactc
    16981 cgcgcgaaaa ctgtacgctt aacagcgatt atgactgcct ggacttgcta actctgcctg
    17041 acgactatct gccgcgcact aactacattc cggcatgtga tgcacaggag tatgcaaaac
    17101 gtactccatg cgttacatgg actgaactgg ctgaagatga accgaagaag gtaacagatt
    17161 attatcgctt agctatcaga gccatgttgg ctcaatcggg ggaacgtaca ctaattagtg
    17221 ctatttatcc gccagaaata agtcacatga acgcagtacg ttcttactgc tatagctcac
    17281 agaatctgtt actcgaacat tcaggtatgt gtttttcttt accttttgat tttatttgta
    17341 aatctactgg caaggcaaac ttacatcaga tgcttgatgg tttctcatac gtattattca
    17401 atccgagaca aaaggcatta ttatactgct tagtattatc attaaattct gtaaatgatg
    17461 tatatgctgg cctttggcaa tcctgctaca ccccagactt caacacccag cgttggagcc
    17521 gcgatctccc gcagctcccc caggatttct tcgccaaact gaccccagag tggcagcgta
    17581 actgcgcttt acgctctgac tacagtcgtc gtcaggcgct ggtggaaatc gacgtattgg
    17641 tggcgcaggc gctggggtta actctcgaag agctgcttac catttatcgc gttcagttcc
    17701 cggtgatgcg ccagtacgaa gcggatacct ggtacgatca aaacggtcgc attatcttta
    17761 ccccaagcaa agggctggtg ggcgttggct tgcctcgcac cgcgcgtaaa gctgacctga
    17821 aaaacggctt tgtctttaac gtcgacagcc cggagtggac cggcggtgac tgcaccgatc
    17881 aagctatcgg ttgggatgat gtcaaacatc ttaaaaccgg taccgtcagc gtcacctttg
    17941 atgattatac ccgcagcgac gaaggtgagc gccgtaccgt cacctggcag gctccgttta
    18001 tcaagccaga tcgcgaagat gactacaaag tggcctgggc gttctttgca caagataagg
    18061 agagcgcctg atgttgccgt ccgttgtcag tcggcaggtc gccgacagcg ttgccgcctt
    18121 tttacgggcg gcgttcccgc taaacagccc gctgtttaac ggtgagaata acgataatgt
    18181 ctcaatgctg gagcaatttc tggcccagcc ggaaacgctg ctgaaaggac catacctttc
    18241 cgcccagttg ccgtttcgta aaagtgactt accgctgaat tttttcccta atctgacgct
    18301 gccgttcccg ccgcacgcgc atcaggcccg ggcatttcag cgtctgggaa gcgacgcgcc
    18361 acagccgacg ctggtcgcaa ccggtaccgg ctccggtaaa acagaatgtt ttatgttccc
    18421 gctgctcaac cactgcgcgg gcgcgtcaca agccggggtt aaagcgatta tcatctaccc
    18481 gatgaacgcc ctggcaaccg atcaggccag ccgtttcgcc aaaaccatcg ccagcgatcc
    18541 acaactgcat ggcaaggtga ccgtcggcct gtttgtcggc gacagcgaaa tcgaacccag
    18601 caaaaaaatg tcggcagaaa aggtcattac ctgtaagcac acgctgcgcg aaagcccgcc
    18661 ggacatcctg ttgaccaact acaaaatgct cgactacctg ctgatgcgcc ctggcgatca
    18721 gccgctctgg cgttacaacc agcccggttc attgcgttac ttggtcgtgg atgaattgca
    18781 tacgtttgat ggcgcgcaag ggtcggatct ggcctgtctg gtgcgacgtt tgaagcatca
    18841 cataggtgtg gataaccaac gattcgcctg cgtcgggaca tcagctaccg tcggcgacga
    18901 actggggcaa ctgctcgatt acgcaaaaac aatctttgac cagccgttta ccgatgacgc
    18961 cgttattcgc gaagatcgct acacggcagc ggagtacttg cagaattacg ccattgcgta
    19021 cagtcagtat ccggggccgg aggtcgcttc ggccctggac ccgcagagtt acgctacccc
    19081 ggtagcgtat cttaacgggc aaatcccttt gtggttcccc gacagcgact tgcaactccc
    19141 ggacgatctg gagagcgacg acgggcgtga aaagcgtatt gcgctggggt cactgttacg
    19201 ccgccacagc gtgatgcatg tcttactcca tgatctgcaa ggcgggatcc tctctgaacg
    19261 agagtgtctg gaaaatctcc aggtcatgct ggcagaaagc gccgaccatg cccgacgcgt
    19321 gctgcaaagc attctggcac tgatcgcgca ggcacgtctc gaagtaccgg aaacggaaca
    19381 cgatcggcaa aaacgtctcc aggcgaagaa agcgcgtccg gtgctgccgt ttgtgcaact
    19441 gcgctcccag ttgtggcttc gtgagatgcg tcgtctggtc gccagcgtgg cgaaaacgcc
    19501 ccgactggtg tttgccgatg atgtggcggt ggaagatcgc gaacactatt tgccggttat
    19561 ccactgccgt gactgccacg caactggctg ggccagcctg cgtcatgggc aaagcgttca
    19621 gttggaaaca gacctcgacg gcatttatcg ccagttcttt gaaaaagggc gctccatcgt
    19681 ccttgctttc ccggataaca acgaaaaagc ggtcagtggc gcacaccaga aactgtgtcc
    19741 tacctgtctg aaactgaacc gacagtccag cgtccagtgt ggacattgcg gtcatacgga
    19801 actgttgcag gtactgatcc cggatatgct caaagagcgt aaggacgagc tggagtttgc
    19861 taatgaatgc ccgtactgca acagcaaaca ggggatttcg attctggggt cacgggctgc
    19921 cagtctgtcg gcggtgatga ttcaccagct ttatggcagc cagttcaacg aacataaaaa
    19981 gctgattacg ttttctgact cggtacaaga cgccgcgcat cgtgccagct tctttactgc
    20041 ccgtacctgg ccgctgatga ttcgtgggca aatcgccagt gttcttagcg aaaaagagac
    20101 attaccgctg gatgcgttcg cggcgcgtgt ttgccaacaa acgcgtgaac gttcaccgga
    20161 cgatgctcat tttgcagcaa acgtcatcgc accaaatatg cagtggctga atgattatca
    20221 gacgctgaaa aatgaaggca aactggcgga gaactccgat ctgccggagc tggtttccct
    20281 gcgcctcgac tgggaggtgt acagcgaatt taccctgcgc gcgcgtatcg gacgtacgct
    20341 ggaagccacc ggttatgccg ttgccggagt ggataaagat cgcttcaatc cggcaattac
    20401 cgccctgcat caacagcttt gtgaagagct gggtgatgag atgtctggtg tgagcgtcga
    20461 gcaggtagcg cacctggcgc tgggggttat ctggtatcaa cgccaggccg gggccgttat
    20521 gcatccggcg ttcgaaagtt atcgccgtga tggcacgacc tttatgatga cgcaaaaaga
    20581 gcgtacctcg cgctatatgc cttctatcgg tccgaaaacg cgtaaaccgg cttatgcgtc
    20641 gtttgaaaaa attaatggtt tccatcgcct gattggcgcg aagtctaacc ccagttggta
    20701 tcagcattgg atcaatcgca cgctgagtaa cggcaataat ctgtttatca gtagcgttgc
    20761 ggaaacagtg ttacgacgcc tgtttacggc gttgaaaata gccggagtag taaaagattt
    20821 tgacaccaaa gggcgtgaag cctggggact ggtgccttcg gcgctggtgg tgagccgtga
    20881 cgtcgttcag cttaattgct catgttgccg tgaggttgtc cgggctccgg ccgatcaagg
    20941 ctggcagtgg cgtaatgcgc cttgtctctc tttacgttgt gaaggtcgtt accaggaagt
    21001 cgacaatacg gcgacctggc gttgggagaa catggacatc gcgcgcgtac agggcgcaga
    21061 acacacgggg ctgcttagcc gtgaagatcg cgaagtcacc gaaaaatcgt tctaccgcgg
    21121 caaccaaccg tggaatatca acctgttgtc ggcaacaccg acgctggaga tgggtattga
    21181 cgttggggat ctttccaccg ttttactctg ttctgtccca cctgctcagg ccaactactt
    21241 gcagcgtatt ggtcgcgccg gtcgtaaaga tggtaacgcg ttaaacatta ccgtggcaga
    21301 agggaacccg cacgatcagt ttttctttga agaaccactg gagatgatgc agggccaggt
    21361 gcaggctcct ggcgtcttcc ttaatgcaac ggctattctc gaacgccagc tggcagcttt
    21421 ttgtatggat aactgggtga aaaccggtgt accagaatcc gctatctgta aaaacgtcaa
    21481 acagatgctg gatgagctgg agttcgggcg taaatcgggt tttccgtata acttactgcg
    21541 atatatcgaa cagcatcatg cggagattgc tgcgcaattt acggcaatct tccccgatct
    21601 tgccgcagaa actcgccaac agctgttgtc ttatcttcag ggcgcgccag gacaacgctc
    21661 gctggtgcag cgtatcgaag aagcgttaaa actgctggtg gaagatcgca aatctttgcg
    21721 ctcacgcatc gataaactca agcgcagtat cgataaactg gagaacgcgc cgcgcgatca
    21781 aaactttgat agcgatatgc gcgagttaac ttcggaacgg caggcgctga tggcgctggt
    21841 caatcaaatc aacaataagc aaacgctgaa tttcctcacg gatgaaggtc tgctgcctaa
    21901 ctacgccttc ccggaagccg gtattacgtt acgttctgtg ctctggcggc gtaaagaagg
    21961 cggggaagca cgtgaatatc aaaacaccac tttcgaatat gaacgcccgg catcaacggc
    22021 gctggctgag ctggctccgc tgaataattt ctatgccggt gggcataaag ttgaaattga
    22081 gcagatagac ctgaaggtct ctgagccgga gaactggcgt atctgtagcc actgtaacta
    22141 cagcgaaaat atcgaccaga cgggcgacca gcataaatat tgcccgaaat gtggcacacc
    22201 ggggtgggcc gatgccggac aaaaaaccac gctgctgaaa cttcgccagg tttatgcccg
    22261 cgccagcgca cgtgatagcc agatcagcga cgagagcgac agccgtgaac ctgctttctt
    22321 ccagcgtcag ctattggtta gtttcgaaaa agaagatgta tcggcggcat acgcgattga
    22381 ggaaggcgaa gttccgtttg gttttgaatt tttaagcaaa gtcaccctgc gggatatcaa
    22441 cttcggcaaa atggcggatg atgccaatga gttgatgatt gccggagaag cgaagaagcg
    22501 aaccgggttt aaagtttgcc tcgggtgcgg catggtgcag cgtcctcgcg atcatgaacc
    22561 acgtcatgat ttgagctgca aatatcgcac ggaaccggaa aaggcgaagt tcgaagatta
    22621 tctctacctc tatcgccagc tggagtcaga agcgttgcgt attctgctcc cggtaagcag
    22681 ttacagtaat gaccgtgtcg tggaggcttc tcttggggcg gcgatccagc tcggtcttaa
    22741 acattacttc aaggggaatg tcgatcacct gaaaggcgtt gtgtaccgcg agccggaaaa
    22801 tgaaggcgaa tcctggcggc agtatctggt gatttacgat accgtcccgg gggggactgg
    22861 ttctcttaaa gaactgatgc gcactcccga caatttgctg aaattacttg aactggccta
    22921 tcaggcaata gtggaatgca gttgcaacaa tgatacgcat aaagatggtt gttatcgttg
    22981 cgtttacgct taccgtgacc gtgggcgcat gaagtatgtc tcccgcgatc aggcgcgtct
    23041 cttgctggcg aaaattttgc aggcaagaga ttcgatacgg gtaattgatt ccatcaaaaa
    23101 catttccctc gaagccatga tgggcagtga gctggaaaaa cggtttatta gctgcctgca
    23161 agaaaataaa aacctgattg tcagtcgcag ttacgcgcat cagggcgctg ggtggattat
    23221 taacacccgc agcgaaccga tgatgagctg gcatcttaaa gctcaggtgg atttaggcgc
    23281 taaagaggcg gtggggatcc cttgtcgccc ggattacgtg ctatatccgc ttatgcagtc
    23341 tgatgccatt aaaccggtgg ctattttcct tgatggcttt gcgttccaca aaaatagcgt
    23401 cgcagaggat gtgcaaaaac gccaggcgat tcgggatagc ggtaatttct gggtgtggac
    23461 agtcacgtgg gccgatctca aagagccagg gcttaaacat gttcaggatg tcctgggact
    23521 tgggcataac ccggatatga aacagcctaa gttttataac ctgtttcatg ataccaactt
    23581 tgcggccctt gaagcgtcgt tccgggaacg caacagtttt gcactgctgc tggattatct
    23641 cgccgatccg ggcggaagaa cgcagttatg gcagagaatg gctgctgcac atgcctgggt
    23701 gtggttggat gtgaagaagt cacaggacgc gggaacgaaa caaaagtatg cctatgaaat
    23761 gcaggaaaac gcgccagctt ggcggctagc agagcttttg ccagacgaac cgtttgtctt
    23821 tggtggttta ctcgatagct gtaactcatc gcagcaattt attgagctgg cttcggtgtt
    23881 accacaacaa gccattaaac cgacgaccag cgtcgcacaa atgcgcagct ggctgcgttt
    23941 gcatatctgt tttgacgatc gctatacgca ggatgacggt tatgaagcgg gactcaatgg
    24001 cttctggcga ctggtaaacc tgttacagtt cctgccggat atgaccttta ccagccgtaa
    24061 agccgtgcat ttaccgcagg aaagcgttga cactaaagtt tcggcaacgc cagaggcagt
    24121 accagagact gatgagagtt gggctgagat tattgagttt ggcttattaa gcgctgaaga
    24181 gatcgcgttg cttcagtctc attctctctc tgcgccgact ctcggctacg aattgcaaaa
    24241 tgatgaaggt gaaattattg cggaagccga tctcgcctgg ccatcacaaa aatgggcatt
    24301 gattattgat aatcaagaat ttattccatt attcacaacc aggggttggc acgttgcatt
    24361 cgggccaatt gacgaaaata cattgcaaca tctgtccgga ggtgacaaat gacggtgcaa
    24421 acgcccaagg tggcactttc tgatggtttt ctgggcgcat tcgcgcggat cccaaaggca
    24481 cagcaaaaaa aggtgcagga attcatctcc aaattccgtc aggacccgac cagtaatgga
    24541 cttaactatg aaaagatcca cgatgcgcgc agtaaaaatg tccattcggt gcgaatcgat
    24601 caaacttatc gcggcattgt tctgaaacct gaacagggtg ccctgtatat gctgatgtgg
    24661 gtggataaac acgacgaagc atatgactgg gcgcggcggc atgattgttc aattcacccg
    24721 gttaccggcg cgattcaggt cattgatatt agctatatca aaccggcagc ggaaacagtg
    24781 gttgataaac cgaaactctt cgctgcttac agcgcggaac agattctggc tctgggtgtg
    24841 ccgcctgtct ttattgatca ggtgatggcg cttaccgatg aagccgggct taatcaactt
    24901 gaaagcataa tgccagcaga agcctgggaa cctctgcact ggctggcaga agggctggat
    24961 taccaggaag tgctggaaga gtttaacgat catcgtgatg agcctgttga taccaacgac
    25021 tttatggagg cgattgaacg aagtaaacgt cgcttccatg tcgttgaaaa tgaacaggag
    25081 ctgttgcaga tgcttaatgc gcctttagaa aaatggcgtg tatttctgca tccgagccag
    25141 cgaaaattgg tggagtcgcc cgctaatggt ccggtacgag tactgggcgg tgcaggtaca
    25201 gggaaaacgg ttgttgcgat gcaccgtgcg cggtggttat ctcagcgtct ggcagataaa
    25261 cccggtaaga aagtactttt cacgaccttt acgcgtaacc tggcggcaga tattcgcgct
    25321 aatctccagc ggttgtgtac gcgagaagag atggcgcgta ttgacgtcgt taacattgat
    25381 gcctggatga gcgaccaact aaaacgccac ggttatgatt tccgcgtggt atacgacagc
    25441 gatgaagggc gacgcaaatg ctggaactat gccttgcagc aggcacctgc cgaactgggg
    25501 ctacctgata atttctatgc tgaagagtgg caacgcgttg tccagccaaa tgcggtttac
    25561 acccgggagg aatacctcaa agtcagccgg gttggacgtg gtacagcgtt atcccgtatt
    25621 caacgggcga aaatctggcc agtctttgaa gaattcagag cacaaatggc gcgtgccaaa
    25681 ctgcgcgaaa tgggcgatgc catgcatgag gctattgttc tgtttaaaga aaagcaggtc
    25741 caactacctt atagcagtat tgtggtcgat gaagctcaag atatcggtgc cccggcattt
    25801 actcttattc gcagcctggt tcccgagtcg cccaacgatc tgtttattgt cggggatggg
    25861 catcagcgta tttatcgcaa taaggtcgtt cttggtcagt gtggtattaa cgtccgtggt
    25921 cgtcgcagta aaaaattgaa gattaactat cggaccacag aagagacgcg tcaatttgct
    25981 gttgggctgt taactggtgt gaaggttgat aacctcgatg gcgaagcgga taccagtaat
    26041 gattatttgt cattactgca cggggaaaag cccatgatta cgcatgcggc agatttcaaa
    26101 gaagaggctg caacgctcgt caagcaaatt caggcattgc tggcgaatca ggttcgttct
    26161 caggatatct gcattactgc gcgtactaaa catgcttgtg accgctacgc atcagcactg
    26221 aatgacgccg gaattgagac atttaattta ggcaatgata gcggtgacag cgatgcacgc
    26281 cctggcgtgc gggttgcaac gatgcaccgt attaaagggc ttgagttcca gtatgtgttc
    26341 ctggtgggga ttaacgaagg cgttgtgccg gaaataaaag cattagcatc cgatgatccc
    26401 gttgaacagc gtgatgcatt atttaatgag cgcgcgttac tgcatgtggc tgcaacccgt
    26461 gccgtaaaag ggctgtttgt ttcatcatct ggtgtcccta gtccgctatt agtggctgac
    26521 tgataatatg tccaaaacag gccaggagcc gaaacgctcc tggtctatat aaccgttctg
    26581 gcaactggcg atatttatat tgcttagctc ccaacagcca gacaatatca acttagaaat
    26641 tatattcgct tatgcggttt gcatattgca cagtacaacc tgttttctct atatctaacg
    26701 taacgtcagg atgtggatga tttttggatg gggtgacaca ctggacaaat atagctggca
    26761 cgaaaaggct ggcatttcca gctgaccaat agtctctgga accatgatga tgataaaata
    26821 aatatcttaa gttttaaacg tatgatgcca gga
//
LOCUS       DAZFBR010000068        26546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFBR010000068 DAZFBR010000000
VERSION     DAZFBR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.068"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..134
                     /locus_tag="ACHJZ2_002971"
     CDS             <1..134
                     /locus_tag="ACHJZ2_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO4569045.1"
                     /translation="IRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            283..792
                     /gene="ybgA"
                     /locus_tag="ACHJZ2_002972"
     CDS             283..792
                     /gene="ybgA"
                     /locus_tag="ACHJZ2_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO4569046.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            789..2207
                     /gene="phrB"
                     /locus_tag="ACHJZ2_002973"
     CDS             789..2207
                     /gene="phrB"
                     /locus_tag="ACHJZ2_002973"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO4569047.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2357..3838)
                     /gene="dtpD"
                     /locus_tag="ACHJZ2_002974"
     CDS             complement(2357..3838)
                     /gene="dtpD"
                     /locus_tag="ACHJZ2_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO4569048.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            4109..4852
                     /gene="ybgI"
                     /locus_tag="ACHJZ2_002975"
     CDS             4109..4852
                     /gene="ybgI"
                     /locus_tag="ACHJZ2_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO4569049.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4875..5531
                     /gene="pxpB"
                     /locus_tag="ACHJZ2_002976"
     CDS             4875..5531
                     /gene="pxpB"
                     /locus_tag="ACHJZ2_002976"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO4569050.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5525..6457
                     /gene="pxpC"
                     /locus_tag="ACHJZ2_002977"
     CDS             5525..6457
                     /gene="pxpC"
                     /locus_tag="ACHJZ2_002977"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO4569051.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6447..7181
                     /gene="pxpA"
                     /locus_tag="ACHJZ2_002978"
     CDS             6447..7181
                     /gene="pxpA"
                     /locus_tag="ACHJZ2_002978"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO4569052.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7217..8008
                     /gene="nei"
                     /locus_tag="ACHJZ2_002979"
     CDS             7217..8008
                     /gene="nei"
                     /locus_tag="ACHJZ2_002979"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO4569053.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKGLLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(8005..9051)
                     /gene="abrB"
                     /locus_tag="ACHJZ2_002980"
     CDS             complement(8005..9051)
                     /gene="abrB"
                     /locus_tag="ACHJZ2_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO4569054.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLEVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9200..10261)
                     /gene="ybgO"
                     /locus_tag="ACHJZ2_002981"
     CDS             complement(9200..10261)
                     /gene="ybgO"
                     /locus_tag="ACHJZ2_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4569055.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10258..10989)
                     /locus_tag="ACHJZ2_002982"
     CDS             complement(10258..10989)
                     /locus_tag="ACHJZ2_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4569056.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKTPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(11004..13463)
                     /locus_tag="ACHJZ2_002983"
     CDS             complement(11004..13463)
                     /locus_tag="ACHJZ2_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4569057.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALTAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWMGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITLLNLNLYANILHQSWWN
                     ADTSTTANITVGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13513..14079)
                     /locus_tag="ACHJZ2_002984"
     CDS             complement(13513..14079)
                     /locus_tag="ACHJZ2_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4569058.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            complement(14466..15749)
                     /gene="gltA"
                     /locus_tag="ACHJZ2_002985"
     CDS             complement(14466..15749)
                     /gene="gltA"
                     /locus_tag="ACHJZ2_002985"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO4569059.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(15955..16062)
                     /locus_tag="ACHJZ2_002986"
     CDS             complement(15955..16062)
                     /locus_tag="ACHJZ2_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569060.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16443..16847
                     /gene="sdhC"
                     /locus_tag="ACHJZ2_002987"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             16443..16847
                     /gene="sdhC"
                     /locus_tag="ACHJZ2_002987"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO4569061.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            16841..17188
                     /gene="sdhD"
                     /locus_tag="ACHJZ2_002988"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             16841..17188
                     /gene="sdhD"
                     /locus_tag="ACHJZ2_002988"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO4569062.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            17188..18954
                     /gene="sdhA"
                     /locus_tag="ACHJZ2_002989"
     CDS             17188..18954
                     /gene="sdhA"
                     /locus_tag="ACHJZ2_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO4569063.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            18970..19686
                     /gene="sdhB"
                     /locus_tag="ACHJZ2_002990"
     CDS             18970..19686
                     /gene="sdhB"
                     /locus_tag="ACHJZ2_002990"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO4569064.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            19987..22788
                     /gene="sucA"
                     /locus_tag="ACHJZ2_002991"
     CDS             19987..22788
                     /gene="sucA"
                     /locus_tag="ACHJZ2_002991"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO4569065.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            22803..24020
                     /gene="odhB"
                     /locus_tag="ACHJZ2_002992"
     CDS             22803..24020
                     /gene="odhB"
                     /locus_tag="ACHJZ2_002992"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO4569066.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            24114..25280
                     /gene="sucC"
                     /locus_tag="ACHJZ2_002993"
     CDS             24114..25280
                     /gene="sucC"
                     /locus_tag="ACHJZ2_002993"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO4569067.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            25280..26149
                     /gene="sucD"
                     /locus_tag="ACHJZ2_002994"
     CDS             25280..26149
                     /gene="sucD"
                     /locus_tag="ACHJZ2_002994"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO4569068.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(26254..26439)
                     /locus_tag="ACHJZ2_002995"
     CDS             complement(26254..26439)
                     /locus_tag="ACHJZ2_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569069.1"
                     /translation="MNIAFVDLLNATVKRWEASASGNDSKFAVVLIRGLRSVAAIHSG
                     CGHAVKPAVRFRPWLTV"
BASE COUNT         6438 a         6621 c         7235 g         6252 t
ORIGIN      
        1 ggatacggca cattgctatt aatattttga cgaatgataa ggtattcaag gcagggttaa
       61 gacgtaagat gcgaaaagca gccatggaca gaaactatct ggcgtcagtc cttgcgggga
      121 gcgggctttc gtaatcttgc cctggtattc gctgctttga tgatgatgta aaatgattgt
      181 ttttgaactg gatataacag cttggtaggg aagaaagcat aaccccacaa aattcattta
      241 ccatcgtcta cacttactcc tgtaaaccgt tcaggagcag taatgaatca gcaacgattt
      301 gatgacagca ctctaatccg tatcttcgcg cttcatgagt tacatcgact gaaagaacat
      361 ggcttaacgc gcggggcgct tctcgattat cacagccgct ataaactcgt atttctggct
      421 cattcccagc cggaataccg caaacttggc ccgttcgtgg ctgatattca ccagtggcaa
      481 aatctggatg atttttacaa ccagtaccgc caacgcgtaa ttgttttgct ttctcatccc
      541 gccaacccgc gcgatcacac caacgttttg atgcacgttc agggttattt tcgcccgcat
      601 attgagtcca cagaacgcca gcagctggct gcgcttatcg acagttatcg ccgtggcgag
      661 caaccgcttc ttgcgccgct gatgcgtatc aaacactata tggcgcttta tccttacgcc
      721 tggctttcag ggcagcgtta tttcgaactt tggccgcgtg tgattaactt gcgccattca
      781 ggagttttat gactacccat ctggtctggt ttcgccagga tttacgtctg catgataatc
      841 tcgcgctggc tgccgcctgc cgcaattcat ctgcacgcct gctggcgttg tatatcgcta
      901 caccacgcca gtgggcggcg cataatatgt cgccgcgtca ggctgaactt atcaatgctc
      961 aactgaacgg gctacaaatc gcgctggcgg aaaaaggcat tcctttattg ttccgtgaag
     1021 tggatgactt tgccgccagc gtcgaaatag ttaaacaggt gtgcgcggaa aacagcgtta
     1081 ctcatctttt ttataactat cagtatgagg tgaatgagcg ggcgcgggat gtgcaggttg
     1141 agagaactct gcgtaacgtg gtgtgtgaag gatttgatga cagcgtgatc cttccgcctg
     1201 gcgcggtgat gaccggcaat catgagatgt acaaagtctt tacgcctttt aagaatgcct
     1261 ggctgaaacg gctgcgggaa gggatgccgg agtgcgtcgc tgcaccaaaa gttcgtagta
     1321 gcggatcgat aaagcccgcg ccatccatta cgctgaatta tcctcgtcag tctttcgata
     1381 ctgcgcattt cccggtggaa gaaaaagcgg cgattgcgca attacgccag ttttgccaga
     1441 acggtgccgg agaatatgag caacaacgag attttccggc agtggaaggc accagtcgtt
     1501 tgtccgccag cctggcaacg ggcgggttat cgcctcgcca gtgtttgcat cgcttgttgg
     1561 cggaacagcc gcaggcgctg gacggtgggg ccggtagtgt ctggcttaat gagctgatct
     1621 ggcgcgagtt ctaccgtcat ctgatgacgt attacccctc gttgtgtaaa cattgtccgt
     1681 ttattgcctg gacggatcgt gtgcagtggc agagcaatcc cgcacattta caggcctggc
     1741 aggaaggcaa aacgggatac ccgattgtcg atgctgccat gcgtcagctt aacagcactg
     1801 gctggatgca taacaggcta cggatgatta cagccagttt tctggtgaaa gatttgttga
     1861 tcgactggcg cgaaggcgag cgatatttca tgtcgcagct gattgatggt gatttggcag
     1921 ccaataacgg tggctggcag tgggcggctt ctacgggtac tgatgcagcg ccgtattttc
     1981 gtattttcaa cccgacaacc cagggcgaga aatttgaccg cgaaggcgag tttatccgcc
     2041 agtggctacc ggaactgcgc aatgtaccgg ggaaatcggt gcatgagccg tggaagtggg
     2101 ctgagaaagc aggtgtgaag ctggattatc cgcaaccgat agtcgagcac aaagaggcga
     2161 gagtacaaac gttggcagcg tatgaggcgg cgcggaaggg gaaataacag acgcgtcagg
     2221 caatcgagcc cagatgccgg atgcggcgtg aacgccttat ccagcctaca aatttgtgca
     2281 aattcaataa attgcaggaa atacgtaggc ctgataagac gcgtcaagcg tcgcatcagg
     2341 catcggtgct ggcctattaa gactccagcg ccagcgcgcg gtttctgaat ttcagcgcct
     2401 gatacagcca aatcatcagt accactccta cacacgccag tgcgccccag gtaatttgat
     2461 caaatacttc aatatatgca ttgatggagt agttgatcgc cccggaagca tcaaacgaag
     2521 cctgcgatgt ctgatcggca atcacgcccg ccagatagtt cgcaatcgcg ccggaaagca
     2581 gcatataaat gccggttaat acgccggtca caccggggat ttcaatgcgc gtaatctgcg
     2641 acatagcaac cgggtcgata aacagttccg caaagcccat caccgccagg cctaatacca
     2701 tcagtggcag agaagagtga ccatacattg cggaccagcg ggcgcttaag gtcagaatgc
     2761 agaatccggc gctcatcagg ccaaggccaa gagcaaattt tccccagatg cgcacggtac
     2821 gattacccgc gacactctct tttaccaccc acgccaggaa cacaccgcac agcataactg
     2881 cgaaggcatt aatcgactgg aacatcgcgg tcggaacggt ataaccaaac atatcgcgat
     2941 taacgaagcg gtcgatataa aggctaatcg agctaccgcc ctgttgtgcg aaggcccaga
     3001 acaacatact gaagaaggtg agcgtcacaa tcagcctcag ctccttccgc tgtttctggt
     3061 tttctgcttt gcgataaatt tttgccagta cacccaggcc aatgatagtc gcgacaatca
     3121 aggcgtatac cgaccattct ttccagaaca gtacggtaat cagcgcaggc gttgccacca
     3181 gcagaaccag cagccatccc cagttcggca gcagaaagtt tgtcgcacgc agtacttttt
     3241 tgttaacgcc gcgggtatgt gtgaaatgac gattgccaca taagaaaata accagacccg
     3301 cgatcatacc caccgccgcc aggccaaagc ccatcgccca actgtactct tcctgggcgt
     3361 agccacaggc aataggtgcg ataatagacc ccacgttacc cgctgcatac atcagcgaga
     3421 aaccgccatc acgacgcgga tcggttggct catacagctc gccgagcagg caactgacat
     3481 tggatttaaa cagaccatag ccacaaacga ttatcgccag ggacagatag aggaatgacg
     3541 gatggatctc acttgcaccc aacaccacat gaccgatcgc catcaataac gcccccagca
     3601 tcaccgccat gcgattgccg agaactttat ccgccaaaaa gccaccgagg attggcgtga
     3661 catacaccag cgaacagtag gcgctaaata actcgtaggc gtgagtatcg ttgtatttta
     3721 gttgattggt gagatagaga atcagcaggg cacgcatgcc gtaaaagcta aaatattccc
     3781 agatttgcag cgcaacgaca tagtaaatag cgcgcggttg tgatgcgtgt ttattcataa
     3841 taattccaca attgacggca acgtcacgta atagttgcgc ggcgtgcttg tgtttcctta
     3901 aagttaaaac tttgatacag atctgattat ttatgcaata tgctgtctga ttgcataaat
     3961 atacattagc tgaagcgtga tgatataaac aatttgcgtc ttttggcgtc gggtttgttt
     4021 cttaatatgt caaaagattg gctatcgacg aacaggcgag gtaacgctat gttaacggtg
     4081 tagctatcag aatacggaag gcgcgaagat gaaaaacacc gaactggaac aactgattaa
     4141 cgaaaaactg aacagcgcgg cgattagtga ttacgcgccg aatggtttgc aggtggaagg
     4201 caaagagacg gtgcaaaaaa ttgttaccgg tgtcactgcc agccaggctt tgctcgatga
     4261 ggcagtgcgt ctgggcgcgg atgcggtcat tgtgcatcat ggctacttct ggaaaggcga
     4321 atctccggtc attcgcggca tgaagcgtaa ccgtttaaaa acgttgctgg cgaatgatat
     4381 caacctgtat ggctggcatt tgccgcttga cgcacatcct gagctgggca ataacgcaca
     4441 gctggcggcg ttactgggga tcacggtcat gggcgaaatt gagccgttgg tgccgtgggg
     4501 cgaactgacc atgcctgtgc cgggactgga gctggcttcc tggattgaag cgcgtctggg
     4561 acgtaagccg ttatggtgtg gcgataccgg acctgaggtg gtccagcgcg tcgcctggtg
     4621 cacgggcggc gggcaaagtt ttatcgatag cgccgcgcgt tttggcgtgg atgcttttat
     4681 tactggcgaa gtttctgaac agaccattca ttcagcccgc gagcagggat tgcattttta
     4741 tgctgcaggt caccatgcca ctgaacgtgg tggtattcgc gcattgagcg agtggctgaa
     4801 tgaaaatacc gatcttgatg tgacctttat tgatattcct aatcctgcat aacgaataat
     4861 cagagggatc gaaagtgcaa cgagcgcgtt gttatctgat aggtgaaacg gcggtagtgc
     4921 tggaactgga accgccggtg acgctggcta gccagaaacg gatctggcga ctggcgcagc
     4981 gtctggtgga tatgccgaat gtggttgaag ccattcccgg catgaacaat atcacggtga
     5041 ttttgcgtaa tcctgagtcg ctggcgctgg atgccataga gcgtttgcaa cgctggtggg
     5101 aggagagcga ggccctggaa cctgagtctc gctttattga aattccggtg gtttacggtg
     5161 gtgcaggcgg accggatttg gcggtggtcg cggcgcattg cgggttgagc gaaaaacagg
     5221 ttgttgaatt gcactcctcc gtggaatacg tggtctggtt tttaggtttt caaccgggct
     5281 tcccgtatct cgggagtttg ccggaacaac tacacacgcc acggcgcgct gaaccgcgct
     5341 tgctcgttcc ggcaggttct gtcgggatcg gcgggccgca gactggtgtt tatccgctgg
     5401 caacgccggg tggctggcag ttgattggtc atacctcact cagcctgttt gatccggcgc
     5461 gtgacgaacc catcttatta cgtccgggag acagcgtgcg ctttgtaccg caaaaggagg
     5521 gagtatgctg aagattattc gtgcgggcat gtataccact gtgcaagatg gcggtcgtca
     5581 cggttttcgc cagtcgggta tcagccactg cggcgcactg gatatgcctg cgttacgcat
     5641 tgctaacctg ctggtgggta atgatgccaa tgcccccgcg ctggagatca cgctcggtca
     5701 gttaacggtt gagttcgaaa ctgatgggtg gtttgctctg acgggtgccg gttgcgaagc
     5761 gcggctggat gataatgccg tctggaccgg ctggcgattg ccgatgaaag caggccagcg
     5821 tttaacgctt aaacgcccgc agcacgggat gcgcagttat ctggcggtcg cgggtggtat
     5881 tgatgttccg ccggtaatgg ggtcatgcag caccgatctc aaagtgggga ttggcgggct
     5941 ggaaggccgt ttactgaagg atggtgaccg actcccgatt ggcaaagcga agcgtgattt
     6001 tatggaagcg cagggcgtta aacagctgct gtggggcaac cgcattcgcg ccttgccggg
     6061 gccggaatat catgagttcg atcgcgcctc gcaggatgca ttctggcgtt cgccctggca
     6121 gcttagctcg caaagtaacc gcatgggcta tcgcttacag gggcaaattt taaaacgcac
     6181 caccgatcgc gaactgttat ctcacggttt gttaccgggc gtggtgcagg tgccgcataa
     6241 cgggcagccc attgtgttga tgaacgacgc acagaccacc ggtggttacc cgcgtattgc
     6301 ctgtatcatt gaggctgata tgtaccatct ggcgcaaatt ccgctcggtc agccgattca
     6361 ttttgtccag tgttcactgg aagaggcact gaaagcgcgg caagatcagc aacgttattt
     6421 cgaacaatta gcgtggcggc tgcacaatga aaattgacct gaacgccgat ctgggcgaag
     6481 gctgcaccag cgacgcagag ctattaacgc tggtttcctc tgccaatatt gcctgtggat
     6541 ttcatgcagg cgatgcgcaa accatgcagg cttgcgtgcg tgaagcaata aaaaatggtg
     6601 tcgcgattgg cgctcatccg agttttcccg acaggaaaaa ttttggtcgc agcgctatgc
     6661 agctgccgcc agaaaccgtt tacgcccaga cgctgtatca aattggcgcg ctggcaacga
     6721 ttacccgtgc gcaaggtggt gtaatgcgtc atgtcaaacc gcacggcatg ttatacaacc
     6781 aggcggcgaa agaggcacaa ctggcagacg ccatcgccag agcggtatac gcttgcgatc
     6841 cagcattggt tctcgttggg ctggcaggaa gcgagctgat tcgtgcaggc aagcaatatg
     6901 gcctgacaac gcgcgaggaa gtgtttgccg atcgcggtta tcaggctgac ggctcgctgg
     6961 tgccgcgaag ccagtcaggc gcgctgattg aagacgaaga acaggcgctt gcgcaaacgt
     7021 tggagatggt gcaacacggc agagtcaaaa gtatcaccgg cgaatgggca acggtcacgg
     7081 cgcaaacggt ctgcctgcat ggcgacggcg agcacgcact ggcgttcgcc cgccgactac
     7141 gctctgcatt tgccgaaaag gggattgttg tcgcagcata accccgatta ataaagaatg
     7201 aaaaaaggat atcaccatgc ctgaaggccc ggagatccgc cgtgcagcgg ataacctgga
     7261 ggcggcgatc aaaggcaaac cactaactga tgtctggttt gccttcccgc agttaaaaac
     7321 ttatcaatca caacttatcg gtcaacacgt tacccatgtg gaaacgcgtg gtaaggggtt
     7381 gttaactcat ttttccaacg acttaacgct ctacagccat aatcagcttt atggcgtctg
     7441 gcgcgtggtt gataccggcg aagagtcgca gaccacgcga gtattgcggg taaaactgca
     7501 aacggctgac aaaaccattc tgctttatag cgcctcggat attgagatgt tgaccccgga
     7561 acaactgacc acgcatccgt ttttacaacg cgttggtccc gatgtgctgg atccgaatct
     7621 gacgccggag gtggtgaaag aacggttatt gtcgccgcgc tttcgtaacc gtcagtttgc
     7681 tggattactg ctcgatcagg cgtttctggc tgggcttggc aattatttgc gtgtggagat
     7741 cctctggcag gttgggttga ctggaaatca taaagcgaaa gacctcaatg cggcgcaact
     7801 ggatgcactc gcacacgcgt tactggatat tcctcggttt tcttacgcta cgcgggggca
     7861 ggtggatgag aataagcatc atggggcgct gtttcgcttt aaggtttttc atcgtgatgg
     7921 cgaactgtgc gaacgctgtg gcggcatcat tgagaaaacc acgctgtcat ctcgcccgtt
     7981 ttactggtgt ccgggctgcc agcactaggc cgtacgtttc ggcgcatagg ttgaaataaa
     8041 ccgcgcaatg gcaggccctg tcagcaaaat actgaacagg cgcagggttt gcatcgccat
     8101 aatgagcgcc atatcggcat tgctccctgc ggcgatgacc gccacggtat caagcccgcc
     8161 ggggctggtg gcgaggtagg cagtcataaa atcaatgtgc ataaaccggg tcagtcccca
     8221 cgccataccc gcacaaatag ccagcagagc aaaaatcgac agcaggattt gtggtagcgg
     8281 gcgtagtgcc cgcagtaaga tttgtttatc gaaaccaagg ccaatccgcc agccaattgc
     8341 catatacgcc atcgccagta gccattccgg tagttcgatg gtgatgagct gaccagactg
     8401 gagcactgcg cccgccagca tcgggatgag catcgtgccg gaaggaagac gcagcaggca
     8461 tcccactgta ccggcaacga ccgcgagcaa aatagtaagc aggagattaa tgctcactgg
     8521 cgggaaccag acgatatgct ggttaaccgc ttcagcgtta tcgcccagca tcattcgcgt
     8581 gaccagaact gcggcccccg cgacaaacag cactcgcaga tactgcataa acgccaccag
     8641 gcggatatct gcgccgtaat cctgcgccat cgcgaccatt gccgccgccc cgccaggcga
     8701 ggagccccag gcaccagtat ttccgggcag tgagctatag cgcaccagta gccatcccac
     8761 gatagcgctg gaaagcaacg tcaccagtaa aatagctaac acaatcggcc agttaacctc
     8821 cagcgtggtc aggatcgaac cggtcaagtt ttgcgcaatc atgcagccaa gaatggcctg
     8881 cgcggcgaga aaagcggagc gggggagttg cagggtaacc ccgcgcatac tgaagatgat
     8941 cccggcgatc atcggtccaa gtaagagtgc cgccgggaga tgtaatccga ggaaaccaat
     9001 agaaaggaga agtgatagca cacataacat tccccactgc aaaactggca tcctttgctc
     9061 cctcattatt ttctctttgg tgtatcgata cagcataaag aatgccgggc atggaaaaaa
     9121 taacggtaat caataaaagc cggaaaattg gcaattaacc gaagaagccc tctccggctg
     9181 gaaagggcaa taggctaaat cagttgtagt taattttgaa aagtaccact gtatcaaagg
     9241 gaccaacgtc gatgggcgta cccggcgtgg atgacaattc tgccgtgtag gttttttcat
     9301 acactaacag gtcgctggta aaatcggcat actcactgta tttgttgaaa gtatagggtg
     9361 aggcattttc atccaacaat cgaagcttca agccattgcc aatgagcagt gatttatctt
     9421 cgtcgataag ggtttcatcg gtataaaacg aagagctgac tttaaaaccg tcggtacact
     9481 ggtcgttttg tgatttggtt gtagttattt taaattgctg cgacatcgta tggcgacgaa
     9541 tatccagcac gttaaatcgg ccaaaatcaa cgacctgggt ttcaggtgag atagcaaagt
     9601 tgacgctgca atccagcacg cggatatttt ccagaccggt aatgtgatat ttcagattct
     9661 tcgcggtagg gtcttcgttc acgctgcctg cgccatcaaa ctgtacgacg atgtaatcgc
     9721 tgagtttgct ctgataatca tggggcggca tggcgcgaac tttaacgtaa agccgcatac
     9781 gcgccacaaa cgtccgcgat ttgtgcataa cggttggatc gccggagcaa agtttatttt
     9841 gccagcccat cgcaatgatc tgctccgggg tgtaaatatc aatattctta ctgtcgatgc
     9901 actgattcgt gtcgatgcgg cttttcccaa ggcttgcgtc atagtccacg ccttcatagg
     9961 ttacgcccaa ttgataatag ggatcctgaa taccggggta agggtttacc caggcgtaga
    10021 cgtgctcgct ttcaaaatta ccgtttgtgt tgttgtcaca gtagaccgga atttttatat
    10081 cgtcagattc ccagatttta tcgccgggtt tggaattggc cggaacggcg aagggcataa
    10141 tagcttccga tttttctaca gcgccgtttg cggtaccaaa atagcagttc agcgccagcg
    10201 ccgcttttag cggcaaaaaa atcagacaga tggcgagcaa cacgcctttg gcgaatctca
    10261 ttttttattc tccaccggct gcaggcggca ttgccgctgg caggagagcg tgttcatacg
    10321 caatgcgccg tagtcgtcca tgtagccaag ataaaactgc ttgccgctat agctgcccgt
    10381 gctggtggtg accgaactga acggtgcgac catcgtgctt ttaaagccag gcagcacgcc
    10441 tttctcatcc cgcccaagat aggcaatagt cagatagtat ccggtcgggg ttttcagggt
    10501 aagctggtta ttctgctggc taacctgtag ctgttgctca acatgatcgc ccattttttt
    10561 gcgtaatgcg gcgggtcgcc agaatagctt aatgcgactt tggacggcga cttgcaggat
    10621 cgcatggccc tcgctgcctt ccgggacagg cggaatttca cgcaggttat agtaaaagag
    10681 agattcgcga tcggtgggta atttcgcggt ggcggcttgt ttcatgatcc gaacctgcga
    10741 agtcgctttt ggctccagtc gctggatagg cggcagggcg acgagaaatt catcgctttt
    10801 ctctcccttt tcattttcta tccatgaata ggcgaggtag gggagtttat cgctgcggtt
    10861 ttccacgcgc aggctggttg ctttatcttt gctattaaag atgatgcgcg tgcggtcagg
    10921 ctgcactgcg ccgtggctgc acatactggc aaccagaaga atcaaaggga atcctttaac
    10981 aaatgtcatc gtcttttcct taattaatga cagggtaaaa tcaggcgctt agtcacatct
    11041 tccagatgtt ccggcagatg aatggcacac gttttctggt cgccccagtg cacggtaaat
    11101 tgttgatttt catcgacccc actcagccat gcgtgaccgt tttcgccaac catcccgacg
    11161 ctgacaccgc cttcagcctg gcgaacatcg gcaccgagcg gcgggaaatg cccgttagca
    11221 tcgctgataa tgacgttgag atctttcccg gcgcgggacg ccagcgattt aaagccgatg
    11281 gcgccttcgg tccaggtttc tttcaccacg ttttcggaaa cggtaacgcc gtcaggcaag
    11341 tcgttcatat tgaccgccac cgtcgtaggc tggtagctgg aaacaaacgg taccacggca
    11401 ataccaaagc ggttggtgta gtcaatattg ccctgaatcg gaatatcgcc cacgccgtcg
    11461 gtgctgacca tcaggcgtgg ttcattggtg gaactgcggc gatggaatgc cgcgccatgt
    11521 tgagtcgcgg taaacgagcc gctccaactg gcactggcgg aagtgtaatc attggcggca
    11581 taggttccgg tcagatccca ttcccccgcc gaactgaggt gttggtaatt gccgctgacc
    11641 tgcgcaccgt tatccgggcg atcggattgt attccagccg acattcccca actgtttcgt
    11701 tcatccaggg tatcgttcca cgacatgcgg tgggtggtag tattactgtt gttctgcatg
    11761 tcataaccca gtcgaccact ttccccaatc ggtaaggaga tcgaaaagta aatctggttg
    11821 tcgcgatctt tatcttcata atgggtggtg ttgaacgagg tcgagacaga aatatctttc
    11881 cagtcgccaa tgtcgacgtt aaaaccgacg gtaatgttgg ccgtggtgga ggtatccgca
    11941 ttccaccagc tctgatgcag gatgttggcg tagagattaa gattcagcag agtaataggc
    12001 tgaccgaagg agaggctgat tgtttgcttt tcatcctgcg cgtcggcgtc attgtatttg
    12061 tgatcaatgt agttagcgta gctgtgaaaa tggcgatcgg agaagcgata ggctgccaat
    12121 gagatggtgc tgttggtcgc atcaacctgt ttgctgtagt taaagcggta gctgtaaccc
    12181 tgctcatcct gttgggtatc aaaatggctg tcggcccagg tcacgtcaaa cgacagcgcc
    12241 cccatccaga gcatattttg cccaataccc agcgcgccgg aacgatagtc atcgcctgcg
    12301 agaagcatgc cgccatatag tgaagtgttg gagagcattc cccaggagac ttcatggcta
    12361 atgaaggttt catcctcggt gtggtgcgac atagaggaac gcgggcgacc ggcagccagt
    12421 ttatagcgca cctggccctg acgcgtcagg aacggggtag aggccgctga aacctggaag
    12481 ttattcaccc gtccatcttc ttcgctcact ttgacatcca gtgtaccttg cactgactga
    12541 ttcaggtcat caataataaa tgggcctggt ggaacttttt tctggtaaat cacgcgacca
    12601 gaatggctta tcgtgaccgt ggcgttagtc tgtgcgatgc cgctgatttg cggcgcgtag
    12661 ccgcgtaact cccaggggag cattcgatcg tcgctggcta acgccgcacc ggtataggag
    12721 aaaccatcaa aaatgttaga gctaaaatcg gtttcgccca gggttaatcg ggaacctatt
    12781 tgcggtaacg ggcggaacag gtaggtccgt gaaatcgccc cagattgttc acggttgtcg
    12841 ccgctatcgg actggctaag ctgataatcg ctgcgcagac gccatgcccc ggcgttgagg
    12901 cccgtggtgc cataggcgtt caggttgttg ctgctgctgc cgctttgtgg gcgataagtg
    12961 ctggcaaaca ggttgtaatc catcaggaag ccaggaatac cgttattcca cgttgagggt
    13021 ggcgtccagt tttctgaatg ccacgccagc catgcctgcg ggattgtaat attgagctgt
    13081 tgatttgcct gatcgaaagt aaagatgatt tcagggaaga cactaaaatc gacacattct
    13141 tttattgccg gtaatttttt acgaaagtca gatttaagtc cgaagagttc gatcagcgat
    13201 tcattgatac aaggaataat tttatcgcca gatttttgcc agcgaatttg ttgcccatta
    13261 ctgattttat tattattaac cgtaacacta acaaagtaat cgccgggagc tacgacgcct
    13321 ttttgattga gcaacgaaat atcaacgcta tcccgaatcg acttatcgag aacgttaagg
    13381 ttaaattcag ctgtcagcgc aggaggggtt accattgcca gacaggaaag aacagagagt
    13441 cgataaatat tcacggcatc catacctgaa caatattcgc aaaaggcggc taatcagccg
    13501 ccttaaaaag gattattggt aatccagaac gtaagttgcg ttagcagtaa cggcccccgc
    13561 ggtaaccgaa actgcgttat ccatctgttc catccaggca aagaagttta acgtttgttc
    13621 tgttgaactt gccaataagt caattgttgg cgcttctgtt cccaatgtaa caaatgattc
    13681 atctttgttc atgagacgta cgccaacgcc ggtagcctcg ccagcactga tgttactaag
    13741 gagcggggtt gcacctgtcg ttttagccgt gctgtcgaag gtcacgccta cctttgagac
    13801 cacgccaccc ttgccatcat cccagcctgc aatatcacag ttgattaagc gaatattgac
    13861 aggttttttc tcgctatgat gttctcgatt tatgtgggat gtggttactt cacccaggtc
    13921 aatgttcata tcaaggtcat cagggtgaat ttcacatggt gcttcaataa cttcaccttt
    13981 aaagtggatt tcaccagatc cttcatctgc agcaaaaaca ggtgaagcga atagtagaga
    14041 tatggccagg gcggccagtg ttttttgtcc tttaaacata catgaatcct tcatgggcta
    14101 tgataaagta tactaattat tccgacagaa tagtggtttt ttgttcatgt aataatatta
    14161 atgttgctac attatgtttg agagtttctc aaagctctcg aatcataaat aaagtgaatt
    14221 aatcctatta cgagtttatt taattgtggg tgctacccaa tgtattctac atcgattggc
    14281 gggataacgg tggtggtgaa tgaaaaatta ggattttatt tagttatcgg atatgtattc
    14341 ttgtaacgtg taattatgta tttgtgtccg attacgtttt tttgtcgata gacataacta
    14401 acgaacaaaa aaaagcccgc cagatattca ggcgggctaa acgatatatg gttcaaatca
    14461 gataattaat gtttaacatc tgatttaaag tctcgttttt catagccggt gtacaactgg
    14521 cgcggacggg cgatcttcat gccgtcactg tgcatttcgc tccagtgagc gatccagcca
    14581 acggtacggg ccatagcgaa gatgacggtg aacatggaag acggaatacc catcgctttc
    14641 aggatgatac cagagtagaa atcgacgttc gggtacagtt tcttctcgat aaagtacggg
    14701 tcgttcagcg cgatgttttc cagctccata gccacttcca gcaggtcatc tttggtgccc
    14761 agctctttca gaacttcatg gcaggtttca cgcattacgg tggcgcgcgg gtcgtaattt
    14821 ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat catttttatc tttcgcacga
    14881 cgaacaaatt ccggaatgtg tttaacggag ctaatttctt ccagcatttt cagcgccgct
    14941 tcgttagcac caccgtgtgc aggcccccac agtgaagcaa tacctgctgc gatacaggca
    15001 aacgggttcg cacccgaaga gccagcggta cgcacggtgg aggtagaggc gttctgttca
    15061 tggtcagcgt gcaggatcag aatacggtcc atagcacgtt ccagaatcgg attaacttca
    15121 tacggttcgc acggcgtgga gaacatcata ttcaggaagt taccggcata ggagagatcg
    15181 ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt aacacatcgc tgccatggtc
    15241 ggcattttcg acagcaggcg gaacgcggca atttcacgat gacgaggatt gttaacatcc
    15301 agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac cacacatgac tgccattgga
    15361 tgtgagtcgc gacggaaagc gtggaacaga cgggtaatct gctcgtggat catggtatga
    15421 cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag tcggtttttc accattcagc
    15481 aggatgtaac aaacttccag gtagttagaa tcggtcgcca gctgatcgat cgggaaaccg
    15541 cggtgcagca aaataccttc atcaccatca ataaaagtaa ttttagattc gcaggatgcg
    15601 gttgaagtga agccagggtc aaaggtgaac acaccttttg aaccgagagt acggatatca
    15661 ataacatctt gacccagcgt gcctttcagc acatccagtt caacagctgt gtccccgttg
    15721 agggtgagtt ttgcttttgt atcagccatt taaggtctcc ttagcgcctt attgcgtaag
    15781 actgccggaa cttaaatttg ccttcgcaca tcaacctggc tttacccgtt tgttatttgg
    15841 ctcgccgctc tgtgaaagag gggaaaacct gggtacagag ctctgggcgc ttgcaggtaa
    15901 aggatccatt gatgacgaat aaatggcgaa tcaagtactt agcaatccga attattaaac
    15961 ttgtctacca ctaataactg tcccgaatga attggtcaat acttccacac tgttacataa
    16021 gttaatctta ggtgaaatac cgacttcata acttttacgc attatatgct tttcctggta
    16081 atgtttgtaa caactttgtt gaatgattgt caaattagat gattaaaaat taaataaatg
    16141 ttgttatcgt gacctggatc actgttcagg ataaaacccg acaaactata tgtaggttaa
    16201 ttgtaatgat tttgtgaacg gcctatactg ccgccaggtc tccggaacac cctgcaatcc
    16261 cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg aagcagtgtt ttgcatgacg
    16321 cgcagttata gaaaggacgc tgtctgaccc gcaagcagac cggaggaagg aaatcccgac
    16381 gtctccaggt aacagaaagt taacctctgt gcccgtagtc cccagggaat aataagaaca
    16441 gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca aagacctgtt aatctggacc
    16501 tacagaccat ccggttcccc atcacggcga tagcgtccat tctccatcgc gtttccggtg
    16561 tgatcacctt tgttgcagtg ggtatcctgc tgtggcttct gggtaccagc ctctcttccc
    16621 ctgaaggttt cgagcaagct tccgcgatta tgggcagctt cttcgtcaaa tttatcatgt
    16681 ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg tattcgccac atgatgatgg
    16741 attttggcta tctggaagaa acattcgaag cgggtaaacg ctccgccaaa atctcctttg
    16801 ttattactgt cgtgctttca cttctcgcag gagtcctcgt atggtaagca acgcctccgc
    16861 attaggacgc aatggcgtac atgatttcat cctcgttcgc gctaccgcta tcgtcctgac
    16921 gctctacatc atttatatgg tcggtttttt cgccaccagt ggcgagctga catatgaagt
    16981 ctggatcggt ttctgcgcct ctgcgttcac caaagtgttc accctgctgg cgctgttttc
    17041 tatcttgatc catgcctgga tcggcatgtg gcaggtgttg accgactacg ttaaaccgct
    17101 ggctttgcgc ctgatgctgc aactggtgat tgtcgttgca ctggtggttt acgtgattta
    17161 tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag tcagagaatt tgatgcagtt
    17221 gtgattggtg ccggcggcgc aggtatgcgc gcggcgctgc aaatttccca gagcggccag
    17281 acctgtgcgc tgctctctaa agtcttcccg acccgttccc ataccgtttc tgcgcaaggc
    17341 ggcattaccg ttgcgctggg taatacccat gaagataact gggaatggca tatgtacgac
    17401 accgtgaaag ggtcggacta tatcggtgac caggacgcga ttgaatatat gtgtaaaacc
    17461 gggccggaag cgattctgga actcgaacac atgggcctgc cgttctcgcg tctcgatgat
    17521 ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa acttcggcgg cgagcaggcg
    17581 gcacgcactg cggcagcggc tgaccgtacc ggtcacgcac tgttgcacac gctttatcag
    17641 cagaacctga aaaaccacac caccattttc tccgagtggt atgcgctgga tctggtgaaa
    17701 aaccaggatg gcgcggtggt gggttgtacc gcactgtgca tcgaaaccgg tgaagtggtt
    17761 tattttaaag cccgcgctac cgtgctggcg actggcggag cagggcgtat ttatcagtct
    17821 accactaacg cccacattaa caccggcgac ggcgtcggca tggctatccg cgcaggcgta
    17881 ccggtgcagg atatggaaat gtggcaattc cacccgaccg gcattgccgg tgcgggcgta
    17941 ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc tgaacaaaca tggcgaacgc
    18001 tttatggagc gttatgcgcc gaacgccaaa gacctggcgg gccgtgatgt ggtggcgcgt
    18061 tcaatcatga ttgaaatccg tgaaggtcgc ggctgtgatg gtccgtgggg gccacacgcg
    18121 aaactgaaac tcgatcacct gggtaaagaa gttctcgaat cccgtctgcc gggtatcctg
    18181 gagctttccc gtaccttcgc tcacgttgat ccggtgaaag agccgattcc ggttatccca
    18241 acctgtcact acatgatggg cggtattccg accaaagtta ccggtcaggc gctgactgtg
    18301 aatgagaaag gcgaagatgt ggttgttccg gggctgttcg cggtaggtga aattgcctgc
    18361 gtatcggtac acggtgctaa ccgtctgggc ggcaactcgc tgctggacct ggtggtcttt
    18421 ggtcgcgcag cgggtctgca tctgcaagag tctatcgccg agcagggcgc attgcgcgat
    18481 gccagcgagt ctgatgttga agcatctttg gatcgtctga accgctggaa caataatcgt
    18541 aacggtgaag atccggtggc gattcgtaaa gcgctgcaag aatgtatgca gcataacttc
    18601 tcggtcttcc gtgaaggtga tgcgatggct aaagggcttg agcagttgaa agtgatccgc
    18661 gagcgtctga aaaatgcccg tctggatgac acttccagcg agttcaacac ccagcgcgtt
    18721 gagtgcctgg aactggataa cctgatggaa acggcgtatg caacggctgt ttctgccaac
    18781 ttccgtaccg aaagccgtgg cgcacatagc cgcttcgact tcccggatcg tgatgatgaa
    18841 aactggctgt gccactccct gtatctgcca gagtcggaat ccatgacgcg ccgaagcgtc
    18901 aacatggaac cgaaactgcg cccggcattc ccgccgaaga ttcgtactta ctaatgcgga
    18961 gacaggaaaa tgagactcga gttttcaatt tatcgctata acccggatgt tgatgatgct
    19021 ccgcgtatgc aggattacac cctggaagcg gaagaaggtc gcgacatgat gctgctggat
    19081 gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt tccgccgctc ctgccgtgaa
    19141 ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga atggtctggc ctgtattacc
    19201 ccgatttcgg cactcaacca gccgggcaag aagattgtga ttcgcccgct gccaggttta
    19261 ccggtgatcc gcgatttggt ggtagacatg ggacaattct atgcgcaata tgagaaaatt
    19321 aagccttacc tgttgaataa tggacaaaat ccgccagctc gcgagcattt acagatgcca
    19381 gagcagcgcg aaaaactcga cgggctgtat gaatgtattc tctgcgcatg ttgttcaacc
    19441 tcttgtccgt ctttctggtg gaatcccgat aagtttatcg gcccggcagg cttgttagcg
    19501 gcatatcgtt tcctgatcga tagccgtgat accgagactg acagccgcct cgacggtttg
    19561 agcgatgcat tcagtgtatt ccgctgtcat agcatcatga actgcgtcag tgtatgtccg
    19621 aaggggctga acccgacgcg cgccatcggc catatcaagt cgatgttgtt gcaacgtaat
    19681 gcgtaaaccg taggcctgat aagacgcgca agcgtcgcat caggcaacca gtgccggatg
    19741 cggcgtgaac gccttatccg gcctacaagt cattacccgt aggcctgata agcgcagcgc
    19801 atcaggcgta acaaagaaat gcaggaaatc tttaaaaact gcccctgaca ctaagacagt
    19861 ttttaaaggt tccttcgcga gccactacgt agacaagagc tcgcaagtga accccggcac
    19921 gcacatcact gtgcgtggta gtatccacgg cgaagtaagc ataaaaaaga tgcttaaggg
    19981 atcacgatgc agaacagcgc tttgaaagcc tggttggact cttcttacct ctctggcgca
    20041 aaccagagct ggatagaaca gctctatgaa gacttcttaa ccgatcctga ctcggttgac
    20101 gctaactggc gttcgacgtt ccagcagtta cctggtacgg gagtcaaacc ggatcaattc
    20161 cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag acgcttcacg ttactcttca
    20221 acgatctccg accctgacac caatgtgaag caggtaaaag tcctgcagct cattaacgca
    20281 taccgcttcc gtggtcacca gcatgcgaat ctcgatccgc tgggactgtg gcagcaagat
    20341 aaagtggccg atctggatcc gtctttccac gatctgaccg aagcagactt ccaggagacc
    20401 ttcaacgtcg gttcatttgc cagcggcaaa gaaaccatga aactcggcga gctgctggaa
    20461 gccctcaagc aaacctactg cggcccgatt ggtgccgagt atatgcacat taccagcacc
    20521 gaagaaaaac gctggatcca acagcgtatc gagtctggtc gcgcgacttt caatagcgaa
    20581 gagaaaaaac gcttcctaag cgaactgacc gccgctgaag gccttgaacg ttacctcggt
    20641 gcaaaattcc ctggcgcaaa acgcttctcg ctggaaggcg gtgacgcgtt aatcccgatg
    20701 cttaaagaga tgattcgcca cgctggcaac agcggcaccc gcgaagtggt tctcgggatg
    20761 gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg gtaaaaaacc gcaagacttg
    20821 ttcgacgagt ttgccggtaa acataaagaa cacctcggca cgggtgacgt gaaataccac
    20881 atgggcttct cgtctgactt ccagaccgat ggcggcctgg tacatctggc gctggcgttt
    20941 aacccgtctc accttgagat tgtcagcccg gtcgttatcg gttctgttcg tgcccgtctg
    21001 gacagacttg atgagccgag cagcaacaaa gtgctgccaa tcaccattca cggtgacgcc
    21061 gcagtgaccg ggcagggcgt ggttcaggaa actctgaaca tgtcgaaagc gcgtggttat
    21121 gaagttggcg gtacggtacg tatcgttatc aacaaccagg ttggcttcac cacctctaac
    21181 ccgctggatg cccgttcaac gccgtactgt actgatatcg gtaagatggt tcaggcaccg
    21241 atttttcacg ttaacgcgga tgatccggaa gccgttgcct ttgtgacccg tctggcgctc
    21301 gatttccgta acacctttaa acgtgatgtc ttcatcgacc tggtgtgcta ccgccgtcac
    21361 ggccacaacg aagccgacga gccgagcgca acccagccgc tgatgtatca gaaaatcaaa
    21421 aaacatccga cgccgcgcaa aatctatgct gacaagctgg agcaggaaaa agtggcgacg
    21481 ctggaagatg ccaccgagat ggttaacctg taccgcgatg cgctggatgc tggcgattgc
    21541 gtagtggcag agtggcgtcc gatgaacatg cactctttca cctggtcgcc gtacctcaac
    21601 cacgaatggg acgaagagta cccgaataaa gttgagatga agcgcctgca ggagctggcg
    21661 aaacgcatca gcacggtgcc ggaagcggtt gaaatgcagt ctcgcgttgc caaaatttat
    21721 ggcgatcgcc aggcgatggc tgccggtgag aaactgttcg actggggcgg cgcggaaaac
    21781 ctcgcttacg ccacgctggt tgacgaaggc attccggttc gcctgtcggg tgaagactcc
    21841 ggtcgcggta ccttcttcca ccgccacgcg gtgatccaca accagtctaa cggttccact
    21901 tacacgccgc tgcaacacat ccataacggc cagggcgcgt tccgtgtctg ggactccgta
    21961 ctttctgaag aagcagtgct ggcgtttgaa tacggttatg ccaccgcaga accacgcact
    22021 ctgactatct gggaagctca gttcggtgac ttcgccaacg gtgcgcaggt ggttatcgac
    22081 cagttcatct cctctggcga acagaaatgg ggccggatgt gtggtctggt gatgttgctg
    22141 ccgcacggtt acgaagggca ggggccggag cactcctccg cgcgtctgga acgttatctg
    22201 caactttgtg ctgagcaaaa catgcaggtg tgcgtaccgt ctaccccggc acaggtttac
    22261 cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc cgctggtcgt gatgtcgccg
    22321 aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg aagaactggc gaacggcacc
    22381 ttcctgccag ccatcggtga aatcgacgag cttgatccga agggcgtgaa gcgcgtagtg
    22441 atgtgttctg gtaaggttta ttacgacctg ctggaacagc gtcgtaagaa caatcaacac
    22501 gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc cgcataaagc gatgcaggaa
    22561 gtgttgcagc agtttgctca cgtcaaggat tttgtctggt gccaggaaga gccgctcaac
    22621 cagggcgcat ggtactgcag ccagcatcat ttccgtgaag tgattccgtt tggggcttct
    22681 ctgcgttatg caggccgccc ggcctccgcc tctccggcgg tagggtatat gtccgttcac
    22741 cagaaacagc aacaagatct ggttaatgac gcgctgaacg tcgaataaat aaaggataca
    22801 caatgagtag cgtagatatt ctggtccctg acctgcctga atccgtagcc gatgccaccg
    22861 tcgcaacctg gcataaaaaa cccggtgacg cagtcgtacg tgatgaagtg ctggtagaaa
    22921 tcgaaactga caaagtggta ctggaagtac cggcatcagc agacggcatt ctggatgcgg
    22981 ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat ccttggtcgc ctgcgtgaag
    23041 gcaacagcgc cggtaaagaa accagcgcca aatctgaaga gaaagcgtcc actccggcgc
    23101 aacgccagca ggcgtctctg gaagagcaaa acaacgatgc gttaagcccg gcgatccgtc
    23161 gcctgctggc tgaacataac ctcgacgcca gcgccattaa aggcaccggt gtgggtggtc
    23221 gtctgacccg tgaagatgtg gaaaaacatc tggcgaaagc tccggcgaaa gagtctgcac
    23281 cggcagcggc tgctccggcg gcgcaaccgg ctctggctgc acgtagcgaa aaacgtgtcc
    23341 cgatgactcg cctgcgtaag cgtgtggcag agcgtctgct ggaagcgaaa aactccaccg
    23401 ccatgctgac cacgttcaac gaagtcaaca tgaagccgat tatggatctg cgtaagcagt
    23461 acggtgaagc gtttgaaaaa cgccacggca tccgtctggg ctttatgtcc ttctacgtga
    23521 aagcggtggt tgaagccctg aaacgttacc cggaagtgaa tgcttctatc gacggcgatg
    23581 acgtggttta ccacaactat ttcgacgtca gcatggcggt ttctacgccg cgcggcctgg
    23641 tgacgccggt actgcgtgat gtcgataccc tcggcatggc agacatcgag aagaaaatca
    23701 aagagctggc agttaaaggc cgtgacggca agctgacggt tgaagatctg accggtggta
    23761 acttcaccat caccaacggt ggtgtgttcg gttccctgat gtctacgccg atcatcaacc
    23821 cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga tcgtccgatg gctgtgaatg
    23881 gtcaggttga gatcctgccg atgatgtacc tggcgctgtc ctacgatcac cgtctgatcg
    23941 atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga gttgctggaa gatccgacgc
    24001 gtctgctgct ggacgtgtag tagtttaagt ttcacctgca ctgtagaccg acgtggatag
    24061 ggtgtcggtc tacggtttaa aagataacga ttactgaagg atggacagaa cacatgaact
    24121 tacatgaata tcaggcaaaa caactgtttg cccgctatgg cttaccagca ccggtgggtt
    24181 atgcctgtac tactccgcgc gaagcagaag aagccgcttc aaaaatcggt gccggtccgt
    24241 gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa agcgggcggt gtgaaagttg
    24301 taaacagcaa agaagacatc cgtgcttttg cagaaaactg gctgggcaag cgtctggtaa
    24361 cgtatcaaac agatgccaat ggccaaccgg ttaaccagat tctggttgaa gcagcgaccg
    24421 atatcgctaa agagctgtat ctcggtgccg ttgtagaccg tagttcacgt cgcgtggtct
    24481 ttatggcctc caccgaaggc ggcgtggaaa tcgaaaaagt ggcggaagaa actccgcacc
    24541 tgatccacaa agttgcgctt gatccgctga ctggcccgat gccgtatcag ggacgcgagc
    24601 tggcgttcaa actgggtctg gaaggtaaac tggttcagca gttcaccaaa atcttcatgg
    24661 gcctggcgac cattttcctg gagcgcgacc tggcgctgat cgaaatcaac ccgctggtca
    24721 tcaccaaaca gggcgatctg atttgcctcg acggcaaact gggcgctgat ggcaacgccc
    24781 tgttccgcca gcctgatctg cgcgaaatgc gtgaccagtc gcaggaagat ccgcgtgaag
    24841 cacaggctgc acagtgggaa ctgaactacg ttgcgctgga cggtaacatc ggttgtatgg
    24901 ttaacggcgc aggcctggcg atgggtacga tggacatcgt taaactgcac ggcggcgaac
    24961 cggctaactt cctggacgtt ggcggcggcg caaccaaaga acgtgtaacc gaagcgttca
    25021 aaatcatcct ctctgacgac aaagtgaaag ccgttctggt taacatcttc ggcggtatcg
    25081 ttcgttgcga cctgatcgct gacggtatca tcggtgcggt agcggaagtg ggtgttaacg
    25141 taccggtcgt ggtacgtctg gaaggtaaca acgccgaact cggcgcgaag aaactggctg
    25201 acagcggcct gaatattatt gcagcaaaag gtctgacgga tgcagctcag caggttgttg
    25261 ccgcagtgga ggggaaataa tgtccatttt aattgataaa aacaccaagg ttatctgcca
    25321 gggctttacc ggtagccagg ggactttcca ctcagaacag gccattgcat acggcactaa
    25381 aatggttggc ggcgtaaccc caggtaaagg cggtactacc cacctcggcc tgccggtgtt
    25441 caacaccgtg cgtgaagctg ttgctgctac tggcgctacc gcttctgtta tctacgtacc
    25501 agcaccgttc tgcaaagact ccattctgga agccatcgac gcaggcatca aactgattat
    25561 caccatcact gaaggcatcc cgacgctgga tatgctgacc gtgaaagtga agctggatga
    25621 agcaggcgtt cgtatgatcg gcccgaactg cccaggcgtt atcactccgg gtgaatgcaa
    25681 aatcggtatt cagcctggtc acattcacaa accgggtaaa gtgggtatcg tttcccgttc
    25741 cggtacactg acctatgaag cggttaaaca gaccacggat tacggtttcg gtcagtcgac
    25801 ctgtgtcggt atcggcggtg acccgatccc tggctctaac tttatcgaca ttctcgaaat
    25861 gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc ggtgagatcg gcggtagcgc
    25921 tgaagaagaa gcagctgcgt acatcaaaga gcacgttacc aagccagttg tgggttacat
    25981 cgctggtgtg actgcgccga aaggcaaacg tatgggccac gcgggtgcca tcattgccgg
    26041 tgggaaaggg actgcggatg agaaattcgc tgctctggaa gccgcaggcg tgaaaaccgt
    26101 tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt ctgaaataaa tatctgtaat
    26161 aagaaatagc cctcgccgct tccctctaca ggaatggcga agggctgtcg gtttcgacat
    26221 ggttggccat cgtatgatgg ccttttttat gccttaaacg gttaaccacg gtcgaaatct
    26281 gactgcaggc ttgactgcat gaccacagcc actgtggatt gccgcaaccg accgcaagcc
    26341 acgaataaga acgacggcga atttcgaatc gttacctgat gcactggctt cccagcgttt
    26401 gaccgtagcg ttaagtaaat caacaaaagc gatgttcacc gcttttcgtc aagtcgtgcc
    26461 atttgtcagc caggatcggg tctttacccc ggatattgca gcatcgcaaa aattcttaga
    26521 gggatatgct ataaatcaaa aataaa
//
LOCUS       DAZFBR010000069        26068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFBR010000069 DAZFBR010000000
VERSION     DAZFBR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.069"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ACHJZ2_002996"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ACHJZ2_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO4569070.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ACHJZ2_002997"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ACHJZ2_002997"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO4569071.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(938..1405)
                     /locus_tag="ACHJZ2_002998"
     CDS             complement(938..1405)
                     /locus_tag="ACHJZ2_002998"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO4569072.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHJZ2_002999"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHJZ2_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO4569073.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ACHJZ2_003000"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ACHJZ2_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO4569074.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ACHJZ2_003001"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ACHJZ2_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO4569075.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ACHJZ2_003002"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ACHJZ2_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO4569076.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ACHJZ2_003003"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ACHJZ2_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO4569077.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHJZ2_003004"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHJZ2_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO4569078.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ACHJZ2_003005"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ACHJZ2_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO4569079.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHJZ2_003006"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHJZ2_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO4569080.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ACHJZ2_003007"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ACHJZ2_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO4569081.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHJZ2_003008"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHJZ2_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO4569082.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHJZ2_003009"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHJZ2_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO4569083.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ACHJZ2_003010"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ACHJZ2_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO4569084.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ACHJZ2_003011"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ACHJZ2_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO4569085.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ACHJZ2_003012"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ACHJZ2_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO4569086.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHJZ2_003013"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHJZ2_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO4569087.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHJZ2_003014"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHJZ2_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO4569088.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ACHJZ2_003015"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ACHJZ2_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO4569089.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ACHJZ2_003016"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ACHJZ2_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO4569090.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHJZ2_003017"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHJZ2_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO4569091.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHJZ2_003018"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHJZ2_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO4569092.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ACHJZ2_003019"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ACHJZ2_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO4569093.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ACHJZ2_003020"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ACHJZ2_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO4569094.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHJZ2_003021"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHJZ2_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO4569095.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHJZ2_003022"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHJZ2_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO4569096.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHJZ2_003023"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHJZ2_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO4569097.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHJZ2_003024"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHJZ2_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO4569098.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHJZ2_003025"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHJZ2_003025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO4569099.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHJZ2_003026"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHJZ2_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO4569100.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ACHJZ2_003027"
     CDS             15545..15913
                     /locus_tag="ACHJZ2_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO4569101.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ACHJZ2_003028"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ACHJZ2_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO4569102.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ACHJZ2_003029"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ACHJZ2_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO4569103.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHJZ2_003030"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHJZ2_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO4569104.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHJZ2_003031"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHJZ2_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO4569105.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHJZ2_003032"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHJZ2_003032"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO4569106.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHJZ2_003033"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHJZ2_003033"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO4569107.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHJZ2_003034"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHJZ2_003034"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO4569108.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="ACHJZ2_003035"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="ACHJZ2_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO4569109.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRTAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /gene="smg"
                     /locus_tag="ACHJZ2_003036"
     CDS             22590..23063
                     /gene="smg"
                     /locus_tag="ACHJZ2_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO4569110.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="ACHJZ2_003037"
     CDS             23092..23634
                     /locus_tag="ACHJZ2_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO4569111.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHJZ2_003038"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHJZ2_003038"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO4569112.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="ACHJZ2_003039"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="ACHJZ2_003039"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO4569113.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="ACHJZ2_003040"
     CDS             25031..25288
                     /locus_tag="ACHJZ2_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO4569114.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="ACHJZ2_003041"
     CDS             complement(25264..25818)
                     /locus_tag="ACHJZ2_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO4569115.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6689 a         6179 c         6762 g         6438 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaatgatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaataattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttctg cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaagac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggc aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgc gacggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt cgaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cataaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttctgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgtt acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgccgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgccatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcaa cggagggaag caatccagct tctggatatc ctcaaaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tgaataaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctggagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact ggcaggatta cactcacgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgcgt agagaaaaat
    16501 aagaagggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggtc ttctgctttc aggggcttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagtg gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgcaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcctcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag cgaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gtacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggttcgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag ggttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttctggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccact tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg catgcgggaa acctttcatt
    19261 cctgcttcat ccagcaatgc aagccaggtg tcgcgggaat gatgcgtacg attaatacgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggtatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gttaatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attgacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttag aagagtggac tatcagacca gacggttgcc cggaacaaac
    19921 cattcccgac gagagttcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aacgagagca ggttcaggat gccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgcct
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagta tcagcagctt
    21541 gtacggcgat gatatggtcc gtacagctca ctggctggca agacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 gcggaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggtgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagca
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtagcgc ataaagcagc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 ccgccgtcat gcccgactgg ctgccagtct gcttgaacag gggggcgctc tcgtctcgga
    22081 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgtagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgaga agtttttgcc ttgccaggtc caatagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg
    22741 cggattatca ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc
    22801 gtatttatac ccctgaggag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt
    22861 tccttgagca aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taccgcagag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt
    22981 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catatcgtca aagttctgga ggggcaggtt
    23281 tgccctgcat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcattaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgtc cccaatgccg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgtgca
    23881 gcgtgaaacc attttttccc gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacgcg cagcgttggc tggtgctgtt aacaccctca
    24481 agcggttaga agatggacgc ttgctgggtg acaataccga tggtgtaggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgtcctg gtttacgtat tctgctcatc ggcgcaggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
    24661 atcggacggt atcccgagcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 aggcgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgccacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc
    24901 gaggctcaaa gcctactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgtg cgatctctgg
    25141 cgatagcctg gcgtatcgct ttacaggaga tacgccagag cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacttgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25321 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgaatgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaagatg
    26041 gtcgtaacaa cgaagagaaa aaatcctg
//
LOCUS       DAZFBR010000070        25982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFBR010000070 DAZFBR010000000
VERSION     DAZFBR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.070"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            316..1716
                     /locus_tag="ACHJZ2_003042"
     CDS             316..1716
                     /locus_tag="ACHJZ2_003042"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO4569116.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            1872..3407
                     /gene="gadC"
                     /locus_tag="ACHJZ2_003043"
     CDS             1872..3407
                     /gene="gadC"
                     /locus_tag="ACHJZ2_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO4569117.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3538..4857
                     /locus_tag="ACHJZ2_003044"
     CDS             3538..4857
                     /locus_tag="ACHJZ2_003044"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO4569118.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            5234..6616
                     /gene="dosC"
                     /locus_tag="ACHJZ2_003045"
     CDS             5234..6616
                     /gene="dosC"
                     /locus_tag="ACHJZ2_003045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO4569119.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            6641..9030
                     /gene="dosP"
                     /locus_tag="ACHJZ2_003046"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             6641..9030
                     /gene="dosP"
                     /locus_tag="ACHJZ2_003046"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            9288..9869
                     /gene="ddpX"
                     /locus_tag="ACHJZ2_003047"
     CDS             9288..9869
                     /gene="ddpX"
                     /locus_tag="ACHJZ2_003047"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO4569120.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            9883..11433
                     /locus_tag="ACHJZ2_003048"
     CDS             9883..11433
                     /locus_tag="ACHJZ2_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4569121.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            11435..12457
                     /locus_tag="ACHJZ2_003049"
     CDS             11435..12457
                     /locus_tag="ACHJZ2_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4569122.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            12454..13350
                     /gene="ddpC"
                     /locus_tag="ACHJZ2_003050"
     CDS             12454..13350
                     /gene="ddpC"
                     /locus_tag="ACHJZ2_003050"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO4569123.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            13347..14333
                     /locus_tag="ACHJZ2_003051"
     CDS             13347..14333
                     /locus_tag="ACHJZ2_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4569124.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            14326..15252
                     /locus_tag="ACHJZ2_003052"
     CDS             14326..15252
                     /locus_tag="ACHJZ2_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO4569125.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(15308..15739)
                     /gene="osmC"
                     /locus_tag="ACHJZ2_003053"
     CDS             complement(15308..15739)
                     /gene="osmC"
                     /locus_tag="ACHJZ2_003053"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO4569126.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            16084..16299
                     /gene="bdm"
                     /locus_tag="ACHJZ2_003054"
     CDS             16084..16299
                     /gene="bdm"
                     /locus_tag="ACHJZ2_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO4569127.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            16401..16538
                     /gene="sra"
                     /locus_tag="ACHJZ2_003055"
     CDS             16401..16538
                     /gene="sra"
                     /locus_tag="ACHJZ2_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO4569128.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            16695..18392
                     /gene="maeA"
                     /locus_tag="ACHJZ2_003056"
     CDS             16695..18392
                     /gene="maeA"
                     /locus_tag="ACHJZ2_003056"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4569129.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            18526..19536
                     /gene="adhP"
                     /locus_tag="ACHJZ2_003057"
     CDS             18526..19536
                     /gene="adhP"
                     /locus_tag="ACHJZ2_003057"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO4569130.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            19682..19966
                     /locus_tag="ACHJZ2_003058"
     CDS             19682..19966
                     /locus_tag="ACHJZ2_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO4569131.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(20194..20847)
                     /gene="fdnI"
                     /locus_tag="ACHJZ2_003059"
     CDS             complement(20194..20847)
                     /gene="fdnI"
                     /locus_tag="ACHJZ2_003059"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO4569132.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(20840..21724)
                     /gene="fdnH"
                     /locus_tag="ACHJZ2_003060"
     CDS             complement(20840..21724)
                     /gene="fdnH"
                     /locus_tag="ACHJZ2_003060"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO4569133.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(21737..24784)
                     /gene="fdnG"
                     /locus_tag="ACHJZ2_003061"
     CDS             complement(21737..24784)
                     /gene="fdnG"
                     /locus_tag="ACHJZ2_003061"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(24197..24199),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO4569134.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQCISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            25016..25897
                     /gene="yddG"
                     /locus_tag="ACHJZ2_003062"
     CDS             25016..25897
                     /gene="yddG"
                     /locus_tag="ACHJZ2_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO4569135.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6377 a         6603 c         6562 g         6440 t
ORIGIN      
        1 gctattttta tgtaataatt ttataaatgc gttcaaaata ataaccgcgt actaatagtg
       61 atattttaag gtctgatttt tacgtgataa ttcaggaggc acagaatacg cataaaaata
      121 acagcataaa acaccttact accacccaag agctccctac tatattgttt ttcaatgaaa
      181 aaatattatt cacgcaatat ctcaccataa ataacattag gattttgtta tttaaacacg
      241 agtcctttgc acttgcttac tttatcgata aatcctactt ttttaatgcg atccaatcat
      301 tttaaggagt ttaaaatgga taagaagcaa gtaacggatt taaggtcgga actactcgat
      361 tcacgttttg gtgcgaagtc tatttccact atcgcagaat caaaacgttt tccgctgcac
      421 gaaatgcgcg acgatgtcgc atttcagatt atcaatgacg aattatatct tgatggcaac
      481 gctcgtcaga acctggccac tttctgccag acctgggacg acgaaaacgt ccacaagttg
      541 atggatttat ccattaacaa aaactggatc gacaaagaag aatatccgca atctgcagcc
      601 atcgacctgc gttgcgtaaa tatggttgcc gatctgtggc atgcgcctgc gccgaaaaat
      661 ggtcaggccg ttggcaccaa caccattggt tcttccgagg cctgtatgct cggcgggatg
      721 gcgatgaaat ggcgttggcg caagcgtatg gaagctgcag gcaaaccaac ggataaacca
      781 aacctggtgt gcggcccggt gcaaatctgc tggcataaat tcgcccgcta ctgggatgtg
      841 gagctgcgtg agatccctat gcgccccggt cagttgttta tggatccgaa acgcatgatt
      901 gaagcctgcg acgaaaatac catcggcgtg gtgccgactt tcggcgtgac ctacaccggt
      961 aactatgagt tcccgcaacc gctgcacgat gcgctggata aattccaggc cgacaccggt
     1021 atcgacatcg acatgcacat cgacgccgcc agtggtggct tcctggcacc gttcgtcgcc
     1081 ccggatatcg tctgggactt ccgcctgccg cgtgtgaaat cgatcagtgc ttcaggccac
     1141 aaattcggtc tggctccgct gggctgcggc tgggttatct ggcgtgatga agaagcgctg
     1201 ccgcaggaac tggtgttcaa cgttgactac ctcggtggtc agattggtac tttcgccatc
     1261 aacttctccc gcccggcggg tcaggtaatt gcacagtact atgaattcct gcgcctcggt
     1321 cgtgaaggct ataccaaagt acagaacgcc tcttaccagg ttgccgctta tctggcggat
     1381 gaaatcgcca aactggggcc gtatgagttc atctgtaccg gtcgcccgga cgaaggcatt
     1441 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtatgacctc
     1501 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
     1561 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
     1621 ctgttgctgg aagactacaa agcctccctg aaatatctca gtgatcaccc gaaactgcag
     1681 ggtattgccc aacagaacag ctttaaacat acctgataac gtttaacggt aacggtgtcc
     1741 cgaaacgaac ccgtttcggg acaatttcca aagtctgttc actggcatta gcaacggaaa
     1801 atattgttct gaatacgctt cagaacaaaa caggtgcggt tccgacagga ataccgtttt
     1861 agggggataa tatggctaca ttagtacaga caggtaaagc taagcagctc acattactcg
     1921 gcttttttgc cataacggca tcgatggtaa tggctgttta tgaataccct accttcgcaa
     1981 catcgggctt ttcattagtc ttcttcctgc tattaggcgg gattttatgg tttattcccg
     2041 tgggactttg tgctgcggaa atggccaccg tcgacggctg ggaagaaggt ggtgtcttcg
     2101 cctgggtatc aaatactctg gggccgagat ggggatttgc agcgatctca tttggctatc
     2161 tgcaaatcgc cattggtttt attccgatgc tctatttcgt gttaggggca ctctcctaca
     2221 tcctgaaatg gccagcgctg aatgaagacc ccattaccaa aactattgca gcactcatca
     2281 ttctttgggc gctggcatta acgcagtttg gtggcacgaa atacacggcg cgaattgcta
     2341 aagttggctt cttcgccggt atcctgttac ctgcatttat tttgattgca ttagcggcta
     2401 tttatctgca ctccggtgcc cccgttgcta tcgaaatgga ttcgaagacc ttcttccctg
     2461 acttctctaa agtgggcacc ctggttgtat ttgttgcctt cattttgagt tatatgggcg
     2521 tagaagcttc cgcaacccac gtcaatgaaa tgagtaaccc agggcgcgac tatccactgg
     2581 ctatgttact gctgatggtg gcggcaatct gcttaagctc tgttggcggt ttgtctattg
     2641 cgatggtcat tccgggtaat gaaatcaacc tctccgcagg ggtaatgcaa acctttaccg
     2701 ttctgatgtc ccatgtggca ccggaaattg agtggacggt tcgcgtgatc tccgcactgc
     2761 tgttgctggg tgttctggcg gaaatcgcct cctggattgt tggtccttct cgcgggatgt
     2821 atgtcacagc gcagaaaaac ctgctgccag cggcattcgc taaaatgaac aaaaatggcg
     2881 taccggtaac gctggtcatt tcgcagctgg tgattacttc tatcgcgttg atcatcctca
     2941 ccaataccgg tggcggtaac aacatgtcct tcctgatcgc actggcgctg acggtggtga
     3001 tttatctgtg tgcttatttc atgctgttta ttggctacat tgtgttggtt cttaaacatc
     3061 ctgacttaaa acgcacattt aatatccctg gtggtaaagg ggtgaaactg gtcgtggcaa
     3121 ttgtcggtct gctgacttca attatggcgt ttattgtttc cttcctgccg ccggataaca
     3181 tccagggtga ctctaccgat atgtatgttg aattactggt cgttagtttc ctggtggtac
     3241 ttgccctgcc ctttattctc tatgctgttc atgatcgtaa aggcaaagcg aataccggcg
     3301 tcactctgga gccaatcaac agtcagaacg caccaaaagg tcacttcttc ctgcacccgc
     3361 gtgcacgttc accacactat attgtgatga atgacaagaa acactaacta tgtctgaatg
     3421 gttataagat aagggagcga taattcatcg ctcccttttt cgtgcttgct gtcgtattga
     3481 cttaaccggg ataagtacga gaatgagcgc acatctgttt accggaaact agcacatatg
     3541 gatatctgct cccgaaacaa gaaattaacg attagaagac cagcgatact ggttgcactg
     3601 gcacttttac tgtgtagttg taaaagcacg cctccagagt ccatggtgac accaccagcg
     3661 ggttcaaagc caccagccac gacgcaacaa tcgtcacaac cgatgcgtgg catctggctg
     3721 gccacggtgt ctcgcctcga ctggccaccg gtttcctcgg ttaacattag taaccccacc
     3781 agccgggccc gtgtacaaca acaggcgatg atcgacaaac tggatcattt gcaacgtctc
     3841 ggtataaaca cggtcttttt ccaggtcaag ccggacggta ccgccctgtg gccatcgaaa
     3901 attttgccgt ggtccgatct tatgaccggt aagattggtg aaaatccggg ttacgatccg
     3961 ctgcaattta tgctcgacga agcccacaag cgtgggatga aagtacacgc ctggtttaac
     4021 ccctatcgcg tatcggttaa tacgaagccc ggtactatca gggaactgaa tagcactctg
     4081 tctcaacaac cggcgagcgt ctatgtgcaa caccgtgact ggatcagaac gtctggcgat
     4141 cgctttgtcc tcgacccggg catccctgag gttcaggact ggatcacatc aatagtcgca
     4201 gaagtggttt cccgctatcc ggtagatggc gtgcagtttg acgactattt ctatactgag
     4261 tcaccgggtt cacggctaaa tgataacgaa acgtaccgta aatacggagg cgcatttgcg
     4321 tcaaaagcag actggcggcg caacaatact cagcagttaa ttgcaaaggt atcgcacacc
     4381 attaaaagca ttaagccggg agtcgaattt ggtgttagcc cggcaggcgt gtggcgtaac
     4441 cgatcacacg atccgctcgg ttccgatacc cgaggcgcgg cagcctatga cgaatcctac
     4501 gctgacactc gtcgatgggt ggaacaagga ttgctggatt acattgctcc ccaaatttac
     4561 tggccgttct cacggagtgc cgcgcgttat gacgtgttgg caaaatggtg ggcggatgtc
     4621 gttaaaccga ccaggacccg cctgtatatc ggtatcgcct tctataaagt gggtgaacct
     4681 tcaaagatag agccagactg gatgattaac ggcggcgtac cggagctgaa aaagcagctc
     4741 gatcttaacg atgcggtacc agaaattagc ggcaccatct tgttccgtga ggactatctg
     4801 aataaaccgc agactcaaca agcggtcagc tatctgcaaa gtcgttgggg cagttaaact
     4861 cccattctta ctttgtaaca agccggtgct ttacccactg gcttgttacc ctcttgaact
     4921 atcccaccag caatcatgct tactaaagtc aaattaacca cgcaattaac cacgcttagt
     4981 taaacagcca taaatcccct aataaaaatg tatgaatatg aaaaaccata cgatattcgc
     5041 agtcaccgaa aaataattac gatgggctat actcttctga agaacagtag cgtagattga
     5101 aactgtccta agtcgcttat ttcttttcag tatatcttca tatttcagga gaatatggtg
     5161 cagttagtcg atctcgcacg tcgcgtttca ttttgattcc gtctgatttc ccagccttat
     5221 aagggtggga attatggaga tgtattttaa aagaatgaaa gatgagtgga ccggacttgt
     5281 cgagcaggca gatccgccca ttcgtgctaa agccgcggaa attgccgttg cgcatgctca
     5341 ttatctgagt atcgagtttt atcgaattgt ccgcatcgac ccgcatgccg aagaattctt
     5401 gagtaatgaa caagttgagc ggcagttgaa aagtgcgatg gaacgctgga ttattaacgt
     5461 gctttctgcc caggttgacg atgtcgaaag gctaatacaa atccagcata ccgtcgcgga
     5521 agtgcatgcc cgcataggaa ttccggtaga aattgtcgag atggggtttc gggtgctgaa
     5581 aaagatcctc tatccggtaa tcttctcttc ggattattct gccgcagaaa aacttcaggt
     5641 ctaccatttc tcgattaaca gtattgatat cgctatggaa gtgatgaccc gcgcgtttac
     5701 ctttagtgac agtagtgcct caaaagaaga tgaaaactat cgtatcttct cgttactgga
     5761 aaacgccgaa gaagaaaaag aacggcaaat agcctcaata ctttcatggg aaatagatat
     5821 tatctataaa atcctgctgg attctgattt aggcagtagt ttgcctttaa gccaggctga
     5881 ttttggcctg tggtttaacc ataaaggtcg acattatttc agcggcattg ctgaagtggg
     5941 ccatatctct cgtctgattc aggatttcga cggtattttc aatcaaacca tgcgtaacac
     6001 caggaatttg aataacagaa gtctgcgggt gaaattttta ttacagataa gaaataccgt
     6061 atcgcaaatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc acgaagtcgg
     6121 tatggatgta ctgacgaaat tacttaaccg ccgtttcctg ccgactatct tcaaacgcga
     6181 aattgcccat gccaaccgga ccggtacacc gctgtcagtg ctgattattg acgttgataa
     6241 attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc tgcgtaaagt
     6301 ctctcaggcc ttttatgaca acgtccgcag tagtgattat gttttccgct acgggggcga
     6361 tgaatttatc attgttttga ctgaagcttc tgaaaacgaa acgttacgta ccgcagaacg
     6421 tattcgcagt cgggtggaga aaaccaaact gaaagccgca aacggcgaag atattgccct
     6481 ctcactttcc atcggtgccg ccatgtttaa tggtcatcct gactatgagc gcctcattca
     6541 aatagccgat gaagctctgt atatcgccaa aagacgaggt agaaaccgtg ttgaactctg
     6601 gaaagccagt ctttagatgc gccaggatgc agaggtaatc atgaagctaa ccgatgcgga
     6661 taatgccgcc gatggcattt ttttccccgc ccttgagcaa aatatgatgg atgcggtgtt
     6721 aattaacgaa aatgatgaag tgatgttttt caaccccgcc gcagagaagc tctgggggta
     6781 caaacgtgaa gaagtcattg gcaataacat tgatatgctg attccgcggg atttgcgtcc
     6841 tgcgcatcct gaatacattc gtcacaaccg tgaaggcggt aaagcgcgcg tcgaggggat
     6901 gagtcgggag ctgcagctgg agaaaaaaga cggcagtaaa atctggaccc gttttgcgct
     6961 atcgaaagtg agcgccgagg ggaaagttta ttacctggcg ctggtacggg atgccagcgt
     7021 agaaatggcg caaaaagaac agaccaggca attgattatt gccgttgacc atctcgaccg
     7081 accggtgatt gtcctcgatc cggaacgcca tattgtgcag tgcaatcgcg catttaccga
     7141 aatgtttggt tactgcatta gcgaagccag cggtatgcag cctgatacac tcctgaacat
     7201 tcctgaattc cctgccgata accgcattcg tttacaacag ttgctttgga aaaccgcccg
     7261 cgatcaggac gaatttctgc tgttgacgcg caccggtgaa aaaatctgga ttaaagcttc
     7321 tatcagcccg gtttatgacg tgctcgcgca tctgcagaac ctggtaatga ccttctcgga
     7381 tatcaccgaa gaacggcaaa tccgccagct tgaaggcaat attctcgccg ccatgtgcag
     7441 cagcccgcca tttcatgaaa tgggggaaat catttgtcgt aacatcgaat ctgttctcaa
     7501 cgaatcgcat gtttcgctgt tcgcactgcg caacgggatg ccgatacact gggcgtcatc
     7561 ttcgcacggt gcagaagttc aaaatgcgca aagctggtca gcgaccattc gtcagcgtga
     7621 tggcgcgcct gcggggatcc tgcaaattaa aacctcgtca ggagcagaaa ccagcgcctt
     7681 tatcgaacgc gtggcagata tcagccagca tatggccgcg ctggcgctgg aacaggaaaa
     7741 aagccgtcag catattgaac aactcatcca atttgatccg atgaccggtc tgccaaatcg
     7801 caataacctg cacaattacc tcgatgacct ggtcgacaaa gccgtctctc cagtatcggt
     7861 gttgaccata ttcaggatgt gattgatagc cttggctatg cgtgggccga tcaggcattg
     7921 ctggaagtgg tcaatcgctt tcgtgaaaaa ctcaaaccgg atcagtatct ctgtcgtatc
     7981 gaaggtacgc agtttgtcct cgtgagcctg gaaaacgacg tcagtaacat tacccagatc
     8041 gccgatgagc tacggaatgt ggtcagcaag ccgataatga ttgacgataa ccccttcccg
     8101 cttaccttga gtattggcat cagctacgac gtgggtaaaa atcgcgatta cctgctctcc
     8161 actgcccata acgcgatgga ttatattcgc aagaatggcg gtaacggctg gcagttcttc
     8221 agcccggcga tgaacgaaat ggtaaaagag cgtttggttt taggcgcagc gctgaaagaa
     8281 gcgattagca ataaccaact gaaactggtt taccagccgc aaatcttcgc agaaacgggt
     8341 gaactctacg gcatcgaagc ccttgctcgc tggcacgatc cccagcatgg tcatgtgccc
     8401 ccttcacggt ttattcctct cgcagaagag attggtgaaa tcgaaaatat tgggcgctgg
     8461 gtcatcgcgg aagcttgccg tcagttagca gaatggcgta gccagaatat tcatatcccg
     8521 gcgttatccg tgaacttgtc ggcgctgcac tttcgcagta atcagctgcc taatcaggtg
     8581 tctgatgcaa tgcaagcctg gggtattgac ggccaccagc tgacggtaga aatcacggaa
     8641 agcatgatga tggaacacga taccgaaatc tttaagcgca ttcagatcct gcgtgatatg
     8701 ggcgtgggtt tatcggtaga tgattttggc acgggctttt ccggattatc ccgcttagtc
     8761 agtcttccgg taacggaaat caaaattgac aaaagttttg tcgatcgttg tctgacggaa
     8821 aaacgcatcc tggccttact tgaagccatt accagcattg ggcaaagcct caatttaacc
     8881 gtcgtggcgg aaggcgtcga aaccaaagag caatttgaga tgctacgaaa gatccactgt
     8941 cgcgttattc agggatattt cttttcccgc cccctacccg ccgaagaaat tccaggctgg
     9001 atgagcagcg tgttaccgct gaaaatctga caaattcctc tctcccgcac tcgcgggttt
     9061 tcatttaacg tgacactgtc acttaaatac tgtgatttca gctacgattc cgggagattc
     9121 cttccttaac ttcccctctt gcaccaaaac tgcactacgc tgcacaaatg ttgatcaaaa
     9181 gttaaaaaat ataactttaa gccagcattc tggcgcttat cccggcatgg catgagatct
     9241 gcataagcgg aaagcgcagc aatttttgtc ttatacagga accgtttatg tcggatacca
     9301 ccgaactggt tgatttagcc gtgatcttcc ctgatctgga gatcgaattg aaatacgcct
     9361 gcgctgataa catcacaggt aaagctattt accagcaagc gcgttgtctg ttacacaagg
     9421 atgcgattac cgcgctggcg aaaagtatca gtatcgccca gctatcaggc ttacaactgg
     9481 tgatttacga tgcgtatcgc ccacaacaag cacaggcgat gttgtggcaa gcctgcccag
     9541 acccgcaata tgttgttgat gtgacggtcg gttctaatca cagccgtggc acggcgatcg
     9601 acctgacgct tcgtgatgag cacgggaaca tcctggatat gggcgcaggg ttcgatgaaa
     9661 tgcacgagcg ctcccatgcc tatcaccctt ccgtcccgcc cgccgctcag cgcaatcgcc
     9721 tgttgctgaa tgcgataatg actggtggcg gttttgtcgg tatctccagc gaatggtggc
     9781 acttcgaatt accccaggca gcgagttacc ctctgctcgc tgatcaattc acctgtttta
     9841 tatcccctgg cacacagcac gtcagttaat caggaaactg ccatgaagag atcgatatcg
     9901 tttcgtccca cattgctcgc gctcgtcctt gccacaactt tcccggttgc gcacgccgcc
     9961 gtaccgaaag atatgctggt gattggtaag gccgccgatc cacaaaccct cgacccggcg
    10021 gtaacaatag ataataacga ctggacagtg acctacccgt cttatcagcg actggttcag
    10081 tacaaaacgg acggtgataa aggctcaacc gacgttgaag gcgatctggc aagtagctgg
    10141 aaagcgtcta acgatcaaaa agagtggacg ttcaccctga aagataatgc taaatttgcc
    10201 gatggcacac ctgtcactgc cgaagcagta aaactctctt ttgagcggtt actaaaaatc
    10261 ggccaggggc cagcagaagc atttcccaaa gatttaaaga ttgatgctcc cgacgaacat
    10321 acggtgaagt ttacccttag ccagccattt gcaccgttcc tctacacgct ggcgaatgac
    10381 ggtgcatcca ttatcaatcc ggcggtatta aaggaacatg cggcggatga tgcccgcggc
    10441 ttcctcgcgc aaaataccgc cggttccgga ccatttatgc tgaaaagctg gcaaaaaggt
    10501 cagcaattag ttctggtgcc aaatccgcat taccccggca ataaaccgaa ctttaagcga
    10561 gtatcggtaa aaattatcgg tgaaagtgcc tcccgtcgcc tgcagctctc ccgtggcgac
    10621 attgacattg ccgatgcgct gccggtggat caactcaacg ccctgaagca ggaaaacaaa
    10681 gtcaatgtgg cagagtatcc gtcactgcgc gtcacctatc tgtatctgaa taacagcaaa
    10741 gcgccactta atcaggcgga tctgcggcgg gccatttcct ggtctaccga ttatcagggc
    10801 atggttaacg gcattctgag tggtaacgga aaacaaatgc gcggcccgat tccggaaggc
    10861 atgtggggct acgatgcgac ggcaatgcaa tacaaccatg acgaaacgaa agccaaagcc
    10921 gaatgggata aagtgacgag caaacccacc agcctgacgt ttctctactc cgataacgat
    10981 ccgaactggg aaccgatcgc tctggcaaca caatccagcc tcaacaagct gggcatcaat
    11041 gtgaagctgg aaaagctggc gaacgccacc atgcgcgaca gagtgggtaa aggtgattac
    11101 gacattgcga ttggcaactg gagtccggat tttgccgacc cgtatatgtt tatgaattac
    11161 tggtttgagt cagacaaaaa aggtctgccg ggtaaccgct cgttctatga aaacagtgag
    11221 gtcgataagt tactgcgcaa tgcgcttgcg accaccgacc agacgcagcg tacccgagac
    11281 taccagcagg cacagaaaat cgtcattgat gacgctgctt atgtgtacct gttccagaaa
    11341 aactaccaac tggcgatgaa caaagaggtg aaaggctttg tgttcaatcc catgctggaa
    11401 caggtcttca atatcaatac catgagtaaa taacgtgacc ttctggagta ttttacgcca
    11461 acgctgctgg gggctggtgc tcgtggtggc gggcgtctgc gtgattacgt ttattatctc
    11521 gcatctgatc cccggcgatc cggcgcggtt actggcgggt gaccgcgcca gcgatgctat
    11581 cgtggaaaat attcgccagc aactgggact ggaccagcca ctgtatgtac agttttaccg
    11641 ctacgtcagc gacctgtttc atggtgacct gggaacatcc attcgtactg ggcgtccggt
    11701 gctggaagag ttgcgtatat ttttcccggc gacgctggag ctggcttttt gtgccctgct
    11761 gctggcactc ttgattggca tcccgctggg catactctct gcagtctggc gaaatcgctg
    11821 gctggatcat ctggtgcgaa taatggcgat taccggaatc tccacacctg cgttctggct
    11881 tgggctgggc gtcatcgtgc tgttttatgg tcatctgcaa atacttcccg gcggcggaag
    11941 gcttgatgac tggctggatc caccaacgca cgttaccggc ttttatctgc tcgatgcgtt
    12001 gcttgaaggc aacggtgaag tcttcttcaa tgcgttgcaa catctcatct taccggcatt
    12061 aacgctggcg tttgttcacc tggggattgt cgcccgccag atccgctcag cgatgctgga
    12121 acaattgagt gaagactaca ttcgtaccgc ccgggccagc ggcttgcccg gctggtatat
    12181 cgttctatgt tatgcgctac ccaatgcgtt gatcccttcg attaccgtat tggggctggc
    12241 gctgggcgat ttgttgtatg gcgcagtgct caccgaaacc gtttttgcct ggcccggaat
    12301 gggggcatgg gtagtgacat caatacaggc gctcgacttc ccggcagtga tgggctttgc
    12361 cgtcgtggtt tcatttgctt atgtgctggt caacctggtg gtggatttgc tctatttgtg
    12421 gattgatccg cgtatcggac gtggaggtgg tgaatgatgc taagcgagga aacgtccgcc
    12481 gtacgcccac aaaaacaaac gcgatttaac ggtgcaaaac tggtatggat gctgaaaggc
    12541 agtccgctca ccgtgaccgg cgcagtcatc attgtattaa tgctactgat gatgattttt
    12601 tcaccgtggc tggcgacgca tgatcccaac gccattgatt taaccgcccg ccttttgccg
    12661 ccttctgcgg cgcactggtt tggcaccgat gaagtgggac gcgatctgtt cagccgcgta
    12721 ctggtcggca gtcagcaatc aattctcgcc ggattagtcg tggttgccat tgcgggtatg
    12781 attggttcgc tactcggatg tctatccggt gtgcttggcg gacgcgcaga cgccatcatc
    12841 atgcgcatca tggacattat gctgtcgatt ccttcgttgg tactgacaat ggcactggct
    12901 gccgctctcg ggccgagttt gtttaacgcc atgctggcta ttgctattgt gcgaattccc
    12961 ttttatgtgc gcctggcgcg ggggcaagca ttagttgtac gccagtatac ctatgttcag
    13021 gcggcgaaaa cctttggcgc gtctcgttgg catctgatca actggcatat tttacgtaac
    13081 tccctaccgc cgctgatcgt gcaggcatcg ctggatatcg gtagcgcgat tttaatggcc
    13141 gccacgttgg gatttattgg cctgggtgct caacaaccga gtgctgaatg gggcgcgatg
    13201 gtagcaaatg gtcgcaacta tgtactcgat cagtggtggt attgcgcatt tccgggggca
    13261 gcgattttgc ttaccgccgt cgggtttaat ctctttggtg atggtattcg cgatctgctt
    13321 gacccgaaag caggaggaaa gcagtcatga cccaacccgt tctggacatt caacaactgc
    13381 atttgagttt ccccggtttt aacggcgacg ttcacgcgct caacaatgtg tccttgcaga
    13441 ttaaccgcgg tgaaattgtc ggtctggtgg gagaatccgg ctcaggtaaa tcagtcaccg
    13501 caatgctgat tatgcgtctg ctaccgacgg gcagttattg cgtacatcgg gggcagattt
    13561 cactgctggg agacgatgtt cttaacgcgc gggaaaaaca gcttcgtcag tggcgcggcg
    13621 cacgagtggc gatgatcttt caggaaccga tgaccgccct caatccgaca cgtcgaatag
    13681 gtcttcagat gatggacgtg atccgccatc atcaaccaat aagtcgtcgg gaagccagag
    13741 ctaaagcgat tgccctgctg gaagagatgc aaatcccgga taccgtggaa gttatgtcgc
    13801 gctatccgtt tgagctttca ggtggtatgc gccagcgggt aatgattgcg ctggcattct
    13861 cctgcgaacc gcaattgatt attgccgacg aaccgactac ggcgctggac gtcacggtac
    13921 agttgcaggt actgcgtttg cttaaacata aagctcgcga cagtggaact gcggtactgt
    13981 tcatcagcca tgatatggcc gtggtgtcgc aactgtgcga tagcgtttac gtgatgtatg
    14041 ccggaagcgt gattgagagc ggcgtgacgg cagacgttat ccatcatccc cggcatccgt
    14101 ataccattgg tttgctgcaa tgcgcaccag aacatggagt accacgccag ccattacccg
    14161 ccattccagg gacggtacca aatctcaccc atttgcctga cggctgcgct tttcgcgatc
    14221 gttgctatgc ggcaggtgca cagtgtgaaa acgtcccggc gctgacagcg tgtggtgaca
    14281 acaaccagcg ctgcgcctgt tggtatccgc agcaggaggt cataagtgtc tgacacgtta
    14341 ttaacgttac gcgacgtcca tatcaatttc ccggcgcgta aaaactggct tggtaaaact
    14401 acggaacatg ttcatgccat taatggtatt gatttacaga tccgccgtgg tgaaacctta
    14461 gggatcgtcg gcgagtcagg ctgcggcaaa agcaccctcg cacagctttt aatgggtatg
    14521 ctgcaaccga gccacgggca gtacatccgt tctggctcac aacgcattat gcagatgatg
    14581 tttcaggacc cgctctcttc gcttaatccg cgcttaccgg tgtggcgcat catcacagaa
    14641 ccgctctgga tagctaagca tagtagtgaa caacagcggc gagcgttggc agaggagctg
    14701 gctgtgcagg tgggtattcg tccggagtat ctcgaccgcc tgcctcatgc gttctccggc
    14761 gggcagcggc aacgcatcgc cattgccaga gcactctctt cgcagcctga cgtgattgtg
    14821 cttgatgagc caacctctgc gctggatatc tccgtgcagg cgcagatcct caatttactg
    14881 gtaacgctac aggaaaatca cgggctgact tatgtgctga tttcacacaa tgtctcggtg
    14941 atacgtcata tgagcgatcg ggtggcggtg atgtatctcg ggcagattgt ggaattaggg
    15001 gaagcgcaac aggtgctaac ggctccggca catccataca cccgattatt gctggattcc
    15061 ctccccgcca ttgataaacc actggaggaa gaatgggcat tacgtaaaac ggatctgcca
    15121 ggaaaccgca cgttgccaca gggctgtttt ttccgcgaac gttgccccct tgcaaccagc
    15181 ggatgcgaag tccggcaatc attaacgacg cgggctgacg gacgtgagat ccgttgctgg
    15241 cgggcgctgt agagatgcta acaacgcatc aggcattcat gcgcctgacg cgtcatccgg
    15301 caatgcttta cgatttcaac tggtaatcca gcgtaatttc cgctttcagt acctgagaga
    15361 ccgggcatcc tgcttttgct ttctggatga tgccgtcaaa ggtagaggca tcaatacccg
    15421 gcaccgcaac ttcactcttc agtgcgattt tcgtaatcgc aaaaccggca tccactttat
    15481 ccagcgacac atcggcggtg gtatcaatcg atgttggcgt gaatcccgct tcccccagca
    15541 ttaatgaaag cgccattgag aaacatgcgg catgcgctgc gccaatcagt tcttcagggt
    15601 tggttccttt ttcgccttca aaacgcgtgt taaatccata cggctgttgg ttcagcacgc
    15661 cactctcggt ggatactgtt cccttcccgc gtttgatatc gctttcccag tgtgcctgac
    15721 ctttcttatg gattgtcatt gttgctctcc tgtgggctta aaaaggaaat tatagactac
    15781 gtgagaaacc gcacgaggat aagcaggata ttccgaataa aatacggcaa aaatattact
    15841 tctgcactta aattaaattc ccacaataga taaccggaaa tatatatctc aagagagatt
    15901 gcatcaatat taatcaactc atcacaataa taatacctat caatatcatg gggttaaaat
    15961 ataaaaatca tcaaccagga ctaatcttaa caacgaagcg gcaaatattt gccatgctga
    16021 atgtgcttta taaatctgcg atccgtagca gacactataa atacacagac acggagaatc
    16081 actatgttta cttattatca ggcagaaaat tcaacagcag aacccgctct ggttaatgcc
    16141 attgagcaag gccttcgggc agagcacggt gttgtcactg aagatgacat tctcatggag
    16201 ctgaccaaat gggtggaagc ttctgataac gacatcctca gtgatatcta ccagcaaacg
    16261 atcaattatg tggtcagtgg ccagcaccct acgctttaag atgctatgct tgatcggcaa
    16321 cctaatttag gggtttagca cgtgtttctt cgctacggcg atgttgtcct taaaactagc
    16381 aacaggattg aggagttaaa atgaaatcga accgtcaggc acgtcatatt cttggactgg
    16441 accataaaat atctaaccag cgcaaaatag ttaccgaagg tgacaaatcc agcgtggtaa
    16501 ataacccaac cggcagaaaa cgccccgctg aaaagtaatt cataaccatc agtcctcaat
    16561 gacgattaaa caccattgcc tgcgcaatgg tgtttttgtt tttatctgct ttatacttgg
    16621 ggccgacgcc ctggcggtaa agcaaagacg ataaaagccc cccagggatg gatattcaaa
    16681 aaagagtgag tgacatggaa ccaaaaacaa aaaaacagcg ttcgctttat atcccttacg
    16741 ctggccctgt actgctggaa tttccgttgt tgaataaagg cagtgccttc agcatggaag
    16801 aacgccgtaa cttcaacctg ctggggttac tgccggaagt ggtcgaaacc atcgaagaac
    16861 aagcggaacg agcatggatc cagtatcagg gattcaaaac cgaaatagac aaacacatct
    16921 acctgcgtaa catccaggac accaacgaaa ccctcttcta ccgtctggta aacaatcatc
    16981 ttgatgagat gatgcctgtt atttataccc caaccgtcgg cgcagcctgt gagcgttttt
    17041 ctgagatcta ccgccgttca cgcggcgtgt ttatctctta ccagaaccgc cacaatatgg
    17101 acgatattct gcaaaacgtg ccgaaccata atattaaagt gattgtggtg actgacggtg
    17161 aacgtattct ggggcttggt gaccagggca tcggcgggat gggcattccg atcggtaaac
    17221 tgtcgctcta taccgcctgt ggcggcatca gcccggcgta tacccttccg gtggtgctgg
    17281 atgtcggaac gaacaaccaa cagctgctta acgatccgct gtatatgggc tggcgtaatc
    17341 cgcgtatcac tgacgacgaa tactatgaat tcgttgatga atttatccag gctgtgaaac
    17401 aacgctggcc agacgtgctg ttgcagtttg aagactttgc tcaaaaaaat gcgatgccgt
    17461 tacttaaccg ctatcgcaat gaaatttgtt cctttaacga tgacattcag ggcaccgcgg
    17521 cggtaacagt cggcaccctg atcgcagcaa gccgcgcggc aggtggtcag ttaagcgaga
    17581 aaaaaatcgt cttccttggc gcaggttcag cgggatgcgg cattgccgaa atgatcatcg
    17641 cccagaccca gcgcgaagga ttaagcgagg aagcggcgcg gcagaaagtc tttatggtcg
    17701 atcgctttgg cctgctgacc gacaagatgc cgaacctgct gcctttccag accaaactgg
    17761 tgcagaagcg cgaaaacctc agtgacaggg ataccgacag cgatgtgctg tcactgctgg
    17821 atgtggtgcg caatgtaaaa ccagatattc tgatcggcgt ctcaggacag accgggctgt
    17881 ttacggaaga gatcatccgt gagatgcata aacactgtcc gcgtccgatc gtgatgccgc
    17941 tgtccaaccc gacgtcacgc gtggaagcca caccgcagga cattatcgcc tggactgaag
    18001 gtaacgcgct ggtcgccacg ggcagtccgt ttaatccagt ggtatggaaa gataaaatct
    18061 accctatcgc ccagtgtaac aacgccttta ttttcccggg catcgggctg ggtgttattg
    18121 cttccggcgc gtcacgtatc accgatgaga tgctgatgtc ggcaagtgaa acgcttgccc
    18181 agtattcgcc gctggtcctg aacggcgaag gtctggtact accggaacta aaagatattc
    18241 agaaagtctc ccgcgcaatt gcgtttgcgg ttggcaaaat ggcgcagcag caaggcgtgg
    18301 cggtgaaaac gtctgccgaa gctttgcaac aagccattga cgataatttc tggcaagccg
    18361 aataccgcga ctaccgccgt acctccatct aagcctgcgc ccggtagtga aggctaccgg
    18421 gctatttccc tctccctttt tcagatctca tccatactgg gtagtggcga ataaatctca
    18481 tttgcctcac ctgctatgca gaacatcatc cgaaaaggag gaactatgaa ggctgcagtt
    18541 gttacgaagg atcatcatgt tgacgttacg gataaaacac tgcgctcact gaaacatggc
    18601 gaagccctgc tgaaaatgga gtgttgtggt gtatgtcata ccgatcttca tgttaagaat
    18661 ggcgattttg gtgacaaaac cggcgtaatt ctgggccatg aagggattgg tgtggtggca
    18721 gaagtgggtc caggtgtcac ctcattaaaa ccaggcgatc gtgccagcgt ggcgtggttc
    18781 tacgaaggat gcggtcattg cgaatactgt aacagtggta acgaaacgct ctgccgttca
    18841 gttaaaaatg ccggatacag cgttgatggc gggatggcgg aagagtgcat cgtggtcgcc
    18901 gattacgcgg taaaagtgcc agatggtctg gactcggcgg cggccagcag cattacctgt
    18961 gcgggggtca ccacctacaa agccgttaag ctgtcaaaaa ttcgtcccgg gcagtggatt
    19021 gctatctacg gtcttggcgg tctgggtaac ctcgccctgc aatacgcgaa gaatgtcttt
    19081 aacgcgaaag tgatcgccat tgatgtcaat gatgagcagt taaaactggc aaccgaaatg
    19141 ggtgcagatt tagcgattaa ctcacgcacc gaagacgccg ccaaaattgt gcaggagaaa
    19201 accggtggcg ctcacgctgc ggtggtaaca gcagtagcta aagctgcgtt taactcggca
    19261 gttgatgctg tccgtgcagg cggtcgtgtt gtggctgtcg gtctgccgcc ggagtctatg
    19321 agcctggata tcccacgtct tgtgctggat ggcattgagg tggtcggttc gctggtcggc
    19381 acgcgccagg atctaactga agccttccag tttgccgccg aaggtaaagt ggtgccgaaa
    19441 gtcgccctgc gtccgttagc ggacatcaac accatcttta ccgagatgga agaaggcaaa
    19501 atccgtggcc gtatggtgat tgatttccgc cgctaagagg cctttgctgc gactcccatg
    19561 ttcgggtcgc agcatcgcac actctccaac atgaaatggc taaatggatt attgttgcgt
    19621 aggaaggaac aataatgata gtgttcccag agaaggaacg ctactgagag taaagacact
    19681 aatgaaaatg gccaatcatc cccgcccggg ggacattatt caggaatcac tggacgaact
    19741 taatgtcagc ctgcgcgagt ttgccagagc aatggaaatt gctccctcaa cggcaagccg
    19801 attgctgacc ggaaaagcag ctttgacgcc agaaatggca attaaacttt ccgtggtgat
    19861 cggcagttcg ccgcaaatgt ggctgaatct gcaaaacgcc tggagtctgg cagaggctgc
    19921 aaaaacggtg gatgtgtcgc gattacgccg tctggttgcg caataaaaaa tggcgccttt
    19981 tacagcgcca tttgaaactg atgacaaacg caacattgcc tgatgcgcta cgcttatcag
    20041 gcctacacgt tcactgcaat ctattggatt tgaacggttt gtaggccgga taaggcgttc
    20101 acgccgcatc cggcatgaac aaagcctacg ttgtctacaa tatgaaaatg gcgcctttta
    20161 cagcgccatt ttcagcttaa agttcaaaag agattatatc ccttcttcac tctctttttt
    20221 cgcttctgcc ttctcgattt cacgatacca gcgcggatgg tgtttcttcg cccagcgacg
    20281 gctcaccttc ccttcgatca tccctttaat cgatcctttc acccagaatg ccatatacat
    20341 atggatcagg atggcgtgga tcaggatgat acccgcagcc gcgtggatca gcaggctgta
    20401 gcgaacaacc tgcatcggga agtactgcgc aaagtacgga cgccagataa tcaccccggt
    20461 caccagcagc acgaaaatca tgctcatgat cgaccagaac atcatctttt gcccggcgtt
    20521 gtacttaccg acatccgcca ctttatgctc attgcctttc aatacttcga caatgtttaa
    20581 cagccacgga atatctttct tatccgggat gttgtgatgc acaaaacgca caaacataaa
    20641 catcagtgcg acgaaaatcg caatgccgaa gaatgggtgc aaaatgcgtc ccatctgcgg
    20701 cgtaccgaag gtttgcgtca gccattgcag cgtcgggaag aagaacgaaa tcccggacag
    20761 cgccaccagg aagaagcaaa tcaccacggt ccagtgacag gcgcgatcaa taaatttggt
    20821 gcgcacaatc attttcgact tactcatgat gatcctcctc gtcatcgtcc acttccttat
    20881 tcgggccaat accgatgtag tggaaaatca accctgcaaa agtggcaata aagccagccg
    20941 ctgccagcgg tttcaacgcg cctttccaca ggcttaccga ggtgtcgatc ttcggatctt
    21001 tcggcagacc gtgatacagc tccggctgat cggcgtgatg cagcacgtac ataacgtgcg
    21061 taccaccgac cccttccggg ttgtagacgc cagcatgttc gtaaccacgc gctttcagtt
    21121 tcgccacgcg ctgttccgcc agctccagca tctccttctt ggtgccgaag tggatagccc
    21181 cggtcggaca ggttttcaca caagccggtt cctggccgac gctgacgcga tcgacgcaga
    21241 gcgtgcattt atatacccgg ttatcctctt tgttgaggcg cggaatatta aacggacacc
    21301 cggcaatgca gtaaccacag ccgatgcagt tttccgactg gaaatcgaca atcccgttag
    21361 cgtactgaat gattgcaccg gcagacgggc acgccttcag gcagcccgga tcttcacagt
    21421 gcatacagcc atctttacgg atcagccact ccagcttgcc gctctgttcg gtttcgctaa
    21481 agcgcatcac cgtccaggac ttggcgctca gatcggcggg gttatcgtaa accccgacgc
    21541 agtgccccac ttcatcacgg atgtcgttcc actccgaaca cgccacctga caggctttac
    21601 agccgataca ggtggaaacg tcgataagtt ttgcgacttc tgctttgtaa tcacgcacct
    21661 gagaaggcgg cgtgatggag ttagttgcgg accttttgat aatgtcctgc gtttccatag
    21721 ccatctgttc gcccccttac gccttctcga tgttgactaa gaacgcttta tattccggcg
    21781 tttgcgagtt tgcatcaccg acattcggcg tcagagtgtt agctatataa cctttacgcg
    21841 cgacaccctc aaagccccag tggattggaa tacccaccgt ttcaacctgc tgaccattta
    21901 cattcagcgg tttcagacga cgcgttacca cagccaccgc gcggataaag ccacgcttgc
    21961 ttgagacagt gacacgatcg ccattattaa tgcctttcgc cgccgccagc gtttcgctga
    22021 tttccacaaa ctgttccggc tgagcaattg cgttgagcaa cgcgtgcttg gtccaggtgt
    22081 ggaagtgctc ggtcagacga taggtcgtac ccacatacgg gaactgctct tttttaccca
    22141 tccgcagcgc gtcttgttca tacagacgaa ccaccgggtt agaaaccacg ttcggatgca
    22201 gcgggttagt tcccagcggc gtttcaattg gctcgtagtg ttccgggaac ggaccttccg
    22261 ccattttgtt gatggcaaac aggcgtccca tcccttccgg ctgcatgata aacggcccgg
    22321 ttggcgtacc cggtgcggca ttgccgaagt cagggatatc gttacccgtc cacttgctgc
    22381 cgttccactg gatcagcatc cgtttcggat cccacggttt accgttgata tctgccgaag
    22441 cacggttgta gagtacgcga cggttaagcg gccacgccca ggcccatccc agcgtattcc
    22501 ccagaccgga cgggtcggag ttatcgcggt tagccatctg gttgccctgc tctgtccagc
    22561 taccggtgta gatccagcaa gaagatgcgg ttgtaccgtc atcacgcaga tgcgcaaagc
    22621 tactcagcaa ctgacctttc ttcgcaatca gcacgccatt agcatcatag agatcttcca
    22681 gcgcatagcc gttgttctct ttagctactt cgtcagattg cggttcgtgc ggctgcttgt
    22741 agttccagct catcttcatc agcggttcta cgcctttacc accttcagcc tggtacagct
    22801 cgcgcagatg atggtagata cccgccagaa tttcgccgtc gttacgcgct tcgcccggcg
    22861 cgtcctgacc tttccagtgc cactgcaacc agcgaccgga gttagcgata gaaccatctt
    22921 cttctgcaaa gcaggtcgaa ggcagacgga atacttcagt ctgaatagac gccggatcga
    22981 catcgttcga ctcgccgtgg ttctgccaga aggtagaggt ttcagtcacc agcggatcga
    23041 taaccaccat gtacttcagc ttgctcaggc agctcaccac tttgtttttg tccgggaagg
    23101 acgcaaccgg gttaaagccc tggcagaaat aaccggtgac tttgccttca tccatcatgt
    23161 tgaaatactt gatgacgtcg taggtctggt cccacttcgg cagccagtca tagccccagt
    23221 tgttctcttt ctgcgcggca tcgccataga aagatttcat caggctaacg aagaacttcg
    23281 gatagttgct ccagtagttc acctgaccag ccagcgtcgc tttcggcgtg ttcgcttcca
    23341 gatacgactg caaatcaacc tgtttttctg acggcagcgt cagataacct ggcaggctgg
    23401 tagagagcag acctaagtca gtcagaccct gaatgttgga gtgaccacgc aatgcattca
    23461 cgccgccacc ggccataccc atgttaccga gcagcaactg gatcatcgcc atagtacgga
    23521 tgttctgcgc acccacggtg tgctgcgtcc agcccagcgc gtacaggaag gtggttgtgc
    23581 gatccggtgc gctggtggag gccagcactt cacacacttt caggaagtcg gcttttggcg
    23641 taccacagat gttttcaaca acgtccggcg tgtagcggga aacgtgctct ttcagcaggt
    23701 tccacacaca gcgcggatga gtcagtgttt catcgcgttt tgcatagccg ttttcatcga
    23761 actgatagtt ccaggacgac ttatcgtact ggcgtttttc agcgtcgtag ccgctgaaca
    23821 gaccgtcttc gaaagcaaaa tcatcacgca ccagcaggct ggcgttggtg taatgcttaa
    23881 cgtattcggc gttgattttg ttgttttcga tcaggtagcg caaaacgcca gacaggaacg
    23941 taatgtccgt accggaacga ataggcgcgt agatatccgc cacagaagcg gtacgcgtaa
    24001 aacggggatc gacaacgatc aaggttgcat cgttgttgtt tttcgcttcc atcgcccagc
    24061 ggaaaccgac gggatgcgct tcagcagcgt taccgcccat taccattacg acgttagcgt
    24121 ttttgatatc cacccagtgg ttggtcatcg caccgcgacc aaatgttgga gcaagacttg
    24181 ctaccgttgg tccgtgtcag acgcgcgcct ggttgtctac cgccagcatc ccgagggagc
    24241 gggcaaattt ctgggtcagc atcccggttt cgttgctggc accggaagca cacagcatac
    24301 cggtagaaag ccaacggttt accgttacgc cctgctcgtt cttttcaata aagttagcgt
    24361 cacggtcagc tttcatcagc ttcgcaatac gggagaatgc ttcttcccag ctaatgcact
    24421 gccatttgtc agaacctggc gcacgatatt ccgggtagcg cagacggttt tcgctgttga
    24481 cgtaatccag caaaccggcc cctttcgggc acagcgcccc acggcttacc ggatgatccg
    24541 ggtcaccttc aatgtgataa atcgcttctc tggcgttttt cgcgccatca cccaggctat
    24601 acatcaatag cccgcaacct acggaacagt atgtgcaggt gttacggatc tctttagcgc
    24661 gtaataattt gtagtttcgc gcctgagcca gtgcttgctt cggggcaaag cccaatgccg
    24721 ctactgttgt tccagccata ccgcccgcgc agattttaaa aaattgtctg cgactgacgt
    24781 ccattgcttt cctctttttt caggggtatt actgcgcgag gaaactaaca gtaaaattct
    24841 gaatcttttt gcgcgaaatc aaaaagaaca cgtgtttgcc acaaaataat ccgcctgcgc
    24901 aaattgacct acctcaatag cggtagaaaa acgcaccact gcctgacagg ccagttaaaa
    24961 aaatgctata aaattcagct taatttttaa cggcaagaga gacaaaacag cgagcatgac
    25021 acgacaaaaa gcaacgctca tagggctaat agcgatcgtc ctgtggagca cgatggtagg
    25081 attgattcgc ggtgtcagtg aggggctcgg cccggtcggt ggcgcagctg ctatctattc
    25141 attaagcggg ctgctgttaa tcttcacggt tggatttccg cgtattcggc aaatcccgaa
    25201 aggctattta ctcgccggga gtctgttatt cgtcagctat gaaatctgtc tggcgctttc
    25261 cttagggtat gcggcgaccc gtcatcaggc gattgaagtg ggtatggtga actatctgtg
    25321 gcccagcctg acaattctct ttgccattct gtttaatggt cagaaaacca actggctgat
    25381 tgtaccagga ttattattag ccctcgtcgg cgtctgctgg gtgttaggcg gtgacaatgg
    25441 gttacattat gatgaaatca tcaataatat caccaccagc ccattgagtt atttcctggc
    25501 gttcattggt gcgtttatct gggcagccta ctgcacagta acgaataaat acgcacgcgg
    25561 atttaacgga attaccgttt ttgtcctgct aacgggagca agtctgtggg tttactattt
    25621 tctcacgcca caaccagaaa tggtatttag cacgccagtc atgattaaac tcatctctgc
    25681 ggcatttacc ttaggatttg cttatgctgc atggaatgtc ggtatactgc atggcaacgt
    25741 caccataatg gcggtaggtt cgtattttac gcccgtactt tcctcagcgc ttgcagccgt
    25801 gctgctcagc gccccgctgt cgttctcgtt ctggcaaggc gcgctgatgg tctgcggcgg
    25861 ttccctgctc tgctggctgg cgacacgtcg tggctaaaat aattgccggg tctacggccc
    25921 ggcaattaat cagataaacc ccgacaatat taaaaaataa atttcaaata ccagtaattc
    25981 ac
//
LOCUS       DAZFBR010000071        24844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFBR010000071 DAZFBR010000000
VERSION     DAZFBR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.071"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(296..2869)
                     /gene="clpB"
                     /locus_tag="ACHJZ2_003063"
     CDS             complement(296..2869)
                     /gene="clpB"
                     /locus_tag="ACHJZ2_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO4569136.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2999..3730)
                     /gene="yfiH"
                     /locus_tag="ACHJZ2_003064"
     CDS             complement(2999..3730)
                     /gene="yfiH"
                     /locus_tag="ACHJZ2_003064"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO4569137.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3727..4707)
                     /gene="rluD"
                     /locus_tag="ACHJZ2_003065"
     CDS             complement(3727..4707)
                     /gene="rluD"
                     /locus_tag="ACHJZ2_003065"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO4569138.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4842..5579
                     /gene="bamD"
                     /locus_tag="ACHJZ2_003066"
     CDS             4842..5579
                     /gene="bamD"
                     /locus_tag="ACHJZ2_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO4569139.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5850..6191
                     /gene="raiA"
                     /locus_tag="ACHJZ2_003067"
     CDS             5850..6191
                     /gene="raiA"
                     /locus_tag="ACHJZ2_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO4569140.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6294..6419
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6295..6342
                     /gene="pheL"
                     /locus_tag="ACHJZ2_003068"
     CDS             6295..6342
                     /gene="pheL"
                     /locus_tag="ACHJZ2_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO4569141.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6441..7601
                     /gene="pheA"
                     /locus_tag="ACHJZ2_003069"
     CDS             6441..7601
                     /gene="pheA"
                     /locus_tag="ACHJZ2_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO4569142.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7644..8765)
                     /gene="tyrA"
                     /locus_tag="ACHJZ2_003070"
     CDS             complement(7644..8765)
                     /gene="tyrA"
                     /locus_tag="ACHJZ2_003070"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO4569143.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8776..9846)
                     /gene="aroF"
                     /locus_tag="ACHJZ2_003071"
     CDS             complement(8776..9846)
                     /gene="aroF"
                     /locus_tag="ACHJZ2_003071"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO4569144.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10056..10421
                     /gene="yfiL"
                     /locus_tag="ACHJZ2_003072"
     CDS             10056..10421
                     /gene="yfiL"
                     /locus_tag="ACHJZ2_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO4569145.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10571..11089
                     /gene="yfiR"
                     /locus_tag="ACHJZ2_003073"
     CDS             10571..11089
                     /gene="yfiR"
                     /locus_tag="ACHJZ2_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO4569146.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11079..12305
                     /gene="dgcN"
                     /locus_tag="ACHJZ2_003074"
     CDS             11079..12305
                     /gene="dgcN"
                     /locus_tag="ACHJZ2_003074"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO4569147.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12321..12803
                     /gene="yfiB"
                     /locus_tag="ACHJZ2_003075"
     CDS             12321..12803
                     /gene="yfiB"
                     /locus_tag="ACHJZ2_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO4569148.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12880..13227)
                     /gene="rplS"
                     /locus_tag="ACHJZ2_003076"
     CDS             complement(12880..13227)
                     /gene="rplS"
                     /locus_tag="ACHJZ2_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO4569149.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13269..14036)
                     /gene="trmD"
                     /locus_tag="ACHJZ2_003077"
     CDS             complement(13269..14036)
                     /gene="trmD"
                     /locus_tag="ACHJZ2_003077"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO4569150.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14067..14615)
                     /gene="rimM"
                     /locus_tag="ACHJZ2_003078"
     CDS             complement(14067..14615)
                     /gene="rimM"
                     /locus_tag="ACHJZ2_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO4569151.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14634..14882)
                     /gene="rpsP"
                     /locus_tag="ACHJZ2_003079"
     CDS             complement(14634..14882)
                     /gene="rpsP"
                     /locus_tag="ACHJZ2_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO4569152.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15019..16380)
                     /gene="ffh"
                     /locus_tag="ACHJZ2_003080"
     CDS             complement(15019..16380)
                     /gene="ffh"
                     /locus_tag="ACHJZ2_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO4569153.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMTGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16547..17338
                     /gene="ypjD"
                     /locus_tag="ACHJZ2_003081"
     CDS             16547..17338
                     /gene="ypjD"
                     /locus_tag="ACHJZ2_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO4569154.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17360..18646
                     /gene="yfjD"
                     /locus_tag="ACHJZ2_003082"
     CDS             17360..18646
                     /gene="yfjD"
                     /locus_tag="ACHJZ2_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO4569155.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVVGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18701..19294)
                     /gene="grpE"
                     /locus_tag="ACHJZ2_003083"
     CDS             complement(18701..19294)
                     /gene="grpE"
                     /locus_tag="ACHJZ2_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO4569156.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19417..20295
                     /gene="nadK"
                     /locus_tag="ACHJZ2_003084"
     CDS             19417..20295
                     /gene="nadK"
                     /locus_tag="ACHJZ2_003084"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO4569157.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20381..22042
                     /gene="recN"
                     /locus_tag="ACHJZ2_003085"
     CDS             20381..22042
                     /gene="recN"
                     /locus_tag="ACHJZ2_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO4569158.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22191..22532
                     /gene="bamE"
                     /locus_tag="ACHJZ2_003086"
     CDS             22191..22532
                     /gene="bamE"
                     /locus_tag="ACHJZ2_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO4569159.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22594..22884)
                     /gene="yfjF"
                     /locus_tag="ACHJZ2_003087"
     CDS             complement(22594..22884)
                     /gene="yfjF"
                     /locus_tag="ACHJZ2_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO4569160.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22874..23311)
                     /gene="ratA"
                     /locus_tag="ACHJZ2_003088"
     CDS             complement(22874..23311)
                     /gene="ratA"
                     /locus_tag="ACHJZ2_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO4569161.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23482..23964
                     /gene="smpB"
                     /locus_tag="ACHJZ2_003089"
     CDS             23482..23964
                     /gene="smpB"
                     /locus_tag="ACHJZ2_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO4569162.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24179..24541
                     /gene="ssrA"
                     /locus_tag="ACHJZ2_003090"
     tmRNA           24179..24541
                     /gene="ssrA"
                     /locus_tag="ACHJZ2_003090"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         6319 a         6547 c         5831 g         6147 t
ORIGIN      
        1 ccgcttcgct ttttctcagc ggcgcggggt gtgcatgata cgctttcccg ctacagagtc
       61 aagcatttat ttttgctttc tctgccggaa ttctcaggag aaccccgctg acccggcggc
      121 gtgtttgccg ttgttccgtg tcagtggtgg cgcattatag ggagttattc cggcctgaca
      181 agcgaaaaat ataaaaactt tatcgttcgc tcatttttca ggcaaaacat cttaaatata
      241 gtcttttccg tctaacttat agacaaaaac gagccccgaa gggctcgttt tatcattact
      301 ggacggcgac aatccggtct tcattaactt ccaggcgaat aactttaccc ggaaccaatt
      361 caccagacag tatttgctgt gccaacgggt tttcgatctg ctgctggata gcacgtttca
      421 aaggacgtgc accatagact ggatcgtaac cgttctcgct cagcagtttc agcgcctcgt
      481 cagaaatgtg gatttcataa ccacgttctt ccagacgttt gtacagacgt ttcaactgaa
      541 tctgcgcaat cgaggcaatg tgctgttcgc ccagcggatg gaagaccacc acttcatcga
      601 tacggttaat gaactccgga cggaagttat ggcttaccac accgagcacc agctctttca
      661 tgtgcgcata atccagttca ccgaagcgtt cctgaatcag atcggaaccg aggttagagg
      721 tcataatgac gaccgtatta cggaagtcga ccgttctccc ttgcccgtca gtcagacgcc
      781 catcatccag tacctgcaac agaatgttga agacatccgg atgcgctttt tccacttcat
      841 ccagcaggat gacggaatac ggacgacgac gcaccgcttc ggtcaggtag ccaccttctt
      901 cataaccgac atatcccgga ggcgcaccaa ccagacgaga caccgagtgt ttctccataa
      961 actcggacat atcgatacgg accatcgcct cgtcgctatc aaacataaag ttcgccagcg
     1021 ccttacaaag ctctgttttc cccacaccag ttgggccgag gaacaggaat gaaccaatcg
     1081 ggcgatttgg atccgccagc cccgcacggc tacgacgaat agcgttagat accgcatcaa
     1141 ccgcttcgtt ctgaccaatt acgcgatggt gcagttcttg ctccatacgc agcagttttt
     1201 cgcgctcgct ttccatcatg cgagaaaccg gaatccccgt ccaacgcgcc agcacttcag
     1261 caatttcggc gtcggtcact ttattacgca acagacgcat agttttgcct tcgagctgcg
     1321 ttgcggcttc cagttgcttt tccagttccg ggattttgcc gtattgcagt tcagacatcc
     1381 gcgccaagtc acccacacgg cgagcctgtt caatagcgat tttcgcctgt tccagttccg
     1441 ctttaatggt ctgcgtacca gaaagcgatg ccttctctgc tttccactct tcttctaact
     1501 cggagtactg acgttctttg tcgctcagtt cttcgttgag catatccaga cgttttttac
     1561 tggcttcatc agactctttc attaacgcct gttgttccag tttgagctgg atgatacggc
     1621 gatcgagtcg gtcgagttct tctggttttg agtcaatctg catacgaatg ctggatgctg
     1681 cttcatcgat caggtcgatg gctttatccg gcagctgacg gtcagcaatg tagcgatgag
     1741 acaacgtcgc cgctgcaaca attgccgggt cagtaatttg cacatggtgg tgcaattcgt
     1801 aacgttcttt caggccacgc agaatcgcaa tggtatcttc aacagaaggc tcggcaacaa
     1861 acactttctg gaaacgacgt tccagcgcag catctttttc gatgtactgg cgatattcgt
     1921 caagcgtcgt ggcacctacg cagtgcaatt caccacgcgc cagcgccggt ttcagcatgt
     1981 ttccggcgtc cattgcgcca tcggctttac ccgcgccgac catggtatgt aattcgtcga
     2041 taaacaggat gacgttgcct tcctgtttgg caagatcgtt aagcacgcct tttaaacgtt
     2101 cttcaaactc accgcgatat ttcgccccag ccaccagcgc gcccatatcc agcgccagta
     2161 cccggcggcc tttcaaccct tccggcactt cgccgttgat aatacgctgc gccagacctt
     2221 caacgatggc agttttaccg acgccgggtt caccaatcag taccgggtta tttttagtac
     2281 gacgttgcag cacctgaatg gtacggcgaa tttcttcatc acgaccaatc accggatcga
     2341 gtttgccctg ttcggctcgt tcggtaaggt cgatggtata ttttttcaaa gcctgacgtt
     2401 ggtcttcagc accttgatcg ttcacgcttt cacctccacg catttgttca atcgcttgag
     2461 taatgttggc ggtggtcgcc cctgctgctt tcaggatgtc ggccagcgtg ccgcgagact
     2521 caagtgccgc cagaacgaac agttctgacg agataaagtt atcaccacgt ttttgcgcca
     2581 gcttgtcgca aagattaaga acgcgcacca gatcctgtga tggctggaca tcaccaccag
     2641 taccttcaac ctgcggtaaa cgatttaatg cctgattgat atctgtgcgc aactggccag
     2701 catttatgcc agcggatgtt aataaaggac taaccgaacc cccttcctga ttcagcaggg
     2761 cgctcattaa atgaagtggt tcgataaatt ggttgtcgtg cccaagtgca agtgattggg
     2821 catcggcaag agcaagctgg aatttattag taagacgatc cagacgcata actcctccca
     2881 taacggatca aagttgctac tggagattaa atgaggtcat ccctcaatta ttcaaggtta
     2941 ttgaccagat taatgtgaaa agaaaatcac gcgtaccgga tcgtcttgat tctttaggtt
     3001 atatcagcca aatgaaactt gccatacgac cggtggtctt gtcgcgacga taagagaaga
     3061 aagtctcatt ttccgtatat gtacaacggt cgccgccgaa aatttgctca acacccacgt
     3121 tcgccagacg ctgccgggca agctgataaa tgtccgccag atacttatca ccatgctgaa
     3181 tgaaagctgt actcgcttcg gcgtctactg ccataaacgc ctcgcgaacc tccgccccca
     3241 cttcaaacgc gcgtgggcca attgccggcc ctaaccaggc gagaatattt tctggattat
     3301 cagcaaaaca ggaaactgtc tcttccagca cgcctgcgca cagtccacgc cagccagcat
     3361 gagcggcggc gacttccgtt cccgctcgat tgcaaaacag cacagggagg cagtcggcag
     3421 tcatcactgc gcaaaccctg ccgggcgtat tgctataaga ggcatctgct cgttttgagg
     3481 cataaggttc gccagtaaga ttaagcacat ctttgccgtg tacctgctca agccagaccg
     3541 gtttagaagg caaattgccc gcagcaaaaa gtcgcttgcg attctcctca acgtgatccg
     3601 ggttatcgcc acaatgggca ccgaggttga gcgagtcata cgggggcaag ctcacgccgc
     3661 cgatacgagt agagctacag gccgcaacac cttttggcag cggccactgc gggacaatca
     3721 gcttactcat aaccagtcca cttcatcttt atgctcttcg aaatcggcgc gcatcacctc
     3781 aatcagctcc accatatctt gtggaatagg cgcatgccat tccatttcga taccggagat
     3841 cgggtgataa agacgcagca tggttgcatg aagcgcctgg cggtcaaatt tacgcagcgt
     3901 ggagataaac gcttccgaag caccttttgg cgggcgcgga cggccaccat aaaccggatc
     3961 gcccaccagc ggatgagtga tatgggccat atgcacgcgg atctggtgcg tacgtccagt
     4021 ttccagacgc aaccgcagac gcgtgtgcac acggaagtgt tccatgatgc gatagtgagt
     4081 caccgccggt ttgcccatcg gatgcaccgc catgtgagta cgtttggtcg ggtggcggct
     4141 gattggttca tctactgtac cgccagcggt catatgacca attgccaccg cttcatactc
     4201 acgagtaatt tcacgccttt gcaaagattc gactaaacgc gtctgagccg gaacggtttt
     4261 tgccacaacc atcaggccag tggtgtcttt atccagacga tggacgatgc ctgcacgcgg
     4321 tacatcagca atgggcggat agtaatgaag taacgcattc agtaccgtgc catccgggtt
     4381 acccgcgcca ggatgtacca ccagatcgcg cggtttatta atgacgatga tatcttcatc
     4441 ttcatagacg atatccagcg ggatatcctg cggttcaaaa cgcgcttctt cttcaatctc
     4501 agcgttgatg gcaacctgct cgccacccaa tactttttct ttcggcttat cacaaagttt
     4561 gccgttaacc agcacgcgct ggtcgaggat ccattctttt attcgcgaac gtgaataatc
     4621 cgggaacatt tcggccaaag cctgatctaa gcgttgaccg agttggtttt cggacaccgt
     4681 tgcagtgagc tgtactcgtt gtgccatata tactgcttct tcgtttatcg ttggggtttt
     4741 acggctttgc cgtttaatat agtgtgctat tgtagctggt cttaaccggg agcaggaaca
     4801 gagaatctcc cgtattacat tttgaggaaa gtcaaaacgt catgacgcgc atgaaatatc
     4861 tggtggcagc cgccacacta agcctgtttt tggcgggttg ctcggggtca aaggaagaag
     4921 tacctgataa tccgccaaat gaaatttacg cgactgcaca acaaaagctg caggacggta
     4981 actggagaca ggcaataacg caactggaag cgttagataa tcgctatccg tttggtccgt
     5041 attcgcagca ggtgcagctg gatctcatct acgcctacta taaaaacgcc gatttgccgt
     5101 tagcgcaggc tgccatcgat cgttttattc gccttaaccc gacccatccg aatatcgatt
     5161 atgtcatgta catgcgtggc ctgaccaata tggcgctgga tgacagtgcg ctgcaagggt
     5221 tctttggcgt tgaccgtagc gatcgcgatc ctcaacatgc acgagctgcg tttagtgact
     5281 tttccaaact ggtgcgcggc tatccaaaca gtcagtacac caccgatgcc accaaacgtc
     5341 tggtattcct gaaagatcgt ctggcgaaat atgaatactc cgtggccgag tactatacag
     5401 aacgtggcgc atgggttgcc gtcgttaacc gcgtagaagg catgttgcgc gactacccgg
     5461 atacccaggc tacgcgtgat gcgctgccgc tgatggaaaa tgcataccgt cagatgcaga
     5521 tgaatgcgca agctgaaaaa gtagcgaaaa tcatcgccgc aaacagcagc aatacataac
     5581 agaaacctga aacacaaaac ggcagccctt gagctgccgt ttttttattc tgtcagttgt
     5641 gaaactgaag cgatttagtc actatcgatc tcatcaaata tggctcgctt tgagatattc
     5701 ctcaagtaaa aaaacacctc ttcctgcgat ttctcacaaa aaagattcgt tgacaaaaag
     5761 tgacaaaatt atgagatttc catcacacat tttgacatca ggaacggtat gctgaattca
     5821 ccaagacggg aagacaagag gtaaaattta tgacaatgaa cattaccagc aaacaaatgg
     5881 aaattactcc ggccatccgc caacatgtcg cagaccgtct cgccaaactg gaaaaatggc
     5941 aaacacatct gattaatcca catatcattc tgtccaaaga gccacaaggg tttgttgctg
     6001 acgccacaat caatacacct aacggcgttc tggttgccag tggtaaacat gaagatatgt
     6061 acaccgcaat taacgaattg atcaacaagc tggaacggca gctcaataaa ctgcagcaca
     6121 aaggcgaagc acgtcgtgcc gcaacatcgg tgaaagacgc caacttcgtc gaagaagttg
     6181 aagaagagta gtcctttata ttgagtgtat cgccaacgcg ccttcgggcg cgttttttgt
     6241 tgacagcgtg aaaacagtac gggtactgta ctaaagtcac ttaaggaaac aaacatgaaa
     6301 cacacaccgt ttttcttcgc attctttttt accttcccct gaatgggagg cgtttcgtcg
     6361 tgtgaaacag aatgcgaaga agaacaataa ggcctcccaa atcggggggc cttttttatt
     6421 gataacaaaa aggcaacact atgacatcgg aaaacccgtt actggcgctg cgagagaaaa
     6481 tcagcgcgct ggatgaaaaa ttattagcat tactggcaga gcggcgcgaa ctggccgtcg
     6541 aggtgggaaa agccaaactg ctctcgcatc gcccggtacg tgatattgat cgtgaacgcg
     6601 atttactgga aagattaatt acgctcggta aagcgcacca tctggacgcc cattacatta
     6661 ctcgcctgtt ccagctcatc attgaagatt ccgtattaac tcagcaggct ttgctccaac
     6721 aacatctcaa taaaattaat ccgcactcag cacgcatcgc ttttctcggc cccaaaggtt
     6781 cttattccca tcttgcggcg cgccagtatg ctgcccgtca ctttgagcaa ttcattgaaa
     6841 gtggctgcgc caaatttgcc gatattttta atcaggtgga aaccggccag gccgactatg
     6901 ccgtcgtacc gattgaaaat accagctccg gtgccataaa tgacgtttac gatctgctgc
     6961 aacataccag cttgtcgatt gttggcgaga tgacgttaac tatcgaccat tgtttgttag
     7021 tctccggcac gactgattta tccaccatca atacggtcta cagccatccg cagccattcc
     7081 agcaatgcag caaattcctt aatcgttatc cgcactggaa gattgaatat accgaaagta
     7141 cgtctgcggc aatggaaaag gttgcacagg caaaatcacc gcatgttgct gcgttaggaa
     7201 gcgaagctgg cggcactttg tacggtttgc aggtactgga gcggattgaa gcgaatcagc
     7261 gacaaaactt cacccgattt gtggtgttgg cgcgtaaagc cattaacgtg tctgatcagg
     7321 ttccggcgaa aacgacgttg ttaatggcga ccgggcaaca agccggtgcg ctggttgaag
     7381 cgttgctggt actgcgcaac cacaatctga ttatgacccg tctggaatca cgcccgattc
     7441 acggtaatcc atgggaagag atgttttatc tggatattca ggccaatctt gaatcagcgg
     7501 aaatgcaaaa agcattgaaa gagttagggg aaatcacccg ttcaatgaag gtattgggct
     7561 gttaccctag tgagaacgta gtgcctgttg atccaacctg atgaaaaggt gccggatgat
     7621 gtgaatcatc cggcactgga ttattactgg cgattgtcat tcgcctgacg caataacacg
     7681 cggctttcac tctgaaaacg ctgtgcgtaa tcgccgaacc agtgctccac cttgcggaaa
     7741 ctgtcaataa acgcctgctt atcgccctgc tccagcaact caatcgcctc gccgaaacgc
     7801 ttatagtaac gtttgattaa cgccagatta cgctctgacg acataataat gtcggcataa
     7861 agctgcggat cctgagcgaa cagtcgcccg accatcgcca gctcaaggcg gtaaatcggc
     7921 gaagagagcg ccagaagttg ctcaagctga acattttctt ctgccaggtg cagcccgtaa
     7981 gcaaaagtag caaagtggcg cagtgcctga ataaacgcca tattctgatc gtgctcgacg
     8041 gcgctaatac gatgcaaccg agcgccccag acctgaattt gctccagaaa ccattggtat
     8101 gcttccggtt tacgtccatc acaccagacc acaacttgct ttgccaggct accgctgtcc
     8161 ggaccgaaca ttgggtgtaa ccccagtacc gggccatcgt gcgccgccag catggcctgt
     8221 aatggtccat ttttcactga tgccagatca accagaatac aatctttcgg taaaggcggt
     8281 aatttgccaa taacttgctc agtaacgtgg attggcacac taacaatcac cattccggca
     8341 tcggcaacaa tatcagccgc tcgatcccag tcatgttgct ccagaatccg cacctgataa
     8401 cccgagagtg tcagcatctt ctcgaacaga cgtcccatct gaccgccgcc gccaacgata
     8461 actaccgggc gtaacgcagg acaaagcgtt ttaaatcctt tgtcgttttc actggagtaa
     8521 gattcacgca tcacccgacg caaaacatcc tcaatcagat ctggcggtac acccagagct
     8581 tccgcctctg cacgacgcga ggccaacata gatgcctctc gctccggaac ataaataggc
     8641 agtccaaagc ggcttttcac ctcgcccact tcagcaacca gttccagacg cttcgctaat
     8701 aaattcagca gcgctttatc gacttcatca atttgatcgc gtaatgcggt caattcagca
     8761 accataataa acctcttaag ccacgcgagc cgtcagctgc ccgttcagat cctgatgaat
     8821 ttcacgcagc aaggcatcgg tcatttccca gctaatgcag gcatcggtta cggatacacc
     8881 gtatttcatt tcactgcgcg gttgctcgga agactgattg ccctcgtgga tattactttc
     8941 gatcatcaga ccaataattg agcgattgcc atctttgatt tgagcaacca cggattctgc
     9001 caccgcaggc tgacggcgat aatctttatt ggaattaccg tggctgcaat ctaccatcag
     9061 agacgggcgc agtcccgcct gttccatctc tttttcacat tgcgcaacat ccgcagggct
     9121 atagttcggc gctttaccac cgcgcaggat gacatgcccg tccggattcc cctgagtttg
     9181 tagcaacgca acctgccctg cctggttaat gccaacaaaa cggtgcggct gggcggcggc
     9241 gcgcatagcg ttaattgctg ttgccagact gccgtcggtg ccgtttttaa agccaaccgg
     9301 catggaaagc ccggaggcca tttcacggtg agtttgcgat tccgttgtac gagcaccaat
     9361 tgctgaccag ctaaacagat cgcccaggta ttgcgggcta ttcggatcta acgcttccgt
     9421 cgccagtggc agtcccatat tcaccagctc aagcagcaat ttacgcgcga tctgcagccc
     9481 ggcttctaca tcaaaagagc catccatatg gggatcgtta attaaccctt tccagccgac
     9541 agtggtacgg ggtttttcaa aatagacgcg cattaccaga tagaggctat cgctgacctc
     9601 tgcggcaagg gctttaaatc gacgagcata ttccagagca gtttccggat catgaatgga
     9661 acaagggcca catactacca gcagacgagg atcgcgcccg gcgataatat ctgaaatggt
     9721 tttacgcgag tcagcaatct gggcttcttg ttgcaggctt aatggaaaag cggccttcag
     9781 ttgtgccgga gtcattaaaa cctgttcgtc ggtaatatgt acgttattca gcgcgtcttt
     9841 ttgcatgatg gcgatcctgt ttatgctcgt ttgcgatagt tgatcctcag cgaggatgac
     9901 gtaacgataa cacataaagt aaagttttca atccatattt cgtacatttt tctttacata
     9961 agcaatttat tcgcgctttc aacccttgat tctgtataga taaatttaca ccacctttga
    10021 aaacaatccg ctatgctttg aaaaaggaga aagaaatgat gaaaaagttt atcgccccct
    10081 tgttggcttt actggttagc ggatgccaga ttgatcctta tactcacgcg ccaaccttga
    10141 ccagcaccga ctggtatgat gttggtatgg aagatgcgat atcgggcagc gccataaaag
    10201 atgacgatgc atttagcgat tcacaggcgg atcgcggtct ataccttaaa ggatatgccg
    10261 aaggacaaaa gaaaacttgc cagaccgatt ttacttatgc ccgaggactt tccggtaaaa
    10321 gctttcctgc cagctgcaac aatgttgaaa atgccagcca actgcatgaa gtatggcaaa
    10381 aaggggctga tgaaaatgcc agcgcaatac gattaaatta attaaaaccc cacaaataaa
    10441 ttaaacataa gattttactc attatgtttt atttctcaca atgatgacga ccgcatgaaa
    10501 taatgatatt ttaatgagca ttctgaaaaa atatcgatgc atttcgagcg aagatggtgg
    10561 ggatccctga atgcgttttt ctcaccgact gttcttactg ctgattctcc tgctgacggg
    10621 agcaccaata ctcgctcagg agccttctga cgttgcgaaa aacgtgcgta tgatggtttc
    10681 cggtattgtc agttacaccc gctggcctgc gctatcaggg ccaccaaagt tatgtatctt
    10741 ttcatcttca cgttttagta ccgccctaca agagaatgcc gcaacatctt tgccttatct
    10801 gcctgtcatc attcacactc aacaagaggc gatgatttca ggctgcaatg gtttttattt
    10861 tggcaacgag tcaccgacat tccagatgga attaaccgaa caatatccgt caaaggcgtt
    10921 attattaatt gccgaacaaa ataccgagtg cattattggt agcgcctttt gtctgatcat
    10981 ccacaataat gacgtcagat ttgccgtaaa cctggatgcc ttatcgcgta gcggtgtaaa
    11041 ggtcaacccg gatgtcttaa tgctcgcacg gaagaaaaat gatggataac gataattctc
    11101 ttaataagcg ccccacgttt aaaagagcat tacgcaacat cagtatgacc agcatattta
    11161 tcactatgat gctgatctgg ttgctgcttt ccgtgacctc ggtgctgccc ctgaaacagt
    11221 acgcgcaaaa aaacctggca ctgacagcag caacaatgac ttacagtctg gaagcagctg
    11281 tcgtttttgc cgatggccct gcagcaactg aaacactggc agcgctgggc cagcaagggc
    11341 aattttcaac tgcagaagta cgtgataagc agcaaaatat tctggcatcc tggcattaca
    11401 cccgtaagga tccaggcgat actttcagca atttcataag ccactggctc ttccctgccc
    11461 ccatcattca gccgattcgt cacaatggtg aaaccattgg cgaagtacgc ttaaccgctc
    11521 gcgacagttc aatcagccat tttatctggt tttcgctcgc cgtactgacc ggttgtattc
    11581 tgctggcatc aggaatcgca attaccctca cccgccattt gcacaatggc ctggtggaag
    11641 cactgaaaaa tatcaccgat gtcgtacatg atgtgcgttc caaccgcaat ttttcccgac
    11701 gagtttcgga agaacgtatc gctgagtttc accgcttcgc tctcgacttc aacagtctgc
    11761 tggatgaaat ggaagagtgg cagcttcgtt tacaggccaa aaatgcgcag cttctacgta
    11821 ccgcgctaca tgacccatta accgggctgg ctaaccgcgc agcgtttcgt agcggcatca
    11881 atacgttgat gaacaattcc gctgcccgaa aaacgtcggc gttactattt cttgatggcg
    11941 ataatttcaa atatatcaat gatacctggg gtcatgcgac gggcgataga gtcttgattg
    12001 aaatcgcaaa acggttagct gaatttggcg ggctgcgaca taaagcatac cgcctgggcg
    12061 gcgatgaatt cgctatggtg ctctatggtg tacagtcgga atctgaagtg cagcagatat
    12121 gctcagcact gacacaaatc tttaatctcc cgtttgatct tcataatggc catcagacca
    12181 ccatgacatt aagcattggt tacgcaatga ccattgcgca cgcttctgcg gaaaaattac
    12241 aagagctggc cgatcacaat atgtatcagg ccaaacacca gcgtgccgaa aagctggtga
    12301 gataacaagg atatatcgat atgataaagc acctggtagc acccctgatt ttcacctcac
    12361 taatactgac tggctgccag tcccctcagg gaaagtttac tcctgagcaa gtcgccgcta
    12421 tgcaatctta tggatttact gaatccgccg gcgactggtc gctgggctta tcagatgcca
    12481 ttctgttcgc aaaaaatgac tacaaattgc tcccggaaag ccagcaacag atccaaacca
    12541 tggcagccaa attggcctcg acagggctaa cacatgcccg tatggatgga cacaccgata
    12601 actatggtga agacagttac aacgaaggct tatcattgaa acgggcgaat gtcgtggccg
    12661 atgcatgggc tatgggtgga caaattccac gcagcaatct caccacacag ggtttaggaa
    12721 aaaaatatcc catagccagt aacaagaccg cccagggccg cgccgagaac cgccgcgtcg
    12781 cagtggtgat tactacccct taaaacacga gcaaacagat aaaaaaaggg ccagccaatt
    12841 ggccagccct tcttaacagg atgtcgctta agcgaaatct tagttaagac gctctttgat
    12901 acgagcagcc ttaccagtac gctcacgcag gtagtacagt ttagctttac gaacagcacc
    12961 acgacgtttg acagaaatgc tgtcaactac cggagagtga gtctggaaga cacgctcaac
    13021 gccttcgccg ttggaaattt tacgaacagt gaatgcagag tgcagaccgc ggttacgaat
    13081 agcgataacc acgccctcga atgcctgcag acgttttttg gaaccttcaa caacccatac
    13141 tttcacttcc acggtatcac ccggacggaa ggaaggtacg tcctgcttca tctgctcttg
    13201 ttcaagttgc ttaataatgt tgctcataat ttaatctctt atcctgggta aactgatatc
    13261 tcgggggctt acgccatccc atcatgttta tgttgctgtt gtgcgtgttc cgttttgaac
    13321 tccgccagca accttgcttg ctcttcagtc agagccaggt tttccagaag ttcaggtctt
    13381 ctaagccagg tacggcccag cgactgtttc aaacgccagc gacgtatctc ggcatggttg
    13441 cccgacagta acactggcgg aacttccatc ccttctaaca cctcaggccg cgtatagtgc
    13501 gggcaatcca gcaatccttc agcaaaggaa tcttccgttg ccgaggcttc atgtcccagt
    13561 actcccggaa taaaccggga aacggagtca atcagcgtca ttgctggtaa ctcaccacca
    13621 ctgagaacgt aatcgccgat tgaccattct tcgtcaattt cggtttggat cacgcgctca
    13681 tctatacctt cgtagcgacc gcacaccaga atcaattttt gattcgttgc cagttcgctg
    13741 acgcccgctt gatcaagctt gcgtccctgt ggtgaaagat aaatcacctt tgcgccttca
    13801 cccgccgcgg cttttgctgc atgaatggcg tcccgcaagg gttgcaccat cattaacatc
    13861 cccggtccgc cgccgtaagg acgatcgtcc acggtacggt gccggtcatg cgtgaaatcg
    13921 cgaggactcc agctctggat gctcagcagg ccatttttaa ctgcccggcc agttaccccg
    13981 taatcggtaa ttgcgcggaa catttcagga aacaggctaa ttatgccaat ccacatagcg
    14041 ccgtctttta ccgtttatcc ggtggtttaa aaaccaggat cccaatctac ttcgattgaa
    14101 cgagtagtga gatcgacttt cttgataacc tgcccatcga ggaacggtac gagacgttcc
    14161 ttgataccaa acgcatcttt caggtttgcc ttaatgacga gaacgtcatt ggatccggtt
    14221 tccatcatat cgacgacttt accgagatcg tagccttcag tggttactac ctggcagccc
    14281 atcaggtctt tccagtagta gtcaccctct tcaagctgag gcagctgcga tgaatccacg
    14341 acaatttcac aattcgtcag caggttcgcc gcatcacgat cgtcaacgcc tttcagcttg
    14401 atgatcatgt cctgattgtg gtgcttccag ctttccagct ggacttgctg ccactgaccc
    14461 gccttctgga taaaccaggg ctgatagtca aaaatgcttt cggcgtcttc ggtggaagaa
    14521 aacactctga gccacccacg aataccgtaa gacgaaccca tttttcccaa aacgatggga
    14581 tcaacaggtg cttgcgcggt gagttgtttg ctcatcatga ccaccgtgac agattaagct
    14641 gctttgttta cttctttgat cagcgcagca acgcgatcag aaatagttgc gccctggcca
    14701 acccagtgag cgatgcgatc cagatccagg cgagtgcctt cttctttttc gctagcgatt
    14761 gggttgaaga aaccaacgcg ctcgatgaag cgaccgttgc gtgcattacg gctgtcagcg
    14821 acaacaacct ggtagaacgg acgcttttta gcgccgtgac gtgctaaacg aatagttacc
    14881 ataacatcct cttgtgtgaa taaaacaacc ggaccccatc gaggaacgga gtccggtgtc
    14941 atattaaaag cccgaaaatt ttactgattt ctggagaaaa tgcaatcagc agttaataac
    15001 tctgtcgcat aaggtcattt agcgaccagg aaatcccggc ggcatcatcc ccttcatgct
    15061 tctcatcatc ttcgccattc cgcccttctt cattttcttc atcatgcgct gcatgtcgtc
    15121 gaactgtttc agaagacggt taacgtcctg cacctgcata ccgcaaccgg cagcaatacg
    15181 gcgtttacgc gaacctttga tgatttctgg cttagcgcgc tctttcatcg tcatcgagtt
    15241 gatgatggct tccatacgca ccagcacttt atcgtccatc tgtgacttga cgttatccgg
    15301 gatctgcccc atgcccggca gtttgcccat caggctggcc atgccgccca tatttttcat
    15361 ctggcgcagt tgctcaagga agtcgttgag atcgaagccg tcaccttttt tcagcttgct
    15421 ggctaatttc tctgcctgcg cgcggtcaac tttgctttcg atatcttcga tcagcgacag
    15481 tacgtcgccc atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg gctccagcgc
    15541 ctcagttttc tcgccaacac cgaggaactt gatcggtttg ccagtgatgt gacgaataga
    15601 gagcgccgca ccgccgcggg catcgccgtc cactttggtc aacactacgc cggtaagcgg
    15661 taacgcttca ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca tggcgtcaac
    15721 cacaaacagg gtttcaaccg ggttaatcga cgcatggact tgtttgatct cgtccatcat
    15781 cgcttcgtca acgtgcagac gaccagcggt atccaccagc agcacgtcgt agaatttcag
    15841 tttggcttct ttcagcgccg cgttaacaat atcaaccggc ttctgaccaa catcagaagg
    15901 gaagaaatca acgcccacct gctctgccag cgtctcaagc tgtttgattg ccgccgggcg
    15961 atagacgtca gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca ggaacttacc
    16021 gagtttacca acgctggttg ttttaccggc accttgcagg cctgtcatca gcacgaccgc
    16081 aggcggttgc gcagccagat tcagggtctg gttctcttcg cccatcgccg caaccagttc
    16141 gttacggacg attttgacga actcctgccc cggcgtcagg ctcttattaa cttcatgacc
    16201 aaccgctttc tcttttacgc gattgataaa ctcacgcact accggcagag cgacgtccgc
    16261 ctccagcagc gccatgcgca cttcgcgcag cgtatctttt acgttgtctt cagtgaggcg
    16321 tccacggcca ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat tatcaaacat
    16381 tgtctctcgc ctggggtgga aacggttggc cgcaatcgcg acacatcatc agtattttgc
    16441 cgcagtataa catgaaggcg tctttgttgt tatgcaacgg ttggagcagc gttcacctga
    16501 cgctatactg cttctctttc ttattgctca aactgtcgac atcactatgc ccgtttttgc
    16561 tctgctcgcg cttgtcgcct actccgtcag tcttgcgctg attgttcccg gtctgctgca
    16621 aaaaaacggc ggctggcggc gcatggctat tatttctgcg gtcattgcgc tggtctgcca
    16681 cgcaatcgct ctggaagccc gcatcctgcc cgacggtgat agcggacaaa acctcagcct
    16741 gctgaacgtt ggttcattgg tcagtttgat gatctgtacg gtaatgacca ttgtggcttc
    16801 tcgcaatcgt ggctggttgc tgctacccat tgtctatgcc tttgcgctta tcaacctggc
    16861 gctggcaacc ttcatgccca atgaatatat cacccatctg gaagctacgc ctgggatgct
    16921 ggtgcacatt ggcttatcgc tcttttccta tgccacgcta attatcgccg ccctgtacgc
    16981 gctgcaactg gcgtggattg attaccaact gaagaacaag aagctggcgt ttaaccagga
    17041 aatgccgcca ttgatgagta tcgagcgtaa aatgttccac atcacgcaga ttggcgtggt
    17101 gctgctaacg ctcacgcttt gcactggcct gttctacatg cacaacttat ttagcatgga
    17161 aaatatcgac aaggctgtgc tctctatcgt ggcgtggttt gtctatattg tgctgctatg
    17221 gggacattat catgaaggat ggcgtggacg tcgcgtcgtc tggtttaacg ttgcgggtgc
    17281 agtcattctg acactggcct acttcggcag ccgaattgtc cagcagttaa tcagctaaac
    17341 ccagaaaagg agtttccccc tggaacacat ttctactact acgttgatca ttattctgat
    17401 catcatggtg gtcatttcag cctatttttc cgggtccgaa accggaatga tgaccctcaa
    17461 ccgctatcgt ctgcgacata tggcgaaaca gggtaatcgc tcggccaaac gcgtcgaaaa
    17521 attgctgcgt aagccagacc gcctgataag cctggtgtta atcggcaata acctggtcaa
    17581 tattcttgcc tccgcgctcg gcactattgt tgggatgcgt ttgtacggcg atgcgggcgt
    17641 ggcaattgcg actggtgtgc tgacttttgt cgtactggtt tttgctgagg tattgccgaa
    17701 aaccattgcc gcgctgtacc cggaaaaagt cgcttatccg agtagttttc tgctggctcc
    17761 cctgcaaatt ttgatgatgc cgctggtctg gttgctgaat gctatcaccc gtatgctgat
    17821 gcgcatgatg ggtatcaaaa ccgatatcgt ggttagcggc tctttgagca aagaagagtt
    17881 gcgcactatc gtgcacgaat cgcgctcaca aatttcccgt cgcaatcagg atatgctgct
    17941 gtcggtgctc gatctggaaa aaatgaccgt tgatgacatc atggtgccgc gcagtgaaat
    18001 tatcggtatt gatatcaacg atgactggaa atctattctg cgccaactct cccactcacc
    18061 tcacgggcgc atcgtgctct accgtgattc gctggacgac gccatcagta tgctgcgagt
    18121 acgtgaagct tggcggttga tgtcggagaa aaaagagttc accaaagaaa ccatgctgcg
    18181 cgccgcggac gagatctatt ttgtgccgga aggtacgccg ctcagcacgc agttggtgaa
    18241 gtttcagcgc aacaaaaaga aagtcggcct ggtcgtcaac gagtatggag acattcaggg
    18301 gctggtgacg gttgaagata ttctggaaga gattgtcggc gatttcacta cgtcgatgtc
    18361 gccaacactt gccgaagaag tcacgccgca aaacgacggt tcggtgatta ttgatggcac
    18421 tgccaacgtg cgggaaatca acaaagcctt taactggcat ctaccggaag atgatgcccg
    18481 cacggttaat ggcgtcattc ttgaggcact ggaagagatc cctgtcgtag gcacccgcgt
    18541 gcgtattggc gagtacgata tcgatattct cgacgtacag gacaatatga ttaagcaggt
    18601 aaaagttttt cctgtgaaac cgctgcgcga gagtgtggcg gagtaacgaa aacggcccgg
    18661 cattcgcatg cagggccgtg aattattacg aaaacagaaa ttacgctttt gctttcgcta
    18721 cagttaccat cgccgcacga atcgtacgac cattcagcgt ataacccttc tgcataatgc
    18781 ccagtacgtt acctggcgca acgtcatcag attccaccat tgcgatagcc tgatgcacat
    18841 tcgggtccag cggcacgtta gtttcggcga tcacttcaac accaaactta cgcacaacat
    18901 ccagcatcga cttcagcgtc agctcaatgc cttcaaccat cgcagacata tccgggttag
    18961 ctttatcagc cacttccagc gcacgatcca ggctatcaat caccggcagc aattcgttga
    19021 tgaatttctc cagcgcgaat ttgtgggctt tttcaatatc cagttcagta cgacgacgca
    19081 ggttttccat ttcggctttt acacgcaaaa tgccgtcacg ttcacgggtc tgggcttcag
    19141 ccagctgagc ttcgagattc gcaatttttt catcgcgcgg atccacctgc tcagcagaag
    19201 cttctggctc aactgcctca atctcttcgt gctgatccat gataatttct tccggggctt
    19261 gcccctcagg cgttttctgt tctttactac tcatgaattt ctccgcgttt ttttcgcatt
    19321 catctcgcta acttcgctta ttatggggat cagtttcagg gtttcaaggg aagcactcac
    19381 attgtcatca atcttcgcaa caaggatctc ggaaaaatga ataatcattt caagtgtatt
    19441 ggcattgtgg gacacccacg gcaccctact gcactgacaa cacatgaaat gctctaccgc
    19501 tggctgtgca caaaaggtta cgaggtcatc gttgagcaac aaatcgctca cgaactgcaa
    19561 ctgaagaatg tgaaaactgg cacgctcgcg gagattgggc aactggctga tctcgcggta
    19621 gtcgttggtg gcgacggtaa tatgctgggc gcgacgcgca cgctcgcccg ttacgatatt
    19681 aaagttattg gaatcaaccg tggcaacctg ggtttcctga ctgaccttga ccccgataac
    19741 gcccagcaac aattagccga tgtgctggaa ggtcactaca tcagcgagaa acgttttttg
    19801 ctggaagcgc aagtctgtca gcaagattgc caaaagcgca tcagcaccgc gataaatgaa
    19861 gtggtacttc atccaggcaa agtggcgcat atgatagagt tcgaagtgta tatcgacgag
    19921 atctttgcgt tttctcagcg atctgatggg ctaattattt cgacgccaac aggctccacc
    19981 gcctattccc tctctgcggg cggtcctatt ctgaccccct ctctggatgc gattaccctg
    20041 gtgcccatgt tcccgcatac gttgtcagca cgaccactgg tcataaacag cagcagcacg
    20101 atccgtctgc gtttttcgca tcgccgtaac gacctggaaa tcagttgcga cagccagata
    20161 gcactaccga ttcaggaagg tgaagatgtc ctgattcgtc gctgtgatta ccatctgaat
    20221 ctgattcatc cgaaagatta cagttatttc aacacattaa gcaccaagct cggctggtca
    20281 aaaaaattat tctaatttta cgccagcctc tttactgtat ataaaaccag tttatactgt
    20341 acacaataac agtaatggtt tttcatacag gaaaacgact atgttggcac aactgaccat
    20401 cagcaacttt gctatcgttc gtgagcttga gattgatttt catagcggca tgaccgtaat
    20461 aactggcgaa accggtgcgg gtaaatctat tgcaatagat gccctcggtc tttgtctcgg
    20521 tggtcgcgct gaagccgaca tggtgcgtac cggcgctgct cgcgctgacc tgtgcgcccg
    20581 tttttctctg aaagatacgc cagcggccct gcgctggctg gaagaaaacc agcttgaaga
    20641 cgggcatgaa tgtttgcttc gtcgcgtgat cagcagcgat ggtcgctccc gtggtttcat
    20701 caacggtaca gctgttcctc tgtcacaact gcgcgaactg ggtcagttgc tgattcagat
    20761 ccatggtcag cacgctcatc agttactcac caaacccgag caccaaaaat tcctgcttga
    20821 tggctatgcc aatgaaacct ctctactcca ggaaatgacc gcacgttatc agttgtggca
    20881 tcaaagctgc cgtgacctcg cgcatcatca acagttaagt caggaacgcg ctgcccgtgc
    20941 ggaactgctg caataccaat taaaagaact taacgaattt aatccgcagc ccggagagtt
    21001 tgagcaaatc gacgaagagt acaaacgtct ggcgaacagc ggtcaattgc tgaccaccag
    21061 ccagaatgca ttggcattaa tggccgacgg tgaagacgca aacctgcaaa gtcagcttta
    21121 cacggctaaa caactggtga gcgaattgat tggcatggac agcaaactgt ccggcgtact
    21181 tgatatgctg gaagaagcta ccatccagat tgttgaagcc agcgacgaac tgcgccacta
    21241 ctgcgatcgt ctggatctcg atcccaaccg actgtttgaa cttgaacagc gcatctcaaa
    21301 acagatttcg ctggcacgta aacatcacgt cagccctgag acattgccac agtattacca
    21361 gtcgctactg gaagaacagc agcaactgga cgatcaggcc gactcacaag aaacgctcgc
    21421 actggcggta acgaaacatc atcagcaggc actggaaacg gcgcgcgcat tacaccagca
    21481 acgccagcat tatgcagaag aacttgcaca gctgatcacc gacagtatgc atgcgctctc
    21541 aatgccgcat gggcagttta cgattgatgt caaatttgac gagcatcacc tgggcgctga
    21601 cggcgccgat cgtattgagt ttcgggtaac caccaaccca ggtcagccaa tgcagcctat
    21661 tgccaaagtc gcatccggtg gtgaattgtc ccgcatcgca ttggcaattc aggtcatcac
    21721 ggcgcgtaaa atggaaaccc cggcactgat ttttgatgaa gtggatgtag ggattagcgg
    21781 tccaacagcg gcagttgtcg gcaaactgct gcgtcaactc ggcgaatcaa ctcaggtgat
    21841 gtgtgttacc cacctgccac aagtcgcggg atgtggtcat caacactatt ttgtcagcaa
    21901 agaaaccgat ggtgcgatga cagaaacgca tatgcaatcc ctgaataaaa aaacgcggtt
    21961 acaagagctg gcgcgcctgc ttggtggcag tgaagtcaca cgtaacacac tggcgaatgc
    22021 gaaagaactg cttgcggcgt aaactttttt cctgcttcac ggtcagagta aacagcaaaa
    22081 cgccgtaaga ccggaaagca aaaggtttta aagtgatgaa aggtctatta tcatcggcat
    22141 attacagatg agccacgtac tgctcgggcc cgaaaaggaa tcaaatcact atgcgctgta
    22201 aaacgctgac tgctgcagca gcagtactat tgatgttgac cgcaggctgt tccactctgg
    22261 agcgagtggt ttaccgtcct gacatcaacc aggggaacta tctgaccgct aacgacgtat
    22321 ccaaaatacg tgttggcatg acgcaacaac aagttgcgta cgcattgggt acaccgctga
    22381 tgtccgatcc atttggtacg aatacctggt tctatgtctt ccgccagcaa ccaggtcatg
    22441 aaggtgtaac gcagcaaacg ctgacgctaa cctttaacag tagcggtgtg ttgaccaata
    22501 ttgataacaa acctgcgctg agtggtaact aataataaag ttgctctcaa agacgttaaa
    22561 aaaggtgctc aatgagcacc ttttttctgt ccgttattta ttcgctgatt tttctgctcg
    22621 ttgcctgcga agctctttcg gatcggcaat gagagggcga taaatctcca cccgatcgcc
    22681 atcatgcaca ctatcgctta attttgccgg acggctgtaa atgccgactt tgttttcagt
    22741 taaatcgata tcggtacgca attccagcaa gccactggcg cgaatagctt cttcaaccgt
    22801 cgcgccctcc tgcagcgtca cgcgctgcag gtactgcttc ttaggtagcg cataagccac
    22861 ctcaacggca attttacctg gcactgtaaa cctctttcgc acgaaccgta aaagcctgga
    22921 ccatattaga cgccagctct ttaaacacgc gaccaaaagc gagttcaatc aacttattgg
    22981 taaactcaaa gtcgagatga aactcgatac gacacgcctc ctggctcagc ggcgtaaact
    23041 tccatccacc aatcaatttc ttgaacggcc catccaccag attcataaga atactttggt
    23101 tactggtcaa ctggttgcgg gtagtaaacg ttttgctgat cccagcctta gagacatcta
    23161 ccgccgcagt catctgccca ggagtggact ccagaatccg acttccggta caacccggca
    23221 aaaactgagg ataagactga acgtcattca ctaactgata catttgctcc gcgctgtagg
    23281 gtaccagtgc ggtccggcta atctgaggca taacaatttc catcaacaaa aatccaacaa
    23341 ataatatcat ttatcctgca gtaaaaaaaa cgctatcccg gcgctgggta acatccggtt
    23401 catgctaaga tagagccttg tcccccgcag gattgatatg gggtattttc gatttcagat
    23461 taccgatgat tcacgacgct tatgacgaag aaaaaagcac ataaacctgg ttcagcgacc
    23521 atcgcgctta acaagcgcgc ccgtcacgaa tactttattg aagaagagtt cgaagcggga
    23581 cttgccctgc aaggctggga agttaaatcc ctgcgcgcag gaaaagccaa tattagcgac
    23641 agctatgtcc ttctgcgcga cggagaagcg ttcctgtttg gcgcaaacat cacgccaatg
    23701 gctgtggctt ctacgcatgt ggtgtgcgat cctacccgta cccgcaagtt acttctcaac
    23761 cagcgcgaac ttgattcgtt gtacggtcgc gtcaatcgag aaggctatac cgtagtggcg
    23821 ctctccctgt actggaaaaa tgcctggtgc aaagtgaaaa tcggcgtcgc caaaggtaag
    23881 aaacagcacg ataaacgttc agatatcaaa gaacgcgaat ggcaggtgga taaagcgcgt
    23941 atcatgaaaa acgcccaccg ttaaacctgc actccaattc ttgacctgtt cctcaccgcg
    24001 cctccctctc cggcggcgcg aatgaacatc ttattggcta tcacatccga caaaaatgtt
    24061 gccatcccat tgcttaatcg aataaaaatc aggctacatg ggtgctaaat ctttaacgat
    24121 aacgccattg aggctggtca tagcgctcat aaatctggta tacttgccgt tacacattgg
    24181 ggctgattct ggattcgacg ggatttgcga aacccaaggt gcatgccgag gggcggttgg
    24241 cctcgtaaaa agccgcaaaa aatagtcgca aacgacgaaa actacgcttt agcagcttaa
    24301 taacctgctt agagccctct ctccctagcc tccgctctta ggacggggat caagagaggt
    24361 caaacccaaa agagatcgcg tggaagccct gcctggggtt gaagcgttaa aacttaatca
    24421 ggctagtttg ttagtggcgt gtccgtccgc agctggcaag cgaatgtaaa gactgactaa
    24481 gcatgtagta ccgaggacgt aggaatttcg gacgcgggtt caactcccgc cagctccacc
    24541 actttaagaa ggactacaac cggacagtag caataaatac agccacttac ggacacaagc
    24601 cagacagtag tcggactgaa aaaagacaaa aatatacacg tgaaatgcac gtgcacttct
    24661 aaaaaacccc agttatcacc gactggggtt tttcaatttg taactaaggg taacaacccc
    24721 cccgtttttt tcatctcact caccttgata ctgtttattt gtacagttaa aaataatact
    24781 gtatacaaac acagtataga gggactttta tgcgtattga gatctgcata gccaaagaaa
    24841 aaat
//
LOCUS       DAZFBR010000072        24601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFBR010000072 DAZFBR010000000
VERSION     DAZFBR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.072"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            52..1044
                     /gene="tehA"
                     /locus_tag="ACHJZ2_003091"
     CDS             52..1044
                     /gene="tehA"
                     /locus_tag="ACHJZ2_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO4569163.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            1041..1634
                     /gene="tehB"
                     /locus_tag="ACHJZ2_003092"
     CDS             1041..1634
                     /gene="tehB"
                     /locus_tag="ACHJZ2_003092"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO4569164.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            1938..2606
                     /gene="ydcL"
                     /locus_tag="ACHJZ2_003093"
     CDS             1938..2606
                     /gene="ydcL"
                     /locus_tag="ACHJZ2_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO4569165.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(2641..3852)
                     /gene="ydcO"
                     /locus_tag="ACHJZ2_003094"
     CDS             complement(2641..3852)
                     /gene="ydcO"
                     /locus_tag="ACHJZ2_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO4569166.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
     gene            3905..4441
                     /gene="sutR"
                     /locus_tag="ACHJZ2_003095"
     CDS             3905..4441
                     /gene="sutR"
                     /locus_tag="ACHJZ2_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO4569167.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            4514..6475
                     /gene="rlhA"
                     /locus_tag="ACHJZ2_003096"
     CDS             4514..6475
                     /gene="rlhA"
                     /locus_tag="ACHJZ2_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO4569168.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(6567..6797)
                     /gene="yncJ"
                     /locus_tag="ACHJZ2_003097"
     CDS             complement(6567..6797)
                     /gene="yncJ"
                     /locus_tag="ACHJZ2_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO4569169.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            7019..7195
                     /gene="hicA"
                     /locus_tag="ACHJZ2_003098"
     CDS             7019..7195
                     /gene="hicA"
                     /locus_tag="ACHJZ2_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO4569170.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            7241..7657
                     /gene="hicB"
                     /locus_tag="ACHJZ2_003099"
     CDS             7241..7657
                     /gene="hicB"
                     /locus_tag="ACHJZ2_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO4569171.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            7736..9142
                     /gene="ydcR"
                     /locus_tag="ACHJZ2_003100"
     CDS             7736..9142
                     /gene="ydcR"
                     /locus_tag="ACHJZ2_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4569172.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            9387..10532
                     /gene="ydcS"
                     /locus_tag="ACHJZ2_003101"
     CDS             9387..10532
                     /gene="ydcS"
                     /locus_tag="ACHJZ2_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO4569173.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            10550..11563
                     /gene="ydcT"
                     /locus_tag="ACHJZ2_003102"
     CDS             10550..11563
                     /gene="ydcT"
                     /locus_tag="ACHJZ2_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4569174.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            11564..12505
                     /gene="ydcU"
                     /locus_tag="ACHJZ2_003103"
     CDS             11564..12505
                     /gene="ydcU"
                     /locus_tag="ACHJZ2_003103"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4569175.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            12495..13289
                     /gene="ydcV"
                     /locus_tag="ACHJZ2_003104"
     CDS             12495..13289
                     /gene="ydcV"
                     /locus_tag="ACHJZ2_003104"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4569176.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            13311..14735
                     /gene="patD"
                     /locus_tag="ACHJZ2_003105"
     CDS             13311..14735
                     /gene="patD"
                     /locus_tag="ACHJZ2_003105"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO4569177.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(14832..14927)
                     /gene="yncL"
                     /locus_tag="ACHJZ2_003106"
     CDS             complement(14832..14927)
                     /gene="yncL"
                     /locus_tag="ACHJZ2_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO4569178.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            15122..15295
                     /gene="ortT"
                     /locus_tag="ACHJZ2_003107"
     CDS             15122..15295
                     /gene="ortT"
                     /locus_tag="ACHJZ2_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO4569179.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            15381..15614
                     /gene="ydcY"
                     /locus_tag="ACHJZ2_003108"
     CDS             15381..15614
                     /gene="ydcY"
                     /locus_tag="ACHJZ2_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO4569180.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(15615..16064)
                     /gene="ydcZ"
                     /locus_tag="ACHJZ2_003109"
     CDS             complement(15615..16064)
                     /gene="ydcZ"
                     /locus_tag="ACHJZ2_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4569181.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNSLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(16061..16579)
                     /gene="mddA"
                     /locus_tag="ACHJZ2_003110"
     CDS             complement(16061..16579)
                     /gene="mddA"
                     /locus_tag="ACHJZ2_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO4569182.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            16761..17798
                     /gene="curA"
                     /locus_tag="ACHJZ2_003111"
     CDS             16761..17798
                     /gene="curA"
                     /locus_tag="ACHJZ2_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO4569183.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            17996..18661
                     /gene="mcbR"
                     /locus_tag="ACHJZ2_003112"
     CDS             17996..18661
                     /gene="mcbR"
                     /locus_tag="ACHJZ2_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO4569184.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(18697..20799)
                     /gene="pqqU"
                     /locus_tag="ACHJZ2_003113"
     CDS             complement(18697..20799)
                     /gene="pqqU"
                     /locus_tag="ACHJZ2_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO4569185.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            21041..22102
                     /gene="yncE"
                     /locus_tag="ACHJZ2_003114"
     CDS             21041..22102
                     /gene="yncE"
                     /locus_tag="ACHJZ2_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO4569186.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(22217..23716)
                     /gene="ansP"
                     /locus_tag="ACHJZ2_003115"
     CDS             complement(22217..23716)
                     /gene="ansP"
                     /locus_tag="ACHJZ2_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO4569187.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAIALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            23983..24600
                     /gene="yncG"
                     /locus_tag="ACHJZ2_003116"
     CDS             23983..24600
                     /gene="yncG"
                     /locus_tag="ACHJZ2_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO4569188.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILKRNEII"
BASE COUNT         6243 a         5988 c         6331 g         6039 t
ORIGIN      
        1 ataaaatgca tttcaaatat actttataaa ttaaacaaaa tgagtaagaa gatgcagagc
       61 gataaagtgc tcaatttgcc ggcaggctac tttggtattg tgttggggac gatagggatg
      121 ggatttgcct ggcgctatgc cagccaggtt tggcaggtca gccactggtt aggggatggg
      181 ctggtgattc tggcgatgat catctgggga ttattgacta gcgcatttat tacccgactc
      241 atacgctttc cgcatagcgt gctggcggaa gttcgccatc cagtgctgag cagttttgtg
      301 agtttgtttc ctgcaacgac gatgctggtg gcgattggtt ttgttccgtg gtttcgccca
      361 ctggcggtgt gcctgttcag ttttggtgtc gtggttcagt tggcttatgc cgcctggcaa
      421 actgcgggat tatggcgcgg atctcaccct gaagaagcta ctacgcctgg actgtatctg
      481 ccgacagttg ccaacaactt tatcagcgca atggcctgtg gtgcgttggg ctacaccgac
      541 gccggtctgg tgtttttagg cgcaggcgtt ttctcatggc taagccttga accggtgatc
      601 ttgcagcgtc tgcgtagttc gggagaatta cccacggcac tgcggacatc actcggcatt
      661 cagctcgctc ctgcgctggt ggcctgtagt gcctggctga gcgtcaacgg cggcgagggt
      721 gacacgctgg cgaaaatgct tttcggttat ggactgctgc aactgctgtt tatgctacgt
      781 ctgatgccat ggtatctctc ccagccattt aatgcttcat tctggagttt ctcgttcggc
      841 gtatctgcac tggcaaccac cggtttgcat ctggggagtg gcagcgataa tggatttttc
      901 catacgctgg cggtgccgct gtttatcttt accaatttta ttattgcaat actgctcatc
      961 cgtactttgg cgcttctgat gcagggaaaa ttgttagtca gaaccgagcg cgccgtttta
     1021 atgaaagcag aggacaaaga atgatcattc gtgacgaaaa ttattttact gataaatatg
     1081 aattaacccg cacacactct gaagtactgg aagcggtgaa agtggttaaa ccgggtaaaa
     1141 cgctggatct gggctgtggc aatggtcgta acagtcttta cctggcagcc aatggttatg
     1201 atgttgacgc atgggataaa aatgccatga gtatcgccaa cgtcgagcgc attaaatcca
     1261 ttgaaaatct ggataattta cacacccggg tcgttgatct gaataacctc acatttgatg
     1321 gagagtacga ttttattctt tcgactgtgg tgctgatgtt ccttgaggct aaaaccatcc
     1381 cagggctgat tgccaatatg caacgttgca ctaaacctgg cggttacaac ctgattgtgg
     1441 cggcgatgga taccgctgat tatccatgta ccgtcggctt cccgtttgcc ttcaaagagg
     1501 gagaattacg tcgatattac gaaggctggg agatggtgaa atacaatgaa gacgtcggcg
     1561 agctgcaccg caccgacgcc aacggtaatc gtattaaact gcgtttcgcc acgatgctgg
     1621 cacgtaaaaa atgacccggt aagaacaaag cgcgtgaaaa ttccccacgc tgagatggtt
     1681 tactgtcctt cttttcggta agcatatttt ttatcgaagg gatgtgaaat taatcacagt
     1741 agtcgaagtt ttttagcagc ttaacttact gaaatttaac tactgatgat tgacttagcc
     1801 ccttttgcgg cattgactat gtcgtctgaa aaggggctaa aaaaatttat tttcaccaac
     1861 actttttttg ccacaacacg aagcggcgct ttttgctata acttaaaaag taatataatc
     1921 atctcaggaa actattcatg cgtaccacat catttgcgaa agttgcagct ttatgcggct
     1981 tgttggctct gtctggttgt gcctctaaaa tcacccagcc agataaatat tctggttttt
     2041 taaacaatta ctctgattta aaagaaacaa cctcggctac aggtaaaccg gttttacgtt
     2101 gggtagaccc gagttttgat caaagcaaat atgacagcat cgtctggaac ccaatcactt
     2161 attatccggt accgaaacct tcgacccagg tagggcagaa agttctggat aaaattttga
     2221 actataccaa caccgaaatg aaagaagcga tagcgcagcg taaaccactg gttaccaccg
     2281 cggggccgcg tagtctgatt ttccgtgggg ccattaccgg tgtagatacc agcaaagaag
     2341 ggctgcaatt ctatgaagtg gttcctgttg cattagtggt tgcggggacg caaatggcta
     2401 caggccaccg taccatggat actcgcttgt attttgaagg tgagctgatt gatgcagcga
     2461 ctaataaacc ggttatcaaa gtcgttcgtc agggcgaagg taaagacctg aataacgaaa
     2521 gtacgccaat ggctttcgaa aatattaaac aagttattga tgacatggcg accgatgcca
     2581 ccatgtttga cgttaacaaa aagtagttcc agacgcgcca tcgttagatg gcgcttttta
     2641 ttatcggttt ctgtcagcga ttaaattcaa caccacgtaa caaacgcctc cggcaattaa
     2701 tccccaaaac gcagaaccaa tcccgaccag cgtcaatcca cttgccgtta ccagaaatgc
     2761 caccaccgcc gcgtcgcgct cacgctcatt atgcagcgcc tgatacaaac tgccgccaat
     2821 ggtactcaac agcgccagac ctgccagcat ctggatccaa cttacgggca gggcagccat
     2881 catcccggta atggcactac caaacagacc tgcgagcaaa tagaaaatgc ctgcaacggc
     2941 agctgccagc caacgttgat ctttatccgg atgcgcttcc gggctttggc aaatagccgc
     3001 ggtgattgcc gcaataccga cggaataaac gccgaaaggg gaaaaaacca gtgccagcaa
     3061 tccagtaaac acaatcaacg gcgaaacagg agccgaatac ccggctgctt tcattgctgc
     3121 gatacccggt gcgttttgag atgccatcgt caccagaaaa agggggagtg caacgctcag
     3181 gctgtgagca aacgaaaaat caggggtaat ataagtgggg agaacgggtt taaagacaac
     3241 atcagttgtg acaacgtcac cttgcgcgat aacgatcacg atcccaataa tcatcgcggc
     3301 aattaccgca tagcgcggcg caacggcctt ggttgccagc cataccagca acatacttcc
     3361 acacaacgta aattgaccgt ccagactggc aaacgcctgt aaaccaaagc gtaataaaat
     3421 cccggcaagc attgctgccg caagcgagtg cggaataatg cgcatcagac gagcaaagag
     3481 tcccgttatg ccgcagagga ctattaacgc gttggtgaca ataaaaacgc cgatggcttc
     3541 gttaagtgtt agtccctgca atccggtgac caacaaagcc gcgccaggcg ttgaccatgc
     3601 ggtgagaaca ggtacgcgat accataatgt cagagtcagc gtactgacgc ccattgccag
     3661 ccccagcgcc gtcatccagc cagagatttg tgcagtggtg gctccggcga caatcgctgc
     3721 ttgccagatt attgccgctg aactggcgta gccaattaat accgccagaa accccgccag
     3781 tagcgtgggt ggaggaacag aaaacagacg catagtcacc tcgtgcgtta tagcgtacag
     3841 gcaccgtagc atttgtccgt tataacgcac aagtgataaa cttccgtttt gccggaggag
     3901 tcgcatggaa aatctcgctc gctttttatc caccacactt aaacaactac gtcagcagcg
     3961 cggctggagt ctttcgcgac tggcagaagc gacgggcgtt tctaaagcaa tgttaggaca
     4021 gattgagcgt aatgagtcca gcccgacggt cgcgacatta tggaaaattg ccactgggtt
     4081 gaacgtgcca ttttcgacat ttatttctcc gccacagtcc gctacgcctt cagtttatga
     4141 tccacaacag caggcgatgg tgatcatttc gctgtttcct tatgatccac aactctgctt
     4201 tgaacacttc tcgattcaga tggcacccgg tgcaataagc gaatcgacgc cccatgagaa
     4261 gggggtaatc gaacatgtcg tcgtcattga tggacaactt gatctgtgcg ttgatggtga
     4321 atggcagacc cttaattgtg gggaaggcgt tcgatttgct gcagacgtca cgcatatcta
     4381 tcgtaatggt ggggagcaaa ccgtacattt tcattccctc atccattacc cgcgcagtta
     4441 agcgggaaaa ctatttcgca acgtcgcgct tgtggctaaa atagccgcca tttttcagct
     4501 actggataag aatgtgaccg tatcttctca tcgacttgaa ctgttaagcc cggcacgcga
     4561 tgccgccatt gcccgcgaag ctattttgca cggtgccgat gctgtttata tcggcggccc
     4621 tggttttggt gcccgtcata atgccagtaa tagcctgaaa gatattgccg agctggtgcc
     4681 gtttgcccat cgttatggtg caaaaatttt cgtcacactt aacaccattt tgcatgatga
     4741 tgagctggaa cccgcgcaac ggctgattac tgacctctac cagaccggtg tcgatgcgct
     4801 gattgttcag gatatgggga ttctggaact tgatattccg ccgattgaac tgcacgccag
     4861 tacgcagtgc gacattcgta cagtagaaaa agcgaagttc ctctctgatg ttggcttcac
     4921 gcagattgtg ctggcgcgag agctgaatct tgatcagatc cgcgcgattc accaggccac
     4981 ggacgcgacc attgaattct ttattcatgg cgcactgtgc gtggcctatt cgggtcagtg
     5041 ctacatttct catgcgcaaa cagggcgtag cgccaaccgt ggcgattgct cgcaagcgtg
     5101 ccgtttgcca tacacattga aagacgatca ggggcgggtg gtttcctatg aaaaacatct
     5161 gctgtcgatg aaagataacg atcagactgc caacctcggc gcgctgattg atgctggcgt
     5221 acgttccttc aagattgaag ggcgttacaa agatatgagc tacgtgaaga atatcaccgc
     5281 ccattatcgc cagatgcttg atgccattat tgaagaacgt ggcgatctgg cgcgcgcttc
     5341 atcaggtcgt actgaacatt tctttgttcc atcgacggaa aagactttcc accgtggtag
     5401 cacagattat tttgtgaatg cccgtaaagg cgatattggc gcgttcgatt cgccgaaatt
     5461 tatcggcctg ccggtaggtg aagtagtgaa agtggcgaaa gatcatctcg atgttgccgt
     5521 taccgagcca ctggcaaatg gcgatggcct gaacgtgttg attaaacgtg aagtcgtcgg
     5581 ttttcgtgcc aatacggtcg agaaaaccgg agaaaatcag taccgcgtct ggcccaatga
     5641 aatgccagca gatttgcaca aaattcgccc gcatcaccca ctaaaccgta atcttgatca
     5701 taactggcag caggcactga caaaaacctc tagcgaacgt cgggtggcgg tagacattga
     5761 actgggcggt tggcaggaac aattgattct gactctcacc agtgaagagg gggtcagcat
     5821 aacgcatacg ctggatgggc agttcgacga agccaataac gcagaaaaag caatgaacaa
     5881 tctgaaggat ggtctggcaa aactggggca aaccctctat tacgcccgcg atgtgcaaat
     5941 taatttgccg ggggcgctgt ttgtaccaaa cagtctgtta aaccagttcc gccgtgaagc
     6001 tgccgacatg ctggatgctg cgcgtcttgc cagttaccag cgcggcagcc gtaaaccggt
     6061 tgctgatcct gcgccggttt atccgcaaac acatctgagt ttcctcgcga acgtttacaa
     6121 ccagaaagcg cgtgaatttt atcatcgcta tggtgtgcag ctgattgacg cggcgtatga
     6181 ggcacatgaa gagaagggcg aagtcccggt gatgatcacc aagcattgtc tgcgctttgc
     6241 ctttaatctg tgcccgaaac aggcgaaagg caatatcaaa agctggaagg cgacgccaat
     6301 gcaactggtt aacggcgatg aagtattaac gctaaagttt gattgccgcc catgcgagat
     6361 gcacgtcatt ggcaaaatca aaaatcacat actgaaaatg ccgttaccgg gaagcgtagt
     6421 ggcatccgta agtccggatg agctgctgaa aacattgccg aagcgaaaag ggtaaaacgc
     6481 cagttttctg gttactcaca acttattgaa tctgcatgat attgcctgcc gggtaaggcg
     6541 ttacgccgca tccggcatca aacgactcaa cgttgtcccg cttctggttt gcgcgatttt
     6601 tgccagtaaa aatgctcgcg caaaccttcc gccgactctt ccgccacagc acgcaattca
     6661 tcgctgtccg cttcatgacg cagctgatga tccacattct ttacccacac aaattcatgt
     6721 cctttgtgcc ctgccatgag ttgtcctgag aacagcgcac acgttaataa gacaaccgat
     6781 aacgccttcg taaacatcct gccacctttt tcttaccttt tgccgctatg atgccgatcg
     6841 tttcttgagg ttattattca gttttgcaaa ttagcgcaaa gaaattctgg aatcttcctt
     6901 cctgattttg cattgcattc tgccgttgcg gcgatttagt gctattttcg agcaaattac
     6961 acacggaggt aaacactaat gtttactttg ttgatatact cagccgcagg gaggcgatgt
     7021 gaaacaaagc gagttcagac gttggctcga atctcagggc gtcgatgtag cgaatggcag
     7081 caaccatttg aaactcaggt ttcatgggag gcgcagtgtc atgccgcgtc acccctgcga
     7141 tgagattaaa gaaccattgc gtaaagcaat cctgaaacaa ctcggtttga gttaatcgcc
     7201 aattaaaaag gttaatgaca tgcgagagac agtcgaaatt atgcgttatc ccgtcactct
     7261 tacacccgcg ccggaaggcg gttatatggt ttcttttgtg gatatccctg aagcgttgac
     7321 gcagggcgaa actgtcgctg aagcgatgga agcggcaaaa gatgctttac tgaccgcatt
     7381 tgatttttat tttgaagata acgagcttat ccctttacct tcgccattaa atagtcatga
     7441 tcactttatt gaagtacctt tgagcgtcgc ctctaaggta ttgctgttaa atgctttttt
     7501 acagtcagaa atcactcagc aagagttagc caggcgaatt ggcaaaccta aacaagagat
     7561 tactcgccta tttaacttgc atcatgcgac aaaaatcgac gccgtccagc tcgcggcaaa
     7621 ggcgcttggc aaagagttat cgctggtgat ggtttaatta cagttaacga aaagttgtca
     7681 tttttaacaa ctgatataga ctgccgaatc atctgcacat aattacgatt cgataatgaa
     7741 aaaataccag cagcttgcag aacaattacg cgagcagatt gcgtcgggta tctggcaacc
     7801 cggcgatcgt ttaccttcgt tgcgtgacca ggtggcgctt tctggcatga gctttatgac
     7861 tgtcagccat gcctatcagt tgctcgaaag tcagggatat attatcgcac gaccgcagtc
     7921 gggttattac gttgcgccac aggcaataaa aatgccgaaa gtgccagtca ttccagtcac
     7981 tcgagatgaa gcagtcgata tcaacactta tatttttgat atgttgcagg ccagtcgcga
     8041 tccgtcggtc gttccgtttg cctcggcctt tcccgacccg cgacttttcc ccctccaaca
     8101 actaaaccgc tcgctggcgc aggtaagcaa aaccgccacg gcgatgagcg tgattgaaaa
     8161 cttgccgcca ggaaacgcag aactgcgtca ggctattgcc cgtcgctatg ccttacaggg
     8221 gatcaccatt tctcctgatg aaattgtcat cactgccggg gcgttagagg cattaaacct
     8281 cagtttgcaa gcggtaactg aaccgggcga ttgggtgata gtagagaatc cttgtttcta
     8341 cggtgcgttg caggcgctgg agcggctacg gctgaaggcg ttatcggtgg cgacggatgt
     8401 taaagaaggg atcgatcttc aggcgctgga actggcgttg caggagtatc cggtgaaagc
     8461 gtgctggctg atgactaata gccagaatcc actcggattt accttaacgc cgcaaaaaaa
     8521 agcacaactg gtggcgttgc tcaatcagta caacgtaacg ctgattgaag atgacgttta
     8581 cagcgaactt tattttggac gggaaaaacc gctgcctgcg aaagcgtggg atcgccacga
     8641 tggcgttttg cattgctctt cgttttcgaa atgtctggtg cctggttttc gtattggttg
     8701 ggtcgccgcc ggaaaacatg cacgtaaaat tcaacgcttg cagctgatga gtacgctttc
     8761 caccagctca ccgatgcagc ttgcgctggt ggattacctt tccacgcgcc gatacgacgc
     8821 ccatcttcgt cgcctgcgtc gccagcttgc ggaacgtaaa caacgtgcct ggcaggcact
     8881 gctgcgttat ctgcctgcgg aagtgaaaat tcatcataat gacagcggtt attttctctg
     8941 gctggagctc cccgagccgt tagatgccgg cgaattgagc ctggcggcac tgacgcatca
     9001 tatcagtatt gcgccaggta aaatgttttc taccggtgaa aactggtcac gtttttttcg
     9061 ttttaatacc gcgtggctgt ggggagaacg tgaagaacag gcggtaaaac aattaggcaa
     9121 acttattcaa gaacggctgt aatagcgttt aatttaattc ctcttagatt gggtaatatg
     9181 aatttcgaat agcagtcata tttcctaact ccttgtctat actccagaag ataaccttac
     9241 agacggcata atgcgcggta gctcacaacc tgattaaatt ttctcagggg cgaaggtgtg
     9301 cctgcaagcc gccgtctatg gttaaacaag gagatatttt tacggcacgg cggctgaaca
     9361 attaattacg acaggagtaa gaccttatga gcaagacatt tgcccgcagc agcctgtgtg
     9421 cgctcagcat gacaataatg accgctcacg ccgccgaacc gcctaccaat ttagataaac
     9481 cggaagggcg actggatatt atcgcctggc cgggatatat cgaacgcgga caaactgata
     9541 aacaatacga ctgggtaacg caattcgaaa aagagacagg ctgcgcggtg aatgttaaaa
     9601 ccgccgcgac ttccgatgag atggtcagtc tgatgaccaa agggggttac gatctggtta
     9661 cggcatccgg cgatgcctcg ctgcgtttga ttatgggtaa acgcgtgcag ccgattaata
     9721 ccgccttgat tcccaactgg aaaacgcttg atccgcgcgt ggttaaaggc gactggttta
     9781 acgttggcgg caaagtttac ggcacacctt accaatgggg gccgaacctg ctgatgtaca
     9841 acactaaaac cttcccgacg ccgccggata gctggcaagt ggtttttgtt gagcaaaatc
     9901 tgcaggacgg caagagcaat aaaggccgcg ttcaggctta tgatggccct atctacattg
     9961 cggacgctgc gttgttcgtt aaagccactc agccgcagtt gggcatcagc gatccgtatc
    10021 aactcaccga agaacagtac caggcggtgc tgaaagtgct gcgtgatcaa catagtttga
    10081 tccatcgcta ctggcatgac actaccgtgc aaatgagcga tttcaaaaac gagggtgtgg
    10141 tcgcttccag tgcctggccc tatcagacca acgccctgaa agccgaaggt cagcctgtcg
    10201 ctaccgtttt cccgaaggag ggcgttaccg gttgggctga caccaccatg ctacatagcg
    10261 aagcgaaaca tccggtttgc gcctacaaat ggatgaactg gtcattaacg ccaaaagtgc
    10321 agggcgatgt ggcggcctgg tttggctcgt taccagtcgt gccggaaggg tgtaaagcca
    10381 gttcgttatt aggcgagaaa ggttgtgaaa caaacggttt taactatttc gataaaatag
    10441 ccttctggaa aacgcctata gcagaagggg gcaagtttgt tccctacagt cgctggacgc
    10501 aggattacat tgccattatg ggcggtcgct aacttcgctg gggtgcttta tgacgtacgc
    10561 agtggagttt gacaacgtct cgcggttgta cggtgacgtg cgcgcagtag atggcgtcag
    10621 tattgcgata aaagatggtg agttcttctc tatgctgggg ccgtccggct ccggcaaaac
    10681 cacctgcctg cgcctgattg ctggcttcga acagctttcc ggcggggcta tctctatctt
    10741 tggtaaaccc gccagcaatc tgccaccgtg ggagcgggac gtgaatactg tctttcagga
    10801 ctacgcgcta tttccgcata tgtcgattct tgacaatgtc gcctatgggc tgatggtcaa
    10861 aggcgtgaat aaaaagcagc ggcacgcaat ggcgcaagag gcgctggaga aagtggcgtt
    10921 ggggtttgta cagcaacgta aaccgtcaca actttctggt ggtcagcgcc agcgggttgc
    10981 tatcgccaga gcattggtga atgaaccgcg cgtattgctg ttggatgaac cgctcggcgc
    11041 actggatctc aaattgcgtg agcagatgca gcttgagctg aaaaaactgc aacagtctct
    11101 cggtatcact tttatctgcg ttacccacga tcagggcgaa gcgttatcga tgtccgatcg
    11161 tgtggcggtt ttcaataatg gacgcattga gcaggtcgat tccccgcgcg atctctatat
    11221 gcgcccgcgc acgccgtttg ttgccgggtt cgttggtaca tcgaatgttt ttgatggact
    11281 gatggcagag aaactttgtg gcatgacggg aagcttcgcc ctgcggccgg aacatatccg
    11341 cctcaacacc cctggtgaac tgcaggccaa tggcacgatc caggcggtgc aatatcaggg
    11401 cgcggcaact cgttttgaac tgaaattgaa cggcggtgaa aaactgctgg tgagtcaggc
    11461 caatatgaca ggcgaagaac tgcctgccac gctcacgccc ggacaacagg tgatggtttc
    11521 ctggtcgcgt gatgtgatgg tgccgctggt tgaggagagg tgaatggcga tgaacgtatt
    11581 gcaatcacct tcacgtccag gtctgggtaa ggtgtccggt ttcttctggc ggaatccggg
    11641 gctggggctg tttttgctgc tgcttggccc gctaatgtgg tttggcattg tctatttcgg
    11701 ctcgctgcta acgctgttat ggcagggctt ttataccttt gacgatttca ccatgtcggt
    11761 aacgccggaa ctgacgctgg cgaacatccg tgcgctgttt aatccggcga attacgacat
    11821 cattctccgc acgctgacta tggctgtggc agtcactatc gccagcgcca ttctggcttt
    11881 tccaatggcg tggtatatgg cgcgctatac cagcgggaaa atgaaagcgt ttttttatat
    11941 tgcggtaatg ttgccgatgt gggcgagcta cattgttaaa gcctatgcct ggacgttatt
    12001 gctggcaaaa gatggcgtgg ctcagtggtt tttacaacat cttgggctgg aaccactgct
    12061 gacagcgttc cttacattac ctgcggtggg cggcaatacg ctgtcaactt ccgggctggg
    12121 gcgctttctg gtgtttctct atatctggct gccgttcatg atcctgcccg tgcaggcggc
    12181 gcttgagcgt ttgccgccgt cattgttgca ggcgtcagct gatctcggcg cacgtccacg
    12241 acaaaccttt cgctatgtgg tgttgccgct ggcgatcccg ggtattgccg ctggctctat
    12301 ctttaccttc tcactcacac tgggcgattt tatcgtcccg cagctggttg gcccgccagg
    12361 atattttatc ggcaatatgg tttattccca gcagggggcg attggcaata tgccgatggc
    12421 ggcggcattc accctggtgc cgattgttct tatcgcactg tacctggcgt tcgtgaaacg
    12481 tctgggagcg tttgatgcac tctgaacgcg caccgttttt cctcaaactg gcggcctggg
    12541 gcggcgttgt tttcctacat tttcccatcc tgataatcgc cgcctatgcg tttaacactg
    12601 aagatgcggc gtttagtttt ccaccgcagg gcctgacgct gcgctggttt agcgtggcag
    12661 cacagcgtag tgatattctt gatgccgtga cactgtcact taaagtggcg gcgctggcga
    12721 cattaatggc gctggtgtta gggacgctgg cagctgccgc gctgtggcga cgagactttt
    12781 tcggcaaaaa cgccatttcg ctgttactgc tgctgcccat tgcgctgccg ggcattgtca
    12841 ctggtctggc gttattaacc gcctttaaaa ccatcaatct ggagccggga tttttcacca
    12901 tcgtggtcgg tcatgcgact ttttgtgtag ttgtggtgtt taacaatgtc atcgcccgtt
    12961 ttcgccgcac ctcatggagt ctggttgagg cgtcaatgga tcttggggcc aatggctggc
    13021 aaaccttccg ctacgtagtg ttgccgaatc tcagttcggc gttactggca ggaggaatgc
    13081 tggcgtttgc cttgtcgttc gatgaaatca tcgttacgac ctttacggca ggtcatgaac
    13141 gaacgttacc gttgtggttg ctcaatcagc ttgggcgacc gcgtgatgta ccggtaacta
    13201 acgtggtggc actgctggtt atgttggtaa caaccttgcc gatcctggga gcctggtggc
    13261 taacccgcga aggcgacaat ggtcaataac cactgataca ggaatatgct atgcaacata
    13321 agttactgat taacggagaa ctggttagcg gcgaagggga aaaacagcct gtctataatc
    13381 cggcaacggg ggacgtttta ctggagattg ccgaggcatc cgcagagcag gtcgatgctg
    13441 ctgtgcgcgc ggcagatgca gcatttgccg aatgggggca aaccacgccg aaagtgcgtg
    13501 cggaatgtct gctgaaactg gctgatgtta tcgaagaaaa tggtcaggtt tttgccgaac
    13561 tggagtcccg taattgtggc aaaccgctgc atagtgcgtt caatgatgaa atcccggcga
    13621 ttgtcgatgt ttttcgcttt ttcgcgggtg cggcgcgctg tctgaatggt ctggcggcag
    13681 gtgaatatct tgaaggtcat acttcgatga tccgtcgcga tccgttgggg gtcgtggctt
    13741 ctatcgcacc gtggaattat ccgctgatga tggcagcgtg gaaactggct ccggcgctgg
    13801 cggcagggaa ctgcgtagtg cttaaaccat cagaaattac cccgctgacc gcgttgaagt
    13861 tggcagagct ggcgaaagat atcttcccgg caggcgtgat taacgtactg tttggcagag
    13921 gcaaaacggt gggcgatccg ctgaccggtc atcccaaagt gcggatggtg tcgctgacgg
    13981 gctctatcgc caccggcgag cacatcatca gccataccgc gccctccatt aagcgtactc
    14041 atatggaact tggtggcaaa gcgccagtga ttgtttttga tgatgcggat attgaagcag
    14101 tggtcgaagg tgtacgtaca tttggctatt acaatgccgg acaggattgt actgtggctt
    14161 gtcggatcta cgcgcaaaaa ggcatttacg atacgctggt ggaaaaactc ggtgctgcag
    14221 tggcaacgtt aaaatctggt gcgccggatg acgagtctac ggagcttgga cctttaagct
    14281 cgctggcgca tctcgaacgc gtcagcaagg cagtagaaga ggcgaaagcg acagggcaca
    14341 tcaaagtgat cactggcggt gaaaagcgca agggtaatgg ctattactat gcgccgacgc
    14401 tgctggctgg cgcattacag gacgatgcca tcgtgcaaaa agaggtattt ggtccagtag
    14461 tgagtgttac gctcttcgac aacgaagaac aggtggtaaa ctgggcgaat gacagccagt
    14521 acggacttgc atcttcggta tggacgaaag atgtgggcag ggcgcatcgc gtcagtgcac
    14581 ggctgcaata tggttgtacc tgggtcaata cccatttcat gctggtaagt gaaatgccgc
    14641 acggtgggca gaaactttct ggttacggca aggatatgtc actttatggg ctggaggatt
    14701 acaccgtcgt ccgccacgtc atggttaaac attaaggata atattgcaga tcgtaagagt
    14761 atgagatgat cttgcgtact gtcagagcac tgatttctct gacagtacgt gctgcgtgca
    14821 aaatcactac atcaaataaa ccagccaaat ctcatagaga taagagtaaa caaaccaaca
    14881 gcagcaaaga catttatcag tactacagtt ctactggaaa cattcatttt tgccaccttt
    14941 tatcattgcc cctatgtgta tagtacgggt taagaaaatc cgcaataata ctcattctga
    15001 agatggataa gggcaagttg ctgtttgatg tttttttctc aaaccggtta aaaaacttga
    15061 tgcacgtcaa aaaatgacgc atatttgcgc gcgttttatt catctggctg gacgcccgta
    15121 catgtctctc tatcaacaca tgcttgtttt ttatgcggtt atggcagcaa tcgcatttct
    15181 tatcacttgg tttctttctc acgataagaa acgcatccgt ttcttaagcg cctttctggt
    15241 gggggcaaca tggccgatga gtttcccggt ggcgctgttg ttttcactgt tttaaatctt
    15301 actcactggc ttgacggcac cacgcagact tatatcattt ggatgaatcg ataaatttca
    15361 caagtggcta aggagaaagt atgtcgcatc tggatgaagt catcgcgcgc gtggatgccg
    15421 cgattgaaga gagcgtcatt gcccatatga acgaattatt aatcgccctg agcgatgacg
    15481 cggagttaag tcgggaagat cgctacaccc agcagcagcg tttgcgcacc gctattgctc
    15541 atcacggtcg caagcataaa gaagatatgg aagcgcgcca tgaacagtta accaaaggcg
    15601 gcaccatcct gtgattaaaa tgagcgtctg gctaccaacc aggcgccaac cacaagtaaa
    15661 attgcgccac aaattgggcc gaacaacgcc cgtagtggta cgccatgact gcggaagata
    15721 tccagaacca gaccgccaat cagctggctg gcgaccagta ctgcaatagt tgtcgccgct
    15781 cccacattct gatatccgct gatactggcg aagacaaaaa acgatcccag caagccaggt
    15841 ataagtgtcc accagcgcac gctggacacc agttcgccaa accccgccat accttgttta
    15901 aaccataaaa tagaaacaaa gagcacaatc cccaccagtg agttcagcaa catggcgatg
    15961 agaatggtag aggaggtctg ggtgatgcgc accatcagcg agttctggac taccagacca
    16021 attccggcgg caatcaaaaa ggcaagggtg agcgactgat tcatccaatc gcgtccggtt
    16081 cagtgcgttc gtcgagttgc aactgcataa atgtcagatc cagccagcgc ccaaatttag
    16141 tgcccacctg cggcatttgt gcggtgacga caaatcccag cgactggtgc agatgcagcg
    16201 aggcctgatt ttgcgattcg atcccggcga ccatgacatg cttcccgcaa tctcgcgctt
    16261 catcaatcaa tcggcttaac aatttacgcc ccagaccttt gccctgatga tcgggatgga
    16321 cataaaccga atgttccacg gtatggcgaa aaccatcgaa actacgccag tcgccaaatg
    16381 aggcatatcc cgtcactacg ccatcttctt cacttaccag caccggataa cctgctatgg
    16441 tccgcgcttc aaaccaggca atgcggttat ctgcatccac cgtttggtca ttccagatag
    16501 ccgccgtata caacacggcg tggttataaa tttccgcaat ggcagcacag tcggctttgc
    16561 gggcaaaacg gatggacatg tctggcctca ataaaataat gatgatgatg ttatcaatac
    16621 tatcgaaaac agcccgaagc aacggattcc tcgatgaaat tattgcgtgt tgcagagggg
    16681 ggggtgacgg ctgcaaacaa aaccaatcct gcgagcctgc tactattgat ggattcatgc
    16741 caaaagcaca ggagagcatt atggggcaac aaaagcagcg taatcgacgt tgggttctgg
    16801 cctcgcgtcc acatggcgca ccggttccgg agaatttccg tcttgaagaa gatgatgtcg
    16861 ccacaccggg tgaaggacag gtgttactgc gcacagttta tttgtccctg gacccgtata
    16921 tgcgtggacg tatgagcgat gagccatctt attcaccgcc ggttgatatt ggcggcgtga
    16981 tggtcggcgg tacggtgagc cgtgtcgtgg agtcgaatca tcctgattat cagtctggcg
    17041 actgggtgct gggctacagt ggatggcaag actatgacat atccagtggt gatgatctgg
    17101 tgaaacttgg cgatcatccg caaaatccat cgtggtcgct gggtgtgcta gggatgccag
    17161 gctttaccgc ttatatgggc ctactggata tcggtcagcc taaagagggc gaaacgttgg
    17221 tggtagctgc ggcgacagga ccagtggggg cgacggtggg gcaaatcggc aaacttaaag
    17281 gttgcagagt ggtgggggta gccggtggcg cggaaaaatg ccgccatgct accgaggtgt
    17341 taggcttcga tgtttgtctt gatcaccacg cggatgattt tgccgaacaa ctggcgaaag
    17401 cgtgcccaaa aggtattgat atctattatg aaaacgtggg cggtaaggta ttcgatgcgg
    17461 tgctaccgtt acttaataca tctgcgcgca ttcccgtctg cggattagtg agcagctata
    17521 acgctacaga gctaccaccc ggtccggatc gtttacctct gttgatggct acagtgctga
    17581 aaaaacgtat tcgcttgcaa ggttttatta tcgctcagga ttatggtcac cgcatccatg
    17641 agtttcagag ggagatgggg caatgggtga aagaggataa aatccactac cgcgaagaaa
    17701 ttactgacgg tttagagaat gcgccacaga cgtttatcgg cctgctgaag ggtaaaaact
    17761 tcggcaaagt ggtgatccgc gtggcgggtg atgattaaag tttcaacggc ggcgtaagcc
    17821 gccataaaaa tccaggcaaa aagcttattt tcagctttaa ttaactaacc acatcattgt
    17881 tctgctgcta ataacaacaa aggtgagtgg tttatgatta ttcactttaa tacaccaggt
    17941 gaattccttc tgccatgcag gcagggttgg acagaaaaca cgcgaaggaa ttaaaatgcc
    18001 tggaacggaa aaaatgaaac atgtcagttt gactctgcag gttgagaacg acctgaaaca
    18061 tcagcttagt attggcgcac taaaacctgg cgcacgcctg attactaaaa atctggcgga
    18121 gcaattaggt atgagtatta cacctgtgcg tgaagcatta ttacgtctgg tttcggtgaa
    18181 tgcgctttct gtcgcaccag cgcaagcatt tacagttccg gaagtgggga aacgtcaatt
    18241 agacgaaatc aatcggatcc gctacgaact ggaattaatg gcagttgctc tggctgttga
    18301 aaacctcacc ccgcaagacc tggcggaact ccaggaattg ctcgagaaat tacagcaggc
    18361 gcaagaaaag ggcgacatgg aacaaatcat taatgtaaac aggctatttc gcttagcgat
    18421 ttatcatcgc tcaaatatgc ccatcctgtg tgagatgatt gagcaactgt gggtcaggat
    18481 gggacctggt ttacattatc tctatgaagc gattaatcca gcggaattac gggagcatat
    18541 agaaaactat catctgttac tcgctgcgtt aaaagcaaaa gacaaagagg gatgcagaca
    18601 ttgtcttgct gaaattatgc aacaaaatat tgctatttta taccagcaat acaatcgtta
    18661 aagtaaatgc ccggcaaata ccgggcatca aggtaattac tcaaatcgcc atgcgatatt
    18721 cacgccgaca ccataatttc gcccgggcgc aggttcgtaa tatcgcccgt ttgactcatt
    18781 gacaatgaca gaaccaacgt attctttatc gaataaatta tcgacacgac caaacaaatc
    18841 cacagttaaa ttgtggtaat tgtatttata cccggtgaat aagccgacga gagtataaga
    18901 cggcgctttg gccgtatttt catcatctgc cataatatcg cccatataac gcgcttccat
    18961 gcctgcatac caaccatctt ccggtacata acctatcgac gcaaagccca tattacgggc
    19021 gatccctggc atccgattac cgttacaatc ctgttcgttg caaacattgc tgcgataggt
    19081 cgcatcaagc caggtccagg acgcgtttac gcgaaaatct cccgcgaaac gttgatccca
    19141 tgccagttca gcgccttgac gacgggtctt tccagcattt ttgtaagtcg tacgcccacc
    19201 gctactgcta tcgacaacaa tttcatcatc ggtgtcggtc tggaacaatg cgagactaag
    19261 cagcccatca ccaatacgcg ttttactgcc gatctcaatt gtatcgttgg tggatggttt
    19321 taaaccaaag ttcataccgc tttgcccatc agcgcgataa gacagctcat taatcgtcgg
    19381 cgtttcaaaa ccacgcccgg ctgccagata gatattccag gcatcggtca ttgcatattt
    19441 taacgatccg gctggtagcc atttgtgata actggcatca ccgctgtcat cgccgttacc
    19501 cggagtaacg taatggtcgt tggaatcaaa ccacacggag ctgtagcgca cgccagcatc
    19561 cagcgacagt ttttcgctca gctgccactg cgtctgtaaa tagggatcga tgttccacat
    19621 cagattgcgt tcgtcgcgac gcaactcacc tttttgcccg tactccggca tgccgctatt
    19681 caggcggaag ttattgtagc ccttgcggtt ttcactcatg ttttcgtagt tcaggccggt
    19741 agtgaacgtg accggaacgc ccagttcacc acggtgtgtc cagcggctgt ctattccctg
    19801 gtaatggcgt tgcagggtaa tcacgctgcc cgcatgtgac gggttaagtt gtggtgccat
    19861 gggtattgac tggtactggg tcgtttctcg ctctccggca tacatcatca cactcatatc
    19921 atcccgcgaa ctcaggctac gttcatagcg caacccagcc tgagtttgct tgatggtttt
    19981 tcgcgtgtcg tactgttctg cacgaggcgc ttgttgcgga ttcgctttcc attctgcttt
    20041 ggttagccca cctgggtcat ctgctttgat atccacgcta ttgaaaatca ggcttaattt
    20101 gctggcgtca tcaatgcgta cgcccagttt ggcattagct aaatttttct gtgcgccact
    20161 atggtcacga tagccgtggg tcgtaaaacg cgtggttgag acggtgtaat caacatcgcc
    20221 aggctgtgtg ccgtctcccg ttgcgcccgt tgctttcagc ccatagcgcc agctgccaaa
    20281 actgccgtag taactactgg cttcaatggt tggtggctgt tgcccggtct gggtggtgac
    20341 attcattaca ccgccagacg cgttgccata cagggcagag aaggggccac gcagcacttc
    20401 cacattttgc acactgctta aatcgatgtt ggatgtttgc ccttgcccgt cgggcatggt
    20461 agcgggaata ccgtccacat acaggcgaat accgcgaatt ccgtaagtgg agcgggagcc
    20521 aaatccacga atcgacagct gtaaatcttg cgcatagttc tgccggtttt gtacctgcaa
    20581 accaggcacg ccggtcagtg attcggacaa gttaatgcgc ggtgttgcca ggcgcatctc
    20641 ctcgccatcc accacgctta ctgctgcagg ggtatccagt tctgaaacca cctgcggcgc
    20701 ggcactgaca atcatggtct gttcatcagc ggcaaaaaca acgggggaaa ggacaagcag
    20761 ggcgggcaaa acggtctgtc gaacgaaaac aatcttcatg aaaaaagcca ggttaagaat
    20821 gggaaaacac cgtcatggta atgaaattgt aaatttatgg aaaacgaaac ggcacgatac
    20881 gttaattaat tgagagaatt gtagtcgtaa cggcaagaaa tgctccacat ttgagtaaat
    20941 aatgattacc attcccattt ataacaagag cgtaacgatg attacgctta gcgaagcatt
    21001 gtgaagcagc aaaaatatcg gttcatcaaa gggagtcgtc atgcatttac gtcatctgtt
    21061 ttcattgcgc ctgcgtggtt cattactgtt aggttcattg cttgttgctt catcattcag
    21121 tacgcaggcc gcagaagaaa tgctgcgtaa agcggtaggt aaaggtgcct acgaaatggc
    21181 ttatagccag caagaaaacg cgctgtggct cgccacttcg caaagccgca aactggataa
    21241 aggcggcgtg gtttatcgtc ttgatccggt cactctggaa gtgacgcagg cgatccataa
    21301 cgatctcaag ccgtttggtg ccaccatcaa taacacgact cagacgttgt ggtttggtaa
    21361 caccgtaaac agcgcggtca cggcgataga tgccaaaact ggcgaggtga aaggccgtct
    21421 ggtgctggat gatcgtaagc gcacggaaga ggtgcgcccg ctgcaaccgc gtgagctggt
    21481 agctgatgat gccacgaaca ccgtttacat cagtggtatt ggtaaagata gcgtgatttg
    21541 ggtcgttgat ggcgagaata tcaaactgaa aaccgccatc cagaacaccg gtaaaatgag
    21601 taccggtctg gcactggata gcaaaggcaa acgtctttac accactaacg ctgacggcga
    21661 attgattacc atcgacaccg ccgacaataa aatcctcagc cgtaaaaagc tgctggatga
    21721 cggcaaagag cacttcttta tcaacatcag ccttgatacc gccaggcagc gtgcatttat
    21781 caccgattct aaagcggcag aagtgttagt ggtcgatacc cgtaatggca atattctggc
    21841 gaaggttgcg gcaccggaat cactggctgt gctgtttaac ccagcgcgta atgaagccta
    21901 cgtaacgcat cgtcaggcag gtaaagtcag tgtgattgac gcgaaaagct ataaagtggt
    21961 gaaaacgttc gatacgccga ctcatccgaa cagcctggcg ctgtctgccg atggcaaaac
    22021 gctgtatgtc agtgtgaaac aaaaatccac taaacagcag gaagctaccc agccagacga
    22081 tgtgattcgt attgcgctgt aatagatatg gcgagggtga taacggagag acttgcctga
    22141 ctttgatcac gccgcatgcg gcacaccttc gtcagtaatc taaatttgcc cggcacgtag
    22201 ccgggcaatt ttgcaattag ctcgccgttt caggcttaaa cacaatttcc tgtttttctt
    22261 catcttcctc gacgactggc gcagtgctgt gaatttcagc aacgcgtttg cgcacaccaa
    22321 accagcctat aaccagcaga ataccgataa tcggcagcgc cgcgatagta taagtcccgt
    22381 tcgggtaatc gaacgccatc agcacaagga cactcagtaa aaacagtaat gtcagccagg
    22441 aagtgaaggg cgcgccaggc agtttaaaac tgacatccgc tgctttgcct tctttaatcg
    22501 ctttacgcag gcgcatctgg cacacgatga taaacgccca tgaagcgatg attcccagcg
    22561 acgcgaagtt caacacaatc tcaaatacgc gcgacggcac cagatagttg aggaatacgc
    22621 cgacgacata cacaaccagt gtcgccagaa tcccggcata cggcacatgc tgacgactca
    22681 ttttcgccat aaaactcggt gcggaaccgc ccatcgccat tgagcgcaga atacgtccgg
    22741 tgcagtacag acctgaattc aggctggaga gggcagcggt cagcaccaca atgttcataa
    22801 tgctgccgat atatggcaca cccagtttag agaaaaacgt cacgaacgga ctttgccccg
    22861 cctgatacgc gctccacggc aataacataa ccagcaacac cacggagccg acgtaaaaca
    22921 ggccaatacg ccaaatcaca ctgttaatcg ctttaggcac catggtctgc ggatctttac
    22981 attctccggc agctgtaccc accatttcaa tggaggcaaa agcaaacact acgccctgaa
    23041 tcaacaccag cgcaggcagc aaaccgtggg ggaagaagcc gccattatcg gtgattaaat
    23101 gaaagccagt ggtgttgcca tccagcggct gaccactacc gaggaacacc gtacccacga
    23161 ccaaaaaggt cacaatggcg agcactttaa taagcgcaaa ccagaactcc atctccgcaa
    23221 accatttcac accgatcata ttcatggtgc caacgatggt aagtgcagcg agcgcaaaga
    23281 cccactgcgg cacgccgcca aacgcacccc agtaatgcat atacagagcg atggcggtaa
    23341 tatcaacaat ccccgtcatc gcccagttga tgaagtacat ccagccagca acataagcgg
    23401 ctttctcacc caaaaactca cgggcataag agacaaaact gccactggaa gggcggtgta
    23461 gcaccagctc acccaatgca cgcagaataa aaaacgaaaa caagccacaa attaaataaa
    23521 ccagtgccag tgctggaccc gccatttgca gtcgggctcc tgcacctaaa aacaagccgg
    23581 tgccaatcgc gccgccaatg gcgatcatct gtacctggcg attgcccatc gctttgtgat
    23641 acccctcttc gtgggcatta agccagcggc gtttcgcggc gtgttgatct gaagtgtcgg
    23701 tgtcgtgttt actcattgct ctccctgatt gctttaatga aaaagtcata taagttgcca
    23761 tgaacaatgt ttattcatcc tggcgatgat agttatcacc attattcttt atgggtataa
    23821 aactatcggc gcagcatcct acccgttatc gataaacgat gcaaaacatc cccttacaat
    23881 cctgaagggg attaatacaa ctgacgaaaa aatgacaaat ccttttgctg gttaaccttt
    23941 gtactgtcct acatttaatc tttaaaagat tgtgaggggc atatgattaa ggtgtatggc
    24001 gtacccggat ggggctctac aatcagtgag ctgatgctaa cactggctga tattccttat
    24061 cagttcgttg atgtcagtgg ttttgaccac gagggaactt cacgcgagtt attgaaaacc
    24121 ctgaacccgt tgtgtcaggt tccgacactg gcactggaaa atgacgagat tatgacggag
    24181 acagcggcga ttgcattgat ggttctcgat cgtcgcccgg accttgcgcc accggttggg
    24241 cgtgccgaac gtcagcagtt tcaacggtta ttggtctggc tggttgctaa tgtctatcca
    24301 acgttcactt tcgccgatta ccctgagcgt tgggcatctg acgcacctga acagttaaag
    24361 aagaatgtta ttgaatatcg taaatcgctt tatgtctggc tgaattcaca attgaccgct
    24421 gaaccttatg cgtttggtga gcaactaacg ctggtggact gttatctttg cactatgcgc
    24481 acatgggggc ctgggcatga atggtttcag gataatgcca cgaatatcag tgcgattgct
    24541 gatgcagtat gtcagttacc aacgttacaa gaaatactaa aaaggaatga aattatttga
    24601 g
//
LOCUS       DAZFBR010000073        24467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFBR010000073 DAZFBR010000000
VERSION     DAZFBR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.073"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            758..4570
                     /locus_tag="ACHJZ2_003117"
     CDS             758..4570
                     /locus_tag="ACHJZ2_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFO4569189.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINARGSIENRDGNNFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRLIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNSL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGSLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4659..6278
                     /locus_tag="ACHJZ2_003118"
     CDS             4659..6278
                     /locus_tag="ACHJZ2_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO4569190.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTSVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGISEYLSAKATHTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQIENGYYNTAFFAQYAPRELTLQDKITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLVGSAVGIDGNIKSL
                     EYNFSVSTPLKYPATLDIDRVNVNFNFSYQM"
     gene            6294..6662
                     /gene="acpS"
                     /locus_tag="ACHJZ2_003119"
     CDS             6294..6662
                     /gene="acpS"
                     /locus_tag="ACHJZ2_003119"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO4569191.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTSHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIGQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            6676..7437
                     /locus_tag="ACHJZ2_003120"
     CDS             6676..7437
                     /locus_tag="ACHJZ2_003120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFO4569192.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIERQAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            7441..7989
                     /locus_tag="ACHJZ2_003121"
     CDS             7441..7989
                     /locus_tag="ACHJZ2_003121"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HFO4569193.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRETK"
     gene            8011..8292
                     /locus_tag="ACHJZ2_003122"
     CDS             8011..8292
                     /locus_tag="ACHJZ2_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4569194.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            8328..9488
                     /locus_tag="ACHJZ2_003123"
     CDS             8328..9488
                     /locus_tag="ACHJZ2_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HFO4569195.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSID
                     GYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            9562..12120
                     /locus_tag="ACHJZ2_003124"
     CDS             9562..12120
                     /locus_tag="ACHJZ2_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFO4569196.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVKDSERFA
                     KWFENARAAEVLHDIEYSDLNEDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSHARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSEATLPETESNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAVARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            12125..13348
                     /locus_tag="ACHJZ2_003125"
     CDS             12125..13348
                     /locus_tag="ACHJZ2_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFO4569197.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHASKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAIAMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMTNMQAGRAK"
     gene            13345..14298
                     /locus_tag="ACHJZ2_003126"
     CDS             13345..14298
                     /locus_tag="ACHJZ2_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4569198.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGSFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERQQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            14309..15016
                     /locus_tag="ACHJZ2_003127"
     CDS             14309..15016
                     /locus_tag="ACHJZ2_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4569199.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            15000..15656
                     /locus_tag="ACHJZ2_003128"
     CDS             15000..15656
                     /locus_tag="ACHJZ2_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569200.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVNQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            15657..16967
                     /locus_tag="ACHJZ2_003129"
     CDS             15657..16967
                     /locus_tag="ACHJZ2_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4569201.1"
                     /translation="MSGEILAVNCVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKMRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            16976..17764
                     /locus_tag="ACHJZ2_003130"
     CDS             16976..17764
                     /locus_tag="ACHJZ2_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HFO4569202.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLMGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            17761..19149
                     /locus_tag="ACHJZ2_003131"
     CDS             17761..19149
                     /locus_tag="ACHJZ2_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569203.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMSVDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSFSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHRETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLVDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            19163..20104
                     /locus_tag="ACHJZ2_003132"
     CDS             19163..20104
                     /locus_tag="ACHJZ2_003132"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO4569204.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMFNDFAGVDQQRVMHCRKAFSDAKVFK"
     gene            20101..21087
                     /gene="ycdU"
                     /locus_tag="ACHJZ2_003133"
     CDS             20101..21087
                     /gene="ycdU"
                     /locus_tag="ACHJZ2_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO4569205.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            21454..22656
                     /locus_tag="ACHJZ2_003134"
     CDS             21454..22656
                     /locus_tag="ACHJZ2_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4569206.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
     gene            complement(22843..>24467)
                     /locus_tag="ACHJZ2_003135"
     CDS             complement(22843..>24467)
                     /locus_tag="ACHJZ2_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569207.1"
                     /translation="FRRYEDLYVKLMHYSVFTDKTHQIIKQKYFNDIVPMILALDIRN
                     TYRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKDIF
                     DGVYEFIRNIRKNSTPGKTKLIATINTCKETCKHLLYLSNEDKEKIISDLDKVQVACY
                     YLTILLAFERRTSLTSTLATLYKMLISEREVSEYECQLLYLTNPIDVMNILNKYIYYF
                     PNENSPFYTLKIDSALSWDAIDAIRDYSISDIYLYPEQKTINCVVEIENIVFGGYIYT
                     LNNGVTLQNIENSLKDSSCHYVLNGYTEFVNCLRQLTSGKTESVHRTINKLNYEKLPF
                     GFIIAAFAILKIAFKIKFSKNHVNIRALLNDINYFMTYQGESINLISLDHEYPESCLQ
                     NDTNTYLLGRVIFLYNSMIYKFINCQEHETNNIHSAMINNLLQEVDIALGKINDIIDS
                     RNISAPHELANILTREKILTTREKKGNLISLFDGFTLFHCVGMITFLIHYLRTPEEKV
                     ENIFMLYGADKNNKLRRRLIYDALGIIQSQQE"
BASE COUNT         6467 a         5278 c         6233 g         6489 t
ORIGIN      
        1 ttacaatctt taatttagaa taacattttt atgaagacca gaatacgtta agggatgatg
       61 tatagcggtg attgctatta gatgggaatg aaagcttgcc aaaccgtttt gctcttcttt
      121 ctccctttca acgataagcc aggcataatg tggctgctta agccaattgt cgcaaagcca
      181 aagctctcta tatagctaac ctcatggtta atccgatctc agtaatacca tgagcaagaa
      241 ctctttcctt tttatataac tcttaagagt tacatgcgtg aatgcaaatg gtaatataat
      301 gaaatgtcgc ctgcccgtaa tgaccataaa gaatctaccc cgacgtaaaa gatatagaga
      361 ataagatgca gtaatgatga tgctttgtta atcaaataat aagatttaaa ttaattgtaa
      421 ctcaccaact ttgttgtttt attcggatgt ctgaaatgat gtcgtacaga aaactacata
      481 tcatttcaga cgtcctctaa attggatttt tttcacccaa ggagcaagga tgggggactt
      541 ttaaattatt gtgttttagt aagttaggtt cttcgccgtt cgatgaaaaa acaataactc
      601 ttagttgttt ttttgagtcc ttatttcagc ttatttttcg tgatttttta ttgcgccagg
      661 tgataggatt tgccgcaatc atttttgctg atattccgtg agtcatcaaa agtgatctgc
      721 atgtccaaaa tgaatagcat caataaggaa aacgttagtg gcgatgataa atttaagtaa
      781 ggaagcaacg gtggggaaag cattaacccc tattgctata cttatgatgt tgtcttttcc
      841 tgtagcttct caagcggcgg gattagtcat aaaaaatgga acggtatata acgccaatgg
      901 tgtgccagtc gttgacatca acaaacctaa cggtagcggt ttatctcata atatctggga
      961 taacctaaac gttgataaaa atggtgtcgt tttcaataat agcgctaatg aatccagtac
     1021 ttcacttgcc ggaaatattc agggaaacag taatctgacc tccgggtcgg cgaaggtgat
     1081 cctgaatgag gttacttcca aaaatccttc aaccattaat gggatgatgg aagttgcagg
     1141 ggataaagcg gatctgatta ttgccaaccc gaatggtatt actgtaaacg gtggcggttc
     1201 aatcaataca ggtaaactta ccttaaccac cgggacgccg gatatccagg atgacaagct
     1261 ggccggttac tccgtgaacg gcggtaccat tacgctcggt aaactggata acgccagccc
     1321 gacagaaatt ctgtcccgta acgtggtagt taacggcaaa gtgtctgccg atgagctgaa
     1381 cgttgttgct ggcaataact atgttaatgc cgcaggccag gtgaccggta gcgtatccgc
     1441 cacggggtcc cgtaacggtt acagcgtaga tgttgccaaa ctgggcggaa tgtatgcgaa
     1501 caaaatcagt ctggtcagca ccgagaaagg tgtgggggtt cgcaacctcg gcgttattgc
     1561 tgggggtgtt aatggtgtca gcatcgattc caaaggtaac ctgttaaaca gtaacgccca
     1621 gattcagtct gcaagcacga tcaacctgac aacaaatggt actctggata acaccaccgg
     1681 tacggtgaca tctgtaggca ctatctcgct taataccaac aagaatacta tcgtgaatac
     1741 ccgtgcgggt aacatctcta cgatgggcga tatctacgtt aacagcggta cgattgacaa
     1801 tactaacggc aagcttgcgg ctgcaggaat gctggcggtt gataccaata acgccacgct
     1861 gattaactct ggtaaaggga gttctgtcgg gattgaagcg gggctcgtgg cgctgaaaac
     1921 cggaacgctc aacaacagca atggtcagat tcgcggtggc tatgtgggtc ttgaatccgc
     1981 tgcgctgaat aacaacaacg gtgatatcca gaccaccggc gatatcgcca ttatcagtaa
     2041 cggtaatgtg gataacaaca aaggtctgat ccgttcgtcc accgggcata tcgttattgg
     2101 cgcggcaggt agcgtaaata atggttcaac caaaaccgcc gataccggca gttctgactc
     2161 tctgggcatt attgcagata ccggcgtaga aattggtgcg aacaacatca ataacaacgg
     2221 cggacagatt gcgtctaatg gcaacgtctc cctgtcaagt tacagcacga tcgacgacta
     2281 tgcgggcaaa attctgtcca acagcaaagt gattatcaag ggaagctctc tgcgtaacga
     2341 taccgggggg atcagcggta agcagggtat tgaagtcgcc gttggcggca gcctgaccaa
     2401 taatattggc gtgatcagct ctgaagaggg tgatatctcc ctgttagcca actccgtgga
     2461 taaccacggc ggcttcatga tggggcagaa catcacgatg gagtcgatgt ctggcgtcaa
     2521 taacaacaca gcgctgatcg tggccagcaa aaaactgaag ataaatgcgc gcggcagtat
     2581 cgaaaaccgc gatggcaata acttcggtaa tgcttatggt ctgtacttcg gcatgcctca
     2641 gcaaacgggt ggaatggtcg gcaaggaagg catcgagctt tccgggcaga acatctataa
     2701 caacaacagc cgtcttatcg ctgaggatgg tcctctgact ctgcaggcgc agaacacgtt
     2761 cgacaacacg cgtgctctgg tcaccagcgg ggcggatgca tctattcagg ttggcggaac
     2821 gtattacaac aactacgcta ccacctggag tgcgggcaac ctggatatcg acgcgaccac
     2881 gctgcaaaac agcagcagcg gtacgatgat cgataacaat gcgaccgggt tcatagcatc
     2941 tgataaaaac ctgtcactgg aagtggtgaa tagccttacc aactacggct ggatcagcgg
     3001 taaaggcgat gttgatgtca cggtgaataa cggcaacctg tataaccgca ataccattgc
     3061 ggctgaaaag gggctggata ttgccgcgtt gaacggtatt gaaaactgga aggatatttc
     3121 tgctggcggc gacctgacga tgaacaccaa tcgccatgtg accaacaact ccaacagcaa
     3181 tatggtgggg cagaatattg ttattaacgc ggttaacgat atcaacaacc gtggcaacat
     3241 tgtcagtgac gctgacctga acgtgacgac caaaggcaac ctgtataact atctctatat
     3301 ggtagggtat ggggatatcg cattgtcggc aaatagcgtg gcgaacaata acgcgaccat
     3361 cgaagcgaca ggcgatctga ttatcgattc gaagggtaac gtgggtaaca accgcggtaa
     3421 tctgcatgcg ttgaacggcg tgttgtctgt taaaggcaac aatctgaaca acgataacgg
     3481 tgaaattcgt ggttatggcg atgtcacgct ggcactgacg ggcaactacg acagctataa
     3541 gggttcgctg acctctgaaa cgggcgacgt gactctgacg gcgaacattg tagacaacgc
     3601 ctatggtttg attgccggtg agaatgtttc tgtcgatgct aaatcgacga tttacaacaa
     3661 cactgcgctg atcgcggcga ataaaaagct ggttattaac gctggcggca acctcgaaaa
     3721 ccgcgacggg aataacttcc tgcgtaataa cggcgcgctg tttggaatta ccgacaacgt
     3781 tggcggcatc gtaggtaaag aaggtgtcac gctttctgct cagaacgtct acaacaataa
     3841 cagcagcatc atcgctgaaa atggtccgct taatctgctg tccaggggaa cgctggataa
     3901 tacccgcgcg cttcttagca gtggggctga tgccatcatc cgtgcggcag ggacgttcta
     3961 caacaactat gccaccacgt acagcgccgg taatctcgac gtttatgcgg cgtcgttgaa
     4021 caacgccagc gatggtcgcc tggaagacaa taccgccacg ggcgtgattg cgtctgacaa
     4081 aaacctggat ctgagcgttg ataacagtgt cactaactat ggttggatca gcggtaaagg
     4141 agatgtgcat ttcaatgttc tgaaaggcac gctgtataac cgtaatgcca tcgcggcgga
     4201 caacgcgctg accattaatg ccctgaacgg tgttgagaac tttaaagaca ttgtggcggg
     4261 tactgcgctg actattgata cgcagaagta tgttaccaac aacagcaaca gtaatatgtt
     4321 gggacaaacc atcgcgatca atgccgtgaa tgacattaat aaccgtggaa atattgtggg
     4381 tgattattct ctgggtgtta aaaccaccgg taatatttat aactacctca atatgctgag
     4441 ttatggtgtc gctggcgtat cggcaaataa ggttacgaat agcggtaaag acgctgttct
     4501 cggtggcttc tacggtttag cgttagaagc aaacgaaact gataacaccg gtactattgt
     4561 cggcatgtaa gatgtaacca aagacagcag tggcttttaa tattaatgcg tcatgtcacg
     4621 caggggttgc tgctgtcttt atttagtttg agaagataat gcaattacga tttaattttg
     4681 gtttgctgct cattctttgt tgttttcagg ttgcagcgaa agacagtctt gataatttga
     4741 tcaagcagca aaaaaatgca gatgaaagca cgttgcgcgc gcataaatta gaaaaaaaag
     4801 atgtttactc aaatgcacaa cgtaatgcgt ttactctggg tgatctgccg caggaaaaga
     4861 attgctttat cattaatcgg attgaactgg aagataactt tctgggtggt cagttgattt
     4921 ctgaagtgca aaaagcgatt gccggacgtt gtgtcggtag tgctggtgtg caaaaaattg
     4981 cagctgcctt gcaggattat tatattaatg cgggatatat cacaacgcga attagtatcc
     5041 cttcgcagga tatttctaaa ggcaagctac gtttccaggt taatgccggg aagatagaaa
     5101 aaacgattgt cgaaggtaat gatatcagtg aatggatgtt accttttcag cgtaacgata
     5161 ttctcaatat tcgtgatatt gaacaggggc tggaaaatct tcagcgagta cctgacgttg
     5221 atgtgaaaat taatattgaa ccgggaacac gggatggtta cagcatcgtt catattgata
     5281 cacaccgggg gaaaaactgg agcgttcggg caagttacaa caattggggc gatgaagaaa
     5341 cgggccgtta ccagacaacc tcggtgggtt atctgtttaa tccagcgaaa atgggggatc
     5401 tgttttatct cgccggtacg cgtagtacta ccggacagta tgaaaacgtc agtagttatt
     5461 attccattcc ggtggggtac tgggagtacg gctttttcta tagcaaaagt aaatcgcagc
     5521 aggtgattcc gctttcgtac atctcgctgg attatgttgg catcagcgaa tacttgagcg
     5581 caaaagcgac gcataccgtt tatcgcgata aaacaaaaaa gttcgccggt agtgcagaac
     5641 tcattcgacg aaagtcgaat tacacgctta atggtgagga gctgacatta caggcgcggg
     5701 atatgggaaa tatcaagctg gggctaaatt ataagcaaca gttacccggc gcgttctggg
     5761 attcaaccct ctcgtggcaa cgctttttaa cctggttcgg tggtgaaaaa acgccggata
     5821 tgcaatatgg cgacgttagc ccggtaagca atatctttaa ttttgagggt aactataccc
     5881 gacaaataga gaatggatat tacaataccg ctttttttgc ccagtacgca ccgcgtgaac
     5941 tgactttgca agataaaatt acagttggcg atcgttggag tattcgcggg tttgaaaata
     6001 gcgtaggttt gtcagggaat gatggttttt atataaaaaa tacgctggca tttcctttgc
     6061 ctggaatgaa agctaattat tatgccggtc ttgattttgg gcaagtgtat caggacgctt
     6121 cttatggcga tgaatcactg gtgggctcgg cagttggtat tgatggaaac attaaaagtc
     6181 ttgagtataa cttttccgtt tctaccccat taaagtatcc ggcgacatta gatattgatc
     6241 gggtgaatgt aaattttaat ttttcgtacc aaatgtaaag gataacgaga ctcatgagaa
     6301 tagggacaga tattgttgaa attgccagaa taaccaaagc cattattaat ggagagatgg
     6361 ctttttatga gcgtgtttat acttctcatg agcgcttact cattaaccct gcagaacctg
     6421 attatgaacg ggcctctgga ttctgggcgg cgaaagaagc gttagttaaa gcgattggtt
     6481 gtggttttcg tgatggcgta cgtttccagg atattgaaat aaagcatgat gcagccaaag
     6541 caccttattt cgttatttcc ggaagactac aagaaatttt tatcgggcag gggttgcaaa
     6601 ataactccct gagtatttcg cattgccgta cccacgccat tgcgactgtc atcgtttatt
     6661 aactcaacat atgtgatgaa tatgtacaaa tataatgatt taaatgaaaa aaccgtactg
     6721 gtgacaggag ccagtggtga tattggactg ggtatttgcg aaaaatatct ggagcagaac
     6781 tgcgatgttt atgcgctcta taaatccaac gacgtgcaat taacggcgct gaaagcaagc
     6841 catcctgcag gcgataaact gcatattgtg cagtgcgatc ttgcctgccc ccagagcgtg
     6901 agcgcattgt gcgaacagat agagcgccaa gctggcaaga ttgatgtgct ggtaaataac
     6961 gcgggcatcg ttaaagacag cctgtttgcc tcgatgagtt atgaggattt cacccaggtt
     7021 atcgaaacca acatgttcag cattttccgt ctgacgaaag acgcgctgat gctgcttcgt
     7081 gcggcagaaa atcccgccat tattaacgtt gcctctattg cagcgctgat cccaagcgtc
     7141 ggtcaggcga attacagcgc gtcaaaaggg gcgatcctag gcttcacccg cacgctggca
     7201 gcagaaatgg caccctgggg cgtgcgcgtc aacgcggtcg caccgggaat gattgaatcg
     7261 aaaatggtga aaaaagtatc ccgcgcagtg gtccgtgcgg taaccagtac catcccgtta
     7321 cgccgtctgg gtaaatgtga agaagtggcg aacaccatcg tcttcttaag ttcctctgcc
     7381 tccagctaca ttgtcggcca gaccatcgtt atcgatggcg gcctggtcat gcggtgattt
     7441 atgtctaaat acacgattcc ttcgaaaatc ttcctcgaaa tgggcggctg gcgtcagccg
     7501 ctgctgatgg tcgataaaat tgcagattat aaatacggtg aaaatggctt tgtcagtgtc
     7561 gtgaaacacg tcacctataa cgaaccgtat ctactggggc atttcccgga agatccgatt
     7621 atgcccgggg tgattatttc ggaaatcttc ggccaggcca gcgagtatct ctctttcctg
     7681 accgacattt gcgatatctg gcgcgaacgt tttgaggaag agctgaagtc gttacgtgat
     7741 attcacgcac gcattcaccg ccctgaaatg ctggagatta tccgtacccg gcgcagccag
     7801 gtacgcggcg tgctggcggc tcagaacctg aaatttaaag atatcgccta tccgggtgat
     7861 tctattgatg ttgtcagcaa attggctttt tcggatgcca gtggctttaa acactattcc
     7921 gtgaccgcgt acgtcggtaa aaagctgatt agtcagggca ccattatcaa tttccgtgaa
     7981 accaagtaat ccaataagag agtaaatatt atgtctacca tgaatgacgc tattgaacag
     8041 cgtaaagaag ttctgatgac cattaaaact gagatcatcc agcgactgaa tattcaacgc
     8101 gatgaagcgc aaattgatga tgatactgcg ctgtttggta atggttttaa actggactcc
     8161 gtcgatgcaa cggaaattgt cgttctgctg gataaagtat ttgatatcaa gatcagcgaa
     8221 agtgacgacc cgtcctatat gcgcagtatc aatagtctgg ccaccttcgt tattcaaaag
     8281 aaaaacgatt aactacttat taaagaatta attatcagga cacgtttatg aaatctcttg
     8341 ctgaatttca cttgaaaaat aatgcggtaa tgggggtata taacaaccgt accctacctt
     8401 cttcttatca cgatgctatg accgaatata aagccgttcg tgaaaacgcg ctgctggtgg
     8461 actattctca cctctctatt gttagcgtca tgggcgatga tgcctgggcg ctgatcaacc
     8521 agctggtttc cgcagatgtc tccatcattc gtgatgagca ggcaatctac tcactggtgt
     8581 tgaatgaaga gggcaccatt cgcggagacg tctatgtgct gtgcagcatt gacggttatt
     8641 acctgctttc agaagatatt tcggctgccg aattgatcgc cagcatgaat accattctcg
     8701 aaaaagcgga agaactggat attcaatcga tgcctgaaat tcaggatatg cgggaaaaca
     8761 actggggggc aatcctgctc gaaggcccat actcctggga gatcatgtct gaaattcatg
     8821 gctttgacgt gattggcctg ccttactacg aatacatgaa taccgaggag gatctgctgc
     8881 tcttccgctg tggtaaacac ggtgagtatg cgtacatgac catcggtgag caggcgaaac
     8941 tggcggagca gtgggaaaaa ctgttaaccg ttggcgagaa atacctgatg cagaccggcg
     9001 gcctggatta tcaaaaaatc gtacgcctcg aaaatccgtg ctgggatgcc agcctctggg
     9061 aaggtcaggc ggtgaatccg gttcagttgc agatgcagtg ggcggtgcag tacgacaaag
     9121 atgattttat tggcaaggac gcggtgacag agctatccca ggagtatacc ggtaataaac
     9181 tcatcggcat gatcgcacag gaagaatgcg aaggcattga ggctggcgat cgcgtgctgg
     9241 tggaaggtca agacgtgggc tatgtcgtga aggcactctt ttccccagcg ttgcagcgtt
     9301 tcattgccct gaccctgctg gaaaaggatt acgcctggtc tgacatcagc ggctacgaaa
     9361 ttcaaactgc tcacggaatt attccggcgc aatcaaaatg tatgccgttt atttataacc
     9421 tgagcatgtt ggttagccca actgagcaca gttatatcga cgcgtcaaaa aataaaagcg
     9481 cagcgtaatg aatatcgccg cgtgactata gcttacgcgg cttaaattaa ccccaattgg
     9541 cgggattgcc aggtattatt catgaaaacg aacaggaaaa gagttgttat taccgggctg
     9601 gggattttgt cctcactggc ggaaaatatt caggatttcc gggaagcatt attaaataaa
     9661 aaaaatggcg ttaaggatag cgaacgcttt gcaaaatggt ttgaaaatgc gcgtgcggca
     9721 gaagtgttgc atgacattga atactccgat ctgaacgaag atgttatcga ttcactggat
     9781 aacgcagcgc tctgggccta caaagtgggt aaagatgcgc tgagtcaggc cggcctgatg
     9841 gatagccgtg acaccctcaa agaaacaggc atgatcgtcg gtgtttcgtc cgcgggaaca
     9901 gaggcatttt tgccgctgtt cgaacagcgc atggaagatt tctcgctgcg taaagcactc
     9961 ttttccggcg cattctcatc ctgttgttcc agcgtttcaa cgctgctggg cttacagggt
    10021 ggggttgaac tggtggcgac cgcgtgtacc gccagcccga acgccgtggg gatggccttt
    10081 gactacatcc agaatggtaa aagcaaaacc atgctggcag tgggcactga gcccatctat
    10141 ctgccgacct ttgccggatt ctacgcactg aatgtcatgc atccagacac ttgtacgccg
    10201 ttctcgggtc agtccggtat gtcgattggt gaaggcgctg gcgctatcgt gctggaagag
    10261 tatgagcacg ccgtagcacg cggggcgacc atttatggtg agatcctctc ttacgccact
    10321 tcctgcgatg ctttccatga aaccgggcca gatccgcgtg ccagcggagc ggtgcaggtc
    10381 atgaacaagg caatggcaaa tgcgggcgta accccggagc aaatcgatta cgtcaacgcg
    10441 cacggtacgg gtaccgaagc caatgaccgt attgaaacgc tggcaatgaa aaaggtcttc
    10501 gctaaccacg ataaactgct ggtgagctcc accaaatctt actttggcca caacattggc
    10561 gcagcgggga ttgtggaact gattgcctgt ctggcaacgc ttccggataa ccgcgtactg
    10621 ccgacgctga atttcagcca tgcacgcccg aattgcgatc tggactatgt gccgaatgag
    10681 tttcgcgatc gcaaagtgaa catcttcatg aagaataact atgccttcgg cggcaacaac
    10741 tgctgcatga tcctgagcat ggagcccgcg tccattccgg tcagtaccta tgattcgaag
    10801 cgtgtggcta tctcgggcgt cggtgcagta tccgcaatag gccacacgct caaccaggtt
    10861 ctggaaaaca tctgggcgca ggaccaccac gttgaactgg gtagcgtaac cttcccggac
    10921 gatacactgg aagaagccaa agagctgctg gccgtgctgg atgaatcaaa ccagtttgcc
    10981 gagctgtttg atgaagagta cagcttcagc gaagctacgc ttccggaaac ggaaagcaac
    11041 tttaaaacgt tccaggtttc cgggctggag ccgcgtaaac acctgcgtcg tttcgatcag
    11101 cgtaaagcga cccgtggcgg cacgtttgcc ctgatcgcac tcaccgaagc gctggaacag
    11161 gcgaagcgta aaatcaaacg cgacggcgat gagttgggca tgatcatggg gatgtcccgc
    11221 ggcccgcagg aaaccaccta caaatatctg caaagtctga aacccgatcc acgcaaagta
    11281 cgtacatcgg aatttcctgg ctcgctgatg aatgcgatcc caacgttctg cgggatctct
    11341 gaagggatca aagggtatac cactacgctt gcgacagggg aaaactcggc gctgggcgcg
    11401 ctgacctacg gttatgaaat cgtccggcag aacctgcaac cgcaggtatt ggttggcggc
    11461 gcggatgagt acttcccgtc gatgtcgctg tatatggatg cggtgacaca aaagatccac
    11521 atgacggctg aggcgagcga ctatcagatt tacggtaaag atccgcaagg ttatgttccg
    11581 ggcgaagggg cttgcatgct gctgctggaa gatccgcagt tggctgtggc gcgtggcgct
    11641 gaggttctcg cggaagtggt gggctacggc aagtcatgca gcaataccta cttcgacgcc
    11701 tcattgttgg aagaaaaatc cgcgtcgatg gcgctggcca ttgaacgcgc ccttacgcat
    11761 gcgggcatct ctgctggcga tattgatttg gtatgtggta ccagcaacgg tagcgatgac
    11821 cattctcgta tcgaaattga cgcgatttac aacaccttcg ccgcgcaaaa cccggcggtg
    11881 ccggtagtga actataacgc ttgctttggt tttgtcgcct ctgccgctgg tttgcttaac
    11941 ctcgccgtga ttactgactg cattaagaag caggcagtac ctgcgattcc aaatacggtg
    12001 gaattctttg atgagcgtgt gaattttgta cgccagtcga tgaaacttgc gctgaaccat
    12061 gtcctgcttg ttggcgcaac ggaaggcggc aactactacg cgttcgtcat taagggataa
    12121 gactgtggac aatgccgcta ttatttcggg ttacagcgtg tggatgccgt atgccgaaga
    12181 ctgttcgcag ttaattgaca atctgaaaca ggggaaacgc gttgcgcgta caccctggtt
    12241 tacctccaac gaggaagcca ttaaatgcgg gtttaagggt aatccgtcag tggcgacgtt
    12301 gaaacaggtg gacgatagcg cattggacct gctgtctcag ttgatcgacg aagccctgga
    12361 acaggcaatg ctggataagc attgcctggc cgggcgcaac gttcgcgttt atctgaccgg
    12421 tattgggccg cgtatcgacg ggctggatta caaatctttc tataattaca acgatgtaga
    12481 agatatcaac ttaacgcaat ccatcacaaa tctgcatgcc tcaaagatgt cgcaggacac
    12541 gatttcaagc catcttgcgc gcaaatatcg cttgcagtat ttgccgccta acatgaactg
    12601 caccagtaac tcgtcactga ctgcggtgca tctggcgacg cagggcattg agcagggcgg
    12661 gattgatctc gcgattgtgc tgaactgttc gaaaattaaa acccaggaca tctggttcct
    12721 ggaaacgcaa tcgatgctgg acagcgaaca agtgcagccg tttggtgaaa acagtaaagg
    12781 tgtggcgttc gcagaaggtt ttagcgcgct cctactggaa agcgctcatc accgccgggc
    12841 gcgtcaacaa agtgagggcg taagggtgca aacgacttac acccaaatca gcgcgggccg
    12901 cagcaacgat gcctcctggc ttagcactaa cgtgcacaag gtcatgcagg ccgcgatgaa
    12961 gcaggctgaa attgcgctgg atgatcttgc agccattctt ccacacggca acggttcggc
    13021 agtgagcgat aacgcggagg ccaaagccat cgccatgttc gcaggggagc gacagatccc
    13081 cgttctcgcc tataaagggc agatcggcta taccgcaacc ggatctggtg ttgtcgatct
    13141 gatcattggc caccattcgt tgacgcatca tcaactgatc gcacctgttg gcaacgacgt
    13201 cattatcgac agcatggctt cgctggtact tacggacggc agcgtgacaa accatagcaa
    13261 acgccatttg ctgaaggttg gtgtgggtgt cgatggttca gttattggcg ttgtcatgac
    13321 aaatatgcaa gcggggcgcg cgaaatgacg gaaaagagta tctacctgtc ttcctgggcg
    13381 cttacggaac cggacgacat tttttatttt tatcagccga cctggctcaa cgcctgggaa
    13441 gcacatatgt acgaaagggg attatacggc acccgtgcta atggctgggg actgaagcgc
    13501 tttggcgtgg agaacgatat ttatgcacca gaacggatcc gtaatccaca atatatgcct
    13561 taccttgagg cgatggagca ggtgaccgat tctgcattac aacaaatgcc gactaaagaa
    13621 cgcatgaagt taatgaaatc gcgcacggca tttatttatg ccgattcctg gggcgaatcc
    13681 ggttcgtttg aaaaaatttc cagtgcgctg catatcgcca ctattgatac gcttcccaag
    13741 aatttactta agaagttttc gataacggaa ccgacttgca agattcgcgg cgaaaaacag
    13801 gcattcgttc aggcgctgcg tatggcgcag gattatctcg cctgggatgt attcgataac
    13861 gtggtgattt gtgctgccta tcgggcaatt ccggtgctgg tattctctga agaagatgtg
    13921 ggtaaaccgc ctaagcgttt atttccccgt cgtgctgatg aggtgaattt atctgttgaa
    13981 cgagtgggtt gttttattct cagccagcgt gagagcgcca tcaggctgca atgcggggaa
    14041 tatcattccg gcactgcgcc acagcatgct acggatattg atgtcatggc cttcagcgaa
    14101 cggcaacaaa tgccgtcccg gcctgctgct ggcgttatcg acctggtggc ccattacggt
    14161 gaaagcggtt gtctgacgcc cgcgttgagt atggattatt taacccgtca tattcagccc
    14221 ggtggaaaaa tgcgtacggt cattgccgat aaacagtttg gttatcacta ctttgacctt
    14281 gaataccttg aaggataacg acagtcgtat gcaatctact attaaatcgt tgcccgctat
    14341 ttcgcttagc aatatttata agtgttatgg cgctggggca ggaaaggttg atgcgcttaa
    14401 aagcattaat cttgatatca ataaaggcga atttctcgca ctgtgtgggc cgtcaggcag
    14461 tggtaaaagt actctgttga atattctctc gggtattgat aaaccgacca gcggaacggt
    14521 gatattttta aataaattac tgaaccaact gccagaagag cagttggctg tgattcgtgg
    14581 aaaacatttg ggttttattt ttcagttttt taaccttatg ccggtattaa atgttttcga
    14641 taacgtttat ttcccactgg tgcttaatgg tcagttcagt aaaaaagaag ccactgaacg
    14701 gacgctgcat tatctcgata gtgttggatt aagcggtttt ggcgatcgta aacctgggca
    14761 actatcgggc ggtcaacaac agcgtgtcgc catcgccagg gcactggccc acgaaccgat
    14821 ggtggtcatt gccgatgaac cgacgggaaa tttagacctg gcaaccggcg aggctatttt
    14881 ggacctgttg ttgacgatta atcagcagac cggcaccaca tttgttattt ccacgcactc
    14941 cagcgaatta aaagcgcggg cacggcgagt ggttgaaatt caggatggag tattgattca
    15001 tgactcagat gcataaagga tttttgctca tcggcgtctt tttgtacgcg gcgtttgtgg
    15061 ctccgaagat ttacgcggca acacccgacg ctgttaacca ggttctgcaa caggggctcc
    15121 cggcgcagcc tgccgcgctg gcgacgctga ccgaggcgtt aaagaaagag tatgaagcca
    15181 ggcacaatgt ggtggcgctg gttttttacg cctatggctt actgcgtcaa gcggacggtt
    15241 atgcggcaac aaacgatttt atccatgcct cagaatacgc caaaagcggc tttttctggc
    15301 tggatgaagc ggtggatttg caggagaaaa atcaacgcgt acggtattta cgcgcccgcg
    15361 tagatgccta tttacccgca gactcagggc gttgcgtggt gacagtcaaa gacaccgaac
    15421 acatgctggc tgacccggcg atatggacga caacgatccg cgaccatatt ctggcgatgc
    15481 gttatcgcgc attacgtcac tgcaaggaca ccacccgcgc gaatgcgttg ttggcgcaaa
    15541 ttaaagggca aaacgccgcg cttgcgcagt cgctgacgca ggattttaat gttgtaccag
    15601 aatgggatag tgaagagtta acccaggtac tgctgccgct gatgaaaggt gagtaaatgt
    15661 ccggggaaat tttagctgtt aattgcgttc tgctgctgat tctgtttttt gtgttttatc
    15721 agcttctgtt ttattcacga gatgtcaaat ttgcattttt gaatctcttt cgccacaagc
    15781 gtcgctcttt ttctaccata accgctattg tattgggtgg cgtggcaatt tttttatatg
    15841 gcggatttat tgattactct ttctggatcc tgaaagagca gacgattcgt accaatattg
    15901 gtcatgtaca aatttacaat cagcactatt ttgaaacgtc gaataaaaat aagagcctga
    15961 ttgccgatta tgcggcgtta aagaaagcta ttttgtcgga cccgacgctg gcggcggata
    16021 tttccacgct ctccgggcaa ctggagttta ctggcgtaat ttcacattac gagagcgaaa
    16081 cctccagcta tttctccgcg ctgggggtcg agccgttacc cgctttaaag ctgggttcgt
    16141 tcgataagat tatcgcgggg agcgatcttt cccgggtaaa aggtgatgaa atcaccctgg
    16201 gcagcggtct ggcgaaaacg ttgaatgcga aatatgacga ctggctggat gtgatggtgg
    16261 tcaacacggc gggcgggcag ggcgcattgt cgctaaaaat gcgtggcatt tttgagtcag
    16321 gcattaaaga ttatgacgat gtcgcgatga agatcccgct ggacaccgcg cagcgcatga
    16381 tgggtacgga tggcgtcagc aaagtgctta ttctgcttaa agaggatgac accgcggcgt
    16441 ttagcgcaaa gctgcgtcaa tttatcgaac gcaatcattt gccgctggtg gtcaaagact
    16501 ggcgtgatgt ttcactcttt tatcaacagg ttgaagggtt gctatcgggg atctatttct
    16561 tcatcaagct gattgttgcg ctgattgtca tcttcatgat cggcaactcg atggcaatga
    16621 acattgttga gcgcacccgt gaaattacca ctctgcgtgc cataggcctg aagccgcttc
    16681 acgtcacgcg cttgtttctg acagaaggca tttttattgg tgtgattggc gcagtcggca
    16741 gcctgctggt ggggggcgtg ttagcctgga ttattaatct ttatggtatt gcgatgccgc
    16801 catcgccggg acaaaccatc ggctataccg cctttataaa aaccagtaac ccggaattga
    16861 tttgggtgac ggtggtatta cctattttga cagcaacagg tgcctctgtg ttaccggctt
    16921 tacgtgcttc ccggctgaat atttctgacg catttaaatt ctcatagggc taattatgaa
    16981 tatggctttt tacggcaagt ggtttgcttg cctgtggctc gctacgtcgt gcgttcaggc
    17041 ggcgagcaca gataataagg cgctggagat aattcgtcgc gctgacgaaa ttcgttcgcc
    17101 gaataaaccg tttcgttaca cgctgacggt aacggaatat aaagcgggcg caacccagcc
    17161 agagaataaa caggtcctcg atatttccat gcgctttatg aaaccgcagg gtaatgaaaa
    17221 agcggatgca cgctcgctgg tgcgttttat ttatccgccg cgagataaag gaaaaatcat
    17281 gctttccgac tggtacgatt tatggtttta cacgccggaa ctgcgacgtc ctatgcctat
    17341 ttcacggcaa caaagattaa ttgggcaaat ctctaatggc gatgtcattg tcactaactt
    17401 tgaatatgcc tatgactcga ccctgatggg ggaagtcacg tgtgctgaaa agcagtgtta
    17461 caagctggcg ctggtgcgta aatcggcgga tatcacctgg ccgaaggtta tctattacgt
    17521 tgaaaaagac ggtgataatc gcccgtggaa ggcggcctat tattcgcagg atgaccagtt
    17581 aataaaagaa gtgttgtatc aggatttcca gccggtgttg gggaaaacgc gacccatgaa
    17641 aataaccgtt accgatgttc gtcacggcaa taactattca gtgatggagt acagcgatgt
    17701 tcgtctggaa tcgctgccgg agtttcattt caccaaagag tatattcagc gaggtgcgaa
    17761 atgaaacgta cgctgaagat ctcttcgttg ctgtgtgttg cgctgccgtt gacggtacag
    17821 gcggattgcc tgtcaggtga cgaggtggca caaaatagcg atgtgacgct ttacgagcag
    17881 gtgtcttatg tcaataaaca ggcttcggcc tggcagattg caggcaaaaa tccctacacc
    17941 cgtcacaacg gttatcagga ggcggggatt ggcatcaata gcggttgttc gataatcgac
    18001 aatacgctgg accttaagct caatctgtat ggaataaacg aatatgcgct taaacccgca
    18061 gggaaattcg aaacggatga tagccgcacg cgtgcgttaa ttaaccgcct cagtctggtc
    18121 tatagcgcct cggacagcgt tcaatttgaa gcaggcaaat ttgccgcgcc gtcaggaatg
    18181 ttctttttgc gttcgccttc tgatttgcaa acgcattatt acaccggatt ccagtcgacg
    18241 cgtttgcacg atcccaaaat gacatcggcg tatcaggcgt catcgtgggg cgcaaaaatg
    18301 agcgtggata cacgcgatta tgcgttttcg gcgagtgtca taccgaagtt agccaccatc
    18361 gacaaacgct atgtgacgtc tggcaactgg tcggctaatc agcagggaaa cagcgacgaa
    18421 gcctatctgc tgagcttcag cgatcatcgc tttggtgagc acacgccgac agtcaatgtg
    18481 cggctcggac catcgccgtc actcgcgctg tctgacagtt atcactacac cccgcagctt
    18541 acgctgaatg tcgacgcagc ctaccaccgc tcccagcaat ggcggcatct ttcccaccgt
    18601 gagacggcgc aggtcgagga atatcagttt ccggattcgc tctatgaaac gaaagatgaa
    18661 agcggcgtag agctggcgct gggtgggcag tacaccagtg ataatttcag cgtgtttggc
    18721 gttgagtatt actttcagag cgagggttat tcgcgcgctg aacagcgtca gcagcgcgag
    18781 ttaattgact tcctcaatac cacaaccggc tatgcgccac tggatcaggc atttgattct
    18841 tacaaatacc tgatggcttc agaaatcagc aataccgcca atcaggggat gctgcagggt
    18901 aagcactatc tgaacgcctg ggccagtctg ccgctggcgg gagagtcgac gctgcagcct
    18961 tcgctggtgg tgaatctcgt tgatggcagc acgttgctgg gcctgcatta ttccacgccg
    19021 ctcagcgcta taagtaacca gcttgaagcc tatgccggtg gctatagcgc gctgggaagt
    19081 cggtactccg aatttgcgct ttttggcgac acgttaggcc tttatttggg ctttaaatac
    19141 tatctataag gataaagaga tgatgaataa tgcttcacaa ccgacagtac tgctttttgc
    19201 cggtcagggt aatccggtta ttggtatggg cagcgatctt tgggatctca acgccagtac
    19261 aaaacatatc tgggattgtg ccagcgatat ttccggactg aacctgcgtc gcctgtgtca
    19321 gaaagggccg atgaatcgcc ttattcaaac gacggtacaa caactggcgg tgaccgcaat
    19381 taatgtaacg ctctataccc tgtgccgcga gcgtcttgag aatttaacgg ttgtgggctc
    19441 ctgcggacac agcgttggtg aatacagtgc gctctacgcc gctggagcta tttctcttga
    19501 gacgctattt ggcatgattc acttcagagc cagcctgatg catgacgaaa gccagagaaa
    19561 taaaggcgca atgctggcgg ttaaaggcat caaccgcgaa caactgcaga cgatgattac
    19621 ggaatcaggc attgcgctgg atattagctg tgataacacg ccgcgccagc aggttattgg
    19681 gggaacgcag gctgcgttga atgaatttgc cacgctgctg atggccgctg gatacgaacc
    19741 ggttaaactc ggtgtgagcg gtgcctggca cacgcgtttg atggaagacg gcgttcaggc
    19801 aatgcgcgat tatcttgccg gactggatat cgcttcgcca gagcatcagg ttctgatgaa
    19861 cgtaacagca aaaagcgagg tcgctccgtc aattatcaaa gaaaatctct cattacactt
    19921 aactcatacg gtgaaatgga ctgaatctct cgatacattt ctgaatatgc caacctcggt
    19981 agctttcctt gaaataagta ataagcccta tttggggaat atgttcaacg atttcgctgg
    20041 cgtggatcag caacgagtta tgcattgtcg taaagcattt agtgatgcga aggtattcaa
    20101 atgattcctg attatttaac ttttattcgc tttcaggata aacgaagtct gatatacatt
    20161 tatgctattg gacttattct gataggcttt tattggaaaa atgcaggatt tacttttcca
    20221 tcagaggata ttggtgtcgt tagtgggatt ctggccctgg tgctgtataa ttttattttt
    20281 gatctcaagg cgtactgggc ttataaatgc gtcacgaaga atatcgattt ttcgtggttt
    20341 aagaaaaagc agaaccacaa aatagaatta tttcttacgc aacctctggt ggcaggattt
    20401 ctgtcgttaa tcatgttgag tgcaatgagt tgggggctat accagcgtct accctcgtta
    20461 tatgcgctgt tcctgatttc gttacttggg ccgttggtta tctttctgct gtttcggatg
    20521 atccgcacca gttatgtcaa gcaggtcgct atttcagtag cgaaaaaagt aaaatataaa
    20581 agtctgactc gctatgtgct gctttcggtg tgcatctcaa cggttgttaa cctgcttact
    20641 atcagcccgt tgcgtaacag tgattctttt gtgatagagg ggcagtggtt aacgtttaaa
    20701 tcgataattg cattgctcat tctttgtggc gtagtgttgg cgattaatct gttttttctg
    20761 cgcttctcca ggcggtacgc ttttttgggc aggctttttt tgcaggaaat cgatctcttt
    20821 ttctccagtg aaaatgcatt gtcgaccttt tttgccaagc cgctttggct tcggttattc
    20881 atattgctgg ttattgaagt gatgtggatt acgctggtgt cggtattggc aacgcttgtg
    20941 gaatggcgga tttggtttga agcctatttt ttactctgct atgtaccgtg cttaatttac
    21001 tattttttct attgtcgatt cctctggcat aacgatttta tgatggcatg tgacatgtat
    21061 ttccgttggg ggcattttaa taagtgagga agtgatagga agtgaccaga taatacatat
    21121 atgttctgta ctctcttgcg cattttgatt gttgactgag caaccagaca gttgatgtgc
    21181 acgatttccc ctcgcccaaa cagacgtggg cgggggcacc cggtgtgtgc aatatacgaa
    21241 aaaaaagccc gtactttcgt acgagctctt ctttaaatat ggcggtgagg gggaccgcct
    21301 gaaataaatc tgacttaatc caggggtgtt atataatttc attataaaca atgtattaca
    21361 ctttttgttg tccgatatta tctaatgtat tcttagtgaa gcgagagact aatggcatac
    21421 tggatggcat accagagtga cgggaattag cgtatggcag tattgacgga tacgaaagca
    21481 agacatatca aacctgatga caaaccattg ccccatgggg gaattaccgg actgaccctt
    21541 catccttctt cagtaaaggg gcgggggaaa tgggtttttc gttatgtaag tccggtgaca
    21601 caaaaaagac gtaatgctgg attgggaact tacccagagg tcagtattgc tgaagctgca
    21661 cgtactgccc ggataatgcg agagcaactt gctgcaggtg atgatcctct ggagattaaa
    21721 aaggctgaat ctgagaaagt cgctatccca acatttgccg atgcagccag gcgtgtacat
    21781 gcagaactgt ctcctggatg ggaaaatcca aagcatgtaa ggcagtggtt atcgacgctt
    21841 gagaattacg cgtttcctca actgggagca aaaacgctgg attcgattac ggctgcggac
    21901 gtggcagaaa cactgcgtcc agtctggtta accttgtcag aaacggcaag ccgggttaaa
    21961 cagcgcattc atgttgttat gcagtgggga tgggcgcacg gtttttgtgt agcaaatcct
    22021 gttgatgtgg ttgaccattt gcttcctcag cagacaagag gacgtgatga acaccaaccc
    22081 gcaatgccct ggaggcagtt accgcttttt gtggcgacca gtgtgtatac cgatgaacct
    22141 tataatgtta cccgcgcact gttattaatg gtgatactta cagcaactcg ctcgggcgaa
    22201 gccaggggaa tgcgctgggc tgaaattgat tttcataagc gggtatggac tatacctgca
    22261 gaaagaatga aagccaggat acagcatcgt gttcctttat cccggcaggc tatttacatt
    22321 ctggaaaata tacgtggcct gcatgatgaa ctggtgttcc cttcacccag aaagcagcag
    22381 atcctttccg atatggtgtt gacaagtttt ctgcgtaaaa agaaagccgt cagtgacatt
    22441 ccggggcgag ttgccacggc acatggtttt cgctcaacat tcagggactg gtgtagcgaa
    22501 caggggtatt cgcgggatct ggcggaaagg gcgctcgctc atacgctgaa aaataaggtt
    22561 gaggcggcat atcatcgtac tgatctactg gagcagcgtg taccgatgat gcaggcatgg
    22621 gcggattatg tgatgtctca aattgtgaat aaataatagt gaaaatgtat attgtacgat
    22681 catgatttga tatgtgatcg tagcagtagt aattaagaaa ccgcttttat gtcaaatgta
    22741 ttgagctgat aattatcaga tcgtcaggaa agcgtatttt gattaacagg ggattactta
    22801 acaatcccct ttttataaaa aataatttgc ctgtttaact cttcactcct gctgagactg
    22861 aattattcct agtgcgtcat aaatcagtct tctgcgtagt ttattgtttt tatctgcacc
    22921 atataacata aatatatttt caactttttc ttcaggtgtt ctgagataat ggataagaaa
    22981 ggttatcatt ccaacacaat ggaataaagt gaatccatca aacaggctta tcaggtttcc
    23041 ttttttttcc cgtgttgtaa gtattttttc gcgggtaaga atatttgcca gttcatgggg
    23101 ggctgatatg tttctgctgt ctataatgtc atttatttta ccaagggcta tatcaacttc
    23161 ctgtaatagg ttatttatca tagctgagtg aatgttattg gtttcatgtt cctgacagtt
    23221 tatgaactta taaatcattg agttatacag aaatattact cttcctaata aatatgtgtt
    23281 tgtgtcattt tgaagacagg actctgggta ttcgtgatcc agtgaaataa ggttaatgga
    23341 ctcgccctga taagtcataa aataattgat gtcatttaat aatgctcgga tatttacatg
    23401 atttttactg aattttattt taaatgctat ctttagtata gcaaacgcgg caatgataaa
    23461 tccaaaaggt aatttctcat agttcagttt attgatggtg cgatgaacac tttcagtttt
    23521 tcctgaagta agttgtctca aacagttaac aaattctgta tagccattta agacataatg
    23581 gcatgaagaa tcttttaaag agttttctat gttttgtaat gtgacgccgt tgttcaatgt
    23641 ataaatgtaa ccgccaaaga caatgttttc aatctcaacg acacaattta ttgttttttg
    23701 ttcaggataa agataaatat cagaaatact atagtctcgt attgcgtcaa tggcatccca
    23761 cgataatgca ctgtcaattt tcagtgtata aaatggtgag ttctcattag gaaagtaata
    23821 tatgtatttg ttcagtatat tcattacatc tattgggttg gttaaatata ataactggca
    23881 ttcatattct gaaacttctc tttcgcttat cagcatttta tataaagttg ccagggtgct
    23941 tgttaatgaa gttcgtcttt cgaaagccag taatatagtg agataataac atgcaacctg
    24001 aactttatct aagtcagaaa ttattttttc cttgtcttca ttacttaaat aaagcagatg
    24061 tttacaggtt tctttgcatg tgttgatagt tgcgataagt tttgtttttc ctggtgtact
    24121 gtttttgcga atattacgaa tgaattcata tacgccatca aagatatctt tgaaatgcgc
    24181 attcgctggc gtgtatccgg ataaacataa cttaacgtaa tttcgcagat atgtccttgc
    24241 agcatgatat atatcatcct cattatccgg tatctgagtg agaaaagaat gaatatgata
    24301 gtaaaatgcc atctcattat ccgggcgata tgtgttcctg atgtcgagtg cgagaatcat
    24361 tggtacaatg tcattaaaat atttttgttt tattatttga tgtgttttgt ctgtaaaaac
    24421 agaatagtgc attagcttaa catagagatc ttcatatctc ctaaata
//
LOCUS       DAZFBR010000074        24076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFBR010000074 DAZFBR010000000
VERSION     DAZFBR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.074"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..180)
                     /locus_tag="ACHJZ2_003136"
     CDS             complement(<1..180)
                     /locus_tag="ACHJZ2_003136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569208.1"
                     /translation="MWISSKVSRAKAPKNGRLPKKAAIIHKIPEFVGGTADFRRRSFS
                     FERKRKKKIIFLPPFI"
     gene            179..514
                     /gene="yjdM"
                     /locus_tag="ACHJZ2_003137"
     CDS             179..514
                     /gene="yjdM"
                     /locus_tag="ACHJZ2_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO4569209.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            773..1216
                     /gene="yjdN"
                     /locus_tag="ACHJZ2_003138"
     CDS             773..1216
                     /gene="yjdN"
                     /locus_tag="ACHJZ2_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO4569210.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1349..2137
                     /gene="phnC"
                     /locus_tag="ACHJZ2_003139"
     CDS             1349..2137
                     /gene="phnC"
                     /locus_tag="ACHJZ2_003139"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO4569211.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2162..3178
                     /gene="phnD"
                     /locus_tag="ACHJZ2_003140"
     CDS             2162..3178
                     /gene="phnD"
                     /locus_tag="ACHJZ2_003140"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4569212.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            3284..4063
                     /gene="phnE"
                     /locus_tag="ACHJZ2_003141"
     CDS             3284..4063
                     /gene="phnE"
                     /locus_tag="ACHJZ2_003141"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO4569213.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4084..4809
                     /gene="phnF"
                     /locus_tag="ACHJZ2_003142"
     CDS             4084..4809
                     /gene="phnF"
                     /locus_tag="ACHJZ2_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO4569214.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4810..5262
                     /gene="phnG"
                     /locus_tag="ACHJZ2_003143"
     CDS             4810..5262
                     /gene="phnG"
                     /locus_tag="ACHJZ2_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO4569215.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5259..5843
                     /gene="phnH"
                     /locus_tag="ACHJZ2_003144"
     CDS             5259..5843
                     /gene="phnH"
                     /locus_tag="ACHJZ2_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO4569216.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5843..6907
                     /gene="phnI"
                     /locus_tag="ACHJZ2_003145"
     CDS             5843..6907
                     /gene="phnI"
                     /locus_tag="ACHJZ2_003145"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO4569217.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6900..7745
                     /gene="phnJ"
                     /locus_tag="ACHJZ2_003146"
     CDS             6900..7745
                     /gene="phnJ"
                     /locus_tag="ACHJZ2_003146"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO4569218.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7742..8500
                     /gene="phnK"
                     /locus_tag="ACHJZ2_003147"
     CDS             7742..8500
                     /gene="phnK"
                     /locus_tag="ACHJZ2_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO4569219.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     SGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8611..9291
                     /gene="phnL"
                     /locus_tag="ACHJZ2_003148"
     CDS             8611..9291
                     /gene="phnL"
                     /locus_tag="ACHJZ2_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO4569220.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9288..10424
                     /gene="phnM"
                     /locus_tag="ACHJZ2_003149"
     CDS             9288..10424
                     /gene="phnM"
                     /locus_tag="ACHJZ2_003149"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO4569221.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10424..10981
                     /gene="phnN"
                     /locus_tag="ACHJZ2_003150"
     CDS             10424..10981
                     /gene="phnN"
                     /locus_tag="ACHJZ2_003150"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO4569222.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10968..11403
                     /gene="phnO"
                     /locus_tag="ACHJZ2_003151"
                     /pseudo
     CDS             10968..11403
                     /gene="phnO"
                     /locus_tag="ACHJZ2_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            11405..12163
                     /gene="phnP"
                     /locus_tag="ACHJZ2_003152"
     CDS             11405..12163
                     /gene="phnP"
                     /locus_tag="ACHJZ2_003152"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO4569223.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12240..12569)
                     /gene="yjdP"
                     /locus_tag="ACHJZ2_003153"
     CDS             complement(12240..12569)
                     /gene="yjdP"
                     /locus_tag="ACHJZ2_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO4569224.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(12638..14908)
                     /locus_tag="ACHJZ2_003154"
     CDS             complement(12638..14908)
                     /locus_tag="ACHJZ2_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4569225.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSG
                     QRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEA
                     DVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEAL
                     LHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLT
                     QRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQL
                     ENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQAVK
                     SQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAA
                     KNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAA
                     SAEIDIFISDLMLPGGMSGAEVVNTARKLYPHLTLLLISGQDLRPSHNPALPDVALLR
                     KPFTRAQLAQALGQEN"
     gene            15022..16542
                     /locus_tag="ACHJZ2_003155"
     CDS             15022..16542
                     /locus_tag="ACHJZ2_003155"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4569226.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16539..17513
                     /locus_tag="ACHJZ2_003156"
     CDS             16539..17513
                     /locus_tag="ACHJZ2_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO4569227.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            17532..18473
                     /locus_tag="ACHJZ2_003157"
     CDS             17532..18473
                     /locus_tag="ACHJZ2_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4569228.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18528..19211
                     /locus_tag="ACHJZ2_003158"
     CDS             18528..19211
                     /locus_tag="ACHJZ2_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO4569229.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19226..20086
                     /locus_tag="ACHJZ2_003159"
     CDS             19226..20086
                     /locus_tag="ACHJZ2_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFO4569230.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            20083..21084
                     /locus_tag="ACHJZ2_003160"
     CDS             20083..21084
                     /locus_tag="ACHJZ2_003160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO4569231.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHYPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21081..21788)
                     /locus_tag="ACHJZ2_003161"
     CDS             complement(21081..21788)
                     /locus_tag="ACHJZ2_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO4569232.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            22079..24064
                     /gene="yjcS"
                     /locus_tag="ACHJZ2_003162"
     CDS             22079..24064
                     /gene="yjcS"
                     /locus_tag="ACHJZ2_003162"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO4569233.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSQGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5361 a         7012 c         6744 g         4959 t
ORIGIN      
        1 aataaatgga ggcaaaaaga ttattttctt tttgcgtttc ctttcaaatg aaaacgatcg
       61 tcgtctaaaa tcagcagtac ccccgacaaa ctcagggatt ttgtgtataa ttgcggcctt
      121 tttcggcaat ctgccgtttt ttggcgcttt tgccctgctg acttttgagg aaatccacat
      181 gtcattacca cactgcccaa aatgcaactc cgaatacact tacgaagata acggcatgta
      241 catctgcccg gaatgtgcct acgaatggaa cgacgcagaa cctgcacagg aaagcgacga
      301 gctgatcgtt aaagatgcta acggcaatct gctggctgac ggcgacagcg ttaccatcat
      361 taaagatctg aaggtgaaag gtagctcttc gatgctgaaa attggcacca aagtgaaaaa
      421 catccgcctg gttgaaggcg accataacat cgattgcaaa atcgacggtt ttggtccgat
      481 gaaactgaaa tctgagtttg tgaaaaagaa ctgattgtat tgtgatcggt aagccggata
      541 aggcgtttac gccgcatccg gcaacagtgc cagatgcctg atgcgacgct cgcgcgtctt
      601 atcaggccta caaattcccg caccctccgt aggccggata aggcgtttac gccgcatccg
      661 gcaacagtgc cgactgcctg atgcgacgct cgcgcgtctt atcaggcttc cctctcatct
      721 gtataaattt cgaactacac ttaactggct tctcttaact gaggtcacca tcatgccgtt
      781 aagtccctac ctctcttttg ccggtaactg ctccgacgcg attgcctatt atcaacgtac
      841 gttgggcgct gaactgctct ataaaatcag cttcggcgaa atgccaaaat cagcgcagga
      901 cagcgccgag aactgccctt ccggaatgca atttcccgat accgccatcg cccacgccaa
      961 tgtgcgcatt gccggaagcg atatcatgat gagcgatgct ataccgtcag gaaaagccag
     1021 ctactccggt tttacgctgg tgctcgattc gcaacaggtc gaagaaggaa aacgctggtt
     1081 tgataacctt gccgctaacg gaaaaatcga aatggcctgg caggaaactt tctgggcgca
     1141 tggctttggc aaagtcaccg ataaatttgg cgtaccgtgg atgattaatg tcgtcaaaca
     1201 acaaccaacg caataatccg ccgggaggcc cgccctcccg cactgtcatc gaattcccgt
     1261 taactcttca tctgttagtc acttttaatt aaccaaatcg tcacaataat ccgccacgat
     1321 ggagccactt ttttagggag gctgcatcat gcaaacgatt atctgcgtcg aacaactgag
     1381 caaaaccttc aaccagcatc aggcgctgca cgcggttgat ctgaatatcc atcatggtga
     1441 aatggtggct ctgcttgggc cgtccggttc cggcaaatcc acccttttac gtcacttaag
     1501 cggtttgatt accggcgata aatccgccgg tagccatatc gagctgctgg gccgcactgt
     1561 ccagcgcgaa ggccgcctgg cccgcgatat ccgcaaaagc cgcgccaaca ccggctacat
     1621 cttccaacaa ttcaacctgg tgaatcgcct gagcgtactg gagaacgtgc tgattggcgc
     1681 gctcggcagc acgccgttct ggcgcacctg ttttagttgg ttcacccgcg agcagaaaca
     1741 gcgcgcatta caggcgctga cccgcgtcgg tatggcgcac tttgcgtatc agcgtgtttc
     1801 cacgctctcc ggcggccagc agcagcgtgt ggcgattgcc cgcgcgctga tgcagcaggc
     1861 gaaagtgatt ctcgctgacg aaccgatcgc ctcgctggac ccggaatcgg cgcgcatcgt
     1921 gatggacacc ctgcgcgaca tcaaccagaa cgacggcatc accgtggtcg tcacgctgca
     1981 tcaggtggat tacgccctgc gctactgcga acgcatcgtc gccctgcgcc aggggcacgt
     2041 cttctacgac ggcagcagcc aacagtttga taacgaacgt tttgaccatc tctaccgcag
     2101 catcaaccgc gtcgaagaga acgcgaaagc tgcctgacat ccccatcatt gaggaaaacg
     2161 aatgaacgct aagataattg cctcgctggc cttcaccagc atgttcagcc tcagcaccct
     2221 gttaagcccg gcacacgccg aagagcagga aaaggcgctg aatttcggca ttatttcaac
     2281 ggaatcacag caaaacctga aaccgcaatg gacgccgttt ttacaggata tggagaagaa
     2341 gctgggcgtg aaggtcaacg ccttctttgc cccggactat gcgggcatta tccagggaat
     2401 gcgtttcaac aaagtggata tcgcctggta cggcaacctg tcggcgatgg aagcagtgga
     2461 tcgcgccaac ggccaagtct tcgctcagac cgtcgcggcg gatggatcgc cgggttactg
     2521 gagcgtgttg atcgtcaaca aagacagccc gatcaacaac ctgaacgatc tgctggcgaa
     2581 gcggaaagat ctcaccttcg gtaatggcga tcctaactcc acctctggct tcctcgtccc
     2641 cggctactac gtcttctcca aaaacaatat ctccgccagt gacttcaagc gcaccgtcaa
     2701 cgccgggcat gaaaccaacg cgctggccgt cgccaacaag caggtggatg tggcgaccaa
     2761 caacaccgaa aacctcgaca agctgaaaac ctccgcgccg gagaagctga aagaactgaa
     2821 agtgatctgg aagtcaccgc tgatcccagg cgatccgatc gtctggcgta aaaacctttc
     2881 cgaaaccacc aaagacaaga tctacgactt ctttatgaat tacggcaaga cgccggaaga
     2941 gaaagcggtg ctggaacgcc tgggctgggc gccgttccgc gcctccagcg acctgcaact
     3001 ggtgccgatt cgccagctcg cactgtttaa agagatgcaa agcgtgaaag gcaataaagg
     3061 gctgaatgag caggacaagc tggcaaaaac caccgagatt caagcgcagc tggatgacct
     3121 ggaccgcctg aacaacgcgt taagcgcgat gagttcggta agtaaagcga tgcagtaaat
     3181 cgtaggtcgg ataagacgcc ccggtgtcgc atccgacaat gtgcaggcgt tgatgccgga
     3241 tgcggtgcaa gcaccttatc cggcctacag accggagcca aacatgcaaa ccatcaccat
     3301 cgccccaccc aagcgcagct ggttctcgct tctgagctgg gccgttgtac tcgccgtgtt
     3361 ggtcgtctcg tggcagggcg cggaaatggc cccgctcacg ctgatcaaag acggcggcaa
     3421 catggcgacc ttcgccgccg acttcttccc gcccgatttc agccagtggc aggattacct
     3481 caccgaaatg ctcgtcacgc tgcaaatcgc cgtctggggc accgcgctgg cggtggttct
     3541 ctccatcccc ttcggcctga tgagcgccga aaacctggtg ccgtggtggg tttaccagcc
     3601 cgtgcgccgc ctgatggacg cctgccgcgc cattaacgaa atggtcttcg ccatgctgtt
     3661 cgtggtcgcc gtcggcctcg gcccattcgc tggtgtgctg gcgctgttta tccacaccac
     3721 cggtgtgctc tccaaactgc tttccgaagc ggtggaagcg attgaacccg gcccggtgga
     3781 aggcattcgc gccaccggcg ccaacaagct cgaagagatc ctctacggcg tgctgccgca
     3841 ggtgatgccg ctgctgatct cctactccct ctatcgcttc gaatccaacg tccgctcggc
     3901 gaccgtcgtc ggcatggtcg gcgcgggcgg gatcggcgtc acgctgtggg aagcgattcg
     3961 cggtttccag ttccagcaaa cctgcgccct gatggtgctg attatcgtca cggtcagcct
     4021 gctggatttc ctctctcaac ggttgcgtaa gcactttatc tgattagcga ggcattgata
     4081 gctatgcact tgtctacaca tccgaccagc tacccaacac gctatcagga gatagccgca
     4141 aaacttgagc aggagcttcg tcaacactac cgctgcggcg actatcttcc cgccgagcag
     4201 cagctggcgg cgcgctttga ggtaaatcgc cacaccctgc gccgcgccat cgaccaactg
     4261 gtggaaaaag gctgggtaca gcgccgtcag ggcgtcggcg tgctggtgct gatgcgcccg
     4321 ttcgattacc cgctcaacgc ccaggcgcgt tttagccaga atctgctgga tcagggcagc
     4381 catcccacca gcgaaaaact gctgtctgtg ctgcgcccgg cctccggtca cgtcgctgac
     4441 gcgctgggga taaccgaggg ggagaacgtc atccatctgc gcaccctgcg ccgggtcaac
     4501 ggcatcgcgc tctgtttaat cgaccactac ttcgcggacc tcaccctctg gccgacgctg
     4561 caacgcttcg acagcggctc gctgcacgat tttctgcgcg agcaaacggg gattgcactg
     4621 cgccgcagcc agacgcggat cagcgcccgc cgcgcccagg ccaaagagtg ccagcgtctt
     4681 gaaattccga atatgtcccc gctgctgtgc gtgcgcaccc ttaaccaccg tgacggtgaa
     4741 agcagcccgg cggagtactc cgtcagcctg acgcgcgccg acatgattga attcactatg
     4801 gagcactgaa tgcacgcaga taccgcgacc cgccagcact ggatgtccgt actggcacac
     4861 agccaaccgg cagaactggc agcccgcctg aacgcgctga acatcaccgc caactatgag
     4921 gtgatccgcg ccgctgaaac tggcctggta cagattcagg cgcggatggg cggcaccggc
     4981 gaacgctttt ttgccggcga cgccacgctg acccgcgccg ccgtacgtct gactgacggc
     5041 accctcggct acagctgggt gctggggcgc gacaaacagc acgccgaacg ctgcgcgctg
     5101 attgacgcgc tgatgcagca aacccgccat tttcaaaact tatcagaaac gcttattgcc
     5161 ccgctggacg ctgaccgtat ggcacgcatt gccgcacgcc aggccgaagt gaacgccagc
     5221 cgggtcgact tctttacgat ggttcgcgga gacaacgcat gaccctggaa accgctttta
     5281 tgcttcccgt gcaggatgcc cagcacagtt ttcgtcgcct gttaaaggcc atgagcgagc
     5341 cgggcgtgat tgtcgccctg catcagctca aacgcggctg gcagccgctg aatatcgcca
     5401 ccaccagcgt gctgctgacg ctggccgata acgacacgcc ggtgtggctt gccgcgccat
     5461 tgagcaacga tatcgtcagc cagagcctgc gttttcatac caacgcgccg ctggtcagcc
     5521 agccggaaca ggcgaccttc gcggtaacgg atgaggcgat ttccagcgaa cagcttaacg
     5581 ccctttccac cggcaccgcc gttgcgccgg aagcgggcgc aacgctgatt ttacaggtcg
     5641 ccagcctgag cggcgggcgc atgctgcgtc tcaccggcgc gggtattgcc gaagaacgaa
     5701 tgatcgcccc gcagctgccg gagtgcattc tgcacgaact caccgagcgc ccgcacccgt
     5761 tcccgctcgg catcgacctg atcctgacct gcggcgagcg cctgctggct gttccgcgaa
     5821 ccactcatgt ggaggtgtgc tgatgtacgt tgccgtgaaa gggggcgaga aggcgatcga
     5881 cgccgcccac gccctgcaag agagccgacg ccggggcgat accgatttgc ctgaactgag
     5941 cgtcgcccag attgaacagc agcttaatct tgcggtggat cgcgtgatga ccgaaggcgg
     6001 cattgccgac cgcgagctgg cggcgctggc gctgaaacag gccagcggcg ataacgtcga
     6061 agcaattttc ctgctgcgcg cctaccgcac cacgctggcg aagctcgcgg taagcgagcc
     6121 gctcgacacc acggagatgc gtctcgaacg gcgtatttca gcggtttata aagatattcc
     6181 cggcggccag ctgcttggcc caacctacga ctacacccat cgcctgctcg atttcaccct
     6241 gctggcaaac ggcgaagcgc cgacgctgac caccgccgac agcgaacagc agccgtcgcc
     6301 gcacgttttc agcctgctgg cgcgtcaggg gctggcgaag tttgaagagg atagcggcgc
     6361 acagccggat gacatcaccc gcacgccacc ggtttacccc tgctcacgtt cttcccgcct
     6421 gcaacagctc atgcgcggcg acgaaggcta tttgctggcg ctggcctact ccacccaacg
     6481 cggttacggg cgcaatcacc cgttcgcggg tgaaatccgc agtggttaca tcgacgtgtc
     6541 gattgtgccc gaagagctgg gatttgcggt gaacgtcggc gaactgctga tgactgagtg
     6601 tgaaatggtc aacggtttta tcgacccacc ggatgaatct ccgcacttca cgcgcggcta
     6661 cgggctggtg ttcggcatga gcgagcgcaa agcgatggcg atggcgctgg tcgaccgcgc
     6721 tctgcaagcc ccggagtacg gcgagcacgc gacaggcccg gcgcaggatg aagagttcgt
     6781 gctggcacat gccgacaacg tcgaagccgc aggctttgtc tcgcacctca aactcccgca
     6841 ctacgtcgat ttccaggccg aactggagct actcaaacgt ctgcaacagg agcagaacca
     6901 tggctaatct gagcggctac aactttgcct acctcgacga gcagaccaaa cgcatgatcc
     6961 gccgcgccat cttaaaagcg gtggcgatcc ccggttatca ggtgccgttt ggcgggcgcg
     7021 agatgccaat gccgtacggc tggggaaccg gcggcattca gcttaccgcc agcgtgattg
     7081 gcgaaagcga cgtgctgaag gtgattgacc agggcgcgga tgacaccacc aacgccgtgt
     7141 cgattcgcaa cttctttaag cgcgtgaccg gggtaaacac caccgagcgc acggacgatg
     7201 caacggtcat ccagacgcgt caccgcatcc ccgaaacgcc gctgaccgaa gatcagatca
     7261 ttattttcca ggtgccgatc cccgaaccgc tgcgctttat cgagccgcgc gaaacggaaa
     7321 cccgcaccat gcacgcgctg gaggaatacg gcgtgatgca ggtgaagctg tatgaagata
     7381 tcgcccgctt cggtcatatc gccaccacct acgcctatcc ggtgaaggtg aacgggcgct
     7441 acgtaatgga cccgtcgccg atcccgaaat tcgataaccc gaaaatggac atgatgcccg
     7501 ccctgcaact cttcggcgcc gggcgtgaga agcgcatcta tgcggtgccg ccgtttaccc
     7561 gcgtggaaag cctcgatttc gacgatcacc cgttcaccgt tcagcagtgg gatgagccat
     7621 gcgccatctg cggatcgacc cacagctatc ttgatgaagt ggtgctggat gacgccggaa
     7681 accgcatgtt tgtctgctcc gataccgatt attgccgcca acagagcgag gcaaaaagcc
     7741 aatgaatcaa ccgttacttt cggtcaataa cctgacccac ctttacgcgc cgggcaaagg
     7801 ctttagcgat gtctcttttg atttatggcc gggggaagtg ctgggcattg tcggggaatc
     7861 cggctccggg aagaccacgc tgctgaaatc aatttccgcg cgtctgacgc cgcagcaggg
     7921 ggagattcac tatgaaaacc gttccctcta tggcatgagc gaggccgacc gccgtcgcct
     7981 gctgcgcacc gaatggggcg tagtgcatca gcatccgctc gacggcctgc gccgccaggt
     8041 ctcggcaagc ggcaatatcg gcgagcggct gatggcgacc ggggcgcgtc attacggcga
     8101 tattcgcgcc accgcgcaga agtggctgga agaggtggaa attcccgcca accggatcga
     8161 cgacctgccg accacctttt ccggcggtat gcagcagcgt ttgcagattg cccgcaacct
     8221 ggtgacgcat ccgaagctgg tgtttatgga tgaaccgacc ggcgggctgg atgtgtcggt
     8281 gcaggcccgc ctgctcgacc tgctgcgcgg cctggtggtg gagctgaacc tcgcggtagt
     8341 gattgtcacc cacgatttag gcgtcgcccg cctgctggcg gaccgtttgc tggtgatgaa
     8401 gcaggggcaa gtggtggaga gtgggttaac cgaccgcgtg ctcgacgacc cgcatcatcc
     8461 gtatacacag ctgctggtgt catcggtttt gcagaattga gccggtgccg gatgcggcgt
     8521 aaacgcctta tccggcctac aaatgcgctc cccgtaggtc ggataagacg catcagcgtc
     8581 gcatccgaca cccgaaccac gaggcgaaaa atgattaacg tacaaaacgt cagtaaaacc
     8641 ttcatcctgc accagcaaaa cggcgtgcgc ttgcccgtcc tcaaccgcgc ctcgctcacc
     8701 gtcaacgcgg gcgaatgcgt ggtgctccac ggccactccg gcagcggcaa atcaaccctg
     8761 ctgcgctcgc tgtacgccaa ctatctgccc gatgaagggc aaatccagat caaacacggt
     8821 gacgagtggg tagacctggt cactgcgcca gcgcgcaaag tggtggaaat ccgcaaaacc
     8881 accgttggct gggtgagcca gtttctgcgc gttatcccgc gcatttcggc gctggaagta
     8941 gtaatgcagc cgctgctcga taccggcgta ccgcgtgaag cctgcgccgc taaagccgcg
     9001 cgtctgctca cccgtctgaa cgtgccggaa cgcctgtggc gccttgcgcc atcgaccttc
     9061 tccggcggcg aacagcagcg cgtcaacatc gcccgtggct ttatcgtcga ctaccccatt
     9121 ctgctgctcg acgaacctac cgcctcgctg gacgccaaaa acagcgccgc cgtagtcgaa
     9181 ctgatccgag aagccaaagc ccgcggcgca gccatcgtcg gcattttcca tgacgaagcc
     9241 gtacgtaatg acgtcgccga ccgcctgcac ccaatgggag cctcttcatg attatcaata
     9301 acgtcaaact ggtgctggaa aacgaagtgg taaacggttc gctggagatg caggacggcg
     9361 aaatccgcgc ctttgccgaa agccagagcc gcctgccgga ggcgatggac ggcgaaggcg
     9421 gctggctgct gccggggctg attgagctgc atacagacaa cctcgataaa ttcttcaccc
     9481 cgcgcccgaa agttgactgg cccgcccact cggcgatgag cagccatgac gcgctgatgg
     9541 tggcgagcgg catcaccacc gtactggacg ccgtggcgat tggcgacgtg cgagacggcg
     9601 gcgatcggct ggagaatctg gagaagatga tcaacgccat cgaagagacg cagaaacgcg
     9661 gcgtcaaccg cgccgagcac cgcctgcacc tgcgctgcga gctgccgcat aacaccacgc
     9721 tgccgctgtt tgaaaagctg gtgcagcgcg agccggtgac gctggtgtcg ctgatggatc
     9781 actcgccggg ccagcgccag ttcgccaacc gcgagaagta tcgcgaatat tatcagggca
     9841 aatactcgct caccgatgcg cagatgcagc agtatgaaga ggaacagctg gcgcttgccg
     9901 cacgctggtc gcagccgaat cgcgaatcca tcgccgcaat gtgccgcgcg cgacaaattg
     9961 cgctcgccag ccacgatgac gcaacgcacg cccacgttgc cgaatctcac cagcttggca
    10021 gcgtgatcgc cgaatttccc accacgtttg aagcagcgga agcctcgcgc aagcatggca
    10081 tgaacgtgct gatgggcgcg ccaaatatcg tgcgtggcgg ttcgcactcc ggcaacgtgg
    10141 cggccagtga actggcacag cttggcctgc tggatatcct ctcttccgac tactaccccg
    10201 ccagcctgct ggatgcggcg ttccgcgtcg ccgatgacca gagcaaccgc tttacgctgc
    10261 cgcaggcggt gaagctggtg accaaaaatc cggcgcaggc gcttaatctc caggatcgcg
    10321 gggtgattgg cgagggcaaa cgcgccgacc tggtgctggc gcatcgcaag ggcaatcaca
    10381 ttcatatcga ccacgtctgg cgtcagggta aaagggtgtt ctgatgatgg gaaaactgat
    10441 ttggttaatg gggccgtccg gctccgggaa agatagcctg ctggcggaac tccgcctgcg
    10501 ggaacaaact cagctactgg tggcgcatcg ctacatcacg cgcgacgcca gcgccggaag
    10561 cgaaaaccat atcgccctga gcgagcagga attttttacc cgcgcggggc aaaatctgct
    10621 ggccttaagc tggcacgcta acggtctgta ttatggcgtc ggcgtcgaga ttgacctctg
    10681 gctgcacgct ggattcgacg tgctggtcaa cggctcacgc gcccatctgc cgcaggcgcg
    10741 ggcgcgctat caatcggcgc tgctgcccat ttgcttacag gtttcgccag agatcctgcg
    10801 ccagcgcctg gaaaaccgtg gccgcgaaaa cgccagtgaa attaacgccc ggctggcgcg
    10861 cgccgcccgc tatactccac aggattgcca tacgctcaat aatgacggca gcctgcgcca
    10921 gtcggtcgac acgctgctga cgctaatcca tcagaaggag aaacaccatg cctgcttgtg
    10981 agctttcgcc cggccacgca gtacgacacc gacgcggttt acgcgttgat ttgcgagcta
    11041 aaacaggcgg agtttgacca ccacgcgttt cgcgtgggct ttaacgccaa cctgcgcgac
    11101 ccgaacatgc gctaccatct ggcgctgctg gatggcgaag ttgtcggtat gatcggcctg
    11161 catttgcagt ttcatctgca tcatgtcaac tggatcggcg aaattcagga gttggtggta
    11221 atgccgcagg cgcgcggtct gaacgtcggc agtaagttac tggcgtgggc agaagaagaa
    11281 gcccgccagg ccggggccga aatgaccgaa ctttcgacca acgtgaagcg ccacgacgcg
    11341 caccgtttct atctgcgcga aggctacgag cagagccact tccgcttcac caaggtgctg
    11401 taacatgagc ctgaccctca cgctcaccgg ctccggcggc gcacagggcg ttccggcatg
    11461 gggctgcgag tgtgcggcct gcgccagagc gcggcgctcg ccgcactatc gccgccagcc
    11521 atgcagcggc gtagtgaagt ttaacgaggc gatcaccctg atcgacgccg ggcggcacga
    11581 tctcgccgat cgctggtcgc ccggatcgtt ccagcagttt ttgctgacgc actaccatat
    11641 ggatcacgtg caggggctat tcccgctgcg ctggggcgtg ggcgatccga tcccggttta
    11701 cggcccaccg gatgaacagg gctgcgacga tctgtttaaa catccgggcc tgcttgattt
    11761 cagccacacg gtggaaccgt ttgtggtgtt tgatttgcag gggttacagg tcacacccct
    11821 gccgctcaac cactcaaaac tgaccttcgg ttatctgctg gaaacggcgc acagccgggt
    11881 ggcgtggctg tctgacaccg ctggcttgcc ggaaaaaacg ctgaaatttt tacgcaataa
    11941 tcagccgcag gtaatggtga ttgattgcag ccacccgccg cgcgaggatg caccgcgtaa
    12001 tcactgtgat ttaaataccg tgcttgcgct gaatcaggtt atccgctcgc cgcgcgtggt
    12061 tctgacccat atcagccacc agtttgatgc gtggctgatg gaaaacgcac tgccgtcggg
    12121 gtttgaggcg gggtttgatg ggatggagat tggggtggcg tgatgagagg gaatgtgcgt
    12181 gctggccccc tcaccctaac cctctcccca gaggggcgat gcctgcccca tacccaacat
    12241 cattgcccat actcatcttc cattctccgc tcttcatctt ctagctgccg acgctcctga
    12301 ttaagctggt gctggcgatc gtccagctgc ctgcgacgat cttcaaactg gcgacggcgg
    12361 tcgtcatatt gtctgcgccg atcgtcgctc acttcacgct gccagccgtc gtcgcgcgaa
    12421 tcttcataat ctcgcccacg gtcagggtta gaagcgtcgt taatcgcctg ctgaatattg
    12481 ccaatggtgt catcgataat atcggcctgg gccggtacgg tggtcagcgt gagcagggtg
    12541 aaaagaaaaa gcgtggagtg gcgtttcata aactcggcct cgcaacaaag ttaatacaag
    12601 gtaggcaaag gtgcctgtta cgagtagagg aggattctca attctcttgc cccagcgcct
    12661 gcgccaactg cgcacgggta aagggtttgc gcaacagcgc cacgtctggc agcgcggggt
    12721 tatggctggg gcgtaaatcc tgaccgctga tgagtagcag cgtcaggtgc gggtaaagtt
    12781 tgcgggccgt attgaccact tctgcgccgc tcatgccgcc ggggagcatt aaatcgctga
    12841 taaaaatatc gatttctgct gatgccgcca gcagatccag cgcctgttcg ccgctgcttg
    12901 cttccagcgt caaatagccc agcaggtgca actgttcgca gatggtctgg cgcaccgcgg
    12961 cttcatcttc cagcactaac accagtttat cgccgctgac agaagcctgt tctactgccg
    13021 ctggcgatag gtttttcgcc gccgtcagcg cgcggggaag ctgtaatctg accgttgtcc
    13081 cctgccccgg cgcgctttca atttcaacgc gcccaccgga ctggcgcaca aaaccataga
    13141 ccatcgacaa acccagcccg ctgccgctgc ccgtctgttt ggtggtaaag aatggctcaa
    13201 atacctggct tttcaccgcc tgcgacatac cgcagccatg atcgatgacc tccagtgcca
    13261 ccatgtcctg acgctgccca ctgctgcgag taacgcgctg attccaggta cgaattttga
    13321 tgattcccgt ctgcccttcc atcgcgtcac gggcgttcat caccaggttg atgatggcat
    13381 tttccagttg gctgatgtca atccacgcgg gccacgccgg agactgcgct tcaatctcca
    13441 gcgccagcgt tgccggtaag gagtggcgca ttaattcgct taactcttcc agcaatgttt
    13501 tcagttcaac ggcttgcgga tgtaaggact gcttgcggga aaacgccagc agtcgttgtg
    13561 tcagtagcgc cccacgttcg gcagcttgca gcgcccgctg tattcgcggc gcgtctggcg
    13621 agtccggatt caccagctcc aggctgccaa taatcaccgc cagcagatta ttaaaatcat
    13681 gggccagccc gcccgtcagt tgcccgacag ctttcagttt ctggctgtgt aatagcgcct
    13741 cttccaaccc ctgccgctca gtttgggcaa tctccatctg cgtggtcttc tcttttagca
    13801 gccgtgaggt tcgttcgagt gacgcggtat tgcgggcaaa aacgttaaag gcgcgcgcca
    13861 gttcacccaa ctcatcacgc cgctgtaacg ccggaaaagt gacgtccgct tcgccgtgcg
    13921 ccaggcggct catggcgcgg gaaatcgccg tcaggttagt gccgaggttg cgatagatat
    13981 accagccggc aaacgcggtg atcaccaccg ctaacagggc gaaaatcatg atcaccctct
    14041 cgccggagcg caaatcctgg taactttgcg ccacacgctg accggagagc gtcgccacct
    14101 gttcggcgta acggttgatg tcgccattga ggatcgccac cagcgctttg atgtgaaaca
    14161 tataccagct gattgccaga tcgctttgct ccagagcggc agaaagcggc gcgagtttac
    14221 gcatttcatc attgaaatca ttgaaatcat tgagtaacgt attaaccaat ggcgtatcgc
    14281 tgtgggtcgg caacgcagac attaccccga ccagttgatg aataatcgcc tgcggcgcgg
    14341 gggtggcaat agcggcaaca atcagtcggt tcatctcgtt aagcaaaata tcatcttgcg
    14401 cgtgagtgac tttttgcaga tgccgcagat aacttaagtt ttgatacagc gaacttaaca
    14461 gcgcgttgcg ctccagatgc cgcctttgcc cgcgctccag cataccgccg acgctggttt
    14521 gcagttcgtt gctacgcgtg atgattcgcg tcaccagccc ggcttcctgc tgcgctaaag
    14581 gcgcgttcgc cagttgtgca agcgagcttt ttaatgcccg ctgtgtctcc tgcaaccgct
    14641 cggcttcgcc tttatattcc agcgctccca ccacctgcga caatcgcacc gccgccgttg
    14701 ccacgtttgc cgtgtcgcgc gccagattca ggctgccggt catatcatcc accgtttgct
    14761 gctgcaccag ctcctgcatc tggctggcat gacgaaagcc cattaccgcc acgccgctga
    14821 ccatcaacgt caccgccacc accagcaaat tcaaaagcag caatcgcccg cgcgcgctgg
    14881 caaaaaagtg acgtccgggg tgcgccatat cacgctccgt gaagaaaaat cgcaatatga
    14941 caaatatgaa cggcttctga cattttgcat ttactttcct gtgatggagt gctgatatcg
    15001 acaacctcgc aggagtccac catgaacagc acgccagttc tggagatgcg caatattgcc
    15061 aaagccttcg gcaaatttta tgcactcaaa ggggtggatt tgacggtcta ccctggcgag
    15121 atccatgccc tgatggggga aaacggcgcg ggaaaaagca cgctgatgaa ggtgctggcg
    15181 ggggcgtata ccgccaccag cggcgagatt ctcatagacg gcaagccctt tcacattcgc
    15241 acgccaaaag atgccttaag cgccggtatt acgctgattt atcaggagat gcagctggca
    15301 ccgaatctgt cggtggcaga aaatattttt ctcggcagcg agctttccca cggcgggctg
    15361 gtgcagcgta aagagatgct agtgcaggcg caaaaagtga tcgaccgcct cggcgcgcag
    15421 tttaacgcca gcgataaggt catgacgctg accattgccg agcaacagca ggtcgaaatc
    15481 gcccgcgcac tacatcgcaa cagccgcatt ctggtgatgg acgaacccac cgctgccctc
    15541 tcctcccgcg aaactcaccg cctgtttgaa ctgattatgc ggctgcgcga tgaagggatg
    15601 gcgattatct acattagcca ccgcatggcg gaagtgtatg agctttccga tcgcgtcagc
    15661 gtgctacgcg acgggcaata cgttggcagc ctgacccgcg ataacctcaa tgccggggag
    15721 ctggtgcgga tgatggtcgg caggccactg agcgatctgt tcaataaaga gcgcgatatc
    15781 ccgctcggta aagcccgcct gaatgttcac cacctgaccg acggcggcaa agtccagccg
    15841 agtagcctgc tggtgcgttc cggcgaaatt gttggcctcg ccggactggt gggtgccgga
    15901 cgttccgaac tggcgcagtt gatcttcggc gtgcggaaag cgacaggcgg aatgattgaa
    15961 gtcgatggtg aaccggtggt gatccactcc ccgcgcgaag ccatcgatct tggcattggt
    16021 tttctcaccg aaaaccgcaa agaacaaggc ttattccttg aaatggcagc agccgaaaac
    16081 atcaccatgg caaccctgga gcgcgatgcc cgctggggaa tgctcaatcg caaaaaagcg
    16141 caaaccattt ccgatgacgc cattaagttg ctcaacattc gcgtgcctca tgcccaggta
    16201 cgcgcgggcg ggctttccgg cggcaatcag caaaaactgt tgatctcccg ctgggtggcg
    16261 attggcccgc gcattttact gctcgatgaa cccgcccgcg gcgtggacgt tggcgccaaa
    16321 agtgagatct accggatcat gaacgagatg gcgcgcaagg gcgtggcgat cctgatgatc
    16381 tccagcgaac tgccggagat agtgggaatg agcgatcgcg tctatgtgat gcgcgaaggc
    16441 agcattgccg gtgagttaaa cggcaaaaac atcacccagg aaaacattat gaccttagcg
    16501 actggcgtca acgacgccca ttcccaggcg gtaacctcat gacgacctct gcaaaaacct
    16561 ctgccaaaaa aatgctgatg agcgatctga tgcaaacggt cggcatcttg cccattttga
    16621 ttctgatcgt ggcggtattt ggctttatca cgccaaactt ttttaccgaa agcaacctgc
    16681 tgaatattac ccgccaggca tcgatcaaca ttgttctggc ggcggggatg accttcatca
    16741 ttttgaccgg cgggatcgat ctttccgtgg gttcgatcct cggcaccaca gcggtggcgg
    16801 cgatggtcgt ctcccttagc cctgagttcg ccatgctgtc gatcccggca gcgctgatgc
    16861 tcggcctgct gctggggctg ttcaacggcg cgctggtggc ctttgccgga ttgccaccgt
    16921 ttatcgttac gcttggcacc tatacggcgc tgcgcggggc ggcgtatctg ctggcggacg
    16981 gcaccacggt tatcaactcc aacattaatt tcgagtggat aggcaacaac tatctcgggc
    17041 cggtcccatg gctggtggtg attgcgctgg cggtgatcgt ggtgtgctgg tttattctgc
    17101 gccgcaccac gcttggcgtt catatctacg ccgtcggcgg caatatgcag gcggcgcgtc
    17161 ttaccggcat taaggtctgg ctggtgctgc tgtttgttta tggcatgagc ggcctgcttt
    17221 ctgggcttgg tgggattatg agcgcctcac ggctgtacag cgccaacggc aacttaggca
    17281 tgggttacga actggatgcc attgcagcgg tgatcctcgg cggcaccagt tttgtcggcg
    17341 gcatcggcac catcaccggc acgctggtcg gcgcgctaat tatcgccacg ctcaacaatg
    17401 gcatgacctt aatgggcgtc tcttacttct ggcaactggt gatcaaaggg gcgatgatca
    17461 tcattgcggt gctgatcgac aaataccgta cccgacacca tcaaagtgca taaacacatg
    17521 aggaatacag catgagattg aagcccttag tgaccgcgct ctgtgcaggc gcgctgctgg
    17581 ccgcagcacc gttcgcccag gcgaaagaac tgaaatcaat aggcgtaacg gtgggcgatc
    17641 tggcgaaccc gttcttcgtg cagatcacca aaggcgcgga gctggaagca cgcaaactgg
    17701 caggcgataa cgtcaaagtg acgctggttt ccagcggcta cgatctgggt cagcaggtgt
    17761 cgcagataga caactttatc gccgctaacg tcgatatgat tatcctcaac gctgccgatt
    17821 caaaagggat cggcccggcg gtgaaacggg cgaaagatgc cgggatcgtg gtcgtcgcgg
    17881 ttgacgtagc ggcagaaggt gccgatgcca ccatcacttc cgataacacc caggcaggcg
    17941 aaatggcctg taaatacatc accgatcgcc tgaaaggcaa aggcaacgtg gtgatcatca
    18001 acggcccgcc ggtatccgcc gtgcaaaacc gcgtcgaagg atgccagact gaatttaaac
    18061 gccatcctga catcaaagtg ctctctgaca accagaacgc gaaaggcagt cgtgaaggcg
    18121 ggctggaggt aatgacggga ctgctggcgg caaatccgaa gatcgacggc gtatttgcga
    18181 ttaacgatcc caccgcaatc ggtgccgatc tggcagcaaa acaggcacaa cgtaacgagt
    18241 tctttattgt cggtgtggac ggaagcccgg acgccgaaga ggcgctcaag cgtgagaact
    18301 cgttgtttgt cgctactccg gcgcaagatc cccaggtgat ggcagcgaaa gcggtggaaa
    18361 ttggctacga cattttgcag ggcaaacctg cgccgaaaga gccggtgctg atcccggtga
    18421 cgatgatcga taaaaacaac gtcggcaatt acaaaggctg gacggtgaaa taagtccaca
    18481 cgccgggcgg cgcagctgcc cggtcaccgc taaggagaac gctttcgatg aaacgctccg
    18541 ctataaacga cattctcggc catacacggc aattcttttc gcaacatgac gtacatctgc
    18601 cgccctttgc cagtttctcg cctgctcaat ggcagcagct cgacaccgcc gcgtgggagg
    18661 aggtttttga tcttaaactc ggctgggatg ttaccgcttt tggtcgcaac aactttgcag
    18721 cacacgggct gacgctgttt accctacgta acggctcagc aaaaggaatg ccgtatgtga
    18781 aatgttacgc cgagaagatc atgcatgtgc gcgatgcgca ggtcacgcca atgcattttc
    18841 actggcgtaa gcgtgaggac attatcaatc gcggcggcgg taatttaatt gttgaactgt
    18901 ggaatgccga tagtaacgag caaacggctg atagtgacat tacggtggtg atagacggct
    18961 gccgccagaa acatactgca ggctctcagt tgcgcctgtc tcccggcgaa agtatctgcc
    19021 tgccgcccgg cctgtatcac agcttttggg cagaagccgg ttttggcgat gtgctggttg
    19081 gcgaagtctc ttctgttaat gacgacgacc acgataacca ctttttacag ccactggacc
    19141 gctacaacct gattgacgaa gacgaacctg cacagttggt attgtgtaac gaataccgcc
    19201 agttccgctg aataaggata acgttatgcc attgatttct ctcgctgaag ggctggcaca
    19261 tgcccgcgaa catcactatt cgttaggtgc gtttaatgtg ctggactcgc actttttacg
    19321 cgccttgttt gctgctgcca gacaagaacg ttcgcccttt attatcaaca ttgccgaagt
    19381 acattttaaa tatatctcgc tggattccct ggtcgagaca gtgaaatttg aagcggcgcg
    19441 tcatgacatt ccggtggtgt taaatctcga tcacggactg cattttgaat cggtggtgca
    19501 agcgttgcgg ctgggattca gctcggtaat gtttgatggc tccacgctgg ggtatgaaga
    19561 aaatgtccgc cagacgcggg aagtggtgaa aatgtgccat gccgttggcg tatcggtaga
    19621 agctgaactg ggcgcggtgg gtggcgacga aggcggtgca ctctatggtc atgccgatga
    19681 atccctgttt accgatccgc aactggcgcg tgattttgtc gaccgtaccg gcattgacgc
    19741 gctggccgtg gcgattggca atgcacacgg caaatatcgc ggcgaaccga aactcgattt
    19801 cccgcgcctc gacgctatcc gccagcaggc tgctatcccg ctggtattac acggcggctc
    19861 agggattagc gatgccgatt tccgccgcgc catcgaactg ggtatccaca aaatcaattt
    19921 ctacactggc atgtcgcaag cggcgctggc tgccgttgag gcccgaatgg cgcaccgtca
    19981 gccgatgtat gacgagtttg ccgagttact gctggcagtg gaagccgcca tcagcgatac
    20041 ggttgcacaa cagatgcgta tttttggcag tgcggggcag gcatgatgga gcgtaaaggg
    20101 attatcgccg caggcaatat gttggttgac cacgtccatc agattatcca gtggccagag
    20161 cgcggctggc tggcggagat cacccacagc gaacgctcca ccggcggtgc gccgcttaac
    20221 gtcctgctga cgctggcgaa aatgcgcacc gggctgccgt tacaggcggt tgggctaatc
    20281 ggtcaggaca gcgacggcga ttacatcatg gcgatgcttg agcaatatca cgtcaatcgt
    20341 cagcatgtgc agcgcactac ctttgccccg acgtcgatgt cgcaggtgat gacagaccca
    20401 agcgggcagc ggacgttttt ccattcgccg ggtgcaaaca gacttttgga tcttccggcg
    20461 ttcgatcagc tcgataacac catgaagatt ttccatctcg gctatttact gttgctcgac
    20521 agcctggata tgccggacga tgtttacggc acccgtagcg cacgcctgct ggcgcaaatg
    20581 cgtgatactg gctttgaaac ctcactcgac ctggtctccc gtaagggcga tccgcgttat
    20641 cagccgctgg tgctgcccgc gttgcgccat ctggattatc tggtaattaa cgaactggag
    20701 gccggggagt ttagcgggct ggaaattcgg ttacccaacg gcgaaccgca tatcgcgcat
    20761 attgctgccg ccgcgcgtga actgttagat gcgggcgtgc gccagcgggt ggtgatccac
    20821 tacccggaag gggcgtgggg agagacccca gaacagggcg gcgtgtggat cccttcgcaa
    20881 aagttagaac aacgggaaat tatcggcagc gtcggcgcgg gcgatgcatt ttgtgccgga
    20941 tttttatatg gctgccatga acgctggacg cttaccgaga gcatcaaact ggcacacgcc
    21001 tgcgcgcggg caagcctgtt atgcgccaat gccatcgacg gggcgaaaac gctggaagag
    21061 ctgcaaacag aaatgtcgga ttaatgcacg tcggcagcga agacataccc cagaccgcgc
    21121 agagttttaa tcaacatcgg ctgatgcggg ttgagttcga ttttgcgtcg taagcgcatg
    21181 attaatacgt cgatggtgcg atcaaacacc tccacgctgt cgctgtgggt gaacgccagc
    21241 aactgctcgc ggctcaatac ccgacgcgcg ttttgcgcca gagccagtaa caagccatat
    21301 tcgccctgag ttaactcgac atgttgctga agcggattgt gcaactggca gcgcatggta
    21361 tcgagatacc agccattgaa tgacagcccg gcgcttttct cgtcattaac cgccagcgcg
    21421 ccaacccgtc gcaacaccgc tttcagtcgt gcgaccacca cccgcggggt gaacggtttg
    21481 cagatgtaat catccgcgcc catttccagc ccgaccacca catcagactc actgcccatg
    21541 ccggtcaaca tcaccaccgg caatgtcgga cgcagtttct ggatttgctg caataccctc
    21601 aggccgttgg tatcgggtag catcatatcc agcaccacca gcgcaatgtc aggatcgccc
    21661 tcaatacgca atatggcctc ctgtccggta tggcagactg aaaccgtaaa gacatgttca
    21721 cttaacacgt cctgcaacag tgcgcagata gccgtgtcat catccaccac cagcacgacg
    21781 ggtttcatgg ttacactccg catcattcat ctgattaatc attgtgcgta cttcttgtcg
    21841 aagtgaaagc tgaaagtaat ataaataata aaaatataag tcaggtcaca ttattgatga
    21901 tagtaataaa tatgtcgtac ttttccctct tcagcacaaa tgcctaataa aatggatcgt
    21961 tccgctcaca cttccactta ttaaagtcgc aatttcccaa ttatgataac gccagcttta
    22021 aatgacgtat tttgcatgga ttacatattt acgacataaa tgataaatgg agatttatat
    22081 gaataactct cggttattcc gtttgagcag gattgttatt gcgttaactg ccgccagcgg
    22141 catgatggta aataccgcta acgcgaaaga ggaagcgaaa gccgccactc aatataccca
    22201 acaggttaat cagaattacg ccaaatcatt accgtttagc gatcgtcagg attttgacga
    22261 tgcccagcgt ggatttatcg ccccgctgct ggatgaaggt attctgcgtg atgcgaacgg
    22321 taaagtttac taccgcgcag acgattacaa atttgatatt aatgccgctg cgccggaaac
    22381 cgtaaacccc agcctgtggc gtcagtcgca gatcaacggc atttctggcc tgttcaaagt
    22441 caccgataaa atgtatcagg tgcgcggcca ggatatctct aacattacgt tcgttgaggg
    22501 ggagaaaggc attattgtca tcgacccgct ggtgacgccg cctgccgcaa aagccgcact
    22561 tgacctttac ttccagcatc gtccgcaaaa accgattgtt gccgttatct acactcacag
    22621 ccacaccgac cactatggtg gcgtgaaagg cattatctct gaagccgatg ttaaatccgg
    22681 caaagttcag gtgattgccc ctgctggctt tatggacgaa gccatcagcg aaaacgtgct
    22741 ggcgggtaac atcatgagcc gccgtgcgct ctactcttac ggtctgttac tgccgcacaa
    22801 cgcgcaaggc aacgtgggta atggccttgg cgtgacgctg gcaacgggcg acccgagcat
    22861 tattgcaccg actaaaacta tcgtcagaac tggcgagaag atgattatcg acggcctgga
    22921 gtttgacttc ctgatgaccc caggtagcga agcgccagcc gaaatgcact tctatattcc
    22981 ggccctgaaa gccctgtgta ccgccgagaa cgccacgcat accctgcaca acttctacac
    23041 tctgcgcggc gcgaaaaccc gcgataccag caagtggacc gagtacctga acgaaacgct
    23101 ggatatgtgg ggtaacgacg cggaagtact gtttatgccg cacacatggc cggtctgggg
    23161 caataagcat atcaatgatt atattggtaa atatcgcgat actatcacgt tcattcacga
    23221 ccagaccctg cacctggcga accagggcta caccatgaat gaaatcggcg acatgattaa
    23281 gctgccgcct gcacttgcca ataactgggc cagccgtggc tattacggtt ctgtcagcca
    23341 caacgcccgc gcggtgtata acttctatct tggctattac gacggtaacc cggctaacct
    23401 gcatccgtat ggtcaggtag agatgggcaa acgttacgtg aaagcgctgg gcggctctgc
    23461 ccgtgtcatc aacctggcgc aagaagcgaa taagcaaggt gattaccgct ggtcggcaga
    23521 actactgaaa caggtgattg ccgccaaccc gggtgaccag gtggcgaaga atctgcaagc
    23581 gaataacttt gaacagctgg gctatcaggc cgagtccgcc acatggcgcg gtttctacct
    23641 gaccggcgcg aaagagctgc gcgaaggggt gcataagttc agccagggca ccaccggttc
    23701 cccggacacc attcgcggaa tgtcggtcga aatgctgttc gactttatgg ccgttcgcct
    23761 cgatagcgcg aaagctgcgg gtaaaaatat cagcctgaac ttcaatatga gcaacggcga
    23821 taacctcaac ctgacgctga acgatagcgt gcttaactac cgtaaaacac tgcaaccgca
    23881 agccgacgcc tctttctaca tcagccgtga agatctgcac gccgtgctga ccggacaggc
    23941 aaaaatggcg gatctggtga aagcgaagaa agccaaaatt attggcaatg gcgcgaaact
    24001 ggaagaaatt atcgcctgtc tggataattt cgatttgtgg gtgaatatcg taaccccaaa
    24061 ttaatgtcac tcgctt
//
LOCUS       DAZFBR010000075        23938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFBR010000075 DAZFBR010000000
VERSION     DAZFBR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.075"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(76..1341)
                     /locus_tag="ACHJZ2_003163"
     CDS             complement(76..1341)
                     /locus_tag="ACHJZ2_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4569234.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1539..1614)
                     /locus_tag="ACHJZ2_003164"
     tRNA            complement(1539..1614)
                     /locus_tag="ACHJZ2_003164"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Phe,seq:gaa)
     gene            complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ACHJZ2_003165"
     CDS             complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ACHJZ2_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO4569235.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2580..2663
                     /locus_tag="ACHJZ2_003166"
     CDS             2580..2663
                     /locus_tag="ACHJZ2_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569236.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            2825..4960
                     /gene="speC"
                     /locus_tag="ACHJZ2_003167"
     CDS             2825..4960
                     /gene="speC"
                     /locus_tag="ACHJZ2_003167"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO4569237.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ACHJZ2_003168"
     CDS             complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ACHJZ2_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO4569238.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ACHJZ2_003169"
     CDS             complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ACHJZ2_003169"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO4569239.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ACHJZ2_003170"
     CDS             complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ACHJZ2_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO4569240.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ACHJZ2_003171"
     CDS             complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ACHJZ2_003171"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO4569241.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9128..9847
                     /gene="trmB"
                     /locus_tag="ACHJZ2_003172"
     CDS             9128..9847
                     /gene="trmB"
                     /locus_tag="ACHJZ2_003172"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO4569242.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9847..10173
                     /gene="yggL"
                     /locus_tag="ACHJZ2_003173"
     CDS             9847..10173
                     /gene="yggL"
                     /locus_tag="ACHJZ2_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO4569243.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10357..11076
                     /gene="yggN"
                     /locus_tag="ACHJZ2_003174"
     CDS             10357..11076
                     /gene="yggN"
                     /locus_tag="ACHJZ2_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO4569244.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11252..12298
                     /gene="ansB"
                     /locus_tag="ACHJZ2_003175"
     CDS             11252..12298
                     /gene="ansB"
                     /locus_tag="ACHJZ2_003175"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO4569245.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12415..13422
                     /gene="yggM"
                     /locus_tag="ACHJZ2_003176"
     CDS             12415..13422
                     /gene="yggM"
                     /locus_tag="ACHJZ2_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO4569246.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="ACHJZ2_003177"
     CDS             complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="ACHJZ2_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO4569247.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="ACHJZ2_003178"
     CDS             complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="ACHJZ2_003178"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO4569248.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="ACHJZ2_003179"
     CDS             complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="ACHJZ2_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO4569249.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="ACHJZ2_003180"
     CDS             complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="ACHJZ2_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO4569250.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="ACHJZ2_003181"
     CDS             complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="ACHJZ2_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO4569251.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16988..17968
                     /gene="yggR"
                     /locus_tag="ACHJZ2_003182"
     CDS             16988..17968
                     /gene="yggR"
                     /locus_tag="ACHJZ2_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO4569252.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="ACHJZ2_003183"
     CDS             complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="ACHJZ2_003183"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO4569253.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="ACHJZ2_003184"
     CDS             complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="ACHJZ2_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO4569254.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="ACHJZ2_003185"
     CDS             complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="ACHJZ2_003185"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO4569255.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="ACHJZ2_003186"
     CDS             complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="ACHJZ2_003186"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO4569256.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21021..21728)
                     /gene="endA"
                     /locus_tag="ACHJZ2_003187"
     CDS             complement(21021..21728)
                     /gene="endA"
                     /locus_tag="ACHJZ2_003187"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO4569257.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="ACHJZ2_003188"
     CDS             complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="ACHJZ2_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO4569258.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22397..23791)
                     /gene="galP"
                     /locus_tag="ACHJZ2_003189"
     CDS             complement(22397..23791)
                     /gene="galP"
                     /locus_tag="ACHJZ2_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO4569259.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         5899 a         6015 c         6268 g         5756 t
ORIGIN      
        1 ctaataccgt tttgtctgtt tttttaacaa agaaaataat cctggagtag gttcccgggc
       61 atttgcccgg gatgattact gttttaatgg attattaatc tttgcatatt caaatggact
      121 gataaatctt tctctgtttg catccagaaa atcggcccac cactgtagca tcagtcgccg
      181 ttcttccaga tgttctgctt tatgaatgta agcagcacgt acaccgttac gttcctgatg
      241 gctcatctga cgttcaactg catcgcggga ccacaaacct gattctatta acgcactaca
      301 tgccatcgcc cggaatccat gaccacagac atcctgggtt gtgtcatacc ccataaccct
      361 cagggcttta tttacggtgt tttcactgat tggtttacgt gggtcatgtg ctcccgtaaa
      421 gatcagacca ttttcaccag cccatgtctg aagttctgcc agtatggcta caacctgttt
      481 gctgagtggc acaagatgtg gtgttcgcat cttggatccc cggtgtgaga attttacgcc
      541 tggcagaggt tcgcgttcgg ctggaatagt ccacatggca ttgtcaatat caatttcaga
      601 ccagcgggcg tatctgagtt cactggaacg aataaaaacc agtaaattca gcataacggc
      661 aagctgggtg acaggttgcc ccttgtaact gccaatacgc gacaaaagtt caggcaggcg
      721 agaaagatca agagcggggc gatgctggcg ttttaccgta gtcaatgcgc cagccatatc
      781 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cggcagatat
      841 tctttgctgc agacgactgg cgatttcata aatttgttta gcttctgcag cacgaacagg
      901 gataagcagg tccggggtat tgagtgttgt gatatcccga ttgccgataa aggggaatac
      961 gtgtgtctcg aggcttttga gtactttatc gctgtgtgat tgtgcccatt gtttattact
     1021 ggcatgccag cgtcgtgcaa tatgttcgaa agagagttgt aaatcaggga ctactttttt
     1081 agctcttttc tgttcgcaag ggtcaacccc ctccgaaatc agttttcggg cctcatcccg
     1141 ttttgtacga gcttcggaga gagatacttc tggatacaca acaagtgcca gagtcttctc
     1201 tttacccaga atacgataac ggagacgcca gtaacgggaa ccattaggat gtaccagcag
     1261 gaacatgcca gccccgtcag tgagtttata agccttgtca gtgggctttg cagcccggat
     1321 ttttgcgtca gtcagtgcca tagttcagcc ttctgaatgc gttaaagagg gtacaaattc
     1381 tgtcgaactg gggtatgccc gcaattatac ccacacaatg aggttgatgt ggggagaatt
     1441 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     1501 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga
     1561 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     1621 gcgcattaaa cctgattcgc ctcgtctcgt caatcaaatt tcttcaattt actgcagact
     1681 gcacaatcta tcatcacttt gccgctattg cacacattgt caggcaaacc aggcggtcca
     1741 ggcggcggaa agcggcattg ccagaaggag tgctggaagc atgttgacca ccgggaacat
     1801 tttaatgcca cagatgcgta atccggttgc cagcagcaat aaaccgccta cggcgctgaa
     1861 gtctgccatc atcgacggtg tggtcagcgg taatatcagc gcggcagccc acgccagcgt
     1921 taactggatg atcagtaatg ggatactaat caccgatacc gcaataccca gcgagcaggc
     1981 gaagatcatc gccgtaaaga aatcaagaaa tgacttggcg attaaaatac tcggatcgcc
     2041 ggtcatccct tcgttcatcg ccccgaagat cccggtgccg ctggcgcaaa acaggacaat
     2101 aatcgcgaca taattctgaa taaaagattc atgcgctggc ttcttacgtg agtgacgaaa
     2161 cagattttgt gctttggcga ccgctgtatt gacgcctttc tccagcagac aaatttcgcc
     2221 gattaatgct ccgagtaagg ttgctaaaac catcgccgga aggttggcgc atttcaccac
     2281 cagtaaaata ccaatcccca gcgatgccaa accaaaaatt gatgtcatgg agacgcggat
     2341 acgttccggt aagcgttggc taagcagtgc accgagcatg ccacccagta atattgcact
     2401 tgcgttgata aaagggccga tgaccacaag agttcctgtt aactgtattg tttgattttc
     2461 ttattatgaa ctttttgcct tgttgatggc ttgtctggtc gcaaagtcct ggcttgcacg
     2521 ccttagcgaa aggtgccatg attgcgcgaa ttttctcctc tctgtacgga gtttgcccga
     2581 tgcacgccat ctccttacat tctctcgctt atcgccgttt cgcgcgaaac ccttctcttt
     2641 ttatgctact gctcatgcgg tgaagttaac gcacgctcac tgcaggacaa cagtaaaatc
     2701 agagcctttc tgcttttact gatgtctggc ggtcggagct ggtgaccagt ttgacccata
     2761 tctcatgggg cagggtttta caccttgtcc agtattctta cttccccgaa acgggtttgc
     2821 gcttatgaaa tcaatgaata ttgccgccag tagtgaactg gtatcccgac tttctactca
     2881 tcgccgcgtg gtggcgttgg gagatactga ttttacggac gtcgcggcag tcgtcattac
     2941 cgctgcggat agtcgcagtg gcattcttac gttgcttaag cgcaccggtt ttcatctacc
     3001 ggtgtttttg tattccgaac atgctgttga attacctgcg ggcgttacgg cggtaatcaa
     3061 cggcaacgag cagcagtggc tggagctgga atccgcagcc tgtcagtatg aagagaattt
     3121 gctgccaccg ttttatgaca cgctgacgca gtacgttgag atgggcaaca gcacctttgc
     3181 ttgccctgga catcaacatg gtgcgttctt taaaaagcat cctgccggac gccattttta
     3241 cgatttcttt ggtgagaaca tctttcgcgc cgatatgtgt aacgctgacg taaaattggg
     3301 cgatctgctt attcatgaag gatcggcgaa agatgcgcag aaattcgcag ccaaagtctt
     3361 tcatgccgat aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata aagtggtgac
     3421 gaatgcgctg ttaacgcgtg gcgatctggt gctctttgac cgtaacaacc ataagtcgaa
     3481 tcatcacggc gcgttgattc aggcgggggc gacgccggtc tatctggaag cttcacgtaa
     3541 cccgtttggt ttcattggcg gtattgatgc gcactgtttt aatgaagagt atctgcgcca
     3601 gcaaattcgc gacgttgcgc cagaaaaagc cgacctgccg cgcccgtttc gcctggcgat
     3661 tattcagctg ggaacctatg acggcactgt ctataacgcc cgtcaggtga tcgataccgt
     3721 tgggcatctg tgtgattaca ttctgtttga ttccgcgtgg gtcggttatg aacaatttat
     3781 cccgatgatg gcggatagct cgccgctgct gttagaactt aacgaaaacg atccggggat
     3841 ctttgtgact cagtcggtgc acaaacagca ggcgggattc tcacagacgt cgcagatcca
     3901 taaaaaagat aaccatatcc gcggacaggc gcgtttttgc ccgcataagc ggttgaataa
     3961 cgcctttatg ctccatgctt ctaccagccc tttctatccg ctgtttgctg cactggatgt
     4021 taacgccaaa attcatgaag gggagagtgg gcgtcggctg tgggctgagt gcgttgagtt
     4081 gggtattgag tcgcgcaagg ctattcttgc gcgctgtaag ctgttccgcc cgtttatccc
     4141 gcccgttgtt gatggcaaat tgtggcagga ttatccgaca tcagtgttag ccagcgaccg
     4201 tcgttttttc agttttgagc cgggggcgaa gtggcacggc tttgaaggat atgccgcgga
     4261 tcagtatttt gttgatccgt gcaagctgtt actcactaca ccaggtatcg atgccgaaac
     4321 cggcgaatat agcgactttg gcgttccggc gacgattctg gcgcactatc tgcgtgagaa
     4381 cggcattgtg ccggagaagt gcgatctcaa ttccattctg ttcttattaa ctccggcgga
     4441 aagccacgag aagctggcac aactggtggc gatgctggct caatttgaac agcatattga
     4501 ggatgactcg ccgctggctg aggtgttgcc gagcgtttat aacaaatatc cggtgcgcta
     4561 tcgcgactac accctgcgcc agttgtgtca ggagatgcac gatctgtatg tcagtttcga
     4621 cgtcaaagac ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag tggtgatgaa
     4681 cccccaggat gcgcatagcg cttatattcg cggtgacgtg gagttggtgc ggattcgtga
     4741 tgccgaaggg cgaattgcgg cagaaggggc gttgccttat ccacctggcg tgctttgcgt
     4801 ggtacccggg gaagtctggg gtggggcggt tcaacgttat ttccttgcac tggaagaagg
     4861 ggtgaatttg ttgccgggat tttcgccgga gctgcaaggt gtttatagcg aaaccgatgc
     4921 ggatggcatg aaacggttgt acggttatgt gttgaagtaa gaataaaaaa aacgggtcgc
     4981 cttctggcga cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt acctgtcggg
     5041 acacgaacgt gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggagtaa
     5101 ccagcgaaaa tcaaccatac ggtctgccag tcggtaatac cgttttgggt gtacatctca
     5161 acaactttac cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg
     5221 aacatccctt gtgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa
     5281 ccagagatgt tgaagaagtc gaacgcacag ccgtaaacaa tcatcgatag aaccagcagt
     5341 acggtgccga acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc
     5401 acaatactga tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc
     5461 agaatgaaca gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcaca
     5521 ataaagctgc tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac
     5581 atgttggtaa tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata
     5641 cgcttgtttt taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc
     5701 gcttgctgtt tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg
     5761 gaaagtgctg cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc
     5821 acccacattg ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta
     5881 acgatatcca tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc
     5941 gttggcatat aagcaaacga gttaatcaat atcacaagga acatcgcctc cggcgtcgtg
     6001 acctgtgccg ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat
     6061 ttcgcactta accatttgtc ggccacaatc cccagcagcg caggcataaa gaccgcggcg
     6121 atacccagtg agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac
     6181 atataggaac cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg
     6241 attttcagct gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt
     6301 ttgaaaaagc acatttgacc gtgcgagatg atgggattct atcaacttcg cggactggtt
     6361 ttttgttacc tgtggcaaat aattgcaaac attcctaatt tgcaaaacga aattgtgata
     6421 tggataacat ttttgcccct gagcatcgtc aggggcggtt agtggaatta tcggcggcgg
     6481 taggattttt gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg
     6541 cgggtcgtca gcgtttgata aacatcgccc ggcgtcatgg tgttaataat attagccgcc
     6601 tgaatcttat cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg
     6661 atgacggcat aacgccggga tgtagggtta tcaattccgc cgagataaac attgttcagc
     6721 atcgccagat acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg
     6781 ctcggcgtgc cggatttccc ctgcgagcgg aacacatctt tcccggcagt atgttgtacc
     6841 acctgcatta accccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca
     6901 gtctgcataa ttgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg
     6961 accatgccga gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg
     7021 acgctgtaga tgatacgcag cccgttgctg cggctcttca gacggttttt cagcagataa
     7081 tccgcgaagt tgcttgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc
     7141 tgaccgtaaa ggaaaggttc tttcgaaatt gtaatatcat caacgtcgga atagagatcg
     7201 accgaactcg gatcgtcacc catcagcaac gttttgataa ttgcccggcg caaatgcgcg
     7261 gcaggttctg tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg
     7321 ctgcgggtct gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc
     7381 tctttgaatc cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa
     7441 ccgttggtgt ctttgaccca ggcttcgtta taggtaccgc cttttttggt cgtcgaacag
     7501 gagatgagca acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg
     7561 tgttgtgttt gtttttatgc aagctgccgg aggcgtgctc cggcactgtt tttatttttt
     7621 atcttccggc gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat
     7681 ctcctgctcc agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat
     7741 cagcatggtt tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat
     7801 gcgttttccc agttcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag
     7861 gaaagtacaa aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac
     7921 cggcgcgcca gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga
     7981 cggcggttgc gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga
     8041 cgacacggga agccacatag gcacaatatc taagtggaaa tggctgaagg tatgccgaaa
     8101 cgcggtcagc tgcgtcaggt tatcggcagc aatctgccgt tgcgccagcc actgccgcaa
     8161 actttcttcg tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg
     8221 acgctgcgcc agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc
     8281 cggcagcgtc tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc
     8341 aatacatccg ttttgtagcg gacagagcga acatttcggc ttcgaacgcg tgcaaatcat
     8401 cgcgcccaaa tccatcatcg cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg
     8461 ctcgcttaaa ctccataatt tattctcgac ctcttttttc ccaggccagc cgcttacagc
     8521 atagcagcgc gccagcaccc gtttgacgtt accgtcgaga atcggaaagt gcttacccag
     8581 agaaagcgat agaatcgcgc ctgcggtgga acgcccgacg cccggcagtg ctgcaacttc
     8641 ctcaaaggtt tccgggaatt taccgccgtg taaggtcgcc acttgttgtg ccgctttatg
     8701 cagattgcgc gcgcgggcgt aatagccaag ccccgtccac aagtggagaa cttcgtcgag
     8761 cggcgcattg gcgagatcgg tcaccgtcgg gaagcgcgcc ataaagcgtt caaaataggg
     8821 gataacggtc gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg
     8881 cgtcttgtca atttgccagg gcagagtttt tcgcccgtat ttatcgtacc agtccagaac
     8941 ctgggctgaa aattgcgacg cttgcatggt caccgaattc actgttgttg ggggcaagat
     9001 tgcagcacag cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc
     9061 ttgcatccgg caactaactt tggataatgc ccgttttcag aacactttca caagcgacta
     9121 aatctttatg aaaaacgacg tcatttcacc ggaatttgat gaaaacggcc gcccactgcg
     9181 ccgtatccgt agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct
     9241 ggaaaactac tggccggtga tgggcgttga gttcagcgaa gatatgctgg atttccccgc
     9301 gctttttggc cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gggcgtcgct
     9361 ggtggcaatg gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc
     9421 gggcgttggt gcgtgcctgg cttctgcgca tgaagagggc ttaagcaacc tgcgcgtgat
     9481 gtgtcacgat gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt
     9541 gcagctcttt ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca
     9601 ggtgccgttt gccgaactgg taaaaagcaa actgcagctg gggggcgtat tccatatggc
     9661 gaccgactgg gaaccttatg cggaacatat gattgaagtg atgtcttcta ttgacggtta
     9721 taaaaacctg tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa
     9781 atttgaacaa cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt
     9841 gaaataatgg caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc
     9901 caggaattag gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt
     9961 gataaaaccg ttgatgattt tattaacgag gttatcgaac cgaacaaact ggcctttgac
    10021 ggcagcggtt atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc
    10081 gaagaacatc aggcgattgt gcgtaagtgg ctggaagagc gcaacctgga tgaggtacgc
    10141 accagcgaac ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca
    10201 aaactgcctg atgcgctacg cttatcatgc ctacaaagat gcacgatcga gtaggcggga
    10261 taaggcgttt actccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca
    10321 gcaaatgctg gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg
    10381 gcagcacttt cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg
    10441 cgtgacgatg tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg
    10501 gtgatcacgc cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc
    10561 cagcgcgagc aggcgaagga ttatcaggct gaactacgta gcacgctgcc gtggattgat
    10621 gaaggcgcga aaagccgcgt cgagaaagcc cgtattgctc tggataaaat tatcgttcag
    10681 gagatgggcg aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa
    10741 gagcagatga accgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc
    10801 attgatcagg ttcgcgctga aggccagcaa ttagtgaatc aggcaatggg cggaatttta
    10861 caggacagca ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta
    10921 cagaatgtgc tgggaagcct gggggggctg caatcctcaa tccaaaccga gtggaaaaag
    10981 caggaaaaag atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa
    11041 gatagccgca aagccctggt cgggaattta aaataatcct ctattttaag acggcataat
    11101 acttttttat gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca
    11161 aaatatattc acgttgtaaa ttgtttaacg tcaaatttcc catacagagc taagggataa
    11221 tgcgtagcgt tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc
    11281 cgcactggtt atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc
    11341 aaccggcggg accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg
    11401 taaagttggc gtagaaaatc tggttaatgc ggtgccgcaa ctgaaggaca ttgcgaacgt
    11461 taaaggcgag caggtagtga atatcggctc ccaggacatg aacgatgatg tctggctgac
    11521 actggcgaaa aaaattaaca ccgactgcga taaaaccgac ggcttcgtca ttactcacgg
    11581 taccgacacg atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc
    11641 ggtggtgatg gtcggcgcaa tgcgtccgtc cacgtctatg agcgcagacg gtccattcaa
    11701 cctgtataac gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt
    11761 ggtgatgaac gacaccgtac tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt
    11821 agcgaccttc aagtctgtta actacggtcc gctgggatac attcacaacg gtaagattga
    11881 ctaccaacgt accccggcac gtaagcacac cagcgacacg ccgttcgatg tctctaagct
    11941 gaatgaactg ccgaaagtcg gcattgttta taactacgct aacgcatccg atcttccggc
    12001 taaagccctg gtagatgcgg gctatgatgg catcgtgagc gctggtgtgg gtaacggcaa
    12061 cctgtataaa tccgtgtttg acacgctggc gaccgccgcg aaaaacggta ctgcagtcgt
    12121 gcgttcttcc cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa
    12181 atacggtttt attgcctctg gtacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcaact
    12241 ggctctgacg caaaccaaag atccgcagca gatccagcag atcttcaatc agtactaatc
    12301 atttcgcccc ggtatcgcgc cggggctttt tcacttcaga cttacgtcca ttgccaattt
    12361 taattgccct aatgataatc accagaataa attattccgc gcgagggttt tcgggtgaaa
    12421 aagcaatgga ttgttggcac ggcgctgctt atgttgatga ctggtaatgt ctgggcagat
    12481 ggcgaaccgc caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt
    12541 ctcaagcttg atgccatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg
    12601 tcagcggaag gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca
    12661 gattacgaac tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg
    12721 ttaaccagta aaggaacgcc cgcgtctggc tggacggtga acttttactc ttttcaggcg
    12781 gcagctagcg atcgtggtcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc
    12841 gtgaatagcg aagatttcaa ttatcgcttt agtcagcttg agactgcgtt gaatacccag
    12901 aagaactcga ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg
    12961 gcgcagaaag cagcggatgc atattgggga aaagatgcaa atggtaagca aatgacccgc
    13021 gaagatgcct ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc
    13081 gaggcgttcg cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat
    13141 aaacagagtg aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac
    13201 gaacaacggc gacagacttt tctgcaatca caaaaactta gccgtaaatt gcaggatgac
    13261 tggataacgc tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt
    13321 aaaaaagtcg cgattttgat gaaaattgac gatatcaacc aggctaatga acgctggaaa
    13381 aaagataccg aacaactgcg gcgcaatggg gtgattaagt aagttttgat atagcgcacg
    13441 atttgtagca ggatgcggcg ttgaccccgc atccggcaat caacgtctga tgcgacgcta
    13501 gcgcgtctta tcagacctac aacagctgcc acatgtcggc tgaggtgctc acgccgcatc
    13561 cggcaatcaa cgtctgatgc gacgcttgcg cgtcttatca gacctacaac cgttgccaca
    13621 tgtaggctgg ataaggcgtt cacgccgcat ccggcaatac aagtttactc agccagaaaa
    13681 agctccagca gcgaatttaa aaacagcttc ccatgttccg taatctgcca gtaatccgca
    13741 cattcggtga gataaccctg ggcaatcgcc tcgtctaact gtgggcgaat cacatcttcg
    13801 caaagcccgg tatacgcact aaactccacg cgcggcgcag gttccagcaa ccggaagcga
    13861 ttcataaaga actcaaacgg cttatctgcg gcttcgacat cacgctggct ttccagatac
    13921 cttccttgca taaaaccacg cggatgacgc gttttggtgg tacgcagaat gcgcccatcc
    13981 gggaaggtca ctttgccgtg cgcgccgcag ccaataccga tgtagtcacc aaagcgccag
    14041 tagttgagat tgtgctggca ctgataaccg ggtttggcgt aagcggaagt ttcatattgc
    14101 tgataacccg ctgcggttaa taactgatgc ccctgttcga aaatatccca cagcgcgtcg
    14161 tcgtccggca gtaccggcgg gcgtgaacca aacagcgtat taggttcgat ggtcagttga
    14221 taccaggaaa gatgcggcgg attcagttca atagcctggc gcagatcgcc aagcgcctct
    14281 tccagcgatt gatccggtag cccatgcatc aaatcaaggt taaagctacg taaccctaaa
    14341 ccgctcgcca gctttgccgc tcgtttcgct tcttgcgggc catgaatacg tcccagccgt
    14401 ttcagctttt cttcgctaaa actctgtacc ccaatagaga tacgatttac accggcacgc
    14461 tgataatcaa caaagcgatc ggcttctacc gtaccggggt tcgcttccat ggtaatttct
    14521 gcatccgctg ccagcggcaa acgcgcacgc acgccgtcca gcagcgtttg catcgccgag
    14581 ccggaaagca ggctcggcgt accaccgcca ataaagattg tctttacttc acggccctga
    14641 gcgtaagcca catcgttgtc cagatcgtta agcagatgct gaacgtaatc gtcgtgcggc
    14701 acttctcctt tcaacgcgtg agagttgaaa tcgcagtacg ggcatttctg cacgcaccac
    14761 gggatgtgaa tgtagagact cagcggcggt aatttaacca ttacgtaaag cgtccagcag
    14821 cagtttcaat gcctgaccac ggtgggaaat ggcgctcttt tcttcgcggg tcagttcggc
    14881 agcggttttc ccttcggaag gtacgaagaa gattggatca taaccaaagc caccagtgcc
    14941 cgccggttcg cgagtaatca cacccggcca gctgccatgg cacaccagcg gggtcggatc
    15001 ttctgcgtga cgcagataca ccagcacgca gtggaaacgc gcctgacgtt ggtcgtccgg
    15061 tacgtctttc agtgtttcca gcagtttttg cagattcttt tgatcggtcg cgtcttcacc
    15121 ggaataacgt gcggagtaaa tacccggcgc gccgccaagc acatctaccg ccaggccaga
    15181 gtcgtcggca attgccggta aaccggtcac ttttgccgcg tggcgcgctt tcagaatcgc
    15241 gttttcgata aaggtcaggc cggtttcttc agcggaatca acgccgagtt ctgtttgggc
    15301 cacgatatca agaccgaagt cgctaagcag cgacgccagc tcacgcactt taccggcatt
    15361 gccggttgcg aggacaactt tttgcatagg atacctaatt aattaacgcc gcgacttctg
    15421 gcgggatttg ttgcggatta atgattttaa tttgtttgtg gcggccaagt tcgccttttt
    15481 caatcaccac ctggcttttg gcaacccgga attgcttacc gagaaacttc accagatggc
    15541 tgttggcctg accgtcaacc ggcggcgcgg taatggcgac ttttacctcg tcgccatgta
    15601 aaccgacaat agaatcacgg ctggctttcg gctgaatata gagccgtaaa accagaccgt
    15661 catcgttaac tgttacggca ctcataacgc catccacagc cccggcagca gcatatttcc
    15721 ggtcgcctgt aatacttctg cgacacccat attgatgaca tacagcagca gaacgaggat
    15781 catcggcgag aaatcaatcc cccccattgc cggtagcaag cggcgaatcg ggcgcagcag
    15841 cggatcggcc agctgaatca gcacgtattc aatcgggcta cggccctggc ttacccagct
    15901 cataatcgcc atcaccagca ggacccagaa aatcagcagg ccgatggttt tcagcagaat
    15961 cagtaaaccg gcaatccaga tgattggcag gaaggtcacc actttaaaca gcacgatggc
    16021 tttgataaaa ctgagaatat aggcaaccag cagcgaggcg ctatcaatgg gtcccattgc
    16081 cggaataacg cggcgcagtg gcccgataat tggctgcgtt accttcacta caaactgtga
    16141 gaaggggttg taaaaatcac aatgagccca ctgcatccag atgcgtaata acagcaccat
    16201 ggtatacagc tcaatgaccg ttgaaagcag gaaagtcaac gtattcatgg cgttccttta
    16261 attccttatt ttttagagta atcacgcgca ccaaaaattg cagtgccgat acgaaccatc
    16321 gtgctacctg ccgcaatggc ggcttccata tcgtccgaca ttcccagaga gagcgtgtcg
    16381 atatgcgggt agcgcgtttt cagtccggca aatgctacag ccatttggcg tgcaacttca
    16441 aactgcctta catattctga ctcaggcgca gggattgcca tcagcccgcg cagacgtaaa
    16501 cgcggtagtt cagcgaccgc agctgccagc tcgtccagtt ctgccagttg aatcccggac
    16561 ttactgtttt catcactaat attaatttga atcagtacgt taagaggggg aagttctgcc
    16621 gggcgctgat cgttgaggcg ggtagcaatg cgcaaacggt cgatggtatg acaccagtcg
    16681 aaatgctctg ccaccaggcg gcttttatta gactgcaacg ggccaataaa atgccattct
    16741 aatcctgtta cgcccagttc ctgaaagtgg cgaattttat ctaccccttc ctgaacgtag
    16801 ttttcaccaa attgacgctg cccggcatca atggcttctg cgatggcgct cgcaggtttt
    16861 gttttactga ctgcaagcag cgtaatttct tctggagaac ggccgcaacg cgttgcagcc
    16921 gctgagattt tgtcccggac ctgtgccagg ttatgcgcaa tatcgttcat tttccgagga
    16981 tcttagtatg aatatggaag aaattgtggc ccttagtgta aagcataacg tctcggatct
    17041 acacctgtgc agcgcctggc ccgcacgatg gcgcattcgc gggctaatgg aagctgcgcc
    17101 gtttgatgcg ccggacgtcg aagagctact gcgggagtgg ctggatgacg atcagcgggc
    17161 aatattgctg gagaatggtc agctggattt tgccgtgtcg ctgacggaaa accagcgatt
    17221 gcgtggcagc gcattcgcgc aacggcatgg catttcgctg gcgttacggc tgttaccttc
    17281 gcactgcccg cagctcgaac agcttggcac accaccgata ttgccggagt tactcaagag
    17341 cgagaatggc ttgattctgg tgacgggggc gacggggagt ggcaaatcta ccacgctggc
    17401 ggcgatggtt ggttatctca atcaacatgc tgatgcgcat attctgacgc tggaagatcc
    17461 tgtggaatat ctctatgcca gccagcgatg tttgatccag cagcgggaaa tcggtttgca
    17521 ttgtatgacg ttcgcatcgg gattgcgggc cgcattgcgg gaagatcctg atgtgatttt
    17581 gctcggagag ctgcgtgaca gcgagacaat ccgtctggcg ctgacggcgg cagaaaccgg
    17641 gcatctggtg ctggcaacct tacatacacg tggtgccgcg caggcagttg agcgacttgt
    17701 ggattcattt ccggcgcagg aaaaagaccc cgtgcgtaat caactggcag gtagtttacg
    17761 ggcagtgctg tcacaaaagc tggaagtgga taaacaggaa ggacgcgtgg cgctatttga
    17821 attgctgatt aacacacccg cggtggggaa tttgattcgc gaagggaaaa cccaccagtt
    17881 accgcatgtt attcaaactg ggcaacaggt ggggatgata acgtttcagc agagttatca
    17941 gcagcgggtg ggggaagggc gtttgtgacg ttgccatgcc cgatgcgcaa gcttatcggg
    18001 cctacaaagg caataaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgc
    18061 tgccacatgt aggccggata aggcgttcac gccgcatccg gcaataaggc gtaaggcgcg
    18121 atgatattat ttacactcca gcaggcgatt aaatctcctt aatatccctg ctcgaaatag
    18181 ctttcgagaa taataaccgc agaggcagag tcgattttgc ctttgttgag cgcccgatag
    18241 ccgccctgtt caaacagacc ggaacgtgcc tccacggtgc taagacgctc gtcatggagc
    18301 tttacttcaa caccgaaacg accatgaata cggttagcaa atttacgcgc tctggcggtt
    18361 aacggctgtt cggtgccgtc catattcagc ggtaaaccga cgatgatttc gtctggctgc
    18421 cactctttca gtaaacgttc gatgaggttc cagtccggcg taccgtcctg tgctttaatt
    18481 gcaggcaaag ggcgagcggt gccggtaatg cgttggccga ccgctacgcc aatgcttttg
    18541 gtgccgaagt cgaaggcgag taaggttcca ctcatcaggc gtgtcctgcc acaccaggca
    18601 tggtgagaat atccacacca atcagttttg ccgcctcgcg ccagcggtcg gcaattggcg
    18661 ttttgaacag aatattcaga tctgccgggg cggttagcca cgcgttatcg agaatttctt
    18721 gttccagttg acctttttcc caggaggcat agcccagagc caccaatacg tcagacggtt
    18781 gtttatcggt gccgagcgtt tccagcacat cgcgggaagt ggtcatcact gtgttgtctg
    18841 aaatgcgaat gctggaagca aaattggagg gcggagtatg caaaataaac ccgcgatctt
    18901 cagccagtgg gccgccgagc ataaccggtt tatccagacg gattgattca tcacgcggct
    18961 ccggcgtgat cttcagcttt tccagaatcc cttcaatttt gagattttcc agcggcttgt
    19021 tgacgatgat ccccattgca ccattggtat tatgttcgca aatgtagacc acggaacgac
    19081 ggaaaatcgg atcctggaga gcaggcatgg caataagaaa gtgatgctgt aaattcattg
    19141 tcagaggttc tgtttcctgg ttcaaaaaag caacagcgcc cagtatgcgt ggaaagaagg
    19201 gcgctgtcac tcagagtctc aacgagatcc ttctcgctaa ggtgggttac tactgctgta
    19261 aacgtgcttc gatggcatcc attaacattc cggtgatcga caccggaaac tctgcttcaa
    19321 tctcacgaat acaggttggg ctggtgacgt taatttcagt cagacggtcg ccgatgatat
    19381 ccagaccaac aaaaatcagc cctttttctt tcagcgtcgg cccgatctga cgggcgattt
    19441 tccagtcact ttccgtcagc ggacgaggtt caccgcgacc accggcagcc agattgccac
    19501 gggtttcgcc cccctgcgga atacgcgcca ggcagtacgg tactggttcg ccgtccacca
    19561 ccagcacgcg tttgtcgcca tctttaatgg ctggcagata attttgtgcc atgcagtagc
    19621 gagtgccgtg ttcggtcaag gtttcggcaa tgacgccgag gtttggatcg ccttctttca
    19681 cgcggaaaat cgacgcgccg cccataccgt ccagcggctt aagaatgatg tcgctgtgtt
    19741 tctcccagaa cgcttttagc tgcgctttat tgcgcgtaac cagcgtttct ggcgttaagt
    19801 cagagaacca ggcggtaaac agtttctcgt tacagtcgcg caggctctgc ggcttgttaa
    19861 cgatcagcgt ccctttctct tcggcacgtt ccagaatata ggtcgcgtag ataaactcgg
    19921 tatcaaacgg cgggtcttta cgcatcagga tcacatcgag atcggccagc ggcagatcct
    19981 gttcaccgac gaatgaaaac cactcttcgt agttctgttt cacgttcagc gtgcgggtat
    20041 gggcgcgggc ttcgccattg atcagataca gatcgcccat ctccatatag tgaagttcgt
    20101 aaccacgacg ctgtgcttcc agcaacatag cgaaactgga atctttcttg atgttgatgt
    20161 ttgcgatggg gtccatcacg atgccgagct tgatcattat tcttctccgt tagcccaaat
    20221 cgccaaatcg tacttgtagc gcggtaatgg cggtgagcgc agttgtctct gtacgcaaaa
    20281 cgcgaggtcc caacaggata tcagtaaatt gatagcgggc agtcatggca atttcatctg
    20341 ccgataaacc gccttccggg ccaatcagca ggcggacgcg ttcaaccggt aaaggcaacg
    20401 tattgatgct gttactggcg cgcgggtgaa gattcagttt cagtccttca tcctgctctg
    20461 cacaccaggc ttccagatcc atcgctggac ggatttccgg cacccggtta cgaccacact
    20521 gctcacaggc agcaattgca atcttctgcc actgctggag cttcttgttc agacgttcac
    20581 tatccagttt aacgccgcag cgctcagaaa aaagtggcgt aatgaggctt acaccgagtt
    20641 cgatcgattt ctggatcgta aattccattt tttcaccacg cgacatcacc tgaccgaggt
    20701 gaatatgcag cggagattcg cgatcgtcga tctggccttc cagcaccttc acttccacgc
    20761 tttttttgct ggcgctggta atttcggcgt caaagacctg gttgctgccg tcaaacaatt
    20821 gcaacgcctg tcccggcccc atacgcagta cgcgcccaat atggttggcg gcatcttcgc
    20881 aaagcgcgat gtgagaatgg ctggtcagtg gttcaggatg ataaatgcgg gggatacgca
    20941 tagttaaaaa tccgcgtcgt ctccccacgc ggttgtacgc gtggggtagg ggttaacaaa
    21001 aagaatcccg ctagtgtagg ttagctcttt cgcgcctggc aagcgcgttg cacatacggg
    21061 ttatgattgc cctgtacctt cgcgatgcgt tcatcgcgct cgcactccca gtcggtaacc
    21121 ggatacatct tgtcccatgc gttgaacagc tgcgtttgct ggcgagagag tgtcaggttg
    21181 taatggtcac gcatatagaa gtaggtgcgc gcaatggcac cgcgtgcacg cgctggtggt
    21241 tcggcagctt tttctttgaa atcgaccttc atggcgcatt gaccgtactg gccttcaccg
    21301 ccattccact ggctgtacat aaagttgccg cgatcgccat tcacctcgcc gactgacggt
    21361 tgcaggttat gcatatcgct ttccatcttg cgatagaccg gatctttggc gcaattttta
    21421 cgtccaccgt cctgccagca ctggcgctgg tgaccgaatt gccaggcggg aacgacgtgt
    21481 tcccactcta cgcggctggc gcggttttca tttttgcgca cctgatagcc gcacgattgc
    21541 agatcgacaa cgcctttttt gccctgccag ttaattttac atccgcaata aaacgtaccg
    21601 ggcgcgtcag cgtggacttt taccgccgcg gctttcgcct gagaaaaact attgatacct
    21661 tcggccaacg ccgggccgga aaatgctgcg ctcagtacca ccgcagcaat agacaaataa
    21721 cggtacatca cttactccgt gttaaaacga gccagcaacg tagcgaaagt tgttttggta
    21781 tgcaatcagc cagatcagga aagttcctga tagttcagat ggttattttg cgaccagttg
    21841 ttcgccgcag tgaacacagc gatagacggc ttcgccacga actacgcgat tatgacggcg
    21901 tacggtaagc tgatgttcct gacacttaca gctgtagggg aaggtgttgc ggcgcacgga
    21961 ttgcagttcg aactgatgcg tacggcgggc tggaacgccc agcacacttt ccatcatcca
    22021 cttccactct ttgccatgtg gtgctacgcg gccaaaatgt ttccatacca gcaaatgtgc
    22081 cagttcgtgc ggtaccactt cttcaataaa ggcttcactg ttttccaaca gcaaaacggg
    22141 attaaggcga atttcatagc tttccagcca ggctgttccg gcagaggttc cgcgctgggt
    22201 gtaagagagt tttggctccg ggtagttacg ccccaatttc aggttggcct gggcgagttt
    22261 ttcccgcagg cgacgcataa cggcctgctg gatggcgata gggagacggg atgttttcat
    22321 agcggcagag gatagagcga agaaaagatg actgcaagag gtggcttcct ccacgacggg
    22381 aggaagcctg gggagattaa tcgtgagcgc cgatttcgcg cagtttacga cctttcatca
    22441 gattacgttc aatatgttcc agcgaaacgt gtttggtttc tggcaccagc cacagtgtca
    22501 gcaggataaa caggacgttc agacccgcgt aaacccagaa ggtattagcg ttgcccagcg
    22561 tgttgagcat ggtcaggaac gttgcgccaa cgatcatgtt ggcaatccag ttggtggcgg
    22621 tggagcaggt gatgccaaaa tcgcggcctt tcagcggctg aatttcggag cacagtaccc
    22681 aaatcagcgg accggcactc atggcaaaac cgacaataaa catcagcagc atggcgatgg
    22741 cgaaatactg cgccgacgga gagtggatac cgatatgcat cattgtaccg agtacgccca
    22801 taccagcagc catcaccagg aagcccagcg ttagcgttgg tttacgtccc cagcggtcaa
    22861 caaggccgat tgcgataaag gtggcaagta cgttggtcag gccgacaatc actgtccccc
    22921 acatttgctc ggtggtgttg gtataacccg ccagttcgaa gattttcggc gcgtaataca
    22981 tgatgacgtt catcccggtg aattgctgca ttacctgtaa cagtacgcca aggaacaccg
    23041 cgcggcggaa gttgctattc tctttaaaca gcgcccagcc actctgttta acctgcaaac
    23101 tttcacggat ttcatccagt tcgcgtttcg cttccgcgct ggtgtcacgc aggcgtagca
    23161 gcacgcgttc ggcatcaaca aaacggcgtt tggcggcaaa ccaacgtggg ctgtctggca
    23221 ggaagaagac accaatcagc agcaaaattg ccgggatgat aatcacaccc agcatccagc
    23281 gccatgcacc ggtgtagctg aaggcggtat cagaaagata agcaccgagg atcccgattg
    23341 tgatcattaa ctgatacatc gagatcatac tgccgcgaat tttttccggc gcaatttcag
    23401 agaggtacag tggtgcggta taagaggcca cacctaccgc cagccccagc agaacgcggg
    23461 aaagaatcag tacttcaacg tttggcgcag ccgcagagaa cagcgaaccg gcaacaaaca
    23521 aaattgcgcc gatcatcagg ctctttttgc gcccgagttt aaaggagagc cagccgctgc
    23581 ccaccgcacc gactgccgca ccgaacatca tggagcttac gacccattct tgcgtgtgcg
    23641 aagtaatctg gaattcatct gcaataaacg gcagtgcgcc agcaattaca ccgatatcca
    23701 ggccaaagag taatcccgcc agagcggcaa ggaagcagac gaaaaacgtc attgccttgt
    23761 ttgactgccc ctgtttttta gcgtcaggca tgatgccctc caatatggtt attttttatt
    23821 gtgaattaag ataggtgagt acgacgtaaa aagatgtgaa gcaaatcaca tcagtgtaat
    23881 cgcttacact aatatttatc ttagttatga tttaagtgca tgaaaataat tgagttat
//
LOCUS       DAZFBR010000076        23910 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFBR010000076 DAZFBR010000000
VERSION     DAZFBR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.076"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(423..641)
                     /locus_tag="ACHJZ2_003190"
     CDS             complement(423..641)
                     /locus_tag="ACHJZ2_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569260.1"
                     /translation="MFKYAEMLMAEEDDKVCELIFKQQDNLILKINVRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLIVCTLGVNS"
     gene            complement(1973..2155)
                     /locus_tag="ACHJZ2_003191"
     CDS             complement(1973..2155)
                     /locus_tag="ACHJZ2_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569261.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            2094..4184
                     /gene="iha"
                     /locus_tag="ACHJZ2_003192"
     CDS             2094..4184
                     /gene="iha"
                     /locus_tag="ACHJZ2_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO4569262.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(4402..4561)
                     /locus_tag="ACHJZ2_003193"
                     /pseudo
     CDS             complement(4402..4561)
                     /locus_tag="ACHJZ2_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(4764..5084)
                     /locus_tag="ACHJZ2_003194"
                     /pseudo
     CDS             complement(4764..5084)
                     /locus_tag="ACHJZ2_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(5507..6082)
                     /locus_tag="ACHJZ2_003195"
     CDS             complement(5507..6082)
                     /locus_tag="ACHJZ2_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4569263.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(6151..6729)
                     /gene="terD"
                     /locus_tag="ACHJZ2_003196"
     CDS             complement(6151..6729)
                     /gene="terD"
                     /locus_tag="ACHJZ2_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO4569264.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(6778..7818)
                     /gene="terC"
                     /locus_tag="ACHJZ2_003197"
     CDS             complement(6778..7818)
                     /gene="terC"
                     /locus_tag="ACHJZ2_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO4569265.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(7841..8296)
                     /locus_tag="ACHJZ2_003198"
     CDS             complement(7841..8296)
                     /locus_tag="ACHJZ2_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO4569266.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(8319..9476)
                     /locus_tag="ACHJZ2_003199"
     CDS             complement(8319..9476)
                     /locus_tag="ACHJZ2_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4569267.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(9476..10057)
                     /locus_tag="ACHJZ2_003200"
     CDS             complement(9476..10057)
                     /locus_tag="ACHJZ2_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4569268.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            10380..11438
                     /locus_tag="ACHJZ2_003201"
     CDS             10380..11438
                     /locus_tag="ACHJZ2_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO4569269.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            11448..12590
                     /locus_tag="ACHJZ2_003202"
     CDS             11448..12590
                     /locus_tag="ACHJZ2_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO4569270.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            12583..13356
                     /locus_tag="ACHJZ2_003203"
     CDS             12583..13356
                     /locus_tag="ACHJZ2_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569271.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            13358..14437
                     /locus_tag="ACHJZ2_003204"
     CDS             13358..14437
                     /locus_tag="ACHJZ2_003204"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO4569272.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            14437..15393
                     /locus_tag="ACHJZ2_003205"
     CDS             14437..15393
                     /locus_tag="ACHJZ2_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO4569273.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            15404..16612
                     /locus_tag="ACHJZ2_003206"
     CDS             15404..16612
                     /locus_tag="ACHJZ2_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO4569274.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            16630..17097
                     /gene="terW"
                     /locus_tag="ACHJZ2_003207"
     CDS             16630..17097
                     /gene="terW"
                     /locus_tag="ACHJZ2_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO4569275.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            17358..17687
                     /locus_tag="ACHJZ2_003208"
     CDS             17358..17687
                     /locus_tag="ACHJZ2_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4569276.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            17674..18015
                     /gene="tnpB"
                     /locus_tag="ACHJZ2_003209"
     CDS             17674..18015
                     /gene="tnpB"
                     /locus_tag="ACHJZ2_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4569277.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            18097..18927
                     /locus_tag="ACHJZ2_003210"
                     /pseudo
     CDS             18097..18927
                     /locus_tag="ACHJZ2_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(18958..20571)
                     /locus_tag="ACHJZ2_003211"
     CDS             complement(18958..20571)
                     /locus_tag="ACHJZ2_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO4569278.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(20602..20952)
                     /gene="tnpB"
                     /locus_tag="ACHJZ2_003212"
     CDS             complement(20602..20952)
                     /gene="tnpB"
                     /locus_tag="ACHJZ2_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4569279.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(20949..21374)
                     /locus_tag="ACHJZ2_003213"
     CDS             complement(20949..21374)
                     /locus_tag="ACHJZ2_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4569280.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            21428..22179
                     /locus_tag="ACHJZ2_003214"
                     /pseudo
     CDS             21428..22179
                     /locus_tag="ACHJZ2_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            23712..>23910
                     /locus_tag="ACHJZ2_003215"
     CDS             23712..>23910
                     /locus_tag="ACHJZ2_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569281.1"
                     /translation="MEFSVIFFWVITGLITILFLKSIITDLIKNLNKTDIALYILAFL
                     SAGYTFQYIYNDNIFSELWGVG"
BASE COUNT         6089 a         6042 c         5943 g         5836 t
ORIGIN      
        1 tatattctca aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta
       61 aaaagtaaga attgataaaa aaacgccagc aacacatagt gccggcggag ggaatacccc
      121 atggagaaaa tgtgatgcct agagcatcat gaatatataa ttaaaaatag ttagcgttgt
      181 caatactttc acaaaaataa ttttcgtagt atagaaagat atttttatgc atgacctacc
      241 tgaatttgct ccgggtagag gttataaata aaaattgaat cacgacaaac acaatattca
      301 tagtatggcg atgcctacgc cagcaagaat agcgccaata atattgggaa tataatagac
      361 accagacgca caggcatctc actccttaac aaacaacaat caggatattt acttttacca
      421 agctaactgt ttacacccaa agtacacaca attaaccatt caataacaaa tggcaatatc
      481 catagccata cgactttacc ttgtaatgtt cggtatttct ttataattat tctgggaaat
      541 ctaacattta tttttaaaat caaattatct tgttgtttaa aaataagttc acatacttta
      601 tcatcttctt ccgccatcaa catctctgca tacttaaaca tttcagaacg ttcctttagc
      661 acagaagagt aattatatgt ccagttccca ggcaataatg cttatggata tttaattcat
      721 aatttagaga atattttgca ataatatttg gcagtattca gaaatacctg aaaaatcata
      781 ctatacagcc ctagggaatg gataaagatt ctaacaaagc atttcaacaa tatatacttg
      841 ttaaaaatcc atatcgaaat gccgttgcag cattaatata tgcctcattc ataaaatgta
      901 aaagagcaag ctcgtaccag taggggggaa attcattgac atgtcctgtc aatacgtaca
      961 gagccctgcc atgcttgcca gcacgtaaca attccgcccc cagtaaccag cgtgttccct
     1021 gattattctc agggttatat gttagtattt tatcaatgag tataacggca tcctgatgac
     1081 gctgtaaatg aacattcgcc agaattgcag cataaagagc gcaaatgcat atgccagatg
     1141 agcgtgaata tcaatgatgt caggagcatg tcaggcaaga cgcctgagtt tgttgacgta
     1201 atttgtttcg tttatttcat cactatcgta tgcgtcaaga gttttttcaa actcatcccg
     1261 aaatgggcca agcctggttt caggaaataa gaaataccct tggttattgt tcacttaaaa
     1321 gtataatttg taattcaaat cctgagcctg taaagcgggg agtaacatat tctattctga
     1381 agagaataaa agtcgtgatg cgatgtatca agcccggatt gtaatcccag attaacatag
     1441 atcacaaaac aacttattct tcactaacgt caagataaat atcgttgtat gccttatcac
     1501 gactacgaat accagcaaga tacaactgac atacggaaag ataccacgtt ttttactccc
     1561 gaaaataacg ctaaaaagct acttccccat cgtttgtcct tagtattgcc agcgccaaca
     1621 atgtgggctg acatgataaa gctgtctagg aaattgttcg cctcctcagc ggacaatcca
     1681 aatggtgatt gtctctgtta aacgtttatt ttgaaggtcg actgaataag gtgatgacgc
     1741 tgtagaattt ttcacgtgcc acagaatttt gaacgctttc tcttacaata tttcaatgtt
     1801 tctatcagta ttcgccggaa gaagtcatcg accaaatcat cccagtcgtc tcgcatcact
     1861 gaccattcat ggttgacatg tggtggaaat ccgcttctac agtaaccatt ttttattcgc
     1921 aaaaccgaac acgccatacg gatagctgtt aactggcatg ccgaggcagt cgttatttat
     1981 atttggtttt gtcaataatc tttatttttt gtaaaaggca aatataaatt attctcatta
     2041 ttgtttgtat ttgtgtattg tcttgccggt taacatgatc ggagattagt aatatgcgaa
     2101 taaccactct ggcttccgta gtcattccct gtctcggatt ttcagccagc agcatagctg
     2161 ctgcagagga tgtgatgatt gtctcggcat ccggctatga gaaaaagctg actaacgcag
     2221 ccgccagtgt ttctgtgatt agccaggagg aattgcagtc cagccagtac cacgatctgg
     2281 cggaggctct gagatcagta gagggtgtgg atgttgaaag tggtacgggt aaaaccggag
     2341 ggctggaaat cagcatccga ggaatgccag ccagttacac gctgatactg attgatggtg
     2401 ttcgtcaggg cggaagcagt gacgtgactc ccaacggttt ttctgccatg aataccgggt
     2461 tcatgccccc tctggccgcc attgagcgta ttgaggttat cagggggccg atgtccacac
     2521 tgtatggctc tgatgcgatg ggcggtgtgg tgaatatcat taccagaaag aatgcagaca
     2581 aatggctctc ttccgtcaat gcagggctga atctgcagga aagcaacaaa tggggtaaca
     2641 gcagccagtt taatttctgg agcagtggtc cccttgtgga tgattctgtc agcctgcagg
     2701 tacgcggtag cacacaacag cgtcagggtt catcggtcac atcactgagc gatacagcag
     2761 ccacgcgtat tccttatccc acggagtcac agaattataa tcttggtgca cgtcttgact
     2821 ggaaggcgtc ggagcaggat gtgctctggt ttgatatgga taccacccgg cagcgttatg
     2881 ataaccggga tgggcaactg gggagtctga cggggggata tgaccggacc ctgcgctatg
     2941 agcgaaacaa aatttcagct ggctatgatc atactttcac cttcggaaca tggaaatcgt
     3001 atctgaactg gaacgagaca gaaaataaag gtcgtgagct tgtacgcagt gtactgaagc
     3061 gcgacaaatg ggggcttgcc ggtcagccgc gggagcttaa ggaatcgaac cttatcctga
     3121 attcattact gcttacccct ctgggagaat ctcatctggt tacggtgggg ggcgagtttc
     3181 agagctcgtc catgaaagac ggagttgtcc ttgccagcac aggtgaaact ttccggcaga
     3241 aaagctggtc ggtatttgct gaggatgagt ggcatctcac ggatgcactt gcgctgactg
     3301 cgggcagccg ctatgaacat catgagcaat tcgggggaca cttcagtccg cgtgcatatc
     3361 tggtctggga tgtggcagat gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg
     3421 cacccagaat ggggcagcta cataaaggga ttagtggtgt gtccgggcag ggaaaaacaa
     3481 atctacttgg taaccccgac ctgaagccgg aagagagcgt cagttatgag gctggggtgt
     3541 attacgataa ccccgccggt ctgaatgcca atgtcacagg ttttatgact gacttctcca
     3601 acaagattgt ctcttattcc ataaatgata acaccaatag ctatgtaaac agcggaaagg
     3661 cccggttgca cggtgtggaa tttgccggca cattgccgct gtggtcagag gatgtcacgc
     3721 tgtcactgaa ttacacctgg acccgaagtg aacaacgtga tggtgataac aaaggtgcgc
     3781 cgctgagtta tacccctgaa cacatggtga atgcgaaact gaactggcag atcaccgaag
     3841 aggtggcatc atggctgggt gcccgttatc gcgggaaaac accacgtttc acccagaatt
     3901 attcgtcact gagcgctgta cagaagaaag tgtatgatga gaaaggagaa tacctgaaag
     3961 cctggacggt ggtggatgca ggtctgtcgt ggaagatgac ggatgccctg acgctgaatg
     4021 ctgcggtgaa taacctgctc aacaaggatt acagtgacgt gagcctgtac agtgccggta
     4081 agagtacgct gtatgccggt gattacttcc agacgggatc atcaacaaca ggatatgtga
     4141 tacctgagcg aaattactgg atgtcgctga actatcagtt ctgataataa caaaacgcta
     4201 tcactgacgg tagaatacgt tgccactgca actcctggcg gaacagtggc aacgtattag
     4261 gttaagtgca tttccgatcc gctaatgaga tttcgttacc aacaactaat atcgtcacag
     4321 gaaatgcacg gattattttt aacttatcat ttacatactt gtccagagtg taagcgcacc
     4381 gtgaaggacg tggggtaaaa attagtttac agagagagtg acgttccagg gcaacaactc
     4441 tttcatgcgg ttggcaggcc aggtgttgat tacactgatc acgtggcgta gccacgtttc
     4501 cggctcgatt ccgttaagtt ttgcagctac cgatcaggct gtacatcact gccgcactat
     4561 cgctcgtcat ctcaaagtcc ggtctcgtca gcaggaaggt atcattctct cccgccattt
     4621 ttccaggggc ccggtcagat aagtcccttt gtctatcgct gactcctgac tcataacccg
     4681 gttagcagaa tgcagggtca ccacctcgcc acgaccaaat ccaaataagt caattgcacc
     4741 ttctcaatcg ccattttgtc agtaagcgta cagcctgaac tgatggtatc ttcaccatca
     4801 atgacaacgg tgatcgcaat tttactgacg ttcgccggaa cacgatccag tgctatctca
     4861 atgctggcct gctgcgaacc ggtaacgagc ctgactgccc cctcaggaga agacaaatta
     4921 ttataaaaga taaagtcaga atcgccactg acctttccct gagcattaag catgaacagg
     4981 caggtatcgg gttcgccttt aaagccggat ttggcagggt actgaagatt cagcctgatc
     5041 gtagattgct gaaggggtat gttttgtccg gattgtaaat tcatattaac tctcctgatt
     5101 tttgattatt attaatgcgc agcgtttata tatgttccct aggcttagtt ctggacgctg
     5161 gatattcggt gaggcgtaaa tatggtatga caccattttt cataacgctg aagtttctat
     5221 acctgttgaa tttgaatttt cattgaccgg gtatcttatt ttccagggcc cgttccttca
     5281 taagtcgcaa aagtaacata tatccgaagg gcatgctgtt gatatcagac acggaatact
     5341 ggctttaacc agcaaccaca gataaacccg gggcctgcat aaaaggttca tgccagaatt
     5401 aacatagctc tgctttctgc cccccccctt tcccatattc acccgttgat agcggatcga
     5461 tacccaaaaa aaaccccggc attgccgggg tcagactcag cgtcttttag acattgacgc
     5521 cgtgctggga agcaagcgct gacagaccac cggcaaaacc ctggccaaca gctttaaact
     5581 tccactcagt accatggcga tacagttcac cgaagaccat tgcggtttcg gttgaggcgt
     5641 cttcagacag atcgaaacgg gcaatttccg tcccgttgtc gttgttgtaa acgcgcatga
     5701 agctgttgct caccatgccg aagttttgtt tacgcgcttc tgcatcatag atggtaacgg
     5761 caaataccag ttttttgatg tctgctgaga ctttggtcag atcgattttg acctgctcat
     5821 cgtcgccgtc gccttcaccg gtacggttgt cgccctggtg ctctactgcg ccatcagggc
     5881 tggttttatt attgaagaaa atgaaatggg catctgacag tactttaccg tcttcaccta
     5941 ctgcgaatac ggaagcgtcc agatcaaaac cctgaccatc ggttacacgg gcatcccagc
     6001 ccaggccaac catagcgaca ttcatggttg gtgcttcttt ggtcagagat acgttgccgc
     6061 cttttacgag agaaactgcc atttttagct cctgcaaaca gttgaatgag gctgaattaa
     6121 cagccccaga tatgaaaagt tacttttcga tcaggacgcg ttaatgccgt actgagcaca
     6181 tacagatgcc agaccaccag cataaccctg gcctactgcg cggaatttcc actcaccatt
     6241 gtggcgatac agctcgccga acagcatggc agtctcagtg gacgcatctt cggtcagatc
     6301 gtagcgagcg acttcagtct ggttatcgtc attaaccaga cgaataaacg caccggatac
     6361 ctgaccaaag ctctggcgac gagcctgagc atcgtggatg gtcacaacga agatgatctt
     6421 gtcaacttca gacgggacgg cgtccagttt aattttcagc gattcatcat caccatcgcc
     6481 ctcaccggtg cggttatcgc cggtgtgcgt tacggaaccg tcggatgacg tcaggttgtt
     6541 atagaagatg aaatctgaat cgccgcgcac tttgccgttt gaggccagca ggaatgctga
     6601 agcatccagg tcaaagtcct gaccgtctgt tgaacgcgca tcccagccaa ggcccaccag
     6661 gacatttttc attgacggag ctgctttact cagggagacg ttcccgcctt tggaaagaga
     6721 aacactcata aaataacctc ttcgattagt aattgttcag gttaacactt aaggggatta
     6781 actccccttt tcctcagatt caggtttgcc cgggaacatg acgcttgcga gaatgcccag
     6841 cgccagtaca cccagtacaa catacaggct ggttgttgcc gcgatgctgt aaccatgatg
     6901 ccagatgtga tcggtcgcat tcaggccgag ttttgccgcg atgaagaaca gcagcacgat
     6961 aacggccttc tccagatgaa ccaggtactg tttcagtgcc tcaaggacaa aatacagagt
     7021 acgcagaccc aggatagcaa acatcatggc actatagacg ataagcggtt cacgactgac
     7081 ggcaatgatt gccggtaccg agtcaaacgc gaacattacg tccgagagtt caaccacagc
     7141 cacacacagg aacagcgggg tcgcatacag cgccgctttt ttaccacggc caatggtgac
     7201 atcgctgttt tctggtttcg ccagttcagc atccacttcc ttctggttaa gtaggaaggc
     7261 atgccctctg agcttcggcc agatagggaa gaagcgttta accatacggt aagccagatg
     7321 ctgggagtaa tcctcaattt catcatcgtc atcaccgctt ttaagcatca tgaccgccgt
     7381 ccaggcaacg ataatagcga agacaacttc aacgtacggc cccagactca gcaggctcgt
     7441 accgatggcg acaaagatgc ccctgaagac gatggcacca atgatccccc agtagagaac
     7501 acggtggcga taacgatccg gaacggcgaa ccaggagaag attgccatca tgacgaacag
     7561 gttatcgacc gacagcactt tctccagcgc ataacccgtg acaaacagac tggcaacctc
     7621 agcaccgtgg tggatataga ggaaaccggc aaatgccatc gcaaccacaa cccagaatac
     7681 ggaccagagc gcagcactct tcagcgagat tggcttgtca tcacggtgca taaacaggtc
     7741 aataaagatg gcaccgactg aaagcgcaat aaaaacaatg accgtttcag tcgggaagcc
     7801 gatgtgtgtg gaaaccatat tttaccctta gggatgtgga tcagaggcca aattcagccg
     7861 ggtcgaagcc caacgcgata gcgatctcgc gggaggccag tttctcgtcc tgatcgaagt
     7921 taccgtcact tttcgcaacg gcaataccaa cacgtaaggc cagctgagcg gcctcaggct
     7981 gatctttcag cgccaggatg tatttcatgg tttcgccctt gccaatttca acatcgaaat
     8041 cgaagcttga aaccagttta ttgaagaact caatcacctc attggtatcg aagaccttca
     8101 gctcttctga agagcgcaga aagcccatca ttttctgctt ttcttccgaa cttacgccgt
     8161 cactcgatac ggcaatacgg gcacatacag caacggtgcc ctgcatgaat tttttgtttt
     8221 tgaaacggcc aacctggcgg gtcagttcgt cacggcctga gttaatggca cctttaactt
     8281 tgtcgaaaaa gctcatacct gtttcctttg ttgctgtgtt atttagaacc ggcgctccag
     8341 cgaaatcccc agccaaatgc ccggtccatt tcgtcctggc ctttgaagta ctggttaatt
     8401 cgctcgactt taattgcgcc gttttcgttt acgagtctgg caatggcgca caatgtgcga
     8461 cggttctcac cttcggtcag gcgggtctca atgggcggtt gatccggcac atgaatggtg
     8521 actacaccat cagtcttgtc ccagctggga acaccttcat aaataaaggc gtaaatcagc
     8581 acttcgcgga tatgcttcca ttcacgtcca ttgatatgca gccactcgcc gtctgataca
     8641 tcacccgtcc ggtcatcgcc cttgagctga acatacggtt catcgcgata atcaccgaat
     8701 gcattaccga gcgcctgaat gaccgattta tacccgtcct gaagctcaac aaaggccccc
     8761 agatccaggt cgattccttt ggagccgaac atacctgcaa agcctgattt gctactgccc
     8821 cggtgccagt tcaggttgat ccggatttca ccgaagttat cccgtttggt caggctgatt
     8881 gcaggcttct cttttgtcag cgataccttg ctcaggctga tttttgtctc aactggcgga
     8941 ggggttacaa ccggaggagt tggggctgcg ggagccggtt cgtcggcaat atttacgccg
     9001 aaatgctctg ccagcggttt aagtccaccg ttgaacccct gcgcaacgaa gcggaatttc
     9061 cagtcattat tacgacggta aaattccccc agaattaagg ctgcttcctg acggccgctc
     9121 agttcgacac tgccgctaac caggccggta gccccttgct ccacgtcgat ggaaagatta
     9181 cgaagaccgg acaccgtctg cccaccgtca caggtcacgg tgaacgcaat tttctggaca
     9241 tcaggtttca gccggttgag cgcgacagta aaggttgagt actggccttc actgaccagg
     9301 ctgacggtac catcatcgtt acgtggctga ccataaaaca ccatgtccgc atcaccctgc
     9361 accttcccgt cggcatacag gcgaaacgcc gaagcatcca cctggccgcc cgaggtgatc
     9421 cggacccgca actcctgggc tggaacgggg gcattgcccc ccggcgtcag gttcattagc
     9481 gaataaccgc tgaaacgata tcggagtgca tatcgtcaat tgtgcggccg cgacaggcat
     9541 ggccaagggc ggtaaagtcc cagttgccgt tattacggcg cagcgaagaa ataacgatcc
     9601 cggtatgtga gccctgctca gtcagcttgt agcgagccag ctctttgccg gtttgatcga
     9661 caacgcggca gaaagcgttt tcgacgtcat tgaacgactg accacggaag ctgtttaccg
     9721 taaacgccag gtattcaacg tttgcaggca ggcgggagag gttgacctta atcacttcat
     9781 cgtcaccgtc accctcaccg gtcaggttat cgccactgtg cacaacagcg ccgcaggtgg
     9841 atttcagttt gcggaaccag atggtgtcaa ttgttttacc tgtgctgtcc attaatacac
     9901 agccagcatc gagatcgatt gagtcgttgc ctccaaacaa tcctccaaga agaccttttt
     9961 tcttaaccgg atcccaaccg agaccgaaat gaagctggct taatgcagat gattctttgc
    10021 tgagtgatac cgtctggttc ttaactaatg aaaccatgtt ttattcctta aaatggtaat
    10081 gaccgagaca atcaaaggta tatttagtaa ctgacgaaga aaatcatatc atgatgaagt
    10141 tctctgacaa gggaaatttt tgtggttttc tgtaaaaaca caaaaagaaa tccttttcag
    10201 ggtgttagtt tttctatttt taatttaaat atcaatgtgt tataaggaat ccaaaaaaca
    10261 ctgacgcact ccataaccac aggagttatt aatcatatta tgattgacat gtagcttaga
    10321 cactcataga ctcacttctc aatcataaat ttgcatggcg ttccggataa ttaaaaaaaa
    10381 tgaacaagaa aatttggttt atggaaggtt tatcctccca gcgagatatt attcaggggg
    10441 taaaatcatt tgcacaaaaa aataattttg ccattaccgt ttttgcctcc caccgtaacg
    10501 aaagaaatga aatcctttcc gttgccgatt attctttgac tgaacctgaa gatcctcaaa
    10561 aacgtcttca gtttatccag gaaaccattc agacctacgg catccaccat attcatactg
    10621 gccgtaacag ccagtggttt gaagaacacc gttcagccat tgaaccgacc ggtgccaccc
    10681 ttactaccgg tgcaacgggc gtcgactggt taactctggc tgacgaaaaa gttacttttg
    10741 ctcagtttat ggagcaaaag ggtctcccgg tcgtaccatc ctggcgggtg aatacgctgg
    10801 cagaattaaa gacacacctc gcggccccgc cgttcactga cagcccggta tgcgtgaagc
    10861 cggtgacggg tatctatggc atgggattct ggcgctttga tgacagtgct tcgcctatgg
    10921 ccgtctttaa tcatcccgaa catcgtctgg tcagtccgca acagtatatt gcagcagcat
    10981 cagctgctga gtcgtttaaa ccccttgttt tgatgccgta cctgccaggc ccggaatttt
    11041 ccgtcgatat cctcgcggat aagggcgaaa tactcgcagc cgtgggacgc cgtaaggaag
    11101 gggctatcca gtatctggta aacgaaggaa gcgcctggga actggcgtgt gactgcgccc
    11161 gtgttatgaa ggccgacggg ctggtgaatg ttcagacgcg aaacgatgtg aatggcaacc
    11221 cggtgctgct tgaaaccaac atgcgtccgt cagggggggt gggttatacc cttcacagcg
    11281 gtgtgaacct tcctgggtta tttgctgcct ttaagctcgg tctgatgtct gaagatatgg
    11341 tacgccagag cgctaaaaac accttttctc cggttgcggt gagatccatt acggatgtaa
    11401 ttgcataccc ggaatcactc tctaaccttc tgaattaagg cgtatttatg tctgatatcc
    11461 tcgaagatat gatttatcgc cgtaccctct catgcggtac gattcaggta acccgtgacc
    11521 agggtgaagt ttcgctcgat gacctgtttg acatcgctga aagacgtaac ccgaaacgcg
    11581 cctttctgtt tgtcagcaaa gtactcggca ggcacattcc cgtgccaccc tcagtcatgc
    11641 ggcaggccta cagacagctt gccagccagt tcccctcgac actgacagga cccgtactgt
    11701 ttatcggtat ggctgaaacc gccgttgggc ttggggccgg tgtatttgat gaagtgcgcc
    11761 accagcatcg tgaatctgtc tatctgacct ctacccgtca cccggttgat ggcacgttgc
    11821 tttgcgagtt caaggaagaa cacagccacg ccaccgatca tctgatctat ctgccagatg
    11881 atgaagagaa aagacgtcgt gttaccaacg cacgaacgct ggttttgatt gatgacgagg
    11941 caaccaccgg taataccttt attaacctgc tttctgccct gcgtaatacc ggcaagcttc
    12001 aacatattga acaggttatc gccgtaacgc tgaccgactg gagtggcaat gccctgtcgg
    12061 agcgcacccc cttgcccgtc acttcggttt cgcttgtaag cggtcagtgg ggatggaccc
    12121 cattacctga tgcacctgtc ccggacatgc cgaaagtcaa cgtaacttca cgaggagaat
    12181 gggacatcca ggggaaacag tcatggggcc gactggggat gctcgcacct gcggccgatc
    12241 tcggccatga ggtctccgtc cacaaggggg aacgtattct ggttctcggg accggggaat
    12301 tcgtctggga gccgttcctg cttgctgaac ggctcgaagc tgccggagca caggcatttt
    12361 atggatcgac cacccgctcc cctgtcgccg ttggttatgc cattgagtcc gccatttcct
    12421 ttacggataa ctacgggctg ggcatcccca attttgtcta taacgtcgcc caccagcagt
    12481 ttgaccgcat tcttgtgtgt actgagacac ccgcagaaag tattgacacg cagcttctta
    12541 aagcgctggc tgaggttgcg cccgtcgtgg agattgttac ctatgaataa acccgttgtt
    12601 ctgagcgacc ttgacgacac gctctttcag acccgtcgta aaatggtgga cgagctggcc
    12661 ctggagccat tccgtaccgg tgccgttgat cgcaccctga atccacgaag ctttatgacg
    12721 gaagaacagt ccatgttggt ggactggctc ctggagcagg ctgaactcat acccgttacc
    12781 gcacggggaa ctgaagaaat cagccgcgta cggatccctt tccactcctg ggcaatcaca
    12841 acgcacgggg ccgtcattct cacgcctgaa ggcaaacccg acgaggagtg gaaggcccat
    12901 atgctcggcc agctggctcc ctaccaggaa aagctgacat cgatgcagcg tctgatcact
    12961 gaaatgatgg acgcaaaagg aatcaatgcc tgggcaaggc tgaacttcga atacggtgaa
    13021 acggcggttt atatggtgat gaaacaccgc gacagcaccc gccttgacga gctcaatgcc
    13081 attgcagatg agatagaaac ggtgtttccg accgagggct tctacatcca ccgcaacagt
    13141 aataacgtgg cctggcttcc caccccggtt gagaaagggc tggcagtcag ctggctgctt
    13201 gaaaaacttc gggctgaacg aggagttttc cccgtaattg gtttgggcga cagcctgagc
    13261 gatcatcgtt ttatgaaact gtgcagctgg tttggcatcc cgcgtcagag tcagtttgca
    13321 gatgccattt cacagcgaat ttttggagaa aattaaaatg cagccacatg aaacatttac
    13381 cggctcatac cagcccggtg acgtggaatt tctgctaaag ccggttgtca ttgagatgac
    13441 gccggttgag aaaaaagaag aactgattca gtccgggaag aaacattatt cggacatgct
    13501 cagccaggag ccagcgccta cacaatggca tcttgatttg tttcaccggg cgctggatcg
    13561 gggcgcagag aggcttgcaa aagaagtcac acagctcgct attgctctgg ccgaacgctt
    13621 cggtgatgag cccattgtac tggccagtct tgtcagggct ggcgttccgc tcggcgttat
    13681 gctgcaccag gccctgcgtg acatggggaa aacctcatgg cattacggta tcagcattat
    13741 ccgggatcgt ggaattgacg gtgcagccct cgacgtcatt gaagagcggc atggtaccag
    13801 cggtattgtc tttgttgacg gatggacagg taaaggcgca attaccggag agcttgtacg
    13861 cgcactgaaa gatcgcccgg gctatcctga gcagccgcga ctggttgtcc tggccgatcc
    13921 ctgtggctgc tcctggcttg cagccagtga tgatgactgg ctcattcctt ttggcatcat
    13981 gggggcgccg gtatcaggcc tgatctcccg gtcagtatgg tcctctgaag gattacacgg
    14041 gtgcatggtt tgcgagcatc tcagtgaatt cgaatgcagc cggatgcttg ccgataccgt
    14101 cgctcatttc cgtaagaagt taacaccgtc atccctcgct cccctgagct ggaatacgga
    14161 atcagcccgg gtcttatggc agacaagtcg cgacgttatc gcgttcctgg ccgatgaatt
    14221 taaagtggac agcgtcaatc gtattaaacc cggtattgct gaagcaaccc gggctgtatt
    14281 acgtcgggta ccggaccatg tatttgtgcg ttctattgac gacccggacg ttgccttgct
    14341 tgtagggctt gctcgtgaaa agggaatagt tgttacagaa atggggggaa ccctcggcca
    14401 gtatcgggct gtaaccatta tcaagaaggt actctgatga aaaatcgtct ttctccctgg
    14461 aatctgggag ccacgctata catgcctgca acacgggaag atattgctga tgccgtcctg
    14521 cacgggaaaa tcccgggctt gcgctctctg gtgatttgcc tggaggatgc tgtcagtgaa
    14581 gcagacatcc ccgtcgccct caaaaatctg gagcacctgc tgcacgagct cagtaacagc
    14641 atgagtagcc tgggtaaaaa tgactggcct ctggtgttca ttcgcccccg gcatgccgaa
    14701 atgggcagat ggttaaaagc gcattatgat ctttccgctg tcgacgggtt tgtgctgcct
    14761 aaatttaccc tcagttcgtt agctgaatgg tgggatatca tggtcgggac tcacctgtgc
    14821 atgatgccaa cgctggaaac agaagatgtc tttgacgtgg ttcagatgcg cgagctggct
    14881 acccgtctgg aggaacatcc ctgccacgac cgcattattg cgcttcgaat cggcggcaac
    14941 gatcttatga acgttgtgtc gcttcgccgt ccccgggacc tgacgctgta tgacagcccg
    15001 atgggctacg tcatcaaaat gctcgtttcg gtcttcggcc cgcgtgattt tgccctgacc
    15061 gcgcctgtat gtgagcatat tgacgatcat gccgttatgg ccagagagct ggctcttgat
    15121 atggcacacg ggcttgttgg gaaaacggcc attcacccgg gtcagataga ggtcattcaa
    15181 aacgcgctga tggtcactca gggtgagcat tctgacgctc tgcggatcct gaactctacc
    15241 caggccgtgt ttaagtcgca gggagcaatg tgtgaacccg ccacacatcg ccgctgggcg
    15301 gctggcattc tggacagggc tcgtttttat gggttacaga acgagcaaag cgctgatgga
    15361 atcagattac ttaccgtgac ccagcatcat taaggaaatc ataatggggt tcaggttcag
    15421 aaagtcgatc aacattattc ctggcgttcg cctcaacctg agtaacggtg caccgagcct
    15481 gagtgtcggg ccgagaggtg cttccgtttc ttttggtagc cgggggacct atgccaatct
    15541 gggcttgccc ggtaccgggc tgagttaccg tacccggctt gaccgggccg cgcgttccag
    15601 aggtgaaaac cggacggcaa ccgacccggg gctcagacag gcgcttgagc agaaagccgc
    15661 tgaactcatg tcagcggtaa ccgcaatccg taatattcac gagctgacgc cggatccaaa
    15721 aacaggcatc agctgggcag agctggaagc agtatacctg cataacagaa cgtcgccttt
    15781 tcaggttccg gcaccggtgc gtccagaaaa gccagactac cttgtattgc cggaaaagcc
    15841 tgccgagagc gaaggcatta gttttttggg taaatggttt gaatcggaat cagctaaagc
    15901 tgagcgccac gccgaaaatc ttcgccggtg gcagcaggag ctgattgatg tggagcgtga
    15961 gaataccctt cgacagcacc ggtaccagca acaacggacg gcctgggccg aacagtatgc
    16021 aaactggaag tttgaagctg aagaacatga aaaacggctc gccacggctc aggcagatgc
    16081 ccggcagcag ttccggacag acgccgcgtt tttcgaatca tacctggcgg gtgtgctggc
    16141 agaaactgaa tggccgcgtg aaacgcttgt tgcatttgaa gtaaagccgg agctatcagc
    16201 agtcctgctg gacgttgatt tagctgagat tgaagatttc cctgataaga tttacggcgt
    16261 taatgcccgg ggaacggagc tgacggaaaa agccatgacg caaaaagccg tacgcgaaaa
    16321 ctatgcccac cacgtccatg gctgcttgtt ccgcctggtc ggtatcgttt tacatacgct
    16381 acctttcgac aacgtgattg tgtcaggctt tacgcaacgg gtcagtaagc ggaccggcta
    16441 tctggaggat gagtatatcc tgtcctgcaa atgcactcgc agccagatgt cgtcagtaaa
    16501 ttttgcaggc ataaaacaca ttgatccggt tgaagcgtta ggcgatcaac cggttattcg
    16561 aaagatgagc agtaccttca tttttcagcc tattgaacca ctaacccttt aagcaggcta
    16621 aatcttacga tgcaattaaa taccagacag gcccggattt ttaagcttgc caacttactg
    16681 ggcaccggca agcccgtttc cgctgccgac attataacca gtctggaatg ctctgagccg
    16741 acattaaccc gggccctcaa agagctacgc gagtcatatt ctgcggagat caaatacagc
    16801 aaggcaggtc attcctatca tctcgttaat ccgggccagt tggataaaaa gaccctccgc
    16861 cggatgaatg aagcgctcgc acagaatgct gaactgaaaa ccggtgagtc cacagggaag
    16921 gtcgtactcg ataaggataa aaaaacagcc gtgtcattat cgctacgcat gcggattcta
    16981 agaaaaattg accgtctggc tgcgctgagt gactcgaccc gaagtgaagc ggtagagaag
    17041 ctggctctgc actccgttga tgaactgata aaagaataca gcgctaaaaa gtcctgacgc
    17101 taaatctcag accggggtga aaactccggt tttgtcatat ttcacctctg tattggcgtg
    17161 atgcaaaatg gcatctgaat cgatcatggt ttggcatcca tttccgatca ctgtttggca
    17221 tcacaatcga tcacgatttg gcatgcttcc gctcattgat tggcatcctg gtgtcgttac
    17281 ggggaagtca tcttcacagg gatcttcagt cagttagcat taccgacatc cattcaccaa
    17341 cttaccggag ataatcaatg tcaaaacccc gatggacgct tgatcagaaa aaacatcacg
    17401 ttgccgcatg gcgcgccagc ggccttaccc gtgaacagta ctgtgaactt tacgacattc
    17461 cgtttaaatc cctgcgccag tggccgcagg atgtcgcaaa agccgaaaaa cgagccagag
    17521 caccagaaat tattcctgtt tcagtatctg gctcttccgg aatgactgat ggcagaccat
    17581 tgtcggatga gcctgtcact ctcttcctgc ccggtggtat ccggatgtgc tgccagcctt
    17641 ctcagttaac cgatgttttc cgggctcttc gccatgctga cgcctgataa cgtattcctg
    17701 gtcgtaaaac ccgtcgatat gcgccggggt atcgacacac tgacacagta cgtccagaac
    17761 gaacttaacg ctgcatggca tgacggtgct gcttttgtct tcaccaataa agtccgctcc
    17821 cgcataaaag ttctccggtg ggacaaacat ggcgtatggc tgtgtacccg acgactgcac
    17881 cggggaagct tccgctggcc ccggaaaggt gatgccacat ggcatctcac acaggatgag
    17941 tttcactggc tggtctttgg ggttgactgg caacaggtga aagggcatga cctggcaaaa
    18001 tgggtatacc agtaagaacc cgaagatgct cagagcaacg caataactca atgaaatata
    18061 atgaatatcc atgcgtgatg tcatataatc caccgcatgg atatctcgct tctcagtacc
    18121 accagtgacc ctgaacagct tcgcgctctg gcaattgcca tggtgcaaaa agtcatggct
    18181 gagaatgcag aacttcagaa ccgtatccgc atcctggaag agcagatgaa gctcgcacgc
    18241 cagcagcgtt tcggtaaaaa atgcgaatcg ctggccggga tgcagcgttc cctgttcgaa
    18301 gaggatgttg acgcggatat cgcagaaatc tcggcgcatc tggacaaact gcttcctcag
    18361 accggggacg aagaaaaaac aacaacacgt cctgttcgta agcccttgcc ttcacctctt
    18421 ccccgtgctg aaaaggttat cccgccagct gaggaacgtt gtcctgactg tgatgccccc
    18481 ctgcacttca ttcgcgatga agtcagcgag aagctggaat atattccggc acaggtcgtg
    18541 gtaaaccgct atatccgccc gcagtacagc tgtccctgct gtgagaaagt gttcagcgga
    18601 aaaatgccgg cacatatcct tccgaaaagt gctgttgagc catcggtcat cgcacaggtt
    18661 gtcatcagca aatataccga ccatctgccc ctgtatcgcc agcagcacat tttctcccga
    18721 atgggggtgg agctccccgt cagtacaatg gcggatatgg tcggtgttgc tggcgctgca
    18781 ctggccccgc tggcaaaatt gttacgccac gagcttctga cacgggatgt tattcatgca
    18841 gatgaaacgt ccctgaggct ccttgatacc cggaaaggcg gtaaatcctg ctccggctgg
    18901 ctgtgggctt acgtcagcgg agaaagagta agcgtcaacg gagcaccgta ttgacgctta
    18961 tttattggtg agtactacgt tccatggcag gagttcatca actcggttgg aaggccattc
    19021 tggcagtacg ctcaggatat ggcgcagata cgcttccgga tcgataccgt tcagacggca
    19081 ggtgccgatc agcccgtaca acagtgctcc acgctcgccg ccgtgatcgc tgccaaagaa
    19141 cataaagttt ttctttccga gacagactgc acgaagcgct ctttccgcag cattattatc
    19201 cgcctccgcc agaccgtcat cactgtaata acagagggca tcccactgat tcagtacata
    19261 gctgcacgct tcgcccagtc tggatttttt cgacagcgta ccattcttct ccaccatcca
    19321 ttcatgcagt gacgtcagta acactttgct tcgctgctgc ctgacggcaa gacgctctga
    19381 ctccggtaat ccccgtattt catcctcgat ggcgtacagt tcactgattc gcttcagggc
    19441 ttcttctgcc gtcgcacttt tgctgctgat gtatacatcg tggatttttc gccgggcatg
    19501 ggcccagcac gcaacttctg tcagtgcacc accttcacgt tctgcactga acagccgatc
    19561 gtaaccgctg aacgcatccg cctgcaggat accccggaag ggacgaaggt gttgtaccgg
    19621 atgttttccc tgcctgtctg gtgagtaggc gaaccagacc gccggtggct ctgatgagcc
    19681 cgcattccgg tcatcccgga catacgtcca gatgcgtcct gtttttgcct tttttctgcc
    19741 cggtgccagc acttttactg gtgtgtcgtc agtgtgaacc ttgcgggtgt tcatcacgta
    19801 acggtacagg gtatcattca gcagagtcat taactgacaa catgcgtcaa cccagttgga
    19861 gagtaatgcc cggctcagtt cgatgccctg gcgggcaaag atttcactct gacgatacag
    19921 tggcaggtgt tcgcagtatt ttcccgttaa cacgcgggca agtaatccgg agcccgcgat
    19981 gccgcgctct atcgggcggg acggcgctgg cgcttcaact atacagtcac attttgtaca
    20041 ggcttttttt acccgaacag tgcggatcac tttcagggcg ctactcacca gttccagctg
    20101 ctcagcactg acttccccca gataatccag ctcactgcca cactccgggc aacaactttc
    20161 ttcaggctcc agacggtgta tttcacgggg aagatgtgcc ggtaacggac gacgatgacg
    20221 tgactgtcgc aactggcggg gaacctgcgg gtcatcctca cgcccactgt aacgatcgct
    20281 ttcctgttcg cgttgtttca gttgggcctc agcctgttca acctcacgct gcagtttttc
    20341 agaacgggta ccgaacaaca tccggcgcag tttttctatc tgggccctca gatgttctat
    20401 ttcccgctcc tcctcttcga tcttttcctc ggcacgtgcc agtgcagagc gcaggaaggc
    20461 ctccgtctct tcaaccagac tcagttgctg atctttctga cggagggctt cagcctgctc
    20521 agagagcagc ctttccagct cagtgatacg aatgaggtat ttccgactca tgaccgtttt
    20581 tataatccgg ccatgacatt tttacaacat tgtcagtgca ttaaggcggg atgttttggg
    20641 ctgacgccag tccagtttat cgaggagcat tgccagctgc gagcgggtaa tggatacctt
    20701 accgtcacgc accgcaggcc agataaactg gccttcctcc agacgtttgg tgaacaggca
    20761 cagaccatca gcatcagccc acagaatttt aatcgtgtca ccccgtcggc cgcgaaagat
    20821 aaacaggtga ccggagaagg ggttctcatc cagcacatgt tgtacctgtt ctcccagacc
    20881 gttgaaggat ttacgcatat cagtaacgcc ggcaaccagc cagattcgag tgcctgatgg
    20941 gagcgagatc atcgtcccct cccggtcagt tcacggatca acaccgtgag cagctctggt
    21001 gaaggatttt ccagcgtcat gttaccgtgg cggaactcaa ctttacagga actggcactg
    21061 actgtgcttt gtgaaggagt ggataaaagc ggagtaagag ccgccatagg ctctttctgc
    21121 tcatcaggcg ttatctcaac aggtaataat tcaacgccag cgccagaaga ggttgttacc
    21181 ggaagacgcc gcgatatacg cctttcgttc tgccagagcc tgagccattt gaacaggagg
    21241 ttatcattga tatcgtgttc ccgggcaata cgggcaacag aggctcctgg ttgtgaagcc
    21301 agtttaacca tttgaagttt aaactcattt gaaaatgatc tgcggggttc tgaggataat
    21361 attttctgtt ccataacagg tgtccactag ttgaaaaggt gggcacctac gttaccaata
    21421 ctggcttaat ggctacatac ggcggtcagt ttacgcttac gagaaagaag cggtccgccg
    21481 gtcgtctgtt ttgactcgca gaccggacgg gccctcagat atcctgaggc ctggctgcag
    21541 ggctggtgtg gcggtacgct ggtgagtgac ggatacagcg tgtacaaatc tctggcggat
    21601 aatgaccccg gtattacatc tgcctgttgc tggagccacg caaggcgcgg tttcgcaaat
    21661 ctgtataagg ccagcaggga gccccgtgca gccatggcgc tgagaaaaat cgcgggtctt
    21721 tatcgcattg agaagttcat ccgtgaacgt cctgtggaaa aaatacggca atggcgacaa
    21781 cggtattccc ggccgatagt gaatgacctg tttgcctggc ctgaagagca ggagccgtgc
    21841 tgtccgcccg acggaccact aaacaaagcg attaattata tcctgaaccg gcgcgatgaa
    21901 ctgagctgct tcctgagtga cggagccgtg cccctggaca acaatatctg cgagcgtgct
    21961 atccgcccgg tggtcatggg gcggaaagca tggttattcg ccggttcact gatggccggg
    22021 aaccgcgcgg cacagataat gagccttctg gaaacggcaa aacgtaatgg tctggagccg
    22081 catgcctggc tgacagacgt tctgacgcgt ctaccggagt ggccggaaga ccgactggaa
    22141 gagttgctgc cccttgaggg atttaccttc tccgggtgag tgatacctgc catcaggtgt
    22201 tcgtgcagcg ggccataacc cgcagtcggg agttgaacgc ctgacgacag gaaatgagcc
    22261 agagcagcga ccacgcgggc tgacagtgag tcagaacctg aatcagcccg gagccattcc
    22321 cggggctgta cttcatcacc tgccatcagt acccgtgtca gtacttcgcg acctgacgca
    22381 attacccgac tcagttcttc agaacccgcc tccgatacca gcctggcggt cgcccctgtg
    22441 caacaaaata aatcaaccat cgtcagacgg tgatcaatcg tatgcgctgc cagtggctcg
    22501 cctgaaaaat gcgtcaatat gagttcgccg gaacaatacc tcagttcttc agaacccgcc
    22561 tccgatacca gcctggcggt cgccccggcg caacagaata aatcaaccat cgtcagactg
    22621 cgatcattcg tatgcatcgc tgatgatgcg cccgaaaaac gcgtcaatat gagttcgccg
    22681 gaatattact gacgacacgg tttgtagtta cgttccttcg gtgagtgagt acctgtgcgt
    22741 cagggtaacg caatcagttc aggtttagcg gggtgtcaag tcggtttggc taaccgaccg
    22801 cagaattaac tgtaccttta aaggtcgccc ccgatatatc tgcctgtcag acatatcgcc
    22861 gattcggtac ctcactacat aaaactcctt aaaaaacaat gcgattctgt tggtacgaag
    22921 ctttcagtaa gtcagtccgg agaagcgcca gtacgataca gaatgaggtt gaaaaaaacg
    22981 ttcacgtctg gtcacttctt caaatgttaa aaaacatctc gtaaagagcc agtttatgcg
    23041 ggttcgttga gtagacgtgc gagcgtcact ttgtcagctt taacgctgac gaaagtgacg
    23101 cccgcacagc ttcaaactcc acatgcacac gcaagcgacc aaatttcaaa tggtcttatg
    23161 ccgtgcgcac agtcataaga agcgcatgcg gaaagtcaga aaatatctta agacgcagat
    23221 tttaaaggcg tttttatgcc agtctgccgg gaatacacag tagtccggtt gtccggaagc
    23281 accacggtaa tccgaaggaa aaatcaacgt acctcaatca ccgtagtgat atccgttccc
    23341 agaggctaaa cggatgtacc accataaagg catcctcttt caggctacga ggatattgca
    23401 atcacccgaa ggcgtttctc tcagattgct gaaacattgg ctggcagtca cccgaaggcg
    23461 tccctctcag actgctggaa cattggctgg tcgcaaaagc gacagttaat gcaaatacag
    23521 gttaacaaaa agggaaatct gccgtcaacc caaccatatg aacatgttga tttcattatg
    23581 aatgtgatgt tctaatataa attaatcagt aacttcataa aaagaatata agaaacagat
    23641 aatgcatgta tttacacaca tatcagcaca atgatagatt accaccccag aaacacgata
    23701 aaggtataat tatggaattt tccgttattt ttttctgggt tatcactggt cttattacca
    23761 tcttattctt aaaatcaata ataaccgatc taataaaaaa cctaaacaaa acagacatag
    23821 cgttatatat attggcattt ttatcagccg gatatacatt ccagtatatt tacaatgaca
    23881 atatattttc agaactatgg ggagttggcc
//
LOCUS       DAZFBR010000077        23372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFBR010000077 DAZFBR010000000
VERSION     DAZFBR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.077"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1232
                     /locus_tag="ACHJZ2_003216"
     CDS             <1..1232
                     /locus_tag="ACHJZ2_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO4569282.1"
                     /translation="KLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSL
                     AFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKD
                     NILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYT
                     KILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRG
                     IEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDF
                     CVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTEL
                     NSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKA
                     LREAKSEGGNKVIIHHI"
     gene            1413..2903
                     /gene="dgcJ"
                     /locus_tag="ACHJZ2_003217"
     CDS             1413..2903
                     /gene="dgcJ"
                     /locus_tag="ACHJZ2_003217"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO4569283.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSVRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2946..3449
                     /gene="yeaK"
                     /locus_tag="ACHJZ2_003218"
     CDS             2946..3449
                     /gene="yeaK"
                     /locus_tag="ACHJZ2_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO4569284.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3450..3554)
                     /gene="yoaI"
                     /locus_tag="ACHJZ2_003219"
     CDS             complement(3450..3554)
                     /gene="yoaI"
                     /locus_tag="ACHJZ2_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO4569285.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3724..4170
                     /gene="yeaL"
                     /locus_tag="ACHJZ2_003220"
     CDS             3724..4170
                     /gene="yeaL"
                     /locus_tag="ACHJZ2_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO4569286.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(4127..4948)
                     /gene="nimR"
                     /locus_tag="ACHJZ2_003221"
     CDS             complement(4127..4948)
                     /gene="nimR"
                     /locus_tag="ACHJZ2_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO4569287.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            5045..6226
                     /gene="nimT"
                     /locus_tag="ACHJZ2_003222"
     CDS             5045..6226
                     /gene="nimT"
                     /locus_tag="ACHJZ2_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO4569288.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLQLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6281..6628
                     /gene="yeaO"
                     /locus_tag="ACHJZ2_003223"
     CDS             6281..6628
                     /gene="yeaO"
                     /locus_tag="ACHJZ2_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO4569289.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6650..6904)
                     /gene="yoaF"
                     /locus_tag="ACHJZ2_003224"
     CDS             complement(6650..6904)
                     /gene="yoaF"
                     /locus_tag="ACHJZ2_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO4569290.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRLDHPAGEK"
     gene            7087..8112
                     /gene="dgcP"
                     /locus_tag="ACHJZ2_003225"
     CDS             7087..8112
                     /gene="dgcP"
                     /locus_tag="ACHJZ2_003225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO4569291.1"
                     /translation="MSDQIIARISQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNQKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8145..8243
                     /gene="yoaK"
                     /locus_tag="ACHJZ2_003226"
     CDS             8145..8243
                     /gene="yoaK"
                     /locus_tag="ACHJZ2_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO4569292.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8246..8320
                     /gene="yoaJ"
                     /locus_tag="ACHJZ2_003227"
     CDS             8246..8320
                     /gene="yoaJ"
                     /locus_tag="ACHJZ2_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO4569293.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8379..8627)
                     /gene="yeaQ"
                     /locus_tag="ACHJZ2_003228"
     CDS             complement(8379..8627)
                     /gene="yeaQ"
                     /locus_tag="ACHJZ2_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO4569294.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8774..8956)
                     /gene="yoaG"
                     /locus_tag="ACHJZ2_003229"
     CDS             complement(8774..8956)
                     /gene="yoaG"
                     /locus_tag="ACHJZ2_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO4569295.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8960..9319)
                     /gene="yeaR"
                     /locus_tag="ACHJZ2_003230"
     CDS             complement(8960..9319)
                     /gene="yeaR"
                     /locus_tag="ACHJZ2_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO4569296.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9492..10130)
                     /gene="leuE"
                     /locus_tag="ACHJZ2_003231"
     CDS             complement(9492..10130)
                     /gene="leuE"
                     /locus_tag="ACHJZ2_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO4569297.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10257..11180)
                     /gene="dmlR"
                     /locus_tag="ACHJZ2_003232"
     CDS             complement(10257..11180)
                     /gene="dmlR"
                     /locus_tag="ACHJZ2_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO4569298.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11283..12368
                     /gene="dmlA"
                     /locus_tag="ACHJZ2_003233"
     CDS             11283..12368
                     /gene="dmlA"
                     /locus_tag="ACHJZ2_003233"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO4569299.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12619..14229
                     /gene="yeaV"
                     /locus_tag="ACHJZ2_003234"
     CDS             12619..14229
                     /gene="yeaV"
                     /locus_tag="ACHJZ2_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO4569300.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14261..15385
                     /gene="yeaW"
                     /locus_tag="ACHJZ2_003235"
     CDS             14261..15385
                     /gene="yeaW"
                     /locus_tag="ACHJZ2_003235"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO4569301.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15441..16406
                     /gene="yeaX"
                     /locus_tag="ACHJZ2_003236"
     CDS             15441..16406
                     /gene="yeaX"
                     /locus_tag="ACHJZ2_003236"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO4569302.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16460..17575)
                     /gene="rnd"
                     /locus_tag="ACHJZ2_003237"
     CDS             complement(16460..17575)
                     /gene="rnd"
                     /locus_tag="ACHJZ2_003237"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO4569303.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17657..19342)
                     /gene="fadD"
                     /locus_tag="ACHJZ2_003238"
     CDS             complement(17657..19342)
                     /gene="fadD"
                     /locus_tag="ACHJZ2_003238"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO4569304.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19547..20128)
                     /gene="yeaY"
                     /locus_tag="ACHJZ2_003239"
     CDS             complement(19547..20128)
                     /gene="yeaY"
                     /locus_tag="ACHJZ2_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO4569305.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(20168..20863)
                     /gene="tsaB"
                     /locus_tag="ACHJZ2_003240"
     CDS             complement(20168..20863)
                     /gene="tsaB"
                     /locus_tag="ACHJZ2_003240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO4569306.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20921..22831)
                     /gene="yoaA"
                     /locus_tag="ACHJZ2_003241"
     CDS             complement(20921..22831)
                     /gene="yoaA"
                     /locus_tag="ACHJZ2_003241"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO4569307.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22963..23307
                     /gene="yoaB"
                     /locus_tag="ACHJZ2_003242"
     CDS             22963..23307
                     /gene="yoaB"
                     /locus_tag="ACHJZ2_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO4569308.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5725 a         5956 c         5675 g         6016 t
ORIGIN      
        1 taaaactcat cccacagatg aattacctga tggttgttgt ggctttgttt ttccttaacg
       61 ccgtcatttt tcttttcatg ttaatgaaat atttcactaa caaacaaatt ttaccaacac
      121 tcattttaag ccttgcattt ttaagtggcc ttatctattt agttgaaacc attgtaatta
      181 tccataaacc aattaacggc agtacactga tccagacaaa gtcgaatgat gtttctattt
      241 tctatatttt ccgccaactc agttttattt gtttaacctc gctggcgctc ttttgttatg
      301 gaaaagacaa catccttgac aacaataaga aaaaaactgg cattctgttg ctggcgctga
      361 tccctttttt agtttttccc cttctggcac acaatctgag cagttataac gctgactatt
      421 ctttgtatgt cgtcgattac tgtccggaca atcatactgc gacctgggga atcaactata
      481 caaaaatatt ggtttgtctg tgggcatttt tactgttctt tattatcatg cgtacacgat
      541 tagccagcga actatggccg ttaatagcat tattatgtct ggcatcgcta tgctgcaact
      601 tacttctact gactctggat gagtataatt ataccatctg gtatatcagt cgcgggattg
      661 aagtctccag taaactgttt gttgtgtctt ttctgattta taacattttt caggagctgc
      721 aactctccag caaactggca gttcatgatg tgctgaccaa tatttataat cggcgctact
      781 ttttcaacag cgtagagtca ttattgtcgc gacctgttgt taaggacttc tgtgtcatgc
      841 tggttgatat taatcagttc aaacgcatca atgcccaatg gggacatcgt gtgggtgata
      901 aagtgctggt ttcaattgtc gatattatcc agcaaagcat ccgccccgat gatattttag
      961 cgcgactgga gggtgaggtg tttggcttgc tatttaccga actcaatagt gcccaggcaa
     1021 aaatcattgc ggaacgtatg cgtaaaaatg tcgaactcct gaccggcttt agtaacagat
     1081 atgatgttcc tgaacaaatg accatcagta ttggcacagt tttttcaacg ggtgacacgc
     1141 gtaatatctc gcttgtcatg acggaagcag ataaagcctt acgcgaagcg aaaagcgagg
     1201 ggggcaacaa agtgattatt catcatattt aagtgcaaaa atattcagag ccatgctttt
     1261 tgcgtggctt ttgcatacaa tttattacta ttacccctaa atttcccctc cccagcggcg
     1321 cagagatgag tataattagc gcccctgtgc caggccgcaa tcgaacttta tctggttttc
     1381 tcgtttcact aaccgaagga gtgccattta tcatgaaatt gcaccataga atgctccggc
     1441 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
     1501 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
     1561 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
     1621 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
     1681 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacat
     1741 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
     1801 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
     1861 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
     1921 cggttaatgt tgaatacttt gcgatgaata actggtccat cctgcagtca ggaagtattg
     1981 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
     2041 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggtg tatgttgcag
     2101 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatctgcgg aatatctttt
     2161 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gatacagatt
     2221 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
     2281 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
     2341 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
     2401 tgcggatgca cttccgctta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     2461 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     2521 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagctaataa
     2581 atgacaccct cggtcatcag gaaggggatt tagcgattac gttgttagct caggcgatta
     2641 aacaatcggt tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     2701 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     2761 tgcaacatat cgcgccgcag aaagagatcg gcttctcttc cggtatttac gcgatgaaag
     2821 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     2881 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     2941 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     3001 cacaagaagg tgctgatttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     3061 tgtcagaaat tcgtggcacc gctctaggcc agggtgctaa ggcactggta tgtaaagtca
     3121 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc agccgatcaa caggccgatc
     3181 tgagccaact cgccagccat ataggcggat tacgcgcttc gttggccagc ccggcggaag
     3241 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     3301 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     3361 ggatgctgga taaatccgtt attttgaaaa ccgcggatta tttgcgcatt gcgcaaccag
     3421 aactggtcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     3481 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     3541 atttgatcgt tcatagcata cccccgggag atagctgttt tcacgttatt gttagcgtgc
     3601 acaaatggca gtttgatgac agttcgcctg attttttatg caaaaaacgt aaagattttc
     3661 tacttccttc ctgcagcaag cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     3721 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     3781 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     3841 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     3901 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     3961 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg ttattgtctc ctggctgggt
     4021 gggcgcggcg tgacgttaat gggcagccag ccgcaactgg ttgctgggct gctggtgggc
     4081 actgttttag gcgtagcgct gtttcgcggc gtaccggttg gcccgcttat tgccacgggt
     4141 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     4201 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     4261 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagcgccata atcagctgta
     4321 actgctgtcg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgggcaagat
     4381 tgcgttcact catcgcaaaa aagccagccc attgccccaa tgccccccac tcgacgggct
     4441 ctttcgccat catctccacc atcgcgcgga ttttaggatg agaagaaacg ggtaggtgca
     4501 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     4561 tcggctcagc acgctgagtc gtcgtcctgt ttgccagagt taaaattaat tcccggcata
     4621 agggcgatat tttcaatgtg taacatgttg tcggcattgt cactgcgctg ggttcgataa
     4681 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     4741 tccaaaccgc atattgtggc ggcaccatcc acaaagcatt ttccaccgta caggtaattg
     4801 caccatgtag cgccagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     4861 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     4921 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     4981 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     5041 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     5101 tctgatgatt gccactacat tacgcgtcac cttcaccggc gcagcaccgt tactcgatac
     5161 gattcgttcc gcttactcgc tgacgactgc gcaaaccggc ttattgacca ccctgcaatt
     5221 attggccttt gcgctaatct cacctctggc tgccccggtg gctcgacgtt ttggtatgga
     5281 acgtagcctg tttgccgcgt tacttttgat ctgtgcaggt atcgcaattc gctctctccc
     5341 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggtaa
     5401 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     5461 cgcatattcc ctgacaatgg gagctgcggc ggctctggga tcggctatgg tagtgccgct
     5521 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     5581 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacttc
     5641 acgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     5701 tcttgggatc aactcactgg tctattacgt gattattggc tggcttccgg cgatcctcat
     5761 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     5821 agcagctccc ggtttgctga tcccactttt cttacatcat gtgaaagacc agcgtggtat
     5881 tgcagcgttc gttgccttga tgtgcgcagt gggcgcggtt gggctctgct tcatgccagc
     5941 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     6001 gttgacgttc attggtctgc gggctagttc tgcgcatcag gcggcggcac tctcggggat
     6061 ggcacaatcc gtcgggtatt tgttggcagc ctgtgggccg ccgctgatgg gtaaaataca
     6121 cgatgctaac ggtgactggt ccgtaccact aatgggtgtt gcaatacttt cactactgat
     6181 ggcgattttc ggactttgtg ccgggagaga caaagaaatt cgctaatatc cggtactata
     6241 gtgacgtgac aaatcatgcg tgaaagggag aacaaacacg atgaatattc agtgcaaacg
     6301 cgtttatgat ccggcagaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     6361 acgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     6421 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     6481 atatcttgca gaactggcac aacacgagca agaaggaaag cggctggcgg acatcgccaa
     6541 aaaacagccg ctgaccctgc tctactcagc aaaaaacacc acgcagaacc atgcgctggt
     6601 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     6661 cagccggatg gtccagtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     6721 tgcaattgtt gctgacccct tgcgctgtct gcactggaat gagcgtcccg cccttctgct
     6781 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt gcttctggct
     6841 gagaaggggt tgaacaacca gccaggacca gcaagcaagg cagaacaaaa ctgataattt
     6901 tcatttattg atctcacata tgtatccaag cttagagtat cgcggtatcg ttttgttttg
     6961 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttgat
     7021 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     7081 tgtttcgtgt cagatcagat tatcgcccgc atctcgcaat cccttgccaa agaacagtca
     7141 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     7201 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     7261 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     7321 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     7381 attgcggcac gcaatcttgg catcaccaca tttctgagca cgccaattca cttaccggat
     7441 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     7501 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     7561 ctggttgaac agctgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     7621 ttaaccgggc taccgaatcg gcgggcgatt tttgaaaatc tgacgacgct gttttccctc
     7681 gcccggcatc ttaaccaaaa gataatgatc gcgtttatcg atctggataa cttcaaatta
     7741 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     7801 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     7861 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     7921 caaatacgcg gagaatatca cttaggtgat gttgatttgt attatcctgg tgccagtctt
     7981 ggcatagtag aagtcgatcc tgaaacgacc gatgcagaca gtgccctaca tgctgccgat
     8041 atcgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     8101 ccacattcct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     8161 tttttccggt tgtaatcttc attacagcgg tcgtattttt agcatggttt tttattggcg
     8221 gctatgctgc cccgggagca taaagatgaa aaaaacaacg atcattatga tgggtgtggc
     8281 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacatct aaaaaatagc
     8341 aaaggctgcc tgtgtgcagc ctttgtgcaa tttacgcgtt aacttttaat cttcctgtag
     8401 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     8461 tcgactttac caaagccaaa cagcgtgctg atccaaccgc cgactacggc accgactatc
     8521 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     8581 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     8641 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     8701 aagatttgtg acgagcatca cggaggtttt tttgcgatgg cgcagaaatt gcgccatcaa
     8761 cgatcagtga taattaccaa ccacaaacat catgttcgtt ttccgtgtca taagaacgca
     8821 cggtattcac cagatctttt atcacttcag ccgccacttc tggcaccagc aaagtcatcg
     8881 gcgtctctgt ttcataatcg acagaaacgc cattgctgtt attggtgacg gtcacggtat
     8941 acgttgcttt gcccatgatt catttcccgt tatgaatgac tttccgttgt tgcgcacctt
     9001 ccatcaggac ttcaggagcc acgaagaagt caatgttgaa ataagtatcg tcagtcatgg
     9061 cttcaatgtt gtgccacttt tctggaggga acaccgcaaa ctgccccgct tcgataagga
     9121 tcacctgatc aggctctgca ctgtgttcat cagcgtagcc gagatatttg accgccccat
     9181 gcataacgga aaggcgtggg taaacccccg ggcgcgttcc tttatcaaga tgacgttcga
     9241 atattccggc aggcgcagtt tgtttattcc agaaaggcgt tgagcgcgta tgaatataat
     9301 tctgtgggat ttgcagcatc cttttccctc cttcggtgaa tgcgctgaaa acggtttatt
     9361 ccagccgttt cagggtacgc ctgataattt gctttttaaa taccatttat tggttacttt
     9421 ttagcaccat atcagagaaa aatcagggag gattatagat gggaagcccg cgacaacgcg
     9481 ggctgaaagc atcaggattg cagcgtcgcc agtcgggcag cgaaacccac gaacatcaaa
     9541 ccaatcagtg agttgcccac tttagccagt ttctttttgg tacgtatgta ctgcgtgaca
     9601 aaagcaccag atataatcag gaagctcaaa tagcagaaac tcaccagttc cagcgtcgtc
     9661 gccagaataa agaatgaaat tcccgtatgt ggggcattaa catcgataaa ctgtacgaaa
     9721 aacgacacat agaacaaaat ggctttcgga ttagtcaggc tcaaaattaa cgcgcgttta
     9781 aaaatagcac cgtattgggg ctcatcggat ttggcctcgt tatttttacc cttcagggtc
     9841 gcgtaaagaa ttttactccc cagatagagc aaataaaacg caccaagata acgtacaatg
     9901 ttgaataata tcggggtggt cttaattaat gtcgccattc cagcccatgc cagaaacatc
     9961 aataccgcat cgccaataaa tacaccgcag gccgcaagat aaccgccttt cataccgcta
    10021 ctgacgctat ttttgagtac aaacagggta tttggccctg gcaccaacac aataaaaatg
    10081 gccccaacca gataggtcca gtaattcaga accccgtatt cagcgaacac attaacctct
    10141 ttaattatct ttcgatcatg cgcgattaaa ggtgaatatg ctaaccaatc tgtagcggct
    10201 tagaaaggag aaaatcaggt tttaacctga tatcaacccg ataattgaat cattaatcag
    10261 gcatgctcca gtgaaaaatt cgggtagtgc tcggcaaaat actggcgtaa aaattctacc
    10321 gttatccgca ctttcgctga cgtcgccagc cttgaaacat aaacggccca gacgttcgct
    10381 ggctggtaat attccggtag cacttgcact aaatgaccac tggcaatgtt ttcgctaaca
    10441 tcccaccagg aacgcagagc aatcccctgc ccatcgagac accactgatg gacaatttct
    10501 ccatgattag acgacagtgg gccggtgact ttaatcgcat ggtggccttc tttattgcgt
    10561 aattgccaga caccaaaagg atgatcgcgc tctttaatga ccaggcacgg caatgcagaa
    10621 agatcggtta aatgtttcgg cgcgccatgt tgcgcaataa attccggcga cgcgcagaga
    10681 atgcggtaat tggtcgccag tttgcgggcg attaaattag gggcgatgtc atcgccaatg
    10741 cgaatatcga gatcgacacc ttcattgacc aaatcgacca gccggtcttc cacatcaaaa
    10801 cgtaattcca gttgcggata cgccttcgcc agcgctgata acgccggagc caccacctgt
    10861 cgtccaaaac caaagctgct gataatacgc agcatcccct gtggcacctg acgcacgtca
    10921 gaaagttcgt ccatcatctg accgacatcc tgcaaaatcc gctgcgccca ttcataaatc
    10981 cgctctcctt cttcggtaat ggtgacgcgg cgggtggtgc ggtgtaacaa caccacgttt
    11041 agcgtttgct ccagcaaggc gatgcgcttg ctgacgaacg ccggtgaaac gcccagttct
    11101 tccgccacgg cggcaaaacc agcccggcga gccaccagca taaagacgcg caaatcattc
    11161 agcagcggta aattattcat gattcgtgtt ttatgtttca ccagttacgg ggattaattc
    11221 cttttcagtc aattatagga tggtgatgtt gtcaattttg atggtcagga agtgagaacc
    11281 caatgatgaa aacgatgcgt attgctgcga tcccgggaga cgggattggc aaagaagtcc
    11341 ttcctgaagg gattcgcgtg ttacaggctg ccgctgagcg ctggggcttc gccttgagtt
    11401 ttgagcaaat ggagtgggcg agctgcgagt attacagcca tcacggtaaa atgatgccgg
    11461 acgactggca tgagcaactt agccgtttcg acgccatcta ttttggtgcc gtcggctggc
    11521 cggacaccgt tccggaccat atttcgttgt ggggttcgct gctgaaattt cgtcgtgaat
    11581 tcgaccagta cgtcaacctg cgcccggttc gtctctttcc tggcgttccc tgcccgctgg
    11641 cgggaaaaca gcctggcgac atcgattttt acgtggtcag ggaaaacacc gaaggcgaat
    11701 attcctcgct cggcggtaga gtgaatgaag gtacagagca tgaagtcgtc attcaggaat
    11761 cggtatttac gcgtcgtggt gtcgatcgca ttttgcgtta tgccttcgaa cttgcgcaaa
    11821 gccgcccacg taagacgcta acttctgcca ctaaatcaaa cggtttagcc atcagcatgc
    11881 cgtactggga tgagcgagtg gaagcaatgg ccgagaatta cccggagatc cgctgggaca
    11941 agcagcatat tgatattctc tgcgcgcgtt ttgtgatgca gccggaacgc ttcgatgtgg
    12001 tggtggcgtc caatttgttt ggcgatatcc tttccgatct tggcccggcc tgcaccggca
    12061 ccattggcat tgccccatcc gccaacctga atccggaacg cactttcccc tcgctcttcg
    12121 agcctgtcca cggttccgcg ccggatatct acgggaaaaa tattgctaac cctatcgcca
    12181 caatttgggc cggggcaatg atgctcgatt ttctcggcaa tggcgatgag cgtttccagc
    12241 aagcgcataa cggtattctg gcagcgattg aagaagtgat tgctcacggg ccgaaaacac
    12301 cggatatgaa aggcagtgcc accacgccac aggttgccga cgcgatttgc aaaattattt
    12361 tgcgttaagg tcaaaccagt ttatttgaac cgcgtcactg acgcggtttt gttattcgtt
    12421 ctttgcagtt aataacctgc gtcatttcac cttttattgt ttccgtttcg tgttttatgg
    12481 ctttccgtat tcttaattgt ttaatttatg taacatgcaa ataatcaatt cgttaaacac
    12541 gacatacaac tcattaccct gcaaataatt acaactggca aaagccagag acgggctttt
    12601 atgcccagga gatgactcat gatgagcaat gttaagaaaa aagatgtgcc gctgataagc
    12661 atcagcctgg tggccattct tttcatcgca gctgcattaa gccttttccc acaacaatcg
    12721 gccgacgcgg ccaacgcaat atacactttt gttacgcgta cgttaggttc cgcagtacag
    12781 gtattggttt tgctggcaat gggactagtg atttatttag ccaccagtaa atacggcaat
    12841 attcgtcttg gcgaaggaaa accggaatac agcacgctct cctggctgtt tatgtttatt
    12901 tgtgccggtt taggttcttc tacgctttat tggggggttg ctgaatgggc ctattattat
    12961 caaacgcctg gattaaatat cgcaccgcgt tcacaacagg cactcgaatt tagcgtccct
    13021 tactccttct tccactgggg catcagcgcc tgggcaactt atacgctggc ctcattaatc
    13081 atggcttatc actttcatgt gcggaaaaac aaaggtctga gtctttccgg cattattgcc
    13141 gcgattaccg gcgttcgccc gcaaggccca tggggaaaac tggttgattt gatgttcctg
    13201 atcgccactg tcggcgcact gactatttcc cttgttgtca ccgcagcaac ctttactcgt
    13261 ggactttccg cactgaccgg tttacccgat aacttcaccg tgcaggcatt tgtgatcctg
    13321 ctttccggcg gcattttttg cctaagctca tggattggta tcaacaacgg tttgcaacgt
    13381 ctgagcaaaa tggttggctg gggcgcgttc ctgctgccat tactggtgct gattgtcggt
    13441 ccaaccgaat ttattaccaa cagcatcatc aatgccatcg gcctgaccac gcaaaacttc
    13501 ctgcaaatga gcttattcac cgatccgctt ggcgatggtt catttacccg caactggacc
    13561 gttttctact ggctgtggtg gatctcatac acccctggcg tagcaatgtt tgtcacccgc
    13621 gtttcccgcg gtcgtaagat taaagaagtt atctggggac tgatcctcgg cagcaccgtc
    13681 ggttgctggt tcttcttcgg cgtaatggaa agctatgcca ttcatcagtt tatcaatggc
    13741 gtaatcaacg tcccacaggt gctggaaact ctgggcggcg aaacagctgt acagcaagtt
    13801 ctgatgtcgt tgccagccgg taaattgttc ctcgccgcat acctgggcgt gatgattatt
    13861 ttccttgcct cgcatatgga tgcagtggcc tacaccatgg cggcgaccag tacgcgtaat
    13921 ctccaggaag gtgacgatcc tgaccgtggg ctgcgtcttt tctggtgcgt ggtgatcact
    13981 ctgatcccgc tttccatctt gtttaccggt gcttcgctgg aaacgatgaa aaccaccgtc
    14041 gtgctcacag cccttccctt cctcgtcatt ttactggtga aagtcggcgg atttattcgc
    14101 tggctgaaac aggattacgc cgacattccg gctcatcaag ttgaacatta tctcccgcag
    14161 acaccggttg aagccctgga aaaaacacca gtgctccctg cgggaaccgt attcaaaggc
    14221 gacaactgag cgccatcatc ctaacgataa aggtatccct atgagcaatc tgagccctga
    14281 ctttgtacta cccgaaaatt tttgcgctaa cccgcaagag gcgtggacca ttcctgcccg
    14341 tttttatacc gatcagaacg cctttgaaca cgaaaaagag aacgtcttcg ccaaaagctg
    14401 gatttgcgtc gctcacagca gcgaactggc gaatgccaat gattatgtga cgcgtgagat
    14461 cattggcgaa agcatcgtgc tggtacgcgg tcgtgataag gttttgcgcg cgttctataa
    14521 cgtgtgtccg caccgtggtc atcagttgtt gagcggtgaa ggaaaagcaa aaaatgtgat
    14581 tacctgccca tatcacgcat gggcattcaa actcgatggc aatctggccc atgcacgtaa
    14641 ctgcgaaaac gtcgccaatt tcgatagcga caaagcgcaa ctggttccgg tgcgtctgga
    14701 agaatatgcc ggattcgtct tcatcaacat ggaccccaac gccaccagcg tagaagatca
    14761 gttacccggt ctgggcgcga aagtgctgga agcctgcccg gaagtccacg atctgaaact
    14821 ggcggcccgc tttaccaccc gcacgcctgc caactggaag aacattgtcg ataactatct
    14881 cgagtgctat cactgtggtc cggcgcatcc aggtttctcc gactccgtac aggttgatcg
    14941 ttactggcac accatgcacg gtaactggac gctgcaatac ggtttcgcca aaccgtccga
    15001 acagtcgttt aaatttgaag agggtacgga tgcggcattc cacggtttct ggctgtggcc
    15061 gtgcacgatg ctgaatgtca ccccgatcaa agggatgatg acggtcattt atgaattccc
    15121 ggtggattct gaaactaccc tgcaaaacta cgatatttac ttcaccaatg aagagttaac
    15181 cgacgagcaa aaatcgctga ttgagtggta tcgcgatgtg ttccgtccgg aagatttacg
    15241 tctggttgaa agcgtacaga aagggctgaa atcgcgtggc tatcgtggtc aggggcgcat
    15301 catggccgac agtagcggta gcggcatttc cgaacatggt atcgcccatt tccataatct
    15361 gctggcgcag gtatttaagg actaatgaca tcggcggcgg tattttccgc cgccgggctg
    15421 atttttgatg gagtacagca atgtcagact atcaaatgtt tgaagtacag gtgagtcagg
    15481 tggaaccgct taccgaacag gtgaaacgct tcacgccggt ggcaaccgat ggcaaaccgc
    15541 tgcctgcgtt caccggagga agtcacatca tagtgcagat gagcgatggt gataaccagt
    15601 acagtaatgc gtattcacta ctgagttcac cgcataacac ctctagttat cagattgccg
    15661 ttcgactgga agaaaactcg cgcggcggtt cccgcttttt gcatcagcag gtaaaagtgg
    15721 gcgatcggtt aacgatctca acgcctaata acctgtttgc gctaattccc tcagccagaa
    15781 agcatctgtt tatcgcgggc ggtattggta tcaccccttt cctgtcgcac atggcagagt
    15841 tgcaacacag cgacgtcgac tggcagctac attactgctc gcgaaatcca gaaagttgcg
    15901 catttcgtga tgagctagtc cagcatccgc aggctgagaa agtccatttg catcattcat
    15961 caaccggaac acgactggaa ttagcgcgat tattggcgga tatcgaacct ggcacacacg
    16021 tttatacctg tggccccgag gcgctaattg aagcggtaag aagtgaagct gcgcgtctgg
    16081 acatcgctgc cgatacgctg cactttgagc aatttgctat cgaagacaaa accggcgatg
    16141 catttaccct ggtgcttgcc cgttccggaa aagagtttgt ggtgccggaa gagatgacta
    16201 ttttgcaggt tattgaaaat aataaagccg cgaaagtgga atgtttatgt cgtgaagggg
    16261 tatgcggaac ctgcgaaacg gcaatactgg aaggtgaagc tgaccatcgg gatcagtatt
    16321 ttagcgatga agagcgtgcc agccagcaaa gtatgttgat ctgttgttcg cgtgcgaagg
    16381 gtaaacgcct ggtgttggat ttgtagtttg ccgaagccgg atgtggcgct gagcgcgccc
    16441 agtccggctt cggaaaattt tactgcggat attcctgcaa taaattgtgt aatgcttccg
    16501 ccatcagctc accacgccag ccggaaatca gctccggcaa attgttctgc ggtttcagtt
    16561 tccagtgcca gttcagcagt tggttgattt gccgacgcga tgccagcaat tcggcgctga
    16621 tcttgtgcgt ttcgctcacg tcagtaatca gcgacttaat cgctttaaac gctttacgat
    16681 aacccggcat gtccatcagg ttaagcatcg gctgcggtaa ggcctcttcc ggcaatgtct
    16741 gcgctttttc caccagcgcc agcagcgttt taccgtgaaa gcggatttcg ctaccggata
    16801 aacccaggct gtccagttcg cctaaacttc ccggcatata acgcgctacc gaccacaaat
    16861 gctcttcacg cacgacaaag ttcaccgcca gatcgcgctc tcgcgccttg cgcagtcgcc
    16921 agtcggctaa cagttgcaga caggccagtt ggcgtgtgcg taattgccag gcattggtga
    16981 tatcacgcca ggcatcttcc ggcgcaacga cttcctgacg acgcatttgc atcaggcgac
    17041 attcatccag cgccgctggt agccagccgg aggcctccgt ttctaccata agcttggcgg
    17101 tgatcggtaa cagataccag acatccgccg ctgcatattc acactgacgt tcggtcagcg
    17161 gtctggccag ccagtcggtg cgcgattcac tcttgtccag cgtaacgccg gaatactctt
    17221 ccaccatgga agcgaaaccc catgacatcg ggcgtccgca gaaggcagca aggatttgcg
    17281 tgtcaatcag gggttgtggt aattcgccaa agacattgag gaacacttcc agatcttcac
    17341 tgcctgcatg gagaaatttt gtgatggacg gatcgcgcag gatcgctttc agcggtgacc
    17401 agtcggtgat cccgagagga tcaattagcg ccagatgctc gccatcgaaa agttgaatca
    17461 accccagctg cgggtaataa gtgcgcgtac gaacaaattc agtatccagg gctatcgccg
    17521 gaaaggcacg gacggcttca cacaaagaag ccagcgcatc gtccgtggta atcatttggt
    17581 aattcaaatt gttttctctt tagtgggcgt caaaaaaaac gccggattaa ccggcgtctg
    17641 acgactgact taacgctcag gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt
    17701 ctcgtcgcaa aatttttccg acgttagatt tcggtaactc atcacgaaac tccaccagct
    17761 tcggtacttt gtagcccgtg agctgacggc ggcaaaaggt caccagtgac tcttcggtaa
    17821 gcgatggatc ttttttcact acgaagattt tcaccgcttc accactggag ccggaaggta
    17881 caccaacagc cgcgacttcc tgtacgccag gatgctgcat gacgacatct tcaatctcgt
    17941 tgggatagac gttaaaaccg gaaaccaaaa tcatgtcttt tttacgatcg acaatgcgca
    18001 ggaatccttc ttcatccatt accgcgatgt cgccagtgtg taaccagccg tttttgatga
    18061 tttcatcggt agcatccgga cgctgccaat agcccagcat cacctgtgga cctctgacgc
    18121 aaagttcacc tggctgacct ggcgatactt cattatcgtc gtcatccacc agtttggctt
    18181 ccgtcgacgg caccggcaaa ccaatgctac cgctatgata atcaatatca taggggttca
    18241 cgctgaccag cggcgcacat tcggtcaggc cataaccttc cagcagatac tgtccggtca
    18301 gtttcaccca gcgttctgcc accacttgct gaacaggcat cccaccgcct gcagaaaggt
    18361 gcagactgga gaaatccagt tgttgaaact ctttattgtt cagcaacgcg ttgaacaagg
    18421 tgtttacgcc ggtgatagcg gtaaacggat atttcgccag ctctttgacc agccccggaa
    18481 tatcccgcgg gttagtgata agcaggttct gcccacccag ttcgataaac agcaggcagt
    18541 taatggtcag tgcaaaaatg tgatacagcg gcagcgccgt caccaccagc tctttgcccg
    18601 gatgcagcag aggcccatac gtagcgttaa cttgttccag gttcgccagc atattgcggt
    18661 gagtcagcat cgcgcctttc gccacacctg tggtgccgcc agtgtattgc aggaaggcta
    18721 aatcttctgg caccagttca ggtttgacat actgcatacg gtagccgttg tgcagggcgc
    18781 tacgaaatga aatggcatct ggcagatgat atttcggcac cagacgtttg atgtatttaa
    18841 caacgaagtt aaccagtgtg cctttcgctg tggaaagctg atcgcccata cgggtcagga
    18901 taacgtgctg aactgcggtt ttatcgacca ctttttccag cgtgtgagca aagttggaca
    18961 caatgacaat cgccgatgcg ccgctatcgt taagctgatg ctcaagctca cgcggggtat
    19021 acaacgggtt aacgtttacg acgatcatcc cggcacgcaa aatgccaaac agcgccaccg
    19081 gatattgcaa taaattaggc atcatcaacg caacgcgatc gcctttcttc agccccaacc
    19141 cttgttgcaa ataagcggca aacgcgcgac tgcgttcttc cagcttgcgg aaggtcatca
    19201 cctcccccat attcacaaac gcaggctgat cggcgtagcg cgcgaccgac tgctcaaaca
    19261 tatctaccag agattgataa cggtcagggt tgatctccgt cggaacgtcc gcgggataac
    19321 ggttaagcca aaccttcttc aattcttcac ctctaaaatg cgtgttcgtc gtcatcgcaa
    19381 ccccaaatga tatacatgcc gttaacataa tattaactca tcataccagc ttgataatta
    19441 cccaacgaaa aggttgcgaa gcgcgtcact atttattttt atctttaccg taagaatgca
    19501 gaaacagcgg accagccgct gtttcttttt ctttaaaaac aagcaattat tcagttacaa
    19561 ctgtttgtac tctcgcgggg ccgggattat accagcccca tccgccgtgg ccatagggcc
    19621 agccacgacc gccataaaac cagggatcaa tcggctgagg cggcataatc acctgctggg
    19681 ttaaatgcca acgtttgtaa cctgttgcct gcatcaccat aaatttatag ggcgtgttgc
    19741 cgatttttcc gtcaaccgca ccagtgattg gcccgactac cgtaaccagt tgtccacgga
    19801 aatccaccgg gtccagaaaa ccgttcacat cggcataaat gcgaccgcga gaaggttctc
    19861 ccagcgtcgg tctggctccg ctgtccagcg gtacggtagc aatttccagg cgggttttcc
    19921 cttgctggtt ttgtaccgca accactttgc caccaaagcg tgcctcctga ccaacgtaca
    19981 gctgcggcgc actcatcacc cgaactaaat cttgttgcgg cgtgggactg ctgcctttaa
    20041 tggcgtccgg cacagtgaca caaccgctca gcattagcgc aaacgtacct gccagtatgc
    20101 ctttgataac attcttttga accgccatga tgcgactcct ttttttctca gggcatactc
    20161 ttaagattca ttctttgccc ggaagtttct tccatgcgac gttgttacgt aaataaaccg
    20221 gttcggcatg ttccaccgcc accgttttac cctcggcaaa catctgacaa gcaatcggca
    20281 gcatatcttc tgcagcgggc agtaaaactt ctccgtcgcg caaaaccagt ccgctctctt
    20341 taccgagatc cggccaggct tgccagcctg tacctaccgt cacccattcc ccggaaagct
    20401 gttgcattcg ttcatgaacg agttcgggtt tgagtacggc ttcggtttct tcaccgtgcc
    20461 agataccgtt ttcatcacgc tgatattcgg cccagtaaac ttcgcccatt cgcgcgtcaa
    20521 tggctgacag cacgcgtgtt gcgccgtttt tgcgccacgc cccttgcgcc atcgtcatta
    20581 gcgtggagac gccaatcatc ggtaattccg cgccaagcgc cagcccttgc gcgatgccaa
    20641 taccaatgcg cacgcctgta aagctaccgg ggccgcgccc gtaagccaga gcgttaatat
    20701 cagtcaggga agttccgctg gtggtcagga tatcctgcac catcggtaag attcgttgag
    20761 tatgttcacg agggcaaagc tcaaaatgag cgttgacagt accgtcgttc cacagggcga
    20821 cagagcaggc ctctgtcgcg gtatcgatag ccagaattcg catgggtctt cgtgcttaga
    20881 tcaataaaaa ggcgcgcatc ataccatact ccgtaacaaa ttacctggag gatggtatcg
    20941 caaggaaacg aaccgcacgg gcaatgtcac gggtgcgtgg cgcgggcggc agactggcga
    21001 gaaacgtcgc gccgtaagga cgcatcacca gccgattgtc acaaattacc aaaaccccgc
    21061 gatcgtcggc gtcgcgaatc agtcgcccga ctccctgctt gagagtaatc accgcatccg
    21121 gcagttgtac ttcatcgaac gggtcgccac cgcgcaaacg gcaatcttcc atgcgcgctt
    21181 ttaacaatgg atcatcaggc gaggtaaacg gcaatttgtc gataatcacc aatgacaatg
    21241 tatcgccacg cacgtccacc ccctcccaga aactactggt tgccacaaga agcgcgttac
    21301 cggcactgac aaattgctgc aacagttgcc ctttgctggt ttccccctgc aataatacgg
    21361 gaagcgtcat ggtagcgcgg aactgctcgg ccagatcgcg catcatggcg tgcgaggtac
    21421 aaagcataaa acagcgaccg ttgttagctt cgatgatcgg gcgcagcata gccgccagtt
    21481 gccgcgcgga acccggttga ttggtttgcg gcaggttgcg cggcacacag agtaacgcct
    21541 ggcggctgta atcaaacggg cttggcaata gcaacgactc cgcctgttct atcccaagcc
    21601 gcgaggtgaa atgatgcaga tcgtcgttta ccgacagcgt tgctgaggtg aagatccagc
    21661 taccgggctt ttgcgccatt aactctttga atttatccgc cacgctgagc ggcgtgagag
    21721 ccagagtaaa atggcgcgaa gtgcattcgt accagtagct gtagcccggc tgattgatct
    21781 cttttagccg cttcagccgt gtgcgataca acgtggcgcg ctcaaatgcc gcatccagca
    21841 aggcggaacg ccccagtgac agtttcgcca cgtcataaca aagttccagg gtgtcatcga
    21901 gcagtaaaaa tgcccgctga atttgcggat tagctaacag ctcacgcagg ttaccgcgat
    21961 aacctggttc accgagttgc agacgaaaat cctgcgcact ctgggcaaga cgatcagcgc
    22021 acttttgtaa ctgctgggtg tcttttaatt cggtgcggta ggcgatggtg atgtcttttg
    22081 ccaggtcgag cagttgtcga ctggagagtg actgaccaaa atactggctg gcaatgtccg
    22141 gtagctggtg ggcttcgtcg aagatcatga cgtccgcttc cgggatcaac tcgccaaatc
    22201 cactctcttt aaccaccata tccgccagaa agagatgatg gtttaccacc accacatcgg
    22261 cgtccatcgc ttttttacgt gctttgacca caaagcaatc tttatacatc gggcagtcgc
    22321 tgccaagaca gttgtcgttg gtgctggtga ccagcggcca cgcctgcgaa tcttccgcca
    22381 cgctgacgca ggtgctgata tcaccatcga ctgtttgatt agaccaggag cgcagcagga
    22441 tcacatcgct taatatttgt accggaagat cgccccccgc cagcgcctgc tgttcgagac
    22501 gttcgaggca gaggtagttt gagcgccctt tcagcaacgc cacgttgccc gtgtatttca
    22561 acgcctttga gaccgtcggc aaatcgcggc tatagagctg atcctgcaac gcttttgagc
    22621 cggtcgagat aatgactttc tttttcgccc gcagcgcagg ggccaggtaa gcgtaggttt
    22681 tgcccgtacc ggttcctgct tccaccacca gcggctggcc tttttctatc gcctgggtga
    22741 cggctaccgc catctgtcgc tgtggttctc gcggcttaaa gcctggtatc gctttcgcca
    22801 gctgaccgtc tggtgcaaaa tcgtccgtca cactaccccc tgttgatttg aacagggatt
    22861 atgtcaggat gagggcgctt tcgccagttg aagtggtgac ggcgacctca cattgtggca
    22921 gtctttgcag cacgaaaatg gaaggataat gaggataaaa tgatgactat cgttcgtatc
    22981 gatgctgaag cccgctggtc tgatgtagta atccacaaca acacgctcta ctacactggt
    23041 gtaccggaaa acctcgacgc cgatgccttt gagcaaaccg ccaacaccct ggcgcagatt
    23101 gacgccgtgc tggaaaaaca gggcagcaat aaatcgagca ttctggatgc caccattttc
    23161 ctggccgata aaaacgactt cgcggcgatg aataaagcgt gggatgcctg ggttgtcgcg
    23221 ggtcatgcgc cggtgcgctg cacggtacaa gcgggtttga tgaacccgaa gtataaagtt
    23281 gaaattaaga ttgtggctgc ggtgtaagct ttatcgaagc aaaataagtc agacgataat
    23341 ttatcgataa tactggtcgg ttatacataa at
//
LOCUS       DAZFBR010000078        22907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFBR010000078 DAZFBR010000000
VERSION     DAZFBR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.078"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            20..775
                     /gene="repE"
                     /locus_tag="ACHJZ2_003243"
     CDS             20..775
                     /gene="repE"
                     /locus_tag="ACHJZ2_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HFO4569309.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVVKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMHLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            1363..2529
                     /gene="sopA"
                     /locus_tag="ACHJZ2_003244"
     CDS             1363..2529
                     /gene="sopA"
                     /locus_tag="ACHJZ2_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFO4569310.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            2529..3500
                     /locus_tag="ACHJZ2_003245"
     CDS             2529..3500
                     /locus_tag="ACHJZ2_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFO4569311.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            4195..5097
                     /locus_tag="ACHJZ2_003246"
     CDS             4195..5097
                     /locus_tag="ACHJZ2_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO4569312.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLSTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAVLSRRFSCMLEHWYAGQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            5101..5406
                     /locus_tag="ACHJZ2_003247"
     CDS             5101..5406
                     /locus_tag="ACHJZ2_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569313.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            5483..6166
                     /locus_tag="ACHJZ2_003248"
     CDS             5483..6166
                     /locus_tag="ACHJZ2_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO4569314.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            6167..6388
                     /locus_tag="ACHJZ2_003249"
     CDS             6167..6388
                     /locus_tag="ACHJZ2_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569315.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            6282..6836
                     /locus_tag="ACHJZ2_003250"
     CDS             6282..6836
                     /locus_tag="ACHJZ2_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO4569316.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            6882..7658
                     /locus_tag="ACHJZ2_003251"
     CDS             6882..7658
                     /locus_tag="ACHJZ2_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569317.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            8076..8501
                     /locus_tag="ACHJZ2_003252"
     CDS             8076..8501
                     /locus_tag="ACHJZ2_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4569318.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            8548..8970
                     /locus_tag="ACHJZ2_003253"
     CDS             8548..8970
                     /locus_tag="ACHJZ2_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO4569319.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            8967..9158
                     /locus_tag="ACHJZ2_003254"
     CDS             8967..9158
                     /locus_tag="ACHJZ2_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569320.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(9128..9637)
                     /locus_tag="ACHJZ2_003255"
     CDS             complement(9128..9637)
                     /locus_tag="ACHJZ2_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569321.1"
                     /translation="MSRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDRGCATGT
                     VAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKRKKKKNS
                     NRHGNHRKITSEKNIPDTSQTGRVTDDASAWPASLPAGVSLPGVFARADRRPPPPALY
                     HLFLLPCKM"
     gene            10154..10384
                     /locus_tag="ACHJZ2_003256"
     CDS             10154..10384
                     /locus_tag="ACHJZ2_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569322.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            10436..11797
                     /locus_tag="ACHJZ2_003257"
     CDS             10436..11797
                     /locus_tag="ACHJZ2_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO4569323.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAEQFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            11844..12407
                     /locus_tag="ACHJZ2_003258"
     CDS             11844..12407
                     /locus_tag="ACHJZ2_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO4569324.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(12493..12930)
                     /locus_tag="ACHJZ2_003259"
     CDS             complement(12493..12930)
                     /locus_tag="ACHJZ2_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569325.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASPGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHKQENNKTDSNQSSR
                     KKKNSSSSSSSSMNNNIISNRQMTGLKRQKPTAECPRNVRGGA"
     gene            13000..13206
                     /locus_tag="ACHJZ2_003260"
     CDS             13000..13206
                     /locus_tag="ACHJZ2_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4569326.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            13232..13684
                     /locus_tag="ACHJZ2_003261"
     CDS             13232..13684
                     /locus_tag="ACHJZ2_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4569327.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            13741..13974
                     /locus_tag="ACHJZ2_003262"
     CDS             13741..13974
                     /locus_tag="ACHJZ2_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO4569328.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            14040..15998
                     /locus_tag="ACHJZ2_003263"
     CDS             14040..15998
                     /locus_tag="ACHJZ2_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO4569329.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            16053..16487
                     /gene="psiB"
                     /locus_tag="ACHJZ2_003264"
     CDS             16053..16487
                     /gene="psiB"
                     /locus_tag="ACHJZ2_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO4569330.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            16484..17245
                     /locus_tag="ACHJZ2_003265"
     CDS             16484..17245
                     /locus_tag="ACHJZ2_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO4569331.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            17419..17568
                     /locus_tag="ACHJZ2_003266"
                     /pseudo
     CDS             17419..17568
                     /locus_tag="ACHJZ2_003266"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            17510..17635
                     /locus_tag="ACHJZ2_003267"
     CDS             17510..17635
                     /locus_tag="ACHJZ2_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO4569332.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            17769..19790
                     /locus_tag="ACHJZ2_003268"
                     /pseudo
     CDS             17769..19790
                     /locus_tag="ACHJZ2_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            19858..20289
                     /locus_tag="ACHJZ2_003269"
     CDS             19858..20289
                     /locus_tag="ACHJZ2_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO4569333.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(20933..21282)
                     /locus_tag="ACHJZ2_003270"
                     /pseudo
     CDS             complement(20933..21282)
                     /locus_tag="ACHJZ2_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            21376..21639
                     /locus_tag="ACHJZ2_003271"
                     /pseudo
     CDS             21376..21639
                     /locus_tag="ACHJZ2_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            21679..21799
                     /locus_tag="ACHJZ2_003272"
                     /pseudo
     CDS             21679..21799
                     /locus_tag="ACHJZ2_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            21796..21948
                     /locus_tag="ACHJZ2_003273"
                     /pseudo
     CDS             21796..21948
                     /locus_tag="ACHJZ2_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            22055..>22907
                     /locus_tag="ACHJZ2_003274"
     CDS             22055..>22907
                     /locus_tag="ACHJZ2_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFO4569334.1"
                     /translation="MIHPGSSLDKAINNTRVKNVSTDVKHGQIQERKRNFIYKKNDDI
                     SSRFKLYSSLVKQKNATEDVVLIGKMILDEVRSYRTIHNDRNIVSNSGNWKTSFLCNL
                     ARLLYSIFNGSNYFCSREGENNSSPSSTLLTIHQPEKQELLQQKSIKHLPTSNNIDGY
                     IKIRKTRGAEDQTTTITQSLIINELLNGVDRNTIPFQKISELNDIIHSYENMQIKNSR
                     KGIEILVKQGELLSSLINDNKGNKQLSDNASKIINLLGIEYQSHKVDIEPFIHAVWVA
                     GAPPDNTF"
BASE COUNT         5432 a         5656 c         6514 g         5305 t
ORIGIN      
        1 cacttcacat ggatctgtca tggcggaaac agcggttatc aatcacaaga aacgtaaaaa
       61 tagcccgcga atcgtccagt caaacgacct cactgaggcg gcatatagtc tctcccggga
      121 tcaaaaacgt atgctgtatc tgttcgttga ccagatcaga aaatctgatg gcaccctaca
      181 ggaacatgac ggtatctgcg agatccatgt tgttaaatat gctgaaatat tcggattgac
      241 ctctgcggaa gctagtaagg atatacggca ggcattgaag agttttgcgg ggaaggaagt
      301 ggttttttat cgccctgaag aggatgccgg cgatgaaaaa ggctatgaat cttttccctg
      361 gtttatcaaa cgtgcgcaca gtccatccag agggctttac agtgtacata tcaacccata
      421 tctcattccc ttctttatcg ggttacagaa ccggtttacg cagtttcggc ttagtgaaac
      481 aaaagaaatc accaatccgt atgccatgca tttatacgaa tccctgtgtc agtaccgtaa
      541 gccggatggc tcaggcatcg tctctctgaa aattgactgg atcatagagc gttaccagct
      601 gcctcaaagt taccagcgta tgcctgactt ccgccgccgc ttcctgcagg tctgtgttaa
      661 tgagatcaac agcagaactc caatgcgcct ctcatacatt gagaaaaaga aaggccgcca
      721 gacgactcat atcgtatttt ccttccgcga tatcacttcc atgacgacag gatagtctga
      781 gggttatctg tcacagattt gagggtggtt cgtcacattt gttctgacct actgagggta
      841 atttgtcaca gttttgctgt ttccttcagc ctgcatggat tttctcatac tttttgaact
      901 gtaattttta aggaagccaa atttgagggc agtttgtcac agttgatttc cttctctttc
      961 ccttcgtcat gtgacctgat atcgggggtt agttcgtcat cattgatgag ggttgattat
     1021 cacagtttat tactctgaat tggctatccg cgtgtgtacc tctacctgga gtttttccca
     1081 cggtggatat ttcttcttgc gctgagcgta agagctatct gacagaacag ttcttctttg
     1141 cttcctcgcc agttcgctcg ctatgctcgg ttacacggct gcggcgagcg ctagtgataa
     1201 taagtgactg aggtatgtgc tcttcttatc tccttttgta gtgttgctct tattttaaac
     1261 aactttgcgg ttttttgatg actttgcgat tttgttgttg ctttgcagta aattgcaaga
     1321 tttaataaaa aaatgcaaag caatgattaa aggatgttca gaatgaaact catggaaaca
     1381 cttaaccagt gcataaacgc tggtcatgaa atgacgaagg ctatcgccat tgcacagttt
     1441 aatgatgaca gcccggaagc gaggaaaata acccggcgct ggagaatagg tgaagcagcg
     1501 gatttagttg gggtttcttc tcaggctatc agagatgccg agaaagcagg gcgactaccg
     1561 cacccggata tggaaattcg aggacgggtt gagcaacgtg ttggttatac aattgaacaa
     1621 attaatcata tgcgtgatgt gtttggtacg cgattgcgac gtgctgaaga cgtatttcca
     1681 ccggtgatcg gggttgctgc ccataaaggt ggcgtttaca aaacctcagt ttctgttcat
     1741 cttgctcagg atctggctct gaaggggcta cgtgttttgc tcgtggaagg taacgacccc
     1801 cagggaacag cctcaatgta tcacggatgg gtaccagatc ttcatattca tgcagaagac
     1861 actctcctgc ctttctatct tggggaaaag gacgatgtca cttatgcaat aaagcccact
     1921 tgctggccgg ggcttgacat tattccttcc tgtctggctc tgcaccgtat tgaaactgag
     1981 ttaatgggca aatttgatga aggtaaactg cccaccgatc cacacctgat gctccgactg
     2041 gccattgaaa ctgttgctca tgactatgat gtcatagtta ttgacagcgc gcctaacctg
     2101 ggtatcggca cgattaatgt cgtatgtgct gctgatgtgc tgattgttcc cactcctgct
     2161 gagttgtttg actacacctc cgcactgcag tttttcgata tgcttcgtga tctgctcaag
     2221 aacgttgatc ttaaagggtt cgagcctgat gtacgtattt tgcttaccaa atacagcaat
     2281 agtaatggct ctcagtcccc gtggatggag gagcaaattc gggatgcctg gggaagcatg
     2341 gttctaaaaa atgttgtacg tgaaacggat gaagttggta aaggtcagat ccggatgaga
     2401 actgtttttg aacaggccat tgatcaacgc tcttcaactg gtgcctggag aaatgctctt
     2461 tctatttggg aacctgtctg caatgaaatt ttcgatcgtc tgattaaacc acgctgggag
     2521 attagataat gaagcgtgcg cctgttattc caaaacatac gctcaatact caaccggttg
     2581 aagatacttc gttatcgaca ccagctgccc cgatggtgga ttcgttaatt gcgcgcgtag
     2641 gagtaatggc tcgcggtaat gccattactt tgcctgtatg tggtcgggat gtgaagttta
     2701 ctcttgaagt gctccggggt gatagtgttg agaagacctc tcgggtatgg tcaggtaatg
     2761 aacgtgacca ggagctgctt actgaggacg cactggatga tctcatccct tcttttctac
     2821 tgactggtca acagacaccg gcgttcggtc gaagagtatc tggtgtcata gaaattgccg
     2881 atgggagtcg ccgtcgtaaa gctgctgcac ttaccgaaag tgattatcgt gttctggttg
     2941 gcgagctgga tgatgagcag atggctgcat tatccagatt gggtaacgat tatcgcccaa
     3001 caagtgctta tgaacgtggt cagcgttatg caagccgatt gcagaatgaa tttgctggaa
     3061 atatttctgc gctggctgat gcggaaaata tttcacgtaa gattattacc cgctgtatca
     3121 acacagccaa attgcctaaa tcagttgttg ctcttttttc tcaccccggt gaactatctg
     3181 cccggtcagg tgatgcactt caaaaagcct ttacagataa agaggaatta cttaagcagc
     3241 aggcatctaa ccttcatgag cagaaaaaag ctggggtgat atttgaagct gaagaagtta
     3301 tcactctttt aacttctgtg cttaaaacgt catctgcatc aagaactagt ttaagctcac
     3361 gacatcagtt tgctcctgga gcgacagtat tgtataaggg cgataaaatg gtgcttaacc
     3421 tggacaggtc tcgtgttcca actgagtgta tagagaaaat tgaggccatt cttaaggaac
     3481 ttgaaaagcc agcaccctga tgcgaccacg ttttagtcta cgtttatctg tctttactta
     3541 atgtcctttg ttacaggcca gaaagcataa ctggcctgaa tattctctct gggcccactg
     3601 ttccacttgt atcgtcggtc tgataatcag actgggacca cggtcccact cgtatcgtcg
     3661 gtctgattat tagtctggga ccacggtccc actcgtgttg tcggtctgat tatcggtctg
     3721 ggaccacggt cccactcgtg ttgtcggtct gattatcggt ctgggaccac ggtcccactt
     3781 gtattgtcga tcagactatc agcgtgagac tacgattcta tcaatgcctg tcaagggcaa
     3841 gtattgacat gtcgtcgtaa cctgtagaac ggagtaacct cggtgtgcgg ttgtatgcct
     3901 gctgtggatt gctgctgtgt cctgcttatc cacaacattt tgcgcacggt tatgtggaca
     3961 aaatacctgg ttacccaggc cgtgccggca cgttaaccgg gctgcatccg atgcaagtgt
     4021 gtcgctgtcg ccggcctcct cacccggtca cgtttcgtcg ttcctcctcc acgcgctgcg
     4081 gcttcggggc cgcacctgca ttcgtatgcg gtcgcccggt tacaggtgcg gcacggcctg
     4141 atggaggccg catgtgagag gagaattccc atgccaaact ggtgctcgaa tcgtatgtat
     4201 ttttctggtg aaccggcaca gatcgctgag attaaacgac tggccagcgg tgcagtcaca
     4261 ccgctttatc gccgcgccac aaatgaaggt attcagctgt ttctggccgg aagtgccgga
     4321 cttctgcaga ccactgaaga tgtgcggttt gaaccatgcc ccggactgac ggctgccggg
     4381 cgtggcgttg tatcgccgga gaatatcgcg ttcacccgct ggctgacaca cctgcaggac
     4441 ggtgtacttc tggatgagca aaactgcctg atgctgcatg aactctggct gcagagcggt
     4501 actggccggc gtcgctggga agaattaccg gatgatgcca gggaaagcat taccgctctt
     4561 ttcaccccaa aaagaggtga ctggtgcgac atctggagta acgaggatgt atctgtgtgg
     4621 tggaaccgtc tgtgtgacaa cgtcctgccg gaaaaaacca tgccgtttga cctgctgacg
     4681 gttctgtcca ctcgcctgga tgttgaagtg aatggcttta acggtggtgt tctgaacggt
     4741 gttccttctg catatcactg gtatacggaa cagtatggcg tgaagtggcc ttgtgggtat
     4801 gaggtgaata tcagcagtca gggagacaac ttcattcagg ttgatttcga cacgccgtgg
     4861 tgtcagccgg aaagcgacgt tattgcagta ttaagccgcc gtttcagttg catgctggag
     4921 cactggtacg ccgggcaggg ctgcaatttc tgtggctggc agcgatatga gcgtggagag
     4981 cttgttgatg tgctgtgggg ggaactggaa tggtcttccc cgacagatga tgatgagctg
     5041 ccggaagtca ccgcgccgga gtggatagtc gacaaggtgg cgcattacgg tggatgaagt
     5101 atgacggaga cgggcgggca accgcccgtt tcttttccgg taaaggatgt cgccgggctc
     5161 ctctttttac tgcgccggct gacgcggcgc ggcaggaacg ctgcctgtgg tcagtgtccc
     5221 gcgtccggcc gggcacggga cgggcgcttt taccgctccc ggctggcatc ggtgacagtg
     5281 tacgccagcc cgtcgccctt ttctgatgaa cgcccttcaa gccgctttcg cggcatattc
     5341 tcgccgtcaa aaagacgacg gctgcgatat tccacggtcg gcctgacccg ttaccggacg
     5401 cggtgaacag cccacaggcc gcggggaacg ggcgccacag gggcgccctc ccggtgccct
     5461 ctgtaagaga aggagtgctg ttatgtcccg ttttatccag ggtgactgcg tgcgtgttat
     5521 ggccacgatt ccgggcaatg gtgtcgattt catcctcacc gacccgccat atctcgtcgg
     5581 tttccgtgac cgtcaggggc gtaccatcgc cggcgataaa accgatgaat ggctgcaacc
     5641 ggcctgtaat gaaatgtacc gcgtgctgaa aaaagatgcg ctgatggtga gcttctatgg
     5701 ctggaaccgc gtcgatcgct ttatggccgc ctggaaaaat gcgggattca gcgtggttgg
     5761 ccacctggtg tttaccaaaa cctacacttc gaaggccgca tatgtgggct atcgccacga
     5821 atgcgcttac atcctggcaa aagggcgtcc ggcgctgcct cagaagccat tgcccgacgt
     5881 gctgggctgg aaatattcag gcaatcgtca tcacccgacg gaaaagcctg ttaccagcct
     5941 gcaaccactg attgagagct tcacacaccc gaacgcgatt gtgctggacc cgtttgcagg
     6001 cagcggctca acctgtgtgg ccgcccttca gtccggacgc cggtatatcg gtatcgagct
     6061 gcttgaacag tatcaccgtg ccggacagca acgtctggct gccgtacagc gggccatgca
     6121 gcagggggcc gcaaatgatg actggtttat gccggaggct gcgtaaatga actatgccgg
     6181 acatgaaaaa cttcgcgccg acgtggcgga ggtggccaac accatgtgtg accttcgggc
     6241 caggctgaac gatatggagc atcgctgtcg ctttgattcc gatgtgcttg ttgaacgcct
     6301 ggcacgccag acgctgtatc gcgccaatcg cctgttcatg gaggcgtata ccgaaattct
     6361 ggaactggat gcgtgcttta aagattgaga aggagacgag aatgtacgga acatgcgaaa
     6421 cgctatgccg ggagctggca gcaaagtatc cgggatatac accgctgatg ctggttatct
     6481 ggtccccgga agagattcag gccctcgctg acggaatgga tatttccctg tccgatcctg
     6541 aaatcaggac cgtcctggcg cgcctggagg acatcccgga agaccagcga attgagtccg
     6601 gtatttcttc tgccgcggcg atggagatta tcagcaacgt gagcgaaaac cgccaggtga
     6661 ccgtcccggc tgaactgctg gcgtccctga ttcagaccgc cgaacaggca ctgtggaaac
     6721 gtgaatgggc cgcccgtgat tacggtctcg ccgtcccgga atgcgttacc cgccgtcagg
     6781 cggtggttaa tcaggcccgc atcctgctga aaaacaacac acacgaaaac ggctgatgtt
     6841 atcgccgcct gcgggcggcg atacagggag aacgattaat catgaacgaa aacacaacgc
     6901 tgaacgcact gatttgtcgt cacgcccgta acctgctgct ggcgcagggc tggccggaag
     6961 agacggatgt cgaccagcgt aacccgaagt atccgggctg gatcagcatt tatgttctgc
     7021 tggatgcgcc ccggctggcg acgttactcg ttaaccgcca cggcggcgta ctgccgccgc
     7081 acctggcctc cgccattcag aaactgaccg gaaccggagc ggaacttgtt ctgtccggca
     7141 gtcagtggca gtcgctgccg gtgcttccgg cagacggaac gcaggtgtct tttccgtatg
     7201 ccggtgaatg gctgaccgaa gacgaaatca gggctgtact tgcggcggtg cgcgatgcgg
     7261 tacgcagtgt cagttgccgg gtggcagaag atacgcggcg tatccgggcg gcgctgacca
     7321 ccaccggtca gacgttactg acccgccaga cgcgccgctt tcgcctggtc gtgaaggaaa
     7381 gcgatcaccc ctgctggctc gatgaggacg acgaaaacct gcccgtggtg ctcgatgcca
     7441 tcctgaaccg gggcgcacgt ttttcggcgg tggaaatgta tctggtcagc gattgtattg
     7501 aacatatcct gtccagtggg ctggcctgcg atgtgctgcg tataccggat gaaccgcccc
     7561 gccggtggtt tgaccgtggt gttctgcggg aggtggtccg ggaagcccgg aacgaaatcc
     7621 gcagcatggc ggatgccctg gcaaaaatcc ggaaatgatg acgttggcgg ggtaaatccc
     7681 cgcctttttc ctgccggtcc ccggacagag cacaaaggaa gtcgccgctg gctccgcttt
     7741 acccggccat gcagggcatg gccttgtggt ttttcagtta tgtggcctca gcgtcgtgtg
     7801 cgggcggtgc cgtgcctcca tcttagccgg gctggcgttg atgcaagggt acgcttcgcc
     7861 gctgcggtca cccggtccct ccttcccgtc tgccgtgatt ttccggtcgt tcgcccgctc
     7921 aggtttcgcg gcctcaccct gcaactcccg tcagccgtgt gcgtcaggct gcggcttccc
     7981 ttgcgccctg tgcatccccg ccttatcgcc cggcttttct ggaggcacgg caccgcccgg
     8041 tgtcgcggaa tatgtgaaca acggaggaaa acatcatgca atatgcgaaa cctgtcactc
     8101 tgaatgttga agagtgcgac cgtctctcct ttctgcctta cctgtttggc aacgattttc
     8161 tgtatgccga ggcgtatgtg tacgcgctgg cgcaaaaaat gatgccggaa tatcagggag
     8221 gcttctggca cttcatccgc ctgccggacg gtggcggtta catgatgccg gatggcgacc
     8281 gtttccacat ggtgaacggt gcaaactggt ttgaccgtac cgtgagtgct gatgcctgcg
     8341 gcatcattct gacttccctt gtgattaacc gccagttgtg gctgtaccac gacagcggtg
     8401 atgcaggact gacccagctt taccggatgc gcgatgcgca gttgtggcgt cacatcgaat
     8461 ttcaccctga atgcaacgcg atttacgcgg cactggactg attaacggac ggggcggcaa
     8521 tgccgcccct gtaaaggaga cgagaacatg tactgtactg ttaaagaaat catccgcgat
     8581 gtactggata cagacgtgcc ggacagtgaa tgcgtttttg ccgtggtgct gacccgtggg
     8641 gatgtgcgcc acatagccca ggactggaac ctgtcagacg atgagctgga aaccgtcatg
     8701 cagcggctgg acgatgcctt tgaatatggt gcggatgtca gcattgttca cgacgttgtt
     8761 cgtgagttga tggaagaaaa gcgtgccagc cgtcaggtga cagttccggc ggtgatgctg
     8821 gaaaaagtga tggcgctggc aggcagtgaa atgaagcgcc tgtatgccgt cgggagtgag
     8881 aacgggggcg acggtgatgc gttcgtcagg gaggaacgcg aagcaatgga cgttgtgtta
     8941 caggcgctgg acggggagca catgtcatga atatcagcac agaaacccgc gaaattctgc
     9001 gcaattacaa agccgtgatt aatgcgcgac gtcgtgaaat ggggcagaaa ccgctcacca
     9061 ccgcgcagat tgttgatgaa atctgcgatt ttgtggcgaa tcagcaggct gttttcctgg
     9121 gcggtcacta catcttacag ggcagcagaa acaggtgata cagggctggc ggtggaggcc
     9181 gcctgtcagc ccgcgcgaaa acccctggca ggctgacgcc tgccggaagg gaagcgggcc
     9241 acgctgacgc gtcgtccgtt acccttcctg tttgtgatgt gtccggaatg tttttttctg
     9301 atgttatttt tctgtggtta ccgtgcctgt tactgttctt cttctttttc ctcttcctct
     9361 tcctcctctt ctttccgttc ctctcctgct tatggctttt ccgggcgtcc ccctggcggg
     9421 gcgggctgtc gtgaacggag ccagcgaagc tgtctccgcc ctggcgggct tccatccctg
     9481 acgcgaaacc ctggcttgca gcgaccgttc cggtcgcgca gcctctgtcg cgccgtggcc
     9541 tgcgctcccg gcatcccggc aaaaggccgc cgggatgtaa ggggcaacgc cgtttcgcaa
     9601 accgcgcttc acgtcgttgc ggcgggtccc tgctcattgc ctgccgggtg ccacacaccc
     9661 gtatgagtcc ctgtcgccgg gagctgatac agccacaacc tgccgcactg actgtccagg
     9721 gtaaatgaac agcccacaaa attttttacc cagaacccgg taaaaaattt tgcgttctgc
     9781 ccaggacagg tgtgtcaggc cgtggcagtg atgccccttg cggcgaaggt tctcaaacgg
     9841 gtgaagttca taccacaggg aatacagcag caggttcccg cgaaacgtca aaaccgcggc
     9901 tcccgaaact caacgggtga tgttctttaa aaatcaggat ttatttcagg ctccggcaat
     9961 acgcatttat gtgttctgtc agtcatccgg ctgacaggta atgagaacgc aggaacagaa
    10021 ggaggaaaac accggaaagc gtcagatgca gatatgaaaa aagcaggtca gggacctgct
    10081 taatccacgc cgtaagaatt gtgcgaccaa ttttgacggc attgttaacc aatttgtcat
    10141 aaggaatcca tttatgactg taagcattgt ttcaccttcc gcagcggctg taaagccccg
    10201 ccgtcatccc cgtttcagac gcgaagacat ccccgctccg gaaattgacc ccgtgctgaa
    10261 agccttcggg cgacacatag cccggagttt tcaccggggg cgtggtgtac acatccccgc
    10321 catgaaaaac acggctttcg ggcaggtgct ccgcacactc gaactgaaac gcgcttttaa
    10381 ctgatgaggt gagccgcccc gcgaggggcg gcaacagacg gaaggagaac attttatgac
    10441 atcagcagca atcaccatga ccgccccgga agccgccagc cctgtgcaga tgtaccgcgc
    10501 gacctactca ccggatgaca acaaactgcg cctgtatgcc gtgtcacgtc ttgacccgga
    10561 gacgtataaa aaagtgcatg atgccggttt tcgctgggca ccaaaacagg cgctgtttgt
    10621 cgcgccggcc tggacaccgg gccgggaaga cgtgctcctc tcacttgccg gagagattga
    10681 ggatgaagac agcacgctcg ctgaacgtca ggaagcacgg gcggagcagt ttaccggata
    10741 cagcggaaag cgggccagtg aatccgcaca ggcacttgat gaagtggaaa gactggccgc
    10801 gatgatcccg cccggtcagc ccattcttgt ggggcatcac agcgaacgcc gcgcccgtcg
    10861 tgatgcgcag cgtattgaaa acggcatgaa acgtgccgtg atgctctttg aacgtgcgga
    10921 atactgggaa gaacgggggc ggtcagcact gcttcacgcg aagtataaag aacgtccgga
    10981 cgttcgctgg cgtcgtatca aaaaaatcga agctgatttg cgcaaggctg aaaagaccat
    11041 cgcgcagtcg cagaaatatc tgacgatgtg gcgggctgaa tcgctggatc tgaatatggc
    11101 aaaactcatc agcagtcatg accatatcag cgcctgtttc ccgctggata cgtatccgcg
    11161 cccggcagaa aaaagccagt atgaagggag ccggtcgtta tggtcggccc tggatgatga
    11221 catcatcacc acggagcagg cccgcgaaat tgcgatccgc tgtcatgaac ggcagattca
    11281 gcatcagcaa cgctgggtta accactatca gaaccgcctg aactatgagc gtgccatgct
    11341 ggacgaaagc ggcggcgtgg ttacccggac acaggatttt gagccgggcg gacaggtttt
    11401 cagccggggc gagtggctga ccatcatccg cgtgaacaaa agcaacgggg cggtgagttc
    11461 agtcacaacg ccgaattaca gttttctcgg gtacagcggc acgatgaaag tgacgcccga
    11521 tcgcatcacg gactacaaag caccatcggc agaagaggct gccgtcgcca gccaggccgc
    11581 gaagcgtccg ccggtagtca actatccggg ggaaggtttc cgggaaatga caaaggcaca
    11641 gtgggccgcc ctgccccggg actgtaaggc cgtgcgcagt gtggcagaag cagaagacca
    11701 cggggcatac cgctaccgcc gcacaatgga caataatttc cgtctggtga atgtgtatat
    11761 caccgacatg aaaattacgg aaatcccaca gaaataaggt atatcccccc gggaaatccc
    11821 cgggggatga cggagataat gacatgcaca gtcagttaaa agaacgtatc cggctgatgc
    11881 gcgcaaggct ggataacgcc gcgccggttg ctgaaatccg ggctgaatct cagctttttg
    11941 tgactcctgc cccggtctgt gatcgcctgg tgacgctggc ggagatcagc aaccgtgatc
    12001 acattctgga accctctgcc ggcaccgggg ccatactgcg ggcgattcgg gataccgcac
    12061 cggaggccat gtgtgatgcg gtggaaatca acagcgggct ggtccggtat ctgcgggaaa
    12121 attttaacgg tgtcagggtt cagtgtggcg acttcatgga atggcagccg gtgcagtatt
    12181 acagccgggt tatcattaat ccgccgttca gccacgggca ggatatccgg catatcctgc
    12241 gggccttttc cctgttgcgt ccgggtggcg tgctggtcgc cgtctgtctg aacgggccgc
    12301 gccagcagga gaagctgtta ccgttttctg acgtccgcga ggagctgcca cgcggcacgt
    12361 ttgcttatac cgatgttccg acgatgatta ttcgtctgcg tgcctgatga ctccgggcag
    12421 gcgggaggat aaatgcccgt ctgcccgcgc cgggaaaaga ccgcgcgccg gtggcgctga
    12481 aaggggccgc gctcacgcgc cgccccttac attacgcgga cattctgctg tgggcttctg
    12541 tcttttcaga cctgtcattt gtctgttact gatgatgttg ttgttcatgc tactgctact
    12601 gctactgcta ctgttcttct tttttctgct gctctgatta ctgtctgttt tgttgttctc
    12661 ctgcttatgc cttttccggg cgtccccctg gcggggcggg ctgtcgtgaa cggagccagc
    12721 ggagctgtct ccgccctggc gggcttccat ccctgacgcg aaacccgggc ttgcagcgac
    12781 cgttccggtc gcgccgcctc tgtcgcgccg tggcctgcgc tcccggcatc ccggcaaaag
    12841 gccgccggga tgtaaggggc aacgccgttt cgcaaaccgc gcttcacgtc gttgcggcgg
    12901 gtgcctgccc gttgttgtgt ccggtgccat tcacctgttt aagcccctgt cgccgggggc
    12961 aaagacagcc acaaccgtct ccgccagtcc aggggtgaaa tgcacatccc gcaaaacttt
    13021 ttgcccaaaa gcaggcaaaa agttttgcgg tctgcccccg gacagtcgtt tccggccgtg
    13081 gcagtgatgc cccttgcggc gaaggttctc aaaaaggtga tggtgaatac cggagggaac
    13141 aaaaggcagg tccccgcaaa acgtcaaaac cgcggctccc gaaactcaaa agatgatggg
    13201 ggctgaaaac atcaacagaa ggagacacat catggcagtt cgtggcatta acaaggtcat
    13261 cctcgtagga cgcctgggaa aagatccgga agtccgttac atccccaacg ggggcgcagt
    13321 ggcaaacctg caggtggcca cgtcagaaag ctggcgtgac aaacagacgg gggagatgcg
    13381 ggagcagaca gaatggcatc gcgtggtgct gttcggcaag ctcgcggaag tggcaggtga
    13441 atatctgcgc aagggcgcgc aggtctacat cgaaggtcag cttcgcaccc gtagctggga
    13501 ggataacggt atcacccgtt acgtcaccga aattcttgtt aagaccacgg gcaccatgca
    13561 gatgctggta cgtgccgcag gtgctcagac tcagccggaa gaggggcaac agttcagcgg
    13621 tcagcctcag ccgcaaccgc cggagggtga cgattacggg ttttcagacg atatcccgtt
    13681 ctgaacgact gactgtgaca accgccccgt cctgtgcggg gcatcaccgg agagatgagg
    13741 atgagcgaat atttcagaat acttcaggga ctgcctgacg gtccttttac ccgcaaacat
    13801 gccgaagccg ttgccgcaca gtaccggaac gtctttatcg agaatgacca cggcgaacag
    13861 tttcgcctgg ttgtccgtaa taacggtgca atggtctggc gtacctggaa ttttgaggac
    13921 ggtgccgggt actggatgaa ccatgtcatc cgtgatttcg ggattattaa gtaagagagg
    13981 tgccggacgg gaacgcgtcc ggcagacaat cattattacc gttttgggag gtactaacta
    14041 tgtcagttac agagtctaag gcaaaaacgg agcgtaaatc cagccgtaaa cctgcaaaaa
    14101 cgcaggaaac agtcctgtcg gccctgctgg cgcagacgga ggaagtgagc gtgccgctgg
    14161 cctcgctgat taagtcaccg ctgaatgtgc gcacggtgcc gtattctgcg gagtccgtca
    14221 gcgaactggc ggactccatt aaaggtgtcg gcctgttaca gaatctggtt gttcatgccc
    14281 tgccaggtga ccgttacggt gtcgccgcag gtggtcgccg actggcagca ctcaacatgc
    14341 tggcagagcg tgacatcatt ccggctgact ggcctgtccg tgtgaaggtc attccgcagg
    14401 agctggcgac tgccgcatcg atgaccgaga acggtcatcg tcgggatatg caccctgccg
    14461 aacagattgc cggattccgt gcaatggcgc aggaaggcaa aacacctgca caaatcggtg
    14521 atttgctggg ttattcgccc cgccacgttc agcgaatgct gaaactggca gaccttgcgc
    14581 ctgtcatcct cgatgcgctg gcagaagacc gcatcaccac cgagcactgt caggcgctgg
    14641 cgctggagaa cgacaccgcg cgtcaggtgc aggtgtttga agccgcctgt cagtcgggat
    14701 ggggcggtaa accggaagta cagaccattc gtcgtctggt gaccgaaagt gaagtggcgg
    14761 tggcagggaa cagtaaattc cgcttcgtgg gggctgatac cttctcgcca gatgaactgc
    14821 gcaccgattt gttcagtgac gacgagggtg gctatgtgga ctgcgtggcg ctcgatgccg
    14881 ccctgctgga aaaactccag gctgtcgccg aacaccttcg ggaagccgaa ggctgggaat
    14941 ggtgcgccgg acgtatggag cctgttggtt tctgccgtga ggatgccgga acataccgca
    15001 gtctgccgga gccggaagcg gtgctgacgg aggcagaaga agaacgcctg aacgaactga
    15061 tggcacgtta cgatgcgctg gaaaaccagt gtgaggaatc cgacctgctg gaagccgaaa
    15121 tgaagctgat gcgctgcatg gcgaaggtca gggcgtggac gccggagatg cgtgccggaa
    15181 gtggcgtggt ggtgtcctgg cgttatggca acgtgtgtgt ccagcgtggt gtgcagttgc
    15241 gcagtgagga tgatgtggct gataacgatt accgcacgga acaggtgcag gagaaagcct
    15301 cagtggagga aatcagtctg ccgctgctga cgaaaatgtc ctcagagcgc acgctggcag
    15361 tccaggcggc actgatgcag caatcagaca aatccctggc acttctggca tggacactct
    15421 gtctgaatgt atttggcagt ggagcgtaca gcaatcccgc cagaatccgc ctggaatgtg
    15481 agcattattc gctgaccagc gatgcgccgt cagggaagga aggtgccgca ttcatggcga
    15541 tgatggcaga aaaagcccgt cttgccgccc tgctgccgga tggatgggca cgggatatga
    15601 cgacgttcct gtcactcagt caggaggtgc tgttatcact gctcagtttc tgcaccgcgt
    15661 gcagtatcca cggtgtccag acccgtgagt atggtcacac gtcacgcagt ccgctggaca
    15721 cgctggagag cgccatcggc tttcacatgc gcgactggtg gcagccgacg aaagcaaact
    15781 ttttcggaca cctgaaaaag ccgcagatta tcgcagccct gaatgaggca ggactatccg
    15841 gtgccgcacg ggacgcggag aagatgaaga aaggcgatgc ggctgaacat gcagagcacc
    15901 atatgaaaga caaccgctgg gtgcctggct ggatgtgtgc accacgccca cagacggatg
    15961 ccactgaacg caccgataac ctggctgatg ccgcctgatg aacaaccaca ccgccccgcc
    16021 ggagacgggg cggcagcaag ggagataccg tgatgaaaac tgaactgact ctgaatgtat
    16081 tacataccat gaacgcacag gaatatgaag atatccgggc tgccggaagt gatgcacgcc
    16141 gtgagctgac acacgccgtg atgcgggagc tggatgcgcc ggacaactgg acgatgaacg
    16201 gcgagtacgg cagcgagttt ggcggctttt tccccgtcca ggtccgtttc acgcctgccc
    16261 acgaacgttt tcacctggcg ttatgttcac cgggcgatgt ctcccaggtc tgggtgctgg
    16321 ttctggtgaa tgccggtggt gagccgtttg cggtggttca ggtacagcgg cggtttgcac
    16381 cggaagccgt cagccattca ctggcactgg cggcgtcact ggatacgcag ggatacagcg
    16441 ttaacgacat catccatatc ctgatggcgg aaggaggtca ggtatgagcg cacgttcaca
    16501 ggcactggtt cccctcagca cagagcaaca ggccgcatgg cgggcagtgg cagagacgga
    16561 aaaacgccgc aatcagggaa acacgctggc ggaatatccg tatgcggggg cgtttttccg
    16621 ctgtctgaac ggaagccgca ggatatcgct gtctgacctg cgatttttca tgccgtccct
    16681 gaccgcagaa gaactgcacg gaaaccgtct tcagtggttg tatgccatcg atgtgctgat
    16741 tgaaacacag ggcgaggtct gcctccttcc ccttcccggt gatgcagcag aacgcttgtt
    16801 cccgtcggtg cggttttgtg tcagggaacg cagtcgccat aaatccgcgc tggtcatgca
    16861 gaagtacagc cgccagcagg cgagggaggc agagcagaaa gcccgtgcgt accaggcgct
    16921 ggtcgcccag gctgaaatcg aactggcgtt ccattcaccg gaaacagtcg gaagctggta
    16981 tgcacgctgg agtgatcggg tggcagagca tgacctggaa accctgttct ggcagtgggg
    17041 cgagcgtttt ccgtcactgg ccggaatgga acgctggcag tggcaggata tgccattctg
    17101 gcaggttatc gcggaagcca gtctggcagc aaaggaggcc ggtcatgctg tccgtgagat
    17161 ggagcggtgg atggtgccga acaaactccg ggaggtgcgt gatgcggcaa caatcacacg
    17221 gattgcccgt aaacagcctg aatgagcggg ttattttcag gaaaagtgaa tgtggtcagc
    17281 gtgctggtat atgggctatg atgtgcccgg cgcttgaggc tttctgcctc atgacgtgaa
    17341 ggtggtttgt taccgtgttg tgtggcagaa ggacaaaagc cccgtagtta atttttcatt
    17401 aacccacgag gcccctgcat gcttagacaa catcaggata gcctcttacc gcgctttgcg
    17461 caaggagaag aaggccatga aactaccacg cagctctctt gtctggtgtg tgttgatagt
    17521 gtgtctcaca ctgttgatat tcacttatct gacacgaaaa tcgctgtgcg agattcgtta
    17581 cagagacgga tacagggagg tggcggcttt catggcttac gaatccggta agtagcaacc
    17641 tggaggcggg cgcaggcccg ccttttcagg gctgatgctg gtctgactgc actgaagcgc
    17701 ctttataaag gggctgctgg ttcgccggtg gcccctttct ccttgctggc attgtacggg
    17761 catgaacagc ctggcgacgc gggaaaaagc agacgttcag ggaatatatt gctcagatgt
    17821 cgattgccgg gctgcgtgaa gagcccgcac aggactggtt atctttgcat aaacggctgg
    17881 ccagtgatgg tctgtatatc acgatgcagg aaggggaact ggtggtgaag gatggctggg
    17941 acagagcgcg ggaaggtgtt gcactcagtt cgttcggacc gttatggaca gctgaaaaac
    18001 tggggcggaa actgggggaa tatcagcctg taccgacaga tattttcagt caggtgggga
    18061 cacccggtcg ttatgatccg gaagccataa acgttgatat ccggccggaa aaagtggcag
    18121 aaacggagag tctgaaacag tacgcctgcc gtcattttgc cgaacgctta ccggaaatgg
    18181 cgagaaatgg tgagctggaa agttgcctgg atgtgcacag aactctggca acggctggat
    18241 tatggatggg gatccagcac gggcatctgg tactgcatga tggatttgat aaacaacaga
    18301 caccagtacg tgcagacagt gtgtggccgt taatgacgct ggattatatg caggatctgg
    18361 atggtggatg gcagcctgta ccgaaggata tttttactca ggttataccc ggagaacggt
    18421 tccggggacg taatcttggt actcaggctg tcagtgatta cgaatggtat cgtatgcgga
    18481 tggggacagg tccacaggga gcaataaagc gcgaactgtt ttctgataag gaaagtctgt
    18541 gggggtacac aactgttcag tgtgagtcgt tgattgaaga tatgatcgcg ggcgggaatt
    18601 tcagctggca ggcgtgccat gagatgtttg cgcgtaaagg gcttatgttg cagaaacagc
    18661 atcatggtct tgtgattgtg gatgcgttta atcatgagct cacaccagtg aaggccagca
    18721 gtatccaccc ggatttgacg ttatccaggg ctgagccgca ggcagggcca tttgagattg
    18781 caggtgcaga tatatttgaa cgggtgaagc ctgaatgtcg ctacaatccg gaacttgctg
    18841 ccagcgatga agtggagccc ggcttccggc gcgatcctgt actgcgtcgt gaacgccgtg
    18901 aagcccgtgc agctgcgcgg gaagatttgc gcgcgcgtta tcttgcatgg aaggagcact
    18961 ggcgtaaacc cgacttacgt tacggagaac ggttacggga aattcatgct gcctgccgtc
    19021 ggcgcaaggc gtatatccgt gtgcagttcc gggatccaca attgcgtaag ctgcattacc
    19081 atattgcgga agtgcagcgt atgcaggcgc tgatcaggct gaaggagagt gtgaaggaag
    19141 agcggttgtc actgattgag gagggtaagt ggtatccttt gtcctaccgg cagtgggtgg
    19201 agcaacaggc ggtacaggga gacagagctg cattatcaca gttacgtggc tgggattatc
    19261 gtgatcgtcg caaagacaag cgacgaacaa cgaatgccga ccgctgcgtg attctctgtg
    19321 aaccgggagg aactccactt tatgaagaca ctggcgttct cgaagcccgt ctgcagaaag
    19381 atggcagtgt gcgttttcgt gatcggagga atggcgagtt agtctgtgtg gattatgggg
    19441 accgtgtggt gttctaccat catcaggaca gaaatgaact ggtggataag ctgaatctga
    19501 ttgctcctgt attgtttgat cgtgagccag gaatgggttt tgaaccggaa ggctcatatc
    19561 aacagtttaa tgatgtattt gcggaaatgg tggcctggca taatgcagct ggaataaccg
    19621 gaaatggaca ctttgtaatc agccgcccgg atgtggattt acatcgtcag cgtagtgaac
    19681 agtactacca cgaatatatc aggcaacaga aaagcatatc cggggggcat ggcgcatctt
    19741 atgctccagt tcaggataat gagtggacgc caccatcacc tggaatgtag ggatgaggag
    19801 tggatattaa aaatcttaac ttgtattagt taagattttc tttgctctga cagagctatg
    19861 gtactgagat ctatatgggt tgaggagcaa tatatggggg taaagaaaat cgttatactg
    19921 gctattttag tcagctttgt ggcgggatgt gcgccattac atccttcaaa ttgccataaa
    19981 actacagccc tgggtagttg cagttcagga cgttgggatg atcaggatga atggggggag
    20041 caggcacggg gaatcaggga tgcaattaat gctaaacttg atgatccgca aaaatggaaa
    20101 aggaaaaaat gcaggttgca tatggaattc tcccgggatg gcacggcgtt aaaaatatca
    20161 accagtaatg gcgataaagc ctattgcgaa gcgataaagt cagcagccca taaagctaaa
    20221 ttcccggcct tcaacaatcc ggaagtttat agagattttc aaaaatccgg ctttgacatg
    20281 cgcggttagc tacacgacat atctgtaggg ctaacagaag actgtcaggt cggctgatta
    20341 ctggaacctg aggtactttg cctcaggtta tgttgacata ctcaaaaaaa gattcttaca
    20401 ttatcccggt aacggcgtcc ttcatcggcc tgtccgttaa actcaggcta cctcacacct
    20461 cacacctcac acctcacacc tcacacctca caacagcggg tctggacaga aagcggctct
    20521 tgatgtggat tatcgcatgg tgtgggcttt cagcggcatg gtgactggaa agtaacgata
    20581 ttgcatctgt ggcaacgaaa attgcagagt tggtactctt tgaactgcat aagtaagcag
    20641 aaaggtaagg ttatgctttg cgattgaaat tcagccttaa cgtacaataa ttttcgatat
    20701 ccaaactgcc cccctataac agcaaaaggt cccacaagac gctcgtggaa tatattttaa
    20761 aagagaacaa aaattgcaat atgtggtggc tccatacaga ggattgacaa cattcccatg
    20821 ctaattgtta agattcaaat tgaagtatat ctatctgtca tttttgcaaa tgacatgcgg
    20881 tagcataacg ttccgggggc tcctgtgccg tcatgcgtga cgacattgat cagccaataa
    20941 tggcacgtga ccacagccag atacggccag ccttcatccg tacgtaaata tgtgatgtct
    21001 cccgcccact tctggttcgg gccactggcg taaaaatcct gctccagcag attttctgac
    21061 acaggctggc tgtgtgcgcg gtaacagacc ggactgaaat tccaggaagc ttttgccctc
    21121 agtccgtgac ggcgcaggct ttccgtcacg gtttttactt taaaatggta accctgggca
    21181 cgcagttcat ccatcaggcg ttgtgcatga ccgggtaaaa gcaacgagga caacgctgtc
    21241 gcagtgctga cggaactgcc gacgtgtact tatccttatc cgccagtgat ataagacggt
    21301 aattcaccat ttggattgtc cgctccaccc aacatgttgt ttccttgagg ttctcacacc
    21361 agaaaggaca tcagcatgct gagcagagag gacttttaca tgataaagca aatgcgccag
    21421 caaggtgcgt atattgttga tattgcgact cagattggtt gctctgaacg aacggtcaga
    21481 cgttacctga aatacccaga acctccggcc agaaaaacac gccacaaaat ggccgaaatg
    21541 gactatatcg acatgcgtct ggctgagaat gtctggaacg gcgaagttat cctggcggaa
    21601 atcaaagcga tgggttatac cggtggccgt tccatgttgc ttcgccagtt caggaagaca
    21661 ccggagcagc gctttacgca ggaacacagt ctgttcttcc ggttgctgaa ccgtcgatat
    21721 gaaaaagcga gcattgtgct gacgtccaat aaagggttcg ccgactgggg gagagatgtt
    21781 cggtgataac gtgctggcaa aatattcacc caacgcattg agcgtaataa tctgacgcta
    21841 cgcacccgca ttaagcggtt gactcgtaaa accatctgct tctcacgctc agttgagatc
    21901 cacgaaaaag tgatcggagc gtttatcgaa aaacacatgt tctactaatt ggatgcacca
    21961 ccgaatacat taatttatat aacaacatca aaatacattt taggttgtcg ctaatcaaaa
    22021 attattaagt gttacataat tagagggtat agatatgatt catcctggct cttctttaga
    22081 taaagcaatt aacaatacaa gagttaaaaa tgttagtact gatgttaaac atggccaaat
    22141 tcaagagaga aaaaggaact ttatctataa aaaaaatgat gatatctcat ccagatttaa
    22201 actttacagt tcgctcgtaa agcagaaaaa tgcgacagaa gatgttgttt taataggcaa
    22261 aatgatttta gatgaagtta gaagttacag aactatacat aatgatcgaa atatcgtaag
    22321 taactcagga aactggaaaa catctttttt atgtaatctt gctagactac tatatagcat
    22381 atttaatggt agtaactatt tttgttcccg agagggtgaa aataattcat cccccagttc
    22441 tactctactt actatacatc agcctgaaaa gcaggaacta ttacaacaaa agagtatcaa
    22501 acatttacca acaagtaata acatcgacgg atacattaaa ataagaaaaa caagaggcgc
    22561 tgaagatcaa acaacaacta tcactcaaag tttgataatt aatgagttgt taaatggagt
    22621 tgatagaaat accatccctt ttcagaaaat aagtgagctc aatgatatca tacattcata
    22681 tgaaaatatg caaattaaaa atagtcgaaa aggtatagaa atacttgtta agcagggaga
    22741 gctgttatca tcattaataa atgataataa aggaaataaa caattatcag acaatgcatc
    22801 taaaataata aacttattgg gtatagagta tcagtcacat aaagtagaca tagagccatt
    22861 tatacatgca gtatgggttg ctggcgcacc tcctgataat acatttt
//
LOCUS       DAZFBR010000079        22258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFBR010000079 DAZFBR010000000
VERSION     DAZFBR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.079"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            38..172
                     /locus_tag="ACHJZ2_003275"
     CDS             38..172
                     /locus_tag="ACHJZ2_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO4569335.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            151..255
                     /gene="speFL"
                     /locus_tag="ACHJZ2_003276"
     CDS             151..255
                     /gene="speFL"
                     /locus_tag="ACHJZ2_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO4569336.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            566..2764
                     /gene="speF"
                     /locus_tag="ACHJZ2_003277"
     CDS             566..2764
                     /gene="speF"
                     /locus_tag="ACHJZ2_003277"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO4569337.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2761..4080
                     /gene="potE"
                     /locus_tag="ACHJZ2_003278"
     CDS             2761..4080
                     /gene="potE"
                     /locus_tag="ACHJZ2_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO4569338.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4137..5777)
                     /gene="pgm"
                     /locus_tag="ACHJZ2_003279"
     CDS             complement(4137..5777)
                     /gene="pgm"
                     /locus_tag="ACHJZ2_003279"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO4569339.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5803..6348)
                     /gene="seqA"
                     /locus_tag="ACHJZ2_003280"
     CDS             complement(5803..6348)
                     /gene="seqA"
                     /locus_tag="ACHJZ2_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO4569340.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6533..7297
                     /gene="ybfF"
                     /locus_tag="ACHJZ2_003281"
     CDS             6533..7297
                     /gene="ybfF"
                     /locus_tag="ACHJZ2_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4569341.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDWEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7437..7730
                     /gene="ybfE"
                     /locus_tag="ACHJZ2_003282"
     CDS             7437..7730
                     /gene="ybfE"
                     /locus_tag="ACHJZ2_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO4569342.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7870..8400
                     /gene="fldA"
                     /locus_tag="ACHJZ2_003283"
     CDS             7870..8400
                     /gene="fldA"
                     /locus_tag="ACHJZ2_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO4569343.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8610..8696
                     /gene="uof"
                     /locus_tag="ACHJZ2_003284"
     CDS             8610..8696
                     /gene="uof"
                     /locus_tag="ACHJZ2_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO4569344.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8689..9135
                     /gene="fur"
                     /locus_tag="ACHJZ2_003285"
     CDS             8689..9135
                     /gene="fur"
                     /locus_tag="ACHJZ2_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO4569345.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9219..9545)
                     /gene="chiQ"
                     /locus_tag="ACHJZ2_003286"
     CDS             complement(9219..9545)
                     /gene="chiQ"
                     /locus_tag="ACHJZ2_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO4569346.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9595..11000)
                     /gene="chiP"
                     /locus_tag="ACHJZ2_003287"
                     /pseudo
     CDS             complement(9595..11000)
                     /gene="chiP"
                     /locus_tag="ACHJZ2_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(11579..13243)
                     /gene="glnS"
                     /locus_tag="ACHJZ2_003288"
     CDS             complement(11579..13243)
                     /gene="glnS"
                     /locus_tag="ACHJZ2_003288"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO4569347.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13446..15392)
                     /gene="nagE"
                     /locus_tag="ACHJZ2_003289"
     CDS             complement(13446..15392)
                     /gene="nagE"
                     /locus_tag="ACHJZ2_003289"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO4569348.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15725..16525
                     /gene="nagB"
                     /locus_tag="ACHJZ2_003290"
     CDS             15725..16525
                     /gene="nagB"
                     /locus_tag="ACHJZ2_003290"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO4569349.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16585..17733
                     /gene="nagA"
                     /locus_tag="ACHJZ2_003291"
     CDS             16585..17733
                     /gene="nagA"
                     /locus_tag="ACHJZ2_003291"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO4569350.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17742..18962
                     /gene="nagC"
                     /locus_tag="ACHJZ2_003292"
                     /gene_synonym="nagR"
     CDS             17742..18962
                     /gene="nagC"
                     /locus_tag="ACHJZ2_003292"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO4569351.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            19010..19762
                     /gene="nagD"
                     /locus_tag="ACHJZ2_003293"
     CDS             19010..19762
                     /gene="nagD"
                     /locus_tag="ACHJZ2_003293"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO4569352.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            20019..21683
                     /gene="asnB"
                     /locus_tag="ACHJZ2_003294"
     CDS             20019..21683
                     /gene="asnB"
                     /locus_tag="ACHJZ2_003294"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO4569353.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            22063..22139
                     /locus_tag="ACHJZ2_003295"
     tRNA            22063..22139
                     /locus_tag="ACHJZ2_003295"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22097..22099,aa:Met,seq:cat)
     gene            22150..22234
                     /locus_tag="ACHJZ2_003296"
     tRNA            22150..22234
                     /locus_tag="ACHJZ2_003296"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22184..22186,aa:Leu,seq:tag)
BASE COUNT         5491 a         5613 c         5580 g         5574 t
ORIGIN      
        1 aacaaaacag catctagtat ggcgctattc ataattaatg atttattatc tggggtatga
       61 tcgttttttg ttgatcttct tcacagatta tagccatttc atggatagaa taactctacc
      121 ttcaactgac acagcaagag gtaaaggtaa atggaaaata ataaccgctt aatgcctcat
      181 ataaggcgga caacccatat catgaagttt gcccatcgta atagttttga ctttcatttc
      241 tttaatgccc gctagtcttc tgactaaagg gcaccccagc gtacaggtct ccctgacttt
      301 aagcattaca ggttaatacc tgtatccctc ggtgctcata tactgctaac ccttttaaac
      361 tctacataat tcgagtcgca gcatatgcaa cttgaagtat gacgagtata gccagttacc
      421 gggctggtct gggttattgc atctgcaaaa agcaaactac tgatttattt atcagcggtg
      481 gagctttgct ttttttccgg cgtgatcgat ttctcctttg agaaattgag gacctgctat
      541 tacctgaaat aaagagatga acaaaatgtc agaattaaaa attgccgtta gtcgttcttg
      601 cccggattgt ttttccactc atcgtgcatg cgtgaatata gacgaaagta attatattga
      661 cgttgccgcc attattttat cagtcagtga tgttgaacgt ggaaaactcg atgaaataga
      721 cgctactggc tatgacattc ctgtttttat tgcaacggaa aatgaagaac gtataccagc
      781 agaatatctt tcacgtattt ctggtgtctt cgaacatgga gaagcacgca aagaatttta
      841 tggtcgtcag ttagaaaccg ctgccagcca ttatgaaact caactgcgcc cacctttctt
      901 tcgcgcactg gtcgattatg tcaatcaggg caacagcgca tttgactgcc ccggtcatca
      961 gggcggcgaa tttttccgtc gccatccggc ggggaatcag tttgtggaat actttggtga
     1021 ggcgctgttc cgtgccgact tgtgcaacgc cgacgtagcg atgggcgatc tgctgattca
     1081 cgaaggcgcg ccatgcattg cacagcaaca tgcagcaaaa gtgtttaatg ccgataaaac
     1141 ctacttcgtt ttaaatggca cttcatcttc taacaaagtg gttttaaacg ccctgctgac
     1201 accgggtgat ctggtgctat ttgaccgcaa taaccataag tctaaccacc acggggcctt
     1261 gctacaggct ggtgcaacac cggtttatct ggaaacagca cgtaacccgt atggttttat
     1321 cggtggtatt gatgcacact gcttcgaaga aagttatctg cgtgaactga ttacagaagt
     1381 tgcaccgcaa cgggcaaaag aggcgcgtcc gttccgcctc gctgtgattc agctaggcac
     1441 ctacgacggt acgatttata acgcccgcca ggtggtggat aaaattggtc atctgtgtga
     1501 ctacatcctg tttgattccg catgggtcgg ctatgaacag tttattccga tgatggctga
     1561 ctgttcgccg ctgttgctgg atcttaatga gaacgatccg ggtattctgg ttacgcaatc
     1621 cgtacataaa cagcaggctg gtttttctca aacttcacaa atccataaaa aagacagcca
     1681 catcaaaggg caacagcgtt atgtaccgca caaacgcatg aacaacgcct ttatgatgca
     1741 tgcctccacc agcccgttct atccgctgtt tgccgcactg gatatcaacg ccaaaatgca
     1801 tgaaggtgtc agcggtcgta atatgtggat ggattgtgtg gtaaatggca ttaatgcccg
     1861 caaactgatc ctcgataact gtcagcatat tcgtccgttc atacctgaac tggtggatgg
     1921 taaaccctgg cagtcgtatg aaacagcgca aattgcggtt gatctgcgct tcttccagtt
     1981 tgtaccaggt gagcactggc attcttttga aggctatgca gagaatcaat actttgttga
     2041 tccgtgcaaa ctgttgctga caaccccagg tattgatgca cgtaacggcg aatatgaagc
     2101 gttcggtgta cccgcgacga ttcttgctaa cttcctgcgc gaaaatggcg tagtgccgga
     2161 aaaatgcgat cttaactcca tcctcttcct gctgactccg gcagaagata tggccaaact
     2221 tcagcaactt gttgccctgc tggtacgctt cgaaaaactg ctggaatctg atgcgccgct
     2281 ggcggaagtg ctaccttcca tctacaaaca gcatgaagag cgctacgccg gttataccct
     2341 tcgtcagttg tgccaggaaa tgcatgattt gtatgcccgc cacaacgtga aacaactgca
     2401 aaaagagatg ttccgtaagg aacacttccc acgcgtcagc atgaatccgc aagaagctaa
     2461 ctacgcctat ttacgcggtg aagtggagct ggttcgtctg ccggatgcag aaggccgtat
     2521 cgctgccgaa ggtgcgcttc cttatcctcc gggtgtgctg tgtgttgttc cgggtgaaat
     2581 ctggggtggt gctgttttgc gttacttcag cgctctggaa gaagggatca acctgctgcc
     2641 aggttttgca ccggagctgc agggtgtcta tatcgaagaa catgatggtc gtaagcaagt
     2701 ttggtgctat gtcatcaagc ctcgtgatgc gcaaagcacc ctgctgaaag gggaaaaatt
     2761 atgagtcagg ctaaatcgaa caaaatgggc gtcgttcagt taaccatact gacgatggtc
     2821 aacatgatgg gctccggtat catcatgctg ccgacaaagc ttgccgaagt tgggacaatc
     2881 tcaattatct cctggctggt gacagccgta ggctcaatgg cactggcatg ggcattcgca
     2941 aaatgcggta tgttcagccg taaatcaggc ggtatgggcg gttatgccga gtatgctttc
     3001 ggtaaatccg gtaactttat ggcgaactat acctacggcg tctcactgct gattgctaac
     3061 gtcgcgattg ctatttcggc ggttggttac ggcaccgaac tgctcggcgc gagtttgtcg
     3121 ccagtgcaga ttggtcttgc gaccatcggg gtgctgtgga tttgtaccgt ggctaacttt
     3181 ggtggtgcgc gcattaccgg gcaaatcagt agcattactg tgtggggagt cattattccg
     3241 gtcgtcggtc tgtgcattat tggctggttc tggtttagcc cgacgctgta cgttgattcc
     3301 tggaatccgc atcatgcacc gttcttcagt gcggtaggtt cttccatcgc tatgacgctg
     3361 tgggcttttc ttggtctgga gtctgcgtgt gcaaatactg atgtagtgga aaacccggaa
     3421 cgtaatgtgc caatcgcggt actcggcggt acgttaggtg cggcggtgat ttatatcgtc
     3481 tccaccaacg tgattgccgg gattgtgcca aatatggagc tggcaaattc aacggcacca
     3541 tttggtctgg ccttcgcgca gatgttcacg ccggaagtgg gtaaagtcat tatggcgctg
     3601 atggtgatgt cctgctgcgg ttcgctgctt ggctggcagt tcaccattgc ccaggtgttt
     3661 aaatcttcat ctgatgaagg ctacttccct aaaattttct cccatgtaac caaagtggat
     3721 gcaccggtgc agggaatgtt gaccattgtg attatccaga gcgggttggc gctgatgacg
     3781 attagcccgt cgctaaacag tcagttcaac gtgctggtta acctggccgt ggtgaccaat
     3841 atcattccgt atattctgtc aatggcggca ttagtcatta ttcagaaggt cgctaatgtg
     3901 cccccctcaa aagcgaaagt tgcaaacttt gttgcttttg ttggcgcgat gtacagcttt
     3961 tatgcgctgt actcatccgg ggaagaagcc atgctgtacg gttccatcgt gaccttcctt
     4021 ggttggacac tgtatggtct ggtatcacca cgctttgaac tgaaaaataa acacggttaa
     4081 aaaagcaaag taaaaaaggg cgatcttgcg accgcccttt ttcttattta atgtgtttac
     4141 gcgtttttca gaacttcgct aacaatctca accgcttctt tctcaatctg cttgcgatgt
     4201 tcttcaccga ggaagctttc gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc
     4261 gcggcgaacc agccgttgtc agtcatcact ttcagaccgc caatagaagc accgttgccc
     4321 ggagcagcag tcaggcgcgc ggtgatcggg tcaccagcca gggtgctggc actcaccatt
     4381 tccggagaca gcttagacag cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg
     4441 ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt agtgttcctg cgggttctta
     4501 ccggtgacag cggtgatttc cgccgccagc agacacatga tgatgccgtc tttgtcggtg
     4561 gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg cgctctcttc gccgccaaag
     4621 ccgaagctgc cgtcgaacag accatcaaca aaccatttga aacctaccgg gacttctacc
     4681 agtttacggc ccaagtcgtt gaccacacgg tcgatcatcg cagatgaaac cagcgtttta
     4741 ccgacggcaa cagctttgcc ccactgcgga cgatgctgga acaggtagtt gattgccacc
     4801 gccaggtagt ggttcggatt catcaaacct gccggagtga cgataccgtg acggtcataa
     4861 tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca gtgccagcag gcccgccatc
     4921 gcacactcgg aggagcagtc catacggatc gcgccgtctt tatcaaggtg cataaagcgg
     4981 aaggtttgat cgacctgatc gttaacgatg gtcaggttga ggttgtaata ctcgccaata
     5041 cgcttccagt attcgatacc ggaaccgccc agcggatcaa cgccaagcgt caggcccgct
     5101 ttctgaatcg ccgccatatc aacgatatcg gccagccctt ccacaaacgg ctgcaccaga
     5161 tcctgctctt tcacatgacc ggatgccatc gcttcgtcga gggagatacg cttcacgcct
     5221 ttcaggccat cggccatcag tgcgttggct ctgtcttcca ccactttagt gacgttggta
     5281 tcagccgggc caccatttgg cggattgtac ttgataccac catcttccgg cgggttatgg
     5341 gacggtgtaa taacgatacc gtctgccagc gggccacctt ttttattgtg aaccaggatg
     5401 gcattggaaa cggcaggcgt cggggtgaag ccattgtttt cctgcacaat aacatctacg
     5461 ccgttcgctg ccagcacttc cagaacggaa ataaatgcag gttcggacag ggcgtgagta
     5521 tctttaccca catagcaagg gccagtgatg ccgtttttcg cacgttcttc agcaattgcc
     5581 tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc gcgctgcact gccacggtga
     5641 ccggaagtac cgaatttcac cgcgtgctcc gcattccctg cttctggttt cagtacataa
     5701 tattgcgccg tcagttgggc gacgttaatc aaatcactct gttgtgcagg ttggcctgca
     5761 cgattgtgga ttgccattgc tttgtccttt gtctgcaacg ttttagatag ttccgcaaac
     5821 cttctcaatc aattccgccg ggaattgcat cgactgcatg atgtgttcga tcatgctgca
     5881 tttacggccg gtgttggtgt tggtgatcac ccaatacggc gtgcctggca catgtttcgg
     5941 cttggtctga ttaccatttt tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt
     6001 acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt
     6061 agacaacagc agcataaagc gattgaccgc tcgcttttgc tctgcatatt catccgaaag
     6121 cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt ttgatcggct tcgcttcgac
     6181 gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc
     6241 ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg
     6301 gctggcaata tagctgtaga gttcatcatc aacttcaatc gttttcatct taatccagtg
     6361 cagtgtctta gctgaataca agtcgccagg agtatatgaa taaatcccgg cagcggatag
     6421 cgtcaagcct ggcttgcaga aaaactcgga ataaacctta tttgttgcag ccctgtggca
     6481 caatggtcaa cataataccc taacccgaca acgcgaaaaa aagaactttg ccatgaaatt
     6541 gaatatccgc gcgcaaactg cacaaaacca gcacaataat tctcccatcg ttcttgtcca
     6601 tggtctgttt ggcagcctcg acaaccttgg cgtactggct cgcgatctgg taaacgatca
     6661 caatatcatc caggttgata tgcgtaacca cggtctttca ccgagagatc cggtaatgaa
     6721 ttacccggcg atggcgcagg atcttgttga tactctggat gcacagcaga tcgacaaagc
     6781 aacatttatc gctcactcca tgggcggtaa agcggtaatg gcacttactg cactggcccc
     6841 cgatcgcatc gataaactgg tggcgatcga tatcgcaccg gtcgactatc acgtacgccg
     6901 tcatgatgag attttctcgg ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca
     6961 gcaagcagca gcaataatgc gccagcatct taatgaagaa ggggtgattc agtttctgtt
     7021 gaaatctttt gttgactggg agtggcgctt taacgtgccg gtattgtggg atcagtatcc
     7081 gcatattgtg ggttgggaga aaatcccggc atgggatcac cctgccctgt ttatccctgg
     7141 tggtaattct ccgtatgtta gcgagcagta ccgtgatgat ttactggcac aatttccaca
     7201 ggcacgggcg catgtgattg caggcgcggg tcactgggtc catgctgaaa aaccggatgc
     7261 ggtattacgc gctatccgtc gctatctcaa tgattaacgc aactgattaa aaacccagcg
     7321 tccgcgtcgc tttccgcgcg tgggcgttgg cgtgactcat cgcgctgatg tattatggcg
     7381 cgctatccat ccgggcagag gcctggctga ttgtttcccc cgaagtcacc aagatcatgg
     7441 ccaaagaaca aacggaccgt acgacattag atctgttcgc gcacgagcgt cgaccgggac
     7501 gaccgaaaac aaatccgctt tcgcgcgatg aacagctgcg tattaataaa cgcaaccaac
     7561 taaaacgcga caaagtacgt ggcctaaagc gtgtcgaact gaagctgaac gcggaagctg
     7621 tcgaggcgct gaacgagctg gcggaatcgc gcaatatgag ccgtagcgaa ctgatcgaag
     7681 agatgttaat gcagcaactg gcggcgctgc gtagtcaggg catcgtttaa atttccactt
     7741 tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc cgcaagactg cctgttctgc
     7801 tatgattgcc tttatccgtg ggcaattttc cacccccatt tcaataagtt tcaagaggtt
     7861 atttcactca tggctatcac tggcatcttt ttcggcagcg acaccggtaa taccgaaaat
     7921 atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg ccgatgtcca tgacattgca
     7981 aaaagcagca aagaagatct ggaagcttat gacattctgc tgctgggcat cccaacctgg
     8041 tattacggcg aagcgcagtg tgactgggat gacttcttcc cgactctcga agagattgat
     8101 ttcaatggca aactggttgc gctgtttggt tgtggtgacc aggaagatta cgccgaatat
     8161 ttctgcgacg cattgggcac catccgcgac atcattgaac cgcgcggtgc aaccatcgtt
     8221 ggtcactggc caactgcggg ctatcatttc gaagcatcaa aaggtctggc agatgacgac
     8281 cactttgtcg gtctggctat cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta
     8341 gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg acgaaattct caatgcctga
     8401 tgtgatgcgg cgtagactca tgtctacgcc gtattaatag ataatgccaa tcaaaataat
     8461 tgctacaaat ttgtaacttt tgccgttgta cctgtacaat gtcccggtgt tcaagtggcc
     8521 ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac aatatttgcc agggacttgt
     8581 ggttttcatt taggcgtggc aattctataa tgatacgcat tatctcaaga gcaaattctg
     8641 tcacttcttc taatgaagtg aaccgcttag taacaggaca gattccgcat gactgataac
     8701 aataccgccc taaagaaagc tggcctgaaa gtaacgcttc ctcgtttaaa aatcctggaa
     8761 gttcttcagg agccggacaa ccatcacgtc agtgcggaag atttatacaa acgtctgatc
     8821 gatatgggtg aagaaattgg tctggctacg gtatatcgcg tactgaacca gtttgacgac
     8881 gctggtatcg tcacccgcca caattttgaa ggcggtaaat ccgtatttga actgacacag
     8941 caacatcacc acgatcacct gatctgcctc gactgcggca aggttatcga atttagtgat
     9001 gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac atggcattcg cctgactaac
     9061 cacagtctct atctttacgg tcactgcgcc gaaggcgatt gccgcgaaga tgagcacgcg
     9121 cacgaaggca aataagccag tctgaacgag aaaagccaac ctgcgggttg gcttttttat
     9181 gcaagggaaa taagaaaagc gagagttaca gctctcactt atttgttatt actgcgaatt
     9241 tcctgccaga ccttatcaca atcggctttc accgcctgat catttccggt ttgcgttgca
     9301 cgcagacact gctgataatc cagtacgcgg acactttcct ggtcggcaaa ctgctgatgt
     9361 tgtttctctt tcttcagcac gtttaacacg ctctggcagg cttcaatttt ttccggcgaa
     9421 ccttgtgcgg tgttgataca ggcgctgtat gcctctttca gacggctgtc ttcctgtggc
     9481 gcggttgatt gcgcacaagc taccagcccc gatgccatca tggcgatgag aatcagtttt
     9541 ttcatagcaa attcctcaaa cgtgcggcag gcacaaaacc tgccgcgtcg ggcatcagaa
     9601 gatggtgaat ggtgcgatta ccataaattt tacgtcacgc tcatcctgga agatgttgcc
     9661 gtaaccgccg ccccagcttg ggatgtcgga gtggttgtcg tattcggtga agtgcagttt
     9721 gaacatcgtg cctttggcgc gaccgtcctg aatggtgtag accgcatcca ggctgtatgc
     9781 agactcttca atagtccggt ttttgtcgta gtacgcatcc ggattgctct gccaggtcgc
     9841 aggtttagca tcccatgcgt aaacgtagga agcgccgatg gcgaagcctg gaagattcca
     9901 gtttttcagg tcatacatcg caccgaagaa gaccgctttt tcgccgttgg cgttgaagtc
     9961 agaacggtta tcccaccaga tatccaggcg accgttcgag gaagcgtagg ttggagtcat
    10021 acgttgcagg aagtatccct gctgaccgtc agctttaacc caggtgcctt cgaggcgcaa
    10081 atccactacg tcagccgccc ggtaaccaaa ggtcaacgct tgcagccagg cggtgccgtc
    10141 ataaagatcg ttgacgctgc gatcgtcaac tttatcgcgc gtaccgtaga actggtagct
    10201 ggtggttaac gggctaccgg cgatatcaaa tttgtagctg gctttggcaa agtattgatc
    10261 gatataccct tccgcctgac caaatgccgc ttccagtacg aagttatttt tgaagtcgta
    10321 tttcgcccca agggagtgca gataatcaac tttggtggtt ttatcgttct gataaaactc
    10381 atccatttcc agatgccacg gtgctttgta ttcgttggtc cacatgtagg agaaactcaa
    10441 cgcaccagca tcgccgtaat caaaattcgc cccggcctcc gcaccctgat aagtacccgg
    10501 cataaagctc cagtgcggcg ctaacagcgt ttgaccagtt ggctgaatat aacctgccct
    10561 cgccacaccg gaccgtattt aaatttggcc gctgctttat acaggcttat accgctttta
    10621 tcgccggacc agtcttcgtc ataggcttta ttactttttg aaaacgcgat ttcgttcggg
    10681 tgagagctgt cgccgttttc cgccatttca atcgccgtaa acgcggcaat atcaaggccg
    10741 aacatatcag cagcataacc ggactgaaaa tcgaggttgg cgttccaggt ggagtgagaa
    10801 aggttggttt tgtatttatc gccgtcggta acatctttac ggtcgcgttc acgctgccag
    10861 taatagatac cgccggttaa ggttgaatcg tcaatgaatc cttcggcgcg agcctccggc
    10921 attgccataa agcccgacat tgctgtaaca ccggcgatag ccagcgccag cgtactacgt
    10981 ttgccactaa acgtacgcat gggttaatcc tctttgacgt ataaattgct gcaccacagg
    11041 tgaacagcga aaaaaataaa aattaaaaaa gtagcgttga gaaaagtctc gctgactaca
    11101 ggaacagact atttttcgcg acgcgaatta tcaatctctt tctgtaacca gaatgtaaga
    11161 acatgagcaa ccgcacatat tttgatgatt tttgttacaa ggcttaagtt tgcggttgaa
    11221 aggcttgcag aagaataaaa gcgtgaaatt ccagcaagat gaaagcggtt acattttatc
    11281 taattatgtt ttgtgttttt aaatttattt tatggataaa ttgcgaagcg gaattaattc
    11341 gcgaagaaaa gcagaaaaaa cgccgctgaa cgcggcgttt tggattgttt gcatatttct
    11401 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag cacgcaaact caatatattg
    11461 cagagatcat gtaggcctga taagcgtagc gcatcaggca atttagcgtt tgaaactgtt
    11521 tgtctgttcc ttgccggatg cgacgtaaac gccccatccg gcatagcgaa acttaaaatt
    11581 actcgcctac tttcgcccag gtatcacgca gcccaacggt gcggttaaat accggttttt
    11641 ccgccgtaga atggcggcta tcgaggcaga agtaaccttc acgctcaaac tggaatgctt
    11701 tacccgcaac cgcatctttc agcgacggtt cagcaaagcc ctgtttgatc accagcgatt
    11761 ccgggttaat caccgacagg aaatcatccg cagcacctgg gttcggcacg ctgaacagac
    11821 ggtcatacaa acggatttca accggcagcg catgtgccgc gctcacccag tgaataacac
    11881 ctttgacttt acgaccatct gccggatctt tgcttaaggt atcggcgtca taagtacaga
    11941 agatggtggt gatattacct tcggcatctt tctcgacgcg ttctgcctta atcacataag
    12001 cattacgcag acgcacttct ttacccagca ccagacgttt gtactgcttg ttagcttctt
    12061 cgcggaaatc ggagcgatca atccaaatct caccgctaaa cggcacctga cggctcccca
    12121 tttccggttt gttcggatgg ttcggcatgg taaccatttc gccttcgccc tgatagtttt
    12181 cgataaccag tttcaccgga tcgataaccg ccattgcgcg cggcgcattt tcgttgagat
    12241 cttcacggat gcaggattcc agcgacgcca tctcaatggt gttgtcctgc ttggtcacgc
    12301 cgatgcgttt gcagaactca cgaatagaag ccgcagtgta accacgacga cgcagaccgg
    12361 aaatggtcgg catacgcggg tcatcccagc cttcaacgtg cttgtcggtc accagcaagt
    12421 tcagcttacg cttggacatc acagtgtatt ccagattcag gcgcgagaat tcgtactggc
    12481 gcgggtgaac aggaatggtg atgttgtcca gtacccagtc gtacagacga cggttatcct
    12541 ggaactcaag cgtacacaga gagtgcgtaa taccttccag ggcatcgctg atgcagtggg
    12601 tgaagtcgta catcgggtag atgcaccact tgttgccagt ctggtggtgt tcagcaaatt
    12661 taatgcggta cagcaccgga tcgcgcatca cgataaacgg tgaagccatg tcgattttcg
    12721 cacgcaggca ggctttacct tcttcaaaac cgccggtacg cattttctcg aacagcgcca
    12781 ggttctcttc aacgctacgg tcgcggtacg ggctgttttt accaggctgc gtcagggtgc
    12841 cgcggtattc gcggatctgt tccggcgtca gttcatcaac gtacgccagg cctttattga
    12901 tcagttcgat cgcataggcg tggagctgat caaaataatc ggaggagtaa cggacgttac
    12961 cagaccagtg aaaacctaac cactcaacgt cgtttttgat cgaatcgaca tactcgatat
    13021 cttcttttac cgggttagtg tcgtcgaaac gcaggttgca ctggccttta tagtcctggg
    13081 cgatcccgaa gttcaggcag atagatttcg catggccaat atgcagatag ccattcggct
    13141 ccggcgggaa acgggtgtgt actgtggtgt gcttaccact ggccagatct tcatcgatga
    13201 tctgacggat aaagttagtc gggcgggctt ctgcctcact catcgtggat tcctcaaagc
    13261 gtaaacaacg tataacggcg tatgatctta taagcgggac aggctgacaa ctgttagtta
    13321 gtgaaaatcg aaaatctcac tgagattatg gggatgattg atgaaaaaaa aagcggcaga
    13381 gaagatctcc accgcttgtc attgttggat gcgacgctca agcgtcgcat caggcataaa
    13441 gcagattact ttttgatttc atacagcggt gtttgacccg ccacaacatg gccctgagct
    13501 ttaatgatca agccactgaa atcgtcgata ttgctgcaaa ccaccgggct aatcatcgaa
    13561 cgggcgttag cgttcaggta atccagatcc atttccagaa tcggttgccc tgcgcttacc
    13621 tgcgcgccct cttccaccag acgtttaaag cctttacctt ccagcgctac ggtatcgata
    13681 cccatgtgaa cgacaatctc cgcgcctttt tcggtttcca ggcagaacgc gtggttggtg
    13741 ttgaagattt tcacgattgt tcctgcggct ggtgatacga cgattttatc tgtcggtttc
    13801 accgccacac cgtcacccac cgctttgctg gcgaatgctt cgtcaggaac ctgatccagt
    13861 gccacgacat caccggtaat cggcgatacc agctccgcga tagataccgc gtttggtaca
    13921 gcctgcggtt ttgctacagg agcggcagtt gccggagttg cttcagcaga ggcagctgct
    13981 accgggccac gggcaacgac tttcttcatc gcatcgccga tggattctgc tttcgcgcca
    14041 acaatcacct gaatagtctg tttgttcagt tttactaccc cagaagcccc cagacgttta
    14101 cacatcgtat cgttaacgcg ggcagagtca gccacggtaa ggcgcagacg ggtgatacag
    14161 gcgtcaattg ctttcaggtt gtcagtgccg ccaaccgcag caatatagtt ggtcgccagt
    14221 tgattgaggc cttcttcagt gttgctgttg gcttcttcag taacgatctc gtcttcttta
    14281 tcttcacgac ccggcgtttt caggttgaac atgcggataa ccaaactgaa caccacgaag
    14341 tagatagcga agaagacaac acccataacc agcagcatcc agacgttctg gctggcggcc
    14401 ggcaggttat acatcaacgc gtagtcgata gcccccgcag agaaagagaa gccggcatgg
    14461 atacccagca gcgttgccac aaacaggctg ataccggtca gcagtgcgtg caggaggtac
    14521 agcagcggag caaggaacat gaacaggaat tccagcggct cagtcacacc ggtcaggaac
    14581 gcagtaacag caacagaaag cagcatcccg ccaaccatcg gacgacgctc tttcggtgct
    14641 gcgaagtaca tcgccagcgc cgcacccggc aggccgaaca tcatgatcgg gaagaagccg
    14701 gacatgaaca tccccgcggt gccgtcacca gcgtagaaac ggttgatgtc gccgtggaaa
    14761 accgtacccg ccgcgttggt gaattcacca atctggaacc aggcgatggt gttcagcacc
    14821 tgatgcagac cggttgggat caacagacgg ttgatgaaac caaagatacc ggaacccagc
    14881 gcgcccgcag aaacgatcca ctcgccgcct gcatggatag cgtgctgtac cggcggccag
    14941 acgtaaccaa aaatggccgc cagcaccaga cagaagaagc cggtggcgat cggcacaaag
    15001 cgtttgccgc cgaagaagct caggaagtcc ggcagtttaa tatcggacca acggttatag
    15061 gctgcgccac caaccagacc ggtaatgata cccgccagta cacccatgtt aatttctggg
    15121 ttgatggtca ccatcgcttt ggttaacaca aagtaaccta ccgcccccgc cagcgccgcc
    15181 gcacctgcgc tgtctttcga ccagctggat gccacaccga tggcgaagat taatgcgagg
    15241 ttatcaaaaa tcgcaccgcc cgcctgggca ataaacgcaa cgttaagtaa atctggctga
    15301 ccgaatcgca gcaacagtgc cgccaccggc agcaccgcga tagggagctg taacgcccta
    15361 ccgagtcgct ggaaaaaacc taaaatattc atcttattcc ccctacgaga accctatttg
    15421 gctcgtttaa agccgtattt ttattgtgct gcaaattgta ctgccgatgt tctgtaatca
    15481 gattgttaga tcatctgcca cagagtgtgt gaaaatttaa ttcgtatcgc aaattaaacg
    15541 agtgtctttt gtgagttttg tcaccaaata tcgttatcat cactcccttt tactggctaa
    15601 accagaaaac ttattttatc attcaaaaaa tcaggtcgga ttgacgcctg tctgcgcaaa
    15661 tccaggttac gcttaaagat gcctaatccg ccaacggctt acattttact tattgaggtg
    15721 aataatgaga ctgatccccc tgactaccgc tgaacaggtc ggcaaatggg ctgctcgcca
    15781 tatcgtcaat cgtatcaatg cgttcaaacc gactgctgat cgtccgtttg ttctgggcct
    15841 gccaactggc ggcacgccaa tgaccaccta taaagcgtta gtcgaaatgc ataaagcagg
    15901 ccaggtcagc tttaagcacg ttgtcacctt caacatggac gaatatgtcg gtctgccgaa
    15961 agagcatccg gaaagctact acagctttat gcaccgtaat ttcttcgatc acgttgatat
    16021 tccagcagaa aacatcaacc ttctcaacgg caacgccccg gatatcgacg ccgagtgccg
    16081 ccagtatgaa gaaaaaatcc gttcttacgg aaaaattcat ctgtttatgg gcggtgtagg
    16141 taacgacggt catattgcat ttaacgaacc ggcgtcttct ctggcttctc gtactcgtat
    16201 caaaaccctg actcatgaca ctcgcgtcgc gaactctcgt ttctttgata acgatgtcaa
    16261 tcaggtgcca aaatatgccc tgactgtcgg tgttggtaca ctgctggatg ccgaagaagt
    16321 gatgattctg gtgctgggta gccagaaagc actggcgctg caggccgcag ttgaaggttg
    16381 cgtgaaccat atgtggacca tcagctgtct gcaactgcat ccgaaagcca tcatggtgtg
    16441 cgatgagcct tccaccatgg agctgaaagt taagacttta agatatttca atgaattaga
    16501 agcagaaaat atcaaaggtc tgtaattgtt atccctgccc tggctccttg ctcagggcaa
    16561 tattttttaa aatcgggggt cagaatgtat gcattaaccc agggccggat ctttaccggc
    16621 cacgaatttc ttgatgacca cgcggttgtt atcgctgatg gcctgattaa aagcgtctgt
    16681 ccggtagcgg aactgccgcc agagatcgaa caacgttcac tgaacggggc cattctctcc
    16741 cccggtttta tcgatgtgca gttaaacggc tgcggcggcg tacagtttaa cgacaccgct
    16801 gaagcggtca gcgtggaaac gctggaaatc atgcagaaag ccaatgagaa atcaggctgt
    16861 actaactatc tgccgacgct tatcaccacc agcgatgagc tgatgaaaca gggcgtgcgc
    16921 gttatgcgcg agtacctggc aaaacatccg aatcaggcgt taggtctgca tctggaaggc
    16981 ccgtggctga atctggtgaa aaaaggtacc cataatccga attttgtgcg taagcctgat
    17041 gccgcgctgg tcgatttcct gtgtgaaaac gccgacgtca ttaccaaagt gaccctggca
    17101 ccggaaatgg ttcctgcgga agtcatcagc aaactggcaa atgccgggat tgtggtttct
    17161 gccggtcact ccaacgcgac gttgaaagaa gcgaaagccg gtttccgcgc ggggattacc
    17221 tttgccaccc atctgtacaa cgcgatgccg tatattaccg gtcgtgaacc gggcctggcg
    17281 ggcgcgatcc tcgatgaagc tgacatttat tgcggtatta tcgctgatgg cctgcatgtt
    17341 gattacgcca acattcgtaa tgctaaacgc cttaaaggcg acaaactgtg tctggttacc
    17401 gacgccaccg cgccagcagg tgccaacatt gaacagttca tttttgcggg taaaacaata
    17461 tactaccgta acggactttg tgtggatgag aacggtacgt taagcggttc atccttaacc
    17521 atgattgaag gcgtgcgtaa tctggtcgaa cattgcggta tcgcactgga tgaagtgctg
    17581 cgtatggcga cgctctatcc ggcgcgtgcg attggcgttg agaaacgtct cggcacactc
    17641 gccgcaggta aagtagccaa cctgaccgca ttcacacctg attttaaaat caccaagacc
    17701 atcgttaacg gtaacgaggt cgtaactcaa taagagaaag tatgacacca ggcggacaag
    17761 ctcagatagg taatgttgat ctcgtaaaac agcttaacag cgcggcggtt tatcgcctga
    17821 ttgaccagta cgggccaatc tcgcggattc agattgccga gcaaagccag cttgcccccg
    17881 ccagcgtaac caaaattacg cgtcagctta tcgaacgcgg gctgatcaaa gaagttgatc
    17941 agcaggcctc caccgggggc cgccgcgcta tctctatcgt caccgaaacc cgcaatttcc
    18001 acgcaatcgg cgtacggctt ggtcgtcacg acgccaccat caccctgttt gatctcagca
    18061 gcaaagtgct ggcagaagaa cattacccgc tgccggaacg tacccagcag acgctggaac
    18121 atgccctgct gaatgccatt gctcagttta ttgatagcta ccagcgcaaa ctgcgcgagc
    18181 tgatcgccat ttccgttatc ctgccaggac ttgttgaccc ggacagcggc aaaattcatt
    18241 acatgccgca tattcaggta gaaaactggg ggctggtaga agcactggaa gagcgtttta
    18301 aagtgacctg tttcgttggt cacgatatcc gtagtctggc gctggcggag cactacttcg
    18361 gtgcaagtca ggattgcgaa gactccattc tggtgcgtgt ccatcgcgga accggggccg
    18421 ggattatctc taacgggcgc atttttattg gccgcaacgg caacgtcggt gaaattggcc
    18481 atattcaggt cgaaccgctg ggtgaacgct gccactgcgg caactttggc tgcctggaaa
    18541 ctatcgctgc caacgccgct attgaacaac gggtgttgaa tctgttaaag cagggctacc
    18601 agagccgcgt gccgctggac gactgcacca tcaaaactat ctgcaaagcc gcgaacaaag
    18661 gcgatagcct ggcctcggaa gtgattgagt atgtcggtcg tcatctgggc aaaaccatcg
    18721 ccattgctat caacttattt aatccgcaaa aaattgttat tgccggtgaa atcaccgaag
    18781 ccgataaagt gctgctccct gctattgaaa gctgcattaa tactcaggcg ctgaaggcgt
    18841 ttcgtactaa tctgccggtg gtacgttctg agctagacca ccgctcggca atcggtgctt
    18901 ttgcgctggt aaaacgcgcc atgctcaacg gtattttgct ccagcatttg ctggaaaatt
    18961 aatgtgcttt tatagtggcg cttattgttg tcaatattct gggtagtcca tgaccattaa
    19021 aaatgtaatt tgcgatatcg acggcgtgct gatgcacgat aacgtcgccg taccgggtgc
    19081 agcggaattt ttgcacggga ttatggataa aggcctgccg ctggtgttgc tgaccaacta
    19141 tccttcgcag accgggcaag atctggcgaa ccgctttgcc actgccggtg tcgatgtacc
    19201 tgacagcgtg ttttatacct ctgcgatggc gaccgccgat ttcctgcgtc gccaggaagg
    19261 caagaaagcg tatgttgtgg gcgaaggcgc actgattcat gaactgtaca aagccggttt
    19321 cactattacc gatgtgaacc ctgattttgt gattgttggc gaaacgcgtt cctacaactg
    19381 ggacatgatg cataaagcgg cctatttcgt cgctaacggt gcacgcttta tcgccaccaa
    19441 cccggacacc cacgggcgcg gtttttatcc ggcctgtggc gcgttgtgtg cagggattga
    19501 gaaaatctcc gggcgcaaac cgttctatgt tggtaagccg agtccgtgga ttatccgcgc
    19561 ggcattaaac aaaatgcagg cacattcgga agaaacggtg attgtcggcg ataacctgcg
    19621 taccgatatt ctggccggct tccaggcagg tctggagacg attctggtgc tttctggtgt
    19681 ttcgtcgctc gacgatatcg acagtatgcc tttccgcccc agctggattt acccgtcagt
    19741 cgctgaaatc gacgttatct gaatagcaac cacgtctcag gacgtggttt cttattttta
    19801 cccatcatta aaatcatccc attcaataaa acccgccaaa aattatcgat ccatcaataa
    19861 atacatcacc gcaccctccc ctttttcaca aatcagcaat caccattacg ttttttattt
    19921 tttcaccgcc aaatcgcttg cgcacagtgc tgctttgcgg cattttttta aacaagcaaa
    19981 cacaacaagc aacaaatacc aggttaacgg agaaggttat gtgttcaatt tttggcgtat
    20041 tcgatatcaa aacagacgca gttgagctgc gtaagaaagc actcgagctg tcacgcctga
    20101 tgcgtcatcg tggcccggac tggtccggta tttatgccag cgataaagcc attctcgctc
    20161 acgaacgtct gtcaattgtt gacgttaacg cgggggcgca acctctctac aaccaacaaa
    20221 aaacccacgt actggcggta aacggtgaaa tctacaatca ccaggcattg cgcgctgaat
    20281 atggcgatcg ttatcagttc cagacagggt ctgactgtga agtgatcctc gcgctgtatc
    20341 aggaaaaagg gccggaattt ctcgacgact tgcagggcat gtttgccttt gccctgtacg
    20401 acagcgaaaa agatgcctac ctgattggtc gcgaccatct ggggatcatc ccactgtata
    20461 tgggctatga cgaacacggt cagctgtatg tggcctcaga aatgaaagcc ctggtgccag
    20521 tttgccgcac gattaaagag ttcccggcgg ggagctattt gtggagtcag gacggcgaaa
    20581 ttcgttctta ttatcatcgc gactggttcg actttgatgc agtgaaagat aacgtgaccg
    20641 acaaaaacga gctgcgtcag gctctggaag attccgttaa aagccatctg atgtctgatg
    20701 tgccttacgg tgtgctgctt tctggtggtc tggattcctc aattatttcc gctatcacca
    20761 agaaatacgc agcccgtcgc gtggaagatc aggaacgctc tgaagcctgg tggccgcagt
    20821 tacactcctt tgctgtaggt ctgccgggtt caccggatct gaaagcagcc caggaagtgg
    20881 caaaccatct gggcactgtg catcatgaaa ttcacttcac tgtacaggaa ggtctggacg
    20941 ccatccgcga cgtgatttac cacatcgaaa cctatgatgt gaccactatt cgcgcttcaa
    21001 caccgatgta tttaatgtcg cgtaagatca aagcgatggg cattaaaatg gtgctgtccg
    21061 gtgaaggttc tgatgaagtg ttcggcggtt atctttactt ccacaaagca ccgaatgcca
    21121 aagaactgca tgaagagacg gtgcgtaaac tgctggccct gcatatgtat gactgcgcgc
    21181 gtgccaacaa agcgatgtca gcctggggcg tggaagcacg cgttccgttc ctcgacaaga
    21241 aattcctcga cgtggcgatg cgcattaacc cgcaggataa aatgtgcggt aacggcaaga
    21301 tggaaaaaca catcctgcgt gaatgttttg agtcatacct gcccgcaagc gtggcctggc
    21361 ggcagaaaga gcagttctcc gatggcgtag gttacagttg gatcgacacc ctgaaagaag
    21421 tggcggcgca gcaggtttct gatcaacagc tggaaactgc ccgcttccgc ttcccgtaca
    21481 acacaccgac ctcaaaagaa gcgtatctgt accgggagat ctttgaagaa ctattcccgc
    21541 ttccgagcgc cgctgagtgc gtgccgggcg gtccttccgt cgcgtgttct tccgctaaag
    21601 cgatcgaatg ggatgaagcg ttcaagaaaa tggacgatcc gtctggtcgc gcggttggtg
    21661 ttcaccagtc ggcgtataag taagaaatgc gaaacagccc cggtgaaatc cggggctgtt
    21721 tcagttattg aggtgcttgt tgattctgat atgcagtaag gaaatgttcc gggtttgcca
    21781 catcactgtt tgacaactca tcactgtagg ccagtatttc atagattaat ttcccaccac
    21841 tattgttaaa ttccgaattt catcctgtat gacaaaagaa tggctgtagg gtgggaaaaa
    21901 acacaaagta aacaataatt gacgaatata gcgccacgct gttcgcaacc taaccaaaca
    21961 gtcactttcg agcaattttc cttgaaaaag aggttgacgc tgcaaggctc tatacgcata
    22021 atgcgccccg caacgccgat aaggtatcgc gaaaaaaaag atggctacgt agctcagttg
    22081 gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccat
    22141 cttttttttg cgggagtggc gaaattggta gacgcaccag atttaggttc tggcgccgca
    22201 aggtgtgcga gttcaagtct cgcctcccgc accattcacc agaaagcgtt gtacggat
//
LOCUS       DAZFBR010000080        21594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFBR010000080 DAZFBR010000000
VERSION     DAZFBR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.080"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..223
                     /gene="ghoS"
                     /locus_tag="ACHJZ2_003297"
     CDS             <1..223
                     /gene="ghoS"
                     /locus_tag="ACHJZ2_003297"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO4569354.1"
                     /translation="RLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDL
                     IRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            251..424
                     /gene="ghoT"
                     /locus_tag="ACHJZ2_003298"
     CDS             251..424
                     /gene="ghoT"
                     /locus_tag="ACHJZ2_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO4569355.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(543..2060)
                     /gene="lysS"
                     /locus_tag="ACHJZ2_003299"
     CDS             complement(543..2060)
                     /gene="lysS"
                     /locus_tag="ACHJZ2_003299"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4569356.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(2297..3754)
                     /gene="dtpC"
                     /locus_tag="ACHJZ2_003300"
     CDS             complement(2297..3754)
                     /gene="dtpC"
                     /locus_tag="ACHJZ2_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO4569357.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            complement(3813..5960)
                     /gene="cadA"
                     /locus_tag="ACHJZ2_003301"
     CDS             complement(3813..5960)
                     /gene="cadA"
                     /locus_tag="ACHJZ2_003301"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO4569358.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(6040..7374)
                     /gene="cadB"
                     /locus_tag="ACHJZ2_003302"
     CDS             complement(6040..7374)
                     /gene="cadB"
                     /locus_tag="ACHJZ2_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO4569359.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(7740..9278)
                     /gene="cadC"
                     /locus_tag="ACHJZ2_003303"
     CDS             complement(7740..9278)
                     /gene="cadC"
                     /locus_tag="ACHJZ2_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO4569360.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(9538..9697)
                     /locus_tag="ACHJZ2_003304"
                     /pseudo
     CDS             complement(9538..9697)
                     /locus_tag="ACHJZ2_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(9895..9970)
                     /locus_tag="ACHJZ2_003305"
     tRNA            complement(9895..9970)
                     /locus_tag="ACHJZ2_003305"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9935..9937),aa:Phe,seq:gaa)
     gene            complement(10077..10652)
                     /gene="yjdC"
                     /locus_tag="ACHJZ2_003306"
     CDS             complement(10077..10652)
                     /gene="yjdC"
                     /locus_tag="ACHJZ2_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4569361.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(10689..12386)
                     /gene="dsbD"
                     /locus_tag="ACHJZ2_003307"
     CDS             complement(10689..12386)
                     /gene="dsbD"
                     /locus_tag="ACHJZ2_003307"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO4569362.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(12362..12700)
                     /gene="cutA"
                     /locus_tag="ACHJZ2_003308"
     CDS             complement(12362..12700)
                     /gene="cutA"
                     /locus_tag="ACHJZ2_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO4569363.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(12816..14117)
                     /gene="dcuA"
                     /locus_tag="ACHJZ2_003309"
     CDS             complement(12816..14117)
                     /gene="dcuA"
                     /locus_tag="ACHJZ2_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO4569364.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(14235..15671)
                     /gene="aspA"
                     /locus_tag="ACHJZ2_003310"
     CDS             complement(14235..15671)
                     /gene="aspA"
                     /locus_tag="ACHJZ2_003310"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO4569365.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            16008..16484
                     /gene="fxsA"
                     /locus_tag="ACHJZ2_003311"
     CDS             16008..16484
                     /gene="fxsA"
                     /locus_tag="ACHJZ2_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO4569366.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(16500..17756)
                     /gene="yjeH"
                     /locus_tag="ACHJZ2_003312"
     CDS             complement(16500..17756)
                     /gene="yjeH"
                     /locus_tag="ACHJZ2_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO4569367.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPILIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            18032..18325
                     /gene="groS"
                     /locus_tag="ACHJZ2_003313"
     CDS             18032..18325
                     /gene="groS"
                     /locus_tag="ACHJZ2_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO4569368.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            18369..20015
                     /gene="groL"
                     /locus_tag="ACHJZ2_003314"
     CDS             18369..20015
                     /gene="groL"
                     /locus_tag="ACHJZ2_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO4569369.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            20153..20506
                     /gene="yjeI"
                     /locus_tag="ACHJZ2_003315"
     CDS             20153..20506
                     /gene="yjeI"
                     /locus_tag="ACHJZ2_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO4569370.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(20699..21568)
                     /gene="yjeJ"
                     /locus_tag="ACHJZ2_003316"
     CDS             complement(20699..21568)
                     /gene="yjeJ"
                     /locus_tag="ACHJZ2_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO4569371.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         5764 a         5186 c         5281 g         5363 t
ORIGIN      
        1 aaggttaaga tcattgatgt atgatatgaa tttctcttct atcgtggctg atgaatatgg
       61 gataccacga caattgaatg aaaattcctt cgcaataacg acatcgttag ccgcaagtga
      121 aatcgaagat ttaatcaggc ttaaatgctt agacttaccg gatattgact ttgacctcaa
      181 cattatgaca gttgatgact atttccgtca gttttacaag tagccagtaa ggataagaag
      241 aacataaaaa atggcactat tctctaaaat attaattttt tatgtgattg gtgtgaacat
      301 atcctttgtc attatctggt ttatctcaca tgagaaaaca catattcgtt tacttagtgc
      361 attcctggtc ggaataacct ggccaatgag tctgcctgtg gcattacttt tttctctctt
      421 ttaggagctg tagttgagtg gacccgctgg ttatcgtgaa acgaagcgtc tctcctcaaa
      481 actggatttg cggaacgatg aacgaagcag tcgcctgact gcttcgttca ttaaagtgaa
      541 atttatttct gtgggcgcat cgccgggaag agaataacgt cgcgaatagt atggctgtta
      601 gtaaacagca taatcattcg gtcgatacca atacccagac cagcggttgg cggcagacca
      661 tattccagcg cagtcacgta atcttcgtca tagaacatgg cttcgtcgtc acctgcggct
      721 ttagcgttaa cctgttcctg gaaacgttca gcctgatctt ctgcgtcgtt taattcgcta
      781 aaaccattac cgatttcacg accaccgatg aagaattcaa aacggtcggt gatttccggg
      841 ttaacatcat tacggcgtgc cagcggtgac acttctgccg gatattccgt aataaaggtt
      901 ggctgaatca gatgtgcttc tgccacttca tcaaagatct ccgtgacaat acgtcccagc
      961 ccccagcttt tctctaccgt aataccgata gattcagcta atgctttagc agcatcaaaa
     1021 ttatccaggt cggccatatc ggtttctgga cgatattttt tgattgcttc gcgcatggtg
     1081 agtttttcaa acggtttgcc gaaatcaaac acatgctcgc cataagtgac tttagtggta
     1141 cccagaacct cttgtgccag ggtgcggaac agggactctg tcagttcaat caaatcgtgg
     1201 taatccgcat acgccatgta gagttccatc attgtgaact caggattatg gcgaacagaa
     1261 ataccttcat tacggaagtt acggttgatt tcgaataccc gttcaaaacc gcctacaacc
     1321 agacgtttca gatacagctc cggcgcgata cgcagataca tatctaaatc cagagcatta
     1381 tgatgggtaa taaacgggcg agcagatgcc ccacctggaa ttacctgcat catcggggtt
     1441 tctacttcca taaagccgcg cgcgaccatg aactggcgga tagcggccag aatttttgaa
     1501 cggacaacaa acgtttgacg ggatttatcg ttagcgatga ggtccagata acgttgacga
     1561 taacggactt cctgatcctg cagaccatgg aatttatctg gtaaaggacg tagtgcttta
     1621 gtcagcaggc gcaactcagt acagtgaatg gaaagctcac ccgtttgcgt cttaaacagc
     1681 gtaccgcggg caccgataat gtcacccaga tcccattttt taaactgatc gttataaaca
     1741 ccttctggca ggctatctcg tgcgacgtac agttgaatac ggccaccgac atcctgcaac
     1801 gttacaaagg aggctttccc catgatacga cgggtcatca ttcggccagc aaccgagact
     1861 tcaatgttta aggattccag ttcctggtta tccttcgcat caaactcttc gtgcaactgg
     1921 tcagaggtat ggtcgcggcg aaaatcattg ggaaacgcca caccttgctg acgtagtgcc
     1981 gccagttttt cgcggcgatt tctcagttca tcgttaaaat caatagcctc attggctccc
     2041 cgtgtttctt gttcagacat tttggttcct ctaaatccag ctttcaattt agcgtagata
     2101 aagagacaga tcggtacact gtattatctg cctcgactat taataactca gtttatctta
     2161 tcgaaactaa cgcttacctc aagttgatat cactaataaa taaccatcaa aatggtttaa
     2221 atgacttatt aatctcgatt tactagtgtg ataaactgaa agttaattta ttcataacca
     2281 attgttttta cgacagttaa tcgttgctct cctgtaccat attcgtcggc gtgctaaaca
     2341 ggaaacgggt agcaaaggcc aataccacga tgatcgcgac acaagccaac gtccactctc
     2401 ccatctgaga aaagaatcgc tggtaagctg caattgccat accgctaatt tgcgactctg
     2461 tcgtctgctg tgccactacg cctgccagcc agttggcgac cgcgcctgtt gccagcatat
     2521 aaatcccggt taatacgcca gacattttca gacgcgtgat ttgcgcaatc gccaccgggt
     2581 caataaagag ttcggcaaag cccattagcg ccagcccgga taccatcacg cccattgacg
     2641 cttgaccgtc agctgctgca tgtcgggcat caaatgccag caacataaag ccacaagcca
     2701 tcagcagtaa gccaaaggca aacttcagcc agacgcgcaa tgttgagttg ccgtggcttt
     2761 ctggcgacgc cagccaggcc agaacaaccc cagcgagcat caccgcaatt gcattcaccg
     2821 actggaatag tgcagtaggt acttcaatat tgaatgcctg acgattcaca aagcgatcga
     2881 taaacaagct gatggtactg ccgccctgtt gtgccagtac ccagaacaat gtcccgacaa
     2941 acatcaacaa tacaatttgc caaagagcac ggcggtgttc ggggaatttg atcatcatgc
     3001 gggcaatgat ttgtgcggca atgaggcaaa cgatcgccag caaatatccg gaccagtcgt
     3061 tctccagcag cagggtaaaa aatactgggg ctaaacagag catcaccact aaccagctcc
     3121 ataccggtaa ggcaaatttg acgctagtga gcgctttttt atccatacta cgcgtggact
     3181 ggaaatgacg atgcccgctt aagaaaatca acaaaccgat aaacatgccg ccacccgcaa
     3241 gggcaaagcc aacatgccat ccataccact gagcagccag gccgcaggcg attggggcgg
     3301 cgatagaacc gatattgccc gcagcataca gcagcgaaaa accgccatca cgtcgatgat
     3361 cgttctcgtc gtagagctcg ccaagcaaac agctgatgtt tgatttgaat aaaccgtagc
     3421 cacaaataat gattgccagc gccagataca ggctaaaggt tgaatttgta tcaataccca
     3481 gcaccacatg gccaagggtc attaacagcg cgccggcaat cactgcagtg cggttgccga
     3541 gcaggcggtc ggcaagccag ccgccgagaa taggggtaac gtaaaccaga gaagcatatg
     3601 cgctgaacag gctgatggca tggttatcat caaaaccaag ctgatgggtg agatagagaa
     3661 tgagtaaggc acgcatgccg taaaaactga agtactccca gatttggatc gccacgatat
     3721 agtatatcgc gcgcggctgt gagggtgttt tcatgtgttc tccttatgag caaaaaaggg
     3781 aagtggcaag ccacttccct tgtacgagct aattattttt tgctttcttc tttcaatacc
     3841 ttaacggtat agcggccatc agcctgacgg tatgcaccgt gaatatcggt ttcaaagccc
     3901 ggatagtgag cgccgatttc acacagcatc tgcaggaact ccagaaccgg acggctttct
     3961 tcggtgatca tttcacccgg cattaccaga ggaactcccg gcggatacgg aaggatcata
     4021 ttggcgttaa tacgaccgac catttcgtcg aggtaaactt cttcggtcat accgtgcagc
     4081 tctttctgga atgcagcata cggagtcatt accatcgtcg gcagcacttc aaatgcgcga
     4141 tacatcagat ccggcagatt gtggtgaaca atcagtttgt ggatattctg agccagttcc
     4201 tgaatacgca tgttttcata gaattcagga tcttcacgat acagagacgg cagcatgttt
     4261 ttcacacgca ggttcaggtc gaacgcacgt ttgaagtcag tcagagcacg cagcaggctc
     4321 agtgctttgg tcttatcgat accgatgctg aacaggaaca gcaggttata cggaccggtt
     4381 ttctcaacaa cgatgccatg ttcgtcgagg tatttcgcca cgatgctggc cggaatacca
     4441 aagtcgctca tggtgccgtc tttttccatc cccggagtca gcagggtgac tttgatcggg
     4501 tcaagataca tgtgctcgtt atcgatgttt ttgaagccgt gccaggtgct gtcagaacgc
     4561 agcggccagc attcagtcgt atcgatatga tccggctgcc atacatcaaa gaaccagcca
     4621 tcagattccg ttctcagacg tttgatctct ttacggaatt tgatcgcacg ttcaatggaa
     4681 ccgttgatca gacgcttacc tgcattgcct ttcatcatcg ccgcagcggt ttcagtggac
     4741 gccacgatac cgtagtgcgg agaagtggtg gtgtgcatca tgtaggcttc gttaaaggtt
     4801 tcttcgttta cgtcaccttt aacgtggatc atggaagcct gagagaacgc cgccagcagt
     4861 ttgtgagtgg actgggtttc gtaaatcact ttcccttcta cacggccacc gctcataccg
     4921 catttacctt cgtaaatcgg tgagaagttg gtgtaaggca cccacgcgga gtcaaagtgg
     4981 atggatttca catccagtgt tttcttgatg aagtcggtgt tgtacagcag accatcatag
     5041 gtagagttgg taattacagc atgtaccggc caggttgcgt ttggtgtttc tttcacgcgc
     5101 ttagcaatgg tagcgtgctg gaattcactc tgtgggatac caccaagaat accgtaagcg
     5161 ttacgggtcg ggcggaaata gattggcgta acatcgctca tcatcatcag gtgggtcagc
     5221 gatttgtggc agttacggtc aatcagaatg gtgctgcctg ccggagcaga gtacatacca
     5281 acaattttgt tcgcagtgga agtaccgttg gtcaccatgt agctgcggtc tgcgttaaag
     5341 acgcgagcga tatactgttc tgcttctttg tgtggaccac tgtgatccag cagagaaccc
     5401 agttcagata ctgaaatgga aatatcagat ttcatggtat tcggaccaaa gaaatcatag
     5461 aacaggctac ctaccgggct tttctggaat gcagtaccac ccatgtgacc aggagtacag
     5521 aaagtatatt taccttcacg aacatattta aacagtgctt tagtcagcgg aggcagaata
     5581 gtgttgatat attcgtcagt ggtctgcttg atcttgttag caatatcttc agcagcaccc
     5641 agcgcatatt caaagaagct aatctgtaaa cgcaggtcat tcaggcttac atcgagagtg
     5701 gaatacgtat tagcgaacgc gtacaacggc aggttctcgt tcattttgct aatttcttcg
     5761 cacagctcga gattatattt atcccagtca aaaataacgc cgcacagacg cgcattgttt
     5821 tcgatcagtt ttaataagtc gtcacggtcg ttcgggtaaa caatctggaa gttcagacgt
     5881 tcaagcgcgc gatgaagttc acggatgggt tcttctttaa aataaacccc catgtgattc
     5941 aatattgcaa taacgttcat agtcatatct ccaggtaaaa aaggcccctc ccaacacatg
     6001 ggacaaaatg aaaggaggag cctcggaaaa tacttttaat taatgtgcgt tagacgcggt
     6061 gtggttatcc attgagtggc tctggcgctc gtgcattttg cgagcgtaga acatcaggat
     6121 aatcaggctg acgatgaagg tacctgccag ctcgaaggag cttgcgccca tcagcgcgat
     6181 gaagcagaac acgcaaccca gtacagagca gatcaggctg acaaagttgc ggatgttaac
     6241 gccttcaaaa cgaatcaggt caacgcaaga gtagaagtac ggcagcatag tcagcagtac
     6301 tgcgataccg gtcagttcac cgaacaggtc agatgcttta ccaccggcag agttcatcag
     6361 agtgatgagg atcatcaggg cagtcatttt cactgcagcc agcagcagac cttttttcgg
     6421 aataccgttg ctgtcgactt caccataaac tttcgggaag ttaccgtcgt tagcggcacg
     6481 tacacctgcc tggcctacca acatcatcca ggagcccaga gaagtcaggc acgcaaaggc
     6541 ggtgaatgca gaaaccagcg gcgcagccca gttaccgagg atagttgaag cactgattgc
     6601 aaacggagca ccggaagccg ccattacaga agacggatac ataccggaaa gcacctgagt
     6661 cgcagcgatg taaacaatac ctgctaaacc agtacccagc atggttgcca gcggaacggt
     6721 acgtttcggg tttttaacca taccagtact tacagctgcg gattcaacac ccacgaaggc
     6781 ccacaggcag agcagaatac ttttaatgat cgcatgacca tcagtggtat ccgcagtatt
     6841 ccagttagct gcataagttg ccgcatcaaa ccaatgccag ccaacaatag cagtcatcac
     6901 cacaggaata agaaccagca ccagaccaat agtggttaaa cggcttaccc aggtaccgcc
     6961 gagcatattt acaaaggtaa atacccagac gatagcaata caggcgatac ccgccggaac
     7021 aggatcattt aatactggga agaaggtgga aagataagat acagcggtaa taccaatcgc
     7081 caggttacca atccagttag catggtaata aagaacacct gtctgaaaac caaatgcagg
     7141 agaaatttct ccggcataag caattgggcc accttgttgc gggttttttg ttgccagtcg
     7201 ggcatataca tacgccagcg acattgcacc aataatagag ataatccaac cccagatagc
     7261 aataccaccg atacttgcta ggttcgcagg taataatgca ataccgctcc ccatcatatt
     7321 accggcaaca acaccggtac aggcaaatag cccgatcttc ttggcagaac tcatgctctt
     7381 ctcctaattt catttttgaa tttggagtcc gggtcatgat gtataactat ttcctgacca
     7441 gaccaaactg gcgctaagat tactcacgaa aaaaggatta atcctaaaga ttaggtgaaa
     7501 taacacaaaa gtttctgtaa gtgagaactt gaggtttttt attaacacat caggatcgca
     7561 agttgatatc atgaaaagat aaacattcaa tgtttacaat agattgcgtg acgtcttctg
     7621 gttaaattta tgtaataaaa attatgcggc aaataaattg ccgcaacata ttataccaac
     7681 aggaacatac aaaaactcaa caacaaatat tcccgagcat gaatcaaccc gggggttact
     7741 tattctgaag cgagaaattt gtcgagataa ggtacaacat aaggaacaga agtctggaat
     7801 ataccatttt caatccagta aagggtgttt gcccctgggc gtaaattaaa ggcggtgaga
     7861 tatgcatcag ccgcttcccg gttcatcccc ttcatttcat aaaccttgcc aagcaataca
     7921 taatttagcc aggacatttc aagatcaatg ccagtattta tcgcctggta agactcatct
     7981 gttttacctt ttaccagagc actgaccgct tttatttgat atataatgga caggttattc
     8041 aattccggta gtgtaacaat gttatctatt tctgtgttca gtgctgctaa ttgtttttca
     8101 tctaaaggat gttgagaatg gcgcacgata tcaactaatg ctttttctgc tctcgcgtag
     8161 gtaaattctg gggatgattg aacaatctca cctaataatt cactggcacg gttcaatgat
     8221 ttatcatcgc catgcagtaa ataatcatgt gcctgataaa aattagttaa taacgcacca
     8281 cgatgaggca aaatttgctg gagcgtctcc tgcattcgtt gtggccacgg ttggtttaac
     8341 gcttttgata aactctccag taaatcattt tgaatcgcca gctgattacc gttagtgatg
     8401 acataacgtt tatccagcat ggttgaacca tctgcattgt ctaccaattt tatcgacata
     8461 aagcattgtt gagcacggta ttggcgctga ttaacaaacg caatagataa tgttttaccg
     8521 gaactgctcg gttcatcaat gttgtagttg attttgtcat gcaccataaa ggtggagaag
     8581 gtgttaagtg atgtcgccac caaatcaccc acgcctatcg cgtaagagag ctgatacggg
     8641 gaactccagc tgttacaact tttatttacc atattaatgt caatatcgcg tggattgagc
     8701 aaaatacgcg atttgctcat cggaagacgt gtatcaagac ttgaaaacgc taccagtgct
     8761 acacagatac ctaaggacaa caggaaaaaa aaccataccc aaaaggtagt gaatcgtttg
     8821 cttttaactg gggattgttc aggtggcgtt gcggtgtttt gaatgttaag actgtgggag
     8881 ggagaatctg tggcaggaac cgcctctggt atagggggag gcgaagatag cattatttcc
     8941 tctccctctt cttcgctgta ccagataacc ggcaccatta atttatagcc gcgctttggt
     9001 acagtagcga tatagacagg actatcttca tcattatctt ttaatgactt acgtagttct
     9061 gagatactct gcgtcacaac gtgattggtg acaatacttc tcttccagac attatcgata
     9121 agttcatccc tgctaagtac ttcgccactg tgttgagcaa agaaaaccag aaggtcgatt
     9181 aatctcggct caagggtaag ttgacgccca ttgcggctaa tttggtttat ggacggagta
     9241 acaagccatt cgccaacgcg aactacaggt tgttgcataa tagaaactca ttcgaaaagg
     9301 gaatgatgca atgataattg ccacaaccta tttttaccat ctatagatgg gtttatttac
     9361 atattattgg tgaatgcaag acgttatttt taccagccat aaacttcttg attacatggt
     9421 attacgaaag gattttactg agaaccagaa gtaatattcc ttaccatcaa aattcatcat
     9481 ctttgccaaa gaaaaatgtt cagaaaataa tccatggaaa attgtccgga gcgcttacta
     9541 ttttaatgga ttgttagtct ttgcatgagc aagcggactg atacatttct ctttgttctc
     9601 attcagaaaa tctcatcagt cggcgttcag accgatgttg cgctttatgg atgcgttacg
     9661 ctcctgatga cgtcatttga cgttcaacag catcacgggg ccgcacgaca tttcacgtta
     9721 gttagtgcta tagctcagga acaaattttc ccgaattggg atatgcccgc aaattgctgg
     9781 tgatgtgggg agaatcgggt tgagttcggt agaattgatt tggaggcaga acacttaaat
     9841 cgtggcgtcc tgaaacgaaa aacggacctc cgtggaggtc cgtttatatg aatttggtgc
     9901 ccggactcgg aattgaacca aggacacggg gattttcaat cccctgctct accgactgag
     9961 ctatccgggc aacggggcgc attaaaccgt aatctgcaca tctcgtcaac ctaatttcag
    10021 taaaagcgat tcaactgctt aagattgcgg caaatcgctt cttttctgtg tttctgtcag
    10081 gtcagtgcac caccctggcg gcagcgggcg aagcgcagga tatcttccgc cagccgatgt
    10141 gcggtgtcga catccgcctg gctacgattc accagcattc ggcttaaaca gccttccagc
    10201 accagttcca tctgctttgc taccatcgcc ggatcgtcaa cttccagcgt ggttaacagt
    10261 tcgtgggtga aatcgtaggc cgcgcttttt tgctgatcgg ccagttgatg aatagggtgg
    10321 ccaggatcgg gataaaacgt acaggcagcg ataaacagac agcccggata gcggttgttt
    10381 ttaacgcact ccgataacgc ctgataacgt gccagcagct tttgttcggc ggtttgcgtt
    10441 tcgtccagca tcagctgacg acgccagaca tctatctgtt ggctaagata acgcagcgca
    10501 tcgtagagga ttgcctcttt gtctggccag aagcggcgta gctcgtccag tggataatcc
    10561 acacgttcag caaccatctc cagcgtggtg ttggcaatcc cttgtaattc taataatttc
    10621 agggcttctc ccagtacatc ttcacgttgc acgctatttt cctccgtctt tcccactgca
    10681 agtgtcgttc acggttggcg atcgcgcaaa tgtgcgctga aggtttcagc atccataaag
    10741 cccgtgacgc gtgcttgtgg atgctcctgg ccttgtccgt caaaaaagag aattgtcggt
    10801 aggccaagga cattaagatg ctttaacagc gccacatctt gtgcgtcgtt ggcagtgacg
    10861 ttggcctgaa gtaataccgt gtctgctaac gctttttgca cctgcgggtc gctgaaggtg
    10921 tatttctcaa actctttaca ggcgacgcac cagtcggcat aaagatctaa catcaccggt
    10981 ttgcctttgg cttcaacgag cgcctgattt aactcatcta ccgttttgat ttgtgtaaag
    11041 ttgagatgcg tctgagtttg cgcggtatgc gtcgcaccaa atgcccaatc ctgaagtggg
    11101 cgcacgctaa ccaatgccgc tgcaagcagg attatttgca ccacgcgcat ccagccgcgt
    11161 ttggcctgta ggctggtgat aaaggcccag ccaaagaatg cgacacccag cgccgaccac
    11221 aagcgtaatc cccatacatc accaatcact cgctccagca ggaagaccgg cagtgcgagg
    11281 atcacaaaac caaacgcggt tttgacttgt tccatccacg ggccgctttt cggcagcaag
    11341 cggttaccaa agacggtaat tagcatcagc ggcaggccca tgcccaacgc atagagataa
    11401 agcgtgccgc cgcccagcca catgttcccg ctttgggcga tatacagcag aatcgcgcta
    11461 agcggtgcgg tggtgcatgg tgaacagatc agtccggcaa tcgtccccat aataaacact
    11521 ccgccgggtg agccgccctg ttggcgattg ctcatcagcg tgaggcgcgt ttgcagcgag
    11581 gaggggagtt gtagagtaag caagccaaac attgacatcg ccagcaaggt aaagacgatg
    11641 gcgaggccaa tgagcacgta tgggtgctgt agcgccgcct ggaactgtaa ccctgcggcg
    11701 gcaactacca gacccagcgc cgtgtaagtc agtgccatcc cctgcacata aataaaggtc
    11761 agcaacaatg ctctggcagt ggagagccgc tgtttaccac ccagcacgat gccagaaatc
    11821 agtgggtaca ttggcagcac gcatggcgta aaggcgatac caataccgat caacaacgcc
    11881 cagagcgcgg aaaagggcaa ttgcgcggtg ggctgctctt gctgcggaac agacacaggc
    11941 tgtgacgctg cgttgttggc gaccacttcg cttaacggaa cggttttggt ttctggcgga
    12001 taacagaaac cggcatcagc acagccctgg taggtgacag ttaacgtcgc tcccgcactc
    12061 gcctggttga tggttacggg aagcgtcagc cgatcgcggt aaatctcgct tttgccgtaa
    12121 aactcatctt catgccagac gccttgcggc agctgcacgt cggcaatttt cgcgtgttcc
    12181 ggcgtaatgc ggatctgttt acggtagagg tagtaaccgt ctttgatctg ccaggtcaga
    12241 ttaaggtcat gttggttttg ctgaaaatca aaagcaaagg cttgatccac ggggacaaat
    12301 tgtgaacgtc ccggcgcgtc gaataatccg gcaaaaacgg aagtgctgca aagtagcagg
    12361 atcagcgtaa agatgcgttg agccatgaga ggtaatctgt gtctccgtgt gtaacaggta
    12421 aaaccagaag ttccggggtt tgatatggat gatgagactt caggcattcc agcagtgcct
    12481 gctggtgaga tacggtagtt tttaaaatca tctgcacttc gtattcttgc tccagcttac
    12541 cttcccagta atagagagag gtagcgccgg ggatcaaggt cgcgcaggcc gccagttttt
    12601 ccgccagcac tttggcggct aaatcctggg ctgtcgcttc atccggtgcg gtacatagca
    12661 ccacgacaga cgcggtattc gaactttttt catcaagcat aaacacctcg caagaacaga
    12721 tggaaccgca agagagaggt cactatacaa cggacgggga aaggatgtta gtcggcgaga
    12781 aagaaagcgg ggcgtgaacg ccccgcgatt ggtcattaca gcatgaagct acccagcacg
    12841 aagccgaagc aaacggccag ggcaacaccc agagtacccg ggatgaagaa cggatggttg
    12901 aagacgaatt taccgatacg ggtagtaccc gtgtcatcca tctgtaccgc agcaaccagc
    12961 gtcgggtagg tcggcagaat gaacagacca gacaccgcag cgaaagaagc aacagcggtc
    13021 agcggagaaa cgttcagtgc cagagccatc ggcatcagtg cttttgcggt tgcagcctga
    13081 gagtacagca gagcagaagc aaagaagaag atgacggcca gcagccacgg atgaccctga
    13141 atcacttcac cagcggtatc tttgatccag tcgatgttgt tggaaacgaa agtatcgccc
    13201 agccacgcaa cacccaggat acaaatacag gcgctcatac ctgctttgaa ggtgctggag
    13261 ttgaggatgt tgtcggtatc gactttacag ataacggtgg tcagagttgc aacactgagc
    13321 atgatgatca ggattgcgtt ggtggtgttc atcagcggtt tttcaaccag acccatgctt
    13381 gggctgttga tgattgcata gataaccacg ccaactacgc ccagcaggaa cagccagacg
    13441 gacgtttttg caccggattt gatttcaatc tgcttttcac cgcgcagttc aaccaggccc
    13501 tcttccagac gcttgcgata aatcggatcg tcagagagtt tggagttgaa cagcatagtg
    13561 accaggaagg acatcaccag aaccgccagc agggtggacg ggatgaccac ggagagcaga
    13621 tggaggtagc tgatgccatg accttccatc acggaagaca tgtaaaccac tgccgctgag
    13681 attggcgatg cggtgatcgc aatctgcgcg gataccactg cagtagacag cggacggcaa
    13741 ggtttaacgc cttgttcctt cgcaacttca gcgataactg gcagtgtcgc cagagagatg
    13801 ttgccagtac cagcaaagat agtcaggaaa taggtcacga tcggtgcgag gatcgtgatg
    13861 tatttcgggt tacggcgcag cagcttttct gtctgatgaa ccagatagtc cagaccgcca
    13921 gcaacctgca tggcagaaat agcggcgata accgccatga taatggaaat gacatcgaac
    13981 gggatgttac cgggtttaac gccaatagcg gcaagaacca gcacccccaa tccgcctgca
    14041 aaaccaatac ctattccccc caatctggcg cccaagaaga tcgccagcaa aactatgatg
    14101 agttctacaa ctagcatatt agccttcctt gttttttaac aagttgatat tagattgtta
    14161 tttttaagtt actgctcaca agaaaaaagg cacgtcatct gacgtgcctt ttttatttgt
    14221 actaccctgt acgattactg ttcgctttca tcagtatagc gttttgcttt gtaagccggg
    14281 tgcatcagat tctgtacgga gaaaatatcg tcaagttccg cttcagtcaa cagaccgcgt
    14341 tccagaacga cttcacgtac actcttaccg gtttcggcac agattttacc cacgatgtca
    14401 ccgttgtggt gaccgatgaa cgggttcagg taagtaacga taccgataga gttgtaaacg
    14461 taaccttcgc acacttcttt gttagcagtg atgccgttaa tgcatttttc cagcaggttg
    14521 tagcaagcgt tggtcagaat gtgaacggat tcgaacatag cctggccaat gaccggctcc
    14581 ataacgttca actgcagctg acctgcttct gctgccatgg taacagtggt gtcgttaccg
    14641 atgactttga agcatacctg gttaaccact tccggaacaa ccgggtttac tttagctggc
    14701 atgatggaag agcccgcctg cagttccggc aggttgatct cgttcaggcc ggcacgtgga
    14761 ccagaagaga gcaagcgcag gtcgttacag attttggaca tcttcacagc caggcgtttc
    14821 agcgctccgt gaaccataac ataagcgccg cagtcagagg tcgcttcgat caggtcttca
    14881 gccggtacgc atgggaagcc agtaacttca gccagttttt tcactgccag cggagagtac
    14941 tctttcggcg tgttcagacc agtaccgatt gctgttgcgc caaggttaac ttccagcagc
    15001 agttcagcgg tacgttggat gtttttcact tcttctttca gcaggatgct gaaagcgcgg
    15061 aattcctgac cgagggtcat cggtactgcg tcctgcagct gggtacgacc cattttcagg
    15121 atgtcctgga attcgaccgc tttacgttca aagccttcac gcaattggtt aatcgcatct
    15181 accagcttaa tcagggaaga gtaaactgcg atacggaaac cggtcgggta ggcgtcgtta
    15241 gtggactgac atttgttaac atggtcgttc gggttcaggt actgatattc acctttctgg
    15301 tgacccatca gttccagacc gatattggcc agcacttcgt tggtgttcat gtttacggaa
    15361 gtacctgcgc cgccctggta gacgtctacc gggaactgat ccatgcattt tccgttgttc
    15421 aggacttcat cacatgcggc aatgatggca ttcgctacac ttttaggaat ggtttgcagc
    15481 tctttgtttg ccatagctgc ggctttttta accattacca taccgcgaac aaattcagga
    15541 atatcactga ttttgttgtt gctgatatag aagttttcaa tcgctctcag agtgtgaaca
    15601 ccatagtagg catcagctgg aacttccctg gtacccaaca gatcttcttc gatacgaatg
    15661 ttgtttgaca tgtgaacctt ctttttcaag ctgccaatga tttgctttaa acacacagaa
    15721 tatatgtggt ttcgaatgtt tttcgaccga cgattatccc ctgcatcgac cgaatatccg
    15781 agatcatatg ctgcttgagg atttctaccg taatctggat cactttaagt gtcggttttt
    15841 accccttaat tattaatttg tgaaatagat caccgctttg ggattactac caaaaatagt
    15901 tgcgcaaata tcttgaaatt ttgctaatga ccacaatata agctgaacgc gattcgcaac
    15961 ccattcaggt agccgggttt aaccggctgc tattacagga gaaacctttg cgctggttac
    16021 cttttattgc cattttcctt tatgtctata ttgagatttc aatctttatt caggttgccc
    16081 atgtattggg ggtattgctg actctcgtgc tggttatatt tacgtcagtt atcggtatgt
    16141 cactggtacg taaccagggc tttaagaatt tcgtgctgat gcagcaaaaa atggcggcgg
    16201 gtgaaaaccc agcggcggag atgattaaaa gtgtttcgct gatcattgcc ggtttgctgc
    16261 ttttattacc gggctttttc accgacttcc tcggtcttct gcttttattg ccgccggtgc
    16321 aaaagcatct gacagtgaag ttgatgccgc atttgcgctt ttctcgcatg ccaggcggcg
    16381 gttttagcgc cgggaccggt ggcggtaata cttttgatgg tgagtaccag cgaaaggatg
    16441 atgaccgcga ccgccttgat cataaagacg atcgccagga ttaatgtcga aacgccggat
    16501 tatgtggtta tgccattttc cggcgttttt cgttttggca gaaacagcca taaccccgcc
    16561 agcatgatca gcgcatagag acttttccag ccgaccattg ccagtaacag aacgcataac
    16621 agcccgccaa ccaccgccag tagtcgataa cgaccttgca ataatttaca gcctgccagc
    16681 atgcataaca gataaatcat aataaagatg ccattggcat aaataataag agcgtccaga
    16741 ttgatctcta aagcatgaat caccaacgtg ctcaccacgc agcagccgag caccgcattg
    16801 agggcattat tcgggatatg gcgagaagag aggcgtgcca ggtagtggtc aggattatgt
    16861 tgcgcctgcg accagaccag gcgggcgaag ctctgtatat aaatgttgag actggcaaag
    16921 caggccagat agccaattac gcaggcaatc cataacgctc ctacaccgaa cagttgtact
    16981 acaatttttg gaagcgatgc tgccgcggcc atttgttcac cataggcgtc gaagtgtaag
    17041 acgactaccg tacagcccca gtagactaat cctgccagca gcagaccaat catcaaagca
    17101 cgaggaaaat cacgttctgg atttttaaat tccgaggcaa gatgggcaaa tgcttccaga
    17161 ccgacaaaac accagaacat cactgataac gcagcaaata acccggtaag ttcgatattt
    17221 cctggcgcgg ggaaggggat actcgcaggt ttgatatcgc ccgcccacca gatagcgaca
    17281 atcagtgcga cgataagtcc ggcaataact gtttgtagat ttgcactgga actggcaccg
    17341 cgagtaccga tataccacac caacgccagc gtaccgagtt ctgccaacaa cagttgccag
    17401 ctatgccagc caaacattgc ctggccgaat ccggcagcaa tttgtagcgc ggcaggcaaa
    17461 cccacgggaa tgaccgataa aaacagccag ccggtgactc gctcaagccg cgaaccaaac
    17521 gccataccga cgaagtgcgc gacgccgcct gcgctgggat agtggcgacc cagaatcgca
    17581 aacacaatcg caatcgggaa cactaagata atcaaaatgg gccacgccca caggctgtta
    17641 ttgcccgcta ccagtgcagc taacgcagga acggcaaaca cgccagtgcc taataatgac
    17701 gtcgatagca ggccaatgcc ctgggccagc cccagttctt gtttgagtcc actcatgggt
    17761 tgatgtccgg ttgcgcccaa attttgggca actgcgtaga ttttcgatgg tagcacaatc
    17821 agattcgctt atgacggcga tgaagaaatt gcgatgaaat gtgaggtgaa tcagggtttt
    17881 cacccgattt tgtgctgatc agaatttttt ttctttttcc cccttgaagg ggcgaagcct
    17941 catccccatt tctctggtca ccagccggga aaccacgtaa gctccggcgt cacccataac
    18001 agatacggac tttctcaaag gagagttatc aatgaatatt cgtccattgc atgatcgcgt
    18061 gatcgtcaag cgtaaagaag ttgaaactaa atctgctggc ggcatcgttc tgaccggctc
    18121 tgcagcggct aaatccaccc gcggcgaagt gctggctgtc ggcaatggcc gtatccttga
    18181 aaatggcgaa gtgaagccgc tggatgtgaa agttggcgac atcgttattt tcaacgatgg
    18241 ctacggtgtg aaatccgaga agatcgacaa tgaagaagtg ttgatcatgt ccgaaagcga
    18301 cattctggca attgttgaag cgtaatcctc gcacgacact gaacatacga atttaaggaa
    18361 taaagataat ggcagctaaa gacgtaaaat tcggtaacga cgctcgtgtg aaaatgctgc
    18421 gcggcgtaaa cgtactggca gatgcagtga aagttaccct cggtccgaaa ggccgtaacg
    18481 tagttctgga taaatctttc ggtgcaccga ccatcaccaa agatggtgtt tccgttgctc
    18541 gtgaaatcga actggaagac aagttcgaaa acatgggtgc gcagatggtg aaagaagttg
    18601 cctctaaagc gaacgacgct gcaggcgacg gtaccaccac tgcaaccgta ctggctcagg
    18661 ctatcatcac tgaaggtctg aaagctgttg ctgcgggcat gaacccgatg gacctgaaac
    18721 gtggtatcga caaagctgtt accgctgcag ttgaagaact gaaagcgctg tccgtaccgt
    18781 gctctgactc taaagcgatt gctcaggttg gtactatctc cgctaactcc gacgaaaccg
    18841 taggtaaact gatcgctgaa gcgatggaca aagtcggtaa agaaggcgtt atcaccgttg
    18901 aagacggtac cggtctgcag gacgaactgg acgtggttga aggtatgcag ttcgaccgtg
    18961 gctacctgtc tccttacttc atcaacaagc cggaaactgg cgcagtagaa ctggaaagcc
    19021 cgttcatcct gctggctgac aagaaaatct ccaacatccg cgaaatgctg ccggttctgg
    19081 aagccgttgc caaagcaggc aaaccgctgc tgatcatcgc tgaagatgta gaaggcgaag
    19141 cgctggcaac tctggttgtt aacaccatgc gtggcatcgt gaaagttgct gcagttaaag
    19201 ctccgggctt cggcgatcgt cgtaaagcta tgctgcagga tatcgcaacc ctgactggcg
    19261 gtaccgtaat ctctgaagag atcggtatgg agctggaaaa agcaaccctg gaagacctgg
    19321 gtcaggctaa acgcgttgtg atcaacaaag acaccaccac catcatcgat ggcgtgggcg
    19381 aagaagctgc aatccagggc cgtgttgctc agatccgtca gcagattgaa gaagcaactt
    19441 ctgactacga ccgtgaaaaa ctgcaggagc gcgtagcgaa actggcaggc ggcgttgcag
    19501 ttatcaaagt aggtgctgct accgaagttg aaatgaaaga gaaaaaagca cgcgttgaag
    19561 acgccctgca cgcgacccgt gctgcggtag aagaaggcgt ggttgctggt ggtggtgttg
    19621 cgctgatccg cgtagcgtct aaactggctg acctgcgtgg tcagaacgaa gaccagaacg
    19681 tgggtatcaa agttgcactg cgtgcaatgg aagctccgct gcgtcagatc gtcctgaact
    19741 gcggcgaaga accgtctgtt gttgctaaca ccgttaaagg cggcgacggc aactacggtt
    19801 acaacgcagc aaccgaagaa tacggcaaca tgatcgacat gggtatcctg gacccaacca
    19861 aagtaacccg ttctgctctg cagtacgcgg cttctgtggc tggcctgatg atcaccaccg
    19921 aatgcatggt taccgacctg ccgaaaaacg atgcagctga cttaggcgct gctggcggca
    19981 tgggtggcat gggtggcatg ggcggcatga tgtaattgcc ctgcacctcg cagaaataaa
    20041 caaacccccg ggcagaaatg tctgggggtt tttcttttgg tcatctttct agtataagat
    20101 tcagacacgg acgacgcgag tggcgtccag ctcattgatt atggggaata acatgcacgt
    20161 aaaatactta gcagggattg tcggtgccgc gctactgatg gcgggttgta gctccagcaa
    20221 cgaattgagt gctgccggtc agagtgtacg cattgtggac gagcagccag gcgcagagtg
    20281 ccagctgatt ggtactgcga caggtaagca aagcaactgg ctttccgggc aacacggaga
    20341 agagggcggt tctatgcgcg gcgcagcaaa cgatctgcgc aaccaggcgg ctgcaatggg
    20401 cggtaacgtg atttatggca tcagtagccc gtcgcaggga atgttgtcca gttttgtccc
    20461 gacggatagc cagattatcg gccaggtata taagtgcccg aactgatgtg atggtgacaa
    20521 acgtataatt gccagatgcg cttcgcttat caggcctaca taaggcattg caactgattg
    20581 aatgcttgag aatttgtagg ccggataagg cgtttacgcc gcatccggca tgaccaacga
    20641 tcacgttatc agtcatttca ggctggatgg ctccagcctc gcattagcaa agatgagatt
    20701 atttcgcctg tggtgcagtt ttgctggtgg attgaacacg taagtgatgc agcgcgcgta
    20761 agcattgatc ctgggttagc ggttgggcgt tattcgccgc tacggtgcga acatagacct
    20821 gacatggcac acccacgagt tttttcagtc gtgggctgaa atcaagcatt cggcgagtga
    20881 tcgcctctat ttctttaccc ggcgttgctt cacgcgtttt caccactgcg ccgctaaatt
    20941 gttctttgcc actttcatct ttaaagcggt aaagcaccgc cagataatgg tcgaacaagt
    21001 tatgtttcca ttccggttgg gaataggtgt catattccag attgccattt tgctggcagt
    21061 caacgtcata taacggcagg aaatctaaca gtgaggtaat acgtaatgcc agttcacgca
    21121 tttccgcatt attaattgca tgaatgattg ctcgcgccag catttcaatc tgcaactcat
    21181 tgacgactaa ctcacatttg ctaccgtcat gtagcgttaa tacgaaggta agattttcgc
    21241 cttgattatc ggtaagttcc agcgtgttaa cccgacgatt aatatcggca tttttaagct
    21301 catcaaccag aatttctggg atgttttctg ccattttctt aatgacttta tcgcgagctt
    21361 gctcatactg aaggcgtgca gcagcatcca gtttatgctt ttgatgcaga cgactttcca
    21421 gcgcaatcag caggtcgcgc agttccatga cagacataaa gaacaacgat tctttattgc
    21481 gaggttcttt aattttcagc gccagtgcaa tgaaattatt actcttgcga ataacaccgg
    21541 tattaacacc cttgatgctt atggccatgt aggttctccc taaccatttc tcaa
//
LOCUS       DAZFBR010000081        21195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFBR010000081 DAZFBR010000000
VERSION     DAZFBR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.081"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            114..254
                     /locus_tag="ACHJZ2_003317"
                     /pseudo
     CDS             114..254
                     /locus_tag="ACHJZ2_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(279..593)
                     /gene="fliE"
                     /locus_tag="ACHJZ2_003318"
     CDS             complement(279..593)
                     /gene="fliE"
                     /locus_tag="ACHJZ2_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO4569372.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            808..2466
                     /gene="fliF"
                     /locus_tag="ACHJZ2_003319"
     CDS             808..2466
                     /gene="fliF"
                     /locus_tag="ACHJZ2_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO4569373.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSWRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2459..3454
                     /gene="fliG"
                     /locus_tag="ACHJZ2_003320"
     CDS             2459..3454
                     /gene="fliG"
                     /locus_tag="ACHJZ2_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO4569374.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3447..4133
                     /gene="fliH"
                     /locus_tag="ACHJZ2_003321"
     CDS             3447..4133
                     /gene="fliH"
                     /locus_tag="ACHJZ2_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO4569375.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4133..5506
                     /gene="fliI"
                     /locus_tag="ACHJZ2_003322"
     CDS             4133..5506
                     /gene="fliI"
                     /locus_tag="ACHJZ2_003322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO4569376.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5525..5968
                     /gene="fliJ"
                     /locus_tag="ACHJZ2_003323"
     CDS             5525..5968
                     /gene="fliJ"
                     /locus_tag="ACHJZ2_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO4569377.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            5965..7092
                     /gene="fliK"
                     /locus_tag="ACHJZ2_003324"
     CDS             5965..7092
                     /gene="fliK"
                     /locus_tag="ACHJZ2_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO4569378.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7197..7661
                     /gene="fliL"
                     /locus_tag="ACHJZ2_003325"
     CDS             7197..7661
                     /gene="fliL"
                     /locus_tag="ACHJZ2_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO4569379.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7666..8670
                     /gene="fliM"
                     /locus_tag="ACHJZ2_003326"
     CDS             7666..8670
                     /gene="fliM"
                     /locus_tag="ACHJZ2_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO4569380.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8667..9080
                     /gene="fliN"
                     /locus_tag="ACHJZ2_003327"
     CDS             8667..9080
                     /gene="fliN"
                     /locus_tag="ACHJZ2_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO4569381.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9083..9448
                     /gene="fliO"
                     /locus_tag="ACHJZ2_003328"
     CDS             9083..9448
                     /gene="fliO"
                     /locus_tag="ACHJZ2_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO4569382.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9448..10185
                     /gene="fliP"
                     /locus_tag="ACHJZ2_003329"
     CDS             9448..10185
                     /gene="fliP"
                     /locus_tag="ACHJZ2_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO4569383.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10195..10464
                     /gene="fliQ"
                     /locus_tag="ACHJZ2_003330"
     CDS             10195..10464
                     /gene="fliQ"
                     /locus_tag="ACHJZ2_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO4569384.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10472..11257
                     /gene="fliR"
                     /locus_tag="ACHJZ2_003331"
     CDS             10472..11257
                     /gene="fliR"
                     /locus_tag="ACHJZ2_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO4569385.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            11547..12182
                     /gene="rcsA"
                     /locus_tag="ACHJZ2_003332"
     CDS             11547..12182
                     /gene="rcsA"
                     /locus_tag="ACHJZ2_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO4569386.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTI
                     QISDQMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12226..12414)
                     /gene="dsrB"
                     /locus_tag="ACHJZ2_003333"
     CDS             complement(12226..12414)
                     /gene="dsrB"
                     /locus_tag="ACHJZ2_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO4569387.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12577..12804
                     /gene="yodD"
                     /locus_tag="ACHJZ2_003334"
     CDS             12577..12804
                     /gene="yodD"
                     /locus_tag="ACHJZ2_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO4569388.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13102..13917
                     /gene="yedP"
                     /locus_tag="ACHJZ2_003335"
     CDS             13102..13917
                     /gene="yedP"
                     /locus_tag="ACHJZ2_003335"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO4569389.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(13914..15608)
                     /gene="dgcQ"
                     /locus_tag="ACHJZ2_003336"
     CDS             complement(13914..15608)
                     /gene="dgcQ"
                     /locus_tag="ACHJZ2_003336"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO4569390.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(15779..15961)
                     /gene="yodC"
                     /locus_tag="ACHJZ2_003337"
     CDS             complement(15779..15961)
                     /gene="yodC"
                     /locus_tag="ACHJZ2_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO4569391.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVLGEGSRSAEEV"
     gene            complement(16040..16957)
                     /gene="yedI"
                     /locus_tag="ACHJZ2_003338"
     CDS             complement(16040..16957)
                     /gene="yedI"
                     /locus_tag="ACHJZ2_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO4569392.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            17130..18050
                     /gene="yedA"
                     /locus_tag="ACHJZ2_003339"
     CDS             17130..18050
                     /gene="yedA"
                     /locus_tag="ACHJZ2_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO4569393.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(18039..18509)
                     /gene="vsr"
                     /locus_tag="ACHJZ2_003340"
     CDS             complement(18039..18509)
                     /gene="vsr"
                     /locus_tag="ACHJZ2_003340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO4569394.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18490..19908)
                     /gene="dcm"
                     /locus_tag="ACHJZ2_003341"
     CDS             complement(18490..19908)
                     /gene="dcm"
                     /locus_tag="ACHJZ2_003341"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO4569395.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(19975..20670)
                     /gene="yedJ"
                     /locus_tag="ACHJZ2_003342"
     CDS             complement(19975..20670)
                     /gene="yedJ"
                     /locus_tag="ACHJZ2_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO4569396.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(20710..20990)
                     /gene="drpB"
                     /locus_tag="ACHJZ2_003343"
                     /pseudo
     CDS             complement(20710..20990)
                     /gene="drpB"
                     /locus_tag="ACHJZ2_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
BASE COUNT         5112 a         5621 c         5464 g         4998 t
ORIGIN      
        1 aaatcgctca tatccatagc ccggaacatg aataaaaaca tcaacaaaga taaaattatc
       61 cagaggttac tgttgcgtct gtggtcgtaa caacatccga cgtggtgatg tgtcaaatga
      121 gacactctga tgttcagggg gtttatcgtg tatacggatc gtggatggca gaaaactttc
      181 aggatcaggt atccatttca aaccagataa tgagtaagtt tgccccatcc atgccccacg
      241 cggtgagatc ggatgtcatt aacaacaggt tacacaacct atacctgcat gctcattact
      301 tcctgatatg ccgccaccag cttattacgc acctgaatcc ccatttgcat agaaactgag
      361 gctttttgca tatcggtcat cacatcgttt aacgccacgc cgggttcacc gagagtgaat
      421 ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta tgcgatcgag cgcggcgtgc
      481 agctgcccgg caaaactaat ggtcggttgc ggcagtgatt cctgcgcacg cgcactcatc
      541 gccgtagcct gtaactggct gataacccct tcaatcccct gtatcgctga cattttcatc
      601 tcctggataa tttctggtag caaagcctac cagtaagtca ataagacaaa ggcgctaaat
      661 agcaacaaaa aaacgggttt attggcggat agaaaaaaac gaaagcacaa ataatgggag
      721 cgtcaatttt tcgagtttgc tgacccggga gtgagtcttg ttccactttg ccaataacgc
      781 cgtccataat cagccacgag gtgcgcgatg aatgcgactg cagcccagac aaaatctctt
      841 gagtggctta atcgcctgcg tgcgaatccg aaaattccat tgattgttgc cggttccgcg
      901 gcagtggcgg tcatggtcgc actgatcctg tgggcgaaag cccccgacta ccgcacatta
      961 ttcagcaatc tttccgatca ggatggtggc gcaattgtca gccaactgac gcaaatgaat
     1021 attccttacc gcttcagcga agccagcggc gctattgaag ttccggcaga taaagttcac
     1081 gagctgcgcc tgcgcctggc gcaacaaggt ttgcccaaag gcggcgcggt cggtttcgaa
     1141 ctactcgatc aggaaaagtt tggtatcagc cagttcagcg aacaggtgaa ttatcagcgg
     1201 gcgctggaag gcgagctttc tcgtaccatc gaaactatcg gcccggtaaa aggggcgcgc
     1261 gtacatctgg caatgccgaa accgtcttta ttcgtccgtg aacaaaaatc cccttctgca
     1321 tcggtgacgg taaatctgtt acccggccgc gcactcgatg aagggcaaat tagcgccatt
     1381 gtgcatctgg tttccagcgc cgttgctggt ctgccgccgg gaaacgtcac gctggtggat
     1441 cagggcggac atctgttaac ccagtccaat accagctggc gcgatcttaa tgacgctcaa
     1501 ttgaaatatg ccagcgatgt cgaaggccgt attcagcggc gtattgaagc aatcctgtcg
     1561 cctattgttg gtaacggtaa tattcacgcc caggtcacgg cgcaactgga cttcgccagt
     1621 aaagaacaaa cggaagaaca gtatcgccct aatggtgatg aatctcaggc ggcgcttcgt
     1681 tcacgccagc ttaatgagag cgagcaaagc ggttccggtt atccgggcgg cgtaccgggg
     1741 gcgttgtcga atcaaccggc tcctgcgaat aacgcgccaa tcagcacgcc tccggcaaat
     1801 caaaataacc gccagcagca ggcgagcacc accagcaata gtgggccgcg tagcacacag
     1861 cggaatgaaa ccagtaacta cgaagtcgat cgcactattc gtcataccaa aatgaacgtg
     1921 ggcgatgtgc aacgtctgtc agtcgcggta gtggtgaatt acaaaacctt gccagacggt
     1981 aaaccattgc ctctcagcaa cgaacagatg aaacaaattg aagatctgac ccgcgaggcg
     2041 atgggctttt ctgaaaaacg cggtgactcg ctcaatgtcg ttaactcgcc gttcaatagt
     2101 agtgacgaaa gcggcggaga actgccgttc tggcaacagc aagcgtttat cgatcagtta
     2161 ctggctgccg gtcgctggtt gctggtactg ctggtggcgt ggctgctgtg gcgtaaagcg
     2221 gtacgcccgc agctaacacg tcgcgctgag gcgatgaaag ctgtacagca acaggcgcag
     2281 gcccgcgagg aagtggaaga tgcggtggaa gtccgcctga gcaaagacga acaactccaa
     2341 caacggcgcg ctaaccaacg tctgggggca gaagtcatga gccagcgtat ccgtgaaatg
     2401 tctgataacg atccgcgcgt ggtggcgctg gtcattcgcc agtggataaa taacgatcat
     2461 gagtaacctg acaggcaccg ataaaagcgt catcctgctg atgaccattg gcgaagaccg
     2521 ggcggcagaa gtgttcaagc acctctccca gcgcgaggtg caaaccctga gcgctgcaat
     2581 ggcgaacgtc acgcagatct ccaacaagca gctaaccgat gtgctggcgg agtttgagca
     2641 agaagctgaa cagtttgccg cactgaatat caacgccaac gattatctgc gttcggtatt
     2701 ggtcaaagct ctgggtgagg aacgtgccgc cagcctgctg gaagatattc tcgaaactcg
     2761 cgataccgcc agcggtattg aaacgctcaa ctttatggag ccgcagagcg ccgccgatct
     2821 gattcgtgat gagcatccgc aaattatcgc caccattctg gtgcatctga agcgcgccca
     2881 ggccgccgat attctggcgt tgttcgatga acgtctgcgc cacgacgtga tgttgcgtat
     2941 cgccaccttt ggcggcgtgc agccagccgc gctggcggaa ctgaccgaag tactgaatgg
     3001 cttgctcgac ggtcagaatc tcaagcgcag caaaatgggc ggcgtgagaa cggcagccga
     3061 aattatcaac ctgatgaaaa ctcagcagga agaagccgtt attaccgccg tgcgtgaatt
     3121 cgacggcgag ctggcgcaga aaatcatcga cgagatgttc ctgttcgaaa atctggtgga
     3181 tgtcgacgat cgcagcattc agcgtctgtt gcaggaagtg gattccgaat cgctgttgat
     3241 cgcgctgaaa ggggccgagc agccactgcg cgagaaattc ctgcgcaata tgtcgcagcg
     3301 tgccgccgat attctgcgcg acgatctcgc caaccgtggt ccggtgcgtc tgtcgcaggt
     3361 ggaaaacgaa cagaaagcga ttctgctgat tgtgcgccgc cttgccgaaa ctggcgagat
     3421 ggtaattggc agcggcgagg atacctatgt ctgataatct gccctggaaa acctggacgc
     3481 cggacgatct cgcgccacca caggcagagt ttgtgcccat ggtcgagtcg gaagaaacca
     3541 tcattgaaga ggctgaaccc agccttgagc agcaactggc gcaactgcaa atgcaggccc
     3601 atgagcaagg ttatcaggcg ggtattgccg aaggtcgcca gcaaggtcat aagcagggct
     3661 atcaggaagg actggcccag gggctggagc aaggtctggc agaggcgaag tctcaacaag
     3721 cgccaattca tgcccggatg cagcaactgg tcagcgaatt tcaaactacc cttgatgcac
     3781 ttgatagtgt gatagcgtcg cgcctgatgc agatggcgct ggaggcggca cgtcaggtca
     3841 tcggtcagac gccaacggtg gataactcgg cactgatcaa acagatccaa cagttgttgc
     3901 agcaagaacc gttattcagc ggtaaaccac agctgcgcgt gcacccggat gatctgcaac
     3961 gtgtggatga tatgctcggc gctaccttaa gtttgcatgg ctggcgcttg cggggcgatc
     4021 ccaccctcca tcctggcggc tgtaaagtct ccgccgatga aggcgatctc gacgccagtg
     4081 tcgccactcg ctggcaagaa ctctgccgtc tggcagcacc aggagtggtg taatgaccac
     4141 gcgcctgact cgctggctaa ccacgctgga taactttgaa gccaaaatgg cgcagttgcc
     4201 tgcggtacgt cgctacgggc gattaacccg cgctaccggg ctggtgctgg aagccaccgg
     4261 attacaattg ccgctcggcg caacctgtgt cattgaacgc cagaacggca gcgaaacgca
     4321 cgaagtagaa agcgaagtcg ttggctttaa cggtcaacgg ctgtttttaa tgccgctgga
     4381 ggaagtggaa ggtgtcctgc ccggcgcgcg ggtttatgcc aaaaacattt cggctgaagg
     4441 actgcaaagc ggcaagcagt tgccgctcgg tccggcgtta ttaggtcgcg ttctggacgg
     4501 cagcggtaaa ccgctcgatg gcctgccctc ccccgatacg acggaaaccg gtgcgctgat
     4561 taccccgcca tttaacccgt tgcaacgtac accgattgaa catgtgctgg acaccggcgt
     4621 gcgcccaatc aatgccctgc ttaccgttgg gcgtgggcag cgtatggggc tgtttgccgg
     4681 gtccggcgtt ggtaaaagtg tgctgctggg gatgatggca cgttacaccc gcgccgatgt
     4741 cattgtcgtg ggtttgattg gtgaacgtgg gcgcgaagta aaagatttta ttgagaatat
     4801 cctcggtgcc gaagggcgtg cacgatcagt ggtgattgcc gctccggcgg atgtgtctcc
     4861 gctcctgcga atgcaaggtg ccgcctatgc cacgcgcatt gccgaagatt ttcgcgatcg
     4921 tggtcagcat gtgttgctga ttatggactc cctcacccgc tacgcgatgg cccagcgtga
     4981 aattgcgctg gcgattggcg aaccacccgc caccaaaggt tatccaccgt cggtgtttgc
     5041 caaattaccg gcactggtcg agcgtgccgg aaatggcatt agcggcggcg gctcgattac
     5101 cgcgttttat accgtgctta ctgaaggcga tgaccagcag gaccccattg cagactccgc
     5161 gcgggcgatc ctcgacggtc acattgtgct gtctcgccga ctggcggaag ccgggcacta
     5221 tccggctatc gatattgaag cgtcgatcag ccgcgcaatg acggcgttga tcagtgagca
     5281 acattacgcg cgagtgcgca ccttcaaaca gctgttgtcg agttttcagc gtaaccgcga
     5341 tctggttagc gtcggcgcgt atgccaaagg cagcgatccg atgctcgaca aagccatcgc
     5401 cctgtggccg cagctggagg gctatttgca acaaggcatt tttgaacgcg cggactggga
     5461 agcgtctctc caggggctgg agcgtatttt cccgacagtg tcataaccca ggagataacg
     5521 gcagatggca gaacatggcg cgctggcgac cctgaaagat ctggcagaaa aagaggtaga
     5581 ggatgccgcg cgcctgctgg gtgaaatgcg tcgcgggtgt cagcaggcgg aagaacagct
     5641 caaaatgctg atcgattatc agaatgaata tcgcaataac ctcaacagcg atatgagtgc
     5701 cgggatgacc agcaaccgct ggatcaacta tcagcagttt atccagacgc tggaaaaagc
     5761 cattactcag catcgccagc aacttaatca gtggacgcag aaagttgaca ttgccctgaa
     5821 cagctggcga gaaaagaaac aacgtttgca ggcctggcag acactgcagg aacggcaatc
     5881 cacggcggca ctgcttgcag aaaaccgcct cgatcagaaa aagatggatg agttcgccca
     5941 gcgcgccgcc atgaggaaac ctgaatgatt cgcttagcgc ccttgattac cgccgacgtt
     6001 gacaccacca cattgcctgg cggcaaagcc agcgatgctg cacaagattt tctcgcgttg
     6061 ttgagcgaag cattagcagg cgagacaact accgacaaag cggcccccca gttgctggtg
     6121 gcaacagata agcccacgac aaaaggcgag ccgctgatca gcgatattgt ttccgacgcg
     6181 caacaagcta atttactgat ccctgtggat gaaacaccgc ctgtcatcaa cgacgaacaa
     6241 tccacatcaa caccgttaac caccgctcag acgatggcgt tggctgcggt ggctgacaaa
     6301 aatacgacaa aagacgaaaa agcggatgat ctgaatgaag acgtcaccgc aagcctgagc
     6361 gccctttttg cgatgttgcc gggttttgac aatacgccca aagtgactga tgtgccgtca
     6421 accgtgttac cggcagagaa accaacgctc ttcacaaaac tgacttctgc gcaactcaca
     6481 acagcacagc ctgatgatgc ccccggcaca ccagctcagc cattaacacc gcaggtagca
     6541 gaagcccaga gtaaagcgga agtcatcagc acgccttcgc cggtgactgc tgccgccagc
     6601 ccgctaatca ctccacacca gacacagcca ctgcccaccg tcgccgcgcc tgttttgagt
     6661 gcaccgctgg gttctcacga atggcaacaa tcattaagcc agcatatttc gctgttcacc
     6721 cgccaggggc aacaaagtgc agagttgcgt ctgcacccgc aggatttagg tgaagtgcaa
     6781 atctccctca aagtggatga taaccaggcg caaatccaga tggtttcatc gcatcagcat
     6841 gtacgcgccg ccctggaagc agcgctgccg gtactgcgta cgcagctggc cgaaagtggc
     6901 attcagttag ggcaaagcaa catcagtggc gaaagcttta gtggtcagca gcaggccgct
     6961 tcccaacaac agcaaagcca acgcacagta aaccatgaac ctctggcggg ggaagacgac
     7021 gatacgcttc cggttcccgt ctctttacaa gggcgcgtaa caggcaacag cggcgttgat
     7081 attttcgcct aacgtcagag gtagcaccgt aatccgcgtc ttttccccgc tttgttgcgc
     7141 tcaagacgca ggataattag ccgataagca gtagcgacac aggaagaccg caacacatga
     7201 ctgattacgc gataagcaag aaaagcaagc gatcgctttg gatcccgatt ctggtattca
     7261 ttaccctcgc ggcctgtgcc agcgcaggtt acagctactg gcattcgcat caggttgctg
     7321 ctgacgacaa agcgcagcaa cgcgtcgtgc cctccccggt cttctatgcg ctggatacct
     7381 ttacggtcaa tctgggcgat gcggatcgcg tgctttatat cggcataacc ctgcgcctga
     7441 aagatgaagc tacccgctcg cggctgagtg agtatttgcc ggaagtccgt agccgtttgc
     7501 tgttactgtt ttcgcgtcag gatgctgccg tactggcgac agaagaaggc aagaaaaacc
     7561 tgattgccga gattaaaacc acactttcca ccccgcttgt tgccgggcaa ccgaaacagg
     7621 atgtcaccga cgtgctgtat accgctttta ttctgcgata acgacatggg cgatagtatt
     7681 ctttctcaag ctgaaattga tgcgctgttg aatggtgaca gcgaagtcaa agacgaaccg
     7741 acagccagtg ttagcggcga aagtgacatt cgtccgtacg atccgaatac ccaacgacgg
     7801 gttgtgcgcg aacgtttgca ggcgctggaa atcattaatg agcgctttgc ccgccatttt
     7861 cgtatggggc tgttcaacct gctgcgtcgt agcccggata taaccgtcgg ggccatccgc
     7921 attcagccgt accatgaatt tgcccgcaac ctgccggtgc cgaccaacct gaaccttatc
     7981 catctaaaac cgctgcgcgg cactgggctg gtggtgttct caccgagtct ggtgtttatc
     8041 gccgtggata acctgtttgg cggcgatgga cgcttcccga ccaaagtgga aggtcgcgag
     8101 tttacccata ccgaacagcg cgtcatcaac cgcatgttga aactggcgct tgaaggctat
     8161 agcgacgcat ggaaggcgat taatccgctg gaagttgagt acgtgcgttc ggaaatgcag
     8221 gtgaaattta ccaatatcac cacctcgccg aacgacattg tggttaacac gccgttccat
     8281 gtggagattg gcaacctgac cggcgaattt aatatctgcc tgccattcag catgatcgag
     8341 ccgctacggg aattgttggt taacccgccg ctggaaaact cgcgtaatga agatcagaac
     8401 tggcgcgata acctggtgcg ccaggtgcag cattcacagc tggagctggt cgccaacttt
     8461 gccgatatct cgctacgcct gtcgcagatt ttaaaactga aacccggtga cgtcctgccg
     8521 atagaaaaac ccgaccgcat catcgcccat gttgacggcg tcccggtgct gaccagtcag
     8581 tacggcactc tcaacggtca gtatgcgtta cggatagaac atttgattaa cccgatttta
     8641 aattctctga acgaggaaca gcccaaatga gtgacatgaa taatccggcc gatgacaaca
     8701 acggcgcaat ggacgatctg tgggctgaag cgttgagcga acaaaaatca accagcagca
     8761 aaagcgctgc cgacgcggtg ttccagcaat ttggcggtgg tgatgtcagc ggaacgttgc
     8821 aggatatcga cctgattatg gatattccgg tcaagctgac cgtcgagctg ggccgcacac
     8881 gaatgaccat caaagagctg ttgcgtctga cgcaagggtc cgtcgtggcg ctggacggtc
     8941 tggcgggcga accactggat attctgatca acggttattt aatcgcccag ggcgaagtgg
     9001 tggtcgttgc cgataaatat ggcgtgcgga tcaccgatat cattactccg tctgagcgaa
     9061 tgcgccgcct gagccgttag tgatgaataa ccacgctact gtgcaatctt ccgcgccggt
     9121 ttctgctgcg ccactgctgc aggtgagcgg cgcactcatc gccattattg ccctgatcct
     9181 cgctgctgcc tggctggtaa aacggttggg atttgccccg aaacgcactg gcgttaacgg
     9241 tctgaaaatt agcgccagtg cttcgctggg cgcgcgtgaa cgggttgtgg tggtcgatgt
     9301 ggaagatgca cggctggtgc tcggcgttac cgcagggcaa atcaatctgc tgcataaact
     9361 tcccccttca gcaccaacag aagagatacc gcagaccgat tttcagtcgg tcatgaaaaa
     9421 tttgcttaag cgtagcggga gatcctgatg cgtcgtttat tgtctgtcgc acctgtcctt
     9481 ctctggctga ttacgcccct cgccttcgcg caactgccgg gtatcaccag ccagccgctg
     9541 cctggcggtg gacaaagctg gtcgctcccg gtgcagacgc tggtgttcat cacctcgttg
     9601 acgtttattc cggcaatttt actgatgatg accagtttca cccgcatcat cattgttttt
     9661 ggtttattgc gtaacgcgct gggaacaccc tccgcgccac ctaaccaggt attgctgggg
     9721 ctggcactgt ttttgacctt ttttattatg tcaccggtga tcgacaaaat ttatgtcgat
     9781 gcgtaccagc cattcagcga agagaaaata tcaatgcagg aggcgctgga aaaaggggcg
     9841 cagccgctgc gtgagtttat gctgcgtcag acccgtgagg cagatttagg gttgtttgcc
     9901 agactggcga ataccggccc gttgcaggga cctgaagccg tgccgatgcg cattttgctc
     9961 ccggcctacg tgaccagcga gttgaaaacc gcatttcaga taggcttcac gattttcatc
    10021 ccttttttga ttatcgacct ggtgatagcc agcgtgttga tggcattggg gatgatgatg
    10081 gttccccccg ccaccattgc tctgcccttt aaactgatgc tgtttgtact ggtggatggc
    10141 tggcaattgc tggtcggttc gctggcgcag agcttttaca gctagagagg caaaatgaca
    10201 cctgaatcgg tcatgatgat ggggactgaa gcgatgaaag tcgcgctggc actggctgcc
    10261 ccgctattgt tggtagcgtt ggtcacgggc cttatcatca gtattttgca ggccgccacg
    10321 cagattaacg aaatgacgct gtcgtttatt ccgaaaatca tcgccgtatt tatcgccatt
    10381 attattgccg gaccgtggat gctcaatctg ttgctggatt acgtccgcac cttgttcact
    10441 aacctgccgt atatcatcgg gtagccgtac tatgttgcag gtgacaagcg aacaatggct
    10501 atcctggtta agcctgtact tctggccgtt actgcgcgtg ctggcgctga tctccaccgc
    10561 gccgattctg agcgaacgca gcgtaccgaa acgggtaaaa ctgggtctgg caatgatgat
    10621 cacgttcgcc attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc
    10681 tctgtggctg gccgtgcagc agatcctgat cggcattgcg cttggtttta ccatgcaatt
    10741 tgcctttgcc gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt
    10801 tgcgacgttt gtcgatccgg ccagccatct taatatgccc gttttagcgc gtatcatgga
    10861 tatgctggcg ttactgctgt tcctgacatt taacggtcat ttatggttga tttcactgct
    10921 ggtcgatacc tttcacaccc tgccgattgg tggcgaaccg ttgaacagca atgcgtttct
    10981 ggcactcacc aaagcaggga gtttgatttt ccttaacggg ctgatgctgg cgttaccgct
    11041 cattactctg ctgctgacac tgaatctggc attaggttta cttaatcgta tggccccgca
    11101 attatccatt tttgttattg gatttccatt aactctgact gtcggcatct ctttaatggc
    11161 ggcattaatg ccgttaattg cgcctttttg cgaacattta ttcagtgaaa tttttaattt
    11221 gctggctgat attattagtg aattgccatt aatataattc cgtaacgttt atcatgttat
    11281 cctaaggatt atccgaaaaa taatacctac gaacatcttc caggatactc ctgcagcgaa
    11341 atatttgttt taagctcact cacatatcgc aacatttact ttactttaag acaattccag
    11401 gcaaattata caacacttta cgggatagta agtccgcctg aaaaatcgcg agagtggcgc
    11461 attaggtgac ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa ggggaattat
    11521 cgttacgcat tgagtgaggg tatgccatgt caacgattat tatggattta tgtagttaca
    11581 cccgactagg tttaaccggg tatctgttga gtagaggggt taaaaaaaga gaaatcaacg
    11641 acattgaaac cgttgatgac cttgccatag cttgtgattc acagcgccct tcagtggtgt
    11701 ttattaatga ggactgtttc atccacgatg cttctaacag tcagcgtatc aagctcatca
    11761 ttaatcaaca tcccaatacg ttatttatcg tttttatggc aattgccaat gttcattttg
    11821 atgaatatct attggtcaga aaaaatttat tgatcagttc taaatcgatt aaaccggaat
    11881 ctctcgacga tatccttggc gatatccttg gcgatattct gaaaaaagag acaacgataa
    11941 cctcgttttt aaatatgccg acgttatcat tgagccgaac cgaatcgagt atgttgcgaa
    12001 tgtggatggc aggtcaggga accattcaaa tctccgacca aatgaatatc aaagccaaga
    12061 ccgtttcatc gcataaaggt aatattaaac gtaagatcaa aacgcataat aaacaggtta
    12121 tctaccatgt cgtccgactg acggataatg tgactaatgg tatttttgtc aacatgcgct
    12181 aacacattct gactggtggt ttcccaccag tcaggctgaa ttagattact ctgctttctc
    12241 cacaaagata ccgtcctgat gccctgcttc attaaagaac cagatgccga gcgggtagtc
    12301 ttccagcgaa accaggtaca ttgtgccttc actaaactcc tcaactgcca gtaccacgcc
    12361 aggacgacgc ggaccgccat ccgttttgac tgttacccga tcattcacct tcatcgtttt
    12421 cctcctgtgg ctttgtgcca gtgtagaaca atttcgtttt tctggcagcg ccaggcgcgc
    12481 gcgagtgctg attttctcga cggtctatac ttaagagatg ccaggcggac ttaacgactg
    12541 gcggcaacaa cagagtaacg gttgcgagga aagatgatga aaaccgcaaa agagtacagc
    12601 gataccgcaa aacgtgaggt cagcgtcgat gtcgatgccc tgctggcggc gatcaatgaa
    12661 attagcgaaa gcgaagttca tcgcagccag aacgattctg aacacgttag cgtcgatgga
    12721 cgtgaatatc atacatggcg tgaattggcg gatgccttcg aactggatat tcatgacttc
    12781 agcgtctctg aagtgaatcg ttgaatgcac aataaaaaaa tcccgaccct gagggggtcg
    12841 ggatgaaact tgcttaagca agaagcactt aaaaaattcg ttacaccagg aaatctgatg
    12901 tgttcatcac cttatccgca atttttttcg ctgacaagaa aatattcgct atatgaatga
    12961 ttaacgctac ttatgaatag aaatgtgacg cacgtcaccc cttggtggct ctggcgtcag
    13021 cgggatcgct cccgaaaaaa tcatctttga taatttgtgc tgttttgaga atcatcctgg
    13081 taaggggagt actgcgcgac tatgttttca attcaacaac cactactggt ttttagcgat
    13141 cttgatggca ccctgctgga cagtcatagt tatgactggc aaccggcagc cccctggctc
    13201 agccgtttac gcgaagcaaa tgttcccgtc attctctgta gcagtaaaac atcagcggaa
    13261 atgctgtact tgcaaaaaat gttggggcta caaggtttac cgctgattgc agagaatggc
    13321 gcagtgatcc agcttgctga acaatggcag gagatagacg gttttccacg catcatctca
    13381 ggtattagcc atggcgaaat cagccaggtt ttaaatacgc tacgcgagaa agaacatttt
    13441 aaattcacga cttttgatga tgtcgacgat gcaaccatcg ccgaatggac gggattaagc
    13501 cgtagccagg cggcgctgac gcagcttcat gaggcgtcgg taacgctaat ctggcgcgac
    13561 agtgacgagc gtatggcaca atttaccgct cgtctgaacg aactgggctt acagtttatg
    13621 caaggtgcgc gcttctggca cgtcctggat gcctctgccg gaaaagatca ggctgccaac
    13681 tggattatcg cgacctatca acaattgtca ggcaaacgcc caaccacact tggcctgggc
    13741 gatgggccaa acgatgcgcc cttactggag gtaatggatt acgcggtgat tgtgaaaggg
    13801 ctaaaccgtg aaggggtgca tctgcatgat gaggatccgg cccgcgtctg gcgaacgcag
    13861 cgtgaaggac cggaaggatg gcgtgaaggg ctggaccatt ttttctccgc ccgttaagcg
    13921 ttatcgctcg cgaatacccg attgcgccca gcctgtttag cgagataaag ccgacggtcg
    13981 gccagtgact ggagttgttc aaaatcataa tcaccggttt cctcgctgct acttaccccc
    14041 aacgaggcac tgatgcgtat cgtcgtactc ttagcaatca acatctcttt ttcatttaac
    14101 ttcaggcgaa tacgttctgc gacttccgca gcctccgtca gactcgcgcc tggcagaatc
    14161 acacaaaact cctcaccacc gacccgcccg gcaacgtcct gcgcacgcaa ggaactgcta
    14221 attaatccgg cagcatgaga aagaacacgg tcgcccgcct gatgaccaaa gcggtcatta
    14281 atcgctttaa aatggtcaag atcgacctga atgacagaaa aaggatgttg gtgcgtctga
    14341 cacaatttcg cgagcggacg ggctttttcg aacagtgcgc cacggttata taaacgcgtt
    14401 aaggtgtcgt gccacgcctg ccactgcaac gagctttgca gaacatacat gttgctgacc
    14461 atccggcgaa tcacatacca ggagatgagt aacatggtgg taaagagcgc ccacagcagg
    14521 gttaatgcaa tgctgatact gccgaaatcg ccgcgcacgc cttcgcttaa cgtatggacg
    14581 cgcaccagca caccgtcgaa atggtccaga cgttcccagc taacatagcg actgtccata
    14641 cgaatgccgc cccgcgtgtc atgttccatt gcctgcgcca gcaaggccag ttcacgagga
    14701 tcaaaaatat tccctgttgg atggtcagga ttggaagagg tcaaaaatct cagcttactg
    14761 tcatagagct gatactcacc atcgaggttt ttatcgatgg cgtttcttaa aaattgctgc
    14821 atagtacgca cgggaatact catccccagc acgccatacc agtagttatt actgtctacc
    14881 ggaacactga cggtaacctg cggttcagta ttgctggcgt gttccggttg cgaagtgaac
    14941 cagcgtaccg cgcggtgacg attttctcgt tgtgaatggc cgataaacca gggttgggtg
    15001 acatagccgt aataacgcgt cgggacattg cgcgtaaaca aggtcggctg cgtcgaaacg
    15061 taaaatccgg cacgcgagac atacatcgcc tgttctacca tcgacgagga gttgtgtgcc
    15121 agtcgcagca agtaaccaac ttccagtgca gcggtaattt cattgtcgag gctttcattt
    15181 tcgcgagaca ggagattccc ctcgctgact aatgcatccg atacgccatt gactgacagg
    15241 gtgcgtcgtc ggttgagttc gatttgccag gcgtgctcgt cgcgatgctg ttcgaactcg
    15301 gttacagcat cacgcagaga ggtgaaatcc agtggcgcta cgagagcttc gcgcatgcca
    15361 ttacgcaaaa agatcagttt gtcgacgtta tactgcaaat gcttatcgag cgcgttggct
    15421 acgttttcca gatgattacg ctggctggag atataggcat cttccagcac cactacttca
    15481 cgccaggtca gcaaggtgga aaaaagcaat accacgataa agcagagatt aacgatatga
    15541 ccaggcccta ggcggcgtgc gagttttttc aaccagctct ggttttccat ttttgtctcg
    15601 tgctgcaccc tgacgactcc ccacctgctt ttttatgatt ctggcataac ctggcgatag
    15661 cgtaccgcaa aacagcgata attttataat ggtggcatga taagacgcga ctatcattca
    15721 ttgcaggcat tggcacacca ggccggatgt gatccggcct ggtcttaatg ataccctttc
    15781 aaacttcttc cgcagaacga ctcccctccc ctagaacaag ctcggtttca tgaaaagctt
    15841 cccgcttgac cccgtaaccg tcataccaac gacactcaac cataccgctg gagtatccag
    15901 tgacaatcat ccgcgggccg ccctctttaa ccgtaacttc ctcactaacc ataaagctca
    15961 tactcgcctc cttttttcga gtgaaacttg ttcaccttag ttgaagatgg cgaattttgc
    16021 ataatagcct gtgcgagtat taatgtacct gaccgcgcat tttcgctacg gctttcactc
    16081 ccagcaccac gatgccgccg atgataaatc caagaatcag atttaaaaca gtcggtaata
    16141 tcatcgccac cactgcactt tgctgcccgg cgaaatgttc aatggcgtga tgcagcggcg
    16201 caataccatg taccacaatc ccgccgccga cgaggaacat cgccagcgtg ccgacaatcg
    16261 ataacgcttt catcagccag ggcgcgataa tcaataatcc tttacctaat gcctgcatca
    16321 gcgcgctgga tttttccgcc agccaatacc ccaggtcatc aatcttaacg ataaccccta
    16381 ccagaccgta gacgcccacg gtcactacca gcgcgatgcc tgaaagcacc agcacctgat
    16441 taagcaacgg cgcttcggcc acaatcccca gcgtgatggc gacgatttcc gcagacaata
    16501 taaaatcggt acgaatcgcc ccttttattt tgtccttttc aaacttcagc ggatcctgcg
    16561 ccgccagctt ctccagacgc tgctggctct gcgccggatc ttctttatgt ttacgcgcct
    16621 ccagcatatg cagcactttc tctacccctt caaagcagag aaacgcgcca ccgatcatca
    16681 acaatggcgt aatcgcccac gggataaacg cactgatgat cagcgccagt ggcaccagaa
    16741 tcactttatt aatcagcgat cctttcgcca cgccccagac cacgggaagt tcccggttgg
    16801 cccgcacgcc tgaaacttgt tgcgcattga gcgataagtc atcccctaat acaccggcgg
    16861 ttttcttcgc cgccagtttg cccatcacgg agatatcgtc cagcagtgtg gcgatatcat
    16921 cgagcaacgt cagtaaacta ctcccggcca aaagcattcc ctctcatttt tattgttgaa
    16981 gggtgtaagt atgaagcaaa aacggcagct ctgatacttg cttcacaggt caacattttt
    17041 ttaacaatca agtgcaatta tatctctcgc cagcagaatg attttcacgt ttactatgaa
    17101 ccaccttttt atttccaccg tgagggatta tgcgtttccg ccagttgtta ccgctttttg
    17161 gcgcgctgtt tgcgttgtat atcatttggg gctcaaccta ttttgtcatt cggattggcg
    17221 tggaaagctg gcctccgtta atgatggcgg gtgttcgatt cctggcagcc ggtattttat
    17281 tgctggcatt tttactactg cgcggacaca aactcccccc gctacgtccg ctgctcaatg
    17341 ccgcgctgat tggcctgtta ttgctggctg tcggtaatgg catggtgacg gttgccgaac
    17401 atcaaaatgt tccttccggc atcgccgccg tagtggttgc aaccgtgccc ctctttaccc
    17461 tgtgcttcag ccgcctgttt ggcattaaaa cgcgcaaact ggaatgggtg ggtattgcca
    17521 ttgggcttgc cggaatcatc atgctcaata gcggtggaaa tttaagcggc aatccgtggg
    17581 gcgcgattct gattttaatc ggctcgatta gctgggcgtt tggctcagtt tatggctcgc
    17641 gcattacctt acctgtaggg atgatggcgg gtgcgattga gatgctggcg gcaggcgtgg
    17701 tgttaatgat cgcgtcgatg attgcgggtg aaaaactgac ggcgctccct tccctttcag
    17761 gcttccttgc ggtcggctat ctggcgctgt ttggttcgat tatcgccatc aacgcttata
    17821 tgtatttaat ccgtaatgtc agtccggctc tcgccaccag ctacgcttac gttaacccgg
    17881 tggtcgcggt cttgctgggt acgggactgg gtggagaaac actgtcgaag attgaatggc
    17941 tggcgctcgg cgtaattgtc ttcgcggtgg tactggtcac gttgggaaaa tatctctttc
    18001 cggcaaaacc cgtagttgct ccagttattc aggacgcatc aagcgagtaa atgtatcccc
    18061 tgcgtgtcga tctgcgcgct ggctccttcg ccgcagatcc actcttccag acgctcggta
    18121 agcgcttcat ccttcagctt ctcgcgccca cgtaacgcgc actcccagac aatcaatacg
    18181 cgccagccga gttcctgcaa gcgactgata tcgcggcgat cgcgctcaac atttttacct
    18241 atcttctcca gccagaactc ggttcgagtc gcaggcactt taaacagata gcagtgatga
    18301 tgatgccaga agcagccatg ggtaaatatc acgcagcgat attcatcaac gacaaaatcc
    18361 gggcgtccgg gcagactggc gtcctgaacg cgaaacgcca ggccctgccc ggttaacaga
    18421 ctggcgaggc gcttctcaat cgccgtatca cgcgtggcaa tcgcgcgcat atttttgctg
    18481 cgagtggcct tatcgtgaac gtcggccatg ttgtgcctct tgctgacgca acgccaccgc
    18541 ctgtttgatt tttggctcaa gcagttttgc caccgcggca aagaccggca cgaccaccga
    18601 gttaccgaac tggcgatagg cctgagtgtc cgaaaccgga atgcggaatt tcgcttctcc
    18661 cggcgcttca aaacccatta agcgcgcgca ttcccgaggc gttaaccgac gtggacgatg
    18721 ttgctgattc agcggatcgt caaagtcttt ctcacccgtg gccatatccc agccgcgatc
    18781 gattaaaatt tccgcgccat ctttgtaata acgcgcagac agcgtacggg tgacgctttg
    18841 cggattgttc ggataaacca ttccataacc gaagccgttt ccgcgcgcct gatgtttttt
    18901 cgcatatcga tagaggtact tccacagcac cggcgtcagg atatatttcg cctcgaccat
    18961 cgggtccaac agctgcgcca gcgtcactcg ctgtgcaggg aaacattcgc tgatatcacg
    19021 cagagtaaaa tcggctttca gattaagatc gcgacgaaaa cccaccagca cgatgcgttc
    19081 acggtgctgc ggcagaaaat gtttgccatc gatgattttc ggatcgtccg gcccgttatc
    19141 ttctgcatca gccacgtcat agcccagttc gtccagcgtc tgcatgatga tgcggaacgt
    19201 tttaccctgg tcgtggcttt tcaggttttt gacgttttcg agcacaaaca tcgccggacg
    19261 acgcgcgtcg ataatgcgca ccacatcaaa gaacagcgtg ccctgagtat cgcaggcaaa
    19321 accgtgcgcc cgcccgagcg agttcttttt cgatacgcca gccagcgaaa atggctgaca
    19381 agggaaaccg gccagtaaaa catcgtgttc aggaatgtgt tgacgaatat gttccgccgc
    19441 agcctcatca ctcacgcctt ctttatggct gagggtgatg tcgcggatat cttcattaaa
    19501 atgatgcgtc gccgggtcgc aataatgatt ggctttataa gtgcgtaccg catgtttgtt
    19561 ccattcgctg gtaaacacgc actgtccgcc aatcgattca aaaccgcgac ggatgccgcc
    19621 aattccggca aatagatcga taaagcgaaa cgcataatgc ggatgatgtg ccggtggttt
    19681 gggtaacaac gtttgcagat gggcgaactc tttctcactt aaacggtgcc atgccgagtc
    19741 attcgccagc gcacgtttta aaattgccgc gctccagtga ttctcaccca caccattaag
    19801 ctgagccacc agcgttttaa catcataaat ttgcagcagt ttctccagca ttgcctgtgc
    19861 ggcattcccg gtgctgtatg aatcggttac tgatatattt tcctgcatag atttaaccgg
    19921 ccatctgaga tggtcgacag gttaacataa ttacagccat ttagggccac gcgctcagcc
    19981 cgcgggtgaa aacgcatcta tcaccttgtg atcgacacct tcattctccc ccgccagttc
    20041 ggcactgagc ttcgccataa actcgactaa aaaatgcgcg ttgtgctgcg ccagctgctt
    20101 gcctcttacg gtttgcatga tttgcggtag cttcaacaac ttagtctgga aatgatccag
    20161 cgcgtagcgt ttatcatcaa gcgggcgatg ctgtgcgagc ggatcttcgg catcaaacag
    20221 cgccacgccc aatgcccctg aaacggcaaa cacgcgcgcc aggccaatcg cccccagggc
    20281 ttccagccga tccgcatcct ggacgatttt agcttccgtt gttaaggggg cgatttgcgc
    20341 gctgaaactg tgagcggcaa tggcatgaca aacggcctcg attttctccg ccggaaattg
    20401 cacgaactct tcacggagca ggcgacgcgt ctcttctgcc gccaggattg aagaactctg
    20461 ccgttgcgga tgatttttcg ccaggctcac aatatcgtgg aaataacagg cggttaaaat
    20521 caccagcata tcgacgtcat catctgccgc cagcttctgt gccgtagccc agacgcggcg
    20581 aaaatgacac acgtcatggg ccgcgtcctg gtgctgatga tggtttttca accagttttc
    20641 gaactgtgcc tgccagtgtt gtaagtccat acgcgctccg ttgccaaaga gtcgccacct
    20701 tagcagtttt tattcatagc gtctgctacg tgcgggacga ggtcgtgtgt accaggcaat
    20761 gctgcctaac aatgccccga ccagagccat aaataaaaat ccgaccagtg ccctaaccac
    20821 tttcccgcgg tagagcctaa atcgctttca aatcccgaca cgggggcatc ggggatccgg
    20881 ctcatcaccc agatgtaacg cgccattgcc cagacgaaat agccacacca tgtataaaaa
    20941 gcccacaaag ccagtttgcc gccagggctg cgagagagtc tctcttccat ctttgtcgaa
    21001 ccgtattcca ttttgagata acccggaaaa ttcagactta gtactattac gtgataaaag
    21061 tcacattttt ccacattgga tgagccagtc aagactcact cattttttga acaaatagat
    21121 aatttttgcg caacggcagt gaatacagga aaaatcgttt tgagcgattt tatttatctt
    21181 tgagatcggc tttat
//
LOCUS       DAZFBR010000082        20980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFBR010000082 DAZFBR010000000
VERSION     DAZFBR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.082"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            93..368
                     /gene="ytcA"
                     /locus_tag="ACHJZ2_003344"
     CDS             93..368
                     /gene="ytcA"
                     /locus_tag="ACHJZ2_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO4569397.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
     gene            387..1418
                     /gene="mdtN"
                     /locus_tag="ACHJZ2_003345"
     CDS             387..1418
                     /gene="mdtN"
                     /locus_tag="ACHJZ2_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO4569398.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1418..3469
                     /gene="mdtO"
                     /locus_tag="ACHJZ2_003346"
     CDS             1418..3469
                     /gene="mdtO"
                     /locus_tag="ACHJZ2_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO4569399.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCAIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            3466..4932
                     /gene="mdtP"
                     /locus_tag="ACHJZ2_003347"
     CDS             3466..4932
                     /gene="mdtP"
                     /locus_tag="ACHJZ2_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO4569400.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            5130..7277
                     /gene="fdhF"
                     /locus_tag="ACHJZ2_003348"
     CDS             5130..7277
                     /gene="fdhF"
                     /locus_tag="ACHJZ2_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5547..5549,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFO4569401.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7371..8060
                     /gene="yjcO"
                     /locus_tag="ACHJZ2_003349"
     CDS             7371..8060
                     /gene="yjcO"
                     /locus_tag="ACHJZ2_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO4569402.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(8238..9551)
                     /gene="gltP"
                     /locus_tag="ACHJZ2_003350"
     CDS             complement(8238..9551)
                     /gene="gltP"
                     /locus_tag="ACHJZ2_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO4569403.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9893..10489)
                     /gene="nrfG"
                     /locus_tag="ACHJZ2_003351"
     CDS             complement(9893..10489)
                     /gene="nrfG"
                     /locus_tag="ACHJZ2_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO4569404.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10486..10869)
                     /gene="nrfF"
                     /locus_tag="ACHJZ2_003352"
     CDS             complement(10486..10869)
                     /gene="nrfF"
                     /locus_tag="ACHJZ2_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO4569405.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10862..12580)
                     /gene="nrfE"
                     /locus_tag="ACHJZ2_003353"
     CDS             complement(10862..12580)
                     /gene="nrfE"
                     /locus_tag="ACHJZ2_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO4569406.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            complement(12600..13556)
                     /gene="nrfD"
                     /locus_tag="ACHJZ2_003354"
     CDS             complement(12600..13556)
                     /gene="nrfD"
                     /locus_tag="ACHJZ2_003354"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO4569407.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13553..14224)
                     /gene="nrfC"
                     /locus_tag="ACHJZ2_003355"
     CDS             complement(13553..14224)
                     /gene="nrfC"
                     /locus_tag="ACHJZ2_003355"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO4569408.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(14221..14787)
                     /gene="nrfB"
                     /locus_tag="ACHJZ2_003356"
     CDS             complement(14221..14787)
                     /gene="nrfB"
                     /locus_tag="ACHJZ2_003356"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO4569409.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14832..16268)
                     /gene="nrfA"
                     /locus_tag="ACHJZ2_003357"
     CDS             complement(14832..16268)
                     /gene="nrfA"
                     /locus_tag="ACHJZ2_003357"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO4569410.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16661..18619
                     /gene="acs"
                     /locus_tag="ACHJZ2_003358"
     CDS             16661..18619
                     /gene="acs"
                     /locus_tag="ACHJZ2_003358"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO4569411.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18820..19134
                     /gene="yjcH"
                     /locus_tag="ACHJZ2_003359"
     CDS             18820..19134
                     /gene="yjcH"
                     /locus_tag="ACHJZ2_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO4569412.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            19131..20780
                     /gene="actP"
                     /locus_tag="ACHJZ2_003360"
     CDS             19131..20780
                     /gene="actP"
                     /locus_tag="ACHJZ2_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO4569413.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         4985 a         5720 c         5528 g         4747 t
ORIGIN      
        1 tttattcatc aagaaaatag ccatactgac gcattcaatg gatattgata ttcactacag
       61 atattgttcg cagcagacag gatgtattta atatgccaac agttctctct cgcatggcga
      121 tgcaactcaa aaaaacagcc tggataattc ccgtcttcat ggtttcggga tgctcattat
      181 ctccggcaat cccggtgatc ggcgcttatt atcccagctg gtttttctgc gccattgcca
      241 gccttatatt gacgctcatc acgaggcgag ttattcagcg ggcaaatatc aagctggcat
      301 ttgtcggaat tatttatacc gccctttttg ctctctacgc catgctgttc tggctggcat
      361 ttttctaatc tatttgagac gcgctcatgg aaagtacgcc gaaaaaagct cctcgcagta
      421 aattccctgc tctgttagtg gttgcgttgg cgctggttgc ccttgttttc gttatctggc
      481 gcgtagacag tgcgccatca actaatgacg cttacgtgtc agcagatacc attgatgtgg
      541 tgccggaagt cagcggccgc attgtagaac tggcggtcac cgacaaccag gcagtcaaac
      601 agggcgattt gctgttccgc attgacccgc gcccgtacga agccaatctg gcgaaatctg
      661 aagcctccct cgcggcactg gataagcaaa ttatgctcac ccagcgtagc gttgacgcgc
      721 aacagtttgg tgccgactcg gttaacgcca cggtagaaaa agcccgtgcc gccgctaaac
      781 aggccacaga tacattacgc cgcaccgagc cattactgaa agaaggtttt gtctcagcgg
      841 aagacgttga ccgtgcgagg accgcgcagc gcgctgcaga agccgatctt aatgctgtat
      901 tgttgcaggc gcagtcagcc gccagcgccg tcagcggcgt ggatgcatta gttgcccagc
      961 gtgcggcggt cgaagcggat attgccctga ccaaactgca tctggaaatg gcgaccgttc
     1021 gcgcaccgtt tgatggccgg gtcatttccc tcaaaacctc cgtcgggcaa tttgcttctg
     1081 ccatgcgccc tatttttacc ctaatcgaca ctcgtcactg gtacgtgatc gccaacttcc
     1141 gcgaaaccga tctgaaaaat attcgctcag gtacacctgc aacgattcgc ctgatgagtg
     1201 acagcggcaa aactttcgag ggtaaagtgg attcaattgg ctacggcgtg ctaccggatg
     1261 acggcggcct ggtgctgggc ggcctgccga aagtttctcg ttctattaac tgggtccgtg
     1321 ttgcccagcg ttttccggtc aaaatcatgg ttgataaacc tgacccggaa atgttccgca
     1381 tcggcgcttc ggcagtcgct aatcttgagc cgcaataatg agcgcgctca actccctgcc
     1441 attaccggtg gtcaggctgc tggcgttctt tcatgaagag ttaagcgagc ggcgaccagg
     1501 tcgcgtgccg cagaccgtgc aactctgggt aggctgcctg ctggtgattc tgatctcgat
     1561 gacttttgag atcccttttg tggcgttatc gctggcagtg ctgttttacg gtattcagtc
     1621 gaacgcgttt tacaccaaat ttgtcgcgat cttgtttgtg gttgccacgg tgctggagat
     1681 cggcagcctg tttttgatct acaaatggtc atacggcgaa ccgttgatcc gcttgatcat
     1741 cgccgggccg atcctgatgg gctgcatgtt tttgatgcgc acccatcggt tgggactggt
     1801 ctttttcgct gtcgccattg tcgccattta tgggcaaacc ttccccgcca tgctcgacta
     1861 tccggaagtg gtcgtgcgct taacgctgtg gtgtatcgtt gttggcctct acccgacgtt
     1921 attaatgacg ttaatcggcg tactgtggtt tcccagtcgt gccatttcgc aaatgcatca
     1981 agcgcttaat gatcggcttg atgatgccat tagccacctg acggacagcc tcgcaccgct
     2041 acccgaaacg cggattgaaa gagaggcgct ggcgctgcaa aaactcaatg tcttttgcct
     2101 cgcggacgat gccaactggc gaactcaaag cgcatggtgg caaagctgcg tggcaacggt
     2161 aacctacatt tactcgacgc tgaatcgcta cgatcccacc tcttttgctg attctcaggc
     2221 aattattgaa ttccgacaaa aattagcttc agaaatcaac aagctgcagc atgccattac
     2281 cgaaggtcag tgctggcaaa gcgactggcg gatcagtgaa agtgaagcga tggcggcacg
     2341 ggaatgtaac ctggagaata tctgccagac gttgttacaa ctgggtcaga tggacccgaa
     2401 tacgccgcca acgcccgccg ccaaaccgcc atcaatggtc gctgatgctt ttaccaatcc
     2461 agactatatg cgctacgcgg taaaaacgct gctcgcctgt ttgatctgtt acaccttcta
     2521 cagcggcgtg gactgggaag gcattcacac ctgtatgctg acctgcgcga tcgtcgctaa
     2581 tccgaatgtc ggttcatcgt accagaagat ggtgctgcgt tttggcgggg ccttttgcgg
     2641 cgcgattctg gcgctgttat tcacgctact ggtcatgccc tggctggaca atattgtcga
     2701 attgctgttt gtgctggcac cgattttcct gttgggcgca tggattgcca ccagctctga
     2761 acgctcttct tatatcggca cacagatggt ggtcaccttc gcgctcgcca cgctcgaaaa
     2821 cgtttttggt ccggtgtacg acctggtgga aattcgcgat cgcgccctgg gtatcatcat
     2881 tggtaccgtg gtgtctgcgg tgatttacac ctttgtctgg cctgaaagtg aagcgcgcac
     2941 gctgccgcaa aaactggctg gcgcgctggg tatgctaagt aaagtaatgc ggatcccacg
     3001 ccagcaggaa gtcacggctc tgcgcactta tctgcaaatt cgtataggtc tgcatgcggc
     3061 gtttaatgcc tgtgaagaga tgtgccaacg cgtggcgctg gagcgtcaac tggacagcga
     3121 agaacgcgcc ttactgattg aacgttcgca aacggttatt catcagggcc gcgatcttct
     3181 tcacgcctgg gatgccacct ggaactcggc gcaggcgctg gataacgcac tacagccgga
     3241 caaagcaggt cagtttgccg acgccctgga aaaatacgct gccggtctgg caaccgcact
     3301 cagccgttct cctcaaataa cgcttgaaga gacacccgcc tcgcaggcca tcctgcccac
     3361 cttattaaaa caggagcaac acgtctgcca gcttttcgcc cgcttgccag actggacagc
     3421 cccggcatta acgcccgcca cggaacaggc acaaggagcc acgcaatgat caatcgtcaa
     3481 ctttcacgtc tgctgttgtg cagcattctc ggcagcacga cgctgatttc cggctgtgcc
     3541 ctggtacgca aggattctgc gcctcatcaa cagctcaaac cggaacaaat caaactggcc
     3601 gatgatattc atcttgccag ctccggctgg ccgcaggcgc agtggtggaa acaactcaat
     3661 gacccgcagc tggatgcgct gatccaacgg acgctaagtg gttcacacac cctcgccgaa
     3721 gcgaaactgc gggaagaaaa agcgcagtcc caggccgatt tgttagatgc cggttcacaa
     3781 ttacaggtcg cagcgttagg gatgctcaac cgccaacgcg tctcggcgaa cggcttttta
     3841 agcccttatg cgatggatgc gccagcactg ggtatggacg ggccgtacta tacggaagcc
     3901 acagtcggtt tgtttgccgg actggatctt gatttgtggg gtgtgcatcg ctcagcggtt
     3961 accgccgcca ttggcgcgca taatgccgcg ctggcagaaa ccgcagcagt agagctatcg
     4021 ctgaccacgg gcgtagcgca gctttattac agtatgcagg ccagctatca gatgctcgat
     4081 ctgttagaac aaactcgcga tgtgattgat tacgcggtga aagcgcacca aagtaaagtg
     4141 gcgcacggtc tggaagcgca agtgcctttc cacggcgcgc gggcacagat tctggcggtc
     4201 gataaacaaa ttgctgccgt taaagggcaa atcaccgaaa cgcgagaatc tctgcgtgca
     4261 ttgattggcg cgggagccag cgatatgccg gagatcaaac cagtggcatt accacgagtc
     4321 cagaccggta ttccggcgac actctcttat gagttgctcg ccagacgccc ggatctgcaa
     4381 gccatgcgct ggtatgttca ggcgtcatta gatcaggtgg attccgcgcg ggcgctgttc
     4441 tacccaagct ttgatatcaa agcgtttttc ggtctggact ccatccacct ggatacctta
     4501 ttcaaaaaaa ccagtcgcca gttcaacttc atcccgggtc tgaaattgcc gctgtttgac
     4561 ggtggacggt tgaatgccaa tctcgaaggc actcgcgccg ccagcaacat gatgattgaa
     4621 cgttacaacc agtcagtact gaacgcggtg cgtgacgttg ccgtcaacgg cacgcgtctg
     4681 caaacgctca acgacgagcg agaaatgcag gctgaacgcg tggaagcaac gcgcttcacc
     4741 cagcgcgctg ccgaggccgc ctatcagcgc ggcttaacca gccgcttaca ggctaccgaa
     4801 gcccggttgc cggtgcttgc cgaagagatg tcattactga tgctggacag ccgccgggtg
     4861 atccaaagca ttcagttgat gaaatcgctg ggcagcgggt atcaggcggc tcccgtcgtc
     4921 gagaaaaaat aaaatgtctg ccgtgtgatg gctgtcacgc ggtatttcgt ttcgtcacgt
     4981 caaaactgac gacagcctgt ttttcgtcag agttttgaat aaatagtgcc cgtaatatca
     5041 gggaatgacc ccaaataaaa tgtggcataa aagatgcata ctgtagtcga gagcgcgtat
     5101 gcgtgatttg attaactgga gcgagaccga tgaaaaaagt cgtcacggtt tgcccctatt
     5161 gcgcatcagg ttgcaaaatc aacctggtcg tcgataacgg caaaatcgtc cgggcggagg
     5221 cagcgcaggg gaaaaccaac cagggtaccc tgtgtctgaa gggttattat ggctgggact
     5281 tcattaacga tacccagatc ctgaccccgc gcctgaaaac ccccatgatc cgtcgccagc
     5341 gtggcggcaa actcgaacct gtttcctggg atgaggcact gaattacgtt gccgagcgcc
     5401 tgagcgccat caaagagaag tacggtccgg atgccatcca gacgaccggc tcctcgcgtg
     5461 gtacgggtaa cgaaaccaac tatgtaatgc aaaaatttgc gcgcgccgtt attggtacca
     5521 ataacgttga ctgctgcgct cgtgtctgac acggcccatc ggttgcaggt ctgcaccaat
     5581 cggtcggtaa tggcgcaatg agcaatgcta ttaacgaaat tgataatacc gatttagtgt
     5641 tcgttttcgg gtacaacccg gcggattccc acccaatcgt ggcgaatcac gtaattaacg
     5701 ctaaacgtaa cggggcgaaa attatcgtct gcgatccgcg caaaattgaa accgcgcgca
     5761 ttgctgacat gcacattgca ctgaaaaacg gctcgaacat cgcgctgttg aatgcgatgg
     5821 gccatgtcat tattgaagaa aatctgtacg acaaagcatt cgtcgcttcc cgtacagaag
     5881 gctttgaaga gtatcgtaaa atcgttgaag gctacacgcc ggagtcggtt gaagatatca
     5941 ccggcgtcag cgccagtgag attcgtcagg cggcacggat gtatgcccag gcgaaaagcg
     6001 ccgccatcct gtggggcatg ggtgtaaccc agttctacca gggcgtggaa accgtgcgtt
     6061 ctctgaccag cctcgcgatg ctgaccggta acctcggtaa gccacatgcg ggtgttaacc
     6121 cggttcgtgg tcagaacaac gtgcagggtg cctgcgatat gggcgcgctg ccggatacgt
     6181 atccgggata ccagtacgta aaagatccgg ctaaccgcga gaaattcgcc aaagcctggg
     6241 gcgtggaaag cctgcctgct cataccggtt atcgcatcag cgagctgccg caccgcgcag
     6301 cgcatggcga agtgcgtgcc gcgtacatta tgggcgaaga tccgctacaa actgacgcgg
     6361 agctgtcggc agtacgtaaa gcctttgaag atctggaact ggttatcgtt caggacatct
     6421 ttatgaccaa aaccgcgtcg gcggcggatg ttattttacc gtcaacgtcg tggggcgagc
     6481 atgaaggcgt gtttactgcg gctgaccgtg gcttccagcg tttcttcaaa gcagttgaac
     6541 cgaaatggga tctgaaaacg gactggcaaa tcatcagtga aatcgccacc cgtatgggtt
     6601 atccgatgca ctacaacaac acccaggaga tctgggatga gttgcgtcat ctgtgcccgg
     6661 atttctacgg tgcgacttac gagaaaatgg gcgaactggg cttcattcag tggccttgcc
     6721 gcgatacttc agatgccgat caggggactt cttatctgtt taaagagaag tttgacaccc
     6781 cgaacggtct ggcgcagttc ttcacctgcg actgggtagc gccaatcgac aaactcaccg
     6841 acgagtaccc gatggtactg tcaacggtgc gtgaagttgg tcactactct tgccgttcga
     6901 tgaccggtaa ctgtgcggca ctggcggcgc tggctgatga acctggctac gcacaaatca
     6961 ataccgaaga cgccaaacgt ctgagtattg aagatgaggc attggtttgg gtgcactcgc
     7021 gtaaaggcaa aattatcacc cgtgcgcagg tcagcgatcg tccgaacaaa ggggcgattt
     7081 acatgaccta ccagtggtgg attggtgcct gtaacgagct ggttaccgaa aacttaagcc
     7141 cgattacgaa aacgccggaa tacaaatact gcgccgtgcg cgtcgagccg atcgccgatc
     7201 agcgcgccgc cgagcagtac gtgattgacg agtacaacaa gttgaaaact cgcctgcgcg
     7261 aagcggcact ggcgtaatac agtcctttct acagcctcct ttcggaggct gtttttttat
     7321 ccattcgaac tctttatact ggttactccc tacccaatcg tattatcaaa atgaaaaaaa
     7381 ttatcgcatt gatgttgttt ttgacattct ttgcccacgc caacgactcc gagcctggca
     7441 gccagtattt aaaggcagca gaggccgggg accgacgcgc acaatatttt cttgccgaca
     7501 gctggtttag ctccggcgat ttgagcaaag ccgaatattg ggcacagaaa gccgccgaca
     7561 gcggtgatgc tgatgcctgc gcgctgttgg cgcagatcaa aatcaccaat ccggtcagtc
     7621 tggactatcc acaagcaaaa gttcttgcag agaaagcggc gcaagcgggc agtaaagaag
     7681 gtgaagtaac gctggcgcat attctggtaa atactcaagc gggtaaaccg gattatccaa
     7741 aggcgatttc gctgttagaa aacgcctcgg aagatctgga gaacgactct gccgtcgatg
     7801 cccaaatgct gcttggtttg atttacgcca acggcgtggg cattaaggcc gacgatgaca
     7861 aggcaacctg gtatttcaaa cgcagctctg caatttcccg aaccggttat tccgagtact
     7921 gggcgggaat gatgttctta aacggtgaag aaggttttat cgagaagaac aagcaaaagg
     7981 cgctgcactg gttgaacctg agctgtatgg aagggtttga taccgggtgt gaagagtttg
     8041 aaaaattaac taacggttaa ggtaggcctg ataagacgcg ttaagcgtcg catcaggcgt
     8101 tgatgtcgga tgcagcgtaa acgccttatc agacctacaa aatcacgcta gcgctgtctg
     8161 acgtaggcct gataagacgc accagcgtcg catcaggcat ccacacattg ccgggggata
     8221 tccccggcaa tcttcaatta ttgatccgca gttttatcaa atttgcccag cacttcacgc
     8281 tcataagcca gcgccttttt gcggtcaaat ttgtgttccc acttggcaat caccagcacc
     8341 gccagcgcat tacccaccac gttcagtgca gtacgcgcca tgtcgaggat acggtcaaca
     8401 ccagcaataa acgccagacc ttccagcgga atacctacgc tacccagcgt tgccagcaac
     8461 accacaaacg acacgcccgg cacgccagca atccctttcg aggtcaccat cagcgtcagc
     8521 accagaatga tttcctgcca gatggacagg tcaatgccat acagctgagc gatgaaaata
     8581 gcggcaatac tttgatacag cgtcgaacca tcaaggttaa aagagtaacc ggtcggcacc
     8641 acgaagctgg tgatcgacgc cggtgctccg taggcttcca tcttctcaat aattcgcggc
     8701 agtacgcttt cagagctggc agtggagtac gccagaatca gctcatcttt cagaatacga
     8761 atcaggatcc agacgcttaa cccgcatagg cgcgccacaa ttcccagcac taccagcgcg
     8821 aagaacagaa tggcgaaatg caccagcagc accagtttcg ccagtggcca cagagaagag
     8881 aaaccaaagt tagccaccgt cactgcaatc agcgcaaaca cacccaccgg cgcataacgc
     8941 atcaccatgt gagtcacttt aaacatggtt tcagagatgg agcggaacac ggtcaccagc
     9001 ggttcacgat gcgtcgcggg cagggaggaa agccccagac caaacagcac cgagaagaag
     9061 atgatcggca acatttcgcc tttcgccatc gacgccacaa tattcgtcgg caccagcgac
     9121 aaaatcgtgc ccataatgcc gtgggaactg ctttgtaccg cttccgtagt gctctgatat
     9181 ttcgagatat cgacggtcgc cagctgcgac atatccaccc cggcaccggg ctggaagacg
     9241 ttcgccagag tgatccccaa aatgatggca acggtggtga tcacctcgaa gtagataatg
     9301 gttttcgcgc caatacgccc gagctgtttg gcatcaccaa cacccgcgat acccaccacc
     9361 agcgtggaga tcacaatcgg cacaacaatc attttgatca gatggatgaa gatatcaccc
     9421 gccggagaga gcaaattgac gaccagccag tcgcggctgt cgctatggta gtgcaggtag
     9481 cttcccagga gaatgcccag caccatagca aacagaatct gccaggccag gctgaatttt
     9541 atatttttca tatgaatgac ttcctcaatg gagcgccgtt ccccgttcag aaacgcgaga
     9601 acgtgacata ctgccagggc atgaattgtg ttgcgttggt ttgtagattt ttttaacgct
     9661 tatgatcagt atcatttgcg cggtatgttg cgcaaccctt taacaacaga gcttttcgct
     9721 gcattaatgc ataataacgc gagtttatgg caggattaat gaataaccat ttgttatgga
     9781 aagattttta tttgtttaat ctgcaataat actgattagt gattattttt ggcatatgtt
     9841 cattcgatta ttttttatgc agctcaataa cataatcttc atcaccagat gattaatccg
     9901 atctccgctg caacaatttc gccatattaa tcgactcgac cagctgtgtc cggttgatcc
     9961 ggggtgagtt gagatccatc actttttgcc atagttcgat ggcttgcgcg taattcgcct
    10021 gcataaacgc atcggaagcc agcagcatca gagcggtgat ttcgttagag tccagcgcga
    10081 gggctttgtc gatcattgcg cgagtctggg cggtcatatg ctggctggcc tggtaataaa
    10141 gcaccgtcgc cagcgccgca tacagttcag cgttctcacc acgtagttgc aacgcctgac
    10201 ggtacgccag cagcgaattg ctgtaatcgt tttgccacag atagtactcg cccagtaacg
    10261 cccactgttc gctgttttgt ggattagcgc ggattttatc ctgcaatgcc tgaagctgcg
    10321 cttccgggtt ttgctggctg gcaaactgat gtagcggatc gcgctgacgc tgatactccg
    10381 cacgtaccgc ctgccatttt gggcttaaca gataactacc gatacagagg aataccatca
    10441 atatcgtgag tgttgtcagc attttcaccg gtatttgagg ctgtttcatc gcttcgccct
    10501 cactcgccag aggatcagtg ccatcagcag taacaacacc actggcagcg cccacaacac
    10561 cagcgtctga cccgttaacg gtgggttata gcgaacaaaa tctccgtagc gttcggtcat
    10621 ccagccaatg atttcgactt cacttttccc ctccgccacc atgctgtaaa cctgatggcg
    10681 catactgact gccaccggcg cgttggattc cagtaagttt tgattctggc actgcggaca
    10741 acgtaactgg ctggcaatat ttaacgcctg ttgctgttgt tgcggattgg cgaattgcca
    10801 ggtgtctaca acctgagcgc gcgcaaaaca ggtaaataac agcagtagcg tgagaagccc
    10861 tttatacatc gcgcttcttc ccccgccatc cgcttaacaa tgcgcccgca atcatcaaca
    10921 atcctccccc ccagatccag cgcacaccgc tttgtacata caagcgaaaa gcgtaacgat
    10981 ccgctccagt tttctccccc atgaccgcat accagtcatg gatgccgttc cagcgaattg
    11041 acggttccat catttgctga cgacgagctt cgtaaaaacg ccgctccggc gttaattcac
    11101 caatgcgttg ctgatggtca aacagcgcca ctatcgcttt ttcgctggtg taattgcctt
    11161 tggcttgcag atcgaggcgc tcaaaacgga aggtgtatcc tgccagcgtc acctgctgac
    11221 ccggctgtaa attcaggctg atctcctgac ggctgacgct ggagacaacg atccctgcgg
    11281 caaataacag tatgcccgca tgcgccagca gcgccggaag ctgcgcgcgt ttgccagaga
    11341 caaacgtcgc cagcacaatc accaccaaca tcaacagacc aaacggtaac gtcgcgcggt
    11401 taaaatacgg tgcgccaacg gagagacgtc cccagcccag caggccgtaa atcatcggat
    11461 aaagcgttcc caccagcacg atcagtagca ctgcgcaaaa cagcaacagc gtagcgagga
    11521 ttaacatttc ccgcgataac cccgaaaaac gcaccgccgg gccaccgtcc cgcgctcgcc
    11581 agccatacag agccagagac gcaaggctaa tcagtgcaaa caggctgaac aacggcacag
    11641 cgcgaacgtt atccagagcg aacgcatgaa ccgaaaccag aatgccagaa cggacaatta
    11701 aggtgcccag cagcgacagc atcagagtaa ctatcgccaa caacagcgac cagtggcgaa
    11761 aaatcccccg ctggcgtgtc agggataaac tgtgcagcag cgcagtggca gaaagccagg
    11821 gtaataaaga ggcgttttcc accggatccc agaaccacca gccgccccag ccgagttcgc
    11881 agtaggccca ccaggaaccg aggatgatcc ccgcagttaa tgcactccag ccaggtaacg
    11941 cccagcgcca gcaaattcgg gcgcaggcac catcaaactc gccgcgcagt agactcgcca
    12001 gcgccacgct cgccgctacc atcaaaccgc catagccaag gtaaagcagc ggtggatgaa
    12061 agataagacc gggatgttgc agcatcggat tgagatcgcg gccttcgatt gctggtggaa
    12121 atatccgcac gaagggatcg gaccacagca ctacaaacag tagcagtgcg gcgagcatga
    12181 gagataaaac ggctagcgtc agcggaaata gcggatcggt ttgctgccga tattgccagg
    12241 caaacagcgc gctccaggcg gaaagcagca gcacccaaag cagcagcgaa ccttcatgac
    12301 cgccccacac cgccgccagt ttgagtttcc acgacagcaa gctgtagcta tgttgggcga
    12361 cataaatgac cgagaaatcg ctgatgagaa aacaatacgt cagtacgcca aaggcgagca
    12421 gcaggagcgc gaactgtgcc agaatgccga tgcaagtgag tcgcatcatg gcaggccagc
    12481 gcaaccgcac tcccgcaaag gccgtcaacg gggtcaacac gttgaccccg agacttaaca
    12541 acagcgccag aaaaccgact tcaggaagga atacgtccag aaaccacctc tgttctggct
    12601 tacgccaccg ttaactgccc ggcgtagaga atgaaaaatc gcaacatcaa cacgccgacc
    12661 agactggccc cacacgccgc cagcacggca ggaatgccgg aactgcgatt gacccacggt
    12721 ttgaacaaca ttggcacaat cagccccagc ccggcgacac caagccagaa ccaccacgtc
    12781 cagaaaccgc cgcccaatgc cgccaccagc gcacgcactt taccgtcatc gcccagcgcc
    12841 agaccgacaa aaaacgccgc cagcaggaag atttcacccc ataccacggg gatttccata
    12901 cggtgtacaa actgcgcttc cgtggaatgc gggttactgc gttggcgtat cgccatggcg
    12961 atcagtgcca ccgccgcacc ggacgagatg ccggagaaga ggaacagcac cggcaggatc
    13021 gggttattaa ggaacggata cgatttcagc gccgacagca gaaaaccggt ataagccccc
    13081 aacaacaccg ccagcaccag catcaatgtt tccagtccgc gatgaacggg cgtcagcagg
    13141 ctcagaacct tttgcacgat ccccagcttc ggcaaccagc gttgttgcag ggcaagcaaa
    13201 tcatgttcaa agattttcgc cagccacagc accagcacca ccatgtaaag ctgaaacagc
    13261 atcaccccca tcgacatcac cgaagtaaaa ctgtagtgga acatcagctt ccagaaggtc
    13321 cacggtcttg tcaggtggaa gacgaggctc aacagaccga ggatcaccgc gccaggtccg
    13381 acaatcagcg tggtgcgcag caacgtgctg tctgcaccgc ccgcctgcgg gtagaagcga
    13441 cgtaacagca cggccagcgt caccagaccg gcagaaatac caatcaaaaa caggtagatg
    13501 gcaatcggcc agtcccacac cagcgattca aaatgaaatg cggaagtctg cgtcattggc
    13561 tcacctcccc gtatttaaac ggtacgcggt acagcttcgg tttggttccc agcgccagct
    13621 tgtagcggta agtgggcttc tggcgcagca gttgcgaaat ctcactgttg ggatcgtcca
    13681 gattgccaaa cgtcagcgcc ttcgtcgggc aagcctcaac gcacgcggga agcttaccgg
    13741 cctgcaaatt ggtcttacgg cagaaatcgc atttgtccgc tgtcttcgtg accggatgaa
    13801 taaagcgcac gcggtacgga caggcggcga tgcagtactg acaaccgacg cagagatccg
    13861 gattaacatc gacaatccca ctggcagcat cgcgaaaaga tgcaccggtc gggcagacgt
    13921 caacgcacgg cgcatgatcg cagtgctggc aagacttacg gaagaagcga tatttcacat
    13981 caggaaattc gccctgcggc tcgctacgaa taatcgtcag gcgcgagacg ccttccggca
    14041 ccttgttcac ttcccgacaa gcatccatac aggcggtaca gccgatgcat aacgactcgt
    14101 catgcaccat gccgtaacgc accccattga tattcaacgt tttcgccacc acgcgccctg
    14161 cggtgccgct gacggctgcc agcacgccga cgccggtgag aaactggcga cgagaccagg
    14221 tcatggctgc tccttaagca acggaacaga cgccgggtta aagttcggat tggtgcgctg
    14281 atcgctgtgg caatcgacgc aaatcttaat ccgtcctttg tcacttaacg tctgcatcgt
    14341 atcttgctgt ggatgcaggg aatggcagct ggcgcacgcc actttggtta cgtggacatc
    14401 gtgcggccag aacgcttttt gcaactgttc cggcagatgg caggacatac agacgctgtt
    14461 ctgctctcca accttgtaca tcggctcgtt aaagcgcatc acatctttca ccccttcgcg
    14521 gtgttgcggt gatggctggc cgtgacagtt ggtgcaggtg accggcagtt tgttattcgg
    14581 gttgataacg gaagcatgtt tgccatgcat accttcggta tctggcttat gacagtccag
    14641 acaggcggca tccgggttac gctgctgggt aacttcgtag cgatcatcag atgcctgcgc
    14701 cgcaggggta gcggtaattc cgttcaggct ccacaacagg cccgacgcca gcaccccggc
    14761 agttaataac gaacgtaata cgctcatatt cactccattg tgttttccgc cccttgcgag
    14821 gcggaacggg gttattggct taacagaccg tttttacgtg cctgctcttc ccactgcggg
    14881 atcaccgttt tgatgaagtc ctgcttctcg gccttgattt gttccatgtt caggccaatg
    14941 gcctgctggg ctttctcttt ggtcgagata tccgggatct ggatttcatg ggtgatgcct
    15001 ttagtcgcca gcaggcgcgc cagtttggtg cgtgcatccg ccgctttatc catcgccgta
    15061 ccgagcatcc tcagaccttc ttccggtgcg tgcatatgaa tgccgtggga agcgatcgcc
    15121 agatcccaac gccactgggc atgacggata tcgtcctgaa tcggcttcat ttcggcttcc
    15181 gtcgcacctg cgtccagcgc cgctttcgct tcgaagtgag cgtgaaccag ttgatcttca
    15241 accttgattt tcaggtcgtt aatcgactgc ttacgttccg cgaccacttt ttgcaaggca
    15301 gctttatcct gggtatggca gttcgcacaa gtctgggcga agttatcaaa cggattacca
    15361 attttatggt cggtgtagag tttgccttcg gcgttctgca cttttggcat atggcagtcg
    15421 atacaggtca cgttgttttt accgtgaatg cccgctgtcc aggtttcata ttccgggtgc
    15481 tgcgctttca gcattggcgt tttcgacagg gagttagtcc agtcagagaa ggcaattttg
    15541 tcgtaatact gctccatatt ttcgactttc atgccgtcat cccacgggaa tttaaccgct
    15601 ttgtttttgc cgtcgaagta atactccaca tggcactgac cgcaaaccat cgattgctgg
    15661 tcgaaacgtc cggctttctc aaaaggttta ccaatggctt ccatcgcgcg agccgcatac
    15721 ggacgggaaa gggttaactc cggtttgcct ttggcgaact ctggagaggc ggtgttatgg
    15781 caatcggcac aacctaagtt gttgacgatt tccgggccgc cgcgcgccca tttaccgtgg
    15841 aagtagccat cttcgccgtc tttctggatc agacgcgcca catccgggct tttacaactc
    15901 cagcacgcca tcggtagcgg accatcttca gcgtttttcg gcgcaccggt acgcagggtt
    15961 tcacgcacat cggtcacagc aaaggcatgt ccacgcggct tgttgtaatc gcgcgagaag
    16021 ggataccccg cccacaggat caccagccgt ggatcttccg ccagggcgtc aacacgctct
    16081 gactgttccg aggtggcttt ccaggagaga tattgatcgg gatgctgcgg ggcaaaggtt
    16141 tcattcttcg cttccacagt tacaggtttt gcgggagcag ccgtttgttc agcgtgaaca
    16201 gaagtgaaaa agaaaaaagg aatcaataag ctgaagatac ggcgtgcgtt tatttttatc
    16261 cttgtcatag gggcttcatc cgaattgcgc cattgttgca atggcggttt ttattgtttt
    16321 tcacgacagt aaccgcacct acactgtcat gacattgctc gcccctaagt gtaacaaata
    16381 accacactgt gaatgttgtc tttaatcaat tgtaagtgca tgtaaaatac cactttagag
    16441 ttagtcagta tcttcctctt tttcaacagc atgcataact gcatgttcct caaagaatta
    16501 atcaactttt gttgctgacc ttcaaaaatt accctgccgt ttatttgcac aattctactt
    16561 ttgcgtgatc tgtcgcccaa atactaaaca aaactgccaa tacccctaca tttaacgctt
    16621 atgccacata ttattaacat cctacaagga gaacaaaagc atgagtcaaa ttcacaaaca
    16681 caccattcct gccaacatcg cagaccgttg cctgataaac cctcagcagt acgaggcgat
    16741 gtatcaacaa tctattaacg cacctgatac cttctggggc gaacagggaa aaattctcga
    16801 ctggatcaaa ccgtaccaga aggtgaaaaa cacctccttt gcccccggta atgtgtccat
    16861 taaatggtac gaggacggca cgctgaatct ggcggcaaac tgccttgacc gccatctgca
    16921 agaaaacggc gatcgtaccg ccatcatctg ggaaggcgac gacgccagcc agagcaaaca
    16981 tatcagctat aaagagctgc accgcgacgt ctgccgcttc gccaataccc tgctcaagct
    17041 gggcattaaa aaaggtgatg tggtggcgat ttatatgccg atggtgccgg aagccgcggt
    17101 tgcgatgctg gcctgcgccc gtattggcgc ggtgcattcg gtaattttcg gtggcttctc
    17161 gccggaagcg gttgccgggc gcattatcga ttccaactca cgactggtga tcacttccga
    17221 cgaaggcgtg cgcgccgggc gtagtattcc gctgaagaaa aacgttgatg acgcactaaa
    17281 aaacccgaac gtcaccagcg tagagcatgt ggtggtactg aagcgtactg gcgggaaaat
    17341 tgactggcag gaagggcgcg acctgtggtg gcacgaccag gttgagcaag ccagcgatca
    17401 gcaccaggcg gaagagatga acgccgaaga tccgctgttt attctctata cctccggttc
    17461 taccggaaaa ccaaaaggcg tactgcacac taccggcggt tatctggtgt acgcggcgct
    17521 gacctttaaa tatgtctttg attatcatcc gggcgatatc tactggtgca ccgccgatgt
    17581 gggctgggtg accggacaca gttatttgct gtacggcccg ctggcctgcg gcgcgaccac
    17641 gctgatgttt gaaggcgtac cgaactggcc gacgcctgcc cgtatggcac aggtggtgga
    17701 caagcatcag gtcaatattc tctataccgc gcccacggcg attcgcgcgc tgatggcgga
    17761 aggcgataaa gcgatcgaag gcaccgaccg ttcgtcgctg cgcattctcg gttccgtggg
    17821 cgagccaatt aacccggaag cgtgggagtg gtactggaaa aaaatcggca acgagaaatg
    17881 tccggtggtc gatacctggt ggcagaccga aaccggcggt ttcatgatca ccccgctgcc
    17941 tggcgctacc gagctgaaag ccggttcggc aacacgtccg ttcttcggcg tgcaaccggc
    18001 gctggtcgat aacgaaggta acccgctgga aggggctacc gaaggtagcc tggtgatcac
    18061 cgactcctgg ccgggtcagg cgcgtacgct gtttggcgat cacgaacgtt ttgagcagac
    18121 ctatttttcc accttcaaaa atatgtattt cagcggcgac ggcgcgcgtc gtgatgaaga
    18181 tagctattac tggatcaccg ggcgtgtgga cgatgtgctg aacgtctccg gtcaccgtct
    18241 gggaacggcg gagattgagt cggcgctggt ggcgcatccg aaaatcgccg aagccgctgt
    18301 cgtcggtatt ccgcacaata ttaaaggtca ggcgatctac gcctacgtca cgcttaatca
    18361 cggggaggaa ccgtcaccag aactgtacgc agaagtccgc aactgggtgc gtaaagagat
    18421 tggcccgctg gcgacgccag acgtgctgca ctggaccgac tccctgccta aaacccgctc
    18481 cggcaaaatt atgcgccgta ttctgcgcaa aattgcggcg ggcgatacca gcaacctggg
    18541 cgatacctcg acgcttgccg atcctggcgt agtcgagaag ctgcttgaag agaagcaggc
    18601 tatcgcgatg ccatcgtaac ccacaattgc cggatgcgag tcggtaacgg tttgtaggcc
    18661 tgataagacg cgacagcgtc gcatcaggca ttgattgccg gatgcggcgt ataacgcctt
    18721 atccggccta cattcggcaa gggttacccg agcgttaacc ttctcccata agggagcggg
    18781 aattaaaaca atccctacat tacctctgga gaatctgtga tgaatggcac tatttatcag
    18841 cggatagaag acaatgcgca tttcagggag ttagtcgaaa aacggcaacg gtttgccacc
    18901 atcctgtcga ttattatgct ggcagtttat atcggcttta ttttactgat cgccttcgcg
    18961 cccggctggc tgggcacccc gctgaatccg aacaccagcg tcacacgcgg tattccagtc
    19021 ggtgttgggg taatagtgat ctcctttgtt ctcactggta tctacatctg gcgggcgaac
    19081 ggcgaattcg accgtcttaa taatgaagtc ctgcatgagg tacaagcatc atgaaaagag
    19141 ttctgacggc gcttgccgcc acactccctt tcgcagctaa cgccgcggat gctattagcg
    19201 gggccgtaga gcgccagcca acgaactggc aggcgattat tatgttcctg attttcgtcg
    19261 tgtttacgct cggcattacc tactgggcgt caaaacgcgt acgttcgcgt aatgactatt
    19321 acaccgcagg cggcaatatc accggttttc agaacgggct ggcgattgcc ggtgactata
    19381 tgtccgccgc ctcatttttg gggatctccg cactggtgtt tacctccggc tatgacggct
    19441 taatttactc gctgggcttc ctggtgggct ggccgatcat tttgttcctg attgccgaac
    19501 gtctgcgtaa cctggggcgt tacacctttg ccgatgtagc ctcttatcgt ctgaaacaag
    19561 ggccgattcg tattctttcg gcctgtggtt ctctggtggt ggtggcgctt taccttatcg
    19621 cccagatggt gggtgcaggt aaactgatcg agctgctgtt tggccttaac tatcacattg
    19681 cagtggtgct ggtcggcgtg ctgatgatga tgtacgtcct gttcggcggc atgctggcga
    19741 ccacctgggt acagattatc aaagctgtgc tgttgctgtt cggtgccagc tttatggcct
    19801 ttatggtgat gaaacacgtc ggctttagct tcaacaatct gttcagcgaa gcgatggcgg
    19861 tacacccgaa aggtgtcgac atcatgaaac cgggcggact ggtgaaagat ccgatctccg
    19921 cactctctct ggggctagga ctgatgtttg gtacggctgg cttgccgcac attctgatgc
    19981 gcttctttac ggtcagcgat gcccgcgaag cgcgtaaaag cgtgttctac gctaccggat
    20041 ttatgggcta cttctatatc ctgaccttta ttatcggctt cggcgcgatc atgctggtcg
    20101 gtgcgaatcc ggaatataaa gacgcggcgg gccatctgat tggtggtaac aacatggcag
    20161 cagttcacct ggccaatgcg gtgggcggca acctgttcct cggctttatt tcagcggttg
    20221 ctttcgccac tatcctcgcg gtggttgcgg gtctgacgct ggcgggcgca tcagcggttt
    20281 cgcatgacct gtacgctaac gtcttcaaaa aaggcgcgac cgaacgtgaa gagctgcggg
    20341 tatcgaaaat caccgtcctg attttgggtg tgattgcgat tatcctcggc gtgctgtttg
    20401 agaatcagaa catcgccttt atggtggggc tggcgtttgc catcgcggcg agctgtaact
    20461 tcccgatcat tctgctttct atgtactggt cgaaactgac cacgcgtggc gcgatgatgg
    20521 gtggctggct ggggctgatt accgcagtgg tactgatgat cctcggcccg acgatttggg
    20581 tacagatcct cggtcacgaa aaagccatct tcccgtatga atacccggcg ctgttctcta
    20641 tcaccgtggc attcctcggc atctggttct tctcggcaac cgataactca gcggaaggcg
    20701 cgcgtgagcg tgaactgttc cgcgcgcagt ttatccgctc ccagaccggc tttggcgttg
    20761 agcaaggccg cgcgcattaa tctctcccct tccccggtcg tctgaccggg gaatactctt
    20821 cctctcccaa atgcatcacc ttttcccata atattaaaca aataaactca ttaaaaaaaa
    20881 atgagcgatt tttgacagtc gtagaaaatg ataatgcaga gaatatgcct tttctttctt
    20941 gttaattata aggatatttt atgcgctata atggtttaaa
//
LOCUS       DAZFBR010000083        20710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFBR010000083 DAZFBR010000000
VERSION     DAZFBR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.083"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..1161)
                     /gene="espX2"
                     /locus_tag="ACHJZ2_003361"
     CDS             complement(<1..1161)
                     /gene="espX2"
                     /locus_tag="ACHJZ2_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO4569414.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRDEKGITVR
                     IDNINGKKNNSNSSDVFIPYVNTFPDLKNKILRMKIELTEGSGFSKSLSDSQIEMHIL
                     RTVNSLNVGEKLNDDNLADHSIFTNEFSVIIPPSYYDATSAVNANNIVREKLFESDSK
                     VKDIVDDMSNHDVESEKDIFVIGGMIEKLNSLADESFNDSTDNIQTVKDLLTQLTDGM
                     ELFALRDIVAFPSTIIAKLIKSPLNSDHELVMRALDTYLCYFRNKNLNNNAEIINFFH
                     ALFLKRPELMVAENYRFIQFIDLLFENGNVEEKNLAFDLYHNYLSLSEIKQFV"
     gene            complement(1769..3136)
                     /gene="rhlE"
                     /locus_tag="ACHJZ2_003362"
     CDS             complement(1769..3136)
                     /gene="rhlE"
                     /locus_tag="ACHJZ2_003362"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO4569415.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            3365..4036
                     /gene="cecR"
                     /locus_tag="ACHJZ2_003363"
     CDS             3365..4036
                     /gene="cecR"
                     /locus_tag="ACHJZ2_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO4569416.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            4039..5034
                     /gene="hlyD"
                     /locus_tag="ACHJZ2_003364"
     CDS             4039..5034
                     /gene="hlyD"
                     /locus_tag="ACHJZ2_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO4569417.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            5027..6763
                     /gene="ybhF"
                     /locus_tag="ACHJZ2_003365"
     CDS             5027..6763
                     /gene="ybhF"
                     /locus_tag="ACHJZ2_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4569418.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            6756..7889
                     /gene="ybhS"
                     /locus_tag="ACHJZ2_003366"
     CDS             6756..7889
                     /gene="ybhS"
                     /locus_tag="ACHJZ2_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4569419.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            7900..9006
                     /gene="ybhR"
                     /locus_tag="ACHJZ2_003367"
     CDS             7900..9006
                     /gene="ybhR"
                     /locus_tag="ACHJZ2_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4569420.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(8968..9378)
                     /gene="ybhQ"
                     /locus_tag="ACHJZ2_003368"
     CDS             complement(8968..9378)
                     /gene="ybhQ"
                     /locus_tag="ACHJZ2_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO4569421.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            9511..10272
                     /gene="ybhP"
                     /locus_tag="ACHJZ2_003369"
     CDS             9511..10272
                     /gene="ybhP"
                     /locus_tag="ACHJZ2_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4569422.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            10269..11510
                     /gene="clsB"
                     /locus_tag="ACHJZ2_003370"
     CDS             10269..11510
                     /gene="clsB"
                     /locus_tag="ACHJZ2_003370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO4569423.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            11510..12466
                     /gene="ybhN"
                     /locus_tag="ACHJZ2_003371"
     CDS             11510..12466
                     /gene="ybhN"
                     /locus_tag="ACHJZ2_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO4569424.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(12502..13215)
                     /gene="ybhM"
                     /locus_tag="ACHJZ2_003372"
     CDS             complement(12502..13215)
                     /gene="ybhM"
                     /locus_tag="ACHJZ2_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4569425.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIVVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIILHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(13423..14127)
                     /gene="ybhL"
                     /locus_tag="ACHJZ2_003373"
     CDS             complement(13423..14127)
                     /gene="ybhL"
                     /locus_tag="ACHJZ2_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4569426.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(14263..14715)
                     /gene="moaE"
                     /locus_tag="ACHJZ2_003374"
     CDS             complement(14263..14715)
                     /gene="moaE"
                     /locus_tag="ACHJZ2_003374"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO4569427.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFIGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(14717..14962)
                     /gene="moaD"
                     /locus_tag="ACHJZ2_003375"
     CDS             complement(14717..14962)
                     /gene="moaD"
                     /locus_tag="ACHJZ2_003375"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO4569428.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(14955..15440)
                     /gene="moaC"
                     /locus_tag="ACHJZ2_003376"
     CDS             complement(14955..15440)
                     /gene="moaC"
                     /locus_tag="ACHJZ2_003376"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO4569429.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(15443..15955)
                     /gene="moaB"
                     /locus_tag="ACHJZ2_003377"
     CDS             complement(15443..15955)
                     /gene="moaB"
                     /locus_tag="ACHJZ2_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO4569430.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(15977..16966)
                     /gene="moaA"
                     /locus_tag="ACHJZ2_003378"
     CDS             complement(15977..16966)
                     /gene="moaA"
                     /locus_tag="ACHJZ2_003378"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO4569431.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(16954..17098)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            17363..18271
                     /gene="yvcK"
                     /locus_tag="ACHJZ2_003379"
     CDS             17363..18271
                     /gene="yvcK"
                     /locus_tag="ACHJZ2_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO4569432.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(18462..20483)
                     /gene="uvrB"
                     /locus_tag="ACHJZ2_003380"
     CDS             complement(18462..20483)
                     /gene="uvrB"
                     /locus_tag="ACHJZ2_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO4569433.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         5026 a         5359 c         5255 g         5070 t
ORIGIN      
        1 aacgaattgt tttatttcag ataatgaaag ataattgtga taaaggtcaa atgctaagtt
       61 tttctcttca acatttccgt tttcaaaaag caaatcaata aattgaataa atctataatt
      121 ttcagcaacc atcaactctg gtctctttaa aaagagggca tggaaaaagt taattatttc
      181 cgcattgtta ttcagatttt tatttctgaa gtagcacaga tacgtatcca gtgcgcgcat
      241 aacaagttca tgatcgctat taagtgggga ttttattaat tttgctatta tggttgatgg
      301 gaatgccact atgtcccgca gcgcgaagag ctccatgcca tcagttaatt gcgttaacag
      361 atcttttact gtttgtatgt tatcagtact atcattgaaa gattcgtctg ccagagaatt
      421 cagtttttct atcatccccc cgattacaaa tatatctttt tcgctctcca cgtcatgatt
      481 agacatatca tcgacgatat ctttaacttt actatcactt tcaaatagtt tttctctcac
      541 aatattatta gcgtttactg cagaagttgc atcataatag gatggcggaa taataacaga
      601 gaattcgtta gtgaatatag aatgatctgc aagattatca tcattcaatt tctcgccaac
      661 atttagcgaa ttaacagtcc tcagaatatg catctcaatc tgactgtcag aaagactttt
      721 agaaaaacca ctaccttcag tcagttcaat tttcatcctg agtattttat ttttgaggtc
      781 gggaaatgta tttacataag gaataaaaac atctgatgaa ttagagttgt ttttctttcc
      841 attaatatta tctattctaa cggtgatccc tttctcatca cgaggaattg catcattcat
      901 atttatagag atcacatttt catgtctgga ttgcccggtt ttaatcgtca tattgatatc
      961 atttgcgctg atctccgaaa tctcactgac gaatttttta aatgcattat agcgctggtg
     1021 gccagcatta aatttgccaa atgaaacata atatgcgaaa tgttcaagga aaccatgtaa
     1081 ttccagtaaa ttaaccgggt cagaaccctg caccatattt aataacatcg tcgcataacc
     1141 attgcatttt gaacaatcca tacatcgctc ctttgcactt gaatgatctg cgaatcataa
     1201 gcctggcaaa atggggagtc ttacagaaat cgctcatttt taacttttcg tactgcaaat
     1261 tcaggatcaa cctggttatg aaagtttgct gctgtacggc tctaaacaga ctggcaatca
     1321 atctttctat caataagctg aaatgagcga tttttgatag tttattagaa ctactaaaga
     1381 tatacggtaa tgaaacgact gcacacttta ttttgcttag ggagatagtc ggatacacta
     1441 tagacaatga aaataataaa atcagggaca gaatcaagct aatactgaat cacatatctc
     1501 ataataattt accagatgat gaacaaactc tttatgcaat agcatgttta ttccagattt
     1561 tcccagaatt tgtttatgaa tatcaaccaa tgttattaac aataattcaa attgcaagga
     1621 gaaataattt cattgccatt ttagatacac tagatagatc ataaagcaaa tttattaaaa
     1681 caataaaatc agacatgtca tgcatgtctg accgtgttac ctgaataatg ggcaaaagcc
     1741 tgtgatactc ttttgccctt acatggtatt actgcgcagc ggcaggttta cgcggacggc
     1801 gacggcgttg ttgttcgcct gccggtttgg catcgccaag acgacgagat ggtttttctg
     1861 caggcttcgc gctagctgat ttcgcaccac cttctccacg gcgtggttgc tgttgaccgc
     1921 gaccaccacc accttgccca cgaccgccgc cgccacgttg ctggcgaccg ttctggatcg
     1981 gttcggcttt gattgacggg tccggctcat aacccggaat cgcaatgcgc gggatctctt
     2041 ttttcagcag tttttcgata tcacgcagca gtttgtgttc atcaacacac accagcgaca
     2101 atgcttcgcc agttgccgcc gcacggccag tacgcccgat acggtggaca taatcttccg
     2161 gtacgtttgg cagttcatag ttgacgacgt gcggcaactc ttcgatatcc agaccgcgcg
     2221 cagcgatgtc agttgccacc agtacacgaa tatcgcccga tttaaaatcg gccagcgcac
     2281 gggtacgcgc accttgcgat ttattaccgt ggatcgccgc actacggatg ccatctttat
     2341 taagctgttc agcgagatgg ttagcgccat gtttggtacg ggtaaacacc agcacctgct
     2401 gccagttccc tttcccaatc atgtgcgaca gcaattcgcg cttgcgtttc ttatcaacaa
     2461 agtgaacatg ctgggtcacc tgatcagacg cggtattgcg gcgtgccact tctatttcca
     2521 gcgggttgtg caacagtttt tccgccaggg ctttaatatc gtcagagaag gtcgcggaga
     2581 acaacaggtt ctggcgcttc gcaggtagtt ttgttaacac gcgacggata tcgtggataa
     2641 agcccatgtc gagcatgcgg tcagcttcat cgaggacgag gatttcaacc tgatccagct
     2701 tcactgcatt ctgatgttcc aggtccagca aacgtcccgg cgttgccacc agcacatcaa
     2761 cgccgccgcg caacttcatc atttgtgggt taatgctgac accgccaaac accaccagcg
     2821 aacgaatgtt caggtatttg ctgtaatcac ggacgttttc gccaatctgc gccgccagtt
     2881 cacgggtcgg ggtaagaatg agcgcacgta ccggacgacg ccctttggcg tgcggctggc
     2941 gagtgatcag gtgttgcaac agcggcagcg taaagcccgc tgttttgccg gtgccggtct
     3001 gggcgctagc catcaggtcg cggccttcca gcaccgcagg gatcgcctgc tgctgaatag
     3061 gggtgggttc acggtaaccc tgctcggcaa cggcgcgcag gatatcaggg cttaaaccca
     3121 aagaatcgaa agacataact actccgaacc gccccgaccg ttaccggtgt agttttcagg
     3181 gagatactga aaaggaaaat gacaaaaacc acaatgtcat gagggagcgg agtgtagcag
     3241 tttttgtgac gcagcgcata aattatccct gtgactggcg tagctaaaat tcttagccat
     3301 actggacaac tcccatatgg agtcataatc ttaatcaatc atttgattaa gaatggactg
     3361 tgcgatgaat aatcctgcca tgacaatcaa gggtgaacag gcgaaaaaac agctgattgc
     3421 tgccgcactg gcgcagtttg gtgaatatgg aatgaacgcc accacccgcg agatagccgc
     3481 ccaggccggg cagaatatcg ctgccatcac ctactacttc ggttcgaaag aagatttgta
     3541 cctcgcctgc gcccagtgga ttgccgattt tattggcgag cagttccgtc cgcatgccga
     3601 ggaagccgaa cgcttgttcg cccaaccaca gcctgatcgg gctgccatcc gtgaactgat
     3661 ccttcgcgcc tgcaggaaca tgattaagct gctcacccag gatgacaccg tcaacctcag
     3721 caagtttatc tcccgtgagc agctctctcc cacggcagcc taccacctgg tgcacgaaca
     3781 ggtgattagt ccgctacaca gccacctgac acgcctgatt gccgcctgga ccggctgcga
     3841 cgccaatgac acccgcatga tccttcatac ccatgcgttg attggtgaga ttctggcgtt
     3901 ccgtcttggc aaagaaacaa ttctgttacg taccggctgg accgcgttcg atgaagaaaa
     3961 gaccgaactg atcaaccaga cggtgacctg tcatatcgac ctgattttgc aaggattatc
     4021 gcaaaggagt ttgtagtgat gaaaaaacct gtcgtgatcg gactggcggt agtggtactt
     4081 gccgccgtgg ttgccggagg ctactggtgg tatcaaagcc gccaggataa cggcctgacg
     4141 ctgtatggca acgtggatat tcgtacggtg aatcttagtt tccgtgttgg ggggcgcgtt
     4201 gaatcgctgg cggtggacga aggtgatgct atcaaagcgg gccaggtgct gggcgaactg
     4261 gatcacaagc cgtatgagat tgccctgatg caggcgaaag cgggcgtctc ggtggcacag
     4321 gcgcagtatg acctgatgct tgccgggtat cgcgatgaag aaatcgctca ggccgccgca
     4381 gcggtgaaac aggcgcaagc cgcctatgac tatgcgcaga acttctataa ccgccagcaa
     4441 ggactgtgga aaagccgcac catttcggca aatgacctgg aaaatgcccg ctcctcgcgc
     4501 gaccaggcac aggcaacgct gaaatcagca caggataaat tgcgtcagta ccgttccggt
     4561 aaccgtgaac aggacatcgc tcaggcgaaa gccagcctcg aacaggcgca ggcgcaactg
     4621 gcgcaggcgg agctgaattt acaggactca acgttgatag caccgtctga tggcacgctg
     4681 ttaacgcgcg cggtggagcc aggcacggtt ctcaatgaag gtggcacggt gtttaccgtt
     4741 tcactaacgc gtccggtgtg ggtgcgtgct tatgttgatg aacggaatct tgaccaggcc
     4801 cagccggggc gcaaagtgct gctttatacc gatggtcgcc cggacaagcc ctatcacggg
     4861 cagattggtt tcgtttcgcc gactgctgaa tttaccccga aaaccgtcga aacaccggat
     4921 ctgcgtaccg acctcgtcta tcgcctgcgt attgtggtga ccgacgccga tgatgcgtta
     4981 cgccagggaa tgccagtgac ggtacaattc ggtgacgagg caggacatga atgatgccgt
     5041 tatcacgctg aacggcctgg aaaaacgctt tccgggcatg gacaagcccg ccgtcgcgcc
     5101 gctcgattgc accattcatg ccggttatgt gacggggctg gtagggccag acggtgcggg
     5161 taaaaccacg ctgatgcgga tgctggcggg attactgaaa cccgacagcg gcaatgccac
     5221 ggtgattggc tttgatccga tcaaaaacga cggcgcgctg cacgccgtgc tcggctatat
     5281 gccgcagaaa ttcggcctgt atgaagatct gacggtgatg gagaacctca atctgtacgc
     5341 ggatttgcgc agcgtcaccg gcgaggcacg gaagcagact tttgctcgcc tgctggagtt
     5401 tacgtctctt ggcccgttta ccggacgcct ggcgggcaag ctctccggtg ggatgaaaca
     5461 aaaactcggc ctggcctgta ccctggtggg cgaaccgaaa gtgttgctgc tcgatgaacc
     5521 cggcgtcggc gttgacccta tctcacggcg cgaactgtgg cagatggtgc atgagctggc
     5581 gggcgaaggg atgttaatcc tctggagtac ctcgtatctc gacgaagccg agcagtgccg
     5641 tgacgtgtta ctgatgaacg aaggcgagtt gctgtatcag ggagaaccaa aagccctgac
     5701 acaaaccatg gccggacgca gctttctgat gaccagtcca cacgagggca accgcaaact
     5761 gttgcaacgc gccttgaaac tgccgcaggt cagcgacggc atgattcagg ggaaatcggt
     5821 acgtctgatc ctcaaaaaag aggccacacc agacgatatt cgccatgccg acgggatgcc
     5881 ggaaatcaac atcaacgaaa ctacgccgcg ttttgaagat gcgtttattg atttgctggg
     5941 cggtgccgga acctcagaat cgccgctggg tgcaatattg catacggtag aaggcacacc
     6001 cggcgagacg gtgatcgaag cgaaagaact gaccaagaaa tttggtgatt ttgccgccac
     6061 cgatcacgtc aactttgccg ttaaacgcgg ggagattttt ggtttgctgg ggccaaacgg
     6121 cgcgggcaaa tcgaccacct ttaagatgat gtgcggtttg ctggtgccga cttccggcca
     6181 ggcgctggtg ctggggatgg atctgaaaga gagttccggt aaagcgcgcc agcatctcgg
     6241 ctatatggcg caaaaatttt cgctctacgg caacctgacg gtcgaacaga atttacgctt
     6301 tttctctggt gtgtatggct tacgcggtcg ggcgcagaac gaaaaaatct cccgtatgag
     6361 cgaggcattt ggcctgaaaa gtattgcctc acacgccacc gatgaactgc cattaggttt
     6421 taaacagcgg ctggcgctgg cctgttcgct gatgcatgaa ccggatattc tgtttctcga
     6481 cgaaccgact tccggcgttg accccctcac ccgccgtgaa ttctggctgc acatcaacag
     6541 catggtagag aaaggcgtca cggtgatggt caccacccac tttatggatg aagcggaata
     6601 ttgcgaccgc atcggcctgg tgtaccgcgg gaaattaatc gccagcggca cgccggacga
     6661 tttgaaagca cagtcggcta acgatgagca acccgatccc accatggagc aagcctttat
     6721 tcagttgatc cacgactggg ataaggagca tagcaatgag taacccgatc ctttcctggc
     6781 gtcgcgtgcg ggcgctgtgc gtcaaagaga cgcggcagat cgttcgcgat ccgagtagct
     6841 ggctgattgc ggtagtgatc ccgctgttgc tgctgtttat ttttggttat ggcattaacc
     6901 tcgactccag caagctgcgg gtcgggattt tactggaaca gcgtagcgaa gcagcgctgg
     6961 atttcaccca caccatgacc ggttcgccct acatcgacgc caccatcagc gataaccgtc
     7021 aggaactgat cgccaaaatg caggcgggga aaattcgcgg tctggtggtt attcccgtgg
     7081 atttcgccga acagatggag cgcgccaacg ctaccgcacc gattcaggtg atcaccgacg
     7141 gcagtgagcc gaataccgct aactttgtgc agggatatgt cgaagggatc tggcagatct
     7201 ggcaaatgca gcgggcggag gataacgggc agacctttga gccgcttatt gatgtacaaa
     7261 cccgctactg gtttaacccg gcggcgatta gccagcactt tattatcccc ggtgcggtga
     7321 ccatcatcat gacggtcatc ggcgcgattc tcacctcgct ggtggtggcg cgagaatggg
     7381 aacgcggcac tatggaggct ctgctctcta cggagattac ccgcacggaa ctgctgctgt
     7441 gtaagctgat cccttattac tttctcggga tgctggcgat gttgctgtgt atgctggtgt
     7501 cagtgtttat tctcggcgtg ccgtatcgcg gttcgctgct gattctgttt tttatctcca
     7561 gcctgttttt actcagtacc ctggggatgg ggctgctgat ttccacgatt acccgcaacc
     7621 agttcaatgc cgctcaggtc gccctgaacg ccgcgttcct gccgtcgatt atgctttccg
     7681 gctttatttt tcagatcgac agtatgcccg cggtgatccg cgcggtgacg tacattattc
     7741 ccgcacgtta tttcgtcagc accctgcaaa gcctgttcct cgccgggaat attccagtgg
     7801 tgctggtggt gaacgtactg tttttgatcg cttcggcggt gatgtttatc ggcctgacgt
     7861 ggctgaaaac caaacgtcgg ctggattagg gagaagagca tgtttcatcg cttatggacg
     7921 ttaatccgca aagagttgca gtcgttgctg cgcgaaccac aaacccgcgc gattctgatt
     7981 ttaccggtgc tgattcaggt gattctgttc ccgttcgccg ccacgctgga agtgaccaac
     8041 gccaccatcg ccatctacga tgaagataac ggcgagcatt cggtggagct gacccaacgt
     8101 tttgcccgcg ccagcgcctt tacacatgtg ctgctgctga aaagcccaca ggagatccgc
     8161 ccgaccatcg acacacaaaa ggcgttacta ctggtgcgtt tcccggctga cttctcgcgc
     8221 aaactggata ccttccagac cgcgccgttg cagttgatcc tcgacgggcg taactccaac
     8281 agtgcgcaaa ttgccgccaa ctacctgcaa cagatcgtca aaaattatca gcaggagctg
     8341 ctggaaggaa aaccgaaacc taacaacagc gagctggtgg tacgcaactg gtataacccg
     8401 aatctcgact acaaatggtt tgtggtgccg tcgctgatcg ccatgatcac cactattggc
     8461 gtaatgatcg tcacttcact ttccgtcgcc cgcgaacgtg aacaaggtac gctcgatcag
     8521 ctactggttt cgccgctcac cacctggcag atcttcatcg gcaaagccgt accggcgtta
     8581 attgtcgcca ccttccaggc caccattgtg ctggcgattg gtatctgggc gtatcaaatc
     8641 cccttcgccg gatcgctggc gctgttctac tttacgatgg tgatttatgg tttatcgctg
     8701 gtgggattcg gtctgttgat ttcatcactc tgttcaacac aacagcaggc gtttatcggc
     8761 gtgtttgtct ttatgatgcc cgccattctc ctttccggtt acgtttcgcc ggtggaaaac
     8821 atgccagtat ggctgcaaaa cctgacgtgg attaatccta ttcgccactt tacggacatt
     8881 accaagcaga tttatttgaa ggatgcgagt ctggatattg tgtggaatag tttgtggccg
     8941 ctactggtga taacggccac gacagggtca gcggcgtacg cgatgtttag acgtaaggtg
     9001 atgtaacttc ttatctttcg ccagcaaaga cactaccgcc gggccggcaa ggattgccag
     9061 ccctgcaatt gccagcaaaa agttgttttc cagtacgcgg gacaacagcc acagcacaat
     9121 cagcgctgcg ccaaaatacc aggtcgtggt cagctcttcc agcacgtctg ccacctcacg
     9181 ccagcgttgc tcggggtggc gctgaaacac cagcatcatc actaccgtga cacaatacag
     9241 tgcgcataat cccacgccga cgtgaaccag agtcttactc atccagccca ccaccagcag
     9301 cgccactacc agtaaatgca acataatctg ccagcaactt agacccgttg cgacacgaac
     9361 acgttgttgc cacttcatgg cttctctcct gaagaattta tctctactta ctcagagtac
     9421 cgaactttac ggaaaattcc gtaagaccgc acctttttgt gacgccgact acactatttc
     9481 aatgagataa gaggcaaaga caggaaaatt atgcccgatc aaacacaaca attttcgttc
     9541 aaggtgctca ccatcaatat tcacaaaggc tttaccgcgt ttaaccgacg cttcattttg
     9601 ccggaacttc gcgatgccgt gcgtaccgtc agcgccgata ttgtttgcct gcaggaagtg
     9661 atgggcgcgc acgaagttca tccgctgcat gtggaaaact ggcccgatac ctcgcactac
     9721 gagtttctcg ccgacactat gtggagcgat tttgcctacg gtcgcaatgc cgtatacccg
     9781 gaagggcatc acggcaacgc cgtactgtcg cgttatccca ttgaacatta tgagaatcgc
     9841 gatgtttcgg tcgatggtgc ggaaaagcgc ggcgtgctct actgccgcat tgtgccgccg
     9901 atgaccggaa aagcgattca tgtgatgtgc gtacatctgg gcctgcgtga ggcgcaccgt
     9961 caggcgcagc ttgcgatgct cgccgaatgg gtgaatgagc taccggacgg cgaaccggta
    10021 ttggtggcgg gtgatttcaa tgactggcgg caaaaagcta atcatccgtt aaaagtgcag
    10081 gccggactgg atgagatttt tacccgcgcc cacggacgcc cggcgcgcac gtttccggtg
    10141 caatttcctc tactacgact ggacaggatc tacgtcaaaa atgccagcgc cagcgcgcca
    10201 accgcgttgc cgctgcggac atggcgacac ctttctgatc atgccccttt aagtgcggag
    10261 attcatttat gaaatgtagc tggcgcgaag gcaataagat ccagttgctg gaaaacggcg
    10321 agcaatatta tcccgcggtg tttaaggcga ttggcgaggc acaagaacgc atcattcttg
    10381 aaacgtttat ctggtttgag gatgacgtcg gcaaacaact gcatgcggca ctactggcag
    10441 cagcgcaacg cggggttaaa gcggaagtct tgctggatgg ctacggttcg ccggatctca
    10501 gcgatgagtt tgtcaatgaa ctgacggcag ctggcgtagt gttccgctac tacgatcccc
    10561 gccctcgcct ttttggtatg cgcaccaatg tgtttcgccg gatgcatcgc aaaattgtgg
    10621 taatcgacgc gcgtatcgcc tttattggcg ggctgaatta ctccgccgag catatgtcca
    10681 gctacggtcc agaggctaaa caggattacg cggtacgcct tgaagggccg attgtcgaag
    10741 atattctcca gtttgagctg gaaaacctgc ctggacagag cgcggcacga cgctggtggc
    10801 gacgtcatca caaagcggaa gagaaccgcc agccgggaga agcgcaggta ttgctggtct
    10861 ggcgcgataa cgaagaacat cgcgatgata ttgaacgtca ctatctgaaa atgctcactc
    10921 aggcgcggcg agaagtcatt atcgccaacg cctacttctt ccccggctat cgatttttac
    10981 acgccttgcg taaagcggca cggcgcgggg tgcggatcaa actgatcatt cagggcgaac
    11041 cggatatgcc gattgtcaga gtcggtgcgc gcttgctgta taactatctg gttaaaggcg
    11101 gcgttcaggt gtttgagtac cgccgccgcc cgctccacgg caaagtggca ttgatggacg
    11161 atcactgggc gacagtaggg tccagtaatc tcgatccgct cagtttgtca ctgaatctcg
    11221 aagcaaatgt catcatccac gatcgtcatt ttaaccagac gctgcgcgat aatctgaacg
    11281 gcattattgc cgcagattgt cagcaggtcg atgagaccat gctgccgaaa cgcacctggt
    11341 ggaacctaac caaaagcgtg ctggcgttcc actttttacg ccacttcccg gcgctggttg
    11401 gctggcttcc ggcacacacg ccacgtctgg cgcaggttga tcctcccgca caaccgacaa
    11461 tggaaacgca ggatcgggta gaaactgaaa acacgggggt aaaaccctga tgagtaaatc
    11521 acacccgcgc tggcgcttag caaagaagat cctcacctgg ctgtttttta tcgcggtgat
    11581 tgtgttgctg gtggtctacg ccaaaaaagt ggactgggaa gaggtctgga aggtcatccg
    11641 cgactacaat cgcgttgcgc tgcttagtgc ggtcgggctg gtggtcgtca gctatctgat
    11701 ttacggctgc tatgacctgc tcgcccgctt ttactgcggt cacaaactgg cgaagcgcca
    11761 ggtgatgctg gtgtcgttta tctgctacgc cttcaacctg acgctcagta cctgggtcgg
    11821 cggcattggt atgcgctatc gtttgtactc ccgtctggga ttgccgggca gcactattac
    11881 gcggattttc tcgctcagta ttaccaccaa ctggctgggc tatattttac tggcagggat
    11941 tatctttacc gcaggcgtgg tggagttgcc ggaccactgg tatgtcgatc aaactacgct
    12001 gcgtattctc ggcattggct tactgatgat tatcgcggtt tatttgtggt tttgcgcttt
    12061 cgcgaagcac cgccatatga ccatcaaagg gcaaaagctg gtgctgcctt cgtggaaatt
    12121 cgccctcgcc cagatgctga tttccagcgt taactggatg gtcatggggg cgattatctg
    12181 gctgttactt ggtcaaagcg tgaactattt ctttgtactg ggcgtgttgc tggttagcag
    12241 tattgccggc gtcatcgtgc atgttccggc ggggattggt gtgctggaag cggtgtttat
    12301 cgcgctactg gctggggagc atacatccaa aggcacaatt atcgccgccc tactcgctta
    12361 ccgtgtgctg tattacttta tcccgctgct gctggcgctg atctgttacc tgttgctgga
    12421 aagccaggcg aagaagctac gggcgaaaaa tgaagcggcg atgtgaagtt tagttaagtt
    12481 ctttagtatg tgcatttacg actaatgaaa aaaacgcgta tgcctttgcc agacaagcgt
    12541 tatagctatc gcggcaagta tcccaaagaa gccgataaaa tagtaataga gtgttaatgc
    12601 acctgtaaaa gcgcaacgaa ccggactcca caactcttca ctatggcatt tccccgccaa
    12661 ttcaaggagc ttatttcgtc catgcaagat aatgcctgac cataacacaa tcattaagca
    12721 acttattacc caaatgggcc gttcactcat taatgcggca ttcacgatga ttaccagggc
    12781 caaccctgtc aacgtcatca taatcataaa acggtgagaa ccaggatcaa tattaaataa
    12841 taaaccggcc agcatactga ttaaaaacat cgcgcccgca gtgctgaaca cgacgataat
    12901 ggcatataca ggaactaaac ttgcaacgac aatcgccacc aaagagagta aaaacagata
    12961 tattacgcct gcgatggcgg catttatctt gttgatatcc ttaattaagc catacataca
    13021 aagtaataat aaccctatgg cgacatacat tggtggtgaa tcatatttgg ttgccgtaat
    13081 ttcgagatac ttagcccccc aggccagccc tgcggttgcc accaaaccta aagcggtaat
    13141 gagccatata ccgtttaata tcctggtctc gtgctgaaaa tctaatttat tgctgttttg
    13201 caagtatgat tccattcttt tagttgtcct tatagaaaaa tattaatatt catcatgaat
    13261 aaatttaaac ccacgcatac aaaatacttt tgtatttatg gatacggaaa actcttccgc
    13321 tcggccataa ggcgtatttt atacctggta cttgttaagt gtcgttttta gtaagaaaaa
    13381 aaatcccgac acatggccgg gatattgatg gtgaaaaaag aattaacgac gattgccaaa
    13441 aattcgcagc aacatcagga acaggttgat gaagtccaga tacaaggtta atgcgcaaag
    13501 aatggaatat ttgcgcaggt tcgacgcgtc gcgggtatct atctgctcac ccatattttt
    13561 cagtttctgg gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtgac
    13621 tgcccacatc aacgcttcgc ttttcagcca gaagttgacc agcgatgcca acacaatgcc
    13681 gattaacgcc ataaacagca tattgccaaa gccacttaaa tcgcgcttcg tggtgtaacc
    13741 gtacaggctc attgcgccga acatcccggc agtaacgacg aaggtactgg cgatagaagc
    13801 agcggtataa acaatgaaaa tactggaaag cgtaagaccc gtcagcgccg aataaagcat
    13861 aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac
    13921 caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga acaacagctc
    13981 catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca ataagccaac
    14041 ggtcatccag ccatagactt gcgccatata agtctgcaag ccagcccggg gttgtacaat
    14101 tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa
    14161 ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt
    14221 gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aactaccagc gttttgccgc
    14281 ctgctgatcg ctctcccgag cttcgaccca gcggtcgccc tccggcgtgg cttcgcgctt
    14341 ccagaacggt gcgcgggttt tgagataatc cataataaac tgcccggctt caaacgcact
    14401 gctgcgatgc gcactggtga caccgataaa aacgatttca tcgcccggcc ataattcccc
    14461 gatgcggtga atcaccgtga cgcgccccag cggccagcgg ttacgcgctt catcgacaat
    14521 ttctgccagt gctttttcag tcatccccgg atagtgttcg agggttaatg ctttgacgct
    14581 gtcgcccagg ttatggttgc gcaccttacc ggtaaaggtg actaccgcac cgtcttcgtc
    14641 acgctccgcc agccacgggt actcttctcc tacgctgaac ggctgcggac caacaacaat
    14701 tttggtttct gccatcttaa cctccggtta ccggcgggaa gaaagctact tcgtcgccgt
    14761 cagtcagcgg atggtcaaaa ctcaccagcg tctggttgac ggcagccagt aatttgccat
    14821 cttccagcgc cagcgcccag cgatcgctct gcgcagccag gtgctggcgt aacgcttcaa
    14881 cggttgggaa atccgcagcc acttcggttg catctgttcc taccaactcg cgcacctggg
    14941 cgaaaaaaag aactttaatc atccgcttcc accttaaagt cacccgactt gccgccgctc
    15001 ttcgccagca aacgtaccgg accaatcacc atatcttttt gcaccgcttt gcacatgtca
    15061 taaatggtca gcgccgccac ggaggccgcg gttaacgctt ccatttcgac accggttttc
    15121 ccggtcaggc ggcataaggt ttctatacgc acccgattgt gctccggctc ggcctgtaaa
    15181 ttgacttcaa ctttgctgag catcagcgga tgacacagcg ggatcagatc ccaggtgcgt
    15241 tttgccgcct gaataccggc aatacgcgca gtggcaaata cgtcgccttt gtggtggcga
    15301 ccatcaataa tcatcgccag cgtctcgctg cgcatggtga caaaggcttc cgcccgcgct
    15361 tcacgcacgg tttccgcttt ggcggagaca tccaccatgt gcgcttcgcc agcggcgttg
    15421 atatgggtca gttgcgacat acttatttct tcaaatgtgg atggaaatta cacggacgcg
    15481 tacgggcatc cagctgcggc gcgatgatat tttcccatgc ggtacggcac gctttggtcg
    15541 aacccggcat ggcgaaaatc agcgttttgt tagcgacgcc cgctaccgca cgagattgca
    15601 acgtggaagt gccaatctct tcaaacgaca acatacggaa cacttcacca aaaccttcaa
    15661 cttcacggtc gaacaacggc agcaatgctt cgggagcctg atcaccttcc gtcaggccag
    15721 tcccccccgt aatcaacacc acttgtacat cgtcgctggc gatccacgca gatacctgag
    15781 cgcgaatagc gtagcggttt tctttcacaa tggctttatc gacaacgtga tggcccgctt
    15841 cttgcgccga atcgcgcaaa tagtgaccgg aggtatcgtc ttcttcaccg cgacgattag
    15901 aaaccgtaag aatagcaata cgggtcggga taaattcagt gcttacctga ctcatctgat
    15961 ctctcctttt gacgttttag ccgccaatgt acgataagtt ttgcgtaata ccggtgttgt
    16021 tttgatgcag gaaatgggtc tgtttcttct cccgcagcgc cgctgaaata cgcgcttcca
    16081 gcgcctgttg ctgggtatcg tcttccagca gatcgcgcag gttaacgccg ccttcaccaa
    16141 acaggcagag atggagttta ccaatggagg aaacgcgcag acggttgcaa gtggcgcaga
    16201 agtctttttc atacggcatg ataaggccaa tctctccggc gtaatccgga tgacaaaaga
    16261 cttgcgcggg accgtcgctg cgttgacgta attggtggat ccagccgcga cgcagtagct
    16321 cgtcacgcag aacctgacca gagatgtgat gcttacggaa aagctcgctg ccctcgcccg
    16381 tttccatcag ttcgatgaaa cgcagctgga tagggcgatg ctggatccag ttcagaaagg
    16441 tgtcgagctg gtgatgattc acatcacgca tcagcacggt attgactttg accttctcaa
    16501 aaccggcctc aaatgcagca tcgatccctg ccatcacctg attgaattta tcctggccgg
    16561 taatggcatg aaactggcgg gcgtccagac tgtcgacgct gacgttaatg ccagtaagtc
    16621 ccgcatcgcg ccagttcgcc acatcgcgtt ccagacggta accattggtg gtgaccgcta
    16681 tctggcgaat agcgtcgttt tctcgcacag cggcgatgat atcggtaaag tcgcggcgta
    16741 aagacggctc accgcctgtc aggcgcactt tttcggtgcc cagactggcg aaggcgcgcg
    16801 taacccggcg aatttcatcg acggtcagaa agcctttatt ggtgacgccg ctcggtttgt
    16861 agccatccgg caggcagtag gtgcaacgaa agttacacac atcggtaatc gacaggcgca
    16921 agtagtaaaa cttacgcgca aatgcatcag tcagttgtga agccatgtac acctttccag
    16981 atacgggagg cgaagtcatt tcttccttcg ccctggtggc aatctttacg agcattgtca
    17041 cggccaaagc accatatcag ttgacccagg tgcagaggct agagtgttta gtggttaagc
    17101 cgatactagc gtgtaaatga gatttttacc atccacactt tcgctatgta atcatgtata
    17161 tagcgtcctg atcgtgctat ttcgctacag aataagggaa aatggcggtt ttactttgat
    17221 atacatcatc agggaggcat agcgtgcatc gtcctttaga ggtagaagaa aatagtttgt
    17281 atcctcaata ttggcaggtt aattgctgtt tccccgcaat ttgcgctact gtagcgcgga
    17341 taatctgact ccaggaaact atatgcgcaa tcgtacgctg gctgatcttg atcgtgtcgt
    17401 tgctctcggc ggagggcatg gactgggacg cgttctctca tcactttcgt ctttgggttc
    17461 tcgtttaacg ggtatcgtca ccaccacgga taatggtggc tcgacggggc gtattcgccg
    17521 ttcagaaggc ggcattgcct ggggcgatat gcgcaactgc cttaaccagc tgataactga
    17581 acccagcgtc gcttcagcaa tgtttgaata tcgtttcggc ggtaatggcg aactttccgg
    17641 gcacaacctt ggaaacctga tgttgaaggc actggatcac cttagcgtgc gacctcttga
    17701 agcgatcaat ctgattcgaa atctgctgaa agtggacgca catttgattc cgatgtcgga
    17761 gcatcctgtt gatctgatgg cgattgacga tcaggggcat gaagtttacg gcgaggtcaa
    17821 tatcgaccag ttaactacgc cgattcaaga gttattgtta acgcctaatg tacccgcaac
    17881 gcgtgaggcg gttcacgcta tcaatgaagc ggatctcatc attattgggc ctggcagttt
    17941 ttacaccagc ctgatgccaa ttctgctgct gaaggaaatc gcgcaggcat tacgccgcac
    18001 gccagcgccg atggtctata tcggcaatct ggggcgtgag ttgagtttac ctgcggccaa
    18061 tttgaagctg gaaagcaagc tggcaattat ggagcagtat gttggtaaaa aggtcattga
    18121 tgcggtcatc gtcgggccaa aagtggatgt ctcggcggtg aaagagcgga ttgtgattca
    18181 ggaggtgctg gaggccagcg atattccgta tcgtcatgac cgccagttgt tacatagcgc
    18241 actggaaaag gcgttacagg ctttaggtta acggaaatcc gaaggaaaat tccggcttcc
    18301 taatgaagac aaagtgcgcg ttgtttatga cggatgcggc gtgaacgcct tatccggcct
    18361 acaaaccgcg ctaattcaat gtattgcaga atcatgtagg cctgataagc gtagcacatc
    18421 aggcaatttt gcttttgtca tctgtcttct tcgttatcct gtcacgaagc cgcgataaac
    18481 aattcacgca gctgatgcaa ctggtcacga atttgtgccg cttcttcgaa ctccagattt
    18541 tgcgcgtgtt gcatcattaa cccttccagc tcatggattt tctgttgcaa cgctttaggc
    18601 gacatatcca tcggcacatt atccggctca acaatcggtc gcgattttcc tctgcccttc
    18661 gctttggttt tagcaatgtt ctgccccaac gccaggatat cgaccacttt cttgttcaag
    18721 ccttgcggcg taatgccgtg ttcctcgttg tacttctgct gtttctcgcg gcgacgttcg
    18781 gtttcgccaa tcgctttcgc cattgacggg gtgatcttat cgccgtagag aatcgcttta
    18841 ccgttaacgt tacgcgccgc acgaccaata gtctggatca acgaacgttc agaacgcagg
    18901 aagccttctt tgtcggcgtc gaggatcgcc accagcgaaa cctccggcat atccagacct
    18961 tcgcgcagta agttgatccc caccagcacg tcgaactcgc ccagacgcaa gtcgcggata
    19021 atctccatac gttcgacggt gtcgatatct gagtgaagat aacgcacgcg ctcaccgtgt
    19081 tcttcgagat attcggtaag atcttccgcc attcgcttgg tcagtgtggt gaccagtacg
    19141 cgttcgttaa ttgccgctcg ctgacgaatc tccgaaagaa gatcatcaac ctgtgtcgcc
    19201 actggccgca cttcgataat cggatcaagc aatccggttg gacgcaccac ctgatccacc
    19261 acatcgccgc cggatttttc cagctcataa ttacctggcg tcgccgaaac atagatggtt
    19321 tgcggcgcta atgcttcgaa ctcttcaaat ttcagcggac ggttatccag cgctgatggc
    19381 aggcggaagc cgtactccac cagtgtctct ttacgcgccc ggtcaccgcg atacatgccg
    19441 ccaatttgtg gaatggtgac gtgagattca tcgaccacca gcagcccatc ggcaggcagg
    19501 taatcaaaca gcgtcggcgg tggctcaccc ggtccacgac cggagaggaa gcgcgagtag
    19561 ttttcaatcc ccgaacagta gcccagctcg ttcatcatct ccagatcaaa ctgggtacgc
    19621 tgggtcagcc gctgctcttc cagcagtttg ttgttttcca atagcacttt gcgtctggcg
    19681 gccagctctt ctttgatctc ctccatcgcc tgtacgatgc gctcgcgcgg tgtgacgtag
    19741 tgcgttttcg ggtagatggt aaaacgtgga atggtggaaa caatctgccc ggttagcggg
    19801 tcaaataacg acaatcgttc cacttcctcg tcaaacagtt ccacgcgaag tgcaatgtca
    19861 tccgattctg ccgggaagat atcaatcacc tcgccacgaa cacggaaagt accacgctgg
    19921 aatgcttgat cattacgagc gtattgcagc tccgccagtc ggcgcagaat cgcgcgctga
    19981 tcgataatca taccgaccgt gagatggagc atcatcttga gatataaatc aggatcgccc
    20041 agaccataaa tcgcggaaac agacgcgacc acaacaacat cacgccgctc cagcatcgct
    20101 ttggtggcgg acaaacgcat ctgttcgata tgttcgttta ccgaggcatc tttctcaatg
    20161 aaggtgtcgg aactcggtac ataggcttct ggctgatagt agtcgtagta ggagacgaaa
    20221 tattccaccg cgttttccgg gaagaactct ttcatttcgc catacagctg ggccgccagc
    20281 gttttgttgg gcgcaagtac catggtaggg cgctgaaggt cagcaatgac attggcaatg
    20341 gtgaaggttt tcccggaacc agtcacacca agtaacgtct ggtgcgccag gccatcttcc
    20401 agcccctctt cgagacgtcg aatcgcctct ggctgatcgc cagaaggttt aaaagcggaa
    20461 ttcagtttga acggtttact catgagtcgc tacctgaagg agttgggcgg gcaggtatgt
    20521 aattttactc gtcgtactta attatgccaa caaattatac tggataaaaa aacagttcat
    20581 caccataata tttctgttac agcgtaaact ccgctgtcaa tcatgagtaa aatttactct
    20641 gtggcgagat aaaactcgga ccttaccggg ttatccccaa agcaacggct tttttaacat
    20701 ttgtcaagat
//
LOCUS       DAZFBR010000084        20373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFBR010000084 DAZFBR010000000
VERSION     DAZFBR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.084"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            134..1831
                     /gene="ypdA"
                     /locus_tag="ACHJZ2_003381"
     CDS             134..1831
                     /gene="ypdA"
                     /locus_tag="ACHJZ2_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO4569434.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1846..2580
                     /gene="ypdB"
                     /locus_tag="ACHJZ2_003382"
     CDS             1846..2580
                     /gene="ypdB"
                     /locus_tag="ACHJZ2_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO4569435.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2593..3450
                     /locus_tag="ACHJZ2_003383"
     CDS             2593..3450
                     /locus_tag="ACHJZ2_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4569436.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3453..5948)
                     /gene="ptsP"
                     /locus_tag="ACHJZ2_003384"
     CDS             complement(3453..5948)
                     /gene="ptsP"
                     /locus_tag="ACHJZ2_003384"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4569437.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIIRNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSEPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHGLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5973..7010)
                     /gene="ypdE"
                     /locus_tag="ACHJZ2_003385"
     CDS             complement(5973..7010)
                     /gene="ypdE"
                     /locus_tag="ACHJZ2_003385"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4569438.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7010..8095)
                     /gene="ypdF"
                     /locus_tag="ACHJZ2_003386"
     CDS             complement(7010..8095)
                     /gene="ypdF"
                     /locus_tag="ACHJZ2_003386"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4569439.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(8110..9357)
                     /locus_tag="ACHJZ2_003387"
     CDS             complement(8110..9357)
                     /locus_tag="ACHJZ2_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO4569440.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9379..9705)
                     /locus_tag="ACHJZ2_003388"
     CDS             complement(9379..9705)
                     /locus_tag="ACHJZ2_003388"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO4569441.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9924..10889)
                     /gene="glk"
                     /locus_tag="ACHJZ2_003389"
     CDS             complement(9924..10889)
                     /gene="glk"
                     /locus_tag="ACHJZ2_003389"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO4569442.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11093..12349
                     /locus_tag="ACHJZ2_003390"
     CDS             11093..12349
                     /locus_tag="ACHJZ2_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO4569443.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12464..12790
                     /gene="ypeC"
                     /locus_tag="ACHJZ2_003391"
     CDS             12464..12790
                     /gene="ypeC"
                     /locus_tag="ACHJZ2_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO4569444.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12930..14168)
                     /locus_tag="ACHJZ2_003392"
     CDS             complement(12930..14168)
                     /locus_tag="ACHJZ2_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO4569445.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14504..15706
                     /gene="nupC"
                     /locus_tag="ACHJZ2_003393"
     CDS             14504..15706
                     /gene="nupC"
                     /locus_tag="ACHJZ2_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO4569446.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15756..17945)
                     /locus_tag="ACHJZ2_003394"
     CDS             complement(15756..17945)
                     /locus_tag="ACHJZ2_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO4569447.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18153..18228)
                     /locus_tag="ACHJZ2_003395"
     tRNA            complement(18153..18228)
                     /locus_tag="ACHJZ2_003395"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18193..18195),aa:Ala,seq:ggc)
     gene            complement(18268..18343)
                     /locus_tag="ACHJZ2_003396"
     tRNA            complement(18268..18343)
                     /locus_tag="ACHJZ2_003396"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18308..18310),aa:Ala,seq:ggc)
     gene            18564..18923
                     /locus_tag="ACHJZ2_003397"
     CDS             18564..18923
                     /locus_tag="ACHJZ2_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO4569448.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18925..19317
                     /locus_tag="ACHJZ2_003398"
     CDS             18925..19317
                     /locus_tag="ACHJZ2_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO4569449.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19357..>20373)
                     /locus_tag="ACHJZ2_003399"
     CDS             complement(19357..>20373)
                     /locus_tag="ACHJZ2_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4569450.1"
                     /translation="ATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPRFKKRGQ
                     NDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWYISIQTE
                     SEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKF
                     SNNWQKQKRKIQRLHSCTANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTV
                     SQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHT
                     AKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMI
                     QATA"
BASE COUNT         4979 a         5447 c         5094 g         4853 t
ORIGIN      
        1 gagatgcatt gctaatacac catttatcta tcaatcccct tttaaaattg tggtttttcc
       61 tcaaaaacgg cctgctggtc atcagccttc aggttaccta tcatagaggt ttaatcctta
      121 ttcagagtca cccgtgcacg aaatattcaa catgctgctg gcggtcttcg atcgggcagc
      181 gttaatgctt atctgcctgt tctttctcat ccgtatccgc ctgtttcgcg aactgttgca
      241 caagtcggca cactcaccga aagagttgct cgccgttacc gccattttct cgctgttcgc
      301 cctcttcagc acctggtccg gcgttcccgt agaaggctcg ctggtgaacg tacgtattat
      361 cgcggtgatg tccggcggga ttctgtttgg cccgtgggtg ggcatcatta ccggcgtgat
      421 tgcgggtatt catcgttatt taattgatat cggtggcgtg acggcgatcc cctgctttat
      481 caccagcatt ctggctggtt gtatatcggg ctggatcaac ctgaaaatcc ccaaagcaca
      541 gcgctggcgc gtcggtattc tcggcggcat gttgtgtgaa acgttgacca tgattctggt
      601 gattgtctgg gcaccaacta ccgcgttggg gatcgatatc gtctctaaaa tcgggattcc
      661 aatgatcctc ggcagcgtct gtatcggctt tattgtgctt ctagtgcaaa gcgttgaggg
      721 cgaaaaagag gccagcgccg cgcggcaggc caagttggcg ctggatatcg ccaacaaaac
      781 gctacctctg tttcgccatg tcaatagcga gtcattacgc aaagtttgtg aaattattcg
      841 cgatgacatt cacgccgatg cggtggcgat taccaatacc gatcatgtgc tggcctatgt
      901 tggtgtgggt gaacataact atcagaatgg cgatgacttc attagcccga ctacccgtca
      961 ggcgatgaat tacggaaaaa tcatcattaa aaacaatgat gaagcccacc gcacaccaga
     1021 gattcattcc atgctggtga tcccattgtg ggagaaaggc gttgtgaccg gaacgctgaa
     1081 aatttactac tgccacgcgc atcagataac ctcgtcatta caggaaatgg cggttggtct
     1141 gtcgcaaatc atctccacgc aactggaggt ttcacgcgcc gagcagctac gtgaaatggc
     1201 aaataaggca gagcttcgcg ccctgcaaag caaaattaat ccccatttcc tgtttaacgc
     1261 tttgaacgct atttcatcgt cgatccgtct gaatccggat accgctcgcc agctaatctt
     1321 taatctgtcg cgttatctgc gctataacat tgaattaaaa gacgatgagc aaatcgatat
     1381 caaaaaagag ctgtatcaaa ttaaagacta tattgccatt gagcaggccc gctttggcga
     1441 caagctgacg gttatctatg atattgatga agaggtgaat tgctgcattc ccagcctgct
     1501 gatccagccg ttggtggaga atgccattgt ccacggtatc cagccttgca aaggtaaagg
     1561 cgttgtcacc atcagcgttg cagagtgcgg caatcgggta cgcattgcgg tgcgggatac
     1621 cgggcacggc atcgatccaa aggtgattga gcgggtcgaa gcgaatgaaa tgccgggcaa
     1681 taaaattggc ctgctgaatg tccatcatcg cgtgaagtta ttgtatggcg aggggctgca
     1741 tatccgccgc ctggaaccag gaacggaaat tgcgttttac attcctaacc aacgcacccc
     1801 agtcgcctca caggctacgt tattgctttg agccggagtg atattgtgaa agtcatcatt
     1861 gttgaagacg aattcctggc acaacaggaa ctgagctggc taattaaaga gcacagccag
     1921 atggagattg tcggcacctt tgacgacggt ctggacgtgt tgaagttttt gcagcataac
     1981 cgcgtcgacg ccatttttct ggatatcaat attccgtcgc tggatggtgt gttgctggca
     2041 caaaacatca gccagttcgc ccataaaccg tttattgtgt tcatcaccgc gtggaaagaa
     2101 catgcggtag aagcgtttga actggaggcg tttgactaca ttctaaaacc gtatcaggag
     2161 tcacgcatta ccgggatgct gcaaaaactg gaagcagcct ggcaacaaca gcagaccagc
     2221 agcacgcctg ccgcgacggt aacgcgtgag aatgacacca ttaatctggt gaaagatgag
     2281 cgaataatcg tcacgccaat taacgatatc tattacgccg aagcgcacga gaaaatgacc
     2341 tttgtttata cgcggcgtga atcctacgta atgccgatga acattaccga attttgcagc
     2401 aaactgccgc cgtcgcattt tttccgctgc catcgctcat tttgtgtcaa tctgaacaaa
     2461 atccgcgaaa tcgaaccgtg gtttaataac acctatattc tgcggctgaa agatctggat
     2521 tttgaagtgc cggtcagccg cagcaaagtt aaagaattcc gccagttaat gcatctttaa
     2581 tcgggaactt tcatgaaagc accaggcttg cccgccgatc aacaattttt cgccgatctg
     2641 ttcagcggcc tggtgcttaa cccgcaacta ctggggcgcg tctggtttgc cagccagcct
     2701 gcctcgttgc cggtgggcag tttatgtatt gattttcccc gtctggatat cgtgctgcgc
     2761 ggcgaatacg gcaatctgct ggaagcaaag cagcaacgtt tggtggaagg agaaatgctg
     2821 tttattccgg cgcgcgcggc taatttaccg gtcaacaaca aaccggtgat gctgttaagc
     2881 ctggtgttcg ctccgacctg gcttgggtta tcgttttacg atagccgcac cacgtcattg
     2941 ttgcatcctg ctcgccagat ccagcttccc agcctgcaac gcggtgaagg tgaagcgatg
     3001 cttaccgccc tcacccatct tagccgttcg ccgctggagc aaaatatcat tcagccgctg
     3061 gtgttaagtt tgctgcatct ttgccgtaac gtggtgaata tgccgccggg caattcgcag
     3121 ccgcgcggcg attttctcta tcacagcatt tgtaactggg ttcaggataa ttatgcccag
     3181 ccgctcaccc gcgagagcgt ggcgcagttt tttaatatca cgcccaatca tctgtcaaaa
     3241 ctgtttgctc agcatggaac gatgcgtttt atcgagtatg tgcgttgggt gcgaatggcg
     3301 aaggcgagga tgattttgca gaaatatcat ttgtcaattc atgaagtggc acagcgttgc
     3361 ggttttccgg atagcgacta tttttgtcgc gttttccggc gtcagtttgg tctgacgccg
     3421 ggagagtaca gcgcccgttt tcagggctaa cgtcagaagg ttaattctgt ttccagcagc
     3481 gtcaggatac tttgtgcatc ttgcgcggca aacaacgact ggcggaagtt tttattcacc
     3541 agtttacgcg ccagctgcga gaagactttc acatgattca tcccttcgtt agcacccagc
     3601 gtcagcatga tcaccagttc gacttcgccc atttctgact gccagtcaac cggtttcgcc
     3661 agccgggcaa tgctgatact ggaatgacgg atccactgag atttggtgtg cgggatcgcc
     3721 acgccaaaac caacgccagt ggtgacaatc tcttcccgct gccagacatc ctcttccagc
     3781 tcgaacggat gttcagtgcg cccgttaacg ccgaggttac cgcacaggaa ctggatcgcc
     3841 tgctctttat tgctaaaggc ctgatcaacg aagatattct ccagcgccag cagcgggcga
     3901 acgtcttctt ccggcgtaaa ggcggtgagt aacgcttcaa tttcctgcgc gctgcgacat
     3961 tcacacgcct gacgtgccag ttcccgacac gcctcgctat ccagttgacg aagctggctt
     4021 ttcaccgccg gaatgcgcgg gctactcata ctcagctcat ccaggcccag cccaagcagt
     4081 agcggcagat aacggctttc accgcccagt tcaccgcaaa tgcctaccca tttgccccgc
     4141 tgatgcgcag tggtaactat ttgctgcaac atgcgcagga acgatggcgt aatcgggtta
     4201 tatagcggcg atacgcgcgg gttattacga tcgaccgcat acagatactg ggtcatatcg
     4261 ttggagccga tactgaagaa atcgacctca tcgcagaagt gatcgatgat gtagcacacc
     4321 gacggcactt ccaccatgat acccagcgta atcgtctctg catgacgcag gccatcgcgc
     4381 ttaagctcaa cgatcgcttt ttggatctcg cccttcaccc ataagatctg atcgaggccg
     4441 tggaccatcg ggatcatcaa ctgggcgttg ccgaaactgg cggcgcgcaa aatggcccgc
     4501 agttgagtgc ggaacaggcc agcaaattcc gggtaaatac gtaccgcgcg atagccgagg
     4561 aacgggttct cttcctgggg aatattcaga taaggaatgc ttttatcgcc tccgatgtcc
     4621 attgtgcgga agataatcgg cttgtcgccc gctgccagta gcacctgctg gtaggcttca
     4681 aattgctcct gctcgtccgg ctcgctgtcg cgatccatat agagcatttc ggtacggaac
     4741 agcccgacgc cttccgcgcc gttggcaaac gcgccaggcg cttccagagc ggtgccaata
     4801 ttcgccgcga tatcaatacg cttgttatca cgggaataag ccagctgcgc ggcagcctgc
     4861 gcctgttgtt tttggcgttt atccgccagc gtctgcgcga cctgataata accgctcacc
     4921 gcgtcattcg ggttaatcgc cagcacgccg cactgggcgt caagcactgc aggttgcccg
     4981 gcataacgcg cgatcgcatc cagcggcagg ccgctcagta ccgggatcgc cgaggcgcgg
     5041 gccagaatca gggtatgcga ggtgcgcccg gttttctcca gaatcatgcc cgcaagattt
     5101 tttaaatcca ggcttaaaaa ctggcttggg gttaaatcct cggccaccag aatagtcggt
     5161 ttttccagca ccagattgtt gcgcggcttc agttccggcc aggtaatatg cagcaactgt
     5221 tcgctaatat cgcgaatgtc gctaacacgt tcgcgcagat aatcgctggc agaagcagaa
     5281 agtttggcgc aaacctgctc catatttctg atgatcgccg cccccagccc ctgatgctgt
     5341 tctgtcatca ggcgacggat attgcctgca aattcatcat cctgaatcag cgacaaatgg
     5401 gcgctgagga tagttttgct ttcgccgtca cgctcacgca attgctggtt cagttgctcg
     5461 gcaagcgttg ccaggctgtg ctccagccgg gtggaatctt gcgcactggc ggggattgcc
     5521 cgataactgt cgaggctgtc gctttgtaac agggtcagcg tacccacgcc gacgccgctt
     5581 gccagcacat tgccgtacag taaatccggg ttaaggcggc ttaatgaacg cggcagcgga
     5641 tgcgccgtca gttctgcctg cgtaggctga acgctgtcgc tgtcgataaa gcgcacctgg
     5701 atgtactctt ccagcacgcg ccgcgcctgc tcttcatcgc tgccgctaat gttcaggctg
     5761 caactgtcat taaatagggt gccggtgcca atcagcgcca gcgagctttt ggcatcggcc
     5821 tttgcgttct ggcgatggtt aataaaagtg atttcgcttt gccactggct gcactgttct
     5881 ttaagttccc acgccggacg agcatgtaga ccgttaggca aaggacagag aaattgaatc
     5941 gttaacataa ccgctccggg ttaatcagga gatcatctga aatccgtcag ttgaacaacc
     6001 gtctcacgcg taagacgttg aataagggca gataaaagtt gctccatctg caaaatgtca
     6061 cggcaatcgg caatcgacgc ggcgcaatgt ccgtggcggg tggcaggccc catcaccact
     6121 gtgggtacgc cagtaccggt taagtgcacc gctccaccgt ccgtgccgcc gttactgaac
     6181 atatccgcct gtaacggcac gccaatttct gccgccacgg tttcgatcca ggcggtgagt
     6241 tttggcggcg caatcagtga cttgtcgctt aacaccagca tcgggccgtt accaatctgg
     6301 cgatggttag ccgcgccata attaaagttt ttcgcccagc aggcggtatc aaggacaatg
     6361 gcgacgtccg gcgacaccgc gcgggtggca gtttgcccgc cgcgtaaccc cacctcttcg
     6421 ctggaactgg ccaccagcca cacttccgca ggcagttcag cgctgtgtag ttcgcgcagt
     6481 aacgtcacca gcaggtagca accgaggcgg tcatcaaagg ctttccccat cacccgctgg
     6541 tgggggagaa cctgaaaagt ggtatcgaac gtgacgcgat cgcctggacg aattcccgcc
     6601 tgcatcactt cgtcatacga gcgcgcgcca atatccacgc gcatggcgct gacatcattc
     6661 ccctgccggt cgccgtcaag caggcctgga attttgcact cttcacgggt ggtgatgcgc
     6721 accggctgca gctggcgggc agccatgcgt acattgccaa ccggcagcac atcaatcgcc
     6781 ccttcgcggg agatgctgcg caccataaat cccacttcgt ccatatgcgc acagatcatc
     6841 accttcggac ctgtcgattc attgaggcgg atcagcaccg atcccagccc atcaaatcgc
     6901 acttcttttt gcaggcgatc cgcttcttcc agcaggatct gccgcacttc ctgttccgag
     6961 gaggcgatcg catctgcctc gctcaacgct ttcaatagcg ataaatccat tatgcctctc
     7021 ccgtgagcaa cactgttttc ggcatggcgt agagcacttc tgcgccttgc ggggtgacca
     7081 gcacaacatc ctcgatgcgc acgccccctt gccctggcaa ataaatcccc ggctccacgg
     7141 tcagtaacat gcctggctgt agcgtcgtgg tgtcccgcgg tgaaaaacgc ggatcttcat
     7201 gaacttcaat gccgatagca tgagcggtgt tatgaccgaa atagtggcta aaacctgcct
     7261 cggtaatcac ccgacgcgcg gcttcgtcaa cctgctggca gcgcacgccg gggcgaattg
     7321 cagagattgc tgcgagctgt gcctgcaaaa caatctgata gacgttaaac agcgggtgag
     7381 attcggcgct caccccttcg ccattcacca gcaaggtgcg cgtcatatca gagcagtagc
     7441 cctgatagag cgcaccgaaa tcgagagtga caaactcgcc cgctgcaaca atcttgtcgc
     7501 tggctttgcc gtgcggcagc gccccacgcc agccactggc gacaatggtg tcaaaagagg
     7561 ttttttctgc gccctgctgg cgcataaacc actccagttc agcggctatc tcgcgctcgc
     7621 tcatccccgc ctgaataaag cggcgaatat gctctgcacc gcgatcggca atcccacagg
     7681 caaggcggat tttctccacc tcctctggcg ttttgatttg ccgcagcaca tccggcgtgg
     7741 cgctaaccag tttcgcattg agttcagact gccagcgatg cgcggtttcc caactcacct
     7801 gctggccttc aaaaccgagc gtttgcaact gctcatcggc aatgatttgc ctggcgatgg
     7861 ttgtaagcgt gtgcgtcgcg tcaagcaaat gcagctggta accttgcgtg cgggcttcta
     7921 catccgcgta atagcgcgag tccaccagaa tgtgcgcact ttcacggcta atcagcacat
     7981 agcctgatcc ggtggagatc cccagatgcg gctgtttgtt ctgccgtgag gagagaagca
     8041 ccgcatccag ttgttgcgcc ttaagccagt cgcgcagcga agcgagtaat gtcatctgga
     8101 ttttccttct tacaggctat cgattaacaa tttgccttta cggaacatca tcagacgcag
     8161 gaacaccacc atcagcgcag ttatgactgc ccccagcgcg atccccgcca tatagacgcc
     8221 caggttagtg accagcggcc atgcccagat agcggattcc gggaaccatt gcactgcgcc
     8281 cagccagaca gcggcggttg agccgacaat cgcgccgacc atatacgacg gaatggcggt
     8341 gatggggctt tccagcgcaa acggaatcgc accctcactg atccccataa aggcaaggaa
     8401 catcgcggtc ttaccctgcg gataaagttg tgcgttgaac aggcgtttgc cggttaaacg
     8461 acggtcaata atggtcgcca gacccagacc aatcggcgga ataacgatag cgatagaacg
     8521 agcggtgacc ggtaatacgt ggtcagtggt aaagctgaag gcaacaaaac ctgcggcttt
     8581 gttgatcggg ccaccaaggt cgattgctgt cgccgcggcg atccccattg cgtacatcag
     8641 cgcgcccttc tcacctgcgg cagtcagcac agtacggata ccgccgttga tccagccccc
     8701 aaagggggtg atgacgtagt acatcgccag catgacaaaa atagcggaaa gaatcggtag
     8761 caaaaatgtg gttttgaacg ctaacaggaa atccggtaac tggattttct ggttcatcca
     8821 tttcacaagg taaccggcaa cgatagaaat aatcagcgca ccaatgaagg tggacggcac
     8881 cggcgaagag gtcgcccatt gcatagtgct gggatcgaag ttcagcagtt gcgtcggctg
     8941 ggtagacatc aagccgccga taaaaccagc cggaaacgcc agtttgccac cgatagagtt
     9001 cgccacaaaa gcagcaaaca tcggaatggc aaaaccaaac aacacgccgc cgaaggactg
     9061 cgacaaccag gcaaatttca gtaaggagag gtcgaaaccg gagaatttcc cgctattaag
     9121 agcatccatg atgccgatct cagcaggaat tttaagccag ctataagcaa tcaactggct
     9181 gaaagcgagg atcacaccgc ccataattaa ggtcggcacc atgcgtgaaa tccctgacat
     9241 cacatgctgc gggagttcac cccagaagct gcttttagag gcctgcggat tcttaactgc
     9301 cgctgccgca ccggatgcgc caggcacaac ggttgcactg cgttttttaa tggccataac
     9361 aattatccct gtcgaatatt actgttgttc agaggcaatc atctcttcga tttctttgat
     9421 gatgcccgca gcgtttttaa ttgcgtcctg caaagtgatt tcataaacgt cgcgtgattc
     9481 gaaacgttcg ttatcttccg gggtaactgc cacggagtgg atgatgatgg tcgcttcggc
     9541 gatatcctgc gccgtcaggc gattctggat accgtccgcg ccctgggttt caattttcac
     9601 ttcataaccg gcttctaccg ccgcttcttc cagcgcctga gcggccataa aagtgtgagc
     9661 caggcccatt gggcaggcac ataaggcaat cagtttctta ctcatcgtta attcctcgtt
     9721 cagtgatagt tgacgagtga aattatgaac gtgcaaaaca aatcgccgtt accagacaaa
     9781 taatgcattt actggagaat taatcccgct gtacggaagt gtgacgcagg tcggccttgt
     9841 gaggggacac aaagaaaagt tgaattaact gtatgattta aaagattatc gggagagtta
     9901 cctcccgata taacaggaag gatttacaga atgtgaccta aggtctggcg taaatgtgca
     9961 ccggaaccga gaagacccgg attgtcatgg acgatgagat acaccggaat atcatggaca
    10021 tattctttaa agcgcccttt atcttcaaat gcggcacgga aaccagaggc tttgaagaac
    10081 tcaaggaagc gcggcacgat accgcccgca ataaacacgc cgccaaatgt cccgagattg
    10141 agcgccagat tgccgccaaa acggcccata atgacgcaaa acagcgacaa cgcacggcgg
    10201 caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat cttttggctt gagattttct
    10261 ggcaggcggt tgtcggcttt aacaattgcg cgatacaaat tcaccagtcc tgggccagaa
    10321 agcacgcgtt ccgccgaaac atgaccaatt tccgcacgca atatttcgag gataatggcc
    10381 tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt cgcccggcaa gcttacccaa
    10441 cgcttatcga catggaccag atgcgcaacc ccaagccccg ttccggcacc gtaaaccgca
    10501 ataggcttac cttctaccgg ttctgcgcca ccaaactgaa tcagatgctc ttttttcagc
    10561 atcgggatcg ccatcgatac agcggtaaaa tcgttaataa tttccagatg gctaaaaccg
    10621 agattctttt tcatttcggc aattgagaac gcccaggtat ggttggtcat tgccacccag
    10681 tctccggtaa tcgggcaagc gatggcaata cagccgtctt tcacctcgac cttatgttct
    10741 tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat caagccctga ataggtctta
    10801 gcctgcgaga tttcaccact ggcaatatca cacagagcaa gacgtgcgtt ggtgccgccc
    10861 acatcaccga ctaatgcata ctttgtcatt cttcaactgc tccgctaaag tcaaaataat
    10921 tctttctcac actgtaaata cctgggggca taacaacaac gccggaaagg caggctccct
    10981 gtaaatatcg atctgggtca cacaattact ttatcgtttc agcaccaatt gcagcgatgc
    11041 ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat aaggaataga acatgctcca
    11101 tccgcgagcc agaaccatgt tgttattatc gctccccgcc gtggcaattg ggattgcgtc
    11161 cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta ttacagaatt tgctctggca
    11221 acgactgccg ggaactctgg ggatagccca ggattcaccc ctctggatca tcggtgtatt
    11281 aacgctaacg ggtattgcgg tggggttggt tatccgtttc agccagggtc atgccggacc
    11341 agaccccgcc tgtgaaccgc tgatcggcgc accggttccg ccctctgcgc taccaggact
    11401 tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg aacatccgat
    11461 catgaccgtc aatatcgccc ttgcggttgc cattggcgct cgtctgttac cgcgcgtcaa
    11521 ccgaatggag tggactattt tagcctctgc cggaaccatc ggcgcgctgt ttggtacacc
    11581 tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc agtagtgaag ttccgctatg
    11641 ggatcgtctt tttgcgccgt taatggcggc agcagctggt gcacttacta ccggattatt
    11701 tttccatccc catttttcac tgcccattgc gcattacgga cagatggaaa tgaccgatat
    11761 tctcagcggt gcaattgtcg cggcgattgc catcgcagca gggatggtcg ccgtatggtg
    11821 cttaccacgg ttgcacgcga tgatgcatca aatgaaaaat ccggtgctcg tgctgggtat
    11881 tggcggattt attctcggta ttctgggggt tattggtgga ccagtttcgc tgtttaaagg
    11941 gctggatgag atgcagcaga tggtggcaaa tcaggctttc agcaccagcg attacttttt
    12001 gctggcggta attaaacttg ccgccctggt cgttgctgcc gccagtggct ttcgcggtgg
    12061 gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg ttgatgctgc atgagcacgt
    12121 tcccgccgta ccagcggcaa taaccgtttc ctgcgctatt ctcggcatcg tgctggtggt
    12181 aacacgcgat ggctggttaa gtctttttat ggcggcagtc gttgtaccca ataccacatt
    12241 gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg ttattagcag gtaagccgat
    12301 gatgatggtc aatcgtccga agcaacagcc accccacgat aacgtttagc aaaaaatgct
    12361 ctcctttatg atttaagagt tatggcgatg atacggggct ttacctcccc gtaatattgc
    12421 gttaacaggc cgctgacggc ttcgatcgtg aaggagaata acaatgttca ggtcactgtt
    12481 tctggcggcc gccctgatgg catttacccc gcttgcagca aacgcaggcg aaatcaccct
    12541 actgccatca atcaaattac aaattggcga tcgcgatcat tacggtaatt actgggacgg
    12601 tggtcactgg cgcgaccgtg actactggca tcgcaattat gaatggcgta aaaaccgctg
    12661 gtggcgtcat gataatggct accatcgtgg ctgggataag cgtaaagcat atgagcgtgg
    12721 ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga aaaggccgcg gacatggaca
    12781 ccgccattaa acacattcgc caatggagca caaggcctga tgcgacgcta gtgcgtctta
    12841 tcaggcctac aaaccggctc tttaacgcgt agggcggata aggcgtttac gccgcatcgc
    12901 caatagtgcc agatgcgacg ctcattcaac tacaatccca gcgccgtccc caccaataac
    12961 cagatattca gcgccacgac cagcactaca atcacccagc ctgtctgttt tacgcgtttg
    13021 ctgttcacca gatcgcccat caacttgctg tcactggtga aaatcagcag tggaaccagc
    13081 gccagggcga taccaaaact taacagcacc tgactcataa ccagaatccg tgtcggatct
    13141 aatcccatca gaatgacaat aaatgacggc agcatggtga ctgtacgacg cacccacagc
    13201 gggatatgga agcgaatgaa cccctgcatc accacctgcc ctgccagtgt ccccaccacc
    13261 gttgaggaca gtccggcagc aaccagactt aacccaaaga ccgttgccgc agcatggctt
    13321 aacagcggtt gcagcgtcaa ataagcctca tcaagatcgg caacaccagt atgaccagaa
    13381 aagtggaacg ccgccgcagc tgtagccatc atcgccagat tgacaaaacc ggcaatagtc
    13441 atggcgatag ccacatccca tttggtggcg gaataacgtt gttgacgcga accgccatgt
    13501 aaatgctgag tgagcgaaga gtgcaaataa atcacatgcg gcataatcgt cgcccctaac
    13561 acgcctgctg ccaggaagac cgcctccgaa gtaggtaaac tcgggatcac cattccttta
    13621 cccagctgcg ccaggttagg ctgggagaaa atcaactcga caatgtaagc cgcggcaaca
    13681 aacaacagta acccgccaat cactttctcc agcggttttt gcccgcgacg ttgcagcatt
    13741 aaaatcagga aagtcgcgat ccccgtcagc accgcgccct gcaacaacga aacaccaaga
    13801 atgagtttaa aaccgatcgc cgcaccaata aattccgcca ggtcggttgc catcgcaata
    13861 atttctgcct gaacccaata gaaccacact acgggacgcg gatagtgatc gcgaatctgc
    13921 tccgccagat ttttaccggt ggcaatccct agtttggcag agaggatctg aatcagcatc
    13981 gccatcaggt tggcccaaac gacaacccac agtagctgat agccgaagct ggcacccgcc
    14041 tgaatattgg tcgcaaagtt accgggatcg atataaccaa tcgccgcaat gaacgcaggt
    14101 cccattaatg cgagcctcat cttgcgcgcc gcccgtccgc tgctactctc aacgcgatag
    14161 ttcgtcatct tgtgcctcta aaacatagcc tttgctatgt ttcatgctat gccaaacgag
    14221 aatgattatc aaattcattt aaatgggttg tggtgatttc tctgatagac caggattatg
    14281 actacgaaaa gattgatgac ttgaatgtta tggttatgtt taatgttagc acatttacat
    14341 aactttcagc ttccatacac aacatagcag aaatgtatga cagctcacta tttttgaagc
    14401 ttgtcacagg acgtcattat agtgtgtgtc agatctcgtt ttccttaacc atgttacata
    14461 gaatgtgcac ggaaatttaa cctgcctcat atttggagca aatatggacc gcgtccttca
    14521 ttttgtactg gcacttgccg ttgttgcgat cctcgcactg ctggtaagca gcgaccgcaa
    14581 aaaaattcgt atccgttatg ttattcaact gcttgttatc gaagtgttac tggcgtggtt
    14641 cttcctgaac tccgacgttg gtttaggctt cgtgaaaggc ttctccgaaa tgttcgaaaa
    14701 actgctcgga tttgccaacg aagggactaa cttcgtcttt ggtagcatga atgatcaagg
    14761 cctggcattc ttcttcctga aagtgctgtg cccaatcgtc tttatctctg cactgatcgg
    14821 tattctccag cacattcgcg tgttgccggt gattatccgc gcaattggtt tcctgctctc
    14881 caaagtcaac ggcatgggca aactggaatc ctttaacgcc gtcagctccc tgattctggg
    14941 tcagtctgaa aactttattg cctataaaga tatccttggc aaaatctccc gtaaccgtat
    15001 gtacaccatg gcagcaacgg cgatgtccac cgtttctatg tccatcgttg gtgcatacat
    15061 gaccatgctg gaaccaaaat acgtcgttgc agcgctggta ctgaacatgt tcagcacctt
    15121 tatcgtgctg tcgctgatca acccttaccg tgttgatgcc tgtgaagaaa acattcagat
    15181 gtccaacctg cacgaaggtc agagcttctt cgaaatgctg ggtgaataca ttctggcagg
    15241 tttcaaagtt gccattatcg ttgccgcaat gctgattggc tttatcgccc tgatcgccgc
    15301 gctgaacgca ctgtttgcta ccgtgactgg ctggattggc tacagcatct ccttccaggg
    15361 catcctgggc tacatcttct atccgattgc atgggtgatg ggtgttcctt ccagtgaagc
    15421 actgcaagtg ggcagtatca tggcgaccaa actggtttcc aacgagttcg ttgcgatgat
    15481 ggatcttcag aaaattgcct ctacactctc tccgcgtgct gaaggcatca tctctgtgtt
    15541 cctggtttcc ttcgctaact tctcttcaat cgggattatc gcaggtgcag ttaaaggcct
    15601 gaatgaagag caaggtaacg tggtttctcg cttcggtctg aagctggttt acggctctac
    15661 cctggtgagt gtgctgtctg cgtcaatcgc agcactggtg ctgtaagacc atacataaaa
    15721 aagccgggga taattccccg gcttttttac gcctgttaat cagctaatgg ctgcgggcga
    15781 ccaatcaaat acccttgcag atattgcacc ccgagcttat gcaatagcgc ctgttgctgt
    15841 tgcgtctcga caaactccgc gaccacactc aatgacttcg ctttcgccag atcggtaatt
    15901 gatctcacaa tcatcgcatc cagcgtattc gtgacaatat ctttcacaaa gacgccatca
    15961 attttgatga tatcagcctg caaacgcttt aaccgttcgt agttggcata gccggtgcca
    16021 aagtcatcaa tcgcaatccg gaaaccaaac ttatgcagct gctcgatgtt gtacatgctg
    16081 ctttctgcgt tagaaaacgc ctgctcctcg gtgatctcaa gaatgaccgc ctgcggggag
    16141 atgtgataac gcttaaatag acgaataatc cgcccggcaa tattcttttg cagcagcgtg
    16201 agcggcatta aattaactga aaagcgcggg ccttttttgt cgcaagggtg tgttgccagc
    16261 cacttcaaca aggattccag cacttgcaaa tcaaaacgcg cgctaaggtt aaactgagca
    16321 ataaggggca gaaacttatc cggggtcata atgccgccgt catatttcag tcgcgcgagg
    16381 acctcatcat aaccttcacc ctctttgttg cgaattggct gggcgtagag cagcaaatca
    16441 ccctgatcta acgccgtgcg aatggtattc agcaatagca cctgtttagt ggtctgcccg
    16501 gaaaccatct cctctcggct atccagcgcc agcacatgat gatgtgcgca ggattgctcc
    16561 gccagccagc ttaactgccc caacaagggt tgcagggttt cctgatttcc atcaaaacgc
    16621 ccccaggcag caccatagcc catatccagc ccggtattgt tccagtgaat ttgccgacta
    16681 ttcaggatgt taaccatatg ctggagtcgc ccttccgttt caggcccgct cagcactaac
    16741 agcagttcac tacccggcaa ttgatacaac ttttcgtttt cctgcatcaa tggcagcagc
    16801 gtacggtaaa ttgatcggat acagtgaaca cgcatcatca acccgtaatg acgactcata
    16861 aactcaagat tatcaatgcg caggcagcaa aaactcttgc ccgcctcttg ctccggcgct
    16921 tgttccaacg cacgaaagtt gggcaggagc gttaacggat cggtcagcgc ctgcaaatgc
    16981 cagcggcgat taagccattc actgcgatga taaatgcgca ccatatagag caggcaaacg
    17041 ctaaaggaaa tcagcaccgc gagaataaat gccagcgaat attcggtttc caccccttgc
    17101 aaaaagttct ggttgtaatt cagcaggcaa agcgttgaaa cagcccaagt gagatttaaa
    17161 aacggatagc gaagcttacc gaccccgagg gtaaagatga tgaagaaaac gggtaccagg
    17221 taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca gaagtagcag cacgcttaac
    17281 gctgccagcc agcttaaggt aaatgcgcgt ttctctttgc ccaacgacgg agcgatatcc
    17341 ctgcgccaca atatctgcgc aaagtgggga cttacaatca tgcgagtgag atagtagaaa
    17401 agcatgttgt aaatcagcac agcggtgaaa aggcttagca aatcaacgac cgtgaaaatg
    17461 gcatccgcat cgccgaaaaa ggtggatatc tttagcggga aatcaaagaa gctccccaca
    17521 agatacatgc tgcatttgat gccgatcggc gtcaccaaac caagccagaa cagacgttgc
    17581 cagacatatc ggctggtcaa tccataacgc caacgcgtcc ccagctgcca gcgtaatatt
    17641 gcgcaggcac acaacacgac aaacgtctgg ctaccgagca tgaccgcagt ctgtaaaaaa
    17701 gagagatgaa agttccacag attggtgcaa aacatcccgc ataaaacggg aacgacaccg
    17761 cgccagccaa agataaacaa gattgacagc attacgcaca gtggcatcca cgccagaaag
    17821 atataactgg aatggataat ggcaagtggc gaaataaaac gggatagctg aataagtacc
    17881 acggtgagcg taaacgctag tgtgaatatc ttgatatttt ttatcaggtt atgctccaca
    17941 aacatgagta cactactctc actgatcggg gttttcaggc acacaaataa taagtttatt
    18001 cgcattgcga agcaaacagg ggccatcctg ttgccgattt ttcatccaat gatgctttca
    18061 ccaggcggca ctaacccgga cgtagactgt attacaaaag cggcaaaaag cagagacaaa
    18121 aaacccccgc tttgcagcga gggttggaaa tttggtggag ctaagcggga tcgaaccgct
    18181 gacctcttgc atgccatgca agcgctctcc cagctgagct atagccccac gatgcgttta
    18241 cgtaccaaat ttgctgggtg caaaatttgg tggagctaag cgggatcgaa ccgctgacct
    18301 cttgcatgcc atgcaagcgc tctcccagct gagctatagc cccgtcacgt aaagcttgtc
    18361 gagttgacgg gcggcatcat atgaattccg cccgaatgtg tcaacggcaa attgcaacgt
    18421 gtagtttcaa tcgctgaaaa atcaggcaaa tgaacaattt tggaaaaccg ctcgcattca
    18481 gtagttattc atgtcacggt ttcctgtaaa gtggtgctat aaaatgaact actaatagac
    18541 ccacatacat tcagggaatt gttatgttca aggagaggat gacaccagat gaacttgcca
    18601 gactgaccgg ctatagccgc cagaccatta ataaatgggt acgcaaggaa ggctggacaa
    18661 cgtcaccaaa acctggcgtc cagggtggca aagcccgact ggttcacgtc aatgaacagg
    18721 ttcgtgaata tattcgtaat gctgaacgtc cagaaggtca gggagaagcg cctgcccttt
    18781 ctggtgatgc gccgcttgaa gtattgctgg tgacactggc gaaagagatg acgccagttg
    18841 aacaaaaaca gtttacatcc ttgcttctgc gggaagggat tatcggattg ttacaacgct
    18901 tagggattcg cgatagcaaa taatatgaaa agattacgca ataaaatgac caccgaagaa
    18961 ctggctgaat gcctcggtgt ggctaaacaa actgttaacc gttggatcag agaaaaaggc
    19021 tggaaaacgg aaaaatttcc cggcgtgaaa ggcggtcgtg ccagacttat tctggtcgat
    19081 acgcaagttt gcgagtttat tcagaacacg ccagccttcc ataacacacc gatgcttctg
    19141 gaagccgaag aaccccttgc cgaatacgca ccggggattc gcactcctgc ttatcggcaa
    19201 atcatcagtg caatagacaa catgactcac agtgagcagg aaaaagttgc gcaatttttg
    19261 tcacgcgaag gaattcgtaa tttccttgct cgtctcgaca tagatgaatc agcttgacat
    19321 cctccacgcc ctgaatgacg aggatccctg ctacgttcag gctgtcgcct gaatcatttc
    19381 ggtgggttcc tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc
    19441 gtgccccgcc gctaaaatgt tacgagcgcc gtttacatcg gcgttcgctg tatatccaca
    19501 tacctggcat ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca
    19561 cgcgcaacgc tggcttgtgt acgctggcgg aacagccagt acctgaccgc cacgccagag
    19621 ctgcttgtac tcaagctggc ggcgcatttc ataccagccc tgatccagta tcgaacggtt
    19681 taaacctgat tttgcccgga cattgcgccc cggctggctg accgtacccg ctgctgactt
    19741 tgacatgttg ctgaccttca aatcctcaat gactatcatt gcgtggtttt tgctgacggt
    19801 cgttgtgact ttgtgaaggt agtccctgcg gatatttgcg gtacaggaat gcagtcgctg
    19861 tattttgcgt ttctgcttct gccagttatt gctgaatttg accttgcggc ttaactgtcg
    19921 ctgaagtctc gccaacttct tctggttttt ctgaaaactg tttacaggct caaagactgt
    19981 gccatctgac agcgtggcga gtttagccac gccagcatcc agtccgacca ttgatgctga
    20041 agggtgaacc ggagttgata cttcactttc tgtctgaata ctgatgtacc acttaccgca
    20101 ggactggctg acagtgacat ttttcacaac acccgtgacc tgacggctgt tcaggtagcg
    20161 catccagcca agtttcggca gaaaaatacg gctgttttcc tgatcgagct taacgccctg
    20221 cgggtagcgg aatgcatcat tctgtccccg ctttttgaat cggggaaaag cagcccgctt
    20281 ccggaagaag tttttgtaag cccgctcaag ctctttcagt gactgttgca atggctgtga
    20341 cggggcatct ttaagccatt gcgtttcagt ggc
//
LOCUS       DAZFBR010000085        19850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFBR010000085 DAZFBR010000000
VERSION     DAZFBR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.085"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            161..838
                     /gene="aroM"
                     /locus_tag="ACHJZ2_003400"
     CDS             161..838
                     /gene="aroM"
                     /locus_tag="ACHJZ2_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO4569451.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            910..1194
                     /gene="ppnP"
                     /locus_tag="ACHJZ2_003401"
     CDS             910..1194
                     /gene="ppnP"
                     /locus_tag="ACHJZ2_003401"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO4569452.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            1338..3617
                     /locus_tag="ACHJZ2_003402"
     CDS             1338..3617
                     /locus_tag="ACHJZ2_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFO4569453.1"
                     /translation="MNRINILVICMVLFFMTGNACATEWISSEDLITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGDLSLPANFSNKNKLVVNGNLTISGDYDDYL
                     SGNGHLIVLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVS
                     DKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPD
                     YDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTE
                     SLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARGLVKNSNISADV
                     NKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVAD
                     ELINNEDPELRERITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTE
                     DIERIAEQMYLDNKENKNIVKVLLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISY
                     LLTQHDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNG
                     VVDEEMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVV
                     SMKNSTRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRN
                     AHTPASLLTTLTESQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDKPDLSQLRDLVK
                     TGATRKIRNEARHRLEEKQ"
     gene            3614..4063
                     /locus_tag="ACHJZ2_003403"
     CDS             3614..4063
                     /locus_tag="ACHJZ2_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569454.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIGTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(4148..5059)
                     /gene="rdgC"
                     /locus_tag="ACHJZ2_003404"
     CDS             complement(4148..5059)
                     /gene="rdgC"
                     /locus_tag="ACHJZ2_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO4569455.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            5184..6092
                     /gene="mak"
                     /locus_tag="ACHJZ2_003405"
     CDS             5184..6092
                     /gene="mak"
                     /locus_tag="ACHJZ2_003405"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO4569456.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(6235..7419)
                     /gene="araJ"
                     /locus_tag="ACHJZ2_003406"
     CDS             complement(6235..7419)
                     /gene="araJ"
                     /locus_tag="ACHJZ2_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO4569457.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(7545..10688)
                     /gene="sbcC"
                     /locus_tag="ACHJZ2_003407"
     CDS             complement(7545..10688)
                     /gene="sbcC"
                     /locus_tag="ACHJZ2_003407"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO4569458.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(10685..11887)
                     /gene="sbcD"
                     /locus_tag="ACHJZ2_003408"
     CDS             complement(10685..11887)
                     /gene="sbcD"
                     /locus_tag="ACHJZ2_003408"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO4569459.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            12077..12766
                     /gene="phoB"
                     /locus_tag="ACHJZ2_003409"
     CDS             12077..12766
                     /gene="phoB"
                     /locus_tag="ACHJZ2_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO4569460.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            12824..14119
                     /gene="phoR"
                     /locus_tag="ACHJZ2_003410"
     CDS             12824..14119
                     /gene="phoR"
                     /locus_tag="ACHJZ2_003410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO4569461.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            14526..15845
                     /gene="brnQ"
                     /locus_tag="ACHJZ2_003411"
     CDS             14526..15845
                     /gene="brnQ"
                     /locus_tag="ACHJZ2_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO4569462.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            15921..17294
                     /gene="proY"
                     /locus_tag="ACHJZ2_003412"
     CDS             15921..17294
                     /gene="proY"
                     /locus_tag="ACHJZ2_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO4569463.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            17450..19267
                     /gene="malZ"
                     /locus_tag="ACHJZ2_003413"
     CDS             17450..19267
                     /gene="malZ"
                     /locus_tag="ACHJZ2_003413"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO4569464.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            19455..>19850
                     /locus_tag="ACHJZ2_003414"
     CDS             19455..>19850
                     /locus_tag="ACHJZ2_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569465.1"
                     /translation="MTISNSNLLKPFITGTELPPFKEDVFSKTEEQFKKSTYGKSLFI
                     SGNENLVNISAEKIDEIKNSKNFFSNIVNIIILLIKHPDKILPILHGCPVESIEVIKN
                     AKRTGQWSYEMTDKNGQSIEVSYIFTDTID"
BASE COUNT         4692 a         4819 c         5228 g         5111 t
ORIGIN      
        1 acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt gcacgtctgc
       61 tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct taacacttgc
      121 ccggtaatta acgacgaaat aaaagtaagg tggaagaaca atgagtgcgt cgttggcgat
      181 tctaaccatc ggcattgtac ctatgcagga agttttgccg ctcctgacag aatacattga
      241 cgaagataat atttcccatc atagcctgct ggggaagtta agccgcgaag aagtgatggc
      301 ggagtacgcg ccagaagcag gcgaagacac aattctcaca ttattaaatg acaaccatct
      361 ggcccatgtt tcgcgtcgca aagtagagcg tgacctgcaa ggtgtggttg aagtgctcga
      421 taatcagggc tacgacgtca ttatattaat gagtacggca aacattagta gtatgactgc
      481 gcgtaatacg atctttcttg agccgtcgcg gatattgcca ccgctggttt cctctattgt
      541 tgaagatcat caggtggggg ttatcgtacc ggttgaggag ttgctgaccg ttcaggcgca
      601 aaaatggcaa attttgcaga aaccgcctgt attttcattg ggtaacccca ttcatgattc
      661 agaacaaaaa atcattgatg ccgggaaaga attactggcg aaaggcgcag atgtcatcat
      721 gctggattgt ttgggattta accagcgtca tcgcgattta ctgcaaaaac agctcgatgt
      781 tcctgtcttg ctctctaacg tattgattgc acggctggct gcggaattac tggtgtaatt
      841 ttgcgtgaca gccagcgcat ctggccccta tagtgaagta gatattcaac taccagacag
      901 ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc gatcggcttt
      961 tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga atacaccttc
     1021 agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt gttactgcct
     1081 gacgcgaccg actggcaagt gtatgaagcc ggttcggtgt ttaatgttcc cggtcacagt
     1141 gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct gtaattcctc
     1201 gccttccgct tgaacgggag ggcatttttc tgaaatgtcc tctcataacc ccataataat
     1261 atttcctttg ctgcgatttt ttcaatttcc gatatattca taattcatca aggttgatat
     1321 aaatatcatt gaagattatg aatagaatta atatattggt catctgtatg gtcttatttt
     1381 ttatgactgg caatgcatgt gccacagagt ggatcagtag cgaggacctt atcacctctg
     1441 actttcatct tatgacggca gatgagcgca acgtggttaa agcggcaacc gacgatagta
     1501 tggaagctgc ttatatgcta aaagataata tccgctggta ttatcataat ggtgacctat
     1561 cattacccgc taacttcagc aataaaaata aactggtggt gaacggtaat ttaacgattt
     1621 ccggcgatta tgatgattat ctgtcaggta atggtcatct gattgtatta ggtaatgtta
     1681 ttgtcgataa ctttattaat catgatttcg cgtatgtcaa aggacagatg acagcgaaag
     1741 gtttagttta tgctgattac aatgatcata actttgaagt gatgaaggga attagtgcgc
     1801 gaggcattat tgtttccgat aaagcaacgc aatttgaagt cattaaagct gaattttata
     1861 tcaatgaaga tggaagtggt gaaggatata actgggatga gaatattcaa aaagcctatt
     1921 cactggtgac tgccgatctt tatgatcaca ctgaaatcga aaccgataac atttccaatg
     1981 cgtatcctga ctatgatagc gtcgcagaca acatcgttca gggtttaccg ctttttcgag
     2041 ataaagccgc tcctgaaatt aatgagaaat taaaatggat tgaaacgggg aaactcgata
     2101 atttcccagc gaataaaata aagcatcaag atccactggt ggcacgtttt ctgactcata
     2161 cagagagttt atcacccgcc gttatgctgc aactgttaca gcaccccgat gaccagacgc
     2221 gtgaatcgat ggcgcagtcc tggcccgctc aacagatgca ttggcttacc gatgaattaa
     2281 ttaaagatga ggctgttgcc agagggctgg ttaaaaacag taatatttcc gccgatgtga
     2341 ataaaaaatt aatgtctgta cccgtcgaga gcgtacagct ggagcaggcc agacaggata
     2401 atctttctcc agatattgtt gcgtcgttaa gtcatagccc ctttctcagt gtacgcaaga
     2461 cattactcag ccattacgat tacgcctggc tggttccgac tgcagtggcc gatgagttga
     2521 taaataacga agacccggag ttacgtgaaa gaattaccgg agcagattta accgcacaac
     2581 aagcagtgat gttgagtaaa gataaatcac tgaaagttcg tgaagcatta gcccggacgc
     2641 ttactgaatt aaaaattacc caactgtcag caaccctgcg tacagaggat attgaacgta
     2701 tcgcagaaca aatgtatctg gataataaag aaaataaaaa tatcgtaaag gtgttattga
     2761 tagcgttgcc ggagatgcgt caactgtctc tggcaaaaga agatgtgcac aatttgcgcg
     2821 aaggtgcgcg ttatctgaca tcgaaggacg tgattagtta tctattgact cagcatgatg
     2881 ttcccaccgt ctgggatgag ctggcacgcg ataaattatt gccgctggaa tataagaaac
     2941 agttatggca acgcacatta aatttaatga tgagcaaacg gcaagaagat caagagcaag
     3001 cctacgaagt acagctggcg ctgattgata atggtgttgt tgacgaagag atgttaaata
     3061 atgccattga tttactggtt gatttacccg ctgaataccg ctatcgaatg cgtaaccaat
     3121 tatttgataa taaagaatta ccttccggaa ttatcaacaa actcgatcaa cagtatcgct
     3181 tcaactctga ctgggcgctg gctgttgtca gtatgaaaaa tagcacccgt agacaaagtg
     3241 aaagaggatt acaccgctgg aaccatgagg atagcgatat ttttgcggaa ctggcgacga
     3301 taaaagataa atcagatgat gaatggtggc gtgcattgct gcaaagtcgc aatgatcatt
     3361 tgcgccaaac ggcattacgc aacgcacata ccccggcgtc gttgttaacg acattgactg
     3421 agtctcagga tcggtcgctg gctattaata atccacagct ggctgccgat gtgaaaacgg
     3481 tgtggctaaa agaggatcca tcattacttt tatttgtcga taaacccgat ctttcgcagt
     3541 tacgggattt agtgaaaacc ggggcaacgc ggaaaattcg caatgaagcg cgtcaccggc
     3601 ttgaggaaaa acaatgacac aacgcccctg gagcaagtta cagcgggaga tctatgactt
     3661 actgacgcca acgatcaacc tgcaaattca ctgtacccgt tatcccatgc gcagccaaaa
     3721 tggtggaagc accgatttac cgcgttattg gatcacgttg gataaaaacg tgatctggga
     3781 ttacccgaaa gactttattg caggcaacgg tggagtgcgt aattttcacg gtgaaacttg
     3841 ctggtatcct tacctgacgg atatttgttc aatttctgat ttattgcgtg aatatattgg
     3901 caccccaaaa gcggagttgt taacaaaaca atttaccagc gataaatggg ggttagtgaa
     3961 tattctgcgt gctgctgaca ggcgaatagg gatgaggcga ttagatcagt tacggcgtaa
     4021 aacgcataat attgctgcgc taaaaaatta ttgcccgccg tagcgaataa ttatatgcct
     4081 ggtgtggctt cgtacgccgg ataagacgcg gcaggcgtcg catccggcat taaaggaaaa
     4141 tcagcaatta acgttgtgct tcgccaccta atccttcaat caggttttga attaacgctg
     4201 ccagttcacc agtcatcagg atgaaatcgg catcaaaacg ctgggcgaaa tcttcacggt
     4261 cgatatcttc gttttgatcg cgcagctcgt cgcagaactt cagacgcttg agcgaaccat
     4321 cgtcgcacat cacaaactga atgcgctgct gccagtcgag cgccagttta gtcaccactt
     4381 ttccggcttc aatgtgattg gtgatctctt cgctggtcag atcttgtttc ttcgcgcgga
     4441 tcactccgcc atcttccagc aacgatttca gctcggcttc atcaagcagc tggaagccct
     4501 gtgccgcact accggaacgt acccattcgg tcagcgtcag ttcaatcggg tttgccatgc
     4561 tcaatggtac aaccggtaac gaccccaggc ttttacgcag cagtgccagc gtatcttccg
     4621 cttttttggc actggcgcag tccaccataa tcaaaccgtt aaccgtgtcg atccacatca
     4681 ttgtctggct aaaacggctg aaagcacgcg gcaacagaga gtgcagcact tcgtctttca
     4741 gcgaatcttt ttcggttttc ttcagcttac gcgcctgttc cgcttccagt ttggcgattt
     4801 tcgcttccag cgcctgttta atcaccggag acggtaggat tttttcttct ttgcgcgcgc
     4861 agataacaat ttgaccattg gcaacgtgcg ttaacgcatc gctgtgcgat cccatcggag
     4921 gaacccagcc catcttcgcc atgtcctggc tgccgcatgg ggtaaatgcc atcgaggcta
     4981 gctgtttttc catctcttct gcacgcagcg aaatctcgcg gctaagacgg taaaccatta
     5041 aatttttgaa ccacagcatg ataatttcca cggccttgtc gttaaattta gcgggcatga
     5101 taacgaattg tcggcggcct tgcattgcca atccggttgt ccgtctctac gctattgata
     5161 ttgaaaaaaa taaggagagt accgtgcgta taggtatcga tttaggcggc accaaaactg
     5221 aagtgattgc actgggcgat gcaggggagc agttgtaccg ccatcgtctg cccacgccgc
     5281 gtgatgatta ccggcagact attgaaacga tcgccacgtt ggttgatatg gcggagcagg
     5341 cgacggggca gcgcggaacg gtaggtatgg gcattcctgg ctcaatttcg ccttacaccg
     5401 gtgtggtgaa gaatgccaat tcaacctggc tcaacggtca gccattcgat aaagacttaa
     5461 gcgcgaggtt gcagcgggaa gtgcggctgg caaatgacgc caactgtctg gcggtttcag
     5521 aagcagtaga tggcgcggca gcgggagcgc agacggtatt tgccgtgatt atcggcacgg
     5581 gatgcggcgc gggcgtggca ttcaatgggc gggcgcatat cggcggcaat ggcacggcag
     5641 gtgagtgggg acacaatccg ctaccgtgga tggacgaaga cgaactgcgt tatcgcgagg
     5701 aagtcccttg ttattgcggt aaacaaggtt gtattgaaac ctttatttcg ggcacgggat
     5761 tcgcgacgga ttatcgtcgt ttgagcggac atgcgctgaa aggcagtgaa attatccgcc
     5821 tggttgaaga aagcgatccg gtagcggaac tggcattgcg tcgctacgag ctgcggctgg
     5881 caaaatcgct ggcacatgtc gtgaatattc tcgatccgga tgtgattgtc ctggggggcg
     5941 ggatgagcaa tgtagaccgt ttatatcaaa cggttccgca gttgattaaa caatttgtct
     6001 tcggcggcga atgtgaaacg ccggtgcgta aggcgaagca cggtgattcc agcggcgtac
     6061 gcggcgctgc gtggttatgg ccacaagagt aaaaaaccca ggcaattggc gcatcatgcc
     6121 tgatgcgacg cttgccgcgt cttatcaggc ctacaaaatg tgccagaacc gcgtagggcg
     6181 gataaggcgt tcacgccgca tccggcaata agcaacgcac accgtgacag taatctaccc
     6241 cagtggtttc gccagcaccg gactatccgc cgcttgctgg cgcttatagc gaccatacag
     6301 cagcaacgat gacatcgcag caaacgaaag cagggcggca ggcagcgcca cgtaattata
     6361 tgccagcccc agcgtcagca tcatacctcc gcaatatgcg ccgacggcgc taccgaggtt
     6421 aaacgctatt tgcccacctg cggcacctaa taactctccg cctttggcat tttgcagtaa
     6481 caatatttgc agaggtgctg aaagggcaaa taatcccgcg caacaaataa aagcaaaaat
     6541 aagcgacgtt gttttcatgc cgccgcagaa aaagagcatc agcagtgcca gtacaattat
     6601 aaagtcagtc actgctgcaa tgcgcagtgg tgaataacgt cctgaaatcc tgccacttag
     6661 catatttccc agcaccatcc ctagcccaac taacatcata ataaaggtca tcgccgtttc
     6721 cgaaaaaccg gaaataaaca tcatgtatgg ctttacgtag ctgaaccagg caaacacacc
     6781 tgcgttgcca aacatggtgg cggcgaaaat taaccacggg gccgggctgc gcaaaaagtg
     6841 aaattgttcg cgcagctttc ctttcgcctc gtcgcgaata tccggcaccc aaaaatagac
     6901 cgatgccatc accgcaatat taaaaacagc gatcaataaa aaggtgtaac gccagctaaa
     6961 ttcctgactt aaatacgttc ccagcggaat gcccagcaaa ttggcgactg tcatcccgga
     7021 aaccatcccc gccacggcgg cggtgacttt tccgggtttg ataatttttg ataacacgat
     7081 cgctccgacg ccaaaaaatg cgccatgcgg aaagccggaa accaggcgac caatggcaag
     7141 catcaggtac gacgaagaga gcgtgaacat ggcgttgcca atgacgcaca acgccaccag
     7201 aaacaacaag atatgtttga gtgagtagcg gctggaaaag agtgcgatta ttggcgcacc
     7261 gaccaccacc cccagcgcat aatacgagat catatgcccg gcagcaggaa tcgaaattcc
     7321 tacgttatgc gccagctccg tgagcacgcc cataatgcca aattcggcca tccccaaacc
     7381 aaacgtgccc agagccaaag ataaaatgac ttttttcata ccaccatcac aactaccaga
     7441 acggctacca atcaccgtag ccaataaagt gatagcttgc aggataacga ccagcaaggc
     7501 caagagataa ttcgcccctc tgtattcatt atcctgctga atagttattt cactgcaaac
     7561 gtactttcca gtttgctgta gcccaggccg ttgatcttct tcactttgat ctgcacagga
     7621 atacgctctt tcatcgcttc aacgtggcta atcacaccga tggttttgcc actggcgttc
     7681 agggcatcca gcgcatcaag ggcggtatcc agcgtttcgc tatccagcgt gccaaaacct
     7741 tcatcaagga acagcgagtc aatacgtgtt ttatggctga ccagatccga aagcgccagc
     7801 gccagcgcca gactaacgag gaaactttcg ccgccggaaa gggtacgggt atcgcgtacc
     7861 gcatctgcct gccaggtatc aacaacctcg acttccagcg cctcgctggc tttgcgctgt
     7921 aacagatagc gcccgtgcag ccgggtaagt tgctgattag cgagatggac taaattatcc
     7981 agcgtcagcc cctgggcaaa cttgcggaat ttatcgccct ctttggaacc tattagcgaa
     8041 ttcagatatc cccagtcctc aacctgctgc gtcatttgag caatttgctg cagtaaggtt
     8101 tgttgttgct gacggttatc tgcatcctgc ttcagctgct ggcgaatctc gccttgactc
     8161 gtggtgtttt cacgcaactt ttggtgagtt tgcgctaact cttgctgaat ctgctccacc
     8221 gtcacagtga gagccaaccc gtcaggtcgg tgttgttgat gctgtgccag cgtttctgct
     8281 gtctgagtga ccagcgtttg cgcctgacgg cgctggtttt ccagattctg cttgagctgt
     8341 tccagctgcg ttagtgtttg ttcatccatt agcgccgcaa ggaacgcctg ctgatcgtca
     8401 aagacgctgg cctgtagcgc ggtgtcaaac tgcgcctggg ctttttgcag actttgcgcc
     8461 gccagaacat cctgttgctg taacgtctgc tgctggctgt gtaatgcgag acattgttca
     8521 tgaacctgcc gccagttatc cagcgccaca gtttcttcgc tgtgcgggag atcatcactt
     8581 tgcggcaacg tttccagaat cggcgtcagc tgctgaatac ggttttgcag cgcggttaat
     8641 tcgttctggc gttgctgcca gctctgcgct tcttgctgac gtgtcgccaa ccagctctct
     8701 tcttcatctt cctgtggcaa tgtcagtgca taacccgcca atgccgttaa gagctgctgc
     8761 tggcgttgtt caatttgctg ttgatactgg ataatttgct gattatgcgc ggcaatctgc
     8821 ccttgtaatt catgtcgttg gctgagtaac cgcagctggc gttcgtgctc atcttgtgca
     8881 tccagccacg gttgaatatc gtcctgtggc tgcaaggtga tattgaggct ggccgtgacg
     8941 gcttgccatt gttgagtaag tgcttgctca tcttgtcgga ggctttgcgc ttcgttttca
     9001 tcgcgctgaa gctgcttcgt taatgcatcc agttgcccgc gcagcgccgc accttcttcg
     9061 ccgagctttt taacttcgtt ttccagcgcc agtaatcgag actgattaac gccaggctcc
     9121 agcgcctgat acgcctcgac cgccggatgg ctggtggaac cacaaagtgg acaaggctga
     9181 cccgcctgta actgtgcacg ttgagcttcc agcgttttga tgcgcgcttc ctgctcgcaa
     9241 atggttttca catcggcaag ttgctgcgtc ttttctttat aacgctggcg catttcgtta
     9301 agtgcggcat tacgttgcgt ctgttccaga gtgacattct ggatagtgac cattaactgc
     9361 gccagacgtt tttgttgggg aacaatctgc ccatgcagcg cgaccaggcg ctgacgcagt
     9421 gggcgttgct cagcatgttg cgccagggcg gtagcaactt catcggcggt taacatcaac
     9481 gtgatcgccg caagcgcatt aagtttttgc tcagcatggg ttaactgttg ctgccattgc
     9541 cgcagatgct cgcgatcgct ggtttgttgg gagaactgcg cacgccaacc cgccagttcg
     9601 ttgttccact gacggaagcg gtcgtgttcc tgtaaccagg tattcaggct ttgttgctgc
     9661 tgctgtaatt ctgctgactg cttcgccgcg tggtggcgaa tgctcgcgcg aagcgccatt
     9721 gtgttctgta agcgagtatt tacttcttca atctgctggc gagtatgcgc cagcgccgcg
     9781 ctgtgttctg cgatgcgttc ccagtgcgga cgaagatttc gtgccggttg tgccagactg
     9841 agcgccgcca gttgaggttg cgctttttct tcttcggcta acgcctgttg caaggcctgc
     9901 tgacggcggc tggcttcttg ctgcaattcg tccagacgcg ttaaccagtt tagtgattgt
     9961 tgttcttgct gctgcgcggt aagtaactgt ttttcttcgt cagtaagtac ctgcaaactc
    10021 gctgtcagcg attgcacttg ttccggcgtg agcaacgcga cgccgctggc ctgcgcttgc
    10081 agcttctcca gctctgtgcg ggccgatttg tgctgctcaa aaaccatcgc cgagatttgc
    10141 ccgtagattt cagtgccggt taactcctcg agcaattccg cgcgttcttt gggtttggca
    10201 ttcaggaagg cagcaaattg cccctgcgaa agcagcatcg aacgggtgaa gcgcccgtaa
    10261 tccagcccgg ttaacgtcgc tgtcagttcc agcttatctt tcactttgtc ggcgagaatt
    10321 ttgccgtcgg cgcagcgcgc cagctctacg cgtggcacct gcaaattacc gtcgggttgg
    10381 ttacgcgccc gattctggct ccagaacgcg cgatacgctt cacctttcac ttcaaactcc
    10441 acctccgcca gacattcagc ggtatcgcgg gtcatgagat cattttgcga ttgtgaaacg
    10501 ttagagagac gcggagtttc gtgatacagc gccagacaaa tggcgtccag cagggtggtt
    10561 ttccccgcac cggtcgggcc ggtaatggca aacagcccgt tgctggcgaa cggctcgcgg
    10621 gtgaaatcaa tcttccattc gccttttaat gagttcaggt ttttcaggcg caggctgaga
    10681 attttcatgc ttcgtgttct ccggcgaggg tatgcaacgt cgtggtgaaa agatgctgca
    10741 gacgttgctg ctgcgattca tccagttctt ccagtgccag acggcgattg aacacctctt
    10801 cgacgctgag ttcgctgagg gtttcacgct gttggctggc taacacacgc tcgcgctgtt
    10861 cacgactccg acgtaccagc aatacttcaa caggcaatga ttcggttaat gcctggattt
    10921 tgcgctgaat atcatgcaga tactcatcag tagtgatttc gatatccagc cagacaggtg
    10981 gctcctgcga tacatcgcgc cactgttcca gctgtgcggt aatcgacgcc agatcgcctt
    11041 tcagcactgc catgggttgc gttaccggta cgttcaggtt ttccacgctc tctaatttgc
    11101 cgtttgaaaa tgtcaccaga tggacatatt tactcttacc gcattcatca aaactcagtg
    11161 gaatggggga gccgcaatag cgaacatgtt ccatgccgcc aataatctgt gcgcggtgaa
    11221 tatgcccgag cgcgatgtag tcggctggtg gaaagttttg tgccggaaac gcgtccagcg
    11281 tgccaatata aatgtcacgc acggcgtcac ttttactggc ccccacggtc gttaaatgtc
    11341 ccgtggcgat gatgggcaga ggctgatcgc cgcgcagttt gcaggcatcg gcatagtgtt
    11401 gttggtaata atcggtaatc gctgccagta aatgctgctg tttttcaata ccgttaagcc
    11461 ccgcctggct ggtaataatg tcacgcggac gtaaaaacgg aatggggcac agcactgcgc
    11521 ctggcgtccc gtcgcgacga ggcaagattt gcggcgcatg tccggcgctg gcgaccacgg
    11581 tagtattgag gaacgccatg atatcgcgcg attcattcag cgtggcgacc gagtcatggt
    11641 ttcctgccag taccaccaga tgacagccag tttgctgtaa attgacaaca aaacggttgt
    11701 ataacgtgcg ggcgtaactg ggcggcgagc cggtatcgaa aacatcaccg gcaacaataa
    11761 tcgcatccac ctgatgggtt tgtgctgtct ccagcagcca gtcaagaaaa gcctgatgtt
    11821 cagcttcgcg gcttttactg tagaagttct ggccgagatg ccagtctgag gtgtgaagga
    11881 tgcgcataac ggttccctgg cgaaaaagca tgggcgcgat tatacccaaa cagatgtgcc
    11941 atttgctttt ttctgcgcca cggaaatcaa taacctgaag atatgtgcga cgagcttttc
    12001 ataaatctgt cataaatctg acgcataatg acgtcgcatt aatgatcgca acctatttat
    12061 tacaacaggg caaatcatgg cgagacgtat tctggtcgta gaagatgaag ctccaattcg
    12121 cgaaatggtc tgcttcgtgc tcgaacaaaa tggctttcag ccggtcgaag cggaagatta
    12181 tgacagtgct gtgaatcaac tgaatgaacc ctggccggat ttaattctcc ttgactggat
    12241 gttacctggc ggctccggta tccagttcat caaacacctc aagcgcgagt cgatgacccg
    12301 ggatattcca gtggtgatgt tgaccgccag aggggaagaa gaagatcgcg tgcgcggcct
    12361 tgaaaccggc gcggatgact atatcaccaa gccgttttcg ccgaaggagc tggtggcgcg
    12421 aatcaaagcg gtaatgcgcc gtatttcgcc aatggcggtg gaagaggtga ttgagatgca
    12481 gggattaagt ctcgacccga catctcaccg agtgatggcg ggcgaagagc cgctggagat
    12541 ggggccgaca gaatttaaac tgctgcactt ttttatgacg catcctgagc gcgtgtacag
    12601 ccgcgagcag ctgttaaacc acgtctgggg aactaacgtg tatgtggaag accgcacggt
    12661 cgatgtccac attcgtcgcc tgcgtaaagc actggagccc ggcgggcatg accgcatggt
    12721 gcagaccgtg cgcggtacag gatatcgttt ttcaacccgc ttttaacgcc ttgctcatcg
    12781 gacgcagagc agggcttatg atttcttaac tggagtatct tacgtgctgg aacggctgtc
    12841 gtggaaaagg ctggtgctgg agctgctact ttgctgcctc ccggctttca tcctgggtgc
    12901 attttttggt tacctgccct ggtttttgct ggcatcggta acaggactgc ttatctggca
    12961 tttctggaat ttattgcgcc tttcatggtg gctgtgggtg gatcgcagta tgaccccgcc
    13021 accggggcgt ggtagctggg aaccgctact atacggctta caccagatgc agctgcgaaa
    13081 taaaaaacgc cgccgtgaac tgggcaatct gattaaacgc tttcgtagcg gcgcggagtc
    13141 gctgcccgac gcggtggtgc tgaccacgga agagggcggt attttctggt gtaacggtct
    13201 ggcgcaacag attcttggtt tgcgctggcc ggaagataac gggcagaaca tccttaacct
    13261 gctgcgttac ccggagttta cgcaatattt gaaaacgcgt gatttttctc gcccgctcaa
    13321 tctggtgctc aacaccgggc ggcatctgga aattcgcgtc atgccttata cccacaaaca
    13381 gttgctgatg gtggcgcgtg atgtcacgca aatgcatcaa ctggaagggg cgcggcgtaa
    13441 cttttttgcc aacgtgagcc atgagttacg tacgccattg accgtgttac agggttacct
    13501 ggagatgatg gatgagcagc cgctggaagg cgcggtacgc gaaaaagcgt tgcacaccat
    13561 gcgcgagcag acccagcgga tggaagggct ggtgaagcag ttgctaacgc tgtcgaaaat
    13621 tgaagccgca ccgacgcaat tgctcaatga aaaggttgat gtgccgatga tgctgcgcgt
    13681 tgttgagcgc gaggctcaga ctctgagtca gaaaaaacag acatttacct tcgagataga
    13741 taacggcctc aaggtgtctg gcaacgaaga tcagctacgc agtgtgattt cgaacctggt
    13801 ctataacgcc gtgaatcata cgccggaagg cacgcatatc accgtacgct ggcagcgagt
    13861 gccgcacggt gccgaattta gcgttgaaga taacggaccg ggcattgcac cggagcatat
    13921 tccgcgcctg accgagcgtt tttatcgcgt tgataaagcg cgttcccggc aaaccggcgg
    13981 tagcggatta gggttagcga tcgtgaaaca tgcggtgaat catcacgaaa gtcgcctgaa
    14041 tattgagagt acagtaggaa aaggaacacg tttcagtttt gttatcccgg aacgtttaat
    14101 tgtcaaaaac agcgattaat ccgcctttgt catcttttat tgccataagc cagccgatgc
    14161 tggcttattt tctttgcagt caaaatacgg gcgttagatt ttacaacggt tggtgatttt
    14221 ttgttcgcat gattagccat gtctttttca cggaaatagt gttttatact ggttggtgat
    14281 ttcttatcgc tatatacctc tggtttttag atccctcctt gctttaaaac gttataagcg
    14341 tttaaattgc gcttcaggtg ctgtcatact gactgcatta acgcggtaaa tcgaaaaact
    14401 attcttcgcc gcgcctggtt gggagtattt cccgctaaaa ttgtttaaat ataccgctgt
    14461 atcatcccca gggattggca caaaaattta acgttacaac accacatcca caggcagtat
    14521 gatttatgac ccatcaatta agatcgcgcg atatcatcgc tctgggcttt atgacatttg
    14581 cgttgttcgt cggcgcaggt aacattattt tccctccaat ggtcggctta caggcaggcg
    14641 aacacgtctg gactgcggca ttcggcttcc tcattactgc cgttggcctg ccggtattaa
    14701 cggtagtggc gctggcaaaa gttggcggcg gtgttgacag cctcagcacg ccaatcggta
    14761 aagtcgctgg cgtactgctg gcaacggttt gttacctggc ggtggggccg cttttcgcta
    14821 cgccgcgtac agctaccgtt tcttttgaag tggggattgc gccgctgacg ggtgattccg
    14881 cgctgccgct gtttatctac agcctggtct atttcgctat cgttattctg gtttcgctct
    14941 atccgggcaa gctgctggat accgtgggca acttccttgc gccgctgaaa attatcgcgc
    15001 tggtcatcct gtctgttgcc gcgattgtct ggccggcggg ttctatcagc acggcgactg
    15061 aggcttatca aaacgctgcg ttttctaacg gcttcgtcaa cggctatctg accatggata
    15121 cgctgggcgc aatggtgttt ggtatcgtta ttgttaacgc ggcgcgttct cgtggcgtta
    15181 ccgaagcgcg cctactgacc cgttataccg tctgggctgg cctgatggcg ggtgttggtc
    15241 tgactctgct gtacctggcg ctgttccgtc tgggttcaga cagcgcgtcg ctggtcgatc
    15301 agtctgcaaa cggcgctgct attctgcatg cttacgttca gcacaccttt ggcggcggcg
    15361 gtagcttcct gctggcggcg ttaatcttca tcgcctgcct ggtaacggca gttggcctga
    15421 cctgtgcttg tgcagaattc tttgcccagt acgtaccgct ctcttatcgt acgctggtgt
    15481 ttatcctcgg cggcttctcg atggtggttt ctaacctcgg cttaagccag ctgatccaga
    15541 tctccgtacc ggtgctgacc gctatttatc cgccgtgtat cgcactggtt gtattaagtt
    15601 ttacacgctc atggtggcat aattcgtccc gcgtgattgc tccgccgatg tttatcagcc
    15661 tgctttttgg tattctcgac gggatcaaag catctgcatt cagcgatatc ttaccgtcct
    15721 gggcgcagcg tttaccgctg gccgaacaag gtctggcgtg gttaatgcca acagtggtga
    15781 tggtggttct ggccattatc tgggatcgcg cggcaggtcg tcaggtgacc tccagcgctc
    15841 actaaatcac tgaacatttg ttttaaccac ggggctgcga tgccccgtgg ttttttattg
    15901 tgttgatggg ttaggaattg atggaaagta agaacaagct aaagcgtggg ctaagtaccc
    15961 gccacatacg ctttatggca ctgggttcag caattggcac cgggctgttt tacggttcgg
    16021 cagacgccat caaaatggcc ggtccgagcg tgttgttggc ctatattatc ggtggtatcg
    16081 cggcgtatat cattatgcgt gcgctggggg aaatgtcggt acataacccg gccgccagct
    16141 ctttctcgcg ttatgcgcag gaaaacctcg gcccgctggc aggttacatt accggctgga
    16201 cctactgctt tgaaatcctt attgtcgcca tcgccgatgt gaccgctttt ggtatctata
    16261 tgggtgtctg gttcccgacg gtgccgcact ggatttgggt actgagcgtg gtgctgatca
    16321 tttgcgccgt aaacctgatg agcgtgaagg tattcggtga gctggaattc tggttctcgt
    16381 tctttaaagt cgccaccatc atcatcatga ttgtcgccgg tttcggcatc atcatctggg
    16441 ggattggcaa cggcgggcaa ccgaccggta ttcataacct gtggagcaac ggcggcttct
    16501 tcagtaacgg ctggcttggc atggtaatgt cgttgcaaat ggtgatgttt gcttacggtg
    16561 ggatcgaaat tatcgggatt accgccggtg aagcgaaaga tcctgagaaa tcgataccgc
    16621 gtgcgattaa ctccgtgccg atgcgtattc tggtgttcta cgtcggtacg ctgttcgtca
    16681 ttatgtctat ctacccgtgg aatcaggttg gcactgccgg tagcccgttc gtgctgacgt
    16741 tccagcatat gggcattacc tttgccgcca gcattcttaa ctttgttgtg ctgactgctt
    16801 cgctgtcggc aattaacagt gatgtatttg gcgtaggccg tatgctccac ggtatggcag
    16861 agcagggcag cgcgccgaaa attttcagca aaacgtcgcg tcgcggtatt ccgtgggtta
    16921 cggtgctggt gatgactacc gcgctgctgt ttgcggtgta tctgaactac atcatgccgg
    16981 aaaacgtctt cctggtgatc gcttcgctgg caaccttcgc cacggtgtgg gtgtggatta
    17041 tgatcctgct gtcgcaaatt gccttccgtc gccgtttgcc gccagaagaa gttaaggcgc
    17101 tgaaatttaa agtgccgggt ggggtagcaa cgaccatcgg cgggctgatt ttcctgctct
    17161 ttattatcgg gttgattggt tatcacccgg atacgcgtat ctcgctgtat gtcggtttcg
    17221 cgtggattgt tgtgctgttg attggctgga tgtttaagcg tcgccacgat cgtcagctgg
    17281 ctgaaaacca ataatacttg cgttctgtaa gccggttcag gaggaaatcc tgatccggct
    17341 attccgaaag ttatccgccc ccgtcctcct cccccaaata tccttcagat gatgagtgat
    17401 cctgcattag gctatggcaa ggtgatcaga ttttcatcac aggggaatta tgatgttaaa
    17461 tgcatggcac ctgccggtgc ccccatttgt taaacaaagc aaagatcaac tgctcattac
    17521 actgtggctg acgggcgaag acccaccgca gcgcattatg ctgcgtacag aacacgataa
    17581 cgaagaaatg tcagtaccaa tgcataagca gcgcagtcag ccgcagccag gcgtcaccgc
    17641 atggcgtgcg gcgattgatc tctccagcgg acaaccccgg cggcgttaca gtttcaaact
    17701 gctgtggcac gatcgccagc gttggtttac accgcagggc ttcagccgaa tgccgccggc
    17761 acgactggag cagtttgccg tcgatgtacc ggatatcggc ccacaatggg ctgcggatca
    17821 gattttttat cagatcttcc ctgatcgttt tgcgcgtagt cttcctcgtg aagctgaaca
    17881 ggatcatgtc tattaccatc atgcagccgg acaagagatc atcttgcgtg actgggatga
    17941 accggtcacg gcgcaggcgg gcggatcaac gttctatggc ggcgatctgg acgggataag
    18001 cgaaaaactg ccgtatctga aaaagcttgg cgtgacggcg ctgtatctca atccggtgtt
    18061 taaagctccc agcgtacata aatacgatac cgaggattat cgccatgtcg atccgcagtt
    18121 tggcggtgat ggggcgttgc tgcgcttacg ccacaatacg cagcagctgg gaatgcggct
    18181 ggtgctggat ggcgtgttta accacagtgg cgattcccat gcctggtttg acaggcacaa
    18241 tcgtggcacg gggggagctt gtcacaaccc cgaatcgccc tggcgcgact ggtactcgtt
    18301 tagtgatgat ggcacggcgc tcgactggct tggctatgcc agcttgccga agctggatta
    18361 tcagtcggaa agtctggtga atgaaattta tcgcggggaa gacagtattg tccgccactg
    18421 gctgaaagcg ccgtggaata tggacggctg gcggctggat gtggtgcata tgctggggga
    18481 agcgggtggg gcgcgcaata atttgcagca cgttgctggg atcaccgaag cggcgaaaga
    18541 aacccagccg gaagcgtata tagttggaga gcattttggc gatgcacggc aatggttaca
    18601 ggccgatgtg gaagatgccg ccatgaacta tcgcggcttc acattcccgt tgtggggatt
    18661 tcttgccaat accgacatct cttacgatcc gcagcaaatt gacgctcaaa cctgtatggc
    18721 ctggatggat aattaccgcg cagggctttc tcatcaacag caattacgta tgtttaatca
    18781 gctcgacagc cacgatactg cgcgatttaa aacgctgctc ggtcgggagg ttgcgcgcct
    18841 gcccctggca gtggtctggc tgttcacctg gcctggtgta ccgtgcattt attacggtga
    18901 tgaagtggga ctggatggca aaaatgatcc gttttgccgt aagccgttcc cctggcaggt
    18961 ggaaaaacag gatatggcgt tattcgcgtt gtaccagcga atgattgcgc tgcgtaagaa
    19021 aagtcaggcg ctgcgtcgtg gcggctgtca ggtgctgtat gcggaagata acgtggtggt
    19081 atttgtccgc gtgctgaatc agcagcgtgt actggtggca attaaccgtg gcgaagcctg
    19141 tgaagtggtg ctacccgcgt caccgtttct taatgtcgtg caatggcaac gcaaagaagg
    19201 ccatgggcaa ctgacagacg ggattctggc tttgcctgcc atttcggcta cggtgtggat
    19261 gaactaaagc atctgtttta tttgttacgt cgttagtcac cagccctgac actacgcagc
    19321 gcaaaaaagc tgctcaatgt cagggctgaa tttgttaaat atttgtcccg atcgatatga
    19381 gcgatttttg gtattgtttg cccatgatgt tatgtacaac atgctccagg ataaccctgt
    19441 agtggagcaa agatatgaca atttcaaatt cgaatttatt gaaaccgttt attactggta
    19501 ctgagttgcc accttttaaa gaggatgtct tttctaaaac agaagaacaa tttaaaaaaa
    19561 gcacctatgg caaatcgctt ttcatttccg gaaatgaaaa cttagtaaac atttcagcgg
    19621 aaaaaatcga tgaaataaaa aatagcaaaa atttcttttc aaacattgta aatataataa
    19681 ttttactcat taagcatcct gataaaattc tgcccattct tcatggttgt cctgtagagt
    19741 cgatcgaagt cattaagaat gcgaaacgaa cgggacaatg gtcatatgag atgactgata
    19801 agaacgggca gagtattgaa gtgtcctata ttttcactga taccatagat
//
LOCUS       DAZFBR010000086        19501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFBR010000086 DAZFBR010000000
VERSION     DAZFBR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.086"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            13..138
                     /gene="yahH"
                     /locus_tag="ACHJZ2_003415"
                     /pseudo
     CDS             13..138
                     /gene="yahH"
                     /locus_tag="ACHJZ2_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
     gene            188..1138
                     /gene="yahI"
                     /locus_tag="ACHJZ2_003416"
     CDS             188..1138
                     /gene="yahI"
                     /locus_tag="ACHJZ2_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO4569466.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            1148..2530
                     /gene="yahJ"
                     /locus_tag="ACHJZ2_003417"
     CDS             1148..2530
                     /gene="yahJ"
                     /locus_tag="ACHJZ2_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO4569467.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            2829..3239
                     /locus_tag="ACHJZ2_003418"
     CDS             2829..3239
                     /locus_tag="ACHJZ2_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO4569468.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            3493..4476
                     /locus_tag="ACHJZ2_003419"
     CDS             3493..4476
                     /locus_tag="ACHJZ2_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4569469.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            4525..6009
                     /locus_tag="ACHJZ2_003420"
     CDS             4525..6009
                     /locus_tag="ACHJZ2_003420"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4569470.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSPPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            6002..6973
                     /locus_tag="ACHJZ2_003421"
     CDS             6002..6973
                     /locus_tag="ACHJZ2_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4569471.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            6970..7926
                     /locus_tag="ACHJZ2_003422"
     CDS             6970..7926
                     /locus_tag="ACHJZ2_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4569472.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            8013..9062
                     /gene="yahK"
                     /locus_tag="ACHJZ2_003423"
     CDS             8013..9062
                     /gene="yahK"
                     /locus_tag="ACHJZ2_003423"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO4569473.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            9305..10120
                     /gene="yahL"
                     /locus_tag="ACHJZ2_003424"
     CDS             9305..10120
                     /gene="yahL"
                     /locus_tag="ACHJZ2_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO4569474.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEGISNRVQELGDRYYPVNKHVKIRYTL"
     gene            10571..10780
                     /locus_tag="ACHJZ2_003425"
     CDS             10571..10780
                     /locus_tag="ACHJZ2_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569475.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(10797..11468)
                     /gene="yahN"
                     /locus_tag="ACHJZ2_003426"
     CDS             complement(10797..11468)
                     /gene="yahN"
                     /locus_tag="ACHJZ2_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO4569476.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            11615..11890
                     /gene="yahO"
                     /locus_tag="ACHJZ2_003427"
     CDS             11615..11890
                     /gene="yahO"
                     /locus_tag="ACHJZ2_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO4569477.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(11988..13574)
                     /gene="prpR"
                     /locus_tag="ACHJZ2_003428"
     CDS             complement(11988..13574)
                     /gene="prpR"
                     /locus_tag="ACHJZ2_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO4569478.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            13813..14703
                     /gene="prpB"
                     /locus_tag="ACHJZ2_003429"
     CDS             13813..14703
                     /gene="prpB"
                     /locus_tag="ACHJZ2_003429"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO4569479.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            14859..16028
                     /gene="prpC"
                     /locus_tag="ACHJZ2_003430"
     CDS             14859..16028
                     /gene="prpC"
                     /locus_tag="ACHJZ2_003430"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO4569480.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            16062..17513
                     /gene="prpD"
                     /locus_tag="ACHJZ2_003431"
     CDS             16062..17513
                     /gene="prpD"
                     /locus_tag="ACHJZ2_003431"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO4569481.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            17553..19439
                     /gene="prpE"
                     /locus_tag="ACHJZ2_003432"
                     /pseudo
     CDS             17553..19439
                     /gene="prpE"
                     /locus_tag="ACHJZ2_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
BASE COUNT         4733 a         4766 c         5285 g         4717 t
ORIGIN      
        1 aatggcacga gaattgtagg cctgataagc gtagcgcatc aggcatttgt caccattgcc
       61 ggatgcggcg tgaacgcctt atccggccta cgaatggcac gagaatttgt aggcctgata
      121 agcgtagcgc atcaggcagt ctggcgttgg tcataacccc atcaccctct gtagcggaca
      181 taacaacatg aaagaacttg tggtcgttgc cattggtggc aacagcatta tcaaagataa
      241 cgccagccag tcgattgagc atcaggcgga ggcggtgaaa gccgtggccg atacggtgct
      301 ggaaatgctg gcttccgatt acgacattgt gctgacccac ggtaacgggc cgcaggtcgg
      361 gctggattta cgccgtgcgg agattgccca cgagcgcgaa gggctgccct taacgccgct
      421 ggcgaactgt gtggcagata cgcagggcgg cattggctac ctgatccaac aggcgctgaa
      481 caaccggctg gcgcgtcacg gcgagaaaaa agccgtcacc gtggtgactc aggtggaagt
      541 ggataaaaac gatccaggtt ttgcccatcc caccaagccc attggtgcat tcttcagtga
      601 aagccagcgt gacgaattac aaaaggcaaa ccctgactgg cgttttgttg aagatgctgg
      661 acggggctat cgccgcgtgg tcgcctcgcc ggaaccgaaa cgtattgtcg aagcacccgc
      721 cattaaggcg ctgatccaac aaggctttgt ggtgattggc gcgggcggcg gcggaattcc
      781 tgtggtgcgt actgacgcgg gagattacca aagcgtggac gcggttatcg acaaagatct
      841 ctctaccgcg ctgctggccc gtgaaattca cgccgacatt ctggtgatca ccaccggcgt
      901 cgaaaaagtc tgtatccact ttggtaaacc cgagaagcag gcgctggatc gggtggatat
      961 tgccaccatg actcgctata tgcaggaagg acatttcccg cccggcagca tgttgccaaa
     1021 aatcatcgcc agcctgacgt tcctggaaca gggcggtaaa gaagtgatta tcaccacgcc
     1081 ggaatgcctg ccagcggcgc tgcgcggtga aacgggcacc cacattatta aaacgtaagg
     1141 acgtaagatg aaagaaaata atagccgccg tgaatttctg agccagagcg gtaagatggt
     1201 caccgccgcc gcgctgtttg gtacctctgt gccgctcgcc catgcggcgg tctctggcac
     1261 cacaaactgc gaagcgaaca acaccatgaa aatcactgac ccgcattact atctcgacaa
     1321 cgtgctgctg gaaaccggtt ttgactacga aaatggcgtg gcggtgcaga cccgcacggc
     1381 gcgccagacc gtggagattc agaacggcaa aattgtcgcg ctgcgcgaga ataagcagca
     1441 cccggatgcc acgctgccgc actatgacgc tggcgataag ctgatgctgc ccaccacccg
     1501 cgacatgcat attcatctcg acaaaacgtt ttacggcggg ccgtggcgct cgctcaatcg
     1561 cccggcaggc accaccatcc aggacatgat caaactcgag cagaaaatgc tgccggagct
     1621 gcaaccgtac actcaggagc gggcagaaaa actgattgat ttattgcagt cgaaaggcac
     1681 caccattgcc cgcagccact gcaatatcga accggtttcc ggcctgaaaa atctgcaaaa
     1741 tttgcaggcg gtgctggcgc gacgtcaggc gggctttgag tgtgaaatcg tcgccttccc
     1801 gcagcacggt ttgctgctgt cgaaatcgga acccttaatg cgtgaagcaa tgcaggcggg
     1861 tgcgcattac gtcggcgggc tggacccgac cagtgtcgat ggcgcgatgg aaaaatccct
     1921 cgacaccatg ttccagattg cgctggacta cgacaaaggc gtcgatattc acctgcacga
     1981 aaccactccg tcgggcgtgg cagccatcaa ttatatggtt gaaacggtag agaaaacgcc
     2041 tcaactgaaa ggtaagctga ccatcagcca cgcctttgcg ctggctacgc tcaacgagca
     2101 acaggtagat gaactggcgc atcgcatggc ggcgcagcaa atttctatcg cctcgacggt
     2161 gccgattggc acgctgcata tgccgctcaa acagttgcac gacaaaggcg taaaagtgat
     2221 gactggcact gacagcgtta tcgaccactg gtcgccttat ggtctgggcg acatgctgga
     2281 aaaagccaat ctctacgcgc agctctatat tcgtcctaac gaacagaacc tctcccgttc
     2341 gctgtttcta gccactggcg atgtattgcc gctgaatgaa aaaggcgagc gtgtatggcc
     2401 aaaagcgcag gatgacgcca gctttgtgct ggtggacgcc tcctgttccg ccgaggcggt
     2461 ggcgcgtatc tcgccgagaa ccgcaacgtt ccataaaggg caactggtgt gggggagtgt
     2521 ggcaggttga tgcgcatttg aaacgcctgc aacggtgaat agtaagagat ttaagcccca
     2581 gggagtgggg cgggaatggt ttgctaatga atgatcctga actttataat ctcccggcat
     2641 aatttccagg ggattatttg agagaaatac acaaaagtga gttttaacaa aggtatttat
     2701 caggccataa atgtgacctg cttcggataa aaaagattca gtatcgaaaa accaatgata
     2761 ctcttgctga tggttaccaa cagtaattaa gatgtgattt gtttttccgt aatcgcaaaa
     2821 atattgccat gaccataaaa gaaaaattct cacaaaaata tccccatgct tccttttgta
     2881 cttttggcga ttcagccgcg ttggccgatc acctcgcaac gttgattgcg actggtgtta
     2941 aaacagcttc ctgtggttcg ctggcgggat gtattgaaga caacgcattt ccgatgattg
     3001 gtgagtataa gattgtagaa aatagccgtg gtgagcctgt atgtgttatc agagtgattg
     3061 gcctgcattt actgcgcttt tctgatgtca ccgcggagct tgcccgtaag gaaggtgaag
     3121 gtgacctcag tcttgaatat tggcgtaatg aacaccgtcg ttttttccag gcagaaggaa
     3181 gctattcacc agaaatggac gttatctttg aagaatatgc ccttattgac gttgtgtaga
     3241 taactattta tctacatgaa ataagctcac aaacataaaa tatcgctaat tttgtttatt
     3301 ttgctgtttt ttacgtgatg aatgtcacac ttattattgc tgcccgtttt gtccatattt
     3361 ataaccatat ttttaatagc tgcctgaaat agagctgttt attctgcata aaaatattcg
     3421 aaaattctta ttctctccag cgcgctaatt tgggcgaaca cttcctgact accctgcaat
     3481 gaggctgaaa tgatgaataa acgttttgtt atcaatatgg tgtcttcgct gttattaggc
     3541 gctgcgctaa tttctgcgcc gctccaggca gcggaaaaag tcgtggtgaa tatttctaaa
     3601 gtcgatggta tgccgtggtt taatcgcatg ggcgaaggtg tggttgaggc gggtaaagcc
     3661 tttggtgtta atgcctcaca ggtggggcct tcaagtactg atgctccgca acaagttaaa
     3721 attattgagg atttaatcgc ccgtaaggtt aatgcaatta ctatcgtgcc aaacgatgcc
     3781 aacgtgctgg aaccggtatt caaaaaagcg cgagatgccg gaattgtggt tctgaccaac
     3841 gaatcccccg gtcagccaag tgctaactgg gatatcgaaa tcattgataa cgaaaaattt
     3901 gccgctgaat atgttgagca tatggcgaaa cgcatgggtg gcaagggcgg ctatgtgatt
     3961 tacgtgggta gtttgaccgt gccgcagcat aacttatggg ctgacttgct ggtgaaatac
     4021 cagaaagagc attacccgga tatgcatgaa gttacccgcc gcatgccggt tgccgagagt
     4081 gtggatgtct cacgtcgtac tacactggac ctgatgaaaa cctatcctga cttgaaagcg
     4141 gttgtctcct ttggttcaaa cggtccgatt ggcgcgggac gtgcggtgaa agagaaacgg
     4201 gcgaaaaata aagtcgcagt ctatggcatg atgatccctt cacaggcggc atcactgatt
     4261 aaaagtggtg atatcaccga agggattact tacgatccgg cctctgcagg ttacgcactc
     4321 gcagccgtcg ccagcacgtt gctgaagggt gaagagatca agcctggatt agaaatgcag
     4381 aatctgggta aggccgacgt agatatggat aagcgaatta tccgtttcca taaggtatta
     4441 ctggtcaaca aagacaatat cgactcactt tattaatcca tttcttcggg gcgaaagccc
     4501 cgattcttat tgccagggga tgttatggaa accttccttt cccttcgtca tatcaacaaa
     4561 acgtttcatg ccacgcgagc gctgcgcgat gtttctctcg actttatgtc gggtgaagtg
     4621 cactgccttg ccgggcaaaa cggctgtggt aaatcgacgt tgattaaaat tatgtccggt
     4681 gtttatcgcc cggatgaggg agcggaaatt acgcttggtg ggaaaaactg gtcaaagctg
     4741 acacccgccg cttcggtggc gcaggggatt caggtgattt atcaggacct ctctttattt
     4801 cctaacctga gtgtctggga gaatatcgcc gtgaatcact atcaccacgg tctgtttgtt
     4861 aaccgccgtc gtctgcgtga ggtggcgcag gcggcaatga ctagtattaa cgtcacattg
     4921 ccactggata cgctcgtttc tgagctttca attgcccgct gtcagctggt ggcaatttgc
     4981 cgtgcactgg cgcaggacgc acggttgatc gtgatggatg aaccaaccgc ttcgctgacc
     5041 catcaggaag tgcaagggtt attacaggtg gtgcatcaat tgcgtgaacg cgggatctgc
     5101 gtggtctttg tcagtcatcg tctggaagaa gtgatggaag tttccgaccg tatttcagtg
     5161 ctgaaagatg gtgagctggt cgggactttt ccggctgcag agatgaccac aaaacaactc
     5221 ggtttcctga tgaccggcca ggagtttgaa tatcaggtgc gagagttgtg gcagggaaaa
     5281 tccagccccc cggtgctgga ggtgcgtaat ttaagtcgtc atggggaata tctaaatatt
     5341 aacttgcggg tggaagcggg cgaagtggta tcgattgttg gcctgcttgg tgcggggcgc
     5401 acggaattat gtctgagtct gtttggtatg acccgaccag atgctggcga gatcctcatc
     5461 aacggccagc tggttacact gcatagtaac caggacgcca tccgccacgg tattggttat
     5521 gtttcggaag atcgcatgtc gcgcggtttg gtgatggcgc aatctattga agacaacatc
     5581 attagcactg tttttcacaa ggtaaaagat cgcttcggct ttctgagtga aactaaagtg
     5641 cgtgacctgg tggacaggct aatcaaggca ttgaccatca aagtctctga tcctcatctt
     5701 ccggtgaata cgctttctgg tggtaacgcg cagcgagtat cgatcgccaa atggctggcg
     5761 attgggccac gactgttgat tctcgatagc ccgacagtcg gtgttgatat cgccaacaaa
     5821 gcaggaatat acggcattat cagcgatctg gcggctcatg gcattgcggt attgatgatc
     5881 tgcgatgaaa ttgaagaagc gtggtatcaa agccaccgga ttttggtaat gcaaaaaggt
     5941 cagatcaccc atagcttcct gcctgacagc agttctcagg ccaggattgc ggaggtggta
     6001 aatggctgaa ctgaaaaaac gccatgaatt ctggctggca ttactgattg tggtgctgtt
     6061 tgttggcctt gcctggcgca gcgacgagtt tctgacattc ggtaatttgt acgatctcgc
     6121 caataactat gccatgttga ctattctcgc ctgtggtttg tttgtggtgt tgatttccgg
     6181 tggaattgat atttcgtttc cagcaatgac catcattgcg caatacggca tggtgctgtt
     6241 gctgcaaaaa attggtggca acttcgctgt cgcgtttgca ctggcaggct gcatcggcat
     6301 tttacttggc ttaattaacg ccttactggt taatcgccta cgggtgcctt ctatcatcat
     6361 cactatctcg acgctgaata ttttctatgg cctgctgtta tggttgagta aaggtgtgtg
     6421 gctgtacgac tttccgccgt ggtttgagca gggggttatg ttgttcaagt acaccgatgc
     6481 tgatggctat gactatggcc ttggtctgcc gctgatcgcc atgattacgg tggtgctgct
     6541 gacagcgttt atcatgaatt tcaccagtgt agggcgcaaa atttatgccc ttggcgggaa
     6601 ccgcgaatca gccagtcgca tcggctttag cgtgctgaaa ctgcaacttt tcgtctacgg
     6661 ctatatggga ttgatgtctg gcgctgcggg tgtagtgcag tcgtggacgg tgatgactgt
     6721 cgcccccgat tctcttctgg gttatgagct gacagtactg gctgcggtgg tgcttggcgg
     6781 cactagtttg ctcggcgggc gcggcacgtt aactggtact ttgctcggcg tggtgttgtt
     6841 ggcagtgatg caaaacgggc taaatttatt gggagtctcg tcttactggc aaacattgat
     6901 caccggcatc atcatcgttg ccagcattag cgccacggcg tggagtcagc atcagaaccg
     6961 gagcctgcta tgaaaaaatc gtggcgtaat aacgttgagt tttacttgat cggcctgctg
     7021 gtgctaactg ttgccgcgtt cagcattact atgccggaga ttttctggtc gataagtaat
     7081 ttccagtccg tcgcttcgca aatgcccgtg ctgggcattc tggcgttggc tatggcggtg
     7141 accatgcttt gcggtggcat taacttgtca attatagcca ctgctaacgc ctgttcactg
     7201 gtgatggcgt gggtggcgac tcagtatcca ccgggtattg ctacggttgt cgctacgtta
     7261 ctggcgggcg ctggcgcggc ggtgataatc ggtttatgta acggcgtatt gattgctggt
     7321 attcgagtgt tgccaattct tgcaacgctt gggatgatga cgttgctcaa agggataaat
     7381 attctggtga caggtggtag cgccattgcc aattatccct catgggtgct gtggttgaat
     7441 catgcgcaat ggttcggtat tccgctaccg atgtggttgt ttgccgcagt ggcactgggg
     7501 ctgtggatat tgctggagaa aacgccgctc ggtaaagcca tttacctgat tgggtctaac
     7561 gaacgagcaa cactttactc cggcattaac actcgtcggg tgctgatttg ggtttatgtc
     7621 atttccgcct tgctttgtgc ggtagcggca tttttgatga tgtcgaaact caactcggct
     7681 aaggcctctt atggcgaatc gtacttgctg gtgtcaatcc tcgcggcagt actcggcggg
     7741 gttaatccgg acggcggcag cgggcggata attggtatgg tgctggcgct gtttcttctg
     7801 caaattattg aaagcggctt caatattctc ggtataagcc cctacctgac gatggcctta
     7861 tggggaacgt tgctgctctg tttcattcag gctcgcggca tgttggggct ggatcgggtg
     7921 gtttaaatat tggctatatt caatggacgc gttttgccgc gatgacatat caggcgtcgc
     7981 caaatacaca tagctaatca ggagtaaaca caatgaagat caaagctgtt ggttcatatt
     8041 ccgctaaaca accacttgaa ccgatggata tcacccggcg tgaaccggga ccgaatgatg
     8101 tcaaaatcga aatcgcttac tgtggcgtct gccattccga tctccaccag gtccgttccg
     8161 agtgggcggg gacggtttac ccctgcgtgc cgggtcatga aattgtgggg cgtgtggtag
     8221 ccgttggtga tcaggtagaa aaacatgcgc cgggcgattt ggtcggtgtc ggctgcattg
     8281 tcgacagttg taaacattgc gaagagtgtg aagacgggct ggaaaactac tgtgatcaca
     8341 tgaccggcac ctataactcg ccgacgccgg acgaaccggg ccatactctg ggcggctact
     8401 cacaacagat cgtcgttcat gagcgatatg ttctgcgtat tcgtcacccg caagagcagc
     8461 tggcggcggt ggctcctttg ttgtgtgcag ggatcaccac gtattcgccg ctacgtcact
     8521 ggcaggccgg gccgggtaaa aaagtgggcg tggtcggcat cggcggtctg ggacatatgg
     8581 ggattaagct ggcccacgcg atgggggcac atgtggtggc atttaccact tctgaggcaa
     8641 aacgcgaagc ggcaaaagcc ctgggggccg atgaagttgt taactcacgc aatgccgatg
     8701 agatggcggc tcatctgaag agtttcgatt tcattttgaa tacagtagct gcgccacata
     8761 atctcgacga ttttaccacc ttgctgaagc gtgatggcac catgacgctg gttggtgcgc
     8821 ctgcgacacc gcataaatcg ccggaggttt tcaacctgat catgaaacgc cgtgcgatag
     8881 ccggttctat gattggcggc attccagaaa ctcaggagat gctcgatttt tgcgccgaac
     8941 atggcatcgt ggctgatata gagatgattc gggccgatca aattaatgaa gcctatgagc
     9001 gaatgctgcg tggtgatgtg aaatatcgtt ttgttatcga taatcgcaca ctaacagact
     9061 gaaaaaatta ataaatacgc tgtggtttaa catattaact tcgctctcca cttaactttt
     9121 tagttaagga gagcgaaata atatcaaagt agcagtaaaa cctataacgt aaattcaaat
     9181 tgttaaatta actccctcca gtacctaata cttcacacgt tagttatgag cgatttctga
     9241 tagtgcctgg tttaatcaga gctttattat ctgcgacgtt tatttttatt taagagagat
     9301 cgcgatgata tcattaaaag ctccgcacaa taatttgatg ccatatacgc aacaaagcat
     9361 acttaatacg gttaaaaaca atcagttacc agaggatatc aaaagctccc tggtttcctg
     9421 tgtcgatata ttcaaggttt tgattaaaca atattatgat tatccttatg attgtcgtga
     9481 tgatttagtc gatgacgaca aactcattca tctcatggct gccgtacgag actgtgagtg
     9541 gtcagacgat aacgcactca ccataaatgt gcagtttaat gatttccctg gattttatga
     9601 ctggatggat taccctgatc acccggttaa gtttgttttt cgcatacttg aaaatcaaaa
     9661 agggacagtt tgggtatatg accaggatga tgcgtttctt gatattaaag caaatgtgca
     9721 ggctggacgc tttaccgggc ttaagaaatt agtgcagttt atcaattcag tgcgaacaga
     9781 ttgcaaatgt attcttcttg aacaccatat gcctttgctg agacttttcc ctaaagggaa
     9841 agagtgtatg catgtggaaa aatggcttcg cgaaatgtcg tctattccgg agacagacgc
     9901 gcccattaag caggcgcttg cgcatggatt attattacac ttaaaaaata tctaccctgt
     9961 ttttcctgaa tcactggtga tgttattgct ctctgttctg gacgtaaaaa catacagaga
    10021 tgatgcacga ttaaatgaag ggatatctaa tagagtccag gaattaggtg acagatatta
    10081 tcctgtaaat aagcacgtta aaatccgcta cactctttag ttcagaaagg gttccacctg
    10141 caagttgtta ttctacctga gagtgaatcg caatgcaggt aatcatttca tctgtatatc
    10201 tgtatgccaa ctggcattcc atctcttttt tcgccgatat tttgttgtga tcggcgacac
    10261 ttcgcttaaa aaagcaccag tagtggtttc gcagccatgc ggtgtataaa aaaaatgatc
    10321 tcatgcagat gttttgtgaa tgtgtgggtt gacattcata tgaaaaaaat cataattcca
    10381 tcatgtttgt gtggggtctt ttctgtatct tacgcatcgc actcaagcct gacagaaaat
    10441 atgctgtaag gctcatatca aaaaccgcca ttagctcatc aggaagagca gacgacaacc
    10501 aggattgttg tatggtacgg ggttcgaggc cccgatggcg gtccaacttt tcaaaactct
    10561 tctgaattag atccccttat tatcatccct gcaaagtgga acattgccat tgtttggcta
    10621 ttcaggacgg gtcagaccaa tgaaaaaagc ccacacaggg gagagtgggc tgaaatggga
    10681 agctaaagac tcaagtaaac ttatcggaaa taaggaccac gcattacggg ggctatcatc
    10741 gccgatggtc gggattcggc aaatcaggtt gattacgtag agagtaaatt atctgctcac
    10801 cgctgcgtca ccccttcgta aatcaggcgt agcgcgaata caccaataat tgcaccaata
    10861 acccgactgg caacgcgttg catacgccca taagcacgac gcacagcggg caaagaaaac
    10921 gcctgactaa gaaaaactcg ccagataatt gatgcgagca caatccccgc ccaggccatt
    10981 aaacgtgccc atgttggtgt ttcggcattt aatgttactg agaaaatact aataaaaaat
    11041 aaaacggttt gtgggttaga gagatcggta attaatccac ggcgaaaaaa gacataccag
    11101 ggggcgttaa tcggttgttg tagtgtgctc atttgcggtg ttgactggcg gcgcatgctg
    11161 caccacgcaa accataagag ataagcgccg ccgacgattc tgataagcga aaaaatctcc
    11221 tcacactgcg taattagcgt tgcaagacca aacaaaccca acccggaata aaatgcatcg
    11281 cccagcgcca cgcccagtcc ggtcagcact cctgcgcgtc gaccggaagc caggctggtt
    11341 tgtactacca caaaaagatt ggctcccgga ttaaaaaaag taatcacgaa cagtcctacg
    11401 gtcaggtaaa cggcatgcaa aggatccata gtgatttcat ccataaataa gtgaactaac
    11461 tgcatcatat ttctaccaaa aataatcggg tgcgagagag atcacaaagt gtcttatttc
    11521 cggttactgg cgtttatgcc ctgactgaac taattattaa tcaacccaat aatgtcggtg
    11581 ggtgatagtg tgataacaac tctggagccg taatatgaaa ataatctcta aaatgttagt
    11641 cggtgcgtta gcgtttgccg ttaccaatgt ctatgccgct gaattgatga ccaaagcgga
    11701 atttgaaaaa gttgaatcgc agtatgaaaa aataggtgat atttcaacca gtaatgaaat
    11761 gtcgactgca gatgcaaaag aagatttaat caaaaaagcc gatgaaaaag gggctgatgt
    11821 gttggtactg acctccggtc aaactgacaa taagatccac ggcacggcgg atatttataa
    11881 gaagaagtaa ttctgaatcc tatgtaaaca tccccgatgc gtaagtttat cggtgatcat
    11941 ctattgaaat ttatgccgga taaagcgttc gcgctgcatt cggcagttca gcttttcagc
    12001 cgccgccaga acgtcgtccg gctgatgcct aaataattcg ccgctgctgt tttatcgcca
    12061 ttaaatttct ccagtgcctg ttgtggtgtc agcaagcgtg gagcgggaat tttcgccgac
    12121 tcgcgcgcca gttccggcag tagcagctgc aaaaactgcg gcgttaaatc cggcgtcggt
    12181 tccacactta aaaatagcgc cagtcgctcc atcatattgc gcagttcacg aatattacct
    12241 ggccagtcgt agtgcaccag cacggtttcg cttgcctgta atccctggcg taatgcggca
    12301 gaaaatgggg cggagagcgc cgccagagac actttcaaaa agctttccgc cagcggaaga
    12361 atatctgcca cccgctcgcg cagtggtggc aattgcagac gcaaaatact cagccgataa
    12421 aacagatcac ggcgaaactg cccttgccgc atatcttctt ccagattgca gtgagtggcg
    12481 ctaatgaccc gtacatctac cggaacaggc tgatgcccgc cgacgcgggt gacctctttt
    12541 tcttccagca cccgtaacag gcgagtctgc aaaggtagcg gcatttcgcc aatctcatcc
    12601 agaaacagcg tgccaccgtg ggcaatttcg aacagcccgg cgcgacctcc gcgtcgcgag
    12661 ccggtaaacg ccccttcctc atagccaaac agctccgctt ccagcagcga ttcggcaatc
    12721 gccccgcagt tgacggcaac aaacggatgc gactttttgc cctgtcgcgc atcgtggcgg
    12781 gcaaaatatt cccgatgaat tgcctgggcc gccagctctt tgcccgtccc cgtttccccc
    12841 tcaatcaaca ccgccgcact ggagcgggca tacagcaaaa tagtctgccg cacctgctcc
    12901 atctgtggtg attgaccgag catatcgccc agcacgtaac gagtacgcag ggcgttgcgg
    12961 gtggcatcgt gagtgttatg gcgtaacgac atgcgcgtca tatccagcgc atcgctgaac
    13021 gcctggcgca cggtggcggc ggaatagata aaaattccgg tcattccggc ttcttctgcc
    13081 agatcggtaa tcagccccgc gccgaccacc gcttcggtgc cgttagcttt tagctcgtta
    13141 atctgcccgc gtgcgtcttc ttcggtaatg tagctacgtt gatcgaggcg caaattaaag
    13201 gttttttgaa acgccaccag tgccggaata gtttcctgat aagtgacaac gccgatagaa
    13261 gaggtgagtt ttccggcttt tgccagtgcc tgtaacacat cgtagccgct cggtttaatc
    13321 aaaataactg gcactgacag gcggcttttc aggtacgcgc cgttagagcc agcggcgatg
    13381 atggcgtcac agcgttcgtt tgccagtttc ttgcggatgt aggtcactgc tttttcaaag
    13441 ccaagctgaa taggggtaat gttcgccagg tgatcaaact cgaggctgat atcgcgaaac
    13501 agctcgaata agcgcgttac agataccgtc cagataaccg gtttgtcgtc attcagccgt
    13561 ggtggatgtg ccatagcgca ccgcaaagtt aagataccga atattgggtt tagtcttgtt
    13621 tcataattgt tgcaatgaaa cgcggtgaaa cattgcctga aacgttaact gaaacgcata
    13681 tttgcggatt agttcatgac tttatctcta acaaattgaa attaaagatt taattttatt
    13741 aaggcaattg tggcacaccc cttgctttgt cgttatcaac gaaaataaca agttgataac
    13801 aaaggatggg ctatgtctct acactctcca ggtaaagcgt ttcgcgctgc actgactaaa
    13861 gaaaatccat tgcagattgt tggcaccatt aacgccaacc atgcgctgtt ggcgcagcgt
    13921 gccggatatc aggcaattta tctctccggc ggtggcgtgg cggcagggtc gctggggctg
    13981 cccgatctcg gtatttctac tcttgatgac gtgttgacag atattcgccg tatcaccgac
    14041 gtttgttcgc tgccgctgct ggtggatgcg gatatcggtt ttggttcttc tgcttttaac
    14101 gttgcgcgta cggtgaaatc gatgattaaa gccggtgcgg cgggattgca tattgaagat
    14161 caggttggtg caaaacgctg tggtcatcgc ccgaacaaag cgatcgtctc gaaagaggag
    14221 atggtggatc ggatccgcgc ggcggtggat gcgaaaaccg atcctgattt tgtgatcatg
    14281 gcgcgcaccg atgcgctggc ggtagagggg ctggatgcgg cgatcgagcg tgcgcaggcc
    14341 tatgttgaag cgggtgccga gatgttgttc ccggaggcga ttaccgaact cgccatgtac
    14401 cgccagtttg ccgatgcggt gcaggtgcca atcctcgcca acattaccga atttggcgcc
    14461 acgccgctgt ttaccaccga cgaattacgc agcgctcatg tcgcgatggc gctgtacccg
    14521 ctttcagcgt ttcgcgccat gaaccgcgcc gccgaacatg tctacaacgt cctgcgccag
    14581 gaaggcacgc agaaaagcgt catcgacacc atgcagaccc gcaacgagct gtacgaaagc
    14641 atcaactact accactacga agagaagctc gacgacctgt ttgtccgtag ccaggcgaaa
    14701 taaaaaacgc ccgttggttg tattcgacaa ccgatgcctg atgcgccgct gacgcgactt
    14761 atcaggccta cggggtgaac tgtaggtcgg ataagacgca tggcgtcgca tccgacaaca
    14821 atctcgaccc tacaaatgat aacaatgacg aggacaatat gagcgacaca acgatcctgc
    14881 aaaacagtac ccatgtcatt aaaccgaaaa aatctgtggc gctttctggc gttccggcgg
    14941 gcaatacggc gctctgcacc gtgggtaaaa gtggcaatga cttgcattac cgtggctacg
    15001 atattcttga tctggcggaa cattgcgaat ttgaagaagt ggcgcatctg ctgatccacg
    15061 gcaaactgcc gacccgtgac gaactcgccg cctacaaaac gaaactgaaa gccctgcgcg
    15121 gtttaccggc taacgtgcgt accgtgctgg aagccttacc ggcggcgtca cacccgatgg
    15181 atgttatgcg caccggcgtt tccgcgctcg gctgcacgct gccagaaaaa gaggggcata
    15241 ccgtctctgg cgcgcgggat attgccgaca aactgctggc gtcacttagc tcgattcttc
    15301 tctactggta tcactacagc cacaacggcg aacgcatcca gccggaaacc gatgacgact
    15361 ccatcggtgg tcacttcctg catctgctgc acggcgaaaa gccgtcgcaa agctgggaaa
    15421 aggcgatgca tatttcgctg gtgctgtacg ccgaacacga gtttaacgcc tccaccttta
    15481 ccagccgggt gattgcgggc actggctctg atatgtattc cgcgattatt ggcgcgattg
    15541 gtgcactgcg cgggccgaag cacggcgggg cgaatgaagt gtcgctggag atccagcaac
    15601 gctacgaaac gccggacgaa gccgaagccg atatccgcaa gcgggtggaa agcaaagaag
    15661 tggtcattgg ttttggtcat ccggtttata ccatcgccga cccgcgccac caggtgatta
    15721 aacgtgtggc gaagcagctc tcgcaggaag gcggctcgct gaagatgtac aacatcgccg
    15781 atcgcctgga aacggtgatg tgggagagca aaaagatgtt ccccaatctc gactggttct
    15841 ccgctgtttc ctacaacatg atgggcgttc ccaccgagat gttcacacca ctgtttgtta
    15901 tcgcccgcgt caccggctgg gcggcgcaca ttatcgaaca acgtcaggac aacaaaatta
    15961 tccgtccttc cgccaattat gttggaccgg aagaccgcca gtttgtcgcg ctggataagc
    16021 gccagtaaac ctctacgaat aacaataagg aaacgtaccc aatgtcagct caaatcaaca
    16081 acatccgccc ggaatttgat cgtgaaatcg ttgatatcgt cgattacgtc atgaactacg
    16141 aaatcagctc caaagtagcc tacgacaccg cacattactg tctgctcgac acgctcggct
    16201 gcggtcttga agctctcgaa tacccagcct gtaaaaaact gctggggcca attgtccccg
    16261 gcaccgtcgt acctaacggc gtgcgcgtcc ccggaactca gttccagctc gaccccgtcc
    16321 aggcggcatt taacatcggc gcgatgatcc gctggctcga tttcaacgat acctggcttg
    16381 cggcagagtg gggccatcct tccgacaatc tcggcggcat tctggcaacg gcggactggc
    16441 tttcacgcaa cgcggtcgcc agcggcaaag cgccgttgac catgaaacag gtgctgaccg
    16501 gaatgattaa agcccatgaa attcagggct gcatcgcgct ggaaaactcc tttaaccgcg
    16561 tcggcctcga ccacgttctg ttagtgaaag tggcttccac cgccgtggtc gccgaaatgc
    16621 ttggcctgac ccgcgaggaa attctcaacg ctgtttcgct ggcgtgggtg gacggtcagt
    16681 cgctgcgcac ctatcgccat gcgccgaaca ccggcacgcg taaatcctgg gcggcgggcg
    16741 atgccacttc ccgcgcggta cgtctggcac tgatggcgaa aacgggcgaa atgggttacc
    16801 cgtcagccct gactgcgcct gtgtggggct tctacgacgt ctcctttaaa ggtgaatcgt
    16861 tccgcttcca gcgtccgtac ggttcttacg tgatggaaaa tgtgctgttc aaaatctcct
    16921 tcccggcgga gttccactcc cagacggcag ttgaagcagc gatgacgctc tatgaacaga
    16981 tgcaggcagc aggcaaaacg gcggcagata tcgaaaaagt gtccatccgc acccacgaag
    17041 cctgtattcg catcatcgac aaaaaggggc cgctcaataa cccggcagac cgcgaccact
    17101 gcattcagta catggtggcg atcccactgc tattcgggcg cttaacggcg gcagattacg
    17161 aggacaacgt tgcgcaagat aaacgcattg acgccctgcg cgagaagatc aattgctttg
    17221 aagatccggt atttaccgct gactaccacg acccggaaaa acgcgccatc gccaatgcca
    17281 ttacccttga gttcaccgac ggcacacgat ttgaagaagt ggtggtggag taccccattg
    17341 gtcatgctcg ccgccgtcag gatggtattc cgaaactggt cgataaattc aaaatcaatc
    17401 tcgcgcgcca gttcccgact cgccaacagc agcgcattct ggaggtttct ctcgacagag
    17461 ctcgcctgga acagatgccg gtcaatgagt atctcgacct gtacgtcatt taagtaaacg
    17521 gcggtaaggc gtaagttcaa caggagagca ttatgtcttt tagcgaattt tatcagcgtt
    17581 cgattaacga accggagcag ttctgggccg agcaggcccg gcgtattgac tggcagacgc
    17641 cctttacgca aacgctcgat cacagcaatc cgccgtttgc ccgttggttt tgtgaaggcc
    17701 gaaccaactt gtgccacaac gctatcgacc gctggctgga gaaacaacca gaggcgctgg
    17761 cgctgattgc cgtctcttcg gaaacagaag aagagcgcac ctttaccttt cgccagcttc
    17821 atgacgaagt aaacgcggtg gcgtcaatgc tgcgctcgct gggcgtgcag cgtggcgatc
    17881 gggtgctggt gtatatgccg atgattgccg aagcgcatat tactctgctg gcctgcgcgc
    17941 gcattggcgc tattcactcg gtggtgtttg ggggatttgc ctcgcacagc gtggcggcgc
    18001 gaattgatga tgctaaaccg gtgctgattg tctcggctga tgccggagcg cgcggtggca
    18061 aaatcattcc ctataaaaaa ttgctcgacg atgcgataag tcaggcgcag caccagccac
    18121 gccatgtttt gctggtggat cgcgggctgg cgaaaatggc acgcgtcagc gggcgggatg
    18181 tcgatttcgc gtcgttgcgc catcaacaca tcggcgcgcg ggtaccggtg gcgtggctgg
    18241 aatccaacga aacctcctgc attctctaca cctccggcac gaccggcaaa cctaaaggcg
    18301 tgcagcgtga cgttggcgga tatgcggtgg cgctggcgac ctcgatggac accatttttg
    18361 gcggcaaagc gggcggtgtg ttcttttgtg cttcggatat cggctgggtg gtggggcatt
    18421 cgtatatcgt ctacgcgccg ctgctggcgg ggatggcgac tatcgtttac gaaggattac
    18481 cgacctggcc ggactgcggc gtgtggtgga aaatcgtcga gaaatatcag gttagccgga
    18541 tgttctcagc gccgaccgcc attcgcgtgc tgaaaaaatt ccctaccgct gaaattcgca
    18601 aacacgatct ctagtcgctg gaagtgctct atctggctgg agaaccgctg gacgagccga
    18661 ccgccagttg ggtgagcaat acgctggatg tgccggtcat cgacaactac tggcagaccg
    18721 aatccggctg gccgattatg gcgattgctc gcggtctgga cgacaggccg acgcgtctgg
    18781 gaagccccgg cgtgccgatg tatggctata acgtgcagtt gcttaatgaa gtcaccggcg
    18841 aaccgtgtgg cgtcaacgag aaagggatgc tggtggtgga agggccgctg ccgccggggt
    18901 gtattcagac catctggggc gacgacgacc gctttgtgaa gacgtactgg tcgctgtttt
    18961 cccgcccggt gtacgccacc tttgactggg gcatccgtga cgctgacggt tatcacttta
    19021 ttctcgggcg cactgacgat gtgattaacg ttgccgggca tcggctgggg acgcgcgaga
    19081 ttgaagagag tatctccagt catccgggcg ttgccgaagt ggcggtggtt ggggtgaaag
    19141 atgcgctgaa agggcaggtg gcggtggcgt ttgtcattcc gaaagagagc gacagtctgg
    19201 aagatcgtga tgtggcgcat tcgcaagaga aggcgattat ggcgctggtg gacagccaga
    19261 ttggcaactt tggccgcccg gcgcacgtct ggtttgtctc gcaattgcca aaaacgcgat
    19321 ccggaaaaat gctgcgccgc acgatccagg cgatttgcga aggacgcgat cctggggatc
    19381 tgacgaccat tgatgatcct gcgtcgttga atcagatccg ccaggcgatg gaagagtaag
    19441 gtattgtcgg atgcgtcgcg tggcgcatcc gacagttgtg cgcagatgcc tgatgcgacg
    19501 c
//
LOCUS       DAZFBR010000087        19259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFBR010000087 DAZFBR010000000
VERSION     DAZFBR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.087"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(328..1977)
                     /gene="pgi"
                     /locus_tag="ACHJZ2_003433"
     CDS             complement(328..1977)
                     /gene="pgi"
                     /locus_tag="ACHJZ2_003433"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO4569482.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2214..2410
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2502..3851
                     /gene="lysC"
                     /locus_tag="ACHJZ2_003434"
     CDS             2502..3851
                     /gene="lysC"
                     /locus_tag="ACHJZ2_003434"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO4569483.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4104..4376
                     /locus_tag="ACHJZ2_003435"
     CDS             4104..4376
                     /locus_tag="ACHJZ2_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO4569484.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4377..5249)
                     /gene="rluF"
                     /locus_tag="ACHJZ2_003436"
     CDS             complement(4377..5249)
                     /gene="rluF"
                     /locus_tag="ACHJZ2_003436"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO4569485.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5597..6544
                     /locus_tag="ACHJZ2_003437"
     CDS             5597..6544
                     /locus_tag="ACHJZ2_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO4569486.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6614..7420
                     /locus_tag="ACHJZ2_003438"
     CDS             6614..7420
                     /locus_tag="ACHJZ2_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4569487.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7430..7837
                     /locus_tag="ACHJZ2_003439"
     CDS             7430..7837
                     /locus_tag="ACHJZ2_003439"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO4569488.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7837..8331
                     /locus_tag="ACHJZ2_003440"
     CDS             7837..8331
                     /locus_tag="ACHJZ2_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO4569489.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            8397..9194
                     /locus_tag="ACHJZ2_003441"
     CDS             8397..9194
                     /locus_tag="ACHJZ2_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO4569490.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLLAPASTALDQLDD"
     gene            9205..10029
                     /locus_tag="ACHJZ2_003442"
     CDS             9205..10029
                     /locus_tag="ACHJZ2_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO4569491.1"
                     /translation="MEQRKITRGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10081..11310
                     /gene="sorE"
                     /locus_tag="ACHJZ2_003443"
     CDS             10081..11310
                     /gene="sorE"
                     /locus_tag="ACHJZ2_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO4569492.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTNNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11693..12382
                     /gene="pepE"
                     /locus_tag="ACHJZ2_003444"
     CDS             11693..12382
                     /gene="pepE"
                     /locus_tag="ACHJZ2_003444"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO4569493.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12473..14104)
                     /locus_tag="ACHJZ2_003445"
     CDS             complement(12473..14104)
                     /locus_tag="ACHJZ2_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO4569494.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14324..18007)
                     /gene="metH"
                     /locus_tag="ACHJZ2_003446"
     CDS             complement(14324..18007)
                     /gene="metH"
                     /locus_tag="ACHJZ2_003446"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO4569495.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18207..19031
                     /gene="iclR"
                     /locus_tag="ACHJZ2_003447"
     CDS             18207..19031
                     /gene="iclR"
                     /locus_tag="ACHJZ2_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO4569496.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4562 a         5001 c         5045 g         4651 t
ORIGIN      
        1 aaaataaaag taaaagtaat taatctgagt attttccgaa atcaatcacg attaaaaatc
       61 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
      121 gataaaacaa tgccgactta tagtcggcat tgtttttaag gttatttaat taatcgtgct
      181 atgcattaat tgccgaatac gatgtacata tcggcatcga cctgtaggcc tgataagacg
      241 cgtaaagcgt cgcatcaggc accagttgcc ggatgcggcg taaacgcctt atccggccta
      301 catagcggca tcaggcatga acgatgatta accgcgccac gctttatagc ggttaatcag
      361 accattggtc gagctatcgt ggctgctgat ttctttatca tctttcagct ctggcagaat
      421 acggttcgcc agctgtttac ccagttccac gccccactgg tcgaaggtga agatgttcag
      481 gatcacgccc tgagtaaaga ttttgtgctc atacagcgca atcaacgcac ccaggctgaa
      541 cggagtgatt tcgcgcaaca ggatagagtt ggtcgggcgg ttaccttcga acactttgaa
      601 cggcaccacg tagtcaagcg ttgccggatc tttaccctga tcgcgatatt cctgctcaac
      661 cacttcgcgg gatttaccaa acgccagcgc ttcggtctgg gcgaagaagt tagacagcag
      721 tttctggtgg tgatcagaga gcgggttatg ggtgatagcc ggagcgatga aatcgcacgg
      781 taccattttg gttccctggt ggatcagctg gtagaacgcg tgctgaccgt tagtgcctgg
      841 ttcaccccag ataatcgggc cagtctggta atccacaacg ttaccgttac ggtcaacata
      901 cttaccgttg gactccatat tgccctgctg gaagtacgcc gcgaaacggt gcatatactg
      961 gtcatacggc agaatcgctt cagtttccgc accaaagaaa ttgttgtacc agatgccaat
     1021 cagcgccaac agtacaggca ggtttttctc ggcaggcgtg gtggagaaat gcttgtccat
     1081 cgcgtgtgcg ccagaaagca gttcaacgaa gttatcaaag ccgatggaga gaacaatcga
     1141 caggccaatc gctgaccaca aagagtaacg accgccgacc cagtcccaga actcgaacat
     1201 gttggcagta tcaataccaa actcgccaac ggctttggca ttggttgaga gcgccgcaaa
     1261 gtgtttcgca acgtgttttt catcacctgc cgctttcagg aaccagtcac gcgcgctatg
     1321 ggcgttggtc atggtttcct gagtggtgaa ggttttagat gctaccagga acagcgtggt
     1381 ttccgggttt acttttttca gcacttcagc gatgtgagtc ccatcgacgt tagaaacaaa
     1441 gtgcatgttc aggtgatttt tgtacggacg cagtgcttcg gtcaccatgt acgggccgag
     1501 gtcagaaccg ccgatcccga tgttcactac gtcagtgatt gctttgccgg tataaccctt
     1561 ccactcaccg gagataatcg cttccgagaa ggttttcatc ttctccagca ccgcgttgac
     1621 ttctggcata acatctttgc catcaaccag aatcggggta ttgctacgat tacgcagcgc
     1681 tacgtgcagc acggcgcggt tttcagtgcg gttgatcttc tcgccagaga acatcgactt
     1741 aatcgcgccc gccagatcgc actctttcgc cagatcttgt aatttcgcca gcgtctcttc
     1801 agtgatgcgg tttttggagt aatccaccag catctgatcg tcgaaggttg cggagaactt
     1861 agaaaaacga tcgccgtctt tagcaaaaag atcggcgatc gtaacgtctt tcatttcatc
     1921 gaagtgtttc tgtagtgcct gccaggcagc ggtctgcgtt ggattgatgt ttttcattag
     1981 caatactctt ctgattttga gaattgtgac tttggaagat tgtagcgcca gtcacagaaa
     2041 aatgtgatgg ttttagtgcc gttagcgtaa tgttgagtgt aaacccttag cgcagtgaag
     2101 catttattag ctgaactact gaccgccagg agtggatgaa aaatccgcat gaccccgtcg
     2161 ttgacaaccg ccccgctcac cctttattta taaatgtact acctgcgcta gcgcaggcca
     2221 gaagaggcgc gttgcccaag taacggtgtt ggaggagcca gtcctgtgat aacacctgag
     2281 ggggtgcatc gccgaggtga ttgaacggct ggccacgttc atcatcggct acaggggctg
     2341 aatcccctgg gttgtcacca gaagcgttcg cagtcgggcg tttcgcaagt ggtggagcac
     2401 ttctgggtga aaatagtagc gaagtatcgc tctgcgccca cccgtcttcc gctcttccct
     2461 tgtgccaagg ctgaaaatgg atcccctgac acgaggtagt tatgtctgaa attgttgtct
     2521 ccaaatttgg cggtaccagc gtagctgatt ttgacgccat gaaccgcagc gctgatattg
     2581 tgctttctga tgccaacgtg cgtttagttg tcctctcggc ttctgctggt atcactaatc
     2641 tgctggtcgc tttagcggaa ggactggaac ctggcgagcg attcgaaaaa ctcgacgcta
     2701 ttcgcaacat ccagtttgcc attctggaac gtctgcgtta cccgaacgtt atccgtgaag
     2761 agattgaacg tctgctggag aacattactg ttctggcaga agcggcggcg ctggcaacgt
     2821 ctccggcgct gacagatgaa ctggtcagcc acggcgagct gatgtcgacc ctgctgtttg
     2881 tcgagatcct gcgcgaacgc gatgttcagg cacagtggtt tgatgtacgt aaagtgatgc
     2941 gtaccaacga ccgatttggt cgtgcagagc cagatgtagc cgcgctggcg gaactggccg
     3001 cgctgcagct gctcccacgt ctcaatgaag gcttagtgat cacccaggga tttatcggta
     3061 gcgaaaataa aggtcgtaca acgacgcttg gccgtggagg cagcgattat acggcagcct
     3121 tgctggcgga ggctttacac gcatctcgtg ttgatatctg gaccgacgtc ccgggcatct
     3181 acaccaccga tccacgcgta gtttccgcag caaaacgcat tgatgaaatc gcgtttgccg
     3241 aagcggcaga gatggcaact tttggtgcaa aagtactgca tccggcaacg ttgctacccg
     3301 cagtacgcag cgatatccca gtctttgtcg gctccagcaa agacccacgc gcaggtggta
     3361 cgctggtgtg caataaaact gaaaatccgc cgctgttccg cgcgctggcg cttcgtcgca
     3421 atcagactct gctcactttg cacagcctga atatgctgca ttctcgcggt ttcctcgcgg
     3481 aagttttcgg catcctcgcg cggcataata tttcggtaga cttaatcacc acgtcagaag
     3541 tgagcgtggc attaaccctt gataccaccg gttcaacctc cactggcgat acgttgctga
     3601 cgcaatctct gctgatggag ctttccgcac tgtgccgggt ggaggtggaa gaaggtctgg
     3661 cgctggtcgc gttgattggc aatgacctgt caaaagcctg cggcgttggc aaagaggtat
     3721 tcggcgtact ggaaccgttc aacattcgca tgatttgtta cggcgcatcc agccataacc
     3781 tgtgcttcct ggtgcccggc gaagatgccg agcaggtggt gcaaaaactg catagtaatt
     3841 tgtttgagta aatactgtat ggcctggaag ctatatttcg ggccgtattg attttcttgt
     3901 cactatgctc atcaataaac gagcctgtac tctgttaacc agcgtcttta tcggagaata
     3961 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
     4021 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
     4081 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
     4141 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
     4201 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
     4261 agcgtgaggc tgaagcgaaa aaagcccgcc agttaaagaa aaagcaggct tataaaccgg
     4321 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctgattag
     4381 cgccccttct tttttcgccc cggcgaggta aaacgcttac cgttggacgc cgggcgaccg
     4441 cctttttccg ccgttttttc catcttaacg actggacgtt tgatgcccgc tgttttcggt
     4501 ttggccttcg ctttaggttt tgcctcggaa gaggaatttt cgatgagctt aaagaggtcg
     4561 attaactcat cgtcggttaa atcgcgccac tcccccagcg gaatgccgct taagctgacg
     4621 ttcataatgc gcgtacgttc cagctttttc acttcatagc cgaaatgctc gcacatgcgc
     4681 cggatctgac ggttcagccc ctgcaccagg gtaatgcgga agacaaacgg cgcttctttt
     4741 ttgactttgc actttttagt caccgtcccg aggatcggca cccccgcccc cataccacga
     4801 ataaattcat cggtaatcgg tttatcgacc gtcaccagat actctttctc atgatcgtta
     4861 cccgcacgga ggatcttatt caccagatcg ccgtgattgg tgaggaaaat cagcccctgg
     4921 gagtctttat ccaggcggcc aatcgggaac acgcgtttgc tgtggttaac gaaatcgaca
     4981 atgttatcgc gctcgccatc ttcggtggtg ctgacaatgc ctacgggctt gttcagggcg
     5041 ataagtacca aatcttcggc ttcccgaggc tcaatcaact gaccgtttac tttcacaacg
     5101 tcgccgggtt tcacctgatc gccaatggtg gctcgcttgc cattaaggaa cacattgcct
     5161 tgctcgatat agcgatccgc ttcgcggcgt gagcaaattc cgctttcgct gatgtattta
     5221 tttaaacgga ctgatgagtc gggcagcata aattctcctg taaaacgcgg aatataccgt
     5281 acctcttagc ggggaaaaac agggttatgc ggcaacgtcg aaattaaatg tgatctgcca
     5341 cgccttttat cacaaaagtg tctccggaag tacaatgcaa atttgcgtgg caaaataagg
     5401 ataatcaaca tattacgaat cgctgcacac ctgtgctgat aactactgcg cagagaaaca
     5461 aatgtgcaag agaaagcgct atttctcccc atgcaaagag tgaaaaatcg ctttgaaagg
     5521 ccatcatgat ggggttatat cagatcaagc ggtgagaaag cgtatttgca caaatgtgca
     5581 gaaccaggag gcggcaatgg aaaacagtga cgatatccgt ttgattgtga agattgccca
     5641 actctattac gaacaggata tgacgcaggc gcaaatcgcg cgcgaactgg gtatttaccg
     5701 caccaacatc agccgcttgc ttaaacgagg ccgcgatcag ggaattgtca ccatcgccat
     5761 caactatgac tacaacgaaa atctctggct ggagcagcaa ctgaagcaaa agtttggcct
     5821 gaaagacgtt gtggtggtgt cgggaaatga tgaggatgaa gagactcaac tggcgatgat
     5881 ggggttacac ggcgcgcaac tgctggatcg cttgctggag cctggcgata ttgtcggttt
     5941 ttcctggggt cgcgcggtga gcgcactggt tgaaaacttg ccgcaggcgg ggcaatcgcg
     6001 gcagttaatc tgcgtaccaa ttattggcgg accgtccggt aaactcgaaa gccgctatca
     6061 cgtaaacaca ttaacctaca gcgcggcagc gaagctgaaa ggggaatcgc atctcgcgga
     6121 ttttccggct ctactggata acccattaat tcgtaatggg atcatgcagt ctcagcactt
     6181 taaaaccatc tctgcctact gggataatct ggatgtcgcc ctggtgggaa ttggctcacc
     6241 ggccattcgc gacggcgcta actggcatgc gttttatggt ggtgaagaga gtgacgacct
     6301 gaatgcccgc caggttgctg gcgatatttg ctcgcgcttt tttgatattc acggcgaaat
     6361 ggttgaaacg aatatgagcg aaaaaacact ctctatcgaa atgaataaat taaagcaggc
     6421 acgatattcc attggcattg ccatgagtga agaaaaatac agcggaatta ttggtgcact
     6481 gcgtggaaaa tatattaatt gtctggtaac gaatagcagc acagctgaac tattactgaa
     6541 ataaaaatct tcccgatgtt tatcgtaata aaaattacgg cgggtcccta ttatctaaaa
     6601 tcaggagtct gttatgcaaa cgtggttaaa tttgcaggat aaaatcatta tcgtcaccgg
     6661 cggcgcatcc ggtattggtc tggcgattgt agaggaatta ttagcacaag gcgcaaacgt
     6721 acagatggta gatattcacg gtggcgatgg acaatatgaa ggtcataaag gctatcagtt
     6781 ttggccgacc gatatttcca gcgccaaaga ggtaaatcat acggtagcgg aaattatcca
     6841 gcgttttggt cgcatcgacg gtctggtcaa taacgccggg gtcaatttcc cgcgtctgct
     6901 ggtcgatgag aaagcgcctg ccgggcagta tgaactcaac gaagctgcat tcgaaaaaat
     6961 ggtcaatatc aaccagaaag gcgtttttct gatgtcgcag gcggtggcgc gacagatggt
     7021 caaacaacat gatggcgtga ttgtgaatgt ttcctcagaa agtgggctgg aaggctcaga
     7081 aggccaaagc tgttacgccg ctaccaaagc cgcgctcaat agcttcacgc gctcctggtc
     7141 gaaagagctg ggtaaacacg gtatccgtgt ggtcggtatc gcgccgggga ttctggaaaa
     7201 aaccgggcta cgcacgccgg aatatgaaga ggcgctggcg tggacgcgca atatcaccgt
     7261 cgagcagctg cgtgaaggct ataccaaaaa cgccattcct attgggcgcg ccggaagatt
     7321 agcagaaatc gctgattttg tttgttatct gctgtctgaa cgcgccagct atatcaccgg
     7381 agtaaccact aacattgcgg gcggcaaaac gcgcggctaa ggaggcttta tggtcaacgc
     7441 cattttttgt gcccacggca aattagcttg cgccatgctg gaatcggttc agatggttta
     7501 cggcaacgcc aatgttgaag ccgttgaatt tgtgcccggt gaaaacgccg gagacatcgt
     7561 agcaaagctg gaaaaattag taagcattca caatcaaaat gagtggctga ttgccgtgga
     7621 tttacagtgc ggcagcccgt ggaatgcggc agcgatgctt gcgatgcgca atcctcgtct
     7681 gcgggtaatc agcggacttt ctctgccgct ggcgctcgaa ctggttgata accaggacag
     7741 catgaatgtt gatgaactgt gtgaacacct gacgcaaatc gcaaagcaaa cctgcgttgt
     7801 ctggaaacaa ctggcaacga cagaggagga tttctgatga acattacgct cgcccgcatt
     7861 gatgaccgcc tgatccacgg tcaggtcact accgtctggt cgaaagtggc taacgcccag
     7921 cgcattatta tctgcaatga cgaagtttat aacgatgaag ttcgccggac attattgcgt
     7981 caggctgcgc caccgggaat gaaggttaac gtggtcaata ttgaaaaagc cgtcgcggtt
     8041 tatcacaacc cgcaatatca ggacgaaacc gttttttatc ttttcactcg cccacaggat
     8101 gccttagcga tggttcgcca gggggtaaaa ataggcaccc tgaatattgg cggcatggcc
     8161 tggcggcccg gtaaaaaaca gttaaccaaa gcggtttcat tagatgatga tgatattaac
     8221 gcatttcatg agttaaataa tctaggcgtc atactggatt tacgcgtcgt agcgtcagat
     8281 ccctcaatca atattatcta caaaataaac gaacagttaa tcgcaaatta aataatagcg
     8341 cctgtgtaca tatgctttaa caggcaggga tgacgacact taaaaggtga caaatcatgg
     8401 aaataagtac cctacaaatc attgctatat ttcttttttc ctgtattgcc ggaatgggca
     8461 gcgtgctgga tgaatttcag actcatcgcc cgttaattgc ctgtacggtg attggtttaa
     8521 ttctcggtga tttaaaaacc ggaataatac tcggtggtac gctggaattg atagctctcg
     8581 gctggatgaa cgtcggcgcg gcgcaatctc cggattctgc actcgccagc ataatctccg
     8641 ccattctggt tatcgttggt cagcaaagca tcgccaccgg aatcgccatc gcgttgcctg
     8701 tggctgcggc aggccaggtg ctgacggtgt ttgcccgtac catcaccgta gtgttccagc
     8761 acgcggcgga taaagcagcg gaagaagcac ggtttcgcac tctcgatatt ctgcatgtct
     8821 ccgcgcttgg cgtgcaggcg ctgcgcgtcg ccattccggc actgattgtc tcgctgttcg
     8881 tcagcgccga tatggtgagc aatatgttaa acgccattcc cgaatttgtg acccgaggtc
     8941 tgcaaattgc tggcggtttt atcgtggtag tcggttacgc catggtgcta cgtatgatgg
     9001 gcgtgaaata tttgatgcct ttctttttcc tcggtttcct cgcaggtggc tacctcgatc
     9061 tcagtctgct ggcgttcggt ggcgtcggcg tgatcatggc cctggtctac atccagctaa
     9121 atccacagtg gcgtaaagct gaacctcaac tcctggcccc cgcttccacc gcccttgacc
     9181 aacttgacga ttaacggagc catcatggaa cagagaaaaa ttacacgcgg cgatctggtg
     9241 agcatgtttc tgcgctccaa cctgcaacag gcgtccttta actttgaacg tattcacggg
     9301 ctgggttttt gctacgacat gatccccgcc atcaagcgac tttacccagt aaaagaggat
     9361 caggttgcgg cgctcaggcg acacctggtg ttctttaata ccacgccagc cgtatgtggc
     9421 ccggtcatcg gcgtcactgc cgccatggaa gaggcgcggg ccaacggcgc ggaaattgat
     9481 gacggcacca ttaacggcat caaagtcggt ctgatgggac cattggcagg ggttggcgat
     9541 ccactggtct ggggaacgct gcgcccgatt accgccgcgc tcggcgcatc gctggcgctg
     9601 tctggcaaca ttctcggccc gctgctgttc ttctttattt tcaacgcggt gcgtctggcg
     9661 atgaagtggt atggcctaca gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg
     9721 ggcgggaatg tgctgcaaaa actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg
     9781 ggcgtgctgg tcaccaaatg gacgtcaatc aacgtaccgt tggtggtttc acaaacgcat
     9841 gccgccgatg gctccaccgt caccatgacc gtgcagaaca ttctcgatca actttgccct
     9901 ggtttgctgg cgctcggtct gacgctactg atggttcgtc tgctcaacaa aaaaattaac
     9961 ccggtatggc tgattttcgc cctgtttggc ttagggatta tcggcaatgc gctgggcttc
    10021 ctgtcctgat tcttcgcccc ggcacgactg ccggggccat cgttcaacat gaggtggttt
    10081 atgaaaacaa cagctctgcg tctttatggt aaacgtgatt tacgcctgga aacctttgac
    10141 cttcctgaaa tgcaggagga tgaaatcctc gcgacggtgg tcactgacag cctgtgcctc
    10201 tcttcctgga aagaggccaa tctgggtgaa aaccataaaa aagtacccga cgatgtggcg
    10261 accaacccaa tcatcatcgg ccacgagttt tgcggcgata ttctggccgt gggtaaaaag
    10321 tggcagcaca aattccagcc gggtcagcgt tatgtgattc aggccaacct gcaactcccc
    10381 gaccgcccgg actgccccgg ctactccttc ccgtgggtag gcggcgaggc cacgcatgtg
    10441 gttattccca acgaggtcat ggaacaagat tgcctgctgg catacgacgg cgaaacctat
    10501 tttgaaggct cgctggttga accgctttcc tgcgtgattg gcgcgttcaa cgccaactat
    10561 catcttcagg aaggtagtta taaccacacg atggggattc gcccgcaagg gcgcatgctg
    10621 atcctcggcg gcaccggacc aatgggactg ttggcgattg attatgcgct acatggaccc
    10681 gttaacccgt cgctgctcgt cattaccgat accaacaacg ataaattgag ttatgcgcgc
    10741 aagcactatc catcagaacc gcaaacactg attcattatc tcaatgccgc cgatgcagca
    10801 tttgatacgc taatggcgct gagtggcggt cacggcttcg atgatatttt cgtctttgtg
    10861 cctaatgaag gactggtgac tctcgcctct tccttgctgg cgacagatgg ttgcctgaat
    10921 ttcttcgccg gaccgcagga taaacatttc agcgcgccaa ttaatttcta cgatgtgcat
    10981 tatgcattta cccactacgt gggcacgtca ggcggcaata ccgacgacat gcgcgcagcg
    11041 gtcaaattga ttgaagagaa aaaagtgcag gccgcaaaag tggtaacaca tattcttggg
    11101 ctgaatgccg cgggcgaaac cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg
    11161 gtgtataccg ggaaatacct gccgctgacg tcactcacgc agattcagga tcaagcactg
    11221 gcggcgattc tggcgcgtca tcaggggatc tggtccggtg aggcggagca atatctgctc
    11281 actcatgcag aggcaatttc ccatgattaa tcgcgctact taactctgca tgtcgctggc
    11341 tggtcaccgc ggcaacttcg cgacctgatg tgcggctgat tgctgaagcg gcccaatgtg
    11401 cacgagaagg ataaaaaaac atcagcgcct taaggggcgc tcaaattggt ggcaaagtac
    11461 acattgttta tgccggatgc ggaataaagc cttattcggc ctacataatc tcgaaaattc
    11521 aatatattgc agttatctgg taggcctggt aagcgtagcg catcaggcaa tgctgtgttt
    11581 gtcatcagtc tcagcgcccg gtgcaatacg tgacatcgtg agccatgtca gtaagtggtt
    11641 aaattcgcag tggtagcatg ccttccgtga tttcttacta accggagaag taatggaact
    11701 gcttttattg agtaactcga cgctgccggg taaagcctgg ctggaacatg cactgccgct
    11761 gattgctgaa cagttgcagg gtcgccgctc agcggtgttt atccctttcg ctggcgtaac
    11821 gcagacctgg gatgattaca cagcgaaaac ggctgcggtt ctcgctccgc tgggtgtttc
    11881 tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg cggaaattgt
    11941 gattgtcggc ggcggtaata ctttccagtt gctgaaacag tgccgcgagc gcgggctgct
    12001 ggctccaatt actgacgtgg ttaaacgtgg cgcgctgtat attggctgga gcgcaggcgc
    12061 taaccttgct tgcccaacca ttcgtaccac caacgatatg ccgattgtcg atccgcaagg
    12121 tttcgatgcg ctaaatctgt tcccgttgca aatcaacccg cacttcacca acgcgctgcc
    12181 ggaaggccat aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc
    12241 agaactgacg attattggtc taccggaagg taactggatc acagtgagta aaggtcacgc
    12301 aacgctgggt ggcccgaata ccacctatgt gtttaaggcc ggtgaagaag cggttccgct
    12361 ggaagctggt caccgttttt aagtgaattg ccggatgcat gatccggcat cttgttcata
    12421 gtgcctgatg cgacgcttga cgcgtcttat caggcctaca ggattcacaa tattaatact
    12481 cgtcccgtcc ctcatcttca tcaggctgtt ccagcacact gtaagccacc gaacaaaaca
    12541 gcgagttcag acgctgcata tctcccagta agcctaaatg tagcgaactg gtttcaatgc
    12601 tttgcacgtt ttgctgatgc aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa
    12661 tgcgaaagcg atgcttgctg cggcgcaaac gacgagcgct ggtgacatcg ccagagaaga
    12721 acaccgacat tgccagcttt aaattactga gcaattgctc atagagcgca tccagttcct
    12781 tcaagccatc aagcgaaaat gctcgccttg cagccagcga tttgtcggca atttcactac
    12841 tcatgcgctc gacgatatcg gaggcctgtt caaggttgag cgacatttcg atgatctccg
    12901 cccagcggcg cgactcttct tctgccagtt cctcttttgg catccgcgcc agatacagct
    12961 taatggcggt atagagaacg ttgatatcat ctgccagctt acgcagctct ttctcctgcc
    13021 ttggctcgcc gtgcatcact ttattcagcc cttccatcat ctgttccatg gcgtcgccaa
    13081 tgcgcagggt ttcgcgcgcg gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga
    13141 catccagatg tttaggccgt agctgggtat ccagttccgg ttcatcgcga ataatcgttt
    13201 tgcaaaaccg tgccatcggg tcaacaaatg gcagcatggc caggcaacgt acaaggttgt
    13261 agaagacgtg gaaatagatc accagttccg cttttggcaa tgacaacttc cccattgtct
    13321 ctgccagcaa atggacaaac ggcaggataa tcaggctacc caccagctta aacagcagac
    13381 tacccagcgc gacacggcgg gctgcggcat tggcggcact gttgttgagc atcgccagca
    13441 ggccggaacc gaggttagca ccaatcacca gacagagcgc cacggggaag gagataatgc
    13501 ctgcggcggt cagagtcgcg gtcagcagta cagcagcaag gctggagtag ctgataatgg
    13561 cgaacatcgc gccaatcagc gcatccagca gaatatcgcc ggtcagcgag gcaaagatca
    13621 cctgaacgcc gtttgcctgg gtgatcggcg ttacggcctg cacaatcaac tccagcgcca
    13681 gcaaaatcag cccaagacca ataccgacgc ggcccagttg cccggcgcgt gactgtttgc
    13741 gtccgaggaa aaaaatcacg ccgataaaaa taagtaacgg tgacagccag gataagtcga
    13801 aggtgagaat acgcgccatt agcgccgtcc cgacatctgc ccccagcaca atgaccagag
    13861 ccggtgcgag ggctaccaga tcctgagcga cgaacgaggt caccagcatg gtggtggcat
    13921 tactgctctg taccagtgcg gtaacgccga tccccgcgca aaaggcgagc ggcttttttt
    13981 cgacgctccg gctaaggaca gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc
    14041 gaacaatatg agtcccccag accagcaggg cgacggcaga aagcaggtta agcagcgtta
    14101 acacgagttc aggttctcct tatcgttata tgttcctgaa gcaattgaat gaccaaagga
    14161 ccggaaacac gctgactggc atctttactg tgccttttga cattcgtttt tccggctaag
    14221 attattccag cctgtttcca gtataagtgt ttcatattaa taaagagaca gggcactcat
    14281 tgagcgccct gtttgttgta aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc
    14341 ccagattcgg tgccagccag cgctcaacat cggagacgct cataccttta cggcgggcat
    14401 agtcttcaac ctgatcgcgc tgaatttgcg ccaccgcgta gtacttgctg tccgggtggc
    14461 tgaaatacca acccgaaacc gatgcaccgg gccacatggc gaaagattct gtgagtttca
    14521 tgccggtatg tttttccact tccagcagct cccagatggt ggctttttcc gtatgttccg
    14581 ggcaggccgg gtagcccggt gccggacgga tgccctggta gttttcgcgg atcagttctt
    14641 cgttgctgag gttctcgttc ggcgcatagc cccagtagac tttacgcaca cgctcatgga
    14701 gatactccgc aaaggcttcg gctaaacggt cggcaagcgc tttcaccatg attttgttgt
    14761 aatcatcgtg ctgcgcttca aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca
    14821 cggcaaatgc gccgatgtaa tctgctttac cagaaagctt cggcgcaacg aagtcagcga
    14881 gacagtagtt agcgaatccc gttttttcgg tctgttgacg cagatggtgg ctgacgttga
    14941 tcacatgggt gcgcgtttcg tcacggtaga tttcaatgtc atcgcccaca cggtttgccg
    15001 ggaacaggcc caccacgcca cgcggattca gcgttttctc ggcgcttaat ttatccagca
    15061 tgtcgttggc gtctttaaac agccgctgcg cctcaacgcc caccacttca tcttccagaa
    15121 tgcgcggata cttcccggcc agcgaccagg tcataaagaa cggtgtccag tcgatgtaat
    15181 tacgcagagt ttcgatgctg gcttcaactt cctgcacgcc gagacggtgc gccaccggcg
    15241 gcgtgtaagc ctgccagtca aaagcgaagt cgttatcgcg cgccgcttcc agcgtgaccg
    15301 gtggtgtgcg tggtttcttg cgcccgtgct gaatacgtac ggtttcgtac tccttgcggg
    15361 tacgagcgac aaaatcatcg cgctgagtat cggaaagcag cgccgccacc acaccaacgg
    15421 tgcgcgaggc attctgcaca tacaccgtag ggccgctgta gttctgctcg attttcaccg
    15481 ccgtgtgcgc ttttgaggtc gtcgcgccac cgatcagtaa cggaatggtg aagccctgac
    15541 gctccatctc tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa
    15601 gtccaatcag atcagcattc acttctttag cggtacggag aattttttcc gcaggcacca
    15661 taacgccgag atcgacaatt tcgtagttgt tacattgcag caccacacca acgatatttt
    15721 taccgatgtc gtggacgtcg cccttcacgg tggcgatcac catcttgccg ttggttttgc
    15781 cctgctcttt gctggcttca ataaacggtt cgaggtaggc cactgcctgc ttcatcacac
    15841 gcgccgattt gaccacctgt ggcaggaaca ttttcccttc gccgaacaga tcgccgacca
    15901 cgttcatgcc gtccattagc gggccttcaa tcacttcaat cgggcgcgta gcctgctggc
    15961 gggcttcttc ggtatcctgc tcgataaact cagtaatgcc tttgaccagc gagtattcca
    16021 gacgtttatt cacttcccac gagcgccact ccgcctgctg ggcgttagcg gtgtcgtcgg
    16081 ttttgctgcc gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcatcgcgac
    16141 gattaagaat cacatcttcc accgcgtcgc gcagttcagc gggcaggtcg tcgtaaatcg
    16201 ccagttgccc ggcgttgacg atccccatat ccatgccatt gcgaatagcg tagtagagga
    16261 acaccgcgtg aatggcttcg cgcaccggat cgttgccacg gaatgagaaa gaaacgttag
    16321 atacgccgcc ggaaatcagc gcgtgcggca gttcgcgttt gatgtcttcg caggcgccga
    16381 taaagtcctg cgcatagttg ttgtgctctt caatgccggt ggcgaccgcg aagatgttcg
    16441 ggtcgaagat gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc
    16501 gacggcaaat ctcaattttc cgtgcgcggg tatcggcctg cccctgttcg tcaaaggcca
    16561 ttaccaccac cgctgcaccg taacgacgca acagtttcgc atgatggata aaggcgtcga
    16621 cgccctcttt cattgagata gagttaacaa tgcctttgcc ctgaatacac ttcagcccct
    16681 tttcgatgac ctcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt
    16741 caccggcaat cagattgaga aaacgcacca tcgccgcttc ggcatcgagc atcccttcat
    16801 ccatgttgat atcgataatc tgcgcgccgt tttccacctg ctgacgcgct acatccagcg
    16861 cctcgctgta tttctcttct ttgatcaggc gcttgaactt agcggaaccg gtgacgttgg
    16921 tgcgttcacc cacgttcaca aacaggctat cgtcgccaat gttcagcggc tccaggccgg
    16981 acaaacggca ggctacggga atttcaggca gtttgcgcgg cgctaatcct tctactgcac
    17041 gactcatcgc tgcaatatgt tgtggcgtgg tgccacagca gccgccgacg atattgagaa
    17101 aacccgcttg cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtact
    17161 caccaaaggc gttgggtagc ccggcgttcg ggtgcgcggt gacgtagcat tccgcaatcc
    17221 gtgatagttc ctgcacgtac tggcgcagtt catctggccc cagcgcgcag ttgaggccaa
    17281 aagtcagggc ttcggcatgg cgcagggagt tgtaaaatgc ttcggtggtc tgtccggaga
    17341 gcgtgcgccc ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccta
    17401 acgcttcgaa ctccgttttc accgcaaaga ccgccgcttt ggcgttaagg gtgtcgaaaa
    17461 cggtttcaat cagaatcaga tccgcaccgc cttccactaa tgctttggtg gattcgcgat
    17521 aggccgccac cagctggtca aaagtgatgt tgcggaatgc cggatcgttc acgtccggag
    17581 aaatggacgc cgtgcggttg gttgggccga gaacgccggc aacgtagcgc ggtttctccg
    17641 gcgtgcgcgc ggtccactcg tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga
    17701 tttccgccga cagggattcc atctggtaat ccgccatcgc aatggtcgtg gagttgaagg
    17761 tgttggtttc gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca
    17821 cttccggttt gcttagcacc agcagatcgt tattgccttt gaggtcgcat ggccagtcgg
    17881 caaagcgttc accacgaaaa tcggcttcgt tcagtcgata gctctggatc atggtgccca
    17941 taccgccgtc cagcaccaga atacgttcat ttaactgcgc acgcagttgt tccactttgc
    18001 tgctcacact tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac
    18061 tggcataaac gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt
    18121 aactcatcgg atcagttcag caactattgc attagctaac aataaaaatg aaaatgattt
    18181 ccacgataca gaaaaaggag actgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa
    18241 aacccgccgt tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga
    18301 aattactgga gtggattgcc gaatccaatg gcagtgtggc actcacggag ctggcgcaac
    18361 aagccgggtt acccaattcc acgacccacc gcctgctaac cacgatgcaa cagcagggtt
    18421 tcgtgcgtca ggttggcgaa ctgggacact gggcaatcgg cgcacatgcc tttatggtcg
    18481 gcagcagctt tctccagagc cgtaatttgt tagcaattgt tcaccctatc ctgcgcaatc
    18541 taatggaaga gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag
    18601 cgattattat cgaccaggta cagtgtacgc atctgatgcg aatgtccgcg cctatcggcg
    18661 gtaaattgcc gatgcacgct tccggtgcgg gtaaagcctt tttagcccaa ctgagcgaag
    18721 aacaggtgac gaagctgctg caccgcaaag ggttacatgc ctatacccac gcaacgctgg
    18781 tgtctcctgt gcatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg
    18841 acgatgagga acatgcgctg gggctacgtt gccttgcagc gtgtattttc gatgagcacc
    18901 gtgaaccgtt tgccgcaatt tctatttccg gaccgatttc acgtattacc gatgaccgcg
    18961 tgaccgagtt tggcgcgatg gtgattaaag cggcgaagga agtgacgctg gcgtacggtg
    19021 gaatgcgctg actttttctg gcgggcagag gcaatattct gcccgtcata cctgagtggt
    19081 aatagaataa gggtgtctgt taatcgcatt gacgtcaaaa taacttattg tcatacactt
    19141 catacacttc atacactttt attaactcag cattattttt aaacatcaaa ccacttaatt
    19201 attaacaaaa tcgcaagatc tcattccacc gaaactttta cggctgcatt atgagattg
//
LOCUS       DAZFBR010000088        19244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFBR010000088 DAZFBR010000000
VERSION     DAZFBR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.088"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            12..530
                     /gene="yfgH"
                     /locus_tag="ACHJZ2_003448"
     CDS             12..530
                     /gene="yfgH"
                     /locus_tag="ACHJZ2_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4569497.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            546..1085
                     /gene="yfgI"
                     /locus_tag="ACHJZ2_003449"
     CDS             546..1085
                     /gene="yfgI"
                     /locus_tag="ACHJZ2_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO4569498.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1178..2755)
                     /gene="guaA"
                     /locus_tag="ACHJZ2_003450"
     CDS             complement(1178..2755)
                     /gene="guaA"
                     /locus_tag="ACHJZ2_003450"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO4569499.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRSVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2824..4290)
                     /gene="guaB"
                     /locus_tag="ACHJZ2_003451"
     CDS             complement(2824..4290)
                     /gene="guaB"
                     /locus_tag="ACHJZ2_003451"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO4569500.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4452..5822
                     /gene="xseA"
                     /locus_tag="ACHJZ2_003452"
     CDS             4452..5822
                     /gene="xseA"
                     /locus_tag="ACHJZ2_003452"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO4569501.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5819..6034)
                     /gene="yfgJ"
                     /locus_tag="ACHJZ2_003453"
     CDS             complement(5819..6034)
                     /gene="yfgJ"
                     /locus_tag="ACHJZ2_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO4569502.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6103..7575)
                     /gene="der"
                     /locus_tag="ACHJZ2_003454"
     CDS             complement(6103..7575)
                     /gene="der"
                     /locus_tag="ACHJZ2_003454"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO4569503.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7693..8871)
                     /gene="bamB"
                     /locus_tag="ACHJZ2_003455"
     CDS             complement(7693..8871)
                     /gene="bamB"
                     /locus_tag="ACHJZ2_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO4569504.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8882..9502)
                     /gene="yfgM"
                     /locus_tag="ACHJZ2_003456"
     CDS             complement(8882..9502)
                     /gene="yfgM"
                     /locus_tag="ACHJZ2_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO4569505.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9520..10794)
                     /gene="hisS"
                     /locus_tag="ACHJZ2_003457"
     CDS             complement(9520..10794)
                     /gene="hisS"
                     /locus_tag="ACHJZ2_003457"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO4569506.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10905..12023)
                     /gene="ispG"
                     /locus_tag="ACHJZ2_003458"
                     /gene_synonym="gcpE"
     CDS             complement(10905..12023)
                     /gene="ispG"
                     /locus_tag="ACHJZ2_003458"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO4569507.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12050..13063)
                     /gene="rodZ"
                     /locus_tag="ACHJZ2_003459"
     CDS             complement(12050..13063)
                     /gene="rodZ"
                     /locus_tag="ACHJZ2_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO4569508.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13348..14502)
                     /gene="rlmN"
                     /locus_tag="ACHJZ2_003460"
     CDS             complement(13348..14502)
                     /gene="rlmN"
                     /locus_tag="ACHJZ2_003460"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO4569509.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14652..15083)
                     /gene="ndk"
                     /locus_tag="ACHJZ2_003461"
     CDS             complement(14652..15083)
                     /gene="ndk"
                     /locus_tag="ACHJZ2_003461"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO4569510.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15224..16078)
                     /locus_tag="ACHJZ2_003462"
     CDS             complement(15224..16078)
                     /locus_tag="ACHJZ2_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4569511.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
     gene            complement(16078..16899)
                     /locus_tag="ACHJZ2_003463"
     CDS             complement(16078..16899)
                     /locus_tag="ACHJZ2_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFO4569512.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16892..17521)
                     /locus_tag="ACHJZ2_003464"
     CDS             complement(16892..17521)
                     /locus_tag="ACHJZ2_003464"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFO4569513.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17518..>19244)
                     /locus_tag="ACHJZ2_003465"
     CDS             complement(17518..>19244)
                     /locus_tag="ACHJZ2_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFO4569514.1"
                     /translation="TLLDTKLVILWGHNPTETIFGHTNYYFQKMKQNGTRFIVVDPRY
                     SDTVSSLADQWIPLLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVP
                     ANESLVAYLFGAKDGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRH
                     ICGERTARGSTLLASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNW
                     MQAADDASKVTPQDGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIE
                     FIVLSDLFMTPSAKYADVLLPETSFMERWNIGETWGTASYLILSEKLIEPDFERRTDY
                     DWLRDVAKKLGVEAEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKS
                     APHIAFEANIRDPQNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLT
                     AKYPLQLITWKGKNRANSTQYANPWLQEVQTQKLWLNPQDAKQRGISEGDSVKIYNDR
                     GVSIIPVEITPRIIPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQT
                     MLVEVEKA"
BASE COUNT         4756 a         5229 c         4686 g         4573 t
ORIGIN      
        1 aacaaggaat agtgatgaaa tttaaaaaat gtcttctgcc tgtggcaatg ttagcgtcat
       61 tcactctggc aggatgccag tcaaatgctg acgatcatgc cgccgatgtt tatcaaaccg
      121 atcaactgaa taccaaacaa gaaactaaaa ccgttaatat tatttccatt cttcccgcaa
      181 aagttgccgt agacaactcc caaaataaac ggaacgcaca agccttcggc gcgcttattg
      241 gcgcagtcgc tggcggtgtt atcggccaca acgtcgggtc tggcagcaat tccggaacga
      301 cggcaggtgc agttggcggc ggagctgtag gcgcggcagc gggttctatg gtgaatgata
      361 aaaccttagt ggaaggtgtt tctttaacgt ataaggaagg caccaaagtg tatacctcta
      421 ctcaggtggg taaagagtgc cagtttacga caggtttagc cgttgttatt accacgacgt
      481 ataacgaaac gcgtattcag ccaaatacca aatgtcctga aaagagctaa taatcaggag
      541 gagtcatgaa gaaagttttt ctttgcgcca tcttagcctc cttaagctat ccggctatcg
      601 cctcatcatt gcaggatcaa ctctctgctg tcgcagaagc ggaacagcaa ggtaaaaatg
      661 aagagcaaag gcagcatgac gaatgggtcg cggagcgcaa cagggaaatc cagcaagaga
      721 agcaacgtcg cgcaaatgcc caggctgccg ctaacaaaag agcggcaacg gcagcggcaa
      781 ataagaaagc tcgtcaggat aaactggacg ccgaagccac tgcggacaaa aaacgcgatc
      841 aaagttatga agatgagcta cgcagcttag agattcagaa acaaaaactg gcgctggcaa
      901 aagaagaagc ccgcgttaag cgagaaaacg aatttatcga tcaggagctg aagcacaaag
      961 ccgcgcaaac tgatgtcgtg caatctgaag ctgacgccaa cagaaatatg actgaaggcg
     1021 gtcgcgatct gatgaaaagc gtgggcaaag cagaagagaa taaatcggac agctggttta
     1081 attaatcgat gttagtaact tcaagcctat aattcttgaa gataaaaaac cctctgtagt
     1141 aacagagggt tttgttcatt catagtgcag ggtcaaatca ttcccactca atggtagctg
     1201 gtggcttgcc gctgatgtca tacaccacgc gggaaatacc gttcacttca ttgataatgc
     1261 ggttggaaac gcgaccgagg aaatcgtacg gcagatgcgc ccagtgtgcg gtcataaagt
     1321 cgatggtttc gacagaacgc agagagacaa cccagtcata cttacgacca tcgcccatta
     1381 cgccaacgga acgtactggc aggaagacgg tgaacgcctg gctgactttg tcgtacaggt
     1441 ccgctttacg cagttcttca atgaagatgg cgtcagcacg gcgcagcagg tcacagtact
     1501 ctttcttcac ttcacccaga acacgaacgc caaggcctgg tcccgggaac gggtgacggt
     1561 acagcatgtc gtacggcagg cccagctcca gaccaatctt acgcacttcg tctttgaaca
     1621 gctctttcag cggttcaacc aggcccatct tcatctcttt cggcaggccg cccacgttgt
     1681 ggtgagattt gatgacgtgt gctttaccgg ttgcagacgc cgcagattcg ataacgtcag
     1741 ggtagatggt gccctgcgcc agccacttca cgtcttccag tttcagcgct tcttcatcga
     1801 atacttcaac gaaaacgcga ccgatgattt tacgttttgc ttccggatcg ttttcgccag
     1861 ccagcgctga caggaagcga tcttctgccg gtacgtgaac aatgttaaga ccaaagtgat
     1921 cgccaaacat atccagaacc tgctctgctt cgttgagacg cagcaggccg ttgtcgacga
     1981 atacgcaagt caggttttta ccgatagcgc ggtgcagcag cattgcggtt acggaggaat
     2041 ccacaccacc agagaggccg aggatgactt tatcgtcgcc tacctgctcg cggatgcgag
     2101 ctacagcatc gtcgataatt ttcgctggcg tccacagggc ttcacactgg cagatatcac
     2161 gcacaaaacg ctccagcatg cgcataccct ggcgggtgtg agtcacttcc gggtggaact
     2221 gtacgccata gaagcgtttt tcttcgttag ccataatggc aaacgggcag ctttcggtgc
     2281 tggctacggt gacgaagtcg gacgggatag cggtaacttt atcgccgtgg ctcatccaga
     2341 catcgagcag cggtttaccg tctgcggtca gcgcatcttc gataccgcga accagtgcgc
     2401 tgtcgtttac gacttcaacc tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt
     2461 gaccgcccaa ctgcattgcc atggtctgca tgccatagca aacgccgaat accggtacgc
     2521 ctgcttcaaa gacatactgc ggcgcacgcg ggctgttttc ttcagtagta ctttccgggc
     2581 cgccggaaag aataatgccg cttggattga agtcacgaat ttgtgcttct gtcacatccc
     2641 atgcccacag ttcgcagtaa acacccagct cacgcacgcg gcgcgcaacc agttgagtgt
     2701 actgagaacc gaagtccaga atgaggatgc gatgcttatg aatgttttcc gtcattgacg
     2761 cttattccga ggcaagtgaa acagataata taaatcgccc gacatgaagt cgggcgaaga
     2821 gaatcaggag cccagacggt agttcgggga ctctttagta atggtcacgt cgtgaacgtg
     2881 gctttcctga atgcccgcac cgctgatacg tacaaactcc gctttagtac gcagttcgtc
     2941 gatagtacca cagccggtca aacccataca ggagcgcagg ccgcccatct gctggtgaat
     3001 gatctctttc aggcgacctt tataagctac gcgaccttcg ataccttccg gcaccagttt
     3061 gtcggcagcg ttatcgctct ggaaataacg gtcagaggaa cctttggaca tcgcgcccag
     3121 ggaacccata ccacggtaag atttgtaaga acggccctgg tagagttcga tttcacccgg
     3181 agattcttca gtacccgcca gcatggaacc taccatcacc gcgcttgcgc cagcggcgat
     3241 agctttggcg atgtcgccgg agaagcgaat accaccatct gcgataaccg gaataccggt
     3301 gccttccagg gcttcaactg cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt
     3361 aacgatacga gtcgtacaga tagaaccagg gccgataccg actttaaccg cgctgcaacc
     3421 agcttctgcc agagcgcgtg cacctgcagc tgttgccacg ttgccgccga tgatttgcag
     3481 atccggatat ttagcacggg tttcacggat acgctgcaaa acgccttctg agtgaccgtg
     3541 ggaggagtcg atcagcagaa cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc
     3601 gttacccgca cctgcgccaa ctgcagcacc aacacgcaga cggccttgct cgtctttaca
     3661 ggcgttcggt ttacgttccg ctttctggaa atctttcacg gtgatcatgc cgatcaggtg
     3721 gaattcgtca tcaaccacca gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac
     3781 cacttcacgg gcttcacctt cacgcacggt gaccagacgc tctttcggcg tcatgtaaac
     3841 gctaaccggc tggttcaggt cggtaacaaa acgcacgtca cgaccggtga taatccccac
     3901 cagttcgttt tcttcggtaa cgaccggata gcccgcaaaa ccgttacgct cggtcagttc
     3961 tttcacttcg cgcagcgtcg tggttggcag aacagtctgc ggatcagtca ccacaccaga
     4021 ttcgtgtttt ttcacacggc gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg
     4081 gataaagccg ataccgcctt cctgagccag agcaatagcc aggcgcgctt ccgttacggt
     4141 atccattgct gcggaaagca tagggatatt cagacgaata gttttcgtca gctgggtgct
     4201 gaggtcagca gtattcggca gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa
     4261 cgtcagagct tctttagcga tacgtagcat gggcaatatc tcgaccagag tggttaataa
     4321 atattgccgc ggcattatac agagcgtaac cgattgcatc tacccctttt tgcaaaaaat
     4381 gcttgctatc cccgaagggc gggttactat cgactgaata acctgctgat ttagaatttg
     4441 atctcgctca catgttacct tctcaatccc ctgcaatttt taccgttagt cgcctgaatc
     4501 aaacggttcg tctgctgctt gagcatgaga tgggacaggt ttggatcagc ggcgaaatct
     4561 ctaatttcac acaaccggct tccggtcact ggtactttac actcaaagac gacaccgccc
     4621 aggtacgctg cgcgatgttc cgcaacagca accgccgggt gaccttccgc ccacagcatg
     4681 ggcaacaagt tttagttcgc gccaatatta cgctctacga gccgcgcggc gactaccaga
     4741 taatcgttga gagtatgcag ccggccggtg aagggctgct gcaacagaag tacgaacagc
     4801 tcaaagcgaa gttgcaggct gaaggattgt tcgatctgca atacaaaaaa ccacttccct
     4861 cccctgcgca ttgcgttggt gtgatcacct caaaaaccgg tgctgcgcta catgatattt
     4921 tgcatgtgtt aaaacgtcgc gatccttctc tgccggtgat catctacccc accgccgttc
     4981 agggcgatga cgcgccgggg caaattgttc gcgccattga actggcgaat cagtgcaatg
     5041 agtgcgacgt gttgatcgtt gggcgcggcg gcggttcgct ggaagattta tggagtttta
     5101 acgacgaacg cgtagcgcgg gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg
     5161 ggcatgagac ggatgtgacc attgccgatt ttgttgccga tctgcgtgca ccaacaccgt
     5221 cggctgccgc cgaagtggtg agccgtaatc agcaagagtt actgcgccag gtgcaatcga
     5281 cccgtcaacg gctggagatg gcgatggatt attatctcgc caaccgcaca cgtcgcttta
     5341 cgcaaattca tcaccgatta cagcaacagc atccgcagct ccggctggca cgccagcaaa
     5401 ccatgcttga acgcctgcaa aaacggatga gctttgcgct ggaaaatcaa cttaagcgtg
     5461 ccgggcaaca gcagcagcga ttaacacagc ggctgaatca gcaaaatcca cagccgaaga
     5521 ttcatcgcgc gcaaacgcgc attcagcaac tggaatatcg tttagcagaa accctgcgcg
     5581 cacagcttag cgccacgcgt gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa
     5641 gcccactgtc aacgctggcg cgtggataca gcgttactac tgctactgac ggcaaggtac
     5701 tgaaaaaagt gaagcaggtt aaagcgggtg aaatgctaac cacacgtctg gaagacggct
     5761 gggtagaaag tgaagtaaaa aacatccagc cggtaaaaaa atcgcgtaaa aaagtgcatt
     5821 aagccagcac gaattcaacg cgttttttcg atatcaagcc atgcccgtgc tgacagaagt
     5881 aatctaccgc accgcaggct tttaacacct gcaacggttg atggcagtcc gggcaaagag
     5941 ctttcatttc aataactttc ccgcaggatg ggcaatacgc atggccgtta tcctgatcaa
     6001 gcacatgctg acattgtgga caatgcagtt ccatatgaat tcctccggta gccacccctc
     6061 tacattcata gagggaatgg cagataaaat acttacggat aattatttat ttttcttgat
     6121 gtgcttcatc agacgcttac gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt
     6181 cgcatacggg ttttcccctt ctttgaactg aatacgaatc ggcgatccca ttacgtccag
     6241 cgatttgcgg aagtagttca tcaagtaacg cttgtaggaa tcaggcaggt ctttcacctg
     6301 attaccgtga atcaccacaa tcggcggatt ataaccaccg gcgtgtgcat atttcagctt
     6361 cacacgacga ccgcgtacca gcggcggttg gtgatcttca acagccatcg tcatgatgcg
     6421 cgtcagcata gaggttccca cacgacgggt ggagctgtca tacgcttcac gtactgattc
     6481 aaacaagtta ccaacaccac tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc
     6541 gataaagccc agacggaagt ccagcgtttc tttcacctgc tctttcactt cctgactcag
     6601 gccatcccac ttattcacca caatgacaag tgagcgccca ctattgagaa taaagcccag
     6661 cagcgagaga tcctgatcgg aaataccttc gcgcgcatca atcactaaca tcaccacgtt
     6721 ggcgtcttca atggcctgca acgttttgat tacggagaat ttctctacag catcggtgat
     6781 tttgccgcgt ttacgtacgc cagcggtgtc aatgagcaca tactcacgtc catcgcgttc
     6841 cattggaatg tagatactgt cacgcgtcgt accaggcatg tcataaacaa caacgcgctc
     6901 ttcaccaaga atacggttag tgagtgtaga cttacctacg ttcggacgac ccacaatcgc
     6961 cagtttgatc ggcagacttt gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc
     7021 ttccgcttca aattgcgccc agtattcagc gtcttcgtcg acttcctctt gcggtgcgag
     7081 atcttccatc cacggcagta gcacatgctc cagcagactt aatacgccac gaccgtgaga
     7141 cgcggcgatc gggtagattt cacctaaacc aagcgagtag aaatcaacca ctgcctgatc
     7201 gggatccaga ccgtcagttt tgtttgccac caggaaggtc ggtttttcac gggagcgcag
     7261 atgtttggca atcgcttcat ctgccggcat caggcccgcg cgcgcatcca ccataaacag
     7321 tacgacgtcc gcttcttcaa tcgccagcag cgactgttcc gccatgcggg tttctacacc
     7381 gtcttctgtg ccatcaatcc cgccggtatc aatacagata aactcacggc cttcaatttc
     7441 cgcacgaccg tacttacggt cacgagtcag acccgggaaa tccgcaacca gcgcatctcg
     7501 ggtgcgagtt agacggttaa ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc
     7561 gaccacaggt accatgttta aagcctcatt tttataaatc atcagacaac gcacgctata
     7621 ttcgcgtcgt tgttaaaaac aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa
     7681 cgacagagac gattaacgtg taatagagta cacggttccg tcttttgcct ggatcagcag
     7741 tttgccgtca gcggcaaccg gttcagtctg gaaaccggaa ctatcaactt tttgctgggc
     7801 aacgaaacga ccatcttcga cgttaatcca gtgcaaataa ccttcactgt caccgaccac
     7861 caggttgcca ttatacagca ccggagaagt cagcaggcga tgcagcagat cgctttgtgt
     7921 ccacagcgta acgccgccat caatggtcaa cgccatcacc cggtcatttt gatcgaccag
     7981 atagatgcga ttgccgtcga cgatgaaatc attcaccgaa cccagttcgc gtttccacat
     8041 aatctgacca ctgcgcagat caagcgccgt caggttacca ttataggcca gcgcgaaaac
     8101 aacgccgtta acaacggcgg gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga
     8161 accggtcgct tgggaaatac gctgctgcca aatcatctgg ccctgttcca tcagcactgc
     8221 gctgacgcga ccattatcgc cccccacgac ggccgcacca aaagccgttg ccggcgcaga
     8281 ctcgccacgc aaagagagcg aaggcatatc gaggttaact gtccatttga cagcgccgtc
     8341 agcttcgttc agcgcttgta actgaccgtt actggtgtgg attaacacca gaccgtcgct
     8401 gaccaccggg cgcgaaagtg cttcacccgc gactttagtt tgccatgcca cagtaccatc
     8461 gctggtattc agcgcgtaaa cctgcgcctt ttcgctgcca atgtagacat gtccaccaga
     8521 cacggtcaca ccgccagaaa gtaatgcagg ctctttagag aaccagccat ctttctcggc
     8581 caggctgaca gaccagattt ctttgccatc atccgcattc agcgctttta ctaaaccagc
     8641 gcggtccgct gcatagacaa cgttgtccgc cagtgccgga tgaagattgg aatagaagtt
     8701 gccaatgccg ctaccaacgg aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac
     8761 ggttggcaat ggggacatct ttaccacatc ttcttcgctg ttaaacagcg aacagccgct
     8821 taaaagggta acggaaagca gtcctggcag cagtaattta cgcaattgca tcgggtccct
     8881 ctcagatgga caaattatta attttcatct gcatcatttc gctcagtgcc ggagtaacat
     8941 cgcttttcac gcctgcttcc catgcactac gcgcaccttg cttatcacct ttgctcagca
     9001 atgcttcacc acgcaggtcg gcaacaatgg cagcccaccc ttcacctttg atggtatcaa
     9061 gggttttcag cgcggcatca gcctgcttga gctgtacctg aacgcgagca agacgcagat
     9121 ttatcacggc tttgagattt tcatcgctcg tgtctgccag cccctgttgt aactgggcgg
     9181 cagctttctc tagttcattt ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg
     9241 caccataagt atttttattt tcagcagcaa atttttccgc cgccgggatg ctatccggtt
     9301 tgccttcgct cactgcggta accgcatttt gataggcaag agaagcggag cgtgcagaat
     9361 caacctgatg gctgttccag tagcgccagc caatcagtgc gccaacgccc aaaatcaccc
     9421 caacagccag tgctttgcca ttttcagcaa aaaagcgttt aatcgcttct acctggtcgt
     9481 tttcgttctc gtaaatttcc acgctgtcct tctccttcct taacccagta acgtgcgcaa
     9541 atgcgcggct acgctatcct gcgcaactgc cgtttgctca ccagagcgca aatccttcac
     9601 tactgctgtg ccgttagcca cttcagactc acccagcacc acagcaacgc gggcacccca
     9661 tttatcagca cgggcaaact gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt
     9721 cacgcccggt aattcatcac gcagacgctc agctaatgcc atagccgcag attgtgtatc
     9781 agcacctgaa gccaccaggt atatatcgac aacaggatcg gctttaaatt ccggattaac
     9841 ggcctgtact aacaatacaa gacgttcgag gcccatcgca aaaccgacag ccggtgttgc
     9901 acggccgccc agttgttcca caagaccgtc ataacgaccg cctgcacaca cggtgccctg
     9961 ggagccgaga ctgttagtca cccactcgaa aacagtacgg ttgtagtaat ccagaccacg
    10021 caccagacgc tggtttacgg tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc
    10081 ggcaaaatgc tcacgagatt cctcgtccag ataatcacct aatgccggag cgtcgttgag
    10141 aagcgcctgc acttccggat tttttgaatc cagcacgcgc agcgggttag tgtacatgcg
    10201 gcgtttgcag tcttcgtcca gcttttcttt atgctgctca aggaatgcca ccagcgcatc
    10261 gcggtaattg gcgcgtgctt ccagcgaacc gatagaattc agctcaagag ttacgtgttc
    10321 ggaaataccc agcgcgcgcc accagcgggc ggtgagcata atcagttcag cgtcgatatc
    10381 cggaccttgc agaccgaaaa cttcgcagcc caactgatgg aactgacgat aacgcccttt
    10441 ctgcggacgc tcgtgacgga acatcggccc gatataccac agacgctgtt cctgattgta
    10501 cagaagacca tgctcgatgc cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt
    10561 caggctgtcg ccattgcgat cctcaaaggt gtacatctct ttttcaacca cgtcggtgac
    10621 ttcaccaatc gcacgtttga atagcggggt ctgctctaca atcggcaagc ggatttcact
    10681 gtaaccgtag ctgccgagca cgtttttcag tgtgccttca atgcgctgcc agatggccgt
    10741 ttcgccaggc aggtaatcgt tcatgccgcg aatggcttga atgttttttg ccacgtttat
    10801 tctctttctg aatataaaaa tgaaccctca acgcttccct caatgtttcg ggagccatgc
    10861 gggttcaatc atacacggga agcgaggcgc ttcccatcac gttattattt ttcaacctgc
    10921 tgaacgtcaa ttcgacgcgc ttcgtccagc tgactggctt tcgcacgaat gcgtgcctcc
    10981 agctggtcga tcatatcgtt gttgtccaga cggtctttgc gcacgccatc ttcatagaga
    11041 ccgcttttct tgttgccgcc ggtgacgccg agtgtagaaa ccagcgcctc acccgggcca
    11101 ttcaccacgc agccgataat cgaaacgtcc atcggagtga tgatatcttc caggcgttgc
    11161 tccagcgcgt taactgtacc gataacatca aattcctgac gcgaacaggt cggacaggcg
    11221 atgaagttga tacctcgcga acggatacga agcgatttca aaatatcgaa accgactttg
    11281 atctcttcga ccggatcggc cgccagtgat acgcgcagcg tgtcgccgat gccttcagac
    11341 agcagcagac ctaaaccaat ggcggatttt actgccccgc tgcgcgcgcc acccgcttcg
    11401 gtgatcccca gatgcagcgg ctgatcgatt tgttttgcca gcaaacgata agactcaaca
    11461 gcgaggaaga cgtcagacgc tttcacgctg actttgaact gatcgaagtt caggcgatcg
    11521 agatgatcaa catggcgcat ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac
    11581 ttttcttgca gatctttttc cagcgatccg gcgttaacgc caatacggat cggaatgttt
    11641 ttatcgcgcg cacagtcaac caccatgcga atacgctctt cattaccgat attgccaggg
    11701 ttaatacgca gacaatcgac gccgtattcc gctactttca gcgcaatgcg atagtcgaag
    11761 tggatgtcag ccaccagcgg cacgttaacc tgctgtttga tgagtttgaa cgcttctgcc
    11821 gcgtccatcg tcggtacgga gacacggacg atatcagcgc caacgcgttc cagcgccttg
    11881 atttgattga ccgttgcttc gacatctgtc gtacgcgtat tggtcatgga ctgtacggcg
    11941 atgggagcac catcgccaat cggcacattc ccaacgtaaa tacgtgttga ttttctacgt
    12001 tgaattggag cctggttatg catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg
    12061 gtgattgttc ggcattgagg gtcagacgcg caacctggtt agttctgata aaacgactca
    12121 gatcgacagg tttcccttga tactggatct gtactgcggc tggcgcacca attttcagtt
    12181 tgtacggtgc ctggccggtt aagttcaaat taccgtcttt acgctgcata ccgctaaaca
    12241 attttttacc ggtagcatca gtgacctcca gccagcaatc ggcagtaaag ttcatcacca
    12301 gcgcattcgg atcagccacc ggcgtggtca cgccagcctg atcggttggc aacggcgcag
    12361 caccatctgg cgttgttgct gccgttggtg ccggggtcgc ggcggtatca acatttgcct
    12421 gcgaaggcga aacaaccgca ttctgttgcg gatcaacagc tggtgctggc gcagttacga
    12481 caggtgtttg cgtgttggtt gcggtagtat ccacagacgc tggcggcgtg gacgttgtag
    12541 ccgggtctgt agttgtcgac gtatttaacg gaacactctg cccctgctcg ctattactgc
    12601 tcagttccgc cgaagattga tcggccatag tggtgatctc ttcctgctga gctttgtggt
    12661 cttgccacca ccaggcaccg ctcaggccga taaccacaaa caacaccagc caggtgaagg
    12721 tcatcagcca gccgtcgcgt tttttgcggc gtttaccgag ggaaaaactc tgcatcggcg
    12781 caacttttgc agcccgtagt ggagcctgct tttccagccc tggcagcagt tcttcttctg
    12841 gaatatgtac cagacgcgca taagagcgga tatatccgcg caggaatgtt gaagcaagat
    12901 cggcgggtgc cttatcttct tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct
    12961 cggcaacggc ctgctgacta agtcctagtt gttcgcgagc attacgcagg cgagcgccgg
    13021 tagtaagtgc ttcattttgg tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt
    13081 aaatgagaat tgagatgccg gtgaaccatc atgctcaccc acaccgcgaa acatccgtta
    13141 agttaaccgt tatcatacag tataagaccg tctgtgtgct cgtgacaaag cccacattca
    13201 ctaaggctaa cttactgttg catcgttaca tactgcctta aagtcagcaa aaacgcaccg
    13261 ttaatattga ccagacaatt gcaacttaat acttcattca taaaacatta cgccacggta
    13321 cataaagtaa ccgtggcgta atggctatca gaccgcttta atgtcgatgg cttcaccctg
    13381 catccgttta cgcagggtac gtttcgtacg gtcgataaca tcgcccgcca actgaccaca
    13441 ggcagcatcg atatcatcac cacgcgtttt acgcacaata gtggtgaaac cgtagctcat
    13501 cagcactttt gagaaacggt cgatacggct gttcgagctg cgtccatacg gcgcagccgg
    13561 gaacgggttc cacgggatca ggttgatctt acacggcgta tctttcagca gttccgccag
    13621 ttggtgcgcg tgttcagtgc cgtcgttaac gtggtcaagc atcacgtatt caatagtgac
    13681 tcggccctga ttggcgttgg atttctccag ataacggcgc accgcagcaa ggaacgtttc
    13741 gatattgtac tttttgttga tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg
    13801 cagggaaatt gccagcgcaa catcgatcat atcgcccagt ttatccagcg ctggaactac
    13861 accggaagtg gaaagcgtca cgcgacgttt agacagacca aaaccgaaat catcaagcat
    13921 gatttccatc gccggaacga cgttgttcag gttgagcagc ggctcgccca tgcccatcat
    13981 cactacgtta gtgattgggc gctgaccggt gacttttgct gcgccgacga ttttcgccgc
    14041 acgccacacc tggccaataa tttccgatac ccgcaggttg cggttaaagc cctgctgggc
    14101 ggtggaacag aatttacact ccagcgcaca ccccacctgc gaagagacgc agagcgtagc
    14161 gcggtcgtct tccgggatgt acaccgtttc gacacgctgg tcacccacgg caatcgccca
    14221 tttaatggtg ccgtcagatg aacgctgttc ttcaaccact tccggtgcgc ggatctccgc
    14281 cacctctttc agtttgccgc gcaacacttt gttgatgtcg gtcatctcat caaaattgtc
    14341 gcagcaatag tgatacatcc acttcatcac ctgatcggcg cggaagggtt tttcacctaa
    14401 atctttaaaa aactcccgca tctgctgacg attgagatcc agcaggttga tttttccatc
    14461 tttggtggtg acgttttcag gtgtgactaa ttgttcagac atatgctatt ccggcctcgt
    14521 tattacacgt tatggcccct ggagggttga aaaaagaaac gccccggtga gcggctgctc
    14581 gtccgggggc gctgcattgt acaaattctg gcgcacggat gccacgtttg cacgcggcat
    14641 ttacgaaatt attaacgggt gcgcgggcac acttcgcctt cgccgaagaa ataagcgatt
    14701 tcgcgagcgg cagattcgac ggaatcagaa ccgtgggtac cgttttcggt caggctgtca
    14761 gcgtaatcag cgcgcagagt accggccagt gcgtttgccg gattggtcgc gcccagcaga
    14821 tcgcggtgac gctgaacggc gttttcacct tccagcacgg aaaccacgat cgggccagag
    14881 gtcatgaatt caaccagacc atcaaagaac ggttttccat cgtgttcagc ataaaagcca
    14941 cgtgcctgtt caacggtcag gtgcagcatt ttggtgccga caattttgaa ccctgcagct
    15001 tcaaagcgcg caaagatatt accaatgacg ttttttgcta ccgcgttcgg tttgataatg
    15061 gaaaaagtac gttcaatagc catttttacc tctgtaaatt gttctgttgt tgtctgtacc
    15121 agcgtacgag atggcgcgga ttataatgag caacagagcc gttgactatt gatgaaggta
    15181 acattttttt aaaataaaat gagttttagc aacaactcga cttttatggt gttctcatcc
    15241 cataacgatg acgttggcaa taaagacaat tttgcgcatt ttgctgaaaa gtaagaaagg
    15301 atttttgaca ggttttacag actttgcgat tagcatgaag attcacaatt tttgccggta
    15361 gaatcgtcaa ttcaacggtc attgcctgat gaaaacaaac agcaatacaa ttttggcatc
    15421 cgttacacaa ggcatcattt atttgtaaat ggccttcgtt aagcgaaaag acctgttgct
    15481 cgcaggcccg ccagcatgca ccgcaaagct gacacttttg aggatccatc atcgggcgag
    15541 catcactaat ctggggccat aaataatgta accgacgtaa tcctggttgt atttgtgtca
    15601 tctgaacatc cgcacgctgc acatggaaca aacgacgccg ggaaacattc acctcactgg
    15661 tatgtggaga taccacagac cagccttcct cgccatattg ccgtaagcat tcgtttaaca
    15721 gcaataatgc gcgctcccat gccttcgcct gactcagatc aatcgcgatc gctgtgatcc
    15781 cctgctcttt atgccatagc aaaagttctt ccagcgtcgg cgcaatagtg gtgaaaggtg
    15841 cgaccagggt gtgattgcaa aaataacgta aaggggaatt aacgccacga atagcctctt
    15901 gcggacaaat aaactggcaa accgcacaat caatacattg ttccgcgtcg accgctatgc
    15961 cctcttctgt cagcgttagc gcctgtgccg ggcaagcctc gacacaggca tgacatgttg
    16021 caaaacgaaa gcgttggcgc gcgcattccg cgccaatcgt tatgttatga cttaacatta
    16081 gccgatacta aaaaagacaa aacgcagcat aacttcactc cccactagca ttacgctacc
    16141 gaataacgcc gccgggatat tctttttaca accaccgaca gcaaagaaca tcatcccaat
    16201 gacggaaatt ccccagctta gtgtcagtat gggttttaac tgtcccagca tcgctaatgg
    16261 cgcatgtggc aatgtggcta tctgcatcgc atttgccgtt aaatccccta tccacaacgg
    16321 ctgaaccaac aaacgcagca gaaccaggag agcgatcacc aacacgcatc cattacgcag
    16381 ggcatcaatc tgcggcaaaa ttccgctaat acgaaacact gctaccagcg ctgaaccgag
    16441 aattcccact gtacctaaga acataatcag cgtgttgtaa tgctgccagg tgataatcga
    16501 cgtatggatg taaacttgcg ccatgcagaa aacatcaaca atcccgacca ctgccgctag
    16561 cataagcagg agttgccagc cgcctttgcg taacatgacc agtaatgtgg ataaacccaa
    16621 tagcgccaga tacagcgcac caaagataat ttcccgactc agccatgaac tggaaaagtg
    16681 gctcagcgca tgcatcgcat taagtggata acccaggtgt aaggtagaaa tcagtaaccc
    16741 tacggcacct gccaaaaaag ccaacagtaa tggcggccgc ataaccgggc gggcgctgga
    16801 ggtgattaaa atatgacgat gaagaaacgc cagccacaac gtcataccga ccgatgcctg
    16861 taacaacagc gtaaaagcca ttaacggtaa ttcatgcatg atgtgacgcc tccttttccg
    16921 cgccctgatg cggtttaatc accagatttg gctgcgtaat cgacgaatca ggtaaacctt
    16981 tcacatcact gacatcgcca tacttttcac gcaactcatc aatagggccg aacttaatcg
    17041 cccccaacgg gcacgttgcc acgcaaatgg gttgttctcc tttagcttgc agatcaatac
    17101 agaaatcgca tttcgacatc tgcccggtct gctcattcat ttgtggtgcg ccgtaaggac
    17161 aggaccaggc gcagtaaccg caaccgacgc acttatcagt attcacgcgc acaataccgt
    17221 ctccgggacg cttatgcatc gccatcgtcg ggcaattttt ggtacagatc ggatccgcgc
    17281 aatggttaca ggaaacagaa agggtataag caaagacatt atgactcaca ccgccctctc
    17341 cagtggcgat gaagccacct ccgttcactt catacacgcg gcgaaaacgt cgcccaacct
    17401 caaggttgtt tttgtcttta caggccacct gacacgcttt acagcccgaa caacgcgaag
    17461 agtcaataaa gaaccctaat tgtttgtcgc tgacagatgc aaattcttta aattggctca
    17521 tgctttctca acctccacca gcattgtttg atgcgaattc cctttcgcca gcgccgtaat
    17581 gcgggtcgaa ctcagtacat tggcacagcc tccgcgatcg ataccttgtg catccggttg
    17641 ccaccatgcc cccgcctgca tagcaactac gccagggatg atacgggggg ttatttccac
    17701 cgggataatg ctcacaccac gatcgttata aatttttacg ctatctcctt cgctaatccc
    17761 gcgctgtttt gcatcttgcg gattcagcca gagtttttgc gtctgaactt cttgcagcca
    17821 cgggttagca tactgggtgg aattagcacg gtttttccct ttccaggtga tgagctgcaa
    17881 cgggtatttc gccgtcagtt tatcttctgg cccttcaaag gcagggacat agtgcgaaag
    17941 cgcgggaatt tctggatctt gcatatcaaa aagacgctta gagaagatct caattttgcc
    18001 ggaaggcgtc gggaaaggat tattctgcgg atcgcgaata tttgcttcga aagcgatgtg
    18061 cggcgcgctt ttgaaaagat gtcgacgtgt tttttgcagg gttgcgaagt ccggcaaatt
    18121 ttcatccggc atcgccagac gagtttgctc ccagatatgc tcgatccatt gcttctcatc
    18181 acgtccttga ctgaactcgg cttcgacacc cagttttttc gccacgtcac gcaaccagtc
    18241 ataatcggtg cgacgttcaa aatccggctc aatcagtttt tctgacagaa taagatagct
    18301 cgcagttccc catgtttcgc cgatattcca gcgttccata aagctggttt ccggcagcaa
    18361 cacatcggca tatttcgcgc tcggagtcat gaacagatcg ctgagtacga tgaattctat
    18421 tttagattcg tcttcaagga gcttcgcggc ctggtggacg tccgggttct ggttcgccag
    18481 ataattacct gccagtgaga acagcaggcg gatattactg tcgagtttat cgacaccttt
    18541 aagcccatct tgcggcgtaa ctttagaagc atcatccgcc gcctgcatcc agttcataat
    18601 cgagattttc gcttgtaccg gattttctgg catatctggt ccaacgcaga atttacgatt
    18661 agaactaccg ccgtaacctg ccgcccagcc acctttgatg ccgacgttac cggtaatcga
    18721 ggccagtaaa gttgaacctc gggcggtgcg ttcgccgcaa atatgtcgct gtggccccca
    18781 tccctgaatc aacgctgcgg gttttgtagt ggcgtaatcg cgtgccagtt gacgaatgga
    18841 ttgtgctgga acgtgggtaa ttttttctgc ccattccggc gttttgtgta tcccatcttt
    18901 tgcgccaaaa aggtaggcaa ccaacgattc gttggctggt acgccttccg gcatggagtt
    18961 ttcatcaaaa ccgagagtat atgtgtcgat aaatgtctta tcgtggagat tctcgctgat
    19021 aatgacgtac atcatcgcgt ccatcaacgc attgtccgtg gtcggcaaca gcgggatcca
    19081 ctgatcggca agtgaagata cggtgtcgga atagcgcgga tccaccacga taaaacgtgt
    19141 accgttttgc ttcatttttt gaaaatagta attggtgtga ccgaatatgg tttccgttgg
    19201 gttatgcccc cagagaatca ccagtttggt atccagaaga gtat
//
LOCUS       DAZFBR010000089        19170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFBR010000089 DAZFBR010000000
VERSION     DAZFBR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.089"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            227..3112
                     /gene="ycgV"
                     /locus_tag="ACHJZ2_003466"
     CDS             227..3112
                     /gene="ycgV"
                     /locus_tag="ACHJZ2_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4569515.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAILFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTSGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTSYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNIYVKTGAIREFSGDTEYQLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYHFSF"
     gene            complement(3212..5131)
                     /gene="dhaR"
                     /locus_tag="ACHJZ2_003467"
     CDS             complement(3212..5131)
                     /gene="dhaR"
                     /locus_tag="ACHJZ2_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO4569516.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCVTPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5359..5700
                     /locus_tag="ACHJZ2_003468"
                     /pseudo
     CDS             5359..5700
                     /locus_tag="ACHJZ2_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
     gene            5690..5836
                     /locus_tag="ACHJZ2_003469"
                     /pseudo
     CDS             5690..5836
                     /locus_tag="ACHJZ2_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
     gene            complement(5838..6449)
                     /gene="emtA"
                     /locus_tag="ACHJZ2_003470"
     CDS             complement(5838..6449)
                     /gene="emtA"
                     /locus_tag="ACHJZ2_003470"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO4569517.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            6549..7463
                     /gene="ldcA"
                     /locus_tag="ACHJZ2_003471"
     CDS             6549..7463
                     /gene="ldcA"
                     /locus_tag="ACHJZ2_003471"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO4569518.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            7559..9295
                     /gene="cvrA"
                     /locus_tag="ACHJZ2_003472"
     CDS             7559..9295
                     /gene="cvrA"
                     /locus_tag="ACHJZ2_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO4569519.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(9685..10755)
                     /gene="dadX"
                     /locus_tag="ACHJZ2_003473"
     CDS             complement(9685..10755)
                     /gene="dadX"
                     /locus_tag="ACHJZ2_003473"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO4569520.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(10765..12063)
                     /gene="dadA"
                     /locus_tag="ACHJZ2_003474"
     CDS             complement(10765..12063)
                     /gene="dadA"
                     /locus_tag="ACHJZ2_003474"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO4569521.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            12426..13958
                     /gene="ycgB"
                     /locus_tag="ACHJZ2_003475"
     CDS             12426..13958
                     /gene="ycgB"
                     /locus_tag="ACHJZ2_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO4569522.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(14010..14729)
                     /gene="fadR"
                     /locus_tag="ACHJZ2_003476"
     CDS             complement(14010..14729)
                     /gene="fadR"
                     /locus_tag="ACHJZ2_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO4569523.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            14951..16492
                     /gene="nhaB"
                     /locus_tag="ACHJZ2_003477"
     CDS             14951..16492
                     /gene="nhaB"
                     /locus_tag="ACHJZ2_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO4569524.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            16638..17168
                     /gene="dsbB"
                     /locus_tag="ACHJZ2_003478"
     CDS             16638..17168
                     /gene="dsbB"
                     /locus_tag="ACHJZ2_003478"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO4569525.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(17214..18482)
                     /gene="umuC"
                     /locus_tag="ACHJZ2_003479"
     CDS             complement(17214..18482)
                     /gene="umuC"
                     /locus_tag="ACHJZ2_003479"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO4569526.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(18482..18901)
                     /gene="umuD"
                     /locus_tag="ACHJZ2_003480"
     CDS             complement(18482..18901)
                     /gene="umuD"
                     /locus_tag="ACHJZ2_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO4569527.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         4618 a         4965 c         4772 g         4815 t
ORIGIN      
        1 acaacttagg gaataactcg atctaaactc acctttgtaa gggggatgtt aaatatatat
       61 taatttctgt gtttctgatt ttgcccggta atagagaatt attctcagag aagctcatca
      121 tgattgcacc atacaaatat catgatgata agaggatatt attcattgac ttaaacaaaa
      181 tgacaaaatc catagaccca tagcctgggt actatatagg agtggtatgg gcatcaaaca
      241 acacaatggg aataccaaag ccgatcgtct cgctgaatta aaaatccgtt cgccctcaat
      301 tcaactgata aaatttggcg ctattggttt gaatgcaatt ctcttttccc ccctgctgat
      361 agctgctgat acaggaagtc aatatggcac caatattact attaatgatg gtgacagaat
      421 tactggagat accgccgatc catcaggaaa cctctatggt gtaatgaccc cagcaggaaa
      481 cacgcctggc aatatcaacc tgggtaatga tgtcaccgtc aatgtcaacg acgcctctgg
      541 atatgcaaaa ggaatcatta ttcagggcaa aaacagctcc ctgacagcta accgactcac
      601 agtagatgtt gttggtcaaa cctctgccat cggcattaat ttaattggtg actataccca
      661 tgctgactta ggcacaggca gcaccattaa gagtaacgat gacggcatca ttattgggca
      721 tagctcaaca ctaacagcca ctcaattcac cattgaaaac tcgaacggta taggcctaac
      781 catcaatgac tatggcacca gtgtcgatct tggaagcgga agtaaaatca agaccgatgg
      841 aagtacaggt gtttatatcg gtggtctcaa cggcaataac gccaatggtg ctgcgcgttt
      901 tacggcgaca gacctgacaa tcgatgttca gggctacagc gccatgggga taaacgtaca
      961 gaaaaactct gttgtcgatc tcggaacaaa cagttccatt aaaaccagtg gcgataatgc
     1021 acacggcctc tggagctttg gccaggttag cgcgaatgca ctcactgttg atgtaactgg
     1081 agccgcggcc aatggcgtcg aagttcgtgg tggtacaacc actatcggtg cagatagcca
     1141 tatttcttcc gcgcagggcg gtggtctcgt caccagtggt tcagacgcga caatcaattt
     1201 ttctggcacg gcagcgcaac gaaacagcat cttttccggc ggttcttatg gtgcctcggc
     1261 ccagacggca acggctgtta tcaacatgca aaataccgat attacggttg atcgtaatgg
     1321 cagtctggcg ctgggtttgt gggcgctcag cggcggtaga ataaccggag acagtttggc
     1381 tatcaccggc gcggcaggag ccagggggat ttatgccatg accaacagcc agatcgacct
     1441 cacgagcgat ctggtcattg atatgagtac acccgaccag atggccatcg caacgcaaca
     1501 tgacgatggt tatgccgcca gccgcatcaa cgcctcgggt cgtatgctta tcaacggtag
     1561 cgttctttcc aaaggtgggc taatcaatct ggatatgcac cctgggtcgg tttggacagg
     1621 ttcctccctc agcgataatg tcaatggcgg gaaactggac gttgcaatga ataacagcgt
     1681 ctggaacgta acaagtaatt ctaatctcga cacgctggcg ctgagccatt caactgtcga
     1741 ttttgccagc cacgggtcaa ctgccggcac atttaccaca ttaaacgtag agaacctgag
     1801 cggtaacagt acctttatta tgcgtgctga tgttgttggc gagggtaatg gcgttaataa
     1861 taaaggggat ttattgaata tcagcgggag tagtgctggt aatcacgtat tggctatccg
     1921 caaccagggc agcgaggcca caacgggaaa tgaagttctg acagtggtaa aaaccactga
     1981 cggtgcggcc tcgttcagcg cgtcttctca ggttgagttg gggggatatc tgtacgatgt
     2041 gcgtaaaaat ggcactaact gggagcttta cgcttccggg acagttccgg aaccgactcc
     2101 taatcctgaa ccaacaccag ctcccgctca gcctcccata gtcaaccccg atcctacgcc
     2161 tgaacccgat cctacgccta accccactcc cacacctaaa cccaccacga ccgcagatgc
     2221 tggcggcaat tatctcaatg tcggttactt attaaactat gttgaaaacc gtacgctgat
     2281 gcaacgaatg ggtgacctgc gaaatcagag taaagacggt aatatctggc tgcgcagtta
     2341 tgggggaagc ctggactcct tcgccagtgg caaactgagc ggctttgaca tgggttacag
     2401 cggtatccag tttggtgggg ataaacgtct ctctgatgta atgccgttgt atgtcggtct
     2461 gtatattggc tcaacacatg catcgccgga ctatagcggt ggcgacggta ccgcacgttc
     2521 agactacatg ggaatgtacg ccagttacat ggcacacaac ggtttttaca gcgatctcgt
     2581 tgtaaaagca tcgcgccaga aaaatagttt ccacgtactg gacagtcaga acaacggcgt
     2641 taacgccaac ggcactgcga atggattgag catctccctg gaagccgggc agaggttcaa
     2701 cctgacccct actagttatg ggttctatat agagccgcaa acccagctca catacagcca
     2761 ccagaatgag atggctatga aggcgagtaa tggtctcaat atacatctga atcactacga
     2821 atcgctgctg gggcgtgcca gcatgatact gggatatgac atcaccgcag gcaacagcca
     2881 gctgaatatc tatgtgaaga ctggcgctat ccgcgagttt tcaggggata cagaatatca
     2941 gttgaataac tcccgggaga agtacagttt caaaggtaat ggctggaata acggcgtggg
     3001 agtcagtgca cagtataaca aacagcacac attctacctc gaagcggatt acacgcaggg
     3061 taacctcttt gatcagaagc aagtcaacgg aggatatcac ttcagctttt aagtacgggg
     3121 tagacaagcc cttaaacgat gctgaaaaat gttagcgtgc gccggaatga aatatagatc
     3181 actctgatgt atcagaaatc gtctctgtct ttcatacccg gcgcttaaac tgccctgcat
     3241 caatgccatg ttgcttcatt ttccgccaca gcgtagtgcg gccgattcca agtaaagccg
     3301 acatttcctg aatgcgaccg cctgtgacct gggctgcgtt aataattgcc tctttttcaa
     3361 cttccgcaaa tgacagactg gtggaaaggc gtgtggcgct gacatcatct gtcgcctgct
     3421 cggtaaacag atgttccggc aaatcactga cgcgaatgcg cccattatca ctactcagag
     3481 caagattctc gatgacgctg taaagttcaa aatcgttgcc aggccatgca caggaaacca
     3541 ggcgagcgag ggcatcgtca tcaattttca gccgcgtaga gaagcgtttt tcaagactgc
     3601 gtaatttgtt attcaccaga gccggaatgc tgccacgccg catacgcaga ggcgggatgg
     3661 taatttcaaa tgcatgcagc gcgtaataca gctggcgact aaaacgattt tgttccacca
     3721 gcattgcgag gtccgcggtc gttgtagcaa tcactttgac atcaattggt attaaacgcc
     3781 gcgcatccag tcgcgtgata acaccctgct tgataacctg aagcaaagca gactgtaact
     3841 ccaccgccag atattcaatc ttttcaagaa acagcgtgcc gccgtgcgcc agttccagcc
     3901 gactcagacg gccattttca ttgtccgtgc gatcgccacc aataaattct tccgccagtg
     3961 ccgcatcacc atataactca caattgacgg cgatataagg acctgcagca cgctcgcttt
     4021 cattatgaat tgcctgactt agcagtgcct tgcccacgcc ctcttctcca caaagcagga
     4081 caggaaagct actgcgcgcc gcctggcgac caaaatgaat caagcggcgg gtttgcggat
     4141 cgtcctgtgg catatgagcg aaggtatggc tgacttttcc taattgactg gtcattaact
     4201 gccgcatctg ttccacagga tggagcaaca aaataaagct ggttccctgc gtttctatta
     4261 tcggtttaag ggttatcacc gcatcaataa actggtgctg gctttcaaag gttgcttcta
     4321 cgtgtttgag cggatgtgcc tgttttattg cttgttgcaa tacggcgggt aacgttaaga
     4381 gttcagtgat tgcccgtccc tgacttgccg tcgcgtcaag gcgcaagacc cgcgccgcct
     4441 gggcattaat aaattgcaaa ttaccctgct cgtcccagct aatcacgcca tcatccatac
     4501 tttctaacag ggcattaagt tgatttaaat gacggttagt ttcagcgagc aaactgtccg
     4561 tcagcagtaa atttccgacc tcgcgggcga ttgccaacgt caacggcaaa tcagctgcgg
     4621 tagtttgctc aaccggacac gccagcgcta ttgttcccgt caatcggccc ttgctgtcaa
     4681 ataacggcgt tacacaaaag gcccagttcc agagtgcctg tttgaaatgt tgatcggcca
     4741 tcgttttcac ggcctgacca gagatagccg ctaacgatag cgcacaagta ccaataattc
     4801 cctcggcgca atacgtgccg tcattgaatc ccagtgcact tagctgctgc aaggtttgcg
     4861 gatcgccatt acggctgaga atgcaggcgg tttcatcgag gataaacagc gcacactctc
     4921 gcggtgccat atattcccag gcatcttcca gcgcagcctg cccgagcgtc agcatcgctt
     4981 tcttacgccg ataaatagaa gcaaatgtca cgccctgggc ctgatgtggt acgttccatg
     5041 tctcccgttt catcagctta ttgcatcgct cccaggaggt tgcaattaag ggagatatgc
     5101 cccgaccatc gttgttaaaa gcgccactca tatcccgcat cccttatgac aaaaggtgtt
     5161 ccggctcgca attttaagta ttaatacacg taaaagtttc aatatggaac attcattaaa
     5221 gcatatgttt cactatgaaa cataatttgg gctaattttc ttttcccttg cctgatgcac
     5281 aatggattca tcggcagtca gtggtcgccg tgtcgttgaa catcatccat gccctaccgt
     5341 aattgctgga gcaaaataat gaaaaaattg atcaatgatg tgcaagacgt actggacgaa
     5401 caactggcag gactggcgaa agcgcatcca tcgctgacac tgcatcagga tccggtgtat
     5461 gtcacccgag ctgatgcccc tgttgcagga aaagtcgccc tgctgtcggg tggcggcagc
     5521 ggacacgagc cgatgcactg tggttatatc ggtcagggga tgctttcggg ggcctgtccg
     5581 ggcgaaattt tcacctcacc gacgcccgat aaaatctttg aatgcgccat gcaagttgat
     5641 ggcggcgaag gtgtactgtt gattatcaaa aattacaccg gcgatattct tagatggcaa
     5701 gaatgaaacc atcaccactc cccgtctgag cttccgtttt cttaacgtca gcccgacggt
     5761 ggagcggcaa ttacagcgga ttattttctc tctcgagcgg gaagcccggg agaaagcgga
     5821 caaagtacgc gactgattta catcgcgtcc agtgcctgct caagtttgta gatatagcgc
     5881 ggagcctgcg gcgcaggatg atttcgcgct acgtgatcga ggaactcgtc agcatccaga
     5941 tcgttgattt tgctgattgc ctttttccga tctgacgaga aagtccgtag cagcgcacct
     6001 gccccgttag catatgacac caccagcgca tattgcagta ccttcggatc ttcaatgcct
     6061 gccagcgggc cggtttccag aatattcagg taagccgccc ccatagaaat attacgctcc
     6121 ggatttttca gctcgctggt cgtcggctca ccactccagc ccatacgccg ataaacatca
     6181 cgtccggagg ttgaagcttt taactgcatc aaaccaatgg cattcgattt actcaccgcg
     6241 ttcggattac cacccgattc gatagcgata atcgccgtga tcaattgtgg atcgacgccc
     6301 caggctgcac cggctttctg gcttattggc atccactgca tcgcacgttg caccggaact
     6361 ttcgcgttcc acggcgggtt cgtatagtca tgctttgatg aacaacccgc taataacaca
     6421 atcaaaaagg caaaccatct caatttcact tcttctatcc ttatagcctg gaatcgccgt
     6481 tgaggtgaca acggcacacc taagcggcat gatatacatt tcgtatctgt tgtcagcaag
     6541 gaattgccat gtctctgttt cacttaattg ccccatcggg ttactgcatt aaacagcacg
     6601 ccgcgctgcg tggtatccag cgccttaccg acgcggggca tcaagtcaat aacgtcgagg
     6661 ttattgcccg tcgttgtgag cgttttgcgg gtacggaaac ggagcgtctg gaagatctca
     6721 attcccttgc cagactgact acccccaaca ccatcgtgct ggctgtacgc ggcggttacg
     6781 gtgccagtcg tttactggca gatattgact ggcaggcact ggtggctcgc caacaatatg
     6841 acccgttgct catatgcggg catagcgatt ttaccgccat tcagtgcggt cttctggcgc
     6901 aaggcaatgt catcaccttt agcggcccca tgctggtagc aaactttggc gcggatgaac
     6961 tgaacacctt taccgagcac catttctggc tggcgttacg caatgaaacc tttaccattg
     7021 aatggcaagg ggaaggcccg acatgtcggg ccgaaggcac gttgtgggga ggcaatcttg
     7081 cgatgctgat ttcactgatc ggtacaccgt ggatgccaaa aattgagaac ggtattctgg
     7141 tgctggaaga tattaacgag caccctttcc gcgtcgaacg tatgctgttg cagctctatc
     7201 atgcgggaat tttgccacgt cagaaggcga ttattctcgg tagctttagc ggcagtacgc
     7261 ctaatgatta tgacgcaggt tacaacctgg agtcagtgta cgcatttttg cgttcccgcc
     7321 tgtcgattcc actcattacc ggcctcgatt ttggtcatga acaacgcacg gttacgctgc
     7381 cgttgggcgc acatgccatt cttactaaca cccgggaagg cactcagttg acgattagcg
     7441 gtcatcctgt tcttaaaatg taaaaaaaat cacgcttcag ggctaagtaa aacgctgtct
     7501 ctgccgctaa tatggtaggg tttatataag agacagcgta atcaggagta accgaccgtt
     7561 ggatgccaca acaataatta gccttttcat cttaggatcc atccttgtta ccagcagtat
     7621 attacttagt tcattttctt ctcgtcttgg cattcctatt ctggttattt ttctggcgat
     7681 cggcatgctg gcgggagtcg atggcgtcgg cggtatccca tttgataatt accccttcgc
     7741 ctacatggta agtaacctgg cgctggcgat tattttgctc gatggcggga tgcgcactca
     7801 ggccagttcc tttcgtgtgg cgttaggacc ggcgctgtcg ctggcgacgc tgggcgtact
     7861 tatcacctct ggtttaaccg gcatgatggc ggcgtggctg tttaatcttg atttgattga
     7921 aggcttatta atcggcgcta tcgtcggctc caccgatgct gcagcggtct tttctctgct
     7981 gggtggtaag gggcttaacg aacgtgttgg ctcgacgctg gaaattgaat ccggcagtaa
     8041 tgatccaatg gcggtctttc tgacgattac cctgattgcg atgatccagc agcatgaaag
     8101 cagtgtcagt tggatgttcg tggtcgatat tctgcagcaa tttggcctcg ggattgtcat
     8161 cggtcttggc ggtggttatt tactgctgca aatgattaat cgcatcgccc tgcccgccgg
     8221 tttatatcca ttgctggcat taagcggcgg tattttaatt tttgctttaa ccacggcgct
     8281 ggaaggtagc ggtattctgg ctgtttatct gtgcggtttt ctgctgggta atcggccgat
     8341 tcgcaaccgc tacggcatcc tgcaaaattt cgacggcctc gcctggctgg cgcaaatcgc
     8401 catgttcctg gtgctggggc tgttggttaa cccaagcgat ctgctgccta ttgccattcc
     8461 ggcgctcatt ttgtccgcat ggatgatttt cttcgcccgt cctctttcgg tatttgccgg
     8521 attgctacca ttccgtggct tcaatctgcg tgagcgcgtg tttatcagct gggtgggatt
     8581 acgcggcgcg gtgccgatta ttctggccgt gttcccgatg atggcggggc tggagaatgc
     8641 acgtctgttc tttaatgtcg ccttctttgt ggttctggtt tcactgctct tgcagggaac
     8701 atcactctcg tgggcggcga aaaaagccaa agtggtggtt ccgcccgtcg gacgtccggt
     8761 gtcacgcgtt ggcctggata ttcatccgga aaatccgtgg gagcagtttg tttatcaatt
     8821 gagtgccgat aaatggtgcg tgggcgcggc actgcgcgat ttgcatatgc caaaagagac
     8881 gcgtattgcg gcactgtttc gtgataacca gttgcttcat cctaccggca gcacccgact
     8941 gcgcgaaggc gatgtgttgt gtgtaattgg tcgggaacgc gatctcccgg cgctcggtaa
     9001 actgttcagc cagtcgccgc cggtcgcgct ggatcaacgc ttctttggtg acttcattct
     9061 cgaagccagc gccaaatatg cagatgtggc gctgatatat ggcctggaag acgggcgaga
     9121 atatcgtgat aagcaacaaa cgctgggtga aattgtccag cagttgttag gcgcggcacc
     9181 ggttgtcggt gaccaggtag agtttgccgg gatgatctgg acggtggccg agaaagaaga
     9241 taatgaagtg ttgaagattg gcgttcgagt ggctgaagag gaagctgaat cttaaattta
     9301 atgaagcatc tatcgccggt tacgatgctt tgcttaacgc agcctgagcc tgtatataaa
     9361 cttgtcattt cctgattttg atatgcacat tccaacatca gaagaaggcc tgttaatcaa
     9421 cattctgaac atgaacccga atctgcatca agggtgaagc gtgatttatc tgaagtcgag
     9481 ttcgagtcaa caatgaagcg taagcgtttt atgtagaacg cctgtgcggt gagttcaggt
     9541 tccggggaaa tagtgtccat ccttttaatg ggcactatca tgggcaatgg ctctgtcaca
     9601 ttaacaacta cagttgctga ccagccggat ggatgatttt ttttgcaacc agaagacgtt
     9661 gcctccgatc cgacttacaa caagttacac cgtcacaacc gggacgcgta acgccagcgc
     9721 gcacatcaac tcatagccca ccgttccggc agcggcggcg acatcatcaa ttttgatctc
     9781 cttgccccac agctcaactg gcgtaccaat acccgcctgc gggcaaggcg ttaaatcgac
     9841 cgccagcata tccatcgaga ctgtccccac cgtcatggtg cgcacgccgt ccactaaaac
     9901 aggggtaccg gtaggcgcgt ggcgcggata accgtcggcg tatcctgcgg caacaatgcc
     9961 gattcgctgc tcatcgcgcg cagtataacg accgccgtag cccacacgct cgcccgcttt
    10021 tagcgtctgg acaccaataa tctcactgct tagcgtcatc accggacgta atccagtatt
    10081 ggcgatatca cgccactgac cggacggcga agcgccatac aaaataatgc caggccgaac
    10141 ccagtcaaaa tgcgcttctg ggtgccacag agtcgccgcc gaattggaca acgaacgccg
    10201 acactccagc ccttccgccg cctgctcaat acgcgccatc gcgccggaaa ttccatcagg
    10261 atgttccgcc tcggcaaaat gcgacatcag ggtcatttcg ccaacattcg ccatcgcccg
    10321 caactgctgc cagacggtaa gcacgcgatc gggctggaag cccaaccgat tcatcccact
    10381 gttcacttta agataaatat ccaacggtgc ttttagccgc gcattttgca gtgctttgag
    10441 ctgccagttg ctgtgtacgc aggtggtcag gcggtgctgg tcatacattt ccagatcctg
    10501 agcatggaaa aatccttcca gcatcaggat cggtcctttc cagccgcgct cacgtaacgt
    10561 tattgcttct tccaggttaa gtaatgcaaa gccatcggta gccccgagcg cgctccagat
    10621 acgctcaata ccgtgcccgt aagcgttcgc ttttaccacc gaccagacgc gcgcgtgcgg
    10681 cgcggcctgg cggacaatgg acagattctg ttttaatgcc tgcagatcga ggctggcctg
    10741 tatcggacgg gtcatctcgt ttccttagct gtgtgcgcca tgtaaatggc ccggacgtga
    10801 tggcgtaaat ccacggctgt agcgcgctac gcttagatcc tcatatggga tcgctggcgt
    10861 gcgaccagag agcagatcgc ttaacaactg accggaaccg caagccatcg tccagccgag
    10921 cgtgccgtga ccggtattta gccacagatt tttaaagcgt gtacgcccga caaccggcgt
    10981 gccgtctggc gtcatcgggc gcagacccgt ccagaaagtc gcctgctcga catgaccgcc
    11041 gcgtggatag agatcgcgaa ccaccatctc cagcgtttca cgacgcggtt gcaacagctc
    11101 ggtattaaaa ccaacaattt ccgccattcc gccaacacga atgcggttat cgaaacgggt
    11161 aatggcgatt ttgtaggttt catcaagaat ggtggatacc ggcgcaccat cttcctgcgc
    11221 aattggaatg gtcagcgagt agcctttcag cggatacacc ggaatatcaa caatgccttt
    11281 gagcatcgcc gtcgagtaag aaccaaacgc catcacatac gcatcggcct taatcacttc
    11341 atcgccacac ttcacgccgt agatttgctc gccgtcgcaa agcagttggt caacgggcgt
    11401 attaaagcgg aatttaaccc ccgcctgctc cgccatccgc gccagattct gggtaaacag
    11461 ctggcagtct ccggtttcat cattgggcaa ttgcagacca cccgtcagtt tgtgcgctac
    11521 ttctgccagc gcgggctcca cttccgccag gcggctggat tccagcagct gatacggtac
    11581 gccggcatct tccagcacgg cgatatcgcg ggtcgcattt tcatactgtt gttcggtacg
    11641 gaacagttgc agcgtcccac cctgacgccc ttcatactga atattggttt cggcgcgcaa
    11701 tgctttcagg caatcacggc tgtattccgc cagacgcacc atccgccctt tgttttccat
    11761 gtagtggctg gtgtcgcagt tacgtaacat ttgccacatc catttcaact ggaactgcgt
    11821 accgtcgaga cgaaccgcca gcggcgcatg gcgctggaac atccatttaa tcgcttttaa
    11881 aggcacacct ggtgccgccc acggtgcagc atatccgggg gagatttgcc cggcattcgc
    11941 ggcactggtt tccagggcgg cccccggctc ccgatcaatg acggtgacct catgtcctgc
    12001 ctgatttaag taccaggcgc tggcaacgcc taccacacca cttcccagta tgacaactcg
    12061 catagccact ccgataacgg tagatacagt aaaagaataa taatctaatt acatcttgat
    12121 aactcagttg aaaatattat tcaacatacg gcttttttat ggtgagctgg ctcacatctc
    12181 cctgactctg cggcaatatg gcgtctttag tatctcctgc tgaacgccct ttttatccag
    12241 cataaaattc cgttcagaaa cggattagtg gcactctgat aataagaaat cgcaaagaaa
    12301 aaaattctcc ggcgacgcgg tttttaacag gtgttctatg cttgaaatga ggtgctccac
    12361 acagagagcg ccgacaacaa tgaggtgctc cacacagaga gcgccgacaa caatgaggtg
    12421 cgcgtatggc gacgatcgat tctatgaata aggacaccac acgtttgagc gatggacccg
    12481 actggacgtt cgacctgctg gatgtttatc tggcagagat agaccgggtg gcgaaactct
    12541 accggctgga tacctacccg caccagattg aagtgataac ctcagaacag atgatggatg
    12601 cctactccag cgtcggcatg ccaattaact atccgcactg gtcattcggt aaaaagttta
    12661 tcgagactga acggctgtat aagcacggtc agcaaggact ggcctatgaa atcgtcatta
    12721 actctaaccc gtgtatcgct tacctgatgg aagagaacac cattaccatg caagcgctgg
    12781 tgatggctca tgcctgctat gggcataact ctttcttcaa aaacaattac ttattccgta
    12841 gctggaccga cgccagttcg attgtcgatt atctgatttt tgcccgtaaa tatattaccg
    12901 agtgcgaaga acgttatggc gttgatgaag tagaacggct tctggactcg tgccacgcgc
    12961 tgatgaacta cggcgtggac cggtacaaac gcccgcaaaa aatctcgctg caagaagaga
    13021 aagcccggca gaaaagtcgc gaagagtatt tgcaaagtca ggtcaatatg ctctggcgta
    13081 ccctgccgaa gcgcgaggaa gagaaaacgg ttgctgaagc gcgccgttat ccgtccgaac
    13141 cacaagaaaa cctgctctat tttatggaga aaaatgcgcc actgctggaa tcatggcagc
    13201 gtgaaatcct gcgtattgtg cgtaaggtga gccagtattt ttatccgcaa aaacagactc
    13261 aggtgatgaa cgaaggctgg gccaccttct ggcactacac catccttaac catctgtatg
    13321 atgaagggaa agtaacggaa cgttttatgc tggagttttt gcacagccac accaatgtgg
    13381 tcttccagcc cccctataac agcccgtggt acagcggcat caacccgtat gccctcgggt
    13441 tcgccatgtt ccaggatatt aaacggattt gtcagtcgcc aacggaagaa gacaaatact
    13501 ggttcccgga tatcgccggt tccgactggc tggaaacgct gcatttcgcg atgcgtgatt
    13561 tcaaagatga gagttttatc agccagttcc tgtcgccgaa agtgatgcgt gatttccgct
    13621 tcttcaccgt gctggatgat gatcggcata attatctgga gatttccgct attcataatg
    13681 aagaaggtta tcgggagatc cgtaaccggc tatcgtcgca atataactta agtaatctgg
    13741 agccgaatat tcagatctgg aacgtggatt tgcgcggcga ccgttcgctg acgctgcgtt
    13801 acattccaca taatcgcgca ccgctggatc gggggcgcaa agaagtactg aagcatgtgc
    13861 atcgcctgtg gggatttgat gtgatgctgg aacagcaaaa cgaagacggc agcgtcgagt
    13921 tgctggaacg ttgcccgccg agaatgggaa atctgtaaaa acaacaaaaa acccctcgtt
    13981 tgaggggttt gttttttaaa cggaagggat tatcgcccct gaatggctaa atcacccggc
    14041 agatttttct gcatccggtg ccaaatctcg ccactctcat gcccatagcg acgcactgtt
    14101 tcgtacacct gatcgtgcgc gccttcactg cacaacgccg acagtttgtg gtagaagccc
    14161 agcgccagac tgcgcgcttc cggattggcg aaatagtgac gaccaatgcg cgtatacagt
    14221 cccttcatcc cgttaaggat cagaccgtaa atcgggttgc cggaagcaaa cgccaggccg
    14281 cggaatatgt tgtaatccag ctcggcaaag gcatcggcgt gatcggccac ttcattagcg
    14341 gtagccagca cttcctgcgc tttatcggga tgctgacgaa acgcggtgcg aataaaaata
    14401 gttgaaatat tggtacgcac cgacagcaaa ttatcaataa gctgcggcac actttcgtga
    14461 tccagtcgcg ccagtgtttc aaggatattt aaaccagagg tttcccagaa attattcacc
    14521 ttcgtcggct tgccatgttg aatggtcaac cagccatctc gtgccagacg ctgtaacact
    14581 tcacgtaacg tagtacgcgt tacgccaatt aattctgaaa gttcacgttc tgcgggcaaa
    14641 atagtcccgg gagggaagcg gttattccag atactttcaa taatgtactc ttccgcgaaa
    14701 cccgccgggc tttgcgcctt aatgaccata gtgagatttc cataacacag caaaacaaag
    14761 ttggactcat cataccagag ggctaactac gctgatagca gacaaaaaga agaaaaaagg
    14821 gatcaaggct tcattttccg catctctttc cccttgacta tcaagacttg cctcactcct
    14881 gcctgaccgt taagctttgc gtttaaacat aaaaacatca ttgattattt gtaaggtaag
    14941 ggaaaccatc atggagatct cctggggccg cgcgctatgg cgcaattttt tgggccagtc
    15001 ccccgactgg tacaaactcg ccctcatcat tttcttaatc gtaaatccgt taattttcct
    15061 catcagccct ttcgtcgctg gctggttgct ggtagcggaa tttattttca ctctggcgat
    15121 ggccctgaaa tgctacccgc tgctccccgg tggcctgttg gctatcgaag cggtattcat
    15181 cggcatgacc agcgcggaac acgtccgtga agaggtggcg gcaaatcttg aagtcttgct
    15241 gttactgatg tttatggtgg cgggtatcta ttttatgaaa cagctattgc tgttcatatt
    15301 tacccgtttg ctgttaagca ttcgctccaa aatgctgctg tcgctctctt tttgcgtagc
    15361 ggcagcgttc ctctccgcgt tcctcgatgc cttaaccgtc gtggcggtgg tgatcagcgt
    15421 tgccgtcggt ttttatggta tttatcatcg cgtagcctct tcccgtaccg aagacaccga
    15481 cctgcaagac gatagtcata tcgacaagca ttacaaagtg gttctggaac agttccgtgg
    15541 ctttctgcgt agcctgatga tgcatgccgg tgtcggcacc gcattaggcg gcgtaatgac
    15601 catggtgggc gaaccacaga acctgatcat cgctaaagcg gctggctggc attttggcga
    15661 tttcttcctg cgcatgtcgc cggtgaccgt tccggttctg atttgtggcc tgttaacctg
    15721 cctgctggta gagaagctgc gttggtttgg ctacggtgaa acgctgccgg agaaagtccg
    15781 cgaagtgttg caacagtttg acgatcaaag ccgccaccag cgtacccgtc aggataaaat
    15841 ccgtctgatt gtccaggcga ttattggcgt ctggctggtg actgcgctgg cgttgcatct
    15901 ggcggaagtt ggcttgattg gtttgtcagt cattattctg gcaacatcat tgaccggtgt
    15961 caccgatgaa catgctatcg gtaaagcctt caccgaatct ctgccattca ccgcactgtt
    16021 gacggtgttt ttctcggtag tcgcggtgat tatcgaccaa caactgtttt cgccgattat
    16081 tcagtttgtg ttgcaggcat cggaacatgc tcagctgtcg ctgttctata ttttcaacgg
    16141 tctgctgtca tccatttcgg ataacgtctt cgtggggacg atttatatca acgaagcgaa
    16201 agcggcaatg gaaagtggcg ctatcacgtt gaagcaatac gagctgctgg cggtcgccat
    16261 taataccggt accaatctgc cctctgtcgc tacgccgaac ggtcaggctg cgttcctgtt
    16321 cctgctgacc tctgcactcg cgccattgat tcgcctctct tatggccgca tggtgtggat
    16381 ggccctgcct tacaccctcg tcctgacact cgtcggactg ctctgcgtcg agtttacgct
    16441 tgcccctgta accgaatggt ttatgcaaat gggctggata gcaacgcttt gataacaact
    16501 taccgggcat ataaatgccc ggtttgcctt ttcgcccgat aattgtccaa ttgcgtccta
    16561 tttttgctgc ctcctggtgg cgcagcgaat gaattggttt aaactgcgca ctctatgcat
    16621 attgcaggga aatgattatg ttacgatttt tgaaccaatg ttcacaaggc cggggcgcgt
    16681 ggctgttgat ggcgtttact gctctggcac tggaactgac ggcgctgtgg ttccagcatg
    16741 tgatgttact gaaaccttgc gtgctctgta tttatgaacg ctgcgcatta ttcggcgttc
    16801 tgggtgctgc gctgattggc gcgatcgccc caaaaacgcc actgcgttat gtagcgatgg
    16861 ttatctggtt gtatagtgcg ttccgcggtg tgcagttaac ttacgagcac accatgcttc
    16921 agctctatcc ttcgccgttt gccacctgtg attttatggt tcgtttcccg gaatggctgc
    16981 cgctggataa atgggtgccg caagtgtttg tcgcctctgg cgattgcgcc gagcgtcagt
    17041 gggatttttt aggtctggaa atgccgcagt ggctgctcgg tatttttatc gcttacctga
    17101 ttgtcgcggt gctggtggtg atttcccagc cgtttaaagc gaaaaaacgt gacctgttcg
    17161 gtcgctaatc cattcggcgc tcctgcggga gcgctttttt cctgccgctg tgtttatttg
    17221 acccgcagta aatcagaact tcgcgttgta taacgtggtg aaagcatggc tcgcttcatc
    17281 tgccattgtt gctggatccc ctgcccggca aaatagagtg ttcctctgcc ctctttagca
    17341 ttcagtgtat ccattaccgc catcaattgc tcactcccgg ggcgcggagc gttgtcatcg
    17401 aataaattga gctgcgcgac tccctgactg aagaaatccc ccagcatcac gcccgctttt
    17461 tggtagcgat ggcccgcttg ccagatggca tccagagatc gcgtagcggc gttaatgata
    17521 tccctgctgt cctgagtggg cgtcagcagt tttaccgacg cgctattgcc gtaataaggt
    17581 tcattgagcg caaatggtga cgtcttaata aatgtggaga taaaccgaca atattgatgc
    17641 tcactgcgaa gtttttccgc cgcccgggca gcgtaactac aaatggcctg ccgcatcgac
    17701 gtataatccg tgatgcgttc accaaacgag cgggaacaga taatttcctg ctttgtcggt
    17761 gcaaattctt ccagttgcaa acagggttca ccgcgcagtt cacgcaccgt tctttcgagc
    17821 acgacattaa aatgtttacg gataaaccgg atatctgtat ccgccaaatc gagaacggtt
    17881 ttgatcccca tcgcgtccag ttttttgctg atccgccgtc caatccccca gacgtcatcc
    17941 acggggagag cagacattaa tttacgctgg cgttccagat ttgataaatc caccacccca
    18001 cccgtctgcc gctgccattt ttttgccgca tgattggcaa gcttagccag cgttttggtc
    18061 tgggcgatcc ccacaccaac agtaagatgg gtacgttgta gcaccgttgc gcgaatttct
    18121 ctgccaaagt cagtcagatc gcgacaatta cgcacacctg tcagatcgca gaatgcctca
    18181 tcaatactgt aaatctcgac gcggggcgat agttcttcca gcgtcgacat cacccgattg
    18241 ctcatgtctg cgtaaagctc ataattgctg ctaaagcaaa ccacgccaca gcgacgaaac
    18301 agatcttttt gtttgaacca gggatcgccc attttaacgc caagcgcctt tgcctcagcg
    18361 tttcgggcga taacgcaacc gtcattattc gatagcacaa ccaccggttt accccataac
    18421 tcagggcgaa acaccgtctc acagctggca taaaacgcgt ttacatcaca gagggcaaac
    18481 atcagcgcat cgccttaacg acgtggatca ccacaccaaa gacatccagc gtatcttcac
    18541 tactgatggt aatgggcgag tacgcgctgt tcatgggaat aagctgtacc gtcgggcgta
    18601 gttgcaattt tttcaccgta aactcgccgt caacagcagc gatgacaata tcaccatggc
    18661 tggcggtaat agcgctatcg acaatcagta aatcaccgtc actaattcca ccatcaatca
    18721 tagaatcacc acttgctttg acgaagtaag tcgcgctggg atgctggatc aacagttgat
    18781 tcagatcgat gcgctgttca acgtaatctg cggccggtga aggaaagcca cactgaacaa
    18841 gatcgctaaa tagcggaaaa gtcacaattt cgcggagatc cgcaggcttg ataaacaaca
    18901 taataatctg cctgaagtta tactgttttt atatacagta gtctgttctt gccagcagat
    18961 caatactgat tcaggctatc aatatttgtc gctgcataga ctgctgattt ttcgttctct
    19021 tatcctgtgc tcacgcggcc ttctggcgac gacgctcatc cagcagaaat gaaaaatatc
    19081 acccggctaa aaaatagaat agaagcattc gccataatga cattaaacat tgtttgatat
    19141 ttatcatatt aatagaaata aagacattga
//
LOCUS       DAZFBR010000090        19142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFBR010000090 DAZFBR010000000
VERSION     DAZFBR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.090"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(8..1462)
                     /gene="xylB"
                     /locus_tag="ACHJZ2_003481"
     CDS             complement(8..1462)
                     /gene="xylB"
                     /locus_tag="ACHJZ2_003481"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO4569528.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1534..2856)
                     /gene="xylA"
                     /locus_tag="ACHJZ2_003482"
     CDS             complement(1534..2856)
                     /gene="xylA"
                     /locus_tag="ACHJZ2_003482"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO4569529.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3222..4214
                     /gene="xylF"
                     /locus_tag="ACHJZ2_003483"
     CDS             3222..4214
                     /gene="xylF"
                     /locus_tag="ACHJZ2_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4569530.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            4292..5833
                     /gene="xylG"
                     /locus_tag="ACHJZ2_003484"
     CDS             4292..5833
                     /gene="xylG"
                     /locus_tag="ACHJZ2_003484"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO4569531.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHKGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5811..6992
                     /gene="xylH"
                     /locus_tag="ACHJZ2_003485"
     CDS             5811..6992
                     /gene="xylH"
                     /locus_tag="ACHJZ2_003485"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO4569532.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7070..8248
                     /gene="xylR"
                     /locus_tag="ACHJZ2_003486"
     CDS             7070..8248
                     /gene="xylR"
                     /locus_tag="ACHJZ2_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO4569533.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8356..9180)
                     /gene="bax"
                     /locus_tag="ACHJZ2_003487"
     CDS             complement(8356..9180)
                     /gene="bax"
                     /locus_tag="ACHJZ2_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO4569534.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9500..11530
                     /gene="malS"
                     /locus_tag="ACHJZ2_003488"
     CDS             9500..11530
                     /gene="malS"
                     /locus_tag="ACHJZ2_003488"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO4569535.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11708..12961
                     /gene="avtA"
                     /locus_tag="ACHJZ2_003489"
     CDS             11708..12961
                     /gene="avtA"
                     /locus_tag="ACHJZ2_003489"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO4569536.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13113..13586)
                     /gene="ysaA"
                     /locus_tag="ACHJZ2_003490"
     CDS             complement(13113..13586)
                     /gene="ysaA"
                     /locus_tag="ACHJZ2_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO4569537.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13688..13828)
                     /locus_tag="ACHJZ2_003491"
                     /pseudo
     CDS             complement(13688..13828)
                     /locus_tag="ACHJZ2_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            complement(13830..14645)
                     /locus_tag="ACHJZ2_003492"
     CDS             complement(13830..14645)
                     /locus_tag="ACHJZ2_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4569538.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            14871..16271
                     /locus_tag="ACHJZ2_003493"
     CDS             14871..16271
                     /locus_tag="ACHJZ2_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4569539.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQFWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            16282..18252
                     /locus_tag="ACHJZ2_003494"
     CDS             16282..18252
                     /locus_tag="ACHJZ2_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO4569540.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(18382..19122)
                     /gene="yiaT"
                     /locus_tag="ACHJZ2_003495"
     CDS             complement(18382..19122)
                     /gene="yiaT"
                     /locus_tag="ACHJZ2_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO4569541.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT         4765 a         4970 c         4733 g         4674 t
ORIGIN      
        1 ataacgttta cgccattaat ggcagaagtt gctgatagag gcgacggaat gtttcacgtc
       61 gtggctgata agcggcataa cgctgcgcat ctggtagatg cgactgttct aacggtagtt
      121 gcggcaacaa ctcaatgagc gatttctctg gattcgccgc aatctgcgcc agccttgctg
      181 cgcccagtgc tggccccaca tccccccccg tacggtaatc gagctgctga ccgctgatat
      241 ccgccagcat ctgacgccag tactcactac gcgccccgcc accaatcaac gtaacacttt
      301 gcggtttaat gccgcaggca tgcacgacat ccatgccatc tgccagcgca taacccacgc
      361 cttccagcac tgctcgcgcc agttcattgg ggccatgttg atgagtcaaa ccaaagaaaa
      421 cccctttcgc ctggggatta ttgtgtggtg tacgctcgcc ggaaagataa ggcagaaacc
      481 aaactggctc ggcactttca tcagcctgtt gagctgcagc gattaaagct gggacattgc
      541 tcaggccggt taatttcgcg gcccaatcca gacacgacgc tgcactcagc attacagaca
      601 ttaaatgcca acgctgcggt agcgcatggc aaaagctgtg tacggcgctt tctggcttgc
      661 ttaagaaccc ttcgctgaca gcaaaataga cccccgacgt ccccagcgat aacattgcct
      721 gattagcatc aaccattccc acaccaactg caccagctgc attgtcgcca ccgcctgcga
      781 caactggcac cgtcgccata ccccacgctt tcgcaacttc aggtaacaaa gcaccagtaa
      841 tttcgctgcc ttcgtataat gcgggcatct ggtcacgaga taagtcgcaa gcctgcagca
      901 tgacgtcact ccagtcacgc tttgcgacat ccagccacat ggtgccagct gcgtcagaca
      961 tatcgctggc aaactccccc gtcatacgca gacgcaagta atctttcggt aataatactt
     1021 tgtcgatttg acggaatatc tccggctcat gccgctgaac ccatagcaat ttaggcgcag
     1081 taaatccggg catcatcagg ttgccggtaa tcacccgcga tagcggaact cgcgcttcca
     1141 gcaaagcgca ctcttgcgca cagcgcccgt cgttccacaa aatggcaggg cgcaataccc
     1201 gttgttgagc atccagtaag gttgctccgt gcatctggcc ggcaataccc aatgctttaa
     1261 cgtcctgcag agaatgctga tcgcccagag ctttcattgc gcgatcagtt gcctgccacc
     1321 actgttccgg gtcttgttcc gaccagagtg gatgcgggcg cgaaacggtc agcttttccg
     1381 tttgcgaagc aaccacctca ccctgctcgt tgagcaaaat aacttttacg cccgaggtgc
     1441 caagatctat cccgatatac atatcgatcg ttccttaaaa aaatgcccgg tatcgctacc
     1501 gataaccggg ccaacggact gcacagttag ccgttatttg tcgaacagat aatgatttac
     1561 cagattttcc agttgctcct ggcgaccact ctgatgcacc ggagacaaat tatgttcctg
     1621 agcatatttg gctaaatctg ccagtgacat ttggcctttc aggatttgct ggcccaattc
     1681 gctattccag ccggaataac gctgcgcgat gcgtttatcc agctcgccat cttcaatcat
     1741 gcacgctgca attttcagcg ccagtgccat cgtatccatc gcgccgatat gaccgtaaaa
     1801 cagatcatat ttatcagtac tttgacgacg tactttggca tcgaagttca gaccaccggt
     1861 ggtgaaaccg cctgctttga gaatttcata catcaccagc gcattctctt ccacactgtt
     1921 cgggaactgg tcggtgtccc agcccagttg cgcatcgcca cggttggcgt cgacagaacc
     1981 gaacaggcca agcgcaatgg cggtggctat ttcatgatgg aaagagtgac ctgccagcgt
     2041 cgcgtggtta gcttcaatgt tcagtttaat ctctttttcc agaccaaact gtttcaggaa
     2101 gccatagact gtcgcagcat cgtaatcata ttgatgttta gtcggttctt gcggtttcgg
     2161 ttcgataagc aacgtgccct ggaagccgat tttatgttta tgctcaacca ccatttgcat
     2221 aaagcggccc agttgttcac gctcctgacg caaatcggta tttaacagcg tttcgtaacc
     2281 ttcacgaccg ccccacagga catagttttc accacccaat ttatgggttg cttccatcgc
     2341 tgtaacaact tgcgttgccg cccagctgaa gacttctgga tctgggttcg tcgccgcacc
     2401 cgcgccatag cgagggtttg taaagcagtt agcggttccc cacagcagct tcacgccgct
     2461 ctcttcttgc ttgcctgcca gaacatcaac catttgcgca aaattattga tgtactcttt
     2521 taacgacgcg ccctcaggag aaacatccac atcgtggaag caataaaatg gcacatgtaa
     2581 cttgtggaaa aactcaaatg cgacatctgc tttacgcttc gccaacgcca gtgcctcacc
     2641 aggctgctgc cacggacgat taaacgcccc cacaccaaac atatccgccc cgttccagca
     2701 gaaggtgtgc cagtagcagg cggcaaatcg caagtgctct tccatacgct tgcccaacac
     2761 cagttcgtcg ggattgtagt gacggaatgc taacgggttt gaggattttg agccttcata
     2821 acgaacgcga tcgagctggt caaaataggc ttgcatattg aactccataa tcaggtaatg
     2881 ccgcgggtga tggatgatgt cgtaatattg ggcactccct ttcagttgct caattatgtt
     2941 atttcacact gctattgaga taattcacaa gtgtgcgctc gctcgcaaaa taaaatggaa
     3001 tgatgaaact gggtaattcc tcgaagagaa aaatgcaata agtacaattg cgcaacaaaa
     3061 gtaagatctc ggtcataaat caagaaataa accaaaaatc gtaatcgaaa gataaaaatc
     3121 tgtaattgtt ttcccctgtt tagttgctaa aaattggtta cgtttatcgc ggtgattgtt
     3181 acttattaaa gctgtcctct aactacagaa ggccctacac catgaaaata aagaacattc
     3241 tactcaccct ttgcacctca ctcctgctta ccaacgttgc tgcacacgcc aaagaagtca
     3301 aaataggtat ggcgattgat gatctccgtc ttgaacgctg gcaaaaagat cgagatatct
     3361 ttgtgaaaaa ggcagaatct ctcggcgcga aagtatttgt acagtctgca aatggcaatg
     3421 aagaaacaca aatgtcgcag attgaaaaca tgataaaccg gggtgtcgat gttcttgtca
     3481 ttattccgta taacggtcag gtattaagta acgttgtaaa agaagccaaa caagaaggca
     3541 ttaaagtatt agcttacgac cgtatgatta acgatgcgga tatcgatttt tatatttctt
     3601 tcgataacga aaaagtcggt gaactgcagg caaaagccct ggtcgatatt gttccgcaag
     3661 gtaattactt cctgatgggc ggctcgccgg tagataacaa cgctaagctg ttccgcgccg
     3721 gacaaatgaa agtgttacaa ccttacgttg attccggaaa aattaaagtc gttggtgacc
     3781 aatgggttga tggctggtta ccggaaaacg cattgaaaat tatggaaaac gcgctaaccg
     3841 ccaataataa caaaattgat gctgtagttg cctcaaacga tgccaccgca ggtggggcaa
     3901 ttcaggcatt aagcgcgcaa ggtttatcag ggaaagtagc aatttctggc caggatgcgg
     3961 atctcgcagg tattaaacgt attgctgccg gtacgcaaac tatgacggta tataaaccta
     4021 ttacattgct ggcaaatact gccgcagaaa ttgccgttga attgggcaat ggtcaggagc
     4081 caaaagcaga tacttcacta aataatggcc tgaaagatgt cccctcccgc ctcctgacac
     4141 cgatcgatgt gaataaaaac aacatcaaag atacggtaat taaagacgga ttccacaaag
     4201 agagcgagct gtaagcgtta cgccccagcg cggagcgggg gcgtgatttc tctccatgcc
     4261 gcgtgaatga attggcttag gtggagtcgc tatgccttat ctacttgaaa tgaagaacat
     4321 taccaaaacc ttcggcagtg tgaaggcgat tgataacgtc agcttgcggt tgaatgctgg
     4381 cgaaatcgtc tcactctgtg gggaaaatgg gtctggtaaa tcaacgctga tgaaagtgct
     4441 gtgtggtatt tatccccatg gctcctacga aggcgaaatt atttttgcgg gagaagagat
     4501 tcaggcgagt cacatccgcg ataccgaacg caaaggtatc gccatcattc accaggaatt
     4561 ggccctggtg aaagaattga ccgtgctgga aaatatcttc ctgggtaacg aaataaccca
     4621 caatggcatt atggattatg acctgatgac gctacgctgt cagaagctgc tcgcacaggt
     4681 cagtttatcc atttcacctg atacccgcgt tggcgattta gggcttgggc aacaacaact
     4741 ggttgaaatt gccaaggcac ttaataaaca ggtgcgcttg ttaattctcg atgaaccgac
     4801 agcctcatta actgagcagg aaacgtcggt tttactggat attattcgcg atctacaaca
     4861 gcacggtatc gcctgtattt atatttcgca caaactcaac gaagtcaaag cgatttccga
     4921 tacgatttgc gttattcgcg acggacagca cattggtacg cgtgatgctg ccggaatgag
     4981 tgaagacgat attatcacca tgatggtcgg gcgagagtta accgcgcttt accctaatga
     5041 accacatacc accggagatg aaatattacg tattgaacat ctgacggcat ggcatccggt
     5101 taatcgtcat attaaacgag ttaatgatgt ctcgttttcc ctgaaacgtg gcgaaatact
     5161 gggtattgcc ggactcgttg gtgccggacg taccgagacc attcagtgcc tgtttggtgt
     5221 gtggcccgga caatgggaag gaaaaattta tattgatggc aaacaggtag atattcgtaa
     5281 ctgtcagcaa gccatcgccc aggggattgc gatggtcccc gaagacagaa agcgcgacgg
     5341 catcgttccg gtaatggcgg ttggtaaaaa tattaccctc gccgcactca ataaatttac
     5401 cggtggcatt agccagcttg atgacgcggc agagcaaaaa tgtattctgg aatcaatcca
     5461 gcaactcaaa gttaaaacgt cgtcccccga ccttgctatt ggacgtttga gcggcggcaa
     5521 tcagcaaaaa gcgatcctcg ctcgctgtct gttacttaac ccgcgcattc tcattcttga
     5581 tgaacccacc aggggtatcg atattggcgc gaaatacgag atctacaaat taattaacca
     5641 actcgtccag cagggtattg ccgttattgt catctcttct gaattacctg aagtgctcgg
     5701 ccttagcgat cgtgtactag taatgcataa agggaaacta aaagccaacc tgataaatca
     5761 taacctgact caggagcagg tgatggaagc cgcattgagg agcgaacatc atgtcgaaaa
     5821 gcaatccgtc tgaagtgaaa ttggccgtac cgacatccgg tggcttctcc gggctgaaat
     5881 cactgaattt gcaggtcttc gtgatgattg cagctatcat cgcaatcatg ctgttcttta
     5941 cctggaccac cgatggtgcc tacttaagcg cccgtaacgt ctccaacctg ttacgccaga
     6001 ccgcgattac cggcatcctc gcggtaggaa tggtgttcgt cataatttct gctgaaatcg
     6061 acctttccgt cggctcaatg atggggctat taggtggcgt cgcggcgatt tgtgacgtct
     6121 ggctaggctg gcctttgcca cttaccatca ttgtgacgtt ggttctggga ctgcttctcg
     6181 gtgcctggaa cggatggtgg gtcgcgtacc gcaaagtccc ttcatttatt gtcactctcg
     6241 cgggcatgtt ggcatttcgc ggcatactca ttggcatcac caacggcacg actgtatccc
     6301 ccaccagcgc cgcgatgtca caaattgggc aaagctatct ccccgccagc accggcttca
     6361 tcattggcgc gcttggctta atggcttttg ttggctggca atggcgcgga agaatgcgcc
     6421 gtcaggcttt gggtttgcag tctccagcct ctaccgcagt agtcggtcgc caggctttaa
     6481 ccgctatcat cgtattaggc gcaatctggc tgttgaatga ttaccgtggc gttcctactc
     6541 ctgttctgct gctgacgttg ctgttactcg gcggaatgtt tatggcaacg cggacagcat
     6601 ttggacgacg catttatgcc atcggcggca atctggaagc agcacgtctc tccggaatta
     6661 acgttgaacg caccaaactt gccgtgttcg ctattaacgg attaatggta gccatcgccg
     6721 gattaatcct cagttctcga cttggcgctg gttcaccttc tgcggggaat atcgccgagc
     6781 tggacgcaat tgcagcatgc gtgattggcg gcaccagtct ggcaggcggc gtcgggagcg
     6841 tggctggagc ggtaatgggg gcattcatca tggcttcact ggataacggt atgagtatga
     6901 tggatgtacc gaccttctgg cagtatatcg ttaaaggtgc gattctgttg ctggcagtat
     6961 ggatggactc cgcaaccaaa cgccgttctt gattttgata aaaattttct caaagccggt
     7021 aacgtattac cggttttgag tttttgcatg attcagcagg aaaagaacca tgtttactaa
     7081 acgtcaccgc atcacattac tgttcaatgc caataaagcc tatgaccggc aggtagtaga
     7141 aggcgtaggg gaatatttac aggcgtcaca atcggaatgg gatattttca ttgaagaaga
     7201 tttccgcgcc cgcattgata aaatcaagga ctggttagga gatggcgtca ttgccgactt
     7261 cgacgacaaa cagatcgagc aagcgctggc tgatgtcgac gtccccattg ttggggttgg
     7321 cggctcgtat caccttgcag aaagttaccc acccgttcat tacattgcca ccgataacta
     7381 tgcgctggtt gaaagcgcat ttttgcattt aaaagagaaa ggcgttaacc gctttgcttt
     7441 ttatggtctt ccggaatcaa gcggcaaacg ttgggccact gaacgcgaat atgcatttcg
     7501 tcagcttgtc gccgaagaaa agtatcgcgg agtggtttat caggggttag aaaccgcacc
     7561 agagaactgg caacacgcgc aaaatcggct ggcagactgg ctacaaacac tgccaccgca
     7621 aaccgggatt attgccgtta ctgacgcccg ggcgcggcat attctgcaag tatgtgaaca
     7681 tctacacatt cccgtaccgg aaaaattatg cgtgattggc atcgataacg aagaactgac
     7741 ccgctatctg tcgcgtgtcg ccctttcttc ggtcgctcag ggcgcgcggc aaatgggcta
     7801 tcaggcggca aaactgttgc atcgattatt agataaagaa gaaatgccgc tacagcggat
     7861 tttggtccct ccagttcgcg tcattgaacg gcgctcaaca gattaccgtt cgctgaccga
     7921 tcccgccgtt attcaggcca tgcattacat tcgtaatcac gcctgtaaag ggattaaagt
     7981 ggatcaggta ctcgatgcgg tcgggatctc gcgctccaat cttgagaagc gttttaaaga
     8041 agaggtgggt gaaaccatcc atgccatgat tcatgctgag aagctggaga aagcgcgcag
     8101 tctgctgatt tcaaccacct tgtcgatcaa tgagatatcg caaatgtgcg gttatccatc
     8161 gctgcaatat ttctactctg tttttaaaaa agcatatgac acgacgccaa aagagtatcg
     8221 cgatgtaaat agcgaggtca tgttgtagcg atgatgagaa ttgtcggcgt cgcatcaggt
     8281 aactcagcac aaccacctga tgcgatcaaa agccttatcc gaccgacaag tcggataagg
     8341 ctcgaggaaa tacaattaca tatgcgccgc gattaaccgt tggttatcct ggtacattgc
     8401 gaacaggtag ttgttataac tcttcccttt ggtcgagtag cccttcagct tgtgaatcat
     8461 cgctgtggca gtcacttcct gatcagcttt acgcagctgc gcacgcgatt tacggaacga
     8521 agagtaagcc ggatgcgtat tgaggttagt gacataggcg ctcaccgatt ctttgacaga
     8581 actaaactgt gagtaccctt tcactttacc tggcgcattg gtacaacgtc ctttcatgca
     8641 tttcatgccg aaaagattgt tgttgttgcg cgccagcttc gacgttcccc aaccgctttc
     8701 tgctgcagcc atcgtcgcca ccatactggt agggataatg tctacgcgtt caagcaaggt
     8761 attccacggg atttttcgcg tattaccaga ccacttcacc ttgtagcgtt tggcgatgtc
     8821 tttcagacgc gcacgctcag caggtgacca ttggccctga tactgttttg aaatcagcca
     8881 gttacgttct gcagtaatgg ctgcattttg gctggtaatg taaggcatta cggtccggag
     8941 aaacgccttt ttccttgatg ttccggaagg gtattttcgc aaatcaggaa gtgaactact
     9001 ctttgcacta ttgcgagaat actcttgttt actgcttacc tgtttattac tagctttagt
     9061 taagtgggac ttttgactcg ctgttgttgt gtgcgtcttc gctaacacct cactcgaaaa
     9121 caccagagtg agtaacataa gaatcatcgc cccatatcgt cgtatgggag tcaaaatcat
     9181 caggtctcct ggtcggattt aatcattcca acaccttata tttttcacaa atttgagagt
     9241 tgaatctcaa atcatatcaa aaatagctgt caagagcacc ccaaggaata gtccaaatct
     9301 gaaactatgt cacgagttaa cgattcagat tggcgctaaa tcgcagaaaa tgtggggttt
     9361 atcgcaaaat tcagccgttt tttgcgcgcg atcgctcacc cttgcttctc atcctgtgga
     9421 cttaccgctc agggatgagt tttgtctggc ttatcgctgg caaactgtct gaaatcgcag
     9481 caataaggac tcatccacca tgaaactcgc cgcctgtttt ctgacactcc ttcctggctt
     9541 cgccgttgcc gccagctgga cttctccggg gttccctgcc tttagcgaac agggaacggg
     9601 aacatttgtc agccacgcgc agttgcccaa aggtacgcgt ccactcacgc taaattttga
     9661 ccagcagtgc tggcagcctg cagatgcgat aaaactcaat cagatgcttt ccctgcaacc
     9721 ttgtagcaac acgccgcctc aatggcgatt gttcagggac ggcaaatata cgctgcaaat
     9781 agacacccgc tccggtacgc caacattgat gatttccatc cagaacgccg ccgaaccggt
     9841 agcaaacctg gtccgtgaat gcccgaaatg ggatggatta ccgctcacgc tggatgtcag
     9901 cgccactttc ccggaaggag ccgccgtacg ggattattac agccagcaaa ttgcgatagt
     9961 gaagaacggt caaataacgt tacaacccgc tgctaccagc aacggtttac tcctgctgga
    10021 acgggcagaa actgacgcct ctgccccttt cgactggcat aacgccacgg tttactttgt
    10081 gctgacagat cgtttcgaaa acggcgatcc cagtaatgac cagagttacg gacgtcataa
    10141 agacggtatg gcggaaattg gcacttttca cggcggcgat ttacgcggcc tgaccaacaa
    10201 actggattac ctccagcagt tgggcgttaa tgctttatgg ataagcgccc catttgagca
    10261 aattcacggc tgggtcggcg gcggtacaaa aggcgatttc ccgcattatg cctaccacgg
    10321 ttattacaca caggactgga cgaatcttga tgccaatatg ggcaacgaag ccgatctacg
    10381 gacgctggtt gatagcgcac atcagcgcgg tattcgtatt ctctttgatg tcgtgatgaa
    10441 ccacaccggc tatgccacgc tggcggatat gcaggagtat cagtttggcg cgttatatct
    10501 ttctggtgac gaagtgaaaa aaacgctggg tgaacgctgg agcgactgga aacctgccgc
    10561 cgggcaaacc tggcatagct ttaacgatta cattaatttc agcgacaaaa caggctggga
    10621 taaatggtgg ggaaaaaact ggatccgtac cgatatcggc gattacgaca atcctggatt
    10681 cgacgatctc accatgtcgc tagccttttt gccggatatc aaaaccgaat caactaccgc
    10741 ttctggtctg ccggtgttct ataaaaacaa aacggatacc cacgctaaag ccatcgacgg
    10801 ctttacccct cgcgattact taacccactg gttaagtcag tgggtccgcg actatgggat
    10861 tgatggtttt cgggtcgata ccgccaaaca tgttgagttg cccgcttggc agcaactgaa
    10921 aaccgaagcc agcgccgcgc ttcgcgaatg gaaaaaagct aaccccgaca aagcattaga
    10981 tgacaaacct ttctggatga ccggtgaagc ctggggccac ggcgtgatgc aaagtgacta
    11041 ctatcgccac ggcttcgatg cgatgatcaa tttcgattat caggagcagg cggcgaaagc
    11101 tgtcgattgt attgcgcaga tggatacgac ctggcagcaa atggcggaga aattgcaggg
    11161 tttcaacgtg ttgagctacc tctcgtcgca tgatacccgt ctgttccgtg aagggggcga
    11221 caaagcagca gagttattac tattagcgcc aggcgcggta caaatctttt atggcgatga
    11281 atcctcgcgt ccgttcggtc ctacaggttc tgatccgctg caaggtacac gttcggatat
    11341 gaactggcag gatgttagcg gtaaatctgc cgccaacgtc gcgcactggc agaaaatcag
    11401 ccagttccgc gcccgccatc ccgcaattgg cgcgggcaaa caaacgacac tttcgctgaa
    11461 gcagggctac ggctttgttc gtgagcatgg cgacgataaa gtgctggtca tctgggctgg
    11521 gcaacagtga cttttccggc ttcccgttcg tcagtacctc gggaagccgc caaccaggat
    11581 aaaatgtcag ccctaatcag cgttgcagga taaagcaccg ctcactcttc aacagaccga
    11641 tttgcacccc agcaaatgta gcgttattgt taccttcctt gctacagagt tcgacagata
    11701 tcccgctatg acattctccc tttttggtga caaatttacc cgccactccg gcattacgct
    11761 gctgatggaa gatctgaacg acggtttacg cacgcctggc gcgattatgc tcggcggcgg
    11821 taatccggcg cagatcccgg aaatgcagga ctacttccag acgctactga ccgacatgct
    11881 ggaaagtggc aaagcgactg atgcactgtg caattacgac ggtccacagg ggaaaacgga
    11941 gctactcaca ctgcttgccg gaatgctgcg cgagaagttg ggttgggata tcgaagcaca
    12001 gaatattgca ctaacaaacg gcagccagag cgcgtttttc tacttattta atctgtttgc
    12061 cggacgccgt gccgatggtc gggtcaaaaa agtgctgttc ccgctggcac cggaatacat
    12121 tggctacgct gacgccggac tggaagaaga tctgtttgtc tctgcgcgtc cgaatattga
    12181 actgctgccg gaaggccagt ttaaatacca cgtcgatttt gagcatctgc atattggcga
    12241 agaaaccggg atgatttgcg tctcccggcc gacgaatcca acaggcaatg tgattactga
    12301 cgaagagttg ctgaagcttg acgcgctggc gaatcaacac ggcattccgc tggtgattga
    12361 taacgcttat ggcgtcccgt tcccgggtat catcttcagt gaagcgcgcc cgctatggaa
    12421 tccgaatatc gtgctgtgca tgagtctttc caagctgggg ctgcctggct cccgctgcgg
    12481 cattatcatc gccaatgaaa aaatcatcac cgccatcacc aatatgaacg gcattatcag
    12541 cctggcacct ggcggtattg gtccggcgat gatgtgtgaa atgattaagc gtaacgatct
    12601 gctgcgcctg tctgaaacag tcatcaaacc gttttactac cagcgtgttc aggaaactat
    12661 cgccatcatt cgccgctatt taccggaaga tcgctgccta attcataaac cagaaggagc
    12721 cattttcctc tggctttggt ttaaggattt gcccattacg accgagcagc tctatcagcg
    12781 cctgaaagca cgcggcgtgc tgatggtgcc ggggcacaac ttcttcccag ggctggacaa
    12841 accgtggccg catacgcatc aatgtatgcg catgaactac gtaccagagc cggagaaaat
    12901 tgaggcgggg gtgaagattc tggcggaaga gatagaaaga gcctgggctg aaagtcacta
    12961 aaaattgcct gatgcgctac gcttatcagg cctacatgat ttctgcaata tatttaattt
    13021 gcaagaattt gtgggccgga taaggcgttt acgccgcatc tggcataaac aaagcgcact
    13081 attccggcgt tgagaaacgc cggaaaaacg tttcaaacag gctgctgccg tagccgatgc
    13141 cgctgcactt tctcgacatc catgcactgc aacgcctttg tcgggcaggc ttcaacacag
    13201 gccggtcccg tctcccgatg ccagcacaga tcgcacttaa tcgccctcgc ctttttgcgc
    13261 gaagaaacga cctccatcgc accaaacggg caagccagca tacagctttt acagccaatg
    13321 caacgtgttt gttcaacgaa aatatgccca tgttcgcggc ttatcgcgtc aacagggcag
    13381 acattcgcgc acggtgcatc ttcacactga tgacaggcta ctgccgtggt ccagctgtgg
    13441 tctttaatga cacgaatacg ggaaataaac tcgtctggtg acaacgcagc gcaatcctga
    13501 ttctcctgat gcgacactgc gcaagccact tcacaggtac ggcaaccgat acatttcgtc
    13561 gcatccgcaa taataaaccg gttcatgatc actcctttgc actaaaacag cagagtgcca
    13621 gagagcgcgt ccctactgct ttgatccaac gaaggaaatg ttaaagtttt ggcacttgtc
    13681 caaagcgtta tgtgatcgcg ccctggtcgt ccctgacagt aaatcccagt tcattagaaa
    13741 tagcctgcgc ggtttcacgc agtggtttca ggagattttt ctcccccacc tgtttcagac
    13801 gtgatgtcga aagcgaaatc gccccggcac tacgaattga tatgttcctt gctgtaacgc
    13861 cgcttccacg ctgctggcgt taaaccggtg tgtttgcgaa aaatctgccg gaaatagcca
    13921 acgtcattaa acccgcacaa cgccgccact tccgtgagcg atcgcgcttc gctaattaac
    13981 agcttttcgg cttcgcgcac ccgctggcga tggattgctt ctgttaacgt cagatgaaac
    14041 acccgccggt agacgcgccc aagataatcg gcattgcagt acagctcgcg ggccagaagc
    14101 gccgtagaaa gcgggcgatg atattgcgtg cggatcagtt tctgcgcttt ccaggcaagg
    14161 gtggtgcctg cgccatcggc ctgttcatca agacgcgcgt cacgggtgag ctgctgcaaa
    14221 atgagcagca caataaactc cagcgccgga gtgcgttgat gcttttcctg ttcgcttaag
    14281 aactggcgaa acagggccac cagcgcctgc ggatcgacaa ccttcgtttg ttgcggcacg
    14341 ctaagatgag taagccagcg agggtcgttg tccggcgctt taacctcaaa atgcagccag
    14401 taaaatttga gatcggcggg aaaatcctcc accccaacat gttgtcggtt cggccagagt
    14461 aataaactct ctcccgcatc gaccagaaat atccgttctt cttcctgaat cgttaatcga
    14521 cctttttcga caaagataat ttcccatgac tccagccgcc ttgcagaatg cctccctacg
    14581 ccacgggaaa taaataatcc accattttgc actttaatcg gtaacgtcat ggataattcg
    14641 agcattgata tgtaatttcc cgcttattca ttaatgatta aacacattac cttttgatta
    14701 ttaatcaaaa acattaattt cacctgatcc atttcacact tttaaattca ggtcggaatc
    14761 gtcacctttt tttactcttt tctcgccttg ttgatattcc cgttcacggc agaattaatc
    14821 aggtcatttc aataaaacta aaaattgctc cctattcacg aggaatatct atgacctcta
    14881 caccgattac taccgctgat atcgctaaag gtaaaattga cgatgcgtta tctgtacggg
    14941 aaaaaatagg ctacggcctg ggtgacgcag gcggcaccgt aataacttgc ctgatcatga
    15001 attttctcac ctttttctac accgacgttt ttggattaac tccggcgctg gttggcacgc
    15061 tgtttattgc actgcgcgtg tttgatgcca tctccgaccc ggtgatgggc gtcattgccg
    15121 accggacgca aagccgatgg gggcgctttc gtccgtggca gctatggatt gccattccca
    15181 tcggcattat cggcatcctg acgttcaccg tgccagatgc cagcatggga gtaaaaatcg
    15241 cctgggcgtt cggtacttac ctgctccttt cagtcggtta taccgccatc aacgtaccgt
    15301 actgcgcgcg gatcaacacc atgaccaccc gccacaatga agtgatctcc tgccagttct
    15361 ggcgattcgt tctctgcggc gtagcgggat ttctggtttc ggtaggctta ccgtggatgg
    15421 tagctctctt cggtcagggc aacgctgcac gcggctatca actgggcgtc ggggtattgt
    15481 gcgccattgc cgtggtgatg ttcctgtgct gtttcttctg ggttcgtgaa cgggtgccgc
    15541 tctccacaat ggggaaattt accctgcgcg aacatcttgc cgggctgcgg aacaacgacc
    15601 aactgctgct gatgctggtc atgtctttcc tgctgattaa cgtctttaac attcgcggcg
    15661 gtgggtatat gtacttcatt acctacgtct tacaaggcag cacgggctac acgtcgctgt
    15721 tcttcaccat ggtcaccttc gcctccatta tcggctcggt gattgtcagc ccgttaacgc
    15781 ggcgtttcga taccgtcaaa atttattact acaccaacct gctcctcgct gcactggcgg
    15841 tgttgatgtg gttcctgccc tccggcccgg cttatcaaac gctgtggctg gcggtgatcc
    15901 tcggtaatgg cgtgattctt ggcttcacat tgccactgca cttctcattg atggcctttg
    15961 ccgatgacta cggcgagtgg aaaacccacg tacgttcttc cggcatgaac ttcgccttca
    16021 atctgttttt catcaagctg gcctgggcct ccagcgccgg gatcatcagc ctgctgttta
    16081 tttttgtcgc ctaccagcct ggcgtggaaa accagaccgc cagttcgctt ggcgggatcg
    16141 cggcgatgga aacattactg cctgcgctat tccacctgct gctggcaggg gcgatccgct
    16201 tttgcaaact caataatcct atgatgtcac gcatagctac cgacctgcgt cagcgtcatg
    16261 tacagcctta aggagcacaa gatgaacatt tcggaagtcg atctgcataa actgacggtc
    16321 agcgatccgt tcctcggtca gtaccaacaa ctggtccgcg acgtggtgat tccttatcag
    16381 tgggatgcct tgaacgatcg tatcccagaa gcggaaccca gccatgcgat tgaaaacttt
    16441 cgcattgccg ccggacttca ggagggtgaa ttttacggga tggtgtttca ggacagcgac
    16501 gtcgccaaat ggctggaagc ggtagcctgg tcgctgtgcc agaagccgga cgccgaactg
    16561 gaaaaaaccg ccgacgaggt aatcgaactg atcgcctccg cccaatgcga agacggctat
    16621 ctcaatactt actttacggt aaaagcaccc gaagaacgct ggagcaatct ggcggagtgt
    16681 catgaacttt actgcgcagg tcatctgatt gaagccggag tcgccttctt ccaggccacg
    16741 ggaaaacggc gcttgctggg ggtcgtttgc cgcctggccg atcatatcga cagcgtattt
    16801 ggtccagatg aaagtaagtt acacggttat cctggtcacc cggaaattga actggcacta
    16861 atgcgcctgt atgaagtgac tgaagagccg cgctacctgg cgctgacgaa ctattttgtc
    16921 gaacagcgtg gtgcgcaacc gcactattac gaccaggaat atgaaaagcg cgggcagaca
    16981 tcgcactggc acacctacgg cccggcgtgg atggtgaaag acaaagccta cagccaggca
    17041 catttgcccc ttgcgcaaca gcaaaccgcc atcggtcacg cggtacgttt tgtctatctg
    17101 atgaccggcg tcgcgcatct cgcgcgttta agtcacgatg acagcaagcg tcaggactgc
    17161 ctgcggctgt ggaacaatat ggcccagcgt cagttatata ttaccggcgg catcggctca
    17221 caaagcagcg gcgaagcgtt cagcagcgat tacgatctgc cgaatgacac ggtttacgcc
    17281 gaaagttgtg cttccatcgg cctgatgatg ttcgcccgac gaatgctgga aatggaaggc
    17341 gacagtcaat atgccgatgt gatggagcgc gcactgtaca acactgtgct cggcggcatg
    17401 gcattggatg gcaaacattt cttctatgtg aatccactgg aagtacatcc aaaatcgctg
    17461 aaattcaacc atatctacga tcacgttaaa ccgatccgcc agcgttggtt tggctgcgct
    17521 tgttgtccgc caaatatcgc ccgcgtgctg acctcgattg gtcattatct ctacacgccg
    17581 cgtgaagatg cgttgtatat caacatctac gcaggaaaca gcatggaagt gccggtagaa
    17641 aatggcacgc tgcgcctgcg ggttagcggg aactatccgt ggcaggagca ggtgacgatt
    17701 gcggttgaat cgccccagcc ggtacgtcat acgctggctt tacgtctgcc ggactggtgc
    17761 acacagccgc agatcatatt gaatggggaa gaggtcgagc aggatattcg taaagggtat
    17821 ttgcacatta cccgcgaatg gcaggagggc gatacgctga atctgacttt gccgatgccg
    17881 gtacgccgcg tttacggtaa cccgctggtg cgtcacgtcg ccggaaaagt ggcgattcag
    17941 cgcggcccgc tggtgtattg cctggaaaag gccgacaacg gcgagtcact gcataatctg
    18001 tggctgccca ccgatgcgcc atttacgaca tttgaaggca agggattgtt tagccataag
    18061 atcttaatcc aggcaccggg ttaccggtat gaacagagca atccagagca gcaaccgctg
    18121 tggcattacg acagcgcgcc agccaaacgc cagacgcaaa ctctgacctt tatcccgtgg
    18181 tttagctggg ccaaccgggg tgaaggcgaa atgcggatct gggtgaatga ggaaaagcat
    18241 tgccatccgt aggttggata aggcactcgc gccgcatcca acgtaaggag cacattgcct
    18301 gatgcgacgc ttaacacgtc ttatcaggca tacaagtcgg tgcgagagcg taggccagcc
    18361 gcaccgacat ccaccggtga attaaaaacg ccagctcacg ccggtgacaa aggtaaaact
    18421 ctcattacga tcaatcatcg ggctgtcggt aatctcttcc ggcagctcgc tgtatcctgc
    18481 gctcgccatc aatacgacgt gctctcctat cgggtatttt gccgtcaggc tgacataggg
    18541 cacccaggca tcctgcgcgg aataacttgc cagaccgcta cgacgggact cactctctga
    18601 aatgccatag taatagtcac tgaaattctc gtcataatag agaacgccca gcgcaggtgt
    18661 cagcgacaga cgacctatct gcattttgtg gaataccgat agctccccca cccagccgtt
    18721 gctgttatcc agaacgtccg cagctgcaga ggctttcacg gttccccaac gctcgtggtg
    18781 ataccaggca acccccgcca tagccgtact atcacgctta tcaagctgtt gcatggcata
    18841 atcgtcatta tcggcagggt caaattccag cggcatccag gatgcggtca ggctaagttc
    18901 atttttttca ctttgcgaca gaataaaacc taacgtggtc tgacgaacat aaaaagaatc
    18961 accttcataa ctaatcagcg gaattgcctt tgtattttca ttataaccgc gataaggcga
    19021 ttcattataa gccgcaccag caccaatgga taattcagaa gcagttgcgc ttgccataaa
    19081 aggcaacgca aataacgcca caatattgcg gttaattaac atgtccggta ttccatttta
    19141 aa
//
LOCUS       DAZFBR010000091        19043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFBR010000091 DAZFBR010000000
VERSION     DAZFBR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.091"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(99..464)
                     /gene="yhaH"
                     /locus_tag="ACHJZ2_003496"
     CDS             complement(99..464)
                     /gene="yhaH"
                     /locus_tag="ACHJZ2_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4569542.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(756..1742)
                     /gene="yqjG"
                     /locus_tag="ACHJZ2_003497"
     CDS             complement(756..1742)
                     /gene="yqjG"
                     /locus_tag="ACHJZ2_003497"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO4569543.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDVRLG"
     gene            complement(1812..2204)
                     /gene="yqjF"
                     /locus_tag="ACHJZ2_003498"
     CDS             complement(1812..2204)
                     /gene="yqjF"
                     /locus_tag="ACHJZ2_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4569544.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2390..2689)
                     /gene="yqjK"
                     /locus_tag="ACHJZ2_003499"
     CDS             complement(2390..2689)
                     /gene="yqjK"
                     /locus_tag="ACHJZ2_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO4569545.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2679..3083)
                     /gene="yqjE"
                     /locus_tag="ACHJZ2_003500"
     CDS             complement(2679..3083)
                     /gene="yqjE"
                     /locus_tag="ACHJZ2_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4569546.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3086..3391)
                     /gene="yqjD"
                     /locus_tag="ACHJZ2_003501"
     CDS             complement(3086..3391)
                     /gene="yqjD"
                     /locus_tag="ACHJZ2_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO4569547.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3429..3797)
                     /gene="yqjC"
                     /locus_tag="ACHJZ2_003502"
     CDS             complement(3429..3797)
                     /gene="yqjC"
                     /locus_tag="ACHJZ2_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO4569548.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3944..4327)
                     /gene="mzrA"
                     /locus_tag="ACHJZ2_003503"
     CDS             complement(3944..4327)
                     /gene="mzrA"
                     /locus_tag="ACHJZ2_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO4569549.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4331..4993)
                     /gene="yqjA"
                     /locus_tag="ACHJZ2_003504"
     CDS             complement(4331..4993)
                     /gene="yqjA"
                     /locus_tag="ACHJZ2_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO4569550.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5015..5098)
                     /gene="yqiM"
                     /locus_tag="ACHJZ2_003505"
     CDS             complement(5015..5098)
                     /gene="yqiM"
                     /locus_tag="ACHJZ2_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO4569551.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5338..6114)
                     /gene="exuR"
                     /locus_tag="ACHJZ2_003506"
     CDS             complement(5338..6114)
                     /gene="exuR"
                     /locus_tag="ACHJZ2_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO4569552.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6244..7542)
                     /gene="exuT"
                     /locus_tag="ACHJZ2_003507"
     CDS             complement(6244..7542)
                     /gene="exuT"
                     /locus_tag="ACHJZ2_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO4569553.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            8025..9437
                     /gene="uxaC"
                     /locus_tag="ACHJZ2_003508"
     CDS             8025..9437
                     /gene="uxaC"
                     /locus_tag="ACHJZ2_003508"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO4569554.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9452..10939
                     /gene="uxaA"
                     /locus_tag="ACHJZ2_003509"
     CDS             9452..10939
                     /gene="uxaA"
                     /locus_tag="ACHJZ2_003509"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO4569555.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            11022..11573
                     /gene="ygjV"
                     /locus_tag="ACHJZ2_003510"
                     /pseudo
     CDS             11022..11573
                     /gene="ygjV"
                     /locus_tag="ACHJZ2_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
     gene            complement(11578..12822)
                     /gene="sstT"
                     /locus_tag="ACHJZ2_003511"
     CDS             complement(11578..12822)
                     /gene="sstT"
                     /locus_tag="ACHJZ2_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO4569556.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13221..14186)
                     /gene="alx"
                     /locus_tag="ACHJZ2_003512"
     CDS             complement(13221..14186)
                     /gene="alx"
                     /locus_tag="ACHJZ2_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO4569557.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            complement(14470..15456)
                     /gene="ygjR"
                     /locus_tag="ACHJZ2_003513"
     CDS             complement(14470..15456)
                     /gene="ygjR"
                     /locus_tag="ACHJZ2_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4569558.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15535..16227)
                     /gene="ygjQ"
                     /locus_tag="ACHJZ2_003514"
     CDS             complement(15535..16227)
                     /gene="ygjQ"
                     /locus_tag="ACHJZ2_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO4569559.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(16304..16807)
                     /gene="ygjP"
                     /locus_tag="ACHJZ2_003515"
     CDS             complement(16304..16807)
                     /gene="ygjP"
                     /locus_tag="ACHJZ2_003515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO4569560.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16892..18028
                     /gene="rlmG"
                     /locus_tag="ACHJZ2_003516"
     CDS             16892..18028
                     /gene="rlmG"
                     /locus_tag="ACHJZ2_003516"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO4569561.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18312..18626
                     /gene="higB"
                     /locus_tag="ACHJZ2_003517"
     CDS             18312..18626
                     /gene="higB"
                     /locus_tag="ACHJZ2_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFO4569562.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            18623..19039
                     /gene="higA"
                     /locus_tag="ACHJZ2_003518"
     CDS             18623..19039
                     /gene="higA"
                     /locus_tag="ACHJZ2_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO4569563.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         4882 a         4919 c         4821 g         4421 t
ORIGIN      
        1 cgacattgaa atattttaaa taaacattag taaattattc attcatcaac atgatgataa
       61 aagccggatg agtcagacat ccggcgagca gatttgtttt aaggttccag ttttggatca
      121 ggtccaaagc ggttttcgcc tggcgtaccc gcctggcagt taaagacgat gataataagc
      181 cagccgataa acgggattaa gaatagcagt gcccaccacg ccgagcggtc ggtgtcgtgc
      241 aggcggcgga actgaactgc ccaccacggt aaaaacacca gaataccgta gattgtcgtc
      301 aggatgcctt cgccaccagc gcgttgccag cctaacattt tatccagcag ccccagcacg
      361 aacgtaaaga tgatgttgac cagaataaac atccagtact ctttgcgccg cgcacgaccc
      421 cggaaaccga cataattttt tagtactttc agataccagt ccatttttgc ctcaattttg
      481 ctcaatcact tgtttttaaa gcagcaaatt aagaatagac gtgaatgaaa tggcagtaaa
      541 tattcgttca aggaattaat aaagtgctgt gaaggcgcgc taagactgat gacaagcgca
      601 aaattgccag atgcgctacg cttatctggc ccgcgcggtc attgcaacat attgaatata
      661 aaagttttgt aggtttgata aggcgttcat gccgcatccg acatgaacaa catgcactta
      721 gctaattatc agaggcacct cgtaaggtgc tttttttaac ctaagcgaac atctcgtcca
      781 tgcggttcat cgagatcctg ccacggacca attgaaataa tccgcgtagg gttgatggtc
      841 ttatgactgc ggaagtaatg attacggata tgatcgaaat tgactgtttc ggcaattccc
      901 ggcatctggt agatatcacg caggaagcca tacagattaa ggtaatcgct gatgcggtgc
      961 ttatcacact tgaagtgggt cacatacact ggatcaaaac ggaccagcgt ggtccacagg
     1021 cgaatatcgg cttcggttag ctggttgccg gtcaggtaac gatgctgacc taaaatctgt
     1081 tccagtcgcg ccagcgattc aaacactttc tccaccgctt cgtcgtaggc ttgctggctg
     1141 gtggcaaaac cagctttata cacgccgttg ttaacggtgt cataaatcca gccgttaagt
     1201 tcgtcaattt tcgtttgcag ggctggtggg tagtaatcac ccgctttcgc gcccagcgca
     1261 tcaaacgcgg tattaaacat gcggatgatt tctgctgatt cgttgctgac gatggtgtgg
     1321 ttctttttgt cccacagcac gggaacagta actcgtccgc tgtagtgtgg atcggcgtgg
     1381 agataaagct gatacaaaaa ttcatgttga tagagcgtgt ctccggttgc tcccggaaaa
     1441 ctgtcatcaa aggtccagcc gttttccagc atcagcgggt tcactacgga aacggaaata
     1501 aacggttcca gtcctttgag tttgcgcatg atcagcgtgc ggtgcgccca cgggcaggcg
     1561 agtgaaacat agagatgata acgatctttc tctgcgataa aaccgcctgt gccagtgggg
     1621 ccaggagcgc catcggcagt gagccagtta cgaaatgcgg aagctgaacg ttgaaattta
     1681 ccgccggtag atttggtatc gtaccaggtg tcatgccaga cgccgtcaat cagttgaccc
     1741 attgttctct cctccaatag caaaaacgag gagcttcctc ctcgtttttt atcgttaagt
     1801 atagcgcgtg cttaccactt tttattcagc aggcggtcga tgctatacgc gcccggaccg
     1861 gtaattgcca gcagcaggaa tccgccagaa attgtcaggt ttttcatgaa catcagcgag
     1921 ttgacgcctt cagcaaagtt gctgtgaaat aaaaatgccg tcagcagcgt aaagcccgca
     1981 gtaaacaggg ctgtggtgcg agtcaggaaa ccgaacagga ttgccagacc accaccaaac
     2041 tcaagcagaa tcaccagcgg cagcataaaa cccgggacgc ccatcgcttc catatattgt
     2101 tgggtacccg cgtaaccagt aatttttccc cagcctgcgg taataaacag aatcggcatt
     2161 aaaatgcgcg ctaccagtac accaacatct tctaattttt tcatcatact ctccactgcg
     2221 ccactcaggc tgctgattgt ttttttgtgc aagggcgcgg tattagcgtc gttgctgtcg
     2281 atggagagaa tcataaacgt ggtgaatgat gattgttagc aaggaaaact gtcaaaaatc
     2341 ttcaaaaaat ttgatgggta aggccggaat ggctccggcc agagggaagt taaccgcgaa
     2401 gctgttgctg cttgagggtc gttttaacca gacgccaggc gctccatacg ccaaaaccgc
     2461 gtctggccca gcggaccagc atattaggat ggcgaatcgt ccagatcgcc atcacgctac
     2521 tgccaaccag cgcccaggag cgcagactta gcagcatatt ccagcgacga tcgtaagcgc
     2581 ctgttgcctc cagccattca cgacgactgg cagaaagatc cagccgttgt tgctggatct
     2641 ggctaagcag ttgcgcctta cgtcgttcac gttcgacttt actgctcacg ggactcctcc
     2701 tcgagcagct gccgatcgtt tgccagctca tggcgtgtat ggcgcagcaa ggtggattta
     2761 cgcgatttac gtaacgtcca gataccgcca atcagtgcca gtagcagcaa caccacggtg
     2821 gtggcaatca tcgcattcag gcgatattgc ggatcaaccg cccaaataat tagcaccatc
     2881 aggctcataa gaccaaatgc agcgaaaagc atcgtcaggc ccagcatcag taaaagttga
     2941 aagagattag ctttttcctc ttccagctca accaccgcca gccgcagacg tgtttccacc
     3001 atttcaacca tgatggaaac aattcgctgc ccgatgccca gaacgctttt accgggccct
     3061 tgcgcgtgat gagtgtccgc cataattaac gacgcgacag cagaacgccg agcactacac
     3121 cgattgcagc gccaatgccc acgcccgtcc atggattttc acgcacatac tcatcggcac
     3181 gcgccgccgc gacacgggtt tgtttggcga tggcatcacc ggtttcaccc aggcgataac
     3241 ggctctgttt cagtgcctgc tccgctttgc tacgaatctt actcaactct tctttcgact
     3301 tctcgccaga tgagctaagc acctcttcca gcgtatcgga aagggatttc aactcagcac
     3361 gcagatgttc cgtagtgtgt tctttcgaca tagttttctc caggtgagta agtagtgact
     3421 attgtgagtt agtagtcgcg cgcttccagc tttttcagct cttcctgcgc ttctgccagt
     3481 ttccgttcgc gtttggcaat cttatcggca tcgccttttt gcttcgcctc ggctaaatct
     3541 tgctggcgtt ccgccacctc atctttctgc tttgcgattt tcttctgatg atcggcacgc
     3601 agctggctat ctgaacagtt ggcccggact tcactcaggg ctttattcag accgtcaata
     3661 cgattctggt tttggtgttt ttcggcatag ctgatctcct taaggatatt ttgctccttt
     3721 tcctgacaca gggtagtggc ataactaccg gcactaagag caaaaagaga aacagctaaa
     3781 gcgatgcggt atttcattct tgaaccttcc attgtgattc ggacctatta caaaggaacg
     3841 attgtccaga caacgaagcg gtagtgttaa ccaccgcctc attaatcata gacagttacg
     3901 gtgaaatcac caaagtgagt gatctgtttc ggacaacagg aagttatccg aagcgatgag
     3961 agttatcccg tagccgggtc agccactgca tagcctgact gttattgtcc tgctgcgcaa
     4021 tgatgtagcc ttggggcaat gttctgtcga ggaccgcttt tgcagcggcg ctctggtcgc
     4081 tggagtcaaa ggtaattaac agagtgtcgt ttttgggggt gatacttttg aaaggaatgc
     4141 catgagcgtc aaggtgatgc cagattgaaa aaccgtctgg catcgttgtg ccttgatgaa
     4201 cggcacgaat cgccagcgta gactcttgct ggcgaaccgc ggaccaggcc agcaacagcg
     4261 tgccgaccag aagtaaaaca gcgccggacc aggctagctg gcgaagcgac atgcgaggta
     4321 tttgcatccc ttacccccga tttccatatt tctttttcca taatacgacc agagaacctg
     4381 ccaggccaaa caccagcagc accaccggga gcagcatcag gcatgacatc agctggtcct
     4441 cgtactttaa aaataccggc gttttgccga gcatgtaacc cagagttgtc aggatcaata
     4501 cccacagcag accgctcatc cagttgaaaa actgaaagcg cgcgttattc agccctgata
     4561 acccggcaat cgtcggcagc agtgttctga caaacgcaat aaagcgacca attaacagcg
     4621 ccgataaacc gtgtttatga aaaagatggt gtgcgcgttg atgataatgc gcgggtaaat
     4681 gagatagcca gttttgtacg gtgcgggtat tgcccagcca tcgcccctga atatagctga
     4741 cccagcagcc gaggctggcg gcaacggtca gcagcagaat cgtttgcgga tagcccatcg
     4801 cgcctttcgc aatcaacacg ccgaccaata ccagtaaact gtcgcccggt aaaaaggccg
     4861 ccggaagcaa gccgttttca aggaacaaaa ttacaaacaa gacaaaatac aacatgccaa
     4921 tcatcgatgg attggccagg gtttcaaaat cctgcgccca cagggcttgc agcaattggg
     4981 tcaaaagttc cattcattat tcctggtgaa tcggttaacg ccacgctggt tttaaaagat
     5041 gcactaccgc aatgttattg ctgttttggt atgttctgcg ggtttgatac atcaacacag
     5101 cctgaactat tccctgttaa atcgtgagat aagaaagcat tcaaaaacat aaattcaaat
     5161 tgaatctaat tgtaacaaag gtcaacgttg ttcgttatca aaattacatg tccgtggagt
     5221 ctgattttgc tgaatgcagg ggagggtggc gagaggattt acacaggctg acactttata
     5281 cttttcgctt accgacgaga caaacaggcg cgtcgcagcg cctgtcggac aggtatttca
     5341 tttactgcca ctggtggccg tatcgagatg cactaccggg ttttcggcga acagatagcg
     5401 gtcggcattg aactcgaagt cgtcgctggt ttcgttgaat aacatgatct tggtgttttc
     5461 caggtgctgc cacattgcca gcttagcagc atgaggatct ttgcgaatca gcgccttgag
     5521 gatttgatcg tggtcatcac accagttatc gacggtacgg gaatcaatgt gttcgtgcag
     5581 ttttttccag tacgggttat ggctacgctg ggtccacatt ttttcaacga tagccgccag
     5641 ggcggagttc tgcgtcgcca gggctacctg aatatggaac tgcaaatccc actcggaatc
     5701 acggaagcat tgttcgccgc gcgcctgctc ctggatggcc atcaatttca tgatgtcctg
     5761 tttcgttacc tgagttgctg cgaactcggc aatattactt tcgatgagtt ggcgagcctg
     5821 gagtaactca aacggaccgt aattggcgaa ttccatatta ttgtccgcag cctgctgatg
     5881 gcgcggctgg ttggaaacca cgtgaatacc tgaaccttta cgcacttcga catagccttc
     5941 aacttccagc atgatgatgg cttcacgaac taccgtacgg ctgacgttct tttcatcggc
     6001 aataaagcgc tcagcaggca gtttatcacc caccagatag acgccctgtt cgatgcgctc
     6061 tttcaggtca gcggcaagtt gttgatacaa acggcgtggt tcagtgattt ccatatgcgc
     6121 tccaggcatg atacggcaga ctatatttgt tataccactt ttgcgagttt agctctactc
     6181 gcagtgatga aaaagccgcc tgagaaggcg gctttgaagc gaaactaaaa cttacacgaa
     6241 gcgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa tcgccggttt
     6301 gttctgcaat acggtccaga taaccagcgc acccagcagg tcaaatactg ccagaactgc
     6361 gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg caaacagcgt
     6421 acttgccagc catgcggaca tcccggttaa accgttcgcc gttgccactt cgttacgacc
     6481 aaacacgtcg gaagagagcg taatcagcgc gccagacagt gcctggtggg caaaaccacc
     6541 gatacacagc agcataattg cgacatacgg gttggtgaac aggccgatca tacccgggcc
     6601 aatcatcagc actgcaccca gcgttacgac catcttacgg gaaacgatca ggttcacacc
     6661 aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga ggtcagcaaa
     6721 cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt aaactttaaa
     6781 catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcca ggaaacgcgg
     6841 cagcgcgata ccccagaact gacggttacg caggatctga ccaacggaca ttttcttcgc
     6901 cgtactcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt cttcatcggt
     6961 cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg cccagataaa
     7021 gctcaatgca ccggagatga taaatgccat ctgccagctg tgcatcacga ttgcccatac
     7081 caccagcggc ggcgcaatca tcgcaccaat cgaagaacct acgttaaaat agcctactgc
     7141 gatggaacgc tctttcgccg ggaaccattc ggagctggct ttcagacccg ccgggatcat
     7201 cgctgcttcc gcggcaccga ccgcaccacg agcaacagcc aggccacccc agctacctgc
     7261 cagcgcggtt gcaccacaga acacggccca cagcacagca aacattgcat aaccgatttt
     7321 cgtacccagc acatccagta cataacctgc taccggttgc atgaccgtat aagcagcaga
     7381 ataggctgcg atgatatagg aatactgttg ggtggagatg tttaactctt ccatcagagt
     7441 tggcgcagct gccgccacag tgttacgcgt caggtaacca agcacggtgc cgagcgtcac
     7501 cagtgcgatc atataccaac gtaacccttt aattttacgc atgtaaaacc tcatcgtttt
     7561 gttatatccg caggaagcgc ggccatcatc ccgccagagg ctgaagatgt tagttacgac
     7621 cgggtaaccg aaaaaaaacc ggcacgcccc gaacgttgcc atgaaagttt cgtgctaatt
     7681 cggcttccgt accggttaac ccgattcatc taacaaatga atgaatcggc aatttgcatt
     7741 ccgtcgactt atgatttgcg acggcagaaa gataacttgt catacaactt taaaaggtga
     7801 gagccatcac aaatgtgcga atatttgtag ggacattacc tgacgacagc gaggccagta
     7861 ctggcgcggc ctgcagagag atttaccact ttgagagtaa tttttttaac tacgtttatt
     7921 gatctaactc acgaaaatat cttcggactc tggaaattgg tgtgataact ttgtcagcat
     7981 cgcaccataa gcaagctagc tcactcgttg agaggaagac gaaaatgact ccgtttatga
     8041 ctgaagattt cctgttagat accgaatttg cccgccgtct gtatcacgac tacgcaaaag
     8101 accagccgat tttcgattac cattgccatt tgccgccgca gcagattgcg gaagactatc
     8161 gttttaaaaa cctgtatgac atctggctga aaggcgatca ctacaaatgg cgcgctatgc
     8221 gtaccaacgg tgtggccgag cgtctgtgta ccggtgatgc gtctgaccgt gaaaaatttg
     8281 acgcctgggc ggcgactgtt ccgcacacta tcggcaaccc gttataccac tggacgcacc
     8341 tcgaactgcg tcgtccgttt ggtatcactg gcaaattgct ttctccgtca actgccgatg
     8401 aaatctggaa cgaatgtaac gaactgctgg cgcaggataa cttctccgcg cgcggcatca
     8461 tgcagcagat gaacgtgaaa atggtcggca ccaccgatga cccgatcgat tctctggagc
     8521 atcacgcaga gatcgccaaa gacggctctt tcaccatcaa agtgctgccg agctggcgtc
     8581 cggacaaagc cttcaacatc gaacaggcga cctttaacga ctacatggcg aagctgggcg
     8641 aagtttccga taccgacatt cgccgctttg ctgacctgca aactgccttg accaaacgtc
     8701 tggatcactt cgccgctcac ggctgtaaag tgtctgacca tgcgctggac gtggtgatgt
     8761 ttgctgaagc gaacgaagcg gaattggaca gcattctggc gcgccgtctg gctggcgaaa
     8821 ccctgagcga gcacgaagtg gcacagttca aaactgcggt actggtgttc cttggtgccg
     8881 aatatgcacg tcgcggctgg gtacagcagt accacattgg cgcgctgcgt aataacaacc
     8941 tgcgtcagtt caaactgctg gggccggatg taggctttga ctccatcaac gaccgtccga
     9001 tggcagaaga gttgtctaag ctgctgagca agcagaacga agaaaacctg ctgccgaaaa
     9061 ccattctgta ctgcctgaac ccgcgcgata acgaagtgct gggcaccatg agcggtaact
     9121 tccagggcga aggtatgccg ggcaaaatgc agttcggttc cggctggtgg tttaacgatc
     9181 agaaagacgg tatggaacgt cagatgaccc aactggcgca gctcggtttg ctgagccgct
     9241 ttgtcggtat gctgactgac agccgtagct tcctgtcata cacccgtcac gaatacttcc
     9301 gccgcattct gtgccagatg atcggtcgct gggtggaagc gggcgaagca ccggcggaca
     9361 tcaacctgct gggcgagatg gtgaaaaata tttgctttaa caatgcgcgt gactacttcg
     9421 ccattgaact gaactaaggt ctggggttga tatgcaatac atcaagatcc atgcgctgga
     9481 taacgtcgcg gtcgctttag cggatttggc tgaaggcaca gaagtcagtg tcgataacca
     9541 aactgttagg ctgcgccagg atgttgctcg tggacataaa tttgcgttaa cgaatatcgc
     9601 aaaaggggcc aacgtcatca aatatggcct gccgattggt tatgcattgg cggatattgc
     9661 ggcgggggaa cacgttcacg cccacaatac gcgcacgaat ctgagcgatc tggatcagta
     9721 tcgctatcaa cctgattttc aggatctgcc tgcgcaagcg gcagatcgtg aagtgcagat
     9781 ctatcgtcgc gctaacggcg atgtcggggt gcgtaacgag ctgtggatcc tgccaaccgt
     9841 gggctgtgtc aacggcatcg cgcggcagat ccagaaccgt tttctgaaag agaccaacaa
     9901 cgctgaaggt accgacggcg tgttcctctt cagccacacc tacggctgct cacagctggg
     9961 cgacgatcac attaataccc gcaccatgct gcaaaacatg gtgcgccacc cgaacgcagg
    10021 cgcagtgctg gtgattggtc tgggctgtga aaacaaccag gttgccgcat tccgtgaaac
    10081 gctgggcgat atcgatcctg aacgcgttca tttcatgatc tgccaacagc aggatgatga
    10141 gatcgaagcc ggaatcgagc atttgcatca gctgtataac gtgatgcgca acgataaacg
    10201 cgagccaggc aaactcagcg aactgaagtt tggtctggag tgcggtggtt ctgacggtct
    10261 ttctggtatt actgctaacc cgatgctggg gcgtttctct gactacgtga ttgctaacgg
    10321 cggtactacc gtactgaccg aagtgccgga gatgtttggc gcagagcagt tgctgatgga
    10381 ccattgccgc gacgaagcaa cgtttgaaaa actggtcacc atggtcaacg acttcaaaca
    10441 gtactttatt gcccatgacc agccgatcta tgaaaaccca tcgccgggga acaaagcggg
    10501 cggtatcacc acgctggaag ataaatccct tggctgtacc cagaaagcgg gttccagcgt
    10561 cgtggttgac gtgctgcgtt acggcgagcg tctgaaaacg ccggggctga acttgttaag
    10621 tgcgccgggt aacgatgccg tagcgaccag cgccctggcg ggtgcgggct gccatatggt
    10681 gctgttcagt actggtcgtg gcacgccata cggcggcttt gtaccgacgg tgaaaatcgc
    10741 taccaacagt gaactggcgg cgaagaaaaa acactggatc gactttgacg cgggtcagct
    10801 gatccacggt aaagcgatgc cgcagttgct ggaagaattt atcgatacca tcgttgagtt
    10861 tgccaacggt aaacaaacct gcaacgagcg taacgacttc cgcgaactgg cgatcttcaa
    10921 aagcggcgta acgctataaa gtcgaaatga gttgttaata aaaaacggcg tttcataatg
    10981 tgaagcgccg tttgcttttc catacctgat aaggatttat catgaccgcg tattggctgg
    11041 cccagggcgt gggtgtcatc gcctttctga ttggtatcac aacatttttc aatcgtgacg
    11101 aacgtcgctt caaaaagcag ctttcggtct atagcgccgt tattggcgta catttttttc
    11161 ttctgggcac ctatcccgct ggtgccagtg ccatccttaa tgccattcgt acattgatta
    11221 ccttacgcac gcgcagctta tgggtaatgg cgatttttat tgtgctgact ggcggaattg
    11281 gcctcgcgaa gttccatcat cctgtcgaac tattgccggt tatcggtacg attgtcagta
    11341 cctgggcgct gttccgctgt aaagggctca ccatgcgctg cgtgatgtgg ttttcaacgt
    11401 gttgctgggt gattcacaac ttctgggcgg ggtcgatagg cggcacgatg attgagggta
    11461 gttttctgct tatgaatggc ctgaatatca ttcgtttctg gcggatgtag aaaaggggaa
    11521 ttgatccgtt taaagtagag aaaacccctc ccgccataga cgaaaggggt taaacaatta
    11581 attacgcagg gcgctatttg ccagacgatc gtcttccgcc tggcaagctg ccgcagtgaa
    11641 cagcacgtca gttgaagagt tcagcgcggt ttcgcaagag tcctgcaata cgccgataat
    11701 aaagccgacg gcaaccacct gcatggcgat atcgttcgaa ataccgaaca tattacaggc
    11761 cagtgggatc agcagcaaag aaccccccgc cacgccggat gcgccacagg cacacagaga
    11821 agccactacg ctcaacagca gcgctgtggg cagatcgacc ggaataccca gcgtattaac
    11881 cgcagccagc gtcaacacgg taatggtgat tgctgcgccc gccatattga tggtggctcc
    11941 cagcggaata gaaacggaat aggtatcgcg atccagattc agcttttcac acagtgccat
    12001 attcaccgga atgttcgccg cagagctgcg ggtgaagaag gcatacacgc cgctttcgcg
    12061 caggcacagc agcaccagcg ggaacgggtt acgacgaatt ttccaccaca ccagcaatgg
    12121 gttaaccacc agcgccacca gtaacataca gccaaccagc acgaccagca gttgcgcgta
    12181 gccccacagt gtggagaaac cggtggttgc cagggtagaa gaaaccagac caaaaatacc
    12241 aatcggtgcg aagcgaatga ccagtttcac cataaaggtg acggcattcg acatatcgtt
    12301 gaccaggttt ttggtggtct cgttaccgtg acgcagtgcg aaaccgaggc caattgccca
    12361 caccaggatc ccgatgtagt tacctttcag cagcgcgtcg atggggttgg aaaccatgct
    12421 cattaccagc ccgcgcatca cttctacaat gcctgacggt ggcgaaatat cacccgcgct
    12481 actggataag tgcagggtag aagggaaggc gaagctgaaa acgactgcgg ccagagcagc
    12541 agagaaggta cccagcagat agaggaacaa aatagggcgg atattggttt tctgcccgtg
    12601 ctggtggtta gcaatagatg ccatcaccag catcaacacc aggatggggg caacggcttt
    12661 cagtgcgccg acgaacaaag tacctaacag accaacagct tccgccgcgg gttttgagat
    12721 ccatgccaga agaatcccca gaacaaggcc gaccaggatt tgttttacca ggctgccatg
    12781 agccagacgc cggaatagcc ccggtgaacg ttgcgtagtc atttttcgat cctttcattg
    12841 tgttgtcgca catccctggt gcgttctgtc gacgcatctt tcaggatgta acaaagtgtt
    12901 tgcaaggtcg agtataagga aagttacagc ttcgggaagc gaaaaatgct ggattttaaa
    12961 gagaggtcat attttgcaac ttaatggtta catttatttg acataaaaat aaagccaacg
    13021 tgcggcttcc agactgagga ctacgtcaaa aaaggactcg gtcagtcccc tcgccccgtt
    13081 tggggagagg gttagggtga ggggaacagg ccagcatcgg tgcgaaaaat taccctcacc
    13141 ccgaccctct ccctggaagg gagagggaga aaacggtgct catgttgatg acagggtaca
    13201 cggcttaggc agattaaaaa tcatccaacc cgctgcttat catgccgata attcacccag
    13261 gcgttgataa taaacgtcat cactaggatg ccaaacacca cgcccagcga gacggcgatt
    13321 ggaatatggt agaagtcgac aatcagcatc ttgataccga taaacaccag aatcaccgcc
    13381 aggccatatt tgagcatcga gaaacgctct gctacgcccg ccagcaggaa atacatcgca
    13441 cgcaggccga ggatcgcaaa caggtttgag gtcagcacaa tgaacgggtc agtggtcacg
    13501 gcgaagatag ccggaatgct atccacggca aaaatcacgt cgctcaattc caccagaatc
    13561 agtaccagca tcagcggtgt ggcgtacagc aatccattct tacgcacaaa gaaatgctcg
    13621 ttgtcgatgg tgtcggtcat gcgcaaatga ccgcgtagcc agcgcaccag cggcttgtcg
    13681 ccaatgcctg attcatcttc atgggcgagc gccatcttca cgccggtaaa cagcaggaag
    13741 gcaccgaaga tatacaggat ccagtcgaac tgcgaaatca gccagctgcc agtgaagatc
    13801 atgatggtac gcagaacaat cgccccgagc acgccataca ccagcacgcg gcgttgtaat
    13861 gccgccggaa cggagaaata gctgaacaac atcagccaga caaagacgtt atcgaccgcc
    13921 agcgacttct caatcagata acccgtgagg aaggccagcg cctgtggatc ggcaacggcg
    13981 cgaccttcgg tttgtaccag ataccaccag aaagcagcgt taaacagtaa cgacagcgtc
    14041 acccagacca gcgaccaggc cgcagcctgt ttcatggtca tggcatgtgc cccacgacgt
    14101 ccctgcaaca acaggtcgat agccagcata atggcgacaa caacagcgaa tccgccccat
    14161 agcaacggcg tgccgacagt attcatagaa gttccttaca cataaaaaaa acggccaacg
    14221 tcggaagacg tcagccgctg ctttttatgc atagacctcg ccttccggca aggtctcact
    14281 tacaacaaaa aaggaatacg tctgcgtatt cctttcgggt tgcccggtga ccggatgtgg
    14341 tttttcacac atcgtaatga cgatcgaccg gcaatgaagt tactcctctt tgcaggtaac
    14401 aaagtatgac agaccattca gtcagtcaat ggtctgttac atacctttta cactgtttta
    14461 ctttggcaat tatagtttta cgctatctgc cggaaaaatc accccagtct ggcggcggat
    14521 ctcggtcagc agtttggctg tgatgcgact gaccgccagc cctggatgat ccaccagatg
    14581 ctcatccacc agggtagcga acagctctgc ttcgtagagc atggtattaa tatgctgcgg
    14641 ctgggtgaga tcctgcatct ggctgccacg cggcacgaag cacactttct ggcattcaga
    14701 cagtttttca atcaccagcg atcccgcttc gccctgaatc tcgcttgcca ggacagaatc
    14761 gctgaccttt gagtgctgca aggtgacgct gaaatcaccg taatccatca ccaccacgcc
    14821 gtgggcatct acaccacttg ccagtaaact ggcggttgcc tgcacgcttt tcggctcacc
    14881 aaataacgct accgccgacg ccaggcagta aaagccgata tccataattg aaccgttaga
    14941 gaatgccgga ttaaaggtgt tgggattctc accgtccagg taacgttgat accgcgagga
    15001 atactggcaa tagttgaaaa aaactttccg cagtttgccg actttcggca gcgcctggcg
    15061 taacagatga aagttaggca ggctggccgt tttaaatgcc tcaaacagca ccacctgatt
    15121 ttcacgcgcg caggcaatgg ctgcatccac ttccgccaga ttcgacgcca gtggtttctc
    15181 acaaatcaca tgaattttgt ggctcaggaa aagttgcgtc tgggaaaaat gcagggcgtt
    15241 cgggctggca atatacaccg cgtcgatagc gtcactttcc gccattgctt ccagcgaagt
    15301 aaacagatgc tcgacagaaa actcattggc gaagtgctgg gcctgttcaa ggctgcggga
    15361 ataaacggcg gttaacttat atttaccgct ttcatgtgcg gcctccacga actggtgagt
    15421 tatccagttc gtaccaatca cggcgaaacg tatcataacg cgtggatact ccataaaggg
    15481 attctgtcgt caatgtagca cgcctttttc atcttcgacg tgcgcggata cgcattattt
    15541 ctgcgccgga caaccatgct cactaaacgg accaataatg accgacggcc cgagatattt
    15601 tggctgacga ttgaagattt tcgcgtcaat caccgcgctg actctggcga gtttctctcg
    15661 cagacggaca atctttccgc gtcctttttc caggtctggt gcgttaaacc cgatagcatc
    15721 gatgccgtat tgttttgcca gccagatggc gcgttggtta tgaaattcct gcgagataat
    15781 ggtgatatgg ttctccccaa acactttatt ggcacgcacc actgaatcaa gcgttgagaa
    15841 tccggcatag tcacagaaga tcacttttgc cggtacgccc tttgcgatca aagcctgctg
    15901 catgcctgac gcttcatcgt aatttttacg tccgttatcg ccactgacca gcagccattt
    15961 cacttttccg gcgtggtaca actctgctgc ggtatcaata cgtcgggtaa agtagcgatt
    16021 acccggcctg gcccccagca ataagcccac gttacgcgcc ggaacgacgt tgacgtcgct
    16081 ccaggtcagc tgtttgctgg cgttgaccat tacgcgatcg gcgataaaaa tggtcgctcc
    16141 tgccacaagt aacagtaagc aggcaatctt tagagtccgg cgagaaaaac aaatacggag
    16201 aagaaggcgg gcaaatgcgc gcagcatggt cgttatcctt aacgaaaggg gggaggtcga
    16261 ttttaggcgc taaagaaaga aaagataacg cctgacatca ttttcagatt ttgtcctgcc
    16321 caagcgacaa ctgggttagc cacagtcggg tgtcgaactc aagctggtgg tactggggtt
    16381 ccatatgaca gcacaactgg taaaacgctt tgttatgctc tttctccttc aggtgcgcca
    16441 gctcgtgcac cacgatcatg cgcaaaaacg gttccggcgc gttgcgaaac acggtagcga
    16501 cgcggatctc tgcttttgct ttcagcttgc cgccctgcac acgagaaaca gcggtatgta
    16561 acccgagcgc gttttttagc acatggatct tattgtcata catcacttta ttgatcggcg
    16621 gggcattacg cagaaactga tttttcagat cctgagtata ttgccagagg gctttatcgg
    16681 tggcgtagtc gtgtgttccc ggatagcgtt ttgccagcac atcgcccaga cgctgttcgt
    16741 taatcagtgt tcgcacctgg gaaagtagct gctcgggata accctgaaga taagtaagat
    16801 tgctcataac cgcccacgaa aaagagaaaa agggtatact cacgcaccct tttcaggggc
    16861 aatgtcgaaa ttttatcatt caggagggcc gatgagccac ttagacaacg gtttccgttc
    16921 actgacacta caacgttttc cggcgacgga tgacgttaac ccgctacagg cgtgggaagc
    16981 ggcggatgaa tatttgctgc aacagttgga cgacacagaa atccgcggcc cggtgttgat
    17041 cctgaatgat gcctttggtg cgttaagctg cgcgctggcg gaacataagc cgtacagcat
    17101 tggcgactca tacatcagtg aactggcgac gcgcgagaat ttacgcctca acgggattga
    17161 tgagtcgagc gtgaagtttc tcgacagcac cgccgactac ccgcaacagc cgggtgtggt
    17221 gctgatcaaa gtgccgaaaa cactggcatt gctggaacag caactgcgtg cgctgcgcaa
    17281 agtggtcacg ccacaaacac gtattattgc cggtgccaaa gcccgtgaca tccacacttc
    17341 cacgctggaa ctattcgaaa aagtgctcgg cccgaccacc accacgctgg catggaagaa
    17401 agcgcgcctg attaactgca ctttcaatga accaccgctg gccgatgcgc cgcagaccgt
    17461 tagctggaag ctggaaggta ctgactggac tatccacaac catgcgaatg tcttctcccg
    17521 caccgggctg gatatcggcg cgcgcttctt tatgcaacat ctgccagaga atctcgaagg
    17581 ggagattgtc gatctcggtt gcggtaatgg cgttattggt ctgacgctgc ttgataaaaa
    17641 cccgcaggcg aaagtggtgt ttgtcgatga atcgccgatg gcggttgctt ccagccgttt
    17701 gaacgttgaa accaacatgc cagaggcgtt ggatcgctgc gaatttatga tcaacaacgc
    17761 gctctccggc gtggagcctt tccgctttaa tgctgtgctc tgcaacccgc cgtttcatca
    17821 gcaacatgcg ctgaccgata acgtcgcctg ggagatgttc caccacgccc gccgctgcct
    17881 gaaaatcaac ggcgagctgt atatcgttgc caaccgtcac ctggattact tccataaact
    17941 gaagaaaatt ttcggcaact gcaccaccat cgccacgaat aataaatttg tggtgctgaa
    18001 agcagtgaag ctggggcgtc gtcggtaaga ttgacagcta cctgccagat aaggcgtaaa
    18061 cgccttatct ggcatacgtt tagcacatta cccccaggtc ggataagatg cggcaagcgt
    18121 cgcatccggc aatgtctgcc cagttctgga atcgcgctcc ataaaacaaa aaatccctgt
    18181 caccatcctc tacattctct tgtttagcgt tgtttaacgt ttattcttcc gtcacacaga
    18241 taaattcatc cgttgccaat ctgtcaacgg atgttatcat attgccaatt tgtcaacgga
    18301 cgtgatgaag aatgcacctg ataactcaaa aagcattgaa agatgctgcg gaaaaatacc
    18361 cgcaacataa aacggagttg gtggctctag ggaacacgat tgctaaggga tatttcaaaa
    18421 aacctgagtc attaaaagca gtattcccat ctctggataa cttcaaatat ctggataagc
    18481 attatgtttt caatgttggg ggcaatgaat tacgtgttgt agcaatggtc ttttttgaat
    18541 cgcaaaagtg ctacatacgt gaagttatga cgcataaaga atacgatttt tttaccgctg
    18601 ttcatcgtac taaggggaaa aaatgattgc gattgccgac atcttgcatg caggagaaaa
    18661 gctaactgct gtggcacctt ttctggcggg tattcagaac gaggaacaat acatccaggc
    18721 gctggaactg gtagatcatc tgctgctcaa cgatcctgaa aaccccttac tggatctggt
    18781 gtgtgccaaa ataaccgcgt gggaagaatc agcgcccgaa tttgcggaat ttaatgccat
    18841 ggctcaagcc atgcctggcg gtatagccgt gattcgtacc cttatggatc aatatggttt
    18901 aaccctttcc gatctgccgg aaattggcag taaatctatg gtgtcacgcg ttttgagcgg
    18961 gaagaggaaa ttaacgctgg aacacgctaa aaaattggca acgcgattcg gcatttctcc
    19021 cgccttgttt attgattaac gcg
//
LOCUS       DAZFBR010000092        18802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFBR010000092 DAZFBR010000000
VERSION     DAZFBR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.092"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            218..1789
                     /gene="garD"
                     /locus_tag="ACHJZ2_003519"
     CDS             218..1789
                     /gene="garD"
                     /locus_tag="ACHJZ2_003519"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO4569564.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1938..2273
                     /gene="prlF"
                     /locus_tag="ACHJZ2_003520"
     CDS             1938..2273
                     /gene="prlF"
                     /locus_tag="ACHJZ2_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO4569565.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2273..2737
                     /gene="yhaV"
                     /locus_tag="ACHJZ2_003521"
     CDS             2273..2737
                     /gene="yhaV"
                     /locus_tag="ACHJZ2_003521"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO4569566.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2792..3601)
                     /gene="agaR"
                     /locus_tag="ACHJZ2_003522"
     CDS             complement(2792..3601)
                     /gene="agaR"
                     /locus_tag="ACHJZ2_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO4569567.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3850..5130
                     /gene="kbaZ"
                     /locus_tag="ACHJZ2_003523"
     CDS             3850..5130
                     /gene="kbaZ"
                     /locus_tag="ACHJZ2_003523"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO4569568.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5153..5626
                     /gene="agaV"
                     /locus_tag="ACHJZ2_003524"
     CDS             5153..5626
                     /gene="agaV"
                     /locus_tag="ACHJZ2_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO4569569.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            5637..6416
                     /gene="agaW"
                     /locus_tag="ACHJZ2_003525"
     CDS             5637..6416
                     /gene="agaW"
                     /locus_tag="ACHJZ2_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO4569570.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6406..7284
                     /gene="agaE"
                     /locus_tag="ACHJZ2_003526"
     CDS             6406..7284
                     /gene="agaE"
                     /locus_tag="ACHJZ2_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO4569571.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7302..7736
                     /gene="agaF"
                     /locus_tag="ACHJZ2_003527"
     CDS             7302..7736
                     /gene="agaF"
                     /locus_tag="ACHJZ2_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO4569572.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7733..8866
                     /gene="nagA"
                     /locus_tag="ACHJZ2_003528"
     CDS             7733..8866
                     /gene="nagA"
                     /locus_tag="ACHJZ2_003528"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO4569573.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9217..10371
                     /locus_tag="ACHJZ2_003529"
     CDS             9217..10371
                     /locus_tag="ACHJZ2_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO4569574.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10384..11244
                     /gene="kbaY"
                     /locus_tag="ACHJZ2_003530"
     CDS             10384..11244
                     /gene="kbaY"
                     /locus_tag="ACHJZ2_003530"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO4569575.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11411..11887
                     /gene="agaB"
                     /locus_tag="ACHJZ2_003531"
     CDS             11411..11887
                     /gene="agaB"
                     /locus_tag="ACHJZ2_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO4569576.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGGVSFRSGD"
     gene            11835..12626
                     /gene="agaC"
                     /locus_tag="ACHJZ2_003532"
     CDS             11835..12626
                     /gene="agaC"
                     /locus_tag="ACHJZ2_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO4569577.1"
                     /translation="MFSFRTSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDIQ
                     TGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLL
                     MQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQ
                     GAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQV
                     SNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12616..13407
                     /gene="agaD"
                     /locus_tag="ACHJZ2_003533"
     CDS             12616..13407
                     /gene="agaD"
                     /locus_tag="ACHJZ2_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO4569578.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13408..14163
                     /locus_tag="ACHJZ2_003534"
                     /pseudo
     CDS             13408..14163
                     /locus_tag="ACHJZ2_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
     gene            14564..15148
                     /locus_tag="ACHJZ2_003535"
     CDS             14564..15148
                     /locus_tag="ACHJZ2_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4569579.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            15228..15923
                     /locus_tag="ACHJZ2_003536"
     CDS             15228..15923
                     /locus_tag="ACHJZ2_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4569580.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            15952..18468
                     /locus_tag="ACHJZ2_003537"
     CDS             15952..18468
                     /locus_tag="ACHJZ2_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4569581.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            18479..>18802
                     /locus_tag="ACHJZ2_003538"
     CDS             18479..>18802
                     /locus_tag="ACHJZ2_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4569582.1"
                     /translation="MKRAPLITGLLLISTSCAYASSEGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKT"
BASE COUNT         4759 a         4644 c         4737 g         4662 t
ORIGIN      
        1 taaatattat tttgagcata tgcacataag gttgcgcgct aaagcacaga tttgcgcttt
       61 accttgccgg gcggcattgc aatccctgaa atgattgaca ttgatcacat ttctgcgttt
      121 aaactcctga cattcttatt tcacccaatg aagtcattta tttttaaatg agaccaggtc
      181 ctcattttaa taacctctgg ctggagaata ttgcacaatg gccaacatcg aaatcagaca
      241 agaaacgcca actgcgtttt atataaaagt tcacgacaca gataatgtgg caattattgt
      301 taatgataat ggcctgaaag caggaacgcg ttttccggat gggctggaat taattgaaca
      361 tattccccag gggcataaag tcgcattgct cgacattccg gctaatggtg aaattattcg
      421 ttatggcgaa gtgattggtt acgccgtgcg tgcaatccca cgcggaagct ggatcgacga
      481 atcaatggtt gtactgccga aagcgccgcc gttacacacg ctgccactgg caaccaaagt
      541 cccggaaccc ttaccgccgc tggaaggata cacctttgag ggctatcgca atgccgatgg
      601 cagcgtgggc accaaaaacc tgctcggcat caccaccagc gtccactgtg tggcaggcgt
      661 ggtggactac gtagtaaaaa tcattgaacg cgatctgcta ccgaaatacc cgaacgtcga
      721 tggcgtggtg gggctgaatc atttgtacgg ttgtggcgtg gcgattaacg caccggcggc
      781 agttgtacct atccgtacca ttcacaatat ttcgctgaat cctaactttg gcggcgaagt
      841 aatggtgatt ggcctgggtt gtgaaaagtt gcagcctgag cgcctgctga ctggaacgga
      901 tgatgtgcaa gctattccag tagaaagcgc cagcattgtc agtttgcagg atgaaaagca
      961 tgtcggtttt cagtccatgg tcgaggatat tttgcaggtc gccgaacgcc atctacaaaa
     1021 actgaatcaa cgtcagcgag aaacctgtcc ggcttcagaa ctggttgtcg gcatgcagtg
     1081 cggtggcagc gatgcctttt ctggcgtaac ggcaaacccg gcggttgggt atgcgtctga
     1141 tctactggtg cgctgcggcg caacggtgat gttctcagaa gtcacggaag tgcgtgacgc
     1201 gatccatctg ctgacaccac gcgcagtgaa cgaagaggtc ggcaaacggc tgctggagga
     1261 gatggagtgg tacgataact atctcaatat gggaaaaacc gaccgcagcg ccaacccttc
     1321 gccgggcaac aagaaaggcg gtctggcaaa tgtagtggag aaagcgctcg gctccattgc
     1381 taaatcgggt aaaagtgcaa ttgttgaagt gctgtcgccc ggtcaacgcc cgactaaacg
     1441 cggattaatt tacgccgcga cgccagccag cgattttgtc tgtggcacgc aacaggtggc
     1501 ttcgggtatc accgtgcaag tgtttacgac cggtcgtggt acgccgtacg gcctgatggc
     1561 ggtacccgtc attaaaatgg cgacccgcac cgagctggcg aaccgctggt ttgatttaat
     1621 ggatattaac gcgggcacta tcgccaccgg cgaagaaacc attgaagagg tgggctggaa
     1681 gttgttccac tttattctcg acgtcgccag cgggaagaag aaaaccttct cggatcaatg
     1741 gggactgcat aaccagctgg cggtgtttaa cccagcaccg gtgacctgat ttcttttgga
     1801 acgtccctcg caaaacatgg ccttagtgcc atgtttttat tgtttaaagc cctcacgtcc
     1861 attaataatg catttgcatt accttagttc aagcttataa tttgagcaga aaacaggctg
     1921 taaaaggaca gtgaatcatg cccgctaatg ctcgctctca cgctgtactg accactgaat
     1981 caaaggtcac gatacgcgga caaacaacta tccccgcgcc agtgcgtgag gccttaaaac
     2041 tgaagccagg ccaggacagc attcattacg aaattctgcc tggtgggcaa gtatttatgt
     2101 gccgactggg agatgagcag gaggatcata ctatgaatgc atttttgcgt tttctggata
     2161 cagatatcca gaacaacccg caaaaaactc gtccattcaa cattcaacaa ggaaagaaac
     2221 ttgtcgctgg catggacgtc aacattgatg atgagattgg cgacgacgaa taatggattt
     2281 tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg aaacctacga
     2341 cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt atcagagaaa
     2401 agctgccaca aagttattgg cggtggtcca taaagtgatt gaggagcata tcacggtcaa
     2461 tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa ataaagactg
     2521 gtcacgggta aaatttggtg ctggtcgcta tcgtctcttc tttcgttaca gtgaaaaaga
     2581 gaaagtcatc attctgggat ggatgaacga tgaaaacacg ctgcgcacct acggtaaaaa
     2641 aacagatgcc tataccgtat tcagcaaaat gttaaaaaga ggacatcctc ctgccgactg
     2701 ggaaaccctc acccgagaga cagaagaaac ccattgatgg tgttcacaat gcccgccgaa
     2761 cgcattcgtg ttgaccagcg ggcacccttt ctcactcccc gaccagaatc acttcaaccc
     2821 cagcctttcg cagcccttcc aggctatccg caggaatgcc ttcatcaaca atgatcatgt
     2881 cgatacgttg agtatcaatg atcttatgta aactggaacg attgaattta ctggaatcgg
     2941 tgaccacgat gatccgttcc gcaacttcgc acatccggcg gtttaaacgg gcttcatctt
     3001 cattatgcgt gctgacgccg cgctccagat cgatcgcatc tacaccaaga aacagcatat
     3061 cgaagtggta attttgcagc gattgctcag cctgatcgcc gtaaaaagat tgcgactgac
     3121 ggcgcaaatg cccgccggtc atcagcagct caacgccttc cgcttccagc aacgcattag
     3181 ccacgttcat accgttggtc atcgcaatta cgtcagtgtg cttgcgcatc agacgagcaa
     3241 tctcaaaagt ggtggtcccg gaatcgagga tcacccgatg acctggctga atcaactcaa
     3301 cggcagcttt cgcaacgctg cgtttcattg cggtgttcag cgcgctttta tcttccactg
     3361 atggctcgac tgacggcgtt gtgctatcgc agatcaacgc gccaccatag gcacgcacag
     3421 cgatcccctg cttttccaga aacgccagat cgttgcggat cgtcacggta gatacgccat
     3481 acaatgccga cagatcgtta acctgcacac tcccttgctg tcgcagacgc tgaatgatct
     3541 gttctcgtcg ctcgctggtg cctgtcactc gcttctcacc tgaagcgtcg gtattactca
     3601 tagtaagtcc tttcgtaaaa ctttcgtttc atttcgtttt gcctattaac gcctttctat
     3661 taagcaaatg caagcccacc ttgcccattg acgcaagcta ctctcgtttc agtgactttc
     3721 attatgtttc ttttgtgaat cagatcagaa aactattatc tttcgtttta tttttatctc
     3781 accatgacgc agtatcaact gaaacaaaac gaaagattaa tatcgcagta atctgaactg
     3841 gagaggaaag tgaaacatct gacagaaatg gtgagacagc acaaagcggg caaaacaaat
     3901 ggaatttatg ccgtttgttc cgcacatccg ctggtgctgg aggctgcaat ccgctacgcc
     3961 agtgcaaacc aaacgccgtt actgattgaa gcaacctcca atcaggtaga ccagttcggc
     4021 ggttataccg gaatgacgcc cgccgatttt cgcggctttg tttgtcagct cgccgactcg
     4081 ttgaatttcc cgcaggatgc gttgattctg ggtggtgacc atctggggcc aaaccgctgg
     4141 caaaacctgc cggccgctca ggcaatggcc aatgccgatg atttgattaa aagctacgtt
     4201 gcggcaggat tcaaaaaaat ccaccttgat tgcagcatgt cctgtcagga cgatccgatt
     4261 cccttaactg atgacatcgt ggctgaacgc gccgcccgtc tggcgaaagt ggcggaagaa
     4321 acctgtcttg aacactttgg cgaagccgat ctggagtatg tcattggtac cgaagtgccg
     4381 gtacctggcg gcgcgcatga aaccttaagc gagctggcgg tcaccacgcc ggatgccgcc
     4441 cgcgccacgc tggaagccca tcgtcacgcc tttgaaaagc aaggtttgaa tgccatctgg
     4501 ccacgcatca ttgccctggt ggttcaaccc ggcgtcgaat tcgatcacac caacgttatt
     4561 gattatcagc ccgccaaagc gaccgcctta agccagatgg tcgaaagcta cgaaacgctg
     4621 attttcgaag cgcactctac cgattatcaa acgccgcaat cgctgcgcca gctggtgatt
     4681 gaccactttg ccattctgaa agttggcccg gcgctgacct tcgccctgcg tgaagctctg
     4741 ttctctctgg cagcgattga agaagaactg gtgccagcaa aagcctgttc tggtctgcgt
     4801 caggtgctgg aaaacgtgat gctcaaccgc ccggaatact ggcaaagcca ctaccacggt
     4861 gacggcaacg cgcgtcgtct ggcgcgtggt tatagctact cggatcgcgt gcgctattac
     4921 tggccggaca gccagattga tgacgctttc gctcatctgg tacgtaatct ggcggattca
     4981 ccaattccgc tgccgctgat cagccagtat ctgccgctgc agtacgtgaa agttcgctcc
     5041 ggcgagctgc agccaacgcc acgggaactc attatcaacc atattcagga catcctggcg
     5101 cagtaccaca cagcctgtga aggccaataa gcaaaacaaa gaggaacacg ctatgccaaa
     5161 tattgtttta agccggattg atgaacgctt gattcacggt caggtcggcg ttcagtgggt
     5221 cggatttgcg ggggcaaatc tggtgctggt cgccaacgat gaggttgccg aagatccggt
     5281 acaacaaaac ctgatggaaa tggtactggc agaagggatc gccgtacgtt tctggacgct
     5341 gcaaaaagtt atcgacaaca ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg
     5401 taaaacaccc gccgatttcc tgacgctggt gaaaggtggc gttccggtga atcgcattaa
     5461 cgttggcaat atgcactacg ccaatggcaa gcaacaaatc gccaaaacgg tttctgtgga
     5521 tgcgggcgat atcgcagcat ttaacgacct gaaagccgct ggggtggaat gcttcgttca
     5581 gggcatcccg acagagcctg ctgtggacct ctttaaatta ctttgaggga ttcatcatgg
     5641 aaatcagtct gttgcaggca ttcgcgttgg gcattatcgc ctttatcgct ggcctggata
     5701 tgtttaacgg cttaacccat atgcaccgcc cagtggtcct cggcccgtta gtcgggctgg
     5761 tacttggcga tctgcatacc ggtattttaa ccggcggtac gctggaactg gtgtggatgg
     5821 ggctggcccc gctggcgggc gcacagccgc ctaatgtgat tatcggtact atcgtcggca
     5881 cggcgtttgc cattactact ggcgtgaaac ccgatgtcgc agtcggtgtc gccgtacctt
     5941 tcgctgtcgc agtacagatg gggattacct tcctgttctc ggtgatgtct ggcgtgatgt
     6001 ctcgctgcga ccggatggcg gagaacgccg atactcgcgg cattgaacgc gtaaactatc
     6061 tggcactgct ggcactcggc actttctatt ttctctgcgc tttcctgcct atctacttcg
     6121 gcgcggaaca tgcaaaaacc atcattgatg tcctgccgca acgattaatc gacggcctcg
     6181 gcgtcgcggg cggcatcatg ccagcaatcg gttttgccgt gctgctgaaa atcatgatga
     6241 aaaacgtcta catcccctac ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac
     6301 cggtgctggc tattgctgcc gccgcgctgg cgatggcgct gatcgatctg ctgcgtaaat
     6361 ctcctgaacc gactcaacct gcggcacaga aagaggaatt tgaagatggc atctaatcaa
     6421 accaccctgc cgaacgtcag tgaaaacgaa gaaacattgc tgactggcgt caatgaaaac
     6481 gtgtatgaag atcagagcat tggcgcggag ctgacgaaaa aagatatcaa tcgcgtcgcc
     6541 tggcgttcca tgctgttaca agcgtctttt aactacgaac gtatgcaggc ttccggctgg
     6601 ttgtacggtc tgctgcctgc tctgaaaaag atccacacta ataaacgcga cctggcgcgc
     6661 gccatgaagg ggcatatggg tttcttcaat acccatccgt ttctggtgac atttgttatc
     6721 ggcattatcc ttgcgatgga gcgttctaag caggacgtta acagtattca gagcaccaaa
     6781 attgccgtcg gtgcgccgct cggcgggatt ggcgatgcaa tgttctggct aacgctactg
     6841 ccgatttgtg gcgggattgg agccagtctg gcattgcaag gctccattct tggcgcagtc
     6901 gtctttattg tgctgttcaa cgtggtccac ctggggctgc gttttggtct ggcgcattat
     6961 gcttaccgca tgggcgtggc ggcgattcca ctcattaaag ctaataccaa aaaagtcggc
     7021 catgctgcgt ctatcgttgg gatgacggta atcggtgcgc tggtggcaac ctatgttcgt
     7081 ttaagcacca cgctggaaat cacagcgggc gacgcagtgg ttaagttaca ggctgatgtt
     7141 atcgacaaac tgatgcctgc cttcttaccg ttggtctaca ccctgaccat gttttggttg
     7201 gtacgccgcg gctggagtcc gctgcgtctg attgcagtta ccgtggttct cggcatcgtt
     7261 ggtaaattct gccatttcct ttaaatacaa tgaggtatta gatgttaagt attattttga
     7321 cagggcatgg cgggtttgcc agcggcatgg aaaaagcaat gaagcaaatt ctcggtgagc
     7381 aatcgcagtt tatcgccatc gattttccgg aaacctcaag caccgcgctg cttacatcgc
     7441 agcttgaaga ggccatcgcc caactggatt gtgaagacgg cattgttttt ctgacggatt
     7501 tgctgggcgg cacgccgttt cgcgtagctt caacgctggc gatgcaaaaa ccgggctgtg
     7561 aagtgatcac cggcaccaat ttgcaactgc tactggagat ggtgctggag cgcgaagggt
     7621 taagcggtga agaattccgt gtacaggcgc tggagtgtgg acatcgtggg ctgaccagtc
     7681 tggtggacga gttaggccgc tgccatgaag aatgtccggt cgaggaaggg atatgacaca
     7741 cgttctgcgc gccagaaggc tgctgactga agagggatgg ctcgatgacc atcagttgcg
     7801 tattgctgac ggtgtcatcg cagcaatcga accgattcca gtgagcgtga ctgaacgcga
     7861 tgcggaactg ctctgccccg cttacatcga cacccatgta cacggtggtg cgggcgttga
     7921 tgtaatggat gacgcgccgg atgtactcga caagctggca atgcacaagg cacgcgaagg
     7981 tgtcggcagt tggttgccga ctaccgtaac cgcgccgctt agtaccattc atgcggcgct
     8041 gaaacgtatt gctcaacgtt gccaacgcgg cggacctggt gcgcaagtgc tggggagtta
     8101 tctcgaagga ccgtacttca cgccgcagaa taaaggcgcg catccgccgg agttgtttcg
     8161 cgagcttgaa attgccgagc tggatcaatt gattgccgtt tctcagcaca ccttacgcgt
     8221 ggtagcgctg gcaccggaaa aagagggggc attgcaggcc atccgccatc ttaaacagca
     8281 aaatgtacga gtgatgctgg ggcatagcgc ggcgacctgg caacaaactc gcgccgcgtt
     8341 tgatgctggt gccgacggcc tggtgcattg ctataacggg atgacaggtt tacatcaccg
     8401 cgaaccggga atggttggcg cgggattaac ggacaagcgc gcctggctgg aactgatagc
     8461 cgatggtcat catgtgcatc cggcggcaat gtcgctgtgt tgttgctgtg cgaaagagag
     8521 aatcgtactg atcaccgacg cgatgcaggc agccgggatg ccggatggtc gctatacgtt
     8581 atgtggcgaa gaagtgcaga tgcacggtgg cgttgtccgt accgcgtccg gtgggctggc
     8641 gggcagtacg ctgtctgttg atgcggcagt gcgcaacatg gtcgagttga cgggcgtaac
     8701 gcctgcggaa gccattcata tggcgtcgct gcatccggcg cgaatgctgg gtgttgatgg
     8761 tgttctggga tcgcttaaac cgggcaaacg cgccagcgtc gttgcgctgg atagcgggct
     8821 gcatgtgcaa caaatctgga ttcagggtca attagcttcg ttttgatggt ttgctccatt
     8881 attgggcctt cacttccccc gtaaggcctt tctttttctt tcgttttgat ctgtgcagcg
     8941 gtgtcggatg cgacgctaac gcgtcttatc cgacctacag ttggtgaccg caaggccgga
     9001 taaagcgttt gcgccgcatc cagcaatccc ttttgcttcc tttatctttt ctttcaacga
     9061 tcacggattt cgttttattt cctttctctc cattgaactt tcggtttctt ttctatagat
     9121 tttaatcaac gaaagacatc accaagtgaa atgaaacgaa aggcaagtga aagcgacaac
     9181 gcccgacgtc aagttcatca gactaaggat tgagttatgc cagaaaatta cacccctgct
     9241 gccgccgcaa ccggtacatg gactgaagaa gagatccgcc atcagcctcg cgcatggatc
     9301 cgttcgctca ccaacatcga cgcgctacat tccgcgctta ataacttcct tgaaccgtta
     9361 ctgcgcaaag agaatctgcg gatcatcctg accggagccg gaacctcggc atttatcggt
     9421 gacatcatcg cgccgtggct cgccagccat accggtaaaa acttcagcgc cgtaccgacc
     9481 accgatctgg tcactaatcc gatggactac ctgaacccag cccatccgct gctgttgatc
     9541 tccttcggtc gatccggcaa cagcccggaa agcgtcgcag ccgtggaact ggcaaatcaa
     9601 tttgtaccgg aatgctatca cctgccgatc acctgcaacg aagcgggcgc tctttaccaa
     9661 aacgcgatca acagcgataa cgcgtttgcc gtgctgatgc ccgcagaaac gcacgatcgc
     9721 ggctttgcga tgaccagcag cattaccacc atgatggcca gctgcctcgc ggttttcgca
     9781 cctgagacga tcaacagcca aaccttccgc gacgtggcgg atcgttgcca ggcgatcctg
     9841 acctcactgg gcgatttcag cgaaggtgtg tttggttacg caccgtggaa acggatcgtt
     9901 tatctcggca gcggtggctt acagggcgca gcacgcgagt cggcgctgaa agtgctggaa
     9961 ctgacggcgg gtaaactggc ggccttttat gattctccaa ccggattccg tcatggacca
    10021 aaatcgctgg tcgataacga aacactggtg gtggtatttg tctccagcca cccttacacc
    10081 cgtcagtatg atcttgatct gctggctgaa cttcaccgtg acaaccaggc aatgcgtgta
    10141 atcgccatcg ccgcggaaag cagcgacatc gtcgctgccg gtccacatat catcctgcca
    10201 ccgtcacgtc actttatcga cgttgagcag gcattttgct tcctgatgta cgcccagacg
    10261 tttgcactga tgcagtcgct gcacatgggc aatacgccgg ataccccatc agccagtggc
    10321 accgttaacc gcgtggtgca aggcgtaatc attcatccgt ggcaggcata agaggatcgc
    10381 attatgagca ttatctccac caaatatctg ttacaggacg cccaggccaa tggctacgcg
    10441 gtgcctgctt ttaacattca taacgccgag acgatccagg cgatcctcga agtgtgcagt
    10501 gaaatgcgat cgccggtgat cctcgccgga acgccgggga cctttaaaca tatcgcgctg
    10561 gaagagatct acgccctgtg cagcgcctat tccacaacct acaacatgcc actggcgctg
    10621 catctcgacc accacgaatc gctggatgat attcgccgta aagtccacgc aggtgtgcgc
    10681 agtgcgatga tcgacggcag ccacttcccg tttgccgaga acgtaaagct ggtgaaatcg
    10741 gttgtcgact tctgccactc ccaggattgc agcgtggaag cagaactggg ccgcctgggc
    10801 ggtgttgaag atgacatgag cgttgacgcc gaaagtgcat tcctgaccga tccacaagaa
    10861 gctaaacgct ttgtcgaact gactggcgtc gacagcttgg cggtagcgat tggtacggcg
    10921 cacggcttat acagcaaaac gccgaagatt gatttccagc ggctggcgga aattcgtgaa
    10981 gtggtggatg ttcctctggt gctacatggt gccagcgatg ttccggatga atttgtccgt
    11041 cgcactattg aacttggcgt cacaaaagtg aacgttgcca cagaattaaa aatagccttc
    11101 gccggcgcgg ttaaagcctg gtttgcggaa aatccgcagg gtaatgatcc tcgttattat
    11161 atgcgcgtcg gaatggatgc gatgaaagaa gttgtcagaa ataaaattaa tgtctgtggt
    11221 tcagcgaatc gaatttcagc ataatcatta gttttttatg atttatccca atgtacttcc
    11281 cgattaatat cggggagtgc cttaatggaa aaggagatga ctaaacctta ataaatagat
    11341 cagtacaata tcgcaacaat aatatattta aaaaaattat attattcaac tttatggtga
    11401 ggattacaca atgagcagtc caaatattct cttaacccgt attgataacc gtctggttca
    11461 cggtcaggtt ggcgtgacct ggacatccac catcggtgcg aatctgctgg tagtcgtgga
    11521 tgatgttgtc gctaacgatg atattcaaca gaaattaatg ggtattaccg cagaaaccta
    11581 cggctttggc attcgtttct ttactatcga aaaaaccatt aacgtcatcg gcaaagctgc
    11641 accgcatcag aagatcttcc tgatttgccg tacgccacaa acggtacgta aattggtaga
    11701 aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg aagggaaaaa
    11761 gcaaatcagc agtaaagttt atgtcgatga ccaggatctc acggacttac gttttattaa
    11821 acaacgtggc gtgaatgttt tcattcagga cgtccctggc ggcgttagtt ttcgttctgg
    11881 ggattgattt ttggctggaa gccttatttt tattccgccc gataatcgtt tgtaccctaa
    11941 ctggcgctat tctcggtgat attcagactg gcttaattac cggtggtctg acagagttgg
    12001 ctttcgccgg attaacccct gcaggtggtg ttcagccgcc caacccgatt atggcgggtc
    12061 tgatgaccac cgtcattgca tggtctacgg gcgttgatgc caaaacggca attggtcttg
    12121 gcctgccgtt tagtttgtta atgcagtacg tcattctgtt cttctattct gctttctcat
    12181 tatttatgac caaagccgat aaatgcgcga aagaggcgga tacggcagcg ttttcccgac
    12241 ttaactggac aacgatgctc atcgtcgctt cagcgtatgc ggtgattgct ttcctctgta
    12301 cttacctggc acaaggggcg atgcaggcgc tggtgaaagc gatgcccgct tggctgaccc
    12361 acggctttga agtggctggc ggtattctgc ctgccgttgg ttttggcttg ctgctgcgcg
    12421 tgatgtttaa agcacaatat atcccttacc tgatcgccgg cttcctgttt gtttgctaca
    12481 tccaggtcag caacctgttg ccggttgccg tactgggcgc aggctttgcg gtgtatgagt
    12541 ttttcaatgc gaaatcccgg cagcaagcgc aaccgcagcc cgttgccagt aaaaatgaag
    12601 aagaggacta cagcaatggg atctgaaatc agtaaaaaag atatcacccg tctgggcttt
    12661 cgttcgtcgc tgctgcaagc gagctttaac tacgaaagga tgcaagcggg cggttttacc
    12721 tgggcgatgt tgccgatcct gaaaaagatt tataaggatg acaaaccggg cctaagcgca
    12781 gcgatgaaag ataacctcga atttattaat acccacccga atctggtcgg attcctgatg
    12841 gggttattaa tttcgatgga agaaaaagga gaaaaccgcg acaccattaa aggcctcaaa
    12901 gtggcactgt ttggcccaat cgccgggatt ggcgatgcga ttttctggtt tactttgttg
    12961 ccgattatgg cgggaatttg ctcatcattt gccagccagg gaaacctgct ggggccgatt
    13021 ctatttttcg ccgtttacct gcttatcttt ttcctgcgcg tcggctggac ccacgtcggt
    13081 tattcagtcg gcgtgaaggc gatcgataaa gtgcgagaga actcgcagat gattgctcgc
    13141 tcggcaacca tcctcgggat cacggtaatc ggcgggctga tcgcttcgta tgtgcatatt
    13201 aacgtggtga catcgtttgc catcgacagt acccacagcg tcgcgctgca acaggatttc
    13261 ttcgataaag tcttcccgaa cattttaccg atggcctaca ccctgctgat gtattacttc
    13321 ctgcgggtga aaaaagcgca tccggtgctg ttaatcggcg tgacttttgt gctctctatt
    13381 gtttgttccg cattcggcat tttgtaaatg gaacgaggca ctgcgtctgg cggtgcctct
    13441 ttacttaagg aatttcatcc tgtgcaaacc cttcagcaag ttgaaaacta tacggcgtta
    13501 agtgaacgtg ccagcgaata tttattggcc gtgatccgta gcaaaccgga tgccgtgatt
    13561 tgcctggcga ccggagccac gccattactg acgtatcatt atctggtaga aaaaatccac
    13621 tagcagcagg ttgatgtcag ccagctcacc ttcgtgaagc tcgacgaatg ggtggatctg
    13681 ccattaacga tgcctggtac ctgcgaaact ttcctgcaac agcatatcgt gcagccgctg
    13741 gggctacgtg aagaccagct catcagcttt cgctccgaag agataaatga gacagagtgc
    13801 gaacgggtaa cgaacctgat tgcgcgcaaa ggcggtctgg atttatgcgt tctcggattg
    13861 gggaaaaacg gtcatcttgg gctgaacgaa ccgggagaaa acctgcaacc ggcctgccat
    13921 atcagtcaac ttgatgccag aacacagcaa catgagatgt taaaaaccgc gggtcgcccc
    13981 gtgactcgtg ggatcacctt aggcctgaag gatattctca atgcccgcga ggttttgtta
    14041 ctggtgactg gcgaaggaaa gcaggatgcg acagaacgtt ttctcacggc taaagtctct
    14101 acctctatcc cggcttcgtt tttgtggctg cacagtaact ttatttgttt aattaatacc
    14161 taaccagatg aaaaatttgc tcaaaatgtt taaattaact tatgcaacag tcacgcatta
    14221 tattaaataa catttgactg ggttgaacat aacgccaata gcaaaaggag ttatgttcaa
    14281 caatttaatt ttagatatac atataacttg ttctattaat aaaccatctc gtattctgac
    14341 tcgccccctg gcgcagcaaa gtcatgcact tatactcttt tgaatacata gaaaattgat
    14401 atcaatataa tgaaaatatc aaatatttgc atataaatat aatcttaaag tttagtctat
    14461 ttaatgttca atgaaatatt tctacctgta taatctttaa agatgttgaa catatattca
    14521 cattaaatat gattatgtac ttgttacaag gataaggtta taaatgaata aagttacaaa
    14581 aacagctatt gcgggcttac ttgcgctttt cgcaggtaat gccgctgcaa ccgatggtga
    14641 aatagttttt gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa
    14701 aattgaagtt gctcttggtc actataatgc cgaacagttt cgttctgtag gcgatcgtag
    14761 cccaaaaatt ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga
    14821 taacggcaac gttgaagcgt ctttccgttt atggctggaa acgcgggata atggtaccgt
    14881 ccctaacttc cctaacttgg caaaagtggg ctcttttgct ggcacagcag caacaggtgt
    14941 gggtatccgt attgacgatg cggaaagcgg aaaccttatg ccactaaatg ctatgggcaa
    15001 tgataacaca gtttatcaga tccctgcgga ctccgctgga attgtcaatg ttgacctcat
    15061 cgcttactac gtatcaaccg ttgaagcatc cgaaatcacc ccaggggaag cagacgctgt
    15121 cgttaacgta acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa
    15181 cgcccttttc cttcctattt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa
    15241 cattcgcgat gatatttacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg
    15301 gtatcgtttt acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac
    15361 ctgtcgcaaa caaaggcgca gaaacacctt atttactgca atcatgggta gataatatag
    15421 atggtacaag ccgtgcccca tttattataa ccccaccgct aattcgtctt gaagctggcg
    15481 atgattcatc actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc
    15541 tgttctacat taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta
    15601 acgagttgac gctggtattt aaaacacgga tcaaaatgtt ttatcgcccc gcacacctga
    15661 agggacgggt aagcgatgcg tggaaatcac tggaatttaa acgtagtgat cattcactca
    15721 atatatataa cccaactgaa tattacgtcg tatttgccgg actagcagtc gataaagccg
    15781 atctcacaag caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag
    15841 catctggcgg aaagaacgtg aaatgggctg cgatcaatga ttatggcggc agttccggga
    15901 cagaaactcg tccactgcaa taacaaatat aaaaacacag gtcatcaggg aatgccacaa
    15961 cgacaccacc agggacataa acgcacaccg aaacagttgg cgctcattat caaacgctgt
    16021 ttgccgatgg tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat
    16081 tatttcgacc ccattatgct ggaaaccaca aaaagtggta tgcaaacaac cgatctgtca
    16141 cgtttttcaa aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat
    16201 aaaaagaagg tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag
    16261 ccacagttta cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg
    16321 ctggctgaaa aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca
    16381 gctgctgaat ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg
    16441 taccgtgatg caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg
    16501 tttaccaact actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa
    16561 cgacagtacc taaatatgca aaatggtgcc aattttggcc cctggcgatt acgtaactat
    16621 tctacgtgga cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa
    16681 cgtgatatca aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt
    16741 attttttcca gctacaactt tactggcgtg caactcgctt ccgacgataa tatgttgcca
    16801 aacagccagc gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg
    16861 actatcaggc aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa
    16921 attaacgatc tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt
    16981 gacggtacgc aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct
    17041 gggcatctaa aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc
    17101 aaggaacccg aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg
    17161 tatggcggcc tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca
    17221 cttggcgcac tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac
    17281 cagcactctt ttcatggcta tcaatggcgt acgcagtaca tcaaagatat cccggaaacc
    17341 aacaccaata tcgctgtcag ctactatcgc tataccaacg atggctattt tagttttgat
    17401 gaagccaata cccgcaattg ggactataac agtcgccaaa aaagtgaaat tcaattcaac
    17461 atcagccaga caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat
    17521 tggggcaata acgagaaaaa caggaatatc tctgttgggg tttccggcca gcaatgggga
    17581 attggttaca gcctgaatta tcaatacagc cgctacactg atcaaaataa tgaccgcgca
    17641 ctctctttga atctcagtat tccgttagaa cgctggttac cgcgtagccg ggtttcctat
    17701 cagatgacca gccagaaaga tcgcccaacc caacatgaaa tgcgtcttga tggctcactg
    17761 ctggatgatg gtcgcctgag ctatagtctg gaacaaagtc tggatgacga taacaaccat
    17821 aacagtagcg tgaacgccag ttaccgttca ccttatggaa ccttcagtgc cggatacagt
    17881 tacggtaatg acagtagcca atacaattac ggcgttaccg gcggcgtggt tatccatcct
    17941 catggtgtga cgctctcgca atatctgggc aacgcttttg cgcttattga tgctaacggg
    18001 gcatctggcg tgaggataca aaactatccg gggattgcta ctgatccctt tggctatgca
    18061 gtggttcctt atctcacaac ttatcaggaa aaccgtctct cggtagatac tacgcagctg
    18121 cccgataacg tcgatcttga acaaacaaca cagtttgtgg tgcccaacag aggtgcaatg
    18181 gtagcggcgc gtttcaacgc caatatcggt tatcgcgtac ttgttacagt cagcgatcgc
    18241 aacggtaaac cgttgccctt tggcgctctt gccagcaacg atgatacggg gcaacaaagt
    18301 atcgtcgatg agggcggcat actatatctc tctgggatat cgagtaaatc acaaagctgg
    18361 actgtacgct ggggaaatca ggcagatcaa caatgtcagt ttgcttttag tacaccggat
    18421 tcagaaccaa caacctctgt attacaaggc acagcgcagt gccattaagg ataaaaaaat
    18481 gaaaagagcg cctcttataa caggactttt gttgatatcc acatcctgcg cttatgcctc
    18541 ctcagaaggg tgtggagctg acagcactag cggtgcgaca aattacagca gtgtggttga
    18601 tgatgttacg gtgaaccaga cagataacgt gacaggacgg gagtttacct ctgcaacgct
    18661 aagtagcact aactggcaat acgcctgttc ctgctctgcg ggtaaggcag ttaaacttgt
    18721 ctatatggtc agccccgtac ttaccaccac tggacatcag acaggatatt acaaactcaa
    18781 tgacagcctg gatattaaaa cc
//
LOCUS       DAZFBR010000093        18476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFBR010000093 DAZFBR010000000
VERSION     DAZFBR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.093"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            80..697
                     /locus_tag="ACHJZ2_003539"
                     /pseudo
     CDS             80..697
                     /locus_tag="ACHJZ2_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            800..1303
                     /gene="yfbM"
                     /locus_tag="ACHJZ2_003540"
     CDS             800..1303
                     /gene="yfbM"
                     /locus_tag="ACHJZ2_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO4569583.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1370..2827)
                     /gene="nuoN"
                     /locus_tag="ACHJZ2_003541"
     CDS             complement(1370..2827)
                     /gene="nuoN"
                     /locus_tag="ACHJZ2_003541"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO4569584.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2834..4363)
                     /gene="nuoM"
                     /locus_tag="ACHJZ2_003542"
     CDS             complement(2834..4363)
                     /gene="nuoM"
                     /locus_tag="ACHJZ2_003542"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO4569585.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4594..6435)
                     /gene="nuoL"
                     /locus_tag="ACHJZ2_003543"
     CDS             complement(4594..6435)
                     /gene="nuoL"
                     /locus_tag="ACHJZ2_003543"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO4569586.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6432..6734)
                     /gene="nuoK"
                     /locus_tag="ACHJZ2_003544"
     CDS             complement(6432..6734)
                     /gene="nuoK"
                     /locus_tag="ACHJZ2_003544"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO4569587.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6731..7285)
                     /gene="nuoJ"
                     /locus_tag="ACHJZ2_003545"
     CDS             complement(6731..7285)
                     /gene="nuoJ"
                     /locus_tag="ACHJZ2_003545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO4569588.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7297..7839)
                     /gene="nuoI"
                     /locus_tag="ACHJZ2_003546"
     CDS             complement(7297..7839)
                     /gene="nuoI"
                     /locus_tag="ACHJZ2_003546"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO4569589.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7854..8831)
                     /gene="nuoH"
                     /locus_tag="ACHJZ2_003547"
     CDS             complement(7854..8831)
                     /gene="nuoH"
                     /locus_tag="ACHJZ2_003547"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO4569590.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8828..11554)
                     /gene="nuoG"
                     /locus_tag="ACHJZ2_003548"
     CDS             complement(8828..11554)
                     /gene="nuoG"
                     /locus_tag="ACHJZ2_003548"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO4569591.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11607..12944)
                     /gene="nuoF"
                     /locus_tag="ACHJZ2_003549"
     CDS             complement(11607..12944)
                     /gene="nuoF"
                     /locus_tag="ACHJZ2_003549"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO4569592.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12941..13441)
                     /gene="nuoE"
                     /locus_tag="ACHJZ2_003550"
     CDS             complement(12941..13441)
                     /gene="nuoE"
                     /locus_tag="ACHJZ2_003550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO4569593.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(13444..15246)
                     /gene="nuoC"
                     /locus_tag="ACHJZ2_003551"
     CDS             complement(13444..15246)
                     /gene="nuoC"
                     /locus_tag="ACHJZ2_003551"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO4569594.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15340..16002)
                     /gene="nuoB"
                     /locus_tag="ACHJZ2_003552"
     CDS             complement(15340..16002)
                     /gene="nuoB"
                     /locus_tag="ACHJZ2_003552"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO4569595.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16018..16461)
                     /gene="nuoA"
                     /locus_tag="ACHJZ2_003553"
     CDS             complement(16018..16461)
                     /gene="nuoA"
                     /locus_tag="ACHJZ2_003553"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO4569596.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17092..18030)
                     /gene="lrhA"
                     /locus_tag="ACHJZ2_003554"
     CDS             complement(17092..18030)
                     /gene="lrhA"
                     /locus_tag="ACHJZ2_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO4569597.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         4614 a         5132 c         4739 g         3991 t
ORIGIN      
        1 actcataact cattataaat aatgataaaa taacgtcttt tattgataat ttacatatga
       61 aaaaaatttt ccttttgtta tattatttct gttttcactc cctctgatta ttttttacca
      121 accctgggtc actgcattat cgccaacgcc ccgacacgta attcctgagc aattagaaaa
      181 aacagttcgt tatcttacac aaactgtgca tccacgtaat gccgacaata ttgataatct
      241 gaataggtcc gtagagtata taaaaaaagg tctttgtcag tagcggtgcc agagttaccg
      301 cgcagggcgt ccccaattgg ctattacgac tctgcgcctg gtagccagga ttacccttac
      361 ccgcaatgtc ctggctttat cccgatcggg gggactttat tgcctggtcg gcagaataca
      421 ggatatcaac gccgttcgtc aggtaaaaac ggcattgtta tcatcccggg atttatctgt
      481 ttattctatg aatgccccat ggtttattca cggtattgat ttctctgacc accttaatta
      541 ttggcaacac gatattctcg ccgtaatgat tactgacacc gctttttatc gtaataaaca
      601 ataccacttg cccggtgata cagcagacag attgaattat cagaaaatgg ctcagatggt
      661 ggatggtgtt ataactttgt tacacaacag taaataataa aaatttctcg aggcgggtcc
      721 agaaacagcg acatgtacat tttattttcc ctcgtctgtt ttaagatgcg aaacataaaa
      781 ttcaatggag gaaaaataaa tgggaatgat tggttatttt gcagaaattg actcagaaaa
      841 gattaaccaa ctgcttgagt ctactgaaaa acctttgatg gataatatac atgacacgct
      901 ttcaggatta cgccgactgg atattgataa acgctgggat tttttacact ttgggctgac
      961 aggcacctct gcgttcgatc cagcgaagaa tgatccgctg agtcgtgccg tgcttggtga
     1021 acacagcctc gaagatggta tagatggttt cctgggattg acatggaatc aggagttagc
     1081 cgcgacaatc gaccgactgg aatcactgga tcgaagtgag ctgcgtaaaa agttttccat
     1141 taagcgactc aatgaaatgg agatttatcc gggagtgacc ttttcagaag agttagaggg
     1201 gcaactcttt gcttcgatca tgcttgatat ggaaaagctg attagcgcgt atcgacgtat
     1261 gctgcgacaa gggaatcatg cgctgacggt tattgttggg tgatggtgtc agggcaccaa
     1321 aaaaaaccgc cgaacatgtc ggcggctttc tgacttacaa actgatagct tacatcagcg
     1381 gcattgctaa ccgcacaata ctaatcagcg gttgtggcca tacacccagc accagtacca
     1441 acagtgcaga aatcagcacc acgataccgc ccgcgctgta ctgccagttt gacggtgcat
     1501 cgcgacccgg ttgttccggt gcgtgcaggt acaggctcac cgccacgcgc aggtagtagt
     1561 agaggccgat tgccgaaccg acaaccacgg cacccaccag ccaccacagg tgtgcctgga
     1621 caccaaccgc cagcacgtag aacttaccga taaagcccag cgtcatcggg ataccggcca
     1681 gagacagcat catcaccgtc atcaccgccg cgaggatcgg acgatgccag aacagaccgc
     1741 ggtaagagaa cagggaatca gcatccgggc cacgatacgg gctggacatc aggctgacca
     1801 cgccgaacgc gccgaggctg ctgaacagat aaccggccag gtaaacccct accgcttcca
     1861 tcgacatctc gccagtttgc agcgcaatca gcgccaccag cagatagccg aggtgagaga
     1921 tagatgagta accgagcaga cgtttgatat tggtctggct cagcgccatc aggttaccga
     1981 agatgatgga ggcaaaggcg ataatcgcca gcaccacccg aatcgcttcg ctgtcaccca
     2041 ccggtgcgta gaggaacaga cgcatcacca caccgaagat agcgattttg ctcgccgtcg
     2101 ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac gtctggcgtc cacaggtgga
     2161 acggcaccag agagagtttg aagccgaggc caacaatcat caggccgaaa cctgccagca
     2221 acagcggctc gtttagcata ccgtcgccga ggtttttacc cagcgcgaca aacgacaggt
     2281 cgccagactg cgcatacacc agcgccatac cgaacagcag gaaagaagac gctgcggcag
     2341 aaaggatggt gtatttgata ctggcttcca gcgaacgttt ctggcggaaa gcgtaaccga
     2401 ccaggccaaa cagcggcaaa gagatcagtt caataccgag gaacagagac gccagatggt
     2461 ttgcattcgc cagcaggatc ccgcccagcg cggcaattaa caccaacagg tagaactcat
     2521 ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa agtacaggtg gcgaggctcg
     2581 ccaacaatac cagcccggtg taaagcatgg cgaaaccatc aacgcgcatc agcggcgtaa
     2641 cgtccatagc gcccgcctgg ccaacaaacc agagcgaaac cagcgccgcg ttaagcccaa
     2701 taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca cgcaatggag agcatcacaa
     2761 ccaccaccgt caagccgacg atcagcaacg gtagcagtgc gatcaggttt tgtggagtta
     2821 ttgtcatggc gatttacggc cttgtagtag taacggaatt aacaaaccac tgctggatat
     2881 tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg atagaagccc agcagtacca
     2941 gcagcaccac cagcaacagg atcataaaca gctcacgcag cgacatccct ggcagttcct
     3001 ggctggcaat ctggcttttc gctttaccga agtaagcgcg atgtaacatc gccagcgaat
     3061 aaacagatgc aaagaccagc ccaaaggtag agataacggt aatcaccggg acaacctgga
     3121 agctgccgaa cagaatcata aattcgccga cgaagttacc ggtgcctggc atcccgagcg
     3181 ttgccaccgc aaagaacagt gacaaggctg gcagccattt catcttgctc cacagaccgc
     3241 ccatcatgcg catgtcgcgg gtatggatac gttcataaag ctgaccacaa agaataaaca
     3301 gacccgccgc tgacaagccg tgcgcaatca tctggattac cgcgccctgg taggccaact
     3361 ggctgccggt gtagatagca atcagtacga agcccatgtg ggaaaccgag gtgtaggcga
     3421 tcagacgttt gatatcggtc tgggcgaagg ccatccacgc accgtagaag atgccgataa
     3481 cacccagcca catggcgatt ggcgcgaact ctgccgacgc gtttgggaac agcggtaggg
     3541 agaaacgcag cagaccgtaa gcggcggttt tcagcaagat ccccgcgagg tccacggaac
     3601 cggcggtcgg tgcctgtgag tgcgcatccg gcagccagcc atgcagcgga accaccggca
     3661 ttttcaccgc aaacgcgatg aagaagccca gcatcaacag atattccaca ccactggaca
     3721 ttggcgtatt cagcagctct tcatagttga aggtccagac gccggtcgca ttgtagtgaa
     3781 caaaaaccag cgccaggatg gcgatcaaca tcaccagacc actcgcctgg gtgtaaatga
     3841 agaacttggt tgccgccgtg atacgcgttt taccatcaga ggctttatgc ccccacagtg
     3901 cgatcaggaa gtacatcggc accagcatca tttcccagaa gaagaagaac aggaacatgt
     3961 cgatggcaag gaacacgccg ataacgccgc ccaggatcca catcaggttg aggtggaaga
     4021 agccctgata tttttcgatc tctttccacg aacagagtac cgccagcaca ccgagcagac
     4081 cggtcagcac gaccatcagc agcgacagcc cgtcaatggc gagatggata gagataccaa
     4141 aacgcgggat ccacggcatg tcgaattcag actgccactg cggaattccg gcggattgcg
     4201 tcagtgaata accgccctgc aaccacagtt gcagcgacag cgccagcgtc aatcccatgg
     4261 tgatcagcgc gatccagcgc ggcaccttga cgccaaagcg ttcggtctgc cagcacagga
     4321 agccgccgat aaaggggatt agtattaacc agggtaataa catagctaag tatgtccctt
     4381 atatttactc ctgctcaggc tatctcactc gccagcccga atcccggcag tgctcgcaat
     4441 cctcacgtac ttaagtacgc tccggttgct gtgcgctggc ggtatccggt ctgacttcgc
     4501 cgatgacgcc tgtacttcag gaacagttac ctaaaattta ttcttagttc acagacccgg
     4561 cgcaacgccg ggtatattca cccagccaac aactcaacgc agtaccatca acagtgccag
     4621 caccacgacc gcaccgatgc tcatggatgc cacataccag cgcagataac cgttctcgct
     4681 taacagcagg cctttacctg caaagcggga aaggacagcc gggatgttca tcattgagtt
     4741 cagcggatcg cgtttcagca accaggcaat acccaggaac ggcttgacga acactttgtc
     4801 gtacagccag tcaaagcccc aggcgttgta ccaccaggtg cccagcagac ggcccggcgc
     4861 actgttggcg atggaggtca ccagagtacg tttacccagc cacagccagg ctgccagcag
     4921 aatgccgacc accgcgacca cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc
     4981 cagttccgtc gtttgcggaa gcacgccctg cagcggcggt acaatcagtg cgccaacgaa
     5041 ggtggaaagg atcagcagca caatcagcgg caggctgtga gttacccctt tcacggcgtg
     5101 agcgtgaatt tgttcttttc cgtggaagac gatgaaaatc atacggaagg tgtagagcga
     5161 ggtcataaac gcaccgacca gacctgccac catcagattg atatgaccat tcgccatcgc
     5221 acccgcgagg atctcatcct tactgaagaa gcccgcagtg actagcggca gtgccgacag
     5281 tgctgcgccg cccaccagga agcagagata aaccagcgga atagatttac gcagaccgcc
     5341 catcttgaag atgttctgtt cgtgatggca ggcgagaatg acggaaccgg atgccaggaa
     5401 cagcagcgct ttaaagaacg cgtgggtcat caagtgaaaa atcgccgcat cccatgcctg
     5461 cacgccaagc gcgaggaaca tgtagccaat ctggctcatg gtagagtaag cgagaacacg
     5521 tttgatgtcg gtctgtacca gcgcggcaaa accggccagc agcagcgtaa ccgccccaac
     5581 aatgcccacc agatgcagaa cttccggcgt catcaggaac aggccgtggg tacgggcgat
     5641 caggtagaca cccgcggtta ccatcgttgc ggcgtggatc agcgcggaga caggcgtcgg
     5701 gcccgccatc gcgtcggcaa gccatgtctg caacggcaac tgcgcagatt taccgaccgc
     5761 accgcccagc agcatcagcg tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc
     5821 tggtgccagt tccaccattt cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa
     5881 cagcgcgaaa gcgaggaaca cgtcacccac acgggtcacg acgaacgctt tcattgccgc
     5941 tgcgccattc ttcggatcgg tgtaatagaa cccgatcagc agataggagc acaggcccac
     6001 gccttcccag ccgaggtaca tcagcagcag gttgtcggca agcaccagaa ccaccatgct
     6061 ggcgataaac aggttggtgt aagcgaagaa gcgagagtag ccctcttcac cgcgcatata
     6121 ccaggaggcg tacatgtgaa taaggaaacc cacaccagtg accaccgaga gcatggtcag
     6181 cgacaggccg tccagcacca ggttaaaacc gatgttaaag tcgcctaccg acatccacgt
     6241 ccacagcggc tggctgtatg cctgctcgcc gttagcgaag aagtcaacgc caataaaggc
     6301 agttaccagc gccgccaggc ccacagagcc aacgccgacg atcgccgaga cgttttcaga
     6361 ccagcgccca cgggagaacg ccagcaggac gaagccaatc aatggcaaaa taatggttaa
     6421 ggcaagcatg ttcatccgcg catctcactt actgaatcga tgttcaggtt ctggcgacga
     6481 cggtgaagtt gcagcagcag cgcaaggccg atactcgctt ctgccgccgc gaggctgatg
     6541 gcgagaatgt acatcacctg accgtcggtc tggccccagt aacttccggc gaccacaaag
     6601 gccagcgcgg aagcgttaat catgatttcc agaccaatca gcataaacag cagattgcga
     6661 cggataacca gaccggttaa gccaagaacg aataagattg ccgcgaggat cagtccatgt
     6721 tgtaagggga tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct ttacgattgc
     6781 tcagcacttc acccgcacgc tcttcacgac cgacgtggaa ggccacaacc agacccgcga
     6841 gcagcagcat agaagccagt tccaccgcca gtacgtaagg cccgaacagc gtaataccca
     6901 ctgctttagc actgattggc gtaccgtcga taccttgatc gttaacaccg agaatggcgt
     6961 aaacaatcac caccaacatg atggctgaca aaattgccgg accaatccac acctgcggtt
     7021 tcagccactg gcgttcttgt tcgatttctg aaccgcccag gttgagcatc atcaccacga
     7081 acacgaacag caccataatg gcacccgcgt agacgataat ttccagcgca ccggcgaagt
     7141 aagcgcctag tgagaagaac accccggaaa tcgccagcag cgaaataatc aggtacagca
     7201 gtgcgtgtac cggattggta tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc
     7261 cacagatata aaaagcgaac tccattgccc ctctccttac ggtaacaggc tcttgacgtc
     7321 gataggcttg gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc
     7381 catccggtag aagttatact ccgggtattt gcccggaccg gagatcagca gatcctcttt
     7441 ctcgtaaacc agatcctggc gcttgtattc ccccatttcg aaatccgggg ttaactgaat
     7501 cgccgtggtc ggacaggctt cttcgcacaa accacagaaa atgcagcgtg agaagttgat
     7561 gcggaaaaat tccggatacc agcgaccgtc tttggtttct gctttttgca gcgagataca
     7621 gccgaccggg caggctaccg cgcagaggtt acaggctacg caacgctctt cgccgtccgg
     7681 gtcgcgggtc agaacgatac gaccacgata acggggcggc agatagaccg gctcttccgg
     7741 gtacattcgc gtttcgcgtt tggcgaacgc gtgcaggccg atcatccaga tactacgaac
     7801 ctgggtgccg aaacctacta acaattcttt taaggtcatg gtcttattgc cccttattgc
     7861 gcctgccaga gaatgacagc cgccgttacc agcaagttga tcagcgtcag cggcaggcag
     7921 attttccagc cgaaggacat tacctggtca taacgcggac gcggtaacga cgcacgaatc
     7981 aaaatgaaca tcatcataaa gaacgcggtt ttcagcgcga accagatgaa tggcggtaac
     8041 aacgggcctt gccagccacc gaagaacagc gtcaccatca atgcagagat ggtcacaatc
     8101 ccgatgtact cacccacgaa gaacagaccg aacttcatac cggaatattc aatgtggtaa
     8161 ccatccgcca gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg acacaccgcc
     8221 acgcccgcga tggcaaaggt aataaaacca aagaattgcg ggatgacgtt ccacacatgc
     8281 gcctggctgt tgacgatgtc ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc
     8341 agggaaagcc cgaggaacac ttcgtagctc agggtctgcg cagaagcacg catcgcaccg
     8401 agcagcgagt atttgttgtt acttgaccag cccgcaaaca gcaccgcgta aaccgccaga
     8461 ccggccatca tcaggaagaa caaaatcccg atgttcaggt cggcaaccac ccaacccgga
     8521 ctgactggca caatcgcaaa ggccagcagc agcgaggtaa aggcaatcat cggtgccagg
     8581 gtaaagatga cgcgatccga gaatttcggg atccagtctt ctttaaagaa cattttgatc
     8641 atgtccgcaa ccagctggag cgaaccaccc cagccaacac ggttaggtcc gtaacggttc
     8701 tggaacagac ccagcaggcg acgttcgcca aagctcatga atgccccgca ggtgacaacc
     8761 accagcagga tcaccaccgc tttgaggatg gtcagcagga tctcaatcag ttccggtgat
     8821 atccaactca ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc agcaccggag
     8881 caatgccgga catacccatc ggcaagccca cctgccctgc cgtcagccct tcagcgattt
     8941 caaccggcag agtgaccgtg ttgccatcgt aactaaagga gacgcgtgta cctgcgttta
     9001 cacccaactt cgcggcatcc gctgggttga gtttgatgta cggctgcggc atacgacact
     9061 ggaagaccgg agcacgctgt gacaattcat cgctgccaaa caggtggtag tacggcgcga
     9121 tacgccattt cccgtcctgc ggctggaagc gtgccggtac gctggtgaag taatccagac
     9181 cattttcgct ggtttcaaac agacgcacac ccggatcgcc aaagcgcagt ttgccgccca
     9241 cttcgtcctg gaatttgttc cacgcctgcg gggagttcca gcccggagcc caggcaaacg
     9301 gcacttgcga acggtgcgcg gtcggctggt tgttaccttc catcgagaag gtgaacatgg
     9361 tgtcaatatc ctgcggctga cgcggctcat gaacgctgat attggcgcgc atggcggtac
     9421 gaccgctgta acggtgcggt tcacgggcca gtttctgccc acgaatacgg aatgtcgcat
     9481 ctggcgcagc atctttgata cccgccagtt ccgggatttt cgccacaaca gcgtcaatta
     9541 catggtcgag ctgcgtccag tccacttcac ggctcagcaa ggtgctgtgc agcgagtgca
     9601 gccagcgcca actttccagc atgacagttt tgctgtcgta ataagccgga tcgtaaacct
     9661 ggaagaaacg ttgggcgcga ccttcattat taattaccgt accgtcgctt tcagcaaagc
     9721 tggcagcgga aagcaccaga tgggcgtttt ccatgatcgc tgtgcgttga tggtcaacca
     9781 ccatcaccag cggcgcttta gccagcgcag cattcacgcg ggtagcagaa gcgtgacgat
     9841 gcagatcgtt ttccagcacc accaccgcgt cggcgcgtcc ggtttccagt tcggttaacg
     9901 cttcttcaag cgatccaccg cccataatgc ccaaccccat gctgttgacg gaacgggcaa
     9961 tcatggtgat accgacgtca gcgccgcgac ctttcagggc tttcgcgacg ttagccgcag
    10021 cctgaatcac ctctaagcta ccggcgttcg tcccggagat aatcaacggt ttcttcgcac
    10081 ccgccagtgc ctgcacgatg acgtcgattt tgctttgcag ctcaggttca ataccgtcaa
    10141 ccgctggcgc agagttatcc agcgcatggg cgatggcaaa acctaaacgc gcctgatctt
    10201 caaccggtgc gcggtaagtc cacgccgcga tatcatccag acgggtgtca tcaacgttgg
    10261 taacaaacag cggatgctta gcacgttgac cgatattgag gattgccgca atctgccagt
    10321 cagccacttt ctgtgctgcc gccatttcgc gcgctttacc tttcaccgcc tggcgcactg
    10381 ccagcgcgac gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt accgcatcgt
    10441 aagattcgat ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagc actttcagcg
    10501 ccagttgcag acgttcctgc tcaccgtgag cgataccggt gtagaagttt tcttcgccca
    10561 ccagttcacg cagcgcaaag ttgctttcca cgctggcacg cggagaacca ataccgatca
    10621 ctttcttcga ctgacgcaga atatctgccg cgccctgcat tgcctgttcg gcgttgaggg
    10681 taatgaaatc atcgccacga cgctgtactg gctgacgcgg acgatctttc aggttgacgt
    10741 aaccgtaacc gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac
    10801 ggttttcgat acgacgcagt tcgccataac gctcgccggg gctgatgtta cagccgatgg
    10861 aacattgctg gcagatgctc ggcgcaaact gcatatccca tttacggttg taacgctcgg
    10921 agtgcgtttt gtcggtaaat acgccggtcg ggcaaatttc gaccaggtta ccggaaaatt
    10981 cgctttccag cgtgccgtct tccgggcgac cgaagtagac gttgtcatgc gcgccataaa
    11041 cgcccagatc tgtaccgtca gcgtaatctt tgtagtaacg cacgcagcgg tagcaggcga
    11101 tgcagcggtt catttcgtga gagatgaacg gccccaaatc ctgattacgg tgggtacgtt
    11161 tggtgaaacg gtagcgacgg aagctgtgtc cggtcatcac ggtcatatcc tgaagatggc
    11221 agttaccgcc ttcttcacat accggacagt cgtgcgggtg gttggtcatt aaccactcga
    11281 ccacactttc acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg
    11341 aagccggtgt catacaggac atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt
    11401 gcttcaccgc acactggcgg caagcaccga cgcttcccag cgccggatgc cagcaaaagt
    11461 aaggaatatc aaggcccaga gacagacaag cttccagcag gttgtccgct ccgttgacct
    11521 cgtattcttt gccgtctaca tgaattgtag ccattagcat gcttccagtt ttctcagtca
    11581 gagactgagc gttaatcgaa attcggttac cagcgctctt tcagcaggtt cggctgaatc
    11641 ccattaatca aatgggtatt gctgaacggc tgtttgatcc ccgcctcaaa ttcttcgcgg
    11701 aaatatttga tggcgctctg taacggctcc acagcaccag gtgcgtgggc acagaaagtt
    11761 ttacccgggc ctaagaatcg acacagttgc tcaagtgttt cgatatcgcc cggctgacct
    11821 tcgccacgct ccagcgcacg cagaattttc acgctccacg gcagaccgtc gcggcacggc
    11881 gtacaccagc cgcaggactc acgggcgaaa aactcttcca ggttacgcac cagcgacacc
    11941 atgttgattt catggtcaac cgccatcgcc agcgccgtac ccagacggct gcccgcttta
    12001 ccgatacttt cgaattccat cggcaggtca aggtgcgctt cggtcaggaa gtcggtgccc
    12061 gcgccgcctg gctgccaggc tttgaatttc agaccgtcac gcataccacc ggcgtaatct
    12121 tcgaggatct cgcgtgcggt ggtgccgaac ggcagttccc acagtcccgg atttttcacc
    12181 cgaccggaga agcccatcag cttggtgcca gcatctttac ttttcgagat gttctgatac
    12241 cactccacgc cgttagcgag gatcgccgga acgttacaca gggtttcgac gttgttgaca
    12301 caggtcggtt tgccccatac gccggaggtt gccgggaagg ggggtttcga gcgtgggtta
    12361 gcacgacgcc cttccaggga gttgattaac gctgtttctt ccccgcagat gtagcgccct
    12421 gccccggtat ggacgaacag ttcgaagtcg aaacctgttc ccataatgtt tttgccaagc
    12481 aatcccgctt cagtcgcttc ggcaatggcg cggcgcagat ttactgccgc ttcaatatat
    12541 tcgccacgca ggaagatgta gccacggtaa gctttcagcg caaacgcgga gatgagcata
    12601 ccttccacca gcaggtgcgg cagttgctcc atcaacaggc ggtctttata ggtgcccggc
    12661 tccatttcat cggcgttaca cagcaggtaa cggatgttca tggattcgtc tttcggcatc
    12721 aggctccact tcaagccagt cgaaaagccc gcgccgccgc gccctttcag accagcgtct
    12781 tttacctgat taacgatttc gtccggagac agcccggtca gcgccttacg cgccccttcg
    12841 taaccgtttt tgctgcggta ttcgtccagc cacactggct gtttgtcatc gcgcagacgc
    12901 caggtcagcg gatgcgtttc gggagtacgg ataatgtttt tcatttatac cgctccagca
    12961 gttcagggat cgcttccggg gtcagattcg cgtgagtgtc ctcatcgatc atcatgtttg
    13021 gccctttatc acagttcccc aggcagcaag ttggcagcag cgtaaagcgg ccatcaaatg
    13081 tcgtttgccc tggtttgatg ttcagctttt tctcgagcgc cgcctgaata ccctgatacc
    13141 cattgatatg acagaccacg ctgtcgcaat aacggatcac atggcggcca accggctggc
    13201 ggaagatctg gctgtagaac gttgccacgc cttcgacgtc gcttgccgga atacccagca
    13261 catcggcgat cgcgtggatc gcaccatccg gcacccagcc acgctgcttc tgaacgattt
    13321 tcagcgcttc aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg tgctcgatcg
    13381 cttcacgctc tgccgcactc agctcaaaag cctcggtttg tggttgttga ttctcgtgca
    13441 taattagcgg tccacatctg acataacaaa atcgatactg cccagataaa caatcaggtc
    13501 agacaccagg ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt
    13561 gcggatacga gtgcgataac tcatggtgct accgtcgctg gtcaggtagt aactgttgat
    13621 ccctttggtc gcctcaatca tctggaagga ttcattggca ggcatcaccg gcccccacga
    13681 cacttgcagg aagtgggtga tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg
    13741 cggcgtggtc agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg
    13801 ctcaagaatg cgcagactct ggcgcagctc ttccacttta agcattacgc gggtgtagca
    13861 gtcagaaaca ccaccaccaa ccgggatttc aaagtcgaag ttttcatagc cagaataagg
    13921 acgcgcctta cgcacgtcga agtcgatccc ggtagcacgc aggcccgcgc cagtggtgcc
    13981 ccactccagc gcctctttcg cgccataggc ggcaacgccc tgggaacgac ctttcagaat
    14041 ggtattttgc agcgccgctt tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa
    14101 ctcacgcagc aggcgatccc agccgcgcgg caggtcgtgc gctacgccgc caatacggaa
    14161 ccacgccggg tgcatacgga aacccgtgat cgcttccacc agatcgtaaa ttttctgacg
    14221 atcggtaaag gcgaagaaca cgggcgtcat tgcgccgacg tcctgaataa aggtcgagat
    14281 atacagcagg tggctgttga tgcggaacag ttcggagagc attacgcgaa tgacgttaac
    14341 gcgagccggc acggtgatcc cggccagttt ctctaccgcc agcacgtaag gcatttcgtt
    14401 aacgcagccg ccgaggtatt cgatacggtc ggtatacgga atgtagctgt gccaggactg
    14461 gcgttcgccc attttctccg caccacggtg gtggtagccg atgtctggta cgcagtcgac
    14521 aatctcttcg ccgtcgagct gcaaaacgat acggaaagca ccgtgcgccg acgggtggtt
    14581 cggaccgagg ttgaggaaca tgaagtcctc gttttcggtg ccgcgcttca tcccccactc
    14641 ttccggtttg aaggtcaggg cttccatctc cagatcctgt ttggctttgg tcagctcaaa
    14701 cggcgagaat tcggtagcgc gcgccgggta atctttacgc agcgggtgac ctttccaggt
    14761 ttgcggcatc atgatgcggc gcaggttcgg gtgaccgtcg aatgtaatgc caaacagatc
    14821 ccaggtttca cgctcatacc agttagcgtt cgggaacagt ttggtgaagg tcggtacgtg
    14881 caggtcgttt tctgccagcg ccaccttcag catgatgtcg cggttacgat cgatagaaat
    14941 cagatggtag aaaacggaaa aatccgcggc aggtaaccct tcgcggtgcg tgcgcagacg
    15001 ttcgtccatg ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt tctttaagaa
    15061 atcgccaact tccagtaatt gttcacgctt gatccacaca acgggaaccc cggtgcgagt
    15121 cgcctgaaca gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa ttaccggatc
    15181 atcaagatga tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcgg tcatattgtt
    15241 caccattgca aatggtccgt ggtgactgtc ggcgcagcaa gcttcgcgaa atagatttag
    15301 gaataagcga agaaaaatcc ctttgccgac aggcgcaaat taaatctcgt caggtgtacg
    15361 caggttggtt acggcaatgc gttcaccgcg cttgcgttcg cgctctgatt gcatattggc
    15421 gcgataaacg ccctgatcgc caaccaccca ggagagcgga cgacgttctt tgccgataga
    15481 ttcctgcaac agcatcagtg cctgcatgta cgcttcagga cgcggcgggc agcccgggat
    15541 atacacatca accgggatga atttatcgac gccctgcaca acggaataaa tatcgtacat
    15601 accaccagag ttggcacagg cacccattga gataacccat tttggttcca gcatctggtc
    15661 atacagacgc tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag
    15721 gtcagcctga cgcggcgaag cacgcaatac ttctgcgcca aaacgcgcca cgtcatgcac
    15781 cgcggtaaac gaagtcacca tctcaacgta acagcaggaa agaccgaagt tatacggcca
    15841 aattgagttt ttacgacccc agttaaccat gtcattgagc ttgcccataa acacgttttt
    15901 gttaacttct tgctccagag ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc
    15961 gttctcaccg ttgggatcta tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta
    16021 gcgttgacga ttagcgatac tgttcgtttc cgggttcata cgctcgcggc gtgaacgcgc
    16081 gggcgtccag tccagcgcgc caatacgcac cagataaacc agacctgcca gtaacacaaa
    16141 aataaaaatt gcagcttcca caaagcctac ccagccgctt tcgcggatag aggttgacca
    16201 tgcgaacaga tacagcgctt caacgtcgaa gataacgaag aacatggcca ccagataaaa
    16261 cttggcggac aggcgtaagc gggcggagcc gaccgagtcg ataccggatt caaacggcac
    16321 gtttttcgac ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca
    16381 acataggcca atggcaacga taagaaagat agcgaatgcc cagtgatgag cgatgacttc
    16441 agtggatgtt gacatactca ttgcttactc atcaaaagta gcgccagatt cactgctctt
    16501 cacggcagat ggacgccaca tcgattcatg gggaggaata aaaaaaacct tacaatcact
    16561 gtagaaattc ttttatacag ctaattgatg tggtctttta ctcctttcta taaccttttg
    16621 tcaactttaa caaaagtttc ttcacattag tttacataat atcaacacca ttagcattta
    16681 atgccctttc accccagatc cttgacgact ccaggataat tagatgttgt tgaatcgtgt
    16741 ccgttgtgaa gcaatggaaa aaatacgggt ctattttgac aggaatttgt gtcgattcct
    16801 ccccccaaaa gagagtattt tcttgatctg tgacacgctt ttgtcattcc ataacaaaaa
    16861 cgcagcaaca aatttacgta ttttttaaca tcattgtagc aggtgatttt tttcaggcga
    16921 ttatttgtgc gtacggggcg tgaatctctg gtgattgaaa aatgaacagt tttgtacgtt
    16981 ctgcacgatg tgaagaaggt tctttactaa aaaaaagccg ctggggttta aaacaccccc
    17041 agcggctcgt tttttacact attgtctcag gaattatcta tcgtccgtcg attactcgat
    17101 atccctttca atcaacaatg aatcatcccc ttccggagca gatattgggc tgtattgcca
    17161 cggattatgg tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga
    17221 cgggtcatag cacagcaaat attcggtatc aggtagcggc ggtaagccat ctactccact
    17281 cagcacacgc aagtccggac tcatcatctc gaccggcctt gccgtcacgc caagaccggc
    17341 tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc gccagggaat
    17401 atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat catccagcaa
    17461 tacaagaggg atcggctcac ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt
    17521 tggcgaggta cgcaggttca atgccttaaa cgctgaagga cgatgggtgg ttaccatcaa
    17581 atccacttcc tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc
    17641 cagcgctaat ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc
    17701 ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga acattaatga
    17761 tgagcaggcc tcatcattaa aacgcaggat tttcctggcg taaccaagaa gttgaatgcc
    17821 atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg
    17881 ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc
    17941 ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag
    18001 gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga
    18061 gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca
    18121 gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact
    18181 cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag ggatcgcata
    18241 tctattaagt tactcactct tttctattta tgacatgcgc gtgtttgtat aaatgtaaat
    18301 gtgagtcctt gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag
    18361 caatggtgag gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa
    18421 aaccttttat ttataagggt cattgcgaat tatctgatgc aaagttatgt tatgtt
//
LOCUS       DAZFBR010000094        18249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFBR010000094 DAZFBR010000000
VERSION     DAZFBR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.094"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            42..1181
                     /gene="arnB"
                     /locus_tag="ACHJZ2_003555"
     CDS             42..1181
                     /gene="arnB"
                     /locus_tag="ACHJZ2_003555"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO4569598.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1185..2153
                     /gene="arnC"
                     /locus_tag="ACHJZ2_003556"
     CDS             1185..2153
                     /gene="arnC"
                     /locus_tag="ACHJZ2_003556"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO4569599.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2153..4135
                     /gene="arnA"
                     /locus_tag="ACHJZ2_003557"
     CDS             2153..4135
                     /gene="arnA"
                     /locus_tag="ACHJZ2_003557"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO4569600.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4132..5022
                     /gene="arnD"
                     /locus_tag="ACHJZ2_003558"
     CDS             4132..5022
                     /gene="arnD"
                     /locus_tag="ACHJZ2_003558"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO4569601.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5022..6674
                     /gene="arnT"
                     /locus_tag="ACHJZ2_003559"
     CDS             5022..6674
                     /gene="arnT"
                     /locus_tag="ACHJZ2_003559"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO4569602.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6671..7006
                     /gene="arnE"
                     /locus_tag="ACHJZ2_003560"
     CDS             6671..7006
                     /gene="arnE"
                     /locus_tag="ACHJZ2_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO4569603.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7006..7392
                     /gene="arnF"
                     /locus_tag="ACHJZ2_003561"
     CDS             7006..7392
                     /gene="arnF"
                     /locus_tag="ACHJZ2_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO4569604.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7386..7652)
                     /gene="pmrD"
                     /locus_tag="ACHJZ2_003562"
     CDS             complement(7386..7652)
                     /gene="pmrD"
                     /locus_tag="ACHJZ2_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO4569605.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(7762..9117)
                     /gene="menE"
                     /locus_tag="ACHJZ2_003563"
     CDS             complement(7762..9117)
                     /gene="menE"
                     /locus_tag="ACHJZ2_003563"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO4569606.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9114..10076)
                     /gene="menC"
                     /locus_tag="ACHJZ2_003564"
     CDS             complement(9114..10076)
                     /gene="menC"
                     /locus_tag="ACHJZ2_003564"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO4569607.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10076..10933)
                     /gene="menB"
                     /locus_tag="ACHJZ2_003565"
     CDS             complement(10076..10933)
                     /gene="menB"
                     /locus_tag="ACHJZ2_003565"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO4569608.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10948..11706)
                     /gene="menH"
                     /locus_tag="ACHJZ2_003566"
     CDS             complement(10948..11706)
                     /gene="menH"
                     /locus_tag="ACHJZ2_003566"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO4569609.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11703..13373)
                     /gene="menD"
                     /locus_tag="ACHJZ2_003567"
     CDS             complement(11703..13373)
                     /gene="menD"
                     /locus_tag="ACHJZ2_003567"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO4569610.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13462..14757)
                     /gene="menF"
                     /locus_tag="ACHJZ2_003568"
     CDS             complement(13462..14757)
                     /gene="menF"
                     /locus_tag="ACHJZ2_003568"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO4569611.1"
                     /translation="MQSLTTALENLLRHLSHEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14836..15141)
                     /gene="elaB"
                     /locus_tag="ACHJZ2_003569"
     CDS             complement(14836..15141)
                     /gene="elaB"
                     /locus_tag="ACHJZ2_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO4569612.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15196..15657)
                     /locus_tag="ACHJZ2_003570"
     CDS             complement(15196..15657)
                     /locus_tag="ACHJZ2_003570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4569613.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15722..16639
                     /gene="rbn"
                     /locus_tag="ACHJZ2_003571"
     CDS             15722..16639
                     /gene="rbn"
                     /locus_tag="ACHJZ2_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO4569614.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16827..18050
                     /locus_tag="ACHJZ2_003572"
     CDS             16827..18050
                     /locus_tag="ACHJZ2_003572"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFO4569615.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATK
                     HPLKSHDSDNLLFKKLFSPSKLMAIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFW
                     DKNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWP
                     KEVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFNSLRALGHEKENSLKRIINSF
                     NSFNCDPTRETPNNKNITDHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQMES
                     NSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLENRNCYLDKYGDANINHYYRNLEI
                     KNSHPKNRASSKRVS"
BASE COUNT         4413 a         4855 c         4544 g         4437 t
ORIGIN      
        1 aaatataagt ggacaggtat tcaatggcgg aaggaaaagc aatgtcagaa tttttgcctt
       61 tttcgagacc agcaatgggc gtggaggaac tcgctgcagt taaagaggtt ctcgaatccg
      121 gttggatcac aaccggtccg aagaatcacg cgcttgaaca agccttttgc cagttgacgg
      181 gaaatcagca tgccatcgcg gtcagttcag ccaccgccgg aatgcatatc acgctaatgg
      241 cgttggaaat tggcaagggc gatgaagtga ttacgccttc cctgacctgg gtttcaaccc
      301 tcaatatgat ttccttgcag ggtgcaacgc cggtaatggt ggatgtcgat cgcgatacgc
      361 tgatggtcac gcctgaagcg atcgaatcag ccattacgcc acgcactaaa gccatcattc
      421 cggtgcatta tgccggtgcg ccagcagata ttaacgccat tcgcgccatt ggcgaacgtt
      481 acggcatcgc agttatcgaa gatgctgccc atgccgtagg tacgtattac aaagggcgac
      541 atattggcgc aaaaggtacc gctatttttt catttcatgc cattaaaaat attacctgtg
      601 cggaaggagg cctgattgtt actgataatg aaaatattgc ccgccagcta cggatgctga
      661 aatttcacgg cctgggtgtc gatgcctatg acagacaaac ctggggccgt gcaccgcagg
      721 ctgaagtctt aacgccgggc tataagtaca atctgaccga tattaacgcc gcgattgccc
      781 tgacacagtt agccaaatta gagcacctca acacccgtcg gcgcgaaatt gcccagcaat
      841 atcagcaagc actggcagct ctcccctttc agccattaag ccttcccgcc tggccgcacg
      901 ttcacgcctg gcatctgttt attattcgtg tcgatgaaca acgttgtggt atcagtcgcg
      961 atgcgttgat ggaagcgtta aaagaaagag gtattggtac cgggttacat ttccgcgccg
     1021 ctcacacaca aaaatattat cgcgagcgtt ttccctcgct gtcgttaccg aataccgaat
     1081 ggaatagcga acgcatctgt tctttgccgc tgttcccgga tatgactacc gccgatgccg
     1141 accgtgtcat cacagccctt cagcaactcg caggacaata agccatgttt gaaatccacc
     1201 ctgttaagaa agtctcggtg gttattcccg tttataacga gcaggaaagc ttaccggaat
     1261 taatcaggcg caccaccaca gcctgtgaat cgttggggaa agagtatgag atcctgctga
     1321 ttgatgacgg cagtagcgat aattccgcgc atatactggt cgaagcctca caagcggaga
     1381 acagccatat tgtatctatt ttgcttaacc gcaattacgg gcaacattca gcgattatgg
     1441 cgggattcag tcacgttact ggcgacttaa ttattaccct tgatgccgat ctccagaatc
     1501 cgccagaaga aatcccccgc ctggtggcaa aagccgatga aggttacgac gtggtaggga
     1561 ctgtacgcca gaaccgccag gacagctggt ttcgtaaaac agcttcgaag atgattaacc
     1621 ggcttattca gcgcaccacc ggcaaagcga tgggtgacta cggttgtatg ctgcgcgcct
     1681 atcgccgtca tattgtcgat gcgatgttgc actgccatga acgcagcacc tttatcccga
     1741 ttctggcgaa tatcttcgcc cgccgtgcca ttgaaattcc agtgcatcat gccgagcgtg
     1801 agtatggtga atccaaatac agctttatgc gcctgattaa tttgatgtac gacctggtga
     1861 cctgccttac cacaacgccg ctacgtatgc tgagtctgct cggcagcatt attgcgattg
     1921 gaggttttag cattgcggtg ctgctggtga ttttacgcct gaccttcgga ccacaatggg
     1981 cggcagaagg cgtctttatg ctatttgccg tgctgtttac ttttattggc gctcagttta
     2041 tcggcatggg attactcggt gaatatatcg gcaggatcta caccgatgtc cgcgcccgcc
     2101 cccgctattt tgttcagcaa gttatccgtc catccagcaa ggaaaatgaa taatgaaaac
     2161 cgtcgttttt gcctaccacg atatgggatg cctcggtatt gaagccctgc tggctgccgg
     2221 ttacgaaatt agcgccattt ttacccatac cgataatccc ggtgaaaaag ccttttatgg
     2281 ttcggtggct cgtctggcgg cggaaagagg cattccggtt tatgcgccgg ataacgttaa
     2341 tcatccgctg tgggtggaac gcattgccca actctcacca gaggtgattt tctcttttta
     2401 ttatcgccat cttatttgcg acgagatttt gcagctcgct cccgcaggtg catttaatct
     2461 gcacggttcg ctgttaccaa aatatcgtgg tcgcgcgccg ctgaactggg tgctggtgaa
     2521 cggtgaaaca gaaactggcg ttacattgca ccgaatggta aaacgtgccg atgccggggc
     2581 cattgtggcg caactgcgcg ttgccattgc gccagacgat atcgctatta cgctgcatca
     2641 taaattgtgc catgccgcgc gccagctact ggaacagaca ttacccgcca ttaaacacgg
     2701 taatattctg gaaatcgccc agcgcgaaaa cgaagccacc tgttttggtc gcagaacgcc
     2761 ggatgacagt ttcctcgaat ggcataagcc ggcatccgta ctgcacaaca tggtacgtgc
     2821 cgttgccgat ccgtggccgg gtgccttcag ctatgttggc aatcagaaat tcaccgtctg
     2881 gtcgtcgcgt gttcatcctc atgccagcaa agcacagccg gggagcgtga tttctgttgc
     2941 gccactgctg attgcctgtg gcgatggcgc gctggaaatc gtcaccggac aagcgggcga
     3001 cggcattact atgcagggct cgcaattagc gcagacgctg ggcctggtgc aaggttcacg
     3061 cttgaatagc cagcctgcct gcaccgcccg acgccgtacc cgggtactca tcctcggggt
     3121 gaatggcttt attggcaacc atctgacaga acgcctgctg cgcgaagatc attatgaagt
     3181 ttacggtctg gatattggca gcgatgcgat aagccgtttt ctgaatcatc cgcattttca
     3241 ctttgtcgaa ggcgatatca gtattcattc cgaatggatt gagtatcacg tcaaaaaatg
     3301 tgatgtcgtc ttgccgctgg tggcgatagc cacgccgatt gaatataccc gcaacccgct
     3361 gcgcgtattt gaactcgatt ttgaagagaa tctgcgcatt atccgctact gcgtgaagta
     3421 ccgtaagcga atcatcttcc cgtcaacttc agaagtttat gggatgtgta gcgataaata
     3481 cttcgatgag gaccattcta atttaatcgt cggcccggtg aataaaccac gctggattta
     3541 ttcggtatca aaacaattac ttgatcgggt gatctgggcc tatggcgaaa aagagggttt
     3601 acagttcacc ctcttccgcc cgtttaactg gatgggacca cgactggata accttaatgc
     3661 ggcacgaatc ggcagctccc gcgcgattac gcaactcatt ctcaatctgg tagaaggttc
     3721 accgattaag ctgattgatg gcggaaaaca aaaacgctgc tttactgata ttcgcgatgg
     3781 tatcgaggcg ttataccgca ttatcgaaaa tgcgggaaat cgctgcgacg gcgaaattat
     3841 caacattggc aatcctgaga acgaagcgag cattgaggaa ctgggcgaga tgctgctggc
     3901 gagcttcgaa aaacatccgc tgcgccatca tttcccaccg tttgcgggct ttcgcgttgt
     3961 cgaaagtagc agctactacg gcaaaggata tcaggacgta gagcatcgta aaccgagcat
     4021 ccgcaatgcc caccactgcc tggactggga gccgaaaatt gatatgcagg aaaccatcga
     4081 cgaaacgctg gatttcttcc tgcgcaccgt tgatcttacg gataaaccat catgaccaaa
     4141 gtaggcttac gcattgatgt cgataccttt cgtggcaccc gtgaaggcgt gccgcgtctg
     4201 ctggaaatct tgagtaagca taatattcag gccagcattt ttttcagcgt cggcccggac
     4261 aatatgggcc gccatctctg gcgactggtg aagccacagt ttttgtggaa gatgctgcgc
     4321 tcaaacgcgg catcgcttta tggctgggat attttactgg caggtacggc ctggccaggt
     4381 aaagagattg gtcatgccaa tgccgatatc attcgtgaag cggctaaata tcacgaagtc
     4441 ggcctgcacg cctgggatca ccatgcctgg caagcccgta gcggtaactg ggatcggcaa
     4501 acaatgatcg acgatattgc acgcggcctt cgcactctgg aagagattat cggtcaaccg
     4561 gtaacctgtt ctgccgctgc gggctggcgt gccgaccagc aggtgatcga agcaaaagaa
     4621 gcgttccatt tgcgctacaa cagcgattgt cgtggggcca tgccgttccg tccactgctc
     4681 gaatcaggaa accctggcac tgcgcaaatt ccggtgacct tacccacctg ggatgaagtg
     4741 attggtcggg atgtgaaagc agaagatttt aacggttggt tactcaaccg catcctgcga
     4801 gataaaggca cgccggttta taccattcat gcagaagttg aaggctgcgc ttatcagcat
     4861 aattttgtgg atctcctcaa acgcgcagct caggaaggcg tgacattttg ccctttaagc
     4921 gaactgttat cagagacgtt gccgctcgga caagttgttc gcggaaatat tgccggacgt
     4981 gaaggctggc tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg gtacgttacc
     5041 ttatcggcct cttcgcgttt attgcctgct attacctgtt accgatcagc acgcgtctgc
     5101 tatggcaacc cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg gcatccggcg
     5161 actggattgt gccccatctg ttagggctac gttattttga aaaacccatt gccggatact
     5221 ggattaacag cattgggcaa tggctatttg gcgcgaataa ctttggtgtg cgggcaggcg
     5281 ttatctttgc gaccctgtta actgccgcgc tggtgacctg gtttactctg cgcttatggc
     5341 gcgataaacg tctggctcta ctcgccacag taatttatct ctcattgttt attgtctatg
     5401 ccatcggcac ttatgccgtg ctcgatccgt ttattgcctt ctggctggtg gcgggaatgt
     5461 gcagcttctg gctggcaatg caggcacaga cgtggaaagg caaaagcgca ggatttttac
     5521 tgctgggaat cacctgcggc atgggggtga tgaccaaagg ttttctcgcc cttgccgtgc
     5581 cggtattaag cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat ctctttattt
     5641 acggctggct ggcggtcatc agttgcgtac tgacggttct cccctgggga ctgacgatag
     5701 cgcagcggga gcctgacttc tggcactatt ttttctgggt tgagcatatt caacgctttg
     5761 cactggatga tgcccaacat agagctccgt tctggtacta cgtgccggtc atcattgccg
     5821 gtagtctgcc gtggctggga ttactccccg gtgcactata cacaggctgg aaaaaccgca
     5881 agcattccgc aaccgtctat ttgttgagct ggacgataat gccgctgctg tttttctccg
     5941 ttgctaaggg taaattgccc acctatattc tttcctgctt tgcacctttg gcaatgctga
     6001 tggcgcatta cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg attaatggct
     6061 ggattaacat cgcttttggc gtcactggca ttattgccac atttgtggtc tccccctggg
     6121 gaccaatgaa cacgccggtg tggcaaacct tcgagagcta taaagtcttt tgtgcctggt
     6181 cgattttttc gctatgggca tttttcggct ggtacacctt aacaaacgtc gaaaagacct
     6241 ggtcttttgc cgcgctttgc ccgctggggc tggcgttgct ggtaggattt tcaattcctg
     6301 acagagtcat ggaaggaaaa catccgcaat tttttgtcga gatgacacaa gaatcacttc
     6361 agccaagccg ctatattctt actgatagcg tcggtgttgc cgcaggtctg gcatggagtc
     6421 tgcaacgcga tgacatcatc atgtatcgcc agacaggtga gttgaaatac ggccttaatt
     6481 atccggatgc gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg cttaatcaac
     6541 atcgtcagga ggggattatt acactcgtgc tttcggttga ccgcgatgaa gatatcaaca
     6601 gtctcgccat tccgcccgca gatgccatcg atcgtcagga gcgtctggtg ctgattcagt
     6661 atcgtcccaa atgatctggc taacattagt ctttgccagc ttgcttagcg ttgccgggca
     6721 gttgtgtcag aaacaggcaa cctgctttgt ggcgataagc aaacggcgca aacatatcgt
     6781 gctgtggctg ggactggcgc tggcttgtct tggtcttgcc atggtgctct ggctgctggt
     6841 cttgcagaac gtaccggtag gtattgccta cccgatgtta agtctgaatt ttgtctgggt
     6901 gacgctggct gcagtaaaac tgtggcacga accggtatcg ccgcgtcact ggtgtggggt
     6961 ggcgttcatt attggtggca ttgtgatcct cgggagtacg gtgtaatggg cctgatatgg
     7021 ggattattca gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt tgcggcgagt
     7081 catctgccgc cgatgacgca cttatgggat tttatcgcgg cgctgttagc ttttgggctg
     7141 gatgcccgga tactgttact cgggctgctg ggttatctgc tttccgtttt ttgttggtat
     7201 aaaacactac ataaacttgc gctaagcaaa gcctatgcct tgctcagtat gagttatgtg
     7261 ctggtatgga ttgcttcaat ggtacttccc ggctgggaag ggactttttc gctgaaagca
     7321 ctactgggag tagcctgtat tatgagcggg ttgatgctga ttttcctgcc cacgacaaaa
     7381 caacgttact gagttttccc tgccacttta cactgccgtt cccactcatc ggggctataa
     7441 cagcttgcag agaggacttt caccgtatgc agcttttcac gattaataca ataaagcgca
     7501 ttctgcaaag gcgagagtaa atcccccact ttcacggcga agtcactctt cacttcggcg
     7561 atcattttta tcgcgccgcc agcatcgcag agcataagta catgtctgtt atcttgttta
     7621 ttgcagcacg attttttgac cagccattcc attgtattat cctgtttgct aagagttttc
     7681 atatcaattt acagattaac agataaactc tttatcaacg gcaatggaat ggcagaactg
     7741 agaagtgaaa cggagggtta attattgctg acgttgcacc cactctttta gcgcctgacg
     7801 tgaaatttta ataccgccgt ttttcagctc cggcggcaaa gtcagccaac gtaccggttg
     7861 ctgaaaacgg gccagcttat ctttcaccca ttcactaaga tcaacgctct cgtggtcata
     7921 ctccatcacc gccaccggtc gatgaccaaa ctccttgtcg gcaacgggga cgataaacac
     7981 ctgcaaaacc gcaggatgtg cagcaattac gcgctcgact tcttccgtct gaataccctc
     8041 tccgccactg aagaatagat tgtctaaacg tccgacaatg gtcagcttgc cattatgcat
     8101 ctcaccgcga tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa ccagttgccc
     8161 gttacgccag taaccttctg ccatactggc agcccgcagc cacacttcat tattaacgat
     8221 tttcacttcc cgacccggca gcggcgaacc aacgtctgcc aggccgtcgg cttctttcgc
     8281 acacaccgtg gaggcaaact cggtcagacc atagccgcaa aagcaacgaa tcccctgctc
     8341 gcgcgcctgt tccgtcaact cgaccgggat agccgcgccg ccaagtaaca ccgctttcag
     8401 ggaaacggaa ctgcggttaa ccagcaaacg ccagagttgt gttggcacca gtgaagcgtg
     8461 agtacagcct gccagcattt gctccaatgg ctgtttatca cgcaccgtca tccgcgcacc
     8521 agcgtataac cagcgccaca taattccctg accggagacg tgaaacagcg gtaaagagag
     8581 caaccagtca tcgtgatcgc caaacggaat cagcgataac accccttcgg cactggcaag
     8641 atgggcctga taagtatgga cagcggcttt cggcaacccg gtagaacccg aggtcaacgt
     8701 cattgagcac agacgcgtcg gctgccacgt agcgacatgt gcgccttcaa cccgctgaat
     8761 gtgcagcgac gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg caaattgcag
     8821 cgtcagattg ggcagcaatt cttcaagcaa cggttgcggc agctgagggt tcacgggcaa
     8881 cacccgcgcc ccgcattgca gtaacgccag ccaagcgagc agcgtttgcg gcgtattcca
     8941 cgcccgcaac atcacgccgc tgtcctcaac cacaccctgc accgcaaatc cggaggctaa
     9001 ttcatcgacg cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa gacgtaaggc
     9061 gatggcttct ccccgcactt gccgccagtg acgccacggc cagtcagaga agatcataac
     9121 aaccgctcca gtgcatcaac ttccacgaca ggcagcgtgc tacccggcca gcgacgtacc
     9181 tgctgcgcct gcattagatc cagcgtgtcc agccctggaa tggtgtccgg cgttaaccag
     9241 gcagcaatcc gcgccagttg cgttaagcct aagctcgatt caatggaaga actgatcact
     9301 gccgtcagcc ccagcgcgtg cgccgcctgt acctgctcgc gaactttctc cagactgccc
     9361 gtgagcgtgg gtttgataac taccgcgcgc acgccctctt cagccacaaa ggcaaaatcc
     9421 ggctcgcgca agctttcatc ccaggcaatg gcaatgccgg tttcacgggc aaacgctcgc
     9481 gaatcatcgc gggttttgca cggctcttcg agaaacgcga tgcggtggcg ataatccggg
     9541 ttaacgtatt tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt tgcgtcaaga
     9601 cgcaaatgca gatccggaat tgcctccagc aacagattca ccaccatgcc gtcgcgcacc
     9661 gcttcgtaca atcccacttt gaccttcgcc actttctcgc ctggcatatc tgcaagtttg
     9721 aggatcagat cgtccggatc gccattacac agtggtgccg tacggtaatt ggccgcctgc
     9781 gggagcgtat ctgccagctc cgccaatgca caacttacgc caaaggcaac ggaaggcatc
     9841 tgcggtaatt cgcaatcgcc tgccagccag ttatttaccc aggcaagcag cacactttgc
     9901 gcctcttccc aggtttcctg actgaagccc ggcagtgggg agatctcccc ccacccttcg
     9961 cgctcgccct cacgcaggca aacgtacagc ccgtcgcggg tttttaaccg cctgtcgcgc
    10021 agaaccaccc ccgcgtccat ggggatctgc cagcggtata cctgcgcgct acgcattacg
    10081 gattccgttt gaatttgctg aagtcaggct gacgtttctg gttgaaggcg ttgcgacctt
    10141 cctgaccttc ttccgtcatg tagaacagca tggtggcgtt gcccgccagc tcctgcagac
    10201 ctgcctgacc gtcacagtcg gcgttcagtg cagctttcag gcagcgcagc gccatcggac
    10261 tgttttgcag catttcacgg caccagcgaa cggtttcttt ttccagatcc gccagcggta
    10321 ccacggtgtt gacaaggccc atatccagcg cctgttttgc gtcgtactga cggcacagga
    10381 accagatttc acgcgctttt ttctgcccga cgatacgagc catgtaggaa gcgccccagc
    10441 cgccgtcgaa ggaaccgact ttcgggccag tctgaccgaa gatggcatta tctgccgcga
    10501 tagtcaggtc gcacatcatg tgcagaacgt gaccaccgcc gatggaatag ccagccacca
    10561 tcgcgacaac cggtttcgga caggtacgga tctgacgctg gaagtccagc acgttcaggt
    10621 gatgtacgcc ggaatcatct ttatagccgc cgtaatcacc acgcactttc tggtcaccac
    10681 cggagcagaa cgctttatcg cctgcgccag tcagaatgat cacgccgacg ttatcgtcat
    10741 aacgcgcatc tgccagtgcc tggatcatct cttttaccgt cagaggacgg aaggcattgc
    10801 gcacctgcgg acgattaatg gtgatttttg cgataccgtc ggtggatttt tcataacgaa
    10861 tgtcctcaaa gccttcggag cagtcgtgcc attcaaccgg tgcgtaaagc attgcttcat
    10921 caggataaat catattgtgt cctttagtca gaaacgcaag atctgcgcca gacttgcgat
    10981 tacgccagcg ggattttccc gatgcgcgtt atgtccggcg cgaggaatga catggcagtc
    11041 ggcagccagt tccgccgcca gggcgcggaa tttgctgtca cgttcaccac ataaataata
    11101 aaacgcaaat gtgcgggcgc taaggttagc acgtaaatca ggctggacgg cgagagaagt
    11161 cgcctccagc atggcggcaa gggttgcgcc attattgttg ctgcgcagca ccaccagctc
    11221 ccggcgttga tcgtcattga gcgaggcaaa aacaggctgt tgataccagt cggcaaatac
    11281 cgcggttaat ggttcggaac gaaaacgctg cgcccattgg cgatcggaac gctgacgttc
    11341 cgcgcgttgt tcagcatttt gcagccccgg atgaccgcct tcgacaacaa ccccacaaag
    11401 ccccgccagc ccctggcaag cggccatcat cgccacccgt ccgccaagcg agtaccccac
    11461 cagccagaag ctaaggatgt tgtaactaac cagggtttta cgcagtaagt cggtgacatc
    11521 atcaaatcca tcgacgctaa tcgccgccga accaccgtga cctgggagat caacatacaa
    11581 ccgtgagtag tcggcaaacg cctcgccgac ttcctgccat tcgaggcaat cgccggaaaa
    11641 accgtgcaaa aacaccagcc agggtaaacc tggtttgccg tgttttgcct gcgcgtgcag
    11701 gatcataaat ggcttacctg cgccagcagc tgctggagcg tttgcgcgcc gtcggtgtcg
    11761 ttaaccacca tttcaattac cgtggtggtc ggagtacgcc aggcgtcggc aagtaccgtt
    11821 tcaagttcct gccagttttg cggacgatga tatttcagct caaacatcgc ggcagcgtgc
    11881 tcaaaatgga cgttttgcgg catcagatag aaacgctcgc gctcgctttt cggcgttggc
    11941 agcagcgaga aaatttgccc gccgttgttg ttcaccacaa ttaataccag cggcgcggaa
    12001 acctggcgca ataacgccag cgcattcagg tcgtaaagtg cggagagatc gcccacaatc
    12061 gccagcgtcg gtttgccgct tgcccgctga acgccggcgg cagtcgagag cagcccgtcg
    12121 ataccgctgg ccccacggtt gctgtacacc gggtaacctg ccggaagttg cgaaagcgca
    12181 tcaatcagac gtaccaccag gctgttaccg acaaacaatt gcccctgttc aggcagatag
    12241 tcgctgatgc gatgcgccag ttgcgcttcg ccaaacgcat cgcgacaggc aataaccgcc
    12301 tgcattgcct gttccgccag gcgcgggatt tcaacgcacc agggctggcg tttttctgcc
    12361 ggatgcagtt ccagccagtc ggcaatattg gcaattaagc ggcgtccacg atggtgtgcc
    12421 ggatcaagcc gcccttcgat gtcatccaca atccagtact cttcgggttc acagcttgcc
    12481 tgccattgca ggagccgttt gcccgtcagg ctgcttccca gttgcaccac aatttgcgcc
    12541 tgttgcagtt cgctggtcgc tttggcattg cctaaccaga gatcggcaca cggcagcggc
    12601 tgtccggttt gcgacagcac gtcgccaatc agcggccagc caagagtttg cgcccacagg
    12661 gcaacttttt tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc gcgcttttgc
    12721 cgccagaaga accagtcgcg ctgtttttcg ctctcaagac gaggcgcttc acgcagccac
    12781 ggcttatcat cctgccacca gtcacccaga cgctgttgcc agctaagccc agtgtcgtcc
    12841 atctcgccat acagcggttc agcaaacggg cagttgatat ggaccccacc agcatgaagc
    12901 gtaccgagag catggtcgat ggtagaaacc agccaacgtg cggggatatc ctgggtcggg
    12961 cgcggcagtg aaatactgtg cgtggggtga gaggcgaaca ttcccggctg acgaatcgcc
    13021 tgattcgcgc cgcagtcaat tagctccggc gggcgatcgg cggttaacag gatcagtttt
    13081 tctccggtta acccagcttc aatcagtgcc ggatagagat ttgctaccgc cgtgccggag
    13141 gtcacaatca ccgccaccgg ctgcttgctg actttcgcca gccccagcgc cagatgcccc
    13201 agtccacgct catcgaaatg ggtgtggtga atgaatgcgg aattctccgc cgccgctaac
    13261 gttaacggtg tagaacgcga gccaggggcg atgcagatgt gtcttacgcc gtgacgcgtt
    13321 aatgcttcca gaatgaccgc cgcccagcgt cggttaaatg cgcttactga catgagtttg
    13381 tccggtatca atattgcggc taagtataag gagctacaaa aatagattat tgatatgaat
    13441 cggtaatgat gcgactcatt actattccat ttgtaataaa gtacgcagcc ctgccgcttt
    13501 gttgtcgatt tcctgccact cttgctcggg gtcggaacca cggacaatgc ccgcgccagc
    13561 atatagccgc acgacattgc cgctgatttt tgctgagcgc agggaaacgc agaattcgct
    13621 ttgttgtaat gagagatagc ccgcagaacc ggcgtaccat tctcgggtga acggttcgtg
    13681 atgggcgata aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg ccgttggctg
    13741 caactgatgt aaacagatca catcatccgc tttgttgagt gaagtccaga tacagcggcg
    13801 aagatgctgc actttacgca gacgcagtac ctgcggcggt aaaacatcca gcgtctgggt
    13861 atcggcctgt aatcgttggc agatatcttc caccaccagc atgttctcgc gctggttttt
    13921 atcatccgcc atcagccact ctcctaactg ctgcgcctgc ttatcatcag gatgatttgc
    13981 tactgttccc gccagcgctt cagtacgcag cgctttgtca cgccgccgcc ataaccgttc
    14041 cggtgaagag ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat ggtagcaatt
    14101 gagattcagt cgacgactgg cagccatcat cgccgccgca ttgaccggac ttgcgaaatg
    14161 caggtcagtt gcccgagcaa gcaccacttt gtcgagctca ccttcggcga tggttttcgt
    14221 tgccagttcg attaattgcg tccagcccgt tttgtccggc cagtgttgtt ctcgcgtagt
    14281 ggttaaatgt aacccaggca agggcttgat actcaccagt gtggcgataa attctcttgc
    14341 cttaatcgca tcgtgctgaa gggagctttc gctgaatagc gtcagccgca gcgtggcttt
    14401 accgccacag cgtcgccatt ccaggcgtgg taaaagtaaa ttgccctgcg acgggtcaaa
    14461 cgcattcagc ccccaaatgc gtaagtcggc gtgttccggg tgctggcgaa gaaaacgttg
    14521 tgcctggtcc aacgacgtaa aacgggtaat cgcgcccagg acggcagctt cttcatcacc
    14581 attacgttgt tgccagtaga attgcgggta cgtttgctga ctggccagcc agctcaaggc
    14641 atcaaaagcg tctttgagag ggaaaggaat atcgataacc cgaatgccgg gtgtcgccgg
    14701 aatctcatgc gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg attgcacgcg
    14761 gacctctccc tgttaaaagc ctcacattat acggggtact acaaaaaaaa tgcagtaccc
    14821 cggtgtaggg aggttttaac ggcgtgccag caacagtcct agtaccagcc caacggccgc
    14881 acccacacca attccctgcc agggtttttc gtggacgtag tcatcagcac gataaacagc
    14941 ctgcttcgcc cgatagtaat aactgtctga agcctggcta acccgttttt tcacatcatc
    15001 cagcgctttt tctgcacgcg ctttcagctc aacatatttc tgatcggcgg gatcgccaga
    15061 ggagcggagc acctcttcca gtgtttcact aagcagcgtc aggtcgtcat cgatacgtgt
    15121 atcaccaaac tgattagaca ttctcgttct ccatttgcgt aaaacctgtc actaactata
    15181 gacaagggtt attgattacg cctgaattac ctcgcgtgcc atcccaatgt gtgggatacc
    15241 atcttcttca tagatctccg tcacagggat aaaaccgaaa ctctggtaga agttttgcag
    15301 atgcgcctgc gccccgaggt agacaggctt atcaggccag tgatgcgtgc aagtgtcgag
    15361 ggttttactc atcagttgct gccccacttt ttcgccacgc aacgcttcac tgacaatcac
    15421 ccgaccaata acgaccggct caagatcatc atcacttttc agaatcctcg catatgccac
    15481 cagttcatca tttttccaac cgagaatatg gcgattatcc cccgtcaaat catcaccatc
    15541 gatatcctga taagggcaat tttgctcgac aacaaacacc gcgcaacgca gttgtaataa
    15601 ggcataaagc tgggaaaccg ataattcaga gtgatgcaga tcttgccatt caatcataat
    15661 gctctccttt tagatgtatg ccttatacta gccggaatat tttttgaaac gtgatgaact
    15721 catggaatta atttttttag gtacttcggc cggtgtgcca acccgcacgc gcaatgtcac
    15781 ggcaatattg ctaaacctgc aacatccgac ccagagcgga ctttggttgt ttgactgcgg
    15841 tgaaggcacc cagcatcagc tactgcatac cgcctttaac cctggaaaac tggacaagat
    15901 ttttatcagt caccttcatg gcgatcatct ttttggttta cccggcttgc tgtgcagtcg
    15961 ttctatgtca ggcattatcc aacccttaac gatttatggt ccgcaaggta tccgtgaatt
    16021 tgtggagacc gcgctgcgga ttagcggctc atggaccgat tatccgctgg aaattgtcga
    16081 aattggcgct ggcgaaattc tcgatgatgg cctgcgcaaa gtgaccgctt atccaatgga
    16141 acacccactg gaatgctatg gctatcgtat tgaagaacat gataaaccgg gtgcattaaa
    16201 tgcccaggca ttaaaagctg ctggcgtacc gcctagccca ctgtttcagg aattaaaagc
    16261 gggcaaaaca atcatgctgg aagatggaag gcagattaac ggcgcagatt acttagctgc
    16321 tccagtgcca ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg atgccgcact
    16381 tgacctggct aaaggtgtcg atgtcatggt gcacgaagcg acgctggata taaccatgga
    16441 agccaaagcc aatagtcgcg gtcatagctc tacacgccag gctgcgacac tggcccgtga
    16501 ggctggagtc ggtaagctaa tcattaccca cgtcagctcg cgctatgatg acaaaggttg
    16561 tcagcacctg ttacgtgaat gcaggtcaat tttcccggcg actgaactag cgaatgattt
    16621 caccgtgttt aacgtttaac tctgcttatt attaaaaaca gggcgaaact tgccctgtta
    16681 tcgcaacccg cgctacatat atccactcac acgggcatat tgagcgattt ttgatattta
    16741 ttcatctcct tacgcactat attcagagca ataagttgcc tctttttatc ttcattcagc
    16801 acaacctttg caactaaaag gagcatatga tggttacagt tgtcagcaat tattgtcaat
    16861 tatctcaaac ccaactcagt cagacatttg cagaaaaatt tactgtgacc gaggaattac
    16921 tgcagtcttt aaaaaaaaca gcgttatccg gagatgaaga aagcattgag ttactgcata
    16981 atattgcgtt aggttatgat gaatttggga aaaaagctga agatattctt taccatattg
    17041 ttagaaaccc aacaaatgat accctatcga ttatcaagct tataaaaaat gcctgtttaa
    17101 aattatataa tttggcacat accgcaacca aacaccccct caaatcacat gattcagata
    17161 atctcctgtt caaaaaacta ttctcccctt cgaaattaat ggcaattatc ggtgaggaca
    17221 ttcctctcat atcagaaaaa cagtcacttt caaaggtact tttaaatgat aagaataatg
    17281 aactgagcga tgggacaaac ttctgggata aaaatcgtca attaaccaca gatgaaatag
    17341 cttgctatct taaaaagatc gccgccaatg caaaaaatac tcaagtcaat tatcctactg
    17401 atttctacct ccccaattcc aacagcactt acctggaagt cgctctcaat gataatatta
    17461 agagcgatcc atcatggccg aaagaagtcc agttattccc cataaatact ggcggacact
    17521 ggatattagt ttcgctacag aaaatagtta atgaaaaaaa caacacacaa caaataaaat
    17581 gcatcatatt caattcatta cgtgcactag gccatgaaaa agaaaattca cttaagcgta
    17641 tcattaacag tttcaatagt ttcaattgtg accccacgag agaaacgccg aataataaga
    17701 atataacaga tcatttaact gaaccagaga taatattttt acatgccgat cttcagcaat
    17761 acttaagcca aagttgcggt gcatttgtgt gcatggcagc ccaggaagtg attgaacaaa
    17821 tggaaagcaa ttctgacagc gccccctata cgttattaaa aaactatgct gacagattta
    17881 aaaaatattc agcagaagag cagtacgaaa ttgattttca acatcgtctg gaaaacagaa
    17941 attgttattt agataaatat ggcgatgcaa atatcaatca ttattataga aacttagaaa
    18001 taaaaaactc acaccccaaa aatagagcat caagcaaaag agtaagttaa catcattgtt
    18061 aattaattcg accagtcagc aaatatgctg actggtcgaa catagtttgc taaaaagtca
    18121 tcactgactg atgtcagtca caccacccgc cagttcaatc ttcgggtatt tccagcgtat
    18181 ctcggcacgc gattgctggt ctgttgatgt agtggattgt tgttgctgac ttttcttacc
    18241 ctcagattt
//
LOCUS       DAZFBR010000095        18066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFBR010000095 DAZFBR010000000
VERSION     DAZFBR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.095"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            1..571
                     /gene="ykgH"
                     /locus_tag="ACHJZ2_003573"
                     /pseudo
     CDS             1..571
                     /gene="ykgH"
                     /locus_tag="ACHJZ2_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(814..1509)
                     /gene="ykgG"
                     /locus_tag="ACHJZ2_003574"
     CDS             complement(814..1509)
                     /gene="ykgG"
                     /locus_tag="ACHJZ2_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO4569616.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAKNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1502..2929)
                     /gene="ykgF"
                     /locus_tag="ACHJZ2_003575"
     CDS             complement(1502..2929)
                     /gene="ykgF"
                     /locus_tag="ACHJZ2_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO4569617.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGLWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2940..3659)
                     /gene="ykgE"
                     /locus_tag="ACHJZ2_003576"
     CDS             complement(2940..3659)
                     /gene="ykgE"
                     /locus_tag="ACHJZ2_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO4569618.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4187..5041)
                     /gene="rclR"
                     /locus_tag="ACHJZ2_003577"
     CDS             complement(4187..5041)
                     /gene="rclR"
                     /locus_tag="ACHJZ2_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO4569619.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWSVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5267..6592
                     /gene="rclA"
                     /locus_tag="ACHJZ2_003578"
     CDS             5267..6592
                     /gene="rclA"
                     /locus_tag="ACHJZ2_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO4569620.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6701..6937
                     /gene="rclB"
                     /locus_tag="ACHJZ2_003579"
     CDS             6701..6937
                     /gene="rclB"
                     /locus_tag="ACHJZ2_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO4569621.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6949..7542
                     /gene="rclC"
                     /locus_tag="ACHJZ2_003580"
     CDS             6949..7542
                     /gene="rclC"
                     /locus_tag="ACHJZ2_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO4569622.1"
                     /translation="MEKYLHLLSRGDKIGLALIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQGSNESSSTLKTEY"
     gene            complement(7702..8571)
                     /locus_tag="ACHJZ2_003581"
     CDS             complement(7702..8571)
                     /locus_tag="ACHJZ2_003581"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO4569623.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            8820..9677
                     /locus_tag="ACHJZ2_003582"
     CDS             8820..9677
                     /locus_tag="ACHJZ2_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4569624.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIAISLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(9798..14051)
                     /gene="fdeC"
                     /locus_tag="ACHJZ2_003583"
     CDS             complement(9798..14051)
                     /gene="fdeC"
                     /locus_tag="ACHJZ2_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO4569625.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSAAVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDTYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGNAKQTYKVTVTDANNNLLKDSEVTLTASPANLVLTPNGTAKTNEQGQA
                     IFTATTTVAAKYTLTAKVSQADGQESTKTAESKFVADDKNAVLTASSDVTSLVADGIS
                     TAKLEVTLMSANNPVGGNMWVDIKTPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMN"
     gene            14617..15177
                     /locus_tag="ACHJZ2_003584"
                     /pseudo
     CDS             14617..15177
                     /locus_tag="ACHJZ2_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            15385..15741
                     /locus_tag="ACHJZ2_003585"
     CDS             15385..15741
                     /locus_tag="ACHJZ2_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569626.1"
                     /translation="MNKNLILAFALFSLPVFAEEDLGPGKYVCDIRISSLDTATQILS
                     KSATVLDNGNNFIVQMPNGDQLYSPDLENVDDGIKQKATIGGVTFIRRPTFNDRFIVE
                     DGNTGFFYKMRNCEKK"
     gene            complement(16029..16955)
                     /locus_tag="ACHJZ2_003586"
     CDS             complement(16029..16955)
                     /locus_tag="ACHJZ2_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4569627.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKS
                     LEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            17112..18032
                     /locus_tag="ACHJZ2_003587"
     CDS             17112..18032
                     /locus_tag="ACHJZ2_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4569628.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT         4827 a         4439 c         3913 g         4887 t
ORIGIN      
        1 ggcaatggtg cttttatttc tgtatatcaa taaagataat ataaaagtga gttacagcct
       61 aaaaataaat cagacaacac ccggcatact tgttaactgt gatagcaata ataactttgc
      121 ctgtcagaca acaatgactg aagatgttat tcagcgaatt actacatttt ttcacaccag
      181 cccagatgtc aagaacagag aaataaagct ggaatggtca ggagataaga gagatttacc
      241 aactgctgaa gcggaaatat ctcgcgtgca ggcgtctatt atcaaatggt atgcgtcaga
      301 ataccataat ggcaggtaag ttctcgatga gatacaaacg ccttcagcaa ttaacagtga
      361 gctttataca aaaatgatat acctgaccag gaactggtca ttgtatccga acggtgatgg
      421 ctgtgtaact ataagctcac cagaaataaa aaataaatac cctgctgcca tttgcctggc
      481 tctgggattt tttctaagca ttgtaatttc tgtaatgttt tgtcttgtca aaaaaatggt
      541 agatgaatac caacaaaact ctgggcagta ataaacagca ttctgataga gagtgcctgt
      601 ataaagctga agtgacaatg accgggaagg gaacccgttt tcaggaatgg atgcctgtct
      661 ttttcactgt aaatatcctg gcattagtaa tgccaggata tttacagaag aggagcataa
      721 ccatgacgga acggggttaa gtcaggttgt ccttaacgct attttcaacc tcgtatgcct
      781 tcttcaggtt tatgtccaga cttcatacct ctctcagcaa tcctcaataa tcagatacac
      841 cgctttcacc ggaccatgaa ccccgacgac tttgataagc tcaatgtccg ccgttgagct
      901 gggaccactg atgatgttaa tacatgacgg catgcgttca ccggcctgcg ctttctgatg
      961 caatctttct gcgagttgcg ctacacgcgg caggatagtg cttttacgca ggataaaaag
     1021 agatgattcc gggagcaggc tcaatgaacg cccacgctcg gcggcggaaa aaagaaccac
     1081 gcctcccgat tcggttaaac catattcagc atacacgaca cccactttag cctgctctgc
     1141 ctgcgagata ttcttggcac ctttcgccgg atcccaaaca acggcattgc attcctgctg
     1201 caaacgttcg ctaatcccta attcctccag cctcgtgtca ccgctaatca cgaccgactg
     1261 atctcccagc tctttacaca gacgtattgc agcttctgcc gccttcgcct cgctggtcag
     1321 ctcacagcgc gtcaacataa catcgctggc aaactgaata aacgcgtcac agcgctgctg
     1381 ttggttaagt tgggtaagcc gctcgttagc atagttatta agcggcgcat cttctgcttg
     1441 cggttcaagt cgcagcgggc gacccagtgc ctgagcaacg ttattcaaaa attcgcctcg
     1501 attatccatt ctttttctcc tgcgcctgat gtttcttaaa ccaactacgg aaactctctc
     1561 cgtcagcttc aggaagatcg cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg
     1621 gtgttttgcc gccattgata aaccagcttg ccgcatgagc accggccatc atcccgactt
     1681 tccacaatcc tggatgacta ttggcataag cgaacatttt tatcgcccgt tgctctgctt
     1741 ttgcggtgat ccctttttca gccatcaccc gacgatgacg caaaatcagt tttgacagcg
     1801 gaatacgcac cggacacacg ttgtcacaag ctgtgcataa agagcaggcg tagggtaaat
     1861 ctttaaaatc tttatagccg ccaagtagcg gagaaatcac cgcaccaatt ggccctggat
     1921 aaatagagcc atatccatga ccgccaatat ggcgatatgc cggacaagta ttcatacaag
     1981 ccccgcagcg aatacagcgc agcacatccc gaaattcaga ggccagcacc tcagaacgcc
     2041 cgttatcgac aataaccaga tgaaactctt caggaccatc aacgtgcccc gcttcgcgag
     2101 gtcctgtcag ccaggtgttg tatcccgtca aacgtgcacc aacggcactg cgcgccagca
     2161 tggtgatcaa tacatctacc tcggcaaacg tgggggcaat acgctccatt cccatcactg
     2221 caatatgcgt tttaggcagc gtggtacaca ttcgcgcatt accttcattg gtcaccaggc
     2281 ataccgaacc tgtctctgcc accgcgaaat tacagcccgt aatacctatt tcagcactga
     2341 ggaaatcttc gcggattttt tgccggatga ataaggtcat cgcttcaggc gtttccggcc
     2401 cctcatagcc cagacgttcg tgtagcactc gacggatctg atggcgatct ttatgaattg
     2461 ccgggaccac aacatgagag ggcggatctt gatccagctg gagaatatat tcacccaaat
     2521 cggtttcaat cacctgaatg ccagcatcct gcaacacatg attgacacca atctcttcgg
     2581 tcaccatcga tttagatttc accaccttcc gggcattttt gcgttgggca acctgtaaaa
     2641 tgtagcgggt agcgtcttct ttggtttttg caaaatagac gtgaccgccg ttttgcgtca
     2701 ctttttctga gagctggtac agataagcgt cgagattact cagaacatga tcacgtatct
     2761 gggcggcccg atcgcgccac tcctcccaga gccccaattc atcgaccatt ttttgccgat
     2821 ttgccccaat acgctgctgc gcgtttgcca ccgctttgcg catgatcgga tcttcaattt
     2881 gctgacggat gcgtgtctta aaatctgtat tactggtttt gatcgacatc tttatatcct
     2941 cagcggctca tcaacacttc agcaatatgc atcactttga ctttctgccc ttcccgttgt
     3001 aatcgcccac tgatgttcag caggcaactc acgtcagcac caattaaata ctcagggcgg
     3061 acttccatca ggtgcgcaac cttttctttc accatctcgc cggatatttc ggccattttg
     3121 accgagaacg tgccgccaaa tccgcagcag gtatcctgtt cagcaaaggt caacagctcc
     3181 agtccacgca catttttcag cagcgtaagt ggctcgtcct tcacccccag cttacgggcc
     3241 aggctacaag atgggtgata caccgctctc ccttgcaaac tggcacctac atcgactacc
     3301 cctaatttat taacaataaa agaggtgaga tcctgcatac gcgcggcaac cttttcggct
     3361 cgtgatgccc attcaggttc atccgccaga tacgtcgggt aactttttac ggcataggtg
     3421 caagagccag ccggtgaaat aatgggatcg tcgttatcct ccagtgcggc gatcagattt
     3481 ttcatccctg gaatcgcttc tttgatataa ccactattga tcgcaggctg accgcagcat
     3541 ccctgtttct ccgggaaatt tacgcgacag ccgagttttt ccagtagcag cacggagtct
     3601 cgtgccattc ttgatttcag ggcgtcacca atacaggtga caaagaaatt gacattcaca
     3661 actaatactc cattacttca tgcccattta ttcccgatgt ttccccccac ccggggatca
     3721 atcatcaata aatatggcgt tcttttttaa ttggctagca acataccacc ccttatttgt
     3781 atgacaaatg atagtctttc atccaaactt caaatgcaat aactaatttt atttctttcg
     3841 gcatcgctca cattttatgg ggaaatcgac gatcattttt ggcataagaa atccatttaa
     3901 aaatggctac atcccttata cctattcaga gctacttatt caggggttat tgagatatac
     3961 actgatggta tacaaaaaaa ttaccgacaa taatcacgta tcaagtaata gtcgggattt
     4021 tatttaattg aagcaattta gtttgcattt ttatttaaaa actgagctag agtccatcag
     4081 aaaaaaaata tataaagccc aaaccaccgt tggaaataga aatagttgaa aggctcacaa
     4141 ctcccggaat gcggattatc tttgccctga tttctggcgg ttttattcag ggtgcaagct
     4201 gtctgaccct ttcccgatat tctcccggag tacaaccaaa ctcgcggaca aacgccttgt
     4261 gaaaagatga ttcactggca tagcctactg actcagcgat caccacaaca gggagcattt
     4321 cccgggaaaa catctgggcc gctatttgta gacgcaactt tgttaataca gccagcggcg
     4381 tggttccgga aacatcacgg aaaagctggg caaaacttgc ccgggacatg tgggcgatgc
     4441 tggccagcga ttcgacgctc caggcgtgtc ctggcatttc cagcatttgc tgtattaccg
     4501 caccaagacg tggatgcaga agcaaactga gaatgttttt ctcagtatta acctgtgcaa
     4561 tccactcacg caccgcgagg gtaaagaatg tagcgcagat ctggctacac agtgcatcca
     4621 ctcccggcat tgccgatctg gattcctgtt gtaaaaacgg gatcgcctcc ctcagccagt
     4681 tatattccac gctgtggtta accggtgcta aaaacagcgt ttccggcaaa gacgttaaaa
     4741 aataacgtgc cgaatgttgc agccgaagag taccacagac aatacaggtc gattcattat
     4801 cgacatgact caggcgatga gctgaatttt gtggtagcag gaccacattt cccgggcgta
     4861 atgtaaaaat ctctcccgtc ggcatttcca gcttcgccgc tccttgcgtt aacgcatgcc
     4921 aacgaataac tgataattcc ccggcaccat gtggaagctg ccagtcactt cctaacacgc
     4981 aattcttatc gatcgttcct tgtggagcgt taagcatcaa caaacggcta agggcatcca
     5041 tatcagactc ctgagtggtt tttgattaca aacaccaaca atcttagtaa acaactaatc
     5101 taataccatc aaatagttaa tcatgatttt tgttttgtga ttctgattaa gaaaaagaaa
     5161 ataccagacg ataagacaaa atatcaagcg ttgtgaagaa atgttatttg ctcttttgcg
     5221 tctatagtca tgatgtcaaa tgaacgcgtt tcgacaggaa atcatcatga ataaatatca
     5281 ggcagtgatt attggttttg gcaaggctgg aaaaacatta gccgtcacgc tggcaaaagc
     5341 aggttggcga gtggctctca tcgaacaatc aaatgcaatg tatggcggga cctgtattaa
     5401 tatcggctgc atcccaacca aaacattggt tcatgacgca cagcagcaca cagattttgt
     5461 ccgagccata cagcgtaaaa atgaagtggt taatttttta cgtaataaga attttcataa
     5521 tcttgcagat atgcccaata tcgacgtgat caacggccag gcggagttta tcaataatca
     5581 tagcctgcgt gttcatcggc ctgagggaaa tctggaaatt catggcgaga aaatttttat
     5641 taataccggt gcacaaaccg tggttccgcc aattcctgga attaccacca cgccaggagt
     5701 atatgacagc accggattac ttaatctaaa agaattgcct gggcatttag gtattttggg
     5761 cggcggatat attggcgttg agttcgcctc tatgttcgct aattttggca gcaaggtaac
     5821 cattttagaa gcagcttcgc tgtttttgcc tcgggaagaa cgggatattg ctgataatat
     5881 cgcgacgatt ttacgcgatc agggcgtcga tattatcctc aatgcccatg tggagcgaat
     5941 cagtcaccat gaaaatcaag tgcaagtgca tagcgagcac gcccaactgg cggtggatgc
     6001 actgttaata gcttccggtc gtcaaccggc taccgcttcg ttacatccag aaaatgccgg
     6061 tatcgcagta aacgagcgcg gggcaattgt cgttgacaag cgattacata ccaccgcaga
     6121 caatatttgg gcgatgggag atgttaccgg cgggctgcaa tttacttaca tatcactgga
     6181 tgattaccgc attgtacgtg atgagttact gggtgaaggc aaacgtagta ctgatgatcg
     6241 gaaaaatgtg ccttattccg tatttatgac accgcccctg tccagggttg gtatgacaga
     6301 agaacaagcc agagagagtg gtgctgatat tcaggtggtg acattgcctg tagctgcaat
     6361 tccgcgcgcc agagtgatga acgatacccg tggggtatta aaagcgattg ttgataataa
     6421 aacccaacgt atattagggg catcactgct gtgtgttgac tcccacgaga tgattaatat
     6481 agtgaaaatg gtgatggatg ccgggctgcc ttatagcata ttacgcgatc agatatttac
     6541 tcatccgtcg atgagcgaat cactcaatga tctattttca ttagtcaaat aaactcaaaa
     6601 tcagacgcca gaacaaatat tctggcgtct cagagaaaag aatcttatta attccctgtt
     6661 actctataac ttcttaatca cttcattgat ggtattttat atgtttaaaa aatccgtttt
     6721 atttgcaaca ctattatctg gcgttatggc attttccacc aatgcagatg ataaaataat
     6781 tctgaaacat atcagcgtct cgtcagtatc agcatcaccg acagttctgg aggatgccat
     6841 tgctgatata gccagaaaat ataatgcttc atcctggaaa gtcacatcga tgcgaattga
     6901 taataattca accgcaacag cagtattgta taaataagga tattcacaat ggaaaaatac
     6961 ctgcacctgt taagtcgggg agataaaatt ggcctggcat tgattcgtct gagtattgcg
     7021 attgttttta tgtggattgg gttattaaag tttgtccctt acgaggcaga cagcattaca
     7081 ccattcgtcg caaacagtcc actaatgtcg ttcttttatg aacacccgga agactataaa
     7141 cagtatctga ctcacgaagg cgaatacaaa ccagaagcaa gggcatggca atcggccaat
     7201 aatacctatg gtttttccaa cggtcttggc gtcgtggagg tgattattgc tctgctggtt
     7261 ttggctaatc ctgtcaatcg ctggttaggt ttattgggag ggctgatggc atttaccaca
     7321 ccgctggtaa cactctcatt tttaatcacc accccggagg catgggtacc cgcattgggt
     7381 gacgctcatc atggtttccc ttatttatcc ggtgctggtc gtctggtatt gaaagatact
     7441 ctgatgctgg caggtgctgt catgataatg gcagattcgg cgcgggaaat tcttaaacaa
     7501 ggcagtaatg aatccagttc aacgttaaaa actgaatatt gataatccga acactgtctg
     7561 ttgttcactt tttcaggcgt caggccccct ttctgatgat gaagcctgac gccatattac
     7621 cggagatatg acagagtgaa gatcaatatc actcccctgt taaaagattc ttgtcgtctc
     7681 aggtaaaata catgattaat atcaatcatg tatttttaca gcaagacaac cacgaacaaa
     7741 ttcaggatta aaatgtttta ccgactcccc aacataaccc aaatcaagcg aactaatttg
     7801 tgccatttct ttctctgtca gtgagaaatc ccagatagca aaattttctt cgatacgctg
     7861 ttgatgtgtc gatttcggaa taacgaccac tccccgttga atattccaac gcagaatgac
     7921 ttgagaaatc gatttttgat gggcatctgc aatactctga agcagattat tttcaaaggg
     7981 tttatgtcgt ccaccaccta acggagccca tgcttcaggc tgcacgttat aatacttcat
     8041 ggttgccagc gcctctggtt gggcaaaata gggatgcaac tcgacctggt tgaccatcgg
     8101 tttaactctt accgtttcac aaaagttcgc cagaacatga ggatagaaat tggaaacccc
     8161 aatcgctttt aatttgccct cttcataggc atcttcgagt gcacgccacg cactgaaata
     8221 atcgcgcata gcctgatgca ataaatagag gtcgaagtat tctagtcccg atttttttaa
     8281 tgatgcttca atacctgctg ctgccgtatc ctgattcaac atatcctgca cccacaattt
     8341 agacgttata aataactctt ctcgggtaca taaaccttcg gatatagctt cacgaacggc
     8401 ttcaccgacg gcatcttcat taccgtatac cgctgcagtg tcgatgagtc gataccccgt
     8461 gcggatagcg ttcaacactg actgtttgca cacgttttta tcggtaacct gaaaaacacc
     8521 aaatcccatc atcggcattt tcagattgtt acttaatacg gaaaattcca ctgtcagtcc
     8581 ctccattagg catgaacaat gagtctacgt taaaacgtaa cctcaaatta gtatgtggat
     8641 tttgatatca cttatgcaaa aaattcatta ataatgtagg actgaaacct ctctattttc
     8701 ggggacaacg aagcagacgc taccagtgct tttgccttcg cccttgctgt ttttgataca
     8761 cttagggccc agggcataac gaaaatgtgc gatatgacaa agaattaacg gagaatgtga
     8821 tgatcaggca gaagattcta cagcagctcc tggagtggat tgagtgcaat cttgagcacc
     8881 ctatttcaat cgaagatatc gcacagaaat ctggctacag cagacgcaac atccagcttc
     8941 tgttccgaaa tttcatgcat gtgcctttgg gagaatacat tcgcaaacga aggctttgtc
     9001 gtgccgccat tcttgtccgg ctcaccgcga aatctatgct tgatattgca atctctttgc
     9061 attttgattc acagcagtca ttcagccgcg aatttaaaaa gttattcggc tgctctcccc
     9121 gtgtataccg ccaccgtgat tattgggatc tcgcaaatat cttcccttct tttttaatac
     9181 gtcaacagca aaagacggag tgtagattag tcaactttcc tgagacacct atttttggca
     9241 attcatttaa atatgatatt gaagtgtcga ataaatcacc ggatgaagaa gtcaaactgc
     9301 gacgtcatca tttagccaga tgtatgaaga attttaagac ggatatctat ttcgtctcca
     9361 cgtttgaacc gtcaacaaaa tcggtcgatt tactcacagt tgaaactttt gccggtacgg
     9421 tatgcaaaca aaccgattca gagatgccga aagagtggac aattaaccga ggactgtatg
     9481 cctctttccg ctatgaaggt gagtgggagc actaccctga atgggcgcga aacctctatc
     9541 tgatggaatt acccgccagg gggttagcca gagtgaacgg cagtgatatt gagcggtttt
     9601 attacaatga agatttcgta gaaaaggata gcaatgatgt tgtttgcgaa atatttatcc
     9661 ctgttcgtcc ggtttagctg tcgctatatc taaatgaact tataacgaga ctactgtcag
     9721 gcaattcaat aaataatggc gttgaagatg tcaacgccat tattgtttat tagaatgtta
     9781 cttccatatt cttaatatta gttcatcgcc tcctcgccct ttgccgtatc ggcatccacc
     9841 gcggtaatgg tcaccgtcac tggcttcatt tcgtacccgc catacgtcag ggcgttaaat
     9901 gtgaacgtat agacaccagg cttgctggta ctaaatgtac gcgtgatttt tccgctcacg
     9961 aaatggtcat ttttcgacgg caggaactga taatccttct ccgtcacccc ttctggcgtc
    10021 ttaatgtcga cccacatatt ccccccaacg gggttatttg ccgacatcag tgtcacctcc
    10081 agcttcgcag tcgatatccc atccgccacc agagaagtca catcagatga tgcggtgagt
    10141 actgcatttt tatcatccgc gacgaattta gattcggcag ttttcgtcga ttcctgaccg
    10201 tcggcctgac tcactttcgc cgtgagtgta tatttcgctg cgacagtggt cgtggcggtg
    10261 aaaatagcct gtccttgctc attagttttc gccgtcccat tgggagtcag aactaaattt
    10321 gccgggctgg cagtcagcgt cacttcgcta tctttcaaca ggttgttatt ggcatcagtc
    10381 accgtaactt tatacgtctg tttggcattg ccgtccgcca gtgcatagtt gccaatcacc
    10441 tcaataccgg agacggttgc ggtagcctta tcggctacaa acgttatagt ctgcgtattc
    10501 gaaggctggc tattccctgc cgatgccgtg atctcatacg ttccggcagt cactgaaacc
    10561 acctgcaact ctgctttccc ctcatcgttg gctttcaccc agacattatc gcctgtaagc
    10621 ggcttgacac cccggggcag attaaaggtc accaggctcc ctggaacagg gttaccattt
    10681 ttatcctcaa cgttcgccgt cagcgtaaag gcatctttgc cgtttgccac tttttgagca
    10741 gcggcgtcta cctgcaggtt tgcctgaccg gtgctggcat ccgcgttgaa tttcaccgtg
    10801 accgtcttct gagcaccatt caccgaagcg gaaatattgt gttctcccgc aaccgttgac
    10861 ataagctcaa tgtccgcttt acctgccgca ttagttgtta ctgtattccc cgtcttgctg
    10921 gtcacaccct gcggtaaagt cagcgtaact tcctgcccct gcaacgggtt accataggtg
    10981 tccacaacgg tcagggttat ctggttagca gactgtccat tagccagttg gttattaacc
    11041 ttcactgtca tatcacgaat ctcagcctta gatgcgtcac ctgcaacgtt cagcaccagt
    11101 ggctgagcaa cggcattttg gccgttcact cgcggcatca cagacaactg acccgcggca
    11161 gatcccagcg ttaaggtcga cacgtagacc ccattacctt tctctgtcca gtttcctgct
    11221 gaaggacgct cactccccgt gctggcggca ccgctaaaca ctggtgcatc tggcttcagc
    11281 ccggtgaccg ggttcccgta cgcatccttc acggtgaagg tgagttccgt cgtcgttttg
    11341 acggtcggag ccttattgtc cgcaacaagc gtagagttcg ctgatgacat ctctccggcg
    11401 atgaccgtca actgcgcggc ttcggtggct gctggctggc cgttgaagag aggcatgacg
    11461 cgaagctcgc ccgtctttcc acctgtagtc aacgtagcaa cataggaacc gttacctttt
    11521 tcggtccaac tggaaacggt cgccccttca gaggcggtcc ccgtcaaact tgccgacaac
    11581 gcaagaccac tgatagcgtt gccatgcgca tctttcgcca ccagcgtcac ggttgtgctt
    11641 tcgccggctt ttacgtgatc ttctgcgaca gagactttcg actggtttga agataacgca
    11701 tcagccacca cggttacttt tgccgcattt gctgccgcat tctgtccatt tagcttcggc
    11761 ataacttcta attcacccgc cgtagagccg agagtaatct gcgcagtcca cgtcccatca
    11821 ccattatttg tccagcccga tgccgtagaa ccttcagcag cggcacccgc taatgacggc
    11881 gcttccggcg tgaggctggt cacagggttg tcataggcat cttttaccgt ccagattgcc
    11941 gtaactgtcc ccccaaccac cggtttatca ggattcaggg tgatggacga atgtgctgca
    12001 tcaagcggcc cggcaacaaa cttcagcgtt tgttgtaatg tgcccagctg atactgctcg
    12061 ctgaaagcac gcacaacaac gtttccggca cgggtcgaag aaaccgcggc gctgtaaacg
    12121 cctggtttta tttccgaaat ggcaccaacg gttacaccat tagtgtcttg cggaacaaat
    12181 cgcaagcggc tggcttctcc cgtcaccgga ttaccctcgg agtccaccgc agtcaacgtc
    12241 aacgtatagg cttgctgacc atcagcaacc acgtcacctg acggctcgtt agcgctcagg
    12301 gtggagtttg ccacatccat catcgtggcc cgcagttctg cagtgacggt tttgctcatg
    12361 ccatcaacgc taacagtaat cgttgcctca cctgactgcg ttccggtagt aaagacagac
    12421 tgatacaccc ctgcttcagt ttcggtgaac tcacccagtg ttggctttgc ctgtgattta
    12481 gtggccttca gggaacgagt cacaatattt ccagccggtt tgaaagttag ttcagtcttg
    12541 atctgatctt tcatgcccgt gactggctgg ccctcggcgt cgcgcagaga cagcaccagc
    12601 ggtttttgct cattaccgtt agcaagcatt tgaatacggc tctgaccgtc aagcgttaac
    12661 gccgtgcgat cggcgctcat accagctccg gtaatgacca cctctgtctg cacgcgtttt
    12721 gaggtattgc ctttgttatc gtaggcaact gctgaaatcg cataataatt gtctttgcct
    12781 ggacgataag ccgggagcgt tacttgccac tgactaccct gaccggtaat tttgccacct
    12841 tcagccagta atgacggcgc ttcccactgc acatttttca gtccgtgagt tgctttgctg
    12901 accacaagcc ccagggaaag tgtctgaccg cccttacctt caatacgctc aggcagagca
    12961 atacggatca cttcagattt gcggtactca agaacgatgt tgttattacg ctcaaccagg
    13021 tcatagcggc tgcctgccag tacccgacgc tcgcgaatgc tatccgtatc gagttgtttc
    13081 gccaaaggtt cgccaattcg gtagttaact tccaggccaa agcgagtgtc attctcaccg
    13141 ctcttgccct gcttatgccc ggcgctcagt gtcagaagag gcactggcgt ataggtcacc
    13201 tcggcagaaa tagcatgcgg gtctttctgg cgcttatctt taccaaacag cccgacttca
    13261 tcgccataat actgttcata catcaggctt gcgccaagct gcggccaggc aggtaaatag
    13321 ccctctgcgc ggatatccca accattcgcc gggcgttcct gataatcctc aatatccggc
    13381 gattttttcc agccagaagc ccgaatataa ccattggcgc tcagtttcag ataatcgcgc
    13441 cagtattccg caccaacacc aatgcgggta tgactacggg ataaatcatg gtcgataaag
    13501 gtgttcaccc ccgccatcca gtcatttcct gaaaaatgac gccagccaaa accaatattt
    13561 gactgagtac gatcgtctgt acgatgtatt gccccctgag tgaacaacat atttgtcggc
    13621 gtatcataaa tcggataaag catttccagc gaagaatcct tcagcgagaa atctttatcg
    13681 acattcagtt tgacgcgcgc tgtaccatat ttcccgagcc actcctgtat ttcctggtta
    13741 gctttagcgg tggccattcc ggtaataaaa ttacgtgtcg catcgctatc tggctgactg
    13801 cttaaaaatg tcccggcatt tgcggcaaac gacgcgacat ttttctccac gttattatca
    13861 gcagttaccg tagtatttcc catgctcaac cgtggctgaa ccgcatgctg cgcacgtgcc
    13921 gccattactg gggtaaaggt gacagcgagt ggaaaaagaa cctgaacaga gatatttgcc
    13981 cacgccacgc agcgggccag aactgaataa cgaaatcgtg gttgtttatg acctgtttta
    14041 taacgtgaca ttgagataat ttcctgatga acgaattaac gaacataggc agacacaaat
    14101 aatggcgtct gcttgtcaga aaaaccgggg atttcccggt ttccttttcc gcagaaaagg
    14161 aaataccttt atgtaagaaa gtacaccgcc acctaatata tccagtgcct gtagccactc
    14221 atcaggtggt tgggcgcggg attatatttt tatttatggg tgaaaattta gctttttgtg
    14281 caagtcatta aatagatact taagttatat ttcttactct gcggacagtg aataattcat
    14341 tattcagagt gaataataat gacctgcctt taattagcaa ctattggaaa tacgtcgaag
    14401 taggtatata cgccaggata aaccgatata ttttttatgg atattcattt tccttcatcg
    14461 gactgtaaat tgcggagata tcgaacttgc actacccatc gcaacactca aacagattta
    14521 tgaatttcgt tcataacccc tgacaaatcc tgccgtctaa tcgagctctt ctctttacgt
    14581 tatgcttctc atgaacgata acggaaggat aacaccatgc aaaaagtaaa actgaataac
    14641 ggtattgaaa tgcccctgct gggctttggt gtatttcaga tgaccgatgc cgccgaatgc
    14701 gaaagagcgg tcattgacgc tattaacacc ggttatcgac tgattgacac cgccgcctcc
    14761 tatcaaaatg aaccccaggt cgggaacgca ctgaaacaaa gcggtatcgc ccgtaacgaa
    14821 ctctttgtca ccacgaaact ctggttgcag gataccagtt acgaaggggc caaagcccag
    14881 ttcgaacgct ctctgaatcg actgcaactt gattacgttg acctgtattt gattcatcag
    14941 ccttacggtg atgtccacgg tgcctggcgt gccatggaag agctgcaaca ggccggtaaa
    15001 attcgcgcta ttggcgtcag taacttccat cctgaccgac tggctgacct tatcgccttt
    15061 aataacgtcg ttccagcggt aaaccaggtt gaagttaacc ctttcaatca gcagctacac
    15121 gccgttccgt ggatgcaaag ccgtggtatt cagccagaag cctgggcacc gtttgcagtt
    15181 acagggcggt aagcgattat gcatctacct atttacgtcg gtcactcagt cagagtcttg
    15241 cccatattca atgaggttca acaccgcagc caatgaaaag taggccatgc ggggattaat
    15301 agcattactc attaaccaat cgctcttttc atttacgtgc tttttatgta acatattgct
    15361 tttattgatc ttcctggagt ttgcatgaat aagaatctca tcctcgcatt tgcattattt
    15421 tccttacctg tatttgcaga agaagatctg gggccaggca aatatgtttg tgacattagg
    15481 atttccagcc tggataccgc tactcaaatt ctgtcaaaat ccgcaacagt tctcgataat
    15541 ggaaacaatt tcatcgttca aatgccaaat ggagatcaat tgtactctcc agatctggaa
    15601 aatgttgatg atggtataaa acaaaaagca acaataggtg gagtgacatt tattcgtcgt
    15661 ccaactttta acgatcgctt catcgtggaa gatggaaata caggattctt ctataagatg
    15721 cgaaactgcg agaagaaata aaggaagttt gatagcgata tcctcctgat ttcaggcttt
    15781 gtacctgttc aagacttaca actacaagcc gctacgtgtt aacgcctccc aaaataacca
    15841 ccgttaaccg tagcggtcat agatactatt attttaacca cattactggt gataatgata
    15901 attgatatta tggccgtata gaaaataagt attaattatc agaaacttta ttatgtaaac
    15961 gcatcctaat gattacgtta ttcctcttac cgatattcgc ccagagcaac catcaattac
    16021 acatatcatc aattttcgag ttttactctt ttctccacca taaaatcaat aaaagcacga
    16081 actttagagg gcattctcac ccgtgatgaa acataaatat aaaacccagg aaatgaaaca
    16141 gcccaatcct caagaatcgg ctgtaacttg cccgcattga tatgttcagc gattgcaaaa
    16201 tccagatgtt gaatgatacc cgttccattc agcgccattc gtatcatgct ttcgtcatca
    16261 ttagaaatat acttacctga aacctctatt tcgaactctt cattaccccc cacaacctta
    16321 gaaaactccc atgcagagag tgcaccgcta cttttatgcc gcaaccgaag gcaattatgg
    16381 ttactcagtt cctgaggcgt ttcaggaata ccgtgttctt ttaaataatc tggcgatgcc
    16441 acagtaacca gtttaataag cggagaaaca ggaacggcag tcatatgctc atccagtcct
    16501 tgctcaagac gtatgcccac atcacagccg tccgcaataa tgttagataa accgtcatcc
    16561 ataatgagtt ctatctttgt tttggggtaa cgagtaagaa actcgccaat atgaggctca
    16621 acaagcatcc gtgctgcaac acgggaggaa tttattctca acaggcctgt aggttcaagt
    16681 tgggtatcac caacatcttt caaagcatca tcaatttgcc ccaaagccga caccagcact
    16741 tcatacagac gctgtccttc ctcagtaagc gagatattgc gggtcgtacg gtatatcagc
    16801 ttggtgttaa gacgttcctc aagagatttt aaattctggg acaatgcggc cagcgtaata
    16861 cccatttcag aagcggctct ggaaaaactg ccggcacgaa caatcagcgc aaaccaggca
    16921 agagaaggca aaattgaagg ctctattttc atcattgtaa accttaactt aacactgtga
    16981 tagatattag accattgatg ataaccaata caagtcatac tattacgccg ttctcgatat
    17041 gaacacaaat gccagttgcg gataatagaa atgatattta aatctgttta ggcaaatccc
    17101 cggagcttga aatgaaaact gtttcgataa aacacactta ctgggatata gctgctgata
    17161 tctatttccc tgacgatttt aatagtgaaa aaaagtatcc tgccattatc agcgcccatc
    17221 caattggaag ctgcaaagaa cagacatcgg gtagtgtgta tggtgccgcc ctggcaaaag
    17281 ctggctttat cgtcattgct tttgatgcta gttttcaggg aagcagcggt ggggaaccgc
    17341 gttatctgga agatccaaca atgcgagtaa aagacttcag cattgtcgtt gattacctta
    17401 ccagcttacc ttacgttgat gccgggcgta ttggtgtact gggtatttgt ggtggaggtg
    17461 gatatgccat taatgccgca atgacagaac gccgaattaa agctattggt actgtcacgg
    17521 gtgcaaacta cgggcgtctt atgcgtgagg gatttacagc gtttaatcct atcggagcac
    17581 ttgaagcaat ggcgcaacaa agaacagacg aagctaacgg cgcaaaactt cgcgtagatg
    17641 atttactccc ctcttctccg gatgcagctc ttgaagcggg actaacagaa atagaccttt
    17701 ttgaagccac agaatattat cgttcgccac gaggctatgc acctaatggg gtaaatcgtt
    17761 cgctattctc acatcagact gttgccgtgg gttgggatgc atttcatctt gcagaagtgc
    17821 tgttaactca accaataatg gttgtggtgg gcgatagagt gggcgcattt ggtgcctacc
    17881 gtgacggctg cgaaattatt ggccgcgctg cttcaaaaca taaagagctg gtcgtggttg
    17941 aaggttattc acattacgac ctgtatgata agccagaacc tgtgaaacag gcactcgata
    18001 aattaattcc tttttataaa acacatttgt aataatttta cagggagatc ccttatctcc
    18061 ctgaca
//
LOCUS       DAZFBR010000096        17968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFBR010000096 DAZFBR010000000
VERSION     DAZFBR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.096"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            109..777
                     /locus_tag="ACHJZ2_003588"
     CDS             109..777
                     /locus_tag="ACHJZ2_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO4569629.1"
                     /translation="MFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNKIDLLE
                     KQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKYFILNN
                     GSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEFARWQQ
                     LLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQK
                     RSKK"
     gene            821..5587
                     /gene="ehaG"
                     /locus_tag="ACHJZ2_003589"
     CDS             821..5587
                     /gene="ehaG"
                     /locus_tag="ACHJZ2_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO4569630.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            5955..7610
                     /gene="lldP"
                     /locus_tag="ACHJZ2_003590"
     CDS             5955..7610
                     /gene="lldP"
                     /locus_tag="ACHJZ2_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO4569631.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7610..8386
                     /gene="lldR"
                     /locus_tag="ACHJZ2_003591"
     CDS             7610..8386
                     /gene="lldR"
                     /locus_tag="ACHJZ2_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO4569632.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8383..9573
                     /gene="lldD"
                     /locus_tag="ACHJZ2_003592"
     CDS             8383..9573
                     /gene="lldD"
                     /locus_tag="ACHJZ2_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO4569633.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9759..10232
                     /gene="trmL"
                     /locus_tag="ACHJZ2_003593"
     CDS             9759..10232
                     /gene="trmL"
                     /locus_tag="ACHJZ2_003593"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO4569634.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10285..11106)
                     /gene="cysE"
                     /locus_tag="ACHJZ2_003594"
     CDS             complement(10285..11106)
                     /gene="cysE"
                     /locus_tag="ACHJZ2_003594"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO4569635.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11186..12205)
                     /gene="gpsA"
                     /locus_tag="ACHJZ2_003595"
     CDS             complement(11186..12205)
                     /gene="gpsA"
                     /locus_tag="ACHJZ2_003595"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO4569636.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12205..12672)
                     /gene="secB"
                     /locus_tag="ACHJZ2_003596"
     CDS             complement(12205..12672)
                     /gene="secB"
                     /locus_tag="ACHJZ2_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO4569637.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12735..12986)
                     /gene="grxC"
                     /locus_tag="ACHJZ2_003597"
     CDS             complement(12735..12986)
                     /gene="grxC"
                     /locus_tag="ACHJZ2_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO4569638.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13128..13559)
                     /gene="yibN"
                     /locus_tag="ACHJZ2_003598"
     CDS             complement(13128..13559)
                     /gene="yibN"
                     /locus_tag="ACHJZ2_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO4569639.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13804..15348
                     /gene="gpmM"
                     /locus_tag="ACHJZ2_003599"
                     /gene_synonym="pgmI"
     CDS             13804..15348
                     /gene="gpmM"
                     /locus_tag="ACHJZ2_003599"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO4569640.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15382..16641
                     /gene="envC"
                     /locus_tag="ACHJZ2_003600"
     CDS             15382..16641
                     /gene="envC"
                     /locus_tag="ACHJZ2_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO4569641.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16645..17604
                     /gene="yibQ"
                     /locus_tag="ACHJZ2_003601"
     CDS             16645..17604
                     /gene="yibQ"
                     /locus_tag="ACHJZ2_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO4569642.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17591..17968)
                     /gene="waaH"
                     /locus_tag="ACHJZ2_003602"
                     /pseudo
     CDS             complement(17591..17968)
                     /gene="waaH"
                     /locus_tag="ACHJZ2_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
BASE COUNT         4499 a         4641 c         4702 g         4126 t
ORIGIN      
        1 ctgtcattaa tgtaaagcaa catagatcac caataataca atggacaact ctaccggcat
       61 tgatcgctgg gcggaggaaa taaggatttt tacatgaact agaagaagat ttttttcagt
      121 gctgtcactg tttcagttct ctgcgcatta acgggatgtg actacattga agaaggcaag
      181 ccagaaagta gtttattgaa acaacaagaa gaacataaca ataaaattga tctacttgaa
      241 aaacaacaag cacagctaaa gagtcaactg gaaactattc aaaaacaaca aactggcata
      301 ataagcagta caaagacttt aactcacgta attaaatccg ttaaagatca acaaaacact
      361 tttattttta ccgagtttaa cccagcaaaa acgaagtatt tcatcctgaa caatggttcg
      421 gtggctttag cgggtcgagt gttatccatt gacgccacag agaatggcag tgttattcat
      481 atttcactgg tcaacttatt aagtacacct atctcaaata ttggttttaa tgcgacatgg
      541 ggtggtgaaa aacctgtcga tgccaaagag tttgcccggt ggcaacaatt acttttcaac
      601 acatcaatga aatccacatt gaaattatta ccaggtcaat ggcaagacat taatttgacc
      661 ctgaagggtg tatcgcccaa taacctggga tatctgaagt tagccatcaa catggaaaat
      721 attcagttcg acaatcttcc ctctgctgaa aatcggcaga aaagaagcaa aaaataaatg
      781 cagttagtac gcttataaca aggaatagcg agcatcatta atgaacaaaa tatttaaagt
      841 tatctggaac cctgcgacag ggaattatac tgttaccagc gaaacggcaa aaagccgtgg
      901 caagaaatct gggcgcagta agctgttaat ttctgcgctg gttgcgggtg gaatgttgtc
      961 gtcgtttggg gcattggcga atgccgggaa tgacaacggt cagggtgttg attacggtag
     1021 tggatcagct ggcgacggct gggttgctat aggcaaaggg gcgaaagcaa atacttttat
     1081 gaacaccagt ggttccagta ctgctgtggg ttatgacgct atagctgaag gccaatatag
     1141 ctctgccatc gggtcaaaaa cccatgcgat tggtggtgca tcaatggcct ttggggttag
     1201 tgcaatatca gaaggcgata gaagtatagc actgggtgcc tcttcgtatt cattgggcca
     1261 atactcaatg gccctcggcc gttattcaaa agcattgggt aaattgtcta ttgctatggg
     1321 ggactcttcc aaagcggaag gagcaaacgc cattgccctg ggaaatgcca ctaaagctac
     1381 tgagattatg agtattgctc ttggcgacac cgccaatgcg tcaaaagcgt attcaatggc
     1441 gctgggagca agtagcgtcg catctgaaga aaacgctatt gcgataggtg ctgagaccga
     1501 agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga ctaatagcat
     1561 ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag gaaatggcag
     1621 ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg atggcgtgga
     1681 tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg ccttaggcac
     1741 agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca gtgccaacgg
     1801 tattaactct gtcgcgctgg gcgcagattc cattgcggat ttagacaata ccgtctctgt
     1861 cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga tcaagtctga
     1921 cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg tagcaaaaag
     1981 gcttggagga ggggctgcag tagatgttga tgacggtact gttacagcac caacctacaa
     2041 tttaaaaaat ggtagcaaaa ataacgtagg ggctgcgctc gctgtacttg atgaaaacac
     2101 cctgcaatgg gaccaaacca aaggcaaata cagcgctgct catggtacta gtagcccaac
     2161 tgccagcgta atcaccgatg ttgcggatgg cacgatttca gcctccagta aggatgcggt
     2221 taacggttcc caactgaaag ctaccaatga cgatgtcgaa gccaacaccg ccaatatcgc
     2281 tactaatacc agcaacattg ccacgaatac ggcaaatatt gccaccaata ccaccaatat
     2341 caccaacctg acggattccg ttggtgacct tcaggctgat gccctgctct ggaacgaaac
     2401 taaaaaggca ttcagtgcag ctcacggcca ggataccacc agcaaaatca ccaacgttaa
     2461 agatgccgac ctgacggctg acagcactga tgctgttaac ggctctcagc tgaaaaccac
     2521 caacgatgct gtggcgacga ataccaccaa tatcgccaat aacacttcca atattgccac
     2581 taacaccacc aacatctcta acctgactga gacggtgact aatcttggtg aggatgcgct
     2641 gaaatgggat aaggacaatg gtgtattcac ggcagctcat ggcaccgaga ccaccagcaa
     2701 aatcaccaac gttaaagatg gcgacctgac gactggcagc accgatgccg ttaacggctc
     2761 tcagctgaaa accaccaacg atgccgtggc gacgaatacc accaatatcg ccactaacac
     2821 caccaacatc tctaatctga ctgagacggt gactaatctt ggtgaggatg cgctgaaatg
     2881 ggataaggac aatggtgtct tcactgcagc tcatggcaac aataccgcca gcaaaatcac
     2941 caatatcctg gacggcacag tcactgcaac cagttccgat gccattaacg gtagccagct
     3001 ttatgactta agcagcaata tcgccaccta cttcggcggc aatgcttctg tgaatactga
     3061 cggtgtgttt accggtccaa cctacaaaat cggtgaaaca aattattata acgtcggcga
     3121 tgcactggct gcgattaact cctcatttag cacgtctctc ggcgatgctc tgctttggga
     3181 tgccaccgca ggtaaattca gtgccaaaca cggtactaat ggtgacgcaa gcgtgatcac
     3241 tgatgtcgca gatggtgaaa tttcagactc cagttctgac gcagtaaacg gctcacaact
     3301 ccacggcgtg agcagttatg ttgttgatgc gctggggggt ggtgccgaag tcaatgcaga
     3361 cggcaccatc actgcgccga cgtacaccat tgctaatgct gattacgata atgtcggtga
     3421 tgccctgaat gctatcgata ccactcttga cgacgctctg ctctgggatg cggacgccgg
     3481 tgaaaatggt gcatttagcg ccgctcacgg aaaagataaa actgccagtg taatcactaa
     3541 cgtcgctaac ggtgcaatct ctgctgccag cagcgacgcg attaacggct cacaactcta
     3601 taccaccaat aagtacatcg ctgatgcgct gggtggtgac gcagaagtca acgctgacgg
     3661 caccatcacc gcaccgactt acaccattgc gaacgccgag tacaacaacg tcggtgacgc
     3721 cctggatgcg cttgatgata acgccctgct gtgggatgag actgccaatg gcggtgctgg
     3781 agcctacaat gccagccatg acggtaaagc cagcatcatc actaatgtcg ctaatggcag
     3841 tattagtgag gacagtaccg atgcagtgaa cggttctcag ttgaatgcga cgaatatgat
     3901 gattgagcag aacacccaaa ttatcaatca gctcgctggt aacaccgacg caacctatat
     3961 ccaagaaaac ggtgcgggta ttaactatgt gcgtactaac gacgacggct tagcgttcaa
     4021 cgacgccagc gcacagggtg ttggcgctac agctataggt tataactctg tcgccaaagg
     4081 cgatagcagc gtagctattg gtcagggcag ctacagcgac gttgatacgg gtatcgccct
     4141 gggtagcagc tctgtttcca gccgagtgat tgccaaaggc tcccgtgaca ccagcataac
     4201 ggaaaatggc gttgttattg gttacgacac cacggatggc gaactgctcg gtgcattgtc
     4261 tatcggtgat gacggtaaat atcgtcaaat catcaacgta gccgatggtt ccgaagccca
     4321 tgacgccgtt acggttcgtc aattgcagaa tgcgattggt gcggtcgcaa ccacgccgac
     4381 taaatacttc cacgctaatt caacggaaga agattcactg gcagtgggaa ctgactcgct
     4441 ggcaatgggt gcgaaaacca tcgtgaatgg cgataaaggt attggtatcg gttatggtgc
     4501 ctacgtggac gcgaatgcac ttaacggcat tgccattggt agcaatgcgc aagtcattca
     4561 tgtcaacagt attgcgatag gtaatggttc tacgaccact cgtggcgctc aaaccaatta
     4621 taccgcctac aacatggacg caccgcagaa ctctgtcggt gaattctcag tcggtagtgc
     4681 ggatggtcaa cgtcagatca ctaacgtcgc agcaggttcg gctgataccg atgcggtcaa
     4741 cgtgggtcag ttgaaagtaa cggatgcgca ggtttcccag aatacccaga gcattactaa
     4801 cctggataat cgggtaacga atcttgattc acgcgtcacc aatatcgaaa acggtattgg
     4861 cgatatcgtc accaccggta gcaccaagta cttcaagacc aataccgatg gtgtagatgc
     4921 cagcgcgcag ggtaaagata gcgtcgcgat tggttccggc tccattgctg ccgctgacaa
     4981 cagcgtcgct ctgggtacag ggtctgtggc aaccgaagaa aatacgatct ctgtaggttc
     5041 ctctactaac caacgtcgta tcaccaacgt agctgcaggt aaaaatgcta ccgatgctgt
     5101 taacgtggca cagttgaagt cttccgaagc tggcggtgta cgttacgaca ccaaagctga
     5161 tggttctatc gactatagca atatcaccct cggtggcggc aacggcggta cgactcgtat
     5221 cagcaacgtc tccgctggcg tcaacaacaa cgacgtggtg aattacgcgc agttgaagca
     5281 aagcgtgcag gaaacgaagc aatacaccga tcagcgaatg gttgagatgg ataacaaact
     5341 gtctaaaact gaaagcaagt tgagcggtgg tatcgcttct gcaatggcaa tgaccggtct
     5401 gccgcaggct tacactccag gtgccagcat ggcctctatt ggtggcggta cttacaacgg
     5461 tgaatcggca gttgctttag gtgtatcgat ggtgagcgcc aatggtcgtt gggtctacaa
     5521 attacaaggt agtaccaata gccagggtga atactccgcc gcactcggtg ccggtattca
     5581 gtggtaatga tccattaagt tagtgtgact aagggcaaac gccaaattgc ctgatgcgtt
     5641 acgcgcatca ggcaagataa ggcgctccgg gtcaacaatc taagggcaat tctctgatga
     5701 ggattgccct tttctttacc agacatctcc ccccacaaga attggcccta ccaattcttc
     5761 gcttatctga cctctggttc acaattcccc aattaaaact cacatcaata ttgccaatac
     5821 ataacattta gttaaccatt cattgtcatt atccctacac aacacaattg gcagtgccac
     5881 ttttacacaa cgtgtgacaa ggagatgagc aacagactca ttacacgatg tgcgtggact
     5941 ccaggagacc tgcaatgaat ctctggcaac aaaactacga tcccgccggg aatatctggc
     6001 tttccagttt gatagcatcg cttcccatcc tgtttttctt ctttgcgctg attaagctca
     6061 aactgaaagg atacgtcgcc gcctcatgga cggtagcaat cgcccttgcc gtggctttgc
     6121 tgttctataa aatgccggtc gctaacgcgc tggcctcggt ggtttatggc ttcttctatg
     6181 gtttgtggcc catcgcgtgg atcattattg cagcagtgtt cgtctataag atctcggtga
     6241 aaaccgggca gtttgacatc attcgctcgt ctattctttc gataacccct gaccagcgtc
     6301 tgcaaatgct gatcgtcggt ttctgtttcg gcgctttcct tgaaggagcc gcaggctttg
     6361 gcgcaccggt agcaattacc gccgcattgc tggtcggcct gggttttaaa ccgctgtacg
     6421 ccgccggact gtgcctgatt gttaacaccg cgccagtggc atttggcgcg atgggcattc
     6481 caatcctggt tgccggacaa gtaacaggca tcgacagctt tgagattggc cagatggtgg
     6541 ggcggcagct accgtttatg accattatcg tgctgttctg gatcatggcg attatggacg
     6601 gctggcgcgg tatcaaggag acgtggcctg cggtcgtggt tgcgggcggc tcgtttgcca
     6661 tcgctcagta ccttagctct aacttcattg ggccggagct gccggacatt atctcttcgc
     6721 tggtatcact gctctgcctg acgctgttcc tcaaacgctg gcagccagtg cgcgtattcc
     6781 gttttggtga tttgggggcg tcacaggttg atatgacgct agcccacacc ggttacactg
     6841 cgggtcaggt gttacgtgcc tggacaccgt tcctgttcct gacagctacc gtaacactgt
     6901 ggagtatccc gccgtttaaa gccctgttcg catcgggtgg cgcgctgtat gagtgggtga
     6961 tcaacattcc ggtgccgtac ctcgataaac tggttgcccg tatgccgcca gtggtcagcg
     7021 aggctacagc ctatgccgcc gtgtttaagt ttgactggtt ctctgccacc ggcaccgcca
     7081 ttctgtttgc tgcactgctc tcgattgtct ggctgaagat gaaaccgtct gacgctatca
     7141 gcaccttcgg cagcacgctg aaagaactgg ctctacccat ctactccatc ggcatggtgc
     7201 tggcgttcgc ctttatctcg aactattccg gactgtcatc aacactggca ctggcactgg
     7261 cgcacaccgg tcatgcattc accttcttct cgccgttcct cggctggctg ggggtattcc
     7321 tgaccgggtc ggatacctca tctaacgccc tgttcgccgc gctgcaagcc accgcagcac
     7381 aacaaattgg cgtctctgat ctgttgctgg ttgccgccaa taccaccggt ggcgtcaccg
     7441 gtaagatgat ctccccgcaa tctatcgcta tcgcctgtgc ggcggtaggc ctggtgggca
     7501 aagagtctga tttgttccgc tttactgtca aacacagcct gatcttcacc tgtatggtgg
     7561 gcgtgatcac cacgcttcag gcttatgtct taacgtggat gattccttaa tgattgtttt
     7621 acccagacgc ctgtcagacg aggttgccga tcgtgtgcgg gcgctgattg atgaaaaaaa
     7681 cctggaagcg ggcatgaagt tgcccgctga gcgccaactg gcgatgcaac tcggcgtatc
     7741 acgtaattca ctacgcgagg cgctggcaaa actggtgagc gaaggcgtgc tgctcagtcg
     7801 acgcggcggc gggacgttta ttcgctggcg tcatgacaca tggtcggagc aaaacatcgt
     7861 ccagccgcta aaaacgctga tggccgatga tccggattac agtttcgata ttctggaagc
     7921 ccgctacgcc attgaagcca gcaccgcatg gcatgcggca atgcgcgcca cacctggcga
     7981 caaagaaaag attcagcttt gctttgaagc aacgctaagt gaagacccgg atatcgcctc
     8041 acaagcggac gttcgttttc atctggcgat tgccgaagcc tcacataaca tcgtgctgct
     8101 gcaaaccata cgcggtttct tcgatgtcct gcaatcctca gtgaagcata gccgtcagcg
     8161 gatgtatctg gtgccaccgg ttttttcaca actgaccgaa caacatcagg ctgtcattga
     8221 cgccattttt gctggtgatg ccgacggggc gcgtaaagca atgatggcgc accttagttt
     8281 tgttcacacc accatgaaac gattcgatga agatcaggct cgccacgcac ggattacccg
     8341 cctgcccggt gagcataatg agcattcgag ggagaaaaac gcatgattat ttccgcagcc
     8401 agcgattatc gcgccgcagc gcaacgcatt ctgccgccgt tcctgttcca ctatatggat
     8461 ggtggtgcat attctgaata cacgctgcgc cgcaacgtgg aagatttgtc agaagtggca
     8521 ctgcgccagc gtattctgaa aaacatgtct gacttaagcc tggaaacgac gctgtttaat
     8581 gagaaattgt cgatgccggt agcactggca ccggtgggtt tgtgtggcat gtatgcgcgt
     8641 cgtggcgaag ttcaggcagc caaagcggcg gacgcgcatg gcattccgtt tactctctca
     8701 acggtttccg tttgcccgat tgaagaagtc gcgccagcca tcaagcgccc aatgtggttc
     8761 cagctttatg tactgcgcga tcgcggcttt atgcgtaacg cgctggagcg agcaaaagca
     8821 gcgggttgtt cgacgctggt tttcaccgtg gatatgccga caccgggagc gcgttatcgt
     8881 gatgcgcatt ctgggatgag cggcccaaat gcggcaatgc gccgctactt gcaagcggtg
     8941 acgcatccgc aatgggcgtg ggatgtgggc ctgaacggtc gtccgcatga tttaggtaat
     9001 atttcggctt acctcggcaa accgaccgga ctggaagatt acatcggctg gctggggaat
     9061 aacttcgatc cgtccatctc atggaaagac cttgagtgga tccgcgattt ctgggatggc
     9121 ccgatggtga tcaaagggat cctcgatccg gaagatgcgc gcgatgcagt acgttttggt
     9181 gctgatggga ttgtagtttc taaccacggt ggccgccagc tggacggtgt actctcttcc
     9241 gctcgtgcgt tgcccgctat tgcagatgcg gtgaaaggtg atatcgccat tctggcggat
     9301 agcggaattc gtaacgggct tgatgtcgtg cgtatgattg cgctcggtgc cgacaccgta
     9361 ctgctgggtc gtgctttcct gtatgcgctg gcaacagcgg gccaggcggg tgtagctaac
     9421 ctgctaaatc tgatcgagaa agagatgaaa gtggcaatga cgctgactgg cgcgaaatcg
     9481 atcagtgaaa ttacgcaaga ttcgctggtg caggggctgg gtaaagagtt gcctacggca
     9541 ctggctccga tggcgaaagg gaatgcggca tagtcgtttg agccccctca ccctaaccct
     9601 ctccctcagg gagtggggac cgttcggcgc tgtatgtact ccctcactct gaaacgacac
     9661 cgcactcttt ttttctccct cgcccctccg gggtgagggg aaaaggccgc actgctcacc
     9721 aattttctgc tatcctgccc ccgcactaag ggggcagtat gctaaacatc gtactttacg
     9781 aaccagaaat tccgccaaat actggcaata tcatccgtct ttgcgctaat accggctttc
     9841 gtctgcatat catcgaaccg atgggatttg cctgggacga taagcgcctg cgccgcgcgg
     9901 ggctggacta tcacgagttt accgccgtta cgcgtcatca tgactttcgc gcgttcctcg
     9961 aagcagaaaa tccccagcgc ctgtttgccc tcaccacgaa aggtacgcct gctcacagcg
    10021 ccgtaaacta tcaggatggc gattatctga tgttcggccc ggaaacacgc ggcctgccag
    10081 cgagcattct tgatgccctg cccgctgaac aaaaaattcg cattccgatg gtgccggaca
    10141 gccgcagcat gaacctgtcc aatgcggtgt cagttgtggt gtatgaagcc tggcggcagc
    10201 tgggatatcc gggagcgttg ttgagagatt agttactgta tgccggatag atgattacat
    10261 cgcatccggc acgatcacag gacattagat cccatcccca tactcaaatg tatggttaat
    10321 accgttgaaa tgctggtcca tatccattga tggcttatcg ctgtctggtt taccgacaat
    10381 acgagccgga acgccagcgg cggttgtatg cggcggcacc ggttgcagca ccacggaacc
    10441 tgcgccaatc ttcgcgccgc gaccaacttc aatattgccg aggattttcg cgcccgcgcc
    10501 aatcatcaca ccttcacgaa ttttcgggtg acggtcacca ccagatttac ccgtaccgcc
    10561 aagcgtcaca gattgcagaa tcgatacgtc gttttcaatt accgccgttt cacccacaac
    10621 gatgcccgtg gcgtggtcga gcataatgcc acggccaatt tttgctgccg ggtgaatatc
    10681 gacctggaac gtcacagaaa cctggttttg cagaaagatt gccaatgcgc gacgcccctg
    10741 attccacaac cagtgaccga tgcgataggc ctgcaaggca tgaaaaccct tcaggtataa
    10801 caacggggtt gagtatttat cgactgccgg gtcgcgggta cgcaccgcct gaatatcaca
    10861 ggccgcagag gcgatcattt ccgggtcagc ggcgtaggct tcttccacca cttcacggat
    10921 agcaatagca ggcataattg gcgatgacag cttgttcgcc agcatgtagc tcagtgcact
    10981 accaaggttt tcgtgcttga gtagcgtcgc gtggtaaaaa ctggccagca ttggctcaca
    11041 gtccgccagc gttctggctt cggctttaat attgttccag acaatttcca gttcttcaca
    11101 cgacattgct tactccacac gatgagataa tgaccggccc gttctgcgcg ggccgggtca
    11161 tagcggtaac aaaggttccc tggggttagt ggctgctgcg ctcgtccttg cgtgcacgac
    11221 ctaataacgt caatgctgcc tcgcgcgcgt tttttccgca atataatact tgataaattt
    11281 cctcggttat tggcatttct acgccgaagc gatgcgccag ttcgcggact tctttcgtat
    11341 tgcggtagcc ttccaccacc tgaccaatct tctcctgcgc gctttgtaca tccatgccct
    11401 gaccgagcat catgccaaaa cggcggttac gcgactggtt gtcggtacaa gtaagcacaa
    11461 gatcgcccag ccccgccatg cccataaagg tggcaggatc ggcacccagc gccgcgccaa
    11521 gacgcgacat ttcagccagc ccacgggtga tcagtgccgt acgcgcattc gcaccaaaac
    11581 cgataccgtc ggacatcccc gcgccaatgg caatgacgtt tttcaccgca ccgccaagct
    11641 gcacgccaat gaaatccgga ttgctgtaaa cgcggaaact tttgccgcag tgcagcagct
    11701 gctggagatc atcggcaaag gtctgatcgg tcgaggccag cgaaatagct gtcggtaaac
    11761 ctgccgccag ttctttcgca aacgttgggc cagagataac cgccagcgga atttgatcgc
    11821 ctaaggcctc acgcgccacg tcctgtaaca gacgtccggt ttccgcttcc agccctttgg
    11881 tcgcccacac cagacgcgca tcaggacgca tcagcggttt aatctggcgc agcacttcac
    11941 caaagacatg gctgggtacg acgacgagaa tattacggct ggctgccagc gcagtggcga
    12001 gatcgctttc aagatggagc gtatcgggaa aaggcacatc ggggagaaac gcggcgttac
    12061 agcggtcgcg ttcaagcgtt gcgatatgtt cagggtcatg gccccagagg acaacctcgt
    12121 ggccatttct tgccagggtg atggcaagag cggtgccgta cgagccggca ccgatcacag
    12181 tcattgaagc attacgttgg ttcatcaggc atcctgatgt tcttcagtac cttcgccagc
    12241 ctgctgctgc aaatagttca tgaacagcgc atcgaagtta accggcgcaa ggttcagttg
    12301 cgggaatgta ccgcgggata ccatgctggt gatgcactca cgagcatacg ggaacagaat
    12361 gttcgggcag tatgcgccca ggcaatgtgc catctgggtg ccttcgatac ccgcgatgga
    12421 gaaaataccg ccctgctgaa cttcacacag gaacgcggtt tcttcgccca gggaggccgt
    12481 tacggtaaca cgcagtacca cttcgtatac gtcatctgcc agttgggaag atgccgtatc
    12541 cagatcaagt ttaacttctg gttgccaatc tttctggaaa acgtgcggcg cgttcggcgc
    12601 ttcgaaagag atatccttgg tataaatacg ttggatctgg aaagtcattt cagtgttgtt
    12661 ttgttctgac atgtgtagaa aacccttaag tgttgtcctt aaatactgcg caatgtaatg
    12721 ccaacgttca cacgttattt cagcagggga tccagtccac cacgtgcatc caatgcatac
    12781 aagtcatcac agccgccaat gtgctgtgcg tcaataaaaa tctggggaac cgtggtgcga
    12841 ccgctgcgtt tgatcatctc ttcacgcttg gcggcattgc catcgatcgg caactcctga
    12901 aaactcacgc ccttgctgct cagcagtgct tttgcacgat ggcaatacgg gcaggtttct
    12961 ttggtataga tttcaacatt ggccataatt tagctcctgt ttttttaccc tgaaatttca
    13021 tgttgcaggg cggctgcaaa atggagtgtc cccagaagct tataacaata agtcgctggg
    13081 ggcctaattg cagctaacgg cctgcatcat gaaagacgac aggtaaatta tttgccgcgc
    13141 accaaaggca agttttcgcc agcccagcca gcgacgcctt ctttcagcac gaatacttgc
    13201 gcaaaaccag ctttcgtcag tgcgtttgca ggctcctggc actgcatgcc agaaccgtct
    13261 accacgataa ccggtttgtc tttgtgcttc tcaagctcac caacattgtt ggctttgatt
    13321 tcgctcggca acagattgat agaacctgcg atatggcctt tacggaagtc atcacgctga
    13381 cgtaaatcca caaccacagc gtcttctttg ttgatcagac gcgtagcttc accacgagta
    13441 atcaccttca ctttcgaggt caggctctta aacgtagtca caagaaccgc caccagtaac
    13501 gcgatccagg cgatactcag tatgggatga cggccaacaa attgcataat ttcttgcatg
    13561 gggggtaaca actcccgacg tagtgattaa aaaaaccagg aaaggagtat acctgcgcgg
    13621 tgcggcaaat acagccagcg cgttaactgg aatgcaattt tgcggggcac gacgaaaaaa
    13681 aagcgcaaat ctggcatcgg cttgccgcga gcggtcgtat attttgatct tcagaggcta
    13741 ttttatcgat tcagctgtag taaaattacg caaattttga ctcttgagta tgaggttgtc
    13801 gcaatgtcgg tttctaaaaa acctatggta ctggtgattc tggatggcta tggctatcgt
    13861 gaagaacagc aggataacgc catttttagt gctaaaaccc cggtaatgga tgcactgtgg
    13921 gccaatcgtc cgcataccct gatcgacgct tccggtctgg aagtcggtct gcctgaccgt
    13981 cagatgggta actccgaagt aggccacgtt aacctgggtg ccggccgcat cgtgtatcag
    14041 gacctgactc gtctggacgt tgaaatcaaa gatcgcgctt tctttgctaa tccagtgctg
    14101 actggcgcgg tagataaagc gaaaaacgca ggtaaagcag tacacattat gggtctgctc
    14161 tccgcaggcg gcgtacacag ccacgaagat cacatcatgg cgatggtaga actggcagct
    14221 gaacgcggcg cagaaaaaat ctacctgcac gcattccttg acggtcgcga caccccgccg
    14281 cgcagtgctg aatcctcgct gaaaaaattt gaagagaaat ttgctgcgct gggtaaaggc
    14341 cgcgtagcgt ccatcattgg tcgttactac gcgatggacc gcgataaccg ttgggatcgc
    14401 gttgaaaaag cctatgacct gctgactctg gcgcagggtg agttccaggc cgatactgcc
    14461 gttgctggtt tgcaggcagc ttatgctcgc gacgaaaacg acgagttcgt gaaagcgacc
    14521 gttattcgtg cagaaggtca gccagatgcg gcaatggaag acggcgatgc gctgattttc
    14581 atgaacttcc gtgctgaccg cgcgcgcgaa atcactcgtg cattcgtaaa cgcagatttc
    14641 gacggcttcg cgcgtaagaa agtggttaac gtcgatttcg tgatgctgac cgaatacgcc
    14701 gctgacatca aaactgcggt tgcttaccca cccgcttccc tggttaacac cttcggcgag
    14761 tggatggcga aaaacgacaa aactcagttg cgtatttccg aaaccgaaaa atatgcccac
    14821 gttactttct tcttcaacgg tggcgtagaa gagtcgttca aaggcgaaga tcgcattctg
    14881 atcaactcac cgaaagtggc tacctacgat ctgcaaccgg aaatgagctc cgcagagctg
    14941 accgaaaaac tggttgcggc catcaagagc ggcaaatacg acaccattat ctgtaactat
    15001 ccgaacggcg acatggtagg tcacaccggg gtgatggaag cggcggttaa agcggttgaa
    15061 gcactggatc actgcgtgga agaagtcgcg aaagcggttg agtccgtggg tggacaactg
    15121 ctgatcaccg ctgaccacgg taacgctgaa cagatgcgcg atccggcaac gggtcaggca
    15181 cacacggcac acaccaacct gccagttccg ctgatttacg ttggtgataa gaacgtgaaa
    15241 gcggttgcag gcggcaaact ttctgacatc gcgccgacca tgttgtcgct gatgggtatg
    15301 gaaatcccgc aagagatgac tggtaagccg ctgttcatcg tggaataatc cctccccatg
    15361 aggggaaagg cgattaatac catgacacgg gccgtgaaac cgcgcaggtt tgcaatcagg
    15421 cccatcatct acgccagcgt tcttagcgct ggcgtattgt tgtgcgcctt ttccgcccac
    15481 gcggatgagc gtgaccaact caaatctatt caggccgata tcgccgcaaa agagcgcgcg
    15541 gtacgccaaa agcaacaaca acgcgcaagc ctgctcgcac aattgaaaaa gcaggaagaa
    15601 gcgatctctg aagccacccg taagctgcgc gaaacgcaaa acacgctcaa tcaactcaat
    15661 aaacagattg atgagatgaa cgcgtcgatt gccaaactgg agcagcaaaa agccgcccag
    15721 gagcgcagcc tcgccgcaca actggatgcc gcattccgtc agggcgagca taccggtatt
    15781 cagctgattc tcagcggtga agaaagccag cgtggacagc gtttacaggc ttatttcggc
    15841 tatctcaacc aggcgcgaca agaaaccatt gcccagttga agcaaacgcg tgaagaagtc
    15901 gccatgcagc gtgctgaact ggaagagaaa cagagcgagc aacaaacgct gttatatgag
    15961 cagcgcgccc aacaggcgaa actgactcag gcgctgaacg agcgtaaaaa gacgctggca
    16021 gggctggagt cttccatcca gcaaggtcag caacagttga gcgagctgcg cgccaacgaa
    16081 tcccgtctgc gtaacagcat tgcccgtgcg gaagccgcgg cgaaagcgcg tgcagaacga
    16141 gaagcacgtg aggcccaggc ggttcgcgac cgccagaaag aagcgacgcg caaaggcacc
    16201 acctacaaac cgaccgaaag cgaaaaatcg ctgatgtccc gaactggtgg cctgggggcg
    16261 ccgcgtggtc aggcattctg gccggttcgc gggccgacgc tgcatcgcta tggtgaacag
    16321 ctacagggcg aactacgctg gaaaggaatg gttatcggtg cctctgaagg tactgaagtt
    16381 aaagcgattg ccgatggtcg ggtgattctg gctgactggc tgcaaggtta cggtctggtg
    16441 gtggtggttg agcacggtaa aggcgacatg agtctttacg gctataatca gagcgcactg
    16501 gtgagcgttg gttcgcaggt tcgcgcgggc cagccaattg cactggtggg cagcagtggc
    16561 ggtcagggtc ggccttcact ctatttcgaa attcgccgcc aaggtcaggc ggtcaatcca
    16621 cagccgtggt tgggaagata agttttgttt ccatttcgtc gtaacgttct tgcatttgcc
    16681 gctctgttgg cgctctcctc ccccgtactt gctggcaaac ttgccatcgt cattgatgat
    16741 tttgggtatc gcccgcacaa cgaaaaccag gtgctggcga tgccctccgc tatctccgtc
    16801 gctgtattac ccgattcacc gcacgccaga gagatggcga ccaaagcgca taacagcggg
    16861 cacgaagtgt tgattcatct cccgatggca ccactgagta aacagccgct ggagaaaaat
    16921 acgctgcgcc cggagatgag cagcgacgaa attgagcgca ttattcgtag tgcggtcaat
    16981 aacgtgccct atgccgtggg gatcaacaac cacatgggta gcaagatgac ctctaacctg
    17041 tttggtatgc agaaagtgat gcaggcactg gagcgttaca atctttactt cctcgacagc
    17101 gtaaccatcg gtaataccca ggcgatgcgc gccgcgcaag gtactggcgt gaaggtgatc
    17161 aaacggaagg tgttcctcga cgattcgcag aacgaagcgg acatccgtgt gcaatttaat
    17221 cgcgcaattg atctggcgcg tcgcaacggt tcaaccattg ccattgggca tcctcaccct
    17281 tcaacggtac gcgtgctaca acagatggtt tataacctgc cgccagacat tacgctggtg
    17341 aaagccagca gcttgctgaa tgaaccgcag gttgatactt ctacaccgcc gaaaaacgct
    17401 gtgcctgatg caccgcgtaa tccgttccgt ggcgtgaagc tgtgcaaacc gaagaaaccg
    17461 atagaacctg tttatgctaa tcgcttcttt gaagtattaa gcgaaagcat cagccagagc
    17521 acgctgatcg tttacttcca gcatcagtgg caaggctggg gcaaacagcc cgaagcggcg
    17581 aagtttaacg ctagcgcaaa ttaaaggcgc tacgcgtaat gcgatggtgc gaccgcgttt
    17641 ttttcgcgcc attgccataa gcggaaagac cacagtaacg cctggtaacc gaccttcacg
    17701 ctgcgcacat tggtaatcag gcgcttatac ataccggaag taaatatctc ggcaatcatc
    17761 cgttggcggg taagaatatc cggctctttg cgcaccgcat ggcaaacacg caatgcttcg
    17821 taagttattt gctgatgaaa ttccggataa atcataattt tgtcggcata atttcgattt
    17881 aatttctcca gcaggcgggt aatcttaata tagtgacgtt gataattaag gtttttattc
    17941 ccttgtctat gcaaccgact cactgacg
//
LOCUS       DAZFBR010000097        17594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFBR010000097 DAZFBR010000000
VERSION     DAZFBR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.097"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..162)
                     /locus_tag="ACHJZ2_003603"
     CDS             complement(<1..162)
                     /locus_tag="ACHJZ2_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569643.1"
                     /translation="MRALCAVNGSHPENLRFWLKACPVVLRERFHQQCKCRFNWSTQQ
                     LCENMGLRRF"
     gene            complement(183..464)
                     /locus_tag="ACHJZ2_003604"
     CDS             complement(183..464)
                     /locus_tag="ACHJZ2_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569644.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
     gene            complement(1003..1089)
                     /locus_tag="ACHJZ2_003605"
     tRNA            complement(1003..1089)
                     /locus_tag="ACHJZ2_003605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1053..1055),aa:Leu,seq:taa)
     gene            complement(1102..1175)
                     /locus_tag="ACHJZ2_003606"
     tRNA            complement(1102..1175)
                     /locus_tag="ACHJZ2_003606"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1141..1143),aa:Cys,seq:gca)
     gene            complement(1229..1304)
                     /locus_tag="ACHJZ2_003607"
     tRNA            complement(1229..1304)
                     /locus_tag="ACHJZ2_003607"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1269..1271),aa:Gly,seq:gcc)
     gene            complement(1456..2004)
                     /gene="pgsA"
                     /locus_tag="ACHJZ2_003608"
     CDS             complement(1456..2004)
                     /gene="pgsA"
                     /locus_tag="ACHJZ2_003608"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO4569645.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(2061..3893)
                     /gene="uvrC"
                     /locus_tag="ACHJZ2_003609"
     CDS             complement(2061..3893)
                     /gene="uvrC"
                     /locus_tag="ACHJZ2_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO4569646.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(3890..4546)
                     /gene="uvrY"
                     /locus_tag="ACHJZ2_003610"
     CDS             complement(3890..4546)
                     /gene="uvrY"
                     /locus_tag="ACHJZ2_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO4569647.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4688..4804
                     /locus_tag="ACHJZ2_003611"
     CDS             4688..4804
                     /locus_tag="ACHJZ2_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4569648.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            4842..5018
                     /gene="yecU"
                     /locus_tag="ACHJZ2_003612"
     CDS             4842..5018
                     /gene="yecU"
                     /locus_tag="ACHJZ2_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO4569649.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            5005..5229
                     /gene="yecF"
                     /locus_tag="ACHJZ2_003613"
     CDS             5005..5229
                     /gene="yecF"
                     /locus_tag="ACHJZ2_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO4569650.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(5297..6019)
                     /gene="sdiA"
                     /locus_tag="ACHJZ2_003614"
     CDS             complement(5297..6019)
                     /gene="sdiA"
                     /locus_tag="ACHJZ2_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO4569651.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(6249..7001)
                     /gene="tcyN"
                     /locus_tag="ACHJZ2_003615"
     CDS             complement(6249..7001)
                     /gene="tcyN"
                     /locus_tag="ACHJZ2_003615"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO4569652.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(6998..7666)
                     /gene="tcyL"
                     /locus_tag="ACHJZ2_003616"
     CDS             complement(6998..7666)
                     /gene="tcyL"
                     /locus_tag="ACHJZ2_003616"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO4569653.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(7681..8667)
                     /gene="dcyD"
                     /locus_tag="ACHJZ2_003617"
     CDS             complement(7681..8667)
                     /gene="dcyD"
                     /locus_tag="ACHJZ2_003617"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO4569654.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(8772..9572)
                     /gene="tcyJ"
                     /locus_tag="ACHJZ2_003618"
     CDS             complement(8772..9572)
                     /gene="tcyJ"
                     /locus_tag="ACHJZ2_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4569655.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(9660..10211)
                     /gene="fliZ"
                     /locus_tag="ACHJZ2_003619"
     CDS             complement(9660..10211)
                     /gene="fliZ"
                     /locus_tag="ACHJZ2_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO4569656.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(10257..10976)
                     /gene="fliA"
                     /locus_tag="ACHJZ2_003620"
     CDS             complement(10257..10976)
                     /gene="fliA"
                     /locus_tag="ACHJZ2_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO4569657.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(11296..13053)
                     /locus_tag="ACHJZ2_003621"
     CDS             complement(11296..13053)
                     /locus_tag="ACHJZ2_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO4569658.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            13319..14716
                     /gene="fliD"
                     /locus_tag="ACHJZ2_003622"
     CDS             13319..14716
                     /gene="fliD"
                     /locus_tag="ACHJZ2_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO4569659.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            14741..15151
                     /gene="fliS"
                     /locus_tag="ACHJZ2_003623"
     CDS             14741..15151
                     /gene="fliS"
                     /locus_tag="ACHJZ2_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO4569660.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            15151..15516
                     /gene="fliT"
                     /locus_tag="ACHJZ2_003624"
     CDS             15151..15516
                     /gene="fliT"
                     /locus_tag="ACHJZ2_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO4569661.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            15594..17081
                     /gene="amyA"
                     /locus_tag="ACHJZ2_003625"
     CDS             15594..17081
                     /gene="amyA"
                     /locus_tag="ACHJZ2_003625"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO4569662.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(17115..17528)
                     /gene="yedD"
                     /locus_tag="ACHJZ2_003626"
     CDS             complement(17115..17528)
                     /gene="yedD"
                     /locus_tag="ACHJZ2_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO4569663.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT         4503 a         4532 c         4167 g         4392 t
ORIGIN      
        1 aaacctccgc aaccccatgt tttcacataa ctgttgcgtt gaccaattga atctacactt
       61 acattgttga tgaaatcgct cacgaaggac caccggacag gctttcagcc agaagcgcag
      121 attttcggga tgtgaaccgt taacggcaca taatgctcgc acaacaaacc tgttaaaaga
      181 gattactcag cattctcctg cttccattgc cggatcattt catcggtaac atcaccctca
      241 taacatacca cgtcataccc tccattacgg ctatatgcac tgcgtccacc acatctacta
      301 ccattccttg catgattata aggacacgca caattgcctg gataagattc aatggattct
      361 ttaattattt cttttttgat ctgagcatca gactttcctg ttgctgcata tccactaaaa
      421 gatactaaac aaaggcagat tgccattaat aaaccacatc gcataccgat acactcaatt
      481 actaaccaat catagcccac atcataaaat cgagatgagg agcacaatac ctttaacaag
      541 aaggtgtcat aacgataata tgtcgtgcaa ttcatcaaaa aaaagatcta tatcaaagag
      601 atagaactct tatcagtgct tcagacatcg gcattcgagg cagcagaaaa acagtaaatc
      661 tgacgaattt gccttttccc aagatgttgt cagattaaaa agatagatta gcgcaggtac
      721 ctgttggtgg attgcaggag gaaaaataaa gtcgagtgga aaaatcccag aaaaattttt
      781 gtttaacaga ggtcctggtt cactatgcga aatacggagg agctgcagaa gaattctgct
      841 tcatccttag agtttaaaca gcacgtgcac taccattgtt tctgcatatc gccacaccat
      901 cgccactcta ccgccattgc acaaaataca aatacgaaaa aaccacccga aggtggtttc
      961 acgacactgc ttattgcttt gattttattc ttagctttcc catggtaccc ggagcgggac
     1021 ttgaacccgc acagcgcgaa cgccgaggga ttttaaatcc cttgtgtcta ccgattccac
     1081 catccgggct cgggaagaaa gtggaggcgc gttccggagt cgaaccggac tagacggatt
     1141 tgcaatccgc tacataaccg ctttgttaac gcgccaaatt cttcaggcct ttcagccaga
     1201 catccgctga cgccgatgtc ttttaaactg gagcgggaaa cgagactcga actcgcgacc
     1261 ccgaccttgg caaggtcgtg ctctaccaac tgagctattc ccgcattcat caagcaatca
     1321 gttaatcact tgattttatt atcgtctggc aatcagtgcc gccgttcgat gcgttgcatt
     1381 ctacttacct ggcgcgatga gtcaacgata tttttcacca cttttgatcg tttgctgaaa
     1441 attgcgccga aacgatcact gatcaagcaa atctgcacgc gcagcgctca aatattgcaa
     1501 cattgaccac agagtcagta ccgcagccac aaagaaaagt gcaataccgg cgtactcaac
     1561 ccaaatgttc ggacgccaca gcagccatgc caacgccacc atctgggcag tggttttcac
     1621 tttcccaatc caggagacag ccacgctact gcgtttaccc aactccgcca tccattcgcg
     1681 tagcgcagaa ataataattt cacgggcgat catcgttgcc gccggtaagg tcacccacca
     1741 gctgtggtaa tgctcggtta ccagcaccat ggcgatagcc acgagaactt tatctgctac
     1801 agggtcaagg aaagcaccaa accgggtact ctggttccag cggcgtgcca gaaaaccatc
     1861 gaaccagtca gtcaccgccg cgacgcagaa aatgagcgcg gcggcaaacg gcgaccaagt
     1921 gacaggcaga taaaagacca atacaaagaa tgggataagg atgacacgga acagtgtaag
     1981 caacgtaggg atattaaatt gcataatgac gggtaactat ctgttgtcag taagattacc
     2041 cctatgttgc tacagagaca tcaatgtttc aacgaccaga agatcttttc tgccagacct
     2101 tgcgaaatac ccggcacttt tgcaatttcc tcgacgctgg cgttacgtaa accttgcaaa
     2161 ccgcccatat atttcaacaa catttgccgg cgttttggcc cgacgccttc aatggtttcc
     2221 agggaactgg tatttttgac cttcgcccgt tttttacggt gcccgccaat cgcgtgatcg
     2281 tgtgattcat cgcgaatatg ctggataaca tgcagcgcgg gagaatctgg cggcaaactg
     2341 aatccctcac cttccggctc aaagaacagc gtttccagcc cagccttacg atctgctcct
     2401 ttggcaacac caagtagcag cggatgattt ttatcccatg agacatccag ttcggcgaag
     2461 acatttttcg cctgcgcaag ctggcctttg ccgccgtcga taagtatcac atccgggatc
     2521 ttactgtcgt cgatggcttt accataacgc cgacgcagca cctgattcat cgccgcataa
     2581 tcatcgcccg gcgtgatgcc agtaatgtta tagcgccgat actccgcacg cagcgggccg
     2641 ttagcatcaa acaccacaca ggaagcgacg gtttgttcac ccatggtatg gctgatgtca
     2701 aagcactcca tccgcttcac ttccggcaat ttcaacacgc tggcaagtgc tgtaagccgc
     2761 tggtgaacgg tagattgctg cgaaagtttg ctggttaagg ccgtcgccgc attggtgcgc
     2821 gcgagtttca ggtaacgcgc cctatcgcca cgaggtttgg tttgaacatt aatcttgcgt
     2881 cccgccagtt ctgaaaggga atcggcgagc agcgttttat cgctaagatt aaaatcgagc
     2941 aggatctcac ccggtaaggt gcgcatctgg ctgccttgta aatagaactg gcctacgaag
     3001 gtttctacca cctcgctcag ttccgtaccg ccaggcactt tcgggaaata gctgcggctg
     3061 ccgagcactt tgccctgacg aatgaacaat acgtggacac aagccatacc cgcatcgaac
     3121 gccacaccaa taacgtcgag gtcgtcgccg gtattggaaa cgaattgttt ttcggtgacg
     3181 cgtcgcaccg cctgaatttg gtcgcgaata cgtgccgctt cttcaaactc cagattctga
     3241 ctggcagttt ccatacggct aatgagttgc gtaagcacct gatcatcttt gccagacaaa
     3301 aacaggcgca catactcgac ctgctgagcg tattcttctt cactcaccag tccttcaacg
     3361 cacggtccca gacagcgccc tatctgatat tgcagacacg gacgcgagcg attgcgataa
     3421 acactatttt cgcactggcg aatggggaaa atcttttgca gtagcgccag tgtttcacgt
     3481 acggcatagc cattcgggaa cgggccgaaa tattcacctt tggcatgctt tgcaccacga
     3541 tgcatcgcca gacgcgggtg ggtatcgcca ctcaggaaga taaaaggata tgatttatca
     3601 tcgcgtagca aaacgttgta acgcggctga tagagtttga tgtagttgtg ttccagcaac
     3661 agcgcttcgg tttctgtatg agtaaccgtt acatcaattt gctggatctg ggcgaccagc
     3721 gcttcggttt tgcgcgaagc gaggttgcta cggaaatagc tggaaagccg ttttttcagg
     3781 tctttcgctt tgccgacata gataaccgta ccaccagcat cgtacatgcg ataaacgcca
     3841 ggctggctgg ttacggtttt taaaaacgct tttgcgtcaa actgatcact cactgacttg
     3901 ataatgtctc cgcattacac agaccatggc gaattgccag gtgagtcagc tcaacatcgc
     3961 catgaatgtt tagtttactg aacatacgat agcggtagct gttcaccgtt ttcggactga
     4021 gattgagctg ttctgagatc tcattgacct tctggccctt cgtgatcatc agcataatct
     4081 gcaattcacg ttcagacaaa ctggcaaatg ggctttctgt tttttctggt tcgatctggc
     4141 ttaatgccat ttgttgagcg atgtcagaag caatgtaacg ctgcccagaa tagacagaac
     4201 ggatcgcact cacgacttcc tgcggagccg cgcctttgct gaggtagccc gcagcaccgg
     4261 cctgcatgac tttcgctggt aaagggtttt ctgtatggac ggtaagcatg atgattttta
     4321 catcagctgt ggaacgcgcg attttacgcg tcgcctcaag accgccaatg cccggcatac
     4381 tcatgtccat tagcaccacg tcaacggcat ttgcccggca ccacttaacg gcgtcttcac
     4441 cgcacgatgc ctcaccgacg acttttatac cctttatatc ttccagaatg cgtcgtatcc
     4501 ctgcgcgcac cagttcgtgg tcatcaacaa gtagaacgtt gatcaaagga atatctccag
     4561 aaatagggat aacgctactg atagttagtc attcgtatat tagcggtttt tattgcaact
     4621 ttgaaacgtt aaaaatggtg ggtattcgat ttttctctcg tttttgtcaa ttcgattgtc
     4681 cacagttatg gtcacgcccg tcaccaacgg cttaccggat cgtttcacag taagccctgc
     4741 gacgaaatgt gacaaaaatt acctttattc agcaaaaatg aaaatcagcc cgctgaatat
     4801 gtaacattaa ttaacccgaa caatacgggc aaaaaacatt catgataaaa atatttattg
     4861 gtcattatat taacgcattt aatagcaccg ttgacggcac gctcaaaaaa caaccactgc
     4921 tatttttagc aaagcttatg gtatactccg ccgccttaac atttttcacc gcaaattttc
     4981 attgcaacat gacgaggaaa ataaatgagt acgcctgatt tttctactgc cgagaataat
     5041 caagaactgg caaatgaagt ctcctgcctg aaagcgatgc tgacgctgat gctgcaggcg
     5101 atgggacaag ctgacgcggg ccgcgtgatg ttaaagatgg aacaacagct tgcgctgatc
     5161 gaagacgaaa cccaggctgc agtattttcc aaaacggtta agcaaattaa acaagcctac
     5221 cgtcagtaat ataaaaccgg ctgatagcgt gcctttcagc cggtttttgc atctggcacg
     5281 caggacagaa aagagatcaa attaagccag tagcggccgc gtaacaggca acctgggtct
     5341 tatttggtgc attaattttt ttctgcatgt ttttctggtg gaaattgacc gtattctcag
     5401 agattgacaa aatcatcgct atctctgctg atgtcttccc ttccgccgtc cacctcagaa
     5461 tttctttttc gcgcttgctg aaattcatct ctggcgtcat cactatttca tcatttaaac
     5521 gcatcagagc catcagactt tcgcgcacca gtaactgcat ttttaattgc agttcatcac
     5581 taagaatggg tatttcgcgc gcgctgcaac gggaaaagga caaaaagccc agcgcccggt
     5641 ttggcagcat taaatactga gtgacaccgc ggcgtaaacc atgtgcgcgc gcggcttccc
     5701 ataacggctg tgcttcgttg aataagtcat cattccacat tagatggccc tgactaaagt
     5761 tttcagggtt cagcaccgga tcaattgcga gaaagttttt tgcctgataa taactaaccc
     5821 acgcctcagg gtaattggtg tagaaagcca ctttaggtcg agtgaatggt accgggtggc
     5881 ggacacataa cgaatagtaa tcgtactcca gctgctgagc ctgaagctca atttcatggt
     5941 agacctcttc tgcggtctcc atcctctgaa aacgcaacag catcgtgcga cgccagctga
     6001 aaaaatccgt atcctgcata gtaaaccgca acgcccctga gagtgagtat catttataat
     6061 gataattcaa actatcacat aaataagatt tatatataat ttatattatt caggcaatga
     6121 attacttttg caagccatcg cattctctta tgttattaat gagttatgct gattttttaa
     6181 gcagttttat caggcttgaa atggcgttcc gccccgactg tcgaatcgtc ggggctgatt
     6241 ttttcttatt attgcagcag aaacttctcg aggaactggc gggtgcgagg ctgctcggga
     6301 tcggcaaata acgcttttgc ggccccctgc tcgactatcc gcccctggtc cataaaaatc
     6361 gcccggtccg caacatcccg ggcaaagctc atttcgtgtg tcacaatcac catcgtgcgc
     6421 ttttcctgcg ccagctgacg gatggtgttc aggacttcgc ccaccagctc tggatccagc
     6481 gctgacgttg gctcgtcaaa caaaatcacc tcaggacgca ttgccagcgc acgcgcaatc
     6541 gcaacacgct gctgttgacc gccagacaaa cgacgtggat agctggtttc tttaccggcc
     6601 agcccaactt tcgccaacag cttgcgagcg cgcgccgtgg cctcttcttt cggttcgcct
     6661 ttgacgatca ccggcccttc aataatgttc tccagcaccg tacgatgcgg aaacaaatta
     6721 aagttctgga agacaaaccc gacgtgctga cgcaactggc gaatcagcga tttttgctga
     6781 cttaatgaac gtgcagtatc aatagtgata tcgccaacgg tgatcgttcc cgcttccggt
     6841 tgttccagca gatttatgct acgtagcaat gtggttttgc cggaaccact cggaccgata
     6901 attgccacca cttcgccagg ctttacctcg aggtcgatac cgtgcagcac cgtctgaccg
     6961 tggaattttt tcactaggtt cttaacttca atggcactca tttgggttct ctctcctggc
     7021 gattaagttg attctcaaaa tggttctgca acgtcgataa cacagtcgcc atgatccagt
     7081 agatgagcga agccgccaga tacatggtga atacctccag cgtacgcgag gtaatcaact
     7141 gcgcctgacg gaacagctcc ggcacctgga ttgtcgcggc cagcgacgta tcttttacca
     7201 gactgataaa gctgttcgag agcggcggca acgccacccg cgccgcctgt ggcaaaatcg
     7261 cgcgacgcat agtctgccat ggcgtcatac caatactggc agctgcttcc cactgacctt
     7321 tatcaataga agaaatggcg gcgcgaagcg tttcggcagt ataggcggca gtgttgagcg
     7381 acagaccaat cattgctgac ggaatgggat ctaattcgat accaaactgc ggcaggccgt
     7441 aatagatcat aaacagttgt gcgatgagcg gcgtaccacg aaaaatagag atataaaaac
     7501 gcgccagcca gcgcacgggc cagattggcg acaggcgcat cagcgcgaga ataaacccta
     7561 gcagtaaacc aaaaaacatg ccgccgatac tgagttgcag cgtataccca gcccctttca
     7621 gcaggaacgg caaggaatca ataaccagtt gtatactttc ttgcatgagc gttcccggag
     7681 ctaaacgtgg ggatgatagg cgaacagcgc aggcgcgccg ccggtatgaa taaacagaat
     7741 cggcccttca tctttgaagc gtttctgact gataccgtca atcagacccg ccatcgcttt
     7801 tccggtatac acaggatcaa gcagaatgcc ttccagccgc gccagcaatt tcactgcttc
     7861 catgccttcg tcgttcggca cgccgtagcc aggtgcaaaa tagtcatccc agagtaaaat
     7921 ttccactgat gcggtcagct ccagttcttt cgcaatcgcc tgttgtaggt taaccacttt
     7981 cggcaattga tcggcaacgg aacgcgacac ggtcacgcca atcagttcgc tttcaggcat
     8041 caggtgttcc agcccaacag ccagtccggc gtgagttccg gcactgcccg atgcgactac
     8101 caccgatgaa atattaaccg ccccttcaca ctgttgcgcg atttccagcg cactctccac
     8161 ataacctagc gcgccaagag cattagaacc gccaaccgga atgacatacg ggcgaaagcc
     8221 ttgtgcttcg actcgcgtcg ccagctcttc cagttgggca ttgggatcgg tcagtgcgtc
     8281 gcacatttca atctgggtat tgaacagatc cagcaacaag cgattgccgt tggttaaata
     8341 gttttctgcg gttgtgccaa taggattttc cagcagcgcc acgcagtgca gaccgagttt
     8401 ggcagcgact gcggcagtct gccgcacatg gttagactgg atcgccccgg cagtaatcag
     8461 cgtatcggca ccttcacgca gagcatctgc cgcgagaaat tctagcttac gtaatttatt
     8521 gccgcccatt gccatggggg tgacgtcatc ccgtttgatg aaaatttccc gtcctagata
     8581 atcagaaaag cgcggcagat attcgagcgg cgttggcgcg ccgataaact ccagccgtgg
     8641 aaaacgggtt aaattatgca gtggcataac agcctccgat gtgtgttgtt gtgattttct
     8701 cattatgcac gctgaaaacg cgtaaataaa aaaggcgcta gtgaaagcgc ccttttttgt
     8761 cattatgctg attatttggt cacatcagca ccaaaccatt tttcggaaag ggcttgcaaa
     8821 gtgccgtctt tttgcatttc cgcaattgca tcattcactg ctttcagcag gtcttcattt
     8881 cctttacgca gcgccacgcc agactcctga cgggagaatg cttcaccggt tactgccagc
     8941 gtatcgttgg ttttcttcac cagatccagc gccgccagac gatcaacgag gatcgcatcg
     9001 atacgcccta cgcgcagatc ctgatatttg gtcgggtcat catcataggt acgcacatcg
     9061 acgccctgaa cattctgccg cagccactct tcatagttgg tgcccagacc gacccccact
     9121 tttttgcctt tcagatcatc ggctgtttta atggtgcctt cgttaccttt tttcaccagc
     9181 gcctgaatac cagaaatggt gtacggggtt gagaaatcgt attttttctt gcgctcatca
     9241 gaaatggtga cctgattaat caccacatca atacgtttag agtccagcga cgccagcata
     9301 ccgtcccatt tggtcggttt tagtgacgcc tcaacgccaa gatgttttgc cagctgttgg
     9361 gcaaattcca cttcaaaacc ggttaatttg ccgtcatccc cctgaaaact gaacggcgga
     9421 taagtacctt ccagccctac cagcagcgtg ccgcgctctt taactttatt aagcagacct
     9481 tcatctgcaa aacttttaac gctcatgccc gcaatcagcg ctacggccat cacacccatc
     9541 aatgcctgac gtcccagatg tgctaatttc atattcaccc cgaatgttgt tatgtctgtt
     9601 tgcagtgtag agccattgtt tgtaaacaca aaaacaactc cgctacatct tattcttatt
     9661 taatatatat cagaagcagg caggctggag gatttctgca caagtccggt acaggtttgg
     9721 cgctgatagt gttgatactt ccgtaacgcg atgcggtaat tgttggtgct ggtggtgggc
     9781 agccacgggg cgaggatttc atcaaggaaa ccgtcctgca gctgatcgag ggaaatattt
     9841 tgctcgtgta gatgattccc caaacggcgc agacgaacga catattcacg caccgtacca
     9901 gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc gaaaaaatca
     9961 ctctgcgttt tgcaatgtaa atcaccgcaa aatcggcaca atgccgccca tgatttttgt
    10021 tccgtttgcc agccattttc atcaagcagc gtgtccaggc gggaaatctc gattttattc
    10081 actattttgc cgtcgcgaac taaggtaatg cgatcgagca atttacggca atgcgcgcaa
    10141 tgggtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct tttcaggtgc
    10201 tgcaccatca ttaagaactc ctggtagtca aagttaaagt gcggcattta ctgacgttat
    10261 aacttaccca gtttagtgcg taaccgttta atagcctggc tgtgtaactg actgacccgc
    10321 gattccccga cctccagcac tgcaccaatc tctttgagat tcagctcttc ctggtaatag
    10381 agggttaata ccagtttttc gcgctccggc aacgtttcga tagcttccat cacccgctgg
    10441 cgcagattac tgtccagtag ttgttgtagc gggttttctc gttgatgatc atcagtaacc
    10501 aattcgatgc tatcgccgtg ctcttcgcgc cactcatcgt aggagaagag ctggctgtta
    10561 ttggtgtcga gcaacatttg gcgataatcg gcaatatcga tccctaaacg ttccgctacc
    10621 tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc ctgtgccact
    10681 tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag ttcatccagc
    10741 atagcgccac ggatacgctg cactgcgtaa gttgtaaatg ccgttccttg tagggcgtca
    10801 tagcgttcga cggcattaag taacccaatg ccgcccgcct gtagcagatc gtcaagttcc
    10861 acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag cgggacataa
    10921 cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga attcacgata
    10981 aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg gtttttaatc
    11041 ggacgattag tgggtgaaat gaggggttat ttgggggtta caggtaaatt ccaggcagaa
    11101 aaaaccccgc cggtggcggg gaagcacgtt gctgacaaat tgcgctttat gttgccggat
    11161 gcggcgtaaa cgccttatcc ggcctacaag aatgtgcaaa ttcaataaat tgcaattcaa
    11221 cttgtaggcc tgataagcgc agcgcatcag gcaatttggc gttgccgtca gtctcagtta
    11281 atcaggttac aacgattaac cctgcagcag agacagaacc tgctgcggta cctggttagc
    11341 ttttgccagc acggagttac cggcctgctg aatgatctgc gctttcgaca tgttggacac
    11401 ttcggtcgca tagtcggcgt cctgaatacg ggactgcgct tcggacaggt tggtagtggt
    11461 gttgttcagg ttggtgactg cggaatccag acggttctgg atagcaccca gggaagaacg
    11521 gaatttgtcg atggagctga ttgcgtcgtc cagggcagca agcgggttgg tcgttgcaga
    11581 accagcactg gtagtctcag tagtgatttt acccgcggag ttcacattta cagcagtacc
    11641 aattgctgga gcataatctt tattggtatc agtcgctgaa gcatacccgg caacagtcac
    11701 agagccgtta tccttgttaa cgctgtaaga gactgtatag tcggcaacgt tagtgatacc
    11761 acctttatca tccacataag acttcgcagc atcactggat tcacccgcgg taaacccaat
    11821 agttttgctc agtacaccgg aattaaaggt aattgaagat gtcgcggcag ccgctttggt
    11881 ttcgctcaat actacatctt tactcactgt agcctgataa gtaatcccac cagggattaa
    11941 cggagctact ggagtggttg tcgcaggagt tgcttttgcg atggtatatt ctgtgccatt
    12001 gaatgtcaga gaggcaccgt cactaccttc gctcgctgct ttgagcagcg ctttcatatc
    12061 agctttagct gcgctaccag cgttgttagt cgtcaagttg cctgcatcgt ctacgtatgc
    12121 ctggcttcca ccgatggtga tattacctgc tgaatccgtt tcgaaagaaa cagtaccatc
    12181 ttttgtggtg taagaaccat taacagattt acccacatca gcatgaaggg tggcagctaa
    12241 cgcatcacgt tttgaagctg aatcagcagc ctgcgccgcg gcgtctacac ccgtaccagc
    12301 agtagattta gtggtagtaa aatcaccaga tttagcgttg taagtataat ctacgccgcc
    12361 aactgtgact ttatcgccat tccctaattt atcgaatgca gcatcggtag ttaacaaggt
    12421 attttcggtt ttcagatcat aaagacctgt cgtggtgttt aacttcgcgc cagcagaagt
    12481 taaatcactt accgttgcag ctttgttggt aatagtacct ttaccattta cgttaaagcc
    12541 attcagaccc agagtatcag aatcgatttt tttcaggtcg atcgtgatgg tttcgccgtc
    12601 attcgcacca acctgaattt tcattgaacc gtctttcgcc agcacgttca cgccgttgaa
    12661 ctgggtctgg ccggatacgc ggtcaatttc atcaagacga gatttgattt cgtcctggat
    12721 ggagtccagg tcagaatcgg agttagtccc tgtagtggcc tgaaccgtca gttcacgaat
    12781 acgctgtaag ttgttgttga tttcggacag cgcgccttcg gtggtctgcg caacagaaat
    12841 accgtcgttg gcgttacggg ccgcctgagt caggccttta atgttagaag taaaacggtt
    12901 agcaatcgcc tgacctgcgg cgtcatcctt cgcgctgtta atacgcaagc cagaagacag
    12961 acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt gagtgatcag
    13021 cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat tgcaagtcgt
    13081 tgattacgta ttgggtttcc acccgtcggc tcaatcgccg tcaaacctgt tatcgtctgt
    13141 cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt cgccgaaata
    13201 ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta aactttgcgc
    13261 aattcaggcc gataaccccg gtattcgttt tacgtgtcga atgataaaag gaaatcgcat
    13321 ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa tccttgatag
    13381 cctcaccgcc gcgcaaaaag cgacgctaac ccccatttca aatcagcaat cgtcgtttac
    13441 cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc agaccgccaa
    13501 tactgcattg tctaaagccg atcttttttc cgccaccagc agcaccaccg cgttcagtgc
    13561 caccaccgcg ggtaacgcca tcgccgggaa atacaccatc agcgtcaccc atctggcgca
    13621 ggcgcaaacc ctgaccacgc gcaccaccag agacgatacg aaaacggcga tcgccaccag
    13681 cgacagtaaa ctcaccattc aacaaggcgg cgacaaagat ccgattacca ttgatatcag
    13741 cgcggctaac tcgtcgttaa gcgggatccg tgatgccatc aacaacgcaa aagcaggtgt
    13801 tagcgcgagc attattaacg tgggtaacgg tgattatcgt ctgtcagtca catcaaatga
    13861 caccggcctt gacaatgcga tgacgctctc ggttagcggt gatgatgcgc tacaaagttt
    13921 tatgggctat gacgccagtg ccagcagcaa cggcatggag gtctcggtcg ccgcccagaa
    13981 tgcgcagctg acggtcaaca acgtcgccat tgagaacagc agcaacacca tcagcgacgc
    14041 gctggaaaac atcaccctga acctgaacga tgtcaccacg ggcaaccaga cgctaaccat
    14101 cactcaggac acctccaaag cgcaaacggc gattaaagat tgggtgaatg cctataactc
    14161 gctaatagat accttcagca gcctgaccaa atacaccgcc gtagatgcgg gagctgatag
    14221 ccagagttct agcaatggcg cactgctcgg cgactccacg ctgcggacga ttcagacgca
    14281 gttgaagtcg atgctgagta ataccgtcag ttcttccagc tataaaacgt tggcgcagat
    14341 tggtatcacg accgatccca gcgatggcaa actggaactg gatgccgaca aactcaccgc
    14401 tgcactgaaa aaagatgcca gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac
    14461 cggcatcacg accaccatcg gcagcaacct gaccagttgg ctttcgacaa cgggcattat
    14521 taaagccgct accgatggcg ttagtaagac cctgaataaa ttaactaaag actacaacgc
    14581 cgccagcgat cgcattgacg cgcaggtcgc gcgctacaaa gaacaattta cccaactgga
    14641 cgttttaatg acctcgttaa acagcacaag cagctactta acgcagcagt tcgaaaacaa
    14701 cagtaattcc aagtaatcat tattcatcgg gagacaggtc atgtacgcgg caaaaggcac
    14761 ccaagcctat gcacaaattg gcgtcgaaag cgccgtaatg agcgccagcc agcagcagct
    14821 ggtcaccatg ctatttgatg gagtgctgag cgcactggtt agagcgcgcc tgtttatgca
    14881 ggacaacaat cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa
    14941 cggactgcgg gtgagtcttg atgaagagag caaagacgaa ctaacccaaa acttgattgc
    15001 tctttatagc tatatggtca ggcgtttgct gcaagccaat ttacgcaacg atgtctccgc
    15061 agtcgaagaa gtggaaacat taatgcgcaa tattgccgat gcctggaaag agtcgttact
    15121 ctccccttct ttgattcagg acccagtctg atgaaccatg caccgcattt atatttcgcc
    15181 tggcaacaac tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg
    15241 gacgaactca tcgccagcga aatggcgtat gttaatgcgg tgcaggagat tgcacatttg
    15301 actgaagagg ttgacccgtc caccacgatg caggagcagc tccgcccgat gctgcgcctg
    15361 attctcgaca acgaaagcaa ggtaaagcag ttattacaga ttcggatgga tgaactggcg
    15421 aaactggtcg gtcagtcatc ggtgcaaaaa tcggtgttga gtgcctatgg cgatcagggc
    15481 ggctttgtgc tggctccgca ggataacctc ttttgaatct gaatgagtcg atggctcgcg
    15541 aataatccga ttacggctac gcttctaatg ttccccttga atggagtcga agaatgcgta
    15601 atcccacgct gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg
    15661 aattggccga gcgcgccgac ggttttaatg atattggtat caatatggtc tggttgccgc
    15721 ccgcctataa aggcgcatcg ggcgggtatt cggtcggcta cgactcctat gatttatttg
    15781 atttaggtga gtttgatcag aaaggcagca tccctactaa atatggcgat aaagtacaac
    15841 tgctggccgc cattgacgcc ctgaaacgta atgacattgc ggtgctgttg gacgtggtag
    15901 tcaaccacaa aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgc gttaatgctg
    15961 atgaccgtac gcaaattgat gaagaaatca ttgaatgcga aggctggacg cgttacacct
    16021 tccccgctcg tgccgggcaa tactcacagt ttatctggga tttcaaatgt tttagcggta
    16081 tcgaccatat cgaaaacccc aatgaagatg gcatttttaa aattgttaac gactacaccg
    16141 gcgaaggctg gaacgatcag gttgatgatg aactcggtaa tttcgattat ctgatgggcg
    16201 agaatatcga ttttcgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg
    16261 tgatggaaca aacgcaatgc gacggttttc gtcttgatgc ggtcaaacat attccagcct
    16321 ggttttataa agagtggatc gaacacgtac aggaagttgc gccaaagccg ctgtttattg
    16381 tggcggagta ctggtcgcat gaagttgata agctgcaaac gtatattgat caggtggaag
    16441 gcaaaaccat gctgtttgat gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg
    16501 gacgcaacta cgacatgacg cagattttca ctgggacgct ggtggaagcc gatccttttc
    16561 acgccgtaac cctcgttgcc aaccacgaca cccaaccgtt gcaagccctc gaagcaccgg
    16621 tcgaaccgtg gtttaaaccg ctggcgtatg ctctaatttt gttgcgggaa aatggcgttc
    16681 cttcggtgtt ctatccggac ctctacggtg cgcattacga agatgtcggt ggtgacgggc
    16741 aaacctatcc gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc
    16801 agcgtttcgc ccacggtgta cagacgttat ttttcgacca tccgaactgc attgccttta
    16861 gccgcagtgg caccgacgaa tatcccggct gcgtggtggt catgtcgaac ggagatgatg
    16921 gcgaaaaaac cattcattta ggagagaatt acggcaataa aacctggcgt gattttcttg
    16981 ggaaccggca agagagtgta gtaaccgacg aaaacggcga agcgaccttc ttttgcaacg
    17041 gcggcagcgt tagcgtgtgg gttatcgaag aggtgcttta aattcatccc cggcggcaag
    17101 ccggggagat ttcattacgg caatggcgtc ggtaacggcg ctttatccag cgcagcggca
    17161 cactctgccg tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta
    17221 ccgtcacgct caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatcagac
    17281 atcaatgtca acttgcccgg cacagcaatc acccgctgcc actgacggca atctagggta
    17341 tcgccctctt tagtgacaat caggctgcct atcgcttccg ggctgaccag cgcacgttgc
    17401 ggccctttgg tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg
    17461 ttatagtttt cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt
    17521 tttttcatca ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gccctgatgt
    17581 cgccagcctt tcca
//
LOCUS       DAZFBR010000098        17390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFBR010000098 DAZFBR010000000
VERSION     DAZFBR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.098"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(558..1754)
                     /gene="hemY"
                     /locus_tag="ACHJZ2_003627"
     CDS             complement(558..1754)
                     /gene="hemY"
                     /locus_tag="ACHJZ2_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO4569664.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1757..2974)
                     /gene="hemX"
                     /locus_tag="ACHJZ2_003628"
     CDS             complement(1757..2974)
                     /gene="hemX"
                     /locus_tag="ACHJZ2_003628"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO4569665.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQADTPAAAPQGE"
     gene            complement(2996..3736)
                     /gene="hemD"
                     /locus_tag="ACHJZ2_003629"
     CDS             complement(2996..3736)
                     /gene="hemD"
                     /locus_tag="ACHJZ2_003629"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO4569666.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3733..4674)
                     /gene="hemC"
                     /locus_tag="ACHJZ2_003630"
     CDS             complement(3733..4674)
                     /gene="hemC"
                     /locus_tag="ACHJZ2_003630"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO4569667.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            5061..7607
                     /gene="cyaA"
                     /locus_tag="ACHJZ2_003631"
     CDS             5061..7607
                     /gene="cyaA"
                     /locus_tag="ACHJZ2_003631"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO4569668.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7647..7967)
                     /gene="cyaY"
                     /locus_tag="ACHJZ2_003632"
     CDS             complement(7647..7967)
                     /gene="cyaY"
                     /locus_tag="ACHJZ2_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO4569669.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(8129..8271)
                     /locus_tag="ACHJZ2_003633"
                     /pseudo
     CDS             complement(8129..8271)
                     /locus_tag="ACHJZ2_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8430..8633
                     /gene="yifL"
                     /locus_tag="ACHJZ2_003634"
     CDS             8430..8633
                     /gene="yifL"
                     /locus_tag="ACHJZ2_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4569670.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8670..9494
                     /gene="dapF"
                     /locus_tag="ACHJZ2_003635"
     CDS             8670..9494
                     /gene="dapF"
                     /locus_tag="ACHJZ2_003635"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO4569671.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9491..10198
                     /gene="yigA"
                     /locus_tag="ACHJZ2_003636"
     CDS             9491..10198
                     /gene="yigA"
                     /locus_tag="ACHJZ2_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO4569672.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10195..11091
                     /gene="xerC"
                     /locus_tag="ACHJZ2_003637"
     CDS             10195..11091
                     /gene="xerC"
                     /locus_tag="ACHJZ2_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO4569673.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            11091..11807
                     /gene="yigB"
                     /locus_tag="ACHJZ2_003638"
     CDS             11091..11807
                     /gene="yigB"
                     /locus_tag="ACHJZ2_003638"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO4569674.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11891..14053
                     /gene="uvrD"
                     /locus_tag="ACHJZ2_003639"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11891..14053
                     /gene="uvrD"
                     /locus_tag="ACHJZ2_003639"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO4569675.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(14098..15000)
                     /locus_tag="ACHJZ2_003640"
     CDS             complement(14098..15000)
                     /locus_tag="ACHJZ2_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HFO4569676.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(15083..15847)
                     /gene="yigE"
                     /locus_tag="ACHJZ2_003641"
     CDS             complement(15083..15847)
                     /gene="yigE"
                     /locus_tag="ACHJZ2_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO4569677.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            16009..16065
                     /gene="ysgD"
                     /locus_tag="ACHJZ2_003642"
     CDS             16009..16065
                     /gene="ysgD"
                     /locus_tag="ACHJZ2_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO4569678.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            16217..17167
                     /gene="corA"
                     /locus_tag="ACHJZ2_003643"
     CDS             16217..17167
                     /gene="corA"
                     /locus_tag="ACHJZ2_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO4569679.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17192..17389)
                     /locus_tag="ACHJZ2_003644"
     CDS             complement(17192..17389)
                     /locus_tag="ACHJZ2_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569680.1"
                     /translation="MRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAW
                     ILFTTIMLSAIPSAKMSLSAH"
BASE COUNT         3984 a         4531 c         4629 g         4246 t
ORIGIN      
        1 tccaagaaat gaatttaaaa atcaggagag cgttttcaat cctacctctg gcgcagttga
       61 tatgtaaggc aggtttatta tatcgcgttg attattgatg ctgtttttag ttttaacggc
      121 aattaatata tgtgttatta attgaatgaa ttttatcatt cataataagt atgtgtagga
      181 tcaagctcag gttaaatatt cactcaggaa gttattactc aggaagcaaa gaggattaca
      241 gaattatctc ataacaagtg ttaagggatg ttatttcccg attctctgtg gcataataaa
      301 cgagtagatg ctcattccat ctcttatgtt cgccttagtg cctcataaac tccggaatga
      361 cgcagagccg tttacggtgc ttatcgtcca ctgacagatg tcgcttatgc ctcatcagac
      421 accatggaca caacgttgag tgaagcaccc acttgttgtc atacagacct gttttaacgc
      481 ctgctccgta ataagagcag gcgttttttt atgtgtcagg aaggtcccgg aggtgcttgc
      541 ctccgggtga gaaggaacta ctgtggtggg ttgttctgca acgttaacat caaaccgtcg
      601 cgacgcatcg ctgcagcttc ttccggcttg tgcagtctgt ccagcgcgtc ggcaagccat
      661 gcgtaatcgt aggcgtccgg acgttgtttc agcgctgcgc ggaaggcgag cgatgcttcc
      721 tgccattctc cgtgcttcat cagtgactgg cccagtgtgc tccacaacag cgggcgatcg
      781 ccgacgtttt tgatttgctg gcgcagcact ttttccagct gttccggatt gtttgttttc
      841 agtcgaggaa tcggcagcag taggcgatcg tcgtactggc gtttcaggcc atcgatgata
      901 atttgctggg cagtatcatg atcgtcacat tcaataagat gttccgccat tgccacctgc
      961 aacgctacct gatgacgcgt tttccggctt tggtttttcc accagttacg caaaccttcg
     1021 ctaccgttat cggcacgcgc ctgatccatc aggccaatcc atgcctgttg ttccagcatt
     1081 gcacgatgtt cttcatcacc aacatgggct ttcgccattg atgggataat atccagcagc
     1141 gaactccatg cacctgtgcg gatatacgcc tgttccgcca gacgtaatac ttccggatgg
     1201 cgtggcgtaa cttccagcag cttatccacg ccgtggcgtg cagcatggtt ttcattacgg
     1261 gccagttgca gacgtacacg ggtgatttct accggaatgg tgtcattgcc ggccagctcc
     1321 gctgcgcgtt ccagatgttg gttggcgcgc gcttcatcac cacgttgttg cgcggcttcg
     1381 gcagccagta gatagttcac caccggttgt tcagcgtgat cggcattttt cgccatcagc
     1441 ttttcaactt gctgataatc gccttccgcc agtttcagca gcgcctgttc ggtctgctta
     1501 cgtgcacggc gacgcttacg cccgacaaac cacccacggg tgtgcgcgcc agtgcggaag
     1561 atccgccgca gtagccactc aatggcaaac agcactacca tcgccagaat caatatgatc
     1621 gccaggcccg tgacgctggt ttcgatattg tagttgtcgg tctggatcag cacataaccc
     1681 tgatgaccgg caatcatcgg gccaaccacg atccccgcaa tcagcaacac aaagagcaat
     1741 aacactttta gcatggttat tctccttgcg gcgcggctgc cggagtatca gcttgcggtg
     1801 caggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg tgcaggttta gcttccgttg
     1861 tccccgctgc cggttgtgcc agcaggttac gcacgcgagt ctgcatcagt ttttccagca
     1921 tcgcctggct ttgcagggtt tccggaagat ccatcgagat attttgctgg cttaactggt
     1981 ctacctcgtc gaggaacgct ttggtggtgg catcatcagt atcgtagtaa gcacgtaccc
     2041 aggtggagac gttctccagc gcctggcgat aagtctcttg ctggtgacgc ggtacagctt
     2101 gtgctgcgac cagcaggcga gagcgaatat tttcgcgcag atagatatcc tgatttggcg
     2161 ctaacagcgg tacggcggtg tcatcacgac ggcgaatcgt aatgaagttg tccataaagt
     2221 tctgccagct tttttgcaga ttgatacgcc attcgctgat ggaactggaa agctcttcac
     2281 cgtcggaatc catcggcgaa ccatcgctgt cattatcggc cagacgcagg ttatctacct
     2341 gatttgaaag ctgattaagc ttaaggatga tgccgtcata atccacctgc gatactgcag
     2401 aaaggctggc gatatcatcg gtaattgccc gacgaacggt aatcagactc gggtcattca
     2461 tatccgccag gctggcgtct gcacttttca gcaacgctgc agcggtcgtg acgtcctgat
     2521 cgctccacag cttccgtccg gcgagtttca ccagaaaatc ggcctgagcc agcagccagg
     2581 ttttagcatc gctgccggaa atggtggcga ccttttgttg gacttcatcc aactgttttg
     2641 ccagcgtttc ttgctgacga ttcgcctgct taagctgtgc agcttgttgc ttaataatgc
     2701 cttccagctc gactttttgg ctctcctggg ctttttgcaa tgccgtcagt tggttagcca
     2761 gggcatcgct ggtggcggtc tgattgacgg cctgttgttt accccagcca tacaaaccga
     2821 cgcccgccgc cagagcaata gcgatagcca ccgcgctgag aatcaatgcg gtattgttct
     2881 tactcttttt ttctgttgcg acaggttgtg acgtggtgtc cacggcctcc ctggtctctt
     2941 caaccacggc ggaggttttt tcttgttccg tcattatggc ttcctgttat gagagttatt
     3001 gtaatgcccg taaaagcgca tcgttgtcag cgttatcggc gaccttaatg tcttgccagc
     3061 ccagttcccg ggcgagtttc gccaaacgct cactgacgac caatagtcga cagtgtagta
     3121 accagtgctc acgataccat tgtgggatca gcgaccagag ttgctgcaac atttcaccgc
     3181 tggtaacaac gaccgtcgtc acctcgcggg attgccagcg catcgcttct tctgcaccat
     3241 cgtaatggat tgcgcatcgt tgataacatt cacaaaaagt gacctcagca ccgcgcgccg
     3301 tcagggtatc cccaattagc tcacgaccgc cattgccacg taatatcagc gcacgtttgc
     3361 ccgcaatatt ttgtaattca ggtaattgta gcaagacttc gctgatttcc cgatcctgcg
     3421 ggtagagaat cttctgtccg cttacggtat gtagtgccag tgcggtggtg cgtccaatgg
     3481 cgaaataatt aggtagtcgg ggccatttac gatcttgctg atgtagctgt gattgggcaa
     3541 aagcaaccgc gtgttgcgag agggcaaaca acagatcgct ctcccccagc gctgccagtt
     3601 gatcagcaag ttgcggtaat tgtcgacccg gagaaaactc aatcagcgga aaatgccagg
     3661 ccacctgccc cagtgtgcgc agacggctca ctaactcttc tccagcggga gacgggcggg
     3721 tgacaaggat actcatgccg gggcgtctcc gttatagact tcagcgagga tctcgcgcgc
     3781 gccgttattc agtagctctt ctgccagcga aatccccatt tgttcggcat cttgcggcgc
     3841 accgcggcgt tcaccgcgaa taatctgcga accgtccggc gcgccgacca gcgcacgcag
     3901 ccagatttcg ccatcaataa gctcggcgta gctaccaatt ggcacctgac atccaccttc
     3961 gagacgggta ttcatggcgc gttctgcggt aacgcgcagt gcagtttcgt ggtgattcag
     4021 cgcggcaagc agctcgcgag tgcgtgtatc atcaaggcgg cattcaatac cgactgcacc
     4081 ttgtcctacc gccggaagag aaatctcggg tggcaacgcg gcgcgaatgc gggactccag
     4141 acctaaacgt tttagtccgg ctacggcaag aatgatggca tcgtattcgc cgttatccag
     4201 tttgctcagg cgagtgccga cgttgccgcg cagggagcgg ataatcagat ccgggcggcg
     4261 ttcagccagt tggcactggc gacgtaaact ggacgtcccg acgatactgc ctgccggtaa
     4321 cgcatccaga ctgtcatagt tattggacac aaaggcatcg cgaggatctt cacgctcaca
     4381 aatagtgacc aggcccagac cttgcgggaa ttcaaccggc acatctttca ttgaatgtac
     4441 ggcgatatcg gcgcgatttt cgaggagcgc gacttccagc tcttttacaa ataagccttt
     4501 tccgccgact ttcgccagcg gcgtatcaag aatcacatcg ccgcgcgtca ccatcggtac
     4561 cagttcaacg accaggcccg gatggctcgc catcaacttg tctttgacat agtgtgcctg
     4621 ccagagtgca agtgggcttt ggcgtgtggc aattcttaaa acattgtcta acatgcttgt
     4681 taccgtcatt atcatccgtg gtccatccta acatccttgc cagagtgatg tcagtgttgt
     4741 ggtgaaacgt agacgcctgc gcaaaccgta aaatgaggtc tggcagtgga tcctgacagg
     4801 cgtttcacgc cgtcgtaata aggaatttac agagaataaa cggtgctaca cttgtatgta
     4861 gcgcatcttt ctttacggtc aatcagcaag gtgttaaatt gatcacgttt tagaccattt
     4921 tttcgtcgtg aaactaaaaa aaccaggcgc gaaaagtggt aacggttacc tttgacatac
     4981 gaaatatccc gaatgccgcg tgttaccgtt gatgttggcg gaatcacagt catgacgggt
     5041 agcaaatcag gcgatacgtc ttgtacctct atattgagac tctgaaacag agactggatg
     5101 ccataaatca attgcgtgtg gatcgcgcgc ttgctgctat ggggcctgca ttccaacagg
     5161 tctacagtct actgccgaca ttgttgcact atcaccatcc gctaatgccg ggttaccttg
     5221 atggtaacgt tcccaaaggc atttgccttt acacgcctga tgaaactcaa cgccactacc
     5281 tgaacgagct tgaactgtat cgtggaatgt cagtacagga cccgccgaaa ggtgagcttc
     5341 caattactgg tgtatacacc atgggcagca cctcgtccgt agggcaaagt tgttcctctg
     5401 acctggatat ctgggtctgt catcaatcct ggctcgatag cgaagagcgc cagttgctac
     5461 aacgtaaatg cagcctgctg gaaagctggg ccgcctcgct gggtgtggaa gttagcttct
     5521 tcctgattga tgaaaaccgt tttcgccaca acgagagtgg cagtctgggg ggcgaagatt
     5581 gtggctccac ccagcatata ctgctgcttg atgaatttta tcgtaccgcc gtgcgtctcg
     5641 ccggtaagcg tattctgtgg aatatggtgc cgtgcgacga agaagagcat tacgatgact
     5701 acgtaatgac gctctacgca caaggcgtgc tgacgccaaa tgaatggctg gatctcggcg
     5761 gcttaagctc gctttccgct gaagaatact tcggtgccag cctttggcaa ctctacaaga
     5821 gtatcgattc tccatacaaa gcggtgctga agacactgct gctggaagcc tattcctggg
     5881 aatacccgaa cccacgcctg ctggcgaaag atatcaaaca gcgtttgcac gacggcgaga
     5941 ttgtatcgtt tggcctcgat ccttactgca tgatgctgga gcgtgttact gaatacctga
     6001 cggcgattga agatttcacc cgtctggatt tagtacgtcg ctgcttctat ttaaaagtgt
     6061 gcgaaaagct cagccgtgaa cgcgcctgcg taggctggcg tcgcgcagtg ttgagccagt
     6121 tagtgagcga gtgggggtgg gacgaagctc gtctggcaat gctcgataac cgcgctaact
     6181 ggaagattga tcaggtgcgt gaggcgcaca acgagttgct cgacgcgatg atgcagagct
     6241 accgtaatct gatccgcttt gcgcgtcgca ataaccttag cgtctccgcc agtccgcagg
     6301 atatcggcgt gctgacgcgt aagctgtatg ccgcgtttga agcattacca ggtaaagtga
     6361 cgctggtaaa cccgcagatt tcacccgatc tctcggaacc gaatctgacc tttatttatg
     6421 tgccgccggg ccgggctaac cgttcaggtt ggtatctgta taaccgcgcg ccaaatattg
     6481 agtcgatcat cagccatcag ccgctggaat ataaccgtta cctgaataaa ctggtggcgt
     6541 gggcatggtt taacggcctg ctgacctcgc gcacccgctt gtatattaaa ggtaacggca
     6601 ttgtcgattt gcctaagttg caggagatgg tcgccgacgt gtcgcaccat ttcccgttgc
     6661 gcttacctgc accgacaccg aaggcgctct acagcccgtg tgagatccgc catctggcga
     6721 ttatcgttaa cctggaatat gacccgacag ctgcgttccg caatcaggtg gtgcattttg
     6781 atttccgtaa gctggatgtc ttcagctttg gcgagaatca aaattgcctg gtaggtagcg
     6841 ttgacctgct gtatcgcaac tcgtggaatg aagtgcgtac gctgcacttc aacggcgagc
     6901 aatcgatgat cgaagccctg aaaactattc tcggcaaaat gcatcaggac gccgcaccgc
     6961 cagatagcgt ggaagtcttc tgttatagcc agcatctgcg cggcttaatt cgtactcgcg
     7021 tgcagcaact ggtttctgag tgtattgaat tgcgtctttc cagcacccgc caggaaaccg
     7081 ggcgtttcaa ggcgctgcgc gtttctggtc aaacctgggg cttgttcttc gaacgcctga
     7141 atgtatcggt acagaaactg gagaacgcca tcgagtttta tggcgctatt tcgcataaca
     7201 aactgcacgg cctgtcagtg caggttgaaa ccaatcacgt caaattaccg gcggtggtgg
     7261 acggctttgc cagcgaaggg atcatccagt tctttttcga agaaacgcaa gatgagaatg
     7321 gctttaatat ctacattctc gacgaaagca atcgggttga ggtgtatcac cactgcgaag
     7381 gcagcaaaga ggagctggta cgtgacgtca gtcgcttcta ctcgtcatcg catgaccgtt
     7441 ttacctacgg ctcaagcttc atcaacttca acctgccgca gttctatcag attgtgaagg
     7501 ttgatggtcg tgaacaggtg attccgttcc gcacaaaatc tatcggtaac atgccgcctg
     7561 ccaatcagga tcacgatacg ccgctattac agcagtattt ttcgtgatga acgtgccgga
     7621 aagcgaggct tatccggcat gcaatcttag cggaaactga ctgtttcacc cgcctgctgc
     7681 gtcgccgcct gttccagcaa atcccggaag gtttcgccgc tgcgatcgca aatccactca
     7741 tcgcctttca gatcaaaatg gtagccgcct tgtttggttg ccagccatac ctggtgcagc
     7801 ggttcctggc ggttgataat gattttgctg ccattctcaa aggtaatggt cagcacgccg
     7861 ccgttgattt cgcagtcgat atcgctgtcg ccatcccagt cgtccagacg ctcttcaatg
     7921 gtcagccaca gttgatcagc caggcgatga aattcactgt cgttcattgt tgtatcctgt
     7981 ttttaagtga tggcggcagt atagcggcat ggggtcaggg cttcaaagtt tgcacctctg
     8041 cggctgcgtt acggcatgat tcatccgtca ccggaataat gatgtctctg tgtagcgaaa
     8101 gatttgtctc ttcattaggg cgcagttaca ccacgccttt ccctgtttct ggtaaacatt
     8161 atgatcaggt ttaccgagcg agcatcctca cgctgacgga actaaaaaag ccaacaaaca
     8221 aaccacattg cgatagtgca taaagccatc ctggcgtgag gtgttgatca cgaaactgcc
     8281 agcagaacat aaatcctcac gagtaagcgt tatactcgca gcatttcctc acttttcaga
     8341 cttcataaag agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag
     8401 aaagcgatga actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat
     8461 tacttactct cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg
     8521 cagataaaaa tgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc
     8581 cggataaaaa cgatcgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag
     8641 tttctgtacc cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca
     8701 acgattttat ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc
     8761 gtcgcctagc tgatcggcac ctgggggtag ggtttgacca actgctggtg gttgagccgc
     8821 cgtatgatcc tgaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg
     8881 cgcagtgcgg caacggcgca cgctgctttg cccgttttgt gcgtctgaaa gggctgacca
     8941 ataagcgcga tatccgcgtc agcaccgcca acgggcggat ggttcttacc gtcaccgatg
     9001 atgatctggt ccgcgtaaat atgggcgaac ccaacttcga accttctgcc gtgccgtttc
     9061 gcgctaacaa agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg
     9121 gcgtggtgtc gatgggaaat ccacattgcg tgattcaggt cgatgatgtc gatactgcgg
     9181 cggtagaaac actggggcct gttctggaaa gccacgagcg ttttccggag cgcgccaata
     9241 tcggttttat gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg
     9301 caggagaaac ccaggcctgc ggcagcggcg catgtgcggc ggttgcagta gggattcagc
     9361 aaggtttgct ggccgaagaa gtacgcgtgg aactcccagg cggtcgtctt gatatcgcct
     9421 ggaaaggtcc gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat
     9481 ttattcatct atgaagcaac caggggaaga actgcaggaa acactcacgg agcttgatga
     9541 ccgggtggtt gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc
     9601 agtagaagcg atacgtgtgc cgcatccggt acgcggtacc gtttcgttgg tcgagtggca
     9661 catggcccgc gcacgtaatc atattcatgt tctggaagag aacatggcgc tgttgatgga
     9721 acaggctatc gccaacgaag gcctgtttta tcgcttactc tacctgcagc gcagtctcac
     9781 cgccgccagc agtcttgatg atatgctgat gcgctttcac cgctgggcgc gtgatctcgg
     9841 cctggcaggt gcgagtctgc gcctgtttcc ggatcgctgg cgcttaggtg cgccgtcgaa
     9901 ccacactcat ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtctggg
     9961 gcaggagcag cattatctgg ggccgcttaa cggaccggag ctgctggtgg tactaccgga
    10021 agcgaaagcg gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttaggcgt
    10081 ggtgctgttc accagccgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact
    10141 tcatgaaatt gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc
    10201 gatttacaca ccgatgtaga acgctaccta cgttatctga gcgtggagcg gcagcttagt
    10261 ccgataactc tgcttaacta ccagcgacag cttgaggcga tcatcaattt tgccagcgaa
    10321 aacggcctgc aaaactggca gcaatgcgat gcagcgatgg tgcgcaattt tgctgtacgc
    10381 agtcgccgta aagggctggg cgcggcaagc ctcgcattac gcctctctgc gctacgtagc
    10441 ttttttgact ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggtgtttcg
    10501 gcaccgaaaa cgccacgtca tctgccgaaa aatatcgacg tcgatgatat aaatcgcctg
    10561 ttggatattg atatcaacga tcccctcgct gtgcgcgacc gtgcaatgct ggaagtgatg
    10621 tacggcgcgg gcctgcgtct ttccgagctg gtggggctgg acatcaaaca cctcgacctg
    10681 gagtccggcg aagtgtgggt gatggggaaa ggcagcaaag agcgccgcct gccgattggt
    10741 cgtaacgctt tgtcatggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa
    10801 gacgacgcgc tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa
    10861 cgctttgccg aatggggcat aaaacaaggg ctgaataatc acgttcaccc gcataaatta
    10921 cgtcactcgt tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag
    10981 ctgctgggtc atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac
    11041 cttgcctcgg tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt
    11101 accggccttt ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata
    11161 accgtccggt gattttgcgc accgagcagg aggcgcttac ctttgtgcaa aattaccatc
    11221 cggcgctgcg cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag
    11281 cggaacccga gatttatcac gacgtgacgc gctggcgttt tcgctcaata gaacaagcga
    11341 tgcttgacgc cggattgagc gcggaagaag ccagtgcagg cgcacacgcg gcaatgatca
    11401 actttgctaa atggcgcagc aggatcgacg tcccgcagca aacccacgac accttaaaac
    11461 agctggcgaa gaaatggccg ctggtggcga tcaccaacgg taacgctcag ccggaactgt
    11521 ttggtttggg ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcgaaac
    11581 cgttcagcga tatgtacttc ctggcggcgg aaaaactcaa cgtgccgatc ggcgagatct
    11641 tacatgtagg tgacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg
    11701 cctgttggat cagaccggaa aatggcgatc tgatgcaaac ctgggatagc cgtttactcc
    11761 cgcatctgga aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt
    11821 atatataccc agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc
    11881 ggcggtgcca atggacgttt cttacctgct cgacagcctt aatgacaaac agcgcgaagc
    11941 ggtggccgcg ccgcgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg
    12001 cgtactggtg catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat
    12061 tatggctgtg acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcgggcaact
    12121 gatgggcacc agccagggcg gcatgtgggt cggcaccttc cacgggctgg cgcaccgttt
    12181 gctgcgtgcg caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga
    12241 agaccagcta cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg
    12301 gccgccgcgc caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca
    12361 tcatattcaa agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta
    12421 tcaggaagcg tgcgatcgtg caggcctggt ggacttcgcc gagctgctgc tgcgcgctca
    12481 cgagttgtgg cttaacaagc cgcatatcct gcaacactac cgcgaacgtt ttaccaacat
    12541 cctggtggac gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc
    12601 gggcgacacc ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg
    12661 cggggcgcag gtggagaata ttcagcgttt cctcaatgat ttccccggtg ccgaaactat
    12721 ccgtctggag cagaactatc gttctaccag taacattctg agcgccgcga acgccctgat
    12781 tgaaaacaac aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc
    12841 tatttccctc tattgcgctt ttaacgaact cgatgaagcg cgttttgtcg ttaaccgcat
    12901 caaaacctgg caggacaacg gtggggcgct cgccgagtgc gccattctct accgcagcaa
    12961 cgcccagtcg cgggtgctcg aagaggcgtt attgcaggcc agtatgccgt accgtattta
    13021 cggcgggatg cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct
    13081 gattgccaac cgcaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg
    13141 tattggtgac cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact
    13201 ctggcaggca tgtcgtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc
    13261 cttacagcgg tttatggaac tgatcgacgc cttagcgcag gaaactgccg atatgccgct
    13321 gcatgtacag actgaccggg taattaaaga ctccggcctg cgtaccatgt acgagcagga
    13381 gaagggcgaa aaaggtcaga cgcgtatcga aaacttagag gaactagtga cggcaacgcg
    13441 ccaattcagc tacaacgaag aagacgaaga tttaatgccg ctgcaggcgt tcctctccca
    13501 tgcggcgctg gaagcgggcg aggggcaggc ggatacctgg caggatgcag tgcagttgat
    13561 gacgctgcac tcggcgaaag gcctggagtt cccgcaggtg tttatcgtcg gtatggaaga
    13621 gggcatgttc ccaagccaga tgtcgctgga tgaaggcgga cgtctggaag aagaacgccg
    13681 tctggcctac gttggtgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac
    13741 tcgccgtctg tatggcaaag aggtttacca tcgcccgtcg cgctttatcg gtgagttgcc
    13801 ggaagagtgt gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca
    13861 gcgtatgggt acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgtgtacg
    13921 ccacgctaag tttggtgaag gcactatcgt caatatggaa ggcagcggcg agcatagccg
    13981 tttgcaggtg gcattccagg gccagggtat taaatggctg gtggcggcat acgcccggct
    14041 ggagacggta taacgttgcg ggatgcggtg cgccacaccg catccggcat cagcacctta
    14101 cttatcacat ttcttcacat caatattcca gtcagcacca gacgcaacct gatgtttctg
    14161 cgcgaaatta tccatattca gcgccacgga aacattcaat aagctgttca gatacaccat
    14221 cggctgtcct tccggcacat cgccgaagct tgcgacatac ggcattttcc ctgcgtattt
    14281 ttgccgggaa ccttcggaaa tggtcacaca caacgtgtcg ttgagtttga tccccgcctg
    14341 attcagcaac tcgtcgctga tattgctcca gacgttgccg tactgaatat cgagaatcgg
    14401 gatattgccc ttcacttcac cttttgttgc ttttgctttc tgataagaaa gttcgacaac
    14461 ctttgcagga agctcaggcc ccacctgctc gaacgtgatc gccccggaag ccagacgtgc
    14521 accggtgtag gcatacacgt cacgaccgtg gaaggtatat gacttttcag aacctttcag
    14581 gcggttagct ttttcgtcga tttcccggac gctctcaata cccagcgatt ccgccaccaa
    14641 agtgagcgta ccgttatccg gtgaaacgaa gtattgaccg tttttggttt tcagcactat
    14701 cgatttacgg tcagtgccta cgcccggatc gaccacactg acaaataccg aaccttgcgg
    14761 ccagtaactg gcggtctgat acaggcggta agcgccttcc cagatgttat acggtggaat
    14821 ttcgtgtgtt aaatcaaata ttttaagatt atggtcaaca ccgaatgcga cccctttcat
    14881 cgccgagacg gcaccatctt tcaggctaaa atcagtttgt aaaatcaaag cgttatccgc
    14941 ataagcggat gatgatccca gacacgttaa taaaaataac gttgcgagcg tttttttcat
    15001 ttttgtttcc cttcatttat ctgatgattt tccagctgtg tagccagccc cagtttgtct
    15061 ggcctcctcg agtgtacaat gcttaacctt tccgctcgac ggaaatcatg gtgacaaaag
    15121 gatagcgttg ccacgggatt gccccgcctt tcatatacat atgtgaaatc gtgccatcaa
    15181 gataaagcag ctgctcaacg ttcagtttcg ctttggcata acaggcaaaa tcataaaaat
    15241 ttgtcgcctg ctggctcaac aaaaacacgg cattcccatg tttattaatt ccaacaccgt
    15301 tacgaatttt acgtgaggcg acgttgggat gaatacgcgg attaattaca ctgttttcca
    15361 tcaacattgg ccctgactgc accgcaaact gaatctcttt actggttttg aaggcatcca
    15421 gacgaacgat gccgacttta tctcccgcga cataaaacac gccgccagga cggataaaga
    15481 aattcccttc acctgaagcg agatttaacg ccaccttctg ctgaccgttt tcgatgtaca
    15541 aaccgagcgg cgcatagctt tcatcataga tgccgccgtt catcgccatc tgcacctgac
    15601 cctgactatt catatccgcc agcagagcat gtaaagttcc ccacgcttcg ccattggctt
    15661 tttgccagta cattttcacc cgctctgttt gaggattaac ggtatacgcc tgtacggtca
    15721 gcgttggatc tgagagtgcg caatcatcag cggcaacagc aaacaaggga agtaaggtga
    15781 gggcgagaaa aatccgtttg agattcaagg tgatcattcc tttaccaatg agtagccgat
    15841 gcgccattat aggtcctgga tgtgggattt ttttatcctg ttagcgacct tgacgagtac
    15901 caaaaagcgc gaagttcaac tattgttctg tggtgttctg ttgcgtgttg acggcaaaat
    15961 tttgctggcg taacatgcgc gcacgatcac tctaagagga cattcgcctt ggacacaccc
    16021 agtagatact ggctcactat cctgtcatcc aggatcaact cctaaggcta tccctttttg
    16081 ctgatagcct tagcggttgt cagcgacctc aatttttccc gtcgcgctga gtcaggctgt
    16141 ttaatggtct gaaacccaat ttgtttctgt gtgcccaccg aactgtccga tattttaagc
    16201 attgggagtc ccggtcatgc tgagcgcatt tcaactggaa aataaccgac tgacccggct
    16261 ggaagtcgaa gagtcacaac cccttgtaaa tgcagtatgg attgatcttg tcgaaccgga
    16321 cgacgacgag cgactgcgcg tacaatctga acttggccag agcctggcaa cccgcccgga
    16381 actggaagac atcgaagcat cggcacgttt ctttgaagac gatgacggcc tgcatattca
    16441 ctccttcttc ttctttgaag atgcggaaga tcacgccggt aactccactg tggcatttac
    16501 catccgtgat ggtcgtctgt ttactctgcg tgagcgtgaa ctgcccgctt ttcgtctgta
    16561 tcgtatgcgt gcccgtagcc agtcgatggt agacggtaac gcctacgagt tgctgctgga
    16621 tctgttcgaa accaaaatcg aacagttggc agatgaaatt gaaaatatct atagcgacct
    16681 ggagcaattg agccgggtga ttatggaagg gcatcagggc gatgagtacg acgaggcgct
    16741 ctccactctg gcggaactgg aagatatcgg ctggaaagtg cgcctgtgtc tgatggatac
    16801 ccagcgcgcg ctcaacttcc tggtacgtaa agcacgctta ccgggtgggc aactggagca
    16861 ggcgcgtgaa atcctgcgag atatcgaatc cctgctgccg cataacgaat ccctgttcca
    16921 gaaggtgaac ttcctgatgc aggcggcaat gggttttatc aacatcgagc agaaccgcat
    16981 catcaaaatt ttctcggtgg tatccgtcgt gttcctgccg ccaacgctgg ttgcttccag
    17041 ctacgggatg aactttgagt ttatgccaga actgaagtgg agtttcggct acccaggcgc
    17101 gattatcttt atgatcctcg cgggcctggc accgtatctg tactttaagc gcaagaactg
    17161 gctgtaacta tacgatgtcg ctgtgagtaa gttagtgtgc tgacagcgac attttagcag
    17221 agggaatcgc agataacata attgttgtga ataatatcca tgccacgttg agttggacgc
    17281 ttaatgactc acatctcacc ccttcaattg ctgcagagat aatagaaaaa agtgtcaaac
    17341 ccaatgaaat caatacaaag tagattgcct tacatccagt atctcttatt
//
LOCUS       DAZFBR010000099        17386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFBR010000099 DAZFBR010000000
VERSION     DAZFBR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.099"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(177..1205)
                     /gene="epmB"
                     /locus_tag="ACHJZ2_003645"
     CDS             complement(177..1205)
                     /gene="epmB"
                     /locus_tag="ACHJZ2_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO4569681.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1247..1813
                     /gene="efp"
                     /locus_tag="ACHJZ2_003646"
     CDS             1247..1813
                     /gene="efp"
                     /locus_tag="ACHJZ2_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO4569682.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1865..1990
                     /gene="ecnA"
                     /locus_tag="ACHJZ2_003647"
     CDS             1865..1990
                     /gene="ecnA"
                     /locus_tag="ACHJZ2_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO4569683.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2101..2247
                     /gene="ecnB"
                     /locus_tag="ACHJZ2_003648"
     CDS             2101..2247
                     /gene="ecnB"
                     /locus_tag="ACHJZ2_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO4569684.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2423..2740
                     /gene="sugE"
                     /locus_tag="ACHJZ2_003649"
     CDS             2423..2740
                     /gene="sugE"
                     /locus_tag="ACHJZ2_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO4569685.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2737..3270)
                     /gene="blc"
                     /locus_tag="ACHJZ2_003650"
     CDS             complement(2737..3270)
                     /gene="blc"
                     /locus_tag="ACHJZ2_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO4569686.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3358..4491)
                     /locus_tag="ACHJZ2_003651"
     CDS             complement(3358..4491)
                     /locus_tag="ACHJZ2_003651"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4569687.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(4554..4913)
                     /gene="frdD"
                     /locus_tag="ACHJZ2_003652"
     CDS             complement(4554..4913)
                     /gene="frdD"
                     /locus_tag="ACHJZ2_003652"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO4569688.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4924..5319)
                     /gene="frdC"
                     /locus_tag="ACHJZ2_003653"
     CDS             complement(4924..5319)
                     /gene="frdC"
                     /locus_tag="ACHJZ2_003653"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO4569689.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5330..6064)
                     /gene="frdB"
                     /locus_tag="ACHJZ2_003654"
     CDS             complement(5330..6064)
                     /gene="frdB"
                     /locus_tag="ACHJZ2_003654"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO4569690.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6057..7865)
                     /gene="frdA"
                     /locus_tag="ACHJZ2_003655"
     CDS             complement(6057..7865)
                     /gene="frdA"
                     /locus_tag="ACHJZ2_003655"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO4569691.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8190..9167
                     /gene="epmA"
                     /locus_tag="ACHJZ2_003656"
     CDS             8190..9167
                     /gene="epmA"
                     /locus_tag="ACHJZ2_003656"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO4569692.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9386..10888
                     /gene="yjeM"
                     /locus_tag="ACHJZ2_003657"
     CDS             9386..10888
                     /gene="yjeM"
                     /locus_tag="ACHJZ2_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO4569693.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11039..14362)
                     /gene="mscM"
                     /locus_tag="ACHJZ2_003658"
     CDS             complement(11039..14362)
                     /gene="mscM"
                     /locus_tag="ACHJZ2_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO4569694.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14384..15352)
                     /gene="asd"
                     /locus_tag="ACHJZ2_003659"
     CDS             complement(14384..15352)
                     /gene="asd"
                     /locus_tag="ACHJZ2_003659"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO4569695.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15449..16501)
                     /gene="rsgA"
                     /locus_tag="ACHJZ2_003660"
     CDS             complement(15449..16501)
                     /gene="rsgA"
                     /locus_tag="ACHJZ2_003660"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO4569696.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16596..17141
                     /gene="orn"
                     /locus_tag="ACHJZ2_003661"
     CDS             16596..17141
                     /gene="orn"
                     /locus_tag="ACHJZ2_003661"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO4569697.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         4019 a         4485 c         4568 g         4314 t
ORIGIN      
        1 aaatgggctt ggtagtaata gttgttaaaa taacataaat agccgtactc actctatata
       61 aacagaaaag aaaggttaat tattggtgtt ggttatataa agagtaacaa cagcaatgta
      121 tatgaataat taaaggttat gtaacgggca aagattaaac ttccctgttt aatcagttac
      181 tgctggcgta gctggagatc cagcggcgtt ttgctgggtt cgccgccaat ttctcgcgcc
      241 agtttcggca ccagatatcc cgacaccagt gtcagcaact cacgcataat ctgccgtgct
      301 tcgtcatcac tcaccataaa atgcgccgcg ccctgtactt tatcgagcac atgcaggtaa
      361 tacggcatca cgcctgcatc gaacaacgca ttactcaggt ttgccagcgt ttgtgcgtta
      421 tcgttcacac cacgtaacag aacgctctgg ttcagcaaag taacgcccgc ccggcgcaac
      481 ttagccatcg cctgacggaa tgtttcatct atctcattgg catggttgat gtgattcacc
      541 agcaagattt gcagcgtaga atgtgaaaag cgttcaacca gcgcatctgt gatgcgtgcc
      601 gggatcacaa tcggcagacg actgtgaatc cgcagacgtt tgatatgcgg gatggcttcc
      661 agttgtgtga gcaaccagtc cagctcgtga tctttcgcca tcagcggatc gccgccggag
      721 aaaatcatct cgtccagttc cggatgcgca gcaacatact caagtgcagt ttgccagtta
      781 cgcttgttgc cctgattttc ggcatagggg aagtggcgac ggaagcaata gcggcaattt
      841 accgcgcagc cgcctttgac cagcaaaagc gcccggttgt ggtatttatg caacaaacca
      901 ggcactacgc tgtgctgttc ttccagcggg tcggtggaga atccgggcgc gacgacaaac
      961 tcatcttgcg aggtaagtac ctgacgcaaa agcggatcgt ccggattgcc tttctccatg
     1021 cgatcgataa atgagcgggg cacacgcagg gcaaacagct ttttggcgtt gcgtccggcc
     1081 aacagttttt cgtccgcgtc tatattcaaa agacgcagaa gttcatcagg atcggtcaca
     1141 acatcggcaa gttgcgttaa ccaatcttct ctggatgggg tatttagggt tacaatatgc
     1201 gccattttgt ggcttagcta ccaattaaca aatttcagag ggccttatgg caacgtacta
     1261 tagcaacgat tttcgtgctg gtcttaaaat catgttagac ggcgaacctt acgcggttga
     1321 agcgagtgaa ttcgtaaaac cgggtaaagg ccaggcattt gctcgcgtta aactgcgtcg
     1381 tctgctgacc ggtactcgcg tagaaaaaac cttcaaatct actgattccg ctgaaggcgc
     1441 tgatgttgtc gatatgaacc tgacttacct gtacaacgac ggtgagttct ggcacttcat
     1501 gaacaacgaa actttcgagc agctgtctgc tgacgcaaaa gcaattggcg acaacgctaa
     1561 atggctgctg gatcaggcag agtgtatcgt aactctgtgg aatggtcagc cgatctccgt
     1621 tactccgccg aacttcgttg aactggaaat cgttgatacc gatccgggcc tgaaaggtga
     1681 taccgcaggt actggcggca aaccggctac cctgtctact ggcgctgtgg ttaaagttcc
     1741 gctgttcgta caaatcggcg aagtcatcaa agtggatacc cgctctggtg aatacgtctc
     1801 tcgcgtgaag taatgcggtt gtggtgcggc ctgcaggctg caccatcact tattcaggtc
     1861 agagatgatg aaacgcctta tcgttcttgt tttgcttgcc agcacgctgc tcacgggctg
     1921 taacaccgct cgcggtttcg gcgaagacat caaacatctc ggcaactcca tctcccgcgc
     1981 tgccagctaa tttttcttct cttccgaaaa atcatcagat ttccatcatt tttggcgatg
     2041 ttgtctatta ttaatttgct ataggcaaac ataaataaca ttacctaaaa ggaagacgtt
     2101 atggtgaaga agacaattgc agcgatcttt tctgttctgg tgctttcaac agtattaact
     2161 gcctgcaaca ccacgcgtgg cgttggtgaa gacatttctg atggcggtaa cgcgatttct
     2221 ggtgcagcaa cgaaagcgca gcaataagca ataacggtac gacagctgtg tcgtgccgtt
     2281 tgttttttct gcgatagtca caaaggtaat agttgaaatt cccccgccac ctggcaaaat
     2341 atccgttcaa ccatcagctt tgcaggacga cctgcaaacg cgtcttttca ccggggacgg
     2401 ccccaattct ccggagcctg atatgtcctg gattatctta gttattgctg gtctgctgga
     2461 agtggtatgg gccgtaggcc tgaaatatac ccacggcttt agtcgtttga cgccgagtgt
     2521 tattaccgtg acggcgatga ttgtcagtat ggcgctactt gcctgggcga tgaaatcgtt
     2581 accagtaggg acggcttatg ccgtgtggac gggtattggc gcagtcggcg cggccatcac
     2641 cggcattgtg ctgctcggtg agtccgctaa cccgatgcgt ctggcgagtc tggcgttaat
     2701 cgtattgggg attattggtc tgaaactcag cactcactaa ctaccgggct gctgtaccca
     2761 aataaactta ctgacatcaa acccttcccg ggtcgcgact gccagcatct cctgtttcac
     2821 ttcgtcagaa atggttggcg tgcgggagag tatccacagg tagtcgcggt ccggtccgca
     2881 aaccagcgca tggcggtatt cccgatcgag tgcaataacg ttataaccgc catagaaagg
     2941 accaaagaat gacactttca gcgcagcgcg agttggtgcg ccggtaaagt acgctttccc
     3001 ttcactctgc tgccacattc ctctgtcagg gttatagcct ttattaatga cattcaggcc
     3061 gccgtcatca cgcaggctgt atgttgcggt gactttttcc agtccacgtt caaagcggtg
     3121 atcaaaacgg gcaatctcat accaggtacc gagatagcgt ttagcgtcga aattattcac
     3181 tacggtcacg ccacgcggcg gcgtaggaga actgcaggca acgaccagaa atgcagctgt
     3241 cgctgcggca acgagaggga gcaggcgcat aaatgtttcc ttactggttt tttctaagtg
     3301 tagatgacag caaggaaaag cgcagaaaag gtccgaaaat tcggacccga tgaaatttta
     3361 ctgtagagcg ttgagaatct gccaggcggc ggcgactctc gctggattgg gatagttttt
     3421 gttagccagc atcacgatac ccagctcttt ttctggaata aacgcgacat agctaccaaa
     3481 tccgccggtc gcccccgttt tatgcaccca tgatgctcgt actgcaggag ttggaggcgt
     3541 aatcgctttt acggggcgtg ctgccagtgc aattttattg tcactgccgt taacgatgat
     3601 gtcaggattt accggccagt ccagcatttc ccagcccaga ccctgataca tatcgccggt
     3661 ttgccagtag cgagattgtg ccagttgtat cccttgctga agtgttttgt cgttgatatc
     3721 acggggattc atattgcttc gtacccagca ggccatatct tcaatggtcg acttcacacc
     3781 ataagtttca gcatctaacg cccctggcga aacatgaact gccttgcctt cgcgatatcc
     3841 ccaggcgtaa ttcttttctt ctgcgggcgg tacattaatc cacgtatggt tgagtttgag
     3901 tggctggaag acacgagttt gcatcgcctg ctcaaaactc aaaccagacg gcttcacagc
     3961 cagtgcgccg aacaaaccga tactggagtt ggcatacaga cgttgtgttc ctggagccca
     4021 tgcaggctgc cagttttgat agaagcgcag caagtcgctt gaggatttca cttcatccgg
     4081 cacctgcaat ggcaggccgc cagcagtgta ggttgcgaga tgtaatagtg tgatcccatt
     4141 ccactgttta gcggtaagtt caggccagta ttttgttgcg ggatcgctta acttgatttc
     4201 ccctcgagca atagcgtcgc caccaagcac gcccgtaaat gttttgctga ccgaacctaa
     4261 ctcaaacaac gtttgctgtg tgacgggctg ctttttggcg atgtccgcat acccccaggt
     4321 aaagtaataa ggtttaccct gataaattac cgccaccgcc ataccgggga tcttttgttg
     4381 ctctataagc ggggtaattg tgcgatgcac aatatcgttg atttgttgag gggcagcaaa
     4441 tgtggagcaa gaggcggtaa ttaataaggc gcagagcgtc gttttgaaca tagggtctgg
     4501 tttccataca aaacggcccg ccataggcgg gccggattta cattggcgat gcgttagatt
     4561 gtaacgacac caatcagcgt gacaactgtc aggatagcag ccagaccgta gaaaacccat
     4621 ttgcccgcag gtacgtggat tttcagatcg tgcatcgcgt ggtgcatacg gtgtaaacca
     4681 caccacagcg gcagaacgat catcaggaac aggaatacgc gaccaatgaa gctctgcgcg
     4741 aacgccagaa cgcgctcgta gctcagtgca tcacccggaa acagccccag cggcagcaga
     4801 atacccacca gcaggatcat caccggcgca ataattgcgc tccacatacc accggccccg
     4861 aagaggcccc agaataccgg ttcgtcagaa cgctttggat ttggattaat catctcaggc
     4921 tccttaccag tacagggcaa caaacaggat tacgatggtg gcaaccacag ttaccgccca
     4981 gagacttttg ataattggct ctggtcccat tttttcgtct tttacaatga tattggccgc
     5041 tttcggtgcc agttcaaacc aggttttggt gtgcagcaga gctgccgcca gagtgatcag
     5101 gttaatgatc acaataaccg ggttttgtaa aaagtcgacg aatcccgccc aggcttccgg
     5161 gccatttttc agggcaaaca gcccgaaaat cagttcaatg ctgaaccaca cagccggaac
     5221 cgccgtgcct tcgcgcagca tgtaaaagcg ataaaacggc aattttttcc accaggtgga
     5281 cgtcattggc cgtacatacg gtttacgttt agtcgtcatg ttgcactcct tagcgtggtt
     5341 tcagggtcgc gataagaaag tctttcgaac tttctacttt gccctgttga atggccgcag
     5401 ccggatcgac gtgtttcggg cagacttcgg agcagtagcc cacgaaagta cagctccata
     5461 cgccgttctg gctgttcaac tgcgccatac gctccttctt accgtggtcg cggctatctt
     5521 cgttataacg atgcgccagc gtaatggcag ccggaccgat gaactctggg ttcaggccaa
     5581 actgcgggca cgcggcgtag cacagaccac agttgatgca accggagaac tggtgatact
     5641 tcgccatctg cgccggggtc tggatgttag taccctgatc cgcggtgcgg gagttgccga
     5701 tgatgtacgg tttgatcgct tccagacttt cgatgaagtg ggtcatatcg accaccagat
     5761 cgcgttcaat cgggaagtta gctaacgctt caaccttcat accgtcggtg taatcacgca
     5821 ggaaggtttt acatgccagt tttggcacgt tgttaaccat catgccgcag gaaccacaaa
     5881 tcgccatacg gcaggaccag cggtagctca ggtccggtgc caggttgtct ttgatgtagc
     5941 ccagcgcatc cagtaatgag gtagttgcgt cataaggcac ttcatagaat gcgctatgcg
     6001 gtgcggtatc gacttccggg ttatagcgca ccacctcaat tttcaggttt ttcatctcag
     6061 ccattcgcct tctccttctt attggctgct tccgccttat cggctgcatc cgcttcgcca
     6121 ccgtaaacgc gtttagctgg cggtagcgta gtaatcttca cgtcgctgta ctccaggcga
     6181 gtcgtgccat cagcatcgcg gaaggcgagg gtgtgtttga ggaagttgac gtcgtcacgc
     6241 ttggtgcaac cttcgtccag acgctggtgt gcgccgcggg actctttacg tgccattgcg
     6301 gagtgcgcca tacattcagc aacgttcaga ccgtggccca gttcaatggt gtagagcagg
     6361 tcggtgttga acacgctgga agtgtcggtg atgcgcacgc gcttgaagcg ttcctgcagc
     6421 tctgccagct tgtcgatggt tttctgcatc agttccggcg tacggtagat accgcaacct
     6481 tcttccatag ccaggcccat ttcgtcgcgg atcttcgccc agttttcgcc gccatcctgg
     6541 ttaaccagat ctttcagacg ttgttcaacg ccagctgcct gcgcttcaat tgccgcttcg
     6601 ttgccattac cggcagttgc tgcacgctct gtcgcttgtt caccggccag acggccgaag
     6661 accaccagtt ccgccaggga gttggagccc agacggtttg caccgtgcag accaacagag
     6721 gaacattcac ccacggcgaa cagaccttta atgcgggttt cacagttctg atcggtttcg
     6781 ataccgccca tggtgtagtg tgcggtcgga cgtaccggaa tcggttcttt aaccggatcg
     6841 acgccaacgt acgctttcgc cagttcgcag atgaacggca gacgttcatg cagttttttc
     6901 tcgccgaggt gacgcaggtc gagataaacc acatcaccac gcggcgtgga gatggtgttg
     6961 cctttacgcc attcgtgcca gaaggcctga gaaactttgt cgcgtggacc cagttccata
     7021 tatttgtttt tcggctcgcc cagcggagtt tccgggccca tgccgtaatc ttgcagataa
     7081 cggtagccat ttttgttgac cagaataccg ccttcaccgc ggcaaccttc ggtcatcagg
     7141 ataccggaac ctggcagacc ggttggatga tactgaacga attccatgtc acgcagcgga
     7201 acgccgtggc ttagcgccat acccataccg tcaccggtaa cgatgccgcc gttggtgttg
     7261 taacgataaa cgcgacccgc accgccagta gccataacga ccgcgttagc acggatctgc
     7321 accagcgtgc cttccatcat gttcattgct accaggccgc gaacatgacc atcatcaacc
     7381 agaatatcca gcacgaaatg ttcgtcaaaa cgctggatct gcgggaattg cagagaggtc
     7441 tggaacagcg tgtgcagcat atggaagccg gtcttatcgg cggcgaacca ggtacgctcg
     7501 attttcatgc cgccgaagcg acgtacgttg acgctaccat ccgggcgacg gctccatggg
     7561 cacccccaca gttccagttg ggtcatttcg gttgggcagt ggtggacgaa ataatccacg
     7621 acatcctgct cacacaacca gtcgccaccc gctactgtat cgtgaaagtg atattcgaag
     7681 ctgtcatgat cctgcgcgac agcggcggag cccccttctg cagcaacggt atggctacgc
     7741 atcgggtata cttttgagat tagtgcgatt tttgcatttg gatttgcctg cgcggcagca
     7801 attgcagcac gtaatcccgc gccaccggcg cctacaatgg caagatcggc ttgaaaggtt
     7861 tgcacgacat tcctccagat tgtttttatc ccacagcccc gtacttcagg gtaagtgcct
     7921 gaaagttacg gtctgcgaac gctattccac tgctccttta taggtacaac agtatagtct
     7981 gatggataag tctgaaattt gacgagatcg atttttttag tgcgcaagag ggctaaatta
     8041 tcactgaaga tgattaattt aattactaaa ccatcagatc gtgctttttt tagtcactgc
     8101 ccaccgttgt tttattcctg cgtaaaattt gtctcgccgt cgcgttgcga gtagacttcg
     8161 tgcccttgtc aaaaactgga gatttaacta tgagcgaaac ggcatcctgg cagccgagcg
     8221 catccattcc taacttatta aaacgcgcgg cgattatggc ggagatccgt cgtttctttg
     8281 ccgatcgtgg agtgctggag gtggagacgc cttgtatgag ccaggcgacg gtaaccgata
     8341 ttcatttggt cccgtttgag acacgtttcg ttggccccgg gcattcgcag gggatgaatc
     8401 tctggttaat gaccagcccg gaataccata tgaaacgcct gctggttgcc ggttgcgggc
     8461 cggtattcca gctgtgccgc agtttccgta atgaagagat ggggcgttat cacaaccctg
     8521 agttcactat gcttgagtgg tatcgaccgc actatgatat gtaccggttg atgaacgagg
     8581 tggacgatct cttacaacag gtgctggact gcccggcagc agaaagcctt tcttatcaac
     8641 aagctttctt gcgttatctg gaaattgacc cgctctctgc cgacaaaacg caactgcggg
     8701 aagtggcagc gaaactggat ttgagcaatg ttgctgatac cgaagaagac cgcgacacgt
     8761 tgctacaatt gctgtttacc tttggcgtag aaccaaatat tggtaaagaa aaaccgacct
     8821 ttgtgtacca ctttccagcc agccaggcat cactggcgca aatcagtacc gaagatcatc
     8881 gggtcgctga acgctttgag gtttattata aaggtattga gctggcgaat ggtttccatg
     8941 aattgacgga tgcccgtgag cagcaacaac gctttgaaca agataaccgt aagcgcgcgg
     9001 cgcgcggttt gccgcagcac cccattgatc agaatctgat tgaagccttg aaagtcggta
     9061 tgcctgactg ttccggcgtg gcattaggtg ttgatcgtct ggtgatgttg gcgctgggcg
     9121 cggagacact ggctgaagtc atcgccttta gcgttgaccg ggcataattc tgaaaaatta
     9181 ctgaattaac agcgaagaat ggcgtgataa gcgccattct gtagcatatg ttttcatttt
     9241 tccgctaaag gccacaattt aagactggat atttgatatc atccaggtat caattctgta
     9301 ttggttttta cgctgcccgc tcaatgttgg gcagtgcagt tcagtgggcg cgttttatac
     9361 acgcgctgaa atgaaggatg gtttcatgcc tcacacgata aaaaagatga gtctgatagg
     9421 actcatattg atgatcttta cttccgtatt tggatttgcc aatagcccat cggcttatta
     9481 cttaatgggt tatagtgcga ttccctttta tatattttct gcattgttat tctttattcc
     9541 attcgcctta atgatggctg aaatgggagc tgcttatcgc aaagaagaag gcggtatcta
     9601 ttcctggatg aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt
     9661 ttcctcttat atcatctgga tggtgagcac ctccgcgaaa gtttgggtac cgttctcaac
     9721 attcctctat ggtagcgaca tgacccagca ctggcgtatt gccggactgg agcctacgca
     9781 ggtggttggt ctgctggcag tggcatggat gattctggtc accgtcgttg cttcaaaggg
     9841 gattaataaa attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt
     9901 agtattgctg ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga
     9961 tattaatttc cttgcatcac cgaacccagg ttatcagtcc ggtctggcaa tgctatcgtt
    10021 tgtggtattt gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa
    10081 aacggaaaat ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc
    10141 aatcggttat tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt
    10201 aagtaatggt tccgttaacc tcggcaatat tacctatgtg ctgatgaaga gcctcggggt
    10261 gacgctgggt aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt
    10321 tgcgcgtatt accggacttt cgatgttcct cgcttatacc ggtgcgttct ttacgctttg
    10381 ctattcaccg ctgaaagcca tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat
    10441 gacgcgcctg aatgcgatgg ggatgccgtc tatcgctatg tggatgcagt gcgggttggt
    10501 tactgtcttc atcctgctgg tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa
    10561 gctgacgctg atggcgaacg tgtctatgac gcttccttac ctgttcctcg cgctggcttt
    10621 cccattcttt aaagcacgtc aggatctcga cagaccattt gtgattttca aaacgcgtat
    10681 gtcggcaatg attgcgacgg tggttgtcgt tctggtggtg acatttgcga acgtcttcac
    10741 cattattcag cctgtggttg aagccggaga ctgggacagc acattgtgga tgattggcgg
    10801 ccctgtcttc ttctcgctgt tagcgatggc gatttaccag aactattgca gtcgcatggc
    10861 gaataaacct gagttagctc tcgactgatc atatgaaatc ctgcaactca tcgtctctcc
    10921 ggcgatgagt tgtcaggaag taatgcgtat ggatgatact ctccagggcc ggtgataact
    10981 gaagatgatg ttaagctgct aaatgagcca cgttgacatc atttggcagc ttcatgcatt
    11041 acaaacttcc cgcctgacga cctttgcccg cagacgtcag cgttctcccc gtttgtttac
    11101 cgttgaggct ttccagacgc atctggaagg gcgggaatgg catatcgata ccgtgggcat
    11161 ggaagccagc cagaatcagc tggtggatct catggcgtag cggcatacgg tgacccatct
    11221 cagcggcgta aatacgcagc tcgaaaatct gaatcccctg ttgcagatcc accaggaaga
    11281 cttccggtgc cgggttgtcg atcaccagcg agcagcgacg cgctgcggtg agcaggattt
    11341 ccgtcacttc ttcactgttg gcatcggcag gggccggaat cgtcaacacc acgcgcgtga
    11401 ccgagtcaga gagcgaccag ttgataaact gctcggtaat aaacgccttg ttcggcacga
    11461 ttatctcttt acggtcccag tcgctgatgg tggttgcgcg ggtattaatt ttcgtcacgc
    11521 taccagtaag atcgcgaatt gtcaccgtat cgccaatgcg aatcggtttt tcgaacagga
    11581 tgatcaggcc agagataaag ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac
    11641 cgagcgcggc gaccagccac tgcaatttcg accactcaat accaatcatc gagaagccga
    11701 ccagcccgcc aatcagcatc agcagatatt tggtgatggt ggtgatggcg tagccggtac
    11761 ccggcgttaa atccaggtgc tgcaaaatcg ccagttccag cagcgcgggc agattgcgca
    11821 ccagctgcgt ggtgatgata aacaccagaa tggcaatcag caccgcaccg agggtaatcg
    11881 gctccagact ttctacgccc tgtaccgtgg aggtgacatc ccacagcgaa atattttcga
    11941 ggaagccgaa agcggaatgg atttctgacc acagcacaat gaccgaaagc agggcgatca
    12001 acatcaaaat tgagcgcact agccgcaagg actgcgcact gatggcatcg agatcgactt
    12061 cgctttcatc gacttcaatt gccccttccg ggctactgtg atgatgtgct tcctcttcgc
    12121 cacgcgcacg ttgcgctaac atctctgccc ggcgatgctt cgcccggtca aacgccagcc
    12181 tgcgtcgttg gatcagcatc cagcggcgga taacgtgata aaccaccagt agcaggaacc
    12241 agatggcaac cgaggtttca agcctcgcta acagcgcctg tgccgttgcc agataaccca
    12301 ccgccgacgc cagaatggca accagtggcg cgccaatcat catgttccac aacatatggt
    12361 tggtaatatt gtcgccgctg ccttctttgt tgagatacag cgggatcccg gcctttttca
    12421 ggctgagggt gaccaccgcc agcgcaccgc aaatgaggat aaagcaaagc cgtcccagcg
    12481 aaccggaaaa ttcgcggtcg tcgaggttat cgaacatcat cagcgccata atcagtggca
    12541 caataagccc gatgctcatc aggtagtagc gcatcccacg agaaacacgt tcgcgcggcc
    12601 agccaaaatg agcgataaac aagccgttcg ggcgggcaaa ggtagcgcaa atcatcacta
    12661 cccacagcag cggcacggtg gctgtgacgc catcaccaat cgcgaccgcc agcggataag
    12721 gccacgcctc gcgcaagccg taacccagcg tcatccacag caccggcagc ggtgacgcga
    12781 cgagaatcga ccagaaaaga gtgcgcaacg tcagccagaa gtgatcctga gtcactttgc
    12841 cgactttcgc cgccgaacgt tcaagaaaac gggtgaaata gcggcgcgag taaatactgc
    12901 aaccgaccag aatcaacgcg ccaaacagcg gcaaaatcgt ctctttgctg gtcagcatca
    12961 tcacactggc tttgcccaac tgactgaagg tgtccagcga aatgagacga cgcagatcct
    13021 gggcgatttc cagcggccag gcgatggtca tcgggcgcac gtcagaggtc cagaacagat
    13081 agcggtgggt cgcttcgttc acttctttca gcgcatcttc cagttgcccg ttggagactt
    13141 tcagcttggt cagttccagc agtagcgtat cgccaccctg caacaatgag ttcagcaact
    13201 cacgctgagt gcgtagctgt gcttccagaa tacggttttg ctcagcggtc agcggctgac
    13261 cgtcggcctg atgaatttgc cgtagcagcg gctgtttatt aagcagatcc tcataacgta
    13321 accgttgcac acgcaactgc gccatttcgg tatcaagctg ttgtggtttc ggcatttccg
    13381 gcagccgtgc cacctgcgcc cgcaacgctt caccgagcag attggacgat cccagccatt
    13441 gcgactgttc acgcagcgta ttcaacgcct gccggacctg taacgtctgg ctggcagcct
    13501 gacgctgttg cgaggcaacg agatccatcc gctgcgcctg ttgattcagc gccgccgata
    13561 gttcgcggtt aattttgaat tgcgcgacga tatctttcgg caaatcggca ctgttttctg
    13621 ctagtaattc ggtactttcc agcgcccgct ctgcctcaag ctggcgttgg ctgttcaatt
    13681 gattacgcaa ggcctgcaaa tacgcatcca gttgctggct ctctttttca gccagctcag
    13741 agcgcaagcg cgctaattcc tggcggttat tggcagacag ctgcgccagc tccagttcat
    13801 caacgagcgc cttaagacgt gcagagtcag actgcaacgc gaaattttgt gcctgattga
    13861 gcggagtatt gccggtaagc gtccccaggc ggcgctcgat ctcatttaac tgacggcggg
    13921 cgtcggtttg ctgttgcggc agttgattca gcgaatcggc aatctcgcgg gcgcgctcct
    13981 gctcttgctg ggcctgacgg cttttatcca gcaactgact gctgacctgg agaatttcct
    14041 gattcagcgc gtcggtagac atccccggcg acacgctgcg cggctcgtca cgcatgttgt
    14101 ttaattgtgc gcgcagagta gcggagagtt tcggataatt atcaatgact tcttgatatt
    14161 gcttgatgcg ctcaagggaa ccttttcgtt cctcaagcgc atttaaggca gactggagtg
    14221 cctctacgac ttctggctgc gcgggtttcg ccgcttttgc ctgctccagt tcctgggtga
    14281 tttgtttgct atcgggggcc gtcgcggcgt acgcccccca actgaggcac caggccatca
    14341 gaaaagtgat aatcaggcgc acgtcagcgt ttcctttgat ggattagacc tggtcttttt
    14401 tatcgtcaac caatgggctg gcgtcgtgtt ctgcttcgat ctcttcagca ggaagcgggg
    14461 cgggttcagc gtctggcgta acaaaggttt cggtagatac tgccagcggc tggccaattt
    14521 tcgtgacaga caggctttcc agttgctcaa ccagattcac tttacccggt gcaaacaggt
    14581 tgataacggt agagccaagt ttaaagcgac ccatttcctg gcctttcagc agcgccacag
    14641 aaccgtcgtt ttccccggca ggccaggtcc agcgcttgat gatgccttcg cgcggcggcg
    14701 taatggtgcc cgcccagacc gtctcaatgc tgcctacaat cgtcgccccg accagaatct
    14761 gcgccattgg gccaaattcg gtatcgaaca ggcagatcac gcgttcgtta cgggcaaaca
    14821 gattcggcac gttctgagcc gtgagatggt taacggagaa gagatcgccc ggcacgtaga
    14881 tcatctcacg cagaataccg ttgcacggca tgtgtacgcg gtggtagtca cgcggggaga
    14941 ggtaagtggt cacaaacgta ccgttgcgga acaggtccgc catcagatag ttgcctgcca
    15001 gcagggcttc gaggctgtag ttgtggcctt tggcttgcag gattttatct tcttcgattt
    15061 tacccaactg gctgataacg ccatcggcag gcatgaccag gacattcgga tcggtatcga
    15121 ttgggcgtac ttcgtcacgc agcggacgga caaagaattc gttaaaggtg cggtagctgg
    15181 cggtgtccgg cttttgcgcc tctttcatgt cgaccttgta gtatttaacg aacagatcga
    15241 taaccagttt tgtcagccat cctgcccgct tgcttgcgcc ccaacccgcc aggcgagtaa
    15301 gccatagttt cggcagaatg tactgtagcg aaagtttaaa tgaatttaac aaggtagcct
    15361 ccaggccatt gttttgtcgt tcctgatccg gcctaaatgc cggatcctga aaaaaagggg
    15421 acgattctaa cgacggttag cttaattgtc agtcatccgt atcagaaaag tttttacgcg
    15481 tttttacctg cgccatgctt tccagaatac ggtgatagtt ttcgaaacgg gtttccgcga
    15541 tttttccttc ctcaaccgct tcccggatag cgcagcccgg atcggtatcg tgtttgcaat
    15601 cgcgatattt acacagacct aaatagtcat ggaattcgac aaagccctga gtgatttgtt
    15661 ccggctccag gtgccagagg ccgaactcac gcacaccagg ggaatcaatc acatcaccgc
    15721 cgtgcgggaa gtgatacagg cgtgcggcgg tcgtggtgtg ctgaccaagc cccgagttgt
    15781 cagagacatc gttggtcagg atctcttttt gcagccccag caacgcgttc agcaggctgg
    15841 atttgccgac gccagactgc ccggcaaaaa tgctgatgcg cccggtcaac gcctcttcca
    15901 gcggttttag cccatcctga gtatggctgg aaaccatcaa tacgcgataa ccgatattgc
    15961 ggtagatatc catctgttcg ttgacgaacg ccatgccttc gtcgtccagc aggtctatct
    16021 tgttgagcac aataatcggc tcaatctgca aggtttcgca ggccaccagg taacggtcga
    16081 taatattgag cgacagctcc ggcaaaatgg cggagacaat gacaatctgg tcgatgttgg
    16141 cggcaatagg tttcacgccg tcgtagaaat ccggacgcgt caacactgag gtgcgctcat
    16201 gcaccgcttc cacgatccct ttgacattta cgccttccgc cgccggttta cccggacgcc
    16261 agactacgcg gtcgccggtc accagcgaac ggatggtacg gcgaatattg cagcggtgaa
    16321 cgtcgccatc ggcggattcc acatcagcgt gcataccaaa gcggctgatg acgatacctt
    16381 catcaggctc gccaaacaga ttgtcgtcgt agtcgggctt ctccttagac gttttaagac
    16441 gacgctggtg attggcgttc acgcggcgct gctggccttt ggagagttta tttttactca
    16501 atcgtactga ctcctggtcg cccctgatgg gcaaaacatc tatgatacac gcaattgtgg
    16561 atcaatatag tcactgtgaa tgggtggaaa atagcatgag tgccaatgaa aacaacctga
    16621 tttggatcga tcttgagatg accggtctgg atcccgagcg cgatcgcatt attgagattg
    16681 ccacgctggt gaccgatgcc aatctgaata ttctggcaga agggccgacc attgcagtac
    16741 atcagtccga tgaacagctg gcgctgatgg atggctggaa cgtgcgcacc cataccgcca
    16801 gcgggctggt ggagcgcgtg aaagcgagca cgatgggcga tcgtgaagct gaactggcaa
    16861 cgctcgaatt tttaaaacag tgggtgcctg cgggtaaatc gccgatttgc ggtaacagca
    16921 tcggtcagga tcgtcgcttc ctgtttaaat acatgccgga gctggaagcc tacttccact
    16981 accgttatct cgatgtcagc accctgaaag agctggcgcg ccgctggaag ccggaaattc
    17041 ttgatggttt taccaaacag gggacgcatc aggcgatgga tgatatccgt gaatcggtgg
    17101 cggagctggc ttactaccgc gagcatttta tcaagctgta aaatttttgc tggtcacgta
    17161 agcgccggaa tccacttgcg gcggcgtgat tttgacgcta aattgaacgt cttgctgata
    17221 atttcagcac ttgagataaa aactcaaaaa aaaattttta ggggggttgc agagggaaag
    17281 atttctcgta taatgcgcct cccgtaacga cgcagaaatg cgaaaattac gaaagcaaaa
    17341 ttaagtagta cgcgggaata gctcagttgg tagagcacga ccttgc
//
LOCUS       DAZFBR010000100        17265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFBR010000100 DAZFBR010000000
VERSION     DAZFBR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.100"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            173..2605
                     /gene="hsdR"
                     /locus_tag="ACHJZ2_003662"
     CDS             173..2605
                     /gene="hsdR"
                     /locus_tag="ACHJZ2_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO4569698.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2672..4141
                     /locus_tag="ACHJZ2_003663"
     CDS             2672..4141
                     /locus_tag="ACHJZ2_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO4569699.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4141..5895
                     /locus_tag="ACHJZ2_003664"
     CDS             4141..5895
                     /locus_tag="ACHJZ2_003664"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO4569700.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
                     QQGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFV
                     CYVENCQTGVAYPAINDKQFFSGITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSL
                     DAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASVDALKQTILQLAVMG
                     KLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCL
                     FEDIIDIQSGITKGRNLSNRTLVKVPYLRVANVQRGYLDLTEIKQIEIPIEEKEKYQV
                     VKGDLLITEGGDWDTVGRTTVWCHDWYIANQNHVFKGRNIGQDVDPYWLETYMNSPFS
                     RQYFANASKQTTNLASINKTQLRGCPVAIPPSSEAKKIMSKLHIFYKLCEELKNHIQS
                     AQQTQLHLADALTDAAVN"
     gene            6116..6457
                     /gene="symE"
                     /locus_tag="ACHJZ2_003665"
     CDS             6116..6457
                     /gene="symE"
                     /locus_tag="ACHJZ2_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO4569701.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
     gene            complement(6504..7667)
                     /locus_tag="ACHJZ2_003666"
     CDS             complement(6504..7667)
                     /locus_tag="ACHJZ2_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO4569702.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(7715..8596)
                     /locus_tag="ACHJZ2_003667"
     CDS             complement(7715..8596)
                     /locus_tag="ACHJZ2_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO4569703.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            complement(8693..8857)
                     /locus_tag="ACHJZ2_003668"
     CDS             complement(8693..8857)
                     /locus_tag="ACHJZ2_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO4569704.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            9034..10446
                     /locus_tag="ACHJZ2_003669"
     CDS             9034..10446
                     /locus_tag="ACHJZ2_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4569705.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(10689..11646)
                     /locus_tag="ACHJZ2_003670"
                     /pseudo
     CDS             complement(10689..11646)
                     /locus_tag="ACHJZ2_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            12113..13345
                     /gene="mdtM"
                     /locus_tag="ACHJZ2_003671"
     CDS             12113..13345
                     /gene="mdtM"
                     /locus_tag="ACHJZ2_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO4569706.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            13386..14657
                     /locus_tag="ACHJZ2_003672"
     CDS             13386..14657
                     /locus_tag="ACHJZ2_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO4569707.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            14773..15924
                     /locus_tag="ACHJZ2_003673"
     CDS             14773..15924
                     /locus_tag="ACHJZ2_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO4569708.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            15934..16701
                     /gene="yjiL"
                     /locus_tag="ACHJZ2_003674"
     CDS             15934..16701
                     /gene="yjiL"
                     /locus_tag="ACHJZ2_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO4569709.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            16698..16964
                     /locus_tag="ACHJZ2_003675"
     CDS             16698..16964
                     /locus_tag="ACHJZ2_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO4569710.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
BASE COUNT         4485 a         4255 c         4194 g         4331 t
ORIGIN      
        1 taacttctgt ttatctgtct ctgatatgcc cagattaatt caagcaagat ataacagaga
       61 tatcaatgtg atctctgcta cgtactttac gttgaaacac cgccctaaat catagccagg
      121 ctattgattt tatgtacaat cagaatcgtc ctgataaaag cgagaatacc ccatggcgga
      181 actgaaccta agtaacctga cggaagcaga catcattacc aaatgcgtta tgccagccat
      241 tctcaatgca ggctgggaca acacaacgca aatcagacag gaggtcaaac tccgggacgg
      301 taaagtcatc gtacgtggta aagttgcggc acgcagaacg gtaaaatctg ctgacatcgt
      361 gctgtatcac aaacctggca ttcccttagc cgtgattgaa gcaaaagcca acaaacatga
      421 aattggcaaa gggatgcaac agggcattga atatgcgcgc ctgctggacg ttccttttgt
      481 ttttgccacc aacggtgatg gctttatctt ccgcgatgcc accgcagccg aaggtgaatg
      541 ccttgaaaag caaataacgc tggatgactt cccctccccc gctgaactct ggcaaaaatt
      601 ctgcctctgg aaaggataca cacaagctca gcttccggtg attactcagg actattacga
      661 cgatggcagc ggtaaatcgc cacgttatta ccagcttcag gcaatcaaca aaaccattga
      721 agccgtctcc aacgggcaaa accgcgttct gctggtcatg gcgaccggaa cagggaaaac
      781 ctataccgca ttccagatca tctggcgcct gtggaaatca aaaaataaaa aacgcatttt
      841 gttccttgcc gatcgcaata ttctggtcga ccaaaccaaa aataatgatt tccagccatt
      901 tggtacggca atgaccaaag tcagcggacg caccattgat cccgcttatg aaattcacct
      961 cgcgctctat caggctataa ctggcccgga ggaagaccaa aaagcgttta aacaagtcgc
     1021 accagatttc ttcgatctga tcgtgatcga cgaatgccat cgcggcagcg catccgaaga
     1081 cagcgcctgg cgagaaatcc ttgattattt tagttccgcc acccaaattg gcttaaccgc
     1141 cacgccaaaa gagacgcatg aagtctccag cacggattac ttcggcgatc cggtttacgt
     1201 ctactcacta aaagaaggga tcgaagacgg cttcctcgcc ccttataaag ttgtccgtgt
     1261 tgatattgat gttgatctgc aaggctggcg cccaaccaaa gggcaaactg acttaaacgg
     1321 cgaagtgatc gacgatcgta tctataacca gaaagatttc gatcgcacga tggtaatcga
     1381 cgaacgcaca gaactggttg ccagaaccat taccgactac ctcaagcgta ccaatccgat
     1441 ggataaaacc atcgtcttct gtaacgacat cgatcatgca gaacgtatgc gccgcgccct
     1501 ggttaatctc aacccggagc aggtgaaaaa gaacgacaaa tacgtcatga aaatcaccgg
     1561 cgatgatgaa attggcaaag ctcagttgga taacttcatc aacccgaaaa aaccgtaccc
     1621 ggttatcgcg accacttcag agctgatgac caccggtgtg gatgctaaaa cctgcaaact
     1681 ggtagtactg gaccagaaca tccagtcgat gaccaaattc aagcagatta tcggtcgtgg
     1741 tacacgcatc gacgaacgtt acggcaaact ctggtttacc atcctcgact ttaaaaaagc
     1801 caccgaactg tttgccgatg agcgtttcga tggcattccc gaaaaagtca tggataccac
     1861 accagaggat atcgccgatc cagaatctga ttttgaagag aaactcgaag aaatcagcga
     1921 acatgacgac gaacaggtaa caggcgttga tgaaccgcct gcgccaccat accaggttaa
     1981 agataccgat gatgtcggcc cacttccgga agaagacgag aagaaaatcc gcaagtttca
     2041 cgtcaacggt gtagcagtgg gcgttattgc ccagcgtgtt cagtattacg acgccgacgg
     2101 taaactggtt accgaatcct ttaaagatta cacccgcaaa acactgctca aagaatatgc
     2161 ctcgctggat gactttaccc gcaagtggca ggacgccgat cgcaaagaag cgatcattca
     2221 cgagctggag caacagggga tcatctggga agtactggca gaagaagtcg gtaaagatct
     2281 cgacccgttc gacatgcttt gccacgtagt gtatggtcag ccgccgttaa cccgcaaaga
     2341 gcgcgccgag aacgtgcgca agcggaacta cttcacaaaa tactctgaag cagcgcaagc
     2401 cgtgctcgat aatctgctgg ataaatacgc cgatgcgggc gtacaggaga tcgaaagtat
     2461 tcaggtgctg aaacttaagc cattcgacag catgggcacc ttaccggaga ttattaaaac
     2521 cggatttggc gaccgtaacg ggtataatca ggcgctcagc gagctggaaa acgaaatcta
     2581 ccaattaccg ccccgctctg cttaacaact ctcttttgcg ccttctgcaa ccatcatgtt
     2641 gcagaaggcc ttgtttttca tggaatacaa catgtctatc agctcagtaa tcaaatcatt
     2701 acaagatatt atgcgcaaag atgccggtgt ggatggcgat gcgcagcgtc tcggtcagct
     2761 ctcctggctg ctgtttttga aaatcttcga tgcccaggaa gaggcgctgg aactggagca
     2821 ggataactat caatatccga tcccacagcg ttatttatgg cgcagttggg ccgccaacgc
     2881 gcagggcatt accggtgatt ccctgctgga attcgttaat gatgatctgt tcccggcgtt
     2941 gaaaaacctc actgcgccta tcgataaaaa cccacgcggc tacgtggtga agcaggcgtt
     3001 cagcgatgcc tataactata tgaaaaacgg tacgctactg cgtcaggtga tcaataagct
     3061 gaacgaaatt gactttacca gcgccagcga acgccatctg tttggtgata tttacgaaca
     3121 gatccttaaa gatctgcaat ctgcgggcaa tgcgggcgaa ttctatactc cacgcgccgt
     3181 cactcgcttt atggtggatc gcgttgatcc gaaactcggc gaatccatta tggacccggc
     3241 ctgcggtacg ggcggttttc ttgcctgcgc atttgatcat gtaaagaaca aatacgtgaa
     3301 gagcgtcgcc gatcatcaga cgctgcaaca acagatccac ggtgttgaga aaaaacagct
     3361 tccgcacctg ctggcgacca ccaatatgct gctacacggc attgaagtgc cagtgcaaat
     3421 tcgtcacgac aacactctga acaaaccgct ttcctcccgg gatgagcaac tggatgtcat
     3481 tgttaccaac ccgccgtttg gtggcacgga agaagacggt attgagaaga actttccggc
     3541 agagatgcaa acccgcgaaa cggcggattt gttcctgcaa ctgattgtgg aagtactggc
     3601 gaaaaatggt cgcgcggcgg tggtattgcc ggatggcaca ctatttggcg aaggcgttaa
     3661 aaccaaaatc aaaaagctac ttaccgaaga gtgcaacctg cataccatcg tgcgcttacc
     3721 gaatggtgtg tttaacccct ataccggcat taaaaccaac ctgttgttct ttaccaaagg
     3781 tcagccaacc aaagagattt ggttctatga gcatccgtat ccggcgggcg taaaaaacta
     3841 cagcaaaact aagccgatga agtttgaaga gtttcaggcg gagatcgact ggtggggtaa
     3901 cgaggccgat ggttttgcca gccgcgtaga gaatgagcag gcgtggaaag tcagcattga
     3961 tgacgtgatt gcgcgtaact tcaatctgga tattaaaaac ccacatcagg cggaaaccgt
     4021 cagccatgat ccggccgagc tgttagcgca gtatgcaaaa cagcaggcgg agatccagac
     4081 gctgcgtaat caactgcgcg atattcttgg cgctgcgctg tctggcaagg aggttaacta
     4141 atgagtgtgg aaaagctgat cgttgatcat atggaaacct ggacctcggc gttgcaaacc
     4201 cgttccaccg ccgggcgcgg cagttccgga aaaattgatt tatatggcat taagaaatta
     4261 cgtgagctga ttctggaact ggctgtgcgc ggtaaactgg tgccgcagga cccgaacgat
     4321 gaaccggcgt cggagctgct gaagcgtatt gcggcagaaa aagcagagct ggtgaagcaa
     4381 ggtaaaatta aaaagcaaaa accactgccg gaaattagtg aggaagagaa gccgtttgaa
     4441 ttgccggagg gatgggagtg ggtgcgtatt agtgaaattg gacatgactg gggacaaaaa
     4501 actccagata aagattttac ttacattgat gttggttcaa taaataaaga atatggaatt
     4561 attgaagagc tatctattct ttcagcgaaa gatgccccat cgcgagcgag aaaaattgtt
     4621 cagcaaggga ctatcatata ctctactgtt cgcccgtatt tgttaaatat agctattatt
     4681 gaaaacgaaa tactacctga accgatcgct agtactgcct tcgctattat tcatccatat
     4741 acggcgatgg atgctaattt catttattat tatctacgtt ctccggtatt tgtttgttat
     4801 gttgaaaatt gtcagacagg ggttgcctat ccagcaatca atgacaaaca atttttttct
     4861 ggaataactc cagttcctcc atccttggaa caagttcgca tcgcaaacaa aatcaaagaa
     4921 ttaatgtccc tctgcgacca actggaacag caatccctga ccagtctgga cgcacatcaa
     4981 caactggttg aaaccctgtt gggaacgctt acagacagcc aaaccgccga ggaactggct
     5041 gaaaactggg cgcgtattag cgagtatttc gacactctat ttaccaccga agccagcgtg
     5101 gatgcgttaa aacagaccat tctgcaactg gccgtaatgg gcaaacttgt gccgcaggat
     5161 ccgaatgatg aaccagcctc tgaactgctc aaacgaattg cgcaggaaaa agctcaactg
     5221 gtgaaagaag gaaaaataaa aaaacaaaaa ccgttgccgc caattagcga tgaggaaaaa
     5281 ccgtttgaac ttccggaagg gtgggagtgg tgtttatttg aagatattat tgatattcaa
     5341 agtggtatca ctaaaggaag aaatttatca aatagaactt tggtaaaagt tccttattta
     5401 cgtgttgcaa acgtccaacg cggatatctt gatcttacgg aaattaaaca gattgaaatc
     5461 cctattgagg aaaaagaaaa atatcaagta gtcaagggag atttattgat aacagaaggc
     5521 ggcgactggg atacagtcgg gagaactaca gtatggtgtc atgactggta tatagcaaat
     5581 caaaaccatg tattcaaagg acgaaatata gggcaagatg ttgatccata ttggttagaa
     5641 acatatatga atagcccatt ctcaagacaa tattttgcta acgcaagtaa gcaaaccact
     5701 aatttagctt ctattaataa aacccagctc agaggttgtc ctgttgctat tcctcctagc
     5761 tcagaagcaa aaaaaataat gagtaaacta catatttttt ataaactatg tgaagaatta
     5821 aagaatcata tccaatccgc ccagcaaacc caactacacc ttgcagatgc actcactgac
     5881 gcggcggtaa actaatttac ctgccctcga aaaaccaata aagcggctaa atagccgctt
     5941 tattcacatc accaaaattt atatttcacg ctaattttca tcttattttc attaattttt
     6001 agctggactt tccccatatt tactgatgat atatccaggt atttagcgcg gtgcggatgt
     6061 gcgccaacac acccgcaccg ctaatcacaa tcactattta tagggaatag cagttatgac
     6121 tgatacgcat tctattgcac aaccgttcga agcagaagtc tccccggcaa ataaccgtca
     6181 attaaccgtg agttatgcga gtcgttaccc ggattacagc cgtattcccg ccatcaccct
     6241 gaaaggtcag tggctggaag ccgccggttt tgccaccggc acggtggtag atgtcaaagt
     6301 gatggaaggc tgtattgtcc tcaccgccca accacccgcc gccgccgaga gcgaactgat
     6361 gcagtcgctg cgccaggtgt gcaagctgtc ggcgcgtaaa caaaggcagg tgcaggagtt
     6421 tattggggtg attgcgggta aacagaaagt tgcctgatac attgacccgg tagcgccagg
     6481 actaccgggc atttttcgct gcgttatacc aacgcgctct ccatctgttt ttcaaatgcc
     6541 tgacgaatta accccataat gtaaggaagt tgtgcgaggt cactgaaacc aatttccaca
     6601 tcaccattgc cccatcgccc aacatttgtc acatctttgg caataccttt cggatcgacc
     6661 agttcatgaa actgcatatt aagcgacaat cgcagtcggc ttttttgcgg cacaacatca
     6721 acaaaattcg tttccgcttt aaacgcaata tagagcttca atacttcctg cgtgatccct
     6781 gcgtccaggc gcataatttc atcgcgaaga tgatcgaata acacccggct atgcgaacca
     6841 tcagcaagaa aaggataatc actcaggctg taactggttt tgttctcagg ctgtccccga
     6901 taaacagcta aaacctcttc aggaagagaa ggcgcttgcc agacctgaac cgcaagctcc
     6961 gccagacgat cggctcgggc gtgaatggca gcttcatccc atttttcgca ctgtccaaga
     7021 ccgatattca aatagagcgg gctatgctta aagccgtctt caatatcgcg tttttctgcg
     7081 aaaggtctgt cactgtaacg ggagttatag cgcgttaaag tgagattccc caacgtatgc
     7141 aacagttctt tatgaatacg ctgccagtca cttcccagct cttcgcgcca tttagccgac
     7201 aaattttcgt tctgcggcat aatatgttca atcgtaaact cttccacgcg ctctctgcgt
     7261 ttatcgtttt ccagtcgtcg taaccagtag ctacgactac ggaagttata gagatcgcga
     7321 acttttaatt cccgtttaaa ttcatcatcg ttggggaaac gacgatatga aggtagattc
     7381 aaaaaatgta cctgaatact ttccagatat ttttctttat taatgacctt ataaaaagta
     7441 gcaaacgtct tgttaagaga atttgtcgga atcgcacata ctgcacggcg gaaaacataa
     7501 gattcaatta aacgaattat gctcaggaaa tcttcgtgag acaaatcgtc atttttatag
     7561 tcatgataaa gcgccagtaa gaaaggatac gccacatcaa cctttaactc gcgcaaatcc
     7621 tgaaaagccg tagcaagcga tttgtcactt tcttttccca atgccattgc acagtaatac
     7681 tccgcgtaga tatgaatatc ttcaaccagc cgatctacgc ctttttcagc aacactctgg
     7741 ctgcgggcat ggagtttaaa tgcctcatag acttcatctg tccgagggat ctccccgttt
     7801 ttaccgtcag ataatgacgc ataaaactgt caaaatattc gctgtaacct tgctgaccac
     7861 aagcgacttc cattggacgc cagtgatctt catacaaccg ggtttgatgc tctggttcga
     7921 ggcccatcag aataaaattg cgcaccagat cggcctgact taacgcctta ccggtggagt
     7981 tcatactttc aaaaatcagt tgcggattat cctgaccacg attaagcgcc acatcgacaa
     8041 ttaataactt tgctaaccca cgacataagg ggatcaagtc atcaccgagt ttggcaattt
     8101 gttcatcaaa gaaagtaaag ttttccatta ttcgatggga atagttttct ggcattggtc
     8161 tttgttttat taacgccagt aaactgtctt tatcagtctc ggtcagcagt aatttaaaac
     8221 ctttctcgcc ggactcataa ggattgagca aataataatt acgcaatttc attgccgaaa
     8281 agccatcaaa aacttcgtct tcgccaagat ggcgcgataa agcctcaatc agcaacattg
     8341 cggtcgtcag ccgctgctga ccatcaatga ccagcaacgg agaaatacca gaaacctgat
     8401 acaatccctg ctctatataa acaaccgaac cgataaaatg cgctgatata tcgtcacgct
     8461 ttcctgcacg aatgatgtcg tcccaaagtt gccgacattg ttgttctgtc catgaataag
     8521 tccgttgata aatgggaata acaaactgct gcgaacgttt taaaaaatcg agtaggcgag
     8581 cttctgtagc tttcattctg acgtctttcc gtgaaacgat gatttcgcaa taataaccac
     8641 ttccttatgg aggttataga gaaatcctgt ttatccgcga cgaacatacc cctcactcca
     8701 catcctccct gcgtaaccca atatccctca accgttcatc actcatctgc tgtaacaccc
     8761 gtcgggcctg catttgccgc cgccaacgcc tcactgcctg ccagagttgc acaagtctga
     8821 taaacggcgt tttggctttg ttttcgtgaa attccatctg ctgcctcctc atatttcaga
     8881 ggcttcatca tccgtcttct ggcagttcac aatacagatg caaatattag ttttgtttaa
     8941 catacagaca ggttaaaaca gcatctgcat ccggtttttt agcctcatct gtaccggttt
     9001 ttctttctgt atggtccaaa taaggaatac agcatgacgc gttatcaaca tctggcgact
     9061 ctacttgccg agcggattga gcaagggctg tatcgtcacg gggagaaatt gccgtcggtg
     9121 cgcagcttaa gtcaggagca cggcgtcagc atcagcaccg tgcagcaggc gtaccagacg
     9181 ctggagacga tgaagctcat cactccgcag ccgcgttcgg gttattttgt cgcacaacgt
     9241 aaagcccagc cgccagtgcc gccgatgacg cgtccggtgc agcgcccagt ggaaattacc
     9301 cagtgggatc tggtgctgga tatgctggtg gcgcatagcg acagttccat tgttccgtta
     9361 agcaaaagca cgccggatgt cgaaacgccc agcctgaaac cgctgtggcg ggagctaagc
     9421 cgggtggtgc agcataatct gcaaaccgtt ctcggttatg acttgctagc cggtcagcgg
     9481 gtattgcgcg agcaggttgc ccgcctgatg ctcgacagcg gctcggtggt cactgccgat
     9541 gacatcatca tcaccagcgg ctgccataac tcgatgtcgc tagcgttaat ggcggtgtgt
     9601 aaaccgggcg atattgtcgc ggtcgaatcc ccctgttatt acggttcaat gcagatgctg
     9661 cgcggcatgg gcgtgaaagt gattgaaatc ccaaccgatc cagaaacagg catcagcgtt
     9721 gaagcgctgg aactggcgct ggaacagtgg ccgattaaag gcatcattct ggtgccaaac
     9781 tgtaataatc cgctgggatt tattatgccg gacgcgcgca aacgggccgt tctctctctc
     9841 gctcagcgtc atgatattgt gatttttgaa gatgatgtct acggcgaact ggcaacggag
     9901 tatccgcgcc cgcggaccat tcattcctgg gatatcgacg ggcgagtgct gttgtgcagc
     9961 tcgttcagta aaagtattgc tccaggcctg cgcgtgggtt gggtcgcacc ggggcgttat
    10021 cacgataaac tgatgcatat gaaatacgcc atcagcagct ttaatgtgcc gtccacgcaa
    10081 atggcggcgg caacgtttgt gctggaaggt cactatcatc gccatatccg gcggatgcgg
    10141 cagatctatc agcgcaattt ggcgctttat acctgctgga tacgggaata ttttccctgc
    10201 gaaatctgta ttacgcgccc gaaaggcgga tttttactgt ggatagaatt gcctgaacag
    10261 gtcgatatgg tctgcgtcac gcggcagctg tgccgcatga aaatccaggt ggcggcaggc
    10321 tcgattttct cagcttccgg caaataccgt aactgcctgc gcatcaactg tgctttgcca
    10381 ctcagcgaaa cttatcgcga agcgctgaag caaattggcg aggccgtgta tcgggcaatg
    10441 gaataaatta gaattagtgg cgaagcacct gatttattct cacgaatcgc ctgtgactcg
    10501 ttttatcttt gcaggatcat agccacgttc cggcatggct taggtaaagt gcgctttgtt
    10561 catgccggat gccatgtgaa cgccttacca agtctacaaa atctttaaag ttcaatatat
    10621 tgaatgggct ttgtagacct gataagcgta gcacatcagg ctgctttgcg tttgtcatcc
    10681 gtctctggtt agctcttgag ccatgaatag cgctcacgct caggcaaggg ctgccttaat
    10741 gcctgcattt gctctgccga aagtccggta atcttctgta tccattcagg atctgcccca
    10801 ttctgcaaca acaatcgaag aatgcgctgt tccccttctt cccttcccaa cagccatcca
    10861 tcattatgaa ttcgctctgc aatcgtcatt atgaattcgc tctgcaatcg tcattattcg
    10921 ctctctgtgt tgaggcattc gactggttag ttcactgata aactcattaa aacgctcttc
    10981 atcgccagtc agtaaaatgt aatttaacag cgcagttatc tggctgtcat tagcacactc
    11041 tgtaaccagt aatactacca gttgatcgat aagccccatc agatcgcgct ggcgaatatg
    11101 cttttggatc aactccaaca gggcgactct gcggtgctgc acaatctcgt cgtccggcac
    11161 gacagtgata tccaccagcg ggaacgcggc gttataaagc ttccgtgcag tagtcgggtc
    11221 ggcaaattcg tccaaccagc acaaagacca gggataagga ctacggctac cgtgataaaa
    11281 cagcatcggg atgaccagcg gtagtggccg gcgtttatca tgctctatat ggcgctgcat
    11341 cactgccatg gaatagcgca tcaggcgaaa ggccatatga atatcctcac ggctctgatg
    11401 ttcaatcacc acatagatat agccatcgcc ttcccgggtc tttaccgacc acagaatatc
    11461 ggagtgtagc gcccgcaatt tttcatcgac aaagctggcg gattccagtt ttaagctgtc
    11521 gagatcgcac agttcacgta aatctttggg taagtggatc tccataaaat cccgcgcggt
    11581 gtcagggtgc atgagaaaag atttaaataa tgcgtcatgc ggcgtgctgg tcgtgaagtt
    11641 tgtcatggcg atccgtcacc tgttgataag atggcccgac tttagcgact aacaaaacca
    11701 ttctcacccg gcaatttctc gtttcccgag gcgctttcca ggctattttt gttcgtctgc
    11761 aaacgataca tttttccgga aactgactcg cgttttacat gtcctgtacc cggcagatat
    11821 tcccccttcc accgcgctcc tttgattaat cgcaaatttt aattacactt aaggtgtata
    11881 ttttctatgc aacccatcaa ttcaagaggt gtaatgtgct gatgactatt tgtaatcgtt
    11941 atacattctg acccgaagtc agaaggtatt tctctgtctg tgtgttcaca ggcagtgtgg
    12001 ttgattacat gaaattcagt acatttgtcc ttctcgccta cctttcgtca ttaccgacgg
    12061 tattgaattt cgttttcccc gttggggttc tccggacaag gagttgtttg ttatgccacg
    12121 tttttttgcc cgccatgccg ccacgctgtt tttcccgatg gcgttgattt tgtatgactt
    12181 tgccgcctat ctttcgacgg acctgatcca gcctgggatc attaatgtgg tccgtgattt
    12241 taatgccgat gtcagtctgg ctccggctgc cgtcagtctc tatctcgctg gaggcatggc
    12301 gttgcagtgg ctgctggggc cgctttccga caggattggc cgcaagccag tgctgattac
    12361 cggggcgcta atatttaccc ttgcctgcgc tgcgacaatg ttcacaacgt ctatgacaca
    12421 gtttcttatc gcgcgtgcaa ttcagggcac cagtatctgt tttattgcca ccgttggtta
    12481 tgtcacggtg caggaggcgt tcggccagac aaaagggatc aagttgatgg cgattatcac
    12541 ctccatcgta ctgattgcgc cgattatcgg cccgctttcc ggcgcagctc tgatgcactt
    12601 tgtgcactgg aaagtccttt ttgccatcat tgcggttatg ggttttatct catttgttgg
    12661 cttactgctg gcgatgccag agacggtgaa gcgcggcgcg gttccattta gcgccaaaag
    12721 cgtcttgcgc gattttcgta atgtcttttg caatcggctg ttcctctttg gcgcagcaac
    12781 catctcttta agctatatcc ccatgatgag ttgggtggct gtctcgccgg tgatcctgat
    12841 tgacgcaggc ggcttaacga cctcgcagtt cgcctggaca caggttccgg tgttcggcgc
    12901 ggtgattgtc gcgaatgcca tcgtggcgcg tttcgttaaa gatccgaccg aaccgcgctt
    12961 tatctggcgt gccgtaccca ttcaactggt cggcctcgcg ctgttgatta tcggcaatct
    13021 gctgtcaccg cacgtctggc tgtggtcggt gctgggcacc agtctgtatg ctttcgggat
    13081 tggtttgatc ttcccgacct tattccgctt tacgctgttt tccaataact taccgaaagg
    13141 gaccgtctcc gcatcgctga atatggtgat cctgatggtg atgtctgtct cggtcgaaat
    13201 tggccgctgg ctgtggttta acggcggtcg cttgccgttt catctgttag ccgttgtggc
    13261 gggcgttatc gtcgttttca ccctggccgg attgcttaat cgcgtgcgcc aacatcaggc
    13321 tgccgagctg gcagaggagc agtgattttt gcgcgatcct gccgtcaggc tctattctta
    13381 acgttatgaa taaactcatt gaactcagac gcgccaaaat gttggcgctc tctttactgc
    13441 ttatcgccgc tgctaccttt gtcgttacgc tgtttttgcc gcccaatttt tgggtgagcg
    13501 gcgtgaaggc gattgctgaa gcggcgatgg tcggcgcgct ggcggactgg tttgcggtgg
    13561 tggcgctgtt tcgccgcgtg ccgattccga tcatttctcg tcatacggcg attatcccgc
    13621 gtaataaaga ccggattggc gaaaatctcg gtcagttcgt gcagaaaaaa tttctcgata
    13681 cccaatcgct ggtggcattg attcgacgtc acgaaccggc gttgctgatt ggcaactggt
    13741 ttagccagcc agaaaacgcc cgccgcgttg gtcagcatct gttgcagatc atgagtggtt
    13801 ttcttgaact gaccgatgat gcacgtattc agcgcctgct taagcgcgcg gtccatcggg
    13861 cgattgataa ggtcgatctt tccggcacca gtgcgttgat gctggagagt atgaccaaaa
    13921 acgatcgtca tcaggtgctg ctggatacgc tgatcgcaca gttgatcgcc cttctccagc
    13981 gcgataaatc gcgcaagttt atcgcccagc agattgttcg ctggctggag agtgagcatc
    14041 cactgaaagc caaaattctc cccaccgaat ggctgggcga acatagcgcg gagttggttt
    14101 ctgacgcggt gaattctttg cttgatgata tcagccgcga tcgtgcgcat cagatccgcc
    14161 atgcatttga tcgcgccact tttgccctga tcgacaagtt gaaaaacgat cctgaaatga
    14221 cagcgcgagc tgatgccgta aaaagctatc tgaaagagga tgaagccttt ctcagtgaat
    14281 tgtgggggga tttacgggaa tggctgaaag cggatatcaa cagtgaagat tctcgtgtga
    14341 aagaacgtat cgcgcgggca ggtcaatggt ttggcgaaac gttaattgcc gatgatgcct
    14401 tgcgggcgtc gttaaatggt cacctggaac aagccgcgca ccgcgtcgcg cctgagtttt
    14461 ctgcattcct gacgcgccat atcagcgata cggtaaaaag ctgggatgcc cgggatatgt
    14521 cgcggcaaat agagttaaat atcggcaaag atctgcagtt tatccgtgtc aacggtacgc
    14581 tggttggcgg ttgtattggg ctaattttat atttgctgtc acagctcccg gccttgttcc
    14641 ccctcggcaa tttttagaaa tccatgaaaa actaagtggc taaccgcgag agagatcaaa
    14701 taaaccatac taaaagaatg gtaataatgt ggcctctttt cattatctcc cgtggtacgg
    14761 ggaaggaaaa tcatgtcact tgtcaccgat ctacccgcca ttttcgatca gttctctgaa
    14821 gctcgccaga aaggctttct caccgtcatg gatctcaagg tgcgcggcat tccgctggtt
    14881 ggcacttact gcacctttat gccgcaagag atcccgatgg cagccggtgc ggttgtggtt
    14941 tcgctctgtt ccacctctga tgaaaccatt gaagaagcgg agaaagatct gccgcgcaac
    15001 ctctgcccgc tgattaaaag tagctacggc ttcggcaaaa ccgataaatg cccctacttc
    15061 tacttttcgg atctggtggt cggtgaaacc acctgcgacg gcaaaaagaa aatgtatgaa
    15121 tacatggcgg agtttaagcc cgttcatgtg atgcagctgc caaacagcgt taaagacgat
    15181 gcctcgcgtg cgttatggaa agccgagatg ctgcgcttac aaaaagcggt ggaagaacgt
    15241 tttgggcacg aaattagcga agatgctctg cgcgatgcca ttgcgctgaa aaaccgcgaa
    15301 cgtcgcgcac tggccaattt ttatcatctt gggcagttca atcctccggc gcttagcggc
    15361 agcgacattc tgaaagtggt ttacggcgca accttccggt tcgataaaga ggcgttgatc
    15421 aatgaactgg acgcgatgac cgcccgcatt cgtcagcagt gggaagaagg ccagcgactg
    15481 gacccgcgtc cgcgcatttt aatcaccggc tgcccgattg gcggcgcagc agagaaagtg
    15541 gtgcgcgcga ttgaagagaa tggcggctgg gttgtcggtt atgaaaactg caccggggcg
    15601 aaagcgaccg agcaatgcgt ggcagaaacg ggcgatgtct acgacgcgct ggcggataaa
    15661 tatctggcga ttggctgctc ctgtgtttcg ccgaacgatc agcgcctgaa aatgctcagc
    15721 cagatggtgg aagaatatca ggtcgatggc gtagttgatg tgattttgca ggcgtgccat
    15781 acctacgcgg tggaatcgct ggcaattaaa cgtcatgtgc gtcagcagca caacattcct
    15841 tatatcgcta ttgaaacaga ctactccacc tcggatgttg ggcagctcag tacccgtgtc
    15901 gcggccttta ttgagatgct gtaaggagta gcagtgacat attcgattgg cattgattct
    15961 ggttctaccg ccaccaaagg gatcttactc gcagacggcg tgattacgcg ccgtttcctc
    16021 gttccaacac cctttcgacc ggcaacagca attactgaag cctgggaaac tctgcgcgaa
    16081 gggttagaga caacgccgtt tctgacgctc accggctacg ggcggcaact ggtggatttt
    16141 gccgataaac aagtgacgga aatctcctgt cacgggctgg gcgcacggtt tcttgcgcca
    16201 gcaacgcgcg cggtaatcga catcggtggt caggacagca aagtgattca gcttgatgat
    16261 gacggtaacc tgtgcgattt cctgatgaat gacaaatgcg cggcgggcac cgggcgtttc
    16321 ctggaggtga tctcgcgcac gcttggcacc agcgtcgagc aactcgactg cattaccgaa
    16381 aatgtcacgc cgcacgccat cacgagtatg tgcacagtgt ttgctgaatc agaagtgatc
    16441 agcctgcgct cagcgggcgt cgcgccagaa gcgattctcg ccggagtgat taacgcgatg
    16501 gcgcggcgga gtgccaattt cattgctcgt ctctcctgtg aagcgccgat tctgtttact
    16561 ggtggcgtta gtcattgcca gacgtttgcc cggatgctgg aatctcacct gcgaatgccg
    16621 gtaaataccc atcctgatgc gcaatttgct ggcacaattg gcgcggcggt aattggtcaa
    16681 cgagtgagga cacgccgatg aaagagtttt tatttttatt tcactccacg gtcggcgtca
    16741 tacaaacccg caaggcgttg caggcagcgg gcatgacttt tcgcgtcagc gatattcccc
    16801 gtgatttacg cggcggctgc gggttatgca tttggctgac ctgtcccccc ggcgaggaaa
    16861 tacaatgggt gatccctggg caaaccgaag cgatatattg ccagcagggc agcgactggc
    16921 agtgcgtggt gcattacgat gcagaagcgt caaccaggca ataggatctt taaatatacc
    16981 aaccggataa ttggctgcgc ataatcctgc atgaaggaag ttgtagtaaa tatctcgcct
    17041 gccgtgaaat atttcccatc cacaaaatga gcgatttttg atagcacctt cctattgcgc
    17101 gtctaaaata caatatacat tgaattatac ttaaacccat aatcatgcat agagaaataa
    17161 aatgattacc gggtgttgta atgttaaaag ataagtttct gtgcaaagct tatctttccc
    17221 caccccctga ttattttaga tttagaagaa tatttcacag ttcaa
//
LOCUS       DAZFBR010000101        16922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFBR010000101 DAZFBR010000000
VERSION     DAZFBR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.101"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..970
                     /locus_tag="ACHJZ2_003676"
     CDS             <1..970
                     /locus_tag="ACHJZ2_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569711.1"
                     /translation="EFFCDEFLKISQSRYGLSTSTDSIANLLAFFTKASDAIDSIKTQ
                     KIDVHSYGFVPIRHFVEHVITYKNEFAADYEPYTLTFTRGENNEGILSVESKEGFISQ
                     RTINLNEYETAINIINEHVIKENIHNTVQSPTEKYISKINSSDKPYKTSSKESIKSQL
                     YSDQRNIQTCYYILRKNTEWYKYASSEERYDKFKNSSKEIKNTYKQIVQAQKKLNQMK
                     YINKLSGELIDITDKKLAPLINDSFSYTRDFFAYSKQEKNIFTFDNSKFVDPKEKEGL
                     MVQHSNGQLVITGKYCPEGVQTAFTQEQYDKLIRYINIFFTFPKLE"
     gene            complement(1194..2270)
                     /gene="glpQ"
                     /locus_tag="ACHJZ2_003677"
     CDS             complement(1194..2270)
                     /gene="glpQ"
                     /locus_tag="ACHJZ2_003677"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4569712.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(2275..3633)
                     /gene="glpT"
                     /locus_tag="ACHJZ2_003678"
     CDS             complement(2275..3633)
                     /gene="glpT"
                     /locus_tag="ACHJZ2_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO4569713.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            3906..5534
                     /gene="glpA"
                     /locus_tag="ACHJZ2_003679"
     CDS             3906..5534
                     /gene="glpA"
                     /locus_tag="ACHJZ2_003679"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO4569714.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            5524..6783
                     /gene="glpB"
                     /locus_tag="ACHJZ2_003680"
     CDS             5524..6783
                     /gene="glpB"
                     /locus_tag="ACHJZ2_003680"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO4569715.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            6780..7970
                     /gene="glpC"
                     /locus_tag="ACHJZ2_003681"
     CDS             6780..7970
                     /gene="glpC"
                     /locus_tag="ACHJZ2_003681"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO4569716.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            8163..9089
                     /gene="rpnE"
                     /locus_tag="ACHJZ2_003682"
     CDS             8163..9089
                     /gene="rpnE"
                     /locus_tag="ACHJZ2_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO4569717.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(9130..9924)
                     /gene="yfaU"
                     /locus_tag="ACHJZ2_003683"
     CDS             complement(9130..9924)
                     /gene="yfaU"
                     /locus_tag="ACHJZ2_003683"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO4569718.1"
                     /translation="MINHPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDG
                     EHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQA
                     RQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDE
                     ILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPD
                     MAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(9950..11239)
                     /gene="yfaV"
                     /locus_tag="ACHJZ2_003684"
     CDS             complement(9950..11239)
                     /gene="yfaV"
                     /locus_tag="ACHJZ2_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4569719.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKLEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLLGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(11296..12501)
                     /gene="rhmD"
                     /locus_tag="ACHJZ2_003685"
     CDS             complement(11296..12501)
                     /gene="rhmD"
                     /locus_tag="ACHJZ2_003685"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO4569720.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQLRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(12516..13298)
                     /gene="yfaX"
                     /locus_tag="ACHJZ2_003686"
     CDS             complement(12516..13298)
                     /gene="yfaX"
                     /locus_tag="ACHJZ2_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO4569721.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(13518..14720)
                     /gene="yfaY"
                     /locus_tag="ACHJZ2_003687"
     CDS             complement(13518..14720)
                     /gene="yfaY"
                     /locus_tag="ACHJZ2_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO4569722.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(14820..15362)
                     /gene="yfaZ"
                     /locus_tag="ACHJZ2_003688"
     CDS             complement(14820..15362)
                     /gene="yfaZ"
                     /locus_tag="ACHJZ2_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO4569723.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            15641..16066
                     /gene="nudI"
                     /locus_tag="ACHJZ2_003689"
     CDS             15641..16066
                     /gene="nudI"
                     /locus_tag="ACHJZ2_003689"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO4569724.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            complement(16105..16707)
                     /gene="ais"
                     /locus_tag="ACHJZ2_003690"
     CDS             complement(16105..16707)
                     /gene="ais"
                     /locus_tag="ACHJZ2_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO4569725.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLGRLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
BASE COUNT         4457 a         4457 c         4026 g         3982 t
ORIGIN      
        1 tgagtttttt tgcgatgagt ttctaaaaat atctcaaagt agatacgggt tatcaacctc
       61 aacagacagc atagctaatt tattggcttt tttcacgaaa gcctcagatg caattgatag
      121 tatcaagacc caaaaaattg acgttcacag ttatggtttc gtaccaataa gacattttgt
      181 cgaacatgtc ataacgtata aaaatgaatt tgcagcggat tatgaaccct atacccttac
      241 ctttacccga ggtgaaaaca acgaaggtat tttgagtgtc gaaagcaaag aagggtttat
      301 ttcacaaaga acaataaact taaatgaata tgaaactgca attaacatca tcaatgaaca
      361 tgttataaaa gaaaatatcc acaacacggt ccagtcaccg acagaaaaat acatttcaaa
      421 aataaacagc tctgataaac cttataaaac cagcagtaag gaaagtatta aaagtcaact
      481 ttacagcgat caaagaaata tacagacctg ctactacatt ctgagaaaaa atacagaatg
      541 gtacaaatac gcatcctccg aggaacgcta tgataaattc aagaattctt ctaaggaaat
      601 taaaaatacc tacaagcaaa ttgtacaagc gcagaaaaaa ttgaaccaaa tgaaatacat
      661 caataaatta agtggtgagc ttatcgatat caccgacaaa aagttagcac cattaataaa
      721 tgatagtttc agctatacac gagatttctt tgcttactca aaacaggaaa aaaatatctt
      781 cacatttgat aattctaagt ttgtagatcc aaaggaaaaa gaaggtttaa tggtacaaca
      841 cagcaatggg caactggtta ttactggtaa atattgccct gaaggtgtgc aaacagcatt
      901 cacccaagaa caatatgata aattaataag atacattaat attttcttta cctttccgaa
      961 attagaataa tactttacag tataaactgc attacaattt attattgaga cctattcata
     1021 ctgtttaaag aataacaatg cccagtgtta gcttttttaa cgaatgagtt ttacgaaaat
     1081 tatgttggca tcttttttac aggacgcacc ggttaatgaa aagaataacc ggtagtagca
     1141 ttatttcgct ttagcagagg atggcgcatg aacgccatcc tgcagtagca gcattactct
     1201 ttattaagga atttaaccgc tttatcaggg aaatcagtaa acaacccatt tacacccgct
     1261 ttgttataca gagcatcata taactgattc acatcagttg tgtattcagg cagtttatct
     1321 gaccgcacgg tataaggatg cactaccagc ttgttctgct gagcatcttg caccatgcca
     1381 gtgagtttga tattacccgg ctgcgatgtc tcctcaatca acatatggta atccggacca
     1441 ataccatctg catattccgc cacctgtttc atggcacccg gcttaaacat ccagtcgtag
     1501 ttgtaattaa cccagcttcc gtccggctgt ttctgctgcg tttcattcca gtcggtatag
     1561 gcaatcagct gtaccagatt gagatccatg cccattttgg gttccagctc attcttaata
     1621 cgcttcagct catcagcatc aaaacattgc aaataaactt tatcgtcttt accggtgtaa
     1681 ccatatttct tcagcacttc cagcgttttt gccgcaatat ccttcccttc ctgatgatgg
     1741 aaccacggcg ctttgatttc tggatagata ccgatatttt tcccggtaga gtgatttaac
     1801 ccctgaacaa attcaatctc ttcttcaaag gtgtgcaccc ggaagtcgga cttacccatt
     1861 gggaaacgcc ctggataagt ctgcactttt ttaccgtttt caatatcgaa accttcggta
     1921 aatttcaacg acttaatttc atccagcgtg aaatctatcg cgtagtaacg accgtctttg
     1981 cgcgcccgat ccgggaaacg atcggcaaca tcagtaacac gatcgaggta atggtcatgc
     2041 agaacaacca gatggtcgtc tttggtcatc accaaatcct gttccagata atccgctccc
     2101 tgcgcatacg ccatcgcttt tgctggcagc gtatgctccg gcaaatatcc actggcaccg
     2161 cgatgggcga tgactatttt ttcgttgctg tccgccgcca ttgcactgct tcccatgact
     2221 atagtgctca tcatgatcgc catgctaagg tttttcagcg tcaatttcat gccattagcc
     2281 tccgttgcgt ttttgcagta attcctgatg gcgacgtttt tcgccaatca tcacaacaat
     2341 caacaagata accgctagaa tgctgccgcc aatcattacc ataaagccgc catcccagcc
     2401 gaagaagtcc acggtgtagc caacaatcgc gctcgccgcc accgaaccgc ccaggtaacc
     2461 aaacagaccg gtaaagcccg ctgccgttcc tgccgctttt ttcggtgcca gttccagcgc
     2521 atgcagaccg atcagcatca cagggccgta gatcaggaag ccgataacaa tcatacaaat
     2581 catatcgacg gttgggttac ctgccgggtt catccagtaa acgatagtcg cgatggtcac
     2641 cagtgtcata aagaaaacgc cggttgcccc acggttgcca cggaagactt tatccgacat
     2701 ccagccgcac agcagggtgc ccggaatacc cgcatactca tagaagaagt aggcccagga
     2761 ggatttatcc agcgcgaaat gcttaacctc tttcagataa gtcggtgacc agtcgaggat
     2821 gccgtaacgc agcagataaa cgaacacgtt ggcgatggcg atataccaca acagtttgtt
     2881 cggcagcacg tactgcatga agatttgctt cgcggtcagc tcctgttccg ctttttcgtt
     2941 atagtcgtcc ggataatcat ttttgtactc ttcgatcggc ggcaagccac aggattgcgg
     3001 ggtatcgcgc atcatcgcaa aggcgaataa tgccaccaga atggcgcaga aagcgggcat
     3061 atagagcgcc gcgtgccagt cattgaacca ggccatcccc agcaggaaca gcagcggcgg
     3121 aataccacca ccgacgttgt gcgcacagtt ccacactgac acaatgccgc cacgttcttt
     3181 ctgcgaccac cagtgcacca tagtacgacc gcatggcggc caccccatcc cctggaacca
     3241 accgcagagg aacaacagta caaacatcac cgcaatgctc gacgtcgccc atggcacaaa
     3301 gcccataaac aacatcactg ccgccgccag aatcaaacct gcgggcagga aaacgcgcgg
     3361 attcgagcga tccgataccg aacccatgat gaatttcgaa aatccatagg cgatcgagat
     3421 cccagaaagc gcaaaaccta aatcaccgcg tgagaatccc tgctccacca ggtaaggcat
     3481 agcgagcgca aagttcttac gaaccaaata gtaagcagca tagccaaaga atatccccag
     3541 gaaaatttgc cagcgcaatc gacgataagt cggatcgatc tccgcggcag gtaagcgcgc
     3601 tttgtgtggc gctggtttaa aaatactcaa cattgaaagc ctccgtggcc cgtggtctta
     3661 tttatgatta acagcctgat tcagtgagag aacctgccgt ttcttgagtt gccgcgatgt
     3721 taagaaaaca ttcataaatt aaatgtgaat tgccgcacac attattaaat aagatttaca
     3781 aaatgttcaa aatgacgcat gaaatcacgt ctcactttcg aattatgagc gaatatgcgc
     3841 gaaatcaaac aactcatgtt tttactatgg ctaaatggta aaaaacgaac ttcagaggga
     3901 taacaatgaa aactcgcgac tcgcaatcaa gtgacgtgat tatcattggc ggcggcgcaa
     3961 cgggagccgg aattgcccgc gactgtgccc tgcgcgggct gcgcgtgatt ttggttgagc
     4021 gccacgacat cgcaaccggt gccaccgggc gtaaccacgg cctgctgcac agcggtgcgc
     4081 gctatgcggt aaccgatgcg gaatcggccc gcgaatgcat tagtgaaaac cagatcctga
     4141 aacgcattgc acgtcactgc gttgaaccaa ccaacggcct gtttatcacc ctgccggaag
     4201 atgacctctc cttccaggcc acttttattc gcgcctgcga agaagcaggg atcagcgcag
     4261 aagctataga cccgcagcaa gcgcgcatta tcgaacctgc cgttaacccg gcactgattg
     4321 gcgcggtgaa agttccggat ggcaccgttg atccatttcg tctgaccgca gcaaacatgc
     4381 tggatgccaa agaacacggt gctgttatcc ttaccgctca tgaagtcacg gggctgattc
     4441 gtgaaggcgc gacggtgtgc ggtgttcgtg tacgtaacca tctcaccggc gaaactcagg
     4501 cccttcatgc acctgtcgtg gttaatgccg ctgggatctg ggggcaacac attgccgaat
     4561 atgccgatct gcgcattcgc atgttcccgg cgaaaggatc gctgctgatc atggatcacc
     4621 gcattaatca gcatgtgatc aaccgctgcc gtaaaccttc cgacgccgat attctggtgc
     4681 ctggcgatac catttcgctg attggcacca cctctttacg tattgattac aacgagattg
     4741 acgataatcg agtgacggca gaagaggttg atattctgct gcgtgaaggg gaaaaactgg
     4801 cccccgtgat ggcgaaaacg cgcattttgc gggcctattc tggcgtgcgc ccgctggttg
     4861 ccagcgatga cgacccgagc ggacgtaacg tcagccgtgg catcgtgctg ctcgaccatg
     4921 ctgaacgcga tggtctggac ggatttatca ccatcaccgg tggcaaactg atgacctatc
     4981 ggctgatggc tgaatgggct accgacgcgg tatgccgcaa actgggcaac acgcgcccct
     5041 gtacgaccgc cgatctggca cttccgggtt cacaggaacc cgctgaagtt accttgcgta
     5101 aagtcatctc cctgcctgcc ccgctgcgcg gttctgcggt ttatcgtcat ggcgatcgca
     5161 cgcctgcctg gctgagcgaa ggccgtctgc accgtagcct ggtatgtgag tgcgaagcgg
     5221 taactgcggg tgaagtgcag tacgcggtag aaaatttaaa cgttaatagc ctgctggatt
     5281 tacgccgtcg tacccgtgtg gggatgggta cctgccaggg cgaactctgc gcctgccgcg
     5341 ctgctggact gctgcaacgt tttaacgtca cgacgtccgc gcaatctatc gagcaacttt
     5401 ccaccttcct taacgaacgc tggaaaggcg tgcaacccat cgcctgggga gatgcactgc
     5461 gcgaaagcga atttacccgc tgggtttatc agggactgtg tggtctggag aaggagcaga
     5521 aagatgcgct ttgatactat cattatgggc ggcggcctcg ccggattact ctgtggcctg
     5581 cagctgcaaa aacacggcct gcgctgtgcc attgtcacgc gtggtcaaag cgcgctgcat
     5641 ttctcatccg gttcgctgga tttgctgagc catctgccag atggtcaagc tgtgacagac
     5701 attcacagtg gactggaatc tttgcgtcag caggcacctg cacatcctta ctctcttctc
     5761 gggccacaac gcgtgctcga tctcgcttgc caggcgcagg cattaatcgc tgaaagcggt
     5821 gcgcaattgc agggcagcgt agaactgcct caccagcgga ttacgccgct cggcactctg
     5881 cgctctacct ggctaagttc gccagaagtc cccgtctggc cgctgcccgc gaagaaaata
     5941 tgtgtagtgg gaattagcgg actgatggat tttcaggcgc accttgcggc agcttcgttg
     6001 cgtgaactcg accttgccgt tgaaaccgca gaaatagagc tgccggaact ggatgtcctg
     6061 cgcaataacg ccaccgagtt ccgcgcggtg aatatcgccc gtttccttga taatgaagaa
     6121 aactggccgc tgttacttga tgcgcttatt cctgtcgcca atacctgcga aatgatcctg
     6181 atgcccgcct gcttcggcct ggctgatgac aaactgtggc gttggttgaa tgaaaaacta
     6241 ccttgttcac tgatgctttt gccaacgctg ccgccttccg tgctgggcat tcgtctgcaa
     6301 aaccagttac agcgccagtt tgtgcgtcag ggtggcgtgt ggatgccggg cgatgaagtg
     6361 aaaaaagtga cctgtaaaaa tggcgtagtg aatgaaatct ggacccgcaa tcacgccgat
     6421 atcccgctgc gtccacgttt cgcggttctc gccagcggca gtttctttag tggcggactg
     6481 gtagcggaac gtaacggcat tcgagagccg attctcggcc ttgatgtgct acaaaccgcc
     6541 acgcggggtg aatggtataa gggagatttt tttgcgccgc aaccgtggca gcagttcggt
     6601 gtaaccactg atcagacgct acgcccgtcg caggcagggc aaaccatcga aaacctgttt
     6661 accatcggtt cggtgctggg cggatttgat cccatcgccc agggatgcgg cggcggtgtt
     6721 tgtgccgtca gtgctttaca tgccgctcaa cagattgccc aacgcgcagg aggccaacaa
     6781 tgaatgacac cagcttcgaa aactgcatta agtgcaccgt ctgcaccacc gcctgcccag
     6841 tgagccgggt gaatcccggt tatccagggc caaaacaagc cgggccggat ggcgagcgtc
     6901 tgcgtttaaa agatggcgca ctgtatgacg aggcactgaa atattgcatc aactgcaaac
     6961 gttgcgaagt cgcctgcccg tccgatgtga agattggcga tatcatccag cgcgcacggg
     7021 cgaaatatga caccacgcgc ccgtcgctgc gcaattttgt tttaagccat accgacctga
     7081 tgggtagcgt ttccacgccg ttcgcaccaa tcgtcaacac cgctacctcg ctgaaaccgg
     7141 tgcggcagtt gcttgatgcg gcgttaaaaa tcgatcatcg ccgcacgcta ccgaaatact
     7201 ccttcggcac gtttcgtcgc tggtatcgca gcgtggcggc tcagcaagcg cagtataaag
     7261 accaggtcgc tttctttcac ggctgcttcg ttaactacaa ccatccgcag ttaggtaaag
     7321 atttaattaa agtgctcaac gcaatgggta ccggtgtaca actgctcagc aaagaaaaat
     7381 gctgcggcgt accgctgatc gccaacggct ttaccgataa agcacgcaaa caggcaatta
     7441 cgaatgtaga gtcgatccgc gaagctgtgg gagtaaaagg cattccggtg attgccacct
     7501 cctcaacctg tacatttgcc ctgcgcgacg aatacccgga agtgctgaat gtcgacaaca
     7561 aaggcttgcg cgatcatatc gaactggcga cccgctggct gtggcgcaag ctggacgaag
     7621 gcaaaacgtt accgctgaaa ccgctgccgc tgaaagtggt ttatcacact ccgtgccata
     7681 tggaaaaaat gggctggacg ctctacaccc tggagctgtt gcgtaacatc ccggggcttg
     7741 agttaacggt gctggattcc cagtgctgcg gtattgcggg tacttacggt ttcaaaaaag
     7801 agaactaccc cacctcacaa gccatcggcg caccactgtt ccgccagata gaagaaagcg
     7861 gcgcagatct ggtggtcacc gactgcgaaa cctgtaaatg gcagattgag atgtccacaa
     7921 gtcttcgctg cgaacatccg attacgctac tggcccaggc gctggcttaa actcctttct
     7981 gatgcccggt aagcatgtgg ttaccgggca tttttgcgta cacgattccg tgcccaatgt
     8041 atgcgttgca acgcagtgaa aattcctctg aaaacgtctc gcaaaggctg aaactggcag
     8101 atgtcaaagg cctgggataa ccgtaatgtc gcgtcatcat aaatatcagg tgacggacaa
     8161 ccatgaccga atcaacaacc tcctccccgc atgatgcagt atttaaaacc tttatgttca
     8221 cacccgaaac cgcacgggat tttctcgaaa tacatttacc agaaccactg cgcaagcttt
     8281 gcaacctgca aaccttacgc ctggaaccca ctagttttat tgaaaaaagt ttacgcgctt
     8341 actactcgga tgttttgtgg tccgtggaaa ccagcgacgg tgacggctat atctactgcg
     8401 tgattgaaca tcaaagctct gcagaaaaga atatggcttt tcggctaatg cgctatgcca
     8461 ctgccgccat gcagcgtcac ctggacaaag gctatgacag agtcccgctg gtggtgccgt
     8521 tgctgtttta tcatggcgaa acctcgccct acccgtactc actcaactgg ctggatgagt
     8581 ttgacgatcc gcaacttgcc cggcagttgt acaccgaagc ttttccgttg gtggatatta
     8641 ccatcgtacc tgacgatgag atcatgcaac atcggcgtat agctctgctt gaactgattc
     8701 aaaagcatat tcgcgaccgc gatttaatcg gtatggtcga caggatcacc acgcttttgg
     8761 ttagaggctt cactaatgac agccagctac aaacactgtt taattatctg ctgcaatgcg
     8821 gcgatacctc ccgtttcacc cgttttattc aggagattgc cgaacgttca ccactacaaa
     8881 aggagagatt aatgactatt gctgaacggc tacggcagga aggacatcaa attggttggc
     8941 aggaaggtaa attagaaggt ttgcaggaag gcatgcatga acaagctatt aaaattgcct
     9001 tgcgcatgct ggaacagggc tttgatcgtg acctggtgct cgcggccacc cagctaagcg
     9061 aagccgatct ggcagcgaat aaccactaat taacacagtc ccacagccga tccccatggg
     9121 cctttgatat caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc
     9181 cagtcgttga tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt
     9241 cgctccccac gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc
     9301 tttacccgca gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg
     9361 cccggcgtta tccgggtagc ccaacgacgc agaaagatcc gcaggtccaa taaacacgcc
     9421 atcaatccct tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac
     9481 ctgcactaac agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca
     9541 gcgcgcagcc cgtgctacac tggccccaac accacgttca ccgtagggag gatagcgcgt
     9601 ggcagacacc acctgacgtg cctgatcggc agtatcgacc atcgggatca gtagagtttg
     9661 cgcgccaata tccaggactt gtttaatcag cggtttactg ccttccaccg gacggatcac
     9721 gggttggctg gcataggggg ctaccgcctg tagctggtga taaagatcct gaatggtgtt
     9781 tggcgcatgc tccccgtcaa tcagcaacca gtcataaccg gaagtagcgg caatttctgc
     9841 catataggca gtcgttgagc ttaaccacag accaatttgc acttcgccct tgcgtaaacg
     9901 ttctttaaag ggatggttaa taatgcgttc atcgtgttcc ttaacctatt taatgatgtg
     9961 ccacgtcggt ctgcgcaacg gtgcgattta cacgcagagt gaaaataatc agcgaaccga
    10021 tgaccgccac cgctgccagc gtcagtaatc ccgccgcatc gctggcaaat aacgtttctg
    10081 ctttcacgcg caggatcggg gcaataaacc cgcccactgc accaagcagg tttacaaaac
    10141 caatccccgc agccagcgcc gtaccggaaa gcagttgtgt cggcatcgtc cagaacaccg
    10201 gctgcacggc aataaacccg atggctgcaa cacacagcgc tacgatcgcc agtactggag
    10261 aaagcagccc ggacagacca ataccaatgc ccgccgccag taatgtcagc gctgcgacat
    10321 tacgccgttc gccggtttta tcggaatagc gcggaataag ccaggttcca aacaaggccg
    10381 caacccacgg aatggcggtg accaccgacg ctgtaaagcc cacttttgtt cccagcaatg
    10441 ccgcaacctg ggtcggcagg aagaaaatta atccgtaaac cgccacctga atggtcaggt
    10501 agataatcgc cagttgccag actcgcccat tacgcagcgc atcgctcagc cgagaagtca
    10561 ctttctgttg ttcttcactt gccagttgat tgataagcaa cgttttttct agtttactca
    10621 gaaaacgtgc ctgctccggt gtgtcatcaa gccaaaagaa tgtgaatacc ccagcgccga
    10681 ctgccaacaa tccttcaatc acaaacatcc agaaccagcc gggatgcccc ataaatccat
    10741 gcatctccag cagcgcgccg gaaagcggtg atcccagtgt taacgccagc ggtgcgccca
    10801 tatagaacag ccccataatg ctggcgcgat tacgctgcgg aaaccattgc gaggtgagat
    10861 aaatcatacc agggaaaaat ccggcctccg cagcaccaag cagagtgcga actatcagaa
    10921 atttcgcttc agtatcggcc catgccatgg ctgccgaaag aaatccccac agcagtgttg
    10981 tcgtaccaat ccaggttctg gcccccagtt tgcgcatcaa aagattcgcc ggaacaccca
    11041 gaaacgcata taccacaaag aaaatgcctg ctcccagcgc ataagcttca ttactcaacc
    11101 cggtatcaat ctggtaggtc tgtttggcaa aaccgatatt cgaacggtcg agaaacgcca
    11161 gcacatacag cgccaacata aacggaatta aacgcacacg gtttttcttc accacggcgt
    11221 caagcaaagc ggtgctcata aaaagctcct tagaatatgt gggcgttacc gttgtcacag
    11281 caacgcccgg agtgattagt ggctgtaggg gcgtttcaaa ttgcagtcac gattgagttc
    11341 gacgccaaaa ccgggtttat caagcactga tttatgaata cgaccattca ccggaaccgg
    11401 ctcattgagc agaatcgggt caaactgcgg acgcatcgtt gaacaatccg gactagtcat
    11461 caggaattcg ctgaatggcg tattagtgaa ggtgatcacc gcatggtggg agtaaacaga
    11521 cgaaccgtgc ggcaccacca gttgccccct ggatttggcg attgcggcaa tttccaccag
    11581 cgtggttaag ccaccgcacc agccaacatc cggctgcata atgtcgatac cggtttctga
    11641 aagcgtacga aaagattgca gtgtgccgtg gtgctcaccg ctggtgacca tcattccgac
    11701 tggcgcgttg cgtttcagtt cgcgataacc ttcatactgc tgtggcggca ggcactcttc
    11761 gatccatttc aggttatagg gcgcgcaagc gtgggccagt ttggtcgcat agttcacgtc
    11821 ctgactcatc cagcagtcga gcattaacca gaaatcctca ccgcattttt cacgcatatc
    11881 cgcgaccata gcggcatctt tgcggatccc cgcatcgcca tcatgtggcc cccagtgcgt
    11941 cggcattttg ccaccgataa agcccatctc ttttgccaga tccggacgcg cacctgtggc
    12001 gtagaactga atctcatcac gaacagcgcc gcctaaaagt ttataaaccg gaagcccgac
    12061 cactttgccg aacagatccc acagagccag atcgacacag gaaatcgtgt tcatcaccag
    12121 gccaccagag ccggagtaat acagggtggc attgagcatt tgatcatgga tcagtttgat
    12181 atcactgaca catttaccct caatgaaacg gttaaggtgt ttttcgacga taaaacagcc
    12241 catttcaccg gctgtcgaaa cagcgaatcc ggtctggccg ttttctgctt cgacttcaac
    12301 aaccaacgtg ccaagaacgt taatgccaaa tgactggcgt aactgctcgt aatcgcggta
    12361 tttactcatc ggggtggcaa tatgatcgtc tatccagtga ttcgccccct ggtcgtgata
    12421 atcaccgccg ccagcgcctt tttctgctgt cgcaccgcca gtaaaccagg cgcgaacctg
    12481 tttaattttt ggtagggtca tgatgttctc cattgttatg aggcttgtaa gtcaaaggga
    12541 cttttccatc ccaacagacg tgaaatatcc ctggcgcagg caatggcctt gcccgccaga
    12601 taatcacggt attcttcatt gatttgtaag cgggtaccga ccaccgagat cgcagcggta
    12661 agctcgttat tggcgttaaa caccggcgca gcgacacaac ggacatcggc gtaatcttcg
    12721 ccgttgtcat agctccagcc ctgacggcga atacgcgcca gttcttcgtg aagttgctgt
    12781 ggatgagtaa tcgttgtggg tgtcgcctgc tcccagacca gcccttcgat aatactttgc
    12841 tgtacagttg caggttgcca ggcaagcagg catttaccta ttccggagcg ataaagcgaa
    12901 aggctttttc cttcatgtga acgcacgctg atagtggctg atgactccac tttcaaaatg
    12961 taataagcgc tgccgttatc aataatcccc aggtgacata acagcccggt ggtatccatc
    13021 agttgcgtca aacgcggtcg agccagttcc cggagatcca tcttgcttaa cgcatgaccg
    13081 gaaagctcca ccagcttggt ccacaaacaa aaattttcct ggtgatcaag gctaagaaaa
    13141 cgctgacgcc tgagttcatt aagcagcaaa taggccgtgc ttttagggat ccccaatgtg
    13201 tcaatgatcg tcgcagcatt acaggggcca atgcgggcaa tcaaattgag aatatcaata
    13261 gcccgtgtca gggcgggaac tttgcttgat tccaacatac tggactccag tcttagatac
    13321 tggaatcagt ctcgttgctc agggggagta aaattgtgaa gcgcatcaaa tcccgctcct
    13381 cgtccaacgg actggagaaa ttacgcacaa atttgtgaat cagcgcacgg attttttctt
    13441 attgagtaca acaggttgga caagttttgc cggaggggga atgtgccggt gcagtaaacc
    13501 ggcacagagt ggcggaatca gacggataag gtcatggact caacaacctc aatccagcca
    13561 tgctcactgg caatgtcctg gccgtttaac caacggcgta gcatattcag tgccatcatt
    13621 gcgcacactt cctgacggat agccaggcta tagcgtgtag tgctgaaacg cacacgcaga
    13681 gctaaagtgc catctggcgt ggctagcgca aagttgagat gctcgttctc gaaacccgaa
    13741 acagccagtg ccagcccggc aaaatggttg gctcgccgtt ctgtaatcca gtgcgcagtt
    13801 tgcgccaggg tttcttcctg tgaaggaacc acttcacacg ccagcagtgg agcacctgcg
    13861 cgagaaagtt gcaaagccaa taaaccaccg gtgaattgct cgctcaacgt caggctgaac
    13921 tggcggtttt gcaattcgcg actgatctgc gcgggcagtc cttcggtgcc ttcgaaaatc
    13981 acgctctgtc cggcaacacg tttaacgtcc agccacagtt tttccatcgc ctgttgctcg
    14041 cttgccggtc cggtgagttt cagttcgatg atcggcattg aggagcgata gcccattgtt
    14101 acgcccggcg gcagttgtag agtgtccagg ctttgtgcca gatcgctttc cgaacgacca
    14161 aaggtagtta aacgcagaca aaccggcggc tgtggtaaag aaaaacgctc gcgcagacgc
    14221 ggcaggattt cgtgctcgac catcacctta aattctgacg gtacgccggg agtaaagaac
    14281 atcaggcaac gattaagctg cacggcaaaa ccacaggcgg tgcctaccgg gttattgata
    14341 aattcagcac tggcaggcag ctccgcttgt ttacggttgc tcggtgccat tactcgtcca
    14401 cgttcgtgga aatagcgttc catctctttg agccaggctt catgcagcac caggccttca
    14461 ccttttgctg tcgcagcggc gagtgcgctt aaatcatcgc tggtcggtcc cagcccgccg
    14521 ttaacgatca gcacatcggc gtgctgacta cgttcgcgaa gaatggtgac taagtcatca
    14581 aggttatccc ccaccgtatt gcggcgagat aatggcaacc cctgatgaaa gaaaaaatcg
    14641 gccagccagg cagcgttagt gtcaacgatt tgcccgtgta acacttcatc cccggtggat
    14701 agcatttcca cttttaacat ggttatctcc tgcttttgtt atcgaatcac tataacgcaa
    14761 agggagataa ggcgtagtaa gaaactgaat ggcgcgacag aatgccgcgc cggaagggat
    14821 cagaaactgg cgttaacccc aacatacggg ccgtcagcca cggcgttgtc gcggttaccg
    14881 tctttacccg acagattcag gtagcgataa cccgcctcaa tactgactgg acgcataatg
    14941 gtgtaacgcg cgccagcatt cgcttcctca taacttttaa taccgctgga gagtgaatcc
    15001 ggagagtagt aatactcgcc aaacaaacgg aagctgttgc caattttcca ctgcaaacca
    15061 cctcctaccg ctgcggcata gccttcatcg ccgtaattcg ggttggtgta aacgcctttt
    15121 ccgccaacgg tcgccattaa cggcccgaga ggcagattca accccagccc cacgcccgcg
    15181 acgtcaccgt cgtcgtcgtt atgtgtccag ttaccgctta aagccaggcc cgtcgattca
    15241 gtcccaaaac cgacaccaat attggtgtat tctttacccg cctggccgct gatgctcatt
    15301 gcattgaccg atgcagaaac caacagcatt ccggccagac ctgctagcgc aatttttttc
    15361 attatcccct ttcccacaag caattaatca aaattcccaa agctgcggga ttgtactgcc
    15421 tggcgcagag gattcaatga gcgaaaaaag ggatctagtc acttattgta actaaatact
    15481 tcttaaataa ttaaacgccg aaaaaataaa cacagtttac cgatacacac aaaaataata
    15541 ataagtgcag ttatcatcgc aaaagagatt actgtcactt tcacatattt ctgagttacc
    15601 gtattctcat ggtttctttt tatttataag gaatacccct gtgcgacaac ggactattgt
    15661 atgccctttg attcaaaatg atggtgctta tttgctgtgt aaaatggccg acgatcgcgg
    15721 cgttttcccc ggtcaatggg cgctttcggg tggcggcgtg gagcccggcg aacgaattga
    15781 agaggctcta cgccgcgaaa ttcgcgaaga actgggagaa cagctgcttt tgacagaaat
    15841 cacgccctgg accttcagcg atgatattcg caccaagacg tatgcagatg gtcgcaagga
    15901 agaaatttat atgatttacc tgatttttga ttgcgtttct gccaaccgag aagtgaaaat
    15961 aaacgaagag tttcaggact acgcgtgggt aaaacctgaa gatctggcgc actatgattt
    16021 gaatgtcgcc acccgaaaaa cgttacgttt gaaaggtctt ctgtaacaat gatgacagcc
    16081 attactggct gtcagattga aaatttagtg attaacgaat tccccatcca gataaacttt
    16141 gcctttttcc acatgcatga ctaaaccatc cagataatca ggtttaaatg tcgcatcacg
    16201 cttatcttta gcaatatatg tcaggcaatg attatgggtg aaaataacga tatttttatc
    16261 aggcgctttg ctttgtaagt ccttaatcgc actataaatc tcattaccgc actgaagaag
    16321 tcgtttatct accgtcaatt ttttacccgc tgaaaaccag gtcgcggact gaatggtccg
    16381 gacggtatta ctggaataaa gatcgaaatc agggatatca gcactaaaag cgttgcccag
    16441 ttcacgggca tcctgggtac ctttaaccgt aatacctgtt ttatctgaca agcattgatt
    16501 ggttgaacga tcgcaacgtt cagcatggcg aaacaaaacg acaaccgggt gctgctgtgc
    16561 cagtctgccc agcgttttat tgtcgatacg tggcaaacca ttgctactcc aggcggcatg
    16621 agtacccagt ccggcaattg cagcgagcgc cagtaaaatg atgaaatatt tttttgactt
    16681 caatgatgag cggcaaaaag ctaacacgta aactccacct atagacaagc gcaaccagac
    16741 aattaccgtg aaattgagcg acatttctgg cgataattag cagttggtgt aatattaaaa
    16801 atcctacgat gctggcaaaa tgcctcaaaa ttttgccaaa tgcaaagcct aaataagaaa
    16861 aaatataaaa atttcaatat ttacgtctaa tattagtttc ttaaggttaa gttaatattc
    16921 ta
//
LOCUS       DAZFBR010000102        16692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFBR010000102 DAZFBR010000000
VERSION     DAZFBR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.102"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..197)
                     /locus_tag="ACHJZ2_003691"
     CDS             complement(<1..197)
                     /locus_tag="ACHJZ2_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO4569726.1"
                     /translation="MILSTRISLGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLT"
     gene            complement(344..1627)
                     /gene="yeaH"
                     /locus_tag="ACHJZ2_003692"
     CDS             complement(344..1627)
                     /gene="yeaH"
                     /locus_tag="ACHJZ2_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO4569727.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1740..3674)
                     /gene="yeaG"
                     /locus_tag="ACHJZ2_003693"
     CDS             complement(1740..3674)
                     /gene="yeaG"
                     /locus_tag="ACHJZ2_003693"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO4569728.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4110..4856
                     /gene="mipA"
                     /locus_tag="ACHJZ2_003694"
     CDS             4110..4856
                     /gene="mipA"
                     /locus_tag="ACHJZ2_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO4569729.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            4946..5800
                     /gene="yeaE"
                     /locus_tag="ACHJZ2_003695"
     CDS             4946..5800
                     /gene="yeaE"
                     /locus_tag="ACHJZ2_003695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO4569730.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5851..6735)
                     /gene="yeaD"
                     /locus_tag="ACHJZ2_003696"
     CDS             complement(5851..6735)
                     /gene="yeaD"
                     /locus_tag="ACHJZ2_003696"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO4569731.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6819..7814)
                     /gene="gapA"
                     /locus_tag="ACHJZ2_003697"
     CDS             complement(6819..7814)
                     /gene="gapA"
                     /locus_tag="ACHJZ2_003697"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4569732.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            8156..8569
                     /gene="msrB"
                     /locus_tag="ACHJZ2_003698"
     CDS             8156..8569
                     /gene="msrB"
                     /locus_tag="ACHJZ2_003698"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO4569733.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8611..8883
                     /gene="yeaC"
                     /locus_tag="ACHJZ2_003699"
     CDS             8611..8883
                     /gene="yeaC"
                     /locus_tag="ACHJZ2_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO4569734.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9253..10329
                     /gene="ydjL"
                     /locus_tag="ACHJZ2_003700"
     CDS             9253..10329
                     /gene="ydjL"
                     /locus_tag="ACHJZ2_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4569735.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10356..11735
                     /gene="ydjK"
                     /locus_tag="ACHJZ2_003701"
     CDS             10356..11735
                     /gene="ydjK"
                     /locus_tag="ACHJZ2_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4569736.1"
                     /translation="MDQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGTPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11752..12795
                     /gene="ydjJ"
                     /locus_tag="ACHJZ2_003702"
     CDS             11752..12795
                     /gene="ydjJ"
                     /locus_tag="ACHJZ2_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO4569737.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12816..13652
                     /gene="ydjI"
                     /locus_tag="ACHJZ2_003703"
     CDS             12816..13652
                     /gene="ydjI"
                     /locus_tag="ACHJZ2_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO4569738.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13657..14589
                     /gene="ydjH"
                     /locus_tag="ACHJZ2_003704"
     CDS             13657..14589
                     /gene="ydjH"
                     /locus_tag="ACHJZ2_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4569739.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14599..15579
                     /gene="ydjG"
                     /locus_tag="ACHJZ2_003705"
     CDS             14599..15579
                     /gene="ydjG"
                     /locus_tag="ACHJZ2_003705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO4569740.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            15716..16474
                     /gene="ydjF"
                     /locus_tag="ACHJZ2_003706"
     CDS             15716..16474
                     /gene="ydjF"
                     /locus_tag="ACHJZ2_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4569741.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16592..>16692
                     /locus_tag="ACHJZ2_003707"
     CDS             16592..>16692
                     /locus_tag="ACHJZ2_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569742.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLT"
BASE COUNT         4358 a         3736 c         4201 g         4397 t
ORIGIN      
        1 gtcagcaata aactgaaaat aaaaaagctt tgagctttta atttttgtgt acccatagaa
       61 accataaaaa ggagagcaat gtgagtgctg gattttattg atggcagaga tatatagttt
      121 tgaccaggat caattttcgt taacgataaa aaatatttaa agaataaccc cagggaaata
      181 cgtgttgaca gaataatttt tccgaatgat tgttttctgg aactaaattg tgtaaaggtt
      241 atgctgtcat atttaaagcg attgtaagct aatgtatgta ataaatgaga taacttacag
      301 tgagctaaaa atcagccagg cgataatgac ccggctgata gttttagtct ttagctgttg
      361 cattttgctt atgaaacagt tcacggaaca ccggataaat atcatcctgg tcgcggatat
      421 gctgcatcgc aaagttgtcg aaagtagatt gcagatgctc atattctcgc cacagtgtct
      481 gatgcgcgcg acgggtaatt tcgatatagc tgtaataacg aacaacgggt aataattttt
      541 tcgccagaat ttcatggcaa agcggagagt catcggccca gttatcgccg tccgatgctt
      601 gtgcagcgta aatattccac tgtgccgggt tataacgctc tttcactacc tcatccatca
      661 gtttcagggc gctggaaaca atggtgccgc cagtttcctg cgagtagaaa aactcatgtt
      721 catcgacttc tttcgcctgg gtatgatggc ggatgtatac gacttccacg ttcttatacg
      781 ttctgctgag gaacagatac agcagaatat aaaaacgctt agccatatct ttagtggatt
      841 gatccattga accggaaacg tccatcaggc aaaacatcac tgcctggctg gagggatcgg
      901 gccgcttctc gtagttcttg taacgtaaat cgaaggtgtc aataaaaggg acgcgttcaa
      961 ttttggcacg taattctgca atttctttgc gcagacgttc ctcttccagc agctgcgcag
     1021 gttcactgtt gctgatgatg gccaaattct cttccagtgc atgaagttcc cgccgcttgc
     1081 ctgccgtcat ggctgtgcgt cgcgccagtg agttctgcaa ggaacgcaca acgctgatat
     1141 tggccggaac gccgttagcg gtataacccg cccgatgcgt tttatattcg gtcagctggc
     1201 gttgttggtt ttgtttcaga ttcggtaagg ccaaatcttc aaagagcaga tcaagatact
     1261 catctttcga aatctgaaag acaaattcat cctgaccttc accatcctgg ctggcctggc
     1321 cctgaccact gccggaacct ccgccgccac cctggggacg ttcaattcgg tcgttctgaa
     1381 cgaaatggtc attgcccgga tgcacgcggt ggcgcagacc gccacgcccc tgatgaaaca
     1441 ttggttcgct aatatcttcc gtgggaatgg atacggactc gccgctgtcg acgtcagtca
     1501 ccgaacgctt attaatggcc tcggagatcg actgtttaat ttgcgcttta taacggcgta
     1561 aaaaacgctg gcgattcacc atgcttttgt ttttgccgtt cagacgccgg tcaataaacc
     1621 aggtcatatg ccccccgtac tacatttgcc aactgtatgc tacgcctgtg ttgccatttc
     1681 tctggcccac ggttcgctgt cgttgtaggc ccggtagcat gtgtaccggg ccagggttgt
     1741 taagacgatt tacgtacgcg caaataccat tcgcacagta aacgcacctg tttacgggtg
     1801 tagccttttt ccatcatacg gtcgacaaag tcgtcgtgtt tcttctgctc gtcggttgac
     1861 gttttggcgt taaacgagat aaccggcaac agctcctcgg tattggagaa cattttcttc
     1921 tcgataaccg tgcgcagttt ttcatagctg gtccagttcg gattgcgtcc gctgttattg
     1981 gctctggcgc gcagtacgaa gttaacaatc tcgttgcgga aatcttttgg attactgatc
     2041 cccgccggtt tctcgatttt ctccagctcg gcgttaagag actcgcggtc aaacagctgc
     2101 ccggtatccg gatcgcgata ctcctgatcc tgaatccaga aatccgcgta ggtaacataa
     2161 cggtcgaaaa tgttttgccc atattcggaa taggattcaa ggtaggccgt ctggatctct
     2221 ttgccaataa actcggcata tttcgggatc agataacctt tcaggaactc cagatagcgt
     2281 tctgcctgct cttgtgggaa ctgctcgcgc tcgatctgct gttccaggac gtagaacaga
     2341 tggaccgggt ttgccgccac ttctacatga tcgaagttga acacgcggga gaggatctta
     2401 aacgcaaaac gcgtcgacag accgttcata ccttcatcga caccggcgta gtcacgatat
     2461 tcctgatacg acttggcttt gggatcggtg tctttcagac tttcgccatc ataaacccgc
     2521 atctttgaat aaatgctgga gttttctggc tctttcaggc gcgaaagaat ggaaaaacgt
     2581 gacagtgttt cgagagtgcc aggggcgcat ggggcgtgag tcaattcact gtgattaagc
     2641 aatttctcgt agattttgat ctcttcggaa atgcgcaagc aatacggcac cttcacgatg
     2701 taaacacgat cgaggaaggc ttcgttgttt ttgttattac ggaaagtgac ccattcggac
     2761 tcgttcgagt gggcaagaat aatcccgttg aacggcaggg cggagatacc ttccgtcccg
     2821 ttgtagttac cttcctgagt ggcggttaac aagggatgca gcacttttat cggtgcttta
     2881 aacatctcga caaattccat gatcccttgg ttggcgcggc acagcgcacc ggaatagccg
     2941 taggcgtccg ggtcattctg cgcgtaatgt tcgagtttac gaatatcgac tttcccaacc
     3001 agcgcggaga tgtcctggtt gttctcatca ccgggttccg ttttggcgat agcaatttgt
     3061 tgcagaattg acggccagac cttcactacc cggaacttag tgatatcgcc accaaattca
     3121 tgcaggcgtt ttgccgccca cggcgacatg atggtgccga gataacggcg aggaatgcca
     3181 tactcttttt ccagaatctg cgcatcttcc tgcggattga aaagacagaa cggatgatcg
     3241 ttgaccgggc tacgctcacc gttggcgctc aatacataaa tcggtacgag ctgcattaat
     3301 gatttcagtc gctcagcaag cgatgattta cccccaccca caggccccag cagatacagg
     3361 atttgtttct tctcttccag cccctgagcc gcgtgtttca gataagagac aatctgttca
     3421 atcgcgtctt ccatgccgta aaactcttca aacgccggat aacgtgcaat gacccggtta
     3481 gaaaagagtc gagaaagtct gggttcctgg gctgtatcga ccatgacagg ctcaccgata
     3541 gccatcaata gccgctcagc cgcgttggca taagcactgc gatcttgccg acaagtggta
     3601 agaaactcct gcagtgtgaa ctcttcgtcc ttggcagctt catatcgctg gcgatagtga
     3661 tcgaatatat tcatggtatg ccgtcctttc gttttttagc acaggttaag agccgttcat
     3721 atgaatagta gaggctcccg gaagaggtaa actgaacgac gtctcttgct aaaacacagc
     3781 aactctcatg ccattttatc gacgatgaga acaacgtgcc cggtgataca ccttcaaaat
     3841 taagcgtaga tggcaaatgg aaaacttgcc tacacggctt tcttaatttc aatggcatat
     3901 caacaactca tccataaatt ttgcataatt aatgtaaaga ccaggctcgc cagtaacgct
     3961 aaattcattt ggctgtaagc gcggtgtcat ccgcgtcagg aaaattaaac agttacttta
     4021 aaaaatgaaa acgtaaaaag gttgggtttc gatgtattga cgggtaaact ttgtcgcccg
     4081 ctaaacattt gtttaaggaa tgattaattg tgaccaaact caaacttctg gcacttggag
     4141 tgcttatcgc aacgtctgca ggcgtagcgc acgctgaagg taaattttcc ctgggcgcag
     4201 gcgtaggtgt cgttgaacac ccatataaag attacgatac cgatgtttac ccagtaccgg
     4261 taatcaacta tgaaggcgat aacttctggt tccgtggctt aggtggtggt tactacctgt
     4321 ggaatgacgc aacggataaa ctttcaatta ccgcttactg gtcgccgctt tacttcaaag
     4381 ctaaagacag tggcgatcac caaatgcgtc acctggatga ccgtaagagc accatgatgg
     4441 ctggtctgtc ttatgctcac tttacccagt acggttacct gcgtaccacc ctggctggcg
     4501 ataccctgga taacagcaac ggcatcgtct gggatatggc ctggttgtat cgttacacca
     4561 acggtggcct gaccgtgact ccgggtattg gtgtgcagtg gaacagcgaa aaccagaacg
     4621 aatactatta tggcgtatcg cgcaaagagt ccgctcgcag cggtctgcgt ggctataact
     4681 cgaacgacag ctggagccct tacctggagc tgagcgccag ctacaacttc ctcggcgact
     4741 ggagtgttta cggtaccgcg cgctacaccc gtctgtctga tgaagttact gacagcccga
     4801 ttgtggataa atcctggact ggcctgattt ctaccgggat cacctacaaa ttctgataat
     4861 gcatctttcc agggcatttt gtgcataaaa acagggcgtc acgccctgtt ttgcattacg
     4921 gtggtgcaat acctgggagg aaacaatgca acaaaaaatg attcaattta gtggcgatgt
     4981 ctcactgcca gccatagggc agggaacatg gtatatgggc gaagatgcca gtcagcgcaa
     5041 aacagaagtt gctgcactac gcgcgggcat tgaactcggt ttaaccctca ttgataccgc
     5101 cgaaatgtat gccgatggcg gtgccgaaaa ggtggttggg gaagcattaa ccggtctgcg
     5161 agagaaggtc tttctcgtct ctaaagtcta tccgtggaat gctggcgggc aaaaagcgat
     5221 aaatgcatgc gaagccagtt tacgccgtct caatactgat tatctcgatc tctacttatt
     5281 acactggtct ggcagtttcg cttttgaaga gactgttgca gtgatggaaa aattgatcgc
     5341 ccagggaaaa atccgccgct ggggcgtttc gaaccttgat tatgctgata tgcaggaact
     5401 ctggcagctg ccggggggaa atcagtgtgc cactaatcag gtgctttacc atctcggttc
     5461 acgagggatt gagtacgatc tactcccctg gtgccagcaa cagcagatgc cggtgatggc
     5521 ttacagtccg ttagcccagg ccgggcggtt gcgcaatgga ctgttaaaaa acgcggtagt
     5581 caacgaaatt gcacatgctc acaatatcag cgcggcgcaa gtattgttgg cgtgggtaat
     5641 cagccatcag ggtgtgatgg cgattccaaa agcggccacg attgcccatg tccaacagaa
     5701 tgcggctgtg cttgaggtcg aactttcgtc agcggaatta gccatgctgg acaaagcgta
     5761 tccggcacca aaaggaaaaa ctgcgctgga tatggtgtga tgttgatacg tgccggatgc
     5821 atcgcacccg gcacacaaca ttgacgcaaa ttaacgtttg gcaacgcgaa tggattgcgc
     5881 cagatgtgca ggtttttctt tggtcacttt ttgcatttct gaggcgtaag ctgtttctac
     5941 acaaacaaag gtcttgtagc catcgtccgg catgtcgccc atgctaactg aaagcgccgg
     6001 tcccgggttc cagcctacaa cgttcagatg atgctggtgg cctacggcga taatacgatt
     6061 cagcgcttca tcattaatca cgctgcaatc ttgcggattc agatacacgc gatcggtacg
     6121 gtcagggaag gtctgaatac cgtcggtcag tacatcttct ttcgcgtcat ttactttatc
     6181 aatgaagcga tcgcccagcc cactgacgct taccttagcg atatcaccca cgttaaagta
     6241 ggtatgcagg gcagaggtgg tttcaaattc gccgtgtgct tcaagatcga tttcacagtt
     6301 tttatccacg cggaaatgcg ctaacagagt aaagtcgtgc agccagaatt ttttcgtctc
     6361 ttcgctttgt gtcaattcaa aagtcagcgc tacgccatta gcatcttcac gatgtgattt
     6421 cagcgtccac ggcaggttgc gggcaaaacc gtgcgcaggc aggccttgtt gtgccgccgg
     6481 accaaaccac ggccagcaaa ccggtacgcc accgcgaata gcaacgccat ttttgaacgg
     6541 tgtgttgttg ctcaaccaca gaacttcttc ttcacctgca ggtttccacg agagaaggtg
     6601 tgcgccctgt aatgcaaaag aggcttttac ctggggatga tcgactacaa tgaggtccag
     6661 ttcatccagt ttacgacggg agaggacagg ggagatttgt tcgatgaccg gaagggcaaa
     6721 aattttctta atcatgacgc agtcctttaa cttcatttta tcaggtaaaa aaaagagcga
     6781 ccgaagtcgc tctttttaga tcacagtgtc atctcaactt atttagagat gtgagcgatc
     6841 aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg
     6901 aagttgtcgt tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg
     6961 ttgaaatcgg tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct
     7021 tcagcagcag ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga
     7081 acggtcaggt caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt
     7141 ttgccattca gttctggcag tactttacct acagctttag cagcaccggt agaggacggg
     7201 atgatgttct gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg
     7261 gttttctgag tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag
     7321 ttatcgttga taactttagc cagcggagcc aggcagttgg tggtgcagga agcgttggaa
     7381 acgatgtcct ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg
     7441 tctttagacg gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca
     7501 gtttcgtcag tcaggaacag accagttgct tcagcgacaa cgtcaacacc aacttcgtcc
     7561 catttcaggt tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc
     7621 agatgaccgt ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc
     7681 agcatgtatg ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca
     7741 gaacgtttct gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct
     7801 actttgatag tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa
     7861 ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc
     7921 tgtgcctaag cattacgcga ctgactcgcc tcactcttcc tttgggcttg agaccacatg
     7981 gggtcggcgc cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt
     8041 taggtgcagc catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat
     8101 gttttgttag aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agcaaatggc
     8161 taataaacct tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac
     8221 gcagaatcat gggacagagc cgccatttac gggtcgttta ctgcataaca agcgtgacgg
     8281 cgtatatcac tgtttgatct gcgatgcccc gctgtttcat tcccaaacca agtatgattc
     8341 cggctgtggc tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa
     8401 agacttgtca catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct
     8461 ggggcatgtc ttccctgacg ggccgcagcc aacgggcgaa cgttattgtg ttaactctgc
     8521 ttctttacgc ttcactgatg gtgaaaacgg cgaagaaatc aacggttgaa aaaacgattc
     8581 agcaacttat tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg
     8641 acgcctgagg tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcctgatggc
     8701 gttgcgttga ccgaggaaca aaaagagaac tgcctgcaac tggtgatgct gtggcaagcc
     8761 cgcaataata ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag
     8821 agcaaacagc agttaaaaga agattttggt atctcagcaa agcctattgc catgtttaag
     8881 taagtggttc atcccctttt tgccgcggct atgaagggga tagccaacaa tgaccccacg
     8941 actcatctca attatctcgc catgacctgg ctaagtccca gtccgatctc ttgccgtcgc
     9001 gttcacgcct gaatgacttc tcctgaactt aagtctcaga cctatttggc cggtaatccc
     9061 tctcaaattt gctccttttg cgttccgcct tacatcaacc tgtaacagaa ccagccattt
     9121 tctgcgtaat tgtgatctga gttgtaaaac gcagattatt atctgtgttt tacatgttta
     9181 atgttgacct caaaaatcat gtgggtaagt atgatgacca tctaagcaaa acaaaacaca
     9241 gaggatgacg caatgaaagc actggctcgg tttggcaaag cctttggcgg ctacaagatg
     9301 atcgatgtgc cacaacccat ttgtggcccg gaagatgtag tgattgaaat taaagccgcg
     9361 gcaatctgcg gcgcagacat gaagcactac aatgtcgata gcggttctga tgagtttaac
     9421 tctatccgcg gccatgagtt cgcaggttgt attgcgcagg ttggtgaaaa agtcaaagac
     9481 tggaaagtgg ggcaacgcgt cgtgtcggat aacagcggtc acgtctgcgg cgtttgtccg
     9541 gcctgtgaac aaggtgattt tctgtgttgt acagaaaaag taaaccttgg tctggataac
     9601 aatacctggg gcggtggttt ttccaaatat tgtctggttc ctggtgaaat tctcaaaatt
     9661 catcgtcatg cgttgtggga aatccctgat ggtgttgatt atgaggacgc agccgtactt
     9721 gaccctatct gtaatgccta caaatccatc gcgcagcaat cgaaattcct ccctggtcag
     9781 gatgtggtcg tcatcggcac tggcccactc gggctgttct ccgtacaaat ggcgcgaatt
     9841 atgggggcgg taaatatcgt cgtcgttggt ctgcaagaag atgtggcggt ccgcttcccg
     9901 gttgcaaaag aactgggtgc gacggcagta gtaaatggtt ctaccgaaga tgtggtggcg
     9961 cgctgccagc aaatttgtgg caaagacaat ctgggactgg tgattgaatg ctccggtgcc
    10021 aatatcgcac tgaaacaagc catcgaaatg ctccgtccga acggggaagt ggtacgcgtt
    10081 ggaatgggct tcaaacctct tgatttctcg attaatgaca ttaccgcctg gaacaaaagc
    10141 atcattgggc atatggccta tgactccacc tcatggcgta acgctatcag gctattagcc
    10201 agcggcgcta tcaaagtcaa accgatgatc acgcatcgta tcggcctgtc gcaatggcgc
    10261 gaagggtttg atgcgatggt cgataaaacc gcaatcaaag tgatcatgac ttacgacttt
    10321 gatgaataac cactctcctg aaggaacgtt atcgcatgga tcagataaca aaaccgcatt
    10381 gtggtgcacg gctggatcgc ttaccggatt gccgctggca ttcatcaatg tttgccatcg
    10441 tcgcgtttgg cttgcttgtc tgctggagta atgccgttgg tggcttgatc ctcgcgcagc
    10501 tgaaagcgtt gggctggaca gataattcca ccacagccac attctcagca atcacgaccg
    10561 ccggaatgtt tctcggtgcg ctggtgggcg gcatcattgg cgacaaaacc ggtcgcagaa
    10621 atgcgttcat cctctatgag gccattcata ttgcctcgat ggttgtcggt gctttctcac
    10681 cgaatatgga tttccttatt gcctgccgtt ttgtgatggg agttgggctg ggagctttac
    10741 tggttacgct gtttgctggt ttcaccgaat atatgcccgg tagaaatcgt ggaacgtggt
    10801 caagtcgggt ttcctttatt ggcaactggt catatccgct ctgttcattg atagcgatgg
    10861 gactcacgcc gctgattagt gcagagtgga actggcgggt acaactgctt atccctgcaa
    10921 tattgtcgct tatcgctacg gcgctggcct ggcgctactt tcctgaatcc ccgcgctggc
    10981 tggaatcgcg cggacggtat caggaagccg agaaagtcat gcggagtata gaagaaggcg
    11041 tcatacgcca tacaggtaaa cctttgccgc ccgtggttat tgctgatgac ggtaaagcac
    11101 cacaagcggt gccatattca gccttactga caggagtatt actgaaacgc gtgatattag
    11161 gctcttgtgt gctgattgcc atgaacgttg tgcagtacac actaattaac tggttgccaa
    11221 cgatattcat gacccagggg attaatttaa aagactcgat tgttttaaat accatgagta
    11281 tgtttggcac gccatttggc atttttattg ccatgctggt gatggataaa attccgcgta
    11341 aaacaatggg tgtggggcta ttaatcctga ttgcggtgct cggatatatc tattcactgc
    11401 aaaccagcat gttgctcata acgttgattg gtttcttcct gattactttc gtctatatgt
    11461 acgtttgcta tgcctcggca gtgtatgtcc ctgagatctg gccgacagag gccaaactcc
    11521 gtggctccgg tctggcgaat gcggtagggc gaatcagtgg tattgccgca ccttatgccg
    11581 ttgcagtgct gctcagtagt tatggcgtta cgggagtctt tattcttctg ggggcggttt
    11641 caattattgt cgcaattgct atcgccacca ttggaatcga aaccaaaggt gtctccgttg
    11701 aaagtttaag tattgatgca gtagccaata aataatacgg gtggattcaa aatgaaaaat
    11761 tcaaaagcaa tattgcaggt gccgggcaca atgaaaatta tttcagcaga aataccagtg
    11821 cctaaagaag atgaagtttt gattaaagta gaatatgtcg gtatttgtgg ttcagatgta
    11881 catggttttg aatcaggtcc gtttattccg cctaaagacc caaatcaaga aattggcctg
    11941 ggtcatgaat gcgccgggac ggttgtggct gtgggaagcc gtgtgcgcaa atttaaaccg
    12001 ggggatcggg taaatatcga acctggcgtt ccttgcggtc actgtcgtta ctgtctggaa
    12061 ggcaaatata acatctgccc ggacgttgat tttatggcga cacaacccaa ctaccgcggc
    12121 gcattaacgc actatctgtg tcatccggag agctttactt acaaactgcc cgacaatatg
    12181 gacacgatgg aagggacgct ggtggagcct gccgcagtcg ggatgcatgc cgcgatgctg
    12241 gcagatgtta aaccgggtaa gaagataatt attctgggag caggttgtat tggtttgatg
    12301 acgttgcaag cgtgcaaatg cctgggagca acggaaattg ccgtcgttga tgtgctggaa
    12361 aaacgtctgg caatggcgga acagcttggt gcgacagtgg ttattaacgg cgcaaaagaa
    12421 gacactattg cacgctgtca gcaatttacc gaagacatgg gcgcagatat tgttttcgaa
    12481 acagcgggtt ctgcggtcac cgttaaacag gcaccttatc tggtaatgcg tggcggtaaa
    12541 attatgattg ttggtactgt acccggcgct tcggcaatca atttcctcaa aatcaatcgc
    12601 gaagtcacta tccagacggt attccgctat gccaatcgtt atccggtcac gattgaagca
    12661 atttcttcag ggcgattcga tgtgaaatcg atggtgacgc atatttacga ttatcgggat
    12721 gtacaacagg catttgaaga gtcagttaac aacaaacgcg acattattaa aggcgttatt
    12781 aagattagcg attaattaat acgggagaac agaacatgct cgcagatatc agatattggg
    12841 aaaacgatgc cacaaataaa cgttatgcta ttgcccattt taatgtatgg aatgcagaaa
    12901 tgttgatggg cgttatcgac gcagccgaag aagcgaaatc accggttatt atttcttttg
    12961 gcacaggttt tgtcggcaac acctcatttg aagatttctc tcacatgatg gtatctatgg
    13021 cacaaaaagc aacggtgccg gtaataactc attgggatca tggtcggagt atggagatta
    13081 ttcataacgc ctggactcat ggtatgaatt cattaatgcg tgatgcttcc gcatttgatt
    13141 tcgaagaaaa tattcgttta accaaagagg ctgttgattt cttccatccg ctgggtattc
    13201 cggtagaggc ggaattaggg catgtcggta atgaaaccgt ttatgaggaa gcgctggcgg
    13261 gctatcacta taccgatcct gaccaggcgg ctgaatttgt tgaacgtacg ggctgtgact
    13321 cattggctgt cgccatcggc aaccagcatg gggtttatac gtcagagcca caattgaact
    13381 ttgaggtcgt caaacgcgta cgcgatgcag tgtctgttcc gctggttttg catggcgcat
    13441 cggggatcag tgatgccgac attaaaactg caatttcgtt gggtatcgcg aaaatcaaca
    13501 tccatacgga gctctgtcag gccgcaatgg tcgcagtgaa agaaaaccag gatcagcctt
    13561 tcctgcattt agaacgtgaa gtacgtaaag ctgtaaaaga acgggcattg gaaaaaatta
    13621 aactgttcgg ttctgatggc aaagcggaat aatgatatgg ataatctcga cgttatttgt
    13681 ataggtgccg ctattgttga tattccattg caaccggtca gtaaaaatat ctttgatgtg
    13741 gattcttatc ctcttgaaag aatcgcaatg accaccgggg gtgatgcaat aaatgaagca
    13801 acaattattt ctcgtctggg ccatcgtaca gcattaatga gccgtattgg taaagatgcc
    13861 gcagggcagt ttattctcga ccattgccgc aaagagaata ttgatattca aagcctgaag
    13921 caggacgtca acatagatac ctctattaat gttggactgg tgacggagga tggcgagcgg
    13981 acgtttgtca caaaccgtaa cggcagtctg tggaaattga atattgacga tgttgatttt
    14041 gcgcggtttt cccaggcgaa attattatca ctggccagta ttttcaacag tccactattg
    14101 gatggtaaag cgctaacaga aatttttacg caagctaaag cccggcagat gaatatctgt
    14161 gccgatatga ttaaaccgcg gttgaatgaa acgctggatg atatttgcga agcattgagt
    14221 tatgtcaatt atgccgaggc aaaattactc accgggaaag agacactgga tgaaattgct
    14281 gacagctttc ttgcgtgcgg cgtaaaaacg gtggtgatta aaacgggtaa agacggctgc
    14341 tttatcaagc gtggtgacat gacgatgaag gtgccggcgg tcgcaggaat aaccgccatc
    14401 gacaccattg gcgcgggcga taactttgct tcaggtttta ttgcggcact gttagaagga
    14461 aaaaatctgc gtgaatgcgc acgctttgcc aatgcaacgg cggctatctc ggttctaagc
    14521 gtcggtgcca ccaccggcgt aaaaaacaga aagctggtcg aacaattgct ggaagaatac
    14581 gaaggataat gaaggcaaat gaaaaagata cctttaggca caacggatat tacgctttcg
    14641 cgaatggggt tggggacatg ggccattggc ggcggtcctg catggaatgg cgatctcgat
    14701 cggcaaatat gtattgatac gattcttgaa gcccatcgtt gcggcattaa cctgattgat
    14761 actgcaccag gatataactt tggcaatagt gaagttatcg tcggtcaggc gttaaaaaaa
    14821 ctgccccgtg aacaggttgt agtagaaacc aaatgcggca ttgtctggga acgaaaagga
    14881 agtttattca acaaagttgg cgatcggcag ttgtataaaa acctttcccc ggaatctatc
    14941 cgcgaagagg tagaagccag cttgcaacgt ctgggttttg attacatcga tatctacatg
    15001 acgcactggc agtcggtgcc gccattttat acgccgatcg ctgaaactgt cgcagtgctt
    15061 aatgagttaa aggctgaagg gaaaattcgc gctataggcg ctgctaacgt cgatgctgac
    15121 catatccgcg agtatctgca atatggtgaa ctggatatta ttcaggcgaa atacagtatc
    15181 ctcgaccggg caatggaaaa cgaactgctg ccgctatgtc gtgataatgg cattgtggtt
    15241 caggtttatt ccccgctaga gcagggattg ttgaccggca ccatcactcg tgattacgtt
    15301 ccgggcggcg ctcgggcaaa taaagtctgg ttccagcgtg aaaacatgct gaaagtgatt
    15361 gatatgcttg aacagtggca gccactttgt gctcgttatc agtgcacaat tcccactctg
    15421 gcactggcgt ggatattaaa acagagtgat ttaatctcca ttcttagtgg ggctactgca
    15481 ccggaacagg tacgcgaaaa tgtcgcggca ctgaatatca acttatcgga tgcagacgca
    15541 acattgatga gggaaatggc agaggtcttg gggcgttaaa tactcaacac gattcacctc
    15601 tgtttctgtg aaaactgtga tgaaaatctg tgattcaaac aggttatttt gaaagtaaac
    15661 atcggttatg cgataatcgc gctaatgtga tgtaaaaaga tacaggggtg taatcgtggc
    15721 ggcaaaagac aggattcagg caattaagca aatggttgcc aacgataaaa aggtgacagt
    15781 ctcaaatttg agtgggattt ttcaggtaac cgaagaaacc attcgccgcg atcttgagaa
    15841 gctggaagat gaaggctttt tgaccagaac ctatggtggt gctgttttaa atacagcgat
    15901 gttgacggag aatatccatt tttataagcg cgcttcatcg ttttatgaag agaagcagct
    15961 tattgcacgc aaggcactac cctttatcga caataaaacc accatggcag ccgattccag
    16021 tagtaccgta atggaattgc tcaaattgtt acaggaccgt agtgacctga cgttgctaac
    16081 caactcggcg gaagcgattc atgttctggc tcagtcagaa attaaagtcg tttcaacggg
    16141 tggggaacta aacaaaaata cactttcact gcaaggaaga attactaaag agatcatcag
    16201 ccgctatcat gtcgatatca tggtaatgag ttgtaaaggt cttgatatta acagcggcgc
    16261 gctggactct aatgaagcgg aagctgaaat caaaaagaca atgatccgtc aggcgacaga
    16321 agttgcgtta ttggttgatc actctaagtt tgatcgcaaa gcttttgtcc agttagccga
    16381 ctttagtcat attaattaca taataactga taaatcaccg ggtgcagagt ggatagcatt
    16441 ttgcaaagac aataatatcc agctggtgtg gtaatttaag cacgcgttgt caatattatt
    16501 tgttcctgcg cctggaaata aaatgatgat aaagatgcgg caagtgaatt tgccgcaatt
    16561 gctacctttt ttagcaaatc cgaggcaccc aatggaacaa tatgatcaaa ttggcgcaag
    16621 actggaccgc ttgcctttgg cccggtttca ttatcgtata tttggtatta taagctttag
    16681 tctgttatta ac
//
LOCUS       DAZFBR010000103        16636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFBR010000103 DAZFBR010000000
VERSION     DAZFBR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.103"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..182
                     /locus_tag="ACHJZ2_003708"
     CDS             <1..182
                     /locus_tag="ACHJZ2_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4569743.1"
                     /translation="DKQYVWCWGKSYGWTECDTFEGYYDCSRNKWWAVTDNGEEPASK
                     VTHWMPLPEPPQEVK"
     gene            270..512
                     /locus_tag="ACHJZ2_003709"
     CDS             270..512
                     /locus_tag="ACHJZ2_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4569744.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            516..650
                     /locus_tag="ACHJZ2_003710"
     CDS             516..650
                     /locus_tag="ACHJZ2_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569745.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            669..923
                     /gene="xisR"
                     /locus_tag="ACHJZ2_003711"
     CDS             669..923
                     /gene="xisR"
                     /locus_tag="ACHJZ2_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4569746.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            957..2243
                     /locus_tag="ACHJZ2_003712"
     CDS             957..2243
                     /locus_tag="ACHJZ2_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4569747.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            2264..2965
                     /gene="mlrA"
                     /locus_tag="ACHJZ2_003713"
     CDS             2264..2965
                     /gene="mlrA"
                     /locus_tag="ACHJZ2_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO4569748.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            3025..3132
                     /gene="yohO"
                     /locus_tag="ACHJZ2_003714"
     CDS             3025..3132
                     /gene="yohO"
                     /locus_tag="ACHJZ2_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO4569749.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(3113..3844)
                     /gene="yehW"
                     /locus_tag="ACHJZ2_003715"
     CDS             complement(3113..3844)
                     /gene="yehW"
                     /locus_tag="ACHJZ2_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO4569750.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(3849..4775)
                     /gene="yehX"
                     /locus_tag="ACHJZ2_003716"
     CDS             complement(3849..4775)
                     /gene="yehX"
                     /locus_tag="ACHJZ2_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO4569751.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(4768..5925)
                     /gene="yehY"
                     /locus_tag="ACHJZ2_003717"
     CDS             complement(4768..5925)
                     /gene="yehY"
                     /locus_tag="ACHJZ2_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO4569752.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLIACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(5932..6849)
                     /gene="osmF"
                     /locus_tag="ACHJZ2_003718"
     CDS             complement(5932..6849)
                     /gene="osmF"
                     /locus_tag="ACHJZ2_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO4569753.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(7101..9398)
                     /gene="bglX"
                     /locus_tag="ACHJZ2_003719"
     CDS             complement(7101..9398)
                     /gene="bglX"
                     /locus_tag="ACHJZ2_003719"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO4569754.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9594..11309
                     /gene="dld"
                     /locus_tag="ACHJZ2_003720"
     CDS             9594..11309
                     /gene="dld"
                     /locus_tag="ACHJZ2_003720"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4569755.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(11347..12279)
                     /gene="pbpG"
                     /locus_tag="ACHJZ2_003721"
     CDS             complement(11347..12279)
                     /gene="pbpG"
                     /locus_tag="ACHJZ2_003721"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO4569756.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(12453..13040)
                     /gene="yohC"
                     /locus_tag="ACHJZ2_003722"
     CDS             complement(12453..13040)
                     /gene="yohC"
                     /locus_tag="ACHJZ2_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO4569757.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            13210..13788
                     /gene="yohD"
                     /locus_tag="ACHJZ2_003723"
     CDS             13210..13788
                     /gene="yohD"
                     /locus_tag="ACHJZ2_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4569758.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(13918..14679)
                     /gene="yohF"
                     /locus_tag="ACHJZ2_003724"
     CDS             complement(13918..14679)
                     /gene="yohF"
                     /locus_tag="ACHJZ2_003724"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4569759.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(14732..16168)
                     /gene="mdtQ"
                     /locus_tag="ACHJZ2_003725"
     CDS             complement(14732..16168)
                     /gene="mdtQ"
                     /locus_tag="ACHJZ2_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO4569760.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            16392..16475
                     /gene="yohP"
                     /locus_tag="ACHJZ2_003726"
     CDS             16392..16475
                     /gene="yohP"
                     /locus_tag="ACHJZ2_003726"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO4569761.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         4106 a         4462 c         4196 g         3872 t
ORIGIN      
        1 acgataaaca gtatgtttgg tgttggggga agtcttacgg ctggactgag tgcgatacct
       61 tcgaagggta ttacgattgt tcgagaaaca aatggtgggc agttactgac aatggggaag
      121 aaccggcatc gaaagtaacc cactggatgc cgctaccgga gccgccgcag gaggtgaaat
      181 gatgaattgg cctgaagcat tcaccgcagt tggagttgca atagcggtgg catttattct
      241 gtattcgctt ttccgctggg gataaaggaa tgttcgctct gattcaacgt ggtcagatat
      301 acacggacag agccggatac cctgtggtga ttactcgcag tactcagcac tcagtgttct
      361 ttcgacgcat ggacgggcgc tccggacggg tacgcattgg tgagttcaac aacctgttcg
      421 aacatattga ccaacaggag taccgcaaaa ttctggcggg cactgagcag gaaatgcgcc
      481 tgaaaaaatt acgcgcaatg caacggaggt gatacatgca tacggctttt gagttctggg
      541 ttcgcaagac attcggcaat cgctacgacc tgacccgtga tgtcgacggc ttctactgcc
      601 gtgaagttgt gaaacgaatg tttgacgtgt ggtgccactg ccgtggatga aagttttatg
      661 aggttggcat gcagacaatc atctatcaga taacccccag caaatggtgt acggagagag
      721 tccttattgc atcaacaggg ctaaagcccg gcaccatcga gcgggccaga agaaagtcat
      781 ggatgcaggg aaaagaatac cgccattacg ctgtagaagg tgatcctggg cattacagtg
      841 aatgcctgta caacatcgaa gaaattatgc gatggatcga aaaccagaaa caaccaggtg
      901 ccaaaaatgc aagttccggt taacctgtta atgctcctgg acgtctggga ggtttaatga
      961 gtaacgcatc atacccgaca ggcgttgaaa accatggcgg atcactccgt atatggtttc
     1021 actataatgg caaacgtgtc agagaaaacc tcggtgttcc tgacacagcc aaaaaccgga
     1081 agatcgctgg tgaacttcgc acttccgttt gttttgcaat cagaatgggg agtttcgact
     1141 acgccgcgca gttccctaat tcccctaacc tgaaacactt tggtctggga aaaagagaga
     1201 taaccgttaa ggcactttcg gaaaaatggt tggaccttaa gaaaattgag atttgtgcga
     1261 atgcacttaa ccgttaccag tcagtaatta aaaacatgtt accaatgtta ggtgaaaaaa
     1321 aactggtttc atccataaca aaagaggatt tacttttcgt aaggagagat ttgttgaccg
     1381 gttaccaaaa gctttctaat ggaaagactt cttccataaa agggcgctca gtggtcacgg
     1441 taaactacta tatgacaacc atagctggaa tgtttcaatt tgcaacagat aatggttata
     1501 cctcaggaaa cccatttaac ggtctggctc ccttaaaaaa gtccaaggta aaaccagatc
     1561 ctctcacccg tgacgaattt attcgtttta ttgaggcttg ccgtcatcaa caaacaaaaa
     1621 acctgtggat tctcgctgta tacacgggta ttcgtcacgg ggagttggta tcgctggcat
     1681 gggaagatat agaccttaaa gcaaggacta taaccatccg tagaaattat acaaaacttg
     1741 gcgaattcac tccaccaaaa accgatgcag gcaccggaag gacaattcat ctggttcaac
     1801 cagctattga tgctcttaaa agccaggcgg aaatgaccat gcttggaaag caacattctg
     1861 tagaggtgaa gcagagggaa tatgggagaa ctgctgtgca taaatgcact tttgttttta
     1921 gtcctcaggt aacaaaacag cagcagttgt ccggacctca ctacaaggtt gactccatca
     1981 gggagtcatg gacaagtatc ttaaaacgcg caggtctgag acacagaaaa tcgtaccaat
     2041 ccaggcatac ttatgcatgc tggtcacttg ccgcaggagc taatcctagt tttatcgcaa
     2101 gccagatggg ccacacaaac gcacaaatgg tattcaatgt ttacggagca tggatgaaag
     2161 acaacaatca cgaacagata gaactcctta acaaaagact atctgaaagt gtcccatgta
     2221 tgccccataa gaaagcaggg taaaataaaa acttgcaaaa tcaattagtt tacccttaat
     2281 ccctgtcacg ttacgcgcgt ggcagaggcg ttacggattg ctgaaaccgc aacggacaga
     2341 cggcggtcat cggctgttca acgatgccga tattgaccgg atccgcgaga tcaaacgctg
     2401 gatcgacaac ggcgtgcagg tcagcaaagt taaaatgctg ctcagtaatg aaaatgttga
     2461 tgtgcagaac ggctggcgcg atcagcaaga aacattactg acctacctgc aaagcggcaa
     2521 tctgcatagc ctgcgaacgt ggatcaaaga gcgcggtcag gattaccccg cccagacgct
     2581 caccacacat ctgtttattc ctctgcgccg acggcttcag taccaacaac cgactctcca
     2641 ggcgctgctg gcgatcctcg acggcgtact gatcaactac atcgccattt gtctggcttc
     2701 ggcacgtaaa aaacagggta aagatgcgct ggtggttggc tggaatattc aggataccac
     2761 ccgtctgtgg ctggagggct ggattgccag tcaacaagga tggcgcattg atgtcctcgc
     2821 ccactcgctc aatcaactac gccctgaact attcgaaggc cgtacattgc tggtgtggtg
     2881 cggtgaaaat cgaacctccg cccaacagca gcaactcacc agttggcaag aacaaggcca
     2941 tgatattttc ccactcggca tttaatgatt cgttaacaaa tgcgctttac tgtacaatcc
     3001 tttcgttaac ataaggagtg cattatgcgc atagctaaaa ttggggtcat cgccctgttc
     3061 ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg caggttatac ctttattttg
     3121 cgtgctggct aagcgcctgc accagccttt caaacaggcg gtctgcgatg atcgccgcca
     3181 gtgccaccag taacgccccc tggatcacat acgcggtatt aaatccgcta agcccgatga
     3241 tgatgggcgt acccagcgtg ctggccccta ccgttgaggc gatcgtcgcc gtaccaatgt
     3301 tgataatcac cgaggttcgc acgcccgcca gaatcaccgg agccgccagc ggtagctcga
     3361 ccttacgcag tcgctgacct cgactcattc ccataccttt cgcaacttct gtcacgctgg
     3421 catcaatcgc tcccagcccg gcaagtgtcg cctgcaggac gggcagcaca ccataaagga
     3481 tcaaggcgat aatcgctggt tgcagaccaa agcctatcac cggaacagca atcgccagca
     3541 ctgcgacagg cggaaaagtc tgcccaacgg cggcaatagt ttccaccagt gggcgaaatt
     3601 ccgcgcccca cgggcgagtg actgcaattc cggcaccagt gccaatgatc accgcaaaca
     3661 aactcgaaat tcccaccagc cagaaatgag ccagtgccaa agctgcaaaa ctttcttgct
     3721 gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa cagcggctgg ctgtaaggca
     3781 gccagaaaat cagtgccaca aacaaagcaa tgagccagaa cagcggatcg cgcaacatct
     3841 tcatacgctt acgcctccac cagcagatcc tgaaaatgca gcgtgccgct aggctggccc
     3901 tgcgtgttca ccaccggcag cacctcgcat ccccgcgcga caaacaggga gagcgcatcg
     3961 cgcagcgtca tctcttctgc cagtgcctca ccttctgccc gttcttcgcg acgcacgtaa
     4021 tccgccacac tacgtaacga aagcaggcgc acacccagtt cactacgtcc aaaaaactgg
     4081 cggacaaaat cattcgccgg acgagtcagc atcgtcagcg gattcccctg ctgcaccact
     4141 tcaccgtgat ccatcaatac cagatgttct gccagccgta gcgcctcatc aatatcatgc
     4201 gtgaccagca caatggtacg ccccagcaaa cggtgaatgc gcgtcatctc ttgttgcaac
     4261 gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat ccatcagtaa gacttgcgga
     4321 tcggcagcca gtgcgcgcgc tactcccaca cgttgctgct gaccaccgga aagctgatgt
     4381 ggataacgct cacgtaaatt tgactccagc cccagtagcg ccattaattc gtcgatacga
     4441 tcgtcaatcc tcgcccgcga ccatttttgt aattgcggca cggtggcaat gttttgtgcc
     4501 acgctccagt ggggaaacag gccaatagat tgaatggcat agcccatacg gcggcgcaac
     4561 tccagtactg gcagcgagcg aatttcttct ccagcaaagc ggagctctcc gctgtcatgt
     4621 tccaccaggc ggttaatcat tttcagggtg gtggatttgc cggagccaga tgtgccaatc
     4681 agcaccgaaa aactcccttc ctgaaaattg agattgagat cgttaacggc tttttgtgcg
     4741 ccgaacagtt tgctgacatg gctaaattca atcattacgt ttcaccttca acagtgcgat
     4801 tagtaaatcg aacagcgcat cgatcagcac cgccagaaca attaccggga tcacccccag
     4861 caacactaaa tcaatggcgc tgcttagcag cccctggaaa accagcgcac caaaaccgcc
     4921 tgcgccgatt aacgccgcaa tcaccgccat gcctacagtt tgcaccatca ccacccgcag
     4981 gctgcgcaga aataccggta acgccagcgg taactgaaca tgcaggaatc gctgcgcccc
     5041 gctcatgccc atcgctctgg cgctctccag cacatcgcgc gggatctggt tcaagccgac
     5101 taccacgccg cgcaccagcg gtagcaaggc atagagcacc agcgcaatca gtgcgggtgt
     5161 cattccggtt cctgctatgc caagcttccc cagccacgga aaggccgtaa ccagtccggc
     5221 aagcggcgca atcagcaggc caaagagcgc cacggaaggc acggtctgaa tgacattgag
     5281 cagagaaaaa atcgccccct gccgcgcggt ggaaaagtag caccagatgc ccaacggcac
     5341 accaatcact aacgcaggca gcaccgcacc aaacagcaac gtcagatgtt gtgccagcgc
     5401 gtcgtcaaac acatcctgac ggttggcgta ttctttcatt agtgagagat cgttaagcgt
     5461 gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca accagcgcca
     5521 cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg ccagcgccga
     5581 agccaaccag aaaccactgc cgaggctggt acgcgccagc gcactgccat tttgcgccag
     5641 ttgggtcgcc gcctttccag ctccccacac cagcaatacg aagacgaatt gcgccagaat
     5701 gagtgcaaaa atgctgcctt ttttagcggg aataaaacag gctatcagcc aggcgcaacc
     5761 aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgac gcccttcacc
     5821 cgagactaaa cgattaggcg cgtagctgat aaacggcagc gctgctgcga ttgccgtcag
     5881 caacagcagc agcgccagaa caggattaat acgtagataa gtcacgggaa attacttcgt
     5941 ccaccctttt tgtttcaagt agtcggcagc cacttttttg gcatccagtc cttccactgc
     6001 aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga ctggctgtag
     6061 ccactgtgcc atttgcggat actctttcag caccgactca cgcaccactg gtgcaggcgc
     6121 gtagataggt tgcacgcctt gcggatcgct taaggtttgc agcccctgcg ccgcgaccgg
     6181 accgtcagtg ccgtaagcca ttgcggcatt aacgccagaa gtttgctggg cagcggcttt
     6241 aatcgttacc gccgtgtcgc cgccagccag tgacagtaac tgatcctgat cgagtttaaa
     6301 gtcgtaggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact cagccgaggc
     6361 tgccagtttg aaggtgccgc cctctttcag ataacgcctc aggtcagcaa gcgaagtgag
     6421 tttgtttttc tctgccacat cctgacgcac ggcgatggtc caggtgttat ttgcaggtgc
     6481 gggcgtcagc cagattaact tgttttgctc tgcatcgagt tttttgactt tctcgtaacc
     6541 ttgctgcgcg tttttccacg ctgcatcgtt ttcatcttta aagaaaaacg cgccattgcc
     6601 ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca ccacaggagt
     6661 cgttccaagt tgcactttat tgaccgttgg tactccgtgg ctttcgagta cttgcaaaat
     6721 gatattgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa cgggggaagc
     6781 cgcgtgcagc ggcaggctca cggctgccaa caaaaccagt gaacctgccc agacctttga
     6841 gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta agagaaaagc
     6901 atagttgata atggcgggat tagcctgagc ggacggattc agttgcagaa tcagataaat
     6961 acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc gccttaaccg
     7021 gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa gggcgcaaac
     7081 gtcgcgccct tgctaaagca ttacagcaac tcaaactcgc ctttcttaac gcgtgcggaa
     7141 tcagtgccga taaagacatt gaacttgcca ggctcggcgt catatttcat ctgttgattc
     7201 cagaacttca gcgcctcgat atcaatcggg aagctgacgg tctgggtttc gcccggcttc
     7261 agggtgattt tctcaaagcc tttcagctgc ttcactggac ggctcatgga agccgtcaca
     7321 tcctgcaagt acatctgcac taccgtggcc ccttcgcgtt taccggtgtt cgtcacctgc
     7381 acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt cacatcagag
     7441 acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc gttagcttca
     7501 tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg accggtgttc
     7561 agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat cggcagctta
     7621 ccggacgggt tgtaatcgcc aaacaacaca tcggcaattg cattaccgcc ttcagtcccg
     7681 gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag cgccagcgga
     7741 cgcccgttca tcagcaccag caccagcggt ttgccggtgg ctttcagggc ggcaatcaaa
     7801 tcacgctggc tttgcagaat agtaatatcg gtacggctgg aggcctcgtg cgccatcccc
     7861 tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg caccgcttca
     7921 tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata ctgattcagg
     7981 aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag cactttaccg
     8041 ttttcaccga cggcattttt aatcccggtc agcacggtca cggattgatc ggcaacacct
     8101 gccgccgacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc aaccaccgca
     8161 atggtggctg attttttcag cggtaacgtt tcgagacggt ttttcagcaa caccaggctt
     8221 tcacgcgcca cttcacgcgc ttctttgcgg tgcaggcggc tttcggcatt ggtatccacc
     8281 gggtcagact ctttcggacc gagatggctg tatgggtcgt taaacaaccc catatcatat
     8341 ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatcgtcac tttaccggat
     8401 ttgatcaacc caggcagata cttcgagtag tactcgtcgc tcatgctcat gttgattccg
     8461 gatttcagcg ccacgcgcac tgcatcttcc ggatctgccg ccgtgccatg tttaatcagc
     8521 tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg gtcgcgcaga
     8581 acatctttca gcagccagga gtcggaggtg gctggcgtgc cgttcagcga gttcagcgcc
     8641 accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat ataatcatta
     8701 aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc taccgcgccg
     8761 tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg gcttttaccc
     8821 tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc ttcgccaaaa
     8881 ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg tgcccaggtc
     8941 atattcaggc catcatctgc cgcttcataa gcagagacac gtccgaccgt ttttactgca
     9001 tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg ctgaccgtgc
     9061 agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat cacctgatcc
     9121 tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tagccccaac ctgaccgtct
     9181 ttgatcatct cgcggatcgc ctctttcggg ttatccgggc cgacgctgat taagcgcagc
     9241 tgaccaattt tctcatcaac tgtcattttc ttaagcagtt cggtgacgaa cgcatcccgc
     9301 gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg ctgcagggcc
     9361 agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct tcctttgttg
     9421 cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat gatggagaga
     9481 gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct gaattgtcat
     9541 acaaggcgct attctagttt gtgatgtttt ttcgccacca caaggagtgg aaaatgtctt
     9601 ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg gtcggtcatt
     9661 cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc cgttctggtc
     9721 atggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg cgggtgctga
     9781 aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca ggcctgaccg
     9841 aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc agcaccctac
     9901 gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat ccgggcacca
     9961 cgctttattc cctggaaaaa gccctcaaac cgctgggacg cgaaccgcac tcggtgattg
    10021 gttcatcgtg cattggcgca tcggttatcg gcggtatctg taataactct ggtggttcgc
    10081 tggtgcagcg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata aatgaagatg
    10141 gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg gagcagatcc
    10201 ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat ggtcgtcacg
    10261 cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg cccgcacgtt
    10321 ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag ctggccgtct
    10381 ttgcagtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt tatatcggca
    10441 ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac ttcgaaaatc
    10501 tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa aaatacggca
    10561 aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc ttctttaatc
    10621 tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg cattttactg
    10681 accgtgcgat gcaaaaattc ggtcacctgt tccccagcca tttaccgcct cgcatgaaaa
    10741 actggcgcga taaatacgag catcatctgc tgttaaaaat ggcgggcgat ggcgtgggcg
    10801 aagccaaatc gtggctggtg gattatttca aacaggccga aggcgatttc tttgtctgta
    10861 cgccggagga aggcagcaaa gcgtttttac accgtttcgc cgctgcgggc gcagcaattc
    10921 gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcgctggat atcgctctgc
    10981 ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc cagctggtgc
    11041 acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac atcgttaaaa
    11101 aaggcgtgga tgtgcatgcg ttgaaagaac agatgctgga gctgctacag cagcgcggcg
    11161 cgcaataccc tgccgaacat aacgtcggtc atttgtataa agcaccggag acattgcaga
    11221 agttttatcg cgagaacgat ccgaccaaca gtatgaatcc ggggatcggt aaaaccagta
    11281 agcggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat cgccatccga
    11341 attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg ctttttgttt
    11401 tttatagctt aacgctgctg ccggaacagg catcacttta ccggtttcaa tccaggtacg
    11461 caggcggctg gcatcggcaa aatgggtgta tttgccaaac gcatccatca ctaccagcgc
    11521 caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg cattggtaaa
    11581 gccggttttg gttagctgga tattccagtt atcgcgatac accagatgat tagtattgcg
    11641 gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg tagtacttaa
    11701 ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt cacgggcggt
    11761 tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt tcattccgag
    11821 cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg gataatggtg
    11881 cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca gcatatcttt
    11941 acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct ccggcgtctg
    12001 gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca cgaccatcgc
    12061 agtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt tcgaatagat
    12121 cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt ccggttgtga
    12181 agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gccaaaggca cagccagcat
    12241 cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc agatagtggt
    12301 tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc gacgccatca
    12361 taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag gaaatgctgc attgctgaca
    12421 tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc gtagccccat
    12481 agtataacgg taagggcgag cagcacttcc agtaccagca cgccaatcgc cagcgtcgaa
    12541 ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat accaaggtaa
    12601 agtaggtaac cggtgtaaaa cagtgccacc gtgccgacca gcgcacacaa ccagaccagc
    12661 ggataaagcg ccaccagacc acttaaaaac agcggagttg caacgtagcc cgcgaagacc
    12721 atgcagtgcg caagtgacgg acgctgcgga taattacgcg ccatccacca gatgacccgc
    12781 cccatcaccg cgaccccagc gagcataacg ccataaaaca ggacagccag cgccagtccg
    12841 gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat ctgtgtagtg
    12901 ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg ggtgtaatgg
    12961 tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg atgggaaaac
    13021 agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct gacagttcaa
    13081 gtataagtca gcttgtgatt attttttgtt cgcccatgta atttgcaccg tctgatgtac
    13141 gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca aagggagaca
    13201 gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc gctggttatc
    13261 ggtagcctgg cggaaggtga aaccgtgact ttgctgggag gccttgcggc acatcagggg
    13321 ctattaaagt tcccgctggt ggtactttct gtagcgcttg gcggcatgat tggcgaccag
    13381 gtgctctatc tgtgcgggcg gcggtttggc ggtaagctgt tacgccgttt ctctaaacat
    13441 caggataaaa ttgagcgggc gcagaaactg atccaacgcc atccgtatct gtttgtcatt
    13501 ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg tgccagccag
    13561 ctgccgccga aaatctttct gccgcttaat attctcggcg catttgcctg ggcgttgatt
    13621 tttaccacta tcggttacgc tggtggtcag gtgattgcgc cgtggttgca caatctcgac
    13681 cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt aggcgtgcgc
    13741 tggtggctga aacgacgcgg gaagaaaaag ccggataatc aggcgtaaaa ccattgccct
    13801 ggataaggcg tgcgcgccgc atccgacatc cggttcacca tgcctgatgc ggcgcttgtg
    13861 cgtcttatca tgcctaccaa aattttaccc attgaaatat ccacacacct cactgcgcta
    13921 ttctgggttg aactgcggat tcgccaacat aaagccgcca tccactatca acgactgccc
    13981 ggtggtgtaa tttgcgccct ccgaacaaag ccacgccacc aggctggcaa tctcatgcgt
    14041 tgcgccaaaa cgccgcagag gaatcgaagg ctccgcgtcg ggcttcacgt cgctatcatc
    14101 catgccgttc attggcgtag cgatcgcccc aggtgcgact gcgttcacca aaatcttatg
    14161 cctgaccaac tccagcgcca ttgctttggt taacccgccg agcgcatgtt tagcggctgt
    14221 gtaggcgctg gcatccggca gcggcgtatg ttcatgtacc gacgtaatgt tgatgatgcg
    14281 accaccctgt ccttgtttca ccatctggcg ggcagcaatt tgcgagcata aaaatgcacc
    14341 atcgacatca acggtaaaaa tcttgcgcca ctcatcgaaa gccatatcaa gaaacggcac
    14401 tttggtcatt gcgcccgcat tattcaccag cacatcaata cgccccagcc gttgaataag
    14461 tttctccagc gcctgcgcac cttctggcag attgccgaga tccagttgca cgatctccgc
    14521 acgtacgccg tggctaacta cctcacgcgc ggtatctttt gccccttctt catctgagtg
    14581 ccaggtaatg ccaatatcaa acccttgctg cgccagtaat aacgcgcact ctttgccgat
    14641 ccccgaatcg gaggcggtaa taatcgctac ctgtgccatc gagttctcca cttaacgctg
    14701 aataaacgtt aagtatagaa gtcacatatc atcagcgttt gtaacccccg cccaacgcac
    14761 cagtgagttg aatggaggca tccagccact gcccttgcaa taacaggcca ttggcccgct
    14821 cacgcagcgc ggggattctg gcttcgctga cgcgggaacc agcaatgatg cccgcgttaa
    14881 agcgggcctg cgcaagacct accacacgca aggcatcgcg ctcaatttgc gcctgatgct
    14941 ggtttttctc cgccagtgtc tgaacctgac tggctgcccg cgccacgtca ttcaccgctt
    15001 caaccaccgc tttgttgtag ctggcgatag acaagttgct ttcggctttt gcgatatcga
    15061 gattggcgtt aagacgacca ctatcgaaaa tgggtagcgt caggcctgcc gtaacgccca
    15121 tttgctgcgc ggaatgacgg aacagatcgc ttaagtgcaa cgcatcctgt tgcaggaagg
    15181 ccatcaggtt gatgtcagga taaaatgccg cttttgccgc atcaatggtg cttagcgatg
    15241 actcaacgta ccagtgcgcc gcctgcaaat ctgcccgccg ggccagtaag gagtacccca
    15301 gttcatcagg aagctggctt gccactttcg gcaacgccac cggtttaagc gtcaatgact
    15361 tagtctggtg atttgtgagt gcaattaacc gtgcctcaat aattttcatt ttccccgcga
    15421 catcgttgag ctgctgccgg gttttgctgg cattaatatc ggtttccaca ccttcaactg
    15481 aagaagtaat cccgttctga tatagctggc gatcggtcgc gataatggtg ttctgctctt
    15541 tttcgatttg ctgcaagacc gtgtttaacg ccgcctgggt ttgccactcc cagtacaggc
    15601 gggctacgct gccagccagc aattggcggg tttgctcgcg ttccgccgcc cgtgctttaa
    15661 ccgtacccag gcgggcagta acctccgccc gattctttcc ccagatatcg agatgccagc
    15721 ccgccgttaa gccaaaagtt ccgttggtgt accacggacc ggtcgtacct gcggccggat
    15781 cgttcagagc aaacggcccc attagccctt ctgccgacat tttttgccgc tccatatccg
    15841 cagaaaagtc gatctgcgga ccatcctgag tagcaactgc cttcgcctgg gcttcagcta
    15901 actgaatgcg ctgttcagcc acctgcatat ccggtgcgtt ctgtagtgca ttgttaatta
    15961 aggaagtgag ttgattatcg tgatactcca gccaccattg gctgtctggc caaccatttt
    16021 tcagcgccgt gggtaatgcg gtgtcaactt gtgcagcggg cgtttgctgg cttaacgcct
    16081 ggcgggtttc atgcataggc gcacacccgg ccagcatcag taacagcgga aaacaggcga
    16141 tggctggata aaaggaatca cgattcatgg gggaataatc aggtaagaaa aggtgcgcgg
    16201 agattaccgt gtgttgcgat atatttttta gtttcgcgtg gcaatacatc agtagcaata
    16261 aaacgacata tccagaaaaa tatacactaa gtgaatgata tcttccgatt tatcttaatc
    16321 gtttatggat aacggcaaag ggcttcgttt tttcctatac ttattcagca ctcacaaata
    16381 aaggaacgcc aatgaaaatt atactctggg ctgtattgat tattttcctg attgggctac
    16441 tggtggtgac tggcgtattt aagatgatat tttaaaaatc agatatgaca tataagttgt
    16501 caggtcagga actagatatt ttgtgtactt gatttgcaaa cagctccggt gttggtggcg
    16561 tcaaaatcgc tgaagcgttc cccgaaggcc ggggcagccc acatggatgt gggctgaggg
    16621 cgcgtcttac aaggac
//
LOCUS       DAZFBR010000104        16390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFBR010000104 DAZFBR010000000
VERSION     DAZFBR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.104"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..260)
                     /locus_tag="ACHJZ2_003727"
     CDS             complement(<1..260)
                     /locus_tag="ACHJZ2_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4569762.1"
                     /translation="MLPIRLKKARLNAGLTQEQLGILVGIDECSASARMNQYEKGKHM
                     PDFSLTKKIADLLNVPVSYFYTPEDDLAEIILLINKLDKKQR"
     gene            complement(428..1702)
                     /locus_tag="ACHJZ2_003728"
     CDS             complement(428..1702)
                     /locus_tag="ACHJZ2_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4569763.1"
                     /translation="MKLNARQVETAKPKDKSYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAEARTRRDEAKKLLAQGIDPKAVQKEAQAENAGAYNFET
                     IAREWHASNKRWSEDHRARVLRYLELYIFPYIGLSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTAKTRHYPALPSHRFPEFLARL
                     AAYRGRMMTKIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHLVPLSRQAIGLLEQLKQISGDKTLLFPGDHDATKVMSENTVNNALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MVQWWADYLYSNREKHISAFEFANKLNKNSLD"
     gene            complement(1867..1951)
                     /locus_tag="ACHJZ2_003729"
     tRNA            complement(1867..1951)
                     /locus_tag="ACHJZ2_003729"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1915..1917),aa:Leu,seq:caa)
     gene            2147..3166
                     /gene="ahr"
                     /locus_tag="ACHJZ2_003730"
     CDS             2147..3166
                     /gene="ahr"
                     /locus_tag="ACHJZ2_003730"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO4569764.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            3296..4798
                     /gene="yjgR"
                     /locus_tag="ACHJZ2_003731"
     CDS             3296..4798
                     /gene="yjgR"
                     /locus_tag="ACHJZ2_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO4569765.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKYLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4959..6041)
                     /gene="lptG"
                     /locus_tag="ACHJZ2_003732"
     CDS             complement(4959..6041)
                     /gene="lptG"
                     /locus_tag="ACHJZ2_003732"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO4569766.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6041..7141)
                     /gene="lptF"
                     /locus_tag="ACHJZ2_003733"
     CDS             complement(6041..7141)
                     /gene="lptF"
                     /locus_tag="ACHJZ2_003733"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO4569767.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(7204..7254)
                     /gene="ytgA"
                     /locus_tag="ACHJZ2_003734"
     CDS             complement(7204..7254)
                     /gene="ytgA"
                     /locus_tag="ACHJZ2_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO4569768.1"
                     /translation="MSIIILATISTTQENK"
     gene            7408..8919
                     /gene="pepA"
                     /locus_tag="ACHJZ2_003735"
     CDS             7408..8919
                     /gene="pepA"
                     /locus_tag="ACHJZ2_003735"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO4569769.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9273..9716
                     /gene="holC"
                     /locus_tag="ACHJZ2_003736"
     CDS             9273..9716
                     /gene="holC"
                     /locus_tag="ACHJZ2_003736"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO4569770.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9716..12571
                     /gene="valS"
                     /locus_tag="ACHJZ2_003737"
     CDS             9716..12571
                     /gene="valS"
                     /locus_tag="ACHJZ2_003737"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO4569771.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12626..13822)
                     /gene="yjgN"
                     /locus_tag="ACHJZ2_003738"
     CDS             complement(12626..13822)
                     /gene="yjgN"
                     /locus_tag="ACHJZ2_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO4569772.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGVWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIVSVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            14015..14518
                     /gene="yjgM"
                     /locus_tag="ACHJZ2_003739"
     CDS             14015..14518
                     /gene="yjgM"
                     /locus_tag="ACHJZ2_003739"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4569773.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(14564..14980)
                     /gene="rraB"
                     /locus_tag="ACHJZ2_003740"
     CDS             complement(14564..14980)
                     /gene="rraB"
                     /locus_tag="ACHJZ2_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO4569774.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            15142..16146
                     /gene="argF"
                     /locus_tag="ACHJZ2_003741"
     CDS             15142..16146
                     /gene="argF"
                     /locus_tag="ACHJZ2_003741"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO4569775.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(16203..>16390)
                     /locus_tag="ACHJZ2_003742"
     CDS             complement(16203..>16390)
                     /locus_tag="ACHJZ2_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569776.1"
                     /translation="VIEEKLNNELKLRFRMMQTILKSEVNVSPFIDQQRLNTLNPPEN
                     LRIAIEKFGWKKKTITA"
BASE COUNT         4261 a         4090 c         4162 g         3877 t
ORIGIN      
        1 aaccgctgtt ttttgtctag tttattaatc aacaaaataa tttctgccaa gtcatcttcg
       61 ggtgtgtaaa aatatgagac agggacattt aataaatcag cgattttttt agtaagtgaa
      121 aaatcgggca tatgtttacc tttctcatat tgattcatgc gagcactggc agagcattca
      181 tcaatgccaa caagtattcc caactgttct tgagttaacc cagcattcaa tcgggctttt
      241 tttaacctaa ttggaagcat cgaaaatcct ctttttggag gtaggtttgc actttttaca
      301 ctcgaaatta ctaaggaata cttagttttt aaaagcaatg gaataattgc ctaagaagtt
      361 gttaagtgaa ggttaaaaag acacaagcat tgctgttgat gaaggctagt tcaaactaag
      421 cggagactta atcaaggcta tttttattaa gtttatttgc aaattcaaaa gcagagatat
      481 gcttctctct gttggaataa agataatctg cccaccattg gaccattagt ctgcgctcat
      541 ctaaatgctc agacgtatga atatacgcag ctcgcacatt attacgttcc gaatggctca
      601 attggcgttc aatagcatca tcactccata atcctgactc tcctaacgca ccacgtgcca
      661 ttgtcctaaa accatgtccg caaacttctg ttttcgtatc atagcccatc gcacgcaatg
      721 cattattaac agtgttctca ctcataacct tagttgcgtc atgatcccca gggaaaagca
      781 gcgtcttatc accactaatc tgctttaact gctccaacaa ccctatcgct tgcctactaa
      841 gtggaacgag atgctcttcc ttcatcttca ttcctctata tgagtaacgt acgcctttaa
      901 tttcctcgcg ttttgcaggt atacgccata gagatttatc gaagtcgaat tcatcccagc
      961 gagcgaaacg tagctcactg gaacgaacaa atgttaataa agaaagttca accgcaattt
     1021 tcgtcatcat acgaccacga tatgcagcaa gacgtgctaa gaactcagga aaacgatgtg
     1081 atggtagagc tggatagtgc cgcgttttag ctgtcgataa cgcaccagcc atatcacttg
     1141 caggatttga atctatataa tcgttctgta cggcatagcg cataatagca gtaacacgtt
     1201 gctgaagacg ctgcgccaca tcatgcttac cactggcatc aacttttttt atcggtgcta
     1261 aaagatggct agttttaagc tgtcggatat cagacaagcc aatataagga aagatataca
     1321 gctctagata gcgaagaaca cgcgcccgat gatcctcact ccaacgtttg ttgctggcgt
     1381 gccattcacg agcaatagtt tcaaaattat aagcaccagc attctctgct tgagcctctt
     1441 tctgtactgc ttttggatct ataccttgcg ccagcaactt tttcgcttca tccctcctcg
     1501 ttctagcttc agcaagcgtc acggtcggcc aaacaccaaa agcgaggcga tcctcttttt
     1561 tgtcagaggg gcgtcggtat ttcatgcgcc agtatttaga ccccttggcc gaaacctcga
     1621 gatacaaacc gccgccatcg gccattttgt agcttttgtc ttttggcttt gctgtctcga
     1681 cttgtctagc attgagcttc atttgggggc acatttctaa tcgaagttaa gatgccccca
     1741 attatgcccc caatgacatc cggatttcaa cggacaacct cggacgatcc aagatcgtag
     1801 aaatcgctga aagtattgat ttttaatgga taatcggact tactcggatg gacttggaag
     1861 taataatggt gccgaaggcc ggactcgaac cggcacgtat ttctacggtt gattttgaat
     1921 caactgcgtc taccgatttc gccacttcgg cactgaagag gtatgcggaa aacgttgtgg
     1981 attatacctg tcgcacgcca ccatgcaagc gccagcttgc tcgcacctcg ctaagtgctg
     2041 aaattttcag cattgcatac agcgatgtgt aacctttgtc acactccagg caccccgccc
     2101 tgccatgctc tacacttccc aaacaacacc agagaaggac caaaaaatgt cgatgataaa
     2161 aagctatgcc gcaaaagaag cgggcggcga actggaagtt tatgagtacg atcccggtga
     2221 gctgaggcca caagatgttg aagtgcaggt ggattattgc gggatctgcc attccgatct
     2281 atcgatgatc gacaatgaat ggggattttc acaatatccg ctggttgccg ggcatgaggt
     2341 gattgggcgc gtggtggcgc tcgggagcgc cgcgcaggat aaaggtttgc aggtcggcca
     2401 gcgtgtcggg attggctgga cggcgcgtag ctgtggtcac tgcgacgcct gtattagcgg
     2461 taatcagatc aactgcgagc aaggtgctgt gccgacgatt atgaatcgcg gtggctttgc
     2521 cgagaagttg cgtgcggact ggcaatgggt gattccactg ccagaaaata ttgatatcga
     2581 gtccgccggg ccgctgttgt gcggcggtat cacggtcttt aaaccactgt tgatgcacca
     2641 tatcactgct accagccgcg ttggggtaat tggtattggc gggctggggc atatcgctat
     2701 aaaacttctg cacgcaatgg gatgcgaggt gacggccttt agttctaatc cggcgaaaga
     2761 gcaggaagta ctggcgatgg gtgccgataa agtggtgaat agccgcgatc cgcaggcact
     2821 gaaagccctg tcggggcagt ttgatctcat tatcaatact gtgaacgtca gcctcgactg
     2881 gcagccttat tttgaggcgc tgacctatgg cggtaatttc catacggtcg gtgcggttct
     2941 cacgccgttg ccggttccgg cctttacgct gattgcgggc gatcgcagtg tctctggctc
     3001 tgctaccggt acaccttatg agttgcgtaa gctgatgcgc tttgccgccc gcagcaaggt
     3061 tgcgccgact accgaattgt tcccgatgtc gaaaattaac gatgccatca agcatgtgcg
     3121 cgacggtaag gcgcgttacc gcgtggttct gaaagctgac ttctgatctc tttgccgggt
     3181 gcctgtcgcc cggcacttaa atctctttgg aaggcgtctc gcaatctgct gaatttagcg
     3241 gtgaagagtg cgcagccgtt gcctatactc caggcaggaa actggaggat atctcatgag
     3301 cgaacccctg ttaattgccc gcacgccgga cacagaacta tttttactgc cgggaatggc
     3361 taaccgtcac gggctgatta ctggcgcaac ggggacgggt aaaactgtca cgctgcaaaa
     3421 actggcggag tcattgtcgg aaatcggcgt gccggtgttt atggctgatg tgaaaggcga
     3481 tctgaccggt atcgcgcagg caggaacggc gtcggaaaaa ctgctcgcaa ggcttaaaaa
     3541 tatcgacgtc aatgactggc aaccgcatgc taatccggtg gtggtgtggg atatctttgg
     3601 cgagaaaggc cattcggtac gggcgacggt ttcagacctg gggccactgt tgctggcgcg
     3661 gctgttgaat ctcaacgatg tgcaatctgg cgtgctgaat atcatcttcc gcattgctga
     3721 cgatcagggg ctgttactgc tcgactttaa atatctgcgg gcgattaccc agtacatcgg
     3781 cgataacgcc aaatctttcc agaatcagta cggaaatatc agtagcgcat cggttggtgc
     3841 catccagcgc gggctgttgt cgctggaaca gcaaggcgca gcacacttct ttggtgagcc
     3901 gatgctggat atcaaagact ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat
     3961 cctcagcgcc gagaaacttt atcagatgcc gaaactgtac gccgccagcc tgctgtggat
     4021 gctctcagag ttgtatgaac aattgccgga agcgggcgat ctggagaaac caaaactggt
     4081 gtttttcttc gacgaagcac atctgctgtt taacgatgca ccgcaggtac tgctggataa
     4141 gattgagcag gtgataaggc ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca
     4201 aaacccgtct gatattccgg ataatgtgct cggccagctc ggtaatcgcg ttcagcacgc
     4261 tttgcgggct tttacgccca aagatcagaa agcagtaaaa gctgcggcgc aaaccatgcg
     4321 ggccaatccg gcgtttgata ccgaaaaggc gattcaggaa ctgggcaccg gcgaggcgtt
     4381 gatctctttt ctggatgcga aaggaagccc ttctgtggtg gagcgtgcga tggtgatcgc
     4441 gccttgttcg cggatggggc cggtgacgga agatgagcgt aatggcttga ttaatcactc
     4501 tccggtgtat ggcaaatatg aggatgaggt ggaccgggaa tccgcctatg agatgttgca
     4561 aaaaggcttt caggccagta ccgagcagca aaataatccc cccgcgaaag ggaaagaggt
     4621 agcggtggat gacggcattc ttggtggatt gaaggatatt ttgttcggca ctaccggacc
     4681 acgcggcggg aagaaagatg gtgtggtgca aacaatggcc aaaagcgccg ctcgccaagt
     4741 gacgaatcag attgtacgcg ggatgttggg gagtttgctg ggggggagaa gaaggtaagt
     4801 caggcgagat ttgtctttgc ctgatgcgac gctggcgcgt cttatcatgc ccaccccact
     4861 gcaatatatt gaattttaat tatttttcag gaatgaacga agcacacttt gctaacaata
     4921 ataaagggag ctttcgctcc ctttattcgt tcattcggtt acgattttct cattaacagc
     4981 cacaggctga ttaagaagaa gctggcgctt ggcaacagtg cgccgatgat cggcgggatg
     5041 ccataaacca acgtcagcgg gccgaagatc tggtccagta cgtagaagac aaaaccgaaa
     5101 ctgataccgg tgaccacacg cacgcccatc ggtacgctac gcagtgggcc aaagatgaac
     5161 gacagcgcca tcagcatcat caccgccacg gatagcggct ggaagatttt gctccacatg
     5221 ttgagctgat aacgtccggc atcctgaccg ctcgacttca gatacttcac atagttgtgc
     5281 aaaccgctga tagagagtgc atccgggtcc agcgccacca cgcccagttt gtccggcgtg
     5341 aggttggttt tccaggtgcc gctcaccgtc tgcgaaccgg taatctgttt cggattggtc
     5401 agatcagatt catcaacctg cgacaaacgc cagactttat gttccgggtc aaacttcgca
     5461 gtagcggcat agcgtacgga ttgcagacga cgattctcgt taaaggcata aatgctgatg
     5521 ccacctaact cttcgtcacc tttaacccgc tcaatgtaga cgaagttgtt gccatctttc
     5581 gcccataagc cttgctgggt agagagcaac gagccgccgt acatcgcctg cgcacggtag
     5641 ttacgcgcca tctgctcgcc ctgcggcgcg acccattcgc caatcgccat cgtcagcaag
     5701 accagcggaa tggcggtttt catcaccgac agcgccacct gcatacgggt aaaaccagaa
     5761 gcctgcatca ccaccagttc gctgcgctgc gccagcatcc caagaccaag caacgcccca
     5821 agcagagccg ccatcgggaa gaatatttgc acatctttcg gcacgctcag caaggtatac
     5881 attcctgcgc ctaacgcgtc gtaactcccc tgcccggctt ttttcaactg atcgacaaac
     5941 ttgataatgc ccgacagcga caccagcatg aacagcgtca tcatgatggt ggtgaaaata
     6001 gttttaccga tatagcggtc aagtacgcca aaaggttgca tcacaccgct cctttacgcg
     6061 aaaaactggc gcgcaggcgg cggaccggca cggtgtccca aaggttgaga acaatcgcca
     6121 aagccagata aatcaggtta acggtccaca tccacagcgt cgggtccagc ttacctttac
     6181 cgccgttcga tttcagggag gtctggatca ggaagaaaag tagatacagc agcatggctg
     6241 gcagcatcga cagtacgcgt ccctgacgtg ggttaaccac gctcagcggt acgaccataa
     6301 gtgccatcat aaacacggtg aataccaacg tgatacgcca gttcagttct gcgcgagcac
     6361 gatcggtgtc agtgttccac aatgtgcgca tgtccatctg gtcggtatcg ttcgggtcga
     6421 gcgccaccgc ctggtgacca atgatcgcct gataatcctg gaaatccgta atgcggaaat
     6481 cacgtaacag tgcagtgcct tcgaagcgcg ttccctggtt gagagtgacg acctgggagc
     6541 cgtcgcgcag ctgggttaaa tgtccggaat cggccaccac cacagaagga cgtgcattac
     6601 cttttggtcg aatttgcgcg aggaacacat ctttgaaatc gctgccgtca acgctttcga
     6661 tgaacagcac cgaactgcca ttagtcgctt gctggaattg cccttgcgcc agcgccgcca
     6721 tgccagggtt cgctttcgct tctgctaaca cttcatcctg atgacgcgat gaccacggtc
     6781 ccgcccacat cacgttaacc gccgcgacga ttgccgtgaa taccgcaagg atcattgccg
     6841 ctttcaccag aaccgctttg ctcaggccgc aggcatgcat taccgtaatt tcactttcgg
     6901 tatacagttt gcccagcgtc atcagcagcc cgaggaacag gcttaatggc aggataagct
     6961 gcgccatttc cggcacgccc aacccgagaa gggagagcac cagattcgcc ggaatatcgc
     7021 cgtcaaccgc tgcgccgagg atcctcacta acttttgaca gaagaagatc aaaagcaaga
     7081 tgaagagtat cgccagctgg cttttgagcg tctcccgcac cagatatctt atgattatca
     7141 ctttaaatac gcccgtaaaa actcgtcttt tgcaggattt tagcttgttt catggcttaa
     7201 acgtcattta ttctcttgag tcgtcgaaat cgtcgctaag ataattatac tcaacggatt
     7261 cacctctcag attttgttct gacgtgccaa tgccgtaata acgttaagat taacacgaag
     7321 tcatcgcaac agcggacatg agttacgaaa gcttgcaatt ctatctgtag ccaccgccgt
     7381 tgtctttaag attcaggagc gtagtgcatg gagtttagtg taaaaagcgg tagcccggag
     7441 aaacagcgga gtgcctgcat cgtcgtgggc gtcttcgaac cacgtcgcct ttctccgatt
     7501 gcagaacagc tcgataaaat cagcgatggg tacatcagcg ccctgctacg tcggggcgaa
     7561 ctggaaggaa aaccggggca gacattgttg ctgcaccatg ttccgaatgt actttccgag
     7621 cgaattctcc ttattggttg cggcaaagaa cgtgagctgg atgagcgtca gtacaagcag
     7681 gttattcaga aaaccattaa tacgctgaat gatactggct caatggaagc ggtctgcttt
     7741 ctgactgaac tgcacgttaa aggccgtaac aactactgga aagtgcgtca ggctgtcgag
     7801 acggcaaaag agacgctcta cagtttcgat cagctgaaaa cgaacaagag cgaaccgcgt
     7861 cgtccgctgc gtaagatggt gttcaacgtg ccgacccgcc gtgaactgac cagcggtgag
     7921 cgcgcgatcc agcacggtct ggcgattgcc gccgggatta aagccgcgaa agatctcggt
     7981 aatatgccgc cgaatatctg taacgccgct tacctcgctt cacaagcgcg ccagctggct
     8041 gacagctaca gcaagaatgt tatcacccgc gttatcggcg aacagcagat gaaagagctg
     8101 gggatgcatt cctatctggc ggtcggtcag ggttcgcaaa acgaatcgct gatgtcggtg
     8161 attgagtaca aaggcaacgc gtcggaagat gcacgcccaa tcgtgctggt gggtaaaggt
     8221 ttaaccttcg actccggcgg tatctcgatc aagccttcag aaggcatgga tgagatgaag
     8281 tacgatatgt gcggtgcggc agcggtttac ggcgtgatgc ggatggtcgc ggagctacaa
     8341 ctgccgatta acgttatcgg cgtgttggca ggctgcgaaa acatgcctgg cggacgagcc
     8401 tatcgtccgg gcgatgtgtt aaccaccatg tccggtcaaa ccgttgaagt gctgaatacc
     8461 gacgctgaag gccgcctggt actgtgcgac gtgttaactt acgttgagcg ttttgagccg
     8521 gaagcggtga ttgacgtggc gacgctgacc ggtgcctgcg tgatcgcgct gggtcatcac
     8581 attaccggtc tgatggcgaa ccataatccg ctggcccatg aactgattgc cgcgtctgaa
     8641 caatccggtg accgcgcatg gcgcttaccg ctgggtgacg agtatcagga acaactggag
     8701 tccaattttg ccgatatggc gaacattggc ggtcgtcctg gtggggcgat taccgcaggt
     8761 tgcttcctgt cacgctttac ccgtaagtac aactgggcgc acctggatat tgcaggaacc
     8821 gcctggcgtt ctggtaaagc aaaaggcgca accggtcgtc cggtagcgtt gctggcacag
     8881 ttcctgttga accgcgctgg gtttaacggc gaagagtaat tgcgtcaggc aaggctgtta
     8941 ttgccggatg cggcgtgaac gccttatccg acctacacag cactgaactc gtaggcctga
     9001 taagacgcgc aagcgtcgca tcaggcaatg ctgttattgt cggatgcggc atgaacgcct
     9061 tatccgacct acacagcacc gaactcgtag gcctgataag acgcggcagc gtcgcatcag
     9121 gcaatgctgt tattgtcgga tgcggcgtga gcgccttatc cgacctacac acagcactga
     9181 actcgtaggc ctgataagac acaacagcgt cgcatcaggc gctgcggtgt atacctgatg
     9241 cgtatttaaa tccaccacaa gaagccccat atatgaaaaa cgcgacgttc taccttctgg
     9301 acaatgacac caccgtcgat ggcttaagcg ccgttgagca actggtgtgt gaaattgccg
     9361 cagaacgttg gcgcagcggt aagcgcgtgc tcatcgcctg tgaagatgaa aagcaggctt
     9421 accggctgga tgaagccctg tgggcgcgtc cggcagaaag ctttgttccg cataatttag
     9481 cgggagaagg accgcgcggc ggtgcgccgg tggagatcgc ctggccgcaa aaacgcagca
     9541 gcagcccgcg cgatatattg attagtctgc gaacaagctt tgcagatttt gccaccgctt
     9601 tcacagaagt ggtagacttc gttccttatg aagattctct gaaacaactg gcgcgcgaac
     9661 gctataaagc ctaccgcgtg gctggtttca acctgaatac ggcaacctgg aaataatgga
     9721 aaagacatat aacccacaag atatcgaaca gccgctttac gagcactggg aaaagcaggg
     9781 ctactttaag cctaatggcg atgaaagcca ggaaagtttc tgcatcatga tcccgccgcc
     9841 gaacgtcacc ggcagtttgc atatgggtca cgccttccag caaaccatca tggataccat
     9901 gatccgctat cagcgcatgc agggcaaaaa caccctgtgg caggtcggta ctgaccacgc
     9961 cgggatcgct acccagatgg tcgttgagcg caagattgcc gcagaagaag gtaaaacccg
    10021 tcacgactac ggccgcgaag ctttcatcga caaaatctgg gaatggaaag cggaatccgg
    10081 cggcacgatt acccgtcaga tgcgccgtct cggcaactcc gtcgactggg aacgtgaacg
    10141 cttcacgatg gacgaaggcc tgtccaatgc ggtgaaagaa gttttcgttc gtctatataa
    10201 agaagacctg atttaccgtg gcaaacgcct ggtaaactgg gacccgaaac tgcgcaccgc
    10261 tatctctgac ctggaagtgg aaaaccgcga atcgaaaggt tcgatgtggc acatccgcta
    10321 tccgctggct gacggtgcga aaaccgcaga cggtaaagat tatctggtgg tcgcgactac
    10381 ccgtccggaa accctgctgg gcgatactgg cgtagccgtt aacccggaag atccgcgtta
    10441 caaagatctg attggcaaat atgtcattct gccgctggtt aaccgtcgta ttccgatcgt
    10501 tggcgacgaa cacgccgaca tggaaaaagg caccggctgc gtgaaaatca ctccggcgca
    10561 cgactttaac gactatgaag tgggtaaacg tcacgcgctg ccgatgatca acatcctgac
    10621 ctttgacggc gatatccgtg aaagcgccca ggtgttcgat accaaaggta acgaatctga
    10681 cgtttattcc agcgaaatcc cggcagagtt ccagaaactc gagcgttttg ctgcacgtaa
    10741 agcggtcgtt gctgcggttg acgcactcgg cctgctggaa gaaattaaac cgcacgacct
    10801 gacagttcct tacggcgacc gtggcggcgt agttatcgaa ccaatgctga ccgaccagtg
    10861 gtacgtacgt gccgatgtac tggcgaagcc ggcagttgaa gcggttgaga acggcgacat
    10921 tcagttcgta ccgaagcagt acgaaaacat gtacttctcc tggatgcgcg atattcagga
    10981 ctggtgtatc tctcgtcagc tgtggtgggg tcaccgtatc ccggcatggt acgacgaagc
    11041 gggtaacgtt tatgttggcc gcaacgaaga agaagtgcgc aaggaaaata acctcggtgc
    11101 cgacgttgcc ctgcgtcagg acgaagacgt tctcgacacc tggttctctt ctgcgctgtg
    11161 gaccttctct accctaggct ggccggaaaa caccgacgca ctgcgccagt tccacccaac
    11221 cagcgtgatg gtatctggtt tcgacatcat cttcttctgg attgcccgca tgatcatgat
    11281 gaccatgcac ttcatcaaag atgaaaatgg caaaccgcag gtgccgttcc acaccgttta
    11341 catgaccggt ctgattcgtg acgacgaagg ccagaagatg tccaaatcca agggtaacgt
    11401 tatcgacccg ctggatatgg ttgacggtat ttcgctgcca gaactgctgg aaaaacgtac
    11461 cggcaatatg atgcagccgc agctggcgga caaaatccgt aagcgcaccg agaagcagtt
    11521 cccgaacggt attgagccgc acggcaccga tgccctgcgc ttcaccctgg cggcactggc
    11581 gtctaccggt cgtgacatca actgggatat gaagcgtctg gaaggctacc gtaacttctg
    11641 taacaagctg tggaacgcca gccgctttgt gctgatgaac acggaaggtc aggattgcgg
    11701 cttcaacggc ggcgaaatga cgctgtcgct ggcggaccgc tggattctgg cggagttcaa
    11761 ccagaccatc aaagcgtacc gcgaagcgct ggacagcttc cgcttcgata tcgccgcagg
    11821 cattctgtat gagtttacct ggaaccagtt ctgtgactgg tatctcgagc tgaccaagcc
    11881 ggtaatgaac ggtggcaccg aagcagaact gcgcggtact cgccatacgc tggtgactgt
    11941 actggaaggt ctgctgcgcc tcgcgcatcc gatcattccg ttcatcaccg aaaccatctg
    12001 gcagcgtgtg aaagtacttt gcggtatcac tgccgacacc atcatgctgc agccgttccc
    12061 gcagtacgat gcatctcagg ttgatgaagc cgcactggcc gacaccgaat ggctgaaaca
    12121 ggcgatcgtt gcggtacgta acatccgtgc agaaatgaac atcgcgccgg gcaaaccgct
    12181 ggagctgctg ctgcgtggtt gcagcgcgga tgcagaacgt cgcgtaaatg aaaaccgtgg
    12241 cttcctgcaa accctggcgc gtctggaaag catcaccgtg ctgcctgccg atgacaaagg
    12301 tccggtttcc gttacgaaga tcatcgacgg cgcagagctg ctgatcccga tggctggcct
    12361 catcaacaaa gaagatgagc tggcgcgtct ggcgaaagaa gtggcgaaga tcgaaggcga
    12421 aatcagccgt atcgagaaca agctggcgaa tgaaggcttt gtcgcccgcg caccggaagc
    12481 ggtcatcgcg aaagagcgtg agaagctgga aggctatgcg gaagcgaaag cgaagctgat
    12541 tgaacagcag gctgttatcg ccgcgctgta atcaaaacac agtgatgaaa acgaaggccg
    12601 gagcatgctc cggccttttt atctcttaca gaaatggtaa agaaggcata attccccgtg
    12661 aaatactggc taagaagcct ttatctggtt gttcttcttt attgattaaa ggaaggtcat
    12721 ccaaatcgcc cagcaaatac gtattttttg cctgccagtc aatcatccat attttcagca
    12781 gtggataagc cagaccgccc gtaatcccgg atatcaccac caacgcacac atgcgataaa
    12841 gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg cccatcattc agtgacaggt
    12901 tgctcataaa atgatttcgc aacgacaccg ttaaataact ggtgctaaca gcaatcccaa
    12961 aataatagat taactgcgcg attatcattt tacgttgcat ttccataaat tgctgtaaat
    13021 tctcaatatc atcatttgca tacacagaac tatcatacgc atttaatatt tgatcgaaga
    13081 taatataacc agcaacagcg agaaaaggta ataaagctaa aaatgcgagg atggcatatt
    13141 ttatacaata cgcagtatct aatttcacct tgaaacgatg tataccgaaa ctggtattgc
    13201 tccagagaaa actcattacc aggctatata aagtaccatt aaaaatacca atggaaacaa
    13261 taccaactat tgccatcaga accacagata caataacact acttgaacta ttggcatgta
    13321 gcatctttgt cgagataaag aaaacagtcc ccatcccaat ggccattaaa atcggcaaga
    13381 aaaaggttac ccaccagacc cctttcatag agcaattaaa actaaatctt acgccgttga
    13441 gactggtcat caaggcctga taacgcagtc cttttgctgc cataaatata agcaaaacca
    13501 acagtgacaa agtcaaaaca cagccaataa gcggcatatc tgctgacact gtcattaaga
    13561 ttgcgaaata gaaaaaaaca aaaacaagac aactaaaaaa aacattccca ccagtaattc
    13621 cataagaaaa tcgttgtccg ttaacttcca tattagcata gagataacgc ttacatttca
    13681 ttaatgccca tggtaaatag atacctagcg ttataatcgt taacaacaca ttcaccacac
    13741 aaataagaaa atatctctca cctgtaccat gaaaaacaaa cgaatgggac ggaacatcca
    13801 tttcattaat aacttgagcc ataaaaattc cctgaattaa aaaatccatt gatataattt
    13861 atagatacag acagtaaaat gttctgccac gttacaatac tcttatcagt aataggctgg
    13921 caaaaacttt attgctttat aagacgcttg cgcacgtaag aagagagtgg tattaaacaa
    13981 atgtataatt actttatttt tattgggtta aactatgaat aacattgcgc cgcaatcacc
    14041 tgtaatgcgt cgcctgacgc tgcaggataa tcctgctatc gcccgcgtca ttcgtcaggt
    14101 atccgccgaa tacggtctta ccgctgataa aggctacacc gtcgccgatc cgaatcttga
    14161 cgagctgtat caagtgtata gtcagccggg tcatgcatat tgggtggttg agtacgaggg
    14221 tgaagtggtc ggcggcggtg ggattgcgcc attagcaggt agtgaatcgg acatttgcga
    14281 actgcaaaag atgtattttc ttccggctat tcgcggcaaa gggctggcaa aaaaactggc
    14341 cttaaaggcg atggaggagg caagagaaat gggcttcaaa cgctgctatc tggaaacgac
    14401 cgcttttttg aaagaagcga ttggtttgta tgaacattta ggttttcaac atatcgacta
    14461 tgcacttggc tgcacgggcc atgttgattg tgaagtgcgg atgctacgcg aactctaaaa
    14521 aaatgcccgc tagtgtgtgc tatcgggcat tttaatcaat taattagtgg cgaaccccgt
    14581 catcgtcttc atcgacaaaa tcttcatcgt cgccgtcttc gccgttggga tcttcaaagt
    14641 aagtgcccca accgtcgtac tcaacgtcaa atttctccgc cagcgtcatc agttgttcaa
    14701 cctgggcatc gatcagatcg gcattcaacg cgcactcgct gaggatgtcg cagcaaatca
    14761 cgatatcacc atcttcaact tccagctctt ctggatcggt cacttcgtaa ccgagtttaa
    14821 acgcttcaac tgctgctttt tccagggttt ccagatcgtc tgcggaaaga tggtgttcga
    14881 tggtgtacag cgcgtccgga tcgctgccat cttccagtaa ttcttcaata atcaaacgtg
    14941 tttcttcacg ctgttcttcc agttgttccg ggtttgccat ggctcattcc tcttaatgtc
    15001 ctgccgatac tcatattgtc acacaccact ggcattgcct ccacctttgt gacaaagatt
    15061 tatgctttag acttgcaaat gaataatcat ccatataaat tgaattttaa ttcattgaga
    15121 cgttagccac aggagggatc tatgtccggg ttttatcata agcatttcct gaaattactc
    15181 gatttcacgc cagctgaact caacagcctg ctgcagttag ccgcgaagct gaaagccgat
    15241 aagaaaagcg gcaaagaaga agccaaactc actggtaaaa acatcgcgct catcttcgaa
    15301 aaagactcga cccgtacccg atgctctttc gaagttgccg catatgacca gggtgctcgc
    15361 gttacttatc tcggcccaag cggcagtcag attggtcata aagagtcgat taaagacact
    15421 gcccgcgtgc tgggtcgcat gtatgacggt attcagtatc gcggctatgg tcaggagatt
    15481 gtcgaaacac tggcggaata cgctggcgtg ccggtatgga acggcctgac caatgagttt
    15541 caccccacgc agttgctggc ggatctcctc accatgcagg agcatttacc cggtaaacca
    15601 ttcaacgaaa tgacgctggt ctatgcaggt gacgcacgta acaacatggg caattcgatg
    15661 ctcgaagctg cggcgcttac cggtctggat ttgcgtctgg tcgcgccaca ggcatgctgg
    15721 ccggaagctg cgctggttac ggaatgccgc gccctggcac aacaaaatgg cgggaatatt
    15781 acgctgacgg aagatgtagc taaaggcgtt gaaggtgctg actttatcta taccgatgta
    15841 tgggtgtcga tgggtgaagc aaaagaaaaa tgggctgaac gcattgcatt actgcgtgat
    15901 tatcaggtga acagcaagat gatgcagttg accggtaacc cggaggtcaa attcctccac
    15961 tgcctgcccg cgttccatga tgaccaaacg acgcttggca agaaaatggc ggaagagttt
    16021 ggcctacatg gcggaatgga agtgactgat gaggtcttcg aatctgccgc cagcattgtt
    16081 tttgatcagg cggaaaaccg catgcacacc atcaaagcgg tgatggtcgc gacactcagt
    16141 aaataattga tatcgctata aagatgccgg gaaatacaga aacatcccgg cgtcaaatta
    16201 ttttatgcag tgatagtttt tttcttccag ccaaattttt ctattgctat acgtaaattt
    16261 tccggaggat ttagtgtatt taaacgctgt tggtcaataa atggcgatac attaacctcc
    16321 gatttcaaga tagtttgcat cattctaaac ctaagtttta attcattatt aagtttttct
    16381 tcaataacat
//
LOCUS       DAZFBR010000105        15911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFBR010000105 DAZFBR010000000
VERSION     DAZFBR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.105"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(49..240)
                     /gene="yaiA"
                     /locus_tag="ACHJZ2_003743"
     CDS             complement(49..240)
                     /gene="yaiA"
                     /locus_tag="ACHJZ2_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO4569777.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYENPDKE"
     gene            complement(290..814)
                     /gene="aroL"
                     /locus_tag="ACHJZ2_003744"
     CDS             complement(290..814)
                     /gene="aroL"
                     /locus_tag="ACHJZ2_003744"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO4569778.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(997..1455)
                     /locus_tag="ACHJZ2_003745"
     CDS             complement(997..1455)
                     /locus_tag="ACHJZ2_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO4569779.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1575..2384
                     /gene="proC"
                     /locus_tag="ACHJZ2_003746"
     CDS             1575..2384
                     /gene="proC"
                     /locus_tag="ACHJZ2_003746"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO4569780.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(2401..3516)
                     /gene="adrA"
                     /locus_tag="ACHJZ2_003747"
                     /gene_synonym="dgcC"
     CDS             complement(2401..3516)
                     /gene="adrA"
                     /locus_tag="ACHJZ2_003747"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO4569781.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3618..3938)
                     /gene="psiF"
                     /locus_tag="ACHJZ2_003748"
     CDS             complement(3618..3938)
                     /gene="psiF"
                     /locus_tag="ACHJZ2_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO4569782.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(4057..5472)
                     /gene="phoA"
                     /locus_tag="ACHJZ2_003749"
     CDS             complement(4057..5472)
                     /gene="phoA"
                     /locus_tag="ACHJZ2_003749"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO4569783.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(5573..5833)
                     /gene="iraP"
                     /locus_tag="ACHJZ2_003750"
     CDS             complement(5573..5833)
                     /gene="iraP"
                     /locus_tag="ACHJZ2_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO4569784.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(6015..6218)
                     /locus_tag="ACHJZ2_003751"
     CDS             complement(6015..6218)
                     /locus_tag="ACHJZ2_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569785.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            6296..7390
                     /gene="ddlA"
                     /locus_tag="ACHJZ2_003752"
     CDS             6296..7390
                     /gene="ddlA"
                     /locus_tag="ACHJZ2_003752"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4569786.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGFKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(7414..7626)
                     /locus_tag="ACHJZ2_003753"
     CDS             complement(7414..7626)
                     /locus_tag="ACHJZ2_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO4569787.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            7886..8194
                     /locus_tag="ACHJZ2_003754"
     CDS             7886..8194
                     /locus_tag="ACHJZ2_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO4569788.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(8253..9347)
                     /gene="yaiW"
                     /locus_tag="ACHJZ2_003755"
     CDS             complement(8253..9347)
                     /gene="yaiW"
                     /locus_tag="ACHJZ2_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO4569789.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(9360..10580)
                     /gene="sbmA"
                     /locus_tag="ACHJZ2_003756"
     CDS             complement(9360..10580)
                     /gene="sbmA"
                     /locus_tag="ACHJZ2_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO4569790.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            10932..12089
                     /gene="ampH"
                     /locus_tag="ACHJZ2_003757"
     CDS             10932..12089
                     /gene="ampH"
                     /locus_tag="ACHJZ2_003757"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO4569791.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(12090..12713)
                     /gene="iprA"
                     /locus_tag="ACHJZ2_003758"
     CDS             complement(12090..12713)
                     /gene="iprA"
                     /locus_tag="ACHJZ2_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO4569792.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(12801..15743)
                     /gene="ehaB"
                     /locus_tag="ACHJZ2_003759"
     CDS             complement(12801..15743)
                     /gene="ehaB"
                     /locus_tag="ACHJZ2_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO4569793.1"
                     /translation="MHSWKKKLIVSQLALACTLAIASQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMITSECMTTDCADDRATGYVYDRLTLSVDNSTIDDNYEHYTYNGTYN
                     NAADTHVVDVYDMGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNS
                     SEVFNNTITVKDSTVTSGSWTDEGTTGWFGHTGNASNYSNTLTADDVAIAAIANPYAD
                     NAMQTTVTLDNSTLMGDVVFSSNFDENFFPQGANSYRDADGDVDTNGWDGTDRMDVTL
                     NNGSKWVGAAMSVHMVDEDGDGSYDGYAVGTEATATLLDIAANSLWPSSTVGVDNINT
                     QYDENGHIVGNEVYQSGLFNVTLNGGSEWDTTKSSLIDTLSINSGSQVNVADSRLISD
                     TVSLTGGSNLNIGEDGHVATNTLTIDNSTVKMSDDVSAGWGLEDAALYANTITVTNDG
                     LLDINVDQFDANPFQADTLNLTSTTDTNGNIHAGVFDIHSSDYVMDTDLVNDRTNDTT
                     KSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRIDN
                     ATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTD
                     YANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINY
                     DQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFA
                     NNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPY
                     GSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYV
                     YDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSA
                     NVGVKYTW"
BASE COUNT         3970 a         4050 c         3798 g         4093 t
ORIGIN      
        1 gatcccttgt agtggcactc agaatccctt ccgagcagtc tgatgcggtc actctttgtc
       61 agggttctca tagcgttttt tcgcatccat cgcttcctga gcggtcggat gttcactgat
      121 caacgagtct ggtttaggat gatcggctct gagttggtac caggttaccg tctgtcctgg
      181 ctttcctttt tcaattgtca ctatatatgc ttcacgagga taaggcggtt tcgttggcat
      241 cgttcttcct tatttcacgg gatgaacgtt aagtataggc gctcgaaaat caacaattga
      301 tcgtctgcgc cagggcgctg cgaatttcag aaatcacctg gctgggttcg tttgttgcgt
      361 cgatgataat atgcgcaact tcgcgatata gcgcatcgcg ttcttccagc acttcctgaa
      421 cttcttcgct cagcggtttt cccgttaagg ttggccgtaa atcttcttcc ggtgcagctt
      481 gcagtcggtt aaccaggact gatactggcg cacacaaata aaccacgatc ccgttatttt
      541 gcatgaagtg acgattaaat tccgtcagaa taatgccgcc gcctgtagcg ataacggtgg
      601 atggcgcagt taccgcttcc agcgccgccg tttctctggc gcgaaatccc gcccactctt
      661 ccctttcgac gatctccgcg accgtcatat tgagctgtga ttgcaaccac tgatcggtat
      721 cgacaaaccg acggttaagc gaatcggcaa gggccattcc gaccgttgtt ttaccacagc
      781 cccgaggccc gatcagaaaa agaggttgtg tcatcgtggg ttttccccaa taggtcgcaa
      841 tgcggcgaaa gccggtgtca tgagaatagc gatcatacca tcaaactagt acaatttcga
      901 ttgtaaagaa aaaattccac ttaaagtgaa aatctcaata catcccttac tataccaata
      961 aatattcaag aatgaagtgt aaataataaa ttacatttag ccacgactac gttgcacttc
     1021 cagccaccac ttctccagct ccgcggcaaa ggcctggcgg tcacgttgtg aaaggctatc
     1081 tggtccgccg gtctggatcc cactggcacg taaggtatcc ataaaatcgc gcatcgtcag
     1141 gcgctcacga atggtcgctg gcgtgtaacg ttcaccgcgc ggattaagcg ccgcagcgcc
     1201 tttctcgatg gcttcagcag ccaaaggtat atctgcggtg atcaccaaat ctcccgcttc
     1261 acactgccgg acaatttcgt tatcggcaac gtcgaaacct gccgcgacgc gcagcgtacg
     1321 aataaatcgc gatggcggca cgcgtaaact ctggtttgct accagtacca gcggcatctg
     1381 catacgttcc gccgcgcgat acaaaatctc tttaattaca ttgggacacg cgtcggcatc
     1441 cacccaaatt gtcataaagt catcctttgt tgggtaatcc tctattgtgt cgcgcttttg
     1501 ccttccggca tagttctgtt tatgcttctg ccagcgatta tcaaaacaat gaatttcacg
     1561 gcaggagtga ggcaatggaa aagaaaatcg gttttattgg ctgcggcaat atgggaaaag
     1621 ccattctcgg cggtctgatt gccagcggtc aggtgcttcc agggcaaatc tgggtatata
     1681 ccccctcccc ggataaagtc gccgccctgc atgacaagtt cggcatcaac gccgcagaat
     1741 cggcgcaaga agtggcgcaa atcgccgaca tcatttttgc cgccgttaaa cctggcatca
     1801 tgattaaagt gcttagcgaa atcacctcca gcctcaataa agactctctg gtcgtttcta
     1861 ttgctgcagg tgtcacgctc gaccagcttg cccgcgcgct gggccatgac cggaaaatta
     1921 tccgcgccat gccgaacact cccgcgctgg ttaatgccgg gatgacctcc gtaacgccaa
     1981 acgcgctggt aaccccagaa gataccgctg atgtgctgaa tattttccgc tgctttggcg
     2041 aagcggaagt aattgctgag ccgatgatcc acccggtggt cggtgtgagc ggttcttcgc
     2101 cagcctacgt ctttatgttt atcgaagcga tggccgacgc cgccgtgctg ggcgggatgc
     2161 cacgcgccca ggcgtataaa tttgccgctc aggcggtaat gggttccgca aaaatggtgc
     2221 tggaaacggg agaacatccg ggggcactga aagatatggt ctgctcaccg ggaggcacca
     2281 ccattgaagc ggtacgcgta ctggaagaga aaggcttccg tgccgcagtg atcgaagcga
     2341 tgacgaagtg tatggaaaaa tcagaaaaac tcagcaaatc ctgatgactt tcgccggacg
     2401 tcaggccgcc acttcggtgc ggttacgtcc ggctttcttt gctttgtaga gcgccaaatc
     2461 tgccgatttc aaccactcgc gatagtgact catttgtggg ttcagcggcg caacccccac
     2521 actaatccgt aaatttacct gtggcgtatt cggcaaacgt aatgtattta gcccttcatg
     2581 cacccgtaac atggcggtaa tggcgctctc agctggcgta ccggacatga ttaccgcaaa
     2641 ctcatcgccg ccaaaccgac caatcacatc gctaccgcgc agggtaattt gtaactgtcg
     2701 ggtaagcgcc acaatcgctt catcgcccac atcatggccc caggtatcgt tgatgctctt
     2761 gaaatggtcg atatcgataa tcagtaacgt tgcatcgcga ttatgccgcc gacagttatc
     2821 aaattcattg cgtaacatag tttcccaatg acgtcggtta tacacgcccg tcatgccgtc
     2881 gcgggtactc atgacctgca acctgcgttt atgttccgcc agtttggttg ccgtctggta
     2941 gctgacccag ccaaacagca gaggataaat gacaataatg ggaagggaga gccaccattc
     3001 cagcggcgca ctattgaacg acacggtaat gcccgtcagc tcgagggtga caaggcagga
     3061 aaccaccatc aacaccagac ccgcgacaaa cagacggggg ccgcctgccc ccatcaaatt
     3121 cagacacata atcatcaaca tcgcggtgga aggcagcacg tttacgccca ttacgcctac
     3181 ccacattccc gctaatactg catcggtttt taagttgtaa atttcccggc taagcggatc
     3241 gacggccctg ctcgctatct gccaggctaa atgcggccag acgaacgccc agccgaccaa
     3301 caccagccac caccagcccg gcggcgggtg tgaaaccagc gttgaagcaa tcggtaagaa
     3361 catgccagcc aggccaaccg cacggggtag tctgacgcga cgggcgaagc gcagcccgga
     3421 ccgctgatgt tcgttttgag gagttaaagg aggttcctcc ccgtgcgccg ccgctttttt
     3481 gaaaaagttt tcatcattca ttatttttgg gaacattcag aaacaatttt cccaaattat
     3541 agagacgggg ctaaaggcag aagtatgaaa tttcgggtga gccattttag tagctcaccc
     3601 gttagcacta tcaggtatca ggcggctttc ttgaggcagg cgctcataaa cttattacga
     3661 tcatcacctt tcagtgattg ttgcgttgct tgattattac attcgcgcat cttttgctgc
     3721 tgtggcgtca aacttttttc accaggcgca gacttgctgt tcttcaggca atcactcatg
     3781 taggtcttac gagcatcccc tttcaacgcc tgtgccgtcg cctgctgatt acaggaggtc
     3841 atacgctgtt gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc
     3901 agaccgaaaa gcaaggtaac cagtaatgta attttcatag caccatccct cttcatgttt
     3961 taaccatgag cgtatgcgcc cgtgatctga cattaagtct ggttgctaac agcaaaaaaa
     4021 ccacccggca gcgaaaattc actgccggga gcggttttat ttcagcccca gagcggcttt
     4081 catggtgtag aagagatcgg tctggtcggt cagtccaacg acattggcgg catgcgggcc
     4141 atacgccgca atacgcaact gactgccggt atgttcttgt gaatcctctt cggagttccc
     4201 gtaactcatc accatcactg cgccatcttt ggtatttagc gcctgagtga ggcccggagc
     4261 tttggtatcc ggcgcaacaa tctggctggc gtgggcgtga tcagcggtga ctatgaccag
     4321 cgtgttacca tcctttttgg cgaattccag cgcccgttgt acggcttcat cgagatcgac
     4381 cgtctcgcca atttgcccac aaggattcgc agcatgatcc tgtttatcga ttgacgcacc
     4441 ttcaacttgc aggaaaaagc ctttctcatt tttactcaac aattcaatgg ctttgtcggt
     4501 catctgtgcc agggtcggta cactgtcatt acgttgcgga ttaggcgtac aggtgactgc
     4561 gggcttatcg atattaccgt ggtacgttgc tttcggtcct agccagcgca ctggcatatt
     4621 gccgtcagca aacaggccaa gcaggggttt ttgctgattc gcttccgtca ccgaattcag
     4681 tgaggcagca tcgctcacca actgataacc acgcgcctgt gcctgttcac gcagcgtttt
     4741 tccctgccat tcaccagcgg ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt
     4801 aacgtcggca cgagcgttaa gcagctgttc ggtaatcgat ccttttccgc ctttttccag
     4861 agcgttaccc ggacattttt cactggtcgc gctcggaccg tagcatttgc gcgaggtcac
     4921 atgcgccacc agcgcggcgg gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt
     4981 cgccagacct gcggcttttg ccatttccag aatcgttggg tgatcttttt cgtgaatatc
     5041 gacgcccagc gcgccgttat aggttttgac accggttgac caggcggttg ctgatgcagc
     5101 cgagtcggtg acgtagtccg gttttccggt ttttttattc agcgcatagt gagtgtattg
     5161 cccggtaagc ggtaaggcat cgataccttt aaaaaagccg cccgcacctt cggcataatt
     5221 acgtgcggca gtaatttccg aatcccccat cccatcgcca atcagcaaaa taatattttt
     5281 tgcaggttta tcgctaagag aatcacgcag agcagcggtc tgatcagccg ttaagcggcg
     5341 agcaccgccg ggtgcagtaa tatcgccctg agcagcccgg ttttccagaa caggcatttc
     5401 tggtgtccgg gcttttgtca caggggtaaa cagtaacggt aagagtgcca gtgcaatagt
     5461 gctttgtttc actttatttt ctccatgtac aaatacatta aaaaataaaa acaaagcgac
     5521 tataagtctc ggccgtgaca actttatgac agctgttgaa aagattaact ttttactgac
     5581 gaggatgctt caataacttc tttacgtaat cgcgcagcag ctccgtatcg tcgtcaggaa
     5641 tgctggcatc gggctttacc tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt
     5701 ggtcattttg cgccatattg cgaagcattg cagtgacgat aatctccaaa gcttctacct
     5761 gcgcacacag ttctttcgac tcttcttctt tttgggcaag cttaaataac aactcagcaa
     5821 tgagattttt catgtctgta tttccttatc caaagtatgg agaagttaac attatgtttc
     5881 acaacttcat agtgtctgga ggtattattt ttatggcaaa ggaaatactt tgcgcaaata
     5941 ttttttgttt gattaccagc gaaacgtttc tcttatttaa ataattaccc acacaaaaca
     6001 taggctttta attatcaacc tttattataa ataaaagtta tgaaatatat tctcttcgtt
     6061 aacttataat taagagaaaa aaactcaccg catggccatt taagccagga ataaattagc
     6121 ttcccgttca tcatttttgt gatcttgcgc acatctttga gataacgcac tttcttcacc
     6181 agtacgttgc acgctttgtc agcagaagat tgcaacaatc tttcagaagg ccgcgaatcc
     6241 cccgttttgt cgtggtaatg tatgcacttt gtgttagacg ggataggttt ttaagatgga
     6301 aaaactgcgg gtaggaatcg tttttggtgg taaatcagcg gaacatgaag tgtctctgca
     6361 atcggcaaaa aacattgtcg atgccattga taaaagtcgc ttcgacgttg tgctgctggg
     6421 cattgataaa caagggcaat ggcacgtcag cgatgccagc aattatctgc taaatgcaga
     6481 cgatcctgcc catattgcgt tgcgcccttc ggcgaccagc ctcgcccagg tgccaggtaa
     6541 acatgagcat cagcttatcg acgcgcaaaa cggtcagccg ttgccgacgg tggatgtcat
     6601 tttcccgatt gtccacggta cgctgggcga agatggttcc ttgcagggaa tgctgcgggt
     6661 cgccaattta ccgtttgtag gttctgatgt tctggcttca gcagcctgta tggataaaga
     6721 tgtcaccaaa cgtctgctac gcgatgccgg gctgaacatt gcgccattta ttaccctgac
     6781 gcgcgctaat cgtcacaaca tcagttttgc cgaagtggag tctaaactgg ggttaccact
     6841 gtttgtaaaa ccggctaatc agggctcttc tgttggtgtc agcaaagtaa ccagtgaaga
     6901 acaatacgca attgccgtcg atctggcgtt cgagttcgat cataaagtga tcgttgagca
     6961 agggttcaaa ggtcgtgaga tcgaatgcgc agttctgggc aacgacaatc cgcaagccag
     7021 cacctgtggc gagatcgtac tcaccagcga tttctatgcc tacgacacca agtacattga
     7081 cgaagatggc gcgaaagtgg tagttccggc agccattgcg ccagaaatca acgataagat
     7141 ccgggcgatt gccgttcagg cttatcagac gttgggatgc gcaggcatgg cgcgtgtaga
     7201 cgtgttttta accccagaga acgaagtggt gatcaacgag atcaacacac tgcctggctt
     7261 caccaacatc agtatgtatc cgaagctgtg gcaagccagc ggtctgggtt acaccgatct
     7321 gatcacacgt ttgattgagc tggcgctgga gcgtcacgct gcggataacg cattgaaaac
     7381 cacaatgtaa tattttaacg cccgataata ttcttattgg gcgttttcag tttcttcatc
     7441 gcgacgacga ataatcaacc ccgccagcca gaaactaatc acccacgtca ccagaccgac
     7501 ggcataggtc tgccaacctt ttgcttcaaa tcccagtaac cccaccacgc cattaagaat
     7561 gaatataagg ccaatggcga acgcatagta gtgccagtca cggcggattt ttacaggcag
     7621 attcatggct gctccgttaa cgaaaaacaa catccttgca tattctgcaa caaccgtctg
     7681 tggtgtagac aacattttcg caattgttaa taaatttaac ttaaagttgc tattttgtga
     7741 ctcgcaacga agattcacgc ttcgggaata ttcccaacat caatttacca tgattctgat
     7801 attgacaatc taaatgacct gtcagacttg ccataagccg ctggtatcac accagaagag
     7861 aagcagatga cttccggagg tttttatggc tgatttcacc ctgtcaaaat cgctgtttag
     7921 cggaaaatat cgcaatgcct cttcaacgcc tggcaacatt gcctatgcgt tgtttgtgct
     7981 gttttgcttt tgggctgggg cgcaattgct gaacctgtta gtgcatgcgc ccggcgtcta
     8041 tgagcgttta atgcaggtcc aggaaacagg tcgcccacgg gtggaaattg gtttaggtgt
     8101 cggcaccatt ttcgggctga tcccgttttt agtaggctgc ctcatttttg cagtggtggc
     8161 gctatggctg cactggcgac atcgccgcca gtaagattta cgttgccaga tacggcctaa
     8221 aacgccttat ctggcatgac attcctgttt gttcactgtt tcatacaacg cgcccgccgt
     8281 tcgtctacgc gcttcgcaaa ccaggccgta gtcaggttgc gggtgatttt cgggctttcc
     8341 agttgaatgc caggcaacat ttcgcggggg agtgatttac ccgttttcgc ttcggcaagt
     8401 tgataaactt tcttgtacag cgccgtctcc tcaaaagaga agctgtcacc tttctctagc
     8461 tgacgacgaa tttcgctgtc gctcatcccc agttttgccg ccagtttgcg cgttgccagt
     8521 tcggttttcc cgggttcttt actgtcgtaa cgaattaaat caccgtccag tgccagcttc
     8581 acgccgctgg ccttactgac cgcgttctga aacgcggcat tacggctggc gtaccagcca
     8641 gcgttaaaat cagcaaaacg gtatatcggt gcgctataac tggcgggata attcagtaaa
     8701 tggtaagtac caaaccacaa cccgccgcgg cgactaaaga cttcctgacg gactgtaccg
     8761 tccattttcc acggataccc tttggtatgc tgttcggcaa aagcaatgct gacctgcatc
     8821 ggcccaccgg tgcgcaccgg attaagcgaa ccaaacaacg tctgccccat tggcaccatg
     8881 ctgatcagat cgtcaaaaat cccgctcaat tgcttttctg tacgcaccga atctaaccgt
     8941 tcgctataac ttttgccgtt tggcgatttg atttttagcg cagtatgaac cagaaaagca
     9001 ggaatgtgca tccgttcagc acgacggtca atctcttgcc aggcgatctt gcttaagccc
     9061 ggaacggccg gatctgcctg ataatttgac tcctgctgcg ccaccgccag caccgagcag
     9121 acgttttcca gcgtcggtgc cagcccctga ctttcaaagg tggtggcgag atctttcgcc
     9181 caggcatcgc ggtctttcac gctcgcaggc atcttctgcc gcaccacgct agccacatct
     9241 atcgcttttt cgcccttttt caacggctgt ggcgcctgac tgctacaccc cgctaaaacc
     9301 agaaccgcca aaaatgaaag cgaagacaga tgattgactc gcgacattac ttctcctttt
     9361 tagctcaagg tatgggttac ttcctgaatt ttgtcaccat ccagctcatg ttcaaagctg
     9421 cgcagacgtt tgtagataga catcaactca accagtgtgg tccatgagtt aatcaggtac
     9481 tggaaagcac cgcgaacctg accaaaaacg ttggtaatct gcgtcatcag gccgagcgta
     9541 atcgtaccgg caacaattga cggaaacagc aagaacaaac cgaaaacgtt atcgacctgc
     9601 aaatagagaa tgcgagcgat gttgaaatac atatagtgaa aatagaggcg gaaatagttt
     9661 ttccgtacgg cgctaaacag ctcgcgtacc gttggcggcg tcgcgcgcgt ggcatcgtct
     9721 tcaccataaa ccagctcttt acggtaggca gcctctacac gctggttttt aaactccagc
     9781 cccggcagtt tgatccctac cactgccagc aatccggtcc ccatcagcga ccagacgata
     9841 gcggcaatca ccagaccata cggaatgtgc ccgataatcg gcagctccgg cacatgcgcg
     9901 gagagcgtta ccaacaccgg caggaaggcg atcaacgtca tgatggcgtt gataaaactg
     9961 acacccatat tctccagcgt tgaagcaaaa cgcatggtgt cttcctgcac acgctgtgcg
    10021 gcgccttcga tatgacgcag ttgttgccag ttcgccatgt aatattcgtt catcgccgta
    10081 cgccagcgga acacgtagtg actgacaaag aagttattca gcacactgat cactaccgcg
    10141 atcagcgcaa tccccagaaa gacgcccact tcgcggtaaa attgttcgat agtgacttta
    10201 tgcggcgaac ttagcgcggt ttgaatcaga tcatagaacg gtgcatacca ggcgttgacg
    10261 gcaaccccaa cttccaccaa aaaccaggtg acgaagatga tcagtgcagt accgagtatt
    10321 gaccagtatt gccaacggtg cgggctgtag ataaaccaga acaatgcaaa aagtcctacg
    10381 caaacaatgt agtaagcgta aaaaatcagg aaatccaacg accagaaacg cgcggcgcta
    10441 atcgggatct gcccggaagc gccggtgata cgcgccaccc agtccccccc accggcttgc
    10501 cagaagataa cggcaatcaa tgcccaaaca aaggccgaga gaaaaaacgt tcccggcttt
    10561 gggaaaaaag acttaaacat atgcactcct gctaacttct tatcgtttta acaactgctg
    10621 tgtattaacc gcatgaccgc tacaaagcgt cagccaaatg aaaccgccca ttgtgactca
    10681 tagcaaggcg cttagttcgc cagcctggcc ataagaattg tttcgacgga tttcacaaac
    10741 caaagattgt ttacataagc ggattaacac gcgcaggatc acaaatgcag gcattggtga
    10801 gcaggaaaaa gatcgtgttt acgcgccgtt acgtttttaa cactattatt gcactaattc
    10861 gccctttgca atccatcaat gagtagtata aatacgctca gttaccttca ttcaatttat
    10921 ggacaccacc gttgaaacgt agtctgcttt tttctgccgt gctgtgtgcg gcgtcattga
    10981 cctccgtcca tgcggcgcag ccgatcactg aaccggagtt tgcctctgat attgtcgatc
    11041 gttatgccga tcatattttt tacggcagcg gtgccacggg gatggcgctg gttgttatcg
    11101 acggtaacca gcgcgtcttt cgcagttatg gcgaaacgcg acctggtaat aacgttcgcc
    11161 cgcagctgga ttcggtcgtg cgtattgctt ccctcaccaa gctaatgacc agtgagatgc
    11221 tggtgaaatt gctcgaccag gggaccgtga agttaaacga tccgctaagt aaatatgctc
    11281 cgccaggcgc acgcgtgcca acctacaatg ggacgcccat tacgctggtc aatctggcaa
    11341 cccataccag cgccctgccc cgtgaacagc ccggtggcgc ggcacatcgt ccggtgtttg
    11401 tctggccaac gcgcgagcaa cgctggaaat acctttctac ggcgaagctg aaagccgcgc
    11461 caggcagtca ggcggcgtac tctaaccttg cgttcgacct gctggcagat gcactggcga
    11521 atgcgtccgg caagccttat acccagttgt ttgaagagca gattacccgt ccattgggga
    11581 tgaaagacac cacctacact ccctcaccgg atcagtgccg ccgtttaatg gttgctgagc
    11641 gcggtgccag tccgtgcaat aacacgctgg cggcaattgg cagcggcggc gtctattcca
    11701 cgcctggcga tatgatgcgc tggatgcagc agtatttgtc atctgatttt tatcagcgca
    11761 gtaaccaggc agatcgcatg cagacgttga tttatcagcg cgcgcagttt acgaaagtga
    11821 ttggcatgga tgtccccggc aaagcggatg cgcttggttt aggctgggta tatatggccc
    11881 cgaaagaggg tcgtccgggg attattcaga agacaggtgg tggtggcggt ttcattacct
    11941 atatggcgat gatcccgcag aagaatattg gcgcgtttgt ggtggtcact cgttcgccgt
    12001 tgacgcgctt taaaaatatg agcgatggca tcaatgatct ggtgaccgag ctaagcggga
    12061 ataaaccgtt ggttatcccc gcatcctgat taatactctg aaggcaaacg gttgatagcg
    12121 accagtttgc ctttattcat ttcgatatag ccgcctttac gcaaagcagc gagaacttct
    12181 gcgacgacag aacgcgatat gcgtgtacgt tgatggatat aattcatcac accaatacgt
    12241 gatcgcaatt cttcattcca gtcaatcatt gataataatg ttgcgcggat ttgttcgtag
    12301 gaattatgcc caatgagctg cacgtcgcgt aattccagaa ttctattttg ccaggctaac
    12361 cagtaaaaag cgtctcgcca gagttgattt tgttcaataa gcgtaatggt ttgtttggct
    12421 ggtagatgat atcccgtaca atttccttct gatattaatt tgtatggtat atcgtttttc
    12481 attaaaccat cagccagccc cataatataa ggagcctggg taataccgat aagtacgttt
    12541 tcttctctac gcagagagat aactccctct aaaataacga aagtatcttc gttattgaca
    12601 tcactggaaa atataacttg cttttcatta ttaaactcga agcgcgtacc gtatctggac
    12661 aaacatttat cgagcttacc aaattcctga agaggtttaa ctacagataa catttgcgcg
    12721 tcctttgcag taatgcccgt caaatccttg acgggcatta tttagattaa attaccagta
    12781 tttcttcgga gtgaagaata ttaccaggta tatttaacac ccacgttcgc ggaccagtct
    12841 tgatctacgt caccaccacc gaggtagtta gcatcggtat aggcgctgaa gttcttggtg
    12901 aagctgaact gagtacccag accaacacgt accgcagacc cttcagtacc gttatcgatg
    12961 gaatcaccgt tcacatcgtt atcgttgtta gagtcgtcgt agacgtaagc cagtttgaag
    13021 tacggagtca gagcctgatc ttcgctgtag gtgaaggtat aacctgcatc tacacccagt
    13081 tcataacgca tgctgtcgta agactgaccg tcaactttca tgtcgttgct cagctggtag
    13141 tcatcaccag actggaacag accagaaatg ctgccgtaag gagtcacgta accagcatca
    13201 cccagtttga agtcgtaacc ggctttcaaa ccgaagcccc aagcgtcgga gttggtgcta
    13261 ccgtcaacgt aagtaccgtt gctcatggtt gcagacaggt cgttgttgaa gtgagagtag
    13321 ctcaagctac catcaacaaa gacgttgttc gcgaagtgag cagaagagta gatgtaggca
    13381 gtctggctgt cttgatccac ctgaccagaa cggtcattca tgtcaccttt agcgaagcct
    13441 gcagccgcac cgacgatcca cttagcgttg ttaccgtcaa ttttggtatc aacaccgacc
    13501 atgatgccgt taacatcctg atcatagttg atggtgccgt tgtcgccgtt gaagttacca
    13561 ccgaagtagc ttacccatgc gccgccgtta tcagccaggc catgacgaga gttggtcaaa
    13621 cgagtaccaa cggtgtcttg ttccaggttc cagatattgg tgttcgcaga tgggatgctc
    13681 agcgccatgt tagcgtagtc ggtcaactcc atctgttgca gaacaacggt gttaccgcgc
    13741 tgttcagcct gataggtgta tgcacccagg tcagctttgt tagcagcaga gaaggtcgcg
    13801 ttgctgtttt tgtcgttgac gtagatcagc tcgttgccgt tgtagtcagc gatagaacca
    13861 gcaccagtag cgttgtcgat acgaactttg tagttaccgg tcgcggctgc aacatggtca
    13921 ccgttgttat caacttcgtt ctgaccagct tcgatagaag cagtgttgtc gttatcgccg
    13981 ttaccgttaa tagtcaggtg accatcagag ttcattgcga ttaagccata accgtagttt
    14041 gacttggtag tatcgttggt acgatcgttg accagatcgg tatccattac gtaatcactg
    14101 ctatggatat cgaatacacc agcgtgaatg ttgccgttag tatcagtggt actggtcaga
    14161 ttcagggtat cggcctggaa cgggttagca tcgaactgat caacgttaat atccaacaga
    14221 ccgtcgttag ttacggtgat ggtatttgcg tacagtgcag catcttctaa accccagccc
    14281 gcagaaacat catcagacat tttaacggta ctattgtcga tggtcagggt attagtcgct
    14341 acatgaccgt cttcaccgat gttcaggtta gaaccgccag tcagagagac agtgtcagag
    14401 atcagacgag agtctgcaac attaacttgg gaaccgctgt taatacttaa agtatcaatc
    14461 agagaagatt ttgttgtatc ccactctgaa ccaccgttca aagtcacatt aaacaaaccg
    14521 ctctggtaaa cttcgtttcc tacgatatgg ccattttcgt catattgagt attgatgtta
    14581 tcaacaccga cagttgatga aggccacagg ctgttagctg caatatcgag cagagttgca
    14641 gttgcttcag taccaacagc atatccgtcg taagaaccat caccatcttc atcaaccata
    14701 tgaacagaca ttgcagcgcc aacccacttg ctgccgttgt tcagagtcac atccatacgg
    14761 tctgtgccat cccaaccgtt ggtatctaca tcaccatcag catcgcgata gctgttagca
    14821 ccttgcggga agaagttttc atcgaaatta ctggagaaaa caacatcacc catcagtgtt
    14881 gagttgtcta aagttactgt agtctgcatc gcattatcag catacggatt tgcgattgcg
    14941 gcaattgcaa catcgtctgc agtcagcgtg ttgctatagt tgctggcatt accagtatgg
    15001 ccaaaccaac cagtagtacc ttcatcagtc catgaaccag aggtcacagt agaatcttta
    15061 acagtaatag tgttattaaa cacttcgctg ctgtttacgc cagtgctgac tgtgttgtcg
    15121 tcaatatctt cccactcata accctgagtc agcgtaatac ctgctacatg agagttatta
    15181 gtgatggaca gatcaacttc ctgatccagt gtaatagcag tacccatatc gtaaacatct
    15241 acaacatgag tgtcagcggc attattatag gtaccgttgt aagtataatg ctcgtagttg
    15301 tcatcgatcg ttgaattatc aacgctcagt gtcagacgat cataaacata accagtagca
    15361 cggtcatcag cacaatcagt agtcatgcac tcagaggtaa tcataccgtg aatagtgctg
    15421 tttttgattg tcagactatt ggcgttggta ttggtagaaa taccgtcgtc cagatagtag
    15481 gtagaaatca cgccgttaac ggtagcgtta ttaatgaccg ggtagatatc gccgttgtaa
    15541 tagctatcag cggcatagtt gttccagcct acataaccat catagtaaac gtcatcagca
    15601 taggtggcgt cgttgtagtg atggaaagta ttgtaagttt gaccagaaat atcgttggtc
    15661 gctgcattag cctgagaggc gatagccaga gtgcaagcca atgctaattg tgatactata
    15721 agtttctttt tccaggagtg catttgtcat ccctcctcag ggacgtaata agttgatcca
    15781 tcaattatca atgcatagaa aacatcttat gtttcgcgtt gacgattatg cggcgttata
    15841 gaggaatggt tcaattattc tttcccctaa gtccgattgc ggacatatat aaaaatatct
    15901 atacgcacaa a
//
LOCUS       DAZFBR010000106        15911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFBR010000106 DAZFBR010000000
VERSION     DAZFBR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.106"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..1346)
                     /gene="uidB"
                     /locus_tag="ACHJZ2_003760"
     CDS             complement(<1..1346)
                     /gene="uidB"
                     /locus_tag="ACHJZ2_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO4569794.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQ"
     gene            complement(1343..3156)
                     /gene="uidA"
                     /locus_tag="ACHJZ2_003761"
                     /pseudo
     CDS             complement(1343..3156)
                     /gene="uidA"
                     /locus_tag="ACHJZ2_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            complement(3547..4137)
                     /gene="uidR"
                     /locus_tag="ACHJZ2_003762"
     CDS             complement(3547..4137)
                     /gene="uidR"
                     /locus_tag="ACHJZ2_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO4569795.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4366..5133)
                     /gene="hdhA"
                     /locus_tag="ACHJZ2_003763"
     CDS             complement(4366..5133)
                     /gene="hdhA"
                     /locus_tag="ACHJZ2_003763"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO4569796.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5245..6273)
                     /gene="malI"
                     /locus_tag="ACHJZ2_003764"
     CDS             complement(5245..6273)
                     /gene="malI"
                     /locus_tag="ACHJZ2_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO4569797.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6448..8040
                     /gene="malX"
                     /locus_tag="ACHJZ2_003765"
     CDS             6448..8040
                     /gene="malX"
                     /locus_tag="ACHJZ2_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO4569798.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8050..9222
                     /gene="malY"
                     /locus_tag="ACHJZ2_003766"
     CDS             8050..9222
                     /gene="malY"
                     /locus_tag="ACHJZ2_003766"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO4569799.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9326..10327
                     /gene="add"
                     /locus_tag="ACHJZ2_003767"
     CDS             9326..10327
                     /gene="add"
                     /locus_tag="ACHJZ2_003767"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO4569800.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(10363..11403)
                     /locus_tag="ACHJZ2_003768"
     CDS             complement(10363..11403)
                     /locus_tag="ACHJZ2_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4569801.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11646..11771
                     /gene="blr"
                     /locus_tag="ACHJZ2_003769"
     CDS             11646..11771
                     /gene="blr"
                     /locus_tag="ACHJZ2_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO4569802.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            12044..12259
                     /gene="ydgT"
                     /locus_tag="ACHJZ2_003770"
     CDS             12044..12259
                     /gene="ydgT"
                     /locus_tag="ACHJZ2_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO4569803.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            12345..12785
                     /locus_tag="ACHJZ2_003771"
     CDS             12345..12785
                     /locus_tag="ACHJZ2_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO4569804.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            12862..13443
                     /gene="rsxA"
                     /locus_tag="ACHJZ2_003772"
     CDS             12862..13443
                     /gene="rsxA"
                     /locus_tag="ACHJZ2_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO4569805.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            13443..14021
                     /gene="rsxB"
                     /locus_tag="ACHJZ2_003773"
     CDS             13443..14021
                     /gene="rsxB"
                     /locus_tag="ACHJZ2_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO4569806.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            14014..>15911
                     /gene="rsxC"
                     /locus_tag="ACHJZ2_003774"
     CDS             14014..>15911
                     /gene="rsxC"
                     /locus_tag="ACHJZ2_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO4569807.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKAR"
BASE COUNT         3790 a         4162 c         3971 g         3988 t
ORIGIN      
        1 tgctgcactt ttttacgatt atcaatttca accacgattt ctttgaattt tttatccgtg
       61 agcggataaa accagataat aacgaatgcc agtagcataa atccgcaagg tactaaggca
      121 attgatgtgc ggatgcccat aattacttcc ggcgtttgca cctgattggc gatatatccg
      181 cttaatccca aaataaaggc aggaattgaa ccaccgattg cctgaccgca tttacgggta
      241 aatgagaata gtgaataggt gagcccttca attcgcacgc cggtcaggta ttcaccgtat
      301 tctacggtat cagcttccag cgcccacatc acggtcatgg taacgccctg accaattgag
      361 gcaatggcta acgcaaccaa cgccaccggc agcgaccaga cggaaaccca gaagaacagc
      421 agataaccgc aggttcccag caaagcgcca atcaggaagg tattcttttt accgatcctc
      481 gcgaccatcc ccggcaccaa cggtgccgat gccacagtac caaccaggtt ttgcaccagt
      541 accagcacag tgaacagccc ggtatcattt aacacatagc gcacgtagaa caacgacgag
      601 gcgctgaccg cgaaggtcga aatcagcaca cacagcgcac cgatgcacaa cataaacagc
      661 gggcgattcc gtttcagggt ttgcagactg atcttcaatg acggctgcgc cacgatacgt
      721 accacattct cacgcgtcga tttgaagcag atgaagtaaa gcaccattcc ggcaatcgcc
      781 agcacaatcg tccagaaatg gtataccgac accatctctt ccgggctgga gttcttaatg
      841 ctcggcccta tcagaaatgc caggcagaca aaggtcaatg aagcggcaat cccacgagcc
      901 gcgcccagac gggcgcggga ttgtggttgt tgggtcatcg cggtagcaag tgaaccataa
      961 ggaatattca ccaggctgta gcaaagcccg aggcccatgt aggtcaaata tgcatacacc
     1021 actttgctac catggctcca gtcggtcggc acccagaata ccagcacgct gaagatcatt
     1081 aacggcgcag taccgaagag taaaaacggg cggaattttc cccagcgggt attcacactg
     1141 tccaccactc gtccggcaaa gacgtcggcg aaggcatcga atacccgcac cagtaacagc
     1201 atggtgcccg ccgcagcggc accgacgcca gcgacgtcgg tgtagtaact caacaggaag
     1261 agcgccccca ttgcgaaggc gaagttattg gcgatgtcac caaggctgta gccgacgatg
     1321 gtgcgccagg agagttgttg attcattgtt tgcctccctg ctgcggtttt tcaccgaagt
     1381 tcatgccagt ccagcgtttt tgcagcagaa aagccgccga cttcggtttg cggtcgcggg
     1441 tgaagatccc cttcttgtta ccgccaacgc gcaatatgcc ttgcgaggtc gcaaaatcgg
     1501 cgaaattcca tacctgttca ccgacgacgg cgctgacgcg atcaaagacg cggtgataca
     1561 tatccagcca tgcacactga tactcttcac tccacatgtc ggtgtacatt gagtgcagcc
     1621 cggctaacgt atccacgccg tattcggtga tgataatcgg ctgatgcagt ttctcctgcc
     1681 aggccagaag ttctttttcc agtaccttct ctgccgtttc caaatcgccg ctttggacat
     1741 accaaccgta ataacggttc aggcacagca catcaaagag atcgctgatg gtatcggtgt
     1801 gagcgtcgca gaacattaca ttgacgcagg tgatcggacg cgtcggatcg agtttacgcg
     1861 ttgcttccgc cagtggcgcg aaatattccc gtgcaccttg cggacgggta tccggttcgt
     1921 tggcaatact ccacatcacc acgcttgggt ggtttttgtc acgcgctatc agctctttaa
     1981 tcgcctgtaa gtgcgcctgc tgagtttccc cgttgactgc ctcttcgctg tacagttctt
     2041 tcggcttgtt gcccgcttcg aaaccaatgc ctaaagagag gttaaagccg acagctgcag
     2101 tttcatcaat caccacgatg ccatgttcat ctgcccagtc gagcatctct tcagcgtaag
     2161 ggtaatgcga ggtacggtag gagttggccc caatccagtc cattaatgcg tgatcgtgca
     2221 ccatcagcac gttatcgaat cctttgccgc gcaaatccgc atcttcatga cggccaaagc
     2281 cagtaaagta gaacggtttg tggttgatca ggaactgttc gcccttcact gccactgacc
     2341 ggatgccgac gcgcagcggg tagatatcac actctgtccg gcttttggct gtgacgtaca
     2401 gttcatagag ataaccttca ccaggttgcc agaggtgcgg attcaccact tgcaaagtcc
     2461 cgctggtgcc ccttgtccag ttgcaaccac ctgttgatcc gcatcacgca gttcaacgct
     2521 gacatcacca ttggccacca cctgccagtc aacagacgcg tggttacagt cttgcgcgac
     2581 atgcgtcacc acggtgatat cgtccaccca ggtgttcggc gtggtgtaga gcattacgct
     2641 gcgatggatc ccggcgtagt taaagaaatc atggaagtaa gactgctttt tcttgccgtt
     2701 ttcgtcggta atcaccattc ccggcgggat agtctgccag ttcagttcgt tgttcacaca
     2761 aactgtgata cgtacacttt tcccggcaat aacatacggc gtgacatcgg cttcaaatgg
     2821 cgtatagccg ccctgatgct ccatcacttc ctgattattg acccacactt tgccgtaatg
     2881 agtgaccgca tcgaaacgca gcacgatacg ctggcctgcc caacctttcg gtataaagac
     2941 ttcgcgctga taccagacgt tgcccacata attacgaata tctgcatcgg cgaactgatc
     3001 gttaaaactg cctggcacag caattgcccg gctttcttgt aacgcgcttt cccaccaacg
     3061 ctgctcaatt ccacagtttt cgcgatccag actgaatgcc cacaggccgt cgagtttttt
     3121 gatttcacgg gttggggttt ctacaggacg taacataagg gactcctcat taagataaaa
     3181 atactggtca acctttaatc tgattagtta accaattctg tgttgcacac agttatctgt
     3241 gagaaaccca tggaagagaa atggaaataa aaagaactga caccgcgatc aaaaaaccaa
     3301 aaactgcaat acccacagcg tgatatagat cgcattaatc tttaaaacgg cgttgacaga
     3361 agccataaaa taagcaaccg aacgttcgtt cgagcatgtt gggaacgata aactctcatg
     3421 acatatggct acggtgaata ttttagggag gaattccccg ctccggcgag attgtatttt
     3481 gtacagggaa attaaagtat gaaacgcggg ctcagttgtc accagcccgc ggaaagagag
     3541 agagaatcag gatgcggtta agataccgcc aatcatcgcg cgtaatccct gagcaaacga
     3601 cgcttcacgc gccaggtctt ccgcttcgat atcggccagt acaccgtagg tcagatccag
     3661 cagtaaacgt gaagtcattg ccgtgttgat gtctgggctt atctcgcctt tttgctgggc
     3721 atcacgcatc cgctgagcaa caaattcagt gatcgtcata tgcttatttt taagcatggc
     3781 ggcgacctgt gggttacgca tcccttccgc cataatttcg accaccagcg cccgttgccc
     3841 aaaggcttca tgggtgagag agacaataga ctcgaccata tagtcaacaa aatgaatccc
     3901 ttcaatcggt tcccggaaac gggccagcgc cctctcctgg tcctgtaaga taatggcctg
     3961 aatcaaggct tctttggaga tgaaatggtg atagagcgtc ccgggactaa tggcgcagga
     4021 tttacagatg gctttcatcg aggcactgtg aaatccattt tctgaaaaaa tctctctggc
     4081 agcattgagg atccgggtcc gtgtcggttg tgcttcagtc tgcatgttat ccatcattaa
     4141 accattatag ttagcgtgaa taactagttt accgcgttgt caggtgagcg cctatcattg
     4201 ctgttgaata gtgcgaaggc acactctatt cgtatgcttt tcatccagct attggttaac
     4261 caatggccgt gatttggctg ccagttgttt agcagagacg ttgatgaatt gcacacgcta
     4321 tcccggtaaa caacacatac ggctgtttac cggttcgtta gtgtattaat tgagctcctg
     4381 taccccacca ccggagacgg tgagaatttg tccgcttacc cagctggctg caggcgagca
     4441 aaggaacagc gctgcgttag caatatcttg cggttggccc agacgtctga tcggcgtgtg
     4501 ctgtaacatt ttttgttcaa tttctggtgt aataacggat ttcagggcat cggttaatat
     4561 tgcccccggc gcaatgccat tcacccgaat atttttttca cccaggtcaa atgccatatt
     4621 tctgaccaga tgactggccg cagctttaga tgatgcatag gaagtcatgt ttatattttt
     4681 attttctgcc gccatagaag tgatggtcag aataacgcca ccgccatttt tttccatttc
     4741 tggcgcaaca agttgtgaca gatggaaaaa agaaaacaca ttcagttcat aagcacggcg
     4801 aaaatccgcc attggcatat caaacggttt aggtccaccg ccaccggcgt tgttaaccag
     4861 aatatcaact ttacccagct tactgatagc aaagtctgcc agtgcagaga gttcctgttc
     4921 ggaagtaata tcacaacggc aggcaaatgc ctgaccaccc agttgttgaa tttcgtctac
     4981 aacatggtta gctgcgtcgg cgttaatatc actgaccacc acagatgcgc cagctgtcgc
     5041 gaatgtaatg gcgatttctt taccaatacc tgcacccgca cctgtgatga tggcgcattt
     5101 tccgtcgagt ctcaggttgt cagaattaaa cacataaacc tcctgtgaac ttcagtaaaa
     5161 ataaaaggca ccgcctgtgt gccacatttt ttatcattaa acttgaggaa aggacactac
     5221 caggtatagc agataaagag aggattattt cgccgcaatg agccgggcgg gaataataag
     5281 attgcgtgaa tgcgtctctt catgggtgat tttttgcatc atgcgatccg caagtgtggt
     5341 accaagttcc cgcgcaggcg tgctggccca ggtaacgggt atatcatcaa gtgtggttgg
     5401 tgtcgcatcg gtaaatgccg ccagcgaaac ctgttgctca aagtaacgat cgactccgct
     5461 ttccccgctt tgccgccctg ctttcagcaa accaaaccat gcccccatcg caatagtttc
     5521 gttatagcaa accacggcac tgatggtcgg gttatgacgt aaaagcgccg tgatagcttc
     5581 cgcggcttgc ttctggctgg aagtgcactc caacacccaa tcgctgtgaa acggcaggcc
     5641 aaattttagt agagttgcac aatagccccc cacccgttct gcacgggtta atgaggaact
     5701 ttgccctccc agccaggcga tccgctgatg cccattgcga atgagatgct ccgtcaacaa
     5761 ctgtgcagcc tgcatgttgt ccgggcgaac cgtatcaaca tcatcaagat aactggcacg
     5821 ggaagcgaaa atcaccggga tagctttttc ttctgccatc cgtcgcaggt catcgctgct
     5881 tcctgcagcc ccggcaatta ccacaccatc gacaccctga ttcagtaaca gtgaaaaccg
     5941 ctgtgccagc tgctcgccgt ctttaccgcc gtgaagcaaa aaaaccatcc gtccctgcgc
     6001 ttccagagct tccgtcaatc cggccgtcaa ttcggcgtaa aacggcgcag ataaatcacg
     6061 gacgatcaaa ccaatgacgc cgctttgccc accgcgcagc gccgacgcct ggcgattgcg
     6121 cacaaatccc agctcttcaa tggcggcgtt aacgcgttct cctgtggcgg tagagattcg
     6181 ccctttgcca ctaagcacca gcgaaacggt acttaccgac acgcccgcag ccagcgcaac
     6241 atcatgaatg gttatttttt tggcggtagc catgcagatg acctactccc tgattttgtg
     6301 acagataaaa cgttttacct tttatttatc ttatacccgc tattatcgtt gcgtaatgtg
     6361 atttatgcct cactaaaatt tgataaaacg ttttatcttc tcgcgcaatt tactgaatcc
     6421 agattgttct ctacgaggag tcgttttatg acggcgaaaa cagcaccgaa agtcacgctg
     6481 tgggagttct tccagcagtt aggcaaaacc ttcatgttac ccgtggcatt attgtcgttc
     6541 tgcggcatta tgctcggcat tggtagttcc cttagtagcc acgatgtcat caccctgctc
     6601 ccggtcctgg gcaaccccgt gttgcaggct atctttacct ggatgagtaa gattggctcg
     6661 tttgctttta gtttcctgcc agtcatgttc tgtatcgcca tcccgctggg tctggcacga
     6721 gaaaacaaag gcgtagcggc attcgctggc ttcgtcggtt atgcggtaat gaacctcgcg
     6781 gtaaacttct ggttgaccaa taaaggcatt ctgccaacca cggatgccgc cgttctgaaa
     6841 gccaataaca tccagagcat tcttgggatc cagtcgatcg acaccgggat cctcggtgcg
     6901 gtgatcgccg gtattatcgt ctggatgctg catgagcgat tccataatat ccgcctgccg
     6961 gatgcgctgg cattcttcgg cggtacgcgc ttcgtaccaa tcatctcctc gctggtgatg
     7021 ggccttgtcg gcctggtgat tccattagtc tggccgattt tcgccatggg tattagcggc
     7081 ttaggccata tgatcaatag cgcgggtgat ttcggaccga tgctgtttgg taccggtgaa
     7141 cgtctgctgt tgccgtttgg tctgcatcac attctggtgg cattaattcg ctttaccgac
     7201 gcaggtggca cgcaggaagt ctgcggtcaa accgtcagcg gcgcattgac catcttccag
     7261 gcgcaattga gttgcccgac cactcacggt ttttctgaaa gcgccacgcg tttcctttcg
     7321 caaggcaaaa tgcctgcgtt tctcggcggt ctgccaggtg cagcgttagc gatgtatcac
     7381 tgcgcgcgcc cggaaaatcg ccataaaatt aaagggctgc tgatttctgg cctgatcgcc
     7441 tgtgtcgttg gcggcacgac cgaaccgctg gaattcctgt tcctgttcgt agcgccagtt
     7501 ctgtatgtca tccacgcgct gttaaccggc ctcggcttca ccgtcatgtc tgtgctcggc
     7561 gtcaccatcg gtaataccga cggcaatatc atcgacttcg tggtgttcgg tattttgcat
     7621 ggtctgtcaa ccaagtggta catggtgcca gtggtggtgg caatctggtt tgtcgtttac
     7681 tacgtcatct tccgtttcgc tatcacccgc ttcaatctga aaaccccggg acgcgatagc
     7741 gaagtggcca gttcaatcga aaaagccgtt gccggtgcgc cgggtaaatc aggttacaac
     7801 gttccggcaa tcctcgaagc cttaggcggt gccgacaata ttgttagcct cgataactgc
     7861 attacccgtc tgcgtttgtc tgtgaaagat atgtcgcttg ttaatgtgca ggcactgaag
     7921 gacaatcggg caattggcgt agtacaactt aatcaacata acctgcaggt tgttatcggg
     7981 ccacaagttc agtcagtaaa agatgaaatg gccggtctga tgcatactgt ccaggcataa
     8041 ggataagata tgttcgattt ttcaaaggtc gtggatcgtc atggcacatg gtgtacgcag
     8101 tgggattatg tcgctgaccg tttcggcact gctgacctgt tacctttcac gatttcagac
     8161 atggattttg ccactgcccc ctgcattatc gaggcgctga atcagcgcct gatgcacggc
     8221 gtgtttggct acagccgctg gaagaacgat gagtttctcg cggctattgc ccactggttt
     8281 tccacccagc attacaccgc catcgatcct cagtcggtgg tgtatggccc ttctgttatc
     8341 tatatggtct cagaactgat tcgtcagtgg tctgaaacag gtgaaggcgt ggtgatccac
     8401 acacccgcct atgacgcatt ttacaaggcc attgaaggta atcagcgcac agtaatgccc
     8461 gttgctttag agaagcaggc tgacggctgg ttttgcgata tgggcaagct ggaagccgtg
     8521 ttggcgaaac cagaatgtaa aattatgctc ctgtgtagcc cacagaatcc taccggaaaa
     8581 gtgtggacgt gcgatgagct ggagatcatg gctgacctgt gcgagcgtca tggtgtgcgg
     8641 gttatttccg atgaaatcca tatggatatg gtttggggcg agcagccgca tattccctgg
     8701 agtaatgtgg cgcggggaga ctgggcgttg ctgacgtcgg gatcgaaaag tttcaatatt
     8761 cccgcgctga ccggtgctta cgggattata gaaaatagca gcagccgtga tgcctattta
     8821 tcggcactga aaggacgtga tgggctttct tccccttcgg tactggcgtt aactgcccat
     8881 atcgccgcct atcagcaagg tgcaccgtgg ctggatgcct tacgcgtcta tctgaaagat
     8941 aacctgacgt atatcgcaga taaaatgaac gccgcgtttc ctgaactcaa ctggcagatc
     9001 ccgcaatcca cttatctggc ctggcttgat ttacgtccgt tgaatattga cgacaacgcg
     9061 ttgcaaaaag cacttatcga acaagaaaaa gtcgcgatca tgccggggta tacctacggt
     9121 gaagaaggtc gtggttttgt ccgactcaat gccggctgcc cacgttcgaa actggaaaaa
     9181 ggtgtggctg gattaattaa cgccatccgc gctgttcgct aaccccaatt gcgcaacgta
     9241 aaaaatcgtt gcgcaatcgt ggatttttac cctgctttgt ttttataatg gtgcgcactt
     9301 ttatatccag aaaaagagtg cgaccatgat tgataccacc ctgccattaa ctgatatcca
     9361 tcgccacctt gatggcaaca ttcgtcccca gaccattctt gaacttggcc gccagtataa
     9421 tatctcgctt cctgcacaat ccctggaaac actgattccc cacgttcagg tcattgccaa
     9481 cgaacccgat ctggtgagct ttctgaccaa acttgactgg ggcgttaaag ttctcgcctc
     9541 tcttgatgct tgtcgccgcg tggcatttga aaacattgaa gatgcagccc gtaacggcct
     9601 gcactatgtc gagctgcgtt tttcaccagg ctacatggca atggcacatc agctgcctgt
     9661 tgcaggtgtt gttgaagcgg tgatcgatgg cgtacgtgaa ggttgccgca cctttggtgt
     9721 gcaggcgaag cttatcggca ttatgagccg aaccttcggc gaagccgcct gtcagcaaga
     9781 gctggaggcc tttttagccc accgtgacca gattaccgca cttgatttag ccggtgatga
     9841 acttggtttc ccgggaagtc tgtttctttc tcacttcaac cgcgcgcgtg atgcgggctg
     9901 gcatattacc gtccatgcag gcgaagctgc cgggccggaa agcatctggc aggcgattcg
     9961 tgaactgggg gcagagcgca ttggacatgg cgtaaaagcc attgaagatc gggcgctgat
    10021 ggattttctc gccgagcaac aaattggtat tgaatcctgt ctgacctcca atattcagac
    10081 cagcaccgtg gcagagctgg ctgcacatcc gctgaaaacg ttccttgagc atggcattcg
    10141 tgccagcatt aacactgacg atcccggcgt acagggagtg gatatcattc acgaatatac
    10201 cgttgccgcg ccagctgctg ggttatcccg cgagcaaatc cgccaggcac agattaatgg
    10261 tctggaaatg gctttcctca acgcagagga aaaacgcgca ctgcgagaaa aagtcgcagc
    10321 gaagtaacaa aaatggatgg tgcaaattgc accatccatt tttcatgcaa ggcacaaagt
    10381 cgcgcgatgt ttggcggatt cgatgcccag ctcaatcaac tccattacct ggattgcctg
    10441 gcttgccgga accggatttt caccatcgcc atttaacgca tcacgaatcg ccgcatagta
    10501 agccggataa ttcccaggca ccgtcaacag cgtttcttcg acacgttcct caccttccac
    10561 gcgggtaagt acgccatcac gcatatcgta gccccagtct tcctgcggca gacgctcgcc
    10621 atttttcaga cgttcttcct gtggatcgag gccatatttc acataactgc ctcgggatcc
    10681 atgcacgata taccgtgctg actcagcggc tgccagcatg gtaccgtgta aaatgactcg
    10741 ccgctgtgga taggacaaga tggcgtggaa ataatcggtc gactgcgctc cgggccgtaa
    10801 ctgtgccaaa tctaccgtca tgctgaccgg taaaccaaat agcgtaatgg cctgatcaag
    10861 aagatgtggt gctaaatcgt accagatacc gctgcctggg ccgccctgtt cacgccaacg
    10921 atcgcgcacc tgcggacgga agcggtcaaa atgagactca aagtaagcaa cttcacccag
    10981 cacgccttcc gcgagcaaac cttttagcgt caagaaatcg ctatcccaac gacggttatg
    11041 gaatacagac agaacacgcc ccaggctttt tgccagcgca tccagctctc gcgcttgtga
    11101 cagtgtcacg gtaaagggtt tatcaacgac cacatgttta cccgcctcaa gcgctgcttt
    11161 ggctaacggg aaatgggtat cgttgggtgt aggaatgaca atcaggtcta tgttgggatc
    11221 gttaaacaga tgcttcggct cagagacaac cgtaaccgtt ggccagtcgg cttttacttt
    11281 tgtttcatca ctgctggaga ttaccgccag ttccagcccg ggtgtgcccg caatcagggg
    11341 cgcatggaag gttttgctcg cataaccata cccaatcaac ccaacacgga tgttgtcgct
    11401 catgtcattg cctctcattg aagtatgatg gctatttgac actatccttt accctcgctc
    11461 aacagtttaa taacctgcca gcaataaggg atgttgttta acttaagtca aaaaaatagc
    11521 gatttttcca acgacaaaag ctaaatatcg caaaaacctc agtaaaaatc ttgctggagc
    11581 tattattgct aagtaacatt taccccctga agttaatgga tcaatcaaca gagatgtgag
    11641 ttgtaatgaa tcgtcttatt gaattaacag gttggatcgt tcttgtcgtt tcagtcattc
    11701 ttcttggcgt ggcgagtcac attgacaact atcagccacc tgaacagagt actacggtac
    11761 aacacaagta agccctgcgc ttgtagagcg acatgctgcc cgtcagggtg catgttttca
    11821 cttgtcggat attaaaccag gaatttatta tcttgttcgg tgttgttggt gattgtcagg
    11881 gatagtaaag cgcgttactc ttttcatagc agtttttcat ccctgaatat ttgccatcag
    11941 cgcattctta ttattgtaat aacacaagcg ttcacttttg gtgaaatttt atcatcacta
    12001 ataataccta cccctttggt attaattatt ttatggacct tttatgactg ttcaggacta
    12061 cttattaaaa tttcgcaaaa tcagttcact cgaaagtctg gaaaaactct acgaccatct
    12121 taattacacc ctgacggacg atcaggaact gatcaatatg tatcgtgctg ccgatcaccg
    12181 tcgcgcagag ctggtttctg gcgggcgttt gtttgacctc ggccaggtac cgaagtccgt
    12241 ctggcactat gttcaataaa gaaagtagcg atcggttata aaagccttat aataacgccc
    12301 ctaaaattag atttgcctgt ttgcgtcgct attctggaga acctatgacc accacaacgc
    12361 cacaacgtat tggaggctgg ttgcttggcc cgttggcctg gcttttagtc gctctgttaa
    12421 gtacgacgct ggcgttgttg ctgtataccg ctgcgttatc ttctccgcaa acatttcaaa
    12481 cgctcggcgc acaagcactg acgacacaaa tcttatgggg cgtttctttt attaccgcta
    12541 tcgccatgtg gtattacaca ctgtggttga ccatcgcttt ctttaaacgt cggcgttgtg
    12601 ttcctaaaca ctatattatc tggctgctta tttccgtatt gctggcggta aaagcgttcg
    12661 ctttttcacc cgtggaagat ggcatagccg ttcgccagtt gctgtttact ttgttggcaa
    12721 ccgcattgat cgtcccctat ttcaaacgct cgtcgcgggt gaaagcgacg tttgtgaatc
    12781 cgtaataacc ttacagttaa cctgttgtcg cctgctctgg attaacggat aataggcggc
    12841 ttttttattt caggccgaaa aatgactgac tacctgttac tgtttgtcgg aactgtactg
    12901 gtcaataact ttgtactggt caagtttctc ggtctctgtc cgtttatggg ggtttccaaa
    12961 aagctggaaa ccgcgatggg catggggctg gcaacaacgt ttgtgatgac gctggcgtct
    13021 atttgcgcct ggcttatcga tacgtggatt ttgatcccac ttaatctgat ttacctgcgc
    13081 accctggcat ttattctggt gattgctgtg gtcgtgcagt tcaccgagat ggtggtgcgc
    13141 aaaaccagcc cggtgcttta ccgtttgctg gggatttttt tgccgcttat caccaccaac
    13201 tgtgccgtgc tcggcgtggc gttgctgaat atcaatctcg ggcacaattt cttgcagtcg
    13261 gcgctgtacg gtttttccgc cgctgtcggt ttttcgctgg tgatggttct cttcgcagcc
    13321 atccgcgaac gccttgctgt ggctgatgtc ccggcacctt ttcgcggtaa tgccattgcg
    13381 ttaattaccg caggtcttat gtctctggcc tttatgggct ttagtggttt ggtgaagttg
    13441 taatgaatgc tatctggatt gccgttgccg ccgtgagcct gctgggcctg gcgtttggcg
    13501 ccattctggg ttatgcctcc cgccgttttg cggtggaaga cgatccggtc gttgagaaaa
    13561 ttgacgaaat cttaccgcag agccagtgtg gtcagtgcgg ttatcccggc tgtcgcccct
    13621 acgcggaagc catcagctgt aacggtgaaa aaatcaaccg ttgcgcccct ggtggcgaag
    13681 ctgtgatgct aaaaattgcc gagttgctta atgtcgagcc gcagccgctg gatggcgaag
    13741 cgcaagagct aacgcctgcg cggatggtgg cggttattga tgaaaataac tgtattggct
    13801 gcactaaatg tattcaggcg tgtccggtag acgccatcgt tggcgctacc cgtgccatgc
    13861 atacggtaat gagtgatctc tgtacgggct gcaatttatg tgttgatccg tgcccgacgc
    13921 actgcatctc gttgcaaccg gtcgcagaaa cacctgactc ctggaaatgg gatctgaaca
    13981 ccattcccgt gcgtatcatt cccgtggaac accatgctta agttattctc tgcattcaga
    14041 aaaaataaaa tctgggattt caacggcggc atccatccac cggagatgaa aacccagtcc
    14101 aacggtacac ccctgcgcca ggtaccactg gcgcagcgtt ttgtcattcc actgaaacag
    14161 catattggcg ctgaaggtga gttgtgcgtt agcgtcggcg ataaagtatt gcgcggccag
    14221 ccgcttaccc gtggtcgcgg caaaatgctg cctgttcacg cgcccacctc gggtaccgtt
    14281 acggctattg cgccccactc tacggctcat ccttcagctt tagctgaatt aagcgtgatt
    14341 attgatgccg atggtgaaga ctgctggatc ccacgagacg gctgggccga ttatcgctct
    14401 cgccgtcgcg aagagttaat cgagcgcatt catcagtttg gtgttgccgg gctgggtggt
    14461 gcaggattcc cgacaggcgt taaattgcag ggcggcggag ataagattga gacgttgatt
    14521 atcaacgctg ctgagtgcga gccatacatt accgccgatg accgtttgat gcaggattgc
    14581 gcggctcagg tcgtagaggg tattcgcatt cttgcgcata ttctgcagcc acgcgaaatt
    14641 cttatcggca ttgaagataa caaaccgcag gcgatttcca tgctgcgcgc ggtgctggcg
    14701 gactctcacg atatttctat gcgggtgatt ccaaccaaat atccttctgg cggtgctaaa
    14761 caattaacct acattctgac cgggaagcag gttccacatg gcgggcgttc atccgatatc
    14821 ggcgtattaa tgcaaaacgt cggcactgct tatgcagtga aacgtgccgt tattgatggc
    14881 gagccgatta ccgagcgtgt cgtaaccctg actggcgaag caatcgctcg cccgggcaac
    14941 gtctgggcac ggctggggac gccagtgcgt catttattga atgacgccgg attctgcccc
    15001 tctgccgatc aaatggtgat tatgggcggc ccgctaatgg gctttacctt gccatggctg
    15061 gatgtcccgg tcgtaaagat taccaactgt ctgttggctc cctctgccaa tgagcttggc
    15121 gaaccacagg aagaacaaag ctgcatccgg tgtagcgcct gtgctgacgc ctgccctgcg
    15181 gatcttttgc cgcaacagtt gtactggttc agcaaaggtc agcaacacga taaagctacc
    15241 acgcataaca ttgccgactg cattgaatgt ggggcttgcg cgtgggtttg cccgagcaat
    15301 attcccctgg tgcaatattt ccgtcaggaa aaagctgaaa ttgcggctat tcgtcaggaa
    15361 gaaaagcgcg ccgcagaagc caaagcgcgt ttcgaagcgc gccaggctcg tctggagcgc
    15421 gaaaaagcgg ctcgccttga acgacataag agcgcagccg ttcaacctgc agccaaagat
    15481 aaagatgcga ttgctgccgc tctggcgcgg gtgaaagaga aacaggccca ggctacacag
    15541 cctattgtga ttaaagcggg cgaacgcccg gataacagtg caattattgc agcacgggaa
    15601 gcccgtaaag cgcaagccag agcgaaacag gcagaactgc agcaaactaa cgacgcagca
    15661 accgttactg atccacgtaa aactgccgtt gaagcagcta tcgcccgcgc caaagcgcgc
    15721 aagctggaac agcaacaggc aaatgcggaa ccagaacaac aggtcgatcc gcgcaaagct
    15781 gccgtcgaag ccgctattgc ccgtgccaaa gcacgcaagc tggaacagca acaggctaat
    15841 gcggaaccag aacaacaggt cgatccgcgc aaagccgccg tcgaagccgc tatcgcccgc
    15901 gccaaagcgc g
//
LOCUS       DAZFBR010000107        15722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFBR010000107 DAZFBR010000000
VERSION     DAZFBR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.107"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            42..383
                     /gene="yiaB"
                     /locus_tag="ACHJZ2_003775"
     CDS             42..383
                     /gene="yiaB"
                     /locus_tag="ACHJZ2_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO4569808.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            429..866
                     /gene="yiaA"
                     /locus_tag="ACHJZ2_003776"
     CDS             429..866
                     /gene="yiaA"
                     /locus_tag="ACHJZ2_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO4569809.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(908..1903)
                     /gene="wecH"
                     /locus_tag="ACHJZ2_003777"
     CDS             complement(908..1903)
                     /gene="wecH"
                     /locus_tag="ACHJZ2_003777"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO4569810.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYPYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            2078..2377
                     /gene="ysaB"
                     /locus_tag="ACHJZ2_003778"
     CDS             2078..2377
                     /gene="ysaB"
                     /locus_tag="ACHJZ2_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO4569811.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2472..3383
                     /gene="glyQ"
                     /locus_tag="ACHJZ2_003779"
     CDS             2472..3383
                     /gene="glyQ"
                     /locus_tag="ACHJZ2_003779"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO4569812.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3393..5462
                     /gene="glyS"
                     /locus_tag="ACHJZ2_003780"
     CDS             3393..5462
                     /gene="glyS"
                     /locus_tag="ACHJZ2_003780"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO4569813.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            5785..5937
                     /gene="hokA"
                     /locus_tag="ACHJZ2_003781"
     CDS             5785..5937
                     /gene="hokA"
                     /locus_tag="ACHJZ2_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO4569814.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(5914..5985)
                     /locus_tag="ACHJZ2_003782"
     CDS             complement(5914..5985)
                     /locus_tag="ACHJZ2_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569815.1"
                     /translation="MRNAVRKAGITFRRRLLLFQIAG"
     gene            complement(6125..6337)
                     /gene="cspA"
                     /locus_tag="ACHJZ2_003783"
     CDS             complement(6125..6337)
                     /gene="cspA"
                     /locus_tag="ACHJZ2_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO4569816.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(6618..6908)
                     /gene="yiaG"
                     /locus_tag="ACHJZ2_003784"
     CDS             complement(6618..6908)
                     /gene="yiaG"
                     /locus_tag="ACHJZ2_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO4569817.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            7342..8052
                     /gene="yiaF"
                     /locus_tag="ACHJZ2_003785"
     CDS             7342..8052
                     /gene="yiaF"
                     /locus_tag="ACHJZ2_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO4569818.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(8102..9076)
                     /gene="ghrB"
                     /locus_tag="ACHJZ2_003786"
     CDS             complement(8102..9076)
                     /gene="ghrB"
                     /locus_tag="ACHJZ2_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO4569819.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(9180..9839)
                     /gene="yiaD"
                     /locus_tag="ACHJZ2_003787"
     CDS             complement(9180..9839)
                     /gene="yiaD"
                     /locus_tag="ACHJZ2_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO4569820.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVNVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            9992..12325
                     /gene="bisC"
                     /locus_tag="ACHJZ2_003788"
     CDS             9992..12325
                     /gene="bisC"
                     /locus_tag="ACHJZ2_003788"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO4569821.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(12294..12734)
                     /gene="yiaC"
                     /locus_tag="ACHJZ2_003789"
     CDS             complement(12294..12734)
                     /gene="yiaC"
                     /locus_tag="ACHJZ2_003789"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4569822.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(12731..13294)
                     /gene="tag"
                     /locus_tag="ACHJZ2_003790"
     CDS             complement(12731..13294)
                     /gene="tag"
                     /locus_tag="ACHJZ2_003790"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO4569823.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            13452..14150
                     /gene="yhjY"
                     /locus_tag="ACHJZ2_003791"
     CDS             13452..14150
                     /gene="yhjY"
                     /locus_tag="ACHJZ2_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO4569824.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            14379..15581
                     /gene="yhjX"
                     /locus_tag="ACHJZ2_003792"
     CDS             14379..15581
                     /gene="yhjX"
                     /locus_tag="ACHJZ2_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4569825.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVKFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         3864 a         3806 c         4025 g         4027 t
ORIGIN      
        1 tggttactcg taatgtatcg ctggtagata tggagatcgt tatgaaaacc tcaaaaactg
       61 tggcaaaact attatttgtt gtcggggcgc tggtttatct ggttgggcta tggatctcat
      121 gcccattgtt gagtggaaaa ggctattttc ttggcgtgtt aatgacagca acttttggca
      181 actatgcata tcttcgcgca gaaaaactcg ggcaactgga taattttttt acccatattt
      241 gccagttagt tgcgttaatc actatcggtc tcttgtttat cggtgtttta aatgcaccta
      301 tcaatgctta tgaaatggtg atctacccca tcgccttttt tgtctgcttg tttggtcaaa
      361 tgcgtttgtt tcgctcggta tgagcaacat aaagctctta cacattcagg aatgaaagga
      421 atactgtgat ggacaacaaa atatcaacct attcaccggc ctttagtatt gtgtcatgga
      481 tagctctcgt tggtggtatc gttacctatc tgttagggct atggaatgca gagatgcagt
      541 taaatgaaaa aggatattat tttgccgtac tggtattagg actgttttct gcggcgtctt
      601 atcaaaagac cgttcgggac aagtatgaag gcataccgac cacttccatt tattatatga
      661 cctgcctgac tgtctttatt atctctgttg cattactgat ggtaggtctg tggaatgcga
      721 cattattact cagcgaaaaa ggtttttatg gactggcttt cttcttaagc ttgtttggtg
      781 cagtagcggt gcagaaaaat attcgtgatg ccggaataaa cccaccaaaa gaaacacagg
      841 ttacccagga agaatacagc gaataactca cgtaagcccg gtcagtccaa tgtgaccggg
      901 cttttactta actcactaat ctgtttctgt cgattcgttg taccagcata gaaagtaaca
      961 aactcgctgc caacgtcgcg caaaaaatcc aaataatatc cagtattggc caatttttaa
     1021 gctcaattcc ccgggtgcgc agcgcatgga taatcaaggc gtggaatccg tatataccca
     1081 acgaatggcg ggagattaag ccaagtccgc gaatggtacg cgtatccagc gtgtttttaa
     1141 ccagagtcaa tagcgcgatt gcgcagataa aaaccatcgg cccacagtaa ggataccagg
     1201 tatcggcaaa atttccgcgc cactgcaatt catataatgt cccgcgagag ataataaaaa
     1261 ctcccgtcgc aaacagtgcg gcgctcaccc acgacagtgc tttatgctgt gtgtccatca
     1321 tccctaaagc gcggcccaac atgccataca gaatgtagta aaaagtatcg ccattgatat
     1381 ataagttaat tggcagccat tcaaaaccgt cgattttctg cggcacggtg tttgggttag
     1441 cgatgatacc aatcaccacc attagcacca gcaacatttt tccgccgacg ttcttcacct
     1501 gaatcagcgg tgaaaccaga taaatcaccg caatcgcgaa gaaaaaccat aagtggtaaa
     1561 acactggctt ttgcagcagg tttttcagcg ctaactccac attaatggag gtaaacagtg
     1621 caatgtagag cagcgcgatt gcgctataaa aaaacagaca taagccgata cgcaagaaat
     1681 ggcgcggctg ggcgctgcgt tcgccaaaaa agagatagcc ggaaatcatg aaaaatagcg
     1741 gcacgctgac acgagaggca gagttcagaa cattggcgat atcccaggtg acggggctaa
     1801 cactatgagc attggtcaca taccaggtag tggtgtgaat catcaccacc attaaacacg
     1861 ctatccctcg caggttatca atccagtaaa ttttgggctg catctgtgtc tctgtatctg
     1921 gttaaaaaaa gtctgaccga taaatcattc agttggcgca ctggaataat ctgagtttta
     1981 acactacagc ttatagaggc ttaaggaaat tcgtaagata tcagccacta taccgatata
     2041 aataataaga ctcacctgca aaccagacgg taatttaatg atgatgaact ctttctttcc
     2101 ggcaatggcg cttatggtgc tagtgggttg ttctacaccg tcacccgtgc agaaagcaca
     2161 acgggtaaag gttgatcctc tgcgttcgtt gaatatggaa gcgttatgca aggatcaggc
     2221 ggcaaaacgt tataacaccg gcgagcaaaa aatcgacgtc accgccttcg aacagttcca
     2281 gggaagctat gaaatgcgcg gttatacctt ccgtaaagag cagtttgtct gttcttttga
     2341 cgcagatggc cattttttgc atctttccat gcgttaagcc ctgctttttc ccgtttcata
     2401 ctgtatatct tccatccagc gggtatactg atcccttcct ttaaatccac acgtatccag
     2461 cacgaaataa tatgcaaaag tttgatacca ggaccttcca gggcttgatc ctgaccttac
     2521 aggattactg ggctcgccag ggctgcacca ttgttcaacc attggacatg gaagtcggcg
     2581 cgggaacctc tcacccaatg acctgtctgc gcgcgctggg gccagaaccg atggcggctg
     2641 cttatgttca gccttctcgt cgcccgaccg atggtcgcta cggcgaaaac cccaaccgtt
     2701 tacagcacta ctatcagttc caggtggtca ttaagccatc gccggacaat attcaggagc
     2761 tgtaccttgg ttctctgaaa gagctgggca tggacccgac tatccacgat atccgtttcg
     2821 tggaagataa ctgggaaaac ccgacgctgg gtgcctgggg actgggctgg gaagtgtggc
     2881 taaacggcat ggaagtgacg cagttcactt acttccagca ggttggtggt ctggagtgta
     2941 aaccggttac cggcgagatc acctacggtc tggaacgtct ggccatgtac attcagggcg
     3001 tagacagcgt ttacgacctg gtctggagcg acggcccgct gggtaaaacc acctacggcg
     3061 acgtgttcca tcagaacgaa gtggagcagt ccacttacaa cttcgaatac gcggatgtgg
     3121 acttcctgtt cacctgcttt gagcagtacg agaaagaagc gcagcagctg ctggcgctgg
     3181 aaaatccgct gccgctgcca gcttacgagc gtattctgaa agccgcccac agcttcaacc
     3241 tgctggatgc gcgtaaagcc atctccgtca ccgagcgtca gcgctacatt ctgcgcattc
     3301 gcaccctgac caaagcagtg gcagaagcat attacgcttc ccgtgaagcc ctaggcttcc
     3361 cgatgtgcaa caaagataag taagaggcgg ctatgtctga gaaaactttt ctggtggaaa
     3421 tcggcactga agagctgcca ccaaaagcac tgcgcagcct ggcagagtcc tttgctgcga
     3481 actttactgc ggagctggat aacgcaggcc tcgcacacgg caccgttcaa tggtttgctg
     3541 ctccgcgtcg tctggcgctg aaagtagcta acctggcgga agcgcaaccg gatcgtgaaa
     3601 tcgaaaaacg cggcccggcg attgcccagg cgttcgacgc tgaaggcaaa ccgagcaaag
     3661 cggcagaagg ttgggcgcgt ggttgcggta ttaccgttga ccaggctgag cgtctgacta
     3721 ccgataaagg cgaatggctg ctgtatcgcg cccatgtgaa gggcgaaagc actgaagcac
     3781 tgctgccgaa tatggttgcg acttcgctgg cgaaactgcc gattccgaaa ctgatgcgct
     3841 ggggtgcaag cgacgtgcac ttcgtgcgtc cggtgcacac cgtgaccctg ctgctgggcg
     3901 acaaagtcat tcctgcaacc attctgggca ttcagtccga tcgcgtgatt cgcggccacc
     3961 gctttatggg cgagccggaa ttcaccatcg ataacgccga tcagtatccg gaaattctgc
     4021 gtgagcgtgg gaaagtcatc gccgattacg aagaacgtaa ggcgaagatt aaagccgatg
     4081 ccgaagaagc agcgcgtaag attggcggta acgctgactt aagcgaaagc ctgctggaag
     4141 aagtggcttc gctggtggag tggccggtcg ttctaaccgc gaaatttgaa gagaaattcc
     4201 tcgcggtgcc gtctgaagca ctggtttaca ccatgaaagg tgaccagaag tacttcccgg
     4261 tgtatgcgaa cgacggcaaa ctgctgccga actttatctt cgttgccaac atcgaatcga
     4321 aagatccgca gcaaattatc tctggtaacg agaaagtcgt tcgtccacgt ctggctgatg
     4381 ccgagttctt cttcaacacc gaccgtaaaa aacgtctgga agataacctg ccgcgcctgc
     4441 aaaccgtgct gttccagcaa cagctgggta cgctgcgtga caagactgac cgcatccagg
     4501 cgctggctgg ctggatcgcc gaacagattg gcgctgacgt taaccacgca acccgtgcgg
     4561 gcctgctgtc taagtgtgac ctgatgacca acatggtctt cgagttcacc gacacccaag
     4621 gcgttatggg gatgcactac gcgcgtcacg atggcgaagc ggaagacgta gccgtcgcgc
     4681 tgaatgagca gtatcagccg cgctttgccg gtgatgacct gccgtccaac ccggtagctt
     4741 gtgcgctggc gattgctgat aagatggata ctctggcggg tatcttcggt atcggtcagc
     4801 atccgaaagg cgacaaagac ccgtttgcgc tgcgtcgtgc cgcacttggc gtgctgcgaa
     4861 ttatcgttga gaagaacctc aaccttgatc tgcaaacgct gaccgaagaa gcggtgcgtc
     4921 tgtatggcga taagctgact aatgccaacg tagttgatga tgttatcgac tttatgctcg
     4981 gtcgcttccg cgcctggtat caggacgaag gttataccgt tgacaccatc caggcggtac
     5041 tggcgcgtcg tccgactcgt ccggctgatt tcgatgcccg catgaaagcg gtatcgcact
     5101 tccgtaccct ggaagcagct gcagcactgg cggcggcgaa caaacgtgta tctaacatcc
     5161 tggcgaaatc tgacgaagtg ctgagcgatc gcgtgaatgc ctctactctg aaagagccgg
     5221 aagaaattaa actggcgatg caggttgtgg tgctacgtga caagctggag ccgtactttg
     5281 cggaaggtcg ttaccaggat gcgctggtcg aactggcaga gctgcgtgaa ccagtcgatg
     5341 ccttcttcga taaagtgatg gtcatggttg atgacaaaga attgcgtatc aaccgtctga
     5401 ccatgctgga gaaactgcgc gaactgttcc tgcgcgttgc ggatatttcg ctgttgcagt
     5461 aataacgccg ttattaaaaa gcctgccatc tggcaggctt tttttatcgc taaataatac
     5521 agcaaccttt aataatcttc tgctgaataa agattatctc atatattaat tttatgagat
     5581 ttttttagga ttatatcaag gagaagaaac aaacttatta agctagaata gccacgggtg
     5641 cttgagactg tttgtctcag gtattcaccg aaaggcagac agagaaaagc cccacctgac
     5701 tataaatcaa agtgaggccc ctctatgtat agacaccata aggttagcct cttactcgtt
     5761 ggaaatcaac acagggggct gggaatgccg cagaaatata gattgctttc tttaatagtg
     5821 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
     5881 attaaacagg ggagttatga gctggcggca tttttagcct gcaatttgaa agagtaagag
     5941 tcttcggcgg aaagttattc ccgcctttct tacggcgttg cgcattctca ttgcacccaa
     6001 atttattttt cacaaaaata atactagatt ttattacgcg atcgattatt tatttcctga
     6061 aaaacaaata aaaaaatccc cgccaaatgg cagggatctt agattctgtg cttttaagca
     6121 gagattacag gctggttacg ttgccagctg ccgggccttt agcgccgctt tcgatggtga
     6181 aggacacttt ctgaccttcg tccagagatt tgtaaccatc gttctggata gcagagaagt
     6241 gtacgaacac gtctttagag ccatcgtcag gagtgatgaa gccgaagcct ttgtcagcgt
     6301 tgaaccattt tacgatacca gtcattttac cggacatagt gtattacctt taataattaa
     6361 gtgtgccttt cggcgatatg gcgtgcttta cagattttga agcgttaaag gaatgtgcac
     6421 tacgaggggt atcaacgata actcttgaag ggacttgcct tactacactg ctttaatggt
     6481 ctgtacgtca aaccgttgat gtgcattaag ccacgcattg gcgggtgatg caacaattat
     6541 ttttcatatt tatgattaat cggttggaaa acggtcctgt catcaggacc gtaaacagca
     6601 ataaagtgga taaaagtcta ttccatcaac tgcttactta atgccgggtt ggcttgaatc
     6661 aaacgcatca attttagttc ggcacttgaa ggcttcacgc gtctggattc ccattccttt
     6721 accatggcga ctgatacgcc caaaacccgg gcgaaatcat cgatttttaa tcctgtccct
     6781 tttcgtaact gctcaatttc ggtacaggac gttgttctgt gcgtcagggt aattttctgc
     6841 gtttcatctt taaaaacaat ctgttccagg ctgctcaaca gctcatgcat tggatcttta
     6901 tattccattg aaaactcctc aaatcacact gcgggatcgt gaattgcatc gaagctcatt
     6961 aagaatagtc gggaaaagca gacacagcgg ggtgaagtca gggcatgctc tggctatgat
     7021 tcgattcagc cagtcattta gcgtgactta tagccaccta acgatctgat aaatgccgat
     7081 atatctctaa ccactcctgg tttgtaaaga tttcgaaaat ctaaccttct gcgaaggcgt
     7141 aaatctctgg ctacaaaaaa ggcaacggca atatcatttt ttaacaatga aacaacaaat
     7201 taaccttgtt tcattttctc catgatttta ccaggaagat tacgtagaaa gggtatcctg
     7261 caagcctgtc ctggactatc cttgtcacgt cagacacgcg tgtgtcgttg tgcgcttttt
     7321 ttgggtgaaa ggagtaagaa aatggcgaca ggaaagtcct gctctcgctg gtttgcgcct
     7381 cttgcggcgt tattaatggt agttagcctg agtgggtgtt ttgataaaga aggcgatcag
     7441 cgtaaagcgt ttatcgactt cctgcagaat acagtgatgc gtagcggtga acgtctacca
     7501 accctgactg ccgatcagaa aaaacagttt ggtccttttg tctctgatta cgcgattctg
     7561 tatggttatt ctcagcaggt gaatcaggcg atggattccg gtctgcgccc ggttgtagac
     7621 agcgttaacg caattcgcgt accgcaggat tacgttacgc aaagcggtcc gctgcgtgaa
     7681 atgaatggtt cgctgggtgt gctggcgcaa caactgcaaa atgcgaagtt gcaggccgat
     7741 gctgcgcact ctgcgctaaa acagagtgat gacctgaaac cggtctttga tcaggcattc
     7801 accaaagtgg taacgacgcc agctgatgca ttgcaaccgt tgattccggc ggcacaaact
     7861 ttcacgcaac aactggtgat ggttggagac tacattgccc aacaaggtac tcaggtgagc
     7921 tttgtagcaa atggcatcca gttcccgact tcacagcagg caagtgaata taacaaactg
     7981 attgcgccat taccggcaca acatcaggcg tttaatcagg cctggactac agcagttact
     8041 gcaacccaat aaagagtaaa agcccgagcg attatcgggc tttactccac gcagtcgcgg
     8101 cttagtccgc gacgtgcgga ttcacacagt tcttctcaac ctttccttgt aacgcatcaa
     8161 tcaaattatc cacggcacag gcggccatgc cataacgcgt ctcatgggtg gcagatccaa
     8221 tatgcggtac tgcgacgacg ttggccattg agagcaacgg cgaatctacg gaaagtggct
     8281 cttgttcgaa gacatccagc ccggcggcgt gaatttcccc tttctgcaat gctgcgatca
     8341 gtgcattttc gtcaaccacc gggccacgtc cggcattaat gaaaatggcg gaggatttca
     8401 ttttggcgaa ttgttctgcg ccaaacagat gatgcgtctc atcagttaac ggcaggatca
     8461 ggcaaacgaa atctgactct tgtaacagtg tatccaaatc gcagtagcgg gcgttgaagc
     8521 gttcttctgc ttctttatgg tggcggcgcg cgttatagag gatgggcatg ttgaagccaa
     8581 agtgcgcacg ttgtgccagc gccataccga tccgtcccat cccgacaatg cccagtgttt
     8641 tatggtgaac gtcagtgccg taccagtccg ggcctatgct cgcggtccat tcgcctgctt
     8701 ttacccgttc tgctacctcc acaacccgac gagcggtaga caacaccagc gccatcagcg
     8761 tatcggcgac ggtttctgtt aagacggttg gcgtgtgcat cagcagaatt tttcgggcgg
     8821 taagcgcatc gacatcaaaa ttgtcatagc cgacggagat cgttgatgtg gcacgcagtt
     8881 tcggcatttt ttccagcaat gcggcatcaa cattctcgtt tgaacccagt aaaccttcag
     8941 cttcggcaaa aattgctgca ttttgttcga cggtttgtgg gctgaggttt gccacctggt
     9001 gaacggtgaa atgctcttgc aggcgttgca gtaaatcatc aggtaaggct ttgtagagga
     9061 taacggacgg cttcatgctt ctctccattc actgataagg cgatcacctt agcctgaaat
     9121 atgatgaagg aaaactgaag aatgccaggt gatttctgca tcacctggca tgaaagggat
     9181 tacagcgggc ttaaggtaat ttctacacgg cggttttgcg ccttaccttc tgcggtgctg
     9241 ttgctggcga ttgggttagc cgggccaagg ccctgagtac ggatgcggct ggcgtccacg
     9301 ccctgggtga tcaacgcgct ggcaacggaa tccgcacgct gctgggagag acgcatgttc
     9361 agatcgtgac cacccgtgct gtcagtataa ccaatcacgt taaccgccgt tttcggatac
     9421 tctttcagca ccattgccac gccggtcagg gtgttagcgc ccgccggttt cagggttgcg
     9481 ctgctgctgt cgaaggtcac attgttcggc atattgagga taatgttatc cccgctgcgg
     9541 gttacgttaa caccagtgcc gcgcattttg tcacgcagct tcgcttcctg cacatccatg
     9601 taataaccca cgccgccgcc cagagctgcg cctgctgctg cgccaatcag cgcgccttta
     9661 ccgcgatctt tcttcgaaga agagagcgca ccaatacccg cgcccacgag agagcccaga
     9721 cctgcgccga tagcagattt acctgcttcg cgttcgccgg tgtaagggtt agttgtgcag
     9781 ccagataccg ccagagcgcc actcactacg gcggcaataa gataaacacg tttcttcatt
     9841 gttaatcctt aataaccttt ttattctttg ccacgggttc cgtggcggga gattatgccg
     9901 cgtgaacatg aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct
     9961 caatacatct aacctttcag gagtcttcgg tttgaccaac tcatcctcac gatattccgt
    10021 tctgactgcc gcccattggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag
    10081 ctcgcgtggc gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgtgacca
    10141 ggttcacagc aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc
    10201 ggaaaacccg caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc
    10261 gctggatctt attcaccaac aacataaacg cattcgtgag gcttatggtc cggcatcgat
    10321 ttttgctggt tcctacggct ggcgttcaaa cggcgtgctg cataaggcct cgacattatt
    10381 acaacgctat atggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg
    10441 cgcggcacag gcgatcatgc cgtatgtcgt gggtggtagt gaagtttatc aacagcagac
    10501 cagttggccg ctggtgctgg aacatagcga tgtcgtggtg ctgtggagtg ctaacccact
    10561 caatacgctg aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttctgc
    10621 actgcgtgac agcgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga
    10681 tttctttggc gataaaatgg agtgggtggc accgcacatg ggcactgatg ttgcgctgat
    10741 gctggggatc gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg
    10801 ttgcaccacc ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc
    10861 gaaaaccgcc gaatgggcag cagagatttg tggtgttaac gcagagaaaa tccgcgagct
    10921 ggcggctatt ttccaccaaa ataccaccat gctgatggct ggctggggaa tgcagcgcca
    10981 acagtttggt gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat
    11041 cggcacaccc ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtaaccccac
    11101 gcggcgcgct gcggtactct cttccatgca gggcagcttg ccgggtggct gcgatgcggt
    11161 agataaaatc cctgtcgccc gcattgttga agcactggaa aaccccggtg gcgcatatca
    11221 acacaacggt atggaccgac atttcccgga tattcgtttt atctggtggg cgggcggtgc
    11281 caactttact catcatcagg ataccaatcg cctgatccgt gcctggcaaa aaccggagct
    11341 ggtggtgatc tctgaatgct tctggacggc ggcggcaaaa cacgcggata tcgttctgcc
    11401 tgcgactacc tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca
    11461 tctggtgccg atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt
    11521 ttttgccgag ttaagtgaac gctgggagaa gggcggttat gcacgtttta cggaaggaaa
    11581 aagtgagctg caatggctgg aaacgtttta taacgttgcc cggcagcgcg gggcaagcca
    11641 gcaggttgaa ttgccgccat ttactgagtt ctggcaagcc aaccagttaa ttgagatgcc
    11701 ggaaaacccg gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgcaggc
    11761 gcatccatta aaaaccgcca gcggtaagat tgaaatcttc tcgcagcgta ttgccgatta
    11821 cgcttatccg gattgcccgg ggcatccaat gtggctggtg ccggacgaat ggcagggcaa
    11881 tgccgaaccg gaacagttac aggtactttc tgctcatccg gcgcatcgtc tgcacagcca
    11941 gctgaattac agttctctgc gcgaattgta cgcggtggca aaccgtgagc ctgtcaccat
    12001 tcatcctgac gatgcccagg cgcgcggcat acaagatggc gatactgttc ggctgtggaa
    12061 cgcacgcggg caaattcttg ccggagcggt cattagcgag ggaattaaac ctggcgtgat
    12121 ttgcattcac gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa
    12181 cggcgcagtg aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc
    12241 gggtaatacg gcgctggcat ggctagaaaa atacaacggt ccggaactga cacttacagc
    12301 gtttgaacca ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg
    12361 caatcgacaa tgtgaaaacc ctgcgcctgg taaaaatcta tcgccggttg atttttttga
    12421 taaacctcca gcatcagatg gggatagcgc tgctgcacat actgcatcag cgccttacca
    12481 ataccgcgtc tgacggcctt cggtgcgaca aacattgctg ccagaaatcg gccttccata
    12541 atgctgacaa aaccgagaag cttaccgtct tcttcccaga cccagttttt cgcattggca
    12601 agatacgcat cccgcaccag cggaatgcac tcgcgccagt aattcacttt tataaaggga
    12661 tgcccccagg ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc
    12721 gcttcccgaa tcatggttta tttcccggat agcaacagca gccaaccaca tgatcattca
    12781 ccagcccaca tgcctgcata aaggagtaac agattgtggt accgacaaac ttaaaaccac
    12841 gttttttcag tgccttagat agggcgtcgg aggcgggcgt agatgtgggg atttcgctca
    12901 acgttgtggc ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcggcaa
    12961 acggttcgcc gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa
    13021 ttttcccgcg atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct
    13081 gcattgcggc aaccttcacc ggatcgaact gatgaaagca ggcgcgatag ttttcgcgtt
    13141 ttttgaggac ggtgatccac gataacccag cctgctgccc ttccaggcag atcatttcga
    13201 acagtttttt cctgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa
    13261 gcgggtcctg actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg
    13321 taaaaatttc agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc
    13381 tactatgccc gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg
    13441 gagtcgtgtt aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga
    13501 gtgtggtaat cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg
    13561 ttgatacgca acccgggcat tccacagagc gttacacctg ggatagtgat catcaacctg
    13621 attacaacga tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag
    13681 tcaatctggc ggaagaaacc cctgtggatg tgaccagcag tatgagtatg ggctggaatt
    13741 ttcctttata tgaacaggtt acaactggcc cggtcgcggc attacattac gatggcacaa
    13801 ccacctcgat gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc
    13861 atgccagcgt gagtacctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct
    13921 gggcgcaaat cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc
    13981 tgagccggat gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg
    14041 atatgttgct caatcaaaat attgccgcct atgccgcgct atctcaggca gaaaatacca
    14101 ctaacaatag cgattatctg tatacgatgg gggttagtgc cagattttaa cgtaacaatc
    14161 acaattgaaa ccattgaata acaatagttg tggcgatagt gggtgctaac ttaccaaata
    14221 ataaatttgg tgaataattg tcgcgtcatt cattcctgaa ctaaggcatt tcattccgtt
    14281 ctgatggcat ttcatgccgt ttttccccag gcataaagtg cacttcgtta tggttgtcgg
    14341 cagagatttt tcctttttat tactgcagga atactgccat gacaccttca aattatcagc
    14401 gtacccgctg gttgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt
    14461 atacctggag cctgtttaac ggcgcgcttt ccgccaagct ggatgcgccg gtaagccagg
    14521 tcgctttctc tttcggcttg ttaagtctgg ggctggcaat atcgtcttct gttgcgggca
    14581 aattgcagga acgttttggt gttaaacgcg tcaccatcgc ttccggcatt ttgctgggat
    14641 tagggttctt cctgacagcg cattctgaca acctgatgat gctgtggtta agcgccggtg
    14701 tgctggtcgg tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaaat
    14761 ggttcccgga gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta
    14821 gcctgggttt caaatttatc gacacgcagc tgctggaaac ggtcggtctg gaaaaaacct
    14881 ttgtgatttg gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag
    14941 acgcaccaaa acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg
    15001 cagagtcgat gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca
    15061 tgagcggcct gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg
    15121 atgtggtttc tgcagccaat gcagtcactg ttatttccat cgccaacctt tcaggtcgtc
    15181 tggtgctggg cattctgtct gacaaaatcg cccgtatccg tgttatcacc attggtcagg
    15241 taatttcgct ggtgggtatg gcggccctgc tgtttgcacc attgaatgca gtgacgttct
    15301 ttgcagcgat tgcctgcgtg gcatttaact ttggcggcac tattaccgtc tttccgtcac
    15361 tggtcagtga gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg
    15421 gtttcggtat cggtagcatt tgtggttcga ttatcgcctc actgtttggc ggcttctatg
    15481 tgaagttcta cgtcatcttt gctctgctga ttctgtcatt ggcgctttct acgacgattc
    15541 gtcagccaga gcagaaagta ttgcgtgagg cgcatattta gccagaaact acaggttttg
    15601 aacgaaaggg ggaggaaatc ctccttttta ttgcatttta aagaataaaa atgaaaatat
    15661 tttatcttat tatttgcgtg gtgaatgttt ttgttttgtt taattgatat tttatattta
    15721 tt
//
LOCUS       DAZFBR010000108        15499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFBR010000108 DAZFBR010000000
VERSION     DAZFBR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.108"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(137..418)
                     /locus_tag="ACHJZ2_003793"
     CDS             complement(137..418)
                     /locus_tag="ACHJZ2_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HFO4569826.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYRASSTAKGVTTEVVAVGNNDKRKIVLNRYD"
     gene            complement(680..1789)
                     /gene="apbC"
                     /locus_tag="ACHJZ2_003794"
     CDS             complement(680..1789)
                     /gene="apbC"
                     /locus_tag="ACHJZ2_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO4569827.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1921..3954
                     /gene="metG"
                     /locus_tag="ACHJZ2_003795"
     CDS             1921..3954
                     /gene="metG"
                     /locus_tag="ACHJZ2_003795"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO4569828.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4095..4823
                     /locus_tag="ACHJZ2_003796"
     CDS             4095..4823
                     /locus_tag="ACHJZ2_003796"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFO4569829.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQIKSFAD
                     VAAAEKAKLKLIAEKTKKGYVENASANVHIPPITKATPEVETSPESKNQRPWLADDAV
                     IPVTDDINRFAFPHRSRPREINYLRKDGEIWKRIADNTRAYDPDNNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLVIRCGLESAVEIVLFALQL
                     KYKPVKGAVTTIIPPNHEAESLPS"
     gene            6780..7904
                     /locus_tag="ACHJZ2_003797"
                     /pseudo
     CDS             6780..7904
                     /locus_tag="ACHJZ2_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7917..10901
                     /locus_tag="ACHJZ2_003798"
                     /pseudo
     CDS             7917..10901
                     /locus_tag="ACHJZ2_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            10911..14540
                     /gene="yehI"
                     /locus_tag="ACHJZ2_003799"
     CDS             10911..14540
                     /gene="yehI"
                     /locus_tag="ACHJZ2_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO4569830.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            14602..14919
                     /gene="yehK"
                     /locus_tag="ACHJZ2_003800"
     CDS             14602..14919
                     /gene="yehK"
                     /locus_tag="ACHJZ2_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO4569831.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         4155 a         3848 c         3737 g         3759 t
ORIGIN      
        1 ctataacaaa catttttgaa actgattaat taccgtaaat taccgtaaat aacatttatg
       61 ggatgaataa aaaagccaaa atcattcata ggaatgattt tggcttttta aatatgctta
      121 aaacggtgtt ggcaaattaa tcgtagcgat ttaacacgat tttgcgtttg tcgttattac
      181 ccacagctac cacttctgtc gtaacgcctt ttgcagtaga agaagcacga tagttggttt
      241 caactcgttg taacaactgc ccacaacgtt gctgaagatg attattaaca acatccatac
      301 tgcatgtagg ggaactaatt ctgccattga tggttaatgt tgtagtatca gaagagaacg
      361 cgggtgatgc tagtccataa gcaagaaaaa cagctcctga caaccaatac ttattcattg
      421 aacgttatcc ctgtagtaaa ggttatgccg ggtagattga gtgcataaca cactatagct
      481 gtacatccgc tacacagcca cgagggatga taaagagaca gtggttgcac aatcaatcgc
      541 ctttgtacag tttcggacta agatccactg aatattcgca gtattaaata agtgttcatt
      601 aatgcggcaa aaacaaatac accgcatcaa tataacatct ctgcggtgta tgaagaataa
      661 ccagaatggt taataggcgt tagaccgcgc ggaaggaaat ctcgcctgga atgacttcac
      721 cctgccagta gagctgagct gcaacgcggt cagccagctg gcggtagata gcggtaaatt
      781 cgctctccgg acggctgata accgttggcg tgcctttatc gagatcctca cgtaaggaga
      841 tgtgtaacgg catctgcccc agcagctgtg tatggtattt ttcagccagt ttctctgcac
      901 caccggtacc gaaaatcggt tcatggtgac cacagttact gcaaatatgc acactcatgt
      961 tctcgacaat accgagcacc ggaacctcga ctttctcgaa catcacaatg ccttttttcg
     1021 catcgatcaa cgcgatgtcc tgcggggtgg tgaccacgac tgcaccagtt actggaatgt
     1081 tttgcgccag cgtcaattga atgtcaccgg tgcctggcgg catatcaagc accagatagt
     1141 cgagatccgg ccacaaggtt tcctgcaaca tctgcattaa cgccttgctc gccatcggtc
     1201 cacgccacac catcgcattg tcgtcagtga ccagataacc aattgaattg gttgccaggc
     1261 catgagacat aatcggtgcc atatgggtac cgtccggcga agttggacgc tgattttctg
     1321 cgcccagcat ggtgggaatt gatgggccgt agatatcggc gtccagaatg ccgactttcg
     1381 ccccttccgc cgccagtgcc agcgccaggt ttaccgccgt ggaggattta cccacgccgc
     1441 ctttaccgga actgaccgca atgatgttct tcacgccatt aatacccggc tgattcttca
     1501 cgcgttttaa ggtggcgata ttgtgcgaca gtttccagtc gatagctttc gccccggtga
     1561 tacgcagcag ttcggcgcta cattgctctt ttaactcttc aaacgcgcta tgccagacga
     1621 atggcataac cagttccaca tgcaacgtgt cgtccatcca ggcgacatgg tgcaacgctt
     1681 tcagcgtggt gaggttatgc ttcagggttg ggtgctgaaa attggccagt gtcccggcga
     1741 ccattgcgcg caaggcttct ggtgatttgg cctgggattg ttcgttcatc ccgactcctt
     1801 ttttgtatag ataaaccatc agctgatagt ttacctgaag aatatagaga agtacttact
     1861 taacattttc ccatttggta ctatctaacc ccttttcact attaagaagt aatgcctact
     1921 atgactcaag tcgcgaagaa aattctggtg acgtgcgcac tgccgtacgc taacggctca
     1981 atccacctcg gccatatgct ggagcacatc caggctgatg tctgggtccg ttaccagcga
     2041 atgcgcggcc acgaggtcaa cttcatctgc gccgacgatg cccacggtac accgatcatg
     2101 ctgaaagctc agcagcttgg tatcaccccg gagcagatga ttggcgaaat gagtcaggag
     2161 catcagactg atttcgcagg ctttaacatc agctatgaca actatcactc gacgcacagc
     2221 gaagagaacc gccagttgtc tgagcttatc tatactcgct tgaaagaaaa cggttttatt
     2281 aaaaaccgca ccatctctca gctgtacgat ccggaaaaag gcatgttcct gccggaccgt
     2341 tttgtgaaag gcacctgccc gaaatgtaaa tccccggatc aatacggcga taactgcgaa
     2401 gtctgcggcg cgacctacag cccgactgaa ctgatcgagc cgaaatcggt ggtttctggc
     2461 gctaccccgg taatgcgtga ttctgaacac ttcttctttg atctgccctc tttcagcgaa
     2521 atgttgcagg catggacccg cagcggtgcg ttgcaggagc aggtggcaaa taaaatgcag
     2581 gagtggtttg aatccggtct gcaacagtgg gatatctccc gcgacgcgcc gtacttcggt
     2641 tttgaaattc cgaacgcgcc gggtaaatat ttctacgtct ggctggatgc gccgattggc
     2701 tatatgggtt ctttcaagaa tctgtgcgac aagcgcggcg acagcgtaag cttcgatgaa
     2761 tactggaaga aagactccac cgccgagctg taccacttca tcggtaaaga tattgtttac
     2821 ttccacagtc tgttctggcc agcaatgctg gaaggcagca acttccgcaa gccgaccaac
     2881 ctgtttgttc atggctatgt gacggtgaac ggcgcgaaga tgtctaagtc tcgcggtacc
     2941 tttattaaag ccagcacctg gctgaatcat tttgacgccg acagcctgcg ttactactac
     3001 actgcgaaac tctcttcgcg cattgatgat atcgatctca acctggaaga tttcgttcag
     3061 cgcgtgaatg ccgatatcgt taacaaagtg gttaacctgg cctctcgtaa tgcgggcttt
     3121 atcaacaagc gttttgacgg cgtgctggca agcgaactgg ctgacccgca gttgtacaaa
     3181 accttcactg atgccgctga agtgattggt gaagcgtggg aaagccgcga atttggtaaa
     3241 gcagtgcgcg aaatcatggc gctggctgat ctggctaacc gctatgtcga tgaacaggct
     3301 ccgtgggtgg tggcaaaaca ggaaggccgc gatgctgacc tgcaggcgat ttgctcaatg
     3361 ggcatcaacc tgttccgcgt gctgatgact tacctgaagc cggtactgcc gaaactgacc
     3421 gagcgcgcag aagcatttct caatacagaa ctgacctggg atggtatcca gcaaccgctg
     3481 ctgggccaca aagtgaatcc gttcaaggcg ctgtataacc gcatcgatat gaagcaggtt
     3541 gaagcactgg tggaagcgtc taaagaagaa gtgaaagcag ctgccgcgcc agttactggc
     3601 ccactggcag atgatccgat tcaggaaacc atcacctttg acgacttcgc caaagttgac
     3661 ctgcgcgtgg cgctgattga aaacgcagag tttgttgaag gttctgacaa actgctacgc
     3721 ctgacgctgg atctcggcgg tgaaaaacgc aatgtcttct ccggcattcg ttctgcttac
     3781 ccagatccgc aggcactgat tggtcgtcac accattatgg tggctaacct ggcaccacgt
     3841 aaaatgcgct tcggtatctc tgaaggcatg gtgatggctg ccggtcctgg cgggaaagat
     3901 attttcctgc taagcccgga tgccggtgct aaaccgggtc atcaggtgaa ataatccccc
     3961 ttcaaggcgc tgcatcgaca gcgccttttc tttataaatt cctaaagttg ttttcttacg
     4021 attttgtctc tctctaaccc gtataaatac tggtagcatc tgcattcaac tggataaaat
     4081 tacagggatg cagaatgaga cactttatct atcaggacga aaaatcacat aaattctggg
     4141 cggttgagca gcagggaaac gagctgcata tcaactgggg caaagtcggc actaacgggc
     4201 aaagccagat aaaaagtttt gcggatgttg cagcagcgga aaaagcgaaa ctgaagctta
     4261 tcgcggagaa aacgaaaaaa ggctatgtgg aaaatgcttc agcaaacgtg catatccccc
     4321 ccatcaccaa agccactcct gaagttgaaa cttcccctga gagtaaaaac caacgcccgt
     4381 ggctggctga tgatgcagtt attccggtaa ctgacgatat taaccgattt gcttttcctc
     4441 atcgctctcg ccccagagag atcaattatc tgcgcaaaga cggtgagata tggaagcgta
     4501 ttgcagataa caccagggca tatgatcccg acaacaatta ccgctcttat cctgaaaact
     4561 ggcagcaggc ttttgctgag ttacaaaggc gaattctgga taatcaacag acagggagtg
     4621 cgcaatctga tgcagcattg ctctggagtt tttggaactc ttactctgca gatgaactgg
     4681 tcgatgactt agtcattcgc tgtggtctgg aaagcgccgt tgaaatcgtc ctttttgccc
     4741 ttcaactcaa atataaacca gtaaaaggcg cagtaaccac catcattcca cctaatcacg
     4801 aagcagaatc gctacctagt tagcatcaac gtctatgtca tcatctttca ctcgcttcag
     4861 aagatgagtg gcaacgctgt gtagataaag tactcaccgc cattccgacg ctatcccccg
     4921 cacggcaacc ttttgcggcg ttactgctcc ctgaacgccc ggatatcgcc catgctatag
     4981 atctacgcta tgcagaccaa aacgtcccgg cgatgacctg gttaagactg gtgataaccg
     5041 atgatactgc tctgagtaca gtggaaaaat acgacttccc accgttgtat cgtgattttc
     5101 gtaattttcg cgcttatctg gcaatgttgt tggcaaataa tggggtgcgc ggtgtaagcc
     5161 ggatacttct tgagtttacg gaagaccatt ctgacaatcc cacttatctc ttcgaacgca
     5221 tcagcgagac tgaaaatctt gtcaaatggc tatggaaaac gaatcacccg gatgcgattc
     5281 aaattctgat cctcggtgta attggtaaga aaaagcacct ggaatactta agcaaggcca
     5341 gtcaaaagca tcccgctgcg gctattgcgg cttatgctac tttgctggca atacatgaag
     5401 ataaagagtg gcgtaaagcg cttgtcaaac tgattaccgc cacgccagag ttagtatgcg
     5461 acgtcattcc ctgggttaat gctaaagcgg caggcatctt atctgaatgt cgtccgcagt
     5521 cagtcgctga agaatgtgaa tatgccactg tggatatgtt gccggagctg cttgtttcgc
     5581 cgccctggat gaccaaagag aaaaagaaaa atacgcctgt gtttgatctt ccagtgcttc
     5641 cagttccttc tgtttctgat gtcactccag agattaccaa aaaactgacc cgtacttacc
     5701 ttgtcaccca cttccagcaa atagcccaac agcaggcgac aaaacaaaca ttgtttaccg
     5761 acctgccgcc gatcaaaaaa gcaagctggg agaaacactt aataccgctt acaccggagc
     5821 aacaaatact gtggcatctt ggttttgaaa aatggcggga gagcggagaa aaaatatacg
     5881 aaaaaattcc ggcgccacaa agcgccgttg atgccctgct acgcttcgat tttccagcac
     5941 tcaatgctga atttgtgcat tatcataaca atgcatacaa aagctggaat ctcattgccc
     6001 tctgttacct gcctggtcaa caggccattt cttttcttaa ccagatagtc aaagaagata
     6061 actactctgg tgagggaaat attctggcaa tttttggtag cgccgccata cctgcattta
     6121 tggcctgcct gcaacgtgat ccacgacgtc tgtgcttctt cccctttttc ctcggtgtca
     6181 gtgaactggc cttaccgatg gcgcaacaat tacagaaaaa aatgtcttat gaagatgcgc
     6241 gaaactggct aactgattat ccacgccatg ccgccgccgg gctgcttcct gtggccctgg
     6301 gcaaaaaagg aaaagatcgt gattgtgccc gccaggcgct tcgtctgctg gtgaatttaa
     6361 accagcgaga aacgattgag gaaatcgcgc aaggatataa tcaacctgat gttttagctg
     6421 cgctggcaac attgttcgat agcgacccac tggaagaata tcccgctaaa atcgccccac
     6481 tgcccggctt ttatcagttc accttgtggc gcagaccacg gcttaaaagt aacaacctgc
     6541 ctctgtcaga tgacgctatg cgccacctcg gcactatgct gagctttcct cgcgacatta
     6601 ccgcctacgc tgggctggat atcattagag aaatcttcac ccgcgaatca ctggctgaat
     6661 ttggctggga tctgtatccc gcctggacag aagctggcgc acccgcaaaa gaaaactggg
     6721 catttacttc gcttgggatt ttaggcaatg acgacaccgc gcgtaaatta accccgctta
     6781 tccgcgcctg gcctggggaa tcccagcata aacgggcggt gtccgggctg gatgtattag
     6841 ctgatattgg cagcgatgtt gcgctaatgc tgcttaatgg catcgcgcga aaaattaaat
     6901 tcaaagcatt acaggaacat gcccgcgaga aaatcaacat agttgctgaa aaccgtgggc
     6961 tgactatggc tgaactggaa gaccgcctgg ctccagattt agggcttgat agcagtggct
     7021 cgctgatact ggatttcggc ccccgcaaat tcaccgttgg ttttgacgaa accttaaaac
     7081 ctgtagtgtg cgatgcaaac ggcaaagtcc tgaaagattt acctaagcca aaccagagcg
     7141 atgaaaaaac tcaggcaact gacgcggtta atctcttcaa acagttgaaa aaagatgtac
     7201 gcgccatagc cagccagcag attgatcgtc tggaacaggc tatgtgccag cgcagacgct
     7261 ggacggcaga gcagttccgc ctgtttctgg tggagcatcc gctggtacgt cacttaaccc
     7321 gacggctgtt atggggggtt tataacgatg aaaacgccct tatcacctgt tttcgcgtgg
     7381 cagaagacag cacttacagc gatgcgcagg atgagttatt cacgctgcca gcaggaaaca
     7441 tcggtattcc gcatgtgctg gaaatatccc ctgaatccgc cgctgcattc aggcaaattt
     7501 acgctgatta cgaactgctt ccccctttcc aacagctcga gcgaggtagc tatcaccttg
     7561 ctgataatga acgtaatact cacgaactga cacgctggca aggacggctt tgccaggccg
     7621 gacgcattgt cgggctggaa cgcagaggct ggcaacgtct ggaagaaagc ggcagcgttt
     7681 atgcgatgcg caaaacaacc cctcatggcg atctcgaact tgaaacggaa cctttttcat
     7741 taatttatgg tgaaacgggg tatggcgacc agcacccggt tgaaagcgta aaaatcacct
     7801 cgccagatga tcgctacggt aaacaatcct cactcacttt ctccatgctc gacgacatca
     7861 ccgccagcga gctgattaac gacattgaat cactgtttga ttaaaaggat ttaacaatga
     7921 aaacctatat ttatcaggac gaaaaatcac ataaattctg ggcggtggag cagcagggaa
     7981 acgagctgca tattaactgg ggtaaagtcg gtaccaacgg gcaaagtcag gtaaaaagct
     8041 ttgcagatac tgcggcggcg gaaaaagcag cactgaagct gattgcggag aaggtgaaga
     8101 aggggtatgt ggagcaagta gacgctaacg ctcactcaca tgatgcctca gcaaagaaag
     8161 tcctggtcac agaggaaaaa gcctcaataa atcgccaggc agcaactaat ggtttaccct
     8221 ggctggcaga caacgatccc attattcttc ccccgaatat tgcccgccat gccctcagcc
     8281 accgcctttg gccgggagag ccagtaaaaa aaccaaccaa acccgagaaa caaaatttaa
     8341 tttcccgatt ggcaaatcgt acttatcatt attataaaaa tctgactttc gattacagct
     8401 catgctctga tgaatgggct gatgcgatta gccaggcaat cagcttaatt aaggcccaaa
     8461 agactgagat acccgcgcgt actatggcgg tacttacttt tctggaaata tgtttttgtc
     8521 agggctggaa tgatgaaacc tcatccgagt tgctggatga aattgtaagc accttcggta
     8581 tcgaatacgc aactgaaact gtcatcttct ggtggcaaat tgatttcgat tttgactata
     8641 aagatgaaca tttgactttc tttattaatt atgcagatag cagcaaggac agttatagaa
     8701 ggaataacga caaatttagt ttacgtctgc gtaaacattt atcactagca gaagaagaag
     8761 tatggcaaaa ttgcattgca aaattactcg ttgcgttacc agatatatca ctctatcgcc
     8821 aaccgttaat tgccatttta atgccagaaa tacccgaagt ggcccatgag atcgtacatc
     8881 gtttacacca ggtcgcagat gttccacaac ttgaaatgtt gaaactggta gcgacagatc
     8941 cattcacgct cgaaatactg gagaactatc aatacattga tgtatttaac acctatggtg
     9001 ccagttggtc agctacggta ttacgggagc aaggtatagc ggcactggct cgcctgactc
     9061 cctatgctga agaagataat tgcggtgacg tactgaaatg tattaatcat ccgttggcca
     9121 ttacgaataa aaagtgccga aaattttcct tctgcaacga tagtggcatt agccgaagca
     9181 ttggtaaaaa aagatgataa acgcaaacat acattgctgg tttcattact taatgcaaat
     9241 cctttatttg tcgaaaaatt agtcatcgga ttatcgtcgc aagcggcaaa tttattgcaa
     9301 caatgtcagc aattgctgaa tagtcgcaat gaagattttg caaactcaga aattctacca
     9361 atggtactag tctcgccgcc ctgggaaact caaaataaaa ataacgacaa cagtcctgtg
     9421 ggtaaatacc cggcgaaaat ccccccttta ccgttgtttt atcaacctcc attatggact
     9481 cgcccgaaac tcaaaaatag cagcaaggta ctgccagata gcgcactcca gcacctcggt
     9541 gaaatgctcc gcttccctca ggaagaggcc ctgtatccgg gattattgca ggtgaaagat
     9601 gcctgtaccg ccgactcact ggctgaattt gcctgggatc tgtttaccgc ctggctgact
     9661 gcgggcgcac cgtcgaaaga gagttgggcg ttcactgcgt taggcgttct tggtaacgat
     9721 gacaccgccc gcaaactgac gccattaata cgcgcctggc ctggtgaatc ccagcataaa
     9781 cgcgccaccg ttgggttgga tattctcgcc gctatcggta gtgatatcgc cctgatgcag
     9841 cttaacggta ttgcccagaa actgaaattc aaagcattac aggagcgggc aaaagaaaaa
     9901 attgccggca ttgccgaaag ccgcgaactc acggtggcag agcttgaaga tcggttagca
     9961 ccggatctcg gtctggatga taacggttcg ttgctgctgg atttcggctc acgccagttc
    10021 accgtcagct ttgatgaaac cttaaaaccg tttgtgcgtg atgtttctgg cagccgtctg
    10081 aaagacctgc ccaaaccaaa caaaagcgat gatgaatcac aggcgaacga tgctgttaac
    10141 cgctacaaac tgctgaaaaa gatgcgcgta ccgtcgccgc ccagcaggta gcaaggttgg
    10201 aatccgccat gtgcctgcgt cgccgctggt cgccagaaaa ctttcagctc ttcctggttg
    10261 aacatccact ggtacgccac ttaacccgcc gtctgatttg gggcgtttat agcaccgaaa
    10321 accagctact gacgtgcttc cgcgtggcgg aagataacag ctacagcacc gctgacgatg
    10381 atcttttcac cctgccggaa ggcgatatct ctgttggcat tcctcacgtt ctggaaatat
    10441 caccgacgga tgctaccgcc tttggtcagc tttttgccga ctatgaactg ctaccaccgt
    10501 tccgtcagct tgaccgtaac agttacgccc tgacagaagc cgagcgcaac gccagcgaac
    10561 tgacccgctg ggcaggcaga aaatgcccga gcggtcgggt catggggctg gcgaataaag
    10621 gctggataaa gggcaccccg caggatggag gctggatcgg ctggatgatc aaacctctgg
    10681 gccgctggtc gttaatcatg gaaatcgatg aaggttttgc ggtaggcatg tcgccagccg
    10741 aactgagtgc cgagcaaatc ttaagcaagc tgtggctatg ggaaggcaac gcagaaagct
    10801 atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat gccatcaccg
    10861 ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc atggacaagg
    10921 aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa ggcaaaacgc
    10981 cgctcagtca tcgtcgctgg ccgggcgaac cagtgtccgt tatcactgga agtctcatcc
    11041 agacattggg agatgaatta ctacaacaag caggccagaa ggaaaatatc acctggaatt
    11101 atgataaatg ctcactggag tggcagtccg ccatccagca ggcaatcaat ttgactggcg
    11161 agcataaacc gtcaatccca gcccttacca tggcggctct gatctgtatc gcgcaaaatg
    11221 acagccagca gttacttgat gaaatcgtgc aacaagaggg gctggaatat gcgacggatg
    11281 tagtgatatc gcgtcagtgc attgcgcggc gttatgagag tgactcactg gtcgtcactt
    11341 tgcagtatca ggacgaggac tatggctacg gttatggctc cgcgacctat aacgatttcg
    11401 atctccgttt gcgtaagcat ctgtcgctgg cagaggaaag ttgctggcag cgttgtgccg
    11461 ataaactcat tgccgcactg ccaggaatac ccaaaattcg ccgtcctttt attgcgctga
    11521 tcctcccgga aaaacctgaa atagccaatg aactcgccag tcttgaaagc tcccggtcaa
    11581 gtctccattc aaaggagtgg ttaaaggtcg ttgctactga taatacggcg gtaaaaaaac
    11641 tcgaacgata ctggggcctg gacgtattta gcgatcgcga agccagctac atgtcacaag
    11701 aaaaccgctt cggctacgcg gcctgcgcct cccttttgcg cgaacaagga ctggcagccg
    11761 ttccgcgcct ggcaatgtat gctcataaag aagattgcgg cagtctgctg gtacaaatta
    11821 accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa cccagcctgc
    11881 aacgtgtggc taaatacagt aaaaacttcc cccatgcgac gctcgccgca ctggcagaac
    11941 tgctggcgtt aaaagaacca ccagcccgcc ctggttatcc aatcatcgaa gacaaaaagc
    12001 tgcctgcaca gcaaaaagct cgagaagaat actggcgtac gctgttacaa acgctgatgg
    12061 catcgcagcc acaactggca gcagaagtga tgtcgtggtt aagtactcaa gccagggcag
    12121 tgctgaagag ttatttatcg gcactgccaa aaccggttat tgatggcacc gataacagca
    12181 atctgcctga aatcctcgtt tcaccaccgt ggcgtagtaa gaaaaaaatg acagctccac
    12241 gtcttgattt ggcacggctc gaattaactc cgcaagttta ctggcaacca ggcgaacgag
    12301 agaggcttgc cgccactgag tctgcccgtt atttcagcac ggaatctctt gcgcaacgca
    12361 tggaacaaaa aagtgggcga gttgtattac aggaactggg ttttggggat gatgtatggc
    12421 tgtttttgaa ttatatactc cctggaaaac tggatgctgc acgcaattca ctcattgttc
    12481 agtggcatta ctaccagggg cgggttgaag agatcctgaa tggctggaac tccccggaag
    12541 cacaattagc agaacaggcg ctccgcagcg gtcacataga agcgttaatt aacatatggg
    12601 aaaatgacaa ctactcacgt tatcgtccgg aaaagagtgt ctggaacctg tatttattgg
    12661 cacagttgcc gcgtgagatg gctttgactt tctggctgcg tatcaatgag aaaaagcatc
    12721 tgttcgcggg agaggactat tttctcagta tcctcggatt ggatgcgcta ccaggtctgc
    12781 tgttggcttt ttcacatcgt ccaaaagaaa catttccgtt aattttaaat ttcggcgcaa
    12841 cagaactggc cctgcccgtt gcccgcgtct ggcgacgttt cgcggcgcag cgtgatctgg
    12901 ctcgccagtg gattttacaa tggccggaac atacggctac tgcacttatc cctcttgtct
    12961 tcaccaaatc tagcgataaa agcgaagctg cattacttgc cctgcgttta ctttacgaac
    13021 atgggcatgg cgaattgcta caaactgtag ccaatcgttg gcagcgtaca gatgtatggc
    13081 ctgccctgga gcatttactg aaacagggtc ccatggaaat ttacccggca cgcattccaa
    13141 aagcccctga tttctggcat ccgcaaatgt ggtccaggcc gcgccttatc actaataatc
    13201 aacctgttac cgatgacgct ctggaaatta tcggcgaaat gctgcgcttt acccaggggg
    13261 gacgttttta tagcgggctg gaacaactga aaacgttctg ccagccacaa acgctggcag
    13321 cttttgcctg ggatctcttc actgcgtggc aacaagctgg tgcccccgca aaagacaact
    13381 gggcatttct ggcgttaagt ctctttggtg acgaaagcac ggcacgggat ctaacaacac
    13441 agatcctcgc ctggccacaa ggcaaatctg cccgtgctgt cagcggcctg aacatcctta
    13501 ccctgatgaa taatgatatg gcgctgatac agctgcatca tatatcgcaa cgggctaaat
    13561 cccgcccctt acgtgataac gcggcggaat ttcttcaggt agtcgcagaa aatcgcgggc
    13621 taagccagga agagctagcg gacagattag tcccaaccct gggccttgat gatccgcagg
    13681 cgttgagttt tgattttggt ccccggcagt ttaccgttcg cttcgatgaa aacctcaacc
    13741 cggttatctt tgatcagcaa aacgttcgcc agaaaagcgt tccccggttg cgcgccgatg
    13801 acgatcaact gaaagcgccc gaggcactgg cccgactaaa agggctaaaa aaagatgcta
    13861 ctcaggtgag caaaaacctg ctcccgcgtc ttgaagctgc cctacgtacc acccgacgct
    13921 ggtcgctggc agattttcat tctctgtttg ttaatcatcc ctttacccgt ctggttaccc
    13981 agcgattaat atggggggtt tatccggcaa atgaaccgcg tcgtttactc aacgcctttc
    14041 gtgtggccgc agaggggggg ttctgcaatg cgcaagatga gccaattgac ctgcctgcgg
    14101 acgctctgat tggcattgcc cacccgttag aaatgacagc agaaatgcgc agtgaatttg
    14161 cacagctttt tgccgattac gaaattatgc cgccttttcg ccagttggcg cgccgcacgg
    14221 tgctgctcac acctgatgag tcaaccagta acagcctgac tcgctgggaa ggtaaatccg
    14281 ctaccgttgg gcaacttatg ggaatgcgat acaaaggctg ggagtcaggt tatgaggacg
    14341 catttgtcta tgacctgggt gagtaccggc tggtccttaa gttttcaccc ggttttaacc
    14401 actacaatgt tgatagcaaa gcgttaatga gcttccgttc tcttcgagtg taccgtgaca
    14461 ataaatccgt cacttttgcc gaacttgatg tgtttaattt gagtgaggcg ttaagcgcac
    14521 ctgacgtcat tttccattaa aaacacagcg ggcagtgatg caactgcccg ttatcaacac
    14581 gacttaccag cggggaaagc gatgatcgtg caaaaagagc tggttgctat ttacgattat
    14641 gaggtccctg tacctgaaga tccgttttcc ttcagacttg agatccataa atgctctgaa
    14701 ttatttacag gttccgtcta tcgactggag cgattccggc tacgttcaac atttcatcaa
    14761 cgtgatcgag aagatgctga cccgctaata aatgatgcgt tgatttatat aagagatgag
    14821 tgtattgatg agcggaaatt acgaggtgaa tcacctgaaa ctgtaatagc aatttttaat
    14881 cgtgaactac agaatatatt caaccaagaa atagaataat atactctaga taattaaagt
    14941 cgcagcacat gcaacttgaa gtatgacgag tatagctttc ttaaaggggg aaacggtatt
    15001 cccccttaga tgcgtttcaa tttgcccgtt acttcaacaa atcaatcaac cgtaacagag
    15061 ccactaataa ctccagtacg gcaatgagca cggttaactt ttgtgtcagc gtgtccatcc
    15121 gctctcctta ttaaggagcg caacaccagc accttaccct tacactgcct gtcgatactg
    15181 gtttacgtat gttggtacga aacgttgctc tccggatcag ggcaccgcct gcggcaccac
    15241 ccgtccggca gccaggactt aaaattggtc cgatccggca caacctccca acgggcagga
    15301 ttataaatct cactcactga attgcaatgg agtgtggata tggttataat ttattcgttg
    15361 gtacgaaatg ttgcacaatc agcaagttag cgtcactgta gtaaataaaa gaacctgcct
    15421 caccagcagg tttttttatt ttctgcgatc tactttccag atattttttt gccaatagcc
    15481 caccataaat attcacatc
//
LOCUS       DAZFBR010000109        15086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFBR010000109 DAZFBR010000000
VERSION     DAZFBR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.109"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            1..971
                     /gene="ybeQ"
                     /locus_tag="ACHJZ2_003801"
                     /pseudo
     CDS             1..971
                     /gene="ybeQ"
                     /locus_tag="ACHJZ2_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(1041..1523)
                     /gene="ybeL"
                     /locus_tag="ACHJZ2_003802"
     CDS             complement(1041..1523)
                     /gene="ybeL"
                     /locus_tag="ACHJZ2_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO4569832.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1759..4341
                     /gene="leuS"
                     /locus_tag="ACHJZ2_003803"
     CDS             1759..4341
                     /gene="leuS"
                     /locus_tag="ACHJZ2_003803"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO4569833.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4356..4937
                     /gene="lptE"
                     /locus_tag="ACHJZ2_003804"
     CDS             4356..4937
                     /gene="lptE"
                     /locus_tag="ACHJZ2_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO4569834.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4937..5968
                     /gene="holA"
                     /locus_tag="ACHJZ2_003805"
     CDS             4937..5968
                     /gene="holA"
                     /locus_tag="ACHJZ2_003805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO4569835.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5970..6611
                     /gene="nadD"
                     /locus_tag="ACHJZ2_003806"
     CDS             5970..6611
                     /gene="nadD"
                     /locus_tag="ACHJZ2_003806"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO4569836.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            6635..7246
                     /gene="cobC"
                     /locus_tag="ACHJZ2_003807"
     CDS             6635..7246
                     /gene="cobC"
                     /locus_tag="ACHJZ2_003807"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO4569837.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7506..7823
                     /gene="rsfS"
                     /locus_tag="ACHJZ2_003808"
     CDS             7506..7823
                     /gene="rsfS"
                     /locus_tag="ACHJZ2_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO4569838.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7827..8294
                     /gene="rlmH"
                     /locus_tag="ACHJZ2_003809"
     CDS             7827..8294
                     /gene="rlmH"
                     /locus_tag="ACHJZ2_003809"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO4569839.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8325..10226
                     /gene="mrdA"
                     /locus_tag="ACHJZ2_003810"
     CDS             8325..10226
                     /gene="mrdA"
                     /locus_tag="ACHJZ2_003810"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO4569840.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10229..11341
                     /gene="mrdB"
                     /locus_tag="ACHJZ2_003811"
                     /gene_synonym="rodA"
     CDS             10229..11341
                     /gene="mrdB"
                     /locus_tag="ACHJZ2_003811"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO4569841.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11352..12440
                     /gene="rlpA"
                     /locus_tag="ACHJZ2_003812"
     CDS             11352..12440
                     /gene="rlpA"
                     /locus_tag="ACHJZ2_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO4569842.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12580..13791
                     /gene="dacA"
                     /locus_tag="ACHJZ2_003813"
     CDS             12580..13791
                     /gene="dacA"
                     /locus_tag="ACHJZ2_003813"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO4569843.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13901..14164
                     /gene="ybeD"
                     /locus_tag="ACHJZ2_003814"
     CDS             13901..14164
                     /gene="ybeD"
                     /locus_tag="ACHJZ2_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO4569844.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14265..14906
                     /gene="lipB"
                     /locus_tag="ACHJZ2_003815"
     CDS             14265..14906
                     /gene="lipB"
                     /locus_tag="ACHJZ2_003815"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO4569845.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3716 a         3826 c         3939 g         3605 t
ORIGIN      
        1 tcacgtcaag ttgctgcgat aatttatcaa tagatgagat tatcgaacgt gctgaaaaag
       61 gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc gcaattgact
      121 ctccggacga cgaaaaggcc ttttactagc tgaagctggc cgctgagcaa ggtcattgtg
      181 aagcacagta ttccttaggg caaaagtata ccgaggataa aagccgtcat aaagataatg
      241 agcaagccat cttctggctg aaaaaagctg ccctacaagg ccatactttc gcttccaacg
      301 cccttggctg gatactggat cgtggagaag accccaacta taaagaagcg gttgcctggt
      361 atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttggg tggatgtaca
      421 gaaatggcaa cggagccgca caagactata cgctggcatt tttctggtac aaacaagctg
      481 cattacaagg ccatagtgac gcgcaaaaca atctggccga tctttatgaa gacggaaaag
      541 gcgttgctca aaacgagaca ctcgccgcat tctggtattt gaaaagcgca cagcagggta
      601 atcggcatgc tcagtttcaa attgcatggg attataacgc tggcgaaggg gtggaccagg
      661 actataagca agcgatgtac tggtatctga aggctgccgc tcaggggagc gtcggcgctt
      721 acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat tatcaggctg
      781 cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg tataacctgg
      841 ccattatgta tcactacgga gaaggaagac ctgtcgatct ccgacaggct ctcgacctgt
      901 atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt gagactgaag
      961 atttactgta gagataaaaa caagggccgg ggaatttcat tcggcccata ttctctttat
     1021 tgctgtgcga aatcagacta ttacggctca aacgggcgtc tctggaactg gtcatgacca
     1081 catttcgggc atagcgtcag cacttccggt gtgtagatcg ggagatggaa gtgacatttc
     1141 tcgcagacca gatttcccag cccgaccact tctccgctgt gataaacccc atgatgattg
     1201 aggtcctgga aaacttcgcg ccattcaagc tgcgttttat cggtgatgtc tgccagctcc
     1261 tgccacaagc tttctttaat cacccgcata aagacgctgt cagattcttc tttcaggctc
     1321 tcttcatagc tcatggcgaa ctcttccagg tcacgtctga cagctcgcgt cagctcatcg
     1381 acctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg ttccaccagt
     1441 gcgtcgatat cacgttcgcc attgcgcagg cgttcgctca gtgacgcaac cagttcacgg
     1501 taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga ctataataat
     1561 agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat taatgtatga
     1621 ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg cggacgtttg
     1681 ggctatgcta tgcggatctg aaaaaccacc tctaacgcta cttttgtagc cgtaatgaaa
     1741 acaggaccac tggctgccat gcaagagcaa taccgcccgg aagagataga atccaaagta
     1801 cagcttcatt gggatgagaa gcgcacattt gaagtaaccg aagacgagag caaagagaag
     1861 tattactgcc tgtctatgct tccctatcct tctggtcgac tacacatggg ccacgtacgt
     1921 aactacacca tcggtgacgt gatcgcccgt taccagcgca tgctgggcaa aaacgttctg
     1981 cagccgatcg gctgggacgc gtttggtctg cctgcggaag gcgcggcggt gaaaaacaac
     2041 accgctccgg caccgtggac gtacgacaac atcgcgtata tgaaaaacca gctcaaaatg
     2101 ctgggctttg gttatgactg gagccgcgag ctggcaacct gtacgccgga atactaccgt
     2161 tgggaacaga aattcttcac cgagctgtat aaaaaaggcc tggtatataa gaagacttct
     2221 gcggtcaact ggtgcccgaa cgaccagacc gtactggcga acgaacaagt tatcgacggc
     2281 tgctgctggc gctgcgatac caaagttgaa cgtaaagaga tcccgcagtg gtttatcaaa
     2341 atcactgctt acgctgacga gctgctcaac gatttggata aactggatca ctggccagac
     2401 accgttaaaa ccatgcagcg taactggatc ggtcgttccg aaggcgtgga aatcaccttc
     2461 aacgttaacg actatgacaa cacgctgacc gtttacacta cccgcccgga cacctttatg
     2521 ggttgtacct acctggcggt agctgcgggt catccgctgg cgcagaaagc ggcggaaaat
     2581 aatcctgaac tggcggcctt tattgacgaa tgccgtaaca ccaaagttgc tgaagctgaa
     2641 atggcgacga tggagaaaaa aggcgtcaat actggcttta aagctgttca cccattaacg
     2701 ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga tggagtacgg cacgggcgca
     2761 gtaatggcgg ttccggggca cgaccaacgc gactacgagt ttgcctctaa atacggcctg
     2821 aacatcaaac cggttatcct ggcggctgac ggctctgagc cagatctttc tcagcaagcc
     2881 ctgactgaaa aaggcgtgct gttcaactct ggcgagttca acggtcttga ccatgaagcg
     2941 gccttcaacg ccatcgccga taaactgact gcgatgggcg ttggcgagcg taaagtgaac
     3001 taccgcctgc gtgactgggg ggtttctcgt cagcgttact ggggcgcgcc gattccgatg
     3061 gtgacgctgg aagacggtac cgtaatgccg accccggacg accagctgcc ggtgatcctg
     3121 ccggaagatg tggtgatgga cggcattacc agcccgatta aagcagatcc acagtgggcg
     3181 aaaactaccg ttaacggtat gccagcgctg cgtgaaaccg acactttcga cacctttatg
     3241 gagtcctcct ggtactatgc gcgctacact tgcccagagt acaaagaagg tatgctggat
     3301 tccgaagcgg ctaactactg gctgccggtg gatatctaca ttggtggtat cgaacacgcc
     3361 attatgcacc tgctctactt ccgcttcttc cacaaactga tgcgtgatgc aggcatggtg
     3421 aactctgacg aaccagcgaa acagttgctg tgtcagggta tggtgctggc agatgccttc
     3481 tactatgttg gcgaaaacgg cgaacgtaac tgggtttccc cggttgatgc tatcgttgaa
     3541 cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg caggccatga actggtttat
     3601 accggcatga gcaaaatgtc caagtcgaag aacaacggta tcgacccgca ggtgatggtt
     3661 gaacgttacg gcgcggacac cgttcgtctg tttatgatgt ttgcttctcc ggctgatatg
     3721 actctcgaat ggcaggaatc cggcgtggaa ggggctaacc gcttcctgaa acgtgtctgg
     3781 aaactggtgt acgagcatac tgcgaaaggt gatgttgcgg cactgaacgt tgatgcactg
     3841 actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa ctatcgctaa agtgaccgat
     3901 gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg cgattatgga gctgatgaac
     3961 aaactggcga aagcaccgac cgatggcgag caggatcgcg ctctgatgca ggaagcactg
     4021 ctggccgttg tccgtatgct taacccgttc accccgcaca tctgcttcac gctgtggcag
     4081 gaactgaaag gcgaaggcga tatcgacaac gcgccgtggc cggttgctga cgaaaaagcg
     4141 atggtggaag actccacgct ggtcgtggtg caggttaacg gtaaagtccg tgctaaaatc
     4201 accgttccgg tggacgcaac ggaagaacag gttcgcgaac gtgctggcca ggaacatctg
     4261 gtagcaaaat atcttgatgg cgttactgta cgtaaagtga tttacgtacc aggtaaactc
     4321 ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg atatctggca acattgttgt
     4381 tatctctggc ggtgttaatc accgccgggt gtggctggca tctgcgtgac accacgcagg
     4441 ttccttccac tatgaaggtc atgatcctgg actcaggcga tccgaacggg ccattaagcc
     4501 gtgcggtgcg taaccagtta cgtctgaatg gtgtcgagtt gcttgataaa gaaaccacgc
     4561 gtaaggacgt tccatccctg cgtttgggtg ctgtatcaat ctcacaggat accgcgtcgg
     4621 ttttccgtaa tggtcaaact gcggaatatc agatggtgat gactgttagc gcgtcggtat
     4681 tgattcctgg acgagatatc tacccgatta gcaccaaagt cttccgttca ttcttcgata
     4741 acccgcaaat ggcgttagcg aaagataacg agcaggaaat gatcattaaa gagatgtacg
     4801 accgtgctgc cgaacagctg attcgtaagc tgccaagcat ccgtgctgcg gatattcgtt
     4861 ccgacgaaga acagacgtcg actacaacgg atactccggc aacgcctgca cgcgtctcca
     4921 ccacgctggg taactgatga ttcggttgta cccggaacaa ctccgcgcgc agctcaatga
     4981 agggctgcgc gcggcgtatc ttttacttgg taacgatcct ctgttattgc aggaaagcca
     5041 ggacgctgtt cgtcaggtag ctgcggcaca aggattcgaa gaacaccaca ctttttccat
     5101 tgatcccaac actgactgga atgcgatctt ttcgttatgc caggccatga gtctgtttgc
     5161 cagtcgacaa acgctattgc tgttgttacc agaaaacgga ccgaacgcgg cgatcaatga
     5221 acaacttctc acactcaccg gacttctgca tgacgacctg ctgttgatcg tccgcggtaa
     5281 taaattaagc aaagcgcaag aaaatgccgc ctggtttact gctcttgcga atcgcagcgt
     5341 gcaggtgacc tgtcagacac cggagcaggc tcagcttccc cgctgggttg ctgcgcgcgc
     5401 aaaacagctc aacttagaac tggatgacgc ggcgaatcag gtgctctgct actgttatga
     5461 aggtaacctg ctggcgctgg ctcaggcact ggagcgttta tcgctgctct ggccagacgg
     5521 caaactgaca ttaccgcgcg ttgaacaggc ggtgaatgat gccgcgcatt tcactccttt
     5581 tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc gcattgcata ttcttcagca
     5641 actgcgtctg gaaggcagcg aaccggttat tttgttgcgc acattacaac gtgaactctt
     5701 gttactggtt aacctgaaac gccagtctgc ccatacgcca ctgcgtgcgt tgtttgataa
     5761 gcatcgggta tggcagaacc gccggggcat gatgggcgag gcgttaaatc gcttaagtca
     5821 gccgcagtta cgtcaggccg tgcaactcct gacacgaacg gaactcaccc tcaaacaaga
     5881 ttacagtcag tcagtgtggg cagagttgga agggttatct cttctgttgt gccataaacc
     5941 cctggcggac gtatttatcg acggttgata tgaaatcttt acaggctctg tttggcggca
     6001 cctttgatcc ggtgcactat ggtcatctaa aacccgtgga aacgctggcg aatttgattg
     6061 gtctgacgcg agtcacaatc atccctaata atgttcctcc gcatcgtccc cagccggaag
     6121 cgaacagcgt acagcgtaaa cacatgcttg aactggcgat tgccgacaag ccattattta
     6181 ctcttgatga acgcgagcta aagcgcaatg ccccctctta cactgcgcaa acactgaaag
     6241 agtggcggca ggaacaaggg ccggacgtgc cgctggcgtt tattattggt caggattcat
     6301 tgctgacctt tccgacctgg tacgaatacg aaacgatact cgacaatgca catttgatcg
     6361 tctgtcggcg tccaggttac ccacttgaaa tggcgcaacc gcaataccag caatggctgg
     6421 aagatcattt gacacataac ccggaagatc ttcaccttca gcctgccggt aaaatttatc
     6481 tggctgaaac gccgtggttt aacatctcgg cgaccatcat ccgcgaacgt ttgcaaaacg
     6541 gtgaatcatg tgatgattta ttgccggaac cggtactgac ttacattaac caacaaggct
     6601 tgtatcgctg atacctgtcg ttacggaggg catgatgcga ctgtggttaa ttcgtcatgg
     6661 cgaaacacaa gcgaatgtcg atggtctgta cagcggtcat gcgcccacac ccctgaccgc
     6721 acgcggtatt gagcaagcgc aaaatctgca tacactgcta gatgatgttt cctttgacct
     6781 ggttttatgc agtgaactgg aacgggcaca gcataccgcg cgactggttc tcagtgaccg
     6841 ccagctcccc gttcatatca tccctgaact caacgaaatg ttttttggcg actgggagat
     6901 gcgacatcat cgcgacctca tgcaagaaga tgccgaaaac tatagcgcgt ggtgcaatga
     6961 ctggcagcat gctatcccca cgaacggtga aggatttcag gcattttcgc aacgtgtgga
     7021 acgctttatc gcaaggctta gtgaatatca gcactatcag aatattttaa tcgtcagcca
     7081 tcagggcgta cttagtctgt taattgctcg tttaattggc atgcctgccg agtctatgtg
     7141 gcattttcgc gttgaccagg ggtgttggag cgccattgat ataaaccaaa aattcgcgac
     7201 gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaat gcatgacttt tctgtttttt
     7261 tacgggtaag ccgcaacgac cattgacagc cccgggcagg ctgatattct ccgcagccag
     7321 actttttccg ccagacacga ctttgtagaa attgttttac aaaaatggcg atgcaatctg
     7381 cggcgcgggg tgggatgata gcccactttc gaaagccgat tcggcgacaa ttgtcccgaa
     7441 atcgcctctg gttcaggtat actgacagac catttttatc tatttgattc acccaggggg
     7501 aaaacttgca gggtaaagca ctccaggatt ttgttatcga caaaattgat gacctcaaag
     7561 gtcaggacat catcgcctta gacgttcagg gcaaatccag catcactgac tgcatgatca
     7621 tctgtacggg tacgtccagc cgtcatgtta tgtccattgc tgaccacgtt gtgcaggagt
     7681 ctcgcgcagc gggcctgtta ccgctcggag tagaaggtga aaacagcgcc gactggattg
     7741 tcgtggattt gggcgatgtg attgtccatg tcatgcagga agagagccgt cgcctgtatg
     7801 aactggaaaa actctggagt taatgcgtga agctgcaact tgtcgccgtg ggaacgaaaa
     7861 tgccggactg ggtacaaacc ggttttaccg agtacctgcg tcgttttccg aaagatatgc
     7921 ccttcgagct gattgaaatt ccggccggaa aacgcggcaa gaatgcggac atcaagcgca
     7981 tactcgacaa agagggtgag cagatgttgg cggccgcagg caaaaaccgc attgtcaccc
     8041 tcgatattcc aggcaagccc tgggatacgc cgcagttagc cgctgagctg gaacgctgga
     8101 agctggatgg tcgcgacgtc agtctactga ttggcgggcc tgaagggttg tcgcctgcct
     8161 gtaaagcggc ggcggagcag agctggtcgc tgtcagcgct taccctcccc catccgctgg
     8221 ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag catcaccacc aaccatcctt
     8281 atcaccgtga gtgataaggg agctttgagt agaaaacgca gcggatgaaa ctacagaact
     8341 cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg ccgggcgctg gtcgcctttt
     8401 tggggatttt gctgctgacc ggcgtgctta tcgccaacct gtataatctg caaattgttc
     8461 gctttaccga ctaccagacc cgctctaatg aaaaccgcat taagctggtg cctatcgcgc
     8521 ccagccgcgg cattatctac gaccgtaacg gtatccctct ggccctcaac cgcactatct
     8581 accagataga aatgatgccg gagaaagtcg ataacgtgca gcaaacgctg gacgctttgc
     8641 gcagcgtggt tgatctgacc gatgacgata ttgctgcatt ccgaaaagag cgcgcacgtt
     8701 cacaccgttt cacctccatt ccggtgaaaa ctaacctgac cgaagtacaa gtagctcgct
     8761 ttgccgtcaa tcagtaccgt tttccgggtg ttgaagttaa aggctataaa cgtcgttact
     8821 atccttacgg ttcggcgttg acccacgtca tcggctatgt gtcgaaaatc aacgataaag
     8881 acgtcgaacg cctgaataat gacggcaaac tggccaacta tgcggcaacg catgatatcg
     8941 gtaagctggg cattgagcgt tactatgaag acgtgctgca cggtcagacc ggttatgaag
     9001 aggttgaagt taacaaccgt gggcgtgtta tccgccagtt aaaagaagtc ccaccgcaag
     9061 ccggacacga tatttacctg acgctggatc tcaaactcca gcaatatatt gaaacgctgc
     9121 tggcgggtag ccgcgcagct gtggtagtca ccgatccgcg tacaggtggg gtgctggcgc
     9181 tggtttccac gcctagttat gacccaaact tgtttgttga cggtatctcc agcaaagatt
     9241 attccgcctt gttgaacgac ccgaatacac cgctggtgaa ccgcgccaca cagggggttt
     9301 atcctcccgc gtctacagtt aaaccctatg tggcggtttc ggcattgagc gccggggtga
     9361 tcacgcgcaa tacgacgctg tttgaccctg gctggtggca actgccaggt tcggaaaaac
     9421 gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa tgtcacaaga tcgctggaag
     9481 aatctgcgga taccttcttc tatcaggtgg cctacgatat ggggatcgat cgcctctccg
     9541 aatggatggg taaattcggt tatggtcatt ataccggtat cgacctggcg gaagaacgtt
     9601 ccggcaatat gcctacccgc gaatggaaac agaaacgctt taaaaaaccg tggtatcagg
     9661 gtgacaccat tccggttggt atcggtcagg gttactggac agcgacccca atccagatga
     9721 gtaaggcact gatgatcctg attaatgacg gtatcgtgaa ggttccgcat ttgctgatga
     9781 gcaccgccga agacggcaaa caggtgccat gggtacaacc gcatgaaccg cccgttggcg
     9841 atattcattc cggttactgg gagctggcga aagacggtat gtacggtgtt gctaaccgcc
     9901 ctaacggtac ggcgcataaa tactttgcca gcgcaccgta caaaattgcg gcgaaatctg
     9961 gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta taatgcgcac aaaattgccg
    10021 agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc atacaacaat ccgcaagtgg
    10081 ctgtcgccat gattctggag aacggtggcg cgggtccggc ggttggtacg ctgatgcgcc
    10141 agatcctcga ccacattatg ctgggtgata acaacaccga tctgcctgcg gaaaatcctg
    10201 cggttgccgc agcggaggac cattaatcat gacggataat ccgaataaaa aaacattctg
    10261 ggataaagtc catctcgatc ccacaatgct gctgatttta ctggcattgc tggtttacag
    10321 cgccctggtt atctggagcg ccagcggtca ggatattggc atgatggagc gtaaaatcgg
    10381 ccaaatcgcg atgggtctgg tcatcatggt ggtgatggcg caaattcctc cacgcgttta
    10441 tgaaggctgg gccccctatc tctatatcat ctgtattatt ttgctggtgg cggtagatgc
    10501 tttcggtgcc atctctaaag gcgctcaacg ctggctggac ctcggtattg ttcgttttca
    10561 gccgtcggaa attgccaaaa tagccgtacc actgatggtt gcgcgcttta tcaaccgcga
    10621 cgtttgcccg ccatcgttga agaacactgg catcgcgctg gtgctgatat ttatgcccac
    10681 gctgctggtg gctgcacagc ctgacctggg aacatcaatc ctcgttgcgc tttccggtct
    10741 gtttgtactg ttcctctctg gccttagctg gcgtctgatt ggcgtcgcag tagtgctggt
    10801 agcggcgttc attccgattc tgtggttctt cctgatgcat gattaccagc gccagcgcgt
    10861 aatgatgctc ctggacccgg aatcagaccc actcggcgcg ggctatcaca ttattcagtc
    10921 taaaattgct attggctccg gcggattacg cggcaaaggc tggctgcacg gcactcagtc
    10981 acagcttgaa tttctccccg aacgccatac tgactttatc ttcgcggtac tggcggaaga
    11041 gctgggatta gtgggcattt tgattctgct cgctctctac attctgctga tcatgcgcgg
    11101 gctgtggata gccgccagag cgcaaaccac ctttggtcgc gtcatggctg gcggcttaat
    11161 gctgatatta ttcgtttatg tcttcgtaaa tattggtatg gtaagcggta ttctgccggt
    11221 tgtaggggtt ccgctcccac tggtcagtta tggaggatcg gcgctaattg tgctgatggc
    11281 tgggttcggg attgtaatgt caatccacac ccacaggaaa atgttgtcga aaagcgtgta
    11341 agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat cgcggcagga atgctcgcgg
    11401 catgtacaag cgatgatggt cagcaacaga cagtaagtgt accgcagcct gcggtatgta
    11461 acggccccat agttgaaatt agcggggcgg acccgcgttt cgaaccactg aacgcgacgg
    11521 caaatcagga ttaccagcgc gacggtaaaa gctacaaaat cgtgcaggat ccgtctcgat
    11581 ttatccaggc gggactggcg gcaatctatg atgccgaacc aggcagtaac ctgacggcct
    11641 ctggcgaagc tttcgatccg acacagctga cggcggccca tccaacgctt ccgatcccca
    11701 gctacgccag aatcactaac ctggctaacg ggcgaatgat cgtggtgcgc attaatgatc
    11761 gcggtcctta cggcaacgac cgcgttattt cgctttctcg cgcagcagct gaccgtctta
    11821 acacgtcaaa caacaccaaa gttcgtatcg atccgattat tgtcgcccag gatggttcgc
    11881 tttctggtcc tggtatggct tgtaccacag tcgccaaaca gacttacgcc ctgcctgcac
    11941 ctcccgattt aagcggtggc gcgggaacaa gttcagtgtc tggcccgcag ggtgacattc
    12001 ttccggtcag taattcgacg ctaaaaagcg aagatccgac cggcgcgccg gtaaccagca
    12061 gcggtttcct cggcgcacca acgaccttag cgccaggtgt actggaaggc agcgaaccga
    12121 cgcctgctcc acagcccgtt gttacagctc cgtcgacaac gcctgcaacc tcgcctgcaa
    12181 tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa ctttatggtg caggtcgggg
    12241 ccgtaagcga tcaggctcgt gcgcaacagt accaacagca actgggacag aagttcggcg
    12301 tccccggtcg cgtaactcaa aatggcgcgg tctggcggat ccagcttggc ccattcgcca
    12361 acaaagccga agccagtacc ttgcagcaac gtttgcaaac cgaagcccaa ttacagtcat
    12421 ttattaccac cgcgcagtag cgtaaagcag gcatctgatg tgtcaatttc tgtaaatgac
    12481 gttaacaaag tcacgcggaa agtcagatgc ctgccggtag tgcatttgct atagtagggc
    12541 acttttttta attccatcac ggatgtcgta gttcagacca tgaataccat tttttccgct
    12601 cgtatcatga agcgcctggc gctcaccacg gctctttgca cagcctttat ctctgctgca
    12661 catgccgatg acctgaatat caaaactatg atcccgggtg taccgcagat cgatgcggag
    12721 tcctacatcc tgattgacta taactccggc aaagtgctcg ccgaacagaa cgcagatgtc
    12781 cgccgcgatc ctgccagcct gaccaaaatg atgaccagtt acgttatcgg ccaggcaatg
    12841 aaagccggta aatttaaaga aactgattta gtcactattg gcaacgacgc atgggccacc
    12901 ggtaacccgg tgtttaaagg ttcttcgctg atgttcctca aaccgggcat gcaggttccg
    12961 gtttctcagc tgatccgcgg tataaacctg caatcgggta acgatgcttg tgtcgccatg
    13021 gccgattttg ccgctggtag ccaggacgct tttgttggct tgatgaacag ctacgttaac
    13081 gccctgggcc tgaaaaatac ccacttccag acggtacatg gtctggatgc tgatggtcag
    13141 tacagctccg cgcgcgatat ggcgctgatc ggccaggcgt tgatccgtga cgtaccgaat
    13201 gaatactcga tctataaaga aaaagaattt acgtttaacg gtattcgcca gctgaaccgt
    13261 aacggcctgt tatgggataa cagcctgaat gtcgacggca tcaaaaccgg acacactgac
    13321 aaagcaggtt acaaccttgt tgcttctgcg actgaaggcc agatgcgctt gatttctgcg
    13381 gtgatgggcg gacgtacttt taaaggccgt gaagccgaaa gtaaaaaact gctaacctgg
    13441 ggcttccgtt tcttcgaaac cgttaaccca ctgaaagtag gtaaagagtt cgcctctgaa
    13501 ccggtttggt ttggtgattc tgatcgcgct tcgttagggg ttgataaaga cgtgtacctg
    13561 accattccgc gtggtcgcat gaaagatctg aaagccagct atgtgctgaa cagcagtgaa
    13621 ttgcatgcgc cgctgcaaaa gaatcaggtc gtcggaacta tcaacttcca gcttgatggc
    13681 aaaacgatcg aacaacgccc gctggttgtg ttgcaagaaa tcccggaagg taacttcttc
    13741 ggcaaaatca ttgattacat taaattaatg ttccatcact ggtttggtta aaaattgaac
    13801 acttgaaagt gtaatttccg tccccatata ctaagcatca gtaaaaaact cccgccttct
    13861 ggcgggagtt gctatttaat tacgttacgc cggagctgac atgaaaacca aacttaacga
    13921 actgcttgaa ttccctactc cttttactta caaagttatg gggcaggcgt tacctgagct
    13981 ggttgatcag gtggttgaag tggtacagcg ccatgcgcca ggtgactaca ccccaacggt
    14041 aaaaccaagc agcaaaggca actaccactc ggtatctatc actatcaacg ccactcatat
    14101 cgagcaggtt gaaacactgt atgaagaact gggcaaaatc gatattgtcc gcatggttct
    14161 gtaactcgct tctccgttac ccggcccctg gtcgggtaac tcccctctcc tcgctgtgat
    14221 atacttttct cccactttta ctcattctcc acggagatgc cgttttgtat caggataaaa
    14281 ttcttgtccg ccagctcggt cttcagcctt acgagccaat ctcccaggct atgcatgaat
    14341 tcaccgatac ccgcgatgat agtacccttg atgaaatctg gctggtcgag cactatccgg
    14401 tattcaccca agggcaggca ggaaaggcgg agcacatttt aatgcccggt gatattccgg
    14461 tgattcagag cgatcgcggt gggcaggtga cttatcacgg gccggggcaa caggtgatgt
    14521 atgtattgct taacctgaaa cgccgtaaac tcggtgtgcg tgaactggtg accttgcttg
    14581 agcaaacagt ggtgaatacc ctggctgaac tgggtataga agcgcatcct cgggctgacg
    14641 cgccaggtgt ctatgttggg gaaaagaaaa tttgctcact gggtttacgt attcgacgcg
    14701 gttgttcatt ccacggtctg gcattaaacg tcaatatgga tctttcacca tttttacgta
    14761 ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat atcacaatgg aaacccgaag
    14821 cgacgactaa taatattgca ccacgtttac tggaaaatat tttagcgcta ctaaacaatc
    14881 ctgacttcga atatattacc gcttaatttc atacctcaat ggcccaattt acactgggtc
    14941 attactcata acacctgcta atgctttaca tctccttcgc gtttgtatat tttctttatc
    15001 acttgcagaa atgcgatgct acgccttgct ccgtaactca ccccattgat taacttattg
    15061 aaatagttga ttatgatgag caaaag
//
LOCUS       DAZFBR010000110        15033 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFBR010000110 DAZFBR010000000
VERSION     DAZFBR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.110"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(75..2354)
                     /gene="maeB"
                     /locus_tag="ACHJZ2_003816"
     CDS             complement(75..2354)
                     /gene="maeB"
                     /locus_tag="ACHJZ2_003816"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO4569846.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2643..3593
                     /gene="tal"
                     /locus_tag="ACHJZ2_003817"
     CDS             2643..3593
                     /gene="tal"
                     /locus_tag="ACHJZ2_003817"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4569847.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILSLTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3613..5616
                     /gene="tkt"
                     /locus_tag="ACHJZ2_003818"
     CDS             3613..5616
                     /gene="tkt"
                     /locus_tag="ACHJZ2_003818"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4569848.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5711..6754)
                     /gene="ypfG"
                     /locus_tag="ACHJZ2_003819"
     CDS             complement(5711..6754)
                     /gene="ypfG"
                     /locus_tag="ACHJZ2_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO4569849.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6880..7455)
                     /gene="nudK"
                     /locus_tag="ACHJZ2_003820"
     CDS             complement(6880..7455)
                     /gene="nudK"
                     /locus_tag="ACHJZ2_003820"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO4569850.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            complement(7523..9502)
                     /gene="aegA"
                     /locus_tag="ACHJZ2_003821"
     CDS             complement(7523..9502)
                     /gene="aegA"
                     /locus_tag="ACHJZ2_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO4569851.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLIRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9708..11408
                     /gene="narQ"
                     /locus_tag="ACHJZ2_003822"
     CDS             9708..11408
                     /gene="narQ"
                     /locus_tag="ACHJZ2_003822"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO4569852.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11572..14685
                     /gene="acrD"
                     /locus_tag="ACHJZ2_003823"
     CDS             11572..14685
                     /gene="acrD"
                     /locus_tag="ACHJZ2_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO4569853.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14784..14843)
                     /gene="ypfM"
                     /locus_tag="ACHJZ2_003824"
     CDS             complement(14784..14843)
                     /gene="ypfM"
                     /locus_tag="ACHJZ2_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO4569854.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3521 a         3986 c         3923 g         3603 t
ORIGIN      
        1 taagctttac taatatattg cagctaaacc gcttaccctg aatattcagg gtaagcgtga
       61 gagttaaaaa agaattacag cggttgggtt tgcgcttcaa ccacggccag cgccaccatg
      121 ttgacgatac gacgcaccga tgcgatcggc gttaacacgt gaaccggttt cgccacgccc
      181 atcagcaccg gaccgacagt cacaccttcc gagctggaaa cgcgcagtaa gttgtaacta
      241 atgcgggcag cttccatgtt cggcatcacc agaatattgg cagaaccttt caaagggctg
      301 tccggcatac ggtcgttgcg aatcgcttcc accagcgctg catcgccgtg catttcacca
      361 tcaatcatca gttctggcgc acgttccctg accagttcca gcgcctgacg cattttgctc
      421 gacgacgggc agtcagaaga accaaagttg gagtgcgaca acagagcaac gcgcggttca
      481 ataccgaaac gacggacagt ttctgccgcc atcaaggtga tctccgccag ctcttctgcg
      541 tctggttcgt cattgacgta ggtatcggca ataaaggtgt taccactcgg cagcagcagc
      601 gcgttcattg cacctgcggt gtgaacgcca tcgcgataac caaagacatt tttcaccacg
      661 ctaaaatgtt catgataatc acccaccgta ccgcaaatca ttgcatcggc ttcgccacgc
      721 tgaaccatga tcgcgccgat cactgtcggg ttactgatca gcgcccgctg cgcctgttcc
      781 tgagtgacgc cgcgacgctt catgatctgg aagtattcgg tccagtactc tttaaagcgc
      841 ggatcggatt cgttattgac gatctcaaaa tcaacgcccg ctttgatctg caagcccagt
      901 ttctgaatgc gcatttcgat cacgttcgga cgaccgataa ggatcggttt cgccagtccc
      961 agcgttacca gttcctgagt ggcatgcaga acgcgcgcct cttccccttc cggcagaact
     1021 acgcgcttcg gcgctttgcg agcctgggag aaaatcggct tcataaacag gttggttttg
     1081 taaacgaact cagtcagctt gtcgatgtag acgtcgaaat cagcaatcgg acgcgtcgcc
     1141 acgcccgact ccatcgccgc tttcgcgacc gcaggagcaa tcttgactat cagacgcgga
     1201 tcaaacggtt ttggaatgat gtattcaggg ccaaagctca gatcctgatc gccatacgct
     1261 gaagccacca cttcactctg ttccgcatgg gccagttctg caatcgcgcg taccgccgcc
     1321 agtttcatct cttcgttgat ggcggttgcg ccaacgtcca gcgcgccacg gaagatgaac
     1381 gggaagcaaa ggacgttgtt cacctggttc gggtagtcgg aacggccggt acagatgatg
     1441 gcatccggac gcacttcttt cgccagcggc ggcagaattt ccggttccgg gttcgccagc
     1501 gccaggatca ttggcgctcg agccattttc ttcaccattt cctgggtcag cactttcggg
     1561 ccggaacagc cgaggaaaat atctgcgcct tcaatcacat catcgagggt acgtttgccg
     1621 tcatccacta ccgcatatgc ggctttggtt tccgccatgt ttggctcacg gccctgatag
     1681 ataacgcctt ttgaatcgca aaccacgatg ttatgttttt gcagacccaa cgctaccagc
     1741 aggttcatac aggcgattgc tgcagcaccc gcgccggaaa ccaccatacg cacgtcagag
     1801 atgtttttct ccaccacgcg caagccgttg aggatggcgg cagtgctgat aattgccgtg
     1861 ccgtgctgat cgtcgtggaa taccggaata ttcatccgct cgcgcagttt ctgttcaata
     1921 tagaaacatt ctggcgcttt aatgtcttcg aggttgatgc cgccgaaggt cggttcgagc
     1981 gcagcgacga cttcaataaa tttgtccggg tcgagttcat caacttcaat gtcaaataca
     2041 tcaatcccag cgaatttctt aaacagaacg cccttgcctt ccatcaccgg tttgcctgcc
     2101 agcgcgccaa tgttgcctaa ccccagcacc gccgtaccgt tagagatcac cgccaccagg
     2161 ttaccgcgcg cggtatattt gtaggctttt aacgggtctt tttcgatttc aagacaaggt
     2221 gcggcaacgc ctggtgagta ggccagcgcc agatcgcgct gcgttgccag aggcttggtt
     2281 ggagaaacct ggattttccc tggaactgga aattcatgga aatcaagtgc actttgtttt
     2341 aactggtcat ccatttggtt gttcctttca cgtaacgttc acaaataaag tgtgtgggca
     2401 acagcccctg cccacaacgt ggcgcacatt attaccttgc cggagtctac agactttgag
     2461 caagtccaaa ctctcaccat taatataatg ttttggtaat aatcctataa cactgatgtt
     2521 acctgcttaa tccagcaata ccatgcctgt ctgctatgct tttttgatgc gtttagcgaa
     2581 atttctcaga agtgtgaatt aacgcactca tctaacactt tacttttcaa ggagtatttc
     2641 ctatgaacga gttagacggc atcaaacagt tcaccactgt cgtggcagac agcggcgata
     2701 ttgagtccat tcgccattat catccccagg atgccaccac caatccttcg ctgttactca
     2761 aggctgccgg attatcacaa tatgagcatt taatagacga tgctatcgcc tggggtaaaa
     2821 aaaatggcaa gacccaggaa caacaggtgg tcgcagcgtg tgacaaactg gcggtcaatt
     2881 tcggtgctga aatcctcaaa atcgtacccg gtcgcgtgtc aacagaagtt gatgcacgcc
     2941 tctcttttga taaagaaaag agtattgaga aggcgcgcca tctggtggac ttgtatcagc
     3001 aacaaggcgt tgagaaatca cgcattctga tcaaactggc ttcgacctgg gaaggaattc
     3061 gcgcggcaga agagctggaa aaagaaggta ttaactgcaa cctgacgctg ctgttttctt
     3121 ttgcacaggc gcgggcctgt gcagaagcgg gcgtttttct gatttcgccg tttgtcgggc
     3181 gtatttatga ctggtatcag gcacgcaagc cgatggaccc atatgtggtg gaagaagatc
     3241 cgggcgttaa atcggtgcgc aatatctacg actactataa gcaacaccac tatgaaacca
     3301 ttgtgatggg cgcgagcttc cgtcgcaccg aacaaatcct ctccttaacc ggctgcgatc
     3361 gactgactat cgcaccgaat ttgctgaagg agctgcagga aaaagtttcg ccagtggtac
     3421 gtaaattaat cccaccttct cagacgttcc cacgcccagc tcccatgagc gaagcggagt
     3481 tccgttggga gcacaatcag gatgcgatgg cggtagaaaa actgtctgaa ggcattcgtc
     3541 tgttcgccgt tgatcaacgc aaactggaag atcttcttgc cgccaaacta taaaccagcc
     3601 acggagtgtt atatgtcccg aaaagacctt gccaatgcga ttcgcgcact cagtatggat
     3661 gcggtacaaa aagccaattc tggtcatccc ggcgcgccga tgggcatggc tgatattgcc
     3721 gaagtgctgt ggaacgattt tcttaaacat aaccctaccg acccaacctg gtatgatcgc
     3781 gaccgcttta ttctttccaa cggtcacgcg tcgatgctgc tctacagttt gctgcatctg
     3841 accggttacg acctgccgct ggaagagctg aagaactttc gtcaactgca ttcgaaaacc
     3901 cctggccacc cggaaatcgg ctatacgccc ggagttgaaa ccaccaccgg tcctcttgga
     3961 caaggtttgg cgaacgccgt cgggctggcg atagcggaac gtacactggc ggcgcagttt
     4021 aaccagccgg atcatgaaat tgtcgatcac ttcacctatg tgtttatggg cgacggctgc
     4081 ctgatggaag gtatttccca cgaagtctgt tcgctggcgg gcacgctggg actgggcaag
     4141 ctgattggtt tttacgatca caacggtatt tcgattgatg gagaaaccga aggctggttt
     4201 accgacgata cggcaaaacg ttttgaagcc tatcactggc atgtgatcca tgaaatcgac
     4261 ggtcacgatc cgcaggcggt gaaggaagca atccttgaag cgcaaagcgt gaaagataag
     4321 ccgtcgctga ttatctgccg tacggtgatt ggctttggtt cgccgaataa agcaggtaag
     4381 gaagaggcgc acggcgcacc gctgggggaa gaagaagtgg cgctggcacg gcaaaaactg
     4441 ggctggcacc atccgccatt tgagatccct aaagatattt atcacgcttg ggatgcccgc
     4501 gaaaaaggcg aaaaagcgca gcagagctgg aatgagaagt ttgccgccta taaaaaggct
     4561 catccgcaac tggcagaaga gtttacccgt cggatgagcg gtggtttacc gaaggactgg
     4621 gagaaaacga ctcagaaata tatcaatgag ttgcaggcga atccggcgaa aatcgctacc
     4681 cgtaaggctt cgcaaaatac gcttaacgct tacgggccga tgctaccgga gctgctcggc
     4741 ggttcggcgg atctggctcc cagcaacctg accatctgga aaggttctgt ttcgctgaag
     4801 gaagatccgg cgggcaacta cattcactac ggggtgcgtg aatttggcat gaccgctatc
     4861 gccaacggca ttgcgcacca cggcggcttt gtgccgtata ccgcaacgtt cctgatgttt
     4921 gttgaatacg cccgtaacgc cgcgcggatg gcggcactga tgaaagcgcg gcagattatg
     4981 gtttataccc acgactcaat tggtctgggc gaagatggtc cgacgcacca ggctgttgag
     5041 caactggcca gcctgcgctt aacgccaaat ttcagcacct ggcgaccgtg cgatcaggtg
     5101 gaagcggcgg tgggctggaa gctggcggtt gagcgccaca acggaccgac ggcactgatt
     5161 ctctccaggc agaatctggc ccaggtggaa cgtacgccgg atcaggttaa agagattgct
     5221 cgtggcggtt atgtgctgaa agacagcggc ggtaagccag atattatttt gattgccacc
     5281 ggttcagaga tggaaatcac cctgcaagcg gcggagaaat tagcgggaga aggtcgcaat
     5341 gttcgcgtgg tttccctgcc ctcgaccgat attttcgacg cccaggatga ggaatatcgg
     5401 gagtcggtgt tgccttctaa cgttgcggct cgcgtggcgg tggaagcagg tattgccgat
     5461 tactggtaca agtatgttgg tctgaaaggg gcaattgtcg ggatgacggg ttatggggaa
     5521 tctgctccgg cggataagct gttcccgttc tttggcttta ccgccgagaa tattgtggca
     5581 aaagcgcata aggtgctggg agtaaaaggt gcctgatggt gatcgccgga tgctgattgc
     5641 cggatgcgac gctgacgcgt cttatccggc ctacagcgtc ttatccggcc tacatgtccc
     5701 gccattttgt ttaacgggtg atccacaacg tgggccaggc gtctggccca tgccagttat
     5761 cgcaggtggg ttctgcggcg taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc
     5821 gcgcctgaat gccacaatcg cttaatccgc gccctttcgc taaggtcacc agttcacgcg
     5881 atttctcatc aaatgttgcg ttcatcagtt ccagttcatt cgtctcctga ccgttgttga
     5941 acggcaaacg cagccggacc gggcgcgagg ccagcggctt tttacgcgac acaatccatg
     6001 ccaaatcaat ggtgttatag gcccctgcct cacagctaat catcatcagc gctttatcat
     6061 cagtcagcgc agtgacattc acctcacgac gcaatggatc aagcgagcag cgcagaccat
     6121 tcatccgcca gtttccataa tccagcaaat cgttgcgttc ttcgagtgag agtggcgtcg
     6181 gcgttgggtt aaccaccacg acctctttca gcgcaggcgc gggcggtacg ctgagcggcg
     6241 gttcgtcccc tttcttgatc cacgcggttt cactgccaac gcgcttttgc tgagcatcaa
     6301 taaacaacaa cgccgctttt aagtcactca gagaaatggt ctgattgccg tcgcgtaagg
     6361 tgatcgcctt cccttcctga atcatctgca aaaacgcggt gatggttgcc gtatcatcgg
     6421 ttactaataa ccatggtgaa atccgccact tgtcaccact taacgccaac ggctcgccat
     6481 ctaacagcag ccgtggcgca atttcccctt cagacgcatc cggcgacttc aacccgccgc
     6541 gctcaatacg taaaacggca tcggtatgcg ccccggcgct gcggctcagg gtcatcacca
     6601 gtccattatg atcgcccgta ttacgcgcca cgcagaagtt ttggttatta caggtgacct
     6661 gccagtcgga aaacgcccgt tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag
     6721 cgaaaaaaaa gagaaggaaa atgcgatagc gcatggacgg tacgacccca gaagttcaaa
     6781 caaacagcac aagtcgtatc ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga
     6841 ttgagccaag aataaacaac gaaatattat tgttattttt cagtctatta aatgtgacat
     6901 ttgcaaatag ttaagcaata acaccgtctt accgtcacgt atctcgccgg ttttgatcat
     6961 ctccagcgcc tggctgaacg gcagctcgag cacttcaata tcttcatctt cgacaccgcc
     7021 accggcgtta gcgcgctgat tgtcactgta ttcggcgata aaaaagtgga ttagctcagt
     7081 cacaccgcct ggcgacatat acagttcaaa taatttgcgc acttcgccca cttcatagcc
     7141 cgtctcttca atcgcttctt tgcgaatgca cacttccggt tcgtcgttat ccagcagccc
     7201 ggcgcaggtt tcaatcagct gcccgctttc attgccatta acccaggtag cgacacggaa
     7261 ctgacgaatc agaaccacgg tctttttctt cgcgttgtac aggaggatcg tcgcaccatt
     7321 accgcgatcg taaacttcac gtttatggcg gataacttcg ccatctttgc gcgtgagatc
     7381 gtaagtaatg ttgtgcaggg tgaaatagtt atcggagaga attttgtctt taatgagggt
     7441 gatttgttgc gtcataccga ctccacagcg cgaaatgaac aattatatta cgctgtgaag
     7501 tcgggtttgt ctgcgcaggc tatcagtgag atttgactga ttttaccccc agccagtcaa
     7561 taatcccctg tgccgcatga cgtccttctg ccattgcggt aaccaccaga tccgcaccac
     7621 gcacggcgtc gccaccggcg aagattttcg gattggtggt ctggtaacgg tactggcttt
     7681 ccacatccgc gatgatgcgg ccccatttgt ctaccgttac accgtgcgac tccagccacg
     7741 gcatcccgtg cggattgaag ccaaacgcca taatcaccgc gtcggctggc atgacaaatt
     7801 cactgccttc caccggcact ggacgccgac gcccctgggc atccggctcg ccaagacgcg
     7861 tgcgcaggaa acgaatcccg cagacgtgac cttgttcatt cagctcaagc gccaccggct
     7921 ggacgttaaa ttcgaagttg gccccctctt cgcgggcgtt cttcacctct ttcttcgagc
     7981 ctggcatgtt agcttcatca cgacgataag cgcaggtgac gttactcgcg ccgtggcgca
     8041 gcgcagtacg cacacagtcc atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc
     8101 cggcggtatt gataaacggc tcttccggta gctcttcgag ccccatcacc tgtttagtgt
     8161 tggcgatgag gaacggcagc gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg
     8221 ctttcatgga acggtaagtg cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg
     8281 aatccaaaga gacatcttta cccacttcac aattgagttc gaagtgaatc cccatcgcgc
     8341 tgaagatttc ccgacgacgt gccagcaggg atttatccag tttgaaagaa ggaatgccga
     8401 aagtgagcaa gccaccgatt tctggatggc gatcgtacac cgtcaccgcc acgccattgc
     8461 ggatcagaac atccgcacag gccagccctg ccggacctgc accgataatc gccacccgct
     8521 tgtccacttt ggtgacatgg cttaagtcag gacgccaacc tttcgccaac gcctgatctg
     8581 aaatgtagcg ttcaatgttg ccgatagtta ccgcaccgtg ctcatcgcga atagtacagg
     8641 caccttcaca caaacggtct tgcggacaaa cgcgtccggt aatttccggc agggtgttgg
     8701 tcaggtgaga aagctcgacg gcggcgtcga tgtttccggc tttcaccagt tcaatccact
     8761 gcggtatatg gttatgcagc gggcaggtcc attcacaaac gctgtgctcg ccgcatttaa
     8821 ggcagcgcga ggcttcccgt tgtgcctgat cggcacggaa tggcagataa atttcatcaa
     8881 aaccggtttt gcgcgcttca atcgccagtt tatccggctc gccacgcgcg ggcgttgcct
     8941 gcatttgttc gactttactc attaccggca tttcttgtgc agcggtactg gcatgccacg
     9001 gttgatgttc ctgacgtgcg gtacgcaagc ggcgggattt cgccatgcct gagagtgcgg
     9061 cgtcagtgac cagctgcagc gcgtccgccg ggcaattctc gacacaggca ggaccgtttt
     9121 cgcgccctgc acaaaggtcg catttatgcg ccgtggcttt aactttgccc gctgcgacgg
     9181 gtgtcaggac gatttgcatc gtaccaaacg gacaggccac cacgcaggat ttacagccaa
     9241 tacacttttg ctgattgacc tgaatgctgt catcaacgtg gctgattgcg ccattagggc
     9301 agctacgggc gcagggcgca tcttcacaat ggtgacaggt cactgcacta cgttgctgct
     9361 gatgtttgat aaccgtaatt cggggatgaa aatggtgttg gctcaggaca tgttgctcat
     9421 cattgtgagc catgacacaa gcgatttcac aagcatgaca acccagacat tgctgactgt
     9481 tggccataat aaaacgattc ataacgacct tcttttttgg ttgtaaaaac cttattcttt
     9541 atatgagtgt tgttattacc cgacttacag gggaatcggc aatgttcatg tgcccagaat
     9601 aagtaactat ttcgctataa actgtggcag atcaaataat cccatcacta actaaattgc
     9661 gtttcagcga actggaacat taatgatttt ttgtggagaa gacgcgtgtg attgttaaac
     9721 gacccgtctc ggccagtctg gcccgggcct ttttttacat tgtgctgctg tcgattcttt
     9781 ccacgggtat cgctctgcta actctggcga gcagtttgcg cgacgctgag gctatcaata
     9841 ttgccggatc gctgcgtatg cagagttacc gcctgggcta cgacttgcaa agtggcactc
     9901 cacaactcaa tgcacatcgc cagctgtttc agcaggcact gcattcaccg gtattaacca
     9961 acctcaacgt ctggtatgtg ccagaagcag taaaaactcg ctatgcgcat ctgaatgcca
    10021 actggctgga gatgaataat cggctcagca agggcgattt gccgtggtat caggccaata
    10081 ttaataatta tgttaatcag atagacctgt tcgtgctggc tttacagcac tacgctgaac
    10141 gcaaaatgct gctggtggtg gcgatttccc tggctggcgg tatcggtatt ttcacgctgg
    10201 tcttttttac tttgggccgc atacgccatc aggtggttgc cccgctgaat cagctggtta
    10261 ccgccagtca gcgtattgaa cacggacagt tcgactcgcc accgctggat accagcctgc
    10321 cgaatgagct tggtctactt gcaaaaacct ttaaccagat gtcgagcgag ctgcataaat
    10381 tgtaccgttc gctggaggcg tcagtagaag aaaagacccg cgatctccac gaggccaagc
    10441 gtcgtctgga ggtgttgtat caatgttcgc aggcgctgaa caccagccag attgatgtgc
    10501 attgtttccg ccatattttg cagattgttc gcgacaatga agcggctgaa tatctggagt
    10561 taaatgtcgg tgaaaactgg cggattagcg aagggcaacc aaacccggaa ttgccgatgc
    10621 agattttacc ggtgacaatg caagagacgg tttacggcga actgcactgg caaaatagtc
    10681 acgtttcatc atcagaaccg ctgcttaaca gcgtttcgtc gatgctggga cgcggtttgt
    10741 actttaatca ggcgcagaag cattttcagc agttattgtt gatggaagaa cgtgcaacca
    10801 tcgcccgcga attgcacgac tcgctggctc aggtactttc ttacttacgt atccagttga
    10861 cgttacttaa gcgttcgata ccggaagata acgccaccgc acaaagtatc atggccgatt
    10921 tttcccaggc gttgaatgat gcttatcggc agttacgcga gctgttgact acttttcgcc
    10981 tgacgctgca gcaggcggat ctcccctccg cgttgaggga aatgctggat acgttacaaa
    11041 atcaaaccag cgccaaactg accctcgact gccgtctgcc aaccctggca ctggatgcgc
    11101 aaatgcaggt gcatttgttg caaattattc gcgaagcggt gctgaatgcg atgaagcacg
    11161 ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc ggacggcaat cacacggttt
    11221 atatccgtga taacgggatt ggtatcggtg aaccgaaaga acccgaaggt cattatggtc
    11281 tgaatatcat gcgcgaacgc gcggaacggc taggtgggac gctgactttt tcacaacctt
    11341 ccggcggcgg cacgttagtg agtattagct ttcgctctgc ggagggtgag gaaagtcagt
    11401 tgatgtaatg cctcctactg accaaagaat acttgcagtt aaggttcagt ataaaagggc
    11461 atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt gccgctacag
    11521 tgaagcaagt caagcctaca acgatacgca gaaacacgag gtcctctttt aatggcgaat
    11581 ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg tctgacaggt
    11641 accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc accgaatgtg
    11701 cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac cgtgacccag
    11761 gttatcgagc aaaatatgac cggcctcgat aatctcatgt atatgtcatc tcagagcagt
    11821 ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc ggacgaagcc
    11881 gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa
    11941 aatcagggcg tgactgtgcg taaaaccggc gataccaaca ttctgaccat tgccttcgtc
    12001 tctaccgatg gttcgatgga taaacaggat attgctgatt atgttgccag taatattcag
    12061 gacccgttaa gccgcgtaaa tggcgtcggg gatatcgatg cctatggttc gcaatattcc
    12121 atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacagc taaagatgtc
    12181 actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg tggtacacct
    12241 tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct gcaaacacca
    12301 gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaagt aaggctcggc
    12361 gatgtcgcca ccgccgaaat gggggcggag aaatacgatt atcttagccg cttcaatggt
    12421 aagccagcct ccgggttggg ggtaaaactg gcctccggcg cgaacgaaat ggcgacagcg
    12481 gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg actggaatac
    12541 aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg
    12601 ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca aaacttccgc
    12661 gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt ctccgtactt
    12721 tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct ggcgatcggt
    12781 ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa
    12841 ggactcactc ctcgcgaagc cacgcgtaaa tcgatggggc agatccaggg ggcactggtc
    12901 gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc
    12961 ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct gtcagtactg
    13021 gtagcgatga tcctcactcc ggctctgtgt gccacactac ttaagccact gaaaaaaggt
    13081 gagcatcacg ggcaaaaagg cttttttgcc tggtttaacc agatgtttaa ccgcaacgcc
    13141 gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt
    13201 tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac gtcgttctta
    13261 ccgctggaag accgtggcat gtttactacc tcggtacaat tgcccagcgg ttcaacccaa
    13321 caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca tgaaaaagac
    13381 aacatcatgt cggtgtttgc cactgttggt tctggccctg ggggtaacgg gcaaaacgtg
    13441 gcgcgaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac cggcacctcg
    13501 tttgccatta tcgagcgtgc aacaaaggcg tttaaccaaa ttaaagaagc tcgcgttatc
    13561 gccagcagcc cgccagcaat tagcggtctt ggtagttctg caggttttga tatggagttg
    13621 caggaccacg ctggagcagg tcacgatgcg ctgatggcag cacgtaacca gttgctggcg
    13681 ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga tgacagtccg
    13741 cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttgc tatcgacgat
    13801 attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt tatggatcgc
    13861 ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct gccagatgac
    13921 atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc tgctttcgcg
    13981 acctcacgct gggagacagg ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt
    14041 gaaattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga tattatggaa
    14101 tcgttagtga agcagctacc aaacggcttt ggtctggagt ggacggcgat gtcgtatcag
    14161 gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct ggtggtattc
    14221 ctgtgtctgg ctgcattgta tgaaagctgg tcggtaccgt tctcggtaat gctggtcgtg
    14281 ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga aaacgacgtt
    14341 tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc
    14401 gtcgagtttg ctaacgagat gaaccaaaaa ggccacgacc tgtttgaagc gacgctccac
    14461 gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat cttcggcgta
    14521 ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt gggtactggc
    14581 gtaatgggcg ggatgatttc agccactatt ctggctattt acttcgtgcc gctgttcttt
    14641 gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg
    14701 acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag cgggttcttg
    14761 ctgtttgtac tttgtctcag gaattactta cgaagcataa cttcaataaa gtctttccag
    14821 ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat tctacggaaa
    14881 caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc cgggggacaa
    14941 accttatgta aatactatgg tcctgcagtg atgatttgta tgtgatacac agcaacattt
    15001 cgagatattc atacggcatc taatactgat tta
//
LOCUS       DAZFBR010000111        14931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFBR010000111 DAZFBR010000000
VERSION     DAZFBR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.111"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            139..381
                     /gene="yjbE"
                     /locus_tag="ACHJZ2_003825"
     CDS             139..381
                     /gene="yjbE"
                     /locus_tag="ACHJZ2_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO4569855.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            495..1133
                     /gene="yjbF"
                     /locus_tag="ACHJZ2_003826"
     CDS             495..1133
                     /gene="yjbF"
                     /locus_tag="ACHJZ2_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO4569856.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1130..1867
                     /gene="yjbG"
                     /locus_tag="ACHJZ2_003827"
     CDS             1130..1867
                     /gene="yjbG"
                     /locus_tag="ACHJZ2_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO4569857.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALHQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            1867..3963
                     /gene="yjbH"
                     /locus_tag="ACHJZ2_003828"
     CDS             1867..3963
                     /gene="yjbH"
                     /locus_tag="ACHJZ2_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4569858.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERANTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4010..4288)
                     /locus_tag="ACHJZ2_003829"
     CDS             complement(4010..4288)
                     /locus_tag="ACHJZ2_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569859.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4289..4432
                     /locus_tag="ACHJZ2_003830"
     CDS             4289..4432
                     /locus_tag="ACHJZ2_003830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569860.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4558..4968
                     /gene="psiE"
                     /locus_tag="ACHJZ2_003831"
     CDS             4558..4968
                     /gene="psiE"
                     /locus_tag="ACHJZ2_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO4569861.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(5012..6487)
                     /gene="xylE"
                     /locus_tag="ACHJZ2_003832"
     CDS             complement(5012..6487)
                     /gene="xylE"
                     /locus_tag="ACHJZ2_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO4569862.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6859..7749)
                     /gene="malG"
                     /locus_tag="ACHJZ2_003833"
     CDS             complement(6859..7749)
                     /gene="malG"
                     /locus_tag="ACHJZ2_003833"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO4569863.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7764..9308)
                     /gene="malF"
                     /locus_tag="ACHJZ2_003834"
     CDS             complement(7764..9308)
                     /gene="malF"
                     /locus_tag="ACHJZ2_003834"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO4569864.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9463..10653)
                     /gene="malE"
                     /locus_tag="ACHJZ2_003835"
     CDS             complement(9463..10653)
                     /gene="malE"
                     /locus_tag="ACHJZ2_003835"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO4569865.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11018..12133
                     /gene="malK"
                     /locus_tag="ACHJZ2_003836"
     CDS             11018..12133
                     /gene="malK"
                     /locus_tag="ACHJZ2_003836"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO4569866.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12205..13545
                     /gene="lamB"
                     /locus_tag="ACHJZ2_003837"
     CDS             12205..13545
                     /gene="lamB"
                     /locus_tag="ACHJZ2_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO4569867.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13696..14616
                     /gene="malM"
                     /locus_tag="ACHJZ2_003838"
     CDS             13696..14616
                     /gene="malM"
                     /locus_tag="ACHJZ2_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO4569868.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14845..>14931
                     /locus_tag="ACHJZ2_003839"
     CDS             14845..>14931
                     /locus_tag="ACHJZ2_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569869.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFD"
BASE COUNT         3683 a         3839 c         3936 g         3473 t
ORIGIN      
        1 ttgatattca ttccaattat cctaaaaccc cgtctctaat tccccgcgcc gtaattcgca
       61 tgctttagtt gtgtatactc gatcccgccc gaaatgtttt tgggtaaatc tccattcatt
      121 caatgaaggg aaattgttat gaaaaaagtt ctgtatggca tttttgccat atctgcgctt
      181 gcggcgactt ctgcgtgggc tgcacctgta caggtgggcg aagcggcagg gtcggcagca
      241 acgtcggttt cggcggggag ttcctccgcg accagcgtca gcaccgtaag ctcggcggtg
      301 ggtgtcgcgc tcgcggcaac cggtggcggt gatggttcta ataccgggac caccacaacc
      361 acgaccacca gtacccagta ataaagtatg tatccccaaa ataattcgag tcattgcatc
      421 tgtggctaga agtatgaagg gatttaccat aaccacactc cggtgtggtt attctgcccc
      481 tctggagaag agtcgtgaag cgacctgcat tcattcttat ctgcctgcta ttacaggcct
      541 gttcagccac gactaaagag ctgggcaatt cactgtggga cagtctgttc ggcacgccag
      601 gcgtacagct gacggacgat gatattcaaa atatgcccta cgccagccag tacatgcagc
      661 ttaatggtgg gccgcagttg tttgtggtgc tggccttcgc tgaagacgga caacaaaaat
      721 gggtcactca ggatcaggcc actctcgtca cacaacatgg tcgtctggtg aagactttgc
      781 ttggcggcga caacctgatt gaagtgaata accttgccgc cgacccgctg attaaacccg
      841 cacaaatcat tgatggcgca acctggatgc gcacgatggg ctggaccgag taccagcagg
      901 tacgctacgc caccgcacgc tcagtcttca aatgggatgg caccggtacc gttaaggtcg
      961 gcagcgatga aaccgcggtt cgcgtgctgg acgaagaagt ctccaccgac caggcgcgct
     1021 ggcataaccg ctattggatc gacagcggag ggcaaattcg ccagtcggaa cagtatctcg
     1081 gcgcggatta tttcccggtg aaaaccactc tcatcaaggc ggcaaaacaa tgattaaaca
     1141 aactattgtc gcgttgcttt tgagtgtggg agcgtcatcg gtcttcgcgg caggaaccgt
     1201 caaggtgttc agcaatggca gcagtgaggc caaaacgcta acgggcgcag agcatttaat
     1261 cgatctggta ggccaaccgc ggctggcaaa cagctggtgg cctggtgcgg tgattagcga
     1321 agagctggca acggcggcag cattgcgtca gcagcaggcg ttgctgacac ggctggcaga
     1381 acagggcgca gattccagcg ccgacgatgc cgctgcgatt aacgccttac accagcaaat
     1441 tcaggcgttg aaggtgacgg gcaggcaaaa aatcaatctt gatcccgata tcgtccgcgt
     1501 tgctgaacgc gctaacccgc cgttgcaggg caactacacg ctgtgggtcg gaccaccacc
     1561 gtccacggtc acgttgttcg ggcttatcag ccgtcctggc aatcagccat tcactcccgg
     1621 tcgcgacgtg gcgagctatc tctccgggca aagtctgctt agcggtgcgg atcgtagcta
     1681 cgcgtgggtg gtttacccgg acggacgcac acaaaaagcg ccggtggctt actggaacaa
     1741 gcgtcacgta gagccgatgc ccggcagcat tatttatgtt ggcctcgcgg actccgtctg
     1801 gagtgagatg cctgatgccc ttaacgccga cattcttcag actctgacgc agcggatacc
     1861 tcaataatga aaaaaagaca tctgcttagc ttactggcgc tgggcattag cacagcttgc
     1921 tacggcgaaa catatcctgc gcccattggt ccgtcgcagt cggatttcgg tggcgtagga
     1981 ttattacaaa cgcccacggc tcgcatggcg cgggaagggg agttgagtct gaactatcgc
     2041 gataacgatc agtaccgtta ttactcagct tcagtgcaac tcttcccgtg gctggaaaca
     2101 acgctgcgct acaccgacgt gcgcacccgg cagtacagca gcgtcgaagc gttctctggc
     2161 gatcaaacgt ataaagataa agccttcgat ctcaaactgc gtttgtggga agagagttac
     2221 tggctgccgc aagtggcggt tggtgcgcgg gatatcggcg gtacggggct gtttgatgcg
     2281 gaatatcttg ttgccagcaa agcctggggg ccgttcgatt ttacgcttgg cctgggctgg
     2341 ggatatttgg gcacaagcgg taatgtgaaa aatccgctct gttcagccag tgataaatat
     2401 tgctatcgcg ataacagcta caaacaggcg ggatctatcg acggtagcca gatgttccac
     2461 ggtcctgcct cactgtttgg cggcgtggaa taccagacgc cctggcaacc gctgcgcctg
     2521 aaactggagt atgaaggcaa taattatcag caggattttg ccggtaagct ggagcaaaaa
     2581 agtaagttta acgtcggggc gatttatcgc gttaccgatt gggccgacgt taaccttagc
     2641 tatgaacgtg ccaacacctt tatgtttggc gtcacgctgc gcaccaactt taacgatctg
     2701 cgcccgtctt acaacgataa cgcccgcccg caatatcaac cgcagccgca ggatgccatt
     2761 ttgcagcatt cggtggtggc gaatcagtta acgctgttga aatacaatgc cggacttgcc
     2821 gatccgcaga tccaggcgaa aggcgatacg ctgtatgtta ccggcgagca ggtgaaatat
     2881 cgtgattcgc gcgaagggat cgttcgtgct aatcggatcg tgatgaatga tctgccggat
     2941 gggatcaaga cgatccgcgt tacggaaaat cgccttaaca tgccgcaggt gacgacggaa
     3001 acggatgtcg ccagcctgaa aaatcatctc gccggagagc cgttgggcca cgaaacgaag
     3061 ctggcgcaaa aacgcgtcga gccagtggtt ccgaagtcca ccgagcaggg ctggtatatc
     3121 gacaaatcgc gctttgattt ccatatcgat ccggtgctga accagtcggt cggtggcccg
     3181 gaaaactttt acatgtatca gctgggcgtg atgggaacgg cagatttgtg gctgacggac
     3241 catctgctga ccaccggcag cctgtttgca aatcttgcca acaactacga caagtttaac
     3301 tacaccaatc cgccgcagga ctcgcactta ccgcgcgtgc gtacccatgt gcgcgagtat
     3361 gtgcagaacg atgtctatgt gaataacctg caagccaact acttccagca tctgggcaac
     3421 ggcttctacg gtcaggtcta cggaggttat ctcgaaacca tgtttggcgg tgcgggggca
     3481 gaagtgttgt atcgcccgct ggacagcaac tgggcgtttg gtctggatgc caactacgtt
     3541 aaacagcgcg actggcgtag tgcaaaggat atgatgaaat tcaccgacta cagcgtgaaa
     3601 accggacatc tgaccgccta ctggacgcca tctttcgctc aggatgtgtt agttaaagcc
     3661 agcgtcgggc agtatctggc aggggataaa ggcggcacgc tggagatcgc taaacgcttt
     3721 gacagcggcg tggtggtggg cggctatgcc acgatcacca acgtttcgaa agaggagtac
     3781 ggcgaagggg acttcaccaa aggcgtgtat gtctctgtac cgttggatct cttctcgtct
     3841 ggcccgacac gcagccgtgc ggcgattggc tggacgccgc tgacgcgtga cggtggtcag
     3901 caacttgggc gtaagttcca gttgtatgac atgaccagcg accgtagcgt caatttccgc
     3961 taagtcatgg gaaaggtgcc agttttcgca atcactggca cctttattct taatggcctg
     4021 ttgagccatc aggaacgcag ttcacccccg gtttgcgatc tggtgtgtaa aggcgaaaac
     4081 attctgtctt cctgtccgtc gatatcggtt gcaggtctgg ttgcctgagc gactgggcca
     4141 tcattgggct ggagacattg ctttgcggta ggtcggcttt atcagcagta ccgagcggac
     4201 cagcataagc aggaagagca gagactgata acatcaaagc agcaaaataa ggcttcattt
     4261 ttaccacctt tatcaggtta cgtttcattt gttccagagg aacattgtcg atttttcgcg
     4321 cattgctggt ggctgggaat cacctgaatg ggtgattttt gaattaccgg ctttggtgcg
     4381 gtttgtcttg ccggatgcgc cgccaggcgc ggcttatccg gcctacgggt aggtatatcc
     4441 ggctttggtg gaggcgcgct ccaaatccag gttgaacaaa acatacacaa aaaatataga
     4501 tctccgtcac atttttgcgt tatacaggaa gctcgccact gtgaaggagg tactgctatg
     4561 acgtcactct ctcgtccgcg cgtggagttt atctccacca ttttgcagac cgtactcaat
     4621 cttggcctgc tgtgcctcgg ccttattttg gttgtcttcc tcggcaaaga aacggtgcat
     4681 ctggctgatg tgctgttcgc gccagaacaa gccagcaaat atgagctggt agaaggactg
     4741 gtggtttact ttctctattt cgaatttatc gcgctgattg tgaagtactt tcagtccggt
     4801 tttcacttcc cgctacgtta ctttgtctat attgggatca ccgccattgt gcgcttgatc
     4861 atcgtcgatc ataaatcgcc actggatgtg ttgatctact cggctgcgat cctgttactg
     4921 gtggtcaccc tgtggctgtg taattcgaaa cggctgaagc gggagtaaaa agacagcacg
     4981 ccgaactggc gcggcgtgct ggacaggaag attacagcgt agcagtttgt tgtgttttct
     5041 tcgtttccgg ttcccagagg gcttccagct cctcaagggt tttacctttg gtttccggga
     5101 caaatttcca cataaacagt gctgccagaa cgcccataca accgtaaatc cagtaggaga
     5161 aaccgttgtg gaaatgggcc accagccagg agtttttgtc catcatcggg aaggtccagg
     5221 agacgaagta gttcgccagc cactgggccg ccaccgcgat tgccagcgct ttacctcgaa
     5281 tagcattcgg gaagatttcc gacagcagta cccagcatac cggaccccag gacatggcaa
     5341 aggcggcaac atagaacagc atcgacagta gcgccacaat acccggtgcc tgagtgtaaa
     5401 acgcggtacc gaggctaaac ataccgattg ccattccgag tgcgccgata atttgcagtg
     5461 gcttacgacc aaatttatcc accgtcataa ttgccagaac ggtgaaggtg aggttgataa
     5521 ctccgacaat aatggtctgc aacagcgcga tatccgtgct ggcccccagc gttttgaaca
     5581 cttccggcgc gtagtacagc accacattga tgccgacaaa ttgctggaag atggagagca
     5641 taacgccgat gacaatcacg cccacgccaa acatcagcag acgaccaccg gttttgcggc
     5701 catgatccag ggagtgttta atttcctgta ctgcctgagt tgcaagcgtg ttgcccataa
     5761 ttttgcgcag gataccttcc gcctgttctt gcttgccgcg tgacatcagc cagcgaggac
     5821 tttctggcac ggtatacagc agcattaaga acagcagtgc agggatacat tccgaggcaa
     5881 acatataacg ccagccgtca gtattcagcc agctggcatc accggaacgc gcaataaaat
     5941 agtttacgca gtaaactaaa agctgcccga aaataatcgc aaactggtta aatgaaacca
     6001 gtttcccgcg aatatgagca ggagcaagtt ccgcaatata cattggtgaa agcattgagg
     6061 ctaaaccaac gccaataccg ccaataatac gataaataac aaattccggg acataacctg
     6121 ccagataaac aggcactgtg ttgtccgggt ttatagaggt aaaaccaagt tctggccagg
     6181 cagaacctac accagaaata aaaaacagga cagcagcaat cttaagtgaa tcacgacgac
     6241 cgaagcggtt actgcaataa ccaccgaggg caccgccgat gatgcaacca atcagagcgc
     6301 tggccacgca aaaccctaac agggagttgg cagcggattc acttaagttt tgtggagcaa
     6361 caaagacggt attgagtgac tcaacagtac cggaaataac ggcggtgtcg tagccaaata
     6421 ataaaccacc taatgtagcg actaaggtaa tcgaaaatat ataactggaa ttatactggg
     6481 tattcattca gacctgcctt agaccattct gatcttttat cagtgcattg atgttggaat
     6541 tgtcaggcag ttaaggcaat tatcattttt tacaatgcgt ttacgtaatg tcttttctgt
     6601 gatcttaatt gtgataatta tccaaaattg ataaaaacaa tactattgcc gtgactcaga
     6661 gcacgaaaga gaattatcgt aagtgggaaa acaaataacg taaaaataat aagctctatg
     6721 atggaaatat taaccggcga acgattcaga ttgcagacga aagaaaaaaa ggcgctccgt
     6781 ggagcgccga ataacagtca caagttggga taacgtaagt tgagggtgca gcggcataac
     6841 attggcagaa caacatcttt aacctttcac accacctgcc gtcaggccgt tgaccagcca
     6901 gcgttgagcc agcaagaaga cgatggtgat cggtaatgca gacatcacgg cagcggcggc
     6961 aaagtcaccc cacaggtagt tttgcggatt gaggtattgc tgcatcccca cggccagggt
     7021 gtagctgttt acgtcacgca gtaacagcga cgcgaccgga acttcagtaa tggcagcgat
     7081 aaacgacagg atgaatacca ccgccagaat cggtactgac agcggcaaca ggacaaggcg
     7141 gaaggcctgc cacggtgtcg caccatccag cgcagccgct tcttccagcg aactgtcgat
     7201 ggtttcgaaa tagcctttga tggtccagac gtgcagcgca atcccaccca gatacgcgaa
     7261 aattacgccg ccgtgagtat tcaggccaat gaatggaatg tactcaccca gacgatcaaa
     7321 caacgcatac aacgcgacca gtgaaagtac tgccgggaac atctggaaaa tcagcattcc
     7381 tttcagcagc gtcgctttgc ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt
     7441 ggagagcgcc acaatgccaa tcgcggaaat cccggcgacc tttaccgagt tccacagcca
     7501 cagcagcacc gggaatggcg gtggcgtaat gcgaccatca gcctgttcca cgctaaaacc
     7561 taatgccagt ttccagtgat cccaggagat ttgctccggg atcaggctgc cggtcgcaaa
     7621 gttcccctgg cgcagtgaga tagcgacgac catcagcagc gggaacataa tcgccgcgat
     7681 aaaaagtagc agtagcaggt gagtaataaa caaacgtgct ttttgcgatt tcggttggac
     7741 cattgccatt tttgttatct cccttaatca aacttcattc gcgtggcttt caggttcact
     7801 atcgccagcg cacccaccag caggaagatc agcgtggcaa ttgctgctgc cagaccgaag
     7861 tcctgacccc cgccgccttc aaaagcgatg cggtaggtgt agttaacaag caggtcggta
     7921 taaccggctg gcgtggtcgt gccaagacga tccgggccgc cgttggttaa cagttgaatc
     7981 agcacgaagt tgttaaagtt aaaggcgaag ctggcgatca tcagcggcgt cagcggttta
     8041 atcagcagcg gcagcgtaat cttaaagaag ttctggaacg gacctgcgcc atccattgct
     8101 gaggcttcat acaaatcgtc cggaatcgct ttcagcaagc ccatgcagag gatcatcatg
     8161 tacggatagc ccagccaggt attgacgata atcagcatcg ttcgggcggt ggtcggatcg
     8221 ctgaaccagg cgggcttcac gccaaacagc gcgctcaaca tcatgttgat ctcaccgaag
     8281 ctctggttaa acaacccttt gaaaatcaag attgaaatga acgatggcac cgcgtagggc
     8341 agaatcagca ggacgcgata gaccgctttg ccgcgcaacg cttcccactg caccagacac
     8401 gccagaacca tgccgactgc caccgttaaa aagacagtga tcagcgagaa caccacggtc
     8461 cagacgaaaa tggcgaggaa cggtttctga atgccttcgt cggtgaagac gcgagtaaag
     8521 tttttccagc cggtggtcac ggtgtaaccg gggcttagct tttcatcacc ccagttgccg
     8581 tcggcggtaa tggactggta aaagccaatt tggttattcg gacgatattt cacgccgctc
     8641 tgattattcg tcaacgtgcc gtcaccgtcg agtgtgtaga gcggctgcgt gccagaaaac
     8701 tggcgcaggg agctcatcat cactttgttg ccatccggca gaatggcggt aatgtcactc
     8761 agcgcctgac gattctgggt aatcacgcgt agatttgcgc gttcgccttc gggctgggcg
     8821 gtcgtttctt tcagttgcag tttttgctct ctgccaaatt taaaagcgtc ggagaggtaa
     8881 tttttgccgg tttcgccgtc gctgagcgcc agttgccact catcgcccgc cgggtaaaga
     8941 ccaaagttat aggttttgcc tgcttgccag gagcgatcta acaacacttc ctgtgcacgt
     9001 tcaaaagtca gctggttagt gctgctgtag ttggtgaagg caatggcgat ggtgcagacc
     9061 agagggaaga ggacgaataa tcccattcca gccattcccg ggtaaacata gcgccaggcg
     9121 taggctttac gattggcgaa aatatacagc cccgctgaac tcaatatcag cgtggtaatg
     9181 gcgaacaggt attccccttg tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg
     9241 agcagaccta acactgacca tttcagcgcg tcgctttgcc accaatgttt ctttttaatg
     9301 acatccatgg ggttcttcct cattccagga cggataaggc ttttcatgcc ttatccgaca
     9361 acaactgcct gatgcgacgc tgacgcgtct tatcaggcct acatacgttt cggttttgta
     9421 ggccggacaa ggcgttcacg ccgcatccgg catttcacag cattacttgg tgatacgagt
     9481 ctgcgcgtct ttcagggctt catcgacagt ctgacgaccg ctggcggcgt tgatcaccgc
     9541 agtacgcacg gcataccaga aagcggacat ctgcgggatg ttcggcatga tttcaccttt
     9601 ctgggcgttt tccatggtgg ctgcaatacg tggatctttc gccaactctt cctcgtaaga
     9661 cttcagcgct acggcaccca gcggtttgtc tttattaacc gcttccagac cttcgtcagt
     9721 cagcagatag ttttcgagga actctttcgc cagctctttg ttcggactgg cggcgttaat
     9781 accggcgctc agcacgccaa cgaacggttt agatggttga cccttgaagg tcggcagtac
     9841 cgttacacca taattcactt tgctggtgtc gatgttggac catgcccacg ggccgttgat
     9901 agtcatcgct gtttcgcctt tattaaaggc agcttctgcg atggagtaat cggtgtctgc
     9961 attcatgtgt ttgtttttaa tcaggtcaac caggaaggtc agacccgctt tcgcgccagc
    10021 gttatccacg cccacgtctt taatgtcgta cttgccgttt tcatacttga acgcataacc
    10081 cccgtcagca gcaatcagcg gccaggtgaa gtacggttct tgcaggttga acatcagcgc
    10141 gctcttacct ttcgctttca gttctttatc cagcgccggg atctcttccc aggtttttgg
    10201 cgggttcggt agcagatctt tgttataaat cagcgataac gcttcaacag cgatcgggta
    10261 agcaatcagc ttgccgttgt aacgtacggc atcccaggta aacggataca gcttgtcctg
    10321 gaacgctttg tccggggtga tttcagccaa caggccagat tgagcgtagc caccaaagcg
    10381 gtcgtgcgcc cagaagataa tgtcagggcc atcgccagtt gccgcaacct gagggaattt
    10441 ctcttccagt ttatccggat gctcaacggt gactttaatt ccggtatctt tctcgaattt
    10501 cttaccgact tcagcgagac cgttatagcc tttatcgccg ttaatccaga ttaccagttt
    10561 accttcttcg attttggcga gagccgaggc ggaaaacatc atcgtcgtta atgcggataa
    10621 tgcgaggatg cgtgcacctg tttttatttt cataatctat ggtccttgtt ggtgaagtgc
    10681 tcgtgaaaac acctaaacgg actctagttt ctttatacgg caacctcttt ccatcctcct
    10741 tgcccctacg ccccaccgtt gctttgtgtg atctctgtta cagaattggc agtaatgtgg
    10801 agatgcgcac ataaaatcgc cacgattttt gcaagcaaca tcacgaaatt ccttacatga
    10861 cctcggttta gttcacagaa gccgcgttct catcctcccg cctcctcccc cataaaaaag
    10921 ctagggggtg gaggatttaa gccatctcct gatgacgcat agtcagccca tcatgaatgt
    10981 tgctgtcgat gacaggttgt tacaaaggga gaagggcatg gcgagcgtac agctgcaaaa
    11041 tgtaacgaaa gcctggggcg aggtcgtggt atcgaaagat atcaatctcg atatccatga
    11101 aggtgaattc gtggtgtttg tcggaccgtc tggctgcggt aaatcgactt tactgcgcat
    11161 gattgccggg cttgagacga tcaccagcgg cgacctgttc atcggtgaga aacggatgaa
    11221 tgacactccg ccagcagaac gtggcgttgg tatggtgttt cagtcctacg cgctctatcc
    11281 ccacctgtca gtagcagaaa acatgtcatt tggcctgaaa ctggcaggcg caaaaaaaga
    11341 ggtgattaac caacgcgtca accaggtggc ggaagtgcta caactggcgc atttgctgga
    11401 tcgcaaaccg aaagcgctct ccggtggtca gcgtcagcgt gtggcgattg gccgtacgct
    11461 ggtggccgag ccaagcgtat ttttgctcga tgaaccgctc tccaacctcg atgctgcact
    11521 gcgtgtgcaa atgcgtatcg aaatctcccg tctgcataaa cgcctgggcc gcacaatgat
    11581 ttacgtcacc cacgatcagg tcgaagcgat gacgctggcc gacaaaatcg tggtgctgga
    11641 cgccggtcgc gtggcgcagg ttgggaaacc gctggagctg taccactatc cggcagaccg
    11701 ttttgtcgcc ggatttatcg gttcgccaaa gatgaacttc ctgccggtaa aagtgaccgc
    11761 caccgcaatc gatcaagtgc aggtggagct gccgatgcca aatcgtcagc aagtctggct
    11821 gccagttgaa agccgtgatg tccaggttgg agccaatatg tcgctgggta ttcgcccgga
    11881 acatctactg ccgagtgata tcgctgacgt catccttgag ggtgaagttc aggtcgtcga
    11941 gcaactcggc aacgaaaccc aaatccatat ccagatccct tccattcgtc aaaacctggt
    12001 gtaccgccag aacgacgtgg tgttggtaga agaaggtgcc acattcgcta tcggcctgcc
    12061 gccagagcgt tgccatctgt tccgtgagga tggcactgca tgtcgtcgac tgcataagga
    12121 gccgggcgtt taagcacccc acaaaacaca caaagcctgt cacaggtgat gtgaaaaaag
    12181 aaaagcaatg actcaggaga tagaatgatg attactctgc gcaaacttcc tctggcggtt
    12241 gccgtcgcag cgggcgtaat gtctgctcag gcaatggctg ttgatttcca cggctatgca
    12301 cgttccggta ttggctggac aggtagcggc ggtgaacaac agtgtttcca gactaccggt
    12361 gctcaaagta aataccgtct tggcaacgaa tgtgaaactt atgctgaatt aaaattgggt
    12421 caggaagtgt ggaaagaggg cgataagagc ttctatttcg acactaacgt ggcctattcc
    12481 gtcgcgcaac agaatgactg ggaagctacc gacccggcct tccgtgaagc aaacgtgcag
    12541 ggtaaaaacc tgatcgaatg gctgccaggc tccaccatct gggcaggtaa gcgcttctac
    12601 caacgtcatg acgttcatat gatcgacttc tactactggg atatttctgg tcctggtgcc
    12661 ggtctggaaa acatcgatgt tggcttcggt aaactctctc tggcagcaac ccgctcctct
    12721 gaagcaggtg gttcttcctc tttcgccagc aacaatattt atgactatac caacgaaacc
    12781 gcgaacgacg ttttcgatgt gcgtttagcg cagatggaag tcaacccggg cggcacatta
    12841 gaactgggtg tcgactacgg tcgtgccaac ctgcgtgata actatcgtct ggttgatggc
    12901 gcatcgaaag acggctggtt attcactgct gaacatactc agagtgtcct gaagggcttt
    12961 aacaagtttg ttgttcagta cgctactgac tcgatgacct cgcagggtaa aggtctgtcg
    13021 cagggttctg gcgttgcatt tgataacgaa aaatttgcct acaatatcaa caacaacggt
    13081 cacatgctgc gtatcctcga ccacggtgcg atctccatgg gcgacaactg ggacatgatg
    13141 tacgtgggta tgtaccagga tatcaactgg gataacgaca acggcaccaa gtggtggacc
    13201 gtcggtattc gcccgatgta caagtggacg ccaatcatga gcaccgtgat ggaaatcggc
    13261 tacgacaacg tcgaatccca gcgcaccggc gacaagaaca atcagtacaa aattaccctc
    13321 gcacaacaat ggcaggctgg cgacagcatc tggtcacgcc cggctattcg tgtcttcgca
    13381 acctacgcca agtgggatga gaaatggggt tacgactaca acggcgatag caaggttaac
    13441 ccgaactacg gcaaagccgt tcctgctgat ttcaacggcg gcagcttcgg tcgtggcgac
    13501 agcgacgagt ggaccttcgg tgcccagatg gaaatctggt ggtaatagca aaacctgggc
    13561 cggataaggc gtttacgccg catccggcat aaaacaggtt gtcattatct gaaaggggcg
    13621 aaagcccctc tgattatcgg gtttagcgcg ctattgcctg gctaccgctg agctccagat
    13681 tttgaggtga aaacaatgaa aatgaataaa agtctcatcg ccctctgttt atcagcaggg
    13741 ttactggcaa gcgcgcctgg aatcagcctt gccgatgtta actacgtacc gcaaaacacc
    13801 agcgacgcgc cagccattcc atctgccgcg ctgcaacaac tcacctggac accggtcgat
    13861 caatctaaaa cccagaccac ccaactggcg accggcggcc aacaactgaa cgttcccggc
    13921 atcagtggtc cggttgctgc gtacagcgtc ccggcaaaca ttggcgaact gaccctgacg
    13981 ctgaccagcg aagtgaacaa acaaaccagc gtttttgcgc cgaacgtgct gattcttgat
    14041 cagaacatga ccccatcagc cttcttcccc agcagttatt tcacctacca ggaaccaggc
    14101 gtgatgagtg cagatcggct ggaaggcgtt atgcgcctga caccggcgtt ggggcagcaa
    14161 aaactttatg ttctggtctt taccacggaa aaagatctcc agcagacgac ccaactgctc
    14221 gacccggcta aagcctatgc caaaggcgtt ggtaactcga tcccggatat tcccgatccg
    14281 gttgctcgtc ataccaccga tggcttactg aaactgaaag tgaaaacgaa ctccagctcc
    14341 agcgtgttgg tcgggccttt atttggttct tccgctccag ctccggttac ggtaggcaac
    14401 acggcggcac cagctgtggc tgcacccgct ccggtgccgg tgaagaaaag cgagccgatg
    14461 ctcaacgaca cggaaagtta ttttaatacc gcgatcaaaa acgctgtcgc gaaaggtgat
    14521 gttgataagg cgttaaaact gcttgatgaa gctgaacgcc tgggatcgac atctgcccgt
    14581 tccaccttta tcagcagtgt aaaaggcaag gggtaattac gccccacagt gctgattttg
    14641 caacaactgg tgcgtctcct ggcgcacctt tttttatgct tcattccagg gatatgagcg
    14701 attttttata gtaactcact tcttcttcac taagaatatc cattatctca atgcattatc
    14761 agagattctt ttcctttcgc cggtagtgtc tggacgctca ggctactttt tccagttatt
    14821 tcatttctgt aatgcagagg ttttatgtta ggtcatatct caaagtttga cggcaataac
    14881 tctttgataa aacatggtgt ggtgcaagga aataatatag tagattttga t
//
LOCUS       DAZFBR010000112        14925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFBR010000112 DAZFBR010000000
VERSION     DAZFBR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.112"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            78..347
                     /locus_tag="ACHJZ2_003840"
     CDS             78..347
                     /locus_tag="ACHJZ2_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4569870.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            316..1434
                     /locus_tag="ACHJZ2_003841"
     CDS             316..1434
                     /locus_tag="ACHJZ2_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4569871.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1601..2395
                     /gene="potC"
                     /locus_tag="ACHJZ2_003842"
     CDS             1601..2395
                     /gene="potC"
                     /locus_tag="ACHJZ2_003842"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO4569872.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2392..3438
                     /gene="potD"
                     /locus_tag="ACHJZ2_003843"
     CDS             2392..3438
                     /gene="potD"
                     /locus_tag="ACHJZ2_003843"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO4569873.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHJZ2_003844"
     CDS             complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHJZ2_003844"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO4569874.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHJZ2_003845"
     CDS             complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHJZ2_003845"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO4569875.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCQGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHJZ2_003846"
     CDS             complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHJZ2_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO4569876.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHJZ2_003847"
     CDS             complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHJZ2_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO4569877.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHJZ2_003848"
     CDS             complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHJZ2_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO4569878.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHJZ2_003849"
     CDS             8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHJZ2_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO4569879.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            9971..13417
                     /gene="mfd"
                     /locus_tag="ACHJZ2_003850"
     CDS             9971..13417
                     /gene="mfd"
                     /locus_tag="ACHJZ2_003850"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO4569880.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHJZ2_003851"
     CDS             13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHJZ2_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO4569881.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14606..14863)
                     /gene="bhsA"
                     /locus_tag="ACHJZ2_003852"
                     /gene_synonym="ycfR"
     CDS             complement(14606..14863)
                     /gene="bhsA"
                     /locus_tag="ACHJZ2_003852"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO4569882.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         3726 a         3877 c         3809 g         3513 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccgtcgc cagttgtcgt tgatattctg caactggcgc
       61 gttccggagt gatagccatg agcgaacagt acctgataac gctcgacgag tggaaaccaa
      121 aacggttcag tctcccaata acaaacacta ccctggtgaa atacggaaaa ctaggataca
      181 tcgttccaag accacaaaaa attcgtgggc gttggctgat agatcgccga gcagtatttg
      241 ttgggcctgg tgaaacggga attgcgccgg aaattcatac tggcgatgat gatgcactga
      301 aggagatttt aactcatgtc accgaggcca cgaaaaaaca gcactgacgt agccggtctt
      361 tacgaaaagt ttgatcgcag aactggcaga gtttactacc agtataaaaa tcctgtgact
      421 ggaaaatttc acggactcgg aacagacaaa ggtaaggcag aaaaaatcgc ttccacagcc
      481 aatcagcgaa tagctgcagc agaagctgaa tatttcatgc gcaaaattga tgaaagtccg
      541 tcagcaacaa aacgtcgggg tatcagatta aaggcatggg ttgatcgata tctgaaaata
      601 caggacacgc gactgaaaaa tggagatatt gcagctacaa ctcacaaaga aaaaactcga
      661 atggctgcat acctggtttc ccgtctggga aaccacccat tgaaagaact ggaagtaaga
      721 gactttgcat taatactgga tgagtggctg gataaagaca tggtcagtac agcgagagta
      781 aatcgtggat tatgggttga tatttataaa gaagcacagc atgcagggga agttcctcct
      841 ggatggaatc ctccggaggc tacccgtaaa ccgatcccta aagtaaccag agccaggctc
      901 accatggaag actggcaaaa aatttacaat gcaacgcctg aaaaacactt tatccgtaac
      961 gcaatgcttc ttgcgattgt tactggtcag cgccgtgatg acatttgcca catgcgtttt
     1021 tcagatgtgt ggaacgaaca cttgcatatc acccagggaa aaaccggaat gcgtctggcg
     1081 ttaccgctta cactacgctg tgatgccatt gggataacgt taaaagaagt tattgatggg
     1141 tgccgagaca gaatattaag tccatatcta atccatagtc ggcaccagaa acaaccgaag
     1201 ccgatgagta aagacaacct gagcgactac tttgccaaag cacgggatct ggctggggta
     1261 attccaccag caggaaaaac tccgccaaca tttcatgaac aacgctctct atcagaacgg
     1321 ctgtaccgtg cacagggtat cgatacaaaa acattactag gacataaagt ccaggcaacc
     1381 accgatcgct ataacgatac tcgaggtcag gaatgggtta agttggttat ttgacaaaca
     1441 ccatatggaa tatccgtcac catctcagga aaaaagtgtt gtaaaatgcg tggcctagtt
     1501 ttggggaaac gttttggaga gattttggag aaaggaaaaa attgaataaa ttcaaaatcc
     1561 tgaaagtgtc ccgccttatg aataaaaagg tggaactcga atgatcggtc gactgcttcg
     1621 cggcggtttt atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt
     1681 gaactccttt aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta
     1741 tagcctgctg atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc
     1801 ggtgttttcg gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg
     1861 ttatcgcttt cgtggtaagc cgttcgttag cggaatgctg tttgtggtga tgatgtcacc
     1921 agatatcgtg atggcgattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg
     1981 cttctggtcg ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacggt
     2041 gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc
     2101 cagcgaattt accattctgc gaaaaatcat tctgccactg gcaatgccag cggtggcggc
     2161 aggctgggtg ttaagcttta ccctgtcgat ggacgatgtg gtggtttctt cgttcgtcac
     2221 cgggccgagt tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc
     2281 gccggaagtt aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc
     2341 cagccagctt attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa
     2401 tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc
     2461 gatgacaaca acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt
     2521 gaacagttca ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa
     2581 accatgtatg ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca
     2641 acctattacg tcgataaaat gcgtaaagaa gggatgatcc agaagatcga caagtcgaag
     2701 ttaacgaact tcagcaatct cgatccagac atgctcaata agccgtttga cccgaataac
     2761 gattactcca ttccgtatat ctggggtgcg acggcgattg gcgttaacgg tgatgcggtg
     2821 gatccgaaat ctgtcaccag ctgggccgat ttgtggaagc ctgagtacaa aggcagcctg
     2881 ctgctgaccg acgatgcccg tgaagtgttc cagatggcgc tacgtaagct gggctactcc
     2941 ggtaacacca ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg
     3001 ccaaacgtcg cagcgtttaa ctccgataac ccggctaacc cgtacatgga aggcgaagtt
     3061 aacctcggca tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgcctatt
     3121 gacgtggtgt ggccgaaaga aggcggcata ttctggatgg acagcctggc gatcccggca
     3181 aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg
     3241 gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg
     3301 ttaagtccag aagtggcgaa cgataagacg ctctccccgg atgctgaaac cattaaaaat
     3361 ggcgaatggc agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag
     3421 ctgaaagcag gacgttaatt cagcaagcgg gcggtggtat taccgcccgt tttttagtta
     3481 gcaaggatgt ggctaatgag tcaggacagt aaagttttat cccgctcttt cgtcggtatt
     3541 gtggtccgct tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat
     3601 cccttcagca acttttcaac aaattctggc accacctggc ttgccgggcc gtaatatttc
     3661 tcggcaaatt cattaccgac ctgacttggt tcaagattca gctccacggt gtgcgcgcca
     3721 tgcagtttcg cttcgtgaac aaacccagcc gccggataaa catgcccgga agtaccaatg
     3781 gcaatgaaaa tatcggccat cgacaacgcc atataaattt catccatgcc gagtggcatt
     3841 tcgccaaacc atactacgtg tgggcgcaag ggggccggga actggcagca atggcattta
     3901 tcttctgggg taacgtctcc ggtccagtcg agaacctgac cactttgtga acaacgaact
     3961 ttcagcagtt ccccatgcat atgaatcaca ttggtattac ctgcgcgttc atgcaggttg
     4021 tctatattct gcgtcaccag caaaaagcga tcgccgagag catcttgcag tttagccagc
     4081 gcaagatgcg cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta
     4141 taaaacgctt gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca
     4201 acccgatgtt cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca
     4261 gaaattcctg cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact
     4321 ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcggcgttt atttttacga
     4381 aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt
     4441 agatgtagga aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg
     4501 cgcggaacac gagctacagg taagagatga cgaggcagcc tgtccgccag ttgcgttgtg
     4561 attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc
     4621 aggatatttc cctgacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc
     4681 tgctcatcgc cttgatcata aagcgcaata atttcgggag ccggcaacgg ttgatgataa
     4741 tagtgttgat acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc
     4801 tgaccacagc cgcagcggcg taacgggaaa tccagcccca tcatggttaa cgcatcaacc
     4861 ggcagacgca tatggccaaa ctcgccggta atgtagcttt tcccggtaat cggtttgccg
     4921 ttgaaaatca gcccgccgcc aacgccggtg ccgagaatca accccatcac caacggatat
     4981 tgcgtaaatt cgtcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg
     5041 cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagca
     5101 gggacattgg cggcatacag cgtaccatct tctgtttccg gcattcccgg aataccgatg
     5161 ccgacagagc ctttacagcc aaaacgttga tcagcttcgg ctaccagctc gcacactgca
     5221 tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc
     5281 cgaccgctat caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac
     5341 attgccgctc cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc
     5401 aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt
     5461 gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata
     5521 gatatcgctg gagaggaact gatgaccgat cagcttttca atccgctcaa taatcggggt
     5581 aagttgcagt gaaacaacta cgccgataat cacaccacac aggctaccga atagccctgc
     5641 cagcaatcca taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct
     5701 taatactgcg atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt
     5761 gaaacaggcc acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg
     5821 gatatcgcga tacatatagc cgtaagtacc aatccagctt ttaatataaa catagctgtt
     5881 ggtcacttca cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag
     5941 ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat
     6001 catggcaaaa ctgtgatcga gttgaccact caactgcaaa ataccggcaa cgtgcaaacg
     6061 cacacgtttt ggctgcatca gtttatgctc aggattcgag ttggggatca taatcgacac
     6121 ccaatcgccc tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg
     6181 ttcgcccgct ttaaaattgc gccaggcatc cccctgaaca aacgagggta atgcgctcag
     6241 acgctgttcc tgttgcgggt taacgccctt cacctggatt gcgcgcagat tcgctccact
     6301 ttccaccagc ccggtgaaat tgatatacgg cgcagcggcg gcaatacctg gcactttttg
     6361 cacgttatcc agtgcttcct gccagttagt ccacggttga tccaccgcct ctatttcgcc
     6421 atgcggcacc accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct
     6481 taagccgacg atcaataccg ccacgccaag ggcaatgcca atggtagaaa tgacggagat
     6541 cagcgacacc atgccgccgc gtcgccgtcc gcggctaaaa cgcaggccaa tcaataacga
     6601 taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag acgaccatca
     6661 cgcatctcca gttggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg
     6721 aaggcggtgc cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtctgcgtta
     6781 cgcgcatcga ggttaccggt aggttcatcc gccagtacca gccacgggtt attgaccagc
     6841 gcacgggcaa tcgccacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc
     6901 gcacgatgct ccagccccac cgcttttaac atctcaagtg cacggctgtt gatttcagcg
     6961 ggctttttct tgccaatcag cagcggcata gccacgtttt ccagggcagt aaaatccggc
     7021 agcaggtggt gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc
     7081 gccgaagaca gtttgctcat cggttgaccg ttaaagatca catcgccgga ggttggtgta
     7141 tccagcccgc ccagcaggtg cagcaaggta cttttaccgg aaccagagct accgacgatc
     7201 gccatcattt caccttcgcc cacgctgaaa ctgacattgt gcagtacatc ggtttgcaca
     7261 ctgccttcct gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg
     7321 taaagcctca gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag
     7381 cgcgatagcc atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccaccgg
     7441 cagcgccgcg ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc
     7501 aagcagtgcg ccaagcgccg ctccgaggat cgcaccgata atcccggcgc tggccccttg
     7561 caccataaag accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgctac
     7621 ttcgccctgc ttctccatca ccatcagccc cagcgaggta ataatgttaa acgccgcaac
     7681 ggcgacaatc aggctcagca gtaagcccat catatttttt tccatgcgta cggcctggaa
     7741 cagctcgcct ttacgatcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg
     7801 acttaaagag tcaactttca gcggctcatc cagccacaaa cgccagccgg taatattgcc
     7861 tgccggataa cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc
     7921 gacttcactg ttagcggcaa aagtaccaat cacattgaac aggcgctgac ttggaatacg
     7981 ccccatcggc gtgaactggc tggcagatgg caccatcacg cggatttgat caccgcgatt
     8041 aacgcctagc tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag
     8101 gtcagtttgt ttcacattga ccagatacgg cgttagtgga tctttttgcg ccggatcgat
     8161 acccagcatc accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt
     8221 aataggtgcg acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg
     8281 gttaagagag ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt
     8341 ttgcagctcg cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc
     8401 gagggtaatg ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccgctgcacg
     8461 cccacgcatg taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg
     8521 gttgctgtag caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg
     8581 taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccatg
     8641 ttttatcatc aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata
     8701 cccattacac ctcgggtcat aacccgcagg atcaaccgca tgtaaattaa aaaagagcgt
     8761 gttgtcctga tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt
     8821 ctggtggtga tttatcactc ggtcattacc ttttatccgc atatgaccac ttttcagcat
     8881 ccgttatcgg aagtcctgag caaatgctgg atctatttca atctttacct tgcacccttt
     8941 cgtatgccgg tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta
     9001 ccgtggggaa attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg
     9061 ggcgtggtgc agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat
     9121 ttaagcaatg cctccaatgc cgcgtatgcc gattccaccg gtgagttcct gcacgggatg
     9181 atcaccgcca gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt
     9241 aaaattttta gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg
     9301 gttaatttcg ttcccacgcc gtggtgggga atgaacagtg tgatccgcaa tttgccttat
     9361 tacagccttg gtgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg
     9421 cgccgccatc tgctgatggc ttctttgctg gctgctctgg cggtcggtgc ctggttgttt
     9481 actatctcgc tgctgttgtc gctggtgtcg attgtggtga tcatgaagtt gttttatcag
     9541 tatgaacaac gttttggcat gcgttccacc agtctgctga atgtgattgg ctccaacact
     9601 attgctatct acactaccca tagaattctg gtagagatat tcagcttaac tctgcttgcg
     9661 caaatgaacg cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttacccc
     9721 tttgttagtt tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcga
     9781 gcattcagcg atctgttgtt ctccccgcct tcactgcccg ttgccgtcag ttactcccgc
     9841 taagccatcg ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg
     9901 aacagcattg cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct
     9961 gacaaccgtt atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg
    10021 tctgctgggc gagttaaccg gcgctgcctg cgcgacgctg gtagcggaaa ttgccgaacg
    10081 tcacgccgga ccggtggtgc ttattgcgcc agatatgcaa aatgccctgc gtttgcatga
    10141 tgaaattagc cagtttaccg accagatggt gatgaatctg gcggactggg aaaccctccc
    10201 ttacgacagt ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca
    10261 gctaccgaca atgcagcgtg gcgtactgat tgttccggtg aatacactta tgcagcgcgt
    10321 ttgcccgcac agttttctcc acggtcatgc gctggtgatg gaaaaaggtc agcgcctgtc
    10381 acgagatgca ttacgaaccc aactggatag cgccggttat cgccatgttg accaggtgat
    10441 ggagcacggc gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga
    10501 gttgccttat cgtcttgatt tctttgatga tgaaatcgac agtctgcggg tgtttgacgt
    10561 cgacagtcag cgcacgctgg aggaagtaga agcgatcaac ctgctgcctg cgcacgaatt
    10621 tccgaccgat aaagcggcga ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt
    10681 gaagcgcgat ccggaacata tttaccagca agtgagtaaa ggcacattac ctgccgggat
    10741 cgagtactgg cagccattgt tcttcagcga accactgccg ccgctgttca gttatttccc
    10801 tgccaatacc ttgctggtga atactggcga tctggaaaac agtgccgaac gtttccaggc
    10861 tgacacgctg gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccac tgttgccacc
    10921 acaatcgctc tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgagt
    10981 gcagctaaaa actgaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa
    11041 actgccagac ctggccgttc aggcacaaca aaaagcgccg ctggatgcgc tgcgtaagtt
    11101 cctcgagtct ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggcc gccgtgaagc
    11161 gctgggtgaa ctgctcgcgc gaattaaaat tgctccgcaa cgcattatgc gtcttgatga
    11221 agccagcgac cgtgggcgtt atctgatgat tggcgctgca gagcatggtt ttgtcgatac
    11281 ggtacgtaat ctggcgctga tttgcgaaag cgatctgctc ggtgaacgcg ttgcccgtcg
    11341 tcgtcaggat tctcgccgca ccatcaaccc cgatacactg atccgtaacc tcgcggaact
    11401 gcatattggt cagccggtgg tccatctgga gcacggcgtc gggcgctatg ccggaatgac
    11461 cacgctggaa gcaggcggca ttaccggcga gtatctgatg ctcacctatg ccaacgacgc
    11521 caaattgtat gttccggtgt cgtcgctgca tctgattagc cgttacgcgg gtggtgcgga
    11581 agaaaatgcc ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc
    11641 ggcggaaaaa gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc
    11701 caaagagggc ttcgcgttta aacacgatcg tgagcagtat cagttgttct gcgacagctt
    11761 cccgtttgaa accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg
    11821 tcagccgctg gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt
    11881 ggcgatgcgc gccgctttcc tggcagtaga taaccacaag caggtggcgg tgctggtgcc
    11941 taccaccctt ctcgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc
    12001 ggtacgtatc gaaatgctct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc
    12061 ggaagtggcg gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga
    12121 cgtcaagttt aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg
    12181 tcataaagag cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac
    12241 gccgatccca cgtacgctga atatggcaat gagcggaatg cgtgacctat cgattatcgc
    12301 cacgccgccc gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt
    12361 ggtccgcgag gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa
    12421 tgatgtggaa aacattcaga aagccgccga acggctggca gaactggtgc cagaagcgcg
    12481 gatcgccatc ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt
    12541 ccatcatcaa cgtttcaacg tgctggtttg tacaaccatt atcgaaaccg ggatcgacat
    12601 cccgacagcc aacaccatta tcattgaacg cgcggatcac ttcggtctgg cgcaactgca
    12661 ccagttacgc ggtcgcgtcg gacgttcgca tcatcaggca tatgcatggc tgctgacgcc
    12721 gcatccaaaa gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga
    12781 agatctcggg gcaggttttg cgctggcaac gcacgatctg gagattcgcg gcgcgggtga
    12841 actgcttggc gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga
    12901 gttgctggaa aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct
    12961 caccagccag caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat
    13021 tcctgacgtg aatacgcgtc tgtcgttcta taaacgtatt gccagcgcca aaacggaaaa
    13081 cgaactggaa gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cggatccggc
    13141 gcgtaccctg ctggatattg cccgtctacg ccagcaagcg cagaaactgg ggatcaggaa
    13201 gctggaaggt aatgagaaag gcggcgtgat cgaatttgcc gagaagaatc acgttaatcc
    13261 ggcctggttg attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac
    13321 gcgcctgaaa tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca
    13381 gtttatgcgt gaactggaag aaaacgcgat cgcttaatgg taaatcgggg gcgtttctgc
    13441 gcccccatct gcaacattta caaattcttt gcacttccct gcactatccg acactgtcac
    13501 catccataat tcaggcttat ctgtttatta caataacctt atatttatta tggatttttg
    13561 gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg
    13621 gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcggg cagtcgtctg
    13681 gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc
    13741 aaaaaatata acgtcggctt tctcgctcta ttacaggcta accccggcgt tgatccttac
    13801 gtaccgcgcg cgggcagcgt gttaacgatc ccgttgcaaa ccctacttcc agacgcgccg
    13861 cgcgaaggca ttgtgatcaa cattgcggag cttcgtctct attactaccc gccgggtaaa
    13921 aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg
    13981 acaatggtga ccaccgtttc tgacaagcgt gcaaacccaa cctggacgcc aacggcaaac
    14041 attcgcgcgc ggtataaggc acaaggaatt gagttgcctg cggtagtgcc ggctggaccg
    14101 gataatccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt
    14161 cacggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg
    14221 cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc
    14281 atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat
    14341 cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc
    14401 gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg
    14461 gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaacgctg
    14521 taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt
    14581 agcaggcatt aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat
    14641 tcggaccggt tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg
    14701 ccagctgctc ttccagcgat cccagatttg tccccgcgtt agcactgatt gtaccgactt
    14761 tttgttggcc ttctggcgtt gactgaactt cgacagccgc aaagctggca aatgacatgg
    14821 agcttaaaat cgccgcagcg atgagggttt ttacgttttt cataatagtg gccttatgca
    14881 gatgaatgac gtcgcaagca attacttaac gatcgataac taaat
//
LOCUS       DAZFBR010000113        14796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFBR010000113 DAZFBR010000000
VERSION     DAZFBR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.113"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            30..983
                     /locus_tag="ACHJZ2_003853"
     CDS             30..983
                     /locus_tag="ACHJZ2_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO4569883.1"
                     /translation="MDSNNQIESCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1192..2157
                     /gene="lipA"
                     /locus_tag="ACHJZ2_003854"
     CDS             1192..2157
                     /gene="lipA"
                     /locus_tag="ACHJZ2_003854"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO4569884.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2258..2461)
                     /gene="tatE"
                     /locus_tag="ACHJZ2_003855"
     CDS             complement(2258..2461)
                     /gene="tatE"
                     /locus_tag="ACHJZ2_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO4569885.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2590..3378)
                     /locus_tag="ACHJZ2_003856"
     CDS             complement(2590..3378)
                     /locus_tag="ACHJZ2_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO4569886.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3471..3854
                     /gene="crcB"
                     /locus_tag="ACHJZ2_003857"
     CDS             3471..3854
                     /gene="crcB"
                     /locus_tag="ACHJZ2_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO4569887.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3908..4117)
                     /gene="cspE"
                     /locus_tag="ACHJZ2_003858"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3908..4117)
                     /gene="cspE"
                     /locus_tag="ACHJZ2_003858"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO4569888.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4292..4852)
                     /gene="pagP"
                     /locus_tag="ACHJZ2_003859"
     CDS             complement(4292..4852)
                     /gene="pagP"
                     /locus_tag="ACHJZ2_003859"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO4569889.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            5441..6826
                     /gene="dcuC"
                     /locus_tag="ACHJZ2_003860"
     CDS             5441..6826
                     /gene="dcuC"
                     /locus_tag="ACHJZ2_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO4569890.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(6867..7547)
                     /gene="dpiA"
                     /locus_tag="ACHJZ2_003861"
                     /gene_synonym="citB"
     CDS             complement(6867..7547)
                     /gene="dpiA"
                     /locus_tag="ACHJZ2_003861"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO4569891.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(7516..9174)
                     /gene="dpiB"
                     /locus_tag="ACHJZ2_003862"
     CDS             complement(7516..9174)
                     /gene="dpiB"
                     /locus_tag="ACHJZ2_003862"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO4569892.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            9554..10612
                     /gene="citC"
                     /locus_tag="ACHJZ2_003863"
     CDS             9554..10612
                     /gene="citC"
                     /locus_tag="ACHJZ2_003863"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO4569893.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            10627..10923
                     /gene="citD"
                     /locus_tag="ACHJZ2_003864"
     CDS             10627..10923
                     /gene="citD"
                     /locus_tag="ACHJZ2_003864"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO4569894.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            10920..11828
                     /gene="citE"
                     /locus_tag="ACHJZ2_003865"
     CDS             10920..11828
                     /gene="citE"
                     /locus_tag="ACHJZ2_003865"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO4569895.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            11839..13371
                     /gene="citF"
                     /locus_tag="ACHJZ2_003866"
     CDS             11839..13371
                     /gene="citF"
                     /locus_tag="ACHJZ2_003866"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO4569896.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLSANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            13375..13926
                     /gene="citX"
                     /locus_tag="ACHJZ2_003867"
     CDS             13375..13926
                     /gene="citX"
                     /locus_tag="ACHJZ2_003867"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO4569897.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            13901..14779
                     /gene="citG"
                     /locus_tag="ACHJZ2_003868"
     CDS             13901..14779
                     /gene="citG"
                     /locus_tag="ACHJZ2_003868"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO4569898.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
BASE COUNT         3676 a         3880 c         3523 g         3717 t
ORIGIN      
        1 ttttccatga ataaaattgg agtaagtgcg tggatagtaa taatcaaatt gaatcctgtt
       61 taagcagaaa atcgtcagaa ggtaaaccac aaatatttac aaccttacga aatattgatc
      121 ttaatcttct gactattttt gaagctgtat atgtacataa agggatcgtt aatgcagcga
      181 aagtgcttaa tctgaccccc tcggcaatca gtcagtctat tcagaaactg cgcgttatat
      241 tccctgaccc attgtttatt cgcaaaggcc agggtgtcac tcctaccgca tttgcgatgc
      301 atctacatga gtatatcagt cagggccttg agtccattct tggcgcgctg gatatcgaag
      361 gaagctatga taagcaacga acgataacta ttgcgaccac cccctcggtc ggagctctgg
      421 tccttcctgt catttatcgg gcgattaaaa ctcactatcc gcagctttta ctgcgcaacc
      481 cgcccatcag cgacgcggaa aaccaactca gtcagtttca aaccgatctc atcatagata
      541 acatgttttg caccaatcgt acggtgcaac atcatgttct gttcaccgac aatatggtgt
      601 taatttgccg cgagggaaat ccactactct ctttagaaga tgacagagag actatcgaca
      661 acgctgcaca tgtactcctg ttaccggaag gacaaaattt cagcggtctg cggcagagaa
      721 ttcaagagat gtttccggac cggcagatca gcttcaccag ctacaacatt ttgacaatcg
      781 ctgcactggt tgccaacagt gacatgttag cgattattcc cagccgtttt tataacctgt
      841 ttagccgttg ctggccgctg gaaaaactgc cttttccgtc cttaaatgag gagcaaatag
      901 atttctctat ccactacaac aaattcagcc tgcgtgatcc catcctgcac ggagtaatag
      961 atgttatccg taatgcattt tgaaaaagta ttgttacagc cagatcacag cgcagaacga
     1021 taaaatggca caatccaaca acaattttac attttcgcga ccgctttggc tgctttcagg
     1081 tccgtttcaa tgatatactg ccagtcgtta attcaaaaat agttgataat tacaacaatc
     1141 tattgaattg aaacgctttc cttcgtaatt cgcaactgga acacgcacgc tatgagtaaa
     1201 cccattgtga tggaacgcgg tgttaaatac cgcgatgccg ataagatggc ccttatcccg
     1261 gttaaaaacg tggcaacaga gcgcgaagcc ctgctgcgca agccggaatg gatgaaaatc
     1321 aagcttccag cggactctac acgtatccag ggcatcaaag ccgcaatgcg caaaaatggc
     1381 ctgcattctg tctgcgagga agcctcctgc cctaacctgg cggaatgctt caaccacggc
     1441 acagcaacgt ttatgatcct gggcgctatt tgtacccgcc gttgtccgtt ctgtgacgtt
     1501 gcccacggtc gcccggtagc tcctgatgcc aatgagccag tgaaactggc gcagaccatt
     1561 gccgatatgg cgctgcgtta tgtggttatc acctccgttg accgtgatga cctgcgcgat
     1621 ggcggtgccc aacactttgc ggattgtatt actgccattc gggaaaaaag cccgcaaatc
     1681 aaaattgaaa ctctggtgcc ggatttccgc ggtcgtatgg atcgtgctct ggatattctg
     1741 actgcaacgc ccccagatgt gttcaaccat aacctggaaa acgtaccgcg tatttaccgg
     1801 caggtacggc ctggcgcaga ttacaactgg tcgctgaagc tgctggaacg ctttaaagaa
     1861 gcgcatccgg aaatcccgac caagtctggt ctgatggtgg gtctgggtga aaccaatgaa
     1921 gaaattattg aggtaatgcg cgacctgcgc cgtcatggtg tgacgatgtt aacgctgggg
     1981 caatatttgc agccaagccg ccatcacctg ccggttcaac gttacgttag cccggatgag
     2041 ttcgacgaaa tgaaagccga agcgctggcg atgggcttta cccatgctgc atgcggtccg
     2101 tttgtccgct cttcttacca cgccgatttg caggcgaaag ggatggaagt taagtaatcc
     2161 tgtaataatt acttacatct gtctgataaa aaacccgctt tttgaagcgg gttttttgta
     2221 tcgaacaaga tattgaggga gcgtcctgct cgccacgtca ctctttatga gagagctttt
     2281 cagcctgaag atcaacgtct gcgccttttt tcgccgcagc atcgtcatca ttcatcgcct
     2341 tcttgaaccc tttaatggcc gctccaaggt ctccgcccag cgtacgtaac ttcttagtcc
     2401 caaacagcag aacgaccagc gccgcaacta ccagcagttt ggtaatacta atctcaccca
     2461 tagatacctt cttgacatat aaccggcaac agaacgcgcc ttaaaaacca aagaatatta
     2521 acggtcattc gacgcgcgca cacaatagca atctgtaaca aggtgaatca agcgccgagt
     2581 aaaaaaacat cataataatt gcggcggcgc aaagcgacgg ttgtttaaga cgggcagttg
     2641 cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc ataatgagtg caggcatttc
     2701 tgacgctgcc gcaatggtga cgccaaaggg atctataatc cggctttgcc cgatattttt
     2761 gttcccgcac tcccccgccg ccaccatata acaggtggta tccagcgcac gagcggcaag
     2821 caacgttgac cagtggtgct ctttgagcgg cccgcgaacc caggcggcag gaagtaccag
     2881 gatttcagct ccctgtaatg cctgtgccag cgccagctct ggaaagcgta agtcataaca
     2941 ggtcatcaga ccgaccttca tcccctccac ctcgagtaac ggagcaattt cattaccagc
     3001 atcaacacgg cgtgattcct gaatggcaaa tgcatcatag agatgcaatt tggcataacg
     3061 ggcgacgatg tttcctgcct gtagcgccac cagcatattc catgcgcgcc ccggcgttga
     3121 aggaacatga atcgtcagaa ttgtcgtcat catgttacgt ttactttctc gccgtaaacg
     3181 tccgaggaat tcgccttcca gcagctgtgc tgatttaacc gatagatctg catcatgatc
     3241 atcgcgcgcc agcaatgctt ccggcaggac aaacagcgat acgtcgtttt ccgccgcctg
     3301 cgccatcaac gaggcacaaa tctcagcgtt cttttcccac acagatgtaa cagcaaactg
     3361 tcctgcagca accagcattg ccacctcctg tcagagtcgt aaaattcgcc aacgacgatt
     3421 attcggcgtt acacttatca ctcatacaaa tcaaatagca ggattttgca gtgttacaac
     3481 ttcttttagc agtttttatt ggcggtggta cgggaagcgt ggcgagatgg ctgttaagta
     3541 tgcgatttaa cccgctgcat caggcgattc cgttggggac gctggcagca aacctgattg
     3601 gggcattcat cataggaatg ggattcgcct ggttcagcag gatgacgaac attgatccag
     3661 tgtggaaagt attaatcacc accggatttt gtggcggtct aacaaccttc tcaacatttt
     3721 cggcagaagt ggtgtttttg ttacaagagg gccgctttgg ctgggcatta ctgaacgttt
     3781 tcgtcaacct tctggggtct tttgccatga ccgcactggc attctggctg ttttcggcct
     3841 caaccgcaca ctaaagacca aaaaaaaccc gctgattaag cgggttttga attcttgctg
     3901 acgtatctta cagagcgatt acgtttgcag cagaagggcc tttggcaccg ttagtgattt
     3961 cgaactctac gcgctgacct tcagcaagag ttttaaaacc attagtctgg attgcagaga
     4021 agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat gaaaccgaat cctttggact
     4081 cattaaacca cttaacgtta cctttaatct tagacatcaa aattaccttt acatgaaaaa
     4141 tagacacaaa tgctgtgtcg gttaccagta caccaattgt ggtacgcttt gtccagtgca
     4201 actttactaa aaagtgataa aaatcgcgtt atttttgatg ttacttattt tagttattga
     4261 ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg aaagcgcatc caggcaaagt
     4321 acacattgcc attgttgtag gtacccggaa tgtaggtcat ctgaaaagta actgggccat
     4381 aacccacgga ggccaatggc agtagaaccg ggagagggat gtaattccag ttatcgcgtg
     4441 ccgttacgcc agcggtgaat cccagaccta aatgaaaatt ttcatccgcc agcggtcgcc
     4501 aggtactttc ccatccgtat ccggcaatcg gttcccattt gttccacgag tccttaaatg
     4561 ccatggcata caggccatgc cagtttcctt tttcatccca acgcgacagg ccaaaaccgc
     4621 caccccacgg tcgctcgtta tagcgatcgg ttttttcttt gtcgtaagcg aaacgtgcat
     4681 gccaggtgat ggcaggaata tataaatcat aatgttcagg ctgttgccag gtttgtacaa
     4741 tattttctct aaacgttgtc atccactcat ctgcgttagc aaaaacttta ccaacgctga
     4801 ttaactgaat aaaaacaaag gaaaagatag cgacatattt actcacgttc atttgtgacc
     4861 ataaaacatt tatcaaaaat ctactactag catagcaaag ctacaattaa cataacctta
     4921 acagccccaa cataaagaat aatctgaata gctgctctat ttatttccgc ttgttcaagt
     4981 taaatatttc cgcgcatcga tttaaaatga cacatcgttt tctgcgatgg gaatagtcat
     5041 aaaagaaaaa gctagttgtt tctgacaaca aaatgcaacc aagaaaaaat gacttatttt
     5101 ttaataagat accaaaaatc atcttaacag ggaatatatt atatatcgtc aacccacgcc
     5161 cattgcagat tattttttga gcacacaacc gcagctaaca ccattgcaat taacaaattt
     5221 gcatcaatcc tccatcaatt tacacacatt attatgtgat aattgccaac cgctaaatat
     5281 gcgttttgtt atctacgtat aaaaacagca acttcaatgt cttaatggca gtttttcttg
     5341 attttaatca gcattcatcg ccaatttatt gggcatattt tttccttaag ctttaggaat
     5401 tttttattat ttactttggg gcctggagac aggaaaaatt atgctgacat tcattgaact
     5461 ccttattggg gttgtggtta ttgtgggtgt agctcgctac atcattaaag ggtattccgc
     5521 cactggtgtg ttatttgtcg gtggcctgtt attgctgatt atcagtgcca ttatggggca
     5581 caaagtgtta ccgtccagcc aggcttcaac aggctacagc gccacggata tcgttgaata
     5641 cgttaaaata ttactaatga gccgcggcgg cgacctcggc atgatgatta tgatgctgtg
     5701 tggatttgcc gcttacatga cccatatcgg cgcgaatgat atggtggtca agctggcgtc
     5761 aaaaccattg cagtatatta actcccctta cctgctgatg attgccgcct attttgtcgc
     5821 ctgtctaatg tctctggccg tctcttccgc aaccggtctg ggtgttttgc tgatggcaac
     5881 cctatttccg gtgatggtaa acgttggtat cagtcgtggc gcagctgctg ccatttgtgc
     5941 ctccccggcg gcgattattc tcgcaccgac ttcaggggat gtggtgctgg cggcgcaagc
     6001 ttccgaaatg tcgctgattg acttcgcctt caaaacgacg ctgcctatct caattgctgc
     6061 aattatcggc atggcgatcg cccacttctt ctggcaacgt tatctggata aaaaagagca
     6121 catctctcat gaaatgttag atgtcagtga aatcaccacc actgcccctg cgttttatgc
     6181 cattttgccg ttcacgccga tcatcggtgt actgattttt gacggtaaat ggggtccgca
     6241 attacacatc atcactattc tggtgatttg tatgctgatt gcctccattc tggagttcct
     6301 ccgcagcttt aatacccaga aagttttctc tggtctggaa gtggcttatc gcgggatggc
     6361 agatgcgttt gctaacgtgg tgatgctgct ggttgccgct ggggtattcg ctcaggggct
     6421 tagcaccatc ggctttattc aaagtctgat ttctatcgct acctcgtttg gttcggcgag
     6481 tatcatcctg atgctggtat tggtgattct gacaatgctg gcggcagtca cgaccggttc
     6541 aggcaatgcg ccgttttatg cgtttgttga gatgatcccg aaactggcgc actcttccgg
     6601 cattaacccg gcgtatttga ctatcccgat gctgcaggcg tcaaacctgg gccgtaccct
     6661 ttcgcccgtt tctggcgtag tcgttgcggt tgccgggatg gcgaagatct cgccgtttga
     6721 agtcgtaaaa cgcacctcgg taccggtgct tgttggtttg gtgattgtta tcgttgctac
     6781 agagctgatg gtgccaggaa cggcagcagc ggtcacaggc aagtaaatag taatgccgga
     6841 ggcaagtttc ctccggcttt atcatgtcac ccactgtggt atatgcgttg cggtctgcca
     6901 actttgccgt gaacaatttc agcaataatc agatggcggc tggcgcaata ttcaagataa
     6961 cgcctggcag tggtgcgact gatggtcagt gcctgcgcca ccgtttctgc cgtatgttgc
     7021 acaccaggct ctttaaacag ttttcgcacc gcgtttagcg tcaaggggtc aatgccggtc
     7081 ggcagttcgt ctttgggttc accgcgagca taggcattaa acatctcatc aatttgcttc
     7141 tggctggcgc tatcaatact ttccagcata tgtttacgct ggcggaaacg ggttagcgtt
     7201 tgccccagcc gttcataggc aatgggctta atgagataat caaatacacc acaacgtacg
     7261 gcttcagaca ccgtttccat atcgctggct gcagtggtaa acaccacgtc gccgggataa
     7321 tgcgcctgca ccagttcatg cagtaaatta atccctctac cgtcaggaag atagttatcg
     7381 agcaagatta gccccggctt aaaacgctcg atcatcattc gggcctgcgc caggtttccc
     7441 gccagtaata tctgactgaa tccgggaatg tgacgaatat attcagcatg catctctgcc
     7501 agcggcgttt cgtcctcaac gatcaatagg gttaatggag ctgtcatttg gtttcacttt
     7561 cggaatatag attgaaaata aggtaccgca gggatcatta tcttcgagag tgataacacc
     7621 accgcagcgc gttacgtagc tggcaatcaa gtacaaccca atgccatgtt caccgggctc
     7681 gtcagcacgc gtactgaccc cctgctcaaa tattttgtct cgtagagact ctggaacgcc
     7741 gcagccctga tcggcgactt caatcaccac atcatcgcct tcatcgctga ggaataattc
     7801 aacgatcttg tttccttcat cgctacgcag gctggcttcg aaggcgttat caagtaaatt
     7861 gcccacaatg gctgcaaact cggtgctatc cagtcctggc ggcagttgcg aaagctggct
     7921 accggggaca atgatcattt ttagccccag ttcccgggcg cgctgcactt taccaaaaag
     7981 cagccccgcc acctggcgat cggcaaacgc ctcgcgcagg ctgtcaataa gctgttgctg
     8041 ggcctgagac tccccctgca ccatcgccag cacgcgatca tactctttca tctgcaacag
     8101 accattgagc gtcgacatcc aattgagatg ctcgtgtcgc aatgtacgaa ggctctcaac
     8161 gtattgtttt atttgcgtca gttgcgcatt gagggtggaa atttcgtctt tactgcgaaa
     8221 gctgataatg gcccccagca aatcatcacc tgaacgaata gcttcccgat tggcaataac
     8281 gctcagaccg ttaaagttag ccaccacatc ctgacgtttt tcatcaatct gttcggtaaa
     8341 gaaatcggcg ggcctgacca cttcagcaat gggtttaccc aaccattgcc gtccgggaga
     8401 gctcagcccc agcatctttc ttgcgttacg attgatggcg gtaatgtaac catgcggatc
     8461 caccgcaatc agcccttcat aaaccgaact aaacagcgcc tcttgctgac ggaccacgcg
     8521 tgcgatttgc tttggctcca tgcccatcat ttgccgacgg atatgcgctg ccaggaacca
     8581 cgacagcaac atcagaatcc ctaacagcac gacaaacacg cctgccatcg gtaataaaaa
     8641 ctcagcccgc cagctatcga ttttactcac caggtagcca atcgacacca cgccgatgac
     8701 ttttccatcg tcatcaaaga ttggcgtttt ggcgcgcatc gccatcccca ttgacccttt
     8761 cccggtaatg aagtagctct cccctttctc cagcgcgccc tgtttggtga actgcatagg
     8821 ataaccaatt ttctccggat taggatggta aaggcggatc gagtgccggt ccccaatcac
     8881 cacataatca aaatcggtat ctctttgtaa tttgttagcg atggtcgcca gccgtttgta
     8941 gtcacgcgtt ttcacctcag agatgacact gtcattggag gcaataattt tcgcctgatt
     9001 cattgccatg tcgcgtacat gaagcgttaa atagtcctca aaactggccg taaaatattg
     9061 cgccagggct gcaatgacaa atattgagaa caccagaatc agcaaaaaga tacgcagcgg
     9121 aaatgccagt ctttggaaaa atgcaaactg tttattctcg ttaagctgca acattgtttt
     9181 ccttgcgggt tgtcccgagc ctctttgacg gatgtaatta aaatgttgaa tcaaaataca
     9241 tagatattaa ttatgatcgt ggcaaatttt attcattaaa tcaattaaat cacttaaacc
     9301 tgcgagtcat ttcgtaatta cattaattaa aaaacttaaa accattaaat aaataaaaaa
     9361 ccactaactc tatgtgaaat aaatcaaaat ttcacgccga aatactcctt aggatgtata
     9421 gcgaaaggag aaaaagatat acctcgatca cccccctttc tcccaagtga aaataaaagg
     9481 ttatcagttt gcaacattga acaacattcg ttgcaaatcg ataacaacat gcaccttcag
     9541 gatactattt attatgttcg gcaatgatat tttcacccgc gtaaaacgtt cagaaaataa
     9601 aaaaatggcg gaaatcgccc aattcctgca tgaaaatgat ttgagcgttg acaccacagt
     9661 cgaagtattt attaccgtaa cccgcgatga aaagcttatc gcgtgcggtg gaattgccgg
     9721 aaatattatt aaatgcgttg ctatcagtga atccgttcgc ggtgaaggac tggcgctgac
     9781 attagccact gaactgataa acctcgccta tgagcggcac agcacgcatc tgtttattta
     9841 taccaaaacc gaatacgagg cgctgttccg ccagtgcggt ttttccacgc tgaccagcgt
     9901 acccggcgtg atggtgctga tggaaaacag cgccacgcga ctgaaacgct atgccgaatc
     9961 gctgaaaaaa tttcgtcatc cagggaacaa gattggctgc attgcgatga acgccaatcc
    10021 ctttacgaat ggtcaccgtt atctgattca acaagctgcg gcacagtgcg actggttgca
    10081 tctgttttta gtcaaagaag attcttcacg cttcccctat gaagaccggc tggatctggt
    10141 gttaaaaggc accgccgata ttccacgcct gactgtgcat cgcggctccg aatacatcat
    10201 ctcccgcgct acgttccctt gctacttcat taaagaacag agcgtcatta accattgtta
    10261 caccgaaatt gatctgaaga ttttccgtca gtacctcgct cccgcgctgg gtgtaactca
    10321 ccgctttgtc ggtactgaac ccttttgtcg cgttaccgcc cagtacaacc aggatatgcg
    10381 ctactggctg gaaacgccga ctatctccgc agcgcccatc gaactggttg aaattgagcg
    10441 gctgcgttac caggagatgc cgatatccgc ttcccgggta cgtcaactgc tggcgaaaaa
    10501 cgatctcacg gctatcgcgc cgctggtccc tgcagtcacg ctgcattatt tgcagaacct
    10561 gcttgagcac tcccgccagg acgcggcagc tcgtcaaaag acccccgcat gagaaacagg
    10621 tgaaaaatga aaataaacca gcccgccgtt gcaggcaccc ttgagtctgg ggatgtgatg
    10681 atacgcatcg ccccactcga tacgcaggat atcgacctgc aaatcaatag cagcgttgag
    10741 aaacagtttg gcgatgcaat tcgcaccacc attctggacg ttctcgcccg ctacaacgtg
    10801 cgcggcgtac agctgaatgt cgatgataaa ggcgcactgg actgcatttt acgtgcacga
    10861 ctggaagccc tgctggcacg cgccagcggt atcccggctc tgccatggga ggattgccaa
    10921 tgatttccgc ttcgctgcaa caacgtaaaa ctcgcacccg ccgcagcatg ttgtttgtgc
    10981 ctggtgccaa tgccgcgatg gtcagcaact ccttcatcta cccggctgat gccctgatgt
    11041 ttgacctcga agactccgtg gcattacgtg aaaaagacac cgcccgccgc atggtttatc
    11101 acgcgctgca acatccgctg tatcgcgata ttgaaaccat tgtgcgtgta aacgcgctgg
    11161 attccgaatg gggtgttaac gacctggaag ccgtcgttcg cggtggtgcg gacgttgtac
    11221 gtctgccgaa aaccgatacc gctcaggatg ttctggatat cgaaaaagag atcctgcgta
    11281 tagaaaaagc ctgtggtcgt gaacccggca gcaccggcct gctggcggcg attgaatctc
    11341 cgctgggcat tacccgcgca gtggaaatcg ctcacgcttc cgagcgtttg atcggtatcg
    11401 ccctcggtgc agaagactat gtgcgcaacc tgcgtacaga acgctccccg gaaggaactg
    11461 aactgctgtt cgcacgctgt tccattttgc aggccgcgcg ctctgcgggt attcaggcgt
    11521 tcgataccgt ctattccgac gctaacaacg aagccggatt cctgcaagaa gccgcccaca
    11581 tcaaacagct gggctttgac ggcaaatcgc tgatcaaccc gcgtcagatt gatctgctgc
    11641 acaacctcta cgcaccgacc cagaaagaag tggatcacgc ccgccgcgtc gtagaagccg
    11701 ctgaagccgc cgctcgcgaa ggcctcggcg tggtttccct gaacggcaag atggtagacg
    11761 gtccggttat cgatcgtgcc cgtctggtgc tctcccgtgc agaactttcc ggcatccgcg
    11821 aagaataagg caatcaaaat gacgcagaaa attgaacaat ctcaacgaca agaacgggta
    11881 gcggcctgga atcgtcgcgc tgaatgcgat cttgccgctt tccagaactc accaaagcaa
    11941 acctaccagg ctgaaaaagc gcgcgatcgc aaactgtccg ccaacctgga agaagcgatt
    12001 cgtcgctctg gtttacagga cggcatgacg gtttccttcc atcacgcttt ccgtggcggt
    12061 gacctgaccg tcaatatggt gatggacgtc atcgcgaaga tgggctttaa aaacctgatc
    12121 ctggcgtcca gctccctgag tgattgccat gcgccgctgg tagaacacat tcgccagggc
    12181 gtggttaccc gcatttatac ctccggcctg cgtggtccac tggcggaaga gatctcccgt
    12241 ggtctgctgg cggaaccggt gcaaatccac tctcacggcg gacgtgtgca tctggtacag
    12301 agcggcgaac tgaatatcga cgtggctttc ctcggcgtcc cgtcctgtga tgaattcggt
    12361 aatgccaacg gctacaccgg taaagcctgc tgcggctccc tcggctatgc aatggttgat
    12421 gccgacaacg caaaacaggt tgtgatgctt accgaagaac tgctgcctta tccgcataat
    12481 ccggcaagca ttgagcaaga tcaggttgat ttaatcgtca aagttgatcg cgttggcgat
    12541 gctgcaaaaa tcggcgctgg tgctacccgt atgaccacta acccgcgcga actgcttatt
    12601 gcccgtagcg ctgcggatgt gattgtcaac tcaggctact tcaaagaagg tttctccatg
    12661 caaaccggca ccggcggcgc atcgctggcg gtaacccttt tcctggaaga caaaatgcgt
    12721 agccgcgata ttcgcgccga cttcgccctc ggtggtatta ccgcgacgat ggttgatctg
    12781 cacgaaaaag gtctgatccg caaactgctg gatgtacaga gctttgacag ccacgctgcg
    12841 caatcgctgg cccgtaaccc caatcacatc gaaatcagcg ccaaccagta cgctaactgg
    12901 ggttcgaaag gtgcatcggt tgatcgtctc gacgtggtgg tactgagcgc gctggaaatt
    12961 gacacccagt tcaacgttaa cgtgctgacc ggctctgacg gcgtactgcg tggtgcttcc
    13021 ggtggtcact gcgataccgc gattgcctct gcgctttcca tcatcgtcgc gccgctggta
    13081 cgcggtcgta ttccgactct ggtggataac gtgctgacct gcatcacccc gggctccagt
    13141 gtcgatattc tggtcacaga ccacggtatc gcagttaacc cggcacgtcc ggaactggca
    13201 gaacgtctgc aggaagcggg cattaaagtg gtttccattg agtggctgcg cgaacgtgcg
    13261 cgtctgctga ccggtgaacc acagccgatt gaattcaccg accgcgtcgt tgccgttgtg
    13321 cgttaccgcg atggctcggt gatcgatgtt gtgcatcagg tgaaggaata agccatgcac
    13381 ctgcttcctg aactcgccag ccaccatgcg gtgtcaattc ccgagctgct cgtcagccgg
    13441 gatgaaaggc aagcacggca acacgtctgg ctcaagcgcc atcctgttcc actggtctcc
    13501 tttaccgtgg ttgcgcctgg gccgattaaa gacagtgagg tcacacgccg aatttttaat
    13561 catggcgtga cagccttgcg tgccttagcc gcaaaacagg gctggcaaat tcaggagcag
    13621 gctgcactgg tttccgccag cgggccggag ggtatgttga gcattgccgc cccggctcgc
    13681 gacctcaagc tcgccaccat tgagcttgaa catagtcatc ctctcgggcg gttatgggat
    13741 atcgatgtcc tgacgcccga aggcgaaatt ctctcccgcc gcgactattc actgccgcct
    13801 cgccgctgcc tgttgtgcga gcaaagcgca gccgtctgcg cgcgtggaaa aacccatcaa
    13861 ctgaccgatt tactcaaccg catggaggca ctgctgaacg atgtcgatgc ctgcaacgtt
    13921 aactaaaacc acaaagcttg cgacgtcatt aatcgatgag tacgccctgc tgggctggcg
    13981 cgccatgctg actgaagtca atctgtcacc gaaaccaggt ctcgtggatc gcattaactg
    14041 cggtgcgcat aaagatatgg cgctggaaga tttccaccgc agcgcgctgg cgattcaggg
    14101 ctggctaccc cgtttcattg aatttggtgc ctgtagtgcg gaaatggcac cagaagcggt
    14161 actccacgga ttacgcccaa ttggtatggc ttgcgaaggt gatatgttcc gcgccaccgc
    14221 gggcgtaaac acgcataagg gcagcatttt ttctttaggg ctactgtgtg cggcaattgg
    14281 ccgtttgctt cagctcaacc agttgctaac gccaacaacc gtttgttcta cggcggcaag
    14341 tttctgccgt ggcctgaccg atcgcgaact gcgtaccaat aattcacaac tgacggcagg
    14401 tcaacggttg taccagcagc ttggccttac cggcgcacgc ggtgaagccg aagcgggtta
    14461 tccactggtg atcaaccacg ccttgccgca ttacctcact ctgctggatc aggggttaga
    14521 tcctgaactg gcattgctcg ataccttgct cctactgatg gcgatcaacg gcgataccaa
    14581 cgttgcttcg cgcggtggcg aggggggcct gcgctggcta cagcgcgagg cgcaaacatt
    14641 attgcaaaaa gggggcattc gaacccccgc agatctcgat tatctccggc agttcgacag
    14701 ggagtgtatc gaacgaaatc tcagtccagg cggcagtgcc gacctgctga tccttacctg
    14761 gtttttagca cagatttaat tatttaagaa cttgat
//
LOCUS       DAZFBR010000114        14771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFBR010000114 DAZFBR010000000
VERSION     DAZFBR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.114"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..816
                     /locus_tag="ACHJZ2_003869"
     CDS             <1..816
                     /locus_tag="ACHJZ2_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4569899.1"
                     /translation="LNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHN
                     RMGQIFRKVLIDVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAE
                     KDGYFLQRGMLLALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKL
                     NLTLDDVVSSCRDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRA
                     NGTPPSFHEQRSLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(851..1489)
                     /gene="ompW"
                     /locus_tag="ACHJZ2_003870"
     CDS             complement(851..1489)
                     /gene="ompW"
                     /locus_tag="ACHJZ2_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO4569900.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1729..1818
                     /locus_tag="ACHJZ2_003871"
                     /pseudo
     CDS             1729..1818
                     /locus_tag="ACHJZ2_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1846..2589
                     /gene="yciC"
                     /locus_tag="ACHJZ2_003872"
     CDS             1846..2589
                     /gene="yciC"
                     /locus_tag="ACHJZ2_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO4569901.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2619..3158
                     /gene="yciB"
                     /locus_tag="ACHJZ2_003873"
     CDS             2619..3158
                     /gene="yciB"
                     /locus_tag="ACHJZ2_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO4569902.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3263..3661
                     /gene="yciA"
                     /locus_tag="ACHJZ2_003874"
     CDS             3263..3661
                     /gene="yciA"
                     /locus_tag="ACHJZ2_003874"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO4569903.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(3701..4420)
                     /gene="tonB"
                     /locus_tag="ACHJZ2_003875"
     CDS             complement(3701..4420)
                     /gene="tonB"
                     /locus_tag="ACHJZ2_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO4569904.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4644..4940
                     /gene="yciI"
                     /locus_tag="ACHJZ2_003876"
     CDS             4644..4940
                     /gene="yciI"
                     /locus_tag="ACHJZ2_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO4569905.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5241..6494
                     /gene="kch"
                     /locus_tag="ACHJZ2_003877"
     CDS             5241..6494
                     /gene="kch"
                     /locus_tag="ACHJZ2_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO4569906.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(6549..6722)
                     /gene="yciY"
                     /locus_tag="ACHJZ2_003878"
     CDS             complement(6549..6722)
                     /gene="yciY"
                     /locus_tag="ACHJZ2_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO4569907.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            6865..8325
                     /gene="cls"
                     /locus_tag="ACHJZ2_003879"
     CDS             6865..8325
                     /gene="cls"
                     /locus_tag="ACHJZ2_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO4569908.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8360..8689
                     /gene="yciU"
                     /locus_tag="ACHJZ2_003880"
     CDS             8360..8689
                     /gene="yciU"
                     /locus_tag="ACHJZ2_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO4569909.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(8742..9746)
                     /gene="oppF"
                     /locus_tag="ACHJZ2_003881"
     CDS             complement(8742..9746)
                     /gene="oppF"
                     /locus_tag="ACHJZ2_003881"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO4569910.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(9743..10756)
                     /gene="oppD"
                     /locus_tag="ACHJZ2_003882"
     CDS             complement(9743..10756)
                     /gene="oppD"
                     /locus_tag="ACHJZ2_003882"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO4569911.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(10768..11676)
                     /gene="oppC"
                     /locus_tag="ACHJZ2_003883"
     CDS             complement(10768..11676)
                     /gene="oppC"
                     /locus_tag="ACHJZ2_003883"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO4569912.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(11691..12611)
                     /gene="oppB"
                     /locus_tag="ACHJZ2_003884"
     CDS             complement(11691..12611)
                     /gene="oppB"
                     /locus_tag="ACHJZ2_003884"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO4569913.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(12697..14328)
                     /gene="oppA"
                     /locus_tag="ACHJZ2_003885"
     CDS             complement(12697..14328)
                     /gene="oppA"
                     /locus_tag="ACHJZ2_003885"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO4569914.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         3717 a         3621 c         3497 g         3936 t
ORIGIN      
        1 ctcaacacgc tgaaacagaa agcggcccct ctcagggtat ttgatgaacg ttttggcacc
       61 agaccgttag atgccataac cgtaaaagat gtggtatcag tactggaaga gtacaaggcc
      121 agaggacata acagaatggg acaaattttc aggaaagtac tgatcgatgt tttccgggaa
      181 gctcagcaaa cgggcgatgt cccgccaggc tttaaccctg cagaatcggc aaaaaaaccg
      241 caggtgcgga tatcaagaca gcgactgact tttgatgagt ggatgatgat ttataacgca
      301 gcggaaaagg atggttactt tttacagcgc ggtatgctgc tggcactgat gacaggccag
      361 cgcctttcag atatttgcaa aatgcaattt tcggatatcc gggatggtta tcttcatgtc
      421 gaacagcaaa aaacaggaac ccggattgcc atccctctgg ctctgcgttg cgataaatta
      481 aatctcaccc tggatgatgt ggtgtcatcc tgccgcgatt gcgttcttag tccgtggcta
      541 ttgcaccacc atcacgcgaa agggacagct aagcgcggcg ggatggttaa gccagcaaca
      601 ttaaccgttg catttaaaaa agcccgggat tctgtggatt acaactggcg tgctaatggc
      661 acccctccct ctttccatga gcagagatct ttatcagagc gattgttcag agagcagggg
      721 gttgatacca aaattttgct aggccattcg aatcaaaaaa tgaccgatat ttacaacgac
      781 gcacgcggta aggaatggaa aaaactggtc atttgatgac cagttttgca gaggggtttt
      841 gcagagaaaa ttaaaaacga tatcctgcgg agaacataaa cacccacgga tccagtcgta
      901 ccgagtcttt tacggtagta acaccagatt tgtacttagc cgtggtatcg atatccatgt
      961 accacactga catgttaacc aaccagtcac ggttaatcag ataatcaacc cccacctgcc
     1021 cggcagctcc ccaggaatct ttcagactga gatcggaaag ccccgcctct ttgccatgat
     1081 cgttaaatcc attatcaaag aaggtggtgt agttaatacc tgccccaaca taaggacgga
     1141 atttgctgct ggcatcacca aaataccact gcgccatcag tgttggcggc agatgatgaa
     1201 cggttgcaat atcgccggtc gcccgggtgc cgattttatg gcggaacggc gtcgctgcca
     1261 gtaattccac accaatgttg tcggtcgcca tataagtaaa cgtcaggccc agttgagtgt
     1321 tattggtcac gctgaatcca cccagacttc ctaacgtacc accagcacct tctgttggac
     1381 gtacggttgc agaacctgca cgcataaaaa attcgcctgc ttcatgcgca aaggcactgc
     1441 cagagagaag agttgttact gccaaagccg ccactgttaa ctttttcata tccgctccgt
     1501 cgttatggtt ataaaaacgt gacgaatata cctacattgg agtaacaaat gatcctacat
     1561 agatcacatt acatatgata attcaccatt tattgatctg gattaattta gaagttgcat
     1621 tgagaatcct ggttaacgtg atttaaatca atttttaaat attttcgcta cgcaaatatt
     1681 aactcgttcg ccacttgccg cggtttgccc acgccttaag gccagcaatt acctccgctt
     1741 tacaaagata tgcttttcca tacaagacct gatgctgcca cccgaggctt aaccagggta
     1801 caattgcccg ctaattaaca cctgcataaa actcaaggag agtgcatgtc tatcacggcg
     1861 cagtccgtat accgtgacac cggaaatttc ttccgtaatc aatttatgac cattctgttg
     1921 gtatcgttgc tatgtgcgtt tatcacagtg gtgttagggc atgttttctc acccagtgat
     1981 gcacagcttg cgcagctcaa tgacggcgtg cccgttagcg gcagtagtgg gttgttcgac
     2041 ctggttcaga atatgtcacc ggaacagcaa caaattttgc tgcaggcttc agcggcgtcc
     2101 actttttcag gattaatcgg taacgccatt ctcgccggag gcgtaatatt aattatccag
     2161 ctggtgtctg cgggtcagag agtcagtgcg ctgcgagcta ttggtgccag tgcgccgata
     2221 ttgccaaagt tatttattct gatttttctg actacccttt tagtacagat tggcatcatg
     2281 ctggtggtcg ttccgggaat tatcatggcc atattactgg ccctggcacc ggtgatgctg
     2341 gtacaggaca aaatgggcgt ttttgcctcg atccgtagca gtatgcggct gacttgggcg
     2401 aatatgcgtc tggtggcacc cgcagtactg agctggttgc tggcaaaaac actgttgctg
     2461 ctttttgcct cttcttttgc cgcattaacc ccggaaattg gtgccgtact ggcgaacacc
     2521 ttgagcaacc tgatttcagc catattgctc atctatctgt tccgcctgta tatgttgatt
     2581 cgccaataac cttaagcatt tgattacgga atcgtaaaat gaagcagttt cttgattttt
     2641 taccgctggt tgtctttttc gcgttttaca aaatttatga catctatgcg gctactgcgg
     2701 cgctgatcgt cgccacggcg attgtgctta tctatagctg ggttcgcttt cgtaaggttg
     2761 agaagatggc cctgatcact tttgttctgg tggtcgtctt cggtggcttg acgctgttct
     2821 tccacaatga tgagtttatt aaatggaagg ttacagtcat ttatgctctg tttgcgggag
     2881 ccctgttagt cagccaatgg gtgatgaaaa agccgctaat tcagcggatg ctgggtaaag
     2941 aactcacgct gccgcaaccg gtatggtcga agctgaatct ggcctgggct gttttcttta
     3001 tcctttgcgg tctggcaaac atctacatcg cattctggct gccgcaaaat atttgggtca
     3061 actttaaagt ctttggcctg accgccctta ccttaatctt cacattgtta agcggtatct
     3121 atatctaccg ccacatgccg caggaagata aatcctaacc agactgccag tcctttgggg
     3181 ctggcgctgt atctcctctg ttctcctcag gaaaatcata gtagcatcgc gcctgtgatt
     3241 ttccttttaa gtcggtttta ccatgtctac aacacataac gtccctcagg gcgatcttgt
     3301 tttacgtact ttagccatgc ccgccgatac caatgccaat ggtgacatct ttggtggttg
     3361 gttaatgtca caaatggata ttggcggcgc tattctggca aaagaaattg cccacggtcg
     3421 cgtagtgact gtgcgggttg aaggaatgac tttcttacgg ccggttgcgg tcggcgatgt
     3481 ggtgtgctgc tatgcacgct gtgtccagaa agggacgaca tcggtcagca ttaatattga
     3541 agtgtgggtg aaaaaagtag cgtctgaacc aatcgggcaa cgctataaag cgacagacgc
     3601 attatttaag tatgtcgcgg ttgatcctga aggaaaacct cgcgccttac ctgttgagta
     3661 atagtcaaaa gcctccggtc ggaggctttt gactttctgc ttactgaatt tcggtggtgc
     3721 cgttaatttt aaacaggata ttcaccacaa tcccactgcc tggcttaccc ggctcataac
     3781 gccatctgcg catcgcattt ttcacctcac gctcaaacat gttcgcaggc ttggctgaga
     3841 ggatttgtac gttatccacg cgaccatccg gcgtgacgtc aaatttaact ttaacctgcc
     3901 cttcaatgcg caatgcctgt gctcgtgccg gatactgcgg ctgattacgg cttaatgcgc
     3961 gtggtcctga agccacactg gtaaccggct tgctggttgc agccgttgct gtacttgatg
     4021 tcgggcgtgc cggtgccgta ttttcaaacg gtgatgccgg acgcgactct acgggtttga
     4081 catcgcgttt ttgctgctcc tgtacctttt tcaccggctt cggttttggc ttaggtttcg
     4141 gcttcggctt ttcaatgacc accggtgctt cttttggcgg ttcggggatc ggctcaggtt
     4201 ccggttctgg ctctaccacc ggctccggtg gcggctgaac ggcttgtggc ggttcgagat
     4261 cagcaggcgc aaccatcgtg acagaaatcg gctgcgcagg cgcaggtagt tcaataacct
     4321 gatgtaccga ggtatagagc agacccgcca caacagcacc atgaatgcag accgaaagta
     4381 acgtcggcca ggggaagcgg cgaggtaaat caagggtcat tgaagtcata atcatttcag
     4441 tagaaaaacc aggtctcgat tttaaatgca aatagcaatc atattcaata aggcaagacg
     4501 atcaggatca gtgaaaatgt catttttggc gtaattgacc ttactttatg catggtctta
     4561 acaaagacat catctttccg ttgcagatct tcgccctttc aaataacgta ctttacaact
     4621 ttcccgaaca aggagttgtg cccgtgttgt atgttattta cgctcaagac aaagctgata
     4681 gcctcgaaaa acgcctttcc gttcgtccgg cacatttagc acgtttacag ttactgcatg
     4741 atgaaggtcg tttgttgaca gctggtccaa tgccagcggt agacagtaac gatccagggg
     4801 ctgcgggatt tactggctca accgttattg ctgaatttga atcgctggaa gcagctcagg
     4861 cctgggccga tgctgacccg tacgtagcgg caggagtcta tgaacacgtt tcagtgaaac
     4921 catttaagaa agtgttctga gtagagatcc aatatttaag ggcacggttt ttgcaaccgt
     4981 gttcttgttt ggcctttcct tataggtcgg tcattgcatt caccaccaca agcagataat
     5041 aactttatct gtatttcttt atatgatgtg agcatgttca ggaaaaatgc cctttcctga
     5101 cgcagagttg ctcactcaaa acctttgatt actgcggata aagtcatcta ctattgttta
     5161 acacattcac gtgtcgcatg tatgttcaga aaatgtgcac gttaagataa ttaaacaaaa
     5221 ttgtggttac agaggtatca gtgagtcact gggctacatt caaacaaaca gcaacaaatt
     5281 tatgggtgac cctacggcac gacattctcg cgctggccgt ctttttaaat gggttgctta
     5341 tttttaaaac aatctatggt atgtcggtca atttgcttga tattttccat atcaaagcat
     5401 tttcagagtt ggatctctcc ttgctggcaa acgccccact atttatgctc ggcgtctttc
     5461 ttgtcctgaa ctccattggc ttactgttcc gggcaaagct cgcatgggca atcagtatca
     5521 ttttgttgtt gatagcgcta atttacaccc tgcattttta tccctggctg aaatttagta
     5581 ttggattttg catttttacg ctggtgtttt tactgatact gcgcaaagac ttctcccaca
     5641 gtagcgccgc agccgggaca atttttgcat ttattagttt cacgacatta ctgttttact
     5701 caacctacgg tgcgctttat ttaagcgaag gttttaatcc gcgaatagaa agtttgatga
     5761 ccgcgttcta tttttcgata gaaaccatgt caaccgtcgg ctatggcgat attgtccctg
     5821 tttctgaatc agcacgattg ttcactattt cggtcattat ttccggcatt accgtttttg
     5881 ccacatctat gacctcaatt tttggtccgc ttatccgcgg gggattcaac aaacttgtaa
     5941 aaggaaacaa tcatacaatg catcgtaaag atcattttat tgtttgcgga cattcgattc
     6001 tcgccattaa tacgatcctg caactgaatc aacgcggaca aaacgtaacg gttatcagca
     6061 acttgcctga agatgatatc aagcaacttg agcaacgctt aggcgataac gctgatgtta
     6121 tccccggtga cagtaatgac agttctgtat taaagaaagc gggaatcgat cgttgccggg
     6181 ccattctggc gctgagtgat aacgatgcag ataacgcgtt tgttgtactc tcggcaaaag
     6241 atatgagcag tgatgtcaaa acagttctcg ccgtcagtga tagcaaaaac ctgaataaga
     6301 ttaagatggt acatccggat atcattctct caccgcaact gtttggcagc gaaattctgg
     6361 cgcgagtttt aaatggtgaa gagattaata atgatatgct cgtttcaatg ttgttgaact
     6421 caggtcatgg tattttcagc gataacgatg aacaagaaac gaaagctgac agtaaagaat
     6481 cggcgcaaaa atagagttta aagacggaag agtaaactta ttgggcaccg caatggtgcc
     6541 cagtacattt agatattgta gatcgcaaac aataacgagt tacgctgttt gtttagaatg
     6601 cacttcctga tgctgtggat acgcatattt cggcgcgact gcccctcaag ccaacgcgat
     6661 ttacgtcggc tagcctgacg ctgcatgcgc cagcgcccca cttccgttct actccgcttc
     6721 atgttaacta ctctatgcaa taacagaatg gtcattatac ccccgccccc gacgctgacc
     6781 agtgcttttc ccgcgttttt atttgccaga tgaatccata tgcgtaaact cataacaatg
     6841 cgctttcaaa aggatttcta atctatgaca accgtttata cgttggtgag ttggttggcc
     6901 attctgggat actggttgct cattgcaggc gtaactttac gcattctaat gaaacgacgc
     6961 gcagttccct ccgcgatggc ctggctgttg attatttaca ttctgccgtt agtcggaatt
     7021 attgcctatc ttgccgttgg cgagctccat ttaggcaaac gccgcgctga gcgcgccaga
     7081 gcgatgtggc cttccaccgc aaaatggctt aacgacctta aagcctgtaa gcatatcttt
     7141 gccgaagaaa atagcagtgt cgctgcgcca ttattcaagc tttgcgagcg tcgtcagggg
     7201 atcgctgggg tcaaagggaa tcagctacaa ctgatgaccg agtcagatga tgtgatgcag
     7261 gcgttaatcc gcgacatcca gctcgcgcgc cataatattg agatggtgtt ttatatctgg
     7321 cagcccggcg gcatggcgga ccaggtggct gaatcattaa tggcggctgc gcgacgcggc
     7381 attcattgcc gattgatgct cgactccgcc gggagtgtag cttttttcca cagcccgtgg
     7441 cccgagctaa tgcgtaatgc cggtattgaa gtggtcgaag ccttaaaggt caatctgatg
     7501 cgtgtgtttt tacgccgtat ggacctgcgc caacatcgca agatgatcat gatcgataat
     7561 tacatcgcgt acaccggcag catgaatatg gtcgaccctc gctacttcaa acaagatgcg
     7621 ggcgtagggc aatggattga tctgatggcg cgtatggaag gccccatcgc caccgcgatg
     7681 gggattattt attcctgcga ctgggagatt gaaacaggga aacgtattct accgccacca
     7741 cctgatgtca atattatgcc gtttgagcag gccagcggtc ataccattca cacaattgct
     7801 tctggccccg gctttccgga agatctcatt caccaggcat tattgactgc ggcttattcg
     7861 gcgcgtgaat atttgatcat gaccacgccc tactttgtgc caagcgatga tttacttcat
     7921 gcgatttgca cggcggcgca gcgcggggtg gatgtcagta ttatccttcc gcgaaaaaat
     7981 gactcgatgc tggtcggctg ggccagtcgc gcattcttta cggaactgct tgctgctggg
     8041 gttaaagttt atcagtttga aggcgggtta ctgcatacca agagcgtgct ggtcgatggc
     8101 gaactaagtc tggttggcac cgttaacctt gatatgcgta gtctgtggct gaatttcgag
     8161 attaccctgg caatcgacga taaaggtttt ggtgcagacc ttgccgccgt tcaggacgat
     8221 tatatttcgc gttcacgtct gctcgatgcc cgtttatggc taaaacgtcc attatggcaa
     8281 cgtgtcgccg agcgactgtt ttacttcttc agcccgttgc tgtaaaacgt cgccaacaga
     8341 ggttaaacag gtagtgacta tggatatgga tctaaacaat cgcctgactg aagatgaaac
     8401 gcttgagcag gcctacgata tttttctcga actggctgcc gacaatctcg atccagctga
     8461 tgttctgctg ttcaatcttc agtttgaaga gcgcggcggc gcggagttat tcgatccggc
     8521 ggaagactgg caggaacatg ttgatttcga tttgaaccca gacttttttg ccgaagttgt
     8581 gattggtctg gcggacagtg aagatggtga aatcaacgat gttttcgccc gcattctgtt
     8641 atgccgcgaa aaagatcaca aactttgcca tatcatctgg cgggaataaa taatcacttt
     8701 cggcgtgaaa aagggctgac agctgtcagc cctttttgtg tttaaagcgg atcgactttc
     8761 aggcaagaaa cggcgtgtct gaaactcccc tccagcacag gacgtgtttt ggcacactcc
     8821 ggaccggcaa tcgggcaacg ggtacggaaa acacaaccgg aaggcgggtt gatcggcgac
     8881 ggtaattccc cttccagtaa ctggatggtt ttgttcttct ccagatccgg atcaggtatg
     8941 gggactgccg acatcaatgc cctggtgtaa ggatgtagtg gattgtggta gacctcatca
     9001 taggtcccca gttctaccgc atggccgaga tacatcacca acacacgatc ggaaatgtgt
     9061 tttaccacgg ccaggtcatg agcgataaaa attaatgaca atcccatctc acgttgcaat
     9121 tgctggagca ggttgaccac ctgtgcctga attgacacgt ccagcgccga caccggctca
     9181 tcacagataa tcagcttcgg ttcaagaata agagcacgcg caatcccgat acgctggcac
     9241 tgcccaccag agaactcatg cggatagcgg ttaatcaggt taggcaataa cccgactttc
     9301 agcatcatcg ccttcacgcg ctcgcgaact tcctggcgtg acattttcgg atgataagta
     9361 cgcagtggtt cagcgatgat ctcgccgatg gtcatacgcg ggtttagcga tgccaacgga
     9421 tcctggaaaa tcatctgaat atcactgcga acggcacgcc attcatcggg tttcatgccc
     9481 agcaactctt tacctaacca ggcaacatga ccgtcggtcg ctttaaccag gccaatgatg
     9541 gcgcgagcaa aggtggactt accgcatccc gattccccta ccacacctaa tgtttcccct
     9601 tcatacaggc gaagagttac accatctacg gctttgagcg ttttcggcgg ttgccagaac
     9661 cactgtttgc catctttgat ttcaaagtgc actttcagat cggcgatttc gaggaggact
     9721 tttcttcctt cggttacagc attcataaca gttcctccac cggtttaaag caagcacgca
     9781 gacggccagg cgtaaactct tccagcggcg gtgcgctact acaaatttcc atcgcatgcg
     9841 gacaacgtgg ctggaatggg caacctttcg gtaatcgcag caggtttggc ggattacccg
     9901 gaatagtcaa cattgtttca ccttccgcat cgagacgagg caccgcgttg agcaaaccga
     9961 tagaataagg atgaacgggt tgataaaaga catcgcgcgc gttgccatat tccatcgtgc
    10021 gcccggcgta cattaccagc actttgtcgc agatccccgc caccacgcca agatcgtggg
    10081 tgatcataat gatggcggta ttaaattccc gcttcagttc attcaatagc gtcatgatct
    10141 gcgcctgtac ggtgacgtcc agcgcagtag tgggttcatc cgcaatcagc agcttaggtc
    10201 gacatagcaa tgccatcgca atcataactc gctgacgcat gccgccagaa aattcgtgcg
    10261 ggtacatttt catgcgttta cgtgcttccg gcatttttac cgcatcgagc atccgcaccg
    10321 actcttcaaa cgcctcagct ttgctcatgt tcttatgcag catcagcact tccatcaact
    10381 gctcaccgac gcgcatatag ggattcaacg aagtcattgg gtcctgaaaa atcattgaga
    10441 tttgttcagc gcgcagttta ttgagttcac gctctggcaa attgaggatt tcacgcccat
    10501 tgaaggttgc cgatccgcca atgcgtccgt tggcagccaa caggcccatc aacgcaaatg
    10561 cagtttgcga tttacccgaa ccagactcac ctacaatgcc cagcgtttct ccggcacgta
    10621 gggaaaaatt taaatcattg accgccgtga cgtcgccgtc cggggtacta aaggtgacac
    10681 gcaaatcttt cacgttcagc agtgcgtcag cctgttgttg tgcgagcggc acagttgcag
    10741 tttcaattac gctcatggct gcactcctta acgatctttc gggtcgaggg catcacgcaa
    10801 gccatcgccg ataaagttga aacaaaacag cgtcaccacg aggaatcccg ctgggaacaa
    10861 cagtaaccat ggagagactt ccattgagtt cgcgccatca ctcagcaatg ccccccagct
    10921 gcttaacggc tcttgcgtac ccaaccccag gaagctaagg aaagattcaa agaggatcat
    10981 gctgggcacc agtagcgatg cgtagaccac caccacaccg agtacgttcg gcacaatgtg
    11041 gcgaataaca atgcccgacg tcgatacacc gccaacttgt gccgcctcaa taaactcttt
    11101 gcgcttcaga ctcagggttt gcccacgcac aatacgagcc atatccagcc aggaaaccat
    11161 gcctatcgcc acgaaaatca gcaggatgtt ttgaccgaaa aaggtcacca gcaaaatgac
    11221 gaagaacatg aatgggaagg agttgaggat ttccagcaaa cgcatcatta ccgaatccac
    11281 tttaccgccc agataaccgg aaagcgaacc ataaagtgtc cccacgacca ctgccaccag
    11341 tgccgcagca acaccgacca tgagtgagat acgtccgcca atcgcaacgc gcacaagcag
    11401 gtcgcgaccg gatgagtcag taccaaagta gtgaccggac tccatatccg gggcgctgga
    11461 catcatcgcc cagtcagtat cgtcataggc aaactgcgaa agcatcggtg ccaggattac
    11521 aaataacgcg atcagcacca gcactatcag acttgcaacc gccgcacggt tatgcataaa
    11581 acgtcgacgt gcgtcctgcc acaagctgcg cccttcgacc tccagctttt cactgaaatt
    11641 ttccagcgtc tcgctgtttt tcttacttaa catcatcgcg agctccagta tcagtaacgg
    11701 attttcgggt cgataaccgc atatagcaca tcgacaatgg cattaaacaa aatggttaac
    11761 gcaccaacca ggatggtcag gcttaacact aaggaatagt cacggttcaa tgcgccatta
    11821 acgaacagtt gcccaatccc cggcaaacca taaatggttt cgataaccat agaaccggta
    11881 ataatgccga caaatgcggg gcccatatag gagagcacgg gtaacagagc aggttttaat
    11941 gcgtggcgta aaatgatccg ccgcataggt aaccctttcg cccgggcagt acgaataaag
    12001 ttggagtgta atacttcaat catagagcca cgggtaatac gcgcaatact ggcgatatag
    12061 gcgagtgaca acgccaccat cggcaatatc atgaatttaa gcgccccacc attccagcca
    12121 ccgcccggca gccaatgcaa aatgatcgca aatatcatga ctaataatgg cgcaaccaca
    12181 aaactgggga taacaacccc ggtcattgcc agccccatca cggtatagtc ccatttggtg
    12241 ttttgtttta atgcggcaat aacgccagca ctaacaccca gtattaccgc aaggaaaaat
    12301 gctgcggctc ccagtttggc agaaacggga aaactggatg caaccaggtc attgaccgaa
    12361 taatctttat atttaaacga tggaccgaaa tcgccatgcg ccagttgttt caggtagctg
    12421 aaatactgtg tcatgatcgg atcattaaga tgatatttcg cttcgatatt ggccattact
    12481 tccggcggta aagtacgttc gccggtaaaa gggcttcccg gcgcgaggcg catcataaag
    12541 aacgaaatag taataagaat aaatagcgtc ggaatcgctt ccagacagcg acgtagaata
    12601 aattttaaca ttgccctacc ttctggcctg tgccttctgt aatgcgataa aaatcagaca
    12661 ccgtggagca ggacactcct gccccacgta ttgccatcag tgcttcacaa tgtacatatt
    12721 ccgggtgtag gtattatcca gcggatcttt gccggtataa ccaccaaccc acggtttcac
    12781 cagacgcgca ttcacgtagt aataaacagg aacaatggcc gaatccttat ccagctgttg
    12841 ttctgcttta gtgtacagag ctgtgcgctg cgcctcgtca gtcactttca gcgtttccgc
    12901 cataatgctg tcaaaggccg ggctcttata atgcgcggta ttcatcgagc tgttcgacag
    12961 catggtgttc aggaaggaag ttggttcgtt gtagtcagca caccagcctg cacgggccac
    13021 atcaaaagta ccctggtgac gggtgtcgag gaacgttttc cactcctggt taaccagttt
    13081 gacgtttaca ccaatgtttt tcttccacaa tgaagaggca gcaatcgcca gctttttatg
    13141 cagatcggag gtgttataca acaggttgat ggtcaacggt ttgtctgcgg tataacccgc
    13201 ttcagccagc agttttttcg cttcttcgtt acgtttttcc tggctccagc caaaccattc
    13261 cggctgagtc aattttgcgc catcagtata cggtggagtg taaccatagg cgggcatgtc
    13321 gccctgcgct ttcactttat taacaatgat atcgcggtcc atacccagtt tcagtgcggt
    13381 acgcacacgc acatcgttga atggcggttt ctggttgtta atttcgtaat aataagtgca
    13441 caggtatgga tcaacgtgaa cttcgtccgg gatctctttt ttcagcttct ggaacaattc
    13501 gatcggcatg ttgttataag tcatgtcgat ttcaccacta cggtagcggt tgacatcggt
    13561 aacttcagaa gcaatgggca aataggttac ctggttaata acggttttcg cgttgttcca
    13621 gtaggttggg ctgcgttcaa gaacgattcg ttcgtttacg acccaatctt ttaaggtata
    13681 ggcaccgttg gtgacgatat taccaggctg ggtccatttt tcgccgaatt tctcgatagc
    13741 ggcttttggc accggtgaag ttgatgggtg aacaagtaat ttatagaagt acggaacggg
    13801 ttcacttaag gtgacttcta atgtgtgatc atcaatagct ttcacgccga gatcggtaat
    13861 cggttttttc ccttcaagaa tttcatcaat accggcgata tgcccatatt gcagataact
    13921 ggcatacgga gaagcggtgt tcggatcaac agaacgttgc cagctataca caaagtcttg
    13981 tgctgtgact ggcgtgccat cagaccattt cgcatcttta cgcaaatgga aggtccagac
    14041 tttcgcgtct ttattatccc aggattcagc gacgccaggt gccggatggc cgtcaagatc
    14101 gctgaccagt aagccttcaa acaggtctcg gctgatatta gactccggaa caccttcaat
    14161 tttgtgcgga tctaatgact gaacttctga accattgtta cgtaccagcg tttgtttttc
    14221 cgccagtgtg acgcctgcgg gtacatcagc tgccagcgcg acattccctg ccattagcgc
    14281 agccagaacg ccagctgcta ctaaacttct cttggtgatg ttggtcattg ttttttggac
    14341 tccctcatta taattactgg tattaaccag catgtgtaat cccggattgg gaccctgcac
    14401 aggctcggag actttctgct gtcaggttac tttatcgtta cggattgcta tcaccgactt
    14461 tatttattct ggtgattcag acgatcacct tatgtcgatt cgtactgtgt ctcccctgtc
    14521 gagacacatt attctttcgc gacgctcttt aattgttaat tattctcatt accaataaag
    14581 aatcgcctga cggttgcccg gaaagtacca aatgcggttc ttactcgcca atacattttg
    14641 caaaattgtt aagcaattct cttttattga ccgtatcaga atctatagct tgcatccatg
    14701 tcttaacaaa catctttaaa ttcaatcaaa tagagattgg ttaagcatgt cgatcttttc
    14761 tgattacagt t
//
LOCUS       DAZFBR010000115        14663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFBR010000115 DAZFBR010000000
VERSION     DAZFBR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.115"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            125..328
                     /locus_tag="ACHJZ2_003886"
     CDS             125..328
                     /locus_tag="ACHJZ2_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO4569915.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..1369
                     /locus_tag="ACHJZ2_003887"
                     /pseudo
     CDS             328..1369
                     /locus_tag="ACHJZ2_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1397..1486)
                     /locus_tag="ACHJZ2_003888"
     tRNA            complement(1397..1486)
                     /locus_tag="ACHJZ2_003888"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1450..1452),aa:Ser,seq:cga)
     gene            1580..2377
                     /gene="mtfA"
                     /locus_tag="ACHJZ2_003889"
     CDS             1580..2377
                     /gene="mtfA"
                     /locus_tag="ACHJZ2_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO4569916.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2478..2553
                     /locus_tag="ACHJZ2_003890"
     tRNA            2478..2553
                     /locus_tag="ACHJZ2_003890"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2511..2513,aa:Asn,seq:gtt)
     gene            2867..10050
                     /locus_tag="ACHJZ2_003891"
                     /pseudo
     CDS             2867..10050
                     /locus_tag="ACHJZ2_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(10312..11364)
                     /locus_tag="ACHJZ2_003892"
     CDS             complement(10312..11364)
                     /locus_tag="ACHJZ2_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO4569917.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            11678..12994
                     /gene="shiA"
                     /locus_tag="ACHJZ2_003893"
     CDS             11678..12994
                     /gene="shiA"
                     /locus_tag="ACHJZ2_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO4569918.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            13096..14550
                     /gene="amn"
                     /locus_tag="ACHJZ2_003894"
     CDS             13096..14550
                     /gene="amn"
                     /locus_tag="ACHJZ2_003894"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO4569919.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4030 a         3463 c         3510 g         3660 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tgtttttatc gcaatggaaa ttgcctatct gtgtgcagcg
      901 agggtttccg atgtgttatc gctgaaatgg gagcagattg gaaacgacgg gatctttatc
      961 cagcaaggga aaacagggaa aaaacagata aaagcatgga gtccacgatt acaggcggcg
     1021 atcgaaaaag caaaacagtt accaacatcc gcctatgtaa tcagcaatca atacggcaac
     1081 cgatatatgt acaaaggctt taacgaaatg tgggtagaag caagaaatca cgcaggcaaa
     1141 atttcaggta ttttaaccga cttcaccttt catgatctga aggcgaaagg aatttcagac
     1201 tatgaaggaa gcagccggga taagcaactt ttctctggtc acaaaaccga agggcaagtg
     1261 ctaatctatg acaggaaggt taaagtttca ccaacacttg atgtcccgtt acctgaaaat
     1321 attccaagaa aatattccaa gtaattccaa gtgtgatttt tgtcactgac ttaatgatgt
     1381 gtaagtgatt gaattttggc ggagagaggg ggatttgaac ccccggtgga gttgccccca
     1441 ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc agatggttgc
     1501 catgatgcca ggaaatttgg cattttaaca gtccctgtcc gtgcaatttt gttcaagtga
     1561 cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat cagcacatca
     1621 aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgtgtc tgacggaaca
     1681 ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc ggcttgttcc
     1741 tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc tattttgcct
     1801 acccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct taatttatcc
     1861 tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc ataaccaacg
     1921 tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact ggttggatat
     1981 acaagattct tttgatgctt ctggttttaa cctgattatt catgaagtcg ctcataagct
     2041 ggacacccgt aacggcgatc gcgccagcgg agttcccttt attccgttgc gtgaggttgc
     2101 tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa tcgaattggt
     2161 tggcgagaat gcggcgagca ttgatgctta tgctgccagt gatcctgctg aatgttttgc
     2221 cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt tcccttcatt
     2281 gtggcaacgt ttctgccaat tttatcaaca agatcctttg cagagactgc atcacgctaa
     2341 tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc agattaatta
     2401 accaattgaa atgacttatg aaatttagtg ttgacagaca aggtaccgct aagtaatatg
     2461 cgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
     2521 atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct tttttagcgc
     2581 aatgtcactg accttagttg aacattgttt tttaacggat agcgggtttt taacatctta
     2641 agcgccctcg acctttatgg ttgagggcgt tttgctatga acgccatcac cattttcccc
     2701 tcgattataa aacttgagtt attcagtagt ctcccctctt gcaactcaca tccaaaactg
     2761 cctaacgaaa agttattaat tttcaatcat attgctatca ggatttacat tttttcgctg
     2821 tgctagaaag ggcgcattta tgttagctcg ttcagggaag gtaagcatgg ctacgaagaa
     2881 gagaagtgga gaagaaataa atgaccgaca aatcttatgc gggatgggaa ttaaactacg
     2941 ccgcttaact gcgggtatct gcctgataac tcaacttgtg ttccctatgg ctgcggcagc
     3001 acaaggtgtg gtaaacgccg caacccaaca accagttcct gcacaaattg ccattgcaaa
     3061 tgccaatacg gtgccctaca cccttggagc gctggaatcg gcccaaagcg ttgccgaacg
     3121 tttcggtatt tcggtggctg agttacgcaa actcaaccag tttcgtacgt ttgctcgagg
     3181 ttttgataat gtccgccagg gtgatgaact ggatgtcccg gcacaagtta gtgaaaataa
     3241 tttaaccccg ccaccgggta atagcagtgg caaccttgag caacagatag ccagtacttc
     3301 acagcaaatc gggtctctgc tcgccgagga tatgaacagc gagcaagcgg caaatatggc
     3361 gcgtggatgg gcctcttctc aggcttcagg cgcaatgaca gactggttaa gccgcttcgg
     3421 tacctacaaa ccaaatatgc cctgaaaggc tataacgtcg aagccaccgc tctggaagct
     3481 gccggtggta aagtggttac aacgggtaaa gatattctgg ttaccctgcc ggcgtaccgg
     3541 ttcaccagta cgccagaaac cgataacacc tggccgattg aagtcaccgc tgaagatgtc
     3601 aaaggcaatt tttcgaatcg tgaacagagc atggtagtcg ttcaggctcc tacgctaagc
     3661 cagaaagatt cctcggtatc gttaagtagc cagacgttga gcgcggattc ccattcaacc
     3721 gccacactga cttttattgc gcatgatgca gcaggtaatc ctgttatcgg gctggtgctc
     3781 tcgacgcgtc acgaaggtgt tcaggacatc accctttctg actggaaaga taatggtgac
     3841 ggaagctata cccagatcct gaccacagga gcgatgtctg gcacgctgac gctgatgcca
     3901 cagctgaacg gtgtggatgc ggctaaagcc cccgccgtgg tgaatatcat ttctgtttcg
     3961 tcatcccgga ctcactcgtc aattaagatt gataaggacc gttatctctc cgggaatcct
     4021 atcgaggtga cggtagaact gagagatgaa aatgacaaac ctgttaagga gcaaaaacag
     4081 caactgaata ccgcagtcag catcgacaac gtgaaacctg gtgtcactac agactggaaa
     4141 gaaaccgcag atggcgtcta taaggcaacc tataccgcct ataccaaagg cagtgggctt
     4201 actgcgaagc tgttaatgca aaactggaat gaagatttgc ataccgctgg atttatcatc
     4261 gacgccaacc cgcagtcagc gaaaattgcg acattatctg ccagcaataa tggtgtgctc
     4321 gccaatgaga atgcagcaaa caccgtctcg gtcaatgtcg ctgatgaagg aagcaaccca
     4381 atcaatgatc ataccgtcac gtttgcggta ttaagcggat cggcaacttc ctttaacaat
     4441 caaaacaccg caaaaacgga tgttaatggt ctggcgactt ttgatctgaa aagtagtaag
     4501 caggaagaca acacggttga agtcaccctt gaaaatggcg tgaaacaaac gttaatcgtc
     4561 agttttgtcg gcgactcgag taccgcgcag gttgatctgc agaagtcgaa aaatgaagtg
     4621 gtcgctgacg gcaatgacag tgccacaatg accgcgacag ttcgggatgc aaaaggcaac
     4681 ctgctcaatg acgtcaaggt caccttcaat gtcaattcag cagcagcgaa actgagccaa
     4741 accgaagtga atagccacga cgggatcgcc acagctacgc tgaccagttt gaaaaatggt
     4801 gattatacgg ttacggcctc tgtgagctct ggttctcagg ctaatcaaca ggtgattttt
     4861 atcggtgatc aaagtactgc tgccctgacc ctcagtgtgc cttcaggtga tatcaccgtc
     4921 accaacacag ctccgctaca tatgactgca accttgcagg ataaaaatgg caatccacta
     4981 aaagataaag aaatcacctt ctctgtgcca aacgacgtcg caagtcggtt ctcgattagc
     5041 aacagcggaa aaggcatgac ggatagcaac gggactgcaa tcgcctccct gaccggcacg
     5101 ttagcgggca cgcatatgat cacggctcgt ctggctaaca gcaatgtcag cgatacacag
     5161 ccaatgacgt ttgtggcgga taaagacaga gcggttgtcg ttctgcaaac atcgaaagcg
     5221 gaaatcattg ggaatggcgt ggatgagacg actctgacag caacagttaa agatcctttt
     5281 gataacgtgg ttaaaaatct ttcagtagtc ttccgcacct cccccgcaga cacgcaactg
     5341 agtctgaacg cgcgtaatac taatgagaac ggtattgccg aagttaccct taagggcacg
     5401 gttttgggtg ttcatacagc cgaagccata ctgcttaacg gcaacagaga tacgaaaatc
     5461 gtcaatattg cgcccgatgc cagcaacgcg caggtcaccc tgaacatccc tgcacaacag
     5521 gtggtgacga ataacagtga cagcgtgcag ctgacggcga cggtgaaaga cccgtcgaat
     5581 catccggtgg cgggaataac ggtgaacttc accatgccac aggacgtggc ggcaaacttt
     5641 acccttgaaa ataacggtat tgccatcact caggccaatg gcgaagcgca tgtcaccctc
     5701 aaaggcaaaa aagcgggcac gcatactgtg accgccacgc tgggtaacaa taatgccagc
     5761 gatgcgcaac cagtcacctt cgtggcggat aaggacagcg cggttgtcgt tctgcaaaca
     5821 tcgaaagcgg aaatcattgg gaatggcgtg gatgagacga ctctgacggc aacagtgaaa
     5881 gatccttttg ataacgcagt aaaagatcta caggtcacct tcagtaccaa ccccgcagat
     5941 actcaactta gtcagagcaa aagcaatact aacgacagtg gtgtggccga agttaccttt
     6001 aagggcacgg ttttgggtgt tcatacagcc gaagccacac tgcctaacgg caacaacgat
     6061 acgaagatag tcaatattgc gcccgatgcc agcaacgcgc aggttacgct gaacatccct
     6121 gctcaacagg tggtgacgaa taacagcgac agcgtgcagc tgacggcgac ggtgaaagat
     6181 ccgtcgaatc atccggtggc gggaataacg gtgaacttca ccatgccaca ggacgtggcg
     6241 gcaaacttta ccctcgaaaa taacggtatt gccatcaccc aggccaatgg ggaagcgcat
     6301 gtcacgctca aaggtaaaaa agcgggtacg catacggtta ccgcaacgct gagtaataac
     6361 aataccagtg attcacagcc ggtaacgttt gtggcggaca aaacctcggc tctggttgtt
     6421 cttcagatat caaaaaatga gatcacaggt aatggcgtcg atagcgcaac gctaactgca
     6481 acggtcaaag atcagttcga caatgaggtg aacaatcttc cggtaacatt cagcacagct
     6541 tcttcaggcc tcaccctgac cccaggggaa agtaatacca atgagtctgg catcgcgcag
     6601 gccactctcg caggcgttgc ctttggtgag cagacggtca ctgcatcact ggctaataat
     6661 ggtgccagcg acaacaaaac tgtgcatttt attggcgaca cagcggcggc aaaaattatc
     6721 gagttgacgc ctgtcccaga cagcataatc gcaggtaccc cgcagaacag ctccggcagc
     6781 gtcatcaccg ccacagtcgt tgataataat ggctttccgg tgaaaggtgt gactgtgaac
     6841 ttcaccagca acgcagcgac agccgaaatg acgaatggcg gtcaagccgt gacgaacgaa
     6901 cagggtaagg ctaccgtcac ttataccaat acccgctcct cgatagaatc aggagcgaga
     6961 ccggataccg ttgaggccag tctggaaaat ggtagctcca cgcttagcac atcaattaat
     7021 gtcaacgctg atgcgtctac ggcacatctc accttgctac aggcactttt tgatacagtc
     7081 tccgcaggcg acactaccaa tctgtatatt gaggtgaagg ataattacgg caacggagta
     7141 ccccagcagg aggtaaccct cagcgtttca ccaagtgaag gtgtgacccc cagtaataac
     7201 gctatatata cgaccaatca cgacggcaat ttttacgcaa gctttaccgc tacaaaagcc
     7261 ggggtatacc aagtgacggc aaccctcgaa aatggcgatt cgatgcaaca aacagtgacc
     7321 tatgtgccga acgtagcgaa tgctgaaatc tcgctggcag cctcgaagga tccggtaatt
     7381 gccaacaata acgatctcac gacactaaca gcaacagtcg ctgatacaga gggcaatgcg
     7441 atagccaaca gtgaggtaac atttactctg ccggaagatg tgagggcgaa cttcacgctg
     7501 ggcgatggcg gtaaagtggt tactgatact gaaggcaaag cgaaagtcac gctgaaaggt
     7561 acaaaagcag gcgctcatac tgttacagca tcgatggctg gcggtaagag tgagcagttg
     7621 gtggtgaact ttattgcgga tacactcact gcgcaggtta atcttaacgt taccgaggac
     7681 aattttatcg ctaataacgt cgggatgacc aggctgcagg caacagtgac tgatggaaac
     7741 ggcaacccgt tagccaatga ggcggtgaca ttcacgctac cggcagatgt gagcgcaagc
     7801 tttactctcg gacaaggcgg ttccgccatt actgacatca acggcaaggc tgaagttaca
     7861 ctgagcggta caaaatccgg cacctacccc gtgacagtta gcgtgaacaa ttatggtgtc
     7921 agtgatacga aacaggtgac tttgattgcc gatgctggta ccgcaaaact agcctcctta
     7981 acctctgtat actcattcgt cgtcagcacg accgagggcg cgaccatgac tgcaagcgtc
     8041 actgacgcta acggcaaccc ggtagaaggt ataaaagtta atttccgcgg aacttccgtc
     8101 acgctaagca gcaccagcgt tgaaacggat gatcggggtt tcgctgaaat tcttgtgaca
     8161 agcaccgagg tcggactgaa aacagtttca gcctctctgg cagataaacc tactgaagtc
     8221 atctcgcgat tactgaatgc aaaagcagat attaattctg caacgattac cagtctggag
     8281 atacctgaag gtcaggtcat ggtcgcacaa gacgtagcag ttaaagctca cgtcaacgac
     8341 cagtttggca atccgattct taatgaatct gtaacattca gtgcagaacc accagagcac
     8401 atgaccatca gccaaaatat tgtctctact gatacgcatg gtatagccga ggtcactatg
     8461 acgcccgaaa gaaacggttc gtatatggtg aaagcatccc tggcgaatgg atcctcttat
     8521 gagaaggatc tggtggtaat cgatcaaaaa ctgacactct cggcgtccag cccgcttatc
     8581 ggtgtcaatt ccccaacagg tgcaactctg acggcaacgc taacttctgc aaatggcact
     8641 ccagtggagg gtcaggtcat caactttagc gtaacgccag aaggtgcgac gttaagtggc
     8701 ggaaaagtga gaaccaactc ttcaggtcag gctccagtcg ttctgaccag caataaagtc
     8761 ggtacatata cggtgactgc atcgttccat aacggcgtaa caatacagac acagacaatc
     8821 gtgaaagtca ctggcaactc aagcaccgcc catgttgcta gctttatcgc tgatccatcg
     8881 actatagccg ccaccaacag tgatttaagt accttaaagg caacggttga ggatggcagt
     8941 ggtaacctga tcgaaggtct cactgtgtac ttcgccttaa aaagcggctc tgccacatta
     9001 acgtcattaa cagcggtgac agatcaaaac ggaatcgcga caacaagcgt gagaggagcg
     9061 ataacgggga gcgtcacggt aagcgcagtc acgaccgctg gtggaatgca aacagtagat
     9121 ataacgctgg tggcaggccc ggcagacgcc tcgcagtccg tccttaagaa caatcggtca
     9181 tcattgaaag gagactttac cgatagtgct gagctacatc ttgttctgca cgatatatca
     9241 ggcaatccga tcaaagtttc tgaagggctg gaatttgtgc agtcaggtac caacgcgccc
     9301 tatgtgcaag ttagtgcaat tgactacagt aaaaatttct caggcgagta caaagccact
     9361 gttacaggcg gcggagaggg tatcgcaacg ctgatccctg tattgaatgg tgttcatcaa
     9421 gcgggtctga gtaccacaat acaattcact cgcgcagaag acaaaataat gagcggtaca
     9481 gtgttagtca atggtgctaa cctaccgaca actacattcc cttcgcaggg gttcactggg
     9541 gcgtattatc agttgaataa tgacaacttt gccccaggaa aaacggcggc tgattatgag
     9601 ttttcaagct ctgcctcctg ggttgatgtt gatgctaccg gtaaagtgac atttaaaaat
     9661 gtcggcagca aatgggagag gattacggcg acgccaaaaa caggcggccc tagctatata
     9721 tacgaaatcc gagtgaagag ttggtgggtg aacgccggcg atgctttcat gatatacagc
     9781 cttgctgaaa atttttgcag tagcaatggc tacacacttc cccttggaga ccatttaaac
     9841 catagtcgtt cccgaggcat cgggtcactg tacagtgaat ggggagatat ggggcattac
     9901 acgactgaag ctggttttca ttcaaatatg tattggtcat cgagtcccgc aaactcaaac
     9961 gaacaatacg tagtttccct ggcaacaggt gatcaaagcg tatttgaaaa gcttgggttt
    10021 gcttatgcga catgttataa aaacctctga ttttccgtta taatttctta aagagtgctc
    10081 cattcggggc actctttttt acattccctc tatagagctt gcccttctct cacagaaaat
    10141 gaataaataa aaatgcgata ccgcaagaat cgcgttcgat gtgactatga tcgtatccgg
    10201 tgagtacagc ttttatccaa aggctggttt tctgtgatga ttgaccagcc tcagagtatt
    10261 gatgacacca ccgttcataa cagatcggca ttactatgca taagtactgt ctcatattct
    10321 cgccaaaaca ctttttaatg cctttgcgaa ttcatccaga cttatattgt caattttttg
    10381 cccaacgacg actgactccg aaacatcggc cattgcgggc catcgcgttt tgtaatcagc
    10441 ataaattgca acaacggggg tattataggc acttgccata tgtaatattg aggtgtcagg
    10501 agttattgcg atataagcat cttttatgat tgctgctgaa cgtttaatcg agggtgataa
    10561 ggataaacgg tagacattat tataacaatc cactaatacc ctggctttat cttccccttt
    10621 tggtccatga acgatcacta ttggtatatc tgtttcactt tgaatttttt ctattagatt
    10681 ttctgcaatc gatagtgaaa atgtacgatc ctgcgagcta ccttctaaat taagcgcaat
    10741 gtaagatcct aacgatcgca tttcctcgcg cacctcatcg agtacatcct cacttagtgg
    10801 caattcatat attggcctga ctgctggaaa acccgcctca cgcatcataa atgcccatgt
    10861 catgggtacg ggagcccgga gtttctgatc catccgggac gcgttcttac acacagggga
    10921 atagcacttc atggttaaac caacaacctg aaaattcgtt tttgctttca actgactgat
    10981 aaataacatc gttttcaggt tctttttacg catcgcctct atacaaagat ccggcgtacc
    11041 gtattgctgt gttatgttct tcgctaaatc ttttatttct tttaatgttg cgtgatcctg
    11101 catagtcatt gtgactaatg ttaatttagt ctgttcaagt ttgagcgcat taaagacttc
    11161 taaattaatt gtcgacgtaa caattaatag atgcttaatt ttatgcaatt caagcgcccg
    11221 aataacagga aagatggcca tagcatcgcc aatctgatcg ggaatatgga tgacaacaaa
    11281 gtctgttttt tcaatattga aattataagc tttataatcg tagtaactaa atgcaatacg
    11341 tctcaacaat gatactaaaa acataactaa cctcgcctcc ctactggtta taatgcaatg
    11401 cagtctatca gaatcatcag ggtgcaattt tgtgcatatg cggactttta tgtttcatat
    11461 ctcgaacctg tggggcctct gcttaatcct taaacaacac cagcaactcc tgcgctttca
    11521 tcttccagcg aatttttcat gttgccgtta atcagccata aaatcatttg cagatgcgct
    11581 ctgtcgaggt agtctcataa ggttcgttta tagatcgacg caatgtgagt taccttttcc
    11641 atactaatta taaaaagaca gtacaaacag gatcattatg gactccacgc tcatctccac
    11701 tcgtcccgat gaaaggacgg tttcgttaag tcgcgcccga cgagctgcgt taggcagttt
    11761 cgctggtgcc gtcgtcgact ggtatgattt tttactctat ggcattaccg ccgcactggt
    11821 gtttaatcgc gagtttttcc cgcaagtaag cccggctatg ggaacgctcg ccgcatttgc
    11881 cacctttggc gtcggattcc ttttccgtcc gctcggcggt gtcattttcg gtcactttgg
    11941 cgaccgactg ggacgtaagc gcatgttaat gctgaccgtc tggatgatgg gcatcgcgac
    12001 agccttgatt ggtattcttc cttcattctc gaccattggg tggtgggcac ctattttact
    12061 ggtgacgctg cgtgccattc agggatttgc cgtcggcggc gaatggggag gcgcggcgtt
    12121 gctttccgtt gaaagtgcac cgaaaaataa aaaagccttt tacagtagcg gtgtacaagt
    12181 tggctacggt gtaggtttac tgctttcaac cggactggtt tcattgatca gtatgatgac
    12241 gactgacgaa cagtttttaa gctggggctg gcgcattcct ttcctgttta gcatcgtact
    12301 ggtactggga gcattgtggg tgcgcaatgg catggaagag tccgcggaat ttgaacaaca
    12361 gcaatataat caagcggccg cgaaaaaacg catcccggtt atcgaagcgc tgttacgaca
    12421 tcccggtgct ttcctgaaga ttattgcgct acgactgtgc gagttgctga caatgtacat
    12481 cgttaccgcc tttgcactta attattcaac tcagaatatg gggttaccgc gcgaactttt
    12541 ccttaatatt ggtttgctgg taggtggatt aagctgcctg acaattccct gttttgcctg
    12601 gcttgccgat cgttttggtc gccgcagggt ttatatcaca ggcgcgttga tcggaacgtt
    12661 gagcgcattt cctttcttta tggcgcttga agcacaatct attttctgga tagttttctt
    12721 ctccataatg ctggcaaaca ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt
    12781 taccgaaatg tttggtgcca gttatcgcta tagtggtgct ggagtcggtt atcaggttgc
    12841 cagtgtggtt ggcggtggat ttacaccttt tattgccgct gcgctcatca cttactttgc
    12901 cgggaactgg catagcgtcg ccatttattt gctggctgga tgtctgattt ccgcaatgac
    12961 cgctttgttg atgaaagaca atcaacgcgc ttgatagtct ggcgaagatc atccgatctt
    13021 cgccttacac ttttgtttca cattcctgtg acatactatc ggatgtgcgg taattgtatg
    13081 gaacaggaga cacacatgaa taataagggc tccggtctga ccccagctca ggcactggat
    13141 aaactcgacg cgctgtatga gcaatctgta gtcgcattac gcaacgccat tggcaactat
    13201 attacaagtg gcgaattacc tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca
    13261 tctctgaccg taacctggga cggtagcaca acaaatcccc ccaaaacgcg cgcatttggt
    13321 cgttttactc acgcaggcag ctacaccacc acgattactc gccctactct ctttcgttcg
    13381 tatcttaatg aacaacttac gttgctgtat caggattatg gcgcgcatat ctcggtgcaa
    13441 ccctcacagc atgaaatccc ttatccttat gtcatcgatg gctctgaatt aacacttgat
    13501 cgctcaatga gcgctgggtt aactcgctat ttcccgacaa cagaactggc gcaaattggc
    13561 gatgaaactg cagacggcat ttatcatcca actgaattct ccccgctatc gcattttgat
    13621 gcgcgccgcg tcgatttttc cctcgcgcgg ttgcgccatt ataccggtac gccagttgaa
    13681 cattttcagc cgttcgtctt gtttaccaac tacacacgtt atgtggatga attcgttcgt
    13741 tggggatgca gccagatcct cgatcctgat agtccctaca ttgccctttc ttgtgctggc
    13801 gggaactgga tcaccgccga aaccgaagcg ccagaagaag ccatttccga ccttgcatgg
    13861 aaaaaacacc agatgccagc atggcattta attaccgccg atggtcaggg tattactctg
    13921 gtgaatattg gcgtgggacc gtcaaatgct aaaaccatct gcgatcatct ggcagtgcta
    13981 cgcccggatg tctggttgat gattggtcac tgtggcggat tacgtgaaag tcaggccatt
    14041 ggcgattatg tacttgcaca cgcttattta cgcgatgacc acgttcttga tgcggttctg
    14101 ccacccgata ttcctattcc gagcattgct gaagtgcaac gtgcgcttta tgacgccacc
    14161 aagctggtga gcggcaggcc cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg
    14221 gtaaccacag atgacaggaa ctgggaatta cgttactcag cttctgcact tcgttttaac
    14281 ttaagccggg ccgtagcaat tgatatggaa agtgcaacca ttgccgcgca aggatatcgt
    14341 ttccgcgtgc catacgggac actactgtgt gtttcagata aaccgttgca tggcgagatt
    14401 aaacttcccg gtcaggctaa ccgtttttat gaaggcgcta tttccgaaca cctacaaatt
    14461 ggcattcggg cgatcgattt gctgcgcgca gaaggcgacc gactgcattc acgtaaatta
    14521 cgaaccttta atgagccgcc gttccgataa taagttctga accttcttat caaaaataga
    14581 tgtgaaggat taagtaagac atctggcaaa tagcccgcaa taaacgcggg ctgtttgctt
    14641 ccttgggcgg atacgagttt tat
//
LOCUS       DAZFBR010000116        14541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFBR010000116 DAZFBR010000000
VERSION     DAZFBR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.116"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(38..274)
                     /gene="yjjZ"
                     /locus_tag="ACHJZ2_003895"
     CDS             complement(38..274)
                     /gene="yjjZ"
                     /locus_tag="ACHJZ2_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO4569920.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            415..1203
                     /gene="fhuF"
                     /locus_tag="ACHJZ2_003896"
     CDS             415..1203
                     /gene="fhuF"
                     /locus_tag="ACHJZ2_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO4569921.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1241..1918)
                     /gene="bglJ"
                     /locus_tag="ACHJZ2_003897"
     CDS             complement(1241..1918)
                     /gene="bglJ"
                     /locus_tag="ACHJZ2_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO4569922.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1876..2601)
                     /gene="yjjQ"
                     /locus_tag="ACHJZ2_003898"
     CDS             complement(1876..2601)
                     /gene="yjjQ"
                     /locus_tag="ACHJZ2_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4569923.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3222..3992
                     /gene="yjjP"
                     /locus_tag="ACHJZ2_003899"
     CDS             3222..3992
                     /gene="yjjP"
                     /locus_tag="ACHJZ2_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO4569924.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            3983..4456
                     /gene="yjjB"
                     /locus_tag="ACHJZ2_003900"
     CDS             3983..4456
                     /gene="yjjB"
                     /locus_tag="ACHJZ2_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO4569925.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4563..5102
                     /gene="dnaT"
                     /locus_tag="ACHJZ2_003901"
     CDS             4563..5102
                     /gene="dnaT"
                     /locus_tag="ACHJZ2_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO4569926.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5105..5842
                     /gene="dnaC"
                     /locus_tag="ACHJZ2_003902"
     CDS             5105..5842
                     /gene="dnaC"
                     /locus_tag="ACHJZ2_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO4569927.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5891..6385
                     /gene="yjjA"
                     /locus_tag="ACHJZ2_003903"
     CDS             5891..6385
                     /gene="yjjA"
                     /locus_tag="ACHJZ2_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO4569928.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6639..8930
                     /gene="opgB"
                     /locus_tag="ACHJZ2_003904"
     CDS             6639..8930
                     /gene="opgB"
                     /locus_tag="ACHJZ2_003904"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO4569929.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9067..10089)
                     /gene="lgoD"
                     /locus_tag="ACHJZ2_003905"
     CDS             complement(9067..10089)
                     /gene="lgoD"
                     /locus_tag="ACHJZ2_003905"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO4569930.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10228..11142
                     /gene="lgoR"
                     /locus_tag="ACHJZ2_003906"
     CDS             10228..11142
                     /gene="lgoR"
                     /locus_tag="ACHJZ2_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO4569931.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            11357..12718
                     /gene="lgoT"
                     /locus_tag="ACHJZ2_003907"
     CDS             11357..12718
                     /gene="lgoT"
                     /locus_tag="ACHJZ2_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4569932.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(12767..14431)
                     /gene="tsr"
                     /locus_tag="ACHJZ2_003908"
     CDS             complement(12767..14431)
                     /gene="tsr"
                     /locus_tag="ACHJZ2_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO4569933.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
BASE COUNT         3634 a         3787 c         3425 g         3695 t
ORIGIN      
        1 ttatctttac ttcctttctt gtttcttccc tgttttttca tccctgattc aaattcatta
       61 tcatcagcat gatgccagca ataagcgcca ggcacgatgg tagcaaaatg taatgccgca
      121 actgtttgtg atacagcatc accggcgtca atactaatcc catcagaatg acaatccgcc
      181 actgatccgg cgataaatcc gcatacatca gccctgcaat cagaaatccc ggcagcaaca
      241 ctccccagcc actgcccagc gtacgttgca acatggtttt atctctttca ttgataatga
      301 taaccaatat catatgataa tttttatcac ttgcaagcca gagaaatccc ttgctatcgg
      361 gtaaacctat cgctaggatt agcaatcatt atcatttaga ttactatccc gattatggcc
      421 tatcgttccg caccgctcta tgaagatatc atctggcgaa cgcatctcca gccacaggat
      481 gctaggcttg cacaagccgt acgtgcgacg atcgccgaac atcgtgaaca tttgctggag
      541 tttatccgcc tggatgaacc cgctccgctt aacgccatga cgctggcgca atggtcatca
      601 ccgaatgcgc taagttctct actggcggtc tattccgatc atatctatcg caaccaacca
      661 acgatgatcc gcgagtacaa accgctgatc tcactatggg cacaatggta tatcggcctg
      721 atggtgccac cattaatgct ggcgctactg acgcaggaaa aagcattaga tgtgtcgccg
      781 gaacatttcc atgctgagtt tcacgaaacc ggacgcgtcg cctgtttctg ggtcgatgtg
      841 tgtgaagata aaaacgcaac accacattcg ccgcagcagc gaatggaaac gttaatcagc
      901 caggcgctgg ttccggttgt gcaggcacta gaagcgaccg gagagatcaa cggcaaactt
      961 atctggagta ataccggtta tttgatcaac tggtatctta ctgagatgaa gcaactgctc
     1021 ggcgaggcga cggttgaatc gctgcgccat gccctctttt ttgagaaaac gcttactacc
     1081 ggtgaagata atccactctg gcgtaccgtg gtgctacgcg acggcctgct ggtgcgccgc
     1141 acttgctgcc agcgttatcg cttaccggat gtacagcaat gtggcgattg tacgctgaaa
     1201 tgaaagcact gccggggaag taaacccggc atcatgcgga ttaataggga tgcaacacat
     1261 tacttgtttc gcaatgccgc atacataaca gaatatctgc ggcctccaac aaccctgcgt
     1321 cagaactgac gcccagtttc gacatcacat taaatttatg tgcacggatc gttttgatat
     1381 tgcgtttaag ctgctcggca atttgtgtca ttgagtagcc acgcgacata aagcgcaata
     1441 tttctctctc cgtcgggctt aacgtccggc tttggttaat gtaccactga ttgttcagtc
     1501 ggtcggtcgc ctgacgtaca ccatttaatg acaaaaagag ttcctgatga aaaatctcca
     1561 gcgttgacgc tttacttaat acaccgtcca gcggtgatgg cgacaatgaa ccaatcagcc
     1621 gagcttcaat atcatcatcc gcaataacta aacgccgggt acgcggaaac ttaatcgcca
     1681 gttcagtcag gcaagataat ccctctctgc gctcacttct catggcagaa aaagaaaaaa
     1741 tgaccgccgc aaacgagatc cgcgacatgg catcctgaaa tgactcctga ctggtatagg
     1801 tatggagctt ataagggttg cccgcaaact ttctgaataa actctcaata ccgatactac
     1861 tcatgacgca tttttctatg agtgcgacat ttctcttctt aattcggctg tgttccattc
     1921 tgcggcatct ccataaacag aaggcaagtt aagttctctt gcaccctgcg cgctatttat
     1981 ccatgcatac atttcagcgt tgctgtgaat tgccagtcga cgcatcgcac tgtttttttg
     2041 ggcgctgata gttttattac ttttcttaag caatgaagct atctggttga tgccccatcc
     2101 tttacctaat agccgcagta ccttgcgctc tgaaagagtg agaatgacgg agcgatagct
     2161 aaaatcatga gtttcagtca ttgccggaga ggtcagcatg gcgctgatac gctctgcgtg
     2221 cgtattgccg gaacgtacgg tcttgaccag attctcaatg ggttcaacat cagacaataa
     2281 cgtcgccgta gggcacatga gcagttctac agcctgggaa taccaagagc gtgacaccat
     2341 gaaaacccag tgaatttccc gatattgtga gattaaagaa taataatgtt cacaaacaga
     2401 acgtggatct tcactttcac cggctaaatc agcaatgact aatccggaac gacgtaattg
     2461 taataaggta aggtcctccg cagaggcgct ggatattatt tcatattcag ggaagtgagt
     2521 cctcatgacc tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt
     2581 tttgcagcat cctggcaaca tatttcctcc acatccactg tctaaaaatt atgatggttt
     2641 attcaggata ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta
     2701 tccttagcat tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg
     2761 tcagaaggtt agcatcaaat gataacctgg tgaaatagcc tggagacatt taaaatatct
     2821 acattaagtt gttagaaata cttaatgaaa aacagaaatc tcattcattt aatttataat
     2881 taaatgatat ctcttcccgc agaatgcggg aatcacacaa tcaatattta ttcaataaaa
     2941 tctgatgatt ttaacttgtt aacactaaaa aacgcgccca gctatctatt atcctgaccc
     3001 gtaaaggcta aaacaataaa ttcattatag tgataatatt catgtgatag tttgtttgcg
     3061 gcgagagata attcgctttt tatcaccgag cttgtcccga taaccaggat taatcagcgc
     3121 aattttgatc ttaacctacc gcaaaacaac cttgcttcat gatacaaatg gattctcatt
     3181 atctgaacaa tacgcagcac gtctatgaca aagggcgagt tatgcaaact gagcaacagc
     3241 gagccgtaac acggctttgt atccagtgtg gattatttct tttgcaacat ggtgcggaaa
     3301 gcgcgttggt tgatgagctt tcctcacgac tgggtcgggc gctgggaatg gacagcgtcg
     3361 aaagttctat ctcttcgaac gccatagtgc tgacaactat taaagatggg caatgcctga
     3421 catcgacacg taaaaatcac gatcgcggca ttaatatgca tgtggtgact gaagtccagc
     3481 acattgtgat tcttgcggag catcatctgc tggattacaa aggcgtagag aaacgattta
     3541 gccaaattca gccattacgt tacccaagat ggctggtagc cttaatggtt ggcctttctt
     3601 gcgcctgttt ctgtaaactc aataacggtg gctgggatgg tgccgtcatc accttctttg
     3661 ccagtacgac cgcgatgtat atccgccagc tgctggcaca acgtcatctt catccacaga
     3721 tcaacttttg ccttaccgct ttcgccgcca ccaccatttc cggattgctt ttgcaacacc
     3781 ccactttcag caataccccc accattgcga tggccgccag cgttctgctg ctagtgccgg
     3841 gctttccgtt gattaatgcc gtcgccgata tgtttaaagg ccacatcaat accggactgg
     3901 cacgctgggc gatcgccagt ctgctgacac tggctacctg cgtcggcgta gtgatggcac
     3961 tgacgatttg ggggctacgc ggatgggtgt gatcgaattt ctgttagcgt tggcgcagga
     4021 tatgatcctc gccgccattc ctgcggtcgg ctttgcgatg gtgttcaacg ttcccgtgcg
     4081 ggcgttacgc tggtgtgcgc tgcttggctc gataggtcat ggttcccgaa tgatcttgat
     4141 gaccagcggg ttgaatattg agtggtcaac ctttatggcc tccatgctgg tcggcaccat
     4201 tggtattcaa tggtcgcgct ggtatctggc gcatccgaaa gtgtttaccg tggcggccgt
     4261 tatccctatg ttcccgggca tatcggctta taccgcaatg atttcggcgg taaaaatcag
     4321 ccagttaggt tacagcgaac cgttgatgat taccctgtta accaactttc ttacagcttc
     4381 atcgattgtt ggtgcgttat ccatcggtct ttccattcct ggattatggt tgtaccgcaa
     4441 gcgccctcgc gtataaaatt gcctcgctta agcacacgga tgagagacag cctcctctcc
     4501 tccgtgtgtt actataaaag ttatctccct tctcgttcat cgttccatat ttgagaaaca
     4561 gtatgtcttc cagagttttg accccggacg tcgttggtat tgacgccctg gtacacgatc
     4621 accaaaccgt tctggcaaaa gctgaaggcg gtgtggttgc cgtatttgct aacaatgccc
     4681 cggcgtttta tgccgtcacg cctgcacgcc tggctgaact gctggcgctg gaagaaaagc
     4741 tggcgcgtcc gggaagcgat gtcgatctgg acgatcaact ctatcaggaa ccgcaagccg
     4801 ctcccgttgc tgtacccatg gggaaattcg ccatgtatcc ggactggcaa cccgatgccg
     4861 actttatccg cctggcggct ctatggggcg tggcgctaag agagccggta accaccgaag
     4921 aactggcctc attcattgcc tactggcagg cggaaggtaa agtctttcac catgtgcagt
     4981 ggcaacaaaa actggcgcgc agcctgcaaa tcggtcgtgc cagcaacggc ggactgccga
     5041 aacgagatgt gaatacggtc agtgaacctg acagccaaat tccaccagga ttcagagggt
     5101 aacgatgaaa aacgttggcg acctgatgca acgcctgcaa aaaatgatgc ctgcccatat
     5161 caaacctgca ttcaaaacgg gtgaagagct actggcctgg cagaaagaac aaggggcgat
     5221 ccgctccgcc gctctcgaac gtgaaaatcg ggcgatgaaa atgcagcgca cctttaaccg
     5281 ctccggtatt cgcccactgc atcagaactg ctcctttgag aactatcgcg ttgagtgtga
     5341 agggcagatg aatgcgttaa gcaaagcgcg ccagtatgtc gaagagtttg acggcaacat
     5401 cgccagcttc atcttttctg gtaagccagg aaccggcaaa aaccatctgg cggcggcaat
     5461 ctgcaacgag ctgctgctac gcggtaaatc cgtattgatc atcaccgtgg ccgatattat
     5521 gtcggcgatg aaagatacct tcaggaatag cggtaccagc gaagaacaac tgcttaacga
     5581 tctgagcaac gttgatctgc tggtgatcga tgagatcggc gtgcagaccg aatcgaaata
     5641 cgaaaaagtg atcatcaacc agatcgtcga tcgccgctct tcttccaaac gcccaaccgg
     5701 gatgctgacc aacagtaata tggaagagat gaccaagttg ctgggcgaac gcgtgatgga
     5761 ccgtatgcgc ctgggtaaca gtttgtgggt gatcttcaac tgggatagct accgtagccg
     5821 tgtaacaggt aaagagtatt aatccccgtt tctggcggta tactgcgaca caattccacc
     5881 ttgagtaatg atgatgaaaa ctgttaaaca tcttctgtgc tgtgccattg ccgccagcgc
     5941 attaatttcc accggggtgc atgctgcgtc ctggaaagat gcgctctcca gcgccgccag
     6001 cgaacttggc aaccaaaaca gcacgacaca ggaaggcggt tggtcgctcg cgtcattaac
     6061 taacttgctt agcagcggaa accaggcctt aagcgcagat aacatgaaca atgccgcagg
     6121 cattctgcaa tactgcgcga agcaaaagct ggcttcggta accgatgtcg aaaacatcaa
     6181 gaaccaggtg ctggaaaagc tgggcctgaa cagtgaagag caaaaagaag acaccaacta
     6241 tctggacggc attcagggtt tgctgaaaac caaagatggt cagcaactca atctggataa
     6301 catcggaaca actccgctgg cagaaaaggt gaaaaccaaa gcctgcgatc tggtgttaaa
     6361 gcaggggctg aacttcattt cctgaaccgc gaaacgccgc gttttagctg ttacccggct
     6421 gacgcggctc tctcctgccc ttttccctgc tcctggacgg ttttccccct gttacacggg
     6481 aagcgaccgc gatcaaaaaa gtctgtttat aaactaattc ctaacaacat cgcatttttt
     6541 tgacactaga attgcagcaa tatggcgacg ccattacatt gtgcttctca aaaagcttaa
     6601 acgttggcta aacggcccgg tttagtgagg atcgcccgtt gtcagaacta ctctctttcg
     6661 ccctttttct cgcctctgtg ctgatttacg catggaaagc gggacgtaac acctggtggt
     6721 ttgcagccac attaacggtg ctggggctat ttgtcgtttt aaatatcacc ctgtttgcca
     6781 gcgactattt tactggcgat ggtattaacg acgcggttct ctatacctta accaacagcc
     6841 tgaccggtgc tggcgtcagc aaatacattt tgccgggtat cggcattgtg ctggggctga
     6901 cagcggtgtt cggtgcgctg ggctggatcc tgcgccgtcg tcgccatcat ccgcaccatt
     6961 ttggttacag cctgctggcg ctcttactgg cgctgggttc ggtggacgcc agcccggcat
     7021 ttcgtcagat aacggaactg gtgaaatccc agtcacgcga cggcgacccg gactttgcgg
     7081 cttattataa agagccgtcg aaaactatcc ctgacccgaa actcaacctg gtttatatct
     7141 acggcgaaag tctcgagcgg acctattttg ataacgaggc tttcccggac ctcacgcctg
     7201 aactgggcgc gttgaaaaat gaaggcctgg atttcagcca cacgcagcag ctgccgggaa
     7261 cagattacac gattgcgggc atggtggctt ctcagtgcgg cataccgctg tttgccccct
     7321 ttgaaggcaa cgcctccgct tctgtttcca gcttcttccc gcagaacatc tgtctgggcg
     7381 atatcctgaa aaactcgggt tatcagaact atttcgtgca gggcgcgaat ctgcgttttg
     7441 ccggtaaaga tgtgttcctg aagtcgcatg gcttcgacca cttatacggc tcagaagagc
     7501 tgaaaagcgt ggtagccgac ccgcactatc gcaacgactg gggattctac gacgataccg
     7561 ttctcgatga agcgtggaaa aagtttgaag agctttcccg ctcaggtcag cgattctcac
     7621 tgtttaccct gacagtcgat acccatcacc cggatggttt tatctctcgt acctgtaacc
     7681 gcaaaaaata tgattttgac ggtaaaccga atcagtcatt cagcgcggta agttgcagcc
     7741 aggagaatat cgcgacgttt atcaacaaaa tcaaagcgtc accgtggttt aaagatactg
     7801 tcatcgtcgt ctcttctgac catttagcga tgaacaacac ggcgtggaaa tacctcaata
     7861 agcaggatcg caataacctg ttttttgtca ttcgtggcga caagccgcag caagagacgc
     7921 tggcagtgaa gcgtaacacg atggataacg gagcgacagt gctggacatt ctcggtggcg
     7981 ataactatct cggacttggt cgtagcagtt tatccgggca gtcaatgtcg gaaatcttcc
     8041 tcaatatcaa agagaaaaca ttggcgtgga agccggatat catccgtctg tggaaattcc
     8101 ctaaagagat gaaagagttc accatcgacc agcagaaaaa catgattgcc ttctcgggta
     8161 gccatttccg tttgccgctg ctgttgcggg tttcagacaa acgcgtggaa ccgctgccag
     8221 aaagcgaata ctcagcaccg ctgcgtttcc agctggccga tttcgctcca cgcgacaatt
     8281 tcgtctgggt tgaccgttgc tacaagatgg cacaactctg ggctccggaa ctggcacttt
     8341 ccaccgactg gtgtgtctcg caggggcaac ttggcggtca gcaaattgtt cagcatgttg
     8401 acaaaacaac atggaagagc aagacggcat ttaaagatac ggtcatcgac atggcgcgtt
     8461 acaaaggcaa tgtcgatacg ctgaagattg ttgataacga tattcgctac aaagccgaca
     8521 gtttcatctt taacgtcgcc ggtgcgccag aagaggtgaa acagtttagc gggatttccc
     8581 gtccggagtc gtggggccgc tggtccaacg cgcagctggg cgatgaagta aaaatcgagt
     8641 acaagcatcc gctgccgaag aaatttgacc tggtgattac cgccaaagca tacggcaata
     8701 acgccagccg tcctattccg gtacgcgtag gcaatgaaga acaaactctt gtgctgggca
     8761 atgaagtgac caccaccaca ctgcatttcg ataacccaac cgatgccgac acactggtaa
     8821 ttgtgccgcc ggaacctgtc tcaaccaacg aagggaatat cctcggacac tcgccgcgta
     8881 agctcgggat cggcatggtg gaaattaaag tggtagaacg tgaagggtaa ttcctgactg
     8941 atgtgtaggt cgcatagggt gcgacctaca caccttctcc ggcttacaac acattattaa
     9001 gaaacaaaaa aaacagcgcc caacacttcc ccggacgctg catggcaact ttaataatct
     9061 cgcctttcag aaagtaatta cgcctttaat taactcacga ttgttaatca catcgcgctc
     9121 gtaggtttct gccagcgtgg cgaacggata gcgatgggtt aacatcatgc cagcagtgat
     9181 ttttccttcc gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct
     9241 gcccatcatc gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt
     9301 aaacaggccg acaaatacca ccgtgccgcc gtgacgaatc agattcacgg tgttattcat
     9361 cgcatgttga ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc
     9421 ccgcagctgc gcgtcaaaat cttcggctga cgggtccagc acgggcaact ccagacgcgt
     9481 tgccacatgt tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc
     9541 tttagcgatt gccgccgcgc ccagaccgat tggcccggcc ccgaccacca gcacctgctc
     9601 gccgggagca atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa
     9661 tgcggccgcc tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa
     9721 atactcacta aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt
     9781 ggtacgcccg cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac
     9841 ttgctgacca tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat
     9901 ttcatggcct aaaacccgtg gataactaaa aaatggttga tttccacccc aggcatgaat
     9961 atcggtaccg caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat
    10021 aggtatctcg cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat
    10081 cgtagacatg attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat
    10141 ggcagcggaa cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg
    10201 ttttaatgga ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg
    10261 attaaccagg tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa
    10321 agtgcgctgg cggaaatgta caacatcagc cgcactacag tgcgtcatat tctcagccac
    10381 ttacgcgaat gcggcgtctt aacgcaggtt ggaaacgact atgttattgc gcgtaaacct
    10441 gaccatgatg acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt
    10501 gagcaggctt ttttcacgat gataaaccag cgccagttac gcccagggga aactttttct
    10561 gaactgcaac tggctcgggc cgcgggtgtc agcccggtgg ttgtaagaga atatctttta
    10621 aaattcggcc gttataatct tattcaaagc gaaaagcgcg gccagtggag catgaagcaa
    10681 ttcgatcaat cctacgctga gcagctgttt gagctacgcg aaatgctcga aacacattca
    10741 cttcagcact ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg
    10801 ctcgaacgtc atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa
    10861 cttgacagag attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa
    10921 tcgcttgaga tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt
    10981 aaacagcgca atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc
    11041 cgcagtgacc ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc gaaacaatca
    11101 atgattcgat ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc
    11161 aaactattag cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc
    11221 tatgctcaca atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt
    11281 taacctgccg ttaatgtcaa ccttgcgtaa tgcacaatta cgtaaacaat taaaattata
    11341 aatacaggag tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac
    11401 tccatcttcg tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg
    11461 aattaaacgc attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta
    11521 tctcgaccgc agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag
    11581 tgccaccgaa atcggcgctt tgctctccgt gttttcactc gcttacggca ttgcgcaact
    11641 tccttgcggc ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt
    11701 cttctggtca ctgttccagg ccatgtctgg catggtgcac agctttacgc agttcgtgtt
    11761 ggtgcgtatc ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat
    11821 taacgactgg ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc
    11881 taccattggc gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg
    11941 gcgcgggatg tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat
    12001 gctctatcgc aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc
    12061 aggtagcgtc aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg
    12121 taaccgcaca atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct
    12181 gtatctggcc tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac
    12241 agggttgatg gcggctatcc ctttcctgtt tggtgctgcc gggatgctgg tcaacggtta
    12301 cgttactgac tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat
    12361 tattgccggg atgttctgtt ctgccgcctt tacgctgata gtaccacaag cgacaacatc
    12421 catgatggcg gttctgctga ttggcatggc actgttctgt attcactttg ccggaacatc
    12481 ctgctggggc ttgatccacg tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat
    12541 ccagaacttt gccagcttca tctgcgcctc tttcgcgccg atcattactg gttttattgt
    12601 tgatacaacc cactcattcc gtctggcact aatcatttgc ggttgcgtca ccgcagcggg
    12661 agcactggcg tacatcttcc tggttcgtca gccgatcaac gacccgcgta aagattaaac
    12721 tccctatgcc cgataacatt ttgcttatcg ggcattttta tggcgattaa aacgtttccc
    12781 agttctcccc gctatctgcc actgccattt tacgcggcgt agctggcgtc acggttttta
    12841 ccacagccga tgtttcacgc tgctgttgct gctgttgctg aatccggaac actgccacag
    12901 cttcggtcag gcgactggcc tgctcttcca acgcggcggc ggcagcggca gactcttcca
    12961 ccagcgcagc gttctgttga gttacccggt ccatctcagc aaccgctaag ccaacctgat
    13021 cgataccacg gctctgctca tcagaagcag aagctatttc gcccataatg tccgtcacgc
    13081 gggtcacagc actgacaatc tccgccattg tttccccggc gctttcgacc agcgtagagc
    13141 caacatccac tttccccacc gagtcttcaa tcaggctttt aatttcacga gccgcctggg
    13201 cgctacgctg ggccagatta cgcacttctc ccgcaaccac cgcaaaacca cgtccttgct
    13261 cacccgcacg cgctgcttca accgccgcgt tcaaagccag aatattggtc tggaaggcaa
    13321 tgccgtcaat tacgctgata atatcggcga ttttctgcga actggtggag atatcacgca
    13381 tagtctgcac cacgttatct accactttgc cgccgcgttg cgccgtttca gaagcactta
    13441 acgccaggtg gctggcctgg cgcgcattct cggcgttctg tttcaccgtt gcggtgagtt
    13501 gctccatgct ggctgccgtc tcttccagcg aagcggcctg ttgctcggtg cgcgaagaga
    13561 gatcgttatt gccggtagcg atttcgctgg caccgctata gatggcattg gccccgttgc
    13621 gcacatcacc gacggtacgc atcagctctc cctgcatatg gcgcaaactc tctgccagtt
    13681 gccccatctc attagagcca tccacctcaa tcggtttcac cagatcgccg cctgcaatat
    13741 gacgaatgct gtcaatcagg cgattcattg gcgctaccag cgaggcttta ataccgaacc
    13801 agacggcgaa gatgaccgcc agtacgacga tcatcacgcc caccagaatc cacatcgcct
    13861 ggctgtagga ggcattgtta tcgctgacgg cgatatcata gagccgatcg ttttgctcca
    13921 tgtaagccac atactgcttc tcgaaaccgt cctgatatcc ctgggtcggc tgatcaaaga
    13981 actcgttgat tttgcctgca cctaacagtt ggatcagctc cgccagcgca ttgtgataaa
    14041 tatcgtaatt acgtttgatc tctgccgctg cggcggtgct ctgacgcggg tcacgcggca
    14101 acgcttcgta atctgcccag tttttttccg cctgtttcaa cgaaatactg gcactctgca
    14161 tcagctcagc aacggttgaa ccgctaccaa tattattctg atccatcatg tagcggatac
    14221 ccgcgcggtt gagggtgtta cgcgtctgca acaacgcgac ccagctgcca ttcagcgtgg
    14281 attgctgctg gcgaatggtt tgtaaaacag tgaaattttc tttgtcattc tttaaggcat
    14341 taaagaacag accgcctgat gtcagttgta aaaggccaaa aacggccaaa accagcagta
    14401 agctggtcac gatcttgata cgttttaaca tggtttctct ttcctgtgga ccgatgcaaa
    14461 attttcggcc tgaaatgaga aaactttata ggtagcgact tgaaactggt taattttacc
    14521 tcactttttt ttaatttttg t
//
LOCUS       DAZFBR010000117        14403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFBR010000117 DAZFBR010000000
VERSION     DAZFBR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.117"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            71..427
                     /locus_tag="ACHJZ2_003909"
     CDS             71..427
                     /locus_tag="ACHJZ2_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4569934.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(478..1374)
                     /gene="yhaJ"
                     /locus_tag="ACHJZ2_003910"
     CDS             complement(478..1374)
                     /gene="yhaJ"
                     /locus_tag="ACHJZ2_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO4569935.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFNGK"
     gene            1479..2180
                     /locus_tag="ACHJZ2_003911"
     CDS             1479..2180
                     /locus_tag="ACHJZ2_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO4569936.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2203..2367
                     /gene="yhaL"
                     /locus_tag="ACHJZ2_003912"
     CDS             2203..2367
                     /gene="yhaL"
                     /locus_tag="ACHJZ2_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO4569937.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2501..3811)
                     /gene="cyuA"
                     /locus_tag="ACHJZ2_003913"
     CDS             complement(2501..3811)
                     /gene="cyuA"
                     /locus_tag="ACHJZ2_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO4569938.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3839..5170)
                     /locus_tag="ACHJZ2_003914"
     CDS             complement(3839..5170)
                     /locus_tag="ACHJZ2_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4569939.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5445..6809)
                     /gene="tdcG"
                     /locus_tag="ACHJZ2_003915"
     CDS             complement(5445..6809)
                     /gene="tdcG"
                     /locus_tag="ACHJZ2_003915"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO4569940.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6881..7270)
                     /locus_tag="ACHJZ2_003916"
     CDS             complement(6881..7270)
                     /locus_tag="ACHJZ2_003916"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO4569941.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7284..9578)
                     /gene="tdcE"
                     /locus_tag="ACHJZ2_003917"
     CDS             complement(7284..9578)
                     /gene="tdcE"
                     /locus_tag="ACHJZ2_003917"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO4569942.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9612..10820)
                     /gene="tdcD"
                     /locus_tag="ACHJZ2_003918"
     CDS             complement(9612..10820)
                     /gene="tdcD"
                     /locus_tag="ACHJZ2_003918"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO4569943.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10846..12177)
                     /gene="tdcC"
                     /locus_tag="ACHJZ2_003919"
     CDS             complement(10846..12177)
                     /gene="tdcC"
                     /locus_tag="ACHJZ2_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO4569944.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12199..13188)
                     /gene="tdcB"
                     /locus_tag="ACHJZ2_003920"
     CDS             complement(12199..13188)
                     /gene="tdcB"
                     /locus_tag="ACHJZ2_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO4569945.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13287..14225)
                     /gene="tdcA"
                     /locus_tag="ACHJZ2_003921"
     CDS             complement(13287..14225)
                     /gene="tdcA"
                     /locus_tag="ACHJZ2_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO4569946.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3729 a         3854 c         3360 g         3460 t
ORIGIN      
        1 aaagatacga atagcattta cacaataccc ggaatagact ttcgtcactt ttaattaaag
       61 ggatgttttt atgcagtggt atttatccgt attaaaaaat tatgttggtt tctctggtcg
      121 cgcacgtcgt aaagagtact ggatgtttac tctgattaac gccatcgtcg gcgctattat
      181 caatgtcatt caattgattt taggtctgga gcttccatat ctgtctatgc tttatttgct
      241 ggcaaccttc cttcccgtcc ttgcgcttgc tatccgtcgc ttacacgata ccgaccgttc
      301 aggtgcctgg gccttgctct tttttgttcc gtttattggc tggctcgtgc ttctggtttt
      361 tttctgcaca gaaggtactt ctggcagcaa tcgttacgga aacgatccga agtttggttc
      421 aaattaatct tagaattggg gcgatatttc gccccttttt attaacaata ataaatatta
      481 ttttccgtta aaaagtttgg gaatttcccg cagacaccag gatttcgctt cccccatact
      541 gtcacgacgc caggccataa taatatcgat ctcgctggtc gattccgggc tgacgacacg
      601 caaccgccct tccgcaatat ctttttcgac catcggatac ggcatcgtcg ccacgccaag
      661 ccccgccagt aatgcctgac gtttatcttc aatcgtgctc accgttaagc gcggctgttt
      721 gtccagcagc tgtacagtca acaccgggcg ctcacgagcg gtatccgcca ccgcaattcc
      781 acgatatttc acacgcgtca cttcagataa cggttccggc tcctgatgaa tcgggtgatc
      841 aggcgcggca acgtagacgt tcattaacgt atagagcttg cgcgagttga tctccgacga
      901 ggaacgaaaa tgcatatccg gcgcgataac aatatccgcc cgcccctgct ccagccgttc
      961 ccacgccccc gccagcactt ctgtgatgat tgccagttgg gtattggctt ttgccgccag
     1021 tttgtcgatt aacgggaaaa aggcaggtgt cggtaccagc gcttcggtca caatggtgag
     1081 atgcgtttcc caaccgcgcg cgagagcttc cgcatcagta gtcagtttat ctgcggcttc
     1141 cagcaaaacg cgccctcgct ccaacaacat cctcccgaca ttggtgaatt tggtgcgatg
     1201 gcccgagcgg tcaaacagca ccacatccag ctcttcttcc agtttttgca tggtgtagct
     1261 aagtgcggaa ggcacgcgtc ccagctcatc cgctgccgcc gcaaaactgc cccggcgatc
     1321 gatcgcatcc ataacccgta gtgcttccag cgttaatgcc ctttctttgg ccatttcgtt
     1381 ctcattcagg aaatttgaac ataccgggca gaatatctgg ctaacaatgt agcgtccagc
     1441 cccttaccat aaaaggaagt aaagagaggt caagaattat gattactacc cgaactgcca
     1501 ggcagtgtgg acaagcagac tacggatggt tgcaggcccg gtatactttt tcctttggac
     1561 actacttcga cccgaaattg ttaggctatg cctccctgcg tgtgcttaac caggaagtgc
     1621 tggccccagg tgccgccttt cagccgcgaa cctatcccaa agtcgatatt ttaaatgtga
     1681 ttctggatgg agaagcagag tatcgcgaca gcgaaggcaa tcatgttcag gccagcgccg
     1741 gtgaggcgtt gctgctctct acccagccgg gtgtcagcta cagcgaacac aatctcagca
     1801 aagacaaacc gttaacacga atgcagcttt ggctggacgc ctgcccgcag cgagagaatc
     1861 cgctgattca aaaactggcg cttaatatgg gcaagcagca tttaatcgcc tcgccagagg
     1921 gtgcgatggg aagcctgcaa ttacgccagc aagtgtggct gcaccatatc gtgctcgaca
     1981 aaggcgaaag tgcgaatttc cagttgcatg ggccacgcac gtatttgcaa tcgattcacg
     2041 ggaaatttca tgcgcttacg catcatgaag agaaagcggc gctgacctgc ggtgatgggg
     2101 cgtttattcg tgacgaggct aacattacgc tggttgccga ttccccactg cgcgctttgc
     2161 tgatagattt gcctgtctag ttgtttttac agggagatga tgatgagtaa aaaatcggcc
     2221 aaaaagcgcc agccagtgaa gcccgtggtg gcgaaagaac ctgctcgcac cgccaaaaat
     2281 tttggctatg aagagatgtt gagcgagctg gaagccatcg tcgcggatgc tgaaacgcgt
     2341 ttagccgagg atgaagctac cgcgtaaaga caggttgccg gatgcggcga aaacgcctta
     2401 tccggcctac aaaatccaac aacattaacg aattaaacaa cccgtaggcc ggataagatg
     2461 cgccagcatc gcatccggca cgatacccaa aacctggcgt ttatctggcc ttgctcgcca
     2521 taatctcgat aatctgccga tccgtttgct gcatcgaatg gcttgctaac gcacacaggt
     2581 tggcaatcga ctgctcaaca tcatgcgcca cgatcccttc attgccggtc acggcggtat
     2641 catccagcgc cattaacacc gctttccacg cagccgaagc actggtcgaa accttcatcg
     2701 cgcagctgtt cgacgcacca tcgcaaatca tgccgctgac atcgccgatc atactgctga
     2761 tcgccatcga gatggtttca taacgcccat ccaccagcca tgccatcccg gcggcggccc
     2821 ccattgctgc ggtcgttgcg gcacacagcg cagacaaacg cggtaactgg ttatggatgt
     2881 aaattgcgct caaatgagaa agcatcagcg cacgcgccag ccgttcatca tccgctccga
     2941 agtgttctgc taccaccacc acgggcattg ttgcggtgat cccctggtta cccgagccgg
     3001 agttactcat tgccggaagc gtggcgccgc ccatacgcgc atcggatgcc gcgctggtac
     3061 gaatcacaat ggatgaagag agatctttcg ccagcaaacc gcgctcgcac tgtttttcca
     3121 gcgtcgcgcc aatatgcagc ccccacttac cgctcaaacc ttcctgcgat aacgcacaat
     3181 ttagcttcgc ggaatcgaga ataaagcgga tcgccgcaaa cgggacttca ttgacgaact
     3241 tcaggatctc agccagcgtc gttctggaaa gcaccgatag cggagactct tgctcgccct
     3301 ctgccacaca cgcctgctgg gtaaacacca caccatcgtg cgtctcaata tgcacaatgt
     3361 tggtatgccc gccgacgata gtgacacacg cccacttctc accgttccag actttggcgc
     3421 gtgagaagag gatttcattg caaggttcct ggatcttaac ggagactttc cccgccgcca
     3481 gcagtgcttt ggcatcggca attgcctgcg cagttgcgtc tttcagcact tccagcccgg
     3541 cgttggcatt tccacctaac gcccccagcg ccgccgcaat cggcagcccc accattcccg
     3601 tgccgggaac ggtgacgccc agaccgttct tcatcagatt tggcgaaacc caggcttcta
     3661 cacgttcaac cggaccttcc agttctgccg cagcaaccgc cgccgccagc gccagtgaaa
     3721 tcggttcagt acatcccagc gccggtttta cttcctcctg aacggcgagg atgtaacgct
     3781 gccataacgg atttaaagtc gaatcaaaca tattaaaaac cttaaaattt caggtaaatc
     3841 aagaaaatgc caggaacgga gaaacacaaa gcaacaaacc agtgacgata atcaggtaca
     3901 gagacatccc tttgtatttg tgcaatgccg gtactttgta aaccagccac gccgggatca
     3961 ggcaccctac caagccgaaa atcgggctac agatagaggt gaagctcaac accggtgcgt
     4021 tcagtacgat ggcgctccag gccagcaaaa tggcgaaaat catgatgccg cgctgaacaa
     4081 gattttcgtt aatcttctcg gcaggcatct tgcgacgcag gatgttcatt acgatccctt
     4141 gcgttgcttc gcgaaaacct aaatagacgc caaagaatgc agtcatgaca gcaaagatat
     4201 tgagaatgac gctgaccact ttcacccacg ctgcgccgtc accgctaata aactgtgcgg
     4261 caatcgccag cgcggaaata ttctgctcgt aggcttttac cgcttcgtca tgtcccatcg
     4321 ccagcgtgaa cgacacggcg tagaaaaaga cgatgataaa caaaatgcca aacgcgatat
     4381 tcattgcccg caatgcttta tgccgcgcta cttcaatcga tttttcccgc gagcgataag
     4441 agatcaccat cgggcttaac gtctggataa acagaatcga cgtcagggta aacggcagcg
     4501 taataatagc gtttttcacc agcagcccca gcggcggtag cgaaccgacg ttatacagat
     4561 gccacattcc caccatcgac acgcccagcg ccgcgaccac cagcagcttg gtcagcacca
     4621 tgccagtcga aattttgaat aacaatttct cgccgcgtga agagatcgcc accagaatgc
     4681 aaatcagtac cagaccataa aagggactgt ctgacagcaa cccttccgtc acgccgaagg
     4741 tatgcaggta ggaagcacta tcgttggtga tggcggtgga atagacgaac atccaaatca
     4801 ccagcattac gaaatagagt gcacctaaca ggatgcccca gtttttacct aaataaccgc
     4861 taatgacgct cgggtaatct ttacattctg gtgattctgc cagcgtatta ataaacaacc
     4921 gctgaaacag atacattgcc gggtaaccaa tcaccgatga gagcaaaaat acccacaatc
     4981 ccatcaaacc gacctgcacc gggagaaaaa caatccccgc gccaatcgcc atcccgatac
     5041 tcataatcac ccagccggtg tcagtactgt cgaatttgat cgcttctcgc cactcgctct
     5101 cactcattcc agcacgaccc gccggggtcg aagcgtctgc aatgacgcct ttattcgatg
     5161 caatttccat aatttctcgc tcaatatttt gtagggctta ttattttttt ccgagccgca
     5221 tcaaggcgat atgcggtcta cgtttttgca ggcgagttat tagaataaag aaatgatacg
     5281 cgcgaggtca gagaaaatct tcacaatcaa gactttgaaa taacggagat ggataagaat
     5341 tttctactaa attaatcgca agagaatatt cactctctat ttgcggcaga tcacaaaaaa
     5401 aaggtgcaca tttgtgcacc caaggatgaa agctgacagc aatgtcagcc gcagaccact
     5461 ttaatggcca gtccgccgcg tgatgtttcg cggtatttat cgttcatatc tttgccggtt
     5521 tcatacatcg tctcgatcac tttatcgagt gaaacacgcg gtgccgaggt gcggcgcatc
     5581 gccatccgcg cggcgtttac tgctttcacg gcattaatgg cattacgttc aatgcacggg
     5641 atttgtacct gtccggcaac cggatcgcag gtcagtccaa ggttatgctc catcgcgatt
     5701 tccgccgcat tgcatacctg cgccggactg ccgcccagta gttcagttaa ccctgccgcc
     5761 gccattgaac aggccacgcc aatctccccc tgacagccga cctccgcgcc agagatggag
     5821 gcgttcattt tatacagcgc gccaatagcc ccggctgcaa gaaaatagcg ggcaattgac
     5881 cgctcgttta ccggacgacg gaacttatcg taataagcca gcactgccgg aataatgccg
     5941 cacgcaccgt tagtcggtgc cgttaccacg cgcccgccag ctgcgttttc ttcactaacc
     6001 gccagcgcgt acatgttgat ccagtcgatg acattcatcg gatcgttaga gatgttatcg
     6061 ctggaaacca gctgacgacg cagcgctacg gcacggcgcg gcacattgag cggaccaggc
     6121 agcacgcctt cagtgttcat cccgcgttca ataccgtcat gcatcacttg ccagatacgg
     6181 gcaaaaccgg cgtcaatttc cgctttactg cgcagcgcta gctcgttgtg catcatcagg
     6241 ccagaaatag acaggccgtt gtaatcacac attttcagca gttcacctgc tgagtggaaa
     6301 tcgtaaggta cgggtgtttc gacatcgtgc gacaggccga agtgttcttc ttcgacaata
     6361 aacccgccgc cgacagagta ataggtttta cttaatagct cttcctgtgc cttccaggca
     6421 gtgatccgca ttccgttctc atggcgaggc aacatttcgg gatggaagat aatgttcttt
     6481 gctacaggaa aatcaacaat atgcgcacca gatgccactg gcagccgccc gctgcgcgtt
     6541 actaactcta taaatgcagg gatctcatca atgacaacat cctgcggact gtttcctgcc
     6601 agtcccatga tgatggcgac atccgtggca tggcctttgc ccgtcagtga caacgacccg
     6661 tacagatcga ccacaatatg gctcgtcgcg gttaataagc cgctactttc cagccgatca
     6721 ataaaacttt ttccggcatt cattggcccc acggtatgcg aactggaggg accaatccca
     6781 attttgaaaa tatcgaatgc actaatcata tccacaccct cggattgccg ttcagtgaag
     6841 tggagcggaa cgaccttacg accgtcccgc tcacgaggct ttacgcacta cgtactgcga
     6901 tagcttcaat ttccagcttc acatctttcg gcaaacgcgc gacctgtaca cagctccggg
     6961 tcgggtaggt cgcctgatgc tcatcgaaga attgcttata gacttcgttg atggtggcaa
     7021 aatcattcag atcggtgatg aacacggtca tcttgatgat atcgcccacg ctcagcccgg
     7081 cagcaaccac gatcgctttg acgttttcga ggcttaaacg cgcctgatct tgcacatcag
     7141 ccgggatctc accggtctgt gggcaaaccg gaatttgacc ggaggtgaag accatgctgc
     7201 ctaaatcaac gccttgaaca taagggccga ttgcgcctgg ggcacgttgc gtttcgataa
     7261 tctttttcat acatcctccg gcgtcagagc gcctgggtaa aggtacgtga aataacatcc
     7321 tgctgctgtt cacgggtcag tgcgttgaag cgcacggcgt agccagagac acggattgtc
     7381 aggttaggat atttttccgg gtgctcgatg gcatccagca gcatttcccg attcattacg
     7441 ttgacgttga ggtgttgacc gccttcgaca tccgcttcgt ggtggaaata cccatccagc
     7501 aggccgacaa ggttggtttt acgtactgga tcttctttgc ccagcgccgc agggacgatt
     7561 gagaaggtgt acgagatccc atctttggcg taggtgaacg gcagtttcgc caccgacgtc
     7621 aatgaggcca cggcaccttt gcggtcacga ccatgcatcg ggttagcgcc cggcgcgaac
     7681 ggtgttccgg cgcgacgacc gtccggcgtg ttaccggttt tctggccgta caccacgtta
     7741 gaagtgatag tcagaatcga ctgggtaggg acggcgttgc gataggttgg cagcgcttta
     7801 attttcttca taaaacgttc aaccaggtcg caggcaatgc tgtctacgcg ctcgtcgttg
     7861 ttgccgtact gcggatattc accgtcgatt tcaaagtcca ccgccaggcc gtttttgtca
     7921 cggattggtt tcacgcgggc atatttgatc gcagacaggg agtccgttgc caccgacagg
     7981 cccgcgatgc cgcacgccat ggtgcgatag acatcacgat cgtgcagcgc catcagcgaa
     8041 gcttcgtagc tgtacttgtc gtgcatgtag tgaatgatat tcagcgcgct gatgtactgc
     8101 accgccagcc agtccatgaa gtgatcgagg ctgtccatca ctttgtcgta atccagcaca
     8161 tcgtccatca acggtgccgt tttcggcccg acctgaatct tcagcttctc gtccaccccg
     8221 ccgttaattg cgtacagcag tgttttcgcc aggttagcgc gtgcaccaaa gaactgcatt
     8281 tgcttaccaa tcaccattgg gctgacgcag caggcaatcg cgtaatcgtc gctgttgaag
     8341 tcagtacgca tcagatcgtc gttttcatac tgcaaggaag aggtgacgat cgacacctgc
     8401 gcggcatatt ttttgaaggc aatcggtaat tcttccgacc aaagaatggt caggttaggt
     8461 tccggtgcag gccccatagt gtgcagggtg tgcaaatagc ggaaggagtt tttggtcacc
     8521 agcgtacgac cgtccagccc catcccgccg atcacttccg tcgcccagat tgggtcgccg
     8581 gagaacagcg aatcaaattc cggcgtacgc aagaagcgca ccatacggat cttcatgatg
     8641 aagtgatcga tcagttcctg tgcctgctgc tcattgagta cgccagcttt aaagtcgcgc
     8701 tcaatgtaga tatccaggaa cgatgccgtg cggcccagcg acatcgcgcc gccgttctgt
     8761 gatttcactg ccgccagata agcgaagtag agccactgca ccgcttcctg cgcattctgc
     8821 gccgggcgag agatatcaaa gccatatttc gccgccattt cctgaatctg caacagcgca
     8881 tgacgatgct ctgccagctc ctcacgcaga cggatggtgg cttccagatc ctcgcctttt
     8941 tccagacgag actggagatc ggcaaattgc agttcgcgtt cacgcaccag ataactgatg
     9001 ccatacagcg ctacgcggcg atagtcaccg ataatgcgcc cacggccata gccatctggt
     9061 aaaccggtca gcacgccaga tttacggcag cgcagcatat ccggtgagta aacatcaaat
     9121 acgccctggt tatgggtttt acgcagatcg gtaaacagat attcaaattc actgtccatt
     9181 tcacgaccat aggcgtggaa tgaactttta atcatattaa tgccaccgaa cgggtgtaac
     9241 gcacgtttca acggcgcatc cgtttgcagg ccaacaattt tttccagcgg ctggttaata
     9301 tatcccgcat catgagcggt aattgtggtg gcaatattgg tatcgaaatc aaccggcgcg
     9361 tgggttgcgt tttcaatacg aatgccttcc atcacctttt cccacaattc cgtggtggct
     9421 ggcgtcgctt cagcgaggaa agattcatcg ccttcatacg gtgtatagtt atgttgaata
     9481 aaatcgcgga cattaatttc gtttttccag tccgtacctt taaagccaag ccatgcgtcg
     9541 gcgtacagct tatcgctggt atcaatatct accttcatga aaaataatct ctctacaata
     9601 cttcaactaa attatgcaaa ttctgcgggt gcgttaactt tgcctaaatg aatggcatcc
     9661 aaagcaatca ttttttcttc gttggtcgga attacggcgc aaatgacacg cgcattttca
     9721 ctggaaacaa ttcgctcgcc aaaagagtta gatcgattat tcatttctgt atcaatctct
     9781 acgcctaata cagccaaatg ttccatgacc agacgacgaa ttaaacttga attctctcct
     9841 attccgccgg tgaatataat tccatccagg cgacgtaatg aagctgcgtg tccggcaata
     9901 tgacgggcaa ttcggtgaac aaaggtttta attgccagct gcgcgcgttc gtgaccttca
     9961 tgccaggctt tttccagaac acgtaaatcc gaagaaagac cggaaatacc taataatccc
    10021 gactctttat tcactacgcg ttccaggtca cccaggctct ggttggtttg gctggcgacc
    10081 caggacatcg caccaaagtc gacatcgcca ctgcgggtac ccatcatcaa gccttccagc
    10141 ggcgtcattc ccattgaggt gtcaacactc tgaccgttgc gaactgcgca gattgacgcg
    10201 ccattgccaa gatgcgccac aaccaggccg gagtcatctt ccgccagatt cagcaaagaa
    10261 tgtgcgcgca gggaaacata gcggtgcgac gtgccgtgga aaccataacg gcgtacacct
    10321 aactcttcat aatatttcca cggcaggccg tataaataag cttccggagc catcgtctgg
    10381 tggaaactgg tatcaaatac cgccacctga gttacgcccg gaaataattg ctgcgccgat
    10441 tcaataccac ttaaattggc gtaattatgc aggggtgcca gtggagaaac gcgacggata
    10501 ttatcaatga cttcatcggt aataatggcg gactcggtaa aaatactgcc gccgtgagcg
    10561 atgcggtggc caattaaggc cacgctgtca tttaaattcc gtttttccag ttcaaatgca
    10621 attgccttca atgcaccttc gtagctgtgg tgagccagcg gtgctggctc tcccccattt
    10681 acggataaga atgcattttc cgagttaata ccgtcggcaa tacctgacat taatacttca
    10741 cagtcgctgg catcgagtac ggaaaactta atcgaagacg aaccacagtt aataaccaaa
    10801 acaaccggaa attcattcat ctcttttctc atcctgagtt acggattaaa acagtttata
    10861 tacgatgttc aggatggtca gcagaccaat cacggtaaca aacacgttat ccagacgacc
    10921 acggtatttc gccagagacg gcgctttacg gatggcatac atcggcaaca ggcacagcag
    10981 ggatgcgata atcggtgcgc ccatggcttc aatcaggtcg aggatgttcg ggttggcgta
    11041 ggcaacaacc caggtggagc ccatgatgaa gatcatgctg atagtgttca gtttacccag
    11101 cgatacttta gttttgtcgc ctttataacc aaacttcagg accaggccat tcagaccttc
    11161 cagcgttccc agatagtgac cgaagaaaga tttgaagata gccacgagtg cgatgatgga
    11221 agccgcatat tccagtgtaa tcgcgaacgt tgttttggta ccggtcatgg acgcaaagtg
    11281 gttagccaga taagaaagca ctggaatatt ctgcgctttg gcttccgcca tgttggccgg
    11341 agacagcgta aacaggcagc taaaggcaaa gaacatcacc actgcaacca tcagcatgct
    11401 ggcacgagaa atgatttggg aacatttacg ttcggtgaag tcgcgaccga agtctttctc
    11461 atactcttca cgtttagaaa ccacgaagga agagacgatt ggcgagaagt taaaggagaa
    11521 aaccatgatg gaaatcccca gccagacagt gatcaggata ccgtcatgac cggttaacga
    11581 cagcgaaccg aggtcaacct ggtcgataac tgcagagttc cagtaaggga tcagcgacaa
    11641 agaaatcagc accaggctgg cgataaacgg ccataccagg tagctcatca ctttaaccat
    11701 cagatcctta ccaaaccaga tgacgaaagc catcagcagc aacaggaaca gcgccacaaa
    11761 gccgcgattc agcggtgcaa agccgagctg gttttcccag aacgtcataa aggtattggt
    11821 aatagtaacg ccataaatcc acagcagtgg gcaaatcgcg aagaagtaca ggaacgtgat
    11881 aaccacgccg ccagttttac caaaatgctc ttccaccgtt tccgtaatgt tgccggaagg
    11941 gttagagcca gaaagacaca gacgcgccag cgcccggtgg caataaaatg cgatggggta
    12001 tgccaatacc aacatcagaa gaatcgggat cagtccgcca aaacctgcgc ggatagggaa
    12061 gaacagcacc ccggcgccga ttgccgtacc aaacaagcct aacgtccatg tggtatctga
    12121 tttacgccag gacgattgtt ttgtctggct ggatacaatg ctatctgaag tactcatatc
    12181 ctatcctcaa cgaattaatt aagcgtcaac gaaaccggtg atttgagaga cgcgagaaag
    12241 atcgatattg ccgccggaaa taatactgac ggtttttctg ttttgaatat attggtctaa
    12301 tttaccgctt aataatgcag cacatgccag agcgcctgcg ccttcggtga cgactttatt
    12361 gcgctgaatt aaggcaatca tactgtttct gatttcgtct tcgctgacca gcacgatgtc
    12421 atcgactaat tcacgaacga tttcgtaagt taaattaccc gggcgggaga catcacaacc
    12481 atccgccagg gtgccggtag ttcggtgcgt ggttatttct ccggagtgga aagaagccgc
    12541 catgccgtga acgttttcag actgtacgcc aataacacga atggtcgggt taatagattt
    12601 aattgccacc gcaataccag caattaaacc gccaccacca attggcacaa tcacgttatc
    12661 gacatcatag agatcttcca taatttccag gccaatcgtt ccctgaccag caatcacttt
    12721 cggatcatcg taaggtggga taaaaatacg gccttccatt tcgacaattt cgctcacttt
    12781 agcgatagtg tcgttgaagt tatcaccatg cagaacgact tctgcggagt agtcgcacgt
    12841 tgccgctact ttggatttcg gcgcgccttt tggcatcacc actttaccgt cgatacccag
    12901 catcgcgcag gagagcgaaa ccccttgcgc atggttgccc gcagaacagg ccaccacgcc
    12961 tttgcgtttt tccgcatcag tcagtgaact taatttatta aatgcgccac gaattttaaa
    13021 tgaacccgta cgctgcatat tttcaaactt caggaatatt tcacctttgc aacgttcact
    13081 aaaatagttg gagcgaggca tgcctgtttt ataaattcgc ccagccagtc gttgtttcgc
    13141 ttcaataatg tcatcaatag caaccggcag atcgtatgta atatgcatta taaaacctct
    13201 tcgcctgaat taaatatgta ggtaaccgta accgacacct gcaagacaga caggtggatt
    13261 atttatgttt aaataaaaac aaataactaa ccaacttcta ttaattgcct tcgtctacac
    13321 ccattataag atgaatactc tttggctaat tccaccaaaa ccgatgctgc ttttttaata
    13381 cgataatttt tcgaccatac cgcggcatat tgtgccacag gtaatgtttc ttcaaccgga
    13441 atagtaataa attgattaga accaaaagga gacgtcatat cacaaggaat tacagttaag
    13501 aaatcagcat tgagaacaag attataaatt gtcacgactg agtcggtttt aacgatgttt
    13561 tcaatactga tgccatttct ttgtaacgta gtaagcagtt cgctgtagta ccccatatta
    13621 gtttgtggca acacccactg ttcgttcttc aacgactcca gcgtggtggt gccggtgcat
    13681 gttcgggact tactggctac cagcacaaac tcggactcga acagcggctc aacatgtaaa
    13741 tcctgaagct tcatttctgc gcttaacgta ccaatcgcaa aatcaagtcg accgtcgcgg
    13801 attgccggta agaacgaaga cagttgcgct tcatacatag aaacctgcgc tttcgggaac
    13861 acctctttga acttgttgat catccctgac ataaaagtaa aaccaatcaa tgaaggaaaa
    13921 ccaaatgaga cttccaccac cgcctcagaa gacataccgc ttatctcatt aaccatattt
    13981 ttcatttcac gggtaatgga ttcggaacga gagagtaaca attgaccggc aggtgttaat
    14041 gttactccgg tatttttccg caccactaat tccacaccaa aataatcttc aatatcgtta
    14101 atgattttac tgacggccgg ttgagttaac cctaattctt ttgcagccga gccgatagaa
    14161 ccacttctaa tgacttcctg aaagactacc aggtgctgcg ttttcggaag aagaatagtg
    14221 ctcataacga cctacgttaa ttacctcatt gacggcatga agtgtatcaa aatgaaatga
    14281 acaggatatg tgcgaccact cacaaattaa ctttcaatac tttccagggt atcgtcatgt
    14341 caattaaata tctttaaata tcaataaatt aaagtataaa aatcagcata aaccctgatt
    14401 ttt
//
LOCUS       DAZFBR010000118        14322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFBR010000118 DAZFBR010000000
VERSION     DAZFBR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.118"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..268
                     /locus_tag="ACHJZ2_003922"
     CDS             <1..268
                     /locus_tag="ACHJZ2_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569947.1"
                     /translation="QYKLTKNDYLKKVPLTAQLIAKYKMDDQLDQLLVTREIQDEIKS
                     KIQDKIDELSKNLFNTMTETIENNFDDIFRQQSENMSNYYEFVD"
     gene            complement(446..961)
                     /gene="pgpA"
                     /locus_tag="ACHJZ2_003923"
     CDS             complement(446..961)
                     /gene="pgpA"
                     /locus_tag="ACHJZ2_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO4569948.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(939..1916)
                     /gene="thiL"
                     /locus_tag="ACHJZ2_003924"
     CDS             complement(939..1916)
                     /gene="thiL"
                     /locus_tag="ACHJZ2_003924"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO4569949.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1994..2413)
                     /gene="nusB"
                     /locus_tag="ACHJZ2_003925"
     CDS             complement(1994..2413)
                     /gene="nusB"
                     /locus_tag="ACHJZ2_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO4569950.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2433..2903)
                     /gene="ribE"
                     /locus_tag="ACHJZ2_003926"
     CDS             complement(2433..2903)
                     /gene="ribE"
                     /locus_tag="ACHJZ2_003926"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO4569951.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2992..4095)
                     /gene="ribD"
                     /locus_tag="ACHJZ2_003927"
     CDS             complement(2992..4095)
                     /gene="ribD"
                     /locus_tag="ACHJZ2_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO4569952.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(4099..4548)
                     /gene="nrdR"
                     /locus_tag="ACHJZ2_003928"
     CDS             complement(4099..4548)
                     /gene="nrdR"
                     /locus_tag="ACHJZ2_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO4569953.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4699..5238
                     /locus_tag="ACHJZ2_003929"
     CDS             4699..5238
                     /locus_tag="ACHJZ2_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO4569954.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5537..6421
                     /gene="tsx"
                     /locus_tag="ACHJZ2_003930"
     CDS             5537..6421
                     /gene="tsx"
                     /locus_tag="ACHJZ2_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO4569955.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6598..6945)
                     /gene="yajD"
                     /locus_tag="ACHJZ2_003931"
     CDS             complement(6598..6945)
                     /gene="yajD"
                     /locus_tag="ACHJZ2_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO4569956.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(7033..7314)
                     /locus_tag="ACHJZ2_003932"
     CDS             complement(7033..7314)
                     /locus_tag="ACHJZ2_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4569957.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(7307..7549)
                     /locus_tag="ACHJZ2_003933"
     CDS             complement(7307..7549)
                     /locus_tag="ACHJZ2_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO4569958.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(7701..8672)
                     /gene="secF"
                     /locus_tag="ACHJZ2_003934"
     CDS             complement(7701..8672)
                     /gene="secF"
                     /locus_tag="ACHJZ2_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO4569959.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(8683..10530)
                     /gene="secD"
                     /locus_tag="ACHJZ2_003935"
     CDS             complement(8683..10530)
                     /gene="secD"
                     /locus_tag="ACHJZ2_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO4569960.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(10558..10890)
                     /gene="yajC"
                     /locus_tag="ACHJZ2_003936"
     CDS             complement(10558..10890)
                     /gene="yajC"
                     /locus_tag="ACHJZ2_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO4569961.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10913..12040)
                     /gene="tgt"
                     /locus_tag="ACHJZ2_003937"
     CDS             complement(10913..12040)
                     /gene="tgt"
                     /locus_tag="ACHJZ2_003937"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO4569962.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(12095..13165)
                     /gene="queA"
                     /locus_tag="ACHJZ2_003938"
     CDS             complement(12095..13165)
                     /gene="queA"
                     /locus_tag="ACHJZ2_003938"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO4569963.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            13258..13839
                     /gene="acpH"
                     /locus_tag="ACHJZ2_003939"
     CDS             13258..13839
                     /gene="acpH"
                     /locus_tag="ACHJZ2_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO4569964.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13860..>14322)
                     /locus_tag="ACHJZ2_003940"
     CDS             complement(13860..>14322)
                     /locus_tag="ACHJZ2_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569965.1"
                     /translation="EINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQGLHNVARNG
                     SPRYAGLLRQIASKSAILQDFDQQLFWRTFTGGVLHRHISVKSEPEKTDVWRFSSQFD
                     FPHINENGELSRTVLMFEGVAESNFKDIANHSSECFSYRCYAAPLDANAKK"
BASE COUNT         3605 a         3825 c         3422 g         3470 t
ORIGIN      
        1 ccagtataaa ctaacaaaaa atgattattt gaagaaagta cctttaactg cacaattaat
       61 tgcaaaatac aaaatggatg atcaattgga ccaacttttg gttacccgtg aaattcaaga
      121 tgaaataaaa tcaaagattc aagacaaaat agatgaactt tctaaaaatt tattcaacac
      181 tatgaccgaa accattgaga ataattttga tgatattttt cgtcaacagt cagagaatat
      241 gagcaattat tatgagttcg tcgactgacg atatttcagt gtcattgaat tatgagtgac
      301 aaagatacac cttgaaatat cgccgcatag tatattcggc gatgtcgggt tttactttaa
      361 cagagtgccc caaaattggg gcactgttca tttataaatc aaaccgggtg gtctggaatc
      421 gcctacacct cccggtcaat ggcatttaaa gaatacccag cggccagtga tgaccgataa
      481 agtacaggat gcctgcggaa atcaccccgg cgacaatatc atcgatcatg atccccatgc
      541 cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac
      601 ggaaaatcac aaacccggcg gcgacccact gccagtcatt ggtcggcagc gccatcaggg
      661 tgatccacat gccaataaac tcatcccata cgatgctacc gtgatcgtgc acccccatgt
      721 ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg
      781 agtagagctg ccagggcaaa aaggtcatca gataccagaa cggaatcgcc gccagcgatc
      841 ccatcgtgcc aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc
      901 acggattact catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca
      961 tatcctttcc agtctaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata
     1021 tcggcggtca tttgcccgat acaggtaaac ggtacgccca gatgaccgag agccacatcc
     1081 agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag
     1141 agcgcccagc gcagcgcctg ttccggtaca acatggcgag aaagcgcatc agaaaacggc
     1201 agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg
     1261 gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc
     1321 tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtccgc atctttagca
     1381 tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta
     1441 ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc
     1501 ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca
     1561 atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc
     1621 cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc
     1681 atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg
     1741 atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc cagggtctgt
     1801 ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac atcaagacga
     1861 gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt
     1921 tatgcctcag caggtaaaaa agaaaaggcc ggcgatcgcg gaacagtctt ccgtgaatct
     1981 accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc
     2041 cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt
     2101 taatggccac tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca
     2161 gtactgcttt ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca
     2221 gtccgtcgag gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga
     2281 cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt
     2341 tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag
     2401 cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta
     2461 atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca
     2521 cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg
     2581 ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaaatgg
     2641 gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta
     2701 gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg
     2761 ttttcatctt ttacctgacc gatacgtttc agtgcatcaa ttgcaccttc cagcaggctg
     2821 tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca
     2881 acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg
     2941 gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc
     3001 cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg
     3061 ggcgtcagct aatttctcaa gccctggcag tgtgcataat ccacgggcgt cgctgcctaa
     3121 tagtttaggt gcgatataga caatcagctc atcgactaac cccgcctgca gcaatgcgcc
     3181 agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac ccagttgcat
     3241 catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt
     3301 ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg
     3361 cacaatgtga tgttccggcg tcacgcgatt ttggctatca atcacaatac gtaccggctg
     3421 gcggagatga tgttgcgggt agagcgcctg agtttgtaca tccagttcag accaacgcac
     3481 cgttaaggca ggatcatccg ccagcaccgt agcgctgctg gttaaaatgg catgactttg
     3541 tgcgcgcagt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc
     3601 gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa
     3661 gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag
     3721 gccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg
     3781 cgggttcggg tcctgcatcg cagcgaccac gcgcgctacg ccagcggcga tgagtgcgtc
     3841 acagcacggt ggcgtacgac catgatggct acagggttcg agtgtgacat aggcggtcgc
     3901 acctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc
     3961 tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt
     4021 cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta
     4081 atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat
     4141 atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt
     4201 caattgctcc atcaccagat tgccaatcat cttgctcggc acttcgcgct caccggtggc
     4261 gcgcagctgc gatttaatat gattgattgc catttcgacg tcatcggaac tcaccggacg
     4321 tttttccagc gcccgcagca ttccgctacg taatttttct tcattaaacg gttcacgcac
     4381 gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg
     4441 ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg
     4501 agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga
     4561 ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc
     4621 ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg
     4681 acgagaaagg gacgacctat gaatattaac gtttttcgac tgatcctgct gggaagccta
     4741 ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc
     4801 acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac
     4861 aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca
     4921 agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca
     4981 gatggcacca ccctgacact acgtattcag ggtgagtcga atgatccctt gcccgcattc
     5041 agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat
     5101 gtacagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt
     5161 ccgttacagt taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt
     5221 caacctgtta tgcagtaaac ttatagcggg gcgattttgt gcgtcccgca acatccttcc
     5281 ccatcatttt gttactccgc ttacataacc cagattgata gtaaaagttt gcaacaaggg
     5341 cgaaagtcag tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa
     5401 atgatagaac tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct
     5461 ctgtatacga aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc
     5521 aaacagtggc atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc
     5581 ttcgtctttt actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg
     5641 gcaccagagc gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa
     5701 cgatacctac cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta
     5761 tgcggatgcg ccggtattct tcggcggtaa ctccgatgca aaaggtatct ggaaccacgg
     5821 ttctcctctg tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga
     5881 ccttagcttc ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg
     5941 tcgtaataaa gatggtcgcc agagcacctg gtacatgggt ctgggtaccg atatcgacac
     6001 tggcctgccg atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatgg
     6061 cgcagcgaac gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat
     6121 taccgatctg tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc
     6181 cgatttaggg gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc
     6241 tatcgcttcc agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc
     6301 tcgttactgg cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg
     6361 caacttcaac gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg
     6421 attatgaaaa tgccgggatt tattcccggc atttcagatt gttaacaacg tacgctttgt
     6481 tcatgccgga tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata
     6541 ttgctgaaat aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca
     6601 cttcttcttg ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac
     6661 atctttctgc gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata
     6721 tttcgaatgc tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc
     6781 cgggttattg gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata
     6841 aacaaactcg cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg
     6901 atagccgctt tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga
     6961 tgaaatgtag ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat cctgagtcaa
     7021 gaaattctca cattaccagt gacggcgagg ttccatcaac tgatgaagaa tgcgcgcaat
     7081 gagaatgtca tcgactctta taacgtaata aacagtgtga aacctaaatg cgatcttctt
     7141 tacctctgga atcgcatcat agtcttgccc tatctcgggc atttgagaga gtgtatccag
     7201 aaaagtgccg agagagtcgg tatagcgatc cgcctgctca tcaccaaact ttcccagcgt
     7261 gtagtcatag atccctctga aatcttcaac agcttttccg ctaagcttat acacgatgtt
     7321 tttgcttcct ttctgctgcg atatcacgaa gcgtcatcga actttcaccg ctctcaagac
     7381 cttcaataac agcatttctg acagcttcat ccctggattc ttgttgttcc aatagacgaa
     7441 gtgccgaacg cattacttca ctggccgagc cataacgacc agactggacc atccgggtaa
     7501 taaaactatc taattgttcg ccaacgctta ctgaggtaat tttgcgcata agcgatctcc
     7561 acgtattaac tattaataca aagtatatgt cagaacgctt tctacaacca aaccgtattc
     7621 agttgcctgt gaaacaggca aaaaaaaacc gcagcgcaaa tacgccacgg tttttttaac
     7681 agaacggatt taaaacacca ttacggcaga attgacggct gatccgcccc ttctttttcc
     7741 actttctgct gcaacatgtg ttcgcgcttc atacccagtt tcagagccaa cgcagatgcc
     7801 acatagatgg aagatgcagt accgatggaa acaccgataa gcatggtcag cgagaagcct
     7861 tccagtaccg gaccaccgaa gagatacagc atcaggataa ccatcaaggt agtaccggat
     7921 gtgatcaagg tacggtgcag cgtctgggtc aaggacacgt taaagatttc gtaaggcgta
     7981 ccgcgacgga tcttgcggaa gttttcacga attcggtccg ataccacgat actgtcgtta
     8041 agcgagtaac cgataaccga catcaacgat gccacaatgg tcaggtcaat ctcgatatgg
     8101 aataacgaca aaatacccag cgtaatgata acgtcgtgcg ccagcgcaat aaccacccct
     8161 gccgccagtc gccactcaaa gcggaaacct acgtacacga ggatagacag cagcgctgcc
     8221 atcaacgcca tcgcaccggt ttgcgcaagg tctgccccca cgctcggacc gacgaactca
     8281 atacgcttca ctgctgcatt ctgattggtg gattcgttaa tcaccttcag aacctggctg
     8341 cccagcacct gaccgccggt ttcgccttca gcaggcggca tacggaccat gatgtcgtgg
     8401 ctgctaccaa agttttgcag catcggctct tcaaaaccgg ctttttgcaa tgcatcacgc
     8461 attacgtcaa tttcagccgg tttttcgagc gtaatttcga taaccgtacc accggtgaaa
     8521 tccagccccc agttaaagcc gcgcacgccc ataataacga tagcagcgat taacagcaga
     8581 ccagagatgc caaaagccca gtagtcccag cgcataaagt catagacttt acggccgtgg
     8641 tttagttgtt caacagtata ttcctgtgcc acatcgcact cctcagattg acagcttctt
     8701 gacgcgcttg ccgccatata gcaggtttac gatggcacgc gtaccgacaa tcgcggtaaa
     8761 catcgacgtc gccacaccga taccggtagt aatcgcgaac cctttaattg ccccggtacc
     8821 cactgcgtac aggatgatga ctttaatcag cgtggtgatg ttcgcatcga agatagaact
     8881 gaatgcgcca cgataacctt catcaattgc ctgttgaaca gtacgtccgt tgctcaactc
     8941 ttctttaata cgttcgttga tcagtacgtt cgcatcgacc gctaccgcaa gggttaagac
     9001 gatacccgcg atacctggca tactcagtgt tgcgcctggc aacagcgaca taataccgac
     9061 gatcaagatc aagttggcaa tcagagcact ggtcgcaatc agaccaaact tcttatagaa
     9121 gatgatcatg aacagaatag aaaccagcag accggcaagg caagcctcca gcccctgctc
     9181 aatgttctgc atacccaggg tcgggccaat tgtacgttct tcaacaatct gaatcggcgc
     9241 gatcaacgca ccggcacgca gcagcagtga cagctgacgg gcttcgttcg ggttgttgat
     9301 gccggtgata cggaagctgt tacccagacg agactggatg ttggcgatgt taatcacctc
     9361 ttcctgtttc accagaaccg cacgaccatt tgcatctttc ttaccgctgt ctttgtactc
     9421 cacaaacagg gttgccatcg gtttgccgat gttgtccttg gtgaagttag acataatgtt
     9481 gccaccagcg ctatcgagcg agatgttaac ctgcggctgg ttgtattcgt cctggctgga
     9541 agtggagtcg gtgatatggt caccggtaag aattacgcgt ttgtacagca caaccggctg
     9601 accttcgcgg gtctgtttca cttcagagtc gcccggtacg cgaccggatg ccgcagcggc
     9661 ctgatcaacg ttggtgttta ccagacggaa ttccagcgtt gccgtcgcac ccagaatctc
     9721 tttcgcacgc gcagtgtcct gaatacctgg cagttcaaca acgatacggt cagcaccctg
     9781 acgctgaacc accggctccg ccacgccaag ttggtttaca cggttacgca ggatattgat
     9841 gttctgctgc accgcatatt cacgcgcttc actcagacga gcatcgctca ttaccgcacg
     9901 cagctggttg ctgccctgac tgctaatcac caggtccgga tgacgcttgc tcagatacgc
     9961 aatggcttca tcacgagctt tagcatcgcg gaaagtgatg ctcaggccgt agttattttc
    10021 tttacgaacg gtggtatacg ggatgccttt ttcgcgcagg tcgctgcgca ggctatcgat
    10081 attttgttcc tggagtttgc caagcgcggt gtccatatcc acttccatca ggaagtgaac
    10141 gccgccacgc aggtcaaggc cgagcttcat cggctcagcg tgaatagctg ccagccagcg
    10201 cggcgttgcc ggggcaaggt taagcgccac gacgtatttg tcacccataa cgcccattaa
    10261 tgcttcacga gcgcgcagct gggtgtcagt ggagtcgaag cgcgcaagaa tagcgccctc
    10321 ttccagtgcc acagacttag cagttatttt ttcttcttgt aacgttttct ggacctggat
    10381 cagcgtttgc tcactggcgg cgactccgcg cgcaccagtg atctgaacag ccggatcctc
    10441 accaaacagg ttgggaagcg catacagcag accgatgaca atcaccacga tcagcatgac
    10501 gtacttccac aaaggataac ggtttaacac ggcaattccc ttagggaaaa attttaatta
    10561 cagcgccttc atggtgcctt tcggcaggac ggcagctacg aagtcacgtt taataactac
    10621 ttcagtggtg tcattcagcg cgatagcaat gtagccgttt tccgctactt tggttacgcg
    10681 accaaccagg ccaccgttcg tcagaacttc atcacctttg gcaatggagt ccatcagctt
    10741 tttgtgttct ttggtgcgct tctgctgtgg acgcaggatc atgaaataga aaatcagacc
    10801 gaataccacc agcatcaaaa tcaaagacat cgggctacct tgcgccggtg cacccgttgc
    10861 cgctaccgca tcagaaataa aaaagctcat taaatttccc tcattattaa tattaatcaa
    10921 cgttcaaagg tggtacttct cgcccctgac gctggtaaaa atcagttacg aagctctcta
    10981 atttaccctc ttcaatagcc ttgcgtaaac ccgccatcaa acgctggtag taacgaaggt
    11041 tatgaatggt attgagtcgc gcgcctaata tttcgttgca acggtcaaga tgatgcaagt
    11101 aagcgcgtga ataattgcga caggtgtagc aatcacactc aggatcgagt gggccagtat
    11161 cgctcttata cttcgcattg cggattttca ccacgccatc ggtcacgaac aaatgaccat
    11221 ttcgggcgtt gcgggttggc attacgcagt caaacatatc gataccacga cgtacgcctt
    11281 caaccaggtc ttctggttta ccaacgccca tcaggtaacg cggtttgtct gccggaattt
    11341 gcgggcatac atgctccaga atgcggtgca tatctgcttt cggctcaccc acagccagac
    11401 cgccgacagc gtagccatca aaaccgatat ctaccagacc tttaacagaa atatcacgta
    11461 aatcttcgta aacgctgccc tgaatgatac caaacagcgc atttttgttt ccgagactgt
    11521 caaaacgctc acggctacgc ttcgcccaac gcagagacat ctccatggag cgttttgcgt
    11581 aatcccagtc agcaggatac ggcgtacact catcaaagat catgacgata tccgaaccaa
    11641 gatcgtactg aatctccatc gacttttccg ggtcgaggaa aatcgggtcg ccgttgatcg
    11701 ggttacggaa gtgcacgccc tgttcggtga ttttacgaat atcgccaagg ctgaagactt
    11761 ggaagccgcc ggagtcggtg aggatcggtc ccttccactg cataaaatcg tgcagatcgc
    11821 cgtgcagttt catgatttcc tggcccgggc gcagccacag gtggaaggtg ttgccgagga
    11881 taatttgcgc gccagtggct tcaacttctt ccggcgtcat cccttttacg gtgccgtagg
    11941 tgccaacagg cataaaacaa ggcgtttcca ctacgccacg atcaaagacc aggcggccac
    12001 ggcgtgcgcg accgtcggtg gtgtccagtt caaatttcat tttttctcca ctacgtcaga
    12061 aaaacagtcc aacgtttaaa ccagcgccgc ggaattactc cccgacgcgc tcattaattg
    12121 cctgcggatt gtacgtgata aacatcgcat caccgtaact aaaaaagcga tatttctctt
    12181 ctaccgccgc tttataggca ttcattgtgt gttgataacc ggcaaaggcc gaaaccagca
    12241 taatcagcgt cgattccgga aggtggaagt ttgtcaccag cgcatcgacc actttgtact
    12301 ggaagcccgg atagataaag atttgggtat cgtcaaagaa cggttcgatg agatcgtttt
    12361 tcgccgcctg agccgcgctt tccagcgaac gtactgaggt ggtgccaacc gcaatcaccc
    12421 ggttaccgcg cgctttcgcc gccagtaccg cgtctaccac atcctgcggt acttcagcgt
    12481 attccgagtg catgatgtga tcttcaatgg tgtcgacgcg taccggctgg aaggtgcccg
    12541 caccaacgtg cagggtgacg aacgccatct ccacgccttt ggcgcgtaat ttttccagca
    12601 aaggttcgtc aaaatgcaga ccagcggtcg gggctgcaac cgcgcccggt ttttcgctat
    12661 aaacggtttg ataaagttcg cggtcagcgt cttcgtccgg acggtcgata tacggcggca
    12721 gcggcatatg gccgatgctg ttgagaatat ccagcaccga gcgttcatca ttaaattcga
    12781 cttcaaacag tgcgccgtgg cgcgcggtca ttgttgcgtt aatactttcg tcatcgccca
    12841 gcagcagttc tgcgccaggt tttggcgctt tcgaggcgcg aatatgcgca agaatgcgtt
    12901 tgtcgtcgag catccgttca accagcactt caatcttgcc gccgctggct ttacgcccaa
    12961 acaggcgcgc cgggatcacc cgggtattat taaaaaccag aagatcgccg gggttgagct
    13021 tatcgagtaa atcggtgaaa gtaccgtgcg tcagcgcgcc cgtcggcccg tccagcgaca
    13081 gtaaacgaca gctactgcgt tcaggcatcg gatagtgggc aatcagggat tcgggcaatt
    13141 caaaggagaa atcggtaacg cgcatgacac tgactcagac taaaataaga ggcgggtagt
    13201 ctagtgccgg ggcgctctcc ctgcaacctt tacccccctt tggatagaat ataatcgatg
    13261 aattttttag ctcacctgca tttagcccat ctcgcggaaa gctcgctttc cggcaatttg
    13321 ctggctgatt tcgtacgcgg aaatcctgaa gaaagttttc cgcccgacgt cgtggctggc
    13381 attcatatgc atcgacgtat cgacgtattg actgacaatc tgccggaagt ccgcgaagca
    13441 cgggagtggt ttcgtagtga aactcgccgc gttgcgccta ttacgctgga tgtcatgtgg
    13501 gatcactttc tttcccgtca ctggtcgcag ctgtcgccgg actttccgct acaggaattt
    13561 gtttgttatg cccgcgagca agtgatgaca attttgccgg actcaccgcc acgctttatc
    13621 aacctgaaca actacttatg gtcagagcag tggctggtgc gctatcgcga tatggatttc
    13681 atccagaacg tgttaaacgg catggcaagc cgtcgcccac gtctggatgc cctgcgtgac
    13741 tcctggtacg atttagacgc ccattatgcc gccctcgaaa cccgcttctg gcagttttat
    13801 ccacggatga tggcgcaggc gtcacgcaag gcgctctgaa acttcaaact ccggcgtcac
    13861 tattttttag cattcgcatc cagcggggcg gcataacaac ggtaagaaaa acattcgctg
    13921 gaatgattgg cgatatcttt aaagttgctt tcagcaaccc cctcgaacat gagtaccgtt
    13981 ctggacaact ctccgttttc atttatatgt ggaaaatcaa attggctact gaatcgccat
    14041 acatctgtct tttctggctc gctcttcaca gagatatgtc gatggaggac ccctcctgta
    14101 aacgtgcgcc agaacaattg ttgatcaaaa tcttgtaata tcgcagactt ggatgctatt
    14161 tgacgtagta aacctgcata ccgtggactg ccattacgag caacattatg caaaccctga
    14221 ctaaaagcat caaacaaaca atagatgtga tcactagtga aaaaaggtct taattttttt
    14281 tgttcttcat cgctgtaatc caggagcatc tcattgattt ct
//
LOCUS       DAZFBR010000119        13793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFBR010000119 DAZFBR010000000
VERSION     DAZFBR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.119"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            51..548
                     /gene="ftnA"
                     /locus_tag="ACHJZ2_003941"
     CDS             51..548
                     /gene="ftnA"
                     /locus_tag="ACHJZ2_003941"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO4569966.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(585..824)
                     /locus_tag="ACHJZ2_003942"
     CDS             complement(585..824)
                     /locus_tag="ACHJZ2_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO4569967.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1016..2227
                     /gene="tyrP"
                     /locus_tag="ACHJZ2_003943"
     CDS             1016..2227
                     /gene="tyrP"
                     /locus_tag="ACHJZ2_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO4569968.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2289..2954)
                     /gene="yecA"
                     /locus_tag="ACHJZ2_003944"
     CDS             complement(2289..2954)
                     /gene="yecA"
                     /locus_tag="ACHJZ2_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO4569969.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3311..4312)
                     /locus_tag="ACHJZ2_003945"
     CDS             complement(3311..4312)
                     /locus_tag="ACHJZ2_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4569970.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(4318..4665)
                     /locus_tag="ACHJZ2_003946"
     CDS             complement(4318..4665)
                     /locus_tag="ACHJZ2_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFO4569971.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(4695..5345)
                     /locus_tag="ACHJZ2_003947"
     CDS             complement(4695..5345)
                     /locus_tag="ACHJZ2_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569972.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHIESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFNL"
     gene            complement(5361..5765)
                     /locus_tag="ACHJZ2_003948"
     CDS             complement(5361..5765)
                     /locus_tag="ACHJZ2_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4569973.1"
                     /translation="MKNDIGRRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(5855..5992)
                     /locus_tag="ACHJZ2_003949"
     CDS             complement(5855..5992)
                     /locus_tag="ACHJZ2_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569974.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            6064..6267
                     /locus_tag="ACHJZ2_003950"
     CDS             6064..6267
                     /locus_tag="ACHJZ2_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4569975.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            6289..6639
                     /locus_tag="ACHJZ2_003951"
     CDS             6289..6639
                     /locus_tag="ACHJZ2_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO4569976.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            6650..6928
                     /locus_tag="ACHJZ2_003952"
     CDS             6650..6928
                     /locus_tag="ACHJZ2_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569977.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGIVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            6940..7182
                     /locus_tag="ACHJZ2_003953"
     CDS             6940..7182
                     /locus_tag="ACHJZ2_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO4569978.1"
                     /translation="MGKEYKTLINKAPERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            7179..7292
                     /locus_tag="ACHJZ2_003954"
     CDS             7179..7292
                     /locus_tag="ACHJZ2_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569979.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTDK"
     gene            7385..7801
                     /locus_tag="ACHJZ2_003955"
     CDS             7385..7801
                     /locus_tag="ACHJZ2_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569980.1"
                     /translation="MNAKEKNIINTLKIVSAEQDKLSRAAQKDNQHMAALYALTIAIA
                     TPEAAKVIEEQSKEIDTLKTQSTVAAMNPSSIGRCIYILGSAMMLQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
     gene            7825..8028
                     /locus_tag="ACHJZ2_003956"
     CDS             7825..8028
                     /locus_tag="ACHJZ2_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569981.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            8025..8291
                     /locus_tag="ACHJZ2_003957"
     CDS             8025..8291
                     /locus_tag="ACHJZ2_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO4569982.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            8288..8587
                     /locus_tag="ACHJZ2_003958"
     CDS             8288..8587
                     /locus_tag="ACHJZ2_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569983.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDNVFDFFLNQ"
     gene            8910..9140
                     /locus_tag="ACHJZ2_003959"
     CDS             8910..9140
                     /locus_tag="ACHJZ2_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO4569984.1"
                     /translation="MADRKQHRAIAERRHIQTEINRRLSRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLHEFQQLIQQQNKLH"
     gene            9213..9578
                     /locus_tag="ACHJZ2_003960"
     CDS             9213..9578
                     /locus_tag="ACHJZ2_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569985.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATACRDTLNGMTK"
     gene            9585..12407
                     /locus_tag="ACHJZ2_003961"
     CDS             9585..12407
                     /locus_tag="ACHJZ2_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO4569986.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYRVENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRQKGKKSWQAERYARGNLRKIFERLERTDHRWLTQGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMTESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFTTYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPGVEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRRDI"
     gene            12484..13443
                     /locus_tag="ACHJZ2_003962"
     CDS             12484..13443
                     /locus_tag="ACHJZ2_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO4569987.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVATAIREACGVNATFIADGVPQFALVNGLYAMNKE"
     gene            13448..13762
                     /locus_tag="ACHJZ2_003963"
     CDS             13448..13762
                     /locus_tag="ACHJZ2_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO4569988.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQANQPSGVECNFYKIKKNIQTLF
                     PE"
BASE COUNT         3696 a         3634 c         3205 g         3258 t
ORIGIN      
        1 aaattatagt gacgccacag caacaaatat aacctttgtg gagcactatc atgctgaaac
       61 cagaaatgat tgaaaaactt aatgagcaga tgaacctgga actgtactct tcactgcttt
      121 atcagcaaat gagcgcctgg tgcagctatc ataccttcga aggtgctgcc gcgttcctgc
      181 gccgtcacgc ccaggaagag atgacgcata tgcagcgtct gtttgattac ctgactgata
      241 ccggcaattt accgcgtatt aataccgttg aatctccgtt tgctgaatat tcctcacttg
      301 atgaattatt ccaggaaacc tataaacacg aacaattaat cacccagaaa attaacgaac
      361 tggctcatgc tgcaatgacc aatcaggact acccaacatt taatttcctg cagtggtatg
      421 tttctgagca gcatgaagaa gagaaactgt tcaaatcgat tattgataaa ttaagcctgg
      481 caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga actctctacc ctcgacacac
      541 aaaactaatg ctctccggcg gcaggtttct gccgccattt caaatcagtg acggcaaatc
      601 ttactctgat cggtcgaaaa accttcttct gaaggtataa atttgccttt tgctgccaga
      661 aacgctacca gctctcccgc tgtcatccct tctgccgagc aagtgtgaaa acgtgcctgt
      721 tcaccaaaac gcgctttaat cgcagcttcc agactggcat gcgtatattg ctcgcctgat
      781 tcaatcatca tatttaacac ttcatgaccg tgaatagagt ccatcgttcc tcctcaaaaa
      841 aaagcctagc gtagcgattg ccgcttatga agactttgcg ccagcgcagg actgaatgct
      901 ttttattgta catttatgtt tacaccatat gtaacgtcgg tttgacgaag cagccgttat
      961 gccttaacct gcgccgcaga tatcactcat aaagatcgtc aggacagaag aaagcgtgaa
     1021 aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc acaattggcg caggcatgct
     1081 ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt acgttaatct tgttgattgg
     1141 gctttgggcg ttgatgtgct acacggcgct attactgctg gaggtgtacc agcatgttcc
     1201 ggcagatacc ggtctgggca cgctggcaaa acgctatctg ggacgctacg gtcaatggct
     1261 gacgggcttc agtatgatgt tcttaatgta tgctctgact gcggcataca tcagcggtgc
     1321 cggtgaattg ttggcctcca gcatcagcga ctggacaggt atttcaatgt cggcaaccgc
     1381 tggcgtgttg ttgttcactt ttgttgccgg tggcgtggtt tgtgtcggga catcgctggt
     1441 cgatttattt aaccgttttc tgttcagcgc caaaattatt tttctggtgg tgatgctggt
     1501 attgctgcta ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc aacaggggct
     1561 ggcactgtct gcaatcccgg tgatttttac gtcgtttggt tttcacggta gcgtgccgag
     1621 tattgtcagc tatatggatg gcaacattcg taagctacgc tgggtgttta taaccggtag
     1681 tgcgatcccc ctggtggcat atattttctg gcaggtggcg acgcttggca gcattgattc
     1741 aacaaccttt atgggattgc tggctaatca tgccggatta aacgggctgt tacaggcgtt
     1801 acgcgaaatg gtggcctctc cgcatgttga gctggcagtg catttatttg ctgatttagc
     1861 ccttgccacg tcatttctcg gcgttgcgtt aggcttattt gattatctgg cagatttgtt
     1921 tcagcgttca aataccgttg gtggacgatt gcaaactgga gcaattacct ttctgccacc
     1981 gttggcgttt gcactgtttt atccacgagg atttgtgatg gcgctgggtt acgccggtgt
     2041 ggcgctggcg gtactggcat tgattatccc ttcgctgttg acctggcaaa gcagaaagca
     2101 caatcctcaa gcgggttacc gggtcaaagg tggtcgtccg gcgctggtgg tggtgtttct
     2161 ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg gcagggttgt taccagaagt
     2221 ggggtgatta gatagcctca aattccttat tgggtgccag aattaacgct gacacccaat
     2281 ttgacttctt aatgcaggca gcactgctta aattttttac cactaccgca cgggcaagga
     2341 tcgttacgcc ccggtttctc ttctgctttg atcggttgct gaacagcttt ttcctgcgga
     2401 tgcgccatcc agtacgcatg tagatcaagc gccgccagtc gaatggcatc tacgctctct
     2461 tcaaacgctt ctggcgacat cttctctacc cgctcgaagt tttcctcagt accgtgcagc
     2521 gctatcgcct ccagcgctgg ttttaacgaa tcgggcaatg ttgaccagtc agaaagtgcc
     2581 acgccccgca tatagccaaa gcaccactcc tcaacaatcg tcagctcgct gccatcaact
     2641 tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt cgttcagacg ctctgcggta
     2701 tcggccatat gttgaaaagc caggttcata aagcgcgtca tctctttctc tgacgcccag
     2761 cgcggcacat agtcagcccc accccacacg gcaaccagcc actgtgccgg ttcaatctct
     2821 tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc aaggatggcg
     2881 tggtcagtgt tgtatttggt cagaatatcg tccagccatt ccaactcact ttcgtttaac
     2941 ggtcccgttt tcatacgctt ttccttgtgg atctcaactc gccagcacct atcttacatg
     3001 ccggtccgta tcagagatac tttttgagtg gctttgccgg tgattaaaaa tcaaggaggg
     3061 tgtaacgaca agttgcaggc acaaaaaaac cacccgaagg tggtttcacg acactgctta
     3121 ttgctttgat tttattctta tctttcccat ggtacccgga gcgggacttg aacccgcaca
     3181 gcgcgaacgc cgagggattt taaaaactat caaccaccat ttataaatca taaccttatg
     3241 attttattaa ctttgaaaat ggctctatac aacgccattt gaatctattg tcacttttta
     3301 tcgccacttt ttaccctcta ttgtcaaaag ggttcaattc aacagcagcc tctaaatgac
     3361 ctggagcaaa atgcgcataa cgcatagtca tttttatatc gctatgcccc agtatttttt
     3421 gcaacacaag aatatttcca ccccgcatca taaaatgact ggcaaacgtg tgacgtagca
     3481 catgagttaa ttgcccatca ggaagctcga tcttcgctct cttaattgca gcgtcaaaag
     3541 cctcataaca tggtgaaaat agcgctcctc gttttttagg aagcatagct tgcaattgag
     3601 gtgaaatcgg tacagtgcgg ttcttctttc ctttagtttt aacaaatgtg attcgaccgg
     3661 gcagtacttg agattgcttt aatccttctg cttcactcca ccgagcacca gtcgcaagcc
     3721 caatacggac aacaaccccc aaatctttat tccgtgactc atcacacgca atcagaaggc
     3781 gttcaatctc atctacatac agaaacgcca gttccttttc ttcctcacga aacttgcgaa
     3841 taccagtcag ggggttttca ccagaccact ccccaaggcg cttcagttcg gcaaaaacag
     3901 catgtagata tgactgctcg cgattaacgg ttgcttcact aagttttttc ttccccttgg
     3961 gattccattc tcctgatagc cttctttccc gataagtagc aaacatattt ttgtcaaact
     4021 gagaagcaaa tggatctccc agcctggaac aaatcgcctc aagtttgact ttgcgctctg
     4081 caccagagga caaggtttta ccgtacatct caaaccaacg agcaatcaac tcagaaagac
     4141 gaggaccaga accatcttga aactcgtctc caactctact attcattaaa cggcgctcat
     4201 aagagagcgc ctcacttttt gtcgcaaact gtttacgaat gcgttttccc gatgccccgt
     4261 agggataaca ttcacaaagc catttacctg atggaatctt acgaaccgac attttagtta
     4321 cttatcacat aaatcaaatg cagccttagt gacatccccc agactctttt ttaaccctgg
     4381 ggcggcatca ttatctagcc aaaatggatt attgttatct aacggtaacg caccaaatgt
     4441 tttacctttt attcgagcca aacctgtaag tgcatataac ttattatcgt caaaattcat
     4501 cacataagga ttaccatcaa gacactgtaa ttgaacctca tcagtattaa atggccatac
     4561 cccattgaaa ctctcacgtt caatagtttt aaaaggcatt gcgacggcgg aaaaagaaaa
     4621 catagataaa aaagtaacta atagttgaac cttttttact ttcatatcat tacctcaatt
     4681 tagctcaagt aagattacaa attaaaaaac gccctagaaa tgacaccgcc taccaaaacc
     4741 cctacgcaga taaagaatat tatttctttt ggataaagtc ggattaattc tgaagcacga
     4801 agtcggactt ctggtaaggt cgaactctct atgtggcttg atgccgattg ttgctctaac
     4861 cacgacaatg cagactgtaa ctgagaacga gtaagatcgt ttaaacgtcc tgtaccgaaa
     4921 ttgatatggc aataccgcag aagtttttgt cgaagtccac agtcttcact gttacgtagt
     4981 aataaactta caagagcctt acaggcatca tgatctttac atcgctcaag cattgcatgc
     5041 agaaaactct ccgctgtttt atattgattt actgtcatat catcaatacc agctacacca
     5101 atctccgcat gtactttttg ccaaataata aacgcttcag tattgctagc ttctgcaata
     5161 gcagcaacca agctatttag ctccttacgc tgagccttaa gcaaagggcg atcgtcatca
     5221 tcattattcg aagggattgc gatattgacg gtatgagaac catcatatcg ctctatctga
     5281 atattctttt cgtgaaaatc acgcccagca acgcgattgt ttgaaccgtt tgagttgacg
     5341 gccatgtcac ctccctacta tcacctaccc ttagtttcgt tatagtcacg accagcgata
     5401 cggttattac caccagaaat atttaactca cgtcctgatg gctgagtttc cttttcactg
     5461 atcgcacctt ttaaagcccc aatcaccgcg tttttcacat ctaacgaagc tgctcgaaag
     5521 cgagtaatca attcctgctc atcatcgtta taagtttcag gtgagtgaat tcccaacaca
     5581 acatactgaa catcaaggcc aaaacgagac agcgctgcca aatacgcagc atcaggaaag
     5641 ctatctcctt tctcatatct aagctgagtt agctttttga ctccaccaat gtcgctcatg
     5701 gcaacttgac taagtcccaa tctttccctt tcctcacgca accgccgacc aatatcattt
     5761 ttcatacaaa aaccttgaca ggtatctttt ttgataccaa aatgatttca cgagctatta
     5821 gatgatcaca atataccact atgaaacaag ttcttcacga taccagatca cgcattccgc
     5881 gtaacaccgc cacaggtcca agactggcac ttcggctgtc cctcgaggag cgagccgtca
     5941 ttgatgaaat ggcagctaaa gaacaacgct catcctctaa catggcgcgc atgatctccc
     6001 ttcgcggcct agagctaacc cagaaagaac aaaacaaatc ttcctgatca ggaggctagt
     6061 gggatgtcag gtataaccat caatatcaat gtgaatgccc cctatgtatc cctgcagaaa
     6121 tatgcagaga taacaggtat ccctcttaat acatgcaaaa agatgttggc tgacggtcga
     6181 attattatcc gccccaaacg cgccaaaatg gaaaagcctg aagtaaacct tgtggcgatg
     6241 ttaaaagacg ctttggctaa cagctaaaac aatgaacaga gcaccatcat gaaaaaaaac
     6301 gctaataatc catactccaa atttcgtaat ggcgtagaac gccatgtaca ccacgtcgct
     6361 accagtgcat cacgtagtaa cagtcgctat aacctgaacg agacgcatgc aacaccggat
     6421 ggccacgctg taaaacaaat cggcgaacat gcctggctga ttgagaaagc tggaatcgtg
     6481 gtccacaaat gcccacgcaa tccgtttacc gggaaccgca tttttgcatt gaactgcggc
     6541 gacaatcact tcgggcagga tttcacatta tacgaagcac ttcgcacggt tgatcgtctg
     6601 cttcgcgggc aaagttttat taaacaggct gatttataac aggtgcttta tgaccaaaga
     6661 gcatgcacaa ggtgtattta tccgttttat tgattttcgc ggtgaactgt tattacgcgc
     6721 atcagccatt gacggaattg ttccatccga aaaaaacgca gctacttacg tttatctgaa
     6781 cggtacgcgc ctgactgtgg agcttccgta ccagaccgta cgagaaatca ttagcgaagc
     6841 tgaaaaggct cgtcaggtta atggcgatga accctatatc gaaattattt gtatggattc
     6901 agaagctgaa attcagaaag cagattaaag ggcgttgcga tgggcaaaga atataaaact
     6961 ctcattaaca aagcacctga acgattttat tttcgcttaa gtgcatcagg cgctcatgct
     7021 gaacgtgcag cccgtgactc attgaccagg gcaatccgga gtttgtatga cgttgctttt
     7081 tacgctgatg atctggatgc acttaacgaa ctttccgagc tgatctgtgc cgcagaatgc
     7141 ggggaacaca ttgaaccata taagctgggg aatatcgcat gagtatattt atctcatggc
     7201 ttgttctgat tatttcggtg gcctgcgcca ttgggattat gcgaattatt cattcagtaa
     7261 aaaagattga acgctttttc actgacaaat aacagcttaa acagaacacc agattaaacc
     7321 cgaaaacctg aaaactatcc gcattcgcgg aggtattcgc acacgtaaat aacggagata
     7381 taaaatgaac gcaaaagaaa aaaatattat caatacatta aaaatagtgt cagcagagca
     7441 agacaagctg tccagggcag cccagaaaga taatcaacat atggcagcgc tttacgcact
     7501 gaccatcgca atagccaccc ctgaagcagc caaagttatt gaggaacaga gcaaagaaat
     7561 cgacactctt aaaacacagt caacagttgc agccatgaat ccgtccagca ttggacgctg
     7621 catttatatt cttggttcag caatgatgct gcaatacacc attattgccg aactgcacgg
     7681 caaatacctg ataacgcctt accacacaaa agagtcagag cttctgacaa atctccgcct
     7741 gatagaacgc tctcaagctg tattcattga tgacgcgcaa cgtgccgtat ttaacgcata
     7801 gggttactgg acaaaggggg cgcaatggca attaagcatt ttcccgtcgt tcgctttacc
     7861 tccagagggc gcgaatacga ggtcgacgaa cgcctgatta ccactatcga caaacatcgt
     7921 tcggaaaagg atgcacacca catctacctc actgacggca cttacttctg cgccaccaac
     7981 gtggcgcgag taaatcttat ccgacaggta caggagccac gcagatgacc attctggact
     8041 acatcgctac tcatccgggg tgtagcggcg gagagatcgc cgcagcactg aataccccaa
     8101 ccacagccat taatgctgag ttacgccgac tttggcgcgg cggcttagtc atcagaacaa
     8161 accgcagcac aggtggtcgc gctcgcaaaa caggaggcca ggcttcttac cacgtaaacc
     8221 cgatgccgtt cgggtgtagc aatccactta ctcacatgtt taaccagcta ctgaaggaag
     8281 ccagagcatg agcaccatca accaccagaa gctacgcgaa ctggcatttg ccctgcaacg
     8341 aatggcaacg cctcaaaaat tactggcatt tcgcgcaatg ctctcgccgt ctgctgtgct
     8401 ggcactgctg gatcagctgg agcacgccag aaccacggct cctgccattc gcctcacgct
     8461 ccatcatgaa atcgctgatt tctgcgcaac gctgggggca cctggcgaac cggaaacgcc
     8521 ggaagcaatg cagcaagagc tgctgcaacg catcgataac gtttttgatt tttttctgaa
     8581 ccagtaagaa accagaacat gcacacacaa aaaaaccgct tgccatgccg caatcggtca
     8641 ggttacattt ccgctgcacc tcataaaacg ggtgccggga ttctcaaccc gatacagagc
     8701 aaagcgcata accgcgctag cggttttttt gtgcgtactg tattgccacg tctttttcgc
     8761 gtcagaatta tggcggggcg tacggggccg acttcggtcg ggccggattc tttgctctcc
     8821 ggtgttgaga accctgtacg tctcgccacc ccgagattct caactctgga tggtgagtta
     8881 tttctatcac cgagcaaaga ggccacacca tggcagaccg caaacagcac cgcgctatcg
     8941 cggagcgtcg tcacatccag actgaaatca accgcagact ttcccgcgca tcacgcgtcg
     9001 cgcaaatcat gcacatcaat atgctgcatg agcgcagcca cgcactatca aacatttatt
     9061 ccgcctctgt tttcagctat ctggcggatg atctgcacga gtttcaacag ctcatccagc
     9121 agcaaaacaa actccattaa ttcctgttcc gggcctttcc tgcaccttgc ggcgggaggc
     9181 cttcgcacat ctgtaacaag aggattgccg caatgattct cgccaacgac tttcttgaat
     9241 acctgctcaa cacagaacgt gatcttgccg ctcgcgtgcg tgatcgttat gacatgtacc
     9301 tgaaatccct gcctgtaccg cagctcgctg acggaaagat tgttattgat ggtcgctaca
     9361 tgattgacag ccacgaggga aattacaggc tttaccgcat tgaaggtggc accccgtccg
     9421 ttattggcat ttaccagcgc ccatcctctg caatcgtcga tgtgattgcc gacagcatcc
     9481 gcatcacaca tcgccatgcc gacacagaag acaccgtgct ggaaattcag cggctggcta
     9541 cagcctgccg cgacaccctg aatggcatga cgaagtaaat cactatgacg gcagagtaca
     9601 tcagggactg gcaacaaccg cgccacgcag tggggcgtga aggaacgggg atccccgctc
     9661 ctgaatccgc gctttcctcc tggctggatg cctaccgggt agagaacgag cgccgccagg
     9721 aaatggctga tgcggcgttc tccgcaacgc cgctgggcaa cctgattaat aaaagcctgg
     9781 acgcacagga aaaacaggac aaaaccatca cactggcagg agacgccaga aaacaggcac
     9841 gcggtgcggt ggatgaagcc atggcctcgc tgcgcctgct gccgtcctat ctgcgcgatc
     9901 cgcttattcg ccacctctcc ttcctgcgca aaaaacagga agccgatcgt cagaaaggca
     9961 aaaagagctg gcaggctgaa cgctacgcgc gcggaaacct gcgcaaaata ttcgaacgtc
    10021 tggagcgcac cgatcaccgc tggctgacac agggttatcg ctcccttgcc ggacgcgaac
    10081 gcctggacga tttgctttac ctgccgcagc tcaacaaaca ccagatacag acgctggcca
    10141 ccatgacggc ggcgatgttc agcagcacct tcgaaaaact ctgcgatggc tttggcgcga
    10201 ccgatggcga actgaccatg gatgtaacgc tgaaggcgta tcagatgctg gcccgcatgg
    10261 cgttacacct gcacgccatg cctccacatt atgacgcact gacaacagac aaagaccgga
    10321 ggaacgaacc ggacacggag ctgctgccgg gcgcaatcct tcgcctgacc tgtgcggaat
    10381 ggtggaaacg caaactgtgg ctgttacgtt gcgagtggag agaagaacaa ctccgcgccg
    10441 cctgtctggt ttccagaaaa acatcgccct atctgagcca ggacgcgtta agcgagtttc
    10501 gcgcacagcg cgagaaaaca cgcgatttcc tgaaaagttt catgctggaa aacgaagacg
    10561 ggttcacgat tgatctcgag acagtgtatt acgcgggagt aagtaacccg gttcaccgta
    10621 aggcagaaat gatggccacc atgaaggggc tggaacttct ggccgaagcc cgtggcgaca
    10681 aagcggtgtt tctgactgtc acctgcccgt caaaatacca cgctacaaca gagaacggtc
    10741 atccgaatcc caaatggaac ggggccacca tgcgcgactc cagcgattac ctggttaaca
    10801 cgttttttgc ggcggtccgc aagaaactga accgcgacgg cctgcgctgg tatggcatcc
    10861 gcacggtgga gcctcaccat gacggcaccg tgcactggca tatgatggtc tttgctcatc
    10921 cggaagaaat cgacaccatt gtgtcccaca cccgcgatat tgccattcag gaagatcgtc
    10981 acgagctggg cgatgatatt actccgcgct ttaaggcgga gtatgtcgac ggctcaaaag
    11041 gcacgccaac cagctacatc gccacctaca tcggaaaaaa cctggacagc cgcgccgtgg
    11101 atggcatcga cccgaaaaca ggcaaaccac gcgttgacca cgaaaccgga aaatcaatga
    11161 ccgagagcgt ggaacgcgcc attggctggg cgcgccttca ccgggtccgc cagttccagt
    11221 tctttggcat cccctcccgt caggtgtggc gtgaactgcg tcgccttgcc agccagatgg
    11281 cacgcaaccc ggaaggcccg caacggctga aggatgacgc aatggatgcg gttcttgctg
    11341 ccgctgatgc cggatgtttt accacctaca ttgagaaaca gggaggcgta cttgttccac
    11401 gcaaggacta cctgattcgc accgcctacg acctcgcaga tgagctgaac gattacggcg
    11461 aacagagcgt acagatttac gggatctggt caccactcat cggggaatcc tcccgtgtgt
    11521 gcacgcaccc ggataactgg aagctggtaa gacgtaaacc gggagtagaa gacagcgccc
    11581 gcgaaaatgg ttttgacctt cagggcggcc ctgccgcccc ttggactcgt ggcaataact
    11641 gtccccgtgt acaggaaacg gacaacaacg ggacagaaca gccggaagaa cggccagcac
    11701 cgtggccgca gcttcctgac ggcgttgacg tgaacgaatg gatgcgctca ctgaaacggc
    11761 acgaacgccg ggcgctgatg cgttcgcttc gtgacaaaca ggcaaaaaac agcagtgatg
    11821 aaatgcagag ctggacacag agccgcaaac agcagcggcc tttgcctgat aaccacgaat
    11881 tactcgctaa agaatggcgg gagtctgctg aatctctcgg cctgcatatc ggtgaacaac
    11941 agatgcagca cctgttacgg ggcggcagtc tgtacgttga cggcagcatc attgcaccgc
    12001 agggatttga aattgtacgc aaaccggata cccgcccgga cagccgaatc acgcagctct
    12061 ggcagcgcct gagccgtaat catggcgtaa gcagcacgga gatccgccat aacccggtcg
    12121 ccagctatct ggcacagctg ggggcatcag accctgaagc cgccgcacgc ctggcatcca
    12181 cacttcagca ggaccagaac accatgaaaa cacccgttac cgtgctttct gacatgctgc
    12241 gcgccatccg cgacgcagag cacgcacaga gaatcagtga aaccactgaa cgcgccagcc
    12301 gcaaagcaga cctgctgcgg ggtggcctga ccagtggaaa caaaaaacag acagaaacgg
    12361 gactcacaaa tcccgtaaat gagcaaaaaa cgcgccgcga tatatgaagc gcgcacaaaa
    12421 caggcaaaag cgggatttaa aaatcctgta accgattaat taatcaacat aaggaaaagc
    12481 gacatgaaaa tttgtatcga cgacggctcc accaacatca agctggcatg gactgagaac
    12541 ggcgaacgcc gcaacgccat cagcccgaac agcttcaagt cggaatggtc tgcgccattc
    12601 ggtggcacgc agcccgcgaa ctacatgctt gatggcgtgc gctatggttt tgatccggtc
    12661 agcgatcgct ttgtccagac gaccgacacg caataccagt acagcgatgt gaatgtaatt
    12721 gccattcatc acgcgctggt caaatcaggc atcacaccac aggaagtgga tgtggttgtc
    12781 accctgccac tgagcgagta tttcgataca aacgcacagc cggacatggc caacatcaac
    12841 cgcaaaaaag cgaacgtcat gcgcccggtg gagtaccaga acggcgaagc attcactatc
    12901 cgtaacgtac gggttatgcc tgaatccatt cctgctggct ttaaggcact ggctgacatg
    12961 agtccgtttg aatccctgct gattgtggat ttgggcggaa ccacgctgga tgtggcaaag
    13021 gttcaggggc aactggcagg tatcagccag gtgttttgcg atccacacgt aggcgtttct
    13081 ctgatggccg atgccgtact gtcggtgatg gccactaacg gtatgcgcac cagtcaccac
    13141 atcgccaata ccattatcga acatcgccat gatgaagcct ggctgcgcca gcacatccac
    13201 aatgacgcgc attacgccag cctgatggcg gttattcgtg aaaaggaaga aacactgaaa
    13261 caacgcgtga tccgcgcgct ggcggttttt tcgggttacg ggcgggtgat ggttgtcggt
    13321 ggcggggcgg agattgtggc aaccgctatc cgcgaagcct gcggagttaa tgcgactttc
    13381 atcgcggacg gggtgccaca gtttgctctg gttaatgggt tgtacgcaat gaacaaggag
    13441 taaaccaatg acgacaccaa ccagacggat aagtttctat ctgaagcccg ccgccgtcaa
    13501 gagtgaacag gaggcgtgca attacctcga tagcctgcca gcctccgaac gcagccgcgc
    13561 gcaacgcgcg gcctttctgg ctgggctagc actcataaag cgcaacccag catttgccta
    13621 ctggatggcc gaatggccag aggacaacct ccccttcaat accgttagca tcaaggaaac
    13681 ataccaggct aatcagccta gcggagttga atgcaatttt tataaaataa aaaagaatat
    13741 tcagacctta ttcccagaat aatctgataa ttgtatgtga actaataaaa tac
//
LOCUS       DAZFBR010000120        13592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFBR010000120 DAZFBR010000000
VERSION     DAZFBR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.120"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            398..1342
                     /locus_tag="ACHJZ2_003964"
     CDS             398..1342
                     /locus_tag="ACHJZ2_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569989.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEESHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETDLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDVLLLYRFFKMNDDFNKLTSDE
                     YMSILTPLMKCERSVHDNNRYVTGKDTLLLDYPPSGNQIHFHVFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTEANKIL"
     gene            1424..1846
                     /locus_tag="ACHJZ2_003965"
     CDS             1424..1846
                     /locus_tag="ACHJZ2_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4569990.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDMAY
                     LSRCLASIHPTLAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSAISKFQHQIPVNGSVNRELLYEDYTD"
     gene            2394..2843
                     /gene="glmS"
                     /locus_tag="ACHJZ2_003966"
     CDS             2394..2843
                     /gene="glmS"
                     /locus_tag="ACHJZ2_003966"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO4569991.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            2843..4231
                     /gene="glmL"
                     /locus_tag="ACHJZ2_003967"
     CDS             2843..4231
                     /gene="glmL"
                     /locus_tag="ACHJZ2_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO4569992.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            4250..5695
                     /locus_tag="ACHJZ2_003968"
     CDS             4250..5695
                     /locus_tag="ACHJZ2_003968"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFO4569993.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            5695..6936
                     /locus_tag="ACHJZ2_003969"
     CDS             5695..6936
                     /locus_tag="ACHJZ2_003969"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFO4569994.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            6940..8310
                     /locus_tag="ACHJZ2_003970"
     CDS             6940..8310
                     /locus_tag="ACHJZ2_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFO4569995.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPALIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            8307..8624
                     /locus_tag="ACHJZ2_003971"
     CDS             8307..8624
                     /locus_tag="ACHJZ2_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFO4569996.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            8785..10065
                     /locus_tag="ACHJZ2_003972"
     CDS             8785..10065
                     /locus_tag="ACHJZ2_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFO4569997.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            10173..11825
                     /locus_tag="ACHJZ2_003973"
     CDS             10173..11825
                     /locus_tag="ACHJZ2_003973"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4569998.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            11835..12437
                     /gene="cobO"
                     /locus_tag="ACHJZ2_003974"
     CDS             11835..12437
                     /gene="cobO"
                     /locus_tag="ACHJZ2_003974"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO4569999.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(12471..13376)
                     /locus_tag="ACHJZ2_003975"
     CDS             complement(12471..13376)
                     /locus_tag="ACHJZ2_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4570000.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDTGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
BASE COUNT         3723 a         3281 c         3195 g         3393 t
ORIGIN      
        1 aatttattgt gaaatattta tgcaacacaa ttattacaat gaaaaggtat agataaaaaa
       61 ccagtatttt atttaacaga accgccttat ccacttttta taataccaat tgattagagt
      121 agccaatttg ttgctatttt acataacata tttttatcaa agttaattga ttgttgcctt
      181 tatgagcacc gaccgtaagc ccgttatgtt attgtttcat tagtaataat cacttaaatg
      241 tatcgatgaa acaggtatga cttaccggga gtgattgttt ctgagtgaac agaggccact
      301 ttcgcctgat aaatgaaaaa tgagcgattt ttgcaagtta gtttttctat agccgagtac
      361 gctcagcgcc tcgcttgatt acaatgaggt tgctgctatg agtcctgata atatcggttc
      421 ggctgtcaat cgcttctcag gttggccatc taatgattct ctaaaaacag ataaagaatc
      481 aatgacatca cccgtgactg tgcaggtaat aaagatgatt gccgccatcc ttgacccaca
      541 tcggatagtc gttggaattg atacacttta tacaacgcag cgcgaaacat ttacctcaga
      601 gagtcaacag ctcattgaat cagcattgca aacacttcat gtgatgaatt taaataatct
      661 ggaaacaaat cgcgatgaag aaagtcacta tctggctttt gaatataacg ttgacacctc
      721 tgacagaata accgtttctg cttataatga aaaaaaacct ggctgctatg gccaatgtct
      781 tgtaatacag gatgacgaaa cagacttatt taacctgata aaaattaaaa ttttcgaagc
      841 caggcataat tatgtcgctc aattgatgag cgatccggaa tttatgttca aattcagtta
      901 tagcgctcaa caaaatagat tagaacctca tcttctggtt cccgcttatt tccctctaaa
      961 aacaaacgaa ccggttacgc aagaggatgt actgttactt tatcgtttct ttaaaatgaa
     1021 tgatgatttt aataaattaa catcagatga atacatgagt atcctgacac ctctgatgaa
     1081 gtgtgagcgt agcgtccacg acaacaacag gtatgtgacg ggcaaagata cccttcttct
     1141 ggactatcct ccttctggta accagataca tttccatgtt ttccctgacg aatcagcaac
     1201 actagtcctt tatttttcaa acaatgacat tgaatgtttc gtctttgaaa gagatattcc
     1261 gagccagtat cgctttttca aaatgtttac caatctggca ctggttatag atcagttaac
     1321 cgaagccaat aaaatacttt aattttctat atatccccta tggcaaatat tgctaaacaa
     1381 tgcctgggga ttaattttta ttactcacgg aaagatttaa ataatgatac catctcagat
     1441 ctctttcaat acccttccag tcaatgcaac atacgcttca gagacaacag ttgacacaca
     1501 aaaattctct gacatcctat actcagcggg gtgttcccta aaggatatgg cttatctgag
     1561 cagatgtctg gcatcaatac acccgactct cgccaaaaac atttatgaaa cagaaaatct
     1621 cagcgatcaa aaattactac atattgattg ccgtagtaca aatgaaatca agataaatat
     1681 cttttttggt caacaacgag aaggattaat tgaaattaat tctgacacag tcattttttc
     1741 gatattgtct tcattaattg actccgccat ttctaaattt caacaccaaa tccccgttaa
     1801 cggttcagtt aatcgtgaac tattgtatga ggattatact gattaaatcc accctgtcgc
     1861 gaaaaatggc ctgcgttatt gccagacgta ataaaacctt tgattaacgt tactcatact
     1921 gatagcacct tcatgtacct ctctttttta cgaaaaatta aaagagctaa ctaaggtcat
     1981 aattcgcatg aatcattaac catttgttat tttcaacatt atttttacat taagcagaga
     2041 tgaattgcag tgaaattcga cttaaatctt atgaaatgca tttactcatg gctaaaacca
     2101 cgtcacaaaa atgcaaatta gaagtagatc acaattatct caatacatta aatcaagtta
     2161 attttaaaaa tttaacactc atgacattag ttgacataac acactgaatt aacatggaaa
     2221 tttaaaaaca cacttcaaac aatcatttcc cgaaagacat tttgaaaata gatcaattgc
     2281 aacattacgg tcagataaag acactcctgt gatattaaac ctgtgccgtt atgaacggcc
     2341 ataaatacga cgtgtgtaac ttattttact ctctgtctta caaaggatta cccatgaaga
     2401 aagcaacact tgtgattggc gttattggcg cggactgcca tgcagtaggc aataaagttc
     2461 tggaccgcgt ttttagcaac cacgacttca gagtcattaa tctgggcgta atggtcagcc
     2521 aggacgaata tattgatgct gccattgaaa ctggcgctga cgcgattgtc gtctcctcta
     2581 tctatgggca tggcgatatc gactgtctgg gcatgcgcga acgctgcatc gagcgtggac
     2641 tgggggacat cctgctctat gtgggcggta acctggtggt cggaaaacat gacttcgctg
     2701 acgtggaaac caaattcaaa gagatgggat ttgatcgcgt ctttgcccca agtcatgact
     2761 tagaagatgt ttgccagctg atggcccacg atatcaacca acgtcatgac gttgatactc
     2821 gtattcttga agaggctatc tgatgcaaat cgtctctgtc gatattggat cgacgtggac
     2881 caaagcagcc ctctttaccc gggaggggga cgcgttaacg ttggttaacc acgtactgac
     2941 cccaaccacc acccatcatc tggcgaaggg gtttttctcc agcctgaacc aggtgttaaa
     3001 cgtagataat gccctccccc tgttaaacag cggtgaggtt gcattgaagt attcctcctc
     3061 tgccaaaggt gggctggcgg ttgccgcaat gggattggtg ccttctatca ccctggaaac
     3121 cgcgaaagta accgctcact ctgcgggtgc taaaattgcg caatattacg cctacaagct
     3181 gaaccgccgt gatatccagg cactggaaga aacgcagccc gatattctct tgttcaccgg
     3241 cggcaccgac ggcggagaag aaagctacgg actgaataac gcccgtgtgc tggcagaatc
     3301 caaactcgac tgcgccatta tttatgccgg aaaccgggat attcaggacg aggtgcagga
     3361 gatcctcggg cataaagacc tgaccattgt cgacaacgtt ctgcccgatc ttgaccatcc
     3421 gaaccctgta gctgcacgcc aggctatttg cgacatattc cttaaacgca tcgttaaagg
     3481 gaaagggctg gatgtcattg ttgataaaac aggcgaagaa ccaatgccga caccatggac
     3541 ggtctttgaa ctggtcaagg caatcagtaa cgtcgatcac agctggaaag agttcatgct
     3601 gatcgacatg ggcggcgcaa ccaccgatgt ttattccgcc tgtgccaaca cgctctcgcc
     3661 cgacaccgtt ttacacggcg taccggaacc cttcgtaaaa cggacggtgg aaggcgatct
     3721 gggaatgcgc gtctcggcag tggtggttgg tgaaagtgcg aaagagttag tcaatgtgaa
     3781 ttttgctcag caacccgcac agctggacgc tttttatcat tacctccgtc atctagtcgc
     3841 tcatcccgac tacttacccg caaatgcaga agagaaatat ttcgataccg tcctggccgg
     3901 actgtgtgtg ggttatgcca ccgaaagaca tgcgggcacc aaaaaagagg tctgcacctg
     3961 cgtcggcaat gtcgatttac agatgggacg tgacctgaca accgtgcgta aagtcgtcgg
     4021 atccggcgga tggctctcgc gtgccagtca gttcgatatg catcactggt tgaaataccg
     4081 cgagttaaac gacgacggta aacgcattct cttaccgacc gaatttgaat attaccgcga
     4141 ttcccggggg ctattaccac tgctggcaaa cgtcgccaga gtcgatccgc tggccgccgc
     4201 gcgcaccagt attcaatgct taaccctata acaattaagg ctgcaatcaa tggaacttcg
     4261 aaataagaaa ttaacccatg acgaattcat gaccgagcgg caacaggtac tgaaaacctg
     4321 ggaaaccggc aaggatgttg aaaactttga agatggcgtt aaataccaac aaaccatccc
     4381 tgagcataaa cgtttctctc ttgcgctgct gaaagcagac aaggaaggta aaacccttag
     4441 ccaaccgcgc gcaggtgtgg cgctgatgga cgaacacatc gaactgctga aaacactaca
     4501 agaagagtgc gatctgctgc caagcaccat cgatgcatat acccgtctga atcggtacga
     4561 agaagcggct gtagggatta aaaaatccat cgaagccgga acctctaaac tgaacggttt
     4621 accggtagtt aaccatggtg tcgcagcttg ccgtcgcctc accgaaaccc tgcaaaaacc
     4681 gctacagatc cgccacggca cgccagatgc gcgtctgctg gctgaaatct caatggccag
     4741 cggctttacc agttatgaag gcggtggcat ttcctacaac attccgtatg ccaaacgcgt
     4801 aacgctggaa aaatcgatcc gcgactggca gtactgcgac cgtttaatgg ggatgtatga
     4861 agaacacggg atccgcatca accgcgaacc gtttggccca ctaacgggta cgctgatccc
     4921 gccgtttatc tcgcactcta tcgcgataat cgaaggcttg ctggcgctgg aacagggtgt
     4981 gaaatctatc accgttggtt atggccaggt cggcagcctg acgcaggatg ttgcggcgat
     5041 ccagtctctg cgtgaacttg cacacgaata ttttcagagc tatggctata ccgactatga
     5101 attgagtacc gtgtttcacc agtggatggg cggctttccg gaagacgaat cgaaagcgtt
     5161 cgctattatc tcctggggcg cagccgtcgc ggggatgtct ggtgccacca aagtcatcac
     5221 caaaagtccg cacgaagcct ggggtatccc gactgccgct gccaacattc aggggctgaa
     5281 agcctcccgc caaatgctca acatggtcaa tgagcagaaa ttcccgccgt gccctgcggt
     5341 tgaactggaa attgaactga ttaagagcga ggttcgcgcg gtactgaata aagtgttcga
     5401 actgggtaat ggtgatattg cgcgcggaac ggtgctggca tttgaagcgg gcgtactgga
     5461 tgtgccgttt gcgccagccg cctgcaacgc gggcaaaatt cttccggtcc gtgacaacac
     5521 cggcgcgata cgcgtactgg aagccggagc cgttccattg ccaaaagata ttctcgacct
     5581 ccaccacgat tacgttgcgg aacgcgcacg ctgtgaagga cgccaaccga cattccagat
     5641 ggttgtagat gacatcaatg ctgtatccca cagtaaatta ataggaagac cataatgaaa
     5701 ataaaacagg ctctgttcac cgctggctac tcctcattct atttcgatga ccagcaggcg
     5761 ataaaaaacg gagcgggtca tgacggcttt ttttataccg gggagccagt aacacagggg
     5821 tttaacgccg tacgtcaggc cggggagtgc gtatcggtac agttgattct ggaaaacggc
     5881 gcggtcgccg taggtgactg tactgccgta cagtattccg gggcaggcgg tcgcgatcca
     5941 ctgttcctcg cagagcactt tattccgttc ctcaacgacc atatcaagcc attactggta
     6001 ggccgcgatg tggatgcttt cctgccgaat gcccgtttct tcgacaaatt gcgtattgac
     6061 ggcaacttgc tgcataccgc cgtgcgctac ggattatcac aggcgctgct tgatgctacc
     6121 gcgctggcaa ccggccgtct gaaaactgaa gtggtctgtg atgaatggca gttgccacgc
     6181 gtggcggaat ccattccatt atttggtcag agcggcgacg atcgatatat cgccgtcgat
     6241 aagatgatcc ttaaaggcat cgacgtgctg ccccatgcgc tgattaataa cgtcgaagag
     6301 aagctgggct ttaaaggtga aaaactgcgc gaatatgtcc gctggttgtc ggatcgcatt
     6361 ctaagcaagc gcaccagcgc acgctaccac cctaccctgc acatcgatgt atacggcact
     6421 atcggtctga tcttcgatat ggatccgctt cgctgtgcgc aatacatcgc cagcctggaa
     6481 aaagaagcgc aaggcctgcc gctctacatc gaagggccgg tcgatgccgg taacaagccc
     6541 gatcaaattc gcctgctgac cgcgattacc aaagagctga cgcgcctcgg ttccggcgtg
     6601 aaaattgtgg ccgatgaatg gtgtaacacc taccaggata ttgttgattt cactgatgct
     6661 gccagttgtc acatggtgca aatcaaaacc ccggatctgg gcagtattca caacatcgtc
     6721 gatgcggttc tttactgcaa cagccacagc atggaagcgt accagggcgg cacctgcaat
     6781 gaaactgatg tcagtgcccg cacctgtgtc cacgtcgccc ttgccgctcg ccccatgcgt
     6841 atgctggtaa aaccagggat gggctttgac gaaggcctcg atatcgtctt caacgaaatg
     6901 aatcgtacta tcgcgctgtt gcaggctaag gattaacaaa tggctcgttc ttttaaagtc
     6961 ttatcgccaa cggcaatcct cgggtatgga ttcccggaag agagcttccg caaagcgatg
     7021 gcagaatcgc ccgctctgat cgccgttgat gcaggctctt ccgatccagg cccccactac
     7081 ctcggggcag gcaaaccgtt taccgatcgc gcaggggtca agcgcgatct gcgctatatg
     7141 atcaccgcag gcgtgcaaaa taatataccg gtggtgatcg gaacggctgg cggttctggt
     7201 gccgcaccac acctggagtg gtgccgagaa ataattcatg agattgcccg ggaagagcac
     7261 ctctcgttct cgatggcgtt gatcccggca gacgttgaca aagccatcgt ccaccaggcg
     7321 ctggataacg gcaaaatcac ggcgctggat tttgtcccgc cgttaaccca cgacgcgatt
     7381 gacgaaagta cgtatatcgt cgcgcaaatg ggcatcgaac ccttccagcg ggcgctgaaa
     7441 gaacgcgcgc aagtggtgct gggcgggcgc gcttacgacc cggcctgctt tgctgcgctc
     7501 ccgatcatgc aaggctttga tgaaggtctg gcgctgcatt gtgggaaaat ccttgaatgc
     7561 gcggcaatcg ccgcaacgcc cggctcaggc tctgactgtg cgatgggcat cattgatgac
     7621 aacggcttta cgctgaaaac atttaatccg aagcgtaaat ttaccgaaac gtcagcagct
     7681 gcacacacac tgtatgagaa atccgatccc tacttcctgc caggacctgg cggcgtgttg
     7741 aacctgaaag gctgcacatt taaagcagta aatgacggcg aagtctacgt cagcggttct
     7801 aagcatgaag aaacgccgta tgccctgaaa ctggaaggtg cacgacgggt gggcttccgc
     7861 tgtctgacca tcgccggaac gcgcgacccg attatgatcg ccgggatcga taaaatcatc
     7921 gatgaagtca aaaccagcgt ttcgcgtaac ctgtcgctcg acgatgacag cattcgcatc
     7981 aatttccacc tgtacggcaa aaacggggtg atgggagacc atgaaccgat gcaaactgcc
     8041 gggcatgagc tggggattgt ccttgatgta gttgcaccga cccaggaaat tgccaacagc
     8101 gtttgctcgc tggtgcgctc taccatgctg cactacggct atgaaaaccg catcgctacc
     8161 gcaggtaatc tggcgttccc gttctcccct tctgatatcc agggcgggcc ggtatacgaa
     8221 ttttccatat atcacctgat tgaagccaac gacgctctgc gttttgattt ccatattgaa
     8281 caggtgacgc cagaaggagt tcaggcatga aacagtcaat tgtctcactg gcgcaagtca
     8341 ttcgttcgaa aaatgccgga ccttacgaac tggttttaga tattttattt aaaacgaaag
     8401 aaaactatga gcgagttaaa tcatcagggc aattaacccc agaactgatt gcacgcttat
     8461 atcatgttga acctgacttt attcatcgta ttgtctggtt tgatccttct aacgcagtaa
     8521 aaatcgtcat gccacgagat attatctccg ggaatgtagg tgacaatgat gtctatggtg
     8581 cacagcaaca tgcaccttta ttaaatatga gtttcgattt gtaaaaatcc gaatactttt
     8641 agccaactga catcccgtca cttattgcag ttcaacactt cagctatttg taaggttgta
     8701 atagcaggta gatgcctgaa tctctattca ggcatctact tctcctgatt ctgatttatc
     8761 gccgtgcacc tgatggagga aaaaatgaag aaaataagtt taaccacgat gattcttttg
     8821 gcgctggtac ttggaatgat tatcggcgta gtgctcaata acactgcttc accggaaacc
     8881 gcaaaactct atgcgcaaga aatatcgata ttcacgacga ttttcttacg actgataaaa
     8941 atgattatcg ctccgttagt ggtctctacc ctggtggtag gtattgctaa aatgggcgat
     9001 gccaaagccc ttggtcgtat tttttctaaa acactctttt tatttatttg cgcctcattg
     9061 ctgtcaattg ccttaggctt gataacggta aatttcttca tgccaggtac aggaattaat
     9121 tttgttgcac acggtgccga aaccaccgga gtggtcgcgg cagaaccctt tacgctaaaa
     9181 gtatttattt cgcatgcttt ccccaccagc attgtcgatg ccatggcgca caatgaaatt
     9241 ttgcaaatcg tggtgttctc aattttcctc ggctgtagcc tgacggcgat tggcgagaaa
     9301 ggcagcgcca tcgttcacgc cttagattcg ctggcacatg ccatgttaaa gctcactggc
     9361 tacgtcatgc tcttcgctcc cctgaccgta ttcgctgcta tttcagcatt gattgctgaa
     9421 cgaggactgg cagttatggt gagcgccggg atctttatgg gtgaatttta tttcaccatg
     9481 ttattacttt gggtactgct tatcggtctg gccatcgttt atgtcggccc ctgcatcaga
     9541 cgcctgaccc gcgccctttc ggaacccgcc ctgctggcat ttaccacatc cagttctgaa
     9601 gcggcttttc cgggaacgct tgaaaaactg gagcaatttg gcgtttcccc caaaattgcc
     9661 agctttgtct tacccattgg ctactcattt aatctcgttg gatcaatggc ctactgctcc
     9721 ttcgccacag ttttcatcgc ccaggcctgc aatatccatt tatccatcgg tgagcaaatc
     9781 accatgctgt tgatcctgat gttgacctcg aaaggaatgg ctggcgtacc acgcgcctca
     9841 atggtggtta tcgccgccac gctcaaccag ttcaatattc cggaagcggg gctgatcttg
     9901 ctgatgggcg ttgatccgtt ccttgatatg gggcgttccg cgacaaacgt catgagcaac
     9961 gcaatgggcg ctgcgatggt gagtcggtgg gaaggcgaac atttcggcga gggctgtcgg
    10021 ggtaaagcat taaaacccaa tgaatcgaac gttgctctgc cctgaggggg atcttaagca
    10081 atcagaagcc aatgactacg catttttata agccggatca tgaagttaga cggcaattaa
    10141 gtaacactaa cgtttaaggt gaaaagtgaa atatgtccaa accatttatc tggcaagaac
    10201 cgtttctaca aaataaagac ggtacagaat atacattaat tagcgatcaa catattacag
    10261 taacggaatt agacggcgaa gaagtgataa aaatcgctcc tgaagcatta acgctgcttg
    10321 cccggcaggc attttatgaa gcctcttttt tcttacgttc cgctcatttg caacaggtcg
    10381 ccagcattct taacgatcag caggccagca gtaatgataa atacgttgcg ttgcagttat
    10441 tacgtaatgc tgaagtctca gcaaaagggg tattacccaa ctgtcaggat accggcaccg
    10501 caactattgt tgccagtaaa gggcaacaga tatggaccgg aggtaacgac gccgaagcct
    10561 taagtaaagg catttacagt actttccagg aaaataatct gcgcttttca caaaatgcgc
    10621 cgctggatat gtataccgaa gtcaatacgc aaaccaatct cccggcgcag attgatatca
    10681 gcgctgtcgc tggtgatgag taccattttc tctgtgttaa taaagggggc ggctccgcca
    10741 ataaagccgc gctttatcag gaaaccaaat ccttattgca accagaaaaa ctgaccgcat
    10801 ttttaatcga aaaaatgaaa tcgctgggca ccgctgcctg cccgccttac cacatcgcct
    10861 ttgtcgtcgg agggctttca gccgatcaaa ccttaaaaat tgccaagctg gcctcaacga
    10921 aatattacga caatttgccc accagcggaa acgagcaggg acaagcattc cgtgatattg
    10981 agctggaaaa agtgttgctc gaggccagtc agcaatttgg tattggtgcg caatttgggg
    11041 gtaaatattt cgctcatgat attcgcgtca ttcgtctgcc gcgtcacggt ggctcttgcc
    11101 cgatagctat ggcgctctcc tgttctgccg atcgcaatat caaagcaaaa attaacaaac
    11161 acggtatctg gctggaaaaa ctcgaacaca atccggggca atatattcca gcctcactgc
    11221 gggaagaaaa tcacgcgcaa catgtacaac tggatctaaa ccgtccgttg cgtgacgtga
    11281 tgcaggatct cgccagatta ccagtaggca cccgcgtgtc gttaagtggt cccatcgtag
    11341 tggcgcgcga tatcgcccac gccaaaataa aagcgcggct ggatagcggc gaaccgatgc
    11401 cggagtacct gaagcatcat attgtctatt acgctggccc ggcgaaaaca ccggagaaca
    11461 tggcctgcgg ttcactgggt ccaaccacgg gcggtcgaat ggacggttat atcgatacct
    11521 tccaggcggc tggcggcagt ctagtgatgt tatcgaaagg taaccgcagc cagcaggtca
    11581 ccgatgcctg tcacaaacat ggcggcttca atctgggaag tattggcggt gcagctgcgt
    11641 tactggcgca agaatatgtc aaaagtctgc gttgcctgga atacccggag ctgggtatgg
    11701 aagcggtgtg gatgatggaa gtggaaaatc tgcccgcatt catcctggta gatgataaag
    11761 gcaataattt ctttagtcag tttgagcagc aacaccgctg cgccagttgc cctgcgggac
    11821 attaaggaaa atgaatggaa gcgcgaattt caactgaacg gcatgtgcag cgccaacaga
    11881 aattgaagga acaggtcgat acgcgcgtcg cggcggcgat agagaaaaaa gggatcttga
    11941 ttgttttcac cggcaacggc aaagggaaat ccactgccgc gtttggtact gtgacgcgcg
    12001 cggtgggtca tggcaaaacg gtgggggttg cgcagtacat caaggggcaa tgggacaacg
    12061 gcgaatacaa ccttctccag ccgctggggg ttgagtttca tattatggga accggtttta
    12121 cctgggagac acagaaccgg caagcggata tcgacgcggc gaaagaggtc tggtcggaaa
    12181 gcaaacggat gctggctgac aagcgttacg acttagtggt gcttgatgag ctgacctata
    12241 tgctggctta ccattatctg gataccgaag aagtgatcgc ttctctgcaa aaccgcccgg
    12301 cacaacaaag tgtcattgtc accggacgcg gttgccacag tcagatatta aaaatggcag
    12361 atacggtcag cgaaatccgc cccgtcaaac atgcttttga taacggcatt caggcccagc
    12421 cgggtatcga ctggtaataa ctgcgcctgg catcccgcca ggcgcaggtt ttacatctcc
    12481 tgcggtacga ccgatttcgc cagcaaccat tcacgaaatt gctgcaagtg cggtgagtca
    12541 gacttctctt cctgccacgt cataataaaa cgattaccgg tacgaatagg cacatcacag
    12601 ggaatcacca tatcgccact atccaaatca tgttgaatag cgaaacgtgg taacagcgcc
    12661 acgccgagat tagagcgcac ggcggcaatc agcattgaaa gcaaatcgaa acgtggtccg
    12721 ttgttgacca gcggactgct aacgccagat aaagcaaacc actcttccca gccagtaata
    12781 cgggtgcttt gatgcagaag tggtaactca gtaagcagtt ctgccactga cagtttttga
    12841 tcggacattg caagcaaact gccgctgcat accggcaaaa tctcctcttc aaagagatat
    12901 tctacttccg cccagggcgc gcaaaaattt tcgcgcataa tgacagcatc gtactcccga
    12961 ttaagaaaat cgccgttatt agctaaagaa tgaatattaa caataatatc gggatgtaac
    13021 tttgtgaatt catgtaaatt tgggattaac cagtgggtac taaaagtagg attaacggca
    13081 agttcaataa cctgaaccgt aggctgccag gtcataatgg tattggtatc gcgttccagc
    13141 ttatttaatg tttcttttac tatccccagg taatacttcc ctgtatcatt aagaaaaatg
    13201 cgttttttag agtgtgaaaa taacggagta cgtaaaaatt cctccagcgc acttacttgt
    13261 ctgaatactg cactttgggt taacgctaac tcctctgccg ctctggtata actttcatga
    13321 cgtgcgacga cctcaaaagt caccagcagt tccgttttag gtattttccc tcgcataatg
    13381 ccgtccctta aaaatagttt aaaccatcaa aaatacatta cttattacga agtaatggta
    13441 accaaagcag caataaataa tttataagaa tcatgattga catttataaa ccagtaaggc
    13501 tggcgtgaca aatatcttaa tcagataata ataatccgaa aagcacagcg cctttcgaat
    13561 tatccggaat cacattaagt gaaaattatt aa
//
LOCUS       DAZFBR010000121        13039 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFBR010000121 DAZFBR010000000
VERSION     DAZFBR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.121"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            180..893
                     /gene="aphA"
                     /locus_tag="ACHJZ2_003976"
     CDS             180..893
                     /gene="aphA"
                     /locus_tag="ACHJZ2_003976"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO4570001.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1004..1420
                     /locus_tag="ACHJZ2_003977"
     CDS             1004..1420
                     /locus_tag="ACHJZ2_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO4570002.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1424..1780
                     /locus_tag="ACHJZ2_003978"
     CDS             1424..1780
                     /locus_tag="ACHJZ2_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO4570003.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(1815..4637)
                     /gene="uvrA"
                     /locus_tag="ACHJZ2_003979"
     CDS             complement(1815..4637)
                     /gene="uvrA"
                     /locus_tag="ACHJZ2_003979"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO4570004.1"
                     /translation="MDKIEIRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATITEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            4892..5428
                     /gene="ssb1"
                     /locus_tag="ACHJZ2_003980"
     CDS             4892..5428
                     /gene="ssb1"
                     /locus_tag="ACHJZ2_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO4570005.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5527..5808)
                     /locus_tag="ACHJZ2_003981"
     CDS             complement(5527..5808)
                     /locus_tag="ACHJZ2_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO4570006.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6237..7823
                     /gene="pdeC"
                     /locus_tag="ACHJZ2_003982"
     CDS             6237..7823
                     /gene="pdeC"
                     /locus_tag="ACHJZ2_003982"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO4570007.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIVPVNGYTIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(7826..8149)
                     /gene="soxS"
                     /locus_tag="ACHJZ2_003983"
     CDS             complement(7826..8149)
                     /gene="soxS"
                     /locus_tag="ACHJZ2_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO4570008.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8235..8699
                     /gene="soxR"
                     /locus_tag="ACHJZ2_003984"
     CDS             8235..8699
                     /gene="soxR"
                     /locus_tag="ACHJZ2_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO4570009.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9245..10594
                     /gene="ghxP"
                     /locus_tag="ACHJZ2_003985"
                     /gene_synonym="yjcD"
     CDS             9245..10594
                     /gene="ghxP"
                     /locus_tag="ACHJZ2_003985"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO4570010.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10745..12394
                     /locus_tag="ACHJZ2_003986"
     CDS             10745..12394
                     /locus_tag="ACHJZ2_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO4570011.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12548..>13039)
                     /gene="espX5"
                     /locus_tag="ACHJZ2_003987"
     CDS             complement(12548..>13039)
                     /gene="espX5"
                     /locus_tag="ACHJZ2_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFO4570012.1"
                     /translation="NLFRADLSFSKLDLINFKHADLSRVNLNKAILQNINLIDSKLFF
                     TRLTNTFLEMVICTDSNMANVNFNNANLNNCHFNCSVLTKAWMFNTRLYRVNFDEASV
                     QGMGISILRGEENIPINSDTLVTLQKFFEEDCTSHTGMSQTENNTHEVAMKITADIMQ
                     HAD"
BASE COUNT         3040 a         3197 c         3425 g         3377 t
ORIGIN      
        1 attttttaca aaaaaacgac aggaacgtat ttactaggtt aaatataatc atcctgcttt
       61 tcatcacaaa aaccgcagat aatccttcct ttcccctgta gctggcgtta tggtcagata
      121 gtttttgcaa caaatctcac aataaaaagt ttcaacatac tgactattta gggaaaaata
      181 tgcgcaagat cacacaggca atcagtgccg tttgcttatt gttcgctcta aacagttccg
      241 ctgttgccct ggcctcatct ccttcaccgc ttaaccctgg gactaacgtt gccaggcttg
      301 ctgaacaggc accaattcat tgggtttcgg tcgcacaaat tgaaaatagc ctcgcagggc
      361 gtccgccaat ggcggtgggg tttgatatcg atgacacggt acttttttcc agtccgggct
      421 tctggcgcgg caaaaaaaac ttctcgccag aaagcgaaga ttatctgaaa aaccctgtat
      481 tctgggaaaa aatgaacaac ggctgggatg aattcagcat tccaaaagag gtcgctcgcc
      541 agctgattga tatgcatgta cgccgtggtg acgcgatctt ctttgtgact ggtcgtagcc
      601 cgacgaaaac agaaacggtt tcaaaaacgc tggcggataa ttttcatatt cctgccacca
      661 gcatgaatcc ggtgatcttt gcgggcgata aaccagggca aaatacaaaa tcgcaatggc
      721 tgcaggataa aaatatccga attttttatg gcgattccga taacgatatt accgccgcac
      781 gcgatgtcgg cgctcgtggt atccgaattc tgcgtgcctc caactctacc tacaaaccct
      841 tgccacaagc gggtgcgttt ggtgaagagg tgatcgtcaa ttcagaatac tgacagagcg
      901 ggagagcgtg acactctccc gaatgctgtt tttttaatca cacctttatc ctttcgctgt
      961 cttgctgcaa actgattaag agagttttat caaggagcag cacatgtggt atcaaaagac
     1021 gctcacgctt agcgccaaat ctcgtgggtt tcatctggta acggatgaaa ttctgaatca
     1081 gctggctgat atgccgcgcg ttaacatcgg cttactgcat ctgttgctgc aacatacctc
     1141 cgcctctctg acacttaatg agaactgcga tcccaccgta cgccacgaca tggagcgttt
     1201 tttcctccgc accgttcccg acaacggaaa ttatgagcat gactatgagg gagcagacga
     1261 tatgccttct catatcaaat cctcaatgct gggaacatcg cttgtattgc cggtgcataa
     1321 agggcgtatt cagaccggca cctggcaagg catttggctg ggggaacatc gcatccacgg
     1381 cggatcgcgt cgcatcatcg cgacactaca aggggagtaa aaaatgacca tttcggagtt
     1441 gctacaatat tgcatggcaa aaccaggcgc agaacagagc gtgcataacg actggaaagc
     1501 gacgcagatc aaagtggaag atgtactgtt tgcgatggtg aaagaagtag aaaatcgccc
     1561 agctgtttcg ctgaaaacca gcccggagct ggcggagctg ctacgtcagc agcacagcga
     1621 tgtgcgtcca agccgccatc tgaataaagc gcactggagc accgtgtatc tcgacggttc
     1681 gctgccggat tcgcaaatct attatctggt ggatgcgtct tatcagcagg cggtgaattt
     1741 actgccggaa gaaaaacgta aattgctggt gcaactctga aaggaaaagg ccgctcggaa
     1801 ggcggcctta acgattacag catcggcttg aggaagcgtg ccgtatgcga agcttcgcac
     1861 tccgcgacgg tttctggcgt accggagacg aggatctcgc cgccgccact gccgccttcc
     1921 ggccccaggt cgacaatcca gtcagcggtt ttaatcacgt cgagattgtg ctcaatcacc
     1981 acaatggtgt tgccctgatc gcgcagttta tgcagtacgt cgagcagttg ctgaatatcg
     2041 gcgaagtgca gaccggtggt cggctcgtcg agaatataca gcgtctgccc ggtgccgcgt
     2101 tttgacagct cacgcgccag cttcacgcgc tgggcttcac caccagaaag tgtggttgcg
     2161 gactgcccca agcgaatgta cgtcagacca acgtccatca acgtttgcag cttacgcgcc
     2221 agagctggca ccgcatcaaa gaactcacgc gcctcttcga tggtcatatc cagcacttcg
     2281 tggatggttt tgcctttgta cttaatctcc agcgtttcac ggttatagcg tttacctttg
     2341 cactggtcgc acggcacgta aatgtccggc aggaagtgca tctccacttt gatcacgcca
     2401 tcgccctgac aggcctcgca gcgtccgcca cggacgttaa agctgaaacg tcctggcgta
     2461 taaccacgcg cacgggattc cggtacgccc gcaaacagtt cgcgcacagg cgtaaacacg
     2521 ccggtatagg tcgccgggtt agaacgcgga gtacgaccaa ttgggctttg gtcgatatcg
     2581 attactttgt cgaaatgctc cagtccctga atatcgcgat acggtgccgg ttcggtgatg
     2641 gtcgcaccat tcaactggcg ttgggcaatc gggaacagtg tgtcgttaat cagcgtcgat
     2701 ttaccggaac ctgaaacccc tgtgatgcag gtaaacagac cgactggcag cgttagcgtc
     2761 acgtctttca ggttgttgcc gcgtgcgcca gtcagcttaa gtactttttc cggattcgcc
     2821 ggaacgcgtt tcttcggcac ttcaatcttg cgtttaccgc tcatgtactg tccggtcaac
     2881 gactccggca ccgccataat cgcttccagc ggaccttccg cgaccacttc accgccatgt
     2941 acacctgcac caggaccgat atcgatcaca tgatcagcgg cgcgaatcgc gtcttcgtcg
     3001 tgctccacca caatcacggt attaccgaga tcgcgcagat ggataagcgt acccaacagg
     3061 cgctcgttat cgcgctggtg caggccgata gacggctcat ccagcacgta cataacgcca
     3121 accaggcccg caccaatctg gctcgccaga cggatacgct gggcttcacc gccggaaagc
     3181 gtttctgccg agcgggaaag cgtcaggtaa ttcaggccga cgttaacgag gaatttcaaa
     3241 cgatcgccga tctctttaag aattttttcc gcaatcttcg cccgctgacc tgcgagtttg
     3301 agattgttga agaattccat cgcatgacca atgctcatgt cggagatggc gggcagcggc
     3361 gtattctcga catacacgtg gcgcgcttcc cgacgcagac gcgtcccttc gcagctggcg
     3421 cacgggcgat tgctgatgaa cttggctaat tcttcacgta ccgcactgga ttctgtctct
     3481 ttataacggc gctccatatt gtgcagcacg ccttcgaacg gatgacgacg gatggaggta
     3541 tcgccacgat cgttcatgta tttgaattca atgttttctt tgccagaacc gtacaacacc
     3601 actttatgca cgttcgcgct caggctgccc cacggcgctt cgacgtcgaa cttatagtga
     3661 tctgccagcg atttcagcat ctggaaataa tagaagttgc ggcgatccca gccacggatc
     3721 gcaccacctg ccagcgacag ttccggattc tggatcacgc ggtcaggatc gaaatattgc
     3781 tgtacgccaa ggccgtcgca ggtcgggcag gcacctgccg ggttgttaaa cgaaaacagt
     3841 cgcggctcca gttcacgcat actgtagccg caaattgggc aggcgaagtt ggcggaaaac
     3901 agcagctctt ccgctttcgg gtcgtccata tccgccacta ccgcggtacc accggaaagc
     3961 tccagcgcgg tttcaaacga ctcggcaaga cgttgggtaa gatcgtcacg caccttgaag
     4021 cgatcaacca ccacttcaat agtgtgtttc ttttgcagtt ccagtttcgg cggatcggaa
     4081 agatcgcaga cttcgccatc aatacgagca cggatgtaac cctggcttgc caggttctcc
     4141 agcgttttgg tgtgttcgcc tttgcgctct ttaatgattg gcgcgagcag catcagacgc
     4201 ttgccttccg gctgcgacag cacgttatcc accatctggc tgacggtttg cgccgccagc
     4261 gggacgtcgt ggtccggaca gcgcggttcg ccgacgcggg cgtacaacaa acgcaaatag
     4321 tcgtggattt cggtgattgt ccccaccgta gaacgcgggt tatgagacgt cgatttctgc
     4381 tcaattgaga tggcaggaga aagcccctca atatgatcga cgtccggctt ttccatcagt
     4441 gacagaaact gccgcgcgta ggcggaaagg gattcaacgt aacggcgctg cccttcggca
     4501 tataaggtgt cgaaagcgag cgaggatttg ccagaacccg aaagcccggt cacgacaatg
     4561 agcttgtcgc gggggataac gaggttgatg tttttgagat tatgggtgcg ggcgccccga
     4621 atttcgatct tatccattca cctttcccgg attaaacgct tttttgcccg gtggcatggt
     4681 gctaccggcg atcacaaacg gttaattatg acacaaattg acctgaatga atatacagta
     4741 ttggaatgca ttacccggag tgttgtgtaa caatgtctgg ccaggtttgt ttcccggaac
     4801 cgaggtcaca acatagtaaa agcgctattg gtaatggtac aatcgcgcgt ttacacttat
     4861 tcagaacgat tttttttcag gagacacgaa catggccagc agaggcgtaa acaaggttat
     4921 tctcgttggt aatctgggtc aggacccgga agtacgctac atgccaaatg gtggcgcagt
     4981 tgccaacatt acgctggcta cttccgaatc ctggcgtgat aaagcgaccg gcgagatgaa
     5041 agagcagact gaatggcacc gcgttgtgct gttcggcaaa ctggcagaag tggccagcga
     5101 atatctgcgt aaaggttctc aggtttatat cgaaggtcag ctgcgtaccc gtaaatggac
     5161 cgatcaatcc ggtcaggatc gctacaccac agaagtcgtg gtgaacgttg gcggcaccat
     5221 gcagatgctg ggtggtcgtc agggtggtgg cgctcctgca ggtggcaata tcggtggtgg
     5281 tcagccgcag ggcggttggg gtcagcctca gcagccgcag ggtggcaatc agttcagcgg
     5341 cggcgcgcag tctcgcccgc agcagtccgc tccggcagcg ccgtctaacg agccgccgat
     5401 ggactttgat gacgatattc cgttctgatt tgtcattaaa acaataggtt atattgtttt
     5461 aaggtggatg attaaagcat ctgccagcca taaaaaagaa gcctccgtta tggaggcttc
     5521 tacgtatcag gtcaaaatca ttggccactg tggcggtgtc tgattcatct cttctatcat
     5581 caccgactcg atgttgtgcc agatggcggt gatgtccgcc attgtgatgc caagcaaccc
     5641 cattgcgaac cagcaggcgg aaaccacgcc cagaccgctg ctgatcactg caagcccgac
     5701 ataatcagac cgacgaaaac ggatgttgag cgtgctggcc agaaacatca ttacggcact
     5761 aagaagttgc cagcgaagaa gaaccacgcc agtggtgagg gtagccatca aacaattcct
     5821 ctgaaaagag ccgatgccct ggacagcgcg gcgttgttca cgggaggcgt ggtacactct
     5881 ggctatcgcg gggcttgcag aacacaaaaa tgaaacactc tgtttgtttc attaattttg
     5941 tgaactatat cacaattgat tgtttgttag ccagattagg tcgtgacttt tattgctgta
     6001 cagattatgt ggtttttcag tggcattaag ggtatatctt cccgccgcct ctgtattcct
     6061 gtagaaaatt aattttgaat atcaatgaat tattttcatc caagtgacga ttaaaaaggt
     6121 atcaatttca aatcaggcaa aagtgctatt tataccgtaa gatttatcta aagacgtcgg
     6181 tagccagggt ttcaccttgc aatggccggg tataaacagg caggaaattg atagcaatga
     6241 atcatcgtgc acgacaccaa ttactggcgt tgccgggcat tatcttttta gttctctttc
     6301 ccatcattct ttcgctatgg attgccttcc tttgggcaaa atcagaagtg aataatcagc
     6361 tccgaacctt tgctcaactg gcgttggata aatccgagct ggtcattcgc caggcagatt
     6421 tagtgagcga tgcagctgaa cgctatcagg ggcaagtttg cactccagcc catcaaaagc
     6481 gaatgttgaa tattattcgt ggctatcttt atattaatga attgatctat gcccgtgata
     6541 atcatttttt atgctcatcg ctgatagtgc ctgtaaacgg ctatacgatt gtatcggccg
     6601 attataagcg tgaacctaat gtttctatct attattaccg cgatacgcct tttttctctg
     6661 gctataaaat gacctatatg cagcggggaa attatgtggc ggttatcaac cctctcttct
     6721 ggagtgaagt gatgtctgat gacccgacat tgcaatgggg tgtgtatgat acggtgacga
     6781 aaaccttttt ctcgttaagc aaagaggcct cagcagcaac gttttctccg ctgattcatt
     6841 tgaaggattt aaccgtacca agaaatggct atttatatgc gacagtttat tcgacaaaac
     6901 gcccaattgc agccattgtt gcgacttcat atcaacgtct tatagcccat ttttataatc
     6961 atcttatttt tgcgttgccc gccggtattt tggggagtct tgttctgcta ttactctggc
     7021 tacgtattcg acaaaactat ttgtctccca aacgcaaatt gcaacgcgcc ctcgaaaaac
     7081 atcagctatg cctttattac cagccaataa ttgatatcaa aacagaaaaa tgtatcggcg
     7141 ctgaagcgtt gttacgttgg cctggtgagc aggggcaagt aatgaatccg gcagagttta
     7201 ttcctctggc agaaaaggag gggatgatag aacaggtaac tgattatgtt attgataatg
     7261 tcttccgcga tctgggcgcg taccttgcaa cacatgctga tcgctatgtt tctattaatc
     7321 tgtcggcctc cgattttcat acgtcacggt tgatagcgcg aatcaatcag aaaacagagc
     7381 aatacgcggt gcgtccgcag caaattaaat ttgaagtgac tgagcatgca tttcttgatg
     7441 tcgacaaaat gacgccgatt attctggctt tccgccaggc tggttacgaa gtggcaattg
     7501 atgattttgg tattggctac tctaacttgc ataaccttaa atcattgaat gtcgatattt
     7561 tgaaaatcga caaatcgttt gttgagacgc tgaccaccca taaaaccagt catttgattg
     7621 ctgaacatat catcgagctg gcgcacagcc tggggttaaa aacgatcgct gaaggcgtcg
     7681 aaactgagga gcaggttaac tggctgcgca aacgcggcgt gcgctattgc cagggatggt
     7741 tctttgcgaa ggcgatgccg ccgcaggtgt ttatgcaatg gatggagcaa ttacccgcgc
     7801 gggagttaac gcgcgggcaa taaaactaca ggcggtggcg ataatcgctg ggagtgcgat
     7861 caaactgccg acggaaaacg cgggagaagg tctgctgaga gacataaccc aggtccattg
     7921 cgatatcaaa aatcggacgc tcggtggtgc gcaactcaac ggcggccagt aacagacggc
     7981 gttgacgaat gtaatcgcca agcgtctgat gcgtcaccgt gcggaacatc cgttgcaggt
     8041 accactttga atagcctgat ttttttgcga cgacatcaat gttaagcggc tggtcaatat
     8101 gctcgtcaat ccatgcgata agatcctgaa taattttctg atgggacata aatctgcctc
     8161 ttttcagtgt tcagttcgtt aattcatctg ttggggagta taattcctca agttaacttg
     8221 aggtaaagcg atttatggaa aagaaattac cccgcattaa agcgctgcta acccccggcg
     8281 aagtggcgaa acgcagcggt gtggcggtat cggcgctgca tttctatgaa agcaaagggt
     8341 tgattaccag tatccgtaac agcggcaatc agcggcgtta taaacgtgat gtgttgcgat
     8401 atgtcgcaat tatcaaaatt gctcagcgta ttggcattcc tctcgcgacc attggtgaag
     8461 cgtttggcgt gttgcccgaa gggcatacgt taagtgcgaa agagtggaaa cagctttcgt
     8521 cccaatggcg agaagagttg gatcggcgca ttcatacctt agtggcgctg cgtgacgaac
     8581 tggacggatg tattggttgt ggctgccttt cgcgcagtga ttgcccgttg cgtaacccgg
     8641 gcgaccgctt aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa caaaactaaa
     8701 gcgccacaag ggcgctttag tttgttttcc ggtctttgtc tttctctcta tcccgctggt
     8761 acacaggagg gtttcccccg acgtcaacac acctcattcg agcacgtggt ggaggttccg
     8821 gttggtgttg atgctttaat tgtatgtcac cgacgtttct tcgccagtgt aaaagtatac
     8881 tttttagccg caatattttt gtcatctcag acgatttttt atcgcaatcc tcaacggtat
     8941 acggctcgat aacgctgcaa tcttgcgcat cgacgataac gtttgcgcat caattgcctg
     9001 gtttttcatc gtcaagacaa taaaagagaa aaaagcagca aacttcggtt gaaaaagccg
     9061 ctatgatcgc cggataatcg tttgcttttt ttaccacccg ttttgtatgc gcggagctaa
     9121 acgtttgctt ttttgcgacg cagcaaattg tcgcaaacct ggagcaggaa gataacgttt
     9181 cgctggcagg ggattgtccg ccacgcatct tgacgaaaat taaactctca ggggatgttc
     9241 tcttatgtct acgccatcag cgcgtaccgg cggttcactc gacgcctggt ttaaaatttc
     9301 acaacgtgga agcactgtcc gtcaggaagt ggttgccggg ttaacaacgt ttctggcgat
     9361 ggtctactcg gttatcgtcg ttccaggcat gttgggtaaa gcgggcttcc cgcctgcggc
     9421 agttttcgtt gcgacctgtc tggttgccgg actcggttct atcgtgatgg gtctgtgggc
     9481 taatctgccg ttggcgattg gttgcgccat ctccctgacg gcgtttaccg cattcagcct
     9541 ggtgctgggg caacatatta gcgtacctgt cgcgctgggt gccgtgttcc tgatgggtgt
     9601 gctgtttacg gtaatttctg ccacgggtat ccgtagctgg attttgcgca acttgccgca
     9661 cggtgtggcg cacggcacgg ggatcggtat cggtctgttc ctgctgctca ttgccgctaa
     9721 cggtgtcggt ctggtgatta aaaacccgct tgatggtctg cccgttgcgc tgggtgattt
     9781 cgcgaccttc ccggtgatta tgtcactggt aggtctggcg gtgatcatcg gcctggaaaa
     9841 actgaaagtc cctggtggca ttctgctgac cattatcggt atctcaattg tcggtttgat
     9901 cttcgatcct aacgtccatt tctccggcgt tttcgccatg ccttcattga gcgatgaaaa
     9961 cggcaattca ctgattggca gcctggacat tatgggcgcg ctgaatcctg tagtcctgcc
    10021 aagcgttctg gcgctggtga tgacggcagt atttgatgcc accggaacta tccgcgctgt
    10081 cgccggccag gcgaacctgc tggataaaga tgggcagatc atcgacggcg ggaaagcact
    10141 gaccactgac tccatgagca gcgttttctc tggcctggtg ggtgcagctc cggcagcggt
    10201 atacatcgag tctgcggcgg gtacggcggc gggcggtaaa accggtttga cggctatcac
    10261 cgttggcgtg ctgttcctgc tgattctgtt cctctctccg ctttcttacc tcgttccggg
    10321 gtatgcaacg gctccggcgc tgatgtacgt tggcctgctg atgctgagca acgtggcgaa
    10381 aatcgacttt gctgattttg ttgatgcgat ggcgggtctg gttacggcgg tattcatcgt
    10441 gctgacctgt aacatcgtaa caggcatcat gatcggcttc gcgactctgg tgattggtcg
    10501 tctggtttcc ggtgaatggc gcaagttgaa catcggtacg gtcgttatcg ccgtggcgct
    10561 ggtgaccttc tatgcgggtg gctgggctat ctaatctttc atcggattct gaaaacgggt
    10621 ggcaatggct gcccgttttt attttctccg cacattgtgt gagcttttgc gatattctga
    10681 aaaaatgaga attcaggcat aacgtctttc tcagatcata aaaaacatcg caaacaggga
    10741 acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcgc tgtccggggt
    10801 ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg ccatcggtgc
    10861 gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac tctttttagt
    10921 cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc gtgaatttct
    10981 cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca ccgtggtcgg
    11041 cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg cctttgcgct
    11101 ggcggcggtg ctttctccga ccgatgctgt ggcgctctcc gggattgttg gcgaagggcg
    11161 catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg acgcctccgg
    11221 cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct tcaccgtcgg
    11281 cggtgcgacg gtcgaattta tgaaagtagc cattggcggt attctcgccg gttttgtggt
    11341 gagctggctg tacggtcgct cgctgcgatt cctcagccgc tggggcggtg atgaacccgc
    11401 gacgcagatc gtcctgctgt tcctgctgcc attcgcttct tatctgattg ccgaacatat
    11461 tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc gctccggtgt
    11521 gatgcgccgt gcgccgctgg caatgcgcct gcgtgcaaac agcacctggg cgatgctgga
    11581 atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg gtattctgga
    11641 gacgtcgctg atggcagcag aaatcgaccc taacgtcgaa atctggatgc tgtttaccga
    11701 tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt ggacgatgaa
    11761 aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt ggaccacacg
    11821 agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactttgg ccggtgtgct
    11881 ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg agctggtgtt
    11941 tctggcggct ggtgtcattc tcttctcgct gtttgtcggc gtggtgatgt tgcctattct
    12001 gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac gtattgcgcg
    12061 agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc gtctggcggc
    12121 ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc gcgtcattgg
    12181 taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc aggaagagaa
    12241 ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac tgtaccacct
    12301 gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg atctcgattt
    12361 gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa ccaggcccga
    12421 ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg gctaacattc
    12481 atgattctaa aacaaataag ttatctctct tttaaagtca tttcattggc tgttcttcga
    12541 cttaatctca atctgcgtgt tgcataatat ctgcagtaat cttcatagct acttcatgag
    12601 tattattctc agtttgtgac atgccagtat gagaggtaca atcttcttca aagaatttct
    12661 gtagtgttac cagggtatca ctattaatgg gaatattctc ctccccacgg agaatggaaa
    12721 tgcccattcc ctggacgcta gcctcatcga aattaacccg atagagacgg gtattaaaca
    12781 tccaggcttt tgttaaaaca gaacagttga aatggcaatt gtttaaattg gcattattaa
    12841 aattaacatt agccatatta gaatcggtgc atataaccat ttcgaggaac gtatttgtta
    12901 gccgtgtaaa aaagagttta ctatcaatta agtttatatt ctggaggatt gctttgttca
    12961 gattgacgcg agacagatca gcatgtttaa agttaattaa atcaagttta gagaagctaa
    13021 ggtcggctct aaataaatt
//
LOCUS       DAZFBR010000122        12959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFBR010000122 DAZFBR010000000
VERSION     DAZFBR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.122"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..238
                     /locus_tag="ACHJZ2_003988"
     CDS             <1..238
                     /locus_tag="ACHJZ2_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO4570013.1"
                     /translation="IEEETIFSDAELKAILPMRRMYGVSDYKSNSSSLSSHSGLKDPT
                     GTPVCYYIHNESKPSLGYGPTSNNWLSQSFTTEL"
     gene            301..1560
                     /gene="espR1"
                     /locus_tag="ACHJZ2_003989"
     CDS             301..1560
                     /gene="espR1"
                     /locus_tag="ACHJZ2_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HFO4570014.1"
                     /translation="MKFPSIFNKIKPQSIQQHPEKNQLNWMLELNKWKEERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLSTQPPGLQNFTSINLDNNQLTHFDATNY
                     DRLVKLSLNSNTLESINIHQGRNVSITHISMNNNCLRNIDIDRLSSITYFSAAHNKLE
                     FVQLESCEWLQYLNLSHNQLTDIVTGNKEELLLLDLSHNKLASLHNALFPNLNTLLIN
                     NNLLSEIKMFYSNFCKVQTLNAANNQLEKINLHFLTYLSSIKSLRLDNNKITRIDTEN
                     TSDIRSLFPIIKKSESLNFLNISGENNCPTIQLMLFNLFSPALKLNTGLAILSPGAFE
                     DHSDGLDVDNELFHYTINKAYTPYNIHTYKTEEVVNQRNIKIKNMTLDEINNTYCNND
                     YYNEAIREEPIDFLDRSFSSSSWPFYH"
     gene            1866..3254
                     /gene="narU"
                     /locus_tag="ACHJZ2_003990"
     CDS             1866..3254
                     /gene="narU"
                     /locus_tag="ACHJZ2_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO4570015.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            3336..7076
                     /gene="narZ"
                     /locus_tag="ACHJZ2_003991"
     CDS             3336..7076
                     /gene="narZ"
                     /locus_tag="ACHJZ2_003991"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO4570016.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRICPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            7073..8617
                     /gene="narH"
                     /locus_tag="ACHJZ2_003992"
     CDS             7073..8617
                     /gene="narH"
                     /locus_tag="ACHJZ2_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO4570017.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            8617..9312
                     /gene="narW"
                     /locus_tag="ACHJZ2_003993"
     CDS             8617..9312
                     /gene="narW"
                     /locus_tag="ACHJZ2_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO4570018.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            9309..9989
                     /gene="narI"
                     /locus_tag="ACHJZ2_003994"
     CDS             9309..9989
                     /gene="narI"
                     /locus_tag="ACHJZ2_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO4570019.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            10068..10961
                     /gene="yddE"
                     /locus_tag="ACHJZ2_003995"
     CDS             10068..10961
                     /gene="yddE"
                     /locus_tag="ACHJZ2_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO4570020.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(11057..11901)
                     /gene="nhoA"
                     /locus_tag="ACHJZ2_003996"
                     /pseudo
     CDS             complement(11057..11901)
                     /gene="nhoA"
                     /locus_tag="ACHJZ2_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            12200..12643
                     /locus_tag="ACHJZ2_003997"
     CDS             12200..12643
                     /locus_tag="ACHJZ2_003997"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO4570021.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(12647..12874)
                     /gene="pptA"
                     /locus_tag="ACHJZ2_003998"
     CDS             complement(12647..12874)
                     /gene="pptA"
                     /locus_tag="ACHJZ2_003998"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO4570022.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
BASE COUNT         3255 a         3258 c         3236 g         3210 t
ORIGIN      
        1 aatagaagaa gaaacaatat tttctgacgc agaactaaaa gctattctgc ctatgcgtcg
       61 catgtacggg gttagtgact ataagtcaaa ttcctcttct ctatcctcac actcgggact
      121 caaggaccca acgggcacac ccgtctgtta ttatattcat aatgagagta aaccttcctt
      181 aggttatggt ccaacatcca ataattggtt aagccaatcc tttacaacag agttataaaa
      241 cacatcctgg tttatcaagg gtgataaatc agaaaaacat tgtattacta tggagcagtt
      301 atgaaattcc cttcaatatt taacaaaata aaaccacaat ccatacagca acatccagaa
      361 aaaaatcaac ttaactggat gctcgaatta aataaatgga aagaagaacg tatacttaca
      421 ggtgaaatcc atcgtccgga atgtcgaaac gaagccgcta aaaggataaa ctgtgctttt
      481 ttgtcgaaac agaatgacat tgatttatca ggacttaatt tatctactca accaccaggg
      541 ctgcaaaact tcacctctat caatcttgat aataaccaac tcacacattt tgatgcaacc
      601 aactacgata gactcgtaaa acttagtctg aatagtaaca ctcttgagtc aataaatatt
      661 catcaaggca gaaatgtaag cattacacat atatctatga ataataattg tctcagaaat
      721 attgatatag ataggctttc atcaattact tattttagtg cggcacataa taaactagag
      781 tttgtgcaat tagaatcttg cgaatggctg caatacctga atctcagcca taatcaatta
      841 actgatattg ttacaggaaa taaagaagaa ctcttactgc tggatctatc ccataataaa
      901 ctagcaagtt tacacaatgc cttatttccc aacttaaata cgttacttat caacaacaac
      961 ttgctttctg aaattaaaat gttttatagc aacttctgca aagttcagac attaaacgct
     1021 gctaacaatc agttggaaaa aataaacctt catttcctga cttatctttc atctatcaaa
     1081 agtttaaggc tggacaataa taaaataact cgcattgata ctgagaacac atccgatatt
     1141 agaagtttat tccccataat aaagaagagc gaaagcttaa attttttaaa tatttctggc
     1201 gagaacaatt gccctactat ccagctcatg ttatttaatt tgttttcccc agcacttaag
     1261 cttaatactg gcctggcaat tctttcgcct ggtgcatttg aagatcactc tgacggatta
     1321 gatgtggata acgaattgtt tcactatact attaataaag catatacccc atataatata
     1381 catacttata aaacagaaga agttgtaaac cagaggaata taaaaattaa aaatatgacc
     1441 ttagatgaaa taaacaatac ttattgtaat aacgattatt acaatgaggc aataagagag
     1501 gaaccgatag actttctgga cagatcgttt tcctccagct catggccttt ttatcactaa
     1561 ttctataagt gtctggtctt gttaaaaata tagttaatat acaacccttt ggattcatca
     1621 tctgaagggt tgaatcatga catgcgtaca tagcttaacc ccgtttaatg gttagcttgt
     1681 tagttacatt tagtaacaca taactggctg cttaatcccc attctgcccc cgcggttacg
     1741 ctttcttatc tattctttca atcagcactt cacccatttc atccttaatt ctaataactg
     1801 gctattgcgc cttacctcct gttttgtctt tttttcatta ccaatgtgta catgtgagga
     1861 acaatatggc actgcaaaat gagaaaaata gtcgttatct tttacgcgac tggaaaccag
     1921 aaaatccggc cttctggaaa aataaaggaa aacatattgc tcgaagaaac ctctggatat
     1981 cagtcagttg tctacttctt gccttctgtg tctggatgct atttagcgca gttaccgtta
     2041 atctcaataa aatcggtttt aatttcacta ccgatcaact ctttttatta accgcattac
     2101 cctccgtttc tggcgcatta ttacgtgtcc cctactcctt tatggtgcct atattcggtg
     2161 gacgtcgatg gacggttttt agtactgcaa tcctgattat tccttgcgtc tggctcggaa
     2221 ttgccgtgca gaatccgaat actccttttg ggatatttat cgttatcgct ttgctatgcg
     2281 gttttgcagg tgcgaacttt gcttcgagca tgggcaatat cagtttcttc tttccaaaag
     2341 ccaggcaagg tagcgctctt gggattaatg gcggattagg aaacttaggt gtaagtgtaa
     2401 tgcagctggt tgcaccgctg gtcatttttg tacccgtatt tgcctttctc ggcgtcaatg
     2461 gagtaccgca ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg atttgggtac
     2521 cgctactagc gattgccacg atcgccgcct ggtcagggat gaatgatatc gccagttcac
     2581 gcgcgtcaat tgccgaccag ctccctgtct tacaacgcct gcatctctgg ctgctgagcc
     2641 tgctttacct tgccaccttc ggttcgttta tcggtttttc tgcgggtttc gccatgctgg
     2701 caaaaaccca gttcccggat gtgaatattc tgcgcctggc gttctttggc ccatttatcg
     2761 gtgccatcgc gcggtcggtg ggtggtgcta tttccgataa attcggcggc gtgcgggtga
     2821 cgttgatcaa ctttattttt atggcgattt tcagcgccct gctgttcctt accttaccgg
     2881 gcacaggctc cggtaacttc atcgcctttt acgccgtatt tatggggctg tttctgaccg
     2941 cgggtctggg aagtggttct actttccaga tgatcgccgt catctttcgc cagataacca
     3001 tttatcggat gaagatgaaa ggcggtagtg atgagcaagc tcagagagaa gccgtcaccg
     3061 aaacggcggc ggctctgggc tttatctcag ccatcggcgc agtgggcggc ttttttattc
     3121 cgcaggcatt tggcatgtca ctcaatatga ccggctctcc agtgggcgcg atgaaagtgt
     3181 ttttaatctt ctacatcgtt tgtgtgctac tgacctggct ggtttatggt cggcggaagt
     3241 tcagccaaaa ataagagatc gatgcctgat gtacaaccgt gtcgcatccg acaaggcatc
     3301 agatcccaat tcattcctgg agcaggagtc atgtcatgag taaacttttg gatcgctttc
     3361 gctacttcaa acaaaagggc gatacctttg ccgatggtca cggacaggtg atgcatagca
     3421 accgcgactg ggaggacagc tatcgccagc gttggcagtt cgacaaaatc gtgcgttcca
     3481 cccacggtgt taactgtaca ggctcctgta gctggaaaat ctacgttaaa aatggtctgg
     3541 tgacctggga aatccaacag accgactacc cgcgcactcg ccctgacctg cccaatcatg
     3601 aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta tctttacagc gctaaccgcc
     3661 tgaaataccc gctcattcgt aaacgactga ttgaactgtg gcgcgaagcc ctcaaacaac
     3721 acagcgatcc ggtactggcg tgggcatcga ttatgaacga tccgcaaaag agcctgagct
     3781 acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa ctggccggaa ctaaaccagc
     3841 tgattgccgc cgctaacgtc tggactatca aaacctacgg cccggatcgc gttgccggtt
     3901 tctcgccgat cccggcgatg tcgatggttt cttacgccgc cggaacacgt tatctgtcac
     3961 tattaggtgg cacctgttta agcttctacg actggtattg cgacctgccg cccgcctcgc
     4021 cgatgacctg gggcgagcaa accgacgtac cggaatctgc cgactggtat aactccagtt
     4081 acatcatcgc gtgggggtct aacgtaccgc agacacgtac gccggacgcc catttcttta
     4141 ccgaagtacg ctacaaaggc actaaaacca tcgccattac ccctgactac tctgaagtgg
     4201 ccaaattgtg cgaccagtgg ctggcaccaa aacaaggcac tgatagcgcc ctggcgatgg
     4261 caatgggcca tgtgatttta aaagagtttc atctcgataa tcccagcgac tactttatca
     4321 actactgccg ccgctatagc gacatgccga tgctggtaat gctggaacct cgcgacgatg
     4381 gtagctacgt acccgggcgc atgatccgcg cgtctgacct ggtagatgga ctaggcgaaa
     4441 gcaataatcc gcagtggaaa accgtggcgg ttaataccgc aggtgaattg gtagtgccga
     4501 atggttcgat tggtttccgc tggggagaaa aaggcaaatg gaatctggaa tccattgccg
     4561 ccggtaagga aaccgaattg tcgttaaccc tgctcggtca acatgacgcc gttgcaggcg
     4621 tggctttccc ctactttggc ggcatcgaaa atccgcattt tcgcagcgtc aaacacaatc
     4681 ctgtcctggt gcgtcaatta cccgttaaaa acctgacatt agccggtggc agcacctgtc
     4741 cagtggtcag cgtttatgat ttggtactgg cgaattacgg cctcgatcgc gggctggaag
     4801 atgaaaacag cgcgaaagat tacgctgaaa tcaaaccgta caccccagcc tggggtgagc
     4861 aaattaccgg cgtgccgcgc cagtatattg aaaccatcgc tcgtgaattt gccgatactg
     4921 cccataaaac gcatgggcgc tcgatgatta tcctcggcgc aggtgttaac cactggtatc
     4981 acatggacat gaactaccgg ggaatgatca atatgctgat cttctgcggt tgtgtcggac
     5041 aaagcggtgg cggctgggca cactatgtcg gtcaggaaaa actgcgcccg caaaccggct
     5101 ggttgccgct ggcctttgcg ctcgactgga accgaccacc gcgccagatg aacagcacct
     5161 cgtttttcta caatcattcc agccaatggc gctatgaaaa agtctctgcg caggagttac
     5221 tttcaccgct tgccgatgcc agtaagtaca gcggtcatct gattgatttt aacgttcgcg
     5281 ccgaacgcat gggctggcta ccttctgcgc cgcagttggg gcgtaacccg ctcgggatta
     5341 aagctgaagc cgacaaggcc ggattatccc ccacagaatt taccgcccag gcgctgaaat
     5401 cgggcgattt acgtatggcc tgcgaacaac cagatagcgg cagcaatcat ccgcgtaatt
     5461 tgtttgtctg gcgttctaac ctgcttggct cctccggcaa aggccacgag tatatgcaga
     5521 agtatctgct ggggaccgaa agcgggattc agggcgagga actcggtgcc agcgacggga
     5581 tcaaaccgga agaagtcgag tggcaaactg cggcgattga aggcaagctc gacctgctgg
     5641 tgacgctcga cttccgcatg tccagtacct gcctgttctc cgatatcgtt ctgcccaccg
     5701 ccacctggta tgaaaaagac gatatgaaca cctcggatat gcatccattt attcatccgc
     5761 tttctgcggc ggtcgatccg gcctgggaat cacgcagcga ctgggaaatc tacaaaggta
     5821 ttgccaaagc attttcgcaa gtgtgcgtgg gccatcttgg caaagaaacc gacgtggtat
     5881 tacaaccact gctgcatgac tctccggcag agctctcaca gccgtgtgaa gtgctcgact
     5941 ggcgcaaagg cgaatgcgat ctgagcccgg gtaaaaccgc gccgaatatt gtggcggtgg
     6001 agcgcgacta ccctgctacg tatgaacgct ttacctcgct cgggccattg atggacaaac
     6061 ttggcaacgg cggtaaaggg atttcgtgga atacgcagga tgaaatcgat ttcctcggta
     6121 aactcaatta caccaagcgt gatggcccag cgcaggggcg tccgctgatt gacaccgcca
     6181 ttgacgcttc agaagtgatt ctggcactgg caccagaaac caacggtcat gttgcagtta
     6241 aagcgtggca ggcgctgggc gagatcaccg gacgcgaaca tacccatctg gcgctgcaca
     6301 aagaggacga gaagattcgc tttcgcgata ttcaggcgca gccgcgtaaa attatctcca
     6361 gccccacatg gtctggtctg gaaagcgatc acgtctccta taatgcggga tacaccaacg
     6421 ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca gcagctctat caggatcatc
     6481 cgtggatgcg tgcttttggt gaaagcctgg tggcatatcg cccgcctatc gacacccgta
     6541 gcgtcagtga gatgcgccag atcccgccaa acggcttccc ggaaaaagca cttaacttcc
     6601 tgacgccgca ccagaaatgg ggcattcact caacctacag tgaaaacctg ctaatgctga
     6661 cgctctctcg cggtggaccg attgtctgga tcagcgaaac cgatgcccgt gaactaacca
     6721 ttgtcgataa cgactgggtg gaagtgttta acgccaatgg cgcgctgacg gcccgcgcgg
     6781 tggtcagcca acgtgtaccg ccgggtatga ccatgatgta tcacgctcag gaacgcatta
     6841 tgaatattcc tggttcggaa gtaactggca tgcgcggcgg tattcataac tcggtcaccc
     6901 gcatttgccc gaaaccaacg catatgattg gcggttacgc gcagctggcc tggggcttta
     6961 actactacgg caccgtcggc tcgaaccgcg acgaattcat catgatccgc aagatgaaga
     7021 acgttaactg gctggatgat gaaggtcgcg atcaggtaca ggaggcgaaa aaatgaaaat
     7081 ccgttcgcaa gtcggcatgg tgcttaatct cgataaatgt atcggctgcc atacctgttc
     7141 ggtgacctgt aaaaacgtct ggaccggacg cgaaggcatg gagtacgcat ggtttaacaa
     7201 cgtcgaaacc aaaccgggca ttggttatcc gaaaaactgg gaagatcagg aagagtggca
     7261 aggcggctgg gtccgcgatg tgaatggcaa gatacgcccg cgtctgggca gcaagatggg
     7321 tgtaataacc aaaatcttcg ccaacccggt ggtgccgcag attgatgatt actacgaacc
     7381 tttcaccttc gactacgaac atttgcatag cgcaccggaa ggcaaacata tccctactgc
     7441 tcgcccgcgt tcgctgattg acggtaagcg gatggacaaa gtgatctggg ggccaaactg
     7501 ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc gaccgcaact tcgaggccat
     7561 gcaaaaggag atgtacgggc agtttgaaaa caccttcatg atgtacctgc cgcgcctgtg
     7621 cgaacactgc ctcaatccca gctgcgtggc gacctgccca agcggcgcta tctacaaacg
     7681 cgaagaagac ggcattgtgc tgattgatca ggataaatgc cgtggctggc gtttgtgcat
     7741 tagcggttgt ccgtacaaaa aaatctactt caactggaaa agcggcaagt cagaaaaatg
     7801 tatcttctgt tacccacgaa ttgagtccgg tcaaccgacc gtgtgctcag aaacctgcgt
     7861 gggtcgcatc cgatatctgg gcgtgctgct ttacgacgct gaccgcatcg aggaagcggc
     7921 gagcaccgag cgcgaggttg acctctatga acgccagtgc gaagtgttcc tcgatccgca
     7981 cgatccctca gtgatcgagg aagccctgaa acaaggtatt ccacaaaatg tgattgacgc
     8041 tgcccagcgt tcgccagtct acaaaatggc gatggactgg aaactggcac taccgctgca
     8101 ccctgaatac cgcaccctgc cgatggtctg gtacgttcct ccgctgtcac cgattcagtc
     8161 ctacgcagat gcgggcggtt tgccgaaaag cgaaggcgtg ctgcccgcca tcgaaagcct
     8221 gcgtattccg gtgcaatatc tcgccaatat gttgagtgcc ggcgataccg gtccggtact
     8281 gcgggcgctg aaacggatga tggcgatgcg ccactatatg cgttcacaaa ccgtggaagg
     8341 cgttactgat actcgtgcca tcgacgaagt aggcctgagc atcgcccagg tcgaagaaat
     8401 gtatcgctat ctcgccattg ccaactatga agatcgtttt gttatcccga ccagccatcg
     8461 ggaaatggcg ggcgatgcct tcgcagaacg caatggctgc ggttttacct ttggtgacgg
     8521 ttgtcacggc tcggacagca aattcaacct gttcaacagt agccgtatcg atgccatcaa
     8581 catcaccgaa gtgcgcgaca aagcggaggg cgaataatgc agatcctcaa agtgatcggc
     8641 ctgttgatgg agtatccgga cgagatgttg tgggaatgca aggaggacgc gctggcgttg
     8701 atccgccgcg atgcgccgat gcttacggat ttcacccgca acctgcttaa cgcgccgctg
     8761 ctggataaac aggccgaatg gtgcgaagtg tttgaccgcg ggcgcaccac gtcgctgctg
     8821 ctgttcgaac atgttcatgc cgagtcccgc gatcgcggcc aggcaatggt ggacctgctg
     8881 gcggagtatg aaaaggtcgg cctgcagctg gattgtcggg aactgcccga ttatctaccg
     8941 ctgtatctgg agtatttaag cgtactgcct gacgatcagg cgaaagaagg attgctcaac
     9001 gttgcgccga tcctcgccct gcttggcggt cgcttaaaac aacgcgaggc accgtggtac
     9061 gcgttgtttg atgctctgct gcaactggcg ggaagtacac tttcaagtga cagtgtcaca
     9121 aaacaagtaa acagcgaaga gcgcgatgac acccgccagg cgcttgatgc agtgtgggaa
     9181 gaggaacagg tgaagtttat tgaagataac gccacggcgt gtgacagctc gccgcttaat
     9241 caatatcagc gacgctttag tcaggatgtc gcgccgcaat atgtcgacat cagtgcggga
     9301 ggtgggaaat gattcagtat ctgaacgtct ttttttacga tatctacccg tacatttgtg
     9361 cgacggtgtt tttcctcggc agctggctgc gctacgacta cgggcagtac acctggcgcg
     9421 cctcctcaag tcagatgctc gataaacgcg ggatggtgat atggtcgaat ctgttccata
     9481 tcggcatttt ggggatcttc ttcgggcacc tgttcggcat gttaacgcca cactggatgt
     9541 acgcgtggtt tttgccagtg gcagcgaaac aactaatggc gatggtgctc ggtggtattt
     9601 gcggtgtgtt gacgttgatt ggcggcgcag gactgctgtg gcgcaggctg acaaatcagc
     9661 gcgtgcgtgc cacttccact acgccggata tcatcatcat gagcattttg ctgatccagt
     9721 gtctgttggg gctaagtacc ataccgttct cagcacagta tcctgatggt agcgaaatga
     9781 tgaagctggt gggttgggcg caaagcattg tcactttccg tggtggatcg tcagagatgc
     9841 ttaacggtgt agcgttcgtc ttccgcctgc atctggtgtt gggaatgacc atcttcttgc
     9901 tcttcccatt cacccgactg gtgcatgtgt ggagcgcccc gtttgagtac tttactcgtc
     9961 ggtatcagat tgtgcgctcg cggcgataat ttcaccctgg ccggatagcc cttcgctctc
    10021 cggccacttg caggctatga tagttctcca gtcacaggag aacgttaatg aaaccgcagg
    10081 tttaccacgt cgatgctttt acctctcaac catttcgcgg caattctgcc ggtgttgttt
    10141 tccccgccga taatcttagc gaagcgcaaa tgcagcttat cgcccgcgaa ttaggtcatt
    10201 cggaaaccgc ttttctgctg cacagcgacg acagcgatgt gcgcatccgc tactttacgc
    10261 caacagttga agtgcctatt tgcggtcacg cgacagtagc tgcgcactat gtacgtgcga
    10321 aggtgttagg tttaggaaat tgcacggtct ggcaaacatc gctggcagga aaacatcgcg
    10381 tgactatcga aaagcaccac gatggttatc gtatttcgct ggaacaaggt acgccaggct
    10441 ttgagccacc gctggaaggt gaaacacgtg cggcgattat caacgcgctt catctcactg
    10501 aggacgatat tctgccaggc ttgccaattc aggtggcaac cacaggacac tcaaaagtga
    10561 tgatcccact gaaaccggaa gtggatatcg acgccctttc gcctgacctt aatgcgctga
    10621 ccgctatcag caaacagatt ggctgcaatg gtttcttccc gttccagatc cgcccaggca
    10681 aaaacgaaac cgatggtcgc atgttctcgc ctgcgattgg tattgtggaa gatccggtga
    10741 ctggcaatgc caatggcccg atgggtgcat ggttggtaca tcacaacgta ttgccccacg
    10801 atggcaacgt gttgcgtgtt aaaggccatc aggggcgcgc attggggcgt gacggtatga
    10861 ttgaagtgac ggtgacaatt cgtgataacc aaccggaaaa agtcaccatt tctggcgcgg
    10921 cggtgatttt gtttcatgcc gaatgggcaa ttaaactctg aaaccgttgc ctgatgcgct
    10981 tcgcctgtca ggtctacaac cgggctaatg tatgccggat aaggcgttta cgccgcatcc
    11041 ggcaacctga cataaattat tttcccgcct ccgggtgcgt atcaaacgcc gccatcacca
    11101 gcgctaactc atccacggta aagccatgtt tcgcatcatc cacgcccaga ccaaactgtt
    11161 cttgcatcac agcatataac gacgccacat ccggtagatt tcgctgctcc accgcgtgcc
    11221 cattttcata atgggaaaat gaaaattggt cagtgtcagc ttgccgccgt ccggcaaatg
    11281 gcggcacatc agcaaatgat ggcgaaaatg cgactgcggc cagtgcgccg accagaaatt
    11341 acccatcaca taatcgcttt gttgctgctc gcagagatca aaacggtaca tcgactgcca
    11401 gtgctgatga tgattaaact gcaacaccca atcatcaccc tcctgcaaca accgatactc
    11461 tccgtgtggc gtggtctgca caagatcggg aactaaacga atcggcgcgg ttagcgtctg
    11521 cccaccgaaa ccgacatcag caatccattt ttcctcttcc agttccacca acagcaaacg
    11581 atgggtgcgc ggcggtaatg ctggcggatt tgataacact acccgcccta acaagctgcg
    11641 aacgttaaac cccagctcgc gtaacacccg ctcaaaaacg ccattctgct caaaacagta
    11701 accgccacga cgggctatca ccagtttctc ttccagcgat tgatcatcaa gctgcatttc
    11761 cctcggcagc aaaacgtcga ggttttcaaa cggaatggtg caattgtgtt tcaggtgcaa
    11821 tgcacgaagc gtatcaatat tgaccgcagc agctcccgac cagttaatac gggcaaaata
    11881 gtgattcaga atgggcgtca tcaggcgttt cccttagtgg ttttctcaac ttataacctg
    11941 gaatagacgc cgcaccggct attttgtgcg atcgcggaca tgcatcacgt tcgcaaatcg
    12001 ccaatcccgt taatttcgtt acttattgcg ttacggacta cgcttaaaaa aaccttcttc
    12061 gcgaggcgat accatgagcc gattcatccc catagaatta caccatgcca gccggctgtt
    12121 aaatcatggc ccaactgtca tgatcaccag tttcgatgag caatcccagc ggcgtaacat
    12181 tatggctgca gcctgatcaa tgccggtgga gtttgaacct ccacgcgtga cgattgtggt
    12241 agataaatcg acatggacca gggagttgat tgaacgtaac ggtaaatttg gcatcgttat
    12301 cccgggcgtt gcagcaacta actggacgtg ggcagtggga agtgtgtcgg gtcgtgaaga
    12361 agataaattt aattgctatg gcattccggt tgtgagaggc ccggtatttg gtttgcctct
    12421 ggtcgaggaa aaatgtctgg cgtggatgga gtgtcgattg ctacctgcga cttctgcgca
    12481 agaagaatac gacacgctgt ttggcgaagt agtatcagca gcggcagacg cacgggtatt
    12541 tgtcgaaggc cgctggcagt ttgatgatga taagctcaat acgttgcatc atttaggtgc
    12601 tgggacgttt gttaccagcg gcaagcgtgt tacggcgggt taagcattac atgctgtaac
    12661 cgggtttctt tatcaaagcc tccatttggg gcgcgatttc ggcatcccag atagcttgcc
    12721 aagattctgg ttgaatctgt tgtagagcaa tgcttatcga actgtcttta ctgttcagat
    12781 ggcgaataat aacgtcggta atatctgcag caagtgctgc tttttgttgt tcgtccagtt
    12841 cacgcggaaa acatttaatg tctatgtgcg gcatgattac gctccttttg aaagatgtca
    12901 gtaaaattaa cacacattgt gaatgtcgtg tgtccggtaa agcgttttta cgacaataa
//
LOCUS       DAZFBR010000123        12942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFBR010000123 DAZFBR010000000
VERSION     DAZFBR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.123"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..202
                     /locus_tag="ACHJZ2_003999"
     CDS             <1..202
                     /locus_tag="ACHJZ2_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO4570023.1"
                     /translation="RREFAIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLR
                     DQIALRVTEAGADDEEELQQEE"
     gene            202..2304
                     /locus_tag="ACHJZ2_004000"
     CDS             202..2304
                     /locus_tag="ACHJZ2_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4570024.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSSSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPGWASRETWLESVPDSTEPSPSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            2301..2513
                     /locus_tag="ACHJZ2_004001"
     CDS             2301..2513
                     /locus_tag="ACHJZ2_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570025.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            2441..3565
                     /locus_tag="ACHJZ2_004002"
     CDS             2441..3565
                     /locus_tag="ACHJZ2_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4570026.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLNFFQYSAQL"
     gene            3687..4022
                     /locus_tag="ACHJZ2_004003"
     CDS             3687..4022
                     /locus_tag="ACHJZ2_004003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4570027.1"
                     /translation="MASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIR
                     GGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEP
                     QHTDDQSEE"
     gene            3967..5994
                     /locus_tag="ACHJZ2_004004"
     CDS             3967..5994
                     /locus_tag="ACHJZ2_004004"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO4570028.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLNINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGKPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPVYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            6081..6404
                     /locus_tag="ACHJZ2_004005"
     CDS             6081..6404
                     /locus_tag="ACHJZ2_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO4570029.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            6397..6672
                     /locus_tag="ACHJZ2_004006"
     CDS             6397..6672
                     /locus_tag="ACHJZ2_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO4570030.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            6684..7262
                     /locus_tag="ACHJZ2_004007"
     CDS             6684..7262
                     /locus_tag="ACHJZ2_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4570031.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAGSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLAQR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            7259..7660
                     /locus_tag="ACHJZ2_004008"
     CDS             7259..7660
                     /locus_tag="ACHJZ2_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4570032.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVSALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            7671..8414
                     /locus_tag="ACHJZ2_004009"
     CDS             7671..8414
                     /locus_tag="ACHJZ2_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4570033.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            8475..8861
                     /locus_tag="ACHJZ2_004010"
     CDS             8475..8861
                     /locus_tag="ACHJZ2_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4570034.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            8870..9199
                     /locus_tag="ACHJZ2_004011"
     CDS             8870..9199
                     /locus_tag="ACHJZ2_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4570035.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            9171..12236
                     /locus_tag="ACHJZ2_004012"
     CDS             9171..12236
                     /locus_tag="ACHJZ2_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4570036.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMPGAVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEARKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12236..12565
                     /locus_tag="ACHJZ2_004013"
     CDS             12236..12565
                     /locus_tag="ACHJZ2_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4570037.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            12575..>12942
                     /locus_tag="ACHJZ2_004014"
     CDS             12575..>12942
                     /locus_tag="ACHJZ2_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4570038.1"
                     /translation="MQDIPQETHHETTRLTQSAQAVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSSARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
BASE COUNT         3144 a         2999 c         3964 g         2835 t
ORIGIN      
        1 cagacgggag tttgccatct gggcaaaagc ggtcgtgcag gtgctggaga cattaccgga
       61 tattctggaa cgtgactgtg gtctgcagcc tgccgctgtg agccgtgttc agtccattat
      121 tgatgatctg cgcgatcaga tagccctgcg ggtgaccgaa gcaggtgcgg atgatgagga
      181 ggaattacag caggaggagt aatgctgaat caggaaaccg caaaggcagc acgaaccgat
      241 tcaggttata tccttcgcgc accgagacga atgcgggttg ctgatgccgt tgctcagtat
      301 atgcgggtgc ccatgggggc cgggaactca gtcccgtggg atccgctggt ggcaccgtat
      361 gttattgagc cgatgaactg cctggcctcg cgtgaatacg acgcagtgat atttgttggc
      421 ccggcacgaa ccggcaagac tatcggcctg attgacggct gggtgattta caacgtgatt
      481 tgcgatcctg cggatatgct gatcattcag atgacggagg aaaaagcccg cgaacactcc
      541 aaaaaacgac tcgccagaac gtttcgcgtc agcccggaag tggtcagtcg cctgagtccg
      601 aacaaaaatg acaacaacgt ttatgacaga acattccttg ctggcaacta cctgaaaatc
      661 ggctggccgt cagtcaatat catgtcctca tcagattata aatgcgtggc gctgacggat
      721 tatgaccgtt ttccggaaga tattgatggc gagggggatg ccttctctct tgcctcaaaa
      781 cgtaccacca catttatgtc cagtggtatg acgctggtgg agagttcccc cggcagggat
      841 gtgaaggatg tgaaatggcg acggacttca ccgcatgagg ctccaccaac cacggggata
      901 ctgtcgctct ataaccgtgg cgatcgccgt cgctggtact ggccctgtcc acactgtggt
      961 gagtattttc agccctgcgg cgatgtggtt gctggtttcc gtgatattgc cgatcccgtg
     1021 ctggcaagtg aggcggctta tattcagtgt ccttcctgtt caggacggat tatgcctgaa
     1081 caaaaacgtg agctgaacgg acgtggggtc tggttgcggg atggtgaatc catcaatgcg
     1141 gatggtagtc gttatggtga tccccgacgc tcacgtattg cgtcattctg gatggagggt
     1201 ccggcagctg cttaccagac actctcgcaa ctcgtttaca aactgcttac tgcagaacag
     1261 gaatacgaga caaccggaag tgaagaaaca ctcaagacgg ttatcaatac cgactgggga
     1321 ttaccttatc ttccccgcgc cagcatggag caacgaaaaa gtgaactgct tgagcagcgg
     1381 gcagagccag ttccttccag cagtgtgccg gatggcgtta atttccttgt ggcgacagtg
     1441 gatgtgcagg cgggacgtca tcgccgtttt gtggttcagg taacgggcta tggcagccgt
     1501 ggcgaacgct ggattattga tcgttacaac atcacgcagt cattgcgcgg tgacagcgac
     1561 ggtgagagcc agcgaattga tccggccagc tatccggaag actgggatgt cctgctgacg
     1621 gatgtttttc ataaaagctg gccgctggcc tccgatcctt ctcaacaaat gcgactgatg
     1681 gcaatggcgg tggactccgg cggtgaagac ggggtcactg ataatgccta taaattctgg
     1741 cgtcgttgcc gtcgtgatgg ccttggtaaa cgtatttacc tgtttaaggg cgacagcatc
     1801 cggcgagcaa aactgatcac ccgtacattc cctgataaca ccggacgaac gggccgacgg
     1861 gcgcaggccg caggtgatgt gccgctctgg cttcttcaga cggatgcact gaaagaccgg
     1921 gtgaataacg cgttatggcg tgactctcca ggtcccggct atgtgcattt ccctgactgg
     1981 ctggggagct ggttttacga cgaactgacg tatgaagagc ggagtagtga cgggaaatgg
     2041 agtaagccgg gtcgcggtgc caacgaagct tttgacctga tggtgtatgc cgaggctctg
     2101 gtcattctgc atggatacga aaagatccgc tggccggatg caccggggtg ggcgagccgg
     2161 gaaacctggc tggagagtgt cccggacagt accgaaccgt caccctcacc ggaaccggta
     2221 tccacgcctg ttaaaaaaca aaaacggaag aaaacagtaa ctgacgatgt taacccctgg
     2281 ctgacttccg gaggatggtt atgaaccaga atgatatcga agccatgatt cagcgttata
     2341 cggaagctga aatggcggtg ctggacggaa aatccgtcac ctttaatggt cagcagatga
     2401 ccatggaaaa cttatctgag atccggcagg gacggcagga gtgggagcgc cgccttgcgg
     2461 ctctgattac acgacgacgg gggcatcccg ggtaccggct ggcgaggttc tgatggcaat
     2521 tcttgatgat gtgattggcg ttttttcacc aggatggaaa gcggcaaggc tgcgttcccg
     2581 tgcggtgatc caggcttatg aggccgtaaa aacgacgcgg acacacaaag cccggcggga
     2641 aaaccgaact gccgaccagt taagccagta cggggccgtg tcgttacgtg agcaggcccg
     2701 ttaccttgat aacaaccacg atctggttat tggtgtattt gacaagctgg aagaacgggt
     2761 ggtggggaaa aacgggatta ttgtcgagcc acatccggta ttacgcaatg gggccattgc
     2821 ccgtgatctg gcagcggaga tacgcacccg atggagtgaa tggtctgtca gtccggaagt
     2881 caccgggcag tttacccgtc cgatgctgga acgtctgatg ctgcgtacct ggctgcgcga
     2941 tggtgaggtg tttgcccaga tggtttccgg gcgcataaac agcctgacgc cttctgccgg
     3001 tgttcatttc tggctggagg cgctcgagcc agactttatt cccatgacca gtgatgagag
     3061 caacaggctg aatcagggcg tgtttgttga tgactggggg cgtcccgaaa aatatctggt
     3121 gtataaaagc cgtcccgtat ccggacggca gatggaaacc aaagaagtgg atgcagagcg
     3181 aatgctgcat cttaaatttg ttcgccgtct gcaccagatg cgcgggacgt ctttgttgtc
     3241 cggtgtgctg atccgcctca gtgccctgaa agagtatgag gattctgagc tgactgcagc
     3301 aaggatcgcc gctgctctgg ggatgtacat ccggaaaggc gatgggcaga gctatgaacc
     3361 ggatggtaat ggcagcaagg ataaggaacg cgagcttacc attcagccag gcattattta
     3421 cgacgatctg aaacccggcg aagaaatcgg aatggtgaag tcggatcgcc ccaatcctaa
     3481 ccttgaaact tttcgtaatg gtcagttgcg tgccgtggcg gcgggcagtc gtctgaattt
     3541 tttccagtac agcgcgcaac tataacggca cttacagcgc ccagcgtcag gagctggttg
     3601 aatccactga tggctacctg atcctgcagg actggtttat tggtgccgtc acccgcccga
     3661 tgtatcgtgc ctggctgaaa caggctgtgg catccggtgt tatcaggcta ccccgcgatc
     3721 ttgaccgttc ttcactgtat accgcggtgt attccggacc ggtgatgccg tggattgacc
     3781 ctgttaagga ggctgaggcc tggaaaattc agattcgtgg tggagcagcg acagaatcag
     3841 actgggtacg tgcaggtggt cgtaatccgg atgatgtcaa acgtcggcgc aaggccgaaa
     3901 ttgatgaaaa ccgcaagctg gatctggtat ttgataccga tccggccagt gataaaggag
     3961 gcagcagtgc cgcaacgaaa cgacaggagc cgcagcacac cgacgaccag tccgaagaat
     4021 aattcctggt tcaggatgca ggctggtcac cagagtgacg cggatattta tatttatgac
     4081 gagattggtt tctggggtgt tacagcgaag cagtttatca gtgatctgaa tgcactgggc
     4141 gatatcaccc acattaatct caatatcaat tcaccgggtg gcgatgtctt tgaaggcatc
     4201 gccattttta atgcactgaa aacacatggt gcgtccatta ccgtttatgt cgacggtgtg
     4261 gcggcgtcaa tggcgtcggt cattgcgatg gtgggaaaac cggtcattat gccggaaaac
     4321 tccttcatga tgattcataa accatttggc tttacgggcg gtgatgcgga ggacatgcgc
     4381 acctatgccg acctgctcga taaagttgag gcggttctgt tacccgttta tgcacagaaa
     4441 accgggaaaa ccaccgatga aattgctgcc atgctggcgg atgagacctg gatgtccggt
     4501 gccgaatgtc tggcacatgg atttgctgat caggtgacgc cagccgttaa ggcaatggca
     4561 tgtattcagt caaaacgtac agaggaattt aaaaagatgc cggaatccat tcgaaacatg
     4621 attactccgc cacgcaacag tgctccacgc gtacaggatg atggacctgc agcctcccgg
     4681 acgccagtgc aggcagcagc acctgtggtg gatgaaaaca gtatccgtgc gcaggtactg
     4741 gcagagcaaa aagcgcgtgt aaacggtatt aatgatctgt ttgccatgtt tggcgggcgt
     4801 tatcagacgc tgcaggctca gtgtcttgcc gatcctgaat gttcgctgga gcaggcccgc
     4861 gaaaagctgt tgaacgagat ggggcgcgag tccacgccat ctaataaaaa taccccggct
     4921 catatttatg ccggaaacgg taattttgtg ggggatggga tccgccaggc gctgatggcg
     4981 cgtgccggat ttgaaaaaac cgaacgcgat aatgtctaca acgggatgac cctgcgtgaa
     5041 tatgcccgta tgtcactgac tgaacggggt attggggttt ccagttataa cccgatgcag
     5101 atggtcggtg cggcgttcac acacagtacg tctgacttcg gtaatattct gctggatgtt
     5161 gcgaacaaag ccattctgca gggctgggaa gatgcccctg aaacctatga acagtggacg
     5221 cggaaaggtc agttgtctga ttttaaaatt gcccatcgtg tgggtatggg gggcttcagt
     5281 gctctgcgtc aggtgcgtga aggggcggaa tataaatacg tcaccaccgg agataaacag
     5341 gccactattg cactggcgac ctatggcgag ctgttcagta tcacccgtca ggccattatc
     5401 aatgatgatc tgaatatgct gaccgatgtc ccgatgaaac tgggccgtgc ggcgaaatcc
     5461 actattgccg atctggttta tgccattctg acgtctaacc cgaaaatctc cacagataat
     5521 gtaagtctgt tcgataaagc gaaacatgca aacgtactgg agagcgctgc aatggacgtg
     5581 gcatcgctgg ataaagcccg ccagttgatg cgcgttcaga aagaggggga gcgtcatctg
     5641 aatattcgtc ctgcgttcgt actggtaccg acggcgatgg agtctgttgc taaccaggtc
     5701 attcgctcct caagtgtcaa gggggctgac attaacgccg gtattattaa cccggtgaaa
     5761 gattttgcga ccgttattgc agagcctcgt cttgatgata acagccagac caccttctac
     5821 ctggctgcgt caaaaggctc cgatacgatt gaagtggctt atctcaacgg tgtggatacg
     5881 ccatatattg atcagatgga gggcttcagt gtggatggcg tgacaacgaa agtgcgtatt
     5941 gacgccggtg tcgcgccagt tgatcaccgc ggtctggtga aatgtacggc gtaaacgtcg
     6001 cagacaacaa ctctgatggc ccgtaagggc tttttttgta cctgaaatca gcccctgaac
     6061 ggggctgtgc ggagacagtt atggcaaaga attttgtaga agaaggaaaa acggtggcga
     6121 ttgttgccag tgcagccatc agcagcggag atctggtgca ggtgggcgat gtttttgcgg
     6181 tggcgctgac cgatattcca cagggtgaaa caggcgacgg catgaccgaa ggtgtgttta
     6241 tgctgcctaa gctgaaaacg gatgacatga aaacgggtaa gaaggtttat ctgaagtcag
     6301 gaaaagttca gctgactaac agcggctctg atccgctggt cggggttgtc tgggcagatg
     6361 ccggaaccag tgcagaagaa gtgccggtaa aactcaatgt ctgatccctt ttcccggctg
     6421 gcagcgcgta tggatgctat cacggtcaga aagatgggaa agacagcctc gattaatgat
     6481 gtcgatatga ctgtgatccc gggagaaaca ctggcagagc tgaatgctct gtccggacct
     6541 gcggtctctc tggtggtgtt ttcttcggga taccgcccac ggcgcgggga tcgcgttgtt
     6601 tatgacggac aacaatggac ggtcacacgg catgaacgct ttaacggtaa gccaatgatc
     6661 tttattgagt aaagaggtgt gggatgaagg ggcttgagaa tgccatccgc aatctgaaca
     6721 gccttgatac ccgtatggtg ccacaggcca gcgcatgggc gataaaccgt gtggcacaga
     6781 aagcgggctc ggttgccacc cggcaggttg ccgggaatac cgttgcggga gataaccagg
     6841 tgaaagggat ccccctgaaa ctggttcgtc agcgtgtccg ggtgtttaaa gccagtccgt
     6901 caggaaaaat gacggccagg atccgcgtta accggggcaa tctgcccgcc attaagctgg
     6961 ggacagcccg ggtcagactg gcccagcgtg gtggaaaact gcagtaccgt ggcagtgtgc
     7021 tgaaggtggg taaatatctt ttccgggatg cgtttattca gcaactggcg aatggtcgct
     7081 ggcatgtgat gcggcgtatt gatggcaaaa atcgttaccc cattgatgtg gtgaaaatcc
     7141 cgctgtccgg accgctgaca caggcatttg aagatgcccg cgaccgcatc attgctgcgg
     7201 aaatgccgaa acagctgggg tatgcactga aacaacaact gaggttatgg ctgacccgat
     7261 gaaccgacat acacaaatcc gccaggtcgt actggcacgc cttcgggaac agtgtggaga
     7321 cagcgccacg ttttttgacg ggcttccggc atttgttgat gcgcaggaac tgcctgccgt
     7381 ggcggtgtgg ctgagtgatg ctcagtacac cggaaaaatg acggatgaag atgactggca
     7441 ggctgttctg catattgctg tcttcatccg ggcacaggca ccggattcag agctggatat
     7501 gtggatggag agcaccattt tcccggccct gaatgatgta tcggcacttt ccggactcat
     7561 cgacaccctg atcccactcg gttttaacta tcaacgtgat aatgagatgg ccacctgggc
     7621 gatggcggaa atcacgtacc agatcacgta cacgaattaa ggaggtggta atgaccacgc
     7681 caaatccact ggcaaagacg aaaggtgcgg ggacgacgtt ctggatgtat accggcaacg
     7741 gcgatgcgtt tgcgaaccct ttgtcggaca ctgactggct gcgtcttgcg atggtgaagg
     7801 atctgcaacc tggcgaaatg accgctgatg cagaagatga cacttatctc gatgatgaag
     7861 atgcagactg gaaaacgaca acccaggggc agaaatccgt tggtgatact tcggcgacgc
     7921 tggcctggcg tccgggtgac agcgggcaga aaaaactggt tcagttgttc gactccggtg
     7981 aagtctgcgc gtttcgtatc aaatatccca acggcactgt tgatgttttc cgcggctggc
     8041 tgagctcact gggtaaaacc attgcctcaa aagacgtgat gacccgcaca gtgaaaatca
     8101 gcggtgtggg gcgtccatat ctggcagagg agggcactga aactgtgggc gttaccgggc
     8161 tgacggtggc accggcatcc gccagtgtca atgtgggagc aaccaccacg ctgaccttta
     8221 cagtaaaacc tgacggagcc agtgacaaag cgatcagtgt gcattcgaca gatccacaga
     8281 cagccactgt gaccctgaac ggacttgtgg ccacggtgaa aggcgtgaag cagggcagcg
     8341 tcagcattgt gggcatgacc gctgacggga attttgtggc tgtggctgcg gtgactgtca
     8401 gcgctgcagg ttaacaggac gatactcatc atttgccccg gttatccggg gcttttttgc
     8461 aggtggagaa catgatgttt ctgaaacagg gcacgtttaa ttatgaaaag cagtccgtgg
     8521 tgctcagtga gctgtccggg ctgcagagaa ttgaatatct ggcgtttgtt cagcagcgaa
     8581 cggcaaagtt tgatgccgaa gagggagaac tgccggaggc tgaacgacag attgcttttc
     8641 tgcggatggg gatggatatc aatgcctggc tggtttcccg ctcactgtgg aatgcggaac
     8701 agtctcagga tgttgagacg ctttgcgcat ccgttattac aacatggtcg tatgatgccc
     8761 tgggagcggg ggcggagatg gttctgtcgc tgagcggtat gggagccatt gagaatgccg
     8821 gggatttgga gcatgaggtg ctgacgccgg aaaagtcctg acgcgggaaa tgcagtttgt
     8881 catgcggctt gcccgggagt tccggcgggc agactggcgg cggatgctgt cggaaatgtc
     8941 ggccactgag cttggtgagt ggggcgatta tttccggatg cagagcttca gtgatgtgtg
     9001 gatggatgcg cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggtagcag
     9061 tgatgctgcg gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac
     9121 ggacgaagaa ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga
     9181 tagccaacct ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc
     9241 gtatcaaaaa ccttctgaat ggtgcagcca gcgatgcaga acggtcttct gcccgtatgc
     9301 agcgttttat ggagcgtcag actcaggccg cccggcagac aatgcaggcg gcttcttcgg
     9361 ctgcaacagc cgcatccgtc catgcgcaga cggtggagaa gagcgcacag gctcatgaac
     9421 gcatggcccg cgaggtggag caaacccgcc agcgtatgga ggcactgagc cagaaaatgc
     9481 gcgaggaaca ggcgcaggcc atggctctgg cggaggctca ggataaagcg gctgctgcgt
     9541 tttatcgtca gattgacagt gtgaaacagg ccggtgcggg gctgcaggaa ttacagcgta
     9601 ttcagcaaca gatccgacag gccagaaaca gtggcgggat tggtcagcag gattatctgg
     9661 cgctgatttc tgaggttacg gcgaaaaccc gtgttcttac acaggctgag gaagaggcta
     9721 cccgacagaa agtggcgttt atccgtcagc ttaaagagca ggcaacccgc cagaatcttt
     9781 cttcttctga gttgcttcgt gctaaggctg cccagctggg ggtaagcagt gctgcagaag
     9841 tgtatatccg caaaatggag caggcaggaa aagccacgca ttcgctgggt ctgaaaagtg
     9901 cagcagcccg tcaggagata ggcgttctga taggtgaact ggcccgcggc aatttaggtg
     9961 cgctgagggg atccggaata acgctggcta accgtgccgg gtggatagac acactgatgt
    10021 caccgaaagg catgatgcca ggagcggtta ttggcggtat tgccgcggct gtctatggtc
    10081 tgggtaaagc ctggtatgac ggtcagaagg agggggaaga atttaaccgc cagctgtcgc
    10141 tgacggggca ttatgccgga gtcactgccg ggcagctgtg gacgctcagt cgtgctattt
    10201 ccgggaatgg tatcacgcaa catgctgcag ccggtgcgct ggctcaggtg gtggggagtg
    10261 gtgcatttcg tggaaacgat atcggtatgg tggcgagagc tgccgcacag atggagcgat
    10321 cggttggcca gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg
    10381 taaatgccgc gaaggctctg gacaatgagc tgcattttct tactgccact cagcttgagc
    10441 agatacgcgt ccttggggag caggggcggt ccagtgatgc tgcacggata gccatgtctg
    10501 cactggcaga ggaaaccggt cggcgtactg cggatattga taataacctc aatgcgcttg
    10561 gcagtacgct gaagtatctg tctgatttgt ggagtcgttt ctgggatgcg gccatgaata
    10621 ttggtcgtga agactcgctg gatgaacaga ttgccgcttt acaggagaaa gtgtcgcggg
    10681 cgaaaagact cccctggacg gcatcatctt ctcaggttga atacgatcag cagcgtctta
    10741 acgatcttca ggagaaaaaa cgccagaagg atttgcagga tgcaaaagag caggcagagc
    10801 ggaattatca ggagcaacag aaacgccgta atgctgaaaa tgctgcactg aaccggatga
    10861 atgaaacgga agcagcacga catcagcgtg aaatagcgcg tattaatgcc atgcagtacg
    10921 ccgatcaggc tgtcagggat gcggcgatac aacgtgaaaa tgaacgttac gagaaagccc
    10981 tggcatccgg taagaaaaaa acacgcgaaa cccgtaatga tgaggccacc cggttattgc
    11041 tgcagtacag tcagcaacag gcacaggtgg aaggacagat tgctgctgcc agacagtcag
    11101 caggcattgc cacggaaagg atgacagaag cgcgtaaaca gcttctggct ctgcagcagc
    11161 gcatcagcga cctggacggg aaaaaactga cggcagatga aaagagtgtg ctggcccgta
    11221 aagatgaact gattcaggca ctgacgctgc tggatgtaaa acagcaggag cttcagaaac
    11281 agacggcact caacgagctg aagaaaaaaa caattcagct gaccagtcaa ctggctgaag
    11341 aagagcgcgc tcagcgtcag caacatgacc tggatatcgc cacggtgggt atgggtgatc
    11401 agcagcgaca gcgatatcag gtacaactga gtcttcgcca gaaataccag caacagctgg
    11461 agcagttgag gcgggatagt gagcagaaag ggacatataa cacggatgac tacagaaagg
    11521 ccgagcaggc gctgacggag agcttgaacc gacaactgaa tgagaatcgc cgttactggc
    11581 aacagcttga agttgtacag ggtaactgga aaaacggagt cctgcgtgca tttcaggatt
    11641 ttaccgtgga tgcagataat acggcagaaa cagcagaaca ggtgttctcg tcagccttca
    11701 gcaacatggg aaatggcctg gcaacttttg tcactaccgg caaactcaat ttcaaatcct
    11761 tcacctcttc tgtgctgtca gatatggcga aaatcctggc gcaggcaacc atgatgaaat
    11821 cgataaaagg gattggcagt gtactgggat ttgatctcag cagcctttcc ctgaatgcca
    11881 atggggggat ttatcagtct gctgatttga gtcgttacag tggcacggtg gttaaccgtc
    11941 cgacgttttt tgcttttgca aaaggcgcgg gtgtgatggg ggaagcggga cctgaagcca
    12001 ttctgccatt gcgtcgtggt gctgacggta agctgggggt tgtggcggat attgggggtt
    12061 caggtatggc gatgttttcc ccgcagtaca acatcgagat caataacgat ggcacgaacg
    12121 ggcagatagg tccggctgcc ctgaaggcgg tttatgacct cgggaaaaaa gcggcagcgg
    12181 actttatgca acagcaggcc cgtgatggtg gtcggttaag tggagcatat cggtaatgga
    12241 gacgtttcac tggaaagtgc gcccggatat gaatgtggta tcagagccga aagtggtgac
    12301 agtgaagctg ggcgatggtt atgaacagcg tcgtgcggcg ggactgaata accagttgtc
    12361 gacttacagc gtgacgatac gtgttcgtaa atgtgaacac ccatctttaa aagcctttct
    12421 ggaacggcac ggtggcgtcc gtgcatttca gtggacgcca ccttatgact ggaaaccgat
    12481 cagggtggtt tgtcgtaaat ggtcggcaag cgtgggggcg ctgtgggtaa ccataacggc
    12541 agattttgaa caggtcgtgg cataggaggc tctgatgcag gatattccac aggaaacaca
    12601 tcatgagacg acacgcctca ctcagtcagc ccaggcggtg ctctgggaaa tcgatctgac
    12661 agaggtcggt ggtgaacgtt attttttctg taatgagcag aacgaaaaag gtgagccggt
    12721 tacctggcag gggcggcagt atcaggcata ccccattcag gggacgggat ttgaactgaa
    12781 cggcaagggc agttctgccc gtccgacact gacggtttct aacctgcacg gcatggtcac
    12841 ggggatggcg gaagacctgc agagtctggt cggcggaacg gtggtccggc gtaaggttta
    12901 cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac ag
//
LOCUS       DAZFBR010000124        12422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFBR010000124 DAZFBR010000000
VERSION     DAZFBR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.124"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1432
                     /gene="ygcU"
                     /locus_tag="ACHJZ2_004015"
     CDS             <1..1432
                     /gene="ygcU"
                     /locus_tag="ACHJZ2_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO4570039.1"
                     /translation="VDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHGIYTLPIPA
                     AVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDGSAMNQIIN
                     IDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRSIGQFSTLY
                     GAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITEVTVKIFKF
                     TPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLI
                     FMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILK
                     TGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFV
                     YDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAW
                     ALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            1526..2862
                     /gene="ygcS"
                     /locus_tag="ACHJZ2_004016"
                     /pseudo
     CDS             1526..2862
                     /gene="ygcS"
                     /locus_tag="ACHJZ2_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2840..3619
                     /gene="ygcR"
                     /locus_tag="ACHJZ2_004017"
     CDS             2840..3619
                     /gene="ygcR"
                     /locus_tag="ACHJZ2_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO4570040.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3616..4476
                     /gene="ygcQ"
                     /locus_tag="ACHJZ2_004018"
                     /pseudo
     CDS             3616..4476
                     /gene="ygcQ"
                     /locus_tag="ACHJZ2_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(4624..5199)
                     /gene="ygcP"
                     /locus_tag="ACHJZ2_004019"
     CDS             complement(4624..5199)
                     /gene="ygcP"
                     /locus_tag="ACHJZ2_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO4570041.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(5216..5476)
                     /gene="ygcO"
                     /locus_tag="ACHJZ2_004020"
     CDS             complement(5216..5476)
                     /gene="ygcO"
                     /locus_tag="ACHJZ2_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO4570042.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(5467..6738)
                     /gene="ygcN"
                     /locus_tag="ACHJZ2_004021"
     CDS             complement(5467..6738)
                     /gene="ygcN"
                     /locus_tag="ACHJZ2_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4570043.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPVLLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWNLAGDVIRSVRCL"
     gene            complement(6816..7178)
                     /gene="queD"
                     /locus_tag="ACHJZ2_004022"
     CDS             complement(6816..7178)
                     /gene="queD"
                     /locus_tag="ACHJZ2_004022"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO4570044.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            7497..9296
                     /gene="cysJ"
                     /locus_tag="ACHJZ2_004023"
     CDS             7497..9296
                     /gene="cysJ"
                     /locus_tag="ACHJZ2_004023"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO4570045.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            9296..11008
                     /gene="cysI"
                     /locus_tag="ACHJZ2_004024"
     CDS             9296..11008
                     /gene="cysI"
                     /locus_tag="ACHJZ2_004024"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO4570046.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            11082..11816
                     /gene="cysH"
                     /locus_tag="ACHJZ2_004025"
     CDS             11082..11816
                     /gene="cysH"
                     /locus_tag="ACHJZ2_004025"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4570047.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            12080..12232
                     /locus_tag="ACHJZ2_004026"
     CDS             12080..12232
                     /locus_tag="ACHJZ2_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4570048.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2996 a         3155 c         3415 g         2856 t
ORIGIN      
        1 tgtcgaccag ctaaaggaaa ttgttggtgc agatcgcgta attaccgatg aaacagtatt
       61 aaagaagaac agtattgacc gttttcgtaa atttccggat attcatggca tttatacttt
      121 gccgattccg gcagcggtcg taaaactcgg ttccacagag caagtatccc gtgtgctgaa
      181 ttttatgaat gcgcacaaaa ttaacggtgt gccgcgtacc ggtgcttccg ccaccgaagg
      241 tgggctggaa actgttgtag aaaactcggt ggtgctcgac ggttccgcca tgaatcaaat
      301 cattaatatt gatattgaga atatgcaggc gacggcgcaa tgtggtgttc cgctggaggt
      361 gctggaaaac gcgttgcgtg aaaaaggtta caccacgggg cattctccgc agtcaaagcc
      421 gctggcgcag atgggcggcc tggtagcaac ccgcagtatc gggcagttct ccacactcta
      481 cggcgcaatc gaagatatgg tcgttggtct ggaggcagtg ttggcagatg gcaccgtcac
      541 acgcattaaa aacgtgccac gccgcgcggc tggcccggac attcgtcaca tcatcatcgg
      601 caacgaaggt gcattgtgct atatcactga agtaacagtg aaaatcttta aattcacccc
      661 ggaaaacaac ctcttctacg gctatatcct ggaagacatg aaaaccggct tcaacatcct
      721 gcgtgaaatc atggtggaag ggtatcgtcc gtcgatcgct cgtttgtatg acgctgaaga
      781 tggcacccaa cacttcaccc attttgccga cggaaaatgc gtgctgatct ttatggctga
      841 aggtaaccct cgcattgcga aggcgacggg cgaagggatt gcggaaatcg ttgcccgcta
      901 cccgcaatgc caacgcgtgg acagcaagct gatcgaaacc tggtttaaca acctgaactg
      961 gggaccggat aaagtggctg ccgaacgtgt gcagatcctc aaaaccggca acatgggctt
     1021 tactaccgaa gtgtccggct gctggagctg catccacgaa atctacgaaa gcgttattaa
     1081 ccgtattcgt accgagttcc cgcacgccga cgacatcacc atgctgggcg gtcactcctc
     1141 tcatagctat cagaacggca ccaacatgta cttcgtctac gactacaacg ttgtcaactg
     1201 taagccggaa gaggaaatcg acaagtacca caatccgctc aacaaaatca tttgcgaaga
     1261 aaccattcgc ctcggtggtt cgatggtgca ccaccacggt atcggtaaac atcgcgttca
     1321 ctggagcaag ctggaacacg gcagcgcgtg ggcgttgctg gaagggctga aaaagcagtt
     1381 cgatcctaat ggcattatga atacgggtac tatctatccg attgaaaaat aatgtgtcag
     1441 gcagcgttcc gcgatgatgg ggcgttgcct tttcggcttc tcaggcgaga agccgttctt
     1501 attaccggac aatgaagggg taaagatgaa cacttcaccg gtgcgaatgg atgatttacc
     1561 gcttaaccgt tttcactgtc gaattgctgc gctcactttc ggcgcacacc tgaccgacgg
     1621 ttatgttctc ggcgtcattg gttacgccat tattcaactt acgcccgcca tgcaactgac
     1681 gccgtttatg gcgggaatga tcggcggctc ggcgcacctt ggtttgtttc ttggcagcct
     1741 ggttcttggg tggatctccg accatattgg tcggcaaaaa atcttcacct tcagcttttt
     1801 gctgattacg ctcgcttcgt tcttgcaatt ttttgccacc acgccagagc atcttattgg
     1861 actgcgcatt ttgatcggca ttggtctggg aggcgattac tcagtaggtc acaccttgct
     1921 ggctgaattt tccccgcgcc gccatcgcgg tgttttgctg ggcgcattca gcgtggtgtg
     1981 gaccgtaggc tatgtgttgg caagtattgc cggacatcac tttatttccg aaaacccgga
     2041 ggcctggcgc tggctgctgg catcggcagc tctgcccgcg ttgttgatta cgttattacg
     2101 ctggggaacg ccagaatcgc cacgctggct actgcgccag gggcgttttg cagaagctca
     2161 cgctatcgtg catcgctatt ttggtcccca tgttttactg ggcgatgaag tggcaacggc
     2221 gacccataaa cacatcaaaa ccttgttctc ttcgcgctac tggcggcgca cggcgtttaa
     2281 cagcgtcttc tttgtctgcc tcgtaatccc atggtttgtg atttatacct ggctgccaac
     2341 tatcgcccag actattggac tggaagatgc gctgactgcc agcctgatgc ttaatgcgtt
     2401 gttaattgtg ggcgcgctgc tgggattagt tctgacgcac ctgctggcac atcgcaaatt
     2461 tttgctggga agttttttgc tgctggcggc aacgctggta gtgatggcct gtttgccttc
     2521 cggcagttca ttaacgctgc tgcttttgtt ctcttcagca ccaccatttc ggcagtcagt
     2581 aatctggtgg gcattttgcc tgcggaaagt tttcctactg acattcgctc gctgggcgtc
     2641 ggttttgcca ccgccatgag tcggttgggg gcggcggtaa gtactggcct gctgccgtgg
     2701 gtgctggcgc agtggggaat gcaagtcacc ttattgctcc tggcggcagt gttgttggct
     2761 ggttttgttg taacctggct atgggcacca gaaaccaaag cactcccgct ggtggcggcg
     2821 ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt aaagccgaac cggatgccgg
     2881 aatgctggcg gaaaaagagt ggcaggcggc ggctcatggt aatagcggac cggatatttc
     2941 gctactgcga agtttactcg gtgctgatga acaggctgcc gccgcgctgc tgttggcgca
     3001 gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc atgggggatg aacgggcgtt
     3061 gcactggctg cgctatctca tggctcttgg gtttgaggaa gctgtattgc tggagacggc
     3121 ggcagatctg cgctttgcgt cggaatttgt cgctcgccat attgccgaat ggcagcgtca
     3181 gaatccgctg gatctgataa ttaccggctg ccaaagcagc gaagggcaga atgggcaaac
     3241 gccatttttg ctggcggaaa tgctgggctg gccttgcttc acccaggtgg agcgtttcac
     3301 tctcgacgcg ccgtttatca ccctcgaaca gcgtactgaa catgggctgc gctgttgtcg
     3361 gattcgcttg cctgcggtga ttgccgtgcg tcagtgcggt gaagtggcct tgccagtacc
     3421 gggaatgcgc cagcgaatgg ctgcggggaa agcggaaatt atccgtaaaa ctgttgctgc
     3481 cgaattgcca gcgatgcagt gtctgcaact ggcaagagca gagcaacgac gcggagcgac
     3541 gctgattgat gggcaaacgg tagcagaaaa agcgcaaaag ctatggcagg actatttacg
     3601 ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa atcaggagaa tgccgcatta
     3661 gcgggctggc tggcggcgca ggatttcagc ggttgtaccc tggcgcactg gcaaatagaa
     3721 ccacagcccg tagtggtgga acaggtgctg gatgcgctgg tggaacagtg gcagcgaacg
     3781 cctgcgaacg ttgttctctt tccgccggga acgtttggcg atgaactgtc gactcggctg
     3841 gcctggcgtt tacatggtgc aagcatctgc caggtgactt cgttagagat tccaatcgta
     3901 agcgtgcgca aatcccactg gggaaatgcg ctaacggcga cgctgcaaac agaaaaacgc
     3961 ccgctatgtc tttccctggc gcgccaggcg ggagcagata aaaatgccac gttgccctct
     4021 ggtatgcagc aactgaacat agtcccaggt gcgcttccgg actggctggt tagcatagag
     4081 aacctgaaaa atgttacccg cgacccatta gctgaggcca gacgggtgtt ggtggtgggg
     4141 cagggcggag aggcagataa ccaggaaatc gctatgctgg cataaaagct gggagccgaa
     4201 gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg atgctgaaaa agtgattggt
     4261 atttccggac atctgctggc acctgaggtg tgtattgttg ttggcgcttc tggcgcagca
     4321 gcgttaatgg cgggcgtacg caatagcaag tttgtggtgg cgattaatca tgacgccagc
     4381 gcagcggtat tttcacaggc agatgttggc gtggttgatg actggaaagt gatgctggag
     4441 gcactggtga caaacattga cgcagaatgc caataacgtg ctgccggatg atcctgccta
     4501 cggttctgga ctggggacct gtaggcctga taagacgtgt taagcgtcgc atcaggcgct
     4561 gaatgtcgga tgtggcgtgc ggcctacaaa tcaaaccaaa ttcaattggt tacgattatc
     4621 ccgtcaaagc aattttttcg ctaacgtcca gactccggta ttcgtggtgg aaagcgccac
     4681 gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg cataccagcc cgccagcaat
     4741 cagcggttgg cggatttttt ccgtcaccca gcccagcact ttgggcatgc agcctggcag
     4801 gatctcaata caatccggat tcgattgcgc aacttgctta tcaatgttgt gaaacgaaat
     4861 cgaatcaaca ataaacaggc gatgaataca gaaaaaacct tccgctcttg ccgcttttag
     4921 catcgatgct ttggtgctga taattccatc cgcctcggtc accagtttca aaaactgaat
     4981 caccacttct ttatttgacg cgccttccag caaatcaaca tgaataaaag catatttccc
     5041 agcgttctta atctttttaa cgatttgact aatggtgcag atattgccgt acaagacgga
     5101 aataaactgg cattcagaat cgatggccag ttgcaggctg gcattatctt taacggcagc
     5161 aatcaccgga ttctggcgga gcaggtgtaa gaggggcata aattatcctt attatttatc
     5221 caaatcggta ggtgatgccg aatccggaag gcggatagcg ccactgttgt agtgtggatt
     5281 cgtcgcacag caaacggcag gtgccacact ccaggcagcc gtgataatca acgcgtaagt
     5341 caccttccgg tgtgagcgaa aaaagaccta ccggacaggc gttaatcaac cgttgtacca
     5401 tctgacgctc aacggagtca gccggaacaa tgtgcggcgc atcagcagcg cgccagagat
     5461 tacgggctac agacatcgaa cactcctgat aacatcgccc gccagattcc acaagccatg
     5521 acgacgtaaa tgacgccaga gtaactggcg cagcggtgga acaggtttat caccctgatc
     5581 ccataaatcg cgggaaatat cctgcattaa cgcaggccac gtacggtacc atcccgggcg
     5641 ttgcaaaagc accggaacat gctgataacg ctgtagaaca tcccacagca ggctgcgctc
     5701 tacgttgtga tgataaagcg gaaacagatt ttgcggctcg cggtgctggc aggcgcttat
     5761 cagtgtttgt gccgccgcct gcgcgccagt tagcgccgta tccatgccgc gcacagaaat
     5821 tccggtattg acgcaactgc gcaacgcatc gcccaccagc agccagccgt taccggcgta
     5881 ttgtaccggc atactgtgca agccaccttc tggcaccaga tgcgcaccat actccagtga
     5941 ttccgtgttt ttgataagcg ggcgcactgc cggatgcgtt ttaaagcgag caagcagctc
     6001 gcttgccgga acacgacttt gcgtaaggga agagagtggg caaacaatcc ctaacgagag
     6061 cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag tcatcacaga ttccgccgct
     6121 gaacagcaac gctgcgcctt cgttattctc cagatgaaaa cgttcttcaa tagcggatgt
     6181 ttccagcgac agcacttctt tgatccccaa cgccatcgct tcgccagcag gacgagtcac
     6241 taacccgtga cgttcagcca ggacgctgtt ggcaccttct gccagcacca cataacgggc
     6301 gcggagaata tcgtcaccac aaatgacacc acacaccctg ccgttttctt catacagtgc
     6361 atccaccgtc gcaccaggga tgcattcgac accttctttt tcggcttcgg caaccagcca
     6421 cggatcgaac cgtgcacgta atacactcca ggattcaccg ccgggctgta agctggaaaa
     6481 cgtcgttgcg caatccggcg ttaacaggga aaggctttcg tgagtgatgc gtcgttcaag
     6541 aggcgcggtc agatgaaatt gagggaggag ttccgcgagt gcatgggtat ataaccgccc
     6601 gccggaaaga tttttgctgc cggggatttc agcgcgttcc agtaacaaaa cggataaacc
     6661 cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata ccggcaccaa taataataat
     6721 gtcgcagtcg tcttccatgt catgccccag atatcctgaa cacaacaccc taacatagcg
     6781 ttacttaagg gaaattgacc gccgccacac tcttatcatt cgccgcgata gatacaacca
     6841 gcggtgcagg tttcttttac catcaccgca cttaacagcg gcacaacggg tttaacctga
     6901 tcccaaatcc attttgctaa aacttcgcta gttgggtttt ccagacctgg aatatcattg
     6961 agataatggt gatcgaggcg ctcataagtt ggcttaaacg ccgcttttag ttcagcgaaa
     7021 tcgataatcc agcccgtatg cggatcgact tccccggtaa tttccagtcg caccataaag
     7081 gaatgcccgt gcaggcgacc acatttatgc ccttccggga cgtgtggtaa gcggtgagcg
     7141 gcttcgaagg tgaaatcttt aaataacgtg gtggacatca taatttctct acaggacgat
     7201 aaaaccgccg tagagtaccg gaaaggctct tttttcgcat tatctaaaac gactttgttg
     7261 cgcaaaatcg ctgatttatc ttaatgattg gctaaattca tttgtttttc attaggttgg
     7321 ttaatctatt ttgttgttaa agactattgc taaaacaggt tagtcgattt ggttattagt
     7381 tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt aaccttacct tctcttctgt
     7441 tttatgggcg ctgacagggc gcagaaacag ctttgcttac tggaacataa cgacgcatga
     7501 cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc ggagcaactg gcacgccttc
     7561 aggcggccac gaccgattta actcccaccc agcttgcctg ggtttctggc tatttctggg
     7621 gcgtgctcaa tcagcagcct gctgcgcttg cagcgacgcc agcgccagcc gcagaaatgc
     7681 cgggtataac tattatctct gcttcgcaaa ccggcaatgc gcgccgggtt gctgaagcat
     7741 tacgcgatga tttattgacg gcaaaactga acgttaagct ggtgaacgcg ggcgactata
     7801 aattcaaaca aatcgccagc gaaaaactgc ttatcgtggt gacgtcaacg caaggagaag
     7861 gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct gttctccaaa aaagcgccga
     7921 agctggaaaa taccgctttt gccgtgttta gcctcggcga tagttcttat gaatttttct
     7981 gccagtccgg gaaagatttc gacagcaagc tggcggaact gggcggtgaa cgcctgctcg
     8041 accgtgtcga cgccgacgtt gaataccagg ctgctgccag cgagtggcgc gcccgcgtgg
     8101 ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc gcaatccgtc gctactggcg
     8161 tggtaaatga aatccacacc agcccgtaca gcaaagacgc gccgctggta gcgagccttt
     8221 cggttaacca gaaaattacc gggcgtaact ctgaaaaaga cgttcgccat atcgaaattg
     8281 acttaggtga ctcgggcctg cgttaccagc cgggtgacgc gctgggtgtc tggtatcaga
     8341 acgatccggc actggtgaaa gaacttgtcg aactgctgtg gctgaaaggc gatgaacctg
     8401 tcaccgtcga gggcaaaacg ttgcctctga acgaagcgct acagtggcac ttcgaactga
     8461 ccgtcaacac cgccaatatt gttgagaact atgccacgct tacccgcagc gaaacgctgt
     8521 tgccgctggt gggcgataaa gcgaagttac agcattacgc cgcgactacg ccgattgtcg
     8581 acatggtgcg cttctctccg gcgcaactgg acgccgaagc gctgattaat ctgctgcgcc
     8641 cgctgacgcc gcgtctgtat tccatcgcct cctcgcagga ggaagtcgag aacgaagtac
     8701 acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg cgcccgtgcc ggtggtgcct
     8761 ccagcttcct cgcggaccgc gtggaagaag agggcgaagt tcgcgtattt atcgaacata
     8821 acgataactt ccgcctgccc gctaacccgg aaaccccggt gattatgatt ggcccaggca
     8881 ccggcatcgc gccgttccgc gcctttatgc agcagcgcgc cgctgacgaa gcgccgggta
     8941 aaaactggct gttctttggc aacccgcact ttacggaaga tttcctctac caggtggagt
     9001 ggcagcgtta cgtcaaagag ggcgtgctga cgcgtatcga tcttgcctgg tcgcgcgatc
     9061 aaaaagaaaa agtttacgta caagacaaac tgcgcgaaca gggcgcggag ctgtggcgct
     9121 ggatcaatga cggtgcccac atttatgtct gcggcgacgc taatcgcatg gcgaaagacg
     9181 ttgagcaggc acttctggaa gtgattgccg aatttggtgg catggacacc gaagcggcgg
     9241 atgaattttt aagtgagctg cgcgtagagc gccgttatca gcgagatgtc tactaatgag
     9301 cgaaaaacat ccagggcctt tagtggtcga aggaaaactg acagacgccg agcgcatgaa
     9361 gcttgaaagc aactacctgc gcggcaccat tgcggaagat ttaaacgacg gcctgaccgg
     9421 cggctttaag ggcgataact tcctgctgat ccgcttccac ggcatgtatc agcaggatga
     9481 ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag ccgcgccatg cgatgctgtt
     9541 gcgctgccgt ctgccgggtg gagttatcac caccaaacag tggcaggcga tcgataaatt
     9601 tgccggtgaa aacaccatct atggcagcat tcgcctgacc aaccgccaga cattccagtt
     9661 ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag atgctgcact cggtcggtct
     9721 tgatgcgctg gcgaccgcta acgacatgaa ccgtaacgta ctctgcacct cgaaccctta
     9781 cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag aaaatctctg aacatctgct
     9841 gcctcgtacc cgcgcgtatg cggagatctg gctcgatcag gaaaaagtcg ccaccaccga
     9901 tgaagaaccg atcctcggcc agacctacct gccgcgtaaa ttcaaaacca cggtagtgat
     9961 cccgccacag aacgatatcg atctgcacgc caacgacatg aacttcgtgg caatcgccga
    10021 aaacggcaag ctggtgggct ttaacctgtt ggtgggcggt ggactttcta tcgaacacgg
    10081 caacaagaaa acctacgccc gcacggcgag tgagtttggc tatctgccgc tggagcatac
    10141 gctggcagtg gcggaagccg tcgtgacaac tcagcgtgac tggggtaacc gaaccgatcg
    10201 taaaaatgcc aaaaccaaat acacgctgga gcgcgtgggg gttgagacgt ttaaagcgga
    10261 agtggagcgt cgcgcgggga tcaaatttga accgatccgt ccgtatgagt tcaccggacg
    10321 cggagatcgt attggctggg ttaagggcat tgatgataac tggcacctga cgctgtttat
    10381 cgaaaatggt cgcatccttg attatccggg gcgtccgctg aaaaccggcc tgctggagat
    10441 cgcgaagatc cacaaaggtg atttccgcat tacggcgaac cagaatctga tcatcgccgg
    10501 tgtgccggaa agcgagaaag cgaagatcga gaagatcgcc aaagagagcg ggttaatgaa
    10561 tgccgtcacg ccgcagcgtg aaaactcaat ggcctgcgtc tcgttcccga cttgcccgct
    10621 ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc gacaacatcg ataatttaat
    10681 ggcgaaacat ggtgtcagcg atgagcatat cgtgatgcgt gtaacaggct gcccgaacgg
    10741 ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt aaagcgccgg gtcgctacaa
    10801 cctgcatctt ggcggcaacc gcattgggac acgtatccca cggatgtata aagaaaacat
    10861 caccgagccg gaaatcctgg cgtcgcttga tgaactgata gggcgctggg cgaaagagcg
    10921 cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg ggcatcattc gcccggtgct
    10981 cgatccggcg cgcgatttgt gggattaacc atcagcccgg tcttgtaggc ctgataagac
    11041 gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc tatgtccaaa ctcgatctaa
    11101 acgccctgaa cgaactgccg aaggtagatc gcattctggc gctggcggaa actaacgccc
    11161 aactggaaaa actggacgct gaaggccgcg tagcctgggc gctggataat ctgcccggtg
    11221 aatatgtact ttcttccagc tttggcattc aggcggcggt gagcctgcat ctggtgaatc
    11281 aaattcgccc ggatattccg gtgatcctca ccgatacggg ttacttgttc ccggaaacct
    11341 accgctttat tgacgagtta acggacaaac tcaagctcaa cctgaaagtg taccgtgcta
    11401 ccgaaagcgc agcctggcag gaagcacgct acggaaaact gtgggaacag ggcgttgaag
    11461 gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat gaaccgggcg ctgaaagaac
    11521 ttaacgcgca aacctggttt gctggcctgc gccgtgaaca atccggcagt cgcgccaatt
    11581 taccggtgct ggcaattcag cgtggcgtat ttaaagtgct gccgattatc gactgggata
    11641 accgaacgat atatcagtat ctgcaaaaga atggcctgaa atatcaccca ttatgggacg
    11701 aaggatattt atcggtgggc gatacccata caacccgtaa atgggaaccc ggcatggcgg
    11761 aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg gttacacgaa gggtaaatat
    11821 tgccggataa attaaacgcc tctgtcagta atgatggggg cgtttttatt ttgcgagcag
    11881 cattcctgat tttaaacgtg aacgccgctg gaagaattat caggtataga atggcgtcgg
    11941 gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc agatggacaa aagccccagg
    12001 cgatatttct atcaacctga ggctcgcgtt cgaacgtagc aattcggatg ttagtctctt
    12061 ctttgcaggg aggcaagaca tgctaacaaa atatgccctt gtggcaatca tagtactgtg
    12121 ttgtacagta ctgggattca cgctcatggt aggcgactcg ttatgtgagt tgagtatcag
    12181 agagcgtggt atggagttta aggcagttct cgcttacgaa tcgaagaagt agccaccacg
    12241 cgggggtaaa accccgcgta ttgaattgtg atggtttggt ctcctgcacc cttttttaaa
    12301 tttatatttt ttatgtgaat atatagtctc tatttatcat tatgaatttc aaatgaaaat
    12361 cattaattta ttcataatat aaatgtttat tggctgatat atatcacaga taatttttaa
    12421 aa
//
LOCUS       DAZFBR010000125        12055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFBR010000125 DAZFBR010000000
VERSION     DAZFBR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.125"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(56..991)
                     /gene="rihA"
                     /locus_tag="ACHJZ2_004027"
     CDS             complement(56..991)
                     /gene="rihA"
                     /locus_tag="ACHJZ2_004027"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO4570049.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHAPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(1109..1834)
                     /gene="gltL"
                     /locus_tag="ACHJZ2_004028"
     CDS             complement(1109..1834)
                     /gene="gltL"
                     /locus_tag="ACHJZ2_004028"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO4570050.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(1834..2508)
                     /gene="gltK"
                     /locus_tag="ACHJZ2_004029"
     CDS             complement(1834..2508)
                     /gene="gltK"
                     /locus_tag="ACHJZ2_004029"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO4570051.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(2508..3248)
                     /gene="gltJ"
                     /locus_tag="ACHJZ2_004030"
     CDS             complement(2508..3248)
                     /gene="gltJ"
                     /locus_tag="ACHJZ2_004030"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO4570052.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(3418..4326)
                     /gene="gltI"
                     /locus_tag="ACHJZ2_004031"
     CDS             complement(3418..4326)
                     /gene="gltI"
                     /locus_tag="ACHJZ2_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO4570053.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            complement(4723..6261)
                     /gene="lnt"
                     /locus_tag="ACHJZ2_004032"
     CDS             complement(4723..6261)
                     /gene="lnt"
                     /locus_tag="ACHJZ2_004032"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO4570054.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQS
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(6286..7164)
                     /gene="corC"
                     /locus_tag="ACHJZ2_004033"
     CDS             complement(6286..7164)
                     /gene="corC"
                     /locus_tag="ACHJZ2_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO4570055.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(7254..7721)
                     /gene="ybeY"
                     /locus_tag="ACHJZ2_004034"
     CDS             complement(7254..7721)
                     /gene="ybeY"
                     /locus_tag="ACHJZ2_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO4570056.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(7718..8758)
                     /locus_tag="ACHJZ2_004035"
     CDS             complement(7718..8758)
                     /locus_tag="ACHJZ2_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO4570057.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(8911..10335)
                     /gene="miaB"
                     /locus_tag="ACHJZ2_004036"
     CDS             complement(8911..10335)
                     /gene="miaB"
                     /locus_tag="ACHJZ2_004036"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO4570058.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            10481..11656
                     /gene="ubiF"
                     /locus_tag="ACHJZ2_004037"
     CDS             10481..11656
                     /gene="ubiF"
                     /locus_tag="ACHJZ2_004037"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO4570059.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(11810..11884)
                     /locus_tag="ACHJZ2_004038"
     tRNA            complement(11810..11884)
                     /locus_tag="ACHJZ2_004038"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11850..11852),aa:Gln,seq:ctg)
     gene            complement(11922..11996)
                     /locus_tag="ACHJZ2_004039"
     tRNA            complement(11922..11996)
                     /locus_tag="ACHJZ2_004039"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11962..11964),aa:Gln,seq:ctg)
BASE COUNT         2864 a         3141 c         3170 g         2880 t
ORIGIN      
        1 taaacagatt aagaccaccc aggtaacgcg tggtgatctt aatcaatgac gtgtgttaag
       61 cgtaaaattt cagacgatca gccagtaaat caacaaagcc ctgacgatca acatcgacca
      121 ttacagtggc attcggttta ttgccagtca gataataata atcaacaacc gtcataccct
      181 gggtatattt cccctgtgtt tccacgccaa cccagcgctc aacagtggta aacaactccg
      241 gttttaacag ccaggcgatg gtgcatgggg catgtagcgg tgcgccgaca aagccccatt
      301 tttcgtcttt atgatattcg aggaagaaat ccagcagttc ggcaacaatg gttgaaacag
      361 ggttgccaat cgcgcggaaa cgctcagtgt cttcaacgtg gatttgtgct ttgtgagtaa
      421 catccagacc ggccatcacc accgggatcc ctgactggaa gacaatttct gccgcttccg
      481 ggtcaacgta aatattaaat tcagccgcag gcgtccagtt acccagtccc atagcgccac
      541 ccataatcac gatacgggca attttgctat gcagttccgg atggctattg agcagcaagg
      601 caacgttagt ttgcggtccg gtagacacaa tcgtgacagg ttccgcactt tcacgcagcg
      661 ttttcgccat cagctctacc gccgtacagt tttgcggtgc gaacgtcggt tccggtaatg
      721 ccgggccgtc gagaccgctt tcgccgtgca cattgtccgc gataatcaac tcacgcatta
      781 acggttttac cgcgccgcct gccaccggaa tatcggtgcg attaagcaag gtcagcatac
      841 gcagaacatt gcgtaaggtt ttttctggtg tctggtttcc ggcggaagac gtaattgctt
      901 tgacatcaag ctctggtgag gcgagggcga gaactattgc gatagcgtca tcatgacctg
      961 ggtcgcaatc taacagaatt ggcagtgcca ttgttgctcc ttgttgtgtg cttctttgcg
     1021 acaagggtaa cgccaggatg taacagatac gaggggcgag acgataaagc gtgagatggc
     1081 gcgcacttgg gtatgcgcgc cagagtgatt aatgcaggat tttcgcgagg aagtcttttg
     1141 cgcggtccga tttcggatca tcgaagaacg catctttcgg cgagtcttca acaattttac
     1201 cctcatccat aaagatcacc cgattcgcca ctttacgggc aaagcccatt tcgtgggtca
     1261 ccaccatcat ggtcatcccc tcgttcgcca gttccaccat cacgtccagt acttcgttga
     1321 tcatctccgg gtctagtgcc gatgtgggtt cgtcgaacag catcgcaata ggatccatac
     1381 acaatgcgcg agcaattgcc acacgctgct gctggccgcc ggaaagctgc gccggaaatt
     1441 tattggcatg tgcggaaagt cccacgcgct ccagcaattt caatgctttt tcacgagccg
     1501 gtgctttatc gcgtttaagc actttcacct gcgccagggt caggttttcg ataatcgaca
     1561 gatgagggaa cagctcgaaa tgttggaata ccatcccgac gcgagaacgc agctttgcca
     1621 gatcggtttt cttgtcgtta accacgatac catcgacggt gatttcacct tgctgcactg
     1681 gttcgaggcc gttgacggtt ttaatcagcg ttgatttgcc ggaaccagac gggccgcaaa
     1741 ccaccaccac ttcgcctttt ttcacttcgg ttgagcagtc ggtcagcacc tgaaagtgac
     1801 cataccattt tgaaacattt ttcagggtaa tcattatgct gtccttcttt tcaagtagct
     1861 gaccaacaac gacgcgctaa ggctaataac gaaataaaca aatccggcaa acaggatcat
     1921 ctcaacctgc gtgccatcac gctcaccaat ggttgaggcg gtacggaaga aatcggccag
     1981 agataacaca tacaccagcg aggtatcctg gaacagtacg atgccctgag tgagcagcag
     2041 cggcaccatc gcgcggaacg cctgcggcag aataatcagt ttcatcgact gccagtgagt
     2101 cattcccaac gccagcgcgg cgctcgactg accacgagaa atactttgaa taccggcacg
     2161 gataatctct gaatagtagg ccgcttcaaa catcgaaaac gccaccatcg ccgaaattaa
     2221 acggatatca ttttttggtg ataatcccag cacgttttgc agaaaacccg gcacgatcag
     2281 gtaaaaccac agcaaaacca taactaaagg aatcgagcgg aatacgttaa cgtaggcttt
     2341 ggcaaaccac gctacgggcg caaagctgga caagcgcatc accgccagca tggtgcccca
     2401 caaaatacca atcacgactg ccgtgacggt gattttcagc gtgatcacca gcccgtcgag
     2461 cagatatggc agggaaggga caatggaact ccagtcaaac tcgtacatta tttgcccccc
     2521 atgttgccag gcaggcgaac tttacgttca accagcgtca tcaccagcat gataaaagcg
     2581 ttaataaaca catacgctag cgtaatggcg gtaaacgact cccaggcgtg agccgagtaa
     2641 tcgagcaatt tactcgcctg cgctgccata tctaccagac cgatggtcga ggcgatggcg
     2701 gagtttttca ccaggttcat catctctgag gtcatcggcg ggacgataac gcgataagca
     2761 ttaggcagca gtacgtagcg gtaagcctgc ggcagcgtca gacccatagc cagcgcggca
     2821 ttcttctgcc cacgcggcag cgactgaatc gcggcgcgta cctgttcgca aacacgggcg
     2881 gcggtaaaca gccccaggca gagcatggat gaaaggaaaa actgaatatt gggatccagc
     2941 tcggccttaa accacatgcc gattttctcc ggcagcagct ccgggatcac cagataccag
     3001 gtaaagaatt gcacaatcaa cggcacgttg cggaacagtt cgacatacaa cgtacccaga
     3061 ccggagagaa aacggtttgg aacggtacgt aaaataccga aaaatgaacc gacgaggaaa
     3121 gcgataatcc aggcgcagat cgacaaagcg atcgtcacct gaaaaccact ccagatccaa
     3181 ccaagatagg tggtgttgcc gaacggggct tgttgtaaaa aaatacccca gttccagtct
     3241 atagacataa atctactcca gaaaaaagag ggtagcagcg ttaactgcta ccctcgaaga
     3301 ttgttaccca gcgtattgcg gttttcaggc ccgatgggga acgaccatca ggcgtatagt
     3361 ctgtccgtgc tacgtaacaa tcgagagggc tagaatttcc gcccctggtt cttgtaatta
     3421 gttcagtgct ttgtcattcg gttctttgaa cagtgctttc atttcgtctg acagttcgaa
     3481 attcatgttc aggtttttcg gcggaatggg atttttgaac catttatcaa accatttttc
     3541 cgcttcaccg gaggtctgca cctgagcgat agtgtcatcc atcagctttt tgaactgcgg
     3601 atcgtcttta cgcaacatgc aaccataggc ttcctgagac tgcggcttgc cgacgatatc
     3661 ccagttgtct ggtttcttcg ctttcgcacg ttcaccggcc agcagagcat catccatcat
     3721 aaaggcaacg gcacgaccgc tttccagggt acggaaagag tcaccgtgat ctttggcgct
     3781 gatgatgcgc atattcattt tttgctcttc attcagtttg ttgagcaaaa cttcagaggt
     3841 agtgccggaa gtgacgacta cggctttgcc tttcaggtcg gcaaaatctt tgatatcgcc
     3901 gccctttttg gtcaacaggc gcgtaccgac cacgaaaata gtgtcagaga aagccgcctg
     3961 tttttggcgt tcgacgttgt tggtggtaga accacattca aaatcaaaag tgccgttttg
     4021 cagcagcgga atacggtttt gtgaggtaat cggaatcagt ttcacctgca agtccggttt
     4081 gttgagtttc tttttcactg cttcaacaat ggcgttggag taatcctgcg aataacccac
     4141 cactttttgc tgattgtcgt aataagagaa aggtactgaa gattcacggt gaccgacgac
     4201 aatcacgccg tttttggcga ttttgtccag cgtactgccc gctgccgggg cggcgtcatc
     4261 tgcctgtgcc agtcctgcgg aaagcgccag ggccaggatt gctgtggcag gtttacgtaa
     4321 ttgcatatcc aactccttta tcttctgcgt taagaacgca tggatacccg ttgtgagtgt
     4381 ttgtgttgtt atcatctgca actttattgt gtagtgttgt gcctgttagg taacatttag
     4441 tttggctaaa tgtaaagata ttgctgtttt attgtttata tttgggaggt gcgccgcacc
     4501 attccgaagc aaaattctta aaatgcactc ttttggtgct accggtggat tgctgtggtg
     4561 caaccgggtt gtactgatgc tggttaaagc tttgccttgt ataacaaagc aatagatgtg
     4621 ccaaagttgg ataggagaat attgttatcc ggataatgca ctgatgccgc atccggtgag
     4681 cgtgccgaaa tatgggatgt attccggcac gataagaagg gattatttac gtcgctgacg
     4741 cagactcatc aacacagcag caaaaccaaa cagtgccgtc agcacccaca gcggccagtt
     4801 gccggtacgt gcgtatggtg tgagtccggt ggtcggcgtc acgttagtgg ttaacacctc
     4861 gcgggtgaac tgcgggatca tcgcctgaat ctcaccctgc gggccaatca ccgccgtaat
     4921 gccgttgttg gtgctgcgca acagtgggcg cgccagctcc agcgcacgca ttcgcgccat
     4981 ctggaagtgc tgccatggac caatagactt accaaaccac gcatcgttgg agatagtcag
     5041 cagatagtcg gtatccgggc ggaagttatc gcgcacttgc tcgccgagaa tgatctcgta
     5101 gcaaatagcc gcagtaagct caataccatt tgccgacagc ggcggctgga tatatggccc
     5161 acggctgaac gacgacatcg gcagatcaaa gaacggtgct aacggacgca gaatcgactc
     5221 cagcgggaca aactcgccaa acggcaccag atggtttttg ttatagcgat cggctgattc
     5281 gtagctgtac ggtgcacctt tacccagtgt gatgatggtg ttgtaggtat cgtaacggtt
     5341 ctgcttatta agacgcgcgt caacaatccc ggtcaccagc gagctacctt tatcacgcaa
     5401 ctcaccgtcc agtgctttga ggaacggttg ctgattaatt tccagatcgg ttatcgccga
     5461 ctccggccag ataatcaacg atgatttgcc catcagcggt gccgttgcgt tgtagtaaat
     5521 cttcagcgta ttaagaagct ggccttcgtc ccatttcagc gattgcggaa tatcgccctg
     5581 aaccatcgaa acctgaatgg ttttctccga ttgtggggta aaccaccgga tgtaacgcag
     5641 cgggaaggga agggcaaaca gcacgacggc caccaccagc ggacgccagt tgcgtttgac
     5701 taacgccagt gccagcaggc cgctaaccat cataagcagg aagttaatgg cttccacgcc
     5761 cattagcggt gccagccctt ttaacgggcc atcaatctgg ctatagccga actgtaacca
     5821 cgggaagcct gtcagtaccc aaccgcgcag aaactcggtc acttgccaga gagcaggggc
     5881 ggcaatcgcc acgcgcagcc aggtggtttt cggccacagg cgcgacagca cgccagcaaa
     5941 cagtccggta tacagcgaca aatacgccgc cagcagtacc accaggaaga tgttaaccgg
     6001 gccaggcatt ccgccaaagg tcgcgatgct gacatagacc cagttaatac cgctgccaaa
     6061 gaggccaaat ccccagcaaa agccaatagc ggcagactgg agtggacggc ggttaaaggt
     6121 caacgcctga agccccatca gcgaaataat cgccgcaggc cagacgtcgt aaggagagaa
     6181 ggccagcgtt ccgcaggcac cgaataataa cgccagcagc aggcgaatgc gctggcgttc
     6241 aattaatgag gcaaaagcca tgtagttatc tatccagttt cggttttatt catccagctt
     6301 cggctggggt gagtcatccg ggattttgac atgaacctga ataatacgcc gactgtcggc
     6361 catcgccact ttgaactggt aaccgtcgat gtcgatggtt tcgccacgtg ccggaagatg
     6421 cccaaatgcc tgcatcacca gaccaccgat agtgtcgact tcttcatcgc taaagtgggt
     6481 gccgaacgct tcgttgaagt cttcaatgga agccagtgcg cgcacggtcc aggtatgacg
     6541 actcagctga cggaagtcga tatcatcttc ttcgtcatat tcgtcttcaa tctcaccaac
     6601 aatcagttcc aggatgtctt caatggttac cagaccggaa accccaccga attcgtcaat
     6661 aacgatcgcc atgtggtaac gctgagagcg aaactctttc agcatccggt ctacgcgctt
     6721 actttcagga acgacaaccg cctgacgtaa cactttgtcc atgctgaagg cttcagcatc
     6781 gctgcgcata aacggcagca agtctttcgc catcagaatc ccttcaatgt gatctttgtc
     6841 ttcgctaatc accgggaaac gtgagtgggc ggactcgatg atgacatcaa ggcattcgtc
     6901 cagcgtctgg ttgcgtttca gggtaatcat ctgggagcgg gggatcatga tgtcgcggac
     6961 gcgttggtct gcgatgtcca tcaccccttc gagcatatcg cgcgtatctt cgtcgataag
     7021 gtcgttctgc ccggaatcac ggatcagcgc cagcagttcg tcacggtttt tcggttcacc
     7081 gtggaaaagt tggctgagta acagggagaa aaatcccttc ttgttgctta tcgtgtcact
     7141 actgtgtgaa ttgtcgtcgc tcatggcgtc gtatgggttc tcatgttagt taatcaaaac
     7201 gccgtcgtta atcaccaacg gcggggacgt ctgccagtca aatggctggc aaattattct
     7261 ttctcggcaa tgtacggatc ctcatagccc agagcaagca taatctctgt ttcgagggct
     7321 tccatttctt ctgcttcgtc atcttcgatg tgatcgtaac ctaacaaatg cagactgccg
     7381 tgcaccacca tatgcgccca gtgcgcctcc agtggtttgc cttgctcctg agcttccttc
     7441 tcaaccatct gacggcagat aaccagatcg cccagtagcg acatctccat gccaggcggc
     7501 acttcaaacg ggaaggagag cacgttggtc ggcttatcct taccgcgata ggtcagattg
     7561 agactgtggc tttcggcggt atcgaccacg cgaatcgtca cttccgactc ttcctgaaac
     7621 tgcgggatca ccgcattcag ccatgtttga aactggctct cttccggcaa tccggagtta
     7681 tcttcacatg ccagttgtaa atcgaggatc acctgactca tttttgttcc tgttcttcgc
     7741 gcttgcgttc tgccgccagc gccgctttac gtttttgttc ggcttcttcc caggcttcat
     7801 aggcgttaac gatacgcgcc accaccgggt gacgaaccac gtcttcgctg tggaagaagt
     7861 taaagctgat ctcttcgaca tcggccagca cttcgatggc atgacgtaag cctgatttag
     7921 tattacgcgg caggtcgatc tgtgtgacgt cgccggtgat aaccgctttt gagttaaaac
     7981 cgatacgggt caggaacatc ttcatctgtt cgatggtagt gttctggctc tcatcgagaa
     8041 tgataaatgc gtcgttcagc gtacgaccac gcatataggc cagcggtgcg acttcaataa
     8101 cgttgcgctc aatcagtttc tcgactttct caaaacccag catttcaaac agcgcgtcgt
     8161 acagcgggcg cagatacggg tctactttct ggcttaaatc gccaggcagg aagcccagtt
     8221 tctcaccggc ttctactgcc ggacgagtca gcagaatacg gcgaatttcc tgacgctcca
     8281 gggcatcaac tgccgcagcc actgccaggt aggttttacc cgtacccgcc gggccaacgc
     8341 cgaaggtaat gtcatggtcg agaatattgg cgatgtactg cgcctggttt ggcgtgcgcg
     8401 gcttaattac gccgcgtttg gttttgatat tgaccgcttt gccgtactcc ggcacgctct
     8461 ccgcgctttg ctccagtacc cgtgcttctt taatcgcaag gtggatctgt tccggttcga
     8521 tatcctgaat ctgaccgcgc atcggggcag tatcgacata caggctacgc agaatgtctg
     8581 ccgcagcggt gacgcaaatc ggacggccgg tcagtttaaa gtggttatcg cggcgattga
     8641 tctcgatgcc gagacggcgt tcgagctgct tgatgttgtc atcaaacggg ccgcacaggc
     8701 tcaacagacg cgcgttgtct gctggctcca gggtgatttc gcgagtgtct atgttcaaac
     8761 cgttcctctt atctgtatgc cgccggaagc tgaacattca ccggcctata aggaaattat
     8821 tcacgccaca ggaaaaaggc gcaagcattg cgataaagat ggggataaag agagaaaaaa
     8881 caaggcccac cggaacggcg ggcctgagaa ttacggctga taataaccca cgccaaggtc
     8941 gttttctttg cgagtacggg caatcaccga ttctggtgtt tctgccacgc gcagacccat
     9001 ttcatcttca gtacgcacca ctttaccgcg cagagagttc gggtagacgt cggtaatttc
     9061 tacatcgacg aatttaccga tcatatccgg cgtgccttcg aagttgacca cgcggttatt
     9121 ttccgtacgc ccggaaagct ccatgatgct cttacgcgat gtaccttcta ccagaatacg
     9181 ctgggtggtg ccgagcatcc ggcggctcca cgccatcgct tgctgattga tacgttcttg
     9241 cagaatatac agacgctgct tcttctcttc ttccggaaca tcatcaacca tatcggcggc
     9301 tggtgtaccc ggacgtgcag agaagataaa gctgtagctc atgtcgaaat tgacgtcggc
     9361 aatcagcttc atcgttttct cgaagtcttc ggtggtttcg ccagggaagc cgacgatgaa
     9421 gtcagaactg atctgaatat ctggacgcgc cgcacgcagt ttacggatga tcgctttgta
     9481 ctccagcgcc gtatgggtac gtcccatcag gttcagaatg cgatcggaac cgctctgtac
     9541 cggcagatgc aggaagctca ccagctccgg cgtgtcgcga tacacttcga tgatatcgtc
     9601 ggtgaattcg atcggatggc tggtggtaaa gcgaatacga tcgatcccgt cgatcgcagc
     9661 aaccagacgc aacagatcgg caaacgatcc ggtggtgccg tcgtagtttt caccacgcca
     9721 ggcgttcacg ttctgaccga gcaggttgac ttcacgcacg ccctgagccg caagctgggc
     9781 aatctcaaac agaatatcgt cagacggacg gcttacctct tcaccacggg tgtaaggcac
     9841 cacgcagtag gtgcaatatt tattgcagcc ttccatgatg gagacaaacg cggtcggccc
     9901 ttcggcgcgc ggttccggta gacggtcaaa cttctcgatt tccgggaagc tgatatctac
     9961 aaccgggctg cggtcgccgc gcacggagtt gatcatctcc ggcagacggt gcagcgtttg
    10021 cggcccaaaa ataatatcga catagtgggc gcgctggcga atgtgctcgc cttcttgcga
    10081 tgccacgcag ccaccgacgc cgataatcag gtctggattc ttctctttta acagtttcca
    10141 gcgacccaac tgatggaaga ctttttcctg agccttctcg cggattgagc aggtgttcag
    10201 cagcagcaca tccgcttctt ccgccacgtc ggtcagttga tagccgtggg tggcatccag
    10261 cagatcggcc atcttcgatg aatcgtactc gttcatctga cagccccagg ttttaatatg
    10321 gagttttttg gtcatcgact tgctcttgcg aaatagtagc caggaatgca gggcgcatag
    10381 tgtaatgctt tgctgccgtt gtgaccagta tgagcgttat cagcccttgg gggtaaaaat
    10441 cctgtaaact taaagcatat tgctaacagg atgattgacc atgacaaatc aaccaacgga
    10501 aattgccatt gtcggcggag gaatggtcgg cggcgcactg gcgctggggc tggcacagca
    10561 cggatttacg gtaacggtga tcgagcacgc agaacctgca ccgtttgtcg ctgatagcca
    10621 accggacgtg cggatctcgg cgatcagcgc ggcttcggta tcattgctta aagggttagg
    10681 ggtctgggat gcagtacagg ctatgcgttg ccatccttac cgcagactgg aaacgtggga
    10741 gtgggaaacg gcgcatgtgg tatttgacgc agccgaatta aagttgccgt tgcttggcta
    10801 tatggtagaa aacactgtcc tgcaacaggc attgtggcag gcgctggaag cgcatccgaa
    10861 agtaacgtta cgtgtgccaa cctcgctgat tgcattacat cgagataatg atcttcagga
    10921 gctggagctg aaaggcggtg aagtgattcg cgcgaagctg gtgattggtg ccgacggcgc
    10981 aaattcgcag gtgcggcaga tggcgggaat tggcgttcat gcatggcagt atgcgcagtc
    11041 gtgcatgttg attagcgtcc agtgcgagaa cgatcccggc gatagcacct ggcagcaatt
    11101 taccccggat ggaccgcgcg cgtttctgcc gctgtttgat aactgggcat cgctggtgtg
    11161 gtacgactct ccggcgcgca ttcgccagtt gcagaatatg aatatggcgc agttacaggt
    11221 ggaaatcgcg aagcatttcc cgtcgcgtct gggttacgtt acaccgcttg ccgctggtgc
    11281 gtttccgctg acacgccgcc atgcgttgca gtatgtgcag ccggggcttg cgctggtggg
    11341 tgatgccgcg cacaccatcc atccgctggc ggggcagggg gtgaatcttg gttatcgtga
    11401 tgtcgatgcc ctgattgatg ttctggtgaa tgcccgcagc tacggcgaag cgtgggccag
    11461 ttatcctgtc ctcaagcgtt accagatgcg gcgcatggcg gataacttca ttatgcagag
    11521 cggtatggat ctgttttatg ccggattcag taataatctg ccgccgctgc gttttgtgcg
    11581 taatcttggt ttgatggcgg cggagcgtgc tggcgtgttg aaacgtcagg cgctgaaata
    11641 tgcgttaggg ttgtagcctt acaacattgc cggatgcgtg ccaaccgtag gtcggataag
    11701 acgcggcagc gtcgcatccg acattgaagg ataagacgtg tcaacatcgc attcgacatt
    11761 gagtgaacgc agaaaagcaa aaagctcgcc gaagcgagct tttttaatgt ggctggggta
    11821 cgaggattcg aacctcggaa tgccggaatc agaatccggt gccttaccgc ttggcgatac
    11881 cccaactggg tgcacttaca aggtaagcgt cttgaataaa ttggctgggg tacgaggatt
    11941 cgaacctcgg aatgccggaa tcagaatccg gtgccttacc gcttggcgat accccaacaa
    12001 attggttttt gaatttgccg aacgtattcg acacattcag aatttggtgg ctacg
//
LOCUS       DAZFBR010000126        11958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFBR010000126 DAZFBR010000000
VERSION     DAZFBR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.126"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(52..960)
                     /gene="melR"
                     /locus_tag="ACHJZ2_004040"
     CDS             complement(52..960)
                     /gene="melR"
                     /locus_tag="ACHJZ2_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO4570060.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1243..2598
                     /gene="melA"
                     /locus_tag="ACHJZ2_004041"
     CDS             1243..2598
                     /gene="melA"
                     /locus_tag="ACHJZ2_004041"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO4570061.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2701..4122
                     /gene="melB"
                     /locus_tag="ACHJZ2_004042"
     CDS             2701..4122
                     /gene="melB"
                     /locus_tag="ACHJZ2_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO4570062.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(4261..4890)
                     /locus_tag="ACHJZ2_004043"
     CDS             complement(4261..4890)
                     /locus_tag="ACHJZ2_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO4570063.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(5013..6659)
                     /gene="fumB"
                     /locus_tag="ACHJZ2_004044"
     CDS             complement(5013..6659)
                     /gene="fumB"
                     /locus_tag="ACHJZ2_004044"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4570064.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            complement(6737..8077)
                     /gene="dcuB"
                     /locus_tag="ACHJZ2_004045"
     CDS             complement(6737..8077)
                     /gene="dcuB"
                     /locus_tag="ACHJZ2_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO4570065.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(8648..9367)
                     /gene="dcuR"
                     /locus_tag="ACHJZ2_004046"
     CDS             complement(8648..9367)
                     /gene="dcuR"
                     /locus_tag="ACHJZ2_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO4570066.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(9364..10995)
                     /locus_tag="ACHJZ2_004047"
     CDS             complement(9364..10995)
                     /locus_tag="ACHJZ2_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO4570067.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDRERSNR"
     gene            11176..11406
                     /gene="yjdI"
                     /locus_tag="ACHJZ2_004048"
     CDS             11176..11406
                     /gene="yjdI"
                     /locus_tag="ACHJZ2_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO4570068.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            11418..11690
                     /locus_tag="ACHJZ2_004049"
     CDS             11418..11690
                     /locus_tag="ACHJZ2_004049"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4570069.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT         2896 a         2852 c         3097 g         3113 t
ORIGIN      
        1 tgcttattat cgacggctaa actatttttt tggctgatac tgatatcgtc tttagccggg
       61 aaacgtctgg cggcgctgtt ggctaagttt gcggtattgt tgcggcgaca tgccgacata
      121 tttgccgaac gtgctgtaaa aacgactact tgaacgaaag cctgccgtca gggcaatatc
      181 gagaatactt ttatcggtat cgctcagtaa cgcgcgaacg tggttgatgc gcatcgcggt
      241 aatgtactgt ttcatcgtca attgcatgac ccgctggaat atccccattg catagttggc
      301 gttaagtttg acgtgctcag ccacatcgtt gatggtcagc gcctggtcat agttttcggc
      361 aataaagccc agcatctggc taacataaaa ttgcgcatgg cgcgagacgc tgtttttgtg
      421 tgtgcgcgag gttttattga ccagaatcgg ttcccagcca gagaggctaa atcgcttgag
      481 catcaggcca atttcatcaa tggcgagctg gcgaatttgc tcattcggac tgtttaattc
      541 ctgctgccag cggcgcactt caaacgggct aagttgctgt gtagcaagtg atttgatcac
      601 catgccgtga gtgacgtggt taatcaggtc tttatccagc ggccaggaga gaaacagatg
      661 catcggcaga ttaaaaatcg ccatgctctg acaggttccg gtatctgtta gttggtgcgg
      721 tatacaggcc cagaacagcg tgatatgtcc ctgattgata ttcacttttt cattgttgat
      781 caggtattcc acatcgccat cgaaaggcac attcacttcg acctgaccat gccagtggct
      841 ggtgggcatg atatgcggtg cgcgaaactc aatctccatc cgctggtatt ccgaatacag
      901 cgacagcggg ctgcgggtct gtttttcgtc gctgctgcac ataaacgtat ctgtattcat
      961 ggatggctct ctttcctgga atatcagaat tatggcagga gtgagggagg atgactgcga
     1021 gtgggagcac ggttttcacc ctcttcccag aggggcgagg ggagtgtccg agcatcatga
     1081 ggccgaaaac tctgcttttc aggtaaatta ttcccataaa ctcagattta ctgctgcttc
     1141 acgcaggatc tgagtttatg ggaatgctca acctggaagc cggaggtttt ctgcagattc
     1201 gcctgccatg atgaagttat tcaagcaagc caggagatct gcatgatgtc tgcacccaaa
     1261 attacattta tcggcgctgg ttcgacgatt ttcgttaaaa atattcttgg tgatgtgttc
     1321 catcgcgagg cgctgaaaac ggcgcatatt gccctgatgg acattgaccc cacccgcctg
     1381 gaagagtcgc atattgtggt gcgtaagctg atggattcag caggggccag cggcaaaatc
     1441 acctgccaca cccaacagaa agaagcctta caggatgccg attttgtcgt ggtggcattt
     1501 cagattggcg gttatgcacc ttgcacggtg actgatttcg aggtctgtaa gcggcatggt
     1561 ctggaacaaa ccattgccga tacgttgggg ccgggcggta ttatgcgcgc gctacgtacc
     1621 attccgcatc tgtggcaaat ttgcgaggac atgacggaag tctgccccga tgccaccatg
     1681 ctcaactatg ttaacccaat ggcgatgaat acctgggcga tgtatgcccg ctatccgcat
     1741 atcaaacagg tcgggctgtg ccattcggtg cagggaacgg cggaagagct ggcgcgtgac
     1801 ctcaatatcg acccggctac gctgcgttac cgttgcgcag gtatcaacca tatggcgttt
     1861 tacctggagc tggagcgcaa aaccgccgac ggcagttacg tgaatctcta cccggaactg
     1921 ctggcggctt atgacgcagg gcaggcaccg aagccgaata ttcacggcaa tactcgctgc
     1981 cagaatattg tgcgctatga aatgttcaaa aagctgggct atttcgtcac ggaatcgtca
     2041 gaacattttg ctgagtacac accgtggttt attaagccag gtcgtgagga tttgattgag
     2101 cgttataaag taccgctgga tgagtacccg aaacgctgcg tcgagcagct ggcgaactgg
     2161 cataaagagc tggaggagta taaaaacgcc tcccggattg atattaaacc gtcacgggaa
     2221 tatgccagca caatcatgaa cgctatctgg acgggcgagc cgagtgtgat ttacggcaac
     2281 gtccgtaacg atggtttgat tgataacctg ccacaaggat gttgcgtgga agtggcctgt
     2341 ctggttgatg ctaatggcat tcagccgacc aaagtcggta cgctaccttc gcatctggcc
     2401 gccctgatgc aaaccaacat caacgtacag acgctgctga ccgaagctat tcttacggaa
     2461 aatcgcgacc gtgtttacca cgctgcgatg atggacccgc atactgccgc cgtgctgggc
     2521 attgacgaaa tatatgctct tgttgacgac ctgattgccg cccacggcga ctggctgcca
     2581 ggctggttgc accgttaaaa cgcgactaaa cgctactgcg ccgggggatt tattccggcg
     2641 cacacctctg acgataccaa taacagaagg cgggcgttgg taacagcgac ccgataccct
     2701 atgagcattt caatgactac aaaactcagt tatggatttg gagcgttcgg gaaggatttt
     2761 gcgatcggca ttgtgtatat gtacctcatg tattactaca ccgatgtcgt cgggctgtct
     2821 gtgggtttgg tcggtacttt gtttctggtg gcgaggatct gggatgctat taacgatccg
     2881 attatgggat ggattgtaaa tgctacgcga tcgcgatggg gtaagttcaa accctggatc
     2941 ctgatcggta cgttggcaaa ctctgtaatc ttatttctcc tctttagtgc gcatctgttt
     3001 gaaggtacta ctcagattgc ctttgtttgc gtgacctaca tcctctgggg catgacttac
     3061 accattatgg atattccctt ctggtcgctg gttccaacca tcacgctcga taaacgtgag
     3121 cgtgaacaac tggttcctta tccgcgtttt tttgccagtc tggcgggctt tgttacggca
     3181 ggtgtgacgc taccatttgt taattatgtc ggcggtggcg atcggggatt tggctttcag
     3241 atgttcacac tggtactgat cgcctttttt attgtttcaa ccatcatcac tctgcgcaat
     3301 gtgcatgaag tcttttcgtc agacaatcaa ccgtctgctg aaggaagcca tctgacactt
     3361 aaagccatcg ttgcgcttat ttataaaaac gatcagcttt catgcctctt gggtatggct
     3421 cttgcttata atgtagccag caacattatt accggctttg ctatctatta tttctcatat
     3481 gttatcggtg atgcggattt gttcccctat tatctgtcgt atgcgggagc tgctaacctg
     3541 gtgacgttag tattcttccc acgcttagtt aaatcattat cccgacgcat tttatgggcc
     3601 ggagcatcta ttcttccggt gttaagctgt ggtgttctcc tgttaatggc attaatgagc
     3661 tatcacaacg tcgtcctcat tgtgattgcg ggtattttgc tgaatgtggg aacggcgctt
     3721 ttctgggtat tacaggtcat catggtggca gataccgttg attacggtga atataaactg
     3781 cacgtacgct gtgaaagcat cgcttactcc gtgcagacta tggtggtgaa gggcggttca
     3841 gcctttgcgg cttttttcat tgcggttgtg ttagggatga ttggctatgt accgaatgtt
     3901 gaacagtcta cgcaagccct attaggtatg cagtttatta tgattgctct accgactctg
     3961 tttttcatgg taacgctgat tctctacttc cgtttctatc gcctcaatgg cgacacgctg
     4021 cgcaggatcc agattcatct gctggataaa tatcgcaaaa taccgcccga gcctgttcat
     4081 gctgatattc cggtcggtgc agtgagtgat gtgaaagcct gacgtggaca atagctaacg
     4141 aaaatggcct gatgcgatat gtttatcagg ccacgtctta atggaatgct ttggatttgc
     4201 aaattttgta gaccggataa ggaattcacg ccgcatccgg catcaacaaa gtgcacgttg
     4261 ttatccggtt atcaagccaa agcgccgtag ctgacggcaa tgaaagcgag cgaggaatat
     4321 caccgtggtt aatgcgccaa gcagcgcgca gaacatatcg gattgcgtgt cccactggtc
     4381 gccctgggtg ccgagaaaat catccgctcc ctggcccatt gccagcgccg cccaccactc
     4441 gattaattca tacatcgcgc ttatcgccag cgccacgcaa cagaccagaa acgccaccat
     4501 tttacgtccg cgaacgtaca tcccgcgcac aaggatctct cgtgccacca gtgcaggcac
     4561 cagcccctgg aaaaaatgcc ccagcttgtc atacggatta cggctcaacc ccagccattc
     4621 ctgcacctca aaaccgatgg ggactttcgc gtaggtgtat tgcccgccga ccatcaggat
     4681 gatggcgtga aagaaaatga gcgtatagag cagcggcgtt aacggataac gtttagcggt
     4741 ggcaagcagt agcggcacga caataatcac cggtgtcact tccatcagcc aggtgagttt
     4801 gtcatgggcc gaaatgccgg tataaatcag gattaacgtt agcgccagtg cgccggtgtt
     4861 aagaattaac ggcttgagtg tgcgggtcat ggtgagttcg caagtcaggg aaaatactga
     4921 ctattcacca gcaagcgtga aattacaatc gcaaaagagg tggcccaggg ggatcacctg
     4981 gcagcatgct gccaggcgct gggccgaaga ggttacttag tgcagttcgc gcactgtttg
     5041 ttgatgattt gctggaagaa gtcgttacct ttgtcatcga ccaggataaa cgccgggaaa
     5101 tcttctactt cgattttcca gatagcttcc atacccagct ccggataagc gacgcattcc
     5161 agatgcttga tgctctgctg cgccagtacc gccgccggac cgccgatgct accgaggtag
     5221 aagccgccgt gtttatgaca cgcgtcggta acctgctgac tgcggttacc tttcgctagc
     5281 atgatcatgc tgccgccgtg ggattgcagc agatccacat aggagtccat acgacctgcg
     5341 gtggttgggc caagtgagcc tgatggataa ccggcagggg ttttcgccgg acccgcgtag
     5401 tagatcgggt gatctttgat gtactgcgga agttctttac cggcgtcaat cagctctttc
     5461 agcttggcgt gtgcaatatc tcggcccacg ataatggtgc cggtgagcga caaacgagtg
     5521 gaaaccggat attgcgaaag ctgggcgagg atctctttca tcgggcggtt aaggtcgacc
     5581 ttcaccgctt cgccttcacc ggcctggcgc agttcttgtg gaatgtactg acctggatgg
     5641 tgttccagtt tttcgatcca gataccttcg cggttgattt tcgctttaat gttacggtca
     5701 gcggagcagg agacgcccat gcctaccggg caggatgcgc cgtgacgtgg cagacggata
     5761 acgcgaatgt cgtgcgcgaa gtatttaccg ccaaactgcg cgccaagacc gagtttctgg
     5821 gcctcttcca gcagttcctg ttccagctgg acatcgcgga acgcctgacc atgttcgttc
     5881 ccttccgtcg gcagttcatc gtaatagtga gcgcttgcta acttaacggt tttcaggttg
     5941 gtttccacag acgtaccgcc aatcacaaac gcgatatggt acggcgggca ggctgcagta
     6001 ccgagggtac gcattttctc gacgaggaag tttttcagtt tgccgggagt cagcagggct
     6061 ttggtttcct ggtagagata ggttttgttg gcagagccgc cgcctttcgc aacgcaaagg
     6121 aatttgtact catcgccatc taccgcgtac aggtcgattt gcgcaggcag gttagtgccg
     6181 gtgttgacct ctttgtacat gtccagcgcc gcgttttgcg agtagcgcag gttatcttcg
     6241 atataggtgt tatagacgcc tttcgacagc gcttcttcat caccgccacc ggtccacacg
     6301 cgctggcctt ttttaccgac gatgatcgcg gtgccggtat cctggcaggt cggcagcacg
     6361 cctttggcgg cgatttcgga gtttcttaag aattgcagtg ccacgtactt gtcgttttcg
     6421 ctggcttctg gatcgtgaag aatagccgca acctgtttct ggtgtgccgg acggagcatg
     6481 aaagaagcgt cgtggaaggc ttgctgcgcc agcagggtca gggcttctgg ttccactttc
     6541 aggatggttt cgccgtcgaa gtcggcaacg ctaacgtaat cggaagtgag tagatagtat
     6601 tcggtattgt ctttccccat cgggaaaggt gcctggtaga taaagggttt gtttgacata
     6661 gcttccagcc tgtaactctg tatttgttat tcgaaaatgg cgtgccgcgt agtaacgggc
     6721 ggcacgcaaa gtgcatttat aagaacccgt acatcgcggc gaagatccag ccgaagacgc
     6781 acgatacgct cacaccaatc aaccccggca gaataaagct gtggttgatg acgaagcgac
     6841 cgatgtgggt ggtgccggaa cggtcaaact gaatcgctgc cagatcgctc ggataagtcg
     6901 gcaggatgta ataaccgtag caagccggtg ctgaagccac gatgtatgcc ggatcaacgc
     6961 cgatcgctag cgcgaccgga acaatcgccg ccagcgccgc agcctgagag tttacaaact
     7021 tggaaaccag cagcagaaca atggcatagg cccacggata ctctttcacc atttcaccca
     7081 gtacgccctg aatttcagac atatgcgcac cgaacatggt ttctgccatc catgcgatac
     7141 cgtacaccgc cacgatggcg atcataccgg aacggaagac ttcgtttttt gagatagacg
     7201 cgggattggt tttggtcagg ataataatca gcgccccggt cagcagcata aacatctgaa
     7261 taaccagtac catcgacagc ggtttgccgc cgaaggatgg acgcaggtcc gaatcagcac
     7321 caagaagtgc gactacagcg attgccccga ggaaaatcca cattgccagc cagttgcttt
     7381 tcggcagttt tttatccagc agcgtcgcgg tatcaccgta aacatactca cggttttccg
     7441 gtacggagat gaatttctgg aactcttcgt ctttatccag atctttaccg cggaaccagc
     7501 tgaagatacc gatcgccagg ataccgatta acgtcgatgg aatggtgatt gccagcaggt
     7561 cgaggaactc aagatggcga ccatcaaagg tgacattacc cagcattgca accagagaca
     7621 cgaccgcaac cgacaccgga ctggcgataa tccccatctg tgcaccgata gaacttgccg
     7681 ccatcggacg ttccggacgg atgttgttct taatggcgac gtcgtagatg atcggcagaa
     7741 tggtgtaaac cacatgaccc gtaccgcaaa gaatggtcag cgtacaggtc acaaacggcg
     7801 cgacaattga gacatatttc gggttgcggc gcagcagctt ctcggcaatt tgcagcatga
     7861 catcaagacc gcccgaagct tgcaaggtcg ccgatgccgc caccaccgca atgataacca
     7921 gcatgacatc aactggtggt ttacctggct gaaggtggaa gacgaagacc agaatgacca
     7981 gaccgatacc gcctaataaa cccagcgcga taccaccctt tctggcacca taaaacagac
     8041 atatcagtat tatgataagt tggatagtaa ataacatgtg tgaaccctcg cgataatcct
     8101 atttaaattt ttgctgaata gatcacagtc acgttctgtt ttgtatgaac tgttttcaga
     8161 gcctgactaa atatccgttt ggtcgttacc ggctttagca aatacctcac agtgaatatt
     8221 ggctgattaa agcagcggca aatttatgca ctgtgataag cggctttttc agagagaaaa
     8281 actctctggc gatgggttta aagtttgtac tgcgcagatg ttaatgtttt gtttgctgtt
     8341 atcttattga tatttatcgg tctatttttt gttctcaggg cagatatcat taggtttata
     8401 gattaatctg atctacccat ttgtgggtaa aaatacacat aatgcgggtg acataatagt
     8461 taattaactt ttgttagcgt tttgaaatta aaaacaccgt tcacctgaag agatattaat
     8521 ttttagcgat gatggaggga taattatatt tgatctggca caagttttac tgatgaagga
     8581 tgtaacttgt accaggggta ttttgcatta cggtaataat tattacttac agataaccag
     8641 cgatccgtta ttggcaatat tgtttcagta gtgagtagtg ttctgcctga atacggtaac
     8701 ggtaaactgg acgccccgtg acgccataat ggatactggt gaacaagatg tggcagttga
     8761 ccagccagat aaggtattta cggcaggaaa cacgcgaaat gttaacctcg ttggctagct
     8821 cgtcggttga aaactcatag tcctgatgcg cgtcaatcca ctggcacagt gtgcgtaacg
     8881 tctgcggcgt taagcctttt ggcaagcgac gaggatcctg ctcgttggag ctgctgccgt
     8941 ggattagctg atcaagctcg gcctggtcat aatactgatg tttttccagc gccattttct
     9001 tttgccgcca gccggtgagc gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt
     9061 aatccacgac accgtaatgc agcgaatctt taatggttgc cgcatcggct gcggaggaga
     9121 tgacaatcac atcacttttg caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt
     9181 tctctttttg catatagata tcgagcaata tcaggtcgat aggcgtatcg ctattgaaga
     9241 taatctcttt ggctttctcc agcgtcgagg ctgttccaca gcattgaaag cctgggattt
     9301 gtgctacgta tcggcgattc agctccgcga ccattgcgtc gtcatcgata attaatacat
     9361 tgatcatctg ttcgacctct cccggtccca gggtatctgg acaaaaaatt gtgtgaaaat
     9421 cccgggttcc gattccacgg cgatgctgcc gccgagattt tctacctgtt gtttgacaag
     9481 tgctaaaccg acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc
     9541 gattttgtcg ggtgcgatcc ccggtccatc atcgttaact tcgcagtgca gccagccgtg
     9601 acggtagtgc aatgttacgc taatttcgcc accgggttcc ggccctaatg cttccagcgc
     9661 gttttctatc agatttccca atgtggtaat cagcgtcgcg acctggtcct cactgccgct
     9721 gtctggcagc tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt
     9781 aatcttgctg attaaaaaac cagcgataac cggagatttg atcttaccca gcagagagcc
     9841 aatctcttcc tgatagttat tggctgtttt gagaatgtaa tcttccaact gcttataact
     9901 cttcagatgc aataatccga gaatcacatg caatttattc ataaattcgt gggatcgttc
     9961 acgaagtgca tcagcatagt tgaccagacc gtcgagtcgc tgcatcagtt tacgaacttc
    10021 agttttgtcc ctgaaggttg aaatggcacc gatgataacg ccattactgc gcaccggaac
    10081 ggtgttgatc agtaatagcc ggtctttaat cgtaatctct tcgtcgcggc gcggcgtacc
    10141 gtcgcgtaac acttccgaga catctaccac ctgtgaccat gagtggctga gcgtcgacag
    10201 tttctcatcg tcctgcgact tacggtaatt cagcaattct tgtgcggcat cgttgatcag
    10261 cgtgacctcg ccgcgatcgt ccacggcaac gacgccttct ttgatagact gcaacatggc
    10321 ctggcgttgc tcaaacagcg tggagatttc gtagggttcc aggccgaaaa ggattttttt
    10381 cagtacctta accagaatgc aggtgccaat cagtccgacc agcatgccaa ataataccga
    10441 ccagataatg ctccagcgac tgtcattgat ctgttgggtc acgcggctta actcaaggcc
    10501 gatcgccacc acgccaattt gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa
    10561 agcctgcgcc agaaaaccgc gattgatagc gacattttct tcgccattca gcgctttaag
    10621 gatgtcatca cctttaaatg gctgaccaat acgctgggct tcaggatgcg agtagcgaag
    10681 actttgcata tcggtaacga caataaacag cagatcgttg cgtttgcgca cggcttccgc
    10741 gatggcctgg atgccgctct cctgcggttt tttctgcaag ccctgacgga tttccggcga
    10801 gtcggcgaga gtacgcgcca ctgccagtgc cttgttggct agcccgtctc gcgtcatatc
    10861 actgatttgc gagaagtaaa tcagatgcac caccaatagc accgagaaca gtaccgcgct
    10921 gaccattaag atcactgtgg tactcaattt catcggacgt ttgcgtaaca tgcggtaggg
    10981 caatgaatgt ctcatcagct tccttgtgtg acaaatttct taagcattat ctctgatgag
    11041 gcgggtaatt caaagggagt aagaatgatt ggctatatag gggaagagac tctggcaacg
    11101 gaaactgcca gtgctgtatg aagattccag ggctatgctt atagtgaaaa ccatactgat
    11161 gagagaggga aggtcatgga tcaggcgcta ctggacgggg gttatcgctg ttataccggc
    11221 gaaaagatcg atgtctattt caacactgcg atatgtcagc attctggcaa ttgcgtacgt
    11281 ggcaacggca agttatttaa tctcaaacga aagccgtgga tcatgccgga tgaagtcgac
    11341 gttgccactg tggttaaagt gattgatacg tgcccgagtg gtgcgctgaa ataccgtcat
    11401 aaataagcga gggtaaaatg gaaatacgcg aaggccacaa taaattttac attaatgacg
    11461 aacaaggcaa gcaaatcgct gaaattgtct ttgtgccgac cggagagaat ttagcgatta
    11521 tcgaacatac cgatgtcgat gaaagcctga aagggcaagg gattggtaaa cagctggttg
    11581 cgaaagtcgt ggaaaaaatg cgtcgggaaa aacgaaaaat tatcccatta tgtccatttg
    11641 cgaaacacga atttgataaa acgcgggagt atgatgatat tcgcagttga tggaagagta
    11701 cagattcacg atatttttca tgctctccgc ggtgtgatgc aggagagcat ctgaagggta
    11761 gggggatgca caaagaatgg gcagagagcg cgtttttttg tcccaagtca tcccctttac
    11821 tgagcaaaaa aaagaatatc tcctatatga gaatcatcaa tcggggttaa taaattttgc
    11881 gtccccagag tgttcaatat ttataggagt catattatgg aaggtaaaaa caagttcaat
    11941 acttatgttg tttctttt
//
LOCUS       DAZFBR010000127        11846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFBR010000127 DAZFBR010000000
VERSION     DAZFBR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.127"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            327..4310
                     /gene="ehaA"
                     /locus_tag="ACHJZ2_004050"
     CDS             327..4310
                     /gene="ehaA"
                     /locus_tag="ACHJZ2_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO4570070.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4452..5540
                     /locus_tag="ACHJZ2_004051"
     CDS             4452..5540
                     /locus_tag="ACHJZ2_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4570071.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5582..6513)
                     /locus_tag="ACHJZ2_004052"
                     /pseudo
     CDS             complement(5582..6513)
                     /locus_tag="ACHJZ2_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(6605..7112)
                     /locus_tag="ACHJZ2_004053"
                     /pseudo
     CDS             complement(6605..7112)
                     /locus_tag="ACHJZ2_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            7370..7975
                     /locus_tag="ACHJZ2_004054"
     CDS             7370..7975
                     /locus_tag="ACHJZ2_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO4570072.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            8015..8878
                     /locus_tag="ACHJZ2_004055"
     CDS             8015..8878
                     /locus_tag="ACHJZ2_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4570073.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8868..10415
                     /gene="fdrA"
                     /locus_tag="ACHJZ2_004056"
     CDS             8868..10415
                     /gene="fdrA"
                     /locus_tag="ACHJZ2_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4570074.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10415..11833
                     /locus_tag="ACHJZ2_004057"
     CDS             10415..11833
                     /locus_tag="ACHJZ2_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4570075.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
BASE COUNT         3129 a         2741 c         3195 g         2781 t
ORIGIN      
        1 cacttttacc ggctgtatat taaatgtatt tctcctgctt ttgtttatat tttgagttga
       61 tttgagttcc cggatacata taatgcgcag gtattagaga ctggggggga atgtaatgat
      121 aaagttaaga gtgttaactt aaaataagtt tttagtagta acttgctatt ttattaccct
      181 ctgaactaca tactcagata tgtggttgcc aatgctatgc aaaatttttg aatttttata
      241 tgcgatgaac agtaaccgga atggcattta atgcgttgct gtttatgcaa tcttggtttt
      301 atttggatgt attgctggag attgtgatga ataaaatata tcggctaaag tggaacaggt
      361 cccgtaactg ttggagcgtc tgctcggagc tggggagcag agtaaaagga aaaaagtccc
      421 gggctgtttt aattagcgcg ataagtttat attcatctct ggtattcgcc gatgatgtca
      481 tcgtaaacca ggataaaact attgattttg gcaaagagaa ccagagcatc gattaccgta
      541 ttacggtgac agacaatgcc aatctggtaa tcaatgcgac agatacttcc cgtccgcgtc
      601 tgactctcgc ttctggtggt gggttggata ttaccggagg aaaggtaaca atcaatggcc
      661 cacttaactt tttgctgaaa ggtacggggt tcctgaatgt ctccaatgct ggcagcgagt
      721 tatatgctga tgatttgtat gaatcaaact caggcatgag acacgatcgc ggctatttta
      781 atgtctccaa cggcggcaaa atccatgtta agggcaccag ccgtctgacc tatttgcagg
      841 gaaatgtcag tggtgaaggt agccaggtaa attccgaaac cttctttatg ggcgtttacg
      901 gcagttacgg tggtaatcag tacctgtcag ttaataacgg cggtgaagtt aatgccagga
      961 agcaaattag cctgggctat tatgatcaag tctccgatac aacacttgct gtttcggaag
     1021 gtggtaaaat ttctgcacct actattagtt taagcaccaa ctctgagtta gcgttagggg
     1081 cacaggaagg aagcgcagcg aaggcagcag ggattattga tgccgaaaaa attgagtttg
     1141 tgtgggcaaa gacatccgag aagaaaatca ccttaaacca cacggataaa gacgcgacta
     1201 tttccgcgga tattgtcagt ggcagcgagg gcctgggcta tatcaatgcg ctcaatggca
     1261 cgacttactt aaccggtgat aactctgcct ttagtggtaa agtcaaaatt gagcaaaatg
     1321 gcgctttagg gatcacccaa aatataggta cagcagagat caacaaccgc gggaaattac
     1381 acctgaaggc tgacgatagc atgacctttg ccaataagat ctctggcaac ggtacaataa
     1441 gtatcgacag tgggacggtg gagttgaccg gcaataacta tgcattcagc ggatatattg
     1501 atgttgcttc tggtgctgtc gctgttattt ctgaagacaa gaatatcggt cgtgcagagc
     1561 tggatgtcga tggcaaattg caaattaatg ccaacaaaga ttgggtattt gataacgatc
     1621 ttgaaggtag aggcattgtt gaaataaaca tggggaatca cgaattctcc ttcgatgagt
     1681 ttgcttatac agactggttc cagggttcac tggcgttcca gaacacgaca tttaatctgg
     1741 aaaagaatgc tgagtttctg cagaaaggcg ggatcactgc gggtcaggga agcctggtaa
     1801 cagtgggtaa gggcgctcac tccattagca ctttgggatt ctccggcgga accgttgatt
     1861 ttggtgccct gacagcaggt gcacagatga cagaagggac ggtcaacgtt agtaaaacgc
     1921 tggatttgcg cggcgagggt gtgattcagg tttctgacag tgacgttgtc cgctcagtat
     1981 ctcgtgatat tgactctgcg ttatcgctca ctgaagtcga tgatggtaac agcaccatta
     2041 agttggttga tgcgcaaggt gcggaagttc tgggcgatgc gggcaatctg caattgcagg
     2101 ataaaaatgg gcaaatcctc tccagcagcg cccaacgtga tattcagcag aatgggcaaa
     2161 aagcggccgt cggcacttac gactatcgtc tgacgagtgg ggtaaacaat gacggtctgt
     2221 atattggtta cggcctgacc cagcttgatt tacacgctac cgacagcgat gctctggtgc
     2281 tgagctctaa cggtaaaagc gagaatgccg ccgatctcag cgcaaagatt accggcagtg
     2341 gtgacctggc attcagcagc cagaagggtc agaccgtatc gctttctaac aaagacaacg
     2401 actataccgg tgttaccgat ctgcgcagtg ggacgctttt gttgaataac gataacgtgt
     2461 tgggtaatac ccatgaactg cgtctggcgg cagagactga actggacatg aatggtcaca
     2521 gccagaccgt gggcacgctc aatggcagcg ccgattcact gctgagctta aatggcggca
     2581 gtctgacggt taccaacggg ggcacttcaa ccggttcgtt aacggggagc ggagagctga
     2641 atattcaggg cggcacgctg gacatcgcgg gcgataacag caacctgacg gcgaatgtga
     2701 acattgctaa ttcggctaat gtcctggtaa gtcatgcgca gggattgggt agcgcaaacg
     2761 ttgagaacaa cggtaccctg gcgttgaata atagcgctga aaaaagagcg gctgcgtctg
     2821 tgaattacgc cctgggcggc aatctgacca acaacggtac gctgatgacc ggaatgtcag
     2881 gacagcaagc tggcaatgtg ttagtggtga aggggaacta ccacggtaat aacggtcaac
     2941 tagtaatgaa tacggtactg aatggcgatg actcagtaac cgataaattg gttgtcgagg
     3001 gcgatactag cggcacgact gccgttacgg tgaataacgc tggcggtaca ggtgcgaaaa
     3061 cccttaacgg tatcgaactt atccatgtag acggtaagtc tgagggcgaa tttgttcagg
     3121 ctgggcgtat cgttgcgggg gcgtatgact acactctcgc gcgtggacaa ggggcaaata
     3181 gtggtaactg gtatctgacc agcggcagtg attctcctga actgcagccg gagccagacc
     3241 cgatgccgaa tccagagcca aacccgaatc cagagccgaa ccctaacccg acacctacgc
     3301 cgggtccgga tctgaatgtg gataatgacc tgcgaccgga ggcgggtagc tacattgcga
     3361 accttgcagc agcgaatacc atgttcacca cgcgtctgca tgagcgtctg ggtaatacgt
     3421 actataccga catggtgacg ggtgagcaga aacaaaccac tatgtggatg cgccatgaag
     3481 gtggtcataa taaatggcgt gatggcagcg gccagctgaa aacccaaagc aatcgctatg
     3541 ttctgcaact gggaggcgat gtcgcgcagt ggagccaaaa cggcagcgac cgctggcatg
     3601 ttggggtcat ggcgggatat ggcaacagcg acagcaaaac catttcctcg cgaaccggtt
     3661 atcgtgcaaa agcgagtgtg aacggatata gcacaggcct ctatgccacc tggtatgccg
     3721 atgacgagtc gcgtaatggc gcgtatctcg acagttgggc gcagtacagc tggtttgata
     3781 acacagtgaa aggggatgac ttacaaagtg aatcctataa atcaaaagga tttaccgctt
     3841 cactggaagc tggatacaaa cacaaattag ctgaatttaa tggcagccag ggaacgcgta
     3901 atgaatggta tgttcagccg caagcacagg ttacctggat gggagtcaaa gccgataagc
     3961 accgcgaaag caacggaacc ctcgttcata gcaacggtga tggcaatgtt caaacccgac
     4021 ttggcgtaaa aacctggctg aagagccacc ataaaatgga tgacggtaaa tcccgcgagt
     4081 tccagccgtt tgtagaagtg aactggctac ataacagtaa ggatttcagc accagtatgg
     4141 atggcgtgtc tgtcactcag gatggagccc gaaatattgc tgagataaaa accggggtgg
     4201 aaggacagct aaatgccaac ctgaatgtct gggggaatgt gggcgttcag gttgccgata
     4261 ggggatataa tgacacctct gcaatggttg gcattaagtg gcaattctga tctgaataat
     4321 gagtaaaagg agagagagcc gaacgggaga taattcggct ctctgtgtca ttttcttgca
     4381 gccagcttta tccgtcagag tgaagactcg cagatttagc tggcgcacaa gatttaatca
     4441 aggtgtgttc aatggcttcg catgatttga gtgttttttt ggaagagttc ggggcaacgg
     4501 tgaatttgac gttgcctggt atcgtgtccg aaaaagaacg gcttttactc aagctgctaa
     4561 tggagggaat gtcggttaca gaaatctcac agtacagaaa tcgcagtgca aagacaattt
     4621 cacatcaaaa gaagcagctg tatgaaaaac taggcatcca gagtgatatt accttctggc
     4681 gggatatctt ttttcaatat catccgcaag tcattagtgg cacgggaaat aaaaataatt
     4741 tttatatccc tgataaccgt tgtcaccata tcgtcacgcc tgaagccatc agtctggcgt
     4801 tggaaaacca tgaatttaaa ccgtggatcc aaccggtttt ttgcgcgcag actggggtac
     4861 tgacgggctg tgaagtgctt gtccgctggg aacatccaca aacgggaatc atcccgccgg
     4921 atcagtttat tcctctggcg gagtcatccg gtcttattgt cataatgacc cgccaactga
     4981 tgaaacagac tgcggatatt ctgatgccgg taaaacattt gctgccggat aatttccata
     5041 ttggtatcaa cgtctcggcg ggttgttttt tggccgcggg atttgaaaaa gaatgcctga
     5101 acctggtcaa gaaattagga aacgataaaa tcaaactggt tctcgagcta acggaacgta
     5161 accctattcc ggtaacgcca gaagccagag cgatatttga cagccttcat cagcataaca
     5221 ttacctttgc gctggatgac tttggtacgg gttatgcgac ctatcgttat ctgcaggcgt
     5281 ttccggtcga ttttattaag atcgataagt catttgtgca aatggcgggt gtcgacgaaa
     5341 tatccggtca tattgtggac aatattgtcg aactggcgcg taagcctggt ctgagtatcg
     5401 tggcggaagg ggtagaaacc caggagcagg cggatttaat gatcggtaaa ggcgttcact
     5461 ttttgcaggg ctatttgtac tctccgccag taccgggtaa taaatttatc tctgaatggg
     5521 taatgaaagc aggtggttga tgtaaaccgc tattcacagc gcatcgggag gttggcagcg
     5581 attaattctc ccgatgcagt aaatcctgat agatccccgt taatatgcca tttggcgcaa
     5641 attgtttttt aatccactgc gtcacctgac ccgtagcaga atgctgagtc gccagtaaca
     5701 tgcgtgaatc ctggcgcggg ttatggattt gtcgggtaac cagcagggat tgcgccatcg
     5761 cctcacggac catgtaatct ggcaaaaaaa caatcccttc acctaaaatc tggcactgac
     5821 atttggtgtt gaaatctggc accagaattg actcctgccc gtgcagcaac cagcccactt
     5881 ttttattaat cgtatgcgcg gtgtcctcca ccataatatt agggtagaga cgcagttggc
     5941 tttcggcgat ggttccggca caaaggctaa cgggtgatcc ggggcgatag caaaagccca
     6001 gcgaatcgcg ccaatttcag tgtaatcaat accgccgccg tccagcagcg tgtcgggtgc
     6061 gccgatagcg atatttgcct gattattaat aatcgcatcc cagacgccgt tatacacttc
     6121 ggtggtgacg gtaatctggc aggtaggaaa ctgctttttc agcacctgta ataagcgtgc
     6181 ggtgtgtttg ggcgtataaa gcaactgatt aatacaaatg cgcacacgcg cttctatgcc
     6241 ctgcgaaata gtatcgatgc tgcgtttgat ggcgtgaaaa tcattcagca ggtcggtagc
     6301 tttgcggaaa aaataacgcc cggactccgt taactcaatg ctgcgggtac tgcgagtgaa
     6361 cagcaccaca tccagccctg tttccattcg cttgatggtg tagctaatgg cggaagtggt
     6421 taaacccaac tcttctgcgg ctttactgaa actgccaaaa cgcgcagcgg tagtaaatgc
     6481 caacagattc tcttcggtaa aaattgagtt cattaaacca ttcccgccat tatctaatat
     6541 gaatttctcc aagcataact gtagaggtat caattagaga tgggtaatca tttttgaata
     6601 acttttaata cccgtcgcgt ttagtcgtcg cttcgcttaa tttttgtccg ccatattctg
     6661 aaattatccc cacgcagtta cccgccagaa aacccagtaa cgtcaccgtc cagttcatcc
     6721 cgctggcaaa aaacagcgtc atgccgagaa agccaccggg aataaatgac agtaaccaga
     6781 aacgcccttg ccagaccacc accgccgaga agggtacggt agtaattacg ctcgcccaga
     6841 acgcggacag tccacttaca gaggccagcc aaccggcagc cagcgcggcg aaaaacgccc
     6901 acaccatccc ggaataatta accgacagac ttctgataaa ccccgattta ccgccgcctg
     6961 cggcataaaa actgcaaaag gcgacgaagc caatggttcc taataattcc taataattcc
     7021 catccctgag agagcccgac atgtgaagac actaactgcc aaagccctgc acaaataccg
     7081 acggtgacac ccgttgccgt gagtccgttc atcttgttcc cctctggtta gacatggacg
     7141 aaagctactc ttacatgtgc agactataga gaaataaaac ctgattgagt gaaagggtat
     7201 cgggtcaaag aaacaaatat tgcactaccg cacactgcga aaagattgtt gaacatcatt
     7261 caacaaaaag gcgaaatacg gcgaattaaa tttaagcggg ttcttttatc agattatcgt
     7321 tgctattctc aattcatcga aaacagccac aaaaaccagg ggaatgtgaa tgagtataaa
     7381 aaatctacct gccgactatt tattggctgc acaacagggt gatatcgata aagtaaaaac
     7441 ctgccttgcg ctgggtgtcg atattaatac ctgcgatcgt caggggaaaa cggcaattac
     7501 gctggcaagt ttatatcagc aatatgcttg cgttcaggca ttaattgatg ccggagcgga
     7561 tattaataag caagatcata cctgtttaaa tcctttttta attagctgtc tgaacggtga
     7621 tttaacgctg ctacgaatta ttttaccggc taaaccagac cttaattgcg tcacccgttt
     7681 tggcggtgtc ggcctgacgc ctgcctgtga aaaaggccat ttaagtattg taaaagagct
     7741 tttggcgcat acggaaatta acgttaacca gaccaaccat gttggctgga cgccactact
     7801 ggaagcgatt gtgcttaacg atggcgggat taaacagcag gcgattgtgc agttattgct
     7861 ggaacacggt gccagcccgc atctgaccga taaatatggc aaaacgccac tggaactggc
     7921 gcgggaacgg ggctttgaag agattgcgca gttactgatt gccgcaggtg cataaaccgg
     7981 gaggcttgct atcaacacac cagaaaaacg gtgtgtgtgg gcgctaactg cggatgcgga
     8041 ttttctggcg cagcgagggc aaggacaggt tgaacaggtc tttgccagag cggtaaatat
     8101 cgcactcccg gctcgccagc agttgctgac gctgctttgt gaagagtacg acaatgcgcc
     8161 aaacagttgt cggttggcac tcactcactt tgatgatctg ttccggcatg gtgataaggt
     8221 tcagtttgac gatcaaggta ttacggttgg tcaacatctt catatagaga tgagtcgttg
     8281 tcggcgttgg ctgtccccaa ccttgcaaat gaccgctgtg aattttcgcc ttatcgcctg
     8341 gcaacagtgg cacgacatca ttcatcagca cctgggggaa aatgaaaccc tgtttaatta
     8401 tcgcggcgat aatccgtttt atcaggcgtt aaataaagaa ttacatatta aacgacgggc
     8461 agttatacag gcagtaatcg ataaacaaaa tatcgcctca gcggtcgcca gtatgatggg
     8521 cttagggatt ggccttacgc catcagccga tgattattta acaggtctgg cacttatttt
     8581 atttatttcc gggcatccgg cgggaaaata caaagaggaa ttttatctcg gcttgcaacg
     8641 cggcagaaat aataccacgt tattaagtgc cataacgctg gaagccgcat tacaacaacg
     8701 ctgccgggaa aatattcatc gttttattca caacattatt tatgacatcc ctgggaacgc
     8761 aactcaggca atagaaaaaa ttaaacatat tggctccagt tccggctgcg acatgctgta
     8821 tggcatggcc gatggttgtg cgctgagcca aacctacgga gggaattatg tcagttaaaa
     8881 tagtcattaa accgaatacc tattttgatt ctgtctcgct gatgtctatc tccacgcgtg
     8941 caaataaact cgacggcgtc gagcaggcat ttgtggcgat ggcgaccgaa atgaacaaag
     9001 gtgtgctgaa aaatttagga ctgctgacgc cggagctgga gcaggcgaaa aacggcgacc
     9061 tgatgattgt catcaatggt aaatcggggg cggacaacga gcagttgctg gtggagattg
     9121 aagaactgtt caacaccaaa gcgcaaagcg gctcgcacga ggcgcgttac gccactattg
     9181 ccagcgccaa aaagcatatc ccggaaagta acctggcggt gatttcggtc aacggtctgt
     9241 ttgccgctcg cgaagcgcgt caggcgctgc aaaacgatct caacgtgatg ctgttttccg
     9301 ataacgtctc agttgaagat gaactggcgc tcaagcaact ggcccacgaa aaagggctgc
     9361 tgatgatggg gccagactgc ggcacggcga ttatcaacgg tgcggcgctc tgctttggta
     9421 acgccgtgcg tcgcggcaac atcggtattg ttggcgcatc cggcaccggc agtcaggagt
     9481 tgagcgtccg cattcatgaa tttggcggcg gcgtttcgca actgattggc accggcgggc
     9541 gcgacctgag cgagaaaatc ggcggcctga tgatgctcga cgccatcggg atgctggaaa
     9601 acgatccgca aactgaaatc attgcgctta tctccaaacc gcctgcgcct gcggtggccc
     9661 gcaaagtgct ggaacgtgcg cgcgcctgcc gcaagccggt ggtcgtttgc ttcctcggtc
     9721 gtggcgaaac gccagtggat gagcaggggc tacagtttgc ccgcggcacc aaagaggcag
     9781 cgctaaaagc ggtgatgctc tccggcgtga aacaagaaca tctcgacctg cataccctga
     9841 accagccgtt gattactgac gtgcgcgctc gcctgaagcc gcagcagaaa tacattcgtg
     9901 gcctgttctg cggtggcacg ctgtgcgatg aaacgatgtt cgcggtgatg gaaaaacaca
     9961 gcgatgtcta cagcaacatc cagcccgatc cggaattccg cctgaaggat atcaaccgca
    10021 gcatcaaaca caccttcctc gactttggcg atgacgactt caccaacggc aagccgcacc
    10081 cgatgattga ccctaccaac cgcatcagcc gcttgatcga agaggcgcgc gatccagaag
    10141 tggcggtgat cgtgatggat tttgtgctcg gatttggatc gcatgaagat ccggtcggtt
    10201 ccaccatcga ggcgatcaaa gaagcgaaag cgatcgccgc tgccgaagga cgcgagttga
    10261 tcattctcgc ctatgtgctg ggtaccgatc ttgatacgcc atcgttagaa caacaaagcc
    10321 agatgctgct tgatgccggc gtgattctgg cgagcagcag caccaatacc ggattgctgg
    10381 cgcgtgaatt tatctgcaaa ggggaggaag cctgatgagc cagtcactgt ttagccaacc
    10441 attgaacgtt attaacgtcg gcatcgccat gtttagcgat gacctgaaaa agcagcatgt
    10501 agaagtgact caattcgact ggacgccgcc ggggcagggc aatatgcagg tggtgcaggc
    10561 gctggataac attgccgatt cgccgctggc agacaaaatc gccgccgcta accagcaggc
    10621 gctggagcgc attatccagt cgcatccggt gctgattggt tttgatcagg cgattaacgt
    10681 ggtgccgggc atgacgccga aaaccattct tcacgccggg ccgccgatca cctgggaaaa
    10741 aatgtgcggc gcgatgaaag gcgcggtcac cggagcgctg gtgttcgaag gactggcgaa
    10801 agatctcgac gaggcggctg aactggcggc ttccggggag atcaccttct cgccgtgtca
    10861 cgagcacgac tgcgtgggat cgatggcggg tgtgacctcg gcctcgatgt ttatgcacat
    10921 cgtaaaaaac aaaacctacg gcaacatcgc ttataccaac atgagcgagc agatggcgaa
    10981 gattttgcgt atgggcgcta acgaccagag cgtgattgac cgcctgaact ggatgcgtga
    11041 tgtgcaggga ccaatactgc gcgacgcgat gaaaattatc ggcgaaatcg atctgcgctt
    11101 aatgctggcg caggcgctgc atatgggcga tgagtgccat aaccgcaata acgccgggac
    11161 gacgctgctg attcaggcgc tgacgccggg gattatccag gcgggctatt ccgtcgcgca
    11221 acagcgcgaa gtgtttgagt ttgttgccag tagcgattac ttctccggcc cgacgtggat
    11281 ggcgatgtgt aaagcggcga tggatgcggc gcacggcatc gaatacagca ccgtcgttac
    11341 caccatggcg cgtaacggcg tcgagttcgg cctgcgggtg agcggcctgc cggggcagtg
    11401 gtttaccggc ccagcgcagc aggtcatcgg cccgatgttt gctggttata agccggaaga
    11461 ttcggggctg gatatcggcg acagcgccat cacagaaacc tacggtattg gcgggtttgc
    11521 catggcgacc gcgcctgcaa tcgtcgcgct ggtggggggc acggtggaag aggctatcga
    11581 tttctcccgt cagatgcgcg aaatcaccct tggcgaaaac cccaacgtca ccattccgct
    11641 gctcggtttt atgggcgtgc cgtcggcagt cgacatcacc cgcgtgggta gcagcggcat
    11701 tctgccggtg atcaacaccg ccatcgccca taaagatgcg ggcgtcggca tgattggcgc
    11761 gggcattgtg catccaccat tcgcctgctt cgagaaagcc attcttggct ggtgcgaacg
    11821 ttacggcgtt tgacctacct gttcat
//
LOCUS       DAZFBR010000128        11740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFBR010000128 DAZFBR010000000
VERSION     DAZFBR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.128"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..843
                     /locus_tag="ACHJZ2_004058"
     CDS             <1..843
                     /locus_tag="ACHJZ2_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO4570076.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAM
                     ARIK"
     gene            979..1662
                     /locus_tag="ACHJZ2_004059"
     CDS             979..1662
                     /locus_tag="ACHJZ2_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570077.1"
                     /translation="MKLPVKLLMSLISLVRVIARTGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            1678..2088
                     /locus_tag="ACHJZ2_004060"
     CDS             1678..2088
                     /locus_tag="ACHJZ2_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570078.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            2309..3130
                     /locus_tag="ACHJZ2_004061"
     CDS             2309..3130
                     /locus_tag="ACHJZ2_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4570079.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            3212..3691
                     /locus_tag="ACHJZ2_004062"
     CDS             3212..3691
                     /locus_tag="ACHJZ2_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4570080.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSAIMRIID"
     gene            3706..4182
                     /gene="yeeS"
                     /locus_tag="ACHJZ2_004063"
     CDS             3706..4182
                     /gene="yeeS"
                     /locus_tag="ACHJZ2_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO4570081.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRGPDHLIVGGNQVFSFAEHGLL"
     gene            4245..4466
                     /gene="yeeT"
                     /locus_tag="ACHJZ2_004064"
     CDS             4245..4466
                     /gene="yeeT"
                     /locus_tag="ACHJZ2_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4570082.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            4540..4908
                     /gene="cbeA"
                     /locus_tag="ACHJZ2_004065"
     CDS             4540..4908
                     /gene="cbeA"
                     /locus_tag="ACHJZ2_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO4570083.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            4997..5370
                     /gene="cbtA"
                     /locus_tag="ACHJZ2_004066"
                     /pseudo
     CDS             4997..5370
                     /gene="cbtA"
                     /locus_tag="ACHJZ2_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            5367..5561
                     /gene="yeeW"
                     /locus_tag="ACHJZ2_004067"
     CDS             5367..5561
                     /gene="yeeW"
                     /locus_tag="ACHJZ2_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4570084.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            5607..5687
                     /locus_tag="ACHJZ2_004068"
                     /pseudo
     CDS             5607..5687
                     /locus_tag="ACHJZ2_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5976..6122)
                     /locus_tag="ACHJZ2_004069"
                     /pseudo
     CDS             complement(5976..6122)
                     /locus_tag="ACHJZ2_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            6176..6358
                     /locus_tag="ACHJZ2_004070"
     CDS             6176..6358
                     /locus_tag="ACHJZ2_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO4570085.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(6459..6788)
                     /gene="tmaR"
                     /locus_tag="ACHJZ2_004071"
     CDS             complement(6459..6788)
                     /gene="tmaR"
                     /locus_tag="ACHJZ2_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO4570086.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(6960..8018)
                     /gene="yeeA"
                     /locus_tag="ACHJZ2_004072"
     CDS             complement(6960..8018)
                     /gene="yeeA"
                     /locus_tag="ACHJZ2_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO4570087.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(8217..8690)
                     /gene="sbmC"
                     /locus_tag="ACHJZ2_004073"
     CDS             complement(8217..8690)
                     /gene="sbmC"
                     /locus_tag="ACHJZ2_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO4570088.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(8809..9975)
                     /gene="dacD"
                     /locus_tag="ACHJZ2_004074"
     CDS             complement(8809..9975)
                     /gene="dacD"
                     /locus_tag="ACHJZ2_004074"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO4570089.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            complement(10814..10971)
                     /locus_tag="ACHJZ2_004075"
                     /pseudo
     CDS             complement(10814..10971)
                     /locus_tag="ACHJZ2_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            11299..>11740
                     /gene="nleG7"
                     /locus_tag="ACHJZ2_004076"
     CDS             11299..>11740
                     /gene="nleG7"
                     /locus_tag="ACHJZ2_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO4570090.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGV"
BASE COUNT         3115 a         2937 c         2738 g         2950 t
ORIGIN      
        1 ctgtcggtaa acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac
       61 ctctgccaga tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg
      121 acaatcaacg ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca
      181 gcgctgatgg atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg
      241 tcgctgtacc ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg
      301 ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag
      361 cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt
      421 caggggacaa tgttcggtga tggtggtatg ggaggatggc gaaatatgca gggaaatacc
      481 cctgtgacgc ctctggtcga tgccggatgc aatatggtga ttgtgacgca tctgagtgac
      541 ggttctttat gggatcgcca ggcttttccg gacaccacaa tccttgagat ccgtccccgg
      601 aaaaggctga aatatgcagg tgatggtggc aacagcggcg gtctgctcag ttttacatcg
      661 gcacataccg acgcctggcg tcagcagggc tatgaagaca cgatgctggc gatggagcat
      721 atccggaaac cgctggcagc acgtcaggca ctgacccggt cagaggcggt attgcagaaa
      781 agcctggata taacggaaga ggcagatttg gcactgagaa acgcgatggc ccggattaaa
      841 taaagacgcc ccaaagaaac gccaccgcct gtggtggtgt ttttcctgtc tggtgttatt
      901 gaaaatattt tcagatatat attacctgag agaaagtttt acccctcacc tgtcaattca
      961 ctcactggaa atttaattat gaaattacct gtaaagttat taatgagcct tatatctctg
     1021 gtccgcgtta ttgcacgtac cggggaatat aaaaattaca gccgggatga aataaaatac
     1081 tggcgatata catcatacaa gggggggaaa cttccggaag gtttcactga tgagaaattt
     1141 tccagcgcca tttacaacgg aagaatattt acaatgaaac gtttacatac ccttatgtta
     1201 tttctggcgg ttctgttcac gggctttaac tttaacgcag aagcggctac cgtaaaacaa
     1261 gctcttagtt gtaacccgga agcctgggct gaacaacctg gagcgtgtcc atcaacgtac
     1321 gagttgtacg aaggtgacgc tacctacaaa gccgctatcg acaaagcatt aaaaccggtc
     1381 ggactgagcg gcatgttcgg taaaggcggc tatatggatg gccctggtgg aggtatcacg
     1441 ccagtaaaca ttaacggtac agtctggttc cagggcgacg gttgcaaagc caatacctgc
     1501 ggctgggact ttatcgtaac actctataac ccaaaaaccc atgaagttgt tggctaccgt
     1561 tactttggtt tagatgaccc ggcctacctg gtttggttcg gtgaaattgg cgtgcatgaa
     1621 ttcgcgtatc tggtgaaaaa ctacgtagct gcggttaact aatttaagga gtctatcatg
     1681 aaaactttta tcaaaacttt actcgttgct gtaactattc tgttctctgt cttcgctact
     1741 gcgaaacaag taaaactgcc aaataacatc aaatacgtta atactacaga agcgttttcc
     1801 tgtaccgaaa ttgacggtat gaattgccag acaaagaatc agttcaacta taaagataac
     1861 agctatgttt tcgtgcttga acgtggtggt gcctggtgct acgactacac tgtctcggta
     1921 gttaacctga aaaccgggaa agcacaaatg attgaatacg gggacaatca actgtgctca
     1981 ggtagcaaca aaccgttctt cgaaatcaaa aatggcgtac cgacggtagg agtcatcgac
     2041 acatccggaa aacctgtcgg tgtagcccag gacaaactta aaatctaagg agtaaaccat
     2101 gcgtttctct gcacattatt aatatcaaag gtaaatacag ccccagccat tgttaccgga
     2161 aagccgctta atgcgtggct cgccagtgaa aggcctctca gacaataaac cttattcatt
     2221 tcacccgtca ggccgcgtct tctccgggag acgcggcttt tttcatttat tgcaccagta
     2281 aatcttaacc accgataagg agcaaagtat gcgattagcc agtcgttttg gttatgctgc
     2341 aaaccagata cgccgtgacc gtccgctgac acatgaagaa ctgatacgcc atgtacccag
     2401 tatttttggg gaagaccggc acacctcccg cagtgaacgg tatgcgtaca ttcccaccat
     2461 taccgtcctg gaaaatctgc agcgggaagg ctttcagccg ttcttcgcct gccagacccg
     2521 tgtgcgcgac ccgggccgcc ggggatacac aaaacacatg ctgcgtctgc ggcgggccgg
     2581 agagataaac ggagaacatg tccctgaaat tattctgctc aactctcatg acggtacctc
     2641 cagctaccag atgctgccgg gttacttcag gttcgtctgc cagaacgggt gcgtctgtgg
     2701 ccagtctctg ggggaagtgc gtgttccaca ccggggaaat gtagtggaga aagttatcga
     2761 aggggcttac gaggtggtgg gcgtgtttga ccggatagag gagaagcgtg atgccatgca
     2821 gtcgctggtc ctgccgcaac cggcacgcca ggcgctggca caggcggcac tgacttaccg
     2881 ttatggtgac gaacatcagc ccgtcaccac cgccgacatt ctgacgccgc gacgccggga
     2941 ggattacggt aaggacctgt ggagcgcata tcagaccatc caggagaata tgctgaaagg
     3001 cgggatttcc ggtcgcagtg caaaaggaaa acgtatccat acccgggcca ttcacagcat
     3061 tgataccgac attaagctca atcgcgcatt gtgggtgatg gcagaaacga tgctggagag
     3121 cctgcgctga tgccgtttcc ctgagtgagg gaatcattca gctttcgttc ccgaaaaaca
     3181 cacatttatt tgttatttaa ggagttatct catgaaaacc gtttctcaga atacccccac
     3241 aatttattca gctacaacac cagagaataa tccgcctcag ttggttgcca gcctcgtccc
     3301 tgatgaacag cgcatcagct tctggccgca gcattttggc ctcattccac agtgggtcac
     3361 cctggagccc cgtgtcttcg gctggatgga ccgtctgtgc gaagactact gcggtggtat
     3421 ctggaatctg tacaccctga acaacggcgg ggcatttatg gcccccgaac cggatgatga
     3481 tgatgacgaa acatgggtac tgttcaatgc catgaacggt aaccgcgctg aaatgagccc
     3541 ggaagccgcc ggtattgccg cctgtctgat gacgtacagc catcatgcct gtcgtacgga
     3601 gaattatgcc atgacggtcc attattaccg gttgcgggat tacgccctgc agcatccgga
     3661 aggcagcgcc attatgcgca tcattgactg aaaggggccg gaataatgca acagatttcc
     3721 tttctgcccg gagaaatgac gcccggcgag cgcagcctca ttcaacgggc cctgaaaacc
     3781 ctggaccgcc atcttcatga acccggtgtg gccttcacct ccacccgtgc agcacgggaa
     3841 tggctgattc tgaacatggc gggactggag cgtgaagagt tccgggtgct gtatctgaac
     3901 aaccagaatc agctgattgc cggtgaaacc ctcttcaccg gcaccatcaa ccgcacggaa
     3961 gtccatcccc gggaagtgat taaacgcgcc ctgtaccaca atgccgctgc cgtggtactg
     4021 gcacacaatc acccgtccgg tgaagtcaca cccagtaagg cagaccggct tatcaccgaa
     4081 cgtctggtac aggcactggg cctggtggat atccgggggc cggaccatct gatagtcggt
     4141 ggcaaccagg ttttctcctt tgccgaacat ggtctgcttt aacccgtcac aaccacatca
     4201 cacctgtttt cacttttatc ttctgtcttc agaggtatcc cattatgaaa attatcaccc
     4261 gtggtgaagc catgcgtatt caccaacaac atccgacatc ccgtcttttt ccgttctgta
     4321 ccggtaaata ccgctggcac ggcagtgctg aagcgtatac cggtcgtgaa gtgcaggata
     4381 ttcccggtgt gctggccgtg tttgctgaac gccgtaagga cagttttggt ccgtatgtcc
     4441 ggctgatgag cgtcaccctg aactgagtgg gaattctgat gagcagaatt atcgccacta
     4501 ccggaccatt cttagccgat tttctgtaag gattttatcg tgtcagacac actccccggg
     4561 acaacacttc ccgacgacaa tcacgaccgc ccctggtggg ggctgccctg caccgtgacg
     4621 ccctgtttcg gggcacgtct ggtgcaggag ggtaaccggt tgcattacct tgccgaccgc
     4681 gccggtatca gaggcctgtt cagcgatgca gatgcgtacc acccggacca ggcctttccg
     4741 ctgctgatga aacaactgga actcatgctc accagcggtg aactgaatcc ccgccatcag
     4801 cataccgtca cgctgtatgc aaaagggctg acctgcaaag ccgataccct cagcagttgt
     4861 ggttacgttt atctggctgt ttatccgacg cccgaaatga aaaattaact ctccagaata
     4921 gccttctgct acggcctggt gttttcacca cgccactttt ccatttttat atctgcatat
     4981 caggaaaatc ttcagtatga aaacattacc tgtattaccc gggcaggcgg ccagttctcg
     5041 cccgtctcct gttgaaatct ggcagatact gctgtcccga ctgctggacc agcactatgg
     5101 cctcacactg aatgacacac cttttgccga tgaacgtgtg attgagcagc atattgaggc
     5161 aggcatttca ctgtgtgatg cggtgaactt tctcgtggaa aaatacgcgc tggtgctacc
     5221 gaccagccgg gattcagcgc ctgtacccgc tctcagttaa taaacagcat cgatatcctc
     5281 cgggctcgca gggcgaccgg cctgatgacc cgcgacaatt acagaacggt aaataacatt
     5341 accctgggta agtatccgga ggcgaaatga tgacactgga agccgacagc gttaacgtac
     5401 aggcgctgga tatggggcac attgtcgttg acattgatgg tgttaatatc actgaactga
     5461 ttaataaggc cgctgaaaac ggttattcac tccgcgtggt ggatgaccgt gactctaccg
     5521 aaacaccggc aacttatgcc agccctcacc agttgctgta agacaatgca gtgatgcagg
     5581 gtatagtgcc attaaccaca gaaacagcac tgttgtcctg tattaagcag gcaagagaag
     5641 atgcccttac cctgcgccat ctgcagcttc tgcaccagaa ccggtgaatc tcactcaaca
     5701 ggcttcattt cccctgacgc cagcctgaat acagctggcg ttttcattta taaacagaaa
     5761 ggaaaaccga taataatgga atggacgccc cgaccatgaa gcaacggtag gatattactg
     5821 ataaaccttc cagtggaggt gcgttatggc cgttttaacg attagcattg acctgacaaa
     5881 aaatgaattc caaatccacg gtctgggtag gaacagaaaa atctaagctc agaaaacgga
     5941 ttaaagtaag cgtcaacgga gcaccgtatt gacgcttatt tattggtgag aactacgttc
     6001 catggcagga gttcgtcaac acggttggag ggccattccg gcagtatgct cagaatatgg
     6061 cgcagatacg cttccggatc gataccgttc agacggcagg tgcctgcttt tccggcaaca
     6121 agcaggaatc tcagcgctga agcaggaatt agaggtaaaa acgccctacc gggccatgaa
     6181 tcacccggtc attggagtag taaccaaagc agatttagcc agcatggaac aaatatcttt
     6241 ggtgaaatgt tggttacggg aagctggagc gcacaacgtg ttagtaacca gtgcggttaa
     6301 taacaatgga gttacagaac tttttgcctt gctgcataca gaagaaggct gccgctaatt
     6361 tcgataggtt ctcagatgag aatgatgacg gtgatacaca gataagaatc aatgagcaat
     6421 gagggttgcc gggcaaccct cattgaataa aacaggaatt acttcgcttc gccgtttttc
     6481 atttcgccca tagctttcag ctttttggag atatcgcggc gttctttgga gagctcggca
     6541 tttttgatga tgtaatcgtc aacgcgatct tcatagtcac ctttcatgct ggcgatgatg
     6601 ccctggattg cttcaacgct catccctggc ttgatgtaat cgctcaggtt gtccagcaac
     6661 aggacgcgct tctggttgtc acggatcttt ttctcaacgt cctgaatttc acgttgcagt
     6721 ttgttcttac gacggaacag acgaacaaat tccagtacgt cctggaatga aggcttggta
     6781 gtttccattt ttatacccct gataatgtga gagtcggatt cgtttaagca accgctattc
     6841 gttagggcca acattactca tagctgccgc agatgacaat gcttttatcc cttatcacta
     6901 tcataccctt tatccttgct gaatcgaagc agcagcaaga tgattctgaa gttcaggaat
     6961 tatttgcgca aggcccggca aatcagactc gatagcaact caagctgatg tgccgtttcc
     7021 atgttcagcc acacataacc atagattggt gtttccacaa ccttcaggtc atggtggtta
     7081 ttcagcaact gacgcagctc ttccacagca tcgttcaatt tttccgtatt ggcaaaaacc
     7141 ggctgcggat taccttcgta cagcgcatga acaaggctca gcagtatttg ctgcatcatg
     7201 tgctgggtat cacgaagttt ttgcgcgttc aataacacga aatggctggg gcgcgtggcc
     7261 cagtatgcat tgatttgcaa ctccagcata caaaccagat tgcggttaat ggtctggata
     7321 ccttcatata tcgattttgg aatacgagtt tctttgctgg cgggcgcaat cagcccacgc
     7381 attttcacgg catcggtcag gagtttttgt agatggcttt ccagacgtgg gcgttcgagt
     7441 aagttcggtg agaatgcaga ttgatagacc cgattatact cggtcagact tttcgccagt
     7501 tgaatgcgcc agtggatgaa cgcccgttgt ggccagatac cggtaaacaa cattgccagt
     7561 aaagagccga ggatcacatc gccgcttcgc cataacgccg tatcaatttc acctgtcggg
     7621 gaacccacaa caattgccag cgtcacccca atcaataatc cttgatacgg tttcttgccc
     7681 agcgccagcc aaccgcaaag aaacatcgcc gccgcgcacc agactaacat cagcggtaac
     7741 gaaattaact ccagttgcag agcgataaga cctaaaatcg accccaacac cgtaccgcca
     7801 atacgttcga aggcacgagg gacaacgttc ccccagaacg agattggccc cataatcacc
     7861 accatggtga ccagcggcca ggtgccttcc gggatagtaa acaggcggat aatgagaaaa
     7921 gtgagcagga atgccagcgc gacccgagta ccatgcacaa tgcggtaatg gcggtatacc
     7981 cggatttcaa acgggcttaa tgacttattg gcacgcacgc agaaactccg ttaatgaaga
     8041 ttaaaagcgc tcataacaca tctgtcggtc agaaggcgcg cctctgaatt atccgacgga
     8101 caaaccggga acagtgtaat ttcccggcag gagcgaaaaa attgaaaggc gcatcttact
     8161 cttttcgctt tcaaaaaaag agtggtcttc gcgtcaacac accgccctga gatgaattag
     8221 tgatgttttg gctgcaccgc aacatacatt tcgatatccc agtacccatc ttccgcgcca
     8281 ttgttcaagt aaacctcgaa gcagggcttt ggtaacattt cataagcact gtcctgcaag
     8341 aggctattaa agaactgata ccagggttta gcaaaatcat caccgactac acgagccacc
     8401 gccaccgcat attgaccacc tgaaatttct gtcagaatga cgccctcact gttttcgggg
     8461 agcgtaaagt tattcggtac cgtcacgacg gtgtcgcagc gtaatttttc ggcgggtgtt
     8521 tcatccggat tgtcgtaata gacagcaacc cactccttcg gcacaatatt tttgctatct
     8581 acccacatca tcaactgctc aaaacctttc tttaccgtct gttcccacgg gccaacgaga
     8641 tggaaacctg caacggtacg tttatcttcc tgcttaatct cgtagttcat gacgcctcca
     8701 ttattactgt ttttatatac agtatagttg caatattaaa accacaagga atgagtgttg
     8761 attatgcgag cagactcgca ctcctgccag tctgctgcaa aagaaaggtc aggccttatg
     8821 gtggaaataa tcactcaggc gagaaaacat gctgccttcc ccgacagatt ccagggtaac
     8881 cagcggccag tgcgccacct gtttatcacg gtcgtaaagt tcaatttccc ctacccgctg
     8941 atgggcgcta attggcgcgg tgagctcttt accatcaagg gtatatttgg ctttgatatg
     9001 tggaatttcg gctttcggta gcaccatcca gaactcttgt tccgttccca gggcgatatt
     9061 ttctttatcg ccataccaga tgcgttccgt accgaccttt ttcccacggt gcaaaatttg
     9121 caccgtagta aagttttgtt gcccccaacg cagtaatttt cttgcctctt cctcacgacc
     9181 ttttgcactg tcagccccca ttaccactgc aatgagacga cgctgcccat ctacagccga
     9241 agcaatgaga ttaaacccgg caccagaagt atgacccgtt ttcaggccgt caacattcat
     9301 ggttttatcc cacaataacc cgttacggtt ttgctgggtg ataccgttcc aggtgagact
     9361 tttctcactg tacatatgat aaaactcggg ctcgccgtgg atgatagcgc gagaaagcac
     9421 agctaaatca taagccgagc tatgctgacc tggtgcatcc agaccatgca ctgtttcaaa
     9481 atgcgtatcc ttgagatgca gcttctcggc atagttgttc atcatttcaa caaactgccg
     9541 ttgcccaccg gcaatatagt cagccagtgc aacacaagcg tcatttccgg aatccacaat
     9601 taaaccacgg cttaaatcac gtaccgatac gcgatcgccc tctttcaaaa acatcagtga
     9661 agaaccgaca aacactggat tatctttcgc ccacgcatcg cgccccacgg tgacaatatc
     9721 gtctggcgta atgcgatgac tatcgatagc gcgatccacg acatagcccg tcatcagctt
     9781 tgtcaggctg gcgggattgc gctgttgatg ctcattaccc gcggtgagga tctgaccggt
     9841 ggtgtaatcc atcaataccc aggagccggc atgaatctct ggaggctgag gtgaaaaagg
     9901 aatgttttcc gccgcaaaac cagacgataa gttaaaaatg aacaaagaag cagcaataat
     9961 aagacggcgt ttcaacagca aaccctcagg agtttcaaat agctgttctt tttacggaaa
    10021 tacttatgaa ctggctggaa taaagtgcaa gaaaatgtga ctaccctctc atttttatct
    10081 gacatgatct gttgccactc gctgtcaaat tgtcgcgcta aagctgatta gcacggtgat
    10141 atttgatact ctggcagaca gcagaaacaa cggatttaac ctaatgatga atgacggtaa
    10201 gcaacaatct acctttttgt ttcacgatta cgcgcaacac ccctgagaac cgccatgaac
    10261 aagtgtttca tgatttgtga ttttagtttg gtacgcaatt tggtacacaa cacaattttc
    10321 acttcagtgg gtagtcatca aactcaccat aacgagcatc gttgatgatg tatgttataa
    10381 ccacaaacat cacatctgga ctagttccgt tttcatgagc gggtatttgt ttacgcctcc
    10441 ctttctgcaa atcctaaaga tgagattttg gggttttggg atatctttta tcctcagttc
    10501 tctatccatt atgcaaacca gtaatgaacc atcaccgtgt gatagtgagg cgtctgccac
    10561 gataagcgca gagaaatgat gcaatggtgg gcggactggc ttgatgaaaa ggtagagtga
    10621 gcgaccttaa actatcgaat agaacaaagt cttgcaatcc agtgcaaagc tttgtgcgcc
    10681 tcagttttgt ctaagcgttc tactgaaaac atagtaaaat cggtaacggc tggaaatcat
    10741 tcaatactcg cactatcgaa agttcgccag ccagccgtgg cacgttcttg catacgacgt
    10801 gccgcggatc ctattattct agtatgaaat ccctaaccga tttcaaattc tccagactgc
    10861 tcagattctc ttttatccca cgctggcgct gataaatctc gtcattgcaa tcgacctgcg
    10921 ctatcgctgc cgcagttctt ccagtttctg catcgataac tccatcttct gagcaataaa
    10981 cctcacctat gcaatatttt atgtcaatta tttcaattgc attaatatct tgaatggatt
    11041 acatagagtt aatgtatcca gtactcccta ttctcttgca tataatacat gttgcaactt
    11101 acatctcagc gctatgaaaa aacaccacct ctctctttat gaaattctgg atttaccaag
    11161 cgctaattta tcatttcaat ctacctttaa gcattgcatt tatctcccta cgaggtcata
    11221 ctttaggaag ttaaatatga atgataatat acctacagcg cgaaatcaca aacaatcgac
    11281 ttgtattaca gaaaaaacat gcctatcttt ttaaacttca cagcaggcag tatcttacct
    11341 gagaatgagc tagcatcttt acgttatatt gtgcagcaaa atcaaaatga tactgtaatc
    11401 ataaaagaac gttataaaat ggatatccgt tatatcgaat cagtcaatgg ttttacagta
    11461 aatcctgtat gcagtaatca tttctccata tttatggcga gacaaaacac tattgctcgc
    11521 aacctggaac agcagatcaa caacggacga agttttgcac aaatatctca ggattttatg
    11581 cttcaattat cttcaaatat aggatggaaa aaaggggccg aaaacgccct taaaaataaa
    11641 atccattctc attcatttgt tgtaaatcct gatgaattct cttgcgacac acaatttctt
    11701 aagtgcccaa taacattatg cgttccagaa aaaggggttt
//
LOCUS       DAZFBR010000129        11636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFBR010000129 DAZFBR010000000
VERSION     DAZFBR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.129"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            61..1035
                     /locus_tag="ACHJZ2_004077"
     CDS             61..1035
                     /locus_tag="ACHJZ2_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4570091.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTLFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(1032..2168)
                     /locus_tag="ACHJZ2_004078"
     CDS             complement(1032..2168)
                     /locus_tag="ACHJZ2_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4570092.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(2174..2497)
                     /locus_tag="ACHJZ2_004079"
     CDS             complement(2174..2497)
                     /locus_tag="ACHJZ2_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO4570093.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2680..2823
                     /locus_tag="ACHJZ2_004080"
     CDS             2680..2823
                     /locus_tag="ACHJZ2_004080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570094.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCPLTIE
                     TRD"
     gene            complement(3042..4580)
                     /gene="aldB"
                     /locus_tag="ACHJZ2_004081"
     CDS             complement(3042..4580)
                     /gene="aldB"
                     /locus_tag="ACHJZ2_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO4570095.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4688..5983)
                     /locus_tag="ACHJZ2_004082"
     CDS             complement(4688..5983)
                     /locus_tag="ACHJZ2_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO4570096.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(6113..7264)
                     /gene="yiaY"
                     /locus_tag="ACHJZ2_004083"
     CDS             complement(6113..7264)
                     /gene="yiaY"
                     /locus_tag="ACHJZ2_004083"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO4570097.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(7453..9297)
                     /gene="selB"
                     /locus_tag="ACHJZ2_004084"
     CDS             complement(7453..9297)
                     /gene="selB"
                     /locus_tag="ACHJZ2_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO4570098.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(9294..10685)
                     /gene="selA"
                     /locus_tag="ACHJZ2_004085"
     CDS             complement(9294..10685)
                     /gene="selA"
                     /locus_tag="ACHJZ2_004085"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO4570099.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(10783..11391)
                     /locus_tag="ACHJZ2_004086"
     CDS             complement(10783..11391)
                     /locus_tag="ACHJZ2_004086"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO4570100.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
BASE COUNT         2853 a         3119 c         2819 g         2845 t
ORIGIN      
        1 cggtgcaaga ttcatattta tatagtaata tataaaatta tatataattg ggctgttgcg
       61 atgacaaaat tacaacttaa gtaccgggag ttaaaaatta tctcggtaat cgctgccagt
      121 gaaaatatca gccatgccgc gactgtactt ggcatcgcac aggccaacgt cagcaaatat
      181 cttgctgatt ttgaatcaaa agtgggttta aaagtctttg accgaaccac ccgacaactg
      241 atgctcacac tttttggcac cgcgctatta ccgtacatca acgacatgct ggacagaaac
      301 gagcagctta ataattttat tgcagattat aagcatgaga aacgtggtcg ggtcaccatc
      361 tacgccccaa ccggcataat tacctattta tccaaacatg taatcgataa aattaaagat
      421 atcggtgata tcaccttatc gctaaaaacc tgcaatcttg agcgtaatgc cttttatgaa
      481 ggcgtcgaat ttcctgatga ttgtgatgtt ttaatcagct atgccccacc aaaagatgaa
      541 tcgctggttg ccagttttat tacccaatat gccgtaaccg cttatgccag ccagcgctat
      601 cttgagaaac atcctattag ccgtcctgac gaactcgaac atcactcctg tattttgatc
      661 gactcgatga tgatcgacga cgcgaatatc tggcgcttca acgtcgccgg gagtaaagag
      721 gtgcgggatt atcgcgtgaa gggaaattac gtttgcgata acacgcaatc ggcgctggag
      781 ctggcacgaa atcacctggg gattgtgttt gcgccagaca aaagtgtgca gagcgatctg
      841 caagacggca cgctggtgcc ctgctttcag cacccgtatg aatggtggct ggatctggtg
      901 gctatcttcc gtaaacggga ataccagccc tggcgggtcc agtatgtgct ggatgaaatg
      961 ctgcgtgaaa ttcgccacca gcttgctcag tcgcagcaac tgcggcctga acaagcggcg
     1021 gaaagcgaag attaatgatc gagataaagg taatgcaccc agctggtcat gcgtaataac
     1081 actttgcgca tcaccgagac atggctgaaa tgatcagagt agaccgccac ctgagcgtaa
     1141 ataccgtctg gtaaagcgct caaatcagtg tgctcattca gttcaatggt cgcgataacg
     1201 ccatctgaac ccggcgctgt gtttaacgtc tgtaaggtgc cggtcgactg ataagctccg
     1261 ccgggaacag ctggactaat ggctgccagc ttaccgctga acactttgcc tggcagagcg
     1321 ttaaatacca cttccgcatc atcgccggga gccaggcgca gcaaggagtt ctgacggaac
     1381 tgcgccacga tttgtcgttt ctgatcgggt ataaacacca tcaccggacg tagcggcagc
     1441 gacgcggcat aggtacccgg acgaatcagc acttgggtca cataaccatc gctcggcgca
     1501 cgcactatcg tctgctcaag gttatatttt gcttccgcga gctgcgcttt caggctggcg
     1561 atttgagaat gttcacccaa caccaggcta tccagctggc tctggatctg tttttgttcc
     1621 gccgccgatg actttacgga ggcttcctgc gccagataat tttgccgtgc cacatcaata
     1681 tcgcgttctg aaaacgggtt tactttcgcc tggctgccgc gtgcgtaacg ctggtactcc
     1741 ttagcgaatt tatcccgcgt ggcctttgcc ttctgagtat tcgccgccat ctcatctaac
     1801 tctgcgccca acgcccgctg tttatgttct gcggtaacga tatccgccat cagccgatcc
     1861 acccgcgcct gataacgcgt cgggtccagt cgaaatagca cctcaccttt tttaatcagc
     1921 gtatttttct tatccgtcac ttctatcacc acgcctgtca cctgtgggac aacaggaata
     1981 gaaataaccg ctttttgcgc tttaaaggta tacggatggt tatagttcat taacagaatt
     2041 agaccgctga caataaatat tccacccagg gccgctgtgg gaatggtcca tttatttacg
     2101 ggaattttga agatcttaaa cattgcccat gcaaaagcca cataggtcaa aataatcagt
     2161 aaatccataa gtattactcg gcagaagagg atttgatgtc ggcgagctgt ttctccagcc
     2221 cggcaatgcg ctgttgcagt tgcataacgg atgaatcatg gctttgcatt ccccatcccc
     2281 gctccgggcg gtaaagcgtg gcccaaatcc acaaaaatgg ccagataaca tgcagcgtaa
     2341 aaagactcac ccaaccagca acatgaatgg cgtcggcatg aggatgatta cgttttttag
     2401 caatcaggta gggaatatca tgtaaaataa taatcccata gaatatcacc agaaatacaa
     2461 aaataagcac tcccagtgca aaatagtcca ggaacatatt tccctcagag aatataaaaa
     2521 agaacaatta acgcatatta tgaaatgcca tgatgcaatg caatttcctt atttatataa
     2581 cgacatattg ttttttatat actttctgaa gtgtgctgcg tggcttttta acaggtcgag
     2641 gcttatcata ttgaaataat ggaactatca gccaaactaa tgaataactt cagtaaaaca
     2701 tccacaatgc agattaagcg tattaaacct tcaataatct accgtttatt gctgattgtc
     2761 ctcggaatac caatggtgat ttacggtctg gtttgcccgc tcaccatcga aacgcgagat
     2821 taaactcctg gcaaaacacg ccccagaaac gcacttttgt tggcgtagtc tgggttattg
     2881 cacacgtagg tttaaaacgt aaattgtaac aacgtgcgct ttgtttatgc cggatgcggc
     2941 gtgtacgcct tatccggcct acggtcaggt ccccataggc atgataagac gcgtaagcgt
     3001 cgcatcaggc aattaatacc cgatgcgacc agcttcttac atcagaacag ccccaacggt
     3061 ttatctgagt agctcactag caggcacttg gtttgctggt aatgctccag catcatcttg
     3121 tgggtttcgc gaccgatacc tgattgtttg tagccaccaa acgccgcatg tgccgggtaa
     3181 gcatgataac agttggtcca cacgcgccca gcctgtatgc cgcgccccat cttataggcc
     3241 agattaccgt tgcggctcca gacgcccgcg cccaggccat attgcgtatc gttcgccagc
     3301 tccagcgctt cttccatcgt tttgaaggta gtcaccgcca gcaccgggcc aaaaatctcc
     3361 tcctggaaca cgcgcatatt gttctgacca aacagaatcg tcggttcgag gtagtagccg
     3421 tctttcagtt caccttccag cagcttgcgc cgcccgccgg tgagcacatc agcgccctct
     3481 tttttaccga tatcaatgta gttgaggatg gtttccagtt gcccgtgaga aacctgcgca
     3541 cccatttgcg tcacgctgtc gagtgggtta ccgctgcgaa tgctttcgac acggcggatg
     3601 gcgcgttcca taaagcgttc gtagattgac tcctgcacta aggcacgact cggacaggtg
     3661 caaacttcgc cctggttaaa ggcaaacagt gcaaagcctt ccagcgcttt atcgaaaaag
     3721 gcatcttctt catccatcac atcagcaaag aagatatttg gcgacttacc gcccagctcc
     3781 agcgtcaccg gaataatgtt ttgcgtggcg tattgcataa tttgttgacc cacttccgtt
     3841 gagccggtaa acgccacttt ggcgatgcgt ttcgaggtcg ccagatattc gccaatttcc
     3901 ccacctgcac cgttgaccac gttcaccacg cccggcggca gtaaatcacc gacgatttcc
     3961 attagcagca gtacagaaag cggggtaaga cgtgcgggtt tcagcaccac acagttgccc
     4021 gccgccagcg cgggagccat tttccagctc gccatcagca gcgggaagtt ccacgggata
     4081 atctgcccca ccacgcctaa cggttcatgg aaatgatagg ccacggtttc gctatcaact
     4141 tcactgatcc caccttcctg cgcccgaata cacgaggcga aatagcggaa atggtcaatc
     4201 gccagcggta catccgcagc actggtttcg cgaatgggtt tgccgttatc ccaggcttca
     4261 gctgtcgcta acagctcgag gttttgttcc attcgatcgg caatcttaaa cagaatcgcc
     4321 gcgcgatcct gcaccgaggt gtgcgcccat ttatctttca ctttgtgcgc agcatccagc
     4381 gccagatcga tgtctcgttt gcccgaagac gccacttcgc acagcagctg cccggtcacc
     4441 ggcgtcaggt tctggtaata ctcaccatcg gcaggggcta cccattcgcc gccaataaag
     4501 ttgtcatagc ggttttttaa cttgaggggg aaaccatact cgccgggctt aatctgtgct
     4561 gaagggggat tattggtcat gatcgtctcc ttgcggtgtg aggtataaca agggtagacg
     4621 tgactggcga aatcttcgcc agtcggtaac agcttttcga cggctatcac gaatttacgg
     4681 gcaagggtta cagcggataa tgcgcgaagt acacggcttg ctggtcatta attttcttct
     4741 cttgcgcaaa cccttcttta acaattgtcc gtaaatcagc tcgaaccgta ttatcagaga
     4801 cgcccaggct cgcgctaatt tcagcaactg tgtactcttt ccccggattg gcgagcatca
     4861 cattcaggag cgttacctgg cgcggattca ggcgtttaag cacaccagag ctaaacagca
     4921 cactatcgag ttttgcgctt cgttgtgtaa tttcatcgat atgctgctgc cagttttgca
     4981 gagcacgttt aatgacaccc gcctgatatt caaggaaata agtcagatcc ataccatctg
     5041 attcagtgta ctggtagctg gcggcatatt tgaccggtgc agcatgaagc aaacggctga
     5101 tggaaatata tttaaagaca tcgtagcccg atttcagcat ataccagtaa aacagcgccc
     5161 gactggtgcg cccgttgcca tcccgaaacg gatgttcatg ggccagcata aaatgcagaa
     5221 tgcaggcacg gaccaacgga tgaatatacc cttcatggct gtttaaccac tggcaaactt
     5281 tttccagccg ttccggtaac aatgccgcag cgggcggttg atgcactata ttgccatcat
     5341 agtcggcgat cacaacatca tctgtttcac gaaattctcc cggacgatat ttcgcgtcat
     5401 tgattccacc cataccaatg gcatgtaact ggcgaataag cgcaggtgtc agcggttctg
     5461 cgagtaagtg cggaatttcc gccatcagcc gggcatttcc cgcaatcata tgctcgtctt
     5521 cagtgcgtgg cgaccgtcct gtttccagca tttttctcgc caccagcgtt gtagtattcg
     5581 caccttccag ctgggagctg gtgatcggct cttccaggcg taactggctt aactcggctc
     5641 cggccccgcg taaccgttca gtctgatctt tcagcgccag cgcggtagca cgtttgtcga
     5701 caagctcgca cgcttctgca atcaccgggg taatgttgaa tccggcctgt tcacccgctt
     5761 cattgcaata attaattcgt tggatcgccg cgttgcgcgc cagacgggta agcgtccacg
     5821 ccacatcgac gttttctcct tttttgatgc ggtactgcaa ctcatcaaaa ggcagataac
     5881 gtcctttttc atcggtaacc cggttaaagc gcaggtgggc ctggagttcc tcacccgata
     5941 agtgtgataa aggaatcagc gtgggaggtg atttgatagc catttgctta tctcaaaaaa
     6001 gcgcaatatt gagatagtgt atagcaggaa agagaaacta tctcaaaaaa agcaagtttt
     6061 gagatagttg aaaaaatggc tgacaatttg ccagccgttg tggaaatgat gattacatcg
     6121 ctgcgcgata aatcgccaca atttcttcgt gagttgcctg gatcgggtta gtaaagccac
     6181 aggcatcttt cagggcatta gccgccagaa ccgcgaaatc ttcttctttc acgttcaggt
     6241 cgcgtaggcc tgccgggata tccactttct tcgccagttc acggatggcg ttaatgcagg
     6301 cttcagcacc ttccgcgtcg tttttacctg tcacgttcac gcccattgca gcggcacagt
     6361 cacgcagacg tgcagcggcg actttgctgt tgaatacctg aacatgcggc agcagaacgg
     6421 cgttacatac accgtgtggc aggttgtaga aaccgcccag ctggtgcgcc atcgcatgaa
     6481 cataacccag agaagcatta ttgaacgcca taccggcgag gaactgggca taagccattg
     6541 cttcacgcgc tttcgcatta ctgccatctt caacggctaa cggcaggttt tcggcaatca
     6601 tggtcacggc tttcagtgca caagcgtcag tgatcggcgt ggcggcaata gaaacatacg
     6661 cttcgatagc gtgcgttaag gcatccatac ccgttgcggc ggtcagtgac ttcggcatac
     6721 caatcatcag agaggagtca ttgacagaaa gcagcggagt gacatgttta tcaacaatcg
     6781 ccattttgat atgacgcgct tcgtcagtga tgatgcagaa acgggtcatt tcagaggccg
     6841 tacccgccgt ggtattgatg gcgatcatcg gcagctgcgg ttttgcagag cggtcaacgc
     6901 cttcgtaatc gcgaatatcg ccgccattgg ctgccaccag cgcaatacct tttgcgcagt
     6961 cgtgtggaga accaccgcct aaggagatca cgctatcgca attattctct ttaagtaatt
     7021 tcaaacctgc ggcgacgttt tccgtggtgg ggttaggttg ggtgccatca taaataacgc
     7081 taaaaatatt gcgttcttcc agtgcttttt gcacatcgcc cgccatacct aatttcgtta
     7141 acatactgtc agtgacaatt aaggtacggg taaatccata atctgccatc atattcattg
     7201 catcagtcaa tgaatcagcg ccgatgacat tcacagaagg aataaagaaa gttgaagatg
     7261 ccataacaca ctcctgaaag tgtaaaaaag ggtgggcagc ttactgagga ttttcattcg
     7321 aaaacatgat cattattatt aattatcaaa attaatctaa taaaaagcac attgtattca
     7381 ttaaacaatg tgctttttta aaatcataag aaatttgtat taatttattt taaaaattaa
     7441 ttaatcattt ccttattttt ccggaaataa taatgcgtcg cgcaataaat gatcatttcc
     7501 acgacgacgc gtaaagccaa tgcggtcaaa atattccaga atctgaattg ccagctttcg
     7561 gcctacgcct aagcgatcgc ggaaatccgc cgcgcaggtt gaaccacact cctgatcgag
     7621 atcgcggatc atattggcaa attcgacaat ccgatcgtta cggtaataac gatctttgac
     7681 gatcgcggta attattcctt gctgcgccgc ctggcgtagc gtcaggcgca ttgcctgctc
     7741 gtcggtaccc gtctcttttg ccagatcacg cacccaccac ggctcgtcac caaacagcgg
     7801 ctctgctttt tgccagatgg cctgctgctc ttcgctgaag cccgctttgt gatccggcag
     7861 atgcagccag ccgtgatgac tgaggatgtc gccgctttcg cgcatctttt caatcagcaa
     7921 cagtactagc gcttcatctt ccattggcaa cgccatacgt cgcagacgtt cgcgcccagg
     7981 cccaggttca tcgcgatgtt gctcatgata agtcgctaat gtgtcgagaa ttttccgttg
     8041 ccagcgagcg gcaaccggcg cattcaacaa gctataacca gcctgaatat agccaggctg
     8101 ttgcagcagt tcgcgcatcc cttcgccgtt gagctggcgc gcccaggcga aatcaggaag
     8161 attaaccgcg ccgcgttgca ggtgaacggc taacgcttcg ctatcattct gtgccctggc
     8221 gagggaagcc aggcattgca gatactctgg tttacgttta ccgcgacgcg gcgggttaag
     8281 catcacgacg cgcgctccgg ccagcgtgtt gcgggcagag atatcgcgca ataccaggcg
     8341 gtcgttatct gccagccata acggggggtc gaagaccagt tcagcaaggt tatcttccag
     8401 cagtgaaacg cgtcccgtga cgtggctggc ggcgtggtga atatgcagcg gctgccactg
     8461 ggtcagcggt gtatgggttt gaagctcgac aatcacccgt gtgaacggct ctggcggcac
     8521 atcggcaagc agccagtcgc cacggttaat ctgctctttt tccgcatcac ccgcgatgtt
     8581 aagcgcgata cgctgcccgg cattggcggt ttctgttggc tggttttgcg catgcagcgc
     8641 acgtacacgc atcggtttat ttacaccagt cagccagagt gaatcgccta ccttcacttc
     8701 cccgcttaac gccgtaccgg tgacgaccag cccggcacct tttacggtaa atgcgcggtc
     8761 aatcgcgagg cggaaactat gttggctggc gtgctcgcgt tccggcaact gaagcagatg
     8821 ctcgcgcagg gcatcaattc cccgaccttc ggttgctgcg gtgataaaca gttttgcctc
     8881 agcaaaaccg tattcccgca gaacctcctt tacctggcgt tcaacctcat caacacgcgc
     8941 ttcgtctacg cgatcggctt tggtcagcgc cactgtcagc atcgggttac cggtcagctg
     9001 caaaatcgcc agatgctcac gggtctgtgc catcacgccg tcatcgcacg ccaccaccaa
     9061 cagcgcgtga tcgataccac caacgcccgc cagcatgttg gaaagaaact tttcatgacc
     9121 gggtacgtcg ataaaaccag gcacgcgacc atccggctgc ggccagtagg catagccgag
     9181 atcgatggtc atgccgcgct ttttttcttc cggcagacgg tcagcattta cgccagtaat
     9241 cgcctgcaat aaggttgttt tgccgtggtc aacgtgtccg gcagtcgcaa taatcatttc
     9301 aacaacatct ccaaaaaccg ttgctcatct tcaaggcagc gtaaatccag ccacaatcgt
     9361 ccgtcataaa tacgaccaat caccggcact ggcaattcac gccagcgggc ggctaatgac
     9421 tcaaggtggc taccgcgtcc atcatggggt gtaaacgtta atgccgcgct cggcaggcga
     9481 tcaaccggca gcgaaccact gccaatctgc gaaagacatg gcataacctg taccgcaaac
     9541 tccgcgccgt aatgtgcggc aaggggagcc tgtaaacgtt gtgcctggat ttgaatgacc
     9601 tctgcgctgc gggtaagcag gcgcagggtc ggtaattttt cactcagagc ttcagggtgt
     9661 aaataaagac gcaacgtggc ttccagcgcc gcgagggtca ttttatccgc gcgtaatgca
     9721 cgcttcagcg ggtggctttg caggcgggcg atcatctctt ttttaccaac gataatccct
     9781 gcctgcggcc cgcctaacaa cttgtcgccg gagaagctca ccagactgac gcccgccgca
     9841 atcaactcct gcggcattgg ctctttcggc aaaccgtact ggctaagatc gaccagcgag
     9901 ccactgccta aatcagtcac tacgggaaca tccagctctt tgccgagcgc caccagttcc
     9961 gcttcatcta tcgctttggt gaacccctga atgctgtagt tactggtatg tactttcatc
    10021 aacagtgcgg tattttcatt caccgcctga cgataatcat tcgcgtgcgt gcggttggtg
    10081 gtccctactt cgtgtagggt gcagcctgcc tgacgcataa catcgggaat acgaaacgcg
    10141 ccgccaatct ccaccagttc gccgcgagat accaccacct cttttccgct ggcagtggcc
    10201 gccaacatca ataacaccgc cgccgcattg ttattgacga tacaggcatc ttccgccccc
    10261 gtaatacggc acagcagctg cgccagcgcc cgatcgcgat gtccgcgtcc ggcgtcgtcc
    10321 agatcgtact cgagggtcac tggcgaacgc atagcctgcg caacggcttc caccgcggct
    10381 tccgcctgta aagctcgccc aaggttggta tgcagcacgg ttcccgtcag gttgatcacc
    10441 ggacgcagcg cgctctgcgc ttctttcgtc aaccgggcat cgacttcttg cgcccagttt
    10501 tcacaccacg caggcagcgt ctggctgcca cgaatcactt ctcgcgcttc gtcgagcatc
    10561 tgacgcaaca attccaccac gcgggtgtga ccataagtat cacgcaaaga aaggaaggag
    10621 ctatcgcgca ataagcgatc aatagccgga agttgactat agagggaacg cgtttcggtt
    10681 gtcataggaa acctggctga tcaaggccct ctcacacgga gaagggcgtt taacataacc
    10741 acggattgta acgtgagatg ggtcaggagg acatatcgcg catcaagcct ttggcggttc
    10801 ggtgcgagca aaactttcgc ggctaaacag gttttcgacc agtttgacta agtgtgggcg
    10861 gtcgacacac caacctggcg caacgcggcg gaaattgaga tatccgacgg cacaggcaat
    10921 ggcgatagtc gccagattga ccgtatcggt tttgagtgtg ccatcgacca gatatccttc
    10981 cagcacatcc agactgcggt tgattttctc ccgctggcgt aacaattcat cttcagactg
    11041 ctgcgccgct ggacgcgcct gttcacgcac cgataccagc ccggcatcca taatgccatc
    11101 cgccagtgcc tcaattttgc gcacccgcaa cgactccagc ggatcgcgcg gcaacatcgc
    11161 cggagcgaca ttcattaatt caatatattc agcgatgatc ggcgaatcaa accagcattc
    11221 gccctcttcg gtcaccagca ccggcacttt tcctaacggg ttaaattgcg tcacgccgtt
    11281 gtccgcgtta tagggcagtt cattaataaa ttcgaaagtt atgccctttt ctaacaacag
    11341 aatagaaagt ttgcgtacaa acgggctggt gtagctaccg acgagtttca tgccgagtcc
    11401 tttgtgcgag gaaaaatatc agtatggcct ggtgatggga aaaagggcag aaaatgttga
    11461 tggtttgtta cttccaaata aatcacatat ttatcatggt gatataaata ttttcctaat
    11521 tgtttctatc tgatggatat ctcacttaag gctttcttat aaatctgtag ggttttgcct
    11581 ggaagcagac aaataacccg ataaaacaag gatgagcaga tgagcggaaa accggc
//
LOCUS       DAZFBR010000130        11598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFBR010000130 DAZFBR010000000
VERSION     DAZFBR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.130"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            430..972
                     /gene="ecpR"
                     /locus_tag="ACHJZ2_004087"
                     /gene_synonym="matA"
     CDS             430..972
                     /gene="ecpR"
                     /locus_tag="ACHJZ2_004087"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO4570101.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSETLANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            1047..1634
                     /gene="ecpA"
                     /locus_tag="ACHJZ2_004088"
                     /gene_synonym="matB"
     CDS             1047..1634
                     /gene="ecpA"
                     /locus_tag="ACHJZ2_004088"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO4570102.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1692..2360
                     /gene="ecpB"
                     /locus_tag="ACHJZ2_004089"
                     /gene_synonym="yagY"
     CDS             1692..2360
                     /gene="ecpB"
                     /locus_tag="ACHJZ2_004089"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO4570103.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGTLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2386..4911
                     /gene="ecpC"
                     /locus_tag="ACHJZ2_004090"
                     /gene_synonym="yagX"
                     /pseudo
     CDS             2386..4911
                     /gene="ecpC"
                     /locus_tag="ACHJZ2_004090"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
     gene            4901..6544
                     /gene="ecpD"
                     /locus_tag="ACHJZ2_004091"
     CDS             4901..6544
                     /gene="ecpD"
                     /locus_tag="ACHJZ2_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO4570104.1"
                     /translation="MRVNLLIAMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDTSGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNQGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6513..7223
                     /gene="ecpE"
                     /locus_tag="ACHJZ2_004092"
     CDS             6513..7223
                     /gene="ecpE"
                     /locus_tag="ACHJZ2_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO4570105.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPEDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7536..7865
                     /gene="ykgJ"
                     /locus_tag="ACHJZ2_004093"
     CDS             7536..7865
                     /gene="ykgJ"
                     /locus_tag="ACHJZ2_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4570106.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(8113..8727)
                     /gene="yagU"
                     /locus_tag="ACHJZ2_004094"
     CDS             complement(8113..8727)
                     /gene="yagU"
                     /locus_tag="ACHJZ2_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO4570107.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            9145..9834
                     /gene="paoA"
                     /locus_tag="ACHJZ2_004095"
     CDS             9145..9834
                     /gene="paoA"
                     /locus_tag="ACHJZ2_004095"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO4570108.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9831..10787
                     /gene="paoB"
                     /locus_tag="ACHJZ2_004096"
     CDS             9831..10787
                     /gene="paoB"
                     /locus_tag="ACHJZ2_004096"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO4570109.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLVAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10784..>11598
                     /gene="paoC"
                     /locus_tag="ACHJZ2_004097"
     CDS             10784..>11598
                     /gene="paoC"
                     /locus_tag="ACHJZ2_004097"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO4570110.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLPVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPR"
BASE COUNT         3014 a         3025 c         2953 g         2606 t
ORIGIN      
        1 atttagactt gtttttttac tagtccatta cacaacacat taagactatt cctaacacct
       61 cagggcaaag ttcctggcta atataaaatg caagtaagaa ttgaacgtta tattgccaat
      121 aaccttatga aactgaatgt ctttttcttc ttatcaaaaa agcaatattt tcattttttg
      181 taaatattga cttaaccatg gaattcattt tctgttcaca tattgacact catcaggaaa
      241 aaaacataaa tttaaaccta atcgaaataa ttaaaaactt aatctcgttt aacctatatt
      301 gatatgtgct acgtatctta tttacttccg atttactaaa gaaactgaat gtacctgtaa
      361 aaattacagg tttggaaagt agtgacatgg caaaatgatt acagcaggga ctatgaggtt
      421 gaaaaccata tggaatgtca aaaccgttct gataaataca tctggtctcc ccatgacgcc
      481 tacttctata aaggactatc tgaactgatt gtggatatcg acagattaat ttatctatcg
      541 ctggagaaaa tcagaaaaga tttcgtgttt atcaatctca atacggattc tttaactgaa
      601 tttataaacc gtgataatga gtggttatcc gcggtaaagg ggaaacaggt cgtattgatt
      661 gcggccagaa agtcagaaac cttagcaaat tattggtatt acaacagcaa tattaggggc
      721 gtggtatacg ctggactgag tcgtgatatt agaaaagaac tggcctatgt gattaatggc
      781 aggttcctga gaaaagatat taagaaagat aaaatcactg accgggaaat ggaaattatc
      841 cgcatgacgg ctcagggaat gctgcctaaa tcgattgcca gaattgaaaa ttgtagtgta
      901 aagacagtgt atacccatcg gcgtaatgca gaggccaagc tgtactcaaa attatataag
      961 ttggttcagt aaactccagg caagttagtt ttaaaaaatg actcactggg acatcacgtc
     1021 ctcaattcaa ctcgggaaga aaaacaatga aaaaaaaggt tctggcaata gctctggtaa
     1081 cggtgtttac cggcatgggt gtggcgcagg ctgctgacgt aacagctcag gctgtagcga
     1141 cctggtcggc aacagccaaa aaagacacca ccagtaagct ggttgtgacg ccactcggta
     1201 gcctggcgtt ccagtatgcc gaaggcatta aaggttttaa ctcacagaaa ggtctatttg
     1261 acgtggctat cgagggtgac tcaacggcta ccgcctttaa actgacctca cgtcttatca
     1321 ccaacacatt aacccagttg gatacctcag gttccacact gaatgtgggc gtggattata
     1381 acggcgcggc agtcgaaaaa actggcgata ccgtgatgat cgataccgcc aacggcgtac
     1441 tgggcggcaa ccttagcccg ctggcaaacg gttacaatgc cagcaatcgt accaccgcac
     1501 aggatggttt cactttctcc atcatcagcg gcaccaccaa tggtaccacc gcagtaaccg
     1561 attacagcac tctaccggaa ggcatctgga gcggcgacgt tagcgtacag ttcgacgcga
     1621 cctggaccag ttaatctctc tgatgtacca gcaggggtag ccccccagct ttattcctgg
     1681 acggaattgt tatgaaaaag caccttctgc ttctcgctct gctgttatcc ggaatatctc
     1741 cggcccaggc gctggatgtc ggcgatatat catcgtttat gaacagtgac agcagcacgc
     1801 tgagcaaaac gatcaaaaac agtaccgaca gtggtcgcct tatcaatatc cgtctcgaac
     1861 ggctctcttc accgcttgac gacgggcagg ttatctcaat ggacaagccg gacgagttgc
     1921 tactcactcc cgccagcttg ctgctacccg cccaagccag cgaagtgatc cgcttcttct
     1981 ataagggacc agcagatgaa aaagagcgct actaccgcat tgtctggttt gatcaggccc
     2041 tcagtgatgc gcagcgcgat aatgccaacc gcagcgctgt ggccactgct tccgcccgca
     2101 tcggcaccat tctggtcgtc gccccccgcc aggcaaacta ccactttcag tacgccaacg
     2161 gcaccctgac aaatacagga aatgcgacgc tgcggatcct cgcctacggc ccctgcctga
     2221 aagccgccaa tggtaaagag tgtaaagaga attactacct gatgccgggc aagtcgcgtc
     2281 gttttacccg cgtggacacg gcggataaca aaggacgggt tgcactttgg cagggtgata
     2341 agttcattcc cgtgaaatag atagctgtgc agatggataa cgacaatgcc tttacgacgg
     2401 ttctccccag gactgaaagc ccagtttgcc ttcggcatgg tctttttgtt cgttcagccc
     2461 gatgccagcg ctgctgacat aagtgcgcag caaataggtg gggtgattat tccgcaggcc
     2521 ttcagtcagg cgcttcagga cggcatgagc gtcccgctct atattcatct cgccggtagc
     2581 cagggtcgcc aggacgatca gcgaatcggc agcgctttta tctggttgga tgatggacag
     2641 ctacgcatcc ggaaaataca gctggaagag agtgaagata acgccagtgt cagcgaacaa
     2701 actcgacagc agctgatggc tctggcgaac gccccgttca atgaggccct taccatcccc
     2761 ctgactgaca acgcgcagct ggatctcagc ttgcgccaac tgctgctgca gctggtggtc
     2821 aagcgcgaag cgctgggcac tgtactacgc tcacgtagcg aagacatcgg gcagtccagt
     2881 gttaacaccc tcagcagtaa tctgagctat aacttcggca tctataacaa ccagttgcgt
     2941 aacggcggga gcaacacatc cagctatctg tcgctgaata acgttactgc actgcgctaa
     3001 catcatgtgg tgctcgacgg ctcgctgtac gggatcggta gcggtcaaca ggacagtgaa
     3061 ttatataaag cgatgtatga acgcgatttt gccggtcacc gatttgccgg tggaacgctc
     3121 gacacctgga acttgcagtc cttagggccg atgaccgcca tttcagcagg gaagatttac
     3181 ggcctttcct ggggaaacca ggccagctcc accatcttcg acagcagcca gtcagccacg
     3241 ccagtgatcg cctttttacc ggcggcgggt gaagtacatc tcacccgtga tgggcggtta
     3301 ctaagcgttc agaacttcac catgggcaat catgaagtgg atacccgggg tctaccatac
     3361 ggtatttacg atgtggaagt tgaggtgatc gttaacggtc gcgtgatcag caaacgcacc
     3421 cagcgggtca ataagctgtt tagccggggg cgcggcgtcg gtgcaccact ggcgtggcag
     3481 gtatggggcg gtagctttca tatggatcgc tggtcggaaa acgggaaaaa gacgcgacca
     3541 gctaaagaga gttggctggc aggtgcctcg acctccggct cactgagtac gcttagctgg
     3601 gcggcaacgg gatatggata cgataatcag gcggtgggtg aaacccgtct gacgctgccg
     3661 cttgggggag cgatcaacgt taacctgcaa aatatgctgg ccagtgacag ctcatggagc
     3721 agcatcggca gcatcagcgc cactctaccg ggaggcttta gttcgctgtg ggttaatcag
     3781 gaaaaaaccc gcattggcaa tcaattgcga cgtagcgatg ccgacaaccg tgctatcggc
     3841 ggcacactca acctgaactc actgtggtcg aagctgggca cattcagcat cagctacaat
     3901 gatgaccgcc gttacaacag ccattattac acggcagatt actatcaaaa tgtctacagc
     3961 ggtacctttg gttcgcttgg cctgcgggcc ggtattcagc gctataacaa cggcgacagc
     4021 aacgccaata cagggaaata tatcgctctc gatctctcgc taccactggg caactggttt
     4081 agcgcaggga tgactcatca aaacggctac accatggcaa acctgtcagc acgcaagcag
     4141 tttgatgaag ggaccattcg cactgttggt gccaatctgt cacgagccat ctccggcgat
     4201 accggtgatg acaaaactct cagtggtggg gcgtatgcac agttcgacgc tcgctacgcc
     4261 agcggaacgc tgaacgtcaa tagcgcggcg gacggctacg tcaataccaa tttaaccgcc
     4321 aatggcagcg tcggctggca gggtaaaaac attgctgcca gcgggcggac tgatggcaac
     4381 gctggggtga tattcaacac cgggctggag gacgacggtc agatcagcgc caaaatcaac
     4441 gggcggattt tcccgcttaa cggcaagcgt aactatctcc cgctctctcc ctatggaaga
     4501 tatgaggtgg agttacagaa cagcaaaaac tcactcgaca gttacgatat cgtcagcggt
     4561 cgcaaaagtc atctgactct ctatccaggc aatgtcgctg tcattgagcc agaggtgaag
     4621 cagatggtta ccgtctccgg tcgtatccgt gcggaagacg gcacactgct ggctaacgca
     4681 cggattaaca accatatcgg ccgaacccga accgatgaaa acggcgagtt tgtcatggac
     4741 gtggataaga aataccccac tatcgatttt cgctacagtg gcaataaaac ctgcgaagtg
     4801 gcactggaac tcaaccaggc gcgcggtgcc gtctgggtcg gtgatgtggt ctgcagcggc
     4861 ctctcatcgt gggcggcggt gacgcagaca ggagaagaga atgagagtta acctactgat
     4921 agcgatgata atctttgcgc taatctggcc agtaactgcg ctcagagcgg cagtgagcaa
     4981 aacaacctgg gcggatgcac cggcacgcga gtttgtgttt gtcgaaaaca actcagacga
     5041 caactttttc gtcactcctg gcggggcgct ggatccgcgc ctgaccggtg ccaaccgctg
     5101 gaccggttta aaatacaatg gttcaggaac catctatcag caaagcctcg gctacattga
     5161 taacggttac aacaccggcc tttataccaa ctggaagttt gatatgtggc tggaaaattc
     5221 accagtttca tctcctttaa ctggcttgcg ctgcatcaac tggtacgctg ggtgtaatat
     5281 gaccaccagt cttatcctgc cgcaaaccac cgacaccagt ggattttatg gcgcgacggt
     5341 gaccagcggc ggcgcgaagt ggatgcacgg catgttgtca gacgcgtttt accagtatct
     5401 gcaacaaatg cccgtcggca gcagctttac aatgaccatc aatgcctgcc agacctctgt
     5461 gaactatgac gccagcagcg gcgcacgctg taaggatcag gcctccggca actggtatgt
     5521 tcgcaacgtc acccatacga aagcagcaaa tctacggttg ataaataccc actcgctggc
     5581 ggaagtattt atcaacagcg acggagtacc gactctgggc gaagggaacg ccgactgccg
     5641 gacgcaaacc atcggcagcc gttcaggatt aagttgtaag atggttaact ataccctgca
     5701 aacaaacgga ctcagcaaca cctcaatcca tatattcccg gcgatcgcca actcgtcgtt
     5761 agcctcggcc gtcggggcgt acgatatgca gttcagtctg aatggcagtt catggaaacc
     5821 ggtgagcaat actgcctatt actacacctt caacgagatg aagagcgcag actcgatcta
     5881 tgttttcttc tcgagcaact tctttaagca gatggtgaac cagggaatca gcgatatcaa
     5941 caccaaagat ctattcaact ttcgctttca gaacaccaca tcaccggagt ctggctggta
     6001 tgaattttct acctccaaca cgctgattat caaaccccgt gatttcagca tcagtattat
     6061 ctccgatgaa tatactcaga caccgtcgcg ggagggatat gtcggcagcg gcgagtcggc
     6121 actcgatttc ggctatatcg taaccaccag cggtaaaaca gctgccgacg aagtgctgat
     6181 caaggtgacc ggacccgcgc aggtgattgg cgggcgctcc tattgtgtct tcagctccga
     6241 tgacggtaag gcgaaagtac cgttcccggc gacgctttcc tttattaccc gcaacggagc
     6301 tacaaaaacc tacgatgccg ggtgcgatga tagctggcgg gatatgaccg atgcgctgtg
     6361 gttgaccaca ccgtggactg atatctctgg cgaagtgggg cagatggata agaccacagt
     6421 caaattttcg attccaatgg ataacgccat ttctctgcgt acggtagatg ataacggctg
     6481 gtttggcgaa gtcagcgctt caggagaaat tcatgttcag gcgacgtggc gtaacattaa
     6541 ctaaggccct gctgacagcg gtctgtatgc tggcggcacc tttgacacag gcgatttcgg
     6601 tcggcaatct gactttttca ctgccgtccg agactgactt tgtcagcaaa cgtgtagtga
     6661 ataacaacaa aagcgcgcgg atataccgta ttgccatcag tgctatcgat agcccgggca
     6721 gcagtgaatt acgcacccga ccggtggatg gtgaactgct tttcgccccc cgccagctgg
     6781 cgttgcaggc tggtgagagc gagtatttta aattttacta tcatgggcca caggataacc
     6841 gcgagcgcta ctaccgggtc tcatttcgcg aggtccccac tcgtaacctg acaaaacgca
     6901 gccctaccgg cggtgaggtt agcacggagc cggtggtggt gatggatacc attctggtag
     6961 tacgaccacg tcaggttcag tttaaatggt ccttcgatca ggtgaccgga acggtaagta
     7021 acaccggcaa cacgtggttt aagctactga ttaaaccagg atgtgattcg accgaagagg
     7081 aaggcgatgc ctggtatcta cgtccggaag acgtggttca tcagcctgag ttacgtcagc
     7141 cggggaatca ctatctggtc tataacgaca aattcattaa gattagcgat tcctgtccgg
     7201 ctaagccccc ttcggcggac tgatacttat ccatcggtcg agggaagaat tccacctcag
     7261 agactcaaaa atcgtttaaa tgatgtggaa gcgatcgtca atatcacctg taacgatagg
     7321 ctttctcata tgcacaccac agtgttcagg agacacggaa aggtaccagg cggcctgcta
     7381 acgagtggtt tcttcactaa ttaggacgta aaatcggtaa cggctggaaa tcattcaata
     7441 ctcacactat cgaaagtaca ccagccaacc gcgatacgtc cctgcatacg acgtaccgca
     7501 gttctcttta cgatttatag ctgtactggt gaattatgag caatctgaat ccatgcatga
     7561 cgtgtggtgc ctgttgtgca tttttccgcg tctctttcta ctgggccgaa gccgacgatg
     7621 ctggcggcac tattcccgcc aggctcactg aacaaatatc cccttttcac cgatgcatga
     7681 gcggaactaa tcagaaaaac ccccgatgta ttgcccttgc aggaaccccg ggcaaaaatg
     7741 cctgctgcac gatatataaa aatcgatcgt ccacatgcag agaattcgcc atgtctggtg
     7801 agaacggagt cgtcaatgag gcttgcaatc gtgcaagggc taaatacggg ctgacaccgt
     7861 tataaatata caataattat ttgcactgcc ccgccagctg taatagcggg gcttcgtttt
     7921 tcaggggtaa caaaccccga tacttcttct atttgccagc aacaatgcct ctcttctgta
     7981 gcgttcttcg gatctacatc atcctgaagt agcgcgatat actcactgac tatctgaatc
     8041 agaatataaa aagcaatgtt tttaacctat aaaaatggcg ctgtatttgc gccattttta
     8101 tcattcaatg cattatctgt ttgagcctaa agggatctca gggtctggct catgagtaat
     8161 tctgtttcgt aaatctctgc gaataatttc aatggaccag aaccagacta aatgtccaaa
     8221 aatttcagaa acattctcat accacgggag atcaaacaga ggtggtgtca gtcccatgag
     8281 agggaatgaa atcatatgaa caaaaagttg ggctaaagca cctgccagta agccctgcca
     8341 gagtttaatt tttggaaata cttctgcgac cacacaataa ccgacagcaa acactatcga
     8401 aaagataatg tgcgtaacac caacccagtt aaagacatgc ccggcaaagg tataaacagc
     8461 cgcattggga tctgtcagcc ccagccagtc tcgaagaaaa atatacggag gattgagaaa
     8521 attacgcgag caatcaattt gcccagcagc cctgattaat gattccgggc cacacgctgc
     8581 attaaacata tccaccgggc tacgtggcgg caatggaact tcagcccccc acttcacaaa
     8641 tgcggaaaca acgccagcaa tcagcccaat gaatgcagca agaccataac gtctgcggtt
     8701 cggtggagtt tgttcaaata tattcatatc taccctgctt gtaccattat gttatacacc
     8761 tctttaggag tattcataaa acaaggtaaa tgtaaagaac tgtattgttt tgtataacaa
     8821 gatggttgtc taatcaccaa agaatataag caccatcatt tctccctatt tttatattag
     8881 aagtgacata gatttgcagg gtgatgcaga gctgacaatc acaggtttcc ttatcggttt
     8941 ttgcatcgta caactaaagc aataaaccag ctcccatcat catcaacatc ccggcgaaca
     9001 tgaatttatt agccgggctc atagctgcat tcgcgtttta agaataatcc tcctgctgtc
     9061 gccgactatg cttaacgttt aaaaaagcat cagcactctc gcaacgcact cttattttcc
     9121 cctttagaat accggaggcc tggtatgagc aaccaaggcg aataccccga agataatcgg
     9181 gttgggaagc atgagccgca cgatttaagt ttgacccgtc gcgatctgat taaagtgagc
     9241 gccgcaacgg cggcggccgc cgtggtttat cctcattcta cgcttgcggc aagcgttccg
     9301 gcagctacac ccgcgcctga gataatgccc ctgacactta aggtgaacgg caaaaccgag
     9361 cagcttgagg tggatacccg aaccacgcta ctggacgctt tgcgtgaaaa tctgcatttg
     9421 atcggtacca agaaaggttg cgatcacgga cagtgcggag cctgtaccgt gctggtcaat
     9481 ggtcgcaggc ttaatgcctg cctgacgctt gcagtcatgc atcagggggc cgagatcacc
     9541 accattgaag gcctgggttc accagataat cttcacccca tgcaggcggc ctttatcaag
     9601 catgatggct tccagtgcgg ctactgcacc tccgggcaaa tttgctcatc agtagcggtg
     9661 ctaaaagaga ttcaggacgg cattcccagt cacgtcacgg tcgatttggt ttcccctcca
     9721 gaaagaactg ccgatgagat ccgtgaacgt atgagcggca acatctgtcg ctgtggtgca
     9781 tacgctaaca tccttgccgc cattgaagac gctgcggggg agataaaatc atgaaggcgt
     9841 ttacctatga acgagtgaac acccccgccg aggcggcact tagcgctcag cgcgtacccg
     9901 gcgcaaaatt tatcgcgggc gggaccaatc tgctggacct gatgaagctg gaaattgaaa
     9961 cgcccaccca ccttatcgat gtgaacgggc tcgggctcga taagatcgaa gtgaccgacg
    10021 cgggtgggct gcgcatcggc gcactggtac ggaacaccga cctggtggct cacgagcgcg
    10081 tgcgtcgtga ttacgcggta ctctcccgcg ccctgctcgc tggcgcgtct ggtcagttac
    10141 gcaatcaggc aaccacagca ggtaatctgc tccagcgcac gcgctgcccc tatttttacg
    10201 acaccaatca gccctgcaat aagcgcctgc ccgggagcgg ctgcgcggcg cttgaaggct
    10261 ttagccgtca gcacgcggtg gtaggcgtaa gcgaagcctg cattgccacc catccgagcg
    10321 atatggcggt cgcaatgcgg ttgctggatg cggtggtgga aaccatcacg ccggagggaa
    10381 agactcgcag tatcacactg gctgattttt atcacccacc ggggaaaacg ccgcacattg
    10441 aaaccgccct gcttcccggt gagcttatcg ttgcggtgac gttacctccg ccgctcggcg
    10501 gaaaacatat ctaccgtaag gtgcgcgatc gcgcctccta cgcctttgcc ctggtatcgg
    10561 tcgcggcgat tattcagcct gacggcagcg ggcgcgtcgc gctgggcgga gtagcacata
    10621 agccctggcg cattgaggct gcggatgctc agctatccca gggggcgcag gccgtatatg
    10681 acgcgctgtt cgccagcgcc catcccaccg ctgaaaacac ctttaaactc ctgttggcga
    10741 agcgaacgct tgcctccgta ctggctgaag cgagggcaca agcatgaaat ttgataaacc
    10801 cgcaggggaa aacccgatcg atcagctgaa ggttgtcggt cgtccccatg accgcatcga
    10861 cggaccgctg aaaactaccg gcacggcacg ctacgcctac gaatggcatg aagaatcccc
    10921 caacgccgcc tatggctata tcgtcggttc cgccattgcc aaaggacgcc tcaccgccct
    10981 tgatacggac gccgcgcaaa aagcgccggg cgtactgcct gtcattaccg ccagtaacgc
    11041 cggggcactc agtaaaggcg acaaaaacac cgccaggctg ttaggcggcc ccactattga
    11101 gcactatcat caggccattg ctctggtagt ggccgagacc ttcgaacagg cgcgagcggc
    11161 ggcctcgctg gtgcaggcgc actatcgccg taataaagga gcttactccc tggcggacga
    11221 aaaacaggcc gtcagtcagc cgccggaaga cacgcccgac aagaacgtcg gtgactttga
    11281 cggggctttc tcctccgctg cggtgaagat tgatgctacc tacacgaccc cggaccagag
    11341 ccatatggcg atggagccgc atgcctcgat ggccgtctgg gatggaaata agcttactct
    11401 ctggacctca aatcagatga ttgactggtg ccgcaccgat ctggcaaaaa cgctgaaagt
    11461 gcccgtggag aatgtgcgta ttatctcccc gtatatcggc ggagggtttg gcggcaagct
    11521 gttcctgaga agcgatgcgc tgctggcggc cctcgccgcc cgagcggtga aacgtccggt
    11581 taaagtgatg ctcccccg
//
LOCUS       DAZFBR010000131        11553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFBR010000131 DAZFBR010000000
VERSION     DAZFBR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.131"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..695)
                     /locus_tag="ACHJZ2_004098"
     CDS             complement(<1..695)
                     /locus_tag="ACHJZ2_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4570111.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIALPPHTHQQ"
     gene            complement(697..969)
                     /locus_tag="ACHJZ2_004099"
     CDS             complement(697..969)
                     /locus_tag="ACHJZ2_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570112.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            complement(1091..1435)
                     /locus_tag="ACHJZ2_004100"
     CDS             complement(1091..1435)
                     /locus_tag="ACHJZ2_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO4570113.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            complement(1555..1710)
                     /locus_tag="ACHJZ2_004101"
     CDS             complement(1555..1710)
                     /locus_tag="ACHJZ2_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4570114.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(2001..2558)
                     /locus_tag="ACHJZ2_004102"
     CDS             complement(2001..2558)
                     /locus_tag="ACHJZ2_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4570115.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            complement(2560..2778)
                     /locus_tag="ACHJZ2_004103"
     CDS             complement(2560..2778)
                     /locus_tag="ACHJZ2_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO4570116.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            complement(2906..3217)
                     /locus_tag="ACHJZ2_004104"
     CDS             complement(2906..3217)
                     /locus_tag="ACHJZ2_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570117.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            complement(3210..3437)
                     /locus_tag="ACHJZ2_004105"
     CDS             complement(3210..3437)
                     /locus_tag="ACHJZ2_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570118.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            complement(3434..3715)
                     /locus_tag="ACHJZ2_004106"
     CDS             complement(3434..3715)
                     /locus_tag="ACHJZ2_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4570119.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            complement(3748..4423)
                     /locus_tag="ACHJZ2_004107"
                     /pseudo
     CDS             complement(3748..4423)
                     /locus_tag="ACHJZ2_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(4457..4918)
                     /locus_tag="ACHJZ2_004108"
     CDS             complement(4457..4918)
                     /locus_tag="ACHJZ2_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4570120.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(4911..5960)
                     /locus_tag="ACHJZ2_004109"
     CDS             complement(4911..5960)
                     /locus_tag="ACHJZ2_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO4570121.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKHNADCHDESRRVTHLSRQVTTDKDTDKD
                     TDTDTELNPTHNARESIPTSESNGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFR
                     QRAAVWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVS
                     KGGSHAASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAHGGGVFEPLDSEER
                     GRTFEALDCPDWCDD"
     gene            complement(6029..6454)
                     /locus_tag="ACHJZ2_004110"
     CDS             complement(6029..6454)
                     /locus_tag="ACHJZ2_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4570122.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDAVEKMRALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(6438..6680)
                     /locus_tag="ACHJZ2_004111"
     CDS             complement(6438..6680)
                     /locus_tag="ACHJZ2_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4570123.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEDVPWKWALLIQA
                     VTAGALKYELHIPTVVIPDSDHNPPSNQGGIHENQA"
     gene            7072..7410
                     /locus_tag="ACHJZ2_004112"
     CDS             7072..7410
                     /locus_tag="ACHJZ2_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO4570124.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRIVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            7703..7855
                     /locus_tag="ACHJZ2_004113"
     CDS             7703..7855
                     /locus_tag="ACHJZ2_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4570125.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
     gene            7867..8505
                     /locus_tag="ACHJZ2_004114"
     CDS             7867..8505
                     /locus_tag="ACHJZ2_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570126.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(8506..8715)
                     /locus_tag="ACHJZ2_004115"
     CDS             complement(8506..8715)
                     /locus_tag="ACHJZ2_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570127.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            9280..9468
                     /gene="dicB"
                     /locus_tag="ACHJZ2_004116"
     CDS             9280..9468
                     /gene="dicB"
                     /locus_tag="ACHJZ2_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4570128.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            9465..9653
                     /locus_tag="ACHJZ2_004117"
     CDS             9465..9653
                     /locus_tag="ACHJZ2_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4570129.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            9746..10984
                     /locus_tag="ACHJZ2_004118"
                     /pseudo
     CDS             9746..10984
                     /locus_tag="ACHJZ2_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
     gene            10972..11523
                     /locus_tag="ACHJZ2_004119"
                     /pseudo
     CDS             10972..11523
                     /locus_tag="ACHJZ2_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         2935 a         3010 c         2606 g         3002 t
ORIGIN      
        1 tgttgctggt gggtgtgagg cggtagcgca atattttttg tgcgctgctt cagtatgctg
       61 gtggcggtct gttctcccgg tatgatgtcg ctttcacggt atacggagcg gattttttcc
      121 gccggtaatc ccagagagcg acgcgatact gcctcaggta atgcgtccac cacctgattg
      181 cagactgccc accaggataa ttcagccagc gataattccc gctcctgcgt gccattcatt
      241 acatggcgta tgacgtcaat catccaggct gtcagatttt gttgagtaag ttgctcaagt
      301 gattcggagg tctggtcgcg cagctggttg tcgcagtgcc agcacaacac cattgcgcca
      361 gcaccgtaac tgtgaataac ggtttcgctg tgatgataat cgccgtgtgg ccactggcag
      421 gatttcacgt gacgtaataa ccagtcagac agtgcaccag cgccgccagc tgcacgaatc
      481 acccgctcat cgctgaaaaa tggcagcagt gatttatctt ccgccagcgg ctggcgaaca
      541 gcaggaaccg ctccagatgg cagcgcccgc atgtttttcg gttccggctc caccagcacc
      601 ctgccgcgat gaaaaactgg caatgattca cgaccgggct taaggaccac cagcccgagt
      661 tccggaacca gaacaggtcg aagtaatacc cgcacattac ctccagacgc gctgacggta
      721 gcaggcatgt gtccgtggca gatgtgcacg aacaggaaga tatacagaaa cggtccaggt
      781 cagacgatca gcgttcagac tccgctccac acggacaccg cgacgcagat acgcctcttg
      841 aagcatatct gcctcatcga tcgtacagaa cagatagtga aaccagccat actgaggcgc
      901 acgaaaacgc ctcccctgct taatttccgg gtcggcttca gaattgtggg attttatgtg
      961 ttgtgtcatc ggattctccg gtgacagcag gtgtcagttg ttcaggctga ctgcgcgaat
     1021 tgtaaggcaa tacgccggaa tgtacaaaca gaaaacccgt cagtaagacg ggcttaacaa
     1081 gcaggggcgg ttactttaat aatttcagtg cctttacatc aacttcaaca ctgctcaggt
     1141 ctttatcaat ttcaccctca attcttactt tgtctttcgg agaaacattc tgcccggccc
     1201 atacgctgtc atcaatatcc gtgacaattg tcccgctatt gtcacgaaac tcataacgtt
     1261 catcacccac ttttttaacg atgctccctt caaggataac ccatgcatca tccttcagtt
     1321 cttttgcctg cgctactgtt gaacgctctg cttctggccc ctggaaacca ccctgctgtg
     1381 caaaagcgcc aaaagacaca ccggaaataa gtgctgcaat caataccttt ttcattcata
     1441 gtcctctttc agagatgaac attcaaacag cattttcagt atggtaaagc gcgggtgcgt
     1501 tgaggatgcc tgacacatca gaggtggcgg gagattactc ccccgcctgg tctcttactt
     1561 ctcagattcg tagtctacga agacagcgac ctccgtctga ccggttcgga ttcgcacctc
     1621 gcagaggtct ttcctcgtta ccagtgccgt cactatgacg gttaaacaga tgacgatcag
     1681 ggcgattaac atcgcctttt gctgcttcat agcctgcttc tccttgcctt tcggcacgta
     1741 agaggctaac ctacatgtgt tcagcatgga ttgagcctca gattaatgtt aagcgtcttg
     1801 caggacgcgt aatgttaact ggggcttttc tctatctgcc gttggtgttc atgcccgagg
     1861 cagatagcct caagcacccg cagcaattct acttaactat ccttttcccg caaaccgttt
     1921 ttatccccag cggcaaatcg aatacaccac cagcgccacc gccatcgcaa ttcctaccgt
     1981 tgtgaatgct tcaggccagg tcattgattc acctcctgcg gcggttctgg tagcgacatc
     2041 cagtgggttg cttgcctaag atcattaccc ggactaactg ctatacctcc gcgccggaac
     2101 gtgcctccga gatagcgtgc ggaatatatt aatggcccag cctcgctatc gatattcatc
     2161 gaaataagca cgttctggct cttttcaggc attcgctcag tacagcttat ccagccatcc
     2221 agagttatcg gagatctggt tgacgtttcc gagatttccc gaaaattgtt ggttgacgaa
     2281 ttcttatttt cccgaaagtt tccggcctga agcatggcga cgcggcaggc gttccagcca
     2341 tcagcatatg ttttagttac accgtcgaga tggcaggtaa gcaaatccat ttcatcaggc
     2401 actaccattg ctatcggctc tgcttccagt gatgccagcg cgatacgaaa cacattagcc
     2461 agcaggctgt ctgaagactt gttatcgtgc gccgagtcgc tcaggaagcc tgtgatgtat
     2521 gatttaatct ccgcgcgttc tctggtaata gtgctcatat cagttttcct tatacggatt
     2581 aattttattg tgcagtgcgc tgaatgattc ccatgtcaca tcggtatata gctcaataac
     2641 tggttcaaat gtccttccaa ttatccagac cagtaatagc ggggatatcg gtatcatcaa
     2701 cactataaac agaatgaaaa acagaaactc tgttgttctg ctctttcgtg ggtaattttt
     2761 ttctaaataa tgtttcattt cttaccgccc tttcgggcgg cctcctgatg ttctgagggt
     2821 gcaggaatcc ctccggttaa ggatttaata aaaatcgttt ctgatttaaa tcttcagtat
     2881 ttagttgtta gttggtttat cgcctttatg cttcagcctt atttcgcaac cagacacaaa
     2941 ccgggccatc ttccgtatca tgaatggaac caataaacca gccatcgccc tctggtcgtt
     3001 ccggttccca tgcagaaata tcagcatcac acgcatcaag gtcagcacat ccttcatctc
     3061 tgaagcagag gacgtattga agattatttt cctccatcca ggcgttaaac tcttccgttg
     3121 aaatatattc ccgaccatca cagaattttt catattcagg atgtgtccag tagccatatc
     3181 cgtcacgaac taccggcatt tctttaattt cactcactgt taacctcctg caacgctaca
     3241 cgatacgcct tctttatcca cgccttactg ccatataatt tcgtcttcat aataaacaca
     3301 cctgcacgac tcgccgatat ccccggacag gttaacagca cagcatccac cacacggtta
     3361 tgcttccgaa actccattac agtactgctg ataaccacct gccccaccgg gccgtaatcc
     3421 tgatacagga ttttcacgca gacaccctcc tgtcgaaata aacgtagtta ttcactatgc
     3481 gcagcggcat gcctaatttt ctggcaattt cccttctttg catgcctctc tgatgcagtt
     3541 gccgcgccag ctcaatatca cgctgagaat attttgccga cgggtgaaaa tcaccacgta
     3601 acatcatgct gatgcccagc tcccgtgcct tcgttctcac tgccgcttca gttcgtccga
     3661 taagtgcgcc aacgcttttt accttcatcg ttcccgcaca ctgccggagt atcatgattt
     3721 ccgcccggca ccacgtcttc cacccactca ccgctgctgt tctctggtgg cggtaatatc
     3781 ccggagaata tcccggcact tgttcagctc ccgcagcgcg gcgcagctcg cggttagcca
     3841 catgcagcga tggtaaaatc agaccatccg gatgctttct ggtgaacgac ggctgtgact
     3901 gcactgtgac cgccacactt tccgttttta tttcttcctg tgtttccgct tccccgactg
     3961 gtaacgcaac acctgctggc tgaggaaagg ctttaccatc cgtttccgtt atggatgcgg
     4021 ctttcggctc tgccggtaaa tcagcgcccg gtatgcagta ccgaaattta ccgccctgat
     4081 tcacgcgaat cagacgccct ttgctgattg ccatggccag tgatgaattc gcccggcggg
     4141 aggtaatccc gaacatcagt gccagctcat ccgccgtttg tgggccatga tgttcaatcg
     4201 cctcagtcag catttgcgct gtcactttcg gtaccggtga cactggttca ctttcaccag
     4261 tctgagtcag ccaccacatc gaccccttgt tatccgcttc accacggcgc ttcagtttcc
     4321 acagttcgtt gacagcatct tcgcggctga ttccaaggcg cgatgccact acctgtgaag
     4381 aggctttttt cagtgctttc agtgcgtcaa atacggtttc cattaatatt tcctccgaca
     4441 aaatcgtttc tcagattcaa ataaaaccag ctgccttccg gcgttcgtat tcctgtttca
     4501 gccgttcaat tggcgttggc ccttgcgggt gtttcgcccc ttccagttgt cgtcgcactg
     4561 gcggaacact catcccgtta ccaacatgct ttgcccattt cgtcagttgc cgttccgcaa
     4621 gtcgttttaa ctcaccttgc gtcatctggc gctcaatccc tctggtacgc atttcgaggc
     4681 agatgtggta cagcacaggc tgtggccacg ggtatttatc actcccgtcg tatcgccagg
     4741 attcattgcg ccagcgccgg tactcttcca tcacggcatc caccgtaaga ccaaatggat
     4801 ttgccccact ctccgaaatc agcgcaacaa actcagccag gtccgggggc cacgtttcac
     4861 ccgcccggca gcggtccatg cactgacggc acaccagacg gatttgctgt tcagtcatcg
     4921 caccaatctg ggcaatccag agcttcgaag gtgcggcccc gttcttctga gtccagcggt
     4981 tcgaatacac ctcccccatg agctcccaca gcttccaggc cgtttccgtt gctgataaat
     5041 ccgttgtcac gttcccactg ttcgcgtgca gcccggattt cctgaactgc ccgtgatgcc
     5101 gtgccacctg atgctgcatg gcttcccccc ttgctgactg gttttacctg tgccctgacg
     5161 tgctgcacgt ggcgggcaaa tttctgctcc cactgaacct gcgtgaaaac cttcccctcc
     5221 gccatccagt aatcccggaa tgcggcaagc tcagcaggtg taaattccgg ctcaggcaga
     5281 gccatacccc acactgctgc ccgttgtcga aaatccggcg acggctgcca gacagtagtc
     5341 atcgaaaatt tcccgatcgg ttcgctcagg ccgtccaggt attcaggttc ggctgtctgc
     5401 aacggcgcac cattcgactc actggtcgga atactctcgc gcgcgttatg tgtggggttt
     5461 aattctgtat ctgtatctgt atctttatct gtatctttat ctgtcgtgac ttgtcgtgac
     5521 agatgcgtga cacgtcgtga ctcatcgtga caatcagcat tatgtttccg cagcttttcc
     5581 cgctcccgct gcgctctctt gcgctctgcc ggggattttg ccgtttgcga aacgttacca
     5641 ttgtcctctt tcagcacctg acgtttttcc catccggaaa taaggtcacc atccagaacc
     5701 cgcccctgca ttgcatgcaa aattgaatca attacgtctt ccgtcacatc aagcgcactt
     5761 gctaaatctt ccgtcgtgac atcaatgtga ccacgtagtg acacgccgtg acatgtcgtg
     5821 acatttcgtg acgcgctcac cagaagatgg atatacactg ccatcactgt tgcgattggc
     5881 tgtcctgata ccctggcaat cgttcgccac ttggggtcat ttggcatgtc atgccacaat
     5941 ctgagccagg cattagccat actcacctct tctgataccg aactttaccc tcgaacatcc
     6001 ggaagaaatc cggcatgaat attgttggtc aatgcacgac aacagcatta ccaggctgac
     6061 ccccactgtt agtcagggtg ccccaggcga tcgccgctgc gacaaaatca tccacatctt
     6121 tcaccagccg atccctccgt tcgacgatct cacggtaata ttcagagctg tgactgcgca
     6181 tacgggccac cagcagaggc ggcattgcct tttcgatcgc cggtaacaga gcccgcattt
     6241 tttcaactgc atcaggggtg tctttctcta tccagcggaa aattttctgg gtattgcgag
     6301 ccagggcttc cggatggctg tcgtcgtaca gttcaggaaa cgtcataccc aactcaaaat
     6361 aagcctgggt tattccagct gccggaactt tttcgccatc aggacgcgcc caggcattca
     6421 tcgccatgcg gatgtgttca tgcttgattt tcatgaatcc ccccttggtt agaaggcgga
     6481 ttatgatcag aatcgggaat gacaaccgtc ggtatgtgta actcatattt gagcgccccg
     6541 gcagtgactg cctgaattag caacgcccat ttccacggaa cgtcttcccc ccacatgctg
     6601 actgtggttt ttgacgttcc tagagcggcg gctgttttaa caactccgcc aaaatagcct
     6661 aatacttctg attttttcat gagtcgctcc ataaaactga acgccaaaag tttaataatc
     6721 aaaaccaaag aaagtcaaga aacaaaacca tctgtgtttt aaaatcaaaa catgaacaag
     6781 caaacaatat ctgaacgcat aacccaacgt atgcatgcgc taaacttgaa aggcaaagac
     6841 cttgtcaatg ccactggcgc atcaaaaggc tccgtaagtc aatggatgaa cggtggagga
     6901 gcgccgtcct cgcgttacat aagttcactg gcaaagatat tgaaagtaaa cgaaaattgg
     6961 cttcttaatg gaggagagtt aaatacaggt gattcgcttg atctatcttt accgccgata
     7021 aaaacggttc cgctactatc acttcagcag gcagcaagct ggagtgatta tatgaaaaat
     7081 tcctcaataa cctcttgtgt gcagcttgtc ggagaaatcc cggccaatac ctttgctgtt
     7141 gttctagaga gtgacagtat gtcgacatct ggtggtggag tttccatccc aaatggttca
     7201 acagtttttg ttgatcccga tcgaatcgta caaccaggaa atattgtcct tgccttaccc
     7261 aaagggacca caacgcctgt cattcgcaaa ctggagatag aagggccgga tattctttta
     7321 gtccccacga atcctcgcta cccttcaatt atgctggatg atctatcttg catattggga
     7381 gtatgcttta aaattcaaca agatatttaa ccaacctcat ctatttgatt aactgtatgc
     7441 catcgtagtg atggcgtaac agctgcctgc ttaaaatgtt ttgataaaaa aacattgacc
     7501 ttaaatgttc gcttttctaa acttcattca ttccctcacc ccgccccaca gaatgcaggg
     7561 caatacttcg agttaccagg cagtggtcag gggttaagta gccagcccga ggcgtaagaa
     7621 catgacggca gggttcaact ttaataacta tgcagcaggt ttttgttccg ctaccccggc
     7681 gttaagggga aatgaggtca acatggatac gctcaatctt ggcaacaacg aatctctggt
     7741 atgtggcgtg ttccccaacc aggacggtac gttcaccgcg atgacgtata ccaaaagcaa
     7801 aacgtttaaa actgaagctg gcgcgcgtcg ctggttagcc agaaacactg actgatgagg
     7861 ttgacgatgg aatttaaaga tttacctcct tcaatccagg agattgcagc acacacactt
     7921 cgtcatcgtc tgaacgaact tgaattggaa tcggtaacga aaaaagacac tgataatatg
     7981 gctcgtaatg tgcgcgatgc gtttaccgga ttgtatttct gtgcgtctat aaataaacac
     8041 gactcagaga gtgtggcaaa taaaattgca gaaacgacag cgcaaaacat caatacgaaa
     8101 ccaacggaag aagaaattga tcagtttgct catgatgctg gtttaaaaaa caagaaagaa
     8161 aaatcgccat atgcggggaa catgtttgtt tatgacaatc tcatcagaat tcgtggcgaa
     8221 attccggcgg aatacctggc aagagtccat caggcattgc ttaaaaattt ggaaacagaa
     8281 ttatttgatg gcaacactaa cggtttcttc atggtatcag gccttgagaa agactgggat
     8341 gcagaaaaac gctggaatgt tgctacatgg ttattcagta acagagccgc tgcactggaa
     8401 gcttcggcat gtatttgcgg cctgttctta acagaccaca aatataatct ggatgtgtac
     8461 agttatattt acgctgaaca cggtccactc tggattgact ggtaattata aggaaacacc
     8521 agcagggccg cggcgaccaa cagaacgatt aaaatcaata atgccattat aaaggacatt
     8581 atttaattta tcgtcgaatg ctgattctgt gagcctcaac tctgaatgag tttttaataa
     8641 ccctgattgc ctgagttgat ttaccaggca ttcaatctgt ttttcaataa gcggatttct
     8701 ttttttgttt ggcattttat cctccattga ggttctgggt taaaaatgga gaccaacacg
     8761 ctgtcacgtg tggtcgtgcg ccggacacgg ataagaatcc ggtactgaca gtttactgaa
     8821 aggatatttc cctgaaaagt cagtgcataa cgcgaaagcg tacggcgaag ctctttccct
     8881 tagaaggctt gtcgttagat ttcttcgaac gtgcgcttcc ggttgtggca ctccgcgaaa
     8941 tggcgcggcg gtaagtatgg cggggttatt ccttccccat tgaggacacc gggttgtcag
     9001 gttgaccata cgcttaagtg acaaccccgc tgcaacgccc tctgttatca attttctggt
     9061 gacgtttggc ggtatcagtt ttactccgtg gctgctctgc cgcccttttt aaagtgaatt
     9121 ttgtgatgcg gtgaatgcgg ctaagcgcac gcggaacagt taaaaccaaa aacagtgtta
     9181 tgggtggatt ctctgtatcc ggcgttaatt gttaactggt taacgtcacc tggaggcacc
     9241 aggcactgca tcacaaaatt cattgttgag gacgcgataa tggaaacgtt attaccaaac
     9301 gttaatacgt ctgaaggttg ttttgatatt ggtgttctgc tcagtaaccg ggagtttact
     9361 gaagatgcca ttaacatgag gaaatatgag ccttatctgc tcaatgataa ttccatactt
     9421 tcccgaattg ctcttcttga acttggtatt ttcggagaac gtcaatgact tcagcatttg
     9481 cactgatgat gacggttttt cttataacgg gtgaatcaca gaatgtgatt accggaattt
     9541 atgccagtaa agaatcctgc ctccaggcaa gagacgagca aaaaatttct ggtgaatgcc
     9601 tcccggtaaa aaaagtatcg ctgtacctga ataacgaaac accggctgga taaccctcca
     9661 gccatattaa caccatacca acggattaaa aatgccagca atggcaggga tttgttcacc
     9721 cttaaatctg taatgaggtt tatcaatgag cactgataaa gaagaatttg cgctatattg
     9781 cgaagcaaaa aatgacaaag tcagaaaacg tctgggaatt aaaggtggtt tttactggac
     9841 tacagcaaaa aaattatctg ttgccatctc ccgctgcatt accgcaatgg atgacaacga
     9901 ttatgatgaa gacgacttta aaaaacccgt tcgcgtccat ttacccgttg tgaatgacct
     9961 tccacctgaa ggcgtgtttg ataccgaatt ctgcaaccga tacgaaaaag gcggggaaga
    10021 tggcattaca atggtattta tcgcgccctc tccctccgtg caggagaaac cagccagtac
    10081 tgacaatacc aacgtcaacg gcgaagacat gacggagatt gaggagaata tgctcctgcc
    10141 ggtttctggt caggagctgc ccattcgctg gcttgcgcaa cacggcagcg aaaaaccagt
    10201 aacgcacgtt gcacgggaag aacttcaggc attacatata gcacgggcgg aagaactgcc
    10261 ggctgttact gccctggcca tttctcacaa aacaaagctg ctcgacccgc tggagattcg
    10321 cgaccttcac aaactggtac gcgacactga caaagttttc cctaatcccg gtaattcaga
    10381 cctgggactg ataactgctt ttttcgaggc atacctggac gctgactaca ctgatcgggg
    10441 tctgctgaca aaagagtgga tgaaaggaaa tcgtgtttcg cgtatcaccc gtacggcttc
    10501 cggtgcaaat gccggtggcg ggaacaaaac cgatcgcaat ccgaatttag tacacacctt
    10561 cgatacgctg gatgtggaga ttgcagcagc cacacttccg atggatttta atatttatga
    10621 aattccgggc agcgtttatc gtcgcgcaaa agaaatcgtc ctgaaaagag aaagtccgtt
    10681 caaagaatgg tccgcagcac ttcgcgcaac cccgggtatt ctggactatt cccgcgccgc
    10741 tatttttgca cttatccgga gcgctcaccc tgagttttat cactacccgg gacgccttca
    10801 ggggtatatc aacgcctact taacggagac tgatcacgag aaccccagca aggaaactct
    10861 cactgctgca cgacatacac cggaaaaaga tatcctggaa gaagttaacc gcgaactgtc
    10921 tgctaagcag gaaacagaag aagaaaatga tgaagaaaaa ccgcaaccat cttgcgcaat
    10981 ggcagaatga taatcagaag aaatctggca aaagaccgtt ttacagtacc aaaaacacag
    11041 gcgggaacca atcgtgtgat tcaccttatt aagcccgcaa tcgacgctct ccggagtcag
    11101 atggcactaa cgagactgag caaagagcat atcatcgatg ttcacctcag agaattcggc
    11161 agaacagaga aacaaaaatg tacctttgtt tttcaacctg aagtgtcagc gaaagtaaaa
    11221 aattatggcg accattttac cgttgactca ataaggcaga tgtgggacgc agcggtaaag
    11281 cgtgccggaa tccgccatcg caaatcgtat cagtcgagac acacttatgc ctgctggtcg
    11341 ttaacagcag gagctaaccc ggcattcatt gcaaaccaga tgggccatgc agatgcgcaa
    11401 atggtgtttc aggtgtacgg gaaatggatg tcagaaaata ataatgcgca ggtaacgctg
    11461 ttaaatacac agttaagcga gtttgcccca accatgcccc ataacgaagc gatgaaaagt
    11521 taatttttta tttatcaatt agttagattg aat
//
LOCUS       DAZFBR010000132        11462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFBR010000132 DAZFBR010000000
VERSION     DAZFBR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.132"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            257..556
                     /gene="yoaC"
                     /locus_tag="ACHJZ2_004120"
     CDS             257..556
                     /gene="yoaC"
                     /locus_tag="ACHJZ2_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO4570130.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(676..855)
                     /gene="yoaH"
                     /locus_tag="ACHJZ2_004121"
     CDS             complement(676..855)
                     /gene="yoaH"
                     /locus_tag="ACHJZ2_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO4570131.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            929..2290
                     /gene="pabB"
                     /locus_tag="ACHJZ2_004122"
     CDS             929..2290
                     /gene="pabB"
                     /locus_tag="ACHJZ2_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO4570132.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2294..2872
                     /gene="nudL"
                     /locus_tag="ACHJZ2_004123"
     CDS             2294..2872
                     /gene="nudL"
                     /locus_tag="ACHJZ2_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO4570133.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3056..4420
                     /gene="sdaA"
                     /locus_tag="ACHJZ2_004124"
     CDS             3056..4420
                     /gene="sdaA"
                     /locus_tag="ACHJZ2_004124"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO4570134.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4551..6149
                     /gene="pdeD"
                     /locus_tag="ACHJZ2_004125"
     CDS             4551..6149
                     /gene="pdeD"
                     /locus_tag="ACHJZ2_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4570135.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6153..7709)
                     /gene="yoaE"
                     /locus_tag="ACHJZ2_004126"
     CDS             complement(6153..7709)
                     /gene="yoaE"
                     /locus_tag="ACHJZ2_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO4570136.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7697..7855)
                     /gene="yoaL"
                     /locus_tag="ACHJZ2_004127"
     CDS             complement(7697..7855)
                     /gene="yoaL"
                     /locus_tag="ACHJZ2_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO4570137.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8172..9143
                     /gene="manX"
                     /locus_tag="ACHJZ2_004128"
     CDS             8172..9143
                     /gene="manX"
                     /locus_tag="ACHJZ2_004128"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO4570138.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9206..10006
                     /gene="manY"
                     /locus_tag="ACHJZ2_004129"
     CDS             9206..10006
                     /gene="manY"
                     /locus_tag="ACHJZ2_004129"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO4570139.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10019..10870
                     /gene="manZ"
                     /locus_tag="ACHJZ2_004130"
     CDS             10019..10870
                     /gene="manZ"
                     /locus_tag="ACHJZ2_004130"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO4570140.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10925..11383
                     /gene="yobD"
                     /locus_tag="ACHJZ2_004131"
     CDS             10925..11383
                     /gene="yobD"
                     /locus_tag="ACHJZ2_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO4570141.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
BASE COUNT         2814 a         2888 c         2872 g         2888 t
ORIGIN      
        1 aaccatgccg ttgtgttcgc cctttgatta gcaactctgg ttttctttct tagctgaaca
       61 atctggttat agcgtgcgga ctgaccgtta attttctcca tcttactgga taacacacca
      121 tgagctgcta cctttaaaac acctgaatat caacaggtta actcgtgcca tatcgttata
      181 atccctttga tattagatgc aaattaaggt catatatagc cttatcgata acatggttaa
      241 ttttaaggat aagaatatgc ctgctgtaat agataaagcc ctggatttca ttggtgccat
      301 ggatgtatca gcgccaacac caagttcgat gaatgaaagc acggcgaagg gaatctttaa
      361 atatttaaaa gaactgggag tacccgcgag tgccgctgat attacggcgc gagctgacca
      421 ggaaggctgg aaccctgggt tcacggaaaa aatggttgga tgggcaaaaa aaatggagac
      481 aggtgaacgc tctgtgatta aaaatcctga gtacttttca acatatatgc aggaagaact
      541 aaaagcactg gtctgagtta aatttatatc agcataaatg ggtcaggacg cttttaatca
      601 catataaaaa gcgtcctttt tctcacaatc aaataatcat cagcttctat aacgaaatcg
      661 atacggatta gtgtgttact cgtcttcatc ctcaaaacgc gccacgattc gctcgccgga
      721 gtggttggca cgcagctctt ctgccaccag cgcgattgcc tggccgctgc tcatcccttg
      781 tgccatcagt tcctggatcc gctcgacagc tttttgctgc tgttcatggg tgagtgaagg
      841 taaacctgca aacattgtta actcctgcta aattgttggc gctaattatt tcatgctacc
      901 cggcacatag ccagtagagt caggactgat gaagacgtta tctcccgctg tgattacttt
      961 accctggcgt caggacgccg ctgaatttta tttctcccgc ttaagccacc tgccgtgggc
     1021 gatgctttta cactccggct atgccgatca tccgtatagc cgctttgata ttgtggtcgc
     1081 cgatccgatt tgcactttaa ccactttagg taaagaaacc gttgttagtg aaagcgaaaa
     1141 acgcacaacg accactgatg acccgctaca ggtgctccag caggtgctgg atcgcgcaga
     1201 cattcgccca acgcataacg aagatttgcc atttcagggc ggcgcactgg ggttgtttgg
     1261 ctacgatctg ggccgccgtt ttgagtcact gccagaaatt gcggaacaag atatcgttct
     1321 gccggatatg gcagtgggta tctacgattg ggcgctcatt gtcgaccacc agcgtcatac
     1381 agtttctttg ctgagtcata atgatgtcaa tgcccgtcgg gcctggctgg aaagccagca
     1441 attctcgccg caggaagatt tcacgctcac ttccgactgg caatccaata tgacccgcga
     1501 gcagtacggc gaaaaatttc gccgggtaca ggaatatctg cacagcggtg attgctatca
     1561 ggtgaatctc gcccagcgtt ttcatgcgac ctattctggc gatgaatggc aggcattcct
     1621 tcagcttaat caggccaacc gcgcgccatt tagcgctttt ttacgtcttg aacagggtgc
     1681 aattttaagc ctttcgccag agcggtttat tctttgtgat aatagtgaaa tccagacccg
     1741 cccgattaaa ggcacgctac cacgcctgcc cgctcctcag gaagatagca aacaagcaga
     1801 aaaactggcg aactcagcga aagatcgtgc cgaaaatctg atgattgtcg atttaatgcg
     1861 taatgatatc ggtcgtgttg ccgtagcagg ttcggtaaaa gtaccagagc tgttcgtggt
     1921 ggaacccttc cctgccgtgc atcatctggt cagcaccata acggcgcaac taccagaaca
     1981 gttacacgcc agcgatctgc tgcgcgcagc ttttcctggt ggctcaataa ccggggctcc
     2041 gaaagtacgg gctatggaaa ttatcgacga actggaaccg cagcgacgta atgcctggtg
     2101 cggcagcatt ggctatttga gcttttgcgg caacatggat accagcatta ctatccgcac
     2161 gctgactgcc attaacggac aaatatactg ctctgcggga ggtggaattg tcgccgatag
     2221 ccaggaagaa gcggaatatc aggaaacttt tgataaagtt aataagatat tacgccaact
     2281 ggagaagtaa gacgtggaat accgtagcct gacgcttgat gattttttat cgcgctttca
     2341 acttttgcgc ccgcaaatta accgggaaac cctaaatcat cgtcaggctg ccgtgttaat
     2401 ccccatcgtc cgtcgaccgc aaccggggtt gttgctgact cagcgttcaa ttcatctgcg
     2461 taaacacgct ggacaagtgg cattccctgg aggtgcagtc gatgacacgg acgcatcagt
     2521 tatcgccgcc gcgctgcgcg aagctgaaga agaggttgct ataccgcctt ccgccgttga
     2581 agttatcggc gtgctgccgc ccgtcgatag cgtaactggc taccaggtaa ccccagtggt
     2641 cggcattatc ccgcccgatc tgccgtatcg cgccagtgaa gatgaagttt ccgctgtgtt
     2701 tgaaatgccg ctcgcccagg cattacatct gggtcgttat caccctttag atatctaccg
     2761 ccgcggcgat tcacatcggg tatggctatc ctggtacgaa cagtattttg tatggggaat
     2821 gaccgcaggc ataattcgtg agctggcgct gcaaattggt gtgaaaccct gactatactt
     2881 atctttacat ctacaaaaca ctacttgaga caatcatcgc aatattagtt aaatcgcggt
     2941 ttttgattag tttaattcat gtgaatagtt aagccagtcg ccgcgttccc tcttacacta
     3001 tgcgctgtta ttagttcgtt actggaagtc cagtcacctt gtcaggagta ttatcgtgat
     3061 tagtctattc gacatgttta aggtggggat tggtccctca tcttcccata ccgtagggcc
     3121 tatgaaggcg ggtaaacagt tcgtcgatga tctggtcgaa aaaggcttac tggatagcgt
     3181 tactcgcgtt gccgtggacg tttatggttc actgtcgctg acgggtaaag gccaccacac
     3241 cgatatcgcc attattatgg gtcttgcagg taacgaacct gccaccgtgg atatcgacag
     3301 tattcccggt tttattcgcg acgtagaaga gcgcgaacgt ctgctgctgg cacagggacg
     3361 gcatgaagtg gatttcccgc gcgacaacgg gatgcgtttt cataacggca acctgccgct
     3421 gcatgaaaac ggtatgcaaa tccacgccta taacggcgat gaagtcgtct acagcaaaac
     3481 ttattattcc atcggcggcg gttttatcgt cgatgaagaa cactttggtc aggatactgc
     3541 caacgaagtg agcgtgccgt atccgttcaa atctgccacc gaactgctcg cgtactgtaa
     3601 tgaaaccggc tattcgctgt ctggtctcgc tatgcagaac gaactggcgc tgcacagcaa
     3661 gaaagagatc gacgagtatt tcgcgcatgt ctggcaaacc atgcaggcat gtatcgatcg
     3721 cgggatgaac accgaaggtg tactgccagg cccgctgcgc gtgccacgtc gtgcgtctgc
     3781 cctgcgccgg atgctggttt ccagcgataa actgtctaac gatccgatga atgtcattga
     3841 ctgggtaaac atgtttgcgc tggcagttaa cgaagaaaac gccgccggtg gtcgtgtggt
     3901 aactgcgcca accaacggtg cctgcggtat cgttccggca gtgctggctt actatgacca
     3961 ctttattgaa tcggtcagcc cggacatcta tacccgttac tttatggcag cgggcgcgat
     4021 tggtgcattg tataaaatga acgcctctat ttccggtgcg gaagttggtt gccagggcga
     4081 agtgggtgtt gcctgttcaa tggctgctgc gggtcttgca gaactgctgg gcggtagccc
     4141 ggaacaggtt tgcgtggcgg cggaaattgg catggaacac aaccttggtt taacctgcga
     4201 cccggttgca gggcaggttc aggtgccgtg cattgagcgt aatgccattg cctctgtgaa
     4261 ggcgattaac gccgcacgga tggctctgcg ccgcaccagt gcaccgcgcg tctcgctgga
     4321 taaggtcatc gaaacgatgt acgaaaccgg taaggacatg aacgccaaat accgcgaaac
     4381 ctcacgcggt ggtctggcaa tcaaagtcca gtgtgactaa tacttcttac tcgcccatct
     4441 gcaacggatg ggcgaattta tccccgcttt ctcgtctgct gtaatattcc ccactacact
     4501 tccactgttg cgtcaggcgt ttgtcgccat acgcttacag agtggcccgc atgcaaaaag
     4561 cacaacggat cattaaaacc tatcgccgta atcgaatgat tgtttgtacg atttgcgcac
     4621 tcgttacgct cgcttcgacc ctgagcgtgc gatttatttc acagcgtaac ttaaatcaac
     4681 aacgggtagt acaattcgcc aatcacgctg tagaggaatt agataaagta ctgcttcccc
     4741 tacaggcagg tagcgaagtc ttgcttccgc tgattggtct gccctgctct gtcgcccatt
     4801 tgccattacg taaacaggcg gcaaaactcc aaactgtgcg atccattggc ctggtgcaag
     4861 acggcacact ttattgctcc agcatttttg gttatcgcaa tgtgcccgtc gtggacattc
     4921 tggctgaact tcctgcaccg caaccacttt tacgcctgac gaccgaccgt gtcctgatta
     4981 aaggcagtcc ggttttgatt caatggacgc ctgcagcgag cagtagcaat gctggggtca
     5041 tggagatgat taacatcgac ttactgacgg caatgctgct tgagccacaa ctgccgcaaa
     5101 tcagtagcgc cagcctgacg gtggacaaac ggcatttgct ctatggtaat gggctggtag
     5161 attcccttcc acaacctgaa gacaatgaaa actatcaggt ttcttcgcaa cgcttttctt
     5221 ttaccattaa cgttaatggt ccgggggctc cggcgctggc atggcactat cttccaacac
     5281 aattaccgct ggcggtactg ctaagtttac tggtgggcta catcgcctgg ctggcgaccg
     5341 cttaccgaat gagcttttcc cgcgaaatca atctgggcct ggcgcaacat gagttcgaat
     5401 tgttctgtca gcctttgctt aatgcgcgca gccagcaatg tattggtgta gagattttgc
     5461 tgcgctggaa caatccgcgt cagggctgga tttcaccgga tgtgtttatt cctatcgcgg
     5521 aagaacatca tttaattgtg ccactgaccc gctatgtgat ggcagaaacc attcgtcagc
     5581 gccatgtttt cccgatgagt agtcagtttc atgttggcat taacgtcgca cccagccatt
     5641 ttcgccgtgg tgtgctgata aaagatctca atcagtactg gtttagcgct cacccgattc
     5701 agcaactgat cctcgaaatc accgaacgcg atgccttact ggatgttgat tatcggattg
     5761 cccgcgagct acatcgtaaa aacgtcaaac tggcgattga tgacttcggc accggcaaca
     5821 gttcattttc ctggcttgaa acattacgtc ctgacgtgct gaaaattgat aagtcattta
     5881 ccgcagctat aggttctgac gcggttaact cgacggtgac cgatatcatc atcgcgctgg
     5941 ggcaaagact gaatattgaa ctggtggcgg agggcgtgga aacgcaagaa caggcgaagt
     6001 atttgcgccg tcatggcgtg catattttgc aagggtattt gtacgcacag ccgatgccgc
     6061 tacgtgattt tcccaaatgg ctggcgggca gccaaccgcc gcccgcccgg cataatggac
     6121 atatcacgcc cattatgccg ttacgttaac gcttactcat cttcatcgtg cgccggttgc
     6181 tctttaacaa tgcgaaccag atcaacacga taatcattgg cttcaatgat ggtgatatgc
     6241 agtggcccta catcaatcac atcgcccaca cggggaatgt gaccatttgc cgagatcacg
     6301 aggcccgcga ccgtcgcgat atcatcgtca tcggcaaggt gctcaacatc aagcgcctgc
     6361 tgcaaggcat gcaaatctgt accgcctttt accagccagc cgtcaccatc agtaatgatt
     6421 tccggcgttt cgtcagcgtc cgggaattca cccgcaatgg cttccagcac atccagcggc
     6481 gtgaccaagc cttgtaccac accaaactcg ttggtcacga taacaaagct cccgcgagca
     6541 cgacgcagca cgcccagcag gttgatcgga tcgagggttt cagggacgat aatcgccgga
     6601 gacgccgaag caatcgccgc cacatcaacg ccctcttcca gcgccaccag cagttcttta
     6661 gcacgtacga ttccgatgat ttcatccagt tcaccgcgac ataccgggaa caggctgtgc
     6721 ggtgaggaga gcagctgctc gcggatttca tcgaccccga gattagcgtc aacccagctt
     6781 atttcaccgc gcggcgtcat gatcccgcgc agagaacgcg acgccagcgt cagtacgccg
     6841 ttaatcatgt aacgttcttc ttcggcaaat gcaccttccg ggatcggcat cggcatcggg
     6901 ttatcggcat cgtgctgaac attggcctga cgtttcccgc ccatcaaacg caggatggca
     6961 tcggcagtac gcgctcgcag cggcaaagtc gactggtgac gaataaagtt gcgacgcgca
     7021 atctggttaa acacttcgat gatgatcgag aagccaatcg cggcatacag gtaacctttc
     7081 ggaatgtgga aaccgaaacc ttctgccacc agactcagac caatcattaa caggaagctc
     7141 agacagagca ccaccaccgt ggggtgctgg ttaacgaatc gcgtcagcgg tttggatgcc
     7201 agcaacataa ccgccatcgc aatcactacc gccgccatca tcaccggcag atggttaacc
     7261 atccctactg cagtaattac cgcatccaac gagaagacgg cgtcaaggat gacaatctgt
     7321 gtgacgacca cccagaaact ggcgtagcct ttaccgtggc cggaatcatg atcgcggttt
     7381 tccagccgtt catgcagttc ggttgttgct ttgaacagca agaatatccc cccgaacaac
     7441 ataatcaggt cgcgtccgga gaaggagaaa tccatgacgg taaatagcgg tttggtcagc
     7501 gtgaccatcc atgaaatcag cgacagcagc cccagacgca taatcagcgc cagtgataac
     7561 cccagcaaac gcgctttatc gcgttgtttt ggcggcagtt tgtcagcaag aatggcgatg
     7621 aagaccaggt tatcgatacc cagcacaatt tcgagaacaa caagcgtgag tagccccgcc
     7681 caaattgagg ggtccattaa gaattccatg acaagctcct gcttaaggaa tagctattcg
     7741 acgccagaaa taatgcaggc gtaacgacaa aatgcaaacg aaaggtgcgg catagagtgc
     7801 cagaaaggca ggcgttaaaa ggcctgatgc tgaaatgacg tcggtgacga tccatactgc
     7861 gggctactgc cctatactcc atgattgtta aacgggagtt aaacatatca gagacgcctc
     7921 tgatttggca aagatttacc ttcctttgca aacgaatgtg acaaggatat tttacctttc
     7981 gaaatttctg ctaatcgaaa gttaaattac ggatcttcat cacataaaat aattttttcg
     8041 atatctaaaa taaatcgcga aacgcagggg tttttggttg tagcccttat ctgaatcgat
     8101 tcgattgtgg acgacgattc aaaaatacat ctggcacgtt gaggtgttaa cgataataaa
     8161 ggaggtagca agtgaccatt gctattgtta taggcacaca tggttgggct gcagagcagt
     8221 tgcttaaaac ggcagaaatg ctgttaggcg agcaggaaaa cgtcggctgg atcgatttcg
     8281 ttccaggtga aaatgccgaa acgctgattg aaaagtacaa cgctcagttg gcaaaactcg
     8341 acaccactaa aggcgtgctg tttctcgttg atacatgggg aggcagcccg ttcaatgctg
     8401 ccagccgcat tgtcgtcgac aaagagcatt atgaagtcat tgcaggcgtt aacattccaa
     8461 tgctcgtgga aacgttaatg gctcgtgatg atgacccaag ctttgatgaa ctggttgcgc
     8521 tggcagtaga aacaggccgt gaaggcgtga aagcactgaa agccaaaccg gttgaaaaag
     8581 ccgcgccagc acccgctgcc gcagcaccca aagcggctcc aactccggca aaaccaatgg
     8641 ggccaaacga ctacatggtt attggccttg cgcgtatcga cgaccgtctg attcacggtc
     8701 aggtcgccac ccgctggacc aaagaaacca atgtctcccg tattattgtt gttagtgatg
     8761 aagtggctgc ggatactgtt cgtaagacac tgctcaccca ggttgcacct ccgggcgtaa
     8821 cagcacacgt agttgatgtt gccaaaatga ttcgcgtcta caacaacccg aaatatgctg
     8881 gcgaacgtgt aatgctgtta tttaccaacc caacagatgt agagcgtctc gttgaaggcg
     8941 gcgtgaaaat cacctctgtt aacgtcggtg gtatggcatt ccgtcagggt aaaacccaag
     9001 tgaataacgc ggtttcggtt gatgaaaaag atatcgaggc gttcaaaaaa ctgaatgcac
     9061 gcggtattga gctggaagtc cgtaaggttt ccaccgatcc gaaactgaaa atgatggatc
     9121 tgatcagcaa aatcgataag taacgtattg tgttgattat cagtcagttt tcacacttta
     9181 gtcatacgta aacaggagaa gtacaatgga gattaccact cttcaaattg tgctggtatt
     9241 tatcgtagcc tgtatcgcag gtatgggatc aatcctcgat gaatttcagt ttcaccgtcc
     9301 gctaatcgcg tgtaccctgg tgggtatcgt tcttggggat atgaaaaccg gtattattat
     9361 cggtggtacg ctggaaatga tcgcgctggg ctggatgaac atcggtgctg cagttgcgcc
     9421 tgacgccgct ctggcatcta tcatttctac cattctggtt atcgcaggtc atcagagcat
     9481 tggtgcaggt attgcactgg caatccctct ggccgctgcg ggccaggtac tgaccatcat
     9541 cgttcgtact attaccgttg ctttccagca cgctgcggat aaggctgctg ataacggcaa
     9601 cctgacagcg atttcctgga tccacgtttc ttctctgttc ctgcaagcaa tgcgtgtggc
     9661 tattccggcc gtcatcgttg cgctgtctgt aggtaccagc gaagtacaga acatgctgaa
     9721 tgcgattccg gaagtggtga ccaatggtct gaatatcgcc ggtggcatga tcgtggtggt
     9781 tggttatgcg atggttatca acatgatgcg tgctggctac ctgatgccgt tcttctacct
     9841 cggcttcgta accgcagcat tcaccaactt taacctggtt gctctgggtg tgattggtac
     9901 tgttatggca gtgctctaca tccaacttag cccgaaatac aaccgcgtag ccggtgcgcc
     9961 tgctcaggca gctggtaaca acgatctcga taacgaactg gactaacagg tgagcgaaat
    10021 ggttgataca actcaaacta ccaccgagaa aaaactcact caaagtgata ttcgtggcgt
    10081 cttcctgcgt tctaacctct tccagggttc atggaacttc gaacgtatgc aggcactggg
    10141 tttctgcttc tctatggtac cggcaattcg tcgcctctac cctgagaaca acgaagctcg
    10201 taaacaagct attcgccgtc acctggagtt ctttaacacc cagccgttcg tggctgcgcc
    10261 gattctcggc gtaaccctgg cgctggaaga acagcgtgct aatggcgcag agatcgacga
    10321 cggtgctatc aacggtatca aagtcggttt gatggggccg ctggctggtg taggcgaccc
    10381 gatcttctgg ggaaccgtac gtccggtatt tgcagcactg ggtgccggta tcgcgatgag
    10441 cggcagcctg ttaggtccgc tgctgttctt catcctgttt aacctggtgc gtctggcaac
    10501 ccgttactac ggcgtagcgt atggttactc caaaggtatc gatatcgtta aagatatggg
    10561 tggtggcttc ctgcaaaaac tgacggaagg ggcgtctatc ctcggcctgt ttgtcatggg
    10621 ggcattggtt aacaagtgga cacatgtcaa catcccgctg gttgtctctc gcattactga
    10681 ccagacgggc aaagaacacg ttactactgt ccagactatt ctggaccagt taatgccagg
    10741 cctggtacca ctgctgctga cctttgcttg tatgtggcta ctgcgcaaaa aagttaaccc
    10801 gctgtggatc atcgttggct tcttcgtcat cggtatcgct ggttacgctt gcggcctgct
    10861 gggactgtaa gactgttgta cactaccggg gccttttggc cccgtttttt tatctggagg
    10921 attaatgaca atcacggacc tggtactgat tcttttcatc gccgcactcc tggccttcgc
    10981 gatctacgat cagttcatca tgccccgccg taacggcccc accctgctgg caattccttt
    11041 gctccggcgt ggtcgcatcg atagcgttat cttcgtcgga ttgattgtca ttcttatcta
    11101 taacaacgtc acgaatcatg gtgcgttaat aacgacatgg ttattaagcg cactggctct
    11161 gatgggtttt tatatattct ggatccgcgt tccgaagatc atctttaaac aaaaaggttt
    11221 tttcttcgcc aatgtctgga ttgaatatag ccgaatcaaa gcgatgaact tgtcggaaga
    11281 tggcgtgttg gtgatgcaat tagaacagcg tcggctgtta atccgcgttc gaaatatcga
    11341 cgatctggaa agaatttata aacttctcgt ttcaactcaa tgagttatta atttagccaa
    11401 agctatgttt agtgtatttt taataatcag acatagctta ggctatatta cctcttccct
    11461 ta
//
LOCUS       DAZFBR010000133        11399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFBR010000133 DAZFBR010000000
VERSION     DAZFBR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.133"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(134..310)
                     /locus_tag="ACHJZ2_004132"
     CDS             complement(134..310)
                     /locus_tag="ACHJZ2_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570142.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
     gene            613..1239
                     /locus_tag="ACHJZ2_004133"
     CDS             613..1239
                     /locus_tag="ACHJZ2_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4570143.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKNELLIVKSE"
     gene            1590..1757
                     /locus_tag="ACHJZ2_004134"
     CDS             1590..1757
                     /locus_tag="ACHJZ2_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4570144.1"
                     /translation="MLHSAMHSLMNSQARDTRDRSQNDDNVLEISEPCIRSTFERLYQ
                     NMNEVFGLGDY"
     gene            complement(1837..3084)
                     /locus_tag="ACHJZ2_004135"
     CDS             complement(1837..3084)
                     /locus_tag="ACHJZ2_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO4570145.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3156..4070)
                     /locus_tag="ACHJZ2_004136"
     CDS             complement(3156..4070)
                     /locus_tag="ACHJZ2_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO4570146.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            4286..5719
                     /gene="cscA"
                     /locus_tag="ACHJZ2_004137"
     CDS             4286..5719
                     /gene="cscA"
                     /locus_tag="ACHJZ2_004137"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO4570147.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHIAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(5727..6722)
                     /locus_tag="ACHJZ2_004138"
     CDS             complement(5727..6722)
                     /locus_tag="ACHJZ2_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4570148.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            6972..7190
                     /locus_tag="ACHJZ2_004139"
                     /pseudo
     CDS             6972..7190
                     /locus_tag="ACHJZ2_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7208..8536
                     /gene="dsdA"
                     /locus_tag="ACHJZ2_004140"
     CDS             7208..8536
                     /gene="dsdA"
                     /locus_tag="ACHJZ2_004140"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO4570149.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCSVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(8644..10182)
                     /gene="emrY"
                     /locus_tag="ACHJZ2_004141"
     CDS             complement(8644..10182)
                     /gene="emrY"
                     /locus_tag="ACHJZ2_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO4570150.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     SINLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVIFLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(10182..11345)
                     /gene="emrK"
                     /locus_tag="ACHJZ2_004142"
     CDS             complement(10182..11345)
                     /gene="emrK"
                     /locus_tag="ACHJZ2_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO4570151.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3272 a         2718 c         2571 g         2838 t
ORIGIN      
        1 aaaattgtga tacttatgac agatttgagt gacattttat caatctactg attttgatgg
       61 tgaaatttat tagaaggtat gcgtagtgct acgggagaaa tgagaaacgc gcccagcaat
      121 ggcgcatcct ttcttaagca agttggctag tatctatagc atccatccaa tcaggagcaa
      181 ttcccaaacg ttccgcgaac tcttcattgg tcacgtcttc cattgagaga ctagccagac
      241 aatcgttaat ttcagaatag acgggggaaa gtaataggta ttcccgcaac tctttcaggt
      301 ctttttccat cttaacactc cttgttaata gaccgtcgaa caatggtaca gatttgtacc
      361 attccaagta ctggcaagca accccaccag cacaacaaat tatctcatac ggtaaaaacg
      421 gtgtcgggct gtttctttcg gctagctcaa tatatcacct taaacaccag ccgttatcat
      481 tgtctcaatt gggtataccg taatgacaag gtgatacaaa gtctcaatag ggttgtatag
      541 gaacattttg actacacaaa ctgcattatg tatcatttgc acttatattc acaacccttt
      601 tgagacaatg caatgaacgt tagaatttac tgccgagcat caactgaggg gcaacacgct
      661 gatcgagcac ttaccagcct acgtgaattt tcaaaatcaa aaggctggca gattgcagga
      721 gagtatatag aaaatgctag cggggcgaag cttgagcgcg tagagcttat gcggctattg
      781 tctgaagccc agtccggcga tctgttgcta gtggaagcta tagatcgtct gagtcgccta
      841 gaacatagtg catgggtcga actgaaagat acgctcaacc gaaaagggtt aatcatcgta
      901 tcaatggatt taccaacgag ctggcagatg gtcgagatgg caggcaatga tctaaccagc
      961 ggcatcctga gagccgtaaa tgccatgttg attgatatcc ttgccacaat ggcacggcag
     1021 gattacgaaa cacgccgtaa acgccaacag caaggcattg aacgagctaa aagcgaaggc
     1081 atatacattg ggagggcgaa aaatcaggaa gcccgagaga tcgttagaga gatgttggag
     1141 caaggagtaa aacctgaact catcatgaag gcagcaggaa tcagccgagc gacttactac
     1201 cggataaaaa acgagctatt gatcgtaaaa tcagaataaa atttgaaagg tcgtcaggct
     1261 tgtttcatca ggtaaaaacc agcatttctg atcacaacgc ttgggggcac ctattggggc
     1321 actcaccaaa atcgaaagag aaataaacta caattcaaat tgttagctaa tttttcgatt
     1381 cctgcaggga caccagatac ccttcaaacc aaatctacct tcatcccgta aacgatgggt
     1441 ttggcagcac acttgcccta tatctactca tttttactgc aacaggttga aatctcagca
     1501 ctgtcagaaa gcgctgatga ctaaacagcc cagggccggg cgatgtaacc atcacacaga
     1561 atcctgatag cgaaatacgg cgtgactcga tacttcactc cgcaatgcat tccttgatga
     1621 attcgcaggc ccgtgataca cgggacaggt cgcagaatga cgacaatgtc ctggaaatca
     1681 gcgaaccgtg tatccggagt acatttgagc gactgtacca gaacatgaat gaggtgtttg
     1741 gattaggcga ttattagcag ggctaagcat tttagtatta ttattttccg gttgaaggat
     1801 atggagatat cgacaacaac cggaaaaagt ttacgtctat attgctgaag gtacaggcgt
     1861 ttccataact atttgctcgc gttttttact caggaagaaa atgccaaata gcaacatcag
     1921 gcagacaata cccgaaattg cgaagaaaac tgtctggtag cctgcgtggt caaagagtat
     1981 cccagtcggc gttgaaagca gcacaatccc aagcgaactg gcaatttgaa aaccaatcag
     2041 aaagatcgtc gacgacaggc gcttatcaaa gtttgccacg ctgtatttga agacggatat
     2101 gacacaaagt ggaacctcaa tggcatgtaa cagcttcact aatgaaataa tccaggggtt
     2161 aacgaacagc gcgcaggaaa ggatacgcaa cgccataatc acaacaccga taagtaatgc
     2221 attttttggc cctacccgat tcacaaagaa cggaataatc gccatgcaca gcgcttcgag
     2281 taccacctgg aatgagttga gataaccata caggcgcgtt cctacatcgt gtgattcgaa
     2341 taaacctgca taaaagacag gaaaaagttg ttgatcaaaa atgttataga aagaccacgt
     2401 cccaacaata aatatgacga aaacccagaa gtttcgatcc ttgaaaactg cgataaaatc
     2461 ctcttttttt acccctcccg catccgccgc tacgcactgg tgatccttat ctttaaaaca
     2521 catgttgatc atcataaata cagcgccaaa tagcgagacc aaccagaagt tgatatgggg
     2581 actgatacta aaaaatatgc cggcaaagaa cgcgccaata gcatagccaa aagatcccca
     2641 ggcgcgcgct gttccatatt cgaaatgaaa atttcgcgcc attttttcgg tgaagctgtc
     2701 aagcaaaccg catcccgcca gataccccag gccaaaaaag agcgccccca gaattagacc
     2761 tacagaaaaa ttgctttgca gtaacggttc ataaacgtaa atcataaacg gtccggtcaa
     2821 gaccaggatg aaactcatac accagatgag cggtttcttc agaccgagtt tatcctgaac
     2881 gatgccgtag aacatcataa atagaatgct ggtaaactgg ttgaccgaat aaagtgtacc
     2941 taattccgtc cctgtcaacc ctagatgtcc tttcagccaa atagcgtata acgaccacca
     3001 cagcgaccag gaaataaaaa agagaaatga gtaactggat gcaaaacgat agtacgcatt
     3061 tctgaatgga atattcagtg ccataattac ctgcctgtcg ttaaaaaatt cacgtcctat
     3121 ttagagataa gagcgacttc gccgtttact tctcactatt ccagttcttg tcgacatggc
     3181 agcgctgtca ttgccccttt cgccgttact gcaagcgctc cgcaacgttg agcgagatcg
     3241 ataattcgtc gcatttctct ctcatctgta gataatcccg tagaggacag acctgtgagt
     3301 aacccggcaa cgaacgcatc tcccgccccc gtgctatcga cacaatccac agacattcca
     3361 gcaaaatggt gaacttgtcc tcgataacag accaccaccc cttctgcacc tttagtcacc
     3421 aacagcatgg cgatctcata ctcttttgcc agggcgcata tatcccgatc gttctgtgtt
     3481 tttccactga taagtcgcca ttcttcttcc gagagcttga cgacatccgc cagttgtagc
     3541 gcctgccgca aacacaagcg gagcaaatgc tcgtcttgcc atagatcttc acgaatattg
     3601 ggatcgaagc tgacaaaacc tccggcatgc cggatctccg tcatcgcagt aaatgcgctg
     3661 gtacgcgaag gctcggcaga caacgcaatt gaacagagat gtaaccattc gccatgtcgc
     3721 cagcagggca agtctgtcgt ctctaaaaaa agatcggcac tggggcggac cataaacgta
     3781 aatgaacgtt ccccttgatc gttcagatcg acaagcaccg tggatgtccg gtgccattca
     3841 tcttgcttca gatacgtgat atcgactccc tcagttagca gcgttctttg cattaatgca
     3901 ccaaaaggat catcccccac ccgacctata aacccacttg ttccgcctaa tctggcgatt
     3961 cccaccgcaa cgttagctgg cgcgccgcca ggacaaggca gtagccgccc gtctgattct
     4021 ggcaagagat ctacgaccgc atcccctaaa acccatactt tggctgacat ttttttccct
     4081 taaattcatc tgacttacgc atagtgataa acctcttttt cgcaaaatcg tcatggattt
     4141 actaaaacat gcatattcga tcacaaaatg tcatagttaa cgttaacatt tgtgatattc
     4201 atcgcattta tgaaagtaag ggactttatt tttataaaag ttaacgttaa caattcacca
     4261 aatttgctta accaggatga taaaaatgac gcaatctcga ttgcatgcgg cgcaaaacgc
     4321 actagcaaaa cttcacgagc gccgaggtaa cactttctat ccccattttc acatcgcgcc
     4381 tcctgccggg tggatgaacg atccaaacgg cctgatctgg tttaacgatc gttatcacgc
     4441 gttttatcaa catcacccaa tgagtgaaca ctgggggcca atgcactggg gacatgccac
     4501 cagcgacgat atgatccact ggcagcatga gcctattgcg ctagcgccag gagacgagaa
     4561 tgacaaagat gggtgttttt caggtagtgc tgtcgatgac aatggtgtcc tctcacttat
     4621 ctacaccgga cacgtctggc tcgatggtgc aggtaatgac gatgcaattc gcgaagtaca
     4681 atgtctggct accagtcggg atggtattca tttcgagaaa cagggtgtga tcctcactcc
     4741 accagaagga atcatgcact tccgcgatcc taaagtgtgg cgtgaagccg acacatggtg
     4801 gatggtagtc ggggcgaaag acccaggcaa cacggggcag atcctgcttt atcgcggcag
     4861 ttcattacgt gaatggactt tcgatcgcgt actggcccac gctgatgcgg gtgaaagcta
     4921 tatgtgggaa tgtccggact ttttcagcct tggcgatcag cattatctga tgttttcccc
     4981 gcagggaatg aatgccgagg gatacagtta ccgaaatcgc tttcaaagtg gcgtaatacc
     5041 cggaatgtgg tcgccaggac gactttttgc acaatccggg cattttactg aacttgataa
     5101 cgggcatgac ttttatgcac cacaaagctt tgtagcgaag gatggtcggc gtattgttat
     5161 cggctggatg gatatgtggg aatcgccaat gccctcaaaa cgtgaaggct gggcaggctg
     5221 catgacgctg gcgcgcgagc tatcagagag caatggcaaa cttctacaac gcccggtaca
     5281 cgaagctgag tcgttacgcc agcagcatca atctatctct ccccgcacaa tcagcaataa
     5341 atatgttttg caggaaaacg cgcaagcagt tgagattcag ttgcagtggg cgctgaagaa
     5401 cagtgatgcc gaacattacg gattacagct cggcactgga atgcggctgt atattgataa
     5461 ccaatctgag cgacttgttt tgtggcggta ttacccacac gagaatttag acggctaccg
     5521 tagtattccc ctcccgcagc gtgacacgct cgccctaagg atatttatcg atacatcatc
     5581 cgtggaagta tttattaacg acggggaagc ggtgatgagt agtcgaatct atccgcagcc
     5641 agaagaacgg gaactgtcgc tttatgcctc ccacggagtg gctgtggtgc aacatggagc
     5701 actctggcaa ctgggttaac ataatatcag gtggaacaac ggatcaacag cgggcaaggg
     5761 atccgcgtca cacttccccc ttcacgacct tcaataatat gcaatgcagc ttcccgcccg
     5821 ataatgtcat gtggaagctg aattgtggtc agcggcggta aaaacagatg cccgacgcca
     5881 accagattat caaagcccat tacggcgaca tcctgcggga tacgtacccc cttagccagg
     5941 agaacctgat aagccacaaa ggctgcgcga tcgttaccac atatcagaac atcaaaatct
     6001 ggtttgcccg atttgaagtg gtcattgagt aaacttgcga gatcggtgta gtgatcatca
     6061 cctgttgcca tgtgaaattg tttcacctca gccagatctc gtccagcatc acgccaggcc
     6121 tgctcaaatc cctgccgacg ataccctgtt gccaacgcac tttccggtag ccagaagcat
     6181 aacggttgac gatagcccgc cgcgagcaaa tgctgtgttg attcatattg tgcagtgtaa
     6241 tcatcaggga tataactggg taacgctggg tcatccgcca cacagttcgc caatacaata
     6301 ttttcaccat acagagactc aggtagcgtg atatgtcgca gccccattgt agtatagata
     6361 atgccatccg gacggtgggc aagcagctga cgtgccgcgc gggcagcgtc atcttcagaa
     6421 aaaatattga ttaaaaaact attccagccg aactcgctgg cggtttgctc aatggcaagc
     6481 agaatatcaa cagagaaagg agtggtagca gtgtcctgcg ccagcacggc gagagtcgac
     6541 ggcttacgtc cttgagcgcg catcttacgg gcggaaagat caggaacata attcagggtc
     6601 tggattgcct gcaatacgcg gtcacgcgtt gcaggacgca cagattctgc attatgcatc
     6661 acccgggaga ctgtcatcat cgacactccc gccaggcgtg cgacatcctt taatgaagcc
     6721 ataccccata cccaagccgt ttgccgtaaa acgggcactg tagcagaaac agacgtcact
     6781 ggcgagatcc aacgaaattc tcgacgccct atcacccgac acggcaatac aataaaaaat
     6841 aacaataatt gccggataat tgtacccaat tccgcctctg ttctcgccaa cgagtgacca
     6901 tataggcaag taagaaaaac atgccaactg gcatcgccaa cctggagaag aaaatataaa
     6961 tatggatcat tgtgctgccg ctgtatcccg acatcagccc ggaaattatt gcgattgcta
     7021 tcggttcagg cgcgattggc tgcacgatcg ttacggactc gcttttctgg ctggtgaagc
     7081 aatattgcgg cgctacgctc aatgaaacat ttaaatacta tacgacagcg acatttatcg
     7141 cttcagtcgt cgctctggcg ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag
     7201 acgtactatg gaaaacgcta aaatgaactc gctcatcgcc cagtatccgt tggtaaagga
     7261 tctggttgct cttaaagaaa ccacctggtt taatcctggc acgacctcat tggctgaagg
     7321 tttaccttat gttggcctga ccgaacagga tgttcaggac gcccatgcgc gcttatcccg
     7381 ttttgcaccc tatctggcaa aagcatttcc tgaaactgct gccactgggg ggattattga
     7441 atcagaactg gttgccattc cggctatgca aaaacggctg gaaaaggaat atcaacaacc
     7501 gatcagcggg caactgttac tgaaaaaaga tagccatttg cccatttccg gctccataaa
     7561 agcacgcggc gggatttatg aagtcctggc acatgcagaa aaactggctc tggaagcggg
     7621 gttgctgacg cttgaagatg actacagcaa actgctttct ccggagttta aacagttctt
     7681 tagccagtac agcattgctg tgggctcaac cggaaatctg gggttatcaa tcggcattat
     7741 gagcgcccgc attggcttta aggtgacagt gcatatgtct gctgatgccc gggcatggaa
     7801 aaaagcgaaa ctgcgcagcc atggcgttac ggtcgtggaa tatgagcaag attatggtgt
     7861 tgccgtcgag gaaggacgta aagcagcgca gtctgacccg aactgtttct ttattgatga
     7921 cgagaattcc cgcacgttgt tccttgggta ttccgtcgca ggccagcgtc ttaaagcgca
     7981 atttgccgag caaggtcgta ttgtcgatgc tgataaccct ctgtttgtct atctgccgtg
     8041 tagtgttggc ggtggtcctg gtggcgtcgc attcggactt aagctggcgt ttggcgatca
     8101 tgttcactgc ttttttgccg aaccaacgca ctccccttgt atgttgttag gcgtccatac
     8161 aggattacac gatcagattt ctgttcagga tattggtatc gacaacctta ccgcagcgga
     8221 tggccttgca gttggtcgcg catcaggctt tgtcgggcgg gcaatggagc gtctgctgga
     8281 tggcttctat acccttagcg atcaaaccat gtatgacatg cttggctggc tggcgcagga
     8341 agaaggtatt cgtcttgaac cttcggcact ggcgggtatg gccggacctc agcgcgtgtg
     8401 tgcatcagta agttaccaac agatgcacgg tttcagcgca gaacaactgc gtaatgccac
     8461 tcatctggtg tgggcgacgg gaggtggaat ggtgccggaa gaagagatgg agcaatatct
     8521 ggcaaaaggc cgttaataac gtttcaacgc agcatcgcaa tcctttccct gggtgagcga
     8581 tgctgccgat ggcgcagact taagatcccc agtcttaccc gctataaccc cctgctttta
     8641 ccatcaccca acgcctttcg ctgtaaacgg cggtttcgca aaccaaacca aaaccgtaag
     8701 taagataaaa gcaatagccg ccatacggaa aatctcattt gcagaaatag aaagtgactg
     8761 ctgggttatt tcattattaa tttcattaag aactcctgaa agcgaaccat agtatttatc
     8821 cataatttgt gacgatgaat taaacacggg gttaaattga tcgatggttt ctgtcaactg
     8881 actatggtgt aacgattcgc gtcgtcccca cagcgtcatt gtcaacgacg taccaactga
     8941 tcctgacaag gtacgaaaaa aattactcat actcgaggca ttggcaaatt tattatctgg
     9001 caagcctgaa aacgaaatcg ttgttaaggg taaaaagaaa caggcaacgg cgaatccctg
     9061 aaaaaactgc ggcaaaatga tacctgtaaa atcaatcgtt ggcataaatg tcacagaacg
     9121 ccagtaatag caaaccgcat acatcaaaaa actgaatgtc accaacaccc gcatgtctat
     9181 tttgttgcca taacgtccta tcaaaggtga aattaatagt ggcatgatgc cgatgggcgc
     9241 ataagcaagt ccggcccata tcgcattata ccccatcgtt tcctggagta actgcggcat
     9301 aaggacgatc gctccagagt aaaataaata cgcgcatgtg atactcacaa taccaatggt
     9361 gaagttacgg gacttaaaca aactgagatc aagaatcggg ttctctgagg tcgactccca
     9421 aatgactaaa gagatcagaa aaataactga tactactgtt aatattatta ttgtactcga
     9481 gttgaaccaa tccagatcgc gccctttatc aagcataatt tgcaagccac caacaccgag
     9541 cactaacagg gtcagtcctg gtagattcat tttgaccggt gaagtctcag tttctcttcc
     9601 tttaagtaag gttaagcata atgtcaggac gataatcccc atagggacat tgattaaaaa
     9661 tatccaaccc cagctaaagt tatcacaaat ataaccgccc aatatcggcc cacatatcgg
     9721 agcgataatc acggtcattg accataatgc cagagcaaat gttctttttt ctggtggata
     9781 attccttaat aataaactct gtgacagtgg aattaacggc cctgccatta acccctgaac
     9841 gactctaaaa aatatcagca catcaagatt gatcgataaa ctacacatta aggaagacag
     9901 cgaaaaaaaa gtgactgaaa gtaaaaataa ccttaattcg cctattcttt gtgccagcct
     9961 gccggtaaca gggatcgcaa tggcatttgc tacgccaaac gaggtgataa cccaggtgcc
    10021 ttcgtctgtt gatgctccca gaaagccaga tattgtcggt attgcgacgt tagaaatagt
    10081 ggaatccaac atttgcataa acgtcgctaa tgacaatgca atagtgacgc accataacgt
    10141 cccaccggtt aatggtgccg gagttgattt agtgattgcc attaaagttg tccattatgc
    10201 gaaataatgt tgctaatttc tttttctatc ggactggtat cgataactaa agccttactg
    10261 gtataagccg gcatggacgt cacggttgaa gccagctcag gcatctcagc aatgtcttcg
    10321 ttcttcgtat caatagttgc agtcatcgat aaaccaatgc gcaaggggtg ttccatgagt
    10381 tcttttggat caagagaaac ttcaaccggt acacgctgaa cgattttgat ccagttccct
    10441 gtcgcatttt gtgcaggtaa taaggagaac gcattgccgg ttcccatatt gatccctgtc
    10501 acccggccat gaaacacaac attctcacca taaagatcgc tgataatatt gaccgattga
    10561 ccaatccgta catccgtgag ttgtgtttct ttaaagttag cattaaccca catttgacgt
    10621 gccggtacga cagccattaa cgattgtccg ggactcactg tttcgccgac ctgaacactt
    10681 ctctgggcaa tatagccggt aaccggactc ttaatatccg tacgtttaag cgccaaccag
    10741 gcttctttgg ttgcatccgc agcttcaacg acttgtggct gacggtttaa tggtgtgttc
    10801 attactaaag ctttattcgc tttataagcc tggatagcgg cattcaatgc cgctttgcta
    10861 cttattaacg tatctttggt atgctccagc gtttcttttg aaataacccc ctgcttcgct
    10921 aaaggcacac gacggttata atcttctaaa gattgttgat actgaatacg tgctgaagcg
    10981 acttcggcac tgtattgttt atcctgtaag tacagtttat tcgtttgccg aacaatattt
    11041 gccagattat ttttagcttt attgagtgcg atagtggcat cagttttatc cagtgaaact
    11101 aaaatgtcac cctgtcgaac gtagttcgta tctttatgat taacgacagt gacactacct
    11161 gagacttgtg cagaaattgg atctgcattc cccgtgacat aggcgtcatc tgtactaatc
    11221 atgtcttcta attccattga ccaataggca taagcacctg aaaacgcaat aaataaaact
    11281 atcgccaata aagaaaagta ttttcttctg ttagaatgtt ttttatttga attaatctgt
    11341 tccactatta tctctcattt cgcatagatg aaatttatga attgaatgaa aagagtatt
//
LOCUS       DAZFBR010000134        11315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFBR010000134 DAZFBR010000000
VERSION     DAZFBR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.134"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(262..1746)
                     /locus_tag="ACHJZ2_004143"
     CDS             complement(262..1746)
                     /locus_tag="ACHJZ2_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4570152.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKFRKEALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(1933..2886)
                     /gene="ompT"
                     /locus_tag="ACHJZ2_004144"
     CDS             complement(1933..2886)
                     /gene="ompT"
                     /locus_tag="ACHJZ2_004144"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO4570153.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            3385..3969
                     /locus_tag="ACHJZ2_004145"
     CDS             3385..3969
                     /locus_tag="ACHJZ2_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4570154.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(3994..4086)
                     /locus_tag="ACHJZ2_004146"
                     /pseudo
     CDS             complement(3994..4086)
                     /locus_tag="ACHJZ2_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            4083..4829
                     /locus_tag="ACHJZ2_004147"
                     /pseudo
     CDS             4083..4829
                     /locus_tag="ACHJZ2_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(5201..5467)
                     /gene="minE"
                     /locus_tag="ACHJZ2_004148"
     CDS             complement(5201..5467)
                     /gene="minE"
                     /locus_tag="ACHJZ2_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO4570155.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(5471..6283)
                     /gene="minD"
                     /locus_tag="ACHJZ2_004149"
     CDS             complement(5471..6283)
                     /gene="minD"
                     /locus_tag="ACHJZ2_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO4570156.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(6307..7002)
                     /gene="minC"
                     /locus_tag="ACHJZ2_004150"
     CDS             complement(6307..7002)
                     /gene="minC"
                     /locus_tag="ACHJZ2_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO4570157.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            7522..7890
                     /gene="ycgJ"
                     /locus_tag="ACHJZ2_004151"
     CDS             7522..7890
                     /gene="ycgJ"
                     /locus_tag="ACHJZ2_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO4570158.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(7993..8394)
                     /gene="pliG"
                     /locus_tag="ACHJZ2_004152"
     CDS             complement(7993..8394)
                     /gene="pliG"
                     /locus_tag="ACHJZ2_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO4570159.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            8465..8623
                     /locus_tag="ACHJZ2_004153"
     CDS             8465..8623
                     /locus_tag="ACHJZ2_004153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570160.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            8635..8928
                     /locus_tag="ACHJZ2_004154"
     CDS             8635..8928
                     /locus_tag="ACHJZ2_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO4570161.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            9000..9659
                     /gene="ycgM"
                     /locus_tag="ACHJZ2_004155"
     CDS             9000..9659
                     /gene="ycgM"
                     /locus_tag="ACHJZ2_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO4570162.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            9751..10197
                     /gene="ycgN"
                     /locus_tag="ACHJZ2_004156"
     CDS             9751..10197
                     /gene="ycgN"
                     /locus_tag="ACHJZ2_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO4570163.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(10404..>11315)
                     /gene="hlyE"
                     /locus_tag="ACHJZ2_004157"
     CDS             complement(10404..>11315)
                     /gene="hlyE"
                     /locus_tag="ACHJZ2_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO4570164.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGNIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AANKMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         2978 a         2390 c         2600 g         3347 t
ORIGIN      
        1 gctctgggta tgaagctgtt tgtaactcat gttacagtaa tggctattac tatctcgaag
       61 gtgcaaaatg tatattgatt tgatatttta taaatttact tcgaatctgg cacgtcaact
      121 tttgattaaa gtaatatcag ttttcggcga aaaaaggacc gtgtacatga gacgatttca
      181 tacaagaata acaaaaatat gaatatgctc ccaatgcaat atatgttttg cattgggagc
      241 aaatagcgga atattttgaa attattgtat ttctgcttca gaatttcctg ggcagtatat
      301 attttctatt ttttgtggag tgatcaggtg atgtacaact gctttatcta cacataaatt
      361 aactccggtg agagctaatg tatgcccatc accatttatt gttagtaacg ggctggaaaa
      421 tttctctgcc atcttacggg cattaatcca gggcgttgtt gggtcgtatt tgtgtgctac
      481 aaacagtaaa ccagagggca gaacagtatt tttcaggcga gttttgttca ggtcgctatg
      541 tattggccat aattcacaaa aatcaggtga atcggaacgt ccattgtcaa agttaatagc
      601 cgggaaggca ttcgcaagag cctcttttcg gaattttcgc tcttctggtg ttaattgctc
      661 atccccctga tctacacaaa ggattacccc cgaagcatca cttgactctt ctgaggctat
      721 cggagcactg agcgcagttt caatttcatt actgacaatc ccctgagaga actggcgtat
      781 ggcagttgca agggttggcc atgatgaacg ccatagcaga aggtctgttg ttaatgatat
      841 gagttcatct gaagatatat tttctccctt actgtctaat aaaggtttgt gatgtaattt
      901 tgataatagc tcatggaact gagttattgc cttatctctg tctgaagaaa gcgggcaact
      961 ttttgtacgc gcacaccagg atgcaaagcg atcaaacgtt tcctgataac tctgtgcctg
     1021 tttgagttgc catgtgaagt tgtcctccag gtcatcgata tcgacgactc catcaagaac
     1081 gatagatctt acgttgtagg gaaaacgttc tgcatataag gctgcaattt gagttccata
     1141 cgaatacgcc acggctgtca gttgtttatc ccccaaggct tgcctaatac gatcaatatc
     1201 gtatacagcc tcgttagagc cgatatggcg aatgacttcg gctccggtat tatggataca
     1261 ggcattaatt ttatttaata cttgttgctt ttcggttatg ttttcctgag tctctgtatc
     1321 tgattgccgg cagtttattg tcggagtgga ctgcccgacg cctcgaggat caaatccaat
     1381 aatatcccat gactcacgaa gatttgtgac tggccagtca aagttaatat aaggatttat
     1441 gcctggtaac ccgggaccac cacttattat caggatactt cctttatgct tgctttttgc
     1501 cggcaatttt gtcaacgcta gtttgacttg tgattttttt tcataagaag catctccgcc
     1561 tgtgtctgta tattttaatg gaacagacaa ataaccacat agtaagtcag gagacggttt
     1621 ttcctcacca aaccagtggt tgaattgact ggccatacag gattgccact gtatctgctg
     1681 ggcagatacg gttactggta gaagtaacgt taaaacaact ttgaaatgag taattatttt
     1741 tcgcattgtg tctctgaata tcggaataaa gataaaattt gaatatattg aggtcttgtg
     1801 ttgcggtaag agattacacg ttatgacata ggttaaatgc ttacaaaatt agtggatatt
     1861 gtctacttgt aactgtaaac aaattccccg gggttataca ataccaccgg ggagaaaatc
     1921 tggttaactt cgttaaaagg tgtacttaag accagcagta gtgatgaagt tatagttttc
     1981 tatgcctgca ccatttttgc tgtagtctga agtgttatca ttgtgatcat aaagtgaagt
     2041 attacctttt ttattcgtaa cccgattcca tgtgccttca acataaactt ttgcgttagg
     2101 tgttacgtaa taacctgcat tgactgaaac agaatagtaa ttttggtctt tgactttact
     2161 gcgataagtg attctttttc ctgggtcata gtgctcatcg ttatcagatg cttccaccca
     2221 gccgctgtat ttaaatgtgc cacctagctc aaaatcttca taacgataac ttccagtcaa
     2281 gccaatgtag ggcattttaa aacgttgttt gtagccgatt gctctttctc cattcggaaa
     2341 ggagccgata tcatctctga atccctcctc agaactgtag atataggaac cacctctggc
     2401 tgtaaagcta taacggcttt cctgatatcc ggccatgagt cccaggcggt aattgggttc
     2461 gttgaggagc cagcctttga tattcagatc aaattcgttg gcataattga gttgtgtatc
     2521 agggtgtcta ctttcatccg tccaggttcc ggggttactg gaatccatcc agtcctgatc
     2581 gaccatattg ccacctcggc tgccgagagt tgtccagcca gcagccccga tagatatctg
     2641 gggcatcaaa tcccaattaa ttgcaccttt aataattgca gcgttattga atttccagtc
     2701 gagttgactg acttttcggc ctccttcttc ggctagataa acacgctctt ttgtttttcc
     2761 gctcagagtt ccaagactaa tgtccgcatt tatgttgtca ggagtaaacg ataaagtctc
     2821 ggtagaagca aaagagctga tcgcaatagg ggttgtcagg actattccca gaagtttcgc
     2881 ccgcataaaa ggtctccatt caatcgtttt aatgattgaa tatgtatttt ttatatctaa
     2941 cttaatgagt caattgtata ttgtttaact gtttatggtt tgtttaagtt tgaattattg
     3001 tttcgtttta ttcattcttt atgggcagct tgccaagtgt tgtattattg ttgtggttta
     3061 atatgctgga aattttcttt ttgctgatat ttttatctgt ctatagttat tcatttatac
     3121 gcatattagc aaaaaaaata ttattttagg tatttctaca gtacatttga cacgatgatt
     3181 aacctctatg tagctaggtt tttgtaaaga aactgtgcgt cactgtatcc aggactgtta
     3241 tgtgccaaat aaggaagttg aagttaccag ctcttactct aaagctataa cgtatagaaa
     3301 aaatgcattt gagacgtgta aaatcattta tttaatcgca atttatgtaa tggcataatc
     3361 aggtcggtga aagaggggag aagaatggat aaaagagcta aaaatcagat agtcgatagc
     3421 gatattgcac gtttgttact caagctcaga aagtctcgaa acttgactgt tacagagctt
     3481 gcccaacgat ctggtgtcag tcaggcgatg atcagcaaag ttgaacgagg cacttcaagc
     3541 cccagtgcaa caatacttag tcggttagct aatgcactga atattacgct atcaaagctt
     3601 tttgcagagc ttgagatgca acagaattca cttgtacttt tggctgacca gcaacaacat
     3661 tggattgatg aagaaacagg cattacccgc tggtctttgt ccccagcggg tgcatgtccg
     3721 gaactaataa aagttgaaat cccccctatg gggcaattga ctatccctgc ctctgcaaat
     3781 gagcatcttt ctggacaaac tctatggatg ttaagcggca gccttgattt tcgagtcaat
     3841 catcagactc atcatttaca agccggtgat tgtcttgctc ttacctttgc cagcgagtac
     3901 caaatgagaa acccagatag cggccagagt tgttcttaca ttgtcgcttt cagccaaaaa
     3961 aatccatgat gtaattctcc taaaattatc atgtcacatt gcgttataaa gatgtaatag
     4021 agggtagggt ctgccttgat tgtcccgctc ggaacgtcct gaaagtttaa agcccatatg
     4081 ttgatgcggg tgccgggttt gagcagacgc tgaccggaat gacagtgggg atcgacagcc
     4141 gtaatgatat tcctgagggg attgcgacgc tgggcgcttt tatgggttat tcccattcac
     4201 atatcggttt tgatcgtgga ggacatggca gtgtggacag ttattctctg ggcggctatg
     4261 ccagttggga acatgaaagt ggtttctatc tggacggtgt cgtgaagctg aaccgttttg
     4321 aaagtaacgt agccggtaaa atgagcagcg gtggagccgc caatggcagt taccatagca
     4381 acgggctggg cggtcacatt gaaaccggga tgcgatttac cgatggtaac tggaacctga
     4441 cgccgtatgc ctcgttaacg gggttcaccg ctgataaccc cgaatatcat ttatccaatg
     4501 gcatggaatc gaaatcagtc gatacccgca gtatatatcg tgaactgggt gcaacgctga
     4561 gttacaacat gcgtctgggg aacggtatgg aagttgagcc gtggctgaag gcggctgtgc
     4621 gcaaagaatt tgtcgatgat aaccgggtga aagtgaatag tgacggtaat ttcgtcaatg
     4681 atttgtcggg cagacgtgga atataccagg caggtattaa agcctcattc agcagtacgt
     4741 taagcgggca tcttggggtg gggtatagca acggtgctgg tatggaatcc ccgtggaacg
     4801 cggtggctgg tgtgaactgg tcgttctgac catcaacgat taaactgcgc ttcggcgcag
     4861 ttttcgttta caggatgtta aaagagaaaa ttctggggta aaaaaagccc gccagttacg
     4921 gcgggaaacc tcatcctatg ggagaacaat gaataatgaa attgcggggt tatcatctcc
     4981 cagtatatca acactaacaa taaggttatt tactcaacca ggcataaata ttttgttttg
     5041 tgcgtgggaa cagccttaag gtgtaaaggg ggaggtggaa atagcaatga ggagtatcag
     5101 caagaatact cgccgcttta ccacaacgtg gatgagaggg atgaaaaact caaggcagag
     5161 ataactctgc cttgaagata aatgcgcttt tacagcggga ttatttcagc tcttctgctt
     5221 ccggtaaggt cacgttcagc tcaagaatag aaatatcgcc atctttttgc tcaagctgta
     5281 cggttaccat ctcaggatca atttgtacgt atttacaaat gacctcaaga atatctttac
     5341 gcaactgcgg cagataatgc ggttctgcat cgctgcgacg gcgttcagca acaataatct
     5401 gcagccgttc ttttgcaatg ttggctgtat ttttcttccg cgagagaaag aaatcgagta
     5461 atgccataac ttatcctccg aacaagcgtt tgaggaagcc tttcttctct tcttcaatga
     5521 agcggaaagg acgttcttct cccaacagac gttctacggt atctgcgtag gctttacccg
     5581 catcggcgtt aatgtcgaga atgaccggtt caccctggtt agaggcgcgc aatactgatt
     5641 gatcctctgg gatcacgccg acgagtttga tgcgcaggat ctccagcaca tcttccatgc
     5701 tcagcatgtc acctctgctt acgcggcctg ggttatagcg cgttaacagc aggtgctctt
     5761 taataggctc ttcgccattt tctgcgcggc gtgattttga cgccagaatg cctaaaatac
     5821 ggtcagagtc gcgtactgag gagacttccg ggttggtggt aataatggct tcgtctgcaa
     5881 aatagagtgc cattaacgca ccggtttcaa tccctgccgg ggagtcacaa acgataaatt
     5941 caaaatccat cgctttcaga tcatcaagaa ctttagcgac cccttcacgg gtgagggcat
     6001 ctttatcgcg tgtttgcgat gccggcagaa tatagagatt ttcagtacgc ttatctttaa
     6061 ttaacgcctg atttagcgtt gcatcgccct gaatgacgtt gacgaaatcg taaacgaccc
     6121 ggcgttcaca acccataatc aggtcgagat tacgcaggcc gatatcaaaa tctatcacga
     6181 cagttttctt tcccttctgg gccaaaccag tggcgatggc cgcgctggag gttgtcttac
     6241 caacaccccc tttgcccgaa gtaacaacaa taatgcgtgc catagaaatt ccttgttaaa
     6301 aagggatcaa tttaacggtt gaacggtcaa agcgttttcg actaactgca gtcgcgccgc
     6361 tttgccataa aattctgctg ggatttgatc actcagccag tattcacctg cgatggacac
     6421 caattccgcc atcaggttcg tacaaaatat ttgcgtttcc cggtcaccac ttgcccctgc
     6481 cagcgcacga ccgcgcatca tgccatagac atgaatgttc ccatcggcaa tcaattcggc
     6541 cccagcgcta acgtggcttg taacaatcag atcacattgt ggagcataaa tacgctgacc
     6601 ggaacgcacc ggggtatcta ttaaacgcgt ttttgtgacc ggcgttgtat tttgcgctgg
     6661 agcctgcggt gcgggagctg gacgtggcgc tttttccttt ccttccgtca ggataggcag
     6721 ccccattttt tcaatttcgg ctttaagttg cgcatctttg cagccactta cgccaataac
     6781 ccgcaaaccg gttgccgaaa ccgccttatg catcgctgac cagtttaccg ggtcttccag
     6841 tgcactgacg ttgagtacaa cgggggcatg ttttaaaaat gcgggggcct gagcgatttt
     6901 gtcttccagc gcctgatgga taaccttagg ttctgcctca tgcagatgaa ccacagataa
     6961 agtgaagcta ctgcctttaa gctcgattgg cgtgtttgac atcctggcct tactcaatta
     7021 gctattaatc atcgccagcg cgcgatgatg ttccgaagac tataaggcat gttatagtct
     7081 ggattatatt gaggcaagtc accctcccat ttattcagag taaaagtcta ttctgtgata
     7141 aatggcgctg attcatagct taaaaaatac ccttgtcaat caacccattg ccgtcgtact
     7201 tttgattctt cttatttacg cttccttttt ccgcacccta atcgtgacag catctccaga
     7261 cctgtttaaa tatccagtat tgaggcaagt gtattttttc accacgccag gctttcttaa
     7321 ctttccattt tttgcagcat cattaaagaa cagatacccg ttatcttaat atccgccaca
     7381 gaattgctct atcatagaaa ccgcagaaga cgtctgatac ttctgtcagg tcatcgtaac
     7441 tcgtccaggt cagcggtttt gattacggat tgatgatgaa atgagcataa gtgttgtacg
     7501 gttggattat caggatgtgc tatgaatatg atgcgtattt tttatatcgg attgtcaggt
     7561 gtggggatga tgttctcatc aatggcgtct ggacatgacg ctggcggact tcaatctccg
     7621 gcgtgcggcg ttgtttgcga cccgtatatt tgtgtgaact cagatggcat ttccccagag
     7681 ttaacaagga aatatctcag cgaaaaagcc gctgaaaact tacaatcatt acaaggctac
     7741 gatcccagcg aatttacatt cgctaacggt gtattttgcg atgttaaaga aaaattatgc
     7801 cgtgatgatc gttattttgg tgtagatggt aagcggagtg gaaaaatcaa tcaaaccacc
     7861 acaaagatgt tatttatgtg tcgtgagtga tgtcttgttt acattatgtt ctattcgtca
     7921 ttaacctctt gagcgacaga gaggagtggg gcgtcaacgt ttatgacggg gagactcccc
     7981 gtcggttgac atttacttaa tttgaatatc gacgttatat tttttagttt tgtttttacg
     8041 ggcgtcgttt cgggtttgca gcactcgcag ctcatatttg ccagaagcag ggagtgagta
     8101 ttgtccgtga ctatcgagtt caggggaata tctggataaa tcaacagaat catcgattcc
     8161 tggcccgaag aggtaagtat cagcgccttc attagagata ctcacatgca ctttctgacc
     8221 ttttttggca tagaaggtat atgtatcgta atcgtatccc tttatttcgc cggagtactg
     8281 agcgctgcta tggccttttc tgaactcaac attgacattc ttacccgcag caaagctcgt
     8341 ggaggttagc agtgcaagca gcaatacagc cttactgatg ctcttgattt tcattggtag
     8401 tcttctgttc agaatgagta cgatgaactg ttataatata acaatccctt acgggagaat
     8461 tccagtggct ttcctgcgtt cggtaaaatc ccgtcgcggc atttttgccg gggggattac
     8521 ttataaaggg gcatggactc aacaaaccgt agaaatttac agctggctgg cttgtggcgc
     8581 gggtttcatt gatgttagct tatgccaaaa cctggcatct taaagagtaa aagtatgttt
     8641 tgtgtgattt atcgaagcag caagcgtgac cagacctatt tatatgtcga aaaaaaagac
     8701 gatttttcgc gtgttcctga ggaactgatg aaaggttttg gtcagcctca gttagcgatg
     8761 attctgccgc tggatgggcg gaagaaactg gtcaatgccg atattgaaaa ggttaaacag
     8821 gcattaaccg agcaaggtta ctatttgcag ttaccgccac cacccgaaga tttgctgaag
     8881 caacatcttt ccgtcatggg gcagaaaaca gacgacacta acaaataacc gatatccggc
     8941 ggtggcatta tctttgtcgg cgcgggtttt catatccacg ataaggtgag gggaacgtta
     9001 tgtatcaaca tcacaactgg caaggtgcgc tgctggatta tccggtgagt aaagtagttt
     9061 gtgttggcag taactatgcc aaacatatta aagagatggg cagcgcagtg cccgaagagc
     9121 cagtgctgtt tattaaacca gaaacggcac tgtgcgatct gcggcagcca ttggcgatcc
     9181 catccgattt cggttcagtt catcatgaag tcgaactagc ggtgttgatt ggcgcgacgc
     9241 tgcgtcaggc tacggaagag catgtccgca aagccattgc cggttacggc gtggcgctcg
     9301 atctgacgtt gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccg tgggaaaagg
     9361 ctaaagcgtt tgataactct tgtccgcttt ccgggtttat tcccgcggcg gaattcaccg
     9421 gcgatccgca aaatacaacg ctgggcctga gcgtaaacgg cgaacaacgc cagcaaggta
     9481 cgactgcgga catgatccat aaaatcgttc cgctgatcgc ttatatgagc aagtttttta
     9541 ccctcaaggc cggtgacgtt gtgctgacag gcacgcctga tggcgtcggc ccgttgcaaa
     9601 gcggtgatga gctgacagtc actttcgatg ggcattcttt gacaactcgc gttttgtaat
     9661 actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc tgtgccagac tggttataag
     9721 gtgcgtttta acgtaatggc ggaacacctg atgagcgatg tacctttctg gcaaagtaaa
     9781 accctggacg aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg cggtcagtgt
     9841 tgcctgcata aactgatgga tgaagacacc gacgaaatct acttcactaa cgtcgcctgt
     9901 cgccagctca atattaaaac ctgtcaatgt cggaactacg aacgtcgttt cgagtttgaa
     9961 cccgactgca ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct accaatgacc
    10021 tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg cgtggcatcc gctgcttact
    10081 ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc gtcatatcgc agtgaaagaa
    10141 tcagaagtga ttgactggca ggatcatatc ttaaataaac ctgactgggc gcagtgataa
    10201 atttaataga tgttgatggg ggctttctta atatacgtct ggatgctcgc catttcgtag
    10261 ccctgttttt gaaagcgctc tgtcataatg agagttaccc gataccttat tatcaacagg
    10321 taactctcaa tctttaaaaa acgtctgtat ttttaaatgt gataaacttt ataccttgag
    10381 tacatggaga gaataattgc tgatcagact tcaggtacct caaagagtgt cttcttaccg
    10441 tgtcttttct gatactcatt acaggtgtta atcattttat tggccgcttc ttttagcaaa
    10501 gaaagcatta aatcatcata atcaacatag aatctggttg tttcagtttc cgtttttatc
    10561 tccccgatgg cggctatttc ggtggttaat ttcaatttgg cggcatcgat atctttattc
    10621 gcttgtttaa ccgtgttaga cagggtggta aagaaactct gcacagattt taacttgttc
    10681 ttcaattctg gaatcagttt cccttcaact acgcccgcag caatagaata ggaaatgatt
    10741 aaaccaaatg gaccggcgac gacaccggct gcggcaccgg catacgcttc cttcctgatt
    10801 ttatctacct gtgactggaa atagctgctt ttttctgaaa aatcattggt taactggcta
    10861 tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt gtgagcttac cagcagggat
    10921 ttttgcgctt cattcagctt cgtgatgccg tcatccagta ccttaatgag aatgtctttc
    10981 tgggcggatg ctttcttctc attatactca tcaaatagca aaatatacgc tgcgagcaat
    11041 tgcgtcgcaa caccacacca ttcatacact gtttgggttg cttcaaaata cttatcctgg
    11101 ctatccatta gtaaggtttt aatattgcca actaaaacgg aggctgcctg tgaatactcc
    11161 tgtttaaagc gacttaactc ttttatggtt tcatcaaagg tctgccaggg gatgacctga
    11221 tcgagatatt tattataaag atccaatgct ccatctgcgg tttcgattgc gtttttaact
    11281 acctctaccg ttttatctgc aacgatttca gtcat
//
LOCUS       DAZFBR010000135        11205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFBR010000135 DAZFBR010000000
VERSION     DAZFBR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.135"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            415..1065
                     /gene="csgD"
                     /locus_tag="ACHJZ2_004158"
     CDS             415..1065
                     /gene="csgD"
                     /locus_tag="ACHJZ2_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO4570165.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1070..1459
                     /gene="csgE"
                     /locus_tag="ACHJZ2_004159"
     CDS             1070..1459
                     /gene="csgE"
                     /locus_tag="ACHJZ2_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO4570166.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1484..1900
                     /gene="csgF"
                     /locus_tag="ACHJZ2_004160"
     CDS             1484..1900
                     /gene="csgF"
                     /locus_tag="ACHJZ2_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO4570167.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1927..2760
                     /gene="csgG"
                     /locus_tag="ACHJZ2_004161"
     CDS             1927..2760
                     /gene="csgG"
                     /locus_tag="ACHJZ2_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO4570168.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     QTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2824..3315)
                     /gene="ycdZ"
                     /locus_tag="ACHJZ2_004162"
     CDS             complement(2824..3315)
                     /gene="ycdZ"
                     /locus_tag="ACHJZ2_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO4570169.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(3417..3971)
                     /gene="ycdY"
                     /locus_tag="ACHJZ2_004163"
     CDS             complement(3417..3971)
                     /gene="ycdY"
                     /locus_tag="ACHJZ2_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO4570170.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3995..4732)
                     /gene="ycdX"
                     /locus_tag="ACHJZ2_004164"
     CDS             complement(3995..4732)
                     /gene="ycdX"
                     /locus_tag="ACHJZ2_004164"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO4570171.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4787..5725)
                     /gene="ghrA"
                     /locus_tag="ACHJZ2_004165"
     CDS             complement(4787..5725)
                     /gene="ghrA"
                     /locus_tag="ACHJZ2_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO4570172.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5959..6046
                     /locus_tag="ACHJZ2_004166"
     tRNA            5959..6046
                     /locus_tag="ACHJZ2_004166"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5993..5995,aa:Ser,seq:gga)
     gene            complement(6196..7038)
                     /locus_tag="ACHJZ2_004167"
     CDS             complement(6196..7038)
                     /locus_tag="ACHJZ2_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4570173.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(7123..7320)
                     /locus_tag="ACHJZ2_004168"
     CDS             complement(7123..7320)
                     /locus_tag="ACHJZ2_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4570174.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7332..7823)
                     /locus_tag="ACHJZ2_004169"
     CDS             complement(7332..7823)
                     /locus_tag="ACHJZ2_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4570175.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7820..8194)
                     /locus_tag="ACHJZ2_004170"
     CDS             complement(7820..8194)
                     /locus_tag="ACHJZ2_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4570176.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(8241..8615)
                     /locus_tag="ACHJZ2_004171"
     CDS             complement(8241..8615)
                     /locus_tag="ACHJZ2_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO4570177.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGFCGYVYLAVYPTPAPATTS"
     gene            complement(8778..8999)
                     /locus_tag="ACHJZ2_004172"
     CDS             complement(8778..8999)
                     /locus_tag="ACHJZ2_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4570178.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(9062..9538)
                     /locus_tag="ACHJZ2_004173"
     CDS             complement(9062..9538)
                     /locus_tag="ACHJZ2_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO4570179.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRGPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9554..10039)
                     /locus_tag="ACHJZ2_004174"
     CDS             complement(9554..10039)
                     /locus_tag="ACHJZ2_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4570180.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNLPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(10130..10948)
                     /locus_tag="ACHJZ2_004175"
     CDS             complement(10130..10948)
                     /locus_tag="ACHJZ2_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4570181.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT         2862 a         2769 c         2760 g         2814 t
ORIGIN      
        1 taaaaaattg tccacggtgg tgtggagaaa aaacaagaac gttttacatg acgaaaggac
       61 tacaccgaaa tattttttat atgcattatt agtaagttag caccatttgt atgatttttt
      121 aaaattgtgc aataaaaacc aaatgtacaa cttttctatc atttctaaac ttaataaaac
      181 cttaaggtta acattttaat ataaccagtt acacttagtt acatgtttaa cacttgattt
      241 aagatttgta atggctagat tgaaatcaga tgtaatccat tagttttata ttttacccat
      301 ttagggctga tttattacta cacacagcag tgcaacatct gtcagtactt ctggtgcttc
      361 tattttagag gcagctgtca ggtgtgcgat caataaaaaa agcggggttt catcatgttt
      421 aatgaagtcc atagtattca tggtcataca ttattgttga tcactaaacc ttctttgcag
      481 gcgacagctc tcttgcagca ccttaaacaa tcgctggcaa ttacaggaaa attacataat
      541 attcaacgtt ctctggacga tatctcttca ggctctatta ttcttctgga tatgatggaa
      601 gcggataaaa agcttatcca ttattggcag gatactttga gcaggaaaaa caacaatatc
      661 aaaatattgt tgctaaatac gcctgaagat tacccgtacc gcgacattga aaactggcct
      721 catatcaacg gcgtttttta tgccatggag gatcaagaac gcgttgtcaa tggattgcaa
      781 ggcgtcctgc gcggcgaatg ctactttacg caaaagcttg ccagttacct gattacacat
      841 tctggtaact atcgttataa cagcacggaa tcagccctcc ttactcatcg ggaaaaagag
      901 atcctgaata agctgcgtat cggcgcgtct aataacgaga tcgctcgttc gttgttcatc
      961 agcgaaaata cggttaaaac gcatctttat aatcttttca agaagatagc cgtaaaaaac
     1021 cggacacaag cggtttcctg ggcaaacgat aacctcaggc gataaagcca tgaaacgtta
     1081 tttacgctgg attgtggcgg cagaatttct gttcgccgca gggaatcttc acgccgttga
     1141 ggtagaagtc ccgggattgt taactgacca tactgtttca tctattggcc atgattttta
     1201 ccgagccttt agtgataaat gggaaagtga ctatacgggt aacttaacga ttaatgaaag
     1261 gcccagtgca cgatggggaa gctggatcac aataacggtc aatcaggacg ttattttcca
     1321 gactttttta ttcccgttga aaagagactt cgaaaaaact gtcgtctttg cactgattca
     1381 aactgaagaa gcactaaatc gtcgccagat aaatcaggcg ttattaagta cgggcgattt
     1441 ggcgcatgat gaattctaaa taaaaaattg ttcggaggct gcaatgcgtg tcaaacatgc
     1501 agtagttcta ctcatgctta tttcgccatt aagttgggct ggaaccatga ctttccagtt
     1561 ccgtaatcca aactttggtg gtaacccaaa taatggcgct tttttattaa atagcgctca
     1621 ggcccaaaac tcttataaag atccgagcta taacgatgac tttggtattg aaacaccctc
     1681 agcgttagat aactttactc aggccatcca gtcacaaatt ttaggtgggc tactgtcgaa
     1741 tattaatacc ggtaaaccgg gccgcatggt gaccaacgat tatattgttg atattgctaa
     1801 ccgcgatggt caattgcagt tgaacgtgac agatcgtaaa accggacaaa cctcgaccat
     1861 ccaggtttcg ggtttacaaa ataactcaac cgatttttaa gccccagctt cataaggaaa
     1921 ataatcatgc agcgcttatt tcttttggtt gccgtcatgt tactgagcgg atgcttaacc
     1981 gccccgccta aagaagccgc cagaccgaca ttaatgcctc gtgctcagag ctacaaagat
     2041 ttgacccatc tgccagcgca gacgggtaag atctttgttt cggtatacaa cattcaggac
     2101 gaaaccgggc aatttaaacc ctacccggca agtaacttct ccactgctgt tccgcaaagc
     2161 gccacggcga tgctggtcac ggcattgaaa gattctcgct ggtttatacc gctggagcgc
     2221 cagggcttac aaaacctgct taatgagcgc aagattattc gtgcggcaca agaaaacggc
     2281 acggttgcca ttaataaccg aatcccgctg caatctttaa ctgcggcaaa tatcatggtt
     2341 gaaggttcga ttatcggtta tgaaagcaat gtcaaatctg gcggggttgg ggcaagatat
     2401 tttgggatcg gtgccgacac gcaataccag ctcgatcaga ttgccgtgaa cctgcgcgtc
     2461 gtcaatgtga gtaccggtga gatcctctcc tcggtgaaca ccagtaagac gatactttcc
     2521 tatgaagttc aggccggggt tttccgcttt attgactacc agcgcttgct ggaaggggaa
     2581 gtgggttaca cctcgaacga acccgttatg ctgtgcctga tgtcggctat cgaaacaggg
     2641 gtcattttcc tgattaatga tggtattgac cgtggtctgt gggatttgca gaacaaagca
     2701 gaacggcaga atgacattct ggtgaaatac cgccatatgt cggttccacc ggaatcctga
     2761 cagataaaaa tgccggaatt aataatccgg catttactgt ttaagcgaaa gcgtgagtgc
     2821 ccctcacgct ttatttttga tttcctgctc acggagagcc gtctttgcac tacgcgccgc
     2881 cagccacagg ccgctgtttt tcattgcgta accaaatatc agcccaagcg ccagcgaagg
     2941 taacaccagt ttccagtcac cctgcccggc aaacgtcgcg catgcgccta taaacgtccc
     3001 cggaacaaat gaaagcagca gctgtttggc ctgaatacac atcagaaacg cgacaatacc
     3061 ggttatgaca taactgagaa tttccagatg tggcgccagt gcactaccgt aaataatgac
     3121 catcgcccac accacgccac taagcagagt tgcagcggag atcgccagcc ctttcagccc
     3181 accttgcggg caggcaaagt aggccgtaca gcccaggaag cccgcccagc ttagtaagcc
     3241 cagggaaaca gccacccatc cccagatacc ggagagaatg cctgttgtga ttgcaataga
     3301 gagaagtatg ttcatgccgc gcatgatagc agaaaacgcg gccatgaatg agatcgagca
     3361 cacattttat gcaacagcaa attatctgtt gcagttatga gtgacgcata tcacatttac
     3421 tcttcagaat cttcttccag ctcgtcccac attgcactaa tggcatcgcg ggttagcggt
     3481 gccatggtgc gccagaaagg cgtggttgca tgggcctcca ctttgccaag gaacgcacca
     3541 caccagggta acagatactc actgaacagt gtttccagtg cttcgctctc atcttccgtt
     3601 gactgatctt ccagccagga agccgcaagc agcaatgtgc cgatgtgatc ggcaggcgta
     3661 tccgctaatg gcatccctcg ttcagaaaga aaagcgcgca cttccgcttc cgttgcgtcc
     3721 tcaacccatg cgctacgata tggcggcacg gcacattcat cgccgataaa caacgcattg
     3781 taatcggcag agacttgcgt catatcacag cttttctgca aacgtgtcag caactcatcc
     3841 tgctccagtg gccagttcgc agccagtttc ccctcacgaa tcagggtaaa cagcggcacc
     3901 agtaaaggat cttgcggttg gcggtaatag agcgaaccca gcacacgaca gaggatagaa
     3961 aactcgttca ttaatatatt tccattacta gtaattaaag gtctgcaaat tccgcaatcg
     4021 gtgccatacc gcgagattca aggaagttca gtaagcggcg cggagaaaca ttcaaaatgc
     4081 gctctggcgg aaaatctacc gcgtcgagga ttttaagaca ctcttcaaat tcccccatgg
     4141 taaacgcggt gtgagaatcc gagcctaatg ccacccaacc tccagcatca cgtaccgctg
     4201 cggctaccgc acgacagttg tcttcactgc ccttacgtga gtgtaaaaat gaggaattat
     4261 tgatttccag cgccacctga tgtttcgcgg ctgcttcagc aacggcttta acatctatct
     4321 catatttcgg atttccggga tggcttatta tatgcacatt gccgctggcg atagtggcga
     4381 tcatcgcttg tgtattggtc gctttgtcat gtggtgcaaa aaccggctca tgaaaaccgg
     4441 caataatcag atccagcgag tcaaacattt taccgctgca atcaatttca ccatcaacat
     4501 ttttaatgtt agcttcgatg ccgcgcagga tccctacccc atcaaccact cgcggccaga
     4561 tacgcatgtt aatgaagtgc cagtgatgcg gcgcatcttc catgtccggg ccatgatcgg
     4621 taatcgcaaa aagtttaatg cccttttgtt tggcctgggc aatgtaatca ctcaatgtgc
     4681 tatatgcatg tgtgctggca acggtatgca tatgaaggtc gacgggatac ataactctct
     4741 cctgtattca attcccgcca aggatagcag gaatcctgat gctttattag tagccgcgtg
     4801 cgcggtcgac ttgcccgcag accctctccc ctttttcgag ctgggcaata gtgcgagaaa
     4861 tgtactccac agcttcagcg ggacgggtaa tcgcggcgac atgtggtgtt atcgtcacgc
     4921 gtggatgttg ccagagcgga ctttcaggcg gtaagggttc acgattaaaa acatccaaca
     4981 ttgcgccttt aactttgccg ctatccagcg ccgcgagcag gtcatcttcc acgacatgaa
     5041 caccacgcgc caggttgagg agatacgcgc catccggtaa tttttcgagt aattgttgat
     5101 taataatgcc gacggtttca ggggtattcg gtaacaaatt aatcaatacc cgacattggc
     5161 tcagaaatgc agacagttct tcccatccgg caaagctttg cacgccaggc cacgatttac
     5221 gggttcgact ccagcaacgc agcggaaagc gccaggtttg cagactctga gcaactttac
     5281 tgcccagtac gcctgcgccc aaaatgccga tggtaaaatc ttcccgatga tattcaggca
     5341 gcggttgcca atgcgaacta ttttgctgga tgcgataatc gtcaaaacgt cgaaaccaat
     5401 gcagcacctg actgacagca tattcctgca tttgctcgcc cataccggta tcttccaggc
     5461 gaaaaagtgg aacagaaggc ttcagcattt cagggtgtgc ctgtagcttg ctcaaaatag
     5521 aatcaacacc ggccccgagt gcgaacaccg ctttaagatc gcgccctgcc agcatttcaa
     5581 caggaggatg ccaggctaaa gcataatcag cagagtcatt atctccgctt ttccatgctc
     5641 tgactcttgc ctgaggaata gctttgcgca gtgcctctat ccaccattgg gtatcgaacg
     5701 ttgggtgata aaagatgata tccatactga ctcccgaaaa gcgttgtgcg aaatttattc
     5761 gcacttatcg ttatgatcta caaagaccac cagcataaca aatccgtggt cggtggccaa
     5821 aaaagcagat tttgcttatt aaaactgcat attgattgaa gtttgaataa acgcgcgatt
     5881 ttttaaaaaa gtttgttgac ctcaggtcat gatttcccta aattagcgcc cgttccagca
     5941 agacaggaac gacaatttgg tgaggtgtcc gagtggctga aggagcacgc ctggaaagtg
     6001 tgtatacggc aacgtatcgg gggttcgaat ccccccctca ccgccagatt ataagcgcat
     6061 gttattgttt aacatgcgct tttttatttt cccttccaga cagtatgcca tcgggtatgc
     6121 catctgaaac cggtttatat gccccgcgat catatcttat cggacaattt ccgatctttg
     6181 agcatactgg caaagctata ctgccagcat atcaggatag tgttttgcga taatatcatt
     6241 cagtctgtca accagttctg gttttctgaa cgtgatatgc gcggagccct tcttaaagta
     6301 tctgatgctg aacatctcat cttcatagct ctctttaccc tgaacagact gaatgtgatc
     6361 atccagacga agggtgatgt ttccccggtt gtcggggatt ggtttaccgc tgaacaggtg
     6421 caacattctt tccagatcgg cgagtcggtc agcctgttgt ccggtgataa gatgaaatcc
     6481 ccaccggtcc catctcacca gattgttgac gataatttta ctgccaaatt tacaggggca
     6541 attggttttg taattccagt tcagccctct gaagacgttg atcacccctc gctcaaacac
     6601 ctcatcctta ttctggtgca gttgttcaaa tgtgctcaga atgttcgctt cgctgatttc
     6661 cggaaaatta tcgtactcca gtgaccggta ccatgtatca cgggcctgcg tatccattaa
     6721 tgacagcatc ccggaacgtt gcatcaggtc acgccagatt tcgcgatcaa gattccgggt
     6781 aatggctttc attgctgttt cgggtttctc catcagccag cagccgcagc gaaaatcctg
     6841 tttcatggcc cagtccgggg cagttttacc tccgatgctt tccgtcagca ccgagatttc
     6901 agccagttgt cggataagtg tgtcgatttg caacagtgta ctctttcgta cagccagaat
     6961 gtggcgaatg ctggttgagc agataacgtc cgtatgcccg ttgaccactt ccggacggga
     7021 gattgtgata tctgacataa tcgatattcc tttttgcaga taaatgaaaa cgctagccct
     7081 gtgcaggctg gcgtcagagg aatgaagtct ggtgagtgag cttcaccggt tctggtgcag
     7141 aagctgcaga tgacgcaggg tacggacatc ctcccgggcc tgttcgatac agggcaacag
     7201 tgctgctgaa tccgttttat cctcatcgtt gtcaaagata attcccgatc cgcagtcgat
     7261 attgtcctgc agccacgcaa tcagaatatc cagcgctgtt tccgtggtta atgatttcat
     7321 gttgttcctg gttaccagtc gaaagtgggt aaacccggca ggcattcagc gctggcatcc
     7381 agatgaatga gactgacgcc ataacgccgg atgagtgtga cgaccagacg acggaacgtt
     7441 tttgacagac ccaggcgttt aagccgcaga accgggtacg accacgcatc ggtacgcaac
     7501 agatagccgt taccggtaaa atgaatccat tctgattcac cgaagtcact ggtctggtgt
     7561 gacagcgagt acagccaggc gttgtccgtt tccgtgatat gtgcggtact gcagcgtatg
     7621 ccggtaaggg tagcaaatgg tggcgtgcat tctgctgagg tctggtcaga ttcatcaaca
     7681 acacgaaggg agtagccgtt atcgcagacc atgttaatca gttcagcgag ctcaatacca
     7741 tcgacgtcaa cgacaatgcg ccccatgttc agtgcctgta cgttaacgct gtcggcttcc
     7801 agtgtcaggg ccagtttcat catttcgcct ccggatactt acccagggta atgttattta
     7861 ccgttctgta attgtcgcgg gtcatcaggc cggtcgcccg gcgagcccgg aggatatcaa
     7921 tactgtttat taactgagag cggggacagg tgctgaatcc cggctggtcg gtacgcacca
     7981 gcgcgtattt ttccacgaga aaattaactg catcacacag tgaaatgcct gcctcaatat
     8041 gctgctcaat cacacgttca tcggcaaacg gtgtgtcatt cagtgtgagg ccataatgct
     8101 ggtccagcag tcggatgagc agtgtctgcc agatggtgac gggagacggg cagcgagata
     8161 cctcccgtac atgtgtgtcg gataatgttt tcatcttaag ttctctgtaa ctggtagtgc
     8221 taaaggtgag agcttcgggg ttatgaggtg gttgcgggtg ctggtgtcgg ataaacagcc
     8281 agataaacgt aaccacagaa gccgagggtg tcggcttcgc aggtcagccc ttttgcattc
     8341 agcgtgacgg tatgctgata tcggggattc agttcaccgc tggtgagcat gagttccagt
     8401 tgtttcatca gcagcggaaa ggcctggtcc agatggtatg catctgcgtc gctgaagcgg
     8461 cctctgatac cggcgcggtc ggcaaggtaa tgcaggcggt taccctcttg caccagacga
     8521 gcgccaaaac agggcctcac tgtgcaggga agcccccacc aggggcggtc gtgattgtca
     8581 tcgggatgag ttatcccgga gagtttgtct gacacgataa aatcctctct gaaaagtggt
     8641 taagaatggt ttaacgatga caggtcagcc ggcgcgctga ccggtttcgg tgaagatgta
     8701 tgcattgatg agaatggcct catccacagc ggcgtctgag gtcatctggt cgtactcgtt
     8761 tcccgggtga cggaacatca gttcagggtg acactcatca gccggacata cgggccaaaa
     8821 ctgtccttac ggcgttctgc aaacacggca agaacgccgg gaatatcctg cacttcacga
     8881 ccagtatacg cttcagcact gccgtgccag cgatacttac cggtacagaa cggaaaaaga
     8941 cgggatgcag gatgctgttg gtgaatacgc atggcttcac cacgggtgat gattctcatg
     9001 atgtgatacc tctgaagaca gaagataaaa gtgaaaacag gtgtgatgtg gttgtgacgg
     9061 gttaaagcag accatgttcg gcaaaggaga aaacctggtt gccaccgact atcagatggt
     9121 ccggcccccg gatatccacc aggcccagtg cctgtaccag acgttcggtg ataagccggt
     9181 ctgccttact gggtgtgact tcaccggacg ggtgattgtg cgccagcacc acggcagcgg
     9241 cattgtggta cagggcgcgt ttaatcactt cccggggatg aacttccgta cggttgatgg
     9301 tgccggtgaa gagggtttca ccggcaatca gctgattctg gttgttcaga tacagcaccc
     9361 ggaactcttc ccgctccagt cccgccatgt tcagaatcag ccattcccgt gccgcacggg
     9421 tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc cagggtttgc agggcccgca
     9481 gaatgagact gcgctcgccg ggcgtcatct ctccgggcag aaaggaaagc tgctgcattg
     9541 tccctgcctc cattcagtca atgatgcgca taatggcgct gcattccgga tgctgcaggg
     9601 cgtaatcccg cagccggtaa tagtggaccg tcatggcata acactccgta cgacaggcat
     9661 gatgactgta cgtcatcaga caggcagcaa taccggcggc ttccgggctc atttcagcgc
     9721 ggttgccgtt catggcattg aacagtaccc atgtttcgtc atcatcgtca tccggttcgg
     9781 gtgccataaa tgccccgccg ttgttcaggg tgtacagatt ccagatacca ccgcagtagt
     9841 cttcgcaaag acggtccatc cagccgaaga cacggggctc cagggtcacc cactgtggaa
     9901 tgaggccaaa atgctgcggc cagaagctga tgcgctgttc atcagggacg ggagtggcaa
     9961 ccagctgagg taggttattc cctgatgcag tggttacgga aacagaaggt gtggtggaat
    10021 tatgcgaaac ggttgtcatg aggttattcc ttataaaaag taaatgaatg gaagaagccc
    10081 cggggcagga acagatgtga gtcaggactg cactttcagt gaaacggcat cagcgcatgc
    10141 tctccagcag cgtttctgcc atcacccaca atgcgcgatt gagcttaatg tcggtatcaa
    10201 tgctgtgaat ggcccgggta tggatacgtt ttccttttgc actgcgaccg gaaatcccgc
    10261 ctttcagcat attctcctgg atggtctgat aagcactcca caggtcctta ccgtaatcct
    10321 cccggcgtcg tggtgtcaga atgtcggcgg tggtgacggg ctgatgttcg tcaccataac
    10381 ggtaagtcag tgccgcctgt gccagcgcct ggcgtgccgg tggcggcagg accagcgact
    10441 gcatggcatc acgcttctcc tctatccggt caaaaacgcc caccacctcg taagcccctt
    10501 caatgactct gtccaccaca tttccccggt gtggaacacg caattctccc agagactgac
    10561 cgcagaccag tccattggta caaatggcac gaaaatatcc gggtaacatc tggtagctgg
    10621 atgaaccgtc atgggagttg agcagaataa tttcagggac atgctgaccg gttatctgtc
    10681 cggcccgccg cagacgcagc atgtgttttg tgtattcccg gcggctctgg tcgcgcacgc
    10741 gggtctggca ggcaaagaac ggctgaaagc cttcctgctg cagattttcc aggacggtga
    10801 tagtgggaat gtacgcatag tgttcactgc gagaggtatg tcggttttcc ccaaaaatac
    10861 tcggtacatg gcgcatcagt tcttcgtgtg tcagcggacg atcacgacgt atctggttcg
    10921 cataaccaaa acgactggct aatctcatga tttgctcctt atgaatgatt aagcgatata
    10981 aatgaaaaag ccgcgtctcc cggagaagac gcggcctgat gggtgaaatg aatgaggttt
    11041 attgtctgag aagcccttca ctggcgaacc acgtattaag ctgtattccg gcatcgccag
    11101 tgcaactgac cttcagcatt gcggataacc cgccggaaat atgttcactg gttgtttttg
    11161 tatcttcccc caaaattaaa taaattcatc ttaaccatca cccag
//
LOCUS       DAZFBR010000136        11060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFBR010000136 DAZFBR010000000
VERSION     DAZFBR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.136"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..1429)
                     /locus_tag="ACHJZ2_004176"
     CDS             complement(<1..1429)
                     /locus_tag="ACHJZ2_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO4570182.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEGAASFVPVETESDPM
                     NVAFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RVHRQHTENSAEKIPPVTAVIRREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTSLPQNANTAQNSEQ
                     PEPVKVTADEVKKIMQAANISQPDAN"
     gene            complement(1522..1713)
                     /gene="ydfD"
                     /locus_tag="ACHJZ2_004177"
     CDS             complement(1522..1713)
                     /gene="ydfD"
                     /locus_tag="ACHJZ2_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4570183.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            complement(1710..1898)
                     /gene="dicB"
                     /locus_tag="ACHJZ2_004178"
     CDS             complement(1710..1898)
                     /gene="dicB"
                     /locus_tag="ACHJZ2_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4570184.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            2457..2690
                     /locus_tag="ACHJZ2_004179"
     CDS             2457..2690
                     /locus_tag="ACHJZ2_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570185.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            complement(2668..3075)
                     /locus_tag="ACHJZ2_004180"
     CDS             complement(2668..3075)
                     /locus_tag="ACHJZ2_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570186.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(3098..3316)
                     /gene="ydfC"
                     /locus_tag="ACHJZ2_004181"
     CDS             complement(3098..3316)
                     /gene="ydfC"
                     /locus_tag="ACHJZ2_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO4570187.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(3389..3688)
                     /locus_tag="ACHJZ2_004182"
     CDS             complement(3389..3688)
                     /locus_tag="ACHJZ2_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570188.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            complement(3952..4359)
                     /locus_tag="ACHJZ2_004183"
     CDS             complement(3952..4359)
                     /locus_tag="ACHJZ2_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4570189.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4436..4663
                     /locus_tag="ACHJZ2_004184"
     CDS             4436..4663
                     /locus_tag="ACHJZ2_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4570190.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            4647..5198
                     /locus_tag="ACHJZ2_004185"
     CDS             4647..5198
                     /locus_tag="ACHJZ2_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570191.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKPDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGAYEYRCTTMG"
     gene            5170..6210
                     /locus_tag="ACHJZ2_004186"
     CDS             5170..6210
                     /locus_tag="ACHJZ2_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO4570192.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            6203..6664
                     /locus_tag="ACHJZ2_004187"
     CDS             6203..6664
                     /locus_tag="ACHJZ2_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4570193.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            6752..7432
                     /locus_tag="ACHJZ2_004188"
     CDS             6752..7432
                     /locus_tag="ACHJZ2_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4570194.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            7429..7593
                     /locus_tag="ACHJZ2_004189"
     CDS             7429..7593
                     /locus_tag="ACHJZ2_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570195.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            7598..8131
                     /locus_tag="ACHJZ2_004190"
                     /pseudo
     CDS             7598..8131
                     /locus_tag="ACHJZ2_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            8292..9050
                     /gene="paa"
                     /locus_tag="ACHJZ2_004191"
     CDS             8292..9050
                     /gene="paa"
                     /locus_tag="ACHJZ2_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO4570196.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            9386..9541
                     /gene="hokD"
                     /locus_tag="ACHJZ2_004192"
     CDS             9386..9541
                     /gene="hokD"
                     /locus_tag="ACHJZ2_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4570197.1"
                     /translation="MKQQKAMLIALIVICLIVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            9762..10019
                     /locus_tag="ACHJZ2_004193"
     CDS             9762..10019
                     /locus_tag="ACHJZ2_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570198.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            10089..10367
                     /locus_tag="ACHJZ2_004194"
     CDS             10089..10367
                     /locus_tag="ACHJZ2_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570199.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            10369..>11060
                     /locus_tag="ACHJZ2_004195"
     CDS             10369..>11060
                     /locus_tag="ACHJZ2_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4570200.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQ"
BASE COUNT         2892 a         2503 c         2818 g         2847 t
ORIGIN      
        1 gattggcgtc aggctggctg atattggctg cctgcataat tttctttact tcgtcagcgg
       61 taacttttac cggttctggt tgttcagaat tttgtgcggt atttgcattt tgcggtaagc
      121 ttgtgtatgt gccatttttt cgggcaaaat attcttcttt tgtgatttca gtagccccgg
      181 cagccagcgc cttatccaga ccagaaagtt tgtttgcgcg accgtatttt tcgccatcct
      241 tgtcggtgaa gaggaagtag aacggcccct cacgctctac agatggttcg acttccactt
      301 tgcattcggt tttttcgttg tccggaattg ccgtttccac tgcatcagtt tctggtactg
      361 gcgacgagag agtgtcagtt gcgctctgat ttgttccttc atcttcaaac acgccctttg
      421 tagtcaggta ttcagtaatg tatttgttca gtgccacagg gtctttgtga atgtcgatcg
      481 gacgttcacg gacaaggcca aaaatagtct ggcggtcgta gcgaagggca tcaggctgtt
      541 tgcgcattga tgccgagata cgcttccagt cttcgcggtc gttgtcgata acttcatttt
      601 ttgcccagcg atggatgctg ccgtcaatgt ttccggcatc cacatcacca ggccagagag
      661 cgtaggccag ttcgtcatcc agtgttttcc atgtctgctt atattcgcga cgaatgactg
      721 cagtgacagg ggggattttt tctgctgagt tttcagtgtg ctgtcggtgg actctggcgc
      781 gggcgagatc aacaacagac gtgtattttc cggtttcctt gcgttcacct tcgcgacgtt
      841 ttttccagat gcgcatttct gcctgaattt cgggccattt ggcaccaggc ttacatttat
      901 gcttaaccca cccgatggca tgcagcttaa gctccggata catggcgtta acttctggca
      961 ttttcatcaa cgcttcaacg atatgtccgt cgaatgttgc catgtcttcc tgcaacaatt
     1021 cctgcgcgct aatcaccata tcaacggtga tgttttcaca tgtgtcgaac ttaaccatga
     1081 cagcgttctg tacttcaggg gccagcttgt caaaagcgac gttcatcgga tcggattcag
     1141 tctcaaccgg gacaaaggaa gcagccccct catcccagcg gttttcctgc atatattcag
     1201 catcccagga atcgagggca gggcggggta taccgggttt atcctcgcaa acaagaaatt
     1261 tataagcgca gtcctgagca gccggataat gctccaggaa ttgccagtga aattttgcgc
     1321 gggcgcgacg ttcatcaccg gcttcaatgg cagtggctac agcgactgca ccttcttcct
     1381 ttattgcctg ttcgtccgga atggcggcgc aaataaagac tttactcatt ttgttttacc
     1441 tcattacaga tttaagggtg aacaaatccc tgccattgct ggcatataag aatgaaatcg
     1501 gatgtttatt acggaactgt tttaaagacc tgccgggatt tcgttattat cctggtgaat
     1561 aactttatcg acagggtaac agttaccggg aattttctgt tcggttgctg cagtcacaca
     1621 ctcctgcatt gtcctgtgaa cactgactgc aatatcaact ggctctccgg aaacaagaaa
     1681 aactgtcaga acaagtgcaa atgctgtatt cattgccagc atcctttttg tatcggacgt
     1741 aaacgggcca gcattgaaag aatgcatatt ttatttaata actcccgttc gtgttttctc
     1801 ttgttaatgg catcttcagt aaatacaggg ttactgatag tgacaccaat ttcaaaacaa
     1861 ccttcagacg tattaacgtt tggtaataac gtttccatta tcgcgtcctc aacaatgaat
     1921 tttgtgatgc ggtgcctggt gcctccaggt gacgttaacc agttaacaat taacgccgga
     1981 tacagagaat ccacccataa cactgttttt agctttaact gttccgcgtg cgcttagccg
     2041 cattcactgc atcacaaaat tcactttaaa aagggcggac atcagttcat gggcaaacag
     2101 atgccgccaa acgtcaccag aaaattgata acagagggcg ttgcagcggg gttgtcactt
     2161 aagcgtatgg tcaacctgac aacccggtgt cctcaacggg gaaggaataa ccccgccata
     2221 cttaccgctg cgccatttcg cggagtgcca caaccggaag cgcacggtcg acgaaaattt
     2281 aacgacaggc tatctatgaa ccagcaacct cgccgtgcgc tttcgcgtta tgctctgact
     2341 tttcagagaa atatcctttc agtaaactgt cagtgccgga tgttcacccg tgtccggcgc
     2401 acgcactcca cttcacccgt ggagaactcc ttaattacca accctcagga gggtgaatgt
     2461 taaaatcaac tcttattgct aaatgccttt atcaaaatcg catggtaagc agcatttcaa
     2521 taggcgagtc tgcagttaaa agtattttcg aagagtactt tcccgggcat gattttaata
     2581 aatggaatac caaattaccg ccagcagttt caacgcgtat tctgaaagca accgaaagag
     2641 caagtacaat tcgcgttaac tatttcatta aagatttgtg ggatctttga tatccacaga
     2701 gcctaaagta tgtgcatatg gatgtgctat tatgcgccct cgcagatttg catcattttc
     2761 taaattcact gaacgaaaca gggcatcaac aaggctctgt acaatgcaaa ggcaatcgaa
     2821 gactgtcgcc gtttctgttt tgattgatga aagaacatgg ccattcacgc aaacagaaat
     2881 tacccgttta ttaacatcgc tttcctgctt ttgattatca gaaccatata gcccagaaaa
     2941 agcattgcgc acattacgaa ccatattatc gatggtttct ttttctgcgg tactaaggtc
     3001 aagagtagcc agttgtgaac gaactatatt cgatgccatt tcctgtaatg gcgttggtaa
     3061 atctttaaat tccattatta gcctcgttgg ttagctatta acgtgggtat gtaatcattc
     3121 tggcaatgct taatgccgct gctttttcca gattggtgat atcctgctcc agagcggaca
     3181 gattttcagc ctgcttagcc ctggcttcat tggcccattt caggtcctgc accgccttaa
     3241 ttttctggtg catccactca taaagttcat catcggtata gtctggcgcg atgatgacgg
     3301 ggtctcgttt ctgcatgtcg gctccttgtg gttagcgttg cctgcttttt aaccacgtca
     3361 ggcgaggtgg tatcctctga ggggtctgtt actcgagagg aaattggtta tgaatacaat
     3421 caagttttct tgcccagaat gtggtggcta agtctttgac acatccttta agccgcaggg
     3481 ctctgacagt ttcgcgggag ccatctgcaa aaattgtggt caccttgtaa ctgaagatga
     3541 gtcctcgcag ttcgatgacg aaatcgttga caatatcttc ggtgcactca ccagagactt
     3601 tctgaagtaa aggcgcatac cgcttagtta ccgctctgat aactcttacc tgtccggcaa
     3661 tggcgctgat atcaatataa agcgccatcg ctgtttcttt gctgatccct ggacgccttc
     3721 cattctgatg tttgactcgc ccactgagaa atcctctgct tccccttaac gccgggtagc
     3781 ggaactgttt gctgagaaca ccgtgcggtg tcttgatgag tagaatttag aatagcctaa
     3841 gagttatggt caagcttttt gtgtagaaaa acctaagctt cttgatgtaa aaaacacaag
     3901 tatttgaaag tttgtgcttt ttattacaga gagttgcgaa aaaaaggggg gttatttatt
     3961 tgcgcttctt ttgcgagctt tgagtagttc ttcaaaaagt ttgttgaaat tctcaactcg
     4021 agcacgcatc tctgacaaca gagccttttg ctctgactca ggcagtgcgt cgaacagttg
     4081 aagcaactct ttttgatctt ctgtcagatt gactggctga ttatctggga tcggttcgcc
     4141 tggttgctta tcttcatctc caaaaagaag ccaagtcggc gagcactgaa gcgcctggct
     4201 cagtgcgaat aatctcttcc ccgctggctg tgtttcatct ctttcccatt gagaaattgt
     4261 tacgtgagcc actttgacca gcttacctaa tgcggcctga gacagtttta atttttttcg
     4321 cctgtataag aggcgagcac cgaaggtttc gtttttcata ttaggtaatt ctaatttttc
     4381 ttgacttagg tttctctacg atctagtttc cttaggaaaa tctaagggtt tcgatatgtt
     4441 gaaaattgat gctatagcgt tttttggcag caaaacaaag cttgccaatg tcgcaggagt
     4501 taggctggca agcgttgctg catgggggga actggttcct gaaggtcgcg cgatgcgcct
     4561 gcaagaggca tccggcgggg aacttcagta cgaccccaaa gtttatgacg aatatcgtaa
     4621 ggcaaagcgg gcggggcggt tgaacaatga aaatcaccac tgaacaggtt tgtgaggctc
     4681 tggatacctg ggtatgccga ccaggaatga cacaggagca ggcgacgata ttaatcacgg
     4741 aagcattctg ggctctgaaa gaacgcccga acatcgatgt tcaacgcgtc acgtttaatg
     4801 atggcgaggt tgatcaacgg gcgctgggcg ttaaccgggt gaagatattc gaacgctgga
     4861 aagctatcga caccagagat aagcctgaca aattcacggc gctgattccg gcaattatgg
     4921 aggctatccg gatcagcgat ttcagattgt attgtgaaat tactgacgga aaaagcatta
     4981 cgtacatgat cgccgggtta aacaaagaat atggcgatgt ggtggagtcc gggctgcttt
     5041 ttgcggatcc agttgttgtg gaacgtgaga ctgacgagct tatagaaaaa gctattgctt
     5101 tcaagcacgc gtatcgtcag caataccaat attactttgc agataaacaa atgtctgcca
     5161 ggggggcgta tgagtatcga tgcactacga tgggctaaaa aggtgaaaac cggcagttca
     5221 tccagtaagt cagtattgac ctggcttgct gatatgtgcg gtgccgattt gtgtgcatac
     5281 ccgtctgtat ctgcactggc agaagtaacg gaactgaaca aaaagactgt gcaggacagc
     5341 ttacgacacc tgatggagat tgggttaatt gttgataccg gtgagagaaa aggcagaaca
     5401 aagcaaattg tggtgtaccg acttatcggt gtagaagaaa gtgttgccga gcctgaatac
     5461 acccaaaaac gggagtcttt aaaggtgggt aaaattggtg ctgttaataa aaacagtacc
     5521 gaaaatggtt atgtttcagc acaaaacaga cccaaaaaca gaactcttag ctgcatggaa
     5581 aataaccaaa gacacccaaa ttttccatca aagacaccca aaaacggatc acggaaccca
     5641 aaggaaccca aagatctaaa ccccacacat aacgcacgcg agagtgctcc gaccagtgag
     5701 caggaagttt tgtcgttaca ggcagccccc cctgtattcc tgtatggcct gagcgaaccc
     5761 atcggaaaat tcccgatgag cgatagctgg tatccgtcac gggattttcg acgacgggct
     5821 gcgttgtggg ggatggcttt gccggagaca gaatttacac ctgctgaact tgccgccttc
     5881 cgggactact gggcagcgga ggggaaagtg tttacgcaga ttcagtggga gcagaaattc
     5941 gcccgtcacg taaatcacgt cagggcgcag gttaaaccag tcagcaaagg ggtaaaccat
     6001 gcagcagcac caggtggcac cgcatcacgg gcagttcagg aaattcgggc agcacgtgag
     6061 cagtgggaac gtgaaaacgg atttatcagc gacggaaacg gcctggaagc tgtgggaact
     6121 catgggggtg gtttattcga accgctggat ccagaagaac ggggccgcac cttcgaagct
     6181 ctggattgca cagattggcg cgatgactga gcagcaaatc cggcaggtct gccgccagtg
     6241 catggaccgc tgccgggcgg gtgaaacatg gcctccggac ctggctgagt ttgtggcact
     6301 gatttcgaaa agcggagcca atccattcgg tctgacggtg gatgctgtga tggaggagta
     6361 ccgccgctgg cgcaatgagt cctggcgata cgacggaagt gataagtacc cgtggtctca
     6421 gcctgtgctg tatcacattt gcctcgagat gcgttcaaag gggattgagc gccagatgac
     6481 cgaaggggaa ttaaaacggc ttgcagaacg gcaactgacg aaatgggcaa agcatgttag
     6541 taacggcctg agcgttccgc cagtccggcg acaactggcg gcccccaaac gcccgtcggg
     6601 gccaacgcca attgagttgc tgaaacagga atatgaacgc cggaaagcgg ctggttttgt
     6661 ttgagttgag aagtgatttt ttaccgggag gaaatttatg gagactgttt ttgacgcact
     6721 gaaagcgatg ggaaaagcca cgtcggtata gctggctgcg cgacttgata tcagtcgtga
     6781 agaagtactg aacgagctgt gggaactgaa aaaggctggc ttcgttgata aaagcgtata
     6841 cacctggcgt gtggctgata acaacgttca gcaggaacag ccagcgccag aagaaaccac
     6901 cacggcaaca gaagcgaaaa tctcagagtg cgatttaacc gcgacgattg aacaacgcgg
     6961 accacaaacg gcggatgaac tggctacgct gttcggtaca acatcccgca aagttgcttc
     7021 aacgctggca atggcaatca gcaaaggtcg tctgattcgc gtaaaccaga acggtaaaat
     7081 tcgttactgc ataccaggtg ataatttacc agcagagccg aaagttgaat cggtagcgga
     7141 aaccgatggt aaagcctttc ctcagccagc cggtgttgcg ttaccggtac agaaagatgc
     7201 aacacaggaa gatattaaaa cagaaactgt ggcggacatt gtgcagtcgc tgccatcgtt
     7261 tactgcaacg cgagaagatg atttgatttt gccatcgctg catatggcaa atcgcgaact
     7321 gcgtagggcg aagaatcatg tccagaagtg ggagcgagtc tgcgccgcgc tgcgggagct
     7381 gaacaagcac cgggatatgg ttgccgggat ttgtcggaag tccgggcaat gagcggatgg
     7441 tgcaggcctg aaatcatgat actaacaatg aaggtaaaat gcatcggcag tctgattggt
     7501 cgtagtgagg cggcggtcag gatgaaagcc caggttaagg gaataagcct gattctgcgg
     7561 ggtgattttc accagtcaac aaaatatccg tagcgcgata acggtcaaaa attatggcgc
     7621 tgacactttt gtgccactgg agatgactgt acctaagttc aggggagaag aacacgtccg
     7681 gtgggatggt cgggccagat ttaaagggca ggtcatggct ccagcctgta cgctggcaat
     7741 ggaggctgcc tggcaggaaa ttgatatggg aaccacgcca ctcagggatt tactgccggt
     7801 ccagagaata aattcctgtt acggttacac cactgtgatc ttgcaagtgc aggaaagtag
     7861 gtctacacgg taacgcgagt gcgtgtaact tttgatgtca ttcccgtaga aacaccggac
     7921 aaattttcgc tgacaggtca tgcagaaggt ataaatctgc agattatgga caattacgga
     7981 tatccggcaa gagccggaaa aagcatgccg cctctaattc tcagtggaag atggacttga
     8041 ttatactcat tgcattgtca gaaatagtta tccattaaag gctggctatt ccaaacagga
     8101 tgttgattac aaaaatgtaa tcaacatgta aggtttatac tcttcaatat gcgtataatt
     8161 ttccttattt tgttgacttt aaataacaag ctatgcacga ggtaaagtcg gataagttta
     8221 tctggatgta atatatatta tttgtagtgt ttataacttt atttcatgat aaccaataaa
     8281 aggagttttt tatgaggaac ataatggcag gttttttaat attcctgtct tctgctgctt
     8341 atgctgatat caatctgtat ggtcctggtg gcccgcatac agccttgctt gatgcagcca
     8401 aactttatgc cgaaaaaaca ggtattatag tgaacgttca ttacggccca cagaacaaat
     8461 ggaatgaaga tgccaaaaaa aatgcagata tcttgtttgg cgcatcagaa caatctgctc
     8521 tggctatcat tcgggaccat aaagacagct tcagtgaaaa agatattcag cctctttatc
     8581 tgcgaaaaag tattttactg gtaaagaaag gtaatcctaa aaatatccgg agtattgacg
     8641 acctgaccag acctgggatt ggcgtaattg ttaatgatgg tggtggtacc agtaatacat
     8701 caggcactgg cgtctgggaa gatattgccg gacgtaaagg gaatatagaa actgtcgccg
     8761 caatccgaaa aaatattatt ttatatgcgc ccaatagcgg aactgcacgt aaggctcttg
     8821 agaatcagcc tggagcagat gtctggataa cctgggctga ctgggcagcc agtaatccag
     8881 aaattggtga tgtcgtggaa atagcgccag actacgtgat atggcgtgat atgaacatta
     8941 cagtacgtca ggatgcaaat gatgaaaccc gtcgatttgc agaatggcta caaaccgatg
     9001 aagcggcgcc tgcattcaaa aaatatggct ggactaggaa aggcacttga catcctcgtc
     9061 cttcaggacg tggattcttt ttccggatgc cgcgccagcg gcatgtaggg gcagctcaca
     9121 aaacggaaaa aattgtacgc taagcctcgc caggtgaact gaattcattc cgatatggga
     9181 attcccatat cgggcgaaaa cggtttgctg taacggcaga gttaagtagg attgctgcgg
     9241 gtgcttgagg ctatctgcct cgggcatgaa caccaacggc agatagataa aagccccacc
     9301 cgactataaa tcgaagtgag gcccctatat gctcgtcaca tatagattgc ctcttacgga
     9361 ccgaaaggtc aaggagaagc aggctatgaa gcagcaaaag gcgatgttaa tcgccctgat
     9421 cgtcatctgt ttaatcgtca tagtgacggc actggtaacg aggaaagacc tctgcgaggt
     9481 acgaatccga accggccaga cggaggtcgc tgtcttcaca gcttacgaac ctgaggagta
     9541 agagaccagg cgggggagaa atccctcgcc acctctgatg tgtcaggcat cctcaacgca
     9601 cccgcactta acccgcttcg gcgggttttt gtttttattt tcaacgcgtt tgaagttccg
     9661 gacggcgccg gaatagaatc aaaaatactt aagtagcgcg cagggagaag agggatggac
     9721 cccgaacagg ggagtgctat ttatctggaa ggattctgtt gatgagaatc gaagaattac
     9781 gtgaaatttt tagtgaagat ggcctctata ctgtgcgcgt tgagaagggc gctattgtca
     9841 gccactgccg tattaaatgt ttacagtctc aacaaaggaa gagtggagct gcgttaattc
     9901 attttgtgga tgggcttgtg acggatggtt ttattttgcg tgcaaatgaa tttgtcacat
     9961 cgttgccgtc tctgaaagaa gctgggatta aggctggttt ttctgctttt gaagattagt
    10021 gaattcatct acaattcagc gcagggctga acccctgttg agtaacactg tgccaccgga
    10081 gaaagccgat ggcgcaaaat tccagactac acaattctga taattcagcc gtctttgcca
    10141 gcaggcacgg gcggcgttct catgcattca aatctgactg gttccggcac gccccatgca
    10201 ctgaagaaca ggccgaatgg ctgattcaga actaccgcag acgtgggtat gagtttagga
    10261 aagccctcag cctcgattat cgtcactgga taatctacgt caggctgccg tactccgagc
    10321 gcccaccgcg tccgtcccgc acattccagc aacgcatctg gaggtaacgt gcgggtatta
    10381 cttcgacctg ttccggtacc ggaacttggg gtggtggtcc ttaaaccagg ccgtgaatcc
    10441 atgcaggtat tccataaccc tcgagtgctt gtggagccgg agccgaaaag catgcgcggt
    10501 ctgccgtccg gcatcgtccc tgccgttcgc cagccgctgg cggaggataa atcattactg
    10561 ccgtttttca gcaatgagcg tgtgattcgt gctgttggtg gcgctggtgc actgtctgac
    10621 tggctgttgc gtcatgtcaa atcctgccag tggcctcatg gtgactatca tcacagtgaa
    10681 accgttattc accgttatgg taccggcgca atggtgttgt gctggcactg cgacaaccag
    10741 ctgcgtgacc agacatccga atcactcgag caacttgctc atcaaaacct gtcagcatgg
    10801 atgattgacg tcatcggtca cgcaataagc ggtacgcagg agcgtgaatt atctctggct
    10861 gaattatcct ggtgggcggt ccgcaatcag gtggcggacg cgctaccgga agcggtatta
    10921 cgtcgttcgc tggggttgcg tgcggaaaaa atccgctcaa tgtaccgtga aagcgacatc
    10981 gtaccgggag agcagaccgc caccatcata ctgaagcagc gcacaaaaaa tcttgcgccg
    11041 ctgcctcacg cccaccagca
//
LOCUS       DAZFBR010000137        10964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFBR010000137 DAZFBR010000000
VERSION     DAZFBR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.137"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            168..929
                     /gene="adiY"
                     /locus_tag="ACHJZ2_004196"
     CDS             168..929
                     /gene="adiY"
                     /locus_tag="ACHJZ2_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO4570201.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1066..2403
                     /gene="adiC"
                     /locus_tag="ACHJZ2_004197"
     CDS             1066..2403
                     /gene="adiC"
                     /locus_tag="ACHJZ2_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO4570202.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2507..4150
                     /gene="eptA"
                     /locus_tag="ACHJZ2_004198"
     CDS             2507..4150
                     /gene="eptA"
                     /locus_tag="ACHJZ2_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO4570203.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4147..4815
                     /gene="basR"
                     /locus_tag="ACHJZ2_004199"
     CDS             4147..4815
                     /gene="basR"
                     /locus_tag="ACHJZ2_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO4570204.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4816..5916
                     /gene="pmrB"
                     /locus_tag="ACHJZ2_004200"
     CDS             4816..5916
                     /gene="pmrB"
                     /locus_tag="ACHJZ2_004200"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO4570205.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5892..5981)
                     /gene="pmrR"
                     /locus_tag="ACHJZ2_004201"
     CDS             complement(5892..5981)
                     /gene="pmrR"
                     /locus_tag="ACHJZ2_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO4570206.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6093..7596)
                     /gene="proP"
                     /locus_tag="ACHJZ2_004202"
                     /pseudo
     CDS             complement(6093..7596)
                     /gene="proP"
                     /locus_tag="ACHJZ2_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
     gene            complement(7860..8738)
                     /gene="rdcB"
                     /locus_tag="ACHJZ2_004203"
     CDS             complement(7860..8738)
                     /gene="rdcB"
                     /locus_tag="ACHJZ2_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO4570207.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            complement(8735..10963)
                     /gene="crfC"
                     /locus_tag="ACHJZ2_004204"
     CDS             complement(8735..10963)
                     /gene="crfC"
                     /locus_tag="ACHJZ2_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO4570208.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2751 a         2864 c         2696 g         2653 t
ORIGIN      
        1 cctgtaaaaa aatatgtaca tgagaaattt ttataaaaat ttgtaatatt agtaaaactc
       61 gttattttta tgcatgttta tattcatcat acaattatat aaccatttcc cggtatcgct
      121 ttgctttagc gagaactggt cttttattcg cactcaggag tacatgtatg aggatttgca
      181 gcgaccaacc ttgtattgtt ttattgactg aaaaagatgt ctggataagg gttaatggga
      241 aagaacctat tagccttaaa gctaaccata tggcgttatt aaattgtgaa aataatatta
      301 tcgacgtctc ctctcttaac aacactttgg ttgctcatat tagtcacgac atcatcaaag
      361 attacctccg gtttctgaat aaagatctct cgcaaatacc agtatggcaa cgtagcgcta
      421 cgcccattct caccctgcca tgcctgacac cagacgtctt tcgcgttgcc gcgcaacaca
      481 gcatgatgcc cgcagaaact gagtcagaaa aggaacgaac acgcgcatta ttattcacgg
      541 tgctatcccg ttttctcgac agtaaaaaat tcctttcatt aatgatgtat atgttacgta
      601 attgtgtaag tgacagcgtt tatcaaatta ttgaaagcga tattcacaaa gactggaatc
      661 ttagtatggt agccagttgt ttatgtctta gcccaagtct gttaaagaaa aagctgaaaa
      721 gcgaaaacac cagttatagc caaataatca ccacctgccg catgcgttat gccgtaaatg
      781 aattaatgat ggacggtaaa aatatctctc aggtatcaca gtcctgcggc tacaacagta
      841 cgtcgtactt tatttctgtc tttaaagact tctacggtat gacaccgctg cattatgtta
      901 gtcagcacag agaacgcact gtcgcctgat ttttaacctt aacgaagagc tatattaata
      961 acggcatcag cgataacccg gtcgataata attcaactat cgaatgcagg cgtatgatat
     1021 gacgtaattt attgtcacga agctcgcctt cgcaggagtt taattatgtc ttcggatgct
     1081 gatgctcaca aagtgggctt aatccccgtc accctgatgg tgtcggggaa tattatgggg
     1141 tcaggtgttt ttctgttacc tgcaaacctg gcctccaccg gcgggattgc catttatgga
     1201 tggttggtga cgattatcgg tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc
     1261 ctcgacccaa gtcctggtgg ttcttacgct tacgcccgcc gctgctttgg cccgtttctc
     1321 ggttatcaaa ccaacgtcct ctactggctg gcctgctgga tcggcaatat cgccatggtg
     1381 gtcattggcg taggatattt aagttacttc ttcccgattc tgaaagatcc attggtatta
     1441 accatcacct gcgtcgtggt gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa
     1501 atgatcaccc gtgtgcaggc cgttgccacc gtgctggcgc tgatccccat cgtcgggatt
     1561 gccgtatttg gctggttctg gtttcgtggt gaaacctata tggcggcatg gaacgtcagc
     1621 ggcctgggca ccttcggtgc aattcaaagt acccttaacg ttacgctgtg gtcgttcatc
     1681 ggtgtggaaa gtgcctccgt tgccgcaggt gtggtgaaaa acccgaaacg caacgtccct
     1741 atagccacca ttggtggggt attgattgcc gccgtttgct atgtgctctc caccaccgcg
     1801 attatgggga tgatccctaa tgccgcactg cgcgtttctg cctcgccatt cggtgatgcc
     1861 gcacggatgg cgttgggtga caccgccggg gccatcgttt ccttctgcgc agctgcgggt
     1921 tgcttaggtt cactgggcgg ctggacattg ctggcgggtc aaacggcgaa agcggctgcc
     1981 gatgacggac tgttcccacc gatttttgcc cgtgtaaata aagcgggtac gccagtggcg
     2041 gggctgatta tcgtcggtat tttgatgacc atcttccagc tcagcagcat ttcaccaaac
     2101 gcgaccaaag aattcggtct ggtttcttcc gtgtcggtca tctttacact ggtgccatat
     2161 ctttacacct gtgcggcgtt actgctgctc ggacacggtc actttggtaa agcacgcccg
     2221 gcatatctgg cggttactac cattgccttc ctctactgca tctgggccgt ggtggggtcc
     2281 ggggcgaaag aggttatgtg gtcatttgtc accctgatgg tcatcaccgc catgtatgcg
     2341 ctgaattaca accggctgca taaaaacccg tatcccttag atgcaccaat aagcaaagat
     2401 taattcccct taatccagca aagataaaag ccaaccttaa gaacttaagg ttggcttaat
     2461 tttgctttgc gagcatatgc gcactttgtt cgatggaaac accgtgatgt tgaagcgcct
     2521 actaaaaaga ccctctttga atttactcgc ctggctattg ttggccgctt tttatatctc
     2581 tatctgcctg aatattgcct tttttaaaca ggtgttgcag gcgctgccgc tggactcgct
     2641 gcataacgta ctggttttct tgtcgatgcc ggtcgtagcc ttcagcgtga ttaatattgt
     2701 cctgacacta agctctttct tatggcttaa tcgaccgctg gcctgcctgt ttattctggt
     2761 tggcgcggct gcacaatatt tcataatgac ttacggcatc gtcatcgacc gctcgatgat
     2821 tgccaatatt attgatacca ctccggcaga aagctatgcg ctgatgacac cgcaaatgtt
     2881 attaacgctg ggattcagcg gcgtgcttgc tgcgctgatt gcctgctgga ttaaaatcaa
     2941 acctgccacc tcgcgtctgc gcagtgttct tttccgtgga gccaatattc tggtttctgt
     3001 actgctgatt ttgctggtcg ccgcactatt ttataaagac tacgcctcgt tgttccgcaa
     3061 caacaaagag ctggtgaagt ccttaagccc ctctaacagc attgttgcca gctggtcatg
     3121 gtactcccat cagcgactgg caaatctgcc gctggtgcga attggtgaag acgcgcaccg
     3181 caacccatta atgcagaacg aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac
     3241 ctcgcgggcg gagaacttct ccctcaacgg ctacccgcgt gaaactaacc cgcggttggc
     3301 gaaagataac gtggtctatt tccctaatac cgcatcttgc ggcacggcaa cggcagtttc
     3361 agtaccgtgc atgttctcgg atatgccgcg agagcactac aaagaagagc tggcacagca
     3421 ccaggaaggc gtgctggata tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa
     3481 cgatggcggc tgtaaaggtg cctgcgaccg cgtgcctcac cagaacgtca ccgcgctgaa
     3541 tctgcctgat cagtgcatca acggcgaatg ctatgacgaa gtgctgttcc acgggctgga
     3601 agagtacatc aataacctgc agggtgatgg cgtgattgtc ttacacacca tcggcagcca
     3661 cggtccgacc tattacaacc gctatccgcc gcagttcagg aaatttaccc caacctgcga
     3721 caccaatgag atccagacct gtaccaaaga gcaactggtg aacacttacg acaacacgct
     3781 ggtttacgtc gactatattg ttgataaagc gattaatctg ctgaaagaac atcaggataa
     3841 atttaccacc agcctggttt atctttctga ccacggtgaa tcgttaggtg aaaatggcat
     3901 ctatctgcac ggtctgcctt atgccatcgc cccggatagc caaaaacagg tgccgatgct
     3961 gctgtggctg tcggaggatt atcaaaaacg gtatcaggtt gaccagaact gcctgcaaaa
     4021 acaggcgcaa acgcaacact attcacaaga caatttattc tccacgctat tgggattaac
     4081 tggcgttgag acgaagtatt accaggctgc ggatgatatt ctgcaaactt gcaggagagt
     4141 gagtgaatga aaattctgat tgttgaagac gatacgctgt tattgcaggg actgattctg
     4201 gcggcgcaaa ccgaaggcta cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa
     4261 agccttgagg caggtcatta cagcctggtg gtactggatt tagggttacc cgacgaagat
     4321 ggactgcatt ttctcgcccg tatccggcag aaaaaatata ccctgccggt actgatcctc
     4381 accgctcgcg atacgctgac cgacaaaatc gccgggctgg atgtcggtgc cgacgactat
     4441 ctggtgaagc cttttgcgct ggaagagtta catgcccgta tccgcgccct gctacgacgc
     4501 cataataatc agggcgaaag tgagctgatt gttggcaatc tgacgctgaa catgggtcgc
     4561 cgtcaggtat ggatgggcgg tgaagagttg attctgacgc ccaaagaata tgctctgctg
     4621 tcacggttaa tgctcaaagc aggcagtccg gtgcatcggg aaattctcta caacgacatc
     4681 tataactggg acaatgaacc ctcgaccaac accctggaag tgcatatcca caatctgcgc
     4741 gacaaagtgg gcaaagcccg tatccgcacc gtgcgcggct ttggctatat gcttgtcgcg
     4801 aatgaggaaa actaattgaa tctgatgcgt tttctgcgcc gaccaatatc gctgcgccaa
     4861 cggctgatat tgaccatcgg ggccattttg ttggtgtttg agctgatcag cgtcttctgg
     4921 ctatggcatg aaagtaccga gcagattcag ctgtttgagc aggcgctacg cgacaatcgc
     4981 aacaacgatc gccacatcat gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc
     5041 ggcgtcttta tggtcagcct gacgctattt atctgctatc aggcggtacg ccgcatcacc
     5101 cgcccgctgg cggagctgca aaaagagctg gaagcgcgca ccgccgacaa cttaacgccc
     5161 attgccattc acagcgccac cctcgaaatc gaagcggtgg tttcggcgtt gaacgatctg
     5221 gtcagtcgcc tgaccagcac gctggataac gaaaggttat ttaccgctga cgtcgcgcac
     5281 gaactgcgaa cgccactggc gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat
     5341 cacattgatg tagcaccgtt agtggcacgg cttgatcaga tgatggagag cgtctcccag
     5401 ctgctgcaac tggcgcgtgc cggacagtca ttttcttccg gtaattatca acatgtaaaa
     5461 ctgctggaag atgtcattct cccctcgtat gacgaactca gtaccatgct cgaccagcga
     5521 cagcaaaccc tgctattgcc agagagcgcc gccgacatca ccgttcaggg tgatgcaacc
     5581 ctgctgcgga tgttattgcg aaacctggta gaaaacgccc atcgttacag cccgcaaggc
     5641 agcaacatta tgattaagct gcaagaagat ggcggagcgg tcatggccgt tgaagatgaa
     5701 ggaccgggta ttgatgagag taaatgcggg gagttgagta aagcgtttgt acgtatggac
     5761 agccgttatg gcgggattgg tctggggtta agtattgtca gccgcattac acagttgcat
     5821 cacgggcagt ttttcctgca aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg
     5881 ctgaagaaag atcagaacgt ggcaaaccag atataaagaa agctgctgac caccagcacg
     5941 ctgaacacgg tagttaaact ttcataaaca cggtttttca ttgcactctc ctccgttaac
     6001 ctggaggaga gtatgcgcgt cagagattaa gtgaacctta agagttcaat tggcctgata
     6061 agacagcgtt acatcaggcc atccgtttca gcttattcat caattcgcgg atgttgctgc
     6121 accaggcggg tacgtttcgc ctgcaaatcg gcaatctcgt ggtcaatatc atcgattttc
     6181 tgctcgatat tatcgtaatg ctcgacgaga atttccttcg cttcctgtat atctgacgcc
     6241 gccggtgttg cacctttcaa cggacgattt gccgtctctt tcatggttac gccggtgatt
     6301 aaaccaacca ccgccactac catcaggtaa taggctggca tcatcagatt ctgcgagctt
     6361 tcgaccagcc aggccgccag tgttggcgtc agaccggcaa ccagcaccga aatattaaat
     6421 gccgccgcca gcgcgctgta acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc
     6481 ataacgcccg taaagcagtt aaggatcacc gccagcatca gtaacccggc aaaaatcagg
     6541 ccgatgacgt tactgttaat cagaataaac gccgggatcg ccaacacaaa cagtgcaaca
     6601 ctaccaagta gcacaaacgg acgacggcca aaacggtcac tcagcaagcc catcaccggc
     6661 tggacaaaca gcataccgat cataatggcg ataataatca gcaccccgtg gtcttcggag
     6721 taatgcaggt tatgcgacaa ataactcggc atataggtca gcaacatgta gtaagtcacg
     6781 ttggtggcaa ttaccagacc aatacatgtc aacaggctgc gccagtattt agtggcaatc
     6841 tctttaaacg agactttcgg gccatcctgc aaaccttcgc ggtcgccctg ttccagttta
     6901 tcaacatgct gctggaacgc cggagtttct tccagcgcat ggcgcaggta aagcccgata
     6961 atccctaacg gcagagcaat aaagaacgga atacgccagc cccagtcgag gaagttcgct
     7021 tcgccgacaa tggtcgaaat taacaccacc acgcccgcac ccagcacaaa cccggcaata
     7081 gaaccgaagt ccagccagct gcccataaag ccacgtttac ggtcagggga gtattccgca
     7141 acaaatatcg acgccccggt atattcaccg ccgaccgaga aaccttgtgc catcttacag
     7201 atcaacagca gaatcggtgc ccaaatacca atcgtgtcgt aggacggtat taagccaata
     7261 cagaacgtac tgatcgacat aatcacaata gtgatagcga ggatcttctg gcgaccatat
     7321 ttatcgccca acataccaaa gaagagtccg ccaagcggtc gaatcagaaa gggaacggag
     7381 aaagtggcaa gtgcagcaac catctgcacg ctggggtcag cccccgggga aaaaaacttt
     7441 acctaatgcg taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc
     7501 cagtgatgct gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg
     7561 aagggtaatc ggttttactt ttttcctttt cagcatagct ttcctcgcag agttggccca
     7621 tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg
     7681 atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca
     7741 atcaaaccag ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagcgttta
     7801 aatttgtgat caacttcaca catggatttc gtttaggact cattgatgta actgattatt
     7861 tacaccaaat ttacagagaa gatttcctcg agcattgccc ccgccagacg ctgcgctgaa
     7921 cagagtcgtg gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg
     7981 ggttgtttat ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg
     8041 cccgtcggaa cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggggcc
     8101 gcattgatac tctctgccag atgttgtgtc gctttcacgc gcaaatcacc cagcaacaac
     8161 gccaccgctt gcgggcttcc cagacgacag gcatcgccaa atgcccccca acgcaactct
     8221 tcgagaacga taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag
     8281 cgggtaaaaa tttgctcgct ctgttcaccc gcctgcacca gtaggcctcg ttgcgttaac
     8341 gccttttcct tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg
     8401 gggagctccg ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc
     8461 tgcgcgatct gcgtcagtgc cagatgaccg ggatccagca tcgacgccag tttttccacc
     8521 aatgtcagac gcagactaag cggcgcattt aacatcccgc cgttcgtcca tgtctgaagc
     8581 tgcacgcgcg caagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttgt
     8641 ggaaggcggg catcatcgcc ctgcgccaga tccactaaaa agcggggata aaccgactcc
     8701 agcacgcgac cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg
     8761 tttgaatatc atcgcgtaac agctgagcgt cggtgtaaat ccagccgtga gtcatcacag
     8821 tctgctgcaa ttgctgttgc atcagcctga ccaccgattc attttgttga cgcagagcca
     8881 gactttcgcg taaacgcgtc tgtaattccg tcaaacatga agcgaactca gcgaaaaaag
     8941 tattcatgcc tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt
     9001 ggcaataaag atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt
     9061 ctgtcgggga tttctcattt ccccgtcccc agtcaggctg attcaaccag cgcgaaaaag
     9121 tctcacgcac aacgcctaat cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga
     9181 cttcactgaa cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat
     9241 gaaacccagg acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc
     9301 gaataacatc ggttaacacc cggcaaaggg tggattcaag ctcggcaaaa cgcagcgtaa
     9361 agtcgcgact gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct
     9421 cgcttaacgg ctccgaatca tcctgaaaca aggcggctaa ctgcaccttc agcgcatctt
     9481 gctgttgacg cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct
     9541 cgccgctcac ctgcgcctga ttgagttgca atagctgcaa actttcttcg atctggtgga
     9601 tattttgccg caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt
     9661 cccgcgcctg ctgcgcgtaa ttcaacagtt tatgcgcggc agatcgcaga gcatacaacg
     9721 aggcgttagc gtaagcggca tgaagcaaca tctgaattgg ctgggcgaac agcgaatctt
     9781 cccacaactg atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc
     9841 agcgcctgcc gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcggtg
     9901 gtaacttacc gttgttggct aactcatggc gcgcccgatt tgccaggtag ccccacatcg
     9961 acgacaccgg aaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca
    10021 gtgcccgcac ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca
    10081 gcacatacag cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg
    10141 agatcgattt cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct
    10201 gctggttaag cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca
    10261 gtaacgtcaa ttgaccggga taactttcca gccccgccag atggacaaac tccacttcaa
    10321 tcacgggaat atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct
    10381 tcgccagtcg caccaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca
    10441 gataatattt ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt
    10501 tatctatttc cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta
    10561 atttttgaat taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt
    10621 ggccgggcgt atggcgaata agcgtcggca gcgcagtcat tgggcgattg cgattaggca
    10681 gtacctccgt accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga
    10741 caatcgccag caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt
    10801 gacgctcggc gatattaggc tgactccagg gtaaagccag ttgtggcgca tcgtctccgg
    10861 gtacagagag aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacagcc
    10921 gttcagcctc ctgacttaac tcatacaggg tctgtgtgta cata
//
LOCUS       DAZFBR010000138        10813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFBR010000138 DAZFBR010000000
VERSION     DAZFBR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.138"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..62)
                     /locus_tag="ACHJZ2_004205"
     CDS             complement(<1..62)
                     /locus_tag="ACHJZ2_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570209.1"
                     /translation="MALYTIGEVALLCDINPVTLR"
     gene            284..1969
                     /gene="btsS"
                     /locus_tag="ACHJZ2_004206"
     CDS             284..1969
                     /gene="btsS"
                     /locus_tag="ACHJZ2_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO4570210.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            1966..2685
                     /gene="btsR"
                     /locus_tag="ACHJZ2_004207"
                     /gene_synonym="yehT"
     CDS             1966..2685
                     /gene="btsR"
                     /locus_tag="ACHJZ2_004207"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO4570211.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            2732..3202
                     /gene="yehS"
                     /locus_tag="ACHJZ2_004208"
     CDS             2732..3202
                     /gene="yehS"
                     /locus_tag="ACHJZ2_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO4570212.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3244..3705)
                     /gene="yehR"
                     /locus_tag="ACHJZ2_004209"
     CDS             complement(3244..3705)
                     /gene="yehR"
                     /locus_tag="ACHJZ2_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO4570213.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(3830..5833)
                     /gene="yehQ"
                     /locus_tag="ACHJZ2_004210"
     CDS             complement(3830..5833)
                     /gene="yehQ"
                     /locus_tag="ACHJZ2_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO4570214.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPLLQQQG"
     gene            complement(5830..6966)
                     /gene="yehP"
                     /locus_tag="ACHJZ2_004211"
     CDS             complement(5830..6966)
                     /gene="yehP"
                     /locus_tag="ACHJZ2_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4570215.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(6959..9238)
                     /gene="yehM"
                     /locus_tag="ACHJZ2_004212"
                     /pseudo
     CDS             complement(6959..9238)
                     /gene="yehM"
                     /locus_tag="ACHJZ2_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(9249..10337)
                     /gene="yehL"
                     /locus_tag="ACHJZ2_004213"
     CDS             complement(9249..10337)
                     /gene="yehL"
                     /locus_tag="ACHJZ2_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO4570216.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         2562 a         2786 c         2872 g         2593 t
ORIGIN      
        1 cgtaacgtga cagggttaat atcacaaagc aacgccactt caccaattgt gtaaagcgcc
       61 atcgtctcac ccttgctcgc gaggtcccgg tttaacttta gacgcagttt tgcgaaccag
      121 gtagttttgc ccgttttttg tgcatttata gggtgatttt atttttgcca ggcgattttg
      181 agtgatcgta ctcacgaatt ctcatttttc tgcaagagtt caaagaaagt taaacgcagg
      241 caatgtatgt tacgcgtttt aaagggatgt gtggtttgcg ggtatgtacg attttaatct
      301 ggtgttgctg ctgcttcagc agatgtgcgt ttttttagtc attgcgtggt taatgagtaa
      361 aacgccatta ttcataccgt taatgcaggt cacggttcgt ctgccgcata aatttctctg
      421 ctacatcgtc ttttccatct tctgcatcat gggcacctgg tttgggttgc acattgacga
      481 ttctattgcc aatacccgtg cgataggcgc ggtaatgggc ggcttactcg gcggtccggt
      541 cgtcggtggg ctggttggcc tgaccggcgg gttacatcga tattcgatgg ggggcatgac
      601 cgcgctaagt tgcatgatct cgaccatcgt tgaaggattg ctcggcggcc tggtacacag
      661 catcctgatc cgtcgcgggc gcactgataa agtctttaac cccattaccg ctggtgccgt
      721 cacgttcgtc gctgaaatgg tgcaaatgct gatcatcctg gcgatcgccc gaccttatga
      781 agatgcggtg cgtctggtga gtaatattgc tgcgccaatg atggtcacca ataccgtcgg
      841 cgcggcgctg tttatgcgta tattgctcga taaacgcgcg atgtttgaaa aatacacttc
      901 ggctttttct gccactgcgc tgaaagtggc agcctcgacg gaaggcattt tgcgccaggg
      961 gtttaacgaa gtgaacagca tgaaagtggc tcaggtgctg tatctggagc tggatattgg
     1021 tgcagtcgcg attaccgatc gagagaaatt gctggccttt accggaattg gtgacgacca
     1081 ccatttaccc ggcaaaccga tttcttcaac ttatacttta aaagcgattg aaaccggtga
     1141 agtggtctac gctgatggca acgaagtacc ttatcgttgc tctttgcatc cgcaatgcaa
     1201 actggggtcg acgctggtaa ttccgttgcg tggtgaaaat cagcgggtga tgggcaccat
     1261 caaattgtat gaagccaaaa accgtttatt tagttcaatc aaccgcacgc tgggcgaggg
     1321 gattgcgcaa ctgctttcgg cgcagatcct cgccgggcaa tatgagcggc aaaaagcgat
     1381 gctcacccag tcagagatca aactgcttca cgcccaggtg aacccccatt ttttgtttaa
     1441 tgcgcttaac accattaaag cggtgatccg tcgcgacagc gaacaggcca gccagctggt
     1501 gcagtatctt tccacttttt tccgcaaaaa cttaaagcgg ccttcggagt ttgttactct
     1561 cgccgacgaa attgaacatg tgaatgctta tctgcaaatt gaaaaggcgc gcttccagtc
     1621 gcggttgcag gtcaacattg ctattccgca agaattatcc cagcagcaat tgcccgcgtt
     1681 taccctgcaa ccgatagtgg aaaacgccat taaacatggg acatcacaac tgctggatac
     1741 agggcgagtg gcaatcagcg tccgacgtga ggggcaacat ttgatgctgg agatcgaaga
     1801 caatgccggt ttgtatcaac cggtaaccaa tgccagtggg ctggggatga atctggtgga
     1861 taagcgttta cgtgaacggt ttggcgatga ctatgggata agcgtcgcct gtgagcctga
     1921 tagttacacc cgaataacgt tacgactacc atggagggac gaggcatgat taaagtctta
     1981 attgtcgatg atgaaccgtt agcacgggag aacctgcgcg tatttttgca ggagcagagc
     2041 gatattgaaa tcgttggtga gtgttcaaac gccgtggaag ggatcggcgc ggtgcataaa
     2101 ctgcgcccgg atgtgctgtt tctcgatatc cagatgccgc gcatcagtgg tctggaaatg
     2161 gtggggatgc tcgacccgga acatcgtccg tatattgttt ttctcaccgc gtttgacgaa
     2221 tacgctatta aagcctttga agaacatgcc tttgactatc tgctgaagcc aattgatgaa
     2281 gcgcgactgg agaaaacgct ggcgcgattg cgtcaggagc gcagcaagca ggatgtttcg
     2341 ctgttaccgg aaaatcaaca ggcgctgaaa tttatccctt gtacggggca tagtcggatt
     2401 tatttgctgc aaatgaaaga tgtggcattt gtcagcagtc ggatgagcgg tgtctacgtt
     2461 accagccacg aagggaaaga gggctttacc gaattgacat tacgtaccct ggaaagtcgt
     2521 acaccactac tgcgctgcca tcgtcagtat ctggttaacc tcgcgcattt acaggagatt
     2581 cgtctggaag ataacggtca ggccgagttg attttgcgta atggcttaac cgtgccggtc
     2641 agccgccgtt atctgaaaag cttaaaagag gcgattggcc tgtaaaagac tgctaaaatg
     2701 gctttttgcc tcatcaacac ctgaaggcct catgctaagt aacgatattc tgcgcagcgt
     2761 gcgctacatt ttgaaagcca ataataatga cctggtgcgt atactggcgc tgggtaatgt
     2821 cgaagccacc gcggaacaga tcgccgtctg gctacgtaaa gaagacgaag agggttttca
     2881 gcgttgtccg gacattgttt tgtcgtcatt cctcaatggc ctgatttatg aaaaacgcgg
     2941 caaggatgag tctgctccgg cactggagcc ggaacgtcgc attaataaca acatcgtgct
     3001 gaaaaaatta cgcatcgcgt tttcgctgaa aaccgatgac attctggcga tcctcaccga
     3061 acagcagttc cgcgtttcga tgccggaaat tacggcgatg atgcgtgcac cggatcataa
     3121 aaacttccgc gaatgcggcg atcaattttt acgttatttt ctgcgtggac tggcagcgcg
     3181 ccagcatgtg aagaaaagct aagacgggta tggcggccat gcgcaaacat ggccgccgac
     3241 agattatttc acttctttaa aaccagcggc tttcatcacc agttccattt gcgccatagt
     3301 gatacctttt ttggcatctt cagcagaaac gttgattcct gaaataccct gcagggcttt
     3361 aaaatccact ttttccatat cgatagtcac gttttcctgc gcgtaggtat cggtataggt
     3421 taatttttct tcaacacccg cgatgttttt gtatttggcg cttaacggct caagtgtctt
     3481 ggcagcatct tctttggtgg tagcaccaat ggaggcaaat tgaattttgg tttcagaaga
     3541 ttgcttaagc accttgtcac ctttgtagac ataggtaatg gcaatttcag tgccgttcag
     3601 attggcgctg aatttcttcg attcttcttt gtcaccgcag ccagcaagag aaaaaaccag
     3661 aacagatgca acaacaaggg aaaacagctt attgaaagcc ttcatgtaaa actccatttt
     3721 atttaatcaa ggaactggtg actctcacca ggggctatat aggatatgcc taataccgtg
     3781 gcgtgagcag tccggaactg gagtagaact cttagtaaaa agcactattt catccttgtt
     3841 gctgaagcag ggggaataat tgttcgcaca gcaaaacacc gttattcatt gcttctaccc
     3901 gtgcctcgct ttctgtatta cgaaattgtc ccaatacatg tgccagccga taaaaaccca
     3961 ccgcggtgag gtcattcgcc agcaactctg cctgaccaat agcactctgt tcctgatagc
     4021 gccagccgtt atggagcagt tgaataagta atgcctggca gcgcatcagc aactgatgag
     4081 cagtagacgg cacaggcaga acgctggcag aaggtagtga tgccaccaca ggcgcagttt
     4141 ctgcgtccag cgcccaggca cgagtctttg tcatcattac ctgtggttcc agtgtcaatt
     4201 gcccatcaac aaaactgaca aagccagaaa ccagacacac ggggtcgtct gtttgttgca
     4261 aaagcgccgc catgcgttca acggcataag gcgcgctggc tgaggctggc agtgataacg
     4321 tcagcagatt atcttcacct tcgccgctga ttacctgcgc atccagcgtc tggcggctgc
     4381 tatcccaacc gagcgaaata cactcagcga ccggcagaat aaataagtta tcgacctgat
     4441 taagaggccg tatgcaggag gggggacgct ggcgtaaata ttcccgcaaa gccacaatgc
     4501 ccggctggcg taacggcgcg ctcaacattc gccaggcatc aggcgacagc ggcacaacgc
     4561 tgcttaagcg gttgcgggta gctaacagca gctcgccatc ggcactgcgt tttgctgctt
     4621 gtgaaacaat ttgcccaccc gccagtgcgc cagcctgaaa actaaacagc cgacgcgtag
     4681 ctgccggtga gttttcctgt tcacttcgcg gccaactgcg cgaaaggtgc aaaatactgc
     4741 cggtgtcggg atcggtaaac cagatgcgta aaccataatg ctcaatatcc tgccagcaac
     4801 gcatacctaa agacaccagc cgcagatgat caagctttgc ttctccggca atgccagagc
     4861 caacgaccgt gcgccacggc acaggaggaa cttcaccaac actgtcgcgc cgggccatct
     4921 cttgtgcgca atttaatcga ctgtttaatg ccgcaagctg acgtaagcat tctcctgcat
     4981 gatagtggct ggcgcgggcg tggaaggcat caacgctggc gcgcagttgc cgtagcgatt
     5041 cactcaccca gcgccagttg cagcgttccg ccgcttgctg cgcgcggctg aaagcggcct
     5101 cgtagtgaat aagcggctgg ctgatgccgc caagccataa tgcctggctt aattgctgaa
     5161 catattgacg acacgcgttg ccttcttcgc tggcaaacgg gtcgtcagat gatgtgacgt
     5221 gttcgctgcg catctgccag attaaatgag taaattccgc ttgctgagtt ttggcctcga
     5281 cgaaggcctg cacagccagt acgacatgtt cgcaaagtgt gccttcaata caatcacagc
     5341 gggcgaagcg aatgctgctg cgggaataaa aacgcacatc actcatcggt aagcgggctg
     5401 agggaatttc gccaggcgca cagaacaact caatggtgat gcctttagcg accagcacct
     5461 gtgcacgttt gcgggtggca tcgggcaggg tagccagttc ttccagccag attgcaggct
     5521 gccactcttc ttctttttcc gtgggccggg tggtggcaca aagtcgctga taacttaaca
     5581 ccagcatcac gcgatgacgg cacatgccgc tagccccgca ggtgcactga gcctctttca
     5641 gtgcctggcc gttcgccagc tgggtacgga caccgtcact gaaggtggcg attaaagcgc
     5701 cgttctcatg gctgatttcc gggacgttgc cattttccag ttccttaagg ctgcgcttaa
     5761 caaaaccggc attgcttaac gccgtcaggg cctgcggcgt gagttctaat aattccggac
     5821 gtagtgaatt catgactgca aattctccgc aagccaggta gccagttcac ccggtgtcat
     5881 tgcggctatt tgtgcgccaa cattaaccag cgcctgggcc atatcgcggt catagcaagg
     5941 cgttgcggtg ctgtcgagcg ctgccagccc cagcactttg atgccgctct ggacacactt
     6001 tttcacctga tgcgtcagca atgatgatga acccccttca taaaaatcac tcacgaggat
     6061 aatgacgctt tttgctggtt gttcaataag ttgccgccca tactccacgg cactggcgat
     6121 attggtcccg ccgcccaact gtactttcat taataactct accggatcgg caacgtctgc
     6181 cgtgagatca acgacactgg tgtcaaacgc caccagatgg gtacgaatgc cgggtaactg
     6241 ccacaaacag gccgccatta ccgcagagtg gatcaccgaa tcgaccatcg atccgcttag
     6301 atcaaccagt aagaccagtt gccattgttc actgtggcgc ttaattcggc tgttaaagcg
     6361 gggggattcg atatacaact tgccgtgttg cgggtgccag tgttgcagat tggcgcgaag
     6421 agtacttttg aaatcaaagt ctcgcgccag tggaatagat gagcggcgac ggcgatcgcg
     6481 gacaccagaa aaagcctgac gaacttcctt tgccagtcga gccataattt cttcaacaac
     6541 ctggcgcact atccggcggg cggcagccag tacttcgggg ttcatcagat gtttggtgtg
     6601 caaaacggcg cgtagcaggc tttcagaagg ctgcatacgt tccagcacgt cgagattcgt
     6661 caccacatct tcaatgccgt agcgcagtac ggcatcgctt tccagccgct caatcacctg
     6721 ttgcggaaac agcgtgtgaa tactgttgat ccactcaggg gtggtgagat ttgagccacc
     6781 taatccaccg gagcgttcac cacgctggag ccgttcagga tcgcgcccat acagccactc
     6841 cagcgcgtgg tctatctgcc gggcgttgtc atccagccca caaagcgtcg tttctgccgc
     6901 ttcgccaaga attaatcgcc agcgttgtag ctcacgggtg gtcagaagat cgttcagttc
     6961 agacatggaa aactccccag ttttgcagcg atgccagtgc ctgctgttcg agttgttgat
     7021 gatgtgcaat ggcttgtggt gggcaatgca acggcatttg cagcgccgaa acgggaagtt
     7081 gcgccagttg ataatgttca agcacctgat gcgccagtgt cccgcgttct cgtggcggta
     7141 gccaggccat cgccgcgcgt aaatcgggca gggcattgat aaaatcggca tcacttggtt
     7201 gatttaaatg actgctgaaa ccggcgataa atgccggctg acaggccagt tgatggcggg
     7261 ccagcgccag caaaccgtgc agcgcttcgc cggattgtgc cggggataac tgcgccagca
     7321 tcgtcagcgc cgcttcggca ctagcgttgg gatgttccag acgcatcagt gcgccaagag
     7381 ccgcgccgcg atccagcgcc ggggcatgga ttgcctgact gcgtcgctcc agtaaggcta
     7441 ccgccgcaga aagagaaacg ccgggtaaat taacgccgct atgtagatcg cgcagaatat
     7501 ggcaaagcgc ttgccagcta tgcaggtgag cgtgaaactc cttttcatcc ggtctgccgt
     7561 tagattcaca cagccacagc gtgcgatcga tagccgcgca taacgtcgtt tgtgatatct
     7621 gcgcaccttg cataccgcta atttcatcca gccgccataa ggcatatagc acttccagcg
     7681 ccgggcccat ttcggcaaat tgattttcct aggcgattaa taatgtgagt tgctccagta
     7741 attgttgact gaaggacgcc agacccgcta acgccgcctg gctaagacat gtggtgatac
     7801 tgccgattcc acccgcgtcc agcatatcgg cttctaattt atggcgtgcg gcttcctgga
     7861 gtgtggcacc aaagcaggcg gcctcaatca atgccgcatg ctggctaagc gggcgggtta
     7921 atttccagcg ttcttcaccg ttgcctgcaa gtgtcagtgt gcttccctgc tggcgtacaa
     7981 tccctgggat ctccagtatt gccagccgat gtaacacctg actttgcgct agtccattgg
     8041 ggttaaagcg atttaaggta agctccgccg aaagagagat tgcggtacgt tccagttcgc
     8101 aggtgacatc ttttggcaga ggcggttgtg gcgtagaagg ggcaagttta ccgaatccgt
     8161 cacccgctaa tgtgtctatc aacgttagca gaatcggatc ggtatcggga tgaataacgc
     8221 cgcgatagct ccacggcaac ggcgcgttca gggcttcttt aatcagcgag cctgctatgg
     8281 catccagcca gtcagtgcgt aacggtagtt tatatccgcg caactgtgcc agcgccatcg
     8341 catgcagatg agcggcagcc atatccgcgg tcgaagcagg caagtggtgt tggcgcaaac
     8401 gggtaagaac cgtttttagc agttgttctc ccgcctgctg taagccccac tgccagcacc
     8461 agctttgcca gaccggggca ggcattcctg aaaggtatcc tgccagcaca tcaaggcgct
     8521 tttcactgta gggtgtgaga taacaacctg taacggcatc tgccagcgag ggcaattctg
     8581 gcttgttaat gtcctgcggg cattcgcgcc acatttttgc cagtgccgga gcgtgccagc
     8641 caccgcagac gactaacacg tcgccattat tttgctgcac tgcccatgca atccagcgag
     8701 ccataaaggc ttcgcgctga cgattgagtg catcgccggg gaaatcgccc cgcagttggg
     8761 caaaatagtg cgccagtgca gagggtaatg cagtttgctg gctttcatct tcgaacaagt
     8821 gatcccacag ggtatcgctg ttatccatgc gggtggcacg cagcagcaag gcctggcttt
     8881 cctcttgcgt atcaggcgaa tcgtcctctt cttcactctg cgcccagcaa ggcaaatcga
     8941 taaagtaagt ttgtgcctga atatgacgtg cggcttgtag cgcctgccac tccggcgaaa
     9001 attcggcaaa tggcgtccag gcgccgcgcc caggagcagc accgtcctga tactggcaat
     9061 aactgtaaat agccaccgga agctggtggg ctaaaaacag ttcatctacc cggtcattaa
     9121 aatcagccgg gccttcaatc aacacgtatc gtggtcgctg gctttcgatt aacgatttca
     9181 ccagtcgggc gcaggccgga ctatgatgcc ggatgccgac aattaacggc tcgctcatgc
     9241 atctttcctc acggcaggcg gtggcgggct tgataataag cctgccagtg agcttcttta
     9301 tgtgtggcga cacgctgttc gaagtaacgg cgcagacgag cgcgatcttc ttcgttatct
     9361 ttgacgatgg ttccggcaat acagtccacc aggtcggcag gctcccccgc gcgattcgct
     9421 aaaaaccagg cgcggacgcc cacagcgtgg gccacgttca cggcttcagc ggtggacata
     9481 atcgctgtta gtgtatccat tgacgttttc ttttcgccgt tggcacgcag atcgcggaag
     9541 gtgcggacca gtaactccag taccgcgtca ggcactttat gcggaatacc gctatgggcc
     9601 agcaaacgcg ccgaagcact ggcgacaagc tccagctcct gggcgaaatc cataatcgga
     9661 aagacagttt caaaatcgaa gcgacgcttt agcgcagcac tcatttcatt gacgccgcga
     9721 tcgcgggtat tggcagtggc aataatatta aagccttctc gcgcatagag ctgactggct
     9781 tcaccagtga gttccggcac cgtcatcacc ctgtcagaaa gcatacctaa cagacagtct
     9841 tgcacttcca gcggtgtacg cgttatctct tcaaaacgga cgattttgcc gtcgcgcatt
     9901 ccctggtaaa gcggggcagg gaccagcgct tccgttgatg gaccatgatt gataagcagg
     9961 gcgtagttcc agccgtactt gatttgatct tcggtagtag atgcgccgcc ctggatggtt
    10021 aacccggcat cgccgctaat agaggttgcc agtaattcag acaggagaga ttttgcggtg
    10081 ccaggttccc ccaccaacat caaaccgcgt ccggtggcaa gagtgactaa catacgttcg
    10141 acagcggagg gactaatcac cactttacgg ctaatatttt gcgcgctatc gccaaggata
    10201 aaagcacggg ccgcaggcaa acttaactgc caaccgggcg ggcaaggtgc gttatcattt
    10261 tgttttaatt ttgccagttc atcggcgtat aacacagcag caggcggacg ctgcagatga
    10321 ttattctgcg gtgacatata ttcatccctg aaatgtatta atgaaataaa cgatatttta
    10381 cgcgcaataa atgtttggcg cattagttgt ttgagcgaaa aatatctggg aagcagatca
    10441 cagtaaataa aaaaacctgc tggtgaggca ggttctttta tttactattt tcaggcctaa
    10501 caccacagcc tgtgcaacat cgtgttccaa cgaacgaatt ataaccacgg ctggaccccg
    10561 ttgcaattaa gataatgaga tttataatcc tgactgttgg gacgttgcgc cggatcgaac
    10621 ctatttcaag tcctggctac cggacggggg gtgctgcagg tggtgccctg atccggagtg
    10681 caacatcgtg gtccaacata cgtaaaccag tatcgacagg cagtgtaagg gtaaggtgct
    10741 ggtgttgcgc tccttaataa ggagagcgga tggactcgct gacacaaaag ttaaccgtgc
    10801 tcattgctgt act
//
LOCUS       DAZFBR010000139        10591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFBR010000139 DAZFBR010000000
VERSION     DAZFBR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.139"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..965
                     /locus_tag="ACHJZ2_004214"
     CDS             <1..965
                     /locus_tag="ACHJZ2_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4570217.1"
                     /translation="LKDLERAYKNFFRKRAAFPRFKKRGQNDAFRYPQGVKLDQENSR
                     IFLPKLGWMRYRNSRQVTGVVKNVTASQSCGKWYISIQTENEVSTPVHPSALMVGLDA
                     GVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCI
                     ANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSIL
                     DQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQACGY
                     TANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            1009..1116
                     /locus_tag="ACHJZ2_004215"
                     /pseudo
     CDS             1009..1116
                     /locus_tag="ACHJZ2_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1100..1738)
                     /gene="ytfB"
                     /locus_tag="ACHJZ2_004216"
     CDS             complement(1100..1738)
                     /gene="ytfB"
                     /locus_tag="ACHJZ2_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO4570218.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1956..2576
                     /gene="fklB"
                     /locus_tag="ACHJZ2_004217"
     CDS             1956..2576
                     /gene="fklB"
                     /locus_tag="ACHJZ2_004217"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4570219.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2885..4297
                     /gene="cycA"
                     /locus_tag="ACHJZ2_004218"
                     /gene_synonym="dagA"
     CDS             2885..4297
                     /gene="cycA"
                     /locus_tag="ACHJZ2_004218"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO4570220.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(4342..5004)
                     /gene="ytfE"
                     /locus_tag="ACHJZ2_004219"
     CDS             complement(4342..5004)
                     /gene="ytfE"
                     /locus_tag="ACHJZ2_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO4570221.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(5112..6077)
                     /gene="ytfF"
                     /locus_tag="ACHJZ2_004220"
     CDS             complement(5112..6077)
                     /gene="ytfF"
                     /locus_tag="ACHJZ2_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4570222.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(6185..7045)
                     /gene="qorB"
                     /locus_tag="ACHJZ2_004221"
     CDS             complement(6185..7045)
                     /gene="qorB"
                     /locus_tag="ACHJZ2_004221"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO4570223.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            7134..7514
                     /gene="ytfH"
                     /locus_tag="ACHJZ2_004222"
     CDS             7134..7514
                     /gene="ytfH"
                     /locus_tag="ACHJZ2_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO4570224.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGGLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(7632..9575)
                     /gene="cpdB"
                     /locus_tag="ACHJZ2_004223"
     CDS             complement(7632..9575)
                     /gene="cpdB"
                     /locus_tag="ACHJZ2_004223"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO4570225.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            9765..10505
                     /gene="cysQ"
                     /locus_tag="ACHJZ2_004224"
     CDS             9765..10505
                     /gene="cysQ"
                     /locus_tag="ACHJZ2_004224"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO4570226.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2453 a         2689 c         2797 g         2652 t
ORIGIN      
        1 cactgaaaga ccttgagcgg gcttacaaaa acttcttccg gaagcgggct gcttttcccc
       61 gattcaaaaa gcggggacag aatgatgcat tccgctaccc gcagggcgtt aagctcgatc
      121 aggaaaacag ccgtattttt ctgccgaaac ttggctggat gcgctaccgg aatagccgtc
      181 aggtcacggg tgttgtgaaa aatgtcactg ccagccagtc ctgcggtaag tggtacatca
      241 gtattcagac tgaaaatgaa gtatcaactc cggttcaccc ttcagcatta atggtcggac
      301 tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg cacagtcttt ggacctgtaa
      361 acagttttca gaaaaaccag aagacgctgg cgagacttca gcgacagtta agccgcaagg
      421 tcaaattcag caacaactgg cagaagcaga aacgcaaaat acagcgactg cattcctgta
      481 tcgcaaatat ccgcagggac taccttcaca aagtcacaac gaccgtcagc aaaaaccacg
      541 caatgatagt cattgaggat ttgaaggtca gcaacatgtc aaagtcagca gcgggtacgg
      601 tcagccagcc ggggcgcaat gtccgggcaa aatcaggttt aaaccgttcg atactggatc
      661 agggctggta tgaaatgcgc cgccagcttg agtacaagca gctctggcgt ggcggtcagg
      721 tgcttgctgt tccgccagcg tacacaagcc agcgttgcgc gtgctgtggt catacagcga
      781 aagaaaatcg cctgtcacaa agtaaattca gatgccaggc atgtggatat acagcgaacg
      841 ctgatgtaaa tggcgctcgt aacattttag cggcggggca cgccgttctt gcctgtgggg
      901 agatggtgca gtcaggccgc ccgttgaagc aggaacccac cgaaatgatt caggcgacag
      961 cctgaacgta gcagggatcc acgtccttca gggcttggag gatgtcaaca catcagtatg
     1021 gtttcacccc aggcactgga agccagacgt aaggtcatca aagagacaat ccttagctgg
     1081 cttttggttg acccttcatc taccgcgcac gaataaaact gccatccggc tggcgggtga
     1141 acaggacctg ttgattattc cccgtatcaa tggttaagcc cgtcaccacg ccgctggcgt
     1201 tctggcggat tttcaccatc tgcccgtttt gcaaattact cagcggctta cccgcgcctt
     1261 ctacctgcgc cattgcatag acatcggtag cgggcagtcc gtggtcgcgg aacagctgcg
     1321 ccatcgtttt accgggttcg acacggtaag agcgccattg attatcaatt ccgctatctg
     1381 gctggaatgg ttgcgtttgg gtagttttcg gctgttcttc tggctggcct tcctgtatag
     1441 gttctggagc gaccggagcc acctgatcag gataattttg tggggtgact aattgcgccc
     1501 gcagctgttc ttctgtcggc ggttgtgatt gcgactgaat atctaactgc gcttcacgcg
     1561 tcaccaccgg cgcgttgggc gtatcatcag atggaagcag aaatccgacc accaatacaa
     1621 tggcggcaat aatgatcccc cgacgatgca tcggcggcag cgggtccatg aagcgaaaat
     1681 tgtccggtgc gtgccagact ttctccaggg ttggttttag ttcaaagcgc ccgggcatgg
     1741 tttcctcctg ctccgcgtct tgttcctcaa tcatagccta tgaataagct aacgctatga
     1801 tgtccgtggt aaacccgcct ttattatatt catacgggat attgctattg tttctttttc
     1861 cctgggattt gtcatcattc ccgcgacaaa gttttaccca aagaagtgtg gctgatatgc
     1921 tgcccgctac tttataccct aagaaaggaa atacgatgac caccccaact tttgacacca
     1981 tcgaagcgca agcaagctac ggcattggtt tgcaggtagg gcaacaactg agtgaatctg
     2041 gcctggaagg gctgctgcca gaagcactgg ttgcaggtat tgccgatgcg ctggaaggca
     2101 aacatccggc tgttccggtt gatgtggtgc atcgcgcgct gcgtgaaatc cacgagcgcg
     2161 ccgatgccgt tcgtcgtcag cgtttccagg cgatggctgc tgaaggtgtg aaatacctgg
     2221 aagaaaacgc caaaaaagaa ggtgtgaata gcaccgaatc tggcctgcaa ttccgcgtga
     2281 tcaaccaggg tgaaggcgca attccggcac gtaccgaccg cgttcgtgtt cattacaccg
     2341 gtaaactgat cgacggcacc gtgtttgaca gctccgttgc tcgtggtgaa cccgctgaat
     2401 ttccggttaa tggcgtgatc cctggctgga ttgaagcact gactctgatg ccggtaggtt
     2461 ctaaatggga actgactatc ccgcaggaac tggcatatgg cgagcgcggc gcaggcgcat
     2521 ccatccctcc gttcagcacc ctggtgtttg aagtcgaact gctggaaatc ctctaagcag
     2581 cgcattctgt tcccctcgaa ccagagggga gcaggcattc agcaataaac ccttcagttt
     2641 gccaaacggc gctattttgt gttgcaaaga ccccgtaagc gtgtattttt gtgagctgtt
     2701 tcgcgttatc accgtgatat gacactcact ttaaacataa aattaacatt agatctaaat
     2761 cttagtattc atcccgcgta ttgttaccta atatcgatga gtcccgatac agattcgtcg
     2821 tatcatagac tgactaaagg ccgtagagcc tgaacaacac agacaggtac aggaagaaaa
     2881 aaacatggta gatcaggtaa aagtcgttgc cgatgatcag gctccggctg aacagtcgct
     2941 acggcgcaat ctcacaaacc gacatattca gcttattgcc attggtggtg ccattggtac
     3001 ggggttgttt atggggtctg gcaaaacgat tagccttgcc gggccgtcga tcattttcgt
     3061 ttatatgatc attggtttta tgctcttttt cgtgatgcgg gcaatggggg aattgctgct
     3121 ttcgaatctg gaatacaaat cttttagtga cttcgcttcc gatttactcg ggccgtgggc
     3181 aggatatttc accggctgga cttactggtt ctgctgggtt gtaaccggta tggcagacgt
     3241 ggtggcgatc acggcttatg ctcagttctg gttccccgat ctctccgact gggtcgcctc
     3301 gctggcggtg atagtgctgc tgctgacgct taacctcgcc actgtgaaaa tgttcggtga
     3361 gatggagttc tggtttgcga tgatcaaaat cgtcgccatt gtgtcgctga ttgtcgtcgg
     3421 cctggtcatg gtggcgatgc actttcagtc accgactggt gtggaagcgt cattcgcgca
     3481 tttgtggaat gacggcggct ggttcccgaa aggtttaagt ggcttctttg ccggattcca
     3541 gatagcggtt ttcgctttcg tggggattga gctggtaggt acaacagctg cggaaaccaa
     3601 agatccagag aaatcactgc cacgcgcgat taactccatt ccgatccgta tcattatgtt
     3661 ctacgtcttc gcgctgattg ttattatgtc cgtgacgccg tggagttcgg tagtcccgga
     3721 gaaaagcccg tttgttgaac tgtttgtgtt ggtaggtttg cctgcggctg ccagcgtgat
     3781 caactttgtg gtgctgacct ctgcggcgtc ttccgctaac agtggcgtct tctctaccag
     3841 ccgtatgctg tttggtctgg cgcaggaagg tgtggcaccg aaagcgttcg ctaaactttc
     3901 taagcgcgca gtacccgcga aagggttgac tttctcgtgt atctgtctgc tcggcggcgt
     3961 ggtgatgttg tatgtgaatc ctagcgtgat tggcgcgttc acgatgatta caaccgtttc
     4021 cgcgattctg tttatgttcg tctggacgat tatcctttgc tcgtaccttg tgtatcgcaa
     4081 acagcgtcct catctacatg agaagtcgat ctacaagatg ccgctcggca agctgatgtg
     4141 ctgggtatgt atggcgttct ttgtgttcgt ggtcgtgttg ctgacactgg aagatgacac
     4201 tcgccaggcg ctgctggtta ccccgctgtg gtttatcgcg ctggggctgg gctggttgtt
     4261 tattggtaag aagcgggctg ctgaactgcg gaaataaccg cattatcatg ctggatggcg
     4321 caatgccatc cagcttttag atcactcacc cgccagcgcc cttgggaaca gtacattgtt
     4381 ttccaggctg atgtgatcca tcaggtcatc aatcagttca ttaatgccgt tatacatcgc
     4441 tttccaggtg gtgcaggctt ccggcggcgg tgtgacgtta ttggtattgt gtttgatcac
     4501 ttccagcagt tcacccgctt catcgtgctc gctttccatt acgctgattg gccccattgc
     4561 ctggctgccc atgccttgtt tgatcatcgg gaagaggatc tgctcttctt tcatcatgtg
     4621 gctggaaagc tcttcatgca gcatggtcag gtattttgtc agcccttttg gcacgctcgg
     4681 tttgtcggcg tgaacgcgct cgactttagt cgcttgcaga atcagctccg gcagttgctc
     4741 gcggtgacga gcgtggtagc gcacgatgat atggtcgatg atttctgcca gcggggcgct
     4801 acgccagtct ttctcaatcg gttgttcagc aagctttgcc agctcagctt caatgacctc
     4861 aacatccagt tctttacgtg ccgccgcacg cgccagcgtt tgcttaccgc cgcagcagta
     4921 atccatatca tatttacgaa acagagccga agcgcgagga atagagagcg ccagttcacc
     4981 taaaggttgg tcgcgataag ccatagctga tacctcattc ttaataagat aagatgtatt
     5041 ttaaatgcat ctttaaggca aaagctataa cccttacgta gcagaggctt atttaactca
     5101 ctgccagcgt gtcaactttc tgagacagat tcagttaaag gtttttctgg ttttgctcta
     5161 accgcgatga ccacgccaat cactaacagc gcgataccgc tcagcgtcat tagcggcggc
     5221 atttgctggc ggagtaaaaa ggtgtacagc aaacctgcca gcgtttcgaa aacaatcagc
     5281 ggcccgagaa tcactgtcgg taatcgctgg ctggcgacgt tccagcagag tgcgccaacc
     5341 catgagcaaa gcacggctat cgcaatcatc agactaataa acaccagcgg acgggggcca
     5401 aagggtaggg agaagtccgg cgtttgcgta ttcagccagt aacaagcgac gagatagccg
     5461 ataagtgaaa ccggcagtgt gaccagcgcc tgcgctgttg cccacatcat cggatgtttg
     5521 tcgggatttt cccgcagcca gcgggcgttg cgcagggcat accacgccca gcagaccacg
     5581 gaaactaacg ccagcacgat gccagaggta taacgtgccc agtcaaaatt ggggagtccg
     5641 tggtttaact cagcaatatt cacacacgcc aggccgatgc caatacaaat cagtgccggg
     5701 gcgagttttc cccacgcgag tttgccgtcg cgctggctat aaagcagatt ggcgaagacg
     5761 ggaatcacca ccggcagggt gccgataatc atcgtggaaa caggcgcacc agtacgttga
     5821 atggcactgg caaggcagaa gtaatagatg aggttgccca tcatagtgag catcaaggcg
     5881 gtaagccagt cgcgacgcgc caactgacgc agacgcacgc gtcccagcca ggcaatgggc
     5941 agcgcaatta accctaacgc cagataacgc cccatcgact gcaacatcgc cgggtattcc
     6001 ggcacgatca acggcccgac aaaaataagc ccccacatca accctgctaa cagggcgtac
     6061 agcacgccgc taatcattac tggcatccat tgatctgtca gaagaaagtt caggaagcag
     6121 acatgattgc tcccggcatc ccgtcattat cagagaggat gcgggaggat gccactttaa
     6181 ttaactagtt attaacatta aaaagatggc ttacgctttc ggctaacgtt gtcgttgggt
     6241 ggccaatcaa tttgctaagt gttttgctgt catcaaacag accgcctttc gatgcgccaa
     6301 cgtcagaatc cgccagcata tccgccagtc cgtcgggcag tccgacgctt ttcagtgccg
     6361 cggcgaaatc ggcttcgctc agattttgat aggtaacctg tttgccgctc tgtttggtca
     6421 gctctgccgc taactgtgtc aacgtccagg cactatcgcc cgccagttcg taaaccttgc
     6481 cttcgtgacc ggcttcgcta atcacgcgtg ccgcagctgc cgcataatct gcccgcgttg
     6541 ctgaggcgat tttgccatcg cccgccgcac cgataaatac gccgtgttcc agtgctgccg
     6601 gggcgctggc gaggtagttt tcggtgtacc agccgttgcg cagtagggtg taaacgatgc
     6661 cagaatcagc cagcattttc tccgtctcga tgtgctcatc ggcgaggccg agcggagatt
     6721 tatccgcgtg aagcaggctg gtataagcga taaatttcac gccagccgtc tttgcggcat
     6781 taataacatt acgatgctgc ggggcacgtt gacccacttc gctggaagag atcagcagta
     6841 gtttttccac tccctgaagt gcagatgtca gtgcggcttc atcgccgtag tcagcctgac
     6901 gcacggtaat gccttgtgct gtcagtgcct gggcttttgc cggattacga acgatagcca
     6961 ctatttggct ggcaggaacc gttttcatca aggattcaat aacatactga ccaagttggc
     7021 cagtggcacc agtaatagcg atcatgggaa gtctccatcg ttttgtcttg tgtttaaata
     7081 cgctaacacc taaacttact tttagtaagt acgcacaaaa aggtaagtat gaaatgagtc
     7141 aggttagcct gtcgcaacaa ctgaaagagg gtaatctctt tgcggaacag tgcccgtcgc
     7201 gcgaggtgtt gaaacacgtc accagccgtt ggggggggtt gattctggtg gcgctacgcg
     7261 aaggtactca tcgctttagc gacctgcggc gcaaaatcgg cggggtgagc gaaaagatgc
     7321 ttgcgcagtc gttacaggcg ttagaacagg atggttttct taaccgtatc gcgtatccgg
     7381 tggtgccgcc gcatgttgaa tacagcctca cgccgctggg cgagcaggtg agcgaaaagg
     7441 ttgctgcact ggcggactgg attgagttga atttgcccga ggtgttggcg gtgcgggatg
     7501 aacgtgcggc ataactgccg gatgcgcggc ctacggtgta tgacttgtag ggcggataag
     7561 atgcgccagc atcgcatccg ccaataaatg ccggatgcga ttgatgattt ataggccgaa
     7621 aaagaagtgt tttacttact caaatccacc tgataaatcg caaacccgat atcatcggtc
     7681 gcgactttat tcatcggata ctgccctttc tctttaataa acgctgcggc tttatctgac
     7741 ggggaagttt cgaaacggat atccagtttc ttatcggcag ctatcggtgc taaacgccag
     7801 ttgttatctg ccgccgggtg aatttccccc gcacgtttcg actcatcagc aatccacgct
     7861 gccagcaccg agcggttctc atccggtgaa gcaaaagcga tatggctgtc gcccgtcccg
     7921 gcaaatttgc cgccgtaagc gcgatagtta ttggtggcaa cgaggaacat agcgttcgga
     7981 tcaatcggct tgccattaaa ggtcaggttc ttaatccttt ccgcattggc attaatcatc
     8041 tggcactcgc cgtcataacg ggcgggctgg gtaacatcaa tctgataatt cacaccatca
     8101 ataacatcaa agttataagt gcggaaacca tcccagttga tgagtgactg tggtttcgtg
     8161 ctgttgggat caatctggtt aaactgtccg gcggagcact ccagccactc tttcacctct
     8221 ttaccgctgg ctttcaccac aatcagcgta ttgggataga gataaagatc ggcggcatta
     8281 cggaaggtca actggccttt ttccacctcc acatagcttg ccgggtcatt tttgcgacca
     8341 ccgactttaa acggtgcggc agctgaaagc accggcagtt ttgccagatc cggatcgccc
     8401 tgaatgtaat gctcgacata cgctttttgc gcgttgttca ctacttgcac ggtcggatcg
     8461 tcctgcacca gcgccagata gctatacata ttgtcggcag atttaccgat tggcttgctg
     8521 acgaactggc gtgtggcatc gtgatcggct ttgagtgttt ctaccagctt gctgtcttcc
     8581 gccgcgaggg attttttatt ggcgatgtcg taaatcggcc gtgcttccgc tttcgcctgc
     8641 gtcacctgcc atttaccgct gtcattactg agttgtaagt cgaccacccc aagatgatcg
     8701 ccccacatgc ccggcattac cgccggaaca ccattcagcg tgcctttggt gatatcagcc
     8761 ccttcgatat cagcaaaatc tttacccggg aaaacggcgt gagcatggcc aaacataatg
     8821 gcgttaacgc ccggaatttc actgaggtaa taaactgagt tttccgccat tactttatac
     8881 ggatcggcag acagcccgga atgcgccaga acgacaacga catcggcacc tttctcgcgc
     8941 atttcaggct cgtatttgcg cacggtttcg gtaatatcat tcaccgtcac ttttccggat
     9001 aaattagctt tatcccagcc catgatttgc ggcggcacga cgccaatata gccaatcttc
     9061 agcgtctgtt tttttccgtc tttatcgacc acttcggtgt ctttaattaa atacggtgta
     9121 aacattggct gtttggttct ggcgtcaatg acgttggcat ttacataagg gaatttcgct
     9181 cctgccagcg catttttcag gtaatccaga ccgtagttaa actcgtggtt gccaagcgtt
     9241 ccgacggtat agtccagcgt atttaatgcc ttatagaccg ggtgaatatc acctgctttt
     9301 aatcctttcg ccgacatgta atcggccagc ggactcccct gaatcaaatc gccgttatcg
     9361 accagtacgc tgtttttcac ttcattgcgg gcattgttaa tcaggcttgc cgtacgtacc
     9421 agtccgaatt tttccgtggc ggtgtctttg taataatcga aatccatcat gttgctatgc
     9481 agatcagtgg tttccatgat acgcagatcg accgtcgctg cattcacact ggcggcaatc
     9541 agcgtggcca ggagcgttgc gctaaactta atcatcaggg acatcctttt atcatcggga
     9601 atacgaaaga aaagggagaa taaacgtctt acttatagaa cagtgaagaa tgccacaatt
     9661 ttacgctttg aaaatgatga cactatcaca gttggcgcat tcattaatga tagggtataa
     9721 gtaaaacaat aagttaacac cgctcacaga gacgaggtgg agaaatgtta gatcaagtat
     9781 gccagcttgc acggaatgca ggcgatgcca ttatgcaggt ctacgacggg gcgaaaccga
     9841 tggacgtcgt cagcaaagcg gacaattctc cggtaacggc agcggatatt gccgctcaca
     9901 ccgttatcat ggacggttta cgtacgctga caccggatat tccggtcctt tctgaagaag
     9961 atcctcccgg ttgggaagtc cgtcagcact ggcagcgtta ctggctggta gacccgctgg
    10021 atggtacgaa agagtttatt aaacgtaatg gcgaattcac cgttaacatt gcgctcattg
    10081 accatggcaa accgatttta ggtgtggtgt atgcgccggt aatgaacgta atgtacagcg
    10141 cggcagaagg caaggcctgg aaagaagagt gcggcgtgcg caagcagatt caggtccgcg
    10201 atgcgcgccc gccgctggtg gtgatcagcc gctctcatgc ggatgcggag ctgaaagagt
    10261 atctgcaaca gcttggcgaa catcagacca cgtccatcgg ctcttcgctg aagttctgcc
    10321 tggtggcgga aggacaggcg cagctgtacc cgcgtttcgg accaacgaat atttgggaca
    10381 ccgccgctgg acatgctgtg gctgcagctg ccggagcgca cgttcacgac tggcagggta
    10441 aaccgctgga ttacactccg cgcgagtcgt tcctgaatcc ggggttcaga gtgtctattt
    10501 actaaattca gatggcagaa acagtgtatt tcctgattct gccatcctga tttctcccaa
    10561 cctaaaaagt tataaataaa aagagattgt a
//
LOCUS       DAZFBR010000140        10565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFBR010000140 DAZFBR010000000
VERSION     DAZFBR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.140"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..198
                     /locus_tag="ACHJZ2_004225"
     CDS             <1..198
                     /locus_tag="ACHJZ2_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4570227.1"
                     /translation="FNKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRESV
                     FWLNVKNIPPSIDKKIPMFLR"
     gene            351..500
                     /locus_tag="ACHJZ2_004226"
     CDS             351..500
                     /locus_tag="ACHJZ2_004226"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4570228.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            522..3056
                     /locus_tag="ACHJZ2_004227"
     CDS             522..3056
                     /locus_tag="ACHJZ2_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4570229.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            3068..4138
                     /locus_tag="ACHJZ2_004228"
     CDS             3068..4138
                     /locus_tag="ACHJZ2_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4570230.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            4166..5248
                     /locus_tag="ACHJZ2_004229"
     CDS             4166..5248
                     /locus_tag="ACHJZ2_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4570231.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            5460..6500
                     /gene="pstS"
                     /locus_tag="ACHJZ2_004230"
     CDS             5460..6500
                     /gene="pstS"
                     /locus_tag="ACHJZ2_004230"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO4570232.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6587..7546
                     /gene="pstC"
                     /locus_tag="ACHJZ2_004231"
     CDS             6587..7546
                     /gene="pstC"
                     /locus_tag="ACHJZ2_004231"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO4570233.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7546..8436
                     /gene="pstA"
                     /locus_tag="ACHJZ2_004232"
     CDS             7546..8436
                     /gene="pstA"
                     /locus_tag="ACHJZ2_004232"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO4570234.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8619..9392
                     /gene="pstB"
                     /locus_tag="ACHJZ2_004233"
     CDS             8619..9392
                     /gene="pstB"
                     /locus_tag="ACHJZ2_004233"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO4570235.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9407..10132
                     /gene="phoU"
                     /locus_tag="ACHJZ2_004234"
     CDS             9407..10132
                     /gene="phoU"
                     /locus_tag="ACHJZ2_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO4570236.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            10370..>10565
                     /locus_tag="ACHJZ2_004235"
     CDS             10370..>10565
                     /locus_tag="ACHJZ2_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570237.1"
                     /translation="MEKKTKNRVKALYRDIKNERFFTVFIGGLVIIVANQHPGMLFKT
                     NRQCIMLGRINIVFRRNDYGY"
BASE COUNT         2724 a         2502 c         2662 g         2677 t
ORIGIN      
        1 tttaataaag aggataaaag caaaccgcca ttttctgtga tacctcccgt ttctcgcatt
       61 gaaccacagc aagagaaaat attacgcatt ttaaaaacgg ctggcgagtt accaaaagat
      121 cgggagtccg tattctggtt gaacgtgaaa aacatccctc cttcaattga taaaaaaata
      181 ccaatgttct tgagatagcc ataaaaacaa gaattaagtt attctggcga ccagccgcgg
      241 taaagataat ccctgagcag gctattaaaa atgttaaatg gtcgcggcag ggtaataatc
      301 tcatcgtcga aaatccaaat ccgatccata ttaatgtaat gagtgtgacg gtggatgata
      361 aagacgtgtc gctaaatatg atccgccctt ttgaaatatt gacattacca attccggctg
      421 gcgtggcagg aaaatcgctc gtttggcgct ttattaatga ttacggtgca attagtcaac
      481 cactaaagaa aaacctttaa gcgccggata aggatatatc gatggcctct tcgcatcttt
      541 tcattacgct tgcatcgggc atatgtctgc tctgttccat atctgctttt gcccgggata
      601 gcttgttcaa ccccagatta ctggaactgg atcatcctgc ggataatatt gatattcacc
      661 agttcaaccg ttcgaatacc ttacctgcgg gaacatacaa agttgatgtg atgatcaacg
      721 gcatgctctt cgaacgccag gaagttaaat tcgcccagga taaccctgat gctgaactcc
      781 acccatgcta cgtggcgata aaaaacgtgc tggcgaccta tggtataaaa gttgatgcga
      841 taaaatctct ggcgaatgtt gatgacaaaa catgcgtaaa tccagttccg ctgatcgacg
      901 gggctacctg gttactggac gccagtaaac ttgcattgaa tattactatt ccgcaaattt
      961 atctcaacaa tgcagttaat ggttatatca gcccttcccg ttgggatcag gggatcaatg
     1021 ccatgatgat gaattatgat ttttcggcat cgcataccat ccggtcaaat tatgacgacg
     1081 acgatgacag ttattatctg aatttgcgta atggtattaa tttaggcgca tggcgttttc
     1141 gtaattacag caccctgaat tcttatgacg gtaatgtgga ctaccattcc gtcagtaatt
     1201 acattcagcg cgacatcatg gcattacgta gccagattat gattggcgat acctggacgg
     1261 caagcgatgt atttgatagt acacaggtgc gtggcgtgcg gctgtatacc gatgacgata
     1321 tgttgccctc cagccagaac ggctttgcgc cagtggtaca tgggattgcg aaaactaacg
     1381 ccacggtgat catcaaacaa aacggctacg ttatttatca atcagccgta ccacagggcg
     1441 catttgccct caccgactta aacacgacca gtagcggcgg cgatctcgat gtcactatca
     1501 aagaagaaga tggcagcgag cagcacttta ttcagccatt tacttcactg gccattctca
     1561 agcgtgaagg tcagaccgat gtagacctta gcattggaga agtgcgcgac gaaagcggct
     1621 ttacgcctga ggtcttgcag ttacaagcaa tgcacggttt ccctttggga ataactttgt
     1681 atggcggaac acaattggca aatgattacg cttctgccgc gctgggtatt ggtaaagata
     1741 tgggggcgct gggcgcgatt tcttttgacg tgactcatgc ccgctcgcag tttgactacg
     1801 acgataatga gagtggtcaa tcgtatcgtt ttctctattc caaacgtttt gaagacacca
     1861 ataccacctt tcgtctggtg ggttatcgct actctatgga ggggttctac accctcaatg
     1921 aatgggtgtc gcgacaggat aatgattctg atttctgggt aacgggcaac cgtcgcagcc
     1981 gcttcgaagg cacctggacg caatctttca cgccaggctg gggcaatatt tatttaacat
     2041 tcagtcgaca ggaatactgg cagaccgatg aggtcgaacg tttattacag ttcggctata
     2101 acaacaactg gcgaaacatc tcctggaacg tttcctggaa ctatacggac tcgatcaagc
     2161 gctcattggg caaccatcat gatgataaca atgatgattt cggcaaagaa cagattttca
     2221 tgttctcaat gtcgataccg ctatcgtgct ggatggaaga cagctacgtc aactattcgt
     2281 taacgcaaaa caaccaccat gaaagcacga tgcaggtcgg tctgaacgga acgatgctgg
     2341 aagggcgtaa cctgtcttat aacgtacagg aatcgtggat gcactctcct gatgactcct
     2401 acagcggcaa tgccggaatg acctatgacg ggacttatgg ctcggtcaat ggtagctatt
     2461 cctggagccg tgactcccaa cattttgatt atggcgccag aggcggcgtg ctggtgcata
     2521 gtgacggagt gaccttctcg caggaactgg gcgaaacggt ggcattggtc aaagcgccgg
     2581 gcgcagaagg cctgtccatt gaaaacgcca ccgggatttc taccgactgg cgtggttata
     2641 ccgtaaaaac gcagcttagc ccgtatgacg aaaaccgcgt ggcattgaac agcgactatt
     2701 tctccaaagc caatattgaa ctggaaaaca ccgtcatcaa cctggtacca acgcgcggtg
     2761 cggtggtgaa agccgaattt gtcacccatg tcggttatcg cgtgctattt aacgtccgcc
     2821 aggtcaacgg taaaccaata atgtttggcg cgatggcaac cgcctctctc gaaacgggca
     2881 cagtcaccgg gattgtcggt gataacggcg aactgtatct ctccgggatg cctgaaaaag
     2941 gcgagttttt attgagttgg ggacaagctg cggatgaaaa atgtaaggcg gcctatcaca
     3001 tcacccataa acctgatgat accagcctgg ttcaaatgga tgcgatttgt cgctaaagga
     3061 agcatgaatg aaaaaaatat tatcagggtt gattctgctg ctttgctgtc cttatggttt
     3121 cgccgctaat ggtgatggcg caacgcacat gtcaaattta tcatttggtc cgctgacggt
     3181 ggcagcggcg aataaccact ccggatacaa tattttcgag gcactgagca acacgactgg
     3241 aacatacccg gtgcgctgtc actgtgatga cacgcatggc ggaccgggcc aacaaacagc
     3301 attttttcct atcttctaca cgggagatgc cgcaccgggg cttgtgcttg agcgcactct
     3361 taatgggtta aattactatg ctctgaatga ttatttatcg gtcggcgtga cgatttttat
     3421 tattaataac caatatgcgg ccattccttt tgaacactta tccaaccaat ccacctcacc
     3481 gcaacatacc tgtggagcag gtaataatgg gagcactgta aatctggatt cagggcgctc
     3541 ggcaaaatta tctttctatg ttcggcattc tattactggc acggtgacaa tacccacaac
     3601 ggaagtcgcc tggttgtacg cgggcatgtc cgatcatttt cccaaaacga cccccgtttc
     3661 taaagtgaca attcgcggac aactaacggc cccgcagaac tgtgagttaa cgccaaatca
     3721 gagcatcgat gtcgattttc aaaaaattaa tagcgctgag ttctcctcaa cggcgggttc
     3781 aattattgca gaaagaaaga ttaaaaccga agtcacagta tcctgtaccg ggatggaaga
     3841 cgtaaggtcc acggaggtgg tgagtgcgtc gatgattgcg gcaaacagaa gtgccgatgc
     3901 caccatgatc gtgacgagta atccggatgt gggaattaag atttttgata agaacgaccg
     3961 tccagtgaat gtggatgggg gcaacttacc cgctgatatg ggtgctatta gtcgattagg
     4021 aaaaaccgat ggtagcgtaa cgttttattc agcgcccgcc agtctgacgg gcgcaaaacc
     4081 agcgcctgat aatggattta ccgctacagc cacgctggtt attgaattta ctaactaatc
     4141 ttgtgttagt aaaaggaata atttcatgaa aaaatggcat tatatatttt gcataattct
     4201 ctttcattta gggttaccgt gcgggtatgc ggcaaatgat ggcacgtgtg caacaagagg
     4261 cggcacacat acattaagcc ttaattttcc tctgacaacg gtcagtgcag caaacaatgt
     4321 gcctggaaat acattaatag atattgctaa tgcaacatct tctgaaaatt atagcgttct
     4381 gtgtaactgt gattcaaaac atagcaatgg cgcttatcac gaaatatatt ataccgcaga
     4441 ccctgctccc ggtatggttt atagcaccac cgcaagtggt cttgcttttt actatcttaa
     4501 cgaatatgtc gatgtgggaa caaaaatatc tgtgctaaat gcggggtata cggcagttcc
     4561 ttttgaacat gtttccaacc aggcaactac aacagatcac acttgtcagg gaaacaaaac
     4621 tacagcggtt ggcgtgagcc tgaaaactgg agcagatgcg aagatttcat ttcgtattaa
     4681 acgttcaata aatggaacgg tagtaatacc tatcaccgat attgcattgc tgtatgccaa
     4741 catatccagc accacgaccc gtggtgaggc gattgcaaaa gttcgaattt caggcagttt
     4801 gaccgcacca cagtcttgtc agataaatgc aggacaggtg atttattttg attttgatac
     4861 tattcctgcg tccgaatttt catctaccgc cgggcaagcc attacttcac gaaaaatcac
     4921 taaaacagtg agtattgagt gtacggggat ggggtatgag cgtacgcaga aagtcgatgc
     4981 ttcttttacg gggacgaacc gaagcagtga cgatacgatg gtggcgacag acaatgctga
     5041 tgtcgggatc aaaatttaca ataaatcgaa tgctgaagtt agcgtcaaca acggcaagtt
     5101 acccgcagac atgggcaaca cgaccatttt tggtcgtaaa aatggttcgg taactttttc
     5161 ggcagcacct gccagcttta ccggtgcccg gcctcagccc ggcgttttta acgctaccgc
     5221 gaccttaacc attgaatttg taaactaacc ttttgcggct gttttatgac agccgttcat
     5281 atttcctctc ctttcatgaa aaaatgctga tatttttccc cttcaatatc aataagtaat
     5341 aactttcctt tgggtgtcat caaactgtca cattccttac atataactgt cacctgtttg
     5401 tcctattttg cttctcgtag ccaacaaaca atgctttatg aatcctccca ggagacatta
     5461 tgaaagttat gcgtaccacc gtcgcaactg ttgtcgccgc gaccttatcg atgagtgctt
     5521 tctctgtgtt tgcagaagca agcctgacag gtgcaggtgc aaccttccct gcgccggtgt
     5581 atgccaaatg ggctgacact taccagaaag aaaccggtaa taaagttaat taccagggta
     5641 tcggttcttc cggtggcgta aaacagatta tcgccaatac cgttgatttt ggtgcttctg
     5701 acgcgccgct gtctgacgaa aaactggctc aggaaggtct gttccagttc ccgaccgtga
     5761 ttggcggcgt ggtgctggcg gttaacattc caggtctgaa gtctggtgag ctggtgctgg
     5821 atggtaaaac cctcggcgat atctacctgg gcaaaatcaa gaagtgggat gatgaagcca
     5881 tcgccaaatt gaatccgggt ctgaaactgc cttcacagaa cattgctgta gtgcgccgcg
     5941 cagatggctc cgggacttcc ttcgtcttca ccagctacct ggcgaaagtg aatgaagagt
     6001 ggaaaaacaa cgttggtact ggctctaccg taaaatggcc gatcggtctg ggtggtaaag
     6061 gtaacgacgg tatcgctgcg ttcgttcagc gtctgccggg tgcaattggt tacgttgaat
     6121 atgcttatgc gaagcaaaac aacctggcgt acaccaaact gatctccgct gatggtaaac
     6181 ctgttttgcc gaccgaagaa aacttcgcta atgcagcaaa aggtgcagac tggagcaaaa
     6241 ccttcgctca ggatctgacc aaccagaaag gcgaagatgc gtggcctatt acctctacca
     6301 cgttcattct gatccacaaa gatcagaaga aaccagaaca aggcacagaa gtgctgaaat
     6361 tcttcgactg ggcgtacaaa accggggcta aacaggcgaa cgacctggat tacgccagcc
     6421 tgccggatag cgtagttgaa caggttcgcg ctgcgtggaa gaccaatatt aaagacagta
     6481 gcggcaagcc gctgtactaa taaaactcca ggccggataa ggcattaaac ggcttatccg
     6541 gcattacaaa atgactttgt aaacgcgttt aactgaagag taacttatgg ctgcaaccaa
     6601 gcctgctttt aacccaccgg gtaaaaaggg cgacataatt ttcagcgtgc tggtaaaact
     6661 ggcggcgctg attgtgctat tgatgttggg tggcattatt gtctctctga tcatctcctc
     6721 atggccgagc attcagaaat ttggtctggc tttcctgtgg accaaagagt gggatgcgcc
     6781 gaacgatatc tacggggcgc tggtgccgat ctacggtacg ttggtgactt cgtttatcgc
     6841 gctgctgatc gccgtcccgg tgagtttcgg tatcgccctg ttcctgactg agcttgcgcc
     6901 tggctggctg aaacgcccgc tgggtatcgc cattgagctg ctggcagcca ttccaagtat
     6961 cgtttacggc atgtggggcc tgtttatctt tgcgccgctg ttcgccgttt acttccagga
     7021 gccggtcggc aatatcatgt cgaatatccc gattgtcggc gcgctgttct ctggcccggc
     7081 atttggtatc ggtatcctcg cggcaggcgt gatcctcgcc atcatgatta ttccgtacat
     7141 tgcggcggta atgcgcgatg tgttcgaaca aaccccggtg atgatgaaag agtcggccta
     7201 cggtattggc tgcaccacct gggaggttat ctggcgtatc gttcttccgt tcaccaaaaa
     7261 tggtgttatc ggcggcatta tgctggggct gggccgcgcg cttggtgaaa ccatggcggt
     7321 gacctttatt atcggtaaca cctaccagct cgacagcgcc tcgctgtaca tgccgggcaa
     7381 cagtatcacc tctgcgctgg cgaacgaatt tgccgaagcg gaatccggtc tgcacgttgc
     7441 cgcactgatg gaactgggcc tgatcctgtt tgtgattacc ttcatcgtcc tcgccgcatc
     7501 gaagtttatg attatgcgcc tggctaagaa tgagggggct cgctaatggc tatggttgaa
     7561 atgcaaacca ctgcggcgct ggcggaatcc cgccgcaaaa tgcaggcgcg tcgccgcctc
     7621 aaaaaccgta ttgcactgac gctctcaatg gcgacgatgg ccttcggtct gttctggctg
     7681 atctggattt taatgtccac catcacccgc ggtatcgacg gtatgtcgct ggcgctgttc
     7741 actgaaatga cgccgccgcc caatacggaa ggtggtggtc tggcgaacgc tctggcaggt
     7801 agcgggctgt taattttgtg ggccacggta ttcggtacgc cgctgggcat tatggcgggg
     7861 atttatctgg ctgaatatgg tcgtaaatcc tggctggcag aagtgattcg cttcattaac
     7921 gacattctgc tctctgcgcc gtcgattgtg gttggtctgt ttgtttacac catcgtggtg
     7981 gcgcagatgg agcacttctc cggttgggcg ggtgtgattg cgctggcgtt gttgcaggtg
     8041 ccgattgtta tccgcaccac cgagaacatg ctgaaactgg tgccggacag cctgcgtgaa
     8101 gcggcttatg cgctgggtac accgaagtgg aagatgatct ccgcgattac gctgaaagcg
     8161 tcggtgtccg ggatcatgac cggtatcctg ctggcgattg cccgtattgc tggtgaaacc
     8221 gcgccgctgc tgtttaccgc gctctccaac cagttctgga gcacggacat gatgcagccg
     8281 atcgccaacc tgccggtgac gatctttaag tttgcgatga gcccgtttgc ggaatggcag
     8341 caactggcct gggccggggt attgatcatt accctgtgcg tactgctgct gaacattctg
     8401 gcgcgcgttg ttttcgcgaa gaataaacac ggttgatatt gctgacacgg ttttcccctc
     8461 accctaaccc tctccccaga ggggcgaggg gaccgaccga gcgccttttt gactctgtac
     8521 acggttaaca ctttgccgga tgcggcgtga acgcctgatc cggcctacgg taagcctgat
     8581 tcgcgaagtg catcaggcac gatgaggaaa agattgcaat gagtatggtt gaaactgccc
     8641 cgagtaaaat tcaggttcgt aatttgaact tctactacgg caaattccat gccctgaaaa
     8701 acatcaacct ggatatcgcc aaaaaccagg taacggcgtt tatcgggccg tccggctgcg
     8761 gtaaatcgac gctgctgcgt accttcaaca aaatgtttga actgtacccg gaacagcgtg
     8821 cggaaggtga aattctgctc gatggcgaca acatcctgac caactctcag gatatcgccc
     8881 tgctgcgtgc gaaagtgggc atggtgttcc agaaaccgac gccgttcccg atgtccatct
     8941 acgacaacat cgcctttggc gttcgtctgt ttgagaagct ctctcgtgcc gacatggacg
     9001 agcgcgtgca gtgggcattg accaaagccg cattgtggaa cgaaaccaaa gataaattgc
     9061 atcagagcgg ttactctctc tctggtggtc agcaacagcg tctgtgcatt gcgcgtggta
     9121 tcgccattcg cccggaagtg ctgctgctcg acgaaccatg ttcggcgctc gaccccatct
     9181 ccaccgggcg tattgaagag ctgatcaccg aactgaagca ggattacacc gtggtgatcg
     9241 tcacccacaa catgcagcag gctgcgcgtt gttccgacca cacggcgttt atgtacctgg
     9301 gcgaattgat tgagttcagc aacacggacg atctgttcac caagccagcg aagaaacaaa
     9361 cagaagacta catcaccggt cgttacggtt gattcaggag tgcgttatgg acagtctcaa
     9421 tcttaataaa catatttccg gccagttcaa cgccgaactg gaaagtatcc gcacgcaggt
     9481 gatgaccatg ggcggcatgg tggagcagca gctttctgat gcaatcaccg cgatgcataa
     9541 ccaggacagc gatctggcga agcgcgtgat cgaaggcgac aagaacgtca acatgatgga
     9601 agtggcgatc gatgaagcct gcgtgcgcat tattgccaaa cgccagccga cggcgagcga
     9661 cctgcgattg gttatggtga tcagtaaaac cattgccgaa ctggagcgta ttggcgacgt
     9721 ggcggacaaa atctgccgta ctgcgctgga gaaattctcc cagcagcatc agccgttgct
     9781 ggtaagtttg gagtcgctgg gccgtcatac tatccagatg ctgcacgacg tgctggacgc
     9841 gttcgcgcgg atggacattg acgaagcggt acgtatttat cgtgaagata aaaaagtcga
     9901 tcaggagtac gaaggtattg ttcgtcaact gatgacctac atgatggaag attcgcgcac
     9961 cattccgagt gtacttaccg cgctgttctg tgcgcgttct atcgaacgta ttggcgaccg
    10021 ctgccagaat atttgtgagt ttatcttcta ctacgtgaag ggtcaggatt tccgtcatgt
    10081 aggcggtgat gagctggata aactgctggc gggaaaagac cccaaagagt agtgctaact
    10141 gtaggccaga taaagctttt tgccgcatct ggcaatcagc ccggtcatac atagttaccg
    10201 ggctgataca ctttctcact gaaatattgc cacacaaact cacctcgaaa ctctccccag
    10261 tacattgaat atacattatt tcatttgaat tattttactt ttaatgtagt gcctcgctat
    10321 aaaaacatat ttgttattct tttcatttat atcgctacct gcacagagaa tggaaaagaa
    10381 aacaaaaaac agggtaaaag ccctatatcg agacataaaa aatgagcgat tttttacagt
    10441 attcataggt ggtttagtca taatcgtagc aaaccaacac ccaggtatgc tttttaagac
    10501 aaatcggcaa tgtattatgc tcgggcgaat aaatattgtg ttcaggagaa atgattatgg
    10561 ctata
//
LOCUS       DAZFBR010000141        10397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFBR010000141 DAZFBR010000000
VERSION     DAZFBR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.141"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            183..758
                     /gene="ssuE"
                     /locus_tag="ACHJZ2_004236"
     CDS             183..758
                     /gene="ssuE"
                     /locus_tag="ACHJZ2_004236"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO4570238.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            751..1710
                     /gene="ssuA"
                     /locus_tag="ACHJZ2_004237"
     CDS             751..1710
                     /gene="ssuA"
                     /locus_tag="ACHJZ2_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO4570239.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWHPTQLEGKQL"
     gene            1707..2852
                     /gene="ssuD"
                     /locus_tag="ACHJZ2_004238"
     CDS             1707..2852
                     /gene="ssuD"
                     /locus_tag="ACHJZ2_004238"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO4570240.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            2864..3655
                     /gene="ssuC"
                     /locus_tag="ACHJZ2_004239"
     CDS             2864..3655
                     /gene="ssuC"
                     /locus_tag="ACHJZ2_004239"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO4570241.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVTVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3652..4419
                     /gene="ssuB"
                     /locus_tag="ACHJZ2_004240"
     CDS             3652..4419
                     /gene="ssuB"
                     /locus_tag="ACHJZ2_004240"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO4570242.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4462..7074)
                     /gene="pepN"
                     /locus_tag="ACHJZ2_004241"
     CDS             complement(4462..7074)
                     /gene="pepN"
                     /locus_tag="ACHJZ2_004241"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO4570243.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7340..8542
                     /gene="pncB"
                     /locus_tag="ACHJZ2_004242"
     CDS             7340..8542
                     /gene="pncB"
                     /locus_tag="ACHJZ2_004242"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO4570244.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8711..10111
                     /gene="asnS"
                     /locus_tag="ACHJZ2_004243"
     CDS             8711..10111
                     /gene="asnS"
                     /locus_tag="ACHJZ2_004243"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO4570245.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2347 a         2654 c         2798 g         2598 t
ORIGIN      
        1 gattcaacat ctaatgacta tccaataaat aaatatgcgt gatatataaa accctttctt
       61 tagtttattt tcagaaaaag atacacaaaa tttatatttg gcattttctt gtctctccga
      121 cagactgagc gaattgttat caatgttaac aaaacaagaa caattggtta taaggagaga
      181 gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc ttctcgctcc agctccttgc
      241 tggaatatgc gcgggaaaaa ctaaatggcc tggatgttga ggtttatcac tggaatctgc
      301 aaaacttcgc cgcggaagat ctgctttatg ctcgtttcga tagtccggca ctccatacct
      361 tcaccgaaca gttgcaacag gctgacgggc tgattgtcgc cacgcctgtg tataaagccg
      421 cttattccgg tgcgctgaaa accctgctcg acctgctgcc agaacgtgcg ttgcaaggca
      481 aagtggtgct accgctggcg acgggcggta cggtggccca tctgctggcg gtcgattatg
      541 cccttaagcc agttttaagt gcgctgaaag cccaggagat cctgcacggc gtgtttgccg
      601 atgactcaca agtaattgat taccatcaca aaccccagtt cacgccgaat ttacaaaccc
      661 gtcttgatac cgcgctagaa actttctggc aggcattgca ccgccgcgat gttcaggttc
      721 ctgatcttca ttctctgcga ggtaatgccc atgcgtaaca tcattaaact ggcgctggtg
      781 ggattgctta gcgtctctac gattgtggtt gctgcagaat cctcgcctga agcgttacgt
      841 ataggctatc agaaaggcag tattggtatg gtattggcaa aaagccacca gttactggaa
      901 aaacgctatc cgcaatcaaa aatctcgtgg gtggagttcc ccgctggccc gcaaatgctt
      961 gaggcgttaa acgttggcag tattgatctc ggcagtaccg gggatattcc gccaatcttc
     1021 gcccaggctg ccggggctga tttgctgtac gtgggcgtcg agccgccgaa gccaaaagcc
     1081 gaggtgattc tggtggcaga aaacagcccg atcaaaaccg cagccgatct taaaggccac
     1141 aaagttgcgt tccagaaagg ttccagttca cacaatcttt tactgcgcgc actacgccag
     1201 gccggactta agtttactga catccagccc acttatctga cgccagctga tgcccgcgcc
     1261 gcgttccagc aaggtaacgt tgacgcctgg gctatctggg atccctacta ctccgctgca
     1321 ttattacagg gcggcgtgcg ggtgttgaaa gacggcaccg atctcaatca aaccggatcg
     1381 ttttatctgg cagctcgccc ctatgcagaa aaaaacggcg cttttattca gggtgtactg
     1441 gcaaccttta gtgaggccga tgcgttaacc cgcagccagc gcgagcaaag tatcgcttta
     1501 ctggcaaaaa cgatgggctt accggcaccg gtgattgcct cttacttaga tcatcgccct
     1561 cctaccacca tcaaaccggt taacgccgag gttgccgcct tacagcagca aacggcagat
     1621 ctgttttatg aaaatcgtct ggtgccgaaa aaagtcgata ttcgccaacg catctggcat
     1681 cccactcaac tggaaggaaa acaattatga gtctgaatat gttctggttt ttaccgaccc
     1741 atggtgacgg gcattatctg ggaacggaag aaggttcacg cccggttgat cacggttatc
     1801 tgcaacaaat tgcgcaagcg gcggatcgtc ttggctatac cggtgtgctg atcccaacgg
     1861 ggcgctcctg cgaagatgcg tggctggttg ccgcatcgat gatcccggtg acgcagcggc
     1921 tgaagtttct tgtcgccctg cgtcccagcg taacctcacc taccgttgcc gcccgccagg
     1981 ccgccacgct tgaccgtctc tcaaatggac gtgcgttgtt taacctggtc acaggcagcg
     2041 atccacaaga actggcaggc gacggcgtgt tccttgatca tagtgagcgc tacgaagcct
     2101 cggcggaatt tacccaggtc tggcggcgtt tattgctggg ggaaaccgtc gatttcaacg
     2161 gtaaacatat tcatgtgcgg ggagccaaac tgctcttccc gccgattcaa cagccgtatc
     2221 ctccgcttta ctttggcgga tcgtcagacg tcgcccagga gctggcggca gaacaggttg
     2281 atctctacct cacctggggc gaaccgccgg aactggttaa agagaaaatc gaacaagtgc
     2341 gggcgaaagc tgccgcgcat ggacgcaaaa ttcgtttcgg cattcgtctg catgtgattg
     2401 ttcgtgaaac taacgacgaa gcgtggcagg ccgccgagcg gttaatctcg catcttgatg
     2461 atgagactat cgccaaagca caagccgcat tcgcccgcac ggattccgtg gggcaacagc
     2521 gaatggcagc gctacataac ggcaagcgcg acaatctgga gatcagcccc aatttatggg
     2581 cgggcgttgg cttagtgcgc ggcggtgccg ggacggcgct ggtgggcgat ggtcctacgg
     2641 tcgctgcgcg aatcaacgaa tatgccgcgc ttggcatcga cagctttgtg ctttcgggct
     2701 atccgcatct ggaagaagcg tatcgggtcg gcgagttgct gttcccacat ctggatgtcg
     2761 ccattccgga aattccccag ccgcagccgc tgaatccgca aggcgaagcg gtggagaatg
     2821 attttatccc tcgcagagtc gcgcaaagct aaggaagaag ataatggcaa cgccagtgaa
     2881 gaagtggtta ttgcgcgttg ccccctggtt tttaccggtg ggcatcgtga cggtgtggca
     2941 actggcctcc tcggttggct ggttgtcgac gcgtattttg ccctcaccgg aaggggtggt
     3001 gatggcgttc tggacgctct cagccagcgg cgaattgtgg cagcatctgg cgatcagctc
     3061 ctggcgggcg ctgattggtt tttcaattgg cggctcgctg gggttgattt tgggactgat
     3121 tagcggactg tcgcgctggg gagaaaggct gctggatacc tcaattcaga tgttgcgcaa
     3181 cgtgccgcat ctggcgctga tcccgctggt gattttgtgg tttggcattg atgaatccgc
     3241 aaaaatcttt ctggtggcgc tcggtacgct attccccatt tatatcaaca cctggcatgg
     3301 catccgtaat atcgatcgcg ggttggtgga gatggcgcgt agctatggat tatccggcat
     3361 accgctgttt atccatgtga tcctgcctgg tgccctgccc tcaattatgg tcggcgtgcg
     3421 ttttgcgttg ggcctgatgt ggctgacgct gattgttgcc gaaaccattt ctgccaattc
     3481 aggcattggt tatctggcga tgaatgcgcg ggagtttttg caaacggacg tggtggtagt
     3541 cgctattatt ctttacgccc tgctcggcaa acttgctgac gtcagcgcgc agctgttaga
     3601 gcgcctctgg ttgcgctgga acccggctta tcatttgaag gaggccacgg tatgaatact
     3661 gctcgtctga accagggcac accattgttg ctcaatgcag taagcaaaca ttacgcggaa
     3721 aatatcgtcc tgaaccaact ggatttacat attccggcag gtcagtttgt ggcggtggtg
     3781 ggccgcagcg gtggtggcaa aagtaccctg ctgcgcctgc tggcaggtct ggaaacgcca
     3841 accgctggcg atgtgttagc gggcaccaca ccgctggctg aaattcagga agacacgcga
     3901 atgatgtttc aggatgcgcg tctgctaccg tggaaatcgg tgattgataa cgttgggtta
     3961 ggccttaaag gccagtggcg cgatgccgcg cgtcgggcgt tggctgcggt aggactggag
     4021 aatcgcgccg gggaatggcc tgccgcactt tctggcgggc agaaacagcg agtggcgctg
     4081 gcaagagcgt tgattcatcg accgggttta ttgttgcttg atgaaccgct cggtgcgctg
     4141 gatgccttaa cgcgactcga gatgcaggat ttgattgtgt cactttggca ggagcacggt
     4201 tttaccgtac tgttggtgac gcatgatgtg agcgaagcgg tagcaatggc tgatcgggtg
     4261 ttgttaattg aagagggaaa aattggtctg gatttgacgg tggatattcc ccgtccgcgc
     4321 cggttagggt cggtgcgatt agcggagctg gaagcggaag tgttgcaacg ggtgatgcag
     4381 cgtggtgaat ctgaaactcg cctgagaaaa caggggtaaa atccccgaat ggcggcgcta
     4441 ttgccgccat tcggggattt attaagccag tgctttggtg atcttctcgt acagatcgcc
     4501 agatagattt tccagccctt tcaactgttc cagcgccgcg cgcattttct cctgacgttt
     4561 ggcatcataa cgtttcaggc gaatcagcgg ttcaatcagg cgtgaagcta cctgcgggtt
     4621 acggctgttc aggtcggtaa gcatttccac caggaactgg taaccgctcc catcttcggc
     4681 atggaacgct gccgggttgc tgcccgcaaa cgcaccaatc aacgaacgaa tgcggttcgg
     4741 gttgctcatg gtaaatgagc gatgctgcaa caggccgcgc accgtctcca gcacattcgc
     4801 cgccgggctg gtggcttgca ggataaacca tttatccatc accagaccat cctgatgcca
     4861 cttgtcgtcg tactcctgca tcagcgcgtc acggcaaggc agctgtgcag caaccgccgc
     4921 agaaagcgct gccagcgcat cggtcatatt gtttgcttca tggtattgct tgctcaccag
     4981 cacgtcagcc agatgcgttt caccaaaagc aaggaagcgc aggcaggcat tacgcagagt
     5041 gcgttttgca atatcttcat gctcaacacg gtactcgctc tggtaattcg cgttgtaaat
     5101 agccagtagc tcatccgcca gttcagtcgc cagagtacga gtgagtgctt cgcgtacttc
     5161 ggcaatagca atcgggtcga tgatatcgaa cagttcagcc atttcattga cagaaggcag
     5221 cgtcaggatt tctgccgcca gcgccggatc aatcttctca tcgagcagca ccgcgcggaa
     5281 agcgtcagcc acatgtaccg gcagagacag cggctgccct tgctgatgac gggcgacgtt
     5341 cagcttgatg taggttgcca gcaaactttg cgccgcatcc caacgggaaa aatcattacg
     5401 cgcatgacgc atcaggaagg tcagttgctg atcgctccac ttatattcca gtttcactgg
     5461 cgcagagaat tcgcacagca gcgcaggcac cggctggaag tagacattat caaaaacaaa
     5521 ggtctgttcc gcctgggtga cgttcagcac ggaattcacc ggatgaccgc ctttctgcaa
     5581 cgggatcact ttgccttcgt tgtcatacaa ttcgatggca aacggaatat gcagcggctg
     5641 tttttctgct tgatccggcg tggctggcgt gcgctggctg atggtcaggg tgtactgctc
     5701 ggtttccgga ttgtagtcgt ctttgacggt cacaaccggc gtaccggact ggctgtacca
     5761 acggcggaaa tgggagagat cgacattcga cgcatcttcc atcgcctgaa cgaaatcgtc
     5821 gcaggtcgct gcactgccat catggcgctc gaaatagagc tgcatccctt tctggaagtt
     5881 ttcttcgcca agcagagtgt ggatcatacg aatcacttcc gcgcccttct cgtaaacggt
     5941 cagggtgtag aaattgttca tctcaatgac catgtccggg cggattgggt gcgccatcgg
     6001 gctggcgtct tccgcaaact gcaatccgcg catggtgcgc acattattga tacggtttac
     6061 cgcgcgggaa ccaaggtcag agctgaattc ctgatcgcgg aagacggtta aaccttcttt
     6121 caggctgagc tggaaccagt cgcggcaggt cactcggtta ccggtccagt tatggaaata
     6181 ttcatggccg ataacgcgtt caatatcgag gtaatctttg tcggtggcgg tatcggtgcg
     6241 ggccagcaca tatttggagt taaagatatt cagaccctta ttctccattg cgcccatatt
     6301 gaagaaatcc accgcgacga tcatatagat gtcgaggtca tactccaggc caaagcgttc
     6361 ttcatcccat ttcatggagt ttttcagcga ggtcatcgcc cacggcgcgc gatcaaggtt
     6421 gccgcgatcg acgtacagct ccagtgctac ttcgcgacca gaacgcgtgg taaaggtatc
     6481 gcgcagtaca tcaaagtcgc ctgccaccag cgcaaacagg tagcacggtt tcgggaacgg
     6541 gtcctgccac tgtacccaat ggcgtccgtt ttccagttcg ccttgcgcaa cgcggttacc
     6601 gttggaaagc aggaagggat atttggtttt atcggcaata attttggtgg taaaacgcgc
     6661 cagcacgtcc gggcggtcga gataatacgt aatatggcgg aaaccttcgg cttcacactg
     6721 ggtgcaaagc gcatcgcctg actgataaag cccttccagc gccgtattcg ccgccgggct
     6781 tatttcatta atgatcttaa gcgtaaaacg ctccggcaaa ttactgatga ccagtgcgcc
     6841 ctcttcttct ttccaggcgg tccacggctc atcattaata tgaacagaaa ccagtttgag
     6901 gtcttcgccg ttgagacaaa gcggagcatc tgatgcacca tgacggacag cctggctgac
     6961 cgcggtaacg accgtctttt gcgcgtcgag gtcaaaggtc aagtcaatat cagtaatctg
     7021 gtaatccggc gcacgataat cgtgacggta tttggcttgt ggctgttgag tcataaataa
     7081 cctttagcat cttttataga gtctggtgtt cagtctattc ctgttgcgta aatcgcgcta
     7141 tgcagaatct tcatcttttc aggtacaaac gcctttattg ctacattttt ataacataca
     7201 ccgcgtaatg ccatcgacca gaaaggtggc atatggtgtg atcggggttc aataaattgc
     7261 gaaacaaggt atactccagc agttcctgaa gatgtttatt gtactaaacg ctcctgtacg
     7321 aggacgctac tgcgcaccta tgacacaatt cgcttctcct gttctgcact cgttgctgga
     7381 tacagatgct tataagttgc atatgcagca agccgtgttt catcattatt acgatgtgca
     7441 tgtcgcggcg gagtttcgtt gccgaggtga cgatctgctg ggtatttatg ccgatgctat
     7501 tcgtgaacag gttcaggcga tgcagcacct gcgcctgcag gatgatgaat atcagtggct
     7561 ttctgccctg cctttcttta aggccgacta tcttaactgg ttacgcgagt tccgctttaa
     7621 cccggaacaa gtcaccgtgt ccaacgataa tggcaagctg gatattcgtt taagcggccc
     7681 gtggcgtgaa gtcatcctct gggaggttcc tttgctggcg gttatcagtg aaatggtaca
     7741 tcgctatcgc tcaccgcagg ccgacgttgc gcaagccctc gacacgctgg aaagcaaatt
     7801 agtcgacttc tcggcgttaa ccgctggtct tgatatgtcg cgcttccatc tgatggattt
     7861 tggcacccgt cgccgttttt ctcgcgaagt acaagaaacc atcgttaagc gtctgcaaca
     7921 ggaatcctgg ttcgtgggca ccagcaacta cgatctggcg cgtcggcttt ccctcacgcc
     7981 gatgggaaca caggcacacg aatggttcca ggcacatcag caaatcagcc cggatctagc
     8041 caacagccag cgagctgcac ttgctgcctg gctggaagag tatcccgacc aacttggcat
     8101 tgcattaacc gactgcatca ctatggatgc tttcctgcgt gatttcggtg tcgagttcgc
     8161 tagtcggtat cagggcctgc gtcatgactc tggcgacccg gttgaatggg gtgaaaaagc
     8221 cattgcacat tatgaaaagc tggaaattga tccacagagt aaaacgctgg ttttctctga
     8281 caatctggat ttacgcaaag cggttgagct ataccgccac ttctcttccc gcgtgcaatt
     8341 aagttttggt attgggactc gcctgacctg cgatatcccc caggtaaaac ccctgaatat
     8401 tgtgattaag ttggtagagt gtaacggtaa accggtggcg aaactttctg acagccctgg
     8461 caaaactatc tgccatgata aagcgtttgt tcgggcgctg cgcaaagcgt tcgaccttcc
     8521 gcatattaaa aaagccagtt aatatcatca gggagctaat cggctccctt tttttacctt
     8581 taattccgaa atctttcgct gcatttgcga attctgcttg tctgattgca gatgccaggt
     8641 aacataggta tccccccatt tacgggtgga caagtgttta ttttttccga ctattaacag
     8701 agagaatatt atgagcgttg tgcctgtagc cgacgtactc cagggccgcg tagccgttga
     8761 cagcgaagtc accgtacgcg gatgggtacg tacccgccga gattcaaaag ctggcatctc
     8821 cttcctcgcc gtttatgacg gttcctgctt tgatcctgta caggctgtca tcaataattc
     8881 tctgcccaat tacaatgaag acgtcctgcg tctgaccacc ggctgctcgg tcattgtgac
     8941 gggtaaagtc gtggcgtcgc cgggccaggg gcaacaattt gaaattcaga ccagcaaggt
     9001 tgaagttgct ggttgggttg aagatccaga cacttacccg atggcggcaa aacgccacag
     9061 cattgagtat ctgcgtgaag tcgctcacct gcgtccgcgc acaaacctga ttggtgccgt
     9121 cgcgcgcgtt cgccatacgc tggcgcaggc gctgcatcgc ttctttaacg agcagggatt
     9181 cttctgggtt tcaacgccac tgattactgc gtctgatacc gaaggggctg gcgaaatgtt
     9241 ccgcgtttct acgctggatc tggaaaatct gccgcgtaac gatcagggca aagtggattt
     9301 cgacaaagac ttctttggta aagagtcttt cctgaccgta tctggccagt tgaacggcga
     9361 aacctacgct tgcgcattat ccaaaattta taccttcggc ccgactttcc gtgctgaaaa
     9421 ctccaacacc agccgtcacc tggcggaatt ctggatgctg gagccggaag tggcgtttgc
     9481 taacctgaac gatatcgcgg gtctggctga agccatgctg aaatatgtct tcaaagcggt
     9541 tctcgaagaa cgcgctgacg acatgaaatt cttcgctgaa cgcgtagata aagatgccgt
     9601 ttcacgtctg gaacgcttta ttgaggccga tttcgcgcag gtggattaca ccgaagcagt
     9661 aaccattctc gaaaactgcg gcaggaagtt tgaaaacccg gtttactggg gcgtcgatct
     9721 ctcttctgag catgagcgtt atctggcgga agaacacttt aaagcaccgg tagtggttaa
     9781 aaactatccg aaagatatta aagcgttcta tatgcgcctc aacgaagacg gtaaaaccgt
     9841 tgcggctatg gatgttctgg ctccgggcat cggtgagatc attggtggct cccagcgtga
     9901 agagcgtctg gacgtgctgg acgagcgtat gctggaaatg ggcctgaaca aagaagatta
     9961 ctggtggtat cgcgatctgc gtcgctacgg tactgttccg cattccggtt tcggtcttgg
    10021 ttttgaacgc ctgattgctt acgtaactgg tgtgcaaaac gtgcgtgatg tgattccgtt
    10081 cccacgaact ccgcgtaacg ccagcttcta atttagcgtc tacaagagcc agcacccgct
    10141 ggctcttttt tatcctttct gtcaagttat ctgtttgtta agtcaatcaa tctatttgca
    10201 accccgccat aacgatcatc ctgttacgga atattacatt gcaacattta cgcacaaaaa
    10261 ataacccgca ttcttattgc ggattagttt tttcttagct gatagcacaa ttttcatact
    10321 atttttttgg cattctggat gtctgaaaga ggattttggg ccaggtcgat aaagtttcca
    10381 tcagaaacaa aatttcc
//
LOCUS       DAZFBR010000142        10372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFBR010000142 DAZFBR010000000
VERSION     DAZFBR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.142"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            296..1189
                     /locus_tag="ACHJZ2_004244"
     CDS             296..1189
                     /locus_tag="ACHJZ2_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570246.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNESPKQSLLNDFSHRLKMEG
                     EEQLFHCVNELKYGLSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTFDVGEVINSYN
                     IDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAKLPQERS
                     EKVEDMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITNKYSAKDVAPDILV
                     LPVNVYHADFKIKINNAVEKT"
     gene            1196..2110
                     /locus_tag="ACHJZ2_004245"
     CDS             1196..2110
                     /locus_tag="ACHJZ2_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4570247.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEQPLFVKHTAMMREACKELPQLVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKKRMAELFNQSDTSVRNKYRPAFDKWNKLV
                     DKLNHERNTVPFACLTTIIATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            2804..3022
                     /locus_tag="ACHJZ2_004246"
     CDS             2804..3022
                     /locus_tag="ACHJZ2_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO4570248.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            3239..3481
                     /locus_tag="ACHJZ2_004247"
     CDS             3239..3481
                     /locus_tag="ACHJZ2_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO4570249.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3811..4740)
                     /gene="fdhE"
                     /locus_tag="ACHJZ2_004248"
     CDS             complement(3811..4740)
                     /gene="fdhE"
                     /locus_tag="ACHJZ2_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO4570250.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4737..5372)
                     /gene="fdoI"
                     /locus_tag="ACHJZ2_004249"
     CDS             complement(4737..5372)
                     /gene="fdoI"
                     /locus_tag="ACHJZ2_004249"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO4570251.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(5369..6271)
                     /gene="fdoH"
                     /locus_tag="ACHJZ2_004250"
     CDS             complement(5369..6271)
                     /gene="fdoH"
                     /locus_tag="ACHJZ2_004250"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO4570252.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            complement(6284..9334)
                     /gene="fdnG"
                     /locus_tag="ACHJZ2_004251"
     CDS             complement(6284..9334)
                     /gene="fdnG"
                     /locus_tag="ACHJZ2_004251"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8747..8749),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO4570253.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQGNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9528..10361
                     /gene="fdhD"
                     /locus_tag="ACHJZ2_004252"
     CDS             9528..10361
                     /gene="fdhD"
                     /locus_tag="ACHJZ2_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO4570254.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2640 a         2472 c         2623 g         2637 t
ORIGIN      
        1 cgtgtcttgc tacatcactg ttcaggagca tttaaatatt atttgtagag ctgtatagca
       61 attatcgcag ataatgctta attcatcatc ttagttcagg tttgagcgat ttttgatagt
      121 caaaaccccc tctggtgcca tagttttctc ataaatactt taaaaatcta tagctaacaa
      181 acattatgca aagataatgg gctggcatca ttgtcagtat cattcattta ctgctctgga
      241 tgttataaat aattatgtag catgtttgct cttaaaccgt gggaaggagg ctgtaatggt
      301 tgccaatatt aatctcatta aacaagaaag ttattctgtt gttaatttag aaaaacaact
      361 aatgagtaat gaatcaccga agcaatcgct attaaatgat ttttcacatc gccttaaaat
      421 ggaaggtgag gaacaactgt tccattgtgt taacgaactg aagtatggtt tgtcgagtaa
      481 taatgaatat gacctgttta aaaatagaga aacctcactg tggaaacgca aagtaccaga
      541 aagcgcagcg ctggcaatga cgggaacagt atataaatta tttggtagtc gcttactcaa
      601 cgcccgcaat cagttagata aaaatctcct cgaaatggct atgcaaatgg tttatggcaa
      661 agatatggtg acaaagtccg gggatatatt gattgatata caattaaata aggatggcag
      721 cctccagagt aagcactaca catttgatgt aggtgaagtt ataaacagtt ataatattga
      781 cctctttgtg aatgcttcga gtattaatca aacttactta catgataaaa atcctggtga
      841 tgttattagc ctgtatggca ctttagataa cattcctctt gtagttaagg aatgcacggg
      901 taaaaatcat caaattaacg ttaaagccaa attgccacaa gaacgaagtg aaaaagttga
      961 ggatatgtgt gaaagaatga gaggtggcat ctctatgttt aatcacacca caaaaacagc
     1021 tggtaatata gagcataatt tgcagctaag ttttctcgtg gattcacggc cgtcgatcac
     1081 caataaatac tcagccaaag atgtagcgcc agacatactt gtattgcccg tcaacgtata
     1141 tcatgcggat tttaaaatta aaatcaataa tgcagtagag aagacttagt caattgtgtt
     1201 atgtgctgtg caaaaaaatc tccatgcagc aaggaaggtt cttagcggga taataggtaa
     1261 tgtaccaaaa aataataaac caaaaacaat catttttgat ttacgttttc ttagtgcaat
     1321 taagcagaaa gtttttttgg gcagtgaaca accgttgttt gtaaagcata ccgcaatgat
     1381 gagggaggca tgtaaggaat taccgcagct agtggagtat gttcctttag cctgtgaagc
     1441 gcatagccat cgcagcgtcg cccttgccaa aaccgtggct gcacccatca tggcgacagg
     1501 tgtgggcgcg gctttaatgt atgcaacatc atcaggaccg tattatgcgc aaagtctttc
     1561 tggttcgccg gatatcgaga tgataaaaaa acgcatggct gaattgttta accagtcgga
     1621 tacttctgtg cgcaacaaat atcgcccggc ttttgataaa tggaataaac tggtagataa
     1681 attaaatcac gaaagaaata cagttccgtt tgcgtgtttg accaccatta tagcgacagc
     1741 gataaatgaa actcctgagg gggatacgaa tgttgccgtt gtgatggggt gtaaatctgc
     1801 gaaagatcgc acgatatcca ttgtccttgg caactcgatg ttgcaaacac tctttgaaaa
     1861 acgcctcgcc gatgatggag aaatagaaaa cctttttgat caacagggat attttagttg
     1921 cgattcatta acagccgaag aagtaatgat gttaaaagac ctttttgata ttcgagttct
     1981 tcatctctct aacaaattta atgttgggtt gcaaggtaat atcaacacgg atgttttaca
     2041 agattcgttt tttaaaaatg tcgatttcat tcatgaatct aactcgtata ccgtagggat
     2101 gggagtctga agagtaaaac tcattaggat tatcagaatt cggccaatgc attgcaaaaa
     2161 tgaaatacaa agagagatgt gtgcaggcgc ttagccgcga ggggcatggc gaagccatac
     2221 agaaagcacc ggcattcaga actcctgtga aagcggcata gtgccgctgc tcaggagttt
     2281 tgttatgacc cgtatcgtgc tgaacgttgc caccctatga tgcgattaaa aaaactcacc
     2341 gtcgagccga tgattgacgt caatgctatc ggatgatgcc gggggtgttc cggaaggaat
     2401 tccttgtttc ccgaactgtt accaacaaaa ttataacgat gagcaagtaa agaccggttg
     2461 gtggcagtac tgaccggtct gtgatggaag attaatttca ccttaaatcc tggtgctgtt
     2521 aggatgctga cgtttttcgg gtacaccatt cagattatgc aaataaagat tcaatgaaaa
     2581 aataaatatc cctgaatata atatattcgt agcacccata actcatcctg ggcgataact
     2641 aaaccatttt tgagtaacaa atgaaacaac tgcaacgctc ttattatttc tggaaaacgt
     2701 ctcgcaaaaa caaaacaaca tgcaaaaatc cgctttcgtt aattaatttt tcggcgtaat
     2761 tttcgcacat acattaatga gtaatatatg taaggtggtg gtaatggcga tgaatacggt
     2821 ttttcttcat ttatcagaag aggcaattaa acggctgaac aagcttcggg gctggcgtaa
     2881 ggtttcacga tctgcaattt tacgcgaagc ggtagagcaa tatctggaac gtcagcaatt
     2941 cccggtgcgt aaagcaaagg gaggcaggca aaagggggag gtagttggtg ttgatgatca
     3001 gtgtaaggag cataaggaat gacaaaaatt tttcattctt gaatataaaa aacagatgcc
     3061 cttattctgg tattaataga aggctgtttt acttgaactt ataataactg caactgttac
     3121 atcatatctg gaaaacgcct cgcaaaaata aaaaatgatg cgtaaatgag ctttctttaa
     3181 agattcgaca agggtacttt tctctccata gatatcaaat gatattggtc aaatatttat
     3241 gaactctctg gcaggtattg atatgggcag aattatactc gatttatcga atgaggtgat
     3301 taagcaactt gatgatcttg aggtgcagcg taatcttcct cgtacggagc tattacggga
     3361 agcggtagat caatacttgg taaatcaatc gcaaacagta agaaccagtg cttttggcat
     3421 atggcaaggg tgtgaggaag atggtgtcga ataccagcgt aagctgcgcg aggaatggta
     3481 atgggaaaaa atgatatcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc
     3541 ggcgtgaacg ccttacccgg cctacaaagt cgtataaatt caatgtattg cagtttattg
     3601 gtaggcctaa taagcgcggc gcatcaggca atcttacgct tatcatcagt ctcataccca
     3661 taaccttatt gatgtggggg tgggtaaatt gaggcaaaac aggcactggc ggcatagctt
     3721 gaggtggtaa cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg
     3781 cgaccagcgc cacccggaaa acgtcagaga ttactcccct tcacccggaa acaggaacgg
     3841 gttgatggaa ctgcgggcat agccttcttg ctccattcgc gcgtccagta ccagagaggc
     3901 gaggtcatct gccacggctt caactttcgg ttctttttcc tgatagagaa ttttcaggta
     3961 agtgccgcag tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca
     4021 gtaatgcagt ttgccgctct gctcgcagtt gctgcatttt acgcgcacaa cgtgccattc
     4081 agtttcacac aggttgcagt gcaggtaacg tagcccctga gtggtgccaa tttgcaccat
     4141 gctggacacc ggcatagaac cacaaaccgg gcaatattga cgttgctcgc cgtattcagc
     4201 gcgggctttg ccggggatca gattggccat ctgcgcccag tagagcgaca gtgcagccca
     4261 gataaacggc gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gtgcgctggc
     4321 catatcttcc agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg
     4381 gccgctcatt tcaggtttca gctcagcaat cagcgccatc agcagctttt gccagtgctt
     4441 atcacgcggc agaacgtgaa tatccagcgg gggcttgcct tgtgcgctgg cttctttaat
     4501 gcgcgcagtc agatccatct ccagcggatg gtcgtacagc accacttcct gggcgtgggc
     4561 gataagcgca gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag
     4621 acgctcggcg cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat
     4681 atccgccgta cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat
     4741 tcagctttct tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc
     4801 gcccaagcgc tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg
     4861 gcggcgtaga tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc
     4921 attaacgcga atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata
     4981 atcacgccgc tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc
     5041 tgaccgaagt tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata
     5101 ttcttcgccc aaaagatatc atcccgattg attaggttgt gatgccagta acggaaaaac
     5161 atgatgatga acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc
     5221 tgcggtgtgc ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg
     5281 ctcaccgccg ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg
     5341 gtgtagcgca cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca
     5401 gattattctc ttcctcatcc gcatggttcg gaccgacacc cacgtagtgg aagatactgg
     5461 ctgcgaaggt agccgcaaag ccaacagctg cgagcggttt ccagatgcct ttccagaatt
     5521 tcacggtttc gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag
     5581 catggtgcag cacgtacata acgtgtgtac caccgacgcc tgccggatcg tacaggcccg
     5641 cattgtcgta accgcgggtt ttcagctccg ccacgcgctc gctcgccagc gttttcatcg
     5701 actctttcgt accgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt
     5761 gcccaaccac cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt
     5821 tgaggcgcgg aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct
     5881 cggactggaa gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg
     5941 ctttcagaca gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca
     6001 gtttgtcgtt ctgctccact tccgagaagc gcatcaccgt ccacgatttg gcgcttaaat
     6061 cattggggtt gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact
     6121 ctgaacacgc cacctgacag gctttacagc cgatacaggt ggtgacgtcg atgagtttcg
     6181 ccacttcttc ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac
     6241 gacgaatgat atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat
     6301 tcacaaggaa ggacttaaac tccggcgtct gcgtgttcgc atcaccgacg aatggcgtca
     6361 acgtattggc aataaagcct tttttcgcaa caccttcata gccccagtga ataggaatac
     6421 cgatggtatc gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg
     6481 ctttggcttt gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc
     6541 caagtttatt cgccagcgac tccccgattt ccacaaactg ctctggttgc aaaatcgcgt
     6601 tcaacagcgc gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga
     6661 catacgggaa cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag
     6721 ccgggttcga gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct
     6781 cgtagtgttc cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc
     6841 cttcctgctg catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg
     6901 gaatatccca gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc
     6961 acgggttacc ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc
     7021 atgcccatgc ccaacccagc gtgttaccga ggccagacgg atcggcgtta tcacggcgtg
     7081 ccatctggtt gccttccggc gtccagctac cggcgaaaat ccagcaacca caggaagttg
     7141 taccgtcatc gcgcagttgg gcaaacgagc taagttgttg gcctttcttg acgataaccg
     7201 caccggttgc cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt
     7261 cttccgactt cggctcatgc ggaatggcgt agttccaggt catgttcagc acctggtccg
     7321 ggttcgcgcc accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga
     7381 tctcgccatc agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc
     7441 agcgacctga gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga
     7501 acacttcggt ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga
     7561 agttagaggt ttcagtgttc agcgggtcga tggtgacgag gaacttcagt ttcgacagac
     7621 aactgatcac tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat
     7681 agccattgac cttgccttct ttcatcatct cgaagtattg caggacgtcg tagcctttat
     7741 cccacttcgg caaccagtca aagccccagc tattttctgc cgtcgcttta tcaccaaaga
     7801 aggccttcat catagagacg aagaatttcg ggtagttgcc ccagtagttt ccctggcctt
     7861 ccagcagcgg ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc
     7921 ttggcagcgt catgtaacct ggcaggctct gcgacagcag ccccaggtcc gtcagcccct
     7981 gaatattgga gtgaccgcgc agggcgttaa cgccgccgcc agccatcccc atgttaccga
     8041 gcagcagctg gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc
     8101 agccgagggc atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt
     8161 cgcagacttt caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg
     8221 tgtagcggga aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg
     8281 tatcgcgttt ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact
     8341 tgcgtttttc cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca
     8401 cgatcaggct ggcgttggta taggcttcag tgtattcgcg gttgaatttt tcattgttca
     8461 gcaggtacag caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat
     8521 agtagtcagc caccgcagcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc
     8581 cattgtgaat tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt
     8641 taccgcccat caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg
     8701 caccgcgacc aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct
     8761 ggttgtcgac cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt
     8821 cgttactcga cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc
     8881 cttcggcgtt ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc
     8941 gatcaaacgc ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt
     9001 ccggaaactt cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac
     9061 aaagtgcacc gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt
     9121 tggcgttttt tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat
     9181 aggtgcaggt attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga
     9241 gtgctacgct gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc
     9301 agatcttaaa gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc
     9361 acgtcccatt tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg
     9421 aaggggccgc aaaggtccag aattggctca atttccctcc atccaggaag ggtttgtaac
     9481 agaataccat aatgttggtg tgtgtgttct tatctggtta agagaaagtg aaaaaaacac
     9541 agcaaaaaga aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc
     9601 ggcgtgatga tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc
     9661 tggtctacaa cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact
     9721 ttgcgctcgg tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca
     9781 tggatgtcgt tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct
     9841 ttatggggtt gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg
     9901 gcgtggagca acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt
     9961 ttgatctcaa caaactggat gatgcattac gtcatctcaa cgattttcag ccagtgggac
    10021 aactgaccgg ttgtactcac gccgccgcct ggatgttgcc atctggcgaa ctggtcggcg
    10081 ggcatgaaga cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag
    10141 aaggggaaag ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg
    10201 tgcaaaagtc ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc
    10261 ttgctgtaga agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc
    10321 gggcaacggt ttatacccat ccgcagcgtt tgagcaatta attaaaataa gt
//
LOCUS       DAZFBR010000143        10113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFBR010000143 DAZFBR010000000
VERSION     DAZFBR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.143"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            319..933
                     /gene="evgA"
                     /locus_tag="ACHJZ2_004253"
     CDS             319..933
                     /gene="evgA"
                     /locus_tag="ACHJZ2_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO4570255.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            938..4531
                     /gene="evgS"
                     /locus_tag="ACHJZ2_004254"
     CDS             938..4531
                     /gene="evgS"
                     /locus_tag="ACHJZ2_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO4570256.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPSIGTTFTITIPVEIIQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNVDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQNN"
     gene            complement(4587..5732)
                     /gene="yfdE"
                     /locus_tag="ACHJZ2_004255"
     CDS             complement(4587..5732)
                     /gene="yfdE"
                     /locus_tag="ACHJZ2_004255"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO4570257.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGLSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQTILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5806..6750)
                     /gene="yfdV"
                     /locus_tag="ACHJZ2_004256"
     CDS             complement(5806..6750)
                     /gene="yfdV"
                     /locus_tag="ACHJZ2_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO4570258.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6820..8514)
                     /gene="oxc"
                     /locus_tag="ACHJZ2_004257"
     CDS             complement(6820..8514)
                     /gene="oxc"
                     /locus_tag="ACHJZ2_004257"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO4570259.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8568..9818)
                     /gene="frc"
                     /locus_tag="ACHJZ2_004258"
     CDS             complement(8568..9818)
                     /gene="frc"
                     /locus_tag="ACHJZ2_004258"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO4570260.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2997 a         2275 c         2088 g         2753 t
ORIGIN      
        1 tggaacgttg gaggcctaat ttaaatgagg tgcaaaaaaa acaacagagg actaaaccgt
       61 ggcttttgca atacaattct tacgcctgta ggattagtga gaagacttat agtgctaact
      121 tgaaactata aatcatcggt ataatccctg attttattgt tgacatttca tatatgccga
      181 ctatttatat ggtatacttg tcgaattatc ttaaaggaag ctcaaatttt cttattttta
      241 ttgagaaaat gagatgatgc cttatgtctg tattactaca gggagaaggg agatgcttca
      301 ttgcaaaggg aataatctat gaacgcaata attattgatg accatcctct tgctatcgca
      361 gcaattcgta atttattgat caaaaacgat attgaaatct tagcagagtt gactgaaggc
      421 ggaagtgccg ttcagcgggt ggaaacactt aagcctgata tcgtcatcat tgatgtcgat
      481 atccccggag ttaacggtat ccaggtgtta gaaacgctga ggaagcgcca atatagcgga
      541 attattatta tcgtctccgc taaaaatgac catttttacg ggaaacattg tgctgatgct
      601 ggcgctaatg gtttcgtgag taaaaaagaa ggcatgaaca atatcattgc ggctattgaa
      661 gctgcaaaaa atggctactg ctatttcccc ttctctctca accggtttgt tggaagttta
      721 acgtccgacc agcaaaaact cgactcctta tcgaaacaag aaattagtgt catgcggtat
      781 attcttgatg gcaaggataa taatgacatt gctgaaaaaa tgttcatcag caacaaaact
      841 gtcagcactt ataaaagtcg cctgatggaa aaattagaat gtaaatcact gatggatctt
      901 tacacattcg cacaacgtaa caaaatcggc taaccacatg aagtttttac cctatatttt
      961 tcttctctgt tgtggtcttt ggtcgaccat aagtttcgca gacgaagatt acatcgaata
     1021 tcgtggcatc agtagtaaca accgtgtcac acttgatcca ctacgtctga gcaacaagga
     1081 attacgttgg ttagcgagca aaaaaaatct tgtgattgca gtacacaagt cccaaacggc
     1141 tacgttgttg cataccgatt cgcagcaacg ggttcgtggt attaatgctg attatttaaa
     1201 tcttttaaaa agagcgttaa atatcaaatt aacactccgg gaatacgcag atcatcaaaa
     1261 agcaatggac gcgctggaag aaggtgaagt cgatatagtg ttatcacatt tagttgcttc
     1321 gccgcctctt aatgatgaca ttgctgcaac caaaccactg ataattacct ttccggcgct
     1381 ggtcaccacc cttcacgatt caatgcgacc gcttacctca tcaaaaccag taaatattgc
     1441 tcgagtagca aattatcccc ctgacgaggt aattcatcaa tcatttccaa aagcaacaat
     1501 tatctctttt acaaatttat atcaggcatt agcatccgtc tcagccggac agaatgatta
     1561 ctttattggt agtaacatca ttaccagcag tatgatttcc cgctatttca ctcactcctt
     1621 aaatgtagtg aaatattata actcaccgcg tcaatataac ttcttcttaa ccagaaaaga
     1681 atctgtcatt cttaatgaag tactcaatcg atttgttgat gctttaacaa atgaagttcg
     1741 ctatgaagta tcacaaaatt ggcttgatac aggaaacctg gcctttctga acaagccatt
     1801 agaactcact gaacatgaaa aacagtggat taagcagcat cccgatttaa aggtgctgga
     1861 aaatccttac tcgcccccct attctatgac ggatgaaaat ggctcggttc ggggcgttat
     1921 gggggacatt cttaatatta ttaccttgca aacaggttta aatttttctc cgatcaccgt
     1981 ttcacacaat atccatgctg gaacacagct gaatcccggc ggatgggata tattacccgg
     2041 tgctatttat agtgaagata gagaaaataa tgttttattt gctgaagcct tcataacaac
     2101 gccttacgtt tttgtcatgc aaaaagcgcc tgacagtgaa caaacattaa aaaaaggaat
     2161 gaaagttgcc attccatatt attatgagct tcattcgcaa ttaaaagaga tgtatccgga
     2221 ggtagagtgg ataaaagtcg ataacgccag cgctgcattt cacaaggtca aggaaggcga
     2281 acttgatgct ctggtcgcga cacagttaaa ttcacgttac atgatcgacc attactatcc
     2341 taatgaactg tatcattttc ttattcccgg cgttccgaat gcatcgcttt cgttcgcttt
     2401 tcctcgcgga gaaccggaac ttaaggatat tattaataaa gcactgaatg caatcccccc
     2461 aagcgaagtt ctgcgcctga ccgaaaaatg gattaaaatg cccaatgtga ccattgacac
     2521 atgggacctc tatagcgagc aattttatat tgttacgaca ttatccgttt tattagttgg
     2581 cagtagcctt ttatggggat tctacctgtt acgctcagtt cgtcgtcgta aagtcattca
     2641 gggtgattta gaaaaccaaa tatcattccg gaaagcgctc tcggactcct taccgaatcc
     2701 aacttatgtt gtaaactggc aaggtaatgt cattagtcac aatagtgcat ttgaacatta
     2761 tttcactgct gattactaca aaaatgcaat gttgccatta gaaaacagtg aatcaccctt
     2821 taaagatgtt ttttctaata cgcatgaagt cacagcagag acgaaagaaa accgaacaat
     2881 atacacacag gtatttgaaa ttgataatgg catcgagaaa agatgcatta atcactggca
     2941 tacattatgt aatctgccag ccagcgaaca tgctgtttat atttgtggtt ggcaagatat
     3001 taccgagacg cgtgatttaa ttcatgcact cgaagtagag agaaataaag cgatcaatgc
     3061 aactgtcgca aaaagtcagt ttctggcaac aatgagtcac gaaataagaa caccaataag
     3121 ctccattatg ggcttcctgg aacttctgtc gggttctggt cttagcaagg agcaacgggt
     3181 ggaggcgatt tcactagcct acgccaccgg acaatcactc ctcggcttaa ttggtgaaat
     3241 ccttgatgtc gacaaaattg aatcgggtaa ctatcaactt caaccacaat gggtcgatat
     3301 ccctacttta gtccagaaca cttgtcactc tttcggtgcg attgctgcaa gcaaatcgat
     3361 cgcattaagt tgcagcagta catttcctga tcattacctg gttaagatcg accctcaggc
     3421 gtttaagcag gtcttatcaa atttactgag taatgctctc aaatttacca ccgagggggc
     3481 agtaaaaatt acgacctccc tggttcacat tgatgacaac cacgctgtaa tcaaaatgac
     3541 gattatggat tctggaagtg gattatcaca ggaagaacaa caacaactgt ttaaacgtta
     3601 tagccaaaca agtgcaggtc gtcagcaaac aggttctggt ttaggcttaa tgatctgcaa
     3661 agaattaatt aaaaacatgc agggtgattt gtcattagaa agtcatccaa gcataggaac
     3721 aacatttacg atcacaatcc cggtagaaat tatccaacaa gtggcggctg tcgaggcaaa
     3781 agcagaacaa cccatcacac tacctgaaaa gttgagcata ttaatcgcgg atgatcatcc
     3841 gaccaacagg ctattactca aacgccagct aaatctatta ggatatgatg ttgatgaagc
     3901 cactgatggt gtgcaagcgc tacacaaagt cagtatgcaa cattatgatc tgcttattac
     3961 tgacgttaat atgccgaatg tggatggttt tgagttgact cgcaaactcc gtgagcaaaa
     4021 ttcgtcctta cccatctggg ggcttacagc caacgcacag gctaacgaac gtgaaaaagg
     4081 gttaaattgc ggcatgaact tatgtttgtt caaaccgttg accctggatg tactgaaaac
     4141 acatttaagt cagttacacc aggttgcgca tattgtacct cagtatcgcc accttgatat
     4201 cgaggccctg aaaaataata cggcgaacga tctacaactg atgcaggaga ttctcatgac
     4261 tttccagcat gaaacacata aagatttacc cgctgcgttt catgcactag aagctggcga
     4321 taatagaact ttccatcagt gtattcatcg catccacggt gcggctaaca tcctgaattt
     4381 gcaaaagttg attaatatta gccatcagtt agaaataaca cctgtttcag atgacagtaa
     4441 gcctgaaatt cttcagttgc taaactctgt aaaagaacac attgcagagc tagaccagga
     4501 aattgctgtt ttctgtcaac aaaataacta aatagtggct cccacaatgt tcaaatgtgg
     4561 gagctattta ccagcacatc ttacatttat gatgagaact cctggcgaat ttgctcccca
     4621 tgctggtcga gcgttgccgc tcccggcata acatgcgggt ccgcgcagcc actgattttt
     4681 atcggattac ccggcatcat tattcccccg gcttcaatca acatatttct cgcctgagtt
     4741 tgtggcaaat taatggcctc agccacactt aataacggcg cgacgggcac accaacttca
     4801 tgtattctgg ctaaccaaac ttcagctgcc tgtgttttta acgtccgctc aatatattgt
     4861 ttaagaatag tctggttttg tacgcgtaaa atattgctgc taaatctggg atcattaacc
     4921 agttccgtaa gctccagtgc ctggcataac gcagagaaaa gcttgtcatt accacaacaa
     4981 atcgtaatcg gcttatcctg agtatcgaaa acatcaaaag gtgccatgta ggggtggcga
     5041 tttcccaggc gttgtggtga cttccctgtc gcaatatatg ccatcagacc atgctccaga
     5101 aaactcagcg tggcatcaaa catcgctata tcgacatgcg cccctctttg gctcttttcg
     5161 cggccataaa gtgcactcac tattccgctg aataaataaa caccgccgca tagatccgca
     5221 agagaggtac caacacgcac tggcggagca tcagggtacc ctgtttccat cattatcccg
     5281 ctcattgcct gaatgatggt atcgtaggca ggagcatctt ttagcggacc ggtatgtccg
     5341 aaacctgacg atgaagcata tatgaggcgc gggttgattt cttgtaacct ttcccatgaa
     5401 agccccagtt tttccattgt acctgggcga aaattctcag ctaatacatc agcttgttta
     5461 agcatattta taaatatact tttatcgtga tcattcttta aatcaagaac cacactctct
     5521 ttaccatgat taataaaact gtaatagagt gactgtccat ccacataggg accaaatgtg
     5581 cgggtatcat caccatgacc cggcggctca actttaatta cccttgcgcc catattacaa
     5641 agaagttgag ttccgaaagg tccattaagg acatgtgtca tatcgataac taataagcct
     5701 tcaaacggcc ctttgctttc attatttgtc atttgcttat gccttgaaaa aagccgaagg
     5761 tgaaaagaca cttcggcatt tatgtaaaat caataacatg attaattatg aaaccagtcg
     5821 actgacataa atccacaagg gagccgtgac gacaaaaccc agtacgctca cagccaatga
     5881 cgctgtacca gtgcgggtgt agacattaaa ccggctggca ataatgattc cggagaatgc
     5941 cggcggtaat gcgcctgcca gtaccatcat ctgcagatgt tcgctgttca aatgacatgc
     6001 cataccaaca agaagcagcg ccagtggcat cagaatcagc ttcaggaagg tgttataagc
     6061 aatttcggca ctgaactcga atttatgtgc agccagagtc aaccccgcag cgaataccgc
     6121 tacccctgag ttagccttcg caatcagatt aaaggttggg tcccatgctg ccggaatttt
     6181 taccccaacc aacaccagga tcgttgccag aacaggtgcc catacaactg gctcctttgc
     6241 cgcagaaatt aatgcgctca gattactatt cttcttacca tccgctcctg aggaaggatt
     6301 cagcaaatac agaccaatag gaatagtaat tgcgttaaca ataatagaaa taattgctac
     6361 cactaaacct gttgatacgg aatcaccata aataggatcg agaactgcaa accccaggaa
     6421 tccaatggta ggtgaacctg caattaatgc acatacagct gcttctgcat gggtacgttt
     6481 aaaaaatttg tagcaaccaa accaggagaa aaagaaacat ccgacaataa ccacgagtga
     6541 tacaagggtc agacgagtgt ccgcaaaaat catttccctg tttgcccggg taatagaaac
     6601 aaatagggct gcaggaagcg cgtagtttaa taccagttta ttaaatgccc gggcttgatc
     6661 ttctgaaaaa gtttctcgtc tgccgctaaa ataacccaac agcatgatta cgataatagg
     6721 caataaatcg ccaataaaaa atgttagcat aatagtattt ccgataaggg tcccctcacc
     6781 atcgcataat gagttggcaa gaggagacga tgtcagtatt taattaccag cgacttgttt
     6841 tgggttaagt ttggtaatat ggccactttc agttcctgct gcagggtcga tgaccacatt
     6901 aataatggtc ggtttgcgcg actgaatacc ggtggttaaa gcatgacgaa gttcatctgt
     6961 cgtggtgacg ttatagccaa cgccacgaaa cgcatccatt aatttgtcat accttgcatg
     7021 gtgcaacaga tctgttggtg atggtgcacc agcgccactg agatcaacac cgtctcctct
     7081 gtagatgccg ccattattaa aaataacgat cgttaccggc aggttatatc gacaaatcgt
     7141 ttcaatttcc atcccactga aaccaaaagc actatcacct tcaatggcga caaccggaga
     7201 accagaggtc acgctagcac cgatggcata gcccataccg atgcccatga caccccaggt
     7261 accacaatcc agacgacgac gtggtttata catatcaata atatttcgtg cattatccag
     7321 ggtatttgca ccttcattaa ctaaataaat atcctggttc tcacgcaata catcgcgcac
     7381 agcacttaat gcattaaagt aatttaatgg ttgggtatct gtacttaatt tttcatgcat
     7441 tttttgtgca ttttgctgct tgtggatatt taaaatatcg cgccatacca gtggagtcgt
     7501 aaatgtgttt tgtttcagct ctgccagcat accttgcata ctggatgcaa tatcgccaac
     7561 gactggcaca gcaatggggc ggttgctgtc aatttcctgc ggttcaatat ccagttgaat
     7621 aaactgtgta tctgccgccc atcctttttt accgtgtgcc agtaaccaat tcagtcgtgc
     7681 accaacaagc atgacaacgt cagcatttgc cagggcaaac gaacgcgcag ctgccgcaga
     7741 aagtggatgc gtatcttcga ggatcccttt cgccatagac attggcagga atggaatctg
     7801 ggcactttca ataaattcac gaagctgttc gtcagcttgt gaatacgccg cgcctttgcc
     7861 aaggataatt aatggccgtt cagcttttgc taaaagcgaa attgcgctag tgactgactt
     7921 cgggcatggc aataatgctg gcgacggatt ttcaactttt acaatcgtgg ttaacgcttc
     7981 gtctttttcc atcgtcgcgg ccaggacatt tgctggcaaa tcaagataaa ctccgccagg
     8041 gcgacccgat actgagaccc ggatagcgcg tgccaatgca atgccaagat cctgcggctg
     8101 attaacgcga aatgctgctt tggcatacgg ttttgccgca ttcatttggt ccagctcttc
     8161 ataatcacct tgctgtaggt cgacgatcgc gcggtcgctg gagccgctaa tcatgatcat
     8221 cggaaaacca tttaccgttg cgttggccaa tgcggtcaaa ccattgagga atcctggcgc
     8281 agacactgtc aggcagatcc ctggtttttg ggtaagaaaa ccgcttgccg cagcggcata
     8341 gcctgccgac tgctcatgac gaaaaccaat ataacgaatg ccttccgcct gggcatggcg
     8401 cgccatatcc gtcacaggaa tacctacaac accataaata gtgtcaatat tattctgttt
     8461 taatgcttca acgatgatat gcataccatc tgtcatttga agttgatctg acatgattaa
     8521 taacctcgaa atttttataa attgggagcc agaagccccc gttaatatca gatggcgtgg
     8581 ttttgcttca ttgcagcaat ttcatcgtcg ctataaccca gctcctgcaa taccgcagcg
     8641 gtatgttcac ctaatagagg cgcagcttta atatccggcg taaaggcaga gaatttcatt
     8701 ggacaaccaa ctgtcagata ttttccacgc aatggttgtt ccacttcgac aacactgccg
     8761 ctttggcgta aagagggatc aagtgaaatt tctttcatac ttaaaaccgg tgcacaagga
     8821 atatcaaact gagtcaaata agccactgct tcatgtttat caatagtgac agtgtatttt
     8881 tcgatttcag caaaaatatc gaaaatatgt ggctgtcggg catgggctgt actgtatgcc
     8941 ggatcggtaa tccaatctgg tttgccgatg gctttacagg tgttttccca gttttgctcc
     9001 tgaatagtga aataaatata ggcgttagga tcagtttccc agcctttaca tttcaggatc
     9061 cagccaggct gaccgccacc acccgcatta cctccgcggg gaactgcatc accaaatgtg
     9121 ccattcggat actgcgggta ttcttccaga taacccaatt tatcgagacg ctgctggtcg
     9181 cgtaatttca cgcggcaaag gttcaatacg gcatcctgca ttgacatggt gactcgttgc
     9241 ccacgccccg ttttttcgcg atgcagcaaa gcagcaagta aaccgatcag taaatgcatt
     9301 ccggtgttgc tgtcacccaa cgctgcagcg cttaccagcg gcggaccgtc ccaaaaaccc
     9361 gtagtggatg ccgcgccacc cgctgcctga gcaacgtttt cataggcttt tacattcaca
     9421 taaggcgaac actcatcaaa ccctttgatc gaaccaaaaa tcagacgtgg attgatttct
     9481 tgaatatgct cccaggtgaa gcccatgtga tcaatggccc ctggatgaaa gttctcgact
     9541 aagatatcag cttcgcggat cagcttttcc attacctctt tgccttccgc tgttttggta
     9601 tttaactcaa tagaacgttt gttactgtta agcatggtga agtaaagcgc atcgatatca
     9661 ggaatatctc gcagctggtg acgcgttacg tcaccaacgc cggggcgttc aattttaatg
     9721 acgtcagcgc caaaccaggc cagcatttga gtacaagatg ggccagattg cacaccggtg
     9781 aaatcgagaa ctttaattcc ttgaagtgga gttgacatag caatacctat tagttaatta
     9841 aatatgtgtt aatttagcgt ttatttaagg cataacgccc tctataaata atccgcaaaa
     9901 tatcgtcata gcggcactcg atatttttaa attcctgtat caagtgctga aaatcatttc
     9961 atcgctactt ccaattcctt tctgcatggt aagtagtgag gacgaaaaga agaatatgat
    10021 aaatattggt tcgtggtaat cagagagagt cgcgacattt gtgatgttat tcttacaact
    10081 gtaagaagcg ccggtgattt acaagtaagg gta
//
LOCUS       DAZFBR010000144        10067 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFBR010000144 DAZFBR010000000
VERSION     DAZFBR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.144"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(301..978)
                     /gene="bioD"
                     /locus_tag="ACHJZ2_004259"
     CDS             complement(301..978)
                     /gene="bioD"
                     /locus_tag="ACHJZ2_004259"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4570261.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(971..1726)
                     /gene="bioC"
                     /locus_tag="ACHJZ2_004260"
     CDS             complement(971..1726)
                     /gene="bioC"
                     /locus_tag="ACHJZ2_004260"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO4570262.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(1713..2867)
                     /gene="bioF"
                     /locus_tag="ACHJZ2_004261"
     CDS             complement(1713..2867)
                     /gene="bioF"
                     /locus_tag="ACHJZ2_004261"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO4570263.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(2864..3904)
                     /gene="bioB"
                     /locus_tag="ACHJZ2_004262"
     CDS             complement(2864..3904)
                     /gene="bioB"
                     /locus_tag="ACHJZ2_004262"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO4570264.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAIGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3991..5280
                     /gene="bioA"
                     /locus_tag="ACHJZ2_004263"
     CDS             3991..5280
                     /gene="bioA"
                     /locus_tag="ACHJZ2_004263"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO4570265.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQINAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            5339..5815
                     /gene="ybhB"
                     /locus_tag="ACHJZ2_004264"
     CDS             5339..5815
                     /gene="ybhB"
                     /locus_tag="ACHJZ2_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO4570266.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            6133..6225
                     /locus_tag="ACHJZ2_004265"
                     /pseudo
     CDS             6133..6225
                     /locus_tag="ACHJZ2_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6322..7020)
                     /gene="nleD"
                     /locus_tag="ACHJZ2_004266"
     CDS             complement(6322..7020)
                     /gene="nleD"
                     /locus_tag="ACHJZ2_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFO4570267.1"
                     /translation="MRPTSLNLVLHQSSRSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRAESQKYSPLLLEEARTVGLGAFSEEVLSENKFHEEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(7082..7203)
                     /locus_tag="ACHJZ2_004267"
                     /pseudo
     CDS             complement(7082..7203)
                     /locus_tag="ACHJZ2_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(7251..8132)
                     /gene="nleH1-1"
                     /locus_tag="ACHJZ2_004268"
     CDS             complement(7251..8132)
                     /gene="nleH1-1"
                     /locus_tag="ACHJZ2_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HFO4570268.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFVDTTETNVLYDRAKNEFNPIDISSYNVSDRSWSESQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(8301..8456)
                     /locus_tag="ACHJZ2_004269"
                     /pseudo
     CDS             complement(8301..8456)
                     /locus_tag="ACHJZ2_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            8735..8839
                     /locus_tag="ACHJZ2_004270"
                     /pseudo
     CDS             8735..8839
                     /locus_tag="ACHJZ2_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(8959..9978)
                     /gene="nleC"
                     /locus_tag="ACHJZ2_004271"
     CDS             complement(8959..9978)
                     /gene="nleC"
                     /locus_tag="ACHJZ2_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO4570269.1"
                     /translation="MICRVLDINMKIPSLQSNFNFSAPAGYSAPIAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEKVHIGCIKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEA
                     GENDNEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVD
                     KHNQQ"
BASE COUNT         2511 a         2600 c         2316 g         2640 t
ORIGIN      
        1 aattatctaa ccagttaaaa aataaaagat atttctttga gccgtcttta acgccaattc
       61 gcgtgcaagc cgcgtattct ctcgcttgcc tcgtgttttc taactctaat acacatggtt
      121 atccacagga atagtggata actgtctgca gcccctatcc cccgccgcct gggcaattcc
      181 cacattcgcc gcatgatgcg gaagtaaaat ttttttcgtg attattttag aatttaattg
      241 gttaaattgc agtcaatcga agacgcgatc tcgctcgcaa tttaaccaaa tacaggatgg
      301 ctacaacaag gcaaggttta tgtactttcc ggttgccgca ttttctggat tttctgcaag
      361 ccaggggatc tctcccagca gcggcgcggg aatcatgcgg gtgagcgtgg tcatatattc
      421 agcgtgacgt tttcccggag gcgtgacatc gttcgccacc caacccgcca gagtcagccc
      481 ggcgtgttgt atcgcctgtg ccgtcaacat cgcgtgatta atacagccaa gtttcacgcc
      541 aaccaccagt attaccggca gttgttcctg tgttacccaa tccgcaaaag tgaaagtgtc
      601 agaaagcggc gtaaaccagc cgccagcgcc ttccactaac acccagtcag cctgttgttc
      661 aagcgcgcgt aatccggagc tcattaccga tgattctatc gacctgccct cttgcgcgct
      721 gatgatgtgc ggtgaggtgg gttctgcgaa ggtgtaagga tttaccgttg cgtaatccag
      781 ctgcaggctg ctgttgcgct gtaacgccag cgcgtcacta ttacgtaaac cctccggggt
      841 cttttcgctg ccagaggcga ccggtttata acctgccgtc cgatagcctg ctgcctttgc
      901 ggcttgtaaa agtgcacaac tggcgacagt tttccccact tcggtatccg ttccggtgac
      961 aaaataacgt ttactcacga acaatcactc ccaaaaaaag atgatacgtc agaggatatc
     1021 gcccctgttg ttgcggccag gccagttgca atcgctgcaa ctgtgaacgc gttaatattc
     1081 gctggtcgcg cccttcatga agatgcgtgg caccgatgcc tttcagcgaa cgcatggcac
     1141 tgagcgcatc atcaaaccat agtgtgatgg gctgaatacg atgttgataa tgcacgccgt
     1201 tcagcgactg ttcgatttca tctggcggta aaaagcgatt agcatgcggg cgctcgtcca
     1261 ccgcctgcca cgcctgatgc agttcgggta acgatccctg caccagcgtg gtaaacgcga
     1321 ccacgcctcc ggggcgcacc acccgataca gctcgcggag tgccgttgat aaattaccgc
     1381 accactgcac cgcgagattg ctccatgcaa gatcgaacgt cgcagtcgct aacggcaggg
     1441 attcgatatc tcccgccaga taatggtctg cagcatcttt ctggcgggct tgcgcaagca
     1501 ttggcggcga gagatctaac gccgtcacct gcgcgtgacg ctctcgccag tggcggctca
     1561 tccagccagg cccacacccc gcgtccagta cgtgggggta tttacgctgt gggagcattg
     1621 ccagtaagac atcagcactc tggcgctgta actctgcgtg ttgctcatag tgcgcggctg
     1681 cccgaccaaa tgccgctgca atggcttgtt tattaaccgt tgccatgcag cacctccagc
     1741 agacggtcga tatcctgcat ttcatgcgcg gcggttagcg ttaagcgcag tcgcgcagta
     1801 ccagagggta cggttggcgg gcgaatcgcc gtgacccagc agccttgctg gcgcagtttt
     1861 tctgccagtt gtaacgcacg gctgttatca ccgacgatca atggctggat ggcgctgcat
     1921 gaatcagcaa gcgtaaacgg caaatcctgt actccggcac gaaaacgcgt aatgagtgcc
     1981 gccagttttt cgcgccgtgc atcaccctca tcactgcgaa tgaccgccag tgacgcacgt
     2041 aatgcctgcg cctgagcggg cggcatactg gtgctgtaga taagatggcg ggcgaattgc
     2101 agcagataat ccgccaccgt actggagcaa agcaccgcag ccccgctgac gccaaatcct
     2161 ttgccaaaag tcactaccag caattctggt tttacctttt gcaaccagca ggtgccgcgc
     2221 ccctgctccc cgataacgcc cgtgccgtgg gcatcatcga ccatcaacca gccattgtgc
     2281 tgttgcgtta cctgctggat ttccgccagt ggcgcactat cgccgtccat gctgaacacg
     2341 ccttctgtca ccaccagttg ctgccccgga cagggggaag caagcagtcg cgccagatga
     2401 gtgacatcgt tatgaacaaa acggcgaagc tgcgacgggc ttaaactggc agcctccagc
     2461 aatgaggcat ggctaagccg gtcagcgaca atgcggtcct cttttgccat catcgcggca
     2521 atgaccgcct gattagcggc aaaaccagag ataaatagca gtgcccgcga atagcccagc
     2581 cactcggcca gttcttcttc cagcgcctga tgcgcaacgc tataaccgct gacgtgaccg
     2641 gaaccgccgc taccgacgcc aaattgctcc gcaccctgct tccaggcacg gataatttgc
     2701 ggatgatggc ttaaacctaa ataatcgtta ctggaaaagt tcagatactg gcgatcgtcc
     2761 gccaccagcc agcgtccggc tccttgcgcc actggataac ggcgacgcag ggcatcggca
     2821 gcacgccgcg catcgagcgc cgcgttgatt ttctcctgcc agctcatagt gctgccgcgt
     2881 tgtaatattc gtcagtgtcc ggggtcatca gtgcctgctc cagacgctgc tgttgttcgt
     2941 tatcccctgc cagcacggca gtttgctgcg gatttagccc cagtttgcga aacagttgca
     3001 ggtctttatc ttcttccgga ttgggcgtgg ttagcagttt gcaaccgtag aaaatcgagt
     3061 ttgcgcctgc cataaagcac atcgcctgag tctgttcgtt catctgctcg cgtccggcag
     3121 aaaggcgcac gtaagaggtc ggcatcatga tccgcgctac ggcaatggtg cgaataaaat
     3181 caaaggcatc gacatcatcg ttatctgcca gtggagtacc tttcaccttc accagcatgt
     3241 tgattggcac gctttccggc ggcgtcggca ggtttgccag ttgcagtaat aatccggcgc
     3301 gatcttttac cgtttcgcct aaacccacaa tgccacctga gcagactttg atcccggcat
     3361 cgcgcacttt ttccagcgta tcaaggcgtt cctgataagt acgtgtggtg atgatattgc
     3421 cgtaaaactc cggcgaggtg tcgaggttat ggttgtagta atccagcccg gcattcgcaa
     3481 ggcgctgcgc ctgagattca ctcaacgtac ccagcgtcat acacgcctcc agccctatcg
     3541 cttttactcc ctgcaccatt tgttccaggt atgacatatc gcgttcgtgg ggattcttcc
     3601 acgccgcgcc catacagaag cgcgtcgatc ctgccgcttt cgctttgcgc gccgactcca
     3661 gcacctgttc aacttccatc aaccgctcgg cttccagccc ggttttgtag cgcgagcttt
     3721 gcgggcagta tttgcaatct tccggacaag ctccggtctt aatcgacagc aaggtgctga
     3781 cctgcacctg acgaggatcg aaatgctggc gatgcacctg ctgcgcttca aacagcagat
     3841 ccagcaacgg tttttcaaat aattctgtga cttgcgacaa tgtccagcgt gggcggtgag
     3901 ccatggggct tctccaaaac gtgttttttg ttgttaattc ggtgtagact tgtaaaccta
     3961 aatcttttca atttggttta caagtcgatt atgacaacgg acgatcttgc ctttgaccaa
     4021 cgccatatct ggcacccata cacatccatg acctccccgc taccggttta tccggtggcg
     4081 agcgccgaag gttgcgagct aattttgtct gacggcagac gcctggttga cggtatgtcg
     4141 tcctggtggg cggcgatcca cggttacaat cacccgcagc ttaatgcggc gatgaagtcg
     4201 caaattaatg ccatgtcgca tgtgatgttt ggcggtatca cccatgcgcc agccattgag
     4261 ctgtgccgca aactggtggc aatgacgccg caaccgctgg aatgcgtttt tctcgcggac
     4321 tccggttccg tagcggtgga agtggcgatg aaaatggcgt tgcagtactg gcaagccaaa
     4381 ggcgaagcgc gccagcattt tctgaccttc cgcaatggtt atcatggcga tacctttggc
     4441 gcgatgtcgg tgtgcgatcc ggacaactca atgcacagcc tgtggaaagg ctatctgcca
     4501 gaaaacttgt ttgctcccgc cccgcaaagc cgcatggatg gtgaatggga tgagcgcgat
     4561 atggtgggct ttgcccgcct gatggcggcg catcgtcatg aaatcgcagc ggtgatcatt
     4621 gaaccgattg tccagggcgc aggcgggatg cgcatgtacc atccggaatg gttaaaacga
     4681 atccgcaaaa tgtgcgatcg cgaaggtatc ttgctgattg ccgacgagat cgccaccgga
     4741 tttggtcgta ccggcaaact gtttgcctgt gaacatgcag aaatcgcgcc ggacattttg
     4801 tgcctcggta aagccttaac cggcggcaca atgacccttt ccgccacact caccacgcgc
     4861 gaggttgcag aaaccatcag taacggcgaa gccggctgct ttatgcacgg gccaactttt
     4921 atgggcaatc cgctggcctg tgcggctgca aacgccagcc tggcgattct cgaatctggc
     4981 gactggcagc aacaggtggc ggctattgaa gtacagctgc gcgagcaact tgcccccgcc
     5041 cgtgatgccg aaatggttgc cgatgtgcgc gtgctggggg ccattggcgt agtcgaaaca
     5101 actcgtccgg tgaatatggc ggcattgcaa aaattctttg tcgaacaggg tgtctggatc
     5161 cggccttttg gcaaactgat ttacctgatg ccgccctata ttattctccc gcaacagttg
     5221 cagcgtctga ccgcagcggt taaccgcgcg gtacaggatg aaacattttt ttgccaataa
     5281 cgggaagtca gcgtgagagt ttctggctac actttctgca aactagaaag gagggtttat
     5341 gaaactcatc agtaacgatc tgcgcgatgg cgataagttg ccgcatcgtc atgtctttaa
     5401 cggcatgggt tacgatggcg ataatatttc accgcatctg gcgtgggatg atgttcctgc
     5461 gggaacgaaa agttttgttg tcacctgcta cgacccggat gcgccaaccg gctccggctg
     5521 gtggcactgg gtagttgtta atttacccgc tgatacccgc gtattaccgc aagggtttgg
     5581 ctctggtctg gtagcaatgc cagacggcgt tttgcagacg cgtaccgatt ttggtaaaac
     5641 cggatacgat ggcgcagcgc cgccgaaagg cgaaacccat cgctacattt ttaccgttca
     5701 cgcgctggat gtagagcgta ttgatgtcga tgaaggagcc agcggcgcga tggtcgggtt
     5761 taacgttcat ttccactctc tggcaagcgc atcgattacc gcgatgttta gttaatcact
     5821 ctgccagatg gcgcaatgct atctggtatc acttaaaggt attaaaaaca actttttgtc
     5881 tttttacctt cccgtttcgc tcaagttagt ataaaaaagc tgaacgcgaa acagcaaaac
     5941 ccaataatat caatgtatta caatgcattt agtctaaaaa atagactgca taatgctaca
     6001 aaacacaaca tatccagtca ctatgaatca actacttaga tggtattagt gacctgtaac
     6061 agagcattag cgcaaggtga tttttgtctt cttgcgctaa ttttttgtca aacacattac
     6121 gtcgtttgct gaatttacaa ccatgctcag tatctctata agcgcagaga aatgatgcag
     6181 tggtgggtgg gctggcttga tgaaaaggtg gggtactcca cctgaaccac tatcggaata
     6241 gcacaaagcc ttgcaatcca gtgcaaagtt ttgtgtgcct ccgtttgccc caccaatata
     6301 tattcagcac aagaaacaca gctaaagcaa tggatgcagt cttacctgtt ggaatcccag
     6361 actcactgta ttgtcatcat gaataagagc tgagtcgtgc gggtaggagg ttctacgggg
     6421 catcccaatc tcttcgtgga atttattttc tgaaagcacc tcctctgaaa aagcccccaa
     6481 cccaacagtc ctggcttctt cgagtaaaag tggagagtat ttttgtgatt ctgctcggga
     6541 actctcaact ttcaaacgct ccccatttaa attgtggaaa acatggagca actcatgaaa
     6601 aacaatagtc gcatgaaagt ccaccacgct aatcccctcc ccacagggat attcaactgc
     6661 attcagatta cagtgaaaat cggaaccagt cccccgatgg ttctcagcat ctatatctct
     6721 atgtgccata actcctagtc tggaagagtt gaggtgtatt accactgttt ctgatttaag
     6781 tcgggatatt gattcaatag cattcaggag tacacgccct gtctctgtag agccaatctt
     6841 atcgagtgct gcttcaatct gctgatatat attatgatca gtcccctgga aacgaagttg
     6901 cggattattt tttatccatt gaacgctcga tgcttttaca agagactcga tatctgtatc
     6961 tgacattgag ctcgaccttg atgactgatg taataccaag ttgagggacg tagggcgcat
     7021 caggactcct gagaatgcta cttattagca gcctgtttac cactctgtga aaatgatttt
     7081 tttaaaagtg accgtcaagg cggtcaccaa acccgataat aaagcggatc atagccattt
     7141 gccagtcctc agcgacattg cccatttccg tgatccgcca agaattgcca accacacaat
     7201 ttgttaccaa ctccatccat gcaacaaatc cactacactg gataaaatta ctaaatttta
     7261 cttaatacca cactaataag atcttgcttt ccgccatgat aagattgcat tatttgactt
     7321 tcactccatg aacggtcgga aacattataa gatgatatat ctattggatt aaactcattc
     7381 ttcgcgcggt catataagac atttgtctct gttgtatcga caaaaagaat tcctttttgc
     7441 tccagtctat caaacatatc gtaaatagca tgctcagcct gtgctggcaa ggacgaaata
     7501 tttaaaagcg attctccatt tattttatcc attctaatac caataatatc accattattg
     7561 ccatatattt tttctgcact cccggcacca taatattggt tgaagcaacg aacttcgctt
     7621 gtcacctctt cattgctttg agatgtagta aacatcttca ggacttttgt tgcatcctca
     7681 gcatcttcat atactacagc gttcccccct ttgccaataa cattaccagg tactgacaac
     7741 ctgttatagt ccacactcgg taactctggc ggtgcataat caacaggagg taaatcaggt
     7801 ctgttcgaat gaacaatgcc tctttccata gcagcgctca ctggtgatgc attcagcatg
     7861 acctcaattt tcctgctaat ctccccctta ggccatccca gcctgtgcaa cagattagta
     7921 aaacaaccac tatgactttc tcttgtaacg caaaacttat tatcggtggc aacaacacga
     7981 tatgttctgt tgccaacctt tacttgcgcc ccattatcag aatgaacggc agcatccctt
     8041 acagaggata aaacacgatt atcaggcgaa gtcaggtttc tggttaaaga attccatgaa
     8101 catcccaaat ttacagaata tggcgataac atacatttca accttcaaaa taaacccatc
     8161 taaatttcct taacaaacat ccccctaaca tggcaacaaa aattagaaat gaactcttgc
     8221 tttttgtatc tggcaactaa ttcatccttc cagcatccca tcaaacgaac gtttacgaac
     8281 tataacgcag gtattaccgg tcactcgata tctggagata cagacgtatc aacatgattg
     8341 agcggcatac gatactgctt ccatgccgcc agtaacgatg tctgctcatc cgtcgcaata
     8401 tctaaatctg cagcatcctg aagtggtgcg atatactcac tgattacaag taccagaccg
     8461 atagattaaa aaaaacacaa taacacgctg catttaaaac aaaaaatttt aaatatgatt
     8521 taatacaagc tttaagcctt taattatcag ttttatttat tttccagcaa ttgattaatc
     8581 aattgtttat atacaaatta ttccttttct ttctctcacg caaaattcac aaaatattgt
     8641 tgctgcaatg gacctgtaca atagtgaatc acggtacttt tctgtgcaca acgtactgca
     8701 gtttctttac gatttatagc tgtactgttg aattatgtac aatttgaatc catgcatgac
     8761 gtgtgatgcc tattgtgcat ttttccgagc ctctttttac tgggccgaag ccgatgatgc
     8821 tgatagtctc gttccggcca tgttcactta ctacagtaaa attaggggaa ttgcctttac
     8881 atacaatgtt gcacgattac ccatcatcaa ctccgttaaa atgtatgaat agtaacctta
     8941 tgtcactgca aagacgaatc attgctgatt gtgtttgtcc acatccccaa atgtaaaaat
     9001 ataaggggcg ccatgataca gttggatctg atttgcatcc tgtaaattat cgttgatagc
     9061 caatccagga aaatcatgtt gctggataaa tccgtatcct atgttagaca cagcggaata
     9121 ctctgtgaaa tcaggactcg cctcatatcg gtcactgatc gtacccagcc tttcgaagaa
     9181 agccctctca ttctcttcat gcctcatggc agcctgtcga agggcattca ggttacgtag
     9241 cctaagatga gcttcacgtt ctggctctgc atatcctttg aagtcgggaa cactccatcc
     9301 cagttcttga gcgacacgac gtgcgagaat ctcggtgggt cctaactcta tattactatc
     9361 tccagatgga tcgctagatc cagtaacatg atgaataatc tcgtgaatca gtccttcctg
     9421 ccacgatggc atctcataag agtcagtatc tggcgcaaca ctaaaactga cataaggctc
     9481 ttcgttatcg ttttctcctg cttcgcaaat ggggaaaatt ggctcttgcc cgacatcata
     9541 ttcatataat tcgttacagg ttagtgattg aatatcatca attttgacag aagattcttc
     9601 gttaagctcg tattcgtttc tgtatttaat gcaaccaatg tgtaccttct cattatgaat
     9661 gccatagctt acggcatcac gaaatgtctg tgatcggctc aaagcatcaa gtacagtgtt
     9721 gccgatcata tcgactgtat gctgatcgat aagcctgcta cggctgtcat ggacggcgcg
     9781 tattacactt tcgtatacgt tgcttaaatc tgctgcggaa agtggtattc gcttacctat
     9841 atccaaaacg taatccgcat aggcattttc agcacgatta ggagcaatgg gagcagagta
     9901 tcctgccggg gcggaaaagt tgaagttgga ctgtaatgag ggaattttca tgtttatatc
     9961 taataccctg caaatcattt attacaacaa aataaactat atcaaattca tttaccatga
    10021 actgcatgta tactgacttg tgttcatgat tatattaggg tggttaa
//
LOCUS       DAZFBR010000145         9941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFBR010000145 DAZFBR010000000
VERSION     DAZFBR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.145"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(41..916)
                     /locus_tag="ACHJZ2_004272"
     CDS             complement(41..916)
                     /locus_tag="ACHJZ2_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO4570270.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            complement(977..1879)
                     /gene="yihR"
                     /locus_tag="ACHJZ2_004273"
     CDS             complement(977..1879)
                     /gene="yihR"
                     /locus_tag="ACHJZ2_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO4570271.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            complement(1946..3187)
                     /gene="yihS"
                     /locus_tag="ACHJZ2_004274"
     CDS             complement(1946..3187)
                     /gene="yihS"
                     /locus_tag="ACHJZ2_004274"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO4570272.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            complement(3203..4081)
                     /gene="yihT"
                     /locus_tag="ACHJZ2_004275"
     CDS             complement(3203..4081)
                     /gene="yihT"
                     /locus_tag="ACHJZ2_004275"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO4570273.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(4105..5001)
                     /gene="yihU"
                     /locus_tag="ACHJZ2_004276"
     CDS             complement(4105..5001)
                     /gene="yihU"
                     /locus_tag="ACHJZ2_004276"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO4570274.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5169..6065
                     /gene="yihV"
                     /locus_tag="ACHJZ2_004277"
     CDS             5169..6065
                     /gene="yihV"
                     /locus_tag="ACHJZ2_004277"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO4570275.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            6099..6884
                     /gene="csqR"
                     /locus_tag="ACHJZ2_004278"
     CDS             6099..6884
                     /gene="csqR"
                     /locus_tag="ACHJZ2_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4570276.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVAKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6983..7582
                     /gene="yihX"
                     /locus_tag="ACHJZ2_004279"
     CDS             6983..7582
                     /gene="yihX"
                     /locus_tag="ACHJZ2_004279"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO4570277.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            7576..8448
                     /gene="yihY"
                     /locus_tag="ACHJZ2_004280"
     CDS             7576..8448
                     /gene="yihY"
                     /locus_tag="ACHJZ2_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO4570278.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            8445..8882
                     /gene="dtd"
                     /locus_tag="ACHJZ2_004281"
     CDS             8445..8882
                     /gene="dtd"
                     /locus_tag="ACHJZ2_004281"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO4570279.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8927..9868
                     /gene="fabY"
                     /locus_tag="ACHJZ2_004282"
                     /gene_synonym="yiiD"
     CDS             8927..9868
                     /gene="fabY"
                     /locus_tag="ACHJZ2_004282"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO4570280.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2375 a         2498 c         2651 g         2417 t
ORIGIN      
        1 aagaatgtgt gcggatataa atttaaaccc gcataagtga ttatttactt gcttcagcct
       61 gacggattgc cgccactttt ttaccaaata ccagttgcag tataactaac aaggtaaacg
      121 gaggtagaag tgcggcagcg gcgggaattc ccagcgcgcc ttttatcaac atggaaccgg
      181 aggcaatcag caataaaaag accatcacac ggaacacttt gactgaaata cgtttatgca
      241 catattgtcc aaccagtgaa ccgaggatca tggtgggaat acaaaacagc accagtttaa
      301 agatcgaaac agtcaaaatt ccgctggtcg ccagcccgcc gatcaccgcg atgttgtttg
      361 cggtgaagaa agcgttcaga gtgccacgaa atgccgccgg gcctaagtta cgcagcatac
      421 cgtagataac catcggtggt ccgttggttg agaaagctgc gcctaatgcg cctgctactg
      481 caccgatagg tgcggcgatc cagctacggt cataaatcgg caggcgtgga ataaacatac
      541 tgtaaaagga gtacaggata aggaatccgc ctaagcccac tttcataaaa tggtcgggta
      601 aatacgacag tgcatataaa ccgacgggta ttcccagaaa cgagaaaata atcagtacca
      661 ttgccgatcg ccaatccgtt tctttacgtg acagccaggt ggcgtacaag gcggtcgcgg
      721 tgccgacaat taccgagagt ggtgttgcca tttttacggg gagcattaac gtgacgagcg
      781 gcatagtggt taaaccaccg ccaaatcccg cgcatatccc cacaaaggta taaagaaaca
      841 ttaacgcgat aacgatacat accacgtttg tttccagcat gaccccatcc ggtgaaaata
      901 acgtaaaaaa gtccataata tttcctgttg tgtaaataca actctaaaca atgatctcgt
      961 tcttttctta ataaaattaa ttgtgttgcc cgtaaatatt aaaaaacagg cggtgcgttt
     1021 tccctggttc cagtaataat aagtcgagac ctgaattaaa ggcgttaggc gggcaactca
     1081 taggttcaac tgccagtccc tgacgctgta atttttcacc gctataaatt tgtacccagg
     1141 gttgatcgga acaaaggcta accgaaagcg cctgttgtgg atgggtaatg gttatttccc
     1201 acagatcatt agcggtttta aaggtgtggt cgatttttgt cgcggctatt tttttcgcct
     1261 gcgtgaaatt taaatccagc tcatcaacgt ggtgaagcgt tgtcggatta gcgtgctcat
     1321 cgacggcata aacctggttt gccggaagct ggaacagata ttcatcaaca gatgttaagt
     1381 tgcaggttaa ataaggatga atacccacac catagggtgc ggtcacagta ccaatattct
     1441 gacttgcgat ctcaacggaa agacccgtgc gtgcattgag cgaataaaca acctgcgaag
     1501 ccagcataaa cggatagccg taactgggcg gcagaaaagc tgtcagggta acgctggtgg
     1561 cggttaattc gctgatttgc cagtcacgcc aggcgagcag cccatgaata gcggcttttg
     1621 aaccatgttc attaatgggt aactgatatt cctgcccctg atagcgataa cagccgttgg
     1681 cgatccggtt cggccacgga ataaggactt tgcccagatg tgccagcggc atctcttcgg
     1741 gtttatgtgg aataactaaa tgacagccct gaaaggttaa ttcagccaga cctgcaccga
     1801 cggtcacaat ggtcgcgtga taatcccccg cggcaagtga aacggtctgt ccactgcaat
     1861 gcatgttggt gatttgcata cgaggtacct ttattgaata acgacatacc cggcgtgcag
     1921 cctgatatgt cgttttttta gtcagttatt tcgcgttaat atccagcaga cccgccgcaa
     1981 ctgccggagc caggcctggc gctaacggga tacgcgggat caccaggcaa tgcagcaggt
     2041 gataaatatc ctgtttgccg tcccagactt tggtggtgac cttattgtcc gcatccagct
     2101 cctgccacca tgaaccattt tcgtagtcca tcaggtattt gatgcagtag tcccaccatg
     2161 tctgatacca ggtttcgtac tggcgatcgc cggtgacggt gtagagcgcg taggccgtac
     2221 ccatcgcttc gacgataggc caacgcacac gttcgcggac caccggtttt ccttcccagt
     2281 caacggtata aacaatcccg tccgcaccat cgggtgccca ggcatcacgc acggtggcgt
     2341 taaacagacc tttggcatct tctagcagcc atgctggcgg ttgttcgcaa cgggcttcca
     2401 gggccgcgtg gatgtgcagc attaaacggc cccattcgat ccagtggcct ggtgtacctc
     2461 cgaacgcgcg gaagcggtgc gccgggttat ctttgttgta atccggcagc ggattccact
     2521 gggtatcgaa gtgttcgtta acgcgataat gattatttct tgcgacgtcg tggataatca
     2581 cggaagccac gcgaatcgcg cgatccagcc attttttgtc atgcgttacg tcataaacaa
     2641 ttaagaaagc ttccactgcg tgcatattgg cattgccgcc gcggtactct tcggttttgc
     2701 tgaaggcttc gtcccaggac tccaggcaca tctgctcttc ttcgctccag aagtatttct
     2761 cgataatttc aatggtgtaa tcgagcagct tgcgcgcttc cgggtgaccc gttgtgacgg
     2821 cgctggcagc acccagcaga gcaaagaaat gttgatagcc ctgtttggag gcatccacca
     2881 cgccctcgtc attcacacag gcataccagc cgccatattt tttatcgcgc agtgcgccgt
     2941 tcatggcttt aataccgtga tcaaccaacg agtaagcgcc aggtcgaccc atcgccgcag
     3001 caacggaata aacgtgcaac atacgagctg tgatccacag atgggtgccc atctcttctt
     3061 tgatttgccc tttattgcct aaccagccaa atccggtcgg tactacggaa tttttaccaa
     3121 aatcaaagat gcgatcggtt tcctgttcca gccaacggtt gtgacttaaa gtgttaaacc
     3181 atttcatttt tgtatcccca ttttaacggc gtttagccat catttcgtcg acgatatcgc
     3241 caagttgttg taatttcggt gcgcaaacgt cacgcagcat cagctcgttg tctggtaaac
     3301 cgacgaccga tgcccagaca gcacgaccag ccaggaagcc cgatgcaccc gctgtcattg
     3361 ccacgcgtac ggcacgcggg aacagttttt cgtcgacacc ggaagagagg atcacccacg
     3421 gcatattgat atggtcattc agacgttgtg aagcactgag aagctcttgt tgcggacctt
     3481 taccgtaaag cggcatttca actttgtaga ggtcagcgcc actgtcgccc agctctttgg
     3541 cggcatcgat gatcgcttgt tcgcgatcga atttatcgcc acgacgtggt gggcggacga
     3601 ctggctcaat aatgcttacc aggccgtgtg agtggcacag ttcgttaaac tctttcacca
     3661 tatccaggcg ttgctgcgca tcttcatcac tacgccacag caccagcagt tttaaggctt
     3721 tgccaccgtc ttgtttgatc tgtagcggat tgattttgcg gtcaatgacc acgctatcta
     3781 ccggaatacc gttgccagga atgaactcat cggcggcgac aatcatggcg caacttttgg
     3841 caatcgcgtt ttgctcaacc acctggcggt aacagaattg ttgatctacc agaatcgccg
     3901 aggcataagg cgagagggtc ttcgccgcgt taactttgaa atcagttaaa acgctatcgg
     3961 ctaccggagc gggtgcccca gccgcggcaa acatcatgcg catggcttcg cgctgatcga
     4021 ccgccagcat ggcaaaaccg cccgatgcgc gcgtaatgtc gttgatggtg tacttattca
     4081 ttcatttatt cctttatcca gtcgttacat ttttactttg gcagtcatcc cggcactgac
     4141 acggacctgt tccagaatgg cggaccagtc ctggcgaccg cgacccgctg cgcgcgcctg
     4201 gctataaacc tcccgtgagg cggcccccag cggcattggc acatgcagct ggttggcgac
     4261 atcaagggcg atgccaagat ccttatgggc aagatcgatc atgaaggcgg gagaaagatc
     4321 cccgctgagg actttgttcg gccaggaagt ggtgaagtgg cctttaccgg cggcggtacc
     4381 gctcatcact ttgacggcaa catcgaaggg aagattcagg gcttcgcaca aaacggcagc
     4441 ttctgccgaa agcgcattga gcgcgatgct catatagttg ttgatgagct taacgcggat
     4501 ccccatgccc ggaccgcctg cgttgatcaa ctcactgccc atcgccatca ggatcggcgt
     4561 ggcacgttca acttgttcag cggtgccgcc agccagcagt aacagagtac cggtaatggc
     4621 atttgcagaa gtacggccta ccggaacatc catcatgttg aagcctttgg cttgcatatc
     4681 ggcaatcaat ttatcggttt gcagcggatg gatggtcgac atatcaatga ccagcgcatc
     4741 ggtagataag ctttcgcaaa cgccgttttc accgaacaac acgctgcgca ccagatcgcc
     4801 attcggcaac atggtaatga taaattcggc atctttagcg gcctgcgccg ggttggcggc
     4861 gggagtcgcg cctttgtcta ccagatgccg cacagcctcg gcattcacat caaagacgcg
     4921 aagttggtgc ccttgctgca ataaattgct cgccattggc gaacccattt gtcctaaacc
     4981 gataaacgcg attgctgcca taaccttctc ctgaatacag tgatgtcact ttttgtcatt
     5041 tatgacacgc tttgattgtc tatttttgat cgtatttgta atttatagtc aaaaaattga
     5101 cagccgtcac tttttaaaca attggtgaaa ttaaaatgaa cgcatcccaa aatgtttaag
     5161 gaatgaccat gattcgtgtt gcttgtgtag gtataaccgt gatggatcgc atctattacg
     5221 tggaagggtt accgacggag agcggtaaat acgtggcgag aaattatacg gaagttggtg
     5281 gcgggccagc ggcgacggca gcggttgcgg cggcaagact gggagcgcag gttgatttta
     5341 ttgggcgcgt cggtgatgac gacaccggca acagcctgct ggcagagctg gaatcctggg
     5401 gcgttaacac ccgctacacc aaacggtata aacaggcgaa atcgtcgcaa tccgccatca
     5461 tggtggatgc caaaggcgag cggataatca ttaactaccc cagcccggac ctgctgcctg
     5521 acgcagagtg gttggaggaa attgatttct ctcagtggga tgttgtgctg gcagatgtac
     5581 gctggcacga cggcgctaaa aaagccttca ctctggcccg gcaggcgggc gtgatgaccg
     5641 ttctggacgg tgatattacg ccgcaggata tcagcgagct ggtggcatta agcgatcatg
     5701 cggccttttc agaaccgggt ctggcgcgct taacgggcgt gaaagagatg gccagtgcgc
     5761 taaaacaggc acaaacgctc acaaatggtc atgtctatgt gacccagggc agcgcaggct
     5821 gtgactggct ggaaaatggc gggcgtcaac atcaaccggc cttcaaagtt gatgtggtag
     5881 ataccacagg tgcgggtgat gtttttcacg gcgcattggc ggtggtgctg gcaacaggtg
     5941 gggatttagc ggagtcagtc cgcttcgcca gcggtgtagc ggcgtcgaaa tgcacgcgtc
     6001 ccggtggacg agccgggatc cctgactgtg atcaaacccg atcttttttg tcactttttg
     6061 tataaaatgc cagggtgatg gtttttcgag gaattttcat gagccttacc gaactaaccg
     6121 gtaacccgcg gcacgaccaa ctcctcatgc tgatcgccga gcgtgggtat atgaatattg
     6181 atgagctggc aaatctgctg gatgtctcca cgcagacggt gcgccgggat attcgtaaat
     6241 taagcgagca aggcctgatt acgcgccatc acggtggcgc gggtcgggct tccagcgtcg
     6301 ttaatacggc gttcgagcag cgtgaggttt cgcaaaccga ggaaaaaaaa gcgattgccg
     6361 aagcggtggc agactatatt cctgatggtt caacaatatt tatcaccatt ggtacgaccg
     6421 ttgagcatgt tgcccgggcg ttacttaacc ataatcattt gcggataatc accaacagcc
     6481 tgcgtgtggc gcatattctt taccacaacc cgcgctttga agtgatggta cccggcggta
     6541 cgttgcgctc tcataatagc gggatcattg gcccttcagc ggcgtccttt gtggccgatt
     6601 ttcgtgctga ttatctggta acaagcgttg gggcgattga gagcgatggc gcgttgatgg
     6661 agtttgatgt aaacgaagct aacgtggcga aaacgatgat ggcgcacgcg agaaatatcc
     6721 tgctggttgc cgatcacact aagtatcatg cttcggcggc ggttgaaatt ggtaacgtgg
     6781 cacaggtcac cgcgctcttt accgacgagc tgccgcccgc tgcgctaaaa tcacgcttac
     6841 aagacagcca aattgaaatc atccttcccc aggaagacgc gtagattttt gtgactgttg
     6901 acctggcttc ataccttgcc acatagccaa acccatcctt tcccgctaca gttaatttct
     6961 tgtggcgcga aaggaggcaa aaatgctcta tatctttgat ttaggtaatg tgattgtcga
     7021 tatcgacttt aaccgtgtgc tgggaacctg gagcgattta acgcgtattc cgctggctac
     7081 gcttaagaag agttttcata tgggggaggc gtttcatcag catgagcgtg gggaaattag
     7141 cgacgaagcg ttcgcagagg cgctgtgtca tgagatggct ctaccgctaa gctacgagca
     7201 gttctctcac ggctggcagg cggtgtttgt tgcgctgcgc ccggaagtga tcgccatcat
     7261 gcataaactg cgtgagcagg ggcatcgcgt ggtggtgctt tccaatacta accgcctgca
     7321 taccaccttc tggccggaag aatacccgga aattcgtgat gctgctgacc atatctatct
     7381 gtcgcaagat ctggggatgc gcaaacctga agctcgaatt taccagcatg ttttgcaggc
     7441 ggaaggtttt tcacccagcg atacggtctt tttcgacgat aacgccgata atatagaagg
     7501 agctaatcag ctgggcatta ccagtattct ggtgaaagat aaaaccacca tcccggacta
     7561 tttcgcgaag gtgttatgct aaaaaccatt caggacaaag ccaggcaccg tacccgtcca
     7621 ctatgggcct ggctaaaact actctggcaa cgcattgatg aggacaatat gacaaccctg
     7681 gcaggtaacc ttgcctatgt gtcgttgctc tcattagtgc cgctggttgc cgttgttttt
     7741 gcgcttttcg ccgcttttcc catgttttcc gacgtcagca ttcagttacg tcactttatt
     7801 tttgccaact ttctgcctgc tactggcgat gttattcagc ggtatatcga acaatttgtt
     7861 gccaattcca acaagatgac cgccgttggg gcgtgcgggc tgatcgtcac cgcgttattg
     7921 ttgatgtact ccatcgatag cgcgttgaat accatctggc gcagtaaacg agcgcgaccc
     7981 aaaatttact cgtttgccgt gtactggatg attttaacgc tggggccgct gctggcaggg
     8041 gccagtctgg cgatcagttc ctatttgctc tccctgcgct gggcgagcga tctcaatact
     8101 gtcatcgaca acgtgctgcg tatttttccg ttgctgttgt cgtggatctc cttctggttg
     8161 ctgtacagca ttgttcctac catccgcgta cctaaccgcg acgcgattgt cggcgcgttt
     8221 gttgctgcac tcctgttcga agcaggaaag aaaggtttcg cgctttatat caccatgttc
     8281 ccgtcatatc agctcattta cggtgtgctg gcggtgatcc ccattctctt tgtttgggtc
     8341 tactggacat ggtgtatcgt cttgcttggc gcggaaatta ctgtcactct cggggaatac
     8401 cgcaaactaa aacaagcagc tgaacaagaa gaagacgacg aaccatgatt gcattaattc
     8461 aacgcgtaac ccgtgccagc gtcaccgtgg agggagaagt gacgggcgaa attggtgcgg
     8521 gacttttggt gttattgggt gtcgaaaagg atgacgacga acagaaagca aaccgtctgt
     8581 gcgagcgtgt gctcggctac cgcatcttta gcgatgccga aggcaagatg aatctcaacg
     8641 tgcaacaggc gggcggcagt gtgctggtgg tttcccagtt taccctcgcc gcagataccg
     8701 aacgggggat gcgcccaagt ttctccaaag gtgcatcacc ggatcgcgca gaggcgttat
     8761 atgactattt cgtcgaacgc tgccgtcagc aagagatgaa cacgcaaaca ggacgcttcg
     8821 ctgcggatat gcaggtatcg ctggtcaatg atggccccgt gacattctgg ttgcaggtat
     8881 gagccagctt ccagggttgt cacgggaaac aagagagagt atcgctatgt atcaccttcg
     8941 ggttccacaa acagaagaag aattagagcg ttactatcag tttcgctggg aaatgttgcg
     9001 taagcccctg catcaaccaa aaggttcgga acgcgacgcg tgggatgcga tggcgcatca
     9061 ccagatggtc gtcgacgagc agggtaatct ggtggcggta ggccgactgt atattaatgc
     9121 cgacaatgaa gcgtccattc gctttatggc cgttcatccc gacgtgcagg acaaagggtt
     9181 aggcacgctg atggcgatga ccctggagtc ggtggcgcgt caggaaggcg ttaagcgcgt
     9241 gacctgtagc gcccgtgaag acgcggtgga gtttttcgcc aagctggggt ttgttaatca
     9301 gggagaaatc accacgccaa ccaccacgcc gattcgccat tttttgatga ttaagcccgt
     9361 cgccactctg gatgatattt tgcatcgcgg cgactggtgc gcgcagctgc aacaggcgtg
     9421 gtacgaacat atcccgctta gtgaaaaaat gggcgtgcgc attcagcaat ataccgggca
     9481 aaaatttatc actaccatgc cagaaaccgg caatcagaat ccgcaccata cgctgtttgc
     9541 cgggagttta ttctcactgg cgacgctcac cggttggggg cttatctggc tgatgctgcg
     9601 cgaacgccac ctcggcggaa cgattattct ggcggatgcg catatccgct acagcaaacc
     9661 gattagcggt aaaccccatg cggtagccga ccttggtgcc ttaagcggcg atctcgaccg
     9721 tctggcgcgc ggacgaaaag cacgggtgca gatgcaggtc gaaatctttg gcgacgagac
     9781 gccgggtgca gtgtttgaag gcacgtatat cgttctgccc gcgaagccat ttggcccgta
     9841 tgaagagggc gggaacgaag aagagtagct gatggagagg ggcggtgctg cctctctcat
     9901 tcaggtcatt tatattagtc cttggctatt tcggttatat t
//
LOCUS       DAZFBR010000146         9931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFBR010000146 DAZFBR010000000
VERSION     DAZFBR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.146"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(262..1020)
                     /gene="yhdZ"
                     /locus_tag="ACHJZ2_004283"
     CDS             complement(262..1020)
                     /gene="yhdZ"
                     /locus_tag="ACHJZ2_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO4570281.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1028..2131)
                     /gene="yhdY"
                     /locus_tag="ACHJZ2_004284"
     CDS             complement(1028..2131)
                     /gene="yhdY"
                     /locus_tag="ACHJZ2_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4570282.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2141..3322)
                     /gene="yhdX"
                     /locus_tag="ACHJZ2_004285"
     CDS             complement(2141..3322)
                     /gene="yhdX"
                     /locus_tag="ACHJZ2_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4570283.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3390..4415)
                     /locus_tag="ACHJZ2_004286"
     CDS             complement(3390..4415)
                     /locus_tag="ACHJZ2_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4570284.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4846..5067)
                     /locus_tag="ACHJZ2_004287"
     CDS             complement(4846..5067)
                     /locus_tag="ACHJZ2_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570285.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5320..8469)
                     /gene="acrF"
                     /locus_tag="ACHJZ2_004288"
                     /pseudo
     CDS             complement(5320..8469)
                     /gene="acrF"
                     /locus_tag="ACHJZ2_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8481..9638)
                     /gene="acrE"
                     /locus_tag="ACHJZ2_004289"
     CDS             complement(8481..9638)
                     /gene="acrE"
                     /locus_tag="ACHJZ2_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO4570286.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2661 a         2547 c         2375 g         2348 t
ORIGIN      
        1 ctccccatgc gagagtaggg aactgccaga catcaaataa aacaaaaggc tcagtcggaa
       61 gactgggcct tttgttttat ctgttgtttg tcggtgaaca ctctcccaac caggacaaat
      121 ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc
      181 ccgccataaa ctgccagaca tcaaatcaag cgaaaggcca tccgaaagga tggcctttat
      241 gcttttcgaa ctaacattca attaatggat tacctgcgat aaaaatgccc tcgtacgctc
      301 tgacttagga tgcgcaaaaa actcatccgg tgcagcttgc tccactattt cgccacgatc
      361 cataaaaatc acccggtcag cgacggttcg cgcaaacccc atctcatggg ttacacacaa
      421 cattgtcata cccgattgcg ccagcccaat catcgtatcc agcacctctt tcaccatctc
      481 cggatcgagc gccgatgtgg gctcatcaaa caacataatt ttcggcttca tacacagcga
      541 gcgcgcaatg gcaacgcgtt gctgctgacc acctgaaatc tgtccgggaa acttatgcgc
      601 atgttcggca attctcaccc gctcaaggta atgcatcgcc agagcttcag cctctttctt
      661 tggcatcttg cgtacccaaa tcggtgccag ggtacagttc tgtagaacgg tcagatgagg
      721 gaagagattg aaatgctgaa agaccattcc cacttcctgc ctgacgcgct caatattgcg
      781 gatatcttca ttaagttcga tgccatctac cacgatccgt ccctgttgat gttcttccag
      841 atgattaata caacgaatgg ttgtcgattt accggaacct gaagggccac acagaacgat
      901 ccgttctccc ggttgcacgg ttaaatttat atttttcaaa acatggaatt gtccatacca
      961 tttattgacg ttttccagcg taatcatcgc gttagcaggt tgcagtaaaa tttggctcat
     1021 agtgtcctca atgcggtgta cgcccggtgt taaaacgttt ttccagatgc tggctatagc
     1081 gcgacatgct gaaacagaag atccagtaga tcagcgcggc gaaaacatac ccttccgtcg
     1141 acatacccaa ccaggcggga tcaacggttg cttgctgaac gctactgaaa agatcgaaca
     1201 acccgataat gatcaccagg ctggtatctt tgaagagtgc gatgatggta tttaccagcc
     1261 caggaattac cagcttcaac gcctgtggca gaataaccag cccctgagtt ttccagtaac
     1321 ccaacgccag cgactctgcc gcttcatatt gccctttagg cagcgcctgt aatccacctc
     1381 gcacgacttc cgcaacatat gctgactgaa acaggatcac gccaaccagc gcgcggatca
     1441 gtttgtcgat actggtgcct tctgccataa acaaaggcag catgaccgaa gacataaaca
     1501 gaacggtaat cagcggcacg ccgcgccaga attcgataaa aatgaccgat aagatgcgca
     1561 caatcggcat atgggagcgg cgacctaacg ccagtaagat cccccacggt agcgcccccg
     1621 caatcccaac tgatgcaata attaaagtta gcgtcagccc gccccattgc cgggtttcaa
     1681 cccgctcaag agcaaaaaaa ccgccataca tcagccacca gacaatcagt gggtaaatca
     1741 ctgcccagac cgcaatatag cgaccgcgat gcgggagtat tttccagaac attggtgcga
     1801 ttgacacaag gccaatcagt aatgccaagt taattcgcca gcgttggtcg tgtgggtaaa
     1861 gtccatacat aaactgacca aatcgttcgt ggatgaagac ccaacaggca ccggcttttg
     1921 tgcagtcggc acgcgttgag ccaacccagt tagcctgcaa aaatgcccag ttcagcaacg
     1981 gtgggatcag ttcccacatc aaccatatgc agccaatagt cagcaggcta ttgctccagc
     2041 tggagaacag attttttcgc acccacacca tcgcgcggct tgagttatgg ctcgccgggc
     2101 gcgagggatg agacagcaat acttttgtca tcatgactcc ttagcgttcc acgatcgcga
     2161 tgcggcggtt atagatattc atcagcagcg agatagtcag gctgataatc agatagaccg
     2221 acatggtcat ggctatcgtc tcgatggctt gccccgtctg gttcagcacg gtgccagcaa
     2281 acagcgaaac catatcggga tagccaatag cggcggcaag agaggagttt ttgacgatgt
     2341 tgagatactg gctggttaac ggtggaataa tcacccgcag tgcctgggga ataatgacct
     2401 ggcgtagcgt aaccgggttg ggtaatccca gtgaccgagc cgcttcatgt tgaccataag
     2461 gcactgcctg gatccccgcg cggataatct cggcgataaa tgcagaggta taaaccgaaa
     2521 gtgccagcgt taaggctgcc agttcaggaa ttaaaaccat cccgccgcgg aagttaaagc
     2581 cttgtagggc tgggacatcc cagtgtagtg ctgcgccaaa aagccattgc gccagtaaag
     2641 gcaaaccgat gatcaacact gcggcgatgg gccaggtgcg gcgcagctgt ccggtcttta
     2701 tctggtatgt tttattaaag cggaatagcc caacagaaag gactatagcc ataacaacag
     2761 ccaggataaa cgcaataaat ccgtctccca gctgcggtga cggaatataa agcccacgat
     2821 tgctcaaaaa ggccagatca aacgcgctca cagcctggcg tggtccgggc aaattgcgca
     2881 acacggcaaa gtaccagaag aagatttgca gcagcggggg aatattacgg aagatctcaa
     2941 tataaattgt ggaaagcttt cgtagcagcc agttatccga aagtctcgcc agaccgataa
     3001 agaagcccaa aacagaagcg aacacaatac acaatgcaga aaccagtagc gtattgagta
     3061 agccgacaat aaaaacgcgt ccgtaggtgt cgccctgctg gtaatcgatc aaatgctgga
     3121 caataccgaa gccagcgccg cgatccagaa aggcaaaacc tgaagtaatg ccacgattat
     3181 tgagattcgt tacagtgttg tgaaataacc aaccaacaat gccgacaaca gcaacaacgg
     3241 caaggatctg gaataaccag gcgcgaaccg tagggttggc aaaagagagt gagcctttaa
     3301 cggttgagcg gcgatgagac atagaaacct cggaaagcat aacgctgaag agagcgccgc
     3361 cgtagcgacg ctcgttacct cacaccgact taacgcacgg gcggtgcgta ctgaataccg
     3421 ccgttattcc agagattatt ttgcccacgt ttaattttca gcgggctttc tgaacctacg
     3481 ttacgctcga aaatttccga gtagttaccc acctgtttga tgatgttata ggcccattta
     3541 ttatccaact tcagatcctt gccgtaatcg ccttctttac ccagcagatg tgccatatca
     3601 ggcgttgctg gattagccgc tttttcatcg acgttctggg aattgatgcc catctcttca
     3661 gcatttagca tggcgaaaag cgtccagcgt acaatcgaga accattcatc atcgccacga
     3721 cgaactaccg gcccaagcgg ttctttagag ataacctccg gtaagacaat ccattcagcc
     3781 gggttgctta atttgatgcg cagggcatac agttgggatt gatccgaagc cagcgtatcg
     3841 cagcgaccgg attccagtgc cttcgctgat tcgtcagaac gatcgaaagt cactggtgtg
     3901 tacttcatat tgtttgcctt gaaatagtcg gcgacgttga gttcggtatc agtacccgcc
     3961 tgaatacaga cggtagcgcc atccagttct ttcgcgcttt ttagccccgc tttatcgtgc
     4021 gtcaggaagc caatgccgtc gtaataagtg acgccagtaa atgccattcc catcccggca
     4081 tcgcgagatg aagtccaggt cgtattacgg gagagcaaat ccacttcccc tgactgtaaa
     4141 gccgtgaagc gttcttttgc tgtgagtggg gtatatttca ctttcgtgtc gtcaccaaat
     4201 acagcagcgg caacaccacg acaaacatca acatcaatac ctgaaaactt accgtctgca
     4261 tcggcataag agaacccagg taatccatca ctgatcccgc attgcacaaa accttttttc
     4321 tgaacggcat caagcgtcgc gccagcatgc gcctgatttg caacggcaag cagcacgctg
     4381 gcggcagcca gtgtggctat catcatcttt ttcataatgc ttcctgtgag gcgaaaatta
     4441 tcgttatagg acgtttggga aacgtctttt gcctgtctgt ggccatcctc ttataagcaa
     4501 aagtagtgcc agcttttgcg cacgatttcg cgtcgcaata agctgagtgt agacaggaaa
     4561 taataaaaac agcgccccgc aatggtgcga aaatgaaaca tgcgccatat tttggcgcaa
     4621 aacatttacg cgtgttttac taagcagaat aatgattgtt agcgtgagta tatttctggt
     4681 gggaagtgcg aaagttaaat tgatgcgtta tttcgaaagc agcgaaataa cacgatataa
     4741 catgctaaat cagcagagaa ttagccagca gaatatagca atattttatc ttatgtaaag
     4801 atactgtatc gttaaacaga gcgcgaaact ccgcgccctg ttcagttatt tcgacagagc
     4861 gatgatcccc atcgtcaggc cagcaacagc cgctgccccg ccagcaatag ccccagccgt
     4921 ttcccagtta tcctgagtcg tgcctttcat acaggtattg gtgtgaacca gacgaccctg
     4981 atcgtcataa accggaacgc agggcgaatc gtgagcacag cccgccagca atgcggtgag
     5041 caatgccaca ggaattaatc ttttcatggt gttacctcag taataaacgc taacgcttaa
     5101 taagttaact ctcttattaa cgagctgaat gccattttat caccggaggt tataaatctt
     5161 gcggaatgaa taatgacaaa ttatgtgaat tgtaaaaata gtgaagggag cgggagctat
     5221 agaaagcttt taaagagtga gcaaaagaat atcttattaa tttttatggg tttcactgga
     5281 aaacataaag gcacccgaaa gcgcctttat gtttcagatt tatcctttaa agcaacggcg
     5341 gatcaccaca aagaacaccg gtacgaagaa gattgccagc aacgttgcag agaccattcc
     5401 tcccattacc ccgataccca ctgcgttctg cgcgccactg ccggcaccgt tactgatagc
     5461 cagcggtaat acgccgagaa taaaggcgag agaggtcatc aggataggac gcagacgcat
     5521 acgtactgcc attagtgtcg cttcaacaac acctttaccc tctttctcca tgagatcttt
     5581 agcgaactca acaatcaaaa tagcgttttt ggccgacaag ccaattgtcg ttagcaagcc
     5641 taccataaag tagacgtcat ttttttgatt aaagagtgtc gccgccagca gcacgccgac
     5701 aatccctaac ggcacgacca acataaccga gacaggaatt gaccagcttt catagagtgc
     5761 agcaaggcac aggaaaacaa ccacaaagga aattgctacc agagcgggag cctggtttcc
     5821 cgataagcgt tcctgatacg acatacccgt ccagtcataa ccaatgcccg caggtaattt
     5881 tgacgctagg ttttccatca acgccatggc atcgccggaa ctggttcctg gcgcggcttc
     5941 cccctgaatc tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac
     6001 ccaatgtgaa gtggtaaagg cagagaatgg caccatttcg ccgttggtgc tgcggacata
     6061 aagtttatcg acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt
     6121 tttcacgcga ccacggtcga tgaagtcgtt aacgtaagtc ccacccaacg ccgttgaaat
     6181 ggtctgattg atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc
     6241 cagtttaaac tgcgcggtgt cttccaggcc attgggacgc acgctgacta agctggcagg
     6301 atgttgcgcc gccataccaa gcaactggtt acgggcctgg gttagggcat cgtgacccag
     6361 cccagcctta tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc
     6421 tggcatattg aatggaatga caaaaccgtc gcggatcttg cccaactcca ttttggcacg
     6481 atggattacc gcttccgcac tgttttcatc accattacgc tcttcccacg gtttcagact
     6541 gacgaaggcc atacctgtag attcaattgg tcaacgcaac agttatgtga aaacatgggg
     6601 ttgcggaggt gcctggccgc tgaagctaaa gccgttaacc gtaaagacac tttcaacgtt
     6661 tgctttctcg ttcttcagat agtaatccgt cacttgatcc aacacttttt gcgtccgctc
     6721 ttgcgtcgcg ccagcgggta actgaatcat ggtcagaaag acaccctgat cctcttcagg
     6781 taagaaggaa gacggaagac gtaaaaacaa caccaccatt cctgcaacaa tcagcgcata
     6841 gatcagtaaa tatcgtcctg tggaaccgag gattttgccg acgctgttgg tgtagtggtt
     6901 aacgctatga tcgaaggtgg tattaaacca gccgaagaaa ccacccttat tttcgtgatg
     6961 ctcagctgag gtgggtttga gcagtgttgc acataacgca ggggtaagaa tcaatgccac
     7021 cagaacagaa agcgccattg ccgaaacgat ggtgatagag aactgacgat aaattgcccc
     7081 agtagaaccg ccgaagaatg ccatcggaat aaataccgct gacagcacca tcgcgatacc
     7141 caccagtgcg ccctgtattt gcgacattga tttttccgtc gcttcttttg gcgggagctt
     7201 atcctccatc atcacgcgct cgacgttctc caccaccact atcgcatcat cgacgagcag
     7261 cccgatggca agcaccatcc cgaacatcgt tagtgtgttg atggagtaac caaaagcggc
     7321 gaggatggca aacgtcccta acaacaccac gggtaccgca atggtgggga tcagcgttgc
     7381 tcgcatattc tgcaagaaca gatacatcac caggaacacc agcataatag cttcgaacag
     7441 tgtttttacc acttcgtgaa tagaaagctg gacgaatggc gtggtgtcat aagggtagag
     7501 aaccttcatt ccctgcggga agaatggctg taattccgcc agttttgcct taatggcttt
     7561 cgcggtatcg agagcattcg cgccggttgc cagcttaata cccaggcccg ccgccggttt
     7621 tccgttgatg cgagcgataa cgttatagtt ttcaccgcca agttcaaccc gtgcgacatc
     7681 tttcagacgc accaccgagc cgtcactgtt tacgcgcagg gtcactttgc cgaattcttc
     7741 cggatttttt aaccgcgtct gagcaataat cgaggcattc aactgttgcc ctggtaacgc
     7801 tggcgttccg cccaactgtc cggcagcgat ctgagcgttc tgtaccttca actggttaat
     7861 cacatcaacc ggtgtcagtt tatatttgtt tagcagatcg gcgtccagcc agatacgcat
     7921 cgcatactgt gcgccgaaaa gctgtacgtc accgacgcta ttcagacggc taagcgtatc
     7981 tttaacgtta gaggccacat agtccgagat atcgtcctgt gtggtgtctg ggttatcaga
     8041 gacaaagcct gccaccatca aatagctgct actggacttt tcaacactga tcccctgctg
     8101 ctgaacctcc tgcggcagca acggcgtggc gagctggagt ttgttctgca cctgcacttg
     8161 cgcgatatca ggatcggtcc cggactggaa ggtaagggta attgtcacgc taccggcgga
     8221 atcgctggtg gaggacatat acatcaggtt atcgataccg ttcatattct gttcgataac
     8281 ctgcgtcacc gtatcctgca cggtctgcgc atcagcgccc ggatagtttg ctgaaacaga
     8341 aaccgcaggc ggtgcaatgg ttggatactg agcgacgggc aattgtagga tcgccagtgc
     8401 gcccgccatc atcagaataa tggccagcac ccatgcaaat atcggtcgtc gaataaaaaa
     8461 gtttgccatg tcagattacc ttacttcgat gcagtatctg cgggggtatc ggtagtggct
     8521 ttaacctgct ctcccggacg cgctttttgc aggccgctga caatgacttg atcgccagat
     8581 ttcagacctt cgctaatcaa ccatttatcg ccaatcgcct gactggcaac gacagggcgc
     8641 acttccacct gacttttatc gttaacaatc agcacggttg catcaccacg cggtgtgcgg
     8701 ctaacgcctt gttgcgggat gagaatggcg tcaggttgga cgccttcatc aatccgtgca
     8761 cgcacaaaca tacccggcaa aagcgtatgt tgcgggttag ggaagacagc acgtagggtt
     8821 atggagccgg tgctttcatc aacggtcaca tcggagaatt gcagcgtacc tttcagggga
     8881 taggtttgac cgttttccat gaccaactct acgttgctgg tggcgttttc cttatgcaaa
     8941 tttccttgct ctacggactg cttcagcctc ataaaatcgt tgctggattg ggtcacatca
     9001 acatagatag gatcgagctg ctggacagtt gccagttcag tcgtttgccc attagtgaca
     9061 agagcgcctt cggtcacagt cgatttgccg atacgtccgc taattggcgc agtcactttg
     9121 gtataagcaa gattgatgcg agcgctttcg actgtggctt ttgcggcaat cacggcggca
     9181 tcggcctggc gagcatcagc aatggcctgg tcgtactcct gctggctgat gtatttcgta
     9241 cccacgagcg gaacgtaacg ttttaccgtc aaatgcgcga tggcggcggc ggcttcactt
     9301 ttcgccagtt cgcctttcgc gctgtcataa tttgcctgat aggtcgcggg atcgatctgg
     9361 tacagggact ggcctgcttg cacatcgctg ccttcagtga aattgcgatt cagtacgatc
     9421 ccgctaacct gtgggcgaac ttcggctata cgataagcat tggtgcggcc tggtaattca
     9481 gtcttaactt ctaacggggc cgtttttaca atatgaacgg taacctgcgg ttcaccgacg
     9541 tgagctttct cttctccctt atcgttacaa ccggcgatta aagccgcgga gatcagaata
     9601 aaggagggca ggaggaaaaa cctggcatgt ttcgtcatta ctattcctca aaaaaccaaa
     9661 agcgcgttat ttacccaaaa aggcaacgcg ttaactcgca gaaagaaaaa tacagttcga
     9721 tatcctacaa attatcattc gtcgatgtaa ggaatagtta tgaatacagg cgtctcaagg
     9781 cacataaaca caaaaaaaga ttaatattct actgttttat tttgacgcgg gttgaaagag
     9841 gcagaattaa aaaatcgtaa attgaagtat atattgatgt agtgaatgta tcttaagtaa
     9901 ataatatata ttatttattt aacgatttat a
//
LOCUS       DAZFBR010000147         9768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFBR010000147 DAZFBR010000000
VERSION     DAZFBR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.147"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            64..138
                     /gene="tnaC"
                     /locus_tag="ACHJZ2_004290"
     CDS             64..138
                     /gene="tnaC"
                     /locus_tag="ACHJZ2_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO4570287.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            359..1774
                     /gene="tnaA"
                     /locus_tag="ACHJZ2_004291"
     CDS             359..1774
                     /gene="tnaA"
                     /locus_tag="ACHJZ2_004291"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO4570288.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1865..3112
                     /gene="tnaB"
                     /locus_tag="ACHJZ2_004292"
     CDS             1865..3112
                     /gene="tnaB"
                     /locus_tag="ACHJZ2_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO4570289.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3244..4419
                     /gene="mdtL"
                     /locus_tag="ACHJZ2_004293"
     CDS             3244..4419
                     /gene="mdtL"
                     /locus_tag="ACHJZ2_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO4570290.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            4394..5353
                     /gene="yidZ"
                     /locus_tag="ACHJZ2_004294"
     CDS             4394..5353
                     /gene="yidZ"
                     /locus_tag="ACHJZ2_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO4570291.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5510..6259
                     /gene="yieE"
                     /locus_tag="ACHJZ2_004295"
     CDS             5510..6259
                     /gene="yieE"
                     /locus_tag="ACHJZ2_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO4570292.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6281..6847
                     /gene="yieF"
                     /locus_tag="ACHJZ2_004296"
                     /gene_synonym="chrR"
     CDS             6281..6847
                     /gene="yieF"
                     /locus_tag="ACHJZ2_004296"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO4570293.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6901..8238)
                     /gene="adeP"
                     /locus_tag="ACHJZ2_004297"
     CDS             complement(6901..8238)
                     /gene="adeP"
                     /locus_tag="ACHJZ2_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO4570294.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            8404..9069
                     /gene="yieH"
                     /locus_tag="ACHJZ2_004298"
     CDS             8404..9069
                     /gene="yieH"
                     /locus_tag="ACHJZ2_004298"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO4570295.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9206..>9768)
                     /locus_tag="ACHJZ2_004299"
     CDS             complement(9206..>9768)
                     /locus_tag="ACHJZ2_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO4570296.1"
                     /translation="ESHKESATNVTFDYKELAPIFEGVEEKQIKALSNQLEHIKSFKT
                     DYDSRLNKYARDFYYFSSAFAINWEDLLKNYQEIRASIKDYDDLLREIKELIISRNKS
                     LEDKRTLFDNKRDNWNSIEVQDEVNALNAKIVDCDDKIRSKLTIVRNNRLENQYKDDK
                     NISDAMRNILDWFERYPDIVQNITQA"
BASE COUNT         2422 a         2403 c         2378 g         2565 t
ORIGIN      
        1 cataatagac aaatttcttg agtattatac tgtagcttcg tgtctttcga ggataagtgc
       61 attatgaata tcttacatat atgtgtgacc tcaaaatggt tcaatattga caacaagatt
      121 gtcgatcacc gcccttgatt tgcccttctg tagccatcac cagagccaaa ccgattagat
      181 tcaatgtgat ctatttgttt gctatatctt aaatttgcct tttgcaaagg ccatctctcg
      241 tttatttact tgtgttagta aatgatggtg cttgcatata tacatggcga attaatggat
      301 attgcagatg caatattcac agggatcact gtaattaaaa taaatgaagg attatgtaat
      361 ggaaaacttt aaacatctcc ctgaaccgtt ccgcattcgt gttattgagc cagtaaaacg
      421 taccactcgc gcttatcgtg aagaggcaat tattaaatcc ggtatgaacc cgttcctgct
      481 ggatagcgaa gatgtgttta tcgatttact gaccgacagc ggcaccgggg cggtgacgca
      541 gagtatgcag gccgcgatga tgcgcggcga cgaagcttac agcggcagcc gcagctacta
      601 tgcgttagcc gagtcagtga aaaatatctt tggttatcaa tacaccattc cgactcacca
      661 gggccgtggt gcagagcaaa tctatattcc agtactgatt aaaaagcgcg agcaggaaaa
      721 aggcctggat cgcagcaaaa tggtggcgtt ctctaactat ttctttgata ccacgcaggg
      781 ccatagccag attaacggct gtaccgtgcg taacgtctat atcaaagaag ccttcgatac
      841 gggcgtgcgt tacgacttta aaggcaactt tgaccttgag ggattagaac gcggtattga
      901 agaagttggt ccgaataacg tgccgtatat cgttgcaacc atcaccagta actccgcagg
      961 cggtcagccg gtttcactgg caaacttaaa agcgatgtac agcatcgcga agaaatatga
     1021 tattccggtg gtaatggact ccgcgcgctt tgctgaaaac gcctatttca tcaagcagcg
     1081 tgaagcagaa tacaaagact ggaccatcga gcagatcacc cgcgaaacct acaaatatgc
     1141 cgatatgctg gcgatgtccg ccaagaaaga tgcgatggtg ccgatgggcg gcttgctgtg
     1201 catgaaagac gacagtttct ttgatgtgta caccgagtgc agaacccttt gcgtagttca
     1261 ggaaggcttc ccgacgtatg gcggcctgga aggcggcgcg atggagcgtc tggccgtagg
     1321 tctgtatgac ggcatgaatc tggactggct ggcttatcgt attgcgcagg tgcagtatct
     1381 ggtcgatggt ctggaagaga ttggcgttgt ctgccagcag gcgggcggtc acgcggcatt
     1441 cgttgatgcc ggtaaactgc ttccgcatat cccggcagat cagttcccgg cacaggcgct
     1501 ggcctgcgag ctgtataaag tcgccggtat ccgcgcggta gaaattggct ctttcctgtt
     1561 aggccgcgat ccgaaaaccg gtaaacaact gccatgcccg gctgaactgc tgcgtttaac
     1621 cattccgcgc gcaacatata ctcaaacaca tatggacttc attattgaag ccttcaaaca
     1681 tgtgaaagag aacgcgtcga atattaaagg gttaaccttt acctacgaac caaaagtatt
     1741 gcgtcacttc accgcaaaac tgaaagaagt ttaattaata ctactgagtg gctataagga
     1801 tgttagccac tctcttaccc tacatcctca ataacaaaaa tagccttcct ctaaaggtgg
     1861 catcatgact gatcaagctg aaaaaaagca ctctgcattt tggggtgtta tggttatagc
     1921 aggtacagta atcggtggag gtatgtttgc tttacctgtg gatcttgccg gtgcctggtt
     1981 tttctggggt gcctttatcc ttatcattgc ctggttttca atgcttcatt ccgggttatt
     2041 gttattagaa gcaaatttaa attatcccgt cggctccagt tttaacacca tcaccaaaga
     2101 tttaatcggt aacacctgga acattatcag cggtattacc gttgccttcg ttctctatat
     2161 cctcacttat gcctatatct ctgctaatgg tgcgatcatt agtgaaacga tatcaatgaa
     2221 tttgggttat cacgctaatc cacgtattgt cgggatctgc acagccattt tcgttgccag
     2281 cgtattgtgg ataagctcgt tagccgccag tcgtattacc tcattgttcc tcgggctgaa
     2341 gattatctcc tttgtgatcg tgtttggttc tttcttcttc ctggtcgatt actccattct
     2401 gcgcgatgcc accagctcca ctgcgggaac gtcttacttc ccgtatatct ttatggcttt
     2461 gccggtgtgt ctggcgtcat ttggtttcca cggcaatatt cccagcctga ttatttgcta
     2521 tggcaaacgc aaagataagc taatcaaaag cgtggtcttc ggttcgctgc tggcgctggt
     2581 gatttatctc ttctggctct attgcaccat ggggaatatt ccgcgagaaa gctttaaggc
     2641 gattatttcc tcaggcggca acgttgattc actggtgaaa tcgttcctcg gcaccaaaca
     2701 gcacggcatt atcgagtttt gcctgctggt gttctccaac ttagctgtcg ccagctcgtt
     2761 ttttggtgtc acgctgggat tgttcgatta tctggcggac ctgtttaaga ttgataactc
     2821 ccacggcggg cgtttcaaaa ccgtgctgtt aaccttcctg ccacccgcgt tgttgtatct
     2881 gatcttcccg aacggcttta tttacgggat cggcggtgcc ggactgtgcg ccactatttg
     2941 ggcggtcatt attcccgcag tgctggcaat caaagctcgc aaaaagtttc ccaatcagat
     3001 gttcacggtc tggggcggca atcttattcc ggcgattgtc attctctttg gtataaccgt
     3061 aattttgtgc tggttcggca acgtctttaa cgtgttacct aaatttggct aaatccttca
     3121 agaagccagt cattcgctgg cttcttgcct ctcaggaaat cacttatgtc caaatggcaa
     3181 ctcgcctgat cctccttcac cacgtatgct ttgcgtcacc ttactatcag gacgctttag
     3241 cccatgtccc gctttttgat ttgtagtttt gccctggttt tactttatcc cgctgggatt
     3301 gatatgtacc tcgttggttt accgcgcatc gccgccgatc tcaatgccag cgaagcgcag
     3361 ttacatattg cgttctccgt ttatctggca ggtatggcag ctgcgatgtt atttgccggt
     3421 aaagtggccg atcgttcagg gagaaaaccg gtcgccatcc ccggcgcggc gctatttatt
     3481 atcacttcgg tgttctgttc actggctgaa accagcacgt tatttcttgc agggcgcttt
     3541 ctacaggggt tgggcgcggg ctgttgttac gtggtggcat tcgccatttt gcgcgacacg
     3601 ctggatgatc gacgtcgggc aaaagtgctg tcgttactca acggcattac ctgcatcatt
     3661 ccggtgttag cgccggtgct cggtcatctg attatgctta aattcccgtg gcagagtctg
     3721 ttctggacga tggcaataat gggcatcgcg gtactgatgt tgtctttgtt tattttaaaa
     3781 gaaacgcgcc ctgccgcccc cgcagcttcg gacaaatcac gagaaaatag cgagtcgctg
     3841 cttaatcggt ttttcctcag ccgtgttgtt atcaccaccc tcagcgtttc ggtgatcctc
     3901 accttcgtca atacatcgcc ggtattgctg atggaaatca tgggttttga gcgcggagaa
     3961 tacgccacca ttatggcgtt gaccgctggc gtcagcatga ccgtttcatt ttccacgcca
     4021 tttgcgctgg gaatttttaa gccacgtacg ttgatgatca cctcacaggt gttattcctg
     4081 gcggcgggca tcactcttac cgtttcacct tcccatgcgg tttctctgtt tggtatcacg
     4141 ctgatttgcg ccggtttctc ggtaggtttt ggtgtggcga tgagtcaggc gttagggccg
     4201 ttttcattac gcgcgggcgt agccagctcg accttaggta ttgcgcaggt ttgcggttcg
     4261 tcactgtgga tttggctggc agcggtggtt ggtatcagcg catggaatat gctgatcggg
     4321 attctgattg cctgtagcat agtgagcctg ttgctgatta tgttcgtcgc gcctggacgc
     4381 ccggttaccg ctcatgaaga aatccatcac cacgcttgat ctcaatctgc tgctctgtct
     4441 gcagctgctg atgcaggagc gcagcgtaac caaagcggcg aagcggatga acgtgacacc
     4501 ttcggcggtg agtaagtcgc tggcaaagtt aagagcgtgg tttgacgacc cgctctttgt
     4561 gaactcaccg ttggggctgt cgcccacacc gctaatggtc agcatggagc aaaatctggc
     4621 ggagtggatg caaatgagca acctgctgct ggataaaccg caccaccaga caccgcgcgg
     4681 cctgaagttt gagctggcgg cagaatcacc gctaatgatg atcatgctta atgcgctgtc
     4741 gaaacagatc taccaacgtt acccgcaggc gaccatcaaa ttacgtaact gggattacga
     4801 ttccttagat gccattactc gtggtgaagt ggatatcggt ttttccggtc gcgaaagtca
     4861 tccccgttcg cgggagctgt taagctcgct accgttagcc attgattatg aagtgttgtt
     4921 tagtgatgtg ccctgcgtct ggttacgcaa agatcatccg gcactgcatg aaaagtggga
     4981 tctcgatacc tttttacgtt atccgcatat cagcatttgc tgggaacaga gcgatacctg
     5041 ggcgctggac aatgtgttac aggagctggg gcgcgaacgt acgattgcaa tgagcctgcc
     5101 ggaattcgag cagtcactgt ttatggcagc gcagcccgac aatctgctcc tggcgaccgc
     5161 gccgcgctac tgtcagtact acaatcaact ccaccaactg ccgttggttg ctcttcctct
     5221 cccgtttgac gaaagccagc aaaaaaagct ggaagtccct tttaccctgc tgtggcataa
     5281 acggaacagc cataatccga agatcgtctg gttacgggat accattaaaa atctttacgc
     5341 gtcgatggca taaccgaatc gtatgaaacg cgacccgatt tcacaataaa atgtaaaaaa
     5401 gttgtaataa gcttgtctga atcgaacttt tagccgcttt agtctgtcca tcattccagt
     5461 aaatgattac tcttgtattc ataatggacc attaagcatg gagcgaaaaa tggcgactca
     5521 ctttgcccga gggattttaa cggaaggaca tctgatttct gttcgtctcc cctcccagtg
     5581 tcatcaagaa gcccgaaaca ttccccctca tcgtcaaagc cgttttctgg cgtccagagg
     5641 tttactcgca gaactgatgt tcatgctgta tggcattggc gaattgccgg aaatcgtcac
     5701 cctgccgaaa ggtaaaccgg ttttcagtga taaaaatttg ccttcgtttt ctatttccta
     5761 tgccgggaat atggttggcg tggcgttaac aaccgaaggt gaatgtggcc tcgatatgga
     5821 actgcagcgt gcgacgcgcg ggtttcatag cccacacgcg cccgataacc acacattttc
     5881 cagcaatgaa tcgctatggg tcagtaaaca aaatgatcca aacgaagcgc gggcgcagct
     5941 catcacgctg cgccgaagcg tgctaaaact aaccggtgat gttttgaatg acgatccgcg
     6001 cgatctgcaa ctgctgccca ttgccggacg cctgaaatgt gctcatgtaa atcatgtaga
     6061 agcgttatgc gacgcggagg acgtgctggt gtggtccgtg gcggtcacgc ccacgattga
     6121 aaagctcagt gtctgggagt tagatggcaa acacggctgg aaaagcctgc cggatattca
     6181 cagccgcgcc aacaatccta ccagccggat gatgcgtttt gcccagctct ctaccatgaa
     6241 ggctttttcg ccaaattgat agacaaccac aggagtcatt atgtctgaaa aattgcaggt
     6301 ggttacgtta ctggggagcc tgcgcaaagg ctcatttaat ggcatggttg cacgtaccct
     6361 accgaaaatt gctccggcga gcatggaagt caatgcgtta ccttccattg ccgacattcc
     6421 cctgtatgac gctgacgtac agcaggaaga aggttttcca gcaacggttg aagctctggc
     6481 ggaacagatc cgtcaggctg acggtgtggt gatcgtcacg ccggaatata actattcggt
     6541 accgggtggg ctgaaaaatg ccatcgactg gctttcccgc ctgccggatc aaccgctggc
     6601 cggtaaaccg gtgttgattc agaccagctc aatgggcgtg attggcggcg cgcgctgtca
     6661 gtatcacctg cgccagattc tggttttcct cgatgcaatg gtgatgaaca agccggaatt
     6721 tatgggcggc gtgattcaga acaaagttga tccgcaaacc ggagaagtga ttgatcaggg
     6781 tacgctggac cacctgaccg ggcaattgac cgcatttggc gagtttattc agcgagttaa
     6841 gatctaaata aaaaacccgc cagcaatcat gcatggcggg tttttaacgc gctatcgatt
     6901 ttagtgagcg tcgataaata caatcttcag gataaacagc agcgcaacga tgattacgca
     6961 cgggctaagg tcacgaatac gcccggtacc aatcttcatc acgcagtagg agataaagcc
     7021 cagcgcaata ccttcggtaa tcgagaagct gaacggcatc atcacagcgg taataaacgc
     7081 cggaacagat tcagtaagat cctgccagtt cacgcgagca agacttgagg tcatcaacac
     7141 gccaacgtaa atcagcgcgc cagctgcagc gtagcctggc accattcccg ccagcggcga
     7201 cagaaagata accagcagga acagcagacc aacaaccact gccgtcagac cggtacgacc
     7261 gccaaccgat acgccggaag aggactcaat ataagccgta acggaagaag taccgataaa
     7321 cgaaccggtc acggaagaga tactgtcgac atacagcgcc tgcttcatgc gcgggaattt
     7381 ccccttctca tctgccagac cggctttatc ggtcacgcca atcaacgtgc cggaggagtc
     7441 aaacaggttg accagcatga aagagaaaat cacccctgcc agcccgaggt taaacgaccc
     7501 ggctaaatct acatgaccca cgacagtcat tacgctcggc ggcgcagaaa cgatgccatt
     7561 gtagtggaca tcacccagca tccagcccag cagcgttgtc accacgatag aaaccagcac
     7621 cgctgcgtga atgttgcgtg aggccagaat agcaatgatg aagaagccga ggatccccag
     7681 cagcacgctg tgagaagtca gattaccgat gctgaccagc gtttccgggt tagcgacaat
     7741 cacacctgcg tttttcagcc ccatcatgcc aatgaacaga ccgataccgc tggtaatgcc
     7801 cacacgcaga ctcaccggaa tgttggcaat catccagtag cgaacgcgga aaatcgtcag
     7861 taacagcaga ccaatcgcgc cccagaagat tgcgcccatc ccgacttgcc acggcaagcc
     7921 catcgcctgc acaacgacaa aagcgaagaa cgcattcagg cccatagcgg gtgccagtgc
     7981 aactggcagg ttagcaaaca gccccatcat aatactgccg aatgcagcaa tcagacaggt
     8041 agtgacgaag acggcgctgg tatccatgcc agcaacgcca agaatttgcg ggttaacaaa
     8101 aacgatgtaa accatcgtca ggaaggtggt aaaaccggcg atcacttcgg tccgtgccgt
     8161 cgtgccatgt tcgcgcagtt taaacacgcg ttccagcatc ccctggccag aagcctgggt
     8221 tgtgtgttgt tgactcatta tctatttccg aacaaggagg gaaaatccgt cgctatcgta
     8281 taccaaaatg agacaatatg gacggctgaa gataactttt ttcattgtat ttgcttatac
     8341 ggcaacgatt gcgtcatgcg aattggcatg acgaaaacgt taaactaacg ataaaggaaa
     8401 gacatgtccc agatagaagc ggtatttttc gactgcgacg gtacgctggt cgatagcgag
     8461 gtcatttgct ctcgcgccta tgtcacgatg ttcagggaat ttggcattca cgttgacctg
     8521 aaagagattt ttacccgttt taaaggcgta aaactctacg aaatcatcga tatcatcagt
     8581 aaagaacagg gcgtgacgat ggtccaggcc gatgccgagc acgtttaccg cgcagaagta
     8641 gcacgtctgt ttgataccga acttgaggac atcgcaggag cgaacgaatt gctggcgagc
     8701 attaacgtgc cgatgtgcgt cgtctctaac ggtccggtca gcaaaatgca gcattcgctt
     8761 ggtaagctca acatgctgca ctacttcccg gaaaaactgt tcagcggata tgacattcag
     8821 cgttggaagc ccgatccggc attaatgttc catgcggcaa aagcgatgaa tgtgaacgtt
     8881 gaaaactgca ttctggtcga tgactccagc gctggcgctc aatcagggat agatgccgga
     8941 atggaagtgt tttacttctg cgctgacccg cacaataagc cgatcgttca cccgaaagtc
     9001 accaccttta ccgagctgtc gcagctgcct gcgctctgga aggcacgcgg ctggaatata
     9061 acgcgataat cgcctcctta cttcaacact tacacatcag tcacgctggt ttgagataca
     9121 gaacaccagg tgtctgatga gtaagcgtct attccctatg aataatttat gtcgttattc
     9181 gtcctgtcat ccaccctctg tatggttaag cctgggttat attttgtaca atatcgggat
     9241 accgttcgaa ccaatccaga atattacgca tcgcgtcact gatgttttta tcgtctttat
     9301 attgattctc aagtctatta tttcgaacaa tagtaagctt agaacgtatt ttatcatcac
     9361 aatcgacaat tttcgcattc aatgcattta cttcatcctg cacttctata ctattccaat
     9421 tgtccctttt gttatcaaat aaagtccttt tatcctctaa tgatttatta cgacttatga
     9481 ttaactcttt aatttctcgc aataagtcat cgtaatcttt aatggaagct cttatttcct
     9541 gatagttttt tagtaaatcc tcccagttaa ttgcaaatgc actggaaaaa tagtaaaaat
     9601 cgcgagcata cttattgagc cttgagtcgt aatctgtctt aaacgatttt atatgttcca
     9661 gttggtttga tagtgcttta atttgttttt cttcaacacc ttcaaaaatg ggtgcaagtt
     9721 ccttataatc aaatgttaca tttgtagcag attctttatg agactcct
//
LOCUS       DAZFBR010000148         9597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFBR010000148 DAZFBR010000000
VERSION     DAZFBR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.148"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..759
                     /locus_tag="ACHJZ2_004300"
     CDS             <1..759
                     /locus_tag="ACHJZ2_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4570297.1"
                     /translation="RQSKLTKLMSETMSINPFMTPFIFDYHSLNDFDELVEAIIAKHL
                     MTGHDTGFGKLIDEKILPRVFGAYKLDKSYRAANEPFIHPCFDEIDHVIQRDDGRIEL
                     LSLKAGKWTIQLTMAVQLNKAFHEIINNYPGVADNIVVGVFYGNSHGLTDKYRILRGI
                     NTGANHNVIDIRDKVHVYAGKEFWSWLNNGEAETQHWVLEGIERAVKEADIKEKNKDL
                     IEKFKEHVAKKYNEQVLNADGTAQWHKLLEMINE"
     gene            complement(797..2041)
                     /locus_tag="ACHJZ2_004301"
     CDS             complement(797..2041)
                     /locus_tag="ACHJZ2_004301"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO4570298.1"
                     /translation="MSSKKFISLFSGAMGLDLGLEEAGFELVACVEQDKAALKTIKTN
                     KPNLAVFEGSIVDCTGSELLALAGVNDKEEIDLVAGGPPCQAFSVFGNRLGLEDARGQ
                     LIFEYVRMIKELNPKVFVMENVRGLLSMSIVPASKKKELSGTIAASAFEKGSLIKRVA
                     NEFNKLGYHVDCFVVNAANYGAPQIRERVILIGNRFGMIANFASPLFSNRVEDNLPPF
                     KTLGDVIGPESGFVDKYPEVMNFSPRKLKYLSMVPPGGNWRSMPEEMQKESMGKSWYL
                     KGGRSAYWRKLSFQFPSPTVVTMPNHAGTSMCHPTELRAISVGEAAAIQEFPDYWKFE
                     GTTTEKFRQIGNAVPVRLGKVAGEAAMELLHRIQENKVADLVTPDYIETHIRPHIRTR
                     SFFKNGQAYSGDVSYYDLEEEV"
     gene            complement(2367..2939)
                     /locus_tag="ACHJZ2_004302"
     CDS             complement(2367..2939)
                     /locus_tag="ACHJZ2_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO4570299.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(3013..3513)
                     /locus_tag="ACHJZ2_004303"
     CDS             complement(3013..3513)
                     /locus_tag="ACHJZ2_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO4570300.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDSKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAERLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(3510..4244)
                     /locus_tag="ACHJZ2_004304"
     CDS             complement(3510..4244)
                     /locus_tag="ACHJZ2_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFO4570301.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSIQVQENPLAN
                     TTGHQGYVAPERAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            4796..5062
                     /locus_tag="ACHJZ2_004305"
     CDS             4796..5062
                     /locus_tag="ACHJZ2_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4570302.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            5059..5649
                     /locus_tag="ACHJZ2_004306"
     CDS             5059..5649
                     /locus_tag="ACHJZ2_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4570303.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSMLSATY
                     DAPCVFFCVAINATERQIMVWRVVCRAGMILFAIACYATESMVAQAGQPPGWPVSDNA
                     GILTPVWAIAIERENSGDSVIYAVIGGCLMATTLTPSHPEFVFVFAAVRRADRHPRIC
                     MLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            5642..5929
                     /locus_tag="ACHJZ2_004307"
     CDS             5642..5929
                     /locus_tag="ACHJZ2_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFO4570304.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSRFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            5922..6377
                     /locus_tag="ACHJZ2_004308"
     CDS             5922..6377
                     /locus_tag="ACHJZ2_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570305.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVHILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPASRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            6513..6833
                     /locus_tag="ACHJZ2_004309"
     CDS             6513..6833
                     /locus_tag="ACHJZ2_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HFO4570306.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            6848..9181
                     /locus_tag="ACHJZ2_004310"
     CDS             6848..9181
                     /locus_tag="ACHJZ2_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO4570307.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNHCGAGDGLKLVEKVFGVSPSDAATKVAAVTGSLPPADPAVTAAA
                     GAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRCLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            9396..>9597
                     /locus_tag="ACHJZ2_004311"
     CDS             9396..>9597
                     /locus_tag="ACHJZ2_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570308.1"
                     /translation="MSHTGRMPEVKNRMFTLHPLFTTYQTEIKGENRKVNSVNSSFEK
                     KFFMWIKDALVWIRATDPNLLIK"
BASE COUNT         2303 a         2513 c         2405 g         2376 t
ORIGIN      
        1 agacaaagta aactaacaaa gttaatgtca gaaacaatgt ccataaatcc gtttatgacc
       61 ccttttattt ttgattatca ttcccttaat gattttgatg aactagttga agcaataatt
      121 gctaagcatt taatgactgg gcatgatact ggctttggaa aattgattga tgaaaaaata
      181 cttcctagag tgtttggtgc atataaatta gataaaagtt atagagctgc caacgagcct
      241 tttatacacc cttgctttga tgaaatagat catgtaatac aaagggatga tggtagaatt
      301 gagcttttat ctcttaaggc agggaaatgg actattcaat tgacgatggc tgtccaacta
      361 aataaagcat ttcacgaaat cataaataat tatcctggag tggctgacaa tatagtggtt
      421 ggggtttttt atggaaatag ccatggatta actgataagt atagaatctt acgaggtatc
      481 aatactggtg caaaccacaa tgtgattgat attcgtgaca aagtgcatgt atatgctggt
      541 aaagaatttt ggtcatggct taacaatggt gaggctgaaa cccagcattg ggtacttgaa
      601 ggtatagaac gtgctgtaaa agaagcagat ataaaagaaa aaaataaaga tcttattgaa
      661 aaattcaaag aacatgttgc aaagaagtac aatgagcaag ttctaaatgc tgatggcaca
      721 gctcaatggc acaaattact tgaaatgatt aatgaataaa aaactggcct aagggccagt
      781 ttttttatga ctaattctaa acttcttctt caaggtcgta gtaagaaaca tctccactat
      841 atgcttgacc atttttaaag aaagatctgg ttctaatatg cggtctaata tgggtttcta
      901 tataatctgg agttactaaa tcagcaacct tattttcttg tatacgatga agaagttcca
      961 tagcagcttc ccctgcaact ttacctaacc ggacaggaac tgcgttacct atctgtctga
     1021 atttttctgt agttgtacct tcaaatttcc agtaatcagg aaactcttgt atcgcggcag
     1081 cttcaccaac agaaatagct ctaagttcag ttgggtggca catacttgtt cccgcatgat
     1141 ttggcattgt tactactgtg ggtgaaggaa actggaaaga tagtttcctc cagtaagcac
     1201 ttcttcctcc tttaagatac cagctttttc ccatagattc tttttgcatt tcttctggca
     1261 tgcttctcca gtttccgcca ggtggaacca ttgaaagata tttcagtttt cttggcgaaa
     1321 aattcatgac ttctggatat ttatcaacaa aaccgctctc tgggccaatt acatccccca
     1381 gcgttttgaa tggcggaaga ttatcttcta ctctattgct gaataaaggg gaggcgaaat
     1441 tggctatcat tccgaagcga ttaccaatta aaataacacg ctccctaatt tgtggggctc
     1501 catagttggc agcattaact acaaagcaat ccacatgata acctagttta ttgaattcgt
     1561 tagctaccct ttttattaga gagccttttt cgaaagcaga tgctgcgatt gtgcctgata
     1621 gctccttctt tttagaagct ggaactatag acattgataa taaaccgcgt acgttttcca
     1681 ttacgaaaac ttttgggttt aattctttaa tcattcgaac gtattcgaaa ataagctgcc
     1741 ctctggcatc ttctagccct aacctatttc caaaaacact aaatgcttgg cacggtggac
     1801 cgccagcaac taaatctatt tcttctttat cattcactcc ggctagtgct aataattcag
     1861 agccagtgca gtcaacaata ctaccttcga atacagcaag atttggctta ttagttttaa
     1921 ttgtttttag agctgcctta tcttgttcaa cgcaagctac gagctcaaag ccagcctctt
     1981 cgagacctaa gtctaacccc attgcgccac taaatagcga aataaacttt ttactagaca
     2041 aaataatcac ctacatactt agcagccaaa tactaggata ttgtaaagga ttaacactaa
     2101 tcctcaactc aagtttcagg gatcttagca gaatctgctt tgcaaggatt ctatgaatgt
     2161 aaattaatag atactggcgc gcagtgcttt ccccgcctcg cccgcccgct ttgcagggcg
     2221 gttttaatgc agttgtactg acacgctcag actgcgccgg gaatggcgcg gtctgcagaa
     2281 aatgaggcag ggaaacgcat gcaaagccat gcaccttatc gatgcatggc ttttttcagt
     2341 aaaaacgggc ggattttcgg ggaattttac acagactgac gtgatgccag ttgcgcactt
     2401 ttacgcgaaa aaatcatgtt ctgcgcagga gtgaattttt cacggctgtc atccaccgaa
     2461 gccgcgtcag gcctgaatcc gatggtcgtt aaaatgtcgc tatcctgtgc ggaataatta
     2521 attttttccc ccttcgcaag ccaggacaga agggcttcac gcagggcatc tgtggcacgc
     2581 tgtatggcac agtttcgggc aatggccgtc agctcactgt agcccatcag ctccggtgcc
     2641 agtgccgccg ccagtgctgt gccgtgctgc tgcataaaat cattcagccg gtcgcggatg
     2701 ctgatgtgct gaacggcttc atgcgaacga atataacgac cggcggcctg attcacctgc
     2761 cattttctga cttcgataat attgcgtaat tcgtccaggc gactgacgtt tctgccttct
     2821 cctgacagaa gccgcagata ttcctgttcg gccgcagcca gctcattttt gcgttgcagc
     2881 catgctgctt tgttattctg acaggtgtca aaggcctgct gtaaggctgt gctttccatc
     2941 gttatctctt tctcatcatg ctgaagaata aaaatacggt gtgtggcgac ggccggtgtt
     3001 aaccggcagc cctcattcca gacgcagcga atatgattgt gtttttaacc gtactggcgg
     3061 cagttcctgt ttttcatgca ggcgttctgc cagttcgtcc ggcgtgaccg ggcggacaat
     3121 gaaacggttg atggtctgaa gcgttttaaa caccagacca cagcccggat ccgtgcacac
     3181 ataaaaacgc tcggtgactt cctgagacag acgccgcgat gttcttgaca gtgcaaggcc
     3241 tttacatctc cgacaacaat atccggtaac aagcattctt ttcgggcgtt tcatactgcc
     3301 ggaggctgac gtcagtgaat cgcggtatct ctgtttgcct gaaatgtatt ccattcctga
     3361 atctttactg tcagagaaaa agctttcact cgcttcaaat gtcgcagagc aataaatatt
     3421 ccggcactgt gcaatcatta tcttggtgcc atcgtccatg aaatgtgcgc ggcgggtgtg
     3481 agcaacatgt ccacacgacg ggcagtaaat catgacagca gtcctctggc cttaagctct
     3541 gctccctgct ggtctatttt gtcctgccac accttgcgct gtgccggtgt gcctgccacc
     3601 tcataatcca tgtgcgggag tgttgccgct gacagtccgg tcagccggag aaccggctcg
     3661 ccggtgaggc tgatttgcat ctgttttatt ttctgttcca gcgatgattt cacctgctgc
     3721 atgacagccc tttccggtgc gacgtagccc tgatggccgg tggtgttggc gagcggattt
     3781 tcctgtacct ggatgctcag atgcattgcc cggacaagcg cctcacaggt ttcattcagg
     3841 gcgtgttcca gctcatgctc tgcatacaga ctcagcaggt gatgatgtgc cttccggtag
     3901 gcggtggccg tgctgtcaca cgcccctttc aggcgttcac gttcgaaatt cagcaccacg
     3961 gccagattgt catattcctg taccagctcc cggcgtgcca cgcgctcaat gtggcgctgt
     4021 ttcagctcgt cgctcaggac accaccggct gcacgaaagg ccgtgcgcca gtcgtcagcg
     4081 tcgtttccgt cggcctgcgc cagcgcattt ttttcctgct ctgcccgttc aatggccgtg
     4141 acggtctcat ccatcaggcg ggcgttctca agatgggcgg ctctggcctt ttccagttgt
     4201 gccagcgcgg gttgcagata ttcagggatg gtgttgtcag acattttccg gctcctcgtc
     4261 acttcaggtt gagaaaattg tgacgtacac cggacaacaa cacgacgcat tgcagatgtg
     4321 ccagccctga cacaggagac tcatcctcag accggcaagc caggaaaagg tcgcaggaaa
     4381 aaccggctta ctgtttgttt ttttatattt tactgttcac ctctgttcac cataagaaaa
     4441 aagataagta atacagtaag ttaaagggtg aacaatcgca gtaatgactg ttcaccgtct
     4501 gttcaccact gttcacccgt tgatgggctt tttatgctgt ttattactgt ttatttttat
     4561 taattcactg aaataaataa gaaaaaacaa tttgcatttc actataaaaa attccagttc
     4621 cttcagaacc ctttgagacc cttccagtcc ggatggataa aaaacacaca gccattgtaa
     4681 ggttaccgga acaaattccc cctgttgcgt ctgctgaaaa tattcacaaa ataaagcgct
     4741 acccgaagcc ggacggactt atccggtgct gtatggacat taacgaggta gcccgatgca
     4801 agctgttttt tcttccccgt ctcccgcccc tgttacgcca ctgatgccgc tgccggacat
     4861 cacgcaggag cgttttttac gtctgccgga agtgatgcac ctgtgcggcc tgtcacgctc
     4921 gaccatctat gaactcatcc gtaaggggga atttccgccg caggtgagtc ttggcggtaa
     4981 aaatgtggcc tggctgcact ctgaagtcac cgcatggatg gccgggcgca ttgccggacg
     5041 caaacggggg tacgacgcat gatgatgccc gctctgcaaa aactcccttt ttctggcttg
     5101 cctttttccg gcatttgcgg atatagtttt tccgctgccg caaaatcggc agccgggcgt
     5161 gagaacccga gtatgttatc ggcgacatat gacgcgccat gcgtcttttt ttgtgtcgca
     5221 atcaacgcca cagagcgcca gattatggtg tggcgtgtgg tttgccgtgc aggtatgatc
     5281 ctgttcgcaa tcgcatgtta tgccactgag tcaatggtag ctcaggcggg gcagcctccg
     5341 ggctggccgg tatccgataa cgccggtatt ctcacccccg tctgggctat cgccatcgag
     5401 cgtgagaact ccggcgatag cgtcatttac gctgttatcg gaggttgcct tatggctacg
     5461 acccttaccc cctcacaccc tgaatttgtc tttgtgtttg ccgctgtccg tcgcgcagac
     5521 cgccatcccc gtatctgtat gcttcgcacc gtcgccggtg atgaacgcag cgcccgccgt
     5581 tcccttgtcc gtgactatgt gctctccctt gctgcccgtc tgccggtggt ggaggtgtcc
     5641 cgtgcgtaat aaaaaagccc ctcagaccgt ctcagcgcgt catgacgccc gtgaacacct
     5701 cagcattgag gcttaccata agctcaaccg cgccagcgcc gtatcccggt ttgttggggg
     5761 tgatttgatt caccgtgaac tctccggcct gcatcagctc tacattccgc atattttcag
     5821 ttacctgaat gaagatattg attttgtgct gaatgagctg aaagccaaag gcctgtgccg
     5881 cgattttctc gcccagcaga aagaccgggg agacaggacg catgtttgat tttccccagc
     5941 ccggtgagat ttaccgttct gccggttttc ccgatgtggc cgtggtcggc attctggaag
     6001 acggtattcc gtgggaaatg ccgtaccgct gcccggacat tgtctggaac ccgtaccgcc
     6061 gtaaattcag tatccttgtg catatcctcg ctgacgggcg caccacagac atcccgctgg
     6121 ggcgttttct gcgggaattt acctgtgacc gtcctgacct gtttaaacgc agccccgtaa
     6181 accggcatgc ggtactgaaa gaaatggccg gagacccgga attacagaaa tggcgggaga
     6241 aatatctgga tatttacccg caggacactg ttccggccag ccgggcggca ccggtggcgc
     6301 gggaatggcg ggaaattccc cgcacggagc ctgacccgga aatcaccccg gataacagtt
     6361 accgcaatta tctgtaatta aaaaacgaca cccgaaaaat taaatgtgcg tattcgcgca
     6421 gggatacgca cgtcttcagg agacgcagat atgccttatc agttaatgca accggcacgg
     6481 aatgcagtca tctgtcacag ggaggaaaac aaatgaaaac ccccttaccg cccgtcttac
     6541 gcgctgccct ttaccgtcgc gctgtcgcct gtgcctggct gaccgtgtgc gaacgtcagc
     6601 accgctaccc gcatctcacc cttgagtcac tggaggcggc catcgccgct gagctggaag
     6661 gcttttatct gcgccagcac ggtgaggaaa aagggcgtca gatagcctgt gccctgctgg
     6721 aagatttaat ggaatctggc cccctgaagg ccgcgccgtc gctgtccttt ctcgggctgg
     6781 ttgtgatgga tgaactctgt gcccgtcaca taaaagcgcc ggtactgcac tgaaggagaa
     6841 caacaccatg aaaatgaacg taaccgccac cgtcagccat gcgctcggcc actggccgcg
     6901 tattctcccg gcgctgggga ttcaggtgct gaaaaaccgt catcagccct gtccggtctg
     6961 tggcgggagt gaccgcttcc gttttgatga cagggagggg cgcggcacct ggtactgcaa
     7021 tcattgtggt gccggtgacg gcctgaaact ggttgaaaag gtgtttggtg tctccccgtc
     7081 cgacgcggcc acaaaggtgg ctgccgtgac cggcagtctg cccccggctg acccggcagt
     7141 gacggctgcc gctggtgctg aaacagacgc tgcccggaag aacgccgccg cactggcaca
     7201 aaccctgatg gcgaaaaccc gtcccggaac cggtaacgcc tacctgaccc gcaagggctt
     7261 tcccggccgg gaatgccgga tgctgaccgg cacacacaga gccggtggcg tgagctggcg
     7321 tgccggtgac cttgtggtgc cactgtatga cgacagcggc gaactggtta accttcagtt
     7381 aatcagtgct gacggccgta agcgcaccct gaaaggcgga caggtcaggg gcacctgtca
     7441 cacccttgaa ggacagaatc aggccggaaa acgtctgtgg atagcggagg gatacgcgac
     7501 cgcacttacc gtacatcacc tgaccggtga aacggtgatg gtggcgcttt cttccgtgaa
     7561 cctcctttct ctggccagcc ttgcccggca gaagcatccg gcctgtcaga ttgtccttgc
     7621 cgcagaccgt gacctcagcg gtgacggcca gaaaaaagcc gccgcagccg cagatgcgtg
     7681 tgaaggtgtt gttgccctgc cgccggtctt cggtgactgg aatgatgcct tcacgcagta
     7741 cggcggggag gccacccgta aggccattta cgatgccatc cggccaccgg ctgaaagccc
     7801 gttcgacacc atgagcgaag cagagttttc cgccatgagt accagcgaaa aggccatgcg
     7861 tatctatgag cattacggcg aggcgctcgc ggtcgatgcc aacggccagc ttctgtcccg
     7921 ctatgaaaat ggtgtctgga aggtgctgcc gccacaggac tttgcccggg atgtggccgg
     7981 gctgtttcag cgtctgcgcg cgccgttctc ctccgggaag gtggcctccg tggtggacac
     8041 cctgaagctg attattccgc agcaggaagc cccctcccgc tgcctgattg gctttcgtaa
     8101 cggcgtgctc gacacgcaga acggcacgtt ccacccgcac agtccgtcac actggatgcg
     8161 taccctgtgc gatgtggatt tcaccccgcc ggtggaaggg gaaacgctgg aaacccacgc
     8221 ccccgcgttc tggcgctggc ttgaccgtgc cgccggtggc cgtgcggaaa aacgcgacgt
     8281 gattctggcc gcactgttta tggtgctggc aaaccgctac gactggcagc tctttctgga
     8341 ggtgacaggt cccggcggca gcggcaaaag tatcatggcc gaaatagcca ccctgctggc
     8401 cggggaggat aacgccacgt cggccaccat cgagacgctg gaatccccgc gtgaacgtgc
     8461 cgcgttaact ggcttctcac tgatacgcct gccggaccag gaaaaatgga gcggcgacgg
     8521 tgccggactc aaggccatca ccggcggcga tgcggtgtcc gtggacccga aataccggga
     8581 tgcgtactcc acgcatatcc cggcggtgat tctggccgtg aacaataacc cgatgcgctt
     8641 caccgaccgc agcggcggcg tgtcacgccg gcgggtgatt attcacttcc cggaacagat
     8701 agccccgcag gagcgcgacc cgcagcttaa ggacaaaatc acccgcgagc tggcggtcat
     8761 cgtgcgtcac ctgatgcaga agttcagcga cccgatgctc gcccggtcac tgcttcagtc
     8821 ccagcagaac tcagacgagg cgctgaacat caaacgggat gccgacccga cgtttgattt
     8881 tatcggctat ctggaaaccc tgccgcagac cagcggcatg tatatgggga acgccagtat
     8941 catcccgcgt aattaccgta aatacctcta tcacgcctat ctggcctaca tggaggcaaa
     9001 cggctaccgg aatgtactca gtctgaaaat gttcgggctg gggctgccgg tgatgctgaa
     9061 ggaatacgga ctgaattacg agaagcgcca taccaaacag gggatacaga ccaacctgac
     9121 gctgaaagag gaaagctacg gcgactggct gccaaaatgt gacgaccctg caacaacctg
     9181 accctcatga cccacctgac cggcatctgc cggtcttttt ttatccctga attccccgaa
     9241 ggtgaacaat ccactgttca cccttcaccg tatattcacc agttatcaca ctgaaattaa
     9301 aagagaaaaa cgaaaggtga acagtgtgaa caatcaaatc aaaaaaaact ttttttctcc
     9361 ctgtgtgatt tcagtacggg ggattaatca ccggtatgag tcacaccggc agaatgccgg
     9421 aggtgaagaa tcgaatgttc acccttcacc cattattcac cacctatcaa actgaaataa
     9481 aaggagaaaa cagaaaggtg aacagtgtga acagttcttt cgaaaaaaaa ttttttatgt
     9541 ggataaaaga tgcactggtt tggatccgag ctacggatcc aaacttactg attaaaa
//
LOCUS       DAZFBR010000149         9575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFBR010000149 DAZFBR010000000
VERSION     DAZFBR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.149"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            81..1414
                     /gene="adeQ"
                     /locus_tag="ACHJZ2_004312"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             81..1414
                     /gene="adeQ"
                     /locus_tag="ACHJZ2_004312"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            1467..1919
                     /gene="yicN"
                     /locus_tag="ACHJZ2_004313"
     CDS             1467..1919
                     /gene="yicN"
                     /locus_tag="ACHJZ2_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO4570309.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2035..2358)
                     /locus_tag="ACHJZ2_004314"
     CDS             complement(2035..2358)
                     /locus_tag="ACHJZ2_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4570310.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2342..2695)
                     /locus_tag="ACHJZ2_004315"
     CDS             complement(2342..2695)
                     /locus_tag="ACHJZ2_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4570311.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2929..4119
                     /gene="nepI"
                     /locus_tag="ACHJZ2_004316"
     CDS             2929..4119
                     /gene="nepI"
                     /locus_tag="ACHJZ2_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO4570312.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4160..4453)
                     /gene="yicS"
                     /locus_tag="ACHJZ2_004317"
     CDS             complement(4160..4453)
                     /gene="yicS"
                     /locus_tag="ACHJZ2_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO4570313.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            4675..5493
                     /gene="nlpA"
                     /locus_tag="ACHJZ2_004318"
     CDS             4675..5493
                     /gene="nlpA"
                     /locus_tag="ACHJZ2_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO4570314.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(5497..6420)
                     /gene="yicL"
                     /locus_tag="ACHJZ2_004319"
     CDS             complement(5497..6420)
                     /gene="yicL"
                     /locus_tag="ACHJZ2_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO4570315.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(6531..6704)
                     /locus_tag="ACHJZ2_004320"
                     /pseudo
     CDS             complement(6531..6704)
                     /locus_tag="ACHJZ2_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6884..6989
                     /locus_tag="ACHJZ2_004321"
                     /pseudo
     CDS             6884..6989
                     /locus_tag="ACHJZ2_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            7000..7065
                     /locus_tag="ACHJZ2_004322"
                     /pseudo
     CDS             7000..7065
                     /locus_tag="ACHJZ2_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(7440..8258)
                     /locus_tag="ACHJZ2_004323"
     CDS             complement(7440..8258)
                     /locus_tag="ACHJZ2_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO4570316.1"
                     /translation="MKRKPLIAFFIAIFIALTILILFPFNCTYKKSPQEHSLSFIKQL
                     PNTVNLSSLTYNKEDDFLYATQNSPAQLLKITKSGDIMDRAPLPFISDAETIEHIQGN
                     IFAAVDEKTSELFFFTVTKDMHISFRNKIQLEKFNKKNRGFEGLAWKADDRMLFVAKE
                     RRPSKIFIYQLSPDLLSVKQATIPEALNDIRVNDISGLAFNNESLMILSDESRKLLKF
                     NLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVTLPDESIYVASEPDILAKFVPNK"
     gene            complement(8386..9531)
                     /gene="espG"
                     /locus_tag="ACHJZ2_004324"
     CDS             complement(8386..9531)
                     /gene="espG"
                     /locus_tag="ACHJZ2_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFO4570317.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQPRYAASETYLQNT"
BASE COUNT         2438 a         2008 c         2279 g         2850 t
ORIGIN      
        1 atgtgataat tatcactgaa ccgttgttgt catttgtttt acaaaagcaa gggattaaat
       61 ctcctccaat ggacaaaaaa atgaataatg acaataccga ttacgtgagt aatgaatcag
      121 ggacgctttc gcgattattt aaactacctc agcatgggac caccgtccgc acagaattga
      181 ttgcggggat gaccactttt ttaaccatgg tgtacatcgt ttttgtgaac ccgcaaatcc
      241 tcggcgcggc acaaatggac ccgaaagtgg tgtttgttac cacctgtttg attgccggta
      301 tcggcagtat tgcgatgggg atatttgcta acttacccgt ggcgctggct ccggcaatgg
      361 ggctgaacgc cttctttgcg ttcgtggtcg tgggggcgat gggtatctcc tggcagaccg
      421 ggatgggcgc gatattctgg ggcgcaattg ggctattttt gctcacgcta tttcgtatcc
      481 ggtactggat gatctccaac attccattaa gtttacgtat tggtatcacc agcggaatcg
      541 gattatttat cgccttaatg ggattaaaaa atactggcgt tattgtcgcc aataaagaca
      601 ctctggtgat gattggcgat ttaagttctc acggcgtgtt gttaggtatt ttagggtttt
      661 ttattataac cgtgttgtca tcacgtcatt ttcatgccgc ggtgcttgtt tctattgtgg
      721 tgacgtcttg ctgtggatta tttttcggtg atgttcattt tagcggcgtc tattccattc
      781 cgcctgatat tagcggcgtc attggtgaag tagatttgag cggcgcgtta acacttgaac
      841 tcgccggtat cattttctcc tttatgctga tcaacctatt tgattcatca ggaacattaa
      901 ttggtgtaac tgataaagct ggcttaatag atagtaacgg taaattcccc aatatgaata
      961 aggcgctata tgttgatagc gtagttcggt ggcgggcgcg tttatcggca cctcgtctgt
     1021 taccgcctat attgaaagta cttctggtgt ggcagtcggt ggtcgcacgg ggctgactgc
     1081 ggttgtggtc ggcgttatgt tcctgttggt tatgttcttc tcaccgctgg tggcgatggt
     1141 tcctccttac gcaaccgccg gagcgttaat ctttgttggc gtgttgatga cctcaagtct
     1201 ggcgcgcgtt aactgggatg attttaccga atcggtgcct gcgtttgtta ccacggtgat
     1261 gatgcccttt actttctcga tcaccgaagg gattgcactt ggctttatgt cgtactgcat
     1321 catgaaagtg tgcaccgggc gctggcgcga tctgaacctg tgtgtggtgg tggtcgcagc
     1381 tctgtttgca ctgaagatta ttctggtgga ttagtaactg gcgagggttt ccagatatca
     1441 tgagttctga ttacgcagga gaactcatga tctggataat gctcgccacg ctggcggtag
     1501 tgtttgtggt tggttttcgg gtgctgacat ccggggccag aaaagcgatt cgccgtctca
     1561 gcgatcggct gaatatcgat gtcgtacccg tggagtcgat ggtcgatcaa atgggaaagt
     1621 cagccggtga cgaattttta cgttatttgc atcgtccgga tgagtcgcac ctgcaaaacg
     1681 ccgcgcaggt gttgctcatc tggcaaattg tcattgtcga tggtagcgaa cagaacctgc
     1741 tgcaatggca tcggatttta caaaaagccc gccttgccgc tcccattact gatgctcagg
     1801 tcaggctggc gctgggtttt ctgcgcgaaa ccgaacctga aatgcaggat attaatgctt
     1861 ttcagatgcg ctataacgcg ttctttcagc ctgccgaggg cgttcactgg ctgcattaaa
     1921 accgcttcgt cttatcagcc tacagaccgc gcctgacctg tagggtggat aaggcgttca
     1981 cgccgcatcc ggtatcggaa ctgacagtag gtgaacgcat tatctacgtt ctatttacag
     2041 cagcacctcc aacccgtgtt tttgcacaat gctgagcagt tttaatgaaa tgccattggg
     2101 gcgtttagcg ccactttccc atttttgtac agttgaaacg ctggtattga gatagtgagc
     2161 gaagacgggc tgactcactt ttaatttttc ccgcagtgct tttatctcaa caggttgtaa
     2221 gtcttgcacg ctggtcaggc aggctttgtc aaaagtacgc atcgtttcag cagaaatagc
     2281 attgacgctg gacaatccag ccgctgcgct gtgaatagcc tcaaatgcag gacttttaaa
     2341 tttagttttt gcagtcatag cagatctctg tcagttcttt agtgttaatc attgctgtta
     2401 gttgggcttt cgtcaggaag gcataatgct ttgcaatttc acgaaaccca gccagttcct
     2461 gactattgat gttcgacata tcctgttttg catataaaaa cgtgtaaaac caattgctta
     2521 cccctttcgc cagaataatt gcacgctcac gattttggtg aaggcgcttt ttaaaaacgc
     2581 caccgccaag tgcatcagct ttaccttgtt ctgtttctaa aatggcccgg cacaactctt
     2641 catctgttat tgcatgagac ctggcttccc ttgcaaacca tttggtttta aacacgcgta
     2701 tatcgttgag gtcctgttat taaagtatat caccaagtgc aatactttat cccttgttcc
     2761 caacacaatc gcgaaaatac atatcacctt gtcctatgat ttttatttct tcgagcagcc
     2821 tcgagctttt cccacaacac cgtaaaagtt gcaaaaacgt taaatgcgtc acacatttca
     2881 atgttaaagt ggtcggcttt tcccctgaaa catgccacgg gtaacaccat gagtgaattt
     2941 attgccgaaa accgcggcgc ggatgccatc acccgaccga actggtcagc cgttttctcg
     3001 gtggcgtttt gcgtcgcctg tctgattatc gttgagtttt tgcctgtcag tttgttgacg
     3061 ccaatggccc aggatttagg catttcggaa ggggttgccg ggcaatcggt gaccgtgacc
     3121 gcctttgtgg caatgtttgc cagtttgttt attacccaga caattcaggc cactgaccgc
     3181 cgctacgttg ttattttgtt tgccgttttg ctgacgctct cctgcttgct ggtttccttt
     3241 gctaactcat tcagtttgct tttaatcggt cgtgcctgtc tggggctggc gctgggcggg
     3301 ttctgggcga tgtcggcgtc gctgaccatg cgtctggtgc cgccgcgtac ggtgccgaag
     3361 gcgctgtcgg tgatcttcgg cgcggtttct attgcgctgg tgattgccgc gccgttgggc
     3421 tgttttttag gcgagcttat cggttggcgc aatgtcttta atgcggcggc ggcgatgggt
     3481 gtgctgtgta ttttctggat tatcaaatca ctgccttcgc tgccaggtga accttcgcat
     3541 cagaaacaaa atactttccg cttattacaa cgtccgggag tgatggccgg gatgatcgcc
     3601 atctttatgt cctttgccgg acagtttgct tttttcactt atattcgccc agtgtatatg
     3661 actctggcgg gattcggtgt tgatggctta accctggtgc tgctgagttt tggtatcgcc
     3721 agctttgtcg ggacatcgct ttcgtcgttt attctcaaac gttcggtgaa actggcctta
     3781 gcgggcgcac cgtttgtgct cgccctgagc gcgctggtac tgacgctgtg gggaagcgat
     3841 aaaattgttg ctactggtgt ggcgattatc tgggggctga cctttgcttt aattcccgtt
     3901 ggttggtcaa cgtggatcac tcgctcattg gccgatcagg cagaaaaggc tggatctatt
     3961 caggttgccg ttattcagct tgccaatacc tgcggtgcgg caatcggcgg ttatgcgttg
     4021 gataacattg gtctgacttc gccgctgatg ttgtccggca cattgatgtt gctgactgca
     4081 ttgttggtga ctgcaaaggt gaaaatgaag aaatcctgat atcaggtaaa gaagcgttgg
     4141 tcagtggttg ttgccgttct tacatatccg ggcattctac cttacccaac gcttcccaat
     4201 aattgctctg attattacgt tccatcgcca cgatggcctc gcgtatatgt tgtttattgt
     4261 tttcatctga caacattaac ttttcccacg cttcatctga taatgacgcc tgtggcgtgc
     4321 aaattttacg taaatcttgc aataccgtgg ttttctcttt tgccgactgt agagaactga
     4381 atggcgactc ggcctgagaa agggctggaa aaatcaggaa tacggtaatc atgagtagca
     4441 tcgttggctt catagcggca accttctgca aatgtgcatc gaatgaagca taagtgtagt
     4501 ttgcttttgc cgctctcatc atgctgtacg aaaggtgttt gcgtggtcag taagaagtgc
     4561 cagactttat attccacttt tattcctttt tattcttatc gatagcgttt tgttttttaa
     4621 actgcagcga ccttaccgct atagtccggt aatcattaat aaaaggataa aaaaatgaaa
     4681 ctgacaacac atcatctacg ggcgggggcc gcattattac tggcaggagt tctgctggca
     4741 ggttgcgacc agagcagcag cgatgaaaag catattaaag ttggcgttat taatggcgca
     4801 gaacaagatg tcgcagaagt cgctaaaaag gtagcaaaag agaaatatgg cctcgatgtt
     4861 gaactggtag gatttagcgg ttcgttgcta ccgaacgatg cgacaaataa tggcgagctt
     4921 gatgcgaacg tcttccagca tcgacccttt cttgaacaag ataatcagac gcatggttat
     4981 aaactggtag cggtgggaaa tacgtttgtc ttccctatgg cgggctattc caaaaaaatc
     5041 aaaaccgtgg cgcaaataaa agaaggtgcg acagtggcga tccccaacga cccgactaac
     5101 cttggtcgcg cgcttttact gctgcaaaaa gagaagctaa ttactctgaa agagggaaaa
     5161 gggttattac ctaccgcgtt ggatataacc gataatccac gccatttgca gataatggag
     5221 ctggaagggg cacagttgcc acgtgtactg gacgacccga aagtggatgt agcgattatc
     5281 agcaccactt acatcgagca aacaggactt tctccggtta acgacagcgt atttattgaa
     5341 gataaaaatt cgccgtatgt gaatattctg gtggcacggg aagataataa gaatgcagaa
     5401 aacgtgaagg aatttctgca atcttatcaa tcacccgaag tcgctaaagc ggcagaaacc
     5461 atctttaacg gtggtgcggt gcctggctgg taattctcac tcatgccgcg ccggacgatt
     5521 tatttttctg gcgcggcggc gggaatccat tgaaatcaaa atcaccgatg acagaatcag
     5581 cagcgttccc agccagtccg gcaatgtgaa ggtgatcccc agcaacagca aagagagcag
     5641 cgcgctactt aatggttcag cacagcttaa aatgctggct tttggaccgc caattaattg
     5701 tgctcctttc aggtacagac taaatgtcag ggacgtacca atgaccacca aataaaaaaa
     5761 cgccagaatc aaacttccgt taacgataaa gtttgtccct tgtccggcat aaaagggcag
     5821 caggatcaga ccgccaatga gcatactcca gccgacgacc ggtaacgtgc catagcgggc
     5881 aatcagcgtt gagggatagg tggtatagaa tgcagcagta aatgccgagg caatgcccca
     5941 gaataacgcg gcaggagaga tcgataatga cgtcggatta ccgtgtgtca ccaataaaaa
     6001 agtcccgatc agcgatgtca aaatagcgca gaaaaccaga atgcccgggc gggatttacg
     6061 caccagtgag aaccaggcga cgataatagt cggtgagagg aattgcagca ccgttgccgt
     6121 ggctgcgttc gatttttcga tggttagcaa aaaagtgagc tgtacggtta gcgcgccaac
     6181 cacggaaaaa atcagtaggc taatggcatc tttgtgattg ttaataatag aaaagatttt
     6241 atcaccatga acaaatgata gcgtcagcag aatcaaaccg gcgaatatca aacgcgtcat
     6301 agtcaaaaac tgcgacgaca tctggctttg ctccatgatg tattgcgcgc agacccctga
     6361 acttccccac aacacggcgg caatcagaac gttcagcatc ccctttctgg tggaacccat
     6421 ttttccctct gcatactgtt ttgtcgttta tgtatgcagg catgatagca aaaatggcga
     6481 ggatggctca gtttgcgcca cgccgatttt tcttagccca gactggtgag ctaaatatct
     6541 ttaataaaca gcaagcaaat cattgcaata cccaacatcc ctatcgccag ccagaacaac
     6601 gtgtggtagc tccagatttc aaccataatt tcgtcaacag agccggcgat aatctagccg
     6661 aagcgtgagg tatttgcata taacgttgtt gcggaaccta ttcttattta tcggagtgcc
     6721 ttgccagtaa tttactgacg aggatcctca taatagcgtt acccatctgt tgcattgaga
     6781 ctttacaggc cgttattcta aatgaccaaa attaaatcag tgcataaggg gaagtattct
     6841 gtagtgatgt ctccctgaga tgcaggttcc actggagaaa attatgagca gtttgtttca
     6901 gaatgaaata aaggataaat ttaaaactgg acttgcatat gtgcgggggc cagaaaaaag
     6961 cggatcttcc gctcaaaatg caggtagcga aggtggtaaa aaatgcgccg ggagaagcag
     7021 aagattgtcc cgcgatgtcc gtttgagaaa cacgagtttg attaaacgcg tgagcatgat
     7081 gatattagta gctgaccaga gaaattatat ccgtcgtaag caataaaaat cagaagtgat
     7141 gttatggata tttttaacct gaataactcc gtaagtacgg ggttattcag gtttttgcta
     7201 gcctgtgctt ttctatacca gatgtggtgt gaacactcta ttctgcatac aaattaaata
     7261 tattgtagga tctaatataa gtctgataat actaatagac tgagtaagat tctggttgat
     7321 aataactgat atccttttca actattaatg tgtttattta gcgtgaaatt atactcgtat
     7381 ttttataact ttatgagatt gccaggtttg gtcgataaat aataaggcca gtgacagact
     7441 tacttattag ggacaaattt cgcgaggata tcaggttcac tggcaacata aatgctttca
     7501 tcaggcagag ttacaattcc ttcaggttgg ggcaaatcac tggtcagaga atgatttcct
     7561 tttgtcaggt caagcatctc aatgaaagac atctctgtca ggttaaactt gagtaattta
     7621 cgcgattcat cggacagaat catcagactt tcattattaa aggccaggcc ggatatatcg
     7681 ttaacacgta tatcatttag tgcctctgga atagttgctt gctttacact cagcaggtct
     7741 ggagaaagtt gatatataaa tattttactt gggcgtcttt cttttgcgac aaacagcatc
     7801 cgatcatctg ctttccatgc cagcccttca aaccctctat tctttttatt gaatttttct
     7861 aactgaattt tatttcgaaa ggagatatgc atatcttttg taactgtaaa aaagaatagt
     7921 tcagaagtct tttcgtctac tgcagcaaaa atgttccctt gtatgtgttc aattgtttcg
     7981 gcatcagaga taaaaggcag tggggctctg tccatgatgt cgccactttt ggttatttta
     8041 agtagctgtg cagggctatt ctgggtggca tacaggaaat catcctcttt attataagta
     8101 agagatgata agttcaccgt atttggtaat tgttttataa atgaaagtga gtgttcttga
     8161 ggtgattttt tgtaagtaca attaaatgga aataatatta atatggttag tgcaataaat
     8221 atcgcgatga aaaatgcaat caatggtttt cttttcattt tttctatttg ctgtaaattg
     8281 tttgcagaat aaatatcttt gtttaattgt gattaacttc ggaataaatt gttaataatt
     8341 atgggtgtgg tttctattat tagttttgat taataaagtt ttattttaag tgttttgtaa
     8401 gtacgtttca gatgcggcat accttggttg tagcgcactt accatttcat cgattataca
     8461 tttcacacct tgcggcactt cataagaagt cctgttcgtc aacatgccca tctcgttggg
     8521 gcggtcttct ggcgccatta tcagaacccc agtatggctg gtaacataaa tgtttccatc
     8581 tgatgattga gagatactga tgttaatgca agcgttaggt gtatctaatg atctttccgc
     8641 attgtattca ctggctttag agatcctttc atgtgtcaac gttaaggctg gcacattgaa
     8701 agattgatag aaagccgagc atatggccgt tgctgcttct ggatggcata aaaattccac
     8761 aacttcagct ttctgtttat cactgccgct gctttctctt gctatttgct ctgcatcatg
     8821 gcgagaaata ccatgatcta taacattccg ggttggtgat gaatgtgtgt ctttaagtcc
     8881 tagtgcatcg cgaagcaatt tttgtgcatt tgcaggtata tcaacaccat tgagtgaaag
     8941 ctttgcattg ccgagtcctc gacatgtatc cataaagata gagctgaatg gtgtaggccc
     9001 ggaaagcgga tctgtttgag cattatgagg aatcaaggtt gttgatagtt ttgacgtaaa
     9061 accaagtaga ttgcgcattg aggtagtatc agtcaggtcc atctcagata aggcacgatg
     9121 aacctgaatg tatggcatgt ggcgatcgat gggtaaagat tgtaataacc tattttgttc
     9181 gtttgactcc aggagcacag taatcttttc attatttgct gtgcgaatct cggcttcaac
     9241 aagttcgctg ttagtgacgg aaattcttaa ggatgaacca tctgaaaaac gagcagatac
     9301 tctgcctaat tcattgggaa gtggatatac aggctctaaa acggttaaca actcttgaaa
     9361 tgattgtctt tcactacgac tgtacgttgg attccataaa ccaaaactaa gtactttaaa
     9421 taacttttca gcgcatgaca tctcatccca gcttttttta aacccagaat taactttcat
     9481 tgcagcatct aaaactaaag atgcggagtc attattaagt ccatttatca tgagagattc
     9541 tcctatctgg tttgtaatga acaatttggc aacaa
//
LOCUS       DAZFBR010000150         9561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFBR010000150 DAZFBR010000000
VERSION     DAZFBR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.150"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            289..2526
                     /gene="nrfB"
                     /locus_tag="ACHJZ2_004325"
     CDS             289..2526
                     /gene="nrfB"
                     /locus_tag="ACHJZ2_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO4570318.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2513..5485
                     /gene="nfrA"
                     /locus_tag="ACHJZ2_004326"
                     /pseudo
     CDS             2513..5485
                     /gene="nfrA"
                     /locus_tag="ACHJZ2_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            5486..6376
                     /locus_tag="ACHJZ2_004327"
     CDS             5486..6376
                     /locus_tag="ACHJZ2_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO4570319.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6559..7320
                     /gene="envY"
                     /locus_tag="ACHJZ2_004328"
     CDS             6559..7320
                     /gene="envY"
                     /locus_tag="ACHJZ2_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO4570320.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7366..7442)
                     /locus_tag="ACHJZ2_004329"
     tRNA            complement(7366..7442)
                     /locus_tag="ACHJZ2_004329"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7406..7408),aa:Arg,seq:tct)
     gene            7685..8317
                     /gene="fimZ"
                     /locus_tag="ACHJZ2_004330"
     CDS             7685..8317
                     /gene="fimZ"
                     /locus_tag="ACHJZ2_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO4570321.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(8320..8835)
                     /gene="sfmF"
                     /locus_tag="ACHJZ2_004331"
     CDS             complement(8320..8835)
                     /gene="sfmF"
                     /locus_tag="ACHJZ2_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO4570322.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(8846..>9561)
                     /gene="fimH"
                     /locus_tag="ACHJZ2_004332"
     CDS             complement(8846..>9561)
                     /gene="fimH"
                     /locus_tag="ACHJZ2_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO4570323.1"
                     /translation="QLNDYLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVM
                     DSRLIFRLKVIRPFINMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCE
                     LNAGQIVEFDFGDIGASLFSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEAS
                     AVSGQAMVSDNQDLGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITG
                     QKPTEGPFSALGYLRVDYQ"
BASE COUNT         2429 a         2464 c         2380 g         2288 t
ORIGIN      
        1 ttgcggggag cgggctttcg taatcttgcc ctgcctgctc acctccaggc acttccacta
       61 catcatgacc tgatcggtgc caccactcct cttcactatg acgttaacct cccctccttg
      121 acaccacgct tttgtctaca ttctacgcaa acggcaactt ttataaccag tgtaaaaata
      181 acgtgccgca atagtcatac ctattaatgg taaaaagctg tcacaattca taaaaaacct
      241 taatatacgc caccctaaac ataaccagcg ttaatgtaag gtttttgtgt ggactggctt
      301 cttgatgttt ttgctacctg gctctatggc ttaaaagtaa tcgcgataac gttagcggtc
      361 atcatgttca tcagcgggct ggacgatttt tttattgatg tcgtctactg ggtacgccgc
      421 attaaacgca agttgagtgt ttatcgccgc tacccgcgaa tgagttaccg cgaactgtat
      481 aaaccagatg aaaaaccgtt agcgattatg gttccggcgt ggaatgaaac gggcgtcatc
      541 ggcaatatgg ccgagctggc ggcgaccacg ctcgactacg aaaactatca tatctttgtt
      601 ggcacctacc ccaacgaccc ggacactcag cgtgatgttg acgaagtgtg cgctcgcttc
      661 ccgaatgtgc ataaggtagt ctgcgcgcgt cctggcccca ccagcaaagc cgactgtctg
      721 aacaacgtgc tggacgccat cacccagttt gagcgtagcg ccaatttcgc ttttgctggt
      781 tttattctgc atgacgccga agatgtgatt tcaccgatgg aattgcgtct gttcaactat
      841 ctggtcgagc gtaaagatct gattcagatc ccggtgtatc cgtttgaacg cgaatggacg
      901 cacttcacca gcatgactta cattgatgag ttttcagagc tgcatggcaa agatgttccg
      961 gtgcgtgaag ccctcgccgg acaagtgccc agcgcaggcg tcggcacctg tttcagccgc
     1021 cgcgccgtga ccgctctgtt agctgacggt gacggtattg ctttcgacgt gcagagtctg
     1081 actgaagatt acgacattgg cttccgcctg aaagaaaaag gtatgacgga aatttttgtc
     1141 cgttttccgg tggtggacga agccaaagaa cgcgagcagc gtaaattttt acagcacgca
     1201 cggacgtcaa acatgatctg cgtgcgcgaa tatttccccg ataccttttc gactgcggtt
     1261 cgacaaaaat ctcgctggat catcggcatt gttttccagg gctttaaaac ccataaatgg
     1321 acctccagcc tgacgctgaa ctactttctc tggcgcgacc gcaaaggggc aatcagtaac
     1381 tttgtcagct tcctcgcaat gctggtgatg atccagcttt tgctgttgct ggcgtatgaa
     1441 agtttgtggc ccgatgcctg gcatttcctt tctattttta gcggcagcgc atggttaatg
     1501 accctgctgt ggctaaactt tggcttgatg gttaaccgca tcgtgcagcg ggtgattttc
     1561 gtcactggct actacggcct gacgcagggg ctactatctg tcctgcgtct tttctggggc
     1621 aacctgatta actttatggc caactggcgc gcgttaaaac aggtacttca acacggcgat
     1681 ccacgtcgcg tcgcgtggga taaaacaacg catgacttcc ccagcgtcac tggcgatacc
     1741 cgctcgttgc gcccgttagg tcaaattctg ctggaaaatc aggtcatcac tgaagaacag
     1801 ctcgatacag cactgcgtaa tcgcgtcgaa ggtctacgcc tgggcggttc aatgctgatg
     1861 caggggctga ttagcgccga gcagctggca caggcgctgg cagagcaaaa cggcgtggcg
     1921 tgggaatcca tcgatgcctg gcagatcccg tcctcgctga ttgccgaaat gccggcctcc
     1981 gtggcgctgc attatgcggt actgccgctg cgtctggaaa atgatgagtt aattgtcggc
     2041 agtgaagatg gtattgaccc ggtttcgctg gcggccctga cgcgtaaagt cggacgcaaa
     2101 gtgcgttatg tcattgttct gcggggacaa attgtcacgg ggttacgtca ctggtatgca
     2161 cgccgacgcg gtcacgatcc gcgggcaatg ttgtacaatg cggttcagca tcagtggctc
     2221 acggaacagc aggccggtga aatctggcgg caatatgtgc cgcatcagtt cctgttcgcc
     2281 gaaatactga ccacgctcgg tcatattaat cgttcagcaa ttaacgtgtt gttattgcgc
     2341 catgaacgca gttctctgcc gctcggcaag tttttggtca ccgaaggcgt tatcagccag
     2401 gaaacgttgg atcgcgtcct gacaattcaa cgcgaattac aagtttcgat gcaatcacta
     2461 ttactcaaag caggtttaaa cacagaacag gttgcgcaac tggagtccga aaatgaagga
     2521 gaataacctt aatcgcgtca tcggatggtc tggtttactg ctgacgtctt tattgagtac
     2581 cagcgcactc gcagacaata tcggcaccag cgcagaagag ctggggctga gcgattatcg
     2641 ccattttgtt atttatcccc gtctcgataa ggcgctgaag gcacagaaaa ataacgacga
     2701 agcaaccgcc atccgcgaat ttgaatatat acaccagcag gtgccggata atattccgct
     2761 gactttatac cttgcggaag cctatcgcca ttttggtcat gatgaccggg cacggctgtt
     2821 gcttgaggat caactgaaac gtcacccagg agatgcccga cttgagcgca gtctggcggc
     2881 tattccggtt gaagtgaaaa gcattacaac tgttgaagaa ctgcttgccc agcaaaaagc
     2941 gtgcgatgct gcgccgaccc tgcgttgtcg cagtgaagtc gggcagaatg ccctgcggct
     3001 ggcacagtta cctgtcgcca gagcgcaact gaacgacgcg acgtttgctg catcgccgga
     3061 aggaaaaacg ctgcgaaccg atctgctgca acgggcaatc tacctgaaac aatggtccca
     3121 ggcagatacg ctatacaatg aagcacgcca gcagaacaca ttaagcgcgg cagaacgccg
     3181 tcagtggttt gacgtgcttc ttgccgggca gctggacgat cggatcctgg cactgcaatc
     3241 acaggggatc ttcaccgatc ctcagtcata tattacttac gcgagcgcgc tggcttatcg
     3301 tggcgaaaaa gcacgcctcc agcattatct cattgaaaat aagccgctgt ttaccacgga
     3361 cgcacaagag aaaagttggc tctatctgtt atctaaatac agcgccaacc ccgttcaggc
     3421 attggcgaat tatacggtgc agtttgccga taaccgccag tatgttgttg gcgcgacgct
     3481 accggtgctg ttaaaagaag gtcagtacga cgcagcgcaa aaactgctcg ccaccctccc
     3541 cgccaatgaa atgcttgagg agcgttatgc tgtcagcgtg gcgacccgta acaaggctga
     3601 agctctgcgt ctggcacgct tgctgtatca gcaagaatcg gcaaatctta cccgcctgga
     3661 tcaactaacc tggcaactga tgcagaacga gcagtcacgg gaagctgccg atttattgct
     3721 gcaacgctat cctttccagg gcgatgcgcg tatcagccag actttaatgg cgcgactggc
     3781 gtctctgctg gaaagtcatc cttacctggc aacgccggcg aaggtggcga ttttatcgaa
     3841 acccttaccg ctggcggagc aacgtcagtg gcaaagtcag ttgccgggta ttgcagataa
     3901 ttgcccggca atagttcgct tgctgggcga tatgtcgcct tcctacgatg ccgccgcctg
     3961 gaaccgtctg gcaaagtgtt atcgggacac gctacccggt gtggcgttgt atgcatggct
     4021 tcaggccgaa caacgacaac cgaacgcctg gcaacatcgt gcggtagcct atcaggcgta
     4081 tcaggttgag gactacgcca ccgcactggc ggccaggcag aaaatcagtc ttcacgacat
     4141 gagcaatgag gatctgcttg ctgctgccaa taccgcccag gcggcaggaa atggtgcggc
     4201 tcgcgatcgc tggctacaac aggcagaaca acgtggactg ggaagcaatg ccctctactg
     4261 gtggctgcat gcgcaacgtt acattcctgg tcagccggaa ctcgcactga acgatctcac
     4321 gcgctcaatc aatattgcgc cttctgccaa cgcttacgtt gcgcgggcga caatttatcg
     4381 ccaacgtcat aatgtcccgg ccgcggtgag tgatttgcgc gccgcgctgg aactggaacc
     4441 gaataatagc aacacccagg cagcgcttgg ttacgccttg taggatagcg gtgatatcgc
     4501 acagtcgcgg gaaatgctcg aacaggcgca taaagggcta ccggacgatc cggcactgat
     4561 ccgacaactg gcctatgtga accagcgtct ggatgacatg cctgcgacgc agcactacgc
     4621 ccggctggtg attgatgaca ttgataatca ggcgctgata accccactga ccccagagca
     4681 aaatcagcaa cgcttcaatt tccgccgtct gcatgaggag gtcggtcgcc gctggacgtt
     4741 cagtttcgat tcttccatcg gcttgcgttc cggggcaatg agtaccgcta acaataatgt
     4801 tggcggcgca gcgccaggga aaagctatcg tagctacggg caactggaag ccgaataccg
     4861 catcggacgc aatatgctgc tggaaggcga cctgctctca gtttatagcc gcttctttgc
     4921 cgataccgga gaaaacgggg tgatgatgcc ggtgaaaaat ccgatgtccg gcaccggtct
     4981 gcgctggaag ccgctgcgcg atcagatctt tttcctcgcc gtcgaacagc agttgccgct
     5041 gaacgggcaa aatggcgcat ccgataccat gctgcgcgcc agcgcctcat tctttaatgg
     5101 cggcaaatac agcgacgaat ggcacccgaa cggttcaggc tggtttgccc aaaacctgta
     5161 cctcgatgcg gcgcaatata tccgccagga tattcaggcg tggacggcag attatcgcgt
     5221 cagctggcat cagaaggtag ctaacggaca gactattgag ccttacgctc acgttcagga
     5281 caacggctat cgtgataaag gcactcaggg cgcgcagctt ggcggtgtcg gggtccgctg
     5341 gaatatctgg accggcgaga cgcactacga cgcctggccg cacaaagtca gtctcggcgt
     5401 cgagtatcaa cataccttta aggcgattaa tcaacgtaac ggagagcgca acaacgcgtt
     5461 tctcaccatt ggagtgcact ggtaaatgcg taagttcatt ttcgtattgc tgacactgct
     5521 tttggtcagc cctttttcct ttgcgatgaa aggtattatc tggcaaccac aaaaccgtga
     5581 tagtcaggtt accgataccc agtggcaggg gctgatgagt cagttacgtt tgcaaggctt
     5641 cgataccctg gttttgcaat ggacccgtta cggcgatgca ttcacccagc cagaacagcg
     5701 cgcgttattg tttaagcggg ccgcagctgc gcaacaggct ggcctgaagc tgattgtcgg
     5761 gctgaacgcc gatccggaat tttttatgca ccagaaacag tcatccgcgg cgctggaaag
     5821 ctatcttaat cgcctgctgg ctgccgatct ccagcaagcc agattatgga gtgccgtacc
     5881 tggcgtaacg ccggatggct ggtacttcag cgctgaaatt gacgacctga actggcgcag
     5941 cgaagccgcc cgtcagcctt tgctaacatg gttaaacaac gcgcagcggc tgattagcga
     6001 tgtttcagca aaaccggttt atatcagtag ttttttcgcc ggaaacatgt cgccagatgg
     6061 ctatcgccaa ctgctggaac acgttaaagc aaccggcgtt aatgtctggg tacaggatgg
     6121 cagcggcgtg gataaactta ccgctgaaca gcgtgaacgt tatctacagg ccagcgccga
     6181 ttgccaaagc tccgccccag caagcggcat tgtttatgaa ctgtttgtcg ccggcaaagg
     6241 caaaaccttt acagcgaaac cgaaaccgga cgcagaaatt gcctcgctgt tagcgaaacg
     6301 ttcctcttgc ggtaaagaca ctctctattt ctctctgcgc tatttgcccg tcgcgcacgg
     6361 cattctcgag tattaaatct cctccaggta agtcgggtac gacctggctt acctctttcg
     6421 ctctcgtcag aattccatca agattaattg ctaaaaagcg tgctaaagaa agaagatcgt
     6481 tatcggcgaa cgggccgatc actcatgcag gaagcataga tcctttgagc ggagtttctg
     6541 atatctgagg agtgcgaaat gcaattgagc agcagtgaac cttgcgtggt gatcctgacc
     6601 gaaaaagagg tagaggtaag cgtcaataac catgctacgt ttacccttcc gaaaaactac
     6661 ctggccgcct tcgcgtgcaa caataacgtc attgaactct caacgttaaa tcacgtatta
     6721 atcacccaca tcaaccgtaa catcatcaac gattatctgt tgtttttaaa taagaactta
     6781 acctgtgtaa agccctggtc gcggctggct accccggtta tcgcttgtca tagccgtaca
     6841 ccggaagtgt tccggctagc cgccaaccac agcaagcagc aacccagcaa accctgcgaa
     6901 gcggagttga cgcgcgcatt gctttttacc gtattgtcta actttcttga gcaatcgcgg
     6961 tttattgccc tactgatgta tatcttacgc agcagcgtcc gcgacagcgt ctgccgcatt
     7021 attcaaagcg atattcagca ttactggaat ctgcgaattg tcgccagttc gctatgttta
     7081 agccccagcc tgctcaaaaa gaaattaaaa aacgaaaata ccagctatag ccagattgtc
     7141 acagagtgtc gtatgcgtta cgccgtacag atgttattga tggataacaa aaatatcact
     7201 caggtggcgc aattatgtgg ctatagcagc acgtcgtact ttatctctgt ttttaaggcg
     7261 ttttacggcc tgacaccgtt gaattatctc gccaaacagc gacaaaaagt gatgtggtga
     7321 agggcaaagc ggaaacggat aagacgggca taaataaaga agaaatggcg cgccctgcag
     7381 gattcgaacc tgcggcccac gacttagaag gtcgttgctc tatccaactg agctaagggc
     7441 gcgttgatac cgcaatgcgg tgtaatcgcg tgaattatac ggtcaaccct tgctgagtca
     7501 atggcttttg atctggttgc tgaacaagtg aacgaccgcg tctgattttc tgatttattt
     7561 cgctatagcg gcaaacaaac gcacaccgct gcgcgtctga atcaagaaaa cccgtatttt
     7621 catgtatcaa agtacaattt accgccctaa cggaaaattg tccgctccta tgagactggt
     7681 aactatgaaa ccaacgtcgg tgatcattat ggatactcat cctatcatca gaatgtctat
     7741 tgaagttctg ttgcaaaaaa acagtgaatt gcagattgtc ctgaaaacgg atgattaccg
     7801 cataaccatc gattatctcc gaacccgtcc tgttgattta atcattatgg atatagactt
     7861 gcccggaaca gacggtttta ccttcctgaa aaggatcaaa caaatccaga gcacagtgaa
     7921 agtgttattt ttatcatcga aatcagaatg cttttatgct ggcagagcga tacaagctgg
     7981 tgctaacggt tttgtcagta aatgcaatga tcagaatgat atttttcatg ccgttcagat
     8041 gatcctctcc ggatacacgt tttttcccag cgaaacgctt aactatataa aaagcaataa
     8101 atgtagtacg aatagttcaa cggtcactgt gctatctaat cgtgaagtga ccatattacg
     8161 ttatctagtt agcggattat ctaataaaga aattgccgat aagttattac ttagcaataa
     8221 aacagttagt gcgcataaat ctaatattta tggcaagcta ggtttgcatt caattgtaga
     8281 gcttatcgac tacgccaaat tatacgaatt aatataatat taattataat tgatcataaa
     8341 tatcgcatcc gctttcgcca cacctggccg aacaccgctg gctaacgctc gataattggc
     8401 aaaaaagttt agtgtgacat tgccatttgc atctacttcc tcagtcgggc tcgcctcccc
     8461 cagtgcgagc cgggagcgat cgctattacg taattcgatg gcgacggttt gtgccattgc
     8521 gggatcatcc agagccagca ggttggcatc agatgccggc gttcccgtaa ataaaatcgc
     8581 aactgaaccc ggaggacatc cctccagccg caggctaaaa gggaggagtg ccgtggtatc
     8641 gccagcgttc agtaattgtg tcgtaggcca tctgcctaaa tctaccgtct tatcaatatc
     8701 cgctgtgttt acggtacagg agaaatcaac aacgttaccg tgcaaattga tattaatcgt
     8761 tcctaaaggg tcagctgccc atccactgga actccacagt aacccgcaga aacagctaaa
     8821 gaatactctt ctcattctcc gtacctcatt gataatcgac gcgtaaatac cccagcgcgc
     8881 taaacggccc ttcggtcggt ttttgaccgg taatactgat aggccaggcg cgaagtgtga
     8941 cattggctgc cgcagctgca tccagacgga aaggaataac gctattgaga tcgttaggcg
     9001 tgatcggcgt atcgttctga tcggcgacaa taaaacctaa atcctgattg tccgacacca
     9061 tcgcctgacc agaaacggca ctggcttcca gacgcattgt taaataagcc tgcgcagcaa
     9121 cattcgtaca tttgaccgca atgctcttgg tttgcggcat gacaccagca ggccgattac
     9181 ccggccctgc cgcactaaat aacgatgcgc cgatatcacc aaaatcaaat tcaacaatct
     9241 gcccggcatt taattcgcag ttttgcggta cttcaacccg gccaccaaaa ctaatggtat
     9301 aaacaggtgt aaccaacgga tcgtaaggcg ttgatgtcac atacacggta aacatcacct
     9361 gtctggggat ctccaccatg ttaataaagg gccgaataac ctttaaacga aatatcaaac
     9421 gagaatccat caccccgaac ggaaggtttt gcgaaacgtt aggatctgtt cccatacgaa
     9481 tataattttg cgggggaaat tgaatatcca tcacactatc aaccagactc atcgcaccta
     9541 atagataatc atttaattgc a
//
LOCUS       DAZFBR010000151         9549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFBR010000151 DAZFBR010000000
VERSION     DAZFBR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.151"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..783)
                     /locus_tag="ACHJZ2_004333"
     CDS             complement(<1..783)
                     /locus_tag="ACHJZ2_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4570324.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTL"
     gene            complement(1026..1217)
                     /locus_tag="ACHJZ2_004334"
     CDS             complement(1026..1217)
                     /locus_tag="ACHJZ2_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO4570325.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            1235..1387
                     /locus_tag="ACHJZ2_004335"
     CDS             1235..1387
                     /locus_tag="ACHJZ2_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570326.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKSSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            1569..3812
                     /gene="pdeF"
                     /locus_tag="ACHJZ2_004336"
     CDS             1569..3812
                     /gene="pdeF"
                     /locus_tag="ACHJZ2_004336"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO4570327.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(3851..5392)
                     /gene="ppx"
                     /locus_tag="ACHJZ2_004337"
     CDS             complement(3851..5392)
                     /gene="ppx"
                     /locus_tag="ACHJZ2_004337"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO4570328.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(5397..7463)
                     /gene="ppk1"
                     /locus_tag="ACHJZ2_004338"
     CDS             complement(5397..7463)
                     /gene="ppk1"
                     /locus_tag="ACHJZ2_004338"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO4570329.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSHSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(7634..8272)
                     /gene="purN"
                     /locus_tag="ACHJZ2_004339"
     CDS             complement(7634..8272)
                     /gene="purN"
                     /locus_tag="ACHJZ2_004339"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO4570330.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHPHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(8272..9309)
                     /gene="purM"
                     /locus_tag="ACHJZ2_004340"
     CDS             complement(8272..9309)
                     /gene="purM"
                     /locus_tag="ACHJZ2_004340"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO4570331.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
BASE COUNT         2420 a         2288 c         2268 g         2573 t
ORIGIN      
        1 cagggttaaa cagcctgcca cttcatgttg gttatagttt ttattgcatg ccataacaat
       61 catccccggt gaggattttg caagagggaa gtataaccac ggatattttt tgcagttaat
      121 ccccgaatcc agtatccgtg ttttatattt atcgaactct ttcaacccat caattgtatc
      181 ttctgttaca ttaaaatggc agccattaat ttccgcaaca gtcttgtaat ccttgaaaaa
      241 tggcttcggt tgattattat tccatagcca atatgcagaa tatgcgccta tgacaaatgc
      301 gctcaacata attagataac tttttatatg tttgaggatt ttgttattga aattctttgc
      361 gccaccaatg ctctctttgt atcccgcatt aacttttatg gcaggtgggg cattgtgtgt
      421 atgtgcgtct gttgatgagt ttattacatg attttgtatg ttaatgtcat tatggatctt
      481 gaatcctctt ctcggcacgg ttgcaataag cgagtgggta gtatcaccaa cagcgcgaaa
      541 tccgcgtctg ataatggata tgttctgata taacgtgtta gtaggcacac gcataccttc
      601 ttcttcccag actctggtga aaaaatcttg ttgtgcaacc acatcaggaa aagcttcaag
      661 tagcagtgat aaacaacgac tggctggcgt tgtcagaacg acgttaagat cggcgttatg
      721 taccgatatt aattttcggt gctcaggcca gaactcgata ttatcgttaa taatccagta
      781 catacgtcaa taaacactaa atcaatcgaa atggaaatca catagtttgc taagtatatt
      841 ggtatttacg gcataaatat atattaattt tatatttatt tcgcagccaa acaggataaa
      901 catcagtcgg gacaaagacg tataatttaa gatgaaagaa tatctgccgc tttgataatt
      961 ccaaagagca aaagcatccg acagcgaagc gccgttatgt tctgtgaaag tggagaaggt
     1021 aaccgctaac gttgtaccgg tgattcgacg gagagtgtgg attgctgagc ttcttttttg
     1081 ctctgatggt gctgccaggc accgacggac gagtagataa aacggccaaa gaagaagata
     1141 aagctgatga gcagtacgat acgggtcatg cgactgttaa atcggtgtcg ttttcgcata
     1201 ctggttgcct gactcacaaa aggttccttg aagtatgtcc cacgccgtgg acggtactta
     1261 cattaaggca caacaggaca aaatggtcaa ttcttcgtta tgtaaaaaat cgtcagtgga
     1321 tacatatttt aatgttatgg aagttaattt aattatttac aatgatgatg taataatgat
     1381 gaattaacat gataaacgta ataattcatt aatcagggtt attttgtttg atatatatca
     1441 attggaactt tcatatgaca cttagaatca atgctctcta tagtgatgaa taatcatcat
     1501 tcgaagtcag gtgggatgcc tgtctgaata cacctccttc aggatgtggg ggattctgct
     1561 gagcatctat gaaactgaat gcaacttata taaaaatacg tgataaatgg tgggggcttc
     1621 cgctgttcct gccttcttta atcttgccca ttttcgccca cattaatact ttcgcgcata
     1681 tttcttccgg tgaggttttt ctcttttatc tgcctctggc actgatgatc agcatgatga
     1741 tgtttttcag ctgggcggca ttgccaggga tcgccttagg gatttttgtc cgcaaatatg
     1801 cagagctggg tttttacgaa acgctctcat taacggctaa ttttattatc attatcattc
     1861 tctgttgggg cggttacagg gtctttactc cccggcgtaa caacgtttca catggtgata
     1921 cccgtttaat ttcccagcgt atattctggc agattgtgtt tcctgcaacg ctgtttctga
     1981 tacttttcca gtttgctgca tttgtaggat tactggcgag cagagaaaat ctggttggcg
     2041 tcatgccttt taatctcggg accttaatca attatcaggc cttactggtg ggcaatctga
     2101 ttggtgtccc gctgtgctac ttcatcattc gggtagtgcg aaatccgttt tatttacgta
     2161 gttattattc gcaattaaaa cagcaggttg atgtcaaagt caccaaaaaa gagttcgcac
     2221 tctggctgct ggcattaggt gctttactgt tgctgttatg catgccgtta aatgaaaaaa
     2281 gcacaatttt tagcaccaat tataccttgt cattattgct gcccctgatg atgtggggag
     2341 cgatgcgcta tggttataag ctgatttcgc tgctctgggc ggtcgtgttg atgatcagca
     2401 tccacagcta tcaaaattac attcccattt atcctggcta taccacgcag ctgaccataa
     2461 cctcctccag ttatctggta ttctctttta ttgtcaatta tatggctgta ctggcaaccc
     2521 gtcagagaac ggtagtcaga cgcattcagc gccttgcgta tgtggacccg gtggttcatc
     2581 tgccaaatgt tcgcgccctg aatcgcgcgt tgcgtgatgc cccctggtct gcgctttgtt
     2641 atttacgcat ccctggcatg gaaatgctgg ttaaaaacta tggcatcatg ctgcggattc
     2701 aatacaagca aaaactttct cactggctgt cacccttgct ggaaccaggt gaagatgttt
     2761 atcagctttc gggtaacgat ctcgcgctgc gactgaatac agaatcgcac caggagcgca
     2821 ttaccgcact ggatagccat ctcaagcaat ttcgtttctt ttgggatggc atgccgatgc
     2881 aaccgcagat tggcgtcagt tactgctatg tgcgctcgcc agtgaatcat atctacctgc
     2941 tgctgggaga gctaaatacg gtcgccgaac tttccatcgt gaccaacgcc ccggaaaata
     3001 tgcagcgtcg cggggcaatg tatttgcaac gcgaattgaa agataaagtc gcgatgatga
     3061 atcgactaca gcaggcgctg gaacacaacc attttttcct gatggcccag ccgattaccg
     3121 gtatgcgtgg tgatgtttac catgaaattc ttctgcgcat gaaaggtgag aatgatgaac
     3181 taatcggccc cgatagcttc ttgccggtcg cgcacgaatt tggtttatcg tcgagtatcg
     3241 acatgtgggt cattgagcat acgctgcaat ttatggctga aaacagagcg aagatgcccg
     3301 ctcaccgttt tgctattaat ctgtctccaa cctcggtatg tcaggctcgt tttcctgttg
     3361 aagtcagtca gttgctggat aaatatcaga ttgaagcgtg gcaacttatt tttgaagtca
     3421 ccgaaagtaa tgctctgacc aatgttaagc aggcgcaaat caccttgcag catcttcagg
     3481 aattaggctg ccagattgcg attgatgatt tcggcaccgg ctacgccagc tatgcgcggc
     3541 ttaaaaatgt gaatgccgat ctgcttaaaa ttgacggcag ttttatccgc aatattgtgt
     3601 caaatagtct ggattatcag atagtggcat cgatttgcca cctggcgcga atgaagaaaa
     3661 tgcgggtagt ggcagagtac gttgaaaacg aagagatccg cgaggcggtg ctctctttgg
     3721 ggatcgatta tatgcagggt tatcttattg gtaagccgca accgttaatt gatacgctga
     3781 atgaaatcga acccattcgc gaaagtgcct gaataatgcg ggccgacatt tctcgtcggc
     3841 ccgcaaagca ttaagctgcg atttcaggtg tactttcttc ttcaattttc aaccgccagc
     3901 cagccacgcc ttcccagtat tcttgctcct tttccagatc aagcagtacc agcgcattct
     3961 gactaaacca gtcatgcggg aaacgcagtg tccagtgact gtcatcagta atcagcgtca
     4021 atgttggcgg tgtggtggtt gcctgacgct gattgttgag taatacgcca aggcgcaata
     4081 gctgaatcag tggcaggaac tgtttcttct taaacaaggt aaagcgcggc agatcgtcga
     4141 gtttaatcgc tttacggtga tagcgcacca gtgtcgccat catcagctgc tgttcctgat
     4201 taaaacccgg caagtcgctg ttttgtagaa tataagcgga gtgacgatgc aaaccgctgt
     4261 ggttgatatt caacccgacc tcatgcagca tggcggccca tcgcagtagc gcctccagtt
     4321 gcggatgcgc cagcttcggt tgctgttccc gccactgttc gtacatttgc atagtggtat
     4381 ccagcacccg tcgggcctgt tcgctgtcga tgtgatactg gttggcgagg ctgctggcgg
     4441 tgcgactacg cacatcctga tgacggaaac gtccttccat ttcatacagt acgccttcgc
     4501 gtaacgcccc gtcagaaagg cgcagttcac ggatggctaa agcatcaaac acaccgcata
     4561 aaatcgctag tccaggaacg aagactgttt tccgctcttc ggaaagacct ggtaaactca
     4621 gcgatgcgaa attacggtgc cgtaaaactt cttttaccag tttttccaga cgttccgggg
     4681 taattatccc gtctttctcg cccatttcca ttaacacttc atgggcggct tttatggtac
     4741 cggaagcgcc cattgccacg ttccagccct gaatacgaaa ttgccaggtt aaagtttcca
     4801 gtttttgtgc tgccgccatg cgagcgcgct gaaaattctc tttattgatg accccgccag
     4861 ggaaataaag ctgggcaaag ctgacacacc ccatccggcg gctttcaacg agaatcggtt
     4921 cgaaattttc accaatcacc agttctgtag aaccgccgcc aatatcaata accagtttgc
     4981 gacctttttc cggttgggta tgttccacgc ccataaaaat cagacgggct tcttcattac
     5041 cggaaataat ttcaatcggg tagggaatga ccttttccgc acgtttcaga aagtcagtgg
     5101 cgttcagcgc ctgacgcagc gtatgggtac caactataca gacgctggca ggagaaaacc
     5161 cttgtagccg ttcggcaaac agcgacagac agttcaaacc gcgcgtcatt gcctcttcac
     5221 tcaacatatt atctggcccc aggccgtccg ccagatgcac ccgctgtttc aggcggccaa
     5281 taatctgcat ggcaccatct accacacggg ctatgaccat gtgaaaactg tttgaaccaa
     5341 gatcgaccgc agcaaactcc tgcggacgag gggatttatc gtgtattggc atagggttat
     5401 tcaggttgtt cgagtgattt gatgtagtca taaatcgcca actgcgcccg tactttgcgg
     5461 cgattgccgc ggggaacgta gcgattactg agttctttat cgatataacg tgctttgacc
     5521 gtatcgctga acaatatgtc gatgatgtcc agcacccgct gcttcaggcg cggatcgagc
     5581 agcggcgtcg ccacttcaat acgataatca atattgcgcg tcatccagtc ggcggaagaa
     5641 aggtagacct ttttatcgcc gccattttca aaaatataaa cccggtcatg ttcaaggtaa
     5701 cggtcaacaa tactgatggc acgaatgttg tcgctaatgc cttccagatt ggggatcagc
     5761 gaacacattc cgcgaaccag cagattaacc ggtacgccgg agctggaggc cgcatacaga
     5821 cgatcaacca ggcctttatc gacaaggtta tttagcttca gggtgatacc actgggcagc
     5881 ccttgctgcg cgttggcgat ctcgcggtcc accatttcat acaacaggcg gcgggagttt
     5941 tgcggcgaca ccattaaata atcaaatgtc accggacggt atgggttttc aataaagtta
     6001 aatacccgcc gtacttcgtt ggtgatgcgc gcatcggcgg tcagcaacga atagtcagta
     6061 taaagacgcg cggttttttc gttaaagttc ccggtcccga tgtgtgcata acgcaccact
     6121 tcaccgtttt ctttacgtga aatcagaaac agtttggcgt gaattttcag ccccggcgca
     6181 gagaagataa cgtgcacgcc tgcttcggtc aggcgcttcg cccagtgaat gttggcttct
     6241 tcgtcaaaac gtgcctgtag ctcaaccacc acggtgactt tcttaccgtt gtgtgcggcg
     6301 tggatcatcg agtcgatgat gcgtgaatct tttgccacac ggtaaatgtt gattttaatc
     6361 gccagcacgc tcggatcgaa cgaggcctga cgcagcagtt ccagcacatg ctcaaaggtg
     6421 tgataaggat aatagagcaa cacatcgcgt tcgcgaatgg catcaaaacc attgcggaac
     6481 tgggctttat caaaccaaat atggcgtaaa cgcggcagtg gtttgttcac cagattggct
     6541 ttgccgacat tggggaaatt aataaagtct ttaaaattat gataacgacc gccggggacg
     6601 atggagtcgt agcgggaaat agtcagtttt tcgcgtaaca cttcaaccag cgcattgggc
     6661 atatcgcgct gataaacaaa acgcaccggc tcagcagtta aacgctgctt gagactggaa
     6721 gacatcaact ccatcaggct ggcttccatc tcatgcacta aatcgtattc ggcatcgcgg
     6781 gtcatcttca ttgaataggc attcagcgcg tcataatcaa agaagccttt gaaaatatca
     6841 tcaaggcagt aacgcagaat gttatccaga agaatcattg gcttgcgtcg acgcggcgct
     6901 tctggcggta aattcacaaa gcgcggcact ttatctgatg ggatctccag aagcgcgtaa
     6961 cggatggtat cgccacggat aatttccacc gccagatagg tgtaatcatc tttcaggaac
     7021 tgcactaagt cagtgtcagg attgattaaa atcggcgtaa tgtgctgacg cagatactgc
     7081 ttaaaataat gacgcagcca gttttgttga ttgacggaga gctggcgttc attaatcagg
     7141 aagatctggt tgcgcgccat ctccagcaat agctcgttgt agaggccgtc gaattcctga
     7201 tcggctttca gcacccggga ctgaattttg cccagtaaat ggcgggaatg agagtgggag
     7261 ccttgttctt cgctaataat gatgcgtcgc ttcagttcag cgaagcggac tttatagaac
     7321 tcatcaaggt tattggaata gatccccagg aaacgcatcc tttcaatcag cgggttagat
     7381 ttgtccgccg cttcctgaag cacgcgttca ttgaacgata accaactgag ctctttttcg
     7441 atgtatagct tttcctgacc cattaccact tttactccgt tttattcacg ggacactgct
     7501 tggatattat ggcgagcaat attggcagat gtccaactat gccagaaaag ggggaaaaaa
     7561 actgctggag cttgcgattt acaggtgaat tgaaaaacgc cagcggcaga gctggcgctt
     7621 taactacggg gcattactcg tcggcagcgt agccctgcgg cggcagacgt tgaccatcca
     7681 gccacgcggc gttttcgtgc attttcagac gaccatcggc aaaccagcta atcaccagtg
     7741 gataaatggc gtgttcctgg gtttgcacgc gggcggtgac gtcatcctcc gtatcaccag
     7801 caaataccgg gactttcgcc tgtaaaataa ccgggccacc gtccagttca tcggtgacga
     7861 aatgcaccga tgtaccgtgc tcttcatcgc cattttccag cgcctgacga tggggatgta
     7921 atccgggata tttcggcagc agagaagggt gaatgttcag caaacgcccg gcatagtggg
     7981 agacaaatgc cgggctgaga atgcgcataa aaccagccag cacgaccaca tcgggtgcgt
     8041 acatgtcgat ttcatgaatc aactcccggt cataggcttc acgactgtca aacgcgctgg
     8101 cgatgagcgt atgcgttgca atacccgcct ggcgggcgcg ttcaaggccg aacgcgtcgg
     8161 ccttattgct gaaaactgcc cgtacggtgc ctttaatttt gttggtttta caggcgtcaa
     8221 taattgcctg caaattactt ccgttgccgg aaataagcac cacaatattc attattcgat
     8281 aaccacgcgt tgttcggaat cagaggcttt gatgataccg attttccacg cgttttcacc
     8341 gttggcattg agcagggcga gggctttgtc cacttccgga gcaggcaggg caataatcat
     8401 cccgacgccg cagttgaagg tgcgatacat ttcatggcgc tcaacgttac cagccgtttg
     8461 cagccagttg aacacttccg gccactgcca ggaggattca tcaatcactg cctgggtatt
     8521 atctggcaat acgcgcggaa tgttttccca aaagccgccg ccggtcaggt gcgcaatggc
     8581 attaacatcg accttttcaa tcaactccag cactgacttc acgtaaatgc gggtcggtgc
     8641 cagcagatga tcggctaatg gcttaccatc aagttcggtg gtttgcggat cacaaccgct
     8701 gacttcaaga attttgcgca ccagcgaata gccgttcgag tgcggaccgc tggaaccgag
     8761 tgcaatcagc acatcgccgt cgctgacttt agagccgtcg atgatttctg atttttctac
     8821 cacgcccacg cagaaacccg cgacatcgta atcctcaccg tgatacatcc ccggcatttc
     8881 tgccgtttcg ccacccacca gtgaacagcc cgattgcaga caaccttccg caatgccgct
     8941 gatcaccgct gaagcggtat caacatccag ttttccggtt gcgtaatagt cgaggaaaaa
     9001 cagcggttct gcaccttgca ccaccaggtc attaacgcac atggcgacca gatcaatacc
     9061 aatggtgtcg tgacgtttta agtccattgc cagacgcagc ttggtaccta cgccgtcagt
     9121 gccggaaacc agcacgggtt cacgatattt ttgcggcaat gcacacagcg caccgaagcc
     9181 gcccagaccg cccatcactt ccggacgacg cgttttcttc actacgcctt tgattcttcc
     9241 aaccagagca ttacccgcgt caatatcaac accggcatct ttgtagctaa gagaggtttt
     9301 atcggtcact gcttgggtcc ccacgcgtta cttgcggtag aaaaataaaa ttcggcgcaa
     9361 ttctaacagg gaaagcaaac gtttgcgaga ctgctttaca caaccttttt gcacgtcttt
     9421 tccccaggcg cgcggcgaaa gaagacttgt gccagggcaa aggttagttt tcggatggaa
     9481 taatcttctt tcataaccat ttgaatacaa aataacttta tctcaaaccg ttatcatttt
     9541 gactaaagt
//
LOCUS       DAZFBR010000152         9544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFBR010000152 DAZFBR010000000
VERSION     DAZFBR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.152"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(7..213)
                     /locus_tag="ACHJZ2_004341"
     CDS             complement(7..213)
                     /locus_tag="ACHJZ2_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4570332.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGARS
                     VAFIESEVDEWIQLTIENSRKHVA"
     gene            complement(313..1164)
                     /locus_tag="ACHJZ2_004342"
     CDS             complement(313..1164)
                     /locus_tag="ACHJZ2_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HFO4570333.1"
                     /translation="MKNWSVEHTREVKKWLDIDTYRGFEELPLIHLYHELLARTLFFK
                     PYHEEFEAEAVRLYIDRIFTGKPFLITEKHLGYLTREDTLYQPPHFFLTTTERLAQLS
                     IVGLRNSLFFWDGSDEYSVNREFLDSPVSEVLPKLFRDTVMVEIDLANGTDEEIAESL
                     KAALPQWRKVRGIEPDVTEAIRFGYGTIKKIINYRLLPMIDILVWSKLYKVRISEDRL
                     SRLLYTDDDDEINTRLNHQIRDTDKPLALKAASIPFIRQFNLFINKNSHLKKIRVSDV
                     MKIADSD"
     gene            complement(1591..6176)
                     /gene="ypjA"
                     /locus_tag="ACHJZ2_004343"
                     /pseudo
     CDS             complement(1591..6176)
                     /gene="ypjA"
                     /locus_tag="ACHJZ2_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
     gene            complement(6241..6360)
                     /locus_tag="ACHJZ2_004344"
     CDS             complement(6241..6360)
                     /locus_tag="ACHJZ2_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO4570334.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(6584..6742)
                     /locus_tag="ACHJZ2_004345"
                     /pseudo
     CDS             complement(6584..6742)
                     /locus_tag="ACHJZ2_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(7112..8461)
                     /locus_tag="ACHJZ2_004346"
     CDS             complement(7112..8461)
                     /locus_tag="ACHJZ2_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFO4570335.1"
                     /translation="MLINKSNGFNASAVWGSGSYNENKSSKHMELLAHSIVKLICKEA
                     ASETYRGALEILQKIMSECIYQEGNAFVIMGAGEQLKRIKYDVDENNLKVFNVHFDNN
                     EVLVTDGEPDVVCLSKQVWENLLIKLKPEIKENVASEVHKSANKGEIEQLVEWSKRNE
                     QTLFDNIIKSDFHVGSLKPGSMNGVILEMPPNVCMEPRNSYENKIDEVSSLSESEEHP
                     IDIQKITDAFVKEFKGILFDKNGRSSELLFNFYECCYTFLPRAQPQDKIDSYNSALQA
                     FSIFCSSTLTHNNVGFDFKLFPEVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEG
                     GLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGMSLFDVDTPCIFNTPANHESYEKSLKP
                     VSENGLNGVLSDRNKKIKMITGVAPFDDILFMDDDFDDNSPEDAPIENSPVVNSPLV"
     gene            complement(9213..9288)
                     /locus_tag="ACHJZ2_004347"
     tRNA            complement(9213..9288)
                     /locus_tag="ACHJZ2_004347"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9253..9255),aa:Ile,seq:cat)
BASE COUNT         2488 a         2285 c         1922 g         2849 t
ORIGIN      
        1 tttatttcag gcaacatgct ttcttgaatt ttcgatagtt aattgaatcc attcgtctac
       61 ttcactttca atgaaagcaa cagagcgcgc accgatttta acgggggaag gaaaacgatt
      121 ttggttgatg aggtgataga tccaagcttt accgtatcca gttcgatgca gaacctctgg
      181 taaacgaata aggcgggtgt tgtgctgatt catagtcgtt tcctctgtgc cgttttgatg
      241 gaaatgacta tacaaatgaa tcagcaggaa aaagaccgaa gctgggaacg ccagagctaa
      301 tggaggttgg tcttagtcag aatcagcgat tttcatcacg tctgaaactc tgatcttttt
      361 caaatggctg ttcttgttga tgaacagatt aaactggcga ataaaaggta tactcgccgc
      421 cttcaaagca agaggtttgt ctgtatccct gatttggtgg ttcaggcgag tgttgatctc
      481 atcatcgtca tctgtataca acaggcgaga aaggcggtct tctgatatac gtactttata
      541 taattttgac cataccagaa tgtcgatcat gggaaggagt ctgtaattaa tgattttctt
      601 aatcgttcca taaccaaagc gaatagcttc cgttacatct ggttctatgc cacgcacttt
      661 acgccattga ggaagtgcag cctttaggga ttcagcgatc tcttcatctg tcccgtttgc
      721 taggtcgatt tcgaccatga cagtatccct gaacaatttt ggcaaaactt ccgatacagg
      781 gctatcgagg aattctctgt taacagaata ctcatcgctt ccatcccaaa aaaacagtga
      841 gttccgtaat ccaactatgc tgagttgagc cagtctttcg gttgtcgtca aaaagaagtg
      901 cgggggctgg taaagagtat cctctcttgt gagatagccg agatgctttt ctgtgatgag
      961 aaagggcttt ccagtgaaga tcctgtcgat gtagagcctt acggcttctg cttcaaattc
     1021 ttcatgatat ggtttgaaga acaatgtcct tgccagcaat tcatggtaga ggtgtataag
     1081 cggaagctct tcgaatccgc gatatgtatc tatatccagc cacttcttca cctctctcgt
     1141 atgttcaact gaccagtttt tcataacgat ctccaacagg cattagactt aatgtatgta
     1201 gcaaacgtaa tcgacatgtt aaaggaagac caatgaaatg agttcagatg ccatcaggaa
     1261 gttgaaccaa aaggtttgat attttttgat acagttcgct aacggactat tttcaggggt
     1321 ttatgccgtt acgatttatg gttcctttat agaaccaaag gtaaagtcag aaacagcagt
     1381 tatctagaaa attcaatcaa atagatatct agttacaggg acgccagcca atcatgattg
     1441 gacggtgtaa ggacaacacc aacaaaaaca ggaagttaga agtctcagca ggacaccgac
     1501 cagacggtga ggagacataa aaggatacgc aaaggagccg cggctcctgg tgacatgaaa
     1561 gcccacagat gtgggctttt tcgttgatgg tcagaacgac cagttcacac cagctaccgc
     1621 gttccacggg gattccacac cggcaccatg gctatacccc accccaagat gcccgcttaa
     1681 cgtactgctg aatgaggctt taatacctgc ctggtatatt ccacgtctgc ccgacaaatc
     1741 attgacgaaa ttaccgtcat tattcacctt cacccggtta tcatcgacaa attctttgcg
     1801 cacagccgcc ttcagccacg gctcaatttc cataccgttc cccagacgca tgttgtaact
     1861 cagcgttgcg cccagttcac gatatatact gcgggtatcg actgatttcg attccatgcc
     1921 attggataaa tgatattcgg ggttatcagc ggtgaacccc gttaacgatg catacggcgt
     1981 caggttccag ttaccatcgg taaatcgcat cccggtttca atgtgaccgc ccagcccgtt
     2041 gctgtggtaa ctgccattgg cggctccacc gctgctcatt ttaccggcta cgttactttc
     2101 aaaacggttc agcttcacga caccgtccag atagaaacca ctttcatgtt cccaactggc
     2161 atagccgccc agagaataac tgcccacact gccatgtcct ccgcgatcaa aaccgatatg
     2221 tgaatgggaa taacccataa aagcgcccag cgtcgcaatc ccctcaggaa tatcattagg
     2281 gctgtcgatc cccactgtca ttccggtcag cgtctgctca aacccggccc ccgcatcggt
     2341 ggtgacatta ttacgggtgt tatacgtcgc cccccagaca ttattgttgt gtggactcgc
     2401 tttcattatg ttcaaccgct cgcgaatact gtttagctca gcatcaaata ccaacggtaa
     2461 tgttgctgcc atattgagta cggctgcggt agaaggcgtg atgcgtttct ccggaacggg
     2521 tgtcggcgtt ggctcgggag tcgggtctgg tttcggatcc ggttttgggt ctggttttgg
     2581 atccggcttc ggatttgggt tgggatttgg gttgggatcc gggttgggtt tgacatcatt
     2641 ggtcaggttc cagttgctgt tgccatcgct tttcaggaca tactcatagg tcccaagatc
     2701 aacgaaaccg ccagtattgc ccagcgaaaa cgaagcatcc cctccccctg ttttcaccag
     2761 cgtcatcgcg tcgtcagact gaggactgac gccggtatcc tgaacaaaga ttttaaaatt
     2821 accagtggcg ttgttgttga cgaccagttg atcgccccga gagcctgcaa cgttggtatg
     2881 caggtagaaa ttaccgctac cggaaagttc attggttgtc agcgtattgt atataccgtt
     2941 agttgtggca ggcgctgctg tttgtgctga taaataaaca ttaccgtcgt taagtagcaa
     3001 actgtttact ctatactcgc agttgctggt gcctgcacag gaattattgc tgttaagcca
     3061 gacactgccc cggctggcag ccgtcaggtc ggcaagcgtc gtatcaacgc cattgccgat
     3121 agttaatgtc gcgctatcgg taatccggat cgccccttcg agtttaactg gcgtaacatt
     3181 atcccgtggc gtcattaacg acaggcttcc tgtcgcgcca ctgaccgatg catccgccag
     3241 cgtacctgcg tagacgattg ctgtcccgcc agcaacctgg agatcttctg cccgggccag
     3301 agcctgaaac tcatcttttg accgcccaag ggtatattgc ccaccagagt taacctttgt
     3361 ggcggagtcc tgaccctggt tttgcattgc ccccccttgc caaccgtgga gtcgcgagct
     3421 tcggtacctg ctaataccaa taaattaccg ccattttcca gcaacatatt ggtcgctaaa
     3481 ttgccggaaa tggaaaaagt gccgtactgg tgagtaccgc tgatttcaat accgttagcc
     3541 gtgctcgtct ggagagcggc accgctgttc tggacgatat ctgtcgcttt gccattatcg
     3601 ttgactgtca gcgtaccgcc ttcattgatc gttgttttta ttgcctctcc gttagctgaa
     3661 actgtttgta ttccgccgtc gttaattgtt gtctcattcg ccacaccctc gacaatttgt
     3721 tcaccgccgg tgagcgtcgt gcctgtcgca gtggcttttg ttttgacgat ctcccgtccg
     3781 cccgtattga cctgtgtttt gtcagaagag gtgtctgact ccacggttaa cactccgcca
     3841 tttgccagca ggatattgtt cgccgcaccc tgctcgatgc tgaacgcgac gccatccgcg
     3901 cgtgttcctg tgacccgcgt cgccctggtg gttgcaacca acgcgccctg gctactctgc
     3961 tgtatccccg ttgcgctgcc tttctcccgc acatcgagtg tgccgccatc attaagcacc
     4021 gagttttcag ccagaccgcc ctcattaact atctgtgaac cgccattaat aatggaacct
     4081 tccgctgtcc cgtttgccat aatttgttgt aggccagaga cgatatcggt attgatcgcc
     4141 ttaccataat tctgaacggt ttgcgtgcca ccattgatgt gtgttttctc tgttgaccca
     4201 ccatcaacaa tttgttcacc actttcgata tttgcttcag tggctaaacc atataccgtt
     4261 tgcttgccac ctttgatatt tgctttatca gaagtggcac tggcatatat tgtttgggtg
     4321 ccagcactat tgagtacagt gccaacatct tttccataaa catccatttt gccgttggca
     4381 ttaataatcg tgtcaactgc ccgggaacca gtgacgactg ttaatgagcc agcgttttcc
     4441 agcactacat ttttagcttc tgaattcctg atgtagaaag catcaccata actgttggtt
     4501 ccttcgataa gtgttccgga ggtcgtggaa gcaattaatg cgccgccgga ttgttgctca
     4561 acatgcttag cctcaccacc gtcctgaacc tccagaacgc cgccattatt aagtctggtg
     4621 ctatctgttt tagcctcctt ctggacaatc agcttaccgc cagcatcaat ggtagtattt
     4681 ttcgccgagg ttttagccac taccgtcagt tcgccggtat tttccagcac aacataatta
     4741 gcctcccctc cggtaatagt gaagtgagag agcttgttgt atccttcgat atcagtccct
     4801 gcacccgtgt tggcaactaa agcactgccc gtctcctggt taaccccatg tgcaataccg
     4861 ccggtataga caatcagcga gcctccggcg ctaatattgc tgccaattgc cgtaccatct
     4921 ttctcaacaa cctgccggct cccggaggat ataattgttg tgtcagcttt cccgccgctt
     4981 ttgatatttt gcgttccgct gttgatattg gtgcctgtgg ctatgccata attattaata
     5041 ttctgtgtgc caccattaat tatggtattt gtcgcgtttc ctgcaacatc cataaccccg
     5101 ccattatcta ttcgggtcgc atcagcttta gcattggtta aaactgacat tgttccttta
     5161 tctttaataa tcgtcttgtt tgccgaacca tatgcgttta tgtctaaatg accaccgttt
     5221 tccagcaaca cattgtctgc cacgtgattg tggatggaga atgcaccttc actattcgta
     5281 ccgctcaccg tcgtaccgtt agtgttagtt tttaaaattg caccatcgtg ctgggtaaca
     5341 tttgttgccg taccaccact aacatcaagc acgccaccgg aataaacttc aataacatcc
     5401 gaggagctgg tgttatcaat aatttgcgta ccaccagaat agatctgagt attttttgcc
     5461 gttgacttac tattcagaga ctgagttccg ccttcaatcg tcgtgtccag cgcacggctc
     5521 tcatatactc tttgctcacc gccatttttg atggtggttg tttctactgt gctctgttca
     5581 acatactgtc gaccaccatt tatggttgtg ttcgttgcca gacttccttg tactacgtcc
     5641 tgagagccag acttatttat cgttgtacca tcagcatgcc cttgaacttt tactatctgg
     5701 ctaccaccat caatgagtgt tccattcgca ctccctccct ctacgcgtga agcaccgccc
     5761 ttaattgtcg ttccattgct gataccccct ttataaacgt cctgattgcc actctcgatt
     5821 atcgtacctg tggaaatacc cccgtcatga attgactgtc tgcccccgtt aatggtggta
     5881 ttattagact gccccacata attgttatgc cttcctacat cttgatatcc accagattca
     5941 ataagacttc cattagatac cccgccataa acattctgct ggccatggtt gataatatga
     6001 gtgttatttg ttgtacctcg ttcatctact cgttggctgc catctacagt ctcatcgttt
     6061 accacaccaa taacatcagg agtgaaggca gccatcccgg gcggggcata tatcaaggca
     6121 gatatcaata aggaaagtac tgagcgacga caataatagg gactggtcct gttcataaat
     6181 ttcatcctct gaaaagtgaa tactgagtag cgtttaagcg accttagctt tgctgcaaca
     6241 tcagcccaca ggcaccagac caggggattc atcctgaaga gacagcgcaa gtgtattgtg
     6301 ttcaccgctc atcaaagaca tcatgatgaa atgatgatat tccgcataag aatgaggcat
     6361 tttttaaacg cagtgcgctg aagtgtggtt ggataaaaaa gtcaatccat tcaggaaata
     6421 cgggcgtatt cttttctttc gacaatgagg ccgttggcaa aataaaatga tttacataat
     6481 cgtttctgat gaatatcttc tgctcacata aaaatcacac aataactttg agatcgcaga
     6541 ttgttttact tttacagcat tcgtcccccc attgttgggc aaatatagat tgggccagag
     6601 cacgaaagtt aataccacgt tcgcacagct cctccaacag cacgacaaga tgccacatac
     6661 tgcgccccag tcggttcagt ttacagacta gcagtgcttt ccctgccgat gatgtcctga
     6721 gcagtttttt cagtccggac ctcctgccac ggttttacgt tgaagcggta accctgagca
     6781 cgcagttctt cagtcaggcg tggtgcaccg taacgctgtt attgctgggt gagatcaaaa
     6841 aactttcagg cagctaagga aagttgaacc agacaataga tgaaatattt caaccaatta
     6901 cagcaccaat tcagtcactg ccagcccacc aaataaatca aggggttaca tgaaaacgta
     6961 gccccttttt ctttggtagt gacactaaaa tggatgtagt gtgaagaata atcccgttta
     7021 ctcaatcaat aatacatatt gtttcaatct acgttattat ctctttataa aaattgccat
     7081 ttatttatca ttgaaaactg cttttagaac tttatacaag aggactattc acaacaggac
     7141 tattctcaat gggagcatcc tcaggggagt tatcatcaaa gtcatcatcc ataaataaaa
     7201 tatcatcgaa tggtgccaca cccgtgatca tttttatttt tttattacga tcagataaga
     7261 ctccatttaa accgttttcg cttacaggtt ttaatgattt ttcataactc tcatggttag
     7321 caggcgtatt aaaaatacac ggagtatcaa catcaaacaa tgacattccc cagttcacat
     7381 attgaatatc atagttactg aagttctgtc cagaaaagaa gcatccctta aaatccaatc
     7441 cacgtagatt atataagcca ccctcttctt tttggagagt aatgttaatt ttggctatct
     7501 cccggacatc atcgccattt ttgtatttga ataccgtttc aagatgttct ccagacagct
     7561 tgacttctgg aaataatttg aaatcaaagc ctacattatt atgtgtcaac gtagatgaac
     7621 aaaagatgga aaaagcttgc agtgctgaat tatagctatc gattttatct tgaggctgcg
     7681 ctcttggtaa aaacgtatag caacattcat aaaaattaaa cagaagctct gaagatcttc
     7741 catttttatc aaataatatc cccttgaact ccttcacaaa cgcatctgtt attttttgaa
     7801 tatctatggg gtgttcctct gactctgaca aagatgaaac ctcatctatt ttgttttcat
     7861 atgaattacg tggttccata cagacatttg gtggcatttc taaaataaca ccattcatac
     7921 tacctggctt aagtgaacca acatgaaaat cactttttat tatattatcg aaaagggttt
     7981 gttcatttct tttagaccat tcaactaatt gctcaatctc acctttattc gctgatttat
     8041 gaacttcaga agccacattt tccttgatct ccggttttaa tttaatgaga agattttccc
     8101 agacctgctt gcttaaacat actacgtcag gctcaccatc agtaactaac acttcattat
     8161 tatcaaagtg tacgttgaat acctttaagt tattttcatc aacatcatat ttaatacgtt
     8221 ttaattgttc cccagctccc ataatgacaa aggcgttgcc ttcttgatat atacattcag
     8281 acattatttt ttgtaaaatt tcaagagcac cgcgatacgt ctctgatgca gcttccttac
     8341 aaattaattt tacaatacta tgagctagta gctccatgtg tttagaagat ttattttcat
     8401 tataacttcc actaccccaa actgcgctag cgttaaatcc gttgctttta ttaattaaca
     8461 ttttctattc ctcagttaat gtctacatgg ctatttttaa tgttattatt gtttgtcact
     8521 ataaaaaatc gctcatttga gacaattgct gacattaact gcttcacttg ctacgcatgg
     8581 aacttttaat taaattagca caggaatgtt aaatttaata aacaaaaggt tatttcgctg
     8641 tatgataaaa accacccgtt ataatttatt agtgaaaatc gtttttcgag tgttagaaat
     8701 ttatatctaa atagcgttga ttaatgagca tagccacgct cctgtaacgc tcacaaaact
     8761 catctacctg cggcgggtgt tctagtcagt agtagatgtt taaggcgtgg cagagacatt
     8821 tcatccttac tctacggcat tgttctacat acattggttg tggtactccc ttatcatcag
     8881 tgagcgaaca gagaatagtt cagtgatttg agtaattaac ctgattaaat gaatgggtat
     8941 aataaatgat aatacgctgg ctttatcgtt aattacttaa ttccacatgt aagcaatttg
     9001 cccgcttggc atagcaggca ttttttccaa atacttttga atgagtactg atagataaat
     9061 acattgcagt ggcgtgccac gtaccaaaac accagccctc attcgaaacc acccaccgca
     9121 cttcttcctt gaaatggcgt taatcgtgaa atatagaccg ccatcgagta acccttgtac
     9181 ccttaactct tcctgataca tgaatgatga tttggtggcc cttgctggac ttgaaccagc
     9241 gaccggacga ttatgagtcg cctgctctaa ccactgagct aaagggcctt aagtatgcaa
     9301 taacaatact tataacccac gcaataaaca tgatgatctt atgatgtaaa tcacagattt
     9361 ttatgcgttc tcatccatcc tgggtcgtca atacatgcga cataaaaccc gacaccacct
     9421 ccattccgca aagtcgatac tcgcagtcaa caagcaaatg ttaataatta gcacaatcta
     9481 tagttatcat cgattcaatg atactttgta atgattttgt atctaataat ataagtttat
     9541 taca
//
LOCUS       DAZFBR010000153         9421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFBR010000153 DAZFBR010000000
VERSION     DAZFBR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.153"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(450..980)
                     /gene="yodB"
                     /locus_tag="ACHJZ2_004348"
     CDS             complement(450..980)
                     /gene="yodB"
                     /locus_tag="ACHJZ2_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO4570336.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIVAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1323..1973)
                     /gene="zinT"
                     /locus_tag="ACHJZ2_004349"
     CDS             complement(1323..1973)
                     /gene="zinT"
                     /locus_tag="ACHJZ2_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO4570337.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2230..2865)
                     /gene="msrQ"
                     /locus_tag="ACHJZ2_004350"
     CDS             complement(2230..2865)
                     /gene="msrQ"
                     /locus_tag="ACHJZ2_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO4570338.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            complement(2866..3870)
                     /gene="msrP"
                     /locus_tag="ACHJZ2_004351"
     CDS             complement(2866..3870)
                     /gene="msrP"
                     /locus_tag="ACHJZ2_004351"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO4570339.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(3978..4391)
                     /gene="hiuH"
                     /locus_tag="ACHJZ2_004352"
     CDS             complement(3978..4391)
                     /gene="hiuH"
                     /locus_tag="ACHJZ2_004352"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO4570340.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4524..5195
                     /gene="hprR"
                     /locus_tag="ACHJZ2_004353"
     CDS             4524..5195
                     /gene="hprR"
                     /locus_tag="ACHJZ2_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO4570341.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5195..6553
                     /gene="hprS"
                     /locus_tag="ACHJZ2_004354"
     CDS             5195..6553
                     /gene="hprS"
                     /locus_tag="ACHJZ2_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO4570342.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(6661..7512)
                     /gene="hchA"
                     /locus_tag="ACHJZ2_004355"
     CDS             complement(6661..7512)
                     /gene="hchA"
                     /locus_tag="ACHJZ2_004355"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO4570343.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(8104..9290)
                     /gene="ompC"
                     /locus_tag="ACHJZ2_004356"
                     /pseudo
     CDS             complement(8104..9290)
                     /gene="ompC"
                     /locus_tag="ACHJZ2_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
BASE COUNT         2613 a         1953 c         2172 g         2683 t
ORIGIN      
        1 tgattcgcta tggatctgac aggaagcttt cgagcgaaaa tgtgcagtta ttcagtcgtt
       61 ttcttatcgc tcaccattat tcttttagac attcatccta tgaaactgcc gcaaagttgg
      121 tggtgggaac tgaagttgcg tagagaaggg gtcaagaatt gagtcaaaaa aggaacgcgg
      181 ccgtcagatt aaagccgcgg gacaaagtcc ataatttcca agagttgttg aatttcccag
      241 cttttttgaa agcctttgta tcatactaac aagtaaaaaa ggtttaaata ccaaaattaa
      301 cctgtatttt tgataagtct taccattgta cgatttacac attttctgct cctccttgtt
      361 gatagtggaa agagtgcggt atgcacttgc gtaaacattt atgatgcgtt gatgtccatg
      421 gttcacattt cctttgtcag aggatcgact tatttgcggc gaggcatcat tcgtaacaga
      481 gtattatctt tctgaatgta gtgatgaaag agtgctgcgc cagcgtgagc tacgattaaa
      541 aagtagccta tatttgccca ggtttcgtga atatttttta tcagtgcttt aatttcgctg
      601 tttggggtaa caaagggaga cacattgaaa ccaaggaaac tccacgattt tccactgtaa
      661 gccatcaaag caatccccag cagaggaagc gcaaggaagg ttatataaag catgatgtgc
      721 attagcgaag ccgctttcat ttgccaggca ggtggcggtg gcacaataga aggatcacta
      781 taacggtgtt ttataatcag gcgtgaaaac attaacaccc aaacgaatat acccgcattg
      841 taatgtgttt ctcgcatcag cagataagta ctactacctt taggaaacca gccacggagt
      901 tccatcgcgg cataggttat tgcaacgaaa agcagcgtta tccaatgtaa ataaatttga
      961 gtttttgaga atcggttcat ttttgtagct cctgtaattt tcacagtcaa gctatcccac
     1021 gaaccttaaa aattccttat acagatgtgg ttttaagaat ttgttattta aaaaattgat
     1081 agtgcacatt gtaaccagcc aatataagca atggcgtgag cacaggtatc tttctctgtt
     1141 ggccgtattg tgtatggaat ccgttattgg ttaactcaat gcgggttagc ttattaatat
     1201 acagacacgt catagtgatg agcgtgtctg tagctagctt gcgttcagtg gcgtttgtgg
     1261 atggaccagt ggcagttatg atgacatact aaagcggcat cgaggcgtta tcatgagaat
     1321 actcaatgag acatcatttc ctcgaccact tcttcgctac tcaactgata tggataatac
     1381 gttggccagt tttccatttc atttaataaa gattgctgtg aatcattacc cataaaaatg
     1441 tgaaaatgag atgattttcg tggtgcaatt atatgatcgc taaattgtat atatttaggg
     1501 gctttgcttt caggatcttt acattcgaat aagtaacgaa cgcctttctt gcctgattta
     1561 taggtgagta ttttgtatcc atcgtaatca tatttacagg atgttgtttc attatttcta
     1621 tggaattcaa caatgccgtc ctcaatgcca atcatctcga tatctgttgc atagcctttg
     1681 tgataataat ctttaatttc agcaaatgtt ttagttttat ctgcatccgc tttcttctga
     1741 aagacggggg caagtttgcc actttgcagt aaaggataaa cggattgcca gactccatcc
     1801 cagtcactga gcgttcggtt ttgtacattg gcatcatcaa aaacaccatt agcagctttt
     1861 tgttcgacct ctgttaaggg tttgccgtgt gagtgatgac catgcgaaaa ggcaggagcg
     1921 ctaacaataa agacacctaa agcaacagcc agtttgtgaa gacgaatcgc caaaacagtt
     1981 tcctccagag tcattgtata tgatgtgtaa tgttatattg taacatatga caaaatgcaa
     2041 caactcttag gagatgagag catagttttc tcaggtgtac ttatctatct tattgaaata
     2101 aaatgaatta tgttgataat ttgaggttga tgacaaacga tacataacct gatgtgatct
     2161 gcgtaggccg gatagggcgc tcgcgccaca tccggcaggg ataacgcgta ctttgtcggc
     2221 aatatgaagt tatacagata atttgttgtg aacctgcttg cgtaaccggt taaaaaggga
     2281 acgcgacttc ttataccgta aggctaaaag cagtacagcc agcccagcgt agatgagcgg
     2341 ctgcggtgaa ataatcttca ctgaccacag ataatgtatt ggggccagga tcgcgacaag
     2401 atagacgaaa ttatgcaact gttgccagtg cttgccgagt tttcgctgca tcgcctgggt
     2461 tgaagtaaac gctaaagcaa gcaaaataat ccagctgata attcctaacg ttaaataagg
     2521 ccgggtaatt aactccttgc ctaataacgc cagattattc acgcccagct ccagcaatgc
     2581 gtaactggtt aaatgcagtg tcgcccaggc aaagcaccat aagcctaaca ggcggcgtgt
     2641 gcgtatcaat aacggctgtt ttgcgtagcg tgccagaggt gtgattaaca aggtcgccag
     2701 caaaaatttc agtgcagtgc gaccggtaaa atgctgaata tctttcaccg gatcggcacc
     2761 cagtccaccg tgattgatcg cccagaccag ccagagaaac ggcaacaatc cggcaagatg
     2821 caggcaaact ttcagccatg tcacctgttt tgctgtcaga cgcattcaga aattctcccg
     2881 caaatccagg ccacgataca gcgatgccac ctggtcggcg taaccattaa acagtagcgt
     2941 tggctggcgc tgtacatcga ggatgccgcc tgaaccaata aatcgttcgg tagcctgtga
     3001 ccagcgtggg tgatcaacat gcggattaac gttggcgtaa aaaccgtatt cgtcaggcgc
     3061 tgccagattc caggtggttg gcggacgctc gcgggtcagc ttaatactga cgatcgattt
     3121 aatcccttta aagccatatt tccacggcac aatcagtcgc accggcgcgc cattttgtgg
     3181 cggtaacgcc ttgccataaa cacctacggt catcagtgtg agcggatgca ttgcttcgtc
     3241 gagacgcaat ccttcgacat aaggatattt cagcccgccg ccgataaagc ggtcctgctg
     3301 gcctggcatc tgttccggtg cataaattgt ttcgaaagcg acatacttcg cattgctggt
     3361 gggttcggca agcgccagca atttgtgcag cggaaaacca atccacggca ccaccatcga
     3421 ccacgcttcc acgcagcgca tacgataaat acgctcttcc agcgggaagc gacgggttaa
     3481 atcatcgtga tcgagggtca atggttttgc cacttcgccg ctgattttca gtgtccatgg
     3541 atcggttttc aggctaccag cattagcggc gggatcggct ttatccagcc cgaattcata
     3601 gaagttgtta taaccggaga ctttatctgc tggcgtcagt ggcaggttat tttgccaggc
     3661 ggcaggcttg ctgaactcca gcgcttttcc ggcgggggcg ggtgggcgat cgttcccttt
     3721 aaaccagcta agcagatcgg catgcgcagc gtgaggcaaa gaaagtgcag ctgcgctgat
     3781 gcccagtgct tttaacacct gccgacgctt cataaagaat accgactcgg ccgtaacatc
     3841 tgattctttt aaaaattgat tctttttcat aagccatcac actttttgtc gttagccagc
     3901 aagagccgtt tactgttaat ctgtcaggga gtgtcctccc taacagtagc agagatctgc
     3961 ttttacatat ttatgcatta actgccacga taggttgaat acccatattg gctaagtaat
     4021 aaaggcacat gataatgctc gttcacttta ttaatatgaa attcaacggg gatctcaggg
     4081 aagaaacttt caagattttg tttcttgaaa tagtccccgg ttttaaatac gacacggtaa
     4141 tcgcccgtag ttgcagtttg ctcgggccac agtgccttaa ttcgtccatc cttatctgtt
     4201 ttggcggtat taagttgtaa ccagccgttg tccgctttct tttcaagagt gactgtcacg
     4261 tcggcagcgg gttttcctgt ttgctggttc aaaatgtgca cgctaagaat gttttgttgt
     4321 gcagcataga ccaaagaagg taatgaaaat gcagccgtta ctacggagag tactaaataa
     4381 cgctttaaca tgtttatatc cttgtcatgt gaatgagttt tgaggatagc gaaaaagcgt
     4441 ccagggtaac cttacagcag cattacaatt ttgtaatgaa ccaggctgtt tctatgacat
     4501 atgatttatg gcatattatt ttcatgaaga ttttacttat tgaagataat caaaggaccc
     4561 aggagtgggt aacgcagggg ctttccgaag cgggttatgt cattgatgcc gtttctgatg
     4621 gcagagatgg gctttatctt gcgctgaagg atgattatgc attgatcatt ctggatatta
     4681 tgcttccggg tatggatggc tggcagatct tacaaacgtt aagaacggca aagcaaaccc
     4741 ctgttatttg ccttactgca agggattctg tcgatgacag agtcagaggg ctggacagtg
     4801 gagcaaatga ttatctggta aaaccttttt cattttctga gttgctggca agggttcggg
     4861 cacaattaag gcaacatcac gctttgaatt caacattaga aatcagcggc ttaagaatgg
     4921 actctgttag tcaaagtgtg agcagggaca atatcagtat tacactgacg cgcaaggagt
     4981 ttcagttact ttggctactg gcctccagag ctggcgaaat tatacccaga acggttattg
     5041 cgagtgaaat ttggggaatc aactttgata gtgataccaa tacggtggat gtcgccattc
     5101 gcaggctccg cgcaaaagtt gatgatcctt ttcctgaaaa gctaattgcc acaatccgtg
     5161 ggatgggata ttcgttcgta gcggtaaaaa aataatgaaa agattatcta taaccgtccg
     5221 tttaaccttg ctttttatat tgctgctgtc tgttgctggc gccggaattg tctggactct
     5281 ctataatggc ctcgcaagtg agttgaaatg gcgcgatgat acaacactca ttaaccggac
     5341 agcgcagatc aagcagttat taattgatgg ggtaaatcca gatacgttac ctgtatactt
     5401 taaccggatg atggatgtta gtcaggatat cttgatcatt catggtgatg gcatcaataa
     5461 aattgttaac cggacaaatg tcagtgatga catgttaaat aacatacctg ctagtgagac
     5521 aatcagcgca gctggcattt acagaagcat tattaatgat acagagatag atgctttacg
     5581 aattaatatt gatgaagttt cgccatcatt aacggttact gtggctaaat tggcttcagc
     5641 cagacataac atgcttgaac agtataaaat caatagcatt ataatttgca ttgtcgccat
     5701 tatactgtgc tcagtattaa gtccgctgtt aatccgaacg gggttacgag agatcaaaaa
     5761 gttgagtggt gtaacggaag cgctgaatta taacgatagc cgggagcctg ttgaggttag
     5821 cgcattaccg agagaactaa aacctcttgg gcaggcgttg aataaaatgc atcatgcttt
     5881 agtcaaagat tttgagcgtc taagtcagtt tgctgacgat ctcgctcatg aacttagaac
     5941 gccaattaat gcattactgg gtcagaatca ggttacgctc agtcaaacca gaagtatcgc
     6001 tgaatatcaa aaaacaattg ccggtaacat tgaagagctg gaaaatattt cgcggttaac
     6061 agagaacata ctgtttcttg ccagggcaga taaaaacaat gttttggtga aactggactc
     6121 gctttctctc aataaggaag tcgaaaattt gttggactat cttgaatatc tttcagacga
     6181 gaaagagatt tgctttaagg tcgagtgcaa tcagcaaatc tttgcggata aaattttact
     6241 gcaacgaatg ttatcgaatc ttattgttaa cgctattcga tattcgccag aaaaatcgcg
     6301 tattcatata accagttttc ttgataccaa cagctatctt aatattgata tcgccagccc
     6361 tggagcgaaa attaatgagc ctgaaaaact cttccgtaga ttttggcggg gagataattc
     6421 gcgtcattcc gtaggtcagg gacttggtct ttctttagtc aaagcgattg ccgaattgca
     6481 tgggggaagt gctacgtatc actatctcaa taagcataat gtgttccgga ttacgttgcc
     6541 gcaaagaaat taacataggg cttcagtacg ccataaatta accgtgaaaa aacgcagcca
     6601 cgtcgtgagt actaacgcgg ctgcgattga ttatgcgctt acattcaaac gtaacaggga
     6661 ttaacccgcg taagctgcca gcatttcctg cgccgccagt ttacccaacg cattcgctgc
     6721 aaaaggactg tcgccggtga gaagtttacg gtccttatgt actcgcccgg tgatgtcgtc
     6781 attaatgatg ttcatgccca ttttcttcag ttcttcgccg aagtaccagg tgagatgacc
     6841 cggcatatag ccaatctctg gcgtttgttt gtctgcggcg tctgggaatg cgcaaatgga
     6901 ataaccattc agtggattat cgccgtggcg aagcgccaga aaagccgccg ggccgtggca
     6961 aagggagata acaaaacggt catttttgat tgcccactgt aaagcggcag ctacgtcctg
     7021 gctttcaggt aaaccaataa gtgcgccatg accaccagga acaaagattg ctgcatattc
     7081 gctatcagcg ttgaggctgg cgacaacatc cgcgagtttc ttcggattgc ggaacaacga
     7141 tttatgctgc tcaaagaatg gcattacttt ttcatctttc tgcggcatag cccagtattc
     7201 aaacttggtc atcagaccgg aaatggtcgc cacttcgaat tcgaaacctg cagcatggag
     7261 atgatacaac ggcagcaacg tttcaatcgg atggttaccg gtcgagaaca gttttccgtt
     7321 atcggtcggc aaataacgtt cgtccgcggc gatcaccaga attttatgtt taccgcgata
     7381 aggttttgga tagtccacgc catcaagatc agagacagga ctggtatatt ggctaagcga
     7441 atattctgaa gggaagaatg cattatcttc agcaatatcg acctgcggat ttttactggt
     7501 ttgaacagtc atagtgtatt ccttattgtt gcttagttag ggtagtcact atatttgcga
     7561 ctgagctggt cgctattttt aaccaggctg gaaaattgag tcaatatatc gaaagcggta
     7621 gtcggcgggt gtaagtatgt aatgatgatg ctgcactggt gttgaatatt attcacgcca
     7681 aattttcctt attgagcaac ttcgtcgcaa gtggcctcaa tgattttaga ccattatcag
     7741 aggtacgggg cggcggggag agatttagtg ctgtggaata ttgtggtgct gatgaatatt
     7801 tatatcgcgg attggtagag aaaaagctta tttcatgcac tatttttgca aatatttcta
     7861 tatgggaagc gtgtttgtta cggattaatt cataaagaaa tgcatttggg tgttggcttg
     7921 aagagtcgct ggttgatgtt attgtgccag gaggggcagg gtaagggggt gtatcgataa
     7981 gctttggcaa ttgcgtgtgc cgcagatgga gatgaaatgc caataaaaaa gcctgagttc
     8041 gatgagggac gaactcaggc tagtgttcag ttttacaata acacgccgcg gcgcgttggc
     8101 tgattagaac tggtagacta agcccacagc cacgatatca tcggtagcga tgccgttttc
     8161 tttgtagaag tcgtcgtcgt tgtccagcag gttgattttg taatcaacat aagtggacat
     8221 gtttttgttg aagtagtagg tcataccaat atcaacatat ttaaccagat ctttgttggt
     8281 gtaatgatga ttaccctggc tgtcgaagtt atcactattt acaccgccta aatccttacc
     8341 tttagattgc agataagcga tggacgggcg cagaccgaag tcgaactgat actgagcaac
     8401 aacttcaaag ttttgggttt tgttagcgat ggcatcagca tcaccgtaac cagtcatgtt
     8461 acggggttca gcatacatag ctgctaagta aacgttatta gcgtcgtatt tagcaccaac
     8521 ggtccatgct tctgctgttt caccgccagc agttgcatcg aagcgatctc cataacgagt
     8581 gttatgagta cctttgtgaa cctgattgtc ggtacggtca gagttagagt aagcagcgcc
     8641 cagacttaaa ccaaagtcaa agtcatagga agtggacata ctgaaccgtc gccgttctct
     8701 ttagcaagtt tacgtccatt accgttacct gaaccttctt gatcaaataa tccgcttttc
     8761 tcgttgttac cctgatactg gagtgcaaag ttcagaccat caacttgacc aaagaaacca
     8821 ttgttacgat aggttgctac gctgttagca cgaccgttca tgaagttgtc agcgccagca
     8881 taggtatctc caccaaattc tggcagcata tcggtccatg cttctacgtc atatacgaca
     8941 ccgtagttac gaccatagtc gaaagtacca ttctgaccaa agccgaaacc tgcaaatgcc
     9001 agacgcgttg cggtattacc cttgtcgctt tcaggaccgt ttgcgccaat gttaaattcc
     9061 cactgaccat aaccagtgaa ttggtcagcg atctgagttt cacctttgaa accgatacgg
     9121 gcatatgaca tgtcgccatc gctgctagca tcatcagaga agtagtgcag gcccgctact
     9181 tttccgtaca aatccagttt attgccatct ttattataaa tttcagccgc atttgctgcg
     9241 ccagcaacta ataacgccgg gaccagcatt gccagaactt ttcttttcat tatgtattcc
     9301 cttgtgatta taatcttcat gaatatatca ataagtgccg ttatccaaaa aaaagcacat
     9361 ttggatacta ttctatgaag ttcattttat tttaaagatt acatgcaaca aatatattta
     9421 a
//
LOCUS       DAZFBR010000154         9405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFBR010000154 DAZFBR010000000
VERSION     DAZFBR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.154"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            71..1459
                     /locus_tag="ACHJZ2_004357"
                     /pseudo
     CDS             71..1459
                     /locus_tag="ACHJZ2_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            1479..2225
                     /gene="traX"
                     /locus_tag="ACHJZ2_004358"
     CDS             1479..2225
                     /gene="traX"
                     /locus_tag="ACHJZ2_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO4570344.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            2284..3144
                     /locus_tag="ACHJZ2_004359"
     CDS             2284..3144
                     /locus_tag="ACHJZ2_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4570345.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            3247..3807
                     /locus_tag="ACHJZ2_004360"
     CDS             3247..3807
                     /locus_tag="ACHJZ2_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO4570346.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            3940..4152
                     /locus_tag="ACHJZ2_004361"
     CDS             3940..4152
                     /locus_tag="ACHJZ2_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO4570347.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            4397..4858
                     /locus_tag="ACHJZ2_004362"
     CDS             4397..4858
                     /locus_tag="ACHJZ2_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO4570348.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            4904..5113
                     /gene="hha"
                     /locus_tag="ACHJZ2_004363"
     CDS             4904..5113
                     /gene="hha"
                     /locus_tag="ACHJZ2_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO4570349.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            5151..5489
                     /locus_tag="ACHJZ2_004364"
     CDS             5151..5489
                     /locus_tag="ACHJZ2_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570350.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
     gene            5729..5983
                     /locus_tag="ACHJZ2_004365"
     CDS             5729..5983
                     /locus_tag="ACHJZ2_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO4570351.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCV"
     gene            6219..6293
                     /gene="tap"
                     /locus_tag="ACHJZ2_004366"
     CDS             6219..6293
                     /gene="tap"
                     /locus_tag="ACHJZ2_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO4570352.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            6286..7143
                     /gene="repA"
                     /locus_tag="ACHJZ2_004367"
     CDS             6286..7143
                     /gene="repA"
                     /locus_tag="ACHJZ2_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO4570353.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            8055..8339
                     /locus_tag="ACHJZ2_004368"
     CDS             8055..8339
                     /locus_tag="ACHJZ2_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO4570354.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            8339..8614
                     /locus_tag="ACHJZ2_004369"
     CDS             8339..8614
                     /locus_tag="ACHJZ2_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4570355.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            8709..8915
                     /locus_tag="ACHJZ2_004370"
     CDS             8709..8915
                     /locus_tag="ACHJZ2_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570356.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLDR"
     gene            complement(9015..9230)
                     /locus_tag="ACHJZ2_004371"
                     /pseudo
     CDS             complement(9015..9230)
                     /locus_tag="ACHJZ2_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9274..>9405)
                     /locus_tag="ACHJZ2_004372"
     CDS             complement(9274..>9405)
                     /locus_tag="ACHJZ2_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4570357.1"
                     /translation="NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT         2431 a         2208 c         2611 g         2155 t
ORIGIN      
        1 gtatttttag tcatcctgtt tacctctttc tcagggagtt tagtctccag gatttccggg
       61 gcggttcaat caccggattg ccggtatcag cagggatgac gggctgataa ctctgcagga
      121 tgcggaaggt aacacgcggc tgatttcgcc ccgggaggcg gtggctgaag gcgtcacact
      181 gtaccccccg gacaccatca gggtggggac cggtgaccgg atgcgcttca cgaagagtga
      241 ccgggggcgt gcttatgtgg ccaacagcgt ctggacggtg acagcggttt ccggtgacag
      301 tgtcacgctg tcgggcggac agcagacccg ggtgattcgc cccgcccagg agcgggcaga
      361 gcaacatatt gacctggcct atgccatcac cgctcacggt gcgcaggggg caagtgaaac
      421 ctttgccatc gcgcttgaag gtacggaagg cagccggaaa ctgatggccg gctttgagtc
      481 agcctacgtg gccctgtcgc gtatgaagca gcatgtgcag gtgtacaccg ataaccgtca
      541 gggctggacg gatgccatta acaatgccgt acagaaagga acagcccacg atgtgtttga
      601 accgaaaccg gaccgggagg tcatgaatgc agagcggctg ttcagtacgg cgcgggagct
      661 gcgggacgtg gcggcagggc gtgccgttct tcgtcaggcg gggcttgccg ggggagacag
      721 tcctgcacgg tttattgctc cgggacgtaa atatccacag ccgtatgtgg cactgccggc
      781 gtttgaccgt aacggcaagt ctgccggtat ctggctgaac ccgctgacca cggatgacgg
      841 aaacgggctg cggggattca gtggtgaagg acgggtgaaa ggcagcgggg atgcgcagtt
      901 cgtggccctg cagggcagcc gtaacggaga gagcctgctg gctgataata tgcaggatgg
      961 tgtccggatt gcccgtgata atcctgacag cggtgtggtg gtgagaatcg ccggtgaagg
     1021 tcgtccgtgg aatcccggtg ccatcaccgg tggtcgcgtg tggggggata tcccggacaa
     1081 cagcgtccag ccgggagccg gaaatggcga gccggtcacg gcagaggtac tggcacagcg
     1141 gcaggctgaa gaggccatcc gccgtgaaac ggaacgccgc gcagatgaaa ttgtccgtaa
     1201 aatggcagag aacaaacctg acctgccgga cggcagaaca gagcaggctg tcagggagat
     1261 tgccgggcag gagcgtgaac gggctgtcac ttctgaacgg gaagccgcgc tgccggagag
     1321 tgtactgcgt gaaccacaac gggagcggga ggcggtccgt gaggttgtcc gggaaaacct
     1381 gctgcaggag cgactgcagc agatggagcg ggatatggtt cgtgacctgc agaaagagaa
     1441 aaccctgggc ggagactgat acaggaagac aaacactgat gacaacggat aacacgaaca
     1501 cgacacgtaa cgattcactg gctgcccgga ccgatacctg gttgcagtcg ttgctggtct
     1561 ggtcacccgg acagcgggat atcatcaaaa cggtggcact ggtgctgatg gtactggacc
     1621 acgcaaaccg cattctgcat ctggaccaga cgtggatgtt tctggccggg cgtggagcct
     1681 ttccgttgtt tgctctggtg tgggggctga atctgtcccg ccatgcgcat atcaggcagt
     1741 cggccattaa ccggctgtgg ggatggggaa ttattgccca gttcgcgtat tatctggcag
     1801 gctttccgtg gtatgagggg aatatcctgt ttgcctttgc agtggcggca caggtgctga
     1861 cgtggtgtga aacgcgcagt gggtggcgta cagcagcggc cattctcctg atggcactgt
     1921 gggggccttt gtccggcacc agttacggca ttgccgggct gctgatgctg gcagtcagcc
     1981 accggctgta ccgggcggaa gacagaacgg aacgtctggt gctggttgca tgcctgctgg
     2041 ccgtgattcc ggcacttaac cttgcctcca gtgatgcggc ggctgtagct ggtcttgtga
     2101 tgacggtgct gaccgttggt ctggtgtcct gtgcagggaa atcattaccc cgtttctggc
     2161 ccggagattt tttcccgacg ttctatggct gtcatctggc tgtgctgggc attctggcgt
     2221 tgtgactggt atgatactga cgctgattct gcttattaat aaacaacata acacaggata
     2281 tttatgaaaa ttacggatca caaactctct gaaggtattg ccctgacatt tcgtgttcct
     2341 gaaggaaata taaaacatcc tttgattatc ttatgccatg ggttttgcgg tatcaggaat
     2401 gttctgctcc cttgctttgc gaatgccttc acagaagcag gattcgcaac gattactttt
     2461 gattatcgag gattcggtga gagtgatgga gaacgagggc gtctggttcc tgcaatgcag
     2521 accgaagata ttatttcagt tatcaattgg gcagaaaagc aggaatgtat tgataatcag
     2581 cgtattgggt tatggggaac atcgctggga ggggggcatg tcttcagtgc agctgcgcag
     2641 gaccaacgcg taaaatgtat tgtcagccag ctggcttttg ctgacggtga tgttctggtt
     2701 accggagaga tgaatgaatc ggagagagcg tcttttctat ccacgctgaa taaaatggca
     2761 gagaagaaga aaaacaccgg gaaagagatg tttgtggggg ttacacgggt actaagtgat
     2821 gatgaatcga aggtattctt cgagaaaatc aaagcccggc atcccgaaat ggatataaaa
     2881 atacctttcc ttacggtcat ggaaacgttg caatataaac cggcagagag tgcagccagc
     2941 gtccagtgcc cagttttggt tgttattgcc ggccaggata gcgttaatcc accggaacag
     3001 ggaagagcat tatatgatgc tgttgcttct ggtactaagg aattatacga ggaagctgat
     3061 gcatgccatt atgatattta tgagggcgcg ttttttgaac gtgtagtagc tgtgcagaca
     3121 caatggttta aacaatatct ttaattgtca gtatttatac gttaaaggtc gttgaaatta
     3181 acttcctgtt acgatgagcc gtttcaacgg cctgcagggc atattttcac gaaggtcaga
     3241 gtaagcatgg cagagcaaaa acgaccggta ctgacactga agcggaaaac ggaaggagag
     3301 acgcctgtcc gcagccggaa aaccatcatc aatgtcacca cgccaccaaa atggaaggtg
     3361 aaaaagcaga agctggccga gaaagcagcc cgggaagcgg agctggcggc aaaaaaagcg
     3421 caggccagac aggcgctgtc catttatctg aacctgccca cgcaggatga ggccgtgaac
     3481 accctgaaac cctggtggcc gggattattt gacggtgaca cgccccggct tctggcctgc
     3541 ggtatccggg acgtgttact ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa
     3601 aagctgcgca gggcgctgaa ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa
     3661 gccggtgcct gccggtatga cacggaaggg tatgtgacgg agcatatttc tcaggaggag
     3721 gaagcgtatg cggcagcgcg tctggataaa atccgccgcc agaaccggat aaaggcagaa
     3781 cttcaggccg tgcttgatga gaaataaaaa aagcctccct cagagagagg aggcagggaa
     3841 ataaggctta aaaggaaata acttccgtac ccgaaaggaa gccaattcag tagattattt
     3901 aacaccataa tactgacggg ttaaagagga gtgataaaga tgaatggatt cagaaacagt
     3961 tcacggaacg gtcaggtctg gcgttaccag cgtgccggaa gccgggccgt tattctggaa
     4021 gtcagtggac gctggatgga agcggcagaa gcatggcgac gggctgccgg tgttgcaccc
     4081 cggactgact ggcagcagtt tgcccgaaaa agggctgaac actgccaccg gcgctgccga
     4141 ggaagggggt aacgaaaaaa gcagccttca ttactccagg gattcagaag gctgaataat
     4201 gcatgagatt tgcttttttt aatatccggt cgggcagtat gaccggccag ttctgagtag
     4261 cacagagttt tttccgtatt aagtcgtgat tttatatttt gtgattaatt tcacaaaata
     4321 aggtgttatt aagtgtgtgc tgcaatattc aggatgcctg aaatacaggt gctatttatc
     4381 tgatacggag aataacatga ggaaatatat tccactggta ttatttatct tttcatggcc
     4441 ggtattgtgt gcagatattc atggacgggt tgttcgtgta ctggacggtg ataccatcga
     4501 ggtcatggac tcacggaagg cggtgcggat caggctggta aatattgatg cgccggaaaa
     4561 gaaacaggat tatggccggt ggtcgacaga catgatgaaa tcactggttg caggaaaaac
     4621 ggttaccgtc acatattttc agcgagatcg ttatggtcgt atgctcgggc aggtttatgc
     4681 accagacgga atgaatgtta atcagtttat ggtccgggcc ggggccgcct gggtatatga
     4741 gcagtacaac acagatcccg tgctgcctgt ccttcagaac gaagccagac aacaaaaacg
     4801 tgggctctgg tcagatgctg atccggttcc cccctggata tggagacacc gtaaataaag
     4861 cgatggcaat cttatcattc atctgttctc agcgggaata aaaatggaaa aaacaaaaca
     4921 ggaatggctg tatcaattac ggcgatgttc atcagtcaat acgctggaaa aaattattca
     4981 taaaaacaga gacagtttat cgaccagtga acgtgaatca tttaattcag cagcagacca
     5041 ccggttagca gaattaatca ccggaaagtt atatgacagg ataccaaaag aaatatggaa
     5101 atatgtaagg taatagttca gcataattaa ttaccaggag cagataaaca atgaagctga
     5161 ttatttttat tctcattgtt ctgattattg cagcgttact tatccggatt atcctcagaa
     5221 gtgtaaatca gcattctcct ttgctgatgc aattgcatgc ggcaggtata cggaccggtg
     5281 atgcagagcg aatactgtcc ggcggtgaat atgatgccag ccatttacga cctgaacgca
     5341 gacgccggga tattcttctg gaagaggttc tgaggcaggc tggtattccg ttgctcagaa
     5401 gccacgatgc cagaaaactg ctgcagatga ccggagaatg gctgaataca acaggggctg
     5461 ctcagcagtc accggaacat cgtagctgac gccttcgcgt tgctcagttg tccaaccccg
     5521 gaaacgggaa aaagcaagtt ttccccgctc ccggcgtttc cataactgaa aaccatacta
     5581 tttcacagtt taaatcacat taaacgacag taatccccgt tgatttgtgc gccaacacag
     5641 atcttcgtca caattctcaa gtcgctgatt tcaaaaaact gtagtatcct ctgcgaaacg
     5701 atccctgttt aagtattgag gaggcgagat gtcgcagaca gaaaatgcag tgacttcctc
     5761 atctggcgca aaacgagcat acagaaaggg gaatccgctt tctgatgcag agaaacaaag
     5821 attatcagtg gcccgtaaaa gagcttcgtt caaggaagta aaagtatttc ttgaaccaaa
     5881 gtataaggcc atgctcatgc aaatgtgtca tgaagatggc ctgactcagg ctgaagttct
     5941 gaccgcactg ataaaaagtg aagcgcaaaa acgatgtgtg tgatgatggg cttacattct
     6001 tgagtgttca gaagattagt gctagattac tgatcgttta aggaattttg tggctggcca
     6061 cgccgtaagg tggcagggaa ctggttctga tgtggattta caggagccag aaaagcaaaa
     6121 accccgataa tcttcagaaa gtttggcgac tgagaagatt accggggccc acttaaaccg
     6181 tatagccaac aattcagcta tgcggggagt atagttatat gctcggaaaa gttcaagact
     6241 tctttctgtg ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa
     6301 caaacgtatt accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc
     6361 ggaacgctga agttctgcga aaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt
     6421 gatttcgcca ttcatgtggc acatgcccgt tcgaagggac tgcgtcggcg catgccaccg
     6481 gtactgcgtc gccgggctat tgatgcgctg ctgcaggggc tgtgttttca ctatgacccg
     6541 ctggccaacc gtgtccagtg ctccatcacc acactggcca ttgagtgcgg actggcgacg
     6601 gagtctgctg ccggaaaact ctccatcacc cgtgccaccc gggccctgac gttcctgtca
     6661 gagctgggac tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg
     6721 accgacatca cgttcacacc ggctctgttt gctgcccttg atgtgtctga ggatgcagtg
     6781 gcagctgcgc gccgcagccg tgttgaatgg gaaaacaggc tgcgcaaaaa gcaggggctg
     6841 gataccctgg gtatggatga actgatagcg aaagcctggc gttttgtgcg tgagcgtttc
     6901 cgcagttacc agacagagct taagtcacgg ggaataaagc gtgcccgtgc gcgtcgtgat
     6961 gcgggcaggg aacgtcagga tatcgtcacc ctggtgaaac ggcagctgac gcgtgaaatc
     7021 tcggaagggc gcttcactgc taatcgtgag gcggtaaaac gcgaagtgga gcgtcgtgtg
     7081 aaggagcgca tgattctgtc acgtaaccgc aattacagcc ggctggccac agcttccccc
     7141 tgaaagtgac ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc
     7201 cgccggtgca caaaaaatca gcaccacatc ccaaaaacaa cctcatcatc taccttctgg
     7261 tgcatccggt cccccctgtt ttgatacaaa acacgtctca cagacgggca attttgttta
     7321 tccacattaa actgcaaggg atttctctgt aagattacaa ccgttcatgt cataaagcac
     7381 cagccgccag gcttacaggg tgcaatgtat cttttaaaca cccgtttata tctcctttaa
     7441 actacttaat tacattcatt taaaaaaaag acttattcac tgcctgtcct gtggacagac
     7501 agatatgcac ctcccaccgc aagcggcggg ccccaaccgg agccacttta gttacaacac
     7561 acaaaaacaa cctccagaaa aaccccggtc ccgcgcagaa ctgaaaccac aaagcccctc
     7621 cctcataact gaaaaacggc cccgccccgg cccaaagggc cggaacagag tcgcttttaa
     7681 ttatgaatgt tgtaactaag catcatcatc gctgtcagtc ttctggctga acgttctcag
     7741 tacacgctcg taagcggccc tgagggctcg ctaacgcgga gatacgcccc gacttcgggt
     7801 aaaccctcgt cgggaccact ccgaccgcgc acataaactc tctcatggct gaaagcgggt
     7861 atggtctggc agggctgggg gggggtaagg tgaaatccat caatcagtac cggcttacgc
     7921 cgggcttcgg cggttttaca cctgtgctac atgtaacctc cgtataccgc cttacatgcg
     7981 ctgacgcgca ttatttgcct tgttccgtct gaaagtaatc actatgatta aatatgatta
     8041 acgactaatc ggatatgcaa atgaaaaaca ataccgcaca agcaacaaaa gtcattaccg
     8101 cgcatgtgcc attacctatg gctgataaag tcgaccagat ggccgccaga ctggaacgct
     8161 cccggggttg ggttatcaaa caggcgcttt ctgcatggct tgcccaggag gaggagcgta
     8221 atcgcctgac gctggaagcc ctggacgatg tgacatccgg acaggttatt gaccatcagg
     8281 ctgtacaggc ctgggcggac agcctcagta ctgacaatcc gttaccggtg ccacgctgat
     8341 ggaactgaag tggagcagta aggcgctttc tgatttggcg cggttatatg attttctggt
     8401 gctagccagt aaacctgctg ccgccagaac ggtacagtcc ctgacacagg caccggtcat
     8461 tctgttaact catccacgta tgggggaaca gttatttcag tttgaaccca gggaggtcag
     8521 acggattttt gctggcgagt acgaaatccg ttacgaactt aatggccaga ctatttatgt
     8581 attgcgcctg tggcacacac gagaaaacag gtagattgtg tgtggggaca acttaacata
     8641 gcctgcagat attaattctg gaacattatt atcaggccag atatttaaat aatcttgtag
     8701 gagataaaat gaattacgat gaaataacga aaattaccgc tgaacgtatt agcgactaca
     8761 tgactgaagc agttaataca gactctatag ccgttgcgga aatgtttcat aacgcagcat
     8821 ggggagtgcg aacattgtgg tttgaactcg tcacaaaaat agatatagat atacataaaa
     8881 agaacagata tgcgagttat gatcttgatc gataggaatt taaatcccaa aaagattaaa
     8941 aaaacaccca aaaacggatg tttattcaac actgcctgcg tgccatgagt gcaccgccgt
     9001 caattaaatg gatctatttc atcctgacca gcatagtgct ccagacggct catcggcacc
     9061 ggcggttttt tcaggtaaga gccgaagtac accgccacat gtgtggcgtt atccatcacc
     9121 cgggatacgt tgacattcca gccccgccgg taatgactgt ccaggaagcg attccagtcc
     9181 cgtttactgc ttccttctgc tgccagcgca tccggtatca ccaggtccgg tgaggtgtac
     9241 tggcaatagc ggacactacc atttgttctt tttttaagca gccatctgat gatatttttc
     9301 cctgaaggct gccggggaga tattccccag acgagagtga cgacgctgac gattgtagaa
     9361 aatctcaatg tattcccgta ttactgagat ggcttcatcc cggtt
//
LOCUS       DAZFBR010000155         9375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFBR010000155 DAZFBR010000000
VERSION     DAZFBR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.155"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            52..1818
                     /gene="adeD"
                     /locus_tag="ACHJZ2_004373"
     CDS             52..1818
                     /gene="adeD"
                     /locus_tag="ACHJZ2_004373"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO4570358.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1864..3255)
                     /gene="uhpT"
                     /locus_tag="ACHJZ2_004374"
     CDS             complement(1864..3255)
                     /gene="uhpT"
                     /locus_tag="ACHJZ2_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO4570359.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3393..4712)
                     /gene="uhpC"
                     /locus_tag="ACHJZ2_004375"
     CDS             complement(3393..4712)
                     /gene="uhpC"
                     /locus_tag="ACHJZ2_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO4570360.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4722..6224)
                     /gene="uhpB"
                     /locus_tag="ACHJZ2_004376"
     CDS             complement(4722..6224)
                     /gene="uhpB"
                     /locus_tag="ACHJZ2_004376"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO4570361.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6224..6814)
                     /gene="uhpA"
                     /locus_tag="ACHJZ2_004377"
     CDS             complement(6224..6814)
                     /gene="uhpA"
                     /locus_tag="ACHJZ2_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO4570362.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6976..8079)
                     /locus_tag="ACHJZ2_004378"
     CDS             complement(6976..8079)
                     /locus_tag="ACHJZ2_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570363.1"
                     /translation="MNSYIKIDQTGSNKFKITDLYREFNGETKCYDKKCRDMDFSGRN
                     VKEFIRLLSDHDQFEKDQISELTVAANALKLEVAKNNYNMKYSFDTQTERRMIELIRE
                     QKDLIPEKYLHQSGIKKLKLHEDEFSSLLVDAERQVLEGSSFVLCCGEKINSTISELL
                     SKKITDLTHPTESFTLSEYFSYDVYEEIFKKVVFTPGECDLVRDSLVQDGIKPEKIAK
                     IISCLSSRTGIRNLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSFVALNGRNY
                     HQHVDFSEYGFTQTLCVLGAVVNNANDTFDVANYIYTTKGENIIHNDAEDDSPYVHSD
                     ESYRHKDDYWARDNDIRNLRRNFSGNITIATYK"
     gene            complement(8687..9169)
                     /locus_tag="ACHJZ2_004379"
     CDS             complement(8687..9169)
                     /locus_tag="ACHJZ2_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570364.1"
                     /translation="MVELVCFNDDDCAQVRELLKQSNGNITDDKIDEITACLSNPQGI
                     TCFYYSPLQGYQSFLKNTSQDVSELLSKSLNGTISSTSGLKKSGNAFFGTGKYLKEID
                     FTINGFSQKMYLMAVNNVGNEVKLQCSCYFTTGSVDEEKSKSDKIDFTFSGAATTADL
                     "
BASE COUNT         2530 a         2464 c         2154 g         2227 t
ORIGIN      
        1 tttctttatg ataaataaca atcaaattaa taaaatcaaa atgagaaaaa tatgaataat
       61 tccattaacc ataaatttca tcacattagc cgggcggaat accaggaatt gttagccgtt
      121 tcccgtggcg acgctgttgc cgattatatt attgataatg tctctattct cgacctgatt
      181 aatggcggag aaatttccgg cccaattgtg attaaagggc gttacattgc cggtgttggc
      241 gcagaataca ctgatgctcc ggctttgcag cggattgatg cccacggcgc aacggcggtg
      301 ccagggttta ttgatgctca cctgcatatt gaatccagca tgatgacgcc ggtcaccttt
      361 gaaaccgcca ccctgcctcg cggcctgaca actgttattt gcgatcctca tgaaatcgtc
      421 aacgtgatgg gcgaagccgg tttcgcctgg tttgcccgct gtgccgaaca ggcaaggcaa
      481 aaccagtact tacaggtcag ctcttgcgta cccgctctgg aaggctgcga tgttaacggt
      541 gccagtttta cccttgaaca gatgctcgcc tggcgggacc atccgcaggt taccggcctt
      601 gcagaaatga tggactaccc tggcgtaatt agcgggcaga atgcgctgct cgataaactg
      661 gatgcatttc gccacctgac gctggacggt cactgcccgg gtttgggtgg taaagaactt
      721 aacgcctata ttgctgcggg tattgaaaac tgccacgaaa gttatcagct ggaagaagga
      781 cgccggaaat tacaactcgg catgtcgttg atgatccgcg aagggtccgc tgcccgcaat
      841 ctcaacgcac tggcaccgtt gatcaacgaa tttaacagcc cgcaatgcat gctctgtact
      901 gatgaccgta acccgtggga gatcgcccat gaaggacaca tcgatgcctt aattcgccgc
      961 ctgatcgaac aacacaatgt gccgctgcat gtggcatatc gcgtcgccag ctggtcgacg
     1021 gcgcgccact ttggtctgaa tcacctcggc ttactggcac ctggtaagca ggctgatatc
     1081 gtcctgttga gcgatgcgcg taaggtcacg gtgcagcagg tactggtgaa aggcgaaccg
     1141 attgacgcgc aaaccttaca ggcggaagag tcggcgaaac tggcacaatc cgctccgcca
     1201 tatggcaaca ccattgcccg ccagccagtt tccgccagcg actttgccct gcaatttacg
     1261 cccggaaaac gctatcgggt cattgacgtc atccataacg aattgattac gcactcccac
     1321 tccagcgtct acagcgaaaa tggttttgag cgcaatgatg tgtgctttat tgccgtactt
     1381 gagcgttacg ggcaacgtct ggctccggct tgtggtttgc tcggcggctt tggtctgaat
     1441 gaaggcgcgc tggctgcgac ggtcagccat gacagccata atattgtggt gatcggtcgc
     1501 agtgccgaag agatggcgct agcggtcaat caggtgattc aggatggcgg cgggttgtgc
     1561 gtggtacgta acggccaggt ccaaagccat ctgccgttac ccattgccgg gctgatgagc
     1621 accgacacgg cgcagtcgct ggcggagcaa attgacgcct tgaaagccgc cgcccgtgaa
     1681 tgcggtccgt tacccgatga gccgtttatt cagatggcgt ttctttccct gccagtgatc
     1741 cccgcgctga aactaaccag ccaggggctg tttgatggcg agaagtttgc cttcaccacg
     1801 ctggaagtca ctgaataata aaaaaagccc ggcgtcatgc cgggcaaaag tcaccagtta
     1861 cgtttatgcc actgtcaact gctgaatttt tttctcgcgg cggattttgc gttcttccat
     1921 caccgccact atcgccatca ggcagataca accaatcgcg gcgatatcca gcgcagcgaa
     1981 ggtgcctgcc cagccggtaa ggccgaatac tggcgtccca tcggcaatca ttcccagacc
     2041 taacttggca aagctgtcac caatcaggta agcgaaggtg cctttaatac catcggcagc
     2101 gccaatcgct tttttaggta caaagccaac agcagccaca ccaatcaaca attgcgggcc
     2161 aaagaccagg aaacccaacg caaagagaga agccagatag atatattcgt tgctggcatg
     2221 ttggtacaca ccgagcgtgg cgataatcag cgccagcgcg atgcaggcca ccaggccacg
     2281 gcgaccgttc gctaggtcag agagccagcc ccacagcagc gtaccgacca gcgcaccagc
     2341 ttcaaacagc gtaaagccct gaatcgccac cgctttagag agtttcagtt cctggaacgc
     2401 gtacacagtt gaccactggt cgataccaat acgcaccaca tagaggaaaa tgttggcgaa
     2461 gcacagcagc cagatcactt tgtttttcag cacatactca acaaagatct gccacttggt
     2521 catatcggta gattctgtct ctttgtcctc ttcgctgatc tcctcgccga acagctcttc
     2581 agctttgccg aggccataag attccgggga gtcgctgccg taacgcaggc cgataaaacc
     2641 gacaatcagc gcgataatcg acgggaagat aaacatgccg atgacatggc catcgaacag
     2701 gtaatttgcg ccgaacagtg ccacaccagc tgcgcctgca ccgccaaggt tgtgagaaat
     2761 gttccagaaa ccgaggaacg tcccgcgttt acgacgcggc gtccatttgg tgatggtgga
     2821 gtaactgcac gaacccccgg tactctggaa aaagccactt aaggcgtaga aggcaatcat
     2881 caggaacagg ctaaccgagc cgctgcccat actggcgcta aagcccagca tacaaatggc
     2941 agacaggatc agcatgaacg gcaggaattg tttggtgttt ttgccgtcgg cgtagtagga
     3001 aaccagcgtt ttacccacgc cataagtgat ggagaaaccc aggccgatca tccccagctg
     3061 cgtcatgctc aacccgtagg tcgaaatcat atcgttctgc gcgatgttaa agttcttgcg
     3121 aatcaggtac atcgtcaggt agccgataaa gaccaccagg taggattgca tgaacggttt
     3181 gaaccacatt ttgcgccgca cttcgagcgg aaggtccagg gtcggcttgc ggacctggtt
     3241 taagaaagcc agcatgggtt actcctgaaa tgaatacctg cgtgaggcat gcattgtaaa
     3301 aatcagcagc aaatcaggcc tgagatagcg tccaggcaaa acctggaaaa tttcttagtt
     3361 ttgccggata tgaagtgaaa aggtgagatg catcacgctt cgcgcggtgt ctgggcgttc
     3421 aaaaagggca gtaacagcag tgcggaaatc ccggcggcaa tggcgataac cacaaaaaat
     3481 ccgctccagt gccaggtatc gagcactttc gccagcggcc aaccagcaag cgacgccccc
     3541 agataagcaa acaagccgac aaaccccgtc gccgcccctg ccgcctcttt gtgggaacat
     3601 tctgccgccg ccatgccgat taacatctgc gggccaaaga caaaaaaacc aatggtgaag
     3661 aagcaggttg cctgcatcac gtagctggca aatggcatca gccacagcga gccgactgaa
     3721 agcaaaattc cggcagcgaa aatcaaattc atcggcccgc ggttgccgtt aaacagtttg
     3781 tccgagcccc aacccgctac cagcgcaccg ataaatccgc ccagttcaaa catcgtcact
     3841 gccgtattcg ccgtgaccag atcgacgccc agcgtctcgg acatatacaa attgccccag
     3901 tcgttgatcg ccgcccgaac cacatagacc agcacatagc aaaacgaaag cagccagata
     3961 tacggattca acaacacata cttggtgagg atctctttac gcgtcagccc tgccccttct
     4021 tgttgttgag caatctccag cgcgtcgtgt cgccattcac cgaccgctgg taaacctaac
     4081 gcctgcgggc gatcgcgcag ccgccagcag agaaaaatcc ccacgacgat cgccatacaa
     4141 ccagcaatca tcatcccggc acgccagccg taatgcagcg cagctgctgc catcacaatg
     4201 ggaatgagcg caccgccaac gttatgcgcc gtgttccata atgcccacca accaccgcgt
     4261 tcggtacgag aataccaggc cgttaacaga cgcgcacaca ccggtgaacc ccagccctgg
     4321 aaaaaggcgt tcagcaccca gagcacggca aacgcccata gcgacgtcga gaagccaaac
     4381 agaatgttga tgatgcccgt ggcgataagc cctatcccca taaaataacg ggcatttgag
     4441 cggtcgctga caatgccgga gacaaacttc gacacgccgt aggtaatgta aaacagggtc
     4501 gctaacaggc cgatatcgct acggctgagc acgccattag caaggatttc tggtacggcg
     4561 gcgttaaaac ttttacgcgt gaagtaaaac agcgcgtaac ccagccagat ggtcagcaga
     4621 atatgccgac gccagtagcg ataacgggca tcaatttcat gtttatcagt cattaatggc
     4681 gcatcggcag gcgctttcag aaacggcaac atcatcaaac cttagacata gcgttgaggt
     4741 agagaaacgc tgacacgcgt gccgtgcaga caggaaatgg tcaaggtgcc gcccagcgcc
     4801 gttacgcgct cgcgcattcc ggtgaggcca aaaccttgtt gcccggaacc cggcggtaaa
     4861 ccgctgccat cgtcttcaat caccagcatc aaccgttcat cctgcagcca gccttgcagg
     4921 gtgaccgcgc tggcatcagc atgtttcaca atgttattca gcccttcctg gcagacacga
     4981 aacagcgtca cgcgctggtt ttcgcttaac gctgactctt cgattcgcca ttcgagatgg
     5041 ctgacaatgc cgcgcccttc cagctccatt tcccgcatca gtgagcggat ggcctgctct
     5101 agggtgagat catccaactg gcgcggacgt aaccgcccta acaaacggcg caccgcgtcg
     5161 tacacgccca gcgacagttg ttcgatgagc tgcccgctct gcttcacact ggcgttatct
     5221 gccgccagtc gctggacaat gcccgcttga gtacgaatgg cagtgatggt ctggccgata
     5281 tcatcatgca gctcacgcgc cacatcacgg cgcacgctct cttcagtttc taacaaacgt
     5341 tcagcgagat gctgattgcg cgccagttcc ttttgcagcg actggttaag ttcacgcaac
     5401 cgctggatgc cagcgcccag taacaaccct gtcagacttt gcaccagcag cgagagcaat
     5461 aaatccacag gatgatcgcg ccaggtttga ctggcgatca gcgcgatggc gttcatcaat
     5521 gtcgctatca gcgccccttg ccagccgtag tgccaggcca gcgcgataat cggcagcgcc
     5581 aggcagaatg gcgtaaagcg cgacagttcg tccggcaagc ccaactggag ccagagactg
     5641 ataacaaaca gcagcaagta ccagaccaga tgccgcccgc gccagttgat cggttgagaa
     5701 actaacgacg gcccgagcgg cagccaggtg ttattggcga gataatgcca gaacaccaga
     5761 catatcgggg ccagcgtcag gccgccagtt aaagttaaca gcagcgcatt ccacgactct
     5821 ttgccgtgcc aaagccaggg cagcgactgc aacaacgccg ccgccgttaa cgccgcaccc
     5881 tgtaacagca aggtgcgcca gtcacgttga tggcgatagc gcgagatcag cgccacgggc
     5941 agtaacgtca gtaaactacc gatcatcaat aaggagaaat gggttaaacc gaccgcctgc
     6001 gttagccagt aaatcagcag ccactccgct cccagcagca ccggccagta tccgcgcggg
     6061 cattgcagca ttagccctag ccgcagacca aacggaaata acagcaccgc catatcaggg
     6121 cgctcaacca ggtgcaggct gatactccac aggcaaaacc atgcggcaga gaagataaaa
     6181 aagcaggcaa taacggtaat taagcgggag aacaacgtct tcatcaccag ccatcaaaca
     6241 tacggcgcgc cagttcaacg tcgttactga cgcccagttt ttccatcaga ttggcgcgat
     6301 ggacgtgtac cgttttcggt gacaagccca gttcggcggc aatctctttc accgccattc
     6361 cttgcgccag tttttccgcc acctgccgtt cacgtttggt tagtgggtcc tggcgaccgg
     6421 atgccagttt aatggcaata tccggcgtca gataacagcc gcctgtggca accgtatgca
     6481 ccgcagcaat cagttcgtca gggctacaac gcttggagag aaagccacgc gcccccgcgt
     6541 taagcgcctg ctcaaccagc gccggactgt catgaacgga gagcataatt gtcgccatac
     6601 ctttcggcag ctggcttagc agctccagac cggagatatc gggcatggag atatcgcaaa
     6661 tacacacctg cacaccgcgc cccggcagcc ccgccagcgc ctcgcgcccc gaaccaaact
     6721 cggcaactac ttgcaaatca ggttccagcc ccagcaactg cgcaaagccg gagcggacga
     6781 tgaggtgatc gtctataagg gcaacggtga tcatgattgg gtcctggcgg ataaaaaacg
     6841 cgcatacctt aacgataagc gcgatgttgt tcaagttgga tatggggtat gtattggctt
     6901 aatgatgttg agcttatcgt caaaacgctt acctgagagg taagcgtttg gtgcgtgtta
     6961 ttgagaaaaa gcgaattatt tgtaggttgc aatcgtaata ttgcctgaga aatttctcct
     7021 tagattgcgg atatcattat cccttgccca ataatcatct ttgtgtctat aactctcatc
     7081 ggaatgtaca tagggagaat catcttcagc gtcgttatgg ataatatttt cgcctttagt
     7141 ggtatagata taattagcaa catcaaaggt gtcattcgcg ttatttacga ctgcgcctaa
     7201 gacgcaaagg gtttgagtga atccgtattc tgagaaatcg acatgctgat ggtaatttct
     7261 accattcaaa gcaacaaaac tcggacaaac atctattgta tcgccaacag acgtagcggc
     7321 gcgacgcaca tcaagtgcag cgttatcgtt agcaacatga aatgcttgtg aacaggcata
     7381 aaacaaattt ctaatcccgg tacgactgga taaacaagaa ataatttttg ctattttttc
     7441 tggctttata ccatcctgta ctaaagaatc tctcaccaga tcacattcac caggtgtgaa
     7501 aacaactttc ttgaatattt cctcataaac atcataagaa aaatattcag agagagtgaa
     7561 agattcggtg ggatgtgtta aatctgtaat ttttttgcta agtaattcac ttatagtgct
     7621 gtttattttt tcaccgcaac ataaaacaaa tgaacttccc tccagaactt gcctttctgc
     7681 atctacaagg agtgagctaa attcatcctc atgaagctta agttttttta tacctgattg
     7741 atgtaaatat ttttctggaa ttaaatcttt ttgttccctt ataagctcta tcattcggcg
     7801 ttcggtttgc gtatcaaaag agtatttcat gttatagtta tttttagcaa cttccagctt
     7861 aagagcattt gcagcaactg ttaattctga gatttgatct ttttcaaatt gatcgtgatc
     7921 tgataataac cttataaact cttttacatt tcgaccacta aaatccatat ccctacactt
     7981 tttatcataa cactttgttt cgccattgaa ctcacgatac agatcagtga ttttaaattt
     8041 attacttccg gtctgatcaa tttttatata gctattcatg cctgtaacgg caatctatgt
     8101 cttataattc gcattctccg attcaggcgt aacatgaaaa taaggaacca atttatcact
     8161 aatatcatgc cgatagatat gaaatgaatc taaaagatat aataaaaaac ttcgaaaaat
     8221 gtcacgaaaa gtgtcatctt taaaccttgc cctataatta tcgagcaggc cgctttgttc
     8281 tatcatatta aatttataaa tattatcctc gagacttttg tcgatactct cccttagact
     8341 ctgattttga aactggctat tatcattctc tgtttccgtt ggatatgaat aactcgtcac
     8401 actaaccata gtaacctcgc aatatgacac tttgcatatt ttgacattgt tagaagtatc
     8461 aatggctagc atgataaata tctcaatgct aaccgctata tttcatttat ttcaataata
     8521 agactatagt gccgttaatt tttatcgatt tccccaatgc aattgcatca ctaatactac
     8581 ttattcacta tcaaaaatcg ctcatttttt caaaaagact gtttacacac agacggttaa
     8641 caatacgtgt tcaccttaat gataaacgcg ctttatcaag aaaaaatcat aaatcagcag
     8701 ttgttgcggc accggaaaat gtaaaatcga ttttatcgct tttggatttt tcttcgtcga
     8761 cactaccggt agtaaaataa caagagcact gaagcttcac ttcatttcca acattattaa
     8821 ccgccatcaa atacatcttc tgactaaacc catttatagt aaaatcaatt tctttaagat
     8881 acttcccagt cccaaaaaag gcatttcctg acttttttaa acccgatgtt gatgaaatgg
     8941 taccattcaa actctttgag agtaactcgc taacatcttg agatgtattt tttaaaaaac
     9001 tctgatagcc ttgcaaagga gaataataaa aacatgtaat cccctgaggg ttggataagc
     9061 aagcggttat ttcatcaatc ttatcatccg taatattacc gtttgattgt tttaataatt
     9121 cccttacctg agcacaatca tcatcattaa agcaaaccag ttcaaccatt tgttcaagtg
     9181 tgttttgact attctttgga tctttttcaa ttatttcaat tttgtctgta aatattttaa
     9241 gaatatcttc acttaactca acaccgcagc aagttatttt agtagcaccc ttttgttgac
     9301 tttgttcttt tatagttcaa tgagcatcca taatcatgga atcaaaaaaa acatcagttc
     9361 ttttaaaact ttcct
//
LOCUS       DAZFBR010000156         9252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFBR010000156 DAZFBR010000000
VERSION     DAZFBR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.156"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            159..521
                     /locus_tag="ACHJZ2_004380"
     CDS             159..521
                     /locus_tag="ACHJZ2_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570365.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(700..1158)
                     /gene="ybbJ"
                     /locus_tag="ACHJZ2_004381"
     CDS             complement(700..1158)
                     /gene="ybbJ"
                     /locus_tag="ACHJZ2_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO4570366.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1155..2072)
                     /gene="qmcA"
                     /locus_tag="ACHJZ2_004382"
     CDS             complement(1155..2072)
                     /gene="qmcA"
                     /locus_tag="ACHJZ2_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO4570367.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2218..2895
                     /gene="fetA"
                     /locus_tag="ACHJZ2_004383"
     CDS             2218..2895
                     /gene="fetA"
                     /locus_tag="ACHJZ2_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO4570368.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2882..3661
                     /gene="fetB"
                     /locus_tag="ACHJZ2_004384"
     CDS             2882..3661
                     /gene="fetB"
                     /locus_tag="ACHJZ2_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO4570369.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3724..4578)
                     /gene="cnoX"
                     /locus_tag="ACHJZ2_004385"
     CDS             complement(3724..4578)
                     /gene="cnoX"
                     /locus_tag="ACHJZ2_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO4570370.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4639..5448)
                     /gene="ybbO"
                     /locus_tag="ACHJZ2_004386"
     CDS             complement(4639..5448)
                     /gene="ybbO"
                     /locus_tag="ACHJZ2_004386"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO4570371.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5438..6061)
                     /gene="tesA"
                     /locus_tag="ACHJZ2_004387"
     CDS             complement(5438..6061)
                     /gene="tesA"
                     /locus_tag="ACHJZ2_004387"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO4570372.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6032..6718
                     /gene="ybbA"
                     /locus_tag="ACHJZ2_004388"
     CDS             6032..6718
                     /gene="ybbA"
                     /locus_tag="ACHJZ2_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO4570373.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6715..9129
                     /gene="ybbP"
                     /locus_tag="ACHJZ2_004389"
     CDS             6715..9129
                     /gene="ybbP"
                     /locus_tag="ACHJZ2_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO4570374.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2108 a         2375 c         2544 g         2225 t
ORIGIN      
        1 gtgctctttc aatgtaaaaa agagtaaaga cttgttttga taagcgaaat aaattctgtt
       61 tctggactaa acgtgaagcg gttcctgatg tgtgctaaaa tgcgtttctt tgataaatct
      121 caatttttaa gaggtttacg ccaggcagga aaatttaaat gaatgaattt gagaagattt
      181 ttaatgaaat gaatctggac cgggcattgc taccgatcct ttttcgttcg aaccggtcga
      241 cagtgtggaa gtatttaagc ggcgatagta cggctcctgc ctctgccatg tcgctgatta
      301 tgctgttgca gttgatacag aaacgaaacc ccgatctgtt agccgaatgg ttaacgcttt
      361 ctgatttcac cattccgcca gaagtttacc tggaccagcc tgattactgg aaagggtggg
      421 tgtatacgca acacaaggtc aataaaaacg tcctggaata tctaaaaaaa cactatccgg
      481 atgaagacca aaaaagtatg agtaaaggcc gggaagagta aattgccaat gccagttaac
      541 atttaacatg gcggaggcgg cctacggttt tggcgtgata tgacgcgccc gcgtcgcatc
      601 aggcgtcggt gcacgactgc cggatgcggc gtaaacgcct tatccggcct acgattcggg
      661 cgcagacttc cgggcatgat aagacgcgtc agcgtcgcat caggcgatga ccgcacggat
      721 gatcagcgtt atcccttcta tcgcaatgac ttcaacatgc gtacctgcgc cgagatcctc
      781 gctggcgctg acaggccatg aactgtcacc gacgcgcata tgaccgcgcc cgttgaccag
      841 cggagattcc agcacaaaac gtcggccaat cagctgctgc ccgcgctggt ttaaatgact
      901 gtcgctgtgc ttttgttcgc gcacccgccg cgacaaccat ttccaccaca gccaggcggc
      961 gagcagcgtc aggacggcaa acatcacccc ttgccactcc caacccagcg gcaccagcca
     1021 gaccaccagg ccagtaatca ctgctgccac gccactccac aacaaataac catttccgcc
     1081 cagcatctcg gctgccagca gcaaaccgcc gagactgagc cagaaaatat gtggatgaac
     1141 gactattaac tccatcatgg ctgagtccgc ttgttggcgc tgtctttcac cagctcggca
     1201 atcccggcaa tcgaccccat caggctgctg gcctctaatg gcatcatcac tactttgctg
     1261 ttactggagg aaccgatctg ctgtaacgct tcggtgtatt tctgcgctac gaagtagttc
     1321 accgcctgaa tatcaccgga ggcgatggct tcagacacca ttttggtggc gcgggcttct
     1381 gcttcagcgg aacgttcacg cgcttcagcc tgtaaaaacg ccgactgacg ttcgccttcc
     1441 gctttcagga tttgcgactg tttttcacct tcggctttga ggatttccgc ctgacggatc
     1501 ccttccgctt caagaatgta agcgcgtttg gtacgttccg ctttcatctg cgcgttcatt
     1561 gaagagataa gctctgccgg tgggcgcacg tcgcgaattt caatacgggt gactttaatc
     1621 ccccacgggt tggtggcctc atcgacaata cgcagcaggc gtgagttgat gctgtcgcgc
     1681 tgagagagca tttcgtcaag ttccattgaa cccaacacgg tacggatgtt agtcatggtc
     1741 aggttgatga tcgccagctc cagattgctg acttcataag ccgcgcgtgg cgcgtcaatc
     1801 acctgaataa aacagactgc gtcgatggta acgttggcgt tatctttcga gataacttcc
     1861 tgggaaggga tatcgagcac ttgctccatc atattgatct tgcgaccaat gcgatccata
     1921 aacggcacca ccagactgag ccccggctgt aacgttttgg tatagcgacc aaaacgttct
     1981 accgtccatt gatagccctg cggcacgatt ttgacgcccg cgccgacaat gaccagcgcg
     2041 acaaaaatga gaatcgggat aaagataagc atcggaaaaa cctcctgttg taccgtccat
     2101 aatcagcaaa attgctgctt gattaaacaa attatacctg attactgaaa gagagttccc
     2161 ccttattcct gcgaaggata aactgttttt agtaaaaatc agaaaaaggg aacagcgatg
     2221 caggaaaata gtcctttgct tcagctacaa aacgtaggat atctggcggg tgatgcgaag
     2281 attcttaata acatcaattt ttcgctgcgt gctggcgaat ttaagttaat taccggtcct
     2341 tctggttgtg gcaaaagtac gctgctaaaa atagttgctt cattgatcag cccaaccagc
     2401 ggaacgttac tgtttgaagg tgaggatgtc agcacactaa agccagaaat ctaccgtcag
     2461 caagtctctt actgcgccca gacgccgacg ctgtttggcg atacggtata cgataatctg
     2521 atctttccct ggcagatccg taaccagcag cctgacccgg ccatttttct cgattttctt
     2581 gaacgcttcg ccttgccgga cagcattttg acgaagaata tcgccgagct atctggtggt
     2641 gaaaaacaac gcatctcatt gattcgtaac ctgcaattta tgccgaaggt tttattgctg
     2701 gatgaaatca ccagtgcgct ggatgaaagt aataaacata acgttaatga gatgatccat
     2761 cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga cacacgataa agacgaaatt
     2821 aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg gagaaatgca ggaagcacgc
     2881 tatgaactcg cataatatta ctaacgaatc attagcactg gcattaatgc tggtagtggt
     2941 ggcaatctta attagccata aagaaaaact ggcgctggag aaagatattc tctggagcgt
     3001 cgggcgcgcg ataattcagc tgattattgt tggctatgtg ctgaagtata ttttcagcgt
     3061 ggatgatgcc agcctgacat tattgatggt gttatttatc tgctttaatg cggcgtggaa
     3121 cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc tcatcgttta ttgccattac
     3181 ggtcggggcg ggaattaccc tggcggtgct gattctctca gggtcgattg aatttatccc
     3241 gatgcaggtg atccctatcg ccgggatgat tgccggtaac gccatggtag cggtggggtt
     3301 gtgttacaac aatttagggc aacgggtcat tagcgaacag caacagattc aggagaaact
     3361 gagtcttggc gcgacgccga agcaggcttc agcgatattg attcgcgaca gtattcgcgc
     3421 ggctttaatt ccgacggtcg attcagcaaa aacggttggc ttagtgagtt taccaggaat
     3481 gatgtccggg ctgatatttg ccgggattga tccggtgaag gcgattaaat atcagattat
     3541 ggtgaccttt atgctgctct caaccgccag cttgtcgacc ataattgcct gctatttaac
     3601 ctatcgtaag ttttataatt cgcgccacca gttggtggtg acgcaattga agaagaaatg
     3661 atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg ttcatgtgcc gatcaacgga
     3721 atatcaatac aacaatgcat acagctggcg gcgatacttc gacgccagtg catcacccgt
     3781 acccagcgca gcgaggatct cctggaacgt tttacgcgtc tgaccgtctg cggcggtgag
     3841 atctttacgc agatgcccga acagcaactc cagcgcctct tcattgcgcc caacctgatg
     3901 cagttgcagc gccagttgcg tcgccagtgc ggcatcttct ggattctccg ccacctgctg
     3961 ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc agcagttcga tttgcgccac
     4021 cagcccctgg tagcgggtgt cctgatcctg caacggaatg gttttcagca ccgcttccgc
     4081 atcttcagaa cggttcagcg caatcagcgt ttctgccagc agcaggccga tctccccgtt
     4141 ctgattcgac aactgccagg cgtctttcag caatggcagg gcatcggtgt aattgccttc
     4201 ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct tcgcgcggca gcactttatc
     4261 cagcagggcg cggatcgcct cttccggttg cggcccctgg aagccatcta ccggttgccc
     4321 gttctggaac agatacacgg tcggaatcgc gcgcagacca aactgggcgg caatcatctg
     4381 ctccgcgtcg cagtccagct tcgccagaat aaattgcccg ttgtactgcg ccgcaaggct
     4441 ttccagaatt ggggttaact gcaaacagtg ctggctacgt tcagaccaaa aatagaacag
     4501 caccggagtg gtcatcgact gttcaagaac ctgttgcagg ttagattcgt taatgttgac
     4561 aatattttct acggacatgg agtcgctctc tgttgtcgat attttctttg acatgggggc
     4621 ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc atcacgcgcc ccggcagcag
     4681 gcgcttaagc accattaccg cccaggtcac cagcgttacc ggatagcgca tcttcggctt
     4741 ctcgctaata aaagcatggc gtactttgtc caccaccgct tccggtccca acgtaaagcg
     4801 ggcggcgatg ccgggatttt cgactggttt atcactttgc gtctggttga cgttgtcggt
     4861 gaagcgagta cgaatgggac cgggttcgat caggctgact ttaattccgc tgcggcgcag
     4921 ctccatgcgc agcgcgtctg accacgcctc cagcgcatat ttactggccg cgtaagcgcc
     4981 acgacccggt gtggagatta atcccatcac cgatgaggtc atcacaatac gcccttcacc
     5041 gtgcggcaac atcgcgggta acaggcgcat ggtgagctgg tgtgcgccga aaaagttggc
     5101 ggaaaactgc tgttccatct gcgcacggct gatggtggaa agggggccat acatgccgaa
     5161 tccggcattg ttaaagatcc catacagaca attatcggtc agggcgatca cctcgtcggc
     5221 tgcgcgatca acactttctg gtgaatccag atcgatcaac acgccggtaa atcccatgct
     5281 gttcatgcgc tcaacatcat ccggtttccg gcaacctgcc agcacatgaa aaccctggcg
     5341 ttttaattcg agcgcgcttt ccaggccaat tccactggaa catccggtaa ttaagaccga
     5401 tttttgcata actttacctg tcaggatctc cgttgcttta tgagtcatga tttactaaag
     5461 gctgcaactg cttcgccatc cagtcggcaa taaacggctg ggcgtcgcgg ttgggatgaa
     5521 taccgtcatc ctgcatccat tgtggcttga ggtagacctc ttccattaaa aagggcagca
     5581 gcggaacatc aaactctttg gcgagtttgg ggtaaatggc gctaaaggct tcattataac
     5641 ggcgaccata gtttgcaggc agacgtattt gcattaacaa tggttcagcg ttggcggctt
     5701 tgacatcctg caaaatctgg cgcagcgttt gctcggtttg ctgtggctga aaaccacgca
     5761 aaccgtcatt gccgcccagt tcaaccagca cccaacgcgg ctgatgctgt ttcagcagag
     5821 ccggaaggcg cgccagccct tgttgcgagg tgtcgccgct gatgctggca ttgactaccg
     5881 acgttttact ctgccactta tcattcaaca aggcaggcca ggccgcgctg gcagacattc
     5941 gatacccggc gctcaggcta tcacccagaa tcaataacgt gtccgctgcg gcagcacgga
     6001 aggttaacag gaccagaaac aggaagggca aatgccagcg gaaaacattg ttgaagttca
     6061 tcatcttaag aagtccgtcg gtcaggggga gcatgaactc tccatcctca ccggagttga
     6121 gctggttgtc aaacgtggcg agaccatcgc actggtgggc gagtcgggat cgggtaagtc
     6181 aaccttgctg gcgatcctcg ccgggctgga tgacggcagc agtggcgaag tgagtctggt
     6241 gggacaaccg ctacataata tggacgaaga agcgcgggca aagttgcgcg cgaagcacgt
     6301 cggctttgtt tttcagtcat ttatgttaat tcctaccctt aacgcgctgg aaaacgtcga
     6361 gcttccggcg ctgctgcgcg gtgagagtag cgcggaaagt cgtaacggag cgaaagcgtt
     6421 gctcgaacag ttagggctgg gtaaacgtct ggatcatctt ccggcacagc tttccggcgg
     6481 tgaacagcaa cgagtggcgc tggcacgagc ctttaacggt cgacctgatg tgctgtttgc
     6541 cgacgaaccc accggtaacc ttgaccgcca gacgggcgat aaaattgccg acctgctgtt
     6601 ttccctcaac cgtgagcatg gcaccacgtt gattatggtg acccacgacc tgcaactggc
     6661 ggcacgctgc gaccgctgct tacggctggt gaacgggcag ttgcaggagg aagcatgatt
     6721 gcacgctggt tctggcgcga atggcgctcg ccgtcgctat taattgtctg gctggcgcta
     6781 agcctggcgg tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat ggagaagggc
     6841 ttaagccaac aaagccggga gtttatggcg ggcgatcggg cgttgcgcag ttcgcgcgaa
     6901 gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg caagcagctg
     6961 actttcgcca ccatgacatt tgcaggcgac acaccgcagc tggcgaacgt taaagcggtg
     7021 gatgatatct acccgatgta tggcgatctg caaaccaatc cccctggcct gaaaccgcag
     7081 gcgggcagcg tattgctggc cccacgcctg atggcactgc ttaacctgaa aacgggcgac
     7141 accattgacg tgggcgatgc caccttgcgg attgccggag aagtgattca ggaaccggat
     7201 tccggtttta accccttcca gatggctccg cgtctgatga tgaatctggc ggatgtcgat
     7261 aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc gctataaatt cggcggcagc
     7321 gagaaccagc tcgacagcta tgagaaatgg ttgttacctc agcttaaacc cgaacaacgc
     7381 tggtacggtc tggaacagga cgaaggcgcg ctggggcgat cgatggaacg ctcgcaacag
     7441 ttcctgctgc tttcggcact tctgaccttg ctgctggcag tggcagcagt ggcggtagcg
     7501 atgaatcatt actgtcgcag tcgctacgat ctggtggcga tcctcaaaac gctgggggca
     7561 gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct gacgctttca
     7621 gccgttaccg gtggggcgat aggcctgttg ttcgaaaacg tgttgatggt gctgctcaag
     7681 cccgttctgc ctgctgcact accgccagcc agcctctggc catggctatg ggcgctgggc
     7741 accatgacgg tcatctcgct gctggtgggg ctacgaccat accgcttgtt gctggcaacg
     7801 cagcctttac gcgtattacg taatgatgtg gttgcgaacg tctggccgct gaagttttat
     7861 ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg tggcagcatg
     7921 ctgctttggg cggtgctggc gggcgcggta gtactggctt tgctgtgcgg tgtgctgggc
     7981 tggatgctgc tgaatgtact tcgccgcatg acgctgaaat cgctgcctct gcgcctggcg
     8041 gttagccgcc tgttacgtca gccgtggtca acgttaagcc agctttcggc attttcgctc
     8101 tcctttatgc tgctggcact gctactggtg ctgcgtggcg atctgctcga ccgctggcag
     8161 cagcagttac cgccagaaag cccgaactac tttttaatca acatcgccac agaacaggtt
     8221 acgccgctaa aagcgttcct cgcggaacat cagatagtcc cggaatcgtt ttatccggtg
     8281 gtgcgagcgc ggctgacggc gattaacggc aagccgacag aaggcaatga agatgaggcg
     8341 ctcaaccgcg aactcaatct tacctggcaa aatacgcggc ccgatcataa tccgattgtc
     8401 gccggtaact ggccgccaaa agctgatgaa gtttcgatgg aagaggggct ggcgaaacgc
     8461 ttaaacgtta ccctcggcga taccgtgact tttatgggcg atacccagga gttccgcgct
     8521 aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt ctattttatt
     8581 ttccctgaag gggcattaga cgggcaaccg cagagctggc ttaccagttt ccgctgggag
     8641 aatggcaacg gcatgttgac gcaactcaac cgccagttcc ctaccattag cctgttagac
     8701 attggcgcga ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg ggcgctggag
     8761 gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt tgctggcaca ggtgcaggtg
     8821 ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca cactcggtgc ggggaaaaaa
     8881 ctgctgcgta ccacgttgtg gtgtgagttc gccatgcttg ggtttgtttc cggcctggtg
     8941 gccgcaattg gtgcggaaac ggcactggca gtgttgcagg cgaaagtgtt tgattttccg
     9001 tgggagccag actggcgatt gtggattgtt ctgccgtgca gcggtgcgct gctgctgtcg
     9061 cttttcggcg gctggctggg cgcgagactg gttaagggta aggcgctgtt caggcagttt
     9121 gcggggtgat gagagtaaat aaggggcgtt ctggttgaat ggaacgcctt gtttaaatta
     9181 attctacaat atgtttcatt gatgatgtta ttgaattggt gatttcctat ctttctattg
     9241 ctgatattaa tc
//
LOCUS       DAZFBR010000157         9200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFBR010000157 DAZFBR010000000
VERSION     DAZFBR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.157"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(123..446)
                     /locus_tag="ACHJZ2_004390"
     CDS             complement(123..446)
                     /locus_tag="ACHJZ2_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO4570375.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(454..639)
                     /locus_tag="ACHJZ2_004391"
     CDS             complement(454..639)
                     /locus_tag="ACHJZ2_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO4570376.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(636..3275)
                     /locus_tag="ACHJZ2_004392"
     CDS             complement(636..3275)
                     /locus_tag="ACHJZ2_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO4570377.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            3483..4472
                     /gene="ldhA"
                     /locus_tag="ACHJZ2_004393"
     CDS             3483..4472
                     /gene="ldhA"
                     /locus_tag="ACHJZ2_004393"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4570378.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4583..5005
                     /gene="hslJ"
                     /locus_tag="ACHJZ2_004394"
     CDS             4583..5005
                     /gene="hslJ"
                     /locus_tag="ACHJZ2_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO4570379.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5002..5268)
                     /locus_tag="ACHJZ2_004395"
     CDS             complement(5002..5268)
                     /locus_tag="ACHJZ2_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO4570380.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5542..9066
                     /gene="nifJ"
                     /locus_tag="ACHJZ2_004396"
     CDS             5542..9066
                     /gene="nifJ"
                     /locus_tag="ACHJZ2_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO4570381.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLTVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2292 a         2407 c         2262 g         2239 t
ORIGIN      
        1 tttaagggaa ggatatataa ggtttttgta gaaataccca cggtaattaa gcatgctaac
       61 gtcagtttaa atactgtaaa agatcattac attaatagat acattttgta gccaactata
      121 atttatttgc gcaaccattt accattaact ccctgaacat attgccccga cttcgcgcga
      181 gcaacaagct tttgtccagc cagtttggct atctctttag tggatacatt attttgcttt
      241 gccagttgtt ggtaactatg gttacgagct tcattaatat cttttactaa tgcctgtgtt
      301 tcggcatccg tttgtagcgc caccaggtaa ccgttaagcg tttcacctac ccgcccctga
      361 cttctggctt cgttcagggt taaagccatt gcactgtcgc tcatcaggcc aatggcccat
      421 acagctatga gtaatgttct tttcatcatc acctcaaaat agatcgctac gggttttgag
      481 caggtcttca acgtccttat ccgctttaat gatgatttca tgctcaattt taacgttcat
      541 attaatggtg attggctctt tcggcgcagc cacttcaatg cgaggcgtgc aaccaaccaa
      601 cataaaagag gacgcaaacg cggctatcac tattttcatt gtttttcctc acactctttt
      661 cctgatgggc agccagtttc cggtagctgg gcattctgct ccagccatac ctggagatta
      721 tcgccaaagc gtaaactgcg ccataacgta aaaatattct cttcatggtg ataattaagg
      781 tttaccgtac cgcttttacc atcgacgcga ctggtgcccg aaatgttggc ctgcatggtt
      841 aacacaccga gattatctaa attaatcctt gttgatgagc gactgatttc catataacgc
      901 aaccagttga tggcagttcc agcggtcata ttgtctttgg ccaccgcatc tgcagtgtct
      961 ttatccagac gcagcgtcat cggccccgcg tttgccaacc agccatcttt gacaatccat
     1021 ttttcattgt tcagccatag tggcaacgcc ccactaaatg ccccagacat agcaaactgt
     1081 ttcggattaa ccgcgctaac cagttcgctg gatgagagat tattcagccg caacagcgcc
     1141 gggtcatgtt gcggcatacg taattgtttc atcagcacgt taccgcctaa cacatcgacg
     1201 ctaacatcag tcaacagcaa tggttcttct tcagtccacg gataacgccc ttgcaaatca
     1261 gccgtaatat ttttcgctgt caccagatta atcacttcgg caattcgcaa cgtaacgggg
     1321 ccgcgagtcc ccagatgcca ggctccatcg gcaaaacgga aaggcaggac aaaatcgaca
     1381 ccgttaacct gattatctgg catccaggca ctaccgcctt tcaacacgcc gtgccctccc
     1441 gcgcggaatc cttgttcagg cgcagcagaa aatgcaacct gagcatacag ttcaccatcg
     1501 cgtaagttca tcttccagtc gggtggcacc agcggctgga atacagtcag tgattgttta
     1561 ggccaccagg cgttaccgcg cagacgaata ccgtcccagc gaccatttac ccgaaccggg
     1621 ccaatctcac cagcatgtaa atcgccttta aagaggaaat acgtcggatc gcgcccatcg
     1681 acgctaaatt ttaaggttga tggcggtaac acactgccgc cagtgaacag cgtttgcccg
     1741 gcgtccagtg acagcgcgcc gctgaaggag ggatgctgtg cgtcacgtac ccagacgacg
     1801 ggcttgtcga gaattaatcg cggcttttct accgtcatcg taccgtattg taactggtcg
     1861 aaaccggtgg agagatcggt cagcgtaatc gtgctgtcat gccactcacc ttttcctgcg
     1921 acatcccagg tggcattcat cggtgtaaaa ctcccttttc cccagtagcg ccactgccag
     1981 cggccagcgt cagggagaaa atcattcgcc agcccatcca tatgcagcac gaaatcgccc
     2041 agttcatttt catgcgcctg caaaatggcc tgcaaacgtc cgtcaacacc acgttgggtg
     2101 acttttacac ccgctaaagg ccagcggatt tcatcgatgt ccagcgaatc gatgactctt
     2161 ccctttgaac gaagtaacgc gcctggctca aaggtcagcg ttgggtcaga cagacttcca
     2221 cttagctgcg caggtaaacg ggcatataaa atgaggtccg cctgtttcgc ttcaccggtc
     2281 agctgcagag gcagctggct gttatccatg cttaattttc ctgggccaaa attaagcacc
     2341 gcgttgccct tacccgcttg cccctgggtc agcacactca gtcgtccgct gaccagagcg
     2401 ttctcaagcc ctgcctgcca gttgtcgact ttgacaccca gtcggccact caaaggaaaa
     2461 cctgcatacg gccagctcca gcgaccatcg cttacggtca attgttgacg agtaatttgc
     2521 cacggcaaat cgagcaacgg atcgccgtta tcccgtgcca gcacgatcaa ttgcccgcta
     2581 ttttcctgcc agtccagctc ggcatccacc agtgacggtt cctgcggcaa gtttaacgtc
     2641 gcagtagcat gcccacttac aggaagtcca tccggcatga gcggcatagt aaattccccc
     2701 accagtttaa ccgacggctg attttcaaac gcgacgacat ccagttcgct gactgtaagt
     2761 tgttgccctt tcagctggcc ttgaaattta actttttcgc cctgataacg cagttgctgg
     2821 atatcagagg ttaatgcgag agagagtttt ccctgccatt cctgccaggg agaaaaaatc
     2881 agtttatcaa tattgatcca ggtgttaggc agcatggact gccattgcgc gagggttttt
     2941 ggagcagctg gcgattgctc cgtctgcggc aatttcgcca gacaagcaga atcaagttct
     3001 accgtgccga cgttcaataa ccagcggctg ggatgtgaaa ggctggcgtt ggtgatatgc
     3061 gcaagctgac aatctcccac cagataacgg agatcgggaa tgattaaacc tttacgcgta
     3121 atgcgtgggc tttcatctaa tgcaatacgt gtcccgagcg gtagccagat gcccgccagc
     3181 gtgggaaccc acagcccgag cgtcatcagc agcgtcaacg gcacaagaat aatcagtaat
     3241 aacagcgcga gaacggcttt atatttaccc agcatgggta gttaatatcc tgatttagcg
     3301 aaaaattaag cattcaatac gggtattgtg gcatgtttaa ccgttcagtt gaaggttgcg
     3361 cctacactaa gcatagttgt tgatgaattt ttcaatatcg ccatagcttt caattaaatt
     3421 tgaaattttg taaaatattt ttagtaactt aaatgtgatt caacatcact ggagaaagtc
     3481 ttatgaaact cgccgtttat agcacaaaac agtacgacaa gaagtacctg caacaggtga
     3541 acgagtcctt tggctttgag ctggaatttt ttgactttct gctgacggaa aaaaccgcta
     3601 aaactgccaa tggctgcgaa gcggtatgta ttttcgtaaa cgatgacggc agccgcccgg
     3661 tgctggaaga gctgaaaaag cacggcgtta aatatatcgc cctgcgctgt gccggtttca
     3721 ataacgtcga ccttgacgcg gcaaaagaac tggggctgaa agtagtccgt gttccagcct
     3781 atgatccaga ggccgttgct gaacacgcca tcggtatgat gatgacgctg aaccgccgta
     3841 ttcaccgcgc gtatcagcgt acccgtgacg ctaacttctc tctggaaggt ctgaccggct
     3901 ttactatgta tggcaaaacg gcaggcgtta tcggtaccgg taaaatcggt gtggcgatgc
     3961 tgcgcattct gaaaggtttt ggtatgcgtc tgctggcgtt cgatccgtat ccaagtgcag
     4021 cggcgctgga actcggtgtg gagtatgtcg atctgccaac cctgttctct gaatcagacg
     4081 ttatctctct gcactgcccg ctgacaccgg aaaactacca tctgttgaac gaagccgcct
     4141 tcgatcagat gaaaaatggc gtgatgatcg tcaataccag tcgcggtgca ttgattgatt
     4201 ctcaggcagc aattgaagcg ctgaaaaatc agaaaattgg ttcgttgggt atggacgtgt
     4261 atgagaacga acgcgatctg ttctttgaag ataaatccaa cgacgtgatc caggatgacg
     4321 tattccgtcg cctgtctgcc tgccacaacg tgctgtttac cgggcaccag gcattcctga
     4381 cagcagaagc tctgaccagt atttctcaga ctacgctgca aaacttaagc aatctggaaa
     4441 aaggtgaaac ctgcccgaac gaactggttt aatcttgccg ctcccctgca atccagggga
     4501 gctgattcag ataatcccca atgacctttc attctctatt cttaaaatag tcctgagtca
     4561 gaaactgtaa ttgagaacca caatgaagaa agtagccgcg tttgttgcgc taagcctgct
     4621 gatggcggga tgtgtaagta atgacaaaat tgctgttacg ccagaacagc tacagcatca
     4681 tcgctttgtg ctggaaagcg taaacggtaa gcccgtgacc tgcgataaaa atccgccaga
     4741 aatcagcttt ggtgaaaaaa tgatgatttc cggcagcatg tgtaaccgct ttagcggtga
     4801 aggcaaactg tctaatggtg aactgacagc caaagggctg gcaatgaccc gtatgatgtg
     4861 cgctaacccg cagcttaatg aactcgataa caccattagc gaaatgctga aagaaggtgc
     4921 acaagtggat ctgaccgcga accagttaac gctggcgacc gcgaaacaga cattaactta
     4981 taagctggcg gatttaatga attaatagct gccacagctc ccggcggcaa gtgactgttc
     5041 gctacagcgt ttgccgttgg gtaatgcaca catcccaatc gccgtaccat ccagttgacg
     5101 ggcaacagaa agcgaaccgc cgatcattgc acaatttgct tctccactac tggacatcga
     5161 cgcttttaaa cctggcgcta cgtgcgccgc agtcgcctgc tgaacaggct cactactaca
     5221 cgccgacaac aataaagcgg cacaccctac ccaaaacgct gctcgcatct cttcttcctc
     5281 tgatcttcaa gccaaacgac accgccataa ataataggca gcacagaggg cgacgtcgag
     5341 agctgtcctg cgcgttgccc cgcattttta cttttttatg gctatttttt tgccctctgt
     5401 ttgatcaaaa cattcattac gctgatgtgg gggacacaaa agcgaaaatg cagaagaaag
     5461 ccatttgcta aaattgaaag attactactg ggcgcgcagc aatttcgtgc gcccctcatt
     5521 tgcgcaatgt aagggtgtca tatgattact attgacggta atggcgcggt tgcttcggtg
     5581 gcgtttcgca ccagtgaagt tatcgccatc taccctatta cccccagttc cacgatggca
     5641 gaacaggctg atgcctgggc cggaaacggc ttgaagaacg tttggggaga cacaccacgc
     5701 gtggttgaaa tgcagtcgga agcgggtgct atcgctaccg tgcatggcgc tttgcagacg
     5761 ggtgcccttt caacatcgtt tacgtcatcg cagggtttgc tgctgatgat cccgacgctg
     5821 tacaaactgg caggcgaact cacaccgttt gtcctgcatg tagcggcacg taccgttgcc
     5881 acacatgcac tctctatttt tggcgatcat tccgacgtca tggcggtgcg ccagacgggt
     5941 tgcgcgatgt tgtgtgcggc aaacgtccag gaagcgcaag actttgctct catttcgcac
     6001 atcgcgacgc tgaaaagccg cgtgccattt attcatttct ttgatggttt ccgtacgtcc
     6061 cacgaaatca ataaaattgt cccgctggcc gatgacacta ttcttgatct catgccgcag
     6121 gccgaaattg atgctcatcg cgcccgggcg ctcaacccgg aacatcccgt gatccgcggt
     6181 acgtcagcca atcctgacac ttatttccag tctcgcgaag ccaccaaccc atggtacaac
     6241 gcggtctatg accatgttga acaggcgatg aatgatttct ctgccgcgac aggtcgtcag
     6301 tatcagccgt ttgaatatta cgggcatccg caagcggaac gggtgattat cctgatgggc
     6361 tctgccattg gcacctgtga agaagtggtt gatgaattgc taacccgtgg cgaaaaagtt
     6421 ggcgtgctga aagttcgcct gtaccgtccc ttctccgcca aacacttgct gcaagctctg
     6481 ccgggatccg tacgcagcgt ggcggtactg gacagaacca aagaacccgg tgcccaggca
     6541 gaaccgctct atctggatgt aatgaccgca ctggcagaag cctttaataa tggcgagcgc
     6601 gaaactctgc cccgtgtcat tggtgggcgc tatggtcttt catccaaaga atttggcccg
     6661 gactgtgtac tgacggtatt tgccgagctc aacgcggcta aaccgaaagc gcgctttacg
     6721 gttggtattt acgatgatgt gaccaatctg tcactgccgt tgccggaaaa caccctgcca
     6781 aactcggcga aactggaagc cttgttttat ggccttggta gtgatggcag cgtttccgcg
     6841 accaaaaaca atatcaagat tatcggtaat tccacgccgt ggtacgcaca gggctatttt
     6901 gtttacgact ccaaaaaggc gggcggcctg acggtttctc accttcgcgt gagcgaacag
     6961 ccgattcgtt ccgcttatct catttcccag gctgattttg ttggctgcca ccagttgcag
     7021 tttatcgata aatatcagat ggctgagcgt ttaaaacctg gcggcatttt cctgctcaac
     7081 acgccgtaca gcgcagatga agtgtggtcg cgcttgccgc aggaagttca ggccgtgcta
     7141 aatcagaaaa aagcgcgctt ctatgtgatt aacgcggcga aaatcgcccg cgaatgtggc
     7201 ctggcggccc gtattaatac cgtcatgcag atggcttttt tccatctgac acaaattctg
     7261 cctggcgata gcgccctcgc agaattgcag ggtgcgattg ccaaaagtta cagtagcaaa
     7321 ggtcaggatc tggtggaacg caactggcag gctctggcgc tggcgcgtga atccgtagaa
     7381 gaagttccgt tgcaaccggt aaatccgcac agcgccaatc gaccgccagt ggtttccgat
     7441 gccgcccctg ctttcgtgaa aaccgtaacc gctgcgatgc tcgccgggct tggtgatgcc
     7501 ctccccgttt cggcgctgcc gccagacggc acctggccga tgggcaccac tcgctgggaa
     7561 aaacgcaata tcgccgaaga gatccccatc tggaaagagg aactctgtac ccaatgtaac
     7621 cactgcgtcg ccgcttgccc acactcagct attcgcgcaa aagtggtacc gcctgaagcg
     7681 atggaaaacg cccctgccag cctgcattcg ctggatgtga aatcgcgtga tatgcgcggg
     7741 cagaaatatg tcttgcaggt ggctccggaa gattgcaccg gttgtaacct gtgcgtcgaa
     7801 gtttgcccgg cgaaagaccg tcagaatcca gagattaaag ccatcaatat gatgtcgcgc
     7861 ctggaacatg tcgaagaaga gaaaatcaat tacgatttct tcctcaacct gccagaaatc
     7921 gaccgtagca aactggaacg tattgatatt cgcacatcgc agctgattac accgctgttt
     7981 gaatattccg gtgcttgctc cggttgtggc gagacgccgt atattaaatt gctgactcag
     8041 ctctatggcg accggatgtt gatcgctaac gccaccggct gttcttcaat ttatggcggt
     8101 aacctgccct ctacaccgta taccaccgat gccaacggtc gtgggccggc atgggcgaac
     8161 tctctatttg aagataatgc cgaatttggc cttggtttcc gcctgacggt cgatcaacac
     8221 cgtgtccgcg tgctgcgtct gctggatcaa tttgccgata aaatcccggc ggaattactg
     8281 acggcgttga aatcagacgc cacgccagag gttcgtcgtg aacaggttgc agctttacgc
     8341 cagcaactca acgatgttgc cgaagcacat gaactgctgc gtgacgcaga tgcactggtg
     8401 gaaaaatcaa tctggctgat tggtggcgac ggctgggctt acgatatcgg ctttggcggt
     8461 ctggatcatg tattgagttt gacggaaaac gtcaacattc tggtgctgga tacgcaatgc
     8521 tattccaaca ccggtggtca ggcgtcgaaa gcgacaccgc tgggtgcagt aactaaattt
     8581 ggcgagcacg gcaaacgtaa agcgcgtaaa gatcttggcg tcagtatgat gatgtacggt
     8641 catgtttatg tggctcagat ttctctcggc gcgcagctga accagacggt gaaagcgatt
     8701 caggaagcgg aagcgtatcc gggaccatcg ctgatcattg cttatagccc gtgtgaagag
     8761 catggttacg atctggcact cagccatgac cagatgcgcc aactcacagc taccggcttc
     8821 tggccgctat atcgcttcga tccgcgtcgt gccgatgaag gcaaactgcc gctggcgctg
     8881 gattcacgcc cgccgtcaga agcactggaa gaaacgttac ttcacgagca acgtttccgt
     8941 cgtctgaatt cgcagcagcc agaagtggca gaacagttat ggaaagatgc tgcagctgat
     9001 ttgcaaaaac gctatgactt cctggcacaa atggccggaa aagcggaaaa aagcaacacc
     9061 gattaaatgc tctggataag gattatccaa ttctaaaaaa aagcccggac gactgttcgg
     9121 gcttgtcttt ttatatgttg gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa
     9181 tctgtgacaa taaaggcata
//
LOCUS       DAZFBR010000158         9161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFBR010000158 DAZFBR010000000
VERSION     DAZFBR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.158"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..234
                     /locus_tag="ACHJZ2_004397"
     CDS             <1..234
                     /locus_tag="ACHJZ2_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4570382.1"
                     /translation="GKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLD
                     HLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            206..2135
                     /locus_tag="ACHJZ2_004398"
                     /pseudo
     CDS             206..2135
                     /locus_tag="ACHJZ2_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2119..2325
                     /locus_tag="ACHJZ2_004399"
     CDS             2119..2325
                     /locus_tag="ACHJZ2_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO4570383.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2322..3914
                     /locus_tag="ACHJZ2_004400"
     CDS             2322..3914
                     /locus_tag="ACHJZ2_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4570384.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEIQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            3904..5409
                     /locus_tag="ACHJZ2_004401"
     CDS             3904..5409
                     /locus_tag="ACHJZ2_004401"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4570385.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5446..5793
                     /locus_tag="ACHJZ2_004402"
     CDS             5446..5793
                     /locus_tag="ACHJZ2_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4570386.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            5851..6102
                     /locus_tag="ACHJZ2_004403"
                     /pseudo
     CDS             5851..6102
                     /locus_tag="ACHJZ2_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            6099..6839
                     /locus_tag="ACHJZ2_004404"
     CDS             6099..6839
                     /locus_tag="ACHJZ2_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4570387.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVR
                     AYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVSAI
                     KVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATG
                     KVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            6853..7284
                     /locus_tag="ACHJZ2_004405"
     CDS             6853..7284
                     /locus_tag="ACHJZ2_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4570388.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            7311..7724
                     /locus_tag="ACHJZ2_004406"
     CDS             7311..7724
                     /locus_tag="ACHJZ2_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4570389.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            7705..>9161
                     /locus_tag="ACHJZ2_004407"
     CDS             7705..>9161
                     /locus_tag="ACHJZ2_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4570390.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALET"
BASE COUNT         2095 a         2243 c         2896 g         1927 t
ORIGIN      
        1 ggaaaactga ttgacaccgg gttctgtatt tttgccctca gtaagctggc aatggcgtta
       61 tccagtacgc ttgattccat ccctttatcc atgcagcgac agtttcctga tttaacaccg
      121 cgccatcttg accatctgaa aacccttatt gctaaggggg caaatcagtg tgcgcgggca
      181 ggggataaat taccggattt acttgatgaa tatatcagag caacaactga ataatatgat
      241 gagcgctgtc acaactgcat tacagcccct gataagggca ttgccggtga cgccagttga
      301 atgggctgat caaaattatt atctgcctaa agaatcttca tatggtgagg gcgaatggaa
      361 aacgctgcca ttccagatcg ccatcatgaa cagtatgggg aatgatcaga tccgcactgt
      421 taatctgatt aaatctgccc gtgttggcta tacaaagatg ttgctggggg gggggcgggt
      481 attttattga gcataaatcc cgaaacagtc tgctttttca gcccacggat tctgccgctg
      541 aagattttat gaagtctcac gtggaggcga cgattcggaa cgtgccatgc ctgaaagacc
      601 tttccccatg gctgggtcgt aaacatcgtg acaatactct cacgctgaaa cgcttttcat
      661 cgggcgtcgg tttctggtgc ctgggcggcg ctgccgccaa aaactaccgt gaaaaatccg
      721 ttgacgtggt ctgctatgac gaactttcct cgttcgagcc ggatgtcgaa aaagagggct
      781 cgccaaccct gctgggggat aagcgtattg aggggtcggt gtggccaaaa tccattcgcg
      841 gctcgacgcc taaaatcaaa ggcacctgcc agatcgaaaa agccgctaac gagtcggcgc
      901 attttatgcg tttttatgtg ccctgcccgc actgtgggga ggcgcagtat ctgaaatttg
      961 gcgatgagtc cacgcctttt gggcttaaat gggagaagga cagccctgaa agtgttttct
     1021 acctctgtga acatcatggc tgcgtgatcc atcagtctga actggaccag agcaacgggc
     1081 ggtggatctg tgaaaacacg ggcatgtgga cccgtgacgg tctgacgttt ttcagcgccc
     1141 ggggtgatga aattccgccg ccgcgctcca tcatgttcca tatctggacg gcgtacagtc
     1201 cgttcaccac ctgggtacag attgtctatg actggctgga tgcactgaaa gatcccaacg
     1261 gcctgaaaac ctttgtgaac accacgctgg gcgagacctg ggaagaggcc gtgggcgaaa
     1321 aactcgatca ccaggtactg atggataagg tggtgcgtta cacggcggcg gtgcctgccc
     1381 gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag atgtatgtct
     1441 ggggatgggc tccgggagag gaagcctttc tggtggataa aatcatcatt atggggcgtc
     1501 ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caacaaaaaa taccgccatg
     1561 cggatggcac cgaaatgact atttcccgtg tctgctggga caccgggggg atcgatggtg
     1621 aaattgttta tcagagatca aaaaaacacg gtgttttccg ggtgctgccg gtaaaaggcg
     1681 catctgtcta tggcaagccg gtgatcacca tgccaaaaac ccgcaatcag cggggcgtgt
     1741 atctgtgtga agtgggaacg gacaccgcaa aagaaattct ctatgcccgt atgaaagccg
     1801 atccctcgcc tgcggatgaa gccacgtcgt atgccatccg ttttcctgat gatccggaga
     1861 ttttttcgca gacagaggcg cagcaactgg tggcggaaga gctggtggag aagtgggaaa
     1921 aaggaaagat gcgtctgctg tgggataaca aaaagcggcg taacgaagcg ctggactgcc
     1981 tggtgtatgc ctacgcggca ttacgtgtgt ccgtgcaacg ctggcagctt gatctggctg
     2041 tactggcaaa atcccgggaa gaagagacga cccggccaac cctgaaagaa ctggcagcga
     2101 agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg tcaggcgcgc
     2161 cttgatttat taaccggtaa acgggtggtg tctgtccaga aagatggtcg cagaattgaa
     2221 tatacggcgg cttctctgga tgagcttaac cgggcgatca atgatgcgga gtcggtactg
     2281 gggacaaccc ggcgtcgccg tcgtccgctg ggagtgaggt tatgaaacga acgcctgtcc
     2341 tgattgatgt gaacggcgtt ccgcttcgtg agagtctcag ctacaacggg ggcggtgcag
     2401 gatttggcgg gcaaatggcg gagtggttgc caccggcgca gagtgccgat gcggccctgc
     2461 tgcccgcgtt gcgtctgggg aatgcccggg cagatgatct ggtgcgcaat aacggaatag
     2521 cggccaatgc ggtggcactg cataaggatc acattgtcgg gcatatgttt cttatcagct
     2581 accgtccgaa ctggcgctgg ctggggatgc gggagaccgc agcaaaaagc tttgtcgatg
     2641 aggtggaggc ggcctggtcg gaatacgccg aagggatgtc tggcgagatc gacgtggaag
     2701 gaaaacgcac gttcacggaa tttatccgtg aaggtgtggg cgttcatgcg tttaacggcg
     2761 aaatctttgt gcagccggtc tgggatacgg aaaccacgca gttattccgt acgcgtttta
     2821 aagccgtgag tccgaaacgg gtggacacgc caggacacgg tatggggaac cgttttctgc
     2881 gggccggggt ggaggtcgat cgatatggcc gtgccgttgc gtaccatatc tgtgaggatg
     2941 attttcctcg ctccgggagt ggacgatggg aacggatccc gcgtgaactt cccaccgggc
     3001 gtccggccat gctgcatatt ttcgagccgg tggaggacgg gcagacccgt ggggccaacc
     3061 agttttacag cgtcatggaa cggctgaaga tgctcgattc cctgcaggca acacagcttc
     3121 agtcggccat tgtgaaagcc atgtatgcag cgacgattga aagtgacctt gataccgaaa
     3181 aggcctttga atatatcgcc ggtgcgccgc aggggcagaa ggataatccg cttattaata
     3241 ttctggagaa gttctccagc tggtatgaca cgaataacgt gacgctgggt ggtgtcaaaa
     3301 ttccgcacct tttccccggg gatgatctga aactacagac tgcgcaggat tcagacaatg
     3361 gattttcggc gcttgaacag gcgctgctgc ggtatatcgc cgccggtctt ggcgtttcct
     3421 acgaacagtt gtcccgtgat tactcgaagg tcagttattc aagtgccagg gcctctgcca
     3481 atgagtcgtg gcgctatttt atggggcggc gaaaatttat tgcggcccgg ctggccacgc
     3541 agatgttttc ctactggctg gaagaggcac ttcttcgggg gattatccgt ccgccacggg
     3601 cgcgttttga tttttatcag gcgcgatcag cctggtcacg ggcagagtgg attggtgccg
     3661 gaagaatggc cattgacggg ctcaaggaga ttcaggaatc ggtgatgcgc attgaggccg
     3721 gactgagcac gtatgagaaa gagccggcgc tgatgggcga ggattatcag gacattttcc
     3781 gccagcaggt cagggaatct gcagagcggc aaaaagccgg actctcacgt ccggtgtgga
     3841 tagcgcaggc gtatcagcag cagatagcgg agagtcgcag gccggaagag gagacaacac
     3901 cccgtgagac gtaatctttc acacattatt gccgcagcat tcaatgaacc gctgcttctg
     3961 gagcccgcct atgcgcgggt tttcttttgc gcgctcgggc gcgagatggg ggcagcaagt
     4021 ctttcggtac cacagcagca ggtacagctt gatgctcccg gaatgctggc tgaaacggac
     4081 gagtacatgg ccggaggtaa acgaccggcc cgtgtttacc gggtggtgaa cggtattgct
     4141 gtactgccgg tgaccggcac gctggtgcac cggctggggg gtatgcggcc attttccgga
     4201 atgacaggct atgacggcat tgtcgcctgt cttcagcagg caatggcgga tagccaggtg
     4261 cggggcgtac tgctggacat tgacagtccg ggcgggcagg ccgccggcgc gtttgactgc
     4321 gctgacatga tttaccgcct ccgtcagcag aagccggtct gggcactgtg caatgacacg
     4381 gcctgttctg cagccatgct gctggcgtcg gcctgctccc gacggctggt tacccagaca
     4441 tcccgtatcg gctccattgg cgtgatgatg agccatgtca gctatgccgg tcatctggcg
     4501 caggccggtg tggatatcac gctgatttac tcaggggcgc acaaggtgga tggcaatcag
     4561 tttgaagcct taccggcaga ggttcgccag aacatgcagc agcgcattga tgcggcgcgc
     4621 cggatgtttg ccgaaaaagt ggccatgttt accggtctgt ctgttgatgc cgtcacggga
     4681 acagaggccg ccgtttttga aggtcagtcc ggcattgatg ccgggctggc ggatgaatta
     4741 gtcaatgcgt cggatgccat cagtgtgatg gccacggcgc tgaacagtaa tgtcagagga
     4801 ggcactatgc cgcaattaac tgcaacggaa gccgccgcgc aggagaacca gcgagtgatg
     4861 gggatcctga catgccagga agcgaaagga cgtgaacagc ttgccacgat gctggcagga
     4921 caacagggca tgagcgttga acaggcccgg gcgattctgg ccgcggcggc accgcagcag
     4981 ccggtggcat ccacgcagag tgaagccgat cgcattatgg cgtgtgaaga agcgaacggt
     5041 cgtgaacaac tggcggcaac gctggcggcg atgccggaga tgacggtgga aaaagcccgc
     5101 ccgatcctgg ctgcttcacc gcaggcggat gccggaccct cactccgtga tcagatcatg
     5161 gcactggatg aggcaaaagg ggctgaggcg caggctgaac agctggctgc ctgcccggga
     5221 atgactgtgg agagcgcccg ggctgtgctg gctgcgggat caggtaaggc agaaccggtc
     5281 tctgcatcca caaccgccct gtttgaacgc atcatggcga accattcacc ggctgcggta
     5341 cagggtggcg tgccacagac gtcagcagac ggtgatgcgg acgtgaaaat gctcatggcc
     5401 atgccatgaa gtcagtgctg accatcaaca ggaggttttt acaatatggt aacgaaaaac
     5461 atcactgaac agcgtgcgga agtacgtatt tttgccggta atgatccggc tcataccgcc
     5521 acaggcagca gcgggatttc ctcggcaaca ccggcactga cgcccctgat gctggatgaa
     5581 gccagcggga aactggtggt ctgggacgga cagaaagccg gtagtgcagt tggcatactg
     5641 gtactgccgc ttgaaggcac agagacggta ctgacctatt acaagtcggg gacctttgcg
     5701 acggaggcaa tccgctggcc tgaaagtgtg gatgaacaca aaaaggcaaa tgcctttgcc
     5761 ggcagtgccc tgagtcacgc ggcgctgccg taacacgtta tcaggccacc gcggtggcct
     5821 gactgatttc tgaatgaaag gaactgattt atgggattgt ttacgacccg ccagttactc
     5881 ggttataccg aacaaaaagt gaaatttcgt gcgctgtttc tggagctgtt tttccgccgt
     5941 acggtgaatt tccataccga agaggtgatg ctggacaaaa ttaccggaaa aacgccggtg
     6001 gcggcctatg tctccccggt tgttgaagga aaagtgctgc gtcatcgcgg tggtgaaacc
     6061 cgcgtgttac gtccgggcta cgtcaagccg aaacacgaat tcccctggag ccggtaaaag
     6121 gagccggtac caccctgtgg gtttataacg gtcagggtga cgcctatgca aacccgttgt
     6181 cagacgatga ctggcagcga ctggctaagg tgaaggatct gacgccgggc gagatgacgg
     6241 cagaatccta cgatgataac tacctggatg atgaagacgc ggactggacc gcgaccgggc
     6301 aggggcagaa atctgcaggt gataccagtt ttacgctggc ctggaaaccg ggagaggaag
     6361 gccagaaagg gcttataggc tggtttgaaa gcggcgatgt ccgggcctat aaaatccgtt
     6421 ttccgaatgg cacggtggat gtgtttcgtg gctgggtcag cagtatcggt aaggccgtga
     6481 cggcgaaaga agtgatcacc cgcacggtga aagtcactaa cgtgggtaaa ccttctgtag
     6541 cggaagaacg cagcaaaatt acgccggtca gtgcgattaa ggtgacgccg acatccggta
     6601 cggtggcaaa agggaaaaca accaccctga cggtttcttt tgagccggaa agtgcaacag
     6661 acaagacgtt cagagcggtt tccgccgatc cgtcgaaagc caccattagt gtgaaagata
     6721 tgacaattac ggtaaacggc gtggcgacag gtaaggtgca gatccctgtg gtgagcggaa
     6781 atggtcagtt cgccgcagtg gctgaagtca ccgttactga agcgggcgct gcagggtaaa
     6841 cggaggtcat acatgtttct gaaaacagaa caatttgaat ataacggtgt gtctgtcacg
     6901 ctttccgaat tgtctgcgct gcagcgtatc gagcatcttg ccctcctgaa acggcgtgca
     6961 gaacaggcag aatccagcgg caacctgcag gtaagcgtgg aagatctcgt cagaaccggc
     7021 gcgtttctgg tggcgatgtc cctgtggcat aaccatccac agaaaacgca gtcaccgtca
     7081 atgaatgagg ccgtgatgaa gatagagcag gaagtgctca ccacctggcc tgccgatgcc
     7141 attgcccggg cggaagacgt ggtgttgtgc ctgtccggga tgagcggggc tgttcgtccg
     7201 gatactgata ttactgaagt ggcgaaaaat aacacgctga ctgatgatga tttttctgcg
     7261 ggaaagtctt cgacggcgag ctgaactttg ccctcagact ggcgcgtgag atggggagac
     7321 ccgactggcg cgccatgctt gccgggatga catccaccga atatgccgac tggcaccgtt
     7381 tttaccgcac gcattatttt caggataccc agctggatat gcatttttcc gggctgacgt
     7441 acgctgtact cagcctgttt ttttgcgatc cggatatgca tccctctgat ttcagtctgc
     7501 ttgtcccccg gcatgaggaa gagcaggtgg agaggccgga tgaggacaaa atgctgatgc
     7561 agaaagcggc aggacttgcc ggaggcgtcc ggttcggtgg ggacggaggg cgcgatattt
     7621 tatcgtctgc ggatgtggcg gatgtcatgg tggatgatgc cgcattaatg atggcttcag
     7681 cggggattcc gggaggtgtg agatatgtcc cagccggttg gtgatcttgt tattgacctg
     7741 agtctggatg ctgtccgttt cgatgagcag atgagccggg taaggcgtca tttttcaggt
     7801 ctggataccg acgtcagaaa aaccgccagt gctgttgaac agggcctgag ccgccaggcg
     7861 ctggctgcac aaaaagccgg gatttccgtc gggcagtata aagcggccat gcgaaccctg
     7921 cccgcacagt ttacggatat cgccacgcag cttgccggtg gtcagaatcc ctggctgatc
     7981 ctgctgcaac agggcggtca ggtgaaggac tccttcggcg ggatgatccc catgttcagg
     8041 gggcttgccg gtgcgatcac cctgccgatg gtcggggtca cctcgctggc ggtggcgaca
     8101 ggtgcgctgg tgtacgcctg gtaccaggga gattccacgc tttcagcgtt taataaaacc
     8161 ctggttcttt ccggtaatca gtccggactg actgccgatc gcatgctgac gctctcaaga
     8221 gccgggcagg cagcagggct gacgtttaac caggcgagag agtcactggc agccctggtg
     8281 aatgccggtg tgcgtggtgg tgaacagttt gatgccatca accagagtgt cgcgcgtttt
     8341 gcttctgcat ccggtgtgga ggtggacaag gttgcagagg ctttcggaaa actgaccacc
     8401 gaccctacgt cggggctgat tgcgatggtg cgccagttcc gtaacgtgac ggcagagcag
     8461 attgcgtatg ttgcgcagct gcagcgttcc ggtgatgagg ccggggcctt acaggcggcg
     8521 aacgatatcg ccacgaaagg ctttgatgag cagacccgtc gcctgaaaga aaacatgggg
     8581 acactggaga cctgggcgga taaaaccggg aaggcattca aatcgatgtg ggatgccatt
     8641 ctggatatcg gtcgtcctga gtcctcagcg gatatgctcg ccagtgcaca gaaggcattt
     8701 gatgaggcgg ataaaaaatg gcagtggtac cagagccgga gccagcgccg gggaaaaacc
     8761 gcctctttcc gggccaacct tcagggcgca tggaatgacc gggaaaatgc ccgtctgggg
     8821 ctggcagcgg ccacgctgca gtcggatatg gaaaaagccg gtgaactggc cgccagggac
     8881 cgggccgaac gggacgcatc acagctgaag tataccggag aggcgcagaa ggcgtatgag
     8941 cgtctgctga cgccgctgga gaaatatacc gcccgtcagg aagaactgaa taaggccctg
     9001 aaagacggga aaatcctgcg ggcggattac aacacgctga tggcggcggc gaaaaaggat
     9061 tatgaatcga cgctgaaaaa gccgaagtcg tcaggagtca aagtgtcagc cggtgagcgt
     9121 caggaagacc aggcgcatgc tgccctgctg gcgcttgaaa c
//
LOCUS       DAZFBR010000159         8976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFBR010000159 DAZFBR010000000
VERSION     DAZFBR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.159"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1348
                     /gene="paoC"
                     /locus_tag="ACHJZ2_004408"
     CDS             <1..1348
                     /gene="paoC"
                     /locus_tag="ACHJZ2_004408"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO4570391.1"
                     /translation="TLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYA
                     GANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQ
                     IDPADPTRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLL
                     EKSGARVHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPV
                     SAGSGGQWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAILHEA
                     TAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILN
                     PKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLD
                     DTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            1358..2314
                     /gene="paoD"
                     /locus_tag="ACHJZ2_004409"
     CDS             1358..2314
                     /gene="paoD"
                     /locus_tag="ACHJZ2_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO4570392.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(2493..3620)
                     /locus_tag="ACHJZ2_004410"
     CDS             complement(2493..3620)
                     /locus_tag="ACHJZ2_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4570393.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWMVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(3762..4820)
                     /locus_tag="ACHJZ2_004411"
     CDS             complement(3762..4820)
                     /locus_tag="ACHJZ2_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4570394.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            5066..5968
                     /locus_tag="ACHJZ2_004412"
     CDS             5066..5968
                     /locus_tag="ACHJZ2_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4570395.1"
                     /translation="MKGNVSMAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSR
                     SVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGT
                     VRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQ
                     GMVAVRITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDD
                     KKITHQPEGQWAFSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRME
                     GLFLYYPHRNVSPALRMVIDTLKI"
     gene            6205..6267
                     /locus_tag="ACHJZ2_004413"
     CDS             6205..6267
                     /locus_tag="ACHJZ2_004413"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570396.1"
                     /translation="MNTVVFVATVEYAYLSLHFY"
     gene            complement(6671..6949)
                     /locus_tag="ACHJZ2_004414"
     CDS             complement(6671..6949)
                     /locus_tag="ACHJZ2_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570397.1"
                     /translation="MSLYIKLILSIVREISVNTICSLIVVVALSLLSFSSVAKTITAV
                     GSTINSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(7116..7838)
                     /locus_tag="ACHJZ2_004415"
     CDS             complement(7116..7838)
                     /locus_tag="ACHJZ2_004415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4570398.1"
                     /translation="MDKVILITGASSGIGEGIARELGMTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
     gene            7937..8836
                     /locus_tag="ACHJZ2_004416"
     CDS             7937..8836
                     /locus_tag="ACHJZ2_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4570399.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
BASE COUNT         2208 a         2361 c         2314 g         2093 t
ORIGIN      
        1 cacccttcag cacttgcgta tcggtgccga ccagagcggg aaaatcaccg ctatctcaca
       61 tgaaagctgg tctggaaacc tgcccggcgg cacgccggaa acggcggtac agcaaagcga
      121 attactctac gccggggcaa accgtcatac cggcctgcgg ctcgccacgc ttgatttgcc
      181 ggaagggaac gccatgcgtg cgcccggcga agcccccggt ctgatggcgc tcgaaatcgc
      241 gatcgacgaa ctggcggaaa aagcgggcat cgatcccgtc gagtttcgca tcctgaatga
      301 cactcagatt gaccccgccg acccgacgcg ccgcttctct cgccgtcagc ttatcgagtg
      361 cttgcgcacc ggagcggata aatttggttg gaagcagcgc aacgccacac ccggacaggt
      421 gcgcgacggg gagtggctag tcggccacgg cgtcgcggcg ggctttcgca ataatctgct
      481 ggaaaaatcg ggggctcggg ttcacctcga accaaacggc accgttaccg tggaaacgga
      541 catgaccgac attggcaccg gcagctacac cattctggcc cagacggcag cggaaatgct
      601 tggcgtaccg ctggagcagg ttgcggttca cctcggcgat tccagtttcc cggtttctgc
      661 gggttctggt ggacaatggg gcgcgaatac ctccacctcc ggcgtttacg ccgcctgtgt
      721 gaagcttcgc gaaatgattg cctcggcagt cgggtttgat cctgagcagt cgcagtttgc
      781 cgacggcaag attaccaacg gtacccgaag cgccatacta catgaggcca ccgcaggcgg
      841 cagactgaca gcggaagaga gcattgaatt cggaacactg agcaaggagt accagcagtc
      901 gacctttgcc gggcattttg tggaggtcgg cgtgcatagc gcgacgggag aagttcgggt
      961 ccggcgtatg ctcgctgtgt gtgctgcagg acgcatcctg aatccgaaaa ctgcacgcag
     1021 ccaagtcatt ggcgcaatga ctatgggcat gggcgcggca ctgatggagg agctggcggt
     1081 ggatgaccgt ttgggctact tcgttaatca cgatatggcg gggtatgagg tgccggtcca
     1141 tgcggatatc ccaaaacagg aagtgatttt cctggatgat accgacccca tatcctcccc
     1201 gatgaaggcc aaaggtgtcg gtgagctggg cctgtgcggc gtgagcgcgg ctatcgccaa
     1261 cgcggtgtat aacgccaccg gtattcgggt acgcgattat cccatcactc tggataagct
     1321 gctcgataag ctgccggatg tggtttaagg aggaacaatg tcatacccgc tttttgataa
     1381 agacgaacac tggcataagc cagagcaggc gtttctcacc gatgaccacc ggaccattct
     1441 gcgcttcgcc gtagaggcgc taatgtccgg taaaggagcg gtgctggtga cgctggtgga
     1501 gatacgcggc ggcgcggcgc gcccgctcgg ggcgcagatg gtggtgcgcg aagatggtcg
     1561 ttactgcggt tttgtctctg gcggctgcgt ggaggccgct gccgcttttg aagcgctgga
     1621 gatgatgggc tcaggctgcg atcgcgaaat tcgctatggc gaaggttcgc cgtggtttga
     1681 catcgttctg ccctgcggcg gtgggatcac gctgacgctc cataaactac gctcggcaca
     1741 gcctctgctc gccgtgctga accgcctgga acagagaaaa ccggcggggc tgcgctacga
     1801 tccgcaagca caatcgttgg tgtgcctgcc cacgcaaacc cgaacgggct ggaatctcaa
     1861 tggctttgag gtggggttca ggccatgcgt caggctgatg atttacggac gttctcttga
     1921 ggcgcaggca accgcgagtc ttgcagcagc cacaggctat gacagccata tcttcgatct
     1981 ttttccggcc tcagccagcg ctcagatcga taccgatacg gcggtcattt tgctgtgcca
     2041 tgatctcaac cgggagctgc cagtgttgca ggccgcgcga gaagcaaaac ccttttatct
     2101 cggcgcattg ggcagctatc gaacccacac gttacgtctg caaaagctcc acgagctggg
     2161 atggtccagg gaggagacag cgcaaatccg ggcacccgtc gggatattcc ccaaagcccg
     2221 gaatgcgcat acactggccc tctccgtgct ggcagaagtc gcctctgtac gtctccatca
     2281 ggaggaggat tcatgcctgc ccccgtcgtc ctgatccttg cggccgggcg tggaaagcgc
     2341 tttctcacct ccgggggaaa taccataaat taagagaaaa atttgcagta gccgttttag
     2401 aaaatcacct aaatcgttta aaagagcgcc ctacccatag tggttgctca tcgccttgtg
     2461 taactttttc agcaacgcgc gccctcgccg gactaaagcg caccggcctt ctggcgtgct
     2521 gtaacaaagg tcagtacgcc cgcgcaaagg agcagcacac tactcatggc aaacgtactc
     2581 tgccagccaa ggcggtcaaa cacgatgcct cctgccgttg atccaagggc gattgagagc
     2641 tgaatcaccg cgaccatgag cccgcctccg gcttcggcgt tgtcaggcaa cgtacgcgca
     2701 atccatgtcc accatccggt tggcgcggcg gtggccagca tgccccagag tcctaacagc
     2761 agcgagacca tccagacgtg atgcccggta agcatcagac cgatggcaat gacggccatc
     2821 agtccgggta tggccatcag cgtggggtag aattttctgt tgagaaatat cgaaacaacc
     2881 agcgtgccaa taaaaccggc cacgccgata atcagcaaaa tcaacgacaa gcctaaggaa
     2941 ttaacccgtg tcaccgactc cagaaatggg cgcacatagg tgaatagcgc aaactgcccc
     3001 atgaaaaaca ggccacaagc tatcatgccg accacaacca tgcggcggct aaataagcgg
     3061 aataccgcgc cacatgacgt gtggctttta ttcgcatcca tgctgggcag actaatgcac
     3121 tgccagataa aggcgacaac cgccatgggc accaagcaca agaaagcgcc tcgccatccg
     3181 accgtagccc ccagatagct tcccagcggc gcggccacga ccgtcgccag cgcattaccg
     3241 gcgttgaaaa tcgccagcgc acgcgtgacc tgatgctgcg gcaccagacg aatcgccgtt
     3301 gccgctgaca tcgaccagaa tccgccaatg gccacaccaa tcatcgcacg accgaccata
     3361 tacatcagat aactggtagc cagcgcaata ataagccccg atacggccat cagaaccgtc
     3421 atccccagca gcaggaactt acgattcatc tttccagcta gcgttgaaag cgtcaggctg
     3481 gtcagaaccg ccagcgcacc ggagatagca atcccccgcc ctgccagccc ctccgtcacg
     3541 cccaggtcac gggcaatcgg cgtgagcagg ctgacgggca taaattcaga agcaatcagc
     3601 acaaacacgc acagggtcat ggcaaaaata ccgccccagt atgcgtgctg atgatgaggt
     3661 gatggtgttt ggcttagtgt cgacatagtg aaatttcaca aagatcagga taaaagccac
     3721 cggccataga ccggtggctt tgcatcaatt caggggaggt tttatttcag tttggtctgg
     3781 aaaaattgtt cgaacttcgc gaaaggtatt tttccggcca cgttatcgta caggtccacg
     3841 tgatttgccc ctggtaccac caccaactct ttgtctttac tgccaaccgc cttgtaagca
     3901 tcttcagcaa aatagcgtga atgtgctttc tcaccggtca caatcagcgt cggaatggtg
     3961 atttcactgg cgtagctcaa cagcggcata ttcataaatg acagcggcat cgttgccgtc
     4021 cacgccccgg tagagttaac ggaacgttcg tggaagccgc gcggcatgcg atagtagtca
     4081 aagaactctt tcagtaccgg atgaggattt gccggcagcg tttctggcag aatgcgctcg
     4141 cccgcactga ccttgccgtt gctatcgaca ttaatatcat gaccaccgtg agcgaacgtt
     4201 ccgctctgcg catccttcca gcgctgttca ttcaaatatt gcagaacagc acgacggtcg
     4261 gcggtagaat aacggtcttc gccatccccc acaccatggc ccatcgcccg gctcatgtca
     4321 tacatcacgc tggtagcaac cgctttaacg cgggtatcca tcgccgcgtc gttcagcgcc
     4381 atgccgcccc agccacaaat accgagcagc ccgatacggt tgcgatctac ctctttttgc
     4441 agccctaaga aatccaccgc cgcgctgaaa tcttcagtgt tgatatccgg tgaggcgaca
     4501 tttcgcggat agccgccgct ttccccggtg taagaaggat cgaacgccag ggtaacaaac
     4561 cctcgttccg ccagcgtctg cgcatacaag ccgctggatt gctctttcac cgcgccaaat
     4621 ggcccactga ccgcaatcgc cgccagcttg cgatcgccac ggtctttagg caggtaaaga
     4681 tcgcccacta aggtgatgcc atatcggttc gggaacgaga ctttacggtg atcgactttc
     4741 tggctctcgg cgaacgtttt atcccattta tcggtcatgg acacaggggc attaggattg
     4801 gttgaatcag cataactcat tgtcgtgact ccacttaatg atgcgaaaag cagcatgacc
     4861 ggcatcgcgg tttttagcgt tcgggtgaag gctttcataa aaatccccat aaaaaacaac
     4921 agagagatac aggtttccga cggacaacgc tgcggtgctt tactttgatg gggtcattat
     4981 agtgagcaga attagtgctg attagggggc gattaatgct aagattaata ccatattgtt
     5041 ataaattaat gctgagattg ccggcatgaa ggggaatgtt tcaatggcaa aaagggagaa
     5101 ctacaacgag ctatacctgt ttatgcaggt ggtgcgggaa ggcagcttta ccgcagcggc
     5161 taagcggctg ggtcttgctc agtcaggagt cagccgttct gttcgcgagc ttgaagaaag
     5221 gctgggcgtc cagctgctgg tgcgcaccac gcgtaaactg tcgctgacgc aagcaggcga
     5281 gcagctctac cagaagacgg catctggatt tgaaatgtta gatcttgggc ttgccacgct
     5341 ggcgcactat cgagagacgc cctccggcac ggtgcgtatc aatgccagcc agcacgcgat
     5401 tgataaatgc ctgctgccaa agctggcggt atttaaacag cgatatcctg atatccagct
     5461 tgaactgata aatgagagcc ggttcgtcga tatcatcgag gaaagattcg atgcaggtgt
     5521 ccgcctgggg cctgaagtca gccagggcat ggtcgcggta cgcatcacgc ccgatatgga
     5581 gatggcaatt gtggggactc cggaacattt tcgtcgctat ggttttccgc aaaccccggc
     5641 ggatttaaag gcgcatccct gtatcgccta tcagtttgcc gacggcagcg tataccagtg
     5701 ggaactcaat caggatgata aaaaaatcac tcatcaaccc gaaggacaat gggccttttc
     5761 cgacagctat atggaagcag aagccgcccg gctgggcctc gggttggcct atgttcctgt
     5821 tgaactggtc gcggatgatt tagaacacgg taagcttatc agagtgttgc agcgttacag
     5881 cctgcgaatg gagggattgt ttctctatta ccctcatcgc aacgtctccc ccgctctgcg
     5941 gatggtcatt gatacattga aaatctgaac gttaacaacg accgactctt cactgtgcaa
     6001 tattgtgtca ggtagtcact ctgcgttcaa ttccaggggc cgctttgagc gagaaagagc
     6061 ggacttatct gtacaagggg actatccagc aatggagatc atccaaaaga aaaaaccacc
     6121 tggctcccct cgacaacctt aatccgggca tttagcctca atcaacattt tcatatctgt
     6181 caggttactt cattatgaaa accgatcaat accgttgttt ttgttgcaac agtagaatac
     6241 gcgtatttat ccctccattt ctattaataa cggcaataaa ctgttcattt cactgatatt
     6301 taaaatatgc atccttttca agctgttcag cggcgtaaga aaacgtatta aatatgaatt
     6361 cgacgattta accatttctg tctgattggt aatcatctgt gattaatgct ggtctattga
     6421 ttactccagc attaaccgct tagggatgta ttcttcctgt tgatatacag ccctcgaaaa
     6481 agagagaaaa taaaactgtg tccaatatta ctctaccact ccagaccgaa gcatcggtag
     6541 aattagcaga acgactggta caatagattg ggatagcgtc atttgtcagg tattccattg
     6601 cctgacaaat gacaattttg agtcaataga cgtgcttctt gatcatgtct taaaaattat
     6661 accatgagcc ttatgaagca tcatttttac tgactggtgt taactttgct atcatataaa
     6721 ctctgttctt aaaaaacgcg cccagaattt tatatgattt cccttgtttt tctgcctgta
     6781 aagaaatttc tttttcagtg ctgttaatgg ttgaaccgac ggcagtaatc gttttggcga
     6841 cgctactgaa tgataataaa gacagtgcaa ccacaacaat taaagaacag atagtattca
     6901 cgctaatttc cctgacaatt gagaggatga gttttatata taaagacatt aaacatgagt
     6961 catgtttact tcctgtgaaa aataatcttt ctgtaattaa accttcatct ccttcggggt
     7021 gagcaataca taatgtatct tgctatgaaa aacacaggga tatataaata ttccttctga
     7081 cctatttaat aaaacgtgaa tgtaaaagtt attcttcagt ttgcagatgc agtgggtctg
     7141 atggtaattt cggttgtatc aacgctttcg ggagattcaa taatatgcct tacagcacgg
     7201 gcaatatccg cgggttgaag tgcaacagaa cggtaggcgt ccattgcctt catggtttcc
     7261 acatgggtaa tggttgaagc cagttcactt tcgacaacgc caggatttac gcaggtcaca
     7321 cggattttcg agctttcctg ccgtaagcca tcagaaatag cccgtactgc aaattttgat
     7381 gcacaataga ctgcgcctgt gggcacaaca gaaagggcac caatagaacc aagattgatt
     7441 atctgaccgg aaccctgtgc ttccatcacc ggaagtacag cgccaattcc ccacagtaca
     7501 cctttgatat tcacgtcaat agtgagcgcc cattcatctt gttttcctgc tgcaagcggt
     7561 gaaagcggca taacgcccgc attattgata agaacatcaa ctcgccccca gctatccagc
     7621 gctgcttgca cgaaatcggc catggactgt cggtctgtga catccaactc ccgcgcttta
     7681 gcaattcctc ctgcgcggca gatttcggtt gcaatggctt cgatacgctc aactctgcgt
     7741 gctcccagta aaacctttgc acctgtcatt ccaagctctc tggcaatacc ttccccaata
     7801 ccacttgatg cgccggtaat taatatgacc ttatccatta cgttctcctt caaatgtgtt
     7861 cggagagaga atagaacttt atgattggtt ccagtatcct gctttcaatg tactcagtgg
     7921 ttagcggcat taaacaatga aaatcgatct taatctttta cccattttta tcgcggtagc
     7981 agaagagagt aattttagta aagccgccgc acggctgggc gttacccgtt cagcggtcag
     8041 tcagggaata agacgccttg aagatgcatt cgggacaatg cttgtaatgc ggaccacgcg
     8101 ctcagttaat ttgacagaag cgggagaacg gctgcataaa tcgctgtcat tacctttatc
     8161 cggtattgag gcagcgttcg aagagatggt ttctgaccat gtgccacgcg gacttctcag
     8221 aatcgcggtg acgtcaatag ctgaagaatt tctttcaggt ccgctaatcg cttcgtttgc
     8281 agcagccaat ccagccgtta cgcttgatat tgttgtcacg gatgaggaat tcgatatcgt
     8341 ggccgcaggt tacgatgctg gcgtaaggct cggagaggtc attgaaaaag acatgattgc
     8401 tgtgcccctt accggcaggc agcgtgaaat ggtcgttgcc tctccctcct atctcgcagc
     8461 caacagcaca cctgttcatc cgcgtgaact tgtgaatcat aagtgtatcg gctggcgtca
     8521 gtccccggag gttgcgcctt atcgctggcc atttgaagaa aacgggagag ctttcgacct
     8581 tgcgattgaa ccgcagatta cgactaatga tctgcgtctg atgttgaggc tggctcttgc
     8641 cggcggagga ataacaattg ccactcagga aactttcagg ccgtatattg aaagcggtaa
     8701 gcttgtatct ctgcttgatg actttcttcc acaatttccg ggcttctatc tgtatttccc
     8761 acagcgtcgc aatattgcac caaagctccg cgctctgatt gaccacgtta aggaatggcg
     8821 gcagcaattg gcttaaatgt ctgcacctgc attgcctgat gtcagaacag tattttgatt
     8881 aattgccagg gttacaatgg cacaaatgcg gcacaggagg aaacgtggtg tatttaaata
     8941 tggggtaact acttgattta aatggtgcga taatag
//
LOCUS       DAZFBR010000160         8718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFBR010000160 DAZFBR010000000
VERSION     DAZFBR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.160"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(344..607)
                     /gene="rpsT"
                     /locus_tag="ACHJZ2_004417"
     CDS             complement(344..607)
                     /gene="rpsT"
                     /locus_tag="ACHJZ2_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO4570400.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            710..934
                     /locus_tag="ACHJZ2_004418"
     CDS             710..934
                     /locus_tag="ACHJZ2_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO4570401.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            942..1883
                     /gene="ribF"
                     /locus_tag="ACHJZ2_004419"
     CDS             942..1883
                     /gene="ribF"
                     /locus_tag="ACHJZ2_004419"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO4570402.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1926..4742
                     /gene="ileS"
                     /locus_tag="ACHJZ2_004420"
     CDS             1926..4742
                     /gene="ileS"
                     /locus_tag="ACHJZ2_004420"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO4570403.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4742..5236
                     /gene="lspA"
                     /locus_tag="ACHJZ2_004421"
     CDS             4742..5236
                     /gene="lspA"
                     /locus_tag="ACHJZ2_004421"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO4570404.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5324..5773
                     /gene="fkpB"
                     /locus_tag="ACHJZ2_004422"
     CDS             5324..5773
                     /gene="fkpB"
                     /locus_tag="ACHJZ2_004422"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4570405.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5775..6725
                     /gene="ispH"
                     /locus_tag="ACHJZ2_004423"
                     /gene_synonym="lytB"
     CDS             5775..6725
                     /gene="ispH"
                     /locus_tag="ACHJZ2_004423"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO4570406.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6791..7705
                     /gene="rihC"
                     /locus_tag="ACHJZ2_004424"
     CDS             6791..7705
                     /gene="rihC"
                     /locus_tag="ACHJZ2_004424"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO4570407.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7872..8693
                     /gene="dapB"
                     /locus_tag="ACHJZ2_004425"
     CDS             7872..8693
                     /gene="dapB"
                     /locus_tag="ACHJZ2_004425"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO4570408.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
BASE COUNT         2002 a         2171 c         2464 g         2081 t
ORIGIN      
        1 gttaataatt tacaaggtct aatgaggaga tatttatgaa tggttaaagt atataggaat
       61 ttaattcaaa aatagagtgg tcttaagttt aatacctcaa tgtgtatcac aatatccaca
      121 ttctttgtag gggagtctgg agattgagta gatattcttg ttcagaatgt atcagccgat
      181 ggttctacga ttcttaagcc acgaagagtt cagatagtgc aacggcatgt ctcttttgac
      241 tatctggcaa ccggcagtgt gttctctcac gcatcacaaa agcagcaggc ataaaaaaac
      301 ccgcttgcgc gggctttttc acaaagcttc agcaaattgg caattaagcc agtttgttga
      361 tctgtgcagt caggttagcc ttatgacgtg cagctttgtt tttgtggatc agacctttag
      421 cagcctgacg gtccacgatc ggttgcattt cgttaaatgc tttctgtgca gcagctttgt
      481 cgccagcttc gatagctgcg tatactttct tgatgaaagt acgcatcata gagcgacggc
      541 ttgcgttgtg cttacgagcc ttttcagact gaatggcgcg cttcttagct gatttgatat
      601 tagccaaggt ccaactccca aatgtgttct atatggacaa ttcaaaggcc gaggaatatg
      661 cccttttagc cttcttttgt caatggattt gtgcaaataa gcgccgttaa tgtgccggca
      721 ctcgttacgt agtgatggcg caggattcta ccagcttgcg gggtgtgaat acagcttttc
      781 cgcgataaaa attgcagcag gcagtcagtt tcttcccgtg atttgcgcca tgaccatgac
      841 aatgaaaagc cacttctttc tgatttcggt actcaatcgc cggttaacct tgaccgctgt
      901 acaaggtata ctcggacgat tttcactgtt ttgagccaga catgaagctg atacgcggca
      961 tacataatct cagccaggcc ccgcaagaag ggtgtgtgct gactattggt aatttcgacg
     1021 gcgtgcatcg cggtcatcgc gcgctgttac agggcttgca ggaagaaggg cgcaagcgca
     1081 acttaccggt gatggtgatg ctttttgaac ctcaaccact ggaactgttt gctaccgata
     1141 aagccccggc acggctgacc cgactgcggg aaaaactacg ttaccttgca gagtgtggcg
     1201 ttgattacgt gctgtgcgta cgtttcgaca ggcgtttcgc ggcgttaacc gcgcaaaatt
     1261 tcatcagcga tcttctggtg aagcatttgc gggtaaaatt cctcgccgtg ggggatgatt
     1321 tccgctttgg cgctggtcgg gaaggcgatt tcttgttatt acagaaagct ggcatggaat
     1381 acggcttcga tatcaccagt acgcaaactt tttgcgaagg tggcgtgcgt atcagcagca
     1441 ccgccgtgcg tcaggccctt gcggatgaca atttggctct ggcagaaagt ttactggggc
     1501 acccgtttgc catctccggg cgtgtagtcc acggtgatga attagggcgc actataggtt
     1561 tcccgacggc gaatgtaccg ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg
     1621 tagaagtgct gggcctcggt gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc
     1681 caacggttgc cggtattcgc cagcagctgg aagtgcattt gttagatgtt gcaatggacc
     1741 tttacggtcg ccatatacaa gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg
     1801 cttcgctgga cgaactgaaa gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt
     1861 ttgggctaac aaaaccggct taagcctgtt atgtaatcaa accgaaacac ggaaccgaga
     1921 atctgatgag tgactataaa tcaaccctga atttgccgga aacagggttc ccgatgcgtg
     1981 gcgatctcgc caagcgcgaa ccgggaatgc tggcgcgttg gactgatgat gatctgtacg
     2041 gcatcattcg tgcggctaaa aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt
     2101 atgcgaatgg cagcattcat attggtcact cggttaacaa gattctgaaa gacattatcg
     2161 tgaagtccaa agggctttcc ggttatgact cgccgtatgt gcctggctgg gactgccacg
     2221 gtctgccgat cgagctgaaa gtagagcaag aatacggtaa gccggatgag aaattcaccg
     2281 ccgccgagtt ccgcgccaag tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca
     2341 aagactttat ccgtctgggc gtgctgggcg actggtcgca cccgtacctg accatggact
     2401 tcaaaactga agccaatatc atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc
     2461 acaaaggcgc gaagccggtg cactggtgcg tagactgccg ttctgcactg gcagaagcgg
     2521 aagttgagta ttacgacaaa acttctccgt ccattgacgt cgctttccag gcggtcgatc
     2581 aggatgcgct gaaagcgaaa tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga
     2641 tctggaccac tacgccgtgg actctgcctg cgaaccgcgc aatctctatt gcacctgatt
     2701 tcgactatgc gctggtgcag atcgacggtc aggccgtgat tctggcgaaa gatctggttg
     2761 aaagcgtaat gcagcgtatc ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg
     2821 cggagcttga gctgctgcgc tttgcccatc cgtttatggg cttcgacgtc ccggcaatcc
     2881 tcggcgatca cgttaccctg gatgccggta ccggtgccgt tcacaccgcg cctggccacg
     2941 gcccggacga ctatgtgatc ggtcagaaat acggcctgga aaccgctaac ccggttggcc
     3001 cggacggcac ttatctgccg ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag
     3061 cgaacgacat cgtcgttgcg ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa
     3121 tgcagcacag ctatccgtgc tgctggcgtc acaaaacgcc gatcatcttc cgcgcaacgc
     3181 cgcagtggtt cgtcagtatg gatcagaaag gtctgcgcgc gcagtctctg aaagagatca
     3241 aaggtgtgca gtggatcccg gactggggcc aggcgcgtat cgagtcgatg gtcgctaacc
     3301 gtcctgactg gtgtatctcc cgtcagcgta cctggggcgt accgatgtct ctgttcgtgc
     3361 acaaagacac ggaagagctg catccgcgta ccctcgaact gatggaagaa gtggctaaac
     3421 gcgttgaagt tgatggcatc caggcgtggt gggatcttga tgcgaaagag atcctcggcg
     3481 atgaagctga tcagtatgtg aaagtgccgg atacgctgga tgtatggttt gactccggct
     3541 ctactcactc ttctgttgtt gacgtgcgcc cggaatttgc cggtcacgct gcggacatgt
     3601 atctggaagg ttctgaccaa caccgtggct ggttcatgtc ttctctgatg atctccaccg
     3661 cgatgaaggg caaagcgccg tatcgtcagg tgctgaccca cggctttacc gtagatggtc
     3721 agggccgcaa gatgtctaaa tccatcggca acaccgtgtc gccgcaggat gtgatgaaca
     3781 aactgggcgc ggatattctg cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg
     3841 ccgtttctga cgagatcctg aaacgtgctg ccgacagcta tcgtcgtatc cgtaacaccg
     3901 cgcgcttcct gctggcaaac ctgaacggtt ttgatccggc aaaagatatg gtgaaaccgg
     3961 aagagatggt agtactggat cgctgggccg taggttgtgc gaaagcggca caggaagaca
     4021 tcctcaaggc atacgaagca tacgatttcc acgaagtggt acagcgtctg atgcgcttct
     4081 gctccgttga gatgggttcc ttctacctcg acatcatcaa agaccgtcag tacaccgcca
     4141 aagcggacag cgtggcgcgt cgtagctgcc agactgcgct gtatcacatc gcagaagcgc
     4201 tggttcgctg gatggcacca atcctctcct tcaccgctga tgaagtgtgg ggctacctgc
     4261 cgggcgaacg tgaaaaatac gtcttcaccg gcgagtggta cgaaggtctg tttggtctgg
     4321 cagacagtga agcaatgaac gatgcgttct gggacgagct gttgaaagtg cgtggcgaag
     4381 tgaacaaagt cattgagcag gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg
     4441 cagtaacctt gtatacagaa ccggaactgg cggcgaaact gaccgcattg ggcgatgaat
     4501 tacgatttgt cctgttgacc tccggcgcta ccgttgcaga ctataacgac gcacctgctg
     4561 atgctcagca gagcgaagtg ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg
     4621 agaagtgccc acgctgctgg cactacaccc aggatgtcgg caaggtggcg gaacacgcag
     4681 aaatctgcgg ccgctgtgtc agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct
     4741 gatgagtcaa tcgatctgtt caacagggct acgctggctg tggctggtgg tagtcgtgct
     4801 gattatcgat ctgggcagca aatacctgat cctccagaac tttgctctgg gggatacggt
     4861 cccgctgttc ccgtcgctta atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt
     4921 ccttgccgat agcggcggct ggcagcgttg gttctttgcc ggtattgcga ttggtattag
     4981 cgttaccctg gttgtgatga tgtatcgctc gaaggccacg cagaagctaa acaatatcgc
     5041 ctacgcgctg attattggcg gcgcgctggg caacctgttc gaccgcctgt ggcacggctt
     5101 cgttgtcgat atgatcgact tctacgtcgg cgactggcac ttcgccacct tcaacctcgc
     5161 cgatactgcc atctgtgttg gtgcggcact gattgtgctg gaaggttttt tgccttctaa
     5221 agcgaaaaaa caataataaa ccctgccgga taaggcctac agattgctgc gaaatcgtag
     5281 gccgcatccg gcaaaaatcc tcaaaatata agagcaaacc tgcatgtctg aatctgtaca
     5341 gagcaatagc gccgtcctgg tgcacttcac gctaaaactc gacgatggca ccaccgccga
     5401 gtctacccgc aacaacggta aaccggcgct gttccgcctg ggcgatgctt ctctttccga
     5461 agggctggag caacacctgc tggggctgaa agtgggcgat aaaaccactt tctcgctgga
     5521 gccagatgcg gcgtttggcg tgccgtcacc ggacctgatt cagtacttct cccgccgtga
     5581 gtttatggat gcaggcgagc cagaaattgg cgcaataatg ctttttaccg caatggatgg
     5641 cagtgagatg cctggcgtga tccgcgaaat taacggcgac tccattaccg tcgatttcaa
     5701 ccatccgctg gccgggcaga ccgttcattt tgatattgaa gtgctggaaa tcgatccggc
     5761 actggaggcg taacatgcag atcctgttgg ccaacccacg tggtttttgt gccggggtag
     5821 accgcgctat cagcattgtt gaaaacgcgc ttgccattta cggcgcaccg atatatgtcc
     5881 gtcacgaagt ggtgcataac cgctacgtgg tcgatagcct gcgcgagcgt ggagctatct
     5941 ttattgagca gatcagcgaa gtgccggacg gcgcgatcct gatcttctcc gcacatggtg
     6001 tttctcaggc ggtacgtaac gaagcgaaaa gccgtgattt gacggtattc gacgccacct
     6061 gtccgctggt gaccaaagtg catatggaag tcgcccgcgc cagccgtcgt ggcgaagagt
     6121 ctattctcat cggtcacgcc gggcacccgg aagtggaagg gacgatgggg cagtacagca
     6181 accctgaagg gggaatgtat ctggtcgaat cgcctgacga tgtgtggaaa ctgacggtca
     6241 aaaacgaaga gaagctctcc tttatgaccc aaaccacgct gtcggtagat gacacgtctg
     6301 atgtgatcga cgcgctgcgt aaacgcttcc cgaaaattgt cggtccgcgc aaagatgaca
     6361 tctgctacgc cacgactaac cgtcaggaag cggtacgcgc cctggcagaa caggcggaag
     6421 ttgtgttggt ggtcggttcg aaaaactcct ccaactccaa ccgtctggcg gagctggccc
     6481 agcgtatggg caaacgcgcg tttttgattg acgatgcgaa agatatccag gaagagtggg
     6541 tgaaagaggt taaatgcgtc ggcgtgactg cgggcgcatc ggctccggat attctggtgc
     6601 agaatgtggt ggcacgtttg cagcagctgg gtggtggtga agccattccg ctggaaggcc
     6661 gtgaagaaaa tattgttttc gaagtgccga aagagctgcg tgtcgatatt cgtgaagtcg
     6721 attaagtcat tagcagccta agttatgcga aaatgccggt cttgttatcg gcatttttta
     6781 tggagaaaac atgcgcttac ctatcttcct cgatactgac cccggcattg acgatgccgt
     6841 cgccattgcc gccgcgattt ttgcccccga actcgacctg caactgatga ccaccgtcgc
     6901 gggtaatgtc tcggttgaga aaactacccg taacgccttg caactgctgc atttctggaa
     6961 tgcggagatt ccgctcgccc agggagccgc tgtgccgctg gtacgcgcac cgcgtgatgc
     7021 agcatctgtg cacggcgaat cgggaatggc tggctacgac tttgttgagc acaaccgaaa
     7081 gccactcggg ataccggcgt ttctggcgat tcgggatgcc ctgatgcgtg caccagagcc
     7141 cgttaccctg gtggccatcg gcccgttaac caatattgcg ctgttacttt cacaatgccc
     7201 ggaatgtaaa ccgtatattc gccgtctggt gatcatgggt ggttctgccg gacgcggcaa
     7261 ctgtacgcca aacgccgagt ttaatattgc tgccgatcca gaagctgctg cctgtgtctt
     7321 ccgcagtggt attgaaatcg tcatgtgcgg tttggatgtc accaatcagg caatattaac
     7381 tcctgactat ctctctacac tgccgcagtt aaaccgtacc gggaaaatgc ttcacgccct
     7441 gtttagccac taccgtagcg gcagtatgca aagcggcttg cgaatgcacg atctctgcgc
     7501 catcgcctgg ctggtgcgcc cggacctgtt cactctcaaa ccctgttttg tggcagtgga
     7561 aactcaaggc gaatttacct caggcacgac ggtggttgat atcgacggtt gcctgggcaa
     7621 accagccaat gtacaggtgg cattggatct gaatgtaaaa ggcttccaac agtgggtggc
     7681 tgaggtgctg gctctggtgc cgtaacctgt cacatgttat cggcatgcag tcattcatcg
     7741 actcatgcct ttcactgata tccctccctg tttatcatta atttctaatt atcagcgttt
     7801 ttggctggcg gcgtagcgat gcgctggtta ctctgaaaac ggtctatgca aattaacaaa
     7861 agagaatagc tatgcatgat gcaaacatcc gcgttgccat cgcgggagcc ggggggcgta
     7921 tgggccgcca gttgattcag gcggcactgg cattagaagg cgtgcagctg ggcgcggcgc
     7981 tggagcgcga aggatcttct ttactgggca gcgacgccgg tgagctggct ggtgcaggga
     8041 aaacaggcgt taccgtgcaa agcagtcttg atgcgataaa agatgatttt gatgtgttta
     8101 tcgattttac ccgtccggaa ggtacgctga accatctcgc tttttgtcgc cagcatggca
     8161 aagggatggt gatcggcact acggggtttg acgaagccgg aaaacaggca attcgtgacg
     8221 ccgccgccga tattgcgatt gtctttgccg ccaattttag cgttggcgtt aacgtcatgc
     8281 ttaagctgct ggagaaagca gccaaagtga tgggtgacta caccgatatt gaaattattg
     8341 aagcacatca tagacataaa gttgatgcgc cgtcaggcac cgcactggca atgggagagg
     8401 cgatcgccca cgcccttgat aaagatctga aagattgcgc ggtctacagt cgtgaaggcc
     8461 ataccggtga acgtgtgcct ggtaccattg gttttgccac cgtgcgtgca ggtgacatcg
     8521 ttggcgaaca taccgcgatg tttgccgata ttggcgagcg tctggagatc acccataagg
     8581 cgtccagccg catgacattt gctaacggtg cggtaagatc ggctttgtgg ttgagtggta
     8641 aggaaaatgg tctttttgat atgcgagatg tgcttgatct caataatttg taaccacaaa
     8701 atgtttatga tgatgcaa
//
LOCUS       DAZFBR010000161         8622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFBR010000161 DAZFBR010000000
VERSION     DAZFBR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.161"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1892
                     /gene="espL4"
                     /locus_tag="ACHJZ2_004426"
     CDS             <1..1892
                     /gene="espL4"
                     /locus_tag="ACHJZ2_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO4570409.1"
                     /translation="APIFEEYLMEILPQRSDALDKKELNLNSDRKEKEFPRVKLNGQC
                     YFPGRPQNRIVCRHIAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALI
                     SSRPEGRCVAACGDFGLVMKAYFDKMESNDLSVMAAILLVDNHALTVRLRIKNTTEGC
                     IHYVVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAII
                     PALPKEQLLMLKGTVDEITPPLSPATMNLLMAIGQNHQLKQLMIQLQKMPELHRTEML
                     TAYNSINLPGLYLAINYGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSG
                     LFLAISRKDKNVVTSILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDA
                     DMLKIVLDALSLLIRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEAL
                     PSQAQEINISASDIVDLLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFD
                     KKNMKPLLLANNSNEYPGLFSAIQHKQQNVVEMVYLALSDHARLFGFTAEDIMDFWQH
                     KAPQKYSAFELACELGHRVIAELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKAS
                     PHTVR"
     gene            complement(1861..3597)
                     /gene="aceK"
                     /locus_tag="ACHJZ2_004427"
     CDS             complement(1861..3597)
                     /gene="aceK"
                     /locus_tag="ACHJZ2_004427"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO4570410.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3781..5085)
                     /gene="aceA"
                     /locus_tag="ACHJZ2_004428"
     CDS             complement(3781..5085)
                     /gene="aceA"
                     /locus_tag="ACHJZ2_004428"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO4570411.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5115..6716)
                     /gene="aceB"
                     /locus_tag="ACHJZ2_004429"
     CDS             complement(5115..6716)
                     /gene="aceB"
                     /locus_tag="ACHJZ2_004429"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO4570412.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6985..7914)
                     /gene="metA"
                     /locus_tag="ACHJZ2_004430"
     CDS             complement(6985..7914)
                     /gene="metA"
                     /locus_tag="ACHJZ2_004430"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO4570413.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8071..8514
                     /locus_tag="ACHJZ2_004431"
     CDS             8071..8514
                     /locus_tag="ACHJZ2_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO4570414.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
BASE COUNT         2215 a         2105 c         2112 g         2190 t
ORIGIN      
        1 ttgctccaat atttgaagaa tacctaatgg aaattttacc tcaacgctca gacgctcttg
       61 ataaaaaaga attaaatcta aactcagata gaaaagaaaa agaattccca agagttaagc
      121 ttaatggtca atgttatttt ccggggcgcc cccaaaaccg tatagtatgc cgacacattg
      181 ctgcacaata tattaatgat atttatcaga atgttgacta caaaccccat caagatgatt
      241 actcttcagc tgaaaaattt ctcactcact tcaacaaaaa atgcaaaaac cagactttgg
      301 cgttgatttc cagccgtcct gaggggcgtt gcgttgctgc ctgcggtgat ttcgggctag
      361 ttatgaaggc atattttgac aagatggaat caaatgacct cagtgttatg gctgccatat
      421 tattggtaga taaccatgct ttgacggtcc ggctaagaat aaagaacaca actgaaggat
      481 gtatccatta cgtggtttcg gtttatgatc ctaatgtaac taacgataaa ataagaatca
      541 tgagcgaaag taaagaggat attaaacact attcactgat ggattttatg aatgtagatt
      601 atagcctcct gaaatggtca aatgatcatg ttattaacca atctgttgca ataattccag
      661 cacttccaaa agaacagcta ttgatgctaa aaggaactgt ggatgaaata acccctccat
      721 tatcacctgc aacgatgaat ttgctaatgg caattggtca gaatcaccaa cttaagcaac
      781 tgatgattca actccagaaa atgccagaac tacatagaac agaaatgttg actgcctata
      841 atagtattaa cctccccggt ttatatttgg ctataaatta tggtaatgcg gatatcgttg
      901 agactatttt taattcattg tcagagccag gatatgaagg attactctcg aaaaaaaatc
      961 tcatgcatat tctggaggca aaagataaaa atggtttttc tggattattt ttagcgatat
     1021 cacgtaagga taaaaatgtt gtaacctcga ttctgaacgc cttacctaaa ctggccgcaa
     1081 cacatcattt agataacgaa caagtgtata aattcctgag tgccaaaaat agtacgtcca
     1141 gccatgtttt ataccatgtt atggcgaatg gtgatgccga catgctgaaa attgttttgg
     1201 acgcgttatc tttgttaatt cgcacatgtc atttgactaa agaacaggta ctcgatctcc
     1261 tgaaggcaaa ggatttttat ggttgcccag gactatacct ggcgatgcaa aatggacata
     1321 gcgatatcgt gaaagttatt cttgaagcat tgcccagcca ggcccaggaa attaacattt
     1381 cagcttccga tattgtagat cttctgaccg ctaaaagtct tgcgcgcgac acgggtttgt
     1441 ttatggccat gcagcgcgga catatgaacg ttattaatac tatttttaac gcattaccca
     1501 ctctgtttaa tacgtttaaa ttcgataaaa aaaatatgaa gcccctcctc ctggcaaata
     1561 attctaatga atacccaggt ttgttttcag cgatacagca taaacagcaa aacgttgtag
     1621 agatggttta tcttgcttta tctgaccatg cacgcctgtt tggatttacc gctgaagata
     1681 ttatggattt ttggcaacac aaagcgccac aaaaatactc tgcctttgag ttggcttgtg
     1741 aattgggtca ccgggttatt gctgaattaa tctttaatac attaaataag atggctgaaa
     1801 gctttggctt tacggataac cctcgataca ttgcggagaa aaattatatg gaagctttac
     1861 tcaaaaaagc atctccccat accgtacgct aaatctttgc ctgcgccgat acgcataaac
     1921 atcttccaca tgcccttcac gtatgcggtt ttgtagtgcg cgccagtaat cagcgcggaa
     1981 caggtcggcg tgcatctctt caaacagcgg accaatacgc gggtcggcgc atagccaatg
     2041 gcgaaactct tccgggaaaa catcgcccgg cgagacgctg taccacggtt cgctggcaag
     2101 ttcgtcttcc gggtagcgcg gcggcgggat atcgcggaaa ttcacttccg tcatgtagca
     2161 aatttcatcg taatcataaa aaaccacacg cccgtgacgg gtgacaccga agtttttaaa
     2221 cagcatgtcg cccgggaaaa tgttagcagc ggcaagctgg cgaatggcat tgccgtactc
     2281 ttcaatggcg tcgcgcagct gctgaccttc cacttgttcc agccagatat tgagcggcac
     2341 catccgccgc tcaatataaa gatggcgaat cacaatttgt tcgccgagat cggtgatttt
     2401 ttccgctgct tcctggagca gtaattccat taatgccggg gaaatatgcc gcttctccag
     2461 cacaaagttt tcaaactcct gggtatccgc cattcggccc acgcgatcgt gctctttcac
     2521 cagttgatag caggcacgaa cgtgagcggc agacatctct ttctgcggcg cgaacttgtc
     2581 tttgatgact ttaaatacac ggtcaaagcc cggcagcgtg aacaccaaca tcaccatgcc
     2641 acgaataccc ggcgcttcaa tgaactgctc attacagccc tgtagataaa cgagatattc
     2701 gcggtagctt tcggttttgg cgtgcttctg gcagccgata gccatataca attcagcggt
     2761 ggttttacct ggcagaattt cccgtaacca ctcgaccagc gctgcgggca gcggcgcata
     2821 aaccataaaa taagaacgcg caaagccaaa aacaatgctc gcttcggcgg tcgtcgtcag
     2881 acaggtatca ataaacagtt cgccgtcatc cgtctggtgg atcggcaata aaaatggcaa
     2941 tgtgccggaa ggcgttatta gtttgcccac cagccaggcg gctttattac ggtaaaacag
     3001 ttcgttcgcc acctgtaaat gacttttgga gaggttttct ggccccaacg tttccgtcag
     3061 atggcgaata atgtaatgga tgtcacggct tttattctgc cagtgcagac gcaacggcag
     3121 gtcgctgata acgcgcatca gcagagattc ccagccgtga tcggggtgaa agtctttcgc
     3181 cagcgggcgg ggaatggtac gaaagcggcg ctctggctgg gagctaaaga taaaaagccg
     3241 ctcgggagta agcgagcggt ggtcaaataa ccgacagtac acggagttaa aaaagctctc
     3301 cgcaatctcg aagcgcgggt aatccggcaa cagccgggtg taatgctctt tgacgcgcag
     3361 taaaaattcc gcgtccgtgc tttggccgtt ggtaatgcag cgcagttgct ccacgaccag
     3421 accaacgtga tgatcgtaaa gatggatacg gtttttcatc gcctgctgga cagcatgcca
     3481 gtcggcttgt tcgaaacgct gctgcgcccc ggaagtcact tcgaggaatc gaccatactg
     3541 agcgtcgaag ccttgcaaaa tggtttgagc aatcagtaat tccaggccac gcggcattta
     3601 gcgccctcat caggagcaga gaattgccgg atgcggcgta aacgccttat ccagcctacg
     3661 ttcggcaacg gttgtaggca tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg
     3721 attgccggat gcggcgtata tgccttatcc ggcctacagt cagcaacgtt tgtaggttgc
     3781 ttagaactgc gattcttcgg tggagccagt cagcgcggta accgacgacg tgccgccctg
     3841 aataatggtc gtcactttat cgaagtaacc tgtacccact tcctgctggt gagatacgaa
     3901 ggtgtagcca tctttcgcgg cggcgaattc cggctgctgc actttctcaa catagtgctt
     3961 cataccctcg ccctgggcat aggcgtttgc cagatcaaac atgttgaacc acatgctgtg
     4021 gatacctgcc agggtgatga actggaactt gtagcccata tccgacagct gctgctggaa
     4081 gctggcaata gtgttgtcgt cgaggttttt ctgccagttg aacgacggcg agcagttata
     4141 agccagcagt ttgcccggat atttcgcgtg gatagcttcc gcaaagcgac gcgccagttc
     4201 cagatccggc gtggaggttt cacaccatac caggtcagca tatggcgcat acgccaggcc
     4261 acggctgatc gcttgctcaa tgcccgcatg agtacggaag aagccttcac tggtacgctc
     4321 gccggtaata aattcgctgt catacgggtc gcaatcggag gtgatcagat ccgccgcatc
     4381 agcatcggta cgtgcaacca gcagggttgg aacgcccgtc acgtcagctg ccagacgcgc
     4441 cgcgaccagt ttctgaatag cttcctgagt tggcaccaaa actttgccgc ccatgtgacc
     4501 gcatttcttc actgacgcca gctgatcttc gaagtgaact gccgctgcac cggcttcaat
     4561 catcgctttc atcagttcaa aggcattcag gacaccgcca aaaccggctt ccgcgtcggc
     4621 aacgatcggc aggaagtaat cgacatagcg cggatcgccc ggctcaatgc ccgcggacca
     4681 ttggatctga tccgcacggc ggaaagtgtt gttgatccgc tccaccacag ctggcaccga
     4741 gtttgccgga tagagcgact gatccggata catgctggcc gccaggttag cgtccgccgc
     4801 tacctgccat cccgacagat agaccgcttc aatacccgct ttcgcctgtt gcagcgcctg
     4861 accgccagtc agtgcaccga ggctgttgat gtagcctttt ttcgactcgc catgcagcag
     4921 acgccacatt ttcgccgcgc ccagttgcgc cagcgtgcat tcaggattga ctgaaccgcg
     4981 taatttcacc acatcttccg cgctgtatgg gcgagtaatt ccttcccaac gcggttgagt
     5041 ccactctttc tgtaattctt caatttgttg tgtacgggtt ttcatgtgca gatgctccat
     5101 agttatgtgg tggtttacgc taacaggcgg tagcctggca gggtcaggaa atcaattaac
     5161 tcatcggaag tggtgatctg ttccatcaag cgtgcggcat cgtcaaaacg cccctgggag
     5221 aaacgttctt cgcccagttc gctggcaatg actttcatct cttcgcccag catctggcgg
     5281 aacaaggctt tagttaccgg tttgccattg ctcaacgttt tttgatgatg gatccactgc
     5341 cagattgagg tacgggaaat ttcagccgtc gccgcatctt ccatcaggcc ataaatcggc
     5401 acgcagccgt tgccggagat ccatgcttcg atgtactgca cagccacgcg aatgttggcg
     5461 cgcatacctt cttcggtgcg ttcaccatca caaggtgcca gcagctgatc ggcagtaatc
     5521 ggcgcgtctt gttcgcgcat cacttcaagc tgatttttac gggagccgag aatgtcgttg
     5581 aataccgcca ttgccgtgtc cgcaaggcct gggtgagcga tccatgtgcc atcgtgaccg
     5641 ttattggctt ccagcgattt atccgctttt actttgttga gcacctggtt attgtgctct
     5701 tcatctttgc tcggaataaa cgccgccatg ccgcccatcg caaaagcacc gcgtttatgg
     5761 caggttttaa tcaacaggcg tgagtaagca ttcaggaatg gtttatccat cgttactgcc
     5821 tgtctgtctg gcaggacgcg atcgggatag tttttcaacg ttttgatata gctgaagatg
     5881 taatcccaac gaccgcagtt cagaccaaca atatggtcac gcagcgcgtg aaggatttca
     5941 tccatctgga acacggcggg cagcgtttca atcagcaacg tcgccttgat ggtgccgcgc
     6001 ggcagattaa agcgatcttc tgcatagctg aagacttcgc tccaccaggc cgcttcctgc
     6061 caggactggg ttttcggcag atagaaatag ggaccactgc cctttgccaa cagtgcctga
     6121 tagttgtgga agaaatagag cgcaaaatca aacaggctgc cggggattgc ctcaccacgc
     6181 caggtgacat gtttttccgg caagtgcaga ccgcgtaccc gacaaatcaa aaccgctgga
     6241 ttgggcttga gctggtaaat tttgcctgct tcattggtgt aactgatggt gccgttaacc
     6301 gcatcacgca ggttaatttg cccgtcgatc actttgttcc agtctggtgc cagtgaatct
     6361 tcgaaatcgg ccataaagac tttcacattg gcgttgagcg cgttgatcac catcttgcgc
     6421 tctacggatc cggtaatctc tacgcggcga tcttctaagt ccgcaggaat cccgcgaatt
     6481 ttccagtcag catcacgaat ggaagctgtt tccgaaataa aatcaggcaa cgttccgtta
     6541 tcaatatctt gctgctgctg aatgcgcgct gccagaagtt tattgcgttg tggcgtaaaa
     6601 tgcgtcacca gctcagtcag aaattctacc gcttcggcag taagaatttg cttctcctgc
     6661 tcgccatacg gccttatgaa agccagttca tcggttgttg ttgcctgttc agtcatcgtg
     6721 cagctcctcg tcatggatcc gaaaacttcc ccactgtgaa cgaaggatcg ttgtgcgctt
     6781 ttcgttcagc acaactaaga ctactcattt aaaaatcaaa atcaaaaaca atttccattt
     6841 taattaaaaa tacttgataa cttattgata acaatatgaa taaaatttat tcacctactg
     6901 aggtgtgttt cggaaataaa aaaggcaccc gaaggtgcct gaggtaaggt gctgaatcgc
     6961 ttaacgatcg actatcacag aagattaatc cagcgttgga ttcatgtgcc gtagatcgta
     7021 tggcgtgatc tggtagacgt aatagttgag ccagttggta aacagcaaat taccgtgact
     7081 acgccagctc gctcgcggtt tgttttgcgg atcattgtgc gggaaatagt tatacggtac
     7141 ttccgggtct agtccggctt ccacatcgcg gaaatattcc tgcgccagcg tttgcgcatc
     7201 atattcggga tggcccgtca caaaggcaat gcgcttatct ttactggcaa acagatatgc
     7261 atccccttct tccgtctctg ccagaatttc cagatcggtg taatcacgaa tcaacgctgc
     7321 cggaaagtca gcatagcgcg aatgcggtgc caagaatgaa tcatcaaagc cacgcgtcag
     7381 aagcgcatga ggatggagaa tatgatgctc gtaaacgcca gagagtttgt cggtgcgagt
     7441 ttgcttagga atgccgtaga ggatattgag cgctgcctgt accgcccagc agacaaacag
     7501 cgtcgaggtg acgtgatctt tcgaccactc cagcacctgt ttgatctgcg gccagtaagc
     7561 gacatcatta aactccacca ggcccagcgg cgcaccggtt acaatcagac cgtcaaagtt
     7621 ctgttcctga atatcttcaa agttacagta gaagttgttc agatgctctg cgggcgtgtt
     7681 acgcgattca cgggaatcga tgcgcaacag ctgaatatcg acctgcaaag gtgagtttga
     7741 aagcaggcgc agaaactgat tttcagtttc aatcttcttc ggcatcaggt taaggatcag
     7801 aaccttaagt ggacgaattt cctgaccaga cgcacgagaa gttgtcatca caaagacgtt
     7861 ttcttcacgc aagaaattga cggcgggtag ctcgtccggc acacgaatcg gcataacctg
     7921 attacctcac tacatacgct tatacgttta gacatccaga tagctgaaga taaccagaaa
     7981 atttgccaat gtcgagcctg catgttgaag gtgagaaagt ttcaaggaaa agactacgtt
     8041 agaatataag aatgactaaa aggagagaaa atggttatta gtattcgccg ctcacggcat
     8101 gaggaagggg aggaactcgt tgcgatttgg tgtcgatctg tcgatgccac ccacgatttt
     8161 ttatcagcag agtatcgggc cgagctggaa gagctggttc gttcctttct gccggaagca
     8221 ccgttgtggg tcgcggttaa tgaacgggat cagccggttg ggtttatgtt gttaagtggg
     8281 cagcatatgg atgcgctgtt tatcgatcct gatgtgcgcg gctgcggcgt gggccggatg
     8341 ctggtgaagc atgcgctctc aatggccccg gaactgacaa ccaatgttaa tgagcaaaat
     8401 gagcaggcgg ttgggttcta taagaaggtg ggttttaagg ttacgggacg ctctgaggtg
     8461 gacgatttgg ggaaaccgta tccgttgctg gatctggcgt atgtggggga ataagctgcc
     8521 ccctatcgtg cctgcgtaag ctggataaaa cgcgccagcg tcgcatccgg catttttttg
     8581 caccaacgca aaaaggccat ccgtcaggat ggcctttcgc tt
//
LOCUS       DAZFBR010000162         8616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFBR010000162 DAZFBR010000000
VERSION     DAZFBR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.162"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(177..389)
                     /gene="rpmE"
                     /locus_tag="ACHJZ2_004432"
     CDS             complement(177..389)
                     /gene="rpmE"
                     /locus_tag="ACHJZ2_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO4570415.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            592..2790
                     /gene="priA"
                     /locus_tag="ACHJZ2_004433"
     CDS             592..2790
                     /gene="priA"
                     /locus_tag="ACHJZ2_004433"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO4570416.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2946..3971
                     /gene="cytR"
                     /locus_tag="ACHJZ2_004434"
     CDS             2946..3971
                     /gene="cytR"
                     /locus_tag="ACHJZ2_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO4570417.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4063..5022
                     /gene="ftsN"
                     /locus_tag="ACHJZ2_004435"
     CDS             4063..5022
                     /gene="ftsN"
                     /locus_tag="ACHJZ2_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO4570418.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5115..5645
                     /gene="hslV"
                     /locus_tag="ACHJZ2_004436"
     CDS             5115..5645
                     /gene="hslV"
                     /locus_tag="ACHJZ2_004436"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO4570419.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5655..6986
                     /gene="hslU"
                     /locus_tag="ACHJZ2_004437"
     CDS             5655..6986
                     /gene="hslU"
                     /locus_tag="ACHJZ2_004437"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO4570420.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7053..7979
                     /gene="menA"
                     /locus_tag="ACHJZ2_004438"
     CDS             7053..7979
                     /gene="menA"
                     /locus_tag="ACHJZ2_004438"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO4570421.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            8072..8557
                     /gene="rraA"
                     /locus_tag="ACHJZ2_004439"
     CDS             8072..8557
                     /gene="rraA"
                     /locus_tag="ACHJZ2_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO4570422.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         2094 a         2232 c         2352 g         1938 t
ORIGIN      
        1 gccggttttc cgctcatcgt aaaatccttt tgaaataaat aagaaccaga agcctagcgc
       61 cagtcaacaa caggaaatga taatgagtta acaccccgga atttttcaga atttataaaa
      121 ggcaaattac aggcacaaaa aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat
      181 ttgctgcccg ggatgttgaa acgcttgttg aagcggtcaa cacggccacc ggtagcaaca
      241 tcacgctgtt tgccagtgaa gaacgggtgg cacttgctgc acacgtcgag gttcaggtca
      301 tgaccaacgg tggagcggat tttcattacg ttaccgcaag agcagctagc agtaatttct
      361 tcgtatttcg ggtgaatatc ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc
      421 ttccagccct aacgccagac accacgcgat gttaaaagta tagcttcaat acgatcattt
      481 cgtacgaagg cgcgaaatca tacagaaatt aaccagcata tgcaaactga tccgcactct
      541 tctacggcaa tgtgtatact aacccaccga atttcaagtc aggatgatgc tatgcccgtt
      601 gcccacgttg ccttgcccgt tccgcttcct cgtacctttg actatctcct gccagaaggc
      661 atggcggtta aagccgggtg tcgcgtgcgc gtgccgtttg gcaaacagca ggagcgcatc
      721 ggggttgtgg tgtcagttag cgacgtcagt gagctgccgc ttaacgagct aaaagcggta
      781 gtcgaagtgc tggatagtga gccggtgttt actcactccg tctggcgatt gctgctctgg
      841 gcggcggatt actatcatca tccgattggc gatgtgctgt ttcatgcctt gccgatttta
      901 ctgcgccagg ggcggcctgc ggcgaacgcg ccgatgtggt actggtttgc cactgaacaa
      961 ggccatgcgg tggatctgaa cagcctgaaa cgttcgccaa agcaacaaca ggcactggcg
     1021 gcattgcggc aaggcaaaat ctggcgcgac caggttgcca cgcttgaatt taatgatgcc
     1081 gcgttacagg cgctgcgcaa aaaaggtctg tgtgatttag caagtgaaac accagagttt
     1141 agcgactggc gaacgaacta tgccgtttct ggtgaacggt tgcgattgaa taccgaacag
     1201 gccaccgccg ttggcgcaat tcatagcgcg gcggatacct tctctgcttg gctgctggcg
     1261 ggcgttacag gttccggtaa aacagaggtt tatttgagcg tactggaaaa cgtgctcgct
     1321 cagggcaaac aggcgctggt gatggtgccg gaaatcggcc tgacaccgca aactatcgcc
     1381 cgttttcgtg aacgttttaa tgcccccgta gaagttctgc attccggcct gaacgacagc
     1441 gagcgtcttt cggcgtggtt gaaagcgaaa aatggtgagg cggcgattgt catcggcacc
     1501 cgctccgcgc tgtttacgcc gtttaaaaat ctcggtgtga ttgtcattga tgaagagcac
     1561 gacagttcct acaagcagca ggaaggctgg cgctatcatg cccgcgacct ggcggtttat
     1621 cgtgcgcaca gcgagcaaat cccgattatt cttggctccg caacacccgc actggaaacg
     1681 ttatgcaacg tccagcagaa aaaataccgc ctgctgcgcc tgacgcgtcg ggcggggaat
     1741 gcgcgtccgg caattcaaca tgtgctggat ttaaaaggtc agaaggtgca ggcggggctg
     1801 gctccggcgt taatcacccg tatgcgccag catttacagg ccgacaacca ggttatcctc
     1861 tttcttaacc gccgtggctt tgcgcctgcc ttgctgtgcc acgactgcgg ctggattgcc
     1921 gaatgtccac gttgcgatca ctactacacg ctgcatcagg cgcaacagca tttgcgctgt
     1981 caccactgtg acagccaacg cccggtgccg cgccagtgcc cttcctgcgg ttccacgcac
     2041 ctggtacccg tggggctggg caccgaacag cttgaacaga cgctcgcgcc gttattccct
     2101 ggtgtgccca tttctcgtat cgaccgcgat accaccagcc gcaaaggagc gctggaacaa
     2161 caactggcgg aagtgcatcg cggcggcgcg aggatattga ttggcacgca aatgctggcg
     2221 aaaggtcacc actttccgga tgtgacgctg gtagcgttac tggatgtgga cggcgcgctg
     2281 ttttctgccg attttcgctc ggcagagcgt tttgcccagc tatacaccca ggtagcaggc
     2341 cgtgccgggc gtgcgggtaa acagggcgaa gtggtgctgc aaacgcatca tccggaacac
     2401 ccgctattgc aaacgttgct ctataaaggc tacgacgctt ttgccgaaca ggcactggct
     2461 gagcggcgaa tgatgcaact gccgccgtgg accagccatg tgattgtgcg tgcggaagat
     2521 cataacaatc agcacgcgcc attgttcctg caacaactgc gtaatctgat cctctccagc
     2581 ccgctggcag acgacaaatt gtgggttctc ggtccggttc cggcactggc acctaaacgt
     2641 ggcggtcgct ggcgctggca gatattgttg cagcaccctt cccgcgtgcg cttgcaacac
     2701 atcattaacg gtacgctggc actcatcaat acaataccgg attcccgtaa ggtgaaatgg
     2761 gtgctggatg ttgatccgat tgagggttaa accgctcacg atgcgaggcg gatcgaaaaa
     2821 ttcaatattc atcacacttt tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta
     2881 aaaatgttgc cgatgtgaag taaacatgga tgtagtacgc ctgacgtgcc aggcgaggag
     2941 tgagtgtgaa agcgaagaag caggaaactg ccgcgaccat gaaagacgtt gccctcaagg
     3001 caaaagtctc tacagcgacc gtctcccgag cattaatgaa tcccgataaa gtctcccaag
     3061 ccacccgtaa tcgggttgaa aaagcggccc gtgaagtggg ttatttaccg cagcctatgg
     3121 ggcgcaacgt caagcgtaat gaatcccgca ccattctggt gattgtcccg gatatctgcg
     3181 atcccttctt tagcgaaatt attcgcggta tcgaagttac ggcggcaaat cacggatatc
     3241 tggtgctgat tggcgactgt gcgcatcaaa atcagcagga aaaaaccttt atcgatttga
     3301 tcatcaccaa gcaaattgat ggcatgttgc tgctgggttc aaggctgccg tttgatgcca
     3361 gcattgagga acagcgtaat ctgccgccga tggtgatggc gaacgaattt gcaccggaac
     3421 tggagctgcc taccgttcat atcgacaatc tgaccgccgc atttgatgca gtaaattatt
     3481 tatatgagca agggcataaa cggattggct gtatagccgg tcccgaagag atgccgctgt
     3541 gtcactaccg cctgcaaggt tacgttcagg cgctgcgtcg ctgcggcatt atggttgatc
     3601 cgcaatacat cgcccgtggc gacttcacct tcgaagccgg aagcaaagcg atgcagcagc
     3661 tgcttgatct tccacaaccg cctactgctg tcttctgcca tagcgatgtg atggcgctcg
     3721 gcgcactttc tcaggcaaaa cgccaggggc tgaaagtccc ggaagacctt tccataatcg
     3781 gttttgataa catcgacctg acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc
     3841 cgcgttacga aatcggtcgg gaagctatgc tgttattgct tgatcaaatg caggggcaac
     3901 acgttggcag tggctctcgt ttaatggact gcgaacttat catccgggga tcaacacgcg
     3961 cgttacctta aagtaaacct ttaagactgc cgtgactggt caaaggcccg ccgcttaagt
     4021 aacatggcgg gctgacgaac gaataaatac agcgaaacga tagtggcaca acgagattat
     4081 gtacgccgca gccaaccggc accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa
     4141 cgaaatctgc ctgcggtttc tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc
     4201 tttatcggtg gtctgtactt cattacgcat cacaagaaag aagagtccga gacgctgcaa
     4261 agccagaaag tgaccggaaa cggactacca ccaaaaccag aagaacgctg gcgctacatc
     4321 aaagagctgg aaagtcgcca gccgggtgtg cgtgcgccca cagaaccttc tgccggtggt
     4381 gaagtgaaaa cgccggagca actgacacca gaacaacgtc agcttcttga acaaatgcag
     4441 gctgatatgc gccagcagcc aacgcagctg gttgaagttc cgtggaatga acagacgccg
     4501 gaacagcgtc agcaaacgct acagcgccaa cgtcaggcgc agcaattagc ggaacagcaa
     4561 cgcctggctc agcagtcgcg tacgactgaa caaagctggc agcagcagac gcgtacgtcg
     4621 caagccgcgc cggtacaggc gcaaccgcgc cagtctaaac cggcttctac tcagcagccg
     4681 taccaggatc tgctgcaaac ccctgcgcac acgaccgcgc agtcgaagcc gcagcaagct
     4741 gcgccggtta ctcgtgccgc tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc
     4801 cgctggatgg tgcagtgcgg ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct
     4861 cagctggcgt tcgaaggctt tgactcaaaa attaccacca acaatggctg gaatcgtgtg
     4921 gtcattggcc cggtgaaagg caaagagaac gcagacagca ccctcaatcg gttgaagatg
     4981 gcgggtcata caaactgcat tcggctcgcc gccgggggtt gaaaccctca aaatcccccc
     5041 catctataat tgcattatgc cccgtacttt tgtacggggt ttgtactctg tattcgtaac
     5101 caaggggtca gctcgtgaca actatagtaa gcgtacgccg taacggccat gtggtcatcg
     5161 ctggtgatgg tcaggccacg ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc
     5221 gccgtctgta caacgacaaa gtcatcgcgg gctttgcggg cggtactgcg gatgctttta
     5281 cgctgttcga actgtttgaa cgtaaactgg aaatgcatca gggccatctg gtcaaagccg
     5341 ccgttgagct ggcaaaagac tggcgtaccg atcgcatgtt gcgcaaactt gaagcactgc
     5401 tggcagtcgc ggatgaaact gcatcgctta tcatcaccgg taacggtgac gtggtgcagc
     5461 cagaaaacga tcttattgct atcggctccg gcggccctta cgcccaggct gcggcgcgtg
     5521 cactgttaga aaatactgaa cttagtgctc gtgaaattgc tgaaaaggcg ttggatattg
     5581 caggcgacat ttgcatctat accaaccatt tccacaccat cgaagaatta agctacaaag
     5641 cgtaaggatc tcccatgtct gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc
     5701 acatcatcgg ccaggacaac gccaagcgtt ccgtggcgat tgctctgcgt aaccgctggc
     5761 gtcgcatgca gctcaacgaa gagctgcgcc atgaagtgac cccgaaaaat atcctgatga
     5821 tcggcccgac cggtgtcggt aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg
     5881 cgccgttcat caaagttgaa gcgaccaaat tcaccgaagt gggctacgtc ggtaaggaag
     5941 tggattctat tattcgcgat ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta
     6001 tcgagaaaaa ccgttatcgc gctgaagaac tggcagaaga acgtattctc gacgtgctga
     6061 tcccacctgc taaaaacaac tggggacaga ccgaacagca gcaggaaccg tccgctgctc
     6121 gtcaggcatt ccgcaaaaaa ctgcgtgaag gccagcttga tgacaaagaa atcgagatcg
     6181 atcttgccgc agcaccgatg ggcgttgaaa ttatggctcc tccaggcatg gaagagatga
     6241 ccagccagct gcagtccatg ttccagaacc tgggcggtca gaagcaaaaa gcgcgtaagc
     6301 tgaaaatcaa agacgccatg aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc
     6361 cggaagagct gaagcaagac gctatcgacg ctgttgagca gcacgggatc gtgtttatcg
     6421 acgaaatcga caaaatctgt aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag
     6481 gcgttcagcg agacctgctg ccgctggtag aaggttgcac cgtttccacc aaacacggga
     6541 tggtcaaaac tgaccacatt ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt
     6601 ctgacctgat cccggaactg caaggtcgac tgccaatccg cgttgaactg caggcactga
     6661 ccaccagcga cttcgagcgt attctgaccg agccgaatgc ctctatcacc gtgcagtaca
     6721 aagcactgat ggcgactgaa ggcgtaaata tcgagtttac cgactccggt attaaacgca
     6781 tcgcggaagc ggcatggcag gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac
     6841 acactgttct ggagcgttta atggaagaga tttcctacga cgccagcgat ttaagcggtc
     6901 aaaatatcac tattgacgca gattatgtga gcaaacatct ggatgcgttg gtggcagatg
     6961 aagatctgag ccgttttatc ctataatcgc gttcaatcat tttcatcatt gtttgatggg
     7021 gctgaaaggc cccattttta ttggcgcgta ttatgactga acaacaaatt agccgaactc
     7081 aggcgtggct ggaaagttta cgacctaaaa ccctccccct cgcctttgct gcaattatcg
     7141 tcgggacggc gctggcatgg tggcaaggtc attttgatcc gctggtcgcc ctgctggcac
     7201 tgattaccgc cgggctatta cagatccttt ctaacctcgc caatgattac ggcgatgcgg
     7261 taaaaggcag cgataaacct gaccgtatcg gaccgctacg cggcatgcaa aaaggggtca
     7321 ttacccagca agagatgaaa cgggcgctca ttattaccgt cgtgctcatc tgtctctccg
     7381 ggctggcact ggttgcagtg gcgtgccata cgctggccga ttttgtcggt ttcctgattc
     7441 ttggcgggtt gtcgatcatt gccgctatca cctacaccgt gggcaatcgt ccttatggtt
     7501 atatcggtct gggtgatatt tccgtactgg ttttctttgg ctggttaagc gtcatgggga
     7561 gctggtattt acaggctcat acattgattc cggcactgat ccttccggcg accgcatgcg
     7621 gcctgctggc aacggcagta ctgaacatta ataacctgcg tgatatcaat agcgaccgcg
     7681 aaaacggcaa aaacacgctg gtggtgcgct taggtgaagt gaacgcgcgt cgttatcatg
     7741 cctgcctgct gatgggctct ctggtatgtc tggcgctgtt taatctcttt tcgctgcata
     7801 gcctgtgggg ctggctgttc ctgctggcgg caccgttgct ggtgaagcaa gcccgttatg
     7861 tgatgcggga aatggacccg gtggcgatgc gaccgatgct ggaacgcact gtcaagggag
     7921 cgttactgac taacctgctg tttgttgtag ggatattcct aagccagtgg gcagcataac
     7981 tgacaaatat caattaacaa ttgatgattt tgccaacaga ccacatagcg cgatatactg
     8041 aaaattctcg cagcaactga atgttaagcc tatgaaatac gatacttccg agctttgtga
     8101 catctatcaa gaagatgtta acgtcgtgga accgctgttc tccaactttg gcggacgggc
     8161 gtcgtttggc ggacaaataa tcacggtaaa atgtttcgag gacaacgggt tgctgtacga
     8221 tctgctcgaa cagaatggcc gtggtcgtgt tcttgtcgtc gatggcggtg gttctgttcg
     8281 tcgcgcactg gtcgatgctg aactggcgcg tctggcagta caaaatgaat gggaaggtct
     8341 ggtcatttac ggcgcggtgc gtcaggtaga tgacctggaa gagttggata tcggcatcca
     8401 ggcgatggcg gcaattccgg ttggtgccgc tggcgaaggc attggcgaaa gcgatgtccg
     8461 cgtcaatttt ggcggtgtta ccttcttctc cggcgaccat ctttatgccg acaataccgg
     8521 gattattctt tcagaagatc cgctggatat tgaataagta tctgccatgc gttaaaaggg
     8581 gaattaaatt cccctttttt atacatggac gtattc
//
LOCUS       DAZFBR010000163         8552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFBR010000163 DAZFBR010000000
VERSION     DAZFBR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.163"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..81
                     /locus_tag="ACHJZ2_004440"
     CDS             <1..81
                     /locus_tag="ACHJZ2_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4570423.1"
                     /translation="SIAVSMIVGRDNCAFDLDRGNFVLKN"
     gene            112..231
                     /locus_tag="ACHJZ2_004441"
                     /pseudo
     CDS             112..231
                     /locus_tag="ACHJZ2_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(242..490)
                     /locus_tag="ACHJZ2_004442"
     CDS             complement(242..490)
                     /locus_tag="ACHJZ2_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4570424.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESSDEWFVH"
     gene            complement(552..1649)
                     /locus_tag="ACHJZ2_004443"
     CDS             complement(552..1649)
                     /locus_tag="ACHJZ2_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4570425.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQKSRNKTS"
     gene            1738..2775
                     /gene="dusA"
                     /locus_tag="ACHJZ2_004444"
     CDS             1738..2775
                     /gene="dusA"
                     /locus_tag="ACHJZ2_004444"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO4570426.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2909..3151
                     /gene="pspG"
                     /locus_tag="ACHJZ2_004445"
     CDS             2909..3151
                     /gene="pspG"
                     /locus_tag="ACHJZ2_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO4570427.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(3317..4300)
                     /gene="qorA"
                     /locus_tag="ACHJZ2_004446"
     CDS             complement(3317..4300)
                     /gene="qorA"
                     /locus_tag="ACHJZ2_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO4570428.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            4383..5798
                     /gene="dnaB"
                     /locus_tag="ACHJZ2_004447"
     CDS             4383..5798
                     /gene="dnaB"
                     /locus_tag="ACHJZ2_004447"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4570429.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5851..6930
                     /gene="alr"
                     /locus_tag="ACHJZ2_004448"
     CDS             5851..6930
                     /gene="alr"
                     /locus_tag="ACHJZ2_004448"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO4570430.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            7183..8376
                     /gene="tyrB"
                     /locus_tag="ACHJZ2_004449"
     CDS             7183..8376
                     /gene="tyrB"
                     /locus_tag="ACHJZ2_004449"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO4570431.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         2035 a         2056 c         2182 g         2279 t
ORIGIN      
        1 tcaatcgcag tttcaatgat tgtaggaaga gataattgtg cttttgacct tgacagaggt
       61 aacttcgttt taaaaaatta agatagcaat ggcgggttaa cttttcgtaa aatgaaatta
      121 attctcggta cgacatgtaa aacgcggcac ctcgtatgca agaacgtact gcggttggct
      181 ggtgaacttt cgatagtgct agtattgaat gatttccagc cgttaccgat ttacgtgttt
      241 attagtgaac aaaccactcg tcagaagatt cccaggtatc tttcagagtc tcctgaacaa
      301 atgtttttgc agaatcctta tctgcagtgc gtgtaacaga aagcccatca ttgctggtgg
      361 cttttacgat cacttctaca tcgtcataac gcttactgat gcgccgagtt aattcttcct
      421 ttaacgcatc cacagcaccg tttggcattt tagtcatttt ctctttggct atgcagattt
      481 caatacgcat aaaaatccct ctatactgtg tttgtataca gtgttatttt tatctgtata
      541 aataaacagt gtcaagaggt cttgtttctg ctcttttgga gttcttcaaa acgattatgt
      601 aaagatttcg gatacagttc ggtatatacc tgccatagca cgtttaatga acgatgccct
      661 gtaacttgag cgacttcctc aatactaaaa ccagcctcaa ataagcgact tgccccttct
      721 ctacgcaaat catgatatcg cagatcttta atacctaatt tgcttcttac cctctgaaat
      781 cctgcggtaa cagaagtgct gttatatgga aaaatgaatt ctgatttttt tggttgtcgc
      841 tggacgatat cccaggcttc cccaagcaag gcaactttca tatggttacc ttccttttta
      901 cgtggatctt tcctatctct tactagtata gatttttgtt cttggtcgag atcttcccat
      961 cgtaaccggc atacttctcc gattcgcata caggaccaca cagaaaattt gaggatatca
     1021 acgaacggaa tttttgagca tttatgagta gatcgttgtt gaaggccttc aatgagcatg
     1081 tcaagttcat cagatgctgg tctacgatta cgacggtttg atttaccaat caaaccgagt
     1141 ttaagtagat atggacgagc gcttttcgcc gggtttgatg tgtaattgat tccgtataca
     1201 ggttttgccg catccagaac actgccaaga taactaacat cgtggctgac tgttgctggg
     1261 cctgcaccag cgttgtttct tagcctgcaa tgttcaatta cgtcattttc tgtcagttca
     1321 gatagtttga tcgcggatat gtcactatcc ataagcagtt ccagcacata tcttttagta
     1381 cggcctgctt tacctccggc atttgggtca tttaaatatt tgtgtagtaa gtcacggact
     1441 gtaagtccgt caactgcatt tgatgatgga atgccatata gatctaattc catcactttc
     1501 tgtgtgcccc atgttttggc atgagcatgt ttagggaatg ttttgctttc cctgtaagtg
     1561 ataacacctt tttctttgat aatcacatta cagcgatagc gtggtgtgcc atcggatttt
     1621 agtcgtttct ctatgttata gtacgccatt acacgacctc gttatttcgg gttcccataa
     1681 aacgtgggaa cctgtgcggg aacctaacgc gagaaaaata gcctgaaatg ttcaaaaatg
     1741 cacgataatc atgaagcaca aaaaattaat caaaccagcg tgatgcctga aaaaactggt
     1801 gtttactgga attctcggtt tagcgttgca ccaatgctcg actggacgga cagacattgc
     1861 cgctatttct tgcgtctgct ttcccgcaat acgttgctgt ataccgaaat ggtgactaca
     1921 ggggcgatta ttcacggtaa aggtgattac ctggcgtaca gtgaagaaga acatccggta
     1981 gcgttgcaac tcggcggtag cgatccggcg gcgctggcgc agtgtgcgaa gctggcagaa
     2041 gcgcgtggat atgatgagat caacctgaat gtcggctgcc cgtctgaccg ggtgcagaac
     2101 ggcatgtttg gtgcgtgtct gatgggtaat gcgcagctgg ttgccgactg cgtgaaagcg
     2161 atgcgcgatg tggtgtcgat tccggtgacg gtgaaaacgc gtattggcat cgatgaccag
     2221 gacagctatg aatttctctg cgatttcatc aacaccgttt ccggcaaagg cgagtgtgag
     2281 atgttcatca tccacgcacg taaagcctgg ctttcggggt taagcccgaa agaaaaccgt
     2341 gaaatcccgc cgctcgatta tccgcgtgtg tatcaactga agcgtgactt tccgcatctg
     2401 acgatgtcga ttaacggtgg tatcaagtcg ctggaagagg ctaaagcgca tttgcaacat
     2461 atggatggcg tgatggtcgg gcgcgaagcg tatcagaatc cgggtattct ggcggcggta
     2521 gaccgagaga tctttggttc ctcggatacc gatgccgatc cggtggcggt agtgcgcgcc
     2581 atgtatccgt acattgagcg tgaactcagc caggggacgt atcttggcca tattacccgg
     2641 catatgttgg gcttgttcca gggtattcct ggcgcgcggc agtggcggcg ttatttaagt
     2701 gaaaatgccc ataaagcggg tgcagacatt aacgtgctgg aacacgcgct caaactggtg
     2761 gcggataagc gttaactttt caccaaaaag tagtcaaatt caccacgccc tgcgcaccgt
     2821 cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt gtaatgcctg
     2881 caagagattt catatttggg agagcatcat gctggaacta ctttttgtga ttggcttttt
     2941 tgtcatgttg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc tggttgtggc
     3001 gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc tgccgtggtt
     3061 actcctggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa aagtgccgaa
     3121 atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca caacctaagg
     3181 ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg cgcctctaac
     3241 agattcatcg tgctgtaccc tacatacagc cgaactataa aaagaaaggg cttcccaggt
     3301 ggaagcccta tttcttttat ggaatcaaca ggctggaacc ttgcgtcgcg cggctttcca
     3361 gaatctcatg cgcacgctgc gcatccttca gcggatattt ctgctgctcg gcgacatcga
     3421 ccttaatcac accgctggca atcaaagaga acagttcatt actggcctcg gttaattcct
     3481 cccgcgtggt gatatagcct tgcagggaag ggcgtgtcac atacaacgag cctttctgat
     3541 tgagaatgcc taagttcaca ccggtaaccg cacctgatga gttgccaaaa ctcaccatta
     3601 agccgcggcg ttgcaggcaa tccagcgacc gttcccaggt gtctctgccc acggaatcgt
     3661 acactacgcg cactttttta ccaccggtga tctcttttaa ccgctcgacc agattctctt
     3721 cacgatagtt aataacctgc cacgcgcccg cttttagcgc gctctgcgct ttttgcgcgg
     3781 ttcctacggt gccgataagt ttcgcgccca gggcttttgc ccactggcag gcaatcaagc
     3841 caacgccgcc agctgctgcg tggaacagga actgttcatc gggtttaatt tcataggttt
     3901 tgcgcagcag ataataaacc gttaagcctt tcaggaaaga tgctgcagct tgctcaaaag
     3961 aaattgccgc aggcagaatc gccgctttat ccgcattaat gttatgcaca gagctgtaag
     4021 cgcctaacgc cgactgcgca tagactacac gatcgcctgc cttaatatgc tttacaccac
     4081 tgccgacttt actcacgatg cctgccgctt cggtacctaa tccgctgggt agtgatggcg
     4141 gcgggtaaag gccgctgcgg atatacgtgt cgataaaatt gatgccgatg gctttatttt
     4201 cgacctggat ttcattctcc gccggatcgg caggagtgaa ctctacggct tgaagtactt
     4261 ccgggccacc gtgcttgtga aattcaattc gtgttgccat gtgtcctcca gaacgtatcg
     4321 tcagggtctg cttcatatgg taaagtttcg acccattctt tatctcggta actccattca
     4381 ctatggcagg aaataaaccc ttcaacaaac agcaggctga accccgcgaa cgcgatccac
     4441 aagttgccgg gctgaaagtg cctccgcact cgatcgaagc ggagcagtcg gtgttgggcg
     4501 gtttaatgct ggataacgaa cgctgggatg atgtagccga gcgtgtggta gcagacgatt
     4561 tttacacccg cccacaccgt catatcttta ctgaaatggc gcgtttgcag gaaagcggta
     4621 gtcctatcga tctgattacc cttgcggaat cgctggaacg ccaggggcaa ctcgatagcg
     4681 tcggtggttt tgcttatctg gcagagttgt caaaaaatac gccaagtgct gcgaacatca
     4741 gttcctatgc ggacatcgtg cgtgaacgtg ccgttgtccg tgagatgatc tcggttgcga
     4801 atgagattgc cgaagctggt tttgatccgc aggggcgtac cagcgaagat ctgctggatc
     4861 tggctgaatc ccgcgtcttt aaaattgccg aaagtcgtgc gaacaaagac gaagggccga
     4921 agaacatcgc cgatgtgctc gacgcaaccg tggcgcgtat tgagcagtta tttcagcagc
     4981 cacacgatgg cgttaccggg gtaaacaccg gttatgatga tctcaacaaa aaaaccgctg
     5041 gcttgcagcc gtcggatttg atcatcgtcg ccgcgcgtcc gtcgatgggt aaaacaacat
     5101 ttgcgatgaa cctcgtcgaa aacgcggcga tgttgcagga taaaccggta cttattttct
     5161 cgctggagat gccatcagaa cagatcatga tgcgttctct ggcgtcgctg tcgcgcgttg
     5221 accagactaa aatccgtacc gggcagctcg atgatgaaga ctgggcgcgc atttccggca
     5281 ccatgggtat tttgctcgaa aaacgcaata tctatatcga tgactcctcc ggcctgacgc
     5341 caacggaagt gcgttcccgc gcacgccgta ttgcccgtga acacggcggc atcgggctta
     5401 tcatgatcga ctacctgcaa ctgatgcgcg taccggcact ttccgataac cgtacgctgg
     5461 aaattgcaga aatctcccgc tcgctgaaag cactggcgaa agaactgaac gtgccggtgg
     5521 tggcgctgtc ccagttgaac cgttctctgg aacaacgtgc cgacaaacgt ccggtcaact
     5581 ccgacctgcg tgaatctggc tctatcgagc aggatgcgga cttgatcatg tttatctatc
     5641 gtgatgaggt gtatcacgaa aacagtgatt taaaaggcat cgcggaaatt attatcggta
     5701 aacaacgtaa cggcccaatc gggacggtac gcctgacctt taacggtcaa tggtcgcgct
     5761 tcgacaacta tgcggggccg cagtacgacg acgaataata attattttat gaattaggta
     5821 attaaagcaa acacttatca aggaacacaa atgcaagcgg caactgttgt gattaaccgc
     5881 cgcgctctgc gacacaacct gcaacgtctt cgtgaactgg cacctgccag taaaatggtt
     5941 gcggtggtga aagcgaacgc ttatggtcac ggtcttcttg agaccgcgcg aacgctcccc
     6001 gatgctgacg cctttggcgt agcccgtctc gaagaagctc tgcgactgcg tgcgggggga
     6061 atcaccaaac ctgtactgtt actcgaaggc ttttttgatg ccagagatct gccgacgatt
     6121 tctgcgcaac attttcatac cgccgtgcat aacgaagaac agctggctgc gctggaagag
     6181 gctagcctgg acgagccggt taccgtctgg atgaaactcg ataccggtat gcaccgtctg
     6241 ggcgtaaggc cggaacaggc tgaggcgttt tatcatcgcc tgacccagtg caaaaacgtt
     6301 cggcagccgg tgaatatcgt cagccatttt gcgcgcgcgg atgaaccaaa atgtggcgca
     6361 accgagaaac aactcgctat ctttaatacc ttttgcgaag gcaaaccagg tcaacgttcc
     6421 attgccgcgt cgggtggcat tctgctgtgg ccacagtcgc attttgactg ggtgcgcccg
     6481 ggcatcattc tttatggcgt ctcgccgctg gaagatcgct ccaccggtgc cgattttggc
     6541 tgtcagccag tgatgtcact aacctccagc ctgattgccg tgcgtgagca taaagtcgga
     6601 gagcctgtcg gttatggtgg aacctggata agcgaacgtg atactcgtct tggtgtagtt
     6661 gcgatgggct atggcgatgg ttatccgcgc gccgcgccgt ccggtacgcc agtgctggtg
     6721 aacggtcgcg aagtgccgat tgtcgggcgc gtggcgatgg atatgatctg cgtagactta
     6781 ggtccacagg cacaggataa agccggggac ccggtcattt tgtggggtga aggtttgccc
     6841 gtagaacgta tcgctgaaat gacgaaagta agcgcttacg aacttattac gcgcctgact
     6901 tcaagggtcg cgatgaaata cgtggattaa tcgttctgta atatttgatt gtctgtgccg
     6961 gatgcggcgt gaatgcctta tccggccaat aaaatcctac caattcaata agttgatatt
     7021 ctttcatgct cttataaagg tcgtgcctct ggcggatgta cgtttgtcat gagtctcact
     7081 ctgttgctaa ttgccgttcg cccctgaaca tccactcgat cttcgccttc ttccggttta
     7141 ttgtgtttta accacctgcc cgtaaacctg gagaaccatc gcgtgtttca aaaagttgac
     7201 gcctacgctg gcgacccgat tcttacgctt atggagcgtt ttaaagaaga ccctcgcagc
     7261 gacaaagtga atttaagtat cggtctgtac tacaacgaag acggaattat tccacaattg
     7321 aaagccgtgg cggaggcgga agcgcgcctg aatgcggtgc ctcatggcgc ttcgctttat
     7381 ttaccgatgg aagggcttaa cagctatcgc catgccattg cgccgctgct gtttggtgcc
     7441 gaccatccgg tactgcaaca acagcgcgta gcaaccattc aaacccttgg cggctcaggg
     7501 gcattgaaag tgggcgcgga tttcctgaaa cgctacttcc cggaatcagg cgtctgggtc
     7561 agcgatccta cctgggaaaa ccacgtagca atattcgccg gggctggatt cgaagtaagc
     7621 acttacccct ggtatgacga agcgactaac ggcgtgcgct ttaatgacct gttggcgatg
     7681 ctgaaaacat tacctgcccg cagtattgtg ttgctgcatc catgttgcca caacccaacg
     7741 ggtgccgatc tcactaatga ccagtgggat gcggtgattg aaattctcaa agcccgcgag
     7801 cttatcccat tccttgatat tgcctatcaa ggatttggtg ccggtatgga agaggatgcc
     7861 tacgccattc gcgccattgc cagcgctgga ttacccgctc tggtgagcaa ttcgttctcg
     7921 aaaattttct ccctttacgg cgagcgcgtc ggcggacttt ctgttctgtg tgaagatgcc
     7981 gaagctgcag gccgcgtact ggggcaattg aaagcaacag ttcgccgcaa ctactccagc
     8041 ccgccgaatt ttggtgcgca ggtggtggct gcagtgctga atgacgaggc attgaaagcc
     8101 agctggctgg cggaagtaga agagatgcgt actcgcattc tggcaatgcg tcaggaactg
     8161 gtgaaggtat taagcacaga gatgccagaa cgcaatttcg attatctgct taatcagcgc
     8221 ggcatgttca gttataccgg tttaagtgcc gctcaggttg accgactacg tgaagaattt
     8281 ggtgtctatc tcatcgccag cggtcgcatg tgtgtcgccg ggttaaatac ggcaaatgtg
     8341 caacgtgtgg caaaggcgtt tgctgcggtg atgtaatgca ggaaagcagg ctggaattac
     8401 ccagcctgca gtgaaattaa actgtcgtcg ctttcactct ttctttatag atgatttttt
     8461 tgataccatc gttctacgtg agagataata aacgttgtta gttcttttat tgttaagttt
     8521 atcccaatta tctggaattc ctttccctgt tt
//
LOCUS       DAZFBR010000164         8371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFBR010000164 DAZFBR010000000
VERSION     DAZFBR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.164"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(150..226)
                     /locus_tag="ACHJZ2_004450"
     tRNA            complement(150..226)
                     /locus_tag="ACHJZ2_004450"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Asp,seq:gtc)
     gene            complement(359..1090)
                     /gene="dnaQ"
                     /locus_tag="ACHJZ2_004451"
     CDS             complement(359..1090)
                     /gene="dnaQ"
                     /locus_tag="ACHJZ2_004451"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO4570432.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1155..1622
                     /gene="rnhA"
                     /locus_tag="ACHJZ2_004452"
     CDS             1155..1622
                     /gene="rnhA"
                     /locus_tag="ACHJZ2_004452"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO4570433.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1619..2341)
                     /locus_tag="ACHJZ2_004453"
     CDS             complement(1619..2341)
                     /locus_tag="ACHJZ2_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4570434.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2375..3130
                     /gene="gloB"
                     /locus_tag="ACHJZ2_004454"
     CDS             2375..3130
                     /gene="gloB"
                     /locus_tag="ACHJZ2_004454"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO4570435.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3202..4560
                     /gene="mltD"
                     /locus_tag="ACHJZ2_004455"
     CDS             3202..4560
                     /gene="mltD"
                     /locus_tag="ACHJZ2_004455"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO4570436.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(4608..5378)
                     /locus_tag="ACHJZ2_004456"
     CDS             complement(4608..5378)
                     /locus_tag="ACHJZ2_004456"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4570437.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(5456..6256)
                     /locus_tag="ACHJZ2_004457"
     CDS             complement(5456..6256)
                     /locus_tag="ACHJZ2_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4570438.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6497..7411
                     /gene="yafC"
                     /locus_tag="ACHJZ2_004458"
     CDS             6497..7411
                     /gene="yafC"
                     /locus_tag="ACHJZ2_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO4570439.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7408..8211)
                     /gene="dkgB"
                     /locus_tag="ACHJZ2_004459"
     CDS             complement(7408..8211)
                     /gene="dkgB"
                     /locus_tag="ACHJZ2_004459"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO4570440.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2232 a         1999 c         2044 g         2096 t
ORIGIN      
        1 gaattttaat gaatgagaca acaagcgaga atcacgcact tacgccagag taaaatcatt
       61 gaacagagaa atggtggaat tcaaatacaa aaaaaaccgc aaaattaaaa atcttgcggt
      121 tctctgaact cattttcatg aggtgaattt ggcggaacgg acgggactcg aacccgcgac
      181 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccgcgt tgtgttccgt
      241 tgggaacgag gcgaatatta cggattgcct cacccttcgt caacggtttt tctcatcttt
      301 tgaatcattt gctgcaaaaa tcgcccaagt cgctattttt agcgcctttt acatgtattt
      361 atgcccgcca gaggcagctt ccgccctttt tctgcaccag atcgagacgg gcttcatgtg
      421 ctgcaagctc ttcatctgtc gcaaaaacaa cgcgtaactt acttgcctga cgtacaaggc
      481 gctggattgt tgcttcacct tgttgctgtt gtgtctctcc ttccatcgca aaagccatcg
      541 acgtttgacc accggtcatc gccagataaa cttccgcaag gatctgggca tcgagtaatg
      601 ccccgtgcag cgttcgttta ctgttatcta tttcgtagcg ggcacataac gcatcgaggc
      661 tgttgcgttt accgggaaac attttcctcg ccaccgcaag gctatcggtg accttacaga
      721 aggtattagt cttcggaata tcgcgcttaa gcaacgagaa ctcgtagtcc ataaagccga
      781 tatcgaacgc tgcgttatgg atcaccaact ccgcgccgcg gatatagtcc atgaactcat
      841 cggctacttc ggcaaacgtg ggcttatcga gcaaaaattc atcggcaata ccatgtacgc
      901 caaaggcttc cggatccacc agccgatcgg gtttgagata aacatggaag ttattgcccg
      961 tcaggcgacg gttcaccact tcaacggcac caatctcaat gatcttgtgg ccttcatagt
     1021 gcgcaccaat ctggttcata ccggtggttt cggtatcgag aacgatctgg cgtgtaattg
     1081 cagtgctcat agcggtcatt tatgtcagac ttgtcgcttt acagttcgat tcaattacag
     1141 gaagtctacc agagatgctt aaacaggtag aaattttcac cgatggttcg tgtctgggta
     1201 atcctggacc tgggggttac ggcgctattt tacgctatcg cggacgcgag aaaaccttta
     1261 gcgcaggcta cacccgcacc accaacaacc gtatggagtt gatggccgct attgtcgcgc
     1321 tggaggcgtt aaaagaacat tgcgaagtca ttttgagtac cgacagccag tatgtccgcc
     1381 agggtatcac ccagtggatc cataactgga aaaaacgtgg ctggaaaacc gcagacaaaa
     1441 aaccagtaaa aaatgtcgat ctctggcaac gtcttgatgc tgcattgggg cagcatcaaa
     1501 tcaaatggga atgggttaaa ggccatgccg gacacccgga aaacgaacgc tgtgatgaac
     1561 tggctcgtgc cgcggcgatg aatcccacac tggaagatac gggctaccaa gtcgaagttt
     1621 aagcctgtgg tttacgacat tgccgggtgg ctccaaccgc ctggcgaatt cgtggcttgt
     1681 ttttactctg tttcatcgga tttagcgtta aaggaatagt ccgtttccgg gcaacaataa
     1741 gttgtaagca accaagcgca ggaatatgcg cattcaaaag ttttcctccg tgtttgttcc
     1801 acgggagaac gtggaaacgg ctggcgtgta gcacttcaaa attcaacaaa gagagccagt
     1861 ccagttgccg catcagagta aacatccggc tgttataggg cgaggttttg cgcaataccg
     1921 gcacaagttt gcgtaatccc attaaactaa tgggattgaa gccactaatg accagccagc
     1981 catcatcaat caatacccga tcggcttcac gcaataaacg atgcggatcg gtgcaccacg
     2041 gcaatgtatg tgccagtaga caaacatcaa cggatttatc ggcaaaagga agatgaagtg
     2101 ggtccgcctg cacctggaca ggcattcctt gcgcagaaac attcacttga tgagaaaccg
     2161 cgcacgcttc gcaattgatt tctgcgctta aattgccaat cttaagcaga taaaaaccat
     2221 acattttagt gaaccacggg ttgagctggc gctccagcgc cttgcgataa agctctcccc
     2281 agggcaaatc gccccagcga tcaggagcca cgacagtttg agggactctt gccggtttca
     2341 tcacaacctt ccgtttcaca ctgagaggta atctatgaat cttaacagta ttcccgcctt
     2401 tgatgacaat tacatctggg ttttgaatga tgaagcaggt cgctgcctga ttgtcgatcc
     2461 cggagacgca gagccagtat taaacgccat tgccgccaat aactggcaac cggaggccat
     2521 atttctcacc caccatcatc acgatcacgt tggcggcgta aaagaactgg tggaaaagtt
     2581 tccgcaaatt gtggtgtatg gtccacaaga gacacaagat aagggaacaa cacaggtagt
     2641 caaagatggc gaaactgcct tcgttttggg gcatgaattt agtgtaattg ccacgccggg
     2701 tcacacttta ggacatatct gttacttcag taaaccttat ctattttgcg gcgacacgct
     2761 gttttctggt gggtgtggtc ggttgtttga agggacagca tcacaaatgt atcaatcact
     2821 taaaaagtta agtgcgttac ctgacgatac attggtatgt tgtgctcatg aatatacgtt
     2881 atcaaatatg aagtttgctt tgagtattct tccgcacgat ttgtccataa atgattatta
     2941 tcgtaaagtt aaggagttac gggcaaaaaa tcaaataaca ctacccgtaa ttctgaaaaa
     3001 tgagcggcaa attaatgttt ttttaagaac ggaagatatt gatttaatta atgtaattaa
     3061 tgaagaaaca ttattgcaac aacctgaaga gcgttttgca tggttaaggt caaagaaaga
     3121 taggttctga taaaactttc ttgtcatcgg ctccgttcgc cgttatgatc ggtcgtcttt
     3181 taagcaacta ttgacgcaca catgaaggca aaagcgatat tactcgcctc tgtcctgctc
     3241 gtgggttgcc agagtaccgg caacgttcaa cagcacgcac agagcctttc tgcagctggt
     3301 caaggggaag cagcaaagtt tacaagtcag gcacgatgga tggacgatgg gacgtctatc
     3361 gcgccagatg gtgacttgtg ggctttcatt ggcgacgagc taaagatggg aattccggaa
     3421 aatgaccgga ttcgcgaaca gaaacagaaa tatttacgca ataagagcta tctccacgat
     3481 gtaactttac gggcagagcc gtatatgtac tggatagccg ggcaagttaa aaaacgtaac
     3541 atgcctatgg aactggtact actacccata gtggagagcg cttttgatcc tcacgcaacg
     3601 tctggcgcca atgccgcagg catctggcag atcattccga gcacggggcg caattatggt
     3661 ttgaaacaga cccgcaatta tgacgcgcgt cgcgatgttg ttgcttcaac aactgccgcg
     3721 ctgaatatga tgcagcgtct gaacaaaatg tttgatggcg actggcttct gaccgtagcg
     3781 gcttataaca gcggcgaagg tcgggtcatg aaggcaatta aaacgaacaa agcgcgtggg
     3841 aaatccacgg acttctggtc gttaccgttg ccgcaggaaa cgaagcagta cgtgcctaaa
     3901 atgctggcat tgagtgatat tctcaaaaac agcaagcgtt atggcgtacg tctgccaacg
     3961 accgatgaaa gccgtgctct ggcgcgtgtg cacctgagta gcccggttga aatggcgaag
     4021 gttgcagata tggcggggat ttccgtcagc aagctgaaga cattcaacgc tggcgtgaaa
     4081 ggctccacgc tgggcgcaag tggtccgcag tacgtgatgg tgccaaagaa gcatgcagat
     4141 caactgcgtg aatctctggc ttcaggcgaa attgctgctg tacagtcgac gctggttgcc
     4201 gacaatacgc cgcttaacag ccgtgtttac accgtacgct ctggcgacac gctttcaagt
     4261 atcgcttcac gtctcggcgt aagcaccaaa gatttgcagc agtggaacaa actgcgcggc
     4321 tctaagctga aaccaggcca aagtctgacg attggcgcag gtagtagcgc acagcggttg
     4381 gcaaacaaca gcgatagcat tacgtatcgt gtgcgcaaag gcgattcgct ttcaagcatt
     4441 gctaaacgcc acggcgtgaa catcaaagat gtgatgcgct ggaacagcga tactgcgaat
     4501 ctgcaaccag gcgataagct gacgttgttt gtgaaaaaca acagcatgcc agactcctga
     4561 caaaccagat aatagaaagg caccgattcc cccggtgcct tttttattta tgccgcttta
     4621 tgtgcatcta ccatgatgat atcactggtg aaagagccat cattctgcaa ggcaaaatac
     4681 gttctcacct ctgtcgatgc gctctgttgg taaatgcgaa tagcgtctac taacgcttct
     4741 ggcgtacgca ttctcgcgac ccatgaagaa aattccagcg gtaacttatc tgtaattaaa
     4801 ttatcaacta tcagattggc ttcattgatt aacctcaacc actcaccgct ggcgtagttt
     4861 cgtacgtgag aggtatcgca taatgcttct accgtctgta accagatgtc gcgcactggg
     4921 tggcccggag acattacgtc catcacaatc agcctaccgc caggtttcaa tatccggttc
     4981 acttcacgca gtgctgcacc aacatcatgc cagtgatggg cagaataacg gctgataaca
     5041 atatcaaatg cgttatcggc aaatggcaga ctttcggcat acccctggcg ggtggtgata
     5101 tttttcagtt gccggacttc ggcagcttgt gccacgacat ccagcatttg ggcagataag
     5161 tcatacgcca ccaccgtgct cacattttgc gcagcgacaa agctggcatg ccctgctccg
     5221 cagcccatat caagtacact tgcatcagga taatcagcca gacacaccgc caggagctgt
     5281 aaatctcgtc cggatgcatg tacggtactg gtcagatatt cgctggcttg agagctaaac
     5341 tgcttttcta cgtggtcatg gtgggattgt gttgtcattg cactgtcctt ttgttgtttg
     5401 agtataaaag ggcagctcca aacgttggtc tgccctgtgg cagacctgac ataccttatt
     5461 tatcaggctt gccgggactg aattcaacga gtagcggatt gtgatcggac gcgcgcgtaa
     5521 ccagtacaga agcttcgctg acgttcagac cacggtagaa aacaaaatcg agcgggcgac
     5581 caaacgcccg acggcgctga tcatcggtaa aacgcacctg acgcagcgac atttcccgcg
     5641 caaaacgata taacgcgttc atccttctac ggctccaagc attgaaatct cccgccataa
     5701 tgaccgggcc gctgtggtga gctatctgat caccaatagg aagtaactgc ttactataaa
     5761 catccacgcc cagactgaag ttgacggcgt gtatattaac caccattaac aggcgggtgt
     5821 caggtaatgg atagaccgtc accagtgccg acttcgccag acgcaaaatg ggttctcgtt
     5881 cacgtaacgg gcagcaatac actggatgtg ccgccgaaag ggtcattacg ccagaaggat
     5941 gttgtggcag cacgaaagcg ggtacctgat cggcggcaag atagttagca gtcgcaaact
     6001 gtaccaactc tggcgttgtt tgcgcttcct gcaataacac cagatgtgcg tctttgccgt
     6061 aattctttaa taccgacaac cattcagcgc gttgctgttt gtatatgttc cacaccagga
     6121 tccgaatacg ctcttcggta cttaacggtt ctccgggtgg taatgcctgg ccgatgctcg
     6181 caaaagaccc cggcggtaag atcctctccg caggttgtcc ggcaacatag cgcatggcat
     6241 aggtgttttt tcgcactttt gactttgaaa cctctattac gtcaaccctt ccgcactcgg
     6301 gagggttcta ctgttatagg gattttagct cactctttca acgagcaatt gtatattgtt
     6361 atgtaagcaa gtgcttacac cggtcagaat tttgctcatt attgagaaca ttaaatcgga
     6421 tgacatatca cgcatcctga tggtttaatg acaaataaat tcaaaatatc tcatctattt
     6481 tgcaacagga gcaaaaatga aagccacgtc ggaagaactc gccatttttg tttcggtcgt
     6541 cgaaagcggc agctttagcc gggcagcgga acaattaggg caagcaaact cagcggtaag
     6601 ccgggcggtg aaaaagctgg agatgaaact tggcgttagc ctgcttaatc ggaccacgcg
     6661 gcaacttagc ctgacggaag aaggcgagcg ttattttcgt cgcgtacagt caattttgca
     6721 ggagatggca gcggcagaat cagaaattat ggagacgcgt aatacaccgc gtggactgtt
     6781 gcgcatcgat gccgcaactc cagtggtgct gcactttctg atgccgttga ttaagccttt
     6841 tcgtgaacgc tatccggaag tcactttatc gctagtctcc tccgaaacga ttattaattt
     6901 gatcgaaaga aaagtggatg tcgcgatacg cgccggtacg ttaacggatt ccagcttacg
     6961 tgccaggccg ttatttaaca gttatcgaaa aattatcgcc tcccccgatt atatttcccg
     7021 ctacgggaag ccagaaacga tcgacgattt aaagcaacat gtttgcctgg gattcactga
     7081 acccgcttcc ctcaatacct ggccggtagc ctgtagcgat ggacaattac atgaggtgaa
     7141 gtacggtttg tcatccaata gtggggaaac actgaaacag ctttgcctga gtggcaacgg
     7201 gattgcgtgt ttgtccgact atatgatcga cagagaaatc gctcgcggag aattggtgga
     7261 gttaatggca gataaagtgt tgccagtgga aatgccattc agtgccgtct attacagcga
     7321 ccgtgcggta agtacgcgca tccgggcttt tatcgatttc cttagcgagc atgtaaaaac
     7381 agctcccgga ggagctgtca gagaggctta atcccattca ggagccagac cttccgggct
     7441 aaccaggcgg tcgttgcaat ccagtgcggc gatcgctttt ttatcttcgg catcaagctg
     7501 taaattttgt gccttaagat tactctccag gttttcacgt ttagtagaag aaggaattac
     7561 tgagtaacct tcccccatag cccacgccag aatcacttgt gccggagtcg cattgtgttt
     7621 agctgcgata cgagcaataa cctcatcttt cagggcctta ccatacgcca gcgtcatata
     7681 ggaagtaata tggatgccgt gctgtttagc ccaggcaacc actttacggt tttgcagata
     7741 aggagagagt tcaatctggt tagtagcgat gttttcagcg ccaacagcag caatcgcctt
     7801 ttccatcaat gggatcgtga agttggaaat accgatctca cgcgtcaacc cttctttttt
     7861 ggcttccagc agcgcctgca taaactcttc aacagagact tcatcgtttg gtgacggcca
     7921 gtggattagc gtcagatcaa cataatcggt acgcaatttt tgcaggctct cttttagact
     7981 cgggatcaat ttgtctttgc tgagattttc aatccagatt ttagtggtga tgtagagttc
     8041 atgacgtggc acgccacttt ctgcaatcgc cagacctact gcggcttcgt tatcatagat
     8101 ttgtgcagta tcaattgcgc gataaccaag ttcaagcgcc gttttcacag atgaaataac
     8161 aacgtcgtct ttcagacgga aagtacctaa accaaatgca gggatagcca taatatgcct
     8221 cttttaatgc tattttcgtt aactgaggtg attatgggct ttagaatgtt gcggaaaaag
     8281 ggcgtaaaac gcagaggatt tttgcgattt tagcaataat agaaatgtag acgaaaaaaa
     8341 acctcgagcg ataactcgag gcttcttaat a
//
LOCUS       DAZFBR010000165         8335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFBR010000165 DAZFBR010000000
VERSION     DAZFBR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.165"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..2132)
                     /locus_tag="ACHJZ2_004460"
     CDS             complement(<1..2132)
                     /locus_tag="ACHJZ2_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO4570441.1"
                     /translation="MSTDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVLRISRTPSGANAGGGILTDRGEGFVHDDASVE
                     RDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKE
                     GKQDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAE
                     LHEARKNIPPQNPVDADKLLAASRGEFVEGISDPNDPKWIKGIQTRDSVYQNQPETEQ
                     NDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQRSGGNCPDCGAVMGDATYQ
                     ETFDDKNLVEVQEDDSEKMEGAEHQHKENTGGNQHHASDSETGETADPLITVNGHRII
                     TSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVID
                     RDTIKWWLKQSREAQSAIMTDEIPLDDAL"
     gene            complement(2225..2413)
                     /locus_tag="ACHJZ2_004461"
     CDS             complement(2225..2413)
                     /locus_tag="ACHJZ2_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4570442.1"
                     /translation="MSSAFALMMTVFLITGEPQNVITGIYASKESCHQARDEQKISGE
                     CLPLNKVSLYLNNEIPAG"
     gene            complement(2410..2598)
                     /gene="dicB"
                     /locus_tag="ACHJZ2_004462"
     CDS             complement(2410..2598)
                     /gene="dicB"
                     /locus_tag="ACHJZ2_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4570443.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLELGIFGGQQ"
     gene            complement(3086..3238)
                     /locus_tag="ACHJZ2_004463"
     CDS             complement(3086..3238)
                     /locus_tag="ACHJZ2_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4570444.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAAAHRW
                     LARNAN"
     gene            complement(3408..3791)
                     /locus_tag="ACHJZ2_004464"
     CDS             complement(3408..3791)
                     /locus_tag="ACHJZ2_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4570445.1"
                     /translation="MRKKQYDTPLAERLDTISQQHHLSGSDLARIAGVGRSSVNAWKK
                     RGTISKDSAAKIAEATNVSLSWLLTGKEDTNREALDDDEKALLDVYRNLPPVERRNML
                     AAFQMRLQKLTEFYSEYVDPITRQK"
     gene            3900..4175
                     /locus_tag="ACHJZ2_004465"
     CDS             3900..4175
                     /locus_tag="ACHJZ2_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4570446.1"
                     /translation="MTALDKAIKIAGGIRPLGRAIGAWPSQIHKWANEYNGRVPTGER
                     VRQIYIATGVTPHELRPDLYPNPTDGLPAGDKANTQNTPELIHENQA"
     gene            4159..4584
                     /locus_tag="ACHJZ2_004466"
     CDS             4159..4584
                     /locus_tag="ACHJZ2_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4570447.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            4607..5560
                     /locus_tag="ACHJZ2_004467"
     CDS             4607..5560
                     /locus_tag="ACHJZ2_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4570448.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     EAYKPKRKISQAKNDPSNIAPSTVGPSNVDGSTVDKKLRIRGAMIDPDPSVLKPDPSD
                     KRSSCPDVSLPDEKQSSPVERFLEKHPDAHTWNVPKRQWGTREDLTCAQWIWGRVVAL
                     YEQAASDDGEVSRPREPNWTTWANDVRMMRMLDGRSHRQICEMFGRVQRDSFWVKNIM
                     SPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            5567..6307
                     /locus_tag="ACHJZ2_004468"
     CDS             5567..6307
                     /locus_tag="ACHJZ2_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4570449.1"
                     /translation="MKNITSGGVLARISRFVPQDAIPPYRTVAEWREWQLAEGRKRSE
                     EVNRLNHQTRVEKIINRSGIQPLHRKCTFCNYRVQNAGQRHALSQAKSIAAELEGGCT
                     NFVFSGRPGTGKNHLAAAIGNRLMEKGRSVIIITVSDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASLLSVGMLTNLNHAVMNTLLGERV
                     MDRMSMNGGRWVTFNWESWRPNVSQHRN"
     gene            6337..7107
                     /locus_tag="ACHJZ2_004469"
     CDS             6337..7107
                     /locus_tag="ACHJZ2_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4570450.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNSVVD
                     KTGHTWFLAGEGESGVTEERPVKSEAQDMLTREVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASALAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEENKTETVAVTVQSQPLFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKHRDIVRQIVDSSGRIVSEK"
     gene            7123..7518
                     /locus_tag="ACHJZ2_004470"
     CDS             7123..7518
                     /locus_tag="ACHJZ2_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4570451.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKTTDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            7575..7931
                     /locus_tag="ACHJZ2_004471"
     CDS             7575..7931
                     /locus_tag="ACHJZ2_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570452.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERAYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRILRQEERERRRNIEKLIQIE
                     RNVKARIRRVTKSRTE"
     gene            7980..8192
                     /locus_tag="ACHJZ2_004472"
     CDS             7980..8192
                     /locus_tag="ACHJZ2_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570453.1"
                     /translation="MSNAQKVINAEKYNEWVKKFSEQIFKITGDENAAKNELEPWTPE
                     GVDPNYCWWDVDPVDAANEAMSYHND"
BASE COUNT         2122 a         1842 c         2232 g         2139 t
ORIGIN      
        1 agtgcatcat ctaacgggat ttcatcggtc ataatggcag attgcgcttc gcgtgattgc
       61 ttaagccacc atttaatggt gtcccgatca atgactccgc cagcagtttc cagatcgata
      121 gtcttactaa attccggtcc catatctccg gtttgcggat cgaaaaatat tgcacctatt
      181 gagataatcg gggcatcagg attttttccc atggtttcaa ggtcgatcat tagatggtca
      241 cacgtcctgc tggtggatgt gataatgcga tgaccgttca ccgtaattaa gggatctgcc
      301 gtctcgccag tttcactatc gctggcatga tgctgattgc cgccagtgtt ctccttgtgc
      361 tgatgttcag cgccttccat tttctccgaa tcgtcttcct gaacttcaac caggttcttg
      421 tcatcgaatg tttcctggta tgttgcgtcg cccatcaccg cgccacaatc agggcagttg
      481 ccgccactcc tctgaccgca ggcggtgcag atcttttccg gttcctgttg cactactggt
      541 tcaggttgtt tcgtttctgg ctcgttttgt tgcgtatttg ggctgttctg ttccgctttc
      601 tggtcgttct gttccgtttc tggctgattc tggtacacag aatcgcgggt ctggatcccc
      661 ttaatccatt tcggatcatt cgggtcgcta atcccttcaa caaattctcc gcgagaggca
      721 gccagtaatt tgtctgcatc gacaggattt tggggcggaa tgtttttccg ggcttcatgg
      781 agttctgccc gcagtttctg atatttcgca tcaacagaat ttatctgtgg ctgagcatcc
      841 atcgactgcg tgtcctgatt atgttcagtt gtatccggtt ccactgcttc agtcgttgcc
      901 tgttcatctg ccattgcgcc agatggctgc ggtttttctt catcatcctg ttttccttct
      961 tttgttacac gctgcggcat tggagcagag gagcgaccgc aggcaatatc cacgatttct
     1021 ggatcagggt tggcatggtc ggtttcggtc agtactttgt tcagatattc ggtaacacgg
     1081 tggggagtag cctcgatacc aattggtgct tctttcacgg acgcaaccac tatcgcgcgg
     1141 gaataatcca ggtgaccagg catggcgatg aatttgtcac gaaaaacaga aaagggcggc
     1201 ttattctctg aaacgatttc ttcaatgcgt ttggcgtgtg ccggatgtgg attgtaaata
     1261 tcgatgtcca ttgaacgggc cagaacgcca gcggcaacgt cacgttccac tgacgcatca
     1321 tcgtggacaa aaccttcacc gcgatcggta agaattcctc cgccagcatt agcgccggat
     1381 ggcgtgcggc tgatgcgtaa aacacgattt cctttcatcc actcttttgt cagcagacct
     1441 cgatcggtat agtcagcgtc caggtatgct tcgaaaaaag cagtcattaa ccccagactg
     1501 gaattaacgg gattagggaa aactttgtct gtgtcgcgta ccagtttgtg aaggtcgcga
     1561 atctccagcg ggtcgagcag ctttgtgttg tgggaaatag ccagggcagt aacagcaggc
     1621 agttcttcag ctcgtgcgat atgtaatgcc tgaagttctt cccgtgaaac gtgcgttacc
     1681 ggtttttcgc tgccatgttg cgcaagccag cgaatgggca gttcctgacc ggaaatcggc
     1741 aggagcatat tatcctcaat ctcagccatg tcttcgccat tgacgttggt attgtcagtg
     1801 ctggctggtt tgtcctgaac tgagggggaa ggcgctataa atatcattgt gatgccatct
     1861 tctccgcctt tttcatagcg gttgcagaat tcagtatcga acacaccttc aggcggcagg
     1921 tcattaacaa tgggcaaatt cacgcgaaca ggttttttga aatcctcttc gtcaaatcca
     1981 gcatcgtcca ttgcgacaac accccgtgat attgcaaccg ataatttttt tgctgtgcgc
     2041 cagtaaaaac cgcctttaat cccaaggcgt tttctgactt tgtcattttt ggcttcgtaa
     2101 tacagtgcaa tttcttcttt atcagtgctc attgataaac ctcataacta ttttaaggtt
     2161 gtacgaatcc ctgctattgc tggcatgctt aatcaacggg tatggcgtta atacggctgg
     2221 cgggttatcc agccggtatt tcgttattca ggtacagcga tactttgttt aacgggagac
     2281 attcaccgga aattttttgc tcgtctcttg cctgatggca ggattcttta ctggcataaa
     2341 ttccggtaat cacattctgt ggctcacctg ttataagaaa aaccgtcatc atcagtgcaa
     2401 acgctgaact cactgctgcc ctccgaaaat gccaagttca agaagggcaa ttctggagag
     2461 tatggaatta tcattgagaa gataaggctc atattttctc atcctgatgg cgtcttcagt
     2521 aaactcccgg ttactgagca gaataccaat attaaaacac ccttcagacg tattaacgtt
     2581 tggtagtgac gtttccatta tcgcgtcctc aacaatgaat tttgtgatgc ggtgcctggt
     2641 gcctccaggt gacgttaacc agttaacaat taacgccgga tacagagaat ccacccataa
     2701 cactgttttt ggttttaact gttccgcgtg cgctcagccg cattcaccac atcacaaaat
     2761 tcactttaaa aagggcggca gagcagtcac ggagtaaaac tgataccgcc aaacgtcacc
     2821 agaaaattga taacagaggg cgttgcagcg gggttgtcac ttaagcgtat ggtcagcctg
     2881 acaacccggt gtcctcaagg ggaaggaata accccgccat acttaccgcc gcgccatttc
     2941 gcggattgcc acaaccagaa gcgcacggtc gaagaaattt aacggcaaga cttatatgca
     3001 aagggatctc gccgtgcgct ttcgtgttat gccctgactt ttcagggtaa attaacctgt
     3061 ggaaacctgt ttttactggc gctaatcagt tagcgtttct ggctaaccag cgatgcgcgg
     3121 cagcttcggt tttaaacgtt ttacttttgg tatacgtcat cgcggtaaac gtgccgtcct
     3181 gattgggaaa cacgccacat accagagatt cgttgttgcc aagattgagc gtatccatgt
     3241 tgacctcatt tgcccttaac gccggggggg gcggaactaa gacctgtcgc accgttgtgc
     3301 tttgatggga tatattgttc tcttaagatg aacatatgtc aacattgtga atgcgcaaga
     3361 ggcaaaaaaa accgccttcc ggcggtattg ttttgtaaaa taaagaatta tttttgtcgc
     3421 gttattgggt caacgtattc tgagtaaaat tcggtcagtt tttgaaggcg catttgaaaa
     3481 gctgccagca tatttctacg ctctacaggt ggcaggtttc tgtaaacatc aagcagggct
     3541 ttctcatcat catcaagcgc ctctctgttt gtatcttctt ttcctgtcag tagccaggaa
     3601 agggaaacat ttgtcgcttc tgcaattttg gctgcggaat ctttactgat tgtccctctt
     3661 tttttccagg cgttgactga tgagcgtcct acaccagcga tgcgcgctaa atctgaaccg
     3721 cttaaatgat gctgttgcga gatcgtatct aacctttctg ccagcggggt gtcgtattgc
     3781 ttttttctca tgtccattta gcaattataa ccgttcagtg aacactaaca attcctcgta
     3841 gtgttgactt atgttctctt taagtgaaca atgttgtggc tgattgaatt ggaggttgta
     3901 tgacagcatt ggacaaggca ataaagattg ctggtggtat tcgtccgcta gggcgtgcaa
     3961 taggggcttg gccgtctcaa atacacaaat gggcaaatga atacaatggt cgagtgccga
     4021 caggggagcg ggttcgtcaa atttatattg caactggggt gactcctcat gaattgcgac
     4081 cagatttata tccgaatcca accgacgggt tgcctgctgg agataaggct aacacacaaa
     4141 atacaccgga gttgattcat gaaaatcaag catgaacaca tccgcatggc gatgaatgcc
     4201 tgggcgcgtc ctgatggcga aaaagttcca gcagctggaa taacccaggc ttattttgag
     4261 ttgggtatga cgttcccaga actgtatgac gacagccatc cggaagccct ggctcgcaat
     4321 acccagaaaa ttttccgctg ggtagagaaa gacacccctg atgctgttga aaaaatgcag
     4381 gctctgttac cggcgatcga aaaggcgatg ccgcctttgc tggtggcccg tatgcgcagc
     4441 cacagttctg aatattaccg tgagatcgtc gaacggaggg atcggctggt gaaggatgtc
     4501 gatgattttg ttgcgtcagc ggttgttttg tatgaccaga tgaatcgcgg cggcccggca
     4561 gggaatgctg tggtgatgca ctaaaagcac ggtgttcggg ggttttatga gcagcaagct
     4621 tcatggtctt gtctgggaag ggtgcgcctt caccggcatg atcttatcca gggtggcggt
     4681 tatggcccgt cttgcagact acagcaatga cgagggcgtg tcatggcctg ccattgaaac
     4741 tatccggcgt cagatcggtg caagaagtga atccacagtg aaatcggcta ttgcagaact
     4801 ggcgaaagag ggctggctga cgaaggaaga gcgtaaggtc ggtgggcgta acgtaagcaa
     4861 tatctatcgg cttaatgtgg aaaaacttga agcagctgca gcggcggcgc gtgaggcata
     4921 taaaccaaaa agaaaaatta gccaggcaaa aaatgacccg tcaaatattg ccccctcaac
     4981 ggttggcccg tcaaatgttg atggatcaac tgttgataaa aaactgcgga ttaggggggc
     5041 gatgattgac cccgatccgt cagtattaaa acctgatccg tcagataaaa gatcttcttg
     5101 tccggacgtt tcactgccgg acgaaaaaca atcatcacca gttgagcgat ttctggagaa
     5161 acacccggat gcgcatacct ggaatgtacc gaagcgacag tggggaaccc gggaggattt
     5221 gacgtgtgca cagtggatct ggggacgggt tgttgcgttg tatgaacagg ccgccagtga
     5281 tgatggggag gtatcacgcc ccagagagcc taactggacg acctgggcga atgatgtgcg
     5341 catgatgcgt atgctggatg gacgtagtca ccgacagatt tgtgaaatgt ttgggcgtgt
     5401 tcaacgggat tcgttctggg taaaaaacat catgagtccg gcaaaactcc gggaaaaatg
     5461 ggatgagctg gttattcgcc tggggcgttc gcccgcgcag cgttgcgtga atcatatttc
     5521 tgaaccggat accgaaattc cgccggggtt cagggggtag cgcatcatga aaaacattac
     5581 gtcaggtggt gttctggcaa gaatcagcag atttgtgccg caggatgcaa tccctccgta
     5641 ccgtacggtg gcggagtggc gggaatggca gcttgctgaa gggcgtaagc gaagcgagga
     5701 ggttaatcgt ctgaatcatc agacgcgggt tgaaaaaatc attaaccgct ccggtatcca
     5761 gccgcttcac cggaagtgta cgttctgtaa ctaccgggtg cagaacgctg gtcagcgcca
     5821 tgccctgagt caggcgaaat ccattgcggc agagctggaa ggcggctgta cgaattttgt
     5881 gttcagtggc cggcctggca caggaaaaaa ccacctggcg gcggcgatag gcaatcggct
     5941 gatggagaag gggcgcagcg taattatcat cactgtgtct gatgtcatga gtgtgttgca
     6001 cgacagttac gacaacggca aatcaggtga aaaattttta caggagcttt gcggggtgga
     6061 tttgctggtc ctggatgaaa ttggcatgca gcgggatacg cgtaatgagc aggtcacgct
     6121 taatcagatt gttgaccgca gaacggcatc gttactcagt gtggggatgc tgacaaatct
     6181 taatcatgca gtgatgaata cacttctcgg cgagcgggtg atggaccgca tgtccatgaa
     6241 cggaggtcgc tgggtgacgt ttaactggga gagctggcgt ccgaacgtca gccagcacag
     6301 gaactgagaa gtaattttta tccggaggaa attttaatgg aaaccgtatt gcatgcactg
     6361 aaagcgatgg gtaaagccaa ttctgttgaa ctggcggcgc ggcttgatat cagccgtgaa
     6421 gaagttctca acgaactgtg ggaactcaaa aaaaatagcg ttgttgataa aacgggtcac
     6481 acctggtttc tggctggcga aggtgaatcc ggggtaaccg aagagcggcc agtaaaatct
     6541 gaagcacagg atatgctgac cagggaggtc gaacaaaaag ttaccgcaga catgatgatt
     6601 gagtttatcg gtcaggatgg ggctaaaacg tgtgaggaac tggcgggtaa gttcggtgtc
     6661 agtactcgca aggttgcttc cgcgctggcg gtggtaaccg caacggggcg gctggcacgc
     6721 gttaatcaga acggtaaatt tcgttactgc atgccgggcg ataatttacc agcagagccg
     6781 aaagccgcgc tggtaacgga aagtgatggt aaggcctttc ctcagccagc aggtgctgcg
     6841 ttaccagtcc gggaagccgc aacacaggaa gaaaataaaa cagaaactgt ggcggtcaca
     6901 gtgcagtcac agccgttgtt caccagaaaa catccggatg gtctgatttt accatcgctg
     6961 catgtggcta accgcgagct gcgccgggca aaaggtcagg ttcagaagtg ggagcgtgtc
     7021 tgcgccgcgc tgcgggagct gaacaagcac cgggatattg ttcggcagat tgtcgattca
     7081 tccggtcgta ttgtgtcgga aaagtgattg ccggaggcgc ttatggcgaa accttttaca
     7141 cacgaacagc gtgaagaact gaaggcccga attatcgggc tggtacgcaa aaatgaacgc
     7201 atgacgatat cacaactgga gagagcgacg ggagcaggct ggcattcagt cagacgttgc
     7261 cttgtggatg tactggcttg cggcgattta tacatgcccg gtaaatacgg tgtttttaca
     7321 tcagaacagg tgtatcgcgt atggcgtaag gcagcggaga aaacaaccga ccagacattg
     7381 attcgaaagt taccagacgg agaaatacgc cgctacgaca gacaacagaa cataatctgt
     7441 ggcgagtgcc ggaagagtga agttatgctg cgtgtactgg cgttctatca gggcaatttt
     7501 caggaggcgg tattgtgagt gaattagcta tcaggcttca attgtcgctg gcattcacat
     7561 caaaggagaa gagcatggaa ataaaaccag aagatgagtt aagcaatatc gttttatttc
     7621 cggtaaaaga ggatgaccca cgtaatcagg ttaattttct ttatgagcca tcggaaagag
     7681 catattgtca tcacgcctct gttcgggttg acgaaaaaga gcgtcaggtc cgctgtaaaa
     7741 tctgcggtgc agttgtggag ccatttgact ggatgctctc tgtggcgaaa agagaaacca
     7801 gactggcaga tgatgtaagg atcttgcgcc aggaggaacg ggaaaggcgg agaaatatag
     7861 aaaagctaat tcagattgag cgtaacgtga aagcgcggat acgcagggtg acaaaatcca
     7921 gaactgaata attaaattta gctctgttaa aaatttaatc cttaaccgga ggtatatcaa
     7981 tgtcaaacgc acagaaagtt attaacgctg aaaaatataa cgagtgggtg aaaaagttct
     8041 cagagcagat ttttaaaatt actggcgacg agaatgcggc aaaaaatgaa ttagagccgt
     8101 ggacacctga aggtgtcgac ccaaattatt gctggtggga tgttgatcca gttgatgctg
     8161 caaatgaagc tatgagttat cacaacgatt aatgtcagga ggccgcccga aagggcggta
     8221 atgaaaagtg actgaattaa ccaaagagaa attaatcgaa gaagccaaat taaaaatagc
     8281 gattgcgaaa tgctacccca attcagggat ggcacgggta gagggcgagt tattc
//
LOCUS       DAZFBR010000166         8212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFBR010000166 DAZFBR010000000
VERSION     DAZFBR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.166"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(1..339)
                     /locus_tag="ACHJZ2_004473"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="ACHJZ2_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(655..1299)
                     /locus_tag="ACHJZ2_004474"
     CDS             complement(655..1299)
                     /locus_tag="ACHJZ2_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570454.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1320..1589)
                     /locus_tag="ACHJZ2_004475"
     CDS             complement(1320..1589)
                     /locus_tag="ACHJZ2_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4570455.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1668..2306)
                     /locus_tag="ACHJZ2_004476"
     CDS             complement(1668..2306)
                     /locus_tag="ACHJZ2_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO4570456.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2291..3523)
                     /locus_tag="ACHJZ2_004477"
     CDS             complement(2291..3523)
                     /locus_tag="ACHJZ2_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4570457.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDQMYRADVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRPLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            complement(4024..6192)
                     /locus_tag="ACHJZ2_004478"
     CDS             complement(4024..6192)
                     /locus_tag="ACHJZ2_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO4570458.1"
                     /translation="MIFPFGLNESQLLAVERAFSSQISVIEGPPGTGKTQTILNIVAN
                     ILIQNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVL
                     PDSPSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFS
                     HKYRFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSL
                     QLSYYEKNIREKQISLNEYEEAFKESDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFS
                     AETYRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCA
                     RNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHY
                     RCHPKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHY
                     WDEESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQH
                     SRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFE
                     SPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHS
                     QIALNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQA
                     ERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(6189..6704)
                     /locus_tag="ACHJZ2_004479"
     CDS             complement(6189..6704)
                     /locus_tag="ACHJZ2_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570459.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKNVIRHGV"
     gene            complement(6945..7991)
                     /locus_tag="ACHJZ2_004480"
     CDS             complement(6945..7991)
                     /locus_tag="ACHJZ2_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO4570460.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
BASE COUNT         2209 a         2099 c         1659 g         2245 t
ORIGIN      
        1 acctgattct ggaaaaggac tttgtggatt gttgctattg ccagtgagat tgaatgaagt
       61 cgttttaact cgctttgaat acgtcttgcc cccagatttc gctgagaacg taactccaga
      121 atcagagcga cttcgcaagt gccggttttc gttgatggtg aacgcttggg gcgacggctg
      181 tggctttcca gaccagcgat gccctgagcc agatatctgc gccaccattt tacgcaatgt
      241 gggccgagaa atgccacagc gacggcatac aaatcccgca tcaccggacg tttcatagag
      301 ttttacccac tgtagccgct gctgaatttc cctgttcata atttggcatt agttcagctc
      361 aatcccgact taaaaagtcc ctacaggatg ttccggacct gaggcacagt tcatatcctt
      421 atccataaaa aatttagcta tgtctatagc attaatcttg acaagacgac gttagggcct
      481 ggcgatgaat catttcccct gccagatatc ctttggggtg caccagaact accgttttat
      541 atctatataa ctgaaacgtc cctcgccatc cttcatattt tgatgtagta acaccattca
      601 ctttcatcta ccccccgttt tattaacata tagtaaaaat cacctcgccg cattttattc
      661 ttttgaagta aaatcattta ccagttcact taaagctctc ttttgttcat catcatacag
      721 agattgaatc cggaatttaa tctcttctgc aaccagtctt gcatcgggct cataaaatat
      781 ttttgttatt cgctgtttct gatgttcagg ctggtcttcc atatttcgta aaaccagatg
      841 aaataatata tcataaagtg cgcaatgcca tgaataacca tcattggtta aaataagctc
      901 ccaaacccgc tcctgctcgg caataagctg cagaacaaag gactcaacct gttctgtttg
      961 ttcgggggca acacggtata gcggagcaat cacagcaaaa gatagttcat ttaaccagga
     1021 ttccgcttcc ttctcgctcc tgaacggtgg acagttttgt attcttcgtt cgtattcacg
     1081 ggtaatctgc tccagaatat cctccggcgg tgtcagccac ttattcctga gcaatacact
     1141 ggttgatgga tgacagctgg ctatttcagc aataatttca cgtaatgtct ccgggctgaa
     1201 cttcttcagc tgattgtagt gctcccgggt aaaaccatgt tcatcgcata aaagttgcca
     1261 tgtcattgat atactgtttg ccattccttg cttcatcatt aatacctgtt acctgaatat
     1321 caacagaccg gtgtaccttt tctgaatacc tccccggacg gagtggcaat ccccattcgc
     1381 ctcagcgtgg catctgcagc tttcgccacc tcgctgatat ccagcgtttc aggtagcggc
     1441 ggggacgata tctgcgccag acaataattc cggcgcaaca acaaccactc ccgttgtgct
     1501 tctgtacatt caacatttcc cataacaaaa agccagcggt cagaagctct gcgccagcaa
     1561 ccagcagcct ccagtttctg cgcaaccatg tcttccacaa cttccggggt actctttttc
     1621 ctgctcaaaa cattaactca ctgaaataat atacatatct gacggtctca gcgaaccgtc
     1681 catgccgggc tgaaatcctc ttcctgaaac agctccgtca gcccgctaat ctgtttcaat
     1741 ctgagaactt catcctggtc catacccagc tccgtaccaa tgcgttcatt cgtccagccc
     1801 aggcgcgaca ggtccctgac aatatcagac attgaggtta tctgatgttt gcccctggcc
     1861 cggttatgtc ggatggtcgc agcaatacgt gaggcctgtc cctttctctc cggattaata
     1921 cagaccaccg gcagccagcc cttcagacgt tttcccgtac ctgcttccct gcccagcacc
     1981 tggcgatgaa aaccatccac caccaggtaa tggttctttt cctcagacac caccaccggt
     2041 tgtgtgaaac catctttctc cagtgactgc ttcagcagtt tcttctcacc cggcgccatg
     2101 acattaggat tgtaatcatt ggccaccacc tcatctgctt ttacccacag cacacagtcc
     2161 actggctcat gtgcaaaagg actgacttca tgaagcactc tgcgcaggtg attaagggcc
     2221 tgtatggtct cttcttctgg tcgttcctgg ctgaagtact gacgaatcat gttgccaatt
     2281 atctccggtg tcacagaatc ccccattcct tcctcctttc tttcatacgc tgcagataac
     2341 gttcatagtg ccggggtttg ttcggactga aggagagggg ccggcaccag aaatcattct
     2401 ttataagtgt cttacagata cgccgccagg acggaatatc ccggctcccc aggtcattct
     2461 cctgttcatc cgggatgtca tccgggaagc cccgcgtctg ataccagcgc aggtagacag
     2521 caattttatt acggtaatgt tctgccgttc tttccggcat cacatccagc aggaacatcg
     2581 catagctgcg ccaggtatga tgggctggtt ttgatatacg tttacgcagg gcaaaatagg
     2641 caccgctttc attggcataa agcgccccgc tggcagcgcc tgacacccgc tcacacatcc
     2701 tggcccaggt ttccggctcc agaacatggt aaagccacag ccccttacgt tgttcaggac
     2761 caaaaggttc acagacacgc atgttgcgta acggcacgtc ggcacggtac atctggtcat
     2821 acagaggatt gtaaatggca caagctctgg cgttatatat ccagatatcg cgggctttcc
     2881 agtcatacaa cggatacatg gtgtaataaa acccttcagg cgatgccgtg gtccagggct
     2941 tatcatcggc atatctcagt ttgcgctgag acaccagccc cataaagcga ttgagggatt
     3001 catccgcacg aacaccggtc agcactgcca ctccacaccg gttaccggca aaccaggaag
     3061 aaaatgccgg aacaaattct tcaaacgtca tggcataccg gaaaaaggga aaatatgtca
     3121 tatccgtaat ggcctcttcc ggtggctggc gaacccaggt cactcctggc tcccagcata
     3181 tccactccgg ctgaaactgc gagacgccgt ttaccgtggt caggggaagt gccacccagt
     3241 aaaaggtttc cgtcacatca tggtacattt cccgcatctt ctgaatatgt tcaatggtgc
     3301 accgatactg ggcctcccag tcaatgaaca gaacagagaa atgacgtttt ctcctgcggg
     3361 ccacctctgc cacaagatgg aaaagcacgg tggaatcctt tccacctgaa aaagacagac
     3421 ataccgacgg aaatgtttca aaaatccact caatgcgggc gattgcagca cgcaaaacat
     3481 tttctccggt aaggattttt tcggttaaaa gcgtatcact cattttcctg gctttatgtc
     3541 gaataattct gtttatacat tcaggcaaac ttcattaata actcaccgct ttggggataa
     3601 ataataacaa caaaaatctt cagatattaa cctggttgtt ggatttgtta tcacatcctt
     3661 tatccacctg cttcttactg gtaacacacc tggaaaatcc atgcattttc aacatatact
     3721 atcctttcaa cttgtaaata agaaaatatg atgcagatgg tgaacaattc aaatatcatt
     3781 ttgcaggaag tattcactgt aatagaatta ccccctgata aagtttattc actaaggaat
     3841 tattcaacag aaacattccc tgttagcttt gagcaatagc acttattttt atatgtatca
     3901 ggaatacgta cgttgatgta accaaaacaa agaagcctgt gtccatgggg ttcgtacaat
     3961 tgtgtgtggc acatgaccaa atcaggtttg agacctcccg ctgcaatatt ttttattcag
     4021 attttagcct gtcaggcctg acagaaacgc tcccaacttc ccttctatat cactgtctat
     4081 agtacgtaat cttagcaatg gcaggccgca tttcttcagt atactgtttt taagttcatc
     4141 acgttcagcc tgaacactgg tcagatgata acctccatcc acttcaataa cccctaatgg
     4201 agtcttcccg accttatagt aaacaacaaa gtcacaactg gcccggttac gcatgaataa
     4261 ctgttcacgg tgtgtaaaat ccccccgctc cagcaacacc aactgattca gcgctatttg
     4321 tgaatgaaac atcatactcc gataactatc ctgcgacagg atatcccgca acaggcaggc
     4381 cacaatctgt tctgacttaa agtgtgaatc atttgaattc aatctactat tcagtcgttc
     4441 cagtgatttg tcatactcac tgtacagcaa atcaaatgct gatatgaccg ggctctcgaa
     4501 aatctctccg tcatcagcgt agtatttgat gtaacgaatc agcgccgcaa tatgtccagc
     4561 gtggcgttca aatacatcat taccagtcac cagcgtaaat ttatttcggg cccttgaaac
     4621 agcgacattc accagttcag gattatcaac aaacgcgatg ttccgactgt gctggctgct
     4681 gcgcttctta tccagcacag tggaaaacac aatttcatcg cactcccgtc cctgaaattt
     4741 atgcaccgtc gatttcacaa aatcagcagg cagcactttt tcagccagat taacctgtgc
     4801 attatatggg gcaatgtatc ccctgctact ttcttcatcc cagtaatgac cttccagaga
     4861 ttcaagctcc ctgagatttg aaaaattacg ggtatggttt ccttttgccg tgatgaccag
     4921 agataatgag gcttcaccgg aatcaaccgt taacggtatc agagcattat cataaaactg
     4981 cttattacaa aattgaatga ttttaggatg acaacgataa tgctctttaa gaagggttac
     5041 tggcaccatg ttcctgaaca acatacaaac cgaatcaagt agactatatt tctcacaatt
     5101 atagtattct gccggtggag atgggctgtt cgggagtaat acaggaacat gtggaagttg
     5161 cttacgatcc cctacaacga ttacattacg ggcacatccc agtcccagaa tgcccggaac
     5221 aatatcctgc tgcgaggctt catcaataat aacataatca agaagggccc ccttgccgat
     5281 agagttaata atcgaatgcg tactgctacc aataatcgga aaacgcttta taaaacggtc
     5341 aaattcatcc ctgtatgtct ctgccgagaa tgaacttctt gttgaaacat ttcttcgcag
     5401 atgttgcttt aaataaagca tagaccatga tgtcaggcgc cccagtaaaa ttttaaaatc
     5461 agattcttta aatgcttcct catattcatt cagagaaatt tgtttttccc ggatattttt
     5521 ttcataataa gaaagctgaa gagaagtaaa tagcgccagc cttctctccg gaatcatcaa
     5581 tggtttgact ttcagtatca tgaagtttaa caacaaatca attctgtttc tgaatccaat
     5641 ccgcctgtca gaaaggtaat gaatataagc catcaggtcg gttgtttttt gtgaggaaaa
     5701 tcggtatttg tgagaaaaaa gctcctccgg tcgaagattc tgactctgcc attgctgcag
     5761 gtatttatac tcaatattca gctcattaat ttctgctttt aacgaagcga cctggttaat
     5821 ggcattcagg tgttttttca cttgttgaag tacatcatca atcgcatttg cagacggtga
     5881 gtcaggtaag acttcctcag aacgcgaaat cgacgttgaa aaaaactgtt gcctgttctc
     5941 cgtactcccc agtcgtgcca tcacatatcc cagttgctgt ttgtccatct tttcataaac
     6001 attactgaca gctgagttat tattggacaa aattgccact gttttattct gaattaatat
     6061 attagcaaca atattgagta tagtctgtgt tttacctgtt ccaggtggtc cttcaataac
     6121 tgaaatctgt gaggaaaatg cccgttcgac agccagcaac tgactttcat taagaccaaa
     6181 cggaaaaatc aaaccccatg gcgaatcacg tttttactct gtcctgaaca gtatgcatgc
     6241 aaggcggtat ccggatgagg aactattttc tttatctgcc taaggatatt ctcatcgata
     6301 tttctgttat taccacgggc ataaaaaata cgttcattag ccaccctaca caagtaatta
     6361 aacacggcat gctctgtaaa tgctgaacac tgttgaaagt caaggccggt cgttttcatg
     6421 atgtagggtc gttcaccctc gcggtaataa acagcagcat atttatcacc atatataata
     6481 accagttcag cccgggatgt tacagcgttc cctttccttt cgaggaaaac atttcgcaaa
     6541 tttacagttg gcgttatctg acatacagaa agcggacgag tatattttct cccgctgcga
     6601 aaatgacagg tcagcataac ctctctgtcg tcatttccag cccacagcgt ccagtcggta
     6661 attttatgag tcttattttc accgtcaaca caaatcgtga ccatattctt actcagaaat
     6721 tatcatttaa cttacggaaa attgttaagg cactcccgat attagctccg gaggattttc
     6781 cacaactccg ggactgttaa catatttctg cctgccatat atacattgaa tttagtcata
     6841 acagtcaata ccagaattac aggaatgcga tgccgatacg ctggttattg aatactggta
     6901 gcccctgagc actaattcat cacgatactc tgtatttact gttttcactg ctttttacaa
     6961 ccattccccc aggtaaaatg ctgctcctcc ggtgatggct gtgtcagaaa ctgccatccc
     7021 ggaatagcca cgctgaccgc cagcaatgcc gcacagacac cagccacaaa cggtttcaga
     7081 aacgtgggcg gggatttctg aagggagggt gactgctccg cctgcacttt ccccttgcga
     7141 ttatcaaggc tcagtgcatc cagacgcacc gcgccatttg ccgcaagcag cccttcccgg
     7201 ttatccacgc ttcccggcag actcagcgaa aaatcccctg ttcccgtctg aatcagactg
     7261 cctttacgat ttgacaggct ttgtgccgat atctgcagtg tgtcggcatt tatcgtaccg
     7321 ccattactgc tgaactgccc ggcagtccgg gcggaaagcg tatcggcact cagttgcgca
     7381 ttctgtgtag acagatttcc actggttgca ttcagctcag tatgttgtcc ctgtatccgg
     7441 ctgttaccta aatccagccc ctgctcaatg tcccgtaaat ccagcagaga accttcgtgt
     7501 gccgggaatg aggaatacaa ctgaatatag tcatcactgt ccggtgtcag acgcacatgt
     7561 cgcacaacac cgggaataat aaccagccgg aggataccgc tttttaaatc ctgcgaaggt
     7621 gccagaacac gggtggtgac ataaccatga tcgaccagac ggttctgcaa tgtactcatc
     7681 agcagattaa ttcctttcgc ccccaggcaa tgcccgaccg ccccgttggc gattttttgt
     7741 aaaggcagcc agtgtggtaa cgcatcagcc cccttcagtt ccgtctgttt aatctgaaaa
     7801 cagggcgttt caacaggaaa attaatctta tgggcaaaag agccaggtgc agaaaggcga
     7861 acatcagggg ctgaaggcgt cagttgttgc tcaagggctt cctgacgctg ctgctgaatg
     7921 ataaatgctt catctccgct acgccggaca tccgctgcct gaagagataa aggcgataat
     7981 aaagcagaca tcagtgtcag tgacagcaag gacggacagc gccggaataa gctttgttcc
     8041 tcagtcctct cgttcctgca acataatttt ccagtcaaac gaaaaacctt ttattcatgt
     8101 cataccaaca atcaccatga accgccccgg gaatcctgga gactaaactc cctgagaaag
     8161 aggtaaacag gatgactaaa aatacacgtt tttcccccga ggtccgtcaa cg
//
LOCUS       DAZFBR010000167         8136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFBR010000167 DAZFBR010000000
VERSION     DAZFBR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.167"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(68..259)
                     /locus_tag="ACHJZ2_004481"
     CDS             complement(68..259)
                     /locus_tag="ACHJZ2_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4570461.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(256..444)
                     /gene="dicB"
                     /locus_tag="ACHJZ2_004482"
     CDS             complement(256..444)
                     /gene="dicB"
                     /locus_tag="ACHJZ2_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4570462.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            1018..1203
                     /locus_tag="ACHJZ2_004483"
     CDS             1018..1203
                     /locus_tag="ACHJZ2_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570463.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(1390..1779)
                     /locus_tag="ACHJZ2_004484"
     CDS             complement(1390..1779)
                     /locus_tag="ACHJZ2_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570464.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(1791..1919)
                     /gene="ydfB"
                     /locus_tag="ACHJZ2_004485"
     CDS             complement(1791..1919)
                     /gene="ydfB"
                     /locus_tag="ACHJZ2_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO4570465.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(1921..2076)
                     /gene="ydfA"
                     /locus_tag="ACHJZ2_004486"
     CDS             complement(1921..2076)
                     /gene="ydfA"
                     /locus_tag="ACHJZ2_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4570466.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2354..2641)
                     /locus_tag="ACHJZ2_004487"
     CDS             complement(2354..2641)
                     /locus_tag="ACHJZ2_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4570467.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKETPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(2641..2832)
                     /locus_tag="ACHJZ2_004488"
     CDS             complement(2641..2832)
                     /locus_tag="ACHJZ2_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO4570468.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(2860..3261)
                     /locus_tag="ACHJZ2_004489"
     CDS             complement(2860..3261)
                     /locus_tag="ACHJZ2_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4570469.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            3370..3642
                     /locus_tag="ACHJZ2_004490"
     CDS             3370..3642
                     /locus_tag="ACHJZ2_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4570470.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            3626..4051
                     /locus_tag="ACHJZ2_004491"
     CDS             3626..4051
                     /locus_tag="ACHJZ2_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4570471.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(4258..4713)
                     /locus_tag="ACHJZ2_004492"
     CDS             complement(4258..4713)
                     /locus_tag="ACHJZ2_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570472.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            4792..5901
                     /locus_tag="ACHJZ2_004493"
     CDS             4792..5901
                     /locus_tag="ACHJZ2_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4570473.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQ
                     QVMTAGSEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRA
                     GANPKKSAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDR
                     NFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            5908..6654
                     /locus_tag="ACHJZ2_004494"
     CDS             5908..6654
                     /locus_tag="ACHJZ2_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4570474.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            6676..7446
                     /locus_tag="ACHJZ2_004495"
     CDS             6676..7446
                     /locus_tag="ACHJZ2_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4570475.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            7462..7875
                     /locus_tag="ACHJZ2_004496"
     CDS             7462..7875
                     /locus_tag="ACHJZ2_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4570476.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT         2191 a         1733 c         2115 g         2097 t
ORIGIN      
        1 agggtgaaca aatccctgcc attgctggca tataaaaatg aaaccggata ttaattacgg
       61 tgctgtttta aagtcctgcc ggtatttcgt tattattagt gtgagtagtt ttatctaccg
      121 gataacagtt accgggaatt ttttgttctg ctgctgcagc catgcattct ttcattgaac
      181 cgtataagcc agtcaccagc tcaagagatt cgccggaaac aagataaact gtcagaacga
      241 gtgcaaatgt tgtattcatt gtttatatcc tttttgcagc aggtccagac gagccagcat
      301 tgaaggaatg catacttcat ttaacaggtc ctgctcgagt tttctctgct taatggcgtc
      361 ttcaataaat gttttgtctc cagtgataac gccaatttcg aaacgaagtt cagacgtgct
      421 ggcattacat gataactttt ccattatcgc gtcctcaaca atgaattttg tgatgcggtg
      481 cctggtgcct ccaggtgacg ttaaccagtt aacaattaac gccggataca gagcattctc
      541 gttactcagc aaatgaccgc tttaccgatt taactgttcc gcgtgcgctg agccgcattc
      601 accgcatcac aaaattcact tttaaaaaag ggcggcagag cagttacgga gtaaaactga
      661 taccgccaaa tgtcaccaga atattgataa cagagggcgt tgcagcgggg ttgtcactta
      721 agcgtatggt caacctgaca acccggtgtc ctcaacgggg aaagaataac cccgccatac
      781 ttaccgccgc gccatttcgc ggattgccac aaccggaagc gcacggtcga attaaattta
      841 acgacggctc taagggagaa ggacttcgcc gtgcactttc gtgttatgcc ctgacttttc
      901 agggatatat cctttcagta aactgtcagt gccggattct tatctgtgtc cggcgcacga
      961 ccacacgtag cagcgtgttg gtctccattt ttaattcagc ccttaatgga ggataaaatg
     1021 ccaaacaaac catgccctgc ctgtaacgct ctttctggct ttttagagaa gggcggttat
     1081 tacattttta actgtccaga acatgttgaa tttcatatat caaaacttga cagtatcatt
     1141 acaaagccaa atcagtatca gtctgattta ctgaataaag agttaaacgc cgcccgagat
     1201 taagggcgta gtggcccatt aaaaatattg caaagaccaa ctattgttga actggattaa
     1261 accagtaatt ctctattgct cttattgtta ccatcggatg attgccgggg gttattttaa
     1321 gttcgggagg aaaaggctct ccatcagcag ccatcattga gtatgtgaaa tccgccgacg
     1381 cagtcccttt catgataatt ccgaattttt caagtaatga gagcagttcc ggtaatcctg
     1441 tgtcatctga ataactttcc tcatagaaag aagcgattgc cgcaggagat gacagttctc
     1501 gctctgcaat ttttttgtag ttttccagaa ttcttttttt gaatactttt cctcgacatg
     1561 aaaacccatc accgaggcaa tccgctttgc agtttcttca gtattctggt tgtttgttac
     1621 cgaaacagag tacaatccgg taaacgcatc gcgcacatta cgagccatat tatcaatgta
     1681 tttcttcgtt gcagattcta attcaacttc gttcagacgt tggtgaagtg catgtgctgc
     1741 aatttcctgg atttctttta gtaaatcttt aaattccatc tttaactccg ttagtcgtta
     1801 gattttgccg aactggaaag cgcctgttta aactcactga agctgagagc ttcctcgcct
     1861 tcggcaaggc cttcgaagta ttcttcgtaa gccttttcca tgattgtgtc gaaatccata
     1921 tcactcacct gagtttcttt ccagccagcg acgggcacca ttttcggttt taaacgtttt
     1981 gcttttggta tacgtcatcg cggtgaatgt accgtcctgg ttgggaaaca cgccgtacac
     2041 cagagattcg ttgttgccaa gatcgatagt atccatgttg acctcatttc cccttaacgc
     2101 cgggtagcgg aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc
     2161 atacgcctcg ggctggctac ttaccccctg accactgctt ggtaactcga agtattgccc
     2221 ggcgttctgt ggggcggggt gggtggttgg tgtatgtaat ctacaataaa aaactgtttt
     2281 tgtgtcaaca gtttttaatt gttgttttgg gcaaaaaaat ccctcgaaag agggagtatg
     2341 aaaattgttc aactcagata gagaagggaa attgtcgccg agaatgtgtc acgcttacaa
     2401 tctcaatgct tgaagcagct actctgtaca ggattatgta gttagggtgg gtcacgatct
     2461 ctctcaatcc agaaacccgt tcgcttggtg gatataagta cggatgctca gatagaggta
     2521 ataccgatgt ttcaatgcgt atttttagtc tacgtgctgc cggtggggtc tccttggcga
     2581 tgtaagttat gatctggcgc aaatcatcgc gagcagacgg tagccataaa atgggtaaca
     2641 ttactcgctc ttgttagtta cagcaatttg agcaataaga ttttccattt cagccattac
     2701 ctcgtcatgt ggaattgcgg ggcgagtgtc tgcaaggctt gacgttactt tagtgcgcaa
     2761 ccattcgttg taactgtttt cttgttcggt agtttcgaat tctgaaacta tcggagaaag
     2821 ggctgtaccc atggcataac tcctcttctt gtactgtggt cacgcccggc ggcttttttg
     2881 tgccgccagc cacctagcaa tggtttcttc cattgatttt ttcttgtctt tgatttcttg
     2941 aagcattttt tcttggtctt cctcaggaaa cgcactaaaa gcttggagca gttcgcgttg
     3001 gcgaggacca attttcatcg tgtcaggggt gaaaatttgc tcacactctt caggaggcaa
     3061 taaaaaccaa tgcaatggat gccctgaaac ctcaaccagt ttatccaaac ttgaggcttt
     3121 aggtgtagcc ttaccgctga cccattgttg aacagtttgt tgtgtcacac caattctacg
     3181 ggcaagctca gcctggctcc atccagtttc ctgaagaagc ttgctgattc tgtacataga
     3241 tacttctagg gcgctcatca ttattcaatt ttacaggtaa atactgttaa aagcatcaca
     3301 ataaaaaact gtttattgca tacagttttt tattgtaggt tttgcttata gtttttagag
     3361 gagggcaaaa tgctagatag cactcgcgaa aaaattaggc agaaatacac tcaggctgaa
     3421 ataggtcgtt atatgggggt cgctcaacag actgtttggc aatggtttag ctttggcgtt
     3481 cccccaaagc aggtaattcc gttatgccaa ctaatgaagt gggaagttac cccgcatgaa
     3541 atccgcccag atatttatcc taacccaacc gacggtttac ctgttggatg taaggttaac
     3601 acatcaaatg cgccggagtt gattcatgaa aatcaagcat gaacacatcc gcatggcgat
     3661 gaatgcctgg gcgcatccgg acggcgaaaa agtaccggct gcgaaaatta ccaaagcgta
     3721 tttcgagctg ggaatgacgt tcccggaact gtatgacgac agccatccgg aagccatggc
     3781 tcgcaatact cagaaaattt tccgctgggt ggagaaagac actcctgatg cggttaaaaa
     3841 aattcaggcg ttgttaccag cgatcgaaaa agcgatgccg cctccgctgg tggcccgaat
     3901 gcgcagccac agttccgctt attttcggga gttggtagag acgaaggaac ggctggtgaa
     3961 agatattgat gatttcgttg catcagcgat cgttctgttc gatcagatga atcgtggtgg
     4021 cccggcagga aacactctgg ctgtgcatta attgggtaat aaatatgagt aatgacaaaa
     4081 aattgacact gagcgtttac gaaaacagtc cgcacatctg gcgtggcggt ttatctgatg
     4141 tggagctggc agagtggttg atacataaag ctaatgcgct gctctggcgt ttgtcagcca
     4201 gagaacagcg caaggaaacc agaataaagc tggctgatgc agaagcgtgt gccgggctta
     4261 ttgaggatta tacaaatctt ggtatttctt cagcagagag tgatcccatt cagcctctga
     4321 gcagggagtc aatccagcac gctggttgta tggcacatct tgtaactgct cgtcaacatg
     4381 aggtgggtat tggatcactt ccggcgggat attcgctgat tccagagctg gttgaagcaa
     4441 gaaaatcagt tcagaaaaag agagatgacg cacttcaatt attgagagag cactatggcg
     4501 cgataccaga atgcgaacag cgtcgatacc ctgaaggtta tgaatggatg cagtctcttt
     4561 ttgaagttcg ctaatcaata tgtcgagacg aaggtatgtt tcggcgcgca gccaggctct
     4621 gtaatccggg agcatttcgg ggctgttaca ccagcggttt gttgctgcaa catttaatac
     4681 atgagcctga taaaggcttt tcaaaaaata catgtcgaac ctcctctggt tctgtcgatt
     4741 gggaaccaca gattatatcc ggaggaaggt tcggcaccag atgaggtagc catgcgtgat
     4801 tacgcaaaag tttctccgcg attctggctg ggagaaacgg ggagagaact tagaaaggcg
     4861 ggtgcagaag cgcaagttgt tgctttttac ctgatgacat cccctcacgc aaatatgctg
     4921 ggtttgtatt acctgccagt tttatacctt gctcatgaaa ccgggcttgg tctggaaggg
     4981 gcttcaaagg ggcttaaaag ggctgttgaa gctggttttt gtagctatga ccatgatgca
     5041 gagatggtct gggtccatga aatggcagcc tggcaggttg gggaaacgtt gaagcctggc
     5101 gataaccgtt gtgcaggtgt caggaatgag tatgcatcat tacctgaaaa cgcttttctg
     5161 tcagtgtttt acgacagata taaaacggat ttccatctgg atgtgaggcg gaataatagc
     5221 cgaaattcgg taaggggctt cgaaggggct tttaaggggc ttcgaagcca agaacaggaa
     5281 caggagcagg agaaagaaca ggaacaggag aaagaacagg aacaggacaa aaacactatg
     5341 gttcatggca aaaaaaacac cacgaaccag gcaggggatg ttcagaccgt caatcctggt
     5401 cagccagcag gcacgacacc ggaagccgat tcgggcgctg tgcagcaggt gatgaccgca
     5461 gggtcggagc aatcacacca actgcagcag cctgaagccg attccgccat tcagcgggaa
     5521 gccgatcggg tagtcccgga aagcaccggg cagtctgtgg gacgagtgga ttatccggat
     5581 gtgttcgaac aggtctggcg ggaatacccg ttgcgtgctg gggcaaaccc gaagaaatcc
     5641 gctttcagtg cctggaaggc cagattgcgc gagggggtgc caccagagac catgctggat
     5701 ggtgtgaggc gttacgcgag atacctggcg gcgaccggga aagcgggaac ggaatttgtt
     5761 cagcgagcga cgacgttttt tgggccggac cggaattttg aaaacccctg gttgctcccg
     5821 gtaagcggca cgaacaacca gcgttgtgtg aatcatattt ctgaaccgga taccgaaatt
     5881 ccgccgggat tcagggggtg atgtggcatg aaaaacattg cggcagccgg ggttcttgaa
     5941 cgtattcgca gacttgcacc acaggcgtcg gttccaccgt accggacggt ggaggagtgg
     6001 cgggaatggc aacttgctga aggacgaaaa cgcagcgagg agattaaccg ccagaatcac
     6061 cagttgcggg tggaaaaaat cctgaatcgt tcgggcatcc agcctctgca cagcaaatgc
     6121 tcgtttgcga attatcaggt gcagaacgac gggcaaaaat acgcgctgag ccaggccaaa
     6181 tccatagctg acgaactgat gaccgggtgc acgaattttg tgttcagcgg taaaaccggc
     6241 accgggaaaa atcaccttgc agcggcgatg ggtaaccggc tgatggcgaa ggggcgcagc
     6301 gtgattatcg tcaccgtgtc tgatgtcatg agcgtgttgc atgacagcta cgacaacggc
     6361 aaatccgggg aaaaattttt acaggagctt tgcagtgttg atttgctggt cctggatgaa
     6421 ataggcgttc agcgggagac gaaaaacgag caggtggtat tacaccagat aattgatcgc
     6481 cggacagcat cactgtgcag tgtcgggatg ttaacaaacc tgaatcatgc cgcaatgagt
     6541 acacttcttg gtgagaggat tatggaccgc atgaccatga acggtggtcg atgggtgacg
     6601 tttaactggg atagctggcg tccaaatgtc agcaatatga gggttgtgaa gtaattttgt
     6661 ccggaggaaa ttttaatgga aaccgtatct gacgcactga aagcactgaa aaaagcctct
     6721 tcacatgtgg tggcagctcg ccttggaatc agtcgtgaag aggctgtcaa cgagctgtgg
     6781 gaactcaaaa taaatggcgt cgttgataaa actggtcaca cctggtttct ggctggcgaa
     6841 ggtgaatccc gggtaaccga agagcggcca gtaaaatctg aagcacagga tatgctgacc
     6901 ggggaggtcg aacaaaaagt taccgcagac atgatgattg agtttatcgg tcaggatggg
     6961 gctaaaacgt gtgaggaact ggcgggtaag ttcggtgtca gtactcgcaa ggttgcttcc
     7021 acgctggcgg tggtaaccgc aacggggcgg ctggcacgcg ttaatcagaa cggtaaattt
     7081 cgttactgca tgccgggcga taatttacca gcagagccga aagccgcgct ggtaacggaa
     7141 agtgatggta aggcctttcc tcagccagca ggtgctgcgt taccagtccg ggaagccgca
     7201 acacaggaag aaattaaaac agaaactgtg gcggacattg tgcagccgtt gccatcgttt
     7261 accgaaacgc aagcagatga gctgattttt ccgtcccttc gcagggcaaa cctggcgctg
     7321 cgcagggcga aaagtgatgt tcagaagtgg gagcgagtct gcgccgcgct gcgggagctg
     7381 aacaagcacc gggatattgt tcgacagatt actgattctt cccgccgtgt tgtatcggaa
     7441 aagtgattgc cggaggcgct tatggcaaaa gtatttacac aggaagagcg ggaaaaaatt
     7501 aaagggcagg ttgttgagct agtacgccgg agtgggcgcg agacgttacg gcaactggaa
     7561 gccaagacag gtgcgacaag atatctgatg agcgttcttg ccagagagct ggttgccagt
     7621 ggcgatgtat acaactctgg ttacgggtta ttcccatctg aacaggctcg taaggactgg
     7681 caaaacgccc gcaaaaaact atcgagggca aaactgaaga aaccggttgt ggttgatcct
     7741 gaccttatct ggtcattacc agacggagaa atacgccgct atgacaggca attgaacata
     7801 atttgtagtg agtgccgtaa cagtgaagtg atgcagcgag ttttgatatt ttacacagga
     7861 gtaatgatgg aatagtgaaa ataacatgaa acctttggat tctggtgttt cagtggatgg
     7921 gaaaagagca gatacataga aaatgaatag caataattca cagtctggat gttgtttgta
     7981 tgcttataaa atgatcctag tatgcattgc cggagttgaa tcagctccaa tgaagaaact
     8041 gagaggagta tttagaagat agctaaatgg atgtaattat tagtatctaa aaaatagtat
     8101 ttttgaaatg ggtctaagaa cacaacaatg tacaaa
//
LOCUS       DAZFBR010000168         8106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFBR010000168 DAZFBR010000000
VERSION     DAZFBR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.168"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            118..594
                     /locus_tag="ACHJZ2_004497"
     CDS             118..594
                     /locus_tag="ACHJZ2_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO4570477.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            591..2714
                     /locus_tag="ACHJZ2_004498"
     CDS             591..2714
                     /locus_tag="ACHJZ2_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4570478.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLWTERAEDNPWITSGGWL"
     gene            2711..2923
                     /locus_tag="ACHJZ2_004499"
     CDS             2711..2923
                     /locus_tag="ACHJZ2_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570479.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            2923..4425
                     /locus_tag="ACHJZ2_004500"
     CDS             2923..4425
                     /locus_tag="ACHJZ2_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4570480.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGSKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            4370..6394
                     /locus_tag="ACHJZ2_004501"
     CDS             4370..6394
                     /locus_tag="ACHJZ2_004501"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO4570481.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            6482..6808
                     /locus_tag="ACHJZ2_004502"
     CDS             6482..6808
                     /locus_tag="ACHJZ2_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO4570482.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            6801..7082
                     /locus_tag="ACHJZ2_004503"
     CDS             6801..7082
                     /locus_tag="ACHJZ2_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO4570483.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            7085..7708
                     /locus_tag="ACHJZ2_004504"
     CDS             7085..7708
                     /locus_tag="ACHJZ2_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4570484.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            7721..>8106
                     /gene="gpU"
                     /locus_tag="ACHJZ2_004505"
     CDS             7721..>8106
                     /gene="gpU"
                     /locus_tag="ACHJZ2_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO4570485.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
BASE COUNT         1881 a         1927 c         2540 g         1758 t
ORIGIN      
        1 ttagtattaa aaagatgatg attgtggttg ttttttgttc gacatcttta tatggcggca
       61 tttctttaca aaaaaacaga gtcacttctg ttctgcggtt tatgtggagg gatgtaaatg
      121 gacggcgagc tgaaaaatat gaagttaaat attaatcaac tggcagccct ttcaggtctg
      181 caccggcaga ctgttgccgc cagaatggcg gatgttcctc ttgcaccagg cagtaatgaa
      241 aagaaaaaac tgtatctcct gacggatttg ataacttcgt tgctggaaaa accaccatct
      301 tccgaagatg aggatatgga tcctcacgct cgtaaggcat ggtatcagtc cgagcgcgag
      361 cgtcttaaat ttcagcatga aactgttcag cttgtgccag tcagtgatgt caggcggtcc
      421 ttttctgtcg tggtgaaagc gatagttcag gtactggaaa cctggcctga ccggctggag
      481 agggacaggg ggtggaccgc atcacaactg aatgaagtac agattgtggt tgatgagatc
      541 cgcgacacac tggaaaaggc agtcattgac tgttgtgatg aggccgatat gtgaatcagg
      601 tgaacgagag ccatagccgc gcatccgata tctggcgcga agtggcctcg ctgtttcgcc
      661 cacccagccg gttaccagta gcggaagcca tcaggcgtta tatgcgggtt ccacggggag
      721 ccaatacttc cggtccgtgg gagtcatcgc tgacgcccta tatgattgac cccattaata
      781 cattatcagc ccgtgaatat gacgcggtgg tgtttgtggg acctgcgcga accgggaaaa
      841 ccgaagggct gattgatggc tggattgtgt acggcatcat ctgtgatccg gcggatatgc
      901 tggtggtgca gatgactgag acgaaggcgc gtgagcattc cagaacgcgt ctttccagga
      961 cgtttcgcca cagtccggag gtcagcaagc gcctcagtcc ttcccgtaat gacaacaacg
     1021 tccacgataa aatgtttctt gacggctcct tcctgaagat tggctggccg tcgatcaccg
     1081 tcttttcctc ttcggattac cgtcgtgtgg cgctgacgga ttatgaccgt ttccctgaaa
     1141 acgtggacgg ggaaggggat gccttcacgc tggcctcaaa gcgtaccacc acctttatgt
     1201 cctcggggat gaccctggtc gagagttcac cggggcggga tatcaccgat accaaatggc
     1261 gttgtggtgg cgcacatgag gcaccgccaa caacggggat cctgtcactg tataaccggg
     1321 gagaccgccg ccggtggtac tggccgtgtc cgcactgcgg ggaatatttt cagccggtga
     1381 tggataacat gaccggatac cggaataacc cggattttgt ggctgccggt caggctgccc
     1441 gtctgatgtg tccgcattgt cgcgggctga ttgcccctga gcagaaacgc gaactgaata
     1501 accaggggat ctggcttcgt gaaggtgaac gggcggcggc ggacggcagt atcaccggaa
     1561 cgccacgaaa ctcccggatt gcgtcatttt ggatggaggg gccagctgcg gcgtttcaga
     1621 cctgggaaca actgattttt aaactgctgg cggcagaaga agagtatgag cgaaccggca
     1681 gtgaagagac cctgaaagcg gtggtgaaca ccgatatcgg acgaccctat ctgccccgtt
     1741 cagccacgga acagcgtaaa agtgaactgc ttgaacagcg tgccgagccg tttccccggc
     1801 gatctgtgcc ggatggtgtg cgttttattg aggcaacggt tgacgtacag ggcggtaaaa
     1861 atcgccgttt tgttgtgcag atcaccggat acggagagca gggggaacgc tggattgttg
     1921 atcgctacaa catccggcat tcactgcgct gcagtcccaa cggtgaaagt ctgccggttg
     1981 atccggcggc atatccggag gactgggatt tgttgctgac ggatgtgttc cataaaacat
     2041 ggccgctggc ttctgatccg gatgtgcgca tgcgtctgat ggccatggcg gtggatacgg
     2101 gaggggaagc cggggtgaca gataacgcct atcgtttctg gcgtcgttgc cggagtgacg
     2161 gactgggcaa cagggtgttt ctgttcaagg gggatggact tcgccgtgac aggctgatta
     2221 accgaacctt cccggataat accggcagaa gtgcccgccg tgccagagcc agtggcgatg
     2281 tcgcgctgtg gctggttcag acggatgcgt ttaaggatcg tgtaaataat gccctgtggc
     2341 gtgacacacc agggccgaac tatatccact ttcccgactg gctggggcgg tggttttacg
     2401 atgagctgac ctatgaagag cgcggcagtg acggaaaatg gcgaaaaccg ggcaggggcg
     2461 ctaacgaagc gtttgacctg ctggtttatg cggatgcgct tgccgttctg catggttacg
     2521 aaaagatccg ctggccctcc gcaccggact gggcacagcg ggaaacgtgg ctcgtcttcc
     2581 cgcaggagcg ttctggtgaa acggtatccc cggaactgac ggccggggca gaaaaacgcc
     2641 gtcgccggaa gaaaaaactg tggacggagc gtgcggaaga taatccatgg ataacatcag
     2701 gaggctggtt gtgagcacag aagaagccag agaaatgata cagcggtacc gtgaagcgga
     2761 aatggccgta ctggagggaa agtctgtcac cttcaacggg cagcaactga cgctggaaag
     2821 cctttctcag atccgcgccg gacgtcagga gtgggaacgc aggcttgccg cgatggtgag
     2881 ccgcaggcgg ggaaaaccgg gatttaaact ggcgaggttt taatggcaat tattgatgat
     2941 gtgatcggcg tgttttcccc cgggtggaaa gcagccagac tgcgttcaag ggcgttaatc
     3001 atggcctatg aggcggtgaa accgacccgg acacataaag cccggcggga aaatcgctct
     3061 gctgatcagc tcagtaaata cggtgcggtt tccctgcggg agcaggcccg ttttctggat
     3121 atcaatcatg acctggtgat tggtgtgttt gacaagctgg aagagcgggt gattggtgcc
     3181 aggggaatta ttgtggagcc tcagccatta cgaaaaaacg gggaaatggc ggctgagctg
     3241 gctgcggata tccgccgttt gtgggctgaa tggtccgtga gtccggatgt gacagggcag
     3301 tatacccgtc ctgtgcttga acgtttactg ctgcggacct ggctgcggga tggtgaagtg
     3361 tttgcgcaga tggtcagtgg tgcgggaaac ggtctggaac ggacggcggg agtgccattc
     3421 tggcttgagg cgatggagcc ggattttgtt cccatgcgca ctgatgaatc cgccggactg
     3481 aatcaggggg tttttcttga tgagtgggga agaccgaaaa aatatctggt ttataaaaat
     3541 tatccggtca gcggccggca gagtgatacg aaagaaatcg ctgccggaaa aatgatccac
     3601 ctgaagttca ctcgtcgtct gcatcagacg cgaggctcat ccatgttatc gggggtgctg
     3661 atgcggatca gtgcccttaa ggagtatgag gatgcggaac tgacagcggc gcgtattgct
     3721 gcggcgctgg gactgtatat ccgtaaaggt gacggacagg actatgaaga tccggggagc
     3781 aaagagaccg agcgggaagt ccatatcacc ccgggtatta tttatgacga tttgcgcaag
     3841 ggcgaggata tcggcatggt caaatctgac cgtcccaatc ccaaccttga aactttccgc
     3901 aacggccagt tgcgtgcagt ggcagcaggc agtcgtctga gtttttccag tgcggcgcgt
     3961 aactataacg gcacctacag cgcccagcgg caggagttgg tcgagtccac ggatggttac
     4021 ctgatcctgc aggactgttt tattggcgcg gtaacccgcc cggtgtaccg gacatggctg
     4081 aatatggtgg ttgcggcagg tctgctgaaa attccggcgg atgtggagat gaaaacgcta
     4141 tataacgcga cgtattccgg tccggtgatg ccgtggatcg acccggttaa ggaagctgaa
     4201 gcctggagaa ttcagatccg gggtggtgca gcgacagaat ctgactgggt gcgtgctggt
     4261 gggcgcaatc cggatgaggt caaacgtcgc cgcaaggctg aaattgatga aaacagcaga
     4321 ctggggctgg tctttgatac tgaccccgtc aacgacaaag gaggcaacag tgccggaact
     4381 gaacgacagt atcagcgcga caccgaaagc cagcatgaag aataaatcct ggttcaggat
     4441 gcaggctggg gggccgggtg acgcggatat ttatatttat gacgagattg gtttctgggg
     4501 agttaccgcg aagcagtttg tcagcgaact gaatgcactg ggtgatatca cccacattaa
     4561 tctccatatc aattcaccgg gtggcgatgt ctttgaaggc atcgccattt ttaatgccct
     4621 gaaaaatcag ggggcgacca ttaccgtgta tgtggatggc gttgccgcct cgatggcatc
     4681 tgtgattgcg atggccggtg atacggtcat tatgccggaa aatgccttca tgatgatcca
     4741 taagccatgg ggattcagtg gcggggatgc tgaggatatg cgcagttatg ccgatttgct
     4801 ggataaagtc gaatcggtac tgttgccagc ctatgcgcag aaaaccggaa aaaccaccga
     4861 tgaaattgcc gccatgctgg cggatgaaac ctggatgtcc ggtgccgaat gtctggcaca
     4921 cggatttgct gaccaggtga cacccgctgt tgaggcaatg gcatgtattc agtcaaaacg
     4981 tacagaggaa tttaaaaaga tgccggaatc catccgaaac atgattactc cgccacgcaa
     5041 cagtgccccg cgtgatacca cagtgacaat ccctgcaccg gcggtaacag aaccatcacc
     5101 ggtaccggca gtgtctgatg aggcgaccat tcgcgcccgc gttatggcag aacagaaagc
     5161 ccgcatgtca ggcattaacg atctgtttgc catgttcggt ggtcgctatc agacgcttca
     5221 ggcacagtgc gtggctgatc ctgactgttc gctggaaatg gcccgtgaac gactgctgaa
     5281 tgaaatgggc aaggagtcct cgccgaccaa caaaaataca ccggcccata tttatgccgg
     5341 aaacggcaat tttgtggggg acgggatccg ccaggcgatg ctggcccgtg ccggatttga
     5401 aaatgtcgag aaggataacg cctataacgg gatgaccctg cgtgaatggg ctcgcatgtc
     5461 actgacggag cgcggtattg gggtggccag ttataacccc atgcagatgg tcgggctggc
     5521 gctgacgcac agcacctctg attttggcaa tattctgctg gatgtgtcga acaaggggct
     5581 gatccagggc tgggaggaat cagaagaaac cttccagaag tggacccgta agggacgcct
     5641 gtcagacttc aaaacagcgt atcgcgtggg gatgggcggt tttggttctc tgcgccaggt
     5701 tcgtgagggg gcggagtata aatacatcac cacctcagat cgcaaggaga ccattgcact
     5761 ggccacttac ggggagattt tctccatcac ccgccaggcc attatcaatg atgatctgaa
     5821 tatgctggtg gacgtgccga tgaagatggg gcgtgcggcg aaggcaacga ttggtgacct
     5881 ggtctacaag gtgctgacgg ataacccgaa actgtccgac ggtaaggcgc tgttccatgc
     5941 cgatcacaaa aatattgcca ccggggggat ctccgtttcc ggactggatg cggcccgtca
     6001 gatgatgcgc ctgcagaaag aaggcgatcg tgccctgaat atccgtccgg cctttatgct
     6061 ggtaccggtg gcactggaga cggtggcgaa ccagaccatc aaatcggcca gtgtgaaagg
     6121 ggcggatgca aacgccggtg tcattaaccc tatccagaac tttgctgagg tgattgcaga
     6181 agcgcgtctt gatgcggcag acccgaaaac ctggtatctg gcggcggcac agggcactga
     6241 caccattgaa gtggcctggc tggatggtgt ggacacgcca tacattgatc agcaggaagg
     6301 tttcaccact gacggcattg ccacaaaaat ccgtattgat gccggagtgg caccacttga
     6361 ctggcgcggg ctggtgcgtt cgtcggtggc ctgataaccg cgttatcaca atcactgccc
     6421 gaaagggctt tttttatgcc tgaaaaacag ccccacaggg gctgtccgga gaaacagcat
     6481 tatggcgaaa aattttgtac aggacggtac caccattgaa ctggtgaatg ccggagatca
     6541 gaccatcctg agcggtgctg cggtggtggt cggcagtatg gtggccgtgg ccattaccga
     6601 tattcctgcc ggtgaggccg gtgacggttt tgccgaaggc gtgttcctgc tgcccaaaca
     6661 gtctgctgac gacattcagt ccggcgcggt ggtttatctg aaggacgggg ttgtgcagct
     6721 ggctgcagac ggtgcggtgg cagcgggggt agcctgggaa aatgcccctg caaacagcgc
     6781 cactgtggcg gtaaaaatca atgtctgatc tgtttacgcg aatgtgttgc cggatggacg
     6841 tggcgaccgt tcgggtgatg ggcaaacagg cggagattaa cggcgtcgtg tacgacgtga
     6901 tgccggagga agagtccgcg gagatggggg cgctttcggg cagccagttg tcactggtgg
     6961 tgttttcagc ccggtaccgt ccggcccgtc atgatgttgt tgtgtttgcg gggcgcacac
     7021 tgacggtgac ccgttatgac acgtacaacg gtaaaccccg gatttttgtc gaacaggaat
     7081 gagtatggca ataaaaggtc tggcgcaggc catgaaaaat ctggatgcaa ttgatcgccg
     7141 tgccgttccc cgggcctctg ccacgacact gaaccgcgtg gcgggggcca ttattgcgaa
     7201 aacggcctct tcagttgcca gggagctggc cgttccccgt cgtcttatcc gtgcccgcat
     7261 ccggttaagt ccggcacgac cggataaggt ttacgcaaag gtttacatca ataccggcaa
     7321 cctgcccgcc atcaaactgg gggaggcccg cgttcgactt tcccgcagaa aacggagaaa
     7381 gaaaggacag cgtgcggccc tgaaaggggg cggcagtgtg ctgattgtgg ggaaaagacg
     7441 gatcccggac gcctttatca cccggctggc taacggacgc tggcatgtga tgcagcgtat
     7501 gccgtgggca tcatcatcca ccggcgcgga cagcaaaggg aggccgaaac gccaccgtct
     7561 gccgatcgaa gtggtgaaga ttacgactgc cggaccgctg gcagaaacct ttgaacgtga
     7621 acgggaccgg atgtaccggg aaaaattacc ggcgcagatg atgaaagcca tgacgcatca
     7681 gttacgcctg gtgctgaaaa gaaaatgact gggagggtgt atgaaacacc gtgaaatacg
     7741 ggcggcagtt ctgtctgccc tgaaagaaaa tatttctgag agggtgagct ggtttgacgg
     7801 tcgcccggtt tttattgatg aacaggaact gcctgctgtt gctgtttacc tgacagatgc
     7861 gtctgctgct gacgagttcg ttgatgaggg aacctgggag gcgacactgc atattgaggt
     7921 ttttctcagg gcaaaagaac cggactcggc actggatatg tggatggaag aaaaaattct
     7981 tcctgcgctg gaggcagttc ccgggctcag tgcattactg ctgaagatga atcttcaggg
     8041 gtatgactac cgccgggatg atgagtttat gatgtgggga tcggcagatc tcctgtggaa
     8101 aattac
//
LOCUS       DAZFBR010000169         8087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFBR010000169 DAZFBR010000000
VERSION     DAZFBR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.169"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            128..1241
                     /gene="ompN"
                     /locus_tag="ACHJZ2_004506"
                     /pseudo
     CDS             128..1241
                     /gene="ompN"
                     /locus_tag="ACHJZ2_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            1382..1816
                     /gene="uspF"
                     /locus_tag="ACHJZ2_004507"
     CDS             1382..1816
                     /gene="uspF"
                     /locus_tag="ACHJZ2_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO4570486.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1992..2927
                     /gene="ttcA"
                     /locus_tag="ACHJZ2_004508"
     CDS             1992..2927
                     /gene="ttcA"
                     /locus_tag="ACHJZ2_004508"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO4570487.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3056..4429)
                     /gene="dbpA"
                     /locus_tag="ACHJZ2_004509"
     CDS             complement(3056..4429)
                     /gene="dbpA"
                     /locus_tag="ACHJZ2_004509"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO4570488.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4462..4632)
                     /gene="ynaL"
                     /locus_tag="ACHJZ2_004510"
     CDS             complement(4462..4632)
                     /gene="ynaL"
                     /locus_tag="ACHJZ2_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO4570489.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            complement(4907..5890)
                     /gene="zntB"
                     /locus_tag="ACHJZ2_004511"
     CDS             complement(4907..5890)
                     /gene="zntB"
                     /locus_tag="ACHJZ2_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO4570490.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5904..5951)
                     /gene="ydaN"
                     /locus_tag="ACHJZ2_004512"
     CDS             complement(5904..5951)
                     /gene="ydaN"
                     /locus_tag="ACHJZ2_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO4570491.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6145..7377
                     /gene="dgcM"
                     /locus_tag="ACHJZ2_004513"
     CDS             6145..7377
                     /gene="dgcM"
                     /locus_tag="ACHJZ2_004513"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO4570492.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7398..7961)
                     /gene="smrA"
                     /locus_tag="ACHJZ2_004514"
     CDS             complement(7398..7961)
                     /gene="smrA"
                     /locus_tag="ACHJZ2_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO4570493.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         2144 a         2021 c         1901 g         2021 t
ORIGIN      
        1 tttgattaag gttttactta aggcgtaaca aatgatattt atcggctaac tgaacttctc
       61 ctttaggatg ttttcactcc ccgaaatggg gataaaaggc aaataaaata actaaaggat
      121 ttattcaatg aaaagcaaag tactggcact tttaattcct gccctgctcg gcgcaggtgc
      181 tgcacatgca gccgaagttt ataataaaga cggcaacaaa ttagatctgt atggcaaagt
      241 tgatggcctg cattattttt ctgataattc agcgaaagat ggcgaccaga gctatgcgcg
      301 tctgggtttt aaaggcgaaa cccaaattaa cgatcaactt actggctacg gtcaatggga
      361 atacaatatt caggcaaaca acactgaatc ttcaaaaaac cagtcatgga cccgtctggc
      421 atttgccgga ctgaaatttt cagattacgg ttctttcgat tacggacgta attatgggct
      481 ggaccgatat gctgcctgaa tttggcggtg actcttatac caatgcagac aactttatga
      541 ctggtcgtgc caatggcgtc gcgacttatc gtaatactga tttcttcggt ctggtaaatg
      601 gtctgaactt cgcggtgcag tatcaaggta acaacgaagg tgccagtaat ggtcaggaag
      661 gcaccaacaa cggacgtgat gttcgccatg aaaacggtga cggctggggt ctttccacaa
      721 catatgattt aggcatgggc tttagcgctg gtgcggcata cacctcttct gaccgcacca
      781 atgaccaggt taaccatact gcggcgggtg gtgataaagc agacgcgtgg actgctgggc
      841 taaaatacga tgctaacaat atttacctgg caaccatgta ttcagaaacg cgtaatatga
      901 ccccgtttgg cgacagcgat tatgctgttg caaacaaaac ccagaacttt gaagtcactg
      961 cacagtacca gtttgatttt ggtctgcgtc cggcagtctc tttcctgatg tctaaaggcc
     1021 gtgacctgca cgctgccggt ggtgcagaca acccggcagg tgttgatgat aaagatctgg
     1081 ttaaatacgc cgatgttggc gcgacttact atttcaataa aaacatgtcc acttatgttg
     1141 actataaaat caacctgttg gatgaagatg acagtttcta cgctgccaac ggcatctcta
     1201 ccgatgatat tgtcgcttta ggtctggttt atcagttcta aatcctcctg cccgctgtta
     1261 tggcgggctt tttctgctta ttcttcctcc tttgatctaa attaaaaatg tgaactccgt
     1321 cattacacaa aaagtgtcat ctggcgttac actttatgcg gatactaaaa caggaggttt
     1381 tatgaacaga acgattcttg tccctatcga tatttccgat tcagaattaa ctcaacgcgt
     1441 gattagccac gttgaggcag aggcaaagat tgatgatgca gaggttcatt tcctgacggt
     1501 aataccttca ctgccctact atgcctctct gggtttagcg tattccgcag aattaccggc
     1561 aatggatgac ctgaaagcgg aagccaaatc gcaactggaa gagatcatta aaaaatttaa
     1621 actgccaacc gacagagtgc atgtccatgt tgaggaaggc tcgcccaaag accgcattct
     1681 ggaattggcg aagaagatcc ccgctcatat gatcatcatt gcttcccatc gaccggatat
     1741 caccacttat ctgctcggtt ccaacgccgc agctgtagtg cgtcacgcag agtgctccgt
     1801 gctggttgtg cgctgacact aacgcccgca cattgctgcg ggctttttga ttcatttcgc
     1861 aaatgtgctg acattttccc ctctaatccg taccatacac gccacagttt ttatatcaga
     1921 tttcttatgc tgggcgttcc ggcatgacgc atactcttct gatgccatat aacgaattga
     1981 gtcgctttta aatgtcgcaa aatcaagaaa ttagcaagaa agaacaatac aacctgaaca
     2041 aattacaaaa acgcctgcgt cgtaacgtgg gcgaagccat tgctgacttc aatatgattg
     2101 aagaaggcga tcgcatcatg gtttgcctct ccgggggtaa agacagctat accatgctgg
     2161 agattctgcg caatttgcag caaagcgcgc caatcaattt ttcgctggtg gctgttaacc
     2221 tcgatcaaaa gcaaccgggc ttcccggaac acgttctgcc cgagtatctt gaaaagctgg
     2281 gcgttgagta caagattgtt gaagagaaca cttacggtat cgtgaaagag aagattccag
     2341 agggcaaaac cacttgctca ctgtgttctc ggctacgtcg cggtatcctt tatcgcaccg
     2401 caacggaact gggggcgacg aagatcgcgt tgggccacca tcgtgacgat atcctgcaaa
     2461 cgttgttctt aaatatgttc tacggcggca agatgaaagg tatgcctccg aaactgatga
     2521 gcgatgatgg caaacatatc gttattcgtc cgctggccta ctgccgcgag aaagatattc
     2581 agcgatttgc cgatgcaaaa gcgttcccga ttattccgtg caacctgtgc ggttcacagc
     2641 ctaacctgca acgtcaggtg attgctgaca tgttgcgtga ctgggataaa cgttatccag
     2701 ggcgtatcga gacgatgttc agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata
     2761 ccaacctgtt cgatttcaaa ggcatcaccc acggttctga agtggttaac gggggtgatc
     2821 tggcgtttga tcgcgaagag atcccactac aaccggcggg ctggcagcca gaagaagatg
     2881 aaaatcagtt ggatgagtta cggctgaatg tggttgaagt gaaataactc ggatagcgcc
     2941 cgatgcgcaa acgtatcggg ctactctaat ggaggctgaa taagacgcgg tcagcgtcgc
     3001 atccgacaat cccgacgaag atgtttactt ttgcatcagg caattcaaca ttttattatt
     3061 ttaataaccg cacccggcac gtttttcctt taatcttccc gccctgtaac tgtttccatg
     3121 ctttatgagc aacagcctga cggaccgcga catagacatg cgccggatgc acggcgattt
     3181 tgccaatatc tgcgccatca agcccgatat ctcctgtcag tgcacctaat acatcacccg
     3241 ggcgcatttt ggcttttttc ccgccatcga tacacaacgt tgccatttct gcttccagcg
     3301 tcacaatgga actattagct ggtggcgttt gccagttaag ttttatctgc aacatgtcag
     3361 aaatgatatt ggcccgctgt gcttcttccg gagcacagaa actgatcgcc agaccgctat
     3421 ttcctgcacg agctgtacga ccgatgcgat gtacatgaac ttcagggtcc cacgccagct
     3481 caaagttcac caccagctca agcgatttaa tatccagacc acgcgcagca acatcagtcg
     3541 cgaccagtac acgggcgcta ccgttagcaa aacgtaccag ggtctgatca cgatcgcgtt
     3601 gctccagatc gccgtgtaat gacaatgcac tttgccctac ttcattcagc gcgtcgcaga
     3661 cagcctggca atcttttttg gtattgcaaa acaccacgca agaggatggc tgatgcaagc
     3721 ttaataaccg ttgcaacaga ggaattttgc ctttgctgga tgtctcataa aattgttgtt
     3781 caatgggtgg caaagcatct gttgagtcaa tttcaatcgc caaaggatcg cgttgcactc
     3841 gtccgctgat tgcggcgatg gcttccggcc aggttgccga aaacagaagc gtctgtcgag
     3901 atgcaggcgc aaaacggatg acatcatcaa tggcatcgct aaatcccata tccagcatgc
     3961 ggtcggcctc atccatcacc aaagtattca gcgcatccag tgataccgtg cctttttgca
     4021 ggtgatccag caaacggccc ggcgttgcca cgataatatg cggcgcatgt tgcaacgaat
     4081 cacgctgcat accgaacggt tgaccaccgc acaacgtcaa aattttggta tttggcagaa
     4141 aacgcgccag ccgacgcaat tcacctgcca cctgatccgc cagttcacgc gtaggacaca
     4201 gcactaaagc ctgggtttga aatagcgacg catcaatttg ctgtaacaag ccgaggccaa
     4261 aagccgccgt tttgccgctg ccagttttcg cctgcacgcg aacatctttt ccggcaagga
     4321 tcgccggaag cgcggcggcc tgcaccggcg tcatggttaa ataacccaac tcattaaggt
     4381 tcgtgagttg ggcgggaggc aaaacattca gggtagaaaa agcggtcaca atctattctc
     4441 gtggtcatcg acgcaaagtc atcaggcgcg tatcctcgca gatctacgct cacgatgcga
     4501 caatttaatc ggttcttcat cgggtggtgg gtcaggcatg ggttgcgggc gagggatcgg
     4561 atcgggcact ggaacaggat cgccaggaat cggttcaggg acaggaattt gcaaataaat
     4621 aagtgtcgtc atatttccct ccggtcattg ggtggactct taaagggtag acgctgataa
     4681 ataacaggca aaaaaaagcc gactcatcaa agtcggcgtc gtacgaatca attgtgctat
     4741 gcagtaattc aaaaaaggaa gtaagacaat atggagcgca acgcccatcg cttgacgttg
     4801 cattcacctg caagagagat attgccctga atgggtagag agtttattga cttcgctcaa
     4861 actttgcggc gtttttgtat acagacagcc ggaaaaattg cttttgttac aaccatttac
     4921 tacgatgcaa ccataaagca acaccaccaa taagaacaac taacagaata caaaaaattg
     4981 aaaatccgaa ttgccacccg ccgccaggga tcccaccaag gttgacgcca aataaccctg
     5041 tcagaaaggt actgggtaaa aagaccattg ccatcaatga cattgtatag gtacgacgag
     5101 ctaaattttc ctgcatcacc tgagcgattt catccgccat cacaccagtc cgtgctatac
     5161 aggcgtcgat ttcatcaagg ccgcgcccaa ggcgatcggc aatatcctgc atccgacggc
     5221 gttggtcatc gctcatccac ggcaaacgtt cactggcaag acgagcataa acatcacgtt
     5281 gcggtgccat atagcgacgc atcacaatta attgtttgcg cagcagagcc aggaatccac
     5341 gcggcggaat ttgctgatca aggagattat cttcaaggtc gataatttta tcgtgcagct
     5401 gctcgataaa ttcgctggaa tgatcggtca acgcatcgca cacatccacc agccatcccc
     5461 cgcaatcggt cggacccgtg ccctcttcca gatcactcac cacatcgtcc agcgccagca
     5521 ctttgcgttg tcgggtcgaa acaattaacc gcccgtccat atatacacgc atggcgacca
     5581 gttgatcggg gcgttcatcg gtgctgccgt ttatacagcg caatgtaatc agcgtgcctt
     5641 caccgagacg gctgactcgg ggccgtgtgc tctcgcccgc cagcgcatca cgtacgttat
     5701 tgggaagcag cggtgttgtc gccagccatt gggcgctatc atggtgtaca taattaaggt
     5761 ggagccagca gggatgcgct tcatcaatca catctgtatt ttccagcggt ttaacgccgc
     5821 ctctaccatc cagcatccag gcaaatactg catccgggac attaacgtcc gatcccttaa
     5881 tcgcttccac agtgcctcca tcatcaacgc attattttgt agtctagcct tctggccctg
     5941 ttacgcaaca tctcatcacc ccattaccct gaaatgatta ataaaattct gtctaaattg
     6001 aatacaaaaa gcaaaatgct tttccgtata caaaccgtgt gaagtgttaa atagcgtcta
     6061 tcattatcag aattatctga tcatatgacg tggctttttt gcgatcggat agcaacaaaa
     6121 attgataaaa ataatgggat ctcaatgatt acgcacaact tcaatactct ggacttactc
     6181 accagtcctg tctggatcgt ttcgcccttt gaggaacagt taatttatgc caacagcgcg
     6241 gcgcgactgt tgatgcaaga cctcacgttt agtcagctac gaaccggtcc ctattccgtc
     6301 tcctcacaaa aagaactgcc gaaatacctc tccgatctgc aaaaccaaca cgatattatc
     6361 gaaatcctca ctgttcagcg taaagaagag gaaacagcat taagctgtcg gcttgttttg
     6421 cgagagctga cagaaacaga accggtgatt attttcgaag gtatcgaagc gccggcaacg
     6481 ctgggtttaa aagccagtcg ctcggcaaat tatcagcgca aaaaacaagg tttttatgcg
     6541 cgcttttttc tgactaactc tgcaccaatg ttgttgattg acccgtcacg agatggacaa
     6601 atcgtcgatg ctaacctcgc cgcgctcaat ttctatggtt ataaccatga aacgatgtgc
     6661 cagaaacata cctgggaaat aaatatgctc gggcgtcgcg tcatgcctat catgcatgaa
     6721 atctcgcatt tacccggtgg tcataaacct ctgaatttta ttcataagct ggcggatggt
     6781 tcgactcgtc atgtgcagac ctatgccgga ccgattgaaa tttatggcga caagctcatg
     6841 ttatgtattg tgcatgatat tactgagcaa aaacggctgg aggagcagct ggaacatgct
     6901 gctcaccatg acgcgatgac cggattactg aatcggcgac agttttatca cattacggaa
     6961 ccaggccaaa tgcagcacct cgccatcgct caggattaca gcttattgct catcgacaca
     7021 gatcgtttta aacacattaa cgatctctat gggcattcta aaggtgatga ggtgttatgc
     7081 gccctcgccc gcactctcga aagttgcgct cgcaaaggcg atttggtgtt tcgttgggga
     7141 ggcgaagagt ttgtcttatt actaccaaga accccactgg ataccgcgct ttcgctggct
     7201 gaaactatcc gcgtaagcgt ggcaaaagtg agtatttcgg gcttaccacg ctttaccgtc
     7261 agcattggtg tggcgcatca cgaaggaaat gaaagcatcg atgaactgtt taaacgcgtt
     7321 gatgatgctt tgtatcgggc gaaaaatgat ggacgcaacc gcgtgctggc ggcataagcc
     7381 acggatgcgt ctcgagatca acgactgcgc ttagcgtggc gctcccagtt ttcttgcttt
     7441 gcctgtgccg ttttacgtag tgcgacgtaa cacgccccgc tgccgccatg atgcggtagc
     7501 gcggtacaat atgcctggac atcatcaaat tcggtcaacc agcgcgccac atagctgcgg
     7561 acaatattgg catgcgattt atcatcccgc cctttaccat gaataatcag cacgttacgc
     7621 aaaccatccg ccatggcttg ttgaatgaaa ctgaacatca ttttgcggca ctcttccacc
     7681 ggctggcgca aaagattcag gctcgcctgt tgcggatatt taccactgcg cagcttatcc
     7741 agcaccccat gttgcaaccc ttcccgccga aactccagcg gctgacttag cgggatgatg
     7801 tcgagaaatc cggtggtgag gaaattatca agttgcagcg tgtcgatacg ctgcggcgca
     7861 cgttggttac gcgttggatg ccagtggaca tcggtagcac gtttcagcgg ctggacatct
     7921 tccatggcgt caagaaacag cgatttgtcg tcaaggttca tgttacatcc tcccgcaatt
     7981 aagagcgcga tatgataacc agaccgggtc ggtccaacaa cgtattaccc aaaattccag
     8041 taataagttc caaatattgc cgatatttta agcaaaatgc ttatgct
//
LOCUS       DAZFBR010000170         8072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFBR010000170 DAZFBR010000000
VERSION     DAZFBR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.170"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..224
                     /locus_tag="ACHJZ2_004515"
     CDS             <1..224
                     /locus_tag="ACHJZ2_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570494.1"
                     /translation="PVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTPETL
                     FGSKSYDKHALFPIFTASWRANRIKNKGI"
     gene            447..944
                     /gene="ubiC"
                     /locus_tag="ACHJZ2_004516"
     CDS             447..944
                     /gene="ubiC"
                     /locus_tag="ACHJZ2_004516"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO4570495.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            957..1829
                     /gene="ubiA"
                     /locus_tag="ACHJZ2_004517"
     CDS             957..1829
                     /gene="ubiA"
                     /locus_tag="ACHJZ2_004517"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO4570496.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1984..4407)
                     /gene="plsB"
                     /locus_tag="ACHJZ2_004518"
     CDS             complement(1984..4407)
                     /gene="plsB"
                     /locus_tag="ACHJZ2_004518"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO4570497.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4578..4946
                     /gene="dgkA"
                     /locus_tag="ACHJZ2_004519"
     CDS             4578..4946
                     /gene="dgkA"
                     /locus_tag="ACHJZ2_004519"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO4570498.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5056..5664
                     /gene="lexA"
                     /locus_tag="ACHJZ2_004520"
     CDS             5056..5664
                     /gene="lexA"
                     /locus_tag="ACHJZ2_004520"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO4570499.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5737..7062
                     /gene="dinF"
                     /locus_tag="ACHJZ2_004521"
     CDS             5737..7062
                     /gene="dinF"
                     /locus_tag="ACHJZ2_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO4570500.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7178..7387
                     /gene="yjbJ"
                     /locus_tag="ACHJZ2_004522"
     CDS             7178..7387
                     /gene="yjbJ"
                     /locus_tag="ACHJZ2_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO4570501.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7429..7944)
                     /gene="zur"
                     /locus_tag="ACHJZ2_004523"
     CDS             complement(7429..7944)
                     /gene="zur"
                     /locus_tag="ACHJZ2_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO4570502.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1811 a         1982 c         2227 g         2052 t
ORIGIN      
        1 ggccagttat ggtggtaagc tcccagcgct tacatgatat gttgaatcct acaaaagata
       61 ccaattggaa ttccacgtat atctataaat ccagacatga gatgttgcct gttaatctta
      121 ctccggaaac acttttcggc tccaaatctt atgataaaca tgcgcttttc cccattttta
      181 ctgcgagttg gcgagctaat cgtataaaga ataaaggtat ttaagtaaag gaaaacatca
      241 ccgctcttgg catcatgggc ggtgatgccc ctatggttgc cctcgccagc acgggcatcg
      301 gcaaagcgta aggttcaaca gcgttttacc acttcatgcg attgttgcgt ttttgttgcg
      361 cattagatca cttaatttgc tttacatctc ccgtaaacac ttttctgcga tacaatgcct
      421 ttacgttatg taacggagag ttcggcatgt cacaccccgc gttaacgcaa ctgcgtgcgc
      481 tgcgctattg taaagagatc cctgccctgg agccgcaact gctcgactgg ctgttgctgg
      541 aagattccat gacaaaacgt tttgaacagc agggaaaaac ggtgagcgtg acgatgatcc
      601 gcgaagggtt tgtcgagcag aatgaaatcc ccgaagaact gccgctgctg ccgaaagagt
      661 ctcgttactg gttacgtgaa attttgttat gtgccgatgg tgaaccgtgg cttgccggtc
      721 gtaccgtcgt tcctgtgtca acgttaagcg ggccggagct ggcgttacaa aaattgggta
      781 aaacgccgtt aggacgctat ctgttcacat catcgacatt aacccgggac tttattgaga
      841 taggccgtga tgccggactg tgggggcgac gttcccgctt gcgattaagc ggtaaaccgc
      901 tgttgctaac agaactgttt ttaccggcgt caccgttgta ctaagaggaa aaaaatatgg
      961 agtggagtct gacgcagaat aagctgctgg cgtttcatcg cttaatgcgt acggataagc
     1021 caattggcgc gttactgctg ctctggccaa cattatgggc gttgtgggtg gcgacaccgg
     1081 gcgttcccca gctctggatc ctggcggtgt ttgtcgcggg tgtctggctg atgcgcgctg
     1141 ccggatgtgt ggtgaatgat tatgctgacc gcaagtttga tggtcatgtt aagcgcacgg
     1201 cgaaccgacc acttcccagc ggcgcggtaa cagagaaaga ggcgcgcgcg ctgtttgtag
     1261 tgctggtact gatttcgttt ttactggtgc tgacgctgaa tacgatgacc attctgttgt
     1321 cgattgccgc gctagcgctg gcgtgggtgt acccgtttat gaagcggtat acccatctac
     1381 cgcaagtggt gctgggcgcg gcgtttggct ggtcgattcc aatggctttt gctgctgtga
     1441 gtgagtcggt gccattgagt tgctggttaa tgttcctcgc caatattctc tgggcggtgg
     1501 cttacgacac gcagtatgcg atggttgacc gtgatgatga tgtgaagatt ggcattaaat
     1561 ccaccgcaat cctgttcggc caatacgata aattgattat tggtattttg cagattggcg
     1621 tactggcgct gatggcgatt atcggtgagt taaatggctt aggctgggga tattactggt
     1681 caattctggt ggctggcgcg ctgtttgttt atcaacaaaa actgattgcc aaccgcgagc
     1741 gcgaagcctg ctttaaagca tttatgaata ataactatgt tggtctggta ctgtttttag
     1801 ggctggcaat gagttactgg catttctgat gatgtaaaaa agccggatga tcatccggct
     1861 ttcttctggg ttgcctgatg cgctgcgctt ctcaggccta cacaacagat cgcaatttat
     1921 tgaatttgca gattatggaa ggccggataa ggcgtttgcg ccgcatccgg caattctctc
     1981 tgattaccct tcgccctgcg tcgcactctc aatcgtcaaa cgcacgtctg atgtaatcaa
     2041 ctccgccagc aactgataaa ccttcatcgt ttctgccggt tcggcatcgc cgctatcgct
     2101 gatataccct tcatcacgca gcgtaagcac cagagaactg aacaccgcct tgtcgaagaa
     2161 ctccggcgcg ttgatgccgt gcagcacgga gagacgttgc gcgacggtgc ggctctcttt
     2221 ctccagcgta ccgcggttga tcgacgggtt ggcgctcaac agccagaagg tgatggcata
     2281 acgttgcagc gtttcgcgcg cgcctgcggc gagcagctgt agcgtgcgag aatgcaccgg
     2341 gttgatatgc aactcatcat cttgcagggt aatcagcccc tgacgttgca tctcatttgc
     2401 cagcgcatca ataacgtccg gcaactcgtc gcgatcccag cgcaggaaca gctccgcttt
     2461 cagcattggg taaagcacat tgacgtgctc catcaatacg tcgcgggaga tgtggcgatg
     2521 ctgggtgaca attgccgcca tcagcgaagg tagcaccaac atatgcgcaa tgttgttgcg
     2581 atagtaggtc atcagcaccg cttgctcgcg cggcagaatg atgatgtcgc cgatagtgtc
     2641 tttctcaact tcaaacttgt tcatttgcag cgcgtgatcg ataagctcgc tggcgctggc
     2701 tgaaggaacg gtagagtccg tggagtaagg cacgttgcgc atcagatcca ggtagcagtt
     2761 gagttgctcg gttaactgct cgcgggtgag tgagcgctga cgtgatgcca gtagcgcagt
     2821 acagcacagg ttcatggcgt ttgccgcgcc tgcgttgtta atgcgtacca tcagatcggc
     2881 agcaatatta ttgaccgtcg gcgttaacca tgccggacgc actgcttcga tgggatcgat
     2941 agattcacgc cagtctggta cgtgctggtt aaggtaggtc atcaacggca ttggttcacc
     3001 gaaattgacg taaccctggc cgagattacg cagcttgctt aaaccgcgca gcatctgcgg
     3061 caggctctct ttctctttcg tcgcgccgcg cagttctttg gcgtaagtac ccacttccat
     3121 gacgtgctca taaccgatat agatcggaat cagcgtaatc ggacgcgtgc cgccacgcag
     3181 catcgcctga atggtcatcg acagcgtacc ggttttcgga tccagcaaac gccccgtacg
     3241 ggaacgaccg ccttctacga agtactcgac ggaataacca cggctgaaca gttcgccgag
     3301 atactcacgg aaaacggtgg aatagagctt attgccttta aaagtacggc gaataaagaa
     3361 cgcacccaga cggcggaaaa tcggcccggc aggccagaaa ttcaggttga tcccggcggc
     3421 gatatgcggc ggcactagcc cctggtggta cagcacgtaa gaaagcagca ggtagtccat
     3481 gtgactgcgg tggcaaggca catataccag ctcatgaccg tcgtgggcca gctggcgaac
     3541 gcgctcggcg ttatggacgt tgatgccctg gtaaaggcgg ttccaggtaa agcccagaat
     3601 acggtcagtc aggcggatca tctcgtaaga gaaattcgcc gcaatctctt ccatcagcgc
     3661 aatcgcgttc tgctgcgctt tttcatggga gatttttttg ctgcgcgctt catcttctac
     3721 cgctttggca atggcgcggg aggcgagcag cttattaaac agatcctgac gagcaggcag
     3781 acgtggtcct acggcagcca gacgttgacg ggcaaagtgc atacgcgcca cgcgcgccag
     3841 tttctgcgcg atagttttat ccgtgccgtg ttcatccgcc atacggcgca gcgaaactga
     3901 cggcgagaaa cgcacaaaac tgtcgcgacc gagccacagt acagcgaaaa atttctgtac
     3961 gccgttaagc atacgcagcg gcgggttcac ttcgcctttt tcacgccccg gcgcgcgacc
     4021 aaacatcacc gacactggca ccatctgcac atccagattt gggttgctac ggtgcaaatc
     4081 gagatagtcg tggaacagct taatagactc ttctttcggc gtgtaatagg tgaatacacg
     4141 cggcccgccg tgaatgaaca catagcgcgg cagtagcgtg ccgtcgattt ccagcggctc
     4201 taacgggtca ggcaagtcat gtgccagaca ctgggcgcgc aacgtcagca aatctgcttt
     4261 cgagttgtac ggtaaaacgt acataattgg acgagaggta tccagcccca gttccggggc
     4321 aggatctgcc ggaatagact tgctttttac caggatgctt aatggtaaat tcagtaattt
     4381 gtagtaaatt cgtggccagc cggacataaa cgatgtaaag cctctggtta ataatgcaaa
     4441 tgcgcggcaa ggatagcaga aagtcatggg aaattctgtg gtatccgctc atgtttcgcg
     4501 cggcgttacg caaacccgaa tcatcggatt taacggtaca ctgatattga cgctcataat
     4561 gtaaaaaggt tctttcaatg gccaataata ccactggatt cacccgaata atcaaagctg
     4621 ctggctattc ctggaaaggt ttacgcgctg catggatcaa cgaagcggca ttccgtcagg
     4681 aaggcgtagc ggtattgttg gcggtggtca tcgcctgctg gctggatgtg gacgcgatta
     4741 cccgcgtgct gcttatcagc tccgtgatgc tggtgatgat tgtggaaatc ctcaatagcg
     4801 ccatcgaagc agtggttgac cgaattggct ctgaatacca tgagctttcc ggacgcgcta
     4861 aagatatggg atccgccgcg gtgctgattg cgattatcgt cgccgtgatt acctggtgca
     4921 tcctgttatg gtcgcatttt ggataaccct tccagaattc gataaatctc tggtttattg
     4981 tgcagtttat ggttccaaaa tcgccttttg ctgtatatac tcacagcata actgtatata
     5041 cacccagggg gcggaatgaa agcgttaacg gccaggcaac aagaggtgtt tgatctcatc
     5101 cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg
     5161 gggttccgtt ccccaaacgc ggctgaagaa catctgaagg cgctggcacg caaaggcgta
     5221 attgaaattg tttccggcgc atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg
     5281 ttgccactgg taggtcgtgt ggctgccggt gaaccgcttc tggcgcaaca gcatattgaa
     5341 ggtcattatc aggtcgatcc ttccttgttc aagccgaatg ctgatttcct gctgcgcgtc
     5401 agcgggatgt cgatgaaaga tatcggcatt atggatggcg acttgctggc agtgcataaa
     5461 actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga agttaccgtt
     5521 aagcgcctga aaaaacaggg caataaagtc gaactgttgc cagaaaatag cgagtttaaa
     5581 ccaattgtcg ttgaccttcg tcagcagagc ttcaccattg aagggctggc ggttggcgtt
     5641 attcgcaacg gcgactggct gtaacatatc tctgagaccg cgatgccgct tggcgtcgcg
     5701 gtttgttttt catctctctt catcaggctt gtctgcatgg cattcctcac ttcatctgat
     5761 aaagcacttt ggcatctcgc cttacccatg attttctcca atatcactgt tccgttgctg
     5821 ggactggtcg atacggcggt tattggtcat cttgatagtc cggtttattt gggcggcgtg
     5881 gcggttggtg caacggcgac cagctttctc tttatgctgt tgctgttttt acgcatgagc
     5941 accaccgggc tgactgcgca ggcttatggt gccaaaaatc ctcaggcatt agcccgtgcg
     6001 ctggtgcaac ctttgctgtt ggcgttgggg gctggggcgt taattgcgct gctgcgtacg
     6061 ccgattatcg atctggcgct gcatattgtt ggcggtagtg aagcagtcct ggaacaggcg
     6121 cggcgctttc ttgaaatccg ctggttaagc gcaccggcgt cgctggcgaa tctggtatta
     6181 cttggttggt tactcggcgt gcaatatgcc cgtgcgccag taattttgtt agtggtcggc
     6241 aatatcctca acattgtgct ggatgtctgg ctggtgatgg gactgcatat gaacgtgcgg
     6301 ggcgcggcgc tggcgacggt tattgcggaa tatgcaacat tgctgattgg tctgctaatg
     6361 gtgcgtaaaa tcctcaaact acgcggaatc tccggcgaaa tgctgaaaac ttcctggcga
     6421 ggaaacttcc gtcgcttgct ggcacttaac cgcgatatca tgctgcgctc gctgttgttg
     6481 caactctgtt tcggcgcgat caccgtactt ggcgcgcgac tggggagtga cattatcgct
     6541 gttaacgcgg ttctgatgac gctactcacc tttaccgcct atgcgctgga cggttttgcc
     6601 tacgcggttg aagcacattc cggtcaggca tacggtgcgc gcgacggtag ccagttgctg
     6661 gatgtctggc gggcagcatg ccgccagtcg gggatcgtag cgttactgtt ttcggtggtt
     6721 tatttgctgg caggggaaca catcattgcg ttactggcgt cgttaaccca gattcagcag
     6781 ctggccgacc gctatcttat ctggcaggtg attttgccgt tggtcggcgt ctggtgttat
     6841 ctgctggacg gcatgtttat aggcgcaacg cgtgctgctg aaatgcgtaa cagtatggcg
     6901 gtggccgccg caggttttgc gctgacgctc cttacgctgc cgtggctggg taatcatggt
     6961 ttgtggttgg cgttaaccgt ctttctggcg ttgcgcgggc tttctctggc agctatctgg
     7021 cggcgtcact ggcgcaacgg tacctggttt gccgcaacgt gacggttaaa aattctgaat
     7081 aaataatcct aagccaaatt gctgactaca cttaatctca cgttcagaag aaaagtgaac
     7141 gtactctcat tcacaaccta acgatgaggt cttgattatg aataaagatg aagccggcgg
     7201 taactggaaa cagtttaaag gtaaagtgaa agagcaatgg ggcaaactga ccgatgatga
     7261 tatgacgatc attgaaggta aacgtgatca actggtcggt aaaatccagg aacgttatgg
     7321 ttatcagaaa gatcaggcag aaaaagaggt cgtggattgg gaaacccgca atgaatatcg
     7381 ctggtaatta atccctcctg cccgacgtgt acaaggatgt acgccctctt aacgcggttt
     7441 ctttttcacc tgcacagagt gatcatgctg gcactgttca ggatgacgac acgcttccac
     7501 ttccacgcat gccgcacaga gcccatgtgc ttcaatcaca ttatgccgca gggcaaaccc
     7561 catttttgcc gccagcgtat gcatgatgtc ttccacgcct tcagcacatt cttctttcac
     7621 cgcgccgcag cgatcgcaaa taaacatagc tgacgtatgg gtgggctggt cgaacagatg
     7681 gcagagcaca taactgttgg tggattccac cttatgcaca aaaccttgct caagcagaaa
     7741 atccagcgcg cgataaaccg ttggcggctt ggcttgcggt tcagcttcgc gcagcaaatc
     7801 aagcagatca taagcgctga tagcgccatc ctgcagactc atcaggcgca acacttccag
     7861 gcgctgtggg gtcaggcgca cattacgctg cgcgcagatt ttgtcagcct gcgctaataa
     7921 ctcctgcgtt gtggtctttt ccatcgggca cctcaaagtg ggggttaaga atgtccttac
     7981 tttaccatgt tccaggaaaa acaccgagaa cccttattgt tgccgtaatg ttgcttttct
     8041 gttttgtagg taagatgttg tgttgcctgg ca
//
LOCUS       DAZFBR010000171         8018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFBR010000171 DAZFBR010000000
VERSION     DAZFBR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.171"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(142..999)
                     /gene="murI"
                     /locus_tag="ACHJZ2_004524"
     CDS             complement(142..999)
                     /gene="murI"
                     /locus_tag="ACHJZ2_004524"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO4570503.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(944..2788)
                     /gene="btuB"
                     /locus_tag="ACHJZ2_004525"
     CDS             complement(944..2788)
                     /gene="btuB"
                     /locus_tag="ACHJZ2_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO4570504.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGGSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2853..3043)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3157..4257
                     /gene="trmA"
                     /locus_tag="ACHJZ2_004526"
     CDS             3157..4257
                     /gene="trmA"
                     /locus_tag="ACHJZ2_004526"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO4570505.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4297..4656)
                     /gene="yijD"
                     /locus_tag="ACHJZ2_004527"
     CDS             complement(4297..4656)
                     /gene="yijD"
                     /locus_tag="ACHJZ2_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO4570506.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4656..5306)
                     /gene="fabR"
                     /locus_tag="ACHJZ2_004528"
     CDS             complement(4656..5306)
                     /gene="fabR"
                     /locus_tag="ACHJZ2_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO4570507.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5410..6726)
                     /locus_tag="ACHJZ2_004529"
     CDS             complement(5410..6726)
                     /locus_tag="ACHJZ2_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO4570508.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6769..7935)
                     /locus_tag="ACHJZ2_004530"
     CDS             complement(6769..7935)
                     /locus_tag="ACHJZ2_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO4570509.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2107 a         2176 c         1898 g         1837 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagagg aaatttaaaa taattttccg
      121 accgcgcaac attcaaccaa atcagcctaa aactgccagt ttttcgagcg tttcgaagcc
      181 gtaacgctgt aaaacgggca ataattgttc agcttctggc gtcattgcca tacaaaaggc
      241 aatattcgca tcggcagatt ttgcatccgg ggcttcatgt tctaacaacc aggccgttcg
      301 gcgagcaatc gctgcgccag aatccaccag ccgggttccc tctggcagca cttgtaacag
      361 ttcttcttgt agtagaggga aatgggtgca acccaataca acggtatctg gcggctcttt
      421 cattcttaac cacgggcgta ggatacgttt tagtgcatcc agagaaacat cttcgccatg
      481 cagcttcgct tcagccaact caaccatctc tgccgagccc agcatttcta tctggcattc
      541 attagcgaaa cgcgcgatca gctcatgagt ataagaacgt ttaactgttc cgcgggttgc
      601 cagtaatccg acaatgccat ttgccgtcag acgtgcagca ggtttaatcg ccggcacgac
      661 accaacaacc gggaagtcga acttttcgcg taatgcagga agtgaaacgg tactggcagt
      721 gttgcaagcg accacagcca gcgcaagggg ataacgttct tgcaccgcgg tgacaattgc
      781 caccactcgc tcaacaataa acgcttcgct tttttcgcca tacgggaaag cgacgttatc
      841 gaaagcataa atgtaatgga gatccggtaa gagatgccgg atctcgtcat agaccgacaa
      901 cccaccgacg ccggagtcaa acaccagcac ggtgggacgt ggttcagaag gtgtagctgc
      961 cagacaaggt gtattcccgt cctgcagttt ggtagccata gactgtctca taatctttgt
     1021 cgaacaggtt ggctatttta ccacgaactg tcaggtgaga ggtgaccgga tacgcaaccg
     1081 caagatccca caagctcaca ccgcccattt taacggtttg ataaggataa gatgagtaat
     1141 ccttatcata gcgagtgcct aaatactgat aagtaatacc ccagtcgaag tcatacaact
     1201 gccagtcgag ctggtatttc acctgctgtt tagcacggcg taacaacggc gtgtcggtaa
     1261 ttgcattgcg cgcatcgaca taatcataac tcacagtatg cgtcagtggt ccggtatcaa
     1321 aattggcggt cgcctcgacg cccttaatcc gcgctttccc ttcgttgtaa tatttcaggg
     1381 tgtgatcatc ataatcgatc aagtcactga catcgttacg atatccggaa atacgccagt
     1441 tcaccccagc ggttaagcct tcaaacgcgc cttcccactg tttgcttttc tccgggtcca
     1501 gattcggatt tccgtagaag ccatacagtt gccccagatt tggtgcctta taagatgtcc
     1561 cgtaggaagc aatgaagcga taaccttcga tgaattccca accggcgctg gtttgccagg
     1621 ttccatgacg accaaactgt gagttatcgt cgctgcgtgc tgcgccttca aaggtaaaat
     1681 cgccgacttg ttgcagcccg gtcagataga tgccggtatt acgttgatca tatccatcct
     1741 taacataagc tgtgcccggt gccgtgctct gcttctgcca gtcaacaccc gcaccaacat
     1801 taccgtggcc aatgatgatg ttgtttgccc actggacggt gtattgcttc atctcatcga
     1861 gcgtcgccga cgaatcataa cgaccataat ggggatcgta gttgtaatct ttgctatggc
     1921 tatagctggt aatgagttgt gatttaatca gttcgccgtt atatcgcagc ccggcgtccc
     1981 aactttgact atagagttta cgggtatcga ccaatggtga acccggagaa taatacgcgt
     2041 cataattggt acggttatca tagccatagc cgcgcacaaa gccgctccag gcatcagtaa
     2101 agttatgctc cagcgcgcca taaagcgttt tacttaaaaa accatcgtta tctggctgcg
     2161 cttgcgttcc ggtattacca taggcaacaa catcataacc atgagtatgg gcataatcgc
     2221 ccaacaacgt tactcgtgtc ttatccccca gttgttgctg tgtagagaca tcatagtttt
     2281 gataactatt gcttccccac cctgctgaaa tttccgttcc gggttcatcg cgcgtcgtga
     2341 tgatattcac caccccacct attgcatcgg aaccataaac ggcggagcgt ggcccacgga
     2401 tatattcaac acgctggaca agcgcaatag ggaactggct aaggtcggca gaaccactcc
     2461 cccccgccag attcaggcgt acgccatcaa ttaacaccaa cacatgactg gcatttgtac
     2521 cgcgaataaa aatagatgag agctgacctg aaccgccgtt ttgggtgata tcgacgcccg
     2581 gaagacggcg cagcacatca ttaaccgagg tcgactgcca gcggtcgata tcctgacgcg
     2641 tcacaacggt ggttggtgca agcacagtgc tgcgcggctg ttcaaaacgg ttagcagtaa
     2701 cgacgagagt atccgggctg gtatcctgtg cccaagcgga aaaggctgtg acagaacacg
     2761 ccgtcagcag cgaagctttt ttaatcattg taaagcatcc acaatagaag aaggatgccg
     2821 caggtttcat caatattacg cgatgatgag aaccagatgc gacgttggcc ggcaggtctt
     2881 cgggcttgga ggggtatcta agatactaag agatgatgac ttcccaccga atggcagtgt
     2941 ccgcataacg caatcatcgc acctttcctt accgctgcgc gtcagctcca gattcgcact
     3001 ggattcccta ttaactcaca ggaccggcaa gtggatgcta caggttgtaa caagttactg
     3061 tccagacgta gctcacaaat aggaattcat caagatctgg acatctgatg agcaatccct
     3121 acaatcgccg cgtactttaa tttttcagga tacatcatga cccccgaaca ccttccaaca
     3181 gaacagtatg aagcgcagtt agccgaaaaa gtggtacgtt tgcaaagtat gatggcaccg
     3241 ttttctgacc tggttccgga agtgtttcgc tcgccggtca gtcattaccg gatgcgtgcg
     3301 gagttccgca tctggcacga tggcgatgac ctgtaccaca tcattttcga tcaacaaacc
     3361 aaaagccgta tccgggtgga tagcttcccc gccgccagtg aactcatcaa ccagttgatg
     3421 acggcgatga ttgcgggtgt gcgtaataat cccgttctgc gccacaagtt gttccagatt
     3481 gattacctca ccacgctgag taatcaggcg gtggtttccc tgctgtacca taaaaagctg
     3541 gatgatgagt ggcgtcagga agcggaagcc ctgcgcgatg cactgcgcgc gcagaatctg
     3601 aatgtgcatc tgattggtcg ggcaacgaaa accaaaatcg cgctggatca agattacatc
     3661 gacgagcgtc tgccggtcgc agggaaagag atgatctatc gtcaggtaga aaacagcttt
     3721 acccagccga acgcggcgat gaatattcag atgctggaat gggcgctgga cgtaaccaaa
     3781 ggctcaaaag gcgatttact ggagctgtac tgcggcaacg gtaacttctc attagcgctg
     3841 gcgcgcaatt ttgatcgggt attagccacc gaaatcgcca agccatcagt cgctgccgcg
     3901 caatacaaca ttgccgctaa ccatattgat aacgtacaaa ttattcgtat ggcggcagaa
     3961 gaatttactc aggcgatgaa tggcgtgcgc gagtttaacc gcctgcaagg gatcgactta
     4021 aagagttatc agtgcgaaac catttttgtc gacccacctc gcagcggtct ggacagtgaa
     4081 accgagaaaa tggtgcaggc gtatccacgt attctgtata tctcctgcaa tccggaaacg
     4141 ttatgcaaaa atctggaaac attaagccag acgcacaagg tcgaacgtct ggctctgttt
     4201 gatcagttcc cctacacgca ccatatggag tgcggcgtat tactgaccgc gaagtaaaac
     4261 ccatgccgga tgcgccagca tccggcatga taccgattac tcagcaactt cctgcttacg
     4321 gttacgcatc ttcgcgccaa tccagaacac cataatgacg gagagtactg ccgggaagaa
     4381 gttagagccg atatccggat attccgcacg aactaccgtg ctgtacagca gtacgccgag
     4441 aataaaacag gcagctgcca gacccggcaa gcctaccggc atggtgcgat taagataacg
     4501 ttgatgcagg cagtaaaccg tcagcaccag ggaaataatc gggaatacag aaaatggcac
     4561 aatggagcta aacagcgctg cgaaagtacc attaatcgat aagccagcaa ccaacgccag
     4621 cagcagcgta cctctatctt gatttgcttg tttcattact cgtccttcac atttcccgga
     4681 ataattgcgg ttttctcttg ttcacggcga taccagtaat aagccccttt cgaaatcatt
     4741 cgcagttgca gtaccagtcg ctcttctaat tgccgacgtt gttcaacgcc gacgtccaac
     4801 gcctcggcac ccgcactgaa gacaattgtc accattgctt cggcttgcgc ttcagtaaac
     4861 gcacgcggca tatggttttc gagttccaga tagtccgcaa gttccgcaat gaagtgctga
     4921 atttcacgcg caacggcggc acgaaacgca gcggaggtgc cggagcgttc ccgcaataat
     4981 aaccggaagg cgttaggatt attaccgatg aactccataa atgtggagac cgaggtgcgg
     5041 atcacactcc cgcctttggc gatacgctga cgcgcctggc gcatgagttg gcgtagcatt
     5101 aaaccgctct catcaaccat ggtcagaccc agttcgtcta cgtctcggaa atgccgataa
     5161 aaagaggtgg gagcaatgcc cgcttcacgc gccacttcac gcaaactcag gctggcgaag
     5221 ctgcgttcag cacttaattg gctaaatgcg gcttccacca gcgaacggcg ggttttttct
     5281 ttttgttgcg ctcttacgcc catcacgatg tctgaatcct tgccaaaagt actgctggca
     5341 ctataccaga gaatgaacat actggattgc tccagtattg tgaatgaacg gtaacgcaat
     5401 aaaacatatt tactgcaatt ctttggccgg ttcttttacg tacaacaacg ccaacatcga
     5461 cagcaacgtg cctccaccca gataccacgc cggagccgtc aaactatcca gcaacacgat
     5521 ggattgcgta gcgaaatatt gagcaaaacc gccaaagatt gccacaccaa tgctataaac
     5581 cagcgcaaaa cccaatgccc gaatccgttt cggcaacagt tcagagatca acaacatcac
     5641 tggcacaccg ccaagcgtgg taaagctgac catcacgaaa accaccgtca gcaacatgcc
     5701 tgggcttggg taattcacca acagccagaa tgacggccag ctacagatca gcaccatcac
     5761 acgggagatt aaaatcagct ttttacgccc gaccgaatcg cacaacatcc ccaccagcag
     5821 cgcacccaca aacgtaataa cgcccgccag taacatcgct gcatgagaat agttttggtt
     5881 cattcctaaa tacttcgccg cccatgtacc gtaataaaag agggagatat aggttgcgac
     5941 tgtactacca atagcgagca agataccgtt agcgatagtc gctttatgtt gcataagtaa
     6001 ggagaaggcg ctttcgctgg ctgcggcctt cttattatgt gctggctcag gaacatcatt
     6061 ttccagactc aaacgcaacc aacagccgac aggcgctaat aacacgccaa taaaaaacgg
     6121 cacacgccag ccccattcag ccatgacggt ttctgaaccg gtcgcaaagg gcagccatgc
     6181 acttaaacct aaagcgacaa cacctccaaa agttgttgcc aggccttgag tcgccagtga
     6241 ccaactgctg taaaaaccgc gacgatttgc cggagctgat tcaaccaaca aagacattga
     6301 ggcaccgact tcgcctcctg ccgcaacgcc ctgaattaga cgcgctagca ccagcgtcgc
     6361 ggttccccaa tagcccgccg tttcatacgt cggtgcgatg ccgatcatca gtgtaccaag
     6421 actcatcagc gcaatggtga acaccatcgc gggtttgcgt ccgaaacgat cggcccaggc
     6481 ccccacaata atgccgccga gcgggcgcat aaaaaagctc acgccgaaag tggcaaacgc
     6541 cagcagtagc gcgacgtttt tatctgcact gggcggaaaa aatagcgtgc tgatatagac
     6601 cacaaaaaag ttgtaaacgc caaaatcaaa aaattcgagc gcattgccaa acgccgaggc
     6661 aaaaatcacc cgcctgccgc tgggcttttt cttcacactc acggtttcat ttacgatcgc
     6721 cgtcattcca tgttccttgc caaaaagtgt tgttgtgtgt tgttttattt attgcagcca
     6781 ggcttctacc agtgcgcccc agtagctgct tgccgggacc agacttgcat cgttgaagtc
     6841 gtaacccggg ttatgcacca tgcaggaacc ttccccgaca tcgccattac cgaataacag
     6901 gaaaccgcct ttgggttggg cttccagcat gaaagagaaa tcttcactgc ccatcagggg
     6961 cttgatgcca aactcggccc gatcttcacc aaacgtttta cacgccacct gccaggcgaa
     7021 gcgcgccatc tcctcgtcat ttactaatac tggtgtaccg ttaacatgcg taacctcagc
     7081 cgtagcgcca aagctggcag cctgggcttg tgccagagca ggaatacggg tcagaagaag
     7141 ttgtcgggta tcgcggctaa gagagcggac gctgattttc atctcagcac tgtcaggaat
     7201 gacgttagcc gcctcacccg caacaatgct gccgacggta attaccgccg cttccagcgg
     7261 atcgacattg cgtgacacaa tgctttgtaa tgcggtggtg atatgtgcgg caactagcac
     7321 cggatcgatg gctttgtgcg ggatcgcacc gtgtccgcca caaccacgaa ctgtaatatg
     7381 gaactgatcc attgacgcca tcagtgcccc tggctggaaa taaaacttac ccaccggcag
     7441 accgggcatg ttatgcatgc cgaaaatgac atcgcagggg aaatgatcaa aaagcccctc
     7501 tttaaccatg atctcaccgc cattaatcat ctcttccgca ggctggaaga taagacgtaa
     7561 cgtgccgtta aaacggcggg tttcggcaaa ataacgcgcg gcccccagca gtatggtggt
     7621 atgcccgtca tgcccgcagg cgtgcattaa accaggatgt ttactggccc agggcttgcc
     7681 actattttca taaatcggca gcgcatccat atccgcccgc aggccaatac ttttctctcc
     7741 gtcgcccact tttaacgtgg ccactatccc cgtcgtcgcc agtccttgct ctacttcata
     7801 gccccactga cgcagctttt ccgcaaccaa agcactggtt ttaaattctt gtaatcccag
     7861 ctccgggttt tcgtggatct gatggcggat ctcacgcagt tcttcctcaa accctcgaat
     7921 atagtgttcg atcatcggct attcctttat tcacttataa acaggctaat taacggattt
     7981 tattgatggt gtaaatacaa ataactcatc aatataaa
//
LOCUS       DAZFBR010000172         7987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFBR010000172 DAZFBR010000000
VERSION     DAZFBR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.172"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..542
                     /locus_tag="ACHJZ2_004531"
     CDS             <1..542
                     /locus_tag="ACHJZ2_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570510.1"
                     /translation="SSLQDKEDDVDELKYSAEDFDSLTVADLYDIEIAMQDFLNDINF
                     ENSKDNKVRFDEDTYDFNINGKRRGMFGKGTRAVMHAIFTICFAEFLSRKGNPFIGFV
                     VLDSPLVTHFDKDRGGSLSDVNSVSLSDSFYHALIKRDYNFQIVILENKGPTFQIKIN
                     DANKIHNLNKNGSSGFYPV"
     gene            complement(952..1224)
                     /locus_tag="ACHJZ2_004532"
     CDS             complement(952..1224)
                     /locus_tag="ACHJZ2_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO4570511.1"
                     /translation="MALKCPECGTTAHARTSAYEAPSVKRSWYQCQNLECSYTFTALE
                     SVDTIIMKPRRNEQESEKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            complement(1230..1781)
                     /locus_tag="ACHJZ2_004533"
     CDS             complement(1230..1781)
                     /locus_tag="ACHJZ2_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO4570512.1"
                     /translation="MMARVTPDQALISFRNARILWAGHSESRKAVEQQIESLLTAMEK
                     PADYARQLELQREHLDVLKWQINCAARECIYSQHQLMEACTEDALSNFMQANGAALTS
                     ALAPFLKGRGGVDVASRILRNALVRQLAITPPEIAGDYRMILDESGVMPDPMMVRDCQ
                     SSYTPAQQLRFQQRLDYINGMQE"
     gene            complement(1778..2530)
                     /locus_tag="ACHJZ2_004534"
     CDS             complement(1778..2530)
                     /locus_tag="ACHJZ2_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFO4570513.1"
                     /translation="MTIPEQNNNPIVEISIPVSTQQALEKVNATKSAWLEARRQQKAA
                     ADNIATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFD
                     ELLAAQESENALLPQEAAELAGKYIHAHDTLVGIRAKQIWEDFMQSHGKALIQTLSLL
                     KSTMGREASAVVGVVHSVNDPDTLLKDFIHKHITRPALTNDAMPEQDPVFKLAGVAPD
                     YAARLDFSNKLSPAAMHKMKVRQERAEKEKAV"
     gene            3464..3724
                     /locus_tag="ACHJZ2_004535"
     CDS             3464..3724
                     /locus_tag="ACHJZ2_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4570514.1"
                     /translation="MRNISVSAPAPAAPLFPVSNHHERFLRLPEVMHLCGLSRSTVYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
     gene            3721..4278
                     /locus_tag="ACHJZ2_004536"
     CDS             3721..4278
                     /locus_tag="ACHJZ2_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4570515.1"
                     /translation="MMIPAYIKYLYSGLLTVVISRYSFSAVAKSAAGIGVPYNLLATI
                     DAPCVFFYVVAQAQPFSGLWCLCLHHGSIEIMVVRAGQPSGWPVSNKAGYANPVRAAT
                     SEIGVSGGSNNRYLLEAAIMATILTPSHPQYVFVFAAIRRADTHPRICMLRTVSCDER
                     SARRLLVRDYVLSLSARLPAGEVTL"
     gene            4275..4496
                     /locus_tag="ACHJZ2_004537"
     CDS             4275..4496
                     /locus_tag="ACHJZ2_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570516.1"
                     /translation="MNQFEISYDDVVRLKHLRNVGEYVTGMAALQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCHDDLMNAEVA"
     gene            4496..4819
                     /locus_tag="ACHJZ2_004538"
     CDS             4496..4819
                     /locus_tag="ACHJZ2_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFO4570517.1"
                     /translation="MICCESLLALRAALYRRAVACAWLALSNHQERYSGLTLAELEDA
                     IARELEGFYLRQHGQQRGLEIACALLSDLMESGPLKACPVLSLLGMTVMDELCSRHLN
                     KPALH"
     gene            4833..7166
                     /locus_tag="ACHJZ2_004539"
     CDS             4833..7166
                     /locus_tag="ACHJZ2_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO4570518.1"
                     /translation="MSGMKVSQAEKAARGHWSRILPALGVNVLKNRHQPCPVCAGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVSKVLDVGISEAADRINGIIGNLLPVSQGMLES
                     GSPEKEDGRKAAAVLAARLFDKSRQTTGNAYLTSKGFPALPCRELTAMHKVGGVAFRA
                     GDLVVPLYADGELVNLQLINANGGKCFLKGGQVKNAFYLVEGTAKAAKRLWIAEGYAT
                     ALTINYLTGDAVMVAFSSVNFLSLASIACSEYPTHQIIIAADRDLNGAGQTRGAAVTG
                     ACNCTMALPPVFGDWNDAFTQNGEEATRHAIHEVIKPAVASPFDTMSEAEFTALSVSE
                     KAQRVVDHYKNSLAVDPNGQLLSRYEAGAWKVIYYADFARDVAALFQRLDAPFSSAKI
                     ASLVETLKLIVPQQQNPARQLIGFRNGVLDTRTGLFSPHDKKHWLRTLCEVDYTQPVD
                     GESLETHAPAFWRWLDRAAGFNPEKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIEMLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPDQIAPEERD
                     TQLKEKIASELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADSAFDFCGYL
                     EVLPDTTGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPLMLKEY
                     GLQYEKRRTNQGMQTNLALREESNADWLPKCDEFAAK"
     gene            complement(7299..>7987)
                     /locus_tag="ACHJZ2_004540"
     CDS             complement(7299..>7987)
                     /locus_tag="ACHJZ2_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570519.1"
                     /translation="FVEFNDTIYAFLKGYYHNQLSLNRIYNTKCSSCSFENSGSYMVD
                     VYRTVKRVNETIQTPNINCYTTCILTRPANGHVTSTGFSRNLMDAINISLAEHKIASL
                     HNEVMSIEEYDRDSLQEKGNTARRYLEYILMLVNIRIMHLNNVQYQEQMLGSLVSVIE
                     ALDYEPLMKNDVEITKDILNACSHHGGVRIEKKDVIFSLEVIENLIKAIKKTDINKLQ
                     LDGMFKSIQK"
BASE COUNT         1987 a         1830 c         1927 g         2243 t
ORIGIN      
        1 tatcttcatt acaagataaa gaagatgatg ttgacgaact taaatactcc gccgaggact
       61 ttgactcctt aacagtagct gatttgtatg atatagaaat agcgatgcaa gactttttga
      121 atgatataaa ttttgaaaat tctaaagata acaaagtgcg atttgacgaa gatacatatg
      181 actttaatat caatgggaaa cgccgaggaa tgtttggtaa aggtacccgt gcagtaatgc
      241 atgctatatt taccatttgc tttgcggaat ttttatcaag aaaagggaat ccattcattg
      301 gatttgttgt gcttgatagt ccattagtga cgcactttga taaagataga gggggttctc
      361 tatcggatgt taatagcgtc agtctcagtg attcatttta tcacgcatta atcaaaaggg
      421 actataattt ccagattgta attcttgaga ataaaggtcc gacgttccaa ataaaaatta
      481 atgatgcaaa taaaatccat aacttaaata agaatggaag ctctggtttt tatcctgttt
      541 aatacattaa aaatataact gctaattggg gtgctcgaaa ttatatttcg tttcgagcgc
      601 tcagttcatt tctaaataat ttgcaaaatt attttgggta ctttcgcctc tacacttcct
      661 tcgggtggtt ccacaataat ggatctcacc ggcgtttgat attttataat ctaacccctt
      721 agcgcgcgca gccccccacg cctgctcgct tcgcttaaca gactggtttt catgcattcc
      781 gcaaatcgtc tcagaagcca ccacacaagg gctttcgcgt caaaaatggt gcatgagaat
      841 catgcgtttt catgcgctat agacatgcac tcatgcgctc tcaggccagc cagggaaaag
      901 gcgtaaaaaa tcccagtact ggaccgggat tacgtgggct ttttttgcta atcagacagg
      961 aatttgctga cggcttgaca gctttgacgc ggagccatag cgattgagag tttgctgttg
     1021 tttttccggc atttgtgctt tctctgattc ctgttcattg cgccggggct tcatgattat
     1081 cgtatccaca ctttccagtg cggtaaatgt gtaggaacat tcaagattct ggcactgata
     1141 ccatgagcgt ttaaccgatg gtgcttcata ggcggaggtt ctggcgtgtg ccgtcgtacc
     1201 gcattcggga catttaagtg ccattaatat tactcctgca tgccgttaat gtaatccaga
     1261 cgttgctgaa agcgcagttg ttgtgcaggg gtgtaagagc tctggcaatc acgaaccatc
     1321 atggggtcag gcatcacacc ggattcatcc agaatcatac gataatcccc ggcaatttca
     1381 ggcggtgtta ttgccagctg gcgaaccaga gcattgcgta atatacggga cgcaacgtca
     1441 acccctccgc gccccttcag gaacggagcc agtgcagatg tcagggctgc tccattcgcc
     1501 tgcataaaat tgctgagagc gtcttctgtg caggcctcca tcagttgatg ctgcgagtaa
     1561 atgcattcgc gcgcagcaca gttaatttgc catttcagca catccagatg ttcccgctgt
     1621 aattcgagct gtcgagcata atctgctggt ttttccatgg cagtgaggag tgattctatc
     1681 tgttgttcaa cggcttttcg gctctcacta tgtccagccc acagaatacg ggcatttcta
     1741 aaactgataa gcgcttggtc tggagtgact cgggccatca tactgctttc tccttctcag
     1801 cacgttcctg acgaaccttc attttatgca tagctgctgg agagagttta ttactgaaat
     1861 ccagacgggc cgcataatcc ggggcaactc ccgccagttt aaacacgggg tcctgttcgg
     1921 gcatcgcatc gttagtcaga gccggtctgg tgatatgttt atgaataaaa tctttcagca
     1981 gtgtgtccgg gtcattaact gaatgcacca caccgacaac tgcgctggct tccctgccca
     2041 ttgttgattt gagcaggctt agtgtctgga ttagtgcttt tccatgcgac tgcataaaat
     2101 cttcccagat ttgttttgcg cgaataccca caagggtgtc gtgcgcgtgg atatactttc
     2161 cggctaattc cgcagcctcc tgcggcaaaa gtgcattttc gctttcctga gccgccagca
     2221 gctcatcaaa atcctcaagc gtttctcgtc ccagcgcaat ttccgtgcgc agttttttca
     2281 tttcctttga gactacgccc tgactttcac gaaatagcgt gcgccactcc tcattcaggg
     2341 cgttcgtcgt ggcttccatt tcagcgcggc gctggcggat tgtcgcaata ttatccgctg
     2401 ccgctttttg ctgacgacgt gcttcaagcc atgcagattt ggtggcattc actttctcaa
     2461 gcgcctgttg cgtcgaaaca gggatagaaa tttcaacgat cgggttattg ttctgttctg
     2521 ggattgtcat gtcagcacct agtggtttgt cgtggtgtca attgtgctgt gatgcataca
     2581 aagctgccat tgaacgccat tgtgcgagcg acaagacaaa gactgaaccg ccccggacct
     2641 cttttggcta gccagaaaag gcctcgttta actaaggctg tttcagactt accttaacta
     2701 ttcactacta ttcacttagg taaaaaaaat aggtaataca gaaggttata aggtgaaggg
     2761 ttggcatggc agtattcatc gactgttcat aactgttcac atgggaataa aattatttat
     2821 ggtatttttt cataatgggt ggttttttta ttcctcgtag ctatttgttt tttgctcctc
     2881 accactattc acccttattc aggagtattc aacaggcaca taaatagact gcatgttttt
     2941 gccagggaat aacattttcg cctctggcca tggtgttgca tttcatactg atttttataa
     3001 aactagatct gtccggttct ttatggattc attcggatat attttacgat ttaaggtgat
     3061 gttatttaat atcaaggttt ttatgcaggc ccaatgttta taagttggat gcgattcaaa
     3121 aatatgcccg gaaattgggg cctgcaatgc aaggcctcgt gtagaagttg tactgccttg
     3181 ctgtattggc ggtggggtta gcactatcag gtgcaatgac ccataggata gtaaaccctg
     3241 ggtgaatggt agttcctatt aatgctcctg tatttatagt ccgcaggcat ggtatttatt
     3301 atgtcatttt atatggttta atcgggcttt aaattccata aaatcaatat gtgacgctaa
     3361 ataaaaaatg cgtgcgcagg tggtttatat acacataata aggaactacc tgaacccgga
     3421 taaattcatc cggactgttg tggaaattaa agagggtaga taaatgcgta atatttctgt
     3481 gtctgcgcca gctcctgctg cgccattatt tcctgtatcg aaccatcatg agcgattttt
     3541 acgcctgcct gaggtgatgc acttatgcgg gttgtcccgt tcgaccgttt acgacctgat
     3601 cagccgcgat gcgtttccga agcagatccc gcttggcggt aaaaatgtcg cctgggcaca
     3661 gtctgaggtc agcgcatgga tggcggaccg catcagcgcc cgtgggcggg gatgtgatgc
     3721 atgatgatac ctgcatacat taaatacctt tattctggct tgcttactgt cgtcatttcc
     3781 aggtatagtt tttccgctgt cgcaaaatcg gcagccggaa ttggcgttcc gtataactta
     3841 ttggcgacaa ttgacgcccc ttgcgtcttt ttttacgtcg tagctcaggc acaaccattt
     3901 tcagggctgt ggtgtttatg cttacaccat ggttctatcg agataatggt agtccgggcg
     3961 gggcagcctt cgggctggcc ggtttccaat aaggccggtt acgccaaccc cgttcgggct
     4021 gccaccagtg aaattggcgt ttccggtggt agcaataacc gctacttatt ggaggctgcc
     4081 atcatggcta caatcctcac cccgtcacac ccgcaatatg tctttgtgtt tgccgcaatc
     4141 cgccgtgctg acactcaccc ccgcatctgt atgcttcgca ctgtctcctg cgatgagcgt
     4201 tccgcgcgcc gtttgctagt ccgagattat gtactctccc tttctgcacg gctgcctgcc
     4261 ggggaggtga cgctatgaac cagtttgaga tttcctacga cgatgttgta aggctgaaac
     4321 atttacgcaa tgtgggtgag tatgtaactg gcatggcagc cctgcaggac tgttatgaaa
     4381 agccagcagg tgcccaatgc gaacaactgg tttccctcat ttatctgatg acagagcaac
     4441 tggatggagt ggtgcaacgc tgccatgacg acctgatgaa tgcggaggtg gcctgatgat
     4501 atgctgtgaa tctcttctgg cacttcgtgc cgctctgtat cgccgtgccg ttgcctgtgc
     4561 ctggctggct ctaagcaatc atcaggaacg ttattccggc ctgacgctcg ctgaacttga
     4621 agatgccata gcccgcgagc tggaagggtt ctatctgcgc cagcatgggc agcaaagagg
     4681 gctggaaatt gcctgcgcgt tgctttcgga tctgatggag tcggggccgc ttaaagcctg
     4741 tccggttctc tcactgctcg gaatgacggt catggatgaa ctttgttccc gtcacctcaa
     4801 caaaccagcg ctgcactaag gagggccgca caatgtcagg aatgaaagtt agccaggctg
     4861 agaaagcagc tcggggtcac tggtcaagaa ttttacctgc gctgggcgta aatgtactga
     4921 aaaatcggca ccagccctgc ccggtctgtg ccgggaaaga ccgctttcga tttgatgacc
     4981 aggaagggcg gggaacgtgg ttctgtaacc agtgcggggc aggtgatggc ctggcgcttg
     5041 taagtaaagt actggatgta ggcattagtg aagcggcaga cagaataaac ggcattatcg
     5101 gaaacctgct gccagtatct cagggaatgc ttgaatctgg ttctcctgaa aaagaggacg
     5161 ggagaaaagc tgcagcagtg ctggctgccc gtttgtttga taagtcccgc cagaccactg
     5221 gcaatgccta tctgacgagt aaagggtttc ctgcactgcc ttgccgggaa ttaaccgcta
     5281 tgcataaagt cggtggtgtg gcatttcgcg cgggagatct tgtcgttcca ttgtatgcag
     5341 atggagagct ggtaaatctg cagttaatca acgctaatgg gggcaaatgc ttccttaaag
     5401 gcggtcaggt taagaatgcc ttttacctgg ttgaaggtac tgccaaagca gccaaacggc
     5461 tctggatagc ggaaggatat gccaccgcac ttactatcaa ctatctgact ggcgatgctg
     5521 tcatggtggc cttttcgtcc gtcaatttcc tttccctggc gagcattgcc tgcagtgagt
     5581 acccaacgca ccagataatt attgctgctg accgcgatct caacggtgcg gggcaaacaa
     5641 ggggcgcagc tgttaccggg gcctgcaatt gcacaatggc gctcccgcct gtgtttggtg
     5701 actggaacga tgcattcacg caaaacggcg aagaagccac ccggcatgca attcatgaag
     5761 taataaaacc agctgttgcc agccccttcg acacaatgag cgaagctgaa tttaccgcgc
     5821 tgagcgtcag cgaaaaagcg cagagggtag tggatcacta taaaaattca ctggcagtag
     5881 acccgaacgg gcagctcctt tcacgctatg aggcgggggc ctggaaagtt atctattacg
     5941 ccgattttgc ccgtgatgtc gctgcgctgt ttcagcgcct cgacgcacct ttttcatccg
     6001 cgaaaattgc gtctctcgtg gaaaccctca aactgatcgt tccgcaacag cagaatccgg
     6061 cgcggcaact tatcggattt cgcaacggtg tgctcgatac ccggacagga ttgttcagcc
     6121 cgcacgataa gaagcactgg ttacgtacgc tgtgcgaggt ggattacacg cagcccgttg
     6181 acggtgagtc actggaaacc catgccccgg cattctggcg ctggctggat cgtgccgcag
     6241 gttttaatcc tgaaaaacgg gacattattc tggctgcatt gtttatggtg ctggctaacc
     6301 gttatgactg gcagctgttt ctggaggtca ctggccctgg cggaagtgga aagagtattc
     6361 ttgctgaaat agcaaccatg ctggcgggtg aagataacgc gacctcggca accattgaaa
     6421 tgcttgagtc gccaagagaa cgagctgcgt taataggttt ttcactgatt cgacttcccg
     6481 accaggaaaa gtggagcggt gacggggccg gactaaaagc catcactggc ggcgatgcgg
     6541 tatccgttga tcccaaatat cagaacgcct attcaaccca catcccggcg gtcatcctgg
     6601 ctgtgaacaa taatccgatg cgcttcactg atcgtagtgg tggagtttca cgccgaaggg
     6661 tgatcctgca tttccccgac cagatagccc cggaggaacg cgatacccag ctcaaagaga
     6721 aaattgccag tgagctagcg gtgattgttc gccagcttat gcagcgtttc agcgacccaa
     6781 tgagtgccag gacattgctt cagtcacagc agaactccga tgaagcgctc accatcaaac
     6841 gtgatgctga ttcagctttt gatttttgcg gctaccttga ggtcctacct gacaccacgg
     6901 gcatgtttat ggggaacgct aatattgttc cacgtcagcc tcgaacttac ctctaccatg
     6961 cctatctggt ctacatggag gctaacggct ataaaaatac gctcagtctg accatgtttg
     7021 gcaaggggct accgttaatg ctcaaggaat atgggctgca gtatgagaaa cgacggacca
     7081 atcaaggaat gcagactaat ctggccctaa gagaggaaag caatgctgac tggttgccaa
     7141 aatgcgatga gtttgcagcg aaataactga cctaagaccg gctgtgccgg tcttaaatcc
     7201 gctacaggaa attatctcaa aatatatatc gataaaattt aaaagttata tgctaaggcg
     7261 tcagcggaaa atgcatctaa ttcattgtta gatgatgttt atttttgaat tgatttaaac
     7321 atcccatcga gttgtaattt atttatgtcg gttttcttta tggctttaat taagttttca
     7381 ataacttcca aagaaaaaat tacatctttc ttttcaattc taaccccccc gtggtggctg
     7441 caagcattaa ggatgtcctt tgtaatttca acatcatttt tcattaatgg ttcatagtcc
     7501 agagcctcaa taacagaaac taaactacct agcatttgct cttggtattg aacgttgttt
     7561 aaatgcatta tacgaatatt aacaagcatg agaatatatt ctaaatatct cctggcagta
     7621 tttcctttct cttgtaaaga atcacgatca tattcctcaa tactcatgac ttcattatgt
     7681 aaagaggcta ttttgtgctc tgctaatgaa atatttatcg catccatgag gtttcgtgag
     7741 aagcctgtag atgtaacatg accatttgcg ggccttgtta aaatacatgt agtatagcaa
     7801 tttatatttg gggtctggat tgtctcattt actcttttaa ccgttctata aacgtctacc
     7861 atataagaac ctgagttttc aaatgaacat gaagagcatt ttgtattata tattctattt
     7921 agagataact gattgtgata gtatcccttt aagaaagcat atatagtgtc attaaactcg
     7981 acaaaca
//
LOCUS       DAZFBR010000173         7972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFBR010000173 DAZFBR010000000
VERSION     DAZFBR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.173"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            135..224
                     /gene="kdpF"
                     /locus_tag="ACHJZ2_004541"
     CDS             135..224
                     /gene="kdpF"
                     /locus_tag="ACHJZ2_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO4570520.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            224..1897
                     /gene="kdpA"
                     /locus_tag="ACHJZ2_004542"
     CDS             224..1897
                     /gene="kdpA"
                     /locus_tag="ACHJZ2_004542"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO4570521.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1920..3968
                     /gene="kdpB"
                     /locus_tag="ACHJZ2_004543"
     CDS             1920..3968
                     /gene="kdpB"
                     /locus_tag="ACHJZ2_004543"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO4570522.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3977..4549
                     /gene="kdpC"
                     /locus_tag="ACHJZ2_004544"
     CDS             3977..4549
                     /gene="kdpC"
                     /locus_tag="ACHJZ2_004544"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO4570523.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4542..7226
                     /gene="kdpD"
                     /locus_tag="ACHJZ2_004545"
     CDS             4542..7226
                     /gene="kdpD"
                     /locus_tag="ACHJZ2_004545"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO4570524.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7223..7900
                     /gene="kdpE"
                     /locus_tag="ACHJZ2_004546"
     CDS             7223..7900
                     /gene="kdpE"
                     /locus_tag="ACHJZ2_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO4570525.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFITETG
                     IGYRFML"
BASE COUNT         1642 a         2083 c         2359 g         1888 t
ORIGIN      
        1 tactaaacat tagttaaatc atggcttttg ccatttttat acttttttta caccccgtcc
       61 gcagattttt gcgaaatctt tgcagccaga attctaccct tccggtatca cttttaggcc
      121 actggaggtg cactgtgagt gcaggcgtga tagccggcgt gttgctggtg tttttattac
      181 tgggttattt ggtttatgcc ctgatccatg cggaggcatt ctgatggctg cgcaagggtt
      241 cttactgatt gccacgtttt tactggtatt aatggtgctg gcgcgtccct taggtagcgg
      301 gctagcgtgg ctgattaatg atattcctct tcctggtacg acgggcgttg aacgcgtact
      361 ctttagcgcg cttggcgtct ctaaccatga gatgaactgg aagcagtatc tctgtgccat
      421 tctcggcctg aacatgctgg ggctggcggt gctgtttttt atgttgctcg gtcagcacta
      481 tctgccgctt aatccacagc agttgccggg gctgtcgtgg gatctggcgc tgaataccgc
      541 cgtcagcttt gtcacgaata ccaactggca atcttatagc ggtgaaacca cgttgagcta
      601 tttcagccag atggcgggct taacggtgca aaactttctt tctgccgcca gcgggattgc
      661 ggtgattttt gccctcattc gtgcatttac ccgccagagc atgagcacac tcgggaatgc
      721 ctgggtcgat ctgctacgca tcacgttatg ggtgctcgtc cctgtggcgt tggtgatcgc
      781 actgtttttt attcaacaag gtgcgctgca aaactttctg ccctatcagg cagtgaatac
      841 cgttgaagga gcgcaacagc tgttaccaat gggtccggtg gcttctcagg aagcgatcaa
      901 gatgctcggc actaacggcg gtggtttctt taatgccaac tcgtcgcatc cgtttgaaaa
      961 cccaaccgca ctgaccaact tcgtgcagat gctggcgatc ttcttgatcc caacagcgct
     1021 gtgctttgcc tttggtgaag tgacgggcga tcgccgtcag gggcgcatgt tgctgtgggc
     1081 gatgtcagtg atttttgtca tctgcgtagg cgtggtgatg tgggcagaag ttcagggtaa
     1141 tcctcatctg ctggcgctcg gcgcagacag cagcatcaat atggaaggta aagagagccg
     1201 tttcggcgtg ctggtcagta gcctgtttgc ggtcgtgact acggcggcgt cctgtggcgc
     1261 ggtgattgca atgcatgatt cgtttaccgc tctcggtggc atggtgccga tgtggctgat
     1321 gcaaattggt gaagtggtat tcggcggtgt cggttctggt ctttacggca tgatgctgtt
     1381 tgtcctgctg gcggtgttta ttgccgggct gatgattggt cgtacaccgg aatatctggg
     1441 taaaaaaatc gacgtacgtg agatgaaact gactgcactg gcgattctgg tcaccccgac
     1501 gctggtgctg atgggcgcgg cgctggcaat gatgaccgac gccggacgta gcgccatgct
     1561 caaccctggc ccgcatggtt ttagcgaagt gctgtacgcc gtgtcatccg ccgctaacaa
     1621 caacggcagc gcctttgccg gattaagcgc caactctccg ttctggaact gtttactggc
     1681 gttctgcatg tttgtcggtc gcttcggggt gattatcccg gtgatggcaa ttgccggttc
     1741 gctggtgagt aaaaagagcc aacccgccag ctccggcacg ctgccaacgc acggcccgct
     1801 gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc gcactgacct ttatccctgc
     1861 cctggcgctt ggtccggtgg cggaatatct ctcctgatga tattgagtga gcactgaata
     1921 tgagtcgtaa acaactggcg ctattcgaac caacacttgt cgttcaggcg ctgaaagaag
     1981 cggtgaaaaa attaaacccg caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga
     2041 tcggcagtct gctgaccacc tgtattagca tcgcgatggc aagcggcgcg atgcctggca
     2101 atgcgctgtt tagcgtggcc attagcggct ggctgtgggt caccgtactg ttcgctaatt
     2161 tcgccgaggc gctggcagaa ggccgcagta aagcgcaggc caacagcctg aaaggggtga
     2221 aaaaaacggc ctttgcccgc aagctgcgtg agccgaaata tggcgctgcg gcggacaaag
     2281 ttcccgccga tcaactgcgt aaaggcgata tcgtactggt agaagcaggc gatattatcc
     2341 cctgcgatgg tgaagttatt gaaggcggcg catcggtcga tgaaagcgcc atcaccgggg
     2401 aatcggcacc ggtgatccgt gaatccggcg gcgattttgc ctccgtcacc ggcggcacgc
     2461 gtattctttc tgactggctg gtgattgagt gtagcgttaa ccccggcgag acatttctgg
     2521 atcggatgat cgcgatggtg gaaggcgcac agcgacgcaa aacgccgaac gagattgccc
     2581 tgaccatcct gctgattgcc ctgactatcg tctttttact ggcaaccgcc acgctgtggc
     2641 cgttttccgc gtggggcggt aatgcagtca gcgtaacggt actggtggcg ctgctggtct
     2701 gtctgatccc aaccactatt ggcggcctgt tgtcagcgat cggcgtcgcc gggatgagtc
     2761 ggatgctggg cgcgaatgtg gttgccacca gcggacgtgc agttgaagcg gcaggtgacg
     2821 ttgacgttct gctgctggat aaaaccggca ccatcacact cggtaaccgt caggcatcgg
     2881 agtttatccc tgcgcagggc gtggatgaaa aaacgctggc tgacgccgca caactggctt
     2941 cgctggctga tgaaacgccg gaaggccgca gtattgtgat cctcgccaag cagcgtttta
     3001 acctgcgcga gcgcgatgtg cagtcgctcc atgccacctt tgtaccgttt actgcgcaaa
     3061 gccggatgag cgggatcaat atcgacaacc gcatgatccg taaaggttct gtcgatgcca
     3121 ttcgccgcca tgttgaggct aacggtggtc acttccctgc cgatgttgat caaaaagtcg
     3181 atcaggttgc gcgtcagggg gccacaccgc tggtagtagt agaaggttct cgtgtgctgg
     3241 gcgttattgc gttgaaagat atcgtcaaag gcggcattaa agaacgcttc gcccaactgc
     3301 gcaaaatggg tattaaaacg gtgatgatta ccggcgataa ccgtctgact gccgccgcga
     3361 tagctgcgga agcgggcgtc gatgattttc tcgccgaagc gacaccggag gccaagctgg
     3421 cattgatccg tcagtatcag gcggaaggcc gtttggtagc aatgaccggc gacggcacca
     3481 acgatgctcc ggcgctggcg caggcagatg tcgcggtggc gatgaactcc ggtactcagg
     3541 cggcgaaaga agcgggcaat atggtggatc tcgactctaa cccgaccaag ttgatcgagg
     3601 tggtacatat tggcaaacag atgctgatga cccgtggctc gctgaccacc ttcagcattg
     3661 ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc attcgcggta acgtatccac
     3721 agttaaatgc gctgaacatc atgcgcctgc attcgcccga ctccgccatt ctcagtgcgg
     3781 tcattttcaa cgccttgatt atcgtctttt tgattcccct ggcgttaaaa ggcgtgagtt
     3841 ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac ggtctgggtg
     3901 ggctgctggt gccgtttatt ggtatcaaag tcattgattt actactgacc gtttgcggtc
     3961 tggtgtgagg tttaccatgc gtggattacg tccggcatta tcgacattta tctttctgtt
     4021 attgattact ggcggtgttt acccgctgct gaccaccgcg ctggggcaat ggtggtttcc
     4081 ctggcaggcc aacggttcgt tgatccgtga aggtgatacg gtgcggggtt cggcattaat
     4141 cgggcagaat tttaccgaca acggctattt tcatggtcgc ccgtcggcaa cggcagaaat
     4201 gccttataat ccacaggctt ctggcgggag caatctggcg gtcagtaacc ctgagctgga
     4261 taaactaata gccgcacgcg ttgctgcatt acgggccgct aacccgaatg ccagcacgag
     4321 cgttccggtt gaactggtga cggcgtcggc aagcgggctg gacaataaca tcaccccgca
     4381 agcggcggcc tggcagatcc cacgcgtagc gaaagcgcgt aatctcagcg ttgaacagct
     4441 cacgcaactg atcgcaaaat acagccaaca accactggtg aaatatatcg gccagccggt
     4501 cgtcaacatt gtcgaactca atctggcact ggataaactt gatgaataac gaacccttac
     4561 gtcccgaccc cgatcgtctg ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag
     4621 ttttcttcgg tgcctgcgca ggcgtcggga agacctgggc gatgctggca gaagcccagc
     4681 gactgcgggc gcaagggctg gatattgtgg ttggcgtggt agaaacccac gggcggaaag
     4741 ataccgccgc catgctggaa gggctggctg ttctgccgcc aaaacgcctg gcgtaccgtg
     4801 ggcggcacat cagcgagttt gatctcgatg ccgccctcgc ccgccgtccg gcgctgatct
     4861 taatggacga actggcgcac agtaatgcgc caggttctcg tcatcctaaa cgctggcagg
     4921 atatcgaaga actgctggaa gctggcattg atgttttcac aaccgtcaac gttcagcatc
     4981 tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc
     5041 ccgatccttt tttcgatgcc gccgacgacg tggtgctggt ggacttgccc ccggacgatc
     5101 tgcgccagcg gctgaaagaa ggcaaagtct atattgccgg gcaggcggag cgcgccattg
     5161 aacatttttt ccgcaaaggc aatctgatcg ccctgcgcaa actggcactg cgccgtactg
     5221 ccgatcgcgt tgatgagcaa atgcgcgcct ggcgggggca tcctggcgaa gagaaagtct
     5281 ggcacacgcg cgacgcgatc cttttatgca tcggccataa caccggcagc gaaaaactgg
     5341 tccgcgcggc ggcgcggctg gcgtcacggc tggggagcgt ctggcacgcg gtgtatgttg
     5401 aaacccctgc cctgcatcgc ttaccggaga aaaaacgtcg ggcaattctc agcgccttac
     5461 gtctggcgca ggagctgggc gcggagacgg caacactttc tgatccagcg gaagagaaag
     5521 cggtagtgcg ttatgcccgt gaacataatc tcggcaagat tattctcggt cgcccagcct
     5581 cgcgccgctg gtggcgtcgg gaaacgtttg ctgaccgact ggcgcgcatc gcccccgatc
     5641 tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg cacgattaac aacgcgccgg
     5701 atagccgctc ttttaaagac aagtggcggg tacagattca gggatgcgtg gttgccgccg
     5761 cgttatgcgc cgttatcacc ttaattgcca tgcagtggct gatggcgttt gatgccgcca
     5821 acctggtgat gctgtatcta cttggcgtgg tggtggtggc gctattttat ggacgctggc
     5881 cttcggtggt tgctaccgtc attaatgtgg tgagtttcga tctctttttt atcgccccac
     5941 gcggcacgct cgccgtctct gatgtgcaat atctgctgac cttcgcggtg atgttaaccg
     6001 tcgggctggt gatcgggaac cttactgcgg gtgtgcgtta tcaggcgcgg gtagcccgtt
     6061 accgcgagca acgcacacgg cacttatatg aaatgtcgaa agctctggcg gtgggccgca
     6121 gtccgcagga catcgccgcc accagcgaac aatttattgc ctccacgttt catgcccgca
     6181 gtcaggtgtt gttgcccgat gacaacggta aattgcagcc gttaacacat ccgcaaggaa
     6241 tgacgccgtg ggacgatgcc atcgcgcagt ggagttttga taaaggcctg cctgcgggtg
     6301 cgggcaccga cacgttaccc ggcgtgccgt accagatttt gccgttaaaa agcggtgaga
     6361 aaacctacgg gctggtggtg gtggaaccgg ggaatctgcg tcagttgatg atcccggaac
     6421 agcagcgcct gctggagacg tttacgctgt tagtcgccaa tgcgcttgag cggctgacgc
     6481 tgaccgccag cgaagaacag gcgcggatgg caagcgaacg tgaacagatc cgcaacgccc
     6541 tgctggcggc gctttcgcat gatttacgca ctccgcttac ggtgctgttt ggtcaggcag
     6601 aaatcttaac gctcgatctg gcaagcgaag gatcacccca cgcccgccag gccagcgaga
     6661 ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat atggcgcgaa
     6721 ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa gtagtcggca
     6781 gcgcgctgca aatgctggaa ccgggtttat cgtcgcccat caatctttct ctgccagaac
     6841 cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt aatctgctgg
     6901 agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc cacgttgagg
     6961 gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcca ggccaggagc
     7021 agacgatatt tgataagttt gctcgcggga ataaagagtc ggcagtaccg ggggtagggc
     7081 ttggactggc aatttgtcgg gcgatagtgg atgtacacgg gggcactatt accgcgttca
     7141 accggccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaact gcccctgaac
     7201 ttgaagaatt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg aacaggctat
     7261 tcgtcgcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctatg aggccgaaac
     7321 gctgcaacgc ggcttgctgg aagcggctac ccgtaagcca gatttgatta ttctcgatct
     7381 cggcctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt ggagcgcggt
     7441 gccggtgatt gtgctttccg cacgcagcga agagagcgac aaaatcgccg cgctggatgc
     7501 cggagcggat gattatctaa gtaagccgtt tggcattggc gaattgcagg cccgtctgcg
     7561 cgtcgcttta cgccgccact ccgccaccgc cgcgcccgat ccgctggtga aattttccga
     7621 tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg tgcatctcac
     7681 cccgattgag tttcgcctgc tggcagtgct gctcaacaac gccggaaaag tgctcaccca
     7741 gcgccaactc cttaaccagg tgtgggggcc aaacgcggtc gaacacagtc attatttgcg
     7801 tatttatatg ggacatctgc gacaaaaact ggaacaggat cccgctcgcc ccagccattt
     7861 cattactgaa accggtattg gttatcggtt tatgctttga atttttattc aataaattcg
     7921 cctaaatagt gattaattta ttattaaacc tgcgccaaat attattattt gg
//
LOCUS       DAZFBR010000174         7959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFBR010000174 DAZFBR010000000
VERSION     DAZFBR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.174"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            41..580
                     /gene="elfA"
                     /locus_tag="ACHJZ2_004547"
     CDS             41..580
                     /gene="elfA"
                     /locus_tag="ACHJZ2_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4570526.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /gene="elfD"
                     /locus_tag="ACHJZ2_004548"
     CDS             663..1364
                     /gene="elfD"
                     /locus_tag="ACHJZ2_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4570527.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /gene="elfC"
                     /locus_tag="ACHJZ2_004549"
                     /pseudo
     CDS             1389..3988
                     /gene="elfC"
                     /locus_tag="ACHJZ2_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /gene="elfG"
                     /locus_tag="ACHJZ2_004550"
     CDS             3979..5049
                     /gene="elfG"
                     /locus_tag="ACHJZ2_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4570528.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTTGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5077..5604
                     /gene="ycbU"
                     /locus_tag="ACHJZ2_004551"
     CDS             5077..5604
                     /gene="ycbU"
                     /locus_tag="ACHJZ2_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4570529.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            5612..6127
                     /gene="ycbV"
                     /locus_tag="ACHJZ2_004552"
     CDS             5612..6127
                     /gene="ycbV"
                     /locus_tag="ACHJZ2_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4570530.1"
                     /translation="MLKRIIWILFLLGLTGGGELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6093..6829
                     /gene="ycbF"
                     /locus_tag="ACHJZ2_004553"
                     /pseudo
     CDS             6093..6829
                     /gene="ycbF"
                     /locus_tag="ACHJZ2_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHJZ2_004554"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHJZ2_004554"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO4570531.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
BASE COUNT         2159 a         1748 c         2011 g         2041 t
ORIGIN      
        1 aaggcattat catatttacg aagagatcag gatgataacg atgaaaaaaa gtgtattgac
       61 ggcgtttata actgtggtat gtgcaacgtc cagcgttatg gctgctgatg ataatgctat
      121 cacggatggc tcagtaacat ttaatggtaa agttattgct ccagcttgta ccctggtagc
      181 tgcgacgaaa gattccgtgg tgactttgcc agatgttagt gccacgaagt tgcaaaccaa
      241 tggtcaggtt tctggcgtgc aaactgatgt gccaattgaa ttaaaagatt gtgatactac
      301 cgtaacaaaa aatgcaacgt tcacctttaa tggcactgcg gatactactc agattacagc
      361 gtttgctaac caggcctcat ctgatgctgc tacaaacgtg gccctgcaaa tgtatatgaa
      421 tgatggtaca acggccatca agccagacac agaaaccggg aacattttgt tgcaagatgg
      481 agatcagacg ttgactttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctccag tgttatggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgttcgtc cacatgggcg gcaggtaaag gcggggttgg acttgcagca acacgtcttg
      781 tttattcgga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatctgat tcagtcatgg gtgatgaccc cagataataa aaaatcagcg gactttatta
      901 ttacaccacc gctatttgtg ctgaatccgg caaacgagaa tctgttacgc attatgtaca
      961 ttggcgcgcc gttggcgaaa gacagagaaa ctcttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gaaggcaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggt ggaaagttag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggtgcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgactacgg ggcggaaacg ccgcaactca actgtaaatc gtaattcgtc ttcagttaag
     1381 agagcgagat gtatagaact caccgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc agcctttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gcgtgaatac tgatgcattt ccggctttta
     1741 aacaactgga caaacaagcg tgtgcgccat tggcggagat tattccggat gccagcgtaa
     1801 cttttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaattgcc atcaaaagta
     1861 atgctcgtgg ttatgtcccc ctgaacgttg ggatgaaggg atcaacgcgc tattactggg
     1921 atattcattt agcggggcta acagtattca tagcagcgca gacagtgatt ctggcgacag
     1981 ttattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc gcaacaattc
     2041 aacatggagt cgtagtagtg gccaaaccgc agaatggaag aatctcagca gctatttgca
     2101 acgggcggtt attcctctga aaggcgaact gaccgtaggt gatgattata ctgcaggcga
     2161 ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca acatgctgcc
     2221 agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca atgcccagat
     2281 aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg gtgcttttga
     2341 aattagtgat atctattcca cgtcgtcgag cggtgatttg ttagttgaaa tcaaagaagc
     2401 ggacggcagc gtcaatagct acagcgtacc cttttccagc gtgccattac tccagcgtca
     2461 ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta atgaacagca
     2521 ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa cgacctggta
     2581 tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg gatttaacct
     2641 cggcgatttc ggagcaattt cgttcgatgt gacccaggcg aagagtacgc tggcagacca
     2701 aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca accaattggg
     2761 tactaacttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca ccctttccga
     2821 caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg aagatacgcc
     2881 gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc aggtcaatat
     2941 ctcccagcaa ttaagcgagt acggttcgtt ttatttaagt ggtagccagc aaacttactg
     3001 gcataccgat caacaggatc ggctattaca gtttggctat aacacgcaaa ttaaagatct
     3061 ctcgctgggg atctcctgga actacagtaa gtcccgtggc caacctgatg ctgatcaggt
     3121 gtttgcactt aatttttccc tgccactcaa tctgttgctc tccagaagta atgatagcta
     3181 taccagcaaa aaaaattacg cctggatgac ctctaacacc agtatcgata acgaagggca
     3241 cactacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggcagcgcca gcatggacta
     3361 caaaggggtg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcactg ggggaaacta acgttctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgttc cttatgccac
     3601 ttcttatcgt gaaaatcgaa tcgcacttga tgcggcgtcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatat cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcataattg atgatgatgg
     3841 ctcgctctat atggcgggtt taccggcgaa gggaacaata accgtgcgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacga gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtc attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ccgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacgactgg ggtagtaaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta cagcggttcc tttcgaaaac
     4381 gtggggactg gggcagatac ccggtggcat atttgtgacc ccggcggtca acgtttaggc
     4441 ggccagggag ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggcaca ccggttttag tgtattacct gtctggtact
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgtatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaaa aaaacgatat ttcagtgcgt tatcttgttc
     5101 tttagcattc ttaacatcca tgtcgggatg gctgggcctg aacaagttag tatgcatatt
     5161 tatgggaatg tggtcgatca gggctgtgat gtcgccacca aaagtgcatt acaaaatatt
     5221 catattggtg attttaatat cagtgatttt caggccgcga ataccgtaag cactgctgct
     5281 gatttgaata tcgatatcac cggttgtgcc gctggtatta ctggcgcgga cgtccttttt
     5341 agcggcgagg ctgacaccct tgcgccgaca ctgctcaaac taactgacac aggcggaagc
     5401 ggtggtatgg caacggggat tgccgtgcaa attcttgatg cgcaaagtca gcaagaaatc
     5461 ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac actcaaatat
     5521 caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac ggcggttctc
     5581 tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct ggattctgtt
     5641 cttactggga ttaacggggg ggggtgagct atttgcccat gatggcacgg tcaacattag
     5701 cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa tcaatgtgca
     5761 gttaggcgat gtctcactga cacgtttttc tcatgggaat tatggcccgg agaaatcttt
     5821 catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgacgtca ccttttcagg
     5881 aacccccgac ggagtgcaga gcgaaatgtt gtccattgaa agtggtacgg atgcagccag
     5941 cgggctggca attgcgattc tggatgatgc gaagatattg attccgctta accaggcgag
     6001 taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc aattgcgacc
     6061 tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg tacttcatta
     6121 tgactaatac gtggaacaga ttggcgctct tgattttcgc cgttttatcg ttgctggtgg
     6181 cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt ccggcagaca
     6241 gagaatcgat atctatttta ctgactaata ccagtcagga atcctggctt attaacagta
     6301 aaatcaaccg cccaacgcgt tgggccgggg gtgaagcgtc gacagtgcca gcaccattac
     6361 tggccgctcc gccacttatt ctcctgaagc ccggtacgac aggcacgttg cgcttgctga
     6421 gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta agcattgcca
     6481 gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc tcggtattta
     6541 aactgttctg gcgacccgaa ggttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aagtcgcagg gtgtacaact cactaaccca acgccttatt acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgtg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat agcaccgtgt catgttgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgcattaccg gaacaccgtt tgaagcactg gtgcggcaga aagtgcctgc aaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgacaaagac
     7141 ggggagtgca ttgatgcgtt aggcgcgatg ggatttggat cgatagagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaagccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtagagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccaaat accccaggat tacgcacact gcaatatggt
     7501 gaagcgctgg atgatctctt aactgcgatt aaaaataaac aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctctggt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttctt
//
LOCUS       DAZFBR010000175         7943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFBR010000175 DAZFBR010000000
VERSION     DAZFBR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.175"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(116..1603)
                     /gene="yeeO"
                     /locus_tag="ACHJZ2_004555"
     CDS             complement(116..1603)
                     /gene="yeeO"
                     /locus_tag="ACHJZ2_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO4570532.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     FAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLTTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
     gene            1764..1839
                     /locus_tag="ACHJZ2_004556"
     tRNA            1764..1839
                     /locus_tag="ACHJZ2_004556"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1797..1799,aa:Asn,seq:gtt)
     gene            complement(1877..2827)
                     /gene="cbl"
                     /locus_tag="ACHJZ2_004557"
     CDS             complement(1877..2827)
                     /gene="cbl"
                     /locus_tag="ACHJZ2_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO4570533.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2929..3846)
                     /gene="nac"
                     /locus_tag="ACHJZ2_004558"
     CDS             complement(2929..3846)
                     /gene="nac"
                     /locus_tag="ACHJZ2_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO4570534.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4172..4247
                     /locus_tag="ACHJZ2_004559"
     tRNA            4172..4247
                     /locus_tag="ACHJZ2_004559"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4205..4207,aa:Asn,seq:gtt)
     gene            complement(4303..5238)
                     /gene="ldtA"
                     /locus_tag="ACHJZ2_004560"
     CDS             complement(4303..5238)
                     /gene="ldtA"
                     /locus_tag="ACHJZ2_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4570535.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5300..6379)
                     /gene="cobT"
                     /locus_tag="ACHJZ2_004561"
     CDS             complement(5300..6379)
                     /gene="cobT"
                     /locus_tag="ACHJZ2_004561"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO4570536.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(6391..7134)
                     /gene="cobS"
                     /locus_tag="ACHJZ2_004562"
     CDS             complement(6391..7134)
                     /gene="cobS"
                     /locus_tag="ACHJZ2_004562"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO4570537.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7131..7676)
                     /gene="cobU"
                     /locus_tag="ACHJZ2_004563"
     CDS             complement(7131..7676)
                     /gene="cobU"
                     /locus_tag="ACHJZ2_004563"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO4570538.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7828..>7943)
                     /locus_tag="ACHJZ2_004564"
     CDS             complement(7828..>7943)
                     /locus_tag="ACHJZ2_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO4570539.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         2191 a         2161 c         1723 g         1868 t
ORIGIN      
        1 ctgaactaca gaggaatcgt gagaacgagg cgaatattag cgatgcccac ccacaatgtc
       61 aaagcctgtt ttttaaattt gaaatcgttt gctgaaataa tctgcatttt gtcgtttatt
      121 ccgacacagc tggctttttt tcacactttt gcggttcggg tcgagggtat ttccacagcc
      181 aacgtccagt aaccattcgc cagtaaaaca gcacggcccg cacagcccag tcggcaaaca
      241 ttcccatcca gacaccaacc acaccccagc caagcatgat gcccagcaca taaccgacga
      301 caacccgaca accccacatg ctcaacatag aaacccacat agcgtaacgg gcgtcacgag
      361 cccccttaaa tccagcgggt agcacccatg aagcggacca aataggcata aataaagcat
      421 ttagccaaat cagaatcaca acgacatgtt taacctgtgg atcctgggtg taaaacgatg
      481 ccataacccc ggcaaaggga gccgttagcc aggcgatggc cgttaatcca agagtggtca
      541 gccaaaacac atgccgcaac tgaatctctg cttgcgctat ctgccctacc cccaaccttc
      601 ggcctgtaat gatcgtagaa gcagaaccga gcgcacttcc gggtaagttg ataagagccg
      661 caattgaaaa cgcgataaaa tttccggcaa taacactggt ccccattccg gcaacgaaca
      721 tttgggttaa taaccgacca ctggtaaata acactgattc gacactcgct ggaataccaa
      781 tccccatgac ttcccagata atgctaaaat tcagcggttt aaaatagctc tttaacgaaa
      841 tccttagcgc aggattaaaa ccaatcgcca gcacccacaa aattgcaact gcgccaatat
      901 aacgagaaat ggttaaaccc aacccagccc cgacaaatcc cagtcccggc caggagaaaa
      961 ggccgtaaat caatatgccg ctaataataa tattaagaat attcaggcta ccgttaatca
     1021 gtagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaatt agagtgatgg
     1081 cagctgctgg ataactgagt accgtcagct ccagataagt caacgccagt gctttaactt
     1141 ctgtcgtggc atcacccgcg acgaaatcaa taatttgctc gccaaaatga tgaataagcg
     1201 ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg
     1261 ctcgtcgtcg atcccgctta ccgagactaa atgccacaac gacagtagta ccaagatcga
     1321 tagcagcaaa aaaagccata atgaccatat tgaagctgtc cgccaatccc acgccggcca
     1381 tcgcatcttt tcccagccag ctgaccagaa aagtgctcag aacccccatc aacaggacac
     1441 aggcattctc catgaagata ggaacagcaa acggggttat ctcgcgccag aacaacactt
     1501 tgtagctctt gcgtttagcg tgccagcgag tgccgtgaac aacctggcgt aaagcagagg
     1561 agatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat aatgagggag
     1621 aatcagcaaa gctgcaaaga ttttcgccat caaattgtct gcaaatgcaa caaactgttg
     1681 atagaaacgg caaacagttg ggaaaattaa aaatcgggtt tgacaaaaga tttttcgccg
     1741 ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta
     1801 atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt
     1861 tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct
     1921 gccgcttgat atcctcaact gaaagtcctg cattgcaaag ttccagaaag cgccagacat
     1981 agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca tcgaaaagat
     2041 gccgcgtatc caggcggatt aaattctttt cctcttgttc accgctggat tgttcggcaa
     2101 ctaatccgat cccaagccca agagcaacat aggttttaat gacatcagaa tcctgcgcac
     2161 ttaatataat atctgccagc aaacctttgc gggcaaatgc gtcatcaata cgtgagcgcc
     2221 ccgtaatccc ctgtcggtaa gtgattaacg gccacttcgc tattgattcc agcgtcaatg
     2281 gtgtaatttg cgtcaaggga tgatcgtgtg gaacaagcaa actatggtgc caacgaaacc
     2341 acgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag
     2401 cttcaccatt atgcaacaac gtcgcaattt cctgtggcgt cccctggatt agctcgagcc
     2461 gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct
     2521 gagtatgcgt cgttgcaata gtgagaacgc cagacgtatc gttggtaaac aggtctgcaa
     2581 gccgacgaac attactggct tcattgagaa tacgttctgc aatgaccagt aatgctttgc
     2641 ccggttcagt catgcccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaagtt
     2701 catcctccag ttcccgaata tgacggctga cgcctgactg tgaggtaaaa agcatattcg
     2761 caacctctgt caggttgtaa tcctgacgtg cagcttcgcg gattatcttt agttgttgga
     2821 aattcacggt aaactccggg cagttcagat atcccgttat tgttaaagtc taacgcccgg
     2881 cataacaaat aataaaaacc cgcatcttat tccatcccga tataacgctt agctcaccaa
     2941 ttgccactgc cttttttcca tcactggaga actaatcact gacattaaca actctttcac
     3001 tgcctgtgcc tgtggcgata agttcgctct ggcgggtaaa tttaatgaca aagagagact
     3061 catggaaggc gtggtaatgc gtgacatcca cccatttact gcgccacata acgaacgcgc
     3121 ggccgattcg ggtaatactg caacgcccat gccgctggca atcgctgcgg taagcgtagc
     3181 aatagactca atttcaccaa tgacttttgc cgtgagccgc cgtagggaaa acgcctcatc
     3241 aacacgaagt ctaacagcgc tgtaatcact ggggagaaaa aggttcattt gtgcaatagc
     3301 attaacatca acgctttgcc ccgggcaatc ttgagttcct accaggaaaa gatcttcttt
     3361 cagcaaagcc tgactggata caccagccac aggggaatgc tcataaatca ccgccatatc
     3421 gagttggtga tttatcagtt tttcgttaag cactgcaccg ctattttcat gaagatagat
     3481 aacgacctcc gggaattcag cgcgaaccgc ctgtaataag ggcatggtga tggatgacgc
     3541 agcggttcct ggcgcaaagc caatcgagac ttgccccgat aatgcctgac caacgttatg
     3601 caccgccagt tgggcctgct cacactgacg taaaatggcc cgcgcatggg tatagagaat
     3661 ttttccggcg tctgttggcg taacgccccg ttttgtgcga atcaaaagtt gttgatttaa
     3721 ctcaccttcg agtgtggcaa cctgctggct gagcgctggt tgtgcgatat gcaatacttc
     3781 agcagcctga gtcaggctac caatatctac aatttttacg aagtatttca ggcgtctgaa
     3841 gttcatgttg cctccggttt ttgagaatcg gcccaagtgc cgccattact tacaaccaga
     3901 ttgcaagatg cttgccagtt ttattttggc gttgatgtac aagctaacca actgtcaaat
     3961 aagagattat gatagattcg tcattggctc ttttcaccag cagtcgcgtt cccctgccct
     4021 ataaaaggag ggtatgcacc acgatggttc attaccaata agattgaaat ctcatcactt
     4081 tgttgaaatt gacagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca
     4141 ctgccattaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg
     4201 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc
     4261 ctgctttcta gcaggctttt tgctttctaa ttaccaacgc tcttaaaaca tctgtcttga
     4321 accagaacta atttgcacag gcattcccga tcgacgttgc aacgcagcat ttgcgcgatt
     4381 cacatcaact tcttgcccgt tgataaacgc ccgcaaagat ggggttaccg gcaatggcac
     4441 ttttcggtca gactcatatt cagcacgatt gcgcgacagc ggctcatgaa cttccagcca
     4501 ctttgaacca tccggttccg ttgtgtattt cactggctga tcaataattt gcacacgagt
     4561 acctacagga acattatcaa acagatattt gatatcgtca ttgcgcagac gaatacagcc
     4621 ctgacttacc cggagcccaa taccaaaatt ggcattggta ccgtggatgg catatagcct
     4681 gccaatataa atcgcgtaca gccccatggg attatcgggc cccgcaggaa caaatgcggg
     4741 caaactctcc cctcgtttcg catattcgcg ccgggtgttc ggcgttggcg tccaggttgg
     4801 tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc ggggtttctc gcccagcctg
     4861 gccgatacca ataggaaaga cttccacagt attactgtct ggtgggtagt aataaagacg
     4921 catctcagcg acgttaacca caatcccttt acgaacagtg acgggcaaaa tcagttgctg
     4981 cggaatggtg agttgcgaac ctgacttcgg taagaagaca tcagcgcccg gattcgcttc
     5041 cagcatgtta cttaaccctt gcccgtattg tgcggcaaaa gtctccagcg gctgggtatt
     5101 gtgatcagga acagttacag taagcgactg ccccactaaa cggctaccct ctggaggtaa
     5161 tggataagtt accgccaggc tagtatggct ggcaaaaagc agagcaaatg agcaaagaat
     5221 atttacacga cgcatcatgt ccctttccta agtcgcgaaa gctatcggtt aagtatagct
     5281 tttatcagac ttttcgtttt taactgttca aatcagaagt cgtattcccc ggtagaacaa
     5341 ttttactggc agcaagttcg cccatgttgt tgtatatcgc acaagcagct tcgatgatgg
     5401 gcatcgccag agctgcgcca ctcccctcac ctaaacgcat atccatattg agaaaaggct
     5461 ccagccccaa atgcgacagc gctatacgcg cccctttttc tgccgacagg tgagaaggaa
     5521 tgagatacgg tttgattgca ggagacatct ggcaggctgc gagcgcagca gcataagaaa
     5581 gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc gcctaacatc acgccagcca
     5641 ttccgaccaa atcaaatcca cccacttttg ccagaacatt aacgccatcc tgcggatttg
     5701 gctgattcaa cgtaatcgcc cggcgcacaa catcaatttt attagccagt ttatctgtcg
     5761 gcaggtttgc gccaatccca accacttctt caggatcccg gccagtgatt gtgctgacta
     5821 ttgctgctgc cggtgtcgtg tttgccatcc ccagttcacc tacaccaaac agcgtgacac
     5881 cgttttttgc cagctcccgc gtataacata tgacgtccaa aagtaacttt tcagcctgac
     5941 ggcgactcat tgccggagct gaagcaatat tgccgctacc tcgtgcgaca cgcatgttga
     6001 taagcccggg gataggctca gcagtatcaa taccaacatc aattacgtgg acgttagcgc
     6061 ccgcttgtgc tgccagcaca cacacgccgg ttgttccgcg ggtcatattt tcagcctgaa
     6121 tggctgtcac ttcttttggg gaaatagcga ccccttcctc ccagacgccg tgatcggcac
     6181 acataaccag taccgctttt ttgcccacat gcggtatgcc attcaacccc ggcattcccg
     6241 ccagttgtac gccaagcgcc tccagccttc ccaggctacc aacaggtttg agtaacccgt
     6301 caatatgccg ttgtgcacgc gacatagcgg caggatcgat ggcagggatc gtattcagta
     6361 aattggcaag tgtttgcatg tcacgtctct ttatagcagt gccagcagga agaccagttc
     6421 accaagctct atggctgctc ccagcgtatc gccggtttgc ccgcccagcg tacgtttgag
     6481 aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc atagcagcca caccatgcat
     6541 acccggcaat aatacggcag caaaaatagc ggctaagccg agtgtgacgc aggtttgtcg
     6601 cccatcaatt ttgccaatga acacattgcc aagcccttct tcccgggcat aacgatgacg
     6661 atacatcaat aatgcggcag tcccgcggct gaccgcacat gctgccgcca gcgatgcaag
     6721 gatcggttcg ccacgcagag ccaactcgct taacaccaaa atctttgcca gtacaacaaa
     6781 aatcaatgcc agaccgccgt gggtacctaa acggctatca cgcatgattt ccaacatgcg
     6841 atcgcggcta cgtgcagaaa atacgccatc acaggtatca gcgaggccgt ccagatggaa
     6901 tcccccggtc atcagcacca gcacaagtac gctaaacagt gccgccagtg gtgcgccaca
     6961 ccatgcctgc agcaccatga agaccagccc gctaatcgcg ccaagcaata atccaatcaa
     7021 aggaaaagta ataataccgc gagaataatg ctcgaaatcc agtccctggg accagcgacg
     7081 cggtacaggc agacgcgtaa taaatgagag catcgcccaa aataatttac tcatttgatt
     7141 tttactccaa tacccgaaac caccagccat acttcatttg ccgcagcggc caactgctga
     7201 tttacccgcc cggcaatatc acgaaaatgt cgtgccagac gactctccgg cacaatcccc
     7261 attcccactt cgttagtcac taatacaacc tttgcggggc aacgttggca ggcagcaatc
     7321 aacgactgaa tctcagcatt aatcgcctgt tccatcgcct gataatccca ttcatcaggg
     7381 tctttatcgc cgccataatc aaacaacaga ttagtcacca ttgtggtaac gcattcaagc
     7441 aacacaacct cattcgggtt aatgtctgca tgaattaatt catcaagatg ttgccagcgc
     7501 tccactgtgc gccagtgctc cgggcggcct tgccgatgat gttctatccg tgcagccatc
     7561 tcatcatcaa ggatttgcga ggtagcgata tacagaacct gtgaagagtc cccaataagc
     7621 gcctctgcgt ggcgactctt cccgctccgt gcgccccccg tcaccaaaat catcataccg
     7681 actcctgatt caaatgtatg atggtattca ttgaacagaa caatggatcc cccaccgttg
     7741 ctgaccgcaa aacatttacg cgattaatac tgcgcgtaat ataattaaaa atccagcatt
     7801 ctcaatccat tacgaaataa gatggcatta cggaatagcc agactttctc gtgttaacgt
     7861 ccgacaaaat gcgtcattga cagcggtttc tgtggattga cacatttcat tcagataatg
     7921 aattaatgct actgccggaa caa
//
LOCUS       DAZFBR010000176         7759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFBR010000176 DAZFBR010000000
VERSION     DAZFBR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.176"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(195..458)
                     /gene="cedA"
                     /locus_tag="ACHJZ2_004565"
     CDS             complement(195..458)
                     /gene="cedA"
                     /locus_tag="ACHJZ2_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO4570540.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            641..2902
                     /gene="katE"
                     /locus_tag="ACHJZ2_004566"
     CDS             641..2902
                     /gene="katE"
                     /locus_tag="ACHJZ2_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO4570541.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2949..3707)
                     /gene="chbG"
                     /locus_tag="ACHJZ2_004567"
     CDS             complement(2949..3707)
                     /gene="chbG"
                     /locus_tag="ACHJZ2_004567"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO4570542.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMNFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3720..5072)
                     /gene="celF"
                     /locus_tag="ACHJZ2_004568"
                     /gene_synonym="chbF"
     CDS             complement(3720..5072)
                     /gene="celF"
                     /locus_tag="ACHJZ2_004568"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO4570543.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5178..6020)
                     /gene="chbR"
                     /locus_tag="ACHJZ2_004569"
     CDS             complement(5178..6020)
                     /gene="chbR"
                     /locus_tag="ACHJZ2_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO4570544.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6028..6378)
                     /gene="chbA"
                     /locus_tag="ACHJZ2_004570"
     CDS             complement(6028..6378)
                     /gene="chbA"
                     /locus_tag="ACHJZ2_004570"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO4570545.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6429..>7759)
                     /gene="chbC"
                     /locus_tag="ACHJZ2_004571"
     CDS             complement(6429..>7759)
                     /gene="chbC"
                     /locus_tag="ACHJZ2_004571"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO4570546.1"
                     /translation="VLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLINNVFLS
                     FGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKV
                     DALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVRRN
                     WVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLAS
                     LGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALAA
                     GKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEP
                     ILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNT
                     NGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
BASE COUNT         2098 a         2021 c         1727 g         1913 t
ORIGIN      
        1 aaaaatagct catcatgatt agcaaaactt aaccatttta aaataaataa acaattaaag
       61 aaaaaagatc acttatttat agcaatagat cgtcaaaggc agctttttgt tacaggtggt
      121 ttgaatgaat gtagcaatga aatagagaat ttcaggccat gtaactcccg gcaaaaccgg
      181 gaggtacgta atctttactc agtcacttcc ccttcctggc ggatctgatt tgcccaacgc
      241 tgggcggatt caggcacagt aaacgccggt gagcgcagaa atgactctcc catcagtaca
      301 aacgcaacat atttgccacg cagcatccag acatcacgaa acgaatccat ctttatcgca
      361 tgttctggcg gcgcgggttc cgtgcgtggg acatagctaa taatctggcg gttttgctgg
      421 cggagcggtt tcttcattac tggcttcact aaacgcatat taaaaatcag aaaaactgta
      481 gtttagccga tttagcccct gtacgtcccg ctttgcgtgt atttcataac accgtttcca
      541 gaatagtctc cgaagcgggg tctggctggt ggtctatagt tagagagttt tttgaccaaa
      601 acagcggccc tttcagtaat aaattaagga gacgagttca atgtcgcaac aaaacgaaaa
      661 gaacccacat cagcaccagt caccactaca cgattccagc gaagcgaaac cggggatgga
      721 ctcactggca cctgaggacg gctctcatcg tccagcggct gaaccaacac cgccaggtgc
      781 acaacctacc gcctcaggga gcctgaaagc ccctgatacg cgtaacgaaa aacttaattc
      841 tctggaagac gtacgcaaag gcagtgaaaa ttatgcgctg accactaatc agggcgtgcg
      901 catcgccgac gatcaaaact cactgcgtgc cggtagccgt ggtccaacgc tgctggaaga
      961 ttttattctg cgcgagaaaa tcacccactt tgaccatgag cgcatcccgg aacgtattgt
     1021 tcatgcacgt ggatcagccg ctcacggtta tttccagcca tataaaagct taagcgatat
     1081 caccaaagcg gatttcctct cagatccgaa caaaatcacc ccagtatttg ttaggttctc
     1141 taccgttcag ggtggtgctg gctctgccga taccgtgcgt gatatccgtg gctttgccac
     1201 caagttctat actgaagagg gtatttttga cctcgttggc aataacacgc caatcttctt
     1261 tatccaggat gcgcataaat tccccgattt tgttcatgcg gtaaaaccag aaccacactg
     1321 ggcaattcca caagggcaaa gcgcccacga tactttctgg gattatgttt ctctgcaacc
     1381 tgaaactctg cacaacgtga tgtgggcgat gtcggatcgc ggcatccccc gcagttaccg
     1441 caccatggaa ggctttggta ttcatacctt ccgcctgatt aatgccgaag ggaaggcaac
     1501 gtttgtacgt ttccactgga aaccactggc aggtaaagcc tcactcgttt gggatgaagc
     1561 acaaaaacta accggacgtg acccggactt ccaccgccgc gagttgtggg aagccattga
     1621 agcaggcgat tttccggaat acgaactggg cttccagttg attcctgaag aagatgaatt
     1681 caagttcgac ttcgatcttc tcgatccaac taaacttatc ccggaagaac tggtgcccgt
     1741 tcagcgtgtc ggcaaaatgg tgctcaatcg caatccggat aacttctttg ccgaaaacga
     1801 acaagcagca ttccatccag gtcatattgt tcctggtctg gacttcacca acgatcccct
     1861 gttgcaggga cgtttgttct cctataccga tacacaaatc agtcgtcttg gtgggccgaa
     1921 tttccatgag attccgatta accgcccaac ctgcccttac cataatttcc agcgtgacgg
     1981 catgcatcgt atggggatcg acactaaccc ggcgaattac gaaccgaact cgatcaacga
     2041 taactggccg cgcgaaacac cgccgggacc gaaacgcggc ggttttgaat catatcagga
     2101 acgcgtggaa ggaaataaag ttcgcgagcg cagtccatct tttggcgaat attattccca
     2161 tccgcgtctg ttctggctaa gtcagacgcc attcgagcag cgccatattg tcgatggttt
     2221 cagttttgag ttaagcaaag tcgttcgtcc gtatattcgt gagcgcgttg ttgaccagct
     2281 ggcgcatatt gatctcactc tggcccaggc ggtggcgaaa aatctcggta tcgaactgac
     2341 tgacgaccag ctgaatatca ccccacctcc ggacgtcaac ggtctgaaaa aggatccatc
     2401 cttaagtttg tacgccattc ctgacggtga tgtgaaaggt cgcgtggtag cgattttact
     2461 taatgatgaa gtgagatcgg cagaccttct ggccattctc aaggcgctga aggccaaagg
     2521 cgttcatgcc aaactgctct actcccgaat gggtgaagtg actgcggatg acggtacggt
     2581 gttgcctata gccgctacct ttgccggtgc gccttcgctg acggtcgatg cggtcattgt
     2641 cccttgcggc aatatcgcgg atatcgctga caacggcgat gccaactact acctgatgga
     2701 agcctacaaa caccttaaac cgattgcgct ggcgggagac gcgcgcaagt ttaaagcaac
     2761 aatcaaggtc gctgaccagg gtgaagaagg gattgtggaa gctgacagcg ccgacggtag
     2821 ttttatggat gaactgttaa cgctgatggc agcacaccgc gtgtggtcac gcattcctaa
     2881 gattgacaaa attccggcgt aaatatggat aaggtcacgt cccctgggga gtgaccttac
     2941 caccttactt acacatcaag ataactaccc aaccgataac cacgctcagc aattgcatat
     3001 ttcaacgacg ctgatgtcag cacttccagc tccgttaacc gtgggaaaca gtaagcactc
     3061 tggcgaatcg tattatcgac aaacgccgga tggcacatga cttccagcga gcgttccccg
     3121 cgatggctgg aatcatcaag cacttgcagg aacagtgcct cgctaatctc ttcaccataa
     3181 aatgcgctac tgaatccgtg cgaactgcgc agattagccg gtaaatcacc agcgttagat
     3241 agcggctgac ggtcgatgcg caacgcaatc ccctcttcag ccgcaaacct ggcaacaata
     3301 gggaaaatct gcgggaacat atgcacatga tgatggctat caagatgcgt aggtttgcgt
     3361 ccaaatagct caatgaaacg caaatactga ctggcaagct cctgagtaat ttcttccagc
     3421 ggtaaagcat cttcttctgc caactgccag atccattttc ccagcacacc atcgcgggtt
     3481 aaccccggca tagctgtcag tggcttgccc atagtaagga caaagttcat ccctatggcc
     3541 agactcggtt catcacgact caactgcacc gcatggtcaa tagcctgccc attcaccagc
     3601 gccgtcgtcg atgtgacaat cccattgcga caggcctcga taatgccgta gttctgacct
     3661 ttgcttaagc caaaatcatc ggcattaaca atcagtaagc gttccataat cagcctcggt
     3721 taatgtgctt ttttaagctc tgcgatgcag tcggcaaaat ttggcagcca tttctcgtgc
     3781 gccagaatca tctcgcgtgc cagcagctca gcatcgcgat cggaatgcac caacggacta
     3841 aggtttagcg ccagtaacac atcgttaaat tctccgctaa gtgcggcgtt gctggcagca
     3901 atctcgaagc ctttaatggt gtgaatcagc cccatcactt tatcatcgaa atgcgtaatg
     3961 cgcggatgtg gcgtcgcgcc atcgcgcccc agcgtacagg tcatttctac cgcccagtct
     4021 gccggaatat tatcaatatg cccatgatgc gggatattaa cgtaatgttc tgcttgctta
     4081 tcgttgtaga tagcgttgat cacttcgcac gctgcatcag agtaataagc cccaccgcgc
     4141 tgttccagtt ctttcggctt aactttcaac tccgggtttt tatacagctc aaaaagttgt
     4201 ttctcgactt tctgtactac ctgcgctcgt gcgccgcctt tgtagtattc gcccatttca
     4261 atagccagca tctctttttg cttgaagtaa taaagcaaat aagaacacgg caacagattc
     4321 agagaacgaa ttaagccttc gctaaatggc agatcgaaaa tatttttaac gccagatgct
     4381 tttaactgcc cggatgccac accatcaagc aattcggcaa agcgcgactt gccatttacc
     4441 agcacatcct taatgaacac catatggttg aggccgaaca gatcgataga taaatcatcg
     4501 ctgtctttca gcatcagaac atcgcgaata aacatcttca tgccgatcgg aatattacac
     4561 acgccgataa agcgtttaaa tccggtatga cgataaacgg cttcagtgac cattcccgcc
     4621 gggttagtga agttaatcac ccatgcattc ggacaaagtt cttcgacatc ttttacgatg
     4681 tcaaaaatca ccggaatggt acgcagacct ttaaacagac cgcccgcgcc gttggtttcc
     4741 tgaccaagat aaccatgact taatggaata cgttcatcca gttcacgcgc cggtaattgg
     4801 ccaacgcgca gttgggtagt aacgaaatca gcatctttca atgcttcgcg gcgatccagc
     4861 gttttataaa gcttcatcgg gacgccagcg ttatcaatca tccgttggca gagatcgaaa
     4921 ataatatcca gtttagcttt gccaccttcg acatccacca gccataattc gctgaccggc
     4981 aattcgtgat aacgcttaat aaatccttcc agtaactccg gggtatagct gctcccgcca
     5041 ccaatagtga cgacttttaa tttctggctc ataatttctc ccttcagtac agaatactga
     5101 tatctggcat atctgccccc cccggacata aataatccag caacaggaca gatatgtgaa
     5161 ttgtcaggta taacgactta ctgattaaat tcagtcaatt tcttacgata gctcttaggc
     5221 gtaaaggacg ttaatttctt aaacgtttta ataaacaaac tcgggctact ataaccggcc
     5281 tcaaacgcaa tatccgtcac tgaatagttg gtcatttcca gttgtttttt ggcaaaatta
     5341 atacggattt cattaataat ctgcattggc gttttgccat aatatcgttg agtcgcccgc
     5401 gtcaaatatt cctgtgattt ggctgacaac gtcaccatat tctccagcgc cgattcacta
     5461 aactgctctt tatcatgcat cttttctacc gtacttttca gccactgcgg tacatcatca
     5521 atcacctgtt cttcgcggta atgacgtaaa cggttaatga cataaaaagt aaccatctca
     5581 acaaactctt ccagccctgt ttcgcggaaa ttcaatgaag aaatcactgt ttccacatag
     5641 gtgagaaaag cgttattggt ccggtatacc tgcgaagcta caaagcaata aggcaacaat
     5701 ggcaggtaat gctgctcaaa aaagcgttta ctgatcccaa cgttcaatat gcgcgtggca
     5761 ccaaactcat aaaaactttg atggtgcgaa cctaacggaa taaaaacaaa atcgccccgt
     5821 tccagtaaca cgcgcttacc gttaatctcc tggaaataac gcccggttaa taccagagta
     5881 aattcataat agtcgtgctg atgcagtccg ctgatactct cagttttgtt atagataaac
     5941 acatggaagt ttttgccatt aaacaactgc tgttctcggg cagtggcaat ttccggcgcg
     6001 ttaatcactg gctgcatcat cgactcctta tgccttcagt ttttcatgaa gctcaattaa
     6061 ttcagtaatc agttcacgcg caagcatgga cgtcattaaa tgatcctgag cgtggaccag
     6121 caccagactc actttcatct taccttcgcc cgcatcgcct tcaatcagtt tcgtctgtac
     6181 cagatgcgct tcattcaatg ccatccgtga ctgatccatc atggcttttg ctgcggcaaa
     6241 atcgccctgc ttcgcctgtt tcagtgcggc atacgccagg ctgcgcgctt gtccggagtt
     6301 gatgataagc cccatcacca cttcttccag ctcttcagct tccgtttgcg tatcgggaat
     6361 attatcgaga tccatcatac atcgttcctc tcttttctta ccggcacgat tacccgtacc
     6421 ggcatcgatt aaaatttcag ggcgttagcg atatcttctt cgctctcttc tttatcaatc
     6481 gcattttgtg ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatacca
     6541 aggttgaaga gtgcaaccag caatgcgacg acgctaccgt tggtgttaaa gaaggctccc
     6601 agaccggttg gcatggtcca cggtgcaata ttggtcaccg gaggaataat gcccatgtag
     6661 tacgctgcca gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc
     6721 accgggttca tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg
     6781 gacggcagcg ccagttttgc cacctgacga taatcagcac ggcgagaggc aataaagata
     6841 gccaggatca ggcctaaagt cgcaccactg cctccaagga aaataaagga gtccagcatc
     6901 ggcttggccc agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat
     6961 tgctgatagg tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg
     7021 gtcagcgcca gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag
     7081 gcccagccca ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc
     7141 tgatggaagt tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga
     7201 ataataaagc cgggaattaa tgccgagaag gaacgcgata ctgaagcagg tacgctgtcg
     7261 ggcagtttaa tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg
     7321 ccaataataa tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag
     7381 gcctcaccga cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc
     7441 gccagcgcat cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt
     7501 aaagacatta tccctaatgt tccgttatat acgttgccgc caataccttt cagaccatta
     7561 agtgtttcaa tggttgaggc gtcgaggcga atacctaagg aataaaaaaa cgacccctcc
     7621 ccaaagctta gaaaaacgtt gttaattaat acaaacatgg cccccgcaag ggttaacggc
     7681 attaagcgaa taaagccatt tttgattgca ttaacgtgtg gctgctttcc tattttaact
     7741 gcaaaaggga ggagtacct
//
LOCUS       DAZFBR010000177         7731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFBR010000177 DAZFBR010000000
VERSION     DAZFBR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.177"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..156)
                     /locus_tag="ACHJZ2_004572"
     CDS             complement(<1..156)
                     /locus_tag="ACHJZ2_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HFO4570547.1"
                     /translation="MVVFFLKIPNLDFAVYLRPFYVPYMPHRYPAAKINKMPKGSVPA
                     LQQEMLRR"
     gene            216..1498
                     /gene="ydfJ"
                     /locus_tag="ACHJZ2_004573"
                     /pseudo
     CDS             216..1498
                     /gene="ydfJ"
                     /locus_tag="ACHJZ2_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1587..3047
                     /gene="ydfI"
                     /locus_tag="ACHJZ2_004574"
     CDS             1587..3047
                     /gene="ydfI"
                     /locus_tag="ACHJZ2_004574"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO4570548.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLHQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3083..3286)
                     /gene="ydfZ"
                     /locus_tag="ACHJZ2_004575"
     CDS             complement(3083..3286)
                     /gene="ydfZ"
                     /locus_tag="ACHJZ2_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO4570549.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3463..4149)
                     /gene="rspR"
                     /locus_tag="ACHJZ2_004576"
                     /gene_synonym="ydfH"
     CDS             complement(3463..4149)
                     /gene="rspR"
                     /locus_tag="ACHJZ2_004576"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO4570550.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4238..4984)
                     /gene="ydfG"
                     /locus_tag="ACHJZ2_004577"
     CDS             complement(4238..4984)
                     /gene="ydfG"
                     /locus_tag="ACHJZ2_004577"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO4570551.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5121..7166
                     /gene="dcp"
                     /locus_tag="ACHJZ2_004578"
     CDS             5121..7166
                     /gene="dcp"
                     /locus_tag="ACHJZ2_004578"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO4570552.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7211..7729)
                     /gene="ydeJ"
                     /locus_tag="ACHJZ2_004579"
     CDS             complement(7211..7729)
                     /gene="ydeJ"
                     /locus_tag="ACHJZ2_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO4570553.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1935 a         1812 c         1964 g         2020 t
ORIGIN      
        1 acgtcgcagc atttcttgtt gcagggcagg aacagaacct tttggcattt tgttgatttt
       61 ggctgcgggg tatctgtggg gcatatatgg gacataaaaa ggcctcaaat aaacagcaaa
      121 atcgagatta gggattttta gaaaaaatac aaccatctga aaaaacttag aaaacaccca
      181 aaaacccaca gaatagtaaa aaacatctat gaattatgga tttccagtta tattcgctcg
      241 gcgcagcgtt agtgtttcat gaaatatttt ttcctgaatc atcaacggca atggcgttaa
      301 ttctggcaat gggaacctac ggtgcaggtt atgtggcgcg tattgtcgga gcatttattt
      361 tcggcaaaat gggcgacaga atagggcgta aaaaagtgct ctttattacc atcaccatga
      421 tggggatctg taccacctta attggagtgt taccgaccta tgcacagatt ggtgtttttg
      481 cccccatctt gctggtgacg ctgcgtatta ttcagggatt gggtgcaggt gcggaaattt
      541 ccggtgccgg tacgatgctg gcggaatatg cgccaaaagg taagcgcgga attatctcct
      601 catttgtggc tatgggaact aactgcggaa ccttgagcgc aacggcaatc tgggccttta
      661 tgttcttcat tctcagtaaa gaggaactgc tggcgtgggg atggcgtata ccgttcctgg
      721 cgagcgttgt cgtgatggtc tttgctatct ggttgcgtat gaatctgaaa gaaagcccgg
      781 tctttgagaa ggttaacgac agcaaccaac cgacagcaaa acctgcacct gctggtagca
      841 tattccagag caaatccttc tggctggcaa cagggctgcg ttttggtcag gcaggtaact
      901 ccgggttaat tcagactttc cttgcaggct atttagtaca gacgttattg tttaacaaag
      961 caattccaac agatgcattg atgatcagtt cgattcccgg ctttatgacc attccgttcc
     1021 ttggttggtt atccgataaa attggtcgcc ggatcccgta tattattatg aatacctccg
     1081 cgattgtgct ggcatggcca atgctttcta tcatcgtaga taaagctatg ccccgagcac
     1141 cattatggtt gcactgattg tgattcataa ctgtgcggtg ctgggattat ttgctctgga
     1201 aaacattacc atggcagaaa tgttcggctg taaaaaccgc tttacccgga tggctatttc
     1261 taaagaaatt ggtggtctta tcgcttccgg ttttggtcct atcctggcgg gtattttctg
     1321 caccatgacg gaatcctggt atccgatcgc cattatgatc atggcatatt cagtgattgg
     1381 tttaatctct gcgctgaaaa tgccagaagt gaaagaccgt gatttaagtg cgctggaaga
     1441 cgctgcggaa gatcaaccgc gtgttgtaag agctgcgcaa ccttccagaa gtctgtaaat
     1501 cctcaatccc ttctcttacc ggagagggga tttttattca tataacaaaa catgtagctt
     1561 gccatattta aatttaagga attgttatgg gaaataattt gttatcagca aaagcgacac
     1621 tccctgttta tgatcgtaat aacctggccc caagaattgt tcatttaggc tttggtgcat
     1681 ttcaccgtgc gcatcagggc gtgtatgccg atattcttgc tacagaacat ttcagtgact
     1741 ggggatatta tgaggtcaac ttaatcggcg gcgaacagca aattgccgat ttacatcagc
     1801 aagataatct ttataccgtt gcggaaatgt cggccgatgc gtggacggct cgcgtcgttg
     1861 gcgtcgttaa aaaagccttg cacgtacaga tagatggctt agaaaccgtg ttggctgcga
     1921 tgtgtgaacc gcaaatcgcg attgtctctc tgacaatcac cgaaaaaggg tatttccact
     1981 ctccggcgac cggacagtta atgctcgatc acccgatggt cgctgccgac gtgcaaaatc
     2041 cccaccagcc gaaaacagca acaggggtga ttgttgaggc gctggctcgc cgtaaagcgg
     2101 caggacttcc cgcatttacc gtcatgtcat gtgacaacat gccagaaaac ggtcatgtta
     2161 tgcgtgacgt tgtcacttcc tacgcacaag ccgttgatgt aaaactagca caatggatcg
     2221 aagataacgt gactttccca tcaacaatgg tggaccgtat tgtgcccgca gtgacagagg
     2281 atacgctggc gaaaatcgaa cagcttaccg gtgtgcgcga tcctgctggc gttgcctgtg
     2341 aacctttccg ccagtgggta atcgaagata actttgttgc cggacgtccg gaatgggaaa
     2401 aagcgggagc cgaactggtt agtgatgtgc tgccttatga agagatgaag ttgcgcatgc
     2461 tcaacggcag tcattcattc ctggcgtatc tgggttatct tgccggatat cagcacatta
     2521 atgactgtat ggaagatgaa cattatcgtc atgcggcgta tgccttgatg ttgcaggaac
     2581 aagcgccgac gctgaaagtg cagggcgttg atttgcaaga ttacgctaac cgattaattg
     2641 cacgctatag caacccggcg ttacgtcatc gaacctggca gattgcgatg gatggcagcc
     2701 agaaattgcc acagcggatg ttggattctg ttcgctggca tctggcgcat gacagcaagt
     2761 tcgatctgct ggcgctgggc gtcgcgggtt ggatgcgtta tgtcggtggt gttgatgaac
     2821 agggaaatcc gatagaaatc agtgacccac tgttacctgt tattcagaag gctgtacaaa
     2881 gtagtgccga agggaaagcg cgcgtccagt cattgctggc gattaaggcg atttttggtg
     2941 gtgatttgcc agacaatagc ttgtttactg caaaagtgac ggaagcgtac ttgtctttat
     3001 tagcgcatgg tgcgaaagcg accgtggcga aatattccgt gaagtaaaaa aagcaatgcc
     3061 gccatcggcg gcattcacac gcttagttca tgccgagacg aatcaggtcc agtggtgcca
     3121 gtttctcttc acaaccttca acaactacgg tttttccgcg gcggatttgc tcagcggtca
     3181 gaccttcagt atcaatcgac aggatcttgc cagtgtgacc ggtgccgcta accataacac
     3241 ggctgccagt ggtgattgcg ttacggttac gatcgtaggt cgtcataggt gttttctcct
     3301 ttctgattta cagtcgaagc ccacgtaacg ggcgcaatat aaatactcct gtcgagtgaa
     3361 atattttttg tttttgatca agctcacatc ttttttgatg catgcatgag ccataaggcg
     3421 ggggaaaaga ctctcacccc ggatgggatg agaggaaatg aattactctt cgctaaacca
     3481 gtcgctattt tcctggcgga tctggcgcac ggattcgctg atttcctgca aatgttgcgt
     3541 cattgcacgt tctaccgcat cgccatcacg tttttgcagg gcagagaata tatcaagatg
     3601 ctggcgtaac agcatttctg gtggagaaac atggtcgaaa ctcatatagc gcacgcgatc
     3661 aacggtcgct ttcaggttct caatggtatc ccacgccagt tgacagtcgg caatctgcgt
     3721 caggagttga tggaagttgt catcaagttc aaaaaaatca tccagttgct tgcgctcaat
     3781 ggcaatgcgt tgctggtgaa gattttgttc cagttggtag cactggcttt cggtaatcat
     3841 actcgccgcc cgacgcgcca ccgcgcactc aatggcctga cggataaaac tgccgttgcg
     3901 cacctgggcc atggaaattt tgttgacgta gctgccacgc tgcggacgaa tttgaatcag
     3961 gccgttttcc gccagtttaa taaaggcttc acgaaccggc tggcgtgaca cattgaaacg
     4021 aacagaaact tctttttccg acaacggtgt gcctggtgca atcaggcaat ggacaatgtc
     4081 gcgacgaaga atacgataaa tctgctgatt gacaggctgt gtgggattaa gttgcgtttc
     4141 gacggtcatt cgttcttgct tgtgagtgag ttaactgcat gagtctacca cttttttgtg
     4201 acaagcaata acccggcagt tacaccgggt ataaaaatta ctgacggtgg acattcagcc
     4261 cggcatagct ttgggtaacc ggcatcattt ccagggtatt gatattgacg tgagcaggca
     4321 gcgttgacac ccaccagacg gcttcgctga catcttctgg cgttaatgca acggtatttt
     4381 gataggtttt ttccgcttta ccgtcatcgc ctttaaagcg gacattggaa aactcggtgc
     4441 cacccaccag acccggttcg atgtcggtga cgcgcaccgc cgtaccatgc aggtccgtac
     4501 gcagattcag gctaaactga cgaacaaacg ctttcgtcgc accgtaaacg ttaccaccgg
     4561 catacggcca gctacctgcc gttgagccaa tgttaataat atgaccatga ttacgttcaa
     4621 ccatacccgg taagacggcg cgcgtcatat ataccaggcc tttgttgttg gtatcaatca
     4681 tcgtttccca gtcttcaatg ctggctttat gcgcaggctc catgcccaac gccagaccgg
     4741 cattatttac caggatatca atattgcacc actcggcagg aagcgatgcc agcatctctt
     4801 caatagcggc gcggttgcga acgtccagtt gggcgatgta cagattatct cccagttcgt
     4861 cttttaactc ctgcaaccgt tcctggcgac ggccagtggc gataacttta tgcccttgtt
     4921 gaataaaacg acgagtaatg cattcaccaa aacctgccgt tgctccagtt actaaaacga
     4981 tcatctcact gttcctcaac gcttttgtgt gtcaataaca tagcacgtga tgcttaagag
     5041 cgggtaatac tggtttgcaa gattgcactc cgttatcagt aagcctacgc tatggaacac
     5101 ctcgcgttca ggagaagaaa atgacaacaa ttaatccttt ccttgtgcaa agcacactgc
     5161 cgtatctggc tcctcatttt gatcaaattg ccaatcatca ctatcgcccg gcattcgatg
     5221 agggaataca gcaaaagcgg gcagaaattg ctgccatcgc gcttaacccg caaacacctg
     5281 atttcaacaa tactattctg gcactggaac aaagcggaga attacttacc cgcgttacca
     5341 gcgtcttttt tgcgatgact gcggcgcata ccaatgatga attacagcgt cttgacgagc
     5401 agttttccgc tgaactggcg gaactggcta atgatatcta tctgaacggt gaattattcg
     5461 cgcgggtaga tgctgtctgg caacgccgtg aatctctggg gcttgatagt gaatccatcc
     5521 gcctggtgga ggtgattcat caacgttttg tcctagccgg agccaaactt gcacaagctg
     5581 ataaagcaaa attaaaagta ctgaatacag aagctgcgac actgaccagt cagtttaacc
     5641 agcggttact ggcagcaaat aaatccggcg gtctggttgt gaacgatatc gcgcagctgg
     5701 caggaatgag tgagcaagag attgcgctgg cggcagaggc ggctcgcgag aaaggtctgg
     5761 ataacaaatg gctgattccg ctgctgaata ccacccaaca accggcgctt gccgaaatgc
     5821 gcgatcgtgc gacgcgtgaa aaactgttta ttgcgggctg gacgcgagcg gaaaaaaatg
     5881 atgccaatga tacccgcgct atcattcaac gtctggtgga gatccgtgca caacaggcta
     5941 cattactcgg ttttcctcat tatgccgcat ggaaaatcgc cgatcagatg gcaaaaacac
     6001 ctgaagcagc actcaacttt atgcgggaaa ttgttccagc ggcgcgtcaa cgtgcgagcg
     6061 atgaattagc ctccatacag gcggttattg ataagcagca gggcgggttt agcgcgcagc
     6121 cgtgggactg ggcattttat gccgaacagg tccggcgaga gaaatttgat cttgatgagg
     6181 cgcagctcaa gccatatttt gaattaaaca cggtgttgaa tgaaggtgta ttctggaccg
     6241 cgaatcagct cttcggtatt aagtttgtcg aacgttttga tattcctgtc taccatcctg
     6301 acgttcgggt gtgggaaatt tttgatcata atggcgtggg gctggcgtta ttttacggtg
     6361 atttcttcgc ccgtgattca aaaagcggcg gtgcatggat gggcaatttt gttgagcaat
     6421 caacgcttaa tgaaacgcat ccggtaattt ataacgtctg caattatcag aaacccgctg
     6481 ccggtgagcc tgcgttgtta ctctgggatg atgtcataac cttattccat gaatttggtc
     6541 atacgctgca cggccttttt gcccgccagc gttatgccac gctctccggc accaacacgc
     6601 cgcgtgattt tgtcgaattt ccgtcgcaaa tcaacgaaca ctgggcaacg catccgcagg
     6661 tattcgctcg ctacgcccgg cattatcaga gcggggcagc aatgcctgac gaactgcaac
     6721 agaaaatgcg taatgccagc ctgttcaaca aagggtatga gatgagcgaa ctgcttagcg
     6781 ccgcacttct cgatatgcgc tggcattgcc tggaagaaaa cgaagcaatg caggatgtcg
     6841 atgattttga attgcgggcg ctggtggcag aaaatatgga tcttcctgct ataccgccac
     6901 gctatcgcag cagttatttt gcccatattt ttggtggcgg atatgccgca ggttattacg
     6961 cttatctgtg gacgcaaatg ttggccgatg atggttacca gtggtttgtt gagcagggcg
     7021 gattaacgcg tgaaaatggg ctgcgttttc gcgaggcgat cctttccaga ggtaacagcg
     7081 aagatctgga acgcctgtat cgacaatggc gcggtaaggc tcctcagatt atgccgatgc
     7141 tgcaacatcg tggcttgaac atataaacac caccaatggc tgtcctggag atatcgccag
     7201 gccagcctgg ttatagcagt aactgcagca gctgggcgag ggcaaacctc accgctaaag
     7261 ctaataccgt ttcgcagtcg ccagcaaaat gcataaccgc agtgtagttc tggcctttaa
     7321 tatgccacgc gaaccagacg gtacccgctg gcgtaccatc ttcaccgccc tccggtccgc
     7381 cgtagccggt aatggcaata ctgacatcag catccgcacg ctctatggca ccggttgcca
     7441 tttctgctgc cactttctca ctcaccgcag aatatcgttc aagagattgc tggcttacgc
     7501 tgaggatttt catctttgcc tgatcggtga aagtaacaaa gcctgcaccg taaaatttgg
     7561 gtgtatcttc agctgcacac agggcgctag ccaacttacc gccggtgcat gattcggcgg
     7621 tagttaagcg taattgatcc gcgataagtc tttgactcaa cgcggatgtc aggttttcaa
     7681 tagtgtcggt atctgcaagt tgtacaattt tatttctatt aatgttcact g
//
LOCUS       DAZFBR010000178         7717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFBR010000178 DAZFBR010000000
VERSION     DAZFBR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.178"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..409
                     /locus_tag="ACHJZ2_004580"
     CDS             <1..409
                     /locus_tag="ACHJZ2_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFO4570554.1"
                     /translation="TLVDRKSRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTW
                     DRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHE
                     LDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(417..683)
                     /locus_tag="ACHJZ2_004581"
                     /pseudo
     CDS             complement(417..683)
                     /locus_tag="ACHJZ2_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            842..2026
                     /locus_tag="ACHJZ2_004582"
     CDS             842..2026
                     /locus_tag="ACHJZ2_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4570555.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNLKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDIEDNSTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            2026..2538
                     /locus_tag="ACHJZ2_004583"
     CDS             2026..2538
                     /locus_tag="ACHJZ2_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO4570556.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDDESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            2593..2958
                     /locus_tag="ACHJZ2_004584"
     CDS             2593..2958
                     /locus_tag="ACHJZ2_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4570557.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            2967..3122
                     /locus_tag="ACHJZ2_004585"
     CDS             2967..3122
                     /locus_tag="ACHJZ2_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO4570558.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3109..5912
                     /locus_tag="ACHJZ2_004586"
                     /pseudo
     CDS             3109..5912
                     /locus_tag="ACHJZ2_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5925..6413
                     /locus_tag="ACHJZ2_004587"
     CDS             5925..6413
                     /locus_tag="ACHJZ2_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4570559.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
     gene            6570..7142
                     /locus_tag="ACHJZ2_004588"
     CDS             6570..7142
                     /locus_tag="ACHJZ2_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO4570560.1"
                     /translation="MLSYLMAIHFVLFGNSTNLKNFWKHEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKKAAKKLNSKIINKFGCEIGLGANIGKGLTIPHHA
                     GIVVHFAVDAGENLVLRQNTTIGQIDGDMPSSRVKIGSNVDIGANCCIIGLSRKIGDN
                     VKIGAMSFINKDIPSNCTYITKKSGVVLYK"
     gene            complement(7186..7716)
                     /locus_tag="ACHJZ2_004589"
     CDS             complement(7186..7716)
                     /locus_tag="ACHJZ2_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4570561.1"
                     /translation="MLAGTNEFIGEGDAYIPPHTGLPANSTDIAPPDIPAGFVAVFNS
                     NEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPDGECQKWNGTSWAKDA
                     EAEKLFRVREAEETKNSLMQVASEHIAPLQDAVDLDIATEEEASLLAAWKTYRVLLNR
                     VDTTVAADVEWPVAPQ"
BASE COUNT         1919 a         1816 c         2143 g         1839 t
ORIGIN      
        1 cacacttgta gaccgaaaat cacgttatac gatcatcctc agactcaggg gcaaagattc
       61 tgtctcagta aatcaggctc ttaccgacaa attcctgagt ttaccgtcag aactcagaaa
      121 atcactgaca tgggacagag gaatggaact ggccagacat ctagaattta ctgtcagcac
      181 cggcgttaaa gtttacttct gcgatcctca gagtccttgg cagcggggaa caaatgagaa
      241 cacaaatggg ctaattcggc agtactttcc taaaaagaca tgtcttgccc aatatactca
      301 acatgaacta gatctggttg ctgctcagct aaacaacaga ccgagaaaga cactgaagtt
      361 caaaacaccg aaagagataa ttgaaagggg tgttgcattg acagattgaa tctacaagtg
      421 cctttttctg tcagcggttc tcctttccag ccaatgagaa ccgtcaggcg cgcgccccgt
      481 ggcggtagct gcaactgacc atctgcatca tccagcgtga tggtgagctg gtccgcctca
      541 aatccccggt tgtcggtcag tgacaggctc atcaggcgct ctgccacgcc tgacagcgtt
      601 ttaccctccg cgagaatatc aaaatccggc actttcacgt ggtctgtgcc ctgactgagc
      661 aattgcatgg tggtgtcggt catctgttcc ctccctgtgc ggcatggtcg catgtgcgtg
      721 cggagggggt tactgctttt tgttgtcgcc gtggcgggat gaacggcgca ggggtgagat
      781 tacgcgagtg gtgggtgatg attgttgccg aatcatttaa cggatacaag gggctgaagc
      841 tatgagtgaa actcgttttc atggtgcccg tgttacggaa agtaccgacc tggtaacagc
      901 gattaatgat gttgattcca gtgttatcgg tatcgtggca acggcagatg atgcggacgc
      961 ggagctgttc ccgctgaaca agcccacact gctgacccgc gtcaatgacg tgctgggaaa
     1021 atgcgggact acggggacgc tttatcgtgc gcttaaggct atcgcagacc aggtgagcac
     1081 aaaagtgatc gtcgttcgcg tggctgaaca caaagaagaa ggcggtaaga cgcaggatca
     1141 actggttatc ggtggttctg aatctgacgg cagctatacg gggatgtatg cgctgcttgt
     1201 tgcagagcag gatgaaagca tcggataccg tccgcgtatt ctggccgcgc cggagctgga
     1261 cacggaggcg gtaacaaaat ccctgtgcgt gattgcgggt aaactgcgcg cgtttgtgta
     1321 tgccacatgt catggttgta acacgatggc tgaggcgatt acctaccgcc agaaattcaa
     1381 cgaacgtgag gtgatgctct tatggcctga cttcatcgcc tacaacctga aaagtggcaa
     1441 aaacgaaacg ttccccgcgc ctgcttatgc gtgcggcctt cgtgcgtaca ttgaccatga
     1501 gcagggctgg cacaaatcgc tgtccaacgt tccggttaaa aatgtgcttg ggatgtcgag
     1561 gcatgtgttc tggtcgttgc aggccgaaga cagtgatgcc aacagcctca acaacaaaga
     1621 aatcacgacc attattcgtc gcaacgggtt ccgcttctgg ggcaaccgca caccggaaac
     1681 gaacgcctac atctttgagg tgtatacccg aaccgcacag gtgctggctg attcaattgc
     1741 ggaagcgcag tttgaaacca tcgacagtcc actgacacct gcgaacgtga aggatgttat
     1801 cagtgccatc agggcaaaac tggattcact ggttactgcc gggaaactga ttggcgcgga
     1861 gtgctggtat gacatcgagg ataacagcac cacgaattta cgtcaggggc gtgtgcgtat
     1921 tcgctacaaa tatacgcccg ttcctccgct ggaagacatg gagctttacc agtcgtttac
     1981 tgatgaattc tttggtcccg catttgcggt gctgggaggt gcctgatggc tgtaccaaaa
     2041 catcttcgct tttttacgct gtttgtggat ggtgaaaacg aagtgggtaa ggtgacgtcc
     2101 gtcaccctgc ctaaactgac gcgcaaaacc gacagctacc ggggtggtgg catgatgggt
     2161 gcggtaagta ttgacctcgg cctggacgac tccgcgcttg atgcgagctt tgtcatgggg
     2221 ggcgcagttc gtgagctgtt cctgaagtat ggcggcacga ttgacggcac gctactgcgt
     2281 tttgcggggg aatactacac cgatgatgaa agcgacctgt atgaagtcga aatgcgcgga
     2341 cgtgtgacgg aaattgatat gggggaagcc aaacagggcg aagccacatc acacacttac
     2401 gccattaaaa acacctacta caagctgagt gttaacgatc gcccgttgtg ggagattgac
     2461 ctgctgaact tcatttaccg gaaggacggc aaggacattg tgcccgatcg catccgttcc
     2521 gcgcttgggc ttggctgata agtaatatgc aggcggcgca gtgcgtcgcc tctgactgaa
     2581 aggagtttcc tgatgaaaga gacgaaaaac atcgataccg aaaacacggt agttactgac
     2641 actgtgaaag aaaccagtga gcgtggtgta aaacttaccc aaccaattga gcgaggcggc
     2701 gaaaaaatca cgtatgtgga gatcaccggg gctattgagc aggctggatc tctgcgagat
     2761 ttgtcgctgt ctgatgtgct gaatctgaaa gcggaatcca tgtttacgct gctgtcacgc
     2821 gtgacatcac cgcgactgga tgaagtgacg atcaaaaaaa tggcatcccg tgactttatt
     2881 cagttatgtg tggttgccgt aaattttttg agcggtgcgg actctggcgg gaagaacgaa
     2941 caggcgacgg aagcctgatc acggttgtgt gctttgagca catagaagac tttgtggcag
     3001 atattgccgt tatttttaac tggtcgcccg ccgaaatctt catgatgacg cccggcgaag
     3061 tggttagctg gcgtgagcgg gcggcacttc gcagcgggaa tgcagacaat gaagactctt
     3121 gatatccggg tcgctttcag cgccgttgac aggctgaccc gacctgccga aaacgcacgc
     3181 cgcctgatgg ggcagtttgg tgactccatc cagcgaacgc agggggcgat caaaaatctc
     3241 gagcgtcagg cgcgatcatt tgagcgcgcc cgcgacgctg tcagtaaagc ggatgctggc
     3301 atcgtgaaag cacgacgcca gcttaacgcc cttaatcagt tacaacgcac gggtacagtg
     3361 ctcagcgaaa aacaacaaaa gctgatgcag cagttaagca cccggcttga acgcctgaat
     3421 gaatcgcgca cacgggaaat tcagaaaatg agggaacttg gcggagagct gaaacgccac
     3481 ggcatttccc tgacaggcag cgataacacc attcagcagg ccatcagacg caccgaacaa
     3541 tacaacaacc agcttgaacg cgaacggcag gcgcttgcgc gtgtaacgca ggcgcgtgag
     3601 cggtattcgc acgcgcagga aaccgcggga aaactgaaaa caggtggtgc gctggcaatt
     3661 ggtgcggcag cggcgggcgg ctatgctgcc gggcgttttt tgcagcctgc gatcgggttc
     3721 ggcaaagaga tgtcccgcgt tcaggcactg acgcgaatcg acaaaaacag cccgcagttt
     3781 aaggcgctgc gtgagcaggc gttaaaactt gctgaaacac agtttactgc gagtgatgcc
     3841 gccagtgggc agagctttct ggcaatggct ggttttactc cgcaggccat tcaggccgca
     3901 ttgcccggtg ttcttaatat ggcgctggca ggtggcgtcg aactcggcga gacggctgat
     3961 ataggctcca atatcctcac acagttcaac ctgacagccg atcaaatgga ccgggtgggc
     4021 gatacgctga cagcagcatt tacccggacc aatactgatt tacgcgcgct gggcgaaacc
     4081 atgaagtata ccggtccggt tgccgcaaaa cttggtatca gtcttgaaga agcggcggcc
     4141 atggccggga tgcttgccaa taatggtctt cgcggaagcg atgctggcac ggccatgcgc
     4201 gcaagtctgt cccgccttgc atcaccgcca aaagctgcgg ctgatgcact gaaagagctg
     4261 ggggtgtcag ttgctgaggc cagaggcaaa atgcgcccga tggaggatgt gctgcttgat
     4321 ctctataagg cgacacaaaa atacggacag gtggaccagg tctccttctt caaggacatc
     4381 gccggagaag aggcgttcgt tggtttgcag acgcttgttg cggcggctgg ttcaggagag
     4441 ctgcaaaaac tgaccagaga attgcagggg gcaaggggag aggccgatcg cgtcgcaaaa
     4501 gtaatggctg ataatcttga tggggacctg aaaaatctcg acagcgcatg ggaaggtctt
     4561 cgtattcgca tcagtgatct ggttgacggt ccgctgcgtt ctgtcacgca gtggctcacg
     4621 cgggtgcttg aaaaaatcac ctcgctggcg caggcccatc cggtactgac gcgccagcta
     4681 ctgatagcag gcggtgcgtt gctggcaatg actgcaacga ttggctcgtt gtcgctggtt
     4741 attggggtgc tttacgggaa gctggccacc ctgcgtcttg gttttgacat tcttacccgg
     4801 tcaatgaatg tcgtcagggt gttacctgcg ctatggggaa tggtgacggg ttccgtttct
     4861 ttactgggag gcgttatcgg ggcgttgttc agtccggttg gtcttatcgt ggctgcgctt
     4921 gccggagctg ccgttcttat ctggaaatac tgggatccta tcagggcttt ctttgccggg
     4981 gtgttcagcg ggattatgga aaggctgacc ccgttgcgcg acacctttga acggtttggc
     5041 cctgtttttg acgtaatcgg aagcggttca gccaggtgtt taactggttt aaatcgctgc
     5101 tgtcaccgat ggagtccagc aaggaaacgc tggataaatg taccagtgct ggcgaggtat
     5161 tcggtaacgt tcttggtggt gcgttacagc ttgttctgac gcctgcaaaa atgttgctgg
     5221 atacgctggc gtggatactt gaaaaactcg gtgtgcttcc ggatgaagcg gaaagggcgc
     5281 gcaagaaaat cgaagacgca caacgtgcgg ccattcttca ggacaaggtt gctctgtttc
     5341 agggagacat tgcgaaaatc aatccgccga agtctgcggg aaatagcaat ggcaccggag
     5401 atgataaacc caaagacaac aaacctctca cagacagcaa taccggcacg ctacgcagac
     5461 tcagcaaaat tgctgataac acaggtaagc tggttgatga gacaaaaaaa cgcattggcc
     5521 ccggcgatat tgtctttaag aacctgcccc gcgcacttgc tgttcgtggg gagtggcagg
     5581 agcggaagat tgcgcaggtc agtaagcctg cccccgcaat taatatcaca cccgtggtcc
     5641 cggctccgct gcctccggcg ctggtccctg ttgttgcggc cagctcccgc ccggtggtgg
     5701 aggccatacg atcgccagtg gcatcagttc ctgcaacttc ccgtaaccgg gagcctgttg
     5761 cctccggatt tggcggtgaa attcatgttc atctgcataa cgttgttacg cagaatcccc
     5821 gcgaactggc gaaactggtc ggcgaaatgg tcagggcaga aatggaacgg cgcgcccgtg
     5881 ccaggcgtgg cagtttttac gataaagatt gaggagtcat ggccatgatg atgatctacg
     5941 gcatgtttgt ttttgagctg cgcacgttgc cgcatcagca gttacagcaa aacaaaagct
     6001 ggcgacatgt gaaaaatgaa cgcgttaacc gttcagcaag ctggcagtat atcggtgcag
     6061 gtgatgatcg catcgttctt tctggtgttc tttatcctga gattactggt ggcgaagtgt
     6121 cgctgtcgtt gttgaccacg caggcatata caggacgtcc ttggcctttg attgatggcg
     6181 tcgggcagat ttacggcatg tatgtcctga ccggaacgaa tacgacccgc tccgagtttg
     6241 atcgctacgg taaggcgaaa aagatagaat tttcactgac tcttgaacgc tgtgatgagg
     6301 atttgcggga gcgcctgcaa tcctcatcgt tcagcgatat gctgtccggc ttcaaagata
     6361 aggtgacatc atcccttaat agcgcggcca gctcagttaa ggggctgctc tgatttaacg
     6421 tatgtcgcca atttcctgat gaaggtgact ggcgacttgc tgttgtatgt ccttctcaga
     6481 aaattgtttt tgaataacaa ataacaggat tttataatct cttaacctta taacatgtgt
     6541 ggtctgaaat aataattaag gagattatcg tgctatctta cttaatggca attcactttg
     6601 ttttatttgg aaactctact aatttaaaaa acttctggaa acatgaagta attcggcgga
     6661 aacgtatgga tatctggagg cttttaagag agaaaaaaca gcgtaaccgg aatttccttt
     6721 tctggtggcg gttggctaac gaaatgtata ttaatggtaa taaattacat aagaaagcag
     6781 ccaaaaagtt aaacagtaaa ataattaaca agtttggttg tgaaatagga ttgggcgcaa
     6841 atattggaaa agggttgaca attccccatc atgctggaat tgttgttcat tttgctgttg
     6901 atgcgggtga aaatctggta ttacgacaga atactaccat tggacagata gatggtgata
     6961 tgcctagttc aagagtaaaa attggtagta acgttgatat cggagctaat tgttgcatca
     7021 ttggattatc acgtaaaatt ggggataatg taaaaatagg tgcaatgtct tttataaata
     7081 aagatatacc atcaaactgc acatatataa ctaaaaagag tggtgttgta ttgtataaat
     7141 agagtacata aagccatcga tatttctgtc gatggctttt tctttttatt gtggggcgac
     7201 tggccactca acatccgctg ctactgttgt atcaacacga ttcaacaata cccgatatgt
     7261 cttccatgca gccagtaacg atgcctcttc ctccgtcgca atatccaaat ctacggcatc
     7321 ctgaagtggc gcaatatgct cactggctac ctgcatcagg ctgttttttg tttcttctgc
     7381 ctcccgtacc cgaaacagtt tttctgcttc tgcatctttc gcccaggatg tgccgttcca
     7441 cttctgacac tctccatccg gcgacaacca ggtaacattt tccggtaacg gaccgagttc
     7501 agaaataaat aacgcgtcgc cggaagccac gtcataaacc gttttacccc gatgatcttc
     7561 aacgagatgc cacgatgcct cattactgtt gaaaacagcc acgaagccag ccggaatatc
     7621 tggcggtgca atatcggtac tgtttgctgg cagacctgta tgaggcggaa tgtatgcgtc
     7681 accttcacca ataaattcat tagttccggc cagcaga
//
LOCUS       DAZFBR010000179         7450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFBR010000179 DAZFBR010000000
VERSION     DAZFBR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.179"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..689)
                     /locus_tag="ACHJZ2_004590"
     CDS             complement(<1..689)
                     /locus_tag="ACHJZ2_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO4570562.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRR"
     gene            complement(700..1701)
                     /gene="ltaE"
                     /locus_tag="ACHJZ2_004591"
     CDS             complement(700..1701)
                     /gene="ltaE"
                     /locus_tag="ACHJZ2_004591"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO4570563.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1738..3456)
                     /gene="poxB"
                     /locus_tag="ACHJZ2_004592"
     CDS             complement(1738..3456)
                     /gene="poxB"
                     /locus_tag="ACHJZ2_004592"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO4570564.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAASAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3589..4557)
                     /gene="hcr"
                     /locus_tag="ACHJZ2_004593"
     CDS             complement(3589..4557)
                     /gene="hcr"
                     /locus_tag="ACHJZ2_004593"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO4570565.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4569..6221)
                     /gene="hcp"
                     /locus_tag="ACHJZ2_004594"
                     /gene_synonym="priS"
     CDS             complement(4569..6221)
                     /gene="hcp"
                     /locus_tag="ACHJZ2_004594"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO4570566.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6365..7264)
                     /gene="lysO"
                     /locus_tag="ACHJZ2_004595"
     CDS             complement(6365..7264)
                     /gene="lysO"
                     /locus_tag="ACHJZ2_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO4570567.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         1791 a         2016 c         1968 g         1675 t
ORIGIN      
        1 cggcgcttac cgctcaccgc cataaaatgt tcaaactgtt gctgataact gagcacctct
       61 ggtccggcgg cttcgaagat gcggtgttcg ttggctggat ggtctaacag cgccaccaga
      121 tagtgcagca agttttccag cgcgatgggc gtggtgcgtg aacgtaccca gcgtggcggc
      181 gttaacactg gcaggttgta gaccatatcg cgcatgactt cgaacgctgc tgaacctgca
      241 ccaacgatta ttccggcacg caattcggtc actggtacat tcgcttcacg aagaatgtcc
      301 gccgtagcct gacgcgcgcg cagatggtcc gactgctcat gtggcggggc ctgcaacgaa
      361 ctgagaaaga ttaattgctt aactggtact tcacgtagcg catcgcggaa gttgagagcc
      421 acctggcgct cctgagcgat aaaatcgccg ccttcgccca tgctatgcac cagaaaatag
      481 accgtatcga tatcctgtaa cagggccgga aggttatccg gccagctgag atcgacttta
      541 tggcaactga cgtttgccag ttgcagcttt gcaagcctgt cgacatgacg tgccgccgcc
      601 aggatctgat gcccttgctg gctgagtgtg cgcaccagat gctgaccaat gtagccactg
      661 gcaccaagaa ctaaaatgcg ttgcggcacg tctccctcct taacgcgcca ggaatgcgcg
      721 ccagtgggcg gcgacttctg ccagttgttc gcgcgagacg tcaagatggg tcaccaggcg
      781 gacaatcggc gaggcgttaa tcagtacgtt tctcgctttc atgtattcgc ctaacgcggc
      841 agcattttct tcgccgacgc gaacaaacag catattagtg tcctgacgca tcacatccgc
      901 gcctgcttca cgcagttgct ccgccatcca ggcggcgttg tcgtgatctt cctgcaaccg
      961 tgcgacgtta tttttcagcg catacatccc ggcggcagcc agaatgccgg actggcgcat
     1021 cccgccacct gtcattttcc gccagcgaat ggcacgttta atgtaatcac gattaccgac
     1081 gagtaatgaa ccgactggcg tcccaagacc tttcgacagg caaatggtga acgaatcaca
     1141 atattgcgtg agctctttca gttcgctgcc gtaagccacc acggcattaa agatgcgcgc
     1201 accgtcaaca tgcagcgcca gattgcgctc gcgggtaaat tcccatgcat ctttaaggta
     1261 ttcacgcggc aacactttgc cgttgtgggt gttttccaga ctgagtaatt tggtgcgggc
     1321 gaaatggata tcgtcgggtt tgattttcat cgccacttta tccagcggta gcgtgccgtc
     1381 ggcagccgcg tcgatgggtt gcggttgaat actgcccagc accgccgcgc caccggcttc
     1441 aaacagatag ttatgcgcgg cctgaccgac aatatactct tcgccgcgtt cgcagtgact
     1501 gagcagagcg accaggttgg cctgagtgcc ggtaggcaga aaaatggcgg cttctttacc
     1561 ggaaagctct gctgcatagt cctgcagagc attaacggta gggtcgtctc cgtaaacgtc
     1621 gtccccaacc ggggcggcca tcatcgcttc gagcatggcg cggctcggtc gggttacggt
     1681 atcactgcgt aaatcaatca tggcatgtcc ttattatgac gggaaatgcc acccttttta
     1741 ccttagccag ttcgttttcg ccagttcgat cacttcatca ccgcgtccgc tgatgattgc
     1801 gcgcagcata tacaggctga aacctttggc ctgttcgagt ttgatctgcg gtggaatggc
     1861 taactcttct ttggcgacca ccacatccac caataccgga ccgtcgatgg agaaggcgcg
     1921 ttgcagggct tcatcgattt cagaggcttt ttctacacga atacccgtaa ttccgcacgc
     1981 ttcggcaatg cgggcaaagt tggtgtcgtg cagctcagta ccgtctgtca ggtagcctcc
     2041 ggctttcatc tccatcgcca caaagcccag cacgctgtta ttaaagacga taattttcac
     2101 cggcagtttc atctgcacta ccgagaggaa atcgcccatc aacatgctaa aaccgccatc
     2161 gccgcacatg gcgaccacct gacgttccgg ctcggttgcc tgcgcgccca gcgcctgcgg
     2221 catggcgtta gccatcgaac cgtggttaaa cgaacctaac aggcgacgct tgccgttcat
     2281 tttcagataa cgcgccgccc acaccgttgg cgtgccgacg tcacaggtaa agatggcgtc
     2341 atcggcggca aaatgactaa tttgctgcgc cagatattgc gggtgaatgg ctttctcgct
     2401 cggtttagct aaatcgtcca gccctttgcg ggcatcgcgg taatcttcca gcgctttatc
     2461 cagaaacttg cgatcggctt tttcttccac caatggcagc aatgcacgca gggtcgactt
     2521 gatatcgccg accagcgcca tatccacctt gctgtgtgcg ccgatgctgg ctgggttgat
     2581 atcaatctga ataattttgg catcggtcgg gtagaacgca cggtagggga actgagtacc
     2641 gagtagcact aacgtgtcgg cgttcatcat ggtatggaaa cctgacgaga atccgattaa
     2701 cccggtcatt ccaacatcat acggattatc gtattcgaca tgctctttac cgcgtagggc
     2761 atgaacaata ggtgctttaa ttttcccggc aaactcaact aactctttat gcgcccccgc
     2821 gcagccgctg ccacacatca gagcgatatt gctggaataa cgcagcagtt gcgccagttt
     2881 gcgtaactct tcttcttccg gcgtcacgac tggctgtggc gcatgatacc agtgggtagt
     2941 tgccccttct ggcgcaggtt ttaacgccac gtcgcccggt aacacgacaa cggaaacgcc
     3001 acggttaagc accgctttgc gcatggcaat cgccagcact tgtgggatct gctccgggct
     3061 ggaaaccagc tcgcaatagt gactacattc gcggaatagc tcttgtgggt gggtttcctg
     3121 gaaatagccg ctgccaattt cgctggaagg aatatgagcg gcaatcgcca gtaccggaac
     3181 gtgattgcgg tggcaatcga acaggccgtt gattaagtgc aggttgccgg ggccgcacga
     3241 tccagcacag accgccagtt ctcctgaaag ttgtgcttca gcgctagcgg caaacgccgc
     3301 cacttcttca tggcgggtgg acatccactc gatggtgccc atgcgattaa gactgtcgct
     3361 aagaccgttc agagagtcgc ccgtgactcc ccagatgcgt ttcacgcctg ccgattcgag
     3421 tgttttggcg atataagctg caaccgtttg tttcatggtt ctccatctcc tgaatgtgat
     3481 aacggtaaca agtttagttc atctgacgga gggggaaggg atgggagaga aaggaggcac
     3541 taacggttaa atagcccgat gaaaggaata tcatcgggca taaggcgatt atgcgagaac
     3601 caaatccccc tgcggatggc aggagcaggc cagtacgtaa ccttcagcga tttcggcgtc
     3661 ggtcagcgtc attgtactgc tcaccgtata ttcaccggaa accactttcg tcttacagca
     3721 gccgcaaaca cccgcgcggc aggcggcgac aaccggaacg ttattgcttt ccagcgcctc
     3781 cagcagcgtg gtgccaacag gagcgtaaaa ttcttgcgcc ggttgcagtt tggtgaattt
     3841 cagaccgctg gtcgccgctt ccgctactgg ggtgaagaat ttctctttaa agaaacgcgt
     3901 cacgccgagt gctttcactt cctgctctac ccaatccata tacggagccg ggccgcaggt
     3961 catcacggta cgtgaagcta agtcaggaac gcgagtcagg agttcgcgag tgagacgacc
     4021 agcgataaag ccttcggtaa cgttattttc cgccaccagc gttaccggat agttacgcca
     4081 ctcatcggcg aaaataacat cctgcggcgt acgcacgttg tagatcaccc gcacatcggc
     4141 ctgtggacgg ttcttcgcca gccagcgacg catcgacata atcggcgtga cgccgcagcc
     4201 tgcagccagc aacaggaatt tatcttctgc tttatcgtcg caggtaaatt cccccatcgc
     4261 gtctgaaagc cagagataat caccgcgttt tacatcgcgg gtcagccact gggagccgac
     4321 accgtcatca atccgccgca cggtcagggt gatatattca ctcacgcctg gcgtggagga
     4381 aatggtgtaa gcacgcagcg tttccgctga gttacgcacg ctgaccagtg catattgccc
     4441 ggcgcggtat gggtagtaat cgtggcaaat cagggaaatc gtccacacat ccggcgtttc
     4501 ttgcttaatg tgatgaacct gcatccgcca cgggcattga ttcgttggca tcgtcatcga
     4561 caaactcctt acgcgctcaa cagttgcttc atgtcttctt caacagtggt gatagaacgc
     4621 aggccgaatt tctcgttcag caccgccagc agatctggtg tcaggaaacc aggtgcagtc
     4681 gggccggtga cgatattttt cacgcccaga gaaagcagcg tcagcagaat gacaatcgct
     4741 ttctgttcaa accaggagag caccagcgac agcggcagat cgttgacacc gcagcccagt
     4801 ttttctgcca gcgtaaccgc cagaataatt gctgagtaag catcgttaca ctgaccggca
     4861 tccaccagac gaggcagacc ttcgatatcg ccaaactcaa gtttgttaaa gcgatattta
     4921 ccacaggcga gagtcaggat caggcagtca tctggcacgc tggtggcgaa atcggtgaag
     4981 tagtgacgtt cgccgcgtgc gccgtcacag ccaccaagca ggaagatatg acgcagtttt
     5041 tcacggctca ccagatcaat tagcgtatcc gctgcgccaa gcagcgtctg gcggccaaag
     5101 cccacggtga tcaggtgcgg aatttcgctg tatgggaagc ctgccatttg ttgcgcctgg
     5161 gcaatgaccg cagagaaatc ttcgccatcc agatgacgca cgccaggcca gccaacgatg
     5221 ctgcgggtcc agatacgatc gtcataagcg cctacggttg ggtcgatgat gcagttcgag
     5281 gtcatcacga tggggccagg gaaacgagcg aactccactt gctgattctg ccagccgctg
     5341 ccgtagttac cgaccagatg cttgaattta cgcagctccg ggtagccatg cgcaggcagc
     5401 atttcgccgt gggtgtagac attaacgccc gtgccttcgg tctgttccag caggttgtag
     5461 agatctttga gatcgtgacc ggaaatcagg atgcatttac ccgccgtcgc tttgacgttg
     5521 acctgggttg gcgtcgggtg accgtattta ccggtttcgc ctgcatccag aatgctcatc
     5581 actttgaagt tcatctggcc gatttccatt gaacactcaa gaagcgcgtt catatcggca
     5641 ggccaggtcc ccagccacgc catgatttta tgatactggg cataaatatc gttgtcgtat
     5701 tgaccgagaa cgtgtgcgtg ttccatatag gccgccgcac ctttcaggcc atacaggcac
     5761 agcagacgca ggccgagaat gttttcgcca atcgccgctt tatctttgtt aggggtaaat
     5821 tctgctgcct gacgttgcag ttcgccgaga tcgtcgctca ccagttgcag gtcagccatc
     5881 gggttatcga cgcgggcgtt ggcatctaca gccaggcatt gtgctttcag cgcctcgcgc
     5941 aaggcaatcg cttcacgagc gtagccgaca atacgcggag aatcgaagtt aacgttggtc
     6001 agggttgaga aaaaggcacg tggcgcgaag ctgtcgacat cgtggttgat gatgccgtat
     6061 tcacgcgctt ttaccgccca ggcagaaagc ccttgcagcg ccgcgatgag taaatcctga
     6121 aggtcagaag tttccgccgt tttaccacac atcccctgcg cgtatgagca gccgtttcct
     6181 gccggagtac ggatagtttg ttcacattgc acacaaaaca tgatcacacc ttttaaagtt
     6241 atatttaata tacatgttta aggttaagac gcttaacgcg gggataaaag ggatttttca
     6301 tgcaacttta agggagattg atttagcgca attttggcgg cagggatcta ccgccagaga
     6361 ggtattacgc ggagaaaaag gcgatgagga tcggcactaa caggctaaga ataaaaccgt
     6421 gaacaattgc cgccgggacc atatccagcc cgccagtacg ttgaagaacg ggcagggtga
     6481 aatccatcga cgtcgcaccg cataagccca gtgcggtaga gcggctgcgg cgaatcagcc
     6541 cagggatcaa cataattgca atcagctcac gggccagatc attaaaaaac gccgcgctcc
     6601 cgattaccgg accaaaagat tcggtcaata aaataccgga aagagaatac cagccgaaac
     6661 cagaggccat tgccagcgcg gtattgatgg gtagatcaag aataaaggcg ttaattaaac
     6721 caccaattaa tgaactggca accaccacca cggcgacaat cattccccgg cgattaagga
     6781 caatctgctt taaggtcatg ccattattgc gcaactgaat accgacgagg aaaagtagca
     6841 gaattaacgt gtattcactg gcttcggtcg cgtgttgtaa gaaagccagt ccactcaggc
     6901 caatggcaaa accaatcact actacgccgc acagttttag cgactcaagc gccatcgcaa
     6961 tacgcgacgg gagtttttct tgctgatggt ggttgcgcca cggcagaccg cgctccagcc
     7021 acataagggc ggcaatatta cacagtaaaa taacggtaat actgacggca gaataatgca
     7081 gaatcgccaa caggttactg gcgaggttat cgagaaacgc cagactgata cccataaaaa
     7141 agagaataag gtaaaccatc cagcttaata gctgattaat aacttttaac gcagcttgtt
     7201 ggcgaagcgg aatgaggtaa cccacaatca ggggaaccag aatgattaac aacccagaaa
     7261 acatgaaaaa ccagcccttg caaagaaaga agtcgaaatg cgcgatgaca cactactgaa
     7321 agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt atgcatcaga gggggatttt
     7381 taattctata tgagtgttta gcattgttgc gctcctttca tgtgcaatgc catataaagc
     7441 cactggcgca
//
LOCUS       DAZFBR010000180         7231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFBR010000180 DAZFBR010000000
VERSION     DAZFBR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.180"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            55..588
                     /locus_tag="ACHJZ2_004596"
     CDS             55..588
                     /locus_tag="ACHJZ2_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4570568.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYTRTSGNP
                     VTKGSVESTVTIKISAD"
     gene            638..1321
                     /locus_tag="ACHJZ2_004597"
     CDS             638..1321
                     /locus_tag="ACHJZ2_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4570569.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVTFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLGIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSHNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            1334..3784
                     /locus_tag="ACHJZ2_004598"
     CDS             1334..3784
                     /locus_tag="ACHJZ2_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4570570.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMKGEKVSEYHF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDKAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGTWQSNTLYLEHGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYIIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     ISNFDFIAGRSKIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDITHEGTSYQVAYNKYLVQTATRFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            3797..4757
                     /locus_tag="ACHJZ2_004599"
                     /pseudo
     CDS             3797..4757
                     /locus_tag="ACHJZ2_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(4795..5694)
                     /gene="nhaR"
                     /locus_tag="ACHJZ2_004600"
     CDS             complement(4795..5694)
                     /gene="nhaR"
                     /locus_tag="ACHJZ2_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO4570571.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(5760..6926)
                     /gene="nhaA"
                     /locus_tag="ACHJZ2_004601"
     CDS             complement(5760..6926)
                     /gene="nhaA"
                     /locus_tag="ACHJZ2_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO4570572.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         2253 a         1578 c         1487 g         1913 t
ORIGIN      
        1 ataatcgggt ttgaaaacga gtgaccttcc ataccagtta attaaggatg atttatgaaa
       61 aagtatatta ttcccttcgt tgcgacaacg ttgatgttta gcgcaacaac cttcgccaag
      121 gatgtcgatc aaggaacact aaccattaga ggagaagttg ttgatacaac gtgttacttt
      181 gttaatggtg attcgacaac agatatcgtt gtcgaagatg ttgtaaccag tgcaatgaat
      241 caacttatca ataatgaaat ctatacttta aaaaccggtt caacctctca tgacttagaa
      301 attcagtgtg acggaaatac agccccaagt atcgaagtac tagcatcaga gtttgatgct
      361 aataacttta cgctgaataa aggaactgct gagaacattg gctttgccat tttcctcgat
      421 gatgggactt tgaccagaat gagaccaggg ttaaaaacac cgctgaaaga taatggctct
      481 ggtcaatatt ttctcaattt aaatgcacaa tatacccgca caagtggcaa tcctgttaca
      541 aaaggctccg tagagtcaac cgtaacgatt aaaatcagtg cagattaacg ttttttgccg
      601 gtagctctgc taccggtatt atttacggag tgattacgtg agaataataa cagcattact
      661 gatgtcattt tttattttac ctgttcatgc cggtgtggtc atctatggta cgcgcattat
      721 ttatccggaa aaaaacagag aagttctcgt acagttaatg aatcaaagta aaaatgcctc
      781 tttgattcag gcatggatcg atgatgggaa caccactatc gcgccagaaa aaattcaggt
      841 cacatttatt ctcacaccgc cagtaagtcg tgtagcaggt ggttctggcc aacaacttaa
      901 aataagaaaa atgccaaaca atttgcctca caataaagaa agtttatttt atctaaatgt
      961 tctgggtatc cctccaaaca atccacaaaa tgcagggaaa aacaaaatta aacttgcatt
     1021 gcaaaaccgg ataaagttac tttggcgtcc aagcggaata gctccggtag ataaaaaaag
     1081 cctttctcaa ttaaatatca aaaagaaaaa taacgccatc tcaataaaca acgaaacagc
     1141 caactggata acggttacaa ctatcaaagc acaaaacgtg aaagttaata atgaaagtat
     1201 cttactgcct cccttttcgc ataacgacat tacattaaaa aataaccacg catcagaata
     1261 tgaactaacg gtagttgatg attacgggaa taacatccat agtaaaatcg ccgcgagata
     1321 acaggaaaaa gtcatgttaa ggacgacacg atgggtcgct gctattattt ttttgtattc
     1381 ttttccgggt tatgctgaag aaacttttga tacgcacttt atgatcggcg gcatgaaagg
     1441 agaaaaagtt tccgaatatc atttcgataa taaacaaccg cttccaggaa actacgaact
     1501 tgatttttat gtaaataacc agtggcgtgg aaaacaagat atcacgatcc cagagtcacc
     1561 agtcaaacca tgtctgccta aggtgctact aaccaaactg ggggtaaaga ccggtaacct
     1621 caatacagaa gataattgta ttttactgga taaagcggtt catggtggac aatatcagtg
     1681 ggatatcagt gaacaccgac taaatttaac agtaccacag gcgtatatca atgaacttga
     1741 aagaggttac gttcccccgg aaagttggga tcgcggcatt gatgcttttt atacctccta
     1801 taatttaagt cagtatcgct cttatgacag caataataac agtaatacag ccagttatgg
     1861 ccgatttaac agtggattaa atttatttag ttggcagtta cactctgatg ccagctatag
     1921 caaacccgat gatatgaaag gcacatggca aagtaatact ctttacctgg aacatggctg
     1981 gtcgcagata ttaagcacag ttcagatagg agaaaattac acatcctcac tcatattcga
     2041 ctctctgaga tttagcggaa tacgcttatt tcgcgatatg caaatgctac ctgattctat
     2101 gcagagcttt acacctttag tacaaggtgt tgcgcaaagt aatgccttga taacggtttc
     2161 gcaaaatggc tacatcattt accaaaaaga ggttcccccg ggaccattta ctatcgcaga
     2221 tttgcaactc tcgggcagcg gctctgatct cgatgtcagc ataaaggaag cagatggcag
     2281 tgttcgttcc tttttggtgc cctactcttc cgtgcctaac atgctgcaac ctggtatttc
     2341 aaatttcgat tttatcgccg gacgcagtaa gatatatggt gtaaagaatc aggaggactt
     2401 tctcgaggca aattatatat atggcctcaa caatcttttg acactttatg gcggtacgat
     2461 tttatccgat aactataatg ctataactct gggaaatggt tggaatacac cgctgggtgc
     2521 catctctttt gacgctacac gatcgagtag taagttaaat aatgatataa cacacgaagg
     2581 tacgagctat caggtcgcct ataataaata tctagtacaa acggccactc gatttagcgt
     2641 ggccgcctgg cgttatgctt cgcaggatta caggacattc agcgaccatc tttacgaaaa
     2701 tgataaaata aatcatcaga gtgactatga cgatttttat gatattggca gaaaaaatag
     2761 cctttctgcc aatatcatgc aacctttatc caataatctg ggaaatgtat cattaagtgc
     2821 gctttggcgg aattactggg ggcgaagtgg aaatgctaaa gattaccaat tcagttattc
     2881 caatagctgg caacgcatca gttatacttt ctctgccagc caatcctatg atgaaaacga
     2941 caaagaagag gagcgtttta atctgtttat ctccattcct ttctactggg gggatgatat
     3001 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
     3061 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
     3121 tatatatgtt aatcagcaac aacaaaataa tgatacatcg cttggtacga atttaagctg
     3181 gagaactccc atcgccacaa tagatggcag ctatagccat tctaaaaacg cctggcaaag
     3241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
     3301 gctatccgat acatttgcaa ttctggatgc gcctgggtta gaaggcgcgc atattaatgg
     3361 acaaaaatac aaccgaacaa acagcaaagg ccaggttgtt tacgacctga tgatacctca
     3421 tcgtgaaaac catctggtac ttgatacagc aaacagtgaa agtgaaacag aattgcaggg
     3481 caatcgtcaa attattgcgc cttaccgtgg agcagtttct tatgtgcagt ttacaactga
     3541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacttttgg
     3601 ctatgacgta ctggatctcc aggaaaacaa tattggagtc gttggccagg gtagtcgcct
     3661 ttttattcgc gtagatgaaa ttccaactgg cataaaagtt gctctcaatg atgaacagaa
     3721 tttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
     3781 gtaaaaggag tttactatga agtggctttt attaatcaca ttgagtctat attcttttat
     3841 tgtgcaaagt gctccctgtg gattgaccaa tgtcggggaa caacgtggaa catatatact
     3901 caaaccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaata ctgggaatat
     3961 cattgagtgt gcagacttgc aagagacgac caacgccatt aaagttcatt ttttaacaca
     4021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     4081 tgacggtaaa aaaaatagcg cttccaggga agtcatctta taatttagaa gaaatgctat
     4141 ctgcgataaa tttcaccatt ccatactcta tcgcatctgt atcagatagt tccaacaccg
     4201 cagatatttt tccaggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta
     4261 cagggaataa tactaattgt aatagcgtcc gctttaattt taatttaaat actattttac
     4321 aggtaaattt aatgacctgt ggttttgaag atcaatccat cgacactgga cgatttgtac
     4381 tgtctcgtat caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg
     4441 gcgagcaagg tgaattagtt tttgaaccca gtgacgttga atattacttt gaaccagtga
     4501 cgatacagga gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg
     4561 cgggtcaaat aggctttcaa ctctccaatg atggtacgaa tgaaatccag tatggtaaaa
     4621 gcaattacta ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc
     4681 gcccgcgtac atatggaaac aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg
     4741 tggtaatgta caactaatct atctccccca ccttaacttt gggggagctg ccgattaacg
     4801 caccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     4861 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     4921 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     4981 gattgcattg tgcatcgcac caaaagcttt catcaaagcg gcatcatcaa actcgccaag
     5041 gatttctacg tttaatccct gggagttaaa ccagttaagt aatttgcgcc ctaacattga
     5101 acgtcgccca ggaatcaaaa gccgccgttc ttcaagacaa gctgggaacg gtttttccgg
     5161 cggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     5221 ttcctgctgc gtagaatcta tcggacagtc ggaaatgatc atatccagtt tatgctggct
     5281 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tcggctcgcc
     5341 ttctactact gcagcattca gcacgctact gaccagacgt ttggaaagta catcagccac
     5401 gccaacgtca aacaataaat tggattcttt gcgatagttc acgatatcca gcatttcctg
     5461 gctcaaggta aacattttat cggcatagcg atagaccagc tcccccagct cgctgggctc
     5521 gagaccacgt cccttgcgct taaataattt gccttgcagg cgctcttcca gcgcccgaat
     5581 ctgtccggta atggtttgtg gtgttaaata aagcgcctcc gctgcgccaa ccacagaacc
     5641 ttctttataa acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     5701 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     5761 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     5821 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     5881 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     5941 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     6001 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     6061 ggcagaatgg aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc agcattagca
     6121 aatgcaaaca gtggtaaaat cagatacgcc acccacgggt gcaacacatg ctccagtcgc
     6181 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     6241 gccagggttg cgtgaacccc cgatttcaac actgcagtcc acaacactac gccaaccaga
     6301 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac agcaattgct
     6361 acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     6421 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagcgctaac
     6481 ggaacacgac ttcccaatag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     6541 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     6601 gccggcacaa tcatcccacc aatagcagcg ataactggaa atgcggcctg gcgtaagctg
     6661 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     6721 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     6781 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctgttg
     6841 gccatcatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     6901 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     6961 tactatttta ccccgcacga atcatcgtta aaatcagatt atagtgtagg aataattcgt
     7021 tttttacgat ctatagaaga atttgcgcca tcagcggtga gatgcgtttt tgacggctgt
     7081 aagcgctgac gggtgaccct aagccctgat aatcatagct taaggtgagg atttctgagg
     7141 gattaattag gaaaagtaat aattaatttg ttgtcagtcg gcgatgtttg ttaggccaat
     7201 atttcaaaaa taatccagct tgtcgtcaat a
//
LOCUS       DAZFBR010000181         7204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFBR010000181 DAZFBR010000000
VERSION     DAZFBR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.181"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(36..1694)
                     /gene="sgrR"
                     /locus_tag="ACHJZ2_004602"
     CDS             complement(36..1694)
                     /gene="sgrR"
                     /locus_tag="ACHJZ2_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO4570573.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            1783..1914
                     /gene="sgrT"
                     /locus_tag="ACHJZ2_004603"
     CDS             1783..1914
                     /gene="sgrT"
                     /locus_tag="ACHJZ2_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO4570574.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(2022..2627)
                     /gene="leuD"
                     /locus_tag="ACHJZ2_004604"
     CDS             complement(2022..2627)
                     /gene="leuD"
                     /locus_tag="ACHJZ2_004604"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO4570575.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(2638..4038)
                     /gene="leuC"
                     /locus_tag="ACHJZ2_004605"
     CDS             complement(2638..4038)
                     /gene="leuC"
                     /locus_tag="ACHJZ2_004605"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO4570576.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(4041..5132)
                     /gene="leuB"
                     /locus_tag="ACHJZ2_004606"
     CDS             complement(4041..5132)
                     /gene="leuB"
                     /locus_tag="ACHJZ2_004606"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO4570577.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(5132..6703)
                     /gene="leuA"
                     /locus_tag="ACHJZ2_004607"
     CDS             complement(5132..6703)
                     /gene="leuA"
                     /locus_tag="ACHJZ2_004607"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO4570578.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(6796..6882)
                     /gene="leuL"
                     /locus_tag="ACHJZ2_004608"
     CDS             complement(6796..6882)
                     /gene="leuL"
                     /locus_tag="ACHJZ2_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO4570579.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         1589 a         1923 c         1907 g         1785 t
ORIGIN      
        1 taaaaaatgc gctcatgacc gccaaaaggc gcaagctact ctggatccgg cggcgcaaac
       61 cacgctgatt taaaatcaaa ccagccgagg gtgttcatgc gcaggccgcg catactgcgt
      121 tgtccctgaa tgatcagcca gtggtggata agtggcacca ttgctttgct ggcgaccagt
      181 tgttggcacc agttcgccag gttcatttcg ccattgcgcc agcgggcggc gtcgacttgc
      241 cagtcgattg gaatacagtg ttggagcagc ggtacctcgc acaggtgcgc gaacagcgaa
      301 aaatccagcg gcagcgtaaa gttggcgctg ttaagccaga tatcgctctc gatctctcct
      361 tcatgccact gatcgtagct gatctctttg atttccagtg tgacctggtg gcttgccaga
      421 atctgctgca tgatcccggc aatcacccga tgctcactgt gatcctgata aaaggtcagg
      481 gtgaggcttt ccagaccagc cggtttttcg ctcgttatgg tgcgggcatg atgccaacgg
      541 gggagcagtc cataagccgg gaaccacagt tgctggtact gttcctcagc gaaatagacc
      601 agattagttg gagaaagcac atagcttacc cagtccctga cttgctgatt cgccccgcga
      661 tgggtgcggc tatcgaacag taaatagtag caaccttcct ccaggcggct ttcaatctct
      721 ttttcctcgc cctgtggacc ttttagcatc agccctccgg ctggctcgtc ggcaatttcc
      781 ggcagaaccc agacgttaac ttcatcgatt aatgcccggt aaccgaagaa gtcatcgaat
      841 gcctgaattt tcagttgatt ggtgctgttg cgaatcaccg catacggacc ggtgccgatg
      901 ggatggctgg caaagttact gagggtttcc cattcgcgcg gcaggatcat cgccggaact
      961 tgtcccagca gtaacggcaa ccagcgatcc ggctgcgtga ggtggatatc cagcgtccag
     1021 ggcgtcggcg acacaatgtc agcaatatgc gaatagagcg gcagcgtatt gattcgtttt
     1081 aaagaggcga tcacgtcgtc catttccagt tcacgaccat gatgaaaatg gactcctgga
     1141 cgcaaaaaga aacgccagtg aagcggtgat atttgctgcc agtggtgggc gatgtctgct
     1201 tccagttccc cattttcctc atttatgcgc gttagcgaac tgaagatttg ccgggcgata
     1261 tgggtttcgg aacggcgcaa tgcgctgcca ggtagcagat tacgcaacgg acgatagtag
     1321 agcacgcgca ggatgtgccg cccctggcgg aagctgcggc ccagatgaga aaccagcatt
     1381 tgcctcacag tcgctttgtc gccaactaac tgcactagtt ggtcgatacg gtcctgctcc
     1441 agcaggtctt ccgcccgctg ttgctgaagc gccagcccgg tatagaggaa tgtcagacgc
     1501 gagcgtttac cacgcccgac ttccgcttcc cacgtcagcc agccgcgatc ctgcatggtg
     1561 ttgagcaggg tacgcatatg acgacgcgag cagctcaata acgctgccag ttcgttgagc
     1621 gttgtgtcct gcgatttacc ctcgcagcat tgccacaggc ggatgaactg ttgttgcaga
     1681 cgagcagatg gcataaaagg ggaactcctg cgcaaaagac agcaatttta ttttccctat
     1741 attaagtcaa taattcctaa cgatgaagca aggaggtgcc ccattcgtca gttttatcag
     1801 cactatttta ccgcgacagc gaagttgtgc tggttgcgtt ggttaagcgt cccacaacga
     1861 ttaaccatgc ttgaagaact gatgcagtgg gaggggagtc attctgacta ctgatttgcg
     1921 gacatcatgt gtgactgagt attggtgtta atcgccacgc cagcagtgat tatctgctgg
     1981 cttttttctt tttggcgatt gagtatcgga cggtgtacaa actaacgcat aaacgcgggt
     2041 tgcttcgctt cataagccgc aatggcgtcg tcgtgctgca aggtaagccc aatgctgtcc
     2101 agaccgttca tcatgcagtg gcggcggaag gcatcaatgg taaagcgata ggttttctca
     2161 cccgctttca cctcttgcgc ttccaggtct acatcaaaat gaatccccgg attggctttc
     2221 accagcgcaa acagctcatc cacttctgcg tcgcttaatt tcaccggcag cagctggttg
     2281 ttaaagctat tgccgtagaa gatgtcagca aaactcggcg caatcaccac cttaaaaccg
     2341 tagtcggtca atgcccaggg cgcgtgctca cgcgaggagc cacagccgaa gttttctcgt
     2401 gccagcaaaa tggaagcgcc ctgatactgc gagaagttca gcacgaagtc cgggtttggc
     2461 tgttggcctt tttcatccag aaaacgccag tcgttaaaca gatgcgcgcc aaaaccagta
     2521 cgggtcactt tctgcaaaaa ctgtttcggg atgattgcat cggtatcgac attggcggca
     2581 tccagcggaa ccaccaggcc tgtgtgtttg ataaatttct ctgccatggt gtgctcctta
     2641 tttaatgttg cgaatgtcgg cgaaatggcc ggtcacagcc gcggcggcag ccattgccgg
     2701 gctgaccaga tgcgtgcgcc cgccgcgccc ctgacggcct tcaaagttac ggttgctggt
     2761 ggaggcacaa cgttcgcccg gattcagacg gtcgttgttc atcgccagac acattgagca
     2821 gccaggcaag cgccattcaa aaccggcttc aataaagatt ttatccagac cttccgcttc
     2881 cgcctgcgct tttaccggac cagagccggg aaccaccagc gcctgtacgc ctggcgcgac
     2941 tttccgccct ttggcgattt ccgccgccgc gcgtaaatct tcaatacgtg agttggtgca
     3001 ggaaccgata aacactttgt cgatagccac ttcggtcagc agaatacccg gtttcagccc
     3061 catataggcc aacgcttttt ctgccgacgc gcgttcaacc ggatcggcaa acgaagccgg
     3121 atcgggaata ttgtcgttca cggaaatcac ctggcctggg ttagttcccc aggtgacctg
     3181 cggtgaaatc tcttctgcct gcaaagtgac aacggtatcg aaagttgcgc cttcgtcggt
     3241 ttgcagggtt ttccagtagg caacggcgtc gtcgaaatct ttgcctttcg gcgcatgcag
     3301 acggcctttg acatagttaa aggtggtttc gtcaggtgca accagaccgg ctttcgcgcc
     3361 catttcgatt gccatattgc acagggtcat ccgaccttcc atgcttaaat cacggattgc
     3421 ttcgccgcaa aactccacca catgcccggt gccgcctgcg ctaccggttt taccgataat
     3481 tgccaacacg atatcttttg cggtaatgcc cggcgcggct ttgccctgga cttcaatttt
     3541 catggtcttc gcacggccct gtttcagggt ttgcgttgcc agtacgtgtt caacttcgga
     3601 agtgccgata ccaaaggcca gtgcgccaaa cgcgccgtgg gtggcggtat gcgagtcgcc
     3661 gcagacaatg gtcatccccg gcaaggtcac gccctgttcc ggccccatta cgtggacgat
     3721 cccttgatag gggtgattca ggtcatacag ttcgacgcca aattctttgc agtttttgat
     3781 cagctcctgc atctggatgc gcgccatttc accgcaggca ttaatgtctt tagtctgggt
     3841 agagacgttg tgatccatgg tggcgaaggt tttgcccggc tgacgtaccg ggcgaccgtg
     3901 ggcgcgcaga ccatcgaacg cctgcggtga ggtcacttca tgcaccaggt ggcggtcgat
     3961 atataacagc ggggtttcgt tttcggcttc gtacactacg tgagcgtcga ataatttttc
     4021 gtataacgtc ttagccatga ttacacccct tctgccacat agcgggcaat gatatcgccc
     4081 atttcatcgg tactaacggc ggcagcgcca cgggctaaat ccccggtgcg aatgccttct
     4141 tctaatgcgc ggttaatggc gcgttcaatg gcggaagccg catcatcggc atccaggctg
     4201 taacgcagca gcagtgccag cgacagaatt tgcgcaatcg ggttggcgat gtttttacct
     4261 gcgatatctg gtgccgagcc gcccgccggt tcatacagtc caaaaccttg ctcgttcagg
     4321 ctggcggaag gcaacatccc catcgagcca gtgatcattg cgcactcgtc agacagaatg
     4381 tcgccaaaca ggttggagca cagcagaacg tcaaactgtg atggatcttt aatcagctgc
     4441 atggtggcgt tgtcgatgta catatgcgcc agttcgatat ccgggtattc cgtggcgatc
     4501 tcgttgacga tttcacgcca taaaatagag gattgcagca cgttggcttt atcgatcgac
     4561 gtcactttat ggcgacgttt acgagcagat tcaaacgcga tgcgggcaat acgctcgatc
     4621 tcaaaacggt gatacacctc ggtatcaaac gctttttcat attgtccgct accttcgcgg
     4681 ccttttggct gaccgaaata gatgccgccg gtcagttcgc gcacgcacag gatgtcgaag
     4741 ccgtttgcgg caatgtctgc acgcagcgga cagaatgctt ccagcccctg atacagtttt
     4801 gccggacgca ggttgctgaa taatttgaag tgcttacgca aaggcaacag cgcgccgcgt
     4861 tctggttgct ggtccggtgg taaatgttcc catttcgggc cgcctaccga gccaaacagc
     4921 acggcatcgg cttgctcaca accttcaacc gtcgcaggcg gcagtggttg cccgtggtta
     4981 tcaatggctg cgccgcctac atcgtaatgg ctggtagtga tgcgcatcgc aaagcggttg
     5041 cgcacggcat ccagcacttt cagcgcctgg gtcatcactt ccggaccaat gccatccccc
     5101 ggcaatacgg caatatggta attcttcgac atcacacggt ttccttgttg ttttcgttgt
     5161 gttgagcttt gcgttgcaac tctttttcga cttctgtggc acgccagata ttgttaagta
     5221 cgtgcaccat ggctttggcg gaggactcga caatatcggt ggccaggccg acgccgtgga
     5281 agcggcgacc gttgtagttg gcgacaatat ccacctgacc cagcgcatct ttaccgtgac
     5341 ctttggcggt caggctgtat ttcaccagtt cgacgttata gtcagtgatg cggttaatcg
     5401 cctgatagac ggcatcgacc ggaccgttac cgttggcggc ttctgctttg acttcttcgc
     5461 cgcaggccag tttgacggcg gcggtggcga tatcgttaga gccagactgc acgctgaagt
     5521 aatccagacg gaaatgctcc ggctcttctt gctgcttacc gatgaaggcc agcgcctcca
     5581 gatcgtaatc aaacacctga ccttttttgt ccgccagctt gaggaaggcg tcgtacaaat
     5641 tgtctaaatt atattcactt tctttatacc ccatctcatc catgcgatgt ttcaccgccg
     5701 cacgccccga acgagaggtc agattcagct ggatttggtt cagaccaata gattctggtg
     5761 tcatgatttc gtagttttcg cggtttttca gcacgccatc ctggtggata ccggaggagt
     5821 gtgcgaatgc gccgctgcca acaatggctt tgtttgccgg gatcggcata ttacaaatct
     5881 ggctaactaa ctggctggtg cgccatatct cctggtgatt aatggcggtg tggacgttga
     5941 gaatatcctt acgaacttta atcgccatga tgacttcttc cagggaacag ttaccggcac
     6001 gctcgccgat cccgttcatt gcaccttcca cctggcgtgc accggcatgt accgccgcca
     6061 gtgagttgcc gaccgccagg cccaaatcgt cgtgggtatg tacggaaata atggctttgt
     6121 cgatgttagg cacgcgttca tacaggccgc taatgattcc ggcgaactca aacggcatgg
     6181 tgtagcccac ggtgtccgga atgttgatgg tggtggcacc ggcattaatc gccgcttcga
     6241 ccactcgcgc cagatcggca atgggtgtac gcccggcatc ttcgcaagaa aattcaacat
     6301 catcggtgta attacgggcg cgtttcacca tatagatagc gcgttcgatc acctcgtcca
     6361 gcgtgctgcg cagcttggtg gcgatgtgca ttggcgaagt ggcaataaag gtatgaatac
     6421 ggaaggcttc ggcgactttc agggattcgg ccgccacgtc gatatctttc tccacgcagc
     6481 gagctaacgc gcatacacgg ctgtttttaa cctggcgagc gatggtttgc actgattcaa
     6541 aatcacccgg cgaagagacg gggaacccga cttccatcac gtcaacaccc atacgctcaa
     6601 gggccagcgc aatttgcagt ttttctttca cactcaagct tgcctgtaac gcctgttcac
     6661 cgtcgcgcaa tgtggtatcg aaaataatga cttgctggct catggtttgg gtccttgtct
     6721 cttttagagc gcctcgcttc gggcataaaa aaacccgcgc aatggcgcgg gttttttgtt
     6781 tgactgcgtg ctggcttaat gctggatgcc gctcactcgt ctaccgcgca aagaagatgc
     6841 gtttagtagt agtagaccga taaagcgaac gatgtgagtc attaaatcag ctccagatga
     6901 atgcgatatg cttttagagt tactggatac aaaaacggat gtcaaccctg acgcaataaa
     6961 aacgtcccgc cagcatgagt tctgcatccg taagattagc taattgtgct gctgtggtta
     7021 aaacaagcga tattaatttc tgcttaacta ccgatgcttt tcatcggttg acatatttca
     7081 gaataaattt ttgcatctaa tccacgagga aaaaggggac aaaatgcacg cgttgcaaaa
     7141 cctatcctga tgatttgtat tgaattatat gttttgcgga ttttttgata ttgatttggt
     7201 gaat
//
LOCUS       DAZFBR010000182         7075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFBR010000182 DAZFBR010000000
VERSION     DAZFBR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.182"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(258..1394)
                     /locus_tag="ACHJZ2_004609"
     CDS             complement(258..1394)
                     /locus_tag="ACHJZ2_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4570580.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1397..1759)
                     /locus_tag="ACHJZ2_004610"
     CDS             complement(1397..1759)
                     /locus_tag="ACHJZ2_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO4570581.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERVWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2296..4209
                     /gene="mtlA"
                     /locus_tag="ACHJZ2_004611"
     CDS             2296..4209
                     /gene="mtlA"
                     /locus_tag="ACHJZ2_004611"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO4570582.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDPSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4304..5452
                     /gene="mtlD"
                     /locus_tag="ACHJZ2_004612"
     CDS             4304..5452
                     /gene="mtlD"
                     /locus_tag="ACHJZ2_004612"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO4570583.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5452..6039
                     /gene="mtlR"
                     /locus_tag="ACHJZ2_004613"
     CDS             5452..6039
                     /gene="mtlR"
                     /locus_tag="ACHJZ2_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO4570584.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6051..6260)
                     /gene="yibT"
                     /locus_tag="ACHJZ2_004614"
     CDS             complement(6051..6260)
                     /gene="yibT"
                     /locus_tag="ACHJZ2_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO4570585.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6545..6907
                     /locus_tag="ACHJZ2_004615"
     CDS             6545..6907
                     /locus_tag="ACHJZ2_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO4570586.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1801 a         1779 c         1797 g         1698 t
ORIGIN      
        1 agatggaaaa gcactcctgc tcttttttga ttagagccgt cgcaggagga acaactcata
       61 tcatcattgc gatctcgacg atgtatagtc tcatcctgcg gcagaacaag acggataaaa
      121 aatccaatga gagcacactc atccagaact tattaacata atgaatatta aagaaaaatc
      181 atcgcagcta atgaagtaat atgcaaaaat aattagtcct tacaaggagc aaagttccat
      241 aatctccccc ctccccctca atgatccaaa taaagataat gcatccagct ggtcattctt
      301 agcagcactt tccgcatcac cgaaacatgg ctgaaatggt cggagtaaac cgccacctgg
      361 gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag ggtccagttc aatggttccc
      421 agcacaccgt ccgtgccggg cacgaccgtt aatgattgca ataccccctg cgcctgataa
      481 gaaccgcctg gcacgacagg taaaatacta gtcagtttgc cgtgaaacac ctgcccaggt
      541 agcgcgttaa acaccacttc cgcatcatcg ccgggtttca gacgtaacag cgagttttgc
      601 cgaaattggg cgacaatttg ccgtttttgc tcggggatga agaccatcac cggacgcagc
      661 ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct gagtaacgta gccattgctc
      721 ggcgcgcgaa tgacagtctg ctcaaggtta tattttgctt cagtaagttg cgctcttaag
      781 ctcacaatct gcgattgctc gccgttaacc atactgtcga gctggctctg gatctgcgcc
      841 tgctccgcca ccgagccttt caccagcgca tcctgcgcga ggaaattttg ccgcgcatcg
      901 tcgatgtcac gttccgagaa cggattcacc gccgcctggc tgcctttcaa gtaacgttga
      961 taatttttaa acagacggtc gcgctccgct gaaacctggg tggtgttggc ctgcgcttca
     1021 gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg tcatcaggtc agcctgaagt
     1081 ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa aaagcacctc gcccttttga
     1141 ataagctgat tattcttgtc agtgacttca gtaacaattc ccgtcacctg tggcgtgata
     1201 gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag ggtggttgta gttcatcaat
     1261 aaaatcaaac cactcaccag aaacacgcct cccagcgccg ccgtcgccag cgtccactga
     1321 tttaccggaa tgcggaagat tttaaagacc gcccacgcca gcgccacgta agttaaaaca
     1381 atcaatagat ccataattag atctccggga acgtggaata gtcagtcttt ttctcggcgg
     1441 cgagttgatg ctccagccgg gaaattcggt cagaaagtga ggcgatttcc gggtcagtcg
     1501 ctttctcctg cgacgcaaca tgtgactgca taccccagac acgctccggt tgatagagcg
     1561 tcgcccagat ccacagaaac ggccagataa catgcagagt aaacaggctc acccagcccg
     1621 ccgtatgaat agcgtcggca tgggggtgat tgcgcttttt ggcaatcaga taagggatgt
     1681 catgtatcgc gatgattcca taaaaaatca ccagaaagac gaagatcagc actcccaacg
     1741 cgaaatagtt tagaaacata tctgcctcgg attcacgttt atcagtgttg tttttgggct
     1801 ggcagccaga agggagtcag gctgatattt tgacaataat ccgggttcgc gattctcgcc
     1861 ataacaccaa agaataattt ttagaggtga tgagttgctt agttacataa cgattgtatg
     1921 acgaaggcat aacatgctgt agatcacatc aggtgaacgc cgtaagaaaa tatcttgtga
     1981 ttcagatcac aaagattcaa caaaccatca aaacaaaaat gtgacactac tcacatttaa
     2041 atgccatttt tagcgaaaat cgccgccttg ttgctttttt acacaagcgt tttgtgatga
     2101 acgtcacgtc aattacctct ctacccccta tatttatgtg attgatatca cacaaaaggc
     2161 catcgactgg acagttaacc gattcagtgc cagatttcgc agtatctaca aggtccggct
     2221 acctctgccg ccacattaac aaaaaacctc gggcttccag cctgcgcgac agcaaacata
     2281 agaaggggtg tttttatgtc atccgatatt aagatcaaag tgcaaagctt tggtcgtttc
     2341 ctcagcaaca tggtgatgcc aaatatcggc gcgtttatcg cgtggggtat catcaccgcg
     2401 ttatttattc caacagggtg gttaccgaac gagacgctgg cgaagctggt cgggccgatg
     2461 atcacttatc tcctgccgct gctgatcggt tataccggtg gtaagctggt aggcggcgaa
     2521 cgtggcggcg tagtcggtgc catcaccacc atgggcgtta tcgtcggcgc agacatgccg
     2581 atgttcctcg gttctatgat tgcaggtccg ctgggcggct ggtgcattaa gcacttcgac
     2641 cgctgggtag acggtaagat caaatccggt tttgagatgc tggtgaataa cttctccgca
     2701 ggcatcatcg ggatgatcct cgctattctg gcattcctcg gcattggccc gattgttgaa
     2761 accctgtcca aaatgctggc tgcgggcgtt aacttcatgg ttgtccatga catgctgccg
     2821 ctggcgtcta tctttgttga accggcgaaa atcctgttcc tcaacaacgc cattaaccac
     2881 ggtatcttct cgccgctggg tattcagcag tcccatgaac tgggtaaatc aatcttcttc
     2941 ctgattgaag ctaacccagg tccaggtatg ggcgtgctgc tggcgtacat gttctttggt
     3001 cgtggtagcg ctaaacagtc tgcgggcggt gcggcaatca tccacttcct gggtggtatc
     3061 cacgaaatct acttcccgta tgtgctgatg aatccgcgtc tgatcctcgc agtcatcctc
     3121 ggcggtatga ctggcgtgtt cacgctgact atcctgggcg gtggtctggt ttctccggca
     3181 tctccgggtt ctatccttgc tgtactggcg atgacaccaa aaggtgctta tttcgctaac
     3241 atcgcgggtg tgtgtgcggc gatggctgtc tccttcgttg tctctgctat tttgctgaaa
     3301 accagcaaag tgaaagaaga agatgatatt gaagcagcaa ctcgtcgtat gcaggacatg
     3361 aaagctgagt ctaaaggtgc atctccgctg tctgcgggcg atgtgactaa cgacctgagc
     3421 cacgtacgta aaatcatcgt tgcctgtgac gccggtatgg gttccagtgc gatgggcgca
     3481 ggcgtgctgc gtaagaaaat tcaggatgca ggtctgtcac agatttcggt cactaacagc
     3541 gcgatcaaca acctgccgcc agatgtggac ctcgtcatca ctcaccgtga cctgaccgaa
     3601 cgcgctatgc gccaggttcc tcaggcacag catatttcgc taaccaactt cctcgacagc
     3661 ggcctgtaca ccagcctgac tgaacgtctg gttgctgccc agcgacatac tgaaaacgaa
     3721 gtgaaagtga aagacagtct gaaagacagc tttgacgatc ccagtgctaa cctgttcaaa
     3781 ctgggcgcgg agaacatctt cctcggtcgc aaagcagcaa ccaaagaaga agcgattcgt
     3841 tttgctggcg agcagctggt gaaaggcggt tacgttgagc cggagtacgt tcaggcgatg
     3901 ctggatcgtg aaaaactgac cccgacttat ctgggtgagt ctatcgcggt gccacacggt
     3961 acggttgaag cgaaagatcg cgtactgaaa acgggcgtgg tgttctgcca gtacccggaa
     4021 ggcgtgcgct tcggtgaaga agaagatgac attgctcgtc tggtgattgg tattgctgcc
     4081 cgtaacaacg agcacattca ggttatcacc agcctgacca acgcactgga tgatgagtcc
     4141 gtcatcgagc gtctggcaca caccaccagc gtggatgaag tgctggaact gctggcaggt
     4201 cgtaagtaat ccaatcctac cctctcctca cggagagggt ttgggtgagg gaaaagcctc
     4261 accccagccc tctcgggtaa aaacattgat gaaggttaat actatgaaag cattacattt
     4321 tggcgcaggt aatatcggtc gtggctttat cggtaaactg ctggcagacg cgggtatcca
     4381 actgacgttt gccgatgtca atcaggtggt acttgatgcc ctgaatgccc gtcatagcta
     4441 tcaggttcat gtggtcggtg aaaccgagca ggtagatacc gtttccggcg tcgatgctgt
     4501 cagcagcatt ggtgatgatg tcgttgatct gattgctcag gtcgatttag tcactaccgc
     4561 cgttggcccg gttgtgctgg aacgtattgc tccggctatc gccaaagggc tggtgaaacg
     4621 taaagaacaa ggtaatgaat ccccgctgaa catcatcgcc tgtgaaaaca tggtacgcgg
     4681 taccacgcag ctgaaaggtc atgtgatgaa cgccctacca gaagacgcca aagcgtgggt
     4741 agaagaacac gttggctttg tcgattccgc cgttgaccgc atcgtaccgc cttcggcttc
     4801 ggcaactaac gatccgctgg aagtgacggt agaaactttc agcgaatgga ttgtcgataa
     4861 aacgcagttc aaaggcgcac tgccgaacat cccaggcatg gagttaaccg acaacctgat
     4921 ggcatttgtc gaacgtaaac tcttcaccct gaacacgggt catgctataa ccgcgtacct
     4981 cggaaaactg gccggtcatc agaccattcg tgacgctatt ctcgacgaga aaatccgcgc
     5041 ggtggtaaaa ggtgcgatgg aagaaagtgg tgcggtactg atcaagcgct acggctttga
     5101 cgcagacaaa catgcggcgt acatccagaa aatcctcggt cgttttgaga acccgtatct
     5161 gaaagatgat gtagagcgcg taggccgtca gccgctgcgt aaactgagtg ctggcgaccg
     5221 tctgatcaag ccattgctcg gtacgctgga atacagcctt ccgcacaaga atctgattca
     5281 ggggattgct ggtgcaatgc acttccgcag tgaagacgat ccgcaggctc aggaactggc
     5341 agcactgatc gctgacaaag gtccgcaggc ggcgctggca cagatttccg atcttgatgc
     5401 caacagcgag gttgtatccg aggcggtaac cgcttataaa gcaatgcaat aatggtggac
     5461 caggcgcagg acaccctgcg cccgaataac agattgtcag atatgcaggc aacaatggaa
     5521 caaacccagg cctttgaaaa ccgtgtgctt gagcgtctga atgctggcaa aaccgtgcga
     5581 agctttctga tcaccgccgt cgagcttttg accgaggcgg taaatcttct ggtgcttcag
     5641 gtattccgca aagacgatta cgcggtgaag tatgctgtag aaccgttact cgacggcgac
     5701 ggtccgctgg gcgatctttc tgtgcgttta aaactcattt acgggttggg cgtcattaac
     5761 cgccaggaat acgaagatgc ggagctgctg atggcattgc gtgaagaact aaatcacgac
     5821 ggcaacgagt acgcctttac cgacgacgag atccttggac cctttgggga actgcactgc
     5881 gtggcggcgt taccaccgcc gccacagttt gaaccagcag actccagttt gtatgcaatg
     5941 caaattcagc gctatcaaca ggctgtgcga tcaacaatgg tcctttcact gactgagctg
     6001 atttccagaa tcagcttaaa aaaagccttt caaaagtaag caacgtctgc ttactgcccc
     6061 tctacctgct tcggccgata aagccgacga taatactcca gacgttgtag atataacggc
     6121 acgctttcat cgggtattcc ggacggaatc gcgttacggg gaggaagttt tttcagatac
     6181 tcccggaacg cctggcttga tgccatgaaa tctacggctt tatcgataag aagcggaacg
     6241 ttttcaccta atttgcccat gatgatatct ccgtattacc ccgcgccggg aatgcgcggc
     6301 cgccaatttt agtttaggtt cccggtaaag atgacattaa ggaaaacgtg ctgaatcctc
     6361 aataatcatc cgctgctgtt tattatcttt tttctataga tttcttaatt aatcaataaa
     6421 ttgcgtagtt tttaagcatg ttaatgatta cgttacagaa ctattgcaat gtcatcagtt
     6481 gtgaacaaag cacctggtcg cgcatactag gggctataaa tttatcttta tcagaagcca
     6541 tcacatgaaa gaagtcgaaa aaaacgaaat caaacgtctc agcgatcgtc tggacgccat
     6601 ccgccaccag caggccgatc tgtcgctggt tgaagccgca gacaaatatg ccgagctgga
     6661 aaaagagaaa gccacgctgg aagcagaaat tgctcgcctg cgtgaagttc atagccaaaa
     6721 actgagtaaa gaagcacaga agctgatgaa gatgccgttt cagcgcgcaa ttaccaaaaa
     6781 agagcaggct gatatgggca agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc
     6841 aatgaccgcg ctgggccgtg aaatgggcct gcaggagatg actggattct ctaagactgc
     6901 gttttaagcg cctcagacga ctggttcgat catatcaacc cgcatctggc ttgccttagc
     6961 tggtcaatgc ggggactctc ctgtctactc gcctaattac caaacaattc actcccttta
     7021 cgtcgattta ccgctggttg atattctgaa aatgcatttt taccgaaata gccac
//
LOCUS       DAZFBR010000183         7062 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFBR010000183 DAZFBR010000000
VERSION     DAZFBR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.183"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(35..2818)
                     /locus_tag="ACHJZ2_004616"
     CDS             complement(35..2818)
                     /locus_tag="ACHJZ2_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO4570587.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2875..5247)
                     /locus_tag="ACHJZ2_004617"
     CDS             complement(2875..5247)
                     /locus_tag="ACHJZ2_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO4570588.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5285..6970)
                     /locus_tag="ACHJZ2_004618"
     CDS             complement(5285..6970)
                     /locus_tag="ACHJZ2_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO4570589.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGMLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1827 a         1612 c         1588 g         2035 t
ORIGIN      
        1 aatccccgcc aacgatgtgt tgacggggct gttattattt tggcaataat actccggtat
       61 aagtatttac cggatgagaa agatattgtt taacggcagt gttaacattc tctaccgtca
      121 tttgtttcaa caattgctcc tgctcagtcc atgctgcagg atcgtcatat tgaataagac
      181 tatttacaat agtgttcgct aattgttgaa cgctacgctg ttggatatcg aggctgcgct
      241 gaacgttttg ctggtattcg ttcagttctt gctcactgat ccctttagtc aaacgcttaa
      301 ccatcacttc attcgctaac gttaacagtt catcatgtcg ttctggttga caagtaaaag
      361 ccagcaaatg actgatatct ttagcctgag gatcaaccga gaggcgagaa gaaacgctgt
      421 atgccccaga tgcctgttca cgaatattaa cgcgtagatc ttttgccagt gcgacgttaa
      481 aagcatcgag cgccatacgc gtcgccagat taacaggtgt ccgggaatca taacgcttcc
      541 actgtgaaac ctgtgccaca ggttcatttt gttcctttac agtaaccgat gcgttgtccg
      601 tcgcgcgagt taatggttta cctgcggcta atggcgaatc agagtgtttg attgatccta
      661 agtaacgcgt aattaacgcc acgagtttgt cttctgcgac attaccgaca atgacaaacg
      721 tgatatccgc tggagatgaa aacaactggc gatcggcagc cagcgcatct gcggcagtaa
      781 actgtgcaat ctgattttct tgcagtaatt tcgtgcggtc atcagcatag cgcgtctcat
      841 acatctgctg ggcgaatttc tccgctggac gctggtcgag cgttttcaat gcctgaattt
      901 gagcattttg tagcgatgcc caaatattgt cgttaatcgt gctgtgggtg attcgctggt
      961 taatcaactg aaaaccaggt tcagggttat tagtccgcgc gctaacggag agcaacgtat
     1021 tcatgccact gactttactg ctcatggtta ccgagttttc cgcactccag cgtttcaggc
     1081 tggaagagga gagttcgcca acgccgcttc cgctaactgc tttatttgcc agcgcgataa
     1141 gtgatttttg ctgcgcaggg aaacttaaat cgcctttatt cgatacggca ataatttgta
     1201 gcttttgctc ttcacccgcg gattttgcca gaataaccct tgcaccattg gaaagtgtta
     1261 atgatgtcag attctcagcc agcgtttctt tgctgctaat ttccgcctgt gggtcagcgt
     1321 ctactgttaa cgataaattt ctgcctggga agatgtaagc cgccagcttt ttgttggcgt
     1381 actccttttc cagcgccagg atagccgcag gagacaatgc ttttttggcg gcaacctcat
     1441 tgtttaccat ttgctcccaa aatgcgtcct ggttctttct taactgctgc catttttccg
     1501 ccagactttg cacggtaatt tgctgccaca gacgtttcga aagttgatat gtctcttccg
     1561 gcgacaagaa cggcgtatta tttaatgagc tggatgccag gcgactggtc agcatacgta
     1621 aatcacgttc agcttgctga tcaaccgcat ttttcagcca ggtgaggcgg gtagatttga
     1681 catcatcgag ttcttcagca gaaaaaccat gctgatcaat ggttgccaac tcttccatta
     1741 atgcattcgc agcatcctgc atattatcgt ctcgtgcatt tacacggaaa aacagcgact
     1801 gataatcggg tgcaatttta acgctgcgcg cagtgccgcc agaaatagtc ttcaactcgc
     1861 ccgactgtat acgttcctgc agacgttgat tgaacagctg aactaacatg ctccattcag
     1921 cttgttcgat aaagctttgc tcatcgttca cttgtaccat tggcaggcga taatagagtg
     1981 cgatgccgtt cacccggttt tctttatcat taatgatatt aaagcgcagg tggttttcgg
     2041 cttttgtcgg ccagacgcga ttttcagctg ctttgttagc cggaagctta cttaaattat
     2101 cctttatcag cgccagcgct tctttactgt cgatatcgcc aaccacgata aaggtcatat
     2161 tatttggttg ataccagcgt tgataaaatt ggagcaattg tgccggtgtg accgtggcga
     2221 cagtatccat caggccgata ggttcacggt ctaaattacg ggtatttgcc agcaggaaag
     2281 ggcggcgcgc ctgagaggtg cgccatttcg catcctgatg ggcacgccat tcctcagtaa
     2341 ttacgccgcg ttcagcgtct acttcgagtt tttcaaaggt tgcggcatta ctccattcac
     2401 tgaagattgc catcacttgt tgcagatttt gtttctgcgt agttggcaaa ctcacctgat
     2461 acaccgtttc gtcatagctg gtataggcat taacatcgcg accaaaacgc aggcccattg
     2521 actcaaatgt ttcgatgact ttattacccg gccatgtttt tgtgccgtta aacatcatat
     2581 gttctacaaa atgagccacg ccgcgctcat tgtcttcttc ctgcaatgaa ccggtatgaa
     2641 tttgcagcca taaatttacc tgatcctttg gatgagcatg cggataaatc atatatcgca
     2701 agccattgtc cagttgcccg gtaattaact tttcatcctg cggtaaggcg gcggcaatca
     2761 gccgcccggg gagcaacaga gttgccacta acgtcagtaa gaaacagagg tttctcataa
     2821 ttatctccat gcgaaaaccg ggcgaattta cccggttaag taaaatccga actattaaaa
     2881 tttcatgctg acatcaagcc agaaagtacg gccactggcg taggtcgcca caccggtatt
     2941 cgttgtatca accgcggttt tgctatccag tacattgaga atatcggcac tgatcgtcag
     3001 gttttgttgt ttcagaaatt gtggcgtcca ggacaagcgg gtgtcccagg tcaggctgct
     3061 atccagtttt tcgtcaacat actgcttgta atctgaatat tcgctgatgt attgcgcatt
     3121 tgtcttaccc aggataatgc gagctttacg cgcttcttgc caggccagcg tattcgccca
     3181 caccaacccg ctcggttgat gcgtgaaatc catgtttaag gagatcttta atgggttatt
     3241 aaaatctgcc actggaacgc tatcgtaaga gacaagatta ccgttataaa ccacctggtt
     3301 atcaccggta ttgctctcct cataaccatt gtttaacgac aagttgccct tgctcttgat
     3361 atagctaaag acaatttgcg ggttaatatc tacctggctg atatgcaggg gttcggccag
     3421 ttcaaaactg aggttgaacg aatgcgtttt ggttttgccg tcgttgttat attcagtaat
     3481 ggtggtttta gtcgcgctgt cggtacgact gcttttgctg atttgatcat gcgcttcacg
     3541 gtaaacatag tttgcgcgcg caataacgtt cttaccgatt ttttgctgca atcccattgc
     3601 cagttcatcg ttataaggcg ttttcaaatc ctgataacgc gtcagggttt tattacctga
     3661 taccgattcc gtccagctat tgcggatatc acgtaatccc atatcaagaa tattcccgcc
     3721 atagtaacgg ttataacctg ctgtaatcat tgaggtttga tcagcaaaaa tatcccattc
     3781 cgtcataaag cgcggggaga tattgtggtt tgacagatag ttgtcatagt cataccgcac
     3841 gccgggcatt aatgacacat tacgccagct aatgcgatcc gccatataca gtgtgtagtt
     3901 gtcaattccc aggctgcctt tacctttatg gtaaatggta tggttagttt tctttccggc
     3961 agcattaatc acataggatt cagactggtt atggcgttca gtccacgcat cggaatagat
     4021 gtattccgcg ccgaagtagg gttgatgcga aacattaccc acggcgaatt tttgccagtc
     4081 caggcgtgtt ttgaaggtgt aattatctac agcctgggaa atgtgtccta atccgccacg
     4141 ggtgcaacgc cctgtaatat caccatatgt acatgaaagt tcggtgtacc agatgtcatg
     4201 atcgtgacgg gtataatcac taatatgatc ccaaccaacg gtggtacgta gtttggccca
     4261 tgcgagctgc gtatccatat cccatgccat accataggat ttattaccca tttcgcgatc
     4321 agactgcggg aaggtgctgg tattataatc acggctggag ccggtatatt ttaaggttaa
     4381 atcgtgggta aagcggtcgc tggcaaacca ggtaaatttg ctcaatgcag tatcgataac
     4441 gtttttatac tgtgcccgac cggcgacaat gccgtcattc gaaacataat ccgcgcgggt
     4501 gatatcagac tggcggcgcg ataaaccggc ggtaacgcca aagttatcag cgagttcctg
     4561 attaaacgac aaggtataaa agttcttttt aaaatctggg gagtaataag tacttcctga
     4621 agagccttga ttaaatgcgc tcttgttatt ctcattgata tgcgatgtta accagtccga
     4681 acgcgtagtg cgataaccca gtttcacgct gctatcatca gcgttgaagc gtttgaactt
     4741 tgcatcaatt accccgccat tgaagcgacc gaattcaacc ggcacaaaac tgtcataaag
     4801 cgtcacattg tccagtaagc tgacatcaag ataatacccc tgtgacatcc cgctaatatt
     4861 ggttgcacta ctggcatcgg actcattcgc tgggttcagg ttattagtgg cactaatacc
     4921 gtcaatcaaa taggcattct gatagggcga cgcaccgtga atagagattt tctccgggcg
     4981 aatatctccc tggttcaacg aggtactttg cgttgaatcc atgcgtaccg cagggttggt
     5041 tctcagcaga tcgctgatat tgccattccc ggtcggtaaa cgctcgatgc tttcactggt
     5101 gtaatgcgtg ttgccgttta cgggtacagg gacaggagca tagaccgtca tctcttcaaa
     5161 aaaatgcatg tacatgtttt tgtcatcgac ggcctgcgcc acatcagcgc cacataaaat
     5221 gacgccagga ataagaactc gcttcattta tttatatctc cgtacagcag ggcttttatt
     5281 gtttttataa aaccgcgcta atgtcacaaa tatcatcggc caggttccag acaccgggtt
     5341 gatgtgtaac cataatgacg ccgcttgtgg gtagtttttc acgcactaga cgcagtaagc
     5401 ggatagcctc ttgttcctca agatgagagg tagtttcgtc aagaaatatc catttcggac
     5461 gtcgtaaaat taatcgtgcc agggcgatac gttgtttttc gccgctggaa agaatatctc
     5521 cccagcgatc gtggtcatga atacgcgcag ccaatttccc aagaccaacc tgatgcagta
     5581 cttcgctcaa tgatttatcg tctacgggca ggggaagtgc tttacaaata atctctttca
     5641 gtaagccggt tttgattaac ggtgtttgtg acacatacca actgtcggca ggagaagaaa
     5701 tatcaccttt aaaccacggc cagcagtggg ataatgtttt aagcagtgtg gtttttcccg
     5761 caccagagta acctttcagt aatagccatt tgcctggcga aacatgaaag ttcaggttct
     5821 ctaatatgat cttattatca ggagtacgaa tactcgcgtc agccacttgt accgcatgtt
     5881 ggcaattttt aggcttattc gtagggcgct gttcggtgag ttgatggaac tcatacaagc
     5941 gatcgataac cgcagccagt tcagcaagtt catcatattt ataaataaac cagcttagat
     6001 tgttcgatac cagcataaat gcctggcgcg atttcatcag tccgcccaga ttgatctgcc
     6061 cgctaataaa ctgcggcaat aacagaaagt agggaagaac gctaagcgag cgcgaataaa
     6121 tattctgcca gtaatcaagc caccgctgac gattcattaa acgatgccag ttctctttaa
     6181 tggtatgaaa attgtcgctc aactcctggc gttgtagaga ctcagcattg cttagcgcaa
     6241 tcagttctgc ctgcttatta tgctgcacaa gattagttcg aaatgtcgct tcgctacgtt
     6301 gtttttccac attaagcgga cgaatacgtt tacccacctt atgggtaaat aaagttccac
     6361 cgatcacaat gagcacaacg gtatagacca tatacccctg gatattccat tctgttccac
     6421 caacagtgaa tgagagcata cccgcgcttt gccaaagaat aacggtaaag gtgatcagca
     6481 tgctaagtga ctggatgaag ccaaatgaaa gactgagcgt tttgctgatc agtaagagaa
     6541 tgtcttcagc gatacgttgg tcagggttat ctgtattttt atgctcgccg tagatctgcg
     6601 tgaagtagta gtttttatct gcaaaccagc gattaaggta gtaatctgtt aaccattcgc
     6661 gccagcgaat agtcagtagt ttaattaacc atgttttgtt tacggatatt aatacaaaga
     6721 tccccagcag ggcagggaac caaaggacaa gctgccaaag cttatcggtt tctttctggc
     6781 ttagcgcatt gaaaaaatca ttattccagt cgtttaacca gacctgaatt ttaaccacgc
     6841 caaggatcat ggcgaggata ataatgatta acaacactga agttttatta ttcttacgca
     6901 gccaaaaggg cttcagcaga cagagatact ttgctatcaa catacaaaac gtaatgggaa
     6961 tggttatcat tagcgaaaat tgattgtgcc atccaatgat tatctatggc aatgatttac
     7021 tgatgaaatt tatttaagta tcatttaaca atatgaaata ta
//
LOCUS       DAZFBR010000184         7004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFBR010000184 DAZFBR010000000
VERSION     DAZFBR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.184"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(33..440)
                     /locus_tag="ACHJZ2_004619"
                     /pseudo
     CDS             complement(33..440)
                     /locus_tag="ACHJZ2_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(674..1564)
                     /locus_tag="ACHJZ2_004620"
     CDS             complement(674..1564)
                     /locus_tag="ACHJZ2_004620"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO4570590.1"
                     /translation="MYAPDSALPVDLIEQQCWSRATDTYGVKHQRKERPCEEKESYRW
                     QQASERMAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRRAGSPPGPD
                     AHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYE
                     QRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISEETQNDSCE
                     KILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGW
                     FRHQDMAEGYLVMKSLDQEI"
     gene            1574..1807
                     /locus_tag="ACHJZ2_004621"
     CDS             1574..1807
                     /locus_tag="ACHJZ2_004621"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570591.1"
                     /translation="MSQQPVRLRVSGGIPQVIKCRTVTEVNGGGIFQREEQRMCLTCP
                     CSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            complement(1739..1942)
                     /locus_tag="ACHJZ2_004622"
     CDS             complement(1739..1942)
                     /locus_tag="ACHJZ2_004622"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFO4570592.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            2117..2293
                     /locus_tag="ACHJZ2_004623"
     CDS             2117..2293
                     /locus_tag="ACHJZ2_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570593.1"
                     /translation="MVNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIAT
                     EMCGISPGTVTCKR"
     gene            2854..3414
                     /locus_tag="ACHJZ2_004624"
     CDS             2854..3414
                     /locus_tag="ACHJZ2_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4570594.1"
                     /translation="MISVMILYVMSGSRLADNHTLSAGYAQSKVQDFKNIKGVNLQYR
                     YEWDSPVSVVGSFSYMKGDWADSHRDEADDFYRHQADIKYYSFLAGPAYRLNDYISFY
                     GLVGISHTKAKGDYEWRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMSVNV
                     GYEGTKADIYGKHSVNGFTVGVGYRF"
     gene            3550..4341
                     /locus_tag="ACHJZ2_004625"
                     /pseudo
     CDS             3550..4341
                     /locus_tag="ACHJZ2_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(4346..5716)
                     /locus_tag="ACHJZ2_004626"
                     /pseudo
     CDS             complement(4346..5716)
                     /locus_tag="ACHJZ2_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5736..6083)
                     /gene="tnpB"
                     /locus_tag="ACHJZ2_004627"
     CDS             complement(5736..6083)
                     /gene="tnpB"
                     /locus_tag="ACHJZ2_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4570595.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(6080..6754)
                     /gene="tnpA"
                     /locus_tag="ACHJZ2_004628"
     CDS             complement(6080..6754)
                     /gene="tnpA"
                     /locus_tag="ACHJZ2_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO4570596.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT         1658 a         1644 c         1825 g         1877 t
ORIGIN      
        1 tcagccagtt tcagattgaa catattcagc tattcaaaga gcaattcttc attaaactca
       61 ataaattgtt ccctcagtgt tcggttaaaa tgctcactta tggcatttat tttcggcgta
      121 tagagataag tccatacgaa cccgacggtg gcttcctgta gcgttttgtc gaattttccc
      181 gagaactctt tgcccatcat cagtgatgat ctggttgata ccaacaagga acaactgggc
      241 tacacagcag aagaaatgcc tgatgatatc acttttaagc gatagtacgg ccagagagta
      301 gttgctgtgt tcgtcgagca tggtaatgaa ataacggtac agctcgccat tctgagctca
      361 atagtatcca tgccactgag cttacaggtc tttatcgggc gatagtgctt tattcttcgg
      421 ggctttgtgg atgatttttc gaaatgttca tgatagttag gtaaagctac cgcatatcat
      481 ttgcaaaatg acagatagat atacttcaat ttgaatctta acaattagta tgggaatgct
      541 gtcaatcctc tgtatgcgtc caccacatat tgaaattttt gttctctaat atattccacg
      601 ggcgtcaaga gtgtgaggcc tgtgctgtta tacagcacca gacgttgtcc cccatctttg
      661 acctgtctct tgatcagatc tcctgatcaa gagacttcat caccaggtaa ccctcagcca
      721 tatcctgatg tctgaaccag ccatcccaca taactaccca accggggctg cttgtgcgtt
      781 tgctgtcatg ccatcgcccc agtttagcca gtttcaaaca ggcccatttc agggtcggtg
      841 tttgcgccgg aagctgcttt ccttccagct taacccacag tagtttccac tctgtcggcg
      901 tcagtatttt ctcacagctg tcattttgtg tttcttcact gatacctccc tgccgcaggc
      961 ccagcacccg caccgcgata accaccatgc gctcaaggtt atcccgggtc tgcattcgca
     1021 gcgattccac acatgtacca ccacttttcc acgctttgtg gtattcctct atcagccagc
     1081 gttgctcgta atggctgacg atacgtcgcg catcggcggc actcgccact ttttctgatg
     1141 tcagcggata ccagcaggct ccgtcctctc ctgctccccg caacagacat acgtgagcga
     1201 gagcgcctgg ccgctgttgt cgggattttt tatgctgact tcgctgtaac tgatgagcat
     1261 ccgggcctgg cgggctgccc gcccgccttt ttgcatcaca ttcagcgtgt ggcttcccgc
     1321 ggctgacttt ataatgcagg taatgccaga tatccgcttc ccggtcacag acagtgatta
     1381 cctgtttctg tatctccctc agccgttcgg ccatgcgctc cgaagcctgc tgccagcggt
     1441 aactttcttt ttcttcacag ggacgttctt ttcgctggtg cttaacaccg taggtgtccg
     1501 tggcacgact ccagcactgc tgttcgataa gatcgactgg cagggcgctg tcgggggcgt
     1561 acatcaggac agagtgagcc agcagcccgt gcgtcttcgg gttagtggtg gtattcccca
     1621 ggtcatcaaa tgccgtactg tgaccgaagt taatggtggt ggtatcttcc agcgcgagga
     1681 gcagcggatg tgcctcacat gcccttgcag tggcagtaaa tccggcttcg gcaatggctt
     1741 acggggacac cgacgggtta cgtatcaggc ggtacgcacc ttcaacctga gcagtgaagt
     1801 gggatgattt cacaatagaa agacctgcat gctgagcgag agaagaggtc agtgacacaa
     1861 ggcgtcgtgt acgacgcgga tcaccgagac gggcatgtcc agactgctcg ttagccagtg
     1921 aataacaatc agaaagtacc ataacagacc cgaagaaaat gaaatataag agaagatcaa
     1981 cgggtgaaga aaaagttcaa aaaatcgctg ccggggagga aggaaactac cggatggaaa
     2041 gagtccccct aaagcagact gacagacatc acaaatcccc gggtggggat ttgtgtataa
     2101 gagacaggtt acaggtgtgg tgaataagac tgcaaatgtg gcagctcatg ccgtggtgaa
     2161 tgctgcactg gcggtggtac agggaaataa cgcccgtgcc agtgccgcag gtgctgccac
     2221 tggagacgtg gtggggatga tagccacaga gatgtgcgga atatcccctg gcactgttac
     2281 atgtaaaaga taaataacga agctacatcc ttctctttat gaacggcttt atattagttt
     2341 tgctgacaag attgagctaa atccggccaa tgaagaagag caggttattc tttaaaggtt
     2401 tgataacatg taacacattt tgaatattca gtcagtaagc cttaaaaatt tacctggtta
     2461 tgggctgact gatataacaa agtaaatatt cgatggggaa tgccggatgc aatgaacaat
     2521 gcattaacgt atcgaaataa tgatgcagga gcaaactcag tctggtgagt atacattatg
     2581 ccattgatgc agagtttaaa ggaacagggc tggttcgtta tggaacaaca tttgtgcctg
     2641 aggaaagggg ggggagtatc tgaggcaaca gaagtttgtg gggagttcgg tttattacca
     2701 tgcttggttt tttgtcagtg tgttcaaatc gtcaggtgat atatttatag ttttttgatg
     2761 atgatttcaa ataatgtttt tgagatgtgt ggtccttttt gattttcaga aatcatttat
     2821 taatgaagga gggttaaatg aaaaaaactc gttataattt cagtaatgat attatatgtt
     2881 atgagtggtt caagactggc tgataatcat acgctatcgg ctggttatgc tcagagtaag
     2941 gtacaggact ttaagaatat taaaggagtg aatctgcaat atcgatatga gtgggattct
     3001 ccggtcagtg tcgtcgggag tttttcgtat atgaaaggtg attgggcgga cagtcatcgc
     3061 gatgaagctg acgattttta cagacatcag gctgatatta aatattattc atttctcgca
     3121 ggcccagctt atcgtctgaa tgactatatc agtttttatg gtttggtggg gatatctcat
     3181 actaaagcga agggagatta tgagtggaga aacagtgtgg gtgctgatga gtctgatgga
     3241 tatttaagtg agtctgtatc aaaaaaatcc actgattttg cttatgcagc tggtgttatt
     3301 attaatccgt ggggcaatat gtctgtaaat gttgggtatg agggaactaa agcagatata
     3361 tatggaaaac attcggtcaa tggatttact gttggagttg gttatcgttt ttaatcatta
     3421 gacgggactg gataattgca cttgcgtagt gaagtttact aaatgtttgt attttaatga
     3481 agtattcgac atattccctt cagacggaat atgtcgacgg ttatggtttt actggagcat
     3541 gagtatgtta tggcgaaact gataaatata tcagcgtcca ctgaggagag aatgacgcca
     3601 ggggagagga gggtggcttc acgactggag agctttctga atgatgattg ctttgtctgg
     3661 tacgatatcc cggtgggacg gaaaaaccga catccggatt ttgtcattat tgacccggat
     3721 aacgggctgg tatttctgga ggtcaaggac tggactgtat ccacattacg taaagcaaat
     3781 caggagcagg ttacactgga aaccgacggt cttctgaaat cagaaataaa tccgctggta
     3841 caggttcgtc gttatgcctg tgatacggtt aatgccttac ctgctgaccc gtgcttacgg
     3901 cagaatgacg gtcagtataa ggggcggctg aacctggcct gggcgtatgg tgtggtgttt
     3961 acccgcatta cccgtcagca actgaaggca ctgacaggga ataatgagaa tgcggtggaa
     4021 aagatttttc cttcagcaca gacaatctgt caggatgaaa tgacgcagtc tgttttacct
     4081 gaggtattcc ggcaaaaaat tgccgggatg tttaccacgg ggttcagaac ccgggtaaca
     4141 ccccggatga gggatattct gcgcgcccat ctgtttcctg aagtgaccgt gaaacagaac
     4201 tcacaaatca aaattatgga tattcagcag gagattctgg ccagaaatat tggtgacggg
     4261 catcgtgtca ttcatggtgt ggccggttcc ggaaaaacca tgattctttt gttccgttgc
     4321 ctttacctgg cagaaaccac gtccggtagt tacgccgccc cagtgcaact acccggaggg
     4381 cgttttcaca caggttattg tcgatctcca cccagccatt gcggcagtat tcgttcagag
     4441 cgtcccattg cttcagcaga taggtgaacg ctttcgctgt atccgagtgg cgcgacagcg
     4501 tgttcatctg cccctggagc cactcataca acgactgcat tagcggtacc gttctggctt
     4561 ttctgaccgc cagtcgctct tctgccggac tgccgcggat ctcagcctcg atagcgtaca
     4621 gttccccgat acgacggagc gcttctcccg ttactgtcgt tggatggcgg acatgtacat
     4681 cgtggatctt gcgtcgggca tgtgccatac aagccgcttc ggttgcctga ccgctttcgt
     4741 aaagagcatt gtaacccgca tatgcatcgg cctgcaggat acctctgtag tccgccagat
     4801 gttgctgtgg gtggatgcct ttgcggtcgg gagagtatga gaaccacacc gctgccggca
     4861 tggtcgaacc ggcgttgcga tcgtcacgaa catagaccca cagccgtccg gtacgggttt
     4921 taccctgacc cggctccagt acatttaccg gcgtgtcatc ggcatgcact ttacccggca
     4981 tcagcacata gtgcttcagt tcatcataca gcgggcgaag ctgctctccc atgatgtcaa
     5041 cccagcgccc catcgtattg cggtgcagct ccacgccctg gcgggcatag atttccgact
     5101 gacggtacag cggcagatgc tcggcgaact tagccatgat tatgcgggcc agcagagccg
     5161 gactggcgta actgcgctcg atgggttttg gtggctgcgg agcctgaact atacagtcgc
     5221 accggctgca ggcccgtttt gggcgaaccg tttcgattac cctgaacgcg gtgttgatga
     5281 tatccagttg ttcagagatg ctttctccca gcggtttcag tttaccgctg cagacggggc
     5341 attcggtttc tgccggggag ataacctgcc tgtcacgggg aagtgttgcc ggaagtgctt
     5401 tgcggacggg agagtctgat gttttcggcg ctgtctctcc ggccattgag gtgagttgca
     5461 actgcgcctc accaagcctg ttctggagct cggttatacg cgtttctgcc cgtgcgatct
     5521 tcttttctat cttctcgcgg cttttctcgc tgctgcgacc gaacaacatt ctctgcagtt
     5581 tagcgaccag cgctctgagt gagctgatct cgcgggcata gccggttatt tcaccagaca
     5641 gacggacgat aacagcctgc tgtgcgatca gcagggcctt cagttgctcg atgttgtcgg
     5701 ggagtgtgtt gttcattccc ctgttttatc acgggttata tccggatgcc aggccgttct
     5761 gtccgtttgg gatgttgcca tgcgatcccc tccagtagca tggataactg agctggcgtc
     5821 aggtgcactt tcccttcccg ggttaccggc cagacgaagc ggccccgttc caggcgtttg
     5881 gcgaacaggc ataacccgtc acgatcggcc cacagtattt tcaccatttt gccactgcgg
     5941 ccccggaaga cgaagatatg cccggagaac ggggcatctt tcagcgtgtt ctgcaccttc
     6001 gaagccaggc cgttgaagcc acaacgcata tctgtgatgc cagcgatgat ccagattctg
     6061 gtaccggttg gcagcgttat catcgggtac ctcctttcat ttcgcggatt agcgcccgta
     6121 acatttccgg agtgagaggg tcaaacagtt ttaccacacc tgatttaaga tgcagctcgc
     6181 accgtgggac gtttccggga tcactctcag ggcactcatc aggcttgtta cgccagaagg
     6241 gatttgtaac tggtctggtc ggctctggcg tatcagtcag agccaccggg acaggcatgc
     6301 attcctgtat gtcatcatcg ctcagtaagc cgtcctcgta ctggcttttc cattttaaca
     6361 gcaggttatc attgataccg tgctctctgg cgatccgggc aacaacagca ccgggctgta
     6421 atgcctgttt taccagcctg attttaaatt cacgactgta ggaggttcgt cgttcttttc
     6481 gccatgtgcc ttcgctgatt tgaggctctg ttaattcctt ctttctgttg gcataaagga
     6541 tggcgtcaag ctgagctaat gaaactgaat cgggcaatgg ccatgcgata ccggatgcaa
     6601 taaatcgctg aaaaagcgta tgtattgtgg aatgactgag acctagacgc tgagcgatgg
     6661 cccggatggt cagtttatct tcaaatctta aacgcagagc atcaggcaaa taagaacgga
     6721 agcagggaat atcttttttt gtctgggaat tcatcgttcg tgtccatcta tatagatggg
     6781 cgcgattgtt gccagacagg acaattttca caagacgtcg cagatggggc gcttacacaa
     6841 caatggcgcg agggaaagct gctattacct tcttcagaga gcccccagct acttcctgtg
     6901 actctcgatg cagctgccga acagccagaa tcgctcgcag aggacccgga aaccctcagt
     6961 atcagctgtt gtcaacgacg gatgactatt gcaccgttcg tgac
//
LOCUS       DAZFBR010000185         7003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFBR010000185 DAZFBR010000000
VERSION     DAZFBR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.185"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..73
                     /locus_tag="ACHJZ2_004629"
     CDS             <1..73
                     /locus_tag="ACHJZ2_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570597.1"
                     /translation="IFKALIQLRRFTGFKNVRLYQLH"
     gene            116..676
                     /locus_tag="ACHJZ2_004630"
     CDS             116..676
                     /locus_tag="ACHJZ2_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4570598.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLMPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     STGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            762..1463
                     /locus_tag="ACHJZ2_004631"
     CDS             762..1463
                     /locus_tag="ACHJZ2_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4570599.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLV
                     TVNDYGVNVTSEHAL"
     gene            1516..4038
                     /locus_tag="ACHJZ2_004632"
     CDS             1516..4038
                     /locus_tag="ACHJZ2_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4570600.1"
                     /translation="MNKSVVSISAAMLVLLCQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVAATNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDLTWQHGMTNTLTFNSG
                     SRIADGYQALMLGGVYGSSLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSDDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGITFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDGDAELVDSERQVAP
                     VAGAAVKVIFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            4054..5388
                     /locus_tag="ACHJZ2_004633"
     CDS             4054..5388
                     /locus_tag="ACHJZ2_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4570601.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWANCTNTQIGQTEDGRTALI
                     EFGKINMTDTYFAPAGSLLATTVVPPTNYTSGGATGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFYDAGGPDGLSDVYATWFAFVGLKQTMAGVTLGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTSATTSWCGNNNTDSSGVGFAKPSGTIYNCVQPNAY
                     IQLSGTSGILFGHDEPGEDSSVHWDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAHPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            5385..6074
                     /locus_tag="ACHJZ2_004634"
     CDS             5385..6074
                     /locus_tag="ACHJZ2_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4570602.1"
                     /translation="MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(6220..7002)
                     /locus_tag="ACHJZ2_004635"
     CDS             complement(6220..7002)
                     /locus_tag="ACHJZ2_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4570603.1"
                     /translation="MSFSIEDFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANL
                     LLVDIKEPDNRYLKHLTHEGVEVEFMTIDITKPDASCTIINRCLERFGQLDILVNNAG
                     VCNINRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKIINMCSVLSFHGGR
                     WSPGYAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKG
                     RIPAQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         1877 a         1726 c         1644 g         1756 t
ORIGIN      
        1 cattttcaag gcacttatac agttaaggag atttaccggt tttaaaaacg tcagattgta
       61 tcaattgcat taattaactc gttctatatt aactctaaat ttcaaggata ttaatatgtt
      121 ttctactttc aaaaaagcag ctctgctggc agctattgca ttaccttttt caactatggc
      181 tgcgcctaca gtcacttttc agggtgaagt aaccgatcag acctgttccg taaatatcaa
      241 cggtcaaacc aattcagtag tattgatgcc gaccgtagcc atggctgact tcggtgcaac
      301 tttagctgat ggtcagagcg caggccagac gccgtttacg gtttctgtgt ctaactgcca
      361 ggctccaact ggtgcagatc aggcaatcaa caccaccttc ctgggctacg acgttgacgc
      421 tagcacgggt gttatgggaa accgtgatac cagcagcgat gcggcgaaag gctttggcat
      481 tcagttaatg gattccagca cttctggtaa cccagtaact ctggctggcg cgactaacgt
      541 accgggtctg accctgaaag ttggcgatac cgaagccagc tacgacttcg gtgcgcgtta
      601 cttcgttatc gatagcgctg ctgccactgc cggtaaaatt accgctgtcg cagaatacac
      661 cctgagctac ctctaattaa tctactggct ggcagtaaat gccagccagt atttcttcgc
      721 acaattaata gtgatttttc atggacatgg aaaagataag aatgaacatt tttgcatatt
      781 tactggtact tgtattttcc atgagcatga gcagcagcgc gtttgccagc gtggtaatga
      841 ccggaacccg tattattttc cctggtgacg caaaggaaaa aaccatccag ttgcgaaata
      901 ccagcgatca gccctatatc attaatatcc atgttgagga tgaacgtggt tctgacaaga
      961 atgtaccgtt tatgccaacc ccgcagacat ttcgcatgga agctgccgca ggtcaggcgt
     1021 tacgcctgct ctacactggt aataatttac cgcaggatcg cgagtctgtt ttctggttta
     1081 gtttcagtca actaccttat ctgaataaga atgataaaag tcagaaccag ctcatcctgg
     1141 ccctgactaa tcgagtcaaa attttctatc gtcccagctc gattgtcggt aaatccagtg
     1201 acgcacccaa aaacctgact taccaggtaa aacagaaccg cattgaagtg acgaatccca
     1261 cgggctatta cgtcacaatt cgcgccgctg aactgcttaa taatggtaaa aaagtccccc
     1321 tcgcgaattc ggtaatgatt gctcctcaaa gcacaactga atggacacta ccctctggca
     1381 tcagtgtcgc tcccggtgcg cagatccatt tagtgaccgt caacgactat ggcgtaaatg
     1441 ttacgtctga gcatgcctta taacctcttt ctcctttttt gagcggtatc ctttttcacg
     1501 cacgagtacg tcgagatgaa taaatccgtt gtgtcaattt ctgcggcaat gttggtttta
     1561 ctttgccaac cggtcatggg gagcgaaatc tcacccgcaa caccgtcaga tgaagacaac
     1621 tacacctttg acccgcaact cttccgcggc agcagattta gtcagtcgtc attagcaaaa
     1681 ctgacaacac gtgagtctgt tgcaccgggc aattataaaa tggatatcta caccaacaat
     1741 aagttgtcag gcagttggaa tgtcacgttt aaagaagccg ctgatggtcg cgttctgccc
     1801 tgcctgacgc ctgaagtcgc ggacgcgata ggcctcaaaa caggggaaga taagggggaa
     1861 aaagatcctg tctgtacgtt tgctaaggaa ctcgctcccg gcatcaccag ccagacacag
     1921 ttgtcacaat tgcgcctgga cttatcggtg ccacagagtc aattgattag tcgccctcgc
     1981 ggctatgttc cccccagcga gctggatacc ggagcatcgc tggcgttcat gaattatatt
     2041 gccaactatt acaacgttgc ctattcaggg cagaatgctc atagccagcg ttcgctatgg
     2101 gcatcattta atggtggcat caaccttggt gcctggcaat atcgtcagtt atccaacatg
     2161 acctgggata atgacaaagg gaatcagtgg aacaatattc gtagctattt gcaacgcccg
     2221 ctgcccgcca taaatagcca gttaatgatg gggcagctta tcaccagcgg aagatttttc
     2281 tctggactca gttatcacgg cgttagtctc gcgaccgatg aacgtatgct gccggactcc
     2341 atgcgcggct atgcgccgac tattcgcggc gtggccgcaa caaacgccag agtctcggta
     2401 atgcaaaacg gtcatgaaat atatcagacc accgtggctc ctggcccttt cgagataaac
     2461 gacctatacc ccaccagcta cagcggcgat ctggatgtca ccgttacgga agctaacggc
     2521 gcagtcagtc gtttcagtgt ccccttttca gccgtaccag aatcgatgcg tccaggaact
     2581 tcccgttata acgtggaagt aggtaaaacg caggatagtg gtgatgactc gatgtttggt
     2641 gaccttacct ggcagcacgg gatgactaat acgctgacat ttaacagtgg ttcgcgtatc
     2701 gctgatggct accaggcgct gatgctgggc ggagtctatg gcagttcgct gggggcattt
     2761 ggggcaaacc tcacttggtc ccatgcgcgt gttcccgaaa gcgaagcgca gagtggttgg
     2821 atgtcgcaat taacctggag taaaactttc cagcctactt caaccaccgt ctccctggca
     2881 ggttatcgat actctaccag cggctatcgt gatctggctg atgtgctggg agagcgtcat
     2941 gctgccagca ataaacagtc atgggactcc agccagtggc gtcaacagtc gcgcttcgat
     3001 cttacgttaa gtcagagcct tgcgaattac ggcaacctgt ttgtgtcagg ttcaacacag
     3061 aactaccgtg gcggcaagag ccgtgataca cagcttcagt taggttacag caatagcttt
     3121 agccatggca tcagtatgaa cctttccgtc ggacgccaaa gaatgggcgg ctataaagac
     3181 aattctgatg atatgcagac ggtaacatcc ctttcattct cattcccact tggcggcaat
     3241 ggacctcgtg taccaagtct tagcaacagc tggacccatt caactgacgg tagctcgcaa
     3301 ttacaaagct cgctaaccgg aatgcttgat gaagcacaga ccaccaacta cagcctgaac
     3361 gtcatgcgcg atcaacaata taagcagacg acgcttagcg gaaacatgca aaaacgtttt
     3421 tcacaaacta ccgtcggatt gaacgcatcg aagggccagg attactggca ggcttcaggt
     3481 aacgtacaag gcgcgatggc tgtgcatggt ggcggcatta ctttcggacc ttatctgggt
     3541 gaaacgttcg ccctggtcga agctaaaggc gcagaaggtg caaaagtcta taactccagt
     3601 cagctggaaa ttaatgacag tggctatgcg cttgttccgg cagtaacgcc ctatcgctac
     3661 aaccgtatat ctctcgatcc acaaggaatg gatggcgatg ccgagttggt cgacagtgaa
     3721 agacaggtag caccggttgc gggtgcggcg gtgaaagtaa ttttccgtac ccgtcctggt
     3781 aaagcgttgc tgattaaatc ccgcatggca gatggttcgg aactgccaat gggagccgat
     3841 gtgctggatg agaataatac agtcgtcggt atagccggtc agggggggca aatttacctc
     3901 cgcacagaac agacaaaagg ccacttgtca gttcgctggg gtgaaggtgc taacgatagc
     3961 tgccaattgc cctttgatat cagcgggaag gacagcaata gccctatcat ccgcctgaat
     4021 gaaacctgtc agtcttgata cagagaaaac ccgatgtcta aatcgacttt tttacacatt
     4081 ctgattagct ccatcatatt ggtggcacta atacaatcat ccgcttgggc taactgtaca
     4141 aacacacaga taggtcaaac tgaagatggt cggacagcgc ttattgagtt cggaaaaatt
     4201 aatatgaccg acacctattt tgcgccagca ggttcactcc tcgcgacaac ggtcgtacct
     4261 cccactaact acacatcagg tggcgcgaca ggaagctccg tattgtggga atgtgatgca
     4321 acagatttgc caaacatcta ttttctggtc gctaccaatg gcgacgaccg cgtggggggc
     4381 ttttacgatg caggcgggcc tgatggtctg agtgatgtct atgccacctg gttcgctttt
     4441 gtcggtctca agcagaccat ggcgggcgtg acgcttggtc gttactggaa gaaagtgccc
     4501 atcaccagtt atgccactca gggaactaaa atccagattc gcttacagga tatccctcct
     4561 cttcatgctg agctttatcg catcagtacg ctacctgata catcagcaac aacaagttgg
     4621 tgcggtaata ataatacaga tagtagtgga gtcggattcg caaaaccttc cggtacaatc
     4681 tataactgtg ttcagcccaa tgcctatatt cagctttccg gtaccagcgg cattttattt
     4741 ggtcatgatg agcccggcga agatagttct gttcattggg atttctgggg tgctgataat
     4801 ggttttggtt acggaatgcg ttcggccaat cgactctaca acaatgccac ctgcgttgcc
     4861 cgcagcgcca cgccgttagt attgctgccg acaattgcag aagcacaact gaatgcgggc
     4921 atggaaagta ccggtaattt taatgtccgc gtcgagtgta gtaactcggt tcaatcaggg
     4981 attagcgata ctcagacagc attaggaatc caggtgtctg aaggtgcata tacagcggcg
     5041 caaaaactgg ggattatcaa tagcaacggc ggcgtcagcg ccctggtctc tgataattat
     5101 gacgcagcag agatggcaaa gggcgttggg atctacattt ctaacagtgc tcaccccgat
     5161 acggcgatga cgctggttgg tcaaccgggc atcgcgaagt taacccccgg aggaaatgca
     5221 gcggggtggt atcctgtatt tgaaggggca acattagaag gtgcgactca ccccggatac
     5281 tccagctata gttactcttt tatcgcccgg ttgaagaaac tgccaaatca gacagtcagt
     5341 gcgggaaaag tgcgggcaac ggcttatata ttggtgaaaa tgcaatgaaa tccgttttgc
     5401 tgctcttaac catcttgttc gtacagtcag tggaagccgg catcatgccg gcttcttcac
     5461 ggattattta tcgcgatggc gatcgggaaa agacactgat gctggtcaat accaatgact
     5521 atccggtact ggtgcaaagt tgggtagata atggtgaagg ttccccagaa gctgcttctc
     5581 ccccctttgt ggtgctgcca ccggttttcc gcctggctgc gagacgaacc caggggttgc
     5641 ggattatcta taatcatgac ccacttcccc aggatcgcga atctgcattc tggttaaatc
     5701 tttatgaaat gccgccagaa actccccatc aagatcccac acatttaacg ctggcaatga
     5761 atacgcaact taaaatcttt tacaggccaa aatctgtaac tatgacgccc caagaagcta
     5821 tgcgtaaaat aagttggcat ctgggtaacg acggcagcag tagctatatt gagcttaata
     5881 acccaacacc actgaatatc tctttcacct ccatcagcat tggggctaca aaggtgcagc
     5941 aagaaagcga tatgatgata aaacccttca gtcaacgacg gtactggtta gcaagtaata
     6001 acgttagtgg tgtggttgcg gcgacatatc tcgatgataa tggtgcacag ataaaacaac
     6061 aattagcgca ataatgctct gagtggtatg taaactcatc gcgatggttc aacgctaacg
     6121 aataaccact atacccacct gaagtaccgg aagtggagaa atgacaaaat ctctccactc
     6181 cattttccag tcaaaacacc tcaacgcagc caaaaatcgt taacgaattg aatatccacc
     6241 atcaatgact aatagttgtc cattgacata atctgaagcg gcactggcga ggaacaccat
     6301 tgcgcccata agatcctgtg cccttcccca gcgttgagcc ggtatacgcc cttttattaa
     6361 ctcttttatt ttagggttat tataaataat agcggtcatt tcactcacat aatatcctgg
     6421 ggcgatacca ttaatctgaa tattatattc cgcaaaatca tcggcatacg ctttggtcag
     6481 ccccgctaaa gcatgtttag tcgccgcata ccctggcgac caacgtcccc catgaaatga
     6541 caatacggaa cacatattaa taattttccc ttttctttgc gggacaaata tattcaatgc
     6601 ggcctggctc atatcgaatg cagcattcag gttgagatta atcatcggat cccagtcgtt
     6661 tcgattgaaa tcgataggac gattaatgtt acacacgccc gcgttattga ccagaatatc
     6721 taactgaccg aaacgctcca gacacctatt aataatggtg catgatgcat caggttttgt
     6781 tatgtctatt gtcataaact caacttcaac gccttcatga gttaaatgct tcaaatatct
     6841 attatcaggc tctttgatgt caaccaacaa tagattagcc cctgctttgg ccagagccga
     6901 gcataagcct ctccccagct cacccgcggc cccagttacg atggccacct tgttatgtaa
     6961 tgaaaaaaaa tccaatgaaa aatcttcaat agaaaagctc ata
//
LOCUS       DAZFBR010000186         6875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFBR010000186 DAZFBR010000000
VERSION     DAZFBR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.186"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(678..1484)
                     /locus_tag="ACHJZ2_004636"
     CDS             complement(678..1484)
                     /locus_tag="ACHJZ2_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4570604.1"
                     /translation="MSDSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPNLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSKEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(1485..1790)
                     /gene="ccdB"
                     /locus_tag="ACHJZ2_004637"
     CDS             complement(1485..1790)
                     /gene="ccdB"
                     /locus_tag="ACHJZ2_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO4570605.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1792..2010)
                     /gene="ccdA"
                     /locus_tag="ACHJZ2_004638"
     CDS             complement(1792..2010)
                     /gene="ccdA"
                     /locus_tag="ACHJZ2_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO4570606.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            2553..3152
                     /locus_tag="ACHJZ2_004639"
     CDS             2553..3152
                     /locus_tag="ACHJZ2_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4570607.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            3149..3769
                     /locus_tag="ACHJZ2_004640"
     CDS             3149..3769
                     /locus_tag="ACHJZ2_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570608.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            3766..3966
                     /locus_tag="ACHJZ2_004641"
     CDS             3766..3966
                     /locus_tag="ACHJZ2_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570609.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            4374..5351
                     /locus_tag="ACHJZ2_004642"
     CDS             4374..5351
                     /locus_tag="ACHJZ2_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO4570610.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(5636..6376)
                     /locus_tag="ACHJZ2_004643"
     CDS             complement(5636..6376)
                     /locus_tag="ACHJZ2_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4570611.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(6497..6727)
                     /locus_tag="ACHJZ2_004644"
     CDS             complement(6497..6727)
                     /locus_tag="ACHJZ2_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO4570612.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
BASE COUNT         1726 a         1797 c         1745 g         1607 t
ORIGIN      
        1 acaatttttt agattgtcac actaaataaa aaagagtcaa taagcaggga taactttgtg
       61 aaaaaacagc ttcttctgag ggcaatttgt cacaacacct tctctagaac cagcatggat
      121 aaaggcctac aaggcgctct aaaaaagaag atctaaaaac tataaaaaaa taattataaa
      181 aatatccccg tggataagtg gataacccca agggaagttt tttcaggcat cgtgtgtaag
      241 cagaatatat aagtgctgtt ccctggtgct tcctcgctca ctcgagggct tcgccctgtc
      301 gctcgactgc ggcgagcact actggctgta aaaggacaga ccaaacatca tggttctgtg
      361 ttcattaggt tgttctgtcc attgctgaca taatccgctc cacttcaacg taacaccgca
      421 cgaagatttc tattgttcct gaaggcatat tcaaatcgtt ttcgttaccg cttgcaggca
      481 tcatgacaga acactacttc ctataaacgc tacacaggct cctgagatta ataatgcgga
      541 tctctacgat aatgggagat tttcccgact gtctcgttcg cttctcagtg gataacagcc
      601 agcttctctg tttaacagac aaaaacagca tatccactca gttccacatt tccatataaa
      661 ggccaaagca tttattctca ggataattgt ttcagcatcg caaccgcatc agactccggc
      721 atcgcaaact gcacccggtg ccgggcagcc acatccagcg caaaaacctt cgtgtagact
      781 tcctttgaac tgatggactt atgtcccatc aggctttgca gaactttcag cggtatacca
      841 gcatacagca tgtgcatcgc ataggaatgg cggaacgtat gtggtgtgac cggaacagag
      901 aacgtcacac cgtcagcagc agcggcggca accgcctccc caatccaggt cctgaccgtt
      961 ctgtccgtca cttcccagat ccgcgctttc tctgtccttc ctgtgcgacg gttacgccgc
     1021 tccatgggta ttttcagtgt tgccaccatc gtctgcagct ggctgacgta ccaggagtca
     1081 gagagcggaa ccagccggtg agtctgctga ccggcgggca ttctccccgc cgtcctggca
     1141 gctttttcgg tccgttgttt cagggtcgca agctgcacaa acggatacgg aggcgcaagc
     1201 gaaaaatccc cccgcgtcag cgccagtgct tcattaatgc gtgctccggt gttccacagt
     1261 gtggccagca gcatcttgcg gtgcagattc gggacgtaat ggagcagggc actcacttcc
     1321 ggagccagca gatattttgg cagttcatca tggaccatcg acatctggcg aagtgccaga
     1381 gctgccggat aatcaatggc aacaggcagc gatgcaggct gtccggcaga atacactgcc
     1441 ggtaccatga ctgcagactg gctgtgtata acggagtctg acatttatat tccccagaac
     1501 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
     1561 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
     1621 ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg
     1681 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
     1741 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagtc
     1801 cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
     1861 ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
     1921 tgtgcttacc agaccggaga tattgacatc atatgccttg agcaactgat agctgtcgct
     1981 gtcaactgtc actgtaatac gctgcttcat agcacacctc tttttgacat acttcgggta
     2041 tacatatcag tatatattct tatgccgcaa aaatcagcgc gcaaatacgc atactattat
     2101 ctggctttta gtaagcctta tgtattttac ctttcgttat gttaaccaat aaaaattaaa
     2161 atctgcctta taaaaacaaa gcgtaattac cgcattcccg tttcgtatgg atccgacttt
     2221 atttgaataa acgactttcc ggaagtgtac cacctgagaa aatcatcata tcgctgtttt
     2281 ccgcccctgc gcggcgcatc acagtccaga gatacaacac aacatatagt gccatgggca
     2341 actgaatgag gcactgcatg ctgtgctgac gggatgtaac gctgtgcgga gggagaggaa
     2401 gatccgtaac caccagaaac cggatctgac caggaaaaga gaaagagtgt atgtaagttc
     2461 tgtactgtat gtatgaattg tattgtatgt attgtgtatt tttcataccg ggcatacgat
     2521 ggcgccattc cctgagaaac cggagccaga ccatgacatc cctctccccg gacaccgtcc
     2581 gccgcattga agacgccgcc gccgccctga tcgccgccgg caccccgaac ccgaccaacg
     2641 agcaggtccg tcagcacctc ggcggcggct ccctgtccca catctccccg gtgatgcgcg
     2701 cgttccgcgc ccgccagcgc gagcaggcca cgcccctgcc gccggagctg gcgcagctgc
     2761 tgaccggcca gctggggctg ctgtggcagg cggccgtgaa acaggctgaa gccggggccc
     2821 tggcggcgcg cgagcaggcc gacgacgaca ttgcccgggc cgacaaggag cgggatgagg
     2881 cgctggcgaa cgtggccgcg ctggaaagcg agctggcggt gctgcgcgag gtggtggccg
     2941 aacgtgaccg cctgctgcag gaggtacgcg agctacgcgc ggaggcgctg ccgctgcgcg
     3001 agcaggtggc ccgcctgacc gccaccggcg aacacctggc ggcgcagctg caggacacga
     3061 aggcggagct gaaggaagcc cgggaggacg gccgccagct tcagaccgaa ctgctggctc
     3121 tggcccgcca ggacgggaag gtgaagaaat gagacaggaa agagtattac gtataaaggc
     3181 atttcgacgg gcactggagc ttggtggcgg tgaacgggct gacgggtgtc gtcattttcc
     3241 ccgctggaaa acggaactga ggaatttccc gcacagcagc tgcgatttag ccagcaatac
     3301 tctggcgtac tacctcaaag aacgagagag atgccatccg tgcatcattt ttatggaagg
     3361 gaacgctggc tttcatgaag cggaaaactc aacagttcac gggcatgtca tcgtattgct
     3421 ggacggagag tacatcgatt tgacgctgga tcaattcgac gaatatccgg actacattcc
     3481 ttacgaacct gtcgaatctg acgggcagat tggtaagctg ttgcgtaata tcatgaagca
     3541 tgaggacccg gtaaaaaccc gccagattga tctcgatggt ggcgaagagc tgtatgcctg
     3601 gttacgcgat accgccgacg aagtgctggc agcagatccg gaatggcagg cccgggaacg
     3661 gtcgatagaa gaagcaaggg aagccgctat tacggtgttc ccgtttctgt ctgacgtgca
     3721 aaaaactgag tgcgaacagg gcccggacag tcaggaggct gccagatgaa gatttatcat
     3781 tacactgacc tgaacggcct gaagggcatc atcgaatcag gctcactgtg ggcgacgcac
     3841 ttttcctttc tcaatgacag taatgagctg actcacggca tgaactgcct tgagaatgcc
     3901 ctgcagtacc tgagggacga ctttaacccg aaaaccctta agttcatcga acaggcaccc
     3961 ttataagcgg ttgtgtatgc tgtttccctg gaatgattta tccacatatc cggaggctcg
     4021 atccagtaaa cagatccatg aatgatcaac aaaggatcca ttaaagatcc ccataccgct
     4081 gcaaaccttg tcactcatgg gccgggacca cgatcacata agcagtggca tgttactgat
     4141 aaactgtaac atgctaatga taagctgtat tcagtaatcc atatactgaa gtaagttaat
     4201 gacataaact atggtcagta cgccagactc agctgttaaa tacaggctgc aggtttttct
     4261 tcagtcagtt agcggggctc tgacacacga tttgctgttt attcttttac tgtccacagg
     4321 caggaggctt tctggaaaac gaaaattcag acatcaaaaa actgttcggc gaggtggata
     4381 agtcgtccgg tgagctggtg acactgacac caaacaataa caacaccgta caacctgtgg
     4441 cgctgatgcg tctgggcgtt tttgtaccga cccttaaatc actgaagaac agtaaaaaaa
     4501 atacactgtc acgtactgat gccacggaag agctgacacg tctttccctg gcccgtgctg
     4561 agggattcga taaggttgag atcaccggcc cccgcctgga tatggataac gatttcaaga
     4621 cctgggtggg gatcattcat tcctttgccc gccataacgt aattggtgac aaagttgaac
     4681 tgccttttgt cgagtttgca aaactgtgtg gtataccttc aagccagtca tcacgcaggc
     4741 tgcgtgagcg catcagccct tccctgaaac gcattgccgg taccgtgatc tccttttccc
     4801 gcaccgatga gaagcacacc cgggaataca tcacccatct ggtacagtca gcctactacg
     4861 atactgaacg ggatattgtt cagttacagg ctgatccccg cctttttgaa ctgtaccagt
     4921 ttgacagaaa ggtccttctc cagcttaagg cgattaatgc cctgaagcga cgggagtccg
     4981 cccaggcact ctacaccttt atagagagcc tgccccggga tccggcaccg gtatcgctgg
     5041 cgcggctgcg tgcacgcctc aatctgaagt ctcctgtatt ttcccagaac cagacggtca
     5101 gacgggcaat ggagcagctg cgcgagattg gatatcttga ttacacggag atccagcggg
     5161 ggcggacaaa actcttctgc attcactacc ggcgtccccg gttaaaagca ccgaatgatg
     5221 agagtaagga aaatccgttg ccaccttcac ctgcggaaaa agtcagtccg gagatggcgg
     5281 agaagcttgc cctgcttgag aaactgggca tcacgctgga tgacctggaa aaactcttca
     5341 aatcccgctg aacataaact gtagtcagtg aagagtgttc ctttactgac tacagcttat
     5401 attatcaggt gcagtgagtg gtctgctcac tgcagtttat attcagtttc ctgcagtgct
     5461 gcctgtagct gagctgtcat ctgccggtcc cttacgtgag tcaccccgta acctgatgct
     5521 gaggcattgc tcccttcata aaacatgact tactcactac agcttatata catgctccag
     5581 cttatgttat gtctgttctg ctgaccacag cttatataag gaagcgcgta tgccgtcacc
     5641 tcaggggagg cagtgtacgc aggatctccg cagcatcccg cccgtcacct gtgaaaggca
     5701 ctgccagcgt ggcagccata tccagcgcaa acactctggt ataaacctcc atcgaacgtg
     5761 gatccctgtg acctgccagg gcctggatga ctttccgggg ctggcggtga tagagcatat
     5821 gcatgatata gctgtgccgg aaggtgtgtg gtgtgaccgg aatcgaaaag tgtactccgt
     5881 cagcttcggc ccgtctgaca gcctgcttca gccagttgcg catggtttcg tcggtcacgg
     5941 cccataatgg ttcacgacga cggggccggg tggtgatcat ccagctttcc atctgcctga
     6001 catagcttat atccgtcagc ggaaccaggc ggacttcatc ttttggcggg cgtccgcgtc
     6061 gcgcacgcac tttttcggac aggatccgca caaacggcct tgctccatcc aggtcaaatg
     6121 attccggcgt cagcatccgg gcttcgccaa tacgcattcc ggtattccag agggtggcga
     6181 acagcatatg gtgacgctga tccggcatat agaaaagaag ggcactcact tccggggcca
     6241 gcaggtaggc cggtgtggcc cctgtggaag tggccattct tctcagggag agcgctgttg
     6301 caaaatccac ccccggcgca atgggtaaca ggaagacgcg ttctggtgaa ttctgcaggg
     6361 gaatgacatt gttcatgagt tatttttcac gtagcacacg cactgtttat ggatacaggt
     6421 attctgacct gaacgcccgg atccttccac gacaataaat cgcagcgtgc ggatcctttt
     6481 gtttgcttat cacgcattat tgcgggtaaa accaaatacg taaaggtacg ttttgcagca
     6541 gggatcccgt attaagccag tcgtggcgcg ggctgagggc tggtttgccg tttttgggag
     6601 aattggggta atgccaggac gctaatgatc tatcaatgga tggatgtaag tgacaattga
     6661 aaactgaaaa atgcgctatg tagattctga cagatttatg cacctgaaat acgttcaaaa
     6721 tccacatagc gtaactctga ttttccttac cagaaaaaag agggggaata accagtatct
     6781 atccccccta cacaattgca gaataataaa ttataaaaat aagaactcag gatttatata
     6841 catgattaca tgtaatatta ctgagaaata ttttg
//
LOCUS       DAZFBR010000187         6861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFBR010000187 DAZFBR010000000
VERSION     DAZFBR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.187"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(97..393)
                     /locus_tag="ACHJZ2_004645"
     CDS             complement(97..393)
                     /locus_tag="ACHJZ2_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO4570613.1"
                     /translation="MERTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
     gene            complement(535..750)
                     /locus_tag="ACHJZ2_004646"
     CDS             complement(535..750)
                     /locus_tag="ACHJZ2_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4570614.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            826..1521
                     /locus_tag="ACHJZ2_004647"
     CDS             826..1521
                     /locus_tag="ACHJZ2_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO4570615.1"
                     /translation="MDGSSTERNNKDMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLSDIGVVFKYLGIDNISFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERLVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVG
                     KVIASQWPEETFG"
     gene            2023..2544
                     /locus_tag="ACHJZ2_004648"
     CDS             2023..2544
                     /locus_tag="ACHJZ2_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570616.1"
                     /translation="MAKSNVSVQAFKDFLEELMSLNIMKEATARNLKNSSARLLTVVQ
                     EEEMGDVTQLDVNELAERYINATEPKPSDSSITAYKSRMESAIKKFVAFQSGEEIPYT
                     PIDKESSEEKDLTGEPTKVEGKANALHTYDLPVVLRPESGVTVTIKGIPNDITNEEAE
                     RISSILKVYVRPQ"
     gene            3113..3295
                     /locus_tag="ACHJZ2_004649"
     CDS             3113..3295
                     /locus_tag="ACHJZ2_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570617.1"
                     /translation="MEQTGRLFKQRRLSTTWLKSQITQPHKLWDAMPKQPSQEELRDC
                     IAKVYSGGIYVQKNRI"
     gene            3273..3545
                     /locus_tag="ACHJZ2_004650"
     CDS             3273..3545
                     /locus_tag="ACHJZ2_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO4570618.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVALYAAGYRKSKQLTAR"
     gene            3604..3855
                     /locus_tag="ACHJZ2_004651"
     CDS             3604..3855
                     /locus_tag="ACHJZ2_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570619.1"
                     /translation="MEFHESAICDFRANANSVKPQPIAVLFKTMGAWAVLCFAADDTD
                     ARMAIGQEMEMDPTNDEFIIYGAPSNYLLDTCNIYNKAA"
     gene            4038..4406
                     /locus_tag="ACHJZ2_004652"
     CDS             4038..4406
                     /locus_tag="ACHJZ2_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO4570620.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSLLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            4479..4643
                     /locus_tag="ACHJZ2_004653"
     CDS             4479..4643
                     /locus_tag="ACHJZ2_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO4570621.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            4612..4755
                     /gene="kil"
                     /locus_tag="ACHJZ2_004654"
     CDS             4612..4755
                     /gene="kil"
                     /locus_tag="ACHJZ2_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO4570622.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            4830..5126
                     /locus_tag="ACHJZ2_004655"
     CDS             4830..5126
                     /locus_tag="ACHJZ2_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4570623.1"
                     /translation="MNAYYIQDRLEAQSWTRHYQQIAREEKEAELADDMGKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            5132..5917
                     /gene="bet"
                     /locus_tag="ACHJZ2_004656"
                     /pseudo
     CDS             5132..5917
                     /gene="bet"
                     /locus_tag="ACHJZ2_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            5914..6587
                     /locus_tag="ACHJZ2_004657"
                     /pseudo
     CDS             5914..6587
                     /locus_tag="ACHJZ2_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
     gene            6584..6766
                     /locus_tag="ACHJZ2_004658"
     CDS             6584..6766
                     /locus_tag="ACHJZ2_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO4570624.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            6739..>6861
                     /locus_tag="ACHJZ2_004659"
     CDS             6739..>6861
                     /locus_tag="ACHJZ2_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4570625.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLA"
BASE COUNT         2010 a         1540 c         1611 g         1700 t
ORIGIN      
        1 cctcaggtga atcagggtgt cgaatctctt ctttttcctg gtatttctta cgacgttttg
       61 tcataattac tcctgtggat tgatccagta attccctcag aattgcatat caatttgctt
      121 aaaatcctcg gtggcagccg gggatttttt ctttgtgatt tcatcaagcg catacttaaa
      181 agccctgcta atcggactga tgtctgatgc cattccgaaa gcacacaaga ccgaagcaat
      241 aaaccgccag tccgttctgc ttatcttcga ttcatgacag ccaatcatct ttgccagacc
      301 gcgctgtgta agcgttgaca ggttgatgag taaatctgtt tctgcgcgat caacgtcgcg
      361 ctgtgatagt ttgctgtaac ttgttcgttc catttcttaa gatttccaat agtgaatagt
      421 tagttgaaag gtatgcgtgg aaacgcatat ggccttagtt ggtcagatat cttgggactc
      481 gcttttcagc gacgtaggac gaatgtccgt tgttacaaag agcggctccg cttattaagc
      541 ggctttgtgc tccggcggga acacgtcatc aagacttact tttgcgccta acttgtttag
      601 acacgcaaca agagcacggc atgttttaag gtctgggaag cgacgaccag attcccaatg
      661 tccgatagct ccctgtgtgc atccaactgc cttagcaagt gttgtttgag agatattcag
      721 tgactctcga tattttcgta ggttgctcat atgccctcca tagtaaccac gaataaaaaa
      781 atacaatatg tacttctcaa atacaagtaa aaatacacat tgtgcatgga tggttccagt
      841 acagagcgta ataataagga catgaaaatg aaatggtatg aactggctag atccagaatg
      901 aaagagctcg gcataactca agagaagtta gccgaagagc taggtatgac gcagggtgga
      961 attggacact ggttgcgcgg atctcgtcat ccatctctta gtgatattgg tgtggtgttt
     1021 aaataccttg gtattgataa catatcattc aaccacgacg ggacattttc acctgttggc
     1081 gaatactcat cggccccagt taaaaaacaa tatgagtacc ctgttttttc tcatgttcag
     1141 gctgggatgt tctctccaga actcagaacc tttaccaaag gtgatgcgga gagattggta
     1201 agcacaacca aaaaagccag tgactctgca ttctggcttg aggttgaagg taactcaatg
     1261 accgcaccaa caggttccaa acctagtttt cctgacggga tgttaattct ggttgaccct
     1321 gagcaagctg ttgagcccgg cgatttctgc atagccagac ttggtggtga tgaatttacc
     1381 ttcaagaaac tgatcaggga tagcggtcag gtgtttctac aaccactaaa cccgcaatat
     1441 ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc cagccaatgg
     1501 cctgaagaga cgtttgggta aagaggatag atggagttta atggccatga gtatcaagcg
     1561 gtcaaaaaga agttcatcta ttcattgaaa gcataaggcc atctgagtat atccgtaatg
     1621 atctggatat tgtctataac atcaatgacc ggacgataga tatcggtgag cagcgtcctg
     1681 tatggcaggg tgaggcaggt gaaaaaacgt cctgccatca acaagaatca agtgcatccg
     1741 ctctctggac agatggaaaa tttattggat gcaaaaagat atgaaataac acctgtatag
     1801 cacggagctt tctctgaccg atgcacttga agttgttagg gctgacccag cctgctgttt
     1861 ctttaggtaa gagaaaagag atttaggaga tgaaaggtcg cagaggtgcg gcctttttta
     1921 ttgagagtgg atcttgaacg gaaattctca agacttgagt cttgcatgca aatcaattcc
     1981 tggataaact cgatctgagt caataactta gagagagagc aaatggcaaa gtcaaacgtt
     2041 agcgtgcagg cattcaagga cttccttgaa gagcttatgt cgctgaacat aatgaaggag
     2101 gccaccgctc gaaatttaaa aaactcatcc gctcgcctct taacggtagt ccaagaagag
     2161 gaaatgggtg atgttactca gcttgatgtg aatgagcttg ccgagcgata catcaacgca
     2221 actgagccga agcctagcga cagcagcatt actgcatata aaagccgcat ggaaagtgca
     2281 atcaaaaagt ttgtagcttt ccagtctggt gaagaaatcc catacactcc gattgacaaa
     2341 gaatccagtg aggaaaaaga tttgactggc gaaccaacaa aagtcgaagg caaggctaat
     2401 gcacttcata cctatgatct tccagtagtt cttcgacctg aatcaggggt tacagtaacg
     2461 attaaaggca ttcctaacga tatcacaaac gaagaagccg aacgcatctc ttcaattctg
     2521 aaggtttacg ttcggcctca ataatgcaaa agcattcagc acaatgtcca actccccagt
     2581 ccgacattga tgctgtttaa ccagagcctc caaaaggagc ccttgttaag gtacacaata
     2641 tttgcgatgt aaccttagcg cgtctggtac attttttcaa gcggttgtag ggctgccgcc
     2701 aatatgagaa aacacagatg tctacataca atttgaatga ccaattcgat cgagaagttc
     2761 atgtgaatgc ctatgaacgg ataaggcatg gaaatcttga acatgtgtgt gagcattatc
     2821 gctcaaggcc acaccgctaa tcatcaaccc ggcctccatg ccgggttttc ttttcctctc
     2881 gcccaaaaaa acacataacc aattgtattt attggaaaat aaatagatac aactcactaa
     2941 acaacgcaat tctgatctct ccttacatcg ccgaggcaat acacccacgc taaaaaacaa
     3001 tactattaaa tacaaagcgt tgtaaaaaaa cgccccgttt tagaacaaat tgtattgacc
     3061 aagtgaagta catatcgtac tatttaacca tcagcaggac gctggaagcc aaatggaaca
     3121 gactggcagg ctctttaaac aacgtcgact ctcgactacg tggctgaaaa gccagatcac
     3181 ccaaccacat aagctgtggg atgcaatgcc gaagcaaccg tctcaggagg agcttcgaga
     3241 ttgcatcgcc aaagtttatt cgggaggaat ctatgtccag aaaaacagaa tttaaaggca
     3301 ccgcagcttc tcgccgtaga gctcgtcgcg caaacctgca aagtcaggag gcgattagct
     3361 ccgacaaact acacaggcca accccatcac gagtggtctt acaatgcaaa cgcaaaccag
     3421 caatgagagc agaagtaata acactgacaa cgttgaccag aaaatatgaa ggctcaactt
     3481 gtcttccaaa tgtagctctt tacgcggcag gctaccgtaa atccaaacaa ctgacagcaa
     3541 gatgacttgt gttggtcgcc agaaaatgaa attaggcagc aaaccactta tttgaggtga
     3601 tatatggaat ttcatgaaag tgcgatttgt gattttcgcg ctaacgcaaa ttcagtaaaa
     3661 ccacagccaa ttgcagttct ttttaaaaca atgggtgcgt gggctgtttt atgcttcgcc
     3721 gctgacgaca ctgacgcaag aatggcaata ggacaagaga tggagatgga cccgacaaac
     3781 gatgaattca taatttatgg cgctccatct aattacttac ttgatacctg caacatttac
     3841 aacaaggctg cctgatggtg gcctttattt ttggcataaa caacagaata aacacagcac
     3901 tgtgtattca ttccaacgag tgaatacacg gagcaatgtc gctcgtaact aaacaggagc
     3961 cgacttgttc tgattattgg aaatcttctt tgccctccaa tgtgagggcg attttttatc
     4021 tgtgaggata tgaacagatg tcaaacatca aaaaatacat cattgattac gactggaaag
     4081 catcaataga aattgaaatc gaccatgacg taatgacaga ggaaaaactt caccagatta
     4141 ataatttctg gtcagactct gaataccgac tcaataaaca cggctcttta ttaaatgctg
     4201 tattaatcat gctggcgcaa catgctctgc ttatagcaat ttcaagcgac ttaaatgcat
     4261 atggtgttgt gtgtgagttc gactggaatg atggaaatgg tcaggaagga tggcctccaa
     4321 tggatggtag cgaaggaata agaattaccg atatcgatac atcaggaata tttgattcag
     4381 atgatatgac tatcaaagcc gcctgagcgc ggcgttaccg cataccaata acgcttcact
     4441 cgaggcgttt ttcgttatgt ataaataagg agcacaccat gcaatatgcc attgcagggt
     4501 ggcctgttgc tggctgccct tccgaatctt tacttgaacg aatcacccgt aaattacgtg
     4561 acggatggaa acgccttatc gacatactta atcagccagg agtcccaaaa aatggatcaa
     4621 acacttatgg ctatccagac taaattcact atcgccactt ttattggcga tgaaaagatg
     4681 tttcgtgagg ccgtcgacgc ttataaaaaa tggatattaa tactgaaact gagatcaagc
     4741 aaaagcattc actaaccccc tttcctgttt tcctaatcag cccggcattt cgcgggcgat
     4801 attttcacag ctatttcagg agttcagcca tgaacgctta ttacattcag gatcgtcttg
     4861 aggctcagag ctggacgcgt cactaccagc agatcgcccg tgaagagaaa gaggcagaac
     4921 tggcagacga catgggaaaa ggtctgcccc agcacctgtt tgaatcactc tgcatcgatc
     4981 atttgcaacg ccacggggcc agcaaaaaag ccattacccg tgcgtttgat gacgatgttg
     5041 agtttcagga gcgcatggca gaacacatcc ggtacatggt tgaaaccatt gctcaccacc
     5101 aggttgatat tgattcagag gtataaagcg gatgagtaca gcactcgcaa cgctggcagg
     5161 gaagctggct gaacgtgtcg gcatggattc tgtcgaccca caggaactga tcaccactct
     5221 tcgccagacg gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat tgttgatcgt
     5281 cgccaaccag tacggcctta atccgtggac gaaagaaatt tacgccttcc ctgataagca
     5341 gaacggcatc gttccggtgg tgggcgttga tggctggtcc cgcatcatca atgaaaacca
     5401 gcagtttgat ggcatggact ttgagcagga caatgaatcc tgtacatgcc ggatttaccg
     5461 caaagaccgt aatcatccga tctgcgttac ctagtggatg gatgaatgcc gccgcgaacc
     5521 attcaaaacc cgcgaaggca gagaaatcac cggcccgtgg cagtcgcatc ccaaacggat
     5581 gttacggcat aaagccatga ttcagtgtgc ccgtctggcc ttcggatttg ctggtatcta
     5641 tgacaaggat gaagccgagc gcattgtcga aaatactgca tacactgcag aacgccagcc
     5701 ggaacgcgac atcactccgg ttaacgatga aaccatgcag gagattaaca ctctgctgat
     5761 cgccctggat aaaacatggg atgacgactt attgccgctc tgttcccaga tatttcgccg
     5821 cgacattcgc gcatcgtcag aactgacaca ggccgaagca gtgaaagttc ttggattcct
     5881 gaaacagaaa gcctctgagc agaaggtggc tgcatgacac cggacattat cctgcagcgt
     5941 accggaatcg acgtgagagc tgtcgaacag ggggatgatg catggcacaa attacggctc
     6001 ggcgtcatca ccgcttcaga agttcacaac gtgatagcaa agccccgctc aggaaagaag
     6061 tggcctgaca tgaaaatgtc cacaccctgc ttgccgaggt ttgcaccggt gtggctccgg
     6121 aagttaacgc taaagcactg gcctggggaa aacagtacga gaacgacgcc agaaccctgt
     6181 ttgagttcac ttccggcgtg aatgttactg aatccccgat catctatcgc gacgaaagta
     6241 tgcgtaccgc ctgctctccc gatggtttat gcagtgacgg caatggcctt gagctgaaat
     6301 gcccgtttac ctcccgggat ttcatgaagt tccggctcgg tggtttcgag gccataaagt
     6361 cggcttacat ggcccaggtg cagtacagca tgtgggtgac acgaaaagat gcctggtact
     6421 ttgccaacta tgacccacgt atgaagcgtg aaggactgca ttatgtcgtg gttgagcggg
     6481 atgaaaagta catggcgagt tttgacgaga tggtgccgga gttcatcgaa aaaatggacg
     6541 aggcactggc tgaaattggt tttgtatttg gggagcaatg gcgatgaagc atcctcacga
     6601 taatatccgg gtaggtgcga tcactttcgt ctactccgtt acaaagcgag gctgggtatt
     6661 tcccggcctt tctgttatca gaaatcccct gaaagcacag cggctggctg aggagataaa
     6721 taataaacgg ggagctgtat gcacaaagca tctcccgttg agttaagaac gagtatcgag
     6781 atggcacata gcctcgctca aattggagtc aggtttgtgc caataccagt agaaacagac
     6841 gaagaatttc atacgttagc c
//
LOCUS       DAZFBR010000188         6785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFBR010000188 DAZFBR010000000
VERSION     DAZFBR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.188"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(179..664)
                     /gene="sixA"
                     /locus_tag="ACHJZ2_004660"
     CDS             complement(179..664)
                     /gene="sixA"
                     /locus_tag="ACHJZ2_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO4570626.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(867..3011)
                     /gene="fadJ"
                     /locus_tag="ACHJZ2_004661"
     CDS             complement(867..3011)
                     /gene="fadJ"
                     /locus_tag="ACHJZ2_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO4570627.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3011..4321)
                     /gene="fadI"
                     /locus_tag="ACHJZ2_004662"
     CDS             complement(3011..4321)
                     /gene="fadI"
                     /locus_tag="ACHJZ2_004662"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO4570628.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4501..4785)
                     /locus_tag="ACHJZ2_004663"
     CDS             complement(4501..4785)
                     /locus_tag="ACHJZ2_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO4570629.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5157..6497
                     /gene="fadL"
                     /locus_tag="ACHJZ2_004664"
     CDS             5157..6497
                     /gene="fadL"
                     /locus_tag="ACHJZ2_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO4570630.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1697 a         1833 c         1604 g         1651 t
ORIGIN      
        1 aaaatacatt acaacttagt ctcatttcgt cagaaattaa tagggaagca gaggaaaaag
       61 tgcgatgaat tctgtatcga agcgtatcaa ctccgtgaag ggttttgagt gaatttctac
      121 tattgcaaaa agacaaatgc ccgatgtaca gcttacatcg ggcatttttt caaacgaatc
      181 agatagcttt cgccatcttc aaattacatg ggctcatctg ccagttgaac tcgccttttc
      241 cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc
      301 ccggacataa ctcggcgacc agatacccaa ctaacggtaa gtgggagata accaacaccg
      361 aagcaacacc ttcattggtc aacgcctgca aataggcact gaccagacca acatcgccgc
      421 agggcgttaa ctccggcaga acttctgcac tggaaggcag gttcagacaa tccccaactt
      481 cctccagtgt ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg atttccactt
      541 tttgaccttt cagccagttc gccatcaggc gagattcgtc acaaccgtta gtggtcagag
      601 gacgaaccga atcactggcg gcatcgaggg ctgcgtcgcc gtgacgcatg ataaaaactt
      661 gcatattgca ccgcttttgt taaccagatt cgcccgtttt ctttcatctg aaaccagaga
      721 aagaaaacgg tggccggcat tgtgccttat ccattcaccg aatgaaacgc tgttttttac
      781 ctcaatggcg taagtatagt caatatgcgt ttacattttg accaacaatc cgccattcag
      841 cgcggattca tatagctttg accttcttat tgcaggtcag ttgcagttgt tttccaaaaa
      901 ctttccccac gcgcacccat ctcgactaaa cgctcgcaag gggtaaaacg tgaaccatac
      961 tgcgtggcca gtcgttgcat tattgcaacc acttcgcccg cgccgagaga atcgatatag
     1021 cgaaacggtc caccgagaaa tggcggaaaa ccaatgccaa ataccgcacc aatatccccg
     1081 tcacgcacgc tacggataac ctgctcatca acacaacgta cagcttcatt cagcatcaac
     1141 atcacacacc gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg
     1201 agcgggtaaa tggcaggatc gacctgtttt ttgcttttac gccctttctg accataaaga
     1261 tagaaacctc gaccattttt tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca
     1321 tttgcaggcg cgctaaaacg ttctccataa gcggcttcca gtacaggaat aattttagtc
     1381 ccggtgtcga ttcctacctc atccaaaagt tggattggac ctaccggaaa accaaatttc
     1441 actagcgcgg catcaatgtg cgctacccgt tcaccttcgg tcagcatgcg gatagcttca
     1501 ttaatgtaag gcgctaagat gcgattgacg taaaacccgg ctttgtcacg cacgacaatg
     1561 ggcgttttgc cctgtttttt cgccagtttt accgtagtgg cgatggtttg tgctgatgta
     1621 cccgcatgag gaataatctc caccagcggc attttttcca ccggactgaa gaaatgcagg
     1681 ccgataactt gctcaggtcg cgtggcgtga gcggcgatat caccaatcgg taaagatgac
     1741 gtattcgaag caaagatggt atgagcggcg caattttgct caacttccgc caccatctgt
     1801 tgtttcaatt cgagattttc aaacaccgct tcaataatca gatcgcgatg ggcaaagccg
     1861 cgatagtccg tcgttccgga gattaatgcc agctgtttgt cacgttcgct ggctttgaga
     1921 tgacgacggc gaactttgcc ctccagctga tcccaactgt acttcagcgc atgatttatg
     1981 ccctgcgggt tgatatcttt aattctgacc ggaatccccg ctttacaagc agtgacataa
     2041 gcaataccgc cgcccatcaa gccaccacct aaaatcccca cgctgtttaa tggcgcaggc
     2101 ggcgcatcac tgccgggatc tttcttcact tccgtactgg caaaaaagat actacgcaaa
     2161 gcttgcgatt gtggcgtcat cgccagttcg ccaaacgccc gtgcttcagc gtcatagcca
     2221 ctgctggtgc cctgcgctaa tcccgtttca acaacctcca ggatgcgttc tgtcgccgga
     2281 taattgcctt gagttttgtg ttctgttttc ttgccgacca ttttgaacag cagcgcacga
     2341 cctaacggcc ccgccagaat acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc
     2401 ttctttgcca gctcaacagc ggcttccagc agaatggagt gcggaacaac atcatccacc
     2461 agccccagct ttaatgcctg tttcgcccga agttgttttc cggtgaggat catctctaat
     2521 gctgtgctga cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat
     2581 ccaagttgta cttcaggcaa accgagcacc gttttaggat cgtcagtaca aacgcgaccg
     2641 tggcacgcca gcgccaactc cagcccacca cccaggcaag cgccatgaat agccgcgata
     2701 accggaatgg gcaaagcatg aatctccgcc atcaactgtt ggccctgccg cgccagagct
     2761 tccgcttctt gcgccgtttt gcaattgccg atcatgttga tgtctgcgcc agcaatgaag
     2821 ttgtccggtt tagcggagac aaacaccacg cctcgcaact ctttgttttc acggagttgc
     2881 ttaataatgg cgcgcacctg cgaggcaaac tccgccttca gggtattcat tttctcaccc
     2941 ggtacgtcga tggtgatgat agcaatgttg tccagacgaa cattaagagt aaacgctgat
     3001 gtcatttcca ttattccgcc tccagaacca ttgccgcgcc aagcccaccg gcagcacagg
     3061 cggtaactaa accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca
     3121 tccgcgcgcc ggtcgccgcg aagggatgcc cgtaagcaat cgaaccgcca agcacgttaa
     3181 atttgctatc gtccacttcg ccagtggcat gtgcacgccc cagcgcttca cgagcaaaac
     3241 gttcactacc cagtagctga atattggcca acgtctgggc ggcaaaggct tcgtgcatat
     3301 caatcaatgt cagatctgcc attgtcaaac cggcacgctc cagcgccagc ggtgttgacc
     3361 aggctggacc gagcaacatg tcctgccaga catcgatagc ggtaaatgcg tagctgcgca
     3421 gatatcccag cggtaccagc cctaattctt tcgcccggga ttcagtcatc aggatcaccg
     3481 ccgctgcgcc atcggtcagc ggcgtactgt tcgccgccgt taccgttccg tgtttacgat
     3541 caaacgccgg acgcagcttt gcgaaatcag caagcgagga attaccgcga atattgttgt
     3601 cttccgcgag cagttgttta taaggaggga taaaggcagt catcacctct tctttgagtt
     3661 ttccgtctga ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct
     3721 gttctcgggt gatgccgtag gttttcgcca tttgctctgc agtgtcgccc atccgcaagc
     3781 cggtagaata ttctgctacc gcaggtggta cgggcattaa gtcgcgcaaa cgcaggcgag
     3841 agaagagttt cagacgctgg ctcattgtgc gggctttgtt gacatcaacc agcacgcgcg
     3901 ccagtttttt actgacgcca attggcaata ccgaagagga atctgcccca ccggcaatcc
     3961 ccgctcggat agtccccgcc atcaggcttt ctacgacgtt tgcaaccgcc tggaaactgg
     4021 tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac attcattcca gtaccgagaa
     4081 caatttcacg cgcaatgttg ggagcttcag gcatttgtac gacctgacca aagaccagtt
     4141 gttcaatcac ttcggcgggg atctcgctgc gtgccagcag ttcgcctacc accatcttcc
     4201 ctaaatcaac cgcgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta
     4261 aaccgctaac aatggcgata cgatcgccct ggcgggtaac cagcggtaaa acctgaccca
     4321 taacactccc ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt cttaaccatt
     4381 tttttacatt tagccaagcg gagaaaaggg aaattgggag ctatgacaca gtgaagacag
     4441 gagaaagaaa agaaaacgcc cctacctgtt gctggcagga gcgtacttat ccgagaggaa
     4501 ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag tgaaatcgat
     4561 atccaggcgc acaccatcag actcttcagt gaaagtcggg gagattttgc acggttcgga
     4621 ttccacgcta cgggctttct cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt
     4681 tgcaaacacg cggctgtaag acgctgtgca gtcggagttg tccataatgg tgccaacatc
     4741 catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg atttcctctg
     4801 tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg gaaagtgctg
     4861 ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta aaaatgatct
     4921 aaaacaaaat tcacccgaat ccatgagtgc gccacctcca aattttgcca gctggatcgc
     4981 gtttcttaga tcatatttga aaaaagatag aaacatactt gcaacattcc agctggtccg
     5041 acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta cacttcgcgc
     5101 tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt atggtcatga
     5161 gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt atctccaccc
     5221 aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg ggccgggctt
     5281 attcagggga aggcgcaatt gccgatgatg caggtaacgt cagccgtaac cccgcattga
     5341 ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgac ccggatgtaa
     5401 atatcagcgg aacgtctcca tctggtcgta gcctgaaagc cgataacatc gcgcctacgg
     5461 catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt tggggcgctt
     5521 ctattacctc taactatggc ctggcaacag agtttaacga tacttatgca ggcggctctg
     5581 tcgggggtac aaccgacctt gaaaccatga acctgaactt aagcggtgcg tatcgcttaa
     5641 ataatgcatg gagctttggt cttggtttca acgccgtcta cgctcgcgcg aaaattgaac
     5701 gtttcgcagg cgatctgggg cagctggttg ctggtcagat tatgcaatct cctgccggga
     5761 agactcctca agggcaagca ttggcagcta ccgccaacgg tatcgacagt aataccaaaa
     5821 tcgctcatct gaacggcaac cagtggggct ttggatggaa cgccggtatc ctgtatgaac
     5881 tggataaaaa taaccgctat gcactgacct accgttctga agtgaaaatt gacttcaaag
     5941 gtaactacag cagcgatctt aatcgtgtgt ttaataacta cggtttgcca attcctaccg
     6001 ccacaggtgg cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag
     6061 tgtcgggtta taaccgtgtt gatccgcagt gggcgattca ctatagcctg gcttacacca
     6121 gctggagtca gttccagcag ctgaaagcga cctcaaccag tggcgacacg ctgttccaga
     6181 aacatgaagg ctttaaagat gcttaccgca tcgcgttggg taccacttat tactacgatg
     6241 ataactggac cttccgtacc ggtatcgcct ttgatgacag cccagttccg gcacagaatc
     6301 gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg acttacgcgt
     6361 ttaataaaga tgcttcagtc gacgttggtg tttcttatat gcacggtcag agcgtgaaaa
     6421 ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc ggtactaact
     6481 ttaactacgc gttctgataa cgcgttcgcc tggataaagt cacctgctat gcaggtgact
     6541 ttaactcccc cacttcaccg aaagtagttt cctcattgct tgcctcctga gttttgcaaa
     6601 cagcctgatg gcagctagcc ctttcaaatc agtaagtggt atccacagac aaacctgaag
     6661 ggaaacgcat attttcaggc gttctgctcg tccttctcaa agagtttact tttctgaatt
     6721 tccaggatac tcaaccccct ggctattgtg cgctcataca cgcaaattaa agataggttc
     6781 taaat
//
LOCUS       DAZFBR010000189         6776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFBR010000189 DAZFBR010000000
VERSION     DAZFBR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.189"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            98..805
                     /locus_tag="ACHJZ2_004665"
     CDS             98..805
                     /locus_tag="ACHJZ2_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4570631.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            802..2229
                     /locus_tag="ACHJZ2_004666"
     CDS             802..2229
                     /locus_tag="ACHJZ2_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO4570632.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGCKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2239..2793)
                     /locus_tag="ACHJZ2_004667"
     CDS             complement(2239..2793)
                     /locus_tag="ACHJZ2_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4570633.1"
                     /translation="MNKKLMYIFTIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            2895..3602
                     /locus_tag="ACHJZ2_004668"
     CDS             2895..3602
                     /locus_tag="ACHJZ2_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4570634.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYERAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            3599..5050
                     /gene="djlC"
                     /locus_tag="ACHJZ2_004669"
                     /pseudo
     CDS             3599..5050
                     /gene="djlC"
                     /locus_tag="ACHJZ2_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(5110..>6776)
                     /gene="hscC"
                     /locus_tag="ACHJZ2_004670"
     CDS             complement(5110..>6776)
                     /gene="hscC"
                     /locus_tag="ACHJZ2_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO4570635.1"
                     /translation="NAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIISMD
                     ENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSLKE
                     DAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGLHT
                     QQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRADV
                     ARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYENELEDLWLPLLNR
                     LRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIVAL
                     GAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRVET
                     YSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGLLE
                     VDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEELW
                     ARALGDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEIP"
BASE COUNT         1753 a         1545 c         1648 g         1830 t
ORIGIN      
        1 ttattttatg cgtattatgt cgcgtcagaa aagtataaat aaacgagcgt cgcaatgcgg
       61 ctaatattca ttcaatgaat atttaaggat aaatcatatg gacatggagt cgcaaaaaat
      121 attgtttgca ctttccactc cgatggaaat acgcaatgaa tgctgtttac cttcgcattc
      181 atcacccaaa atgtatttag gtacacgctt ttttgacctt tcttcctcct ggggaattga
      241 tgaccgtgat gacctgctgc gcacaattca ccgaatgatc gataacggtc atgccgcgcg
      301 gttggcaggg ttttatcacc gctggtttcg ttattctcca tgtgaatggc gtgactatct
      361 tgctgaatta aatgagcaag gtcaggctta tgcgcaattt gtcgccagca cagccgaatg
      421 ctgtggtgag ggaggaatta aagcatggga ttatgtccgg atgggttttt taagtcgaat
      481 gggcgtactt aataactggt tgagtgaaga agaaagtcta tggatccagt cgcgtattca
      541 tctacgggct ctacgttatt atagtaactg gcggcaatac tttgccggtt atacctttgg
      601 tcggcaatat tggcagtctc ccgaagatga taatctgcct ttattacgtg aattcttagc
      661 gcgtaaagaa tacgacgatt ccggcaatga tatgttttat caattatttg ccagtgacga
      721 tgcatattac gcgaccttac cctggcaacc actggctgac taccccacat gcccggaaac
      781 gcttaaggat atgagcgacc tatgaaaaat tgctggaaga tcctcaacat agaggaaacg
      841 actgatgtcg atattatccg ccgcgcttat ctggcgctgt taccgtcctt tcatccagaa
      901 accgatccgc agggttgtaa acaacttcgt caggcgtatg aggaagcgct ccggattgcg
      961 cagtcgcctg ctaaatctgt ttggcaacca gaagaatatg aggtagcaga acatgaaatt
     1021 ctgctcgcct ttcgtgcgtt acttgcctct gatagtgaac gttttctgcc ctccgcctgg
     1081 cagcgattca ttcagcaatt aaattattgc tcgatgaatg agattgatga attacgctgg
     1141 tcgctgtgca caatagccat gaacactgcc catttatcct tcgagtgcgt ggtgttatta
     1201 gcagaaagat tgcggtggtt gcaggaggaa aacgtcgggg aaatagacga agaagaactg
     1261 gaatcctttt tatatgccat tgcgaaggga aatgttttta acttccagat cattctgcat
     1321 ctgcccgttg ccgtgcaaaa tgacaccatc gatttttacc aaatgttcgc ccggatttgg
     1381 tcatcgcatc cagaatggct gacattgtat ttagcgcaac atcgcgcagt gattatcccc
     1441 gatgatgcaa aactgcacag aaatttactc cgctggtata gtgcaggtcg cctgggaatc
     1501 cccgaactcc tggattacgc ccggtcgtgg cgggaagctg aacctgataa tgaagatgcg
     1561 cgttattatg aatacgcgca acgcgtctat tgtggagaag gcgaaagcct gctggcagaa
     1621 ctttgtgact actggcacga gtatccctcc actcaggcgg atgctttaat gttgcaatgg
     1681 tgccgtcagc atcgagtcga ttattaccca ttagtggtga tgatgattga agctcgtgaa
     1741 ctggttaacg accagggaaa accgctactt tatgtccccg gcgacagcgc ccgtacgcgt
     1801 tttcatttat acgaaatact cagcgatgaa aaactctctg ctctggggcg ttcactggtc
     1861 gagatggttt tgcacaaagg atgtaagccg cggatctcac tcacgcgtga tacagaacat
     1921 accttatggc cattatatct agttgccaaa caattagtgc aggccagcca acctacagaa
     1981 gaatcattaa tgccgatcgt aagccgtctt gatgcagaaa atcgttgtcc actggaagca
     2041 ttaattattc gtcgattatt aattcaggcg gcgaatttta ccgagaagca aactgttgaa
     2101 ccggagccgc aaccgcagcc aatgcccgtt gacgatggtg ggccaggctg tctgggcatc
     2161 attaaaatta ttttctatat ttttatcttt gctggtttga tagggaaaat actccatctg
     2221 ttcgggtgac agtcgacgtt aatgtgcggg aagcccggca agtaatcttt ccgcattttt
     2281 gtaatgttgt ttggcggcca gttttaacca tttttgagat tcataatggt taacattaat
     2341 acctgagcca tttctgtaga tcaaacctaa agtatattga gcatgagcat acccttgttc
     2401 agctgcttcc ttataccaga gaattgcttt acgggtgtcg ggtttgaccc ccagaccttt
     2461 gaggtagatc caccccagtt tactttgcgc gcgtggattt tcctgttccg ctgattgctc
     2521 ataccagtat ctcgcctgtg tataatccag tggtttaccg tcgctaccat gaagataaaa
     2581 ttctccaagg cgatattgcg acgttgagtg accatgaatc gcggaaagct ccgtggctgc
     2641 cgtggtgaat ggaagactgc ccggtgagct taaataatca aaggcataat tcaccatggc
     2701 tttatcacgc aacgtcgttt tcttgggttg gctaatacag gtaattgctg caactataaa
     2761 aattgtgaat atatacatta attttttatt catgtgtgaa tgcttcgatt ttctttttat
     2821 tttgaaagcg tcaagtttat actcatcaat aataaaaaag tagtcagtta atgcacttaa
     2881 gggattatta acaaatgaat aaggaagaac aatatcttct ttttgcgctt tctgcgccca
     2941 tggaaatact taatcagggt tgcaaacccg cgcatgactc gccaaaaatg tataccggga
     3001 taaaagaatt cgatctctca tcatcctggg gaataaataa tcgtgatgat ttaattcaga
     3061 ctatttatca gatgactgat gatggtcatg ccaatgattt agctggctta tatctgacat
     3121 ggcatcgctc ttcccctgaa gagtggaaag cattaattgc tggtggctcg gagagagggt
     3181 tgatttatac ccaattcgtt gcccaaaccg ctatgtgttg tggggaaggg ggaatcaaag
     3241 cttgggatta tgttcgaatg gggtttctgt cacgggtcgg tgtgctcaac aagtggctga
     3301 cggaagacga gagtttatgg ctgcaatcgc gcgtctatga acgagctcac cattactatc
     3361 acagctggat gcactatttt tccgcctatt cactagggcg tctctactgg caatcttctc
     3421 aatgcgaaga caacgcttcg ctacgcgagg cgctaaccct gtacaaatac gatagcgccg
     3481 ggagtcggat gttcgaagag ctggctgcgg gaagcgatcg tttctatgcc acgcttccct
     3541 ggcagccatt aactgtccag cctgaatgcc cggtaacgct taaggatgtg agcgacctat
     3601 gaaaacatgt tggcaaatcc tcgaaattga aagcacgacg caaatagaca ttatccgcca
     3661 ggcttatctt gctcgcttac cgttatgtca tcccgaaacc gatccgcaag ggtttaaagc
     3721 attacgccag gcctatgaag aggccctgcg actggcggta aatcctgtcg aggaagcaga
     3781 tgatgaagaa aaagatgatg ccactgagca tgaaatacta cgtgtattca gggcattact
     3841 ggattcagaa agtgatcgtt ttcagccctc cgcctggcag aaatttattc agcaattaaa
     3901 tacctggaac atggaggatg tcgatcaatt acgctggccg ctgtgtgcaa tcgctataga
     3961 agcgcgatat ctttcattaa attgtgcttc tttgctggca gagcgtttga actggcattc
     4021 gtttaatgac agcgaaggaa tagatgagta agaaagggag gcttttcttg aggccattca
     4081 ggctggtgat tgtttcgatt tccttggcct tctggtatat cccgttgcgt tgcagaacca
     4141 gactgttgag tattacttcg cgctggaacg ttgctgccgt taccatcctg actatgtcac
     4201 tgcgtttttg gcgatggaag gtccgtggtt cattcctgat gatgcaaagt tacatcgcaa
     4261 actgttgcgc tggtacagct cggtgcaaac aggtatggcg gaactcattc ctgtagccga
     4321 acagtggcaa atggaagaac cagaaagcga agatgcccgg tattaccagt gtgcacaacg
     4381 tttgtactgc ggcgaggggg aaagcctgct taccgatctc tgcgcgtact ggggaagtta
     4441 cccatctaca caagccgata atttgttgtt gcagtggagc gcacaacatt gcccggatta
     4501 tttcgcgtta ttagtgatgg ttatcgaagc gcggagcatg gtagatgcgc aaggtaaacc
     4561 gttgaaatat gttcctggtg agagcgcccg gacgcgtctg ttatgggcgg aaattttaca
     4621 tagcggaaaa ctatcgccgt taggtcaatc gtttattgag tcgctattct tcaaacgcaa
     4681 agcatgggcg tggtggaaat cgagagtcgg tagcgagaca gaagaagatt caccgttact
     4741 ggatttgtat cgggtagcgg aacaggtagt acttgaagcg tttccgaaac aagagatgct
     4801 ggcccgtctt aatacaaggc tggaaggcgg agatgctcat ccattagagg ccattatcac
     4861 ccggatgctg ttgacgaaag taaaactcga gccggaggat gaagatgtcg atgagccaac
     4921 acctgaaaac catgaagaaa aaaatgatga ggatgaaaaa ccacagagca ttaccagcat
     4981 tatcaaaatc agcttaacgg tgctggtgat aggttatgct ctcggcaaaa tcgcgatgtt
     5041 gtttagttga cgatgtgttt tttgcgccga tgccggatgc agcatttacg ccgcatccgg
     5101 cagccgtgat tatgggatct caatggctaa ataaacgctc gcctgccgcc tgacctcatc
     5161 gacgcgggcc atatcgtttg actgcagcgc cgcatcaaaa tcggtgatca cccggccaat
     5221 ctcttctcgc tcgtcaccca gcgcccgcgc ccacaactct tccagtttgg ccttaaaggt
     5281 gcggttgatg agcatatcgc gcggataaat tttcaatgcg gataaccgcg tccgactctc
     5341 ttcaatctgc tgcgcgctca atgttaccgg gctgtggtta atcactctgg acttaacgct
     5401 gccgtcttcc agaagcacgt caacttcaag caaaccgttg atatcataac taaagcgaat
     5461 atcaatcgac tgataagccc cggttctctt caacggcaca tcgaaggatt ccaccagaat
     5521 gttgttttta actttgtggt tttctccctg atagacgtta accgtaatag aatcctgttc
     5581 cgggtgcatg gttgaataag tttctacacg cgacacgggc acagtggtgt ttcgttcaat
     5641 aatcggcgag aaaatgccgg aaacgccctg gcggttaacc tcaacgccca acgagtaagg
     5701 gcaaatatca gtgaggatca cttcttcaat atcttcactg cgtaagcggc aggctgcctg
     5761 gattgctgcg cccagcgcga caatggtgct cggatcgtaa ctttgatacg gtaatttgcc
     5821 aaacagacgc acggcgattc gctgcaccag cggcatttgt gacgcgccgc caaccagcac
     5881 cagactgtcg atttgactcg gcttcagacg cgcatcgcgc aacgcctgtt caatcggcac
     5941 tcgcaagcga ttgagcagcg gcaaccacaa atcttccagt tcgttctcgt aaaattcgca
     6001 ttcccgcgtt tcatcctggt actgccagcg aatgtgtatt ggcgattgat tgctacattt
     6061 tgccgcttcc acacaggcat acaaggctgc cagttcactc tcgttaagcg tggtcctggc
     6121 gacatccgcg cgttttaaaa cctcatcgac cagcatatgg gtaaaatctt cgccaccgag
     6181 gaagttgtcg ccagcggagg cgtgaacttc aatcaccggc gtggcgtact caagcaccgt
     6241 gacgtcaaac gtgccgccac cgagatcaaa caccagcgaa cgggtatttt gttgggtatg
     6301 caggccatac gccatcgcag ctgctgtggg ttcattaatt aagcgtaccg catttaaacc
     6361 ggctaactcc gctgctaaac gggtatgctt gcgttgttca tcgctgaaat aagccggaac
     6421 agagatcacc acatctttaa tcggacgttg cagaaattct tcggcatctt cttttaatga
     6481 gcgtaatacc aaagaggaca gttctggcgc gttaaatgtg tcgctgccta accgccagtt
     6541 ggtattactg cccattgcac gtttaaataa cgctgccgtt ttatccggat gcgaagtacg
     6601 ccgtgataca gccggttttc cgactaaaat atgattattt tcatccatgc taattatgga
     6661 tggtgttaaa tattcaccga acttatttgg aattaattgc gcggcaccgt ctttccagac
     6721 ggcaattaaa ctattggtag taccgagatc gataccaatg gcgagttctg cattat
//
LOCUS       DAZFBR010000190         6640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFBR010000190 DAZFBR010000000
VERSION     DAZFBR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.190"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            269..394
                     /locus_tag="ACHJZ2_004671"
                     /pseudo
     CDS             269..394
                     /locus_tag="ACHJZ2_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            459..719
                     /locus_tag="ACHJZ2_004672"
                     /pseudo
     CDS             459..719
                     /locus_tag="ACHJZ2_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            769..1008
                     /locus_tag="ACHJZ2_004673"
                     /pseudo
     CDS             769..1008
                     /locus_tag="ACHJZ2_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1056..1673)
                     /gene="ureG"
                     /locus_tag="ACHJZ2_004674"
     CDS             complement(1056..1673)
                     /gene="ureG"
                     /locus_tag="ACHJZ2_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO4570636.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(1685..2359)
                     /locus_tag="ACHJZ2_004675"
     CDS             complement(1685..2359)
                     /locus_tag="ACHJZ2_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO4570637.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(2360..2824)
                     /gene="ureE"
                     /locus_tag="ACHJZ2_004676"
     CDS             complement(2360..2824)
                     /gene="ureE"
                     /locus_tag="ACHJZ2_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO4570638.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2834..4537)
                     /gene="ureC"
                     /locus_tag="ACHJZ2_004677"
     CDS             complement(2834..4537)
                     /gene="ureC"
                     /locus_tag="ACHJZ2_004677"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO4570639.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(4530..4850)
                     /locus_tag="ACHJZ2_004678"
     CDS             complement(4530..4850)
                     /locus_tag="ACHJZ2_004678"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO4570640.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4859..5161)
                     /locus_tag="ACHJZ2_004679"
     CDS             complement(4859..5161)
                     /locus_tag="ACHJZ2_004679"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO4570641.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(5252..5950)
                     /locus_tag="ACHJZ2_004680"
     CDS             complement(5252..5950)
                     /locus_tag="ACHJZ2_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO4570642.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            6331..6606
                     /locus_tag="ACHJZ2_004681"
     CDS             6331..6606
                     /locus_tag="ACHJZ2_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570643.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         1554 a         1705 c         1745 g         1636 t
ORIGIN      
        1 gcgagccgtt gcggaggctt gctctctcct ttaacctcat gtgcaatatc cgtaacgcgc
       61 tcacataaat catcatctga taatataaca acacccatta ttttattctg acctaaaaac
      121 ttcttatgaa ataaacgttt acccgacgta tctgatcccg ctgtttttac ctgtatcctt
      181 taacatactt gagaagcccg ctgaaaaaat ataccgtaaa ggcggtttct ctgtcttcca
      241 taaacctgtc ggataagaag ggcattaaat gaaaaccaaa aaagttatga tgattgcact
      301 ggtcagctct gcgctggctc tttcaggatg tggagccatg ggcacagcga tcaaaaaacg
      361 aaatctggaa gtaaaaaccc agatgagtga aacctgaggt agcctgagtt taacggacac
      421 tccttcctga aatagaatgg catcagaagg agctaataat gagcagaaaa acccaacgtt
      481 actctaaaga gttcaaagcc gaagctgtca gaacggttct tgaaaatcaa ctttcgatca
      541 gtgaaggcgc ttcccgatta tctcttcctg aaggcacttt aggacaatgg gttaccgccg
      601 ccagaaaagg gctcggtact cctggttccc gcacggtggc tgaactggaa tctgaaattc
      661 tgcaactgcg taaggcgtta aatgaagctc gccttgagcg agatatatta aaaaaagcag
      721 ctgtagattc aatctgtcaa tgcaacaccc ctttcaatta tctctttcac tgcccgcgac
      781 ggtatcggtt atcagtttga cctgctggct aacggtgaaa ccgtactgcg tgatgcatcc
      841 ggcgaaaaac gcatcaaggg tgaaaaagac ggtcgtatgc tcatgaatgt tggcctgaat
      901 gcggaaattc gcgacaacat ccgcttcggt ctggagtttg aaaaatcggc atctggtaag
      961 tacaacgtgg ataacgcggt gaacgccaac ttccgttatt ccttctgata aaaacaaggg
     1021 gccgcctgcc ggccctgata cagaggatgt tgtgattact tacccagcat gccctgctct
     1081 tcgataaagg cgatgatgtt ttgcagacct tcaccacttt tcaggttagt gaacccccat
     1141 gggcgttctc cgcgcatacg taaagtatca cgctccatta cctcgaggga ggcgcccacg
     1201 tatggtgcaa ggtcagtttt attgatcacg aggaagtcgg atttggtgat ccccggaccg
     1261 cctttacgcg ggatcttttc tccttctgcc acatctatga catatatggt aaggtccgcc
     1321 agttcggggc tgaaggtcgc actcaggttg tcgccgccgc tttccacaaa gatcagctcg
     1381 agattaccaa atttctcact cagtgcttcc acggctgcca gattcattga tgcgtcttca
     1441 cgaatggctg tatgagggca gccccccgtt tcgacaccga caatgcgttc cgcttccagt
     1501 gctccggcct cagtgagaat gcgttgatct tctctggtgt agatgtcgtt ggtgactacg
     1561 gctaattgcc aggtgtcacg catggatttg cagagggctt ccagaagcgc ggttttacct
     1621 gagccgaccg gaccgccgac gccgacacgc agcggttgct tgattatgtt cattttattt
     1681 tctcctaaga acggaataat cgggaatact gggtttcatg tcgcgcggaa gcgatagatg
     1741 caagtggcgt tgctgaacca attgcgtgat ccggggttgc aagggcactg tccatgttcg
     1801 ccgcatagcg ggcacacagg cgcataatta actgctgggc agccagctgg ccgtagggta
     1861 ccagcctgac gcccgccatc actgcgcttt caatccagct gtagccaagg ctcagggcca
     1921 ggtctgaaac ggatatttgc cagcgtacgc cgagccatgc cataccggtt aactggcttt
     1981 gctggcatag tttgcgccat tcggccgggc agtcgggctg ccagtcgacc agaagtctgg
     2041 tgaaggctgc accgcggtta cgttcttcat cgcgtagctc acgggtttcc ctgcaggcca
     2101 gcagataagc ggtccagcgc ccggcactgt caggatcgcc cgcttcacag gcccggtata
     2161 gccgggcaag cagtggcagg tcgacggcaa aaaaactctg ttccatctgc aactgctgcc
     2221 agtgttcaaa ggcggcgcta tcttctaccc agcctgcttc cactgcccac tccagtccct
     2281 gggaccagct gtagcctcca acagggaggc tgttactggc aagctgcatc agccgcagac
     2341 gtttttccgg agtcggcatt cagtggctgt gctcctgatc gtgattatgg gcgtgcgact
     2401 tgctggcgta agcgccggct tccggctcaa acggaagatg tgcaaaatca acatccagcc
     2461 caaactggcg cagcatatca tcgaggacgt gatcgtgatg gtagcgcagc tctccgggca
     2521 ttatttgcag cggaacgtgg cgattgccga gatgccagca ggctttcgcg agcataaaag
     2581 gatctgcgca gcgcactaca gaaacggctt catcagcggc gatcacctta atgaattcat
     2641 caccgttttc gttactgaga atatcaccat cgcgcagcag cagaccgcgt ggcagcaaca
     2701 gtccagcctg acgtccatcg ttaagggtga ctttaatacg gctttttacc cgcatgtcaa
     2761 cagggagggt aacgctggca gttgtctgag ccggggtctc cacccgccgg gtcaaataaa
     2821 gcatttgtgc tccttaaaac aggaaataac gttgcgccat tggcagaatg tccgccggtt
     2881 cactggttat cagttcgccg ttgatacgca cttcgtaggt ttgtgaatcc acggttatat
     2941 caggcaacag gctgttgtgg cgcatatcgg ctttttgtac tgtgcggcag ccttttacca
     3001 cagcagttgt gctgtgcagg ttaagctgtt cagcgacgcc atttgctgct gctgcctgcg
     3061 acaggaaagt cacacgacag cggtgacggg cactgcccaa tgcagcgaac attgggcgat
     3121 agtgcaccgg ctgcggtgtg gggatagagc cgttgatatc acccatcggc gccatggcta
     3181 tcattccgcc tttgacgata gtcgccggtt ttacgccaaa gaacgccggg gaccagagca
     3241 ccagatccgc cagttttccc acttcaatcg agccgacttc atgagcaata ccgtgggtta
     3301 atgccggatt aatggtgtat ttagcgatat agcgcttcac gcggacgttg tcgttatcac
     3361 cactttcttc cggtaacggg ccgcgctgaa ctttcatccg gtgtgccacc tgccaggttc
     3421 gtaacactac ttctccgacg cgtcccatgg cctgcgaatc ggacgaggtg agggagaacg
     3481 cgccaaggtc gtgcaggacg tcttccgcgg caatggtttc ctggcgaatg cgcgattcgg
     3541 caaaggctac gtcctcggcg atatccgggt caagatgatg gcaaaccatc agcatgtcca
     3601 gatgctcatc aatagtgttg acggtatagg gtagcgtcgg attggttgag gaaggcagaa
     3661 tattggggtg cgcgcaggcg gtgataatat ccggagcatg accaccacct gcaccttctg
     3721 tatggaaggt atggatagtg cgcccgccga tggcagtcag agtatcctca acgaatcctg
     3781 attcgttaag cgtatcactg tgtagcgcaa cctgaacgtc catttcgtcg gctacagtca
     3841 gtgcgcagtt gattaccgca ggtgtagctc cccagtcttc gtgaattttg aggccgataa
     3901 ccccggccgc gacctgctca cgaagcgcat ccggattgga gcaattgcct ttacccagca
     3961 acccgatatt gaccggcagg ctgtctgcag cctgcagcat ttgataaatg taccatggtc
     4021 ctggggtaca ggttgtggcg ttagaacccg ctgtcgggcc agtaccgcca ccgatcatgg
     4081 tggtaatgcc ggatgtcagc gcttcttcag cctgctgtgg gcagatccag tgaatatgcg
     4141 tatcgacgcc acctgcggta acgatcctgc cttctgcggc aataatttcc gtggatacgc
     4201 cgattgggat agtgacgttg ggttgtatat caggattacc ggctttgccg atagcaaata
     4261 tccttccatc tttgacgccg atatcggctt taacgatccc ccagtaatcg atgatcaggg
     4321 cattggtcag caccagatca gcgcagccgg cggagagcat ttgcccctgt cccataccgt
     4381 cgcggattac tttaccgccg ccgaatttga cctcttcgcc gtaggtagtt aaatcatctt
     4441 cgacctcgat ccacagctca gtgtctgcca gacgaatttt atcaccggtg gtagggccga
     4501 acatgtcagc ataggcctgg cgtgaaatat tactcatcat ttgcctccag ctcacccatt
     4561 atatctccgc ggaaaccgtg cacgatacgg gcaccgctca gcgccaccag ttcaacctca
     4621 cgcttttggc ctggttcgaa gcgcactgca gttccggcgg cgatattgag acgatagcct
     4681 cttgccttct ggcgatcgaa cttcagtgcc gggttaacct ccgcgaagtg ataatgggaa
     4741 ccgacctgaa ttggtcggtc tccgtgattt tcaacgataa tactgcaggt agcccggccg
     4801 atgttaagct ctatatagcc aggtttaacc ttatattctc cagggatcat ctgttacctc
     4861 agattatcgg attatggacg gtaaccagct tggagccgtc cggaaaggtg gcttcgacct
     4921 ggatatcggg gatcatttct ggtatgcctt ccatgacctg ctcgcgactc aggacatgcc
     4981 gtccttcttc catcagcgca gcgacgcttt tgccgtcgcg agcgccctcc attataaaag
     5041 cgctaatcag ggccacggat tcgggatagt taagtttcag gccgcgggcc agacgacgct
     5101 ctgccagcag tgcagcggta aacagtaata gtttgtcttt ttctcttgga gtcagttcca
     5161 tagcgttctc ttaagtcagc cagatccggg gaagtaccgg tgatttgtca gtcagttgcg
     5221 gccgcataaa ttgccagata tcacgcatta cctactggca acgcagatta tcatcactta
     5281 aaaagcgaac tgtcagtaga ccatcggtga gcgtggctcc ggcgtagttt tccagtggtg
     5341 tgagtttttc gcgtatgtct tccagttgcg tttcattccc caggtagaac agcatcgttc
     5401 ccacccatgg tttccgggca acgcaggtaa gttctccatt ggccacgtgc agacgctcaa
     5461 tcaacagcgg agaaccgtcg acccatatct ccagacggtt agcgagtgtt ccgtggctga
     5521 aggtttctcc gatgaccgga cgtccaaggc acagaagatc ccaggccagc agtgtactgg
     5581 aggacgacag atgaaacacg gtatgcaacg tggcgtgagc gccgggaaaa aaaatcgaat
     5641 cctgtggcaa ccactccagc gttgcgttat tgcacagcgt caggttttgc tgaaggtgcg
     5701 cctgcgcgcc gctgctgcgg taaaatttac tggcaccagg catggtgatc agcgtatggc
     5761 aatcagagtc aattattgcg ttgatgatga gttcgtcacc gccgacgata ccaccgggtg
     5821 gatgaagcag acagaggtgg caggtttctt cttcgggata aaaaggacgc tgaacagtga
     5881 gcagaccgac atgatgacgg gtggtgagcg tggttttccc gtgcagacgc tgaaaacgga
     5941 gatccagcat ggcctgccag ccttttttat tttgtggtaa cgcggtactg agcaatctta
     6001 cgcccctgat aaataaagat aaaacgacag taaagagccg gatttccttg ttatataaaa
     6061 gatgatgcct ttccagtgag ttttcgtctg aataatacga atgtttgttt tcgtcatgaa
     6121 gcgttccggc tcccggggct atacatcgcc agaaaagcaa tgcatattgt cgggattatc
     6181 cagagaatca ggcagaaaga tccgacaatg ggctgtgatt atgccatctg gctcagaatg
     6241 atatcaaggt gttatgggga taacttgctg atacaatagc agtatgacag attcattaat
     6301 gcggaaactg ctcactgaaa cagagtgttc atgactgagc actggtcggg tcatgcagat
     6361 gataatttcc ccttttcttg gggaagcgac agtattttca tgcatgcctg tcttcagtca
     6421 tacggggaga gcatctgtga tacagtccat acatttgttg cggacgtcgc tcctgagtca
     6481 ctgactggtc tgcaaacagt ttatctgtac acggcagggc ttctgtgtga tatcgttttt
     6541 catatcgagg gggggcctcc agagatgaat ggaataataa tatttcctga tgatataata
     6601 ccttagagaa tttctgtgta tgctattgtg aacatatttt
//
LOCUS       DAZFBR010000191         6494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFBR010000191 DAZFBR010000000
VERSION     DAZFBR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.191"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(169..2202)
                     /gene="betT"
                     /locus_tag="ACHJZ2_004682"
     CDS             complement(169..2202)
                     /gene="betT"
                     /locus_tag="ACHJZ2_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO4570644.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2331..2918
                     /gene="betI"
                     /locus_tag="ACHJZ2_004683"
     CDS             2331..2918
                     /gene="betI"
                     /locus_tag="ACHJZ2_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO4570645.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2932..4404
                     /gene="betB"
                     /locus_tag="ACHJZ2_004684"
     CDS             2932..4404
                     /gene="betB"
                     /locus_tag="ACHJZ2_004684"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO4570646.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4418..6106
                     /gene="betA"
                     /locus_tag="ACHJZ2_004685"
     CDS             4418..6106
                     /gene="betA"
                     /locus_tag="ACHJZ2_004685"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO4570647.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPTILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
BASE COUNT         1605 a         1789 c         1768 g         1332 t
ORIGIN      
        1 taatacaaaa taaaatcgtt aattaataaa atataaaatc atgaagtgat acaagaggaa
       61 tttcaggata tgacatgaca atcatatata atatatgcat aattttttac agcgagtcaa
      121 tgattcccgc gccatatgca gacatggcgc ggttttatgc aataacaatc acgcgtccgg
      181 gaacatcacg ctatggcccg gcgcttcacg atggagatga ataaagttaa ggtgccgctc
      241 gtactggtca agaatatcgg tgatcacctg ctctttgctg tagtccatca ggtcgttgcc
      301 ctggctgcct tctaacagga aggtttccag ccggtagtag gtcgatttcc cgctacgtgc
      361 gcggtaggta aagcccggca ccgagtattg ctgcggccaa atctgataga caaagttttg
      421 ctcttcgccc atatgcacca acaaatccag atgccccaac tgttgtccct cttccggcgg
      481 caggcttttt agctccacgt acgcgccgcg caaccgcaac tcctgcgcca cttcttccat
      541 tgccgggtaa cagaccgtct ccatcatctg tttagtgtaa cgcgtgcccg gataattcat
      601 caggcgcgag agacgttttt tccagctcag gcgatcctga agccccagcg gtcgcggtgc
      661 ggtatcgcgg ttggcacttt cacggcggta atcttctacc ttcagagatt tatacaaccc
      721 cgccatcacg aagaagatca caaagctgaa cggcagcccc ataatcaccg tggtgttttg
      781 cagcgcggat atcccgttgg tcatcagcat gccgagcgtc agcaggccaa tcgccaccga
      841 ccagaagacg cgcagccagc cgggggcgtc gctgttgata tctttaagct gcgaggtgaa
      901 attccccagc accagcgccc cggagtccgc cgaggtcaca taaaacagca ggccagtaat
      961 ggtggcgacg gaggcgctaa aggtaaacgc cggatactgc gccagcaggc tgtagaagcc
     1021 gcgctccgga tggaccatcg cttcctcggc aaatgccgcg ccgccgtgga tgatttcata
     1081 cagcgcgcta ttgccgaaca ccgagagcca taacagcgtg aaggtaaacg gaataatcaa
     1141 cgtgcccagc acgaactggc gaatggtacg cccacgcgag atacgcgcca ggaacaagcc
     1201 gacaaacggc gaccatgcca cccaccatgc ccagaagaag agcgtccagt tattcatcca
     1261 ctcaaccggg cggtcgaagg caaaactgtt gagcgtcatg cccataaagc gattcacata
     1321 gtcgccaaca ttcagcacca gtgcattaag caggaacgaa gtgtcgccca taaacaacac
     1381 gaacaggatc aatcccagcg ccagcgcgac attaagctcc gataacacgc gaatgccctt
     1441 atcgacaccg gatgtcaccg agatagtggc gattatcacc gacaaggcga tcagtgccgc
     1501 ttttgccgcc atcgaatcgg gaatatcaaa cagtacgctc aagccatagt taagctgcac
     1561 cacaccgata ccgagcgtag tggcaatacc gaagatagtg ccgatcaccg ctgcaatatc
     1621 cactgagtga cctatcggcc cgttaatccg tttaccgaag atcgggtaca gcgccgagcg
     1681 gatggtgagc ggcaaattat aacgatagct aaagtatccg agcgccatgc ccatcagcgc
     1741 atacatcgac cagccggtta agccgtagtg aaacagcgtc cagaccatcg cctgacgcgc
     1801 ggcctcaatc gtctgtcccg cgccttccgg cggctgcata tactgcgtta ccggttcggc
     1861 tacggaaaag aacatcagat cgataccgat cccggcagca aacagcatcg ccgcccaact
     1921 cagcaggctg aattccggtt tggattgttc tggcccgagc ttcaccgaac caaaacgcga
     1981 acaagcgata cagaccacaa agacaatata gagcgttgcc gccagcagat agtaccaacc
     2041 gaaggtttta gaaacccagt ccagcgtgcg gccaatccac agggccgaga agtcgcgaaa
     2101 caggatcgtt gtcagggaaa acaacaaaat cagtccggcg gaggtgtaaa acaccaccgg
     2161 attgattttg tccttttccc tgctgtgtga aaggtctgtc atccagtatc cccactgtta
     2221 ttgttaccat ttaaaatcaa attcgtaaca attaagacac attttatatt gaacgtccaa
     2281 tcaataaccg ctttaataga taaacaacgc tgatgaatgg agtggcaaaa atgcccaaat
     2341 tggggatgca gtcgatccgg cgcagacaac tgatcgacgc cacactggaa gcaataaatg
     2401 aagtgggcat gcacgatgca acgatcgcgc agatcgcccg ccgtgcaggc gtttctacgg
     2461 ggatcatcag ccactatttc agggacaaaa atggtctgct ggaagcaacc atgcgcgata
     2521 tcaccagtca gctgcgtgac gcggttttga atcgattaca tgcacttccg cagggcagtg
     2581 cagagcagcg attacaggcg attgttggcg gaaacttcga tgaaacgcag gtgagcagtg
     2641 cggcgatgaa agcctggctg gcgttctggg ccagcagtat gcatcagccg atgctctatc
     2701 gtttacagca ggtcagcagc cgccgcttgc tgtcgaatct ggtgagcgag tttcgccgcg
     2761 aattgccgcg ccaccaggca caggaagcgg gctacggcct tgccgcgctg attgacggat
     2821 tatggctgcg cgcggcactg agcggcaaac cgctggataa acctctcgca cactcgctga
     2881 cccgtcactt tattactcag catttaccca ccgattaacc gaggagacgt gatgtcccga
     2941 atggcagaac agcagcttta tatacatggt ggttatacct ccgccaccag cggtcgcacc
     3001 ttcgagacca ttaacccggc caacggtaac gtgctggcga ccgtgcaggc cgccgggcgc
     3061 gaggatgtcg atcgcgccgt gaagagtgcc caacaggggc aaaaaatctg ggcggcgatg
     3121 accgctatgg aacgctcgcg tattctgcgt cgggccgtcg atattctgcg cgagcgcaat
     3181 gatgaactcg ccaaactgga aaccctcgac accggaaaag cgtattcgga aacctcaacc
     3241 gtcgatatcg ttaccggtgc ggacgtgctg gagtactacg ccgggctgat cccgtcgctg
     3301 gaaggcagcc agatcccgtt gcgtgagaca tcatttgtct acactcgccg cgaaccgctg
     3361 ggcgtggtgg cagggattgg cgcatggaac tacccgatcc agattgccct gtggaaatcc
     3421 gccccggcgc tggcggcggg taatgcgatg attttcaaac cgagcgaagt cactccgctt
     3481 accgcgttaa agctggctga aatttacagc gaagcgggcc tgccggacgg tgtatttaac
     3541 gtgttgccgg gcgtgggcgc ggagaccggg caatatctga ccgagcatcc gggcattgcc
     3601 aaagtgtcgt ttaccggcgg cgtcgccagc ggcaaaaaag tgatggctaa ctcggcagcc
     3661 tcttccctga aagaagtgac catggaactg ggcggtaaat caccgctgat cgttttcgac
     3721 gatgcggatc tcgatctcgc cgccgatatc gccatgatgg caaacttctt cagctccggt
     3781 caggtgtgta ccaatggcac ccgcgtcttc gttccggcga aatgcaacgc cgcatttgag
     3841 cagaaaattc tggcgcgcgt tgagcgcatt cgcgcgggcg acgttttcga tccgcaaact
     3901 aacttcggcc cgctggtcag cttcccgcat cgcgataacg tgctgcgcta tatcgccaaa
     3961 ggcaaagagg aaggcgcgcg cgtactgtgc ggcggcgatg tactgaaagg cgatggcctc
     4021 gataacggcg catgggttgc accgaccgtg ttcaccgatt gcagcgacga gatgaccatc
     4081 gtgcgtgaag agatcttcgg gccggtgatg tccattctga cctacgagtc ggaagacgaa
     4141 gtcattcgcc gcgccaatga taccgactac ggcctggcgg cgggtatcgt gacggcggac
     4201 ctgaaccgcg cgcatcgcgt cattcatcag ctggaagcgg gtatttgctg gatcaacacc
     4261 tggggcgaat ccccggcaga gatgcccgtt ggcggctaca aacactccgg tattggccgc
     4321 gagaacggcg tgatgacgct ccagagttac acccaggtga agtccatcca ggttgagatg
     4381 gctaaattcc agtccatatt ctaaccagga ggtttatttg caatttgact acatcattat
     4441 tggtgccggc tcagccggca acgttctcgc tacccgtctg actgaagatc cgaatacctc
     4501 cgtgctgctg cttgaagcgg gcggcccgga ctatcgcttt gacttccgca cccagatgcc
     4561 cgctgccctg gcattcccgc tacagggtaa acgctacaac tgggcttacg agacggaacc
     4621 tgaaccgttt atgaataacc gtcgcatgga gtgcggacgc ggtaaaggcc tgggtggatc
     4681 gtcgctgatc aacggcatgt gctatatccg tggcaacgcg atggatctcg acaactgggc
     4741 aaaagaaccc ggtctggaga actggagtta tctcgattgc ctgccctact accgcaaggc
     4801 cgagacccgc gacgtgggcg agaacgacta ccacggcggc gacggcccgg tgagcgtcac
     4861 cacctccaaa cccggcgtca atccgctgtt tgaagcgatg attgaagcgg gcgtgcaggc
     4921 gggctacccg cgcacggacg atctcaacgg ttatcagcag gaaggtttcg gcccgatgga
     4981 tcgcaccgtc acgccgcagg gccgccgcgc cagcaccgcg cgcggttatc tcgatcaggc
     5041 caaatcgcgc ccaaacctaa ccattcgtac tcacgccatg accgatcaca ttatttttga
     5101 ctgtaaacgc gcggtgggcg tcgagtggct ggaaggcgac agcaccattc cgacccgcgc
     5161 gacggcgaac aaagaagtgc tgttatgtgc aggcgcgatt gcctcaccgc agatcctgca
     5221 acgctctggc gtcggcaacg ctgaactgtt ggccgagttt gatattccgc tggtgcatga
     5281 tttacccggc gtcggtgaaa atcttcagga tcatctggag atgtatctgc aatatgagtg
     5341 caaagaaccg gtttccctct accctgccct gcagtggtgg aatcagccga aaatcggtgc
     5401 ggagtggctg tttggcggca ccggcgttgg tgccagcaac cactttgaag caggcggatt
     5461 tattcgcagc cgagaggaat ttgcgtggcc gaatattcag tatcacttcc tgccggtagc
     5521 gattaactat aacggctcga atgcagtgaa agagcacggc ttccagtgcc acgtcggctc
     5581 gatgcgctcg ccaagccgtg ggcatgtgcg gattaaatcc cgcgacccgc accagcatcc
     5641 gacaattctg tttaactaca tgtcgcacga acaggactgg caggaattcc gcgacgcaat
     5701 tcgcatcacc cgcgagatca tgcatcaacc cgcgctggat cagtatcgtg gccgcgaaat
     5761 cagccccggc acggaatgtc agacggatga gcagctcgat gagtttgtgc gtaaccacgc
     5821 cgaaaccgcc ttccatccgt gcggtacctg caaaatgggc tacgacgaga tgtccgtggt
     5881 tgacggcgaa ggccgcgtgc atgggctgga aggcctgcgc gtggtggatg cgtcaattat
     5941 gccgcagatt atcaccggga atttgaacgc cacgacgatt atgattggcg agaaaatggc
     6001 ggatatgatt cgcgggaagg aagcgttgcc gaggagcacg gcgggatatt ttgtggcaaa
     6061 tgggatgcca gtaagagcga aaaaaatgag tcgtgatttg aactgactca ggaaccgatc
     6121 agattgagag ttaccgttcc aggtatgggg acagaatcgt tatataaaac ataggggtaa
     6181 ctctcatgct tcatagtgac aacctcgcca gcaaattcta agctgactcg ttcaatctcg
     6241 ctgatgaagg tactatgaat gcactgataa accgcaggtg aaatgccact cagcttaaga
     6301 attacgccgc tagtgttcct ggctatggtg acttgttcac tcccgctcac ggttagcata
     6361 tacgaaaagg gttcttgtaa cgaggacaaa aataagtcag accaaaacaa cgtggagctt
     6421 taaacacaat tcgtataatt tattagcagt gatttatttt tactcttctt taattaatca
     6481 tcatgcttaa atat
//
LOCUS       DAZFBR010000192         6390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFBR010000192 DAZFBR010000000
VERSION     DAZFBR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.192"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..171)
                     /locus_tag="ACHJZ2_004686"
     CDS             complement(<1..171)
                     /locus_tag="ACHJZ2_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570648.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINE"
     gene            500..1372
                     /locus_tag="ACHJZ2_004687"
     CDS             500..1372
                     /locus_tag="ACHJZ2_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4570649.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVWGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1744..4593
                     /gene="cah"
                     /locus_tag="ACHJZ2_004688"
     CDS             1744..4593
                     /gene="cah"
                     /locus_tag="ACHJZ2_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO4570650.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            4704..>6390
                     /locus_tag="ACHJZ2_004689"
     CDS             4704..>6390
                     /locus_tag="ACHJZ2_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO4570651.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR"
BASE COUNT         1610 a         1613 c         1788 g         1379 t
ORIGIN      
        1 ttcatttatt tttaatgcat aatcagatgc cgcctcatca ttatcctcag tattatcata
       61 gccgaaaact tctcccggac acgggcgttc acgataatag tcatcgtcat agttttcatc
      121 gtcagataat gatatgtcgt attctgaata tgattcacca caattataca tgaaatcatc
      181 tcctttgggg ggggcaataa ttagtgagtt tattcgaaaa aatgatgagc gccagaccaa
      241 tccacccaac ttttgattat aatcatgaag atacttgtct cagtacaaat tgaatcaaac
      301 tcaatgcagc atgattcacc tttatattct ctcagggaaa ccaaatacca ctactcaact
      361 attaagaaca aaattcggga tgtatgatta tcagaagata cctgcatcct aaataatttc
      421 agacagatat tatcgttgtg aatcccccgc cacctttccg gtgcgcgggg ttttgtcttt
      481 tttcaccggg aatacatgta tgaatccgtc tgatgccatt gaggcaattg aaaaaccgct
      541 ctcctccctg ccttactcgc tttcccgtca catcctggaa catctgcgca aactcaccag
      601 tcacgaaccc gtgattggca ttatgggtaa aagcggggcc ggtaaatcct cactctgtaa
      661 tgcactgttt cagggggagg tcaccccggt cagtgatgtt cacgccggca cccgggaagt
      721 gcggcgcttc cgtctgagtg gccatggtca cagcatggtt atcactgacc tgcccggggt
      781 gggcgagagc cgggacaggg atgcagagta tgaagccctg taccgtgaca ttctgcctga
      841 actggacttg gtactgtggc tgattaaagc cgatgaccgt gccctgtctg tggatgagta
      901 tttctggcga cacatactgc accggggaca tcagcaggtg ctgtttgtgg tgacgcaggc
      961 cgacaaaaca gagccctgcc atgaatggga tatggcaggt attcagcctt ctcccgcaca
     1021 ggaacagaac attcgcgaaa aaacagatgc ggtattccgg ctgttccggc ccgtacatcc
     1081 ggttgttgcc gtatcggccc gcaccggctg ggaactggat acgctggtca gtgcgctcat
     1141 gacagcgctt cccgaccatg ccgccagtcc cctgatgacc cgcctgcagg acgagctgtg
     1201 cacggaatct gtctgggggc aggcccgtga acagtttacc ggtgcggtgg accggatatt
     1261 tgacacggtg gagagcgtct gtgttgcctc tgttgcacgc acagttctgc gtgccgtccg
     1321 tgacacggtg gtctctgttg cccgcgcggt atggaactgg attttcttct gaacagtctg
     1381 ccatgggaaa tgtctgatta cattgcgtta accaactgaa aacatgatac aaacacctca
     1441 tgcccctcct gtttctcttg cgtgactgct ctactgttaa tagaataaaa cgatcgataa
     1501 aacaggtatc gatcgtttat atcgatcgtt tatagtggtt aatggcctgc gaaaaggcag
     1561 ctgaatctct tcatcatgca gaacggaatt gcacacaaca gactgatact tctctgtctg
     1621 atgaaggggg agagcgttta cgcccgggac gtatcgctgt gcccgataac tctgttttgt
     1681 acctgccggt atccactttt gtgggtaccg gcttttttat tcaccctctg taaggaaaag
     1741 ctgatgaaac gacatctgaa caccagctac aggctggtat ggaatcacat tacgggcacc
     1801 ctggtggtgg cctccgaact ggcccgctca cggggaaaac gcgccggtgt ggcggttgcg
     1861 ctgtctcttg ctgctgtcac atcagtcccg gcactggctg ctgacaaggt tgtacaggcg
     1921 ggagaaaccg tgaacgatgg aacactgaca aatcatgaca accagattgt cttcggtacg
     1981 gccaacggaa tgaccatcag taccgggctg gaactggggc cggacagtga agaaaacacc
     2041 ggtgggcaat ggatacagaa tggcgggata gccggaaaca ccactgtcac cacaaatggt
     2101 cgtcaggtcg tgctggaggg gggaacagcc agtgatacgg ttattcgtga cggcggggga
     2161 cagagcctga acggactggc ggtgaacacc acactgaata acagaggcga gcagtgggtg
     2221 catgagggcg gggttgccac cggtacaatt atcaaccgcg acggttacca gagcgttaaa
     2281 agtggcgggc tggcaacagg aaccatcatc aacaccggcg cagaaggcgg ccctgattct
     2341 gacaactcgt atacgggtca gaaggtccag ggaacagcag aatccaccac catcaacaaa
     2401 aatggacggc agattatctt attttccggg ctagcccgtg acactctcat ttacgcaggt
     2461 ggtgaccagt cggtacacgg aagggccctg aataccacac tgaatggcgg ttaccaatat
     2521 gtgcacaggg acggacttgc gctgaacacg gtaattaacg aggggggctg gcaggttgtt
     2581 aaggcaggtg gcgctgccgg taacaccacc ataaatcaga acggtgaact gagggtacat
     2641 gccggcgggg aagccactgc agtcacccag aacacgggcg gtgcactggt taccagtact
     2701 gctgcaactg tcatcggcac aaaccgtctg gggaatttca cggtggaaaa cggtaaggct
     2761 gacggtgttg ttctggaatc cggcggtcgt ctggatgtac tggagagcca ttcagcacag
     2821 aataccctag tggatgacgg cggtaccctg gcagtgtctg ccggcggtaa ggcgacaagt
     2881 gtcaccataa catccggtgg tgccctgatt gcagacagtg gtgccactgt tgaggggacc
     2941 aatgccagcg gtaagttcag tattgatggc acatccggtc aggccagcgg cctgctgctg
     3001 gaaaatggcg gcagctttac ggttaatgcc gggggacagg ctggcaacac cactgtcgga
     3061 catcgtggaa cactgacgct ggctgccggg ggaagtctga gtggcagaac acagctcagt
     3121 aaaggcgcca gtatggtact gaatggtgat gtggtcagta ccggcgatat tgttaacgca
     3181 ggggagattc gctttgataa tcagacgaca ccgaatgccg cgctgagccg tgctgttgca
     3241 aaaagtaact ccccggtaac gttccataaa ctgaccacca cgaacctcac cggccagggc
     3301 ggcaccatca atatgcgtgt tcgccttgat ggcagcaatg cctctgacca gctggtgatt
     3361 aatggtggtc aggcaaccgg caaaacctgg cttgcgttta caaatgtcgg aaacagcaac
     3421 ctcggggtgg caaccaccgg acagggtatc cgggttgtgg atgcacagaa tggcgccacc
     3481 acagaagaag gtgcgtttgc cctgagtcgc ccgcttcagg ccggcgcctt taactacacc
     3541 ctgaaccgtg acagcgatga agactggtac ctgcgcagtg aaaatgctta tcgtgctgaa
     3601 gtccccctgt atacatccat gttgacacag gcaatggact atgaccggat tctggcaggc
     3661 tcccgcagcc atcagaccgg tgtaaacggt gaaaataaca gcgtccgtct cagcattcag
     3721 ggcggtcatc tcggtcacga taacaacggc ggtattgccc gtggagccac gccggaaagc
     3781 agcggcagct atggcttcgt ccgtctggag ggtgacctgc tcagaacaga ggttgccggt
     3841 atgtctctga cgacaggggt gtatggtgct gcaggccatt cttccgttga tgttaaggat
     3901 gatgacggtt cccgcgccgg cacggtccgg gatgatgccg gcagtctggg cggatacctg
     3961 aatctggtac acacatcctc cggcctgtgg gctgacattg tggcccaggg aacccgtcac
     4021 agcatgaaag cgtcatcgga caataacgac ttccgcgccc ggggctgggg ctggctgggc
     4081 tcactggaaa ccggtctgcc cttcagtatc actgacaatc tgatgctgga gccacaactg
     4141 cagtacacct ggcagggact ctccctggat gacggccagg ataacgccgg ttatgtgaag
     4201 ttcgggcatg gcagtgcaca acatgtgcgt gccggtttcc gtctgggcag ccacaacgat
     4261 atgacctttg gtgaaggcac ctcatcccgt gacaccctgc gcgacagtgc aaaacacagt
     4321 gtgagtgaac tgccggtgaa ctggtgggta cagccttctg ttatccgcac cttcagctcc
     4381 cggggtgaca tgagcatggg gacagccgca gccggcagta acatgacgtt ctcaccgtcc
     4441 cggaatggca cgtcactgga cctgcaggcc ggactggaag cccgtatccg ggaaaatatc
     4501 accctgggcg ttcaggccgg ttatgcccac agcgtcagcg gcagcagcgc tgaaggctat
     4561 aacggtcagg ctacgctgaa tatgactttc tgattatacg gtatcccgga aatacgattt
     4621 caggaaaatt catgcagtaa tgggaaagga cgctcattgc tgttcaccgc agttctgaat
     4681 tattgaattt ataaaggtga atgatgcacg aaaagaacat cgccctgctt tgtgatgaag
     4741 ccgaccggct tttgcaactg aatatcaatc tgctccggca aatggttgat gagccggatg
     4801 tgttatctga cagtaagaac gaaaacgggc tgctttttga taaacggaaa gcgctgaaaa
     4861 gaattgagga gctggagggc gaacaaatca aaaccgcccg cagggagatg gtgctggctg
     4921 tggtcggcac gatgaaggcg ggcaaatcaa ccaccatcaa cgccattgtg gggcaggaaa
     4981 ttctgcccaa ccgtaaccgc cccatgacct cggtaccgac attgatccgc cacgttcccg
     5041 gaaaaacgga accggtcctc catctggaac atattcagcc cgtccgcaat ttattaatca
     5101 cactgcagga aaaactcgcc accccggcag ggcagcaagt cgcacagtcc ctgcagcaaa
     5161 ccggggatac ccgcgaacta ctggatattc tggcggatga tgtctggctt aaaaatgaat
     5221 accacgggga agacgaaatc tttaccggac tggcatcgtt aaatgacctg gttcgtcttg
     5281 ctgcggcaat ggggactgaa tttccttttg acgaatacgc agaagtgcag aaactaccgg
     5341 tgatcgacgt ggcgttcagc cacctggtgg gaatggatgc atgtcaggga acactcacac
     5401 tgctggatac ccccggccct aatgaagccg gacaaccaca gatggaaatg atgatgcggg
     5461 atcaactaca gaaagcctct gcggtgctgg cggtgatgga ttacacccag atgaactcga
     5521 aagccgatga ggaggttcgt aaagagctta atgccattgc tgacgtctca gccggtcgtc
     5581 tgtttgtgct ggtcaataaa ttcgatgaaa aagatcgcaa tggtgatggt gcggatgccg
     5641 tacgccagaa ggtgccagca atgctgaaca gcgatgtgct gcccgcctcc cgcgtttatc
     5701 ccggctcatc acgtcaggcg tacctggcta accgtgcgct tcatgagtta cgaaaaaacg
     5761 gtgcgcttcc ggttgatgaa gcctgggtcg atgattttat cagggaagct ttcggaccta
     5821 tggtggaaga ggacgactgg aaagacagca ctaaggtaaa taaaaaggca gaaaaactct
     5881 ggaatatatc tctgatcgat caactgataa cggaagtcat acagagctcc cattccagag
     5941 cggcggcact ggcggttgac tctgccgccg caaaactgat gcagaatgca gaaaatgtca
     6001 gcgaatatct gtcattacgt catcaggggc tgcagcaaag cattcagtca ctacaggcgc
     6061 atattaccag cctgctggcg gatatccagg agattgagga atgtcagaat caggtgaccg
     6121 gtgatgtcag aatggccatg gaggacatca ataccaaaac cggggaatta ctgacgaagg
     6181 tctgtgcctc actggaggag gagctgaacg actatttcag aagcggtaaa cgcaaagaac
     6241 agcaaatgct ggaggaagaa aattcagcgc agccccggga gagaaacgca ttcgctttct
     6301 ttcacgatat tttcggaacc ggaaatcagc atgacaggat gcgggacttt gatccggaca
     6361 gccccgaaat aaaattcagt gatcgcaggg
//
LOCUS       DAZFBR010000193         6178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFBR010000193 DAZFBR010000000
VERSION     DAZFBR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.193"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            268..1050
                     /locus_tag="ACHJZ2_004690"
                     /pseudo
     CDS             268..1050
                     /locus_tag="ACHJZ2_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1170..1357
                     /locus_tag="ACHJZ2_004691"
                     /pseudo
     CDS             1170..1357
                     /locus_tag="ACHJZ2_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1555..2406)
                     /locus_tag="ACHJZ2_004692"
     CDS             complement(1555..2406)
                     /locus_tag="ACHJZ2_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO4570652.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(2480..2722)
                     /locus_tag="ACHJZ2_004693"
                     /pseudo
     CDS             complement(2480..2722)
                     /locus_tag="ACHJZ2_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            complement(2947..4050)
                     /gene="zorE"
                     /locus_tag="ACHJZ2_004694"
     CDS             complement(2947..4050)
                     /gene="zorE"
                     /locus_tag="ACHJZ2_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorE"
                     /protein_id="HFO4570653.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            complement(4054..4761)
                     /gene="zorB2"
                     /locus_tag="ACHJZ2_004695"
     CDS             complement(4054..4761)
                     /gene="zorB2"
                     /locus_tag="ACHJZ2_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HFO4570654.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            complement(4768..>6178)
                     /gene="zorA2"
                     /locus_tag="ACHJZ2_004696"
     CDS             complement(4768..>6178)
                     /gene="zorA2"
                     /locus_tag="ACHJZ2_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HFO4570655.1"
                     /translation="PVLLGGLKTAFITSIVGMFFAILFNGMDAFFFANKRSALAENNP
                     ESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLIAQIKLLRTEISDSSQAQLANHTH
                     FSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDFNQNLTEQFGENFKALDASVKKLV
                     EWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIWEECKEIPLAMSELREVLQVNQHQ
                     ISELSRHLETFVAIRDKATTVLPEIQNKMAEVGELLKSGAANVSASLEQTSQQILLNA
                     DSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNSSETLTSTLGETITEMKQSGEEFL
                     KSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQLSSNADNMFDSIQTSFDKASAGL
                     TSQVRESIEKFALSINEQLHAFEQATEREMNREMQSLGNALLSISKGFVGNYEKLIKD
                     YQIVMGQLQALISANKHRG"
BASE COUNT         1685 a         1307 c         1252 g         1934 t
ORIGIN      
        1 tgttgtgttt tattatttgt tttttatcat taacttattt tgatgtttga gtccggcctt
       61 cgcaccaaaa gtatgtaaat agacctcaac tgaggtcttt ttttatgcct gaaatccgcg
      121 ccacgcaagg cttttccagc ttttctcctc aactgaagtc aacctgaacc aacccacatc
      181 aacaggcttg tgagtatact aatgagtata tttgccgatt cgatattgct tgtatactca
      241 cgagatacca atggaaggaa gaccattatg gccctaacgg atactaaagt tcgctctgcg
      301 aaacctgagg agaaagagta ttcacttgtt gacggcgacg gcatgtcctt acttgtaaaa
      361 cctggcggtt caaagtattg gcgatttcgt ttccgttttg gcggtaaaca acatttgatg
      421 gcgttcggcg tttacccgga tgtttcactg gcggatgcgc ggaagaaaag ggaagaggcc
      481 agaaagctgg tagctgctgg tatcgatcct cgtgagcata agcgtgctgt gaaagaagag
      541 caggcgaaag agattattac tttcgagaag gttgccagag agtggctcgt aaccaaccaa
      601 aaatggtcgg aagatcatgc taatcgtgta aaaaagagcc tggaggacaa tatcttcccg
      661 acaattggta ctcgcaacat tgctgaacta ggcacccgtg atctgttaat tcctattaaa
      721 gccgtggaga aatccgggcg tcttgaagtg gcttcccgcc ttcaacagcg caccacggcc
      781 atcatgcgtt atgcagtgca aagcgggtta attgattacg atccggcgca agagatgtcg
      841 ggggcggttg cttccagtaa tcgacagcat cgtccggcgc tggagttaaa gcgtatccct
      901 gagttgcttg acaaaataga cagctacacc ggcagaccgc taacccactg tacgacagaa
      961 ctcactctgc ttatctttat tcggtccagt aagctgcatt ttgctcgctg gtcagaaatc
     1021 gattttgaaa cgtcaatgtg gaccatccct cttgattggt attgcagtaa gcagagggtt
     1081 ggactggtac agtaaatatc ccgcataatc gtgccattca catttagaga tcctccggca
     1141 taatcaatct gccaatgaag gagatcgcta ttcgtaaagc cccttttact gagcatcaga
     1201 ttatcgccgt gattaaatcg cttgaatacg ggcgaactgt taaagatgtg gcttatctga
     1261 agccacttac tacaactgca agtccagata cggcggtatg gaattttctg atattaaaat
     1321 aatcaaggat cttgaggatg agaaccaacg tttcaaatag atgtttgctg atctcattat
     1381 ttggtcggga tttttaaaga tgtatggaac taaaacgggt ctattttcag agggatttaa
     1441 ctgaagcaga aattaataaa tcataattta taggtggctg gtatttaata ttcctgcatc
     1501 atatctctga tgcaggagca gagaatggct ataaattatt agtaaacaga atgtctatct
     1561 ctcacccaca aacccctcat aagggtaatt tttacgcacc tgcggcaacg caatagtttt
     1621 cccgtcaaaa tcccagaata atctctcaac gatccctccg ccgtttaccg catccgagac
     1681 cagtacccgc atattttcat ccaggccaat cgatccttta tcaaaggctt tatggtgtat
     1741 tgcgcaaagc gccagaccat tggggatttc acaagggccg ccgtgctgtt tccattttat
     1801 atgcgccgct tccagcgcga cggtggtgtc atcgtggcgc atattaaagc cacatattgc
     1861 gcactggtag ttataagcgc gtaatacatt tttgcgaaat aacgggtcgc gctgtttacg
     1921 ggtttgctgg agatcaaaac ccagctcgtc agcgatttct tcctgaatac tctcggtgaa
     1981 gtgcgcttcg agtatctgtt gcgccagagt attgatgagt tttttattgc cggttaccag
     2041 ggcgtagtgc tgttcatcga aaccgcctgc aacgtggtat tcgttcagtt ccttcactgg
     2101 cggttgtcgg ctgctccctg ctgttgagca acgctcggca ttgtgtaatt gccagattcc
     2161 gtcgccttgt aatcgccaga acggcatatc aggtcggtac tgtgaacgct gtggcccaaa
     2221 acgttccagt aaactgtgca gaggttcgta gatttccgtg ccataatcga aaaggcgcgg
     2281 atgtccattc aggtatcccg ctaatacgta tagcagtaac aatggtttat gcggcgcgcg
     2341 ctgttcaccc ttgtgccaga tttttatgtt ggcaattgcc tgctgtagcg atttactgga
     2401 agccattgat atcccaggca gtgaaataat tgcaatcatg ctcgcaaatt ttctgctgtt
     2461 caagcctgca agtgaagatc cattgctgtg ttgagggatc aaaagacaac aaaaacttat
     2521 tgaagaaata atcttctgct tttttgtcga aataatgagc attgatcttg tctatagctg
     2581 ctgaatcttt tccagcctcg ttgagatctt caaaatagcc attgaagatg ttctggttaa
     2641 caccgccagt aacataagcc ttgccgttgt gtacaaaagt cacattaccc gccatgccca
     2701 tcggcgcgtg cgacatcaat ttattgcttt attcattgtg actgtcttct gtctaccaag
     2761 ctttaacctt cacccatatg agggaagttt taagtatgag gcgaaccata gcatatgctt
     2821 ttatacctgt tacaccataa aaatcagaca agttatgcca taaactactt ttgtaatttg
     2881 agccacaaat cagggaaatt ttatttatct tccgtgaaag tcttaacttt cacggagttt
     2941 tagaatttac aattttgctg gcgtaaaggc tcctgtttgt atttctttaa ccagctcacc
     3001 cacggtatat attctccatc cttcgacttc ggcgggagta aggttaactg caattttaag
     3061 gcctggccat gcagcttcta cggttgcaat aatttcatgt tctgagtttg gaagatctaa
     3121 cccaggaaca ggaggttgaa agcctttatg ttgcatcata ttaatatcac catgcactga
     3181 tggatcagtc aggtcatata tttcattcca ggattctgct tttaataacc cttgttggga
     3241 acgtaaacgt tgaatgatag ccatctctgc ttgggtaacg aatatacctt cgtggagggg
     3301 ctgttttcga tggcaatcct tgcacagaac aagaaggttt tcatggtgat tatcatattt
     3361 gatgccattt ttatgatgga catggcacaa gtttttggcg gttgataaat tcacgccaca
     3421 atcattacaa acataatttg ccttttctcg tatttcttta gatatttcct tccagttttc
     3481 gacataccca ctatttgttt tgtcatagat acccttaggc atatgacgaa aacaagtact
     3541 ataatctgaa aaaaactcat ttaatgagaa tgatttaaaa atctctcttt ttcgttgttt
     3601 atcaatactt cctttataat taagtcgttc gaggcaattc atacataccc gtaaatcaac
     3661 atcctggctc cgacctgaac catcatcaat ttcgaataaa ccatctatgc ggttagttgc
     3721 atgatacctt tcaaatcgat ttttatggcg catttcatca agagttctgc accaggcaat
     3781 atgaaaacgt tttccttttt cgccatctac gatagccgca tcataacgcc ctgaatggtc
     3841 ccgaatatat aaaagaacct ggcggccatg ataagagata agcccgctac cagggtcaag
     3901 atcttcgata ctaacttctt tacccgtgga taactcaata tctataggat caaagtcaat
     3961 aggggattca tccagaaaaa aatcgactcc ttctggtaac attttcttcc ctaattgaat
     4021 aagttctgaa atgtcgatag ataatttcat ctcttactcc tgaataatct ttctaatctg
     4081 caactcggca tttgttacaa ctttgaaggt gacacgacga gagcgattag gatcctcctt
     4141 gccggtttta tcaagaatgg gatgtgcaga tgaataacct actgcggcaa atttactttt
     4201 aacccattgt tggtgtgtcg cgatattttt tatgtcataa acgtattgta atactgcacg
     4261 tgtacgacct tgcgatagtg ccatattatt aaaataagca atatcaggat tcgttgttcc
     4321 tgtccagtca gtacttgtgt gaccttcaat gcggacttca gtaatatgtt cctgataatt
     4381 atctagaact tttaggtagc gaggaaagaa gtcgtcaaga atgagtttaa actttggttt
     4441 tagctctgtg cttcctaagc caaataaaac atccggtgat ttaaatcgaa cctccagagt
     4501 ctgtttatcg atctctgcat cccagtcttg taaatccttt gcaaactcaa tatccagagc
     4561 attataaagc tgtaactgag cattctcgta ggctacggca acttctttaa ttttttcttt
     4621 ttcaatacgt acgtagtgca tataagcaat agatatgaac ataaaaacca tcatcagccc
     4681 agccattagg tccgacatgg atacccaatg ttcattatct tgatcttttt taggtaattg
     4741 tttccctata atcttatcca tgatcgatta cccgcgatgt ttattagcag aaattaatgc
     4801 ttgtaactgc cccataacta tttggtaatc tttaataagt ttttcatagt taccgacaaa
     4861 acctttgctg attgaaagca gagcattacc taatgattgc atttcacggt tcatttcacg
     4921 ttcagttgct tgctcaaaag catgtaactg ctcgttgatg gatagagcaa atttttcaat
     4981 tgattctctg acttgagaag tcagccctgc acttgcctta tcaaatgatg tctggattga
     5041 atcaaacata ttatcggcat tactggatag ttgatggtta atcttttcac cagtcttact
     5101 gaacatatca atcacattcg tcgtattttg ttccatattt ctatgcaatt ctttcgagtg
     5161 cgactctagt gatttcagga attcttcacc actttgtttc atttcagtaa ttgtttcacc
     5221 taacgtgctg gttagggttt cggaggaatt gctgacatca tgtgccatcg aggcaaatgc
     5281 ttgttgtgtt tgggtaaccg attgtctgaa tccttcggta ccttcatcca gggcaacgcg
     5341 cattgaatct gcattaagaa gtatttgctg gctggtttgc tcaagagatg cactaacatt
     5401 tgcagctccg gatttcagca gttcacccac ttcagccatt ttgttctgta tttcaggtaa
     5461 tacggttgta gctttatcgc ggatggcgac aaaggtttct aaatggcggg agagttcgct
     5521 gatttgatgt tggttcacct gaagcacttc acgcagttca gacatagcca gaggaatttc
     5581 tttacattct tcccaaatcc ctgcaaccgc agtttttgtt tcaaccaggg actcgacact
     5641 ttgttgatat tgttctgaca tctgctcaat ttgcgtttta taatttccct gccactcaac
     5701 aagttttttt acagaggcat caagagcttt aaagttttca ccaaactgtt cagttaaatt
     5761 ttgattaaaa tcaataatga cttgtcgcaa agcatcaata atttgttctg tagcaccttt
     5821 tgccattaga tctgcaaatt gttcaagttg ttcccaaagc ttattactga aatgagtgtg
     5881 attagctaat tgtgcctgcg aggaatcgct aatctcagta cgtagtaatt ttatttgagc
     5941 aataagagaa ccttcactat caccgttaat acccgagact aatttagtca gagtctggtt
     6001 ctgctctttt aattcatgat agatatgttc aggtgtaaca gattcagggt tattttctgc
     6061 taacgcactt cgtttattgg caaaaaagaa agcatccatt ccattaaata aaatggcaaa
     6121 aaacatacct acaatgcttg taatgaatgc tgtttttagg ccacctaata atacgggg
//
LOCUS       DAZFBR010000194         6085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFBR010000194 DAZFBR010000000
VERSION     DAZFBR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.194"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            108..1178
                     /gene="ygjJ"
                     /locus_tag="ACHJZ2_004697"
     CDS             108..1178
                     /gene="ygjJ"
                     /locus_tag="ACHJZ2_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO4570656.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            1195..3546
                     /gene="ygjK"
                     /locus_tag="ACHJZ2_004698"
     CDS             1195..3546
                     /gene="ygjK"
                     /locus_tag="ACHJZ2_004698"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO4570657.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
     gene            4072..>6085
                     /gene="fadH"
                     /locus_tag="ACHJZ2_004699"
     CDS             4072..>6085
                     /gene="fadH"
                     /locus_tag="ACHJZ2_004699"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO4570658.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT         1461 a         1639 c         1703 g         1282 t
ORIGIN      
        1 ttttccctct tcctggatga ggagagggaa aatagtttct gcctttatat ttttataatt
       61 acaacgataa aaggctgtac tttttcttta gctcatggat taacacaatg aaattaatca
      121 ctgcaccatg cagagcatta cttgctctgc cgttttgcta cgccttttct gcggcaggag
      181 aagaagcacg tccggcagaa catgacgaca caaaaacccc cgcaattacc tcgacatctt
      241 ctccttcatt tcgtttttac ggcgaattag gggttggtgg atatatggat ttagagggtg
      301 agaataaaca taaatacagc gacggcacct atattgaagg tggcctggag atgaagtacg
      361 gctcctggtt cggcctgatt tacggcgaag gctggaccgt gcaggccgac cacgacggca
      421 atgcctgggt gcctgaccat agctggggcg gtttcgaagg tgggattaac cgtttctatg
      481 gcggttatcg aaccaatgat ggcaccgaaa tcatgctcag tctgcgtcag gattcctcgc
      541 tggatgacct gcaatggtgg ggcgacttca cacccgatct gggctacgtc attcccaata
      601 cccgcgacat tatgaccgcg ctgaaggtgc agaacttaag cggcaacttt cgttatagcg
      661 tcaccgccac acctgccgga catcatgacg aaagcaaagc atggctacat tttggcaagt
      721 acgatcgcta cgacgacaaa tacacctatc cggcaatgat gaacggttac atccagtatg
      781 accttgccga aggcatcacc tggatgaatg gtctggaaat taccgacggc acaggacaac
      841 tctatctcac gggactgctt acccctaact ttgccgctcg cgcctggcac cataccggac
      901 gtgccgacgg gctggacgta ccgggaagtg aaagcgggat gatggtgagc gccatgtatg
      961 aagcgttaaa gggcgtttat ctctccaccg cttacaccta cgccaaacat cgccctgacc
     1021 acgctgacga tgaaaccacc tctttcatgc agtttggtat ctggtacgaa tatggcggcg
     1081 gacgtttcgc aacggctttt gatagccgct tctacatgaa aaatgcctct aacgatccca
     1141 gcgaccaaat cttcctgatg caatatttct actggtaata aggactgtaa tttcatgaaa
     1201 atcaaaacta ttttaacgcc agtaacctgc gctctgctga taagtttttc cgcccatgcc
     1261 actaacgccg acaattataa aaacgtgatt aaccgtactg gtgcgccgca gtacatgaag
     1321 gattacgatt acgacgatca ccagcgtttt aatccgtttt tcgatctcgg agcctggcat
     1381 ggtcatctgt tgccagacgg cccgaacacc atgggcggct ttccgggcgt tgcgctgctg
     1441 acggaagaat acatcaactt tatggccagc aatttcgacc gcctgaccgt ctggcaggac
     1501 ggcaagaaag tcaacttcac gctggaggca tacaatattc ccggcgcgct ggtgcaaaaa
     1561 ctgacagcaa aagatgtgca ggtcgaaatg actctgcgct tcgccacgcc gcgcacgtca
     1621 ctactggaaa ctaaaatcat cagcaataaa ccgctggatc tggtgtggga tggcgaactg
     1681 ctggaaaaac tggaagcgaa agaagggaaa ccgctttccg ataaaaccat tgctggcgaa
     1741 taccctgact accagcgcaa aatcagcgcc acccgtgatg gcctgaaagt cacctttggc
     1801 aaagtgcgcg ccacctggga tctactgacc tccggtgaat cagaatatca ggtgcataaa
     1861 tccctgccgg tgcagactga aatcaacggc aatcgcttta ccagtaaggc gcatatcaac
     1921 ggttcgacga cgctctatac cacctattcc catctgctga cctctcagga agttagcaaa
     1981 gagcaaatgc agatccgcga tattctggcg cgtccggcgt tttatctcac cgcctcgcag
     2041 caacgctggg aagagtatct gaagaaaggg ttaaccaatc cggatgcgac gccggaacag
     2101 acacgcgtcg cggtgaaagc gatcgaaacg ctcaacggta actggcgctc gcctgggggt
     2161 gcggtgaaat ataacaccgt cacgccgtcg gtgaccgggc gctggttctc cggcaatcag
     2221 acctggccgt gggatacctg gaagcaggcg tttgcgatgg cgcatttcaa tccggacatc
     2281 gccaaagaga atatccgcgc ggtcttctcc tggcagatcc agcctggcga cagcgtgcgt
     2341 ccgcaggatg tgggctttgt ccccgacctg atagcgtgga atcttagccc cgagcgtggt
     2401 ggcgatggtg gcaactggaa cgaacgaaat accaaaccca gccttgccgc ctggtcggtg
     2461 atggaagtgt acaacgtcac ccaggataaa acctgggtgg cagagatgta cccgaaactg
     2521 gtggcctatc acgactggtg gttacgtaac cgcgatcaca acggcaacgg cgtgccggaa
     2581 tatggcgcga cccgcgacaa agcccacaac actgagagcg gcgagatgct gtttacggtg
     2641 aagaaaggca acaaagaaga gacgcagtct gggctgaaca actacgcccg cgtggtggag
     2701 aaaggccagt acgacagtct ggaaattccg gcacaggttg ctgcatcgtg ggaatcgggg
     2761 cgtgatgatg ccgccgtctt tgggtttatc gacaaagaac agctggataa atatgtcgca
     2821 agcggcggca aacgtagcga ctggacagtg aaattcgccg aaaaccgcag tcaggacgga
     2881 acgttgctgg ggtactcgct attgcaggag tcggtggatc aggccagcta tatgtacagc
     2941 gataaccatt atctggcgga gatggcgacg attctcggta aaccggaaga ggctaagcgc
     3001 tatcgccagt tggcacagca gctcgcggac tacatcaaca cctgtatgtt cgacccggct
     3061 acgcagtact tctatgacgt gcgtattgaa gataaaccgc tggcgaacgg ctgcgcgggc
     3121 aaaccgattg ttgagcgcgg taaagggccg gaaggctggt cgccgctgtt taacggtgcg
     3181 gcaacgcagg ccaatgccga cgcggtggtg aaggtgatgc tcgatcctaa agagttcaat
     3241 acctttgttc cgctgggaac ggcggcgtta accaatccgg cctttggcgc tgatatctac
     3301 tggcgcgggc gcgtatgggt ggatcagttc tggtttggtc tgaaagggat ggagcgttac
     3361 ggttatcgcg atgatgccct gaaactggcg gatacgttct tccggcacgc caaagggtta
     3421 accgccgatg gcccaattca ggaaaattac aacccgctga caggcgcaca gcaaggcgca
     3481 ccaaatttct cctggagtgc cgcacatttt tatatgttgt ataacgattt tttccgtaag
     3541 caataatttg caacggctgg cggatgcggc gtgaacgcct tatccgccct acatattcgt
     3601 tcccgtaggc tggataagac gcgacaagcg tcgcatccgg catctgcacc tcgccaccgg
     3661 cggatgctgc gtgaacgcct tatccgccct acatgttcgt tcccgtaggc tggataagac
     3721 gcgacaagcg tcgcatccgg catctgcacc tcgccactgg cggatgcggc gtgaacgcct
     3781 tatccgccct acatgttcgt tcccgtaggc tggataagac ccgacaagcg tcgcatccgg
     3841 cgtctgcacc gcgccactgg cggatgcggc gtgaacgcct tatccgccct acatgtgcaa
     3901 tcccccaggt cggatagggc ccggcaagcg tcgcatccgg cgttaccacc gggcgtattc
     3961 tttttgaatc ccatcacaaa ccccgcactc cccttttccc ttttctccgg cgacggctaa
     4021 attagaactc atccgaccac ataacaatta ttttacatac tggacaattt tatgagctac
     4081 ccgtcgctgt tcgccccgct ggatttaggt tttaccacgt taaaaaaccg cgtgttgatg
     4141 ggttcaatgc acaccgggct ggaggaatac ccggacggtg ctgaacggct ggcagcgttt
     4201 tatgccgaac gcgcccgtca cggcgtggcg ctgattgtta gcggcggcat tgcaccagat
     4261 ttaacaggcg ttggcatgga aggcggcgca atgctcaatg acgccagcca gatcccacac
     4321 catcgcacca ttaccgaagc ggtacatcag gaaggcggca aaatagccct gcaaattttg
     4381 cataccgggc gctacagcta ccaaccgcat ctggtcgcac cgtccgcatt gcaggccccc
     4441 atcaaccgtt tcgttcctca tgaactcacc catgaagaga tcctgcaact gatcgacgat
     4501 ttcgcccgct gcgcgcaact ggcacgggag gcaggatacg acggtgtaga ggtaatgggt
     4561 tccgaagggt atttgatcaa cgaatttctg acgctgcgca ccaatcagcg cagtgaccag
     4621 tggggcggcg attaccacaa ccggatgcgg tttgccgttg aagtggtgag tgcggtacgc
     4681 gaacgcgttg gcaacgactt cattattatc taccgactgt cgatgctcga cctggtcgaa
     4741 ggcggcggga cttttgccga aacggtagag ctggcgcagg ccattgaagc ggcgggcgcg
     4801 accattatca acaccggcat tggctggcat gaagcgcgta ttccgaccat cgccacgccc
     4861 gtgccacgcg gcgcatttag ctgggtcacg cgcaaactga aaggtcacgt ctcgctgccg
     4921 ctggtgacca ccaaccggat taacgatccg caggttgccg acgatattct ctcgcgcggc
     4981 gatgccgata tggtatcgat ggcgcgaccg tttcttgcgg atgcggaact gctgtcaaaa
     5041 gcgcaatcag ggcgagccga tgagatcaac acctgcattg gctgcaacca ggcgtgtctc
     5101 gatcagatct tcgttggcaa ggtcacctcg tgtctggtga atcctcgcgc ctgccatgaa
     5161 accaaaatgc cgatccttcc cgccatgcag aaaaaaaatc tggcggtggt cggtgcggga
     5221 cctgctgggc tggcgtttgc cattaacgcg gcggcgcgtg gtcatcaggt aacgttgttt
     5281 gacgcgcaca gtgagatcgg cgggcagttt aatatcgcca aacagatccc cggcaaagag
     5341 gagttttacg aaacgctgcg ctattaccgc cggatgatcg aagtgacggg cgtgacgcta
     5401 aaactcaatc acaccgtgac ggcggatcag ttacaggcgt tcgatgaaac gatcctcgcc
     5461 agtgggatcg tgccgcgcac tccgcccatc gacgggatcg atcatccgaa ggtattgagt
     5521 tatctcgatg tactgcgcga caaagcgccg gtgggcaaca aagttgccat catcggttgt
     5581 ggcgggattg gttttgatac ggcgatgtat ttaagtcagc cgggcgaatc caccagccag
     5641 aatatcgccg ggttctgtaa tgaatggggg atcgacagca gcctacaaca ggctggtggc
     5701 ttaagcccgc agggaatgca gatcccgcgt agcccacggc agattgtgat gctccagcgc
     5761 aaagccagca aaccgggaca ggggttaggc aaaaccaccg gctggatcca tcgcaccacc
     5821 ctgctctcgc ggggtgtgaa aatgatccca ggcgtaagtt atcagaagat tgacgatgac
     5881 gggctgcatg tggtgatcaa cggcgaaacg caggtattag cagtggacaa tgtggtgatc
     5941 tgcgcagggc aagagccaaa ccgcgcattg gcgcagccgc tgattgatag cgggaaaacg
     6001 gtgcatttaa ttggcggctg cgatgtggct atggagctgg acgcacgacg ggcaattgcc
     6061 cagggaacac ggctggcgct ggaga
//
LOCUS       DAZFBR010000195         6072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFBR010000195 DAZFBR010000000
VERSION     DAZFBR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.195"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..253
                     /locus_tag="ACHJZ2_004700"
     CDS             <1..253
                     /locus_tag="ACHJZ2_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4570659.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTIGEENFWIDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            265..843
                     /locus_tag="ACHJZ2_004701"
     CDS             265..843
                     /locus_tag="ACHJZ2_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4570660.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETRVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            840..1235
                     /locus_tag="ACHJZ2_004702"
     CDS             840..1235
                     /locus_tag="ACHJZ2_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4570661.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1243..1983
                     /locus_tag="ACHJZ2_004703"
     CDS             1243..1983
                     /locus_tag="ACHJZ2_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4570662.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1999..2421
                     /locus_tag="ACHJZ2_004704"
     CDS             1999..2421
                     /locus_tag="ACHJZ2_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4570663.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2403..2837
                     /locus_tag="ACHJZ2_004705"
     CDS             2403..2837
                     /locus_tag="ACHJZ2_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4570664.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2830..5379
                     /locus_tag="ACHJZ2_004706"
     CDS             2830..5379
                     /locus_tag="ACHJZ2_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4570665.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGEEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            5376..5705
                     /locus_tag="ACHJZ2_004707"
     CDS             5376..5705
                     /locus_tag="ACHJZ2_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4570666.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5705..>6072
                     /locus_tag="ACHJZ2_004708"
     CDS             5705..>6072
                     /locus_tag="ACHJZ2_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4570667.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
BASE COUNT         1409 a         1488 c         1987 g         1188 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgt gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag accggatttc
      181 gacggatgat ggcggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa
      241 ccgtcgtcgc tgaagggggg atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac
      301 ctcagtcgta tcagcaaaac ggcggtgcct ggtgcgtcag caatggccat taaccgcgtt
      361 gcttcatccg cgatatcgca gtcggcgtca caggttgccc gtgagacaag ggtacgccgg
      421 aaactggtaa aggaaagggc caggctgaaa agggccacgg ttaaaaatcc gcaggccaga
      481 atcaaggtta accgggggga tttgcccgta atcaagctgg gtaacgcgcg ggttgtcctg
      541 tcccgacgca ggcgtcgtaa aaaggggcag cgttcatccc tgaaaggtgg cggcagcgtg
      601 cttgtggtgg gaaaccgtcg tattcccggc gcgtttattc agcaactgaa aaatggccgg
      661 tggcatgtca tgcagcgtgt ggccgggaaa aaccgttacc ccattgatgt ggtgaaaatc
      721 ccgatggcgg tgccgctgac cacggcgttt aaacagaata ttgagcggat acggcgtgaa
      781 cgtcttccga aagagctggg ctatgcgctg cagcatcaac tgagaatggt aataaagcga
      841 tgaaacatac tgaactccgt gcagccgtac tggatgcact ggagaagcat gacaccgggg
      901 cgacgttttt tgatggtcgc cccgctgttt ttgatgaggc ggattttccg gcagttgccg
      961 tttatctcac cggcgctgaa tacacgggcg aagagctgga cagcgatacc tggcaggcgg
     1021 agctgcatat cgaagttttc ctgcctgctc aggtgccgga ttcagagctg gatgcgtgga
     1081 tggagtcccg gatttatccg gtgatgagcg atatcccggc actatcagat ttgatcacca
     1141 gtatggtggc cagtggctat gactaccggc gcgacgatga tgcgggcctg tggagttcag
     1201 ccgatctgac ttatgtcatt acctatgaaa tgtgaggacg atatgccaac accaaatcct
     1261 ctggcaccgg tgaaaggggc cgggaccaca ctgtgggttt ataacgggaa cggcgaccca
     1321 tatgcaaacc cgctttcaga caatgactgg tcgcgtctgg caaaggttaa agacctgacg
     1381 cccggcgaac tgaccgctga gtcctatgac gacagttatc tcgatgatga agatgcggac
     1441 tgggccgcga ccggacaggg gcagaaatcc gctggagata ccagcttcac gctggcgtgg
     1501 atgcccggag agcaggggca gcaggcgctg ctggcgtggt ttaatgaagg tgatacccgt
     1561 gcctataaaa tccgcttccc gaacggcacg gtcgatgtgt tccgcggctg ggtcagcagt
     1621 atcggtaagg cggtgacggc gaaggaagtg atcacccgca cggtgaaagt caccaacgtg
     1681 ggacgtccgt cgatggcaga agatcgcagc acggtaacag cggcaaccgg catgaccgtg
     1741 acgcctgcca gcacctcggt ggtgaaaggg cagagcacga cgctgaccgt ggcattccag
     1801 ccggaaggcg caaccgacaa gagcttccgt gcggtgtctg cggataaaac aaaagccacc
     1861 gtgtcggtca gtggtatgac catcaccgtg aaaggtgttg ctgcaggcaa ggtcaacatt
     1921 ccggtcgtat ccggtaatgg tgagtttgct gcggttgcag aaatcaacgt caccgccagt
     1981 taatccggag aatcagcgat gttcctgaaa accgaatcat ttgaacataa cggcgtgacc
     2041 gtcacgcttt ctgaactgtc agccctgcag cgtattgagc atctcgccct gatgaaacgg
     2101 caggcagaac aggcggagtc agacagcaac cggaagttta ctgtggaaga cgccatcaga
     2161 accggcgctt ttgtggtggc gatgtccctg tggcataacc atccgaagaa gacgcagatg
     2221 ccgtccatga atgaagccgt taaacagatt gagcaggaag tgcttaccac ctggcccgcg
     2281 gaggcaattt ctcatgctga aaacgtggtg taccggctgt ccggtatgta tgagtttgtg
     2341 gtgaatgatg cccctgaaca ggcagaggac gccgggcctg cagagcctgt ttctgcggga
     2401 aagtgttcga cggtgagctg agttttgccc tgaaactggc gcgcgagatg gggcgacccg
     2461 actggcgtgc catgcttgcc gggatgtcat ccacggagta tgccgactgg caccgctttt
     2521 acagtaccca ttattttcat gatgttctgc tggatatgca cttttccggg ctgacgtaca
     2581 ccgtgctcag cctgtttttc agcgatccgg atatgcatcc gctggatttc agtctgctga
     2641 accggcgtga ggctgacgaa gagcctgaag atgatgtgct gatgcagaaa gcggcagggc
     2701 ttgctggagg cgttcgtttt ggcccggacg ggaatgaagt tatccccgct tccccggatg
     2761 tggcggacat gacggaggat gacgtaatgc tgatgacagt atcagaaggg atcgcaggag
     2821 gagtccggta tggctgaacc ggtaggcgat ctggtcgttg atttaagtct ggatgcggcc
     2881 agatttgacg agcagatggc cagagtcagg cgtcattttt ccggtacgga aagtgatgcg
     2941 aaaaaaacag cggcagtcgt tgaacagtcg atgagccggc aggcgctggc tgcacagaaa
     3001 gcgggaattt ccgtcgggca gtataaagcc gccatgcgta tgctgcctgc gcagttcact
     3061 gacgtggcca cgcagcttgc aggtgggcaa agtccgtggc tgatcctgct gcaacagggg
     3121 gggcaggtga aggactcctt cggcgggatg atccccatgt tccgggggct tgccggtgcg
     3181 atcaccctgc cgatggtggg ggccacctcg ctggcggtgg cgaccggtgc gctggcgtat
     3241 gcctggtatc agggcaactc aaccctgtcc gatttcaaca aaacgctggt cctttccggc
     3301 aatcaggccg ggctgacggc agatcgtatg ctggtcctgt ccagagccgg gcaggcggca
     3361 gggctgacgt ttaaccagac cagcgagtca ctcagcgcac tggttaaggc gggggtaagc
     3421 ggtgaggctc agattgcgtc catcagccag agtgtggcgc gtttctcctc tgcatcaggc
     3481 gtggaggtgg acaaggtcgc tgaagccttc gggaagctga ccactgaccc gacgtcgggg
     3541 ctgacagcga tggcgcgcca gttccataac gtgacggcgg agcagattgc gtatgttgct
     3601 cagttgcagc gttccggcga agaagccggg gcattgcagg cggcgaacga ggccgcgacg
     3661 aaagggtttg atgaccagac ccgccgcctg aaagagaaca tgggcacgct ggaaacctgg
     3721 gcagacagga cagcacgggc attcaaatcc atgtgggatg cggtgctgga tattggtcgt
     3781 cctgataccg cgcaggagat gctgattaag gcagaggccg cgtttaagaa agcggacgat
     3841 atctggaatc tgcgcaagga tgattatttt gttaacgatg aagcgcgggc gcgttactgg
     3901 gatgatcgtg aaaaggcccg tcttgcgctt gaagccgccc gaaagaaggc tgagcagcag
     3961 agtcaacagg acaaaaatgc gcagcaacag agcgataccg aagcgtcacg gctgaaatat
     4021 accgaagagg cgcagaaggc ttacgaacgc ctgcagacgc cgctggagaa atataccgcc
     4081 cgtcaggaag aactgaataa ggcactgaaa gacgggaaaa tcctgcaggc agattacaac
     4141 acgctgatgg cggcggcgaa aaaggactat gaagcgacgc tgaaaaagcc gaaacagtcc
     4201 ggcgtgaagg tgtctgcggg cgatcgtcag gaagacagtg ctcatgctgc cctgctgacg
     4261 cttcaggcag aactccggat gctggagaag catgccggag cgaatgagaa aatcagccag
     4321 cagcgccggg atttgtggaa ggcagaaagt cagttcgcgg tactggagga ggcggcacaa
     4381 cgtcgccagc tgtccgcaca ggagaaatcc ctgctggcgc ataaagacga gacgctggag
     4441 tacaaacgcc agctggctgt acttggcgac aaggtcacct atcaggagca cctgaacgcg
     4501 ctggcacagc aggcggataa attcgcacag caacaacggg caaaacgggc cgccattgat
     4561 gcaaaaaacc gggggctgac tgaccggcag gcagcgcggg aagccacgga acagcgcctg
     4621 aaggaacagt atggcgataa tcctctggcg ctgaataacg tcatgtcaga gcagaaaaag
     4681 acctgggcag ctgaagacca gcttcgcggg agctggatgg caggcctcag gtcaggctgg
     4741 agtgagtggg aagagagcgc cacggacagt atgtcgcagg ttaaaagtgc tgccacgcag
     4801 acctttgatg gtattgcaca gaatatggcg gcgatgctga ccggcagtga acagaactgg
     4861 cgcagcttca cccgttccgt gctgtccatg atgacagaaa ttctgcttaa gcaggcaatg
     4921 gtggggattg tcggaagtat aggcagcgcc attggcggcg gcgcatcagc gtcaggcggt
     4981 acagccattc aggcagctgc ggcgaaattc cattttgcgg ccggagggtt tacgggaacc
     5041 ggcggcaaat atgagccagc ggggattgtt caccgtggtg aatttgtctt cacgaaggag
     5101 gcaaccagcc ggattggcgt aggaaatctt taccggctga tgcgcggcta tgccaccggc
     5161 ggttatgtcg gtacaccggg cagcatggcg gacagccggt cgcaggcgtc cgggacgttt
     5221 gagcagaata accatgtggt gattaacaac gacggcacga acggtcagat agggccacag
     5281 gcactgaagg cggtttatga cgtagcccgt aaggcggcaa tggatgttgt gaccgggcag
     5341 atgcgcgatg gtggtctgtt ctccggaggt ggacgatgaa aaccttccgc tggaaagtga
     5401 aacccggtat ggatgtggct tcggcccctt ccgtaagaaa ggtgcgcttt ggtgatggct
     5461 attcccagcg agcgcctgcc gggctgaatg ccgacctgaa aacgtacagc gtgacgcttt
     5521 ctgttccccg ttgggaggcc acggcgcttg agtcgtttct ggctgagcac gggggctgga
     5581 aggcctttct gtggacgccg ccttatgagt ggcggcagat aaaggtgacc tgcgcaaaat
     5641 ggtcgtcgcg ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa caggtggtga
     5701 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcggc
     5761 cagcgtggtg ctctgggaaa tcgatctgac agaggtcggt ggagaacgtt attttttctg
     5821 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta
     5881 tcccattcag gggagtggtt ttgaactgaa tggcaaaggc accagtacgc gccccacgct
     5941 gacggtttct aacctgtacg gtatggtcac cgggatggcg gaagatctgc agagtctggt
     6001 cggcggaacg gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt
     6061 caacggaaac ag
//
LOCUS       DAZFBR010000196         6035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFBR010000196 DAZFBR010000000
VERSION     DAZFBR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.196"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..780
                     /gene="espX2"
                     /locus_tag="ACHJZ2_004709"
     CDS             <1..780
                     /gene="espX2"
                     /locus_tag="ACHJZ2_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO4570668.1"
                     /translation="LSSDNSGRVMRLSHQALISMLEPEVKKKTIWNNYSIYPSLQDTH
                     EVVRDDPETICMRAFPLFAKGWEYAQKNKKHQLILNALGFKGYIRDIFMSAIMRKTDF
                     VLECNNQPTELNSSFSSLMNDSDQWQQHTLKDKHYANLLTMLDLNDASESDKSKIFFC
                     LSAVFANISHSNVFNGIPDASKTLKRYAFALLAKAHSLDESMISNQTFNTYKTVLLDF
                     NNLSNEEANQLRISSLYRDMVRYAQYRFSKVLSEWTPDAWL"
     gene            1064..3214
                     /gene="dinG"
                     /locus_tag="ACHJZ2_004710"
     CDS             1064..3214
                     /gene="dinG"
                     /locus_tag="ACHJZ2_004710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO4570669.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3242..4204
                     /gene="ybiB"
                     /locus_tag="ACHJZ2_004711"
     CDS             3242..4204
                     /gene="ybiB"
                     /locus_tag="ACHJZ2_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO4570670.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4345..5430
                     /gene="hcxB"
                     /locus_tag="ACHJZ2_004712"
     CDS             4345..5430
                     /gene="hcxB"
                     /locus_tag="ACHJZ2_004712"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO4570671.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5497..5556
                     /gene="ybiE"
                     /locus_tag="ACHJZ2_004713"
     CDS             5497..5556
                     /gene="ybiE"
                     /locus_tag="ACHJZ2_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO4570672.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5659..5919)
                     /gene="ybiJ"
                     /locus_tag="ACHJZ2_004714"
     CDS             complement(5659..5919)
                     /gene="ybiJ"
                     /locus_tag="ACHJZ2_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO4570673.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
BASE COUNT         1498 a         1452 c         1640 g         1445 t
ORIGIN      
        1 ttatcatccg ataactctgg gcgagtaatg cgcttatcgc accaggcgtt aatctcaatg
       61 cttgagccag aggttaagaa aaagactatc tggaataatt actctattta tccatctttg
      121 caggataccc atgaagttgt cagagatgat ccagaaacga tttgtatgcg tgcatttcct
      181 ttatttgcaa aaggatggga atatgcgcag aaaaataaga aacatcaatt gatcctcaat
      241 gctcttggat tcaaaggtta tattcgtgat atatttatgt cagcaataat gcgaaaaaca
      301 gattttgtgc tcgaatgcaa taatcaaccg acagaactta attcatcatt ttcctctttg
      361 atgaacgatt cagatcaatg gcaacagcat actctgaaag ataaacatta cgctaatcta
      421 ttaacgatgc tggatctcaa tgatgccagt gaaagtgata agtctaaaat atttttctgc
      481 ctgtctgccg tatttgcaaa tatttctcac agtaatgtct ttaacggtat tcctgatgcg
      541 tcaaaaactt taaaacgtta tgcatttgct ttattggcga aagctcattc tttagatgaa
      601 agtatgataa gcaatcaaac ttttaataca tacaaaacgg tattgcttga tttcaataat
      661 ctgtctaatg aagaggccaa ccagttgcgc ataagttcgt tatatcgtga tatggtccgc
      721 tatgcacaat atcgtttttc taaagtttta tccgagtgga cgccagacgc atggctgtaa
      781 agatcgcagc agaataatgg cattgatggc cctggctcag gccagggtcg gcaataccgg
      841 ttcctgcgaa gcgatgacgg cggcaattgt aaccacgaaa tgcggtatca cttgctgttt
      901 tctgccctga agactggcgc tcactgtgcc gtcgaatttt caatcaaata tattgcgaga
      961 gggcgtgttt gcgagccgct ttccagaaac agaaaaacca ttacccctga aaaccgaaaa
     1021 atgccacaat attggctgtt tatacagtat ttcaggtttt ctcatggcat taaccgccgc
     1081 gcttaaagcg caaattgccg cctggtataa ggcgcttcag gaacagatcc ccgactttat
     1141 tccccgtgcg ccgcagcggc agatgattgc ggacgtcgcc aaaacgctgg ccggagaaga
     1201 agggcggcat ctggcgattg aagcccccac tggcgttggg aaaacgctct cctatttgat
     1261 tcccggcatc gccattgccc gcgaagagca aaaaacgctg gtggtgagta ccgccaacgt
     1321 ggcattgcag gatcagatct acagcaaaga tttgccactg ctgaaaaaga tcattcccga
     1381 tcttaaattc actgccgctt ttgggcgtgg gcgctacgtt tgcccgcgta atctgacggc
     1441 gcttgccagc actgagccca cgcaacagga tctgctggcg tttcttgacg acgaactgac
     1501 gccaaataat caggaagagc aaaaacgttg tgcgaagctg aagggcgatc tcgacactta
     1561 taaatgggat ggtctgcgcg atcatacgga tatcgctata gatgacgatc tctggcgtcg
     1621 tttaagtacc gacaaagcca gctgcctcaa ccgcaactgt tactactatc gcgaatgccc
     1681 gttttttgtc gctcgtcggg aaattcagga agcggaagtg gtggtggcaa accatgcgct
     1741 ggtgatggcg gcgatggaaa gcgaagccgt attgcctgac ccgaaaaatt tactgctggt
     1801 gctggacgaa ggccatcacc tgccagatgt ggcgcgggat gcgctggaga tgagtgccga
     1861 aatcaccgcg ccatggtatc ggctacagct ggacttgttc acgaaactgg tcgctacctg
     1921 catggagcag tttcgcccga agaccatccc gccgttggcg atccctgaac gtttgaatgc
     1981 gcattgtgaa gagctgtatg agcttatcgc ctcattaaac aacattctca atctctacat
     2041 gcctgccggg caggaagcag aacaccgttt tgcgatgggc gaactgcctg atgaagtgct
     2101 ggagatctgc cagcggctgg caaaactcac cgagatgctg cgtggtctgg cggagttatt
     2161 tcttaacgat ttaagtgaga aaaccggcag ccatgacatt gtacgtctgc atcggttgat
     2221 tttgcagatg aaccgcgcgt tggggatgtt cgaggcgcaa agcaaactct ggcggctggc
     2281 ttcgctggcg caatcttccg gtgcaccggt gaccaaatgg gcgacgcggg aagagcgcga
     2341 agggcagcta cacctctggt ttcactgcgt gggaatacgt gtcagcgatc agctggaaag
     2401 gctgctgtgg cgcagtattc cgcacattat tgtcacctcc gcaaccttgc gttcgctgaa
     2461 cagtttttcg cgtttgcagg agatgagcgg gctgaaagag aaagcgggcg accgttttgt
     2521 ggcgctggat tcccccttta accactgcga acagggcaaa attgttattc cccggatgcg
     2581 cgttgagcct tccatcgaca acgaagaaca gcatattgct gaaatggcgg cctttttccg
     2641 tgagcaggtg gagagcaaaa aacatctcgg tatgttggtg ctgtttgcca gcgggcgtgc
     2701 gatgcagcgc tttctcgact atgtgacgga tttacgtctg atgttgctgg tgcagggcga
     2761 tcagccgcgt taccgtttag ttgaactgca ccgcaaacgc gtcgccaacg gtgagcgtag
     2821 tgtgctggtg ggcttacagt catttgccga agggcttgat ttgaaaggtg atatgctcag
     2881 ccaggtgcat atccacaaaa tcgcttttcc gcctatcgac agcccggtgg tgatcactga
     2941 aggggaatgg ctgaaaagcc tcaaccgcta tccatttgag gtgcaaagcc tgccgagcgc
     3001 ctcgtttaac ctgattcagc aggttgggcg actgattcga agtcacggtt gctggggcga
     3061 agtggtgatt tacgataaac gcttgctgac caaaaattat ggcaagcgac tactggatgc
     3121 attaccggta tttccgatag agcaaccgga agtccctgaa ggtatagtta aaaagaaaga
     3181 aaaaacgaaa tccccacgcc gtcggcggcg ttaatgatgt gagtcaggta aggagtcgta
     3241 aatggactat cgcaaaatca ttaaagagat cgggcgcggg aaaaaccacg cgcgcgattt
     3301 agaccgggat actgcgcgcg gtctgtatgc ccatatgctc aatggtgaag tccctgacct
     3361 tgagttgggc ggtgtattga ttgcgctgcg tatcaaaggg gaaggggaag cagagatgct
     3421 cggcttttac gaagccatgc aaaatcacac catcaagctg acgccgcctg caggcaagcc
     3481 gatgccgatt gtcatcccca gttacaacgg cgcgcgtaaa caggccaacc tgacgccgtt
     3541 gttggcgatt ctcctgcaca aactcggttt tcctgtggtg gttcacgggg ttagcgaaga
     3601 tccaactcgc gtgctgaccg aaaccatttt cgaactgatg ggtattacgc caacgttgca
     3661 cggcgggcag gcgcaggcga agctcgacga acatcaaccg gtgtttatgc ctgtcggcgc
     3721 gttttgcccg ccgctggaaa aacaactggc gatgcgctgg cggatgggcg tgcgtaacag
     3781 tgcacatacc ctggcgaaac tggcgacgcc atttgccgaa ggtgaggcac tgcgtctttc
     3841 cagcgtttcg catccggaat acatcgggcg agtcgcgaag ttctttagcg atatcggcgg
     3901 gcgggcgtta ctaatgcatg gtactgaagg cgaagtgtat gctaatccgc agcgctgccc
     3961 acagatcaat ctcattgatc gtgaagagat gcgggtgctg tatgaaaaac aggaccctgc
     4021 tggaagcgag ttactgccac aagcaaaaga tccggaaacc acggcgcagt ggattcagcg
     4081 ttgccttgct ggccgcgaac cgattcccga atcgctgaaa atccagatgg cttgctgcct
     4141 ggtggctacg ggtgaagcgg caactatcag cgatggcctg gcgcgcgtta atcaggcatt
     4201 ttaattcttt tctcagccgg atgagccatg tcatccggct tttaccccac cgcaatatga
     4261 aattcctgca tctttattga ccttcccgcg cccggcgtgc agcataaaaa aacaacaaac
     4321 acataacata aacaggagtt aaccatggaa agtggtcatc gctttgatgc tcagacgctg
     4381 cacagcttta ttcaggctgt atttcgtcag atgggtagcg aggaacaaga agcgaaatta
     4441 gttgccgatc atttaatcgc ggcaaacctg gcagggcatg attcacatgg tattggcatg
     4501 atcccaagct atgtacgctc ctggagtcag gggcacctgc aaattaacca tcatgccaaa
     4561 atcgttaaag aggcgggggc ggcggtcacg ctcgatggcg atcgcgcatt tggtcaggtc
     4621 gcggcacatg aagcgatggc gctggggatt gagaaagcgc atcagcacgg tattgctgcc
     4681 gtggcgctac ataactcgca tcatatcggc cgtatcggtt actgggcgga gcagtgtgca
     4741 gcggcggggt ttgtctctat ccactttgtt agcgtggtcg gtattccaat ggtcgcaccg
     4801 ttccacggtc gcgacagccg ctttggcacg aatccgttct gtgtggtttt cccacgtaaa
     4861 gatgattttc cgctgttgct tgattacgcc accagcgcca ttgcatttgg caaaacccgc
     4921 gtcgcctggc ataaaggcgt ccccgtgccg ccaggttgcc tgattgacgt taacggcgtg
     4981 ccgacgacca atccggcggt aatgcaggag tcgccgttgg gttcgctgtt gacctttgcc
     5041 gaacataaag gctacgccct ggcggcaatg tgtgaaattc ttggcggggc gctttctggc
     5101 ggtaaaacga cgcatcagga aacgttacaa accagtcccg atgccattct taactgcatg
     5161 accactatca tcatcaaccc ggaactgttc ggcgcgccgg attgtagcgc gcagaccgaa
     5221 gcctttgccg agtgggtgaa agcctcgccg catgatgacg ataagccgat tttgctcccg
     5281 ggcgagtggg aagtgaacac gcgtcgcgaa cggcaggagc aggggattcc attggatgcg
     5341 ggaagctggc aggccatttg tgatgcggcg cggcagattg gtatgccgga agagacgttg
     5401 caggctttct gtcagcagtt agccagctaa aaaaaagccc gtccagtggc ggacgggcaa
     5461 acaagggtaa cataggatca atgagggtta gagcatatgc gtctgtcggc aaacagacag
     5521 ggaaatactt gtgcaggacg tagcgtaaac gcctgatccg tccaacggtt cggattttgt
     5581 aggcctgata agacgcggta gcgtcgcatc aggcatctgt tgccggatgc ggcgtaaacg
     5641 ccttatccgt cctacgaatt acttataaat taccgcagta ccgcttaatt tattgttgtt
     5701 ggtggcggaa gtgatgctat atccactggc accggctgcc gccgctttct cagccagttt
     5761 cgcttccagg gcatcgaggg tggatgcgcc atcggcagaa accacgccga ttttattcat
     5821 gttctgtgcc tgggatgccg ttaccggttc cgcggcaaac acgccaaatg acagagctga
     5881 aagagccata gcagcaacaa cagtattgat agttttcata attaatctct cgcaggtgat
     5941 ttttgtataa gtgacgttgt ttcgtcgatg tgatgagtat cacgtttttt tacgagagat
     6001 aaaatcgaag agaattgacc gccttgttca aataa
//
LOCUS       DAZFBR010000197         5697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFBR010000197 DAZFBR010000000
VERSION     DAZFBR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.197"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(99..464)
                     /locus_tag="ACHJZ2_004715"
     CDS             complement(99..464)
                     /locus_tag="ACHJZ2_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4570674.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            506..733
                     /locus_tag="ACHJZ2_004716"
     CDS             506..733
                     /locus_tag="ACHJZ2_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4570675.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
     gene            complement(1102..1326)
                     /locus_tag="ACHJZ2_004717"
     CDS             complement(1102..1326)
                     /locus_tag="ACHJZ2_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570676.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
     gene            complement(1323..1817)
                     /locus_tag="ACHJZ2_004718"
     CDS             complement(1323..1817)
                     /locus_tag="ACHJZ2_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4570677.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGT
                     VREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQVEGAQDYIRTQCTKQAFYY
                     PEDV"
     gene            complement(1819..1956)
                     /locus_tag="ACHJZ2_004719"
     CDS             complement(1819..1956)
                     /locus_tag="ACHJZ2_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570678.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKYILIWLVFVIMASGYFIGQIRW
                     W"
     gene            complement(2116..2649)
                     /locus_tag="ACHJZ2_004720"
     CDS             complement(2116..2649)
                     /locus_tag="ACHJZ2_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4570679.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(2654..2869)
                     /locus_tag="ACHJZ2_004721"
     CDS             complement(2654..2869)
                     /locus_tag="ACHJZ2_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4570680.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(2946..3218)
                     /locus_tag="ACHJZ2_004722"
     CDS             complement(2946..3218)
                     /locus_tag="ACHJZ2_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4570681.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            complement(3259..3438)
                     /locus_tag="ACHJZ2_004723"
     CDS             complement(3259..3438)
                     /locus_tag="ACHJZ2_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4570682.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDVWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            complement(3574..5511)
                     /locus_tag="ACHJZ2_004724"
     CDS             complement(3574..5511)
                     /locus_tag="ACHJZ2_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4570683.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLTD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSSSGAEATGVTTLLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAAN
                     QYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGT
                     FGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDITRN
                     ATYPVLIDSIAVEVNSTDTAA"
BASE COUNT         1337 a         1653 c         1290 g         1417 t
ORIGIN      
        1 gttaactgcc ctgaaacggg aattttttct ctgcgtgaga gggggggcgg tgtccggagc
       61 gatcgttttt tacgccgaat gatacccccc cggtcgggtt acagtccgat gatgtcgtcc
      121 gctctgccac tacctccgga cacctccggc agcgtcgggt ccggcatacc acccgccgct
      181 tcacgagcag acttttgtcg atggcattcg gtacagagcg tccagagatt cgtctcctca
      241 ttaccaccac cgaactgaag tgcaattcgg tgatcgagtt cactgtcaca gaggtcaacc
      301 acacgaccac agagacagca ctgcccggca tccctcagcc agatacgacg cttgagggaa
      361 acccgggcac tgccactgac ccgacgctgt tcacccttca ggacattcac ccgccgggta
      421 ttcagagttt tgattctgcc cggtaacgta cgaagcacag ccatgtaaaa tcctcgccat
      481 atagcttgtc accagaggaa agaaaatgtc atcgaaaaac cggccccgca gaacaacaac
      541 ccgcaacatc cgatttccaa accagatgat tgaacaaatt aacatcgctc ttgaccagaa
      601 aggttcagaa aatttttctg cgtgggtcat tgaatcttgc cgccgggagc tggcagcaga
      661 cataaaatat gcccgtcagt tgactataaa aaagaatgat acacagtatg ctctgcgatg
      721 gctgttcata taactatttc tttatattgc tgaatttata aaaaatcaca gacattagct
      781 gtattaattc cgaattgaaa taatcagcca tatagaataa aaataaagca taacaataat
      841 aatcttctac ccaatcagta cattactgct gtgactccaa cacggcagtt tttttattga
      901 acagattcca gtttcttcca ccatcgcacc ggatgggcga ccatgagggg agaacgccgc
      961 gctccgttta cgcggtaaac cccggtgtgt atcgtttttg attatccccg cacactctcg
     1021 cagaggagtc tccctgtcgg gctgcggtct ctgttaatgc aggaatacgg cgacaatacc
     1081 gcgcatggat aataaggtcg ctcaacacac tggctgtaat tcagcggata ccattcggca
     1141 tttatcagta ttcatcacac actcaacggt gaattcttca tgcgtggcat tcacttcata
     1201 tgttcgtgaa taacattcag tgcatttacc tctgaacacc tcttcaagca gaacacggcc
     1261 atgttgcaaa acacggaacg gaattgttcc ctgaaaaggt tttaccgtta cctgtaattt
     1321 cttcatacat cctccggata ataaaaagcc tgcttagtac actgagtgcg gatatagtcc
     1381 tgtgcccctt ccacctgctt ctgcattgtc atcaaccgtt ctctgaggat gaaataatcc
     1441 cgttcagcgg tgtctgccag tcgggggccg gttgcattat ccacgccgga ggtgccggtg
     1501 gcttcacgca cggtaccggg gcaggtggcg ttgatccgca ggcgcttacg accagcggca
     1561 acatcagcgc gcagagtttc attttcagct ctcgcatcgg ctaattccct cgagtatttt
     1621 gcatcgagcg cagcaacatc gcgctggcgc acctgcatat cagtaatagt tgcgtttgcc
     1681 agctccagct ctctggcttt tttatcgcgc tgcgctttgt aggtgatggc gttatcgcgg
     1741 taatgattca gccccagact aagcgcacca caggccacca gcagggcaat gatgaccacg
     1801 cacagtacgc ggttcatttc accaccagcg tatctgaccg atgaaataac cggaggccat
     1861 aatcacaaac accagccaga taaggatgta cttccaggtt gataattttt cagccatcac
     1921 tcgaatctcc cgaatcagtt tgctaaaatc aaacacactt tctcctttga cttttccgga
     1981 gtcaggaaac acaaaacccc gcttgcagcc aacaaatggg gtttttactt ttattcactt
     2041 agtttttgtc agttcgcagg attttgtgtt atccgtccgt gtgagcaaac cgcatttttc
     2101 agcaaaatat tctgcttatc tgtcaattcc ccagcacgcc agcgcgctct cctggtcacg
     2161 ccgtgagacc tgaccataac agttatttga gcgaatacgg cagtctctgc caccgtcctt
     2221 aatccaccag cgaatcgctt cgcaggcacc ttttcgatct cctgcattaa ttcgtttata
     2281 aaacgtcgac gggaaacact tacctgggcc aatgttgtaa ggacagaatg acgcaatacc
     2341 cgctttctgg ggttcagtca gtggcacttt gatgtttttc tccacccatg ccagcgcctt
     2401 atcacgctca atggcgttaa cctggtcgca tttccccttc gacagcttca tgccaggaat
     2461 aacaggctta ccatccaccc gggtggctcc acggcagatg gtccagatac ccgcaccatc
     2521 acggtatgcc gtggtgtggt tgccttcctt ttcatccaga aactggtcga ggatttcagg
     2581 cgcagaagca cctgcggcaa tcagcgccag aacggcagcc gataaaccat agcggagttt
     2641 cctgctcatc agcttactct ccccgtgccg ccttacgcct gtcctctctg attttgaaat
     2701 acaggttcgt caggtacgtc agcagaccaa acagcagact ccccagcacg cctattgccg
     2761 cccactgaga cggggaaacc ctgtccagca actgcaggaa ccagtagccc gttcccaccg
     2821 ctgacgtggt gtatgacaca cctgttgtga ttttttccat ctggtacata ccccgtctcc
     2881 cgcaatccgg aagctcacaa catgaaaaag gccagcagct gtttactgat ggccctgact
     2941 ccccgttaca gcatcatgac cgattcgggt tgaggttcag tcgcatcggc gaccggtgat
     3001 tcaggctgaa cttcaccgct ctctgcggtg gtatctcccg cttcagtcgg tggctctgcc
     3061 tgtacaccaa gcagctcatc cagaatggca tcaacctctg catcaagacg cgcttccagg
     3121 ttatggcgaa gtttttgttt cagtgcgctc aggacttctt cagagcgcag gacttccttc
     3181 actgcctctg cagtgaccag ggatgtaatt tctgacatgg gattttctcg tcgaaaggtg
     3241 tgattaagaa agttgccgct aaatgagcgg ctcttcgggt ttgcttccgg ctgactgact
     3301 ggcgctgatt ttctcagcgg cccttttgtc aatctgtctg cgccagacgt cacgcatggc
     3361 cctgtaccca cccgaaagga gatacagcac acagaccacc gtacagaagt acagcattaa
     3421 ctggttcaga aatgtcataa tttctttccg ttattgttga caataagaac tgttttcatt
     3481 aaaaaaccag agtacgaaag tatcgttcct ttattttttc tccataggta tcaccaccgc
     3541 cagcgtccat tcctgtcgat ggcggttttt ttatcatgcc gcagtgtctg tgctgttcac
     3601 ttccaccgca atgctgtcta tcagtaccgg gtaagtcgca ttcctggtaa tgtctgtcac
     3661 atgcagttta tccgccgcaa atgcactgac cggtgactgc gtcagcgtga acggtgtgcc
     3721 atcctgacca tcaataaccg gcgtcacctg aaggctgtta ttcccggcaa agcggaaagc
     3781 cagcgtatgc cattcgttat caaatgcgcc aaaggttccc agtttcaggt tgtttgtcgc
     3841 cactttcgca ttgtggtaca tcacattcag gtcttttgca tctgtctgga tgtagaacgc
     3901 tgccagcagg ttattccccc cgtctccggt cagggcaacg ccctgtggca gtgaagagac
     3961 cggccagtaa aacgccataa catactggtt cgcagccagc gctcccgaaa ccttaaagcg
     4021 gcagcgaatc tgcccccctt tctgtaacag agccgcaccg ttgcccgcgg cgtactccag
     4081 cacccagctg cttttacctg cttccttggt cagcttcact gccttacctc cggttccctc
     4141 cgcatcgctg accacttctg ccctgccgcc actggctgac catccctgta ctttcaggct
     4201 tccctctgac tcgctggcaa ggtaagagag cagtgttgtg acgcctgtgg cttctgcacc
     4261 ggaagacgat gacgggcgca cctctgatac tgtcgatgat gcccccgcgt ttagcgccac
     4321 tcttcccgca tggcgcaaaa tcgccgttgc cagacggtcg gaaataatcc cgcggcgagc
     4381 ccatgaactg aaatggctcg ccctgtcctg tgacgtccag gtggctgagc tgtcacgcca
     4441 tttcgaaccg taatacccga tacccggaat gtccgggtct tcttccggtt tgttcgtcgg
     4501 cacattcacc ccgttctcat cggtcatgaa cggtacgaaa tggatattct tttccgtttt
     4561 gtttttgtag ctgccgtaca ccgtctggta cgaggattcg ttcttctgct tccagaaata
     4621 cgtcgtatct ccacagatcc agggaacacc gccagcagag ccaccaacgc actgacctgc
     4681 catatccgtc aggtctgcac ggaatttatc aaccagcgca ccaaactgtg ctgcgtgatt
     4741 tccgggcgtg ccgtcaaaat caaattcccc ctgcatccac accacggcaa acagcacatt
     4801 tttcgggttc ttcttcagtg ctgcttttgt tcgaccgata aggtccttat acagcggctt
     4861 gtccacaccc cagcgggttg aattctccga ggcaccactc gcgtcactgt atgtgccatc
     4921 agctccggtg gtgaacgctg aaccaccacg acagcacgga accagcagaa tgcccgcatt
     4981 cgccggtata aacggcagca gttttttggc gatatgcagc ccctgcccca cggtaccgta
     5041 ctgccctttt gacaggtccg ctttcggatg gttaagacgg ctcatgtcct gcacatcatg
     5101 cagacaatgg tccgccggaa tgatgtcgtt atatttgcat gctgcaccgc ccggtgtcac
     5161 cgtactgcgg cgcgccagct gcttaatacg cgggtccgga cggtcatatg tctccggcag
     5221 cggaagacct tcaccatacg acatgccatt tgactgccct gccagaacca caacaaagta
     5281 atactccggg tcgctggtgg cactgacagc caccgcctta ccctcattac cggtcaccgc
     5341 cactggtgtg gtgacatcac cttccgccgc aatggcctgc atcagggtat aaggcgtgat
     5401 ggccaccgga ctgccaaatg gctgccagcc ctccttcagt ttttgtgtca gtcgttcagc
     5461 aaggtctgac ggcgatgccg ccctgaccac atcgtagtgt ttaaatgtca taacctctcc
     5521 tgccgccagc aaagtaatta tccggaatac acaatcatgt aacacataaa aaacacgcta
     5581 taaaaaaacc atgtctctta tataacaaaa aaacgccgac aatttaaaat gtcctcttat
     5641 tttaaacaaa gaataaacga caccagaaaa acaactcagg ctgacagatt gctttca
//
LOCUS       DAZFBR010000198         5658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFBR010000198 DAZFBR010000000
VERSION     DAZFBR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.198"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            222..1154
                     /gene="rfaD"
                     /locus_tag="ACHJZ2_004725"
     CDS             222..1154
                     /gene="rfaD"
                     /locus_tag="ACHJZ2_004725"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO4570684.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1164..2210
                     /gene="rfaF"
                     /locus_tag="ACHJZ2_004726"
     CDS             1164..2210
                     /gene="rfaF"
                     /locus_tag="ACHJZ2_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO4570685.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2214..3206
                     /gene="rfaC"
                     /locus_tag="ACHJZ2_004727"
     CDS             2214..3206
                     /gene="rfaC"
                     /locus_tag="ACHJZ2_004727"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO4570686.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3203..4411
                     /gene="rfaL"
                     /locus_tag="ACHJZ2_004728"
     CDS             3203..4411
                     /gene="rfaL"
                     /locus_tag="ACHJZ2_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO4570687.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4447..5589)
                     /locus_tag="ACHJZ2_004729"
     CDS             complement(4447..5589)
                     /locus_tag="ACHJZ2_004729"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO4570688.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         1560 a         1245 c         1270 g         1583 t
ORIGIN      
        1 cttccgttat ttatcataca tcggaatatt gatactaaag cactattcac atgcaaaacc
       61 aacatccgcc atgaaggact agctaaaacc caaactagtt tgttgcaatt agcatccttg
      121 cacctctatg taaagggctg aagggattcg gatgtgatgg tatgattaca gacattcgtg
      181 tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc gttaccggcg
      241 gcgcgggctt tatcggcagc aacatcgtta aagccctgaa tgataaaggc atcaccgata
      301 ttctggtggt ggacaacctg aaagacggca ccaagtttgt gaacctggtg gatctgaata
      361 tcgcagacta tatggataag gaagacttcc tgatccagat tatggctggc gaagagttcg
      421 gcgatgtcga agcgattttc cacgaaggcg cgtgctcttc caccaccgag tgggacggca
      481 agtatatgat ggataacaac tatcaatact ccaaggagct gctgcactac tgcctggagc
      541 gtgaaatccc gttcctgtac gcttcttccg cagccaccta cggcggacgc acctccgact
      601 ttattgaatc ccgcgagtac gaaaaaccgt tgaacgtcta cggttactca aaattcctgt
      661 ttgatgaata tgttcgccaa atcctgccgg aagcgaactc gcagattgtt ggcttccgct
      721 atttcaacgt ttatggaccg cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc
      781 atctcaacac ccagcttaat aacggtgaat caccgaagct gttcgaaggt agcgagaact
      841 ttaaacgcga cttcgtctat gttggcgatg tggcagatgt aaacctgtgg ttcctggaaa
      901 atggcgtttc cggcatcttc aacctcggca ccggtcgtgc ggaatccttc caggctgtag
      961 ctgatgctac gctggcttat cacaagaaag gccagatcga atacattccg ttcccggata
     1021 agctgaaagg ccgctaccag gcgttcactc aggcagatct gacaaatctg cgcgcggcgg
     1081 gttacgacaa accgttcaaa accgttgctg aaggtgtaac ggaatacatg gcctggctga
     1141 atcgcgacgc ataagagctc tgcatgaaaa tactggtgat cggcccgtct tgggttggcg
     1201 acatgatgat gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc caggcgataa
     1261 tcgatgtgat ggcaccggca tggtgccgtc cattattatc gcggatgccg gaagttaacg
     1321 aagctattcc tatgcctctc ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg
     1381 gtcatagtct gcgtgaaaag cgctacgacc gcgcctacgt cttacctaac tccttcaaat
     1441 ccgcattagt gcctttcttc gcgggtattc ctcatcgcac cggctggcgc ggcgagatgc
     1501 gctacggttt actcaacgat gtacgcgtgc tcgataaaga agcctggccg ctaatggtgg
     1561 aacgctatgt cgcgctggcc tatgacaaag gcattatgcg cacagcacaa gatctgccgc
     1621 agccattgtt atggccgcag ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc
     1681 aattttcgct ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg gagtttggtc
     1741 cggcaaaacg ctggccacac taccactatg cggagctggc aaagcagctg attgatgaag
     1801 gttatcaggt ggttctgttt ggctctgcga aagatcatga agcaggcaat gagattcttg
     1861 ccgctttgaa taccgagcag caggcatggt gtcggaacct ggcgggggaa acacagcttg
     1921 atcaagcggt tatcctgatt gcagcctgta aagccattgt cactaacgat tctggcctga
     1981 tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct gtatggtccg agtagcccgg
     2041 acttcacacc gccgctatcc cataaagcgc gcgtgatccg cctgattacc ggctatcaca
     2101 aagtgcgtaa aggtgacgct gcggagggtt atcaccagag cttgatcgac attactcccc
     2161 agcgcgtact gaaagaactc aacgcgctat tgttacaaga ggaagcctga cggatgcggg
     2221 ttttgatcgt taaaacatcg tcgatgggcg atgttctcca tacgttgccc gcactcactg
     2281 atgcccagca ggcaatccca gaaattaagt ttgactgggt ggtggaagaa gggttcgcac
     2341 agattccttc ctggcacgct gccgttgagc gagttattcc tgtggcaata cgtcgctggc
     2401 gtaaagcctg gttctcggcc cctataaaag ctgaacgcaa agcgtttcgt gaagcgctac
     2461 aagcagagaa ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc gcggcgctgg
     2521 ttacgcgtct ggcacatggc gtaaagcatg gcatggactg gcaaaccgct cgcgaacctt
     2581 tagccagcct gttttacaat cgtaagcatc atattgcaaa acagcagcac gccgtagaac
     2641 gcacccgcga actgtttgcc aaaagtttgg gctatagcaa accgcaaacc cagggcgatt
     2701 atgctatcgc acagcatttt ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat
     2761 ttcttcatgc gacgacccgt gatgataaac actggccgga agaacactgg cgagaattga
     2821 ttggtttact ggctgattca ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg
     2881 aagaacgggc gaaacgactg gcggaaggat ttgcttatgt tgaagtattg ccgaagatga
     2941 gtctggaagg cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg
     3001 ggttaagcca tttaacggcg gcactggata gacccaatat cacggtttat ggaccaaccg
     3061 atccgggatt aattggtggg tatggggaga atcaagtaga gtgtcgttct acaagtatgt
     3121 ctcttgcaga tttgccagct caaaccgttt ttcaaaattt aaatcttgaa ataataacca
     3181 ataagttgac atcggagata agatgacctc aacattattt ttctctctcg agaaaaaaaa
     3241 ctggatagcg tactggaaca gagctctcgt attcttattc attaccacct attttttggg
     3301 tgggattaca aggtataaac atcttattgt tattcttatg acaataacga caatcgtcta
     3361 tctctgcaaa cggccaaaac actatctctc actatttaaa acatttcttt ttggtagtgt
     3421 tgccatatta actattgctg cattgctgtc acttcttcaa tcccctgatg caggtgctag
     3481 catgaaggaa attttcaaag ctattattga gaatacttta ctatgcacaa tagcaatacc
     3541 gatcatattg agagacgaga aaagagaaga tgtcgaaaaa atcgttttct tctcatttat
     3601 tagtgcgttg ggcttacgct gtttttctga attgattacc tattataagg actatcaaca
     3661 agggataatg ccattcgcag attatagaca ccgtagcatt tctgactcga tggtcttttt
     3721 attccctgca ttgttaaatc tctggcttat caaatcagca aaataccgca tttcttttgt
     3781 ggttctaagc gttattttta tttttctgat attaggaact ttatccagag gggcctggct
     3841 ttccgtgtta gtcattggat taatatggat tctgatgttt aaacaatgga agttactatt
     3901 agtaggagta atggttgcca tcattgcatt gtcggttatt ttcacacata aggagatgac
     3961 cgcaaagcta acgtataaac ttcaacaaac taatagttct tatcgctatg caaatggtac
     4021 tcaaggcagc gcactcgatc taatattaga aaatcctgtt attggttatg gttacggtaa
     4081 cgttgcatat aaagatgtct ataataaacg tgtcattgat tatccagaat ggacctttag
     4141 acaatcaata gggccacata attttgcgct attcatctgg tttggcactg gtttattagg
     4201 gctggtaagt cttatgatgc tatactgtgc aatattgaaa gagtgtataa aaaatggcgt
     4261 caagaataaa tatcgctcac catataatgc atattatata atcttactat cttttatagg
     4321 ttattttgtt atccgtggaa acgtagaaca aattgaacca aatttattag gcgtttacgc
     4381 cggcttatta ttagcgatga aaaacaagta aaaaataaaa aaggctgcat aatgcggcct
     4441 ttttatttat tcaaaccaat tatgaataac ctcttcgaat ctttgagtta cgccatccca
     4501 actgtagtga tcaaacacaa agtcctgacc ttgctttgca actgctgtta attcaggatt
     4561 cgctaacgtt tttaaaatat cgctgcttat ggagtctgca gtcataggtt cttttaaatg
     4621 gaatcccgta gtattttctt tgacaaattc cgtcatcccc ccacgcgtac tcactaaaac
     4681 aggcttacca gcacccatag cttcaattgc caccatgcaa aatggttcct ggaactggga
     4741 aggaatgacg actaaatctg ccagtgggta ataacaatgc atcttttcag gcggtatgct
     4801 tcctaacata atgcaacgat ccttcaaacg ttttgcaatt tccctgacat tgcgttgata
     4861 tgcacctttg tcacttttac tcatttctgt ataatcacca atcacaacta attttaaatt
     4921 tttgtgagca gcggccaatt tttcgaatga ttgcatcaac agcaatatgc ctttgtcagg
     4981 gacgatacgc ccagcataaa aaataatttt ctcttcaggg gagatattaa gttcgcttct
     5041 agtgatcggc tgaaaattag attgataggt ttctaaatca attccatttg gtacaatctc
     5101 aatatctgca tttgctaaat aagattggta atactttttc aagtataaac taggcactat
     5161 cattttaaca ttttgctcaa gcaattttgg ttcaaatgca ttatgcatat gtataaccac
     5221 tcttgcctga ggagctcgct tacgaatttg agtgtatagc ttcatgctat tatgaactac
     5281 aataacactt tctttcgtta taggaaaatc atgtgcaata ttcaatattc tctgtgaata
     5341 tggaagagga tcaagtcttg tccatttttg aaataaccgc ttatatatgc gactgaatcc
     5401 aaccctatga atactacaat tatcattaac aaaggtgtaa tttgagtatc ctggattttt
     5461 gatacaaaca atacgatttg gaaaattggt acgctgagca acctgataca tccacgtttc
     5521 aacagcagcc gctccgcgag gaggaattga aaaaatagga gtaaccgtaa atattatttt
     5581 atcaaccatc tcgtatatta acctttcata acattatatt taattgctgt aaaaacacca
     5641 tcgagaaatt ttttctgg
//
LOCUS       DAZFBR010000199         5462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFBR010000199 DAZFBR010000000
VERSION     DAZFBR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.199"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            87..371
                     /locus_tag="ACHJZ2_004730"
     CDS             87..371
                     /locus_tag="ACHJZ2_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO4570689.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENGYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKSSRFRG"
     gene            380..1738
                     /locus_tag="ACHJZ2_004731"
     CDS             380..1738
                     /locus_tag="ACHJZ2_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFO4570690.1"
                     /translation="MQKRVLPIDVYSDETDEPRLIVRKKEHDLYKYYILQDSKDYKPE
                     KPSDTELVFISNAETIRDYLLRLSVDELKLLAKYILQNVYIVFVQTDDFASSFRLFNV
                     LNSRGLPLSNADLLKNALFESASTHNKKSEQIESAWSQIEDMVGVRRLDKFLTLHKLS
                     EKKDRDRVLQKGFEAFIENLQQQFDGDAIAMSLMLVNSAKNYTKILENDFEHPSIRRK
                     IASLSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFITAFEKVYMHGWLKKQIKSQR
                     EMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALDEDLYEPRPNQVNLIKAILLR
                     LDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYWLHKIGNLTLISGS
                     KNSEAQHYDFIKKKSIYEKLNSKSSFDLTKDVCNSSEWGLAELKMRHEKMKTQLKKLW
                     LV"
     gene            complement(1807..2763)
                     /gene="yjiA"
                     /locus_tag="ACHJZ2_004732"
     CDS             complement(1807..2763)
                     /gene="yjiA"
                     /locus_tag="ACHJZ2_004732"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO4570691.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(2774..2977)
                     /locus_tag="ACHJZ2_004733"
     CDS             complement(2774..2977)
                     /locus_tag="ACHJZ2_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO4570692.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(3027..5177)
                     /gene="btsT"
                     /locus_tag="ACHJZ2_004734"
     CDS             complement(3027..5177)
                     /gene="btsT"
                     /locus_tag="ACHJZ2_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO4570693.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
BASE COUNT         1521 a         1241 c         1343 g         1357 t
ORIGIN      
        1 aacaaaatat ataaaaatct tatagttgcg tttctttcga gcaagaaaga taatgttatt
       61 gttgattgtt tgattgggta tatgaaatgg aagctaaaga atgtaaggtg caggatatcc
      121 ttactgaaaa caaaaagttt attattcctt cttatcagcg tccttacagt tggacggtag
      181 ataatgcaga gcaactcatt gatgacattt ataaaagctc tcaatcagaa gagaatggat
      241 attttattgg gagtatgatt tgtatcaata aagggcaaaa tcagtatgaa gttgtagatg
      301 gacaacaacg tcttacaacg ttaagcatta ttgtctccga gctaaaaaaa tcatcccgat
      361 tcaggggata aaagatgatt tgcaaaaaag agttctgcct attgatgttt attctgatga
      421 aacagatgaa ccaagactaa ttgttagaaa aaaagagcat gatctttata aatattatat
      481 actgcaggat tctaaagatt ataaacctga aaagccatcg gatactgagt tagtattcat
      541 tagcaacgct gaaacaatta gagattattt gttgcggtta tctgttgatg agttgaagtt
      601 gcttgctaaa tatattttgc aaaatgtata tatcgtattt gtgcaaacag atgatttcgc
      661 atcatcattt agactcttta atgtattaaa tagtcgaggt ttacctttaa gtaatgcaga
      721 tcttttaaaa aatgcactgt ttgaatcagc ttctacacat aataaaaaat cagagcaaat
      781 agaatcagca tggtcgcaaa ttgaagatat ggtcggagtg agacggcttg ataaatttct
      841 tactcttcat aagctttccg agaaaaaaga tcgtgacagg gtgttacaaa aaggttttga
      901 agcttttatt gaaaatctcc agcagcaatt tgatggagat gctattgcta tgtcactgat
      961 gttggttaat tctgcaaaaa attatactaa gattttagag aatgattttg aacacccttc
     1021 aatacgacga aaaatagcat cgctaagtaa tttaggggta gatgagtgga ttcctccggt
     1081 tatggcattc atgaatcgaa tggctcgtac agaagatttt aatttagatg atttttctca
     1141 atttattacc gcctttgaga aggtatatat gcatgggtgg ttaaaaaaac aaattaaaag
     1201 ccaaagagaa atggtttgtt attcagctct ggtagcaata aataatgata tgccttttga
     1261 ttctgtgata aatcagataa accaacatgc agataattca ggttttatag ctgctctgga
     1321 tgaagattta tatgagcctc gtccgaatca agtgaactta ataaaagcaa ttttgcttcg
     1381 cttagatatg gagcaacaag atgagagcgt gattaaaacc tatacgggga gaatcacaat
     1441 tgagcatatt cttcctcaag cattagttaa tgaatactgg attaatagat tccaacctca
     1501 agagcatgtc tactggctgc ataaaatagg taatttaaca ctcataagtg gttccaaaaa
     1561 ttctgaagca caacactatg attttatcaa aaagaaatct atttatgaga aacttaatag
     1621 taaaagctct tttgatttaa caaaagatgt ttgtaattct tcagaatggg gattggctga
     1681 actgaaaatg agacatgaaa aaatgaaaac gcaattgaag aagctttggt tggtctgaca
     1741 tatctaaaga gttccactcg taataatgtt gcggtggaac tctcaaaaag ttgatctttc
     1801 ccgaacttac ttcctcaacc ccgcaaacgc tgcccgaatc tcttcctccg gcaactgaat
     1861 cccgataaaa accattgtgc tatgcggttt ttcatcgccc cacgggcggt cccagtcggc
     1921 gctgtagagg cgctggacgc cctggaacag caggcggtta ggttcgccgt caatccacag
     1981 catccctttg taacgcagca gtttatccgc cgattccagc agcaggtttt ccatcacgcg
     2041 ggaaacttcg ctgatatcta ccgggtaatc cagttctacc acaatcgacg aaatatcgtt
     2101 ttgtttatcc gcgataaagt ggaaacgcgg tttggtgctg acgacgtttt cttccagcat
     2161 aaaaccgttg gtgttgaaca gcaggcccag gtcgatgtcg ccgtgggtaa cggtgtagac
     2221 cggtgcacgg gcgttgatgc gcgccaggcg ttcgcgcagt ttttctgctt cgcctgcgac
     2281 gtcggttttg gtcagcagaa tacggtcggc gtagccaacc tgggactggg cgatggtgaa
     2341 ctggttcatc tgctcatcgg catgtaccgc atccaccagc gcaatcacgc cgtccagcag
     2401 gtaacgctgg cataaaatct catgggagaa aaaggtctga ataatcgggc cgggatcggc
     2461 catgccggtg cattcaatga ccagacgatc gaactgaata ttgcccttat cgagattatc
     2521 cagcaggtcg agtagtgcgt cttccagctc gttggagcgc gaacagcaga tgcagccgtt
     2581 ggtcagcgtt ttgatctgcg tggcgcggtc gccaatcagt tgatcatcaa cggagacttc
     2641 gccaaattcg ttttcaatca cggcaatctt gtagccgtgt tgttcgttaa gaatatggcg
     2701 cagcagggtg gttttgcccg cgcctaaaaa accggtgagt agggtaactg caatcgggtt
     2761 catgctctct ccattaacaa cagcgcatac cgcctttacc atcgccgccg tagcgcgcgt
     2821 tctggcgttc gcggaagaat tcttcatagc tcatgtacgg cttgtcggga tggttggtct
     2881 tcatatgctc gacgtagttg tcgtagtctg gaatgccaat caacatcttc gccgcctggc
     2941 cgagatattt ttttgcctgt cctaagttac caaacatagt tcactctgat aagaacaaag
     3001 ccccgccgaa gcggggctaa acacggttag tggtgcgaag agatcttcac gccgccttcc
     3061 gggattggaa cgtacggtgt ttctttgtca gtacgtttgt cgctgttacg taccgcaagc
     3121 caggttttga aaccgtagaa gatgatgctg tacaccacaa tcaggaacag aatactcagg
     3181 cctgcgttgg tgtagttgtt cacaacgatg tggttcatgt tggcaatctg ctgcgccgta
     3241 aggtcagtac cgttagcaat cttctctttg tactggcttg ccatgtagaa gaagccttcc
     3301 atctgcgggt tggtgctgaa cagtttcaga cccagcgccc aggtggtgca gataagcagc
     3361 catacagccg gaacaacagt tacccagatg tattgggtgc gcttcatctt aatcagcaca
     3421 acggtgccca gtaccagcgc tacggctgcc agcatctggt tggagatacc gaacagcggc
     3481 cacaggctct taacgccgcc cagcggatcg accacgccct gatacagcag gtagccccac
     3541 agacccacac agcccgcagt accgatgata ccagcaacca gagagtcggt ttttttcagg
     3601 aacgggatga agttacccag caggtcttgc agcataaagc ggccagaacg ggtacccgca
     3661 tccagcgcgg tcaggatgaa cagggcttcg aacagaatac cgaagtgata ccagaagccc
     3721 atgtcagcca tcggcagcac tttgtggaac acatgagcga tacctaccgc cagcgtcggc
     3781 gcaccacctg cacggttcag gacagaaggc tcaccaatgt ctttcgcggt ttgcaggatc
     3841 tgctctggcg aaatcacgaa gccccaagag ctgacggtcg ctgccgcgtg tgcggtaacg
     3901 tctttcagct gcgccatgat gatcggcgcg ttctcgccac ccatttcatg caggttaggc
     3961 atggtgatgc caaggcccgc gggcggggtg ttcatcgcga agtaaagacc cggttcgatg
     4021 atggatgcag caaccagcgc cataatcgcc acgaaggact ccatcagcat tgcgccgtag
     4081 ccgatgaaac gcgcgtcggt ttcgttagcc agcagtttcg gcgtcgtacc ggaagagatc
     4141 agcgcgtgga agccagatac cgcaccacag gcgatggtga tgaacaggaa cgggaacaga
     4201 gcgcctttcc acagcgggcc agtaccgtca atgtactggg tcatcgcagg cattttcagt
     4261 tccgggttca gcaccacgat acccagcgcc aggccgacga taacgccgat cttcaggaag
     4321 gttgccagat agtcgcgtgg tgcgaggatc agccacaccg gcagcagcgc ggaaacaaac
     4381 gcatagccaa tcagcgcgaa ggtaatggtg gtgtctttaa aggtcagtgc cggaccccag
     4441 tacggatcgt gagcaatcac gccaccgaag tagatagagg caaccagcag cacgatacca
     4501 ataacagaga cttcacccac acgccccgga cggataaagc gcatgtagat acccataaac
     4561 agcgcaattg gtacggttga gcaaacggtg aagacacccc acggactttc tgccagggct
     4621 ttaaccacaa tcagcgccag gacggcgagg atgatgatca tgattaagaa acagccaaac
     4681 agcgcgatag tccccggtac tggccccatc tcttctttga tcatctcacc gagagatgcg
     4741 ccgttacggc gagaggagat aaacagcacc ataaagtcct gaaccgcacc ggccagcact
     4801 acccccgcca gcagccacag cgtgccaggc aggtagccca tctgcgcggc gagaaccggt
     4861 cccaccagcg gaccagcacc ggcgatagcg gcgaagtggt gaccaaacaa cacgtaacgg
     4921 ttggtcggaa cgtagttcag accgtcgttg ttaataaccg caggcgtcgc gcgcgtgggg
     4981 tcgagtttca tcaccttctg ggcgatgtac agactgtagt agcgatacgc caccagatac
     5041 accgacacag aggcgaccac gatccacagg gcgctgacgt gctccccccg acgtaatgca
     5101 actaccgcga ggcagaatgc accgatgatt ccgagaatca cccagggtat gtgcttgaat
     5161 agttttttcg tatccatagt aaaacctggc atgtattgat taatagttgg ccgaagccgt
     5221 tctaggtttg cgttgcgttt gaggaggtaa attgaccgct atcctgcaat gatgctgcca
     5281 gagttacgcg cgcgtaaagt aaggtaaata actgagtggt tattttaggg acgtaagcgg
     5341 tcagttttgc ggttgagcgg ttttgagcaa ggtttatgaa ggttatttat gtgatgatga
     5401 tcacaaccga agcgattttt agccctcggt tgtgtaactc atttaaaaat tatattcata
     5461 ct
//
LOCUS       DAZFBR010000200         5458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFBR010000200 DAZFBR010000000
VERSION     DAZFBR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.200"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..303)
                     /locus_tag="ACHJZ2_004735"
     CDS             complement(<1..303)
                     /locus_tag="ACHJZ2_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570694.1"
                     /translation="MSQPLSEILTWDDEQWEVFVHDWLIVCKSDDYPWSERLGGAGDK
                     GRDVVGYKSDPNVEGYSWDNYQCKLYKKSLGFSDVVVEFGKLIYFTLNGDYPIPQKY"
     gene            complement(307..1488)
                     /locus_tag="ACHJZ2_004736"
     CDS             complement(307..1488)
                     /locus_tag="ACHJZ2_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4570695.1"
                     /translation="MPLNDMQIRRAKPEAKAYTFGDGLGLSLLIEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLAMQTESENAFEK
                     IAREWHQLKSAKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCSEVFRYAIATGRAEYNPAADLSSALEVHQSNHFPFLKADEIPDFLRA
                     LEGYSGSKLVQIATKLLMITGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVP
                     LSSQAVDLLNELKIMTGNYRYVFPGRNDPNRPMSEASINQAIKRIGYGGKVTGHGFRH
                     TLSTILHEQGFESAWIEIQLAHVDKNSIRGTYNHAQYFSGRKSMMDWYSNLIFERLKR
                     S"
     gene            1975..2409
                     /locus_tag="ACHJZ2_004737"
                     /pseudo
     CDS             1975..2409
                     /locus_tag="ACHJZ2_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2451..3194)
                     /locus_tag="ACHJZ2_004738"
     CDS             complement(2451..3194)
                     /locus_tag="ACHJZ2_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4570696.1"
                     /translation="MATTCSVILILESFDVYFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLKANNLRDISDCREYPSYLKINFADFSLIKGLISWANHCAE
                     YIEIFDESIAFTCLSAFSSEKQFGVFLFGCLKSTGAKVKTIIHTDLSAPWRLKDISSR
                     LYLSESLLKRKLKEEGVSFSKIILDERMQMAEYLLSTRCYPISKVAKVCGYASVSYFT
                     YVFRRYFGVSPSQYSQRSSESKILTHQGI"
     gene            4019..4792
                     /locus_tag="ACHJZ2_004739"
     CDS             4019..4792
                     /locus_tag="ACHJZ2_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570697.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAATDDDELLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASQAEVILATVRGIVRGVFIADKWLKSTRENFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(4853..5407)
                     /gene="pinE"
                     /locus_tag="ACHJZ2_004740"
     CDS             complement(4853..5407)
                     /gene="pinE"
                     /locus_tag="ACHJZ2_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HFO4570698.1"
                     /translation="MLIGYVRVSTNNQNTELQRNALECAGCELIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVVLVEELRERGINFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIASGVP
                     RQKVAIIYDVGISTLYKKFPVGDK"
BASE COUNT         1642 a         1262 c         1084 g         1470 t
ORIGIN      
        1 gtacttctga gggatgggat aatcaccatt cagagtaaaa tagataagtt ttccaaactc
       61 aactacaaca tcagagaacc ctaaactttt tttgtacagt ttgcattgat aattatccca
      121 agaataacct tctacgttag gatccgattt atatccaaca acgtctctac ctttatctcc
      181 agctcctccc aaacgttcgc tccacgggta atcatctgat ttacagacaa taagccaatc
      241 atggacaaat acctcccatt gttcatcatc ccatgtcaat atttccgaaa gtggttgaga
      301 cacaaattaa ctccttttta gtctttcaaa tatcaaatta ctgtaccagt ccatcataga
      361 cttccttcca ctaaaatatt gagcatggtt ataagtcccc ctaatagaat ttttatctac
      421 atgagccaac tggatttcaa tccaagcact ctcaaaacct tgctcatgca ggattgtaga
      481 aagggtatga cgaaaaccat gtccagtgac ttttcctcca tacccaatac gcttaatggc
      541 ttgatttata ctcgcttcgc tcattggcct attcggatcg ttccgccctg gaaaaacata
      601 acgatagttc cctgtcatga tcttgagttc attgagtaaa tctaccgctt gagatgataa
      661 gggcacaaga tgtggcctac gcattttcat cctttcagca ggaatttccc aaatagcgtt
      721 atccagatca aattcttgcc ataatgccgc gcgtaattcg atggttctca cacccgtaat
      781 catcagtaat ttcgtggcta tctggacaag cttactcccg gagtaaccct ctaaggcacg
      841 tagaaaatca ggtatctcat cagcttttag gaatgggaaa tgattggatt ggtgtacttc
      901 gagagcgctg gagagatcag ccgcaggatt gtactccgcc ctacccgttg caattgcgta
      961 gcgaaacact tcggagcaac gctgccgcac tttgcgcatt ttctccaacg caccacgttt
     1021 ctcaatttta cgcagaacgt tcagcagctc tagcggttta atctctccca caggccttgt
     1081 tccgacataa ggaaaaatgt cgttcttaaa cgcttccatg atgtctgatg catatcccgc
     1141 cgaccattta gcagatttaa gttgatgcca ctctctggct atcttttcga aggcgttctc
     1201 tgactctgtt tgcatagcta gcttttgctc ttttcgaacc tcactagggt tctttccttc
     1261 tgccacaagt tttcgagctt catcacgacg ggaacgagca tcggcaaggg tgatcgttgg
     1321 gtaaacacca agcgagatca ttttgggttt gccggcatag cgatagcgga accgccaact
     1381 cttgcttcca ttaggttcta taagtaatga caaccctagc ccatctccaa atgtataggc
     1441 tttagcttca ggcttagcgc ggcgaatctg catatcgttt aaaggcatgt gtataggaat
     1501 ccagaccgaa caggaacata tacacaatcc tatacacatt cagtaccgga ttctactgga
     1561 tggttacgga ctactatgga cttaaaagaa taaaaaatgt tataaatcag agattttatg
     1621 gactaatacg gacgtttgag gaagttgaga tggtgccgat aataggagtc gaacctacga
     1681 ccttcgcatt acgaattata agaatccgct tctaattcaa agcattaccc catcaacact
     1741 gcgctcacac gtcccaccac atcaaaacat gtaaagcctt gcaagccatt gcgaggcctt
     1801 atgtgtctca gttttgtccc accttgtatt acgacttgca tagccaatga agataaacgt
     1861 gacgacaaac ggcgcagcag tcttcttttc tttcacgact tctccagcat gcataccttt
     1921 ctgccatgac tgaagtgaat gcctgttatg agcgaggatt ggcccagatg aatcgccctg
     1981 gactgttgcg atcgtgaagc catagactgg gccgcaagca cgggaggtta cgataaagcg
     2041 acagtgcagg atgtcatgtt aggagcgata gaaaaacgct ttggcgataa agtaccggaa
     2101 cagtccatcc agtggctgac agataacggt tcagcgtata gagcgcatga aacacggcag
     2161 ttcgccagag agttgaatct ggagccctgc acaacggcaa tcagcagtcc tcagagtaat
     2221 ggaattgcag aaaggttcgt gaaaacgatg aaggaagact atatcgcgtt catgccgaaa
     2281 ccgaatgtaa gaacggcatt gcataatctt gcagtggcga tcgaacatta caatgaaaac
     2341 catccgcaca gtgcgttggg ttatcgctct ccgcgagaat atcgacgtca gcgggtaacg
     2401 ttaacttaag atacaccccc tgtctggaaa taagggggca agaacaatga tcagattccc
     2461 tggtgagtaa gaattttact ttctgaactc ctctgagagt attgacttgg agaaacacca
     2521 aaataacgtc taaatacata agtaaagtat gagacactgg cataaccaca gaccttagct
     2581 actttactaa taggataaca acgagtgctg agtaaatatt cagccatttg catcctctca
     2641 tcaagtatga tcttactgaa tgataccccc tcttctttca atttcctctt tagtaaactt
     2701 tcgctgagat ataatcttga tgatatatcc ttaagacgcc atggtgcaga taaatccgta
     2761 tgaataatcg ttttaacttt agcccctgtg cttttcaaac atccaaacag aaatactcca
     2821 aattgttttt cagaagaaaa tgcagagaga catgtaaaag caatagactc atcaaaaatt
     2881 tctatgtatt cagcacagtg attagcccaa ctaattaatc ctttaattaa actgaagtca
     2941 gcaaagttta tttttaaata agatggatat tccctacaat cagaaatatc ccttaagtta
     3001 ttagctttca aatagcgact aataaactca gcgccaaaat ccgccactat cactctttca
     3061 ggatatgtca ggaaaaaatc tctagagcta gagtcgacaa gtacagatga acctctctcc
     3121 agaaacacac tctcttttcc gaaataaaca tcaaaggact ccaaaatcaa tataactgaa
     3181 catgtagttg ccatatcatc cacccaattt aactgaaacc aggatgaagt atatgtatat
     3241 aaagttcact ttgccaaact tcagtataaa aaaccactaa aaatagggtg tgataaaaaa
     3301 taccgtaaca ataaagcaaa ggattataaa ttccgttaca gttacaaatg atactcaaga
     3361 aacaattcct taggaaaaac ttatttacag ccaataagta agacacttat atgatatcaa
     3421 gttttcataa aacataacta ggctaaatag ctgcgcctaa taccgctaca cttttgccag
     3481 cccatgtttg cctccgggta ttgacccctt ctctacgcaa cttcagttcc caccaccaac
     3541 tttgcggcag ctttgtagga tcaatgtcta aaagaataat ggtgaccgat aagaaaacga
     3601 ctgaataact gcagattttc gctcgaaacc ttcctgtcag atccatagcg aatcaagtgc
     3661 tgaatgtcac agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg
     3721 attgttctgt ttaggaaaag caaggcggga agtcgggaga taagtcattg ataaagtggc
     3781 ggagagaggg ggatttgccc ccccggtaga gttgccccta ctccggtttt cgagaccggt
     3841 ccaatcatca aacgaaacat aaaattaatt cacattatga ggaaaagtat cttttttgta
     3901 ctatgtaaat tcaaagactt agcctcattt ccccgatgat tttctcaaca ctactggttg
     3961 tgagcccttg caatgttcat taatatacgt ctcacgaata attcatagat attgcaaaat
     4021 ggatattact gagtttcctt ctggagtaat tgaacacctt ggctggtatg tataccgatt
     4081 gattgatccg agggacggaa gcaccttcta tgtagggaaa ggcaaaggta accgcgtatt
     4141 tgcccatatg cgcggtgaag tggcagcgac tgatgatgac gagttactga gcaacaagct
     4201 aaagcaaatt agagaaataa ggttagcagg acttgaggtt atccatgtca tccatcgaca
     4261 cggaatgact gatgaaaaga cggcgtacga agtcgaagca gcacttattg atgcctaccc
     4321 tgggttaacg aatatcatga atggtgctgg cagcaatgaa ttcggcgccg cgcatgtcaa
     4381 agagttgata gcaacatatc aacccgaaac cataacattt catcataaag cattaatgat
     4441 ttccgttaac agaagtgcaa aggattcaga gctttatgat gcggttcgat ttagctggcg
     4501 cattaatgtc tctcgcgcca gccaagcaga agtcattctt gctactgtaa gggggatcgt
     4561 tcgaggggtt ttcattgctg ataaatggct caaatcaaca cgtgaaaatt tccctacgat
     4621 gaaatactgg gacgaggatc cggactttga ggcaacacaa agttctcgct atggttttga
     4681 aggtcgagaa gccccacctg aaatagcaaa tctttatctt ggaaaaaaaa taccagatga
     4741 attaagaaaa aaaggagcta tgtccccggt ccgttactca cctaattttt gagtctttaa
     4801 gtgataagca taaaccgcag cacgatcttc ttgcatacga cgtgctacgg tttcatttat
     4861 ctccgaccgg aaacttctta tacagtgtcg atataccaac atcatagatg atcgccacct
     4921 tttggcgagg aacgcctgat gcaattaatc gcccggcctg cgcccattgt tctggtgtaa
     4981 gtttgggacg acgtccacca attcgtccct gtgcgcgagc tgcttccagt ccagcttttg
     5041 ttcgttcaac aatcagttca cgctccattt cagccagggc acccatcaca tgaaagaaaa
     5101 agcgccccat tggggtactg gtatcaattg aatccgtcag actacgaaag ttgatgcctc
     5161 gttcgcgcaa ctcctccacc agcacgacaa gatgccgcat actgcgcccc agtcggtcca
     5221 gtttccagac caccagcgtg tcacctgccg ataatgtcct gagcagcttt ttcagtcctg
     5281 gtctgtcgga ctttgtaccg cttatcttgt cttcaaaaat cagctcacat cctgcacact
     5341 ccagcgcatt acgctgtaat tccgtattct ggttatttgt tgatacgcgg acatagccaa
     5401 taagcatgag cattcccctg agtaaaaacc ggggatgatg ccagttagcc ataatctc
//
LOCUS       DAZFBR010000201         5430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFBR010000201 DAZFBR010000000
VERSION     DAZFBR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.201"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..585
                     /locus_tag="ACHJZ2_004741"
     CDS             <1..585
                     /locus_tag="ACHJZ2_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4570699.1"
                     /translation="DELLSTLSYDSETGIFKWLKTNSVVRVKGSIAGGVSGGYICISI
                     NNVLYKAHRLAWFYVYKKWPPKFIDHVNGNRLDNRISNLRLATEEQNARNIVGNRLNT
                     SGAIGVSWYKPTGRWKSYVGYKNKTISLGYFDSKEDAAFIAALARKKLYGTYASKALN
                     CEHELLSQFNNDEDKLAEYLKEKYKRTRKRVKKR"
     gene            1049..1381
                     /locus_tag="ACHJZ2_004742"
     CDS             1049..1381
                     /locus_tag="ACHJZ2_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570700.1"
                     /translation="MDESRKQFEEYVAKKLRLPFEMITEARNGDRYFAFSSMDIRHSL
                     NDWWALWQASRAAIELDIDWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAA
                     GIKEKNFD"
     gene            1514..1729
                     /locus_tag="ACHJZ2_004743"
     CDS             1514..1729
                     /locus_tag="ACHJZ2_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570701.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDFK"
     gene            1740..1976
                     /locus_tag="ACHJZ2_004744"
     CDS             1740..1976
                     /locus_tag="ACHJZ2_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFO4570702.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RVAWAPSVAAATEKWNRRAGDEANLSASQRSNQK"
     gene            1933..2379
                     /locus_tag="ACHJZ2_004745"
     CDS             1933..2379
                     /locus_tag="ACHJZ2_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO4570703.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSAPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGKAA"
     gene            2376..2903
                     /locus_tag="ACHJZ2_004746"
     CDS             2376..2903
                     /locus_tag="ACHJZ2_004746"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO4570704.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGLGVRRAA"
     gene            2900..3082
                     /locus_tag="ACHJZ2_004747"
     CDS             2900..3082
                     /locus_tag="ACHJZ2_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4570705.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            3357..4121
                     /locus_tag="ACHJZ2_004748"
     CDS             3357..4121
                     /locus_tag="ACHJZ2_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO4570706.1"
                     /translation="MTTKISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLRVTLSNSQNLQPSLRGL
                     QISPKARSLILWTERGAARHAKMLETDRAWEVFEKLEDCYFSQCEKNTGKQEKKLNGL
                     SAKETDSLVWLWDYANRSQALFRELYPALKLIQSGYSGICHDYGYEFSYIIGRARGVL
                     INHTRDIDIYEPDGPTNLLAWERLKNKELPPSLHRY"
     gene            4196..4918
                     /locus_tag="ACHJZ2_004749"
     CDS             4196..4918
                     /locus_tag="ACHJZ2_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO4570707.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSQHGNVRISIERLAKRGVIQLP
                     SMQKVENKQTISPNKFTSVYIFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKRFAWMRDHKILIASGARRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            4918..>5430
                     /locus_tag="ACHJZ2_004750"
     CDS             4918..>5430
                     /locus_tag="ACHJZ2_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4570708.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNR"
BASE COUNT         1662 a         1136 c         1382 g         1250 t
ORIGIN      
        1 gatgagcttc tgtcaacatt atcatatgac tcagaaacag gaatatttaa atggctaaaa
       61 acaaattcag tagtaagagt aaaaggtagt attgctggag gtgttagtgg tggttatata
      121 tgcattagta taaataatgt tttgtataag gcgcatagac ttgcttggtt ctatgtatac
      181 aaaaaatggc ctcctaagtt tattgatcat gtaaatggga acagacttga taataggatt
      241 tcaaacctaa gactggcaac agaagagcag aatgcaagaa acattgtagg gaatagatta
      301 aacacatccg gtgcaattgg agtgtcttgg tataagccaa ctggcaggtg gaagtcttat
      361 gttggttata aaaataagac aatatcgtta gggtatttcg atagcaaaga agatgcagca
      421 ttcatagcag cactagcaag aaagaaacta tatggaactt atgcgagtaa agcacttaat
      481 tgcgagcatg agcttttatc tcaatttaat aatgatgagg ataaacttgc ggaatatctt
      541 aaggaaaaat ataaaaggac tcgaaagcgt gttaaaaaaa gataaaggcc tgctggtaat
      601 cgcaggcctt tttatttggg ggagagggaa gtcatgaatc tggacaagca agatgcacaa
      661 actattagct catacataag ggcatcaaga ccagattaca aaggtccggt attcgtagat
      721 ttatctcgcc ttgaatagat ttacatgtgg aaagcaaggt tacttacgca ttttgttatt
      781 cgaacgatga ctagcaacat tacaaaacca atgtaactgg aagaggtgaa tatgagcaca
      841 ctcgcagacc ttattcatgc cgatatggct gaagatggag caaggcgtaa taggtactgg
      901 aaatcatcga gccttccagt ttgtgaaaga ttcaatcaca ggccaaaacc aaaacgtagc
      961 cgacgagaca aagtgttgaa aaaactcatg caaattaaca tggctggttt tgtcagattc
     1021 gtgagtgaaa cgactaacgg tgattgatat ggacgaatca agaaagcagt ttgaggaata
     1081 cgttgccaaa aaattgagat taccattcga gatgataacc gaggcaagaa atggtgatag
     1141 gtacttcgca ttttcaagca tggatattcg tcactcctta aatgattggt gggctttatg
     1201 gcaggcatcg cgagcagcta ttgaactgga tatcgactgg cccgaatcga atgacgacct
     1261 ttggaaagat ggtgaagaag gtgcttatgc gatgggttat gaggatgggc gtgacaaaac
     1321 ggtaattgca gtaatgaaag ccatcagggc cgcaggaatc aaagaaaaga atttcgatta
     1381 agcaaatatc acttcaataa atcgctttta aggcatcaca atcgctctgt ggtgaggtaa
     1441 gcacgtgcaa ggtataccga taagcagcga gaatgaaaaa tgcgtcagaa tgcgtttgag
     1501 gaggttttaa gaaatgagta cgatagctga gcttgtcagg gctaattttc gtgaagagtt
     1561 ggtgcgttgg tatcggtatc gttcatcgtc cagtttgccg cttgatgagt tgtatgagca
     1621 ttcacctgcc gcacgacgct atccgcgtga ccgtgttctt cgacggttgt tcaaactcaa
     1681 caatgagttt cagcgcaaca gaattatccg gagtctggat tttaagtgaa ggagtgagca
     1741 tgagcgacct atcattaacc cagccaaagc taaaagaatg tccgttttgc ggcggtaatg
     1801 ctcgtctgtg ggttgaggcc ggaataaata ttgatgtgtg gggctatgca gaatgtgacc
     1861 tctgtgaagc cagggtggca tgggcaccat cagttgctgc ggctactgaa aaatggaacc
     1921 ggagagcagg agatgaagca aacctttctg cttcgcaacg aagcaatcag aaataacgcc
     1981 atagacgcca ttctctcact acccatcgac gacaagtcac cccacgaagt ccacgttaaa
     2041 gaacccaagc gcagcaaagc gcagaatgac cgcatgtggc cgatgctgaa cgatgtttcg
     2101 cgtcaggtgc tatggcatgg tcaacggctg gcgccggaag actggaaaga cctgttcact
     2161 gccctgtggc ttaagaccaa aaaactggag caaagaagtg cgcctggtat cgacggtggc
     2221 gttgtcatgc ttggcgtgcg taccagcaaa atgcgaaagg ccagcatgac tgagcttatc
     2281 gaaatcatgt tctggttcgg ctcagagcgc aacgtgcggt ggagtgatga ctcccggcga
     2341 gagtatgaat ggtcacaacg aaaagggaag gctgcatgac tatcaaatca aatacgccag
     2401 cacacgacaa ggactgctgg caaacgccgc tctggctttt tgatgcgctg gatattgagt
     2461 ttggattctg gctggattca gctgcgagcg acaaaaacgc tctgtgcgct cactggctaa
     2521 ctgaggctga cgacgcgcta aattctgagt ggataagcca cggtgcaatc tggaataacc
     2581 caccgtacag caatatcagg ccgtgggtgg aaaaagccgc tgagcagtgc atacaacagc
     2641 gacagacggt agtgatgctt gtgccagagg atatgtctgt cggatggttc agcaaggctc
     2701 tggagagtgt tgacgaagtt cgcatcatca ctgatggacg gattaatttt atcgaaccat
     2761 cgacagggct ggagaagaag ggaaacagca aaggctcaat gttgctgatt tggcgaccgt
     2821 tcatcagtcc tcgacggatg tttactaccg tatccaaagc ggcattgatg gcgatcgggc
     2881 tgggcgtcag gagggctgca tgaggcgaca gcgacgaagt atcaccgaca tcatctgcga
     2941 aaactgcaaa taccttccaa cgaaacgctc cagaaataaa cgcaagccaa tcccaaaaga
     3001 atctgacgta aaaaccttca attacacggc tcacctgtgg gatatccggt ggctaagaca
     3061 tcgtgcgagg aaaacaaggt gattgacgtg atgatttatt cggggctata ttcctcaaac
     3121 gccagcaaaa tctggcgtcg ggattggcgt cctggataga gaccgcgaca gatacacgcc
     3181 gcgagcgtgt tttttattgt cgtatgcacg cgcacatctg aattatggtg ggctgtgtgg
     3241 gggcggagag atccgcgccg gtcggtttcc cggttacgcc aaccctgcac agttcaccac
     3301 cagacgattg gcgtcgtcgg tggtgagtta ttaagaaacc accagagggc gtcattatga
     3361 caactaaaat ttctgttgaa actctctccc cgatcaccca taaccagatt cctgttatta
     3421 ccaccgaact tttggcgcag ctttacggca ctgagccggt gcgtattcgc cagaatcatc
     3481 atgagaacaa agtacgcttc gttgaaggga aacacttttt caaagttgtt ggtaatgacc
     3541 ttaaagaatt gcgggtagct ttaaactact cacaaaattt gcgggttact ttaagtaact
     3601 cacaaaattt gcgggttact ttaagtaact cacaaaattt gcaaccatct ttaagagggt
     3661 tacaaatttc cccgaaagcc cgctccctca tcctctggac agaacgcggc gcagcccgtc
     3721 acgcaaaaat gctcgaaacc gatcgggcgt gggaagtgtt cgaaaaactg gaagactgct
     3781 atttcagcca gtgcgagaaa aatactggca aacaagagaa gaagctcaac gggctttccg
     3841 caaaagaaac agacagcctt gtatggctgt gggattatgc caaccgctca caggcattgt
     3901 tccgtgagtt gtatcccgca ttaaaactga ttcagtctgg ctattccggc atatgccacg
     3961 actacggcta tgagttctcg tatatcatcg ggagggcgag gggcgtttta attaatcaca
     4021 cgcgggatat agatatttat gagcctgacg ggccgacgaa ccttctggca tgggaaaggc
     4081 ttaagaacaa agagttgccg ccttcactgc atcgctactg acaattgaca acttaacaaa
     4141 cccagcttcg gctgggtttt ttattgctga attttcaatg tgagaggaca tgacaatgaa
     4201 tgagctgata aatagcaatg ccatcaaaat gacaagcatt gaaatcgctg agttggtggg
     4261 aagccaacac ggtaatgtca gaatatcaat agaacgtctg gcaaagcgtg gggtgattca
     4321 acttccttca atgcaaaaag ttgaaaataa acaaacaatt agccctaaca aattcacaag
     4381 cgtgtatata ttcgaaggcg aacaaggtaa gcgagacagc attattgtcg tcgctcagtt
     4441 gtcgccggaa ttcaccgctc gccttgttga ccgctggcga gaactcgaag gggcaaccgc
     4501 gaaaatacca caaacctttt ctgaggcatt gcgccttgcg gccgaccttg aagaccagaa
     4561 ggctgaactg gagaaacagc ttgctctcgc agcacctaaa gttgagtttg ccgatcgagt
     4621 tggcgaggcc agcggaattt tgattggaaa ctttgcaaag gttgttggta ttggtccaaa
     4681 caaacggttt gcgtggatgc gcgatcacaa aatccttatt gcttcaggtg cccggcgcaa
     4741 tgtgccaatg caggaatata tggatcgcgg ctatttcaca gtgaaagaaa cagcggtcaa
     4801 tacaaatcac ggaatacaga tatcgttcac cacaaaaatc accgggcgtg gtcaacagtg
     4861 gctgacaaga aagctgctag ataacggaat gcttaaagta acaggggagg ctgcttaatg
     4921 gctaaaccag cgcgaaggaa atgcaaaatc tgtaaggaat ggtttcaccc ggcattctca
     4981 aatcagtggt ggtgcagccc ggaacacgga actaaattag cactcgaacg acgaaataaa
     5041 gaacgcgaaa aggcggaaaa aacagcagag aagaaacgac gacgagagga gcagaaacag
     5101 aaagataaaa ttaagattcg aaaactcgcc ttaaagcccc gcagttactg gattaaacaa
     5161 gcccaacaag ccgtaaacgc cttcatcaga gaaagagacc gcgacttacc atgtatctcg
     5221 tgcggaacgc tcacgtctgc tcagtgggat gccggacatt accggacaac tgctgcggcg
     5281 cctcaactcc gatttgatga acgcaatatt cacaagcaat gcgtggtgtg caaccaacat
     5341 aaaagcggaa atctcgttcc gtatcgcgtc gaactgatta accgcatcgg gcaggaagca
     5401 gtagacgaaa tcgaatcaaa ccataaccgc
//
LOCUS       DAZFBR010000202         5423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFBR010000202 DAZFBR010000000
VERSION     DAZFBR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.202"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..845)
                     /locus_tag="ACHJZ2_004751"
     CDS             complement(<1..845)
                     /locus_tag="ACHJZ2_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4570709.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSKPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICG"
     gene            1114..1230
                     /locus_tag="ACHJZ2_004752"
                     /pseudo
     CDS             1114..1230
                     /locus_tag="ACHJZ2_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1334..1410)
                     /locus_tag="ACHJZ2_004753"
     tRNA            complement(1334..1410)
                     /locus_tag="ACHJZ2_004753"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1374..1376),aa:Arg,seq:tct)
     gene            complement(1424..1500)
                     /locus_tag="ACHJZ2_004754"
     tRNA            complement(1424..1500)
                     /locus_tag="ACHJZ2_004754"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1464..1466),aa:Arg,seq:tcg)
     gene            complement(1508..1583)
                     /locus_tag="ACHJZ2_004755"
     tRNA            complement(1508..1583)
                     /locus_tag="ACHJZ2_004755"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1548..1550),aa:Ile,seq:cat)
     gene            complement(1612..2325)
                     /locus_tag="ACHJZ2_004756"
     CDS             complement(1612..2325)
                     /locus_tag="ACHJZ2_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4570710.1"
                     /translation="MVVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRALFLMH
                     YGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGD
                     TTDMFLMMACLSLFESDERILLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHM
                     SESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(2463..2659)
                     /locus_tag="ACHJZ2_004757"
                     /pseudo
     CDS             complement(2463..2659)
                     /locus_tag="ACHJZ2_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
     gene            complement(2946..3764)
                     /locus_tag="ACHJZ2_004758"
     CDS             complement(2946..3764)
                     /locus_tag="ACHJZ2_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO4570711.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALFFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(3916..4287)
                     /locus_tag="ACHJZ2_004759"
     CDS             complement(3916..4287)
                     /locus_tag="ACHJZ2_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4570712.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(4277..4648)
                     /locus_tag="ACHJZ2_004760"
     CDS             complement(4277..4648)
                     /locus_tag="ACHJZ2_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4570713.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(4661..>5423)
                     /locus_tag="ACHJZ2_004761"
     CDS             complement(4661..>5423)
                     /locus_tag="ACHJZ2_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4570714.1"
                     /translation="HGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQN
                     LAAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSV
                     YRESDIIPGEQTATSILKQRTKNIALPPHTHQQQNPPQEKTVVSIAVDPESPKSFMKR
                     PKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHH
                     NELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1554 a         1403 c         1145 g         1321 t
ORIGIN      
        1 tcaccacaaa tccacggcac atctgcagca ctgccaccat ggcactgcgc gttaaacacg
       61 gagaggtcag cacgaaactg tgtcagcatg gctgtaaaca gcgcaggttg ctgtgcgtgg
      121 gtggcggcgc tcatgtcaaa ctctccctgc atccagcaca ccgccagcaa cacatttttc
      181 gggttcttct gtaatgcagc tttagtgcgc gcaatcaggt cctgatataa cggtttaccc
      241 acaccccagc gtgccgaatc ctggctggcc cccgtgtccg cactgaatgt cccctcagcg
      301 ccctgggtga atgcagaacc accacgacag catggtacca gcaggatccc cgcgttattc
      361 gggatatacg gaagcagttt tttggcaata tgtaagccct ggccgacaca gccgtactgc
      421 cctttgctca ggtctgcctt cggatgattc agcgtactca tatcctgcac atcatgcagg
      481 cagtggtcgg ccggaatgat gtcgttatac gtacaactct ctccacccgg agttaccgtg
      541 ctgcggcgcg ccagctgttt aatgcgcgga tccggagcat cgtaagaatc cggtaacgga
      601 agcccttcac cgtaggccat gccgttggac tgtccggcaa gcacaaccac gtagaaccag
      661 tccggcttag atgaagggcc gacctgtgga tctccttcaa tagccaccgc ctgcatcagt
      721 gtgtacggcg taatggcaac cggtccgccg tatggctgcc agccctcttt cagtttgtgt
      781 gtcagctttt ccgcaaggtc tgacggcgac gccgccctga caacatcata gtgtttaaat
      841 gccatgaatc ctcccggccg ggataatatt gtgagtaaaa tgaggagcgg gctgaagtcc
      901 ggaagttaca ggacaatggc agaagagaga caacagcccg caatacgaaa aaggccgcgc
      961 tattgcgcag agtgattact gtcggatatt attcgccagc tgaaatatta cttcacgttt
     1021 tgttgtttat tccttgccgc ccgcgtctcc ctgcgcgggc tttttttgtc cataagaaag
     1081 cccctccgga gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc
     1141 ccggtgaatt cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt
     1201 gcccctccgc acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca
     1261 gacaacagtc aaccgcctga attgtgagac atttaaaaaa aggcccgcaa aagcgagcca
     1321 gggaaaataa gtgtggcgcg ttgtactgga ttcgaaccag tgaccgattg cttagaaggc
     1381 aattgctctg tccggctgag ctaacaacgc aggatacaga taatggaccg ccttcgggga
     1441 cccgaactcc gcgcaaccag cttcgaaagc tggcgctctt tcctgatgag ctaatggcgg
     1501 tatgtgatgg tggcccttgc tggatttgaa ccagcgacct ggtgattatg agtcgctcgc
     1561 tctcaccact gagctaaagg gctgggtcaa aaaataataa tcagatgaaa atcaataatc
     1621 aagcccttgc ctggatacat atctgtctgg cgggaagcca taatagcggt gaaatacaga
     1681 aataaagtag gaccagcttg aataaccgca tttttctgct acagcctgtc catatccatg
     1741 ccgggaacat aacatattga cagcaacacg catccgctct tccagcaaca agcgactgaa
     1801 catgtgccct tcatttttca gttttatctt taacaaactc tcactcatat gcaggcgtaa
     1861 cgcaatcgca ccaagcgtcc agcttgctga tatatctgtc tgaattatcg ccctgacttt
     1921 ggcacttata ctggataaac atccacttaa aaataataat atccgttcat ctgattcaaa
     1981 cagcgacagg catgccatca taagaaacat atccgtggta tctccggaaa atctctggct
     2041 ggtaattaaa gcctcagcca acgcagggtt gttgggttcc agtgacagat aaagcggaac
     2101 gtcagtcaga tgagttcttg tcagcttatg ctgaatttcc agatattgac ttactatgga
     2161 atggtttata tcgaaaattt taactttgcc ataatgcata aggaaaagcg ccctgatgca
     2221 tttggtggcc agaacgactg agccgggctt aagtgacaac gtatcctttt caagaaaaat
     2281 attaattggg gagcaaacca tgataactga acagacaaca accattataa ttttactttc
     2341 attagcaatt ggttagttca attatagccc caaaaggtaa attatcatca acacataagc
     2401 aaaggactga caggtgccgc taacacccac cagccgccca tttaccacaa ataaaaaagc
     2461 cttcaggact gaaggcgtct gtaacaaccg aactgatagt ctgccagacc cgcataacca
     2521 gctgggtcag tattaactgg cagcgctcgc gtgaaaggta cgtattctgc gcaatctccc
     2581 cgaccgtcgc cggttcggtg acgcttaatt catcaaacac aactctggcg gtttctgtca
     2641 tatcctgctg tttcagcatg tctttttacc ctttccggtt aacgtgacac accaataact
     2701 cttgtcgaaa aagccagcaa gctgaaagac ctgtattaat aactaccagc acatttaacg
     2761 cactgcgcta ctttgcgggc acaaaaaacc cgctcagagg cggggtcaag ctatgcggcg
     2821 aaataaccac tcttaacagc atacctgatt ttttacgtac gtaaatgctt tgccgtgcat
     2881 atttttcatg caaatgtcac gtcctgctat ttttcagtct tataaattta aaaccataga
     2941 aaaaatcaat tatgttttaa aaatggatag gtaaagaata acaagtgaca cagattcaaa
     3001 ccaaaatgga aaagggtggc aacccacaaa cgcccactcc acatccatga caatccatac
     3061 acaacaccag ataacgtggc aaataataca agtaaagcac cacctgaata gtgataaaaa
     3121 ccaaacaaca aagccgccac aattaatgca accaatggag acgttacttc tgatagccgt
     3181 gattgaatat accctctaaa taatgattcc tctgccagag acacaaaaaa caaattagcc
     3241 aatataaact ctggcaacca ctcaggaaaa tgaatctctg gctttaatcc accaaaaaaa
     3301 acagccaaaa acaggataag aggaacagag agcgacagag ccccccactt ccacaaagac
     3361 acttctgatt ttacttcttt tttaaacaaa gaagatgtac acaggactaa caaaaatggc
     3421 accagcgctt tatcaaaatt aaaatacatt gtatagggag tactttgagg gccaacagta
     3481 acagaattta gcactacagg attgtgaaac cctggccata gatggaaaaa taatgctatg
     3541 gctgataaaa ctatgccaac ttcatatata gatttagccc aggcgttata tttccagttg
     3601 aacttcaaaa taataaaaaa aacgattgta gcaacaaaaa ataacaccga ccaatcaata
     3661 atatcattaa gcacagccag gacaacagac accgtcaaca atgaaaaagc cactacctta
     3721 tgccaactga gagttgacag tgacagtact aacacaatcc acataagcac tccttttatt
     3781 taatgatgaa gattgattat caatattttc aattcaccag gcaacatttt atctaccttc
     3841 cacaatacat gaccatcaaa aaataaacat gttaattata aacacagaaa acataaccct
     3901 catcactata tatccctacc gcatatccat atctaaccgg acattcagag ccataagcat
     3961 tccttcaata ataccttccg ctttataaag ccttttacca ataagcccat cagaacatct
     4021 atgcttacgt gcaagagcca taaatgtcat tccacctacg taataatcca ccaacaaatc
     4081 gtgcaaatac tgattattct tgtttaatcg tgccatacaa ccacaaatta tcatggcgtc
     4141 atcatcagaa cacttaggac gtgatttcac tttagtcggg attaatcctt taaaaccagc
     4201 agctatcgag ggccatgtta catcttcatg attatttgct gcccatgccc cccatcgctc
     4261 aagaaccatc tggatatcac gcgccatcgt taccacctct gatttcgtaa atcttcacgc
     4321 ccagccgtcc acctggcaca ggctgaccgc gcacaatatt gatttcatca aactgctcgt
     4381 catcaatgag cacttccgca tgcgtcagcg catccagcgg tgctttcaga atgttgtcca
     4441 ggtcacggcg gcgcttatcc ggtggttctg caataatttt tattgccagc cgtccggaca
     4501 ggcttaattt cagccgctgc tggcgaacaa taagcgccac agcccggcga taacgctcac
     4561 cggcttttga tacaaaatat gtgctgccac gacgtcgcca gtaggtgttc accgtcggcg
     4621 ggtaaggcaa aacaaactct atacgcatca gtaacctctt ttacccgagc acgccggttg
     4681 caaaagcgtg atcaagaaaa cgaaaaatta aatcaacctg ggaaccatgc ttttcttcga
     4741 acgccagcgg atccgcatga agctcgttgt gatgctcccg acacagcggt agcgtgaaaa
     4801 tatcgtggga ttttgttccc attccaccct gaccatgacc aatcagatga tgaggatcgt
     4861 ccgctggctt accacaacac gcacacggct gtgtctttac ccagcgtgtg tatttctcat
     4921 ttacccagcg gcgacgttta ggtcgtttca tgaaggattt cggagactcc ggatcaacgg
     4981 caatgctgac caccgtcttt tcctgtggtg ggttctgttg ctggtgggtg tgaggcggta
     5041 gcgcaatatt ttttgtgcgc tgcttcagta tgctggtggc ggtctgttct cccggtatga
     5101 tgtcgctttc acggtatacg gagcggattt tttccgccgg taatcccaga gaacgacgcg
     5161 atactgcctc aggtaatgcg tccaccacct gattgcagac tgcccaccag gataattcag
     5221 ccagcgataa ttcccgttcc tgtatgccat tcattgcgtg gcggatgacg tcaatcatcc
     5281 aggcggccag attctgttga gcaagttgct caagtgattc ggatgtctgg tcgcgcagct
     5341 ggttgtcgca gtgccagcac aacaccatcg cgccggtacc gtaacgatgt atgacagttt
     5401 cgctgtgatg ataatcacca tga
//
LOCUS       DAZFBR010000203         5355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFBR010000203 DAZFBR010000000
VERSION     DAZFBR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.203"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            184..4086
                     /gene="espP"
                     /locus_tag="ACHJZ2_004762"
     CDS             184..4086
                     /gene="espP"
                     /locus_tag="ACHJZ2_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFO4570715.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAIITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGSSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4342..4823)
                     /locus_tag="ACHJZ2_004763"
                     /pseudo
     CDS             complement(4342..4823)
                     /locus_tag="ACHJZ2_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1616 a         1161 c         1203 g         1375 t
ORIGIN      
        1 tgagttcaca gataaaacac tctccaggaa aaccggggcg gttcagtgtt tgttatccgt
       61 atgataacaa acacataaag gagtaaacat ttatctggac gatgattaca atgttcacga
      121 ctgtcagaga cacacctctg atacattaga aaaaccaatg tttcccttaa aaatggagct
      181 tatatgaata aaatatactc tcttaaatac agccatatta caggagggtt aatcgctgtt
      241 tctgaattat ccggcagagt atcatcaaga gcaactggta agaaaaaaca caaacgcata
      301 cttgcattat gttttttagg cttattacaa tcctcatatt cttttgcgtc acagatggat
      361 atttcaaatt tctacatccg tgactatatg gattttgcac agaacaaggg catatttcag
      421 gctggcgcaa caaatattga aatagtgaag aaagatggct ccaccctgaa actaccggaa
      481 gtaccatttc ctgacttctc accggttgca aacaaagggt caaccacatc tattggtggt
      541 gcatacagta tcacagccac acacaatacg aaaaaccacc actcagttgc gacgcaaaac
      601 tgggggaaca gcacgtacaa acaaactgac tggaatactt cacatcctga ttttgcagta
      661 tcccgacttg acaagtttgt tgttgagacc cgaggtgcga ctgaaggcgc agatatttcg
      721 ttatcaaaac agcaggcact tgaacgttac ggggttaatt ataaaggaga aaagaaactt
      781 atcgcattca gagccggctc tggtgtggta tccgttaaaa aaaatggacg cataactcca
      841 tttaatgagg tttcttataa gccagaaatg ttaaatggct ctttcgttca cattgatgac
      901 tggagtggat ggctgatatt aaccaacaac cagtttgatg agtttaataa cattgcctct
      961 cagggtgaca gcggttcagc actgttcgtc tatgataacc aaaagaaaaa gtgggttgtc
     1021 gctggaactg tctgggggat ttataattac gccaatggca aaaaccacgc agcatacagt
     1081 aaatggaacc agacaaccat tgacaacctg aagaacaagt attcttacaa cgtggatatg
     1141 tcaggggctc aggttgcaac cattgaaaat ggaaaactga caggcactgg ctcagacacc
     1201 accgatataa aaaataagga cttaatattt actggcggtg gagatatcct cctgaaatcc
     1261 tcttttgata atggtgctgg cggtcttgtc tttaatgata aaaagaccta tcgagtaaac
     1321 ggggatgatt tcacctttaa aggtgccggt gttgatacaa gaaacggcag caccgttgag
     1381 tggaatatcc ggtatgataa taaagacaac cttcacaaaa ttggtgatgg cacattagat
     1441 gtccgaaaaa cccagaacac caacctgaaa acaggtgagg gtcttgtcat tcttggagct
     1501 gaaaaaacat tcaataatat ctacataacc agtggtgatg gaactgtccg actgaatgca
     1561 gaaaatgcac tgtctggcgg tgaatacaac ggtattttct ttgcgaaaaa tggcggaact
     1621 cttgacctga acggatataa tcagtctttc aataaaattg ctgcaactga ttcaggtgct
     1681 ataataacca atacgtcaac caaaaaatcc attttatccc tgaataatac tgctgactat
     1741 atctatcacg gcaacataaa cgggaatctg gacgtacttc agcatcatga gacgaaaaaa
     1801 gagaaccgtc gtcttattct tgatgggggc gtggacacaa caaatgatat aagcctgcgt
     1861 aatacacaac tgtccatgca gggacatgcc actgaacatg ccatttatcg ggatggagct
     1921 ttctcttgtt cactaccagc tcctatgcgc tttttgtgtg gcagtgatta tgttgcagga
     1981 atgcaaaata cagaagctga tgctgtaaaa caaaacggaa atgcctataa aaccaacaat
     2041 gctgtctctg atttatcgca gccagactgg gaaaccggaa cattcagatt tggaacgcta
     2101 catcttgaaa attccgattt ttctgttggt cgtaatgcaa atgtaatcgg ggacattcag
     2161 gccagtaaat caaacattac tattggtgac actacagcat atattgattt gcatgctggt
     2221 aaaaatatta ccggtgatgg ttttggcttc cgccagaata ttgtgcgtgg aagctcacaa
     2281 ggagaaacgc tgtttacagg agggatcaca gcagaagaca gcactatcgt tattaaagat
     2341 aaagcaaaag cattattttc aaattatgta tacctgctga acacaaaagc aaccatagag
     2401 aacggtgctg atgtgacaac tcaaagtggt atgttctcca cgagcgatat cagcatctct
     2461 ggtaatctgt ccatgacagg caatcccgac aaagacaata aattcgagcc ctcaatatat
     2521 ctgaatgatg cttcttatct actgactgac gactccgcca gactcgttgc caaaaataaa
     2581 gcatctgtgg tgggagatat acactccact aaaagtgcat ccatcatgtt tggtcatgat
     2641 gaaagcgacc tctcgcagtt gtctgacaga acctcaaaag ggcttgcact tggtctttta
     2701 ggtggctttg atgtctcata tcgcggttca gtcaatgccc cgtcagcatc tgccactatg
     2761 aacaacacct ggtggcaact aaccggagat tctgcgctga aaacactgaa aagtacaaac
     2821 agcatggtct atttcactga cagcgcaaac aataagaaat tccatacgct gacggtcgat
     2881 gagctggcaa ccagcaacag cgcctatgcg atgcgtacaa acctttctga atcagacaaa
     2941 ctggaggtca aaaaacactt gtctggtgag aacaatattt tactcgttga tttccttcag
     3001 aaaccaacgc ctgaaaaaca actgaatatt gaactggtaa gcgcgccaaa agacaccaat
     3061 gaaaatgtct ttaaagccag taaacaaacc attggtttca gtgatgtaac gccggtcatt
     3121 acaaccaggg aaaccgatga caaaataaca tggtcactga caggctataa cacggtagca
     3181 aacaaggaag caacccggaa tgccgccgcc ctgttctctg ttgactataa agcgtttctg
     3241 aacgaggtca acaacctgaa caaacgtatg ggtgacctgc gtgatatcaa cggcgaagcc
     3301 ggtgcatggg cacgcatcat gagcggtacc ggctctgcca gtggtggttt cagtgacaac
     3361 tacacgcacg ttcaggtcgg ggtcgacaaa aaacacgagc tggacggact ggatttgttt
     3421 accggtttca ctgtcacaca cactgacagc agtgcctccg ccgatgtttt cagtggtaaa
     3481 acgaagtctg tgggggctgg cctgtatgct tccgccatgt ttgattccgg tgcctatatc
     3541 gacctgattg gcaagtatgt tcaccatgat aatgagtaca ctgcaacctt tgccggactc
     3601 ggaacccgtg attacagcac gcattcatgg tatgccggtg cagaagcggg ctaccgctat
     3661 catgtcactg aggatgcctg gattgagcca caggctgagc tggtttacgg ttctgtatcc
     3721 ggtaaacagt ttgcatggaa ggaccaggga atgcatctgt ccatgaagga caaggactac
     3781 aatccgctga ttggccgaac gggtgtggat gtgggtaaat ccttctctgg taaggactgg
     3841 aaagtgacag cccgtgccgg tctgggctac cagttcgacc tgctggctaa cggcgaaacc
     3901 gtattgcggg atgcatctgg tgaaaaacgc atcaaaggtg aaaaggacag ccgtatgctg
     3961 atgtccgttg gcctgaatgc agaaatcagg gataacgtcc gctttggact ggagtttgag
     4021 aaatccgcct ttggtaagta caacgttgat aatgctgtca acgctaattt ccgttactcg
     4081 ttctgatact ccgtattcaa aagggcctgc gggcccttta cgctcagtgc ctgccgccct
     4141 gatatgtctg agagacattt ccgggatgtc agtcgactgg tttacttaac tgcgatgact
     4201 aaagctggca gtaatgccgg cttttttacg gccacatacg tcttcctctc atccggaact
     4261 gtgatacaaa tcctgcatac aggattgacc gccggttcct gcaaatggca cattttcagt
     4321 aagatagcga aggttacatt tgtcactggt taacataccg atggtaaaag gggcaagcag
     4381 cgccagcaat tcatcagccc gggggccaaa agtgtaggcc agaacgccac cagttttcgt
     4441 gttgtacgcg taccagagcc tgtgctgtca cgctttactg ccgataaaac tccattgctc
     4501 atcaagttcg cagatgagtg ccatatcagc atgagcgacc ggggatgacg ttattcttcg
     4561 tggcgagcgt tttttaaagt ccgaatgaca gtattaatgc cgattttcag cgtacgggca
     4621 gtatcgcgaa ctccggcacc gttgaaaggc atttcagtga tttggtcttg aactcccggt
     4681 ttacgggcct cgtaacggta agtgagctga aacactcgat gacagtcacg gcagcgaaat
     4741 cgatcatgtc cttttgggtt cttcccgtga cggtaaacct gaacggactg gcaacggggg
     4801 caatgaaggt taactgtgac cattaaagaa ccttaaaagt gactattata cccctaattc
     4861 aactaattgg aggcattacc atgcattagt gcaagcgcct tgtggattct tttgctaatg
     4921 gcggaaacat attgtacaat attgcattta tccctcaata aataaatgag ttatcagatt
     4981 gcaaaaatct gttatcaaac taattcaaat aaaaagagat tgatacgacc aacgtacaca
     5041 aaaattttcg atattcgaac taaataacag ctcctaatac cgctacactt tagctatcca
     5101 gtctgcgcct cagaaatgga cgatgccgtt gaaggactgg tgtatggcaa tgagccgctt
     5161 tattatcgaa ttcggtgacc gcttggacgg tcacttcgga gaaaaggcat ttacacagca
     5221 tcctaaacag gttcagcata ggtgatatac cgccttttca ttttcttctc cattatttat
     5281 aagggaggtg tattattatg caatattatt ggttttatcc attgttttta gttcatgaat
     5341 gtttgcaaaa aaaaa
//
LOCUS       DAZFBR010000204         5303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFBR010000204 DAZFBR010000000
VERSION     DAZFBR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.204"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..80)
                     /locus_tag="ACHJZ2_004764"
     CDS             complement(<1..80)
                     /locus_tag="ACHJZ2_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570716.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYAR"
     gene            complement(77..643)
                     /gene="yecD"
                     /locus_tag="ACHJZ2_004765"
     CDS             complement(77..643)
                     /gene="yecD"
                     /locus_tag="ACHJZ2_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO4570717.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            953..2725
                     /gene="aspS"
                     /locus_tag="ACHJZ2_004766"
     CDS             953..2725
                     /gene="aspS"
                     /locus_tag="ACHJZ2_004766"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO4570718.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            2843..3295
                     /gene="nudB"
                     /locus_tag="ACHJZ2_004767"
     CDS             2843..3295
                     /gene="nudB"
                     /locus_tag="ACHJZ2_004767"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO4570719.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            3324..4064
                     /gene="yebC"
                     /locus_tag="ACHJZ2_004768"
     CDS             3324..4064
                     /gene="yebC"
                     /locus_tag="ACHJZ2_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4570720.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            4099..4620
                     /gene="ruvC"
                     /locus_tag="ACHJZ2_004769"
     CDS             4099..4620
                     /gene="ruvC"
                     /locus_tag="ACHJZ2_004769"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO4570721.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4622..5224)
                     /gene="yebB"
                     /locus_tag="ACHJZ2_004770"
     CDS             complement(4622..5224)
                     /gene="yebB"
                     /locus_tag="ACHJZ2_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO4570722.1"
                     /translation="MNINYPAEYEIGDKVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1333 a         1306 c         1432 g         1232 t
ORIGIN      
        1 cgggcatact cttcaaggct ggtgatcccc aaccgcaccc atttaggatg cgaccattgc
       61 ggtagaccga tgtaaatcat aacgcgttga ggatctcttc cacgctacgc acacgggcga
      121 tgcgcgggta gatatggttg atactgttat tgtgctgctc ggcgctagcg gcactacagg
      181 catcttcggc aatcaccaga ttaaagccaa gttcccaggc attgcgggcg gtggattcaa
      241 ctccgatatt ggtcgagatc ccacataaca ctattgtatc gatacctcgg cggcgtaatt
      301 gcaactccag atccgtaccg taaaacgcac cccactgacg tttgattatt tcgatatcac
      361 tgtcagttgc acctaatgca gctggatgtt gccaccagtt ttcgggcaac actttagcgg
      421 gggaaggggc atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaacgc
      481 gcaccagaaa cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat
      541 taactacctc atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat
      601 cgatcaccac cagcgcagtg gttttagcat taagttcaag catgatgact cctgaaaaaa
      661 caaaatcgtg cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa
      721 tttgtatgag ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc
      781 gatataaagt ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt
      841 tccagtataa tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg
      901 gtcacctgcg cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac
      961 agaatattgt ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg
     1021 ggtcaaccgt cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg
     1081 tatcgtgcag gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact
     1141 gcgtaatgag ttctgcattc aggtcacggg caccgtacgt gcgcgtgacg aaaaaaatat
     1201 taaccgcgat atggcgaccg gcgaaatcga agtgctggcg tcctcgctga ctatcatcaa
     1261 ccgcgcagat gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa
     1321 ataccgctac ctcgacctgc gtcgtccgga aatggctcag cgcctgaaaa cccgcgctaa
     1381 aatcaccagc ctggtgcgcc gttttatgga tgaccacggt ttcctcgaca tcgaaactcc
     1441 gatgctgacc aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca
     1501 caaaggtaaa ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat
     1561 gtccggcttt gaccgctact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc
     1621 tgatcgtcag cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca
     1681 agtgcgtgaa gtgatggaag cgctggtgcg ccatctgtgg ctggaagtga agggcgtgga
     1741 tctgggcgat ttcccagtaa tgacctttgc ggaagccgaa cgccgttatg gttctgataa
     1801 accggatctg cgtaacccga tggaactgac cgacgttgct gatctgctga gatctgttga
     1861 gtttgcagta tttgcaggtc cggcgaacga tccgaaaggt cgcgttgccg ctctgcgtgt
     1921 tccgggtggc gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat
     1981 ctacggcgcg aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg
     2041 tatcaacagc ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg
     2101 tactgccgcg caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc
     2161 cgacgcgatg ggcgcactgc gcctgaaagt gggtaaagac cttggtctga ccgacgaaag
     2221 caaatgggca ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg
     2281 cctgacggca atgcaccatc cgttcacctc accgaaagac atgacggctg cagagctgaa
     2341 agctgcaccg gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt
     2401 gggcggtggt tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct
     2461 gggtatcaac gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ctctgaaata
     2521 cggtactccg ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac
     2581 cggcaccgac aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct
     2641 gatgactgaa gcaccgagct ttgctaaccc ggctgcactg gctgagctga gcattcaggt
     2701 tgtgaagaag gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat
     2761 cgaggcggca actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga
     2821 aggcagtcaa cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt
     2881 agtggtcatc tacgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgatgaccc
     2941 cgatttctgg cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc
     3001 catgcgcgaa gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat
     3061 tgactgtcag cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc
     3121 gggcgtgacg cgtaatacgg aatcatggtt ctgtcttgcg cttccgcatg agcggcagat
     3181 cgttttcact gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac
     3241 taagtcctgg agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg
     3301 cagggctatc tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc
     3361 gtaaagctgc gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg
     3421 taaccgcggc taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcagcaa
     3481 ttgataaagc actgtctaac aacatgaccc gtgacactct gaaccgcgca attgcacgtg
     3541 gtgtgggcgg tgatgatgat gcgaacatgg aaaccatcat ctacgaaggt tacggtcctg
     3601 gcggcacggc aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag
     3661 tgcgtcatgc attcagcaaa tgtggcggta acctcggtac tgatggttcc gtagcctatc
     3721 tgttcagcaa aaaaggcgtc atctccttcg agaaaggcga tgaagacacc atcatggaag
     3781 cagcactgga agcaggtgct gaagacgttg tgacctatga tgacggcgcg attgatgtct
     3841 acactgcatg ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag
     3901 cagacagcgc ggaagtttcc atgatcccgt ctaccaaagc tgatatggat gcagaaaccg
     3961 caccgaaact gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt
     4021 accataacgg tgaaatctct gatgaggtcg cagcgactct ctgatgaggc ctgctaaaca
     4081 gcaaaacgga gacgcgtgat ggctattatt ctcggcattg atccgggttc gcgcgtgacc
     4141 ggctacggcg tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata
     4201 cgcaccaaag tggatgattt accgtctcgt ctgaaactca tctatgcggg cgtgacggaa
     4261 atcatcaccc agttccagcc cgattatttc gccattgaac aagtctttat ggcaaagaac
     4321 gctgactcag ccctgaaact gggccaggcg cgcggcgtgg cgattgtggc ggcggtgaat
     4381 caggagttgc cagtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatt
     4441 ggtagtgccg aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct
     4501 aatccacagg cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt
     4561 cagaatgcga tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa
     4621 gttataccgc ctcggtcagt tccggctgag gcgtttccac tccctccgca tgaatcaaca
     4681 ccaaacctgg atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag
     4741 aacccaggaa ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacaatt
     4801 ctccaaacgt ctctatttca cccaccggaa tacatagcgc ttctttataa atatcaaaaa
     4861 caaattttga acagaactgg cgcgaagatt cgtatttaaa accggtgtgg taaagtttgc
     4921 gtagccggga gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt
     4981 ctaatcgctt tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg
     5041 tgatggttga aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga
     5101 taatgatccc gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg
     5161 cagcacctat acatgtaaag actttatctc caatttcata ttcagcaggg taattaatat
     5221 tcacgttgtc attccattga aatagatacg aatatacgta atagcataaa aaatttattt
     5281 tttcaggagg cgcaatgtat att
//
LOCUS       DAZFBR010000205         5166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFBR010000205 DAZFBR010000000
VERSION     DAZFBR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.205"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            111..3026
                     /locus_tag="ACHJZ2_004771"
     CDS             111..3026
                     /locus_tag="ACHJZ2_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4570723.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAVSQQSA
                     ATSASTATTKASEAASSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSSDITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHS
                     VSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            3026..3607
                     /locus_tag="ACHJZ2_004772"
     CDS             3026..3607
                     /locus_tag="ACHJZ2_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4570724.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(3727..4617)
                     /locus_tag="ACHJZ2_004773"
     CDS             complement(3727..4617)
                     /locus_tag="ACHJZ2_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFO4570725.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(4636..5142)
                     /locus_tag="ACHJZ2_004774"
     CDS             complement(4636..5142)
                     /locus_tag="ACHJZ2_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4570726.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
BASE COUNT         1393 a         1249 c         1312 g         1212 t
ORIGIN      
        1 ctggcgcact gacgggttca tcgtgggtgt tggttataaa ttctgattag ctaggtaaca
       61 cagtgttatg acagcccgcc ggttcaggcg ggcttttttg tggggtgaat atggcagtaa
      121 agatttcagg tgtactgaaa gacggcacag gaaaaccggt acagaactgc acaatccagc
      181 tgaaagcaaa acgtaacagt accacggtgg tggtgaacac gctggcctca gaaaatccgg
      241 atgaagccgg gcgttacagc atggacgttg agtacggtca gtacagcgtt attctgttgg
      301 tggaagggtt cccgccgtca catgccggga ccatcaccgt gtatgaagat tctcaacccg
      361 gtacgctgaa tgattttctt ggtgccatga ctgaggatga tgtccgtccg gaggcactgc
      421 gccgttttga gctgatggtg gaagaggtgg cgcgtaacgc gtccgcggtg gcacagaaca
      481 cggcagccgc gaagaagtca gccagcgatg ccagcacatc agcccgtgag gcggcaaccc
      541 atgcgactga tgctgcggac tcagcacgcg cagccagcac gtcagccgga caggccgcgt
      601 cgtcggctca gtcagcgtct tccagcgcag gaacggcatc aacaaaggct actgaagcat
      661 caaaaagtgc tgccgctgca gagtcctcaa aaagcgcggc ggctaccagt gccggtgcgg
      721 cgaaaacgtc agaaacgaat gcggcagtgt cacaacaatc agccgccact tctgcatcca
      781 ccgcgaccac gaaagcgtca gaagctgcct cctcagccag ggatgcgtcg gcttcaaaag
      841 aggcggcaaa atcatcagaa acgagcgcag cctcgagcgc cagtagtgca gcctcctcgg
      901 caacggcggc aggcaattcc gcgaaggcgg ccaaaacgtc tgagacaaac gctaagtcct
      961 ctgaaacggc agcagaacag agtgcctccg cagcagcagg ctcaaaaaca gcggctgcat
     1021 tatctgccag tgccgcgtca acaagtgccg ggcaggcctc agccagtgcc accgccgccg
     1081 gaaaatcggc agaaagtgcc gcatcgtctg cttcaacagc cacaacgaag gctggcgaag
     1141 ccactgaaca ggccagcgca gcagcgagtt ctgcttccgc agcgaagaca tccgaaacga
     1201 acgcgaaagc gtcggaaacc agcgcagaat cctcaaaaac ggctgccgca tcgtcagcca
     1261 gttcggcggc gtcatcggca tcatctgcgt ctgcttcaaa agatgaggcg accagacaag
     1321 cgtcagcagc gaagagcagc gccacgacgg catccacgaa ggcgacagag gcagctggta
     1381 gtgcgacggc agcagctcag agcaaaagta cggcggaatc tgcagcaacg cgcgctgaga
     1441 cagcggcaaa acgggcagag gatattgcat ccgccgtggc gcttgaggat gcgagcacga
     1501 cgaaaaaggg gatagtacag ctcagcagtg cgactaacag cacttccgag tcactggcgg
     1561 caacgccaaa agccgttaag gccgcgtatg agctggctaa cgggaaatac accgcacagg
     1621 atgcaacgac agcacagaaa gggatagttc agcttagcaa cgcgaccaac agcacatctg
     1681 aaatgctggc ggcaacgcca aagtcggtaa aggcagccta tgaccttgct aacgggaaat
     1741 atactgctca ggacgctacg acagcacaaa aaggaattgt ccagctcagt agtgcaacca
     1801 acagcgcatc tgaaacgctt gccgcgacac cgaaagcagt gaaagcagct aatgataatg
     1861 cgaatggtcg ggtaccttct gcccgtaagg tgaatggtaa ggcgctttca tcggatataa
     1921 cactgacgcc gaaagatatt ggtacgctta actcaacaac aatgtcattc agcggtggtg
     1981 ctggttggtt caaattagca acggtaacca tgccacaggc gagttctgtt gtttcaatta
     2041 cgttgattgg tggcgcggga tttaacgtgg ggtcacctca acaggcaggt atatctgaac
     2101 ttgttttgcg tgcaggtaat ggtaatccga aggggattac tggtgcttta tggcagcgca
     2161 catcgacagg gtttacaaat tttgcctggg tcaatacatc tggtgatact tacgatattt
     2221 acgttgcaat cggaaattat gcgactggtg taaatattca atgggattat accagtaatg
     2281 ccagcgtgac gattcatacg tcaccagcat attctgctaa taagccggaa gggttaacgg
     2341 acggtacagt ttattcactc tatacgccat cagagcagtt ttatccgcct ggcgcaccaa
     2401 tcccgtggcc atcagatacc gttccgtctg gctatgccct gatgcagggg cagacttttg
     2461 acaaatctgc atacccgaaa cttgcagccg cttatccgtc aggcgtgatc cctgatatgc
     2521 gtggctggac gattaagggc aaacctgcca gtggtcgggc cgtattgtct caggaacagg
     2581 acggcattaa atcgcacacc cacagcgcca gcgcatccag tacggatttg gggacgaaaa
     2641 ccacatcgtc gtttgattac ggcactaaat ccacgaataa caccggggcg cacacgcaca
     2701 gtgtgagcgg tacagccgca agtgccggaa accatactca tagtgtcaca ggcgcatcag
     2761 cagtcagcca gtggtcacaa aatgggtcag tacataaggt agtgtctgcg gccagtgtga
     2821 atacaagtgc tgcaggagcg cacactcata gtgtcagcgg cacagctgca tctgcaggtg
     2881 ctcacgcaca tactgtcggt attggtgctc atacgcactc tgttgcgatt ggctcacatg
     2941 gacacaccat caccgttaac gctgcgggta acgcggaaaa cactgtcaaa aacatcgcat
     3001 ttaactacat tgtgaggctt gcataatggc attcagaatg agtgaacaac cacggaccat
     3061 aaaaatttat aatctgctga ccggaactaa tgaatttatt ggtgaaggtg acgcatatat
     3121 tccgcctcat acaggtctgc cagcaaacag tacctatatt gcaccgccag atattccggc
     3181 tggcttcgtg gctgttttca acagtgatga gtcatcgtgg catctcgttg aagatcatcg
     3241 gggtaaaacg gtttatgacg tggcttccgg cgacgcgtta tttatttctg aactcggctc
     3301 attaccggaa aatgttacct ggttatcgcc ggaaggggag tttcagaagt ggaacggcac
     3361 agcctgggtg aaagatgcag aagcagaaaa actgttccgg atccgggagg cggaagaaac
     3421 aaaaaacagc ctgatgcagg tagccagtga gcatattgcg ccacttcagg atgctgtaga
     3481 tctggaaatc gcaacggagg aagaaacctc attgctggaa gcctggaaaa agtatcgggt
     3541 gttgctgaac cgtgttgata catcaactgc acctgatatt gagtggcctg taatacctga
     3601 agtttaaaat aaaagcccgc ttaaaatatc gcgggcttcg aaatataaat gtattttcag
     3661 aggctagagc ttaggatatc tcagcggcaa ttaacttatg agggatagct aaataccaga
     3721 agataattag tttttactat ttatctttgt ttgtggttct ccttcagcaa gctcagcgcc
     3781 agtgacagga ttgatgtctt catgggattt caaccttgtt gccatagctt ttatcatagc
     3841 ttcttgcttg gtcgaaagat ttatcgaggc cttcccatcc ccaccgtcaa ccgctgaaac
     3901 tggatcggaa acatagtcaa agttttcatc actgttccaa ctacctctga cgtcttcacc
     3961 ttctgacatg ttgtaatata cgtttttgta tttctcaagt ggtggtaatt ttcctggtgg
     4021 aaaggtgtta cgaattgaat gtaatgcttt ttcaaatgaa agcatgtgtg ctgcttcccg
     4081 ggtcattaaa aatgccagag tgtcttttac tccaggatca tcagtaacat taatgagacg
     4141 ttcgtaaatg atctttgccc gagcttcagc tgcaatattt gaacgaagat cggccgtgac
     4201 ttcgccaata gtatcaacat aagctgcagt ccagggtact ccagctgaat tagttaatgc
     4261 tgggcctcct ccgtagagga gagaagttat atggctatca ttgccatttt gagttaagga
     4321 acggtaaagc tcagcttcat tctcagttcc ttcagcaagt gctccttttg cacctttgtt
     4381 gagcatacca acaagagaac caataatttc gagatgactt agttcttctg ttgctatgtc
     4441 catcagcata tcccttcggc ctgcatcttc atcacttaag ccttgagtga agtatcggca
     4501 tgctgctgca agctcaccct gtggcccacc gaattgttct aagagtagat tagccaagcc
     4561 tgggtttggc tcacttacac gtactgtata ttgaagtttt ttcacgtgtc taaacataat
     4621 gcctcctcaa aagaattatt ttttggcttc aactccatca atttcagatc tgaccatgaa
     4681 ttgctcagtg gtgtcaggaa tatggttaag aagccattca gccatttgtt tttcttcatt
     4741 aagaattgct tcaataatgg gtactgaagc tgtatcaccc gcatttttgg ctgctgcgag
     4801 aagagatgtg taacacgcaa tttcaaattg ttcgaacaca tagccactga tcgaaccttt
     4861 gacaatttca tcagatggaa aaattccacc aatggattgt ccaaacgcgg ccattttact
     4921 catagagtct ttaatgacag agcgcgaaat attattacga tcaagtatgc tttcaagctg
     4981 tgaaagctgg ttctttgttt cgctaatgtg ctgctcaatt ctggaacgta actcaggata
     5041 gttttctatc cggctggcca tagactcaag cattttttca gcttgttttt ccatcgcatg
     5101 tgcatcacga agccagtcat ggtaatgttc aattctattc ataatctgtt tatcctaaaa
     5161 gaaatt
//
LOCUS       DAZFBR010000206         4888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFBR010000206 DAZFBR010000000
VERSION     DAZFBR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.206"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(162..797)
                     /gene="yfdX"
                     /locus_tag="ACHJZ2_004775"
     CDS             complement(162..797)
                     /gene="yfdX"
                     /locus_tag="ACHJZ2_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO4570727.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1093..1368
                     /gene="ypdI"
                     /locus_tag="ACHJZ2_004776"
     CDS             1093..1368
                     /gene="ypdI"
                     /locus_tag="ACHJZ2_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO4570728.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSNSGGGSETSYKEPEKTSAM
                     TTTHSTKFQPSQAILFKMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(1445..1687)
                     /gene="yfdY"
                     /locus_tag="ACHJZ2_004777"
     CDS             complement(1445..1687)
                     /gene="yfdY"
                     /locus_tag="ACHJZ2_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO4570729.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            2040..2960
                     /gene="lpxP"
                     /locus_tag="ACHJZ2_004778"
     CDS             2040..2960
                     /gene="lpxP"
                     /locus_tag="ACHJZ2_004778"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO4570730.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3316..3387
                     /gene="ypdK"
                     /locus_tag="ACHJZ2_004779"
     CDS             3316..3387
                     /gene="ypdK"
                     /locus_tag="ACHJZ2_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO4570731.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3452..4690)
                     /gene="alaC"
                     /locus_tag="ACHJZ2_004780"
     CDS             complement(3452..4690)
                     /gene="alaC"
                     /locus_tag="ACHJZ2_004780"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO4570732.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         1266 a         1200 c         1100 g         1322 t
ORIGIN      
        1 acacgggatt attgcggttt actttgagga atataaaatg cgagtttata gaacagcgag
       61 cgccaaataa ttttatgacc acaacctgtc gtattgtggt cataaaaaat tgtcggctgg
      121 agggagttaa atcctttacc tcaatatact gcctgtcagc attagttcac aaacagtgct
      181 tcgctatcga cgatgattcc atcttccgca cctttaagcg ccaggtttgc ctcgtagtat
      241 tgctttttat caagcagttt ctgcgcatca gcaagtgcat tacgagtctg tttaagcggc
      301 atcagatatt ggttttccat tacaccaaca ccggccaggc gaagctcttc cattgcgccc
      361 tttttatccc ctttcgccat tttttcattg gcaattttta tcgcggcttc tttttcgggt
      421 gtcgctacat aactctcaga aattccgaca gatgcattaa tgacaatata ttggtcatca
      481 ttgacattgg tctttttacc tggcttagcg aactttgccc attcggtact gtcatctgac
      541 aacaaagctg aagcttcatt ggtcagttct tttgcctttt ccggatcgcc atgaaacaga
      601 gccaggcgcg ctacctgcac gtcacgcatc gcatataaac cttgttcaga gatacgctct
      661 gctgcagctg ccgttttctg ggtttgctga acttgctgag tttgctgttg tgccgctacc
      721 ggagcattat cagcagccca tacagttgaa gatgccagaa tagctgtgac catcgtagcc
      781 ataattaaac gtttcatact tacctcattc tttaacagtt ttagaaaacg ccgccatcat
      841 attgggtgag catgaaatga gaaatcagag agcaacggtc attttgaaag ggttatctta
      901 cagttgtagt tattatcgca agaagtattt tagtcttaca attgtaggaa tatgcttcct
      961 gtcacagaga actattctta tgtaccacaa agagatgtca tttattatat cgatgtaaat
     1021 attgattaat tatatgtaaa tacagccata acatcgtaca ttctcagtga accattttca
     1081 ggataacttt ctatgaaagt aaacttaata ctttttagct tatttttatt ggtctctatt
     1141 atggcatgca atgtttttgc attttctaat tctggtggtg gtagtgagac gagctataaa
     1201 gagccagaaa aaacgtcagc gatgacgaca actcactcta caaaatttca gccatcacag
     1261 gcgattttgt ttaagatgag agaagatgca ccaccattaa acctcacaga agaaaccacg
     1321 cccccttttc cgacaaaggc gaattatctt attcatcctg tgcgatagcg tctgtcacct
     1381 ggcaggtgaa caatcatgtc tgatgctgat gtgaacacct gcccaaatga accatcctga
     1441 cccactatct cctgccatac cccaccataa agcgaatcat caagccacaa gccggggcaa
     1501 gaaacataaa tatccccgtc accagttcat taccgccagt taaccacatg gtgaagtaat
     1561 aaatgagtgc agcgaggatc accatgccga agaaagaaga gaccgccgat accacgttca
     1621 gtacctgacc gatgtatccg cttacgcaga caatacacag ggcgagaaac atccataaat
     1681 aaaacatagc tcaacctcct gccgcgctaa aaaacctgta acttcagtca ctttaatagt
     1741 aagacaatcc ttaacctctg gcaatttttg ctaagcgtca atgaaaacag catgttaaat
     1801 gcaatactat tgcgtgcgga aaatttttta ctttgcacga ttaataatca gtagctgaaa
     1861 gcagtcagcc agtgataaag tacagatacc gcattcatgc tcctcgtggt tatgtcctga
     1921 ctagtcttta cactctttac aggaaccatt gtcgtacatg atggcccaac caattgagtg
     1981 taccgctgtc gacatggaat cttctgcgat acaacaattc gtatctacag aaggtaacta
     2041 tgtttccaca atgcaaattt tcccgcgagt ttctacatcc tcgctactgg ctcacatggt
     2101 ttgggcttgg tgtactctgg ctttgggtac agcttcctta tcctgttctc tgctttctcg
     2161 gcacgcgtat tggcgcaatg gcgcgaccat tcctgaaacg gcgtgaatct atcgcccgta
     2221 aaaacctgga actttgcttc ccgcagcatt ctgcggaaga acgcgagaag atgattgccg
     2281 aaaactttcg ttcactcggc atggctctgg tagaaaccgg catggcgtgg ttctggcccg
     2341 acagtcgcgt acgtaaatgg tttgatgttg aagggctgga taaccttaaa cgcgcacaaa
     2401 tgcaaaatcg cggcgtaatg gttgtcggcg tccattttat gtcgctggaa ctgggcggcc
     2461 gcgtgatggg actttgtcag ccaatgatgg ctacctatcg tccacataat aatcagctga
     2521 tggaatgggt gcagacccgt gggcgcatgc gctctaacaa agcgatgatc ggcagaaata
     2581 atctgcgcgg cattgtcggt gcactgaaga aaggtgaagc ggtatggttt gctcccgatc
     2641 aggattatgg tcgtaaaggc agctccttcg cgccgttctt tgcggtggaa aatgtcgcca
     2701 caaccaatgg cacctatgtt ctctcccgtc tctctggcgc agccatgttg accgtaacga
     2761 tggtaagaaa agcggattac agcggatatc gtttgttcat caccccagag atggaaggct
     2821 acccgacaga tgaaaatcaa gccgctgcct atatgaacaa gattatcgag aaagagatca
     2881 tgcgcgcacc ggagcagtac ctctggatcc accgtcgctt taaaacgcgc ccggtgggag
     2941 aatcgtcgtt gtacatttaa caaactgctc ttactctttc cctcctcaaa tcggccataa
     3001 taacctcatg gtttttatgg cttttttctt aagatttttt ccttcgctga atatctaatt
     3061 attaccgcca gaaattaaac tgtcgccgat atgagacacc tgtaagttac taaaagtaaa
     3121 taagaaaatg cctcttgtct cgtttcagtt ttaggcgtac attagcgccg tctcgagcag
     3181 tttagctcag aattctgggg tgatgcccac ggtaaaatac aggactttta aggtcgtttt
     3241 gcccggtgtc aactcactgt atcaggtgtg atgaagtcat tcaggcgtaa ccgtaattac
     3301 gcggagagat gtaaagtgaa atatttcttt atgggcattt cttttatggt catcgtttgg
     3361 gccggtactt ttgccctgat gatctaaaag caaactgtgc aatgaaaaac aggagccatc
     3421 aggctcctgt ttgcatttct gacgacggtg tttattccgc gttttcgtga atatgtttgc
     3481 tgctggcggg tagtaaaccg tcggcacgga acatcgcttt aatcccacga atcgcctgac
     3541 gaatacggtc gcggttttca atcagtgcaa agcgaacatg cgtgtcgccg tagtcaccaa
     3601 agccaatccc tggagagaca cagaccttcg cttcgttaag gagcttcttg gcaaattcca
     3661 gcgatcccat ggccgcatat ggttccggga ttttcgccca gacatacatc gaagccttcg
     3721 gcatttcgac catccagccc gcttcatgca gccctttaac caatacatcg cggcggcgtt
     3781 tgtactgttc ggcaatgtcg cgcacgcact gttgatcgcc ctccagcgcc gcaatcgccg
     3841 ccacctgcaa cggcgtaaaa gtgccgtaat cgtgatagct tttgattcgc gccagggcac
     3901 tgaccagcgt tttgttgccg accataaagc cgatacgcca gcccgccatg ttgtagcttt
     3961 tcgacagcgt aaagaactcg actgccacat cgcgtgcacc cggtacctgc atgattgacg
     4021 gcgctttcca gccatcgtag acgatatcgg cataggccag gtcatggacc accagcacat
     4081 cgtagcgttt cgccagcgcc actacttttt cgaagaactc cagttccacg cattgcgcgg
     4141 ttgggtttga cgggaagccg aggatcatca tcttcggttt cggataactt tcaagaatgg
     4201 cgcgctccag ttcgttgaag aaatcgacac cttccaccag cggcactgag cgtacctgcg
     4261 ccccggcaat caccgcgcca taaatatgga tcgggtagct tggattcggc accagcaccg
     4321 tgtcaccatg atccagcgtc gccagcatca ggtgtgccag cccttctttc gaaccaatgg
     4381 tgacgatggc ttctgattcc ggatcgattt caacgtcgta gcgatcctga taccagcggg
     4441 aaatggcgcg acgtaaccgc ggaatgccgc gtgaagtgga gtaaccatgc gtgtccggac
     4501 gctgggccac agtacataat ttttcgacga tatgcggcgg agtcgcaccg tccgggttac
     4561 ccatgctgaa atcgataata tcttcgccgc gccgacgcgc agccattttc agttcagcgg
     4621 tgatgttaaa aacgtagggc gggagacgat caatgcgcgt aaagcgacgt tcagggcgag
     4681 tgtcagccat aatttcctca gattaacgtt agcgcccgga ccgtccgagc gacgctgcca
     4741 cgatggtggc tgttttgaaa atagcctgat taatttctgt ctgtccagcg ttttagaaca
     4801 tttttattaa tgacgttaaa tgaggaactc tgctttaaaa acaggaaatt tatctgcgct
     4861 aacaagaaaa ttaccactca ttttatta
//
LOCUS       DAZFBR010000207         4883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFBR010000207 DAZFBR010000000
VERSION     DAZFBR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.207"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..124)
                     /locus_tag="ACHJZ2_004781"
     CDS             complement(<1..124)
                     /locus_tag="ACHJZ2_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570733.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLENGKCFRMVLKPWRE"
     gene            complement(121..222)
                     /locus_tag="ACHJZ2_004782"
     CDS             complement(121..222)
                     /locus_tag="ACHJZ2_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570734.1"
                     /translation="MRTYNPNSLLPSLMQKCTCGSLHPTFDLCGGEA"
     gene            complement(313..594)
                     /locus_tag="ACHJZ2_004783"
     CDS             complement(313..594)
                     /locus_tag="ACHJZ2_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570735.1"
                     /translation="MEDYLVFGLGYEGDIKNDEAGLDKISVVTKSVVRSTNSNEPVVY
                     QVTEFKEFNVVRHQAFDSEYYNIAFDVLPSRVRIDAAIRKYHPKKSSSA"
     gene            complement(638..886)
                     /locus_tag="ACHJZ2_004784"
     CDS             complement(638..886)
                     /locus_tag="ACHJZ2_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570736.1"
                     /translation="MHELFVLVLSTCASLSNMSGCSVEVVNVNTTKEPVNVFYSRKDC
                     EESMKNIMLNHALYHEVSGREPYMAKCEQVFVSKSLMK"
     gene            complement(1063..1347)
                     /locus_tag="ACHJZ2_004785"
     CDS             complement(1063..1347)
                     /locus_tag="ACHJZ2_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO4570737.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKVSTNMIFKECRQSAAMKRVLLVYRT"
     gene            complement(1344..2045)
                     /locus_tag="ACHJZ2_004786"
     CDS             complement(1344..2045)
                     /locus_tag="ACHJZ2_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4570738.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNEVNEIRRQWVLAFRENGITSMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGEAIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2042..3061)
                     /locus_tag="ACHJZ2_004787"
     CDS             complement(2042..3061)
                     /locus_tag="ACHJZ2_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4570739.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(3058..3597)
                     /locus_tag="ACHJZ2_004788"
     CDS             complement(3058..3597)
                     /locus_tag="ACHJZ2_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO4570740.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQIKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            complement(3628..3850)
                     /locus_tag="ACHJZ2_004789"
                     /pseudo
     CDS             complement(3628..3850)
                     /locus_tag="ACHJZ2_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
     gene            3961..4653
                     /locus_tag="ACHJZ2_004790"
     CDS             3961..4653
                     /locus_tag="ACHJZ2_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4570741.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVNHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
BASE COUNT         1212 a         1180 c         1106 g         1385 t
ORIGIN      
        1 tctcacgcca cggtttaagc accatgcgaa agcattttcc gttctccaga taaggctgga
       61 tctgctggcc gatagcagtg aagttaccgc gatgcaattt gataccgtct tgtgggaggt
      121 tcacgcttca cctccgcaga ggtcaaacgt tggatgcaaa gaaccacagg tgcatttctg
      181 catcagtgaa gggagaagag agtttggatt gtatgtgcgc ataaacgtcc ccgtttagcg
      241 cagaagtcac cggagttgtt caggctccga ggacattatt attgctggtt gataatggaa
      301 aatcaaagct tactaagctg aggaggattt cttggggtga tatttacgga ttgcagcatc
      361 aatacggacg cgggatggta agacatcgaa tgctatgttg tagtattcac tatcaaacgc
      421 ttgatgtctc acaacattaa attctttaaa ctcagtcact tggtaaacca ctggttcatt
      481 ggaattggta gaacgcacta ccgatttcgt taccacgctt atcttatcaa gccctgcttc
      541 atcgttcttt atatcgcctt cataacctaa accaaaaact aagtaatctt ccacgttaag
      601 ctcctataag agtttgttat atgtcgtttt tggccaatta tttcattaaa ctttttgaga
      661 caaatacttg ttcgcacttt gccatatatg gctctctgcc tgatacttca tggtatagag
      721 catgatttag cataatgttt ttcatgcttt cttcgcagtc tttcctgcta taaaagacgt
      781 ttaccggctc ttttgtggtg ttgacattca ctacttccac tgaacaacct gacatattgc
      841 tgaggctagc acaggtgctc aaaaccagca caaatagttc gtgcataaaa cgactccctt
      901 atctatgtgc tggttatatt gcccaaattc taatagaaat tacgactatt ttttaacggc
      961 agcaacatct tgtattccta aacttagcag aaggcggtgg gtaaagacgg taccatagtg
     1021 tcatttcgat ggcctcctgt cgaaaacaat aggccttcga tgctaagttc tgtataccaa
     1081 caatacccgt ttcatcgcgg cactctggcg acactcctta aaaatcatat tcgtgctcac
     1141 ctttccttcc cgttcttcct tggtagcaaa ccggtaatac accgttcgcc agaccttacc
     1201 ttcgataacc agaagacctg cccgtgccat tttagctgcg gcctgattta tgctggttac
     1261 tgttgcgcct gttagcgcgg caacgtccgg cgcacagaag ctattatgcg tccccaggta
     1321 atgaataatt gcctctttgc ccgtcataca cttgctcctt tcagtccgaa cttagcttta
     1381 atttctgcga tcttcgccag cgcctgaaca cgatttagag gtctgccgcc catgacagga
     1441 agttgtttta ctggttcagg tatcgcctca ccacggttaa ttcgcgctgt catacaggtc
     1501 agttcatcgg cagccttgcg tcgtaattcc gcgtcagtca gcgcattggc ccgcatgttc
     1561 tggtacaggt tggtaaccag ccagtagtgc gcgttcgatt tccacggata agactctgca
     1621 tccggataca ggcctcgctt ccggcaatac tcgtaaacca tatcaaccag ctcgctgacg
     1681 tttggcagtc cggcgataac ggatgcttct tcccggcacc atgcaacaaa ctgcccgggt
     1741 gatggaagaa atggtcgatt ctgccgacgg gctacgcgca ttcctgcgtt aacctgttcc
     1801 atcgaggtga tcccgttttc ccggaaagcc agaacccact ggcggcggat ttcgttcact
     1861 tcgttctggt cacggttagc caggctcgcc gggaaagttg ccagtaactg gctgaacaca
     1921 ccgttgatga tctgcgctac ctgctgtacc tgcggctttt cgtcgtactg ttccggcatg
     1981 ttgttggcga tccgacgcat ctgctcacgg tcaaagttaa ccatctgtgc ggcgatgttt
     2041 ttcatagatc caccccgtaa atccagtctg tgtttgtcag gtcgagtttt ggtttgctgg
     2101 ctgtcacgcc tgcctgttgc ttgttacggt tgatttcgag ctgggtccac ttatcgcgga
     2161 gtttggccgg gctcagcacg ttaccggacc agaagttgtc ctggcatgcc cagcggaaca
     2221 gcacgcacat gtcgcggtgg ttacgtccgt cacgttcacg catcaggcgg atatcgttag
     2281 cccaccctgc aaaattcggt tttctggctg atggcgcgat ggtcttcacc atgtcaaaca
     2341 tccactctgc ggcggtcagg tcttctgctg tcccccactt gctgccgctc tgaatcgcag
     2401 catccgcttt caccacagga aggtcgtttt ctggcaggtc agaggattcg ccagaattct
     2461 cggacgaata aggttttata ttgtcttttg ttagtttgtc ttttgtgttt acctgattcg
     2521 ggtaagtgcc tttacctgat ttgggtaaac ttttcttacc tgattcaggt aaatttacct
     2581 ctttcaggta aactttattt ttcttacctg attcgggtaa tgttgaccat tcactgacca
     2641 cattattaat gccgatattc cgcccgctct gaataaaaat cccacgcttt accagaacac
     2701 tttttgcagc agaacacttg tgcggcaata tcccggtcaa ctcggaaagt tgctcgttgc
     2761 tcacccagtc cagtttttta ttaaagccat atgttttgcg catgacagcc aggaagacca
     2821 gaagctggtg ctgtgttaat ccggccagca ttacagcttc cagcaactca tttgcaatgc
     2881 gcgtataacc atcatcgaga tctgccacgc gcggctcctt ttgtgccgca tccggcactg
     2941 gaaaattgaa tatctcagca gtgtttgcca taattcctcc cgcaatgagt gtgttacgat
     3001 ttgcacctga aagtcggttc tgttccagca gaccggcttt cgccatttct gaacctgtca
     3061 tatcgccccc agcatggtag taaccatcgc catcaatgga ccagccagct ctgggtccac
     3121 acgaaacatc gacacaatac cttcactaat ttccttcagt ttctggtggc gtggtgcgtt
     3181 gagaatgaca gcctgttttg cctcactgag ttccttttcc atttcagcca acctagccat
     3241 gaagctatcc tgctcaacca ggtaaccgcg atattccagc ggtagtaccg ccagaattgc
     3301 cggggtcagt tcacgcacgt tatttcggta tttttcagaa tcgaatttgt tatcgaggaa
     3361 gcggaacagc ttctggcgtg tacggctgac atcatcaggg aaatcgatgg tgccgccgcc
     3421 ctgctcccga tactcattca caatgagtgt ggcaacgaca tcctgattat ctacagccga
     3481 ccaggcgcgg acggcatcac ggattttttc gtggcctggc acctgtttta tttgagaacg
     3541 atttatcacc gcagtcgggc taaatccgct agtctgttgg tatgtaagtg gttgcataat
     3601 cattgcctta tcagttaacg ccgcagttta ggcggcagaa ttactcgcgt taaacaatgg
     3661 tgcaaggtcg ggacgaatat ctgctggttt aatctttcca ccagtggctg agacaatttt
     3721 cattacatag cgggcatcaa ttccgccacc gtgtagccaa cgccaaacag tgggctacac
     3781 cgcatagatc tgccagtcgt ttttgactac ctgtaatact gattgcgagt tgaatggttt
     3841 gatttgtcat tatcaattcc tattggtatt acaatgaatg gataatagca atgcgtatta
     3901 atccaatcaa tagcaaaacg tgttttgacc atcaatacgc aagcgtataa attaaaactt
     3961 atgaaaaaag aaactcttgc tgatcgctta aacctagcga tggaacaatc tggaatgtct
     4021 caaggcgctc ttgcaaaggc gtctggcgta gctcaaccca caatctggag actgacaagc
     4081 ggcaacgcac gcggctcaac aaaaattgtt gaaatagcta atgcattggg tgttcgaaca
     4141 gagtggctct catcaggcat aggcccgatg agaaatgacg gtcaacaatc agggaagcct
     4201 gctgtcaacc attccaaata cttcaagatt gacgttcttg atatagaagt cagtgctggg
     4261 ccgggtgtca tcaaccgtga gtttgtagaa gtcctacgct cggttgagta ctcgtttgac
     4321 gatgctcgtc acatgttcga tggcaggaag gcagaaaata tccgcatcat taacgtacgc
     4381 ggtgacagca tgtcaggaac gatcgaacca ggtgatcttc tgttcgttga tatcactgtt
     4441 aaatctttcg acggtgatgg tatctatgcg tttctgtacg acgacacagc ccatgtaaag
     4501 cgcctgcaaa tgatgaagga taagctgctg gttatctctg ataacaagag ctactcaccg
     4561 tgggacccga tcgaaaaaga cgaaatgaat cgggtattta tatttgggaa agttattggg
     4621 agcatgccac agacatatag aaaacatggg tagattatta atgacagatt tcatcaagat
     4681 aaccagatga tgttttggtg agacatgctg acatcctcat aatttataga atggatgttt
     4741 caagcagagc ttaaggtata tggatatgtc aaaaaaaaat tttgaattca aaaattaccc
     4801 aatagtcttt attggctcag gcatttcaaa aagatattta gagaactacc caacgtggga
     4861 agagctatta aacgagtatt gga
//
LOCUS       DAZFBR010000208         4791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFBR010000208 DAZFBR010000000
VERSION     DAZFBR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.208"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1268
                     /gene="garP"
                     /locus_tag="ACHJZ2_004791"
     CDS             <1..1268
                     /gene="garP"
                     /locus_tag="ACHJZ2_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO4570742.1"
                     /translation="FIVTAVNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYL
                     LMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFS
                     EAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTV
                     MGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLH
                     YIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCG
                     FAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALA
                     FFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAA
                     LVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1284..2054
                     /gene="garL"
                     /locus_tag="ACHJZ2_004792"
     CDS             1284..2054
                     /gene="garL"
                     /locus_tag="ACHJZ2_004792"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO4570743.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2084..2974
                     /gene="garR"
                     /locus_tag="ACHJZ2_004793"
     CDS             2084..2974
                     /gene="garR"
                     /locus_tag="ACHJZ2_004793"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO4570744.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3071..4216
                     /gene="garK"
                     /locus_tag="ACHJZ2_004794"
     CDS             3071..4216
                     /gene="garK"
                     /locus_tag="ACHJZ2_004794"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO4570745.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4249..4625
                     /gene="rnpB"
                     /locus_tag="ACHJZ2_004795"
     ncRNA           4249..4625
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHJZ2_004795"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1108 a         1145 c         1382 g         1156 t
ORIGIN      
        1 tttttattgt taccgccgtt aactacgccg atcgtgcaac gctgtctatt gctggtaccg
       61 aagtggcaaa agagttgcag ttaagtgcgg tttcgatggg ttacatcttt tctgcttttg
      121 gctgggccta cttgctgatg caaatccccg gcggctggct gcttgataag tttggctcga
      181 aaaaagttta cacctacagc ctctttttct ggtcgctatt caccttcctg caaggctttg
      241 ttgatatgtt cccgctggcc tgggcaggga tctccatgtt ctttatgcgc tttatgctcg
      301 gcttctcgga agcgccatca ttcccggcga acgcccgtat tgtcgccgcc tggttcccga
      361 cgaaagaacg tggtactgcc tccgccatct ttaactcggc gcaatatttc tcgctggcgc
      421 tcttttcgcc tctgcttggc tggctgactt tcgcctgggg ctgggagcac gtctttaccg
      481 ttatgggggt gattggtttt gtgctgacgg cgctgtggat caaattgatt cataacccga
      541 cagatcaccc acgtatgtct gcggaagagc tgaagtttat ctctgaaaat ggcgcggtgg
      601 tcgatatgga ccacaaaaag ccgggcagtg cggcagcaag cggacccaaa ctgcattaca
      661 tcaagcaatt gctctctaac cgcatgatgt tgggcgtatt tttcggacaa tattttatca
      721 acaccatcac ctggttcttc ctcacctggt tcccgattta tctggtgcag gaaaaaggta
      781 tgtcgattct gaaagtgggt ctggtcgcct cgattccagc actgtgtggt tttgcgggcg
      841 gcgtgctggg aggtgtcttc tcggattatc tgatcaaacg cggtttatcc ctgaccctgg
      901 cacgtaagct accgattgtg ctgggaatgt tgctggcttc caccatcatc ttatgtaact
      961 acaccaacaa caccacgctg gtggtcatgc tgatggcgct ggctttcttt ggtaaaggat
     1021 ttggtgcgct gggctggccg gtgatttctg acaccgcgcc taaagagatt gttggcctct
     1081 gcggcggcgt ctttaacgtc tttggcaatg ttgcctccat tgtcactcca ctggtgattg
     1141 gctacctggt aagtgaactg cactccttca atgcagcact ggttttcgtg ggatgttcag
     1201 cgctaatggc gatggtctgc tacctcttcg tagttggcga cattaaacgt atggaattgc
     1261 agaaataagc aaaggtataa gcgatgaata acgatgtttt cccgaataaa ttcaaagccg
     1321 cactggctgc gaaacaggta caaattggtt gctggtcagc actctctaac ccgattagca
     1381 ctgaagttct tggtttggct gggtttgact ggctggtgct ggatggcgaa catgcgccaa
     1441 acgatatttc aacgtttatt ccgcagttaa tggccctgaa aggcagcgcc agcgcgccag
     1501 tagtgcgagt gccgaccaac gagccggtaa ttattaagcg tcttctggat atcggtttct
     1561 ataacttcct gattcctttt gtagaaacaa aagaggaagc agagcaggcg gtggcatcga
     1621 cgcgttatcc gccagaaggc attcgcggcg tctccgtttc tcaccgcgcc aatatgtttg
     1681 gcaccgtggc ggattatttc gctcagtcga acaagaacat cactattctg gtccagatag
     1741 aaagtcagca gggcgtagat aacgtcgatg ctattgccgc taccgaaggc gtagacggca
     1801 tcttcgtcgg ccccagcgat ctggccgcgg cattaggcca tctcggcaat gcatcacacc
     1861 cggatgtaca aaaagcaatt cagcacattt ttaaccgtgc cagcgcgcac ggcaaaccca
     1921 gcggtatcct cgcgccggtc gaagccgatg cgcgtcgtta tctggaatgg ggcgcgacgt
     1981 ttgtggctgt cggcagcgat ctcggcgtct tccgctctgc cactcagaaa ctggctgata
     2041 cctttaaaaa ataaccacca ccgcaacaag agagatgatt gatatgacta tgaaagttgg
     2101 ttttattggc ctggggatta tgggtaaacc aatgagtaaa aaccttctga aagcaggtta
     2161 ctcgctggtg gttgctgacc gtaacccaga agctattgct gacgtgattg ctgcaggtgc
     2221 agaaacagct tctacggcta aagcgatcgc tgaacagtgc gacgtcatca taaccatgct
     2281 gccaaactcc cctcatgtga aagaggtggc gctgggtgag aatggcatta ttgaaggcgc
     2341 gaagccaggt acggtattga tcgatatgag ttctatcgca ccgctggcaa gccgtgaaat
     2401 cagcgaagcg ctgaaagcga aaggcattga tatgctggat gctccggtga gcggcggtga
     2461 accgaaagcc atcgacggta cgctgtcagt gatggtaggc ggcgacaagg ctattttcga
     2521 caaatactat gatttgatga aagcgatggc gggttccgtg gtgcataccg gggaaatcgg
     2581 tgcaggtaac gtcaccaaac tggcaaatca ggtcattgtg gcgctgaata ttgccgcgat
     2641 gtcagaagcg ttaacgctgg caactaaagc gggcgttaac ccggacctgg tttatcaggc
     2701 aattcgcggt ggactggcgg gcagtaccgt gctggatgcc aaagcgccga tggtgatgga
     2761 ccgcaacttc aagccgggct tccgtattga tctgcatatt aaggatctgg cgaatgcgct
     2821 ggatacttct cacggcgtcg gcgcacaact gccgctcaca gctgcggtta tggagatgat
     2881 gcaggcactg cgtgcagatg gtttaggaac ggcggatcat agcgccctgg cgtgctacta
     2941 cgaaaaactg gcgaaagtcg aagttactcg ttaatgacgt cgtgcccgga tggcgtattg
     3001 caatccgggc ctggaatcca ggccgaataa gagaaacgcc ctccggcgtc atgtggtaac
     3061 aggcataggt atgaaaatcg taatcgcccc agactcttat aaagaaagtt tatctgccag
     3121 cgaggttgcg caggcgatag aaaaaggatt tcgggaaatt tttcctgatg cacagtacgt
     3181 ttctgttcct gttgccgacg gtggcgaagg aacggtggaa gcgatgattg cagccaccca
     3241 gggttccgaa cgtcacgcct gggttacagg gccgctgggc gagaaagtga atgccagttg
     3301 ggggatctcc ggcgatggca aaaccgcgtt tattgaaatg gcggcggcca gtgggctgga
     3361 gctggtacct gcggaaaaac gcgatccact cgtgaccact tcacgcggca caggcgagtt
     3421 aatcctgcag gcgctggaga gcggcgcgac aaacattatt atcggcattg gcggcagcgc
     3481 tacaaatgat ggcggcgcag gcatggtaca ggcgctgggg gcgaaattat gcgacgccaa
     3541 cggcaatgaa attggttttg gcggcggtag tcttaatact ctgaatgata ttgatatttc
     3601 cggcctcgat ccgcgcttaa aagattgcgt cattcgcgtc gcttgtgatg tcaccaatcc
     3661 gctggtgggc gataacggcg catcgcgcat ctttggccca caaaagggag ccagtgaagc
     3721 gatgattgtt gagctggaca ataacctctc tcactatgcc gaggtcatta aaaaagcgct
     3781 gcatgttgat gtgaaagatg tccccggcgc aggagctgcg ggtggtatgg gcgcggcgct
     3841 aatggcgttt cttggtgcgg aactgaaaag tggtattgaa atcgtcacta cggcgctgaa
     3901 tctggaggaa catattcacg attgtacgct ggtgatcacc ggtgaagggc gtattgacag
     3961 ccagagtatt cacggcaagg taccgattgg tgtcgcaaac gtggcgaaga agtaccataa
     4021 accggtgatt ggcattgcgg gtagcctgac cgatgatgtt ggcgttgtac atcagcatgg
     4081 cattgatgcg gtcttcagcg tattgaccag cataggtacg ttggacgaag cattccgcgg
     4141 ggcttatgac aatatctgcc gtgcttcacg taatatcgcc gcgacactgg cgattggaat
     4201 gcgcaacgcg gggtgacaag ggcgcgcaaa ccctctatac tgcgcgccga agctgaccag
     4261 acagtcgccg cttcgtcgtc gtcctcttcg ggggagacgg gcggagggga ggaaagtccg
     4321 ggctccatag ggcagggtgc caggtaacgc ctggggggga aacccacgac cagtgcaaca
     4381 gagagcaaac cgccgatggc ccgcgcaagc gggatcaggt aagggtgaaa gggtgcggta
     4441 agagcgcacc gcgcggctgg taacagtccg tggcacggta aactccaccc ggagcaaggc
     4501 caaatagggg ttcataaggt acggcccgta ctgaacccgg gtaggctgct tgagccagtg
     4561 agcgattgct ggcctagatg aatgactgtc cacgacagaa cccggcttat cggtcagttt
     4621 cacctgattt acgtaaaaac ccgcttcggc gggtttttgc ttttggtgca cagggtaaga
     4681 tgaataaata tctacggtat taataataga ccttgtaaga agttattaag tctatattgc
     4741 atttaaaagg tcgtaattat acgtatagtt ctctctggta aaatattgat t
//
LOCUS       DAZFBR010000209         4790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFBR010000209 DAZFBR010000000
VERSION     DAZFBR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.209"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..359
                     /gene="cesT"
                     /locus_tag="ACHJZ2_004796"
     CDS             <1..359
                     /gene="cesT"
                     /locus_tag="ACHJZ2_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFO4570746.1"
                     /translation="ISLSDANDEYMMIYGVCGKFPTDNSNFALEILNANLWFAENGGP
                     YLCYEAGAQSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMK
                     IEEISSSDNKHYYAGR"
     gene            419..3223
                     /gene="eae"
                     /locus_tag="ACHJZ2_004797"
     CDS             419..3223
                     /gene="eae"
                     /locus_tag="ACHJZ2_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFO4570747.1"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDQTKASITEIKADKTTAVANGKDAIKYTV
                     KVMKNGQPVNNQSVTFSTNFGMFNGKSQTQATTGNDGRATITLTSSSAGKATVSATVS
                     DGAEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQYGQFKLKASGGDGTYSWYS
                     ENTSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPSYMIKVDKQAYYADAMS
                     ICKNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWIKQTSSEQRSGVSSTYNLIT
                     QNPLPGVNVNTPNVYAVCVE"
     gene            complement(3487..4707)
                     /gene="escD"
                     /locus_tag="ACHJZ2_004798"
     CDS             complement(3487..4707)
                     /gene="escD"
                     /locus_tag="ACHJZ2_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO4570748.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DVLTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNAKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
BASE COUNT         1425 a          919 c         1033 g         1413 t
ORIGIN      
        1 atatttcgtt atctgatgcc aatgacgaat atatgatgat ttatggtgtc tgtgggaaat
       61 tcccgacaga taactctaac ttcgctcttg agattttgaa tgcaaactta tggtttgcag
      121 agaatggtgg gccatatctg tgctatgagg ctggagcaca atcgctgttg ttagcgttac
      181 gtttccctct cgatgatgct acccctgaaa aactcgagaa tgaaatagaa gtcgttgtta
      241 agtcaatgga aaacctgtat ttggtattac ataatcaggg aataacatta gaaaacgaac
      301 atatgaaaat agaggaaatc agttcaagcg acaataaaca ttattacgcc ggaagataaa
      361 atccgatcta ttaatataat ttatttctca ttctaactca ttgtggtgga gccataacat
      421 gattactcat ggttgttata cccggacccg gcacaagcat aagctaaaaa aaacattgat
      481 tatgcttagt gctggtttag gattgttttt ttatgttaat cagaattcat ttgcaaatgg
      541 tgaaaattat tttaaattgg gttcggattc aaaactgtta actcatgata gctatcagaa
      601 tcgccttttt tatacgttga aaactggtga aactgttgcc gatctttcta aatcgcaaga
      661 tattaattta tcgacgattt ggtcgttgaa taagcattta tacagttctg aaagcgaaat
      721 gatgaaggcc gcgcctggtc agcagatcat tttgccactc aaaaaacttc cctttgaata
      781 cagtgcacta ccacttttag gttcggcacc tcttgttgct gcaggtggtg ttgctggtca
      841 cacgaataaa ctgactaaaa tgtccccgga cgtgaccaaa agcaacatga ccgatgacaa
      901 ggcattaaat tatgcggcac aacaggcggc gagtctcggt agccagcttc agtcgcgatc
      961 tctgaacggc gattacgcga aagataccgc tcttggtatc gctggtaacc aggcttcgtc
     1021 acagttgcag gcctggttac aacattatgg aacggcagag gttaatctgc agagtggtaa
     1081 taactttgac ggtagttcac tggacttctt attaccgttc tatgattccg aaaaaatgct
     1141 ggcatttggt caggtcggag cgcgttacat tgactcccgc tttacggcaa atttaggtgc
     1201 gggtcagcgt tttttccttc ctgcaaacat gttgggctat aacgtcttca ttgatcagga
     1261 tttttctggt gataataccc gtttaggtat tggtggcgaa tactggcgag actatttcaa
     1321 aagtagcgtt aacggctatt tccgcatgag cggctggcat gagtcataca ataagaaaga
     1381 ctatgatgag cgcccagcaa atggcttcga tatccgtttt aatggctatc taccgtcata
     1441 tccggcatta ggcgccaagc tgatatatga gcagtattat ggtgataatg ttgctttgtt
     1501 taattctgat aagctgcagt cgaatcctgg tgcggcgacc gttggtgtaa actatactcc
     1561 gattcctctg gtgacgatgg ggatcgatta ccgtcatggt acgggtaatg aaaatgatct
     1621 cctttactca atgcagttcc gttatcagtt tgataaatcg tggtctcagc aaattgaacc
     1681 acagtatgtt aacgagttaa gaacattatc aggcagccgt tacgatctgg ttcagcgtaa
     1741 taacaatatt attctggaat acaagaagca ggatattctt tctctgaata ttccgcatga
     1801 tattaatggt actgaacaca gtacgcagaa gattcagttg atcgttaaga gcaaatacgg
     1861 tctggatcgt atcgtctggg atgatagtgc attacgcagt cagggcggtc agattcagca
     1921 tagcggaagc caaagcgcac aagactacca ggctattttg cctgcttatg tgcaaggtgg
     1981 cagcaatatt tataaagtga cggctcgcgc ctatgaccgt aatggcaata gctctaacaa
     2041 tgtacagctt actattaccg ttctgtcgaa tggtcaagtt gtcgaccagg ttggggtaac
     2101 ggactttacg gcggataaga cttcggctaa agcggataac gccgatacca ttacttatac
     2161 cgcgacggtg aaaaagaatg gggtagctca ggctaatgtc cctgtttcat ttaatattgt
     2221 ttcaggaact gcaactcttg gggcaaatag tgccaaaacg gatgctaacg gtaaggcaac
     2281 cgtaacgttg aagtcgagta cgccaggaca ggtcgtcgtg tctgctaaaa ccgcggagat
     2341 gacttcagca cttaatgcca gtgcggttat attttttgat caaaccaagg ccagcattac
     2401 tgagattaag gctgataaga caactgcagt agcaaatggt aaggatgcta ttaaatatac
     2461 tgtaaaagtt atgaaaaacg gtcagccagt taataatcaa tccgttacat tctcaacaaa
     2521 ctttgggatg ttcaacggta agtctcaaac gcaagcaacc acgggaaatg atggtcgtgc
     2581 gacgataaca ctaacttcca gttccgccgg taaagcgact gttagtgcga cagtcagtga
     2641 tggggctgag gttaaagcga ctgaggtcac tttttttgat gaactgaaaa ttgacaacaa
     2701 ggttgatatt attggtaaca atgtcagagg cgagttgcct aatatttggc tgcaatatgg
     2761 tcagtttaaa ctgaaagcaa gcggtggtga tggtacatat tcatggtatt cagaaaatac
     2821 cagtatcgcg actgtcgatg catcagggaa agtcactttg aatggtaaag gcagtgtcgt
     2881 aattaaagcc acatctggtg ataagcaaac agtaagttac actataaaag caccgtcgta
     2941 tatgataaaa gtggataagc aagcctatta tgctgatgct atgtccattt gcaaaaattt
     3001 attaccatcc acacagacgg tattgtcaga tatttatgac tcatgggggg ctgcaaataa
     3061 atatagccat tatagttcta tgaactcaat aactgcttgg attaaacaga catctagtga
     3121 gcagcgttct ggagtatcaa gcacttataa cctaataaca caaaaccctc ttcctggggt
     3181 taatgttaat actccaaatg tctatgcggt ttgtgtagaa taattccata accaccccgg
     3241 ctaaaatatg tattgtttta gtcggggcat aattatttct tcttaagaaa taacctctta
     3301 taatcaaatc tactactggt ctttttatct gcttaatagg tctctttcaa agagacacat
     3361 tcacgttttc tcatgtagct tgatccaacc acgctgatac caaagctgaa tcacatcaag
     3421 caacaactat gctcacaaca tccacacaat aaaaaaccct ccgaagaggg ggaagaggat
     3481 aagaaattaa tacgacagtg gaatatgtat atattcgtct gggcgggaga tgttgatacc
     3541 atctttagga tcaatcgcgt ctatgacata gcctcctttc agaatgctac caatccccag
     3601 gctggtgcca ttatcaagag caattgttgg attcttcatc gtaccgctga ttctgagtat
     3661 ttttccgggg aaaatatctg ctgaaatgta cggctgaatg ttctgaaaga gtatcttggc
     3721 gttactattt ttgttcattg catttataat ggcaaggatc ttactctcat tctgttgaga
     3781 aacttccccc gcaacaatga tgacatcatt ctttttgaaa atattgacat agtttattag
     3841 tttattcttt ttaaactctg atgccagttc attgatttta ttgttactgt tgttgtgtat
     3901 ttcccatcct gcaactcctg gcgtattcag taacagattt tcaacctcgc tccatttggg
     3961 tgaaggggga atgtatcccg ataacagaaa gaatccaggc ttattacctt ttgtaataat
     4021 gatatctttg taaccgtatt ccgttaagac atcattaatt gcggatatga tttggttatt
     4081 gcaaatgata ttattgtaat aaaaaatatt tttttctttt aggtaattaa aaaatggagt
     4141 taaatccgtg gttgattcgc aacgcccata aagcgcgata tttttcccat gccataccag
     4201 agtgattgca tgaagtttgt tttcttttag ctgctgatat acatcagctc tggtaaacgt
     4261 tgggggttca gcttttgggg aaagcaaaac gtaactgccg atggcaccag acaacgcaaa
     4321 agtagcacca atcacgctcg ccaggataag gcgttttttg cgtcgacttt tggcagagaa
     4381 cctttctggc acatcagaca atggcaagga atggtcaata tcaccaatga tgaagcaaca
     4441 tcccgctata tcaataacct ttccgacagg taatggttta tctgcttcat agggcgtggg
     4501 ttgcccatca atccaaaatt caaccggaga taaaaggaaa acaccttcct cacgtatatc
     4561 aagtaaaaac tgattgaggc cttgttcaag gggaaagtaa acaatatcgt tatcttccgt
     4621 acctagcgtt aggtttccca tcggaagttg taattcccga ttacgcatcg caccattgag
     4681 aagttttatt ttatatgagg ataacatttt taaattttat tcatcctggt ggttgaacaa
     4741 tgagaaaaaa tatggtgaac ttacatcgtc taagaataga gtagaaagga
//
LOCUS       DAZFBR010000210         4585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFBR010000210 DAZFBR010000000
VERSION     DAZFBR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.210"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            242..784
                     /gene="fimA"
                     /locus_tag="ACHJZ2_004799"
     CDS             242..784
                     /gene="fimA"
                     /locus_tag="ACHJZ2_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4570749.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            1004..1696
                     /gene="fimC"
                     /locus_tag="ACHJZ2_004800"
     CDS             1004..1696
                     /gene="fimC"
                     /locus_tag="ACHJZ2_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO4570750.1"
                     /translation="MMTKIKLLMLIIFYLIISASVHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1727..4336
                     /locus_tag="ACHJZ2_004801"
     CDS             1727..4336
                     /locus_tag="ACHJZ2_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO4570751.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4349..>4585
                     /gene="fimH"
                     /locus_tag="ACHJZ2_004802"
     CDS             4349..>4585
                     /gene="fimH"
                     /locus_tag="ACHJZ2_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="involved in the regulation of fimbriae length and
                     mediates adhesion of type 1 fimbriae; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HFO4570752.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTY"
BASE COUNT         1239 a          973 c         1074 g         1299 t
ORIGIN      
        1 ctgacaatat aaaaagaatt ttcagcctgg taatttaccg cttcaggtct atatttgtgt
       61 tgaatatatt ttgcgcggaa gtattcatct aacggggctc tctatttttt agaatagagt
      121 gcatattttc aattaagaca ttcttagagg ataaaaagga atttactact atcagtgtct
      181 taaataaagt aatcggttat atacggatgt ggagtcgata aatgagattg aaggaatata
      241 tatgaaatta agatttattt cgtctgcgct ggctgccgca ctattcgccg ctacgggtag
      301 ttatgctgcc gttgtagatg gcggtacaat tcactttgaa ggtgaactgg tgaatgctgc
      361 ctgttcagtg aatactgact cggcagacca ggttgtcaca ctcggtcaat atcgtaccga
      421 tattttcaat gctgttggta atacctctgc attaattcca ttcaccattc agttgaacga
      481 ctgcgatcct gttgttgccg ctaatgctgc cgttgcattt tctggtcagg ctgatgcaat
      541 caatgataat ttattggcca ttgcatccag taccaataca acaacagcaa cgggtgtcgg
      601 tattgaaata cttgataata catccgcaat tctcaaacct gatgggaata gcttctcaac
      661 caaccagaac ttgatccccg ggaccaacgt tcttcatttt tctgcacgtt ataaaggcac
      721 cggtacaagt gcatcagcag ggcaagcaaa tgctgacgcg acttttatta tgagatacga
      781 ataatcaaaa ccacgttgtt ttgaattata tatcacgtct tataacaaag taatgtaccg
      841 gttgtctgaa gcggtatggt ggcaatgtaa atcgaaatca tgttcacttt gtatcatgcc
      901 gctttattaa atgaaaaggg aatgatgtgt tgtaagaaac caaagcaatc atttctttat
      961 atttcttatt tttgccgtca ggaatacaca aggcgtatta actatgatga ctaaaataaa
     1021 gttattgatg ctcattatat tttatttaat catttcggcc agcgtccatg ctgccggagg
     1081 gatcgcatta ggtgccacgc gtattattta tcccgctgat gctaaacaga ctgcggtatg
     1141 gattagaaat agccatacca atgagcgttt tctggtcaat tcgtggattg aaaacagcag
     1201 cggtgtaaaa gaaaagtcat tcatcattac accgccacta tttgttagtg aacccaaaag
     1261 cgaaaatact ttgcgtatta tttacaccgg tccaccgctg gcagcagatc gtgagtctct
     1321 gttctggatg aatgttaaga cgattccttc ggtagataaa aatgcattga acggcaggaa
     1381 tgttttgcaa ctggcgattt tatcgcgcat gaagttattt ctccgtccaa ttcaactaca
     1441 agaattaccc gcagaagcgc cggatacact caagttttcg cgatccggta actatatcaa
     1501 tgttcataat ccatcacctt tttatgtcac cctggttaac ttacaagtgg gcagccaaaa
     1561 gttggggaat gctatggctg cacccagagt taattcacaa attcccttac cctcaggagt
     1621 gcagggaaag ctgaaatttc agaccgttaa tgattatggt tcagtaaccc cggtcagaga
     1681 agtgaactta aactaaccga atcatctgac aatatcagag ctaattatga aaatacccac
     1741 tactacggat attccgcaga ggtatacctg gtgtctggcc ggaatttgtt attcatctct
     1801 tgccatttta ccctcctttt taagctatgc ggaaagttat ttcaacccgg catttttatt
     1861 agagaatggc acattcgttg ctgatttatc gcgctttgag agaggtaatc atcaacctgc
     1921 gggcgtgtat cgggtggatc tctggcgtaa tgatgagttc attggttcac aggatatcgt
     1981 atttgaatcg acaacagaaa atacaggtga taaatcaggt gggttaatgc cctgttttaa
     2041 ccaggtactc cttgaacgaa ttggccttaa tagcagtgca tttcccgagt tagcccagca
     2101 gcaaaacaat aaatgcatca atttactgaa agctgtacct gatgccacaa ttaactttga
     2161 ttttgcagcg atgcgcctga acatcactat tcctcagata gcgttgttga gtagcgctca
     2221 cggttacatt ccgcctgaag agtgggatga aggtattcct gctttactcc tgaattataa
     2281 tttcaccggt aacagaggta atggtaacga tagctatttt tttagtgagc tcagcgggat
     2341 taatattggc ccgtggcgtt tacgcaacaa tggttcctgg aactattttc gcggaaatgg
     2401 atatcattca gaacagtgga ataatattgg cacctgggta cagcgcgcca ttattccgct
     2461 gaaaagtgaa ctggtaatgg gagacggcaa tacaggaagt gatattttcg atggtgttgg
     2521 atttcgtggt gtacggcttt attcttccga taatatgtat cctgatagcc agcaagggtt
     2581 tgccccaacg gtacgtggga ttgcccgtac ggcggcccag ctaacgattc ggcaaaatgg
     2641 ttttattatc tatcaaagct atgtttcccc cggcgctttt gaaattacag atttgcaccc
     2701 gacatcttca aatggcgatc tggacgtcac catcgacgaa cgcgatggca atcagcagaa
     2761 ttacactatt ccgtattcaa cagtgccaat tttacaacgc gaagggcgtt tcaaatttga
     2821 cctgacggcg ggcgattttc gtagcggtaa tagtcagcaa tcatcgcctt tcttttttca
     2881 gggcacggca ctcggcggtt taccacagga atttactgcc tacggcggga cgcaattatc
     2941 tgccaattac accgcctttt tattagggct ggggcgcaac ctcgggaact ggggcgcagt
     3001 gtcgctggat gtgacccatg cgcgcagtca gttagccgac gacagtcgtc atgaggggga
     3061 ttccattcgc ttcctctatg cgaaatcgat gaacaccttc ggcaccaatt ttcagttaat
     3121 gggttaccgc tattcgacac aaggttttta tacccttgat gatgttgcgt atcgtcgaat
     3181 ggaggggtac gaatatgatt acgattatga cggtgagcat cgggatgaac cgataatcgt
     3241 gaattaccac aatttacgct ttagccgtaa agaccgtttg cagttaaata tttcacaatc
     3301 acttaatgac tttggctcgc tttatatctc tggtacccat caaaaatact ggaatacttc
     3361 ggattcagat acctggtatc aggtggggta taccagcagc tgggttggca tcagttattc
     3421 actctcattt tcgtggaatg aatctgtagg gatccccgat aacgaacgta ttgtcggact
     3481 taatgtttca gtgcctttca atgttctgac caaacgtcgc tacacccggg aaaatgcgct
     3541 cgaccgcgct tatgcctcct ttaacgccaa ccgtaacagc aacgggcaaa atagctggct
     3601 ggcaggtgta ggtgggacct tactggaagg ccacaacctg agttatcacg taagccaggg
     3661 tgatacctcg aataatgggt atacgggcag cgccacggca aactggcagg ccgcttacgg
     3721 tacgctgggg gtcgggtata actacgaccg cgatcaacat gacgttaact ggcagctgtc
     3781 tggcggtgtg gtcggacatg aaaatggcat aacgctgagc cagcctttag gggataccaa
     3841 tgttttgatt aaagcgcctg gcgcaggcgg tgtacgcatt gaaaatcaaa ctggcatttt
     3901 aaccgactgg cgcggctatg cggtgatgcc gtatgccacg gtttatcggt ataaccgtat
     3961 cgcgcttgat accaatacga tggggaattc catcgatgtt gaaaaaaata ttagcagcgt
     4021 tgtgccgacg caaggcgcgt tggttcgtgc caattttgat acccgcatag gcgtgcgggc
     4081 gctcattacc gttacccagg gcggaaaacc ggtgccgttt ggatcactgg tacgggaaaa
     4141 cagtaccgga ataaccagta tggtgggtga tgacgggcaa gtttatttaa gtggtgcgcc
     4201 attgtctggt gaattactgg ttcagtgggg agacggcgcg aactcacgct gcattgcgca
     4261 ctatgtattg ccgaagcaaa gcttacagca agccgtcact gttatttcgg cagtttgcac
     4321 acatcctggc tcataaagga aattatcaat gaagataatc tgcagattat tattggcgat
     4381 ggcatgtttg tgtctggcaa acatatcctg ggctactgtt tgtgcaaata gtactggcgt
     4441 agcagaagat gaacactatg atctctcaaa tgtctttaat agcaccaata accagccagg
     4501 gcagattgtt gttttaccgg aaaaatccgg ctgggtaggt gtctcagcaa tttgtccacc
     4561 cggcacgctg gtgaattata catac
//
LOCUS       DAZFBR010000211         4517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFBR010000211 DAZFBR010000000
VERSION     DAZFBR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.211"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1356
                     /locus_tag="ACHJZ2_004803"
     CDS             <1..1356
                     /locus_tag="ACHJZ2_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4570753.1"
                     /translation="ALTKTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQR
                     YISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKY
                     QTAQGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNENAAARASNDAELLG
                     YGQGERARERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLK
                     DQAAFYAESDAQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLN
                     GNKVEWRSWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANA
                     KGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVG
                     SMNGSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLS
                     G"
     gene            1349..1690
                     /locus_tag="ACHJZ2_004804"
     CDS             1349..1690
                     /locus_tag="ACHJZ2_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4570754.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDEATRCWLEEFLSWHGGYRAFLWRPPKHNRMVRVVCREWSVTDNVRYS
                     DFSCTIEQVVN"
     gene            1690..2388
                     /locus_tag="ACHJZ2_004805"
     CDS             1690..2388
                     /locus_tag="ACHJZ2_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4570755.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            2394..3137
                     /locus_tag="ACHJZ2_004806"
     CDS             2394..3137
                     /locus_tag="ACHJZ2_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4570756.1"
                     /translation="MTQTESAILAHARRCVPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            3134..3715
                     /locus_tag="ACHJZ2_004807"
     CDS             3134..3715
                     /locus_tag="ACHJZ2_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4570757.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQA"
     gene            3951..>4517
                     /locus_tag="ACHJZ2_004808"
     CDS             3951..>4517
                     /locus_tag="ACHJZ2_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4570758.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPERPFNIRMVRETADST"
BASE COUNT         1028 a         1075 c         1516 g          898 t
ORIGIN      
        1 gccctgacaa aaacagaacg tgagctggcc ctgtcccgac tgaaggggga ggaaaaagag
       61 cgtgcccggc tggggtatgc ggcggatgac cttggttttg tgggggattc gtatcaggag
      121 gcgagacagc gttatatcag taatgccctg gaagcctggc gtaataacga ggcgaacaaa
      181 cccaaatccc ggggtggaaa atcagagacg gaaaaagcgg aagacagttt ttcccggcta
      241 ctgaagcagc agaaagagca actggcactg gcgggtcaga atacagagct ggcgaagctg
      301 aagtaccaga ctgcgcaggg cgaactgaaa accctgacgg agatgcagaa gcaggagctg
      361 ctgcgtaatg cggccctgat tgaccagcaa aaaatccggg aacagttgcg atcccgggaa
      421 gagacactga agaatgagaa tgcggctgcg cgtgcgtcga atgatgctga actgctgggg
      481 tacgggcagg gggaacgagc cagggaacgc atgcgggagt tgcagcagat ccgcgacagc
      541 ttccgccaga aggatgcgga ccttcagtct cagtatcaga ccggggatat cagtgaggat
      601 ttttacagac aggcacgggc acagaacgcg cagtatctga gcgaacgcct taaggaccag
      661 gcagcctttt atgccgaatc ggatgcgcag cgtgcggact ggcagaaagg gctgcaggag
      721 ggattcagta actgggtgga taatgcgtcc gattacgcct cacaggcagc acagcttgcg
      781 acggagggta tctcagggat ggtgaataac atcacggaga tgctgaacgg aaataaagtg
      841 gaatggcgca gctgggcctc atcagtgctg caggaaatat caaaagttct tatgaatgcc
      901 gcgattgtca acggaattaa gacggcggca aacggtatgt ccggtgcggg aggatttctc
      961 ggcagcattg gtgactggct gggcggagcg gtggccaatg caaaaggcgg cgtgtatacc
     1021 tcggcaaacc tgagtgcgta cagcaacagt attgtggata cgcccacgta ctttgccttt
     1081 gcaaaagggg ccgggctgat gggggaggcc ggacctgaag ccattatgcc tctgacgcgg
     1141 gcggcggatg gctcgctggg tgtgcgcgcg gtgggtagta tgaacggcag tgcgggtctg
     1201 gtgtattccc cggtctacca tatcgccatt cagaatgacg ggactaatgg ccagataggg
     1261 ccggaggcgg caggcagtct tgtgcagctg attgaccagc gggtgcaggc ggtgatgttg
     1321 tccatgcgac gtgacggagg aatgctgagt ggctgagata aaaacgctgc atctggtccc
     1381 gcgtgaaggg atgcaggtga gtgagaagcc gtcggtggtg agggtgcggt ttggtgacgg
     1441 ttatgaacag cgtcgcccca caggactgaa tcctcaactg aagacgtttc aggcggtgtt
     1501 ccgggtgacg gatgaggcga cccggtgctg gctggaagag tttttatcgt ggcatggtgg
     1561 ttaccgtgcc tttttatggc gaccgccgaa acataaccgg atggtcaggg tggtatgccg
     1621 tgagtggagc gtcacggata acgtccggta cagtgatttc agttgtacga ttgagcaggt
     1681 ggtgaactga tgcaggacat tcatgaagaa agcctgaacg agtcggttaa atcagagcag
     1741 tcaccgcggg tggtgctctg ggaaattgac ctgacggtgc agggcggtga gcggtatttt
     1801 ttctgtaatg agctgaatga aaaaggggag gcggtcacct ggcaggggcg gcaatatcag
     1861 gcatacccga ttgacggcag cggttttgag atgaacggga agggcagcag tgccagaccg
     1921 tcgctgacgg tgtccaatct gtttggtctg gtcaccggga tggcggagga cctgcagagc
     1981 ctggtggggg ccacggtggt ccgccgccgg gtgtatgccc gttttctgga tgcggtgaat
     2041 tttgtggcgg gcaatccgga agcggacccg gagcaggagc tgagcgaccg ctgggtggtg
     2101 gagcagatgt cagagctgac ggccatgaca gcctcgtttg tgctggcaac accgacggag
     2161 acggacggag cgctgtttcc cggtcgcatc atgctggcga acacctgtat gtgggattac
     2221 cggggagatg aatgcgggta taacggtcct gcggtggcgg atgagttcga caaccccacc
     2281 acggatatcc gtaaggacag atgcagcaag tgcatgcgcg ggtgtgagat gcgcggcatg
     2341 gtggctaatt ttggcggttt cctttccatt aacaaacttt cgcagtaaat ccaatgacac
     2401 agactgaatc agcgattctg gcgcatgccc ggcggtgtgt gcctgcggag tcgtgcggct
     2461 tcgtggtgag aacgccggag ggggagcggt atatcccttg tgtgaatatc tctgcagagc
     2521 cggaggcgta ttttcgtatt gcaccggaag actggctgcg ggcagagatg cagggggaga
     2581 ttgtggcact ggtccacagt catcccggtg gtctgccctg gctgagcgag gcagaccggc
     2641 gactgcagat aaaaagcgca ctgccctggt ggctggtctg ccggggtgac attcacaaat
     2701 tccgctgtgt gccgcacctg accggacggc gctttgagca cggggtgacg gactgttaca
     2761 ccctgttccg ggatgcatac catctggcgg gaattgatat gccggatttt gagcgtgagg
     2821 atgactggtg gcgcaacggt cagaaccttt acctggacaa tatggcggtc accggctttt
     2881 accgggtgcc cctgtcctct gcacaggcgg gcgatatcct gctgtgctgc tttggcgcat
     2941 cggtggccaa tcatgccgcc atttactgcg gcaacggtga actgcttcac catctgcctg
     3001 aacaactgag taaacgggag aggtattccg aaaaatggca acgacgaacg cattctgtct
     3061 ggcgtcaccg ccactggcac gcatctgcct tcacggggat ttacaacgat ttggccgccg
     3121 cctcagcctg tatgtgaaca cggcagcgga agccatccgg gcgctgtcgt tacaggtgcc
     3181 cggattccgc cgtcagatga acgaaggctg gtaccagata cgtattgccg gtgatgacac
     3241 ggcaccggag gcggtgtacg cccgtcttca cgaacagctg ggtgagggaa cggtcatcca
     3301 cattgtgccg cgactggccg gagccggaaa gggcggactg cagattgtgc tgggggcggc
     3361 agccatcgtg ggctctttct tcaccgccgg cgcaacgatg gcgttatggg gttcagccct
     3421 ggcagccggt ggtttttctg ccaccacgat gctgttttca cttggagcca gcatgatact
     3481 gggtggtgtg gcccagatgc tggccccgaa ggcaaaaaca ccggattacc gcgcaacgga
     3541 taacggcaga cagaacacgt atttttcgtc actggacaac atgattgccc agggtaaccc
     3601 gatgccggtg ccttacgggg aaatgctggt tggctcccgc cgtatatccc aggacatcag
     3661 tacccgtgat gaaggcgggg gcggaaaggt cgtggttatc gggcgacagg cataaaagcg
     3721 aaaaaatccc gcagtgaccg aaggctgcgg gaacagaaaa tgaagattaa ccacagggag
     3781 ttttgttttt attggcccga aaaaactgta acgcccggga atgatatctg ccacgggggc
     3841 gtacagaaaa tgtgaagata ttcagaattt ttattcagtc atgacacagg catcctccgg
     3901 gatgcctgtt gtttttgtgc gtaacagtta tcacagtaaa gggtgagaca atgggtaaag
     3961 gtggcggcaa ggcgcacacg ccggtagagg caaaggacaa tcttaagtcc acgcagatga
     4021 tgagcgtgat tgacgccatt ggtgaagggc cgattgaagg tccggtgaag gggttgcaga
     4081 gtatcctggt gaacaaaacc ccgctgacgg acacggacgg taatcctgtg atacatggtg
     4141 tgaccgcggt ctggcgcgcc ggggagcagg agcagacacc gccggaaggc tttgagtcct
     4201 ccggggcgga aaccgcactg ggcgtggaag tgacgaaggc aaagccggtg acgcgcacca
     4261 ttacgtccgc gaacattgac cgcctgcggg ttaccttcgg ggtgcagtca ctgttggaga
     4321 ccacctcaaa gggcgaccgt aatccctctt ctgtccgact gctgattcag ttgcagcgta
     4381 acggtaactg ggtgacggaa aaggatgtca ccattaacgg caagaccacc tcgcagtttc
     4441 tggcgtcggt gattctggat aatctgcctg agcggccctt taacatccgg atggtcaggg
     4501 agacggcgga cagcacc
//
LOCUS       DAZFBR010000212         4493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFBR010000212 DAZFBR010000000
VERSION     DAZFBR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.212"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..1069)
                     /locus_tag="ACHJZ2_004809"
     CDS             complement(<1..1069)
                     /locus_tag="ACHJZ2_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO4570759.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKSQNSNGLQYDALFIPSMAFSILVFSFN
                     GGIISKIISNKVMILLGDASFSFYLVHTIVISTLSKFFNVSGLGAISVIKFIVMALFA
                     SLFISIMMYLFFEKPINNKLRK"
     gene            complement(1079..1759)
                     /locus_tag="ACHJZ2_004810"
     CDS             complement(1079..1759)
                     /locus_tag="ACHJZ2_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO4570760.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            complement(1746..2213)
                     /locus_tag="ACHJZ2_004811"
     CDS             complement(1746..2213)
                     /locus_tag="ACHJZ2_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO4570761.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(2213..2749)
                     /locus_tag="ACHJZ2_004812"
                     /pseudo
     CDS             complement(2213..2749)
                     /locus_tag="ACHJZ2_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            2813..3460
                     /locus_tag="ACHJZ2_004813"
     CDS             2813..3460
                     /locus_tag="ACHJZ2_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4570762.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
     gene            complement(4130..>4493)
                     /locus_tag="ACHJZ2_004814"
     CDS             complement(4130..>4493)
                     /locus_tag="ACHJZ2_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570763.1"
                     /translation="SDLDKISGDIKRSYGVPSNQGIQGSFLIKCINSLTGLVGIPGLP
                     DKEFALSTPEFMKSKQHKAFSTVFKDVTNELTSIERLGGLRDKLASNFTIDDSYYTEP
                     KTEDPRFRYVSSNWKQPM"
BASE COUNT         1313 a         1011 c          967 g         1202 t
ORIGIN      
        1 attttcttag cttattgttt attggctttt caaaaaacaa atacatcatt attgatataa
       61 ataatgaagc aaacagagcc attactataa atttaattac acttatagca ccaagaccag
      121 aaacattaaa gaacttgctc aatgtactga ttactatagt atggaccaag taaaatgaaa
      181 aagaagcatc acctaaaagt atcataactt tatttgatat tatttttgaa ataataccac
      241 cattaaacga aaacactaat attgaaaagg ccattgatgg tataaaaagc gcatcatact
      301 gtaatccatt gctattttga ctttttgcca ccatatagac agacattagg ataaagacta
      361 cagatgatat ttcagcaaat gatagaacta aatatgacgg ctttgactta aactcaaaat
      421 ctccggcctg attgctgcgg attgtctctg ccagcctgcc tcggtcaatc tcacgaccag
      481 ccatcttgtc ctgaacattg aagtagtcaa tcggaccgag agcagccatc ccaaggtgat
      541 aaacaaactc accaaaccct gaaggattct gctgatacat ctgagcaacg ttgttagggt
      601 caacaccgac gcgcgccagt tccttggcgt tgttttgcag ccatgattgc attgcttctg
      661 gagacgaggc cgcaaggcgt gcgccagccg ctaaggtgcc gatagaattg cgctggtctt
      721 cgtctgccca cttcatacca gactgaatct tctctaattg accaggatat ttggtcatca
      781 gatctcgaac ctgctgtcga tcgccggact ggatggctgc cgcatattct ttttggaatg
      841 cagcatccgc ttcctgttgc tttgcggctt gagatgtttg agcgacacta ccaagtccct
      901 gcaacgcctg aaggccgatg ttattgcgac ctgaacgctc catttcgttg ttctggcgaa
      961 tataggccaa cgcctcactt acatcacttg cctttggcgc atttgagttt tgcccaccga
     1021 taccagcaag aaaaccgcct gagttgattc cttgttgcca agtagccata ttcccacctt
     1081 aaaacaatga tccaagacca ccgataacac cgccagcaag agcaccaata ccagtcccaa
     1141 taacaggaac tgcactacca atcattgcac ctgatgcggc cccaccaatg gcacctgtag
     1201 caaaactctg taggcctgaa ggcttattcg catttgctgc cgatgctgcc gcctgctgtt
     1261 gatacaattg gctgacattg ttagcgtagt tctgtccggc gtttgcctga cctgtaagag
     1321 caccaaggcc gatatttgcc agattgttgt agttgttcat ctgacctgac agccagtttt
     1381 gaccgagtgt aggtgcgatt gctgctaact ggtttcctgt tgctgtagag cctaatccac
     1441 ccgttgcctc tgctgctgcc agactctgat agcgcgcctg ccctgcaagg tctttatact
     1501 gctgagagtt gtaatactgg ttaagcgcct gaccttgccc ctgaagagag gaaagattct
     1561 gcaactgtga tacgtactgc tgagcgagtg gcgtgaacgg tgcaaggttt tgcatgttcg
     1621 tctgccacat ttcacgctgc agttcgatgc ccttttcagt tgcgcgtgcc tgggctttag
     1681 atccggagtc gctgccgcct ttcatatacc cattcatggg aagcaattta ttcttgaagc
     1741 tttcgctaag tactaacatt taatagctcc tcatatttcg aacgaggtaa ttgatagagg
     1801 gtgattccta ccggttttcc gttactcatg tacgcatcat caaggtgacc aacacgggta
     1861 gcgccaagca aacggataat tgcccgtccg tatttggtgg tgtcaggaac catggtgatg
     1921 ctgttaagga atggtgagtt ttcgagaagc catttgcaga ataatcgatg cccttgtagt
     1981 gcatattcgc cacggaatcc ggggtcatac accgcatggc attcaacaac gctatgccag
     2041 aagttacgca cttcatgaac tccagccagc actaatcctt cgtagatgcc gagatatacc
     2101 gcatcaggct tgatgtagta tttatctcca ctgtctacga tattccccgt gtttaccggg
     2161 ttgttaagga attctgcaag tttcaccggg ttatcgatga gctttatttc catcactgct
     2221 ccgcaatgat tttgatggtt gtggcagtaa acgccgcgcc attcgactga atggttaacg
     2281 tgctgccatt tgtggcaaga aagccgtctt tatccacgct gaagaacgta gctaacagga
     2341 tgttgtcggt tgttgttgcc gagttgcgac tgcttactag cgtgtcagga acagaaccgg
     2401 agaatgttag ctgcattgac ctgttggcgg ttccgctggg ccacgtcccg acaatcgaca
     2461 gcttgaagaa caaggttttg ttctcgttga acacaaccat cttgttgtta acggtgtcga
     2521 agaatggtgc caacgtcccg gatgacggcg taagcgtttt cagcaggcta acaaggttgg
     2581 tcggcgctgt cgggatggtt actgatacgc cagagtaaac aacctctgac ttcttgcgcg
     2641 tagtggcata ctccagagca ttaatgcgcg tttcatggtc tgaaacctgc gattccagcg
     2701 actgaactct ggtatcaagc gatgcaatat cgctttcatt caaggcaatc agtgggttta
     2761 cgtcatggag tgggctcaac accagtcaga gccgcatgta cacgcaataa tcatggtgaa
     2821 cgaaagaagc attaatgggc aagctggcaa aattgtcagc aaatgggtaa cactggcaaa
     2881 caatgcaatc ccatcaaatg ttcctcccca gtgggatcta gatttgaaag gtcagcaata
     2941 ttcagagcct acaaagtgta taggtaaaca gattgattac atggcaaagc ccgaaactaa
     3001 gttaaatgac tttaatagag aggcgagcaa gagcctatat gattggtcag atgccaacgt
     3061 ttggggttat tcgaatggct gggcgcggca tgagataaaa aaagaaacac tttctatcac
     3121 tggctttcat gctgtaagac gcatactaaa agccgttcag gcatcaagga aagacaacta
     3181 caaagctaag cgacagatac agcgtacatt gaaacgacaa gacataaatc gatcaccaat
     3241 caccccgttc caagcaccgt taattagcaa acacgattgg gatttatcag tgatagcggt
     3301 caccaaagag gcagagacag agcgcaggga attactatcc tcacagataa taaactcgaa
     3361 caaagtacaa acccacccac tgttaaggat gttaagaaga gcacgagtga gagcatccag
     3421 agccaaaaga gaaagcggag gttatgacca ttactactag tagtgacaga tcgtgacaag
     3481 gcggtgcaaa aaacgaccaa taataagaga gatatgcctg ccaaaccgcc aaaagcgcgg
     3541 agcgcggcaa acgccgcgag aggcgcaaga aggcgaacct atccattgaa acggtcagaa
     3601 gcgcatcgcg ctcgacgacc gaagtcccca ctaacaccgt taacgaaaga tcaaagtctt
     3661 agaaaaaaat gacgtttctt tgatggactt ccatccgtaa aaatcaaatt ttgagaaaaa
     3721 gtacttatag aaattaatcg ctctataagg ataagttgac gttagtttaa agtgataaaa
     3781 cattaaagaa aaaacgtaaa atcaaataca taaaataagt caccaaggga tcattttcag
     3841 aaaacaacgt caacgtaacg tcaactctgt aagaactttt ttggtcactt tttgggttga
     3901 atgtacgtaa aaatccccag cagtgaaatt tgagcgcgta agcacaattc tgaggggctt
     3961 tttgtctgta ggttaggcag tgtaactaac aatacaagaa gttagctctg actaactatt
     4021 taattaactt ttcaatctaa ttgcgttaga tattataccc taacgcaata tttttaattc
     4081 aataatgtct gtaaattgtt attacttata attatacatt taactatatc tacattggct
     4141 gcttccaatt actacttacg taacggaatc tcggatcctc tgtttttggc tcggtataat
     4201 aagaatcatc aatagtaaag ttcgacgcta atttatcccg aagcccacct aaacgttcaa
     4261 tgcttgttag ttcgttagta acatccttga aaacggtaga aaatgcttta tgttgtttag
     4321 acttcataaa ttcaggagtg ctcagtgcaa actctttatc aggcaaacct ggaataccca
     4381 cgagtcctgt caatgaattt atacatttta tcaaaaaact gccttgaata ccttgatttg
     4441 atggaacacc gtagcttcgt ttaatatctc cagatatttt atctaaatca ctt
//
LOCUS       DAZFBR010000213         4450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFBR010000213 DAZFBR010000000
VERSION     DAZFBR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.213"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..384
                     /locus_tag="ACHJZ2_004815"
     CDS             <1..384
                     /locus_tag="ACHJZ2_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4570764.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRSKGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAP
                     KRPPGPTPIELLKQEYERRKAAGFV"
     gene            419..1117
                     /locus_tag="ACHJZ2_004816"
     CDS             419..1117
                     /locus_tag="ACHJZ2_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4570765.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            1139..1363
                     /locus_tag="ACHJZ2_004817"
     CDS             1139..1363
                     /locus_tag="ACHJZ2_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570766.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1360..1716
                     /locus_tag="ACHJZ2_004818"
     CDS             1360..1716
                     /locus_tag="ACHJZ2_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HFO4570767.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1749..1901
                     /locus_tag="ACHJZ2_004819"
     CDS             1749..1901
                     /locus_tag="ACHJZ2_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570768.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1898..2209
                     /locus_tag="ACHJZ2_004820"
     CDS             1898..2209
                     /locus_tag="ACHJZ2_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570769.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2336..2899
                     /locus_tag="ACHJZ2_004821"
     CDS             2336..2899
                     /locus_tag="ACHJZ2_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570770.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGN
                     FRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGSCLD
                     EAEQELIVTINEYASQIAVEATQGENQ"
     gene            3009..3113
                     /locus_tag="ACHJZ2_004822"
     CDS             3009..3113
                     /locus_tag="ACHJZ2_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570771.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            3357..3512
                     /gene="hokD"
                     /locus_tag="ACHJZ2_004823"
     CDS             3357..3512
                     /gene="hokD"
                     /locus_tag="ACHJZ2_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4570772.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3680..3958
                     /locus_tag="ACHJZ2_004824"
     CDS             3680..3958
                     /locus_tag="ACHJZ2_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570773.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3960..>4450
                     /locus_tag="ACHJZ2_004825"
     CDS             3960..>4450
                     /locus_tag="ACHJZ2_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570774.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLA"
BASE COUNT         1204 a          989 c         1244 g         1013 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgttcaaag
      181 gggattgagc gccagatgac cgaaggggag ttaaaacggc ttgtggaaag gcagctgacg
      241 aaatgggcaa agcatgttgg taatggcctc agtgttccgc cagtccggcg acaactggca
      301 gcacccaaac gcccgccagg gccaacgcca attgaattgc tgaaacagga atatgagcgc
      361 cggaaggcgg ccggttttgt ctgagttgag aagtaatttt taccgggagg aaaatttaat
      421 ggaaaccgtt tttgacgcac tgaaagcact gaaaaaagcc tcgtcgcacg agattgcagc
      481 ccgtcttgaa atcagccgtg acgatgctgt taccgaactc tggaagctga agcgtcgtgg
      541 cgaagctgat aacaaggggt cgatgtggtg gctgacgagt gaggcaactg aagcggcccc
      601 aaaaaccact gcagagatgc tgattaacgc gattgaacag catggtcctc agtcggctga
      661 cgaactggcg ttaatgttcg ggattacctc ccgccgggcg aattcatcac tggcgatggc
      721 tgtcagcaaa ggtcgtctga ttcgcgtaaa tcaggacggt aaatatcgtt actgcatacc
      781 gggcgataat ttaccggcag agccgaaagc tgcatcggta acggaaaccg atggtaaagc
      841 ctttcctcag ccggcaggtg ttgcgttacc agtccgggaa gcggaaacac aggaagaaat
      901 taaaactgaa agtgtggcgg tcacagtgca gtcacagtcg tcgttcatca gaaagcatcc
      961 ggatggtctg attttaccat cgctgcatgt ggctaaccgc gagctgcgcc gggcaaaagg
     1021 tcaggttcag aagtgggagc gcttatgcgc cgcgctgcgg gaaattaata agcatcgtga
     1081 tgtcatacag aaaataacga caggagataa cgggtaattt ttacagcagg gtgatgatat
     1141 gaaaatcaga tatcatgatt tcggccccgt gtcacatatg cttatttcaa gtacggtgct
     1201 ggaaacaaga aaacataatc acatactgga tatgctgcgt cttgctgacc cgtatctggt
     1261 aataaacacc agtggaattt tctttctgag aagcactgtg tcaggaaaaa catcgcatgt
     1321 gcttcgggca tataaaacag cagtgcggga ggaaggagaa tgagcgagat taactatcag
     1381 gagctgcgtg ttgagctggg agcagcaaaa aaacgcatag aagagctgga ggctaatcgt
     1441 gtggtgcttg aggtggaaaa tgagaggttg aaacacgcaa tggccgtagc ccttgagcat
     1501 atatccgtca cagatgcagg acaggcaggt gtcgctgcaa tgattatcta tgatgccatg
     1561 taccacagtg aaaaaactga cccctacgct tttctggcag aattgcgggc gcagggtgtg
     1621 gagatggtgc gcgaacatcc agcaatcaaa ctttgctctt tgacgcacat atgtgacgag
     1681 ttagtcgcag agcttcgcaa aggaggcaac aagtgagtaa aacgaatccg ggctgggcaa
     1741 ggccacttat ggcgaaaaaa catcattatt ttgctgaagg tgaaataaca agtatttgcg
     1801 gtgggtggat gtattttggc aatgagcgtg agccggatac attcgaaagt ccagatgatt
     1861 gtaaaaagtg tcgtagaaaa ttaaataagg ggggtaaatg agcgtaatta aagaaatgcc
     1921 agtggaacgt aatgaatatg gctgctggac gcatcctgaa tatgaaaaat tctgtgatgg
     1981 tcgggaatat atttcaacgg aagagtttaa cgcctggatg gagaaaaata atcttcagtg
     2041 ggctatccgt agtatggatg aagatgattt taatctggac gcagatggcc ctgatatttc
     2101 agcatgggaa cctgagcgac cagaaggtga gggatggttt atcggttcga tacatgacac
     2161 cgaagatggt cctgtttgtg tatggctgag aaataaggcc gaagcataaa ggctataaac
     2221 caacaactaa acactgaaaa tttaaatcag aaatgatttt tattaaatcc ttaaccggag
     2281 ggattcctgc accctcagaa catcaggaga ccgcccgaaa gggcggtagt gaaaaatgac
     2341 tgaattaacc aaagaatggc tacagaacac gattaccgga attgagtcat cacgggatga
     2401 aataccgttc ggactcgatg aagatcaaaa caacatgctt accgcattaa aaattgcact
     2461 ggcatcactg gcatcagtat cggatgaacg ggcagcctat gaattattta tggagaagcg
     2521 tttcggggaa tctgtagatc gccgcagagc aaaaaatggc gatagagaat acatggcatg
     2581 ggatatggcg cttggctgga ttatctggtg tcaccgcgcc gccatgcttc aggctggaaa
     2641 ctttcgggaa aataagggtt cgtcaaccaa taattttcgg ataatctcgg aaacgtcaac
     2701 caactctccg gcaatccctg atgaggtgtt gtccgcaatc ctgaaggtcg ccaggcttcg
     2761 tgcagatttc gatgcttctg aagtggacag gcgaggtatc ggtagttgtc tggatgaggc
     2821 cgagcaagaa cttatcgtta ccattaacga atacgccagt cagatcgcag tagaagcgac
     2881 acagggggag aaccaatgag ccggccagat gcatttgcag gcgttgctat agctattgct
     2941 tttctagtat atgttatttg tcggtggggg taaaaacgtt cgccgggatt cacaccaagg
     3001 gagggaatat gtcggatgat atttcactgg taatggaagg cgctctggct gttattgctg
     3061 ttgtgggtgt ttactgcctg gttgtgtttt tgatggagcg cctggggaac tgaattcatt
     3121 ccgtatggga attcccatat cgggcaaaac ggtttgctgt aaagcgagag ttaagtagaa
     3181 ttgctgcggg tgcttgaggc tgtctgcctc gggcatgcca ccgtaaggca gacagagaaa
     3241 agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta
     3301 tgcttcacaa acgtaggtta gcctcttacg cgccgaaagg caaggagaag caggttatga
     3361 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
     3421 cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg
     3481 ctgtcttcac agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc
     3541 caccgctgat gtgtcaggca tcctcaacgc acccgcactt aacccgcttc ggcgggtttt
     3601 gttttttctg gtcgttctgg tttacaatcc atccgtcagc ctgaacaact ggcacctgct
     3661 gcgccagcag agaaaacaga tggcgcacga taccaaattt tacaattcgg ataactctgc
     3721 cgcccctgcc agcaggcacg ggcggcgttc tcatgcattc aaatctgact ggtatcagca
     3781 cgacccctgc accgaagaac aggctgaatg gctgattcag tgttaccgca ggcgcggata
     3841 cgaggttaag aaagccctca gtctcgatta tcgtcactgg ataatctccg tcaggcttcc
     3901 ttactccgaa cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg
     3961 tgcgggtatt acttcgacct gttctggtac cggaactcgg gctggtgatc gttaagccgg
     4021 gccgtgaatc catgccggta ttccacaata cccgggtact ggtggagccg gaaccgaaaa
     4081 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgttcg ccagccgctg gcggaggata
     4141 aatcattact gccatttttc agcgacgaac gagtgattcg tgctgctggt ggcgctggcg
     4201 cactgtctga ctggttactg cgccatgtta aatcctgcca gtggccacac ggcgattatc
     4261 accacagtga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     4321 gcgacaacca gttgcgtgac cagacctccg aatcacttga gcaacttgct caccaaaacc
     4381 tgtcagcatg gatgattgac gtcattcgtc acgcaatgaa tggcacacag gagcgtgaat
     4441 tatcgctggc
//
LOCUS       DAZFBR010000214         4349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFBR010000214 DAZFBR010000000
VERSION     DAZFBR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.214"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            332..738
                     /gene="ipbA"
                     /locus_tag="ACHJZ2_004826"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             332..738
                     /gene="ipbA"
                     /locus_tag="ACHJZ2_004826"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
     gene            1164..1958
                     /gene="hyxR"
                     /locus_tag="ACHJZ2_004827"
                     /pseudo
     CDS             1164..1958
                     /gene="hyxR"
                     /locus_tag="ACHJZ2_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
     gene            2112..2873
                     /gene="hyxA"
                     /locus_tag="ACHJZ2_004828"
     CDS             2112..2873
                     /gene="hyxA"
                     /locus_tag="ACHJZ2_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO4570775.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIGTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANSSGGLSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            2915..3997
                     /locus_tag="ACHJZ2_004829"
                     /pseudo
     CDS             2915..3997
                     /locus_tag="ACHJZ2_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            3987..4175
                     /locus_tag="ACHJZ2_004830"
     CDS             3987..4175
                     /locus_tag="ACHJZ2_004830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4570776.1"
                     /translation="MLWLRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTK
                     GDDIENPLQGVVGINVTW"
BASE COUNT         1258 a          904 c         1001 g         1186 t
ORIGIN      
        1 tcactaaaac attagacgta acacacatcc cccacgccct gtaattcata gcttttatat
       61 ttattttttc acatcaatgt agataaaaac acgcattaaa aatggcttgt agaaaagcag
      121 cacaataaat agttcatatt tagtctattg cgtagaacta ctattcaaaa aatcttatta
      181 atgagataat gagattacat cgataacgtt ctgattgcag gcatacttat ctgggtgtgc
      241 ctctcttttt tacaactgta cacggtggcg tatgacgaga aaatatctca cacaggatga
      301 agtctacagg ctgatggatg cagcccagag catgtccttt cctgaaagaa atcgctgtct
      361 aattatgatg gctttcattc acggttttag ggccagtgaa cttcttgatt tacgtttatc
      421 cgatattgat gcatcaggaa aacaacttaa tattcggcgg attaaaaatg gtttctcgac
      481 aacacatcct cttcttcctg atgaatataa tttgatcaag ttgtggctta agcagaggaa
      541 gttaattgaa aacggagttg aaggagactg gctttttctc tcccggaaac gccgccctat
      601 cagcagacaa catttttttc tatcattcgt gaggctggaa gacgtgcagg attagctgta
      661 aaagcacatc ctcatatgtt acgtcatgcc tgtggttttg ctctggctga caatggtgtc
      721 gatacccgac tacttcagga ttatctgggt catcgaaata tccagcatac tgtcagatac
      781 acagccagta atgctgctcg atttaaaggg gtgtggaaaa aaaagcctcg ttaaaacagt
      841 accttctgat tcaaaatgtc agattcctac gttttcctca attcttccat aaaaaaacag
      901 cccattcaat tcaataaaac acataacatg ttgatatttc tcgccaaagt aaaataaccc
      961 catgatttga aatgattaac cgccaaggtc ataccagtga ttaactttca ctcatgttgt
     1021 taaatgcaac caaattaaaa acaataaaaa tcaaacaaat tagatttata ttccaagaaa
     1081 attaaatatt caggcaatta aacaaaacct gacattttga atcaaaatgt caggttttgt
     1141 agctatggcg tggacgagtc tgaatgaaga agcctctggt tattatcagt gcctgtcagt
     1201 ttaccaggct ggcactggag tctctcatcc cagcggacag gtatatcgtg cgggtgtaca
     1261 gcaacgtcac tacagaggtt gaattcgttt taaagacctc ctgtggctat ctgttggctg
     1321 atatccctta gcttccttcg ggggagttag ttcttctggc ttgtcttgtc cgcttgcgga
     1381 gtgatgccgg gcaatggcag gtctgcctga ccggtgactc cgactggttc gataatgctc
     1441 ctgctgcaag tctgtataac ggcttcccac gcatcggcac catgctgaca gcgagtcgtt
     1501 atcagaatca aattatccgc tggttactac gtcctttgcc cggcgatatg agcgatttct
     1561 ggctcctgac tgcacgggag ttgagcgtgc ttaagattct gatgcagggc atgagtttca
     1621 gtgagattgc ccgttatgaa caacgcagtt caaagaccct gcatgcgatt gccacacggg
     1681 cattaatgaa acttggactg ggaactctga gcgatttcag gctgctctat acgggatgcg
     1741 gtgacagcag agtgaaacgg aatatacggc tacatgcaag atatgtcgga caacattccg
     1801 ctcatcgact cactcagtat gtgcagaaca tggtatccgg aattctttat ccagagaaag
     1861 ttgccgttgg tgaatcaata aattctgacg ccatttccgg aaaagtactt cccggacaaa
     1921 gaaaaaatat ttctaaaaaa agaccaggat tgttatgaca caacagcgtt gttactaccc
     1981 cccactgcgg cgcgtacgga ctcgctacag tgtaggggcc aaaatatttc tactgctctg
     2041 ggctgcagga ttgggaggca cgctgtacag cctgtacagc gtgctggcgt actgacatct
     2101 gtgagggaag gatgcgtttc gttcttttct gtccctcagg aattgtacca gcgcagttcg
     2161 cagctctatc aaccggttct gtcggcaatg ttacctgcat cggaacagaa gaggagttac
     2221 gcaataaatt gcggcacagg ccacagagtg tcgtgatatc ggcagggcga cctgccgaat
     2281 gcgctgaaat gtggttccga ttttatcggg atcactcatt cgtggtggtg ttgtgcgtcg
     2341 cgccattttt tctgccaccg gatgtgagta tttctggcgt tctcaaaaac ctgaggttac
     2401 tgaaaccagg aatgtcagta gagcatgtca tcagtattgc aaattcctct ggtgggttat
     2461 cagggctgaa acatgcagaa aatttgccag ttatggacag ttactctgtc tttatgaaag
     2521 aggtgaataa ccgcacaaag acaatcgtca tgtcagaacg cttcccggaa aagcaaaaga
     2581 aggtgttgtc gttgcttctt gccggacaca gctgggaata cagtgcgcag tttctcaaaa
     2641 cggggataag acagatatgg cttgccgagc agtcgctaaa aaagcgttgg ggaataccgg
     2701 acagcatgtc attgcgggaa gccctgctgc tgagcagcaa taaatttcac ggtgatggaa
     2761 atgcattgga aaaaacgaat gccatgacat tgcgtgaaaa cggaaatacc aacaggtatt
     2821 ccgtggtggt aaatgcaggg acgcaggttt tatctctgca taaatataaa taagtgggaa
     2881 attgaaatta gttaccagaa tggagaattt cttcatgaaa aacagtaagg cattttaccg
     2941 cagcgcatta gcgacagcta ttgttatggc tctttctgca ccagcattcg ctgctgataa
     3001 cgcggtatca actgattcgg ttacgctgaa taaagataag acgactctgg atcaagatgt
     3061 tgttattagc aatacagcag acaaacagat tacagccgta acaattaatg cggcagatga
     3121 agaccttaat gttgcttttg ctggtcatga tattactgct gaatcaacgg cagacaaaaa
     3181 attcgttgaa ggtgtaaaag ttagtggtaa caaaaatgtt gtgattaatg ctacagactc
     3241 caccatcaca gctcaaggtg aaggcaccta tgtccggact gcaatggtca ttgattcaac
     3301 tggcgatgtt gttgttaatg gcggtaattt cgttgcaaaa aatgaaaaag gtagtgcgac
     3361 agggatatct ctggaagggg ccaagggaaa taatgtcacg cttaatggca caaccataaa
     3421 tgctcaaggt aataagagtt ccagcaacgc ctccacggca atttttgctc aaaagggtag
     3481 cttattgaat agttttgaag gtgatgcaac cgacaacatt acccttgctg gctcaaatat
     3541 tattaatggt cggattgaaa caatagttac tgccgggaat aagacgggaa ttcatacagt
     3601 caacctgaat attaaggatg gctcagtaat tggggcggct aataataaac aaacaattta
     3661 tgcctctgct tcggcacaag gcacaggttc agcaacgcaa aatttaaatt tgtctgttgc
     3721 tgattcaacc atctactctg atgtcctggc cctttctgaa agcgagaatt cagccgctac
     3781 cacaacaaat gtaaacatga acgttgcccg ctcttactgg gaaggtaatg cttatacttt
     3841 caatagcggc gataaagcgg gtagtaatct ggatataaat ctttccgata gttcagtctg
     3901 gaaaggcaaa gtttcagggg caggaaatgc cagtgtatct ctgcaaaacg agtctgtctg
     3961 gaatgttacg ggatcctcaa ctgttgatgc tctggctccg tctgggtgat gagtctgtca
     4021 atgccagcat tgacggtgca gcattccgcg tgggtgcagg tgtacaggct gatatcacca
     4081 aaaacatggg agcatatgca agccttgact acaccaaagg tgacgacatt gagaatccgc
     4141 tacagggtgt agttggtatc aatgtgacct ggtaagaata aagcagtcgg ttcccctgtc
     4201 gactgttata ccccctgcag gcagcagtat gctgcctgca ggctataaat atccagtatt
     4261 catgatgctg gctttttgct gatatttaac ccgaacattc ctgatataga ctattaattc
     4321 atttatctgc ggcagatttt attttctgg
//
LOCUS       DAZFBR010000215         4234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFBR010000215 DAZFBR010000000
VERSION     DAZFBR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.217"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..148)
                     /locus_tag="ACHJZ2_004831"
     CDS             complement(<1..148)
                     /locus_tag="ACHJZ2_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570777.1"
                     /translation="MYNFITIMYDVFSCFGVLAKNQNSRDIRNIKNFSSHQHSLGDMF
                     DELIN"
     gene            345..1081
                     /locus_tag="ACHJZ2_004832"
                     /pseudo
     CDS             345..1081
                     /locus_tag="ACHJZ2_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1260..1556
                     /locus_tag="ACHJZ2_004833"
     CDS             1260..1556
                     /locus_tag="ACHJZ2_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4570778.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1864..1980
                     /locus_tag="ACHJZ2_004834"
     CDS             1864..1980
                     /locus_tag="ACHJZ2_004834"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570779.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            2199..3584
                     /locus_tag="ACHJZ2_004835"
     CDS             2199..3584
                     /locus_tag="ACHJZ2_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO4570780.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIMIN
                     ELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGPLRHCREYAAKNAEYMA
                     RLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPLTFDNSALTVPVRLIPE
                     ELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWLLDLLYEQNRVVPVIEG
                     KTLCVAPNFDL"
BASE COUNT         1274 a          790 c          923 g         1247 t
ORIGIN      
        1 tgtttattaa ttcatcaaac atgtcgccca gtgaatgttg atgtgaggaa aaatttttaa
       61 tatttcggat gtcacggcta ttctggtttt tagccagaac accaaaacat gaaaagacat
      121 catacattat agtgataaaa ttatacataa cattctcctt ttattgcact atataaaatt
      181 ttaacattat atcattttgc ttttctgtta actggttttc atttttgaat caatattttc
      241 tgaacagggt aatagtacac gtattaacag attatgtcag aaggccccgg tgatgccttc
      301 tgacgataga ttaatgttgt attctattgg tatctggaga ccatatggca acagttacag
      361 tatattctcc ccgctgtaat tcctataaag tgtaccgtta tgatcacagc acctcagatc
      421 atgagcgttt ccagtgctaa tcctgcaaac gtgtatttca actgaattac atctacgaaa
      481 cccgaaaacc aggcattaaa gagcaaattg ttgaaatggt tcataacgga gccggggttc
      541 gcgatagctc cagaacgctg aaggtggata tcaacaccgt cattctcact ttaaaaaacg
      601 ctcaccacgt aaagtaacca tatctccagc cgccagtgtc gatgtggcac ttatctgtga
      661 acttgatgaa caatggagtt ttgtcgaaaa caaagctcgt cagcagtggc actggtacgc
      721 gtataagacc aaagctgacg gtgtgctggc ttacactttt ggtcctcgca ctgatgaaac
      781 atgccgtgag ttgccggaat tcctgaagcc attctccgca ggcatgataa ccagagacaa
      841 ccggagcagt tataccagag aaatgccaca ggataagcat cttgtcggca agatatttac
      901 ccggcgcatt gagcgcaaca atctgacact tcgtactcac atcaaacgac ctgcccggaa
      961 aacgatttgc tttttacgct ccctggaaat tcacgaaaag ccattggttc atttatcgaa
     1021 aacacatgta ctactgacag gagtcattac cagggcctct tttgctgttt ttttgccatg
     1081 aatgataatg agaatcgaca gcgataaggg aaaagtaaaa taacactaat gctgtcgttt
     1141 tctatcatat tgatttttaa ttatttattg tcttttttct caacccattt ttttacgcag
     1201 acaaatttac ccggggaata caaactgtaa cgacagttca ccacactatg ggggataaaa
     1261 tgccagatca atccggacac tgcaggactc cttcacgcat cttacggatg cctgaactga
     1321 gccaattgtt aggtatatct cgttcgacaa tttacgaaaa aatgaatccg ttatcgaaat
     1381 actacgatgc aaccttcccc aggccggtac gtcttggtag tggttctgtg ggctggcgtt
     1441 catcagctat tgacgaatgg cttactttac atactgtacc tgccagatca gcagtcaaag
     1501 gtgttaatga cgaagtgaga catacgcttt cttcagatat gaccatgagc agataagttt
     1561 ttgtcatcac gtgatcacct caggaggaaa aagaagcata agaacaattt agccagaagt
     1621 ttaattgata aacagaattt ccagcattca ttgttgaaag agcagctatt gtcgctattg
     1681 tagcggtaat tgctgctata accgaaacgt attacgaaaa aggaagcatt tatgccttac
     1741 atcaaaccgg atatgtcgta ttcagtatat gcatgaccat caatgatagg gaaggcaata
     1801 attaaagctc aatacaataa acaagtacct ctgactctgg ttgcaatgtc agtattaaca
     1861 gcgatgtcaa ttgcctgtca gaatcaggtt gacgtatgca gtcccggaaa tttacgtggg
     1921 gccgttaata tttacactat ggttctggag aaagggaaaa gaccggggga aaggtattag
     1981 tgaaataatt tccggaggta ttaagaatga gaaatatgaa tgatatttcg tggtgaataa
     2041 tacagtcttc actgaattaa ccttatcggt ttattaaaca tcagatctac aaatgctctg
     2101 tatttctcag agcatcaggt ctgaatagca gctattttcg ctattgtagc gggatttgct
     2161 gctattttac taacccatta atgaaaagag aggcttttat gtgcttactt gcaccagaaa
     2221 atccttatcc gatatatgca ctaccaccgc tggtgagaaa tgcaataatt gaaactcaaa
     2281 aaaatacaca ggcacctttg gctatggtgg caacatccgc attaacagcg atctcaattg
     2341 cctgtcagaa tcagattgac gtgtgcagac ctggaaattt acatgggcct gttaatcttt
     2401 actctctgat tctggctgat tccggtgaaa ggaaaacgac cgtggataaa gtgtttatga
     2461 aagcatttta tctcagggat gaagccctgg cggatgaata cgcgaaactg gttgagaatt
     2521 acagtacaga aaaggaaata tgggagcaaa aacaaaaagc gctggaatca aaatttcata
     2581 aagagattcg tgccggtaaa gattacaagg caacagaatc agagcttgaa acgcatctga
     2641 ataaacctcc tgttccgccg cagatacgtc gaacaatttt taatgagaca acgatagagg
     2701 gaatgttaaa atattactcc gatagcaatc gctcttttgc tcttgtatcc agtgaagggg
     2761 gggtaatttt tgacagccgg gccatgagta aactgggaat tattaacact ttgtgggatg
     2821 gaggttctct tttcatcgac aggaaatcat ctcccggaat taatttgaag gagccaagac
     2881 tgacgatatc ggtgatgatt cagcctgatg tttaccacaa aggtttttgt acgcgaaaaa
     2941 aagaaattgt gaaaacgtca ggacatcatg caaggttttt gatgtgtcaa ccaacatcaa
     3001 cgcaggggac aaggataatg atcaatgagc ttattgatga aagtctggcg atgagtggtg
     3061 aacgacgttg ccttcacttt tctcctcagg ctgccagaat ctggacggat tattacaatg
     3121 atgtggaatc gaagctgggg ggattggggc ctttaagaca ttgccgggaa tatgctgcta
     3181 aaaatgcaga gtatatggca agactggcag gacttattta ccattcgagc ggtgaagagg
     3241 gggagatttc cccttacact gcagaaatgg cgagagaatt agcaatatgg tacggtaatg
     3301 agtatgtgcg gttgtctaat ccgttaactt ttgacaactc tgccctgacc gtacctgtgc
     3361 gactcattcc agaggagctt gaacttttca actggataaa aagctattgt attgagaagg
     3421 ggatcctctg tatgaaaaaa aatgacattt tacagcgtgg tccgaaccgt ttccggaaaa
     3481 aggataaaat caactggtta ctggatttac tgtatgaaca aaacagagtt gtaccggtta
     3541 ttgagggaaa aacgttgtgt gtcgcaccta actttgacct ctgaacgact taatcgttga
     3601 gtacatatag tctcctctgc tacttgcgga ggagactaat gtctttacca tgtcagcgtt
     3661 gtattagaaa actctggtac aggtcagtca gtaaatataa ctggtgtaac cactatttac
     3721 tattggatag agtgttgctc aggatattgt cggctttagt ctgaggtgag gtggggcctt
     3781 cattgttttc gtgttcaata aataatttag gttaattagt attattagtg gaagttatgt
     3841 gatctgttat gttgtcttgt acgcacttaa cactggttac gtaccggaac actattagag
     3901 tatgttatgg ctctgttaca gtttataggt atgaaaaaat ataatctttc ctgtatgaaa
     3961 taatattctt atggtgtgtg gggtatggtc tgtataccat catttttatg gtgaagttaa
     4021 atatctgtag aggccttaag ctgggtcgct gtttttactt aatggtacag aagtattaat
     4081 agcggtgttg cctgtggcta cccgatactg gcttttgtga atgtcagatt acttcctgta
     4141 agtgattgaa tggtacttcc ttaaactcaa ttctatgaga tgtgttttta ccattgtaat
     4201 tcgtactggt cctcagttta agtgagtctt actg
//
LOCUS       DAZFBR010000216         4229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFBR010000216 DAZFBR010000000
VERSION     DAZFBR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.218"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..209)
                     /locus_tag="ACHJZ2_004836"
     CDS             complement(<1..209)
                     /locus_tag="ACHJZ2_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4570781.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHJZ2_004837"
     tRNA            complement(324..399)
                     /locus_tag="ACHJZ2_004837"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHJZ2_004838"
     tRNA            complement(406..480)
                     /locus_tag="ACHJZ2_004838"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHJZ2_004839"
     tRNA            complement(597..681)
                     /locus_tag="ACHJZ2_004839"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHJZ2_004840"
     tRNA            complement(690..765)
                     /locus_tag="ACHJZ2_004840"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHJZ2_004841"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHJZ2_004841"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO4570782.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHJZ2_004842"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHJZ2_004842"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO4570783.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHJZ2_004843"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHJZ2_004843"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO4570784.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1084 a         1049 c          946 g         1150 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcactat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagaca tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagctatta agactgccat gacgttgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccttgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcacctg agtgaaattc gccattcgct aacagcgcga ttaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc aaccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgccct tctgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccca
     4201 gtcttccgac cgggccttct gcttaattt
//
LOCUS       DAZFBR010000217         4192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFBR010000217 DAZFBR010000000
VERSION     DAZFBR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.219"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(37..2013)
                     /gene="speA"
                     /locus_tag="ACHJZ2_004844"
     CDS             complement(37..2013)
                     /gene="speA"
                     /locus_tag="ACHJZ2_004844"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO4570785.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2022..2153)
                     /gene="yqgB"
                     /locus_tag="ACHJZ2_004845"
     CDS             complement(2022..2153)
                     /gene="yqgB"
                     /locus_tag="ACHJZ2_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO4570786.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(2247..2390)
                     /gene="yqgG"
                     /locus_tag="ACHJZ2_004846"
     CDS             complement(2247..2390)
                     /gene="yqgG"
                     /locus_tag="ACHJZ2_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO4570787.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2501..2752)
                     /gene="yqgD"
                     /locus_tag="ACHJZ2_004847"
     CDS             complement(2501..2752)
                     /gene="yqgD"
                     /locus_tag="ACHJZ2_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO4570788.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2808..3962
                     /gene="metK"
                     /locus_tag="ACHJZ2_004848"
     CDS             2808..3962
                     /gene="metK"
                     /locus_tag="ACHJZ2_004848"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO4570789.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1070 a         1140 c         1012 g          970 t
ORIGIN      
        1 cgtaaataaa tcgacccgcc agcagaataa tcttaattat tcatcttcaa gataggtata
       61 accgtacaaa cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc
      121 ggttttcttc acttgatcac ggaactgagt taacagcgtt ttcggatcga gctgtacata
      181 ttgcagcatg tccgccacgg tatcgccttc gtcagacagt tccacttcta cactaccgtc
      241 agggaagacg aacacgtcaa ccgcttcggt atcaccgaac aggttgtgca tgttgccgag
      301 gatctcctga tatgcgccga ccataaagaa accgagcatc ggcggattct ctggatcgta
      361 ctccggcata ggcattgtcg tggcaatacc atcaccatcg atatagtggt cgatagcacc
      421 gtcagagtca caggtaatat ccagcagcac agcgcggcgt tccggcactt gatccagccc
      481 ttccagcggc agaaccggga acaactggtc gatcccccac gcatccggca tcgactggaa
      541 cagcgagaag ttgacgtaca ttttgtccgc catacgttcc tgcagctcgt cgataatcgg
      601 acgatgagca cggttttgcg gatccagctg cttttgcact tcatggcaca tgctcaaata
      661 aagctgctca gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg
      721 aatgtcgtgc agatccatct gactgtcgtg taaccattca cgcagagaac ggcgagttcc
      781 cggttcgtgc atctcctgcc aggtttccca catgctttgc agcgcgcgcg gcgcatcttc
      841 tgcaggcgcg gtcggcaccg tgtattcgtt acgttccacg ccgatgatat tagacaccag
      901 cacggtgtga tgcgcagtca ctgcacgacc cgattcggtg attaccgtcg gatgcggcag
      961 accgttttct tcacacgcat caccaatcgc ccagatgatg ttgttggcat attcattgag
     1021 gccgtagttc accgaacagt cggactgcga acgagtacct tcataatcca cgcccagacc
     1081 gccgccgacg tcgaagcact gaatattgac gcccagcttg tgcagctcaa catagaaacg
     1141 cgcggattca cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg
     1201 gaagtgcagt agttgcaggc tgtcgagacg cccggcttca cgtagggttt caaccagttg
     1261 cagtacctga gtcgccgcca ggccgaactt cgatttttcc ccgccggagg actgccattt
     1321 accggagccc tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg
     1381 ttctgcttca tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt
     1441 gtgccccatc ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccatt
     1501 gcagacgatg acgctacggg tcatgccagc atgtgccagc actgccatca actcggcttt
     1561 ggaaccggct tccagaccca gcggttcgcc cgaatgaatc agggactcaa tcacgcgacg
     1621 gtgctggtta actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc
     1681 ccgcgcacgt ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa
     1741 acagaacagt gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag
     1801 atcgacgcga gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt
     1861 aacgtcatag tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc
     1921 ttcctgggag ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga
     1981 cgaaggcaaa cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt
     2041 gtaaaacagt taacgactat cgcagcctgg aactgcgata acaacccata agcacatgga
     2101 ttttccagca gtgaatgctg acgctccaac tgcgcaaccg gtttcttttt cataacatta
     2161 ttaagcacat aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt
     2221 tttacaagag cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa
     2281 gcgccgagat gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac
     2341 gaatcttcac cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat
     2401 cacctccacg gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc
     2461 tggaagtggc aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt
     2521 tataccccac aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag
     2581 gaaaaatttc cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc
     2641 gggtgaagaa ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac
     2701 tcagactgcc agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc
     2761 cacacaacag tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc
     2821 tttttacgtc cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg
     2881 atgccgtttt agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct
     2941 acgtaaaaac cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca
     3001 tcgaagagat cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct
     3061 ttgacgctaa ctcctgtgca gttctgagcg ctatcggcaa acagtctcct gacatcaacc
     3121 agggcgttga ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg
     3181 gctacgcaac taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc
     3241 tggtacagcg tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg
     3301 cgaaaagcca ggtgactttc cagtatgacg acggcaaaat cgttggtatc gatgctgtcg
     3361 tgctttccac tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg
     3421 aagagatcat caagccgatt ctgcccgctg aatggctgac ttctgccacc aaattcttca
     3481 tcaacccgac cggtcgtttt gttatcggtg gcccgatggg tgactgcggt ctgactggtc
     3541 gtaaaattat cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg
     3601 gtaaagatcc atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa
     3661 acatcgttgc tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg
     3721 tggctgaacc gacttccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac
     3781 aactgactct gctggtacgt gagttcttcg acctgcgccc atacggtctg attcagatgc
     3841 tggatctgct gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac
     3901 atttcccgtg ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt
     3961 aatctttctt cacctgcttt caaaggccag cctcgcgctg gcctttttct tttacctcac
     4021 cgcggtggat aggcgttcac gccgcatccg gcaaaaaaac cgcctgcaca ataacatcat
     4081 tcttcctgat cacgtttcac cgcaaattat catcacaact gaaaccgatt acaccaacca
     4141 caacagacaa agatttgtaa tatttccata ttattattcg gttttcacag tt
//
LOCUS       DAZFBR010000218         4187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFBR010000218 DAZFBR010000000
VERSION     DAZFBR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.220"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..835
                     /gene="yibB"
                     /locus_tag="ACHJZ2_004849"
     CDS             <1..835
                     /gene="yibB"
                     /locus_tag="ACHJZ2_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO4570790.1"
                     /translation="TAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALENEMII
                     FTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKNP
                     EYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDE
                     SKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKITL
                     NNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSR
                     K"
     gene            1110..2306
                     /gene="kbl"
                     /locus_tag="ACHJZ2_004850"
     CDS             1110..2306
                     /gene="kbl"
                     /locus_tag="ACHJZ2_004850"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO4570791.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2316..3341
                     /gene="tdh"
                     /locus_tag="ACHJZ2_004851"
     CDS             2316..3341
                     /gene="tdh"
                     /locus_tag="ACHJZ2_004851"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO4570792.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            3580..>4187
                     /gene="waaH"
                     /locus_tag="ACHJZ2_004852"
     CDS             3580..>4187
                     /gene="waaH"
                     /locus_tag="ACHJZ2_004852"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO4570793.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNAL"
BASE COUNT         1060 a          902 c         1084 g         1141 t
ORIGIN      
        1 cactgcatat tttgacattg gtagagggga ttggactgct aataaagggt tccgtgaaaa
       61 actagctcgt tcagttgatg tttattttag ttactttgag cgtctggccg cacttgaaaa
      121 tgagatgatt attttcactt ctcccgattt gaaaccaagg gttgaagcga ttcgcaacgg
      181 aaaaccaaca acggttatcg ttatcgatat aaaaaagaaa tttagatata tcagaagtcg
      241 tatcgaaaaa attcaaaaag atgagtcgtt tacaaataga cttgagcctc gacaattaaa
      301 aaacccagag tactggtcac cagagtatgt tttggtatgt aaccttaagg cctattttgt
      361 aaataaagct atcaacatgg gcttagtgaa aacgcctctt gttgcgtgga tagattttgg
      421 ctattgccgt aagccaaatg taactcgagg attgaaaatt tgggatttcc catttgatga
      481 aagtaagatg catcttttca ctataaaaaa agggcttact gtcacatctc aacaacaggt
      541 ttttgatttc atgattggca atcatgtata tattattggg ggcgcaattg ttggttcaca
      601 gcataaatgg aaagaattct ataaattagt tctggaaagt caaaaaataa cactaaataa
      661 taacattgtg gatgatgacc agggcatttt tgtcatgtgc tattacaaac gccccgatct
      721 cttcaaccta aactatctgg ggagaggaaa atggttcgat ttgtttcgct gcttcaggag
      781 taacacttta ggggcaaaaa tgcaggcgct gagaattttc ctttcaagaa aataggtttc
      841 cctccattag tgggtagcgt tttgatacca actgagatag gaaaaatgat gctttggcat
      901 cattttttct ttttatatca atgcagtaaa atcaatcatc attatgccca gccaaaactg
      961 tacagttttt ctactatctg taggtgttga gagacatcaa gttacacgtc atttatcctg
     1021 aattttgcag aagagttaac gcgtcatctc gtcgcgacct ataagtttgg gtaatatgtg
     1081 ctggaatttg ccctgtctgg agaatcgcaa tgcgtggaga attttatcag cagttaacca
     1141 acgatctgga aaccgcacgg gcggaagggt tgtttaaaga agagcgcatt attacgtctg
     1201 cgcagcaagc agatatcact gtggctgatg gaagccacgt cattaacttt tgtgccaaca
     1261 actatctcgg gctggcgaat catccggatc tgattgcggc agcaaaggcg ggaatggatt
     1321 ctcacggttt cggcatggct tcggtgcgtt ttatttgcgg cactcaggac agccataaag
     1381 agcttgaaca aaaactggcg gcattcctgg ggatggaaga tgcgattctc tactcttcct
     1441 gctttgatgc taacggtgga ctgtttgaaa cgctgctggg tgcggaagac gccattatct
     1501 ccgacgcact gaaccacgcg tctattattg atggtgtgcg tctgtgcaaa gctaaacgct
     1561 atcgctatgc caacaacgat atgcaggagc tggaagcacg tctgaaagaa gcgcgtgaag
     1621 ccggtgcgcg tcatgtgctg attgccaccg atggtgtgtt ctcaatggac ggcgtgattg
     1681 ccaacctgaa aggcgtttgc gatctggcag ataaatacga tgccctggtg atggtggatg
     1741 actcccacgc ggtcggtttt gtcggtgaaa atggtcgtgg ttcccatgaa tactgcgatg
     1801 tgatgggccg ggtcgatatt atcaccggta cgcttggtaa agcgctgggc ggggcttctg
     1861 gtggttatac cgcggcgcgc aaagaggtgg ttgagtggct gcgccagcgt tctcgtccgt
     1921 acctgttctc caactcgctg gcaccggcca ttgttgccgc gtccatcaaa gtactggaga
     1981 tggtcgaagc gggcagcgaa ctgcgtgacc gtctgtgggc gaacgcgcgc cagttccgtg
     2041 agcaaatgtc ggcggcgggc tttaccctgg cgggagccga tcatgccatt attccggtca
     2101 tgcttggcga tgcagtagtg gcgcagaaat ttgcccgtga gctgcaaaaa gagggcattt
     2161 acgtcaccgg tttcttctat ccggtcgttc cgaaaggtca ggcgcgtatt cgtacccaga
     2221 tgtctgcggc gcatacccct gagcaaatta cgcgtgcagt agaagcattc acgcgtattg
     2281 gtaaacaact gggcgttatc gcctgaggat gtgagatgaa agcgttatcc aaactgaaag
     2341 cggaagaggg catctggatg accgacgttc ctgtaccgga actcgggcat aacgatctgc
     2401 tgattaaaat ccgtaaaaca gccatctgcg ggactgacgt tcacatctat aactgggatg
     2461 agtggtcgca aaaaaccatc ccggtgccga tggtcgtagg ccatgaatat gtcggtgaag
     2521 tggtaggtat tggtcaggaa gtgaaaggct tcaaaatcgg cgatcgcgtt tctggcgaag
     2581 gccatatcac ctgcggtcac tgtcgcaact gtcgtggcgg tcgtacccat ctgtgccgca
     2641 acaccattgg tgtcggtgtt aaccgtccgg gctgcttcgc cgaatatctg gtgattccgg
     2701 cgttcaacgc tttcaaaatc cccgacaata tctccgacga cctggcttcc atttttgatc
     2761 ccttcggtaa cgccgtgcat accgcgctgt cgttcgatct ggtgggcgaa gatgtgctgg
     2821 tttctggtgc aggtccgata ggtattatgg ctgcggcggt ggcgaaacac gttggtgcac
     2881 gcaatgtggt gatcaccgat gttaacgaat accgccttga gctggcgcgc aaaatgggta
     2941 tcacccgtgc ggttaacgtc gcgaaagaaa accttaatga tgtgatggct gaactgggca
     3001 tgaccgaagg ctttgatgtc ggtctggaaa tgtccggcgc gccgccagcg tttcgtacca
     3061 tgctcgatac catgaaccac ggcggtcgta ttgcgatgct gggtattcca ccgtctgaca
     3121 tgtctatcga ctggaccaaa gtgatcttta aaggcttgtt cattaaaggc atttacggtc
     3181 gtgagatgtt tgaaacctgg tacaagatgg cggcgctgat tcagtctggc ctcgatctct
     3241 cgccgatcat tacccatcgt ttctctatcg atgatttcca gaagggcttt gacgctatgt
     3301 gttcgggcca gtccgggaaa gttattctga gctgggatta acacgaaaaa gggctggtat
     3361 tccagccctt ttgcctgagg ataatctgtt aaatatgtaa aatcctgtca gtgtaataaa
     3421 gagttcgtaa ttgcgctgat cttttatata gctgttctca ttatctctct accctgaagt
     3481 gactctctca cctgtaaaaa taatatctca caggcttaat agtttcttaa tacaaagcct
     3541 gtaaaacgtc aggataactt cagaggtcgt cggtaattta tgatgaacag caccaataaa
     3601 cttagtgtta ttattccgtt atataatgcg ggcgatgatt tccgcacttg tatggaatct
     3661 ttaattacgc aaacctggac tgctctggaa atcattatta ttaacgatgg ttcaacggat
     3721 aattctgttg aaatagcaaa gcattacgca gaaaactatc cgcacgttcg tttgttgcat
     3781 caggcgaatg ctggcgcatc ggtagcgcgt aatcgtggga tcgaggtggc gacgggcaaa
     3841 tatgtcgctt ttgtcgatgc tgacgatgag gtctatccca ccatgtacga aacgctgatg
     3901 accatggcgt tagaggacga cctcgacgtg gcgcagtgca acgctgactg gtcttttcgt
     3961 gaaacgggag aaacctggca atccatcccc agcgatcgcc ttcgctcaac cggtgtatta
     4021 accggcccgg actggctgcg gatggggctt tcttcgcgcc gttggactca cgttgtctgg
     4081 atgggggttt atcgccgtga tgttattgtt aaaaataaca ttaaatttat tgccggatta
     4141 catcatcagg atattgtctg gacaacagaa ttcatgttta acgcgct
//
LOCUS       DAZFBR010000219         4168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFBR010000219 DAZFBR010000000
VERSION     DAZFBR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.221"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..778)
                     /gene="ygcW"
                     /locus_tag="ACHJZ2_004853"
     CDS             complement(<1..778)
                     /gene="ygcW"
                     /locus_tag="ACHJZ2_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4570794.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYL"
     gene            1097..2374
                     /gene="yqcE"
                     /locus_tag="ACHJZ2_004854"
     CDS             1097..2374
                     /gene="yqcE"
                     /locus_tag="ACHJZ2_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO4570795.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            2401..3879
                     /gene="ygcE"
                     /locus_tag="ACHJZ2_004855"
     CDS             2401..3879
                     /gene="ygcE"
                     /locus_tag="ACHJZ2_004855"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO4570796.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         1083 a          952 c         1011 g         1122 t
ORIGIN      
        1 ctaaataacc gccatcgacc actaataaat gcccgttgac ataattcgat gcctgacttg
       61 cgagaaacac ggctgcgccc attaaatcct gagtatcgcc ccaacggttt gccggaatat
      121 gatcaagaac gcgctgattg gtttctggat tactgcgtgt cgccagcgta atgtctgttg
      181 cataatagcc aggggcgata ccatttacct gaatattata ttgacctagt tcatcacaat
      241 aagctttggt gaatccggca agagcatgtt tagtagcaga atatgcaggt gaccattgtc
      301 cacctaagta agagaacaat gaacagatat taatgatttt accgcttttc tgcgggatca
      361 taatttttgc cgcttcgtag cttaactcga atgcggcggt caggttcaca tcaatcatcg
      421 ggtcccagtc ggcacgaccg aagtccagca ccttattcag cttacaaata ccggcattgt
      481 taaccagaat atcaactgta ccgaaacgct cacagcaggc agcgataatc ttctgcggcg
      541 cgccttctgc ggtgatatcc acctgcatga agtccacctc aacaccctga ttttcaatca
      601 tttcctttgt ttcgccgtta tctttgacaa aacttggaat aaagacattt gcgcctgctt
      661 tggccaacgc catggcaaat gcctggccta aaccgctatt cccgccggta acaattgcgg
      721 ttttaccttt cagggagaaa aaatccattg agaacgcatt gagagattcg attgacatag
      781 ttagctccaa tttccgtaag gcaaaaaaaa agaaaggtaa cttcccccgc gagagggaag
      841 ttacctttct cattatctct ggtaactgga gataattttt agcataagga cataactgct
      901 aatgtgatcg taatcacagt gtgatattcg tattatcaaa tagttttaaa aagcagaagc
      961 gtgattactc tcacacaaca cctctgcttt ccatgttatt tattatttca attactagag
     1021 acctgagaaa agtaatccac ctgaaagggc ggcttacttt tctctttttt ttgtctatta
     1081 caaatgggaa tacgatatgc aacacaactc atatcgccgt tggataaccc tcgcgataat
     1141 tagttttagc ggcggcgtta gtttcgacct ggcttattta cgttatattt atcaaattcc
     1201 catggcgaaa tttatgggat tcagcaatac cgagataggt ttaataatga gtacctttgg
     1261 tattgcggct attattcttt atgcccccag cggcgttatt gccgataaat tttcacaccg
     1321 caaaatgatt acttccgcga tgatcattac cggattactg ggtctgttaa tggcaacgta
     1381 tccaccgctg tgggtaatgc tctgtattca ggtcgccttt gcgataacaa cgattttaat
     1441 gctgtggtcg gtgtcgatta aagccgcatc gttgcttggc gatcatagcg agcaagggaa
     1501 aattatgggc tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg cagtgtttac
     1561 catgtgggtt ttttctcgct ttgcaccgga tgacagcacc agcctgaaaa cggtcattat
     1621 catctatagt gtggtttaca tcttgttggg gattctgtgc tggttttttg ttagcgataa
     1681 caacaacctg cgcagtgcca ataacgaaga aaaacagtca ttccagctta gcgacatcct
     1741 tgccgttttg cgtatcagca ccacctggta ttgcagcatg gtgatttttg gcgtcttcac
     1801 catctacgcc attctgagtt actccaccaa ctatctgacc gaaatgtatg gcatgtcgct
     1861 ggtggcggcg agctacatgg ggattgtgat caacaaaatc ttccgcgcgc tgtgcggccc
     1921 acttggcggc attatcacca cctacagtaa agtgaaatcc cctacccgcg tgatccaaat
     1981 cctttccgta ctcggcctgc tggcgttaac tgccctgctc gtcacgaact ctaacccgca
     2041 atcggtcgcg atggggattg gcctgatttt actgctggga ttcacctgtt acgcctcacg
     2101 cgggctgtac tgggcctgcc ctggcgaagc gagaacaccg tcttacatta tgggcaccac
     2161 ggtaggtatt tgctcggtga ttggattcct gccggatgtc ttcgtttacc caattatcgg
     2221 ccactggcaa gacacccagc ccgcagcaga agcctaccgc aatatgtggc tgatgggcat
     2281 ggcggcgctt gccatggtga ttgtctttac ctttttgctg ttccaaaaaa ttcgtactgc
     2341 tgatagcgcc cccgcaatgg ccagcagcaa gtaagccagg aaaattacag gagaaaaatc
     2401 atgtcgaaga aatacatcat agggattgat ggcggaagtc agagcacaaa agtggtgatg
     2461 tacgatctgg aaggtaacgt ggtttgcgaa ggcaaaggtt tattgcagcc gatgcacacg
     2521 ccagatgccg ataccgcaga acatcctgac gacgatttat gggcatcatt atgttttgcc
     2581 ggtcacgatt tgatgagtca gtttgccggg aataaagaag atattgtcgg tattggtctg
     2641 ggatccatcc gttgctgccg tgcgttattg aaagccgatg gcacgcctgc tgcgccgttg
     2701 attagctggc aggatgcacg cgtaacgcgt ccctacgaac acactaatcc tgacgtggcc
     2761 tatgtcacct ctttttcggg ttatctgacg catcgcttaa ccggcgagtt taaagacaat
     2821 atcgccaact attttggtca gtggccggtg gattataaga gctgggcatg gagcgaagat
     2881 gctgcggtaa tggagaagtt taatatcccc cgtcatatgc tgtttgatgt gcaaatgcca
     2941 ggcactgttc ttggacagat cacaccacaa gccgcgctgg cgacacattt cccggcaggg
     3001 ctgccggttg tttgtaccac cagtgataaa ccggtagaag ctctgggagc cggattactg
     3061 gatgatgaaa cggcggtgat ttcactcggt acttatatcg cgctgatgat gaacggcaaa
     3121 gccttgccga aagatccggt ggcgtactgg ccgattatgt cttctattcc gcaaacattg
     3181 ctgtatgaag gttacggtat tcgcaaaggc atgtggacgg tgagctggct gcgcgacatg
     3241 ttaggcgagt cgttaattca ggatgccagg gcgcaggatc tttcaccgga agatttactc
     3301 aacaaaaaag cttcttgtgt gccacctggc tgtaatgggc tgatgacggt gctggactgg
     3361 ctgaccaatc cgtgggaacc gtacaaacgc gggattatga tcggctttga ttccagcatg
     3421 gattacgcat ggatatatcg ttcgatactg gaaagcgtgg cgctgacgct gaagaacaat
     3481 tacgacaata tgtgtaatga aatgaatcac tttgcgaagc atgtgatcat tactggtggc
     3541 ggttcgaaca gcgatctgtt tatgcagatt tttgccgacg tgttcaacct tccggcacga
     3601 cgtaacgcca ttaacggttg tgcaagtctg ggggcagcga tcaatacggc ggtaggtctg
     3661 gggctatacc cggattacgc aacggctgtc gacaaaatgg ttcgcgtgaa agatatcttt
     3721 ataccgattg agagcaatgc caaacgctac gacgcgatga ataaaggtat tttcaaagcc
     3781 ctaaccaaac atactgatgt gatcctgaaa aaatcgtatg aagtgatgca tggggaattg
     3841 gggaatgcgg attcgatcca gagctggtcg aatgcgtaag tgaggaagac cgggcgggaa
     3901 actgcccggc ctgaacatac ctgaatggtt atccccgctg gcgcggggaa cataagttcg
     3961 acaacttaaa aaatgataac aagtccggtt tatccccgct gatgcaggga acataataaa
     4021 aactttaact agttcggttt atccctgttt ttacaaggaa tattaactac actcgatgtc
     4081 tttagaataa aagacaatac gaatcgcact ttatcaattt tcacttaaag tatgaactgg
     4141 atatctttat tcataagaac tattcatc
//
LOCUS       DAZFBR010000220         4105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFBR010000220 DAZFBR010000000
VERSION     DAZFBR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.222"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..901)
                     /locus_tag="ACHJZ2_004856"
     CDS             complement(<1..901)
                     /locus_tag="ACHJZ2_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4570797.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQHTASLSSPDTPRPL
                     SDSAFPDTLPGTEYGPDRGIRLSA"
     gene            complement(968..3075)
                     /locus_tag="ACHJZ2_004857"
                     /pseudo
     CDS             complement(968..3075)
                     /locus_tag="ACHJZ2_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            complement(3896..>4105)
                     /locus_tag="ACHJZ2_004858"
     CDS             complement(3896..>4105)
                     /locus_tag="ACHJZ2_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFO4570798.1"
                     /translation="LCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLKM
                     FLMMDMLGVGDAININTNKNIRQVC"
BASE COUNT          948 a         1159 c         1000 g          998 t
ORIGIN      
        1 ccgccgaaag gcggatacct ctgtcgggac cgtattcggt accgggcagt gtgtcgggga
       61 acgctgagtc tgaaagaggg cggggggtgt caggggaaga taaagaagcg gtgtgctgtt
      121 tacgggcctc ttccgcccgc tgcgcctgcg tggtcagcac cagacggaac tcccgcccgg
      181 caccgtccgt cacgccggtg atttccccgg ccaggtcacc ggcggcctca cgcctgtacg
      241 tcagcgtccg cccgaagcgg tccgccatcc cggtaagcac ccggtacggc ggcagcggcg
      301 ctggcagtac gtcctcagca cccggcaccc gctctgacca ccccagtatc caccacggcc
      361 cctgtgcgct gttggtcgcc aggtaaagat gcgggcttaa ccggatatcc ggcggcagcg
      421 ccccccacag ccgcgccagc gtgtggccgt ccggctgcgc tgccttacca ccgcgcacca
      481 gccacattga ctcgctgcgg ctgtacaccg cctcccccgg cagcagcggc tcaaagtgaa
      541 tgctccgccc gccgttgtca ttgagtacca gtgcatcatc gcgtagctgt aagcggatat
      601 cagaaggcgc tttccagccg gggccaaaaa tccccaccgg cgcaggcgtc cgggtccggt
      661 agctgctgta ggtgcgggag agaatgaacg gcagcgggcc gggcagcgca aggtccgtct
      721 cgccgggcag caccttcgcc cccagcagcg gatttaccgg gttgcccgaa gtcatcccgc
      781 ccgggcacac cgagcacgcc acgccagttg gcgcgccaat tcttacacct gccgaaccct
      841 ggacaatggg accgccatac tgagtcatat ctccctgacg cgccgctggt tttccgctca
      901 tgattcaatt ccttcttctg tttattttta atgcttgttt gttactgaat accgaacagg
      961 tacgggatca gtactctctc atatccggcg tatcctgctt ttcatcaggg gtcttcggtg
     1021 gggatttcga ggctgcacag ttccagttaa tcaacagtga gtcaccatta acctgctcct
     1081 taccctgcaa atgaattttt gtgccgttga gaaaaatttg tccgtccttc gtcagcacca
     1141 gccttgcctt accacagcag agctccagat gctcaccggc ggagtaaatc gcggtctgcc
     1201 cggtatcatc ctttttcgtt ttaccaacgg tgaaggtaac gttatttccg actttgacgt
     1261 cataacccag cccgaccctc aacgatttat gcaaaccgat ctgcgaggac tgcatcagag
     1321 ccaccgaagt gttcataatg ccacctaccg tcgtctggta cgccacgccc acggtcagcg
     1381 cacgaacaag tccaatcgtt tccacctgaa cagaacccac gttaatgatc tggttactgc
     1441 ccaccgtctc tatctggtta acgcccaccg tctgtatctg attgttggta accgcaatca
     1501 tctgattgtt gccaatggtc tcagcatggt tgtttatcac atccgtggtg cggttattca
     1561 gcacctcggt gttcatgttc ttctgcgcgt ggatgtagac ctgttctttc cctgtcgcat
     1621 cgtcaaactt cagttcatta aacccgctgc ccttatagtt ttcgaacgaa tggtcatctg
     1681 cgtcttcgtc cccggcaggc tgccgggtgt gcggttttcc tggtggtagg tacgccccat
     1741 aataatcggc tggtccggat cgccgttgag gaagtccaca atcacctcct gacccacacg
     1801 cggtatcgcc aggttgccaa agccggtgcc tgcccacgcc tgtgccacac ggatccagca
     1861 tgaactgtcc tggtttgacg ggttatagcg gtcccagtta aatttcaccc gcacgcgacc
     1921 atgttcgtcg cagaagattt cctcgcctgc cggtcccgtc acgacggcgc tctgcgggcc
     1981 atccaccagc ggtttcagca acggctgtgg tcgccatgtc cggtctgccg gtattaccgc
     2041 aaagtggtta ttcagcgtgg tacctgaacc cctgcgtccc ggcaccgcct gtggctgctc
     2101 gccgtgcagt tcacttgcca ccacctgcca ttcccggttc aggttcgcct gcggatgccc
     2161 cgtcagcaca attcgccgtc ccggccatat ttccggagaa cggcttgttc cgcgcgccac
     2221 ttctgcgttg ttgcgccagc catccatctg ccagcgggca aagttctgcc cgtgggcacc
     2281 cttgaaacgt ccggggtagt catacacttc atactgtgtg cgctggtaat cctggtactg
     2341 gccttcctga tcaaaacgcc ctgcccagcc gggtcgttta aaggtgtagt ccttggtcac
     2401 cacggaagaa gggcggattt gtgcgctgta gcggaactgg ctgatgcata gggtgctcac
     2461 ctcggtacgg gtgttcgggt tccaggggat ctcaaaggac tccggcagat aacgcacggt
     2521 gtcgcacagg accaggctct ggtcgatact tttttgcgcg tgctcctcat aaaagaagat
     2581 gccttcctcc gccgccatcc ggcacaggaa atcgtaatca gtttcgccgt actggacaca
     2641 aaactcacgg gaaggatgcg gctcgctgaa cagcggactc cactcggtca caccgttttc
     2701 tttcaggatc gtagcgagta tgctttcgat gtcctcattc tggaagatac ggaagttctg
     2761 gcgcagccct gtgcgccaca gcggcgggca caccttcatg ctgtacagca tctggttttt
     2821 gtcgttctcc cccagttcaa accaggtcac cacacctttc acccggcgct gtacgtcatc
     2881 gccctgccat accgtcaggt aggccatttt gtccagcacc tgcgcaaatt caagggaaag
     2941 aaactgctga ctgaccagag aaaggtcgag ggaaaaaagt gaagagagtg actggttcag
     3001 atgaaaggag accaccgcaa acgcgtccgg tggcaggccg tccacttcca gcgtgaaacg
     3061 taatccggtt gacatagcaa cctccgtgaa aatgaataat aatccgcaca tattgcatgt
     3121 acaacatgtc gggcggatga aataagtgag tcgacatctg gcgttaccgg ggaatatcag
     3181 aaaaatgtca tggttatgtg atgatttagt ctgggatttt acttcagaga aacgggtgtc
     3241 tgtttagtca acaaaataat cacaaacgca acggatgggt tctcgacgtt attcacgaca
     3301 ggctcgtaca ttacagcgag taataaaaaa cctgatatgc aaagtcaaac caatattcat
     3361 aatgtctttt tattcacaac aataaataat ttaataacaa tcattgaaat gaatgatgat
     3421 ttgtataagg aatatacgct accgccccac cctgataaca tgatctgcac attgccatct
     3481 taacgtcgtt aaacactggc ttaatagtgg atatttcaaa ttaggaattt attaaattca
     3541 cctgatgaat atcataagca aatagcatag aaaataatga caactccccc gaggggacca
     3601 tttccaaagc actgcaccac cacctggtgc ccggatgatc ttgcttcagt ggtaagagta
     3661 cctgattcat tgttcccaaa atggtgtttt cccctgcccc tccaataatg tgcagctctc
     3721 acgtgataat ctattgatta ggttatatta agaatcaata tttaaaatac atccattttc
     3781 ttgaataaat caacatgttt attaatcaat tgctgtttta gctctgactc atcaagctct
     3841 ccatatttgt cctacagata attgcgaata aatacagaga acgccattta tccttttagc
     3901 atacctgccg gatattttta tttgtattaa tattaattgc atctccaact ccgagcatat
     3961 ccatcattaa aaacattttg agattataga atacctgtga taacaagtga aacatctgca
     4021 caatgtgaat agttagtttc ttttcagagg aaatgaaata gacaagctga atgaaaggga
     4081 gtgtgatata aattaaaaaa cataa
//
LOCUS       DAZFBR010000221         4104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFBR010000221 DAZFBR010000000
VERSION     DAZFBR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.223"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(93..761)
                     /gene="artM"
                     /locus_tag="ACHJZ2_004859"
     CDS             complement(93..761)
                     /gene="artM"
                     /locus_tag="ACHJZ2_004859"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO4570799.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(761..1477)
                     /gene="artQ"
                     /locus_tag="ACHJZ2_004860"
     CDS             complement(761..1477)
                     /gene="artQ"
                     /locus_tag="ACHJZ2_004860"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO4570800.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1484..2215)
                     /gene="artI"
                     /locus_tag="ACHJZ2_004861"
     CDS             complement(1484..2215)
                     /gene="artI"
                     /locus_tag="ACHJZ2_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO4570801.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2233..2961)
                     /gene="artP"
                     /locus_tag="ACHJZ2_004862"
     CDS             complement(2233..2961)
                     /gene="artP"
                     /locus_tag="ACHJZ2_004862"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO4570802.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3179..3694)
                     /locus_tag="ACHJZ2_004863"
     CDS             complement(3179..3694)
                     /locus_tag="ACHJZ2_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4570803.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLSQSIENR"
BASE COUNT         1049 a         1059 c         1016 g          980 t
ORIGIN      
        1 cgcatccggc acaggcaccg tgctgatgtc tgatgcgacg ctggcgcgtc ttatcagacc
       61 tacaaaaccc ccggcgaatg tacgcagcca cattaattcc ttcgctcaaa cgccagcgct
      121 ttgcgctcga tcagacgcat catcagcgtc agcaggccgt taacgaccag ataaataatc
      181 cctgccgcgc cgaacaccat gacatcgtag gtgcgtccgt acaacaactg gctgtatccc
      241 atcacttcca tcagcgtaat ggtgtaagcc agagaggtac ttttgaacac cagcaccact
      301 tcgttggaat aagaagagag cgagcgttta aaggcatacg gcagcaggat cgccagcgta
      361 tcttttttgc tcattcccag ggcgctacag gactgccact gaccttccgg gatcgcacga
      421 attgcaccgt aaaacagctg cgtggtatac gccgcactgt tcagcgacaa cgcaatcagc
      481 gcacataacc acggttctga caacaaatgc cacagtgccg gatactcctg caacgtcggg
      541 aactgacccg gcccgtaata aatcaggaag atctgcacca gcagcggtgt accggtaaac
      601 agcgtgatat aaccccgcac cagccacacc agcaccggcg ttttcagcgt caggatgatg
      661 gtaaaaatca acgccagaat cagtgccaca atcagcgagg caacggttag cgtcaggctg
      721 gtgtgcagcc ctttcatcag ttcgggtaaa tactcaaaca ttagctgggc ctccgctcaa
      781 aacgtgtcgc gcgcaggtca atgcgtttga gaatgtactg actgagcagg gtgatcacca
      841 ggtaaatcgc cgccgccaca atgtaccagg taaatggttc ctgggtacga gtagcgatgc
      901 ttttggtttg cagcattaaa tcattcacac taatcaaact gaccagcgcg gtatctttca
      961 gcagcaccag ccactggtta ccgaggccag gcagcgcatg acgccacatc tgcggcatca
     1021 ccagacggaa aaagatagcc gatttcgaca gccccagcgc ctggccggat tcccactgac
     1081 ccatcggcac cgctttcaac gcgccgcgca gcgtttgcga ggcataggcg gcatacagca
     1141 gtgacagagc gatgacacca caaaggaacg ggctaacgtc gaagttttca atctgcatct
     1201 gcaccgggat ctctacaatc ccaagattga tagtgaagcc atccgaaagt gtcagcagca
     1261 gctgcgagga gccaaaatag ataaacagca ccaccagaat ttctggcagg ccacgcagaa
     1321 tggttaccag cgctgaacct gcccacgcga caggacgcca tttcgcagac tcccataccg
     1381 caaagaacat cgccagcgcc agcccgacaa tcaatgcaca aacggcaagg ccgacggtca
     1441 tcccggcggc gcttgctaaa ggaaaaaatt cattcatcag gaattacttc tggaaccatt
     1501 tgttgtagat ggtttcgtaa gtgccatctt tcttcacttt ttccagcgca gtgttgagtt
     1561 tctgctgcag ctcagtgttg ccctgacgta ccgcgatgcc gaggccagta ccgaagtaat
     1621 ctttatcggt cactttatcg cccactgccg ccagtttcgg gttatctttc agccattcag
     1681 ttaccactgc ggtgtcacca aatacgctgt cgatacgacc gttttgcaga tccagttttg
     1741 cgttctggta gctgtcatac ggcacggtgg tgatttccgg gtgcttatcc ataatgaatt
     1801 tctggtgagt cgtaccattc tgtacgccga cttttttgcc tttcagctga tcaacgctgg
     1861 tgtatttacc ctgctgaccc acaaacaggg cagagttgtc atagtacggc gtggtaaaca
     1921 gtacctgctt ttcacgttcc gcggtgatat ccatcccggc catcaccgct tccacgcgac
     1981 ggaatttcag gcttgggatc aggctgtcaa atgcctggtt agagaaagta caggtcgcat
     2041 caatctcttt acacagcgcc tgtgccaggt cgacgtcaaa accaacgatc tggttgtttg
     2101 catcaatcga ttcaaacgga ggataggagg cttcggtagc aaaacgaatg gtttcggcag
     2161 ctgtggcgga aagactaaaa cctgcaatta acgcggcaat cagaactttt ttcattgttg
     2221 ttatcccgaa tcttagtgag agagatagtt tttaaatgct tcggtttgcg gctcggtaaa
     2281 gcagctcgcg tcgccttgtt ctacgatatg accgttttcc atatacacca ctcggctggc
     2341 ggttttacgc gccacttcaa cttcgtgggt gacgatcacc tgggtaatat tcgtttctgc
     2401 cagctcacga atgatgctga cgatttgtgc cgtaatttcc gggtccagtg cggcggtcgg
     2461 ttcatcaaac agcagtacct gcggttccat cattaacgca cgggcaatag caacacgctg
     2521 ctgctgacca ccagaaagat gcagcgggta acgatcgcta taaggtttga gacgcagacg
     2581 ttccagcagt ttttctgcac gggccagcgc ctgatcttta ctcaacccca gtacacggca
     2641 gggcgcttca atcaggtttt gctgcacggt cagatgcggc cacaggttgt attgctgaaa
     2701 caccatgcca acgttacgac gcaaatcgcg aatcgctttg tctgagggtg ttttggtgaa
     2761 atcgaaatgg ttgcctgcaa tgttgagcgt accggagcgc ggcatctcaa gcagattgag
     2821 tacacgcagc agcgagcttt tacccgcgcc gctggggcca agtaacacca gcgtttcgcc
     2881 ctgtgggcaa tccagcgtga tatcgaacag cgcctgatgc gcgccgtaga agcaattaat
     2941 gccgtttaat tgaatactca ttgacactcg tatactggca gtctgatagc tattgaggtc
     3001 gaagatagta cctttgacag aataattatg caatatttct gctttaaaag ttaaaagcaa
     3061 agcgcattat tcaataaaca tagcacaaaa taacgggggc ggtggtcggc gagcataaat
     3121 gtcggcattc ctcacgaaat gccggacaat ttacggggtt tattggttga tcaaggcgtt
     3181 agcgattctc gatggactga ctgagcgtac ccgccgtggc atgaacgcta ccgcctaagt
     3241 agcgcacatc gtcgatcacc caacactggc cttcctgaat cattaacact tcatcctgcc
     3301 agccctggtc accctgcttg agatccacgc gcaatggaat gttacgggca tcacgattag
     3361 ggatagtcga tgcactggca acgtgggcgc tgtctggcaa ggtggttcga ctggagaacg
     3421 gatcactgct taacaattca cgatgactat tatcgcggct ggcatcgcta agtagggttg
     3481 ccagtttgtc actcaaatag gggcgcaggg cggtaatgtc attgctgcga tgttgaatgt
     3541 ggtagtcata gaattgttgg gccacgctgt caggcccccc ctggacacag gaaccggtgc
     3601 ggggaccatt atctttataa gctggcgtga cggtggtaca ggcactcagc agcagtgcac
     3661 atggaataag tagagatagt gaacggtagc gcataatcat ttccttatta gcttgctgtc
     3721 tgcgtcacac tacgaccaaa cgctatcaga ttatttaata cattgttttt aatacaattc
     3781 tgaaacaatc tgaacacctg attgggtttg agtccattgc tggcgaccca ctcaaggttg
     3841 ctcgtagctg acttacttaa attacccaaa caatagacag tgtaaaccat ttgtgaggtt
     3901 gagcgtgtta tatggctgaa atgatcattt aaggtaaata tgatagttct gaaatggtgg
     3961 tataaatcct cctgtagagg ttaagagcat ctgcaagaag ccagtttcac atttataacg
     4021 cgtgataaat gaacgcggtt attgtctaat cattacaaat ttttcacatg cgataagaga
     4081 tcctgctatt aagatgattg ccaa
//
LOCUS       DAZFBR010000222         4096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFBR010000222 DAZFBR010000000
VERSION     DAZFBR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.224"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHJZ2_004864"
     CDS             complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHJZ2_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4570804.1"
                     /translation="MKQQKAMLIALIVICITVIVTALVTRKDLCEVR"
     gene            complement(345..449)
                     /locus_tag="ACHJZ2_004865"
     CDS             complement(345..449)
                     /locus_tag="ACHJZ2_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570805.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(565..1152)
                     /locus_tag="ACHJZ2_004866"
     CDS             complement(565..1152)
                     /locus_tag="ACHJZ2_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4570806.1"
                     /translation="MTTITKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AVSDERAAYELFMEKRFGESVDRRRAKNGDRDYMVWDMALGWIIWCHRAAMLQAGNFR
                     ENKGSSTNNFREISETSTNYPVTPDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDG
                     EFFDVVRNGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            complement(1155..1346)
                     /locus_tag="ACHJZ2_004867"
     CDS             complement(1155..1346)
                     /locus_tag="ACHJZ2_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570807.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDMPVELKLRNVEIKVK"
     gene            complement(1348..1785)
                     /locus_tag="ACHJZ2_004868"
     CDS             complement(1348..1785)
                     /locus_tag="ACHJZ2_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4570808.1"
                     /translation="MGKIDYQALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            complement(1772..2089)
                     /locus_tag="ACHJZ2_004869"
     CDS             complement(1772..2089)
                     /locus_tag="ACHJZ2_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570809.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYIADEVNDLAILALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     GQD"
     gene            complement(2043..2360)
                     /locus_tag="ACHJZ2_004870"
     CDS             complement(2043..2360)
                     /locus_tag="ACHJZ2_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO4570810.1"
                     /translation="MNIDPAITIDMALNAGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQEQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPH"
     gene            complement(2350..2652)
                     /locus_tag="ACHJZ2_004871"
     CDS             complement(2350..2652)
                     /locus_tag="ACHJZ2_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO4570811.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKTEEVLKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGAGAELALEEKPGHAVIFQEVGSEY"
     gene            complement(2649..2966)
                     /locus_tag="ACHJZ2_004872"
     CDS             complement(2649..2966)
                     /locus_tag="ACHJZ2_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4570812.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELAWQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            complement(2963..3679)
                     /locus_tag="ACHJZ2_004873"
     CDS             complement(2963..3679)
                     /locus_tag="ACHJZ2_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4570813.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGGLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTEAKADDLILPSLHVANRELRRAKSNVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            complement(3713..>4096)
                     /locus_tag="ACHJZ2_004874"
     CDS             complement(3713..>4096)
                     /locus_tag="ACHJZ2_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4570814.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRDRGIERQMTEGELKRLAERQLTKWAKQVGNGMSIPPIRRQLASP
                     KCPQGPTPIELLKQEYERRKAAGFV"
BASE COUNT          925 a         1211 c          843 g         1117 t
ORIGIN      
        1 tcgtacctcg cagaggtctt tcctcgttac cagtgccgtc actatgacgg tgatacagat
       61 gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt
      121 cggcgcgtaa gaggctaacc tacatgtgtc tagcatgaaa ttggcctcag attaatgtta
      181 ggcgtcttgc aggacgcgta atgttaactg gggcttttct ctgtctacct tacggtggca
      241 tgcccgaggc agacagcctc aagcacccgc agcaattcta cttaactctc gctttaccgc
      301 aaaccgtttt tacccgatat gggaattccc atatcgtaat gaattcagtt ccctagtcga
      361 tccatcaaaa acacaaccag gcagtaaacg cccacaacag caataacagc cagcgcacct
      421 tccattgcca gtgaaatatc atccgacata ttccctcctt tggtgtgaat cccggcgaac
      481 gtttttaccc ccaccgacaa ataacatata ctaaaaaatc aatagctata gcaacgcctg
      541 taattgcaaa ggcttcaggc cagatcattg gcgcacctcc tgcggcggtt ctggtagcgg
      601 catccagtcg gttacattgc ggctctgtgt ttcgaaaaat tcatcaccat tacggactac
      661 atcaaaaaac tcaccgtctc gatattgcgc ataaagaacg aatgcgccat cacataaaat
      721 aattacgtgc tgaccatcat ccggcattcg ctcactacag cttatccaac catccggagt
      781 taccggatag ttggttgacg tttccgagat ttcccgaaaa ttattggttg acgaaccctt
      841 attttcccga aagtttccag cctgaagcat ggcggcgcgg tgacaccaga taatccagcc
      901 aagcgccata tcccatacca tgtaatctct atcgccattt tttgctctgc ggcgatctac
      961 agattctccg aaacgcttct ccataaataa ttcataggct gctcgttcat ccgatactgc
     1021 tgccagtgat gccagcgcaa tccgtgccag ctcttccgct tcttctgctg gaagcacaac
     1081 gttgctaccc ggtccgtatg tttcgcgcca atgctggatt gtcagcagtc gatctttggt
     1141 aatagtggtc atagctattt caccttaatc tcaacatttc gcagctttag ctctactggc
     1201 atgtctgact ttcctgttaa tgctaatgcg agattttctg gagtaatgag agcagttatt
     1261 gttttcccca tcgccagacg aataatcatt cgtatttcgc gatcgtcaca tgctcctggt
     1321 cgaacaattg atatttgtcc gttcatctca ctctcctttg atgcgaatgc cagcgacgcg
     1381 taatgcgtgt tctaggtcaa tcaggtaaag ccaactgcca ttttctttag gtatcatgac
     1441 atgtcgctca tctgcattta tcgggtgtcc atatcgaagg tcgtagcgag tcggtaattg
     1501 aacttcccgc gcttccagtt cagcaatacg cttgctccca tcagagataa cgccttcgta
     1561 atactcacgc tgctcgttga gttgtgattt tgcttcttcc agtccatcca gcaaatcagc
     1621 gataatatcc gcttcccgat gacggatgtg acgcttaaac gcagcaagag ccgcatcaca
     1681 atcccgttca gcatttgggc tgtccggggt agcctgatac cacgccagcg tcgattgata
     1741 gttttgtgct gcctcacgca gtgcctgata gtcaatcttg cccactggct gcctcctttg
     1801 ccgggatttc taacttttga gtggttgtat caaattcaaa caacttaacc acgtcatcaa
     1861 acaggacata atcaccatca ggatcttcag tcatatcagc gccacaatcc tgaccgcacg
     1921 agtcgcaacc tcccatatca agctcgtatc gcttcaggtt cgcgatattt gataaattca
     1981 gcgccagtat agccagatca ttaacctcgt ctgcgatata ccctgcacca tgtccataca
     2041 tttcaatgcg ggatatgatt tcttctacac gttgttttgt gatcgtcatt tttctcttca
     2101 ctccgatata caaggattac tacaccccct ctgctgattg cgcgagctgg atcccctggt
     2161 tccatgccgt caattccgaa ggcttcgaaa aatgcatcca ttgccttctg gcgttgctcc
     2221 tgcttacggc gtttattcca ttttttcatg aacaacagtg acagccatcg tccgctgcag
     2281 aacataatgt agaaataacc aagaagtgcc aggccggcgt tcagggccat atcaatcgtt
     2341 atcgccgggt caatattcac tgcccacctc ctgaaaaatc accgcatggc ccggtttctc
     2401 ctccagtgcc agctcagccc ctgcacctgc cgactgctgc cagcctttca gcatgtaaac
     2461 cgcatccacg caacggagca ttgccatgca aatatccatg tagtgtggct gagtcagccc
     2521 gtccggaagt actgccgggt ttaaaacggt atgcccttcc cgtttcagca cctcttccgt
     2581 tttgtgaaat gcctcgcggt taaaattttc atatcccgtc atcggaccgg cgatataaac
     2641 cctcaccctc actcctgaac cctcctgtcg aaataaacgt agttattcac tgcgcccaac
     2701 ttcatcccaa acttttcggc aatttcccgt cggggtacac cacgctgatg cagttgccac
     2761 gccagctcaa tatcacgctg tgaacatttg gctgactggt gataatcacc ccgtaacatc
     2821 atgctgatac ccagttcccg cgctttcgtc ctgacggctg actcactacg gccaatcaga
     2881 taaccgatgc tttcgactct catcgttccc gcacactgcc ggagtatcag gatttcagcc
     2941 cagcgccact tcttccagcc actcaccgct gctgctctct ggtggcggta atatcccgga
     3001 gaatatccct gtgtttgttc agttcccgta gcgcagcaca gactcgctcc cacttctgaa
     3061 cattactttt cgcccggcgc agctcgcggt tagccacatg cagcgatggt agaatcaggt
     3121 catctgcttt cgcttcagtg aacgattgct gcaacttcac aatgtcctcc accgcttctg
     3181 ttttaatttc ttcctgtgca gccgtttcct ggactggtaa cgcaacacca gctggctgag
     3241 gaagggcttt accctccgtt tcagctacgg atgctgcttt cggctctgcc ggtaaatcaa
     3301 cgcccggtat gcagtaacga aatttaccat cctgattcac gcgaatcaga ccccctttgc
     3361 tgattgccat tgccagcgat gaattcgccc ggcgggaggt aattccgaac atcaatgcca
     3421 gttcatccgc cgtttgtggg ccatgttgtt caatcgcctc tgtcagcatt tgcgccgtca
     3481 ctttcggtac cggtgacacc ggttcacttt caccagcctg agtcagccac catatcgccc
     3541 ccttgttatc cgcttctcca cggcgtttca gtttccacag ttcgttgaca gcctcttcac
     3601 gactaattcc aaggcgagct gccaccacat gtgaagaggc tcttttcagt gctttcagtg
     3661 cgtcagatac ggtttccatt aaaatttcct ccgacaaaat cgtttctcag attcaaacaa
     3721 aaccagccgc cttccggcgc tcgtattcct gtttcagtaa ttcaattggc gttggccctt
     3781 gcgggcactt cggcgatgcg agttgtctcc ggatcggggg aatactcatc ccgttaccaa
     3841 cttgctttgc ccatttcgtc agttgccgtt ctgcaagtcg ttttaactca ccttccgtca
     3901 tctgccgctc aatccctctg tcacgcatct cgaggcaaat gtggtacagc accggctgtg
     3961 gccacgggta tttatcactc ccgtcgtacc gccaggattc attcctccag cgacggtact
     4021 cctccatcac ggcatccacc gtcaggccaa atggatttgc cccgctctcc gaaatcaacg
     4081 ccacaaactc agccag
//
LOCUS       DAZFBR010000223         4014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFBR010000223 DAZFBR010000000
VERSION     DAZFBR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.225"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..106)
                     /gene="escP"
                     /locus_tag="ACHJZ2_004875"
     CDS             complement(<1..106)
                     /gene="escP"
                     /locus_tag="ACHJZ2_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle length
                     regulator EscP"
                     /protein_id="HFO4570815.1"
                     /translation="MTRVSLKRNLIEPVSFRPSDDWPQGEESFNDIFYH"
     gene            complement(99..419)
                     /locus_tag="ACHJZ2_004876"
     CDS             complement(99..419)
                     /locus_tag="ACHJZ2_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO4570816.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKENLQHVNKSVEKLSFAIKE
                     HYFD"
     gene            complement(479..1819)
                     /gene="escN"
                     /locus_tag="ACHJZ2_004877"
     CDS             complement(479..1819)
                     /gene="escN"
                     /locus_tag="ACHJZ2_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO4570817.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1803..3830)
                     /gene="escV"
                     /locus_tag="ACHJZ2_004878"
     CDS             complement(1803..3830)
                     /gene="escV"
                     /locus_tag="ACHJZ2_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO4570818.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSAALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMLVMAIIPGFPALVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNKTN
                     QQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFLKNIMNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            complement(3827..>4014)
                     /gene="cesL"
                     /locus_tag="ACHJZ2_004879"
     CDS             complement(3827..>4014)
                     /gene="cesL"
                     /locus_tag="ACHJZ2_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFO4570819.1"
                     /translation="AFFSFFSAFAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFFLMN
                     TFQQHVQIIERVRRMTS"
BASE COUNT         1237 a          919 c          727 g         1131 t
ORIGIN      
        1 tatgataaaa aatatcatta aaactttctt ctccttgcgg ccaatcatct gatggccgaa
       61 aagaaacagg ctctatcaaa tttcttttta gagaaactct agtcaaagta atgttccttt
      121 atggcaaaag ataatttttc tactgattta ttaacatgtt gcagattctc ttttgttcgt
      181 ttttgtgaat ctagcaatga acgcttttcc ttcgaaagtg aagatttttt ctcatacaat
      241 cgctgctttt gctcatcaaa tgcattgttt ttaatctgat atttatacaa cgacacttta
      301 tccaaattca ccagcgatgc acaatatgcc tgcttagagg caatcgactc tttaatggat
      361 tgctcgcagt catcgagttt accatccacc tcttttatct gagagttaat cttagccatt
      421 gattctctca atcggttcgc tctgctttta cgaatagata aaattctgtc caacatactc
      481 aggcaaccac tttgaatagg ctttcaatcg ttttttcgta actactgata tctttggtgc
      541 tttgttgtat aaagttctga attaatttcc tattttttat tgccttatct gcttcaggat
      601 cttgccccat cgtgtactca ccaatacgaa taagcagctc aacattttta taagttgcaa
      661 taagcttttt gcattctgcc gccgcacgca aatgctccga tgtaacaacg ttatgcataa
      721 cacgactggc acttaaacca atatcaattg cagggaaatg gttttcctct gcaagttctc
      781 gggtaagtac gatgtgccca tcaagaatag agcggacttc atcgccaata ggatcattaa
      841 cattatcgct ttctaacaac accgtataaa tcgcggttat tgaacctttt ggcgcaggcc
      901 ccgctcgctc taataatttg ggtaacgaag aaaaaacact cggagggaat ccccccctta
      961 catcaggttc tcccgatgca agcccaacat cacgtgcggc tcgggcataa cgagttacag
     1021 aatccatcat taataataca tttttacctt gatcgcggaa gtactctgca atagtcgtcg
     1081 ccgtaaatgc ggctttcatc ctttccagcg cggggcggtc tgacgttgtg acgaccagta
     1141 cacatttaga aagcgtggat tgaggtaaga gcgcgaggaa ttcatttact tcgcgaccac
     1201 gttcgccgat aagagcaagg acaataatat ctgcggatgc gccattacaa atcatcccca
     1261 aaagcgtact tttaccaacg cctgaaccag caaaaatacc gatacgctgc ccaataccgc
     1321 aagttagtag cccatcaatt gccctgacgc caagtataaa tggctgatca ataacctgcc
     1381 ttaataaagg atctggtggt tcagcataca agctgcgttc aaacggaaga tagggggcag
     1441 caatattact ctccattggt ctgcctatgc catcaacaag ccgcccaagt agtgcgtctc
     1501 caacacgaat cttgaactca tctccctgat aacttaacca ctgaccacag tacatgccgg
     1561 aaacatgctc aaaaggcaac aagaaaacct catcttcatc aatagcgatc acctcggcta
     1621 aacgttgact gggttctatc ttataaaagg cgccaatacg cgcttttggt aaacgcgcct
     1681 taataatcgt cccgccgata ttgataatct tgccttcata tctggtagac gaatttaatg
     1741 ataatgccgg cacagtgcta ttgagcactt tctgaatacg tgggtatttt tccaatacag
     1801 aatcatgctc tgaaatcatt taccgttcct aatactttaa gttcgatatt attcccaacc
     1861 tcctgaaaag agagcacggg gactgacggg aacgaacctt caatcatttt cttcacatag
     1921 cgcctgatat ctaaagcggt cagaatgacg ccgttccccg ttggattcat gatatttttt
     1981 aagaagccaa ttatctgctc agttcgctcc ggtgagatat tcagatacga acctgatgac
     2041 gtttgtctga tcgactctcg tagctcattt tcaatatcag agccaataag ccaaacgttc
     2101 agaagtgtac cgctaacgct atagcgacct aaaatatgcc gacgcagggc aatacgaacg
     2161 tattcgcaca agataaccac atccttttct tttgttgacc aaaaaataag cgtctcaaag
     2221 atagttctca ggtctctaat tgagacattt tcctctacga gacgttgtag gatgtcaaca
     2281 attttgctca aaccaagctg gcgctgcagc tctttcacaa gctcgttata ttttctctcc
     2341 atgatgtcca tcaaataacg cgtttcttgt acgccgatga actctttggc attagacaat
     2401 acaagtttct ttagcaaaaa cgttatctta tcatttgttg ttgcataaac atccatacct
     2461 gtaaggtgag cgagctgttg attagtctta ttcgtcttat atacgataga gttagtcgaa
     2521 agcttttcct ggacaaattc gaatgatatc tctgcatgcc cagcctcaaa atagacagta
     2581 tcatctataa gagtatcttt gtatattgac tcttgataga gcaaaaattc tattgcgcta
     2641 tcattatttt taaccggatt aacaataatc ttaggcaatg gaattccact ttcctcaaag
     2701 aaattccaac gcatgacctc aatttgctgt gtaatgtctg aactataaat attactactt
     2761 aagtttaaaa tcaatggctc agcgcccggt gagatgtttt ggctattaga caaatcagct
     2821 cccatagcat ccttattatt gccagttcca aatgttcttt tcttctgtaa tttccaggct
     2881 atccccaaca ggcaaaccgc cagaaataag aagaccaatg caggaaagcc cggaataatc
     2941 gccattacga gcattagtgc ggcactgatt aaaaaagggc gactattaac agaaacctgt
     3001 tgaacaatat cacctgcaag gttttcttct ttttcgcttt caccgggcac acgagtaaca
     3061 accacgcctg cggtgacaga aataataagc gcagggatct gggcaaccaa agcatcaccg
     3121 acagacaata cagaaaacag gctaagtgcc gcactaaatg gcatgtcaaa ttgccacata
     3181 ccaatgagca cgccaccaaa aaggttaact aaaacaataa tgataccagc aatagcgtcc
     3241 ccttttacaa acttcatcgc cccgtccatt gaaccataaa actggctttc cttttgcact
     3301 cgggagcgaa gaacttttgc ctcaagcggg tcaataacgc ctgcgcgcat atcaccatca
     3361 atactcattt gctttcctgg cataccatca agggaaaaac gagcactcac ttcagcaaca
     3421 cgctcggccc cttttgtaat aaccataaat tgaacaatgg tgataatagt aaaaataacc
     3481 aggccaacaa caatattacc gccaacaaca aagttaccga aggagtaaat aatgtcaccc
     3541 gcatcgtgat gcagcagaat aagtctggtt gtactaacac tgagcgacag gcgcatcaac
     3601 gtcgtaatca acaggattgt ggggaaagaa gttagttcaa gaggattttt tatatagatt
     3661 gagagcatta ataaaagtaa agctgtcgaa atattaatcg cgataatgat atccaccaca
     3721 actgttggta gtggaataat catcatcatt acagccataa agaagaagag agccagaata
     3781 agatcgtgat atgactctgc ttttttaaat atatttaaga gtttattcat gatgtcatcc
     3841 tgcgaacgcg ctcaataatc tgcacatgct gttgaaaagt attcattaga aaaaaaacat
     3901 cgatattggt taacaaacaa cttaagcaca atttattatc gtagacaaaa atatgttcaa
     3961 tcactccctg cgtacgctca ggagcaaagg cactaaagaa agagaaaaat gcgt
//
LOCUS       DAZFBR010000224         4010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFBR010000224 DAZFBR010000000
VERSION     DAZFBR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.226"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..979
                     /locus_tag="ACHJZ2_004880"
     CDS             <1..979
                     /locus_tag="ACHJZ2_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4570820.1"
                     /translation="AGCRTTASLNITDGINVGEILANETSFSKSVVFTGISCDTSTDK
                     IVYKNIQSDWVEVGPFGNGEKLKVKIESLGKTSDTIGKSSNAQAVLPYVVKIARGTPD
                     FTGERKSTWFISDTVIANIGGESSSSIDFWLGICKALKFNWCVNYLTSKLAGDTFTLG
                     LNISYYPKNTTCKPENTVIKVDDIALFQLRNQGKIAANSKEGTITLKCDNLFGDKKQA
                     SRNMVVYLSSSDLVKGSNTILRGKTDNGVGFVLDLTEPPKGTEAAIKISANGDQGAAT
                     SLWKTDKPGVSLNSNIINIPVMASYYVYDEKKVKSGALEATALINVKYD"
     gene            complement(1061..1399)
                     /gene="rcnB"
                     /locus_tag="ACHJZ2_004881"
     CDS             complement(1061..1399)
                     /gene="rcnB"
                     /locus_tag="ACHJZ2_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO4570821.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1617..2456)
                     /gene="rcnA"
                     /locus_tag="ACHJZ2_004882"
     CDS             complement(1617..2456)
                     /gene="rcnA"
                     /locus_tag="ACHJZ2_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO4570822.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHEHHHHHEHGDNEEYQDAHAR
                     AHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSF
                     SIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMG
                     IMR"
     gene            2577..2849
                     /gene="rcnR"
                     /locus_tag="ACHJZ2_004883"
     CDS             2577..2849
                     /gene="rcnR"
                     /locus_tag="ACHJZ2_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO4570823.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            complement(2959..3273)
                     /locus_tag="ACHJZ2_004884"
     CDS             complement(2959..3273)
                     /locus_tag="ACHJZ2_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFO4570824.1"
                     /translation="MKKLLVTVIFVAMMPNAFALSHGDSATLKEGTFNCKKLTDFYEM
                     ISYIQDNDQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGGWAVKQFF
                     EN"
     gene            complement(3283..3630)
                     /locus_tag="ACHJZ2_004885"
     CDS             complement(3283..3630)
                     /locus_tag="ACHJZ2_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570825.1"
                     /translation="MKSKIFAALLLLPLSTYASSYKCDALQQGQIGSNGKTVTAQSSI
                     DIDGKQMHFSLNGVSWDLSYIGEKQIAKMYSTESGNVSVSFIDANPIIFVLHLVNDDG
                     RKGTYNISECSEL"
BASE COUNT         1148 a          801 c          911 g         1150 t
ORIGIN      
        1 ggctgggtgt cgtactactg catcattaaa tattacagat ggtattaatg ttggggagat
       61 tttagcgaat gaaacttcct ttagtaaaag tgtcgtgttt actgggatat cttgtgatac
      121 gagcacggat aaaatagttt ataaaaatat ccaaagtgat tgggttgaag ttgggccttt
      181 tggtaatggc gaaaaattaa aggttaaaat agagtcttta ggtaaaacca gcgacacaat
      241 tgggaaatcc agcaatgcgc aggcagtatt accttatgtg gttaaaatag ccagaggcac
      301 acctgatttt actggagaaa gaaaatctac ctggtttatt tcagataccg tgattgcaaa
      361 tattggcggt gagtcatcgt catccatcga tttttggttg ggtatttgta aggcattgaa
      421 gtttaactgg tgtgtgaatt atctcaccag caaactggcg ggggatacat ttacgcttgg
      481 gttaaatatt tcctattatc ctaaaaatac gacctgtaag cctgaaaaca ccgttataaa
      541 agtagatgat atcgccttgt tccagctcag aaatcaggga aagattgcgg cgaacagtaa
      601 ggaaggaaca attacgttga aatgtgataa tcttttcggc gacaaaaaac aagcatcgcg
      661 gaatatggtt gtatatcttt ctagcagtga cttagttaaa ggaagtaata ctattttgcg
      721 tggtaaaaca gataatggtg tagggtttgt gttggatcta acagaaccac caaaagggac
      781 tgaggctgcc attaaaattt cggccaacgg cgatcagggc gcggcgacat cattatggaa
      841 aacagataaa ccaggagttt cattaaatag caacattatt aatataccag tcatggccag
      901 ttactatgta tatgatgaaa aaaaagttaa atctggcgca ctggaagcaa ccgcattaat
      961 caacgtgaaa tacgattaat aacaagcaag atgagcaagt ggctaataat aagaaataat
     1021 aaggtgtctg catttccccc tctctccgag ggggcttttt ttagcgatga taaaaaatct
     1081 caccgtcgta ggctttaatg attttaccgt cggtgtcgct gatcaacacg tacgcgccac
     1141 ccatataggt ccagtgcgtc ccggcatccg gcgcgggcag gttacgcaac tgccactgct
     1201 taatgttgta ctcatcggta cggtacatct ctggtacggt gtcccctggt ttgtaatgct
     1261 taaagtcagc aatgaaactg cttaactcat acttagaaat tcccgaggta ttggtcgaac
     1321 ccgcggtggc tggtgcggcc caggcggcac tggtaaccag caaaagcgca cccagcaaca
     1381 tcttattttt aatggtcata aactctccac tttggggatc tgtaatatca gtcgcatatc
     1441 tttcctgcca tacaatataa ctgacaagcg atattttgtt agtgatccca tgatttcgta
     1501 aaaagtgtaa atgaaaggat gtagcaaaag catcctcatt gagcgagaag tgagcggaat
     1561 aagctgattt aagaatcatc ctgggctaaa aggatagcga agcccaggtg attgttttat
     1621 ctcataatac ccataaagcc atgcactccc atatacaccc cgactaaacc aattaacaga
     1681 ctggaaaaat agggggcgcg tttagcgaga gtgttaaatc cgctccagcg ttttgcaact
     1741 tgttgaacac tgattgccgc tccaacgcct actgtaacca gcgttagtgc cagaccgatg
     1801 ctgaaactga cgaccagtgt tgcgcccagt gtcagggctt tcaactgaat gcaaatcaac
     1861 agcacggtaa ttgctgccgg gcagggaata aggccacctg ttaagccaaa taacaaaatt
     1921 tgccagttgg tgacctctct accatcaaag cgtcgtttaa tgtcattggc atgggctcgt
     1981 gcatgggcat cctgatactc ttcgttgtcg ccatgttcat gatggtgatg atgttcgtgg
     2041 tcgtggtcgt ggtcgtggtc ttgatgatgt tcgtgatcgt ggtgatgatg ctcatgcata
     2101 ttctccagcc agttgcgttc gccgcgccag gtacgccaga acatccagaa agcggtgcta
     2161 ataatgatca ctgcggaaat caactggagc cacggttctg ctgattgagc agtaaagcgc
     2221 ttgctgatca ccatcccgcc aaaggcaatc aaccagacca ctgcggtatg cgaaatggtt
     2281 gctgccagtc cgagcatcac cgcctgttta atggtgcctt tgatggcgat gataaacgcc
     2341 gccatcatcg tttttgagtg ccctggttcc aggccatgaa gcgcaccaag taagatggcg
     2401 ctggggatga agaaccaggc gtttccttgc tgaagaagag ttgtaaattc ggtcatgata
     2461 ataattctta gtattaattc ggcaatctga ttctactccc ccccagtacc tgatactacc
     2521 ccccagtaga ttaatagtgc tatgattttt catgttcttg ttaaccaggt gttgccatgt
     2581 ctcatacaat ccgtgataaa cagaaactga aagcgcgtgc cagtaagatt cagggccagg
     2641 tcgtggcgct caagaaaatg ctcgacgagc cgcacgaatg cgctgcagtt ttacaacaga
     2701 ttgctgctat ccgtggcgcg gtaaacggtc tgatgcggga agtgattaaa ggtcatctaa
     2761 cggaacatat cgttcaccag ggggatgagc taaaacgtga agaagatctg gatgttgttc
     2821 tgaaggtgct ggattcttat atcaaatgaa agtgcctaat atggccgtag ttgaggcaat
     2881 aaatagaggg acaagtgacg ttaagctgaa ttagagtaaa tggcgtgagg acacacgcca
     2941 ttcgaggtta tagtttgatt aattttcgaa aaattgcttc acagcccagc caccatgacc
     3001 acctggagtt atgaatgaaa caaaaccttt gtcatctacg ctctggattt ctaccgtcat
     3061 tgattcatct aacacgcgac atttattgga tgtaatgaga ctcagcatag cctgttggtc
     3121 attgtcctgg atgtaactga tcatctcgta aaagtcagtt aactttttac agttaaatgt
     3181 accttcttta agtgttgcgc tgtctccgtg acttagtgcg aatgcatttg gcatcattgc
     3241 cacaaagatg acggtaacga gtagtttttt cattttagcc tcttataatt cagaacattc
     3301 actgatgttg taagtgcctt ttctaccatc atcgtttacg agatgtagca cgaagatgat
     3361 gggattggca tcaatgaaag atacagagac gttcccgctt tcggtgctat acatctttgc
     3421 aatctgtttt tcaccaatat atgagagatc ccaactcact ccgttcaagc taaaatgcat
     3481 ctgtttgccg tcaatatcga tggaggattg tgccgtaacg gttttcccat tgctgccaat
     3541 ttgaccctgc tgaagagcat cacatttata ggagcttgca taagtagata gaggtagtag
     3601 cagaagtgca gcaaaaattt tactcttcat attttccctt tgcttggatt tgggttacct
     3661 attttggggt agtcattata aaactattaa ttagtaaaac gaacaagttt tcatttgttt
     3721 agcaatggta ttgtttaggt ttgaaattat tacgtatttt catagcaata ggttggataa
     3781 acagcagttc agggaacaag ttgcactatc tacatacttt tccgctttcc attgcatgtt
     3841 aggtaataga tagcctttgc ccgtaagact aaaaatatta gaaatgactc attcgaatac
     3901 cttctattca ccttaatata ggtttgatta ttgtaatcgt ttaatgtgtc ttatgatttt
     3961 ttcctacttt tcatttgcat aatatagggg gcacagcatg ctcttgaaat
//
LOCUS       DAZFBR010000225         3964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFBR010000225 DAZFBR010000000
VERSION     DAZFBR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.227"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            169..732
                     /locus_tag="ACHJZ2_004886"
     CDS             169..732
                     /locus_tag="ACHJZ2_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO4570826.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            887..3247
                     /locus_tag="ACHJZ2_004887"
     CDS             887..3247
                     /locus_tag="ACHJZ2_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO4570827.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(3409..3585)
                     /locus_tag="ACHJZ2_004888"
     CDS             complement(3409..3585)
                     /locus_tag="ACHJZ2_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570828.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(3723..3964)
                     /locus_tag="ACHJZ2_004889"
                     /pseudo
     CDS             complement(3723..3964)
                     /locus_tag="ACHJZ2_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1018 a          825 c         1008 g         1113 t
ORIGIN      
        1 ctctgaaata ttacctcttt ggagtaatga ggtaatacga aacagtttta tcaggttgtc
       61 tgtgctgctg gttacctcgt ttattaatgc cgcggtacgg cgtttctgta ctctcagtat
      121 ttatgtaatt ccaccttcct gtaactttta taaagtaagg actataaaat gtataatgct
      181 aatcccaatt acgaaatgga cttcatgatt ctgaaagacg tgaatgaaca catggaaggt
      241 ctgtttcaac gtttttctaa actgttacct ttcagaattg attttgccta caggaaagac
      301 acgcccagtt ttggtcattc ctgtaggcac agtatgtgta ttgaaatgca ccgcttactt
      361 agtgaaaccc aaacgatgct ggcgggatat tactgggtaa tggaatatac gtcaaacaag
      421 ggacttcata ttcattttat cggctacctg gatgggcaac gtcacaacaa atcttatcgg
      481 atatccaggc aactgggaga tatctggagg cggatcacgg aaggggaggg ttattttcac
      541 ctgtgcaggg ctaaagacaa atacccggtg cgtatcgatc acgtaattca ctatagtgat
      601 aaatctgcgg tagacgactt acgttacgcc cttagctatc tggccaaaca ggatcagaaa
      661 gaacacggta ttattctgag gcgtagcagg cttcccgaaa aaagtaaccg gggccgacca
      721 cgacataact gaattacagc tgtattaggt attagaatta agtttctgta agttgttggg
      781 aggtgcttga gttaataggc agaagagggc ggggcgcttt gtcatcagtg taggggaccc
      841 gaatttctgc cgtggtgcct cagtggatcg gatacaggta acgaatatgg ttcataaatc
      901 tgacagtgat gaattagctg cattgagggc agaaaatgcc cgtctggtct cattacttga
      961 agctcatggg attgaatggc gacgtaaacc gcagactcct gtgcagcgcg tttctgtatt
     1021 atccaccgac gagaaggttg cattatttcg tcggttgttt cgtgggcgtg atgatgtatg
     1081 ggcacttaga tgggaaagta aaaccagcgg caaatcaggg tactctcctg cctgcgctaa
     1141 cgaatggcag gcgagaatat gcggtaaacc ccggataaaa tgtggggact gcgctcaccg
     1201 tcagttgatt cctgtatccg atctcgtcat ctaccaccat ctggccggta cccatactgt
     1261 cgggatgtat ccgttgctgg aagatgattc ctgttatttt ctggcagttg attttgatga
     1321 agctgaatgg cagaaggatg catccgcatt catgcgatcc tgtgatgagc tgggtgtacc
     1381 tgctgcgctg gaaatatccc gttcacgtca gggggcgcat gtctggatat tttttgcctc
     1441 acgagtttcg gcccgcgaag ctcgccgtct ggggactgct attattagct atacgtgtag
     1501 tcggacccga cagctgcgat tggggtctta tgaccgatta tttcctaatc aggatactat
     1561 gccaaaaggg ggatttggta atctcatagc gttacctctg caaaaaagac cgcgtgaatt
     1621 ggggggaagc gtttttgttg atatgaatct ccagccttat cctgatcagt gggcttttct
     1681 tgtatcggtg accccgatga atgtgcagga tattgaaccg acgatattac gggctacagg
     1741 gagtatccat cctctggatg tgaattttat caacgaagaa gacctgggta cgccgtggga
     1801 agagaaaaaa tcatcaggaa acagactgaa tatttctatt gcagaaccgc tgaaaatcac
     1861 gctggcaaac cagatctatt tcgaaaaagc gcaattacct caggtgctga ttaaccgact
     1921 tattcggctg gcagcatttc cgaaccctga gttttataag gctcaggcaa tgcgtatgtc
     1981 agtctggaat aagccccgtg ttataggttg tgcggagaat tacccgcaac acattgcgtt
     2041 gccccgggga tgtctggaca gcgtattatc tttccttagg gacaacaata ttgctgcaga
     2101 attaatcgat aaacgatttg ccgggacgga atgtaatgcc gtttttatgg gaaacctcag
     2161 agcggagcag gaagaggccg tttcggcatt actccgttat gacactggtg tgctttgtgc
     2221 gccaacggct tttggtaaga cagttaccgc agcggcagtg attgccaagc ggaaagtgaa
     2281 tacactgata ctggtacacc ggactgaatt gctgaagcag tggcaggagc gtctcgcggt
     2341 gtttctgcag gccggtgaca gtattggtat tatcggggga ggtaaacata aaccctgtgg
     2401 caatattgat attgcggtgg tgcagtccat atccagacaa ggagaagttg aacctctggt
     2461 caggaattat gggcaaatca ttgtggatga gtgccatcat attggcgcgg tttcattttc
     2521 tgcgattctg aaggaaacga atgccagata tctgcttggc ctgacggcaa caccaatccg
     2581 acgggatggt ctgcatccca ttatttttat gtactgtggt gccattcgcc atacagcggt
     2641 ccgcccgaag gaaagcccac ataatctgga ggtactgatc cgttcccgtt ttacatctgg
     2701 tcatttacca tcggatgcga gaatccagga tattttcaga gaaattgctc tggatcatga
     2761 cagaacggtg gcgatagctg aagaagccat gaaagctttc gggcaggggc gaaaagttct
     2821 ggtactgact gaacgtacag atcatctgga tgagatagca tcagtgatga attcactgaa
     2881 attgtctccc tttattctcc atggtcgact atcgaaaaaa aagcgtgcga tgctgatatc
     2941 cgggctgaat gctcttcctc ccgattctcc tcgaattttg ttgtcaacag gcagacttat
     3001 tggtgaggga tttgaccacc ctccgctgga tacgctgatt cttgccatgc ctgtgtcatg
     3061 gaaagggaca ttacagcagt atgcagggcg tcttcacaga gagcataccg gtaaaagcga
     3121 tgtcaggatc attgattttg tggataccgc gtatcctgtg ctgctcagaa tgtgggataa
     3181 acgtcagcgg ggttataaag cgatggggta caggattata gctgacggcg atgaatcagt
     3241 gatttaattt taaggagtac tttacccttt ttaaataatt tgagctcccc gggttgttcg
     3301 ggcaacaaag agtcccagta tacaggcttc aggaatatgt tgtagtatca ggcttttcat
     3361 cgctttaaaa tgactaccgg cagtgagaac gtcatcaaaa ataacaatct agggcctgca
     3421 cccttccagt aattgttcat ccagttggta gtttgccata atcgcttctg gtaagggccg
     3481 gtttgttgcg gtatgagagg ctattgttga tacgttttgc acaaccagtt cccagaaatc
     3541 cagccatggt tcgtaggttt gcgcaattcg cagaatttct gtcatacggt catcgtaagc
     3601 ggcatctgta cggcatttgg atggtggtac gggaacaaat gtgtattcct caagattacc
     3661 gatatttgat atgagcatct gagcaacata ctcaatagca gcctttttat agtggtaatc
     3721 aggctacgaa agtacagcgt tttataaatt gtctcaggtg atattcgcag cgttttttga
     3781 cgtggttttg ttcgccttaa ccatcctgat atttgctctg gagaccattt catctccagc
     3841 ttttccagaa caagctttcg caatggtaaa ttttgatcca gtaagcacgg ttttggcctt
     3901 ttcgccattc tgttggctcg gttattagca tcaacagctt tgtaatagcg tctgccccga
     3961 ttac
//
LOCUS       DAZFBR010000226         3912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFBR010000226 DAZFBR010000000
VERSION     DAZFBR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.228"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            117..527
                     /gene="ybcV"
                     /locus_tag="ACHJZ2_004890"
     CDS             117..527
                     /gene="ybcV"
                     /locus_tag="ACHJZ2_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO4570829.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYAFDNSLLFTESIQNTTQIFPR"
     gene            819..1112
                     /gene="bor"
                     /locus_tag="ACHJZ2_004891"
     CDS             819..1112
                     /gene="bor"
                     /locus_tag="ACHJZ2_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFO4570830.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(1203..1385)
                     /gene="rzoD"
                     /locus_tag="ACHJZ2_004892"
     CDS             complement(1203..1385)
                     /gene="rzoD"
                     /locus_tag="ACHJZ2_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO4570831.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1602..2078)
                     /locus_tag="ACHJZ2_004893"
     CDS             complement(1602..2078)
                     /locus_tag="ACHJZ2_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO4570832.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            complement(2065..2370)
                     /locus_tag="ACHJZ2_004894"
     CDS             complement(2065..2370)
                     /locus_tag="ACHJZ2_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4570833.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(2692..3381)
                     /locus_tag="ACHJZ2_004895"
     CDS             complement(2692..3381)
                     /locus_tag="ACHJZ2_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4570834.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNLPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            complement(3378..3518)
                     /locus_tag="ACHJZ2_004896"
     CDS             complement(3378..3518)
                     /locus_tag="ACHJZ2_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4570835.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(3515..3877)
                     /gene="rusA"
                     /locus_tag="ACHJZ2_004897"
     CDS             complement(3515..3877)
                     /gene="rusA"
                     /locus_tag="ACHJZ2_004897"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO4570836.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
BASE COUNT         1057 a          965 c          851 g         1039 t
ORIGIN      
        1 cattcctaat atttgacata aatcatcaac aaaacacaaa gaggtcagac cagattgaaa
       61 cgataaaaac gataatgcaa actacgcgcc ctcgtatcac atggaaggtt ttaccaatgg
      121 ctcaggttgc catttttaaa gaaatattcg atcaagtgcg aaaagattta aactgtgaat
      181 tgttttattc tgaactaaaa cgtcacaatg tctcacatta tatttactat ctagccacag
      241 ataatattca cattgtgtta gaaaacgata acactgtgtt aataaaagga cttaaaaagg
      301 ttgtgaatgt taaattctca agaaatacgc atcttataga aacgtcctat gataggttga
      361 aatcaagaga aatcacattt cagcaataca gggaaaatct tgctaaagca ggagttttcc
      421 gatgggttac aaatatccac gaacataaaa gatattacta tgcctttgat aattcattac
      481 tatttactga gagcattcag aacactacac aaatctttcc acgctaaatc atcacgtccg
      541 gtttcttccg tgtcagcacc ggggcgttgg cataatgcaa tacgtgtacg cgctaaaccc
      601 tgtgtgcatc gttattaatt attcccggac actcccgcag aggagtttcc ctgtcagggc
      661 tgcggacata gttaatccgg gaatacagtg acgatccttc gcatctgaca tacattaata
      721 aatattaaca atatgagatt tcaactcatt gtttagggtt tgtttaattt tctacacata
      781 cgattctgcg aactttaaaa agcatcggga ataacaccat gaaaaaaatg ctactcgcta
      841 ctgcgctggc cctgcttatt acaggatgtg cccaacagac gtttactgtt caaaacaaac
      901 cggcagcagt aacaccaaag gaaaccatca cccaccattt cttcgtctct ggaattgggc
      961 agaagaaaac tgtcgatgca gctaaaattt gtggcggcgc agaaaatgtt gttaaaacag
     1021 aaacccagca aacattcgta aatggattgc tcggttttat tactttaggc atttatactc
     1081 cgctggaagc gcgtgtgtat tgctcacaat aattgcatga gctgcccatc gatatgggca
     1141 gctctatctg cactgctcat taatatactt ctgggttcct tccagttgtt tttgcatagt
     1201 gatcagcctc tctctgaggg tgaaataatc ccgttcagcg gtgtctgcca gtcgggggga
     1261 ggctgcatta tccacgccgg aggcggtggt ggcttcacgc actgactgac agactgcttt
     1321 gatgtgcaac cgacgacgac cagcggcaac atcatcatgc agagcatcat tttcagcttt
     1381 cgcatcagct aactccttcg tgtattttgc atcgagcgca gcaacatcac gctgacgcat
     1441 ctgcatgtca gtaattgcca cgttcgccag cttcagttct ctggcatttt tgtcgcgctg
     1501 ggctttgtag gtaatggcgt tatcacggta atgattaaca gcccatgaca ggcagacgat
     1561 gatgcagatg agcagagcgg agataatcgc ggttattctg ctcatacctc actctctctg
     1621 accgttccgc cagcttcttt gaattttgca atcaggctgt cagccttatg ctcgaactga
     1681 ccataaccag cgcccggcag tgaagcccag atattactgc aacggtcgat tgcctgacgg
     1741 atatcaccgc gatcaatcat aggtaaagca ccacgctctt taatctgctg caatgccact
     1801 gcgtcctggc ttttggggga gaagtctttc aaaccaagct gtttacggta agcatcccac
     1861 cagcgtgaaa gaagctgata acgtccggcg gctgttgatt tgagtttggg gtgtagcgtg
     1921 acaagtttgc gagggtgatc ggagtaatca gtaaacagtt cgccgccaac aataacatca
     1981 taaccgtggt tacgtgtcgg ttgtcgcccg ttatccgttc cttctgacca cgccaacata
     2041 tcgaggaagg ctttacgctg aggattaaga ttttgcattt ttcacccctg tcagtcgttc
     2101 ccagaagtac gtcagtgcaa ccgaacccat cgcaccacta atccccgctg tcgcgagaat
     2161 catgtaaata ctgaatccac tttcgatgct gatcaggcca ccaataacac cggtgaatcc
     2221 tgataccact atttgagcca gagcatttat ccaactccac gttgctttac tctgcttcac
     2281 atctatcagg tagcggacca gaccgcccca acctgcgatg atcagcaaaa cgagccagaa
     2341 cgctccggca aggctctctt tttcgtgcat atgaatagcc aatgtttcgc cgccgacaaa
     2401 aggccgggac gttaaatgtc agaaatcagg ctcacggggt aatttaacga caaagcacgg
     2461 agttgatgct ccccacaagc ctggaataaa aaagccagca tgtagctggc aacagagggc
     2521 taagcaatat caactcaaca gctgaagacc ccctggctgg ggtaggttgg aaggctactc
     2581 accgttcaga aacagaaaag cccaaggctt taaacctcgg gcttgaattt ggattactgc
     2641 cagtgcgtac aacattggca aaatatcaga tttacataaa atatatgatt tttaatccag
     2701 ttttgcaata tcttgctgtg aaaatgtggt cttttgtttt gaacgtgttt tcgttaaaag
     2761 caataaagct tggctatcaa gctgtagaaa aatgtgtttc attgcaaccc agcgttcagt
     2821 aaatgtctca gaccagtttt ttgatgtcac tcccaccagt gatgccagct cctggtattc
     2881 ataggtctta cgccctgcca gctcgttctt cacatcctgt gccgccagcc agatcaactt
     2941 cttcaaacgt tccagtgtct tacctgcaat tttcctggta cccaacagag tcttaaactc
     3001 gctccatgcc cactgcgtta tggtgacctg atgttcccag cgaacacttt cgctgtaact
     3061 ccacagcaac cacgctttct gatgttcttc gagagacaga accgcgcggc gccatgacga
     3121 ggttgagaac tcaacctggc tgaccagtgc aatggatgaa ccttttgcgt acgactgctt
     3181 accggaaatc ggcagattat ccagcgtaat catcctgcca gttacctcat ccagaatgcg
     3241 cggcttcttt cgtttgtatg taccagtatc aaattgtgca tgctccagcc aggcttcaag
     3301 ctgtcctttc gttgctccgc tcaaatcagc ggtagccaca atgagttgct cgcggacata
     3361 ctgtaaatat tgggtattca tgcggcagct cctttcagtg ttttggcgta attcttcagt
     3421 atccggtaat cggtcaaaac agaaccagga aaacgatata agcgcaggcg catccagcgg
     3481 tggcgaagac gttctgccat ataagactca aacatcattc attccccagt tcggtgatgg
     3541 tcagctccag cttcccacct ttggtaacgg gcatcttcac aacacggtaa tcaacgacct
     3601 gagcatcatc cagccagaaa cctgctttgg tgagtgcgtc aaaagcggct ttttgcagat
     3661 tatccaggtc acggcgacgg cgatccggca tgtggcactc aatacggatt ttcactggca
     3721 tagccaggcc gatatccagc atggagcctt tgatgattcg ggcgacgtta tcacggtatg
     3781 cctgcccttc tgcgctgatg tgcgtgcgcc cgcgattatg ccggtagtag cgattattgc
     3841 tcggaggcca gggtaatgtg atgctgtagg tattcacgcc ttaataaccc cctctttcag
     3901 ccagataacc tg
//
LOCUS       DAZFBR010000227         3886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFBR010000227 DAZFBR010000000
VERSION     DAZFBR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.229"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(17..2497)
                     /gene="yehB"
                     /locus_tag="ACHJZ2_004898"
     CDS             complement(17..2497)
                     /gene="yehB"
                     /locus_tag="ACHJZ2_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4570837.1"
                     /translation="MLRMTPLASAIVALLLGIEAHAAEETFDTHFMMGGMKGEQVTNL
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPHETCLTREIVKRLGINSDNFAREN
                     QCLTFEQLVQGGSYSWDIGIFRLDLAVPQAWVEELENGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTDNNPGEWKSNTLYLEHGF
                     SQILGTLRIGDMYTSADIFDSVRFTGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFSISDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMHAPGIKDA
                     YVNGQKYRTTNCNGVVVYDGLTPYRENHLMMDVSQSDSETELRGNRKMTAPYRGAVVL
                     VDFDTDQRKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
     gene            complement(2513..3187)
                     /locus_tag="ACHJZ2_004899"
     CDS             complement(2513..3187)
                     /locus_tag="ACHJZ2_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4570838.1"
                     /translation="MKGLLSLLISSMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVVKVAANSGQQIKIKIMPNKLPSNKES
                     IFYLNVLDIPPNSPEDEGKNALKFAMQNRIKLFYRPAGIAPVNKTTFKKLRVSRSGNN
                     VVIKNDSPNWVTISDVKANNVKVNYETIMIAPQESQNVSVKNNNANGWQLTIIDDHGN
                     YISDKI"
     gene            complement(3275..3817)
                     /locus_tag="ACHJZ2_004900"
     CDS             complement(3275..3817)
                     /locus_tag="ACHJZ2_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4570839.1"
                     /translation="MKHSIIAVAVLSSVFMSAGIFAAEVDTGTLTIKGNIAESPCTFV
                     AGGDSVSINMPTVPTTVFDGKAKYSTYDGAVGVTSSMLKITCPKTIDGVKLSLITNEK
                     ILGNDKAISSSNDTVGYYLYLGDSSDVLDISAPFNIESYKTADGQYAIPFKAKYLKLT
                     DSGVASGTVNSSLVMRVAQD"
BASE COUNT         1090 a          928 c          720 g         1148 t
ORIGIN      
        1 catcatattt cacctctcac cgacagatat aatttttact ttcatcaatc tctttaccga
       61 aggtgattgt gcatgaaagc ccttgttgct tatcaattgc cacattaacc gctggcggta
      121 tttcattggt gcgaataaat atctgactgc cctggccgac aacaccaatg ttatgaccat
      181 gcatatcatt gacttcataa ccaaacgtta atggctgtcc atcggatctt aatgctttta
      241 taaaccaggg cttacgctga tcggtatcaa aatcaaccag aacgaccgcg ccgcgataag
      301 gggcggtcat tttacggttc ccgcgtaatt ctgtttcgct atcgctttgc gacacatcca
      361 tcatcaggtg attttcccga taaggtgtca gtccgtcgta caccaccaca ccattacagt
      421 ttgttgtacg atatttttgc ccattgacat aagcatcttt gattcccggc gcatgcatca
      481 cagcaaacgt ttcggaaaga cggttcgcca gattaacgcc accagaccag gcaaccagtc
      541 cccctgaaac actggctccg acctgtcgat aagtactcga ctgactatag ctgccattca
      601 ctgttgcggc cggtgcggtc caggtcaaat tggccccagc tgtcgtttca tttccctgat
      661 gttgatggct caagttgata ccatagttaa actgatcgcg gttcccaacg gttcccgata
      721 atccggtatt gtttgaagca aaaccctgat catcaaaggt cgttgagtta gacatatata
      781 tttgccgtcg cggcgtcgta acgtcatcgc cccagtcaaa gggaatcgat ataaaaatat
      841 taaagcgttt ctcttcggcg tggttctcgt catacgcctg gcttgccgcg agggtatagc
      901 ttatccgccg ccagttattg gaataactca gttgataatc tttactactg ccgcttcgcc
      961 cccagtaatc acgccataaa gtacttaatg acacagatcc ccagccttct ggcaatgact
     1021 gactcatatt ggcggaaaag ctatttttgc gaccaaaatc attctggtaa taatcggcaa
     1081 tatcatagac atcgttttta tcacgacgat aattatcttt attatttgcc cacacataat
     1141 cgttaaatgt gcggtaatca cgcgacgaat aacgccaggc cgccagacca aaacgtgttg
     1201 atgtttggct caaaaatttg ttataggcaa tttgataact ttgtccgtca aacacatcac
     1261 cattgtcttg cttactgtgc gatttagttg catcgactga aattgcgcca atacgcgtgt
     1321 tccaacctgt tccgagggta aaggcatagt aattattagc aaccatcgtg ccgccataca
     1381 gcgtcaataa attattaaaa ccatactgat aacccgcctg gacaaaatca ctttgcttgc
     1441 tcgcaccttc aatatgacta cggcctgccg caaaatcata ttttgataca ccaggttgca
     1501 gcatattagg tactgccgca taaggcacca gatacgtagt cacagaacca tcggcttcct
     1561 taacactaac atcaagatcc gcccctccgc ccgctaactg caaatcacta atcgaaaacg
     1621 gacctggtgg aacctctttc tgataaacga caaaaccgtt ctgttcaata gttaccaacg
     1681 cgttactctg ggcaatcccc tgtacccgtg gggtaaaatt ttgcttcgag tttggcaaca
     1741 tctgcatatc acgaaataac cgaaccccgg tgaagcgaac ggaatcaaat atatcggctg
     1801 aggtgtacat atcaccaata cgcagcgtac cgagaatttg agaaaatcca tgctccagat
     1861 acagcgtgtt gcttttccac tcaccaggat tattatccgt tttgctaaaa ctggcatctg
     1921 aatgcagctg ccatcccagc aaatttagcc cactgttaaa acgcacataa gtgctcttac
     1981 tattgcccga ggctttatag tcactgtaat actgacttac gtaataagac gtataaaacg
     2041 cattgattcc ccgctcccag ttttctggcg gaacgtaacc attttccagc tcttcaaccc
     2101 aggcttgcgg aacagcgaga tccaaacgaa aaatcccaat atcccaggag tagcttccgc
     2161 cctgaacaag ttgctcaaat gttaaacact gattttcgcg tgcaaaatta tcgctattta
     2221 tacctaaccg ctttacaatt tcacgcgtca ggcaagtttc atgtgggtta tctttgacga
     2281 taatctcata ttttccacgc cattgtttat tgacataaat atcaatgtcg tactgcccag
     2341 gtaaaggctg attatcatca agacgcaaat ttgttacttg ctcacctttc attcctccca
     2401 tcataaaatg ggtgtcaaag gtttcttctg ctgcatgtgc ttcaatgccg agcaataacg
     2461 cgactattgc tgatgcaagc ggggtcattc tcaacatagg cagctcctgc aattaaattt
     2521 tgtcactaat atagttgccg tgatcatcga taatggtcag ttgccagcca ttcgcattat
     2581 tattttttac actaacattc tgactttctt gtggtgcaat catgattgtt tcataattca
     2641 ctttgacgtt attagcctta acatcagaaa tcgttaccca atttggcgag tcatttttta
     2701 tgactacgtt attaccgcta cgactcactc gtaatttttt aaaagtcgtt ttatttaccg
     2761 gagcaatacc agcaggtcgg taaaacaact tgattctgtt ttgcatggca aattttaacg
     2821 cattctttcc ttcatcttct gggctattcg gtggaatatc cagaacatta agataaaaaa
     2881 tactttcctt attactgggt aatttattag gcatgatttt aatttttatt tgttgcccag
     2941 aattggccgc tactttcact actggtggcg ttaacatgaa aggaacctga atcttttctg
     3001 gcggcagaga cgtgtcgcca tcatcaatcc acgcctgcat cagcgaagag cggtttccct
     3061 gattcatcag ttgtaccatg acctctttct gctctgccgg gtaaatgata cgggtcccga
     3121 aaataaccat tccggcatgc acggggaata tcatagaaga gatgagtaaa gataataacc
     3181 ctttcatctt aatgcctctt atatttatgc tggccgccat gaatatggcg gccattttaa
     3241 cttactgaaa aaacaagatg attattttaa atatttaatc ctgggcaaca cgcataacca
     3301 atgaagagtt aactgtacca gatgcaacac cactgtcagt caacttcaga tattttgcct
     3361 taaacggtat tgcatattgt ccatcagcag ttttataact ttcaatatta aatggtgcgg
     3421 aaatatccag tacgtcactg ctatctccca aatagagata gtatccaact gtgtcattac
     3481 tactggatat tgccttatca ttaccaagaa ttttctcgtt ggtgatcaac gaaagtttta
     3541 cgccatcaat agtcttcgga caagtaattt tcagcatact gctggttaca ccaaccgcgc
     3601 catcataagt tgaatatttc gctttaccat caaatacagt ggtgggtaca gtcggcatat
     3661 taatactgac tgaatcaccg ccagcgacaa atgtacatgg tgattctgca atattacctt
     3721 taatggttaa agttccggta tctacttcag cagcgaaaat accagcactc ataaatacag
     3781 aagataagac agcgacagca ataattgaat gtttcatgat gcattcctta ataagtttat
     3841 tttgttttgt taagagtctg tatctcttgg aaacaaattt ctcaca
//
LOCUS       DAZFBR010000228         3796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFBR010000228 DAZFBR010000000
VERSION     DAZFBR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.230"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(21..467)
                     /locus_tag="ACHJZ2_004901"
     CDS             complement(21..467)
                     /locus_tag="ACHJZ2_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO4570840.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(464..814)
                     /locus_tag="ACHJZ2_004902"
     CDS             complement(464..814)
                     /locus_tag="ACHJZ2_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO4570841.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(825..1151)
                     /locus_tag="ACHJZ2_004903"
     CDS             complement(825..1151)
                     /locus_tag="ACHJZ2_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4570842.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1148..3408)
                     /locus_tag="ACHJZ2_004904"
                     /pseudo
     CDS             complement(1148..3408)
                     /locus_tag="ACHJZ2_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(3354..3575)
                     /locus_tag="ACHJZ2_004905"
     CDS             complement(3354..3575)
                     /locus_tag="ACHJZ2_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570843.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVM"
     gene            complement(3620..>3796)
                     /locus_tag="ACHJZ2_004906"
     CDS             complement(3620..>3796)
                     /locus_tag="ACHJZ2_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570844.1"
                     /translation="MQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAV
                     AVITGVNYGSASGG"
BASE COUNT          762 a         1270 c          923 g          841 t
ORIGIN      
        1 aggatacaaa tccgcctccg tcatcgtctc agtacctcat caatggcccg gttcatccgc
       61 gcaatcgcca cctgagctgc ctgttcactg cgcacatcaa acgccgggcg cacaaacggg
      121 tgcggtggca tattcacggt ccccatttcc acaaaccgcc agtagaaagc attgcgcggg
      181 ttatccgcct tcatggtgtt atcgctgtta ccggtgtccg gattaacacc acggatatgg
      241 acaccggatt ccatcccgcc atcgcgggag cgccgggaaa ggaccaccac attgcggcgc
      301 agttttcccc tgcgtaccgg tgcccgtgac accacttctt ctttcagcac attcgcaccc
      361 gcacgggttg cctcacgcag cacccggtta ttttctgcac cactcagaag ctgcaaatcg
      421 cggctgatgt cctccagccc cgaaaaatcc agcagggttt cgatcatttt tcacctccca
      481 gccgacagag aatttccaga cgtccgccgg tcgcatccgg cacgggcagc ccgacaacgt
      541 tcaggatccg gtcacgccag ggaccactca gcacatgaag tcgtgacgct gccgtgattt
      601 cccgaccgga ctgaccgcgc acccagatgc ggatttccgc ctgcgccatt tccgcaccag
      661 actgcatccg ctcccggctg ctcctgccac ggatatccgc atgaattttc ccgcatgaca
      721 cccattcttc cgtcatttct ccggcagcat tacgggttaa caccgggttc agaacactta
      781 tcatctgtgt cagacgacct gcagatattg ccattcctcc ctcctcataa caccgtcgga
      841 caacgcaaat cgtaaatcag cacggacaca gaaaacggca gttccccctg cacgaggtct
      901 tcccgctcag caagatccgg attccggtac agcatcccgg tcagtcgcat ggcagccccc
      961 ttcatccggg ttaatgcctc gcccgggatc agctcaccgt cctcacgaat cactttatcc
     1021 cggctgccct gaatgtaggc cagcagcacg gcggtagcct gacgaacctt gtccatcagc
     1081 atgtcatcat ccgcgtcatg gtcaacacgc agatgtgcct tgatctcttc cagtgtcagt
     1141 aatgccgtca ttttccgcct cctgcatccc gtccacgttt tgcagccagg gtccagcctg
     1201 atgaatgagc ttctccgggt ttatcaccgg tcatactgtt gcagtgccac agcgagcccc
     1261 cccacgtcac cgtatcgccg gggtggtagg tttcaccggc tctgaacaca ccgcggtaga
     1321 gcatcaccgg cagggaaaat gttttttccg tacactggcc actgctctgc cggatcacca
     1381 cagagaacaa ccgctcatcc gtcatgctga cgtcgatatc cgccaccccg tcaaccaggc
     1441 attcccatcc ccgcatcccg tgcgtttttt catacgcccg ccagagtcca cccaggtgtg
     1501 tggcatacgt gccccgggga aaggattttt gatcgtcaat agcggggagc acttccagtg
     1561 ccgtggcatc acgcccgtcc tgcggagccg gaagggcatt caccgcctcc agaaccgcct
     1621 gcttcagtac ttccggatcg taatcacgac catcacgcgg agcagggata tggcttacgg
     1681 cctctttcac catctgctca agcatcggac gcacatcatc gggggtgata cttttgccgt
     1741 ccgccggtac cggaatattc gcaaccgcat cattcaccgc ctgctgcagt acatccggat
     1801 cataatcacg accatcacgc ggtaccggaa tggtccccac agcgtcatcc accatcgcct
     1861 gcagaaccgg atgtacctca tccaccgtca catgcttctg taataccgcc gacagggaag
     1921 ccagtttctc ttcaaacgct tgtgcctgcg cggccatctt cccctcaaat gtgcgctgta
     1981 aatccgccag caccgtggag aattcttcgc ccagtgcacg aataatggac agttcccgtt
     2041 ccgtcatttt ctcagtatcc ccctgaacat cgctttcact gcatcatgct ctgtttcact
     2101 gattgcctta ttaccgtcag atgcgccgtc aggcagttgt gctgaaactg ttttcccggc
     2161 cgacgcgaac gggtcctcac gggcatcacg acgggacagc gcctccagac tgtagttctg
     2221 ctgctgaaga tacagtgcat caccgccggc aaggggcggc aggttctcac gtttacgggc
     2281 ctcattgggc gtgagaagcg tatttttcac cgattcaccc agtgttttca tgcgccgttc
     2341 gctgtccatt ctcagcagcg tggtgacgtc aaactccgtg ctctcgtttt cccccgtttc
     2401 cagcgcctca tccagtaaca gctcaatgga ctcaatcagc gtctgcagac actgggaata
     2461 atactgctgc tccagcgcct ccacgttgtc actggaaggc gggtggccaa cgccaatctt
     2521 gtaggccggg acacggaaca ccgaacagac aatttcagcc gtcattttca gttgttccac
     2581 cgtctgcgca tccaccggtg aaaacgtcgt ggggctgtat tttgccccgt tgctcagtat
     2641 ggccgtcttc ccggcatttt cgccggtata cccgctgtcc cagttgctct tcagtttttt
     2701 cgcattttct tccgttatac tgccggggat ctcaatcacc ccggacggcc tgccgccatt
     2761 tctgaaaaaa gacgttgaat ttgcctgaat atgatgcccc tgcgtggccg ccagcccggc
     2821 agcatacacc ggcggcagcc ccacaagcgg atgaaaaaaa cagttaaacc ggtcgtggat
     2881 cacttccctg gcaggcaccg tcaccgcctc cgtgatcccg cagttccggt ccggcgtgat
     2941 gcggtagaac acctcgccgt catccgccac cagaggttca acccggctcc agtccagaat
     3001 acgcagttct ttgatctgcc cccgggagtt acggattttc agcaccaccg tattgccgtg
     3061 acgcagtttg gcgttcagcc acagttcaaa aaactggata cgattctgct gtgcattggg
     3121 acgacgacag agacgggcaa tatccccctg ccgtttttca cggcggatcc cctgtgtatc
     3181 ggtctgcatc aggcgcagtc gcattttggc gatatcctgg gatatcagcg aaatgcaaga
     3241 aaacaccgca tgaaaggaga ggacactttc cggatcggct ttcacgccct gctgccaggc
     3301 gccggcaaaa ggctcagcca ccgcctgaaa caggctggtc cagcccacct cttttacatc
     3361 acgtcctgat ttctggtttt ttcgggttcg ccgcaaaagg ttccacattc gccatgctcc
     3421 gcatcacgtt tctttttctg acctgccgga cgtcgcactg tgatgtactc cgccttcccc
     3481 aggcgaacca gcacctccgc acacggctgt gccacatcac ggatatcccc ggcccgggca
     3541 tcatgcgtgc cctgcagata tcggatcttt gccataacct gttacgggag gcgcacgcct
     3601 cccgtcctcc tcatcagact cagccgccgg acgcactgcc gtagttcact ccggtgatca
     3661 ccgccaccgc cgcggtacgg cgacgacgcc agttgatcca gcgctccgca cggatggcca
     3721 cgctgcctgt ctggaacatg gaaaccagct ccaccgggga cggcgtggta ctgtcgccgg
     3781 tcggctcaga ctgcat
//
LOCUS       DAZFBR010000229         3759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFBR010000229 DAZFBR010000000
VERSION     DAZFBR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.231"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(189..1346)
                     /gene="intS"
                     /locus_tag="ACHJZ2_004907"
     CDS             complement(189..1346)
                     /gene="intS"
                     /locus_tag="ACHJZ2_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO4570845.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(1508..1582)
                     /locus_tag="ACHJZ2_004908"
     tRNA            complement(1508..1582)
                     /locus_tag="ACHJZ2_004908"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1547..1549),aa:Arg,seq:cct)
     gene            complement(1658..2590)
                     /gene="yfdC"
                     /locus_tag="ACHJZ2_004909"
     CDS             complement(1658..2590)
                     /gene="yfdC"
                     /locus_tag="ACHJZ2_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO4570846.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2884..3639
                     /gene="mlaA"
                     /locus_tag="ACHJZ2_004910"
     CDS             2884..3639
                     /gene="mlaA"
                     /locus_tag="ACHJZ2_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO4570847.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          913 a          902 c          882 g         1062 t
ORIGIN      
        1 gcaatcaatt atagcaatta aagtatgata agcatttatg attggtttta atgaaatgca
       61 tatgtagtta acatataata attgcatttt gttattgttg atggtttctt ggtgtaagaa
      121 atcctgagaa gtgaaaagct ttgctatatg tgcaaggctt ttctttttta taagaatcgt
      181 tgtaataact actccacctt cccatcaagc cagtccgccc accactgcat catttctctg
      241 cgcttatcga gatactgagc atggttgtaa atcccacgca cagatccgcc gttggcatgt
      301 gctagttgca cttcaatagc gtcagcaggc cattcgtgct cgttcataat cgtgctgaat
      361 tcatgcctga atccgtgacc gctttccaga ccttcatagc cgatttgttt gatcacaagc
      421 agtacagcgt tctcacagat tgacttcttc ttatcgttgc gcccggcaaa aacaaactct
      481 gatactggtt tagtgatgga gcttagcgta gtgagaagtt caacaacctg gtccgacatc
      541 ggaaccacat gaatcttgcg tcccttcatc acactggcct cgatggtgat aatcctgttt
      601 tcaaaatcga cgttcttcca ttgcatggaa cgaagctctt tcgttcttag tgctgtgtag
      661 cgtaaaactt tggtcgcaat gagagatacg atacttcctg aaaatgttgc aagtgctttg
      721 ttgaatgccg ggatctggtc ggcaggtaaa aacgggaagt tcttcttgcg gtatcccttc
      781 atggcttcag caaggtcagg tgccgggtta tatttagccc ttccggtgac aatagcgtaa
      841 cggaaaacct cgccgcatct tctgcgtgct ttgttggctc gctccattgc cccgcgatct
      901 tcaaatctgc ggattacttc cagcagttgc atcggctcaa tatcctgaat ttcaaggccg
      961 ccgatgatag gtaaaatgtc gtcatcaaac atttttgcaa gttcagtcgc ataggcggct
     1021 gaccagactt gcctcttatg ctcgtaccat tccttgtaaa tcgcactaaa ggaattgttg
     1081 ttagacgaag cctttttcgc ctttaccgga tcgatgccaa ccgagatgtc tttcctcgca
     1141 gtccatgctt tatcccttgc ctcttgcaaa gtcataagcg gatattttcc gacggtcagg
     1201 attttctcct taccgtcaat cttgtagcga agctgccata cctttttccc tgatacaggg
     1261 acataaaggt acaggccatt accatcgagt aggcggtatg gtttttcttt cggctttgct
     1321 gcttcaatct gcttaacggt gagcatgggt aaaaatccgg tgggtaaaat tattttatcc
     1381 actttttacc cgtcatggcg tgcggctgtc aacgatctga cgcgaaccat gacgaactgt
     1441 gaatctacgg aaggcttgat attcagggga ttttgcggac tggtacggat ggaggcgaac
     1501 tgataaatgg tgtcccctgc aggaatcgaa cctgcaatta gcccttagga ggggctcgtt
     1561 atatccattt aactaagagg acaatgcggc atgagtatac ccgctaatgg actgcggggt
     1621 aagtacgctg ccgctcgatt gcttaaaccc tcgccattta tgccgggttt ttatcatttt
     1681 tcttaatgtt ttccgcacgt tctgcttttt ggcgtgcttc tgctttgcgc ttgttgctca
     1741 tgtcgttacg gatctgtgca tgactcatta acgcgaagat aaaggtgccg ccgcagatgt
     1801 tccccgctaa agtaggtagt gcgaagggcc agatgaaatc gctccagtgc agcgtaccgt
     1861 taaacaccag atagaggatt tcaacagaac cgacaacgat gtgagtggtg tcacccaggg
     1921 caataagcca ggtcatcaat ataatcacca caatctttgc cgcacccgct gcaggaaaca
     1981 tccataccat agtggcgatc agccagccgg aaatgatcgc gttggcaaac atctcgctgg
     2041 gggtgttctt catcacatcc atgccgattt tgacaaatgc atcgcgagtt tcttcattga
     2101 agattggcat atattcaaat gcccacgccg caatacctgt cccgagaata ttacccagca
     2161 gcacgacgcc ccataaccgc ataagtaagc caacgttgct cattgtcggt ttttgcatga
     2221 cgggtagtac cgcagtcacg gtgttttcgg taaataattg ctggcgggcc ataatgacga
     2281 taataaaacc aaaggtataa ccgagattct ccagcaagaa gctgcccggc acaccttcca
     2341 gttcgacatg aaatatccct tttgccagta atgaagcgcc catcgacaga cccgccgcaa
     2401 tggctgacca cagtagtgcc attgcgtcgc gttccagctc tttttcacca tcctggcgga
     2461 tatgctcatg aattgccatc gcccgggagg ggagtcgatc ttcatctatt tctatttttt
     2521 tgccacgctc tttttcttcg ctctcaactt caatttcgtc gctgtgttga tcaattttgt
     2581 cgttgtccat ggtctcttct cttgaattag cacgtatagc taaagcgtag cggctttttt
     2641 gctcgcaact ggcgggagtt actttgaaaa tgtagaaaag gccgcgtttg cctttttctg
     2701 tttctataga atcaagtagc ctacagggcg gcgattacca ggctatgatc aaatcagcaa
     2761 atcagggcgt ctggacatca gttgacgtgc tgttacaatc gcccacacct aaacaggcgg
     2821 atacggtatc gttccgtcat ggatggcaaa ctgcataagc cataaaaaaa cagggagaca
     2881 tttatgaagc ttcgcctgtc ggcgcttgct ctgggaacta cgcttctggt ggggtgtgcg
     2941 agttccggta cagatcagca agggcgttct gacccgttag aagggttcaa ccgcaccatg
     3001 tacaacttta acttcaatgt attagacccg tatattgttc gaccggtcgc tgtcgcctgg
     3061 cgtgattatg ttccgcaacc ggcgcgtaac ggtttgagca attttaccgg caaccttgaa
     3121 gaacctgcgg tgatggttaa ctacttcttg cagggcgacc cttatcaggg gatggtccac
     3181 tttacccgct ttttcctgaa caccattttg gggatgggcg gctttattga tgttgcaggg
     3241 atggcgaacc cgaaactgca acggactgaa cctcaccgct tcggtagtac gcttggtcat
     3301 tatggcgtgg gttatggccc gtacgttcaa ttaccgttct acggtagctt cacgctgcgt
     3361 gatgacggtg gtgatatggc ggatggtctt tacccggttc tttcctggct gacctggccg
     3421 atgtctgtgg gtaaatggac gcttgaaggt atcgaaaccc gcgcacagct gctggattcc
     3481 gacggtctgc tgcgtcagtc gtccgatcct tatattatgg tgcgcgaagc gtacttccag
     3541 cgtcatgatt tcatcgctaa tggcggcgaa ctcaaaccgc aggaaaatcc gaacgcgcaa
     3601 gcgattcagg atgatttaaa agatatcgat tctgaataag aaacaaataa aaaaggtgag
     3661 tcgcaaaact cacctttttt gttgttatcc ctcaattacc acaagtaaaa ggagataaac
     3721 cgacaatgac ccggacgtaa ttatgtaaaa atactgatg
//
LOCUS       DAZFBR010000230         3700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFBR010000230 DAZFBR010000000
VERSION     DAZFBR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.232"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(66..482)
                     /locus_tag="ACHJZ2_004911"
                     /pseudo
     CDS             complement(66..482)
                     /locus_tag="ACHJZ2_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(482..667)
                     /locus_tag="ACHJZ2_004912"
     CDS             complement(482..667)
                     /locus_tag="ACHJZ2_004912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570848.1"
                     /translation="MDVRNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
     gene            844..3054
                     /gene="katP"
                     /locus_tag="ACHJZ2_004913"
     CDS             844..3054
                     /gene="katP"
                     /locus_tag="ACHJZ2_004913"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFO4570849.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            3098..3487
                     /locus_tag="ACHJZ2_004914"
     CDS             3098..3487
                     /locus_tag="ACHJZ2_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO4570850.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESIKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            3544..3700
                     /locus_tag="ACHJZ2_004915"
                     /pseudo
     CDS             3544..3700
                     /locus_tag="ACHJZ2_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3600..>3700
                     /locus_tag="ACHJZ2_004915"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1046 a          781 c          951 g          922 t
ORIGIN      
        1 aggagcgaac gtctggttca gcagatttgg cgcaactggc agattgtggt tcgagttcgt
       61 agtcgcacat ctgcctggcg gcagatttcc tgtatcacat ccgcagcaat gcggctcatc
      121 tctgccagta accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac
      181 acaatatgct gccacggaca gtcgggaacc agactcagca gatactgtat ccactgtgcg
      241 ccagccttca ctccgcagtg cgggcaggag cgacttttac accggaagca gacctttttt
      301 atgtggctgc agtccggaga tgaacagcac cactgtgtgt accccatcag cgtggtcccg
      361 caggccatga tttttgtcac agactcaatg actaccggac gcactgaacc ttccggttgt
      421 ttctccagcc agttaagcca gcggtttccc tgctgaaaaa tgtcggcaaa acggggaagc
      481 atcagaaggg cgggggaact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca
      541 tgccgccggc gctgataccg gcaagaatgg tcgcaaactc ccgctccgtg cagcgggcta
      601 tttcaggata cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttctga
      661 catccacaaa gaagggaata ttcaggtctg cgcagcactc aacggcatcg tcagttgcgg
      721 cttggaaccc cttagtattt tttgtctgta gtatctatcc cagcaatagg tatatcctgt
      781 tgcatcaata aagttgactt ttgtatacaa catgcgaatt tcccttaatc cggagctatt
      841 cgtatgataa aaaaaactct tcctgttctg attcttctgg cgctatcggg gagcttttct
      901 accgctgtag ccgctgataa aaaagagact caaaatttct actatccaga aacactggat
      961 ttaactcctc tgagattaca cagccctgaa tcaaatccct ggggggctga ttttgattat
     1021 gccaccagat ttcaacagct ggatatggag gctctgaaaa aagatatcaa agatttgctg
     1081 acaacttccc aggattggtg gcctgcggat tatggtcatt atggtccttt ctttattcgt
     1141 atggcttggc acggtgccgg aacatacagg acatatgatg gccggggagg cgccagtggt
     1201 ggtcagcaac gttttgaacc gctgaacagc tggccggata acgttaatct ggataaagcc
     1261 cgtcgattgc tgtggccagt caagaaaaaa tacggctcca gtatttcctg gggagacctg
     1321 atggtcctga ctggtaatgt tgcccttgaa tccatgggat ttaaaacgct gggatttgct
     1381 ggcggaagag aagatgactg ggagtcggac ctggtatact gggggcctga caacaagcct
     1441 cttgcagata accgggataa aaacgggaaa cttcagaaac ctcttgccgc cacgcagatg
     1501 ggacttattt atgtcaatcc tgaaggcccc ggtggaaaac cagatcctct ggcttccgcg
     1561 aaagatatca gggaagcttt ttcacgtatg gccatggatg atgaggagac tgtggccctg
     1621 atcgcgggag ggcatacatt tggtaaagca catggtgcag cgtctcctga aaaatgtatt
     1681 ggcgcagggc ctgatggtgc acctgtggag gagcagggac tgggatggaa aaataaatgt
     1741 ggtacaggaa acggcaaata taccatcacc agtggcctgg aaggagcctg gtcgacatcg
     1801 ccaacccagt tcacaatgca gtatctgaag aatttatata aatatgaatg ggagctgcac
     1861 aagagtcctg ccggtgctta tcagtggaag cctaaaaaag cggcaaatat agttcaggac
     1921 gcgcatgatc cgtctgtcct gcatccgttg atgatgttta cgacggatat tgctcttaaa
     1981 gttgatcctg aatataagaa aataaccacc cgtttcctga atgatccaaa agcttttgag
     2041 caggcattcg caagagcatg gtttaaactg acccaccggg atatgggacc ggcagcccga
     2101 tatcttggta atgaagttcc tgcagaatca tttatctggc aggatcctct tcctgcggcg
     2161 gattatacaa tgattgatgg taaagacatt aagtcgctga aagagcaggt tatggatttg
     2221 ggtatccctg catctgagct gataaagaca gcctgggctt cagcttccac atttcgtgtg
     2281 actgattatc gtgggggaaa taatggtgcc cgcatcaggt tacagcccga aattaactgg
     2341 gaagttaatg agcctgaaaa actgaagaaa gtactggcat ccctgacctc attacagcgt
     2401 gaatttaaca aaaaacagtc tgacggaaag aaagtgtcgt tggctgattt aattgttctt
     2461 tcgggtaatg ctgcaatcga agatgcggcc agaaaagccg gggtggaact tgagattccc
     2521 tttactccgg gaagaactga cgcctctcag gagcagacgg atgttgcctc attcagtgta
     2581 ctggagccga cagcagatgg attcagaaat tattactcaa aaagcagaag tcatatatcg
     2641 ccggttgaaa gcctcattga taaagccagt cagctggatc tcaccgttcc tgaaatgacg
     2701 gcattactgg gtggtctgcg ggtaatggat attaatacaa ataattcttc gttgggagtg
     2761 tttaccgata cccctggtgt tctggataac aagttttttg ttaatctgct ggatatgtca
     2821 acacgatgga gtaaagcaga taaagaagat acatacaatg gattcgatcg taaaacggga
     2881 gcattaaaat ggaaagcatc ctctgttgat ttaatcttca gttcaaatcc tgaattacgt
     2941 gcggtggcag aagtatatgc ctcggatgat gcgagaaata agtttattca tgattttgtt
     3001 aaatcgtgga ataaagttat gaatagcgat cggtttgatt taaacaataa atgataaaac
     3061 tgatgaggga gggcacctcc ctcatgaagg agaaattatg cagaaaagca ataaagttat
     3121 aatcagctgt ggtatggcat tatatttttt gtgtgggggg attacagtgg cagaagaact
     3181 tgatgaaagc atgcaagttc ttgcaaggaa tatgagtgta cttcagtcat caatggataa
     3241 aaaggagctt cttgaagctc ttgacagaat ggatgatgct gtagatgagt caataaagcg
     3301 tcttcctgag agcatttcac cggctgatga agaagggaag aatgactata taaaagaact
     3361 tcaaaagctt gaaaaagaga ttatgctggc gaaacaaaaa gtcttatcgg gacagttatc
     3421 agatataccc gattcggtaa acaagatgaa taaaatcaga gttgaggggc atgataaatt
     3481 cagataaatc agttggttga ttcctgcggg acgaatacat acgtcaggaa cataacttta
     3541 tggctggaac gtgaaaatcg tgaactgcgc cgcagtaacg atatccttcg ccaggcttcc
     3601 gcttattttg cgaaggcgga gttcgaccgc ctctggaaaa aataatgcca ctgctggata
     3661 agctgcgtga gcagtacggg gtcggaccgg tatgcagtga
//
LOCUS       DAZFBR010000231         3694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFBR010000231 DAZFBR010000000
VERSION     DAZFBR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.233"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..1487)
                     /locus_tag="ACHJZ2_004916"
     CDS             complement(<1..1487)
                     /locus_tag="ACHJZ2_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO4570851.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKYFDRPFS
                     AASGNRKPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMN
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVAT
                     VASAPVIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEA"
     gene            complement(1551..3212)
                     /locus_tag="ACHJZ2_004917"
     CDS             complement(1551..3212)
                     /locus_tag="ACHJZ2_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4570852.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWVHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDESVVGISQGWKLGGAIKTTERKLAEGVLIHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKAYEVFFI"
     gene            complement(3209..>3694)
                     /locus_tag="ACHJZ2_004918"
     CDS             complement(3209..>3694)
                     /locus_tag="ACHJZ2_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4570853.1"
                     /translation="GYSAKTARVKGSQLAKDPEVLAFIARKQCETVEVDEVPVYRQKK
                     SEQEDKPRRREAAAIPQPDENNPEMPPSAVMSPGIEYMEDGLPDPVKAMGRLLVENIN
                     TDPRLALDAAYKLAQFTHHKKGDAGKKSAKGDAAKKAANRFAVPPPPRLVVNNDNEGN
                     G"
BASE COUNT          766 a         1198 c          928 g          802 t
ORIGIN      
        1 gcctctgcat ccgcatccgg gttaccggtt gatgccgtgc ccttcacatc atgggtgatg
       61 gatgccgggg agacatctgc cactgcggct tttttcgggt ccacaaagtc tgtatccaga
      121 cgcgccacca ccgcttccgc cagcgcatta cggaccagtg catcagcagc cggactggaa
      181 aaacggatca attcttccgt cagtaccgca atggccgaca ctttcgcatg actgaaggtg
      241 atggattcaa aatcaaactt cgtcaggggt ctggccttac cctcacccac ccagccggca
      301 gcaccgccgg acacctgggc gtgcacacgg atattgaacg gcacctgacg aagtgcaggg
      361 atcccgccct gaccaaatcg cccgataatg gtctgcggac gcaggtaatc aataaagtcc
      421 tgcgcatatt cctgatattc agacaggctg cctgcccact gtgggtccgt ggtggtccct
      481 gcccccaccg ccgatttcag gacatgatgc agacggctgt catccggata ctgacgacgg
      541 gccacttcca gggcttcaga gcggacacct ttagccgcgg ccagtgattt ggcaaagcgg
      601 gcgaaaccaa tccccttctc cagtttctgc tcaacacgga tcaccggcgc tgaagccacc
      661 gtggccacat tcccgttacc ggcctgtttc accggctgtg ccgtggcggc cttactggtt
      721 tccagttcac gcaggcgctt caggtgcgca tccacctgac ggatttccgc tgcggtgttg
      781 tcgtagtgct cttcctcctc cacatccagc gtgcgccctt cctctgcggc tttgttcatg
      841 atctcctcaa gggaggctgc cagcgccgca cgcttgtttt caaaactttt aatctgttca
      901 ccagtattca ttgctgactt ttccttatga aaagaggtta ttgactgtgc cgaagcgccg
      961 gcagaagatg cgattttcac caccggtttc cggttgccgg acgcggcaga aaacgggcgg
     1021 tcgaaatatt taatggtccg gatggtgcat tccgcattcg cgggcacggt gacggcagac
     1081 acctccatca gctcccagcg cagaaaatgc agtccgcctc cgtccagata agtgtattca
     1141 tggggccgga agcccacaga aagccccctg accagcccgg tcttaatggc agcccaggcc
     1201 tcatccagcc gggctgccat ctgggagggc atccccggct ccggcttcac cagcattgcc
     1261 gtgatttcca gcccttccct gacccgacgc accgtacact ggcctacagg gcgggaatgg
     1321 tcatgctgcc agagaaacgg gatcgtactg ccaaactccg ccccctccgg ctccaggatg
     1381 tcaccatccc gatccggaga aggcgttgac gcaatcccgg tgatcacccg ttcatcctca
     1441 ctgaaggatt tcaccgtcag cagggaacag gcccgtttaa gagtcacatc agcctcctga
     1501 aaataaaaaa accgccgcag cggttcatga tggttacagg gtgagcaggg ttatatgaaa
     1561 aaaacctcat acgctttctt tttcggttcc ggattcaggg acatcaggga caccgcattg
     1621 aagagcgcca tcagcgggtc aatttttccc cgtccgctgg cctgtttggt gataagaatg
     1681 gcgttacctt taggctccac ccgggcatta ccgacacacc aggccatcag gggctgatca
     1741 ccgtgaatca gcaccccttc agccagtttg cgctcggtgg ttttgatggc cccgcccagc
     1801 ttccagccct ggcttatccc caccacactc tcatcgggga tcccggcttc cgccagtgaa
     1861 tccagaatct gccccacacc tgacgggtca ataccgatat gatccagtaa ctcagcctca
     1921 tgaatacgac gcacatactc cgccacttcc gccgtgtcat ccccgacccg acggacaatc
     1981 gtcatgtctc cacaggccac aaaatcctga aaccgggatg cctcactctt ccgtctgacc
     2041 accgcggttt catgcaccca ggcatggccc cagcccagcc attcgcgggt ttccctgtca
     2101 cggccaatca cgtacattcc cagcagatca tccaggcccc cgccgtcaat ccccaccgtc
     2161 accacatcag cgcgctgcag gatatcgtcc aggctgacgc gcctgccctg ctgctcccag
     2221 aaatccgcgc ccgcccagcg gtcagaacgc agggcaagac cgatttccac attggcgtgt
     2281 tttgacatga agccacgaaa tgcttcctca ccagcctccc gggctttacg gtactcccgg
     2341 tacagaaagg cctcatccac cgaataaccg agattcgggt taaccatggc gaggttttcc
     2401 atcagcaggt gagccccgct ttccaccatt tcaggaggat gctcaaaaat caccggcaga
     2461 aagtgcggat catgaatttt gccgtcacgg acatcccggg catactgcag tttctgtctg
     2521 aacaccccgg cgggcggttc attcgactgg gtggtcgtat acaccacaaa cccttccgga
     2581 cgggaggcaa gcccgccgat ggcttcacgt agcatgtctt ccgccttgta ctgcttgcca
     2641 aacagccaca gttcatcaat cagtgtcccc acggacttga taccggacac cgtattcgga
     2701 tcggctgcca ccaccttcag ggtggtgtcc gtcacccgat gggtgatggt ccggatatgt
     2761 gtctgcacct gacagaggtc atccagatca tcgtcccgtc gtaccatatc cctggcaggg
     2821 ttgaaggcgt tagccgccac ctccacggtc ggggccagaa tggtgtagcc cgccgcctgc
     2881 cgccagttca gtaacagcgc cgtcatcatg atccccgcag ccagcgtgga cttactgttt
     2941 ttcttgggga taaggataaa cacttccttg atatggcgaa caccggtctg cgcatcgtag
     3001 gagccaaaca gggccgccac caggtcaaac acccacggtg cacaggactc cccgaatgtc
     3061 gggctacccg gtgcatccac aattcgcagt tgtttaaaaa tcgccagtgc atgtgcagcc
     3121 tggtccggat aaatcggagc cggaataatc gacagcccct ttttcaggcg ctctgcccag
     3181 tccgggcagg ccgtgctcca cacaggtatc atccgttgcc ctcattatca ttattcacca
     3241 ccaggcgggg tggtggtggc accgcaaaac ggttagccgc ttttttcgcc gcgtcacctt
     3301 ttgccgattt tttaccggca tccccttttt tatggtgcgt gaactgcgcc agcttataag
     3361 ccgcatccag cgccagcctg gggtcggtat taatgttctc caccagaaga cgccccatcg
     3421 ctttcaccgg atcgggaaga ccatcctcca tatattcaat accaggagac atcaccgcgg
     3481 acggtggcat ctccggattg ttttcatccg gctgtggtat tgcagccgcc tcacggcgac
     3541 ggggtttatc ctcctgctct gattttttct gccggtaaac aggaacctca tccacctcca
     3601 ccgtctcgca ttgtttacgg gctataaacg caagcacctc cggatctttt gccagctgcg
     3661 agcctttaac cctggcggtc ttcgccgaat aacc
//
LOCUS       DAZFBR010000232         3643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFBR010000232 DAZFBR010000000
VERSION     DAZFBR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.234"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..694
                     /locus_tag="ACHJZ2_004919"
     CDS             <1..694
                     /locus_tag="ACHJZ2_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO4570854.1"
                     /translation="LNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDA
                     VRRTLKEEEKLVNSSDWGYDTQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQI
                     GGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLT
                     LLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMA
                     KRIARLAEQSTD"
     gene            793..1806
                     /gene="ybjS"
                     /locus_tag="ACHJZ2_004920"
     CDS             793..1806
                     /gene="ybjS"
                     /locus_tag="ACHJZ2_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO4570855.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1803..2633)
                     /gene="amiD"
                     /locus_tag="ACHJZ2_004921"
     CDS             complement(1803..2633)
                     /gene="amiD"
                     /locus_tag="ACHJZ2_004921"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO4570856.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2630..2953)
                     /gene="ybjQ"
                     /locus_tag="ACHJZ2_004922"
     CDS             complement(2630..2953)
                     /gene="ybjQ"
                     /locus_tag="ACHJZ2_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO4570857.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3164..>3643)
                     /locus_tag="ACHJZ2_004923"
     CDS             complement(3164..>3643)
                     /locus_tag="ACHJZ2_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO4570858.1"
                     /translation="NCHFFNVDMIRVNFSKCLFHDCSMHGVKIKPWLPVKWTKELIND
                     YLYGCLLSLYSICARDIYNMNAGNNVKVAADAFLEILYSLKNKYCIKLLSAQDRAFIY
                     EFARMIFAYINDKSIEILLLSCFAAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
BASE COUNT          887 a          965 c          892 g          899 t
ORIGIN      
        1 tctcaatgtg attacttccg taccgcccac caccgccagg gcgttgattc aggggctgaa
       61 acacgatctg ctggcggatg acaccgcgct acgtgcgctc atcccacaac ggctgatcgc
      121 tttcgatgac gcggtacgtc gcaccctgaa agaagaagaa aagctggtca actccagcga
      181 ctggggatac gacactcagg cctttgcccg ctggcgacca gagtatggtt attttgccaa
      241 acaggcggga tttaccgtta aaacgtccgc cagccttgcg gctttatggc aggtggtgaa
      301 ccaaatcggc ggtaaagagc gttatttctt tggcaatatt ttgtggcaga cacgggcgtt
      361 gatggaccgt gcgatcggtc ataaattagc gaaaggccgt ccggagcgcg aatatttgca
      421 aactggcgat gcggtggata gctggaaagt gattgtcgtt gaaccggaaa aacaacttac
      481 gttgttattt ggcatgaaag caccggggct gggacgactg tgttttaccc tggaagataa
      541 aggcgactat cgtactatcg atgtccgcgc tttctggcat ccgcacggta tgccggggct
      601 gttttactgg ttattgatga tccccgcgca tctgtttatt tttcgcggaa tggcaaaacg
      661 aatcgccaga ctggcagaac aaagcacaga ttaatcgaca aaacctgcgt tattctttca
      721 tgtttcccat tgcatcaagg gggaaatcac ggaagaatgc gcacgtcatt ttctcaacaa
      781 cagtggattg atatgaaggt actggttacc ggcgccacca gcggcttagg tcgaaacgcg
      841 gtagagtttt tatgccagaa aggcatcagc gtgcgagcga ccggtcgcaa cgaggcaatg
      901 ggtaaattgc tggaaaaaat gggcgcagag tttgttccgg cggatctgac cgagctggtc
      961 tcatcacaag ctaaagtgat gctcgcgggc attgatacgc tgtggcactg ctccagcttt
     1021 acctctccct gggggacaca acaggctttc gatctggcta acgttcgcgc cactcgccgc
     1081 ctaggtgaat gggctgtcgc ctggggtgta cgtaacttta ttcatatctc ttctccctcc
     1141 ctgtacttcg attatcacca ccatcgcgat attaaagaag attttcgccc tcaccgcttc
     1201 gccaacgagt ttgcccgcag caaagcggcc agcgaagaag tgatcaatat gctttcgcag
     1261 gcgaatccac aaacgcgctt tactattctg cgcccacaaa gtctgttcgg accgcacgat
     1321 aaagtcttta tcccccgtct tgcgcatatg atgcaccact acggcagcat tctgttaccg
     1381 catggcggca gtgcgctggt ggatatgacc tactatgaaa atgccgtgca cgccatgtgg
     1441 ctggcaagcc aggaagcctg cgataagcta ccttccgggc gtgtgtacaa catcaccaac
     1501 ggcgagcatc gcacactgcg cagcatcgtg cagaagctga tcgacgagtt gaatattgac
     1561 tgtcgtattc gttccgtccc ttacccgatg ctggatatga tcgcccgcag catggagcgt
     1621 ttaggccgca agtcagcaaa agagccgccg ctgacccact acggcgtctc gaagcttaat
     1681 tttgacttta cgctggatat tacgcgggca caggaagagt taggttatca gccggtcatc
     1741 accctggatg aaggtatcga gaaaactgcc gcctggctgc gcgaccacgg aaaactgccg
     1801 cgctaatcct gcccgtattt ctccagcaac gcttcggcaa tcgcctgagt ttctgcatcc
     1861 gcttcgccgt tatataacgt cgggcggaaa tgcatctgga acgccataat cacgcgccgc
     1921 tgctcgcgcg gtgtcatatc aggtttaacg tcataaccgt agcgcgccaa aagctccagc
     1981 aatgacgcgg tatctaccgg tgtgtgcggc gcgcgcccgg caaggtaaaa gttcacccgc
     2041 tgcgcatccg gccaggcacc aatcccctgc tgcgccagtt gctgccaggg aaataatggc
     2101 cccggatcgt ctttgcgctg cggggcgata tccgcatgtg ccactacgtt ttccggcttg
     2161 atgtgataac gggcaataat atctttcgcc agcggaataa gcgcctgaat ctgtgccggt
     2221 tcaaacgggg caaaatattt cacccccgcc gatttttgcc agccacggtt ttccagctca
     2281 atgccaatag aggtgtcgtt aaggcgcgtt gccccgcgcc aggcgctaat ccccgcatgc
     2341 caggccagtt cttgttccgg caccaattgc cagatgcgcg gtttaccgtt gtatcgcggt
     2401 ggtaccgcag ggaccagata atgcgagctg acctgcttat cggtcagtgt cgccagcgag
     2461 ctatcaaaat catctgcggt gtagtggatc accagcactt taatgcgcgg atacgccgcc
     2521 tgcgcctggc gtcgggtatc aagctgatat ccctctttct cgacaatgcc tttttcgcct
     2581 gcgcaccctg ccagtaacag agcgacagcg atcagccaga agactcttct catcgacgcg
     2641 ttttcaccgc cgtaccgctg acgctaacca tcagcatact gccgttttgc ccgaccgttt
     2701 cgtagtcgat atcaataccg actacggcat cggcccccag cgcccgcgcc tgggagccaa
     2761 gctcctcaaa ggcgatctcc cgcgctttac gtagctcttt ttcgtaggca ccggagcgtc
     2821 cgccaacgat atcgcgaata ccagcaaaga aatcacggaa aatattcgcg cctaaaatcg
     2881 cttcgccagt caccacaccg caatattcaa cgatggtctg gccttccaga gttggagttg
     2941 tggaaaattg catgatcgat ctccttgttt gttaagcctt cgatctctaa agcattatcg
     3001 gactgatatg ccatgttggg aagaaggttt tgatcagttt aggaaatcat ctcattgtgt
     3061 caaaaacagc tacataagcc tgtacaatta tctgtttcgt aaccggatat tggtttcatt
     3121 atggagcacc gcttacctgc cctattatgt tgtgccggca acattaatga gtgggaagag
     3181 tcaacttata ttgtaaatga ttcctaaagt cttccccatc ttgcgcctga ggtatatacc
     3241 gctggattgc tttttggtcc gccgccgcaa aacaagaaag taataagatc tcaatgcttt
     3301 tgtcattaat ataagcgaag atcattcttg caaattcgta tataaacgcc cgatcctgag
     3361 cgcttaatag tttaatacaa tatttatttt taagagaata aagtatttca agaaaagcat
     3421 ccgcagcaac cttcacgtta ttcccggcgt tcatattata tatatctctt gcacagatag
     3481 agtacagaga gagtaaacag ccatacaaat aatcatttat taactctttt gtccatttta
     3541 ctggcaacca tggttttatc ttgacaccat gcatgctaca gtcatgaaaa agacatttgc
     3601 tgaaattaac tcttatcata tcaacattaa aaaaatgaca gtt
//
LOCUS       DAZFBR010000233         3583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFBR010000233 DAZFBR010000000
VERSION     DAZFBR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.235"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..495
                     /locus_tag="ACHJZ2_004924"
     CDS             <1..495
                     /locus_tag="ACHJZ2_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFO4570859.1"
                     /translation="RYITVQRISEVIQRLIQERISVRNMRLVMEALALWSPREKDIIT
                     LVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEM
                     ILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNI
                     LKTI"
     gene            527..1819
                     /gene="sctN"
                     /locus_tag="ACHJZ2_004925"
     CDS             527..1819
                     /gene="sctN"
                     /locus_tag="ACHJZ2_004925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFO4570860.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1809..2267
                     /locus_tag="ACHJZ2_004926"
     CDS             1809..2267
                     /locus_tag="ACHJZ2_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO4570861.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            2245..2478
                     /locus_tag="ACHJZ2_004927"
     CDS             2245..2478
                     /locus_tag="ACHJZ2_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570862.1"
                     /translation="MDEVKKIEHCKILNTDTPDNSASDLDSFLKKNKKNKEMSEIVAG
                     TILQLLRQTQDNQFSTLGKKNGHMKSIDNKSSL"
     gene            2551..3120
                     /locus_tag="ACHJZ2_004928"
     CDS             2551..3120
                     /locus_tag="ACHJZ2_004928"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFO4570863.1"
                     /translation="MTSRDQGINSELSNRTIQFKEKIHNGIHTEYITDQKHSNNKDRE
                     KKYRDGDKINGPQAHSLDITNERRFADNRTMFTQHIEKQRNVNTLNQNDINNSANNAN
                     VRENELTYQFQRWGQNHTVRILESSEGIRLKPSDTLVSDRLHEAQHNDVTAQRWVLTE
                     QDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            3080..>3583
                     /locus_tag="ACHJZ2_004929"
     CDS             3080..>3583
                     /locus_tag="ACHJZ2_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HFO4570864.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYENAPTLDKK
                     SLYWPIHF"
BASE COUNT         1200 a          651 c          810 g          922 t
ORIGIN      
        1 agatatataa ccgtgcagcg catttcagag gtaattcagc gtctgatcca ggagcgaatt
       61 tcagtgcgca atatgcggtt agttatggag gccttagcat tatggagccc acgagagaaa
      121 gatataatca cactcgtcga gcatgtacga ggtgcattag gtcgctacat ttgccataag
      181 ttttcatatt ctggagaaat taaagcaatt gtgatttcgc ctgaaataga ggacagaatc
      241 agagatggtg tcaggcctac cgctggcggt acttttctta atctcgatgc atccgaagct
      301 gagatgattc tggataattt taaactggca ctttctggaa tcaatattcc tattaaagac
      361 attattttac tgggatctgt ggatattcgt cgttttatca aaaaacttat tgaatctagt
      421 tatcgagacc tcgaagtact ttcgtacggg gaactaacgg aaaatgttcc cgttaatatt
      481 ctgaaaacta tttaggataa tgtcttgcga tatggagcat gctggtatga aaaaattaaa
      541 attattaaat aaatacagct acttacatag cataaacggc tctctgattg aagcagaact
      601 tgacgacgtt tctgtgggtg aagtatgcga aatttatgcg agcaggcaag caaatgaacg
      661 tatagccagg gctcaggtcg ttggctttcg caatgggaag acattattaa accttatagg
      721 cagctctgtg gggcttacac gtactgcagt tttgaaacca acgggagagc agttaaccat
      781 acagatcagt gatgcctttc ttggttcggt tttgaatgcc tctggtcaaa ttatggaaag
      841 gtttgtcccg gacactccag gcgatagggg aaatttacgc ctgattgatg aactgccacc
      901 gtcgtatcag gaacgtcgag ttattaatac accacttgag acacggataa gagtcattga
      961 tggcgtactg acatgtggca ttggtcaacg tgttgggatt ttcgcctcgg ctggctgtgg
     1021 caaaacggtt ttaatgcata tgctcgtaaa caatactgaa gctgatgtgt ttgttattgg
     1081 cttgattggc gaacgtggta gggaagttac ggaatgcgcg gaatcgctca aaaaatcagt
     1141 aaatgcagcg aaatgcgtat tggtctatgc cacctctgat ttttcgtcag tagaccggtg
     1201 taatgccgcc ctgatggcga caaccgtggc agagtatttt cgagatcgag gaaagagagt
     1261 tgtacttttt atcgactcaa tgacgcggta tgcacgagca ctacgtgata tgaaactggc
     1321 agcgggtgaa ccacctgcca gacgtggtta tcctgcttct gtttttgaca gtttgcctcg
     1381 cttactggag aggccgggac caacactaaa aggaagtatt accgagtttt ataccgtgtt
     1441 actggaaggg gaagatgaat cagacccact gggtgacgaa attcgctcaa tccttgatgg
     1501 ccatatctat ctaagtcgca aactcgcggg tcaaggtcac tatccggcaa tagatgttct
     1561 aaaaagtgtg agtcgagtat ttggtcaggt cactgatgaa aaacacagag ataatgctgc
     1621 acgggtaagg aagaacctca caactttgga agatctacag gtgtttattg acttagggga
     1681 atatcgcgca gggcaaaatg ctgaaaatga ttttgctatg aatgcccggc ctaaactcac
     1741 aaattggttg aaacagtcag ttaatgaaaa aatgccaatg agtgaaacgc taaaagagct
     1801 tgaacgaatt gttaagtaaa gttaatcgac ttatcagaag aacagcgcag agcctggctg
     1861 catgtgaagc atcattacaa aagttaaatg cagaaaaaga aaagttagcc gaaaaggaac
     1921 gcctgtatga tatgcaactt aaaaacctgc agtcactgtt agatatgaag gagttactcg
     1981 gcgaagttgt atttcgccag gatatatttt actcgttacg taaagttaca gtcattcagc
     2041 agcagatcgc tgagataaat cttgaaaagc aaaaaatagc cgagcggcga aaaatactta
     2101 acaaggaaat agttcaacag caagcacaaa gaaaacactg gtggctaaaa ggagaaaaat
     2161 acgaccgact aaaaaagcgc atcaaaaaac aacttttaaa tcagatgctt tatcaagacg
     2221 agcttgaaca ggaggaaaaa tataatggac gaagtcaaga aaattgaaca ttgtaaaatt
     2281 ttaaacacag acacacccga taattctgct tcggatttag acagtttctt aaagaaaaac
     2341 aaaaaaaata aagaaatgag tgagattgtc gctggaacca ttctccagtt attacgtcag
     2401 actcaggata accaattttc tacgttgggg aaaaagaatg ggcacatgaa gtctattgac
     2461 aacaaatcgt ctctctaggt tgacaggaat ccatctcttt ctttgcaaac aaaaggagcc
     2521 gagcaatttt ctacaaagaa acttctcaat atgacttcac gagatcaggg tattaattct
     2581 gaactatcta atcgaacgat acaatttaag gaaaaaatcc ataatggtat ccatacagaa
     2641 tacatcacag atcaaaaaca cagtaataat aaagatcgtg aaaaaaaata ccgcgatgga
     2701 gacaaaataa atggaccgca ggcccattca ttagacatta ctaacgaaag aaggtttgct
     2761 gataacagaa ccatgtttac tcaacatatc gaaaagcaaa ggaatgttaa tacgctcaat
     2821 cagaatgata taaataattc tgctaataat gccaatgtgc gcgaaaatga attgacatat
     2881 cagtttcagc gttggggaca aaatcatacg gtccggattc tggaaagtag tgaagggata
     2941 cgactaaaac catcagacac acttgtctcc gacagactcc atgaagccca acacaatgat
     3001 gttacagcac aacgttgggt attgactgag caagatgaac gacaaggaca acgacatcag
     3061 cctcatgagg agcaagaaaa tgaaggcaaa ttcgaaaacg atcagaagga tgaaagttaa
     3121 tgttcggtct gaggaaagta aatcgaaaca ctctacgttt gaaaccactt ttcaaaattg
     3181 gaaagaaaat ggcgaagatg ttgcattatt aatgcccgaa tttagtgcta aatggctacc
     3241 gatagcagaa gagagtggca gctggtctgg atgggtgttg cttcgagaaa tatttccctt
     3301 aatttctgcc gagctcgctg ggatggcttt aatgccagaa acggaaaggt taatagggga
     3361 atggctcagt ttatccagtt ccccgttaaa tctaaagtat cctgaactaa aatataatcg
     3421 tttgtgcgta ggtaaagtat ttgatggagt attgagtcca gctcaaccac tgataagaat
     3481 atggacaggg gaattgaatc tttggttaga taaagtcaca gtctgccaat acgaaaacgc
     3541 tccaacttta gacaaaaaat cattatattg gcccattcat ttt
//
LOCUS       DAZFBR010000234         3374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFBR010000234 DAZFBR010000000
VERSION     DAZFBR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.237"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(622..819)
                     /locus_tag="ACHJZ2_004930"
     CDS             complement(622..819)
                     /locus_tag="ACHJZ2_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4570865.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(885..1155)
                     /locus_tag="ACHJZ2_004931"
                     /pseudo
     CDS             complement(885..1155)
                     /locus_tag="ACHJZ2_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1384..1656)
                     /locus_tag="ACHJZ2_004932"
     CDS             complement(1384..1656)
                     /locus_tag="ACHJZ2_004932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570866.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWEAIYRSTSHYGKNVLLLQLA"
     gene            1976..2170
                     /locus_tag="ACHJZ2_004933"
     CDS             1976..2170
                     /locus_tag="ACHJZ2_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570867.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2513..2641
                     /locus_tag="ACHJZ2_004934"
     CDS             2513..2641
                     /locus_tag="ACHJZ2_004934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570868.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2951..3286)
                     /locus_tag="ACHJZ2_004935"
     CDS             complement(2951..3286)
                     /locus_tag="ACHJZ2_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570869.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1001 a          769 c          740 g          864 t
ORIGIN      
        1 ttctggtaga gattcaggat acagtttgta ggagctttct tttttgaaca atgatttgca
       61 ggacagttat gctaaactag cggaatcagt atcacaacaa taaatgactg atcatacctg
      121 aaatttgaat taacaggcat catgaatgtc aatagtctaa gcggtgacta cttatatcgc
      181 ttgcataaat actaaacact gactagaaaa gtattggata accccgtcac taaaagtaac
      241 gcttcggtta tttgctcgga atgtattaac gccaagattg ttagccgtta cgccttatat
      301 cactggcgct gacaaatgtt tcttttctaa tttactgaac cggcgcagac acgcaggaaa
      361 gcacgccggc accggtgtaa tgccagttct aatcaataag aatggaatga cgaatcagtc
      421 aggaagggat aacaacccgc tgctgaacgg aaggtatcag caggaagatg aacgggaata
      481 tcgccagcac caagggcagg gccgggttcc acaataccag cgctggcatt ttgcacattg
      541 ccagcgtacc ggcattacaa cacgtttcat gctgctgccc cccaggattc ctcgattcgt
      601 tgctacatcc actgctctac ttcacttcca ctgctccact tcacttttgt accagcggct
      661 gctgcgcccc agtttgatgg gtttagggaa atggccttca ctgatcaact tgtaaaacca
      721 tttgtcggtc ataccggtga aactggtgat atagttcatg tcgatcaggg aattatcggc
      781 acgcagaaat acgctgtcgt ggcttgttgt agtcaacatg gtcagaatgt cctgtgagat
      841 ttatgaagat tgtgcgaatg cgggaaatct actgagctgt gctttcagaa ctggcctgtt
      901 acgggatacc agggattacc ggcggggtaa cgggcttccg gatcatacac accacgatta
      961 tcgcggacaa aatcactgaa cgcccatatc acctctttaa gtatgtcttc gcagtccggt
     1021 aaatgacgat ccagcgccac atcccgggtg gtacagcttt gatgcgcccg gtgacacaaa
     1081 gcccggattg ttccagacat cctgaatcaa acgccccaga ttaggggcgt cgaaatatgc
     1141 ctctctgacc attatattcc ggcgtatagg tagcaggtct gaagtgacaa tgcgtcacct
     1201 gacgttaaaa gtcacccaag atgaccccag tgggggcaac aagtataaaa ccgttttttg
     1261 attaagtggt cggatatgaa aaggggaagg tttatgtgaa aaatacctgt gttttctttg
     1321 aaaacacaca acagtatcag gttatttatg tttatacagt gcgttccaaa ctgacctgaa
     1381 taattacgca agttgcaaca ataaaacatt tttgccgtag tgtgaggtac tccggtaaat
     1441 tgcttcccag gtgcagacaa tgactaacga agaactacag gcaatcgcat tatccatgct
     1501 tgagcgccag cgcgaaaaag tacaccagca acgagagtct ttgcttaacc catctccatt
     1561 caccaggcca aaacgattgg agcttgaagc attcttggac aaatacccac ggcggcttaa
     1621 atcaggcaaa accagaccac caggctgata gctcacagtt tgttctccga ttcaataaaa
     1681 cggataagaa ccatcagcgc ggtagcgtaa ggcactacag cgcattgctt tacgagtaat
     1741 aacaatggat ttatcaccag aagacgcaaa gtttttacga gaactgcaac accgtatgct
     1801 cagagaagcc attatcgaac agcaaacggg catgagcgcc atagacccat tgcgatacaa
     1861 acgggcctca tctcctttag ttgacgacaa ttccaatgcg gataaaacac gtccaccaca
     1921 ctgattacat ttttcattag cacagtggtg tgatgcagca tacgaggaaa accggatgaa
     1981 tgagaacaaa ataaaaaggc tggagcaact gttacaggca cgccaacggg agtttgccac
     2041 taaatgggga cgtgccgcgc tgccacatga acgcctaacc tattttccac ttgaagacct
     2101 cattaaaaat aatggcaaaa aaatcaggtc tgataagaaa cacttcttag aatgcaatga
     2161 agacaaataa caacaatctg catattgcca ccaaccacag atgtggcatc gttatcagcg
     2221 cacttgattt tctttatatc cactgacgag tttgccggtc attctggtat catgcagata
     2281 tttacggcgt acaagcacac tagcatgaca ttgtaataag gcctgagcct aacgctttaa
     2341 tgtgtgtcat acactcagtg gcgtaatcgt tgacaatatg gtgcatgtaa tcgtaagcgt
     2401 ccagcgaaat ccatctggtc ataatctgaa gcatccgaca aagtggtgtc caccaaataa
     2461 gtgagctgcg cccaatatcc tgagccggaa ctttgacaaa ggccaacgaa aaatgggagc
     2521 ggatgttaca gagttctcgg tacaggggaa aagtggtacc tgtcgccagt tctccgatct
     2581 gtttaaccgg gaaattatct cctacagctt gtcagaaagg tcggtgatgg agcatcgtta
     2641 atacgatgct atacgatgca ttcacagtgc ccggccagag gatgccccgt cgctgcatat
     2701 ggatcagggt tggcaatatc gaattgcagg ctataggcaa agttaaagcc ccatggagta
     2761 gcacaaaata tgccgcacaa aggaaacggt ctgaataacg cagtgatgaa gaacttcttc
     2821 agcacactgc taaaacgcat agtgatcgag cgctgattct ggcgaacaac tgaactaata
     2881 catcagaatc tgcattatgt taaataaata taaaaagatg gtttaaatac cccgttactt
     2941 ttgacttaca ctatacggta tcgcatcgtt taatattcgc accggccaga tttttatttc
     3001 tattagttgt cacaatactg aatgcgtacg accacagtat tctggctcct gtgtggttat
     3061 gctttaattc tgcgttccgg gcagataagc agttgcttgc aggaatcctt cttgtgttaa
     3121 tgtcagttcc ccttttacca gtgctgattt ccacattccg tccaacagag cttatagcct
     3181 ttccctggat tatagcattg tccggctgaa gttctttttg aataataata gaagcactgc
     3241 tggcagatcc agtccgtttt tcataaccca ctgtactgat aaccataatc taatcagtag
     3301 aaattgagtc gaaaataagc actactccat acaggataat tagaggtcag tttgattatt
     3361 cacaattcat catc
//
LOCUS       DAZFBR010000235         3362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFBR010000235 DAZFBR010000000
VERSION     DAZFBR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.238"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            31..390
                     /locus_tag="ACHJZ2_004936"
     CDS             31..390
                     /locus_tag="ACHJZ2_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4570870.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            399..929
                     /locus_tag="ACHJZ2_004937"
     CDS             399..929
                     /locus_tag="ACHJZ2_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO4570871.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            1171..1368
                     /locus_tag="ACHJZ2_004938"
     CDS             1171..1368
                     /locus_tag="ACHJZ2_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4570872.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1519..2577
                     /locus_tag="ACHJZ2_004939"
     CDS             1519..2577
                     /locus_tag="ACHJZ2_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4570873.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            2618..2693
                     /locus_tag="ACHJZ2_004940"
     tRNA            2618..2693
                     /locus_tag="ACHJZ2_004940"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2651..2653,aa:Ile,seq:cat)
     gene            2774..2850
                     /locus_tag="ACHJZ2_004941"
     tRNA            2774..2850
                     /locus_tag="ACHJZ2_004941"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2808..2810,aa:Arg,seq:tct)
BASE COUNT          861 a          731 c          954 g          816 t
ORIGIN      
        1 actggcgtgc tcgggtaaaa gaggtgactg atgctcatag atttggtttt accttacccg
       61 ccgacggtga acacttactg gcgacgccgt ggcagcacat attttgtatc aaaagccggg
      121 gagcgttatc gccgggcagt ggtgcttatt gttcgccagc agcgactgaa attaagcctg
      181 tccggacggc tggcaataaa aattattgca gagccaccgg ataagcgtcg tcgtgacctg
      241 gacaatattc tgaaagcacc gctggatgcg ctgacgcatg cgggagtgtt aatggacgat
      301 gagcagtttg atgaaatcaa tattgtacgt ggtcagccag tatctggtgg acggctgggt
      361 gtgaagattt acaaaattga gagtgagtga gcgtaaatat gatatatccg gaaattacag
      421 gcaaaagcgg cgaacattta cgcctgaaca cgctggaagc agtctggatc caggggaaat
      481 tacggatgtg ggggcggtgg tcgtatatcg gtgggggtaa atccggaaat atgtttaacc
      541 ggttactggt ttcgaaaaag ctgacgaaaa cagcagttaa tgaggtttta cgcagcatga
      601 agaaatccgg gctggaaaaa ccggaacttg aggcattttt tcgggatatg accagaggga
      661 agcagaagag ctggttgtca cattgtacag acacagaggc gttgattatt gatcgcgtta
      721 tcagtgaggt gcttggggaa tatcccgggc taatcaatat tctccggcaa aggtacgaag
      781 gacggggaat gagtaagaga aaaatggcag aatgtttaaa tcgtactcac ccggaatggt
      841 gtttcagcac atgtgagaaa cgtattgcag gttggttagc cgtggctgaa cacatgcttt
      901 atgtacctat gcacgattca tttcgataaa aaaagcttgc ttttttacgc agaaacagct
      961 tgaattcctg taagcttcgc aaagctgtat cgcgaggcga aatgcaagtt ttttcgcaca
     1021 aggaagccac cggaaggtgg tttttttgtg tccgtaatat acagcagcgc aataaattcg
     1081 ctggtggtta ttaataccgt tctttcaggt tgctggcttt ttcgacaaga gttattggtg
     1141 tgtcacgtta accggaaaag ggaaaaagac atgctgaaac agcaggatat gacagaaacc
     1201 gccagagtgg tgtttaatga attaagcgtt accgacccgg cgacagtcgg ggagattgcg
     1261 cagaatactt acctttcacg cgaacgctgc cagttaatac tgacccagct ggttatggcg
     1321 ggtctggcag actatcagtt tggttgttac agacgccttc agtcctgaag gcttttttat
     1381 ttgtggtaaa tgggcggctg gtgggtgtaa ggggcaccca ccagccatct gctcatgcgt
     1441 tgggttcaca agcaaacctc aggcccactg ctttgcgcaa aagcagaatg agcctatcag
     1501 agacaggctt aatgatccat gcttaatact gtaaaaatat ccagttgtga gttaatcaac
     1561 gccgactgcc tggaatttat gcggtcgtta cccgaaaatt ctgttgacct gatagtcacg
     1621 gacccgccgt acttcaaagt gaaacccgag ggctgggata accagtgggc gggtgatgaa
     1681 gattacctga agtggctgga ccagtgtctt gcgcagttct ggcgggtgct gaaacctgcc
     1741 ggaagtcttt acctgttctg tggccatcgt ctggcatctg acaccgaaat catgatgcgt
     1801 gagcggttta acgtgctgaa ccatatcatc tgggcaaagc cgtccggacg ctggaacggg
     1861 tgcaacaagg aaagcctgcg ggcgtatttc cccgccacag agcgcattct gttcgcagag
     1921 cattatcagg ggccgtatcg tccgaaagat gccgggtatg aggcgaaggg taggacactg
     1981 aaacagcatg tgatggcccc gctgattgct tactttcgtg atgcgcgcgc tgtcctgggg
     2041 ataacggcaa aacagattgc agatgccaca ggaaagaaaa acatggtgtc gcactggttc
     2101 agtgccggtc agtggcagct gccgaacgaa agcgattatc tgaaattaca ggcactgttt
     2161 gcccgggtgg cagaagagaa gcatcagcgg ggtgaactgg aaaagcccca ccaccagctg
     2221 gtggatacgt atgcctctct gaaccgacag tatgcggagc tgcagagtga atataagcat
     2281 ctgcggcggt atttcggtgt gacggtgcag gtgccgtaca ccgatgtgtg gacgtataaa
     2341 ccggtgcagt actatccagg gaaacatccg tgcgaaaaac cggcagaaat gttgcagcag
     2401 ataatcagcg caagcagtcg tccgggagac ctggttgcag atttcttcat ggggtcgggg
     2461 tcgacagtga aagcagcgat ggcgctggga cgtcgtgcaa ctggcgttga actggagact
     2521 gaacgttttg agcagacggt gcgggaagta caggatttaa tcattcgtaa cggatgagat
     2581 tgcggagtta atcatgcgtc gttattattc agcaatcggc cctttagctc agcggtgaga
     2641 gcgagcgact cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca ccaaccgcca
     2701 ctagctcatc aggaaagaac gtcaccctgt gcgagattcg gagtccccgg tggcggtcca
     2761 ttatcggtat tctgcgttgt tagctcagcc ggacagagca attgccttct aagcaatcgg
     2821 tcactggttc gaatccagta caacgcacca caccacactt atctgccctg actctctttt
     2881 gcgggctttt tattacagga aagacaccgg acagtgaaat gttaaatgcc tcacaattca
     2941 ggcagttgac tgttgcctga catgctgagc gtttgttaaa aaaatcctgc atgatgaatc
     3001 cccctgagcg gcggggcata atgacagatg tttggttgcg tattgtatag gcaagttgcg
     3061 gattctgtct ggtcattgca gaattcaccg ggaggcaccc ggcatcatgc tgtatacaga
     3121 gattaggcat atatccaggc ttctcatcgc aggagccttt ttacatgcaa aaaaaagccc
     3181 gagtgggttc gggcaacagc atgagatact tgcattgtca tttttatcgt gtggatttta
     3241 accagggttt ataaggctgc gcaactgcgc ggcctttttc gttttgcggg ctgcggttct
     3301 cctcttttga ttctccttgt ggccggaccg tggcccgcaa ctgttgagga aaatcccgga
     3361 aa
//
LOCUS       DAZFBR010000236         3341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFBR010000236 DAZFBR010000000
VERSION     DAZFBR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.239"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..910
                     /locus_tag="ACHJZ2_004942"
     CDS             <1..910
                     /locus_tag="ACHJZ2_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HFO4570874.1"
                     /translation="NRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYQYWRKR
                     RDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSS
                     CQPGKHQYKNARQEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLF
                     ARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYR
                     IKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPH
                     HYNGGLTPEESENRYHFYCKTVASIT"
     gene            919..1071
                     /locus_tag="ACHJZ2_004943"
                     /pseudo
     CDS             919..1071
                     /locus_tag="ACHJZ2_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            1161..1535
                     /locus_tag="ACHJZ2_004944"
     CDS             1161..1535
                     /locus_tag="ACHJZ2_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4570875.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLGRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAK"
     gene            1532..2023
                     /locus_tag="ACHJZ2_004945"
     CDS             1532..2023
                     /locus_tag="ACHJZ2_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4570876.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFTALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGLPT
                     FNW"
     gene            2035..2232
                     /locus_tag="ACHJZ2_004946"
     CDS             2035..2232
                     /locus_tag="ACHJZ2_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4570877.1"
                     /translation="MTSLTPEAALDILIAWLQDNIDSESGIIFDNDEDKTDSAALLPC
                     IEQVREDVRTLRQLQLLQQNR"
     gene            2317..2658
                     /locus_tag="ACHJZ2_004947"
     CDS             2317..2658
                     /locus_tag="ACHJZ2_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570878.1"
                     /translation="MSGIIISRPEVDTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAADIGGKTALDRAMKQDFHCGCWLMEKPETAMKAITRNLDCEIWRDLMQR
                     SGMLSLMDAQG"
     gene            2719..2862
                     /locus_tag="ACHJZ2_004948"
     CDS             2719..2862
                     /locus_tag="ACHJZ2_004948"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570879.1"
                     /translation="MNAFKQLHQSKDEVFERGVINVFRGPNGITKPIPPVNSAVKLSS
                     TTW"
     gene            2856..3275
                     /locus_tag="ACHJZ2_004949"
                     /pseudo
     CDS             2856..3275
                     /locus_tag="ACHJZ2_004949"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT          888 a          864 c          830 g          759 t
ORIGIN      
        1 caaccgcgct cttgatgtcc gactcgctga acggttcacg atagttgcca gactgagtga
       61 cagccactca gttgtcagcc tctgttctgc tctggaaata caccgcagca gttaccagta
      121 ctggcgaaaa cgacgcgata cggttaatcc ggcgcgagtc aggttgtgca gcgaaatacg
      181 ccgggcgtgg aaccaaagta ggggctctgc gggggcgcgc acgctggctg aaatgctgac
      241 ccaaaacggc gtcccgatga gccgttaccg tgccgggcgt ctgatgaaat atctgaacct
      301 gagcagttgt cagcccggaa aacatcagta caaaaatgct cgtcaggaac atacctgcct
      361 gccgaatctg cttgagcgcc agttcgctgt gcccgagcca gatagggtat ggtgcggaga
      421 tattacgtat atctgggcag gaaatcgctg gtgctatctg gcggtcgtca tggatctttt
      481 tgcccgcagg gttatcggct ggagcctgtc agcgaatgcc gatactgccc tgataagcag
      541 tgccctgcgg atggcgtatg aggtgcgtgg tcagccgcgg gacgtcatgt tccatagcga
      601 ccagggaagt caatatacag gactgaaata tcaacaactt ctctggcgtt acaggataaa
      661 gcaaagtgtc agccgacggg gaaactgctg ggataacagt ccaatggaac gcttcttccg
      721 cagtctgaaa acagaatggg tgccaaccga tggctacaca ggcaaagacg tggcccggca
      781 gcaaatcagc agttatattc tgaattatta caatagcgtc aggcctcacc attataacgg
      841 agggctgacg ccggaagagt cagagaacag atatcatttt tactgtaaaa ccgtggccag
      901 tattacttga ccactacact ggaactcatg ctcaccagcg gtgagctgaa tccccgccat
      961 cagcataccg tcacgctgta tgcaaaaggg ctgacctgca aagccgatac cctcggcagt
     1021 cgaggttacg tttatatggc tgtttatccg acgcccgaaa cgaaaaagta aatcatcaga
     1081 ataaccttct gctccggcct ggtgttttca ccacgccact tccccatttc tcatctctgc
     1141 atatcaggaa aaactttagt atgaaaacat tacccgatac acacgtacgg gaggcatcgc
     1201 gctgcccgtc tcccatcacc atctggcaga cactgcttgg ccgtctgctg gaccagcact
     1261 acggcctcac gctgaatgac acaccgttcg ctgatgaacg tgtgattgag cagcatattg
     1321 aggcaggtat ttcactgtgt gatgcggtga actttctcgt ggaaaaatac gtgctggtgc
     1381 gtaccgacca gccgggattc agcgcctgta cccgctctca gttaataaac agcatcgata
     1441 tcctccgggc tcgcagggcg accggcctga tgacccgcga caattacaga acggtaaata
     1501 acattaccct gggtaagcat ccggaggcga aatgatgaaa ctggccctga cactggaagc
     1561 cgacagcgtt aacgtacagg cactgaatat ggggcgaatt gtcgttgaca ttgatggtgt
     1621 taatctcgct gaactgatta acatggtctg cgataacggc tactcccttc gtgttgttga
     1681 tgaatctgac cggacctcag cagactgcac gccaccattt actgccctta ccggcatacg
     1741 ctgcagtacc gcacatatca cggaaacgga caacgcctgg ctgtactcgc tgtcacacca
     1801 gaccagtgac ttcggtgaat cagaatggat tcattttacc ggtaacggct atctgttgcg
     1861 taccgatgcg tggtcgtacc cggttctgcg gcttaaacgc ctgggtctgt caaaaacgtt
     1921 ccgtcgtctg gtcgtcacac tcatccggcg ttatggtgtc agtctcattc atctggatgc
     1981 cagtgccgga tgtctgccag gtttacccac ttttaattgg taactaggaa caacatgaca
     2041 tcattaaccc cggaagcagc actggatatt ctgattgcgt ggctgcagga caatatcgac
     2101 agcgaatccg gaattatctt cgacaacgat gaggataaaa cggattcggc agcattgttg
     2161 ccctgtatcg aacaggtcag ggaagatgtc cgtaccctgc gccaactgca gcttctgcaa
     2221 cagaaccggt gagtctcact caccatactt cattccactg acgccagcct gaacagggct
     2281 ggcgttttcg tttataaacc aaaaggaaaa ccgataatgt caggcatcat aatctcccgt
     2341 ccggaagtgg acaccgggca tacggacgtt atctgctcca ccagtatccg acacgttgtg
     2401 acggtacgga atgcagcact gcaacaaacc gaaacgctta tccggcaact ggcagaaatc
     2461 tcagtcctga cagcagcaga cattggcggc aaaaccgccc tggaccgggc gatgaaacag
     2521 gattttcact gcggctgctg gctgatggag aaacctgaaa ccgcaatgaa agccatcacc
     2581 cgcaacctcg actgcgaaat ctggcgtgac ctgatgcaac gctccgggat gctgagtctg
     2641 atggatgcac agggctagga tacatggtac cgctcactgg agtacgataa tattccggaa
     2701 atcagcgagg cgaatatcat gaatgcattc aaacaactgc accagagtaa ggatgaagtg
     2761 tttgagcgtg gggtgatcaa cgtcttcaga gggccgaacg gaattacaaa accaattccc
     2821 cctgtaaatt cggcagtaaa attatcgtca acaacttggt gagatgggac agatggagat
     2881 ttcatcttat cagtggaatg caggcagatc gcttagctga cctggaaaga atgttgcatc
     2941 tgctcagcgg taaaccgctc cccaacaacc gagggaatat caccattaat ctggatgacc
     3001 acctacagtc cgttcagggt aaagagcgct atgaagacaa gtaaatactt taagaagggc
     3061 ccgcacacat cacattcaga aagccagagc tggttgacag gcttaatgat attattgcca
     3121 gacactacct aggaatgtta ccttcacaaa aatctctaag aattgattca attgtgtatt
     3181 ttagcctcat tccctcatgt aaagtcattt gggggttatt atgggggtca atcatttatc
     3241 caacacgcat taatcattta aaatcaatgc attaataatc atattcgact cctgtgatct
     3301 ccgccaaact tcctcttctg aactctcccg acgtcaacaa a
//
LOCUS       DAZFBR010000237         3303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFBR010000237 DAZFBR010000000
VERSION     DAZFBR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.240"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(201..812)
                     /gene="map"
                     /locus_tag="ACHJZ2_004950"
     CDS             complement(201..812)
                     /gene="map"
                     /locus_tag="ACHJZ2_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HFO4570880.1"
                     /translation="MFSPMTMAGRSLVQATAQTLKPAVTRAAMQAGTGATGMRFMPVQ
                     SNFVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVI
                     SKETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFN
                     TQTKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
     gene            1078..1461
                     /gene="cesF"
                     /locus_tag="ACHJZ2_004951"
     CDS             1078..1461
                     /gene="cesF"
                     /locus_tag="ACHJZ2_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesF"
                     /protein_id="HFO4570881.1"
                     /translation="MNEKFRTDLAHTFGIALEEQTDVLSFHDNDGHEWILECASQSEI
                     LFFYCYLLNSESIQINSILEMNSNRELLGMFFLSLKDDNILLNIAFPADKIDITEFAN
                     LMENGYLLKNEIIRSLSSRPTDFLP"
     gene            complement(1659..2165)
                     /gene="espH"
                     /locus_tag="ACHJZ2_004952"
     CDS             complement(1659..2165)
                     /gene="espH"
                     /locus_tag="ACHJZ2_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HFO4570882.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
     gene            complement(2196..3113)
                     /gene="escQ"
                     /locus_tag="ACHJZ2_004953"
     CDS             complement(2196..3113)
                     /gene="escQ"
                     /locus_tag="ACHJZ2_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFO4570883.1"
                     /translation="MKPLSSQLNMKINDFYLPLLPVIGTGRLYITSKGHACHAYFREV
                     SGHGIRFTLTYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDIYPVIENNNGENRLNVIILETTVQSLQYLINDNWQLVPHS
                     NALFFDGYIVPGWTDYPVTELRVGDSLRLYHVDDSQERRCWIVINTPLATVNLSDNNL
                     SVTDVLAADLLSALSNETVMNRIYCAIGTVHIDIHMLRNVKKDDIINSDGYHLFGGCQ
                     LIRNNTTIAYGSIVKINEDFYFTVSIVCD"
     gene            complement(3076..>3303)
                     /gene="escP"
                     /locus_tag="ACHJZ2_004954"
     CDS             complement(3076..>3303)
                     /gene="escP"
                     /locus_tag="ACHJZ2_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle length
                     regulator EscP"
                     /protein_id="HFO4570884.1"
                     /translation="RYFEEGEHSFSILCGRLRGIVLTIKCSNGIIYLSIKVSPNNRNH
                     VFLYHKKDYVFDKLKEIFPDEAIEFTIEYEN"
BASE COUNT         1030 a          674 c          542 g         1057 t
ORIGIN      
        1 cttaataaaa catttcataa ataaaattgc agcaaacatt catttatatt aaaaaacaca
       61 gccaaagaaa accaattaag caaactataa atcttaatta ataataaagt ttattaagtt
      121 tctttatcgc gttttttctt acatcaacaa aggccatgct atacaccagg aagcttaatt
      181 tatacctgac aaggttatcc ctacaatcgg gtatcctgta catgctctaa aatcgcattc
      241 tgagtaatcg cttttatctc actgactttg aagccgctaa agttgcgttc aaatatttct
      301 tgtgagactt gttctatcat cttcgtctgg gtgttaaaac gtgttattgg atccactctt
      361 ttgccgcact gctcatcaac tttctgatta aagtatttat tggactgcaa gaagtgacca
      421 attgatgatt gtgccccgtt catatcaaga ggttgctgta acgcattttc aacagctcta
      481 aaaatgtgac ctttagtctc cttactaatg accgaattat tactcctgca atagtcatcc
      541 agagttctat ttactgttct ggatatataa gtcgtctgct cctgctggaa ccattgttgg
      601 gtatcgccat tacgagtttg tttggctaca gcctgcagga gttggttagt cagtttgcca
      661 tgattaatca caaagttcga ctgcacgggc atgaacctca tacccgtggc tcccgtaccc
      721 gcttgcattg cagctctggt aaccgcaggt ttaagcgtct gtgcagtcgc ctgaactaac
      781 gatctgcctg ccattgtcat tggactaaac ataccataaa cctttataaa ttatatttcg
      841 acagtaaaaa aattgcttcc cattaaaaaa taactttaaa gaataattat cttgtattta
      901 tatttgaatt atcctccggc cttatctaca ggtagttgca ctacatttat catatgctat
      961 catgaatatg caacactatt cccgtaatct tatatctacg ccttattatt tgtatcagaa
     1021 ctctcggatt aattacgttt gagtatatca acatgaaaag aatggcagga ctacaatatg
     1081 aatgagaaat ttcgcacaga ccttgcacat acgtttggga tagctcttga agagcaaact
     1141 gacgtgctct ctttccacga taacgatggc catgaatgga tactggagtg tgcatctcaa
     1201 tctgaaattc tgttttttta ttgttattta ttgaatagcg aatcaataca gattaattca
     1261 atacttgaaa tgaatagcaa tcgagaactg ctcggtatgt ttttcctgtc attaaaagat
     1321 gacaacattc ttttaaatat tgcatttcct gcagataaaa ttgatattac agagtttgct
     1381 aatttaatgg aaaatggtta tctattaaag aatgagataa ttagatcctt gtcttccaga
     1441 cctacagatt ttttaccttg ataatttctg cgctggatgg gatcatccat tccgtttaac
     1501 atactcttca tcacagggac ctctgatttc tggccgctaa cgtctctgaa gattcagcct
     1561 gtattatcta aaagcaatgt aatatcagtt aaaaagcgga aaaactccag aattataact
     1621 attctggagt taagtcagga agaaaactca aatgctaatc ataatacgct ataagaggaa
     1681 gctcttgttg ctatagccct attatcaaga cgttcggtag actgtacacg acgtaacaaa
     1741 gaagataatc ctctttcagc taagatcccc tccaggggag tacgaggaaa ccaggttgaa
     1801 gaaagtggag ttatatgtct ctcatttaat tttctttcca aagccttagt cacaccgcca
     1861 gaaaacacgc taaatagcgt tgagaaacat ccattttccg gtgttattct ttgtactttg
     1921 tacacataat catcttttgg gccatcttta ataaagtgaa cagcatagtc tttgcctcta
     1981 aaagagacaa tggtggaatc ccctatttct ggatataccc gatgatggcg ttgttgcatt
     2041 cgggctttaa gctgaatttc atctacgata tcttcattgg gtatatgaag ccttctgttg
     2101 aacctgttcc atgacgcatg ttcacttgtc agaaatgtct tgaataccgc tcctgataac
     2161 gacatgatac atctccctat ataacgtatt cctgattaat cacatactat gctaacagta
     2221 aaataaaaat cttcgttgat ttttacaata ctcccgtatg ctatcgtcgt attattgcgt
     2281 attaactgac aaccaccaaa aagatggtat ccatctgaat taataatgtc atctttttta
     2341 acattacgca acatgtgtat atcaatatgg actgtaccga tcgcacaata aatgcgattc
     2401 atgacagtct cattgctaag agcagataac agatccgcag ccagaacatc cgtgacagat
     2461 aaattattat cactcagatt tactgttgct aaaggggtat taataacaat ccagcacctt
     2521 ctctcttgac tatcatcaac atgatagagt ctcagactat caccaactct caactctgta
     2581 actggataat cagtccaccc aggtacgata tagccatcaa aaaaaagagc attagaatgt
     2641 ggaactaatt gccaattatc attaatcaga tattgtaaac tttgtaccgt tgtttcgagt
     2701 ataattacat taagtctgtt ttctccatta ttattttcta ttaccggata aatatcttta
     2761 tttagcatcg taaattgaac atcatcaact gacacatcac cttccggtaa ggcagtctgc
     2821 attacccata agatcatgag ttttatcgta tcttccggta tcagccggct ttcgctatac
     2881 gggaataact cttggcacca ctgaatgaac tgttcttctg atatccagaa acgcccctca
     2941 tagcccgagt atgtcaacgt gaaacgaata ccatgaccgc tgacctcacg aaaatatgca
     3001 tggcaagcat gacctttgga cgtaatatag aggcgaccgg ttccgataac tggcaacaac
     3061 ggcaaataaa aatcgttaat tttcatattc aattgtgaac tcaatggctt catcaggaaa
     3121 gatttctttt aacttatcga aaacatagtc ttttttatga tataaaaaaa catgatttct
     3181 attatttggg cttactttaa tagacagata aatgattccg ttactacatt taattgttaa
     3241 aacaattcct cgtaatcttc cacaaagaat actaaacgag tgctcccctt cttcaaagta
     3301 gcg
//
LOCUS       DAZFBR010000238         3219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFBR010000238 DAZFBR010000000
VERSION     DAZFBR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.241"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            255..713
                     /locus_tag="ACHJZ2_004955"
     CDS             255..713
                     /locus_tag="ACHJZ2_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570885.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            951..1648
                     /locus_tag="ACHJZ2_004956"
     CDS             join(951..1200,1200..1648)
                     /locus_tag="ACHJZ2_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
                     /protein_id="HFO4570886.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            1665..1925
                     /locus_tag="ACHJZ2_004957"
                     /pseudo
     CDS             1665..1925
                     /locus_tag="ACHJZ2_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(2067..3191)
                     /locus_tag="ACHJZ2_004958"
     CDS             complement(2067..3191)
                     /locus_tag="ACHJZ2_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO4570887.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          951 a          703 c          645 g          920 t
ORIGIN      
        1 aattaatgta agaacagatg cccggtagga catatacaca atacatccac aaacacactc
       61 ttttcattaa atcaatcacc tgattaaacc atgaaagtgt gatgtaagtc aaaattactg
      121 atactatcaa ttgataatga ttatctctat cattatcgtg aaattcgaga atgaaaggag
      181 aagtagtcat tataagcctt ttataaatcg tcatcatcat agtaaagtag aacacttttt
      241 atatgagtta ctatatgaaa atatactgga caagaaaacg tattcctgaa ctttcagcac
      301 ttccaccttc attaagaaag aaaaatttta ctgacgcata taatgcagca tcttctcata
      361 tcgaatactg gataggtgct ggcgtctcat ttatctcgat gatgatttta tccagagtat
      421 atgactttct tttacccgcc caagatactt tcccgggtga tatcatcaga agtttatgtg
      481 ttgtgtgtcc atccatatta atctggtttc agttttcggt atatgtcatg aggaaatatt
      541 acagacatat tctggtgcga ggaaaagaaa ccgaaaccat ttcagaaagg ttgatacgtg
      601 aggctgatac acgagaatat gaactatggc gtccggtccg tcgctttttc tccattgttt
      661 tcctcattgt tctattaggt tgcattcatt cactgataac aactataaaa taaaagttat
      721 ggctgttctg atacaggaaa ataaacgttc attctatggt tacctcttcc tgttatatag
      781 aagaatttta aacgacaagt aagggaatat aaatgaaaat aacaaaaaat aaaaatgcaa
      841 ctataaaaaa cagtcatcag tcggctgctt ttcaatgttt accttatcat tttctggtaa
      901 tgctgccaac ttactgattt agtgtatgat ggtgatttta aggtgcttgc gtggcttcca
      961 tttccatcag atgtccttcc tgctccgcta ctgaaggcgt ggtgcgtaac ggcaaaagca
     1021 ctgccggaca tcagcgctat ctctgctctc cttgccgtaa aacatggcaa ctacagttca
     1081 cttacaccgc ctctcagccc ggtaagcacc agaaaatcat tgatatggcc atgaatggcg
     1141 tcggatgtcg cgccagtgca cgcattatgg gcgttggcct caacacggtt ttacgtcact
     1201 taaaaaactc aggccgcagt cggtaacctc gcgcatacaa ccgggcagtg atgtgattgt
     1261 ctgcgctgaa atggacgaac agtggggcta cgtcggtgct aaatcacgtc agcgctggct
     1321 gttttacgcg tatgacagga tacggaggac ggttgtggcg cacgtcttcg gtgaacgcac
     1381 tctggccaca ctggagcgtc ttctgagcct gctgtcggcc tttgaggtcg tggtatggat
     1441 gacggatggc tggccgctgt atgaatcacg cctgaaggga aagctgcacg ttatcagcaa
     1501 gcgttacact cagcgcattg agcgacataa tctgaatctg agacaacatc tggcaaggct
     1561 gggacggaag tcactgtcgt tctcaaaatc ggtggagctg catgacaagg tcatcgggca
     1621 ttatctgaac ataaaacact atcagtaagt tggagtcatt acctgagaat aatacctcct
     1681 gggtaagtga gcagatactt tcagcgcctc cgaaagatgt aaatattcga cttggagtcg
     1741 gttctttaga aggtacaacc gtctcacatg ttcagcgatt gcatcattcg ttaatcgcag
     1801 ctggtttgga aagtaacctc actgtctata ccggtggtca cgattatgcc tggtggcgcg
     1861 gagcaattat tgatgaatta gcaaattata attgcaggaa ggtatcaaat aataactctg
     1921 tgtaaaatat cgttttatcg tcagataact ctttctgcaa tattattatg ggagactatc
     1981 tcatttattt agtgatgtcc ttcatttatg tgatgaagga cattatatta ctcataacat
     2041 ttctcgttga aaatttcagc aatacgctac aattgtttcc cgttgttttt taattttctc
     2101 atcaaaacct ctgcaatctc tgcaggactg ggttgttcag ccatttcagc ggctacctga
     2161 tctgaacaga agcgtagtga atcatcataa agaaggcgat ttaccaaatc actggtcagt
     2221 ccatgcttgt ccggaataat cccacaccca cgcttcgcaa caatttctgc attaacagag
     2281 cgatctgcac cttcgccaaa cacaatctgt ggtattccac tatacagcgc tgtcagggta
     2341 ttacctgcgc caccgtgatg aataaatcca tcagctccat taaggaatac acccataggt
     2401 atccagtcaa ccagacgtac atttgatggt agttttcgta aaccagtacg agcatttatt
     2461 gcaagttgca aaatgatatc agcatcaact tcatttgcag aatccataac ccatgaaatc
     2521 agctccagac catcaaccat tggttttaca gtgccaaggc tgataagtaa acgtttccga
     2581 tcagaatccc tgtcccacca tgtttccttt acagcacctc cgttataagg aatatatctc
     2641 attgagataa ccggttctcc agcattttta agaatgctca tacttggggg agcaacatca
     2701 atccatgcta aatcacatag tggaccgctg actccatgcc gcctgtaagc attgcttaaa
     2761 gaacggttta acatctgaat atgggcagat gtatgcgcga atccaacagc cagcatcact
     2821 gaaggaattc tatatttagc agcaaccaat gggcctgccg gatcaagagg gggatagact
     2881 attaaatctg gcctccattt tcctgcaaaa tcgatgaggt tatctgccat ttcatcgcta
     2941 aaaaaagaga aatgaccaat aatattactt tttttcctca actcttcctg atggcgatat
     3001 ccagcctctg aatctaaacc tggaactgca tcaaaaacaa ccagtccagc ttctgctgct
     3061 ttattcgcga atttgccagc gctactaatt actacatcat gtccaattac acgaaaggcc
     3121 tgagccagag aaatcaatgg gtataacaaa ccgtacagcg gtgggccaat aaaaagaata
     3181 cgttttcgca ttaaaaaagt ccgtcttctg cttacctgt
//
LOCUS       DAZFBR010000239         3213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFBR010000239 DAZFBR010000000
VERSION     DAZFBR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.242"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            166..1848
                     /gene="ydeN"
                     /locus_tag="ACHJZ2_004959"
     CDS             166..1848
                     /gene="ydeN"
                     /locus_tag="ACHJZ2_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4570888.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1900..3057
                     /gene="ydeM"
                     /locus_tag="ACHJZ2_004960"
     CDS             1900..3057
                     /gene="ydeM"
                     /locus_tag="ACHJZ2_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO4570889.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3023..3145)
                     /locus_tag="ACHJZ2_004961"
     CDS             complement(3023..3145)
                     /locus_tag="ACHJZ2_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570890.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          968 a          693 c          650 g          902 t
ORIGIN      
        1 aaattactta gtagaacaaa aagtaatgtc aacgctttat ggactgttat ttcccttttt
       61 ttagctaaat ctgctatctc tttatgtgac taacttcact tacatccact tatttctctt
      121 cgtaaaatta ctttggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta
      181 aagaaaagtg tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt
      241 gctgctcatg cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca
      301 gactttacgc cgactgaata cagtaccaaa ggaaagccaa atattatcgt actgaccatg
      361 gatgatcttg gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg
      421 gaaaatcgtg aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgcg
      481 caaaaatcaa cgccgacgct cctttcatta atggatgaag gcgtacgttt tactaacggc
      541 tatgtggcgc acggtgtttc cggcccctcc cgcgccgcaa taatgaccgg tcgagcgccc
      601 gcccgctttg gtgtctattc caatactgat gctcaggatg gtattccgct aacagaaact
      661 ttcttgcctg aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac
      721 ttgtcaaaaa tcagtaatgt gccggtaccg gaagataaac aaacacgtga ctatcatgac
      781 aacttcacca cattttctgc ggaagaatgg caaccgcaaa accgtggctt tgattacttt
      841 atgggattcc acgctgcagg aacggcatat tacaactccc cttcactgtt caagaatcgt
      901 gaacgtgtcc ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt
      961 gttgatcgtg ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg
     1021 cacctgccaa atgataatcc tgcaccggag caatatcaga agcaatttaa taccggtagt
     1081 caaacagcag ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt
     1141 ctcgaacaac tgaagaaaaa cggacagtat gacaatacaa ttattctctt tacctccgat
     1201 aatggtgcgg ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt
     1261 cagacctatc ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa
     1321 cccggtaatt atgacaagct gatttccgca atggatttct acccgacagc tcttgatgca
     1381 gccgatatca gcattccaaa agaccttaag ctggatggcg tttccttgct gccctggttg
     1441 caagataaga aacaaggcga gccacataaa aatctgacct ggataacctc ttattctcac
     1501 tggtttgatg aggaaaatat tccattctgg gataactacc acaaatttgt ccgtcatcag
     1561 tcagacgatt acccgcataa ccccaacact gaggacttaa gccaattctc ttatacggtg
     1621 agaaataacg attattcgct tgtctataca gtagaaaaca atcagttagg tctctacaaa
     1681 ctgacggatc tacagcaaaa agataacctt gccgccgcca atccgcaggt cgttaaagag
     1741 atgcaaggcg tggtaagaga gtttatcgac agcagccagc caccgcttag cgaggtaaat
     1801 caggagaagt ttaacaatat caagaaagca ctaagcgaag cgaaataact aaaccttcat
     1861 gcggcggatt tttccgccgc cttattgagc gagatagcga tgcacgttac agccaagccc
     1921 tccagttttc aatgtaatct caaatgtgat tactgttttt accttgaaaa agagtcgcag
     1981 tttactcatg aaaaatggat ggatgacagc actctgaaag agttcatcaa acaatatatc
     2041 gcagcgtctg gcaatcaggt ctattttacc tggcaaggcg gtgaacccac tctggctggc
     2101 ctggattttt tccgtaaagt tattcactat caacaacgct atgcaggcca aaaacgtatt
     2161 tttaatgcat tacaaacgaa tggcatttta ttgaataatg aatggtgtgc ctttctcaaa
     2221 gaacatgagt ttctggttgg tatctcgatc gatggacccc aggagctaca tgaccgttac
     2281 agacgcagta attcaggtaa cggtactttt gcaaaagtga tagcagccat cgagcgtctg
     2341 aaatcatatc aagtagagtt taatacgtta accgtcatta ataacgttaa tgtccattac
     2401 cctcttgagg tttatcattt tttaaaatct atcggcagta aacatatgca atttatcgaa
     2461 ttgctcgaaa ccgggacgcc gaatattgat ttcagtggtc atagtgagaa cacattccgt
     2521 atcattgatt tttctgtgcc tcccacggct tatggcaagt ttatgtcaac catttttatg
     2581 caatgggtta aaaacgatgt gggtgaaatt ttcatccgtc agtttgaaag ctttgtcagc
     2641 cgttttttgg ggaatgggca taccagttgt attttccagg agtcctgcaa ggataatctg
     2701 gttgttgaaa gtaatggaga tatttacgaa tgcgatcatt ttgtctatcc acagtacaaa
     2761 cttggaaaca ttaataaatc tgaacttaaa acgatgaaca gtgtacaact gacagcgcaa
     2821 aaaaaacgga tttcagcgaa atgtcagcaa tgtgcatata aacctatctg caatggcggt
     2881 tgccctaagc atcgtattac taaagtaaac gatgagactg tttcctattt ttgcgaaggt
     2941 tataaaatcc ttttttcaac catggtacct tatatgaacg ccatggtgga gttagccaag
     3001 aacagagtac cgctttacca cattatggat gttgcaaaac aaatggagaa taattaataa
     3061 cgctctcttt tagctccatt cttagcctcc ttcttatgac cttaacaagg cacccgacat
     3121 aaattcctgt cgggtgtttc gtcatcatca tcttcccttg cgaaaatctt ggcttacgtg
     3181 gtatgcgtca gggcattagc aatacccttg att
//
LOCUS       DAZFBR010000240         3208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFBR010000240 DAZFBR010000000
VERSION     DAZFBR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.243"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..763
                     /locus_tag="ACHJZ2_004962"
     CDS             <1..763
                     /locus_tag="ACHJZ2_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO4570891.1"
                     /translation="WTAREKNVTLIKQINDLKMCVNEYIGDCTDEELNDREFIASVVD
                     RAIFHFAINSICNPEDNKDATLIERCTFDVETKNGLPSTVQLFYEESKDNEPLANIHL
                     QAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSVYSDFAGRVNINEYNDNYLKAQ
                     FEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLAPDILSKMRDAAPKL
                     IDFAKSFDPNSKEKIKIYTGTSKLIYDLFGVKSEK"
     gene            1125..1229
                     /locus_tag="ACHJZ2_004963"
                     /pseudo
     CDS             1125..1229
                     /locus_tag="ACHJZ2_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            1376..1909
                     /locus_tag="ACHJZ2_004964"
     CDS             1376..1909
                     /locus_tag="ACHJZ2_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570892.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            2106..2279
                     /locus_tag="ACHJZ2_004965"
     CDS             2106..2279
                     /locus_tag="ACHJZ2_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570893.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(2327..2608)
                     /locus_tag="ACHJZ2_004966"
     CDS             complement(2327..2608)
                     /locus_tag="ACHJZ2_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFO4570894.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(2645..2995)
                     /locus_tag="ACHJZ2_004967"
                     /pseudo
     CDS             complement(2645..2995)
                     /locus_tag="ACHJZ2_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(2953..3150)
                     /locus_tag="ACHJZ2_004968"
     CDS             complement(2953..3150)
                     /locus_tag="ACHJZ2_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570895.1"
                     /translation="MAELTKWLQNTITGIETVVDDKSFVCDEIVFKIDVVKNVLTAFK
                     VALVSLEVLMILCRSAMLSQN"
BASE COUNT          893 a          600 c          717 g          998 t
ORIGIN      
        1 atggactgca cgtgaaaaaa atgtgacgtt aattaagcaa ataaacgatc tcaaaatgtg
       61 tgtcaatgag tatattggag attgtactga tgaggaatta aacgatcgtg aatttattgc
      121 atcagttgtt gacagggcta tttttcattt cgcgattaat agtatatgta atcccgagga
      181 taataaagat gcgacactca tagaacgatg tacttttgat gtagaaacca agaatggtct
      241 tccctccacg gttcagctat tttatgagga atctaaagat aatgaacctt tagcgaatat
      301 acatttgcaa gcaataggtt ctggtttctt aacgtttgtt aatgcctgcc aggaacatga
      361 tgacaacagc ttaaaattat ttgcttcgct gttaatttcc ctttcatatt ctagtgtcta
      421 ctcagatttt gcaggaagag tgaatattaa tgaatataat gataactacc tgaaagctca
      481 gtttgaagaa ttatctcaac gtgatatgaa gaagtacctg ggagagatga agcgtctggc
      541 agatggggga gaaatgaatt ttgatggcta tctggataag atgtcacatc tggtgaatga
      601 aggaacgctc gcccctgata ttttaagcaa aatgcgagat gctgcaccga aattaattga
      661 ctttgctaag tcgtttgacc caaactcaaa ggaaaagatt aaaatatata caggcacttc
      721 taaattaatt tatgatttat tcggggttaa atcggagaaa taatatgtga agttcttcga
      781 tggtatgaaa ggtattacat aaaagaaccc aatgtttatt gggttctttt tttctctatc
      841 agtgctatta gcagggagag atatcaccgg agtttaatgt gtgattttta tttatcgtcg
      901 aatctgtatt gtttatcatt gcttttcaca acgctaacat ctaataagat tagatattca
      961 cttcgtgaga gcttcatgcc ttgagaggat ctcaattttc ttttgcaatg agacaggcgc
     1021 ttcctgttgt tatggtatag taccccgtta ttgagcctcc tgaacagtga tgctgaataa
     1081 cataaccaca tgatatatcg ataaaataat ctctacattt gaaaatgcac ggtaattctg
     1141 aaatgcaaaa aatcaaccaa accagcgcaa tgcctgaaaa aactgacgtt cactggagtg
     1201 gtcggtttag cgttgcacca atgctcgata ggatgtaccg tttttgaaaa acaaatagtt
     1261 atatactttg tgggagccta ttgggaaccc ggcgttttca tttcaaggtg taatccatac
     1321 gcggcttaag aatgagatat aatgcgactt ttagtgttcc gcttgagagg ccatgatgct
     1381 taccctggac gagataggtc aatcagtacg taacaatatc cagttgatta ttgatcatgt
     1441 cggcttacct cttgctgttg gtccgctcag tgatgatgat tacaagattc tgtgtggtgg
     1501 ctatggtgag cttgaatggg actatgcgtt aagtacctat ggcaactcca gagaaaagta
     1561 tgagttctgc ataaaacttg ttcagcaagg tcgggttcag ggaataccat caggagcagc
     1621 aatttgtgtt tatggggttg aagaaaacat ctttcgtatc catatgatcg aaaggttttc
     1681 tagagaagat gaatctcacc cattgaaagg gcgcatggtt ttactcactc ttatgagtgc
     1741 ttttatattt tgtaaagctg ttgaatgtaa agttgtccac attgtagagc cagtaccaga
     1801 actggtgcag tattacgagt cttttggttt ccgcatggaa cagtgcggtt atgtgatgtc
     1861 ggcagtcatt gatgagctgc aggatatctt tcttaaattt gctcagtagg tatagacgag
     1921 aagggtctac aaattgtagg atacccgtcc agattacctt aaaggtacat ctatggcagt
     1981 cgttttgtgc ttaaactact aagaaacgat gtcaccaatc gacatgatcg attggcataa
     2041 gttagcgaaa caagctagct ttaaagaaag ggttagagac gcctttactg tctcgggagt
     2101 tttctatgaa agatcaaaaa gcaaccaagc cacaggttaa gttcgacaca atgaaagcat
     2161 tcgcaggtat gggtgctgct gttgaagttc tgatgaaggc tgctcctaat gcgttcactc
     2221 acgctactgt ctctggtaaa gagcagcagg gtaagcttcg tcgtcgcaaa gcagcatgat
     2281 catagctggt gctttttgaa aacccgcctt caggcgggtt ttttctttag tgatgttctt
     2341 tgtccttctg tttgactgtt ctgacctgtt cccactcgat acgtccttct tctcgccttt
     2401 tgtctatgta ttccgcaaga tcttgaatat tgatgcaacg ttttgctttt tgtgatgtgc
     2461 cgatgcgata tgttggaacg ggcaacttac aagcgtttgc ttttgcttct gccgtggctg
     2521 gactcatacc aaagtacttt tggctaactg ctgagagttc aatgtttggg gtattgaatt
     2581 cagccatcag taaaaacaag gtgttcataa ttttctccat caaaaccggc tgcacccggg
     2641 aaaatcataa ttctgtgctg gtggcaggaa ttaatttctg ccagatagcg gaaacatatt
     2701 ttgcctgatg acgggcatca gccagggcgt tgtgccgttc gccatcgaaa ggcatgtcca
     2761 ttttggggtc gaatccgatg gaacgcccaa gcgtaacgat cgtgcgtaca tcgtggtcat
     2821 tccagtacgc ccacgggcag atttgtcctg ctcgctcgta agctccacgt aaaattatgt
     2881 tgtcgaaggt ggccccgtta ccccagactt ttaaatattt cgtattgtct gcgtgccggt
     2941 taatgaaatg gtttagttct gagagagcat cgctgatcga caaagtatca tcaatacttc
     3001 cagcgatacc agcgcgactt taaatgcggt aagtacgttt ttaaccacat cgattttgaa
     3061 tactatttca tcacatacaa acgatttatc gtctactacc gtttcaattc cggtaatcgt
     3121 gttttgtagc catttggtta attcagccat ttttcattac cgccctttcg ggcggtctcc
     3181 tgatgttctg agggtgcagg aatccctc
//
LOCUS       DAZFBR010000241         3191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFBR010000241 DAZFBR010000000
VERSION     DAZFBR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.244"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..289)
                     /locus_tag="ACHJZ2_004969"
     CDS             complement(<1..289)
                     /locus_tag="ACHJZ2_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO4570896.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASD"
     gene            complement(964..1116)
                     /locus_tag="ACHJZ2_004970"
     CDS             complement(964..1116)
                     /locus_tag="ACHJZ2_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570897.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESWLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(1104..1571)
                     /locus_tag="ACHJZ2_004971"
     CDS             complement(1104..1571)
                     /locus_tag="ACHJZ2_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4570898.1"
                     /translation="MSRVTAIISALVICIIVCQSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAKNDALRRKLDNGGRVFVKGKCPVPS
                     SDETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(1568..2065)
                     /gene="rrrD"
                     /locus_tag="ACHJZ2_004972"
     CDS             complement(1568..2065)
                     /gene="rrrD"
                     /locus_tag="ACHJZ2_004972"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO4570899.1"
                     /translation="MSPSLRKAVAAAIGGGAAAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(2065..2280)
                     /locus_tag="ACHJZ2_004973"
     CDS             complement(2065..2280)
                     /locus_tag="ACHJZ2_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4570900.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVVIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            2423..2821
                     /locus_tag="ACHJZ2_004974"
     CDS             2423..2821
                     /locus_tag="ACHJZ2_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570901.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(2902..3060)
                     /locus_tag="ACHJZ2_004975"
     CDS             complement(2902..3060)
                     /locus_tag="ACHJZ2_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO4570902.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
BASE COUNT          866 a          819 c          690 g          816 t
ORIGIN      
        1 catcactggc cggaatgagt tgtgccgttt cctgttcgaa cttaagacgc tcacgttcag
       61 actgatacca ggctttgcgt tcatgtggat ccatttcgcc ctcagcaacc ggcggtggta
      121 accccataaa ttcagtcaga atatcggtca accggtatag cttgagtttg tcatgtccac
      181 cagcgggacg aatgtttttc agtcttgcca cgacagtctg gcggtgcaga ccagataaag
      241 ccgccagttg attaatattc agcaccaggt ttttcaactc atgatccata tttcctccgg
      301 agagctttaa acatgcatcg tgcgaacaac tttaagaaaa cgcgttcgat gtcgaacaaa
      361 aaacactcaa ttcgacatac aaaaaacaaa taaccattaa taatcaataa gatgcaaaga
      421 tgatggtggc cgataaaaat gcaaaaacta gcctttttcc gcgacgctcc cgccccgtgg
      481 caggccaccc caccgggagg agccgtcagc ctgaaagcca tgacgaacgt ctgatacagc
      541 ccttgcatga atggcatcgg gataatccag aaaggaatag catcgaccca caagaatctg
      601 tgtgagtgtc ctgtttcttc cacccccgca caggactggc gagcatgagg gacaaacccg
      661 cgaatcatta gcgcggtaaa aacccggtgt gcatcgtttt tgattattcc cgcacactcg
      721 cgcagaagga gttccccgtc gggctacggt catggttaat gcgggaatac agcgacgata
      781 cagcgcatga tgtgtcaggc ttgaatacct ttatccgtta aaagggatat cagttaagtt
      841 atcccgtgta gggtataagc cattatcaaa gccactctgt agggagtggc ttttgtaatg
      901 gcaataaaaa agccccgcga atgcgaggct aaatccaggt atttttaatg actggctctt
      961 atctcaacgc agccccttac cgcgcgccag atgctcaata tcaagcatca gcaatgagat
     1021 gtttaaccat gattcactcc agaagtgagc accaccctgt ctacagagcc agatgtgaag
     1081 gatgatgagt aaaattatcg ctatcatcga aggcattgcg tcctgatgta ttcctgaagc
     1141 gttctcagtg ctgtttggtc gcggataatt ccgtcccgga taccgagaac gtttcgtcca
     1201 gcaactggag agagttcgac ggtggcatca ttgcccatgc cggaggcgct ggaggtttcg
     1261 tctgaggatg gcacagggca ttttcctttg acgaacaccc gaccaccatt atcaagcttg
     1321 cgccgaagag catcattttt agctttcgca tcagctaact ccttcgtgta tttagcatcg
     1381 agtgcatcag cagcacgctg gcgctgctgc atgtcagtaa tggtggcggt cgcctgcttc
     1441 agctcactga cttttttatc acgctgttct ttataggcga tggcgttatc acggtaatga
     1501 ttaaccgccc atgactggca gacgatgatg cagataacca gagcggagat aatcgcggtt
     1561 actctgctca ttgttgcccc cacaaacaga cttcacgctc aatctcgcgg cgagtcatca
     1621 gccctttcca ttgcttaccg ccagcgtatg tccagcgccg tagctgatca catgcgcctt
     1681 tgatatcacc ctggtttatt ttgcgaagaa gcgtcgatgt tctgaaatta ccagcgccca
     1741 cgttgtaaac gaacgagtaa agagcgccgc gcgttgtttc cggtatatcg actttgatgt
     1801 acgggttaat ttgtctggcg accgtggcaa ggtctttatt caggagggct ttgcattctg
     1861 cttcggtata cgttttaccg ggaatgatgt cttttccggt gtgtccgtga catacagtcc
     1921 atacgccaac gatatcttcg tatggtatgt agctgacacc ttccaggcca tcgtcaccac
     1981 tcggaccagt gatgagcaca gacgctatgg cagcagcccc accaccaata gcagcagcaa
     2041 cagccttgcg taatgatggc gacattattc acctctcgca gccttacgct tatcttcttt
     2101 aatcttgaaa taaaggtttg tcagatacgt cagcaagcca aacaccaagc tacccaatac
     2161 gcctattacc acccactggg atggggagac tttgtccagc aactgcagta gccagtatcc
     2221 cgtccccacc gctgacgtgg tgtatgacac acctgttgtt attttttcca tctggtacat
     2281 accccgtctc ccgttatccg gaagctgaca acaataaaaa gccaccagtt aattcctgat
     2341 ggccctgatg cataaacgtc ataatacctg actgttatga ttgacaataa tgataatgtt
     2401 tatatagaaa ggttcccgat gtgtgttaca tatcatttct ccacggggaa tatccccacg
     2461 ccagcgcaga ctcttttacc cgttctcttc tgcgctggct cttttttatt atgctgctgc
     2521 atttacctct ggcaccatgc tttctatctc aacacaatac gtggtacttc ttgtaaccaa
     2581 tatcataacg attaatcgac atagaatttc tcccgtgtac aggaacagag ttaaaaagcc
     2641 ggaaccggaa tcaaatcaca ggatgaccat ctgccagtgg caggtcataa aaaaaaggcc
     2701 gcgccatgcg cagccagaac tcacaaggaa aatgatagaa ggaaataaca ttagtgatgt
     2761 acgcatggcg cctcccgcta agttctgcaa tgatcaaaca gaactcgcta cgtgccctta
     2821 aaactcgatc atttagcccc tccaaggagg attcaccatg cggttgattt tttaataaac
     2881 agtaaacaaa aaagtcaaga attattcatt ctgttctttc atcatcggcc acagcaatac
     2941 cacaatgccg cagaccagag cgccatcagt cagtaccaac attatcctgc tggtgaaatc
     3001 catcatcacc atcactaaaa gcaggatcac aacagcaagc agacacagtt tataaaacaa
     3061 tgttcaggaa acgcattcag catgcctaag gttctattcc tacgaatagc caacttgcaa
     3121 cttaaaatat tatttatgca gccaattaaa ttctggtcct tacaatatca acctgaagat
     3181 tcttatcttg t
//
LOCUS       DAZFBR010000242         3068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFBR010000242 DAZFBR010000000
VERSION     DAZFBR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.245"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..567
                     /gene="espA"
                     /locus_tag="ACHJZ2_004976"
     CDS             <1..567
                     /gene="espA"
                     /locus_tag="ACHJZ2_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO4570903.1"
                     /translation="NATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQAAILM
                     FSYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAKLPQD
                     VIDYINDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEIQQMS
                     NTLNLLTSARSDVQSLQYRTISAISLGK"
     gene            580..1704
                     /gene="espD"
                     /locus_tag="ACHJZ2_004977"
     CDS             580..1704
                     /gene="espD"
                     /locus_tag="ACHJZ2_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HFO4570904.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            1725..2663
                     /gene="espB"
                     /locus_tag="ACHJZ2_004978"
     CDS             1725..2663
                     /gene="espB"
                     /locus_tag="ACHJZ2_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO4570905.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            2670..>3068
                     /gene="cesD2"
                     /locus_tag="ACHJZ2_004979"
     CDS             2670..>3068
                     /gene="cesD2"
                     /locus_tag="ACHJZ2_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO4570906.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNV"
BASE COUNT          887 a          606 c          766 g          809 t
ORIGIN      
        1 aatgcaacat ccgttgttaa tgtgagtgcg agttcttcga catcgacgat ctatgactta
       61 ggtaatatgt cgaaggatga ggtggttaag ctatttgagg aactcggtgt ttttcaggct
      121 gcgattctca tgttttctta tatgtatcag gcacaaagta atctgtcgat tgcaaagttt
      181 gctgatatga atgaggcatc taaagcgtca accacggcac aaaagatggc taatcttgtg
      241 gatgccaaaa ttgctgatgt tcagagtagc actgataaga atgcgaaagc caaacttcct
      301 caagacgtga ttgactatat aaacgatcca cgtaatgaca taagtgtaac tggtattcgt
      361 gatcttagtg gtgatttaag cgctggtgat ctgcaaacag tgaaggcggc tatttcagct
      421 aaagcgaata acctgacaac ggtagtgaat aatagccagc tcgaaattca gcaaatgtcg
      481 aatacattaa atctcttaac gagtgcacgt tctgatgtgc aatctctaca atatagaact
      541 atttcagcaa tatcccttgg taaataaccg gagataacta tgcttaacgt aaataacgat
      601 accctgtctg taacgtctgg ggttaatacc gcctcgggta cttctggtat tactcaatct
      661 gaaacgggtt tatcgctgga tttacaactg gttaaatcca tgaactcgtc agcaggctgg
      721 acagaaagta gccctttacc gacgccgccg gcaggtcact cattagtgac gccctctgct
      781 gctgaggatg tccttagtaa attgtttggt ggtattagtg gtgaggttac aagtcgcact
      841 gaggaggcag agccacagcg cacaagctat ccctatctct ctcaggtgaa taccgttgac
      901 cctcagcaaa tgatgatgat ggtcactctg ttatccctgg atacttccgc gcagaaagtc
      961 tcgagtctga aaaactctaa cgagatttat atggatgggc aaactaaagc gctggagaat
     1021 aaaacgcagg agtataaaaa acagctcgaa gaacaacaga aagccgaaga gaaatcacaa
     1081 aaaagtaaaa ttgttggcca ggtctttggt tggttgggcg tcgcattaac agccgttgcc
     1141 gctgttttta acccagcact ctgggctgtt gttgccattg gtgcaacagc aatggcactg
     1201 caaacggcag tcgatgtaat gggggaaaat gcccctcagg gattaaagac tgcagcacag
     1261 gtctttggcg gaatatctat ggccgcaagc attctgacag ccggcgttgg cggggtgtct
     1321 tcactgttat ctaaatttgg taatgttgct aacaaaattg gctcaagcgt tgtaaaagtc
     1381 gttgagaagg cggcagaagc gctggttaaa aacgtttttg caaaaatttc gacagtggct
     1441 gagggcgtta cgaacggtat tcgttctgcc gggacaactg cgttgaataa tgaggctgcg
     1501 caactccaaa tgttgtctca gttagctgct ttcgcggtgc aaaacttaac tcgacagagt
     1561 gaaagcttag gtgagagtgc gaagctcgag ctggataaag cggcaagcga gttacaaaat
     1621 caggcgagct atttacaaag tgtttctcaa ctgatgtccg attcagcacg ggtaaatagt
     1681 cgtattgtta gtggccgaat ttaattatta aagagaattt tattatgaat actattgata
     1741 atactcaagt aacgatggtt aattccgctt cggagagtac gaccggcgct tccagtgcag
     1801 ttgccgcatc tgctttatca attgattcat ctctgcttac tgatggtaag gttgatattt
     1861 gtaagctgat gctggaaatt caaaaactcc tcggcaagat ggtgactcta ttgcaggatt
     1921 accaacaaaa acaattggcg caaagctatc agattcagca ggccgttttt gagagccaga
     1981 ataaagctat tgaggaaaaa aaagccgcgg caaccgctgc tttggttggc gggattattt
     2041 catcagcatt ggggatctta ggttcttttg cagcaatgaa caacgcggct aaaggggctg
     2101 gtgagattgc tgaaaaagca agctctgcat cttcaaaggc tgctggtgcg gcttctgagg
     2161 ttgcaaataa agctctggtc aaggctacgg aaagtgttgc tgatgtcgca gaggaggcat
     2221 ccagtgcgat gcagaaagcg atggccacaa caacgaaagc agccagccgt gcatctggcg
     2281 ttgcagatga tgttgcgaaa gcctctgact ttgctgaaga tcttgcagac gccgccgaga
     2341 agacaagcag aatcaataag ttgttgaatt ccgtagataa actgaccaat accacagcat
     2401 ttgttgccgt gaccagtctt gctgaaggta cgaaaacgtt gccaacaaca atatctgagt
     2461 ccgtcaaatc gactcatgag gttaatgaac aacgtgcgaa gtcgctggaa aacttccagc
     2521 aggggaatct ggagctgtat aaacaagacg ttcgcagaac gcaggatgat atcacgactc
     2581 gtctgcgtga tataacgtcc gctgtccgcg atctccttga ggtccagaat cgtatggggc
     2641 aatcgggtcg cttagctggg taattgatca tggtcgatac gtttaatgat gaagtgttta
     2701 attactatct tgaacaaaaa gggtacacaa tacagaagga gtttctttgt ggcagtgcct
     2761 tttttatcgg atggcggatt gagacacctt ttttttcatt agcgtacaga ctggatgaac
     2821 aagaactgat tttgtgctct tttgaagcac gtaaccaaac agggcttaac ggccctgttt
     2881 tatcgctgac tcatttgctc gaagagttgt atcaccattt ttcgggtatt aagaaaatca
     2941 gcgcgatgaa atctaagatt ggttcagatt cagaacgtca aaagcgcgaa gagttgttta
     3001 attacttcat cagaaagggt gccgtgcagc aagaaacaga agacggaatt tggttcgtaa
     3061 tgaacgta
//
LOCUS       DAZFBR010000243         3043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFBR010000243 DAZFBR010000000
VERSION     DAZFBR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.246"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(3..218)
                     /locus_tag="ACHJZ2_004980"
     CDS             complement(3..218)
                     /locus_tag="ACHJZ2_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4570907.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(294..563)
                     /locus_tag="ACHJZ2_004981"
     CDS             complement(294..563)
                     /locus_tag="ACHJZ2_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4570908.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPGGEMMM"
     gene            complement(601..783)
                     /locus_tag="ACHJZ2_004982"
     CDS             complement(601..783)
                     /locus_tag="ACHJZ2_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4570909.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            complement(931..2868)
                     /locus_tag="ACHJZ2_004983"
     CDS             complement(931..2868)
                     /locus_tag="ACHJZ2_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4570910.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVPGTGDGGYPGVVTTEPDYYYVIPLAGQSNGMAYGEGLPLPQTY
                     DRPDPRIKQLARRSTVTPDGAPCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDVTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFDGTPANHTARFTEVVEQYRTDLAD
                     MVGQCAGGSADGVPWICGDTTYFWKQKSESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     ANVPTNKPEEDPDIPASGYYGAASRTSANWTSADRAIHFSSWARRGIISDRLASAILL
                     HAGRTAELMGGEQVVMPPDEKPSPDTPSTPSTDGKSVTTLLYYRATESGGLLNPQGWG
                     AEGGRALVVDDAGAAGGKALRWTKQTGSSSWFMQHDAGNGADLLEKGGLISCRFKVDG
                     TLTANQYALALYWPVSSLPQGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNAGQNT
                     KLGSFGAFDNEWHTLGFRFAGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTD
                     ITSGATYPVLIESITVEVNNP"
     gene            2845..2988
                     /locus_tag="ACHJZ2_004984"
                     /pseudo
     CDS             2845..2988
                     /locus_tag="ACHJZ2_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          897 c          714 g          744 t
ORIGIN      
        1 ctttactctc cccgcgccgc cttacgacgg tcctctctga ttttgaaata caggttagtc
       61 agatacgtca gcaggccaaa cagcagactc cccagcacac ctatcgccac ccactgggac
      121 ggagagactt tgtccagcag ctgcagtaac cagtatcccg tccccaccgc tgacgtggtg
      181 tatgacacac ccgttgtgat tttttccatc tggtacatac cccgtctccc gcaatccgga
      241 agctcacaac aacaggaggg gcatcagctc acaccgacag cccctgcgta tggttacatc
      301 atcatttcgc cgccaggctg aggctcactg ctaccgtcag gctgagacac aacgccatct
      361 gaaacagcac tgtcacccgc gccgtcttca ggctcaggag cagccggtcc ccccagcagc
      421 tcatccagaa tggcatccac ttcagcatca agacgcgcct caagattctg gcggagttgc
      481 tgtttcagtg cgcttctgac ttcttcagag cgcaggacgt ccttcactgc ttcagcagtg
      541 accagagatt ttatttctga catgggattt tctcgttgaa aggtgttgtt aagaaagttg
      601 ctacgaaatg agaggctctt cgggttttgt tccggctgac tggctggcgc tgattttttc
      661 cgccgccgcg gcatcaatct ttttgcgtat atagttccgg ataaccttat acccgccact
      721 taccagatat aacgtgcaca ccaccgtgca gaaatacaat aaaataagct gtacaaatct
      781 cattatccct cccggttatt gatatggtgt tgacatcgtt aatacctgtt ggttaaaaaa
      841 gtgtcctgca tgttttgctt tggatataac gacatttgcc gccggttctg gctccttgtt
      901 ttccctgccc cggcggcctt tttttcctgc ttacgggtta ttcacttcca ctgttatact
      961 ttcaatcagc accggatatg tcgcaccgct agtgatatcg gtcacgcgca atttgtctgc
     1021 cgtaaacgtg ccgaccggtg actgtgacag catgaacggc gtcccgtcct taccatcaat
     1081 gaccggcgtc acctcaatac tgttgttacc ggcaaaacgg aagcccagcg tatgccattc
     1141 gttatcaaat gcgccgaatg accccagctt cgtgttctga ccagcatttc ccttgtggta
     1201 catcacatta aggtctgtgg catcgctctg tacgtaaaac gacgccagca ggttatgacc
     1261 ggcattacct tccagtgtga cgccctgagg cagtgaagaa accggccagt acagcgccag
     1321 tgcgtactga ttagctgtca gtgtgccatc aactttaaaa cgacaactga taagcccgcc
     1381 cttctccagc aggtctgcgc cattaccggc atcatgctgc ataaaccacg aggaacttcc
     1441 tgtctgtttg gtccacctca gcgccttacc tcctgcagca cctgcatcat caactaccaa
     1501 tgcacgccct ccttcagctc cccatccctg cggattcagt aaaccacctg actctgttgc
     1561 acggtaataa agcagcgttg tcactgattt cccgtccgtt gacggtgttg atggtgtgtc
     1621 cggtgacggc ttctcatccg gcggcatcac aacctgttcc ccacccatca gttcagccgt
     1681 ccgtcctgca tggagaagaa tcgctgaggc aagacggtca gaaataatcc ccctgcgtgc
     1741 ccatgagctg aaatggatcg cacggtctgc tgacgtccag tttgccgacg tccgggaggc
     1801 cgcaccgtaa tatcctgatg ccggaatatc cgggtcttct tccggtttgt tcgtcgggac
     1861 atttgctccg ttctcatcgg tcatgaacgg cacaaagtga atattctttt ccgttttgtt
     1921 tttgtaactg ccgtacaccg tctggtaagt ggattcgctc ttctgcttcc agaaataagt
     1981 tgtgtctcca catatccagg gaacaccgtc agcagaacca ccagcgcact gtcccaccat
     2041 atctgcaagg tccgtacgat attgttccac tacttctgta aaacgggctg tgtgatttgc
     2101 tggcgttccg tcaaagtcaa attccccctg catccacacc acggcaagca gcacattttt
     2161 cgggttcttt gccagcgcgg cttttgtacg accgatgaga tccttataca gcggcctgcc
     2221 cacaccccag cgggtagaac tctctgaggc accggtcacg tcactgtatg ttccatctgc
     2281 cccggtggtg aaagctgaac caccacggca gcacggaacc agaagaatac ccgcattcgc
     2341 cggtataaac ggcagcagct ttttggcaat atgcagcccc tgccccacgg ttccgtactg
     2401 acctttcgac aggtcagctt tcggatggtt aagacggctc atgtcctgta catcatgcag
     2461 acagtggtct gccggaataa tgtcgttata tttacaggga gcgccatccg gcgtcactgt
     2521 gctgcgacga gccagttgct ttatacgcgg gtcaggacgg tcatatgtct gcggcagagg
     2581 aagcccctca ccgtaagcca tgccgttcga ctgcccggcc agtggaataa cgtagtaata
     2641 atctggctcc gtggtgacca ctcccggata gccaccatcc cccgtgccgg gcacaaccac
     2701 tggcgtggtc acatccccct ccgcggcaat cgcctgcatc agggtataag gggttatggc
     2761 cacaggacta ccaaacggct gccagccctc tttcagtttg tgtgtcagct tttccgcaag
     2821 atctgacggc gacgccgccc tgacaacatc ataatgttta atcgacatcg aatttctccc
     2881 gtgtacagga acagagttaa aaagccggaa ccggaatcaa atcacaggat gaccatctgc
     2941 cagtggctgg tcgtaaaaaa aaggccgcgc catgcgcagc cggaaataaa gggataacga
     3001 tgatagtttg agaaaaacag aaataacact tttgtggcaa agc
//
LOCUS       DAZFBR010000244         3014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFBR010000244 DAZFBR010000000
VERSION     DAZFBR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.247"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..572)
                     /locus_tag="ACHJZ2_004985"
     CDS             complement(<1..572)
                     /locus_tag="ACHJZ2_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO4570911.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQA"
     gene            complement(668..856)
                     /locus_tag="ACHJZ2_004986"
     CDS             complement(668..856)
                     /locus_tag="ACHJZ2_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4570912.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            complement(853..1041)
                     /gene="dicB"
                     /locus_tag="ACHJZ2_004987"
     CDS             complement(853..1041)
                     /gene="dicB"
                     /locus_tag="ACHJZ2_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4570913.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            complement(1521..1673)
                     /locus_tag="ACHJZ2_004988"
     CDS             complement(1521..1673)
                     /locus_tag="ACHJZ2_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4570914.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            complement(1948..2592)
                     /locus_tag="ACHJZ2_004989"
     CDS             complement(1948..2592)
                     /locus_tag="ACHJZ2_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO4570915.1"
                     /translation="MESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
     gene            2690..2917
                     /locus_tag="ACHJZ2_004990"
     CDS             2690..2917
                     /locus_tag="ACHJZ2_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4570916.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
     gene            2914..>3014
                     /locus_tag="ACHJZ2_004991"
     CDS             2914..>3014
                     /locus_tag="ACHJZ2_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4570917.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYF"
BASE COUNT          791 a          681 c          682 g          860 t
ORIGIN      
        1 tctgcctgaa tttcaggcca tttagcgcca ggctcacatt tatgcttaac ccacccgatg
       61 gcatgcagtt taagttcagg atacatggcg ttaacttctg gcattttcat caacgcttca
      121 acgatatgtc cgccgaatgt tgccatgtct tcctgtagta attcctgtgc gctaatcacc
      181 atatcaacgg tgatgttttc gcatgtgtcg aatttaacca ggactgcgtt ctgtacttca
      241 ggggacagct tgtcaaaatt gacgttcatc ggatcggatt ctggttcgac cggtacaaag
      301 gaagctgact cctcatccca gcggttttcc tgcatatatt cggtatccca ggaatcgagg
      361 gcagggcggg gtatgccggg tttatcctcg cagacaataa atttataagc gcagtcctga
      421 gctgccggga attgctccag aaattgccag tgaaattttg cgcgtgcgcg acgctcatca
      481 ccggcttcaa tggcagtggc caccgcaaca gcgctatctt cttttatggc ctgttcgtca
      541 ggaatagcag cgcaaataaa gattttactc attgtgtttt aacctcatta cagatttcag
      601 ggtgaacgaa tccctgccat tgctggcatt tttaatccgt tggtatggcg ttaatatggc
      661 tggcgggtta tccagccggt gtttcgttat tcaggtacag cgatactttt tttaccggta
      721 ggcattcacc agaaattttt tgctcgtctc ttgcctggag gcaggattct ttactggcat
      781 aaattccggt aataacattc tgtgattcac ccgttataag aaaaaccgtc atcaccagtg
      841 caaatgctga agtcattgac gttctccgaa aataccaagt ttaataaggg caattcggga
      901 aagaatggaa ttatcattga gcagataagg ctcatatttc cgcatattaa tggcatcctc
      961 agtaaacgct ttattactga gcagaacacc aatatcaaaa caaccttcag acgtattaac
     1021 gtttggtaat aacgtttcca ttatcgcgtc ctcaacaatg aattttgtga tgcagtgcct
     1081 ggtgcctcca ggtgacgtta accagttaac aattaacgtc gggttagttg atgctcgtta
     1141 cgccagtaaa agaccgcctt actgctttaa ctgttccgcg tgcgcatagc cgcattcacc
     1201 gcatcacaaa attcacttta aaaagggcgg atatccattt ccgccgaatc accagaaaag
     1261 tgataacaga ggttgttgtg gctggggtgt cacttaagcg tatggtcaac ctgacaaccc
     1321 ggtgtcctca acgggggaag gaataacccc gccatactta ccgccgcgcc atttcgcgga
     1381 ttgccacaac cagaagcgca cggtcgaaga aatttaacgg caagacttat atgcaaaggg
     1441 atctcgccgt gcgctttcgt gttatgccct gacttttcag ggtaaattaa cctgtggaaa
     1501 cctgttttta ctggcgctaa tcagttagcg tttcttgcta accagcgatg cgcgccagct
     1561 tcggttttaa acgttttact tctggtatac gtcatcgcgg taaacgtgcc gtcgtggttg
     1621 gggaatactc cgcacaccag agattcattg ttgccaagat tgagcatatc catgttgacc
     1681 tcatttaccc ttaacgccgg gtcgcggaac taaaaacctg ctgcgctgtt atacaaagtg
     1741 ttcccgccgt catgttcata cgcctcgggc tggctactta acccctgacc actgccgggt
     1801 aactcgaggt attgcccgac gttctgtggg gcgaggtagg gtggtatgta ataaatctaa
     1861 aagtatttag tttttcagtc aaggtaaatc tagattaatt taaaaaaaag gtcggcattg
     1921 ccgacctcaa gtaaaggacg tggcgggtta taagttgaac tgtacacctt ttgctacggc
     1981 aacaatttta cactctggtg taagtaagga tgactgatag cgtggattga gagggcttaa
     2041 atacacaagc ttcccatcaa taactaattt ctttatagtc atagatggtt catttttaag
     2101 cggatccgga actattacag cgacgatgct gccattttta taactttctc ctggccttag
     2161 gatcacagta gcaccaactg ggatactcgg tgatcctgat gggttatgca ttgtgtcatc
     2221 aggcattgaa acggcaaaat caccttcctc gacatcaaag aatgtggtga ttctatcgac
     2281 atttcccatt gttttctctc cttttaagat taggaaagaa atcgcctcac cccacgaaag
     2341 gtaggggatc ttggtgcctg aggtactttg tacaaaagat aattcaggag acgatactcc
     2401 atataggaga taggactcag tagttcctaa tgcctgggct aacttactta gagctttgct
     2461 acctggttcg tttagatctt tctcccagta ccctatagta accccagtca cgcctgaaag
     2521 cttacccagt tctacttggg tcagcccctt atcttttcta agtctcttaa gcctgaagcc
     2581 aaggctttcc atcattttct cctgcaaatt gaatataaat tattttagat tgcattgacc
     2641 taaaaaaaat tatcttgtaa tctaaaaatg cttagattta ggaggtaaaa tgcgagttga
     2701 tgaacttgtc cagttttttg gttctgttca gagggttgcc gatttttatg ggataacccg
     2761 cgaagctatt tacatgtggc gtaagcgtcc tggtgaaata gttccgaagg ggagggctgc
     2821 ggaagctgct gcatactcca aggggaaatt atctttgaac ccagaacttt acaaaaagaa
     2881 ggataccact cagggcgaag gaaaggggga ttcatgaaaa tcaaacatga gcacatccgc
     2941 atggcgatca atgcctgggc ctatccggac ggtgagaaag ttcctgcagc tgaaatagcc
     3001 cggacttatt tcga
//
LOCUS       DAZFBR010000245         2888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFBR010000245 DAZFBR010000000
VERSION     DAZFBR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.248"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            568..774
                     /locus_tag="ACHJZ2_004992"
     CDS             568..774
                     /locus_tag="ACHJZ2_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO4570918.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNR"
     gene            850..1146
                     /locus_tag="ACHJZ2_004993"
     CDS             850..1146
                     /locus_tag="ACHJZ2_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4570919.1"
                     /translation="MNAYYIQDCLEAQSWARYYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1152..1937
                     /gene="bet"
                     /locus_tag="ACHJZ2_004994"
     CDS             1152..1937
                     /gene="bet"
                     /locus_tag="ACHJZ2_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO4570920.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1934..2611
                     /locus_tag="ACHJZ2_004995"
     CDS             1934..2611
                     /locus_tag="ACHJZ2_004995"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO4570921.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            2611..2793
                     /locus_tag="ACHJZ2_004996"
     CDS             2611..2793
                     /locus_tag="ACHJZ2_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO4570922.1"
                     /translation="MKHPHDNIRVGTITFAYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            2766..>2888
                     /locus_tag="ACHJZ2_004997"
     CDS             2766..>2888
                     /locus_tag="ACHJZ2_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4570923.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLA"
BASE COUNT          788 a          713 c          715 g          672 t
ORIGIN      
        1 gatggcgggg ctaataagtc atttatgtat catttagcaa ctgactaaaa aaatacacat
       61 aagaactcag actaccaata ttcgattaaa tttgctgcca acctaccgag ttcaccacaa
      121 cccgatgccc ctctgtcacg aggcacatta aagctgtaca aatttcagcc acaccaataa
      181 gtcttcatac tcttttttat aaaaattcat ttaattatca atcagaacaa gtatatcaaa
      241 cattatcaat actaattgct attgactaca ccaatagcca ttgctattgt catgctattg
      301 catcgatgtt ggtatcacaa aatttataac ttcaccgttg cgatgaccgc ttagatccgc
      361 agcttgaatt tcagcaggct ccggggagtg cgaggggtga agcggacgcg tgaacgtcgg
      421 tgtgaccagc tgaaatcaac tcaacacttc atacctcagt cgcttcaacg aggcgactta
      481 gttatgacaa ccggcggcca tccaccgcct gaatacgcgc agaagtctct atatgttcag
      541 cagcccagct tacgggcagg agtttttatg gttcatcaac attacggaac gcagaccgtt
      601 aatcgaggtg cggtcatgcc aggaatgctg gtcaaacaca aagatggtac ctggactgca
      661 tcagctaatt tacgcggacg gctttatctg catcgcggca tcgagcgcac ttatacccgt
      721 gatttgctcg tggaagtttt tctcgacgga cgcggtaacg gcctgaatcg ctaatcccct
      781 ttcctgtttt cctaatcagc ctggcatttc gcgggcgata ttttcacagc cattttcagg
      841 agttcagcca tgaacgctta ttacattcag gattgtcttg aggctcagag ctgggcgcgt
      901 tactaccagc agatcgcccg tgaagagaaa gaggcagaac tggcagacga catggaaaaa
      961 ggcctgcccc agcacctgtt tgaatcgcta tgcatcgatc atttgcaacg ccacggggcc
     1021 agcaaaaaag ccattacccg tgcgtttgat gacgatgttg agtttcagga gcgcatggca
     1081 gaacacatcc ggtacatggt tgaaaccatt gctcaccatc aggttgatat tgattcagag
     1141 gtataaaacg gatgagtaca gcactcgcaa cgctggctgg gaagctggct gaacgtgtcg
     1201 gcatggattc tgtcgaccca caggaactga tcaccactct tcgccagacg gcatttaaag
     1261 gcgatgccag cgatgcgcag ttcatcgcat tgttgatcgt cgccaaccag tacggcctta
     1321 atccgtggac gaaagaaatt tacgccttcc ctgataagca gaacggcatc gttccggtgg
     1381 tgggcgttga tggctggtcc cgcatcatca acgaaaacca gcagtttgat ggcatggact
     1441 ttgagcagga caatgaatcc tgcacatgcc ggatttaccg caaggaccgt aatcatccga
     1501 tctgcgttac cgagtggatg gatgaatgcc gccgcgaacc attcaaaacc cgcgaaggca
     1561 gagaaatcac ggggccgtgg cagtcgcatc ccaaacggat gttacgtcat aaagccatga
     1621 ttcagtgtgc ccgtctcgcc ttcggatttg ctggtatcta tgacaaggat gaagccgagc
     1681 gcattgtcga aaataccgca tacactgcag aacgtcagcc ggaacgcgac atcactccgg
     1741 ttaacgatga aaccatgcag gaaattaaca ctctgctgat cgccctggat aaaacatggg
     1801 atgacgactt attgccgctc tgttcccaga tatttcgccg cgacattcgt gcatcgtcag
     1861 aactgacaca ggccgaagca gtaaaagctc ttggattcct gaaacaaaaa gccactgaac
     1921 agaaggtggc agcatgacac cggacattat cctgcagcgt actgggatcg acgtgagagc
     1981 tgtcgaacag ggagatgatg cgtggcacaa attacggctc ggcgtcatca cagcttcaga
     2041 aattcacaac gtaatagcaa aaccccgatc aggaaagaag tggcctgaca tgaaaatgtc
     2101 ctacttccac accctgctgg ctgaggtttg caccggtgtg gctccggaag ttaacgctaa
     2161 ggctctggcc tggggaaaac agtacgagaa cgacgccaga accctctttg agttcacttc
     2221 cggcgtgaat gttactgaat ccccgatcat ctatcgcgac gaaagtatgc gcaccgcctg
     2281 ctctcccgat ggtttatgca gtgacggcaa cggccttgag ctgaaatgcc cgtttacctc
     2341 ccgggatttc atgaagttcc ggctcggtgg tttcgaggcc ataaaatcgg cttacatggc
     2401 ccaggtgcag tacagcatgt gggtgacacg aaaagatgcc tggtactttg ccaactatga
     2461 cccgcgcatg aagcgtgaag gcctgcatta tgtcgtgatt gagcggaatg aaaagtacat
     2521 ggcgagtttt gacgagatgg tgccggagtt catcgaaaaa atggacgagg cactggctga
     2581 aattggtttt gtatatgggg agcaatggta atgaagcatc ctcacgataa tatccgggta
     2641 ggcacgatca ctttcgccta ctccgttaca aagcgaggct gggtatttcc cggcctttct
     2701 gttatccgaa atccactgaa agcacagcgg ctggctgaag agataaataa taaacgaggg
     2761 gctgtatgca caaagcatct cctgttgaat taagaacgag tatcgggatg gcacatagcc
     2821 tcgctcaaat tggagtcagg tttgtgccaa taccagtaga aacagacgaa gaatttcata
     2881 cgttagcc
//
LOCUS       DAZFBR010000246         2873 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFBR010000246 DAZFBR010000000
VERSION     DAZFBR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.249"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            33..1238
                     /gene="yedE"
                     /locus_tag="ACHJZ2_004998"
     CDS             33..1238
                     /gene="yedE"
                     /locus_tag="ACHJZ2_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO4570924.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            1235..1468
                     /gene="yedF"
                     /locus_tag="ACHJZ2_004999"
     CDS             1235..1468
                     /gene="yedF"
                     /locus_tag="ACHJZ2_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO4570925.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1577..2245
                     /locus_tag="ACHJZ2_005000"
     CDS             1577..2245
                     /locus_tag="ACHJZ2_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO4570926.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            2356..2835
                     /locus_tag="ACHJZ2_005001"
     CDS             2356..2835
                     /locus_tag="ACHJZ2_005001"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4570927.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
BASE COUNT          643 a          712 c          764 g          754 t
ORIGIN      
        1 tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc aaacacgcct
       61 ggttgattaa attctgggcg cccatccccg cggtcatcgc ggcgggtatt ctctccactt
      121 actattttgg catcactggc accttttggg ctgtcacggg tgaatttacc cgttggggcg
      181 gtcagctcct gcagctgttc ggcgtccatg ctgaagagtg gggttatttt aaaattatcc
      241 atctggaagg atcgccatta acccgcatcg acgggatgat gatcctcggt atgtttggcg
      301 gctgctttgc cgcagcgctg tgggccaaca atgtcaaact acgcatgccg cgtagccgta
      361 tccgcattat gcaggccatt attggcggca ttatcgccgg ttttggcgcg cgtctggcaa
      421 tgggctgtaa cctggcggca ttctttaccg gtattcctca gttctcgctg catgcctggt
      481 tctttgccat cgccactgcc attggttcat ggtttggcgc gcgctttacc ctgctgccca
      541 tcttccgtat tcccgtgaaa atgcagaaag ttgctgccgc ctcaccgctg acgcaaaaac
      601 cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggc atgctgggct
      661 gggcgctgct cacagcgatg aaccaaccca aactggggct ggcaatgctg tttggcgtcg
      721 gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt
      781 ggatcaccgg acgtacccat atggcgaaag caatcattat tggtatggcg gtgagtgcca
      841 tcgggatctt cagttacgta cagttaggcg ttgaacccaa aatcatgtgg gcgggaccaa
      901 acgcggtaat tggtggttta ctgtttggtt ttggcatcgt gctggctggc ggctgcgaaa
      961 ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca
     1021 atgtgatcgg ctcaacgatt ctggcgtatt actgggatga tttcgctccg gcgctggcca
     1081 ccgactggga caaaatcaac ttgctgaaaa cctttggtcc gatggggggc ctgctggtga
     1141 catatttgct gttgtttgct gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc
     1201 gccgtgcggc accgcagact gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg
     1261 ccttgatatg gtgggtgaac cctgccctta tccggcagtc gcaacccttg aggcgatgcc
     1321 gcagttaaaa aaaggggaaa tcctggaagt ggtgagcgac tgtccgcagt cgatcaataa
     1381 tattccactg gatgcgcgta atcacggcta tacggtgctg gatattcagc aagacgggcc
     1441 gaccattcgt tatttaattc agaagtaatc cctcattccc gctgtgaccc acagcgggaa
     1501 catcgttacc tttgtatcac atagatttta cccgccccct ctcctattct tttcagtatg
     1561 atctcaggag gtttctatgt gtggacgctt tgcccaatcc caaacgcgtg aagattacct
     1621 tgcgcttctc gcggaagata ttgaacgcga tattccctat gatcccgaac ccattggcag
     1681 atacaacgtc gcgccgggaa ccaaagtcct actgctcagt gaacgtgatg aacaccttca
     1741 tctggatccg gttttctggg gatatgctcc cggatggtgg gataaaccgc cgctgattaa
     1801 cgcccgcgta gaaactgcgg caaccagtcg tatgtttaaa ccgctctggc aacatggtcg
     1861 ggcaatctgt tttgccgatg gctggtttga gtggaaaaaa gaaggcgaca aaaagcagcc
     1921 ttattttatc tatcgtgctg atggacaacc tgtttttatg gcagcgatag gcagcacacc
     1981 gttcgagcgc ggtgacgaag ccgaaggatt tttgattgtc actgcagcag cagaccaggg
     2041 tctggtagat attcatgacc gccgcccgct ggttctgtcg ccagaagctg cgcgggaatg
     2101 gatgcggcaa gagattagcg gtaaagaagc ctcagaaatc gcggccagtg gctgtgttcc
     2161 agcaaaccag ttctcctggc acccggtatc gcgcgcggtg ggtaatgtta aaaaccaggg
     2221 tgcggagtta attcaacctg tttgataagc ctggagatta ttgatcaaca atactgcgtc
     2281 ataagtaatc tctattagac aaagatttca ttacctgttg gcatattgca aaaataacac
     2341 caatacggaa tcgtcatgtt cacgattaaa acagatgatc tcacccatcc agcagtgcaa
     2401 gcattagtgg cttaccatat ttccggcatg ctgcagcagt ctccccctga aagcagtcat
     2461 gctttagacg tgcaaaaatt acgtaacccg acagtgacat tctggtcagt atgggaaggc
     2521 gaacaactcg caggaattgg tgcgctgaag ttgctggatg ataaacatgg cgaactgaaa
     2581 tcaatgcgga ccgcgccaaa ttatttacgt cggggtgtcg ccagtctgat tttacgccac
     2641 attttgcagg tcgcccatga cagatgcctt catcgcctga gtttagaaac gggtacacag
     2701 gctggattta cggcctgcca tcaactttat ttgaagcatg gtttcgttga ttgcgaaccg
     2761 tttgccgatt atcaacttga tccacacagt cgatttttgt cattgacgct atgcgaagat
     2821 aatgagttgc tttgagccag acgcagcaca ttcttgcatt cgacgtgctg cgt
//
LOCUS       DAZFBR010000247         2854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFBR010000247 DAZFBR010000000
VERSION     DAZFBR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.250"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..203)
                     /locus_tag="ACHJZ2_005002"
     CDS             complement(<1..203)
                     /locus_tag="ACHJZ2_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4570928.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFP"
     gene            complement(355..954)
                     /locus_tag="ACHJZ2_005003"
     CDS             complement(355..954)
                     /locus_tag="ACHJZ2_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4570929.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFYGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRSDAFIVGIGYRF"
     gene            complement(1022..>2854)
                     /locus_tag="ACHJZ2_005004"
     CDS             complement(1022..>2854)
                     /locus_tag="ACHJZ2_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4570930.1"
                     /translation="TLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQH
                     VPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGV
                     RFSLRLTSGKGTDARLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTF
                     RIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETAARYLG
                     SALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGEIGKT
                     HLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQTVNKKLENQSAAIQQIQKVQVDT
                     NNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTK
                     PMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSG
                     TLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQP
                     FDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMP
                     AGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT          644 a          835 c          687 g          688 t
ORIGIN      
        1 ggaaatccct ccaccaacag aatgacgctg tactgaccgt actcaacatc catgctgtaa
       61 cgtccggctt catccggatt ttcagaggcc accgtgttca ccaccaccgt gctgctggtt
      121 cgtctggcct tcagcacaat ggtgcagttc tgtactggtt ttcctgtgcc atctttaagc
      181 acgccagaaa tttttactgt catacttttc caccaataaa aaaagcccgc agcagtgacg
      241 ccacgggctt caggacagtg taactttacg tttcctcaaa cgcagttcac cccataaggt
      301 ggatgaacct gcgtatcata acaatattta cagaagataa atcggcgtct gttgtcagaa
      361 acggtatccg ataccaacaa taaatgcatc cgatcgccag tcgccactac cggaaccttc
      421 ataagcaagg tcaatggtca cggattcggt cgggttaaac tgcacgccag ccccccacgc
      481 cagagacgtg ttgctgtggc gaccgtcatc acttccggtc agcacatcgt gcgttttccc
      541 cttgttgtca gttacgcgga gataatcccc gtagaaagtc gacacacggc tgtaagccac
      601 acccgccatc gcatacgcgc tgaaccattc attcacgcgt acagacggcc ccgccatcac
      661 gctgaaccag cggttacgca cggaatcttc atgccagcgg gtatcgctgt agcgcgtttt
      721 ttgctcatcc tcagcattgg cataactgaa ggacgtaatc agccccagcg cgtccataaa
      781 ctcataacgg tatttcacgt taatcccgtt cagatcatca ctaccgggaa cgttcgtcga
      841 ggcatggaga taccccgcgc tcagcgtgga ctgatgttct gctgcactcg ctggcgtagc
      901 agcggcgacc tgccagacta ctgcggacaa aataacagca cataatttac gcataattac
      961 ctctcgcttt tctgcaataa aaaaggcgcc atttctggcg cccgtatctg ggttataaaa
     1021 ttcagctaat cgtgatgcct gcagtggctt tcttcatcac cacaaccagc aaatcgctga
     1081 tacttgctgt gggataccag ttatttacca gccatgctga caccgaaaac tccagtgtca
     1141 tgtgaccgtg accggcaggc atatcaataa caccactgta aatcagcgta ttatccagcg
     1201 cggtacggtt ataaatttca gcaccgtttt tccgcactat cagacggcat gaggagtaaa
     1261 tatcagtatg ctctttctca tgtttagcgc cgctgaatgc caccgccgga ataacaatct
     1321 gccggtcaaa cggctgatcg tcataaaccc tgacggtaat ggttcctgat ggccaccgct
     1381 ccggtgcacg ggagtcccgg gggaaagctt tgcccactgt tttaacgaga tcgccttcaa
     1441 tctggttcgc ggacaatttt cccagaaccc gacagttctc gttaatcgtg acgttgttga
     1501 gcgtcccgga gttcgcattc acgttaccgc tgatatcggc atttttcgcc gtcagccgcc
     1561 cgtccggtgt cagggaaaat gccggaggat taccgccgct ggtaatggtg ggagccgtca
     1621 gatatttcag gaacacttca ttcataaata tctgatcgcc ctgaccaaca aacatcggct
     1681 ttgtgttgcc attcgcagga ttaatcatcg caatcctgtc tgccgccagc agcacctgac
     1741 tctgcattcc tgctggcgta ttctcaatac cggcaccgat acccgcaata taaaggcgtc
     1801 cgtcctgcat ctgctgcagt ttcacggccc acatgctgtt caggttatta tttgtatcaa
     1861 cctgaacttt ctgtatctgc tggattgccg cactctgatt ttccagtttt ttattgacgg
     1921 tctgcgtgat ttcattgctg acattcgtaa tggacgtcct gatttcagcc aggtccggcg
     1981 caagctgacc gttatcaatc tgcgtccaca gctcctgggc cagatgtgtt ttcccgattt
     2041 ctcctttgaa aaaatccagg taaccttccg catcatcgct cgcccgaccg acagcctcca
     2101 cgaatgccga tttgccaaca gtattcacac tgcggatata aaaataataa tcatggcccg
     2161 gtttgatatt gatactggca gctatccagt acagcgccga gccaagatag cgggctgcgg
     2221 tttcaacctg cctgatatcc gcaatccgct tttccgagaa ccagaactca aactgtaccg
     2281 tcgggtcata aacggcaaga tgcggcgtgg cggttatctg aaaatagccc ggcgtcagct
     2341 caatccgcga cggtgctgcc ggtgcggcaa tccggaaggt ggtggtggca ggttcaccct
     2401 gctggccata gctgtttatc gcccgcaccg tcagggtgta ttccccgagc ggcaggccgc
     2461 tgaaacggtg ctccgtgtct gcggtgatgg cggtggtcac cagtctggca tccgttccct
     2521 taccactggt caggcgcaga ctgaagcgca cgcccttcac cacccgcggc gtgtcccatt
     2581 tagcctgcgc cagatactgg ccgtcagctg cactcacctc caccgtcagg tgctgtactg
     2641 ccggtgggat gacgctgttc agggaacctg actgcggctc aaagcgggca ccgttatcca
     2701 cgatggcttc tttttccggt acgtgctgca ccgccgtgat ggcaaaggtg ccgtccgtgt
     2761 tttcccggac ggagacacag cggaacaggc gacggcgcag tgacggcagg gagagtcccc
     2821 acaccccgta tgtctccaca ccatcaggca gggt
//
LOCUS       DAZFBR010000248         2839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFBR010000248 DAZFBR010000000
VERSION     DAZFBR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.251"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            138..2417
                     /gene="ydeP"
                     /locus_tag="ACHJZ2_005005"
     CDS             138..2417
                     /gene="ydeP"
                     /locus_tag="ACHJZ2_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO4570931.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAQNTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSVEFLARLGERYGFTPPHVPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2665..>2839
                     /gene="safA"
                     /locus_tag="ACHJZ2_005006"
     CDS             2665..>2839
                     /gene="safA"
                     /locus_tag="ACHJZ2_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO4570932.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVILLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHF"
BASE COUNT          766 a          655 c          687 g          731 t
ORIGIN      
        1 taacgataat agattaaaaa tagaaagaga gtagtcccct actcttatat atccatgttg
       61 gcgataatcc ctttgtatgt actgtgcatc atcgctatta caaatcctaa taattcattt
      121 ccacacagga taagtagatg aagaaaaaaa ttgaatccta ccagggtgct gccggtggtt
      181 ggggtgctgt taaatccgta gcgaatgcag tacgtaagca gatggatata cgccaggatg
      241 ttattgccat gtttgacatg aataagccag agggctttga ctgtccgggt tgtgcatggc
      301 cagatcctaa gcacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa gcaatcgcct
      361 gggaagtcac ggataagcag gtaaatgcct ctttctttgc tcagaatacg gttcaatcat
      421 tacttacctg gggagatcac gagcttgagg ctgcgggacg tctcactcag cctttgaaat
      481 atgatgctgt cagcgactgt tacaagccat taagctggca acaagctttc gacgaaattg
      541 gcgcacgcct tcaaagctat agtgatccca atcaggttga attctatact tcgggccgca
      601 cttccaatga agctgccttt ctttatcagc tttttgcccg tgaatacggg agcaataact
      661 ttcccgactg ctccaacatg tgccatgaac cgacaagcgt gggtttggca gcgagtatcg
      721 gtgtaggtaa agggaccgtg ttgctggaag actttgagaa gtgcgattta gtcatttgca
      781 ttgggcataa ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc gctttagtga
      841 aacggggagc gaaaatgatc gccatcaatc ctctacagga acgtggcctg gagcgattta
      901 ccgcaccgca aaacccgttt gaaatgctga cgaactctga gactcagttg gccagtgcct
      961 actataacgt gcgcattggt ggtgatatgg cgttgctcaa ggggatgatg cgcctgttaa
     1021 ttgagcgcga tgatgctgca agcgccgcag gtcggccctc attgctggat gacgaattta
     1081 ttcaaacgca taccgtcggc tttgacgagc tacgccgtga cgtgctcaat tccgagtgga
     1141 aagatatcga acgtatttct ggactaagtc agacacaaat cgccgaactg gctgatgcat
     1201 atgccgctgc cgaacgaacc attatctgtt acggaatggg gatcactcag cacgaacatg
     1261 gtacccagaa tgtacagcaa ctggtcaatc tgctgttgat gaaaggtaac attggcaagc
     1321 ctggtgcggg tatctgccca ctacgtggcc actctaatgt acagggcgac cgaaccgtcg
     1381 gtatcaccga gaaaccgtct gtagagtttc tggctcgcct gggtgagcgc tatggcttca
     1441 ccccacctca tgtacctgga catgctgcaa ttgccagcat gcaagcaata tgtacggggc
     1501 aggctcgagc attgatctgc atggggggca actttgcact ggcaatgcca gatcgggaag
     1561 cgagcgctgt accgttaacg caattagatt tggcggtaca cgtagccact aagcttaacc
     1621 gctctcatct gctgaccgca cggcatagct atattctgcc ggttctggga cgtagcgaga
     1681 ttgacatgca aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg tcgatgattc
     1741 atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct gaaatcagag tgtgctgtgg
     1801 tcgcgggaat cgcgcaggca acactacccc agagcgtggt agcctgggag tatctggtgg
     1861 aagattatga tcgcattcgc aatgacattg aagctgtgct gccagagttc gccgactata
     1921 accaacgcat ccgtcatccc ggtggttttc acctgataaa tgcagctgct gaaaggcgct
     1981 ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa agggctgtta gaagatccct
     2041 cttcagcgtt taacagtaag ctggtcatgg cgacagtacg cagccacgat cagtacaaca
     2101 cgacgattta tggtatggat gatcgctatc gaggggtatt cggtcaacga gatgtggtct
     2161 ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga gttaatctta
     2221 ttgcgcttac gccagacggt aagcgcagct cacgccgcat ggatagatta aaagtggtca
     2281 tttaccctat ggctgaccgc tcactggtga cctattttcc agaatcgaat cacatgctaa
     2341 cacttgataa ccacgatcca ttaagtggca ttcctggcta taaaagtatt ccggttgaat
     2401 tagaaccatc aaattaatgc ctcttctcat ttcttctgct gtcatccgca cagcagaaga
     2461 attcctcatt gattattatc gcgcaatttg ctcacatgga ttaaattaaa ctacatacta
     2521 taagatataa acttctgcct acagctgtaa gaaactccgc tcagtactga agcaccagtc
     2581 ctatttcctc ttttctcaag cctgttatat taagcatact gattaacgat ttttaacgtt
     2641 atccgctaaa taaacatatt tgaaatgcat gcgaccacag tgaaaaacaa aatcacgcaa
     2701 agagacaact ataaagaaat catgtctgta attgtggtta tcttattact gacccttacg
     2761 ttgatagcca ttttttcggc aattgatcag ctgggtattt cagaaatggg tcgcattgca
     2821 agagatctta cacatttca
//
LOCUS       DAZFBR010000249         2801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFBR010000249 DAZFBR010000000
VERSION     DAZFBR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.252"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..570
                     /locus_tag="ACHJZ2_005007"
     CDS             <1..570
                     /locus_tag="ACHJZ2_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4570933.1"
                     /translation="KTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQL
                     HETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMT
                     GILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYG
                     EMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            631..>2801
                     /locus_tag="ACHJZ2_005008"
     CDS             631..>2801
                     /locus_tag="ACHJZ2_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4570934.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTP"
BASE COUNT          604 a          738 c          921 g          538 t
ORIGIN      
        1 aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggtgtt tcgtcagaag
       61 ctgagcgacg gctggtatca ggtacggatt accgggcggg acgtcagcac gtccgggtta
      121 acggcgcagt tacatgagac tctgcctgat ggcgctgtga ttcatattgt tcccagagtc
      181 gccggggcca agtcaggtgg cgtattccag attgtcctgg gggctgccgc cattgccgga
      241 tcattcttta ccgccggagc cacccttgca gcatgggggg cagccattgg ggccggtggt
      301 atgaccggca tcctgttttc tctcggtgcc agtatggtgc tcggtggtgt ggcgcagatg
      361 ctggcaccga aagccagaac tccccgtaca cagacaacgg ataacggcaa acagaacacc
      421 tatttctcct cactggataa catggttgcc cagggcaatg ttctgccggt tctgtacggt
      481 gaaatgcgcg tggggtcacg cgtggtttct caagagatca gcacggcaga cgaaggggac
      541 ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt tttatgtgaa accgcctccg
      601 ggcggtttta tcgtttatgg agcatgacga atgggtaaag gcagcagtaa ggggcatacc
      661 ccgcgcgaag cgaaggacaa cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc
      721 agcgaagggc cggttgaagg tccggtggat ggattaaaaa gcgtgctgct gaacagtacg
      781 ccggtgctgg acagtgaggg gaataccaat atatccggcg tcacggtggt gttccgggcc
      841 ggtgagcagg agcagacacc gccggaggga tttgaatcct ccggctccga gacggtgctc
      901 ggtacagaag tgaaatatga cacgccgatc acccggacca tcacgtcggc aaacattgac
      961 cgtctgcgtt ttactttcgg cgtgcaggca ctggtggaaa ccacctcaaa gggggacagg
     1021 aatccatcgg aagtccgcct gctggttcag atacaacgta acggtggctg ggtgacggaa
     1081 aaagacatca ccattaaggg taaaaccact tcacagtatc tggcctcggt ggtggtggat
     1141 aacctgccgc cgcgcccgtt taatatccgg atgcgcagga tgacgccgga cagcaccaca
     1201 gaccagctgc agaacaaaac gctctggtcg tcatacaccg aaattatcga tgtgaaacag
     1261 tgctacccga acacggcact ggtcggcgtg caggtggact cggagcagtt cggcagccag
     1321 aaggtgagcc gtaattatca tctgcgcggg cgtattctgc aggtgccgtc gaattataac
     1381 ccgcagacgc ggcaatacag cggtatctgg gacggaacgt ttaaaccggc atacagcaac
     1441 aacatggcct ggtgtctgtg ggatatgctg acccatccgc gctacggcat ggggaaacgt
     1501 cttggtgcgg cggatgtgga taaatgggcg ctgtatgtca tcggccagta ctgcgaccag
     1561 tcagtgccgg acggctttgg cggcacggag ccgcgcatca cctgtaatgc ctacctgacc
     1621 acacagcgta aggcgtggga tgttctcagc gatttctgct cggcgatgcg ctgtatgccg
     1681 gtatggaacg ggcagacgct gacgttcgtg caggaccgac cgtcggataa ggtgtggacc
     1741 tataaccgca gtaatgtggt gatgccggat gatggcgcgt cgttccgcta cagcttcagc
     1801 gccctcaagg accgccataa tgccgttgag gtgaactgga ttgacccgga taacggctgg
     1861 gagacggcga cagagcttgt ggaggacacg caggccattc tccgttacgg tcgtaacgtc
     1921 acgaagatgg atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg
     1981 ctgattaaaa cggagctgct ggagacgcag accgtggatt tcagcgtggg cgcagaaggg
     2041 cttcgccatg taccgggcga tgtcattgaa atctgtgatg atgactatgc ggggatcagc
     2101 atcggtgggc gtgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa
     2161 atcacgctgc catcctccgg taccacgctg ataagcctgg ttgacggaag tggcaatccg
     2221 gtcagcgtgg aggtccagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct
     2281 gacggcgttg ccggatacag cgtatggggg ctgaagttgc cgacgttgcg ccagcgcctg
     2341 ttccgctgcg tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag
     2401 catgtacccg aaaaagaagc catcgtggat aacggggcgc actttgacgg cgacctgagc
     2461 ggcacggtga atggcgtcac gccgcccgcg gtgcagcacc tgactgccga agtcaccgca
     2521 gacagcgggg aatatcaggt gctggcgcgc tgggacacgc cgaaggtggt gaagggggtg
     2581 agcttcctgc ttcgcctgac cgtggcagcg gacgatggca gtgagcggct ggtcagtacg
     2641 gccaggacga cggaaaccac ataccgcttc acgcaactgg cgctggggaa ctacaggctg
     2701 actgtccggg cggtaaatgc gtggggacag cagggcgatc cggcatcggt atcgttccgg
     2761 attgccgcac cggcagcgcc gtctcggatt gagctgacac c
//
LOCUS       DAZFBR010000250         2801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFBR010000250 DAZFBR010000000
VERSION     DAZFBR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.253"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..538
                     /gene="escR"
                     /locus_tag="ACHJZ2_005009"
     CDS             <1..538
                     /gene="escR"
                     /locus_tag="ACHJZ2_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO4570935.1"
                     /translation="VLGILKNALGIQQVPPNMALTSVSLILTMFIMSPIILQINDNIS
                     QEPINYTDSDFFQKVDEKILSPYRGFLEKNTEKDNVEFFERAAQKKLGNETILKKDSL
                     FILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTISIPFKILL
                     FILVGGWQKLFEFLLVVN"
     gene            538..807
                     /gene="escS"
                     /locus_tag="ACHJZ2_005010"
     CDS             538..807
                     /gene="escS"
                     /locus_tag="ACHJZ2_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO4570936.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            807..1583
                     /gene="escT"
                     /locus_tag="ACHJZ2_005011"
     CDS             807..1583
                     /gene="escT"
                     /locus_tag="ACHJZ2_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO4570937.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGIQSILDKLYLSYEILPLQAD
                     IAFNRALIDFLFSLWDSFIKLMLSFSVPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            1576..2613
                     /gene="escU"
                     /locus_tag="ACHJZ2_005012"
     CDS             1576..2613
                     /gene="escU"
                     /locus_tag="ACHJZ2_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO4570938.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GMSFFVDIVGLVNTTIDSLNRPFLYAIREILGAVLNIFLLYILPISLIVFVGTVTTGV
                     SQIGFIFAVEKIKPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVIIVLIFYFMGHSY
                     ANEFANFTGLNAYQALVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKKMKMSKD
                     EVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICLYYKLGE
                     TPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLARTLYKNIHKGQYITEDFFEPVA
                     QLIRIAIDLDY"
     gene            complement(2610..>2801)
                     /gene="etgA"
                     /locus_tag="ACHJZ2_005013"
     CDS             complement(2610..>2801)
                     /gene="etgA"
                     /locus_tag="ACHJZ2_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO4570939.1"
                     /translation="INISVAGYILASNIKSRGNTWDAVGAYNAGYFNTPNAVELRRQY
                     AMKIYKTYNKLKNNEQIID"
BASE COUNT          854 a          455 c          504 g          988 t
ORIGIN      
        1 tgtactgggg atattgaaga acgcattagg cattcaacag gtaccaccaa acatggcgct
       61 aacatcagtg tctttgatac tgacaatgtt tattatgtct ccgataatat tacagataaa
      121 tgataatatt tctcaggaac caatcaatta taccgactct gatttttttc aaaaagttga
      181 tgagaaaata ttatcaccat atcgcggttt tttagaaaaa aatacagaga aagacaatgt
      241 agagtttttt gaacgtgcag ctcaaaaaaa attgggtaat gaaactatct taaaaaaaga
      301 ctctctattt atactgttac cggctttcac gatggggcag cttgaagctg cattcaagat
      361 aggttttttg ctctatttac cctttattgc gatagatttg atcatttcca atatcttatt
      421 ggccttgggt atgatgatgg tatcgccagt aacaatttcg attcctttta agatcttatt
      481 attcattctt gttggaggct ggcaaaaatt attcgaattt ctgttggtgg tgaattgatg
      541 gatactggat attttgttca attatgtgtg caaacgttct ggataatatt tatcctctca
      601 ttgcctacag tcatagcggc ctctgttatc ggtattatta ttagtctggt ccaggctata
      661 acgcagttac aggatcaaac attgcctttt ttgctaaaaa taatagcagt gtttgctacg
      721 cttgccctga cttatcactg gatgggaaca acaatcatca acttctcgtc gataatattt
      781 gaaatgattc cgaaggtgaa cggctaatga atgagataat gacggtcata gtatcatcat
      841 tttattgtat actcaggccg ctggggatgt ttattatcct accaatattt tcaacaggtg
      901 ttttactcag taactttatt cgcaactcaa ttatgattgc gtttacgctt ccgatcatag
      961 tagagaacta tacattttcg gaaaaattgc catctggaat ctttcagttg acaggaatag
     1021 cgctaaaaga aataagtatt ggttttttca ttgggttatc atttactatt cttttttggg
     1081 caatagatgc ggctggacag attattgata ctctaagagg ctcaacaata tcttcaattt
     1141 ttaacccgtc cataagtgat tcatcttcta tcactggcgt tatcttgtac caatttatct
     1201 ctgtgatctt tgttattcat ggtgggatac aaagcatcct agataagcta tatttatcct
     1261 acgagatatt accattacaa gccgatattg cattcaatcg tgctttaata gattttttgt
     1321 tttctctatg ggattcattt attaaactga tgttatcatt ttcagttccc atgattatcg
     1381 gtatattctt atgtgatatg gggtttgggt ttcttaacaa aacagcacct cagctaaacg
     1441 tattcacatt atcactgcca gtaaaaagtt tgatagcaat ctttatattg ctattagtta
     1501 ttcatgtttt tcctgatttt attacggcaa atattcattc tgacataatt gatagatcgt
     1561 taccgagcat gattaatgag tgaaaaaaca gaaaagccca cacccaaaaa actaagggat
     1621 ctaaaaaaga agggcgatgt aacaaaaagt gaagaggtaa tggctgcagt gcagtcatta
     1681 atcttatttt catttttttc tttatatggc atgagttttt ttgttgatat agttggatta
     1741 gttaatacga caatagactc gctaaataga ccgtttttgt atgccattcg agaaatatta
     1801 ggtgcggtat taaatatatt tttattatat attttgccaa tttctttgat tgtctttgtt
     1861 ggaactgtta cgactggtgt atcacaaata ggattcatct ttgcggttga aaaaataaaa
     1921 ccatcggctc agaagattag tgtaaaaaat aacctgaaaa atattttttc tgtaaagagc
     1981 atttttgagc tacttaaatc agtatttaag ttagtgataa ttgttctcat tttttatttt
     2041 atggggcatt catatgcaaa tgagtttgct aatttcacag gactgaacgc atatcaagct
     2101 cttgtcgttg ttgccttttt tgtttttctt ttatggaaag gcgtgctatt cggatatcta
     2161 ctcttttcag tatttgattt ctggttccag aagcatgagg gactgaagaa aatgaaaatg
     2221 agtaaagatg aggtgaaacg agaagccaag gatactgatg gtaaccctga aattaaaggg
     2281 gagcgccgtc gccttcattc cgagatacaa agtggaagtt tggctaataa catcaaaaaa
     2341 tcaaccgtta ttgttaaaaa cccgactcac attgcgattt gcctatacta taaacttggg
     2401 gagactccat tacctttagt tattgaaaca ggaaaagatg ccaaagctct acagatcatt
     2461 aaactggctg aactctatga tattccagtg attgaagata ttcctttagc aagaactctc
     2521 tataagaata tacataaagg acaatatata acagaagact tttttgaacc tgtggcacaa
     2581 ttgattcgta ttgcgataga ccttgattat taatcgataa tttgctcatt attctttaat
     2641 ttattatatg ttttatatat tttcattgcg tattgccttc tgagttctac tgcatttggt
     2701 gtattaaagt aacctgcatt atacgcacca acggcatccc atgtattccc tctggatttt
     2761 atgttagagg ctaagatata accagcaaca gatatgttta t
//
LOCUS       DAZFBR010000251         2779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFBR010000251 DAZFBR010000000
VERSION     DAZFBR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.254"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            149..865
                     /gene="nanC"
                     /locus_tag="ACHJZ2_005014"
     CDS             149..865
                     /gene="nanC"
                     /locus_tag="ACHJZ2_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO4570940.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            885..1991
                     /gene="nanM"
                     /locus_tag="ACHJZ2_005015"
     CDS             885..1991
                     /gene="nanM"
                     /locus_tag="ACHJZ2_005015"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO4570941.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2056..>2779
                     /locus_tag="ACHJZ2_005016"
     CDS             2056..>2779
                     /locus_tag="ACHJZ2_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4570942.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEA"
BASE COUNT          830 a          590 c          646 g          713 t
ORIGIN      
        1 acaggagaca ttatacaaaa aatataaaat acagctaccg gttgccaaag acactataag
       61 cctggcaaaa aaatattaca caacataaat gctaattgtt tatgcgggct ttgtattgct
      121 ttctgtatcc tacaaatgag tgaaatttat gaaaaaggct aaaatacttt ctggcgtatt
      181 attactgtgc ttttcgtcac cattaatttc tcaggctgcg acactggacg tacgtggtgg
      241 atatcgtagt ggaagccacg cctatgagac tcgactcaaa gtcagtgagg gatggcaaaa
      301 tggatggtgg gcaagcatgg aaagtaatac ctggaatacc attcatgata ataaaaagga
      361 aaatgccgca ctcaatgatg ttcaggttga agttaattac gcgattaaac ttgatgatca
      421 atggacggtg cgcccgggaa tgttaacgca ttttagcagc aacggcacac gctacggacc
      481 ctacgtaaaa ctgtcctggg acgcgacaaa agatcttaat tttggcattc gctatcgtta
      541 cgactggaaa gcttaccgac aacaagactt atccggtgat atgtctcgtg ataacgttca
      601 tcgttgggat ggatatgtca cttaccatat taatagtgat ttcaccttcg catggcaaac
      661 gacgctatac agcaaacaga acgattatcg ctatgcaaac cataagaaat gggcgacgga
      721 aaatgcattt gttctacaat accatatgac gcccgatatt acgccataca tagaatatga
      781 ctacctggac cgtcagggtg tttacaacgg cagagataat ttatcggaaa acagttatcg
      841 cattggtgtg tcatttaaac tgtagtagac aggagacagt cacaatgaat aaaacaataa
      901 cggcgcttgc tatcctaatg gcttcatttg ccgcaaacgc gtctgtatta ccggaaactc
      961 ctgtaccatt taaaagtggt accggagcaa ttgataacga cactgtctac attggtttag
     1021 gtagcgcagg tacggcatgg tacaagctgg agacacaggc caaagataaa aaatggacag
     1081 cgttagctgc attccctggt ggaccaagag atcaagcaac ctcggcattt attgatggca
     1141 atctgtatgt gtttggcggc attggcaaaa acagcgaggg cttgactcag gtatttaatg
     1201 acgtacacaa atacaacccc aaaaccaata gctgggttaa attgatatcg cacgcgccga
     1261 tgggcatggc gggccatgtg acttttgtac acaacggcaa ggcttatgtt actggcggtg
     1321 ttaaccagaa tatcttcaat ggctattttg aagatctcaa cgaagctgga aaagattcaa
     1381 ccgctgtaga taaaatcaat gcacactatt ttgacaaaaa agcagaagat tatttcttta
     1441 ataagtttct gttgtctttt gatccctcta cacagcaatg gagttacgct ggcgaatctc
     1501 cctggtacgg gacggctggt gcggcggttg tgaataaagg tgataaaacc tggcttatta
     1561 atggcgaagc caaacccgga ttgcgaacgg atgccgtatt tgaacttgat ttcaccggta
     1621 ataatttaaa atggaatagg cttgctcccg tctcatcacc agatggcgtc gctggcggtt
     1681 ttgcggggat aagcaatgat tctcttatat ttgccggagg ggccggattc aaaggttcac
     1741 gagaaaatta ccaaaacggt aagaactatg cgcatgaagg cctgaaaaaa tcatatagca
     1801 ctgatattca tctttggcat aacgggaaat gggataaatc gggtgaatta tcgcaaggtc
     1861 gggcctacgg agtatcattg ccctggaata atagtctatt gattattggc ggtgaaactg
     1921 caggcggcaa agcggtgacg gattcagttt tgatctctgt gaaggataat aaagtcacag
     1981 tacaaaacta acgcttcagg gccccggtaa aggggccctg ctatcaattt gtcatttcaa
     2041 ttacgcgaaa tttatatgaa cgcaataata tcgcctgatt attactatgt tcttactgtt
     2101 gctggtcagt ctaatgccat ggcgtatggc gaaggactgc cattaccgga cagggaagat
     2161 gcgcctcatc ccagaattaa acaattagcg agatttgcgc atacgcatcc cggaggcccg
     2221 tcatgtcact ttaacgacat tattccactg actcactgcc cacacgatgt tcaggatatg
     2281 cagagttatc accatcctct ggcaacgaat catcaaacac agtacggcac cgttggccag
     2341 gcactgcata ttgcacggaa attactgccc ttcattcctg ataatgcagg gattctcatc
     2401 gttccgtgtt gccgtggcgg atcggctttt accgcaggca gcgaggggac atattcagaa
     2461 cggcacggag ccagccatga tgcttgtcgt tggggaacgg atactccgct ataccaggat
     2521 ttagtcagca gaacgcgagc cgcactggta aaaaatccgc agaacaaatt cctcggcgta
     2581 tgctggatgc aaggcgaatt tgacttaatg accagtgact acgcgtcaca tcctcaacac
     2641 tttaatcata tggttgaagc ctttcgtagg gatctaaaac aataccattc tcagcttaat
     2701 aatattactg acgcgccgtg gttttgcggc gataccacct ggtactggaa agaaaatttc
     2761 cctcatgcgt atgaagcta
//
LOCUS       DAZFBR010000252         2777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFBR010000252 DAZFBR010000000
VERSION     DAZFBR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.255"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..546
                     /locus_tag="ACHJZ2_005017"
     CDS             <1..546
                     /locus_tag="ACHJZ2_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4570943.1"
                     /translation="KTGAEAIRALAMQIPAFRQKLSDGWYLVRIAGRDTGENELSARL
                     NEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIAVAWWNPVGWLGAAAVSGMYAAGA
                     SMILGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRV
                     VSQEISTADEGDGGQVVVIGR"
     gene            607..2777
                     /locus_tag="ACHJZ2_005018"
                     /pseudo
     CDS             607..2777
                     /locus_tag="ACHJZ2_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
BASE COUNT          603 a          712 c          933 g          529 t
ORIGIN      
        1 aaaacggggg ccgaagccat ccgggcgctg gccatgcaga tcccggcgtt tcgtcagaag
       61 ctgagcgacg gctggtatct ggtacggatt gccgggcgtg atacaggtga aaatgaatta
      121 tctgcccgtc ttaatgagcc gctggcaaat ggtgccgtga tccacatcgt gccgcgcctg
      181 gcgggtgcta aaagtggcgg tgtgtttcag gcagtgctgg gtgcggcgct gattgcggtg
      241 gcatggtgga accctgtggg ctggctgggg gccgcggctg tatcgggtat gtatgcagca
      301 ggggccagta tgatcctggg tggtgtggcg cagatgctgg caccgaaagc cagaactccc
      361 cgtacacaga caacggataa cggcaaacaa aacacctatt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gcccgttctg tacggtgaaa tgcgcgtggg atcacgtgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttgtcgt ttatggagca
      601 tgacgaatgg gtaaaggaag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aaggaccgat tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaaggtaat
      781 accaacatct ccggtgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttctgagacg gtgctgggta cggaagtgaa atacgacacg
      901 ccgatcaccc gcaccatcac gtcggcaaac atcgatcgtc tgcgctttac tttcggtgtg
      961 caggcactgc gggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     1021 gttcagatac agcgtaatgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     1081 accacgtcgc agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgtttaat
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat tatcgatgtg aaacagtgct acccgaacac ggcactggtt
     1261 ggcgtgcagg tggactcgga gcagtttggc agtcagcagg tgagccgtaa ttatcatctt
     1321 cgcgggcgca ttctgcaggt gccgtcaaac tatgatccgg aaaaacgcac ttacagcggc
     1381 atctgggacg gaacgttaaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc acccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag taccggacgg ctttggcggc
     1561 acggagccgc gcatcacctg taatgcgtac ctgaccacgc agcgcaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accggccgtc ggataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc tttagcgccc tgaaagaccg ccataatgcc
     1801 gttgaggtga actggactga cccggacaac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattgcccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagtcggg ggcaggcaca ccgtgccggg ctgtggctga ttaaaacgga actgctggaa
     1981 acgcagaccg tggacttcag cgtgggcgca gaagggcttc gccatgtacc gggcgatgtc
     2041 attgaaatct gtgatgatga ctatgcgggg atcagcatcg gtgggcgtgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggcacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt tcagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgg gttcctgacg gtgttgctga atacagcgtg
     2281 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgacgacg gcacgtatgc catcactgcc gtgcagcatg tgccggagaa agagggcatc
     2401 gtggataacg gggcgcactt tgacggtgac cagagcagca cggtgaatgg tgtcacgccg
     2461 ccagcggtgt agcacctgac cgccgaagtc tccgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacgacgga aaccacatac
     2641 cgcttcacgc agctggcgct ggggaactac aggctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttccggattg ccgcaccggc agcgccgtca
     2761 cagattgagc tgacacc
//
LOCUS       DAZFBR010000253         2715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFBR010000253 DAZFBR010000000
VERSION     DAZFBR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.256"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..185
                     /locus_tag="ACHJZ2_005019"
     CDS             <1..185
                     /locus_tag="ACHJZ2_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4570944.1"
                     /translation="TPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTMLIRELSGRGR"
     gene            182..532
                     /gene="tnpB"
                     /locus_tag="ACHJZ2_005020"
     CDS             182..532
                     /gene="tnpB"
                     /locus_tag="ACHJZ2_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4570945.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            563..2148
                     /locus_tag="ACHJZ2_005021"
                     /pseudo
     CDS             563..2148
                     /locus_tag="ACHJZ2_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2177..2320
                     /locus_tag="ACHJZ2_005022"
                     /pseudo
     CDS             2177..2320
                     /locus_tag="ACHJZ2_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2431..>2715
                     /locus_tag="ACHJZ2_005023"
     CDS             2431..>2715
                     /locus_tag="ACHJZ2_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4570946.1"
                     /translation="MISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEH"
BASE COUNT          707 a          691 c          756 g          561 t
ORIGIN      
        1 taacgccgga tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca
       61 ctcagaccag agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacatgacgc
      121 tggaaaatcc atcgccagag ctgctcacga tgttaatccg cgaactgagc gggaggggaa
      181 gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgtg
      241 taaatccttc aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc
      301 cggtcacctg tttatcttcc gtggccgacg gggggacacc gttaaaattc tttgggctga
      361 tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa ggccagttta tctggcctgc
      421 ggtgcgtgac ggtaaggtat ccattacccg ctcgcaactg gcaatgctcc tcgataagct
      481 ggactggcgt cagccaaaaa catcccgcct taacgccctg acaatgttgt aaaaaacacc
      541 tggccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcattg cagaactgga
      601 aagccagctc cgtcagaaag accagcaact gagtctggtt gaagagacgg aggccttcct
      661 gcactggcac gtgccgaaga aaagatcgaa gaagatgaac gggaaataga acatctgcgg
      721 gctcagatag aaaaactgcg ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt
      781 gaagttgaac aggctgaggc cctgctgaaa caacgcgaac aggacagcga tcgttacagt
      841 gggcgggaag acgatccaca ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg
      901 ttaccggcac accttccccg tgaaatacag cgtctggaat ctgaagaaag ctgttgcccg
      961 gagtgtggcg gtgagctgga ttatctgggg gaagtcagtg cagaacaact ggaactggtg
     1021 agcagcgccc tgaaagtgat ccgcacagaa cgggtaaaaa aagcgtgtac aaaatgtgac
     1081 tgcatcgttg aagcaccggc gccgtcccgc ccgatagagc gtggtatcgc gggccccgga
     1141 ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt
     1201 gaaatctttg cccgacaggg tgtcgaactg agccgtgcat tactctccaa ctgggttgac
     1261 gcgtgctgcc agttaatgac gccgctgaat gatgccctgt accgttatgt gatgaatacc
     1321 cgcaagcttc acactgacga cacaccggta aaggtactgg caccgggcct gaaaaagacg
     1381 aaaacagggc gcatctggac gtatgtccgg gatgatcgca atgcgggttc gtcatctcct
     1441 ccggcggtct ggttcgcgta ctcaccgaac cggcagggga aacacccgga gcaacacctc
     1501 cgccccttcc ggggtatcct gcaggcggat gcgttcacag gttatgacag gctgttcagt
     1561 gcagaacgtg aaggtggtgc gctgacagaa gttgcgtgcc gggcccatgc ccggagaaaa
     1621 atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc
     1681 agtgaactgt acgccatcga ggatgaaata cggggattac cagagtcaga gcgtcttgca
     1741 gtcagacagc agcgaagcaa agcgttactg acgtcgctgc atgaatggat gatggagaag
     1801 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1861 tgggacgccc tctgttatta cagtgatgac ggactggcgg agacggacaa taacacagcg
     1921 gaaagagcgc ttcgtgcagt ctgtcttgga aagaaaaatt acgtgttctt cggtagcgat
     1981 cacggcggcg agcgtggtgc actgctatac gggctgatcg gcacctgccg tctgaacggt
     2041 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccctccaac
     2101 cgggttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     2161 tgctccgttg acgcttacct tctttcattc gctgaaggtg gaatgtatcc acggggaacg
     2221 ctttagcagc cgggaaataa tgcgggaaac ggtgtttaat tatatcgagt gtgattacaa
     2281 tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg gactgtcttg cactgcaaat
     2341 attcagaaat gagtctatgc agttcaccag taaaatcagc catcagagag ggaatgtagt
     2401 ggcacagtaa atttggccac ctgattaaag gtgatatcct caccacaaca caaaacaggt
     2461 gacttaatga acaagaaaac caaacgtact ttcacccctg aattcaggct ggaatgtgca
     2521 cagctaattg ttgataaggg ctactcatat cgacaagcca gtgaagcgat gaatgtcggt
     2581 tctaccacgc ttgagagttg ggtgcgccag ctcaggcgag agcgtcaggg gattgcgccc
     2641 tctgccacac ctattactcc agaccagcaa cgtatccgcg aactggaaaa gcaggttcgc
     2701 cgcctggagg aacac
//
LOCUS       DAZFBR010000254         2714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFBR010000254 DAZFBR010000000
VERSION     DAZFBR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.257"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..923
                     /locus_tag="ACHJZ2_005024"
     CDS             <1..923
                     /locus_tag="ACHJZ2_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4570947.1"
                     /translation="LEKVDLTEDNASRLDEFSKEWKDANDKWNAMWGVKIEQTKDGKH
                     YVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVFLKRL
                     TAPTITSGGNPPVFSLTSDGKLTAKNADISGSVNANSGTLNNVTVNENCTIKGMLEAT
                     QVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYS
                     DPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFK
                     VFHKGNQRAGNITDCTVIVTKKAASGISIR"
     gene            994..1593
                     /locus_tag="ACHJZ2_005025"
     CDS             994..1593
                     /locus_tag="ACHJZ2_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4570948.1"
                     /translation="MRKVCAAILSAAICLAVSGVPAWASEHQSTLSAGYLHASTDAPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDYVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYFRVTDNKRKTHDVLTGSDDARYSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            1653..>2714
                     /locus_tag="ACHJZ2_005026"
     CDS             1653..>2714
                     /locus_tag="ACHJZ2_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4570949.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTISVYEDSQPGTLNDFLGAMTEDDARPE
                     ALRRFEQMVEEAARHAEEAKKNAGEAETSARNAGISASKAEASAANADTSAEDASESA
                     RQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGNAARDATT
                     ATEKARESAESAQSAEQSRIAAEDAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERG
                     DTGPAGATGERGPGGDTGPAGPQGPKGDRGERGETGLTGSTGPQGPKGDTGATGPAGP
                     QGPKGETGAAGPVGATGPQG"
BASE COUNT          722 a          627 c          847 g          518 t
ORIGIN      
        1 tgctggaaaa agtcgatctg acggaggata acgccagcag actggatgag ttttcgaaag
       61 agtggaagga cgctaacgat aaatggaatg ccatgtgggg cgtcaaaatt gagcagacca
      121 aagacggcaa acattatgtc gcgggtattg gcctcagcat ggaggacacg gaggaaggca
      181 agctgagcca gtttctggtt gccgccaatc gtatcgcgtt tattgacccg gcaaacggga
      241 atgaaacgcc gatgtttgtg gcgcagggca accagatatt catgaacgac gtgttcctga
      301 agcgcctgac ggcccccacc attaccagcg gtggaaatcc accggtattt tccctgacat
      361 cagacggaaa gctgaccgct aaaaatgcgg atatcagtgg cagtgtgaat gcgaactccg
      421 ggacgctcaa caacgtcacg gtaaatgaaa actgtacgat taagggcatg ctggaggcga
      481 ctcaggtcag aggtgacttc gttaaagctg tatccaaatc atttccgaaa caggctggta
      541 cgtggggtaa cacggaaaca ccaaacggga cggttacagt caccatcagc gatgatcata
      601 actttgaccg tcaaatcatt attccgccca ttatctttaa cggaatagcg tatagcgatc
      661 cgggaagtgg taataacccg ggaggtacaa gatacacggg ttatggtttt gaagttcgca
      721 aaaacggtgt attaatcgca tccagagaaa ctaaaggggc cattcccggt agctacagtg
      781 cggttattga tatgccgagt ggcaggggaa gcgtcactct ggagtttaag gttttccata
      841 aaggcaatca gcgggcaggt aatatcaccg actgtacggt gattgtgacc aaaaaagcgg
      901 cttccggcat cagtatccgt tgaaatattt ataaccctaa taacgggcgc cagaaatggc
      961 gcctttttta ttgcagaaaa tcgggaggta attatgcgta aagtttgtgc agcaattttg
     1021 tccgcagcca tttgtctggc cgtatccggt gtgcctgcat gggcgtctga gcatcagtcc
     1081 acgctgagcg caggatatct tcatgccagt acggacgctc ccggcagcga tgatctgaac
     1141 gggattaacg tgaaataccg ttatgagttt acggacacac tggggctgat tacgtccttc
     1201 agttatgcca acgccgaaga tgagcaaaaa acgcattaca gcgatacccg ctggcatgag
     1261 gattacgtgc gtaaccgctg gttcagcgtg atggcagggc cgtctgtgcg tgtgaatgaa
     1321 tggttcagtg cgtatgcgat ggcgggtgtg gcttacagcc gtgtgtcgac tttttccggg
     1381 gattatttcc gcgtaactga caacaagagg aaaacgcacg acgtgctgac cggaagtgat
     1441 gacgctcgct acagcaacac atctctggcg tggggggctg gcgtgcagtt taacccgacc
     1501 gaatcagtgg ccgttgatgt cgcttatgaa ggctccggca gcggtgactg gcgcactgac
     1561 ggtttcatcg tgggtgtcgg ttataaattt tgattagtaa cacagcgtta tgacagcccg
     1621 ccggttcagg cgggcttttt tgtggagtgg gtatggcagc agtacaaata tcaggcgtgc
     1681 tgaaagatgg tgcgggaaaa ccaatacaga actgcaccat tcaactgaaa gccagacgta
     1741 acagcaccac ggtggtggtg aacacggtgg cctctgaaaa tccggatgag gcagggcgtt
     1801 acagcatgga cgtcgagtat ggtcagtaca gcgtcactct gttggtggag ggattcccgc
     1861 catcacatgc cgggaccatc tccgtgtatg aagattctca accgggtacg ctgaatgatt
     1921 ttctcggtgc catgacggag gatgatgccc gtccggaggc actgcgtcgt tttgaacaga
     1981 tggtggaaga agcagcgcgt cacgcagagg aggcgaagaa gaatgccgga gaagcagaga
     2041 cgtccgcgag gaatgccggc atatcagcca gtaaggcgga agcgagcgcc gcaaatgctg
     2101 atacttcagc agaggatgca tcggagtcag cccggcaggc ggcagaaagt gcagcctctg
     2161 caaagaagtc agaggaagcg tcctcgtcct cagcctctga ggccgctcaa aaagccagtg
     2221 agtcattaca aagtgcaaca gatgccgagt tgtcaaaaaa gacggcagaa agtgcagccg
     2281 gtaatgcagc cagggatgca acgaccgcaa cagaaaaagc ccgggaatca gcagaaagcg
     2341 cacagtcagc ggaacaaagc agaatagcgg cggaagacgc cgtaaacaga attcccaccg
     2401 tggtggggcc tcccggacca aagggggaac cgggtcccgc gggtcctcag gggccgaagg
     2461 gagataaagg agagcgtgga gacaccggtc cggcaggggc aaccggtgaa cggggaccgg
     2521 gaggagatac aggtccggca ggtccgcagg ggccgaaagg cgacagggga gagcggggag
     2581 agaccggtct gacaggaagt acaggtccac aggggccaaa gggagatacc ggggcaacag
     2641 gtccggcagg accgcaggga ccgaaagggg aaacaggtgc ggctggcccg gtgggggcta
     2701 ccggacctca gggg
//
LOCUS       DAZFBR010000255         2653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFBR010000255 DAZFBR010000000
VERSION     DAZFBR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.258"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(1..1435)
                     /locus_tag="ACHJZ2_005027"
                     /pseudo
     CDS             complement(1..1435)
                     /locus_tag="ACHJZ2_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1381..1602)
                     /locus_tag="ACHJZ2_005028"
     CDS             complement(1381..1602)
                     /locus_tag="ACHJZ2_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570950.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGDPEKTRNQDVM"
     gene            complement(1647..>2653)
                     /locus_tag="ACHJZ2_005029"
     CDS             complement(1647..>2653)
                     /locus_tag="ACHJZ2_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO4570951.1"
                     /translation="ALEVARRQYPDDSRLHHVLKSAVGAGTTTDPQWAGSLSEYQEYA
                     QDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQVSGGAAGWVGEGKAKPLTKFDF
                     ESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAAVAD
                     VSPASITHDVKGTASSGNPDADAEAAFGQFVTANLQPTGAVWLMSSTNALALSMRKNA
                     LGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREASLE
                     MQSEPTSDSSTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVITGVNYGTASGG
                     "
BASE COUNT          545 a          866 c          664 g          578 t
ORIGIN      
        1 cgctgtaaat ccgccagcac catggcgaat tcttcgccca gtgcacgaat aatggacagt
       61 tcccgttccg tcattttcgc agtatccccc tgaacatcgc tttcaccgca tcatgctctg
      121 tttcactgat tgccttatta ccgtcagatg cgccatcagg cagttgtgct gagactgttt
      181 tcccggacga cgcgaacgga tcctcacggg catcacgacg ggacagcgcc tccagactgt
      241 agttctgctg ctgaagatac agtgcatcac cgccggccag gggcggcagg ttctcacgtt
      301 tacgggcctc attgggcgtc agcagcgtat ttttcaccga ctcacccagc gttttcatgc
      361 gccgttcgct gtccattctc agcagcgtgg tgacgtcaaa ctccgtgctc tcgttttccc
      421 ccgtttccag cgcctcatcc agtaacagct caatggactc aatcagcgtc tgcagacact
      481 gggaataata ctgctgctcc agcgcctcca cgttatcact ggagggaggt tgtcccacac
      541 caatcttgta ggccgggaca cggaacaccg aacagacaat ttcagcggtc attttcagtt
      601 gttccaccgt ctgcgcatcc accggtgaaa acgtcgtggg gttatatttc gccccgttgc
      661 tcagaatcgc cgttttcccc gcattttcgc cggtataccc gctgtcccag ttgctcttca
      721 gttttttcgc attttcttcc gttatactgc cggggatctc aatcaccccg gacggcctgc
      781 cgccatttct gaaaaaagac gttgaatttg cctgaatatg atacccctgc gtggcagcca
      841 gcccggcagc atacaccggc ggcagcccca caagcggatg aaaaaaacag ttaaaccggt
      901 cgtggatcac ttcccgggca ggcaccgtca ccgcctccgt gatcccgcag ttccggtccg
      961 gcgtgatgcg atagaacacc tcgccgtcat ccgccaccag aggttcaacc cggctccagt
     1021 ccagaatacg cagttctttg atctgcccac gggagttacg gattttcagc accaccgtat
     1081 tgccgtgacg cagtttggcg ttcagccaca gttcaaaaaa ctggatacga ttctgctgtg
     1141 cattgggacg acgacagaga cgggcaatat ccccctgccg tttttcacgg cggatcccct
     1201 gtgtatcggt ctgcatcagg cgcagccgca ttttggcgat atcctgggat atcagcgaaa
     1261 tgcaagaaaa caccgcatga aaggagagga cactttccgg atcggctttc actccctgct
     1321 gccaggcacc ggcaaaaggc tcagccaccg cctgaaacag gctggtccag cccacctctt
     1381 ttacatcacg tcctgatttc tggttttttc ggggtcgccg caaaaggttc cacattcgcc
     1441 atgctccgca tcacgtttct ttttctgacc tgccggacgt cgcgctgtga tgtactccgc
     1501 cttccccagg cgaaccagca cctccgcaca cggctgtgcc acatcacgga tatccccggc
     1561 ccgggcatca tgcgtgccct gcagatactg gatttttgcc atcagttact gcgggaagct
     1621 agcgcctccc gccctcctca tcagactcag ccgccggacg cagttccgta gttcacaccg
     1681 gtgatcacag ccaccgccgc ggtacggcga cgacgccagt tgatccagcg ctccgcacgg
     1741 atggccacgc tgccggtctg gaacatggag accagctcca ccggtgacgg tgtgctgctg
     1801 tcgctggtcg gttcagactg catctccagt gacgcttcac gggacatatc caccgccaca
     1861 ccaccgtcat ccgccagata aatatccggc gcattcacca gtaccagctg gtcacccaca
     1921 tactgggaga caatcaccgg cagcccctgg aaggtcccgc ccagcagggt catgtccgga
     1981 tattccttct gccccagcgc atttttacgc atggacagcg ccagggcatt cgtgctggac
     2041 atcagccaga ccgcaccggt gggctgcaga tttgccgtca caaactggcc aaacgcggcc
     2101 tcggcatccg catccggatt accgcttgac gccgtgccct tcacatcatg ggtgatggac
     2161 gcaggggaga catctgccac cgccgctttt ttcgggtcca caaagtctgt atccagacgc
     2221 gccaccaccg cttccgccag cgcattacgg accagtgcat cagcagccgg actggaaaaa
     2281 cggatcaatt cttccgtcag taccgcaatg gccgacacct tcgcatgact gaaggtgatg
     2341 gattcaaaat caaacttcgt caggggtttt gccttaccct cccccaccca gccggcagct
     2401 ccgccggaca cctgggcatg cacacggata ttgaacggca cctgacgaag tgcagggatc
     2461 ccgccctgac caaatcgccc gataatggtc tgaggacgca ggtaatcaat aaagtcctgt
     2521 gcgtattcct gatattcaga caggctgcct gcccactgcg gatccgtggt ggtccccgcg
     2581 cccactgccg atttcaggac atgatgcaga cgactgtcat ccggatactg acgacgggcc
     2641 acttccaggg ctt
//
LOCUS       DAZFBR010000256         2596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFBR010000256 DAZFBR010000000
VERSION     DAZFBR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.259"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(1..642)
                     /locus_tag="ACHJZ2_005030"
                     /pseudo
     CDS             complement(1..642)
                     /locus_tag="ACHJZ2_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            630..1046
                     /locus_tag="ACHJZ2_005031"
                     /pseudo
     CDS             630..1046
                     /locus_tag="ACHJZ2_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            1174..1710
                     /gene="yfdR"
                     /locus_tag="ACHJZ2_005032"
     CDS             1174..1710
                     /gene="yfdR"
                     /locus_tag="ACHJZ2_005032"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4570952.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELHKCA"
     gene            1701..2051
                     /locus_tag="ACHJZ2_005033"
     CDS             1701..2051
                     /locus_tag="ACHJZ2_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HFO4570953.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            2048..2521
                     /locus_tag="ACHJZ2_005034"
                     /pseudo
     CDS             2048..2521
                     /locus_tag="ACHJZ2_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
BASE COUNT          694 a          585 c          637 g          680 t
ORIGIN      
        1 atttaccagc catgctgaca ccgaaaactc cagcgtcatg tgaccgtgac cggcaggcat
       61 atcaataacg ccactgtaaa tcagcgtatt atccagcgcg gtacggttat aaatttcagc
      121 accgtttttc cgcactatca gacggcatga ggagtaaata tcagtatgct ctctctcatg
      181 cttagcgcca ctgaatgcca ccgccggaat aacaatctgc cggtcaaacg gctgatcgtc
      241 ataaaccctg acggtaatgg tccctgatgg ccaccgttcc ggtgcacggg agtcccgggg
      301 gaaagctttg cccactgttt taacgagatc gccttcaatc tggttcgcgg acagttttcc
      361 cagaacccga cagttctcgt taatcgtgac gttgttgagc gtcccggagt tcgcattcac
      421 gttaccactg atatccgcat ttttagcagt cagctttcca tccggtgtca gggaaaatgc
      481 cggtggattt ccaccactgg taatggtggg ggccgtcagg cgcttcagga acacgtcgtt
      541 catgaatatc tggttgccct gcgccacaaa catcggcgtt tcattcccgt ttgccgggtc
      601 aataaacgcg atacgattgg cggcaaccag aatggattga agactgggcc gactaccttg
      661 tgggctttga tgctaatggt gacaccattc aggcaacaaa agcggctgcg gcggtccgta
      721 aaatcacgat tgaagcaaac cagaccgctg attttgaaga taatgacttc agcggcaaac
      781 gctccctgat ggagtctgtc gaagcgaaga ccaaagacat tatgccagtg gcatttgaat
      841 ttaaatgcat tccgtttgaa ggtctgaaag aacgtccgtt taaattacgc ctcagcatta
      901 tcactggcga tcgtccggta ctggttctgc gcattattca gctggaggcg gtgcaggaag
      961 aaatggctaa cgaatttcgt gatctgcttg ttgagaaatt caaggacagc aaagtagaaa
     1021 cctttattgg tactttcacc gcctgatttc attactgcaa atgcccctga gggggcattt
     1081 atggaaacgt aatttactca ataatcgccg gatggtgagg gattcttttt accagaattc
     1141 agcgcggtgc agcgcatata cgtggagaac aaaatgtcat ttattaaaac tttttccggg
     1201 aagcattttt attatgacag gataaataaa gacgacatcg atattaacga tatcgcggtt
     1261 tccctttcaa atatctgtcg ctttgccggt catctttcgc acttctacag cgtcgcccaa
     1321 catgcggttc tttgcagcca gctggtaccg caggaatttg cttttgaagc attaatgcat
     1381 gatgcaacag aagcgtattg ccaggacatc cccgctccac tgaaacgcct tcttcctgac
     1441 tataaacgga tggaagaaaa aattgacgcc gtaatccgtg agaaatacgg gttaccccca
     1501 gttatgagta cgcccgtgaa atatgccgat cttatcatgc tggcaaccga acgccgcgat
     1561 ctcgggcttg atgatggctc tttctggcct gtactggaag gcatcccggc aacagagatg
     1621 ttcaacgtga ttccactggc acctagccat gcctacggga tgtttatgga acgttttaac
     1681 gagttatcgg agttacacaa atgcgcatga atgttttcga aatggaaggg tttcttcgcg
     1741 ggaaatgtgt accgcgagat ctgaaagtga atgaaacaaa tgctgagtat ctggtgcgta
     1801 aattcgatga agtacgtgct gaggctcgca acgagggtat taactatacc gcaagccgtc
     1861 ttgctgctgc tttcaatcac ggatttatca ataagccttt ggctgaagtt ttcgacgtta
     1921 cacgcatgat tctgtcagca aaagaagagt tagctaatga atcgcatccg attgatggcc
     1981 tgtccggtga atatgcagag aaatcccttg aagaatgggc ggaacggctt cgcaaaggag
     2041 gcagccagtg actggacatg cagcaatcct cgacatgtgc tgtggcagtc gcatgttctg
     2101 gttcgataag aatgacgacc gggcgatatt tagcgatatc agaaaggaag agcacacatt
     2161 gtgtgatgga cgacgcctga ttatcagtcc tgatctgata gctgattttc gtgcactacc
     2221 atttgcagac gcatcttttt cgatggttat attcgaccct ccgcatcttg agcgtgttgg
     2281 tgataacgcc tggatgggaa agaaatatgg acggctgaat aaagatacct agcgtgatga
     2341 tttgcggcag ggatttaaag aagcctttcg tgtgttgcgt ccatccggcg ttctgatttt
     2401 taaatggaat gaaacgcaaa tacctgttcg ccagatattg gtactgaccg acagaaaacc
     2461 tgttatcggt caacgaacag gaaaaaacga taaaacccac tggattattt ttatgaaata
     2521 ggcatccagt gagtaggttc gtaaggttac agatacgtat atctgaataa ttaaattcag
     2581 ttctgtaaat aaaatt
//
LOCUS       DAZFBR010000257         2581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFBR010000257 DAZFBR010000000
VERSION     DAZFBR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.260"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            49..2196
                     /locus_tag="ACHJZ2_005035"
     CDS             49..2196
                     /locus_tag="ACHJZ2_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO4570954.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIV
                     NFITKKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSY
                     YYYDNIDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIG
                     LKHTLVMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRIN
                     LALNNLTDKRYRTAHETIPAAGFNAAIGFVWNF"
BASE COUNT          759 a          567 c          574 g          681 t
ORIGIN      
        1 aattatcatt acagcaatca tttgtacttt gtattaatga gggatgaaat gttatataat
       61 ataccttgtc gaatttatat cctttccact ctgtcattat gcatttctgg gatagtttct
      121 actgcaaccg caacttcttc agaaacaaaa atcagcaacg aagagacgct cgtcgtgacc
      181 acgaatcgtt cggcaagcaa cctttgggaa agcccggcga ctatacaggt tattgaccaa
      241 caaacattgc agaactccac caatgcctcc atagccgata atttgcagga catccccgga
      301 gtagagataa cagacaactc cttggcaggc cgtaaacaaa tccgcattcg tggcgaagca
      361 tcctcccgtg ttttaattct cattgatggt caggaggtaa cttatcagcg cgccggagat
      421 aattatggtg tgggactgtt gatagatgag tctgcgctgg agcgtgttga ggtagtgaaa
      481 ggtccatatt ccgtactgta cggttcacag gcaattggcg gtattgttaa cttcatcacc
      541 aaaaagggag gtgacaaact tgcatctgga gttgtgaaag ctgtttataa ttccgcaaca
      601 gcaggctggg aagaatcaat cgcggtccag gggagcatcg gtggatttga ttatcgcatc
      661 aacggtagtt attctgatca gggcaatcgt gatacgccgg atggacgtct gccgaatacc
      721 aactatcgta acaatagtca gggtgtatgg ttgggttata actccggaaa ccatcgtttt
      781 ggcctctcgc ttgatcgcta cagactcgcg acacaaactt actatgagga tccagacgga
      841 agttatgagg catttagtgt caaaatacct aaacttgaac gagagaaagt tggggtattc
      901 tatgacacag acgtggacgg tgactatcta aaaaaaattc atttcgacgc gtatgagcag
      961 accatccagc gccaatttgc caacgaagta aaaacgacac agcctgttcc cagtccgatg
     1021 attcaggctc tgaccgttca taacaagact gacacccatg ataagcaata cactcaggcg
     1081 gtcacattgc agagtcactt ttcgctgcct gctaataatg aacttgttac cggtgcacag
     1141 tacaaacaag acagggtcag ccaaaggtcc ggtggcatga cctcaagcaa atctctgacc
     1201 ggcttcatta ataaggaaac acgaactcgc tcctattatg agtcagagca aagtacagtc
     1261 tcactattcg cacaaaatga ctggcaattc gccgatcact ggacatggac aatgggagtt
     1321 cgccaatact ggctttcttc aaagttgacg cgtggtgacg gagtatcata taccgcaggc
     1381 attataagcg atacctctct tgccagagag tctgcgagtg atcacgaaat ggtaacatct
     1441 acaagcctgc gctattcagg tttcgataac ctggagttac gcgctgcgtt cgcgcaaggc
     1501 tacgtatttc ccacactctc ccagcttttt atgcagacat ctgcgggcgg cagtgtcaca
     1561 tacggaaatc ctgatcttaa ggctgaacac tccaataact ttgaattagg tgcacgatat
     1621 aatggtaatc agtggctgat tgacagcgca gtttactact cagaagctaa agattatatt
     1681 gcaagtctga tctgtgatgg cagtatagtt tgcaatggta acaccaactc ctcccgtagt
     1741 agctactatt attatgacaa tattgatcgg gcaaaaacat ggggactgga aataagcgcg
     1801 gaatataatg gctgggtttt ctcgccatat atcagtggca atttaattcg tcggcaatat
     1861 gaaacttcaa cattaaaaac aactaataca ggtgaaccag cgataaacgg acgtataggg
     1921 ctgaaacata ctcttgtgat gggtcaggcc aacataatct ctgatgtttt tattcgtgct
     1981 gcctctagtg caaaagatga cagtaacggt accgaaacaa atgttccggg ctgggccact
     2041 ctcaactttg cagtaaatac agaattcggt aacgaggatc agtaccggat taacctggca
     2101 ctcaataacc tgacagacaa acgctaccgt acagcacatg aaactattcc tgcagcaggt
     2161 tttaatgcag ctataggttt tgtatggaat ttctgatgac aaaaacaatc gttatggaat
     2221 tctttgtcct gatctgcccg ctttgcgggc aggctaacga acgtattgtg tttgttgaag
     2281 gtttttcgac gttgttttcc atctgtgggt gaatatttat tcttcccgcc agcatgactc
     2341 tatactcatt gctacgacaa tgacttataa agctggttac tgaacctata cctttgtaat
     2401 tcaaaagtga tatcagttca caacgcaata ctatttttag attatttact ctcacccaga
     2461 tactgaatca atggataata ccttttgatt tatcccgacg cggaagaagg tggtgaaccg
     2521 ccccggaaat cctggagact aaactccctg agaaagaggt aaacaggatg actaaaaata
     2581 c
//
LOCUS       DAZFBR010000258         2564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFBR010000258 DAZFBR010000000
VERSION     DAZFBR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.261"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..449)
                     /locus_tag="ACHJZ2_005036"
     CDS             complement(<1..449)
                     /locus_tag="ACHJZ2_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4570955.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDV"
     gene            complement(497..706)
                     /locus_tag="ACHJZ2_005037"
     CDS             complement(497..706)
                     /locus_tag="ACHJZ2_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO4570956.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(802..1176)
                     /locus_tag="ACHJZ2_005038"
     CDS             complement(802..1176)
                     /locus_tag="ACHJZ2_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4570957.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(1191..1907)
                     /locus_tag="ACHJZ2_005039"
     CDS             complement(1191..1907)
                     /locus_tag="ACHJZ2_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO4570958.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(1973..2317)
                     /locus_tag="ACHJZ2_005040"
     CDS             complement(1973..2317)
                     /locus_tag="ACHJZ2_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO4570959.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(2314..>2564)
                     /locus_tag="ACHJZ2_005041"
     CDS             complement(2314..>2564)
                     /locus_tag="ACHJZ2_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO4570960.1"
                     /translation="SRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFYWRFVEMGT
                     VNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
BASE COUNT          562 a          829 c          628 g          545 t
ORIGIN      
        1 acatcatcag cactgtaacc atattcacgc atcgaggcag ccgaacgggc aaacagggcc
       61 gcattatccg aaaatgcggt gcctgtccgc tggctgatat ccatcagcac tttctgtgat
      121 gacgcaaatt catcggatga ctgtgatgcc tgtttcagac gggcattcac ggagctccat
      181 tcatccgcca gcgaaatcag gtgtccggtg gcaaaggcac cggcaaacgc accggtcatt
      241 ccgacagccg aagcgcggat ttccgtcaac tggctgtgca gctcagccag agcccggcgc
      301 tgctccctgg ctgccgcagc agcctgacgt ccgccattct gcagggtccg gtaatattca
      361 ctgcccatac gggacgcccg ctggatctcc gactggaatg actgtgaatt tgccgaaatt
      421 ttgataatca gttcacgtaa cgtcgccatt cacctttctc cgggcgtaaa aaaaccgcct
      481 cagcggttct catcattcat gactgtgctg caaggctcag cgcgtcttcc agcgccgcaa
      541 acggatccac ctccggctta tcctcatcct cgccccagca gagcatggcg tccttcagtg
      601 caacattcat cccctgtgcc ccgaaaaccg ctttcacgat ctgtgcatta cggatatccc
      661 cgcgctcatc acccagcggg gatatcctgt cgaactccat ccacatcatc gcctcgctcg
      721 cactcaggct gtggcgcagt tcggataagg tgcgccccag acggagcgca agtcgcatca
      781 gaaagcgaat ttccgggcgg gctacttttt tctggccgac tctgcatcag cgatcagttc
      841 cagtgcctga cgcagcaacc gggcatgtac cggaccatag acggccagca cctgctcacg
      901 gtcgtccgga gtgaacaccc gctgcaggtc cgtatcacac aggacatcgc agaacagcgt
      961 cacatccgct tccaggttac ggcgggtttt cgccaccacc gacagggtat cgtcatcctc
     1021 tccatcacca ttgagcactt cctgccacag ataccaggcc tctgccgaag gctcccgcag
     1081 caccacgctg acattacccc attccggcac cttcaccgtt ttatgacgaa accctgacag
     1141 tctggccagc gccagcgttt tcagatcctt tttcatgatg acccatcccc ttatccggcg
     1201 gctgcgctca ctgtcacggt gcattcaaca gacgtcacac tctgtgcttt ctctgccgaa
     1261 tcggtcacca cacaggtata tttccccgca tcagcggact gcgcacctgg cttactgaag
     1321 gtgtctgtcg tctgcccgtc aaccggctga ccatccttct tccaggcgta tttatacggc
     1381 ggcgttcccc cgttggcact gactgacatt gtcagcagcg caccggtatt cacggtaagt
     1441 gtcttatcca gatttttcac aaacgccagc ggtaccacaa aggacaccgg tttgcctttc
     1501 agacgcagtg agaacgttgc agccaccacg ccgttggtac cggatgacca ggtgtgctgg
     1561 cgcacttccg ccaggaattt aaagccctta ccggacggaa acagcacctt aaacgcatac
     1621 aacgcgtcat tgtcataggc atcacgcagg gcgttctggg cctgattcag ataaaaattg
     1681 cccgacatgg aaatctcaga cgacgccccc agaccattga tgttctcctg ctctgtggag
     1741 cagagcgtgg tcacatcaat atcctgtttc tgcccggcgg tgaactgcac ttccttgatg
     1801 gtgcagtcca ggcgcagata ttccgcctta tccatagttt cagcagtcgc cggggcagat
     1861 gaaatcatca cctgcgtcag ctgtgagcgt tcatacaaag cagacattct gcctcctgat
     1921 aataaaaaac ccgcacgcgg cggggtatgg gttttgtaga aaaaagaaaa agtcacaccg
     1981 tgacctgaaa ctccagggtt gcacggtaac agcggttttc cggaatatag tcctgcattt
     2041 cactgacgga tcccggggcc agcagcatta tggcttcacg ggcgtcctga cgtatctgac
     2101 gcgcctgcgt cacagtcccg gcataaacgt ctatctgcac cgacactgag gactccgcct
     2161 gcccgcccat cacgtccgct gacaccgatg aaatcaggct gaaaaccacc cacggaagcg
     2221 ccaccgacgg cctgccatcc agcaggggga ccacatacgg gtacacctgc ccgccggcaa
     2281 gatgcgccag atgaggatac aaatccgcct ccgtcatcgt ctcagtacct catcaatggc
     2341 ccggttcatc cgcgcaatcg ccacctgtgc cgcctgttca ctgcgcacat caaatgccgg
     2401 gcgcacaaac gggtgcggtg gcatattcac ggtccccatt tccacaaacc gccagtagaa
     2461 agcgttgcgc gggttatccg ccttcatggt gttatcgctg ttaccggtgt ccggattaac
     2521 accccggata tgcacaccgg attccatccc gccatcgcgg gagc
//
LOCUS       DAZFBR010000259         2562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFBR010000259 DAZFBR010000000
VERSION     DAZFBR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.262"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            597..788
                     /locus_tag="ACHJZ2_005042"
     CDS             597..788
                     /locus_tag="ACHJZ2_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4570961.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(841..1074)
                     /locus_tag="ACHJZ2_005043"
     CDS             complement(841..1074)
                     /locus_tag="ACHJZ2_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570962.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(1170..1793)
                     /locus_tag="ACHJZ2_005044"
     CDS             complement(1170..1793)
                     /locus_tag="ACHJZ2_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4570963.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFRFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1882..2391)
                     /locus_tag="ACHJZ2_005045"
     CDS             complement(1882..2391)
                     /locus_tag="ACHJZ2_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570964.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          748 a          656 c          540 g          618 t
ORIGIN      
        1 tgagttcaca gataaaacac tctccaggaa aaccggggcg gttcaccagc ctcctgacga
       61 tacctcccac catgttttgc gatggcacgc taccgccctt ccggtattta cccgcatcaa
      121 ccggtaccag aatgctgagt aacaaaccag ctagaattga cttaattttg atcattatag
      181 aataatgatc tgaactacag caacatgaca gatttatcga tcgatataac acaaatgaac
      241 agttctttat tctgacaaat atcgcaaaaa cagcacatta caaaatttat aaccataatt
      301 actggaaaag tgcagaaaat tattccactg aatgctttta atacagttgg ttagcttaaa
      361 aaatagcaat ttttgatttc agtcattgaa agttgcaatg tttgacgatt caacagcatt
      421 taagtgagtt tcaggatatg ggaaggacat gaaaggagga gcgcaataaa atacacagga
      481 agaaggggaa aatgtttcgt aaaaacagaa aattaacagc gcacatcagg aaacgcagtc
      541 tcctgattca gtgctaccac atgaaaattt agttaactgt taccgtacta aaatttatgt
      601 cagaagatgt tccattaccg aaagtcaatc agcgatacaa agacgatcat ggtgcgcttg
      661 tcaccctgac cagtgttgag gaaacccggg tggtgtttat gcaggacggc tatccgcatc
      721 cctgcatgcg accgatgtac aactttctgg gaaaattcaa acccgaaccc cgggaagaaa
      781 cagaataaaa cgaaatccct gccggcaatc acactacctg atagcagcat aatccactta
      841 ttaattctca tccccggaga caagcccaga aaaccactcc gggtgttcac tgtatgccgt
      901 gttcagcgca tccctggaaa cgtttatata aaagctgtcc tgcaatttgc gggtaacaac
      961 ttctgccagg cgattccggt tatccaccag tccggcataa cctctctctg ccagccactc
     1021 attcagttca tgttccagag acagattgat aatatcataa tcggaccttg ccattcccgt
     1081 ttccttagca catgttttca ggcacgggga taaagtataa agaatgtcat actgctgctg
     1141 ccagagcaat attccccctg atggttgtat cagagatggt atgccggtac tttgcgggtt
     1201 gttcccgtca ggttatcgtg cacctccacg gtcgtggtca ccaccggcat tccggccttt
     1261 ctcagcctca gaacatcagc cgcaatacgc tgactgccaa acctgaacag gccgtccagt
     1321 gcagtcacca gtaaacccgc ctccagtgca tacttcagcc gttcacgggg cgctttcact
     1381 tcccgggcaa cctgctgata tggcaatgat gtgttttcat tcccaaccac gcgacgaata
     1441 cggtgagaca gatgataccg gtatgtatcc ggcacaccag aaaggctggc cttcaggctg
     1501 tacacgcagc caaaccgttt atcgttaaac accacatttt tctggctgat gccccattct
     1561 tcacgcagcg cggcaatcaa ttgcggtgta cgggtaagca gcaagcgaaa aggcagttca
     1621 aaactggtga cgtaatccac attcagcagt gcaatacgca accgttcttc tggcccggcc
     1681 tctgtctgcc ggcactcctc cagaacatcc tgccactgcc ggcgaagacg gggggattcg
     1741 ttccgttctg taaaacagta tttatccgcc agatagtcaa ttcgtgtacg catactgaaa
     1801 ggaattccgt ataaagattc agcagacaaa actttatcag ccagtaaaaa ttaacggaag
     1861 cattgatatt cctcccgaca atcaccggat gattgttgca ataacctgcg acataaagga
     1921 ctgaacagcc gtttttaacg ccacgtattg ccctgatgaa ccaggccggg ccacccgaaa
     1981 acatctttcc acccggcatt gtccgccagc acttatcacc gaccaggtct gttgcagtaa
     2041 atccaccagt tgcgcacatt cttcatcagc catgacgccg actcgcggat ggcaaatact
     2101 cgtctggctg agcagcaaca gacatcgtat ctgtgctcct tcatgttgtt cccgcaacca
     2161 gcgtaatacc accggcgctg atgggctgac cgtgtgatta tgctccgtaa tacgttcgtg
     2221 tacccgtcgg tgaaaggaac tacaaaatgt ctgaatctgt tgaccataga ttccttctgc
     2281 cgaatagagc gtgaagtgaa atgccaacag atttggccag tggtccaccg cctgctgaac
     2341 aaaacgccgg atttcccccg gctctgaaat taaggcttcg gttatttgca cgaaatgttc
     2401 cttttcaacc cctgttgaat tttgtcaagc cggtgcagac acagtaaagc gcgcatcaac
     2461 acctgaaagg tacgatacaa acacaagtta caaacaataa gaacggaagg acaattaatc
     2521 agtgaggaat aataactaaa ttaaccaaaa gaatgttact ga
//
LOCUS       DAZFBR010000260         2543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFBR010000260 DAZFBR010000000
VERSION     DAZFBR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.263"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..881
                     /locus_tag="ACHJZ2_005046"
     CDS             <1..881
                     /locus_tag="ACHJZ2_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4570965.1"
                     /translation="DGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESE
                     LDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQT
                     AQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRK
                     FVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLK
                     EVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFES
                     GLRQSTEEEKSDSRAA"
     gene            862..2181
                     /locus_tag="ACHJZ2_005047"
     CDS             862..2181
                     /locus_tag="ACHJZ2_005047"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4570966.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            2191..2523
                     /locus_tag="ACHJZ2_005048"
     CDS             2191..2523
                     /locus_tag="ACHJZ2_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4570967.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
BASE COUNT          550 a          708 c          811 g          474 t
ORIGIN      
        1 aggacgggca gacccgcggt gcaaatgtgt tttacagcgt gatggagcag atgaagatgc
       61 tcgacacgct gcagaacacg cagctgcaga gcgccattgt gaaggcgatg tatgccgcca
      121 ccattgagag tgagctggat acgcagtcag cgatggattt tattctgggc gcgaacagtc
      181 aggagcagcg ggaaaggctg accggctgga ttggtgaaat tgccgcgtat tacgccgcag
      241 caccggtccg tctgggaggc gcaaaagtgc cgcacctgat gccgggggac tcactgaacc
      301 tgcagacggc tcaggacacg gataacggct actccgtgtt tgagcagtca ctgctgcggt
      361 atatcgctgc cgggctgggt gtctcgtatg agcagctttc ccggaattac gcccagatga
      421 gctactccac ggcacgggcc agtgcgaacg agtcgtgggc gtactttatg gggcggcgaa
      481 aattcgtcgc atcccgtcag gcgagccaga tgtttctgtg ctggctggaa gaggccatcg
      541 ttcgccgcgt ggtgacgtta ccttcaaaag cgcgcttcag ctttcaggaa gcccgcagtg
      601 cctgggggaa ctgcgactgg ataggctccg gtcgtatggc catcgatggt ctgaaagaag
      661 ttcaggaagc ggtgatgctg atagaagccg gactgagcac ctacgagaaa gagtgcgcga
      721 aacgcggtga cgactatcag gaaatttttg cccagcaggt ccgtgaaacg atggagcgcc
      781 gcgcagctgg tcttaaaccg cccgcctggg cggctgcggc atttgaatcc gggctgcgac
      841 aatcaacaga ggaggagaag agtgacagca gagctgcgta atctcccgca tattgccagc
      901 atggctttta atgagccgct gatgcttgaa cccgcctatg cgcgggtttt cttttgtgcg
      961 cttgcaggcc agcttgggat cagccgcctg acggatgcag tatccggcga cagcctgact
     1021 gccggagagg cacccgcggc gctggcgtta tccggtgatg atgacggacc acgacaggcc
     1081 cggagttatc aggtcatgaa cggcatcgcc gtgctgccgg tgtccggtac gctggtcagc
     1141 cggacgcggg cgctgcagcc gtattcggga atgaccggtt acaacggcat tatcgcccgt
     1201 ctgcaacagg ctgccagcga tccgatggtg gacggcattc tgctcgatat ggacacaccg
     1261 ggcgggatgg tggcgggagc atttgactgt gctgacatca tcgcccgtgt gcgagacata
     1321 aaaccggtat gggcgctggc caacgacatg aactgcagtg caggtcagct gcttgccagc
     1381 gccgcctccc ggcgtctggt cacgcagacc gcccggacag gctccatcgg cgtcatgatg
     1441 gctcacagta attacggtgc tgccctggag aaacagggcg tggaaatcac gctgatttac
     1501 agcggcagcc ataaggtgga tggcaacccc tacagccatc taccgggtga tgtccgggaa
     1561 acactgcagt cccggatgga tgcaacccgc cggatgtttg cgcagaaggt gtcggcatat
     1621 accggcctgt ccgtgcaggc tgtgctggat accgaggctg cagtgtacag cggtcaggag
     1681 gccattgatg ccggactggc tgatgaactt gtcaacagca ccgatgcgat caccgttatg
     1741 cgtgatgcac tggatgcacg taaatcccgt ctctcaggag ggcgaatgac caaagagact
     1801 caatcaacaa ctgtttcagc cactgcttcg caggctgacg ttactggcgt ggtgccagcg
     1861 acggagggcg aaaacgccag cgcggcgcag ccggacgtga acgcgcagat caccgctgcg
     1921 gttgcggcag aaaacagccg cattatgggg atcctcaact gtgaggaggc tcacggacgc
     1981 gaagaacagg cccgcgtgct ggcagaaacc cccggtatga ccgtggaaac ggcccgccgc
     2041 attctggccg cagcaccaca gagtgcacag gcgcgcagtg atactgcgct ggatcgtctg
     2101 atgcaggggg caccggcacc gctggctgca ggtaacccgg catctgatgc cgttaacgat
     2161 ttgctgaaca caccagtgta agggatgttt atgacgagca aagaaacctt tacccattac
     2221 cagccgctgg gcaacagtga cccggctcat accgcaaccg cgcccggcgg attgagtgcg
     2281 aaagcgcctg caatgacccc gctgatgctg gacacctcca gccgtaagct ggttgcgtgg
     2341 gatggcacca ccgacggtgc tgccgttggc attcttgcgg ttgctgctga ccagaccagc
     2401 accacgctga cgttctacaa gtccggcacg ttccgttatg aggatgtgct ctggccggag
     2461 gctgccagcg acgagacgaa aaaacggacc gcgtttgccg gaacggcaat cagcatcgtt
     2521 taacctgacc cttcatcact aaa
//
LOCUS       DAZFBR010000261         2508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFBR010000261 DAZFBR010000000
VERSION     DAZFBR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.264"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(112..801)
                     /locus_tag="ACHJZ2_005049"
     CDS             complement(112..801)
                     /locus_tag="ACHJZ2_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4570968.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(816..938)
                     /locus_tag="ACHJZ2_005050"
     CDS             complement(816..938)
                     /locus_tag="ACHJZ2_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4570969.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            1276..2235
                     /locus_tag="ACHJZ2_005051"
     CDS             1276..2235
                     /locus_tag="ACHJZ2_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO4570970.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
     gene            complement(2379..2450)
                     /locus_tag="ACHJZ2_005052"
                     /pseudo
     CDS             complement(2379..2450)
                     /locus_tag="ACHJZ2_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
BASE COUNT          802 a          546 c          458 g          702 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaccc actcggcagc
       61 gggtttatac attttttaca acataccaaa tttgcatgaa gtatatggct tttaatccag
      121 ttttgcaata ttttgctgta aaaatgctgc cttttgtttt gaacgtgttc tcgtcacaag
      181 caataaagca tcactatcaa gctgtagaaa aatgtgcttc attgcaaccc agcgttcagt
      241 aaatgtctcg gaccagtttt ttgttgtcac tcccaccaat gatgccagtg tctggtattc
      301 ataggcctca cgccctgcaa gttcgctctt cacatcctgt gcagccagcc agattaacgt
      361 cttcaggcga tccagtgtct tacctgcaat ttttctgtta cttaacaaat ctttaaactc
      421 gctccatgcc cattgcgtta tggtgacctg atgcccccat cgaacgcttt cgctgtaaca
      481 ccaaagcaac catgctttct gatgttcatc gagagacaaa accgcgcggc gccatgaaga
      541 ggttgagaat tcaaccgggc tgaccaaagc aatggatgaa ccttttgcgt acgactgctt
      601 accggaagtc ggcgtattat ccagcgtaat catcttgcca gttaccacat ccagaatgcg
      661 cggcttcttt cgtttgtatg taccagtatc aaattgtgca tgctcctgcc aggcttcgag
      721 ctggcctttc gttgctccgt tcaagtcagc agtagctgcc ataagttgct cacgaacata
      781 ctgtaaatat tgggtattca tgcagtaaat cctttctata ttttggcata attcttcaac
      841 attcggtaat cgttcaaaac cgaatcgggg aaacgacata agcacaggag cccccagcga
      901 cagcgaagga gttctgatat ataagactca gacatcattc attccccggt tctccaatat
      961 ctgtttcact catcatccat aactacctgt aattgccccc cctttttttg taacagttct
     1021 tatattgcta tatagaatag ccattactaa tgcatttaaa tttaataaaa taaaattata
     1081 aaaaacataa aacaccacgc aaacacactt aataaaaaca ccgttacatt aaaagataat
     1141 aaaaaccgca ataaaaaacg aataaatcaa ttgtctcacg caattataaa acatcatatt
     1201 gattacgcac cttgcattac aaactcatgt atgtaaaata cgcgcaccat tcaaaaaaaa
     1261 ggaagacaat aacatatgaa aaaaagtgtc atcgctggcg tctttattgc tctgtcattt
     1321 accacgtgtt cagctatcgc gaacagcctt gcattatcat tagcaaatga tgatgcaggg
     1381 aagtttcaac caatacttaa tgatatttat ggcaataaac atgaaaacag agatgattac
     1441 tcacaaggct tatttctggg atatagccac gatatctcag actcgagcca attatctctc
     1501 catattgcgc aagatattta ctctccatca ggcagtaata aaagacacaa cacagctgta
     1561 actggagaca gagcttttag tgcatacact cacactggta ttgaatggaa ctcccttgcg
     1621 aatgactgga ttcgctatcg attaggtact gacataggtg ttgttggccc cgacgcaggc
     1681 ggtcagaaag tacaaaataa agctcatgag attattgggg cagaaaaata tcatgcatgg
     1741 gatgatcaaa tagagaatcg ctacggttat actgtaaaag ggatgctatc catgacacca
     1801 agtatggata ttttaggtgc taatgttgga ttataccctg aagtttctgc tgttactgga
     1861 aacttatttc aatatgtagc atatggcgca accattgcca ttggtaatga taaaaccttc
     1921 aattcggata atggctttgg tctgctggct ccccgtggtt taatgcatat gtccgataca
     1981 agcggattca aatacaagat ttttgcaggt atggaaagac gagatgtcaa tcgcaactat
     2041 actctcgaag gaaaaacaat acagacgaaa caaacaacag tatcgctaaa caaaactgtt
     2101 gatgaatatc aagttggcgc aacaattggg tatgcacctg tagccttcac actagcattt
     2161 aataaagtaa catcagaatt caagacaggg gatgactatt catttataaa tggagcaatc
     2221 accttctttt tttaactgaa ttgaattcaa tcaaaataac ataagtccaa caaaaacata
     2281 aagtgcgaaa tgaatgccag ctccatttat ttcgcactat aaaagattaa aagttgcaat
     2341 aaaataataa aatgactcag ttacgaaaac caataaactg tggccagtag tgagtcgctc
     2401 atcatcgggc tttttggcga atgaaattta gctacgcttt cgagtctcat gcgccttctc
     2461 cctgtacctg aatcaatgtg aggtttccgc agaacactgc gccagtat
//
LOCUS       DAZFBR010000262         2502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFBR010000262 DAZFBR010000000
VERSION     DAZFBR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.265"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..569
                     /locus_tag="ACHJZ2_005053"
     CDS             <1..569
                     /locus_tag="ACHJZ2_005053"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4570971.1"
                     /translation="YFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYL
                     KLQSLFARVAEEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVP
                     YTDVWTHKPVQYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALG
                     RRATGVELETERFEQTVREVQDLASQNG"
     gene            610..685
                     /locus_tag="ACHJZ2_005054"
     tRNA            610..685
                     /locus_tag="ACHJZ2_005054"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:643..645,aa:Ile,seq:cat)
     gene            695..771
                     /locus_tag="ACHJZ2_005055"
     tRNA            695..771
                     /locus_tag="ACHJZ2_005055"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:729..731,aa:Arg,seq:tcg)
     gene            785..861
                     /locus_tag="ACHJZ2_005056"
     tRNA            785..861
                     /locus_tag="ACHJZ2_005056"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:819..821,aa:Arg,seq:tct)
     gene            952..1911
                     /locus_tag="ACHJZ2_005057"
     CDS             952..1911
                     /locus_tag="ACHJZ2_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO4570972.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1923..2192
                     /locus_tag="ACHJZ2_005058"
     CDS             1923..2192
                     /locus_tag="ACHJZ2_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO4570973.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          641 a          546 c          667 g          648 t
ORIGIN      
        1 cttactttcg tgatgcgcgt gctgccctgg ggataacggc aaaacagata gtggatgcca
       61 caggaaagaa aaacatggtg tcgcactggt tcagtgccag tcagtggcag ctaccgaacg
      121 aaagcgatta tctgaaatta cagtcgctgt ttgcccgggt ggcagaagag aagcatcagc
      181 gcggtgaact ggaaaagccc caccaccagc tgctggagac gtatacttca ctgaaccggc
      241 agtatgcgga actgcagagt gaatataagc atctgcggcg gtattttggc gtgacggcgc
      301 aggtgccgta cacggatgtg tggacgcata aaccggtgca gtactatccc gggaaacatc
      361 cgtgcgaaaa accggcagaa atgctgcagc agataatcag cgcgagcagt cgtccgggtg
      421 acctggttgc agattttttc atggggtcgg gttcgacagt caaagctgca ctggcgctgg
      481 ggcgtcgtgc aactggcgtt gagctggaga ctgaacgttt tgagcagacg gtcagggaag
      541 ttcaggattt agccagtcag aacggatgat attgcaggat tagttacgta ccgttattat
      601 cctgcgcccg gccctttagc tcagtggtga gagcgagcga ctcataatcg ccaggtcgct
      661 ggttcaaatc cagcaagggc caccatatca cataccgcca ttagctcatc gggacagagc
      721 gccagccttc gaagctggct gcgcggggtt cgagtcctcg atggcggtcc attatcggta
      781 ttcagcgttg ttagctcagc cggacagagc aattgccttc taagcaatcg gtcactggtt
      841 cgaatccagt acaacgcgcc atatttattt accaggctcg cttttgcggg ccttttttat
      901 atctgcgccg ggtctggtgc tgattacttc agccaaaagg aacacctgta tatgaagtgt
      961 atattattta aatgggtact gtgcctgtta ctgggttttt cttcggtatc ctattcccgg
     1021 gaatttacga tagacttttc gactcaacaa agttatgtct cttcgttaaa tagtatacgg
     1081 acagagatat cgacccctct tgaacatata tctcagggga ccacatcggt gtctgttatt
     1141 aaccacaccc cgccgggcag ttattttgct gtggatatac gagggcttga tgtctatcag
     1201 gcgcgttttg accatcttcg tctgattatt gagcaaaata atttatatgt ggccgggttc
     1261 gttaatacgg caacaaatac tttctaccgt ttttcagatt ttacacatat atcagtgccc
     1321 gatgtgacaa cggtttccat gacaacggac agcagttata ccactctgca acgtgtcgca
     1381 gcgctggaac gttccggaat gcaaatcagt cgtcactcac tggtttcatc atatctggcg
     1441 ttaatggagt tcagtggtaa tacaatgacc agagatgcat ccagagcagt tctgcgtttt
     1501 gtcactgtca cggcagaagc cttacgcttc aggcagatac agagagaatt tcgtcaggca
     1561 ctgtctgaaa ctgctcctgt gtatacgatg acgccgggag acgtggacct cactctgaac
     1621 tgggggcgaa tcagcaatgt gcttccggag tatcggggag aggatggtgt cagagtgggg
     1681 agaatatcct ttaataatat atcagcgata ctgggtactg tggccgttat actgaattgc
     1741 catcatcagg gggcgcgttc tgttcgcgcc gtgaatgaag agagtcaacc agaatgtcag
     1801 ataactggcg acaggcccgt tataaaaata aacaatacat tatgggaaag taatacagct
     1861 gcagcgtttc tgaacagaaa gtcacagttt ttatatacaa cgggtaaata aaggagttaa
     1921 gtatgaagaa gatgtttatg gcggttttat ttgcattagt ttctgttaat gcaatggcgg
     1981 cggattgtgc taaaggtaaa attgagtttt ccaagtataa tgaggatgac acatttacag
     2041 tgaaggttga cgggaaagaa tactggacca gtcgctggaa tctgcaaccg ttactgcaaa
     2101 gtgctcagtt gacaggaatg actgtcacaa tcaaatccag tacctgtgaa tcaggctccg
     2161 gatttgctga agtgcagttt aataatgact gaggcataac ctgattcgtg gtatgtgggt
     2221 aacaagtgta atctgtgtca caattcagtc agttgacagt tgcctgtcag actgagcatt
     2281 tgttaaaaaa atttcgcatg gtgaatcccc ctgtgcggag gggcgactgg tgtataaggt
     2341 gtcatctctg acgacaacct ggatcgagat acgcgggttc agggacaccg gactgaactc
     2401 accgggaggc acccggcacc atgttcatgg tgatacagaa atgcggcttc agcccctctc
     2461 cggaggggct ttttatctga atgattctgt tattttcgcc cg
//
LOCUS       DAZFBR010000263         2455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFBR010000263 DAZFBR010000000
VERSION     DAZFBR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.266"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            310..516
                     /locus_tag="ACHJZ2_005059"
     CDS             310..516
                     /locus_tag="ACHJZ2_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4570974.1"
                     /translation="MEKITTGVSYTTSAVGTEYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(772..1044)
                     /locus_tag="ACHJZ2_005060"
     CDS             complement(772..1044)
                     /locus_tag="ACHJZ2_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570975.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1204..1737
                     /locus_tag="ACHJZ2_005061"
     CDS             1204..1737
                     /locus_tag="ACHJZ2_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4570976.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1958..2071
                     /locus_tag="ACHJZ2_005062"
     CDS             1958..2071
                     /locus_tag="ACHJZ2_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570977.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            2073..2393
                     /locus_tag="ACHJZ2_005063"
                     /pseudo
     CDS             2073..2393
                     /locus_tag="ACHJZ2_005063"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2335..>2455
                     /locus_tag="ACHJZ2_005064"
     CDS             2335..>2455
                     /locus_tag="ACHJZ2_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4570978.1"
                     /translation="MVVSACGSTPPAPVPCVKPPAPPAWIMQPAPDWQTPLNGI"
BASE COUNT          630 a          498 c          675 g          652 t
ORIGIN      
        1 ctttattaag gaaaaaaaca gccagcactg actttcggtg gagaggtgct ggctcagaag
       61 gatagttgga tttcacatga tacttatgcc tggcggtata ttttctgaca gacagtgacg
      121 ggtgttgtca agatattgtg tcatttataa cctgaatcag gggaggccgg aatgttatct
      181 ggcattttta gcagagcctg aatgccataa tcacggctcc cggagttggc cgtcagtggg
      241 tgacactggc ggcttttttg tttttcttta ctttcatttt ctgtcggcgg tgacggagac
      301 atacatcaga tggaaaaaat cacaacaggt gtgtcataca ccacgtcagc ggtggggacg
      361 gaatactggt tactgcagct gctggacaaa gtctctccgt cccagtgggt ggcaataggt
      421 gtgctgggaa gtctgctgtt tggcctgctg acgtatctga ctaacctgta tttcaaaatc
      481 agagaggacc gtcgtaaggt ggcgcgggga gagtagtcga tgaataaaca atacgaactg
      541 gttgtaaaat gaatatttct aactgaaaaa acgttccatg aggtaagaaa aggtcacagg
      601 caatcaataa caggacgtga tgaaagaccc ttgcatttgt gcgctttctc tttagatagc
      661 agcagatact gaaaatctga gttgtcgggg agtcagggat acagctgtgc aagagttggt
      721 cattgtgatt ccattgaaat cctgtatgcc atgaagggca ggattttatg gctacctgag
      781 ctttggtgat agtaagttga aaattcgcat tttttgctga catgcgtaac gagaatccca
      841 taagcaggga ggacttaatt cttcattaac ccatgcgttg atattatgtt tcagccgttg
      901 aagcatcagc ggtgttaatg ttgtggtaat aatatccagc gttttatgtg agatcttacc
      961 gtaagggtct gcaagaatgc tgcttgttgc ttcgttatta tctgccatca gaagaagtaa
     1021 ctctgattta acgttttctg tcattagttg taaaaatctt ctgcgcaaac tttctttact
     1081 gttcatttat atggcttcat ttgttgtaat ctgctgcgtc tcaagggata tgtttatgag
     1141 agcgaccatg agtgttggat tatataccta acatatcaag ggattagaaa tcgataaatc
     1201 cccatgaacg aaaaaataaa atacggcctg tcggctgccg ttctggcgct gattggtgca
     1261 ggtgcttctg cgcctgaaat cctcgaccag tttctggatg aaaaggaagg taaccacacc
     1321 acggcatacc gtgatggtgc ggggatctgg accatctgcc gtggtgccat tctggtggat
     1381 ggtaagcctg ttattcctgg catgaagctg tcaaaggaaa aatgcgaccg ggttaatgcc
     1441 atcgaacgtg acaaggcgct ggcatgggtg gagaaaaaca tccgggtgcc gctgaccgaa
     1501 ccccagaaag cggggatcgc gtcattctgt ccgtacaaca ttggccccgg taagtgcttc
     1561 ccgtcgacgt tttataaacg aattaatgca ggcgatcgaa aaggtgcctg tgaggcgatt
     1621 cgctggtgga ttaaggacgg tggcagagac tgccgtatcc gttcaaataa ttgctacggt
     1681 caggtctcac ggcgtgacca ggagagcgcg ctggcgtgct gggacatcga cagatagcag
     1741 aatattttcc tgaaaaatga cgttggccaa cgcgggtgga taacacgaaa tcctgaaaac
     1801 tggtaaaacc taagtgaata aaagtaaaaa ccccgtttgt tggcagcaag cggggttttg
     1861 tgttttctga ccttgagtaa ggcaagggag aaattatggg tagggaggta ctttccctgt
     1921 gaggaagtat aaaagattct ttctgaggtt gtccattatg aaaggcattg aagtggagac
     1981 gccagccagt ctggatttaa caagagcggc agcttttgcc attcgtattg tggccattgc
     2041 tgttctggtc tgggcaatcc gttggtggtg atatgaaccg tgttctgtgt gtggttatca
     2101 ttgtcctgct ggtggcctgt ggtgcgctta gtctggggct gaatcattac cgtgatcacg
     2161 ccatcatcta caaagagcag cgcgataaaa aagccagtga gctggagctg gcgaacgcga
     2221 caattactga tatgcagata cgccagcgtg atgtcgctgc acttgatgcc agatactcga
     2281 gggaattagc cgatgcgaga gctgaaaatg aaactctgcg tgctgatgtt gccgctggtc
     2341 gtaagcgcct gcggatcaac gccacctgct ccggtaccgt gcgtgaagcc accggcacct
     2401 ccggcgtgga taatgcaacc ggcccccgac tggcagacac cgctgaacgg gatta
//
LOCUS       DAZFBR010000264         2430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFBR010000264 DAZFBR010000000
VERSION     DAZFBR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.267"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1199
                     /gene="escC"
                     /locus_tag="ACHJZ2_005065"
     CDS             <1..1199
                     /gene="escC"
                     /locus_tag="ACHJZ2_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO4570979.1"
                     /translation="YLDIDSLLKYLSDTISVNKNSCNVRKITTFNSIEVRGVPECIKY
                     ITSLSESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILKTMAS
                     NGSLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELDIEQR
                     QIEISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNASNFMI
                     RVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRV
                     TPRILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEAT
                     LSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPI
                     KSASSE"
     gene            1213..1668
                     /gene="cesD"
                     /locus_tag="ACHJZ2_005066"
     CDS             1213..1668
                     /gene="cesD"
                     /locus_tag="ACHJZ2_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO4570980.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
     gene            complement(2052..>2430)
                     /gene="grlA"
                     /locus_tag="ACHJZ2_005067"
     CDS             complement(2052..>2430)
                     /gene="grlA"
                     /locus_tag="ACHJZ2_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFO4570981.1"
                     /translation="PDSVKNYDGEPLYILVSLWCKLQEKWISRNDIAEAFGINLRRAS
                     FIITYISRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESPGTTGPKRKTYR
                     VGNGIVGQSNIWNEMIMRRKKES"
BASE COUNT          801 a          471 c          439 g          719 t
ORIGIN      
        1 catatctgga tattgactca ctgctaaaat atctgagtga tacaatctca gtaaacaaaa
       61 actcttgcaa tgtgagaaaa attacgacat ttaactcgat tgaagttcgg ggtgttcctg
      121 aatgtataaa atatattaca tctttatcag aaagtcttga taaagaggca cagtctaaag
      181 ccaaaaataa agatgttgtt aaggtcttta aactgaacta tgcgtcagcg acagatataa
      241 catacaaata ccgtgaccaa aatgttgtcg tcccaggtgt tgtctccatt ctgaagacaa
      301 tggcaagtaa tggttcactt cccagtaccg gtaaaggtgc cgtcgagcga agcggtaacc
      361 tgtttgataa cagtgtaacg atttccgctg atccacgtct taatgcagtt gttgtaaaag
      421 accgcgaaat aacgatggat atctatcagc aattaatttc tgagcttgat attgaacaaa
      481 ggcaaattga aatttctgtc tcaattattg atgttgatgc aaacgatctt cagcagctcg
      541 gcgtaaactg gtccggtacg ttaaatgcag gacaaggtac gattgccttt aatagctcta
      601 ccgcacaggc taatatcagc tcttctgtga taagtaatgc cagtaatttt atgattaggg
      661 tgaatgcgtt acaacagaac tcgaaagcta aaatactctc tcagccttca attataacct
      721 taaataatat gcaggcgatt cttgataaaa acgtaacgtt ttatacaaaa gtctccggag
      781 aaaaggtggc gtctctggaa agtattacaa gcggaacatt gctgagagtg acaccacgta
      841 tacttgatga cagctcgaat agccttaccg gaaaacgtcg ggaacgagtt cgtttgttac
      901 ttgatattca ggatggcaat caatcgacta accaaagcaa tgctcaggat gccagcagca
      961 ccttacctga agtacaaaat tcagaaatga caacagaagc gacattaagt gcaggagaaa
     1021 gtttattact tggcggattt atccaagata aagaaagttc aagtaaagat ggtataccgc
     1081 tgttaagcga cattcctgtt attggtagtt tattctccag tacggtaaaa caaaaacata
     1141 gtgttgttcg actcttttta attaaagcaa ccccgataaa atctgcatct agcgaataat
     1201 aaatgaggct ttatgagcag gaaatttagc tctctagagg atatttatga tttctaccag
     1261 gatggtggca cattagcgtc attaacaaat ctgacacaac aagatctcaa tgaccttcat
     1321 tcttatgcct atacagcata tcaatctggt gatgttataa ccgcaagaaa tctattccat
     1381 ttgctcacat atctggaaca ctggaattat gactacacct tatctctggg cttatgtcat
     1441 cagcgtttat caaatcatga agatgcacaa ctgtgtttcg cacgctgtgc aactttagtt
     1501 atgcaagatc ccagggcatc ttattattct ggaattagct acttactcgt cggaaataag
     1561 aaaatggcca agaaagcctt taaggcttgt ttaatgtggt gtaatgaaaa agaaaaatac
     1621 actacatata aagaaaatat taaaaaattg ttaggtaata cagagtaaaa actaccatca
     1681 gctataccaa tccccaggct gactggggat tttctatcgg aaataataaa gctgctatag
     1741 tgaagtgctc ccaaagttga gcaccgacca ctggtagatt tatctacagg tgtggggtta
     1801 ttccattaac caggagttta tgccctgaac tatcttctgc tgtatttaat atcatttccg
     1861 tttatgaaac aatcagtata tcttaactta gcattagaaa tctgatcttt ccaatcatct
     1921 atagctcaga gtatatttgt caggaaatga ccttcaaact ttagccgaga aaaaggctta
     1981 ccctggaaaa caaaaccctt aaatatagct ttatttttat tcttctataa aatatactca
     2041 aaaaattacg tctaactctc ctttttccgc ctcatgatca tttcgttcca gatattagac
     2101 tgtcccacaa taccattacc aactcggtag gtttttcttt ttggtccggt tgttccagga
     2161 ctttctatcg gaaccgcctc aaggttaaca tcataaatga aaatctcaag gcgcttataa
     2221 tgcaaattac cataactaac atatctgaca cgaaatgaaa ttttttcttt tcttctcgat
     2281 atataagtta taataaatga tgctctcctc aggtttatac cgaatgcttc ggcaatatca
     2341 ttgcgagaaa tccatttctc ctgcaattta caccaaagag aaaccaagat atacagaggt
     2401 tcaccatcgt aattctttac tgagtcagga
//
LOCUS       DAZFBR010000265         2430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFBR010000265 DAZFBR010000000
VERSION     DAZFBR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.268"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(93..168)
                     /locus_tag="ACHJZ2_005068"
     tRNA            complement(93..168)
                     /locus_tag="ACHJZ2_005068"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Val,seq:tac)
     gene            427..1842
                     /gene="gltX"
                     /locus_tag="ACHJZ2_005069"
     CDS             427..1842
                     /gene="gltX"
                     /locus_tag="ACHJZ2_005069"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO4570982.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(1960..2385)
                     /gene="tnpA"
                     /locus_tag="ACHJZ2_005070"
                     /pseudo
     CDS             complement(1960..2385)
                     /gene="tnpA"
                     /locus_tag="ACHJZ2_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          620 a          598 c          628 g          584 t
ORIGIN      
        1 gaccccctcc ttgtaaggga ggtgctctcc cagctgagct aatcacccac ttctcaattt
       61 cttgctacac ggcggagact acataaagta gttggtgggt gatgacggga tcgaaccgcc
      121 gaccccctcc ttgtaaggga ggtgctctcc cagctgagct aatcaccccc gctgtgtgga
      181 gtcgcattat agggagagtt caaaatgagt caacgcattt tctaaagaaa ttgttcgttc
      241 gtcgtaaatt taagcaagat gatcgcaaaa cagaccgtgt tgcgcaattt gtcaacgaaa
      301 acaataatgc gtaaggttgc aacccgaact acattgagga atcaggcggg agtgatagaa
      361 tatcgcccac tcaatttttc caggatttgc cggttgtcgg catctttcta aacgtaaggc
      421 catttcatga aaatcaaaac tcgcttcgcg ccaagcccaa caggctatct gcacgttggc
      481 ggcgcgcgta ctgctcttta ctcctggctt tttgcacgta atcacggcgg tgagttcgtg
      541 ctgcgtattg aagacaccga tcttgagcgt tccacgccgg aagctatcga agccattatg
      601 gatggcatga actggctgag cctggagtgg gatgaaggtc cgtacttcca gaccaaacgt
      661 tttgatcgct acaacgcggt gatcgatcag atgctggaag agggcacagc ttataaatgc
      721 tattgctcta aagagcgcct ggaagcgctg cgcgaagagc aaatggcgaa aggtgagaag
      781 ccgcgttatg acggtcgctg ccgccacagt catgagcatc acgctgatga tgagccgtgt
      841 gtcgtacgtt ttgctaaccc gcaggaaggt tctgttgttt ttgacgatca gatccgtggt
      901 ccgatcgagt tcagcaacca ggaactggac gatcttatta tccgccgtac cgatggttcc
      961 ccaacctata acttctgtgt ggttgtcgat gactgggata tggaaatcac ccacgttatc
     1021 cgtggcgaag accatatcaa caacacgcca cgccagatca acattcttaa agccctgaaa
     1081 gcgccggtac cggtttacgc gcacgtttct atgatcaacg gcgatgacgg caaaaaactg
     1141 tccaaacgtc acggggcagt cagcgtaatg cagtatcgtg atgacggtta tttgccagaa
     1201 gcactgctga actatctggt gcgtctgggc tggtcccacg gcgatcagga aatcttcact
     1261 cgtgaagaga tgatcaaata cttcactttg aatgccgtca gcaaatctgc cagtgcgttc
     1321 aacaccgaca agctgctgtg gctgaaccat cactacatta acgcgctgcc gccggagtat
     1381 gttgctactc acttacagtg gcacattgag caggaaaata tcgatacccg taacggcccg
     1441 cagctggctg atctggtgaa actgctgggc gaacgctgca agacgctgaa agagatggca
     1501 cagagctgcc gttatttcta cgaagatttt gctgagttcg atgccgacgc cgcgaaaaaa
     1561 catctgcgtc cggtagcgcg tcagccgctg gaagtggttc gtgacaaact ggccgcgatt
     1621 actgactgga cagctgaaaa cgttcatcac gctattcagg cgacggcgga tgagctggaa
     1681 gtgggtatgg gtaaagttgg tatgccgctg cgtgtcgccg taaccggtgc ggggcagtct
     1741 ccggcgctgg acgttaccgt tcacgcgatc ggtaagaccc gcagtatcga gcgtatcaac
     1801 aaagcgctgg cttttattgc ggaacgcgaa aatcagcagt aattagcgca taaaagataa
     1861 acggcaggag agatacccct gccgtttttt atttatccgg tgcgccgtaa aaccccgtcc
     1921 ttcagggcgg ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat
     1981 gtattggcgg atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact
     2041 ccacaaaacg cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa
     2101 cctacccgat acgcctttga ggctgtttac cagactggat atcgccagtt tgggaggata
     2161 gttagttaac aaatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa
     2221 atcagcacat acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat agtcaaaaat
     2281 ctgacgtcgg tatctggtga caaagaacag atgaacatgc atcaggaaaa cacaatgcct
     2341 tccccgtcgg atatcggttt ctttattcat agaccaaagt atcatttggc ctgtgaaacg
     2401 actacaggca tttaaattcc agttaagacc
//
LOCUS       DAZFBR010000266         2427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFBR010000266 DAZFBR010000000
VERSION     DAZFBR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.269"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            50..2317
                     /gene="adiA"
                     /locus_tag="ACHJZ2_005071"
     CDS             50..2317
                     /gene="adiA"
                     /locus_tag="ACHJZ2_005071"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO4570983.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          611 a          661 c          604 g          551 t
ORIGIN      
        1 gaagatactt gcccgcaacg aagattcctt cataaccggg taagcaatga tgaaagtatt
       61 aattgttgaa agcgagtttc tccatcaaga cacctgggtc ggtaacgctg ttgagcgtct
      121 ggcagatgct ttaagccagc aaaatgttac cgtgattaaa tccacctcct ttgatgatgg
      181 ttttgccatt ctctcttcaa acgaagccat tgactgcctg atgttcagct atcaaatgga
      241 acatccggac gaacatcaaa acgtcagaca attgatcggt aagcttcatg agcgccaaca
      301 aaacgtgccg gtcttcctgt tgggcgatcg ggaaaaagcc ctcgccgcaa tggaccggga
      361 tctgctggag cttgtcgatg aattcgcctg gattctggaa gataccgccg actttatcgc
      421 cggacgcgcc gttgccgcga tgacccgcta ccgccagcag ctgttgccgc cactgttcag
      481 cgcgctgatg aaatatagcg acatccatga atattcctgg gcagcgccag gccaccaggg
      541 cggcgttggt tttaccaaaa cacccgccgg acgtttctac catgactact acggtgaaaa
      601 tctgttccgc accgacatgg gcatcgaacg aacttccctc ggttctttgc ttgaccatac
      661 tggcgcattt ggcgaaagcg aaaaatatgc cgcacgcgta tttggtgccg atcgctcctg
      721 gtcggtagtc gtcggtactt ccggctctaa ccgcaccatc atgcaggctt gcatgaccga
      781 taacgatgtc gtggtcgttg accgtaactg ccataaatcc atcgaacaag gtttgatgct
      841 gacaggcgcg aaaccggtct acatggtgcc aagccgcaac cgctacggca ttatcgggcc
      901 aatctatccg caggaaatgc aacctgaaac cttgcagaag aaaatcagtg aaagcccgct
      961 gactaaagac aaagccgggc aaaaaccgtc ttactgcgtg gtgaccaact gcacctatga
     1021 cggcgtgtgt tataacgcta aagaagcgca ggatctgctg gaaaaaacct ccgatcgtct
     1081 gcactttgac gaagcctggt acggctatgc acgtttcaac ccgatctatg ccgatcacta
     1141 tgccatgcgc ggcgaacctg gcgatcacaa cggtcctacc gttttcgcca cccactccac
     1201 ccacaaactg ctgaatgcgc tttcacaggc ctcttatatt catgtacgtg aaggtcgtgg
     1261 ggcgattaac ttctcccgct tcaaccaggc ctacatgatg catgccacca cctccccgct
     1321 gtatgccatc tgcgcatcca acgacgtggc ggtgtcgatg atggacggca acagcggcct
     1381 gtcactgacg caggaagtga tcgacgaagc ggttgatttc cgtcaggcga tggcgcggct
     1441 gtataaagag ttcgccgctg acggtagctg gttcttcaaa ccgtggaaca aagaagtcgt
     1501 caccgaccca caaaccggca aaacctatga ctttgctgac gcaccaacca aactgctgac
     1561 caccgttcag gactgctggg taatgcatcc gggcgaaagc tggcacggct tcaaagatat
     1621 tccggataac tggagtatgc tcgacccgat taaagtcagc atccttgctc cgggaatggg
     1681 tgaagatggt gaactggaag aaaccggtgt tccggcggcg ctggtcactg cctggcttgg
     1741 tcgccacggc attgtgccta cccgcaccac tgacttccaa attatgttcc tgttctctat
     1801 gggcgtaacc cgtgggaaat gggggactct ggttaacacc ctttgctcct tcaaacgcca
     1861 ctatgacgcc aacacaccgc tggcgcaggt gatgccggaa cttgttgaac aatatcctga
     1921 cacttacgcg aacatgggga ttcacgatct gggtgacacc atgtttgcct ggctgaaaga
     1981 aaacaaccct ggcgcacggt tgaacgaagc ctattccggc ctgccggtgg cggaaatcac
     2041 cccgcgtgaa gcgtacaacg cgattgtcga caacaatgtc gaactggtat ccattgaaaa
     2101 tctgccagga cgtatcgcgg caaactcagt tatcccgtat ccgccaggaa tcccgatgct
     2161 gctgtctggt gaaaacttcg gcgataaaaa cagtccgcaa gtaagttatt tacgctcgct
     2221 gcaatcctgg gaccatcatt tccctggatt tgaacacgaa actgaaggga ctgaaattat
     2281 tgacggtatt taccacgtta tgtgtgtgaa agcgtaacca ctattccgct gaaggcgtaa
     2341 ttgtttaaat aacattacgc cgcctggcct taggcctttt gagtatggca acgttttcat
     2401 aaaaattgct gcaaacaaaa atgtcat
//
LOCUS       DAZFBR010000267         2384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFBR010000267 DAZFBR010000000
VERSION     DAZFBR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.270"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..809
                     /gene="espR4"
                     /locus_tag="ACHJZ2_005072"
     CDS             <1..809
                     /gene="espR4"
                     /locus_tag="ACHJZ2_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR4"
                     /protein_id="HFO4570984.1"
                     /translation="PIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGGS
                     NDLKENVDRALVRMFKCVKLHSNELDLSYLFLGSVPPLPDWIEMLSLVYNELDSIQVP
                     ESCKELELDFNNLTEFPQVPDGITLISVNNNLISHIDSFPPKAKKIFISHNKLSETPA
                     IPDTAKVFDCGYNKIQEIRYFPKNLKEARIGYNNIEVVPAIPGNLKILFMECNPIKEA
                     FLMPWTLTGICYEISQRKYIVTNPADYDKYSDMVKKHVIDGEELIIKYFM"
     gene            complement(947..1276)
                     /locus_tag="ACHJZ2_005073"
                     /pseudo
     CDS             complement(947..1276)
                     /locus_tag="ACHJZ2_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1234..1444)
                     /locus_tag="ACHJZ2_005074"
                     /pseudo
     CDS             complement(1234..1444)
                     /locus_tag="ACHJZ2_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140296.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1628..>2384
                     /gene="espR3"
                     /locus_tag="ACHJZ2_005075"
     CDS             1628..>2384
                     /gene="espR3"
                     /locus_tag="ACHJZ2_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR3"
                     /protein_id="HFO4570985.1"
                     /translation="MFPLNDLSLKTQSVQLNKITSNTESTIKQHELVSDDAIINELSS
                     ELVSCLGNDKFTPVSEDSNLLNMLSEFKLLREQCFRWGNYTLLFENYGAYDKTGSITI
                     EKSQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGASNDLKE
                     NVDRALVRMFKCVKLHSNELNLSSLSLGSVPPLPEWIEMLSLVYNELDSIQVPESCKE
                     LELDFNNLTEFPQVPDGITLISVNNNLISYIDSF"
BASE COUNT          745 a          438 c          491 g          710 t
ORIGIN      
        1 tacccattcg gcataaatta gagtttatat cgactaatat tgcagagttg ctggacaagt
       61 taacgaaaat tacagatgcc agactttgca aaggcttcag tgactgggct agttcagtca
      121 aagaaggcgg atcgaatgac ctcaaagaaa atgtagatag agcgttggtg agaatgttta
      181 aatgtgttaa gcttcacagt aatgaacttg acttatcata cctatttttg ggttctgtgc
      241 cgcctcttcc agattggatt gaaatgctta gccttgttta taatgaactt gattcaatac
      301 aggttcccga atcatgcaaa gaattagaac tcgatttcaa taaccttaca gaatttccac
      361 aagtacctga tggaattacc ctgatctccg taaataataa cctgatatcg catattgact
      421 catttccgcc aaaggctaag aaaattttta ttagtcacaa taagctatcg gaaacaccag
      481 caataccaga caccgctaag gtttttgatt gtggctataa taagatacaa gaaattcgat
      541 acttccccaa aaatctgaaa gaagcgcgta ttggatataa taatattgag gttgttcctg
      601 cgatacctgg caatttaaaa atacttttta tggaatgtaa tcctattaaa gaggcatttt
      661 taatgccatg gactctgaca gggatctgct atgaaatatc gcagcgaaaa tatattgtta
      721 cgaatccagc cgattatgat aaatattccg atatggttaa aaagcatgta atagatggtg
      781 aggaactcat aattaaatat tttatgtaat taattaggcg taattgtgtc actggaatag
      841 aggtttcttg tgacgcatgt ggaaggagcg tctatggaca aggcgtttcc tgctggtgat
      901 aaataaaggc gcagcacgtc gaatgcaaga atgcgctgcg tctggctcat ggcaattcat
      961 tatcttcgca tagcgtcaat gacaaaaatc gactgtgtgg atcaaattga taatcagcaa
     1021 acggttcgca atcaacgaaa ccatgcttca aataaagttg atggcaggcc gtaaatccag
     1081 cctgtgtacc cgtttctaag ctcaggcgat gaaggcatct gtcatgggcg acctgcaaaa
     1141 tgtggcgtaa aatcagactg gcgacacccc gacgtaaata atttggcgcg gtacgcattg
     1201 atttcagttc gccatgttta tcatccagca actttagcgc acccattcct gcgagttgtt
     1261 cgccttccca tactgacctg ttgacctggc ttgtttaatg acatccgatc tcaccgcgtg
     1321 gggttatgga tggggcaaac tcactcaatt tctggtttaa aatggaaacc tgattctgaa
     1381 agttttctgc cacccacgat ccgtatacgc gataaaccct ctgatcatca gaatgtctca
     1441 tttgacacat caccacgtcg gatgttgtta cgaccacaga cgcaacagta acctctggat
     1501 aacttaatct tcgttcttat ttttattaat atactgcgtt ataaaaatga gcgatttttg
     1561 atagtggcaa ttttattgtg ttttagaatg cgccctgggc aatataattt attaaacagg
     1621 agtgtaaatg tttccattaa atgatctgtc gttaaaaact cagtcggttc aattaaataa
     1681 aatcacatcg aatactgagt ctacgataaa acaacacgag ttagtatctg atgatgcaat
     1741 cataaatgaa ttatcaagtg agttagtcag ttgtcttgga aatgataagt ttacaccagt
     1801 tagtgaagac agcaacttac tgaatatgct gtctgaattt aagttattga gagagcaatg
     1861 tttcaggtgg ggtaattata ctctattgtt tgaaaattat ggggcttatg ataagacggg
     1921 atctatcacg atagaaaaaa gtcaggggga ggggacttta cccattcggc ataaattaga
     1981 gtttatatcg accaatattg cagagttgct ggacaagtta accaaaatta cagatgccag
     2041 gctttgcaaa ggtttcagtg actgggctag ttcagtcaaa gaaggcgcat cgaatgactt
     2101 gaaagaaaat gtggatagag cattggtgag aatgtttaaa tgtgttaagc ttcacagtaa
     2161 tgaacttaac ttatcaagcc tttctttggg ttctgtgccg cctcttcctg agtggattga
     2221 aatgcttagc cttgtttata atgaacttga ttcaatacag gtgcccgaat cgtgcaaaga
     2281 attagaactc gatttcaata accttacaga atttccacaa gtacctgatg gaattaccct
     2341 gatctccgta aataataacc tgatatcgta tattgactca tttc
//
LOCUS       DAZFBR010000268         2349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFBR010000268 DAZFBR010000000
VERSION     DAZFBR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.271"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..220)
                     /locus_tag="ACHJZ2_005076"
     CDS             complement(<1..220)
                     /locus_tag="ACHJZ2_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4570986.1"
                     /translation="MKIKHENIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKI"
     gene            complement(204..527)
                     /locus_tag="ACHJZ2_005077"
     CDS             complement(204..527)
                     /locus_tag="ACHJZ2_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4570987.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            652..1128
                     /gene="racR"
                     /locus_tag="ACHJZ2_005078"
                     /gene_synonym="rarC"
     CDS             652..1128
                     /gene="racR"
                     /locus_tag="ACHJZ2_005078"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFO4570988.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            1444..1596
                     /locus_tag="ACHJZ2_005079"
     CDS             1444..1596
                     /locus_tag="ACHJZ2_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4570989.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(1711..2226)
                     /locus_tag="ACHJZ2_005080"
     CDS             complement(1711..2226)
                     /locus_tag="ACHJZ2_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4570990.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
BASE COUNT          676 a          555 c          497 g          621 t
ORIGIN      
        1 gaattttttc aacagcatca ggggtgtctt tatccagcca acggaaaatt ttctgggtat
       61 tacgagccag ggcttccgga tggctgtcgt cgtacagttc cgggaacgtc atccccagtt
      121 cgaaataagt ccgggctatt tcagctgcag gaactttctc accatcagga tatgcccagg
      181 cattcatcgc catgcggatg ttctcatgtt tgattttcat gaatcatttg cctcttgatg
      241 cttcgggtat gatcgttttc gtcatttggt tgcttcatcg acatattctg cgaataacat
      301 gacgagcgtc gtaagtatgt ccaatcaaca tcaggacgaa gttcttcaca caggacacca
      361 ccttttgttg ctcgttcaat cgcaggacat ctctcagcag gcaactgacg tacacctttg
      421 atccattgat ttacgcttgg aggagataca cctaaaagcc tagccattgc tgattgccca
      481 ccgacaacag cacaagctcg tttgaatgaa tagttatctt ttttcatcga atgaactcca
      541 aaaaacacac aataatatta ggcttagcct aatgcaattg tcaataggct atgcctaacg
      601 cctcgagagt aggtattgcc taatgcgatg cacataggag actattaagc aatgcttagt
      661 ggtaaagact taggccgagc gatagagcag gccattaaca aaaaaattgc atcaggagcc
      721 gtcaaatcaa aggcggaaat cgcacgtcat ttcaaagtcc aaccaccatc aatccatgac
      781 tggattaaga aaggttcgat aagtaaagac aaacttccag aactatggcg tttcttttct
      841 gatgtggttg gtccagagca ttgggggctt aacgaatacc ccataccaac cccatccact
      901 tcagatacaa aaagtgaact tttagacata aacagccttt atcaagccgc ctctgatgaa
      961 aaaagagcaa ttgtggcttt cctcttatct ggaaatgcta cggagcctag ttgggttgat
     1021 catgacgttc gtgcctacat tgccgcaatg gaaatgaagg tagctaacta tctgaaaaat
     1081 caagaatcaa aacggaaaag ccagaacatc accaagacag gaacttaaac ttatatggtc
     1141 cgacgggaaa ttcctagttc ccgttagtta actcctacta cctctcccac aaaccatcac
     1201 ctattaggtc gcacccaaat cattaggcgt agcctattga caagcaatta ggcatttcct
     1261 atagttttcc cataccaacc catcccgtcc cacacaatac agggcaatac ctagagttac
     1321 ccggcagtgg tcagggatta agtagccagc ccgaggcgta agaacatgac ggcagggttc
     1381 aactttaact atgcagcagg tttttgttcc gctaccccgg cgttaagggg atatgaggtc
     1441 agcatggata ctcttgatct tggcaacaac gaatctctgg tgtgcggcgt gtttcccaac
     1501 caggacggca cgttcaccgc gatgacgtat accaaaagca aaacgtttaa aaccgaatct
     1561 ggcgcgcgtc gctggttagc aagaaactct gactaatgaa atctggtaat taaggaatcc
     1621 tccacggggg gagagtggtg cacacgcgcc ggacacaagc aagcatccgg catgctcttt
     1681 aacaatctgg atatccataa cagtaaaaat ctacagattg ccgttcagtt ttctggccaa
     1741 ctcttcaata gatggaggtg atacgtaatc cggattttta ttcataagaa actgattttc
     1801 acagtacagg cacctgcttt tatgaaaaaa ttcatattct ctaaccggga atggctgaag
     1861 tatcgatact atcttttgtc caaaacattt tggacaaaga tgcatgatta tgctgccgcc
     1921 gttcacggtt acctccttcg agtacacaaa agttcccgac tcaagttggc taaggatata
     1981 accttccgtc tgagcctcaa agttttcaaa ttctgcaatt ttagctttga gagaagcatt
     2041 tatttcttga taagtgccca ccagctcaac gagagacacg cattcgcgct gaatagatgc
     2101 aagctttgag ttcagttcac caatagccgc atttacttca gcttgagttt ttgcctcgtt
     2161 cattagtttt gcaatctggg cggtttcacg aatagccgtc attgctgccg ttaattcagc
     2221 gatcacattc aatactctta ttgttgttgg ggatatccag attaacaaga tccttgttgt
     2281 tggggaataa cggggaccac ctcgcctgac gtggtaaaaa gcaggcacac aacacgaaag
     2341 cacacggcg
//
LOCUS       DAZFBR010000269         2317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFBR010000269 DAZFBR010000000
VERSION     DAZFBR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.272"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..132
                     /locus_tag="ACHJZ2_005081"
     CDS             <1..132
                     /locus_tag="ACHJZ2_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4570991.1"
                     /translation="VIIKSTSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(143..214)
                     /locus_tag="ACHJZ2_005082"
                     /pseudo
     CDS             complement(143..214)
                     /locus_tag="ACHJZ2_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            299..382
                     /locus_tag="ACHJZ2_005083"
                     /pseudo
     CDS             299..382
                     /locus_tag="ACHJZ2_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(503..1516)
                     /gene="nleC"
                     /locus_tag="ACHJZ2_005084"
     CDS             complement(503..1516)
                     /gene="nleC"
                     /locus_tag="ACHJZ2_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO4570992.1"
                     /translation="MYKTILSINMKIPVLQPGFNFFAPAGYSAAVAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEEVHIGCIKYRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
     gene            complement(1919..2188)
                     /locus_tag="ACHJZ2_005085"
     CDS             complement(1919..2188)
                     /locus_tag="ACHJZ2_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4570993.1"
                     /translation="MNILKKLMQRLCGCGKHDDREHGELLTAQLRLGPADILESDENG
                     IIPEQDGVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(2190..>2317)
                     /locus_tag="ACHJZ2_005086"
     CDS             complement(2190..>2317)
                     /locus_tag="ACHJZ2_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4570994.1"
                     /translation="PKDATRVQGFFRHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          618 a          594 c          450 g          655 t
ORIGIN      
        1 gtgatcatca aatccaccag caacgatggc ctttcagtta ctcgcaccgc cgataaagat
       61 tctgcaaaaa cttttgttca ggagacgctg aaagatacct gggagtctgc tgacgagtgg
      121 tttgttcgct aataaacacg taaatcggta acggctggaa atcattcaat actcgcacta
      181 tcgaaagttc accagccaac cgcgacacgc tcttacatac gaagtgccgc gctttcctta
      241 ataatttttt agcagtactg tgtaaatgat gagcgaccta atccatgcat gacgcgtagt
      301 gcctattgtg catctttctg cgtctctttt tactggaccc aagccaatga tgctggcgat
      361 accattcccg ccaggcccac ttacaacagt aaaattcgga gctctgcctt tacatacaat
      421 acaatgttgc acgattgccc atcataaact ccgttaatat ctatgagtag taaccctatg
      481 taactgtaaa gacgaatcag cgctaattgt gtctgtccac atccccaaat gtaaagatat
      541 aaggagcacc atgatacagt tggatctgat ttacatcctg taaattctcg tcgatagcca
      601 cccctggaaa atcatgttgc tggataaatc cgtatcctat gttagacaca gcggaatact
      661 ctgtgaaatc aggactcgcc tcatatcggt cactgatcgt acccagtctt tcgaagaaag
      721 ccctctcatt cttttcatgc ctcatggcag cctgtcgaag ggcattcagg ttacgtagtc
      781 taagatgagc ttctcgttct ggctctgcat atcctttgaa gtcgggaaca ctccatccca
      841 gctcttgagc gacacgacgt gcgagaatct cggtgggtcc tagctctata ttactatctc
      901 cagatggatc gctggcccca gtaacatgat gaataatctc gtgaatcagt ccttcctgcc
      961 acgatggcat ctcataagaa tcagtatctg gcgcaacact aaaactgaca taaggctctt
     1021 cgttctcgtt ttctcctgcc tcgcaaatgg ggagaattgg ttcttgcccg acatcgtatc
     1081 catataattc gttacaggtt agtgattgaa tatcatcaat tttgatagca gattctccgt
     1141 taagctcgta ttcgtttctg tatttaatgc aaccaatgtg tacctcctca ttatgaatgc
     1201 catagcttac ggcatcacga aatgtttgtg atcggctcaa cgcatcaagt acagtgttgc
     1261 caatcatatc gaccgtatgc tgatcgatga gcctgctacg gctgtcatgg acggcgcgaa
     1321 ttacactttc gtatacgttg cttaaatctg ctgcggaaag cggtattcgc ttacctatat
     1381 ccaaaacgta atccgcatag gcattttcag cacgattagg agcaacggca gcagagtatc
     1441 cagcaggggc aaaaaagttg aagccaggtt ggagaacggg aattttcata ttgatactta
     1501 atatggtttt atatatttca gtgccaccaa ttcactcgct tacaaccaga aactaaaaaa
     1561 acgtcagggc gttgataata atgaatacca actttctgcc catttatagt aatatctttc
     1621 gttatcaggt tttgtctggc agcttcacgt aatatttcaa tactctgcaa agtaacattt
     1681 gatgaaatgc tggatgttat caatggcata tgtcatttac cccaacaaga ctaaatttta
     1741 gaaaaaacaa ctgccgggac aactttcgcc ccctgtaacc ccaaataaga tgtcagattt
     1801 atcggcatta atttatctcc ctgttgaatt agcacctcta tcatctactt aacaaatatc
     1861 ccacggacat tgcaacacaa aaaccggagc cggactccgg ttttgtgaag ctgtcgggtt
     1921 acttcatccc gccaatattt tcccacgtcc cgtcagcacg caggatttgc agcggtctta
     1981 ccacgcactg tatctgcttt ttatccgcat ccagtatcac cacctgcgtg attaccccgt
     2041 cctgctccgg gataatacca ttctcatctg actccaggat gtctgccggc cccagtcgca
     2101 gctgtgctgt aagtaactcc ccgtgttcac ggtcatcatg ctttccgcaa ccgcacagac
     2161 gctgcataag tttttttagt atattcatgt cattctcctg ttctgcctgt atcactgccc
     2221 acttcatcca gtcccttaac atcctgccac ggcccgtcac caaacctgac ctgcaaatgc
     2281 cgaaaaaaac cctgaacccg tgtggcatct ttggggt
//
LOCUS       DAZFBR010000270         2317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFBR010000270 DAZFBR010000000
VERSION     DAZFBR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.273"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            83..904
                     /locus_tag="ACHJZ2_005087"
     CDS             83..904
                     /locus_tag="ACHJZ2_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4570995.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEITDSLLMADLT
                     VRQLKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAKSANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GDGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            901..2276
                     /locus_tag="ACHJZ2_005088"
                     /pseudo
     CDS             901..2276
                     /locus_tag="ACHJZ2_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
BASE COUNT          648 a          539 c          641 g          489 t
ORIGIN      
        1 ttagtggtag ccgcagcaaa taacagggcg ttcgcagagc gccttgttgg tgtttacaga
       61 ctagccaaag caggagtgaa acatgggcgt cgttaagtta gctgattaca ggcctcaact
      121 ggaggtcgtg gagcatcgcg tggcagatac cgaagatggt ttcatgcgcg ttgctaacga
      181 gattaccgac agtctgctga tggctgattt aaccgtccgg cagttgaagg tgatgctcgc
      241 tatcatgcgc aagacatacg gattcaataa gccgatggat cgactcacaa acacgcagat
      301 agcagccatg acaggtattc atcacactca tgtttgcgct gccaagcgcc agcttattga
      361 gcgtaaattc ctcattgctg atggcgtgaa aatcggagtg aacaaggtgg tttctcagtg
      421 gattagccag gacagcttaa cattagctaa aacagctaat aaaacattag ccaagtcggc
      481 taatgggtat aagccaagtc agctaaacac aaaagacaat atacaaaaga caataaatac
      541 aaataccccc ttacccccta acggggatgg cgatgggcag gttaaacctg aacgtcgcaa
      601 ggcagaacga atcgactacg aatccttcct gaacgcctac aacaccgaag tcggtgacag
      661 acttccacac gctgttgcgg tcaacgagaa acgaaaacgc cgcctgaaga aaattatccc
      721 gcaactgaaa acgccaaacg tggacggttt cagagcgtat gtcagggcgt ttgtgcatca
      781 ggccaagccg ttttacttcg gagacaacga cacgggctgg acggcagatt ttgattacct
      841 gctgagggaa gattcgttaa cgggagttcg ggaagggaag tttgcagaca gggggattgc
      901 atgagacagg atatcgaagc tagcgttatc ggtggcctgc tgattggtgg attaactcca
      961 accgccagtg acgttctggc aacgctggag ccggaagcgt tttcaattcc gctctaccgg
     1021 aaagccttcg aggttattcg caagcaggcg agaaacagaa acctaatcga tgcgctgatg
     1081 gttgccgagg cgtgcggaga ggagcatttc acgtcaatcc tgatgaccag caaaaactgc
     1141 ccgagtgccg caaacctgaa gggatatgcc ggaatggtcg cggataacta tcaccgccgt
     1201 ctggtgctgg aaatcatgga tgaaatgcgt gaaccaatcc aaagcggaac catcgacgca
     1261 tcgagtcagg cgatggatga acttgtaaag cgtctttcag ccatcagaaa gccccgtgac
     1321 gaggttaaac ctgtacggtt aggggaaata tcactgacta cactgacacg cttgacaggc
     1381 gtctgaggaa cggagaagag tcagataccc tgaagaccgg aatcgaagag cttgacgcta
     1441 tcactggagg gatgaacgca gaagacctgg tgattatcgc cgctcgtcct ggtatgggga
     1501 aaaccgaact ggcgctgaag attgccgaag gtgtggcaag ccgcgttatt cctggttctg
     1561 acgtccggcg cggggtattg attttctcaa tggaaatgag cgcattgcag attgcagagc
     1621 gaagcattgc caacgccggg aggatgtcgg ttagcgtact gcgaaatcct gcatcgatgg
     1681 atgacgaggg ctgggcacgt gttgctaacg gcatgagtca gcttgcagat ttggatgtat
     1741 gggtagtcga tgcttcgcgg ttatcggtcg aagaaatacg ctcaatcgca gaacggcata
     1801 agcaggaaaa tccaaacctg tcactcatca tggcggatta tcttggcctg attgagaagc
     1861 cgaaagcaga ccgcaacgac ctcgcaattg ctcacatctc cggaagcctg aaggcgatgg
     1921 cgaaagacct gaaaacaccg gttatctccc tgagtcagct ttcgcgcgat gttgagaagc
     1981 gaccaaacaa acgcccgaca aacgcagatt tgcgtgattc aggaagcatt gaacaggacg
     2041 cagactcaat catcatgctc tatcgggaag cggtatatga tgagaacagt agcgccgcgc
     2101 catttgctga aatcatcgtg acgaaaaacc gttttggctc gcttggtaca gtttaccagc
     2161 ggttctgtaa cggacacttt gttgcatgtg accaggatga agccagacag atttgcacag
     2221 catcaaatgc acctgctgcg cgtggcagac gatatgcaca aggggctgac gtatgaccat
     2281 ctacatcact gagctaatag cagggttatc gttacta
//
LOCUS       DAZFBR010000271         2294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFBR010000271 DAZFBR010000000
VERSION     DAZFBR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.274"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>2294
                     /locus_tag="ACHJZ2_005089"
     CDS             <1..>2294
                     /locus_tag="ACHJZ2_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFO4570996.1"
                     /translation="SAIKLFSRNDADTKIIVWYNSVMEKNVFLREVISCVLRSKKVDS
                     YINENKKNLSKEDAGALRDYAKLKMKELFSMLDDDGYKKIITTNAYIKERDKLSGIIY
                     NIENSIISGHESFDIIRSNQHEWGDLSTVEQFKKFEFYVKSELSSAKSIFDDIKNKYI
                     TDPETKRNVLYHQLDSDIKERIAFLDISHYAYPGSLLEKLQLSGYVFSDINIIAEYLL
                     ASYGVSGHYSHGVVYPAPSDKLLELLRRHTKSNSEWIEKITPYVYDILSDNVSNVLRP
                     PLSEEQKKILNDIKLEISKSVSEQYFMKLTEQKSSVIGIKYSVDFDRYNENLFLSLPI
                     NQNLTLPFMYRYFEMLYDIHIGIIENKANREFIYSKFSSLNLDFLINDERVLNLEGLI
                     KKYKYLSLSEIHRTLTNSTSFADISIPLLQTICPSITTIIKKTEYYGHQLTNAMTVAS
                     VVKPYDFSNLGAINSIDKSVSDVPALHTIVEQAKYNLLSWNDFYNTHASIWDTIARQH
                     KSTNIEFHPQSLLFDRDSKGKCLGLSLLYLDTGGYGGGYQKLRHNIDTASTLYQTKYN
                     DNLKLSNRDDFFLRKTQRIITMSNELGNNRLKNAQLEVLELKDPILTEGILYQRRISS
                     LLITTEYHSLALQQISSFWRVTDPNFGHCDFHSLAQALTFIKNITSNRNFSSLYGSGI
                     VKIYFSESLNNWKYIKLPLVQTGSLLRDIYLTTPEKLSTSGGSLNIMGHLVPVSFIYD
                     IGGVINGNRISESTDVKNKIRSLK"
BASE COUNT          847 a          340 c          387 g          720 t
ORIGIN      
        1 cagcgcaata aaattatttt cgcgtaatga tgccgataca aaaattattg tctggtacaa
       61 ttccgtaatg gaaaaaaatg tgtttctccg agaggttata tcatgtgttc tacgaagtaa
      121 aaaagtagat agttatataa atgaaaacaa aaaaaactta tctaaggagg atgccggagc
      181 tttaagagat tatgcaaaat taaaaatgaa agaactattt tcgatgttag atgatgatgg
      241 ttataaaaaa atcataacta caaatgcata tattaaagag agggataaac tatcaggcat
      301 tatatacaac atcgaaaatt cgattatatc gggtcatgaa tcatttgata taatacgaag
      361 caatcaacat gagtgggggg atttatcaac ggtagagcaa tttaaaaaat ttgagtttta
      421 tgtgaaaagt gaattatctt cggctaagtc aattttcgat gatataaaaa acaaatatat
      481 aacagatcct gaaacaaaac gaaacgtact ctaccatcag ttagatagtg atattaaaga
      541 gcgaatagca tttctcgata tatcccacta tgcatatccg ggtagtttat tagaaaaatt
      601 acaattaagt ggctacgttt tttctgatat caacataatt gcagaatact tattggctag
      661 ttacggagtt tctggacatt attctcacgg cgtggtatat ccagctccat ctgataaatt
      721 acttgagcta ttaagaagac atacaaagag caattctgag tggatagaaa aaataacccc
      781 atatgtttat gatatattat ccgataatgt atccaacgtc ttgcgccccc ctttatcaga
      841 agaacagaaa aaaatattga atgatataaa attagaaatt agtaaatctg tatcagaaca
      901 gtatttcatg aagttgacag agcaaaaatc atcagttatt ggtattaaat attctgttga
      961 ttttgacaga tacaatgaga atttgttttt gtctttacct ataaatcaaa atttaacgct
     1021 gccttttatg tatcgctact tcgagatgct atatgacatt cacattggaa taatagaaaa
     1081 caaggcaaac agggagttta tatatagtaa gttttcatct ttaaacctgg attttttgat
     1141 taatgacgag agagttttga atcttgaagg tttaataaaa aaatataaat atttatcgct
     1201 gagtgaaata catagaactt taacaaatag tacttcattt gcagatattt ctataccatt
     1261 attgcagacc atatgtccca gcataacaac aataataaaa aaaacagaat attatgggca
     1321 tcagttaaca aatgctatga ctgttgcttc agtggttaaa ccatatgact ttagcaatct
     1381 tggtgccata aatagtatag ataaatccgt ttctgatgtg cctgcattgc atacaattgt
     1441 tgaacaagca aaatataatt tactttcatg gaatgatttt tataacactc atgcgtcaat
     1501 atgggataca atagcaagac agcataaaag tacaaatatc gaatttcacc cacaaagtct
     1561 tctttttgat agagattcga aaggaaagtg ccttggttta tctcttctgt atcttgatac
     1621 aggggggtac ggaggcggat atcaaaaact gaggcacaat attgacactg ccagcacatt
     1681 atatcaaaca aaatacaatg acaatctaaa attatctaac cgtgatgatt tctttttaag
     1741 aaaaacacag cgtatcatta caatgtccaa tgagttaggt aataacaggt taaaaaatgc
     1801 acagttagag gttctggaat tgaaagaccc tatactaact gaaggcatat tataccagcg
     1861 tcgtatatca tctcttttaa taacaacaga atatcattca cttgcattac aacaaatatc
     1921 ttctttttgg agagtaactg atcctaattt tggtcattgt gactttcatt ccttagcaca
     1981 ggctttaacg tttataaaaa atataacgag caatcgtaac ttttcatctt tatatggaag
     2041 tggcattgtt aaaatttact tcagtgaatc tcttaataat tggaagtata ttaagctccc
     2101 ccttgttcag actggttctt tgttaagaga catatattta acaaccccag aaaaattatc
     2161 cacaagcggg ggttcattaa atattatggg gcatctcgtg cctgtcagtt ttatttatga
     2221 tatcggaggg gttattaatg gtaacagaat aagtgaaagt acagatgtga aaaataaaat
     2281 taggagctta aaaa
//
LOCUS       DAZFBR010000272         2274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFBR010000272 DAZFBR010000000
VERSION     DAZFBR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.275"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            297..458
                     /locus_tag="ACHJZ2_005090"
                     /pseudo
     CDS             297..458
                     /locus_tag="ACHJZ2_005090"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..507
                     /locus_tag="ACHJZ2_005091"
     CDS             442..507
                     /locus_tag="ACHJZ2_005091"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFO4570997.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            623..880
                     /locus_tag="ACHJZ2_005092"
     CDS             623..880
                     /locus_tag="ACHJZ2_005092"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4570998.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(927..1037)
                     /locus_tag="ACHJZ2_005093"
                     /pseudo
     CDS             complement(927..1037)
                     /locus_tag="ACHJZ2_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
     gene            1048..1965
                     /locus_tag="ACHJZ2_005094"
                     /pseudo
     CDS             1048..1965
                     /locus_tag="ACHJZ2_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          706 a          503 c          514 g          551 t
ORIGIN      
        1 aaaaacaact ttttatcttt ttgtttcgct caaattagta taaaaagccg aacaacaaac
       61 aataaaaaac cattaacatc aatagattat gatggcttca atctaaataa taggctatat
      121 aatactgcaa gacacaacac atgctgtcac tatgagtcaa ctatttagat gatattagtg
      181 acctgtttca gagcattagc gcaaggcgat tctttgtctt cttgcactaa ttttttatca
      241 taaaaatgtt cctagcactg ggcatcaata tcgcaggtca gaaagagctc ctggggatgc
      301 ggctggccga aaatgaaggg gcgaatttct ggttcaatgt gctgactgaa ctgaaaaacc
      361 gcggtctgaa cgatatcctc atcgcctgtg tgtatggcct gaaagaattc ccggaggccc
      421 gcatccagtt atgcatcgtg catatggtgc gcaacagcat gcgcttcgtg tcatggaagg
      481 aatacaaagc cgtcactcgc gacctgaaag cgattagcct cccacagaag aggcaggcca
      541 gcaggcactg gaagcgtttg ctgcggcctg ggactgccgc tatccgcaga taagccggtg
      601 ctagctgtca aactggacta acttggcgac gtttttcgct tatccggcag atatccgcaa
      661 agtgatctac acaacgaacg ccatcgagtc gctgaacagt gtgatccggc atgccatcag
      721 gaaacgcaag gtgttcccga cggacgagtc agtaaaaaaa gtggtgtggc tggcaatcca
      781 ggccgcgtca cagaaatgga caatgccgtt aagggactgg cgaatggcaa tgagccgctt
      841 tattatcgag tttggtgacc gtcttgacgg tcacttttaa gaaaaggcat ttacacagaa
      901 tcctaaacag gctcacaggc ctctaatcat ccacattgta aagatccttt gttgtaagta
      961 agatctggta ccctaataat atcaaaagga ttaaaatcat cactgcatcc ttccacgagg
     1021 catttcattg ctcgtagaat tcacaccaac attgagcttt tcagcgaaag tgggcacgaa
     1081 tcattgctgg acagggttaa aggcactgac tcaatcactc ttcatacgtg gcttgatcga
     1141 tatgaaacaa tcctcagcta gagggggagc aagccgaaaa cattactcga ctacgccagc
     1201 aaaatcaggg caattcgaag aaaattgccg gacaaaccgc tcactgacat atcaacgaaa
     1261 gaggtggcag caatgctaaa cacctacgtc gcagaaggta aagcggtttc cgcaagagta
     1321 atcaggtcaa cccttgttga cgtttttcga ggggcaatag ccgaggggca tgtggcaacg
     1381 aatccagtaa caacaacccg tgcagcaaag tcagaagtaa ggcgctcaag gctgacagct
     1441 aatgagtatg tcgctattta ccatgctgct gagcacctcc ccatctggct gaggctgtca
     1501 atggatttag ctgtcgttac agggcagaga gtgggcgatt tgtgcagaat gaagtggtca
     1561 gacataaacg atggtcatct tcacattgga cagagtaaaa caggagccaa aattgccatt
     1621 ccgctggctc taaccattga cgcactcgac atctcactgg ttgatacact acagaaatgc
     1681 agggaggcca gcagcagtga aacaataatc gcatcaacct atcacgaacc actttctcca
     1741 gccacagtat cacggtattt aacaaaggcg cgaaatgcat ccgggatctc gtttgatgga
     1801 gacccaccga catttcatga actacgtagt ctgtccgcga ggctatatcg gaaccagatt
     1861 ggctacaagt ttgcacaacg tcttcttgga cataaatctg attcaatggc ggcgcattat
     1921 agggacagtc gcgggcggga gtgggacaaa attgaaatcg gataatgatt ttattttgac
     1981 caataatgac ttaccagatt taacaacttg atatttaata agatttttga acgaacaact
     2041 tccatgtcag aaggaggata aaatcacaaa aacacattaa aaatcattat gttatttata
     2101 caattgatta aatgatagac tgcaaaacgc gacaaaacac tacatttaga gtcacaatta
     2161 atcaatgagt tagatgtgat tagtgacctg agacagagca ttagcgcaag gtgatttttt
     2221 gtcctcttgc gctaattttt tgtcatcaaa catacagcca atcagaagag cata
//
LOCUS       DAZFBR010000273         2248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFBR010000273 DAZFBR010000000
VERSION     DAZFBR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.276"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..1271)
                     /locus_tag="ACHJZ2_005095"
     CDS             complement(<1..1271)
                     /locus_tag="ACHJZ2_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4570999.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEYYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLI"
     gene            complement(1246..1791)
                     /locus_tag="ACHJZ2_005096"
     CDS             complement(1246..1791)
                     /locus_tag="ACHJZ2_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4571000.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
     gene            2178..>2248
                     /locus_tag="ACHJZ2_005097"
     CDS             2178..>2248
                     /locus_tag="ACHJZ2_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571001.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIE"
BASE COUNT          566 a          633 c          515 g          534 t
ORIGIN      
        1 tcaatcagcc agctttcctc acccggcccc catccccata cgcgcatttc gtagcggtcc
       61 agctgggagt cgataccggc ggtcaggtaa gccacacgat caggaacggg cgctgaataa
      121 tactctttcc gctctgccat cacttcagca tccggacgtt cgccgatttt cgcttcccac
      181 gtctcaccga gcgtggtgtt cacgaaggtt ttacgttttc ccgtatcccc tttcgtcttc
      241 atccagtctt tgacaatctg cacccaggtg gtgaacgggc tgtacgccgt ccagatgtga
      301 aaggtcacac tgtcaggcgg ttcaatctct tcaccggatg acgaaaacca gagaatgcca
      361 tcacgggtcc agatcccggt cttttcgcag atataacggg catcagtaaa gtccagctcc
      421 tgctggcgga tgacgcaggc attatgctcg cagagataaa acacgctgga ggggtcatcc
      481 ggcgtccatt tgaggccaaa cggcgtctct ttgtcgccaa atttaagata ctgctcctcc
      541 ccgcagtgcg ggcaggcaac atgaaaacgc ataaaatgcg gggattcact ggctgcacgc
      601 tcaatctggc aggtgcctct cactttgggc gtggagccac ggatggactt tggccagacc
      661 gagccttcaa tacgcttgtc acccaggaac gtcggagagc cttcctgttc aatatcctca
      721 tcaaaggcag caagttcatc ataacccgcc acatcaaccg acttttcacg gtagtttttt
      781 gccgctttac cgcccaggca ccagaagcca cgaccattgg tgaaacgctt catagtgagc
      841 gtgttatccc ggtgcttttt gccataccac ggggccagag acagcagcga cggaatatcg
      901 cggatggtag gctcgacgtg ggttttcata aagttctcgg catcaccatc cgtcggcaac
      961 cagataaggg tgttacgctg cttatgctct atgaagtagg cataaacacc cagcagcatt
     1021 tttgaataac caacacgggc agacttcacc acattcacct cgcggatgta gtcgctgccc
     1081 atcgcattca tgatagcccg ctgaaagggt agtgtttccc agcgcccttc ctggtatgcg
     1141 gattctttcg ggagatagta actggcatcc gcccattcaa cggcagtctg tggctccggc
     1201 ctgaacaggg ctcgcagccc ggcgcgtaca tcacgccgca gaatatcaat ctgactgttc
     1261 gatatattca ctcagcaacc ccggtatcag ttcatccagc gcggctgctt tgttcatggc
     1321 tttgatgata tcccgtttca ggaaatcaac atgtcggttt tccagttccg gaaaacgccg
     1381 ctgtactgag agagggatcc cgtcaagaat actggcaatt tcacctgcga tccgtgacag
     1441 cacgaaagta cagaatgcgg tttccaccac ttctgcggag tctctggcat tcttcagctc
     1501 ctgggcgtca gcctgcgcac gcgtaagtcg atggcgttcg tactcaatag tcccaggctg
     1561 gagatctgcc tcgctggcag ccctgtaatc ctcaacctct ttacggagtt tttcattttc
     1621 gatatcagct tccctctgcg cataccactg aattgccgtg gcggtatcaa atacagattc
     1681 aacgccctta ccacctccgg agacgcaagg gagcccctga gactgccagc gttcaatcgt
     1741 tcgcggatcc acgttgaaaa tttcggcaag cttcttttta ttaaccttca tgaaacagtc
     1801 tcacaacaaa tacagggtcc gacatgaaag tgcccgaaaa tgactttttc aggcgttttc
     1861 atgtcggacc tttacggatt cgatattaga aaaaacaaat agttatgttc gagaagtacc
     1921 gacatgattt tccccggaaa attttcataa atagcgaaaa cccgcgaggt cgccgccccg
     1981 taaccggtcg gatcgccgga aaggacccgc aaaatgataa taattatcat ctacatgtca
     2041 caacgtgcat ctacgccatc aaaccacgtc aaataatcaa ttatgacgca ggtatcgtat
     2101 taattgatct gcatcaaatt aacgtaaaag caacttcaga taatacaaat cagcaacact
     2161 gaatatgggg caacattatg tcatcaaaga acagaacccg cagaacaaca acccgcaaca
     2221 tccgatttcc aaaccagatg attgaaca
//
LOCUS       DAZFBR010000274         2238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFBR010000274 DAZFBR010000000
VERSION     DAZFBR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.277"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..286
                     /locus_tag="ACHJZ2_005098"
     CDS             <1..286
                     /locus_tag="ACHJZ2_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4571002.1"
                     /translation="RPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGT
                     KSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
     gene            299..673
                     /locus_tag="ACHJZ2_005099"
     CDS             299..673
                     /locus_tag="ACHJZ2_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4571003.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGK
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            670..1491
                     /locus_tag="ACHJZ2_005100"
     CDS             670..1491
                     /locus_tag="ACHJZ2_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4571004.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1718..1915
                     /locus_tag="ACHJZ2_005101"
     CDS             1718..1915
                     /locus_tag="ACHJZ2_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4571005.1"
                     /translation="MLKQQDMTETARAVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            2066..>2238
                     /locus_tag="ACHJZ2_005102"
     CDS             2066..>2238
                     /locus_tag="ACHJZ2_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4571006.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYL"
BASE COUNT          591 a          478 c          623 g          546 t
ORIGIN      
        1 acgacctaaa cgtcgccgct gggtaaatga gaaatacaca cgctgggtaa agacacagcc
       61 gtgtgcgtgt tgtggtaagc cagccgacga tccccatcac ctgattggtc atggtcaggg
      121 cggaatgggg acaaaatctc acgatatttt cacgctaccg ctgtgtcggg agcatcacaa
      181 cgagcttcat gcggatccgc tggcgttcga agaaaagcat ggttctcagg ttgatttaat
      241 ttttcgtttt cttgatcacg cctttgcaac tggcgtgctt gggtaaaaga ggtgactgat
      301 gctcatagat ttggttttac cttacccgcc gacggtgaac acttactggc gacgccgtgg
      361 cagcacatat tttgtatcaa aagccgggga gcgttatcgc cgggcagtgg cgcttattgt
      421 tcgccagcag cgactgaaat taagcctgtc cggacggctg gcaataaaaa ttattgcaga
      481 gccaccggat aagcgccgcc gtgacctgga caatattctg aaagcaccgc tggatgcgct
      541 gacgcacgcg gggttgttaa tggacgatga gcagtttgat gaaatcaata ttgtacgtgg
      601 aaagccagta tctggtggac gtctgggggt gaagatttac cccataatgc ttgaagggca
      661 ggtcaaaaaa tgaaactgga agatttaccg aaatactact ccccaaaatc ccctggcctg
      721 accgatgcat cggcctcaac gtcaaaagat gcgctgagta tcactgatgt gatggccgcg
      781 cagggcatga cacagaatcg ggctgagatg gggttttctg cgttccttgg gaaaatgggc
      841 attagtatga atgacagaga gcgggcaaca gaattgctga cagaatatgc actcagtcgg
      901 tgtgatcgcg tggcggcgtt aagaaaactc ccggcagaaa taaaaccggc agtgatgcgt
      961 attatggctt cgtatgcgtt tgaagattat gcccgtagcg cggcgagcaa aaaacagtgc
     1021 ccctgctgtc acggaaaaaa atttattgaa agcgaggttt ttacaaacaa gatccagtat
     1081 ccggatggta agccgccagt gtgggcaaag tgcacaaaag gcgtgtatcc gtcttactgg
     1141 gaggaatgga aaaaagtcag ggaggtggta aaagttgcct gtccggagtg tggcggaaag
     1201 ggtgaggttt ccaccgcctg taaggattgc cgtgggcgtg gtgtcgccat tcatcgtgaa
     1261 gagtcggtaa aacgtggtat gcctgttatc agagactgcc agcgttgtgg tggtcgtggc
     1321 tgtgaaagac taccatcaac ggaggcattt aatgccatat gtaatgtaac cgatgccata
     1381 tctcttgata catggaaaaa aacagttaaa cgtttttacg atacgctggt ggtgcagttt
     1441 gatattgaag aagcatgggc agaacaacaa ctgaaaaagg tgaccagata gctttgttga
     1501 tttttcccga atctgtggta aatttgccct aacgatgggc gttttatgcc tgacgttaga
     1561 agatttttta caccccgccg cctggcgggt tttttatgac tgaaatcgcg tcagtacagt
     1621 aaacgcgctg gtggcggtga ataccggtct ttcagcttgc tggctttttt gacaagagtt
     1681 attggtgtgt cacgttaacc gaaaaaggga aaaagacatg ctgaaacagc aggatatgac
     1741 agaaaccgcc agagcagtgt ttaatgaatt aagcgtcacc gaaccggcga cagtcgggga
     1801 gattgcgcag aatacttacc tttcacgcga acgctgccag ttaatactga cccagcttgt
     1861 tatggcgggt ctggcagact atcagttcgg ttgttacaga cgccttcagt cctgaaggct
     1921 tttttatttg tggtaaatgg gcggctggtg ggtgttaggg gcactcacca gccatctgct
     1981 catgcgtctg gatcacaagc aaacctcagg cccactgctt tgcgcaaaag cagaatgagc
     2041 ctatcagaga caggcttaat gatccatgtt taacactgta aaaatatcca gttgtgagtt
     2101 gatcaacgct gactgcctgg aatttatccg gtcgttaccc gaaaattctg ttgacctgat
     2161 agtcacggac ccgccgtact ttaaagtgaa gcctgagggc tgggataacc agtggaaggg
     2221 cgacgatgat tacctgaa
//
LOCUS       DAZFBR010000275         2227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFBR010000275 DAZFBR010000000
VERSION     DAZFBR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.278"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1901
                     /gene="espN"
                     /locus_tag="ACHJZ2_005103"
     CDS             <1..1901
                     /gene="espN"
                     /locus_tag="ACHJZ2_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HFO4571007.1"
                     /translation="KKMRVISNNEMKKSIKISELVIHNIIKKGLTNCLLKKDVLNARY
                     DLIRDILRYSLNIRQGIKHDDVNRIAENIIKKYGITEGMNPKPRNARISKELLLLAVD
                     RQIEWAKKHFITKDVLENVVSKCDLSSIFNVNKVLQNTILEFVHEINNISSARWMSKS
                     EKNNKQKEAIEKFKKEVSHMNGGQQFIWGFDKVIQEGLSGLIELSIDINDSTNHRDKS
                     SLSPDGRAVLHFLGTIWNMAMGAVPGYNALSGVSSILHSAIVKESSNICDYIQGAVRI
                     GMDFVPGTRSDLHSRSLQIKYEALKHIEKNINDNIIYHPSNNANFYSVIESIDGNDFI
                     YNEKQSKILEMKQDRGGNRYSAVDLNSSKYGYYEKVGGGFYRYIESFNPISSETPNKI
                     VYKGESVDLTKEPNSELYSGRYSINNKQVNVYFFRDADGTFYKSEGLHGGGVIRYIDK
                     PYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIE
                     LSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYT
                     SDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAIIDRTFGMKEN
                     ISMVNASK"
BASE COUNT          755 a          322 c          467 g          683 t
ORIGIN      
        1 gcaaaaaaat gagagttatt tccaataatg agatgaaaaa aagcattaaa atatctgaat
       61 tagttatcca taatattata aagaaaggat tgactaattg tttgcttaaa aaggatgttc
      121 ttaatgccag atatgatctt attagagata ttcttcgata ttctttaaat atacgacagg
      181 gaattaaaca tgatgatgtt aatagaatag cggaaaatat aataaaaaag tatggtataa
      241 ctgagggtat gaatcctaaa cctaggaatg ccagaatatc taaagaattg cttttattag
      301 ctgttgatag acagattgag tgggcgaaaa aacattttat aacaaaagat gtattggaaa
      361 atgttgtgtc aaaatgtgat ttatcatcta tctttaatgt taataaagtg cttcagaata
      421 ctattcttga gtttgtccat gaaattaata atatatcatc tgctcgctgg atgtcaaaat
      481 cagaaaagaa taataaacaa aaagaggcaa tagaaaagtt caaaaaagaa gtatcccata
      541 tgaatggcgg gcagcagttt atttgggggt ttgataaggt tattcaagaa ggcttaagtg
      601 gattgattga gttaagtatc gatattaatg atagtacaaa tcatcgtgat aagtcttctc
      661 tttctcctga tgggagagct gtgttacatt ttttaggtac aatttggaat atggcgatgg
      721 gagctgtacc tggttataat gcattgtctg gtgtaagtag cattttacat agtgctatag
      781 taaaagaatc tagcaatatc tgtgattata ttcagggggc tgtacgtatt ggaatggact
      841 ttgttccagg cactcgctct gacttacata gccgttcgct gcagataaaa tatgaggctt
      901 tgaagcatat agaaaaaaac attaatgata atattattta tcatccgagt aataatgcta
      961 atttctattc tgtaattgag tcaattgatg gtaatgactt tatatataac gaaaaacaat
     1021 ctaaaatatt agaaatgaaa caggatcgtg gggggaatag atatagtgca gtagatctta
     1081 actcttctaa gtatgggtat tatgagaaag ttggcggtgg tttttataga tatatagaat
     1141 cctttaaccc catatcttca gagacaccaa ataaaatagt ctacaagggg gaatcagtag
     1201 atttaactaa ggagccaaat tcggaattat attcaggtag gtattctata aataacaaac
     1261 aggttaatgt ttatttcttt cgtgacgctg atggtacatt ttataaatca gaaggtcttc
     1321 atggtggggg agttattaga tacatagata aaccgtattc tcagttaaga gaaggagata
     1381 ttgggtatga tgaggatttg ttggatatat acgatgattc tccggtgctt gaagacacgt
     1441 tgcctgcttt atcttctgaa atagtaccaa ctccagaaca tagtattaaa caaatttatt
     1501 cgaaaattaa ggaggggcac atagaactgt ccgattcaga catcatattg tgtcgcggca
     1561 caaccggtat tcaagctgaa aatatcgttg aatataaaac tgctggaggg cttcctgatt
     1621 caaatccaaa tgtaaaagca ccagatgaat atatggcaca acagcaggta cgtattggaa
     1681 gaatattgcc tgaatacaca tcggatctta gcgttgctga tcggtttagt cgtgagcatt
     1741 atctaatagt tgttaaagta aaggcaaaat atatcacacg aggaagtgtt acagagagtg
     1801 gttgggttat agataagacc gcacctgttg aaccacttgc gataattgat agaacttttg
     1861 gtatgaagga aaatatctca atggtaaatg catcgaaata gtttttttac aatctatgtc
     1921 ctgcctcctc tggtaaaaac gatgctacat cttggagatg ttgcacggca gtacggtgtt
     1981 gaccgatatg tagtgaagta cacatcggtc aacgaattac cattgtcagc aatatcatcc
     2041 tgataaactc agtactcgtg agccgcttga tgacgggcgg aatagcccaa aaggtaaacg
     2101 atccgcatgg cagtccgtcg gaaagctgct gcttcatacg accgcttaaa ccgtcagtta
     2161 gtgtcagtat cgctgaagat cagcttcttt tgctgattta ctctgttttt acctgccctg
     2221 atgaaat
//
LOCUS       DAZFBR010000276         2176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFBR010000276 DAZFBR010000000
VERSION     DAZFBR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.279"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(178..435)
                     /locus_tag="ACHJZ2_005104"
     CDS             complement(178..435)
                     /locus_tag="ACHJZ2_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571008.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(432..929)
                     /locus_tag="ACHJZ2_005105"
     CDS             complement(432..929)
                     /locus_tag="ACHJZ2_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO4571009.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGAHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELHHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPGLIAEINSLANQLQISI
                     PGLPG"
     gene            complement(1132..1569)
                     /locus_tag="ACHJZ2_005106"
     CDS             complement(1132..1569)
                     /locus_tag="ACHJZ2_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4571010.1"
                     /translation="MSRVTVIISALVICIIACLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1566..2063)
                     /gene="rrrD"
                     /locus_tag="ACHJZ2_005107"
     CDS             complement(1566..2063)
                     /gene="rrrD"
                     /locus_tag="ACHJZ2_005107"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO4571011.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(2063..>2176)
                     /locus_tag="ACHJZ2_005108"
     CDS             complement(2063..>2176)
                     /locus_tag="ACHJZ2_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO4571012.1"
                     /translation="QWVAIGVLGSLLFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          510 a          574 c          511 g          581 t
ORIGIN      
        1 cgcagaagga attccccgtc gggctacggt catggttaat gcggaaatac agcgacgata
       61 cagcgcatga tgtgtcaggc ttgaatacct ttatccgtta aaagggatat cagttaagtt
      121 atcccgtgta gggtataagc cattatcaag cccaccagta gatgggcttt gtaatggcta
      181 cttcgctttt gcttccgctc gcttacgccg gcgctcttct ttcctctcgg cttttgccat
      241 gtccatgaat gcctgcatga tcgagttccg catcatgtag ctaacaaagt gatgattgac
      301 acagccgttg aggcgcagct gctcgccaaa ctcatccacc gaggccaatg cttccatcat
      361 acccttctcg cctttcatga actctgagaa gtcgcgcccc gctctggagg cgcattcaat
      421 gacacgatca ctcatcccgg aagcccgggg atcgaaatct gcaactggtt agccagggag
      481 ttaatttcag cgatcaaacc aggtttcgtg tagcgccatg ccgcgaggcc ttgtccacag
      541 aagctcgcca tgtctttttt ctgatcaaac tcatgacatt tcatgttgag ctgcgcactt
      601 aagctgttcc gatgatgaag ctctccggtg aagtaatcat cgaggacttt ataggctgca
      661 tatttaaatc cggggtttag ccatgctgcg taatcataag caacgaactt tccgccataa
      721 gttccaccgt gcgcaccgcg ttccgtaaaa accacagatt cgtggttttt ctccagctca
      781 gccaagaact ctttggtctg cttgtttcgc aggtagtggt acggagattc agattcactt
      841 ttaccactgg ctttccacat atcagtgagg cagatcatgc catcttcacc gatacggatt
      901 ggttgattga agagggttaa tgatttcata gcgtgtacct actctttgaa atgaaccttt
      961 gccgcacagg aaaccagccc accgaggctc gccagcacta actggtatcc tcaaaggccc
     1021 attccaaagg ggcaggttcg gtgtaaaaaa catgcgttgc ggtacgcatt tattgcaaaa
     1081 agccccgcat cgcgaggctc attaaatgga ctttgtgatt tgcaaaaaaa attatttcag
     1141 gcattgcgtc ctgatgtact cctgcaggta gttaacctgc gcggttatct tgtcgattcc
     1201 acctcggaga cggtaataat tgagttcagc atctgctgta agtcctgggc tttctccatc
     1261 gcccatgctg ctggctccgg tcgttgactt tgcacaggtg gcggagactt gcaggcgctt
     1321 acgcccagca gaaacatcag cacggagact ttcgatagtc gcattagcat cagcaagctc
     1381 ctttgtgtat ctggcgtcaa gttctgctac atcacgttgc cgcttctgca tatcagcgat
     1441 gatggatgtg gctttatcgc gctgctcttt gtaggcgatg gcgttatcac ggtaatgatt
     1501 aacagcccat gacaggcagg cgataatgca gataaccaga gcggagataa taacggttac
     1561 cctgctcatt gttgccccca caaacagact tcacgctcaa tctcgcggcg agtcatcagc
     1621 cctttccatt gcttaccgcc agcgtatgtc cagcgccgta gctgatcaca tgcgcctttg
     1681 atatcgccct ggtttatttt gcgaagaagc gtcgatgttc tgaaattgcc agcgcccacg
     1741 ttgtaaacga acgagtaaag agcgccgcgc gttgtttccg gtatatcgac gttgatgtac
     1801 gggttaattt gtctggcgac cgtggcaagg tctttattca ggagggcttt gcattctgct
     1861 tcggtatacg ttttaccggg catgatgtct tttccggtgt gtccgtgaca tacagtccat
     1921 acgccaacga tatctttgta tggtatgtag ctgacacctt ccagaccatc gttaccacct
     1981 ggaccagtga tgagcacaga cgctatggca acagccccac caccaatagc agctgcaaca
     2041 gccttgcgta atgacggcga cattattcac ctctcgcagc cttacgctta tcttctttaa
     2101 tcttgaaata aagatttgtc agatacgtca gcaggccaaa cagcagactt cccagcacac
     2161 ctattgccac ccactg
//
LOCUS       DAZFBR010000277         2165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFBR010000277 DAZFBR010000000
VERSION     DAZFBR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.280"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            14..1540
                     /gene="ybcK"
                     /locus_tag="ACHJZ2_005109"
     CDS             14..1540
                     /gene="ybcK"
                     /locus_tag="ACHJZ2_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4571013.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELKIIELQMKINNLI
                     VALSVAPEVTAIAEKIRLLDKELRRALVSLKTLKSKGVNSFSDFYAIDLTSKNGRELC
                     RTLAYKIFEKIIINTDNKTCDIYFMNGIVFKHYPLMKVISAQQAISALKYMVDGEVYF
                     "
     gene            1947..2045
                     /locus_tag="ACHJZ2_005110"
     CDS             1947..2045
                     /locus_tag="ACHJZ2_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571014.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
     gene            2042..>2165
                     /locus_tag="ACHJZ2_005111"
     CDS             2042..>2165
                     /locus_tag="ACHJZ2_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571015.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRE"
BASE COUNT          712 a          367 c          457 g          629 t
ORIGIN      
        1 agcaggtgtc accatgaaaa aagccatagc atatatgcga ttttcatcac caggtcagat
       61 gtctggcgac tcattaaacc gacagagaag acttattgct gaatggttaa aggtaaatag
      121 tgattattat cttgatacca taacatatga agatttagga ttaagtgcat tcaaaggaaa
      181 gcatgcacaa tcaggagctt tttcggaatt tttagatgct atagagcatg gttatatatt
      241 gccaggaact acattgttag ttgaaagtct ggacagactt tcaagagaaa aagtcggtga
      301 agcgattgaa cgtctgaaat tgattttgaa tcacggtatt gatgttataa ctctttgcga
      361 caatacagtc tataatattg actctttgaa tgagccatat tcattaataa aagccatact
      421 tatagcacaa agggcaaatg aagaaagcga gataaagtca agtcgggtta aattatcatg
      481 gaagaaaaaa cggcaggatg cactggaatc aggtacgatt atgacggcgt cttgtccgag
      541 atggctctcc ttagatgaca aaagaacggc ttttgttcca gaccccgaca gggtgaaaac
      601 tattgagcta atttttaaac tcaggatgga aaggcgctca ttgaatgcaa tagccaagta
      661 tttaaatgat catgctgtaa agaatttctc aggaaaagaa agtgcatggg ggccttctgt
      721 aattgaaaaa ttattagcga ataaagctct gataggtata tgcgtacctt catatcgtgc
      781 aagagggaaa gggataagtg aaatcgctgg ctattatccc agagtcatat cagatgattt
      841 gttttacgct gtacaggaaa ttcggttggc accttttggt attagcaata gtagcaagaa
      901 tcctatgcta ataaatctac ttcgaacagt tatgaagtgt gaggcttgtg gtaataccat
      961 gattgttcat gcggtatctg gaagtttgca tggctattat gtttgtccga tgagaagatt
     1021 acatcgatgt gacaggccat caataaagag agatttggtt gattataata tcattaatga
     1081 attgcttttt aattgtagca aaattcaacc agttgaaaac aagaaagatg ctaatgaaac
     1141 tttagagtta aaaattattg agcttcagat gaaaattaat aatttaatcg ttgcattgtc
     1201 tgtcgcgcct gaagttaccg ctatagcaga gaaaataaga ctattagata aggaattacg
     1261 aagagctttg gtatcattga aaactttgaa gagtaaaggt gtaaattcat tcagtgattt
     1321 ttatgctatt gacttaacca gtaaaaatgg acgagagtta tgccgtacac ttgcctataa
     1381 aatattcgaa aaaatcataa ttaatacgga taataaaacc tgtgatatct attttatgaa
     1441 tggcattgtt tttaaacact atcctttaat gaaagtaata tccgcccagc aggcgataag
     1501 tgctctcaaa tatatggttg atggtgaggt ttatttttga gtaataatca ctttttcaac
     1561 cgtgctatgg tatgaaagta aagtaactac tatgatatta actatcgagc aagaatatcc
     1621 ccaggcactc aacagggcgc aggtcaacgt gaatcggtca tgacacgaca ccgattttgc
     1681 ggaatcaatg ttgcttatga atacaacatc ccgcttagta catgttcaac acatcagcaa
     1741 attttgaact gggtgtggca cttaactgaa aaaacatgga tgacacaaaa tgttactcgt
     1801 cgcttcattg aagtagcttg tggatatcac aagctagact atcgccaatg atattcaatt
     1861 tttgattttt tagtatcaac cttccataat aatgtcaccg gagcttgaac aactccggtg
     1921 acttctgcgc atttaagggg acttaaatgc gaccacaatc tgaactcctt accttgtcac
     1981 agatgcagaa atgcacctgc gatgtcttgc atccagcgtt tgatctctgc ggaggtgaag
     2041 cgtgaacctc ccacaagatg gcatcaaatt acatcgcggt aacttcaccg ctgtcggtca
     2101 gcagatccag acttatctgg aagacggcaa atgctttcgc atggtgctta aaccgtggcg
     2161 cgaga
//
LOCUS       DAZFBR010000278         2131 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFBR010000278 DAZFBR010000000
VERSION     DAZFBR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.281"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..203)
                     /locus_tag="ACHJZ2_005112"
     CDS             complement(<1..203)
                     /locus_tag="ACHJZ2_005112"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571016.1"
                     /translation="MCRKYAHVVDALQGNFPYATDPAGNRLPDPELHPDSTLTAWPDN
                     RIAEDAHYVYRYDEYGRLAEKTDR"
     gene            complement(361..1110)
                     /locus_tag="ACHJZ2_005113"
     CDS             complement(361..1110)
                     /locus_tag="ACHJZ2_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO4571017.1"
                     /translation="MEEANGGEAIVMNCLIFFKPKNYHLKSKLNYYPITQAAFLGETE
                     VVSNLIKLGVDLDARGDLGRTALNEAVSNGYFDIVKLLVESGADLTIKDEFGKTALEL
                     AEVHQNYKIVEWLSHYRDHNPIPDFSVLINIYGERYFGGEIIDTDARTELGEGVLHIA
                     VRKRRQLDVRCFIQHGADINAKANNGFDFTPLHYSIGIGDFDMMKLLLKLGADIYLES
                     EMGYSPMDLAILMGDKRIIFYLYGYISHVSE"
     gene            complement(1217..>2131)
                     /locus_tag="ACHJZ2_005114"
     CDS             complement(1217..>2131)
                     /locus_tag="ACHJZ2_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4571018.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDEESGL
                     YYNRHRYYDPLQGRYITQDPIDIKGGWNLYSYALNPVSWIDPLGLTQCDSEGCNNDIL
                     FTGGSGPDNKILNELGPRDGIDGLGSQNMKMYSGLLGGDILKPGILGGLTLGSVTQRP
                     SRTAEEEVQAQIEYLAYKKRCEQKPDSNLSRCAAAIFQKSRKEDCLRMRQEWDDKWWP
                     GKHADEIKNVKASLKNTLIDVKKKMHAN"
BASE COUNT          562 a          569 c          360 g          640 t
ORIGIN      
        1 cggtccgtct tctccgccag cctgccgtat tcatcgtagc ggtagacata gtgcgcatct
       61 tccgcgatgc ggttatccgg ccatgccgtg agggtgctgt ccgggtgcag ttccgggtcc
      121 ggcagccggt ttcctgccgg gtctgttgca tacgggaaat tcccttgcag ggcgtccaca
      181 acatgtgcat atttcctgca catgctgtgg ttttgtcata caaggagcaa caatactagt
      241 taatgattca taaaaaattt taacggggtg atttttcatg cgattatcgt cctaattaac
      301 gccacctcac ttacagatta tttcattcgt tacaatcttt tgattgcatt acacccaggc
      361 ttattcagaa acatgagaaa tataaccata taaataaaaa ataattctct tatcccccat
      421 caaaatagcc aaatccatag gggagtatcc catctcactc tccaaataaa tatcagcacc
      481 taatttcaac aaaagcttca tcatatcaaa atcccctatc cctatcgaat aatgcaaagg
      541 agtgaagtca aagccattat ttgccttagc atttatatcg gcaccatgtt gaataaaaca
      601 tctgacatct aattgtcttc ttttcctaac agctatatga agaaccccct cgccaagttc
      661 tgtccttgca tctgtatcta taatctctcc accaaaatat cgctcaccgt agatattaat
      721 aagtacagaa aaatcgggta tgggattatg atctctataa tgtgatagcc attcaactat
      781 tttataattc tggtgtacct cagccaattc taatgccgtt ttcccaaatt catcctttat
      841 agtaagatcg gctcctgatt ctactaaaag cttaacaata tcaaaatacc cattacttac
      901 ggcctcatta agtgcggtac gccccaaatc cccacgagca tccagatcca cacccaattt
      961 aattaaatta cttacaacct cagtctcacc taaaaatgca gcctgagtta taggataata
     1021 atttaatttt gacttaaggt ggtaattttt cggcttaaaa aaaatcaggc aattcatcac
     1081 tatagcctcc cccccattgg cttcctccat aaatcggcag catattaaag ataagaaaat
     1141 atattatagc attttcacca atcaatgggc ataattaaca tcattatatt ttaaggatac
     1201 ctatctcgga atgccactag ttggcatgca tctttttttt aacatcaata agtgtgtttt
     1261 tcaacgatgc tttcacattt tttatttcat ccgcatgttt gccaggccac catttatcat
     1321 cccattcttg tctcattctt aaacaatctt cttttctgct cttttgaaag atcgcggcag
     1381 cacaacgact taaattacta tcaggttttt gctcacaacg cttcttatat gctaaatatt
     1441 ctatttgagc ttgcacctcc tcctcagccg ttcgcgatgg tcgttgtgtc acactaccaa
     1501 gcgtcaaacc gcccagaatc cccggtttta aaatatcgcc ccctaatagc ccactataca
     1561 ttttcatgtt ttgtgaaccc aaaccgtcaa tgccatctct cggacctaat tcattaagta
     1621 ttttattatc gggaccactt cctcctgtaa ataatatatc attattacac ccttcggaat
     1681 cgcactgcgt caatcctaat gggtcaatcc aacttaccgg attaagcgca taagaatata
     1741 aattccatcc tccttttata tctattggat cctgggtgat atacctcccc tgtagcgggt
     1801 catagtaccg gtgacggttg taatacagcc ccgactcctc atcatactgc tgtcccggca
     1861 gacggtacgg ctgttgcagg tgctgcgcgc tggtttcgcc cagcaggttt ccccactcgt
     1921 catactcccc ctgccacgcc gtttcccctt ccgggctgat gagcgcctgc ggcagtcccc
     1981 ggtggtcgca gtggtaaaga tgaagtttcc gctccgggat atattccgcc tccagctgcg
     2041 cggccatctg ttccgccgtc agcccgcact gcgcaagcca ctgctggctt tcttcactca
     2101 cgctgccctg ccgcagttcc cgctccagcc t
//
LOCUS       DAZFBR010000279         2112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFBR010000279 DAZFBR010000000
VERSION     DAZFBR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.282"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..267)
                     /locus_tag="ACHJZ2_005115"
     CDS             complement(<1..267)
                     /locus_tag="ACHJZ2_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HFO4571019.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKSEYFIITKSSPVRA
                     ILNDFAANYSIPVFISSSVNDDFSGEIKNEKPVKVLEKLSKLYHL"
     gene            complement(267..722)
                     /gene="sepD"
                     /locus_tag="ACHJZ2_005116"
     CDS             complement(267..722)
                     /gene="sepD"
                     /locus_tag="ACHJZ2_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO4571020.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSNEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQLSSSDTMMPPTHLFSDIGRIV"
     gene            complement(728..1300)
                     /gene="escJ"
                     /locus_tag="ACHJZ2_005117"
     CDS             complement(728..1300)
                     /gene="escJ"
                     /locus_tag="ACHJZ2_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO4571021.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(1303..1680)
                     /gene="sctI"
                     /locus_tag="ACHJZ2_005118"
     CDS             complement(1303..1680)
                     /gene="sctI"
                     /locus_tag="ACHJZ2_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO4571022.1"
                     /translation="MNNINQSENINIQLNKAPQTNFVDEHTSLASAPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNSDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            complement(1764..2063)
                     /gene="espZ"
                     /locus_tag="ACHJZ2_005119"
                     /gene_synonym="sepZ"
     CDS             complement(1764..2063)
                     /gene="espZ"
                     /locus_tag="ACHJZ2_005119"
                     /gene_synonym="sepZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE cytoprotective
                     effector EspZ"
                     /protein_id="HFO4571023.1"
                     /translation="MEAANLSPSGAVMPLATSLSGNNSVDEKTGVIKPENGTNRTVRV
                     IAGLALTTTALAALGTGIAAACSETSSTEYLALGITSGVLGTLTAVGGALAMKYA"
BASE COUNT          586 a          406 c          413 g          707 t
ORIGIN      
        1 gagatgatat agtttggaca gtttttccag tacttttaca ggtttttcat tttttatttc
       61 gccagagaaa tcatcattca cggaactact gatgaaaaca ggaatgctgt agttagcagc
      121 aaagtcatta aggattgcac gtactggaga acttttagta ataataaaat attcgctctt
      181 gcctaatctt ttttccagag aggacggggc agcttgtgca ctgcagcaaa atagtgctgt
      241 aaaaataaaa aaacttattt ttttcattac acaattcgtc ctatatcaga aaacaagtgt
      301 gttggcggca tcattgtatc cgatgaagat aattgctcta gtttatcaaa ttgcttatcg
      361 agcaaagagg cgagagtatg ttgcattatt gcttttgaca tgtttgcgcg atgattatta
      421 tctttatgaa aaatttggca aagataccac ttcttattag acaggaaaat acccatttgt
      481 ttatattcgt tactaacggc caggatattg cagagtctga taaatgtatc tgttggaacc
      541 tgacgaaaat caccactaag ctcaatatat agaacccagt agtttccaaa atcgtgcaag
      601 aaagcatttt tttggtagat ataaaaagtg agatgcgttg gtgaaccgtt gacatccttt
      661 acaaaatcaa gggccgttag ccagtcacaa tcattctttg ctatgccatt attattgttc
      721 atacatatta cccgtcctgt cctgaggatg acttgataac aacactaata ttttctaatt
      781 tgagatcgtc aacactattc ttaactaggt tttttatctg aatgaccgat ggtgctaagt
      841 taacctctgg cgagctgatg actaaaacgg ctgctgagga gggttgactt tcgttattgt
      901 taacatttaa tgaaacgctg caatcgataa caccaggtat tttacttagc aacctttcga
      961 tgtcttgttc tttgagatag ttgatttttg cattttcttg tgatggcgat gccactaact
     1021 ggctaggtgg gaaaataacc tcgatatctg caaatttctt tttcgggaag ccgttattat
     1081 ttaaaatcgt tattgcgcga acaaaatcct ctttttccac actgagtgtc atatttccac
     1141 ttttgtccat ttctttggaa acattgacat cattggatag caacagtgct tgcatctgat
     1201 tagcttcttt ttctgttaat cctgtataca attgctcttt acatgccact gtcaaacaaa
     1261 tagcagccaa taaaaaaagg tttttaatgt gttttttcat ttttattgca tcgaaactaa
     1321 ttttgtaacc gatgttgaaa ggaggcttgc taacttagat acgacatcaa cgttgacgtt
     1381 tgcgttgagc acattctttt gtaaggtgag cacgtcttct ggcgagagcc cacctgcaga
     1441 tagagcatcg aaactgagat cgctgttcat tgttgcaaga tgtctctttt taatttcttc
     1501 aatcaacact tgttctggag aagacactcc cgctgtttta gggagtaatt gatcaagaaa
     1561 ctgagccgca cctgccgcag agggggctga ggctaacgag gtatgctcat caacgaaatt
     1621 tgtttggggc gctttattta attgaatatt aatgttttcc gattgattaa tgttgttcat
     1681 atttactgtt agcctctttt tatgaaagag gcaagggcat aatacatcca ttacctctat
     1741 cttttattta tgcgatgggc cggttaggca tatttcatcg ctaatgcacc gccaaccgca
     1801 gtaagagtac ctagtacgcc agaagtaata cccagggcta agtattctgt gctgctcgtc
     1861 tccgagcatg ccgctgcaat acctgtacct agagctgcca gagccgtagt ggtaagtgct
     1921 aatccggcta taactctaac ggtgcgattt gttccatttt ctggtttaat cactcctgtc
     1981 ttctcatcca ctgagttatt tccactgagt gaggtcgcca gcggcattac tgcaccagaa
     2041 ggacttaaat ttgctgcttc cattgatctt tctccttttg tctaatttag acatttacct
     2101 ggttataaat aa
//
LOCUS       DAZFBR010000280         2111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFBR010000280 DAZFBR010000000
VERSION     DAZFBR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.283"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            321..866
                     /locus_tag="ACHJZ2_005120"
     CDS             321..866
                     /locus_tag="ACHJZ2_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO4571024.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            841..>2111
                     /locus_tag="ACHJZ2_005121"
     CDS             841..>2111
                     /locus_tag="ACHJZ2_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4571025.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLI"
BASE COUNT          507 a          500 c          607 g          497 t
ORIGIN      
        1 gatcaattaa tacgatacct gcgtcataat taattatttg acgtggtttg atggcgtaga
       61 tgcacgttgt gacatgtaga tgataattat tatcattttg cgggtccttt ccggcgatcc
      121 gacaggttac ggggcggcga cctcgcggtt tttcactatt tatgaaaatt ttccggttta
      181 aggcgtttcc gttcttcttc gccgtaacct aatgttttta tttaaaacac cccctgaaaa
      241 gaaaggaaac gacaggtgct gaaaacgggc tttttggcct ctgtcgtttc ctttctctgt
      301 ttttgtccgt ggaatgaaca atggaagtca acaaaaagca gctggctgac attttcggcg
      361 cgagtatccg taccattcag aactggcagg agcagggaat gcccgttctg cggggtggtg
      421 gcaagggtaa tgaggtactt tatgactctg ccgccgccat aaaatggtat gccgaaaggg
      481 atgctgaaat tgagaacgaa aagctgcgcc gggaagttga agaactgagg caggacagcg
      541 agacagatct ccagccaggg actattgagt acgaacgcca tcgacttacg cgtgcgcagg
      601 ccgacgcaca ggaactgaag aatgccagag actcagctga agtggtggaa accgcattct
      661 gtactttcgt gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggatccccc
      721 tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg
      781 atatcatcaa agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga
      841 gtgaatatat cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc
      901 tcactgttca ggccggagcc acagaccgcc gttgaatggg cggatgccag ttactatctc
      961 ccgaaagaat ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc
     1021 atgaatgcga tgggcagcga ctacatccgc gaggtgaatg tggtgaagtc tgcccgtgtt
     1081 ggttattcca aaatgctgct gggtgtttat gcctacttca tagagcataa gcagcgcaac
     1141 acccttatct ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag
     1201 ccgactattc gtgatattcc gtcgctgctg gcgctggccc cgtggtatgg caaaaagcac
     1261 cgggataaca cgctcaccat gaagcgtttc accaatgggc gtggcttctg gtgcctgggc
     1321 ggtaaagcgg caaaaaacta ccgtgaaaag tcagtggatg tggcgggtta tgatgaactt
     1381 gctgcctttg atgaggatat tgaacaggaa ggctctccga cgttcctggg cgataagcgt
     1441 attgaaggct cggtctggcc aaagtccatc cgtggctcca cgcccaaagt gagaggcacc
     1501 tgccagattg agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc
     1561 ccgcactgcg gggaggagca gtaccttaaa tttggcgata aagagacgcc gtttggcctc
     1621 aaatggacgc cggatgatcc ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc
     1681 atccgtcagc aggagctgga ctttactgat gcccgttata tctgcgaaaa gaccgggatc
     1741 tggacccgtg atggcattct ctggttttcg tcatccggtg aagagattga accgcctgac
     1801 agtgtgacct ttcacatctg gacggcgtac agcccgttca ccacctgggt tcagattgtc
     1861 aaagactgga tgaagacgaa aggggatacg ggaaaacgta aaaccttcgt gaacaccacg
     1921 ctcggtgaga catgggaagc gaagatcggc gaacgtccgg atgctgaagt gatggcagag
     1981 cggaaagagc attattcagc gtccgttcct gaccgtgtgg cttacctgac cgccggtatc
     2041 gactcccagc tggatcgcta cgaaatgcgc gtatggggat gggggccggg tgaggaaagc
     2101 tggctgattg a
//
LOCUS       DAZFBR010000281         2088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFBR010000281 DAZFBR010000000
VERSION     DAZFBR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.284"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1481
                     /locus_tag="ACHJZ2_005122"
     CDS             <1..1481
                     /locus_tag="ACHJZ2_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4571026.1"
                     /translation="YYSQCLQTLIESIELLLDEALETGENESTEFDVTTLLRMDSERR
                     MKTLGDAVKNTLLTPNEARKRENLPPLAGGDALYLQQQNYSLEALSRRDAREDPFASA
                     GKTVSSQLPDGASDGNKAISETEHDAVKAMFRGDTEKMTERELSIIRALGEEFSTVLA
                     DLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVTVDEVRPVLQAMVDDAVGAIPVPRDG
                     RDYDPDVLQQAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIPVPRDGRDYD
                     PEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIPVPRDGRDYDPDVL
                     QKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRGTYATHQGGLWRAYEKTHGMR
                     GWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPG
                     DTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDARGGK"
     gene            1478..1804
                     /locus_tag="ACHJZ2_005123"
     CDS             1478..1804
                     /locus_tag="ACHJZ2_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4571027.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            1814..>2088
                     /locus_tag="ACHJZ2_005124"
     CDS             1814..>2088
                     /locus_tag="ACHJZ2_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFO4571028.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRIL"
BASE COUNT          477 a          488 c          700 g          423 t
ORIGIN      
        1 agtattattc ccagtgcctg cagacgctga ttgagtccat tgaactgtta ctggatgagg
       61 cgctggaaac gggggaaaac gagagtacag aatttgatgt caccacgctg ctgagaatgg
      121 acagtgagcg gcgcatgaaa acgctggggg atgcggtgaa aaatacgctt ctcacgccca
      181 atgaggcccg taaacgggag aacctgccgc ccctggccgg cggtgatgca ctgtatcttc
      241 agcagcagaa ctacagtctg gaggcgctgt cccgtcgtga tgcccgtgag gatccgttcg
      301 cgtctgccgg gaaaacagtt tcatcacagc tgcctgacgg cgcatctgac ggtaataagg
      361 caatcagtga aacagagcat gatgcggtga aagcgatgtt caggggggat actgagaaaa
      421 tgacggaacg ggaactgtcc attattcgtg cactgggaga agaattttcc acagtgctgg
      481 cggatttaca gcgcacattt gaggggaaga tggcctcgca ggcacaagcg tttgaagaga
      541 aactgacttc cctgtcggcg gtattacaga agcatgtgac ggtggatgag gtgcgtccgg
      601 ttctgcaggc gatggtggat gacgctgtgg gggccattcc ggtaccgcgt gatggtcgtg
      661 attatgatcc ggatgtactg cagcaggcgg tgaatgatgc ggtcgcaaat attccgcagc
      721 cggcggacgg taaaagtctc accccggatg atgtgcgtcc gatgcttgaa cagatggtga
      781 aggaggcggt aagccatatc cctgttccgc gcgacggtcg tgactatgat ccggaagtac
      841 tgcagaaggc ggtgaatgat gcggtcgcaa atattccgca gccggcagac ggtaaaagtc
      901 tcaccccgga tgatgtgcgt ccgatgcttg aacagatggt gaaggaggcg gtaagccata
      961 ttcctgttcc gcgtgatggt cgtgactacg atccggatgt tctgcagaag gcggttctgg
     1021 atgcggtgag tgccctgccg gctccgcagg acgggcgtga tgccacggca ctggaaatac
     1081 tccccgccat tgacgatcaa aaatcctttc cccggggcac gtatgccaca caccagggcg
     1141 gactctggcg ggcgtatgaa aaaacgcacg ggatgcgggg atgggaatgc ctggttgacg
     1201 gggtggcgga tattgacgtc agcatgacgg gtgagcggtt gttctctgtg gtggtccggc
     1261 agagcagtgg ccagcgtacg gaaaaaacat tttccctgcc ggtgatgctc taccgcggtg
     1321 tgttcagagc cggtgaaacc taccaccccg gcgatacggt gacgtggggg ggctcgctgt
     1381 ggcactgcaa cagtatgacc gaagataaac ccggagaagc tcattcatca gcctggaccc
     1441 tggctgcaaa acgtgggcgg gatgcaagag gtggaaaatg acggcattac tgacactgga
     1501 agagatcaag gcacatctgc gtgtcgacca tgacgcggat gatgacatgc tgatggacaa
     1561 ggttcgtcag gctaccgccg tgctgctggc ctacattcag ggcagccggg ataaggtgat
     1621 tcgtgaggac ggtgaactga tcccgggcga ggcattaacc cggatgaagg gggctgccat
     1681 gcgactgacc gggatgctgt accggaatcc ggatcttgcg gagcgggaag aactgattca
     1741 gggggaactg ccgttttctg tttccgtgct gatttacgat ttgcgttgtc cgacggtgtt
     1801 atgaggaggg ggaatggcaa tatctgcagg tcgtctgaca cagatgataa gtgttctgaa
     1861 cccggtgtta acccgtaacg ctgccggaga aatgacggaa gaatgggtgt catgcgggaa
     1921 aattcatgcg gatatccggg gcaggagcag ccgggagcgg atgcagtccg gtgcggaaat
     1981 ggcgcaggcg gaaatccgca tctgggtgcg cggtcagtcc ggtcgggaaa tcacggcagc
     2041 gtcacgactt catgtgctga gtggtccctg gcgtgaccgg atcctgaa
//
LOCUS       DAZFBR010000282         2073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFBR010000282 DAZFBR010000000
VERSION     DAZFBR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.285"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            158..1300
                     /locus_tag="ACHJZ2_005125"
     CDS             158..1300
                     /locus_tag="ACHJZ2_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFO4571029.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            1774..1874
                     /gene="ykgR"
                     /locus_tag="ACHJZ2_005126"
                     /pseudo
     CDS             1774..1874
                     /gene="ykgR"
                     /locus_tag="ACHJZ2_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
BASE COUNT          660 a          440 c          415 g          558 t
ORIGIN      
        1 aagaaatttc attaaaaaac aaaaaagcag caactcaatt attagaaacc ctttaaaggg
       61 attataaata aagccagaac tcttggttcc aaagctggca acaactaacc agttaaaaca
      121 tacgcatcaa aatcagataa acatttgagg tattgttatg acaaataaac acccatcatt
      181 gttttctcct tttatgctga cagaaaaaat aaagctacgt aatcgtatcg ttatggctcc
      241 tatgacgacc tggtcagcga atcctgatgg aaccatctct gagcaagaac tcgaatttta
      301 taaacgtcgc tcacaaaatg ttggtttagt aataacagga tgtacctatg tcacgccaag
      361 cggcattggg tttactcatg aatttgccgc gtatgacgac cgtttcatta acagccttga
      421 aaagttagca gcggcagcca aaagtggcgg tgctccggct attttgcaga ttttccacgc
      481 cggtaataaa gccatcccgg agcttgtacc aaataacgat gttattagtg ccagcgcatc
      541 aagcgtaaaa tctggtgact ttatgaagag agtggtacaa agcagggaaa tgactgaaaa
      601 cgaaattcag gaaaccatta gagcattcgg agatgtcaca aaaagggcga ttaaagcagg
      661 ttttgatggc attgaacttc atggtgcgca cggattcctt ctacagaatt ttttctcccc
      721 tctatttaac cagcgtaatg accgatgggg aggagatctc gaaggacgta tgcgcttccc
      781 gctggccgta ttacaggaag taaaaaatgt agtctatgaa tatgcgacaa agccctttgc
      841 catcggttac agaatatctc ctgaagaatc tgcaacaggg gggctgcgga tagaggatac
      901 ttataaactc cttgatcggc tcatttcatc cggtatttcg tatatacata cctcgctggt
      961 cagtattaac gatagctacc ctgttgagtc gccaaatggc ccccgaacta ttgaattgat
     1021 tttaaatcat attgcaggca gagtgccggt aatcgctgca ggtaaaatac gcacaccgtc
     1081 tcaggctcaa gaagcaattt ccactggcct gccacttgtt gcgatcggta aagggctggt
     1141 gataaatcca gaatgggtga ctttagctga atcaggtaga ggccatgaaa ttcaaacaac
     1201 ccttaatcca caacttgttc ctgaattaac cattccggat aaactatggg atcaaattca
     1261 ggcatcgaaa ggaactggct ggtttccgtt aatggattaa atatctatta ctctcccgag
     1321 agtaaaacta tcaaatcaat gagtaaccga tacagcctca caaattcttt cagataatta
     1381 acccgtcact gcggcacgtt ctctcataag gcgtgcctgc agtgtaatct agtatgtatc
     1441 cggcatagtt gacggtttaa tagagctcaa ttcctcgccc aggttaccaa caacgatatc
     1501 ttgtataaca ccccaggaga tacgacatta tgatgagatg cgatatgctc ctcagcgcca
     1561 atcaacgcca ggactgggcc agtcttgaga tcagactcat tcccggaact gacttcaggc
     1621 gacatatatc aatgacgttg gggcaataaa agatgcctga tgtaatacat ctattcaaat
     1681 ttgtgataca tgtcaaaaaa ttggttgacc aaactcgtta tttatataag ggcacttacg
     1741 aagtgcgctc ttttttaaag cgaggaagta ccaatgaaag agaataaagt acaacaaatc
     1801 agtcataaac tgattaatat cgttgttttg tcgcaattgt agaatacgcc tatttatttc
     1861 tccatttcta ttaataacgg caataaactg ttcacttcag tgatatttaa aatatgcatc
     1921 ctctcccttt ttgtaagtaa ttattatatc cgtgggagag gaatacacgc tgtcaggtaa
     1981 tcaatcatac tgcgataaat catcggccag taaagtggag ataacctcca ttctcgaaaa
     2041 atccatactc tcagcgaaac tatcatcaat cac
//
LOCUS       DAZFBR010000283         2072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFBR010000283 DAZFBR010000000
VERSION     DAZFBR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.286"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..78
                     /locus_tag="ACHJZ2_005127"
     CDS             <1..78
                     /locus_tag="ACHJZ2_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4571030.1"
                     /translation="VCREWSVTDNARYSDFSCTIEQVVN"
     gene            78..776
                     /locus_tag="ACHJZ2_005128"
     CDS             78..776
                     /locus_tag="ACHJZ2_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4571031.1"
                     /translation="MQNIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KREPVTWQGRQYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTPDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(793..1113)
                     /locus_tag="ACHJZ2_005129"
     CDS             complement(793..1113)
                     /locus_tag="ACHJZ2_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4571032.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            1221..1394
                     /locus_tag="ACHJZ2_005130"
     CDS             1221..1394
                     /locus_tag="ACHJZ2_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4571033.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            1465..>2072
                     /locus_tag="ACHJZ2_005131"
     CDS             1465..>2072
                     /locus_tag="ACHJZ2_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO4571034.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGEL"
BASE COUNT          514 a          458 c          562 g          538 t
ORIGIN      
        1 gtgtgccgtg agtggagtgt cacggataat gcccggtaca gtgatttcag ttgcacgata
       61 gagcaggtgg tgaactgatg cagaatattc atgaagaaag cctgaacgag tcggttaagt
      121 cagagcagtc accgcgggtg gtgctctggg aaatcgacct gacggtgcag ggcggtgagc
      181 ggtatttttt ctgcaatgag ctgaatgaaa aaagggagcc ggtgacctgg caggggcggc
      241 aatatcaggc gtacccgatt gagggcagtg gctttgagat gaacggaaag ggcagcagtg
      301 cccgcccgtc gctgacggtg tccaatctgt tcggtcttgt caccgggatg gcggaggatt
      361 tgcagagcct ggtgggtgcc acggtggtcc gccgccgggt gtatgcccgt tttctggatg
      421 cggtgaactt tgtggcaggc aatccggagg ccgacccgga gcaggagctg actgaccgct
      481 gggtggtgga gcagatgtca tcgctgacgg ccatgacggc ctcgtttgtg ctggccacac
      541 cgaccgagac ggacggggcg ctgtttcccg gtcgcatcat gctggcgaac acctgtatgt
      601 gggattaccg gggagatgaa tgcgggtata acggtcctgc ggtggcggat gagttcgaca
      661 accccacccc ggatatccgt aaggacagat gcagcaagtg catgcgcggg tgtgaactgc
      721 gcaggaatgt cggcaatttt ggcggtttcc tttccattaa taaactttcg cagtaatgga
      781 ttatgcccac cgtcaggtgg gttttttatt tagtagttct ctcaactttt cgttctgctc
      841 tctgaacttt tcctgtattt ctttttgcat ggcgataacc tgggcttgaa gttgaactag
      901 cgcatcaaca tttagcggaa ccgagacgct gttaatttta tctgctattt ccccgagtgt
      961 attttctgca ttcaaggcat cttccagtat ctgaacaatc tctgagttca tagagcgccc
     1021 gtttcgtttg gctcgttcag ctatagcatc tcgcattccg tcaggaaaac ggaggttgaa
     1081 cttgtcgtag tcttttactt gtttttcggc cattgcaaat ctctcaaaaa aaatcatggt
     1141 gccatattgc catacgattt caatggtggc attatggcct tcatggcgtc actttggcac
     1201 caaataaagg agattagata atgcaagata cacttttcac tgagcgcaaa aatatcaaac
     1261 tcaaccttcg ccttccatca cggctgaatg aagaccttcg ccgcctggcg gaaatggact
     1321 gtatatctct gaactctgca attgttcgtt tgctggcaaa aggtgttagg gaagaggtgg
     1381 cgaatggtcg ctaaaaacag cgaagcctca atggctgcaa ccattgaggc ttctaaatta
     1441 ccagttaacc acgagaaaac tgatatgact agtttagcaa ttgcagatcg cacaatcaat
     1501 gttccattcc acggaacaaa tctctttttg gttggaatta acaatgagcc ttatgttcct
     1561 atgaagcctg ttgttgaagg tatggggatg gtttgggctg ctcaatttgt taagttaaaa
     1621 cagaggtttg tcaaaggtat ttcggaaatc gaaatacctt ctgctggcgg taaacagtta
     1681 atgacatgtc ttgcctttcg taaatttgcg gcttggcttt caagcattca accaaacaaa
     1741 gtccgccctg aaatccgcga caaggtaatc cagtatcagg aagagtgtga cgatgtgctc
     1801 tacgagtact ggactaaagg ccatgtgatt aacccgcgca aagctaaaaa ggcattgccg
     1861 gggaaaatca ccactgaaca gcaggaagcc attaaacaac tcgtcatgag tcgcggtcag
     1921 tctctgccaa aagaaaaaca ggcgaaggcg atgatcacca tgtggtcgtc actgaaatct
     1981 cattttgggt gttcatacaa agaaatcagc gaggagcagt ttaccgaagc tctgtcactt
     2041 gctgctcgcg ttccgcttga aggagagtta at
//
LOCUS       DAZFBR010000284         2050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFBR010000284 DAZFBR010000000
VERSION     DAZFBR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.287"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            181..1875
                     /gene="tir"
                     /locus_tag="ACHJZ2_005132"
     CDS             181..1875
                     /gene="tir"
                     /locus_tag="ACHJZ2_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO4571035.1"
                     /translation="MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS
                     RALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNEGFEVLHDHGPLDTLNRQ
                     IGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGG
                     AGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGESKGVGELRESNSGAEN
                     TTETQTSTSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDA
                     AASATETATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEE
                     AKQQAIENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRK
                     NQPVEQTTTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSST
                     FFDTSSIGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTD
                     NGARLLGNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV"
BASE COUNT          586 a          421 c          521 g          522 t
ORIGIN      
        1 tttgttaccc gatgtgtgtt tttagcacat tattttcttg catcaaaaat cagattgtga
       61 tttatttggt cgatgtcatg ttcagccgtt tatcgactac gtgctgtata ccatacatta
      121 ttatgtaata gcaaaataaa ggattgttat ctatttacat aaaataaaaa ggagatattt
      181 atgcctattg gtaatcttgg tcataatccc aatgtgaata attcaattcc tcctgcacct
      241 ccattacctt cacaaaccga cggtgcaggg gggcgtggtc agctcattaa ctctacgggg
      301 ccgttgggat ctcgtgcgct atttacgcct gtaaggaatt ctatggctga ttctggcgac
      361 aatcgtgcca gtgatgttcc tggacttcct gtaaatccga tgcgcctggc ggcgtctgag
      421 ataacactga atgaaggatt tgaagttctt catgatcatg gtccgctcga tactcttaac
      481 aggcagattg gctcttcggt atttcgagtt gaaactcagg aagatggtaa acatattgct
      541 gtcggtcaga ggaatggtgt tgagacctct gttgttttaa gtgatcaaga gtacgctcgc
      601 ttgcagtcca ttgatcctga aggtaaagac aaatttgtat ttactggagg ccgtggtggt
      661 gctgggcatg ctatggtcac cgttgcttca gatatcacgg aagcccgcca aaggatactg
      721 gagctgttag agcccaaagg gaccggggag tccaaaggtg ctggggagtc aaaaggcgtt
      781 ggggagtcaa aaggcgttgg ggagttgagg gagtcaaata gcggtgcgga aaacaccaca
      841 gaaactcaga cctcaacctc aacttccagc cttcgttcag atcctaaact ttggttggcg
      901 ttggggactg ttgctacagg tctgataggg ttggcggcga cgggtattgt acaggcgctt
      961 gcattgacgc cggagccgga tagcccaacc acgaccgacc ctgatgcagc tgcaagtgca
     1021 actgaaactg cgacaagaga tcagttaacg aaagaagcgt tccagaaccc agataatcaa
     1081 aaagttaata tcgatgagct cggaaatgcg attccgtcag gggtattgaa agatgatgtt
     1141 gttgcgaata tagaagagca ggctaaagca gcaggcgaag aggccaaaca gcaagccatt
     1201 gaaaataatg ctcaggcgca aaaaaaatat gatgaacaac aagctaaacg ccaggaggag
     1261 ctgaaagttt catcgggggc tggctacggt cttagtggcg cattgattct tggtggggga
     1321 attggtgttg ccgtcaccgc tgcgcttcat cgaaaaaatc agccggtaga acaaacaaca
     1381 acaacaacta ctacaactac aactacaagc gcacgtacgg tagagaataa gcctgcaaat
     1441 aatacacctg cacagggcaa tgtagatacc cctgggtcag aagataccat ggagagcaga
     1501 cgtagctcga tggctagcac ctcgtcgact ttctttgaca cttccagcat agggaccgtg
     1561 cagaatccgt atgctgatgt taaaacatcg ctgcatgatt cgcaggtgcc gacttctaat
     1621 tctaatacgt ctgttcagaa tatggggaat acagattctg ttgtatatag caccattcaa
     1681 catcctcccc gggatactac tgataacggc gcacggttat taggaaatcc aagtgcgggg
     1741 attcaaagca cttatgcgcg tctggcgcta agtggtggat tacgccatga catgggagga
     1801 ttaacggggg ggagtaatag cgctgtgaat acttcgaata acccaccagc gccgggatcc
     1861 catcgtttcg tctaaatata tccataatca ttttatttag agggagggag gggggaagtc
     1921 taactaacgt caatttttca gggaataaca ttgctgcagg atgggcaact cttgagcttc
     1981 tgtaaatata aatttaatta agagaaaata caatgtcatc aagatctgaa cttttattag
     2041 aaaaatttgc
//
LOCUS       DAZFBR010000285         2049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFBR010000285 DAZFBR010000000
VERSION     DAZFBR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.288"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            1..125
                     /locus_tag="ACHJZ2_005133"
                     /pseudo
     CDS             1..125
                     /locus_tag="ACHJZ2_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(320..487)
                     /locus_tag="ACHJZ2_005134"
     CDS             complement(320..487)
                     /locus_tag="ACHJZ2_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO4571036.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(484..915)
                     /locus_tag="ACHJZ2_005135"
     CDS             complement(484..915)
                     /locus_tag="ACHJZ2_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFO4571037.1"
                     /translation="MFGIFKKKTRRAATEIKKFEKRDLAQVVINAAYLVACADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(1085..1161)
                     /locus_tag="ACHJZ2_005136"
     tRNA            complement(1085..1161)
                     /locus_tag="ACHJZ2_005136"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1125..1127),aa:Arg,seq:tct)
     gene            complement(1262..1337)
                     /locus_tag="ACHJZ2_005137"
     tRNA            complement(1262..1337)
                     /locus_tag="ACHJZ2_005137"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1302..1304),aa:Ile,seq:cat)
     gene            complement(1366..>2049)
                     /locus_tag="ACHJZ2_005138"
     CDS             complement(1366..>2049)
                     /locus_tag="ACHJZ2_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4571038.1"
                     /translation="CSPINIFLEKDTLSLKPGSVVLATKCIRELFLMHYGKVKIVDIS
                     ESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGEVTDMFLMMAC
                     LSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHMSESLLKTKLK
                     NEGGMFSRLLLEERMRVAVNMLCSRYGYGQAVAEKCGYSSRSYFISVFHRYYGFPPDR
                     YVSRQGLDY"
BASE COUNT          560 a          558 c          452 g          479 t
ORIGIN      
        1 cgacatcgaa tttctcccgt gtacaggaac agagttaaaa agccggaacc ggaatcaaat
       61 cacaggatga ccatctgcca gtggctggtc gtaaaaaaaa gccgcgccat gcgcagccgg
      121 aaataaaggg ataacgatga tagtttgaga aaaacagaaa caacactttt gtggcaaagc
      181 atggtgccgg gtgcctcccg gtgaattcag tatcagcacc tgaatccgcg attatcccat
      241 atacctggtt gctgatcgcc cctccgcaca gggggattca ccatgcagaa gtgtttttaa
      301 taaacagcaa acaaaaaaat caagcattat gcaggctgtt tctttttatc accggccaca
      361 gcaataccac aatgccgcag accagcaccc catccgccag caccgacatg attctgctgg
      421 tgaaatccac catcaccacc agaaacagca ggagtgcagc cacagccagg cgcagtttta
      481 ccgtcacagg tgattctcca gacgaagacc cagaacaccg gcaatctctt ccagcacctt
      541 gcgctcttcc ggctcaattt cgccgtctgc ctccgcaatg gccaccgcca catccagcac
      601 atcttccgct tcacgcgtat cgtgtttcac atcctcaatc tcacgtaacg ccgcacgacg
      661 accagtttta aagttcgtat ccagctgacc gataatggtt gcgctaatcg cattaatttc
      721 tgacgtaaac gcggacagcg caggctgatt acgcagtacc tgttcgatct tcgctttctc
      781 ggatgcctca cattcaccat ctgcacaggc caccaggtat gcggcgttaa tcaccacctg
      841 tgccagatcg cgtttttcaa actttttaat ttccgttgcc gctctgcggg ttttcttttt
      901 gaagattcca aacatcgtga cgttcctttg ggtgggtgag ccaacgcccg ggagcgatct
      961 gcccacagag aaagtcacac tgaccactcc gtaagctcac ccccgaaagg ctctgtggtt
     1021 ggtatgcgcc gggcgtggcg cagatacaaa aaaggcccgc cgaagcgagc ctggaaaata
     1081 agtgtggcgc gttgtagtgg agtcgaacca ctgaccgatt gcttagaagg caattgctct
     1141 gtccggctga gctaacaacg cagaataccg ataatggacc gccatcggga acccggcccc
     1201 gcaccaacaa ccctgttatg gtgtcgtctg ctcttcctga taagctaatg gcggtttgtg
     1261 atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc tcgctctcac
     1321 cactgagcta aagggccgag ccaaaaaata ataatcagat gaaaatcaat aatcaagccc
     1381 ttgcctggat acatatctgt ctggcgggaa gccataatag cggtgaaata cagaaataaa
     1441 gtaggaccgg cttgaataac cacatttttc tgctacagcc tgtccatatc cataccggga
     1501 acataacata tttacagcaa cacgcatccg ctcttccagc aacaagcgac tgaacatgcc
     1561 cccttcattt ttcagttttg tctttaacaa actctcactc atatgcaact gtagagcaat
     1621 cgcaccaagc gtccagcttg ctgatatatc tgtctgaatt atcgccctga ctttggcact
     1681 tatgctggat aaacatccac ttaaaaacaa tgacatccgt tcatctgttt caaacagaga
     1741 caggcaggcc atcataagaa acatatccgt gacctctccg gaaaatccct ggctggtaat
     1801 taaagccgca gccaacgcag gattgttggg ttccagcaac aggtaaagcg gaatgtcagt
     1861 cagacgagtc ctcgtcagct tatgctgact ttccagatat tgacttacga cggattcgct
     1921 tatatcgaca attttaactt tgccataatg cataaggaaa agctccctga tgcatttggt
     1981 ggccagaacg actgagcctg gcttaagtga caacgtatcc ttttcaagaa aaatattaat
     2041 tggggagca
//
LOCUS       DAZFBR010000286         2037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFBR010000286 DAZFBR010000000
VERSION     DAZFBR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.289"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..374
                     /locus_tag="ACHJZ2_005139"
     CDS             <1..374
                     /locus_tag="ACHJZ2_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571039.1"
                     /translation="MQKKLKQTSVPDFIYYSIEKHNKYPIYVLHMPSNNIIEIGYNII
                     NTDLVIGEKIHFRTLSNRSLYFNLTHPPLIATIKDDVFCTLHDYYNHNNETKSTIDNY
                     ISKIKDNHNTPWLLNNENVQE"
     gene            complement(1246..1386)
                     /locus_tag="ACHJZ2_005140"
     CDS             complement(1246..1386)
                     /locus_tag="ACHJZ2_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4571040.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1688..1951)
                     /locus_tag="ACHJZ2_005141"
     CDS             complement(1688..1951)
                     /locus_tag="ACHJZ2_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO4571041.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
BASE COUNT          637 a          472 c          369 g          559 t
ORIGIN      
        1 agatgcaaaa aaaactaaaa cagacgtctg tgcctgattt tatttattat tcaatagaaa
       61 aacacaacaa atatcccata tatgtattac acatgccatc aaacaatata attgaaatag
      121 gctacaatat aataaacacc gatctggtca ttggcgaaaa aattcatttc aggacactaa
      181 gcaatcgttc tttatatttt aacctcaccc accccccatt aatcgccaca ataaaagatg
      241 atgtgttttg cactcttcat gattactata accacaataa tgaaacgaaa tccactattg
      301 acaattacat atcaaaaata aaggataatc ataatacccc atggttatta aacaacgaaa
      361 atgttcagga ataaaaaaaa agagacaatt cccgagataa aacaaaagag cataaacata
      421 tgaaccggaa attctatttc aaaaatatcc tgtggggaat gtgccccatt ataatatcca
      481 tattgcatct gatatatgac acaccaacag aagcatcgat aaaagttgac ttatttctga
      541 tactcagtgg cctggccctt ccttgttcaa tatacctgac aaaaacaatc acactaaaat
      601 acacatccat atcattctgg aaaaaacctc ttttctggtc ttatggtata tatcattttc
      661 atcttgttta tataatgata tgtatagttt tagcaatacc ttctgcaatt atcgttttat
      721 tctttcactt ttatcaaaac atcaccagaa actggaagtg acaaccgcaa taatgaacat
      781 aaaaaggcaa atcagaatgg cagtaaatca ataaagtacc atttcatatg aatcatcaga
      841 aaaacggtgg acgcgacatc cagcacgagg caaaacacag caacaaaata agccggatat
      901 cgcgccctgt acttttctaa ctcaactccc ggcattgtgc aagttttaat cgcaggtgga
      961 ttcagagcca cgcccccatt gattcagcct gaagacacgt atatccttgg agaaagcagg
     1021 aatctcattg ctgtgaaatt ccttacagaa tcttcagccc cattttatgg ggcttttttt
     1081 ggtgccccca cgctgttacc acactttcgg gatgcgaata aaaaagccca cagtttttac
     1141 actgcggact tttgtttttt ctttgtgatt tgtgtgcaag ggtgcgactt tcgaccgaac
     1201 ctttggcaac cggatagcgg ggactgttgt ccccgctgcg gtttcttacg gtttacactg
     1261 caagaacgcc gcaatttccg accacatgaa gcttaccctg aattcacacg gtgatgcgtg
     1321 taacacccag acggtcagca acatggtcag gcataacatg cccacaatga tcgttttttg
     1381 cggcatagca cttgctcctt ttacagagag gcgctaacct tccacttgct gaaggtagat
     1441 ggtcagggcc tcgggttaac gtttaagttg actcggggcc tttccacatc cggccttcag
     1501 gtaatccttc cagccgtcag ccgaaaggca cccgcgcgta atgtactggt tttcagagag
     1561 gtccgcaatc ttccggtgac gagatcatct gcacacgccc cggtgggacg ggctaacggt
     1621 atattcaggt gcaggatctc tctgtctggt gcgtttttaa attgtcaaac gcaccctggc
     1681 ggcaaaatca gccacctttt ttcgcatcaa caaaacgccc aaaacgctgg gtaaagcgtg
     1741 caacattacc ttctgaagcg acagttctca gtttacctgt ataaaacgga tgcgacttag
     1801 aagaaacgtc aattgtaaca tatgggtaag ttacaccatc cagttcaatt acccggtcag
     1861 ttttgatcgt cgaaccgatc ctgaagtact catcaatact ggtatcgtga aatactactg
     1921 tacggtattc cggatggata ttcggtttca caacagtttt ccccctttaa gtgaaaattc
     1981 cgcataaagg tacatattca gacggtcaga ttcaactgtc tgttcttata acctcac
//
LOCUS       DAZFBR010000287         2037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFBR010000287 DAZFBR010000000
VERSION     DAZFBR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.290"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            18..161
                     /locus_tag="ACHJZ2_005142"
                     /pseudo
     CDS             18..161
                     /locus_tag="ACHJZ2_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(290..366)
                     /locus_tag="ACHJZ2_005143"
     tRNA            complement(290..366)
                     /locus_tag="ACHJZ2_005143"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(330..332),aa:Arg,seq:tct)
     gene            complement(380..456)
                     /locus_tag="ACHJZ2_005144"
     tRNA            complement(380..456)
                     /locus_tag="ACHJZ2_005144"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(420..422),aa:Arg,seq:tcg)
     gene            complement(466..541)
                     /locus_tag="ACHJZ2_005145"
     tRNA            complement(466..541)
                     /locus_tag="ACHJZ2_005145"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(506..508),aa:Ile,seq:cat)
     gene            complement(582..707)
                     /locus_tag="ACHJZ2_005146"
                     /pseudo
     CDS             complement(582..707)
                     /locus_tag="ACHJZ2_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(983..1417)
                     /locus_tag="ACHJZ2_005147"
     CDS             complement(983..1417)
                     /locus_tag="ACHJZ2_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4571042.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(1503..1643)
                     /gene="ylcG"
                     /locus_tag="ACHJZ2_005148"
     CDS             complement(1503..1643)
                     /gene="ylcG"
                     /locus_tag="ACHJZ2_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4571043.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1640..2002)
                     /gene="rusA"
                     /locus_tag="ACHJZ2_005149"
     CDS             complement(1640..2002)
                     /gene="rusA"
                     /locus_tag="ACHJZ2_005149"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO4571044.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
BASE COUNT          521 a          552 c          454 g          510 t
ORIGIN      
        1 cccctccgga gaggggcatg tttgcatgca cattcttttt cttgcatggt gccgggtgcc
       61 tcccggtgaa ttcagtatca gcacctgaat ccgcgattat cacatatacc tggttgctga
      121 ttgcccctcc gcacaggggg attcaccata cgaaattttt ttaacaaacg ctcagcatgt
      181 caggcaacat tcaactgcct gaattgtgag gcatttaaca tttcactgtc cggtgtcttt
      241 cctgtaataa aaagcccgca aaagagagtc agggcagata agtgtggtgt ggcgcgttgt
      301 actggattcg gaccagtgac cgattgctta gaaggcaatt gctctgtccg gctgagctaa
      361 caacgctgaa taccgataat ggaccgccat cggggacccg aaccccgcgc aaccagcttc
      421 gaaggctggc gctctgtcct gatgagctaa tggcggtatg tgatatggtg gcccttgctg
      481 gatttgaacc agcgacctgg cgattatgag tcgctcgctc tcaccactga gctaaagggc
      541 cgggcgcagg ataataacgg tacgtaacta atcctgcaat atcatccgtt ctgactgact
      601 aaatcctgaa cttccctgac cgtctgctca aaacgttcag tctccagctc aacgccaatt
      661 gcacgacgcc ccagcgacat tgctgctttg acgctacgga cataaaaaag ccagccactg
      721 ggggaggctg gcaaactcgt agagcaaaat gctgttacgc aaacttcgtt acagggtcat
      781 cctgcaatac aaaaaataca caatatttag aaaactaata gtgccatgtg caatttttaa
      841 gattttgtta ttaattgtgg tcgcaccttc ctttctgtgt actttccgta tagctcacag
      901 gattctgggt acaaaaaaac ccgcgcatcg gcgggttaag cagcgtggca atgtaaccac
      961 tcttatcatg atatgcagat ttttacgatc gtaaactatt ttttcgctga taaaatacag
     1021 aggttctccc tcccggcaat tcacgctcaa cataccgatc catctcaagc ctcactccca
     1081 gcatcatcag catgccttca acaatcccct ccgctttgtg aaggcgttta cctatacagg
     1141 tgtcagagca cccatgtttc cgtgccagcg ccatgaacgt ctcccccaac acgtaataat
     1201 caaccagcaa gtcatgcaga tcgcgattgt tccggtaaag gcgggctata cacccgcata
     1261 tcaccatcgc atcatcgtca cagcactgcg ggcgtgattt tactttttcg gggatcagtc
     1321 ccttaaaacc ggcggcaatg gacgaccagg tcacatcttc atgattatta gccgcccacg
     1381 ctccccaacg ctcaagaacc atctgaatat cacgcatcaa cttactccac aaaaatcaga
     1441 ccagaacgcc aattacaagc aaaaatcaac aaaacagtat tagttgattg ttatctctga
     1501 cttcatactc ctgctcctgt cagggttttg gcgtaattct tcagtattcg gtaatcggtc
     1561 aaaacagaac cggggaaacg atataagcgc agacgccccc agcggtggcg aagaagttct
     1621 gccatattaa actcaaacat cattcattcc ccatttcggt gatggtcagt tccagcctcc
     1681 cacctttggt aacaggcatc ttcacaacgc ggtaatcaac gacctgagca tcatccagcc
     1741 agaaacctgc tttagtgagt gcgtcaaaag cggctttttg cagattatcc aggtcacggc
     1801 gacggcgatc cggcatgtgg cactcaatgc ggattttcac aggcatagcc aggccgatat
     1861 ccagcattgc gtttttaatg attcgggcga cgttatcgcg gtatgcctgc ccctctgcgc
     1921 tgacgtgcgt gcgcccgcga ttatggcggt aatagcgatt attgctcgga ggccagggta
     1981 atgtgatgct gtaggtattc acgccttgat taccccctct ttcagccaga taacctg
//
LOCUS       DAZFBR010000288         2023 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFBR010000288 DAZFBR010000000
VERSION     DAZFBR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.291"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            21..611
                     /gene="espM1"
                     /locus_tag="ACHJZ2_005150"
     CDS             21..611
                     /gene="espM1"
                     /locus_tag="ACHJZ2_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFO4571045.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYI
                     ANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            complement(830..1886)
                     /locus_tag="ACHJZ2_005151"
                     /pseudo
     CDS             complement(830..1886)
                     /locus_tag="ACHJZ2_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          543 a          410 c          486 g          584 t
ORIGIN      
        1 tttaatgaaa aggatttgtt atgccagtaa atgcgacagg tgtgtccttc agctcttttg
       61 gtatcagtta tcataaagat aattctttcc ggggcaccat ccgtgggaag aatgatgagg
      121 tcgtgaagtg ttcaatggga gagcgctcta tccgctttaa tgttaacaaa tttagcggct
      181 gtatactgga gacggtaagc aggcagagta ctaaagatat tcatggatgg gtaagtgatg
      241 aacggacagt atatccgtca agggttatca accaagagat tgataattgc tgccttcaaa
      301 aaaatgcaaa aatttcttct gaagagagga aaatggtctt ttctcttgta agtaaggagt
      361 ttgaactaac tcttgatgtt aaagcggcac aaagttctat taatcatata ataataggaa
      421 atgcttcttt tggcaaaaaa atggatgctc tttgcgatgg tatgagccga gctgtaaaaa
      481 acagtacaac agattacata gcaaacgtgc ttgcagacaa gttttatcag aaacatatcg
      541 ccccgggtgt tgatattgta aaactacgaa atgaaatccc aggttatatg agtcgtgtta
      601 tacaggggta aggttggagg aggctatgcg ccagtatcat tggttttcat cctgtaaaaa
      661 gatatgagta aaatatacaa caggcgttac actatatgta tgtatccaaa taactcatag
      721 ttcacctgta cagttataaa tcgtaaagaa aattgcagcg cgtcgtacgc agaaacgtgc
      781 tgtgattgac tatttatagc tttgtcacaa cgacaagatt cagtagtgtt tacacgggcg
      841 ggagaaatga cggaataact aaatagctgc gcctaatatc gcacattttt gccagcccat
      901 gtttgccttc ggggaatgga ctgacggatt ccataaaatg gcgaactttt tcaatagttg
      961 ccacattgag cggcactgat gattacgcgt tattgtggcg tgaagtatct gccatagcta
     1021 ttcaaaatga ttcacccatg gagattaaac cggctgataa atgactctgc acttcggttt
     1081 atccttcagc cagcgctggg tttcccggcc tttgtggata atgtagttgt cctcgagcag
     1141 cgtgatggtt ttcgtccgac agtatgtagc tttaagccgc ttcagcagtc tgatgaacag
     1201 cgccgaactt ttgctgttgg cgcccacaca gctgacttaa cctgtcccgc tgtgcagcgc
     1261 tacgtccaga gaatattttt cattttgttc cggcgtgacc cctcgttttt gcttcccgag
     1321 cagtttccag tccgcaccga ttttgggatt aagatggata aatacttcat cttcataaaa
     1381 gaccggatgc tctgtgctgc attcgtccag tgcgttatgg attgttgcca tctttttatc
     1441 tttaggcggg gcacggatac gtagagttgg cgcagccctt cgccacacaa gccccacaga
     1501 cgacaaccag cggcgaatgg ttccagcatg taacaggcag ccggttatct cattgatttt
     1561 tattgtcagc agttctgtac tccagcgtga ataattgtag ccaaagtcgc gagtgaatat
     1621 tttacctgtt cacgtaatag tgtgtagata tgcgcaaacg gccagcggcg ggctcgctcg
     1681 gcaggtaatg atttcagtcc ctcaacaccc gactgtgtga actagttaat ccagcgccca
     1741 acagaggaac gggcgcagca tagtgtgcgg gcaacgtcgc tgacacggtc gccccggtgc
     1801 agcatcagca tggctgtcag ccttttatgg atggctttct gtattaggcg tcgttcgtca
     1861 cgggaaattg gtggtatgat cgacattgct cagtccggtt ggtgatttgt attgatttgg
     1921 cgattgatca gatcgcacaa tcgggttgag ttccctctaa atgatctact attctgcgca
     1981 gctatttagt gagtatatat tttatgctta taaattagat taa
//
LOCUS       DAZFBR010000289         1960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFBR010000289 DAZFBR010000000
VERSION     DAZFBR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.292"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            255..470
                     /locus_tag="ACHJZ2_005152"
     CDS             255..470
                     /locus_tag="ACHJZ2_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4571046.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            475..819
                     /locus_tag="ACHJZ2_005153"
     CDS             475..819
                     /locus_tag="ACHJZ2_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO4571047.1"
                     /translation="MKKKYELGVKGINNYPDKITVTVAPEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            870..1403
                     /gene="rrrQ"
                     /locus_tag="ACHJZ2_005154"
     CDS             870..1403
                     /gene="rrrQ"
                     /locus_tag="ACHJZ2_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4571048.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALEWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1559..1741
                     /locus_tag="ACHJZ2_005155"
     CDS             1559..1741
                     /locus_tag="ACHJZ2_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571049.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            1754..1885
                     /locus_tag="ACHJZ2_005156"
     CDS             1754..1885
                     /locus_tag="ACHJZ2_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571050.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
BASE COUNT          480 a          396 c          582 g          502 t
ORIGIN      
        1 ctggctaaga aggatagttg ggtttcacat gatacttata tctggcagta cattttctga
       61 cagacagtga tgggtgttgt caagatattg tgtcatttat aacctgaatc agggggggga
      121 gccggaatgt tatctggcat ttttagcaga gcctgaatgc cataatcacg gctcccggcg
      181 ttggccgtca gtgggcgaca ctggcggctt tttgttttcc tttactttca ttttctgtcg
      241 gcggtgacgg agacatacat cagatggaaa aaatcacaac aggtgtgtca tacaccacgt
      301 cagcggtggg gacgggatac tggttactgc agctgctgga caaagtctct ccgtcccagt
      361 gggtggcaat cggtgtgctg gggagtctgc tgtttggcct gctgacgtat ctgactaacc
      421 tgtatttcaa aatcagagag gaccgtcgta aggcggcgcg gggggagtaa agcgatgaag
      481 aaaaaatacg aactgggtgt taaagggata aataattacc cggataagat tactgttact
      541 gtggcaccgg aaattggtgg gtatccgtca ctgttgttgc cagatgtggc gattagtctt
      601 gaccgtactg aaggtgccac gctggagttt tacgaagctg aggcgaaaaa gcaggcgaag
      661 cagtttttca tggatgttgc tgccgggtta tgtgaagggg atggtccgtt gccggaaaag
      721 cgccccgtaa ttttagaggc gcaggatgtg ttgataacct acagaggaaa actaccggga
      781 ataattacgg gttctctgaa gactccaccg ctggcctgaa gacttaacat atccagggat
      841 ttgaaatcga taaaccctga taaatatcca tgaacgcaaa aatcagatac ggcctgtcgg
      901 ctgccgttct ggcgctgatt ggtgcagggg cgtctgcgcc tgaaatcctc gaccagtttc
      961 ttgacgaaaa agaaggtaac cacaccacgg cataccgtga tggtgcgggt atctggacca
     1021 tctgccgcgg tgccatcctg gtggatggta aacctgtcgt tccgggcatg aagttgtcga
     1081 aggaaaaatg cgaccaggtt aacgccattg aacgtgataa ggcgctggag tgggtggagc
     1141 gcaatattaa agtaccgctg accgaacccc agaaagcggg gatcgcgtca ttctgtccgt
     1201 acaacattgg tcccggtaag tgtttcccgt cgacgtttta cagacgaatt aatgcaggtg
     1261 atcgcagggg agcgtgtgag gcgattcgct ggtggattaa ggacggtggc agagactgcc
     1321 gtattcgctc aaacaactgc tacggtcagg tttcacggcg tgaccaggag agcgcgctgg
     1381 cgtgctgggg aattgacaga taagcagaat attttgctga aaaatgcggt ttgctcacac
     1441 tggcggataa cacgaaatcc tgcgaactgg caaaaactaa gtgaataaaa gtaaaacccc
     1501 gtttgttggc cgcaaacggg gttttgtgtt tctgaccttg agtaaggcaa gggagaacat
     1561 gggaaagtat aaacgaattc tgttgagatt gactatgaaa aacggccttg aactgaaagc
     1621 acctgtaact gatgacgtca gcagagcgct ggcttttgct attaagtggg tggcggtcgg
     1681 tattgctgtg tctccgatgc tgtatgggct ggcaaaactg gtcattgcgt tgaaatcgtg
     1741 aagaggatta agcatgtcag acaagctcat aacgctggcg aagatcctct gtgtaatcgt
     1801 cggcatttca ttttcactaa tgctggttgc tatttgcttt tccactgcct ggcgcgtctt
     1861 aactttgtca tggctggtgg ggtgaggggg atatgaaccg tgttctgtgt gtggtgatta
     1921 ttgtcctgct ggtagcctgt ggtgtgctta gtctggggct
//
LOCUS       DAZFBR010000290         1944 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFBR010000290 DAZFBR010000000
VERSION     DAZFBR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.293"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>1944
                     /locus_tag="ACHJZ2_005157"
     CDS             <1..>1944
                     /locus_tag="ACHJZ2_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO4571051.1"
                     /translation="QTQKIKDIVDFLGIQDNTIKVKLESDIKPISEIQQPLHSILSRQ
                     KEHVKNLLSGLLDEFSNKLRKQGLSLKTNVLSVNNFKESKINSDTVEVTVTDLQGRLY
                     RVDIDTRVIGLTFKEGINSLSEALEHMNIDAIMSVIGLVQYARMIKMNDNISAIDHAG
                     AVSDIKNIVDKFLGGILTLTNNRVYNPGGVSGASLEGFISSGLEVCASRMGGTAGRYL
                     SNVAKVIKLPLLDIGINIWSLYDSSLNHAKATTQIEYISTAIDVSFSSINTALSIGAI
                     AYPPLAIAIVPITIFSHEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVLSINKATG
                     IIDLSNNQVLGNIYLDMRENPPILHGEKSYNSGKNIGSHPDMTDREIMESRAYNFACT
                     KLSDAGEPDIFGWGDKEICNSMELSESQLANGYSNRQWPSQIPVIPEGIYNTVYLGYG
                     ETLRANTEVTLSSTGYFYEIARAYTDDELSEPLLTVCNQHSHVIGGKEPLTIIIPAIE
                     HGMLGSNLHMIERFKNYNFSISGGKGGIKLMVGGIGDYNIECTPGVRNIISFEQLSRD
                     FNLDLDLSDGRKQNFNFHHPSGFYSGKVMSITQKGINAVVGTKLGYDKIRGNNLDNTF
                     SLGSGGGVIYSGGGSNTYFVPATL"
BASE COUNT          662 a          290 c          391 g          601 t
ORIGIN      
        1 caaactcaga agataaaaga tattgtggat tttttaggta ttcaggataa cacaataaaa
       61 gttaagcttg aaagtgatat taaacctata agtgaaatcc aacagcccct tcattccatt
      121 ctttcacggc agaaagaaca cgtaaagaat ttactatctg gattgcttga tgagttttca
      181 aataagttaa gaaagcaagg tctttcactt aaaacaaatg ttctttcagt taataacttc
      241 aaagaatcaa agataaatag tgatacagtg gaggttacag taacagatct gcaaggaaga
      301 ctttatcgtg ttgatataga tacaagagtt attggcttaa cttttaaaga ggggataaat
      361 agtttatccg aagcactcga acacatgaat atcgatgcga ttatgtctgt tattggcttg
      421 gttcaatatg cacgaatgat aaaaatgaat gacaatatta gtgcaataga tcatgcagga
      481 gcagtatcag atataaaaaa tattgtagac aaatttttag gcggaatttt gactcttact
      541 aataatcgcg tatataatcc tggcggtgta tctggtgcct cgcttgaagg atttatatct
      601 tctgggttgg aggtgtgtgc ttcaagaatg ggtggcaccg cagggcgtta tcttagcaat
      661 gtggcaaaag taattaaatt acctttgcta gatataggca taaatatttg gtcattatat
      721 gattcttcat taaatcatgc taaagccacc acacaaattg aatatatatc aacagcaata
      781 gatgtatctt tttcatctat aaatacagct ttgtcaatag gcgcaatcgc atatcctcct
      841 ttggcaatag ctatagttcc gataactatt ttttctcatg aagtaaaaaa ctatgctgtt
      901 tatgtgaatc aaattaatga gcgccataag ttatggctcg aagctgaaaa gtatctggac
      961 aatggaagtg caaaggttct aagcattaac aaagccacag gcataatcga tttatcaaat
     1021 aatcaggtgt taggtaatat ttatttagat atgagagaaa atcctccaat attacacggt
     1081 gagaaatcat ataatagcgg aaagaatatt ggtagtcatc ctgatatgac tgacagagag
     1141 attatggagt ctagagcata caattttgca tgcacaaaac tctcagatgc aggtgaaccc
     1201 gatattttcg gttggggaga taaggaaata tgtaattcaa tggaattatc agaatcacaa
     1261 ttagctaatg ggtattctaa ccgccaatgg ccttctcaga ttccagttat tccagaagga
     1321 atttataata cagtatatct tggatatgga gaaacattgc gagctaatac tgaagttaca
     1381 ttgagtagca ctggttactt ttacgagatt gcaagggcat atactgatga tgaactatct
     1441 gagccattat taacagtatg taaccaacat agtcatgtta ttggtggaaa agaaccatta
     1501 acaattatta ttcccgctat tgaacatggt atgcttggct ctaatctaca tatgattgaa
     1561 cgttttaaaa actataattt ctcgatatca ggagggaagg gagggataaa attaatggtt
     1621 ggtggtattg gtgattacaa catagaatgt acccccggtg ttagaaatat tatatcgttt
     1681 gaacaattat cacgtgactt caatctggat ttggatttat cagatgggag gaaacaaaat
     1741 tttaattttc atcatccctc aggtttttat tcaggaaaag ttatgagcat aacgcaaaag
     1801 ggtataaatg cggttgttgg tactaaactg ggatatgata agattcgtgg taataatctt
     1861 gataatacct tttctcttgg gagtggagga ggtgttatat attctggagg agggagcaat
     1921 acctatttcg ttcccgccac acta
//
LOCUS       DAZFBR010000291         1936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFBR010000291 DAZFBR010000000
VERSION     DAZFBR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.294"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(36..401)
                     /locus_tag="ACHJZ2_005158"
     CDS             complement(36..401)
                     /locus_tag="ACHJZ2_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4571052.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
     gene            443..628
                     /locus_tag="ACHJZ2_005159"
     CDS             443..628
                     /locus_tag="ACHJZ2_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4571053.1"
                     /translation="MSSKNRTRRTTIRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            complement(758..898)
                     /locus_tag="ACHJZ2_005160"
     CDS             complement(758..898)
                     /locus_tag="ACHJZ2_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571054.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(1255..1479)
                     /locus_tag="ACHJZ2_005161"
     CDS             complement(1255..1479)
                     /locus_tag="ACHJZ2_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571055.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(1544..1750)
                     /locus_tag="ACHJZ2_005162"
     CDS             complement(1544..1750)
                     /locus_tag="ACHJZ2_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4571056.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            complement(1754..>1936)
                     /locus_tag="ACHJZ2_005163"
     CDS             complement(1754..>1936)
                     /locus_tag="ACHJZ2_005163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571057.1"
                     /translation="WRLAVVRCGWQQTITVTTRSPTKRSAIKKPESWNRRMPPLLTCR
                     CASVMLLRSMQNTRGN"
BASE COUNT          533 a          523 c          427 g          453 t
ORIGIN      
        1 tttttttcgc cggatgatcc cccccccggg gcgggtcaca gtccgatgat atcgtctgcc
       61 ctgccatgac ctccggacac ctccggcagc gtcgggtccg gcataccacc cgccgcttca
      121 cgagcagact tttgtcgatg gcattcggta cagagcgtcc agagattcgt ctcctcatta
      181 ccaccaccga actgaagtgc aattcggtga tcgagttcac tgtcacagag gtcaaccaca
      241 cgaccacaga gacagcactg cccggcatcc ctgagccaga tatgacgctt gagggaaaca
      301 cgtgcactgc cactgacacg acgctgttca cccttcagaa tattcacccg ccgggtgttc
      361 agagttttga ttctgcccgg taacgtacga agcacagcca tgtaaaatcc tcgccatata
      421 gcttgtcacc agaggaaaga aaatgtcatc gaaaaaccgg acccgcagaa ccacaatccg
      481 caatatccgt ttccccaatc acatgattga acagatcaat atcgcccttg aacataaagg
      541 ttccggtaac ttttcagcgt gggttattga agcctgcaga agaaggctgt caacagagcg
      601 ttcgggtatg aattacataa ttaagtaaca tggtgttcac agaacacaca gttaccggac
      661 acatcagttt tccattcgtc ccccggcagt acaggcttcc cgtctgacgg gatagcctga
      721 aaaaacacag aaaattattt gttataatta atataactta ctcaaaaaaa agcgacgaga
      781 aaatcagcat caacgagcaa taagcgccaa tacgtgataa caaatggcag ccatatttat
      841 ctgcagtata agcaatggac aggataacca caccagaaac cgtcagcata aaatccattt
      901 gaacttcccc ggacaaaatc gactcatcta aagatttaca gctcttttta ttatcaatat
      961 gttaaaagta aaataaacag atgttcaata atacgaatac aaaaacgtgc tgaaaatcaa
     1021 tgaatccatt tctgtgttat caattaatag tgataaacat ccggcttctt ccaccatcgc
     1081 accggaccag cgaccatgag gggacaacgc cgcgctccgt taacgcggta aaccccggtg
     1141 tgtatcgttt ttgattatcc ccgcacactc gcgcagagga ttctcccggt cgggctgcgg
     1201 tctctgttaa tgcaggaata cggcgacaat accgcgcatg aataataagg tcgctcaaca
     1261 cactggctgt aatgcagcgg ataccatgcg gcatttagcg gcattcatcg tacactcaac
     1321 ggttagctct tcattcgtgg cattcacctg aaaggtcctg gagtgtaatt gcgtacattt
     1381 accactgaac gaaccttcaa caagaacacg accacgctgc aaaatacgga acggaattgt
     1441 tccctgaaaa ggctttacgg ttaccaataa tttcttcatg cattctccga ataacaaaaa
     1501 tactagttaa tacactgagt gcggatatat tcctgaagca ttctcaatgc tgcctggtcg
     1561 ctgatgattc cgtctctgat accgagaacg tttcgtccag caaccggaga gagttcgacg
     1621 gcggcatcat tgcccacgcc ggaggtgccg gtggcttcac gcacggtacc ggggcaggtg
     1681 gcgttgatcc gcaggcgctt acgaccagcg gcaacatcag cacgcagagt ttcattttca
     1741 gctctcgcat cggctaattc cctcgagtat tttgcatcga gcgcagcaac atcacgctgg
     1801 cgcacctgca tgtcagtaat ggtggcattc gcctgttcca gctctctggc ttttttatcg
     1861 cgctgcgctt tgtaggtgag cgcgttgtca cggtaatggt ttgttgccag ccacagcgca
     1921 ccacagccaa ccgcca
//
LOCUS       DAZFBR010000292         1932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFBR010000292 DAZFBR010000000
VERSION     DAZFBR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.295"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(246..524)
                     /locus_tag="ACHJZ2_005164"
     CDS             complement(246..524)
                     /locus_tag="ACHJZ2_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO4571058.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETETNNTNKS"
     gene            952..1098
                     /locus_tag="ACHJZ2_005165"
     CDS             952..1098
                     /locus_tag="ACHJZ2_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571059.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
     gene            complement(1235..1882)
                     /gene="nleG"
                     /locus_tag="ACHJZ2_005166"
     CDS             complement(1235..1882)
                     /gene="nleG"
                     /locus_tag="ACHJZ2_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFO4571060.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
BASE COUNT          572 a          357 c          389 g          614 t
ORIGIN      
        1 ttttcgtgtc tttaagtgat accagatggt attgcgctaa atgtaccaat gggtatacca
       61 agagcactaa aaacaaaggg gcaaccactt tgtaacccct tgattatttg cagaagcgca
      121 gaaaatcgaa ctctggaacc ctttcggggc atcctttttc aagaatgaag aaaaaaataa
      181 ctaaaaaatc aagttgttag gaagttattc atggctagtg taccaactct tttctgggct
      241 aatagctatg atttgtttgt attatttgtc tctgtctcta ttatttttga catccatgcg
      301 acttctgata ttgataacct cagttgtgga gttctgtctg cgcttccggg aggacgcata
      361 tcaaaactac aggatcttct gttaaacact gtaaagccat ctacattgca tgttgttgag
      421 tctaagatac aatttttagt agatgattta tctgctatag gcctagatat ttcaggttga
      481 tgccaagtgt gtcctgaaaa aatacttttt attgaaaatg gcatttttat cctcacatta
      541 tgtctttctc ttatagtgca atttaatgta tttagaaaca ttaaactatg aatctaaaaa
      601 ggctacgcag agtactgtta cactttaaca aatcatattt accactctgg aaatgggatg
      661 gctggttcat aaaataatga gtctttttca acggttgtta cattggtgta ctgatgatta
      721 tgccttgtcg ctaagagcta ccataaccgt acagcgtgat tcatccatgg cgaataaatc
      781 gactgataaa tccccctgag tttcagctct tgctgttgga ccttgcccgt ttcgctatgg
      841 aacgcgtacc agctagagaa tattattcgt tctcctccaa tgttacgcaa gttttgtaaa
      901 cggtaaccaa tcgtgattag tcctgcatgt aacggacatc aagctgtctg gttgattttt
      961 attgccagta gttcgagaga gcagacaact gcgtccgatg gaaaaatggg cacgacaggg
     1021 tattcaggca acctcgctga ggaggcttat ggtacagatt gtaatgtgaa gtataaaacg
     1081 ttaaaaattc cattttagaa actaaatagc tgtaatataa gttcgatcta atctactatt
     1141 ttgacagaca gattgtatca agaattaggg agtatgaaat agatggcaat aactagtaaa
     1201 attaattttg caacatttaa atcgaaccat acagtcaaat tctagtgcat atattttgtg
     1261 tggctaatat gcaaaaatat tgttctatta gattaaaatg gcattcatct ttgctgacaa
     1321 tcatttccgg aacaaagggt tcacggctga gagggtgtgg agcgttgcgt cggataagtt
     1381 cagtgagtgc attgtggtca tataatgaac atatttctga gttttttgca tttctgacaa
     1441 aaacaccagt ttcaggaatg cataatgtta tcgggcaatt caggtgttgt tcatgacagg
     1501 aaaagtcttg atgactaaca gtaaatgcat gagagttaat tttgttttgt attgcactac
     1561 tgacgttttg gcgcgtacat ggggttgcac ggctctgttc catatataaa cggaatgcct
     1621 gcaaaaaagt atggcctctg ttaagctgta tctccagatt ttctgcgaga aggcgatgcc
     1681 ctcttgcact aaggcgatcc aacagtccgc cggaaaccgg ttccacgcta aaactgtcca
     1741 taaatgaaat atgatgaagt ctcatagtgc ggcctctcac aacaagctgt tcactctgac
     1801 taactcttcc gacatgacgt aaagcctcca gttcactttc agacaatact ctttcactcg
     1861 aaaagtttaa tatgacaggc atattttctc cctctttagt ctatgttatt gaaccatatt
     1921 ggtgacagga gt
//
LOCUS       DAZFBR010000293         1922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFBR010000293 DAZFBR010000000
VERSION     DAZFBR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.296"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(445..1422)
                     /locus_tag="ACHJZ2_005167"
                     /pseudo
     CDS             complement(445..1422)
                     /locus_tag="ACHJZ2_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1800..>1922)
                     /locus_tag="ACHJZ2_005168"
     CDS             complement(1800..>1922)
                     /locus_tag="ACHJZ2_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HFO4571061.1"
                     /translation="LKAVKSEMIIAREMGEIFSYMPGEIDSYMKYINNKLSKIE"
BASE COUNT          485 a          435 c          414 g          588 t
ORIGIN      
        1 gtgctaatgt gataaaaaaa atatttatct cgatgctacc caacatacga ctcatttatt
       61 tttctcggtt tctccttgta tatatatgtt gctttataat tttactacgc acgtcaggcg
      121 tatctgtaac atagctccgc attatgcaat gcgactttgc atacaaatag gaataaatct
      181 aatagctgta gatttgctcc tgtatttcct gaaccaagcg agccagtaca tgatgcatat
      241 gtctggaaaa taattcagag taaggataag tccagctttg ttgaataaat caggtttgga
      301 ataagccccc tcagttggta gctactgtca ggtaatacgc acgtattatg acattcgtgc
      361 cctcgtcatt ttgtttagta cacgcactgt agtggcacag aacttctgcg acctgaccat
      421 cctaaatagc tgcgccgaat accgctacac ttttaacagc ccatatttac caccggggaa
      481 tgggctcacg gtttccataa aataatgaac atttttaagc agttaccaca ttgaacggca
      541 ctgatgattg cgcgttatcg tatcgtgaag cgcctgctat aaccgctcaa catgattcac
      601 ccacggcgag taaactggct gataaatcac tctgaacttc ggattttctt tcagccagcg
      661 ctgtgtttcc cgacttttat ggatgatgta gttgtccact atgagcgtga taggtttatc
      721 ccgtcggtat gtcaatttca gatgtttcaa cagactgata aataaggctg agcttttgct
      781 gttgccaccc acatagctga ctttgcccgt tccactatgt aaagcaccag ccagatagta
      841 tttttcattc tgccccggtg ttaccacatg tttttgctgt ctgcgaagtt gccagtccgc
      901 accggttttg ggattaagat agatatacac ttcatcttca taaaagaccg gatgctccgc
      961 actacattcg tccagtgctt tgtgaattgc tgctttatgc gggttacggg tacgcagggt
     1021 tggcgcagcc ctgcgccaca caagcccgac taatggcaac cagcggcgaa cggttccggc
     1081 atgtaactgg cactcggtta tctcgttgat ttttattgcc agcagttctg tactccagcg
     1141 tgaacgctga tagccaaaat atccgggaga gtgttttatc agttcacgaa acaatgtgca
     1201 gatatgctca aacggccagc ggcgggaacg tcctgcgggt aatgatttca gaccttcaac
     1261 actcgacagc gtgaaccagt taatccagcg cctgacggag gaacgggcgc agcagagcgt
     1321 tctggcaacg tcactgacgc gagcgctccg gtgcagcatc agcatggcgg tcagcctgcg
     1381 ggcataattt ttatcgtgag ttttatagat tgctttttgc atcaggcgtc attcgtcgcg
     1441 gggaattggt gctatgatcg gcatcgctca gtccggttag ggggggattt ttggcgattg
     1501 atcagatcgc acaatccggg ctgagttccc tttcattgat ctactattcg gcgcagctat
     1561 ttagttggtg attttgaact tctgctttgc tactaagcag cctgcgtttt caggaaaata
     1621 cgtgatgtga tcctgcaact cagcaacagt tcggttgatt caacaaagcg ttttttaaca
     1681 ataattcttc attgtaaaaa gcatcttcag ttgtgatcaa atattattat tcttattggt
     1741 gttctcatat aatgaaatag aattaatttt agccccccta cacaagtggc tgagcttctc
     1801 tactcaattt tagaaagttt attatttatg tatttcatat aactgtctat ttccccaggc
     1861 atgtagctaa agatttctcc catttcacgt gcgataatca tttcagattt tactgccttt
     1921 aa
//
LOCUS       DAZFBR010000294         1899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFBR010000294 DAZFBR010000000
VERSION     DAZFBR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.297"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..158
                     /locus_tag="ACHJZ2_005169"
     CDS             <1..158
                     /locus_tag="ACHJZ2_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571062.1"
                     /translation="IIIDSEYKSNKAVKEYVEILKEFNICPEKVAEYLSEFSGKHFLD
                     VYNYYSN"
     gene            340..525
                     /locus_tag="ACHJZ2_005170"
     CDS             340..525
                     /locus_tag="ACHJZ2_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO4571063.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRIIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            766..1755
                     /gene="nleB"
                     /locus_tag="ACHJZ2_005171"
     CDS             766..1755
                     /gene="nleB"
                     /locus_tag="ACHJZ2_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO4571064.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFLSLENEYQNIKIIYRADVDFSMYDKKLSDIYLENIHKQESYPASERDNYLLGLLRE
                     ELKNIPEGKDSLIESYAEKREHTWFDFFRNLAMLKAGSLFTETGKTGCHNISPCSGCI
                     YLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKS
                     KVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTCSSW"
     gene            1804..>1899
                     /locus_tag="ACHJZ2_005172"
     CDS             1804..>1899
                     /locus_tag="ACHJZ2_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HFO4571065.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKH"
BASE COUNT          658 a          299 c          361 g          581 t
ORIGIN      
        1 acataattat tgatagtgag tacaagtcaa ataaggcagt aaaggaatat gtagaaattt
       61 taaaagaatt caatatatgc ccagaaaagg tagcagaata cctaagcgag ttcagcggaa
      121 aacatttcct cgatgtatat aattattatt ccaattgaaa aaaatataaa gtatctatca
      181 cggcactctc agttgagtat cacaggcaca tgtattttag ccccctgagt ctacagacaa
      241 ccaatattac ttaaataagt gataatctta atgctgtttt ttagaacaga tgattgatat
      301 ttttgggccg gagaaacgca gacaggcttt gttgaataaa tcgagctttt gctgagttta
      361 aggatcagat cactcatttt cctgcaaaag ctcattgggc gaaacaatct gactcagaga
      421 acccggatta aacgtctggc ccgcagaatc atctatttct cacgcactgt agtacgtaat
      481 aaaaaagtca tcggtacttt tatcaaaaaa catatattct attaattatt gccatcacct
      541 ttttcttatt gacaacatta ctctgctgat tgttaaaatc taacctgtat tcagggttac
      601 gattctatac aatagaacag ggaggggaaa ggatgtcatc gttaatgaga ctttttgtta
      661 atgtcaacaa tgctatgaat ttattttaga aaaacaatta agctgacgta gtgttccgtc
      721 taaatgacgg ggcgatatta acatgattag aacaagagga attttatgtt atcttcatta
      781 aatgtccttc aatccagctt cagaggaaag acagctttat caaatagtac acttctccag
      841 aaagtttctt ttgctggaaa agaatatcct ctggaaccta ttgatgaaaa aacccctatt
      901 ctttttcagt ggtttgaagc aaggccagag cgatacgaaa aaggagaagt accaatattg
      961 aataccaaag aacatccgta tttgagcaat attataaatg ctgcaaaaat agaaaatgag
     1021 cgtataatcg gtgtgctggt agatggaaat tttacttatg aacaaaaaaa ggaatttctc
     1081 agtcttgaaa atgaatatca aaatataaaa ataatctacc gagcagatgt ggatttcagc
     1141 atgtatgata aaaaactatc tgatatttac cttgaaaata tccataaaca agaatcatac
     1201 cctgccagtg agagggataa ttatctgtta ggcttattaa gagaagagtt aaaaaatatc
     1261 ccagaaggta aggactcttt gattgagtca tatgcagaaa aaagagaaca tacttggttt
     1321 gattttttca ggaatttggc catgttgaag gctggaagtt tgtttacaga gacgggaaaa
     1381 actggatgcc ataacatatc gccctgtagc ggatgtatat atcttgatgc cgacatgatt
     1441 attaccgata aattaggagt cctgtatgct cctgatggta tcgctgtgca tgtagattgt
     1501 aatgatgaga taaaaagtct tgaaaatggt gcgatagttg tcaatcgtag taatcatcca
     1561 gcattacttg caggcctcga tattatgaag agtaaagttg acgctcatcc atattatgat
     1621 ggtctaggaa agggtatcaa gcggcatttt aactattcat cgttacacga ttataatgct
     1681 ttttgtgatt ttattgaatt taagcatgaa aatattatac cgaataccag tatgtatacc
     1741 tgcagttcat ggtaagtctt ccggttgtgg ttgaaaaata tcgttcttta ggtatagatt
     1801 cagatgatta atcctgttac taatactcag ggcgtgtccc ctataaatac taaatatgct
     1861 gaacatgtgg tgaaaaatat ttacccggaa attaaacat
//
LOCUS       DAZFBR010000295         1898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFBR010000295 DAZFBR010000000
VERSION     DAZFBR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.298"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            201..704
                     /locus_tag="ACHJZ2_005173"
     CDS             201..704
                     /locus_tag="ACHJZ2_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO4571066.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(784..1035)
                     /gene="yecJ"
                     /locus_tag="ACHJZ2_005174"
     CDS             complement(784..1035)
                     /gene="yecJ"
                     /locus_tag="ACHJZ2_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO4571067.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1150..1236)
                     /gene="azuC"
                     /locus_tag="ACHJZ2_005175"
     CDS             complement(1150..1236)
                     /gene="azuC"
                     /locus_tag="ACHJZ2_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO4571068.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1498..1821
                     /gene="yecR"
                     /locus_tag="ACHJZ2_005176"
     CDS             1498..1821
                     /gene="yecR"
                     /locus_tag="ACHJZ2_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO4571069.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGISDRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          502 a          441 c          408 g          547 t
ORIGIN      
        1 tgaaatgtaa aaatatataa acttgatgat ttaagcattt tcttataccc gttcagacgt
       61 tattcttatt tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa
      121 aggaattatt ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc
      181 tggatataag gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa
      241 tgaaccgcga gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac
      301 agagtctgaa cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa
      361 tgatgcgcat gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg
      421 atgttcccgg tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat
      481 acgagcaacg ttctagtacg ttggcacagt tagccgatga agcgaaagaa ctgaatgatg
      541 attcaaccgt caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggcctgttgc
      601 tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc ccagtgcaga
      661 ccgaccaaca tgttctgaat gtcgtgtcgc atcagctgca ttgatcatac atcgacgcta
      721 atgcattgca ccgatgaagt ttttgagaaa ccgctgcctc atcttttttg aagcagcggt
      781 tttttaatgg gattcgcctt gtggggtaaa cttgagctca atcagcgcga tggctttttg
      841 gattgcccgc atggtgaccg ggtctgcggc agcgggatga tgagtaaagt cgatattctt
      901 cagctgactg gacatttttt cacgaacttc aacgggcgcg attacatcga gaacatccag
      961 aatttgtttg ataaccaact ggcaagcaac cacatcagaa accagttcct gatcggcatt
     1021 cagcgtctgg gacatcgtaa actcctgata gcattttgaa agccgttata gtagcggctt
     1081 cacatcttca acgatagtca catccaccct catcaggaca caaaaaaacc tgccggagca
     1141 ggttttttgt tatcggaaca tattgcctgg cggtacgtct ttgaacgtct tgcaatagtt
     1201 attgaacata cttttcagga ttttgcgcag tttcatcgcg gcactccgac catttgttat
     1261 acaggtgtta ttgtctttgc gcttataata tgacaaccat cacaaaaatc aatctttatg
     1321 tgatacaaat cacataaata cccctttaat gttataaaaa tgataatcaa agaacagccc
     1381 cctatttctg acacctacag atggcaagaa atagcgcctg ccaggcgtct tttccggcca
     1441 ttgtcgcagc gctgtaacgc gtaaaatagt gctttctctt actcttctgg ctggaccatg
     1501 agacttctga ttctgactct ttcattaata acgctggcgg ggtgtacggt tactcgccag
     1561 gcccatgtga gcgaagttga cgcggcaaca ggtattgtac ggttggttta tgaccaggct
     1621 tttttgcagc atgcccatac cgatcgttat gtcagtcgcg ggatctccga tcgcgcatgc
     1681 caacaggaag gctataccca cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt
     1741 tttgcgggtt ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc
     1801 gccttccctg tctttctcta aatattttaa tcagcgaggg gatcttcgct gattaaagaa
     1861 atagcggtaa tgcgttttat tcccattcgt atttttaa
//
LOCUS       DAZFBR010000296         1835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFBR010000296 DAZFBR010000000
VERSION     DAZFBR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.299"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1654
                     /locus_tag="ACHJZ2_005177"
     rRNA            <1..1654
                     /locus_tag="ACHJZ2_005177"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1749..>1835
                     /gene="rrf"
                     /locus_tag="ACHJZ2_005178"
     rRNA            1749..>1835
                     /gene="rrf"
                     /locus_tag="ACHJZ2_005178"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          464 a          412 c          575 g          384 t
ORIGIN      
        1 cgaatgctga cataagtaac gataaagcgg gtgaaaagcc cgctcgccgg aagaccaagg
       61 gttcctgtcc aacgttaatc ggggcagggt gagtcgaccc ctaaggcgag gccgaaaggc
      121 gtagtcgatg ggaaacaggt taatattcct gtacttggtg ttactgcgaa ggggggacgg
      181 agaaggctat gttggccggg cgacggttgt cccggtttaa gcgtgtaggc tggttttcca
      241 ggcaaatccg gaaaatcaag gctgaggcgt gatgacgagg cactacggtg ctgaagcaac
      301 aaatgccctg cttccaggaa aagcctctaa gcatcaggta acatcaaatc gtaccccaaa
      361 ccgacacagg tggtcaggta gagaatacca aggcgcttga gagaactcgg gtgaaggaac
      421 taggcaaaat ggtgccgtaa cttcgggaga aggcacgctg atatgtaggt gaagtccctc
      481 gcggatggag ctgaaatcag tcgaagatac cagctggctg caactgttta ttaaaaacac
      541 agcactgtgc aaacacgaaa gtggacgtat acggtgtgac gcctgcccgg tgccggaagg
      601 ttaattgatg gggtcagcgc aagcgaagct cttgatcgaa gccccggtaa acggcggccg
      661 taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cctgcacgaa
      721 tggcgtaatg atggccaggc tgtctccacc cgagactcag tgaaattgaa ctcgctgtga
      781 agatgcagtg tacccgcggc aagacggaaa gaccccgtga acctttacta tagcttgaca
      841 ctgaacattg agccttgatg tgtaggatag gtgggaggct ttgaagtgtg gacgccagtc
      901 tgcatggagc cgaccttgaa ataccaccct ttaatgtttg atgttctaac gtggacccgt
      961 gatccgggtt gcggacagtg tctggtgggt agtttgactg gggcggtctc ctcctaaaga
     1021 gtaacggagg agcacgaagg ttggctaatc ctggtcggac atcaggaggt tagtgcaatg
     1081 gcataagcca gcttgactgc gagcgtgacg gcgcgagcag gtgcgaaagc aggtcatagt
     1141 gatccggtgg ttctgaatgg aagggccatc gctcaacgga taaaaggtac tccggggata
     1201 acaggctgat accgcccaag agttcatatc gacggcggtg tttggcacct cgatgtcggc
     1261 tcatcacatc ctggggctga agtaggtccc aagggtatgg ctgttcgcca tttaaagtgg
     1321 tacgcgagct gggtttagaa cgtcgtgaga cagttcggtc cctatctgcc gtgggcgctg
     1381 gagaactgag gggggctgct cctagtacga gaggaccgga gtggacgcat cactggtgtt
     1441 cgggttgtca tgccaatggc actgcccggt agctaaatgc ggaagagata agtgctgaaa
     1501 gcatctaagc acgaaacttg ccccgagatg agttctccct gaccctttaa gggtcctgaa
     1561 ggaacgttga agacgacgac gttgataggc cgggtgtgta agcgcagcga tgcgttgagc
     1621 taaccggtac taatgaaccg tgaggcttaa ccttacaacg ccgaaggtgt tttggcggat
     1681 gagagaagat tttcggcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     1741 agaatttgcc tggcggcctt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     1801 tgaaacgccg tagcgccgat ggtagtgtgg ggtct
//
LOCUS       DAZFBR010000297         1832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFBR010000297 DAZFBR010000000
VERSION     DAZFBR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.300"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..98)
                     /locus_tag="ACHJZ2_005179"
     CDS             complement(<1..98)
                     /locus_tag="ACHJZ2_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571070.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSD"
     gene            complement(82..>1832)
                     /locus_tag="ACHJZ2_005180"
     CDS             complement(82..>1832)
                     /locus_tag="ACHJZ2_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4571071.1"
                     /translation="HRETARSFGGAGSTAGYEQVTAYTLTGQLQSRHLNLPQLDCDYD
                     WNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELH
                     PDSTLTAWPDNRIAEDAHYVYRYDEYGRLTEKTDRIPEGVIRMHDERTHHYHYDNQHR
                     LVFYTRIQYGEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTVQTGTTRIQTVYRPGSFTPLIRIETENGEREKAQRRSLAEKLQQEGSEDGHGVV
                     FPAELVRMLDRLEEEIRADRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARTLHLYHC
                     DHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQHDEESGLYYNR
                     HRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSG
                     VPGLIVANSITNSAYQFGYDMDAIVGGAHNGAADAMRYCYLMCRMTKTFGSTIADVIG
                     KNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNIGQLFGLDGIKA
                     DNPIKAKQGSSDASNY"
BASE COUNT          349 a          566 c          448 g          469 t
ORIGIN      
        1 atgtcagaat ttgaattgtt atcaaaccca aggtaaacaa caaataaaca aagggtaatt
       61 aaaaaaatga caagtttgcc tctaataatt tgaagcatct gaactccctt gctttgcttt
      121 tattggatta tctgctttta taccatctaa cccgaagagt tgcccaatgt tatatttttc
      181 aatacatgca tcgctacatt tggcagaaaa atcgccacaa gcaataccga cagtgttatt
      241 tttaagatcc atgattcttt ctttagctgg ctgaccttgt ctatccccag ccgcctcatg
      301 atttttacct atcacgtcag ctattgttga tccaaatgtc ttagtcattc gacacatcaa
      361 gtaacaatat ctcattgcat cggcggcccc attatgagct ccgccaacaa tagcatccat
      421 atcataaccg aactggtaag cactgttagt aatgctgttt gcaacaatca atccaggtac
      481 accagatacg gcataaatta gtccacctga tgtcatattc tcaatcgcat ctaaccccat
      541 tgggtctatc acttgtatgg gattcaacgg atactgatac atattccatc cacctctcaa
      601 cccaatcggg tccggggtga tataccgccc ctgcaacgga tcgtagtacc ggtgacggtt
      661 atagtacagc cccgactcct catcatgctg ctggcccggc agacggtacg gctggtgcag
      721 gtgatgcggg ttctcctcat taagctggtt gccccattca tcatattccg cgctccacgc
      781 cgtattgccg tcttcgctga taagcgccag cggcagtccc cggtggtcac agtggtacag
      841 atgcagcgtg cgcgccggcg tgtattccgg ctccacctgt ttttccagtt gctccaccgt
      901 cagtccgcac tgcgcaagcc acgcccggct ttcactgctc acgcggtctg cccggatttc
      961 ttcctccagc ctgtccagca tcctcaccag ttctgccggg aacaccacac cgtgaccgtc
     1021 ctcactccct tcctgctgga gtttctccgc caggctgcgg cgctgcgctt tctcccgctc
     1081 gccgttctcc gtttcgatgc ggatgagcgg tgtgaagctc cccggccggt ataccgtctg
     1141 gatacgtgtg gtgccggtct gtaccgtcgt cagcctgtcg ccgtcccacc cgtaccaggt
     1201 cacctccggt ttacgcgaca gcgacatcca ccccgtcagg tcacgctccc gccgccacac
     1261 ccgtttcccc gtccggcggc ccagcgggtc atagaggtag cggctctcga ccagcggctc
     1321 gccgtattgt atccgcgtgt agaacaccag gcggtgctgg ttgtcgtaat ggtagtggtg
     1381 ggtccgctcg tcgtgcatcc ggataacccc ttccggaatg cggtccgtct tctccgtcag
     1441 cctgccgtat tcatcgtagc ggtagacata gtgcgcatct tccgcgatgc ggttatccgg
     1501 ccacgccgtg agcgtgctgt ccggatgcag ttccgggtcc ggcagccggt ttcctgccgg
     1561 gtccgttgca tacgggatat cgatatccag gttcgctgcc gtggtgtgga cgcccgtcag
     1621 ccttcccgtg tcgctgtaac ggtactcccg gctctcctgc gggccgctga tgcggattag
     1681 ctgtccgttg tcgttccagt cgtagtcaca gtcaagctgc gggaggttca ggtgcctgct
     1741 ctgtagctgc cctgtgaggg tgtacgccgt gacctgttcg taccccgctg tactgcccgc
     1801 cccgccgaag ctgcgggccg tctcacggtg ca
//
LOCUS       DAZFBR010000298         1826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFBR010000298 DAZFBR010000000
VERSION     DAZFBR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.301"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            280..587
                     /locus_tag="ACHJZ2_005181"
                     /pseudo
     CDS             280..587
                     /locus_tag="ACHJZ2_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(836..1291)
                     /gene="espF"
                     /locus_tag="ACHJZ2_005182"
                     /pseudo
     CDS             complement(836..1291)
                     /gene="espF"
                     /locus_tag="ACHJZ2_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
     gene            complement(1376..1654)
                     /gene="escG"
                     /locus_tag="ACHJZ2_005183"
     CDS             complement(1376..1654)
                     /gene="escG"
                     /locus_tag="ACHJZ2_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle protein
                     cochaperone EscG"
                     /protein_id="HFO4571072.1"
                     /translation="MVNDISANKILVWAAVAAANHKLPKYAEAILNVFPQIIPDKKDI
                     AHLEFIILFGLNRKNDAVKALEDCMDDETSQLLYSLVHENGSGWVRGF"
     gene            complement(1660..>1826)
                     /gene="escF"
                     /locus_tag="ACHJZ2_005184"
     CDS             complement(1660..>1826)
                     /gene="escF"
                     /locus_tag="ACHJZ2_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO4571073.1"
                     /translation="SVPELLNSTDLVNDPEKMLELQFAVQQYSAYVNVESGMLKTIKD
                     LVSTISNRSF"
BASE COUNT          556 a          415 c          349 g          506 t
ORIGIN      
        1 aacaaaaccc cttttaatcc cttatatatc aggctttctt tgtgtacagg ggtcaaccga
       61 ctcaacctgt atcaagttgc ttttgaggat actttagggg gtattatttc ctcttgaaat
      121 attagcaccc cataatggga aaaaggtaat acataacaat ggtttttaac agtatctttg
      181 ttatacaagg gggcattgag gatatcagga aaaatctcaa aatcggtacg gacaggaaac
      241 tacgtgggca gcaaaaacgt atggtaacac cgggaaatgc aacgcccacc agtcgggcta
      301 atccaccact ccaatcgtgg ttcgcagtac tgcacacacg attaccgggc tatacaggag
      361 cggtttggtc tgaaaacatc catgccgcgt aaaggtaacc gttacgacaa cacctcaatg
      421 aaaaacttat agtaaacgct gaagaatgaa actatcgttt taataaccgg gatgaagcca
      481 tctaagtaat acggaaatac attgagattt tctacaacca tcagcgccgc cactcccgtc
      541 tggggaatat ctccctggta gccttcagga aaaatataac cagataactg cgtaaaaaaa
      601 gaacaaatgg tggtgtccgc tattgccagt atacctgagt ttactgcctt tcagattatc
      661 acaatcaata gcgaacagac ttttaagctc tgtgcgcttt taacaaaacg tccctttctc
      721 cactttcacc gtataataat acattattta ttacccctcc aataaaaacc gatattacta
      781 atcacattta ttaccacaca atttgatatc ggtttttatt agttggttac cctttcttcg
      841 attgctcata ggcagctaaa tgatctttta atgcctgtgc aaggggcggt agtccactag
      901 gccctgtcgg tggcggtggt gcctgacggg cgggcttaaa acctaaagcc tcaggacctt
      961 tcgatttttc ataggcagcc aagtgctctt ttaatgcctg tgcaatgggc ggtaaaggtc
     1021 gggatgcccc ggatgcctgt ccacttgtcg gtggcggcgg tgccggacga gagggagtaa
     1081 atgaagtcac ctggctgctc acattaaaaa tcgttctcgc attaacattc gacgagcctg
     1141 gagaaaaagg ggaatgaact ttcaccggag taagacgcac ggcctgaggg gctacagaaa
     1201 atccagttcc ccccgcagag ctcactcgac ttgcgatacc tacaagctgc cgccctagtg
     1261 tagaagcagc gttactaatt ccattaagca taatttatgc ctctttatat ctaagcttgg
     1321 ctaactctca tatatatcaa aacgaataat aaaatgattt ttaaatcaat ataaattaaa
     1381 atcctcgtac ccagccacta ccattctcat ggaccaaact gtacaataac tgactcgtct
     1441 catcatccat acagtcctcc aaagccttta ccgcatcatt ttttctattt aatccaaata
     1501 agataataaa ttctaaatgt gcgatatctt ttttatcagg tataatttgt gggaatacat
     1561 tcaggatagc ttccgcatat ttgggaagct tatggtttgc cgcagctaca gccgcccaca
     1621 ccagtatctt attagcagaa atatcattaa ccattaaaat taaaaactac ggttagaaat
     1681 ggttgagacc agatctttta tcgttttcaa cattccactt tctacgttaa cataagcaga
     1741 atattgctga accgcaaact gcaactctaa cattttttca gggtcattaa ccaaatcggt
     1801 gctattaagt aactctggca cagaat
//
LOCUS       DAZFBR010000299         1808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFBR010000299 DAZFBR010000000
VERSION     DAZFBR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.302"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(28..387)
                     /locus_tag="ACHJZ2_005185"
     CDS             complement(28..387)
                     /locus_tag="ACHJZ2_005185"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO4571074.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
     gene            1073..1363
                     /locus_tag="ACHJZ2_005186"
     CDS             1073..1363
                     /locus_tag="ACHJZ2_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4571075.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            1387..1638
                     /locus_tag="ACHJZ2_005187"
     CDS             1387..1638
                     /locus_tag="ACHJZ2_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4571076.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1686..>1808)
                     /gene="traT"
                     /locus_tag="ACHJZ2_005188"
     CDS             complement(1686..>1808)
                     /gene="traT"
                     /locus_tag="ACHJZ2_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO4571077.1"
                     /translation="GNQHKYQTRVVSSANQVNLKFEEAKPHLEDQLAKSIANIL"
BASE COUNT          523 a          346 c          388 g          551 t
ORIGIN      
        1 aactgtgaat aatttagatg ttgggcgtta tgaaagtaat aaaactcccc atataatgat
       61 agcaactaaa atcgtaatca acactgcggc tgaacccata tcttttgctc tgcctgataa
      121 ttcgtgatgt tctgaaccaa ttctgtcaac aactgcttca attgcactgt taattatttc
      181 aacaataatt accatcattg ccgaaccaat gagtaatact cgagtaacag agtcaacatc
      241 cagccaaaag gatattatca gtgctgacag aaccagaatg cactcctgac gaaaggctgc
      301 ttcatagcgc catgccgcac ataagccttt ccatgaataa cccgctgctt taataattct
      361 ggtaacacca gtggtcgtat tagccataaa tacctcttat ttaattatat gtctgaatat
      421 gcattataaa atatcagtta acattaacag gattttgtaa taagccacat ttattctttc
      481 gggaattaaa aaacataacg aaattttgcc tcattctgca tttcattgaa atagtatgtt
      541 tctcatcgaa agcaagtttg tcaatataaa tttgtaacag aaatgacttt ccggggataa
      601 acaaaactgt tcatggcaat ggctttctct gtcgccaaca tggatacatt gttccggata
      661 aagcactgat gtcattgact gacgcagact atcaggccat ctgatgcttt cttttccttc
      721 accggtattg atgatatgcc ttatgaaggg ggacttattc ctgtaaccca atcacaggca
      781 ctgtgattac tggtttatga ttgatgctga tttgagcaga tatgcacagc agttgtggat
      841 tcaggggata ttttctgcca gatattcata ccagattgat gacctctttg ctcagatgct
      901 cttcgttctg agccagccag gattttgtgg ccacatacat tgttcgccga tggggaatcg
      961 agacaggcga ttggctgtga tgcgattttg ccgctaactg aggatgagtc cgcccctccc
     1021 ggggaattcc tgtaagttaa gccgtacctg ttgattagca ctaaactggg taatgaattt
     1081 atctgccagt atgaatgatg ccggggacgg ttttgaactc ctgatgtcgg gtaatatgca
     1141 ggattttaat atttgttgtc gtcgggataa tcattatcct gaacataaac tgataccttt
     1201 atcagagtta tttgacgtta attatggact gaatcttgag ctgaataaac tggagaaaga
     1261 ttcttcagga attaattttg tttcaagaac gtccaaaaat aatggcgtct ccgccagggt
     1321 taaattaaaa gatggtatta gtccatgcca gcagctgtgt taactgtagc agcaggaggt
     1381 tctgttatgg aatcgtttgt acaggattca ccattttata gtggaagaga tctttattgg
     1441 ttacgtccta aagttgaact gactctggaa gagaaattgt actactgctc ttgtataaga
     1501 agaaacagac acaaatacag ctacgggcgt caggcaaacc gaacgctgaa gaatttactg
     1561 gtaccgtcat tagattctgt tccggcatgg gtatatggtg tgacagggaa aataatatct
     1621 gaattgtctg aacgttgaga ctaagagggc cggaggccag gtgaatggcc accgggatat
     1681 ttatatcaga gtatattggc gattgatttt gccagctggt cttccagatg aggtttagct
     1741 tcttcaaact tcagattaac ctggttggca ctggatacaa cccgggtctg gtatttatgc
     1801 tggttgcc
//
LOCUS       DAZFBR010000300         1808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFBR010000300 DAZFBR010000000
VERSION     DAZFBR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.303"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..466
                     /locus_tag="ACHJZ2_005189"
     CDS             <1..466
                     /locus_tag="ACHJZ2_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4571078.1"
                     /translation="SALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNK
                     LPDLRGEFIRGWDDGRGIDAGRVLLSIQTGMLEKHRHIVVANDGYDTKDEWELATIFK
                     KTYTQGRGLDATNTGGSLIPSPTLHSRGSIGNTGGSETRPRNIAFNYIVRAA"
     gene            466..1068
                     /locus_tag="ACHJZ2_005190"
     CDS             466..1068
                     /locus_tag="ACHJZ2_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4571079.1"
                     /translation="MDNAVLNSELIATKAGNITVYNYDGKTREYISTSNEYLAIGVGI
                     PAYSCLDEPGIHKAGYAICRSMDLNSWEYVPDHRGEIVYNTETGDAKEITAPGDYPEN
                     TTTIAPLTPFDKWDGEKWVTDTEAQHGAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKPTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(1040..1483)
                     /locus_tag="ACHJZ2_005191"
     CDS             complement(1040..1483)
                     /locus_tag="ACHJZ2_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4571080.1"
                     /translation="MNEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVNQNIISEFM
                     AEPPQGKIRIAGDDGLPAWADIPPPTHEELIEITESERQLLINQANEYMNSKQWPGKA
                     AIGRLKGEELAQYNLWLDYLDALELVDTSSAPDIEWPTPPAVQAR"
     gene            complement(1504..>1808)
                     /locus_tag="ACHJZ2_005192"
     CDS             complement(1504..>1808)
                     /locus_tag="ACHJZ2_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4571081.1"
                     /translation="ASGDGWMKLPNGKILQYGRGAVTPTLSTQTMRITFSIPFSKKAD
                     CAMLTHSGDGGAPLGAGRGFVMTAEGPTLTGFNSAYRTSSTSDTVSMNYSWWAVGE"
BASE COUNT          496 a          431 c          420 g          461 t
ORIGIN      
        1 ctctgcatta cctgttggtg tgcctgttcc atggccttca gccacaccgc caacaggctg
       61 gctgaaatgc aacggtgcgg ctttttctgc tgaagaatac ccggaactgg caaaggctta
      121 tccgacaaat aaattgcctg atttacgtgg tgagtttatt cgtggctggg atgacgggcg
      181 cggtattgat gcaggacgtg ttttattgag cattcagaca gggatgctgg aaaaacaccg
      241 ccatattgtt gttgccaatg atggttacga cacaaaagat gaatgggaac tggctacgat
      301 tttcaaaaag acatacacac aaggacgggg acttgatgcc acaaatacag gagggagtct
      361 gatcccatca ccgacacttc attcacgagg gagtatcggt aatactggcg ggagtgaaac
      421 ccgtccacga aatattgcat ttaactatat cgtgagggct gcataatgga taacgccgta
      481 ttaaatagcg agcttattgc cacgaaggcg gggaatatta ccgtctataa ctatgatggt
      541 aaaactcggg aatatatttc tacttcaaat gaatatcttg ccattggtgt cggtatccct
      601 gcatattcct gtctggatga acctggtata cataaggcgg gttatgctat ctgccgttcg
      661 atggatttaa actcatggga atatgtgcca gaccatcgcg gtgaaatcgt ctataacacc
      721 gaaacgggag acgccaaaga aatcacagct ccgggtgatt atcctgaaaa tacaaccact
      781 atcgccccgt taacgccatt cgataaatgg gatggtgaaa aatgggtgac agatactgag
      841 gcacaacacg gtgccgcagt agaagcggca gaagcacagc gccagtcact gattgatgca
      901 gcaatggctt ccatcagtct gattcaactg aaattgcagg ccggacgtaa accgacgcag
      961 gcagaaacaa ccagacttaa cgctgtgctg gattacattg acgcggtgac ggcaacagat
     1021 accagcaccg cgccggatgt catctggcct gaactgccgg aggcgtaggc cattcaatat
     1081 ctggcgcact ggaggtatca accagctcca gtgcatccag ataatccagc cacaaattat
     1141 attgcgccag ttcctcacct ttcagacgac caatagcggc tttaccgggc cattgtttac
     1201 tgttcatgta ttcgttggcc tggttaatta gtagctgtct ttctgattca gtaatttcaa
     1261 taagttcttc atgcgtgggt ggaggaatat ctgcccacgc aggcagccca tcatctccgg
     1321 caatacggat ttttccttgt ggcggttcag ccataaactc actgataata ttttgattta
     1381 cttccttagc gtctgataaa tcccatccct ctgatttata tttatcaatc atatccacag
     1441 ggaaaaaagc attatgcctt gcgctataaa catattcatt catataaatc accctgaata
     1501 aaattactca ccaacagccc accaactgta attcatcgat accgtgtcgc tggttgatga
     1561 cgttctgtaa gcagaattaa agccggttaa cgttgggcct tctgcagtca tcacgaaccc
     1621 tcgcccagcg cctaaaggcg caccgccatc accagaatga gtaagcatgg cgcagtccgc
     1681 ttttttggag aaagggatgc tgaatgtaat tctcattgtt tgcgtcgata atgtcggcgt
     1741 aaccgcacca cgaccatatt gcaggatttt cccgttgggt aatttcatcc atccatcacc
     1801 actggcaa
//
LOCUS       DAZFBR010000301         1767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFBR010000301 DAZFBR010000000
VERSION     DAZFBR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.304"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..66
                     /locus_tag="ACHJZ2_005193"
     CDS             <1..66
                     /locus_tag="ACHJZ2_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571082.1"
                     /translation="VRGQLVPGERLGIKITELECA"
     gene            63..752
                     /locus_tag="ACHJZ2_005194"
     CDS             63..752
                     /locus_tag="ACHJZ2_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4571083.1"
                     /translation="MNNHYLQFVRELLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYKELALLVGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            963..1038
                     /locus_tag="ACHJZ2_005195"
     tRNA            963..1038
                     /locus_tag="ACHJZ2_005195"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:996..998,aa:Ile,seq:cat)
     gene            1046..1122
                     /locus_tag="ACHJZ2_005196"
     tRNA            1046..1122
                     /locus_tag="ACHJZ2_005196"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1080..1082,aa:Arg,seq:tcg)
     gene            1136..1212
                     /locus_tag="ACHJZ2_005197"
     tRNA            1136..1212
                     /locus_tag="ACHJZ2_005197"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Arg,seq:tct)
     gene            1386..1767
                     /locus_tag="ACHJZ2_005198"
                     /pseudo
     CDS             1386..1767
                     /locus_tag="ACHJZ2_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          467 a          383 c          491 g          426 t
ORIGIN      
        1 gtgcgcggtc agctcgttcc tggtgagcgg ctggggataa aaatcacaga actggagtgc
       61 gcatgaataa ccactattta cagtttgtgc gtgagctgct cattatcgct accgccgatt
      121 tgagtggggc aacaaaaggt cagcttgaag cctggcagga gaatgccatg ttcgatacag
      181 ggcgttacag gcgaaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      241 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggtgacgtca attcctctgg
      301 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagaac
      361 atcataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
      421 cgataacaca gtgggcgtgg aatgaattta atactcaatc cggtaccaga aaaattgcag
      481 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
      541 aactttttgg tggggaaggt tatgaataca aggagctggc attactggtg ggagtgacaa
      601 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
      661 gactggatag tgaggcttta ttgtttgtaa tgagaacgcg ttcaaaacaa aaggcggcat
      721 tttcaaagca aagtgttgca aaagtagatt gaaaggcata tatttcatgc aaatctgata
      781 ttttgccgat tttgtacgtg atggcaaaag caaacaaaac ccgcccacaa gcgggttttt
      841 ttgtgccact tatctcggat agacatggtg aatgcgctgg tggaggaagc aagggtaatt
      901 tttaaccagg tgattcttga atgcttgcaa cattgatttc gtaacgttat tatcctgcgc
      961 ccggcccttt agctcagtgg tgagagcgag cgactcataa tcgccaggcc gctggttcaa
     1021 atccagcaag ggccaccatc acataccgcc attagctcat cgggatagaa cgccagcctt
     1081 cgaagctggt ttcgcggggt tcgagtctcc gatggcggtc cattatcggt attctgcgtt
     1141 tttagctcag ccggacagag caattgcctt ctaagcaatc ggtcactggt tcgaacccag
     1201 tacaacgcac cacacttatt ttccctcgct cgcttttgcg gacctttttt gtatccgcac
     1261 cacgcccggc gcataccaac cacagagcct ttcgggggga gcttatggag tggtcagtgt
     1321 gactttctct gtgggcagat cgctcccggg cgttggctca cccacccaaa ggaacgtcac
     1381 gatgtttggt atttttggta aaaaaagccc gcagagcggc aacggaaatt aaaaagtttg
     1441 aaaaacgcga tctggcacag gtggtgatta acgccgcata cctggtggcc tgtgcagatg
     1501 gtgaatgtga ggcttcctag aaagcgaaga tcgaacaggt actgcgtaat cagcctgcgc
     1561 tgtacgcgtt tacgtcagaa attaatgcga ttagcgcaac cattatcggt cagctggata
     1621 cgaactttaa aactggtcgt cgtgcggcgt tacgtgagat cgaggatgtg aaacacgata
     1681 cgcgtgaagc ggaagatgtg ctggatgtgg cggtggccat tgcggaggca gacggcgaaa
     1741 ttgagccgga agagcgcaag gtgctgg
//
LOCUS       DAZFBR010000302         1762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFBR010000302 DAZFBR010000000
VERSION     DAZFBR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.305"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            3..215
                     /locus_tag="ACHJZ2_005199"
     CDS             3..215
                     /locus_tag="ACHJZ2_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4571084.1"
                     /translation="MIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDERRR
                     LAVQGCRTCASCQEDLELISKQRGSK"
     gene            212..1159
                     /locus_tag="ACHJZ2_005200"
     CDS             212..1159
                     /locus_tag="ACHJZ2_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4571085.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EEIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIAVDADYDHD
                     RIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            1161..1337
                     /locus_tag="ACHJZ2_005201"
     CDS             1161..1337
                     /locus_tag="ACHJZ2_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571086.1"
                     /translation="MALTHRELCQIAYKFLKRNGFKVCFHDRFIAVTSTGEQPDAMGF
                     RNSASCLIGEMFSC"
     gene            1671..>1762
                     /locus_tag="ACHJZ2_005202"
     CDS             1671..>1762
                     /locus_tag="ACHJZ2_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valyl-tRNA synthetase"
                     /protein_id="HFO4571087.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRA"
BASE COUNT          539 a          332 c          461 g          430 t
ORIGIN      
        1 acatcattga ttcagcatca gaaattgaag aattacagcg caatacagca ataaaaatgc
       61 gtcgcctgaa ctaccagact gtatccgcaa ctcattgttg tgagtgtggc gatccgatag
      121 atgaacgaag acgcctggca gttcagggtt gtcggacttg tgcaagttgc caggaggatc
      181 tggagcttat cagtaaacag agaggttcga agtgagcgaa attaattatc aggcactgcg
      241 tgaaaaggca gagaaagcaa ctaaaggaag ctacatcgta gggcatacat ctgttaacca
      301 gcacggcaat ttaacaggag tttttgtttg ccaaaaatgg aaaggagaac ccggtggtgt
      361 gattgcggaa tgtcatgtta actgcctggt tgaaacagat gttcaggctt atgcaaacgc
      421 tgaatttatt gctgccttta atccaaatgt tgcgctggcg cttctggatg aacgggaaag
      481 aaaccagcaa tacatcaaac gccgcgacca ggagaacgag gagattgcgc ttacggttgg
      541 gaagctgcgt gttgagcttg aagcagcaaa atcaaaactc aacgagcagc gtgaatatta
      601 cgagggagta atcgcggatg gaagtaagcg catagcagaa ctggaaaaac aatgcgccga
      661 atgggagcga aaagcattaa gcaactttga agagtgtgct gcgatggctg aacgtatcga
      721 agagatgcag acaaaatctg caccagattc gtttggcatc atcggtgaaa atattcgaac
      781 acaggacaat cgaataacgt cagatcccat gttttgtgtg tatcaaaagc gcgaaatcgc
      841 tgttgatgct gattatgacc atgaccggat tgtctgggtt gacgaagatg gcaatgaagc
      901 caataaacgc catagtcgtc gtctcgagct acttcatgaa aactttcgag agccaccaga
      961 aaaatggcgg cgcgttgctg tgaaagatat tgatgaattc gttacctgct gtttcaccga
     1021 acagggttgt aaagactacc tggcagtcaa tggtcacaat cttcgcttgc catttatata
     1081 tgtaaaaagc ggtttcagga acgctgaata tatcggcata agaaactggc ttgctggcat
     1141 tcgcatcaaa ggagagtgat atggcgttaa cacaccgcga actctgtcag attgcgtaca
     1201 agttccttaa gcgcaacggt ttcaaggttt gttttcatga ccgctttata gctgtaacca
     1261 gtaccggaga acagccagat gctatgggat tcagaaattc agcatcatgc ctgattggcg
     1321 aaatgttctc gtgctgactt gttggcagat agaaaaaagc gttttcgtaa aaatccgtct
     1381 cttggaatgg gcgactggcg attctttatt agtgagccgg gaattatttc aattgaggat
     1441 ttaccacctg gctggggatt acttcacgtt gttaacggaa gagtacggaa agtacatggg
     1501 tggcccaagg gtaattgctg ttggggtaat cctgacgata agccatttac tggaaataag
     1561 caggttgaat gcgattacat gttgtctgca ttaaggcgca tggagttgag agggcacctt
     1621 aatgaaatat atgacggtgt aattgttaat aagaaagaag gaaacgcggc atgaccacta
     1681 ttaccgacaa agaactgatt aaagaaatca aagagcgcat aggcagcttg gacgttcgag
     1741 acaatattga gcgccgtgct ta
//
LOCUS       DAZFBR010000303         1734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFBR010000303 DAZFBR010000000
VERSION     DAZFBR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.306"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..207
                     /locus_tag="ACHJZ2_005203"
     CDS             <1..207
                     /locus_tag="ACHJZ2_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein O"
                     /protein_id="HFO4571088.1"
                     /translation="GRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQ
                     QAGMIASKPKLDLTNTDWIYGVDL"
     gene            204..905
                     /locus_tag="ACHJZ2_005204"
     CDS             204..905
                     /locus_tag="ACHJZ2_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4571089.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            902..1204
                     /locus_tag="ACHJZ2_005205"
     CDS             902..1204
                     /locus_tag="ACHJZ2_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO4571090.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            1272..1604
                     /gene="emrE"
                     /locus_tag="ACHJZ2_005206"
     CDS             1272..1604
                     /gene="emrE"
                     /locus_tag="ACHJZ2_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO4571091.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAVIGMMLICAGVLVIN
                     LLSRSTPH"
BASE COUNT          465 a          374 c          463 g          432 t
ORIGIN      
        1 ggacgtaacc accgcgacat gtgcgtgctg ttccgctggg catgccagga caacttctgg
       61 tccggtaacg tgctaagtcc ggccaaactc cgtgacaagt ggacccagct cgaaatcaac
      121 cgtaacaagc aacaggcagg catgatagcc agcaaaccaa aactcgacct gacaaacact
      181 gactggattt acggggtgga tttatgaaaa acatcgccgc acagatggtt aactttgacc
      241 gtgagcagat gcgtcggatc gccaacaaca tgccggaaca gtacgacgaa aagccgcagg
      301 tacagcaggt agcgcagatc atcaacggtg tgttcagcca gttactggca actttcccgg
      361 cgagtctggc taaccgtgac cagaacgaac tgaacgaaat ccgccgccag tgggttctgg
      421 ctttccggga aaacgggatc accacaatgg aacaggttaa cgcaggaatg cgcgtagccc
      481 gtcggcagaa tcgaccattt ctgccatcac ccgggcagtt tgttgcatgg tgccgggaag
      541 aagcatccgt tatcgccgga ctgccaaacg tcagcgagct ggttgatatg gtttacgagt
      601 attgccggaa gcgtggcctg tatccggatg cagagtctta tccgtggaaa tcgaacgcgc
      661 attactggtt ggttaccaac ttgtaccaga acatgcgggc caatgcgctg actgacgcgg
      721 aattacggcg caaggctgcc gatgaactga cctgtatgac agcgcgaatt aaccgtggtg
      781 agacgatacc tgaaccagta aaacaacttc ctgttatggg cggtagacct ctaaatcgtg
      841 cacaggctct ggcgaagatc gcagaaatta aagctaagtt cggactgaaa ggagcaagtg
      901 tatgacgggc aaagaggcaa ttattcatta cctggggacg cataagagct tctgtgcaca
      961 ggacgttgcc gcggtaacag gtgcaaccgt aaccagcata aatcaggctg cggctaaaat
     1021 ggcgcgggca ggaatcctgg tcgttgatgg taaggtctgg cgaacggtgt attaccggtt
     1081 cgctaccaga gaagaacggg aaggaaaggt gagcacgaat ctgattttta aggagtgtcg
     1141 ccagagtgcc gcgatgaaac gggtattgag ggtatataaa agaacatcaa tgggtacaca
     1201 atgatgaaac aggtgagttg agttcaaact gtagtacaat tctctccagt ttgaacagga
     1261 aagaatatgt tatgaaccct tatatttatc ttggtggtgc aatacttgca gaggtcattg
     1321 gtacaacctt aatgaagttt tcagaaggtt ttacacggtt atggccatct gttggtacaa
     1381 ttatttgtta ttgtgcatca ttctggttat tagctcagac gctggcttat attcctacag
     1441 ggattgctta tgctatctgg tcaggagtcg gtattgtcct gattagctta ctatcatggg
     1501 ggtttttcgg ccaacggctg gacctgccag ccgttatagg catgatgttg atttgtgccg
     1561 gtgtgttggt tattaattta ttgtcacgaa gcacaccaca ttaaaaataa tttcttttaa
     1621 aagactgcaa tatggcggtt catatcttta catgggccgc ttttgttaat gtttttagtt
     1681 tttgtgtatt cttttgtgcc ttcaagatta ttgcgtaagc aaattgcaat acga
//
LOCUS       DAZFBR010000304         1720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFBR010000304 DAZFBR010000000
VERSION     DAZFBR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.307"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..555
                     /locus_tag="ACHJZ2_005207"
     CDS             <1..555
                     /locus_tag="ACHJZ2_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4571092.1"
                     /translation="ALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGT
                     VIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLG
                     ASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSR
                     RISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(746..1273)
                     /gene="sodC"
                     /locus_tag="ACHJZ2_005208"
     CDS             complement(746..1273)
                     /gene="sodC"
                     /locus_tag="ACHJZ2_005208"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO4571093.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTDKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            1407..>1720
                     /locus_tag="ACHJZ2_005209"
     CDS             1407..>1720
                     /locus_tag="ACHJZ2_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571094.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TIT"
BASE COUNT          398 a          416 c          520 g          386 t
ORIGIN      
        1 gcgctgtcgt tacaggtgcc gggctttcgc cgtcagatga acgaaggctg gtaccagata
       61 cgtattgccg gttatgacac ggcaccggag gcggtgtacg cccgtcttca cgaacagctg
      121 ggtgagggaa cggtcatcca tattgtgccg cgactggccg gggccggaaa gggtggactg
      181 cagattgtgc tgggggcggc agccatcgtg ggctctttct tcactgccgg ggcatcaatg
      241 gcgttatggg gttcagccct ggcagccggt ggtttttctg ccaccacgat gctgttttca
      301 cttggagcca gcatgattct gggcggtgtg gcccagatgc tggccccgaa ggcaaaaaca
      361 ccggattacc gcgcaacgga taacggcaga cagaacacgt acttttcctc gctggataac
      421 atgattgccc aggggaaccc gatgccggtg ccttacggtg aaatgctggt tggctcccgg
      481 cgaatctccc aggacatcag tacccgtgat gaaggcggtg acgggaaggt ggtggttatc
      541 gggcggcagg cataaaagcg aaaaaatccc gcagtgctca cggacaggaa ctgcgggagc
      601 gttacgaaga ttgagtgtaa ggaattattc ttatgtcacg acaaaaacat taactcagag
      661 agggaggatg tgccgttctt ttcagggaga aaggatttat cgtcctgagg aataaaggta
      721 aggggcccgc cccttacctg actgattatt gaatgatgcc gcaggccatt ctcgcaccac
      781 caccgcccag gggctccgga tggtcatgat ggttatcacc gccagcatga agcatgagag
      841 aacgcccttt aatctctttt aatgagttca gtctcggggc caggaccggg tagttcgctt
      901 ttccgtcatg cgtcacgaac agcgcaggga ggtcgcccag gtgtccatcc ggagaccagg
      961 ggccaagatg tttgtcggtg tttttcgggt caaagtgacc gccagccgat aatgctgcga
     1021 ccggttttcc gtctttcagt gccggggcgc aatttccttt ttcgtgcaca tgaaaaccat
     1081 gaatgccttc agacagagag tgaagggctg gtgtgaacag cagaccgtag ggggtctcct
     1141 gaatggttat ttttccaatg ctgacttctt ttccgtcagc actgacaagg ttcattggga
     1201 cttcctgttc tgctgcgtat ccgcatgatg ctgctgtcag catggcaatg gcagcaatga
     1261 ttttacattt cataaaaccc tcattaattc cgttaacaga ctgagcttgc tggttacagg
     1321 gtaacaaaca gcgttctgat gatatcgcgc aatagctgtg caatatccta tcactgcgat
     1381 taataatacc aattgagagg aacattatgg gtaaaggtgg cggcagggcg cacacgccgg
     1441 ttgaggcaaa ggacaatctt aagtccacgc agatgatgag cgtgattgat gccattggtg
     1501 aagggccgat tgaaggtccg gtgaaggggc tgcagagtat cctggtgaac aaaaccccgc
     1561 tgacggacac ggacggtaat cctgtgatac atggtgtgac agcggtctgg cgcgccgggg
     1621 agcaggagca gacaccacct gaaggctttg agtcttccgg agctgaaacc gcactgggcg
     1681 tggaagtgac gaaggcaaag ccggtgacgc gcaccattac
//
LOCUS       DAZFBR010000305         1718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFBR010000305 DAZFBR010000000
VERSION     DAZFBR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.308"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..98
                     /locus_tag="ACHJZ2_005210"
     CDS             <1..98
                     /locus_tag="ACHJZ2_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571095.1"
                     /translation="QGEGFGHELFPVTNKYNAWRWKNKKIIDISW"
     gene            267..1601
                     /locus_tag="ACHJZ2_005211"
     CDS             267..1601
                     /locus_tag="ACHJZ2_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase toxin
                     domain-containing protein"
                     /protein_id="HFO4571096.1"
                     /translation="MTASGTGLGYGEGDESYGYDSCGYLKAQSAGGHRISEETDQYAG
                     GHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGEQWEY
                     RHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDELYSVRHLVFNGFELISQQC
                     SRVRQPHPSVAPQWVTRTNHAVNDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPAD
                     TDYPDPRGERDAEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLQGGE
                     QTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTGFLDGVRLSRSQINNIA
                     KEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLYEVYHEVIHAKQFAKIG
                     REAYEALGRLSREEHVLNEILKSKNLFNEAEIAHAIKYVEGLREKFMMGLIN"
BASE COUNT          487 a          373 c          509 g          349 t
ORIGIN      
        1 ggcaagggga aggttttggc catgaattat tccccgtgac aaacaaatat aatgcttgga
       61 gatggaaaaa taaaaaaata atagatattt cttggtaagg aaaatattcc ccgaaagagg
      121 ccacatgggg aatactgctc ccctcactga tacgggagta ccgttacgac agcgcgggta
      181 acgtatcagg cgtcaccagc cgggaagatt acggacggga aacacagcgg gagtaccggc
      241 ttgaccggaa cggccaggtc acggcggtga cagcctcagg caccgggctg ggctatggcg
      301 aaggcgatga gtcctatggc tatgacagct gtggctacct gaaggcgcag tctgcgggcg
      361 ggcaccggat aagtgaagag actgaccagt atgccggagg ccaccggctg aaacaggccg
      421 gaaacacgca gtatgactat gacgccgcag gccggatggt cagccgcacc agacaccgtg
      481 acggctatcg cccggaaaca gagcggttcc ggtgggacag ccgggaccag ctgaccgggt
      541 attgcagcgc acagggtgag cagtgggaat accgccacga cgccagcggc agacgaacag
      601 agaaacgctg cgaccggaag aaaatccgct tcacgtacct gtgggacggc gacagtattg
      661 cggaaatccg ggaataccgc gatgatgaac tgtacagcgt acggcacctg gtgtttaacg
      721 gctttgagct gataagccag cagtgcagcc gggtacggca gccgcacccg tccgtggccc
      781 cgcagtgggt gacacgaacg aatcatgcgg tgaacgacct gacgggccgt ccgctgatgc
      841 tctttaacag tgaaggtaaa accgtctggc ggccgggaca gaccagcctg tgggggctgg
      901 cactcagcct gcccgcagac acagactacc cggacccgcg cggggagcgg gacgcggaag
      961 ccgaccccgg cctgctgtat gcaggacagt ggcaggatgc agaatcgggg ttgtgctata
     1021 accggttccg atattacgag ccggaaaccg ggatgtacct ggtgagtgat ccactggggt
     1081 tgcagggagg ggagcagact taccggtatg tgccgaatcc ttgtgggtgg gttgatccgc
     1141 tgggattggc tgcaagttct aaaatcagca gtttgatgga ctatattggc gatggtcgtc
     1201 gtgttagtgg gcatacgggt ttcctggatg gggttcgttt atcacgtagt caaataaaca
     1261 atattgctaa agaaatggag aagctaggaa ttaaagtaat aaggaaagca gataaatatt
     1321 tgccaccaaa tgctagggca gcttttgatt atggtcttcg caatatttat cttaggaaaa
     1381 atgctacctt atatgaggtg tatcatgaag tgattcatgc taagcaattt gcgaaaattg
     1441 gacgagaagc atacgaagca ctaggacgtt tatctaggga ggaacacgtt ctaaatgaaa
     1501 tattaaaaag taaaaattta ttcaatgaag cggaaatagc tcatgccata aaatatgttg
     1561 agggattgag agaaaaattc atgatgggac taataaattg ataaaacata acaaaataac
     1621 tatcgaaatg gctcttgact tggctcggcg tgagttagag ttaagggaaa ttccttatat
     1681 taaaaatagt ctacatgcca attacagcta taaatcta
//
LOCUS       DAZFBR010000306         1690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFBR010000306 DAZFBR010000000
VERSION     DAZFBR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.309"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>1690
                     /locus_tag="ACHJZ2_005212"
     CDS             <1..>1690
                     /locus_tag="ACHJZ2_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4571097.1"
                     /translation="GAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
BASE COUNT          522 a          396 c          438 g          334 t
ORIGIN      
        1 ccggggctgt cactgttgcc attcgccgcg aatacaaaca gacatggaaa acactcgaca
       61 atgaactggc ctgcgccctc tggcccggtg atgtggatgc aggaaacatt gacggtacca
      121 tccatcgctg ggcgacaaat gaggttatcg acaaggatcg cgaagactgg aagcgtatct
      181 cagcatcaat gcgcaaacag cccgaagcac ttggctatga ccgtcagact atttttggcc
      241 ttgttcgcga acgtccgatc gatattcaca aagatcccgt tgcactgaac aaatatatca
      301 gtgaatacct gacgacaaag ggcgtgtttg aacatgaaga aacagaccag agctctactg
      361 atgctctcca gccgtcagca gcacaaactg ctccagtgga gacggcagaa tccgatactc
      421 aaaaaaatga aatcctggtg gaagctgaac catctgtaga gcgtgaagga ccattttatt
      481 tcgtctttac cgataagggc ggggaaaaat acggcagggc aaacaaactt tctggtctgg
      541 acaaggcgct ggctgccggc ggtaccgaaa tctcaaaaga agaatatttt gcccgaaaaa
      601 atggcacata cacgggctta ccgcaaaatg tggataccgc tgaagattcc gaacaatcag
      661 agccggtaaa agttaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc
      721 agcctgacgc cgataaattg cttgctgcat cacgtggtga atttgttgaa gggattagcg
      781 acccgaatga tccgaaatgg gtgaaaggga tccagagtcg cgacgctgag gaccagaatc
      841 agcccaacgt gaaacaaaat gagccagaag cggaacaaaa cagcccggat acgcaacaaa
      901 acgggccaga agaacaacaa ccagaaccag cagtgcaaca ggaactggaa aaagtttgca
      961 ccgcatgcgg tcagaccggt ggcggcaact gtcctgactg tggtgcggtg atggggaacg
     1021 caacctacct ggaaacattc gatgaagaga atcaggctga agctcagaaa aatgatccgg
     1081 aggaaatgga aggcactgaa catctgcaca aggagaacac tggcagcgat cagtatcacg
     1141 ccagcgataa taaaactggc gaaacagcaa atcccttaat taaagtgaac ggtcatcatg
     1201 aaatctcatc caccagcagg ttgtggcacc atctgatgat tgaccttgaa acaatgggaa
     1261 aaaatcctga tgcgccaata aactctatag ccggtaagtt ttttgatccg gcaaccggag
     1321 agatggggcc agaattcagc aaaactatcg atctggaaac cgcaggtggg gtcatcgatc
     1381 gggacaccat taagtggtgg ctgaaacagt cacgcgaagc acaatcctcc attctgaccg
     1441 atgaaatcac gttggatgat gcactgctgc aattccggga atttatcgac gaaaactccg
     1501 gtgaattttt tgttcaggtc tggggtaacg gtgcaacttt cgacaacgtg attttacgcc
     1561 gttcatatga acggcagggg atcccctgcc cgtggcgtta caccaatgat cgcgatgtaa
     1621 gaacgatggt tgctctggga ctggtgatgg atttcgacgc aagaacgact attccattcg
     1681 aaggtgaacg
//
LOCUS       DAZFBR010000307         1680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFBR010000307 DAZFBR010000000
VERSION     DAZFBR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.310"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..598
                     /gene="wzx"
                     /locus_tag="ACHJZ2_005213"
     CDS             <1..598
                     /gene="wzx"
                     /locus_tag="ACHJZ2_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFO4571098.1"
                     /translation="LQLGTLATWSGDNFIISITLGVTYVAVFSITQRLFQISTVPLTI
                     YNIPLWAAYADAHARNDTQFIKKTLRTSLKIVGISSFLLAFILVVFGSEVVNIWTEGK
                     IQVPRTFIIAYALWSVIDAFSNTFASFLNGLNIVKQQMLAVVTLILIAIPAKYIIVSH
                     FGLTVMLYCFIFIYIVNYFIWYKCSFKKHIDRQLNIRG"
     gene            601..>1680
                     /gene="rfbE"
                     /locus_tag="ACHJZ2_005214"
                     /gene_synonym="perA"
     CDS             601..>1680
                     /gene="rfbE"
                     /locus_tag="ACHJZ2_005214"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFO4571099.1"
                     /translation="MKYIPVYQPSLTGKEKEYVNECLDSTWISSKGNYIQKFENKFAE
                     QNHVQYATTVSNGTVALHLALLALGISEGDEVIVPTLTYIASVNAIKYTGATPIFVDS
                     DNETWQMSVSDIEQKITNKTKAIMCVHLYGHPCDMEQIVELAKSRNLFVIEDCAEAFG
                     SKYKGKYVGTFGDISTFSFFGNKTITTGEGGMVVTNDKTLYDRCLHFKGQGLAVHRQY
                     WHDVIGYNYRMTNICAAIGLAQLEQADDFISRKREIADIYKKNINSLVQVHKESKDVF
                     HTYWMVSILTRTAEEREELRNHLADKLIETRPVFYPVHTMPMYSEKYQKHPIAEDLGW
                     RGINLPSFPSLSNEQVIYICESINEF"
BASE COUNT          570 a          257 c          315 g          538 t
ORIGIN      
        1 tttacagtta ggcactctgg caacatggag tggtgataac tttataatat ctataacatt
       61 gggtgttact tatgttgctg tttttagcat tacacagaga ttatttcaaa tatctacggt
      121 ccctcttacg atttataaca tcccgttatg ggctgcttat gcagatgctc atgcacgcaa
      181 tgatactcaa tttataaaaa agacgctcag aacatcattg aaaatagtgg gtatttcatc
      241 attcttattg gccttcatat tagtagtgtt cggtagtgaa gtcgttaata tttggacaga
      301 aggaaagatt caggtacctc gaacattcat aatagcttat gctttatggt ctgttattga
      361 tgctttttcg aatacatttg caagcttttt aaatggtttg aacatagtta aacaacaaat
      421 gcttgctgtt gtaacattga tattgatcgc aattccagca aaatacatca tagttagcca
      481 ttttgggtta actgttatgt tgtactgctt catttttata tatattgtaa attactttat
      541 atggtataaa tgtagtttta aaaaacatat cgatagacag ttaaatataa gaggatgaaa
      601 atgaaatata taccagttta ccaaccgtca ttgacaggaa aagaaaaaga atatgtaaat
      661 gaatgtctgg actcaacgtg gatttcatca aaaggaaact atattcagaa gtttgaaaat
      721 aaatttgcgg aacaaaacca tgtgcaatat gcaactactg taagtaatgg aacggttgct
      781 cttcatttag ctttgttagc gttaggtata tcggaaggag atgaagttat tgttccaaca
      841 ctgacatata tagcatcagt taatgctata aaatacacag gagccacccc cattttcgtt
      901 gattcagata atgaaacttg gcaaatgtct gttagtgaca tagaacaaaa aatcactaat
      961 aaaactaaag ctattatgtg tgtccattta tacggacatc catgtgatat ggaacaaatt
     1021 gtagaactgg ccaaaagtag aaatttgttt gtaattgaag attgcgctga agcctttggt
     1081 tctaaatata aaggtaaata tgtgggaaca tttggagata tttctacttt tagctttttt
     1141 ggaaataaaa ctattactac aggtgaaggt ggaatggttg tcacgaatga caaaacactt
     1201 tatgaccgtt gtttacattt taaaggccaa ggattagctg tacataggca atattggcat
     1261 gacgttatag gctacaatta taggatgaca aatatctgcg ctgctatagg attagcccag
     1321 ttagaacaag ctgatgattt tatatcacga aaacgtgaaa ttgctgatat ttataaaaaa
     1381 aatatcaaca gtcttgtaca agtccacaag gaaagtaaag atgtttttca cacttattgg
     1441 atggtctcaa ttctaactag gaccgcagag gaaagagagg aattaaggaa tcaccttgca
     1501 gataaactca tcgaaacaag gccagttttt taccctgtcc acacgatgcc aatgtactcg
     1561 gaaaaatatc aaaagcaccc tatagctgag gatcttggtt ggcgtggaat taatttacct
     1621 agtttcccca gcctatcgaa tgagcaagtt atttatattt gtgaatctat taacgaattt
//
LOCUS       DAZFBR010000308         1673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFBR010000308 DAZFBR010000000
VERSION     DAZFBR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.311"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..572
                     /locus_tag="ACHJZ2_005215"
     CDS             <1..572
                     /locus_tag="ACHJZ2_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO4571100.1"
                     /translation="DEAVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDLQY
                     FKLQLLDASNPRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQA
                     IETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNVNL
                     KNQDTNNSSFSYGDNRFEKSQGSKWTFQE"
     gene            586..825
                     /locus_tag="ACHJZ2_005216"
     CDS             586..825
                     /locus_tag="ACHJZ2_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFO4571101.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            845..1177
                     /gene="sctI"
                     /locus_tag="ACHJZ2_005217"
     CDS             845..1177
                     /gene="sctI"
                     /locus_tag="ACHJZ2_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO4571102.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALTSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            1174..>1673
                     /gene="sctJ"
                     /locus_tag="ACHJZ2_005218"
     CDS             1174..>1673
                     /gene="sctJ"
                     /locus_tag="ACHJZ2_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFO4571103.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAILQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIK"
BASE COUNT          580 a          283 c          333 g          477 t
ORIGIN      
        1 tggatgaagc agtctgggtc aaacaagcac tggaaaaaaa tcaaccagga aagccagtac
       61 gggtgataaa tcctgatgat gaatcgatac gaattttttc atggcttgct gataatttcc
      121 ctgatttaca atattttaaa cttcagctat tagatgccag taatcccaga ctgaccgtga
      181 gtaagcaacg gaatgctatc acacagcaac taatcgacaa tcttattaaa gggttactac
      241 aaactatgcc atatgccagc aatataagta ttgcggtatt agatgataat gtattggaaa
      301 gtcaggctat tgaaacattg tctgcaatag gtctttctta tgaaaaatat aaaacagcta
      361 acaatgtgta tttcaatatc attggtacgt taagcgacag tgaattaaac aaaataaata
      421 actatgttga cgaatattat aaacaatggg gtaaacaata tgtaagattt aatgtgaatt
      481 tgaaaaatca ggacacaaat aatagttcat ttagctacgg agataaccga ttcgagaagt
      541 ctcaaggtag caagtggaca tttcaggaat aattgaggag gacttatggc agattggaat
      601 ggttatatta tggacatcag caaacaattt gatcagggcg ttgatgatct gaaccagcaa
      661 gttgaaaaag cgttggagga tttagcaacc aatccttccg acccgaaatt ccttgccgaa
      721 tatcaaagtg cattagctga gtatacatta tatcgaaatg cgcaatctaa cgttgtaaaa
      781 gcgtataaag atcttgattc ggcaatcata caaaacttcc gctaaactgg ataaggattt
      841 tattatgtca gtaagcaata tgccacctat tgatagagct gagcaatcaa ctgctcatga
      901 aatacagcaa gcgaaagtga ttgatttaaa tgaccgggtt ctcaatcttg acaacccgga
      961 tgataaaatg atctccgctt ttgctaacta tgctgtacaa actgaaaatt ggcagcaaaa
     1021 tgcgttacaa gcattaacgt ctgataaaaa aggattaact cctgaaaaat tattagtctt
     1081 gcaggatcat gtgcttaatt ataatgtcga ggtttcattg gtgggaacat tagcaagaaa
     1141 aattgtcgct gcagttgaga cgttgacccg ctcatgaaat atatatcatt attattgttt
     1201 atcctgctgt tgtgcggctg caagcagcag gaattactga accatcttga tcaacagcaa
     1261 gctaatgatg tccttgctat tttacaaaga cataatatta atgctgaaaa gaaggatcaa
     1321 ggcaaaactg gattctcaat ctatgttgaa ccaacggatt ttgcttctgc ggtggattgg
     1381 ctaaaaatct acaatctccc gggtaagcct gatattcaaa tatcgcagat gttccctgca
     1441 gatgcactcg tttcatctcc acgagcggaa aaagcaaggc tatattctgc tattgagcag
     1501 cggttagagc aatctctcaa aattatggat ggtattgttt cttcgagagt acatgttagt
     1561 tacgatgttg atactggcga tagtggtaaa actgctttac caattcatat ttctgtactt
     1621 gcagtatatg aaaaagatat caatccagaa ataaaaataa atgacataaa gag
//
LOCUS       DAZFBR010000309         1630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFBR010000309 DAZFBR010000000
VERSION     DAZFBR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.312"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..257
                     /locus_tag="ACHJZ2_005219"
     CDS             <1..257
                     /locus_tag="ACHJZ2_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4571104.1"
                     /translation="PKGDPGETQIRFRLGPGNIIETNSHGWFPDTDGALITGLTFLDP
                     KDATRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            259..528
                     /locus_tag="ACHJZ2_005220"
     CDS             259..528
                     /locus_tag="ACHJZ2_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4571105.1"
                     /translation="MNILKKIMQRLCGCGKHDDRENGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            642..1217
                     /locus_tag="ACHJZ2_005221"
     CDS             642..1217
                     /locus_tag="ACHJZ2_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4571106.1"
                     /translation="MPLTSAIASNSFSTGMQVLRAQMAASGGGEITVGGQTVRITYSE
                     TDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCK
                     FSVNTESLQCPSEAVRCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHP
                     LTREPITSSMIVSQEQCIYDQTKGNFVIKDK"
BASE COUNT          448 a          291 c          467 g          424 t
ORIGIN      
        1 ggccgaaggg cgacccgggg gagacgcaaa tacggttccg tctggggccg ggaaacatta
       61 ttgagacaaa cagccatggc tggttcccgg atacagatgg cgcactcatc accggactga
      121 cctttcttga ccccaaagat gccacacggg ttcagggttt ttttcagcat ttgcaggtca
      181 ggtttggtga cgggccgtgg caggatgtta aggggctgga tgaagtgggc agtgatacag
      241 gcagaacagg agaatgacat gaacatatta aaaaaaatta tgcagcgtct gtgcggttgc
      301 ggaaagcatg atgaccgtga aaacggggag ttacttacag cacagctgcg actgggaccg
      361 gcagacattc tggagtccga tgagaatggt attatcccgg agcaggacag ggtaatcacg
      421 caggtggtga tactggatgc ggataaaaag cagatacagt gcgtggtaag accgctgcaa
      481 atcctgcgtg ctgacgggac gtgggaaaat attggcggga tgaaatagcc cgacagcttc
      541 acaaaaaccg gagtccggct ccggtttttg ttgtcatgta tgggggctgt ttgttatgac
      601 tccctgtgtt tggaatgaat atttaaatag ggagttttgt catgccgtta acatcagcta
      661 ttgcatccaa ttcattttcc accggaatgc aggttcttcg tgctcaaatg gccgccagtg
      721 gcggtggaga aattacagta ggcgggcaga cggtccgtat cacatatagt gaaacggatg
      781 gtcgctttct ggcgagtggg ggcaataaca gtttgctttc tggattatta cttacagggc
      841 ttaatggtgg tcctgaagcg ctcagggata taatgttaag aatggtttca ggttcaggta
      901 acacacaatc acatggtgat attgagggga aaatatcaca atgtaagttt tctgttaata
      961 cggagagcct tcagtgtcca tccgaggcgg ttcgatgccc aattatactg gataaaccag
     1021 aagaaggtgt gtttgttaaa aattcagaag gttctttggt ttgtacctta tttgattcgg
     1081 tttctttttc tcatttggtt cgcgacggtg ggaagcaccc gctaacacga gaaccaataa
     1141 cgtcatcaat gattgtaagt caagaacaat gtatttatga ccaaaccaaa ggaaactttg
     1201 tcataaagga taagtgaaat aaatattacc caagctatat gttaactgcc agttgcttat
     1261 atgaaatgct acagatgttc agggtataag gatgtggtaa ttggtgtact ggatgcagct
     1321 aaaaagcata tacagtgagt ggtgaggccg ctgcaaatct ggcgtgctga cggggaaata
     1381 ttggcgggat gaaatagccg acggattcac aaaaaccgga gtccggctcc ggtttttgtt
     1441 gtcatgtcag ggagatgttt gttatgaagc cagaggaaat atttatctgt atgaaggaat
     1501 atggtaatgc ctggattagt atcatatata tcatcgactt cattcgcgaa tgagatggcg
     1561 gagatgcgtc agcaggtaat ggaagggcag attggtggat ttctcctggg aggggagaga
     1621 gttagagttt
//
LOCUS       DAZFBR010000310         1609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFBR010000310 DAZFBR010000000
VERSION     DAZFBR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.313"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>1609
                     /locus_tag="ACHJZ2_005222"
     CDS             <1..>1609
                     /locus_tag="ACHJZ2_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4571107.1"
                     /translation="QSFQSEIQRASRMGSEYYRTLQNGGRQAAAAAREQRRALAELNS
                     QLTEIRGSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQASQSSDEFSSSQKVLMD
                     ISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKISGASTAEAGSVI
                     TQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLKAMADDGKLTADKVVP
                     ALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASGATKTLSGVLNGVAGN
                     IDNVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVALAEAQLRGTQIATAR
                     ARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARTAAQNALNSTTAVGSR
                     LMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDEIAHKTPSMSLPE
                     ASDNEGRTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTGDGFMINHLTS
                     VKTVTEGLSQATEQLAVEQSRLAQMQEK"
BASE COUNT          363 a          397 c          542 g          307 t
ORIGIN      
        1 cagtcattcc agtcggagat ccagcgggcg tcccgtatgg gcagtgaata ttaccggacc
       61 ctgcagaatg gcgggcgtca ggccgctgcg gcagcccggg agcagcgacg tgccctggca
      121 gaactgaaca gccagttgac ggaaattcgc ggttctgctg tcggaatggc tggcgcattt
      181 gccggtgcct ttgccaccgg acacctgatt tctctggccg atgaatggag ctccgtgaat
      241 gcccgtctga aacaggcgtc gcagtcatct gatgaatttt cgtcatcaca gaaagtgctg
      301 atggatatca gccagcggac aggcacggca ttttcggata atgcggccct gtttgcccgt
      361 tcggctgcct cgatgcgtga atatggttac agtgctgatg atgtgctgaa ggtgacggag
      421 gccatttcca cggggctgaa aatctccggt gccagtacgg cagaggcggg ctcggtgatc
      481 acccagttca gtcaggcgct ggcgcagggt gtattgcgtg gtgaggagtt taattcggtc
      541 aatgaaagtg gtgaccggat cgtacgtgca ctggctgcgg gtatgggcgt ggcccgtaaa
      601 gatctgaagg caatggcgga tgacggaaaa ctgacagcgg ataaagtggt ccccgcgtta
      661 atcagccagc tggggatatt acgtgatgaa tatgccgcca tgccggaaac ggtctctgac
      721 gggatcacaa aggtggaaaa cgcctttatg gcctgggtgg gtggcgcgaa tgaagccagc
      781 ggagcgacga aaacgctctc cggcgtgctg aacggtgttg ccggtaatat tgataatgtg
      841 gcaacagccg cgggggcgct ggttgccgtc ggggtggccc ggtactttgg caatatggcc
      901 tccggagcgg tgtctgccac ggcaggactt gtgacggcag cacgtaatga agtggcactg
      961 gcggaagcac agctcagggg gacgcagatt gccacggcgc gggcaagggc agccgtgtac
     1021 cgtgcacagc aggctgtggc ggcagcccgc gggacggaga tgcagattgc tgcagaagcc
     1081 cgtctggcgg ccacacagga acgcctgaac agaaatattg ctgccagaac cgcagcccag
     1141 aatgcgctga acagtacaac ggcggtgggc tcacgtctga tgactggtgc gttgggactg
     1201 gttggtggcg tacccggact ggtgatgctg ggggcagcag catggtatac gctgtaccag
     1261 aatcaggagc aggccaggga gtcagcgcgc cagtatgcac tgacgataga tgaaatcgcg
     1321 cataaaacgc cgtcaatgtc tttgcctgaa gcctcagata atgaaggacg aacacgggcg
     1381 gcgctggcag agcagaaccg gctgattgat gaacaggcca gccgggtgaa atccctgcag
     1441 gaaaaaatcg ctggatatca gtatgttctg gctaaccctg gctggacaac cggtgacgga
     1501 ttcatgataa accatctgac atcggtgaag accgtgacgg aagggctttc tcaggcaaca
     1561 gagcagcttg ccgttgagca gtcccgtctg gcacagatgc aggaaaaag
//
LOCUS       DAZFBR010000311         1605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFBR010000311 DAZFBR010000000
VERSION     DAZFBR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.314"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..218
                     /locus_tag="ACHJZ2_005223"
     CDS             <1..218
                     /locus_tag="ACHJZ2_005223"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4571108.1"
                     /translation="LAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEIN
                     IVRAQPVSGGRLGVKIYPIMLEGQVKK"
     gene            215..1036
                     /locus_tag="ACHJZ2_005224"
     CDS             215..1036
                     /locus_tag="ACHJZ2_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4571109.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDFRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1274..1605
                     /locus_tag="ACHJZ2_005225"
                     /pseudo
     CDS             1274..1605
                     /locus_tag="ACHJZ2_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310217.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          428 a          315 c          475 g          387 t
ORIGIN      
        1 ggctggcgat aaagattatt gccgagccac cggataagcg ccgccgtgac ctggacaata
       61 ttctgaaagc gccgctggat gcgctgacgc atgcggggtt gctaatggac gatgagcagt
      121 ttgatgaaat caatatcgtt cgtgctcagc cagtatctgg tggacgtctg ggggtgaaga
      181 tttaccccat aatgcttgaa gggcaggtca aaaaatgaaa ctggaagatt taccgaaata
      241 ctactcccca aaatcccccg gcctgactga tgcatcggcc tcaacgtcga aagatacgct
      301 gagtatcact gatgtgatgg ccgcgcaggg catgacacag aattgggctg agatggggtt
      361 ttctgcgttc cttgggaaaa tgggcattag tatgaatgac agagagcggg caacagaatt
      421 gctgacagaa tatgcactca gtcggtgtga tcgcgtggcg gcgttaagaa aactcccggc
      481 agaaataaaa ccggcagtga tgcgtattat ggcttcgtat gcgtttgaag attatgcccg
      541 tagcgcggcg agcaaaaaac agtgcccctg ttgtcacgga aaaaaattta ttgaaagcga
      601 ggtttttaca aacaagatcc agtatccgga tggtaagccg ccagtgtggg caaagtgcac
      661 aaaaggcgtg tatccgtctt actgggagga atggaaaaaa gtcagggagg tggtaaaagt
      721 tgcctgtccg gagtgtggag ggaaggggga ggtttccacc gcctgtaaag attttcgtgg
      781 gcgcggtgtt gccattcatc gtgaagagtc ggtaaaacgt ggtatgcctg ttatcagaga
      841 ctgccagcgt tgtggtggtc gtggctatga aagattacct tcaacggagg catttaatgc
      901 catatgtaat gtaaccgatg ccatatctct tgatacatgg aaaaaaacag ttaaacgttt
      961 ttacgatacg ctggtggtgc agtttgatat tgaagaagca tgggcagaac aacaactgaa
     1021 aaaggtgacc agatagcttt gttgattttt cccgaatctg tggtaaattt gccctaacga
     1081 tgggcgtttt atgcctgacg ttagaagatt ttttacaccc gtcgccaggc gggttttttt
     1141 atgactgaaa tcacgccagt acagtaaacg cgctggtggt tgtgaatacc ggtctttctg
     1201 cttgctggct ttttcgacaa gagttattgg tgtgtcacgt taaccggaaa aaggaaagtt
     1261 tgagaaacgc gatctggcac aggcggtgat taacgccgca tacctggtgg cctgtgcaga
     1321 tggtgaatgt gaggcttcct agaaagcgaa gatcgaacag gtactgcgta atcagccagc
     1381 gctgtccgcg tttacgtcag aaattaatgc gattagcgca accattatcg gtcagctgga
     1441 tacgaacttt aaaactggtc gtcgtgcggc gttacgtgag atcgaggatg tgaaacacga
     1501 tacgcgtgaa gcggaagatg tgctggatgt ggcggtggcc attgcggagg cagacggcga
     1561 aattgagccg gaagagcgca aggtgctgga agagattgcc ggtgt
//
LOCUS       DAZFBR010000312         1592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFBR010000312 DAZFBR010000000
VERSION     DAZFBR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.315"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..491)
                     /locus_tag="ACHJZ2_005226"
     CDS             complement(<1..491)
                     /locus_tag="ACHJZ2_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571110.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSVFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLA"
     gene            complement(493..771)
                     /locus_tag="ACHJZ2_005227"
     CDS             complement(493..771)
                     /locus_tag="ACHJZ2_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571111.1"
                     /translation="MVHYKKLNNSDNSAVFASRHGRCSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISARLPYSERPPRPSRTFQQRIWR"
     gene            complement(837..1004)
                     /locus_tag="ACHJZ2_005228"
     CDS             complement(837..1004)
                     /locus_tag="ACHJZ2_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571112.1"
                     /translation="MNDKELIAALSIPGNYEVIVLGNGEFIVMPLPSDVILISKESHA
                     DSVSYFSIKRD"
     gene            complement(1283..1438)
                     /gene="hokD"
                     /locus_tag="ACHJZ2_005229"
     CDS             complement(1283..1438)
                     /gene="hokD"
                     /locus_tag="ACHJZ2_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4571113.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          400 a          423 c          381 g          388 t
ORIGIN      
        1 gccagcgata actccctctc ctgcgtaccg cttattgcgt gacggatgac atcaatcatc
       61 caggcaacca gattctgctg agcaagttga tcgagtgatt ctgatgtctg gtcgcgcagc
      121 tggttgtcgc agtgccagca caacaccatc gcgccggtac cataacggtg aatgactgtt
      181 tcgctgtgat gataatcgcc gtgtggccac tggcaggatt tcacgtgacg taataaccag
      241 tcagacagtg cacctgcacc acctgctgca cgaataaccc gctcatcgct gaaaaatggc
      301 agtaatgttt tatcctctgc cagcggctgg cgaacggcag gaacgactcc ggacggcaga
      361 ccgcgcatgt ttttcggttc cggctccacc aatattctgc cgttatggaa tactgacatt
      421 gattcacggc ctggcttaac gataaccaga ccgagttccg gtaccagaac aggtcgaagt
      481 aatacccgca cgttacctcc agatgcgctg ctggaatgtg cgggacggac gcggtgggcg
      541 ttcggaataa gggagcctgg cggagattat ccagtgacga cgatcgaagc tgagatcttt
      601 ctgaaactcg taaccacgcc tgcggtagca ctgaatcagc cattcggcct gttcttcagt
      661 gcatgggtca tgctggaacc agtcagattt gaatgcatga gaacaccgcc cgtgcctgct
      721 ggcaaagacg gctgaattat cagaattgtt aagtttcttg taatgcacca ctactatcct
      781 cgtaatggtg cgacagacgc cagttgttca ggctggcttt actacagcat tataatctag
      841 tctcttttaa tgctgaagta actgaccgaa tccgcatgcg attctttgct gatcagaata
      901 acatcagatg gcaatggcat tacgataaac tcaccattcc caagaactat tacttcataa
      961 ttacctggta tagaaagcgc agcaattaat tccttatcgt tcataacaaa aatcccatga
     1021 actataaaca cctcccctta tgggggccat ccctcttatc cctgcgcgct acttaagagc
     1081 ctcgattcta gccgtgaaga catgccaatc aacagcaata aaccacctga ataacagaat
     1141 cagccagcca catcggtaca accagatgca tcacgctcac cgaaaataaa caacaaaaaa
     1201 ccgccgaagc gagttaagtg gggtgcgttg aggatgcctg acacatcaga ggtggcgagg
     1261 gatttctccc ccgccaggtc tcttactcct caggttcgta agctgtgaag acagcgacct
     1321 ccgtctggcc ggttcggatt cgtacctcgc agaggtcttt cctcgttacc agtgccgtca
     1381 ctatgacggt taaacagatg acgatcaggg cgattagcat cgccttttgc tgcttcatag
     1441 cctgcttctc cttgcctttc ggcacgtaag aggctaacct acgtgtgtag agcatagata
     1501 tggcctcaga ttaatgttaa gcgtcttgcc ggacgcgtaa tgttaactgg ggcttttctc
     1561 tatctgccgt tggtgttcat gcccgaggca ga
//
LOCUS       DAZFBR010000313         1586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFBR010000313 DAZFBR010000000
VERSION     DAZFBR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.316"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1047
                     /locus_tag="ACHJZ2_005230"
     CDS             <1..1047
                     /locus_tag="ACHJZ2_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO4571114.1"
                     /translation="AHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDGDKTYARLGFK
                     GETQINDQMIGFGHWEYDFKGYNDEANGSRDNKTRLVYAGLKISEFGSLDYGRNYGVG
                     YDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDFFGLIEGLNFAAQYQGKNE
                     RTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASG
                     KNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRP
                     SLAYLKSKGIDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDI
                     RTDNIVATGITYQF"
     gene            1210..>1586
                     /locus_tag="ACHJZ2_005231"
     CDS             1210..>1586
                     /locus_tag="ACHJZ2_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO4571115.1"
                     /translation="MIMTNINTACVKNNASYQFNNALPNKETISSNFCERLEQWGNKS
                     LNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFT
                     DNASLVNINLSFNKINTITFPNES"
BASE COUNT          470 a          334 c          325 g          457 t
ORIGIN      
        1 gcacacgctg ccgaagtcta taacaaggat ggtaataaac tcgaccttta tggcaaggtt
       61 accgctctac gttattttac tgatgataag cgtgacgatg gtgataaaac ttatgcccgt
      121 ctcggcttta aaggagaaac gcaaatcaat gatcaaatga ttggttttgg tcactgggaa
      181 tatgatttta aaggctataa cgatgaagcc aacggctcgc gcgacaacaa gacccgtctg
      241 gtctatgctg gtttaaaaat tagtgaattt ggctctctgg actatggccg taactacggt
      301 gtcggctatg acattggttc atggactgat atgttgccag aatttggtgg cgatacctgg
      361 agtcagaaag atgtcttcat gacataccgt accaccggtg tagcaaccta tcgcaactac
      421 gatttctttg gcttaattga agggctgaac tttgccgcgc aatatcaagg caaaaatgaa
      481 cgtactgaca acagtcatct ttatggtgct gactacacgc gtgccaacgg tgacggtttc
      541 ggtatctcct caacttatgt ttatgatggc tttggtatcg gagcggtgta taccaaatcc
      601 gatcggacaa atgcgcagga aagagccgct gctaatcctc tcaatgcctc cggtaagaat
      661 gcagaactgt gggctacagg tataaaatat gatgccaaca acatctactt tgcagctaat
      721 tacgctgaaa cattaaacat gaccacctat ggcgatggtt atatttctaa caaagcacaa
      781 agttttgaag tggtggcgca atatcaattc gacttcggct tgcgcccctc actcgcttac
      841 ctgaaatcga aaggcataga tctgggccgc tacggcgatc aggacatgat tgagtatatc
      901 gacgttggtg cgacgtattt cttcaacaaa aatatgtcga cctatgttga ttataaaatc
      961 aacctgattg atgaaagcga ctttacccgt gccgtagata ttcgcaccga taacatcgtc
     1021 gctacgggca ttacctatca gttctaacat cctctcctga ccagcccttc tctgggctgg
     1081 tttttgattt aagtgctgct gtatgactcc ttaagtaaaa atgagcaatt tttgatattt
     1141 ctggtcattc acctcaatta atattattcc atacccccgg ggacatctta ctataatatt
     1201 tattgatgag tgattatgac caatattaat acagcttgtg taaaaaataa tgccagttat
     1261 caatttaata acgcattacc taacaaagaa accatctcca gcaatttttg tgaacgacta
     1321 gaacaatggg gtaataagtc gcttaataat ggtgaagaaa gagcaattgc cgtggagcga
     1381 attaaagaag cttacaattc gaacatggca tctcttgatt tgtcatatct tgatttgagt
     1441 gagttaccgc ctatcccctc gacagttaat acgttaaatc tggaaaacaa ttgtctaact
     1501 tgtcttgact ttactgacaa tgccagtctc gttaatatca acctcagctt taataaaatt
     1561 aacacgataa cctttccaaa tgaatc
//
LOCUS       DAZFBR010000314         1583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFBR010000314 DAZFBR010000000
VERSION     DAZFBR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.317"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>1583
                     /gene="rhsC"
                     /locus_tag="ACHJZ2_005232"
     CDS             <1..>1583
                     /gene="rhsC"
                     /locus_tag="ACHJZ2_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFO4571116.1"
                     /translation="PGGVTSGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRT
                     KTPAPVGSLGPGWKMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWL
                     VRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAE
                     EARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYG
                     WTPRGELAVVYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNP
                     AGLSYTYQYEKDRITITDSLNRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRL
                     RAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREY
                     DEWGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSG
                     YVTRYDHDRFGQMTAVHRE"
BASE COUNT          328 a          482 c          510 g          263 t
ORIGIN      
        1 gccccggcgg agtgacgtcc ggccatccgg tcaatcccct gctcggtgca aaggtccttc
       61 ccggtgaaac cgacatcgcc ctgcccggcc cgctgccgtt catcctctcc cgcacctaca
      121 gcagttaccg gacaaaaacg cccgcgccgg tggggagcct cggccccggc tggaaaatgc
      181 ctgcggatat ccgcttacag ctgcgcgata acacactgat actcagtgat aacggcggca
      241 gaagcctgta ttttgagcac ctgtttcccg gtgaggacgg ttacagccgc agcgagtcac
      301 tctggctggt gcgcggcggc gtggcgaaac tggatgaagg tcaccggctg gccgcactct
      361 ggcaggcgct gccggaagaa ctccgcttaa gtccgcatcg ttatctggcg acaaacagtc
      421 cgcaggggcc gtggtggctg ctcggctggt gtgagcgggt gccggaagcg gatgaggtgc
      481 tgcctgcgcc gctgccgccg taccgggtac tgaccgggct ggtggaccgc ttcgggcgca
      541 cacagacgtt ccaccgcgaa gccgccggtg aattcagcgg cgaaatcacc ggcgtgacgg
      601 atggtgccgg gcgtcacttc cggctggtac tgaccacgca ggcgcagcgg gcagaagaag
      661 cccggcagca ggccatttcc ggcgggacgg aaccgtccgc ttttcctgat accctgccgg
      721 gttacaccga atatggccgg gacaacggca tccgtctgtc tgccgtgtgg ctgacgcacg
      781 acccggaata cccggagaat ttacctgccg cgccgctggt gcgctatggc tggacgccgc
      841 gcggcgaact ggcggtggtg tatgaccgta gtggcaaaca ggtgcgcagc tttacttacg
      901 atgataaata ccggggccgg atggtggcgc accgtcacac gggccggccg gaaatccgtt
      961 accgttacga cagcgacggg cgggtgacag aacagctaaa cccggcaggc ttaagctaca
     1021 cgtatcagta tgagaaagac cgcatcacca tcaccgacag cctgaaccgc cgtgaagtcc
     1081 tgcacacgca gggtgaaggc gggctgaagc gggtggtgaa aaaggaacac gcggacggca
     1141 gcgtcacgca gagtcagttt gacgcggtgg gcaggctcag ggcacagacg gatgccgcag
     1201 gcaggacaac agaatacagc ccggatgtgg tgacgggcct catcacgcgc atcaccacgc
     1261 cggatggcag ggcatcggcg ttttactata accaccacag ccagttaacg tcagccaccg
     1321 ggcctgacgg gctggaaata cgccgggaat atgatgaatg gggccgtctg attcaggaaa
     1381 ctgcccctga cggcgatatc acccgctacc gttatgataa tccacacagt gacttaccct
     1441 gcgcaacgga agatgccacc ggcagccgga aaaccatgac gtggagccgt tacggtcagt
     1501 tgctgagctt caccgactgt tccggttatg taacccgtta tgaccatgac cgcttcgggc
     1561 agatgacggc ggtgcaccgc gag
//
LOCUS       DAZFBR010000315         1574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFBR010000315 DAZFBR010000000
VERSION     DAZFBR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.318"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>1574
                     /locus_tag="ACHJZ2_005233"
     CDS             <1..>1574
                     /locus_tag="ACHJZ2_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4571117.1"
                     /translation="TLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQH
                     VPEKEAIVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQVKWDTPRVVKGV
                     RFSLRLTSGSGEGSRLVTTAITADTEHRSSGLPPGEYTLTVRAINSYGQQGEPATTTF
                     RINAPAVPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLG
                     TGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKL
                     HLAQGLWELIDNSQLADEMAEMKTSITETRNEITQTVSKTLEDQSAIIQQIQRVQKDT
                     NDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPEDGNTT
                     PLFVAQGNQLFMNDVFLKRLFAVSITSSANPPTFSLTPEGRLTARNADISGNVNASSG
                     TLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQP
                     FDRQIVIPAVAFSGAK"
BASE COUNT          371 a          413 c          479 g          311 t
ORIGIN      
        1 accctgcctg atggtgtgga gacatacggt gtatggggac tatccctgcc gtcactgcgc
       61 cgtcgcctgt tccgctgtgt ctccgtccgg gaaaacacgg acggcacctt tgccatcacg
      121 gcggtgcagc acgtaccgga aaaagaagcc atcgtggata acggtgcccg ctttgagccg
      181 cagtcaggca ccctgaacag cgtcatccct ccggcagtgc agcacctgac ggtggaggtg
      241 agcgcagctg acggccagta tctggcgcag gtgaaatggg acacgccgcg ggtggtgaag
      301 ggcgtgcgct tcagtctgcg cctgaccagc ggaagcggag aaggcagccg tctggtgacc
      361 accgccatca ctgcggatac agagcatcgt tccagtggtc tgccgccggg ggaatacacc
      421 ctgacggtca gggcgattaa cagttatggc cagcaggggg aaccggccac caccacgttc
      481 aggattaatg cacctgcggt acccgccacg attgagctga caccgggcta ttttcagata
      541 acagcggtcc cgcgtcttgc ggtgtatgac ccgacggtac agtttgagtt ctggttttcg
      601 gagacaaaaa tcgcagatac atctcaggtg gaaacctctg cccgttatct ggggaccggc
      661 agtcagtgga gtgtatccgg cccgcacatt aagcccggga aggatttctg gttttacgtg
      721 cgcagcgtca acctggtggg gaaatctgct tttgtggaag ccagtggccg ggccagcaat
      781 gatgcagaag ggtatctggg gctgtttcgg gaaaaaatag gaaaactgca tctggctcag
      841 gggctgtggg agctgataga caacagccag cttgcggatg agatggcgga gatgaagacc
      901 tccatcaccg aaacccgcaa tgaaatcaca cagacggtca gtaaaacgct ggaggaccag
      961 agcgccatca tacagcagat acagcgcgtg cagaaggaca caaatgatga ccttgctgca
     1021 ctttacatgc tgaaggtaca gaaaacaaaa aatggcatac cctatgttgc cggtattgga
     1081 gcggggattg aggatactga tggccagccc ctgagcaaca tactgctgct ggctgaccgt
     1141 attgcgatga ttaacccgga ggacggcaac accacgccgt tatttgtggc gcaggggaat
     1201 cagttgttca tgaacgatgt gttcctgaaa cgactgtttg cggtgagtat cacgtcatcc
     1261 gccaatcccc cgacgttttc cctgacgccg gagggcaggc tgaccgcaag aaatgctgat
     1321 atcagcggta acgtgaatgc gagttccggg acgctcaaca acgtcacgat taacgagaac
     1381 tgtcgggttc tgggaaaact gtccgcgaac cagattgaag gcgatctcgt taaaacagtg
     1441 ggcaaagctt tcccccggga ttcccgtgca ccggagcggt ggccatcagg gaccattacc
     1501 gtcagggttt atgacgatca gccgtttgac cggcagattg ttattccggc ggtggcattc
     1561 agcggcgcta aaca
//
LOCUS       DAZFBR010000316         1569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFBR010000316 DAZFBR010000000
VERSION     DAZFBR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.319"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..687)
                     /locus_tag="ACHJZ2_005234"
     CDS             complement(<1..687)
                     /locus_tag="ACHJZ2_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571118.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNININCPLKDIYNEFRRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNPFKLNSTNFTNANMFQVNFVSSTQNAKISWDYLLKITPV
                     LTSISDMYSEE"
     gene            complement(709..1248)
                     /gene="yhaB"
                     /locus_tag="ACHJZ2_005235"
     CDS             complement(709..1248)
                     /gene="yhaB"
                     /locus_tag="ACHJZ2_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO4571119.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEKCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAYFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1504..>1569)
                     /locus_tag="ACHJZ2_005236"
     CDS             complement(1504..>1569)
                     /locus_tag="ACHJZ2_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO4571120.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          494 a          265 c          282 g          528 t
ORIGIN      
        1 ttcttcagaa tacatatcgc taatgcttgt taaaacaggc gttattttta gtaaataatc
       61 ccaggagatt ttggcgtttt gtgttgaact aacaaaatta acctgaaaca tgttggcatt
      121 agtaaaattt gttgaattca atttaaaagg atttaaaata gtattagcca tatgtttatc
      181 tgtgaaatca taataatcta aatcgacatt acgtaagtcc agagaggata gatcaacgat
      241 atctccagtc ccacaactaa aaattacgtt taacctcctg aattcattgt aaatatcttt
      301 taatgggcaa ttaatattga tattacctgt ggatatttcc tggtttttta acggttcatt
      361 aatggtaagt tcacaagtat tgtccgatcg ttgaataatt tgggcgttat aacctaaaaa
      421 atccagacga agagacgagt cattataaag aattacattt tgtaagtacg ctaattcttt
      481 ttgaaaatca ccagtgataa tgcattttcc actaggttgt aacgtgttaa gtaaagtgac
      541 catttcaata gaggtctgtt ttagtttttc aatacctggt gctagtgctt cgaggttatt
      601 tcctgttggg ctttcaggta ggtcattcat ttttcgacgg actaaatcac tactaatatc
      661 attaccaatt gatgaaacag ggaacattat tttatccttt ataaataatc aataaaaatc
      721 attattaaaa tgatcgggtg aaaaaaagag aatttgccaa caatctgaag taatactggt
      781 tttattaaag tacgcagggg gtgttaagat ggcttctttt cgggtgaatt ttgcacaata
      841 cttagaaatc gtctccttaa tacattccag ctctttttta ttgacggctg agcaagtaca
      901 tttttcgcca aaaggtgcaa aagtgcctac atctaatgtt tgtatgatat aatcgtcatg
      961 ttctcctgta gaaatatcta tgatgaatgg actcatattt tccggttgga ttcggatcat
     1021 gacagaagga acatcgcctt gatagaaatt caatgcagta attgcagata atactctccg
     1081 cagttcatta cttatactgt aaattttatt aacgtcattg gtggagacaa tttctgcaaa
     1141 ctttcttttt accgtccaat aatcaatatt tctattgtga ttgttaacca ctgcgtgcat
     1201 tggtgggatt gaagggtgaa aaatatgtgc aattgggaag cctttcattg atgcctgcct
     1261 tgtcatcatg acaaacaata atagatgctt attttcctcg cagggttctt tcgcactatc
     1321 aaaaaacgct catttacccg gcgttacagt aaacgaagca acattctgtg gcctacgccg
     1381 atcaaaagct ctgtgaaaat ttcccttgtt gtctttgtct gaccttatcg cggcgatcag
     1441 caattattcg ttttgtgatc ctcaacacaa aatgctattc gtcgcctatg aaggaagaag
     1501 aatttatgga aaacgattgc attcacaaaa tgacgtcctg tcagaagaga aaaagttgac
     1561 gttaatcaa
//
LOCUS       DAZFBR010000317         1554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFBR010000317 DAZFBR010000000
VERSION     DAZFBR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.320"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..198
                     /locus_tag="ACHJZ2_005237"
     CDS             <1..198
                     /locus_tag="ACHJZ2_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4571121.1"
                     /translation="MLANTCMWDYRGDECGYHGPAVADEFDNPTTDIRKDRCSKCMRG
                     CEMRGMVANFGGFLSINKLSQ"
     gene            complement(215..469)
                     /locus_tag="ACHJZ2_005238"
     CDS             complement(215..469)
                     /locus_tag="ACHJZ2_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4571122.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            579..689
                     /locus_tag="ACHJZ2_005239"
     CDS             579..689
                     /locus_tag="ACHJZ2_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4571123.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVM"
     gene            992..>1554
                     /locus_tag="ACHJZ2_005240"
     CDS             992..>1554
                     /locus_tag="ACHJZ2_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO4571124.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEF"
BASE COUNT          452 a          294 c          384 g          424 t
ORIGIN      
        1 atgctggcga acacctgtat gtgggattac cggggagatg aatgcggtta tcacggtcct
       61 gcggtggcgg atgagttcga caaccccacc acggatatcc gtaaggacag atgcagcaag
      121 tgcatgcgcg ggtgtgagat gcgcggcatg gtggctaatt ttggcggttt cctttccatt
      181 aataaacttt cgcagtaatg gattatgccc accatcaggt gggtttttta tcttcgccgt
      241 acatgctgct gagagtctta agaagagcct ccctaaattt ttcagattct tgttgagcaa
      301 aaccttctat tgatcttggt gatttttctt catcaatagc agcttgcaag atcataacta
      361 tttctgaatt taatgagcgt ccgtttttcg atgctcttat ggtcaggtcg cgcttgaggc
      421 tatctggcat tcttacgcta tatggggcta tgtctctaac cttcgtcata cttacaccgt
      481 gataatcaca ttgatatcac agtgtattca aaaaaacttt gacttgatat agtcacagtg
      541 atacgtttgt gattcataat aactcacgcg gtggcgatat ggaaaaagaa gttagcagaa
      601 ttttggtgag aatacctcag tcgctaaaag atgcgattac aggaaaggcc aaagaagaat
      661 gccggtcgtt taactcggaa gtgatgtgat tgctggacag cctgaagaga gaggggataa
      721 cggtatgagt aaagaatgtt gttttttgcg gcattagcga atcagacgct gatcaaacat
      781 acatttactc taaagaaaca ggtcggatgc tgtgtagtga ctgcgtgttt gacatcataa
      841 gatacaagta tcttggatgt tctgccagca ttagcaatat aggtgaagtg tacgaaggga
      901 aagatataac tgatagagca gaaagttgaa gccccaactg ctgtaacagt cagggcttcg
      961 ttatcaacaa atcggcttag gaaatattga catgaaaagt atagcaaagg cacaaaacga
     1021 tttcactatc ttcaaattcg gcgacagtga aatccgcgtc atcaacaagt gcggtgagcc
     1081 gtggtttgta gctaaagatg tttgtgatgc tttagctttg actaactcac gcaaggcgct
     1141 tactgcactt gatgacgatg aaaagggagt aactttaagt tacacccttg gtggtgagca
     1201 gaatctaagc attgtgagcg aatcaggtat gtatacattg gttctgcgct gccgcgatgc
     1261 agtcaataaa ggttcagtcc cgcacaaatt ccgcaagtgg gtaacagcag aagttctgcc
     1321 ttcaattcgc aaacatggcg agtatgtaaa aggaaagaaa accactgttg aggaaagaac
     1381 accgctacgc gatgcagtaa acatgctggt aggaaagaaa ggacttcgca atgacgatgc
     1441 atacaatatg gttcatcagc gttttggtat tgacagcatt gatgagcttt caattgaaca
     1501 aatcccgctt gccgtagagt acatccacag ggtagtgctt gaaggtgaat tcat
//
LOCUS       DAZFBR010000318         1540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFBR010000318 DAZFBR010000000
VERSION     DAZFBR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.321"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(244..1119)
                     /locus_tag="ACHJZ2_005241"
     CDS             complement(244..1119)
                     /locus_tag="ACHJZ2_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO4571125.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTLGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
     gene            complement(1260..1529)
                     /locus_tag="ACHJZ2_005242"
     CDS             complement(1260..1529)
                     /locus_tag="ACHJZ2_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4571126.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
BASE COUNT          407 a          344 c          295 g          494 t
ORIGIN      
        1 gaaacagcat tgtaaataaa atgtaataat tacatactat tagtgattct catgcatcgt
       61 aagcggctcg ccagaaccgt attgatattt actgagcgct cagatcaact ttccatggca
      121 acagatcgcg tacccggttt gccggccagt cctggatatg ctcccgccga caatattaat
      181 ggccaggaaa tgtttaaaac aatgattctg tttcctaacc atcaagtgta tatgctggct
      241 caattactca atatctatgg gttgagttct acccaaagtg gctctgtagt cgcttcttct
      301 tacagtatgt tcaggtgttg caggcacagt tgtggagcgt agatgcgttg ttggtttacc
      361 atgctctggt acataaaaaa cgccagtgcc atggtaacct gatatagaaa cgttaattcc
      421 gcgacgttca aactcggcat agacgtgttc tgctaaagac ctcttttgac caaataatgc
      481 cctggccaac cagccgttat tcatatttgc ggatttttct atttcatcag gagagaagtc
      541 tttgtttttt attatgttgg ctgagttaca ggatgttaat ctgatatcat ctatttcatg
      601 aagattatgc tgaacaatgc ggtcgacaat atctgacggt gataaaaatt catctccgca
      661 cttaagaaga ggtagaccag cagaaccatg cccagacaga taaattttgt tgtattttcc
      721 tagtgttaaa tcatctggca ggatggtttt catctctgtc gctgtaatac ttatagctgc
      781 agcagcaaca acatcgctat tacttgattg tagatggctt ttatttgctc caggatatgt
      841 aaactccatt ttccttttat caaaatcttg ttttgtagca tcattcagga ataataaatc
      901 atacggtttt ttccccgttg tgactcttga aacattttgg ccaagaatat taagagtata
      961 gtcattaacg gatttcattc ctaaaaaaac aagtaatcct tctttctctt gcgctttttc
     1021 gattgttctc cctgctaaag ttattgggat ggattgagat tgttttgtcg gaactgatac
     1081 gctggcgcta ataggtagta gggttcttat gctaaacata caacctcttt cttaattttc
     1141 gaacctgttt aggattctgt gtaaattcaa aataaacctg tctgaacatt cctaacaaac
     1201 atccaccgga catgacaaca aaaaccggag ccggactccg gtttttgtga agctgtcggc
     1261 tatttcattc cgccaatatt ttcccacctc ccgtcagcac gcagaatttg cagcggtctt
     1321 accacgcact gtatctgctt tttatccgca tccagtatca ccacctgcgt gattaccctg
     1381 tcctgctccg gaataatacc attctcatcg gactccagga tgtctgccgg ccccagtcgc
     1441 agttgtgctg taagcgactg cacgtgttca cggccatcat gctttccgca accacacaga
     1501 cgctgcataa gtttttttag tatattcata tcattctcct
//
LOCUS       DAZFBR010000319         1536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFBR010000319 DAZFBR010000000
VERSION     DAZFBR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.322"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..230)
                     /locus_tag="ACHJZ2_005243"
     CDS             complement(<1..230)
                     /locus_tag="ACHJZ2_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571127.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAVHQPLAEDKSLLPFFSDERVIRAAG"
     gene            complement(232..510)
                     /locus_tag="ACHJZ2_005244"
     CDS             complement(232..510)
                     /locus_tag="ACHJZ2_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571128.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(678..833)
                     /gene="hokD"
                     /locus_tag="ACHJZ2_005245"
     CDS             complement(678..833)
                     /gene="hokD"
                     /locus_tag="ACHJZ2_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4571129.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVF
                     TAYEPEE"
     gene            1438..>1536
                     /locus_tag="ACHJZ2_005246"
     CDS             1438..>1536
                     /locus_tag="ACHJZ2_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4571130.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNI"
BASE COUNT          409 a          362 c          369 g          396 t
ORIGIN      
        1 cccgcagcac ggatcacccg ctcatcgctg aaaaatggca gtagtgattt atcttccgcc
       61 agcggctggt gaacggcagg tacaaccccg gacggcagag ctcgcatgct ttttggttcc
      121 ggctccacca gtattctgcc gttatggaat gctgacattg attcacggcc tggcttaacg
      181 ataaccagac cgagttccgg taccagaaca ggtcgaagta atacccgcac attacctcca
      241 gatccgttgc tggaatgtgc gggacggacg cggtggccgt tcggagtaag ggagcctgac
      301 ggagattatc cagtgacggt agtcgaggct aagggctttt ttaacctcgc atccgcgcct
      361 gcggtaacac tgaatgagcc attcggcctg ttcttcagtg catgggggat gctggtacca
      421 gtcagatttg aatgcgtgaa aacaccgtcc gcgcctgctg gcaaagacgg cagaatcatc
      481 agaattgtat aatttggtat cgtgcgccat cggttgtctc tgctggcgca gcaggtgcca
      541 gttgttcagg ctggcgtgcg aattgtaaac cagaatgcca ggaaaaaaca aaacccgccg
      601 aagcgggtta agtgcgggtg cgttgaggat gcctgattca tcagaggtgg cgagggattt
      661 ctcccccgcc aggtctctta ctcctcaggt tcgtaagctg tgaagacagc gacctccgtc
      721 tggccggttc ggactcgtac ctcgcagagg tctttcctcg ttaccagtgc cgtcactatg
      781 acggttaaac agatgacgat cagggcgatt aacatcgcct tttgctgctt catagcctgc
      841 ttctccttgc ctttcggcac gtaagaggct aacctagatt tgccgttcat agattgagcc
      901 tcagattaat gttaggcgtc ttgcaggacg cgtaatgtta actggggctt ttctctatct
      961 gccgttggtg ttcatgcccg aggcagatag cctcaagcac ccgcagctat tctacttaac
     1021 ttcccattac ctcgccaata tgaaatcggt cagaaagaca gacgtcccat aaggacaaca
     1081 acggaaaaag agattttgct tgatgctatt tacctctgca ccatgaagtt gcgccaaaat
     1141 cccagtggtg ataacaggaa tttggttgtt ggtgattggg gaaagaagta cgttagactg
     1201 tgggttagtt cattgacatg atagttacct gccttcaatt tcctaagcaa gcgatgtaaa
     1261 cgataatcaa tatcaaatgt attgacaggt aaaataataa cgatgtacct ttgctgataa
     1321 catatatcag tcttaaatca taatggaggt agataaaata actaaacaca cacaacaaat
     1381 tttttatcag tgacatagca tatcttaagc atcctttaat gctcatgggg agaacgcatg
     1441 actaaacata tggacattca ttgtcaaaca cgcatattta actcaattaa ccatgacacc
     1501 aaatataaaa tatgtatttt tactccaaaa aacatt
//
LOCUS       DAZFBR010000320         1535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFBR010000320 DAZFBR010000000
VERSION     DAZFBR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.323"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>1535)
                     /locus_tag="ACHJZ2_005247"
     CDS             complement(<1..>1535)
                     /locus_tag="ACHJZ2_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4571131.1"
                     /translation="DNVIKFIEFCKSTISNNNLTTSWESNKWKGLYRFTKFNSKNNLN
                     SKECLDDSFINFAKAYMLHVHSFNKSKTKHSTLSMLKIVEFVLLKINMEANVNYCNNS
                     IYDECIRIASEKYSKAHAFAIGKELEKLSSFLNDNRMTNSFYLFWVNPIRYRITQSWT
                     GYDSSLEGHSRLPDIKSVIAIAEIFSKRDEQLSSRDIFTTSVLALLMCAPSRISEILA
                     LPADCEITECDGKGIQRYGLRFFSAKGYEGNIKWIPTLMIPVAKKAISRLKELSSQAR
                     LLAAEIQKNYSNSTKGTLKENIPPDLFWYDREKKIKYSNALCLLTEGQLNQNKKEMSD
                     KLFRPTTNFFKTDIIDSDYIKGYFNVFKRHGYINEDGSPYLLRTHQLRHLLNTFAQIN
                     GMDEFSIARWSGRKLISQNVSYDHRSHLQMSKAIREKKLSVCVNEHRIKDIPVVDLNE
                     FDSLSSGAVLVSKHGYCKHSYAFKPCDNYPIKNSGLDNETISNIHDKILKRTLYDKND
                     GNI"
BASE COUNT          490 a          277 c          214 g          554 t
ORIGIN      
        1 ttatgttccc atcattttta tcatacagtg ttctttttaa aattttatcg tggatatttg
       61 aaatcgtttc gttatctaaa ccagagttct taattggata attatcacac ggcttaaacg
      121 catatgagtg cttgcagtag ccatgttttg atacaagtac tgcaccacta ctaagtgagt
      181 caaactcatt aagatccact actggaatat cctttattct gtgctcatta acacatactg
      241 ataacttttt ttctcttatt gctttagaca tttgaagatg cgatctgtgg tcataagaaa
      301 cattttgaga aataagctta cgtccagacc agcgagcaat actaaattca tccataccat
      361 ttatttgagc aaatgtgttg agaagatgcc ttagttgatg tgttcttagc aaatatgggc
      421 taccatcttc atttatataa ccatgtcttt taaaaacatt aaaataccct tttatataat
      481 cagaatcaat gatatcagtt ttaaaaaaat tcgttgtagg tctgaataat ttatctgaca
      541 tttccttttt attttgattt aactgtcctt cagttaacaa gcaaagcgca ttagaatatt
      601 tgattttctt ctctctatca taccaaaaga gatcaggagg tatattttct ttaagggttc
      661 ccttcgttga attagagtaa ttcttttgaa tttcagcagc caataacctc gcttgacttg
      721 ataattcttt taatctgcta atagcctttt tagctacagg tatcattaaa gttggaatcc
      781 attttatatt gccttcatac ccttttgctg aaaaaaatct taaaccgtat ctttgaatgc
      841 cctttccatc acattctgtg atttcacaat ccgctggcaa agctaaaatt tccgatatcc
      901 tactcggtgc acacataagt aaagcaagca cagatgtagt aaatatatct cttgacgata
      961 attgttcatc ccgctttgaa aaaatctcag caatcgcaat aactgatttg atatcaggca
     1021 atctagaatg gccttccagt gaagaatcat aaccagtcca agactgagta atcctatacc
     1081 taattggatt tacccaaaat aaataaaatg agttagtcat cctattatca tttaaaaatg
     1141 aactcaattt ctcaagttct ttcccaatag caaatgcatg tgctttagaa tacttttcag
     1201 aggctatcct tatgcattca tcatagattg aattgttgca atagtttaca ttagcttcca
     1261 tattgatttt aagtaaaacg aattcgacaa tcttcaacat tgataatgtt gagtgtttcg
     1321 tcttagattt attgaatgaa tgcacatgca acatatatgc tttagcaaaa ttaataaagc
     1381 tatcatctaa gcactccttg ctgtttaggt tgttttttga gttaaactta gtaaacctat
     1441 ataaaccttt ccatttattg ctttcccatg aagttgttaa attattatta gaaatagtac
     1501 tcttacaaaa ctcgataaac ttaataacat tatcg
//
LOCUS       DAZFBR010000321         1529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFBR010000321 DAZFBR010000000
VERSION     DAZFBR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.324"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            153..1478
                     /gene="nleA"
                     /locus_tag="ACHJZ2_005248"
     CDS             153..1478
                     /gene="nleA"
                     /locus_tag="ACHJZ2_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO4571132.1"
                     /translation="MNIQPTIQSGITSQNNQHHQTEQIPSTQIPQSELPLGCQAGFVV
                     NIPDDIQQHAPECGETTALLSLIKDKGLLSGLDEYIAPHLEEGSIGKKTLDMFGLFNV
                     TQMALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTAVSTIASTGTSATTSTVTTEPKDPIPWFGLTAQVVRNHGVELPIVK
                     TENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDL
                     DNPNTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPG
                     LKPDTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGAD
                     IRAMEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDIFDIIQETRV"
BASE COUNT          504 a          304 c          307 g          414 t
ORIGIN      
        1 gtaaatttaa caacttaatt ttgagatcta agtcacatca atcaagcatt caaacagtta
       61 tatattaaag ctgtccacat cggatatgtg acactaatag tatcaatgga ttgatattat
      121 taatggatat aaacatgtaa taaggattta ttatgaacat tcaaccgacc atacaatctg
      181 gaatcacctc acaaaacaat caacatcatc aaacagaaca aataccctct acacaaatac
      241 cgcaatccga attacctcta ggatgccaag ctggatttgt tgttaatatt ccagatgata
      301 tacagcaaca tgcaccggaa tgcggtgaaa caacagctct actgagcttg ataaaagata
      361 aaggtctgct ctcagggcta gacgaatata tagctcctca ccttgaagaa ggatccatag
      421 gaaaaaaaac attggatatg tttggtttat tcaatgttac ccaaatggca ttagagatac
      481 ctagttccgt ttcaggcatc tctggtaaat atggtgtcca gctaaacatt gtaaaaccag
      541 atattcatcc tacatcaggt aattattttt tacagatatt ccctctgcat gatgaaatag
      601 gttttaattt taaagacctt cctggcccgt taaaaaatgc attaagcaac agtaatatat
      661 caaccactgc agtgtcgact attgcatcga ctggaacatc agccactact tcgacggtaa
      721 ccaccgagcc aaaagaccca ataccatggt ttggattaac agctcaagtg gttcgtaatc
      781 atggtgtaga acttcctata gtcaaaactg aaaatggatg gaagcttgtt ggagaaacac
      841 cacttactcc tgatgggccg aaagcaaatt acacggagga gtgggttatc agaccgggag
      901 aagcagattt taaatatggt gcatctccat tacaggcaac tctagggctg gagtttggcg
      961 cacatttcaa gtgggattta gataacccta atactaaata tgccgttctt accaatgctg
     1021 ccgcaaatgc gcttggtgct ttagggggat ttgcagtatc cagatttgct agtacagatc
     1081 caatgttaag tcctcatatc ggtgcaatgg ttgggcaagc agcagggcat gccatacagt
     1141 ataatacccc tggattaaag ccagacacta ttttatggtg ggctggtgcg acactggggg
     1201 ctgccgattt aaacaaggcc gagtttgaag tagctagatt cactgactat cctcgtatat
     1261 ggtggcacgc aagagaagga gctattttcc ccaataaagc agatattgaa catgccacag
     1321 gtgctgatat acgcgcaatg gaagaaggta tccctgttgg acagcggcat ccaaatccag
     1381 aggatgtggt aatcgatatc gaaagcaatg gcttaccaca tcataatcca tcaaatcatg
     1441 ttgatatctt tgatataatc caagaaacaa gagtctaagc tttaacttgt ttattttaaa
     1501 ataatagctg aaatggatgg cagctatta
//
LOCUS       DAZFBR010000322         1493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFBR010000322 DAZFBR010000000
VERSION     DAZFBR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.325"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1322
                     /gene="espX1"
                     /locus_tag="ACHJZ2_005249"
     CDS             <1..1322
                     /gene="espX1"
                     /locus_tag="ACHJZ2_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO4571133.1"
                     /translation="FCEYKTEFLILFDDFFRSQDLPKPSPVLHHFFQYTHLRDAHFYR
                     CKLIEHTVQFSFFKHKGITLLRLDVFDDRTSECLSEEIKIYQECHEKFIKFLKANFNQ
                     EIYPELYTPEIFYEACRNLQSFYDHQETSQNAKYSAIVKKKSYFNKEIRNLIKKNIYP
                     ELYNEQCNKIPASSTDDNQKITWQNFKTSNAAYSQLCEKLSLLKSSPSRLIEKSAYCS
                     NENMITDKFDVVFSYCGDNVKEFILLLPYNKSLEMHELNEQNIQYLTALNINIHKLLL
                     SNITIEKSNLSYGYYFGCVLSNISCFESDLSNTIFSNGEINNLFIKKSNIFGTSFTNT
                     MIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRD
                     NVKLNNDVYKELKKFMHDYDKTSPEMRKYLQSINESMFLMKKISHQD"
BASE COUNT          567 a          259 c          224 g          443 t
ORIGIN      
        1 tgttttgcga gtataaaaca gagttcttaa ttttgtttga tgactttttc agaagccagg
       61 atttgcctaa accatctcca gtcctccatc atttttttca atatactcat ctgagagatg
      121 cacattttta tagatgtaaa ctcatagaac atacagttca attttctttt tttaaacaca
      181 aaggcatcac attacttaga ttggatgttt ttgatgacag aacaagcgaa tgtctttctg
      241 aagagattaa gatatatcaa gaatgccatg aaaaatttat taaattttta aaggcaaact
      301 tcaatcagga aatataccca gagctataca ctcctgaaat attttatgag gcctgcagaa
      361 atctccagtc attttatgat catcaggaga cgtcacaaaa cgctaagtac agtgcaatag
      421 tcaagaaaaa aagttatttt aataaggaaa tcagaaatct aataaaaaag aatatttatc
      481 ctgagctata caatgagcaa tgcaataaaa tccccgcctc atcgacagat gataaccaga
      541 aaataacatg gcagaatttt aaaactagca atgcggcata ttcacaacta tgtgaaaaat
      601 tatctttatt aaaaagctcg cccagtcggt taatagagaa atcagcttac tgctcaaatg
      661 aaaacatgat caccgataaa tttgatgttg tcttttctta ctgcggagat aatgtaaagg
      721 aatttatact tttattaccc tataataaat cgctggagat gcatgaactt aacgagcaaa
      781 acatccagta tttgactgcc cttaatatta atatacataa actgttgcta tcaaatataa
      841 caatagaaaa gagcaacctt tcatatgggt attattttgg ttgtgtatta tccaatatat
      901 catgttttga aagcgactta tcaaacacca tcttttcaaa tggagaaata aacaacctct
      961 tcattaaaaa aagcaacata tttggaacat cgttcactaa taccatgata aaaaatttgc
     1021 gctgtgaaga tattatgcct ggccgatgga ccactcaact tgtcaataag cacttaggtt
     1081 acagatacac aggagtattc aaaaccctgg caagtattga tgacaaaccg tcccgtttcg
     1141 aaattttgat accattggta caaactttag ttcgcgacaa tgtaaagcta aataatgatg
     1201 tgtataaaga actgaaaaag tttatgcacg attacgacaa aacatcccca gaaatgagaa
     1261 aataccttca gagcatcaac gagtctatgt tccttatgaa aaaaatatct catcaagatt
     1321 aaaacacccc aaaaaagaag taatttcaga atgaaattac ctgtgataat tattaattaa
     1381 ctgcagagaa ataaaaaata atctgaagca tgctctgaaa catcctactc tgtcactcta
     1441 atgatggcac cttgctcttc ttataaagaa gggaataatt aagaatatta aat
//
LOCUS       DAZFBR010000323         1485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFBR010000323 DAZFBR010000000
VERSION     DAZFBR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.326"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(18..>1485)
                     /locus_tag="ACHJZ2_005250"
     CDS             complement(18..>1485)
                     /locus_tag="ACHJZ2_005250"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO4571134.1"
                     /translation="VFLISALNFVFTPFSFRYIFKPFFCILFICSSIVTYATMKYGVQ
                     FDKTMMQNIFETNAGEMTSYFNMSVVLWFLFTGILPCGLLLLVNIRYPETWIKGITYR
                     LISMFASLLIIFAIAFFFYKDYASVGRNNSSLNKEIIPTNYIYSGFKYVRDFFVSPGE
                     FRQTGTDASRTINEKQKPVIMFLVVGETARSQNYALNGYSRGTNDFTKKYNELISFHN
                     VQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVNLLWIENDGGCKGV
                     CKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYINNNSEDKLIVFHLMGSHGPTY
                     YLRYPESHKYFKPTCDRSDIENCTHEQLINTYDNTIRYTDYIISKLIDKLIEYKDEYD
                     TVLLYVSDHGESLGENGLYLHGTPYNVAPAEQTHVPLITWMSPGFVSSKKIDLNCLSE
                     HALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR"
BASE COUNT          512 a          269 c          226 g          478 t
ORIGIN      
        1 cagaaaaatg tcagtatcta tctgcaggag gatataatat catcttcttt attataaact
       61 gaagtattta cattccataa ccccagtaac gatgaaaata tattatcatg acttactgtt
      121 ctgtttagag catgttctga caaacaattc aaatcaattt ttttagagga aacgaacccc
      181 ggagacatcc aggtaataag aggcacatgc gtttgctcag ctggagcaac attatatggt
      241 gttccatgta agtataatcc attttctccc agagattctc catggtcaga gacatataat
      301 aaaacagtat catactcatc tttatattcg ataagcttgt caattaattt tgatattata
      361 taatcagtat agcgaattgt attatcatag gtattaatca attgctcatg cgtacaattt
      421 tcgatatcac tacgatcaca tgttggttta aaatatttat gtgactcagg ataacggaga
      481 tagtaggtag gaccatgact acccatcaaa tgaaatacga taagtttatc ctctgagtta
      541 ttatttatat actcatcaat attttttagc ataacctcat cataacatga gtttttcttg
      601 cataatgtat tatctgaatt tgatggctca atattaattg tagggattcg cttacataca
      661 cctttacatc ccccgtcatt ttcaatccag agtaaattta cccccgttct gtacagaata
      721 tccagtacat tttccgaatt taccgctttg cgggaattga actcttttcg cttcatatct
      781 gaaaacatgc atggaacaga tatggctgtg gatgtgccac acgactgcac attatgaaag
      841 gatataagtt cattatattt ctttgtaaaa tcattggtac cacgagaata tccattcaga
      901 gcatagtttt gacttcttgc tgtctccccg acaactaaaa acataattac cggcttttgt
      961 ttttcattta ttgtgcggga tgcatccgtt ccagtctgac gaaactcgcc aggagaaaca
     1021 aaaaaatccc ttacatattt aaacccactg tagatataat tagtaggtat tatttcttta
     1081 tttaatgaag agttgtttct gccaactgat gcataatcct tataaaagaa aaacgcaatc
     1141 gcaaatataa tcaagagaga tgcaaacatt gatattaaac gatatgtaat accctttatc
     1201 catgtctcag gataacggat atttactaac aacaatagtc cacatggaag tatacccgta
     1261 aataaaaacc acagtacaac agacatattg aaatatgatg tcatttcacc tgcatttgtt
     1321 tcaaaaatat tctgcatcat tgttttatca aactgcacac catatttcat ggtggcatat
     1381 gtaacaatgg aactacagat aaacagaata caaaaaaaag gtttaaatat atacctgaat
     1441 gagaacggtg taaaaacaaa atttaatgca gatatcagaa agact
//
LOCUS       DAZFBR010000324         1474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFBR010000324 DAZFBR010000000
VERSION     DAZFBR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.327"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..357
                     /locus_tag="ACHJZ2_005251"
     CDS             <1..357
                     /locus_tag="ACHJZ2_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4571135.1"
                     /translation="KSEQEDKPRRREAAAIPQPDETNPEMPPPVVISPGIEYMEDGLP
                     DPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGDAAKKAANRFAV
                     PPPPRLVVNNDNEGNG"
     gene            354..>1474
                     /locus_tag="ACHJZ2_005252"
     CDS             354..>1474
                     /locus_tag="ACHJZ2_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4571136.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDR"
BASE COUNT          326 a          374 c          473 g          301 t
ORIGIN      
        1 aaatcagagc aggaggataa accccgtcgc cgtgaggcgg ctgcaatacc acagccggac
       61 gaaacaaatc cggagatgcc accgcccgtg gtgatatctc ctggtattga gtatatggag
      121 gacggtcttc ccgatccggt gaaagcgatg gggcgtcttc tggtggagaa cattaatacc
      181 gaccccaggc tggcgctgga tgcggcttat aagctggcgc agttcacgca ccacaaaaaa
      241 ggggatgccg gtaaaaaatc ggcaaaaggt gacgcggcga aaaaagcggc taaccgtttt
      301 gcggtgccac caccaccccg cctggtggtg aataatgata atgagggcaa cggatgatac
      361 ctgtgtggag cacggcctgc ccggactggg cagagcgcct gaaaaagggg ctgtcgatta
      421 ttccggctcc gatttatccg gagcaggccg cacatgccct ggcgattttt aaacaactgc
      481 ggattgtgga tgcaccgggc agcccgacgt tcggggagtc ctgcgcacag tgggtgtttg
      541 acctggtggc ggccctgttt ggctcctacg atgcgcagac cggtgtacgc catatcaagg
      601 aagtttttat ccttatcccc aagaaaaaca gcaagtccac gctggctgcc gggatcatga
      661 tgacggcgct gttactgaac tggcggcagg cggcgggcta caccattctg gccccgaccg
      721 tggaggtggc ggctaacgcc ttcaaccctg ccagggatat ggtacgacgg gacgatgatc
      781 tggatgacct ctgtcaggtg cagacacata tccggaccat cacccacagg gtgacggaca
      841 ccaccctgaa ggtggtggct gccgatccga atacggtatc cggtatcaag tccgtgggga
      901 cgctgattga tgaactgtgg ttatttggca agcagtacaa agcggaggac atgttacgtg
      961 aagccatagg cggccttgcc tcccgcccgg aagggtttgt ggtgtatacg accacccagt
     1021 cgaatgagcc gccagccggg gtgttcagac agaaactgca gtacgcccgg gatgtccgtg
     1081 acggcaaaat tcatgatccg cactttctgc cggtgatttt tgagcatcct cctgaaatgg
     1141 tggaaagcgg ggctcacctg ctgatggaaa acctcgccat ggttaacccg aatctcggtt
     1201 attcggtgga tgaggctttt ctgtaccggg agtaccgtaa agcccgggag gctggtgagg
     1261 aagcatttcg tggcttcatg tcaaaacatg ccaatgtgga aattggtctt gccctgcgtt
     1321 ctgaccgctg ggcgggtgcg gatttctggg agcagcaggg caggcgcgtc agcctggacg
     1381 atatcctgca gcgcgctgat gtggtgacgg tggggattga cggcgggggc ctggatgatc
     1441 tgctgggaat gtacgtgatt ggccgtgaca ggga
//
LOCUS       DAZFBR010000325         1454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFBR010000325 DAZFBR010000000
VERSION     DAZFBR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.328"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..193
                     /locus_tag="ACHJZ2_005253"
     CDS             <1..193
                     /locus_tag="ACHJZ2_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4571137.1"
                     /translation="TNSYGWFPGTDGALITGLTFLDPKDATQVQGMFQHLQVRFGDGP
                     WQDVKGLDEVGSDTGRTGE"
     gene            195..464
                     /locus_tag="ACHJZ2_005254"
     CDS             195..464
                     /locus_tag="ACHJZ2_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4571138.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGRSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            466..573
                     /locus_tag="ACHJZ2_005255"
                     /pseudo
     CDS             466..573
                     /locus_tag="ACHJZ2_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            641..>1454
                     /gene="nleB2"
                     /locus_tag="ACHJZ2_005256"
     CDS             641..>1454
                     /gene="nleB2"
                     /locus_tag="ACHJZ2_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFO4571139.1"
                     /translation="MLSPIRTTFHNSVNIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYREDVDFSMYDRKLSDIYHDIICEQRLRTEDKRDEYLLNLLEKELR
                     EISKAQDSLISMYAKKRNHAWFDFFRNLALLKAGEIFRCTYNTKNHGISFGEGCIYLD
                     MDMILTGKLGTIYAPDGISMHVDRRNDSVNIENSAIIVNRSNHPALLEGLSFM"
BASE COUNT          460 a          225 c          366 g          403 t
ORIGIN      
        1 gacaaacagc tatggctggt tcccgggtac agatggtgcg ctcatcaccg gactgacctt
       61 tcttgacccc aaagatgcca cacaggttca ggggatgttt cagcatttgc aggtcagatt
      121 tggtgacggg ccatggcagg atgttaaggg actggatgaa gtgggcagtg atacaggcag
      181 aactggagaa tgacatgaat attttgagaa agcttatgca gagtctgtgc ggttgcggaa
      241 agcatgatga ctgtgaaaac ggacggtcac ttacagcaca attgcgactg ggaccggcag
      301 acattctgga gtcagatgag aatggcatta ttccggagca ggacagggta atcacacagg
      361 tggtgatact ggatgcagat aaaaagcaga tacagtgtgt ggtaagaccg ctgcaaatcc
      421 tgcgtgctga cgggacgtgg gaaaatattg gcgggatgaa gtaacccgac agcttcacaa
      481 aaccggagtc cggctccggt ttttgttgtc atgtccggtg gatgtttgtt aggaaagcaa
      541 agatggcaaa actgctggag gttttgtggt tgagtatgcc aatataatta atagattaaa
      601 gagttagttg tgaagaaaat atggataaac aggacgacga atgctttcac cgataaggac
      661 aactttccat aactcagtaa atatagtgca gagttcaccc tgtcaaacgg tttcttttgc
      721 aggaaaggaa tatgagttaa aggtcattga tgaaaaaacg cctattcttt ttcagtggtt
      781 tgaacctaat cctgaacgat ataagaaaga tgaggttcca atagttaata ctaagcagca
      841 tccctattta gataatgtca caaatgcggc aaggatagag agtgatcgta tgataggtat
      901 ttttgttgat ggcgattttt cagtcaacca aaagactgct ttttcaaaat tggaacgaga
      961 ttttgaaaat gtaatgataa tctatcggga agatgttgac ttcagtatgt atgacagaaa
     1021 actatcagat atttatcatg atattatatg tgaacaaagg ttacgaactg aagacaaaag
     1081 agatgaatac ttgttgaatc tgttagagaa agagctgagg gaaatttcaa aggcgcagga
     1141 ttctttgatt tctatgtatg caaagaaaag aaatcatgca tggtttgatt tcttcagaaa
     1201 tttagcctta ttaaaagcag gagagatatt caggtgcaca tataatacaa agaatcacgg
     1261 tatttcattc ggggaggggt gtatctatct tgatatggat atgatactta caggtaagct
     1321 tggtacaata tatgctcctg atggaatttc aatgcatgtg gatcgtcgta atgatagtgt
     1381 aaatattgaa aatagtgcaa taattgttaa ccgtagtaat catcctgctc tacttgaggg
     1441 actttctttt atgc
//
LOCUS       DAZFBR010000326         1449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFBR010000326 DAZFBR010000000
VERSION     DAZFBR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.329"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..803
                     /locus_tag="ACHJZ2_005257"
     CDS             <1..803
                     /locus_tag="ACHJZ2_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4571140.1"
                     /translation="RGIISDRLATAILRHAGRVALNAGASSTVSEVRPSSPSGAEATG
                     ITTLLSYLASESEGSLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEAGKSSWVLEYA
                     AGNGAALLQKGGQIRCRFKVSGALAANQYVMAFYWPVSSLPQGVALTGDGGNNLLAAF
                     YIQTDAKDLNVMYHNAKVATNNLKLGSFGAFDNEWHALAFRFAGNNSLQVTPVIDGQD
                     GTPFTLTQSPVSAFAADKLHVTDITRGATYPVLIDSIAVEVNSTDTAA"
     gene            941..1120
                     /locus_tag="ACHJZ2_005258"
     CDS             941..1120
                     /locus_tag="ACHJZ2_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4571141.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            1161..1406
                     /locus_tag="ACHJZ2_005259"
     CDS             1161..1406
                     /locus_tag="ACHJZ2_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4571142.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
BASE COUNT          368 a          342 c          438 g          301 t
ORIGIN      
        1 gccgtgggat tatttccgac cgtctggcaa cggcgatttt gcgccatgcg ggaagagtgg
       61 cgctaaacgc gggggcatca tcgacagtat cagaggtgcg cccgtcatcg ccttccggtg
      121 cagaagccac aggcatcaca acactgctct cttaccttgc cagcgagtca gagggaagcc
      181 tgaaagtaca gggatggtca gccagtggcg gcagggcaga agtggtcagc gatgcggagg
      241 gaaccggagg taaggcagtg aagctgacca aggaagccgg taaaagcagc tgggtgctgg
      301 agtacgccgc gggcaacggt gcggctctgt tacagaaagg ggggcagatt cgctgccgct
      361 ttaaggtttc gggagcgctg gctgcgaacc agtatgttat ggcgttttac tggccggtat
      421 cttcactgcc acagggcgtt gccctgaccg gagacggggg gaataacctg ctggcagcgt
      481 tctacatcca gacagatgca aaagacctga atgtgatgta ccacaatgcg aaagtggcga
      541 caaacaacct gaaactggga agctttggcg catttgataa cgaatggcat gcgctggctt
      601 tccgctttgc cgggaataac agccttcagg tgacgccggt tattgatggt caggatggta
      661 caccgttcac gctgacgcag tcaccggtca gtgcctttgc ggcggataaa ctgcatgtga
      721 cagacattac cagaggtgcg acttacccgg tactgataga cagcattgcg gtggaagtga
      781 acagcacaga cactgcggca tgataaaaaa aaccgccagc gacaggaatg gacgctggcg
      841 gtggtaatac ctatggagaa aaaataaagg aacgatactt tcgtgctctg gttttttaaa
      901 tgaaaacagt tcttattgtc aacaataacg gaaagaaatt atgacatttc tgaaccagtt
      961 aatgctgtac ttctgtacgg tggtctgtgt gctgtatctc ctttcgggtg ggtacagggc
     1021 catgcgtgac ttctggcgca gacagattga caaaagggcc gctgagaaaa tcagcgccag
     1081 tcagtcagcc ggaagcaaac ccgaagagcc gctcatttag cggcaacttt cttaatcaca
     1141 tctttcgacg agaaaatccc atgtcagaaa ttacatccct ggtcactgct gaagcagtga
     1201 aggaagtcct gcgctctgaa gaagtccgga gcgcactgaa acagaaactt cgccataacc
     1261 tggaagcgcg tcttgatgca gaagtggatg ccattctgga tgaactgctg ggcgcaccgg
     1321 cagctccgga gccggaaggc atcgcgggtg aggggagtgc ttcagatagc ggtgacccca
     1381 caccggacag cgacatgatg atgtaagcat gcgtcaggga ccatcggtgt gtgccggtgg
     1441 tctttttta
//
LOCUS       DAZFBR010000327         1437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFBR010000327 DAZFBR010000000
VERSION     DAZFBR010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.330"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..230)
                     /locus_tag="ACHJZ2_005260"
     CDS             complement(<1..230)
                     /locus_tag="ACHJZ2_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin subunit A"
                     /protein_id="HFO4571143.1"
                     /translation="MTSAKRNTLYMKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQS
                     YVSSLNSIRTEISTPLEHISQGTTSVSVINHTP"
     gene            complement(293..369)
                     /locus_tag="ACHJZ2_005261"
     tRNA            complement(293..369)
                     /locus_tag="ACHJZ2_005261"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(333..335),aa:Arg,seq:tct)
     gene            complement(383..459)
                     /locus_tag="ACHJZ2_005262"
     tRNA            complement(383..459)
                     /locus_tag="ACHJZ2_005262"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(423..425),aa:Arg,seq:tcg)
     gene            complement(461..536)
                     /locus_tag="ACHJZ2_005263"
     tRNA            complement(461..536)
                     /locus_tag="ACHJZ2_005263"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(501..503),aa:Ile,seq:cat)
     gene            complement(850..1338)
                     /locus_tag="ACHJZ2_005264"
     CDS             complement(850..1338)
                     /locus_tag="ACHJZ2_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4571144.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYH
                     SVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPILDSAFKNRKRVEKIKH
                     VA"
     gene            complement(1329..>1437)
                     /locus_tag="ACHJZ2_005265"
     CDS             complement(1329..>1437)
                     /locus_tag="ACHJZ2_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571145.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGAWA"
BASE COUNT          400 a          339 c          320 g          378 t
ORIGIN      
        1 ggggtgtggt taataacaga caccgatgtg gtcccctgag atatatgttc aagaggggtc
       61 gatatctctg tccgtatact atttaacgaa gatacataac tttgttgagt cgaaaagtct
      121 atcgtaaatt cccgggaata ggataccgaa gaaaagccca gtaacaggca cagtacccat
      181 ttaaataata tacacttcat atacaaggtg ttccttttgg ctgaagtaat cagcaccaga
      241 cccggcgcag atataaaaaa ggcccgcaaa agcgagccag ggaaaataag tgtggcgcgt
      301 tgtactggat tcgaaccagt gaccgattgc ttagaaggca attgctctgt cctgctgagc
      361 taacaacgca gaataccgat aatggaccac caccggggac tcgaacctcg cgcaaccagc
      421 ttcgaaggct gacgctctat cccgatgagc taatggcggt tggtggccct tgctggattt
      481 gaaccagcga cctggcgatt atgagtcgct cgctctcacc actgagctaa agggccgagc
      541 gtaggataat aacgttacga aatcaatgtt gcaagagttc acaaattcac tgattcaaaa
      601 ttattcttgt aaaaaatctt ttaacgtcag gcacaaagag cctgtacata gatttgtgta
      661 attgcctgat tttgatatgt tcaatccagc atcaaatgaa ggttaattta tggacgaaaa
      721 acagttacag gctctaatga cctttatacg attgacccaa ataaaaaaag ccaccgttgc
      781 aacttaagag tcactaacgg cagcttatgc gaatagtgtt gctcatttgc tcaacgatgt
      841 caacacgttc tatgctacat gcttaatttt ctctacacgt ttccggtttt taaacgcact
      901 atccagaatc gggtaaatca taaacaacga ggcattgagg atttcgtcaa cttcccgtcg
      961 gcaggttgct agcgatggtt tttgaatgcg cccgccgccc cggcataaca tcttgcgagg
     1021 tcttgcgacg ctatgatagt aagatgcaat ggcgtgcttg gaagagccgt gggcgtagta
     1081 gctgaggagg atgccaaagg ctttcttgtc aatgtacatg acggaatcga cgacctgaga
     1141 aatcaacatt ccatcatcat cattacacat tggccttgtc ataactcttc ccggctccac
     1201 gctctccatg aatttagcta ttacgctgct catgcgcttt tccagacgac ctgaataaac
     1261 ccatgcgccc cacagttcaa gccagccatt cagccactca tgctgttctt tggtgaggtt
     1321 tagttctctt atgcccacgc gccttctccc tgtacctgaa tcaatgtgag atttccgcag
     1381 aacactgcgc cggtatcgat atacatctgg ttggcaaatt tgagtggttt cactgct
//
LOCUS       DAZFBR010000328         1435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFBR010000328 DAZFBR010000000
VERSION     DAZFBR010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.331"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            173..>1435
                     /locus_tag="ACHJZ2_005266"
     CDS             173..>1435
                     /locus_tag="ACHJZ2_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO4571146.1"
                     /translation="MEYRFSWVIFNMINNISDQASSFPCTQLNQSDNFLDSLREFFAI
                     LNSSRKGELSTWDTIYLHLILVINADSDLIKNDVLLAENIPTADYQFNTFFSNAFETD
                     VKKYLGKSEDNEVEIKTGNERISIGIRNRSNGKLERQKFLFPLDYENKLQHQLDKHFT
                     IESHPLLYRHTMGSKIANVIFEKLYSRIDFNKEQYISFIKDAFLHFYDYSRRYAINEN
                     IAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEESFETALDVEHTIVLGFADDNFETK
                     PVHYQDLLTRFSAFQDTVFNLFPEMHSSHYNDICSVSVDMTKGTQCMIHLMVNEEVFM
                     SLPVPVATMVREDASNLINLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNES
                     ILKKCCFENGSLGDVKITNSNIVNNVFKN"
BASE COUNT          500 a          195 c          265 g          475 t
ORIGIN      
        1 ccagctttga cttttgataa tattatttat gctacagttg tgggtataaa atataatctt
       61 tttgaacaag tgaatttgag ctaagaataa atcaagttgt ttcagtgttt cgttaattaa
      121 tatgagtata aaaggtgttg tgcttttata ataataaagc gcgaggagtt cggtggagta
      181 tcgtttttct tgggtgattt ttaatatgat aaacaatata tctgatcaag cgtcatcatt
      241 tccatgcaca caattgaatc aaagtgataa tttcctcgat tcactgaggg aatttttcgc
      301 gatattaaat tccagtcgta agggtgagtt gtctacatgg gataccatct acttgcatct
      361 gatattagta ataaatgctg acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa
      421 tattccaaca gcggattatc aatttaatac attttttagt aatgctttcg agactgatgt
      481 taaaaaatat ctgggtaaat cagaagataa cgaagttgaa ataaaaacgg gtaatgaaag
      541 aatctctatt ggaatacgta acagatcgaa tggaaaactt gaaagacaaa agtttctttt
      601 cccactggac tatgagaata aattacaaca tcaattagac aagcatttta cgattgaatc
      661 gcacccatta ttatacaggc atactatggg tagtaagatt gcgaatgtga tttttgaaaa
      721 attatacagt cgtattgact ttaacaaaga acaatatata tcctttataa aggacgcgtt
      781 tcttcatttt tatgattata gcaggcgata tgcaattaat gaaaatattg caaaagacgc
      841 tgtaacaaac aatatcgcat tgatgagcac tttttatgac agtgataata cgtcaggtga
      901 ggttttaaat aatgatttta cagaagagtc atttgaaact gcgttagatg tagagcatac
      961 aatagtgctt ggatttgctg atgataattt tgaaacaaaa cctgttcatt atcaggattt
     1021 attaacacga tttagtgcct ttcaagatac cgtgtttaat ctgtttcctg aaatgcactc
     1081 ttcgcattac aacgatattt gtagcgtaag tgtggatatg acaaagggaa cgcaatgcat
     1141 gattcacttg atggtgaatg aagaagtttt catgtctctg cctgttccag ttgccacgat
     1201 ggtacgtgaa gatgcaagta atttaatcaa tcttaaaaca ctactcaatg atggttgctt
     1261 tattaaatat agtcatttta atgacgtggc attaataaag caaaacataa gtaatctcta
     1321 tttatcacac actgttgtga atgaaagcat attgaaaaaa tgttgttttg aaaacggcag
     1381 tttaggtgat gttaaaataa caaatagcaa tatagtaaat aatgtattca aaaat
//
LOCUS       DAZFBR010000329         1422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFBR010000329 DAZFBR010000000
VERSION     DAZFBR010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.332"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            79..570
                     /locus_tag="ACHJZ2_005267"
     CDS             79..570
                     /locus_tag="ACHJZ2_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571147.1"
                     /translation="MKPRNINNSLPLQPLVPEQENKNKKNEEKSVNPDKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYKFPITDDLMSMAVNELIKLT
                     KIENNVDLNKFTTICTDVLSPLVTKHNKQKSQNDILPFAKIPFLIFIEKMEIDNMNPG
                     HVH"
     gene            796..1287
                     /locus_tag="ACHJZ2_005268"
     CDS             796..1287
                     /locus_tag="ACHJZ2_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4571148.1"
                     /translation="MDTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNVTDAEIYYKLLCIYAFENHEYLKGFASVCQSKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDASVKSKAQAYIELLTDNSE
                     DNG"
BASE COUNT          568 a          215 c          228 g          411 t
ORIGIN      
        1 catgagaaaa ggtgatattt gacattatca gaagctgcga attcggattt tgctctaatc
       61 aaatgagaga aatgtaaaat gaagccacga aatattaata atagcctacc actgcaacca
      121 ttagttcctg aacaggagaa caaaaataag aaaaatgaag agaaatccgt taatccagat
      181 aaaattacaa tggggtctgg tttaaattat attgaacaag aatctcttgg agggaaatat
      241 ctaacacatg atttgtcaat aaagatagcg gatatttctg aagagataat tcagcaagca
      301 atattatctg ctatgagcat atataaattt ccgataacag atgatttaat gagtatggct
      361 gtaaatgaac tcataaaact gaccaaaata gagaataatg tagacctgaa taaattcact
      421 actatatgca cagacgttct atcccccctc gtcaccaaac ataataaaca aaaaagtcaa
      481 aacgacattc tacccttcgc aaaaatcccc tttttaattt tcattgaaaa aatggaaatt
      541 gataatatga atccaggtca tgtacattaa ggcattttga aatgtcataa ttaggataac
      601 taataaggtg aatattagta taacagctga tgatgttatt aatttgtagt ggaaagtatt
      661 ttaactaata atgaagaaat atactcataa gcaatgcaag cttaaaactc ataatttaaa
      721 aaagataaat ataaaaatat caatgagtca atcaataata atttagcatc acgagacaca
      781 tcacagagga atattatgga cacagaaaca attgaaatat tcaataatag tgatgaatgg
      841 gcaaatcaac taaaacacgc attatcgaaa ggagaaaatc tggcattact acatggtttg
      901 actcctgata tccttgatag aatatatgca tatgcattcg actaccatga aaagggtaat
      961 gtaacggacg cagaaattta ttataaatta ttgtgcattt atgcgttcga aaatcatgaa
     1021 tatctaaaag gttttgcatc agtatgtcag tcaaaaaaga aatatcaaca agcatatgac
     1081 ctttacaagc taagttacaa ttactccccg tatgatgact attcagttat ttatcgtatg
     1141 ggtcaatgtc aaattggggc taaaaatatc gataacgcaa tgcaatgttt ctatcatatt
     1201 attaacaatt gtgaggatgc tagtgttaag agtaaagcgc aggcatatat tgaactctta
     1261 accgataatt cagaagataa tggctaagca ttacaatcat ttggttaaaa atgctggtta
     1321 tagtgtagta accactatga ccagcacctc ttatgctttc ttaaatcaac atcttaaaac
     1381 agttaagaac acactaaaaa atattatttt tcaacgcgtt ag
//
LOCUS       DAZFBR010000330         1408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFBR010000330 DAZFBR010000000
VERSION     DAZFBR010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.333"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..291
                     /locus_tag="ACHJZ2_005269"
     CDS             <1..291
                     /locus_tag="ACHJZ2_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4571149.1"
                     /translation="NPEMPPPAVISHGIEYMEDGLPDPVKAMGQILVENLIIDPKLAL
                     DAAWRLAQFTHHKKGDAGKKSAKGDAAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            288..>1408
                     /locus_tag="ACHJZ2_005270"
     CDS             288..>1408
                     /locus_tag="ACHJZ2_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4571150.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILLRADVVTVGIDGGGLDDLLGMYVIGRDR"
BASE COUNT          315 a          355 c          442 g          296 t
ORIGIN      
        1 aatccggaga tgcctccgcc cgcggtgata tctcatggta ttgaatatat ggaggatggt
       61 cttcccgatc cggtgaaagc tatggggcag atcctggtgg aaaacctgat aattgacccg
      121 aaactagcac tggatgcggc ctggcgactg gcgcagttca cacaccataa aaaaggcgat
      181 gccggtaaaa aatcggcaaa aggtgatgcc gcgaaaaaag cggctaaccg ttttgcggtg
      241 ccaccgccac cccgactggt ggtgaataac gataatgagg gcaacggatg atacctgtat
      301 ggagcactgc ctgcccggac tgggcagagc gcctgaaaaa ggggctgtcg attattccgg
      361 ctccgattta tccggaccag gctgcacatg cactggcgat ttttaaacaa ctgcggattg
      421 tggatgcacc gggtagcccg acgttcgggg agtcctgtgc accgtgggtg tttgacctgg
      481 tggcggccct gtttggctcc tacgatgcgc agaccggtgt tcgccatatc aaggaagttt
      541 ttatccttat ccccaagaaa aactcgaagt ccacgctggc cgcagggatc atgatgactg
      601 cactgttact gaactggcgg caggcggcgg gctacacgat tctggccccg acagtggagg
      661 tggcagccaa cgccttcaac cctgccaggg atatggtacg acgggacgat gatctggatg
      721 acctctgtca ggtgcagaca catatccgga ccatcaccca ccgggtgaca gacaccaccc
      781 tgaaggtggt ggcagccgat ccgaatacgg tgtccggtat caagtccgtg ggtacgctga
      841 ttgatgagct gtggctgttt ggcaagcagt acaaggcgga ggacatgtta cgtgaagcca
      901 tcggtggcct tgcctcccgc ccggaagggt ttgtggtgta tacgaccacc cagtcgaatg
      961 aacctcccgc cggggtgttc agacagaaac tgcagtacgc ccgggatgtg cgcgacggca
     1021 aaattcatga tccgcacttt ctgccggtga tctttgaaca ccctcctgaa atggtggaaa
     1081 gcggggctca cctgctgatg gaaaacctcg ccatggtcaa tccgaatctc ggctattcag
     1141 tggatgaggc ctttctgtac cgggagtatc gaaaagcacg ggaagccggt gaagagacat
     1201 tccggggctt catgtcaaaa cacgccaatg tggaaattgg tcttgccctg cgctctgacc
     1261 gctgggcggg ggcggatttc tgggaagagc agggccgttg tatcagcctg gacgatatcc
     1321 tgctgcgtgc tgatgtggtg acagtgggga ttgacggcgg agggctggat gatctgctgg
     1381 ggatgtatgt gattgggcgt gaccggga
//
LOCUS       DAZFBR010000331         1399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFBR010000331 DAZFBR010000000
VERSION     DAZFBR010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.334"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(275..817)
                     /gene="ybfC"
                     /locus_tag="ACHJZ2_005271"
     CDS             complement(275..817)
                     /gene="ybfC"
                     /locus_tag="ACHJZ2_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO4571151.1"
                     /translation="MIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFPVLNNKAMWR
                     WYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGTLQELINTAE
                     KTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYARMTAHLPNK
                     DESYECLTKIQHELLRSEEK"
     gene            complement(842..>1399)
                     /locus_tag="ACHJZ2_005272"
     CDS             complement(842..>1399)
                     /locus_tag="ACHJZ2_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4571152.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGG
                     YGARPNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKW
                     VKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGP
                     ATAGIDDLGEILAKMKQRTSRGIRK"
BASE COUNT          380 a          292 c          244 g          483 t
ORIGIN      
        1 ccatttcacg actatttact taaatttcca ctggattttc ttttccaaat actgagtgtg
       61 atattgtaaa caatatcata ataagtaata ttagtgagtt tctcatgttc tacacctatc
      121 gaatctcaaa agttgtgggt aaaacattac attcagaata tatgatattc ttaccatcgg
      181 aatccatttt tccaaatcga ccttctggtg tacctggata tccattaaga acatgttttt
      241 ccgaatggtt aatttatttt ctcaggaaga tgttttattt ttcttcacta cgaagcagtt
      301 catgctgtat ttttgttaaa cattcgtaag actcatcttt gttcgggaga tgtgctgtca
      361 ttcgagcata agttggcttc tcatctttaa aatatttaac catgattgaa ttatcaagga
      421 ctgccaacaa ctgagctgga tcactgctct ttttttgata gatacccgct cttatataac
      481 ttctaaaccg atcgccgaga aacgccgttt tttctgctgt atttatcaac tcctgcaacg
      541 ttcctttgat ggcatgattt tcattaaggc gtagtgaccc gacacgaatt aagagtctcg
      601 ccccgcttgg tgtgaattta ttatttttac atgtacccag ttctgtttgc caataatact
      661 caccgatgtc ctcattctga taccatctcc acatggcttt attatttaag acaggaaacg
      721 cggaaaaaat agtaccctta gcgaaaatag taaaacaatc atgatcttta gcttgtattt
      781 cgcaatcagc tataacacca aaactaacaa atatcatcag caaaaaaaaa caaaactctt
      841 ttcattttct tattcctctc gatgttctct gtttcatttt agctaaaatt tcacccaaat
      901 catctatccc tgctgttgct ggcccatcgg gtttattaat gtcagggaat aaatagggtt
      961 ctgcacaagt atatcctggt ggtaattgag ttgttggcca atttgtctgt ggcaggtaat
     1021 cacttgctgt ctttgtatcc atccctggat actggggatc atcctgtggc gtacaagtca
     1081 gatttggttt tacccacttc ctgcaataca gtgtgtagtc gtaagggctt aatgctctga
     1141 agaatgtttt gaaaacatta ggcgcactac tggcctggtc aataggatat ggcagtctta
     1201 attgtttcgc tatgtctgga aatttacttg gatcgggcgt aggcggtttg ttgggtctcg
     1261 ctccatatcc gccattattt aagtgtccat attttgatgc cagtcccatt gaatctatat
     1321 actgaaccgg attcaacgga tactgataaa aattccatcc ccccttcagt ccaatcggat
     1381 cctgagtgat atacctccc
//
LOCUS       DAZFBR010000332         1394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFBR010000332 DAZFBR010000000
VERSION     DAZFBR010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.335"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            162..>1394
                     /gene="pitB"
                     /locus_tag="ACHJZ2_005273"
     CDS             162..>1394
                     /gene="pitB"
                     /locus_tag="ACHJZ2_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO4571153.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPGNTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGE"
BASE COUNT          319 a          331 c          360 g          384 t
ORIGIN      
        1 tgttctaatt atttctaaag atttttaatg aatgcttatt gtctgataca caagaaataa
       61 cactcttttt atcgttaaaa aatgatattt cactttgccc acaccgttaa agtcactgat
      121 aatgcgtccg ttcgtaaatt caaaatggcg taatctaata tatgctaaat ttatttgttg
      181 gccttgatat atacacaggg cttttgttat tacttgctct ggcatttgtg ttgttctacg
      241 aagcaatcaa tggttttcat gacacggcga atgcggtggc aaccgttatt tatactcgtg
      301 ccatgcagcc acagcttgcc gtggtgatgg cggcattttt taactttttt ggcgtgttat
      361 tgggcggact tagcgttgcc tatgccattg tccatatgtt gccaaccgat ttgttgctga
      421 atatggggtc aacccacggc ctggcgatgg tcttttccat gctgctggcg gcgattatct
      481 ggaacctggg aacgtggttc tttggtttac cggcctccag ttcgcatacc ttgattggcg
      541 cgattatcgg catcggttta accaacgcgc tgttaaccgg ctcatcggtg atggatgcgt
      601 taaacctgcg tgaagtgacc aaaattttct cctcgctgat tgtttcccct atcgtcggcc
      661 tggtcattgc gggaggcctg atattcctgc tgcgacgcta ctggagcgga acgaaaaagc
      721 gtgaccgtat tcaccgcatt ccggaagatc gcaaaaagaa aaaaggcaaa cgtaagccgc
      781 cattctggac gcgtattgcg ctgattgttt ccgctgcggg cgtggcgttt tcgcacggcg
      841 cgaacgacgg acaaaaaggg atcggcctgg tgatgctggt actggtgggg attgcccctg
      901 ctggcttcgt cgtcaatatg aacgcgtccg gctatgaaat tacccgtacc cgcgatgccg
      961 ttaccaactt cgaacactac ctgcaacagc atcctgaact gccgcagaag ttgattgcga
     1021 tggaacctcc attgcctgcg gcatctactg atggcgcgca agtgacggag tttcactgtc
     1081 atccgggaaa tacctttgat gcgattgcgc gcgttaaaac gatgctgcca ggcaatatgg
     1141 aaagttacga gccgttaagc gtgagtcagc gcagccagct gcgccgcatt atgctgtgca
     1201 tctctgatac ttccgcgaag ctggcgaaac tgccaggcgt cagtaaagaa gaccagaacc
     1261 tgctgaaaaa actgcgcagc gatatgttaa gcaccattga gtacgctccg gtgtggatca
     1321 tcatggcagt agcactggcg ctcggcattg gcaccatgat tggctggcgt cgtgttgcga
     1381 tgaccatcgg tgag
//
LOCUS       DAZFBR010000333         1386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFBR010000333 DAZFBR010000000
VERSION     DAZFBR010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.336"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            90..1318
                     /locus_tag="ACHJZ2_005274"
                     /pseudo
     CDS             90..1318
                     /locus_tag="ACHJZ2_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          368 a          322 c          396 g          300 t
ORIGIN      
        1 gactggcccc ctgaatctcc agacaaccag tatcacttaa ataagtgata gtcttaatac
       61 tagtttttag actagtcatt ggagaacaga tgattgatgt cttagggccg gagaaacgca
      121 gacggcgtac cacacaggaa aagatcgcaa ttgttcagca gagctttgaa ccggggatga
      181 cggtctccct cgttgcccgg caacatggtg tagcagccag ccagttattt ctctggcgta
      241 agcaatacta ggaaggaagt cttactgctg tcgccgccgg agaacaggtt gttcctgcct
      301 ctgaacttgc tgccgccatg aagcagatta aagaactcca gcgcctgctc ggcaagaaaa
      361 cgatggaaaa tgaactcctc aaagaagccg ttgaatatgg acgggcaaaa aagtggatag
      421 cgcacgcgcc cttattgccc ggggatgggg agtaagctta gtcagccgtt gtctccgggt
      481 gtcgcgtgcg cagttgtacg tcattctcag acgaaccgat gactggatgg atggccgccg
      541 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
      601 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
      661 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
      721 tgagcgaaaa cctactgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa
      781 agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag
      841 actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact gggcggtgac
      901 taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
      961 cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta
     1021 ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc
     1081 ggtgcggagt ccggagagta acggaatagc agagaacttc gtgaaaacga taaagcgtga
     1141 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
     1201 gttcgagcat tataacgaat ggcatccgca tagtgcgctg ggttatcgct cgccacggga
     1261 atatctgcgg cagcgggctt gtaatgggtt aagtgataac agatgtctgg aaatataggg
     1321 gcaaatccag atggatttat cgtaataaag acaacagcca aataatgaaa aacataaaat
     1381 tttatc
//
LOCUS       DAZFBR010000334         1378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFBR010000334 DAZFBR010000000
VERSION     DAZFBR010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.337"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..403)
                     /locus_tag="ACHJZ2_005275"
     CDS             complement(<1..403)
                     /locus_tag="ACHJZ2_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571154.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAI"
     gene            complement(656..1279)
                     /locus_tag="ACHJZ2_005276"
     CDS             complement(656..1279)
                     /locus_tag="ACHJZ2_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4571155.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            complement(1276..>1378)
                     /locus_tag="ACHJZ2_005277"
     CDS             complement(1276..>1378)
                     /locus_tag="ACHJZ2_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571156.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA"
BASE COUNT          401 a          351 c          272 g          354 t
ORIGIN      
        1 caatcgctgc tgacagtagc aaaagaggta cctgaatctt ctgtctcaat atagaaaact
       61 caataattgc cggcacaaac aataattcca cagcaaaata aaggcgaaat acatttagct
      121 cttgcataga atgttttctt ttcactgcga aaaagaatac aacaccaata ccccaaccga
      181 taagaaatat agcaatgacg ataactgcaa aaaataaact tctggcaaca tcatcaacac
      241 ctgcacctac aatccaccat gggaagccgt agtaaaaaga agtaccccat ccatagaaat
      301 aagcactccc ccatccaagg catcccatgt aggcaataaa aagtgaagaa ctcctgagca
      361 gcgcaccatc cttcataacc accccaatac aagatgataa cattggctta caactcataa
      421 caaaagcaat tcaatgccgt caagaggtta caggctaaaa aaactctatt acattgcagt
      481 cagcatgttt actacacaaa tacaattcag agcataaaaa ctactcggcg gcaggttatt
      541 gagactcatc aatgacatgt aaaaaacgcc cattattggt gtcaagtttc cccaaagtta
      601 ttcaaaaagt caatattatg ccgttaatat gttgccatcc gtggcaatca tggcgctaac
      661 gtgtgatcgc attcaaaatg ttgtctgcga ttgactcttc cttgtggcat tgcacaacca
      721 gagcgtcata cagcggctta acagtgcgtg accaggtggg ttgggtaagg tttgggatta
      781 gcatcgtcac agcgcgatat gcggcgcttg ctggcattct tgaataaccg acgcctttac
      841 atcttccgca ctctttctca gcaactatcc cccactgctc tgttttggct atatcaaccg
      901 cacggcctgt accgtggcaa tctctgcatc ttgcgcccgg cgtagcggca ctacggcaat
      961 aatccgcata agcgaatgtt gcgagcactt gcagtacctt tgccttagta tttccttcaa
     1021 gctttgccac gccacggtat ttccccgata ccttgtgtgc aaattgcatc agatagttga
     1081 tagccttttg tttgtcgttc tggctgagtt cgtgcttacc acagaataca gccattccga
     1141 atccggcttg tgattgcgcc atccccatag cagccatcac atcagtaccg gaaagagagt
     1201 cagaagccgt ggcccgtggt gagtcgctca tcatcgggct ttttggcgaa tgaaatttag
     1261 ctacgctttc gagtctcatg cgccttctcc ctgtacctga atcaatgtga ggtttccgca
     1321 gaacactgcg ccagtatcga tatacatctg gttggcaaat ttgagtggtt tcactgct
//
LOCUS       DAZFBR010000335         1365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFBR010000335 DAZFBR010000000
VERSION     DAZFBR010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.338"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..359)
                     /locus_tag="ACHJZ2_005278"
     CDS             complement(<1..359)
                     /locus_tag="ACHJZ2_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleG8"
                     /protein_id="HFO4571157.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMDRRAVALERQLNGGVDFLSSVNNYFQSVMA
                     EHRENKTGNKILMEKINS"
     gene            complement(432..1004)
                     /locus_tag="ACHJZ2_005279"
     CDS             complement(432..1004)
                     /locus_tag="ACHJZ2_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4571158.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGQGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPEKGVFVKNSDGSDVCTLFDAAAFSRLTGEDLPHPL
                     TREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            complement(1037..1114)
                     /locus_tag="ACHJZ2_005280"
                     /pseudo
     CDS             complement(1037..1114)
                     /locus_tag="ACHJZ2_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(1116..1364)
                     /locus_tag="ACHJZ2_005281"
     CDS             complement(1116..1364)
                     /locus_tag="ACHJZ2_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4571159.1"
                     /translation="MQRLCGCGKHDDCEHGQSLTVQLRLGPADILESDENGIIPEQVR
                     VITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          379 a          364 c          226 g          396 t
ORIGIN      
        1 gaatttattt tttccattaa tattttatta cctgttttat tttctctgtg ttctgccatg
       61 acactctgaa aatagttatt aacactactt aaaaaatcga cacctccatt taattgcctt
      121 tccagagcca cagcccttct atccattcga tgctcacgcc ccaatagcct atccagtaag
      181 cctcctctca ccggttcaac tgaaaatcca tccagtagtt ggacatagtg aacagaatat
      241 tggccactac caatggatat tcttattcca ctttcaagtc tggcagcatc ctgcaaagac
      301 tgtactcctg caggagataa ttgagatata cttgggatac ttaaggtggt aacaggcata
      361 aaaacctcat tcagtaattc atatcagttt gcaggatgtt tcattaaata aaataaaggg
      421 taatgttcat ttcaattacc ctttataacg aagtttcctc tggtatcgtc ataaatgcat
      481 tcttcatgtt ttacaattat tgatgccgtt attggttccc gggtcagtgg gtggggtaag
      541 tcttcaccag tcaaacgaga aaatgcagcg gcatcaaata aagtacatac atctgaacca
      601 tctgaattct tcacaaaaac acctttttca ggctgctcca gtgtaattgg acattgaagc
      661 gcatcaccag gacattgaag tttttcaata tcgacagtaa aggcacactc cgatattttt
      721 tcctgcaaag agtttcgccc tcccattcca gggagcgcac ttaatatcct gtttctgagc
      781 acattaactc catcattaaa agcaatattc aataattctg cataaacccg tccctgccct
      841 tcactttctg ctaaaaaacg gcctgtatct ggcatataag aaactctaac cctctcccct
      901 cccaggagaa atccaccaat ctgcccctcc attacctgct gacgcagctc catcatctca
      961 ttcacgaatg aagccgatga cacatatgat attaatccag gcattaccat gctccttcat
     1021 acggataaat attccctcaa gtcttcatta acaaacatcc accggacatg acaacaaaaa
     1081 ccggagccgg actccggttt tgtgaagctg tcgggttact tcatcccgcc aatattttcc
     1141 cacgtcccgt cagcacgcag gatttgcagc ggtcttacca cacactgtat ctgcttttta
     1201 tccgcatcca gtatcaccac ctgcgtgatt accctgacct gctccgggat aatgccattc
     1261 tcatctgact ccaggatgtc tgccggcccc agtcgcagtt gtactgtaag cgactgcccg
     1321 tgttcacagt catcatgctt tccgcaaccg cacagacgct gcata
//
LOCUS       DAZFBR010000336         1351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFBR010000336 DAZFBR010000000
VERSION     DAZFBR010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.339"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..268
                     /locus_tag="ACHJZ2_005282"
     CDS             <1..268
                     /locus_tag="ACHJZ2_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO4571160.1"
                     /translation="TGFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPE
                     QLSKRERYSEKWQRRTHSAWRHRHWHVSAFTGIYNDLAAASACM"
     gene            265..846
                     /locus_tag="ACHJZ2_005283"
     CDS             265..846
                     /locus_tag="ACHJZ2_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4571161.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQR"
     gene            1095..>1351
                     /locus_tag="ACHJZ2_005284"
     CDS             1095..>1351
                     /locus_tag="ACHJZ2_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571162.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGA"
BASE COUNT          315 a          333 c          436 g          267 t
ORIGIN      
        1 caccggcttt taccgggtgc ccctgtcctc tgcacaggcg ggcgatatcc tgctgtgctg
       61 ctttggcgca tcggtggcca atcatgccgc catttactgc ggcaacggtg aactgcttca
      121 ccatctgcct gaacaactga gtaaacggga gaggtattcc gaaaaatggc aacgacgaac
      181 gcattcagcc tggcgtcacc gccactggca cgtatctgcc ttcacgggga tttacaacga
      241 tttagccgcc gcctcagcct gtatgtgaac acggcagcgg aagccatccg ggcgctgtcg
      301 ttacaggtgc cgggattccg ccgtcagatg aacgaaggct ggtaccagat acgtattcgc
      361 ggtgaggaca cggcaccgga ggcggtgtac gcccgtcttc acgaacctct gggtgagggg
      421 gcggtcatcc atattgtgcc gcgactggcc ggagccggaa agggcggact gcagattgtg
      481 ctgggggcgg cggccatcgt ggggtcgttc ttcactgccg gggcatcaat ggcgttatgg
      541 ggttcagccc tggcagccgg tggtttttct gccaccacga tgctgttttc actgggggcc
      601 agcatgatac tgggtggtgt ggctcagatg ctggccccga aggctaaagt accggagtac
      661 aaaagcacgg ataacggcag acagaacacg tacttttcct cgctggataa catgattgca
      721 caggggaacc cgatgccggt gccttacggt gaaatgctgg ttggctcacg gcgaatctcc
      781 caggacatca gtacccgtga tgaaggcggt gacgggaagg tggtggttat cgggcggcag
      841 aggtaaaaag aataaaaaaa tcccgcagtg accgaaggct gcgggaacag aaaatgaaga
      901 ttaaccacag ggagttttgt ttttattggc ccgaaaaaac tgtaacgccc gggaatgata
      961 tctgccacgg gggcgtacag aaaatgtgaa gaaattcaga aattttattc cgtcatgaca
     1021 caggcaccct ccggggtgcc tgtcgttttt ggggcataaa cagattcaga catcagacag
     1081 gagaggggga cagagtgggt aaaggtggcg gcaaggcgca cacgccggta gaggcaaagg
     1141 acaatcttaa gtccacgcag atgatgagcg tgattgatgc gattggtgaa gggccgattg
     1201 aaggtccggt gaaggggctg cagagtattc tggtgaacaa aaccccgctg acggacacgg
     1261 acggtaatcc cgtgatacac ggtgtgactg cggtctggcg tgccggggag caggagcaga
     1321 caccaccgga aggctttgag tcctccggag c
//
LOCUS       DAZFBR010000337         1336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFBR010000337 DAZFBR010000000
VERSION     DAZFBR010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.340"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            50..430
                     /locus_tag="ACHJZ2_005285"
     CDS             50..430
                     /locus_tag="ACHJZ2_005285"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4571163.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            427..774
                     /gene="tnpB"
                     /locus_tag="ACHJZ2_005286"
     CDS             427..774
                     /gene="tnpB"
                     /locus_tag="ACHJZ2_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4571164.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            824..>1336
                     /locus_tag="ACHJZ2_005287"
     CDS             824..>1336
                     /locus_tag="ACHJZ2_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO4571165.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSR"
BASE COUNT          321 a          379 c          365 g          271 t
ORIGIN      
        1 agacaaagtg gtgtccacca aataagtagt gggaaccaaa gtatcagata tgcagaaaaa
       61 tgtgactccc ggcaggcgaa aaggctgccc taattatcct cccgaattta aacagcagct
      121 cgttgctgcc tcctgtgaac ccgggatatc catctcaaaa cttgctcttg aaaatggcat
      181 taacgccaat ctgttgttca aatggcgaca acaatggcgc gagggaaagc tgctattacc
      241 ttcttcagag agcccccagc tacttcctgt gactctcgat gcagctgccg aacagccaga
      301 atcgctcgca gaggacccgg aaaccctcag tatcagctgt gaggtaacgt tccggcacgg
      361 gacgctccgc ttcaatggca atgtcagcga aaagctcctg actctgctga tacaggaact
      421 gaagcgatga tcccgttacc ttccgggacc aaaatttggc tggttgccgg tatcaccgat
      481 atgagaaatg gcttcaacgg cctggctgcg aaagtacaaa cggcgctgaa agacgatccc
      541 atgtccggcc atgttttcat tttccggggc cgcagcggca gtcaggttaa actgctgtgg
      601 tccaccggtg acggactgtg cctcctgacc aaacggctgg agcgtgggcg cttcgcctgg
      661 ccgtcagccc gtgatggcaa agtgttcctt acgcaggcgc agctggcgat gctgctggaa
      721 ggtatcgact ggcgacagcc taagcggctg ctgacctccc tgaccatgct gtaaatctct
      781 ttatcctggt tgtcacagaa taagcccggt aaaatacggg cttatgaacg acatctcttc
      841 tgacgacatc ttcctgctga aacagcgcct ggccgaacag gaagcgctga tccacgccct
      901 gcaggaaaag ctgagcaacc gggagcgcga aatagaccat ctgcaggcgc agctggataa
      961 actccgccgg atgaacttcg gcagtcgttc cgaaaaagtc tcccgccgta tcgcacaaat
     1021 ggaagccgat ctgaaccggc ttcagaaaga gagcgatacg ctgactggta gggtgtatga
     1081 cccggcagta cagcgtccgt tgcgtcagac ccgcacccgt aagccgttcc ctgaatcact
     1141 accccgtgac gaaaagcgac tgttgcctgc ggcgccgtgc tgcccgaact gcggcggttc
     1201 actgagctat ctgggcgagg ataccgccga acagctggag ttgatgcgta gcgccttccg
     1261 ggttatccgg acggtacggg aaaaacatgc ctgtactcag tgcgatgcca tcgtgcaggc
     1321 acctgcacct tcgcgg
//
LOCUS       DAZFBR010000338         1312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFBR010000338 DAZFBR010000000
VERSION     DAZFBR010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.341"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(39..227)
                     /locus_tag="ACHJZ2_005288"
                     /pseudo
     CDS             complement(39..227)
                     /locus_tag="ACHJZ2_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          385 a          290 c          237 g          400 t
ORIGIN      
        1 aaccagcaaa cgggcattgt gttctgaaat ccaggctatt gattcaacgt cagcgacaga
       61 tctgcgctgt aaaagaactt gtaaaacaac gtaatagaat tatccggaat ggtggcgact
      121 atgcactaga acaccacaat ttcgctctct cggtaaggca ctgtcacagc attcaacaga
      181 atgtgacttg ccagatttat tagcaccacc agatgtttaa ccgggtaacc acgacccagt
      241 cggggatata ttccatcttc tcgctgacat catcaagaat aaaaaggttt attacactca
      301 gagcaacgtt cttcagcagg cgggataatt cttcaacatt tagaagatgt gttattaaag
      361 gcatacaaac tggacgtatt gtattttctt ttggtggttg acctaaggta gcagtttatc
      421 ctgatgcgct gagatttctg taatatctac gtcaacattc tcctcgaata gaaaatgcag
      481 ccccggcaag taattcacat tttttaacaa aacgttgtta gcgtgccatt ttcagaatct
      541 ctccaagaaa gcgaattctt ttctcaaatt ctgcattctc agacacagct ttctactcca
      601 tggtaatggc ccaaatacga agttgctcag gatcattagt ggtgcttaga agttaaataa
      661 ccatgcggag taccactcgt catcacgtat ttatatatca tgaagctatt gttgtgtttt
      721 gtacatcagt agatatattc tgatatactc cttttgctag acataacctt tcacctgctt
      781 gcaaagcttc tgtgttctga cattgccaaa ttgttgcaat tctgtatcca gccttctttc
      841 agtcatagct tcgggccgcg ataagactca ctgatctgac cctgattcct cttgcagact
      901 ttatagacca attaaaatgc agtttctgca ggtcaacgtc tgaccatcat tgtcatcact
      961 ctggccatta gagtaacctt ctgcattcat ccttttgtaa aaagtttata ttagtatcag
     1021 caattaaccg gacctgatac tgatatgagt cttaccgcat atacggtcaa tttcagcaat
     1081 taattacatt atccacgcca aagtatttgt catcacaatg atggtacctt ctttcagaca
     1141 ccattttttc aactccgttt tccacggacc gcactcttat gtcaagagtg cggtccgtgg
     1201 atacaaccag agaccgactg acacgagtca gaggaaacga cggatatgtt cagtcgtaaa
     1261 atatctatca aaaaacatga ttaaggtcaa aaatgtttga tatttacaat tt
//
LOCUS       DAZFBR010000339         1311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFBR010000339 DAZFBR010000000
VERSION     DAZFBR010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.342"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            1..1300
                     /gene="ygeH"
                     /locus_tag="ACHJZ2_005289"
                     /pseudo
     CDS             1..1300
                     /gene="ygeH"
                     /locus_tag="ACHJZ2_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
BASE COUNT          476 a          226 c          225 g          384 t
ORIGIN      
        1 gaaatgagca agtttatatt ccaccgaaag agttaggtgt attaatagta ttacttgaat
       61 ccgctggtca tgtcgtactg aaagatatga tcatcgaatc agtatggaaa aatattattg
      121 ttagtgatga gtccctgaca agatgtatct attctttgcg ctgcattttt gaaaaaattg
      181 gctatgatcg ttgcatagaa acaatctacc ggaaaggtta tcgtttcagt gggcaggttt
      241 tcaaaactaa aataaatgaa gataacactt cagactattc tatcgctata ttccctttca
      301 ctacttcatt gaaaacactg gatccattaa tacttaatca ggaattagta caaatcattt
      361 caaataaaaa aatcgatggc ctctatacct atccgatggc tgcgacaaat ttttgtaatg
      421 atcacatatc gcaaaattca tttttgagaa gattcaatcc agattatttc gttacaggaa
      481 gaataaacca gaataatgca gtgaacactt tatacattga gttgaccgac gctaaaaacc
      541 ttttcctcat cgccagtaat catctacctg ttgatgaact acataataca tcacaattta
      601 ttatagaaaa tatccttcaa acagtacata atccacaacg atctgtaaga ttaactaagc
      661 aggaccaagg atataagaat cattatttat cagatgaaat gttagccgga aagaaagaac
      721 tttacgagtt cacccctgaa agcatttaca gggccatgac tatatttgat ggattacaaa
      781 ataaaagtga tatacagacg ctaaaaacag aatgttattg ccttctagcg gaatgccata
      841 tgtctttggc acttcatgga aaaagtgaac ttgaacttgc tgctcaaaaa gcattagagc
      901 ttttagatta tgtatcagac ataaccactg tcgatggaaa aattttagct attatgggac
      961 tgataactgg tctgtctgga caagcaaaag tatctcatat cttatttgaa caggctaaga
     1021 tacactcaac tgatatagcc tctctctact actatagggc acttgtcaac tttcataatg
     1081 aaaaaattga agaggcaagg atttgtatag acaaatcact acaactcgaa cccagaagac
     1141 gaaaagcagt tgtgataaaa gaatgtgtag atatgtatgt gcctaacccg ctcaaaaaaa
     1201 catgaaactc tactataaag aaactgagag tggaagccat cgagttataa ttgacaacat
     1261 tttgaaatta aagcagctga cgagaatttg tatgcgataa atttttattg a
//
LOCUS       DAZFBR010000340         1301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFBR010000340 DAZFBR010000000
VERSION     DAZFBR010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.343"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(274..969)
                     /locus_tag="ACHJZ2_005290"
                     /pseudo
     CDS             complement(274..969)
                     /locus_tag="ACHJZ2_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1092..>1301)
                     /locus_tag="ACHJZ2_005291"
     CDS             complement(1092..>1301)
                     /locus_tag="ACHJZ2_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4571166.1"
                     /translation="DFQQQGARGLTNAPDEDPDDLSTGYYGSAYRSPENWTTALRSSH
                     FSSAARRGIISDRFVEAILQFWRER"
BASE COUNT          318 a          316 c          341 g          326 t
ORIGIN      
        1 aatagtatcg tcaggacgat aaaaagataa tcatgacaaa ggcaattgaa ctaaaatgat
       61 tgacatttta caggtggttt gtcagtgatt tcttgttatt cataatttat tttccgtaaa
      121 aaatagtaag gtgatcacct gaataaaggt aatctcatat tttgatgagt atagtgattt
      181 tttatttctc tggtgaggaa tgaaggaaat atcaatggta aggaacgtca gagaatcagg
      241 gaaaattaag gtccctcaga aacacaatgc cgttcactta agcggggcgg cactgtgttt
      301 cacaggataa cgggtttttg atatcttaac cgccctcgcc cttgatggtc gggggcgttt
      361 tgttatgaac accacttcca gagcccccgc agatgctcca gtcgtttcgg gatagtcccc
      421 ggtgacgccg tgtttcccag cactatcatt tctgtcacga taaactggaa cgcgaactta
      481 aagctgatct ctgacgccgc ttttgttgcc tcacgccgta tcgctccgcc ggtaggcgcg
      541 gtcatggcat tcagtagaat gtgattccca gattcattaa cgccaccaga cgcatgacgg
      601 ggtaggcgct ctgccgctca gtggatgtgt tggcagagcc ataatattca cgcagctcgg
      661 gttcatcagg tgtcctgaac tgtgcgccat caatggcaaa aagttgcagg ccgtgccagt
      721 catccttcag gtaacgttcc gcgccccatg tctgtgtggt ctggcggaag agccattcca
      781 ccggggcggc ccccacgcgc tgacgcgcct gggtgacagc gctgcgggcg ccagcaggtt
      841 catccccgct tcgccatcca cgctcaggtt aagacgacga acggtcgcat gagcagaaag
      901 cgtcaggaag tgctgtatcc actcggtggg aaggtgctct gcaaacagtt gcgtagaggg
      961 tggtggcata agcggatggt cactgaaagt ggtataagaa aatggctcct tgttgtggaa
     1021 gtcgttatag tgccgcacgc cggaggagtt aaaagcgctg atggatgcga ataaatcaaa
     1081 ttaatgaacg ctcacctttc gcgccaaaac tgcaaaattg cttctacaaa cctgtcagaa
     1141 ataatccccc gacgggctgc tgagctgaaa tgactgcttc gcagtgccgt cgtccagttc
     1201 tccggtgacc ggtacgctga accgtaatat cccgtgctta aatcgtccgg atcttcatca
     1261 ggcgcgttcg tcagccctct tgcaccttgt tgctggaagt c
//
LOCUS       DAZFBR010000341         1297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFBR010000341 DAZFBR010000000
VERSION     DAZFBR010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.344"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1182
                     /locus_tag="ACHJZ2_005292"
     CDS             <1..1182
                     /locus_tag="ACHJZ2_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4571167.1"
                     /translation="RRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALI
                     SEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGR
                     YITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSG
                     DLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSG
                     VSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWV
                     N"
     gene            1194..>1297
                     /locus_tag="ACHJZ2_005293"
     CDS             1194..>1297
                     /locus_tag="ACHJZ2_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HFO4571168.1"
                     /translation="MKKYIFLVLFLLYGCSPVDIEPARQEVNIFRGLY"
BASE COUNT          333 a          259 c          404 g          301 t
ORIGIN      
        1 cggcgaatgg caaaacgggt ctggcggcgg gagcgtgacc tgacggggtg gatgtcgctg
       61 tcgcgtaaac cggaggtgac gtggtatggc tgggacggag acaggctgac gacggtgcag
      121 actgacacca cacgtatcca gacggtatac gagccgggaa gcttcacgcc gctcatccgg
      181 gtcgagacag agaacggcga gcgggaaaaa gcgcagcggc gcagcctggc agagacgctc
      241 cagcaggaag ggagtgagaa cggccacggc gtggtgttcc cggctgaact ggtgcggctg
      301 ctggacaggc tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
      361 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
      421 ccggcgcgaa aagttcattt ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
      481 agcgaagacg gcaatacggc gtggcgcggg gagtatgatg aatggggcaa ccagcttaat
      541 gaagagaacc cgtattacct gcaccagcca taccgtctgc cggggcagca gcatgatgag
      601 gaatcagggc tgtactataa ccggaaccgg tactatgacc cgctacaggg gaggtatatt
      661 acacaagacc ccattgggct ggcgggggga tggaatctgt ataattaccc actgaatccg
      721 ataataagga tggatccttt gggtttgtat aatttatatc aattattata tgatgtttgg
      781 catgatgatt catatggaac atcatcaatt gatattactg gcagtggaga tctaatatca
      841 ttaggtggtc atgcaggact tggcgttgcg tttgctaaaa agaaaggtga aatgttatct
      901 gatatttgta tttatgctac agcatgcgga catgcaggaa ttggtggtgg gataaatgcg
      961 gctatcacat attctgagac caagtcttta cctacatcgg gagtcagcaa ttcagtaggt
     1021 gtaacggttg gcggcggagt tggggggcat tttgcgtata cttatgtagt ggatgttgat
     1081 aatccagaat catcgacaga atctgttggt atcggtgcag gtgttgacgc ttcagttatg
     1141 actctggctt gtagaacgtg gcaagaatgc tgggtcaatt aacaggagga aatatgaaaa
     1201 aatatatatt tttagtttta tttttattgt atggttgttc tccagtggac atagagccag
     1261 ctagacaaga agttaatatc tttcgtggtt tatatca
//
LOCUS       DAZFBR010000342         1285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFBR010000342 DAZFBR010000000
VERSION     DAZFBR010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.345"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>1285)
                     /locus_tag="ACHJZ2_005294"
     CDS             complement(<1..>1285)
                     /locus_tag="ACHJZ2_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4571169.1"
                     /translation="QSFQSEIQRASRMGSEYYRTLQNGGRQAAAAAREQRRALAELNS
                     QLTEIRASAVGMAGAFAGAFATGHLISLADEWSSVNARLKQASQSSDEFSSSQKVLMD
                     ISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKISGASTAEAGSVI
                     TQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLKAMADDGQLTADKVVP
                     ALISQLEVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASGVTKTLSGVLNGVAGQ
                     IDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAEAQFRGTQIATAR
                     ARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARSAAQNALNSTTAVGSR
                     LMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARES"
BASE COUNT          255 a          447 c          319 g          264 t
ORIGIN      
        1 cagactccct ggcctgctcc tgattctggt acagcgtgta ccatgctgca gcccccagca
       61 tcaccagtcc gggtacgcca ccaaccagcc ccagcgcacc gctcatcaga cgtgagccca
      121 ccgccgttgt actgttcagc gcattctggg cggcgcttct ggcagcaata tttctgttca
      181 ggcgttcctg tgtggccgcc agacgggctt ctgcagcaat ctgcatctcc gtcccgcggg
      241 ctgccgccac ggcctgctga gcacggtaca cggctgctct tgcccgcgcc gtggcaatct
      301 gcgttcccct gaactgtgct tccgccagtg caacttcatt acgtgcagcc gtcacaagtc
      361 ctgccgtggc agacatcgct ccggaggcca tattgccaaa gtaccgggca accccgacgg
      421 caaccagtgc ccccacggct gttgccacat tatcaatctg tccggcaaca ccgttcagca
      481 cgccggagag cgttttcgtc accccgctgg cctcattcgc accacccacc caggccataa
      541 aggcgttttc cacctttgtg atcccgtcag agaccgtttc cggcatggcc gcgtattcat
      601 cacgcaatac ctccagctgg ctgattaacg caggaacgac tttatccgcc gtcagttgac
      661 catcgtccgc catcgccttc agatctttac gggccacgcc catacccgca gccagtgcac
      721 gtacgatccg gtcaccactt tcattgaccg aattaaattc ctcaccgcgc aacacaccct
      781 gtgccagcgc ctggctgaac tgggtgatca ccgaacccgc ctcagccgta ctggcaccgg
      841 agattttcag ccccgtggaa atggcctccg tcaccttcag cacatcatca gcactgtaac
      901 catattcacg cattgaggct gccgagcggg caaacagggc cgcattatcc gaaaaagcgg
      961 tacctgtccg ctgactgata tccatcagca ctttctgtga tgacgaaaat tcatcagatg
     1021 actgcgacgc ctgtttcaga cgggcattta cggaactcca ctcatcagcc agtgaaatca
     1081 ggtgtccggt ggcaaaggca ccggcaaatg cgccagccat tccgacagcc gaagcgcgga
     1141 tttccgtcaa ctggctgttc agttctgcca gggcacgtcg ctgctcccgg gctgccgcag
     1201 cggcctgacg cccgccattc tgcagggtcc ggtaatattc actgcccata cgggaagccc
     1261 gctggatctc cgactggaat gactg
//
LOCUS       DAZFBR010000343         1277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFBR010000343 DAZFBR010000000
VERSION     DAZFBR010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.346"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..153)
                     /locus_tag="ACHJZ2_005295"
     CDS             complement(<1..153)
                     /locus_tag="ACHJZ2_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4571170.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAG"
     gene            complement(378..932)
                     /locus_tag="ACHJZ2_005296"
     CDS             complement(378..932)
                     /locus_tag="ACHJZ2_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO4571171.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(995..>1277)
                     /locus_tag="ACHJZ2_005297"
     CDS             complement(995..>1277)
                     /locus_tag="ACHJZ2_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4571172.1"
                     /translation="YPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSG
                     RLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRG"
BASE COUNT          309 a          359 c          250 g          359 t
ORIGIN      
        1 acccgccata accagctggg tcagtattaa ctggcagcgt tcgcgtgaaa ggtaagtatt
       61 ctgcgcaatc tccccgactg tcgccggttc ggtaacgctt aattcattaa acaccactct
      121 ggcggtttct gtcatatcct gctgttttag catgtctttt tcccttttcc ggttaacgtg
      181 acataccaat aactcttgtc gaaaaagcca gcaagttgaa agaccggtat tagcaaccac
      241 cagcgcgttt aacgccccgt gccgtttttc agtcataaaa aaacccgcaa aaagcgggct
      301 ctttcaaatg tccatgtctg ctattcgcct cgcggtacag ctttgcgaag cttaccggaa
      361 ttgaagcagt ttttacgtca aaaagcaata acttttttct ctataccaaa agccataacc
      421 attggtttgt acaaaataaa ttctgccacc tttagccaat gctcaatgcg tctttcacag
      481 gttcttaaac tccattccgg atgtgcatca ttcagcaatt cagccatttt gcgcttagtc
      541 atcccccgcc ccacataacg ctgactcaga acattgagca gcccgggata acctgccagt
      601 acttcaccaa taaccctatc gattattaac gcctctgaat cggtacaatg tgccagccag
      661 cttttttgat tgccgttgat catatcccgc aaaaaagcct caagttcagg tttgtccaga
      721 cccgcttttt tcatcctccg gagcgcctcg ttaattgccg tttttgtcag ctttttagag
      781 gtcagtaatt ggttgaacat atttcccgtc ttaccgtcgc caatatacga ccaacgcccc
      841 cacatacgca gtttcccctg gatccagaca ctttccagcg ttttcaggcg taaatgctca
      901 ccgcttttgc ctgtaatttc cgggtatatc atatttatgc tcactcactt tcaattttgt
      961 aaatcttcac gcccagccgc ccaccagaaa cgagctagcc gcgcacaata ttgatttcat
     1021 caaactgctc gtcgtctatg agaagtccgg catgcgtcag cgcatccagt ggtgctttca
     1081 ggatattgtc caggtcacga cggcgcttat ccggtggctc tgcaataatc tttatcgcca
     1141 gccttccgga caggtttaat ttcagccgct gctggcgaac aataagtgcc acatcccggc
     1201 gataacgctc accggctttt gatacaaaat atgtgctgcc acgacgtcgc cagtaggtgt
     1261 tcaccgtcgg cgggtaa
//
LOCUS       DAZFBR010000344         1270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFBR010000344 DAZFBR010000000
VERSION     DAZFBR010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.347"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..79)
                     /locus_tag="ACHJZ2_005298"
     CDS             complement(<1..79)
                     /locus_tag="ACHJZ2_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4571173.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTS"
     gene            complement(91..489)
                     /locus_tag="ACHJZ2_005299"
     CDS             complement(91..489)
                     /locus_tag="ACHJZ2_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO4571174.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(531..>1270)
                     /locus_tag="ACHJZ2_005300"
     CDS             complement(531..>1270)
                     /locus_tag="ACHJZ2_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4571175.1"
                     /translation="DPQNLADPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKY
                     TMTGEAFDPVEVDMGRSAANNITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNII
                     VFDPKGWALFRSFKAVREKLDTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQ
                     YVENGVKKNFLPDNTMVLGNTQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDP
                     AREFTMIQSAPLMLLADPDEFVSVQLA"
BASE COUNT          248 a          399 c          333 g          290 t
ORIGIN      
        1 ctgacgttcc catgtacccg cgtatcgttt catcggcgcg ggcaatggca gcatcgaaca
       61 ggttatcgaa atcagccaca gcgcctcccg ttattgcatt ctggccaggc cgcgctctgt
      121 catttcggct gccacaccgg cagagacacg aaacgccgtt cccggcagca caaatgccac
      181 aggttcatcc cgcgtgacgt gaagtgcatc agtatgcagc ttcaccagtg ccacgaccgt
      241 gaccagttca gacgtatcca gaatcacggt atccggctgc gctgatccca cctcattttc
      301 atgtccggtc agcacatttt cccggctgag aggggtgtcc tgaccggcag tttcatccgt
      361 gtcatcaagc tcctcttcca gctctgccac acggagcgcc agttcttctt tcgtccccgt
      421 caggctgaca tcacggttca gttgttcacc cagcgagcgg agacgggcaa tcagttcatc
      481 tttcgtcatg gactcctcca cagaaaaaca atggccccga agggccatga ttacgccagt
      541 tgtacggaca cgaactcatc agggtcagcc agcagcatca gcggtgctga ctgaatcatg
      601 gtgaactcac gcgccggatc gccggtggtc acccagtttt tcgggtaacg ggcagaggcg
      661 ttaatgcctt cgcgctgtgc gtccgcatcc tgaatgcagc cataggtgcg cagaccgcgt
      721 gcctgagtgt tccccagcac catcgtgttg tccggcagga agttcttttt gacgccgttt
      781 tccacgtact gtccggaata cacgacgatg gccacatcgc catacattcc cttataagac
      841 accgctttgc ccaggtcttt taccgctgtc tccagttcgg aatgagagcc gcgacgggta
      901 tccagcttct ccctgacggc tttgaaggaa cggaacagcg cccagccttt cgggtcaaac
      961 acgatgatat tcaccacgcc gctggcgttc agcgcgtagg cttcgatatc gtcggtcggg
     1021 tcatacgtgg acttgtcacg cttgctccac tccgtgccgc cggactgcgt gatgttgttc
     1081 gccgcactgc ggcccatatc cacctcaacc ggatcgaagg cttcaccggt catggtgtat
     1141 ttgcccttga gcacggcaga aactgcctgc atctcttcga cctgagcaat ggccagctct
     1201 tcgtcacgca tgttctgcat gatgatgcga cggcggcggt aagccgggtc cgccagattc
     1261 tgcggatctt
//
LOCUS       DAZFBR010000345         1253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFBR010000345 DAZFBR010000000
VERSION     DAZFBR010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.348"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..390
                     /locus_tag="ACHJZ2_005301"
     CDS             <1..390
                     /locus_tag="ACHJZ2_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO4571176.1"
                     /translation="ITFVAKMPYIDSIKLLEAGAKGCVWKTSHPAKLNRAIDSISNGY
                     TYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIADGKTNKEIANFLQLSRKTVETHR
                     LNIMKKLDVHSGIELIKTALRMGVCTI"
     gene            complement(409..912)
                     /locus_tag="ACHJZ2_005302"
     CDS             complement(409..912)
                     /locus_tag="ACHJZ2_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO4571177.1"
                     /translation="MRSPKVKFLTIFTLSILITKMSFASSACFNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKKNNIKSFDYGLMQINQMHIPMLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYKHFSRCGMTWQCLGTYNAGFAMDNQKKRLQYAKKIYIVYTRLNELD
                     NRKALAK"
     gene            complement(997..1215)
                     /locus_tag="ACHJZ2_005303"
     CDS             complement(997..1215)
                     /locus_tag="ACHJZ2_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571178.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDKVQIQAF
                     IVSTWMASFQNDMYSEDNPISPYHKIEW"
BASE COUNT          391 a          212 c          218 g          432 t
ORIGIN      
        1 attacatttg tagcaaaaat gccgtatatt gattcaataa aattgcttga agcaggtgcg
       61 aaaggatgcg tatggaaaac cagtcacccg gcgaaactta atcgcgctat tgattcgatt
      121 agtaacggct acacttattt tgatagtgta catatggatt gtgagaaaat atcttccaga
      181 tattcctctg ataatcaact tacaaatcgt gagtctgaaa tattacaact catagctgat
      241 ggaaaaacaa acaaagaaat cgctaatttc ttgcagttaa gcaggaaaac agtcgaaact
      301 catagactca atatcatgaa gaaattagat gttcacagtg gtatcgagtt gatcaaaaca
      361 gctttacgta tgggtgtgtg cactatataa aaaaaagagg ctttaggctc attttgctaa
      421 agccttacga ttgtctagtt cattaagcct ggtatataca atatatattt ttttggcata
      481 ttgcagtcgc tttttttggt tatccattgc aaaacccgca ttataagtcc ccaggcattg
      541 ccacgtcatc ccacaacggg aaaaatgctt atataaaatc tcggttccta ttttaatgtt
      601 caggcaggga ttatctaaca aatctcgttc atcttttatt attccacgtt tttttaacat
      661 gggaatgtgc atctggttta tctgcattaa accataatca aaactcttta tattgttttt
      721 cttatctctg ttcttcccta tgctatcctt ttttagattg ctttcaacaa gagcgatagc
      781 tttaatcaaa tttggttcga ttctgaacat tgttcctgct tcattaaagc aagcgctact
      841 ggcgaaagac atttttgtaa ttaatataga aagcgtgaag atggttaaga atttaacttt
      901 tggtgagcgc atatatcttt atgagtcagg attgtataaa aggaacgaga tactattata
      961 aacagtatct cgttaataat ttatttggtc aatcgtctac cattcaattt tatggtaagg
     1021 tgagatagga ttatcttctg aatacatatc attttgaaaa gacgccatcc atgttgaaac
     1081 tataaatgct tgtatttgaa ctttatcagt agcactggtg ttgatgtgtt tatctaatga
     1141 aacgacttca tcacccgttg cattcgctat atttgaactt tgagataaat tattgatacc
     1201 gattggatta gtcataaagc tcccttttta attgtccagg ccaatggtct tta
//
LOCUS       DAZFBR010000346         1233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFBR010000346 DAZFBR010000000
VERSION     DAZFBR010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.349"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..676
                     /locus_tag="ACHJZ2_005304"
     CDS             <1..676
                     /locus_tag="ACHJZ2_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571179.1"
                     /translation="IESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAG
                     RINARLEDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVC
                     RWFGTSDLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEI
                     DAFFCAFREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALRE
                     ELETML"
     gene            673..>1233
                     /locus_tag="ACHJZ2_005305"
     CDS             673..>1233
                     /locus_tag="ACHJZ2_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO4571180.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGA"
BASE COUNT          309 a          305 c          357 g          262 t
ORIGIN      
        1 aatcgaatcg accgtgacca gcctgcaccg tgaggctgaa gcacagttcc ggcctgagct
       61 ggagaaaatc gtcagcggga ttgaaacagg ttttcgtggc acggccctgt acgccacaga
      121 aaacattgcc ggtcgcatca atgcccgcct ggaggatgag ggctttaccg taaaaatcag
      181 ttttccggca gtcagccagt tacagacccg gctcgcggta aaaataaatc tgagtgcgct
      241 tatggaggaa agaacggaga ccgtcacccg tcgccgtcgg cagagtggcg tatggggaac
      301 cgtttgtcga tggtttggca ccagtgacct gggctgggaa aactatgacg aggatgtgag
      361 tcgcagcgtg atcaatatca acaaggtcag agaggaagtt atgtcactga cccgggcata
      421 tttcggggag ctgcaggcat ccattgagca ggatattaac cagcccgtcc gccaggaaat
      481 cgatgccttt ttctgcgcat tcagggagaa agttgaacaa ctgcgtaaca cgctgattca
      541 gagctctgaa gatcataaac gcgatcagca ggcgcaggaa cggcttaccg ggcgacttca
      601 ggcattaaat gaaagggttc ctgagctcat tactgacagt aaggcgctga gggaagagct
      661 ggagacaatg ctgtgacttc accatttatt caacaaattg ctgataaccg ggtatgtcag
      721 gtactcacct gtcttcctga aaaatttgtg gttgatttcg caaacggaat cgatgttgca
      781 caggagcata tccgcacggc cggagagcgc acgttcttcc ggcgtttaaa agaggggctg
      841 accggcgaag gcgctgcacg gcagaatgcc atcaatgcgt cgcttgcaca gggcgttgag
      901 gcgtccctgc gctggctgac ggagatgaca acgtcacttg ccaccacaaa ctacgcgatt
      961 acccgggtaa acgacagggt cagttcactg gtcagtgata cagccaggct ggcgcattat
     1021 tccgcagata cgcgggagca gttactcacc ctggccgatc aggttcacca taaactgaat
     1081 catcttgaag aaaaactcca ccgtgttgac caggttcagc gggcgcagtt acatcttgag
     1141 cagatattct catggtggag cgccgggcga tatgcgtcat tttcccctgc cggacgttgt
     1201 tatgtggcac ttgaagagct tcgctggggc gcg
//
LOCUS       DAZFBR010000347         1221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFBR010000347 DAZFBR010000000
VERSION     DAZFBR010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.350"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..519)
                     /locus_tag="ACHJZ2_005306"
     CDS             complement(<1..519)
                     /locus_tag="ACHJZ2_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4571181.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVHAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIG
                     QEAVEEIESNHNR"
     gene            complement(494..1060)
                     /locus_tag="ACHJZ2_005307"
     CDS             complement(494..1060)
                     /locus_tag="ACHJZ2_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4571182.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGAFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          287 a          293 c          233 g          408 t
ORIGIN      
        1 gcggttatgg tttgattcga tttcctctac tgcttcctgc ccgatgcggc taatcagttc
       61 gacgcgatac ggaacgagat ttccgctttt gtgctggttg cacaccacgc attgcttgtg
      121 aatattgcgt tcatcaaatc ggagttgagg tgccgcagca gttgtccggt aatgtccggc
      181 atcccactga gcagacgtga gcgttccgca cgagatacat ggtaagtcgc ggtctctttc
      241 tctgatgaag gcgtgtacgg cttgttgggc ttgtttaatc cagtaactgc ggggctttaa
      301 ggcgagtttt tgaatcttaa gtttatcttt ctgtttctgc tcctctcgtc gtcgtttctt
      361 ctctgctgcc ttttctgcct tttccgcttt ttcgcgttct ttacttcgtc gttcgagtgc
      421 tatcttggtt ccacaaatct cattacacca atattgattt tgatattttg gtataaacca
      481 ttcattgcaa catttacatt tccttcgata gattcgcata agtgctcctt tcgttgccgg
      541 aaaaatcacc gtaatactta tctcgggctt cttcagcaac tagtaccgct aactccagat
      601 catcaaagca tccgaagtgt ttactcttgc catggaatcc tagcctaaca ttccattttt
      661 tctgtcgttt gtgccaagta acccctctgc aacctgattt gctattcttt cggatcctta
      721 tatttcttga attttctatt ggcaggcatt ctcttaaatt ttctggccta ttgtcggtcc
      781 taattccatt aacgtggtca atttgaccag caggccaacg attatgagtt atgtaaaaaa
      841 ctaagacgtg agttttatat ctacgcccat ctatcatgat cattgaataa ccgttggaat
      901 caaaagctcc agcaacacta tttaatgcta tccttccctg agtgggaact ttccatctaa
      961 ataccccggt agatttatcg aaacttagta actcaaatat ccttttaaca gttaaatctt
     1021 ctcttttacg gttacatcgt cttcgcgctg gtttagccat cgccttcttc ctccgtaatg
     1081 gttttctgaa tttggccacc tgaacagagc tcaccaaagc tatggatgtc ggtatttcca
     1141 caataccaag atggcgaaaa taactgcatg ataagcctca gggaaaaggg aagacactac
     1201 ccccgataat tcagaaacaa a
//
LOCUS       DAZFBR010000348         1203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFBR010000348 DAZFBR010000000
VERSION     DAZFBR010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.351"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            77..727
                     /locus_tag="ACHJZ2_005308"
     CDS             77..727
                     /locus_tag="ACHJZ2_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4571183.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            806..>1203
                     /gene="espW"
                     /locus_tag="ACHJZ2_005309"
     CDS             806..>1203
                     /gene="espW"
                     /locus_tag="ACHJZ2_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO4571184.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNPVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRIDID
                     SLFLKKLNNVRNQNKFPIDVKETVIKLQGQ"
BASE COUNT          414 a          239 c          217 g          333 t
ORIGIN      
        1 tattcacata tcatgagcgg catgttgatt cttgttacca atatagttca caatatggat
       61 taaaaaggat aataatatgc cagtcatatt aaatttttct aatggaagtg tattgccgga
      121 aaatgagctg gaggctttac gacatatagc aagaagcaac cagaatgaca ctattactat
      181 aggaggccgt aatatgaggc ttcattatat ccagtttatg gatggtttta gtgttgagcc
      241 tattcttggt ggactttggg accatcttgg ggcaagagag gctcatcatc tcgcagaccg
      301 cctgacaaga caacttaacg gaggtaacac ttttctgcag gcatacagct tatatctgga
      361 gcagaggcaa gctgccccgc ttgtgcagga gagcgtcata aaaacactac tagatcgaat
      421 aaattcgaat gcatttcccg ttagtttaca agacttttcc tgtactgagg aacatcttaa
      481 ttgtccgata acgctacata ttcctgagac aggtgttttt gtcagaaatg ctcgaaattc
      541 agaaatatgt gcattatatg atcaggaagc attgactgaa cttatcctgc gtaacgctct
      601 ccaccccctc agccgtgacc cctttgctcc agaaatgatt ataagcaaag acaagtgtca
      661 ttttaatata acaaaacaat gtttttacgc attacccata tacccacttc aacaaaacag
      721 tatttaaaaa taaaagcaca atataagaag catattttca ataagttaat caccatataa
      781 caaacatata gcgaggagac tatttatgcc caaaatatca tcagttgtat catcatgtta
      841 ccatctgttc agtgaacatc aacaactttc aaatgaaaca acaatgacga accccgtctc
      901 cagaagaatt gttcataaag aatatggtat atctttaaaa tccgttcctg tatggttggc
      961 tacagctaaa actccccttg ctctactcaa tggcagacat acaagaagtc actcatttat
     1021 tattgcaggg accccaggaa tgggaagcag gagcggagcc caatactatg ccataaacag
     1081 tgatgataaa cgctcccgaa tagacattga ctctttattt ttaaaaaagt taaataatgt
     1141 gcgaaatcaa aataaatttc caatcgatgt aaaagaaacg gttataaaac tacaagggca
     1201 aaa
//
LOCUS       DAZFBR010000349         1202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFBR010000349 DAZFBR010000000
VERSION     DAZFBR010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.352"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..814)
                     /locus_tag="ACHJZ2_005310"
     CDS             complement(<1..814)
                     /locus_tag="ACHJZ2_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4571185.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSP"
     gene            complement(760..981)
                     /locus_tag="ACHJZ2_005311"
     CDS             complement(760..981)
                     /locus_tag="ACHJZ2_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571186.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(1026..>1202)
                     /locus_tag="ACHJZ2_005312"
     CDS             complement(1026..>1202)
                     /locus_tag="ACHJZ2_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571187.1"
                     /translation="MQSEPGGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAV
                     AVITGVNYGSASGG"
BASE COUNT          230 a          399 c          310 g          263 t
ORIGIN      
        1 ccggtgaaaa cgtcgtgggg ttgtattttg ccccgttgct cagaatggcc gttttccccg
       61 cattttcgcc tgtatacccg ctgtcccagt tgctcttcag ttttttcgca ttttcttccg
      121 taatactgcc ggggatctca atcaccccgg acggcctgcc gccatttctg aaaaaagacg
      181 tcgaattttc ctgaatatga tgcccctgcg tggccgccag cccggcggca tacaccggcg
      241 gcaatcctat aagcggatga aaaaaacagt taaaccggtc gtggatcact tcccgggcag
      301 gcaccgtcac cgcctccgtg atcccgcagt tccggtccgg cgtgatgcgg tagaacacct
      361 cgccgtcatc cgccaccaga ggttcaaccc ggctccagtc cagaatacgc agttctttga
      421 tctgcccccg ggcattacgg attttcagca ccaccgtatt gccatgacgc agtttggcgt
      481 tcagccacag ttcaaaaaac tggatgcggt tctgctgggc gttgggacga cgacagaggc
      541 gggcaatatc cccccggcgc gtttccctgc gtatcccatg cgcatccgtc tgcataagac
      601 gcagccgcat tttggcgata tcctgggata tcagcgaaat acatgcaaac accgcatgaa
      661 aggagaggac ggcttcagga tcggctttca cgccctgctg ccaggcgccg gaaaagggct
      721 cagccaccgc ctgaaacagg ctggtccagc ccgcctctct tacgtcacgt cctgatttct
      781 ggttttttcg ggttcgccgt aaaaggttcc acattcgcca tgctccgcat cacgtttctt
      841 tttctgacct gccggacgtc gcaccgtgat gtactccgcc tttcccaggc gaaccagcac
      901 ctccgcacac ggctgtgcca catcacggat atccccggcc cgggcatcat gcgtgccctg
      961 cagatatcgg atctttgcca taacctgtta cgggaggcgc acgcctcccg tcctccttat
     1021 cagactcagc cgccggacgc actgccgtag ttcactccgg tgatcaccgc caccgccgca
     1081 gtacggcgac gacgccagtt gatccagcgc tccgcacgga tggccacgct gcctgtctgg
     1141 aacatggaaa ccagctccac cggggacggc gtggtactgt cgccgcccgg ctcagactgc
     1201 at
//
LOCUS       DAZFBR010000350         1201 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFBR010000350 DAZFBR010000000
VERSION     DAZFBR010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.353"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            56..646
                     /gene="espM2"
                     /locus_tag="ACHJZ2_005313"
     CDS             56..646
                     /gene="espM2"
                     /locus_tag="ACHJZ2_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFO4571188.1"
                     /translation="MPMNTTGMSFSSFGISCHRENSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSGNILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFFLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPDYLMRAIQG"
     gene            880..1032
                     /locus_tag="ACHJZ2_005314"
                     /pseudo
     CDS             880..1032
                     /locus_tag="ACHJZ2_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          389 a          218 c          277 g          317 t
ORIGIN      
        1 acaataaacg caagtatgca atattgtaaa cactttttta taaaaaggaa ttataatgcc
       61 gatgaatact acaggtatgt ctttcagctc ttttggtata agttgccata gagagaactc
      121 cttcaggaac tctttccgag ggaagaatga tgaggtcgta aaatgttcaa ttggagaacg
      181 gactattagc ttttctgttc gcaaatttag cggcaatata ctggaaacgg tgagaaggca
      241 gagtactaaa gatattgatg agtggataaa agatgaacgg atagtatatc cctcaagggt
      301 gatcaaccag gagattgata attactgttt tcaaaaaaat gcaaaaattt ccactgaaga
      361 gaggcaaaga gtcttttttc ttgtgagcca ggagaatcaa ctaacccttg atgttaaggc
      421 agcacaaagt tctattaatc atgtaataat ggggagtgct tcttttggca agaaaatgga
      481 tgctctttgt gatggtatga gccgggatgt aaaaaatcgt acatcagata ccatagcaaa
      541 cttgcttgcg gacaagtttt accagaaaca tatcgattcg gatattgata ttgtaaaact
      601 acgaaatgat atcccagatt atttgatgcg tgctatacag ggatgaggct agaggagagg
      661 actgcttcag catcatcggc ttcggaccag taaaagagac gcagaaaatg cacaacggca
      721 ccacacgcca tgcatggatt cagattactc atggctcacc agtacagcta taaatcgtaa
      781 agaaaactac agtacgttat acacagaaac gtaccgtgat tcattattta ttgtacaggt
      841 tcatcgcagc gacaatattt tgtgatgttt gcgtggtgcg ccccacttca ggatgctgca
      901 gatctggaaa ttgcaacgga ggaagaaacc tcattgctgg aagcctagaa aaagtatcgg
      961 gtgttgctga accgtgttga tacatcaact gcacctgata ttgagtggcc ggaagaacca
     1021 gacacaatgt aagcgaaaaa gaaaaaccgc agacacgacg tatgcaggac gtgctgcggt
     1081 tggctggtga actttcgata gtgcgagtat tgaatgattt ccagccgtta ccgattttac
     1141 gtgttaatta gtgaacaaac cactcgtcag cagacttcca ggtatctttc agagtctcct
     1201 g
//
LOCUS       DAZFBR010000351         1201 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFBR010000351 DAZFBR010000000
VERSION     DAZFBR010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.354"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..330)
                     /locus_tag="ACHJZ2_005315"
     CDS             complement(<1..330)
                     /locus_tag="ACHJZ2_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO4571189.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKN"
     gene            complement(965..>1201)
                     /locus_tag="ACHJZ2_005316"
     CDS             complement(965..>1201)
                     /locus_tag="ACHJZ2_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO4571190.1"
                     /translation="KQLRYLEKSASQGNVTAQFNYGVFLSDTNPTLSEYYNLNRAIYW
                     MEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          353 a          250 c          199 g          399 t
ORIGIN      
        1 atttttcaga tatggtctta tgatctttcc cttacttaat aaatagcatg tataggattc
       61 ggaaagtcca gcatctattt tgtatctcat tatattagat aaggatgtga gaaattcctt
      121 gttatcattc gcagccatat atgccagtcc gagttcagcc tctgcggcct tacaggcaaa
      181 atagcttagt gaacacgctt ttcgtccctc tttagtactg gctgatatta gtgcccataa
      241 tattgctttg tctgccaaat ctaactctct ctgaaattgt cttgcagtaa ctgcactcgc
      301 agaaatagag aaaaaaatac aattgttaat aaccttgcca tattttttag tccgtaactg
      361 ttaatgtgct atctggctct gaaaatatta actcatttaa tttttgcatg tcattagata
      421 aaccagttat acaaccctcg gaacagaatc gaggtcctaa agacggacca aaagcgttta
      481 aacaactatg tattgaaaat ccattgcgat tataagttcc tgtattaact gacggtacta
      541 atcgtatgcc gtcaggtttt ctattgcttg ctcccgggtc attaacattc caactccata
      601 ggccttgaga tattggccat ctatttataa atgtgtacaa cggggattat ttttacattg
      661 catattatta ccagaagcga ctggaatact aagcacatca taaaaattat catcacatgg
      721 tatatctgaa acacacgaaa gcatacctaa agttatgctg tataaacagg tggctaatcc
      781 caaggggaac gattccagaa atcgggttca acggatactg ataaaaattc cacctacctt
      841 ccagcccaat cggagcctga gtgttatacc gcccctgctg cggttaatgt taccgataat
      901 agttatagta cagccctgat tcatcatcat actactgctg cggcagtctg atgaactatt
      961 gcttttacgg attctcctta ttcttccttc tatccattcg ctttagcttt ttgagctctt
     1021 gaagtttgct ctttgcatca atattaccgt tatttacagc gaattccatc caataaatag
     1081 ctctattcaa attataatat tctgataatg ttggatttgt atctgataaa aagaccccat
     1141 aattaaactg cgctgtaaca ttcccctgag atgctgattt ttctaagtat cgcaattgtt
     1201 t
//
LOCUS       DAZFBR010000352         1188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFBR010000352 DAZFBR010000000
VERSION     DAZFBR010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.355"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(1..136)
                     /locus_tag="ACHJZ2_005317"
                     /pseudo
     CDS             complement(1..136)
                     /locus_tag="ACHJZ2_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            286..954
                     /locus_tag="ACHJZ2_005318"
     CDS             286..954
                     /locus_tag="ACHJZ2_005318"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4571191.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          357 a          250 c          223 g          358 t
ORIGIN      
        1 ctactgatgt atcaacacga ttcaacaata cccgatattt attccatgcc tccagcaacg
       61 atctttcttc ctccgttgcg atttccagat ctacagcatc ctgcagtggc gcaatatact
      121 cactgaattc ctggatgtag aactgtgtgg tgacggtctt ccagccattc ggctcctgct
      181 gtatcgcagc ataccaggct atttcaatat cgctatgctg cggcagcatt taaccccttg
      241 taattcatcg ccataattga tttaattcac aaataaaact ataacatggt gaaattaatg
      301 aaaaaaaaca cagatgatgg ggctaaaatt tacacaccac ttaccctaaa gctttatgac
      361 tggtgggttt tgggagtatc aaatcgtctt gcatggggat gtcctacaaa ggaacacctt
      421 cttccacact ttctggaaca tttaggtaac aaccatctgg atattggtgt tggaactggg
      481 ttttacctta ctcacgtacc tgagagtagt ctgatatctt taatggattt gaacgaagct
      541 agcctgaacg cggcatctac aagggctggg gaatcaaaaa ttaaacataa aattagccat
      601 gatgtttttg aaccttatcc cgcggcgtta catggtcaat ttgattccat ttccatgttt
      661 taccttcttc actgcctgcc tggaaatata tctacaaaaa gctgtgtaat acgcaatgcg
      721 gcgcaggcct taactgacga tggaactcta tacggagcca caattcttgg cgatggagtt
      781 gtgcacaata gcttcggtca aaaactgatg cgcatttaca atcagaaagg catcttttca
      841 aacacaaaag attccgaaga aggcttaaca catatactct cagagcattt cgagaatgtt
      901 aaaaccaagg ttcaaggtac tgtagtaatg ttttccgctt cagggaaaaa atagcatcca
      961 accgcagcac gttcttgcat acgccgtgtc tgcggtttaa tttttactct caaatattgt
     1021 agttaagtac aataatgatt cctgtggcta tatagatgtt tttgtaatta gtccttaaga
     1081 taaaatttct ctatttatcc aagaaacaga cttcattagg aagagtcgta aaaacattaa
     1141 caactaccgc cgtgtaatta aattattttt tagaatgttc tgattgag
//
LOCUS       DAZFBR010000353         1183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFBR010000353 DAZFBR010000000
VERSION     DAZFBR010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.356"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            183..446
                     /gene="ykgM"
                     /locus_tag="ACHJZ2_005319"
     CDS             183..446
                     /gene="ykgM"
                     /locus_tag="ACHJZ2_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO4571192.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            446..586
                     /gene="ykgO"
                     /locus_tag="ACHJZ2_005320"
     CDS             446..586
                     /gene="ykgO"
                     /locus_tag="ACHJZ2_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO4571193.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(656..847)
                     /locus_tag="ACHJZ2_005321"
     CDS             complement(656..847)
                     /locus_tag="ACHJZ2_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571194.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
BASE COUNT          325 a          265 c          257 g          336 t
ORIGIN      
        1 tgtacgctta ccaatcactc atccaggcgt ttatgggagc gtcgccaatg gctgttaaca
       61 atgccagact tccccgttga ggaaattcca cataccacta atggtcacag ggattgggtg
      121 ttgaaatgat ccggatgagc atgtatcttt gtggttatgt tataacataa caggtaaaaa
      181 tgatgaagcc caatatccat cctgagtatc gtactgtggt gttccacgac accagtgttg
      241 atgagtactt taaaatcggc tcgactatca aaacagaccg tgagattgag ctggatggcg
      301 taacgtatcc gtacgtaaca attgatgtct cttcgaaatc gcacccgttc tatacaggga
      361 agctgagaac agtggcatca gaaggaaatg ttgcacgatt cacccaacgt tttggtcgtt
      421 ttgttagcac gaaaaagggg gcgtgatgaa agtccttaac tctctgcgta ccgcaaaaga
      481 acgccatcca gactgtcaga ttgtgaagcg aaaaggacgg ctatatgtga tttgtaaatc
      541 taatccacgc tttaaggccg ttcagggtcg taagaaaaaa cgttgattta aaattcgacg
      601 aattaacgat atttgtctga ttaataatca gaccggatta atgttggtgt atttattaca
      661 ccagcattaa ttttcctggg gatgtatctt cctgttcatt taaggtcaac tgcctgacgt
      721 ttctctccga atattccatt atattaatgt tgacttgttg accagcttcg cccctgtatg
      781 ctggcatcaa ccctctttta gactgaacac gccactcagt ttcctccctt tgcgacgcag
      841 cctgcattta cactcaaact gttaagatga taaatgtggt aaatctgttg gtactgacat
      901 aaaaacgttt acgccacagg aacggcctga tccaccggta accccgtcgc cgacgttcga
      961 gtgccagtta gagtaacgcg cacagatgac tgaatgcagt gccctggcaa agaggccatc
     1021 gttccggtga caactggcag tcttcgttta acttcactta atctggctct tagggggctt
     1081 accggacaga tgacgtactt acacctgttt aatttttcat cacttattgg gatgaacacc
     1141 cataaccatt ttgtgcggca tgttaatcca ttaaaacacc tta
//
LOCUS       DAZFBR010000354         1180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFBR010000354 DAZFBR010000000
VERSION     DAZFBR010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.357"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..253
                     /locus_tag="ACHJZ2_005322"
     CDS             <1..253
                     /locus_tag="ACHJZ2_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4571195.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAVNRRR"
     gene            268..801
                     /locus_tag="ACHJZ2_005323"
     CDS             268..801
                     /locus_tag="ACHJZ2_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4571196.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            798..>1180
                     /locus_tag="ACHJZ2_005324"
     CDS             798..>1180
                     /locus_tag="ACHJZ2_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4571197.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          280 a          274 c          359 g          267 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggtgacacgc tgaccattaa cggcgagatg ttctgggtgg accgtgtttc
      181 tccggatgac ggagggagtt gttatctctg gctcaaccgt gggcaaccac cggcagttaa
      241 ccggcgacga taaacgcagg gtgaattatg gcgataaaag ggcttgatca ggcgattgaa
      301 aatctgagcc gggttcgtaa aaacgccatt ccggcggctt cagcaatggc cattaaccgc
      361 gtggccacaa cggcgattaa tcagtcttca tcacaggttg cccgggagac aaaggtacgc
      421 cggaaactgg taaaggaacg gtccagactg aaacgggcca cggtcagaaa tccgaatgcc
      481 agaattatcg ttaaacgcgg tgatctccct gtgattaagc tggggatcag gatgctgggc
      541 cgtcgtccgg acagcatact taaagctggt cagcatcggt atcagcgggc atttatccag
      601 cgattaaaaa atggtcgctg gcatgtcatg cagcgtgtgg ccgggaaaaa ccgttacccc
      661 attgatgtgg tgaaaatccc gatggcggcc ccactgaaac aggcgtttga tgagaatatt
      721 gaccgtatcc ggcgtgaacg tctgcccgga gaactggcat acgcgctgaa acaacaactt
      781 aggattgcga taaaacgatg aaacatactg atatccgtgc ggcagtgctg gatgcactgg
      841 agctgcatga acacggggcg acgctgtttg atggtcgccc cgttgttttt gacgaagagg
      901 attttccggc cgtcgcggtt tatctgacgg atgcagagta taccggtgaa gagctggatg
      961 cagatacctg gcgggccaca ctgcatattg aggtgttttt accggcacag gtaccggatt
     1021 cggagcttga tcagtggatg gaaagccgga tttatccggc ggtgactgcg atcccggcac
     1081 tggcagacct gattacgacg atggttacgc agggctatga gtatcgtcgt gatgacgata
     1141 tggcgttatg gagttctgcg gatctgactt attccattac
//
LOCUS       DAZFBR010000355         1170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFBR010000355 DAZFBR010000000
VERSION     DAZFBR010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.358"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            360..>1170
                     /locus_tag="ACHJZ2_005325"
     CDS             360..>1170
                     /locus_tag="ACHJZ2_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571198.1"
                     /translation="MTIAKDSSTDEIIHGNDLRGMDDFYIKTTSFECPYEPCKIKATP
                     CSFTMKHVNQSYFRYGDKHKDGCGIHDPRYKNNHTSNDERKHNSPPAPVISLLKIDVK
                     PRGGVKNARNIKNENHKDEKKGNEHPVSSSSIKPVVDYYINNSNHNEQLSIPPYGTRS
                     YKDTFQLIFYKNNIRYYKPAIYYGVAQSNIRLHEDSDKHCITFLARDKKTQKPFTLEI
                     DVSDWNKSQKDVFWKEYEKQRKEADRYYKGLKDKRNAKKYLTVFFFGMPDEN"
BASE COUNT          427 a          181 c          204 g          358 t
ORIGIN      
        1 agtctaaatt ttaatattag attcgtaaat ctataattcc atgaatctaa ttaatgctta
       61 aaagcatcct ctaagatgct tttactgtta ccatgcatac atacccagaa cctttaacaa
      121 acgataatag aggggagttg tgtataacaa ctccctgacc ttactaaggt tagcgatgtt
      181 attcagcatc attctgtcaa agatttaatt tctttgaaaa atactaattc taccccttaa
      241 gttaataact tctgcttgat cgtgtatttt ggtaagttgg agtacaattt tttgattgga
      301 tcgtcaataa gaaaaagcta gaatccttag aaggacatag attttcatag ggttaagata
      361 tgacgattgc gaaggatagt tcgactgatg aaatcattca tggaaatgat ctccgtggaa
      421 tggatgattt ttacattaag acaacgagtt ttgaatgccc atacgagcct tgtaaaatta
      481 aagccacacc ttgtagtttt actatgaaac atgttaatca atcatatttc agatatggcg
      541 ataaacataa ggatggatgt ggtattcatg atccaagata taaaaacaat catacttcaa
      601 atgacgaaag aaaacataat tcgccacctg ctccggttat atcactttta aaaattgatg
      661 tgaaaccaag aggtggtgtt aaaaatgcca gaaatattaa aaatgaaaat cacaaggatg
      721 aaaaaaaagg taatgaacat cctgtatctt catcaagtat taagccagtc gttgactatt
      781 acataaataa tagcaatcat aatgaacagc tatcaatccc tccatatggc acgcgatctt
      841 ataaagatac atttcaactt attttttaca aaaacaacat acgttattac aaacctgcta
      901 tttattatgg tgtagcccag tctaatattc gtctgcatga agattcagat aaacattgta
      961 ttactttcct cgcaagggat aagaaaaccc aaaaaccatt tacgcttgaa attgatgttt
     1021 ctgactggaa taaaagtcag aaggatgtgt tttggaagga atatgaaaag caaaggaagg
     1081 aagctgatag gtattataag gggcttaaag ataaaaggaa tgctaaaaaa tacttgacgg
     1141 ttttcttttt tgggatgccg gatgaaaatg
//
LOCUS       DAZFBR010000356         1162 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFBR010000356 DAZFBR010000000
VERSION     DAZFBR010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.359"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..1104
                     /gene="ehxD"
                     /locus_tag="ACHJZ2_005326"
     CDS             <1..1104
                     /gene="ehxD"
                     /locus_tag="ACHJZ2_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO4571199.1"
                     /translation="KDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAIL
                     SESIQRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKI
                     VERDAALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVW
                     LAQVSQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSF
                     IKAPVSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVV
                     IKVDAYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAG
                     MTVMAEIKTGVRSVISYLLSPLKETINESLRER"
BASE COUNT          419 a          171 c          247 g          325 t
ORIGIN      
        1 aaagatggct cttttgtaaa aaaaaatgat ccattagttg aattgacggt gcctggtgtt
       61 gaatctgata ttttaaaatc agaagcatct ttgttgtatg aaaaaacaga acaatatcgc
      121 tacgcaattc tttctgaatc aatacaacgg aatgagcttc ctgaaataag gataacagat
      181 tttcctggcg gagaagataa tgcgggaggt gaacattttc agagggttag ctcattaata
      241 aaagagcaat tcatgacatg gcagaacaga aaaaatcaaa agcagctcac attaaataaa
      301 aaaatagttg aacgggatgc agcgcttgct cgtgttagcc tgtatgagca tcaggtatca
      361 caggaaggaa gaaaactcaa tgattttaag tatttgttga ataaaaaagc tgtttctcaa
      421 cattcagtta tggagcagga aaatagctat attcaggcaa aaaatgaaca tgcagtctgg
      481 cttgcacagg tttctcaact tgaaaaagaa atagaacttg tgcgggaaga actggcactg
      541 gagacgaata tcttcagaag tgaaattatc gagaagcaca gaaaatcaac agataacatt
      601 gtgttgctgg agcatgagct tgaaaaaaac aggcagagaa aagcatcatc ttttattaaa
      661 gctcctgtga gtggtactgt tcaggagtta aatatacata cagaaggtgg tgttgtcaca
      721 acagcagaaa cgctgatgat tattgtccct gataatgata ttctcgaagt aacagcatct
      781 gtacttaaca aggatatcgg ttttatacaa cctggacagg aggtcgttat taaagtagat
      841 gcatatccat atacacgtca tggttacctt acagggaaag taaaaaatat aactgcagat
      901 tctgtttctg ttccggatac agggcttgta tttaacgtga ttatatctgt tgatcggaat
      961 gatatacagg gagaaagaaa aaaaatccct gttacagctg gaatgactgt tatggcagaa
     1021 ataaaaaccg gggtgcgtag tgttatcagc tatcttctta gtccattaaa ggaaactatt
     1081 aatgaaagtt tacgtgaacg ttagtatata aataacatat tggcttttta tatgatatag
     1141 atttctgaaa taatcttgta aa
//
LOCUS       DAZFBR010000357         1129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFBR010000357 DAZFBR010000000
VERSION     DAZFBR010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.360"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            13..825
                     /locus_tag="ACHJZ2_005327"
     CDS             13..825
                     /locus_tag="ACHJZ2_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4571200.1"
                     /translation="MIYIINDEVRFNSSSNKLTSLLTSEEVILTYPAGKCLKLLLDNA
                     YSVVSHNQFFEKVWSDSSADVATNTLYQNISMIRRSFREISSATIFNDVIRTVPRKGF
                     KINEEISITVLDNNHNENESVESEENGTHTENINKNHIAKGIFTTKNTLLIVCFFAAW
                     GIVYISSYFFDMAFQSKSHTIKKIDAFSNYAQHPTQEGCSYFYDNKYFNSVDMLSPII
                     EKLQKMEINCKIHPYIYIATNDSMSGFYAFTCSQMLNSESPNNCNSLYYKAE"
     gene            830..>1129
                     /locus_tag="ACHJZ2_005328"
     CDS             830..>1129
                     /locus_tag="ACHJZ2_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HFO4571201.1"
                     /translation="MTQRYFLFAGIILCAFIAAILSHIAFHHANEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKIGFIRRYVSFTYKKFRDTV"
BASE COUNT          400 a          197 c          187 g          345 t
ORIGIN      
        1 gtataaaatg taatgatcta cattataaat gatgaagtaa gattcaactc atcatctaat
       61 aagttaacat cactccttac atcagaggaa gtgatactca cttatcctgc gggaaaatgc
      121 ttaaagttac ttttagataa cgcttatagt gtcgtgtcac acaatcaatt ctttgaaaaa
      181 gtctggagcg atagcagtgc cgatgtcgca accaacactt tatatcaaaa tatatcgatg
      241 ataagacgtt cttttcgtga aatcagctca gcgacgatat ttaatgatgt tatcagaacg
      301 gtgccccgta aaggcttcaa aatcaatgaa gaaatcagca taactgttct cgacaataat
      361 cacaatgaga atgaaagtgt tgagagtgaa gagaatggta cccacactga aaatataaat
      421 aaaaatcaca ttgcaaaagg tatatttacc acaaagaata cattacttat agtttgtttt
      481 tttgcggcat gggggatagt gtatatatct tcttattttt tcgatatggc ttttcaaagc
      541 aaatcccaca ctataaaaaa aatagatgct ttctcaaatt atgctcaaca ccctactcag
      601 gaaggatgca gttatttcta tgataataaa tactttaatt ccgtagacat gttatcaccg
      661 attattgaaa aattgcagaa gatggaaatt aactgcaaaa tacatcctta catttacatt
      721 gcaacaaatg atagcatgag cggattttat gctttcacct gtagtcagat gttaaactct
      781 gaatcaccaa ataattgcaa ctctctctat tataaagcgg agtaatcata tgactcaaag
      841 atattttctg tttgcgggaa taattttatg tgcttttatt gcagcaattc tgagccatat
      901 tgcgtttcat catgccaatg aaccagcaga gcaaaacata tcatgtaatg cccatgttat
      961 taatttcacc gatcatacaa aaatggatca atatttttcg ttaaatatga tttctgaaaa
     1021 taacacagga aacatgtatc tcacgggcgc atacacggta gatggaaaaa agataggctt
     1081 catccgccga tatgtgagtt ttacatacaa gaaattccgt gataccgtt
//
LOCUS       DAZFBR010000358         1129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFBR010000358 DAZFBR010000000
VERSION     DAZFBR010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.361"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            1..415
                     /gene="yqeJ"
                     /locus_tag="ACHJZ2_005329"
                     /pseudo
     CDS             1..415
                     /gene="yqeJ"
                     /locus_tag="ACHJZ2_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YqeJ"
     gene            complement(448..528)
                     /locus_tag="ACHJZ2_005330"
     CDS             complement(448..528)
                     /locus_tag="ACHJZ2_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571202.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(564..989)
                     /gene="yqeK"
                     /locus_tag="ACHJZ2_005331"
     CDS             complement(564..989)
                     /gene="yqeK"
                     /locus_tag="ACHJZ2_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO4571203.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          409 a          195 c          175 g          350 t
ORIGIN      
        1 tctattctta cactgcagaa aaaatgatat ataacgaaac atgcactgca aattgggtaa
       61 tttttaatga ccagggacga gcaaatctta ccatagactt tatgtataac aaaaaaataa
      121 aacaggaact gttgcgttga gtggaacctg gcaacaaggt aacagagaaa gtaaatcaat
      181 acggcgaaat attgaataca catgggttga aaactatgac acagcccatt taacatcaaa
      241 gaaagttaat aagtttgaga ttatggatca agtcgatgat gatagacttg cagaacttat
      301 tcctgatttt tatgtctttc cagaaaaaag tgtaagctat aatatactaa agcaaggtaa
      361 gcatgctttt attttgagca ttggtaacag agcaataatg cattgtgcaa ggtaaactaa
      421 aatagataac aaaaagatgg aacctcgtta atcataaaac aataaaatta aatattctcg
      481 cagtatatgg cagtctaaag catcaaagtt ttgatcaaca tctttcattt tagacatctc
      541 cttacaatgt aaaatatcat gtatcaacaa catctggtgc aaatccatga gtcgaactcg
      601 acattcatct tagctcgatt attattcctt cgtacagtcg atgtaaacaa tacagaaaga
      661 ggattattaa gaaccagttt actcagtgaa tcaatagagg aaaggactaa cgtttcttta
      721 aaagaattga tttcatcatc tgttaaacta aactcatcat tgacagatcg tgagatataa
      781 ctgtttttaa ctttactctt tacgttgact ttattgacag aattaacatt cacaaatctt
      841 gaatttaatg cccatgatgt tgtttgcgtg agaaccttct cagcattaag gaattttttt
      901 aacggaatcc ttggtttctt tttcgagtcc gaatttacaa tatcatgcat atagaccatt
      961 tcatgaatct gcgaaaactc aatgtccatt atatacctca catttttacc gtgactcgat
     1021 gttactgttc aataatcacc ttccatcaat actaaaatta atacccctaa tgtgccgata
     1081 acaaatatag tcattctacg taacgtctcc ataaggtgat aaccttgat
//
LOCUS       DAZFBR010000359         1118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFBR010000359 DAZFBR010000000
VERSION     DAZFBR010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.362"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>1118
                     /locus_tag="ACHJZ2_005332"
     CDS             <1..>1118
                     /locus_tag="ACHJZ2_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HFO4571204.1"
                     /translation="DAASRTLTLDREVTLPETGTSTVNLINGSGKPVSVDITAHPAPD
                     RIQVSTLPDGVATYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVD
                     NGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSG
                     SGEGSRLVTTAITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAVPA
                     TIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWSVSG
                     PHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAAGYLELFREKIGKTHLAEALWAE
                     IDNSQLKDEMAEMQTTITETRNEITQTVSKTLEDQSAT"
BASE COUNT          259 a          311 c          351 g          197 t
ORIGIN      
        1 gatgccgcca gccgcaccct gacgctggac cgtgaggtga cactgccgga gacaggtaca
       61 tcgacggtga acctgattaa cggcagcggt aagccggtga gtgtggacat caccgcacac
      121 cccgcgccgg accggataca ggtcagtacc ctgccggatg gcgtggcgac atacggtgtg
      181 tggggactct ccctgccgtc actgcgtcgt cgcctgttcc gctgtgtttc catccgggaa
      241 aacacggacg gcacctttgc catcacggcg gtgcagcacg taccggaaaa ggaagccatc
      301 gtggataacg gggccagctt tgagccgcag tcaggcaccc tgaacagcgt catccctccg
      361 gcagtgcagc acctgacggt ggaggtgagc gcagctgacg gccagtatct ggcacaggcg
      421 aaatgggaca cgccgcgggt ggtgaagggg gtgcgcttca gtctgcgact gaccagcgga
      481 agcggagaag gcagccgtct ggtgaccacc gccatcaccg cggatacaga gcaccgtttc
      541 agtggtctgc cgcccgggga atacaccctg acagtcaggg caattaacag ttatggccag
      601 cagggcgaac cggccaccac cacgttcagg attaatgcac ctgcggtacc cgccacgatt
      661 gagctgacac cgggctattt tcagataacg gcggtcccgc gtcttgcggt gtatgacccg
      721 acggtacagt ttgagttctg gttttcggag acaaaaatcg cagacacatc tcaggtggaa
      781 acctctgccc gttatctggg gaccggcagt cagtggagtg tatccggccc gcacattaag
      841 cccgggaagg atttctggtt ttatgtgcgc agcgtcaacc tggtgggtaa atctgctttt
      901 gtggaagcca gtggacgggc gagcaatgat gctgcgggct atctggaact tttccgggaa
      961 aagataggaa aaacgcatct ggcagaggcg ctgtgggcag agattgacaa cagtcagctg
     1021 aaggacgaga tggcggaaat gcagaccacc atcacagaaa cccgcaatga aatcacacag
     1081 acggtcagta aaacgctgga agaccagagc gccaccat
//
LOCUS       DAZFBR010000360         1112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFBR010000360 DAZFBR010000000
VERSION     DAZFBR010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.363"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(1..666)
                     /gene="sctT"
                     /locus_tag="ACHJZ2_005333"
                     /pseudo
     CDS             complement(1..666)
                     /gene="sctT"
                     /locus_tag="ACHJZ2_005333"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
     gene            complement(668..928)
                     /locus_tag="ACHJZ2_005334"
     CDS             complement(668..928)
                     /locus_tag="ACHJZ2_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4571205.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(939..>1112)
                     /locus_tag="ACHJZ2_005335"
     CDS             complement(939..>1112)
                     /locus_tag="ACHJZ2_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4571206.1"
                     /translation="VVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSK
                     GLILQYFDLSINP"
BASE COUNT          387 a          242 c          193 g          290 t
ORIGIN      
        1 cagcgcactt ttgacagtca gtgaaatggc aaaagcattt agttgtgaag catagcgtgc
       61 cagcaatccc aggacagcct caccacccag cataacggca attacggggc ttgcgtaaac
      121 gatagtatga gtcattatat tattaatata tgaaaataaa ggctctaatt taggaaaatt
      181 aaaacttcct gaaggccata aattataact ttgctaaaga gtttccagaa taaagttcaa
      241 tccaccgtta gtaagataaa ccgctgcaga gaaaaggtta aataatcttg ctaattccga
      301 ggtatcgaca cctgtagccg gatccaaagt actacttaac gttgcaccgc gttggttatc
      361 gataaagcta cctatagcaa ggaaaatcca aaatggagat gctaataagc aagcaatgaa
      421 cagccctatg ataatttctc ttgcagctaa caaagcgata tcgatttcat ttaaggtcga
      481 taattcatag tggtaatgct gaactaatgc acatgatacc actattatta ttggatgacg
      541 gactactgtt ggtatattcc cactggcgaa aaatggtaac aaatagaaag ttggtgccaa
      601 tcggcaaaaa ccgagcgctg tagcagccag tagactatgc aactgataga gtatcacctc
      661 ccccatgcta tcctctcgca aatgctaaat tcagcatttc aataccaaaa gaatataatt
      721 tttcaccata ccatccgctc attaaaaaaa agcaaatact tacgcacaat aatttcacgc
      781 caaatggcaa cgtctgttct tgcaactgcg ttaccgtttg aaaaagccct accaataaac
      841 caacaaatgt cgctactgca ataggtcctg cagacataac taaaatcaga tacagcgccc
      901 tattccctgc aaaaacaatg tcatccatat ttttttcctc agggattaat ggataggtca
      961 aaatattgta atatgagtcc ttttgataac attgtccaac cgtccatcgc gacaaacaga
     1021 attaatttta tcggtgtaga tatggttacc ggactcatca tcatcatacc taacgttaac
     1081 agaacgctgg atataaccag atccactaca ac
//
LOCUS       DAZFBR010000361         1109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFBR010000361 DAZFBR010000000
VERSION     DAZFBR010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.364"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..1045)
                     /locus_tag="ACHJZ2_005336"
     CDS             complement(<1..1045)
                     /locus_tag="ACHJZ2_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO4571207.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKLKFDSLNPVNGLKRIFGLKTVKEFVKAILYIIFFALEIKVFWSNHKSLLF
                     KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKRE
                     YKEQEGNPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPL
                     ISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLW
                     LEDVENAGQPVPDE"
BASE COUNT          361 a          207 c          167 g          374 t
ORIGIN      
        1 gttcatctgg aacaggttgt ccagcattct ccacatcttc aagccacagc agaagacgta
       61 atatttcatc tatattttcg aagctaacat aatcgtagcg acgatgggta gcataaattt
      121 ttcgtgctaa ttttttatct gtaataattg gtatcccgat ttcctttgca tatttcctta
      181 cagctaatgc tacctcatta gtttctctta cagaaatcaa tggaataggt gacagatgtg
      241 gcttaaagta tatccctatt gcaatgtgag tagggttggc aatcatcaaa cggctattac
      301 tgacatcaga tttcaattgc tcagaaagaa tttcctgatg gcgctctctg cgtttagact
      361 taatttcagg atttccttct tgttccttgt attctctttt aacttcttgt ttatccattt
      421 tcatatcttt cataaataaa aaatattcag cgataaaatc aaaaattaag acaattatca
      481 tactgccgag acaatacagt atgagaagga atagcatctc tccccaatct gataataaag
      541 atatgatatc tccatcaaga gttttaaaaa gcagtgattt atgattactc caaaatactt
      601 tgatctccaa tgcaaaaaaa ataatataaa gaattgcttt aacaaattct tttacggttt
      661 ttaagccaaa tatacgcttt aaaccattta ctggattcaa tgaatcaaac tttaatttta
      721 cagcttcagt tgctaattgc attttacttt gaaaccagga gacaagtatt gttgtgaaca
      781 tgcaaaccag caaaaaagga atgatcgttt tcaaccaacc aatccccata gctttaaaat
      841 agtcccaaat atccggttta gcgttatgat caaggatata ttcaagaatc gacataatgt
      901 gatgcacatc aaaaacatat ccgagatata aagtacccac aagcattatt acagaaacgg
      961 ttaagtctct actttttaga atttgccctt ttttagaagc atcctgcagt ttcttttgtg
     1021 ttggcttttc agttttattt gccatgaaac actctatttc tcaatgtata gttgcaattg
     1081 ttctgggaaa aaggataacg gcatcactc
//
LOCUS       DAZFBR010000362         1105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFBR010000362 DAZFBR010000000
VERSION     DAZFBR010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.365"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            126..>1105
                     /locus_tag="ACHJZ2_005337"
     rRNA            126..>1105
                     /locus_tag="ACHJZ2_005337"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          295 a          240 c          345 g          225 t
ORIGIN      
        1 agacaatctg tgtgggcact cgaagatacg gattcttaac gtcgcaagac gaaaaatgaa
       61 taccaagtct caagagtgaa cacgtaattc attacgaagt ttaattcttt gagcatcaaa
      121 cttttaaatt gaagagtttg atcatggctc agattgaacg ctggcggcag gcctaacaca
      181 tgcaagtcga acggtaacag gaagaagctt gcttctttgc tgacgagtgg cggacgggtg
      241 agtaatgtct gggaaactgc ctgatggagg gggataacta ctggaaacgg tagctaatac
      301 cgcataacgt cgcaagacca aagaggggga ccttcgggcc tcttgccatc ggatgtgccc
      361 agatgggatt agctagtagg tggggtaacg gctcacctag gcgacgatcc ctagctggtc
      421 tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac gggaggcagc
      481 agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt gtatgaagaa
      541 ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggg agtaaagtta atacctttgc
      601 tcattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc cgcggtaata
      661 cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg cggtttgtta
      721 agtcagatgt gaaatccccg ggctcaacct gggaactgca tctgatactg gcaagcttga
      781 gtctcgtaga ggggggtaga attccaggtg tagcggtgaa atgcgtagag atctggagga
      841 ataccggtgg cgaaggcggc cccctggacg aagactgacg ctcaggtgcg aaagcgtggg
      901 gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcga cttggaggtt
      961 gtgcccttga ggcgtggctt ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg
     1021 ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca caagcggtgg agcatgtggt
     1081 ttaattcgat gcaacgcgaa gaacc
//
LOCUS       DAZFBR010000363         1100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFBR010000363 DAZFBR010000000
VERSION     DAZFBR010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.366"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(1..510)
                     /locus_tag="ACHJZ2_005338"
                     /pseudo
     CDS             complement(1..510)
                     /locus_tag="ACHJZ2_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
     gene            complement(623..892)
                     /locus_tag="ACHJZ2_005339"
     CDS             complement(623..892)
                     /locus_tag="ACHJZ2_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4571208.1"
                     /translation="MNILKKLMQRLCGHGKHDDREDGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(894..>1100)
                     /locus_tag="ACHJZ2_005340"
     CDS             complement(894..>1100)
                     /locus_tag="ACHJZ2_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4571209.1"
                     /translation="ASIIETNSHGWFPGTDGALITGLTFLAPKDTTRVQGFFQHLQVR
                     FGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          290 a          300 c          210 g          300 t
ORIGIN      
        1 tcattgatgc cgttattggt tctcgggtca gtggatgata tgagccttca ctagctaaac
       61 gagaaaatgc atcaaaatca aataagcagc atactgccga actatctgaa tttttgacaa
      121 acactccttc ttcgggtctc tctagtgtaa ttggacactg aatagactct ggcgggcact
      181 ggaaatttcc tgaactaaca ggaaacttac attgagatat tttgtcctga atactctctt
      241 gcgattgtgc ttcacctgag tccgaaagca tactgagcat cctatcgcga agagcttcag
      301 ggccgttatt aaacccataa aggaataact cagtaagcaa tcccccacta ctgccgctgg
      361 atagaaaacg cccatcggtt tcattaaata caatgctgac agtctcatta cctaatgtaa
      421 actcccctcg accgctagat gctacttgag ctcgaaggac attcaatcca ttaaaaatga
      481 gcttgatcta atctccgaat ttaacggcat tataaaactc cctattttaa tttaaactcc
      541 agacttaaat agctttaaca aacatctgcc ttacatgaca acaaaaaccg gagccggact
      601 ccggttttgt gaagctgtcg ggttacttca tcccgccaat attttcccac gtcccgtcag
      661 cacgcaggat ttgcagcggt cttaccacgc actgtatctg ctttttatcc gcatccagta
      721 tcaccacctg cgtgattacc ctgtcctgct ccggaataat accattctca tcggactcca
      781 ggatgtctgc cggccccaga cgcagttgtg ctgtaagtaa ctccccgtct tcacggtcat
      841 catgctttcc gtgcccgcac agacgctgca taagtttttt tagtatgttc atgtcattct
      901 cctgttctgc ctgtatcact gcccacttca tcaagcccct taacatcctg ccacggcccg
      961 tcaccaaacc tgacctgcaa atgctgaaaa aaaccctgaa cccgtgtggt atctttgggg
     1021 gcaagaaagg tcagtccggt gatgagcgca ccatctgtac ccgggaacca gccatggctg
     1081 tttgtctcaa taatgctcgc
//
LOCUS       DAZFBR010000364         1099 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFBR010000364 DAZFBR010000000
VERSION     DAZFBR010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.367"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..989)
                     /locus_tag="ACHJZ2_005341"
     CDS             complement(<1..989)
                     /locus_tag="ACHJZ2_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4571210.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATRAPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPEPRIKQLARRSTVTPGGAACRYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMT"
BASE COUNT          258 a          339 c          247 g          255 t
ORIGIN      
        1 gtcatgaacg gtacgaaatg gatattcttt tccgttttgt ttttgtagct gccgtacacc
       61 gtctggtacg tggattcgtt cttctgcttc cagaaatacg tcgtatctcc acatatccag
      121 ggaacaccgc cagcagagcc accgacgcac tgacctgcca tatccgccag gtctgcacgg
      181 aatttatcaa ccagcgcacc aaactgtgct gcgtgatttg ccggcgtacc gccaaaatca
      241 aattccccct gcatccacac cacggcaaac agcacatttt tcgggttctt cttcagtgct
      301 gcttttgttc gaccgataag gtccttatac agcggcttgt ccacacccca gcgggttgaa
      361 ttctccgagg caccactcgc gtcactgtat gtgccatcag ctccggtggt gaacgctgaa
      421 ccaccacgac agcacggaac cagcagaatg cccgcattcg ccggtataaa cggcagcaat
      481 tttttggcga tatgcagccc ctgccccacg gttccgtact gcccctttga caggtccgct
      541 ttcggatggt taagacggct catgtcctgc acatcatgca gacaatggtc cgccggaatg
      601 atgtcgttat atctgcatgc tgcaccaccc ggtgtcaccg tactgcgacg cgccagttgc
      661 ttaatacgcg gctccggacg gtcatatgtc tccggcagcg gaagaccttc accatacgac
      721 atgccgtttg actgccctgc cagaaccaca acaaagtaat actccggggc tctggtggca
      781 ctgacagcca ccgccttacc ctcattaccg gtcaccgcca ctggtgtggt gacatcacct
      841 tccgccgcaa tggcctgcat cagggtgtaa ggcgtgatgg ccaccggact gccaaatggc
      901 tgccagccct ccttcagttt ttgtgtcagt cgttcagcaa ggtctgacgg cgatgccgcc
      961 ctgaccacat cgtagtgttt aaatgtcata acctctcctg ccgccagcaa agtaattatc
     1021 cggaacacac aatcatgtaa cacataaaaa acacgctata aaaaaaccat gtctcttata
     1081 taacaaaaaa acgccgaca
//
LOCUS       DAZFBR010000365         1096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFBR010000365 DAZFBR010000000
VERSION     DAZFBR010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.368"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            32..>1096
                     /locus_tag="ACHJZ2_005342"
     CDS             32..>1096
                     /locus_tag="ACHJZ2_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFO4571211.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYANEILEIKRIMGEYNLLPDKNSRNGLKLLQKQADL
                     LKIIMEDTSVTENTFKNIEMAIADIKREYYSHTVDIEKNIHAIWVAGSPPESISDYIK
                     TFLKTYKEFTYYLWVDEKAFG"
BASE COUNT          429 a          188 c          199 g          280 t
ORIGIN      
        1 cataaataat tttttaagcg gaaatgtaat tatgagactg ccagagaaag ttctttttcc
       61 tcctgtcact agtggcctgt cagggcagga aaaacaaaaa aaaccgaaga gcattaccgg
      121 atttcaggaa aattatcaac gcaatatcag gccaatcaaa acagcatcag aagcccgact
      181 acgcttcttt gataaaatgg tttcgaaaga aaactctctg gaagatgttg tttctttagg
      241 tgaaatgatt cagaaggaaa tttatgggca tgaacaaaga acattttcac cagttcatca
      301 tacaggtaac tggaaatcat cattgttaca caacgcgctc cttggtctgg caaatgttta
      361 taatggctta cgggaaacag aataccctaa cactttcaac agagatggta taaaaagtac
      421 taactctttt agagataact tattgacaaa aacaagaact cccagagata attttgagga
      481 aggaataaaa catcctgaac atgcaacaat accatatgac aacgacaatg aaagtaataa
      541 attgctaaaa gcaggaaaga tagctggtaa caataacgag ctgttgatgg aaataaaaaa
      601 ggaatcccaa agcgaccatc aaatccccct gtcagataag ttcctgaaaa ggaaaaaacg
      661 atctcctgta gctgaagata aagttcaaaa ctcgttaaca ccagaaaatt ttgttcagaa
      721 aatttcactt agtgatgagc ttaaaacaaa atatgcaaat gaaattctag agataaaaag
      781 aataatggga gaatacaatc ttttaccgga taaaaacagt cgtaatggtc taaaacttct
      841 acagaagcaa gctgatttac taaaaataat catggaggat acctctgtta cagaaaatac
      901 ctttaaaaac atagagatgg ctatagcaga tataaaaagg gagtactatt cgcatacagt
      961 tgatattgag aaaaatattc atgccatatg ggttgcgggt tccccacctg aaagcatctc
     1021 ggactatatt aagacttttc tcaaaaccta caaagagttt acttactatc tttgggttga
     1081 tgaaaaagca tttgga
//
LOCUS       DAZFBR010000366         1056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFBR010000366 DAZFBR010000000
VERSION     DAZFBR010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.369"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(418..653)
                     /locus_tag="ACHJZ2_005343"
                     /pseudo
     CDS             complement(418..653)
                     /locus_tag="ACHJZ2_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(812..1039)
                     /locus_tag="ACHJZ2_005344"
     CDS             complement(812..1039)
                     /locus_tag="ACHJZ2_005344"
                     /inference="COORDINATES: protein motif:HMM:NF018156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4571212.1"
                     /translation="MTLAIPQRGVFLRNAEGSRVCSLYDEMALSRIINDGMHHPLSRE
                     PITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
BASE COUNT          318 a          247 c          190 g          301 t
ORIGIN      
        1 tgacagggct atttaaacaa tcactaaaaa tcgcccaaga gcattctgtt tctataatgt
       61 attccaagag attaaaaaat aatatcatac gttattcggc agagttcgta tgtactacgg
      121 cgtcccaacc atgtcccaaa acaaaaattt ctgccctata cacgttccaa aaaattcaac
      181 agcaaagaga gtctgctgac gagttgttga ttcactgatt aatacgtaaa attgataact
      241 gctggaaatc atacaatact cgtactttcg aaagttcgct aaccaggtgc tacacgcgag
      301 gtgttgcagt tttctttacg atttatcatt caatcctaat ccatgcatga catgtggtgc
      361 ctgtggggtg atcctaccta cgtaatgtgg acacagccct aatcaaggtt ctgtttttca
      421 aaacagttcc gtgctgagac agccacaagt actgtgtcgg caccaccgat tgtaatcaca
      481 ctagatctaa ttaaacacag ccgtctgcat tatttcccga ctcataaaat attagccgta
      541 aatgcattcg acttttagtg agtggaagaa actttccgcc ctagcattat cgtaacagcg
      601 cccctttgca ctcatcctgt catgcaggta tgccgcttca gaagcgcctg ataatgcgtt
      661 gaaatcagga cggtggctac ttgctattca gttcacgata catcccttaa ggaccagcga
      721 atagcaatga gtcactttat tatcaagttt gatgaccgct ttgatgctaa cttttaataa
      781 gagtctcttc acacaaaatc gtgtaccgag tctacactga ataacaatca ctcctcaccg
      841 taaagtgacc gatactgcaa tcaaactcac actgctctct ggccacaagc attgataatg
      901 ttattggctc tctgcttagt gggtgatgca tcccatcatt aattatacga gaaagagcca
      961 tttcatcata taaactacat actctggaac cttcagcatt tcttaaaaaa acacctcgtt
     1021 gaggaattgc cagcgtaatc gggcactgta atgctt
//
LOCUS       DAZFBR010000367         1055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFBR010000367 DAZFBR010000000
VERSION     DAZFBR010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.370"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..888
                     /locus_tag="ACHJZ2_005345"
     CDS             <1..888
                     /locus_tag="ACHJZ2_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4571213.1"
                     /translation="MPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSAHA
                     QHDDWLKKEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGK
                     KVRTTVSRKAVSAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGCIV
                     GWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKEAGLLASTG
                     STGDSYDNAMAESINGLYKAEVIHRKSWKNRTEVELATLTWVDRYNNRRLLERLGHIP
                     PAEAEKAYYASIGNNDLAA"
     misc_feature    <1..72
                     /locus_tag="ACHJZ2_005345"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            932..1055
                     /locus_tag="ACHJZ2_005346"
                     /pseudo
     CDS             932..1055
                     /locus_tag="ACHJZ2_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vimentin yjdA"
BASE COUNT          268 a          249 c          312 g          226 t
ORIGIN      
        1 atgccactgc tggataagct gcgtgagcag tacggggtcg gaccggtatg cagcgaactg
       61 catattgccc cgtcaacgta ttaccattgt cagcaacagc gacatcatcc ggataaacgc
      121 agtgcccatg cgcagcacga tgactggctg aagaaagaga tacagcgcgt atacgatgaa
      181 aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacgcga aggtatcagg
      241 gtggccagat gcactgtggc acgtctcatg gcggttatgg gacttgccgg tgttctccgg
      301 ggtaaaaagg tccgcactac cgtcagccgg aaagccgttt ccgcaggcga ccgcgtaaac
      361 cgtcagttcg tggcagaacg tcctgaccag ctgtgggtgg ctgattttac ttacgtcagc
      421 acatggcagg gcttcgtcta tgtggcgttc atcattgatg tgtttgccgg atgtatcgtg
      481 gggtggcgag tctcatcgtc tatggaaacg acattcgtgc tggatgcact ggagcaggcg
      541 ttgtgggccc gtcggccgtc cggcacaatc catcacagtg ataaaggttc tcagtatgta
      601 tcgctggcct acacgcagcg gcttaaggaa gccggattac tggcatcaac agggagtact
      661 ggcgactcgt atgacaacgc gatggctgag agcatcaatg gtctttacaa agcggaggta
      721 atacaccgta agagctggaa aaaccggaca gaagtggaac tggccacact aacgtgggtg
      781 gacaggtata acaatcgacg attgctggaa aggctgggcc atattcctcc ggcagaagca
      841 gaaaaagctt attatgcttc catcggaaat aatgatctgg cagcctgagt tcacagataa
      901 aatactctcc aggaaacccg gggcggttca gtctcagtcc ggattatggg ggaaaatttc
      961 tcaatggtcc ggtatcaaca accagggccg ggaggattac aggaaacgag attttgcccc
     1021 ggacagcccc gaaataaaat tcagtgatcg caggg
//
LOCUS       DAZFBR010000368         1049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFBR010000368 DAZFBR010000000
VERSION     DAZFBR010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.371"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            1..186
                     /locus_tag="ACHJZ2_005347"
     CDS             1..186
                     /locus_tag="ACHJZ2_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4571214.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQTRIPTASQNRSPAPVF
                     VVMSGEYLLDKKEEIIQ"
     gene            199..288
                     /locus_tag="ACHJZ2_005348"
     CDS             199..288
                     /locus_tag="ACHJZ2_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571215.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            308..>1049
                     /gene="nleL"
                     /locus_tag="ACHJZ2_005349"
     CDS             308..>1049
                     /gene="nleL"
                     /locus_tag="ACHJZ2_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO4571216.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCS"
BASE COUNT          304 a          188 c          238 g          319 t
ORIGIN      
        1 gtgaacacgg cagcggaagc catccgtgcc ctgtcgctgc agatgccggg attccgccgt
       61 cagatgaacg aaggctggta ccagacacgt attccgacag cttcacaaaa ccggagtccg
      121 gctccggttt ttgttgtcat gtccggggaa tatttgttag ataaaaaaga ggagataatt
      181 caatagggag ttaaattaat gccgataaat ctgacatctt atttgtgggt acagggacag
      241 aaagttgtcc cggcagttgt gttttctaat tttactttag tggactaagt aaaaaggagt
      301 gagataaatg ctgcccacta caaatatctc tgtaaattct ggagtaatat cttttgaaag
      361 tcctgtagat tcaccatcta acgaggatgt tgaagttgcc ctcgaaaagt ggtgtgctga
      421 gggagaattt agcgaaaatc gtcatgaggt tgcatcaaaa atacttgatg ttataagtac
      481 taatggagag actttatcaa tcagtgagcc aataacaaca ttaccagact tgcttccagg
      541 ttctctgaaa gaactggttt tgaatggatg tacagagctt aaatcaataa actgcttacc
      601 ccccaactta tcttcattaa gtatggttgg atgctcatca ttagaggtta taaattgcag
      661 catacctgaa aatgtcatta atttatcttt atgccattgt agttctttga aacatataga
      721 aggttccttt cctgaggcac tcagaaattc cgtatattta aatggctgta attcattaaa
      781 tgaatcgcaa tgtcaattcc ttgcatatga tgtcagtcaa ggccgtgcct gcctgagcaa
      841 agctgagctt actgctgact taatttggtt gtcagctaac cgaacgggtg aagagtctgc
      901 tgaagaattg aattactctg gatgtgactt gtcaggtcta agtcttgtag ggctgaattt
      961 atcatcagta aatttttctg gagcagtgct tgatgataca gatctcagga tgagtgattt
     1021 gtctcaggct gtattggaaa actgttctt
//
LOCUS       DAZFBR010000369         1029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFBR010000369 DAZFBR010000000
VERSION     DAZFBR010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.373"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>1029
                     /locus_tag="ACHJZ2_005350"
     CDS             <1..>1029
                     /locus_tag="ACHJZ2_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO4571217.1"
                     /translation="KDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQ
                     GGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLI
                     RSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVTVHYGY
                     DEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWL
                     AGMKLGDTPLVDFTRDRLHRKTLRRFGRYELTTAYTPAGQLQSQHLNSLQYDRDYTWN
                     DNGELIRISSPRQTRSYSYSDSGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPD
                     STLSMWPD"
BASE COUNT          248 a          333 c          304 g          144 t
ORIGIN      
        1 gaaagacacg cagggccatg aaacgcggta tgaatacaac gccgccggtg acctgaccac
       61 cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg gaaaagccat
      121 ctgtaccacg cagggcggtc tgacgcgcag tatggaatac gatgctgccg gacgggtcat
      181 ccgcctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac tcgaccggct
      241 gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc tgaccggcaa
      301 acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg aagcagaccg
      361 cctcacgcac cgcaccgtga agggtgaaac cgcagagcgc tggcagtatg acgaacgcgg
      421 ctggctgaca gacatcagcc atatcagcga agggcaccgg gtgacggtgc attacgggta
      481 tgatgagaaa ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc
      541 actgctctgg cagcatgaga ccagacacgc ttacaacgcg caggggctgg cgaaccgctg
      601 tataccggac agcctgcccg ccgtggaatg gctgacctat ggcagcggct ggctggcagg
      661 catgaagctc ggcgacacac cgctggtgga tttcacccgc gaccgcctgc accggaaaac
      721 gctgcgcaga ttcggccgtt atgaactcac caccgcttat acccctgccg ggcagttaca
      781 gagccagcac ctgaacagcc tgcagtatga ccgcgattac acctggaacg acaacggcga
      841 actcatccgc atcagcagcc cgcgccagac ccggagttac agctacagcg actccggcag
      901 gctgaccggc gttcacacca ccgcagcgaa tctggatatc cgcatcccgt atgccacgga
      961 cccggcaggt aaccgcctgc ccgacccgga gctgcacccg gacagcaccc tcagcatgtg
     1021 gccggataa
//
LOCUS       DAZFBR010000370         1025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFBR010000370 DAZFBR010000000
VERSION     DAZFBR010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.374"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..711)
                     /locus_tag="ACHJZ2_005351"
     CDS             complement(<1..711)
                     /locus_tag="ACHJZ2_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4571218.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIC"
     gene            complement(717..>1025)
                     /locus_tag="ACHJZ2_005352"
     CDS             complement(717..>1025)
                     /locus_tag="ACHJZ2_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4571219.1"
                     /translation="VISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCT
                     WTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ
                     "
BASE COUNT          227 a          326 c          270 g          202 t
ORIGIN      
        1 gcaaatcccc gtaaaggcag atgcgcgcca tgcccggtga cgccagaggg agtgtgtgcg
       61 tcgctgccat ttgtcggtat acctctctcg tttgctcagt tgttcaggaa tatggtgcag
      121 cagctcgcca tcaccacagt aaatggcggc atgattcggc accgatgaac caaaacagca
      181 cagcagcaca tcgcccggtt gtgctgatga caacggcacc tgatacagcc ctgtggcctc
      241 cagattatcc agatagagat tctgaccgtg acgccaccag tcatcctcgc gatgaaaatc
      301 cggcatctca atccccgcca gatgataagc atcccggaac agcgtgtaac agtccgtcac
      361 cccgtgctca aagcgccgcc cggtgagatg cggcacacag cggaacttat gaatcgtccc
      421 ccggcagacc agccaccacg gcaaatcact ctgcacctgc agccgccggt cggcctcact
      481 cagccagggc agaccaccgg ggtggctgtg gaccagcgcc acaatctcac cctgcatttc
      541 tgcctgcagc cagtcttccg gcgacatacg gaaatacgcc tccggctcac cggagatatt
      601 cacgcagggg aaatatcttt ccccctccgg cgtgcttacc acgaagccgc acgactccgc
      661 tggcgcacat cgccgggcgt gcgccagaat cgctgattct gtctgtgtca tgggatttac
      721 tgcgaaagtt tgttaatgga aaggaagccg ccaaagttgc cgacgttatt gcggaactta
      781 caaccgctca ggcatttgct gcatttatcc ttcgtgatat cggacgttgg ctggtcatat
      841 tcatccgcga ccgccggacc gtgataaccg cactcatcgc cgcgataggt ccaggtgcag
      901 gtgttggcca gcatgatacg tcccggaaaa acagcgccgt ccgtttccgt cggcgtggac
      961 agtacaaaag aggcactcac cgcgctcagt tcgctgcact gctcaatgcg ccagcggctg
     1021 atcac
//
LOCUS       DAZFBR010000371         1025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFBR010000371 DAZFBR010000000
VERSION     DAZFBR010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.375"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..96)
                     /locus_tag="ACHJZ2_005353"
     CDS             complement(<1..96)
                     /locus_tag="ACHJZ2_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571220.1"
                     /translation="MVCLYNFGVRAAMDNSKHSDEAEKLLAELSAR"
     gene            complement(129..>1025)
                     /locus_tag="ACHJZ2_005354"
     CDS             complement(129..>1025)
                     /locus_tag="ACHJZ2_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4571221.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGL
                     YYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSPSDPI
                     YKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEGMPVV
                     LFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGKDNGV
                     FELVPDEKQPGDFHKFTPTGSK"
BASE COUNT          238 a          291 c          202 g          294 t
ORIGIN      
        1 tctagcagaa agctccgcca ataatttttc cgcctcatca gaatgttttg agttatccat
       61 agcagccctc actccaaagt tataaagaca taccatgata atcaacaaaa aagtaaatac
      121 tttcattact atttgcttcc tgtaggtgta aacttatgaa agtcccctgg ttgtttctcg
      181 tctggcacca attcaaacac cccgttgtcc ttaccgccta ttgttatcct agctgtttgc
      241 cccataatag catagtttcc atccggccaa atccatatta tttcatcagg agcaatcact
      301 gtagcatcaa gttcgttagc tagtttctgt gcaaatgaat ttttgccttt tcctgtttcg
      361 caggaaaaca agactacagg cataccttca tgatatttag gattcgctct tatgtcgcta
      421 gctaattgtt ttattgtcag atcacttcct tttttgattc tgtcatcact catagatgta
      481 ggcgaaccat gacctccaac tgtaaaaccc gttggaaatt cccttacatt aagagaacct
      541 ttatatatag gatctgacgg agaataataa ttcaaatcaa gtgctaaccc caacggatct
      601 atatgttcaa taggattgag tgggtactga tacaggttcc atcccccctc cagccctatc
      661 gggtcctggg tgatatatct cccctgcaac ggatcatagt accggttgcg gttgtagtac
      721 agccccgact cctcatcata ctgctgcccc ggcagacgga gtgactgttg aaggtgctgc
      781 gcgctggttt cgcccagcag gtttccccac tcgtcatact ccccctgcca cgccgtttcc
      841 ccttccgggc tgatgagcgc cagcggcagt ccccggtggt cgcagtggta aagatgaagt
      901 ttcctctccg ggatgtattc cgcctccagc tgcgcggcca tctgctccgc cgtcagcccg
      961 cactgcgcaa gccactgctg gctttcttca ctcacgctgc cctgccgcag ctcccgctcc
     1021 agcct
//
LOCUS       DAZFBR010000372         1004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFBR010000372 DAZFBR010000000
VERSION     DAZFBR010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.376"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>1004
                     /locus_tag="ACHJZ2_005355"
     CDS             <1..>1004
                     /locus_tag="ACHJZ2_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO4571222.1"
                     /translation="SLPAAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVG
                     HRYAGRPEMRYRYDDAGRVVEQLNPAGLSYHYQYEQDRITVTDSLNRREVLHTEGGAG
                     LKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRET
                     KFYYNDGNQLTAVVSPDGLESRRAYDEPGRLVSETSRSGETVRYRYDDAYSELPATTT
                     DATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGR
                     LISVKDAQGHETRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYD
                     L"
BASE COUNT          229 a          281 c          353 g          141 t
ORIGIN      
        1 agcctgcccg ctgcgccact ggtgcggtac acgtatacgg aagccggtga actgctggcg
       61 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
      121 cggatggtgg ggcaccgtta tgcgggaagg ccggagatgc gctaccgcta cgacgatgcg
      181 gggcgggtgg tggagcaact gaacccggca ggcctgagtt accactacca gtatgagcag
      241 gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
      301 gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg
      361 tatgacgcgg caggaaggct aacggcgcag acggacgcgg cgggacggcg gacagagtat
      421 ggtctgaatg tggtatccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
      481 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
      541 agccgccggg catatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
      601 acagtacgct accgctacga tgatgcgtac agtgagttac cggcgacgac aacagatgcg
      661 acgggcagca cccggcagat gacctggagc cgctacggtc agttgctggc gttcaccgac
      721 tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggtccac
      781 cgcgaggaag gcatcagcct ttaccgccgc tatgacaacc gtggccggtt aatctcggtg
      841 aaagacgcac agggccatga aacgcggtat gagtacaacg ccgcaggcga cctgactgcc
      901 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
      961 agcaccacgc agggcgggct gacgcgcagt atggaatacg acct
//
LOCUS       DAZFBR010000373         1004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFBR010000373 DAZFBR010000000
VERSION     DAZFBR010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.377"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>1004
                     /locus_tag="ACHJZ2_005356"
     CDS             <1..>1004
                     /locus_tag="ACHJZ2_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO4571223.1"
                     /translation="SLPGAPLARYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVA
                     HRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRITVTDSLNRREVLHTEGGAG
                     LKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRET
                     KFYYNDGNQLTAVVSPDGLESRRAYDEPGRLVSETSRCGDVIRYAYDNPHSELPATTT
                     DATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISRYRRYDNRGR
                     LTSVKDAQGHETRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYD
                     L"
BASE COUNT          225 a          279 c          352 g          148 t
ORIGIN      
        1 agcctgcccg gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg
       61 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcatccgggc
      121 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacc
      181 gggcgggtgg tggagcagct gaacccggca ggcctgagtt accgctacca gtatgagcag
      241 gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
      301 gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg
      361 tatgacgcgg caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac
      421 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
      481 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
      541 agccgccggg catatgatga accgggcagg ctggtatcgg agacatcgcg ctgtggggac
      601 gtcatccggt atgcttatga taatccgcac agtgaattac cggccacgac aacagatgcg
      661 acgggcagca cccggcagat gacctggagc cgctacgggc agttgctggc gttcaccgac
      721 tgctcgggct accagacccg ttatgaatac gaccgctttg gtcagatgac ggcggtccac
      781 cgtgaggaag gtatcagccg ttaccgccgc tatgacaacc gtggccggtt aacctcggtg
      841 aaagacgcac agggccatga aacgcggtat gagtacaacg ccgcaggcga cctgactgcc
      901 gttatcactc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaagcggtc
      961 agcaccacgc agggcgggct gacgcgcagt atggagtatg acct
//
LOCUS       DAZFBR010000374         1003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFBR010000374 DAZFBR010000000
VERSION     DAZFBR010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.378"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            132..932
                     /locus_tag="ACHJZ2_005357"
     CDS             132..932
                     /locus_tag="ACHJZ2_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571224.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          319 a          181 c          222 g          281 t
ORIGIN      
        1 tttatattga ttaaatgaat gtatatttca aattgatttt gtttgttatt atttaagtgg
       61 ggtatataat tagagtccgt tatcaatgct aaatattcta atcattatga caggcgaggg
      121 agtgtccaat tatgaattca aaaaagcttt gttgcatatg tgtgttattc tcgctgcttg
      181 caggatgtgc ctctgaatct tctattgatg aaaagaagaa aaaagctcaa gtcacacaaa
      241 gtaatattaa taaaaacact ccccagcaac tgacagacaa agatttattc ggtaatgaaa
      301 ccactctggc cgtatccgaa gaggatattc aagcagcgct tgatggagat gagttccgcg
      361 ttccccttaa ttctcctgta atacttgttc aatccggcaa ccgcgcaccg gaaaccatta
      421 tgcaggagga gatgcgaaaa tattatactg tttccacatt ctctggtatc ccggacaggc
      481 aaaagcctct gacttgtaac aaaaacaaag ataaaaatga gaacgaggat gttgcccgtg
      541 ctgagaatat gaactggatg caggcactgc gttttgtggc tgcaaaagga catcagaaag
      601 cgattattgt ttaccaggat atgttgcaga caggaaaata tgactctgcg ctgaaatcaa
      661 cagtatggtc cgactataaa aatgaaaaac tcactgacgc tatctccctg cgctacctgg
      721 tacgtttcac gctggtggat gtggcaacag gtgagtgggc tacctggtcg ccggtgaatt
      781 atgaatcgag agtaattttt ccgccggctg gaataacaaa aacgagcaat aaagaactat
      841 caaatgacca tgttacggaa gcgcaactgt ttaatttgaa acaaaaaaca tatgtatcga
      901 tggtgaaaga cttggtgagt cgattccagt aatcgttatt gttatgctgc cgcctgtagt
      961 ttagggtggc agcaaacagg agttgagtat ttctataatg taa
//
LOCUS       DAZFBR010000375          979 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFBR010000375 DAZFBR010000000
VERSION     DAZFBR010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.379"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>979
                     /locus_tag="ACHJZ2_005358"
     CDS             <1..>979
                     /locus_tag="ACHJZ2_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571225.1"
                     /translation="SYALIDNASLTAVERTLGDILVKNPDTINGDLVAFENLIQAILF
                     YDTLICVDNYKKEYRDKRIAKFDFIKFVSESDFQLSELDQLAQVESRQITSEIRGGEF
                     VDDDFRQLVEMLKLNMICTWDLRSSVYYLTMKMLGQPGTPEYAKYSELSASIFNELSD
                     ASDTKGYWSTDVKLVSSSGHEYTEREFELEREKSTRGKGGMTRSLEMFIASLNWLAYK
                     SIYYSVIAKHFKADSFIHPIRHAYQLHWMKKTGAFGHDYTARLVQSLSDKISTARSEI
                     VDHGRTSTISLDLPIFSAWLANETGNVSQVINSALELRTHDHFRTCRDVI"
BASE COUNT          310 a          153 c          200 g          316 t
ORIGIN      
        1 gtcttatgcg ttgattgata atgcttcgtt aacagcagtc gaaagaacac tgggtgatat
       61 tcttgttaag aatccagata ctattaacgg agatttagtc gcttttgaaa atcttattca
      121 agcgatactt ttttacgata ccctgatctg tgtagataat tataaaaaag aatatagaga
      181 taaacgcatt gctaaatttg actttattaa atttgtatct gaaagtgatt ttcaactcag
      241 tgaacttgat cagttagcac aagttgaatc tcggcaaatt acttccgaaa ttagaggtgg
      301 ggagtttgtt gacgatgatt ttaggcaact tgtagagatg cttaagctga atatgatttg
      361 cacatgggat cttcggtcta gcgtgtatta cttaacaatg aaaatgcttg gtcagcccgg
      421 tactcctgaa tatgcgaaat atagtgaact aagtgcatcg atttttaatg aattatcaga
      481 tgcttctgat acgaaagggt actggtcaac cgatgtgaag ctagtgagtt catctggaca
      541 tgaatatact gagcgtgaat ttgaactaga aagggaaaaa tctactcgcg gtaaaggagg
      601 tatgactcgt tcacttgaaa tgtttatcgc atcgctaaat tggttggcat ataaatctat
      661 ttattacagc gtcattgcta agcattttaa agcagactca tttattcatc caataaggca
      721 tgcatatcaa ttgcattgga tgaaaaaaac aggtgctttt ggtcatgatt atactgcaag
      781 gttggtgcaa tcgttgtctg ataaaatcag tactgcaaga tctgaaattg tagatcatgg
      841 acgcacatct actatttcat tagatttacc aatattttct gcatggctag caaacgaaac
      901 aggaaatgtc tctcaagtta tcaattcagc tttagagctt agaacacatg atcattttcg
      961 tacgtgtcga gatgtaatc
//
LOCUS       DAZFBR010000376          976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFBR010000376 DAZFBR010000000
VERSION     DAZFBR010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.380"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            34..795
                     /gene="ydeO"
                     /locus_tag="ACHJZ2_005359"
     CDS             34..795
                     /gene="ydeO"
                     /locus_tag="ACHJZ2_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO4571226.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT          301 a          184 c          185 g          306 t
ORIGIN      
        1 atcgataaaa ctttattgtt ttaaggagat aaaatgtcgc tcgtttgttc tgttatattt
       61 attcatcatg ccttcaacgc taacatttta gataaagatt acgccttctc tgacggcgag
      121 atcctgatgg tagataacgc tgttcgtacg cattttgaac cttatgagcg gcattttaaa
      181 gaaatcggat ttaatgaaaa taccattaaa aaatatctac aatgcactaa catccagaca
      241 gtgacgatgc ctgttcctgc gaagttttta cgtgcttcga atgtaccgac tggattgctt
      301 aatgaaatga ttgcttatct caactcggaa gaacgcaatc atcataattt ttcagaactt
      361 ttgctttttt cttgcctgtc tatttttgcc acatgcaaag gtttcattac actattaact
      421 aacggtgtgc tatccgtttc ggggaaagtg agaaatattg tcaacatgaa gctggcgcac
      481 ccatggaagc tgaaagatat ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa
      541 aaactaaagc aagagcaaac gacattctca cagattcttt tagatgccag aatgcagcac
      601 gcaaaaaatt tgatacgcgt agaaggttca gtcaataaaa ttgccgaaca atgtggttat
      661 gccagtacat cttattttat ttatgcgttc cgcaaacatt tcggcaacag tccaaagaga
      721 gtttctaagg agtaccgttg tcaaaggcac acgggtatga atacgggcaa cacgatgaat
      781 gctttagcta tttgattatt tgctaacgag tagtcaacca cgcaccctac gtaaaaatta
      841 aaaatggggc agtcattccc tgccccgcgt tgtttttagg cgatatattt attgaaataa
      901 ataagtgaca ttcatcacat atttatgcat ttgcataact tgttgcatga ttatttatga
      961 tctcaattcg gcattt
//
LOCUS       DAZFBR010000377          974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFBR010000377 DAZFBR010000000
VERSION     DAZFBR010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.381"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>974
                     /locus_tag="ACHJZ2_005360"
     CDS             <1..>974
                     /locus_tag="ACHJZ2_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4571227.1"
                     /translation="ALDRLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDESD
                     RLTHRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNPE
                     TGELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRDR
                     LHRETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWNDNGDLVRISGPRQTR
                     EYGYSATGRLESVRTLASDLDIRIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDA
                     HYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFYTRI"
BASE COUNT          225 a          296 c          320 g          133 t
ORIGIN      
        1 tgcgcttgac cggctggtac agcagcgcgg ctttgacggg cggacgcaac gttaccacta
       61 tgacctgacc ggaaaactca cgcagagtga agatgagggg cttgtcaccc tctggcacta
      121 cgacgaatcg gaccgcctca ctcaccgcac ggtgaacggc gaaccggcag agcagtggca
      181 gtacgacgag cacggctggc tgacagaaat cagccacctg agcgaaggcc atcaggtggc
      241 ggtgcattac ggttatgatg ataagggccg cctggccggg gagcgccaga cggtgcataa
      301 cccggagacg ggggaactgc tgtggcagca tgagacagag cacgcataca acgaacaggg
      361 tctggcaaac cgcgtcacgc cggacagcct gccgcgggtg gagtggctga cctacggcag
      421 cggttatctt gcggggatga agctgggcgg gacgccgctg gtggagttca cgcgcgacag
      481 gctgcaccgc gagacggtgc gcagcttcgg caataacgca tacgaactga ccagcacata
      541 cactcccgca ggccatttac agagccagcg cctgaacagc caggtgtatg accgtgacta
      601 cgactggaat gacaatggcg acctggtgcg catcagcggc ccgcgacaga cgcgggaata
      661 tggctacagc gccacgggca ggctggagag cgtgcgcacc cttgcatcag acctggatat
      721 ccgcatcccg tatgcgaccg acccggcggg aaaccggctg ccggacccgg agctacaccc
      781 ggacagcacg ctcacggcgt ggccggataa ccgcatcgcg gaggatgcgc actatgtcta
      841 ccgacacgat gaatacggca ggctgacgga gaagacggac cgcatcccgg cgggtgtgat
      901 acggacggac gacgagcgga cccaccacta ccactacgac agccagcacc gcctggtgtt
      961 ctacacgcgg atac
//
LOCUS       DAZFBR010000378          973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFBR010000378 DAZFBR010000000
VERSION     DAZFBR010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.382"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>973
                     /locus_tag="ACHJZ2_005361"
     CDS             <1..>973
                     /locus_tag="ACHJZ2_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4571228.1"
                     /translation="LPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHV
                     PEKEAIVDNGAHFDGDQSGTLNSVIPPAVQHLTVEVSAADSQYLAQAKWDTPRVVKGV
                     RFSLRLTSGSGQDSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPAITTF
                     RINAPAKPATIELTPGYFQITAVPVLAVYDPTVQFEFWFSEKRITNTAQVEKSARYLG
                     SGSQWTVQGSRIKPGTDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFREKIGKL
                     HLAQGLWELIDNSQLADEMAEMKTTITETRNEITQTVSKTLENQSAT"
BASE COUNT          229 a          256 c          310 g          178 t
ORIGIN      
        1 ctctgccgga tggcgtggag acatacggtg tgtgggggct ctccctgccg tcactgcgtc
       61 gtcgcctgtt ccgctgtgtc tccatccggg aaaacacgga cggcaccttt gccatcacgg
      121 cagtgcagca cgtaccggaa aaagaagcca tcgtggataa cggggcgcac tttgacggcg
      181 accagagcgg caccctgaac agcgtcatcc ctccggcagt gcagcacctg acggtggagg
      241 tgagtgcagc tgacagccag tatctggcgc aggcgaaatg ggacacgccg cgggtggtga
      301 agggcgtgcg cttcagtctg cgcctgacca gtggaagcgg tcaggacagc cgtctggtga
      361 ccaccgccat cactgcggat acagagcatc gtttcagtgg tctgccgctc ggggaataca
      421 ccctgacagt cagggcaatt aacagttatg gccagcaggg ggaaccggcc atcaccacct
      481 tccggattaa cgcgccagca aaacccgcca ccattgaact gacgccgggg tattttcaga
      541 taacggcggt accggtgctg gcggtgtatg acccgacggt gcagtttgag ttctggtttt
      601 cggaaaaacg catcacgaac acggcacagg tggaaaaatc tgcccgttat ctggggagcg
      661 gcagtcagtg gactgtccag ggaagccgga ttaagccggg gacggatttc tggttttacg
      721 tgcgcagcgt caacctggtg gggaaatctg cgtttgtgga agtcagcggg cagcccagca
      781 atgatggtga agggtatctg gaatttttcc gggaaaaaat aggaaaactg catctggctc
      841 aggggctatg ggagctgata gacaacagcc agcttgcgga tgagatggcg gagatgaaga
      901 ccaccatcac ggaaacccgc aatgaaatca cacagacggt cagtaaaacg ctggagaacc
      961 agagcgccac tat
//
LOCUS       DAZFBR010000379          972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFBR010000379 DAZFBR010000000
VERSION     DAZFBR010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.383"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            258..860
                     /gene="fimB"
                     /locus_tag="ACHJZ2_005362"
     CDS             258..860
                     /gene="fimB"
                     /locus_tag="ACHJZ2_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO4571229.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          291 a          170 c          207 g          304 t
ORIGIN      
        1 tgatgagggt tattattaga ttcgtatccg attgataaat atataaaggt acatagcatg
       61 caagagcata gcgtttgtat ggcaacgtta ttataattaa ctgttgctac tccatttaag
      121 ttcactcaga agaactggtc cacttacgtt agttattaag caaacgttcg cttttataaa
      181 cataatcagg ataaaaatgt tggattattg ctaacctagc acagctagtg cgcgtctgta
      241 attataaggg aaaaacgatg aagaataagg ctgataacaa aaaaaggaac ttcctgaccc
      301 atagtgaaat cgaatcactc cttaaagcag caaataccgg gcctcatgca gcacgtaatt
      361 attgtctgac tttgctttgt tttattcatg gtttccgggc gagtgaaatt tgtcgattga
      421 ggatttcgga tattgatctt aaggcaaagt gtatatatat ccatcgatta aaaaaaggct
      481 tttcaacaac gcacccgcta ttgaacaaag aagttcaggc tttaaaaaac tggttgagta
      541 tccgtacttc ttatccgcat gctgagagcg agtgggtatt tttatcgcgt aaggggaatc
      601 cgctttctcg gcaacaattt taccatatta tctcgacttc cggtggtaat gccgggttgt
      661 cactggagat tcatccgcac atgttacgcc attcgtgtgg ttttgctttg gcgaatatgg
      721 gaatagatac gcgacttatc caggattatc ttgggcatcg caatattcgt catactgtct
      781 ggtatactgc cagcaatgca gggcgttttt acggcatctg ggatagagcc agaggacgac
      841 agcgtcacgc tgttttatag agatagttga gataccaggg atggtgttaa aaactacatt
      901 attcttacga tatatcaaaa atgattaacc atttattaat tgatgttatt gtaagtatga
      961 ttcttaatta tc
//
LOCUS       DAZFBR010000380          968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFBR010000380 DAZFBR010000000
VERSION     DAZFBR010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.384"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..204
                     /locus_tag="ACHJZ2_005363"
     CDS             <1..204
                     /locus_tag="ACHJZ2_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO4571230.1"
                     /translation="YENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLALIS
                     VATCWLLWKYRAILTNLVRLKIK"
     gene            220..801
                     /locus_tag="ACHJZ2_005364"
     CDS             220..801
                     /locus_tag="ACHJZ2_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO4571231.1"
                     /translation="MNLALRKIIYAPISYIHPQRVSLNNTPINNPVLRSITNEMILLQ
                     YNLSVEHFNLNSSLIYYINNWNLLPLICLLSGCHFYRERFAERGFFYKVPDVLRDYLS
                     AIPLEINEKARYKPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHILS
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
     gene            743..>968
                     /locus_tag="ACHJZ2_005365"
     CDS             743..>968
                     /locus_tag="ACHJZ2_005365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571232.1"
                     /translation="MRKKIEMSLIKSPANGVVIKRKISDGLKEIVSLKEKILLETTAK
                     IQSIEEKREEKFIQGYYDGYTKGIIDVMDNF"
BASE COUNT          327 a          156 c          169 g          316 t
ORIGIN      
        1 tatgagaata tttctgttgt attgtcaaaa cgaagagaca ttattgagca agcaccaaca
       61 tatgaaatta gcgaacctgt ttttgcttat gataaggcta tgccggtttc tatcttactt
      121 gcattaatat ccgtagcgac ttgctggctg ttatggaagt atagggccat tctaactaat
      181 ttagtcaggt tgaaaattaa gtagacatat gagaacgtta tgaatcttgc cctaagaaag
      241 attatatatg ctccaattag ttatattcat cctcaacgag taagcttgaa taatacacct
      301 attaacaatc cggtgctgag atctattacc aatgaaatga ttctcctgca gtataatctt
      361 tcagttgaac attttaatct gaatagttca cttatttact acataaacaa ttggaattta
      421 cttccattga tctgtttact cagtggttgt cacttttatc gagaaaggtt tgcagaacgc
      481 gggttttttt ataaggttcc tgatgtacta agggattatt tatcagctat accgctggaa
      541 ataaatgaaa aggcacgata caaacccggc atcgctaact atcacaatat catcacatgt
      601 ggtttttcaa cgttgttacc ctatataagg caacaaccat tggcaatgca gcaacgattt
      661 aatttattat ttcctgattt tgttgaccac atactatcgc cgttaccact tgcatcaaca
      721 cttttagaga gaattacatt ttatgcgaaa aaaaatagag atgagcttga taaaatctcc
      781 tgcaaatggt gttgtgatta aaagaaaaat ttctgatgga ttgaaagaaa tagtttctct
      841 taaagaaaaa atccttcttg aaaccaccgc aaaaattcag agtattgagg aaaagagaga
      901 agaaaaattt attcaaggtt attatgatgg ctatacaaaa ggcattattg atgtgatgga
      961 taatttta
//
LOCUS       DAZFBR010000381          955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFBR010000381 DAZFBR010000000
VERSION     DAZFBR010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.385"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            1..607
                     /locus_tag="ACHJZ2_005366"
                     /pseudo
     CDS             1..607
                     /locus_tag="ACHJZ2_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308318.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-three component system protein"
     gene            607..>955
                     /locus_tag="ACHJZ2_005367"
     CDS             607..>955
                     /locus_tag="ACHJZ2_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFO4571233.1"
                     /translation="MVRIYNSSLEVACRMAKVLVAIYPSSLSLERLICFDFILVNLKD
                     FLPEEISLHPPIPRRDAQLALKREIVLESLALLQGYELASKIYTHRGFVYKASEKTYA
                     FTNSLHNEYVAQME"
BASE COUNT          316 a          145 c          161 g          333 t
ORIGIN      
        1 gcaatttcaa aaaaataact aaaaagattg atattccatt agatgatgaa ataaaaaata
       61 tattgaggat tttgatttta gtatttttta ctctctaccc ttatcattga ttttaaatga
      121 tattgcaaat acacaccttt attttaagta ctttaacgag ctgtatgtcg tgagaatccc
      181 tccaaatgaa attccaacat acaattcaaa aaaagagtct gtatatgtta atgcactgct
      241 tcaagcctat tcagagcatg gaaataaaac ttatagttct ttcttagagc ttgatgatcc
      301 atacagacga cactttaata atagtagaaa tgatttttat tttgcatctt cgcttgaggt
      361 ttttgtccgc gaagtattta aagatgatgt attcaaagca ttgaaatgtt acatttcatc
      421 ttcaattgaa cccgtctttt atgaagacca taattatgca tttattaggt gtaatgcagt
      481 cttgaagcag gctgttctga caccaattgc acattcagta ctatcaaaaa tatgtgaagc
      541 aaatgataaa aaaggaatat gccatcattt ggttaatgat ggtgaagtaa tttggacggt
      601 gagataatgg ttagaattta taattcaagt ttagaagtgg catgtcgaat ggcgaaagtg
      661 ctcgtcgcta tttatccttc ttcattaagc cttgaacggc ttatttgttt tgattttatt
      721 ttagtaaatc ttaaggattt tttacctgaa gagattagtc ttcatcctcc aataccccgt
      781 agagatgctc agttagccct aaaacgagag attgttttag aatcattggc tttgttgcaa
      841 ggctatgaac tagcctcaaa aatttataca catcgtggtt ttgtatataa agcttctgaa
      901 aaaacatatg catttacaaa ttctctacat aatgaatatg ttgcgcagat ggagc
//
LOCUS       DAZFBR010000382          949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFBR010000382 DAZFBR010000000
VERSION     DAZFBR010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.386"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..381)
                     /locus_tag="ACHJZ2_005368"
     CDS             complement(<1..381)
                     /locus_tag="ACHJZ2_005368"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO4571234.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLH"
     gene            complement(445..>949)
                     /locus_tag="ACHJZ2_005369"
     CDS             complement(445..>949)
                     /locus_tag="ACHJZ2_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO4571235.1"
                     /translation="HETAVVRRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRI
                     HEAELLDHIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVL
                     VHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKK
                     EYAVFFI"
BASE COUNT          205 a          316 c          236 g          192 t
ORIGIN      
        1 atgcagtccg cctccgtcca gaaaggtgta ttcatgggga cggaagccca cggacagccc
       61 cctgaccagc ccggtcttaa tggccgccca gacctcatcc agccgggcag ccagttgcga
      121 cggcatatcc ggtacgggct tcaccagtgt tgccgtgatt tccagccctt cgctgacccg
      181 gcgcaccgta cactgcccca ccgggcggga atggtcatgc tgccagagaa acgggatcgc
      241 actgccaaac tccgcgccct ccggctccag gatgtcacca tcccgatccg gagaaggcgt
      301 tgacgcaatc ccggtgatca cccgttcatc ctcactgaag gatttcaccg tcagcaggga
      361 acaggcccgt ttaagagtca catcagcctc ctgaaaataa aaaaaccgcc gcagcggttc
      421 atgatggtta cagtgtgaac agggttatat gaaaaaaacc gcatattctt tctttttcgg
      481 ttccgggtta agggacatca gggagaccgc attgaacagc gccatcagcg ggtcaatttt
      541 tccccgtcca ctggcctgtt tggtgataag aatggcgtta cctttaggct ccacccgggc
      601 attgccgaca caccaggcca tcaggggctg gtcaccatgc accagcaccc cttcagccag
      661 tttgcgctcg gtggttttaa tggccccgcc cagtttccag ccctggctta tccccaccac
      721 aattccgtcg gggatcccgg cttccgccag tgaatccaga atctgcccca cccctgacgg
      781 gtcaataccg atatggtcca gtaactcagc ctcatgaatg cgacgcacat attccgccac
      841 ttccgccgtg tcatccccga cacgccggac aatggtcata tctccacagg caacaagatc
      901 ctgaaaccgg gacgcctcgc tcttccgtcg gaccaccgcg gtttcatgc
//
LOCUS       DAZFBR010000383          944 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFBR010000383 DAZFBR010000000
VERSION     DAZFBR010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.387"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(468..>944)
                     /locus_tag="ACHJZ2_005370"
     CDS             complement(468..>944)
                     /locus_tag="ACHJZ2_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFO4571236.1"
                     /translation="FIEALNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMI
                     MGSHVLKDMPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKT
                     DPQVFGSVYYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          346 a          206 c          117 g          275 t
ORIGIN      
        1 aattcataaa atatgattct ccacgatttg cccagaaata acgaacataa ttttcgttgt
       61 aattaaaatc atatttcatc tatagtgacc attcacagca aaacaaaatc aattatcaca
      121 ttcaaaaaaa tactgacaac aaagatactc ttcagacaaa gttgtcatat gtaaattttc
      181 ccttaaaacg ttgctcaaga aaaatataaa aatcatatgc cctaatcaac atcctcaagc
      241 actacgatat atgcagtatc ctattcatat attaccacca tatcagacaa atcgaatcac
      301 aacgagttct atcgtactct ttcatcttcg acactgtaaa ttttaagaac attcggctca
      361 tgccactaaa ccaagacata ataacaacgg agcaataaat gttcccactg aacatgtgca
      421 aacctaaaaa gacagtgtta caataatatt catccatggt ctctaaatca acgccacgca
      481 acaggataat acaatgaaaa tatttcagga aaagattctt ttgcatgctc cgttagcata
      541 tcagtcagca cagcggtaca agtaaaaaca ttatttcttc cttgtagcct gtctaaaata
      601 tcattgtaat aataaactga gccaaatacc tgaggatctg ttttataagc ttgttcaatc
      661 aatccatttg catatctccg tagaatctca ggagaatcat actctgtacc aaacatatca
      721 ctcgatgagt atttacagaa aaccccccct aaacaaaata atatttcagc ctgttttctt
      781 agtggcatat ctttcaatac atggctcccc ataatcatat ctacatgctg agcgttcagt
      841 tcccaaccat caagaaaggg attccagaca caggcaagtt tcctttgctg atcatcagca
      901 atcattttat aatctgattt attggaatta agtgcctcaa taaa
//
LOCUS       DAZFBR010000384          932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFBR010000384 DAZFBR010000000
VERSION     DAZFBR010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.388"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            1..932
                     /locus_tag="ACHJZ2_005371"
                     /pseudo
     CDS             1..932
                     /locus_tag="ACHJZ2_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
     misc_feature    69..185
                     /locus_tag="ACHJZ2_005371"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          227 a          219 c          287 g          199 t
ORIGIN      
        1 cgtctgaaag agctggaacg tgaaaatcgt gaactgcgcc gcagtaacga tatccttcgc
       61 caggcttccg cttattttgc gaaggcggag ttcgaccgcc tctggaaaaa atgatgccac
      121 tgctggataa gctgcgtgag cagtacgggg tcggaccggt atgcagcgaa ctgcatattg
      181 ccccgtcaac gtattaccat tgtcagcaac agcgacatca tccggataaa cgcagtgccc
      241 gtgcgcagca cgacgactgg ctgaagagag agatacagcg cgtatacgat gaaaatcatc
      301 aggtgtacgg tgtgcgtaaa gtctggcgtc agttgttacg ggaaggaatc agggtggcca
      361 gatgtacagt ggcacgtctc atggcggtta tgggacttgc cggtgttctc cggggtaaaa
      421 aggtccgtac gaccatcagc cggaaagccg ttgccgcagg cgaccgcgta aaccgtcagt
      481 tcgtggcaga acgacctgac cagctgtggg tggctgattt tacttacgtc agcacatggc
      541 agggcttcgt ctatgtggcg tttatcattg atgtgtttgc cggatacatc gtggggtggc
      601 gggtctcatc gtctatggaa acgacattcg tgctggatgc gctggagcag gcgttgtggg
      661 cccgtcgtcc gtctggcacc atccatcaca gcgataaagg ctctcagtat gtgtcactgg
      721 cctatacgga gcgactaaaa gaagccggat tactggcatc aacagggagt acaggcgact
      781 cgtatgacaa cgcgatggct gagagcatca atggtcttta caaagcggag gtaatacacc
      841 gtaagagctg gaaaaaccgt gcagaagtgg aactggccac actaacgtgg gtggactggt
      901 ataacaatcg acgattgctg ggaaggctgg gc
//
LOCUS       DAZFBR010000385          924 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFBR010000385 DAZFBR010000000
VERSION     DAZFBR010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.389"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..877
                     /locus_tag="ACHJZ2_005372"
     CDS             <1..877
                     /locus_tag="ACHJZ2_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFO4571237.1"
                     /translation="TTLSNSSDSDQTVQAVRLPDVSPALVSKVTDAVMEQVVEWQNRP
                     LDAVYPIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLN
                     VLTELKNRGLNDILIACVDGLKGFPDAINTVYPEARIQLCIVHMVRNSLRFVSWKDYK
                     AVTRDLKAIYQAPTEEAGQQALEAFASAWDSRYPQISRSWQANWTNLAMFFAYPADIR
                     KVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQAASQKWTMPLRDWRMAM
                     SRFIIEFGDRLDGHF"
BASE COUNT          228 a          247 c          252 g          197 t
ORIGIN      
        1 caccactttg tctaattcgt cagattctga ccagacggtt caggctgtac gcttacctga
       61 tgtctcaccg gcgctggtat cgaaggtcac cgatgccgtt atggagcagg tagtcgaatg
      121 gcaaaaccgg ccactggatg cggtctatcc cattgtttat cttgactgta ttgtcctgaa
      181 ggttcggcag gacagtcgcg taatcaataa atccgtattc ctcgcactgg gtatcaatat
      241 cgaaggccag aaagagttac tgggtatgtg gctggccgaa aacgagggag cgaagttctg
      301 gctcaatgtg ctgactgaac taaaaaaccg cggcctgaac gatattctca tagcctgtgt
      361 cgacgggctg aaaggcttcc cggacgctat caacacggtg tatccggaag cccgcatcca
      421 gctgtgcatc gtgcatatgg tgcgcaacag cctgcggttc gtttcctgga aggactacaa
      481 agccgtcacc cgcgacctga aagccatcta ccaggccccc acagaagaag caggccagca
      541 ggcgctggaa gcgttcgcca gcgcctggga cagtcgctac ccgcagataa gccggagctg
      601 gcaggcaaac tggaccaacc tggcgatgtt cttcgcttac ccggcagata tccgcaaagt
      661 catctacaca accaacgcca tcgagtcgct gaacagcgta atccggcatg ccatcaaaaa
      721 acgcaaggtg ttcccgacgg atgactcagt gaaaaaggtc gtgtggctgg caatccaggc
      781 ggcttcacag aaatggacga tgccactgcg ggactggcgt atggcaatga gccgctttat
      841 tatcgagttc ggtgaccgcc tggacggtca cttctgagaa aaggcattta cacagaatcg
      901 tgtacagggt cgattttact cgtt
//
LOCUS       DAZFBR010000386          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFBR010000386 DAZFBR010000000
VERSION     DAZFBR010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.390"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..133)
                     /locus_tag="ACHJZ2_005373"
     CDS             complement(<1..133)
                     /locus_tag="ACHJZ2_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone protein"
                     /protein_id="HFO4571238.1"
                     /translation="MPMYLLRLFFAVTLITQMSMATAGIVVGGTRVIFYGDKPDTTIS
                     "
     gene            complement(237..839)
                     /gene="lpfA2"
                     /locus_tag="ACHJZ2_005374"
     CDS             complement(237..839)
                     /gene="lpfA2"
                     /locus_tag="ACHJZ2_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFO4571239.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
BASE COUNT          287 a          197 c          174 g          258 t
ORIGIN      
        1 tagagattgt ggtatcaggt ttgtcaccat aaaaaataac gcgagttccc ccaacaacaa
       61 taccagccgt agccatcgac atctgcgtaa tcaaagtaac agcaaaaaac aatctaagta
      121 aatacatagg cataaatgta tctcataaaa aatataataa tgacgccctt catttaagta
      181 aaagagaacc ataatggcga gcgccatttt atttaaggta ttatgttaag aatgatttat
      241 tttatatatt caactgtaaa ctgcgagtcg gcgttagctg taccagggtc aatagtcgtt
      301 tccgtagcaa tataacgagc catgaacttc atggatgcat taccattaga aatcggagtt
      361 gatgcagaat tttcatataa cttgacctga gtgttatctg cacggttata taaacgaata
      421 gctacgcctt tcgctgcact aacggcaccc gcaccagtat aatccccact gttatcactc
      481 gggctaatac cagcggcagc atcgttagag ttatccagcg gggtaccaat tgccaggttg
      541 ccattaccat gagcatcttc attaccatcg aaacgaattg cggcggactt aaaggtttcc
      601 gggcattgag tcagatcgat agaaaaagcc gtcggggcag ccacatcatc gacgccacta
      661 aaggctgtac ggtttacttt gcctaaatca acgacctgat ctttagtttc aggagtgact
      721 tcgcaagtac tgtcaataat ttcaccacgg aaatggactg ttccatcagc cgcctgtagg
      781 tgacctgcca taaacacaga agttaacgct aatgctccta caatcatatt tggtttcata
      841 aatataactc cattaatttg atattattcc tgtgtgttat ccatttatta aatttggata
      901 aacctgatcg gtattt
//
LOCUS       DAZFBR010000387          907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFBR010000387 DAZFBR010000000
VERSION     DAZFBR010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.391"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            66..281
                     /locus_tag="ACHJZ2_005375"
     CDS             66..281
                     /locus_tag="ACHJZ2_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4571240.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            380..601
                     /locus_tag="ACHJZ2_005376"
     CDS             380..601
                     /locus_tag="ACHJZ2_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4571241.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            598..>907
                     /locus_tag="ACHJZ2_005377"
     CDS             598..>907
                     /locus_tag="ACHJZ2_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4571242.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESDAQAYANAEFIAEANPATVLELLDERERNQQYIKRRDQEN
                     E"
BASE COUNT          281 a          194 c          226 g          206 t
ORIGIN      
        1 tgcatttttc tggcagcagg cttcatattc tgtgtgctta tgcttgccga catgggactt
       61 gttcaatgac acctcagcag gaaaacgccc ttcgcagcat tgcccgtcag gctaattctg
      121 aaatcaaaaa agccagacag cagtttccgg ataaaaacgt cgatgacatt tgccgtagcg
      181 tactgaagaa gcaccgcgaa acggtaacgc taatgggatt cacaccgact catttaagtc
      241 tggcgatcgg catgttaaac ggcgtcttta aggagcgatg aacatgaaaa gcaaaatcat
      301 cagggagcta caggctcctt ttttattgtt cgcattcacc ctcaagcgta ttaaccaaca
      361 attcagggat taatgaaaga tggcagacat cattgattca gcatcagaaa ttgaagaatt
      421 acagcgcaat acagcaataa aaatgcgtcg cctgaactac cagactgtat ccgcaactca
      481 ttgttgtgag tgtggcgatc cgatagatga acgaagacgc ctggcagttc agggttgtcg
      541 gacttgtgca agttgccagg aggatctgga gcttatcagt aaacagagag gttcgaagtg
      601 agcgaaatta attatcaggc actgcgtgaa aaggcagaaa aagcaactaa aggaagctac
      661 atcgtagggc atacatctgt taaccagcac ggcaatttaa caggagtttt tgtttgccaa
      721 aaatggaaag gagaacccgg tggcgtgatt gcggaatgtc atgttaactg cctgattgaa
      781 tcagatgctc aggcttatgc aaacgctgaa ttcatagcag aggctaaccc ggctaccgtg
      841 ctggaactgc tggatgaacg ggaaagaaac cagcaataca tcaaacgccg cgaccaggag
      901 aacgagg
//
LOCUS       DAZFBR010000388          907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFBR010000388 DAZFBR010000000
VERSION     DAZFBR010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.392"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..588)
                     /gene="fimE"
                     /locus_tag="ACHJZ2_005378"
     CDS             complement(<1..588)
                     /gene="fimE"
                     /locus_tag="ACHJZ2_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO4571243.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKRE"
BASE COUNT          290 a          193 c          173 g          251 t
ORIGIN      
        1 ttctcttttt aatttttcgt ttatgagatt atttctttcc cataatccgg caaaacgagc
       61 agcattactg gcggtataac gtacagtatg gcgaatattt cgatgcccga gataatcctg
      121 aattaaacga gtatctgcac cacgctccgc cagttcataa ccgcaagcat gccttaacat
      181 atgaggatga gtctgcgtta cggttccagc ttcaataccg gcatcacgaa taatgcgata
      241 ggcctgctgg cgagaaagcc gactcccgcg gcgagaaata aatatggcgt cggtccggtc
      301 agcgcctttc cagttagcac gttctagggt ccagcgttcc acagcttcac gctcatcaaa
      361 acgtaacggg tgaacggtag aaaatccgtt cttcagtcgg cgaatattta ttcggccttc
      421 attaaggtca agatcctgat aatgcagatc aagcagttca ctaatacgca tcccatgccg
      481 atatgccaac agaataagac aataatctct ggctcccgtt gccccgtaac aaaccgcctg
      541 catcatggcc tgaacttctt taccggtaag ataacgacgt ttactcacaa tagtagtact
      601 cctaactgag atatatttga atgaatacct ataggaaacc tcaatcggtc aaaattagcc
      661 caatagataa aaagataacc acacacgaag tgatgtggct atcagtcaat tacattaaat
      721 atcatacaaa aataaaatat cactgatgtg ataacatcta ttttcattga tttgattcaa
      781 tataacaata gattgcagtg gattaacttc cagttgctta aaaaataaag gaaaaagtgc
      841 agaaacagga gagtttaagt gatgtgtaaa tttatgaatg attttgttta tttattttga
      901 agaacat
//
LOCUS       DAZFBR010000389          902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFBR010000389 DAZFBR010000000
VERSION     DAZFBR010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.393"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            1..464
                     /locus_tag="ACHJZ2_005379"
                     /pseudo
     CDS             1..464
                     /locus_tag="ACHJZ2_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            461..787
                     /locus_tag="ACHJZ2_005380"
     CDS             461..787
                     /locus_tag="ACHJZ2_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4571244.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVISEDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            797..>902
                     /locus_tag="ACHJZ2_005381"
     CDS             797..>902
                     /locus_tag="ACHJZ2_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571245.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCG"
BASE COUNT          198 a          208 c          307 g          189 t
ORIGIN      
        1 ctggaggcgg tgagtgccct gccggctccg caggacgggc gtgatgccac ggcactggaa
       61 atactccccg ccattgacga tcaaaaatcc tttccccggg gctcgtatgc cacacaccag
      121 ggtggactct ggcgggcgta tgaaaaaacg tacgggatgc ggggatggga atgcctggtt
      181 gacggggtgg cggatattga cgtcagcatg acgggtgaac ggtcgttctc tgtggtggtc
      241 cggcagagca gtggccagcg tacggaaaaa acattttccc tgccggtgat gctctaccgt
      301 ggtgtgttca gaatcggcga aacttaccac cccggcgata cggtgacgtg gggggctcgt
      361 tgtggcactg caacagtatg accggtgata agcccggaga agctcattca tcaggctgga
      421 ctctggctgc aaaacgtggg cgggatgcaa gaggtggaaa gtgacagcat tactgacact
      481 ggaagaaatc aaggcacatc tgcgtgttga ccatgacgcg gatgatgaga tgctgatgga
      541 caaggttcgt caggctaccg ccgtgctgct ggcctacatc cagggcagcc gggataaagt
      601 gattagtgag gacggtgagc tgatccctgg tgaggcatta acccggatga agggggctgc
      661 catgcgactg accgggatgc tgtaccggaa tccggatctt gctgagcggg aagacctcgt
      721 gcagggggaa ctgccgtttt ctgtttccgt gctgatttac gatttgcgtt gtccgacggt
      781 gttatgagga gggggaatgg caatatctgc aggtcgtctg acacagatga tcagtgttct
      841 gaacccggtg ttaacccgta atgctgccgg agaaatgacg gaagaatggg tgtcatgcgg
      901 ga
//
LOCUS       DAZFBR010000390          899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFBR010000390 DAZFBR010000000
VERSION     DAZFBR010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.394"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            154..336
                     /locus_tag="ACHJZ2_005382"
     CDS             154..336
                     /locus_tag="ACHJZ2_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO4571246.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
     gene            415..>899
                     /locus_tag="ACHJZ2_005383"
     CDS             415..>899
                     /locus_tag="ACHJZ2_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4571247.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKLTDLAFNNVNK
                     KA"
BASE COUNT          307 a          164 c          193 g          235 t
ORIGIN      
        1 actcactgag tacacatttt tacaattgct atttctcaca ttaagatgcg cataccaatc
       61 tcttatttga cactttctta tttgttacta gagttaattg ggtgctgaca aggcacatgt
      121 tatttacaat ttgaaaatca gaggagaaat gatatgaccg aacatcgtgg cggtgcaggt
      181 aatttcgctg aagatcgaca aaaagcttct gacgctggtc gtaaaggcgg ccaacatagt
      241 ggcggtaatt ttaaaaacga tccacaaaga gcatcagaag cagggaaaaa aggtggtcag
      301 aatagccatg gtggcggccg taaatctgac tcttaattta tcacccccta cattcgtgaa
      361 taactgaagc taactgcgag cctacgggct cgcagtgaaa actctggagt gattatgcaa
      421 attaaatctg ttgaagatat cttcatccac ttattatcgg atacatacag cgctgagaaa
      481 caactaacaa aggcactgtc taaacttagt cgctctgcat atagtgataa attaaccgct
      541 gcttttcagt cacatcttga tgagacacat ggtcaaattg aacgcatcga tcaggttgtt
      601 gattctgaag atggtcttaa attgaaaaga attaagtgtg ccgcaatgga aggacttatc
      661 gaagaggcga atgaggtaat tgaaagcact gataaaaatg aagtacgtga tgctgcattg
      721 attgctgcag cacaaaaagt agagcactat gaaattgcca gttatggcac tttggtaaca
      781 ctcgctgagc aattaggata taaaaaagcg gctaaactcc tgaaagaaac actggaagag
      841 gaaaaagcta ctgatgtcaa attaacagac ctggcattta ataacgttaa taaaaaagc
//
LOCUS       DAZFBR010000391          896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFBR010000391 DAZFBR010000000
VERSION     DAZFBR010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.395"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..140)
                     /locus_tag="ACHJZ2_005384"
     CDS             complement(<1..140)
                     /locus_tag="ACHJZ2_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4571248.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQG"
     gene            complement(121..534)
                     /locus_tag="ACHJZ2_005385"
     CDS             complement(121..534)
                     /locus_tag="ACHJZ2_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4571249.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            complement(561..>896)
                     /locus_tag="ACHJZ2_005386"
     CDS             complement(561..>896)
                     /locus_tag="ACHJZ2_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4571250.1"
                     /translation="KFVSDAEQQETTKHDVVHINQRYLETASLLVAMSLWHSHSLKGT
                     LASPETEMQQIRREVMLGWPADALNQATNRVLYLSGMLDNRHDADPEQTGKAEATESV
                     TSKKHSKAS"
BASE COUNT          201 a          276 c          209 g          210 t
ORIGIN      
        1 ccctgttcaa cagtactggc ggtttttctg gcatccgcct ccagactgga aaaatgacgg
       61 cgtacccggg ccatctgttc atcaaaccgg gccgcatcca gactcaaatc aataaccaga
      121 tcacccgctg gctgggacat atctcacacc tccggaaatc cccgctgaag ccatcattaa
      181 tgcaacatca tcctcgctga catccaccac atccgcagaa ggtgaaatat cgcgccctcc
      241 gtcccctccg aaccggacgc ctccggcaac tcctgtcgct ttctgcatca gcatttcttc
      301 ctcgtccggc atctccgtct gctcttcctc acacgctgga gcaagcagac tgaaatccgc
      361 cggatgcata tccggatcgc caaaaaacag gctgagtacg gcgtacatca gcccggaaaa
      421 atgagcgtcc agttgggtat cctgaaaata atgcgtgcag taaaaacgtc gccagtcggc
      481 atattcggtg gatgtcatcc cggcaagcat ggcgcgccag tcgggtctcc ccatctctcg
      541 cgccagtttc aggacaaagt tcagctcgcc ttcgaatgct tttttgatgt taccgactca
      601 gtcgcttctg ctttaccggt ttgttcagga tcggcatcgt gccggttatc cagcatacct
      661 gaaagataaa gcacccggtt cgttgcctga ttcagtgcat cagcaggcca tcccagcatc
      721 acttcacggc ggatctgctg catctctgtc tccggagagg ccagagtgcc tttgagggaa
      781 tgggaatgcc atagcgacat cgccacaagc agggatgccg tttccagata tcgctggtta
      841 atgtgcacga catcatgctt cgttgtctcc tgttgttctg cgtctgaaac aaactt
//
LOCUS       DAZFBR010000392          889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFBR010000392 DAZFBR010000000
VERSION     DAZFBR010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.396"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(462..>889)
                     /locus_tag="ACHJZ2_005387"
     CDS             complement(462..>889)
                     /locus_tag="ACHJZ2_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO4571251.1"
                     /translation="KSTVSVFYLQPEEVNTLSHQAKRGDGEAGFRLFLYYKLSNYDEE
                     KSSQWLKIAANNGHATAQYRLYVELQEQGETKEANEWLQKIKLAASNNDSSAIWFLVN
                     CYKNQGNKKEALQWAYKLKASKQPLDADRLIEELTKEQK"
BASE COUNT          244 a          178 c          146 g          321 t
ORIGIN      
        1 agcaaacaaa aaacaataag catgttgatt gaatatttta aatacatgtt cttttacaat
       61 aaaaaaatga atggttatcg aacaagccag caataataac cttattattt tcataagctc
      121 catccactaa aatgctggag atataaaaaa tgaaccaaac ggcttctttg gcccatactt
      181 aggtttgggg tgtagaatct tagtcataag atctatgccg agtcatgtgc tggccacata
      241 cttaatgata caaaaagcta tattttcatc ttttctcttg cgacttttga tgcatctgcc
      301 aagtacatcg taatgataac cggcgacgaa gatgccagtt ttggtttcaa cttgcgcttt
      361 gatgagcttg ttgcagtcgt cgtaaacgta attttgtgtg taggtgttgt agcgactggt
      421 aaagtttttc tactctccat ttttctgtta cctgtttaaa gctacttttg ttctttcgtt
      481 aattcttcaa ttagtcgatc tgcatcaagc ggctgtttac tcgctttgag tttatatgcc
      541 cattgcaatg cttctttttt atttccctgg tttttataac aattcaccaa gaaccatatt
      601 gctgaactgt cattattact ggcagccaat ttaatttttt gtaaccattc attagcttcc
      661 ttggtttcac cttgttcttg taactctaca tataaccgat actgagctgt cgcatgacca
      721 ttattagccg ctatttttaa ccactgacta cttttttctt catcataatt ggacaacttg
      781 tagtacaaga acaacctaaa acccgcctcg ccatcgcctc gttttgcctg gtgtgataaa
      841 gtatttactt cttctggctg caaataaaag acgcttactg ttgattttg
//
LOCUS       DAZFBR010000393          887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFBR010000393 DAZFBR010000000
VERSION     DAZFBR010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.397"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..173)
                     /locus_tag="ACHJZ2_005388"
     CDS             complement(<1..173)
                     /locus_tag="ACHJZ2_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571252.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQ"
     gene            complement(223..525)
                     /locus_tag="ACHJZ2_005389"
                     /pseudo
     CDS             complement(223..525)
                     /locus_tag="ACHJZ2_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(552..>887)
                     /locus_tag="ACHJZ2_005390"
     CDS             complement(552..>887)
                     /locus_tag="ACHJZ2_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4571253.1"
                     /translation="KFVSDAEQQKTTKHDVVRINQRYLETASLLVAMSLWHSHSLKGT
                     LASPETEMQQIRREVMLGWPADALNQATNRVLYLSGMLDNRHDADPEPTGKTEATEPV
                     TSKKHSKAS"
BASE COUNT          195 a          270 c          206 g          216 t
ORIGIN      
        1 ctctgctcag cagcatcagc ctgttttctg aaagactgac tgactttccc ggacatcaaa
       61 tcagcaagcg cactggtttc acccagctct ttttttaccc gcgctgcctc ttcagaaaaa
      121 cgggcagaat ccagtgtcag cacggctgtc agatcggcaa aattacctgc catcgtggtc
      181 acctcctgaa atgtcctctg ataccatcaa taactgtcac aacctccttc cctcctcccc
      241 gaaacggact ccaccggcga ggcccgccgc cttctgcatc agtacatcat tttcgtccgg
      301 cgtctccgcc tgtccttcct ctgcctccgg agcgaacagg ctgaaatccg ccggatgcat
      361 atccggatcg caaaaaaaca ggctgagtac ggcgtacatc agcccggaaa aatgcatatc
      421 cagctgggta tcctgaaaat aatgcgtgca gtaaaaacgt cgccagtcgg catattcggt
      481 ggatgtcatc ccggcaagca tggcgcgcca gtcggctctc cccatctcac gcgccagttt
      541 caggacaaag ttcagctcgc cttcgaatgc ttttttgatg ttaccggctc agtcgcttct
      601 gttttcccgg ttggttcagg atcggcatcg tgccggttat ccagcatacc tgaaagataa
      661 agcacccggt tcgttgcctg attcagtgca tcagcaggcc atcccagcat cacttcacgg
      721 cggatctgct gcatctctgt ctccggagag gccagagtgc ctttgaggga atgggaatgc
      781 catagcgaca tcgccacaag cagggatgcc gtttccagat atcgctggtt aatgcgcacg
      841 acatcatgct tcgttgtctt ctgttgttct gcgtctgaaa caaactt
//
LOCUS       DAZFBR010000394          885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFBR010000394 DAZFBR010000000
VERSION     DAZFBR010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.398"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>885)
                     /locus_tag="ACHJZ2_005391"
     CDS             complement(<1..>885)
                     /locus_tag="ACHJZ2_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4571254.1"
                     /translation="SPITVTIERKDGYYFINFSGDKSDIIYKVDGFSIKERNFFTLLS
                     GNFKPDWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPQNANTAYFGDTDGRV
                     GAVLYALLVSGHIGIREKGWSLLCDLLKHEDMASFAYENKKLKKLFTLLDKRDMILNE
                     LHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRINKNVV
                     VLAGNHEINFNGNYSARLANHKLSSGDTYNLIKTLDVCNYDSERHVLTSHHGIIRDEE
                     NKCYCLGALQVPFNQMKNP"
BASE COUNT          290 a          178 c          131 g          286 t
ORIGIN      
        1 ggatttttca tctgattaaa tggaacctgc aaagcaccga gacaataaca tttattttct
       61 tcatccctta ttattccgtg atgactggtt aaaacatgcc tttctgagtc ataattacaa
      121 acatcgagcg ttttaattaa gttatatgta tcaccggaag agagtttatg atttgctagt
      181 cgtgcactat agttaccatt aaaattgatt tcatgatttc cagccagaac aacgacattt
      241 ttatttatac gactatcttt attaccctcc atatttcgca tagaatttag caaagtaagg
      301 atatatttat caccaaaaat agtactgaat ctatcgcctg tatgatcccc aaggaagata
      361 caaggggtaa tcgcatcgcc ttttaaaaaa acatgttgat gcaactcatt caagatcatg
      421 tctctcttat ctaataaagt gaagagtttt ttcaattttt tattttcata tgcgaatgaa
      481 gccatgtctt cgtgtttcaa taaatcacat aataaactcc accccttttc cctgatgcct
      541 atatgccctg aaacgagcaa tgcataaagt acagcgccta ctcttccatc tgtatcgcca
      601 aaataagccg tgttggcatt ttgtgggtta atcacaccac actgtttata aaagtctatt
      661 tttgaaaaaa cagattcaac atagctatca aacttttcct tagtaaacgt ctctttagag
      721 acagcccaac gccagtcagg tttgaaatta ccactaagaa gagtaaagaa atttctctct
      781 ttgatagaaa acccatcaac tttataaatt atgtctgatt tatccccgga gaaattaata
      841 aaataataac cgtccttacg ctcaatcgta acggtaatgg gagac
//
LOCUS       DAZFBR010000395          868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFBR010000395 DAZFBR010000000
VERSION     DAZFBR010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.400"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..465
                     /locus_tag="ACHJZ2_005392"
     CDS             <1..465
                     /locus_tag="ACHJZ2_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4571255.1"
                     /translation="PIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTL
                     TPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALD
                     CFVYALAALRISISRWQLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            462..668
                     /locus_tag="ACHJZ2_005393"
     CDS             462..668
                     /locus_tag="ACHJZ2_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO4571256.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            665..>868
                     /locus_tag="ACHJZ2_005394"
     CDS             665..>868
                     /locus_tag="ACHJZ2_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4571257.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNG"
BASE COUNT          225 a          223 c          262 g          158 t
ORIGIN      
        1 cccattaaag gggcatccgt ctacggaaag ccggtggcca gcatgccacg taagcgaaac
       61 aaaaacgggg tttaccttac cgaaatcggt acggataccg cgaaagagca gatttataac
      121 cgcttcacac tgacgccgga aggggatgaa ccgcttcccg gtgccgttca cttcccgaat
      181 aacccggata tttttgatct gaccgaagcg cagcaactga ctgctgaaga gcaggtcgaa
      241 aaatgggtgg atggcaggaa aaaaatactg tgggacagca aaaagcgacg caatgaggcg
      301 ctcgactgct tcgtttatgc gctggcggcg ctgcgcatca gtatttcccg ctggcagctg
      361 gatctcagtg cactgctggc gagcctgcag gaagaggatg gtgcagcaac caacaagaaa
      421 acactggcag aatacgcccg tgccttatcc ggagaggatg aatgacgcga caggaagaac
      481 ttgccgctgc ccgtgcggca ctgcatgacc tgatgacagg aaaacgggtg gcaacggtac
      541 agaaagacgg acggcgagtg gagtttacga ccacttccgt gtctgacctg aaaaaataca
      601 ttgctgagct ggaagtgcag accggcatga cacagcgacg caggggacct gcaggatttt
      661 atgtatgaaa atgtccacca ttcccaccct tctggggccg gacggcatga catcgctgcg
      721 tgaatatgcc ggttatcacg gcggtggcag cggatttggt gggcagttgc gggcgtggaa
      781 cccaccgggt gaaagtgtgg atgcagccct gctgcccaac tttacccgtg gcaatgcccg
      841 cgcagacgat ctggtacgca ataacggc
//
LOCUS       DAZFBR010000396          865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFBR010000396 DAZFBR010000000
VERSION     DAZFBR010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.401"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..157
                     /locus_tag="ACHJZ2_005395"
     CDS             <1..157
                     /locus_tag="ACHJZ2_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0019836 - hemolysis by symbiont of host
                     erythrocytes [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Shiga-like toxin beta subunit"
                     /protein_id="HFO4571258.1"
                     /translation="TVKVAGKEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFA
                     EVQFNND"
     gene            complement(454..777)
                     /locus_tag="ACHJZ2_005396"
     CDS             complement(454..777)
                     /locus_tag="ACHJZ2_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO4571259.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
BASE COUNT          226 a          170 c          213 g          256 t
ORIGIN      
        1 cacagtaaaa gtggccggaa aagagtactg gaccagtcgc tggaatctgc aaccgttact
       61 gcaaagtgct cagttgacag gaatgactgt cacaatcaaa tccagtacct gtgaatcagg
      121 ctccggattt gctgaagtgc agtttaataa tgactgaggc ataacctgat tcgtggtatg
      181 tgggtaacaa gtgtaatctg tgtcacaatt cagtcagttg acagttgcct gtcagactga
      241 gcattcgtta aaaaaattcg catggtgaat ccccctgtgc ggaggggcga ctggtgtata
      301 aggtgtcatc tctgacgaca acctggagcg agatacgcgg gttcagggac accggactga
      361 actcaccggg aggcacccgg caccatgtac atgatgatac agatacgcag tgtcagcccc
      421 tctccggagg ggctttttat ctgaatgatt ctgttatttt cgcccgtatg ggcatatttc
      481 cagaatgcag cgatgattaa aacacttatc tgtgatattt cctgtgtttg cagggcactc
      541 ctggctgttt ttaattaaat tccagacgtt tttattaaat ggggccacgt tgtaaatggt
      601 gacggggagc tctttattat tgagtattac gcctgtccgg gttggtgtaa aaatacagcc
      661 cggagggagg aaggtatctg actgatacca taatattaat tttatgttac accatatggc
      721 tgaaaatgat atgccacatg ctggctggat taccgtgtca atcactatcc agttcatttg
      781 ctttccttat tttcttaagg ttttaaatga tgccataaat tataggtgat gttttgaaag
      841 caatctgtca ggctgagttg ttttt
//
LOCUS       DAZFBR010000397          846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFBR010000397 DAZFBR010000000
VERSION     DAZFBR010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.402"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..509)
                     /locus_tag="ACHJZ2_005397"
     CDS             complement(<1..509)
                     /locus_tag="ACHJZ2_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4571260.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQA"
     gene            complement(563..>846)
                     /locus_tag="ACHJZ2_005398"
     CDS             complement(563..>846)
                     /locus_tag="ACHJZ2_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO4571261.1"
                     /translation="AKEANSLVWLWDYANRSQALFRELYPALKQIQSNYSGRCYDYGH
                     EFSYVIGMARDVLINHTRDVDINEPDGPTNLSAWMRLKNKELPPSPHHY"
BASE COUNT          194 a          242 c          209 g          201 t
ORIGIN      
        1 gcctgtgcag aggacagggg cacccggtaa aagccggtga ccgccatatt gtccaggtaa
       61 aggttctgac cgttgcgcca ccagtcatcc tcacgctcaa aatccggcat atcaattccc
      121 gccagatggt aggcatcccg gaacagcgtg taacagtccg tcaccccgtg ctcaaagcgc
      181 cgtcctgtca gatgtggcac acagcggaat ttatgaattt ccccccggca gaccagccac
      241 caggacagtg cactttttat ctgcagccgc cggtcggcct cgctcagcca gggcagacca
      301 ccggggtggc tgtggaccag tgccacaatc tccccctgca tttctgcccg cagccagtcc
      361 tccggcgaca tccggaaata atcttccggc tcaccggaga tattcacgca gggaaaatat
      421 ctttcccctt ccggcgttct caccacgaag ccgcacgact ccgctggtgc acatcgccgg
      481 gcgtgcgcca atatattgct atagagcatg agaactcctg ataaaaaccc agccgaagct
      541 gggtcatttc gttggcaatc tgttagtagt gatgcggtga aggaggtaat tctttattct
      601 taagtctcat ccatgcggaa agattcgttg gtccgtctgg ctcattgata tcaacatctc
      661 gtgtgtgatt aattaaaacg tctctcgcca ttccaataac atacgagaac tcatgaccgt
      721 agtcgtagca tctgccggaa tagttcgatt gaatttgttt tagcgccgga tacagttcgc
      781 ggaataatgc ctgtgagcgg ttggcataat cccatagcca tacaaggctg tttgcttctt
      841 ttgcgg
//
LOCUS       DAZFBR010000398          839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFBR010000398 DAZFBR010000000
VERSION     DAZFBR010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.403"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..376)
                     /locus_tag="ACHJZ2_005399"
     CDS             complement(<1..376)
                     /locus_tag="ACHJZ2_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4571262.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHD"
     gene            353..495
                     /locus_tag="ACHJZ2_005400"
                     /pseudo
     CDS             353..495
                     /locus_tag="ACHJZ2_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(690..803)
                     /locus_tag="ACHJZ2_005401"
     CDS             complement(690..803)
                     /locus_tag="ACHJZ2_005401"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO4571263.1"
                     /translation="MVDFTSRIMSVLADGVLVCGIMVLLWPVIKRNSLHNA"
BASE COUNT          224 a          236 c          204 g          175 t
ORIGIN      
        1 catcatgcag acagtggtca gccggaataa tatcgttata tctgcaggca gccccgcccg
       61 gcgtcactgt actgcggcgc gccagctgtt taatgcgcgg atccggagca tcgtatgaat
      121 ccggcagcgg aagcccttca ccgtaagcca tggcattgga ctgcccggcc agtacgatga
      181 cgtagtacca atccggctca gatgaagggc cgacctgtgg ctctccttca atagccaccg
      241 cctgcatcag tgtgtacggc gtaatggcaa ccggtccgcc gtatggctgc cagccctctt
      301 tcagtttgtg tgtcagcttt tccgcaaggt ctgacggcga cgccgccctg acaacatcat
      361 aatgtttaat cgacatcgaa tttctcccgt gtagaggaac agagttaaaa agccggaagc
      421 ggaatcaaat cacaggatga ccatctgcca gtggctggtc gtaaaaaaag gccgcgccat
      481 gcgcagccga aaataaaggg ataacgatga tagtttgaga aaaacagaaa taacactttt
      541 gtggcaaagc atggtgccgg gtgcctcccg gtgaattcag tatcagcacc tgaatccgcg
      601 attaccccat attccttctt gctgattgcc ccaccgcaca gggggattca ccatgcagaa
      661 gtgtttttaa taaacagcaa acaaaaaaat caagcattat gcaggctgtt tctttttatc
      721 accggccaca gcaataccat aatgccgcag accagcaccc catccgccag caccgacatg
      781 attctgctgg tgaaatccac catcaccacc agaaacagca ggagtgcagc cacagtcag
//
LOCUS       DAZFBR010000399          839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFBR010000399 DAZFBR010000000
VERSION     DAZFBR010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.404"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>839
                     /locus_tag="ACHJZ2_005402"
     CDS             <1..>839
                     /locus_tag="ACHJZ2_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4571264.1"
                     /translation="GILRDEYAAMPETVSSSITKVENAFMAWVGGANEASGVTKTLSG
                     MLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAEAQFR
                     GTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARSAAQNALN
                     STTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDEIAHK
                     TPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTGDG
                     FMI"
BASE COUNT          195 a          205 c          290 g          149 t
ORIGIN      
        1 gggatattac gtgatgaata tgcggccatg ccggaaacgg tttccagtag tatcacaaag
       61 gtggaaaacg cctttatggc ctgggtgggc ggtgcgaatg aggccagcgg ggtgacaaaa
      121 acgctctccg gcatgctgaa cggtgttgcc ggacagattg ataatgtggc aacagccgtg
      181 ggcgcgctgg ttgccgtcgg ggttgcccgg tactttggca atatggcctc cggagcgatg
      241 tctgccacgg caggacttgt gacggctgca cgtaatgaag ttgcactggc ggaagcacag
      301 ttcaggggaa cgcagattgc cacggcgcgg gcaagggcag ccgtgtaccg tgctcagcag
      361 gccgtggcgg cagcccgcgg gacggagatg cagattgctg cagaggcccg tctggcggcc
      421 acacaggaac gcctgaacag aaatattgct gccagaagcg ccgcccagaa tgcgctgaac
      481 agtacaacgg cggtgggctc acgtctgatg agcggtgcgc tggggctggt tggtggcgta
      541 cccggactgg tgatgctggg ggctgcagca tggtacacgc tgtaccagaa tcaggagcag
      601 gccagggagt ctgcgcgcca gtatgcactg acgatagatg aaatcgcgca taaaacgccg
      661 tcaatgtctt tgcctgaagc ctcagataat gaaggacgaa cacgggcggc gctgacagag
      721 cagaaccggc tgattgatga acaggccagt cgggtgaaat ccctgcagga aaaaatcgca
      781 ggatatcagt atgttctggc gaacccgggc tggacgaccg gtgacggatt catgataaa
//
LOCUS       DAZFBR010000400          838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFBR010000400 DAZFBR010000000
VERSION     DAZFBR010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.405"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..569
                     /locus_tag="ACHJZ2_005403"
     CDS             <1..569
                     /locus_tag="ACHJZ2_005403"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4571265.1"
                     /translation="YFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYL
                     KLQALFARVAEEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVP
                     YTDVWTHKPVQYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALG
                     RRATGVELETERFEQTVREVQDLVSQNG"
     gene            complement(742..819)
                     /locus_tag="ACHJZ2_005404"
                     /pseudo
     CDS             complement(742..819)
                     /locus_tag="ACHJZ2_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          214 a          188 c          257 g          179 t
ORIGIN      
        1 cttactttcg tgatgcgcgt gctgccctgg ggataacggc aaaacagata gtggatgcca
       61 caggaaagaa aaacatggtg tcgcactggt tcagtgccag tcagtggcag ctaccgaacg
      121 aaagcgatta tctgaaatta caggcgctgt ttgcccgggt ggcagaagag aagcatcagc
      181 ggggtgaact ggagaagccc caccaccagc tgctggagac gtatacttca ctgaaccggc
      241 agtatgcgga actacagagt gaatataagc atctgcggcg gtattttggc gtgacggcgc
      301 aggtgccgta cacggatgtg tggacgcata aaccggtgca gtactatccc gggaaacatc
      361 cgtgcgaaaa accggcagaa atgctgcagc agataatcag tgcgagcagt cgtccgggtg
      421 acctggttgc agatttcttc atggggtcgg gttcgacagt caaagccgcg atggcgctgg
      481 ggcgtcgtgc aactggcgtt gagctggaga ctgaacgttt tgagcagacg gtcagggaag
      541 tacaggattt agtcagtcag aacggatgat attgcagaat taaccgggta tcaggtacgc
      601 cccgaaaatt ttaaatgtct cacaattcag acggttgaca gttgtctggt ttgcggggag
      661 tttgttaaaa gaaactggca tggtgaatcc ccctgagcgg aggggcatat cagcgcaggt
      721 gtttctactc tatcctttct gtgcgggttc aggtgctgat actgaattca ccgggaggca
      781 cccggcacca tgcaagaaaa agaatgtgca tgcaaacatg cccctctccg gaggggca
//
LOCUS       DAZFBR010000401          833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFBR010000401 DAZFBR010000000
VERSION     DAZFBR010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.406"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            9..104
                     /locus_tag="ACHJZ2_005405"
     CDS             9..104
                     /locus_tag="ACHJZ2_005405"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO4571266.1"
                     /translation="MEGKQPKNSGGNFTMLIAKKDGQVLNVIHTK"
     gene            739..>833
                     /locus_tag="ACHJZ2_005406"
     CDS             739..>833
                     /locus_tag="ACHJZ2_005406"
                     /inference="COORDINATES: protein motif:HMM:NF040040.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6258 family protein"
                     /protein_id="HFO4571267.1"
                     /translation="MSRIAYGTTEWNFYDNEDLEDGYLVFSGVSF"
BASE COUNT          274 a          138 c          180 g          241 t
ORIGIN      
        1 gttggaaaat cgagggaaaa caaccgaaga attcaggagg aaattttaca atgctaatag
       61 cgaaaaaaga tgggcaagtg ttgaatgtaa tacacacaaa ataatccact gtacagtcga
      121 ttcaaatatt ttaccaaggt gtgtttcgta acaattttgt gtacaatatg actctacata
      181 tcgacgaaaa aaccacttga tttgacagga atacattcaa aaaatttctt ttttcatttc
      241 aggagcgatt ttcatctgtt acttgaagta tctgtgagat taagtgactc cccctaacgt
      301 cgtactaaat agtaattact ggccataccg ggagcagtat tcggcgctcc ccacatgcag
      361 cagaataacc gcatcagcga agatgtattt ttactatcac aatgtgtacg gacggtttat
      421 ggaaaagaat gaacattaga tacgcgatgc ggcggcaggt tacacggtta tcactacgat
      481 agccaatact ggctggtgca ttaccgccgt gaacaatagg acgccgtact gctggaaatt
      541 tcttaccttt ataactcccc gcgccgccgt atcgggaaac gggaagcaat caagatgagg
      601 ctgatataat gtgcggaatg cctgagtatt tttagaatag gggggtaata tgtttgatag
      661 aatatatctt ggtgatcgag caataaaaaa attgaatttg atttatggag taaacaaatt
      721 agaattcagg ttaatttaat ctcaaggata gcctatggta caacagaatg gaatttttat
      781 gataatgaag acttagaaga tggttatttg gtattttcag gtgttagttt ctt
//
LOCUS       DAZFBR010000402          829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFBR010000402 DAZFBR010000000
VERSION     DAZFBR010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.407"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..466)
                     /locus_tag="ACHJZ2_005407"
     CDS             complement(<1..466)
                     /locus_tag="ACHJZ2_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4571268.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLVGE"
     gene            complement(580..828)
                     /locus_tag="ACHJZ2_005408"
     CDS             complement(580..828)
                     /locus_tag="ACHJZ2_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4571269.1"
                     /translation="MQRLCGCGKHDDREHGELLTAQLRLGPADILESDENGIIPEQDR
                     VITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          240 a          236 c          145 g          208 t
ORIGIN      
        1 cttcaccaac caaacgagaa aatgcagcgg catcaaataa agtacataca tctgaaccat
       61 ctgaattctt cacaaaaata cctttttcag gctgctccag tgtaattgga cactgaatag
      121 cctcaagcgg gcactgaagt ctctctggac aaacagaaaa cttacattga gatattttgt
      181 tctgaatact ctcttgcgat tgtgcttcac ctgagtccga aagcatactt agcattctct
      241 caccaagggc tcgaggacca ctattaaatc ccaaaagcaa taactcagaa agcaatccgc
      301 cattaccgcc actggatgaa aagcgcccat tagtagaatc atacacaata ctgacagttt
      361 caccaccgac tgtaatatct ccgcgcccat tggctacaat tcgggcacga acaacctcca
      421 ccccaagatt aaatgaatgt gatctaatat ctgaggttaa tggcataaca aagctcccta
      481 ttttaatttg aactccagac ttaaatagct gtaacaaaca tcccccatac gtgacaacaa
      541 aaaaccggag ccggactccg gtttttgtga agctgtcggc tatttcatcc cgccaatatt
      601 ttcccacgtc ccgtcagcac gcaggatttg cagcggtctt accacacact gtatctgctt
      661 tttatccgca tccagtatca ccacctgcgt gattaccctg tcctgctccg ggataatacc
      721 attctcatct gactccagga tgtctgccgg ccccagtcgc agctgtgctg taagtaactc
      781 cccgtgttca cggtcatcat gctttccgca accacacaga cgctgcata
//
LOCUS       DAZFBR010000403          826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFBR010000403 DAZFBR010000000
VERSION     DAZFBR010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.408"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>826
                     /locus_tag="ACHJZ2_005409"
     CDS             <1..>826
                     /locus_tag="ACHJZ2_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4571270.1"
                     /translation="LHIAKKLLPYIPNNAGILLVPCCRGGSAFTQGAEGTFSADAGAS
                     QDSARWGVGKPLYQDLIARTKAALQKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTA
                     MLAQFRADLSVFNAQCHGGSAADVPWICGDTTYYWKNTYGTQYNTIYGAYKNRESEGV
                     YFVPFMTDGNGVNTATNAPAEDPDIPASGYYGAASRTNGNQVSSNRPTHFSSWARRSI
                     IPDRMATAILNAAGRTSAFISGKAPEIKPSPGGNTPSGPSADTSVRTISLLPAAGEA"
BASE COUNT          192 a          226 c          240 g          168 t
ORIGIN      
        1 ttacatattg ccaaaaaact gcttccgtat atcccgaata acgcggggat cctgctggta
       61 ccatgctgtc gtggtggttc ggcattcacc cagggcgcgg aggggacatt cagtgcggac
      121 gcgggggcca gccaggattc ggcgcgctgg ggtgtgggta aaccgttata tcaggacctg
      181 attgcgcgca ctaaagctgc attacagaag aacccgaaaa atgtgttgct ggcggtgtgc
      241 tggatgcagg gagagtttga catgagcgcc gccacccacg cacagcaacc tgcgctgttt
      301 acagccatgc tggcacagtt tcgtgctgac ctctccgtgt ttaacgcgca gtgccatggt
      361 ggcagtgctg cagatgtgcc gtggatttgt ggtgacacga cgtattactg gaaaaatacc
      421 tacggcaccc agtacaacac catttacggg gcgtacaaaa acagggagag tgagggcgtt
      481 tattttgtgc ccttcatgac agacggtaac ggcgtcaata ccgccactaa cgcgccggca
      541 gaagatccgg atattccggc atcaggatat tacggtgcgg catcgagaac gaatggaaac
      601 caggtatcat caaaccgccc gacacatttc agttcatggg cgcgcaggag cattattccg
      661 gatcgtatgg caaccgctat tctgaacgca gccgggcgca cctcagcctt catcagtggt
      721 aaggcaccgg aaatcaaacc ctcgcccggc ggcaacacgc catcgggtcc gtctgcagat
      781 acgtccgttc gcacaatctc cctgctgccg gcagccggag aggctg
//
LOCUS       DAZFBR010000404          814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFBR010000404 DAZFBR010000000
VERSION     DAZFBR010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.409"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..367
                     /locus_tag="ACHJZ2_005410"
     CDS             <1..367
                     /locus_tag="ACHJZ2_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571271.1"
                     /translation="KIVDVINTWSIDEKLTWNALCDRLVRVIGKRPSRQSLSSHVRIA
                     ESFNVKKTTIKSGVIHTVKPANLKVASQRIKRLEAENEALKALNERYLEQFKVWLYNA
                     HLKQITIEELNNPLPAKYL"
     gene            465..>814
                     /locus_tag="ACHJZ2_005411"
     CDS             465..>814
                     /locus_tag="ACHJZ2_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571272.1"
                     /translation="MDKTNINLMERYLLLLDRFVDKLAESGFSEQRIIEQSYLFCAGF
                     YIKYQSGIEKMTFSNREVVLTFLLLSYYSHINKLDDDLINKERMKHVCSSLINFIVSN
                     GSRTEKVYANEKRK"
BASE COUNT          294 a          119 c          150 g          251 t
ORIGIN      
        1 gaagattgta gatgttatta acacatggtc tatagatgaa aaactaacct ggaatgcttt
       61 gtgcgatagg ttagtcaggg ttattgggaa aagaccatca agacaatcct taagtagtca
      121 tgtaaggatt gcggaaagct tcaatgtgaa aaaaaccaca atcaaatctg gtgtaataca
      181 cactgtaaaa cctgctaatt taaaagtggc ttctcaacga attaaaagac ttgaagcaga
      241 aaatgaagct ttgaaggcat taaatgaacg ctatttagag cagttcaaag tatggcttta
      301 taatgctcac cttaaacaaa taacgattga agaacttaat aatccgttac ctgcaaaata
      361 cctttaataa aattgatagg ttttagctat tgacatggtg aaatattatg ttatttattt
      421 tcttgtcagg acgacaaaat ttcactattg caaggatgca tattatggac aaaacaaata
      481 ttaacttaat ggaaaggtat cttcttctgc ttgatcgatt cgttgataaa ctcgctgaat
      541 caggtttctc agagcaacga attatcgaac aatcatatct tttttgtgca ggattttata
      601 tcaaatacca gagtggtata gaaaaaatga ccttctccaa cagggaggtt gttttaacct
      661 ttctgctact ttcatattat agtcatataa ataaactgga tgatgatttg atcaacaaag
      721 agcgtatgaa gcatgtatgt tcttcattga ttaactttat agtcagtaat ggtagccgaa
      781 cagagaaagt ctatgctaat gagaaaagga aata
//
LOCUS       DAZFBR010000405          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFBR010000405 DAZFBR010000000
VERSION     DAZFBR010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.410"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            127..333
                     /gene="ybcW"
                     /locus_tag="ACHJZ2_005412"
     CDS             127..333
                     /gene="ybcW"
                     /locus_tag="ACHJZ2_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFO4571273.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(498..692)
                     /locus_tag="ACHJZ2_005413"
     CDS             complement(498..692)
                     /locus_tag="ACHJZ2_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4571274.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
BASE COUNT          221 a          149 c          180 g          241 t
ORIGIN      
        1 ctggcattct ggagggaaat acaaccgaca gatgtatgta aggccaacgt gctcaaatct
       61 tcatacagaa agatttgaag taatatttta accgctagat gaagagcaag cgcatggagc
      121 gacaaaatga ataaagaaca atctgctgat gatccctccg tggatctgat tcgtgtaaaa
      181 aatatgctta atagcaccat ttctatgagt taccctgatg ttgtaattgc atgtatagaa
      241 cataaggtgt ctctggaagc attcagggca attgaggcag cgttggtgaa gcacgataat
      301 aatatgaagg attattccct ggtggttgac tgatcaccat aactgctaat cattcaaact
      361 acttaacctg tgacagagcc aacacgcagt ctgtcactgt caggaaagtg gtaaaactgc
      421 aactcaatta ctgcaatgcc ctcgtaatta agtgaattta caatatcgtc ctgttcggag
      481 ggaagaacgc gggatgttca ttcttcatca cttttaattg atgtatatgc tctcttttct
      541 gacgttagcc tccgacggca ggcttcaatg acccaggctg agaaatttcc ggaccctttt
      601 tgatcaagag cgatgttaat ttgttcaatc atttggttag gaaagcggat gttgcgggtt
      661 gttgttctgc gggttctgtt cttcgttgac atgaggttgc cccgtattca gtgtcgctga
      721 tttgtattgt ctgaagttgt ttttacgtta agttgatgcg gatcaattaa tacgatacct
      781 gcgtcataat t
//
LOCUS       DAZFBR010000406          781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFBR010000406 DAZFBR010000000
VERSION     DAZFBR010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.411"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..186
                     /locus_tag="ACHJZ2_005414"
     CDS             <1..186
                     /locus_tag="ACHJZ2_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001509878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571275.1"
                     /translation="KKIDKKIEEIDNAIRDLSSETERFKIKYQQENTLNDVVQNIRTW
                     LKENQNMVKAIKKIDTE"
BASE COUNT          280 a          130 c          142 g          229 t
ORIGIN      
        1 aagaaaatag ataaaaaaat tgaagaaata gataatgcaa tccgggatct ttcctctgaa
       61 acagagaggt tcaagataaa gtatcaacaa gagaatacac ttaatgatgt agttcaaaat
      121 atcaggactt ggctaaaaga aaatcaaaat atggtgaaag ctattaagaa aattgatact
      181 gaataacatg gcgaataaat aaacggatca agtcgttcct atgacaatga ggttatacga
      241 aaagacgctt actcatcaga aatctggtat gctttaatta aaccagtgtc gctgatgtgt
      301 aagcgttgac gttgggagac gattaaatgg ttataccact cctacgtctt ttccatacgg
      361 cgtataagag tgcaacatgt tgagttttcc gagtgaatag tgcattttac taaccgagcc
      421 atttgaatca accaacgcaa taaaaaagac tggaataata agcctgtatt tattatttat
      481 cgcaatcgca ctttacggtt ttatccaact gaaaagagac tgcttccagc ggttgtggat
      541 tatccctggg cgatagtcat agtctggtgt caggattcac aaatgacctg tttccttaca
      601 tggatataaa cacaacatag agtgcggtag ttaatctgaa taatctaatt tcggctattc
      661 agcacaaaac aacattattg attctttata agagagaatc tctccatcat ttttatataa
      721 atcattatta agaaaatcca gaagctcttg ccttacacta taaatattga ctttttggtc
      781 a
//
LOCUS       DAZFBR010000407          780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFBR010000407 DAZFBR010000000
VERSION     DAZFBR010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.412"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            157..726
                     /locus_tag="ACHJZ2_005415"
     CDS             157..726
                     /locus_tag="ACHJZ2_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO4571276.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
BASE COUNT          220 a          164 c          202 g          194 t
ORIGIN      
        1 ccgtaatgat gcctttgtca tttctgcgca tctcacgcgc atctcacaac acagaacctt
       61 tcaggatgac ccttgaggat accggtttgg ctgtcggtgc ctttctgtgg gctggattcc
      121 tgtgagacaa ggttcatcac taaaaggaaa taaccgatga atatgatggc cgtgccgttt
      181 cacggcaact ctctttatgt agttaaccat aatggcgaac catacgttcc catgaaacct
      241 gtcgttgcgg ggatggggct ggcctggcaa tcacagttgg ctaagttaag acagcgtttt
      301 gcgtcaacta taacggaaat cgttatggtt gctgaggatg ggaaacaacg caatatggtg
      361 tccatgccac ttcgaaaact tgccggctgg ctacaaacca ttaatcccaa caaagtaaaa
      421 cccgaaatcc gcgataaggt catccggtat caggaagagt gcgacgatgt tctttacgag
      481 tactggacga agggttttgt cgttaatccc cgtaaaatga gcgtgatgga agaactcaac
      541 caggcttgtg ctgacatgaa acgggataaa aacattgcca gtgtgtttgc taccgggctg
      601 aatgagtgga aacaggttaa agccgcgcat gtatcaaaaa tccgtacgct ggtaaatgaa
      661 gcgaatatgc tgattgattt tgtcctggct gatacaggca aagggaaaat aacaaaggcg
      721 gattgatggg gtggctaatg atatcagata aactcataac gctggtgaag agcctctgtg
//
LOCUS       DAZFBR010000408          779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFBR010000408 DAZFBR010000000
VERSION     DAZFBR010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.413"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            126..>779
                     /locus_tag="ACHJZ2_005416"
     CDS             126..>779
                     /locus_tag="ACHJZ2_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO4571277.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQITTDTDTDKE
                     LNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA
                     "
BASE COUNT          210 a          177 c          228 g          164 t
ORIGIN      
        1 tggggcaccc tgactaacag tggtggtcag cctggtaatg ctgttgtcgt gcattgacca
       61 acaatattca tgccggattt cttccggatg ttcgagggta aagttcggta tcagatgagg
      121 tgagtatggc taatgcctgg ctcagattgt ggcatgacat gccaaatgat cccaaatggc
      181 gaaccattgc cagggtctca ggacagccaa tcgcaacagt gatggcggtg tatatccacc
      241 ttctggtgag tgcgtcacga aatgtcacga catgtcacgg cgtgtcacta cgtggtcaca
      301 ttgatgtcac gacggaagat ttagcaagtg cgcttgatgt gacggaagac gtgattgatt
      361 caattttgca tgcaatgcag gggcgagttc tggatggtga tcttatttcc ggatgggaaa
      421 aacgccaggt gatgaaggag gataacggta atgtttcgca aaccgcaaaa tccccggcag
      481 agcgcaagag agcgcagcgg gagcgggaaa gactgcggaa gcagaacact gattgtcacg
      541 atgagtcacg acgcgtcacg catatgtcac gacaaatcac gacagataca gatacagata
      601 aagaattaaa ccccacacat aacgcgcgca tgcgcgagag tgctacgacc ggtgagttga
      661 atgacgcgtc gttgcagaca gccgaacctg aatacctgga cggcctgagc gaaccgatcg
      721 ggaaatttcc gatgactgga gtctggcaac cgtcgccgga ttttcgacag cgggcagca
//
LOCUS       DAZFBR010000409          774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFBR010000409 DAZFBR010000000
VERSION     DAZFBR010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.414"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..175)
                     /locus_tag="ACHJZ2_005417"
     CDS             complement(<1..175)
                     /locus_tag="ACHJZ2_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO4571278.1"
                     /translation="MKACLLLFFYFSFICQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSE"
     gene            complement(187..>774)
                     /locus_tag="ACHJZ2_005418"
     CDS             complement(187..>774)
                     /locus_tag="ACHJZ2_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4571279.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDL
                     IHSVADEINIPGVFTIGGHGTPTSIESAKRSIMTAKDLAYLIKFDGNYKDGMTVWLFS
                     CNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDL
                     MAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          219 a          172 c          123 g          260 t
ORIGIN      
        1 cctctgaatc accatgttgt gatttatact tcagtttcat caacttttct tcacttaaat
       61 cataggtcat tgctgtagac cccatcatac tttcgttttg ttttattttc acatcagcac
      121 catgcaattg acaaataaaa gagaaataaa aaaatagtaa caagcatgct ttcatacatc
      181 tacaacctat cttatatcac tgggtttttc tggcgtacct tgcatattag aaatggggtg
      241 cccagatggc ccatatgtta tccaattacc aaggtctttt gttgttatag ccattagatc
      301 cttatttgaa ttaaggttcg ttggtgctgt tagcactgta tccattttta acttaccatt
      361 agttcctcgc ccccaccacg tccatagcgt gtcaggtcct attacatttg tatgtaactc
      421 tttagctaat tggctagcaa atgaattttg tcctttacct gtattacaag aaaataacca
      481 aactgtcatc ccatctttat aattcccatc aaatttaatt agatatgcta gatctttagc
      541 tgtcatgata ctgcgctttg cggattcaat agatgtgggg gtaccatgcc ccccgattgt
      601 gaaaacgcct gggatattaa tctcatcagc tacagaatgg ataagatcac tttcggggta
      661 taaatttata tcaaccaaac cttgcggatc tacatttatg accggattca acggatactg
      721 ataaaaattc catcccccct tcagtccaat cggatcctga gtgatatatc gccc
//
LOCUS       DAZFBR010000410          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFBR010000410 DAZFBR010000000
VERSION     DAZFBR010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.415"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>767
                     /locus_tag="ACHJZ2_005419"
     CDS             <1..>767
                     /locus_tag="ACHJZ2_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HFO4571280.1"
                     /translation="TDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRY
                     LYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQP
                     GSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILA
                     DRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIHLYHCDHRGLPLALISKEGATEW
                     CAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLY"
BASE COUNT          175 a          219 c          252 g          121 t
ORIGIN      
        1 aaccgacctc atcccggaag gggttatccg cacggatgat gagcggactc accggtacca
       61 ttacgacagt cagcaccggc tggtgcacta cacgcggaca caatatgaag agccgctggt
      121 cgaaagccgc tatctttacg acccgctggg ccgcagggtg gcaaaacggg tgtggcgacg
      181 tgaacgggac ctgacgggct ggatgtcgct gtcacggaaa ccgcaagtga cctggtacgg
      241 ctgggacggc gaccggctga ccacaataca gaacgacaga acccgcatcc agacgattta
      301 tcagccgggg agcttcacgc cactcatcag ggttgaaacc gccaccggtg agctggcgaa
      361 aacgcagcgc cgcagcctgg cggatgcgct tcagcagtcc ggcggcgaag acggtggcag
      421 tgtggtgttc ccgccggtgc tggtgcagat gctcgaccgg ctggaaagtg aaatcctggc
      481 tgaccgggtg agtgaggaaa gccgccgctg gctggcatcg tgcggcctga ctgtggcgca
      541 gatgcaaagc cagatggacc cggtatacac gccggcgcga aaaatccacc tgtaccactg
      601 cgaccatcgc ggcctgccgc tggcccttat cagtaaggaa ggggcaacag aatggtgcgc
      661 agaatacgat gagtggggca acctgctgaa tgaagagaac ccgcatcagc tgcagcagct
      721 tatccgcctg ccggggcagc agtatgatga ggagtccggc ctgtatt
//
LOCUS       DAZFBR010000411          761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFBR010000411 DAZFBR010000000
VERSION     DAZFBR010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.416"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
BASE COUNT          259 a          157 c          140 g          205 t
ORIGIN      
        1 caaataaaac agaataaaaa ccaagctgaa ccgtttcact atactctact taattcgagt
       61 tacaggaagg cggcaagcga gtgaatcccc aggagcttac ataagtaagt gattggggta
      121 agcgaacgca gtcgcagcac atgcaacttg aagtatgacg agtatatata tctattagct
      181 gtttcccgga cataagctac agccagcact aacctggaac gggtgcatta taaaaaggaa
      241 caagggacaa atagctgaac agtaaccgcg ttagtctctt cccgcataac cgtaccattc
      301 gtattaagaa gtattgccca tatccattcg gcgaaagagg atctgccata catcaagtcc
      361 gttttacttg gcatcagatc atcgccgtca ttaactctgt taaagtcgga caaacgatta
      421 gcaagatctg tgattcaaaa gtgcttagcg ctaaggtggg actggttaca gattcattaa
      481 ccctgttctt tgtcgcacct atcgatcggt gtaatgccac aacaggttta tgttaatcag
      541 aacaatgcgc ccctctataa agagaattct ttgataaggc aacttgaaaa gggaaagcta
      601 actgatatca atgtaccact ttagtaatca tcacattata tacaggccaa aataagtttc
      661 ctgaaagcca gtatctctaa atcaatcatt aatgatatat tttatcgtca caataaaaaa
      721 ttatctaaat tctattactt atgtaaataa gtacaacctt t
//
LOCUS       DAZFBR010000412          756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFBR010000412 DAZFBR010000000
VERSION     DAZFBR010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.417"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            27..743
                     /locus_tag="ACHJZ2_005420"
     CDS             27..743
                     /locus_tag="ACHJZ2_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4571281.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGISEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          251 a          131 c          163 g          211 t
ORIGIN      
        1 aataaatttt cgaggagatc attcctgtgg gacgtaaatg ggccaatatt gttgctaaaa
       61 aaacggctaa agacggtgca acgtctaaaa tttatgcaaa attcggtgta gaaatctatg
      121 ctgccgctaa acaaggtgaa cccgatccag aattaaacac atctttaaaa ttcgttattg
      181 aacgtgcaaa gcaggcacaa gttccaaagc acgttattga taaagcaatt gataaagcca
      241 aaggcggcgg agatgaaaca ttcgtgcagg gccgttatga aggctttggt cctaatggct
      301 caatgattat cgccgagaca ttgacttcaa atgttaaccg tacgattgct aacgttcgca
      361 caattttcaa taaaaaaggc ggcaatatcg gagcggcagg ttctgtcagc tatatgtttg
      421 acaatactgg tgtgattgta tttaaaggat cagaccctga ccatattttt gaaattttac
      481 ttgaagctga agttgatgtt cgtgatgtga ctgaagaaga aggtaacatt gttatttata
      541 ccgaacctac tgaccttcat aaaggaatcg cggctctaaa agcagctgga atcagtgagt
      601 tctcaacaac agaattagaa atgattgctc aatctgaagt tgagctttca ccagaagatt
      661 tagaaatctt tgaagggctt gttgatgccc ttgaagatga cgacgatgta caaaaagttt
      721 atcataacgt cgcaaatctc taattatatt ttaaag
//
LOCUS       DAZFBR010000413          748 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFBR010000413 DAZFBR010000000
VERSION     DAZFBR010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.418"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            2..559
                     /locus_tag="ACHJZ2_005421"
     CDS             2..559
                     /locus_tag="ACHJZ2_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4571282.1"
                     /translation="MRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYARLHEPLGE
                     GAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFS
                     LGASMILGGVAQMLAPKAKTPDYRATDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVG
                     SRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          171 a          174 c          244 g          159 t
ORIGIN      
        1 catccgggcg ctgtcgttac aggtgccggg attccgccgt cagatgaacg aaggctggta
       61 ccagatacgt attcgcggtg aggacacggc accggaggcg gtgtacgccc gtcttcacga
      121 acctctgggt gagggggcgg tcatccatat tgtgccgcga ctggccgggg ccggaaaggg
      181 tggactgcag attgtgctgg gggcggcagc catcgtgggc tctttcttca cggccggggc
      241 atcaatggcg ttatggggtt cagccctggc agccggtggt ttttctgcca ccacgatgct
      301 gttttcactg ggggccagca tgatactggg cggtgtggcc cagatgctgg caccgaaggc
      361 aaaaacaccg gattaccgcg caacggataa cggtaaacag aacacgtatt tttcgtcact
      421 ggataacatg attgcccagg ggaacccgat gccggtgcct tatggtgaaa tgctggttgg
      481 ttcacgacgg atatcccagg acatcagcac ccgtgatgag ggcggagacg ggaaagtggt
      541 ggttatcggg cggggatgaa aataaaaaaa tcccgcagag ttagcggagc tgcgggagag
      601 aacgatgaag attaacgtta tggagttatt tttcaggcat caaaaaagta atgcagcgtc
      661 attattgcgg ctacaggcaa ttgccggaaa tgtgaagagt ttcagaaatt ttattccgtc
      721 atgacacagg caccctccgg ggtgcctg
//
LOCUS       DAZFBR010000414          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFBR010000414 DAZFBR010000000
VERSION     DAZFBR010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.419"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..275)
                     /locus_tag="ACHJZ2_005422"
     CDS             complement(<1..275)
                     /locus_tag="ACHJZ2_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4571283.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTIS"
     gene            337..732
                     /locus_tag="ACHJZ2_005423"
                     /pseudo
     CDS             337..732
                     /locus_tag="ACHJZ2_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          197 a          185 c          173 g          177 t
ORIGIN      
        1 gagatcgtcg aaggactgcg attcagcgca gtagctatcg cacgaatgct cattttggct
       61 gacaaaccag ctcgtatctc ctcgcgctca gacagtgtca ggtgagctac agcccgctta
      121 cgctcatggg gttttatgcc gccagtatcc cttaacatag tgaagatcgt tccgggtttt
      181 gaacccagga tattcgctat ttcactgaag cctgttccgt tcttccatag ttcaaaaaca
      241 gaggcttttt cctctgctgt aaatgttcgt ctcattcaaa aaacctccgc aaccccatgt
      301 tttcacataa ctgttgcgtt gaccaattga atctacaaca gcgtattttg cacaggagtc
      361 gctgaaaaat acgcgttaat cgaacaatgg cgacaacaat ttcccattga agcgatgtgt
      421 caggtatttg gtgtatccag gagcggttat tacaactggg tacagcatga accctcagac
      481 agaaaacaaa gtgatgagcg gctaaaactg gagattaagg tggcacatat ccgcactcgc
      541 gaaacatatg gaacccggcg gctccagacg gagctggcag agaatggcat catcgttggt
      601 cgtgaccgac tggcacgtct tcgtaaggag ctaaggctac gctgtaagca gaaacgcaag
      661 ttcagggcga ctacgaactc gaaccacaat ctgccagttg cgccaaatct gctgaaccag
      721 acgttcgctc ct
//
LOCUS       DAZFBR010000415          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFBR010000415 DAZFBR010000000
VERSION     DAZFBR010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.420"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..96
                     /locus_tag="ACHJZ2_005424"
     CDS             <1..96
                     /locus_tag="ACHJZ2_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4571284.1"
                     /translation="EMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            439..>731
                     /locus_tag="ACHJZ2_005425"
     CDS             439..>731
                     /locus_tag="ACHJZ2_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571285.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKA"
BASE COUNT          202 a          148 c          228 g          153 t
ORIGIN      
        1 gaaatgctgg ttggctcccg ccgtatatcc caggacatca gcacccgtga tgaaggcggg
       61 ggcggaacgg tcgtggttgt cgggcgacag ggataaaaca taaaaaaatc ccgcagtgat
      121 cgcggagctg cggggacaga caaatgaaga tcaatgttaa ggagttgttt ttgttactcg
      181 ggcaaaaaaa cactaacgca gcgaaattat aagcgccaca gtcagtgtgt gaaaatgtga
      241 agatattcag aatttttatg ccattaccgg ttttaaccaa caggattatc ggtgggcatg
      301 aaagaaaacc ccggtatctg ctgataccgg ggtttctctt tagcatggca gaaatgtgtt
      361 tcatgctttt cgggcgaagg atatccgact tctgtacgga atggcaagtg gcggttaatt
      421 tattcagggg aaggctgtat gggaaaaggt ggcggtaagg cacacacgcc tcgtgaggcg
      481 aaggataatc tcaaatccac gcagatgatg agtgtgattg atgcgattgg tgagggaccg
      541 atagaaggtc cggtgaaggg actgcagagt attctggtga acaaaacccc actgacggac
      601 acggacggca atcccgtgat acacggtgtg accgcggtct ggcgtgccgg ggagcaggag
      661 cagacaccac cggaaggctt tgagtcctcc ggagctgaaa ccggactggg cgtggaagtg
      721 acgaaggcaa a
//
LOCUS       DAZFBR010000416          726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFBR010000416 DAZFBR010000000
VERSION     DAZFBR010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.421"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..191
                     /locus_tag="ACHJZ2_005426"
     CDS             <1..191
                     /locus_tag="ACHJZ2_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571286.1"
                     /translation="NQSTTDAQGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTPTTSI
                     SDLLVVVMKKSTAGISIS"
     gene            258..>726
                     /locus_tag="ACHJZ2_005427"
     CDS             258..>726
                     /locus_tag="ACHJZ2_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4571287.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNT"
BASE COUNT          176 a          176 c          202 g          172 t
ORIGIN      
        1 ttaatcagtc cacgacggat gctcagggcg tattttcctc agttatagat atgcctgccg
       61 gacaggggac gctgacactg acattcaccg tatcttcatc aggagcgaat aactggacac
      121 caacaaccag tatcagcgat ctgctggttg tggtgatgaa aaaatccaca gcaggtatca
      181 gtatcagctg aattttataa cccagaacgg gcgtcagaaa tgacgccttt tttattgcag
      241 aaaagcgaga ggtaattatg cgtaaacttt atgccgccat tttgtccgca gccatttgtc
      301 tggccgtatc cggtgcgcct gcatgggcat ctgaacatca gtccacgctg agcgcggggt
      361 atcttcatgc ccggaccaac gttcccggca gtgatgatct gaacgggatt aacgtgaaat
      421 accgttatga gtttacggat acgctggggc tggtgacgtc attcagctat gcaggagaca
      481 agaatcgcca gctgacccgt tacagcgata cccgctggca tgaagattcc gtgcgtaacc
      541 gctggttcag cgtgatggcg gggccgtctg tgcgcgtgaa tgaatggttc agcgcgtatg
      601 cgatggcggg tatggcttac agccgtgttt cgactttttc cggggattac cttcgcgtaa
      661 ctgacaacaa ggggaaaacg cacgatgtgc tgaccggaag tgatgacggt cgccacagca
      721 acacgt
//
LOCUS       DAZFBR010000417          725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFBR010000417 DAZFBR010000000
VERSION     DAZFBR010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.422"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>725
                     /locus_tag="ACHJZ2_005428"
     CDS             <1..>725
                     /locus_tag="ACHJZ2_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4571288.1"
                     /translation="LAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQETEQNDQKA
                     EQNSPNTQQNEPETKQPEPVAQQEPEKICTACGQTGGGNCPDCGAVMGDATYQEIFDE
                     EYQVEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTSTSR
                     AGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIK
                     WWLKQSREAQSAIMTDEIPLDDAL"
BASE COUNT          231 a          171 c          193 g          130 t
ORIGIN      
        1 ctcgccgcat cacgtggtga atttgttgca gggattagcg acccgaatga tccgaaatgg
       61 gtgaagggga ttgaaacccg cgattctgtg aaccagaacc agcaagaaac ggaacagaac
      121 gaccagaaag cggaacaaaa cagcccaaat acgcaacaaa acgagccaga aacgaaacaa
      181 cctgagccag tagcgcaaca ggaaccggaa aagatctgca ccgcctgcgg tcagaccggc
      241 ggcggcaact gccctgattg tggcgcggtg atgggcgacg caacatacca ggaaatattc
      301 gatgaagagt atcaggttga agttcaggaa aatgatccgg aggaaatgga aggtactgca
      361 catcagcaca aggagaacac tggcggcaat cagcatcatg ccagcgatag tgaaactggc
      421 gaggcgtcag atcccttaat taaggcgaac ggtcatcata atctcacatc caccagcaga
      481 gcggggattc atctgatgat cgaccttgaa accatgggaa aaaatcctga tgccccgatc
      541 atctcaatag gtgcaatatt tttcgatccg caaaccggag atatgggacc ggaatttagt
      601 aagactatcg atctggaaac tgctggcggg gtcattgatc gggacaccat taaatggtgg
      661 cttaagcaat cacgcgaagc gcaatctgcc attatgaccg atgaaatccc gttagatgat
      721 gcact
//
LOCUS       DAZFBR010000418          724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFBR010000418 DAZFBR010000000
VERSION     DAZFBR010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.423"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>724
                     /locus_tag="ACHJZ2_005429"
     CDS             <1..>724
                     /locus_tag="ACHJZ2_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4571289.1"
                     /translation="AQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGG
                     QISDSFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVA
                     RVAAGGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAG
                     GGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVK
                     ALASLNQQYNFLSVSQLRHIDEL"
BASE COUNT          159 a          183 c          241 g          141 t
ORIGIN      
        1 gcacaaaaag ccgggatatc agtcggacag tataaggctg ccatgcgcac actgcccgca
       61 cagttcacgg atattgtcac tcagcttgcc ggtggtcaga atcccttcct tatcatgctg
      121 cagcaggggg ggcagatcag cgattcattc ggtggaccgc tcagcctgct taccctgctg
      181 aaggaggaac ttctcgggat cagggatgcc tctgaatcat cagaggagtc gctgtcagat
      241 acggcaaatg cactggctga aaatgcccgg aatgccggtg agctgggacg atttatgtcg
      301 gtggcccgtg tggcggcagg tggcggggtt gccgtactgg ccgcgcttgc tgccgccgcc
      361 tggcaggcag agcaggctga ccgggcctta ttgcgttcac tgatcctgac cggaggggcg
      421 gctgccacca caacggcaga attgtggaaa atggccgggg tgatcagcga tgaagccggt
      481 ggtggtatca gacaggcggc agaaaatctg gcccgtctgg cagaaagcgg gaaatatacc
      541 gccgggcagc tacggatcat gggggaaacc tctcagagat ggctgcagac ggtgggggac
      601 gatgccggga aggtggaaaa agcctttgaa gggattgcag cagatccggt gaaggcgctg
      661 gcctccctga atcagcagta taacttcctg agcgtttccc agttacgcca tattgatgag
      721 cttg
//
LOCUS       DAZFBR010000419          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFBR010000419 DAZFBR010000000
VERSION     DAZFBR010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.424"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            1..200
                     /locus_tag="ACHJZ2_005430"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACHJZ2_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            463..>709
                     /locus_tag="ACHJZ2_005431"
     CDS             463..>709
                     /locus_tag="ACHJZ2_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571290.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFE"
BASE COUNT          180 a          142 c          229 g          158 t
ORIGIN      
        1 aatatcaggc atacccgatt gacggcagcg gttttgagat gaacgggaag ggcagcagtg
       61 ccagaccgtc gctgacggtg tccaatcttt tcggccttgt caccgggatg gcggaggatt
      121 tgcagagcct ggtgggtgcc acggtggtcc gccgccgggt gtatgcccgt tttctggatg
      181 cggtgaattt tgtggcgggc ggtcgtggtt atcgggcggc agaggtaaaa agaataaaaa
      241 aatcccgcag agttgcgggg acagacaaag attaacgtta aggagttatt tttgtttctg
      301 gtgctcgggc aaaaaaacat taacgcagag aaattattag caccacagtc agtttgtgaa
      361 aatgtgaaga tattcagaag ttttattcag tcatgataca ggcatcctcc gggatgcctg
      421 ttgtttttgt gcgtaacagt tatcacagta aagggtgaga caatgggcaa aggtggcggc
      481 aaggcgcaca cgccggtaga ggcaaaggac aatcttaagt ccacgcagat gatgagcgtg
      541 attgatgcca ttggtgaagg gccgattgaa ggtccggtga aggggctgca gagtatcctg
      601 gtgaacaaaa ccccgctgac ggacacggac ggtaatcctg tgatacatgg tgtgacagcg
      661 gtctggcgcg ccggggagca ggagcagaca ccacctgaag gctttgagt
//
LOCUS       DAZFBR010000420          706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFBR010000420 DAZFBR010000000
VERSION     DAZFBR010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.425"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..617)
                     /locus_tag="ACHJZ2_005432"
     CDS             complement(<1..617)
                     /locus_tag="ACHJZ2_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4571291.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAENGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGSGSGDWRTDGFIVGVGY"
BASE COUNT          174 a          216 c          161 g          155 t
ORIGIN      
        1 ttataaccga cacccacgat gaaaccgtca gtgcgccagt cgccactgcc ggagccttca
       61 taagcaaggt caataaccac cgtctctacg ggactgaact gaatcccggc attccaggcc
      121 ggcgacagat gacgcgcagt atgaccatca ctggcggtgg tggtctcctt cacatacccc
      181 ggtttcactt catcacgccg gtaatcctga acactgtcag accagcgggt gtacgccatc
      241 ccggccatgc catagagact gacccgctca ctgagctgcc agacagggcc ggccatcaga
      301 ctgacataac gaccgcgcag gctttcataa tggaaggtat tttcacccgt cttcatcgtg
      361 tcacttttct tcaccgatgc ataactcagc gcgacaatgc cgcccaggtg atccgtgaac
      421 tcataacggt atttcacatt aatccctttt aaatcacctg cacgcgcacc ggtaccggac
      481 aatgccggta cgccgcccgg gtgaacctga gcatatccca cggaaaatgc accgtgtccg
      541 ttttcagcct gtgcaggaaa ggcaattcct gccagcaggg tagtaaacaa taatatcgtt
      601 gcgtataaat gccgcatgat tacctctttg ttttcagtca ataaaaaagg cacctcctga
      661 ggtgcccgtc cgggttaata aaccgtcagc tgatactgat ccctgc
//
LOCUS       DAZFBR010000421          694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFBR010000421 DAZFBR010000000
VERSION     DAZFBR010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.426"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            60..425
                     /locus_tag="ACHJZ2_005433"
     CDS             60..425
                     /locus_tag="ACHJZ2_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4571292.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSVSETASGMPD
                     PTLPEVSGGHGRADDIIGL"
BASE COUNT          150 a          167 c          205 g          172 t
ORIGIN      
        1 ccggtttttc ggtgacattt tctttcctct ggtgacaagc tatatggcga ggattttaca
       61 tggccgtgct tcgtacgtta ccgggcagaa tcaaaactct gaatacccgg cgggtgaatg
      121 tcctgaaggg ggaacagcgt cgggtcagtg gcagtgcccg ggtttccctc aagcgtcgta
      181 tctggctgag ggatgccgga cagtgctgtc tctgtgggcg tgtggttgac ctttgtgaca
      241 gtgaactcga tcaccgcatt gcacttcagt tcggtggtgg taatgaggag acgaatctct
      301 ggacgctctg taccgaatgc catcgccaga agtcagtcag tgaaacggcg agtggtatgc
      361 cggacccgac gctgccggag gtgtccggag gtcatggcag ggcagacgat atcatcggac
      421 tgtgacccgc cccggggggg gatcatccgg cgaaaaaacg atcgccccgg acaccgcccc
      481 ccgtctcatg cagagaaaaa attcctgttt caggccagtt aacatgttaa ctggctgccc
      541 gggcattttt tcggttttta tctttattat tcagtttgtt gtgcgaaaaa aatgttaact
      601 ggctttttca gcaaatgtta accaggcagc agttaacatt tgcggcatga gacgccggga
      661 aaaatgggct gaaccatacc cggctgagtg cgtt
//
LOCUS       DAZFBR010000422          692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFBR010000422 DAZFBR010000000
VERSION     DAZFBR010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.427"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..239
                     /locus_tag="ACHJZ2_005434"
     CDS             <1..239
                     /locus_tag="ACHJZ2_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO4571293.1"
                     /translation="TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQS
                     GDKGAYTCEVTDSAEQPQSITSDACTVTVNGAGG"
     gene            245..619
                     /locus_tag="ACHJZ2_005435"
     CDS             245..619
                     /locus_tag="ACHJZ2_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4571294.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
BASE COUNT          160 a          165 c          225 g          142 t
ORIGIN      
        1 ataccggtgc gctgctgaca atgtcagtca gtgtcaacgg gggaacgccg ccttataaac
       61 acgcctggaa gaaggatggt cagccggtag agggacagac tactgacact ttcagtaaag
      121 ccaatacgca gtcaggtgat aagggggctt atacctgcga ggtaacggat tctgcagaac
      181 agccgcagag cattacctct gatgcgtgta cagtaacggt taatggtgcg ggcggataag
      241 gcttatggca aaagatctga aaacactggc gctggccaga ctgtcggggt tccgtcataa
      301 aacggtgaag gtgccggaat ggagaaatgt cagcgtggtg ctgcgggagc cttcggcaga
      361 ggcctggtat ctgtggcagg aagtgctcaa tggtgatgga gaggatgacg ataccctgtc
      421 ggtggtggcg aaaacccgcc gtaacctgga agcggatgtg acgctgttct gcgatgtcct
      481 gtgtgatact gacctgcaac gggtgttcac tccggacgac cgtgagcagg tgctggccgt
      541 ctatggtccg gtacatgccc ggttgctgcg tcaggcactg gaactgatcg ctgatgcaga
      601 gtcggccaga aaaaagtagc ccgcccggaa attcgctttc tgatgcgact tgcgctccgt
      661 ctggggcgca ccttatccga actgcggcac ag
//
LOCUS       DAZFBR010000423          689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFBR010000423 DAZFBR010000000
VERSION     DAZFBR010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.428"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(83..>689)
                     /locus_tag="ACHJZ2_005436"
     CDS             complement(83..>689)
                     /locus_tag="ACHJZ2_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571295.1"
                     /translation="INITPMGIKIQETVSGNDRLFFYPTPLKNGCFILHNPLYSNSFP
                     SLYSHELFDLIEAYRNPTYSYLQNNIINRYIHTVSEYAGKDGIANMSLSIYAYSTCRF
                     WSKESVAVGDTIKLSNYEHIEFSFNSFSKDYFREQNSDIYKIFFKIASSSNSVVVDKN
                     TLTQKNFLENNTFFSITGIDESLYEKHIIFITLKVIVPSKK"
BASE COUNT          231 a          109 c          116 g          233 t
ORIGIN      
        1 cgcgaagtat gtcgtggcct ttggaacgtc tgctgatgct gggtaagatt agtcagcaaa
       61 ttccgcatac aggaaatcag cgctactttt ttgaggggac aataacctta agagtgataa
      121 agattatatg cttttcatag agagactcat ctatacctgt aatactgaaa aatgtattgt
      181 tttctaggaa gtttttttga gtgagcgtat ttttatccac aacaaccgaa ttactcgaag
      241 aagcaatttt aaaaaatatt ttatatatat cactattttg ttcacgaaag taatccttac
      301 taaatgaatt aaagctaaac tcaatatgct cataattaga caattttatt gtatctccta
      361 cagccacact ttcttttgac cagaaacgac atgtcgagta tgcgtatata gataatgaca
      421 tattggcaat accgtctttt ccagcatact cagaaacagt atgtatatat ctattgataa
      481 tattgttctg taaatacgaa tacgttggat ttctatatgc ttcaatcaaa tcaaataatt
      541 catgtgaata taaagaaggg aatgagttag agtaaagtgg attatgcaag ataaaacatc
      601 cattttttaa tggagtaggg taaaaaaaca acctgtcatt tccactgact gtttcctgta
      661 tctttatccc cattggggtt atatttatt
//
LOCUS       DAZFBR010000424          689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFBR010000424 DAZFBR010000000
VERSION     DAZFBR010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.429"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            230..622
                     /gene="ydeI"
                     /locus_tag="ACHJZ2_005437"
     CDS             230..622
                     /gene="ydeI"
                     /locus_tag="ACHJZ2_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO4571296.1"
                     /translation="MKFQAIVLASFLVIPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          207 a          131 c          147 g          204 t
ORIGIN      
        1 gtatgctcgt ccaatataat aattttcaaa catataaatc cgattattct gaaaatatgt
       61 ttaagactct tcaatcttct taactttagt gagttaaatt ctctgaatat ttgacagcta
      121 gttggctgat tttaaatgcc ttataacaat tggcagtatt cttatttggc aattataaaa
      181 atagcaacca gacttaataa gtcaaaccaa acgcaattaa ggagttagta tgaaatttca
      241 ggccattgta ttagcaagtt ttcttgtcat accttatgcg ttggcagatg accagggcgg
      301 tttaaaacag gatgcagcgc caccgccgcc tcatgcaata gaagatggct atcgcggaac
      361 cgatgatgca aaaaaaatga ctgttgattt cgcaaaaacc atgcacgatg gcgcttcggt
      421 ttcactacgc ggtaatttga tttctcacaa aggagaggac cgttacgttt ttcgcgataa
      481 gagtggtgaa attaatgttg ttattcctgc gaccgttttt gatggacgag aagttcagcc
      541 ggaccagatg atcaacatta gcggcagtct ggataagaaa agcgcgccgg ccgtcgttcg
      601 ggtcactcat ttgcagaaat aaaaataaat tagcctgatg gcctgaacta ccgggccatt
      661 tgggtactgg gttatgctat ttgtcatcc
//
LOCUS       DAZFBR010000425          685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFBR010000425 DAZFBR010000000
VERSION     DAZFBR010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.430"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..618
                     /gene="espW"
                     /locus_tag="ACHJZ2_005438"
     CDS             <1..618
                     /gene="espW"
                     /locus_tag="ACHJZ2_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO4571297.1"
                     /translation="RLKANTNIQQEQIADEVKSLTYLIPSEKKEMWIYKNNGKDNAKP
                     NLGERDVRMFENISSDDTDKITGRKFSELGEYLYSGNVIKLSQLSIRYLPNISSISLI
                     ETKQSLLLHRLYSDEVLQRNGTLIPTPLHEEKSIPADNIKTMLNNIPTYKMLPPFTET
                     QGNCSSGAATFLRKSGAEEKDILACSPRNYGLHHNIKTWDPLVRN"
BASE COUNT          276 a          119 c          116 g          174 t
ORIGIN      
        1 aggttaaagg ccaataccaa tatccaacaa gaacaaattg cagatgaagt aaaatcactt
       61 acatatttga ttccttcaga aaaaaaagag atgtggatat ataaaaacaa tggaaaagat
      121 aatgcaaaac caaacttagg agagcgagac gtaagaatgt tcgaaaatat tagttctgat
      181 gatacagata agataacagg aaggaaattt tcagagttag gtgagtatct ttattcaggg
      241 aatgtaataa aactcagtca gttatcaatt cgttacttac ccaatatcag ctcaatctca
      301 ttaatagaga caaaacagag tttgttgcta catcgattgt attcagatga agtacttcag
      361 agaaatggaa cgcttatccc gacaccacta catgaagaaa aatcaattcc agctgacaat
      421 ataaaaacaa tgctcaacaa cataccgact tacaaaatgt taccgccatt cacagaaaca
      481 caaggtaatt gttcttctgg cgcagccacg tttttacgca aatcaggcgc cgaagaaaaa
      541 gatattcttg catgtagccc ccgaaattat gggctgcatc ataacataaa aacatgggac
      601 cccttggtta gaaattaagg atccaaatat tatatatttc atataaagca agaaaaaaat
      661 attgtctgaa taattgttca gacaa
//
LOCUS       DAZFBR010000426          682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFBR010000426 DAZFBR010000000
VERSION     DAZFBR010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.431"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>682
                     /locus_tag="ACHJZ2_005439"
     CDS             <1..>682
                     /locus_tag="ACHJZ2_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4571298.1"
                     /translation="AHIPRNSSLNMNEISEKSLNEEEIKVLFRLLEIGGIENPFHKEV
                     QVRNRLIFTLLLNLGLRAGELLNLKIDDFDLRDNTLSVVRRHDSKEDGRSYQPLVKTG
                     ERVIPLSDELAREIFDYISNSREKMTKRKKHNFLFVAHCTGKNAGEPLSISAYEKVIS
                     TLKRASPELYNLSGHRLRHSWNYMYSKGIEGAELEYNRRKDLRNYLMGWSKESIMCDR
                     YNYKYISQQ"
BASE COUNT          258 a           93 c          143 g          188 t
ORIGIN      
        1 gcacatattc caagaaacag ttcattgaat atgaatgaaa tatctgaaaa atcgttgaat
       61 gaagaagaga tcaaagtgct tttccgtcta ttggaaattg gcgggattga aaaccctttc
      121 cataaggaag tacaggtcag gaacagacta atatttacct tgctcttaaa tttaggttta
      181 agggctggag agttgctcaa tcttaaaata gatgattttg atctcagaga caacacttta
      241 agtgtagtca gaagacatga ttcaaaagaa gatggaagat cttatcaacc attagtaaag
      301 actggagaaa gggttattcc gttatctgat gagttagccc gtgaaatttt tgattacata
      361 agcaatagca gagaaaaaat gacaaaaagg aaaaagcata atttcttatt tgtggctcat
      421 tgcaccggaa aaaacgcagg agaaccctta agcatttcag cttatgagaa ggtgatatca
      481 acactgaaac gagcaagtcc agagctatat aatctttcag gccataggtt aaggcactct
      541 tggaattata tgtattctaa aggaatagaa ggtgctgaat tagaatataa tagaaggaaa
      601 gatttaagga attatttaat gggttggtca aaggagtcaa taatgtgtga tagatacaac
      661 tataaatata tatcgcaaca ag
//
LOCUS       DAZFBR010000427          680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFBR010000427 DAZFBR010000000
VERSION     DAZFBR010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.432"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            269..>680
                     /gene="tccP"
                     /locus_tag="ACHJZ2_005440"
                     /gene_synonym="espF(U)"
     CDS             269..>680
                     /gene="tccP"
                     /locus_tag="ACHJZ2_005440"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HFO4571299.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     AEHIPPAPNWPAPPPPVQNEQSRPLPDVAQRLVQH"
BASE COUNT          209 a          164 c          119 g          188 t
ORIGIN      
        1 cttaagagta gatttttggc attttgggaa gatcagcggg acataagaga catacaacac
       61 aggaggaatg gctgtttttt aatagtgctt tgaattccgc atgaaacctt tcttagcagt
      121 cgaaaagcaa aacatacgtt ttctttttaa caataattga tgagtattgc atcgagtgtc
      181 atttatacat cgccgcattt atttgccact ataaaaaggc tgattacata tctatcggag
      241 aactaaaatt acttataagg taatctgcat gattaacaat gtttcttcac tttttccaac
      301 cgtcaaccgc aatattacag ctgtatataa aaaaagcagc ttctctgtat caccacagaa
      361 aatcacatta aatcctgtaa aaatcagctc acctttttca ccaagcagta gctccatcag
      421 cgcaacaact ctctttcgag ccccaaacgc ccattcggca tcatttcatc gacagtctac
      481 tgctgaaagt tcgttacatc aacaacttcc taatgtgagg cagcgcctga tacaacatct
      541 tgcagagcat ggcattaaac ctgcccggag tatggctgaa catattcctc cggcacctaa
      601 ctggcctgcg ccaccaccgc cagtacaaaa tgaacaatca agacctctgc ctgatgtggc
      661 tcagcgtctg gtgcagcatc
//
LOCUS       DAZFBR010000428          678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFBR010000428 DAZFBR010000000
VERSION     DAZFBR010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.433"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            85..510
                     /locus_tag="ACHJZ2_005441"
     CDS             85..510
                     /locus_tag="ACHJZ2_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4571300.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            507..581
                     /locus_tag="ACHJZ2_005442"
                     /pseudo
     CDS             507..581
                     /locus_tag="ACHJZ2_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          195 a          165 c          164 g          154 t
ORIGIN      
        1 taagcgtaaa ctgaccgccg tatgtagcca tcagacgaaa attggtaact tagacgccca
       61 tctgatatag acggacatct aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa
      121 aactattcga atgaattcaa acttcgtatg gtggaactgg catcacaacc aggagcctct
      181 gttgcccgta ttgcccggga acacgatatc aatgataacc tcctgttcaa atggctcagg
      241 ctctggcaga acgaagggcg tatatcacgg cgtcttcagg taacgacctc ttctgacact
      301 ggcgttgaat tattacctgt agaaataacg ccggatgagc agaaagaacc ggtggcggcc
      361 attgcgccgt ctttatccac ttccactcag accagtgtca gtgccggctc ctgcaaggtg
      421 gagttccgtc acggtaacat gacgctggaa aatccatcgc cagagctgct cacgctgttg
      481 atccgtgaac tgaccgggag gggacgatga tctcactccc gtcaggtacc cgcatctggc
      541 tcgttgccga cgttaccgat atgcgtaaat ccttcaacgg atacgacata tgctgaagga
      601 ggagcagtaa tggattaagt gataacaaat acccagaaat agaggttcaa atccagctta
      661 gttagtacat aaatacta
//
LOCUS       DAZFBR010000429          676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFBR010000429 DAZFBR010000000
VERSION     DAZFBR010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.434"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(14..343)
                     /locus_tag="ACHJZ2_005443"
     CDS             complement(14..343)
                     /locus_tag="ACHJZ2_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4571301.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(340..>676)
                     /locus_tag="ACHJZ2_005444"
     CDS             complement(340..>676)
                     /locus_tag="ACHJZ2_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4571302.1"
                     /translation="GKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGG
                     AGSPAQMRRAEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDELRLQLRD
                     GGMLSGSGR"
BASE COUNT          139 a          250 c          142 g          145 t
ORIGIN      
        1 tgaatatcct gcatcagttc accacctgct taaactccgc gctgaactca acgcgcaaca
       61 tcccgacccg cgcagaccac ccggcacagg tcacctttat ctgccggtat gcatagggtg
      121 gcttccacaa aaatgccttc cagcctccgt gctctgccag gaacgcttcc agatgccggg
      181 cctcctcccg ggtcacggaa agcgtcacac ggtatgtttt caggtcagca ttcagccctg
      241 ccgccatacg ctgtgagtac ccgtcaccaa aacgcacttc acgcaccgat ggctgcgagt
      301 tcacctccat atccggcttc actttccagc gaaaggtttt catcgcccgc tccccgataa
      361 cataccgcca tcacgcaact gcagccggag ctcatcctgc gcccccttgc gggccatgtc
      421 atacaccgcc ttcatcagct gcggccccgc ctgtccgttg gtgccgtcgt tctgaatcac
      481 cacgtgattg ttctgattaa aattaatacc ttccgcccgc cgcatctgcg ccggacttcc
      541 ggcaccaccc acataaccac cttccgcata gccgcgcatc agacggtaaa gattccccac
      601 acctatccgg ctggttgcct ctttcgtgaa aacaaactcc ccgcggtgaa ctatccccgc
      661 aggctcatat ttgccg
//
LOCUS       DAZFBR010000430          674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFBR010000430 DAZFBR010000000
VERSION     DAZFBR010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.435"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>674)
                     /gene="sepL"
                     /locus_tag="ACHJZ2_005445"
     CDS             complement(<1..>674)
                     /gene="sepL"
                     /locus_tag="ACHJZ2_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFO4571303.1"
                     /translation="PLINLQNELAMITSSSLSETIEGLSLGYRKGSARKEEEGSTIEK
                     LLNDMQELLTLTDSDKIKELSLKNSGLLEQHDPTLAMFGNMPKGEIVALISSLLQSKF
                     VKIELKKKYARLLLDLLGEDDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENG
                     ASLLDWFMEIKDLPEREKHLKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLS
                     LDNYAD"
BASE COUNT          200 a          155 c          103 g          216 t
ORIGIN      
        1 atatctgcat agttatcaag tgataaaaaa atgattaccc tgcataaatc actaataatt
       61 gaagatgtct ttactttgtc ttcaaaagaa gacatataag agagatcgaa cgacagcgcc
      121 ctaataatga cttttaagtg cttctcacgc tcgggaagat ccttaatctc cataaaccag
      181 tcaagtaaag aggcgccatt ttcagaatcc tcatccttag ccttttcata aagcttcttg
      241 atcttctgga taccttcctg atttaactca cccaccccta accaggaaag cagagccagc
      301 tcccaatcat cttcgcctaa taaatctaat aataacctgg catatttctt ttttaattca
      361 atcttaacaa acttagattg caataaagaa gatattagcg caacaatttc cccctttggc
      421 atgttgccaa acatcgccaa agtaggatcg tgttgttcca gcagcccgct gttttttaat
      481 gatagttctt ttattttatc actatcggta agagtaagca gctcttgcat atcattgagc
      541 agcttctcga ttgtcgagcc ttcctcttct ttccttgcgc tacctttgcg atatcccagg
      601 cttagccctt caatcgtttc agaaagcgat gaactagtaa tcattgctaa ttcattttgc
      661 aaatttatta atgg
//
LOCUS       DAZFBR010000431          665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFBR010000431 DAZFBR010000000
VERSION     DAZFBR010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.436"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>665)
                     /locus_tag="ACHJZ2_005446"
     CDS             complement(<1..>665)
                     /locus_tag="ACHJZ2_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFO4571304.1"
                     /translation="PAKKYLEHGLFSEYRQDGTIPYVVSTLNISGPDMIMRQMKKYYK
                     SLGRIGEVHIKDNKLSDVNFLGVYASSNKDNKSFNWLNPVSVGINDITPDDESSWAVR
                     NNDINKILFEKINCHVPEKLPTSLYYEIDSRSFFQGWDNKSIKHVTEINKDLIKDINL
                     LLTSSNIDVKLLIKLDRELYAISSKIDNPLALRSIRTLQLQLANYVTSNTFEPENTIN
                     FIY"
BASE COUNT          209 a          111 c           99 g          246 t
ORIGIN      
        1 ataaataaaa ttaattgtgt tttcaggttc aaatgtattc gaagttacgt aattagctaa
       61 ttgtagctgt aaggttctta tagagcgcaa ggccaacgga ttatctattt tgctgctaat
      121 cgcatataat tcacgatcca gttttattaa aagtttaaca tcaatattag acgatgttaa
      181 taataaatta atatctttga tgaggtcttt attaatctct gtaacatgct ttatgctttt
      241 attatcccac ccctgaaaaa aagaccgcga atcgatttca tagtataggg aggtaggtag
      301 tttttcgggt acatggcagt ttattttttc gaatagaatt ttatttatat cattatttct
      361 gacagcccat gagctttcat catctggtgt aatatcatta ataccaacac tcactggatt
      421 tagccagtta aaacttttat tgtctttatt tgaacttgca tatacaccca aaaaattaac
      481 atcactcaat ttattatctt taatatgcac ttcacctatg cgcccaagag atttatagta
      541 ttttttcatt tgtcgcataa tcatgtctgg gccagatata tttaaagtgc taaccacata
      601 aggaattgta ccgtcctgtc tatattcaga aaacaaaccg tgttctaaat atttctttgc
      661 aggta
//
LOCUS       DAZFBR010000432          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFBR010000432 DAZFBR010000000
VERSION     DAZFBR010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.437"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            7..>660
                     /locus_tag="ACHJZ2_005447"
     CDS             7..>660
                     /locus_tag="ACHJZ2_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO4571305.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTKCHGESLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLREQNTDCHDESRRVTHMSRQVTTDTDTDKE
                     LNPTHNARMRESAPASESNGAPLQTAEPEYPDGLSEPIGKFPMTGVWRPSLDFRQRAA
                     "
BASE COUNT          180 a          154 c          197 g          129 t
ORIGIN      
        1 gtgagtatgg ctaatgcctg gctcagattg tggcatgaca tgccaaatga tcccaaatgg
       61 cgaaccattg ccagggtctc aggacagcca atcgcaacag tgatggcggt gtatatccac
      121 cttctggtga gtgcgtcacg aaatgtcacg aaatgtcacg gcgagtcact acgtggtcac
      181 attgatgtca cgacggaaga tttagcaagt gcgcttgatg tgacggaaga cgtgattgat
      241 tcaattttgc atgcaatgca ggggcgagtt ctggatggcg atcttatttc cggatgggaa
      301 aaacgccagg tgatgaagga ggataacggt aatgtttcgc aaaccgcaaa atccccggca
      361 gagcgcaaga gagcgcagcg ggagcgggaa agactgcggg agcagaacac tgattgtcac
      421 gatgagtcac gacgcgtcac gcatatgtca cgacaagtca cgacagatac agatacagat
      481 aaagaattaa accccacaca taacgcgcgc atgcgcgaga gtgctccagc cagtgagtcg
      541 aatggcgcgc cgttgcagac agcagaacct gaatacccgg atggcctgag cgaaccgatc
      601 gggaaatttc cgatgactgg tgtctggcgg ccgtcgctgg attttcgaca gcgggctgca
//
LOCUS       DAZFBR010000433          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFBR010000433 DAZFBR010000000
VERSION     DAZFBR010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.438"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            8..>660
                     /gene="espL"
                     /locus_tag="ACHJZ2_005448"
     CDS             8..>660
                     /gene="espL"
                     /locus_tag="ACHJZ2_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE stator protein
                     EspL"
                     /protein_id="HFO4571306.1"
                     /translation="MKLYEFKNIKIDLVLTEDIIPEDKLQEIIQSDDIIKLARKKTYE
                     HLLRARRKSKELKTESRKKIARKMILMRERIRKNNKIKLDKEVNQSIKWVKDIQAIEL
                     VLMQDIMNKIHLSLTNALHSLDTSSRINWDDLLNEVVRETLSNNNIVGAIKITKNPDI
                     KLDPGEANNIQLINDANTPHNKIIIENEYIRITLDPLEQISILLNSFKDNYLSIIQE"
BASE COUNT          281 a           84 c          107 g          188 t
ORIGIN      
        1 ttgccttatg aaactttatg aatttaaaaa tataaaaatt gatttagtgc ttacagaaga
       61 tatcatacca gaggataagt tacaggaaat tattcaatct gatgatatta tcaaactggc
      121 tcgaaaaaaa acatatgaac atttactgag ggcgaggagg aaatcgaaag aattaaagac
      181 agaatccaga aaaaaaattg ccagaaaaat gattctaatg agagaaagga ttcgaaaaaa
      241 taataaaata aaattagata aagaggttaa ccaatcgata aaatgggtta aagatatcca
      301 ggctatcgag ttggtcttaa tgcaggacat aatgaataag attcatttgt cactgactaa
      361 tgcattacat tctttagata catcatcaag aattaattgg gatgatttac ttaatgaagt
      421 tgtaagggaa actctatcta ataacaatat tgtcggagct attaagatca ctaaaaaccc
      481 agacattaaa cttgatcctg gcgaggccaa caatatacag cttattaatg atgcaaacac
      541 accgcataat aaaattataa tagaaaatga atatattaga ataactttag acccattaga
      601 gcaaataagt attttattga attcatttaa agataattat cttagcatta ttcaggaatg
//
LOCUS       DAZFBR010000434          659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFBR010000434 DAZFBR010000000
VERSION     DAZFBR010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.439"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            28..>659
                     /gene="espJ"
                     /locus_tag="ACHJZ2_005449"
     CDS             28..>659
                     /gene="espJ"
                     /locus_tag="ACHJZ2_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     ADP-ribosyltransferase EspJ"
                     /protein_id="HFO4571307.1"
                     /translation="MSIIKNCLSLINNALNIQKTSYSLTKMEQAGKLLNRKITPENTP
                     PMLLSYRNADLTQEKNITERVLSIFKIKRDFVAVRIQNNQFTDLKNKKIQGHQNTVAS
                     VMDWYNPQKNALGITMGTPRKSADIAKEEHRNALNFMIMEKNTFHEKILNSNDNLQKS
                     YSKTEDSSWVAASVGSLLDKGAKVYPDTSCSLRLGEPFIFTLPESVRVDV"
BASE COUNT          272 a          110 c          108 g          169 t
ORIGIN      
        1 catttatatg agaaaaaggc atttataatg tcaattataa aaaactgctt atcattaatt
       61 aacaatgcat taaatataca aaaaacatca tattctctga ccaaaatgga gcaggcaggg
      121 aagttgttaa atagaaaaat aactccagaa aatactcctc caatgttgtt atcatacaga
      181 aatgcagatt taacgcaaga aaaaaacatt acagaaagag ttttatccat atttaaaata
      241 aaacgagatt ttgttgctgt aagaatccaa aacaatcagt ttactgattt gaaaaacaaa
      301 aaaattcaag gccatcaaaa cacagttgcc agtgtcatgg attggtataa tccccaaaaa
      361 aatgctttgg gtataactat gggtacacca agaaaatcgg ccgatatagc aaaagaagag
      421 cacagaaatg cactaaattt catgataatg gaaaaaaaca catttcatga aaaaatctta
      481 aacagcaatg acaatttgca aaaatcatac agcaaaacag aagacagtag ttgggtcgca
      541 gcttctgtcg gctctcttct agataaagga gcaaaagtat atcccgatac atcatgctct
      601 ctgagactgg gagaaccatt catttttacg ctgccagaat cagttagagt cgatgttga
//
LOCUS       DAZFBR010000435          658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFBR010000435 DAZFBR010000000
VERSION     DAZFBR010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.440"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..132
                     /locus_tag="ACHJZ2_005450"
     CDS             <1..132
                     /locus_tag="ACHJZ2_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4571308.1"
                     /translation="SRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            83..>658
                     /gene="traT"
                     /locus_tag="ACHJZ2_005451"
     CDS             83..>658
                     /gene="traT"
                     /locus_tag="ACHJZ2_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO4571309.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTE"
BASE COUNT          192 a          150 c          172 g          144 t
ORIGIN      
        1 agccgggatg aggccatctc agtaatacgg gaatacattg agattttcta caatcgtcag
       61 cgtcgtcact ctcgtctggg gaatatctcc ccggcagcct tcagggaaaa atatcatcag
      121 atggctgctt aaaaaaagaa caaatggtag tgtccgctat tgtcagtaca cctcaaccat
      181 ctggcttgaa cctgcgagcg aacgcaccgt tttcctgcag atcaaaaaca cgtcggataa
      241 agacatgagt ggactacagg gaaaaattgc cgatgcagtc aaagccagag gttatcagat
      301 catgacttcc ccggataaag cttactactg gatccaggct aacgttctca aagcagacaa
      361 aatggatctg cgtgaatctc agggatggct gagtcgcggt tatgaaggtg cagcagtcgg
      421 agctgctctt ggaggtggaa tcacagcgta caataccggg tcggcaggta ccactctcgg
      481 tgttggactg gctaccggtc tgattggcat ggccgcggat gcaatggtgg aagatataaa
      541 ttacacaatg attacagatg ttcagattgc cgagcgaacc agaaccagcg tcagaacgga
      601 taatgttgca gctttgcgtc agggaacatc aggctccaaa attcagacaa gcacagaa
//
LOCUS       DAZFBR010000436          650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFBR010000436 DAZFBR010000000
VERSION     DAZFBR010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.441"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..626)
                     /locus_tag="ACHJZ2_005452"
     CDS             complement(<1..626)
                     /locus_tag="ACHJZ2_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO4571310.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDES"
BASE COUNT          222 a          124 c           87 g          217 t
ORIGIN      
        1 attgactcat ctgataatag atcaaagaat ttttccgtta gtctttgttt taaccattcc
       61 cgtaaattaa tatacattac accagagtta aaatagctac cgttaaaatc ctcattacac
      121 aaacgctcag cgcttttact ttgcatagca tctacatcaa ttaccactgc cccatattca
      181 tctttaaact ctaatgctat taactcgttc aatgaaccct tacagacgat atcagcatca
      241 agatataata atgaatccaa tcgcgcagaa aaataatcaa atgaaagcaa acgataataa
      301 atagatactg gccagatatt tgattgaggt agcgccttca atggctcaga atcaatatga
      361 tataatttaa ttactgtttg atacttcgtt gctaattgtg ataataattc aatatactct
      421 ttgtctaagt aatcagcgat tatgtgaaag gtaaatttgt tttgtttatt attttcaaga
      481 attgaagcaa ttgaaatcgc agccccaacc tggtagttct catcaatacc ccatgagaca
      541 ttaaacgttt cccgttcatc aagtttaacg gggcgcttgt ccagttcaat tatatcttta
      601 aattgagtaa gatgtttaaa atccaattta attacccttc attataatca
//
LOCUS       DAZFBR010000437          650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFBR010000437 DAZFBR010000000
VERSION     DAZFBR010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.442"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>650
                     /locus_tag="ACHJZ2_005453"
     CDS             <1..>650
                     /locus_tag="ACHJZ2_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO4571311.1"
                     /translation="SKFTWETYGDIVVSPEDRISHLELDGFNYFSQPELDTPISDSFS
                     YLYDNFQIVDSDVHIKLLHLNRETKQITPHRIILKRYFIDSFAKTSITDREKNIYPVI
                     CDSPDHFTSDIYRHPFRIVLGNKTLYPSEELVKFISTSKEYLSNMDVINNVIVPQKTT
                     KKNKLSIVSLNSNIKNDIVLSGVMTGTSKIFHLNNSGDLLLTTSKTHGGGVVVIFK"
BASE COUNT          238 a           99 c          102 g          211 t
ORIGIN      
        1 taagtaaatt tacatgggaa acgtatggtg atatcgttgt atcacctgaa gatagaattt
       61 ctcatctgga gttagatggg tttaattatt tttcacaacc cgagttagat actccaatat
      121 ctgactcatt ttcatattta tatgataatt ttcagatcgt tgatagtgat gtgcatatta
      181 agcttcttca tctcaacaga gaaacaaagc aaataacacc acatcgtatt attcttaaaa
      241 gatactttat agactcattt gctaaaacaa gtatcactga cagagaaaag aatatatatc
      301 ctgtgatttg tgattcacct gatcacttca caagcgacat atataggcat ccatttagaa
      361 tagttctcgg gaataagaca ttgtatccat cagaagaact tgtaaaattc ataagcacct
      421 caaaagaata tttaagcaat atggatgtga ttaacaatgt tattgttcct caaaaaacaa
      481 caaagaaaaa caaattatcc atagttagtt taaatagcaa tatcaaaaat gatattgtat
      541 tgtccggagt aatgaccgga acatcaaaga tttttcatct aaataattca ggtgatttat
      601 tgttaacaac ctcaaaaact catggtggtg gggttgtggt tatttttaaa
//
LOCUS       DAZFBR010000438          641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFBR010000438 DAZFBR010000000
VERSION     DAZFBR010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.443"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>641
                     /locus_tag="ACHJZ2_005454"
     CDS             <1..>641
                     /locus_tag="ACHJZ2_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFO4571312.1"
                     /translation="NKDDYEKYDNKSILISAKILSNMIKNPIVFLGYSLTDRNVKKLL
                     SDFSSQLPREDGRKSAERIILIEYKENEQEVVPKQITDQQLQITYTSVKTDNYKQIYD
                     EISAVDEGLSPYDVLRYQRAIKTLIINEGEKGHLDTLLVSPSDLDRLEESVKQGKNLV
                     VALGDKKYVFTQVKEINYLEDYLFNKNEISNKLAIDFILGSVNSLQLPFSKTIT"
BASE COUNT          259 a           95 c           95 g          192 t
ORIGIN      
        1 aataaagatg attacgaaaa atatgacaac aaatctatac tcattagtgc caagatacta
       61 tctaacatga ttaaaaatcc aatagttttt cttggatatt ctttaactga caggaatgta
      121 aagaaacttc tttctgattt ttcctcacaa ctacctagag aagatggtcg aaaatcagct
      181 gaaagaataa ttttgattga atataaagag aatgaacaag aagtagtacc aaaacagatt
      241 accgatcaac agctacaaat tacatataca tctgttaaaa cagataacta taaacagatc
      301 tatgatgaga tcagtgctgt tgatgaaggg ctctcacctt atgatgtttt aaggtatcaa
      361 agagccatta aaacacttat cattaatgag ggtgaaaaag ggcacttaga tactctattg
      421 gtttcacctt ctgacttaga cagacttgaa gaaagtgtta aacaaggaaa aaatttagtt
      481 gttgctctag gcgataaaaa gtatgtattc acacaagtta aagaaataaa ttatcttgaa
      541 gactacttat ttaataaaaa tgaaatatct aataaattag ccatagattt catattaggt
      601 tcagtaaact cattacaact tccattttca aaaactataa c
//
LOCUS       DAZFBR010000439          633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFBR010000439 DAZFBR010000000
VERSION     DAZFBR010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.444"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>633)
                     /gene="nleL"
                     /locus_tag="ACHJZ2_005455"
     CDS             complement(<1..>633)
                     /gene="nleL"
                     /locus_tag="ACHJZ2_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO4571313.1"
                     /translation="HDKVRAAEELIKKISLDELAAFRPYVKMSLADSFSIHPYLNNAN
                     IQQWLEPICDDFFDTIMSWFNNSIMMYMENGSLLQAGMYFERHPGAMVSYNSSFIQIV
                     MNGSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVL
                     LSSQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNL"
BASE COUNT          218 a          142 c           88 g          185 t
ORIGIN      
        1 aagattgctc aatgaatacc caaatgtatt ttgaacttct ccatccttat ataaaaaaaa
       61 ggacatccaa ttagttgatg tattaggaga taacatatca accatatgac tcaaagaaca
      121 catcattgcc ataccatcat cctgtgatga taacaaaacc cagttataag ccaaatcgga
      181 atcatcatcc cagtcaggct tcccagagcc atcgcacaat ccaaaatcac tctgctgtgt
      241 gacaggataa actttttcat tttttaaata tacttcatag agttccctaa atcgttcctg
      301 cattccatca cgccgtgaac cattcattac aatttgtata aaggaactat tataagatac
      361 catcgcacct ggatgtcgct caaaatacat ccctgcctgc aataaactac cattctccat
      421 atacatcatt attgaattat taaaccaaga cattatagta tcaaaaaagt catcacatat
      481 aggctcgagc cattgctgta tatttgcgtt gttcaaataa ggatgaatac taaatgaatc
      541 agccaaagac atcttaacat agggccggaa cgccgctaat tcgtcaagag atattttttt
      601 gataagttct tcagcggccc ttactttatc atg
//
LOCUS       DAZFBR010000440          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFBR010000440 DAZFBR010000000
VERSION     DAZFBR010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.445"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(290..496)
                     /locus_tag="ACHJZ2_005456"
     CDS             complement(290..496)
                     /locus_tag="ACHJZ2_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO4571314.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          169 a          147 c          123 g          189 t
ORIGIN      
        1 ccatactgcg tcatgtcgcc ctgacgcgcc gccggttttc cgctcatctg ctcatccttg
       61 ttttatcggg ttatttgtct gcttccaggc aaaaccctac agatttataa gaaagcctta
      121 agtgagatat ccgtcagata gaaacaatta ggaaaatatt tatatatatt tcatgaatat
      181 atgtttcgat tggtataacg gtgtctaaat attcctctga acatcttcct gatacaaaaa
      241 accgccagcg tttccgctgg cggttttcaa tgtattcaaa actcagcaat cagtaaaaat
      301 caccggttgc cttttcggcc tgatccatcc aaaccggttt atcgctggtt ttcgaccaga
      361 cgcgatgcag gtagctgtaa aagcgggcgc ggtctttcca gaaaagcatg aaaggcagcg
      421 ccagaacgcc cgctgcaacc gcataagtac ggcgtaaaaa gataagccat gcaggatatt
      481 ctctgtagag ttccatactt ttctccccct tttgtatcta cccggtgaat agcaccggaa
      541 aaatgaattt gtttatctga tgaaaatagt accgcctttt gtgtaatttt actactcatc
      601 cgaccactta tttttgctta ttgatggt
//
LOCUS       DAZFBR010000441          618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFBR010000441 DAZFBR010000000
VERSION     DAZFBR010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.447"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..602
                     /locus_tag="ACHJZ2_005457"
     CDS             <1..602
                     /locus_tag="ACHJZ2_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO4571315.1"
                     /translation="AGFNELYRNGGITEAACWAHARRKIHDVHVRIPSALTEEALEQI
                     GQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLSRHSELAKAFAYAL
                     NQWPALTYYANDGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTC
                     KLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT          151 a          161 c          187 g          119 t
ORIGIN      
        1 acgccgggtt caacgagctg tatcgcaatg gtgggataac ggaagctgcc tgctgggctc
       61 atgcccgccg aaagatccac gatgtgcacg tccgcatccc gtcagcactg acggaagaag
      121 ccctggagca gatcggtcag ttgtacgcca tagaggcgga tataagggga atgccggcag
      181 agcagcggct tgctgaacgt cagcgaaaaa cgaaaccgtt gttgaaatcc ctggaaagct
      241 ggttgcgtga aaagatgaag accctgtcgc gacactcaga gttggcgaag gcgttcgcgt
      301 acgcacttaa ccagtggccg gcactgacgt actatgcgaa cgatggctgg gtggaaatcg
      361 acaacaacat cgctgaaaat gccctgcggg cggtcagtct gggtcgtaaa aacttcctgt
      421 tcttcggctc tgatcatggt ggtgagcggg gagcgctact gtacagcctg atcgggacgt
      481 gcaaactgaa tgacgtggat ccagaaagct accttcgcca tgtgcttggc gtcatagcag
      541 actggccggt caaccgggtc agcgaactgc ttccgtggcg catagcactg ccagctgaat
      601 aacacatccc cgtcaata
//
LOCUS       DAZFBR010000442          618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFBR010000442 DAZFBR010000000
VERSION     DAZFBR010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.448"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>618)
                     /locus_tag="ACHJZ2_005458"
     CDS             complement(<1..>618)
                     /locus_tag="ACHJZ2_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO4571316.1"
                     /translation="INLYNEIRKKYNNNPSGQQEYLNKLRELYATYQKISTPLKHMFN
                     SFFLENMIKLQDNFFNYCIVKGVTEINDELRINYLKNVIKLSDDDIGNYQKTINDNKD
                     RVKKLILDLQKQFGENRISIKDVNSLTSLSKSENNHNYQTEMLLRWNYPAASDLLRMY
                     ILKEHGGIYTDTDMMPAYSKQVIFKIMMQTNGDNRFLEDLKLRRAI"
BASE COUNT          179 a           90 c          104 g          245 t
ORIGIN      
        1 tattgcacga cgtagtttca aatcttccaa gaaacgatta tctccgtttg tctgcatcat
       61 aattttaaaa attacttgtt tagagtatgc aggcatcata tctgtgtctg tataaatacc
      121 accatgctct ttaaggatat acatcctgag caggtctgag gcggcaggat agttccatcg
      181 aagcaacatt tcagtttgat aattgtgatt attttctgat tttgaaagag aggttaaaga
      241 gttaacatct ttaattgaaa tgcggttttc accaaattgt ttctgtaaat caagaattag
      301 tttttttact ctatctttat tgtcgttaat tgttttctga taattaccaa tgtcatcgtc
      361 tgacagtttt attacatttt taaggtagtt tattcgtaac tcatcattaa tctctgtaac
      421 acctttgaca atgcaatagt tgaagaaatt atcctgaagt ttaatcatgt tttctaaaaa
      481 aaatgaatta aacatgtgtt tcagaggggt ggatattttt tgataagtag cataaagctc
      541 tctgagtttg tttaagtact cttgttgtcc cgatgggttg ttgttgtatt tcttccttat
      601 ttcattatat agattaat
//
LOCUS       DAZFBR010000443          616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFBR010000443 DAZFBR010000000
VERSION     DAZFBR010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.449"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>616
                     /locus_tag="ACHJZ2_005459"
     CDS             <1..>616
                     /locus_tag="ACHJZ2_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFO4571317.1"
                     /translation="NGCDVILESDTIQKIKSFAKNITIQTADGVMANWDDKLNTLSVY
                     SIDMIAWRDKNKTAKEPLPVDVIQLTNWRMHNTCSLFYDKYQVDIEANKLTYTVLFPD
                     TELPVQLNYTSIIYGNHGAKYTFLNSGSKTIDIHILDKNSDYDTFDFRNIIFEHYTNE
                     IFISFDNQGGFVISILNNATSEAANINVFRKNMTSLDSSGSLIY"
BASE COUNT          229 a           81 c          104 g          202 t
ORIGIN      
        1 ataatggatg tgatgtgatt ttagaaagtg acactataca aaaaataaaa tcatttgcaa
       61 aaaatataac aatacagaca gcggatggag ttatggcaaa ttgggatgat aaactaaata
      121 ctctgtctgt ttattcaata gatatgattg catggaggga taaaaataaa actgcaaaag
      181 aaccgttacc agttgatgta atccagttaa ctaattggag gatgcataac acatgctctt
      241 tattttatga taaatatcaa gttgatattg aagccaataa gttaacgtat acagtcttat
      301 ttcctgatac ggagttaccc gttcagctca attataccag tattatttat ggtaatcatg
      361 gggcaaaata caccttcctc aattcaggaa gtaaaactat agatattcac attcttgata
      421 aaaatagtga ttatgatact tttgacttcc gtaatattat ttttgaacat tacaccaatg
      481 agatatttat aagttttgat aatcaaggag gatttgttat atcaatatta aataatgcta
      541 cgagtgaagc ggcgaacatt aatgtattcc gaaaaaacat gacttcttta gatagtagtg
      601 gttcattgat atattt
//
LOCUS       DAZFBR010000444          614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFBR010000444 DAZFBR010000000
VERSION     DAZFBR010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.450"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>614
                     /gene="yjgL"
                     /locus_tag="ACHJZ2_005460"
     CDS             <1..>614
                     /gene="yjgL"
                     /locus_tag="ACHJZ2_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO4571318.1"
                     /translation="NSQELNNSQELNNSQDLKNSQVSCKGSVDFTITDLLEKSLNNAL
                     LAIRNEHLLLMPHVCSESISYLLGENGILEEIDKLYELNDHGIDNDKEGNNEINDIMI
                     NLSHILIESLDDAKINLTPVIHSMLMTFLELPYNNDVKILEWCFNKSMQYFDDSAKIE
                     HACSVINHINFRRDQSKVAETLFFNLDKEPYKNSPELQELIWKK"
BASE COUNT          224 a           85 c          112 g          193 t
ORIGIN      
        1 ataattcgca ggagttaaat aattcgcagg agttaaataa ctcgcaggac ttaaaaaatt
       61 cgcaggtgag ttgtaaaggt tcagttgatt ttacgattac ggatttatta gaaaaatcat
      121 tgaataatgc attattagca ataaggaacg aacatctgct attaatgcct catgtatgta
      181 gtgaatcgat ttcatactta ctgggcgaaa atggtatact tgaagaaata gataagctct
      241 acgaattaaa tgatcacgga attgataatg acaaagaagg taacaatgaa attaatgaca
      301 tcatgattaa cctgtctcat attcttattg aatccttaga tgatgcaaag attaatctta
      361 caccggtcat ccattcgatg ttgatgactt ttttagaatt gccatataat aatgatgtaa
      421 aaatactgga gtggtgtttt aataaaagca tgcaatattt tgatgattct gcaaagatag
      481 agcatgcatg ctccgtaata aatcatatta attttcgtcg cgatcagtct aaagtagctg
      541 agacattatt tttcaatctc gataaagaac cctataaaaa tagccctgaa ttacaggagt
      601 tgatttggaa aaag
//
LOCUS       DAZFBR010000445          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFBR010000445 DAZFBR010000000
VERSION     DAZFBR010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.451"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..198
                     /locus_tag="ACHJZ2_005461"
     CDS             <1..198
                     /locus_tag="ACHJZ2_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4571319.1"
                     /translation="MLANTCMWTYRSDECGYTGGAVADEFDNPTTDIRKDRCSKCMRG
                     CEMRSMVANFGGFLSINKLSQ"
     gene            209..>608
                     /locus_tag="ACHJZ2_005462"
     CDS             209..>608
                     /locus_tag="ACHJZ2_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4571320.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAG"
BASE COUNT          119 a          147 c          205 g          137 t
ORIGIN      
        1 atgctggcga atacctgtat gtggacctac cgctctgatg agtgtggtta cacgggcggg
       61 gcagtggcgg atgagttcga caaccccacc acggatatcc gtaaggacag atgcagcaag
      121 tgcatgcgcg ggtgtgagat gcgcagcatg gtggctaatt ttggcggttt cctttccatt
      181 aataaacttt cgcagtaaat cccggtttat gacacagact gaatcagcga ttctggcgca
      241 tgcccggcgg tgtgcgcctg cggagtcgtg cggcttcgtg ataagcacgc cggaggggga
      301 gtggtatatc ccttgtgtga atatttctgc agagccggag gcgtattttc gtatcgcacc
      361 ggaagactgg ctgcgggcag agatgcaggg ggagattgtg gcactggtcc acagtcatcc
      421 cggtgggctg ccctggctga gcgaggctga ccggcggctg cagataaaaa gcgcactgcc
      481 ctggtggctg gtctgccggg gtgacattca caaattccgc tgtgtgccac atctgacagg
      541 acggcgcttt gagcacgggg tgacggactg ttacacgctg ttccgggatg cttatcatct
      601 ggcgggga
//
LOCUS       DAZFBR010000446          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFBR010000446 DAZFBR010000000
VERSION     DAZFBR010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.452"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..71
                     /locus_tag="ACHJZ2_005463"
     CDS             <1..71
                     /locus_tag="ACHJZ2_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4571321.1"
                     /translation="RAIKAEYQQKLKKLRNSRSEVA"
     gene            68..>608
                     /locus_tag="ACHJZ2_005464"
     CDS             68..>608
                     /locus_tag="ACHJZ2_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO4571322.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGAD"
BASE COUNT          201 a          119 c          153 g          135 t
ORIGIN      
        1 gcagggccat caaggcggag tatcaacaga aacttaaaaa actgcgaaac agcagaagtg
       61 aggttgcatg aatatctacg aaagaattga tggcagcaaa taccgaaata tttgggtagt
      121 tggcgatctg cacggatgct acacgaacct gatgaaaaaa ctggagacga taggattcga
      181 caccaaaaaa gacctgctta tctcggtggg cgatttggtt gatcgcggta cagagaacgt
      241 agaatgcctg gaattaatca cattcccctg gttcagagct gtacgtggaa accatgagca
      301 aatgatgatt gatggcttat cagagcgtgg aaacgtcaat cactggctgc ttaatggcgg
      361 tggctggttc tttaatctcg attacgacaa agaaattctg gctaaagctc ttgcccataa
      421 agcagatgaa cttccgttaa tcatcgaact ggtgagtaaa ggaaaaaaat atgtcatctg
      481 ccacgccgat tatccttgtg ataaatacga gtttggaaag ccagttgatc atcagcaggt
      541 aatctggaac cgcgaacgaa tcagcaactc acaagacggg atcgtgaaag aaatcaaagg
      601 cgcggaca
//
LOCUS       DAZFBR010000447          599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFBR010000447 DAZFBR010000000
VERSION     DAZFBR010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.453"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(1..459)
                     /locus_tag="ACHJZ2_005465"
                     /pseudo
     CDS             complement(1..459)
                     /locus_tag="ACHJZ2_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
BASE COUNT          204 a          111 c           87 g          197 t
ORIGIN      
        1 gaaaaaaaag cattacatac tttaacagtt tactgctaaa gaaatgttgt tttgtagaaa
       61 taatgctatt ttcaataatg cctggcaatg aatactgcca aacttcattt tttcttttac
      121 agcaaatttg agacctaaaa cttctatttg ctgattcaat gttaacgttc tatcttcagg
      181 ttcttgtgct gttaattctc tgattgttac aaccagatca gggtcggtag tgattataat
      241 ctcaaaacta cctgtaagct cgccataagg aacgactatt gtattttctg gtaactcaga
      301 aaaagcatca cttcttccta acgaaacatc attaccaatg ataacaagta gacgcccatt
      361 aagtatttca tactcgcatc cattctatgg tccgctcaac aaacgaatgg cataagattc
      421 caataagtct tgtgataaga atttatcatt attttccatg aataccaccc gctaattcca
      481 taagctctag tttagagtag tgtaacaaga cggaaatttc atgaccaaat tatgatgaga
      541 cagaataaaa aaattataat tttgaggacc attttaaatg cctctatgca taactttat
//
LOCUS       DAZFBR010000448          597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFBR010000448 DAZFBR010000000
VERSION     DAZFBR010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.454"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..513
                     /locus_tag="ACHJZ2_005466"
     CDS             <1..513
                     /locus_tag="ACHJZ2_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO4571323.1"
                     /translation="LKGGWNLYTYPLSPVNGMDPLGLYEFKSKNIDDIGIFALAMCNG
                     ESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYS
                     DDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKECSSYLGTPNNTYLKYNPKAKGN
                     GVTIIRQGSN"
     gene            515..>597
                     /locus_tag="ACHJZ2_005467"
     CDS             515..>597
                     /locus_tag="ACHJZ2_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO4571324.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMSDDNI"
BASE COUNT          227 a           75 c          127 g          168 t
ORIGIN      
        1 ctgaaggggg gatggaacct gtatacatat ccgctgagcc cggtgaatgg catggatcca
       61 ttaggattat atgaatttaa atcaaaaaat atagatgata ttggaatatt tgcattggca
      121 atgtgtaatg gagaatcaat taacgagaat aaagaatatg gtggactaat atgtaagaag
      181 caaggtgaat atttccccat gaatccgata agttcaaatg ataatgatag tgtagacttg
      241 cgaaatataa aatgccctga aggttcagag agagtaggcg attatcacac tcacggtttt
      301 tactctgacg ataaaggaaa taaagtaaca aaagaaaatg atgtttatga tagtctaaat
      361 ttttcaagca aagatttaac gaattcttat atgaatggaa tgggaaaaaa agaatgcagt
      421 agttacttgg gaacaccaaa taacacctat ctaaaatata atcccaaagc taaagggaat
      481 ggagttacaa ttatcaggca agggagtaat taaaatgaag gcaatacaat gttgttattt
      541 ccttgcttta gtctccagtc atttaatggc tggagttatg agtgatgaca atatatt
//
LOCUS       DAZFBR010000449          594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFBR010000449 DAZFBR010000000
VERSION     DAZFBR010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.455"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            81..329
                     /locus_tag="ACHJZ2_005468"
     CDS             81..329
                     /locus_tag="ACHJZ2_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4571325.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..>594
                     /locus_tag="ACHJZ2_005469"
     CDS             307..>594
                     /locus_tag="ACHJZ2_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4571326.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQ"
BASE COUNT          183 a          138 c          141 g          132 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acag
//
LOCUS       DAZFBR010000450          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFBR010000450 DAZFBR010000000
VERSION     DAZFBR010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.456"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..75
                     /locus_tag="ACHJZ2_005470"
     CDS             <1..75
                     /locus_tag="ACHJZ2_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571327.1"
                     /translation="ERILDTPEWKDYRPKYSGSTYKYS"
     gene            136..411
                     /locus_tag="ACHJZ2_005471"
     CDS             136..411
                     /locus_tag="ACHJZ2_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO4571328.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(491..>593)
                     /locus_tag="ACHJZ2_005472"
     CDS             complement(491..>593)
                     /locus_tag="ACHJZ2_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HFO4571329.1"
                     /translation="FTTLHDYNHFCDFIEFKHKNIIMNTSQYTGSSW"
BASE COUNT          240 a          127 c           92 g          134 t
ORIGIN      
        1 gaaagaatat tagatacacc agaatggaaa gactatcgtc caaaatactc tggttccaca
       61 tataaatatt cttaatgata ccaacttata acggaatagc ataaaaacac ttttcatgga
      121 gcaaaggaga aaacaatgcc attttcaatc aaaaacagat tttcaagttc acaagtacat
      181 tacccggaaa tatccggtcc cataaaagac aagccagcgt caaagaactg catacttaca
      241 tcaacaacat gtaatgtaga tagctataca gtgtaccaaa aaaaagcctg tagttttgac
      301 atgcgcccac ccggcgcagg agaaagaacc ccaaaactaa aactctcagt tactgagatg
      361 acatggctat ctaaaactat agaaacagag atacacaaca caaaagaata gcaaccactt
      421 accagagaac acaaaaagcc acagacccga aacacctgac tgcaagcaac cacctccaca
      481 ggaggtggtt ttaccatgaa cttcctgtgt actgacttgt gttcataata atatttttgt
      541 gtttaaactc aataaagtca caaaaatgat tgtaatcatg caatgtagta aaa
//
LOCUS       DAZFBR010000451          592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFBR010000451 DAZFBR010000000
VERSION     DAZFBR010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.457"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>592)
                     /locus_tag="ACHJZ2_005473"
     CDS             complement(<1..>592)
                     /locus_tag="ACHJZ2_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4571330.1"
                     /translation="RYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQP
                     SDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKK
                     LEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIENTPAGMQSQV
                     LLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKR"
BASE COUNT          134 a          168 c          126 g          164 t
ORIGIN      
        1 cgtttcagga acacgtcgtt catgaatatc tgatcgccct gaccaacaaa catcggcttt
       61 gtgttgccat tcgcaggatt aatcatcgca atcctgtctg ccgccagcag cacctgactc
      121 tgcattcctg ctggcgtatt ctcaataccg gcaccgatac ccgcaatata aaggcgtccg
      181 tccttcatct gttgcagctt cacagcccac atgctgttca gattattatt tgtatcaacc
      241 tgaaccttct gtatctgctg gatcgctgca ctctggtctt ccagtttctt attgacggtc
      301 tgcgtgattt cattactgac atccgtgatg gacgttctga tttccgccag gtcaggcgca
      361 agctgaccgt tatcaatctg agtccacaac tcctgagcca gatgggtttt ccctatctct
      421 cctttgaaaa aatccagata gccggatgca tcatcactcg gctggccaac agcctccaca
      481 aatgccgatt tgccaacggt gttcacactg cggatataaa agtaataatc atggcccggt
      541 ttgatattga tactggcggc tatccagtac agccccgtgc caaggtagcg gg
//
LOCUS       DAZFBR010000452          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFBR010000452 DAZFBR010000000
VERSION     DAZFBR010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.458"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            75..150
                     /locus_tag="ACHJZ2_005474"
     tRNA            75..150
                     /locus_tag="ACHJZ2_005474"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:109..111,aa:Glu,seq:ttc)
     gene            344..>585
                     /locus_tag="ACHJZ2_005475"
     rRNA            344..>585
                     /locus_tag="ACHJZ2_005475"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          169 a          129 c          150 g          137 t
ORIGIN      
        1 cgtactttgc agtgctcaca cagattgtct gatagaaagt gaaaagcaag gcgtcttgcg
       61 aagcagactg atacgtcccc ttcgtctaga ggcccaggac accgcccttt cacggcggta
      121 acaggggttc gaatccccta ggggacgcca cttgctggtt tgtgagtgaa agtcgccgac
      181 cttaatatct caaaactcat cttcgggtga tgtttgagat atttgctctt taaaaatctg
      241 gatcaagctg aaaattgaaa cactgaacaa cgagagttgt tcgtgagtct ctcaaatttt
      301 cgcaacacga tgatgaatcg aaagaaacat cttcgggttg tgaggttaag cgactaagcg
      361 tacacggtgg atgccctggc agtcagaggc gatgaaggac gtgctaatct gcgataagcg
      421 tcggtaaggt gatatgaacc gttataaccg gcgatttccg aatggggaaa cccagtgtga
      481 ttcgtcacac tatcattaac tgaatccata ggttaatgag gcgaaccggg ggaactgaaa
      541 catctaagta ccccgaggaa aagaaatcaa ccgagattcc cccag
//
LOCUS       DAZFBR010000453          583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFBR010000453 DAZFBR010000000
VERSION     DAZFBR010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.459"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..309
                     /locus_tag="ACHJZ2_005476"
     CDS             <1..309
                     /locus_tag="ACHJZ2_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO4571331.1"
                     /translation="ETDDFDIKKSESEIYDENDEQMINSFEELPVKIEFVLGKKIMNL
                     YEIDELCAKRIISLLPESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGHNNVK
                     "
     gene            299..>583
                     /locus_tag="ACHJZ2_005477"
     CDS             299..>583
                     /locus_tag="ACHJZ2_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4571332.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETG"
BASE COUNT          189 a           76 c          129 g          189 t
ORIGIN      
        1 gaaacagatg actttgatat caaaaaaagc gagagtgaga tttatgatga aaatgacgaa
       61 cagatgatta atagttttga agagttgcct gtaaaaattg agtttgttct tggtaagaaa
      121 ataatgaatc tatatgaaat agacgagctc tgtgcaaaaa gaataatatc tctgcttcct
      181 gaatctgaga aaaatataga gatacgtgta aatggcgcgc taactggcta tggcgaactt
      241 gttgaagtgg atgataaatt gggcgtagag attcattctt ggttatcagg gcataataat
      301 gtcaaatagc atttcgctga tagcgatact atcattattt acactattgc cgtttataat
      361 agcatccggg acatgtttta ttaaattctc gattgttttt gttattgtcc gaaacgcatt
      421 gggacttcag caagtgccat caaatatgac acttaatggg gttgctttgc tattgtcaat
      481 gtttgtaatg atgccggttg ggaaggaaat atattataac agtcagaatg agaatctttc
      541 attcaataat gtagcgtcgg ttgttaattt tgttgagaca ggc
//
LOCUS       DAZFBR010000454          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFBR010000454 DAZFBR010000000
VERSION     DAZFBR010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.460"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..349)
                     /locus_tag="ACHJZ2_005478"
     CDS             complement(<1..349)
                     /locus_tag="ACHJZ2_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571333.1"
                     /translation="MNKNLLTRVIIISDDLYYLQGLSAKLGHHYRFFIECFFCSQNAC
                     NNDLSTLRFDNRFTKNVLLVAVDDVSILAKLPDLARFLMIVSLRKMKNRNIFFSLSEQ
                     TFISRYIKLNKLIT"
BASE COUNT          201 a           97 c           98 g          185 t
ORIGIN      
        1 aagtaatgag tttattcaat tttatatatc ggctaatgaa tgtttgctct gaaagtgaaa
       61 agaagatatt tctgtttttc atctttctta gtgaaacaat cattaaaaac ctggccaaat
      121 ctggcagttt tgcaagtata cttacgtcat ccactgcgac aagaagtacg ttttttgtaa
      181 agcggttgtc aaatctaagt gtacttaggt cattattgca tgcattttga gaacaaaaaa
      241 aacactcaat aaaaaaacga taatggtgac ctagttttgc acttaatccc tgaaggtaat
      301 atagatcatc cgaaatgata attacccttg ttagtaagtt cttgttcatt gcactaactc
      361 aattgcttgc agcaaaacag cccatcttca tggagtaaaa attttgaaag tgaataaagc
      421 tttttactaa agatgtaaca atgggcattc catatgcgac ataacaactg cagtaacaca
      481 actaatcttt gcgtgttctt tatattagag agtaacagcg tgatatctgt atctctaaaa
      541 gaatagcgat gtcctgcaat cgatgagcac aatatatgac a
//
LOCUS       DAZFBR010000455          580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFBR010000455 DAZFBR010000000
VERSION     DAZFBR010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.461"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            72..>580
                     /locus_tag="ACHJZ2_005479"
     CDS             72..>580
                     /locus_tag="ACHJZ2_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571334.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVLLKE"
BASE COUNT          196 a          110 c          112 g          162 t
ORIGIN      
        1 ttcacacgaa ttgtatctat tttgaacgaa atattttata gagtgtggaa ttttaagaaa
       61 ggacatctga aatgttaaac cgtgacttcg atcatctctc aacgctatta catctctttt
      121 ttgaacgtga ggatatttgc caaaagctta atattattga ccaaaacaat atcaccggtt
      181 gggaagtgtg gttccaggta gagtttgcta atatgctctg ctcaacagac catgaatggt
      241 ggcgtgaaca agcactttcc tgtgatatgc gcaaaaaacc tgagcgcccg acactacgca
      301 cagatttctt actacgtaaa aaaggctggg cacaagatag ctatatcgcg cttgaaataa
      361 aacaaaacag agacgcgacc agttgtgtta aaaatatgat cgcagactta gaaaaaagcg
      421 caaaaataaa acgttccgaa ctcgatttac gcagtttctg gaccttgggt atcaccccta
      481 tgatagacag ggaaaggctg gactctctca tcgattgcta tttagatgag aaattttatc
      541 ttactaaatc tcgtaaaaaa cacgttttac ttaaagaaat
//
LOCUS       DAZFBR010000456          575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFBR010000456 DAZFBR010000000
VERSION     DAZFBR010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.462"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>575
                     /locus_tag="ACHJZ2_005480"
     CDS             <1..>575
                     /locus_tag="ACHJZ2_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571335.1"
                     /translation="DALAQKIREGWQPYGGPFSSYTDDGAALIQAIVAEGDVSTPVVV
                     KLTGGEGAVISATRDPEYYFIVVLAGQSNSMAYGEGLPLPETYDRPDPRIKQLARRST
                     VTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGA"
BASE COUNT          130 a          139 c          182 g          124 t
ORIGIN      
        1 gatgcacttg cgcaaaaaat tcgtgaagga tggcaaccat acggtgggcc gttttcttcg
       61 tatacggatg atggcgcagc acttattcag gcgattgtcg cagaaggtga tgtgagcaca
      121 cctgttgtgg tgaagctgac aggtggagaa ggtgcagtaa tcagcgccac cagagacccg
      181 gagtattact ttattgtggt tctggcgggg cagtcaaaca gcatggcata tggtgaaggc
      241 cttccgctgc cggagacata tgaccgtccg gacccgcgta ttaagcagct ggcgcgccgc
      301 agtacggtga caccgggcgg tgtcgcctgt aaatataacg acatcattcc ggcggaccat
      361 tgtctgcatg atgtgcagga catgagccgc cttaaccatc cgaaagcgga cctgtcaaag
      421 gggcagtacg gaaccgtggg gcaggggctg catatcgcca aaaaattgct gccgtttata
      481 ccggcgaatg cgggcattct gctggttccg tgctgtcgtg gtggttcagc gttcaccacc
      541 ggagctgatg gcacatacag tgacgcgagt ggtgc
//
LOCUS       DAZFBR010000457          572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFBR010000457 DAZFBR010000000
VERSION     DAZFBR010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.463"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>572
                     /locus_tag="ACHJZ2_005481"
     CDS             <1..>572
                     /locus_tag="ACHJZ2_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4571336.1"
                     /translation="EAMSLFADVMNARLEQLDKAATPVEKIWDDVKTWTSDAWAWIGD
                     HTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETTKNVPGVGALFGELAEE
                     NRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRATGVSKGPGQQEAVSRLAEE
                     LTGKKHTSPKTRSAGEREEEQAREALLALEA"
BASE COUNT          150 a          125 c          189 g          108 t
ORIGIN      
        1 gaggcgatgt ccctgtttgc ggatgtcatg aatgcacgtc tggagcaact tgataaagcg
       61 gccacgccgg tggaaaaaat ctgggacgat gttaaaacct ggacttctga cgcatgggca
      121 tggataggtg atcatacact gggggcactc agtctgatca ctgacgtggt ggccggaacc
      181 gttgaacaag tgaagctgct gcttgtgcag ggggatctgg cgctggctga atttattcag
      241 tcagcctggg aaacgacaaa gaatgtgccc ggcgttggtg cgttgtttgg tgaactggca
      301 gaagagaacc gcgtatttat tgagaaaaca aaacgcgatg aactggcgct gagaaaatcc
      361 attgcggaac gggatgcgcg tatacgccag ggggaaatgg ggtacatcaa ccgctcgcgt
      421 gcaacaggcg tcagcaaagg tccggggcag caggaagccg tcagccgtct ggctgaagag
      481 ctgacaggta aaaagcatac atcaccgaaa acgcgctctg ccggggagag ggaagaggag
      541 caggcaagag aggctctgct tgcccttgaa gc
//
LOCUS       DAZFBR010000458          566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFBR010000458 DAZFBR010000000
VERSION     DAZFBR010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.464"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>566)
                     /locus_tag="ACHJZ2_005482"
     CDS             complement(<1..>566)
                     /locus_tag="ACHJZ2_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571337.1"
                     /translation="SSGNPPTFSLTPDGRLTAKNADISGSVNANSGTLNNVTINENCQ
                     IKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQVMIPPVLFRGGK
                     HENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTLTLTFT
                     VSSSGANNWTPTTSISDLLVVVVKKSTAG"
BASE COUNT          123 a          146 c          138 g          159 t
ORIGIN      
        1 cctgctgtgg attttttcac caccacaacc agcagatcgc tgatactggt tgttggtgtc
       61 cagttattcg ctcctgatga agatacggtg aatgtcagtg tcagcgtccc ctgtccggca
      121 ggcatatcta taactgagga aaatacgccc tgagcatccg tcgtggactg attaaaaatc
      181 tcctgaccat tgcgggtcac tcttaaccgg caggttgaat accagtatga ctgttggtta
      241 ttactgttga aattctcatg cttaccaccg cggaataaca ctggcggtat catgacctgc
      301 cggtcaaact tctgatcatc actgattctt accgtgatgg tgccactggc ataagtgctc
      361 gtgcggggga aagacttgct gaccgttttg acaatatcgc cttcaatctg gttggctgac
      421 agtttcccct taatctgaca gttctcatta atcgtgacgt tgttgagcgt ccctgagttc
      481 gcattcacac tgccactgat atccgcattt ttcgccgtca gtcgcccgtc cggcgtcagg
      541 gaaaacgtcg ggggattgcc ggacga
//
LOCUS       DAZFBR010000459          562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFBR010000459 DAZFBR010000000
VERSION     DAZFBR010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.465"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>562
                     /locus_tag="ACHJZ2_005483"
     CDS             <1..>562
                     /locus_tag="ACHJZ2_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO4571338.1"
                     /translation="DSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVG
                     EEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGT
                     IKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDN
                     IKMVVTLIHPIAMDDGLRFAIREGGRT"
BASE COUNT          136 a          140 c          155 g          131 t
ORIGIN      
        1 tggattccta cattccggaa ccagagcgtg cgattgacaa gccgttcctg ctgccgatcg
       61 aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta gaacgcggta
      121 tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag aagtctacct
      181 gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg ccgtgctggt gagaacgtag
      241 gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc
      301 cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg tccaaagatg
      361 aaggcggccg tcatactccg ttcttcaaag gctaccgtcc gcagttctac ttccgtacta
      421 ctgacgtgac tggtaccatc gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca
      481 acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg
      541 caatccgtga aggcggccgt ac
//
LOCUS       DAZFBR010000460          559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFBR010000460 DAZFBR010000000
VERSION     DAZFBR010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.466"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..244)
                     /locus_tag="ACHJZ2_005484"
     CDS             complement(<1..244)
                     /locus_tag="ACHJZ2_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571339.1"
                     /translation="MDNLIGTPPNHAVPHNYIDMEQMEYLCHLNRFSKLSNDFLINGP
                     REDLFTYKYVLDGSFSNLQHLLPKGQLQKIQQRLSSL"
BASE COUNT          195 a           93 c           92 g          179 t
ORIGIN      
        1 taagagaact taatctctgt tgtatcttct gtagttgtcc tttaggaagt aaatgctgta
       61 aatttgaaaa tgaaccatct aaaacatatt tatatgtaaa aaggtcctct cttggaccat
      121 ttattaaaaa atcgttagac aatttagaaa aacgattgag atgacataaa tattccattt
      181 gctccatgtc tatatagtta tggggaactg cgtggtttgg tggtgtgcca ataagattat
      241 ccataaatca ccttgataaa atttaataaa atctctttat tttaacctcg acaataaaag
      301 cggctataaa aaaacgctca ttttctgatg ctgcggttaa cccgtggaat gtaccgttaa
      361 cccccaaaaa attgcaaatc cagggtgaac accctgggtt aaaatggaaa taatgagcta
      421 ttttttacag tattcaccgg taatataagt aacacttgtt gctaacctaa ctagcagtac
      481 agttaattaa caacatagaa gttattgttt ttcaaacaac tcaatattac acgccggagg
      541 atttatggtc gctaaatta
//
LOCUS       DAZFBR010000461          557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFBR010000461 DAZFBR010000000
VERSION     DAZFBR010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.467"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(31..402)
                     /gene="grlR"
                     /locus_tag="ACHJZ2_005485"
     CDS             complement(31..402)
                     /gene="grlR"
                     /locus_tag="ACHJZ2_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO4571340.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVL
                     SEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVLSNDNNLTLHCHVRGNEKLFVDV
                     YAKFIEPLVIKNTGMPQVYLK"
BASE COUNT          182 a          109 c           70 g          196 t
ORIGIN      
        1 aataaatcga cataaaaaac atacataaaa ttattttaaa taaacttgtg gcattcctgt
       61 gtttttaata actaatggtt ctataaattt ggcataaaca tcaacaaaca atttttcatt
      121 tcctcttaca tggcaatgta atgtgagatt attatcatta ctcagtactt ttttagggat
      181 aactaattga taatcttgtt caatgtttag caccgaggga atttcgtaat tatatcgatg
      241 gacatgaagt atgatgtcct catcttcaga gaggaccccc tgatacacag aagcaatatc
      301 gccgccagtg atcatatttc catttttaat cagtatacct tccccacagg agtcttcatt
      361 gctaataaat ataatgctat agatgccatc cttcataatc ataactcact cagtttcata
      421 ctaacctcac tcctttcaat ttgttctatc cataagcaat atcaagaata atggagacct
      481 ttctttttct ccagattgca atgtaatata tcattaaata tactcatgtt tcaacctgct
      541 gtttccctgg ttaacat
//
LOCUS       DAZFBR010000462          553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFBR010000462 DAZFBR010000000
VERSION     DAZFBR010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.468"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..172)
                     /locus_tag="ACHJZ2_005486"
     CDS             complement(<1..172)
                     /locus_tag="ACHJZ2_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4571341.1"
                     /translation="MLHDHLAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSP"
BASE COUNT          181 a          135 c           70 g          167 t
ORIGIN      
        1 tcggtgaaac cggagtccgg cgcgccttac gcaaacactc tgctcgtttc tgtgccgcca
       61 cttttctttt ctggtcatca cttagctgta ccatcacttt tgcccatcgt tcagctgctc
      121 tccggtacag tccttttttc tccagacatt ctgccaggtg atcatgtagc ataagtgacc
      181 tccgattatc tacagactgc tatcctgaat ttaccttcct ttaatgaaat aacaataaaa
      241 aataaatcac agaaaaacaa caaaacaaca cacaaaaaaa ccaaataata aacccaaata
      301 atcaccttat tttattattt tttgagagag ctattactga acaaaaaacg ctgactatat
      361 actcaaaacc aaacaactat tctgccaatc aggtataatg gcaacacaag gaattaccgt
      421 gtttttgcct tctctgccca tacaatacgg acatatactt catactctat tgtaatattt
      481 ccatccatgc gaacaacttc atttatctgt aaataatatt caaaatattt atcacagaaa
      541 tcgtttttgg cca
//
LOCUS       DAZFBR010000463          545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFBR010000463 DAZFBR010000000
VERSION     DAZFBR010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.469"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>545)
                     /locus_tag="ACHJZ2_005487"
     CDS             complement(<1..>545)
                     /locus_tag="ACHJZ2_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4571342.1"
                     /translation="ELLDYQFSTNEIAAVVAERDVEWWQNRASVLTTPQLASGYFNAG
                     FLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRY
                     SINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLK
                     PVNSNQYRYCAKHKFKQKHYM"
BASE COUNT          167 a          108 c           85 g          185 t
ORIGIN      
        1 ccatataatg cttttgttta aatttatgtt ttgcacaata ccgatactga ttgctattta
       61 caggtttaag taaatcttct ttactccacg gagaagctgc tttggcgatc aaaaaactac
      121 gtgacaccgg atagtcagcc cactcatgcc aaggttttgt aggtcccaca tagtgtataa
      181 acacggtgtc atcattgact ggattatcaa ctttgtcttt taattcatag ttaatactat
      241 atcgggtatt atatttctcc gaaataaact tcactttacc attcaataat acattcagta
      301 catcttgatc aaggtgggtg attttactta cccaatctgg gtcacgcaac atttcaatag
      361 cttttgacga aatgttattt agattccact catcaatatt tatcagtaaa aaaccagcat
      421 tgaaatatcc agaagctaac tgtggtgtag ttaataccga ggctcgattt tgccaccatt
      481 caacatctct ttcagctaca actgcggcaa tttcattagt agaaaattga taatctaaga
      541 gttct
//
LOCUS       DAZFBR010000464          531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFBR010000464 DAZFBR010000000
VERSION     DAZFBR010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.470"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
BASE COUNT          152 a           90 c          122 g          167 t
ORIGIN      
        1 gtctaaacaa gggaagcttt gcaagctaac tcagtgagct tggtgtaaat cagtgtttac
       61 ccgccatcag gctgagcata attctcatca tgaaatatgt ttcctggttt gtggcttgta
      121 actggtcact tctgaagtcg atctggagag gcttattgat gttggtgttt tcaggatgat
      181 gtttcactta gtttgtttgc cgtatcgccc ggcgaatggc tgtgattgag gaaggtttaa
      241 gtcgtagtga ccaaagctat atttaccaac gaatgtagat gaaaaaatca tctcctgcgt
      301 tcccccatat ctccaggata aaaaggaatg taacaatctc atggcgtaag ctgacgaatc
      361 agccaggaat aatcgctagg gacctaagaa ttagcttgat aatagccact aagaaattac
      421 tgtgttccat gaaatagcca ttttgtggca aatggagttg actaataatg tcatatgtga
      481 gacggctagt tgaacgaata ttaaattttg ctgaattttt tatgttgatt t
//
LOCUS       DAZFBR010000465          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFBR010000465 DAZFBR010000000
VERSION     DAZFBR010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.471"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>530
                     /locus_tag="ACHJZ2_005488"
     rRNA            <1..>530
                     /locus_tag="ACHJZ2_005488"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          143 a          120 c          167 g          100 t
ORIGIN      
        1 agacccgaaa cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga
       61 ggaccgaacc gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc
      121 aatcaaaccg ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt
      181 catctccggg ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg
      241 caaactgcga ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt
      301 cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga
      361 aacgatgtgg gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag
      421 aaagcgtaat agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca
      481 tgcaccgaag ctgcggcagc gacactgtgt gttgttgggt aggggagcgt
//
LOCUS       DAZFBR010000466          511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFBR010000466 DAZFBR010000000
VERSION     DAZFBR010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.472"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..85
                     /locus_tag="ACHJZ2_005489"
     CDS             <1..85
                     /locus_tag="ACHJZ2_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571343.1"
                     /translation="LKENTETVEMNSKVWGRLKKIITFFKK"
     gene            complement(161..463)
                     /gene="bamE"
                     /locus_tag="ACHJZ2_005490"
     CDS             complement(161..463)
                     /gene="bamE"
                     /locus_tag="ACHJZ2_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO4571344.1"
                     /translation="MKNETQESVKTKIVKGKTTKQDVLASFGEPDSRSLIDGEEQWSY
                     TMYNSQSKATSFIPVVGLLAGGADSQTKSLTVSFKGEKVSTYIFNAGTSNVKTGIF"
BASE COUNT          142 a           97 c          109 g          163 t
ORIGIN      
        1 tttaaaggaa aatactgaga ctgtagaaat gaacagtaaa gtttggggga ggctgaaaaa
       61 gataatcact ttctttaaaa agtgatttat gtttctgtaa aaagcccgta ggtatcaact
      121 gcgggctaaa ttttcccttg atgctggttt tgtcttttat ctaaaaaatg ccagtcttca
      181 cgttgcttgt tccagcatta aagatgtatg tgctgacttt ttcgcctttg aaagaaactg
      241 tcagagattt agtttgtgag tctgcgccac ctgcaagcag tcccacaacg gggatgaaag
      301 aggttgcttt ggactggctg ttatacatag tgtatgacca ttgttcttca ccatcgatca
      361 aagaacggct gtcaggttca ccgaacgatg ctaacacgtc ctgttttgta gttttgcctt
      421 taacaatttt ggttttcaca ctttcctgcg tctcattttt caatgactgg ttgcctactg
      481 aagaacaacc tgccaaggtc agaaaaacga a
//
LOCUS       DAZFBR010000467          507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFBR010000467 DAZFBR010000000
VERSION     DAZFBR010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.473"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..131
                     /locus_tag="ACHJZ2_005491"
     CDS             <1..131
                     /locus_tag="ACHJZ2_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4571345.1"
                     /translation="EQARVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            259..507
                     /locus_tag="ACHJZ2_005492"
                     /pseudo
     CDS             259..507
                     /locus_tag="ACHJZ2_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
BASE COUNT          151 a           80 c          142 g          134 t
ORIGIN      
        1 cggagcaggc cagggtaatc acgcaggtgg tgatactgga tgcggataaa aagcagatac
       61 agtgcgtggt aagaccgctg caaatcctgc gtgctgacgg gacgtgggaa aatattggcg
      121 ggatgaagta acccgacagc ttcacaaaac cggagtccgg ctccggtttt tgttgtcatg
      181 tacggtggat gtttgttatg actccctgtg tttggaatga atatttaata acaggtgtct
      241 ggaaatatag gggcaaatct actggatagg ctattggggc gtgagaatcg aatggagaga
      301 agggctgtgg ctctggaaag gcaattaaat ggaggtgtcg attttttaag gagtgttaat
      361 aactattttc agagtgtcat ggcagaacac agagaaaata aaacaagtaa taaaatatta
      421 atggaaaaaa taaattcctg tgtatttgga acggattcta atcacttttc ttgcccggag
      481 tcatttttga catgcccgat aacgctg
//
LOCUS       DAZFBR010000468          504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFBR010000468 DAZFBR010000000
VERSION     DAZFBR010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.474"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..302
                     /locus_tag="ACHJZ2_005493"
     CDS             <1..302
                     /locus_tag="ACHJZ2_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO4571346.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..>504
                     /locus_tag="ACHJZ2_005494"
     CDS             299..>504
                     /locus_tag="ACHJZ2_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO4571347.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASTDLSEAAVRLEALW"
BASE COUNT          114 a          116 c          170 g          104 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtacacag atcagcatgg tttcaggggc
      421 cagcattggc gcattcaatg gtgccattat cgcggcctct accgatctgt cagaagctgc
      481 cgtacgcctg gaggcgctct ggga
//
LOCUS       DAZFBR010000469          504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFBR010000469 DAZFBR010000000
VERSION     DAZFBR010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.475"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>504
                     /locus_tag="ACHJZ2_005495"
     CDS             <1..>504
                     /locus_tag="ACHJZ2_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4571348.1"
                     /translation="GDAYANPLSDDDWQRLAKVKDLTPGEMTAEPYDDNYLDDEDADW
                     TATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWVS
                     SIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPATAIKVTPTSGTVAKGKTTTLTV
                     SFEPESAT"
BASE COUNT          135 a          115 c          165 g           89 t
ORIGIN      
        1 gggtgatgct tatgcaaacc cgttgtcaga cgatgactgg cagcgactgg ctaaggtgaa
       61 ggatctgacg ccgggcgaga tgacggcaga accctacgat gataactacc tggatgatga
      121 agacgcggac tggaccgcga ccgggcaggg gcagaagtct gcaggagata ccagttttac
      181 gctggcctgg aaaccgggag aagaaggtca gaaagggctt ataggctggt ttgaaagcgg
      241 ggatgtgcgg gcctataaaa tccgtttccc aaatggcacg gtggatgtgt tccgtggctg
      301 ggtcagcagt atcggtaagg ccgtgacggc gaaagaagtg atcacccgca cggtgaaagt
      361 cactaacgtg ggcaaacctt ccgtggcgga agaacgcagc gaaattacgc cggccactgc
      421 aattaaggtg acaccgacat ccggtacggt ggcaaaaggg aaaacaacca ccctgacggt
      481 ttcttttgag ccggaaagtg caac
//
LOCUS       DAZFBR010000470          504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFBR010000470 DAZFBR010000000
VERSION     DAZFBR010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.476"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..67
                     /locus_tag="ACHJZ2_005496"
     CDS             <1..67
                     /locus_tag="ACHJZ2_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571349.1"
                     /translation="NGLQKNQKNFVCLLVMRRSIQ"
     gene            64..>504
                     /locus_tag="ACHJZ2_005497"
     CDS             64..>504
                     /locus_tag="ACHJZ2_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO4571350.1"
                     /translation="MTHKDITNLIAGDRQSFLKLTLWEFILDLFPMYHIKEAKETIVE
                     FVTKTEDKVKRFERIKSLAIPEEKWRFSTTTEFTVDENKDIIVSRVFKININSKDNHN
                     NAEENTMRSVLSEERNYNSYKHDIHFDEKAFQSGILKMQFPNDNE"
BASE COUNT          200 a           62 c           88 g          154 t
ORIGIN      
        1 taatggattg caaaaaaatc agaaaaattt tgtttgtctg ttggtaatga ggagaagtat
       61 tcaataaccc ataaagatat aacaaacctt atagctggtg atagacaatc ttttcttaaa
      121 ctaactcttt gggaatttat actcgatctc tttcccatgt atcatattaa ggaggctaaa
      181 gaaacaattg tagaatttgt aacgaaaact gaggacaaag ttaagagatt tgagagaatt
      241 aaatctctcg ctattccaga agaaaaatgg cgctttagta cgacgacaga atttacagta
      301 gatgagaata aagacattat tgttagtaga gtatttaaaa taaatattaa ctcgaaagat
      361 aatcataata atgctgaaga aaatacaatg cgttctgtat tatcagaaga acgcaattat
      421 aatagttata aacatgatat tcattttgat gagaaggctt ttcaatcagg gatattgaaa
      481 atgcagttcc ctaatgataa tgaa
//
LOCUS       DAZFBR010000471          493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFBR010000471 DAZFBR010000000
VERSION     DAZFBR010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.477"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..492)
                     /locus_tag="ACHJZ2_005498"
     CDS             complement(<1..492)
                     /locus_tag="ACHJZ2_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFO4571351.1"
                     /translation="MNYPIMMVHDEWLGDLTGVNQVSFRRLRETSSTRNQLNKILRQE
                     IHDKISGVELSDINKEGFLYQSIGKIRLLALSSALFDIQCPDYIFSRLYRETLIREIG
                     YQNVKQLSFYWQGGQCKPEYGEERFCAELIKYGAGNLEWLFADNPLWTIVKYLLPKSG
                     EIKP"
BASE COUNT          156 a          106 c           66 g          165 t
ORIGIN      
        1 tggttttatt tctccacttt tcggcaaaag atatttgact atggtccata aaggattatc
       61 agcaaatagc cactccaaat ttcctgcacc gtattttatt aactcagcac aaaaccgttc
      121 ttcaccatat tctggtttac attgccctcc ctgccaatag aatgataact gcttcacatt
      181 ttgatatcct atctctctaa tcagagtttc tcgatataat cgtgagaaaa tataatcagg
      241 gcattgaatg tcaaataatg cactacttag agcaagcaat cttattttcc caattgactg
      301 atataaaaaa ccttccttat ttatgtcaga taattccaca ccagaaattt tatcatgtat
      361 ttcctgacgc aatatcttat tgagttgatt acgagtagaa ctagtttctc ttaaacgccg
      421 aaaactgacc tgattgacac cggttaaatc acctagccat tcatcatgaa ccatcatgat
      481 tggataatta att
//
LOCUS       DAZFBR010000472          487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFBR010000472 DAZFBR010000000
VERSION     DAZFBR010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.478"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(87..353)
                     /locus_tag="ACHJZ2_005499"
     CDS             complement(87..353)
                     /locus_tag="ACHJZ2_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO4571352.1"
                     /translation="MIITIALISLLSACTRENSLKENQNKRSGESQAIVDARQFFSEH
                     PEYISSAELEEKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
     gene            complement(383..>487)
                     /locus_tag="ACHJZ2_005500"
     CDS             complement(383..>487)
                     /locus_tag="ACHJZ2_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO4571353.1"
                     /translation="ALNQLNIKGTVELFIALCEWDKHYFKLQSCVRES"
BASE COUNT          154 a           84 c           78 g          171 t
ORIGIN      
        1 ataatgacaa taatttaaaa aattaaacat gtatcgtagt caaaaattta taagaatcta
       61 cacgcaaaaa acgtgtagat ttttatttaa tgagttaaac gatcttgcga aatgacaagc
      121 aattgataca tactcatctc tttataagct ggaactgtag taacctgtgc aaattcgcga
      181 tatagctttt cctccagttc tgctgatgat atatattccg ggtgttcgct aaagaactgt
      241 cttgcatcta ctatcgcttg ggattcgcca cttcttttat tttgattctc ctttaatgaa
      301 ttttctctgg tgcatgcaga gagcaacgat atcagcgcga tggttattat gattttacta
      361 ttatatcatt actaatgaca tatcaacttt ctcttacgca agattggagt ttaaaataat
      421 gtttatccca ttcacagagt gctataaaca actcaacagt accttttatg ttaagttgat
      481 tgagggc
//
LOCUS       DAZFBR010000473          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFBR010000473 DAZFBR010000000
VERSION     DAZFBR010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.479"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>473
                     /locus_tag="ACHJZ2_005501"
     CDS             <1..>473
                     /locus_tag="ACHJZ2_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4571354.1"
                     /translation="HGDYHHSETVIHRYGTGAMVLCWHCDNQLREQTSDSLDQLAQQN
                     LAAWMIDIIRHAMNGAQERELSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSV
                     YRESDIILGEQTATSILKQRTKNIALPSHTHQQQNPPQEKTVVSIAVDPESPESF"
BASE COUNT          122 a          131 c          126 g           94 t
ORIGIN      
        1 acacggtgat tatcatcata gcgaaaccgt cattcaccgt tacggtaccg gcgcgatggt
       61 gttgtgctgg cactgcgaca accagctgcg ggagcagaca tctgattcac tggatcaact
      121 tgctcaacag aatctggccg cctggatgat tgacatcatc cgtcacgcaa tgaatggcgc
      181 acaggagcgt gaattatctc tggctgaatt atcctggtgg gcggtccgca atcaggtggc
      241 ggacgcgcta ccggaagcgg tattacgtcg ttcgctgggg ttgcgtgcgg aaaaaatccg
      301 ctccgtatac cgtgaaagcg acatcatact gggagaacag accgccacca gcatactgaa
      361 gcagcgcaca aaaaatattg cgctaccgtc tcacacccac cagcaacaga acccaccaca
      421 ggaaaagacg gtggtcagca ttgccgttga tccggagtct ccggaatcct tca
//
LOCUS       DAZFBR010000474          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFBR010000474 DAZFBR010000000
VERSION     DAZFBR010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.480"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>472
                     /locus_tag="ACHJZ2_005502"
     rRNA            <1..>472
                     /locus_tag="ACHJZ2_005502"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          113 c          141 g           99 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgagttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg ga
//
LOCUS       DAZFBR010000475          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFBR010000475 DAZFBR010000000
VERSION     DAZFBR010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.481"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
BASE COUNT          164 a          105 c           63 g          139 t
ORIGIN      
        1 acccccggta gagttgcccc tactccggtt ttcgagacct atgctatggg ttaataaaat
       61 caatatatta tgtgttttat ttggaataaa tattctatat ttaggattga aaaatcagat
      121 ggttagcatc aaacaacctc agaatattcc aagcaaacag gttaaaaata aaactgcacc
      181 cgaacaattg ataacgacag aaaacgcctt ttccgagcca ccaaaattac ttcgcatcct
      241 atttattgct cacgttaacg ccttgtataa ctcgagctct ccacggtatt taacctctct
      301 tctgtttaac tataattcca ataaatctcg tcactgagct caatcacttc gaaagtcaag
      361 cacaatatga atttcacata agctatcaat gaaaatcgga ggtcatacca ttaatcaaca
      421 ataaaccaag tctttctcat ataatcactt gacaaacact catgataatt g
//
LOCUS       DAZFBR010000476          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFBR010000476 DAZFBR010000000
VERSION     DAZFBR010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.482"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>469
                     /locus_tag="ACHJZ2_005503"
     CDS             <1..>469
                     /locus_tag="ACHJZ2_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4571355.1"
                     /translation="IVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIW
                     DGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGG
                     TEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYT"
BASE COUNT           99 a          125 c          156 g           89 t
ORIGIN      
        1 attgtggggc tgcaggtgga tgcggagcag tttggcggtc agcagatgac ggtgaactac
       61 catatccgcg gtcgcatcat ccaggtaccg tcaaactatg acccggaaaa acgcacgtac
      121 agcggcatct gggacggcag cctgaaaccg gcatacagca acaaccctgc ctggtgcctg
      181 tgggacatgc tgacccaccc gcgctacgga atgggaaaac gcctgggggc ggcggatgtg
      241 gacaagtggg cgctgtatgc cattgcgcag tactgcgacc agacggtccc ggatggtttc
      301 gggggcacag agccgcggat gactttcaat gcgtacctgt cacaacagcg taaggcgtgg
      361 gacgttctca gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa cgggcagacg
      421 ctgacgtttg tgcaggaccg tccgtcagat gtggtgtggc cctacacca
//
LOCUS       DAZFBR010000477          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFBR010000477 DAZFBR010000000
VERSION     DAZFBR010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.483"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>469
                     /gene="wzx"
                     /locus_tag="ACHJZ2_005504"
     CDS             <1..>469
                     /gene="wzx"
                     /locus_tag="ACHJZ2_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFO4571356.1"
                     /translation="TLKKYDTSSALGREQERYRIISLSVISSLISKILSLLSLILTVS
                     LTLPYLGQERFGVWMTITSLGAALTFLDLGIGNALTNRIAHSFACGKNLKMSRQISGG
                     LTLLAGLSFVITAICYITSGMIDWQLVIKGINENVYAELQHSIKVFVIIFGLG"
BASE COUNT          147 a           67 c           95 g          160 t
ORIGIN      
        1 gcactttaaa aaaatatgat acatcaagtg ctttaggtag agaacaggaa aggtacagga
       61 ttatatcctt gtctgttatt tcaagtttga ttagtaaaat actctcacta ctttctctta
      121 tattaactgt aagtttaact ttaccttatt taggacaaga gagatttggt gtatggatga
      181 ctattaccag tcttggtgct gctctgacat ttttggactt aggtatagga aatgcattaa
      241 caaacaggat cgcacattca tttgcgtgtg gcaaaaattt aaagatgagt cggcaaatta
      301 gtggtgggct cactttgctg gctggattat cgtttgtcat aactgcaata tgctatatta
      361 cttctggcat gattgattgg caactagtaa taaaaggtat aaacgagaat gtgtatgcag
      421 agttacaaca ctcaattaaa gtctttgtaa tcatatttgg acttggaat
//
LOCUS       DAZFBR010000478          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFBR010000478 DAZFBR010000000
VERSION     DAZFBR010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.484"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..355)
                     /locus_tag="ACHJZ2_005505"
     CDS             complement(<1..355)
                     /locus_tag="ACHJZ2_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFO4571357.1"
                     /translation="MPVDLTPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEM
                     LENMDLLSNYQLEELL"
BASE COUNT          157 a          100 c           59 g          152 t
ORIGIN      
        1 ctaacaactc ctctaactgg tagttagaaa gcaaatccat attttcgagc atctcaattt
       61 cacgcagtac tgtaacttca cggcttgtca aactaccatg attaagtgta cgttcaatat
      121 gttctatata ttgaaggcca atctggatat tatcattcga taaaccatcc tggttaagac
      181 ttcccataaa atatccattt acctgcatag gtcgtattga caccatcaac tcacccagtc
      241 ttatttgagc ttctccacga atagtctgat tacgtatctc agacaaggtt tcttgaggaa
      301 tgtcagacaa aaaactaact ccaggtaaaa tataaggcgt taaatctaca ggcattcctc
      361 tccctccatt ttaataactt aattttgctt tagtaaaaaa taaaagtaca taaaaccata
      421 tttatatcat taattatcac ttacaacaaa aagcttctct ttttgtat
//
LOCUS       DAZFBR010000479          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFBR010000479 DAZFBR010000000
VERSION     DAZFBR010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.485"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            72..>464
                     /locus_tag="ACHJZ2_005506"
     CDS             72..>464
                     /locus_tag="ACHJZ2_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFO4571358.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMS
                     HHLGYDKNQPKPGANSRNGYSTKTVITGDGHLELRTPRDRDGSFEPQLVKKNQTRITG
                     MDNQILSLYAKGLTTREIAAAFKELYDAD"
BASE COUNT          136 a          122 c          105 g          101 t
ORIGIN      
        1 agcctgtaca tagatttgtg taattgcctg attttgatat gttcaatcca acatcaaaag
       61 caggttaatt tatggacgaa aaacagttgc aggctctggc taacgaactg gccaaaaatc
      121 tcaaaacccc tgaagatctc agccagttcg atcgcctgct gaagaaaatc agcgttgagg
      181 cagctctcaa cgctgaaatg tcccaccatc tgggctacga taaaaaccag cctaaaccgg
      241 gtgccaactc ccgcaatggc tattccacaa agaccgttat caccggtgat ggccacctgg
      301 aactacgcac tccgcgcgat cgtgatggct cttttgaacc gcaacttgtg aagaaaaacc
      361 agacccggat caccgggatg gataaccaga tcctgtcgtt gtacgccaaa ggcctgacaa
      421 cccgcgaaat agcggctgcg tttaaagagc tgtatgacgc tgat
//
LOCUS       DAZFBR010000480          463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFBR010000480 DAZFBR010000000
VERSION     DAZFBR010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.486"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            3..>463
                     /locus_tag="ACHJZ2_005507"
     CDS             3..>463
                     /locus_tag="ACHJZ2_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571359.1"
                     /translation="MGSNDTDHITIGIDNQEPIKLPASYKDKELVRTIINDNIVEKTH
                     DDINNAEFTDGGIDDRSDIGIDSNDTDDIDIDNQEPIKSPANDENKKVGQTIINENIV
                     ENTHDINNKKMIFSAIKEIYDEDPSFIFDKISHKLRHTVTEFDESGKSEPTG"
BASE COUNT          191 a           79 c           82 g          111 t
ORIGIN      
        1 atattggttc caatgataca gaccatatta ctattggtat cgacaaccaa gaaccaataa
       61 aattacctgc gagttataaa gacaaagaac tcgtccgtac tatcattaat gacaacatag
      121 ttgagaagac tcatgatgat ataaataatg ctgaatttac tgatggtggt attgatgatc
      181 gtagtgatat tggcattgac tccaatgata cagacgatat tgatattgat aatcaagaac
      241 caataaaatc acccgcgaac gatgaaaaca aaaaagtcgg ccaaaccatc atcaatgaaa
      301 acatagtaga gaatactcat gatatcaata acaagaaaat gatcttcagc gcaataaaag
      361 aaatatatga tgaagatcca agttttatct tcgataaaat atcacacaaa ctcagacata
      421 cggtaacgga attcgatgag agcgggaaaa gcgaaccaac ggg
//
LOCUS       DAZFBR010000481          462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFBR010000481 DAZFBR010000000
VERSION     DAZFBR010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.487"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>462)
                     /gene="wzy"
                     /locus_tag="ACHJZ2_005508"
     CDS             complement(<1..>462)
                     /gene="wzy"
                     /locus_tag="ACHJZ2_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O157 family O-antigen polymerase"
                     /protein_id="HFO4571360.1"
                     /translation="PYVVSESIKNDFFPTPELEKELKAIINRIQGIKHQDLFYGERLH
                     KQVFGDMGANFLSVTTYGAELLVFFGFLCVFIIPLGIYIPFYLLKRMKKTHSSINCAF
                     YSYIIMILLQYLVAGNASAFFFGPFLSVLIMCTPLILLHDTLKRLSRNENI"
BASE COUNT          168 a           78 c           69 g          147 t
ORIGIN      
        1 tgatattttc atttcgtgat aatctcttta acgtatcatg caataagatc agaggagtac
       61 acattatcaa tacggagaga aaaggaccaa aaaagaaggc cgatgcattc ccagccacta
      121 agtattgcaa taaaatcata atgatatatg aatagaatgc gcaatttatc gagctatggg
      181 tttttttcat tctctttaaa agataaaaag gtatatatat ccctaaaggg ataatgaata
      241 cacagagaaa accaaaaaaa actaacagtt ctgctccata cgtagtaact gataaaaaat
      301 ttgctcccat gtctccaaat acttgtttat gtaaccgttc tccataaaat aagtcttgat
      361 gctttattcc ctgtattcta tttattattg cttttaattc cttttctaac tctggtgtcg
      421 gaaagaaatc gttcttaata gattcagaaa caacatatgg aa
//
LOCUS       DAZFBR010000482          462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFBR010000482 DAZFBR010000000
VERSION     DAZFBR010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.488"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            139..>462
                     /gene="ler"
                     /locus_tag="ACHJZ2_005509"
     CDS             139..>462
                     /gene="ler"
                     /locus_tag="ACHJZ2_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO4571361.1"
                     /translation="MNMENNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDF"
BASE COUNT          156 a           76 c          102 g          128 t
ORIGIN      
        1 tgtttgatga aatagatgtg tcctaatttg atagataaac gttatctcac ataatttata
       61 tcatttgatt aattgttggt ccttcctgat aaggtcgcta atagcttaaa atattaaagc
      121 atgcggagat tatttattat gaatatggaa aataattcac atacaacaag tccatacatt
      181 cagcttatag agcaaattgc agttctacag caggaagcaa agcgactgcg agagcaggaa
      241 gttcaaagtg taattgagtc gattcagaag cagattactt attacaatat aaccttacaa
      301 gagctgggat atactaatgt gcctgatgat ggactcgctc gccggaactc atcgaaaggt
      361 gtttactacc gcaatgaaga agggcagacc tggtcgggcg taggccgaca gccacgctgg
      421 cttaaagaag cactgttgaa tggaatgaag aaagaagatt tt
//
LOCUS       DAZFBR010000483          461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFBR010000483 DAZFBR010000000
VERSION     DAZFBR010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.489"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>461
                     /locus_tag="ACHJZ2_005510"
     CDS             <1..>461
                     /locus_tag="ACHJZ2_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO4571362.1"
                     /translation="FENCYFDDCKFNNIQLYETNDDTVESGNKKPIKGMFKGCFISKC
                     KIENYRCETSKIYTVTQPDSIQEKLGSYLFMQSFVQDCIIQGGCCPGSSILLSHFYNC
                     NIIGLDAHGMDFLANSFNKSNYDEIRGPDTGTVFYNCNLKHIKINDGLRED"
BASE COUNT          153 a           59 c           90 g          159 t
ORIGIN      
        1 tttttgaaaa ttgctatttc gatgattgta aattcaacaa catacagctt tatgagacta
       61 atgatgatac tgtggaatca ggaaataaaa aaccaataaa aggtatgttt aaaggttgtt
      121 ttatttctaa atgcaaaatt gaaaattata gatgtgagac gtctaaaatc tacaccgtta
      181 cacaacctga tagtattcaa gaaaaattag gttcttatct ctttatgcag tctttcgttc
      241 aggattgtat aatccaggga ggatgttgtc ctggctcgag tatacttctg agtcattttt
      301 ataactgtaa tataataggg cttgatgctc atggaatgga ttttttggca aattcgttca
      361 acaagtctaa ttatgatgaa atcaggggtc cggatacggg gactgtgttt tataattgta
      421 atttgaagca tattaaaata aacgatggtc ttcgtgaaga t
//
LOCUS       DAZFBR010000484          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFBR010000484 DAZFBR010000000
VERSION     DAZFBR010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.490"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..288
                     /locus_tag="ACHJZ2_005511"
     CDS             <1..288
                     /locus_tag="ACHJZ2_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571363.1"
                     /translation="VIKNKNGNDYLSLGYYDQDGYHIQKGFRIDGDNLTQYCSKKARS
                     ASAWFEHSKAIIAESFATGSDHQVVNELKGERLRELNEAFNPSNLARGYEY"
BASE COUNT          181 a           69 c           86 g          121 t
ORIGIN      
        1 gtaattaaaa ataaaaatgg aaatgattac ttatctctcg gttactacga tcaggatggc
       61 taccacattc aaaaaggatt tcgtattgat ggtgataatc tcacccaata ttgtagtaaa
      121 aaagccagga gtgcttctgc gtggtttgaa cacagtaaag caatcattgc agaatcattt
      181 gcaacaggtt ccgatcatca agttgtaaac gaactcaaag gggaaagact gagagaactt
      241 aacgaagctt ttaatccttc aaatctagca agaggatatg aatattaaga ggatgataga
      301 aaatatacat ttgagaatac aaaagaaata gtttctactt tagtcggtaa caaagttaac
      361 gttggccatt ctcaaaaaat atataaacat tttaaggatc tcgaaggtaa aactgaagaa
      421 aggcttcaaa atcgccaggc tgaatatcaa aatgaaa
//
LOCUS       DAZFBR010000485          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFBR010000485 DAZFBR010000000
VERSION     DAZFBR010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.491"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..70
                     /locus_tag="ACHJZ2_005512"
     CDS             <1..70
                     /locus_tag="ACHJZ2_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571364.1"
                     /translation="LMLVALFLSMAWMALTSAGLVG"
     gene            82..387
                     /locus_tag="ACHJZ2_005513"
                     /pseudo
     CDS             82..387
                     /locus_tag="ACHJZ2_005513"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            329..>448
                     /locus_tag="ACHJZ2_005514"
     CDS             329..>448
                     /locus_tag="ACHJZ2_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFO4571365.1"
                     /translation="MPLVVSACASTPTVQAPCVKPPSPPAWIMQPVPDWQKPLN"
BASE COUNT          100 a          115 c          126 g          107 t
ORIGIN      
        1 actaatgctg gttgctcttt ttctttccat ggcctggatg gcgttgactt cggcagggct
       61 ggtggggtga gcataaaccg aatgctttcc gcgtttaccg ttattctgct ggtggtctgt
      121 ggtgcgctta gtctggggct gaatcattac cgcgataacg ccataaccta caaagagcag
      181 cgcgataaaa aagtcagtga gctggagctg gcaaatgcaa ccattactga tatgcagcag
      241 cgccagcgtg atgttgctgc acttgatgcc agatactcga gggaattagc tgatgcgaga
      301 gctgaaaatg aaactctgcg tgctgatgtt gccgctggtc gtaagcgcct gcgcatcaac
      361 gccaactgtc caggctcctt gcgtaaagcc cccatcacct ccggcgtgga taatgcaacc
      421 ggtccccgac tggcagaagc cgctgaac
//
LOCUS       DAZFBR010000486          438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFBR010000486 DAZFBR010000000
VERSION     DAZFBR010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.492"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            20..385
                     /locus_tag="ACHJZ2_005515"
     CDS             20..385
                     /locus_tag="ACHJZ2_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4571366.1"
                     /translation="MAVLRTLPGRIKTLNTRRINVLRGEQRRVSGSARVSLKRRIWRR
                     DAGHCCLCRRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSASEAAGGMPD
                     PTLPELPDGTLRADGITGL"
BASE COUNT           94 a          113 c          137 g           94 t
ORIGIN      
        1 tatatggcga ggattttaca tggctgtgct tcgtacgtta ccgggcagaa tcaaaactct
       61 gaacacccgg cggataaatg tcctgagggg ggaacagcgt cgtgtcagtg gcagtgcccg
      121 ggtttccctc aaacgtcgta tctggcggag ggatgccgga cactgttgtc tctgtagacg
      181 cgtggttgac ctttgtgaca gtgaactcga tcaccgcatt gcacttcagt tcggtggtgg
      241 taatgaggag acgaacctct ggaccctctg tactgaatgc catcgccaga agtcagcgag
      301 tgaagcggcg ggtggtatgc ctgatccgac gttgcctgag cttcctgatg gcacgctcag
      361 ggccgacgga atcactggcc tgtgaccaga cccggggggg atcatccggc gaaaaaaaac
      421 gatcgccctg gacaccgc
//
LOCUS       DAZFBR010000487          434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFBR010000487 DAZFBR010000000
VERSION     DAZFBR010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.493"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>434)
                     /gene="espL2"
                     /locus_tag="ACHJZ2_005516"
     CDS             complement(<1..>434)
                     /gene="espL2"
                     /locus_tag="ACHJZ2_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO4571367.1"
                     /translation="EYLLSAFKYEAHSSNKYTPGLFSAFQNGHADAIKAYCGVLGNSN
                     LKRGEIIRMLEARNYDGAPGLLLAYQNGDINTIQSFFDSLIMLDISKDFIEELLTAKH
                     YDFTGLSLAISHRHDHVVKLYGKLFKKLDTSPYKMSIILALA"
BASE COUNT          143 a           82 c           61 g          148 t
ORIGIN      
        1 tgctaatgct aggataattg acattttata tggactggta tcaagttttt taaataactt
       61 tccgtataat ttgacaacat gatcatgtct atgactgatc gctaatgata accccgtaaa
      121 atcataatgt tttgcagtca ataactcttc gataaaatcc tttgatatat ctaacattat
      181 tagtgagtca aaaaaagact gaatcgtatt aatatctcca ttttgatacg caagaagtaa
      241 tcctggtgca ccatcataat ttctcgcctc aagcatcctg attatctcac ctcttttcaa
      301 attactattg ccaagtacgc cacaatatgc ctttattgcg tctgcatgac cattttgaaa
      361 tgcagaaaat aatccaggag tatatttatt agagctatgt gcctcatatt taaatgctga
      421 aagcaaatac tcta
//
LOCUS       DAZFBR010000488          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFBR010000488 DAZFBR010000000
VERSION     DAZFBR010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.494"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..140
                     /gene="escE"
                     /locus_tag="ACHJZ2_005517"
     CDS             <1..140
                     /gene="escE"
                     /locus_tag="ACHJZ2_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFO4571368.1"
                     /translation="KKNEIHEKMKHPLDKVTYNEAKELLIACDAAIRTIEIMRIRINN
                     K"
     gene            153..>431
                     /gene="cesAB"
                     /locus_tag="ACHJZ2_005518"
     CDS             153..>431
                     /gene="cesAB"
                     /locus_tag="ACHJZ2_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesAB"
                     /protein_id="HFO4571369.1"
                     /translation="MSQTRNKELLDKKIRSEIEAIKKIIAEFDVVKESVNELSEKAKT
                     DPQAAEKLNKLIEGYTYGEERKLYDSALSKIEKLIETLSPARSKSQSTM"
BASE COUNT          196 a           58 c           87 g           90 t
ORIGIN      
        1 acaaaaagaa cgaaatccat gaaaaaatga aacacccttt ggataaagtt acctacaatg
       61 aagcaaagga actgctcatt gcgtgtgatg cggcaattag gactatagag ataatgcgaa
      121 tcaggattaa taataaatag aggatgagta ttgtgagcca aacaagaaat aaagaactat
      181 tagataaaaa aataagatca gaaattgagg cgataaaaaa aataatcgcc gaatttgatg
      241 tcgtaaaaga aagcgttaat gagttaagtg aaaaagcaaa aacagatcct caggcggcag
      301 aaaaattaaa caaattaatt gaggggtaca catatggtga agagcgaaaa ttatatgatt
      361 ctgccctaag taagattgaa aaactaatag agacactgag tccagcaaga tctaaaagcc
      421 aatcaacaat g
//
LOCUS       DAZFBR010000489          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFBR010000489 DAZFBR010000000
VERSION     DAZFBR010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.495"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            97..>430
                     /locus_tag="ACHJZ2_005519"
     CDS             97..>430
                     /locus_tag="ACHJZ2_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571370.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNTKTTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDSLAQMQYWTAVRNIIDDTNEVTIDLSYNLAIT
                     NIDTSDEHL"
BASE COUNT          162 a           87 c           57 g          124 t
ORIGIN      
        1 tataaactga aatttcttaa tcttgattat aacaagctag catcgctgga tgtctcccgg
       61 caagagtctc tgattgagtt aagtgcccat cacaatatga tcactgacct tatattacac
      121 aatcatccca ggatgaaaac aatcacttta aacgacaacc atattgcaca tttaaacacc
      181 aaaactacta caaaacttga atatttaaac ttaagcaata acaatttatt gccaaccaat
      241 gacattgatc aactaatatc atcaaaacat ctttggcatg tattagttaa cggcatcaac
      301 aatgattcac ttgcacaaat gcagtactgg actgcagtaa gaaatataat tgatgacact
      361 aatgaagtga ccattgattt atcttataat ctggcaatca caaatatcga taccagtgat
      421 gaacatcttg
//
LOCUS       DAZFBR010000490          427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFBR010000490 DAZFBR010000000
VERSION     DAZFBR010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.498"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>427
                     /gene="espX5"
                     /locus_tag="ACHJZ2_005520"
     CDS             <1..>427
                     /gene="espX5"
                     /locus_tag="ACHJZ2_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFO4571371.1"
                     /translation="DRITRRVYLNKDKEVSIKVVYFINNVTVHNNTIEIPQTVNGGYD
                     FSHLSLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFNFAIMEKILFDNCILDD
                     SYFAQIKMTDGTLNSCSAMHVQFYNATMNRANIKNTFLDY"
BASE COUNT          153 a           70 c           69 g          135 t
ORIGIN      
        1 cgatagaatt acgagaagag tttaccttaa taaagataag gaagtgtcaa ttaaggtggt
       61 atattttata aataatgtca ccgtccataa taatactatc gaaatcccac agacagtgaa
      121 tggtggttac gatttttcac accttagcct gaaaggtatc gtgattaaag atgaagattt
      181 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
      241 gtataaaacg aattttaatt tcgccataat ggaaaaaata ctttttgata attgtattct
      301 cgatgactca tatttcgctc agataaaaat gactgacgga actctaaatt catgctccgc
      361 tatgcatgtt caattctaca atgcaacaat gaatagagcc aatattaaaa ataccttcct
      421 tgattat
//
LOCUS       DAZFBR010000491          426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFBR010000491 DAZFBR010000000
VERSION     DAZFBR010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.499"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
BASE COUNT          147 a           72 c           68 g          139 t
ORIGIN      
        1 caacgaaatg atttttacaa cataccatta tcgctaaaca aaatcgcacc catcaaatca
       61 acttacgtta caatcactac aaagcgattg catttaaatt caaaacagtt ttctgttggg
      121 cgcatttttt gttgtactgg actaaagaga aaaaatgggg taaggaacaa ataccttccg
      181 tgctcaaaaa ttaggttttt atgcacttat taatcagtca aggtgaacag taaagtgttc
      241 acttcttatc aatcgttaat ttcatatctt tttgaatatt aatgaaaaaa aacacaatga
      301 gtagtatgaa cagttttccc ccaaaaaaag ttttttctgc cttgagaatg cgcggaaagc
      361 gaacgccttc accaatgtgt ataggggtgt gtgtagttta aatcttctat tttaatttat
      421 gtatta
//
LOCUS       DAZFBR010000492          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFBR010000492 DAZFBR010000000
VERSION     DAZFBR010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.500"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            2..154
                     /locus_tag="ACHJZ2_005521"
     CDS             2..154
                     /locus_tag="ACHJZ2_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4571372.1"
                     /translation="MVNGMDSIKLLDKVIIYQQQNEQLYPAQEHLLLQLCMRVTKKLT
                     DILMPP"
     gene            184..>421
                     /locus_tag="ACHJZ2_005522"
     CDS             184..>421
                     /locus_tag="ACHJZ2_005522"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO4571373.1"
                     /translation="MLLALLSSTDNFCLSSTELSERLDVSRTYITRACDSLEKFGFIK
                     RMESKEDRRSKNIYLTSDGNLYLQRTTRIYGRYLK"
BASE COUNT          145 a           66 c           76 g          134 t
ORIGIN      
        1 aatagttaat ggaatggata gtattaaatt acttgataaa gttattatct atcaacaaca
       61 gaatgagcaa ttatatccag cacaggaaca tcttctttta caactttgta tgcgagttac
      121 caaaaaactt actgatatat taatgcctcc ctgaaggggg atggtataaa cgatacaacg
      181 cttatgcttc tagccttgtt atccagtact gataatttct gcttatcttc gactgaactc
      241 agtgaaagat tagatgtttc cagaacatat atcaccagag cttgtgattc tctggaaaaa
      301 tttggtttta ttaaacgtat ggaaagtaag gaagatcgac gtagtaagaa tatttatctg
      361 acttcagacg gtaatttgta tttacagcgg accacaagga tatatggacg ctatttaaaa
      421 a
//
LOCUS       DAZFBR010000493          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFBR010000493 DAZFBR010000000
VERSION     DAZFBR010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.501"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>420
                     /locus_tag="ACHJZ2_005523"
     CDS             <1..>420
                     /locus_tag="ACHJZ2_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4571374.1"
                     /translation="FGAMTIDTIDDKAIYQLNVYLGSERNYKLNLIKAFIIKWKNLNY
                     PGVEATAIRMLEKIKIIPNQTGDAVKRRDPNKGPLTEAEFNNIINAVGKFYHEKKIQC
                     FLYCYILLLAITGRRPLQLISLKAKDLIKNERGCFLN"
BASE COUNT          162 a           62 c           77 g          119 t
ORIGIN      
        1 ggtttggtgc gatgactatc gatactattg atgacaaagc aatatatcaa ttgaatgttt
       61 atttaggttc agaaagaaac tacaaactaa acttaattaa ggctttcatc attaaatgga
      121 aaaatctcaa ttaccctggg gtagaagcga ctgccattag aatgctggag aaaataaaaa
      181 tcattccaaa ccaaacagga gatgcagtta aaagacgaga tccaaataaa ggacctttaa
      241 ctgaagcgga attcaataac atcattaacg ccgttggaaa attttatcat gagaagaaaa
      301 ttcaatgctt tttgtattgt tatatccttt tgctggcaat aacaggaaga aggccattac
      361 aattaatatc tctaaaagct aaagatctca ttaaaaatga gagagggtgc tttttgaatg
//
LOCUS       DAZFBR010000494          419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFBR010000494 DAZFBR010000000
VERSION     DAZFBR010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.502"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            178..>419
                     /locus_tag="ACHJZ2_005524"
     CDS             178..>419
                     /locus_tag="ACHJZ2_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571375.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQA"
BASE COUNT          121 a           77 c          114 g          107 t
ORIGIN      
        1 ttttcgctat ttatgaaaat tttccgggat ccatgtccgg tttctcttca agttaactat
       61 atgaaaaata taaaaacagg tcttctgtga gccggacatg aacaaaaaac agacatgtaa
      121 accggacatg accggttttg ttgtgattgt gaggtgagag tttttgcgag gtgaggagtg
      181 gctacgcaga ctgaagttgc caggcattta agtctgaccg atcgccagct tcgcagattg
      241 cagaaattgc cgggtgcccc gatatcgaat aagcgagggc aactggatct ggatgcctgg
      301 cgcgattttt acatatcgta tctgaggaga agtaaaaacg atgtgcctga tggcgatagc
      361 gaagacgact atgaggagaa attgcttatt gccagatggg aactgacagc agaacaggc
//
LOCUS       DAZFBR010000495          416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFBR010000495 DAZFBR010000000
VERSION     DAZFBR010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.503"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>416
                     /locus_tag="ACHJZ2_005525"
     CDS             <1..>416
                     /locus_tag="ACHJZ2_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO4571376.1"
                     /translation="SDKIALSINDKKYNVNSKDIENILDGKGDLFKKRTLWEFVRDLF
                     PGSHIKEVKGLIYEFVTKVDNKAEVFDKIKSLAKKEQQWRFSTKTDFTTNENNEVIVS
                     RSFNLYTGATSNDNEKKQVSSERLTLDNYLDDLHFD"
BASE COUNT          154 a           55 c           77 g          130 t
ORIGIN      
        1 tctgataaaa ttgctttaag tattaatgat aagaaatata atgtaaactc taaggatatt
       61 gaaaatattc ttgatggcaa aggggattta tttaaaaaac gtacactgtg ggagtttgtt
      121 cgcgatcttt ttcctggctc tcatattaaa gaagtcaaag gcttaattta cgaatttgtc
      181 acgaaagttg ataataaagc cgaggtgttc gataagatta aatctctggc aaaaaaagaa
      241 caacaatggc gatttagtac taagacagac tttaccacaa atgaaaataa tgaagttatt
      301 gtgagtagat cctttaatct ctacacgggg gctacttcga atgataacga aaaaaaacag
      361 gtttcttcag agagattaac gcttgataat tatctcgatg atttacattt tgataa
//
LOCUS       DAZFBR010000496          415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFBR010000496 DAZFBR010000000
VERSION     DAZFBR010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.504"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>415
                     /locus_tag="ACHJZ2_005526"
     CDS             <1..>415
                     /locus_tag="ACHJZ2_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4571377.1"
                     /translation="FFRLSHRPSWRYLGIGEEEARAFSREVEAAWKEFAEDDCCCIDV
                     ERKRTFTMMIREGVAMHAFNGELFVQATWDTRPSRLFRTQFRMVSPKRISNPNNTSDS
                     RNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTW"
BASE COUNT           90 a          109 c          129 g           87 t
ORIGIN      
        1 cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag
       61 cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgaa gatgactgtt
      121 gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca
      181 tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggatacccgt ccctcgcgac
      241 tgttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaatacca
      301 gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat
      361 attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca tggat
//
LOCUS       DAZFBR010000497          401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFBR010000497 DAZFBR010000000
VERSION     DAZFBR010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.505"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..356)
                     /locus_tag="ACHJZ2_005527"
     CDS             complement(<1..356)
                     /locus_tag="ACHJZ2_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFO4571378.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRR"
BASE COUNT          136 a           83 c           56 g          126 t
ORIGIN      
        1 aatcgccgct gtaacaattc aattctgtta tcattactat gcataaattc agaaataacc
       61 tgccccatat atgtactgaa tacatcaaga aatgaattgc ctccattcaa ttgattttca
      121 acagaatcag ccaatctaat gatatcggct agttctgtat ggcttaaacc cgaatatagt
      181 tccccttgaa caggatctac aataaaagcg tccatagcac tctgatagcg aacagaaaac
      241 gatctttctc ctattgtgag tctttccatt tgatgatctc tggtcgcacg ttgtaaaact
      301 tcaacatctt gtgttgacaa gtaatttcca gaagacaaat taaatgaaaa tggcataatt
      361 ttatccctaa agcatatatc ttacttttcg ataattaaca a
//
LOCUS       DAZFBR010000498          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFBR010000498 DAZFBR010000000
VERSION     DAZFBR010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.506"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
BASE COUNT          133 a           79 c           73 g          114 t
ORIGIN      
        1 tcttatatac atttaagtag tgatgtatca cgtaaaaata tagtcatcat agatgttatg
       61 cagtacgcac aaaaaaacag actggatttg cttgctgatc tataatcttt atctaaatta
      121 ctattggcgc tttggataat aacataagca tatattacct cctagcaata atagatataa
      181 cctcatatca aggtgtcact ggtaaataat cggataaaag acagtaccgg tcggcacata
      241 gagcttcttc tatttcacca gaagtgcaca gcagggaact accaatcaca cggttagtgt
      301 catgggtgga ggatgcgtcc cgttctttcc ctataacggg gatacgcagt ttttatcagt
      361 acatgcagaa aaacccctaa ttcacatcac tgcagaaag
//
LOCUS       DAZFBR010000499          392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFBR010000499 DAZFBR010000000
VERSION     DAZFBR010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.507"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>392
                     /locus_tag="ACHJZ2_005528"
     CDS             <1..>392
                     /locus_tag="ACHJZ2_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4571379.1"
                     /translation="NNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDG
                     ENAIVDNSKPAWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVL
                     DCLKNNSCFNYYYISAPSLLWQNERIIK"
BASE COUNT          111 a           80 c           71 g          130 t
ORIGIN      
        1 ccaacaatta catttctgat attctgcctg ttattgatgc actacccaat ccaccagtgt
       61 tagttacact tggttatgaa tcttggaata accttagcat tcatcgccgt gcttacgatt
      121 atactcctga tggtgaaaat gctattgttg ataactctaa accagcatgg atctacttta
      181 ctggtggagg aagccagtct ttccgagaat tattactaac tcagatcatg ccatgggtta
      241 gtactattgc tccgaacagt tccagaatcg gtatatgggg gcactcgcta ggtgctattt
      301 ttgtgcttga ttgcttaaaa aataattcat gctttaacta ttattatata tctgcaccat
      361 cacttctatg gcagaatgaa agaatcatca ag
//
LOCUS       DAZFBR010000500          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFBR010000500 DAZFBR010000000
VERSION     DAZFBR010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.508"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..167
                     /gene="ninE"
                     /locus_tag="ACHJZ2_005529"
     CDS             <1..167
                     /gene="ninE"
                     /locus_tag="ACHJZ2_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4571380.1"
                     /translation="TPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDKKW
                     LRLAARRNHG"
     gene            160..>389
                     /locus_tag="ACHJZ2_005530"
     CDS             160..>389
                     /locus_tag="ACHJZ2_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO4571381.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECA"
BASE COUNT          111 a          102 c           94 g           82 t
ORIGIN      
        1 ctacaccgct tattcgtgtc atgaacggac acatctacaa agtaccaaat cgtcgtaagc
       61 gtaaacctga gctgaagcca tccgaaatac caacactgct cggatatacc gccagcctgg
      121 ttgataaaaa atggttgcga ctggcagcaa ggaggaatca tggctgattt gagaaaagca
      181 gcgcgtagtc gggaatgcca agtaagaatc cctggcgtat gtaatggcaa ccctgaaacg
      241 tctgtactgg cacatatccg gctgactgga ttgtgcggca ccggtaccaa accgccagac
      301 ttgattgcca ccattgcatg ttctgcctgc cacgacgaaa tcgaccgccg cacacatttt
      361 gtcgatgctg catatgcaaa agaatgcgc
//
LOCUS       DAZFBR010000501          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFBR010000501 DAZFBR010000000
VERSION     DAZFBR010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.509"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>389
                     /locus_tag="ACHJZ2_005531"
     CDS             <1..>389
                     /locus_tag="ACHJZ2_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO4571382.1"
                     /translation="IDKHLNLNHCCLYAACLGELTGGNPLYDCAIDTHTTILSGNLAA
                     NTQVKIPVYMYHRGAIAEKDILPFMEKVTNIYSRIEDIYKYSNNMKNNYKKALADLQS
                     LLVNLHNIMPGDDVKDFQLNDETIKYI"
BASE COUNT          137 a           56 c           65 g          131 t
ORIGIN      
        1 tatagataaa catttaaatc ttaatcattg ctgtttatat gctgcttgtt tgggcgagtt
       61 aaccggtggg aatcccttgt atgattgtgc tattgatact cataccacta tcttaagtgg
      121 taatcttgct gcaaacactc aggttaaaat tcctgtttat atgtaccata gaggagctat
      181 agctgaaaaa gacattttac ctttcatgga aaaggtaacg aatatttatt cacggataga
      241 agatatttat aaatatagca ataatatgaa gaataattac aaaaaggcac tagccgattt
      301 acaatcctta ttagttaatc tgcataacat tatgcctggt gatgatgtaa aagattttca
      361 gctcaatgat gaaactataa aatatatta
//
LOCUS       DAZFBR010000502          377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFBR010000502 DAZFBR010000000
VERSION     DAZFBR010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.510"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>377)
                     /locus_tag="ACHJZ2_005532"
     CDS             complement(<1..>377)
                     /locus_tag="ACHJZ2_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4571383.1"
                     /translation="HARRCAPAESCGFVIGTPEGERYQPCVNISAEPEAYFRIAPEDW
                     LQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHKFRCVPHLTGR
                     RFEHGVTDCYTLFRDAYHLAGIT"
BASE COUNT           73 a          136 c          105 g           63 t
ORIGIN      
        1 gcgttatccc cgccagatgg tatgcatccc ggaacagggt gtaacagtcc gtcaccccgt
       61 gctcaaaacg acgcccggtc aggtgcggca cacagcggaa tttgtgaatt tccccccggc
      121 agaccagcca ccagggcagg gcactcttta tctgcagccg ccggtcggcc tcgctcagcc
      181 agggcagacc accagggtgg ctgtgaacca gcgccacaat ctccccctgc atctctgcct
      241 gcagccagtc ttccggcgca atacgaaaat acgcctccgg ctctgcggag atattcacgc
      301 agggctggta ccgttcgccc tccggggtgc ctatcacgaa gccgcacgac tctgcaggcg
      361 cacaccgccg ggcatgc
//
LOCUS       DAZFBR010000503          375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFBR010000503 DAZFBR010000000
VERSION     DAZFBR010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.511"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>375)
                     /locus_tag="ACHJZ2_005533"
     CDS             complement(<1..>375)
                     /locus_tag="ACHJZ2_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571384.1"
                     /translation="DVFIKIFESWVTKLPSISGPVNLHIPTSFKDKSLEVESYFVDKS
                     IWNVHIAYHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNHCFSPDKVNEICEQARH
                     YLVEKMCETHSLAMNNSVLTNP"
BASE COUNT          133 a           64 c           58 g          120 t
ORIGIN      
        1 tcagggtttg tcaaaacaga attattcata gccaaagaat gtgtttcaca cattttttca
       61 actaaataat gcctggcttg ttcgcagatt tcgttaacct tgtcagggga gaaacaatga
      121 ttattaatta aatattgctc acaattatcc acaaattcct gcggactaaa ttcagcaata
      181 aattgatcgg taaaaaacac aaaacgctta tcatcatgat atgcaatatg gacattccat
      241 attgacttgt cgacaaaata tgactctact tcaagagact tatctttgaa actggtaggt
      301 atatggagat ttacaggacc tgatattgat ggtaattttg ttacccaact ttcaaaaatt
      361 ttgataaaaa catca
//
LOCUS       DAZFBR010000504          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFBR010000504 DAZFBR010000000
VERSION     DAZFBR010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.512"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            1..283
                     /locus_tag="ACHJZ2_005534"
                     /pseudo
     CDS             1..283
                     /locus_tag="ACHJZ2_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            280..>371
                     /locus_tag="ACHJZ2_005535"
     CDS             280..>371
                     /locus_tag="ACHJZ2_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571385.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGD"
BASE COUNT          108 a           84 c           94 g           85 t
ORIGIN      
        1 tattgagcgc cgtgcttatg aaattgcact ggcatcgctg gaagcagaat cgatagcgtg
       61 agaatgcggt gaaaacataa tcctgtttaa ccctgacaca gttgaagcat acgcaaaacg
      121 tgcggaaata tcacctaaac cactattctc cgcgccgcca gcgctggtag tgcctgataa
      181 gttgccgcgt gaatacagaa acggttggcc tcttgcgtat agtgattatg ctgaaggctg
      241 gaacgactgc cgcgaagcca tgcttcaggg agataaatca tgattaatcg tatcaagctg
      301 gagcacatcc tcgaatatgc caggcagcag aggcatattg gtcagcattg taaaattcca
      361 ccaggagata t
//
LOCUS       DAZFBR010000505          369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFBR010000505 DAZFBR010000000
VERSION     DAZFBR010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.513"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..309)
                     /locus_tag="ACHJZ2_005536"
     CDS             complement(<1..309)
                     /locus_tag="ACHJZ2_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO4571386.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIWINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     I"
BASE COUNT          125 a           54 c           70 g          120 t
ORIGIN      
        1 gatatctatc tcatcatggg taagtggttc ttgtgtggaa tacacatttt gtgtatgaag
       61 atttgaaacc aggaaatgcg ttaaatcaga taatgtaata ttcttattga tccagatgaa
      121 attgttactc aaaaggatgt ttacaggttc tgtatttggt ttgagatttc gatgttgatg
      181 ctcatcaata acaacgaacg aatgtccaag atcaaccaac gcaactaaat cgctgtaata
      241 ttccttttgg gatgctaata atgcattagt gcctacagtg aagtagacat cactggaaaa
      301 gacttgcata tgccaacctg tactctaaaa tcatttaggg taaatattat gtgtaaaatt
      361 taaaataag
//
LOCUS       DAZFBR010000506          365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFBR010000506 DAZFBR010000000
VERSION     DAZFBR010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.514"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>365)
                     /locus_tag="ACHJZ2_005537"
     CDS             complement(<1..>365)
                     /locus_tag="ACHJZ2_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4571387.1"
                     /translation="GLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQ
                     ELTDRWVVEQMSSLTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGP
                     AVADEFDNPTTDIRKDRCS"
BASE COUNT           69 a          138 c           91 g           67 t
ORIGIN      
        1 ttactgcatc tgtccttccg gatatccgtg gtggggttgt cgaactcatc cgccactgcc
       61 ggaccgttat acccgcattc atcgccccgg taatcccaca tacaggtgtt cgccagcatg
      121 atgcgaccgg gaaacagcgc cccgtccgtc tccgtcggtg tcgccagcac aaacgaggcc
      181 gtcatggccg tcagcgatga catctgctcc accacccacc ggtccgtcag ctcctgctca
      241 gggtctgcct caggattgcc tgccacaaaa ttcaccgcat ccagaaaacg ggcatacacc
      301 cggcggcgga ccaccgtggc ccccaccagg ctctgcaggt cctccgccat cccggtgacc
      361 agacc
//
LOCUS       DAZFBR010000507          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFBR010000507 DAZFBR010000000
VERSION     DAZFBR010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.515"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            273..353
                     /locus_tag="ACHJZ2_005538"
                     /pseudo
     CDS             273..353
                     /locus_tag="ACHJZ2_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           83 a          102 c           66 g          102 t
ORIGIN      
        1 aaatgcgaat tatttagtac aaaaagtgaa cttttgatga tctttcccta atctcacata
       61 ggatcagatc tcgtctgatc gttccccctg cgctcactac catggctctt taccacagca
      121 gccggggttg tttgggaccg acttccgaca gcagatcccg gtgggcctgc caccatctct
      181 tcacaagctt atgcttatta catgcaaaac gtcccgtttt gcatctttgt gcctacggca
      241 caccgatgat ctttccctgt gatctttccc tgaatctgcc cctgagaacc tgaatcaaac
      301 gtctgactcg cagaaccatc tgtttctcgc gctctgtaga acttcatgaa aaa
//
LOCUS       DAZFBR010000508          341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFBR010000508 DAZFBR010000000
VERSION     DAZFBR010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.516"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>341
                     /locus_tag="ACHJZ2_005539"
     CDS             <1..>341
                     /locus_tag="ACHJZ2_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4571388.1"
                     /translation="LVFHTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSL
                     SRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAE
                     VLQEDTGVTLPA"
BASE COUNT           79 a           90 c          126 g           46 t
ORIGIN      
        1 ctggtgttcc acacgcggat acagcacggc gaaccacagg tggagagccg gtacctctat
       61 gacccgctgg gccgccggac gggaaaacgg gtgtggcggc gggagcgtga cctgacgggg
      121 tggatgtcgc tgtcgcgtaa accggaggag acctggtacg ggtgggacgg tgacaggctg
      181 accactgtac agacccaaca gacaagaatc cagacggtat accagccggg aagcttcacg
      241 ccgctcctga gaatcgaaac agagaatggt gaacaggcga aggcgcggca ccgtagcctg
      301 gcggaggtgt tgcaggagga cacgggtgtg acgctaccgg c
//
LOCUS       DAZFBR010000509          334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFBR010000509 DAZFBR010000000
VERSION     DAZFBR010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.517"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            20..>334
                     /locus_tag="ACHJZ2_005540"
     CDS             20..>334
                     /locus_tag="ACHJZ2_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571389.1"
                     /translation="MKVFHNIFKYISSNHQDKHSDKVNNHQHHGKVDKKHRSEIVEID
                     KLDKHSQIDNDFGLHIIYFLQHGCWNVTEHSPQIEKIWFYNSEPSIDIKEYNSFADNT
                     TDT"
BASE COUNT          128 a           56 c           54 g           96 t
ORIGIN      
        1 aaaatatcac gggttattta tgaaagtatt tcacaatata tttaagtaca tctcttcaaa
       61 ccatcaagat aagcattcag ataaagttaa taatcatcaa caccatggca aggttgataa
      121 aaaacatcgt tcagagatag ttgaaattga taagctggat aaacattcgc aaatagataa
      181 cgattttggc ctgcatatca tctatttcct gcaacatggc tgctggaacg taactgaaca
      241 tagtcctcag atagagaaaa tatggtttta caatagtgag ccatctatag atattaaaga
      301 atataatagt ttcgccgaca atactactga cacg
//
LOCUS       DAZFBR010000510          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFBR010000510 DAZFBR010000000
VERSION     DAZFBR010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.518"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>333
                     /locus_tag="ACHJZ2_005541"
     CDS             <1..>333
                     /locus_tag="ACHJZ2_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4571390.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAK"
BASE COUNT           74 a           85 c          103 g           71 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaa
//
LOCUS       DAZFBR010000511          332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFBR010000511 DAZFBR010000000
VERSION     DAZFBR010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.519"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
BASE COUNT          102 a           58 c           71 g          101 t
ORIGIN      
        1 ttagtttttg aagaccaccg caagtgttcg tctggcttca catggcatct tcttcttaga
       61 aaaagatcga catattttgt gacacgaatt gcaaatctgg ttttgttgta tggattgcgt
      121 gattttcgat ctggtataac aggtataaag gtgcaccacg atagacaatg agacagggca
      181 tcttgcaatc tatggcaaac atcacttcag ttctttctca tcgggtgatg aaaacgcact
      241 tcagcctgaa aggaatatga aaatgagatc aacagacact ctattttatg actctgggta
      301 aaatgaattg agtaagtgat atagcttacg aa
//
LOCUS       DAZFBR010000512          325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFBR010000512 DAZFBR010000000
VERSION     DAZFBR010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.520"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>325
                     /locus_tag="ACHJZ2_005542"
     CDS             <1..>325
                     /locus_tag="ACHJZ2_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO4571391.1"
                     /translation="ADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKAL
                     FMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWG
                     TLKNE"
BASE COUNT           87 a           77 c           93 g           68 t
ORIGIN      
        1 tggcggacct gacgtatgtt gccacacagg agggatggtt gtacctcgct ggcatcaaag
       61 atgtttatac gtgcgaaatt gtcggctacg ccatgggaga gcgcatgaca aaagagctga
      121 caggtaaagc cctgtttatg gcgctcagga gccagcgccc acctgccggg ctaatccacc
      181 actctgatcg aggttcacag tactgcgcat acgattaccg ggtcatacag gagcagtttg
      241 gtctgaaaac atcaatgtcg cgtaaaggta actgttacga caacgctccg atggaaagct
      301 tctggggaac gctgaaaaat gagag
//
LOCUS       DAZFBR010000513          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFBR010000513 DAZFBR010000000
VERSION     DAZFBR010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.521"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..268
                     /locus_tag="ACHJZ2_005543"
     CDS             <1..268
                     /locus_tag="ACHJZ2_005543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571392.1"
                     /translation="LFILATFTYSVQSDEFDDQTTYTQYRYFSTAIPEDIRRFMFYVD
                     YCFVDLAFDIETDSMTAKNDIINECTPLKERNKTLKEKYKKMKR"
BASE COUNT          130 a           49 c           44 g          100 t
ORIGIN      
        1 actatttata ctggccacat ttacatattc agtacaatca gacgaattcg atgatcaaac
       61 cacttacacg caataccgtt atttttccac tgctataccg gaagatatac gtagatttat
      121 gttctacgta gattattgct ttgtagattt agcattcgat atcgaaactg actcaatgac
      181 agctaagaat gatattatta atgaatgtac tccattaaaa gaaagaaaca agacattaaa
      241 agaaaaatat aaaaaaatga agagataata gcaattcttt ccattgcaga tgatactatt
      301 ttaatttatg aagaattaaa aaa
//
LOCUS       DAZFBR010000514          320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFBR010000514 DAZFBR010000000
VERSION     DAZFBR010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.522"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(35..>320)
                     /locus_tag="ACHJZ2_005544"
     CDS             complement(35..>320)
                     /locus_tag="ACHJZ2_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4571393.1"
                     /translation="RPKRCRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGT
                     KAHDIFTLPLCREHHNELHADPLEFEKKYGSQIELIFRFLDHAFATGVLG"
BASE COUNT           82 a           93 c           71 g           74 t
ORIGIN      
        1 gtaaaaccaa atctatgagc atcagtcacc tcttttaccc gagcacgcca gtcgcaaagg
       61 cgtgatcaag aaaacgaaaa attaactcaa tctgagagcc gtactttttc tcaaactcca
      121 gcgggtctgc atgaagttcg ttgtggtgct cccggcacaa cggtagcgtg aaaatatcgt
      181 gggcctttgt ccccattccc ccctgaccat gaccaatcag gtgatgcgga tcgtcagcag
      241 gcttaccaca acacgcacac ggctgtgtct ttacccagcg cgtgtatttc tcattaaccc
      301 agcggcaacg tttaggccgc
//
LOCUS       DAZFBR010000515          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFBR010000515 DAZFBR010000000
VERSION     DAZFBR010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.523"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>306)
                     /locus_tag="ACHJZ2_005545"
     CDS             complement(<1..>306)
                     /locus_tag="ACHJZ2_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4571394.1"
                     /translation="GSWMAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQNMAAML
                     TGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTAIQAA"
BASE COUNT           66 a          114 c           71 g           55 t
ORIGIN      
        1 ccgcagcctg aatggccgtc cccgaggaag cagatgcacc accaccgaaa gcaccgccaa
       61 tggcgctgcc gatacgcccg acaatgccca ccatggcctg tttaagcagg atttctgtca
      121 tcatggacag caccgaacgg gtgaatcccc gccagtctgc ctctgcaccg gtcagcatcg
      181 ccgccatatt ctgtgcaata ccgtcaaagg tctgcgtggc agcactttta acctgcgaaa
      241 aactgtccgt cgcactttcc gcccactcgc cccagccgga cttcagcccg gccatccagc
      301 ttccac
//
LOCUS       DAZFBR010000516          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFBR010000516 DAZFBR010000000
VERSION     DAZFBR010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.524"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..286
                     /locus_tag="ACHJZ2_005546"
     CDS             <1..286
                     /locus_tag="ACHJZ2_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571395.1"
                     /translation="QWEQKFARHVQHVRAQVKPVSKGVSHAAPGGTASRAVQEIRAAR
                     EQWERENGFISNGNGLEAVGTYGGGVFEPLDPEERGCAVEALDCSDWRDD"
BASE COUNT           82 a           72 c          102 g           50 t
ORIGIN      
        1 tcagtgggag cagaaatttg cccgtcacgt gcagcacgtc agggcacagg taaaaccagt
       61 cagcaagggg gtaagccatg cagcaccagg tggcaccgca tcacgggcag ttcaggaaat
      121 tcgggcagca cgtgaacagt gggaacgtga aaacggattt atcagcaacg gaaacggcct
      181 ggaagctgtg ggaacttatg ggggaggtgt attcgaaccg ctggacccag aagaacgggg
      241 ctgcgccgtc gaagctctgg attgctcaga ttggcgcgat gactgaacag caaatccggc
      301 aggtct
//
LOCUS       DAZFBR010000517          304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFBR010000517 DAZFBR010000000
VERSION     DAZFBR010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.525"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..167
                     /locus_tag="ACHJZ2_005547"
     CDS             <1..167
                     /locus_tag="ACHJZ2_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4571396.1"
                     /translation="RKNIRQKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKKIIGS
                     SSGLSCFQPH"
BASE COUNT           98 a           49 c           58 g           99 t
ORIGIN      
        1 cccgcaaaaa cattagacaa aaatatggcc atcatgggtt aggggtaaat cagaatcaac
       61 aatttgataa tggtgttatg gggctaaatt ccaggatggc agtgtttgat gaaaataaaa
      121 aaataatagg ttcttcctca ggactaagtt gttttcaacc acattaaaac tctatgagtg
      181 ctgtggtata tctttttaat cctgccagta tttcatagtt tttcttaatc gattgtagag
      241 gcatgtactg atgacgcctc tacaatcttt agggactttc tctactatga gataagaaat
      301 ttca
//
LOCUS       DAZFBR010000518          299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFBR010000518 DAZFBR010000000
VERSION     DAZFBR010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.526"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(1..80)
                     /locus_tag="ACHJZ2_005548"
                     /pseudo
     CDS             complement(1..80)
                     /locus_tag="ACHJZ2_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          101 a           47 c           62 g           89 t
ORIGIN      
        1 cgggcatcct cttcaaggat ggtgatcctc aaccgcaccc atttaggatg cgactattgt
       61 ggtagaacaa tgtaaaccat aattaatctg cgtgcggata catgcttcat agataggata
      121 ttctggtttg ggttgtgaat gtgttggtca tcgagtggac aataaggtaa gaggagtaac
      181 tcctgcaaaa caaaaaaaga gttcaacaaa atcaatcagt taaaaacacg agagatctgt
      241 ataaaaaagc atcgtaaatt ctgtattttt cctttaaata taatatgtta ttgagtttt
//
LOCUS       DAZFBR010000519          292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFBR010000519 DAZFBR010000000
VERSION     DAZFBR010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.527"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>292
                     /locus_tag="ACHJZ2_005549"
     CDS             <1..>292
                     /locus_tag="ACHJZ2_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HFO4571397.1"
                     /translation="FLSEQCLKCYGLKEDTLSLFVDKTKSNDNNNVFIKKLPDNILQG
                     VVINFAMGAGLREIIKKVYNDTRFTDLTKSQMKILCESKNVNNVPGLLLALQ"
BASE COUNT          111 a           37 c           51 g           93 t
ORIGIN      
        1 attttcttag tgagcaatgt ctgaaatgtt atgggctaaa agaagacaca ctgtcacttt
       61 ttgtggataa aacaaaatcg aatgacaaca ataatgtgtt tataaaaaaa ttacctgata
      121 atattctcca aggtgttgtt attaattttg ctatgggagc aggactaaga gagataataa
      181 aaaaggtata taacgatact cgatttacag atcttacaaa atctcagatg aaaatcttgt
      241 gtgaatcaaa aaatgttaat aatgttcctg ggttgctttt agctctacaa aa
//
LOCUS       DAZFBR010000520          292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFBR010000520 DAZFBR010000000
VERSION     DAZFBR010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.528"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>292
                     /locus_tag="ACHJZ2_005550"
     CDS             <1..>292
                     /locus_tag="ACHJZ2_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571398.1"
                     /translation="VKEFKDDIASMLCEQPPMRTHQISSSGYVSADKLHKLYKTNLED
                     AQRDLKTTPVDIDGVSLKSITREKIAEEIAKKTAEIIAKQNAKSIEEKNIEKN"
BASE COUNT          116 a           54 c           62 g           60 t
ORIGIN      
        1 gtaaaagagt ttaaagacga tatagcctcg atgctttgtg aacagccccc catgcgtaca
       61 caccagattt cttcatccgg atacgtatct gccgataaac tgcacaaact ctataagaca
      121 aatttggaag acgcgcagag agatctgaaa acaacaccgg ttgacatcga tggtgttagt
      181 ctaaaatcaa taactcggga aaaaattgcg gaagaaattg cgaaaaaaac tgcggaaata
      241 attgcgaaac aaaatgcgaa gagcattgag gagaagaata ttgaaaaaaa ca
//
LOCUS       DAZFBR010000521          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFBR010000521 DAZFBR010000000
VERSION     DAZFBR010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.529"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>287
                     /locus_tag="ACHJZ2_005551"
     CDS             <1..>287
                     /locus_tag="ACHJZ2_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO4571399.1"
                     /translation="MKNFKIETSKIYSINQDPQRGDKKVGAYLFMQSFVYASNLQDGV
                     CKEASVIASSLLSCNIAKLNGVGMDFIETSFYGKTRGGFRDSNNFYDCDFR"
BASE COUNT          101 a           32 c           60 g           94 t
ORIGIN      
        1 aaatgaaaaa tttcaagata gagacatcaa aaatatactc tataaatcaa gatccccaaa
       61 gaggggataa gaaagtcggt gcatatctgt ttatgcagtc atttgtatat gcaagtaatc
      121 ttcaagatgg agtgtgtaaa gaagcaagtg ttattgcaag cagtttatta tcttgcaata
      181 tagcaaagct taatggtgtt ggaatggatt tcatagaaac ttcgttttat ggaaaaactc
      241 gcggtggttt tagagatagt aataattttt atgattgtga ttttcgt
//
LOCUS       DAZFBR010000522          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFBR010000522 DAZFBR010000000
VERSION     DAZFBR010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.530"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            24..100
                     /locus_tag="ACHJZ2_005552"
     tRNA            24..100
                     /locus_tag="ACHJZ2_005552"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHJZ2_005553"
     tRNA            143..218
                     /locus_tag="ACHJZ2_005553"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZFBR010000523          272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFBR010000523 DAZFBR010000000
VERSION     DAZFBR010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.531"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>272
                     /locus_tag="ACHJZ2_005554"
     CDS             <1..>272
                     /locus_tag="ACHJZ2_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571400.1"
                     /translation="IQPAIKNGERVPATTNGPFKVNGKWTSGTHYTGGSKRLDAGIID
                     ITFPTNQYGLHPVIEGFDITLNKTKPSAVDIYSDVFGGIDINDFRL"
BASE COUNT           93 a           47 c           54 g           78 t
ORIGIN      
        1 gattcaacca gccataaaaa atggagagcg agttcctgcg acaacgaatg gaccatttaa
       61 agtaaatggt aagtggacat ccggcactca ttatacaggt ggttccaaac gtctagatgc
      121 cggtattatt gatatcacat ttcctacaaa ccaatatgga ttacatccag ttattgaagg
      181 atttgatata acacttaata aaacaaaacc atcagcagtg gatatatatt cagatgtgtt
      241 tggtgggatt gatattaacg actttcggtt at
//
LOCUS       DAZFBR010000524          270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFBR010000524 DAZFBR010000000
VERSION     DAZFBR010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.532"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>270
                     /locus_tag="ACHJZ2_005555"
     CDS             <1..>270
                     /locus_tag="ACHJZ2_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4571401.1"
                     /translation="LSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVD
                     VVTGEITTIQSLRHTSDLGTGEMYVFLCKVEAWAMNIGCHLTIPQ"
BASE COUNT           74 a           67 c           64 g           65 t
ORIGIN      
        1 actcagccac atgtggtaca gcgaaatcag tgaatacctc atcagcaggg ggaaatcgtt
       61 cgctacttca gcatgggtaa aagatgctct caaacacaca tacctcggtt atgaaaccaa
      121 ggacctggtt gatgtcgtaa ccggcgaaat cactactatc cagtcgttac gccatacctc
      181 cgatcttggt accggagaga tgtatgtctt cctgtgtaag gttgaagcct gggcgatgaa
      241 tattggctgc cacctgacta ttccacagag
//
LOCUS       DAZFBR010000525          268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFBR010000525 DAZFBR010000000
VERSION     DAZFBR010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.533"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..>268
                     /gene="nleE"
                     /locus_tag="ACHJZ2_005556"
     CDS             <1..>268
                     /gene="nleE"
                     /locus_tag="ACHJZ2_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO4571402.1"
                     /translation="RGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTP
                     EGSWLTVISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKE"
BASE COUNT           87 a           48 c           59 g           74 t
ORIGIN      
        1 ccagaggagt agctgaacta aaacaggaag aatttgttaa cgagaaagcc agacggtttt
       61 cttatatgaa gactatgtat tctgtatgtc cagaagcgtt tgaacctatt tccagaaatg
      121 aagccagtac accggaagga agctggctaa cagttatatc cggaaaacgc ccaatggggc
      181 agttttctgt agatagttta tacaatcctg atttacatgc attatgtgag cttccggaca
      241 tttgttgtaa gatcttccct aaagaaaa
//
LOCUS       DAZFBR010000526          267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFBR010000526 DAZFBR010000000
VERSION     DAZFBR010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.denovo.534"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            complement(<1..>267)
                     /locus_tag="ACHJZ2_005557"
     CDS             complement(<1..>267)
                     /locus_tag="ACHJZ2_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO4571403.1"
                     /translation="KILTEISKMPEDSIFTKINTRIPRDTMPILRRYHLWPDGWNIRG
                     LNGFMLSHKGSEVIDAVIAGQNQAYRELRRIRDNIHSEIYFKQT"
BASE COUNT           71 a           54 c           45 g           97 t
ORIGIN      
        1 cagtttgttt gaagtatatt tcactatgaa tattatctct tattcttctt agttccctgt
       61 aagcctgatt ctggcctgcg ataacagcat caatcacttc actaccttta tgtgatagca
      121 tgaatccatt gagcccacga atattccatc catcaggcca taggtgataa cgacgaagga
      181 tgggcattgt gtctcgagga atccttgtat tgatcttagt aaaaatacta tcttctggca
      241 ttttagatat ttctgttaat atcttct
//
LOCUS       DAZFBR010000527         4261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.guided.215, whole genome shotgun sequence.
ACCESSION   DAZFBR010000527 DAZFBR010000000
VERSION     DAZFBR010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.guided.215"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..691
                     /gene="frdB"
                     /locus_tag="ACHJZ2_005558"
     CDS             <1..691
                     /gene="frdB"
                     /locus_tag="ACHJZ2_005558"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO4571404.1"
                     /translation="PEVDTAPHSAFYEVPYDATTSLLDALGYIKDNLAPDLSYRWSCR
                     MAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERDLVVDMTHFIESLEAI
                     KPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAIT
                     LAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESS
                     KDFLIATLKPR"
     gene            702..1097
                     /gene="frdC"
                     /locus_tag="ACHJZ2_005559"
     CDS             702..1097
                     /gene="frdC"
                     /locus_tag="ACHJZ2_005559"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO4571405.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1108..1467
                     /gene="frdD"
                     /locus_tag="ACHJZ2_005560"
     CDS             1108..1467
                     /gene="frdD"
                     /locus_tag="ACHJZ2_005560"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO4571406.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1530..2663
                     /locus_tag="ACHJZ2_005561"
     CDS             1530..2663
                     /locus_tag="ACHJZ2_005561"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4571407.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            2751..3284
                     /gene="blc"
                     /locus_tag="ACHJZ2_005562"
     CDS             2751..3284
                     /gene="blc"
                     /locus_tag="ACHJZ2_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO4571408.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3281..3598)
                     /gene="sugE"
                     /locus_tag="ACHJZ2_005563"
     CDS             complement(3281..3598)
                     /gene="sugE"
                     /locus_tag="ACHJZ2_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO4571409.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3774..3920)
                     /gene="ecnB"
                     /locus_tag="ACHJZ2_005564"
     CDS             complement(3774..3920)
                     /gene="ecnB"
                     /locus_tag="ACHJZ2_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO4571410.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(4031..4156)
                     /gene="ecnA"
                     /locus_tag="ACHJZ2_005565"
     CDS             complement(4031..4156)
                     /gene="ecnA"
                     /locus_tag="ACHJZ2_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO4571411.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
BASE COUNT         1061 a         1099 c         1095 g         1006 t
ORIGIN      
        1 cccggaagtc gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac
       61 ctcattactg gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagctaccg
      121 ctggtcctgc cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa
      181 actggcatgt aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc
      241 taacttcccg attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga
      301 agcgatcaaa ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca
      361 gaccccggcg cagatggcga agtatcacca gttctccggt tgcatcaact gtggtctgtg
      421 ctacgccgcg tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac
      481 gctggcgcat cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca
      541 gttgaacagc cagaacggcg tatggagctg tactttcgtg ggctactgct ccgaagtctg
      601 cccgaaacac gtcgatccgg ctgcggccat tcaacagggc aaagtagaaa gttcgaaaga
      661 ctttcttatc gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac
      721 cgtatgtacg gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca
      781 tgctgcgcga aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc
      841 tgtttgccct gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc
      901 cggttattgt gatcattaac ctgatcactc tggcggcagc tctgctgcac accaaaacct
      961 ggtttgaact ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag
     1021 agccaattat caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg
     1081 ttgccctgta ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa
     1141 ccggtattct ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg
     1201 atgatcctgc tggtgggtat tctgctgccg ctggggctgt ttccgggtga tgcactgagc
     1261 tacgagcgcg ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg
     1321 atcgttctgc cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa
     1381 atccacgtac ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc
     1441 acgctgattg gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg
     1501 cgggccgttt tgtatggaaa ccagacccta tgttcaaaac gacgctctgc gccttattaa
     1561 ttaccgcctc ttgctccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca
     1621 caattacccc gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc
     1681 agggtaaacc ttattacttt acctgggggt atgcggacat cgccaaaaag cagcccgtca
     1741 cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg
     1801 gcgacgctat tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg
     1861 aacttaccgc taaacagtgg aatgggatca cactattaca tctcgcaacc tacactgctg
     1921 gcggcctgcc attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct
     1981 atcaaaactg gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta
     2041 tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc
     2101 aaactcgtgt cttccagcca ctcaaactca accatacgtg gattaatgta ccgcccgcag
     2161 aagaaaagaa ttacgcctgg ggatatcgcg aaggcaaggc agttcatgtt tcgccagggg
     2221 cgttagatgc tgaaacttat ggtgtgaagt cgaccattga agatatggcc tgctgggtac
     2281 gaagcaatat gaatccccgt gatatcaacg acaaaacact tcagcaaggg atacaactgg
     2341 cacaatctcg ctactggcaa accggcgata tgtatcaggg tctgggctgg gaaatgctgg
     2401 actggccggt aaatcctgac atcatcgtta acggcagtga caataaaatt gcactggcag
     2461 cacgccccgt aaaagcgatt acgcctccaa ctcctgcagt acgagcatca tgggtgcata
     2521 aaacgggggc gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg
     2581 gtatcgtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctggc
     2641 agattctcaa cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg
     2701 cttttccttg ctgtcatcta cacttagaaa aaaccagtaa ggaaacattt atgcgcctgc
     2761 tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc
     2821 cgccgcgtgg cgtgaccgta gtgaataatt tcgacgctaa acgctatctc ggtacctggt
     2881 atgagattgc ccgttttgat caccgctttg aacgtggact ggaaaaagtc accgcaacat
     2941 acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag
     3001 gaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc
     3061 tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg
     3121 aataccgcca tgcgctggtt tgcggaccgg accgcgacta cctgtggata ctctcccgca
     3181 cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt
     3241 ttgatgtcag taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca
     3301 gaccaataat ccccaatacg attaacgcca gactcgccag acgcatcggg ttagcggact
     3361 caccgagcag cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg
     3421 cataagccgt ccctactggt aacgatttca tcgcccaggc aagtagcgcc atactgacaa
     3481 tcatcgccgt cacggtaata acactcggcg tcaaacgact aaagccgtgg gtatatttca
     3541 ggcctacggc ccataccact tccagcagac cagcaataac taagataatc caggacatat
     3601 caggctccgg agaattgggg ccgtccccgg tgaaaagacg cgtttgcagg tcgtcctgca
     3661 aagctgatgg ttgaacggat attttgccag gtggcggggg aatttcaact attacctttg
     3721 tgactatcgc agaaaaaaca aacggcacga cacagctgtc gtaccgttat tgcttattgc
     3781 tgcgctttcg ttgctgcacc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg
     3841 ccacgcgtgg tgttgcaggc agttaatact gttgaaagca ccagaacaga aaagatcgct
     3901 gcaattgtct tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat
     3961 agcaaattaa taatagacaa catcgccaaa aatgatggaa atctgatgat ttttcggaag
     4021 agaagaaaaa ttagctggca gcgcgggaga tggagttgcc gagatgtttg atgtcttcgc
     4081 cgaaaccgcg agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga
     4141 taaggcgttt catcatctct gacctgaata agtgatggtg cagcctgcag gccgcaccac
     4201 aaccgcatta cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt
     4261 c
//
LOCUS       DAZFBR010000528         4261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.guided.216, whole genome shotgun sequence.
ACCESSION   DAZFBR010000528 DAZFBR010000000
VERSION     DAZFBR010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.guided.216"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..691
                     /gene="frdB"
                     /locus_tag="ACHJZ2_005566"
     CDS             <1..691
                     /gene="frdB"
                     /locus_tag="ACHJZ2_005566"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO4571412.1"
                     /translation="PEVDTAPHSAFYEVPYDATTSLLDALGYIKDNLAPDLSYRWSCR
                     MAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERDLVVDMTHFIESLEAI
                     KPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAIT
                     LAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESS
                     KDFLIATLKPR"
     gene            702..1097
                     /gene="frdC"
                     /locus_tag="ACHJZ2_005567"
     CDS             702..1097
                     /gene="frdC"
                     /locus_tag="ACHJZ2_005567"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO4571413.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1108..1467
                     /gene="frdD"
                     /locus_tag="ACHJZ2_005568"
     CDS             1108..1467
                     /gene="frdD"
                     /locus_tag="ACHJZ2_005568"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO4571414.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1530..2663
                     /locus_tag="ACHJZ2_005569"
     CDS             1530..2663
                     /locus_tag="ACHJZ2_005569"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4571415.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREVKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            2751..3284
                     /gene="blc"
                     /locus_tag="ACHJZ2_005570"
     CDS             2751..3284
                     /gene="blc"
                     /locus_tag="ACHJZ2_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO4571416.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3281..3598)
                     /gene="sugE"
                     /locus_tag="ACHJZ2_005571"
     CDS             complement(3281..3598)
                     /gene="sugE"
                     /locus_tag="ACHJZ2_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO4571417.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3774..3920)
                     /gene="ecnB"
                     /locus_tag="ACHJZ2_005572"
     CDS             complement(3774..3920)
                     /gene="ecnB"
                     /locus_tag="ACHJZ2_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO4571418.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(4031..4156)
                     /gene="ecnA"
                     /locus_tag="ACHJZ2_005573"
     CDS             complement(4031..4156)
                     /gene="ecnA"
                     /locus_tag="ACHJZ2_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO4571419.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
BASE COUNT         1061 a         1099 c         1094 g         1007 t
ORIGIN      
        1 cccggaagtc gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac
       61 ctcattactg gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagctaccg
      121 ctggtcctgc cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa
      181 actggcatgt aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc
      241 taacttcccg attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga
      301 agcgatcaaa ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca
      361 gaccccggcg cagatggcga agtatcacca gttctccggt tgcatcaact gtggtctgtg
      421 ctacgccgcg tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac
      481 gctggcgcat cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca
      541 gttgaacagc cagaacggcg tatggagctg tactttcgtg ggctactgct ccgaagtctg
      601 cccgaaacac gtcgatccgg ctgcggccat tcaacagggc aaagtagaaa gttcgaaaga
      661 ctttcttatc gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac
      721 cgtatgtacg gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca
      781 tgctgcgcga aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc
      841 tgtttgccct gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc
      901 cggttattgt gatcattaac ctgatcactc tggcggcagc tctgctgcac accaaaacct
      961 ggtttgaact ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag
     1021 agccaattat caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg
     1081 ttgccctgta ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa
     1141 ccggtattct ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg
     1201 atgatcctgc tggtgggtat tctgctgccg ctggggctgt ttccgggtga tgcactgagc
     1261 tacgagcgcg ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg
     1321 atcgttctgc cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa
     1381 atccacgtac ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc
     1441 acgctgattg gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg
     1501 cgggccgttt tgtatggaaa ccagacccta tgttcaaaac gacgctctgc gccttattaa
     1561 ttaccgcctc ttgctccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca
     1621 caattacccc gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc
     1681 agggtaaacc ttattacttt acctgggggt atgcggacat cgccaaaaag cagcccgtca
     1741 cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg
     1801 gcgacgctat tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg
     1861 aacttaccgc taaacagtgg aatgggatca cactattaca tctcgcaacc tacactgctg
     1921 gcggcctgcc attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct
     1981 atcaaaactg gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta
     2041 tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc
     2101 aaactcgtgt cttccagcca ctcaaactca accatacgtg gattaatgta ccgcccgcag
     2161 aagaaaagaa ttacgcctgg ggatatcgcg aagtcaaggc agttcatgtt tcgccagggg
     2221 cgttagatgc tgaaacttat ggtgtgaagt cgaccattga agatatggcc tgctgggtac
     2281 gaagcaatat gaatccccgt gatatcaacg acaaaacact tcagcaaggg atacaactgg
     2341 cacaatctcg ctactggcaa accggcgata tgtatcaggg tctgggctgg gaaatgctgg
     2401 actggccggt aaatcctgac atcatcgtta acggcagtga caataaaatt gcactggcag
     2461 cacgccccgt aaaagcgatt acgcctccaa ctcctgcagt acgagcatca tgggtgcata
     2521 aaacgggggc gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg
     2581 gtatcgtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctggc
     2641 agattctcaa cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg
     2701 cttttccttg ctgtcatcta cacttagaaa aaaccagtaa ggaaacattt atgcgcctgc
     2761 tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc
     2821 cgccgcgtgg cgtgaccgta gtgaataatt tcgacgctaa acgctatctc ggtacctggt
     2881 atgagattgc ccgttttgat caccgctttg aacgtggact ggaaaaagtc accgcaacat
     2941 acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag
     3001 gaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc
     3061 tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg
     3121 aataccgcca tgcgctggtt tgcggaccgg accgcgacta cctgtggata ctctcccgca
     3181 cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt
     3241 ttgatgtcag taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca
     3301 gaccaataat ccccaatacg attaacgcca gactcgccag acgcatcggg ttagcggact
     3361 caccgagcag cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg
     3421 cataagccgt ccctactggt aacgatttca tcgcccaggc aagtagcgcc atactgacaa
     3481 tcatcgccgt cacggtaata acactcggcg tcaaacgact aaagccgtgg gtatatttca
     3541 ggcctacggc ccataccact tccagcagac cagcaataac taagataatc caggacatat
     3601 caggctccgg agaattgggg ccgtccccgg tgaaaagacg cgtttgcagg tcgtcctgca
     3661 aagctgatgg ttgaacggat attttgccag gtggcggggg aatttcaact attacctttg
     3721 tgactatcgc agaaaaaaca aacggcacga cacagctgtc gtaccgttat tgcttattgc
     3781 tgcgctttcg ttgctgcacc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg
     3841 ccacgcgtgg tgttgcaggc agttaatact gttgaaagca ccagaacaga aaagatcgct
     3901 gcaattgtct tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat
     3961 agcaaattaa taatagacaa catcgccaaa aatgatggaa atctgatgat ttttcggaag
     4021 agaagaaaaa ttagctggca gcgcgggaga tggagttgcc gagatgtttg atgtcttcgc
     4081 cgaaaccgcg agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga
     4141 taaggcgttt catcatctct gacctgaata agtgatggtg cagcctgcag gccgcaccac
     4201 aaccgcatta cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt
     4261 c
//
LOCUS       DAZFBR010000529         3554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.guided.236, whole genome shotgun sequence.
ACCESSION   DAZFBR010000529 DAZFBR010000000
VERSION     DAZFBR010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.guided.236"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            <1..364
                     /gene="prfH"
                     /locus_tag="ACHJZ2_005574"
     CDS             <1..364
                     /gene="prfH"
                     /locus_tag="ACHJZ2_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO4571420.1"
                     /translation="HHGRKNWFLGIGRFTADEQEQSDAIRYETLRPSGPGGQHVNKTD
                     SAVRATHLATGISVKVQSERSQHANKRLARLLIAWKLEQQQQENSAVLKSQRRMFHHQ
                     IERGNPRRTFTGMAFIEG"
     gene            complement(421..1878)
                     /gene="pepD"
                     /locus_tag="ACHJZ2_005575"
     CDS             complement(421..1878)
                     /gene="pepD"
                     /locus_tag="ACHJZ2_005575"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO4571421.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2139..2597
                     /gene="gpt"
                     /locus_tag="ACHJZ2_005576"
     CDS             2139..2597
                     /gene="gpt"
                     /locus_tag="ACHJZ2_005576"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO4571422.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2689..>3554
                     /gene="frsA"
                     /locus_tag="ACHJZ2_005577"
     CDS             2689..>3554
                     /gene="frsA"
                     /locus_tag="ACHJZ2_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO4571423.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPR"
BASE COUNT          832 a          925 c          935 g          862 t
ORIGIN      
        1 tcatcatggg cgcaaaaact ggtttctggg cattgggcgt tttaccgctg atgagcagga
       61 acaatcggat gcaatccgtt atgagacgct gcgtccgtcg gggccgggcg gtcaacatgt
      121 caataaaacc gactcggcgg tacgcgccac gcatctggca accgggatta gcgtgaaggt
      181 tcagtcagaa cgcagccagc atgctaacaa acggctggcg cgattgctga ttgcctggaa
      241 gctggagcag cagcaacagg aaaatagcgc agtgctgaaa tcgcagcggc gaatgttcca
      301 tcaccagatt gaacgtggca acccgcgacg aacgttcaca gggatggctt ttatcgaagg
      361 ataatgaagg atgaaactgc cggaaggcga ttaaacgcca tccggcagca aatcaaataa
      421 ttacttcgcc ggaatttctt tcagcagttc agtcagcagt gtccagtaat gacctacgct
      481 ttcgatgtga acttgctcat ccggagagtg tggaccggtg attgttggcc cgatagaaac
      541 catgtccatt tccggatacg gttttttgaa cagaccacat tccaggcccg cgtggataat
      601 ctggatgttc ggcgtcttgt tgaacaggcg ctgataggtt tcacgtacca gatgcatcac
      661 cggagaatta gcgtccggct gccagccagg atatgcgcct ttcgcttcgg ttttcgcgcc
      721 agccagttta cccagcgaat ccagcatgct caccacgtag tctttaccgc tgtcgatcag
      781 agaacggatc agacagtgaa tttctacgtt attgtcagtc atggtcacca caccgacgtt
      841 cagggaggtt tcgaccacac cttttgccac gtcggagttg cggatcacac cgttcggggt
      901 ggcgttcagc agacgaataa aggtatcgcg agatttcgca atcagggcag ctttatcgtt
      961 cgctacagag tccagcaaca aggccagatt tttctctttc tctgccagct cgtttttcag
     1021 gatctcctga taggtattca ccagagattt cagggcgtcg actttatcag ctgcgacagc
     1081 aatggtcgca aaggcttcac gcgggatggc gttacgcagt gtgccgccgt tgaaatcaat
     1141 aagacgcagg tccagttctt ctgcatgacc cgccaggaag cgcaccagca gtttgttggc
     1201 attacccagg ccaacgtgga tttccccgcc ggagtgaccg cctttcagac cttttaaggt
     1261 taacttgaag gtttcaaaac cagctggaac cgcttcacga tctaaatgca ggttggaggt
     1321 gaagtcgata ccccccgcgc aacccatgta gatttcacct tcttcttcgg agtcggtgtt
     1381 aatcagaata tcagcctgca accagttgct ctgtaagccg aacgcaccgt ccataccggc
     1441 ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt cgtcagccag
     1501 aaccgccagt gcagaggcca taccaatgcc gttatccgca cccagcgtgg tgccgcgcgc
     1561 tttaacccat tcgccatcaa tatatggctg gataggatct ttcgtgaagt catgcacggt
     1621 gtcgttattt ttctgcggca ccatatcgag gtgggcctgc aagacgaccg gtttacgatt
     1681 ttccatacct gcggtagcag gtttacgaat caggatatta cctacctgat cgcgttcgac
     1741 atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct cttcatgata
     1801 ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct gtggagataa
     1861 ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg
     1921 tcacagggtt agcagaaaat gttgtcaaca caagacaggc ttgcgagata tgtttgagaa
     1981 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
     2041 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
     2101 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
     2161 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
     2221 aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggtgcg ttactggcgc
     2281 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
     2341 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
     2401 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
     2461 actttgtcac catctttgca aaaccggctg gtcgtccgct ggttgatgat tatgttgttg
     2521 atatcccgca agatacctgg atcgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
     2581 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
     2641 tcaggcgagt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
     2701 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
     2761 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
     2821 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc
     2881 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat
     2941 ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccacccag
     3001 gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc cgcaactcag tggtcgtcac
     3061 tggctgcatg cggctacgtt gtacaacatt gccgcctatc ctcatctgaa aggagatgac
     3121 ctggccgagc aagcgcaggc tttgtcaaac cgcgcctatg aagaggccgc tcagcgtcta
     3181 ccgggcacga tgcgacagat ggagtttacc gtacccggcg gtgcgcccat caccggcttt
     3241 ttgcatatgc cgaaaggcga tggcccgttc ccgacggtat taatgtgtgg tggtctggat
     3301 gcgatgcaga cggactatta cagcctgtat gaacgttatt ttgcgccgcg cggcattgcg
     3361 atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct cacccaggac
     3421 tcctgcctgt tgcatcagcg cgtcttaaag gctctgccta acgtaccgtg ggtggatcac
     3481 actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg tctggcatat
     3541 cttgaatcgc cgcg
//
LOCUS       DAZFBR010000530         1041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.guided.372, whole genome shotgun sequence.
ACCESSION   DAZFBR010000530 DAZFBR010000000
VERSION     DAZFBR010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.guided.372"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            6..965
                     /locus_tag="ACHJZ2_005578"
     CDS             6..965
                     /locus_tag="ACHJZ2_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO4571424.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..>1041
                     /locus_tag="ACHJZ2_005579"
     CDS             977..>1041
                     /locus_tag="ACHJZ2_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin subunit B"
                     /protein_id="HFO4571425.1"
                     /translation="MKKMFMAVLFALVSVNAMAAD"
BASE COUNT          279 a          206 c          255 g          301 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggt ttttcttcgg
       61 tatcctattc ccgggaattt acgatagact tttcgactca acaaagttat gtctcttcgt
      121 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccgccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccgatgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacggcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcagc gatactgggt actgtggccg
      781 ttatactgaa ttgccatcat cagggggcgc gttctgttcg cgccgtgaat gaagagagtc
      841 aaccagaatg tcagataact ggcgacaggc ccgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagtatga agaagatgtt tatggcggtt ttatttgcat tagtttctgt
     1021 taatgcaatg gcggcggatt g
//
LOCUS       DAZFBR010000531          876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.guided.399, whole genome shotgun sequence.
ACCESSION   DAZFBR010000531 DAZFBR010000000
VERSION     DAZFBR010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.guided.399"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            6..>876
                     /gene="stxA2"
                     /locus_tag="ACHJZ2_005580"
     CDS             6..>876
                     /gene="stxA2"
                     /locus_tag="ACHJZ2_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO4571426.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPV"
BASE COUNT          222 a          188 c          216 g          250 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggc ttttcttcgg
       61 tatcctattc ccgggaattt acgatagact tttcgactca acaaagttat gtatcttcgt
      121 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcggc gatactgggc actgtggccg
      781 ttatactgaa ttgtcatcat cagggggcgc gttctgttcg cgccgtgaat gaagatagtc
      841 aaccagaatg tcagataact ggcgacaggc ccgtta
//
LOCUS       DAZFBR010000532          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.guided.496, whole genome shotgun sequence.
ACCESSION   DAZFBR010000532 DAZFBR010000000
VERSION     DAZFBR010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.guided.496"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            71..340
                     /locus_tag="ACHJZ2_005581"
     CDS             71..340
                     /locus_tag="ACHJZ2_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HFO4571427.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          131 a           70 c          105 g          122 t
ORIGIN      
        1 atacagctgc agcgtttctg aacagaaagt cacagttttt atatacaacg ggtaaataaa
       61 ggagttaagt atgaagaaga tgtttatggc ggttttattt gcattagttt ctgttaatgc
      121 aatggcggcg gattgcgcta aaggtaaaat tgagttttcc aagtataatg agaatgatac
      181 attcacagta aaagtggccg gaaaagagta ctggaccagt cgctggaatc tgcaaccgtt
      241 actgcaaagt gctcagttga caggaatgac tgtcacaatc aaatccagta cctgtgaatc
      301 aggctccgga tttgctgaag tgcagtttaa taatgactga ggcataacct gattcgtggt
      361 atgtgggtaa caagtgtaat ctgtgtcaca attcagtcag ttgacagttg cctgtcagac
      421 tgagcatt
//
LOCUS       DAZFBR010000533          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2212W9809
            SAMN43283607-rid23064843.guided.497, whole genome shotgun sequence.
ACCESSION   DAZFBR010000533 DAZFBR010000000
VERSION     DAZFBR010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283607
            Sequence Read Archive: SRR30325141
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351403.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:27:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 110 of 266
            Pseudo Genes (incomplete)         :: 134 of 266
            Pseudo Genes (internal stop)      :: 67 of 266
            Pseudo Genes (multiple problems)  :: 37 of 266
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283607-rid23064843.guided.497"
                     /serotype="O157:H 7"
                     /isolate="2212W9809"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx2a + stx2c"
     gene            71..340
                     /locus_tag="ACHJZ2_005582"
     CDS             71..340
                     /locus_tag="ACHJZ2_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO4571428.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          129 a           68 c          107 g          124 t
ORIGIN      
        1 atacagctgc agcgtttctg aacagaaagt cacagttttt atatacaacg ggtaaataaa
       61 ggagttaagt atgaagaaga tgtttatggc ggttttattt gcattagttt ctgttaatgc
      121 aatggcggcg gattgtgcta aaggtaaaat tgagttttcc aagtataatg aggatgacac
      181 atttacagtg aaggttgacg ggaaagaata ctggaccagt cgctggaatc tgcaaccgtt
      241 actgcaaagt gctcagttga caggaatgac tgtcacaatc aaatccagta cctgtgaatc
      301 aggctccgga tttgctgaag tgcagtttaa taatgactga ggcataacct gattcgtggt
      361 atgtgggtaa caagtgtaat ctgtgtcaca attcagtcag ttgacagttg cctgtcagac
      421 tgagcatt
//
